LOCUS       DASWGM010000001         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2633535,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000001 DASWGM010000000
VERSION     DASWGM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633535"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /gene="gatB"
                     /locus_tag="VGA15_00005"
     CDS             complement(<1..887)
                     /gene="gatB"
                     /locus_tag="VGA15_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX9586114.1"
                     /translation="MSATSSKLIKGATGDWEMVIGLEVHAQVASNSKLFSGASTEFGG
                     EPNSHVSLVDAAMPGMLPVINEFCVKQAVRTGLGLNAQINRRSVFDRKNYFYPDLPQG
                     YQISQYKSPIVGEGEVVVELAGGESVAVGIERLHLEQDAGKSLHDQSPTMSFVDLNRS
                     GVALMEIVSKPDIRSSEQAKAYVAKLRSILRYLGTCDGDMEKGSLRADVNVSVRKPGG
                     PLGTRCEIKNMNSISFIGDAIEYEARRQIEILEDGGAIDQETRLFDPNKGETRSMRSK
                     EEAHDYRYFPDPDLLPLEF"
     gene            complement(884..1177)
                     /locus_tag="VGA15_00010"
     CDS             complement(884..1177)
                     /locus_tag="VGA15_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586115.1"
                     /translation="MADFKASLSGAAPAPGLDAPLAALWWAAKGNWNEAHKIAQDHQT
                     PDAAWVHAYLHRAEGDLGNAGYWYRQAGKPVAAGSLEIEWEQMVSALLGSGRA"
     gene            complement(1180..2652)
                     /gene="gatA"
                     /locus_tag="VGA15_00015"
                     /pseudo
     CDS             complement(1180..2652)
                     /gene="gatA"
                     /locus_tag="VGA15_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562950.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
     gene            complement(2649..2861)
                     /locus_tag="VGA15_00020"
     CDS             complement(2649..2861)
                     /locus_tag="VGA15_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586116.1"
                     /translation="MCLLCDDEKAYAAYMNYLDAMARQGKVADPDKAIDAVLDQLEAA
                     DKANRKSDDPANDKTLSPFFCSPVNE"
     gene            complement(3004..3291)
                     /gene="gatC"
                     /locus_tag="VGA15_00025"
     CDS             complement(3004..3291)
                     /gene="gatC"
                     /locus_tag="VGA15_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX9586117.1"
                     /translation="MSVDAATVRRIAHLARIAVTDAEVPHLQGELNAMLAFVEQLSEV
                     NVEGVEPMTSVMPMEMKKRPDVVNDGEIADDIVRNAPATEAHFFLVPKVVE"
     gene            complement(3596..>4569)
                     /locus_tag="VGA15_00030"
     CDS             complement(3596..>4569)
                     /locus_tag="VGA15_00030"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9586118.1"
                     /translation="QGGALGSLPCAMMSAEKAREQINIIRQRVSAPVNMNFFCHKAVD
                     ADSQREAGWKKRLGPYYEELGLDPAAPVNAANRAPFDAAMCELVEELKPEVVSFHFGL
                     PDQALLRRVKAAGCIVISSATIVREAIWLEENGADAIIAQGADAGGHRGMFLTENIAE
                     QPGTFALVPQVVDAVKVPVIAAGGIADGRGIAAAFALGAAGVQIGSAYLRCPESKVIA
                     PARVALAQARDDSTVITNVMTGRPARGVANRVMREVGPISPDAPAFPHAATGLAPLKT
                     AAEKLGKVDFTNLWAGQAVRMGREMPAAELTRALAGAALARLSRMAG"
BASE COUNT          800 a         1511 c         1377 g          881 t
ORIGIN      
        1 ctgaactcca gcggcaacag atcggggtcc gggaaatagc gatagtcgtg cgcctcttcc
       61 ttggagcgca tcgaacgggt ctcgcccttg ttgggatcga acagccgggt ttcctgatcg
      121 atcgcgccgc cgtcttcgag gatttcgatc tggcgccgcg cctcatattc gatggcgtcg
      181 ccgatgaagc tgatcgagtt catgttcttg atctcgcagc gggtgccgag cgggccgccc
      241 ggcttgcgca cggagacgtt gacgtcggcg cgcaaggaac ccttctccat gtcgccgtcg
      301 caggtgccga ggtagcgcag gatcgaacgc aattttgcca cataggcttt ggcctgctcc
      361 gacgagcgga tatcgggctt ggagacgatc tccatcagcg cgacaccgga tcggttgagg
      421 tcgacgaacg acatcgtcgg gctctggtcg tgcagcgact tgccggcatc ctgctccaga
      481 tgcagccgct cgatgcccac agcgacactc tcgccgcccg ccagctcgac aaccacctcg
      541 ccctcgccca cgatcggcga cttgtactgg ctgatctgat agccttgcgg caaatccgga
      601 tagaaatagt tcttgcggtc gaataccgaa cgccgattga tctgcgcgtt caggccaagg
      661 ccggtgcgca ccgcctgctt gacgcaaaac tcgttgataa ccggcagcat gcccggcatc
      721 gccgcgtcga ccagcgagac gtggctgttc ggctcgccgc cgaattccgt cgaggcgccg
      781 gaaaacagtt ttgagttcga ggcgacctgg gcgtggactt ccagcccgat caccatttcc
      841 caatcgccgg tggcgccttt tatcagtttt gagcttgtcg cactcatgct cttccactcc
      901 cgagcagtgc ggaaaccatc tgctcccatt caatttcgag cgaaccggcc gcgaccggct
      961 tgcccgcctg ccggtaccaa tagccagcat tgcccagatc gccctcggca cgatgcagat
     1021 aagcatggac ccaggcagcg tcaggggtct ggtgatcctg cgcgatcttg tgcgcctcgt
     1081 tccagttgcc tttggcggcc caccacagcg cggccagcgg cgcatcgaga cccggcgcag
     1141 gtgccgcgcc cgacaaactc gctttgaaat ctgccattct caccaccacc tcgctggcgt
     1201 gaaccgcccc gccgcctgtt cgatcacctc accgagcgag aacagcgtct cttcgtcgaa
     1261 cggacggccg atcagttgca ggccgagcgg caacgcttgc gcatccctgc cggcgggaac
     1321 cgcgattccc ggcagccccg ccatgttcac ggtcaccgtg aagacgtcgt tgagatacat
     1381 ctccacgggg tcggcgccct tttagccgat gccgaacgct gccgaaggcg tcgccggcgt
     1441 caggatcgcg ttcacgcctt tggcgaaaca atcctcaaaa tccttcttga tcagggtgcg
     1501 gactttttgc gcgcgcagat aataggcgtc gtagtaaccg gcggagagca cgtaggtgcc
     1561 gatcatgacg cggcggcgga cttcggcgcc aaaaccttcg gcgcgggtat tttcgtacat
     1621 gtcgccgatg ctgcggccgg gcacccgcag gccgtagcgc acaccgtcat agcgcgccag
     1681 attcgacgac gcctcggcgg gcgcgacgat gtaataggcc ggcagcgcgt atttggtgtg
     1741 cggcagcgac acatcgacca gctcggcgcc cgcggccttc agccacgccg cgccctcggc
     1801 ccagagcttt tcgatttccg cgggcatgcc gtcgaggcga tactccttgg ggatgccgat
     1861 cttcatgccc ttgaccgatt tcccgatcgc ggcctcataa tccggcaccg cgcgatcgac
     1921 tgacgttgtg tccttggggt cgtgaccggc catcgagcgc agcaggatcg ccgcatcccg
     1981 caccgtccgc gcgatcggcc cggcctgatc gagcgaggat gcgaacgcga cgatgcccca
     2041 gcgcgaacag cggccatagg tcggcttgat tccgacagtc gcggtgaacg ccgccggctg
     2101 gcggatcgag ccgccggtgt cggtcgcggt cgcgcccatg cacaacagcg ccgccaccgc
     2161 cgacgccgag ccgccggacg agccgccggg caccagggtc gtattggagc cttcgcgccg
     2221 ccacggattg acgacggggc cgaagcacga ggtttcgttc gacgagccca tggcgaactc
     2281 gtcattgttg agcttgccga gcatgacggc gccatcgcgc cagagctgcg aggtcacggt
     2341 tgattcatag gtcggcacga aattgccgag aattttcgag cacgcggtgg tccgcacgtc
     2401 cttggtcgcg aacaggtcct tgatgccgag cggaatgccg gcgagcggcc cgccctcacc
     2461 gctggcgatc ttcgcatcgg cttggtgcgc catgccccgc gcctgatccg gcgtttccat
     2521 cacaaacgca ttgagcgagc gcgccgcctc gatcgcggca aggtgcgcgt cggtcagctc
     2581 aagtgacgtg aaggatttgc ccgcaaggcc acttctggcc tcggcgagcg tcagcgatgt
     2641 caaatcggtc attcattaac cgggctgcag aagaagggcg atagcgtctt gtcgttggcc
     2701 ggatcgtcgc ttttacggtt ggccttgtcg gcggcttcga gctggtcgag cacggcgtcg
     2761 atagccttgt cgggatcggc gaccttgccc tgacgcgcca tcgcgtcgag atagttcatg
     2821 taggccgcgt aggccttttc atcgtcgcag agcagacaca tcggatttcc cgcctcgaag
     2881 actcttttgc ttaacctatt ttcttcacgc gaacagctat ccacttcgcc cgaaacgttt
     2941 tagttggttt gacgcgtttt cttcacgcga accggtacct gcttctctcg caaacgcgct
     3001 gatctattcg acgactttcg gcaccaggaa gaaatgagct tccgtggccg gcgcgttcct
     3061 gacgatgtcg tcggcgattt cgccgtcatt gaccacgtcg ggacgcttct tcatttccat
     3121 cggcatgacc gaggtcatcg gttcgacccc ctcgacattc acttcagaca gttgctcgac
     3181 aaaggccagc atcgcattca attcgccctg cagatgtgga acttctgcgt cggtgaccgc
     3241 gatccgcgcg agatgcgcga tacggcgaac ggtggcggca tcgaccgaca tgatatatgg
     3301 cccctgaacg gcgaattcag cccgccgtat agcagagggt gcttttgcac cgcaaccgca
     3361 gccgtcgccc tatgtatcag caacggatga gatcattttc ggtatctaga catcgggagc
     3421 aagttccctc tcccccaaac ttaggtttac ccgagtttgg ctctcaaatt gtcgaagtcg
     3481 gatatatccg acttcgattg ggagagggat gtgggttgcg ctctatcgtt aggccgccgc
     3541 ccctcacccg gattgctctg caatccgacc tctcccgatg ggagaggtga agaaatcagc
     3601 cggccatccg gctcagccgc gccagcgcag cacctgccag cgcccgggtc agctcggcgg
     3661 cgggcatttc gcggcccatc cgcaccgcct gcccggccca gagattggtg aaatcgactt
     3721 ttcccagttt ctccgccgcg gttttcaggg gcgccaagcc ggtggcggcg tggggaaacg
     3781 ccggcgcatc cggcgagatc gggccgacct cgcgcatcac acggttggca accccgcgcg
     3841 ccggacgtcc ggtcatcacg ttggtgataa cggtggaatc atcccgcgcc tgggcgagtg
     3901 ccacccgtgc cggcgcaatc accttggatt ccggacagcg cagataggcg ctgccgattt
     3961 gcacgccggc cgcacccagc gcgaacgccg ccgcaatgcc ccggccgtcg gcgatgccgc
     4021 ccgccgcgat caccggcacc ttcaccgcgt cgaccacctg cggcaccagc gcgaaggtgc
     4081 cgggctgctc ggcgatattc tcggtcagga acatgccgcg atggcctccg gcatccgcgc
     4141 cctgcgcgat aatggcgtcg gcgccgtttt cctccagcca gatcgcctcg cgcacgatgg
     4201 tcgcagacga aatcacgata cagccggcgg ccttgacgcg cctcagcaat gcctgatccg
     4261 gcaggccgaa atggaagctg accacttcgg gcttgagctc ctcgacgagt tcacacatcg
     4321 cggcatcgaa cggcgcgcgg ttggcggcgt tgaccggcgc cgcggggtcg agacccaact
     4381 cttcatagta gggcccaagc cgctttttcc atccggcctc gcgctgagaa tcggcatcga
     4441 ccgccttgtg gcaaaaaaag ttcatgttga ccggcgccga cacgcgctgc ctgatgatgt
     4501 tgatttgctc ccgtgccttt tccgccgaca tcatggcaca gggcagcgat cccagcgctc
     4561 cgccttggg
//
LOCUS       DASWGM010000002         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1109283,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000002 DASWGM010000000
VERSION     DASWGM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109283"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            629..1837
                     /locus_tag="VGA15_00035"
     CDS             629..1837
                     /locus_tag="VGA15_00035"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9586119.1"
                     /translation="MGPLQGIKVVDMTTVLKGPYATQMLGDYGADVIKIETTDGDVTR
                     QIGPTRHPGMGPVFLNTNRSKRSVCLDLKKAAGRDAVLRLIASADVLVYNVRPQAMVR
                     LQLGYDVVSKINPRLIYAGVFGFGQDGPYAAKPAYDDLIQGATALPALMAQTNDGVPR
                     YVPNALVDRIVGLTAVGAICASLVHRERSGRGQRVDIPMFETMAGFVMGDHMGGLTYE
                     PPLDRGGYARHLARDRRPYKTLDGYICVIVYNDKQWENFFKATGRDDLRSDPKFATFA
                     GRAVNIDTVYGELARIFETRTTAEWTSLLENADVPVMPMHDLESILQDPHLVATDFFS
                     VVVHPTEGPIRNMKVAATWSETFADPTRLAPRLNQHGAEILREAGFSTDEIATLERDG
                     VTRAPHSPQD"
     gene            1842..>2752
                     /locus_tag="VGA15_00040"
     CDS             1842..>2752
                     /locus_tag="VGA15_00040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9586120.1"
                     /translation="MDFALTANQESIRDAVARICARFDDAYWLKKDKEGGFPADFHKA
                     LADAGWLGICIPEEYGGSGLGITDAAIMMRTISESGAGMSGASAVHMNVFGLNPVVVF
                     GTDEQRRRMLPPIVAGTEKSCFAVTEPNTGLNTTQLKTRAERKGDKYLVNGQKVWIST
                     AQVANKMLLLARTTPLEVVKNPTRGLSLFYTDFDRTRVTVHEIEKMGRKAVDSNELFF
                     ENFEIPVEDRLGEEGRGFEYILHGMNPERILIAAEAVGLGKVALSRAADYAKSRIVFN
                     RPIGKNQAIQHPLAKNWMELEAAWLMTL"
BASE COUNT          539 a          833 c          876 g          504 t
ORIGIN      
        1 ggcgctgctt gcggacggtg aagtcgtgtg gtcctgacgc ctcgacgctg gcgtcaagtc
       61 ttgcggaggc tcgttcggct caaccgggtc ggacaaaacc ttcaatccgc agggcgacgg
      121 tggcaaaaga gcccgatcgc ccgggagagt acgaagtaaa ccgttaaaac cattgcgtgc
      181 gggaatgccg ggtgatttcg gtgtgctcgt tgttactcgt gtgcttttta ccactaccaa
      241 atgcacacga gaccgcgggt gcatcgtgcg cccggcattc cccacgccct ctgatgggcg
      301 agaactgatg cagaggctcg ggcgcgcatt gcgccgcgag gcgtgaacgc gtgttgagat
      361 cgaggcacga acctctcagc cagtcgtccc ggcgaacgcc gggatccata accaccggat
      421 gtcgttgttg tgccgtggcg tcagcgtcgt ctttcaaatt tcagcgtcac ggagtatggg
      481 tcccggcgtt cgccgggacg acgcggagtg tggggcgcgc atacgcccat acgcctgacg
      541 cccttgatct tcgcgatggg ctgccgttac taggtccaag aagtaatatc gcggcgggaa
      601 cgccgtatat ccgggagcaa accgtaccat gggaccattg cagggcatca aggtcgtgga
      661 catgacgacc gtgctgaagg ggccctatgc gacccagatg ctcggcgact atggcgccga
      721 tgtcatcaag atcgaaacaa cagacggcga cgtgacgcgg cagatcggcc cgacgcgcca
      781 tcccggcatg gggccggtgt tcctcaatac caaccgcagc aagcgcagcg tctgcctcga
      841 cctcaagaaa gccgcggggc gcgacgcggt attgcggctg atcgcgtcgg ccgacgtgct
      901 ggtctataac gtgcgtccgc aggcgatggt gcggctgcag ctcggctacg acgtggtatc
      961 caagatcaat ccgcggctga tctatgccgg cgtgttcggt ttcggccagg acgggcccta
     1021 tgcggcaaaa cccgcctatg acgacctgat ccagggcgcc accgcgctgc ccgccttgat
     1081 ggcgcagacc aacgacggcg ttccgcgcta tgtccccaac gcgctggtcg atcggatcgt
     1141 cgggctcacg gccgtcggtg cgatctgcgc cagcctggtt catcgcgagc gctccggacg
     1201 cggtcagcgc gtcgatatcc cgatgttcga gaccatggcg gggtttgtca tgggcgatca
     1261 catgggcggc ctgacctacg agccgccgct ggacagaggc ggctatgcgc ggcatctggc
     1321 gcgtgaccgc aggccgtata agacgttgga cggttatatc tgcgtgatcg tctacaacga
     1381 caagcaatgg gaaaacttct tcaaggccac cggccgcgac gatctgcgca gcgatcccaa
     1441 gttcgcgacc tttgccgggc gcgccgtcaa tattgatacg gtctacgggg aattggcccg
     1501 catcttcgaa acacgaacca ccgccgaatg gaccagcctg ctcgaaaacg cggacgtgcc
     1561 tgtgatgccg atgcatgatc ttgagagcat cctgcaggac ccgcatctgg tggcgacgga
     1621 tttcttttcg gtcgttgttc atccgaccga ggggccgatt cgaaacatga aggtggccgc
     1681 cacctggtcg gagacatttg ccgacccgac gcggctggcg ccacggctta accagcacgg
     1741 tgccgagatt ttgcgcgaag ccggcttctc caccgacgag atcgcaacgc tcgagcgcga
     1801 cggtgtcacc cgagcgccgc acagcccgca ggactgatcc catggatttt gccctcaccg
     1861 ccaaccagga atcgatccgc gacgccgtgg caaggatctg cgcgcgcttc gacgatgcct
     1921 attggctgaa gaaggacaag gagggcggtt tcccggccga cttccacaag gcgctggcag
     1981 atgccggctg gctcggcatc tgcatccccg aggaatatgg cggctcgggc ctcggcatca
     2041 ccgatgccgc gatcatgatg cggacgattt ccgaatccgg cgctggcatg tccggggcgt
     2101 cagccgtgca catgaatgtg ttcgggctta atccggtggt ggtgttcggc accgacgagc
     2161 agcggagaag gatgctgccg ccgatcgtcg ccggcaccga aaaatcctgc tttgccgtca
     2221 ccgaaccgaa cacggggttg aatacgacgc agttgaagac ccgcgcggag cgaaagggcg
     2281 acaaatacct cgtcaacggc cagaaggtct ggatctccac cgctcaggtc gccaacaaga
     2341 tgttgctgct ggcccgtacc acgccgctgg aagtggtgaa gaatccgacc cgcggcctca
     2401 gcctgttcta taccgatttt gatcgaacgc gcgtcacggt ccacgagatc gagaagatgg
     2461 gccgcaaggc cgtcgactcc aacgagctgt tttttgagaa ttttgaaatc ccggtggaag
     2521 atcgtcttgg cgaggaaggc cgcggtttcg aatacattct ccatggcatg aatccggagc
     2581 gcattctcat cgccgctgaa gccgtcgggc tcggcaaggt ggcgctgtcg cgtgccgcgg
     2641 actacgccaa aagccgcatc gtgttcaatc gtccgatcgg caagaaccag gcgatccagc
     2701 atccgctggc gaagaactgg atggagctcg aagccgcgtg gctgatgacg ct
//
LOCUS       DASWGM010000003         6106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_831859,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000003 DASWGM010000000
VERSION     DASWGM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831859"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(412..1005)
                     /locus_tag="VGA15_00045"
     CDS             complement(412..1005)
                     /locus_tag="VGA15_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEX9586121.1"
                     /translation="MKRAQKTALKKAGGTAKSKNQPAVVAERPGSWHLARTETERRLC
                     DFEFGLERLAQAYYRWKAACLAAVCDVPLTGDDVAVLNVVRMGDEPKRLSEIGQLLNR
                     VDVPNLQYATRKLVRSGLIETEGSSSRKETRYRATATGHSVTEAYATLRAATLPPMLD
                     ALDGWAAKSETTSIQLDLISSLYAQAAQVAITRRHQP"
     gene            1185..2426
                     /locus_tag="VGA15_00050"
     CDS             1185..2426
                     /locus_tag="VGA15_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9586122.1"
                     /translation="MAGQWIDVAAADGGTFKGYLTIPASGSGPGILLLQEIFGVNTSM
                     REVADYYAEEGYVVLAPDLFWRLEPGIELGYSEADFNKAFGYYQRFDADQSIKDAADA
                     LKVLRARPECAAKVGALGFCLGGKLAYLTAARTDVDCAVSYYGVGIEADLGEVAQIKG
                     PMVFHFAELDKFAPAEAREQVKVAFAKRPDVELYLYPGCDHAFAAPERASFNKPATLM
                     AHSRSIALFRKVLGPRYDLSTLWDRHTELEFGVRSAEATMGTMVAEPYVNHIPTMTGG
                     VGYRDLLRFYANHFIPKTPKDTKLIPISRTIGADRVVDEMLFCFTHDIEIDWMLPGVP
                     PTGKYVEIPLVAIVRFRGDKLYNEHIYWDQASVLVQIGLLDSALLPVAGVATAKKLVD
                     ETLPSNTLMARWAESAPQAAE"
     gene            2485..4029
                     /locus_tag="VGA15_00055"
     CDS             2485..4029
                     /locus_tag="VGA15_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586123.1"
                     /translation="MVSSVLSRRHVLRLAGAAALPLSSGLLGGPAQAAAAGTLTIAYN
                     VNLPSFDPTIGLSAVNPTLQSIYQAIFDPYIGQAPDLSFKPGLLTKWGWNADHTKITL
                     ELRKGVTWHDGSPVTPEDVVWSLQRAADPKSGNPIQFVWSKVGNYKIDGNIITGDVIE
                     FEPTLFKWMAFLTGYILPKAYYEKVGAEGFEKAPIGSGPYKVDAFERNAFLRLKTHPT
                     YWGPKPAFDTVVFKFVTDPTSRVAEMESGGSDVTFEIPYEEFDRLKAKAGLAGQTKPV
                     SDIAMIFITDIAPMLDRNVRLAANHAIDKKAIVDRLLKGYGVPIATLEAPGYAAFDPS
                     IKVSYDPELAKSLLAKSGFSPEKPVKFTIQTTRGFKPKDYEMIQAIVGMWRKVGIEAN
                     IEVYEIAQHFELRARHALAPAAFYNWGNAIGDPSTSTGFAMFGPSPHCAWKGKDLVER
                     IGPLWGEKDEAKRIAGWKAVDKYIAEEGEVIPLFQYVQPIIHKKGLKVVAQANGMILP
                     QLITQA"
     gene            4055..5062
                     /locus_tag="VGA15_00060"
     CDS             4055..5062
                     /locus_tag="VGA15_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586124.1"
                     /translation="MRWGEIIRRLVLIPPTLVGVAVIVFVLLRVVPGDPIAMMIPPGA
                     SPADIDRLRAFYGLDQPILQQFITWFGQALRGDFGRSISVHQSVFELVLARLPATLEL
                     AALATLIAVVLGTIAAMIGIQLRGRRAEWVVDGGVGVFLAIPDFLWALILLLLFGVLI
                     PLLPISGRIDPQIDVNFHSNFYLVESFLTGRFDIANALLHHMLLPALALALPFAALVA
                     RILKTSLAEAEDQDYAQIARARGFSRPAILLREVFPNALIPTVALGGVQITLLLGGTV
                     LVERIFSYEGIGNMAIDAVINRDFPLIQGLILTFAVLFIALNLGVDLVVTLLDPRLRH
                     G"
     gene            5055..5942
                     /locus_tag="VGA15_00065"
     CDS             5055..5942
                     /locus_tag="VGA15_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586125.1"
                     /translation="MVDLALDSALGPPSRWIAGLKRGGPRLWVGAALVGVLVLAALLA
                     PLIALHDPLEQDLMSAQLPPAWVVGGDLAYWLGTDSLGRCVLSRLIYSARTAVAVALI
                     AASLAAAIGIAFGLIAGSFGGWVDQATSRLIDVWMAFPPVLLSIVLAAVIGAGLTSVI
                     AAIVVVDWTRFARVVRAETMVQLQRDYAAAARVIGLSRSGILRLEILPNLIPLVVTLL
                     AVEMGIAILVEVILSFVGISVAGDTPTWGGMIAEGRQIVYQAPWIMGLPIGCIISSVI
                     GFNLLGDGLRLVLDPVQRR"
     gene            5939..>6106
                     /locus_tag="VGA15_00070"
     CDS             5939..>6106
                     /locus_tag="VGA15_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9586126.1"
                     /translation="MTAALSVQGLQVAIGTGASSANVLRGVSLDLQRGEVRGLVGESG
                     AGKSMLGRAVLG"
BASE COUNT         1064 a         1863 c         1914 g         1265 t
ORIGIN      
        1 actcgatcgc cattgcaccg aacatatcgc gcggttcaaa cggccgaaac gctatgtgtt
       61 cgtcgaggag ctaccgaaga acaattacgg caaggcactc aaacgcgaat tgcgggaaca
      121 attgccgcct gtgtgtaccg aaaaccctat ccgggtttgt tgaacgaaga atcggctaag
      181 ttggcgcggc cggtgcgtgt gccgttgaat gggaccgttg gccaacccgg gcgcatcgag
      241 gcggttgaga atttcgctgc cgatgcaaag gccaagggta cacgtcattc tgacgggcgg
      301 gggtcgtatc agaaaccgcg gctatttttt ccctctgacg gttcttgcag aagtgctcga
      361 cgacgcgcgc gtcatccccc ggcccaatag ccgcagcccg ctgatgcgct ttcagggctg
      421 atgccgtcgg gtgattgcga cctgcgccgc ttgagcgtag aggctcgaga tcaaatccag
      481 ttggatgctc gttgtctcgg acttcgctgc ccaaccgtcc agtgcgtcca gcatcggcgg
      541 caacgtcgcg gcgcgcagag tggcgtaggc ttcagtgacg gagtgccccg tcgcggtggc
      601 gcggtagcga gtttccttgc gcgatgagct gccctcggtc tcgatcaagc ccgaacgaac
      661 aagcttgcgc gtggcgtatt gaagattggg gacatcgacg cggttgagca actgcccgat
      721 ctccgaaagc cgcttcggct catcgcccat ccgaaccaca ttgagcacgg cgacatcgtc
      781 gcccgtgagc ggcacatcgc agacggcggc cagacacgcc gccttccagc gatagtaagc
      841 ctgcgccagt cgctccagcc cgaactcgaa atcgcacagc cgccgctcgg tttcggtgcg
      901 cgcgagatgc caggaccctg gccgttcggc gacgacagca ggttgatttt tcgacttcgc
      961 ggtaccgccg gctttcttca gcgcggtctt ttgggccctc ttcaaaacgg tctcctgatg
     1021 tcgcgtgcgc tcaaggcata ggcaagccgt gttgaatcta tcctatttac aattacgatt
     1081 cttatattaa aaattcaatt aagaatacac gatgaagtgg caataccgct gcaagcggcg
     1141 gtgccaggcg ttggcgctga gcgtttcgga cggaggtcga tacaatggcg ggacaatgga
     1201 tcgacgtcgc ggccgccgat ggtggcacct tcaagggata tctgaccatt ccggcatcgg
     1261 gatcgggtcc cggtatcctg ctgctgcagg agatttttgg cgtcaatacc tcgatgcggg
     1321 aggtcgccga ttattatgct gaggaggggt atgtcgtgct tgcgccggac ctgttctggc
     1381 gattggagcc tggcattgag ctcggctatt ctgaggccga cttcaacaag gcgtttggct
     1441 attaccagcg cttcgacgcc gatcagtcga tcaaggacgc cgccgatgcg ctaaaagtgc
     1501 tgcgcgcgcg tcccgaatgc gccgccaagg tcggcgcgct cggcttttgc ctcggcggca
     1561 agcttgccta tctgaccgcg gcacgcaccg atgtcgattg cgccgtctct tattacggcg
     1621 tcggcatcga ggccgacctt ggcgaggtcg cgcaaatcaa aggcccgatg gtgtttcact
     1681 ttgccgaact cgacaagttt gcgccagcgg aagcgcgcga acaggtcaag gtcgccttcg
     1741 ccaagcgtcc cgatgttgaa ctgtatttgt atcccggctg cgaccacgcc ttcgcggcgc
     1801 cggagcgggc gagcttcaac aagccggcga ccctgatggc gcactcgcgc tcgatcgcgc
     1861 tgttccgaaa agtactgggt ccgcgttacg atctctccac gctatgggat cgccataccg
     1921 aattggaatt cggcgtgcgc tcggccgaag cgacgatggg aacgatggtg gcggagccct
     1981 atgtcaatca cattccgacc atgaccggcg gcgtcggcta tcgtgatctc ctgcgctttt
     2041 acgccaatca tttcattccg aagacgccga aggataccaa gctgatcccg atctcgcgca
     2101 ccatcggtgc cgatcgcgtc gtcgacgaga tgctgttctg cttcacccac gatatcgaga
     2161 tcgactggat gctgcccggc gtgccgccga ccggtaagta tgtggaaatt ccgctggtcg
     2221 cgatcgtccg cttccgcggc gacaagctct acaacgaaca catctactgg gatcaggctt
     2281 cggtgctggt gcagatcggg ctactcgatt ccgctctgct ccccgtggcc ggtgtagcga
     2341 cggcgaagaa actcgtcgac gagacgctac cgtcgaacac gctgatggcg cgctgggcag
     2401 aaagtgcgcc gcaggccgcc gaatagtcgc attcagtgta tggcgcccgc tccgggcgcc
     2461 attctcgcat ctggagtaat tgtcatggtg tcctccgtgc tttctcgccg ccacgttctt
     2521 cggctggccg gcgccgccgc cctgccgctc tcctccggtc tcctcggtgg ccccgcgcag
     2581 gccgcagccg caggaacgct gaccatcgcc tacaatgtca atttgccgtc gttcgatccg
     2641 accattggat tgtccgcggt caatccgacc ctgcagtcga tctatcaggc gatcttcgat
     2701 ccttacatcg gtcaggcgcc cgacctttcc ttcaagcctg gtctcttgac gaaatggggc
     2761 tggaacgccg accacaccaa gatcacgctg gagctgcgca agggcgtgac ctggcacgac
     2821 ggctcgccgg tgacgcccga agacgtggtc tggtcgctgc agcgcgcagc cgatccgaaa
     2881 agcggcaatc cgattcagtt cgtctggtcc aaggtcggca attacaagat cgacggcaac
     2941 atcatcaccg gcgacgtcat tgaattcgaa ccgaccttgt tcaaatggat ggcttttctc
     3001 accggctata ttctgccgaa ggcctattac gagaaggtcg gtgcggaagg ttttgagaag
     3061 gcgccgattg gctccggtcc ctacaaggtc gatgccttcg aacgcaatgc cttcctgcgg
     3121 ctcaagacgc atccgaccta ttggggacct aagcctgcct tcgacaccgt ggtgttcaaa
     3181 ttcgtcaccg atcccaccag ccgcgtcgcc gagatggaat cgggcgggtc ggacgtcacg
     3241 ttcgaaattc cctacgagga attcgaccgc ctgaaggcga aagcggggct ggccggacaa
     3301 accaagccgg tgtccgacat cgcgatgatc ttcatcaccg acatcgcgcc gatgctcgac
     3361 cgcaacgtcc gtctcgccgc caatcacgcg atcgacaaga aggcgatcgt cgaccggctg
     3421 ctgaaggggt acggcgttcc gatcgcgacg ctggaagcgc cgggctacgc cgccttcgat
     3481 ccctcgatca aggtgtctta cgatcccgaa ctggcaaaaa gcctgcttgc caagagcggc
     3541 ttctcgccgg agaagccggt caagttcacg atccagacga cgcgcgggtt caagccgaag
     3601 gattacgaga tgatccaggc gatcgtcggg atgtggcgca aggtcgggat cgaagccaat
     3661 atcgaggtct acgaaatcgc gcagcatttc gagctgcgcg cgcgccacgc gctggcgccg
     3721 gcggcgttct acaattgggg caacgcgatc ggcgatccct ccacctccac cggctttgcg
     3781 atgttcggcc cgtcgccgca ttgcgcctgg aagggcaagg atctcgtcga gcgcatcggc
     3841 ccgctgtggg gcgaaaagga cgaagccaag cggatcgccg gctggaaggc cgtcgacaaa
     3901 tacatcgcgg aggagggcga ggtgatcccg ctgttccagt atgtgcagcc gatcatccac
     3961 aaaaagggcc tgaaggtcgt ggcgcaggcc aatggtatga ttcttccgca actgatcaca
     4021 caggcttagg gacgcaagat agggcgacag cacgatgcga tggggcgaga tcatcaggcg
     4081 gctcgtcctc ataccgccca cgctggtcgg cgtggcggtg atcgtcttcg tgttgctgcg
     4141 cgtggtgccc ggcgacccca tcgccatgat gatcccgccg ggcgcttcgc ccgccgacat
     4201 cgaccggctg cgggcgtttt atggacttga tcagcccatc ctgcagcagt tcatcacctg
     4261 gttcggccaa gcgctgcgcg gcgatttcgg acgttcaatc agcgtgcacc agagtgtgtt
     4321 cgaactggtg ctggcgcgat tgccggctac gctcgaattg gcggcgctgg cgacgctgat
     4381 cgcggtggtg ctgggaacga tcgccgcgat gatcggcatc cagcttcgcg gccggcgggc
     4441 ggaatgggtg gtcgacggtg gcgtcggtgt cttcctggcc attccggatt ttctctgggc
     4501 cctgatcctg ctgctgctgt tcggggtgct gatcccgctc ttgccgattt cgggccgaat
     4561 cgacccgcag atcgacgtca acttccacag caatttctat ctggtcgaaa gctttttgac
     4621 cgggcgcttc gacatcgcca acgcgctgct gcatcacatg ttattgccgg cgctggcgct
     4681 ggctctacca tttgcggcat tagtggcgcg gattttgaaa acctcgctgg cggaggccga
     4741 ggatcaggat tacgcgcaga tcgcccgcgc ccgcggtttt tcgcgtccgg cgatcctgct
     4801 gcgcgaggta tttcccaacg ctctgattcc gacggttgca ctcggcgggg tccagatcac
     4861 gctgttgctc ggcggcaccg tgctggtcga gcgcatcttc tcctatgagg gaatcggcaa
     4921 catggcgatc gacgcggtga tcaatcgcga ttttccgctg atccagggcc tgatcctgac
     4981 cttcgccgtt ctcttcatcg cgctcaatct cggcgtcgat ctcgtggtca cgctgctcga
     5041 tccgaggttg cgccatggtt gaccttgccc tcgatagcgc gctcgggcct ccgtcgcgct
     5101 ggatcgccgg attaaagcgg ggcgggcctc ggctctgggt cggtgcggcg cttgtcggcg
     5161 tcctcgtact ggccgcgctg ctggcgccgc taatcgcgct gcacgatcca ctcgaacagg
     5221 atctgatgtc ggcgcaattg ccgccggcct gggtcgtcgg cggtgacctc gcgtattggc
     5281 tcggcaccga cagtcttggc cgctgcgtgt tgtcgcgctt gatttactcg gcgcggaccg
     5341 ccgttgcggt cgcgctgatc gcggcatcgc tggcagccgc aatcggcatc gcctttggcc
     5401 tgatcgccgg cagtttcggc ggctgggtcg atcaagccac ttcgcgcctg atcgatgtct
     5461 ggatggcatt tccgccggtg ttgctgtcga tcgtgctggc ggcggtgatc ggcgccgggt
     5521 tgacctcggt gatcgctgcc atcgtggtcg tcgactggac ccgctttgcg cgcgtggtcc
     5581 gggccgaaac catggtgcaa ctgcagcgcg attacgccgc tgccgcgcgc gtcattggac
     5641 tgagccgcag cggaatcctg cggctggaga ttctgcccaa cctcattccg ctggtggtga
     5701 cgttgctggc ggtcgagatg ggcatcgcga tcctggtgga agtgatcctg tcttttgttg
     5761 gcatttcggt ggccggcgac accccgacct ggggcggcat gatcgccgag ggacgccaga
     5821 tcgtctatca ggcgccatgg atcatggggc tgccgatcgg ctgcatcata tctagcgtga
     5881 tcgggttcaa tctgctcggc gacggcctgc gtttggtgct cgatccggtg cagcgacgat
     5941 gacagcggcg ctttccgtcc agggtctgca ggtcgcgatt ggcacgggcg caagctcggc
     6001 caacgtgctg cgtggcgtta gcctggacct gcagcgcggc gaagtccgcg gattggtcgg
     6061 ggaatccggc gccggcaaat cgatgctggg acgtgccgtc ctcggc
//
LOCUS       DASWGM010000004         7045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2356820,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000004 DASWGM010000000
VERSION     DASWGM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2356820"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VGA15_00075"
     CDS             <1..291
                     /locus_tag="VGA15_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEX9586127.1"
                     /translation="RTKGRKIDPSLGFERRWVVYNGYAEATRVPPSWHGWLHHTVDVA
                     PTEETYKRYEWEKPHIPNLTGTPLAYRPSGSTLASGRRPAATGDYQPWTPGN"
     gene            complement(822..1697)
                     /locus_tag="VGA15_00080"
     CDS             complement(822..1697)
                     /locus_tag="VGA15_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586128.1"
                     /translation="MASFVVEPHFRLQEWVAQEKGYFRDQGLDYEFRELIRSTEGQHH
                     NKGGRGAFQSIEQGREADVSCACHWTVNVAASTGHTKLYADLYSVAPSGIFVPADSNV
                     RTPADLAHVPISVGFQSGSHYSTIQALEQYMPPGEINLIFQDGMLFSRMERFIDGKSP
                     AAALFNGPYYLAEQLGFRKVIDTTFIIAAMIKGSPDPEDIRRYFRALRSAQRDIDLRP
                     ERYTHYYKNEFPDRFHAMMDTRRWGPGERIVFEPYSKEVFEESFDWIAKHKIFSDGAM
                     GSGDYDRSTISFAAE"
     gene            1794..2777
                     /locus_tag="VGA15_00085"
     CDS             1794..2777
                     /locus_tag="VGA15_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9586129.1"
                     /translation="MDQKAYSELSLRELRVLNALLALRSITRTAEAMETTQPAISKML
                     RHLRVQFSDPLFVRNGQAMQPTAKALDIAHRLRVLLAAADGLRSAATEFDPASSDRPF
                     SLLLTDVGMIRFLPPLIARVAAIAPGISVRAVPLDARQFEYRLETGEADLAFGAFPKA
                     ARHLRRQRLYFDGYSSVLRKRHPRASKSQSRIGFLAERHILVTGSETGHAAHLTAHRV
                     LSSAIAPGNVMLRVPSFIAGAIVAAETDGVATLPTNLAQRLAGPLGLVAFETPIALPR
                     IEIAQFWHERYHRDAGHQWLRNVTFELFARSAKGTSLKNFSEKAAKSDKRG"
     gene            complement(3065..4216)
                     /locus_tag="VGA15_00090"
     CDS             complement(3065..4216)
                     /locus_tag="VGA15_00090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9586130.1"
                     /translation="MLTQSVTSELKQQTTDVADRLLHHCPTFLDGTEPDKSLVSAALL
                     EFANAGLFDLGYLDPASNGISADDMRRATIIVSALAGRSLSLATIYMVNAVFGGAFVA
                     QIATTGQKRDLLPAIRNGTAQIAFAMTEPQAGSDAAALGTQAKAVGQGFAISGEKIYI
                     TGAASADHVLVVARFEGGDKRAISLFLVPTGAQGLVIKPLPRIAGNTHGSCQLFLDDV
                     RVGPDQLLGGNEKLGKAWATLRFTGSLERIVVAATATGLAGAIVDRAIDFAKERRQFG
                     NTIASFQAVQHMLVEMRTAETGMRLFVDHAVDMLASGESDIAASMAKFHCAEQLQEIV
                     AKGMRIMGGRACFTFEQMSRFYCEAPFSLYAGGTIEIQKALIARAMGIG"
     gene            complement(4253..4960)
                     /locus_tag="VGA15_00095"
     CDS             complement(4253..4960)
                     /locus_tag="VGA15_00095"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX9586131.1"
                     /translation="MSAPLQIGLVLFPKVTQLDFTGPLQVFSSVPGANVHLIWKRIEP
                     VASDSVMMLTPTTTFADCPQLDVICVPGGFGTDDMVNDEEMLDFLRRQAKGAKYITSV
                     CTGSLVLGAAGLLQGYRATTHWTAMENLAQFGATPTKTRVCVDRNRVTGGGVTAGIDF
                     ALTLVSLMVDRQGAEMVQLRLEYNPAPPFNSGSPDTAPPEVLALMKARIAPGQARRTE
                     ATNRAAARLATLHPSTA"
     gene            5191..6129
                     /locus_tag="VGA15_00100"
     CDS             5191..6129
                     /locus_tag="VGA15_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX9586132.1"
                     /translation="MIGVVVFPDFQLLDAAGPISVFEIAARFAGQPPSIKVLAVTSGP
                     VRSSSGVEMLARGLRPSGAITTLIVAGGEGVRTAATCEKTLGFVRTMVKRGIRVASVC
                     SGAYILAEAGVLDGRRATTHWQRTRHFVSAYPKIKLEADRIFVRDGNIWSSAGISAGI
                     DLALALVAEDFGEEIAQKTARQLVLYHRRGGGQSQFSSLLELKAPAGRFGPLLAWARE
                     HLDAPLTVEDMADQAGMSSRHFARAFIAETGTTPSKAVERLRIEVARQRVQSSSEAIE
                     RVAQVTGFRDPERMRRAFIRAFGQPPQSLRRAARAG"
     gene            6271..6666
                     /locus_tag="VGA15_00105"
     CDS             6271..6666
                     /locus_tag="VGA15_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEX9586133.1"
                     /translation="MSTNATTLARTIMAMRPMVPAKDFEISKRFYIELGFEPKILTDR
                     LVEMQLGAFSFILQAYYVRQWADNFVMHMRVSDVKLWWDHIVGLDLASRYGVKTRAPQ
                     MEDWGLVAAVIDPSGVLWRIAEASAPHPN"
     gene            complement(6694..7044)
                     /locus_tag="VGA15_00110"
     CDS             complement(6694..7044)
                     /locus_tag="VGA15_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HEX9586134.1"
                     /translation="MRYYERVEMLAPPPRTAGGRRVYDTTDLRTLVFIRRSRELGFSL
                     GEIRALLRLGGPGKASCREVREIAARHLEDIRAKLSDLKKLERVLSKTLARCSGETAP
                     DCPVLDILDIQRAS"
BASE COUNT         1368 a         2149 c         2167 g         1361 t
ORIGIN      
        1 cgcaccaagg gccgcaagat cgatccctcg ctcggcttcg agcggcgctg ggtggtgtac
       61 aacggttatg ccgaagccac gcgggtgccg ccgtcgtggc atggctggct gcatcacact
      121 gtggatgtcg cgccgaccga agagacctac aagcggtatg aatgggaaaa gccgcatatc
      181 cccaacctga cggggacgcc tttggcctac cggccctccg gctcgacgct ggcaagcggc
      241 cgccggcccg cggccaccgg cgactaccag ccgtggacgc cgggcaactg agttttacga
      301 ccatcgtcag aacttcaacc gtcgtcatag tactgactgt cgtcattccg gggcgcgcga
      361 agcgcgagcc cggaatccat aaccacgacg gggggtatgg attccgggcc tgcgccaagc
      421 ggcgcatccc ggaatgacga agtgaattat ccggaagccg gtagtgtctc gagacactac
      481 ccggaagccg cgcgaccgcc tgttaacaac ggggacagcg gggacatctt tcgctgcgtc
      541 cttgagaacc ggacgcgatc gtggctcaat caatcatctt acggagatgg gagatcatcg
      601 cgtcggttcg ggccacagtt cgcgactgcc ccgatttgca gcggcacccg atcaggatac
      661 ggctgggaga taggcccccg gcgcagaggt cctgaaatcg cccgacaatg tggagctgcc
      721 gtaggacagg aaaggccgtc tgggagacca ggcggccttt ttcttgtgcg gctttctttg
      781 cggcgtcgcg cccgcctcgc gcgaagcgga cggctgccgc gctactccgc ggcaaaagag
      841 atcgtcgacc gatcatagtc gcccgatccc attgctccat cggagaagat cttgtgcttt
      901 gcgatccagt cgaatgattc ctcgaaaacc tctttcgagt agggttcgaa tacaattctt
      961 tctcccggcc cccagcgccg cgtatccatc atcgcatgga agcggtcggg gaattcattc
     1021 ttgtagtagt gagtgtagcg ttccgggcgc aggtcgatgt cgcgttgcgc cgagcggagc
     1081 gcgcggaagt agcggcgaat gtcttccggg tccggcgagc ccttgatcat ggcggcgatg
     1141 atgaaggtgg tgtcgatcac cttgcggaag ccgagctgtt cggcgaggta ataggggccg
     1201 ttgaagagcg ccgcggccgg gctcttgccg tcgatgaagc gctccatgcg gctgaacagc
     1261 atgccgtctt ggaaaatcag gttgatctcg ccaggcggca tgtactgttc cagcgcctga
     1321 attgttgaat agtggctgcc ggactggaat ccgaccgaga tcggcacatg ggccaggtcg
     1381 gccggcgtac gcacgttcga atcggcggga acgaaaatcc ctgacggagc gaccgagtac
     1441 aggtcggcat acagtttggt gtggccggtc gaggctgcaa cgttcaccgt ccaatggcag
     1501 gcgcagctga cgtcggcctc gcggccctgc tcgatactct ggaaagcgcc ccggccgccc
     1561 ttgttatggt gctggccttc ggtcgagcgg atcaattcgc ggaattcgta gtccagtccc
     1621 tgatcgcgaa aataaccctt ctcttgtgca acccattcct gaagccggaa atgcggctcg
     1681 acgacgaagc tggccatgga gatcctccgc tgtcgcccgg aattggcgat tgttattgac
     1741 gcaagctatc tctcgaccaa gacttgcgcc atgccaaaac gggaatgatg aatatggatc
     1801 agaaagcata ttcggagttg agcctgcgcg agctgcgcgt tctcaatgca ttgcttgcgc
     1861 tgcgcagcat cacccgaaca gcggaggcga tggagacgac gcagccggcc atcagcaaga
     1921 tgctccggca cctgcgcgtg cagttttcgg atccgctgtt cgtgcgcaac ggccaggcca
     1981 tgcagccgac ggccaaggcc ctcgacatcg cccatcggct gcgcgttctt ctcgctgccg
     2041 ccgacggctt gcgttcggcg gcaaccgagt tcgatcctgc cagttcggat cgtccgttca
     2101 gcctgctttt gaccgatgtc ggaatgatca ggtttctgcc gcccttgatc gcgcgcgttg
     2161 cggcaatcgc gcctggcatc agcgtgcgcg cggtgccgct ggatgcgcgg cagttcgagt
     2221 acaggctcga aaccggcgag gccgatctgg cgttcggcgc gtttccgaag gccgcccgcc
     2281 atctgcggcg gcagcgcctg tatttcgacg gttattccag cgtgcttcgc aaacgccacc
     2341 cgcgcgcatc gaaatcccaa tcacgcatcg gctttctcgc cgaacgccac attctggtaa
     2401 cgggatcgga gaccggacac gccgcgcacc ttaccgcgca ccgcgtgctc tcttctgcca
     2461 ttgcgcctgg gaatgtcatg ttgcgagtgc cgagctttat tgccggcgcc attgtcgcgg
     2521 cggaaaccga cggcgttgca accctgccga ccaatttggc gcagcgcctc gccgggccgc
     2581 tcggtctcgt cgcgttcgaa accccgatcg cgctgccacg gatcgaaatc gcccaattct
     2641 ggcatgaacg ttaccaccgt gacgccggcc atcaatggct gaggaacgtc acgttcgagt
     2701 tgttcgcccg ttcggctaag ggtacgagcc tgaagaattt ctctgagaag gctgctaaga
     2761 gcgacaaacg tggctgagtg ccggtagatg aaacatcggc tcgtgcctga tcgcggctcg
     2821 agaactcagg gcgaggtagc taccgcctac tcaaccacgc gcaatgcccc cgacaaaaca
     2881 cggtatgcgg gatcgacggc atttgacggt catcggctgc aatttcccaa gaacgaaaac
     2941 gaaagaatag tagaacatgc gcgcacagga tttgcacaca actcctgtct agccgccgca
     3001 attcaccgca cggtcgcaac atttgaccgg aacgagagaa cccttggtat ttattattcg
     3061 ggggtcatcc gattcccata gctctggcga tcagtgcttt ctggatctcg atggtcccgc
     3121 cggcgtagag actgaatggc gcttcgcagt agaagcgtga catttgttcg aacgtgaagc
     3181 aggcgcgtcc gcccataatc cgcatgccct tcgccacgat ctcctgcagt tgttcggcac
     3241 aatgaaattt tgccatggag gctgcaatgt ccgactctcc ggacgcaagc atatccacgg
     3301 catgatcgac aaacaaccgc atgccggttt cagcggtccg catctcgacc agcatatgct
     3361 gtacagcctg aaacgaggct atcgtgtttc cgaattggcg ccgctccttg gcaaaatcga
     3421 tggcccggtc gacgatggcg ccagccaggc ctgtagcggt cgctgccacc acgatgcgct
     3481 ccagcgaacc cgtgaagcgc aacgttgccc acgctttgcc caacttctcg tttcctccca
     3541 acagctgatc aggaccgacg cggacatcgt caagaaacaa ttgacatgag ccgtgtgtat
     3601 tacccgcaat acggggcaag ggtttgatca ccaagccttg cgccccggtg ggaacaagaa
     3661 agaggctgat ggcgcgcttg tcaccgcctt cgaagcgcgc cacgacaagc acgtggtcgg
     3721 cactggcagc tccggtgatg tagatcttct cgccgctaat cgcaaacccc tggccaacgg
     3781 cctttgcctg agtaccaagt gcggccgcgt ccgaacctgc ctgcggttcg gtcattgcaa
     3841 acgcaatttg cgcggtccca ttgcgaatag cgggaagcaa gtcgcgcttc tgtccggtcg
     3901 ttgcaatctg agccacgaac gcgccaccaa acactgcgtt gaccatataa atcgtggcta
     3961 aagataatga tctgccggcc agcgcgctga cgatgatcgt ggcccgacgc atatcgtctg
     4021 ccgaaatgcc gttggaggcc ggatcaaggt atccgagatc gaacaatcca gcgttagcga
     4081 attccagcaa cgccgcggac acaagcgact tgtcgggctc cgtgccgtcg agaaacgtcg
     4141 gacagtgatg caggagacga tctgcaacgt ccgtagtttg ctgcttcagc tcgctggtaa
     4201 ccgattgtgt caacatgagt gccgtgttcc ttgccggtag tcggagcggc gctcacgccg
     4261 tcgagggatg cagcgtggca agccgcgccg cggcgcggtt ggtcgcctcg gtgcggcgtg
     4321 cttgcccggg cgcgatccgc gctttcatca gcgccagcac ttccggcggc gcggtatcgg
     4381 gcgagcccga gttgaacggc ggcgccgggt tgtattcgag ccggagctga accatctccg
     4441 cgccctgccg gtcgaccatc agcgacacca gcgtaagcgc gaaatcgatg ccagcggtga
     4501 cgccgccgcc ggtgacgcgg ttgcggtcga cgcagacccg ggtcttggtc ggcgtcgcgc
     4561 cgaactgcgc gaggttttcc atcgccgtcc aatgcgtcgt cgcgcgataa ccttgcagca
     4621 ggcccgccgc gcccagcacc agcgatcccg tgcagaccga agtgatgtac ttggcgccct
     4681 tggcctgcct ccgcaaaaag tccagcatct cttcgtcgtt gaccatgtca tcggtgccga
     4741 acccaccggg aacgcagatc acatcgagtt gcgggcagtc cgcaaaggtt gtggtcggcg
     4801 tcagcatcat cacggagtcg ctggcgaccg gctcgatgcg cttccagatc agatgcacgt
     4861 tggcgccggg cacgctggag aacacctgta gcggcccggt gaagtcgagt tgcgtcactt
     4921 ttggaaacag cacgagacca atctgaagcg gcgcggacat gggtagagcc tttcgaggtt
     4981 tgcgtttcta gcgacaattc gtcattgccg ggcttgaccc ggcaatccat ccgcttccca
     5041 agactctttc gaagaaggat ggatgcccgg atcaagtccg ggcatgacga ccgaacgtgg
     5101 ggaggtcgac ccgagacttg acgcggccat cctgtcatga tcgtcttctg tccgaaatgc
     5161 catatttccc tcatttcagg acataaaatc atgatcggcg tggtcgtatt tcccgatttc
     5221 cagttgctgg acgcagccgg tccgatttcg gtgttcgaaa tcgccgcccg cttcgccggc
     5281 cagccgccat cgatcaaggt gctggcggta acgtcgggtc cggttcgcag ttcgtcgggt
     5341 gttgaaatgc tcgcccgcgg tctcaggccg tctggcgcga tcacaacctt gatcgtggct
     5401 ggaggcgagg gggtgcggac agcggcgacc tgcgaaaaga cgcttggctt cgtgcgaacc
     5461 atggtcaagc gtggcatccg cgtcgccagc gtctgctccg gcgcctacat ccttgccgaa
     5521 gcaggcgtgc tcgatggccg ccgcgccacc acgcactggc agcgcacccg gcattttgtc
     5581 agcgcctatc ccaagataaa gctggaggcc gaccggattt tcgtccgcga cggcaatatc
     5641 tggagttcgg ccgggatcag cgccggcatc gatctggcgc tggcgctggt ggcggaggat
     5701 tttggcgaag agatcgcgca aaaaaccgcg cgtcaactcg tgctctatca ccgccgcggc
     5761 ggcggccagt cgcaattctc ctcgctcttg gaattgaagg cgcccgcggg gcgttttggt
     5821 ccgctattgg cctgggcgcg cgagcatctc gatgcgccgc tgacggtgga agacatggct
     5881 gaccaagccg gcatgagctc gcggcatttt gcccgggcct tcattgccga gaccggcacc
     5941 acgccgtcga aggcggtgga gcggctgcgg atcgaggtgg ccaggcagcg cgtgcaatcc
     6001 tccagcgagg cgatcgagcg cgtcgcgcaa gtaaccggct tccgcgatcc ggagcggatg
     6061 cgccgcgcct tcatccgcgc cttcggccag ccgccgcaat cactgcgccg cgcggcacgg
     6121 gcgggttagg gccaacgtgg cgcgaggtcc gaaaagtgcc gacaggcgac atcgacacac
     6181 ggggtaagac ttgatcatgc cattcagagt gtaacttggg gcttagaagg cacgggaggg
     6241 cttttgtgaa gaaacaagga gtgaagcacc atgtcgacta acgcgacgac tttggcgcgc
     6301 acgatcatgg caatgcggcc aatggtacct gccaaggatt ttgaaatcag caagcgattc
     6361 tatatcgaac ttgggtttga gcccaagatc ctaaccgacc gactcgtcga gatgcaactc
     6421 ggcgcattct ccttcattct gcaggcgtac tatgtccggc agtgggccga taacttcgtg
     6481 atgcacatgc gcgtctccga tgtgaagctc tggtgggatc acatcgttgg cctcgacctc
     6541 gcctctcgct acggtgtcaa aacgcgagca ccacaaatgg aggattgggg tttggtggcc
     6601 gccgtgatcg atccctccgg ggtgctttgg cggatcgccg aggcgtccgc tcctcatccg
     6661 aactgagaca cgctgcaacg gtgaaccggc cgactagctg gcgcgttgga tatcgaggat
     6721 atccaatacg gggcaatcgg gcgccgtctc gccggaacag cgcgctagtg tcttcgatag
     6781 cacgcgttcc agtttcttca ggtcgctcag tttggcccga atgtcttcga ggtggcgcgc
     6841 tgcgatctcg cgtacctcgc gacacgaagc cttgccaggc ccgcccaggc gcaaaagggc
     6901 gcgaatttca ccaagcgaga aacccagctc gcgcgaacgt cgaatgaaaa ccagagtacg
     6961 aaggtccgtc gtgtcataga cgcgacggcc gccagccgtg cgcggcggtg gggccagcat
     7021 ctcgacccgc tcgtagtaac ggatg
//
LOCUS       DASWGM010000005         9964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_555088,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000005 DASWGM010000000
VERSION     DASWGM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555088"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1729
                     /locus_tag="VGA15_00115"
     CDS             203..1729
                     /locus_tag="VGA15_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586135.1"
                     /translation="MADPKFPYIKWRDGHPRSSHGAYARALGFVDADLRHPPHDENGR
                     AVGAWFNLQEASDFSDKRKSEIEAARRAGKLPKKITVRKRTTIEDLLDDWSKSAEFKA
                     LSAASQSSYRKCISAILYRPQTREAAAKMRAEIRAAKLLGVAEPARELEAIATATPSS
                     IGKPELRAFYNYAKSIRGHHMALAMIATLSAAFTWGQESILWRLGPNPRAGMEFDHPD
                     GRIVQVAMPEFSAWVAAADAIERASIGDSFYLALFTGQRQTDRLIMRDESDVAARHAF
                     RQSKTGELVDIKEAPQLSARLNASRARIKALKLRLQLETVPLEFLVNEDNGKPYDEST
                     YRHWVSTARAVAVFGFLARDTVRQAIVRAERLTTELIRTIDRLFAPFPLDEENKAQRA
                     RAVDQRARMLAEWLDAQAARDSNGDGAAWRLKPCSSLMFVNGAGELDQKHDQDLRDTC
                     VMLLDRAGCDLLTICDITGHSYRSAQTIVKHYRARNADRADAGIDRLELQVRKEGMKG
                     "
     gene            complement(2038..2113)
                     /locus_tag="VGA15_00120"
     tRNA            complement(2038..2113)
                     /locus_tag="VGA15_00120"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2078..2080),aa:Lys,seq:ctt)
     gene            complement(2296..2880)
                     /locus_tag="VGA15_00125"
     CDS             complement(2296..2880)
                     /locus_tag="VGA15_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9586136.1"
                     /translation="MRRFAIAGIAALAIAASTAVYAQHHRWFHEHMRMSPEDRAAFTD
                     ARIAAVKAGLKLNPDQEKLWPPVETAVRDFAKLRIDRANARMNAKPDDSQDAQKQDSQ
                     NLPDPVARLRDRADAMAASAAAMKRIADAADPLYKTLDDSQKRRLAILTHMDGRFGGG
                     DWRHHGFMRGMDRDDGDRDRGTDRDGGRGGPERL"
     gene            complement(3121..4734)
                     /locus_tag="VGA15_00130"
     CDS             complement(3121..4734)
                     /locus_tag="VGA15_00130"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9586137.1"
                     /translation="MSPFKGILLAACVLASLLAVGPTRSAHAQRAATETAAVDQSTQS
                     PPPAQRSLQTAARPANPGNPPGVIGPEQRVALVIGNSNYQNVSQLSNPANDAKAIAKL
                     LNAAGFEVISATDLSHNEMVQVVQDFSSKIADRGPNTVAMVYYAGHGVQLAGENYLVP
                     VDARISSETDLVNGSVRLVDVMATLDAIPSRLRIAILDACRNNPFPSLNEAGRGLAIV
                     DAPNGSIVAYSTAPGSEALDGVGDHSPYTAAFLRLAHEKNLPIEQLFKRIRLDVNSST
                     GGQQTPWESSSLTSDFYFFGDTTLAATRAPAQEKAVYTADDLPSRASRAAYDYVLAEN
                     SIEHYQEFIQLYPHDPLCYRIRALLDSLMQAKAWHDAVLTNSPVAYKSFYDKFSSSPY
                     AQSALKLAEQPKMVSLSQPTHIMVPPSLKLGGLASGNIHAAPGKIVNLPVGGLNKSDA
                     LQNSKLDREVSRIDHRPAKSFAQIRDHQGPTRMSRPSFGRPPRSGNLRFASTQLHGSA
                     FRPQFRPQFRSGGGGFGGGGFGGGGFGRR"
     gene            complement(4830..6113)
                     /locus_tag="VGA15_00135"
     CDS             complement(4830..6113)
                     /locus_tag="VGA15_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9586138.1"
                     /translation="MSAALIFGLLFALMLTGMPISISLGLTVLTFLFTMTEVPIESVG
                     LKLFSGLDNFGIMAIPFFILAGTFLTRGGVARRMIAFTTSLVGHLPGGLGLAGVAACA
                     MFAAISGSSVATVVAIGSIMMPAMVEHGYPRRFGVGVISTSGALGILVPPSIILVLYG
                     VSTNTSIGALFMAGIVPGIMLASMLAVVTWFIAWRNGYPRMQRATFREQWSAFRESVW
                     GLLLIVIVLGGIYGGIFTPTEAAAVAAVYAFFITVFVYKELKLREVSKVLLQAASMSS
                     MLLYIITNAVLFSFLMTHEQIPQEMAAWIIDKNFSLWMFLLVVNILLLMAGNVMDPSS
                     ILLIMAPILFPLATKIGIHPVHLGVLMIVNTEVGLCHPPVGLNLYIASGIAKMGISEI
                     TIAVLPWLCAMLLFLGLITYIPEISLWLPRLLGMI"
     gene            complement(6113..6682)
                     /locus_tag="VGA15_00140"
     CDS             complement(6113..6682)
                     /locus_tag="VGA15_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9586139.1"
                     /translation="MLRILDRLEEIIITSLMAAATILIFVSVVHRFGTGVPFLYPYLI
                     AIHISWAQELCIYMFIWMAKFGAAYGVRTGIHVGVDVLINQFNPRWQKRVILFGLCGG
                     ALFTFIVGSMGAKFVFELMSTDQVSPDLELPSWLVYACIPLGSYLMCFRFLQVSWTYF
                     RTGDLPHHDATHVDGIEVSSKAPVALGEA"
     gene            complement(6829..7830)
                     /locus_tag="VGA15_00145"
     CDS             complement(6829..7830)
                     /locus_tag="VGA15_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9586140.1"
                     /translation="MHKLILAAASVAALALAGPASAQSPIVIKFSHVVASNTPKGAAA
                     EKFKELAEKYTDGKVKVEIYPNSTLYKDKEELEALQLGAVQMLAPSNSKFGPIGVREF
                     EVFDLPYILPDLATLRKVTDGPLGAKLLKLLEPKGMTGLAYWDNGFKQMTANKKLVTP
                     DDYKGLKFRIQSSKVLEAQFRSLGSIPQVMAFGEVYQALQTGVVDGQENTWSNIYTQK
                     MHEVQKYATITNHGYIGYVVVTNKKFWEGLPADIRTQLEKAMKDATAFGNGQSAKENE
                     DALEQIKKAGKTEIVSLTPAQDAAMRKAMDPVYKDVAGRVGQPLIDEFIRETGGVSH"
     gene            complement(8240..9265)
                     /locus_tag="VGA15_00150"
     CDS             complement(8240..9265)
                     /locus_tag="VGA15_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9586141.1"
                     /translation="MSLQTIDFGATHSRRGGSTGESARLRRIDLTTLRLFTAVCEEKN
                     LTRAAAREGIAASAVSKRMADFELAFGVTLFERLSKGMTLTPAGEALLHHARVALLNI
                     EKIAVELTEYSQGVRGHVRMLANLSAIVQFLPEDLSGFFNAHEFLKVDLQERPSGQVV
                     RGIEEGAAEIGICSGEAETRSLETFHYRYDNLVLVVRPDHPLAARDRLLFADTLDFDH
                     IGLHAASSIYLMSQYAATQAGKSMRLRINVPGFDAVCRMAQANMGIGLIPDRAFDVIG
                     AGMNLRSIRLRDEWARRKLKIVVRDARHLSTTGRLVLDHLRAAERSEQPAERKQRARD
                     RGAAPRC"
     gene            complement(9491..>9964)
                     /locus_tag="VGA15_00155"
     CDS             complement(9491..>9964)
                     /locus_tag="VGA15_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX9586142.1"
                     /translation="AFVRMKALADRKKDIYDEDIEALVDQEIAASHDRIKLTSLTVIA
                     GTHGPQRATMKLDVDGQIKIEEAEGNGPVDAVFNCIKLLVPHEAKLELYQVHAVTEGT
                     DAQAEVSVRLAHEGRSMTSKASDPDTLVASAKAYLGALNKIVMKRQRDIPAAAAS"
BASE COUNT         2023 a         3082 c         2955 g         1904 t
ORIGIN      
        1 ggcttcgacg cctggctgac tcggcatcat cccatgcgcc cgaagacgat cgctaatgac
       61 acattcgcgc caccgccggc agcaagcgat gaagaacatc gcgagcggct gagaacggcc
      121 tacgggcacg atggcgcttg acattcgaga tcgtgctaga ttccaatggg tgccttctct
      181 tacttcggca ccgagggttt ctatggccga tccgaaattt ccctatatca agtggcgtga
      241 cggccatccg cgctcatcgc atggcgctta cgccagggca ctcggctttg tcgatgccga
      301 cctgcgccat ccgccccacg atgagaatgg ccgcgcggtc ggcgcgtggt tcaatcttca
      361 ggaggcctcc gatttttccg acaaacgaaa atcggaaatc gaagcggcgc gccgcgctgg
      421 caaactgcca aaaaagatta cggtccggaa gcgtaccacg atcgaggatc ttctggacga
      481 ctggagcaaa tcggccgagt tcaaggcgtt gtcggccgcc tcgcaatcgt cctatcgcaa
      541 gtgcatctcc gccatcctct accggccgca gacgcgtgag gccgctgcaa agatgcgtgc
      601 cgagatccgc gccgccaagc tgctgggcgt ggccgagccc gcgcgcgaac tcgaggcgat
      661 cgcgaccgcc acgccatcgt caatcggcaa gccggaactc cgagcgttct ataactatgc
      721 gaaatcaata cgcggccatc atatggcgct ggcgatgatc gcgacgctgt cagccgcatt
      781 tacttggggc caggaaagca tcctgtggcg cctcggaccc aacccgcgag cgggcatgga
      841 gttcgatcat ccggacggcc gcatcgtgca ggtcgcgatg ccggaatttt ccgcgtgggt
      901 cgccgcagcc gatgcgatcg aacgggcctc gatcggcgac agcttttatc tcgcgctgtt
      961 caccgggcag cgccagaccg accgtctgat catgcgcgac gagagcgatg tcgcggcgcg
     1021 gcacgcgttc aggcaaagca agactggcga actcgtcgac atcaaggagg caccacaact
     1081 cagcgcacgg ctaaacgcct cgcgcgcgcg aataaaggcg ctcaagctga ggcttcagct
     1141 tgagacggtg ccgctcgaat tcctcgtcaa cgaggacaat ggcaagcctt atgacgagag
     1201 cacctatcgc cattgggtgt cgaccgcgcg cgccgtcgcc gtgtttggct ttctcgcacg
     1261 cgataccgtg cgtcaggcga tcgtccgcgc ggagcgactg accaccgaac tgatcagaac
     1321 catcgacagg ctgttcgcgc ctttccctct cgatgaagag aacaaggcgc agcgcgcccg
     1381 cgcggtcgat cagcgcgcgc gtatgctggc ggaatggctg gatgcgcagg cggcgcgcga
     1441 cagcaatggc gatggggcag catggcgcct gaagccgtgc tcgtcgctga tgttcgtcaa
     1501 tggcgccggc gaactcgacc agaagcacga ccaggacctg cgcgatacct gcgtcatgct
     1561 gctcgatcgc gcgggctgcg acctgctgac gatctgcgac atcacggggc actcgtaccg
     1621 aagcgcacag accatcgtga agcattaccg cgcccgcaat gccgatcgcg ccgatgccgg
     1681 catcgaccgg ctcgaattgc aggtgcgcaa ggaagggatg aagggctgag cgccgacggg
     1741 cccccgggtt cggcgcagtg ggtgtaattc cgcagccgca gcatcagctc tagcatcttt
     1801 cctggtcaag gagcctcggc taggcgcagc gctccgctta cttctggcga tgatattcgc
     1861 tgcgaacctt cttgctcatc gaaggctgct gcgcaccggg ttccatgaag gacatttaga
     1921 gaacattatg agctcgtcgc acggcgtgcg acgtgcgacc acgaaaagcg tcgcacgaga
     1981 cccgttttgt tctggaccga catcccgcga aggtactgga aaagaaggcc ttttccttgg
     2041 tgggcgcacc agggctcgaa cctgggaccc gatgattaag agtcatctgc tctaccaact
     2101 gagctatgcg cccggaaacc ggcccggaaa gccttcgcaa gagggcgtcg tttagcaaag
     2161 cgaccccccg atgtccagca aggtaagtcg atttccccgg cataacccgc tgaaagggaa
     2221 aacgaaaaag ccgccggaat ccgaacggct ttttgctcat ttcaatcgat cgggggcgat
     2281 cgagggttga cgcggctaaa gccgctcggg gccacctcgg ccgccatccc gatcggtgcc
     2341 gcgatcccga tcgccgtcat cacggtccat accgcgcatg aatccatggt gccgccaatc
     2401 tccgccgccg aaccggccat ccatatgcgt cagaatcgcg aggcggcgct tctggctgtc
     2461 gtcgagggtc ttgtagagcg gatcagccgc atctgcgatc cgtttcatcg cggccgccga
     2521 ggccgccatg gcatcggcgc ggtcacgcag gcgggctacc ggatcgggca aattctgaga
     2581 atcttgcttc tgcgcatcct gggaatcatc cggcttggca ttcatccgcg cattggcgcg
     2641 atcgatccgc agctttgcga aatcccgcac cgcggtctcg accggcggcc acagcttttc
     2701 ctggtcgggg ttaagtttca gccctgcctt gacggctgcg atccgcgcgt cggtaaaggc
     2761 cgccctgtcc tccgggctca tccgcatatg ttcatgaaac caccgatggt gctgggcata
     2821 gaccgcggtc gaagcagcga tggcgagtgc cgcgatgccg gcgatagcaa acctcctcat
     2881 ccgaacctcc tttgaaaagg ttgaccgaag atgggcgctc gcgcgcccgc aatcaactta
     2941 cagtttggac agatgagctt gtttaaccaa gatgtaatct gccgcgacgt tctttcgacc
     3001 ggagagaaaa gtcaaaacca cccgcccaag accgcgtctg ccttcggcgg gtggcgggtt
     3061 tgcaagcaac cttgtttgta accttgtctt gtcaggaatc gttggcgggc ggcaggacac
     3121 tcaccgtcgt ccaaacccgc cgccaccgaa cccaccgccg ccaaatccgc cgccgccgga
     3181 ccgaaactga ggtcgaaact gaggtcgaaa cgcggatcca tgaagctggg tcgacgcgaa
     3241 gcggaggttg ccacttctcg gcggcctgcc gaagctgggc cgcgacatcc gcgtcggccc
     3301 ctgatgatcc cgaatttgcg cgaacgattt cgcaggccga tggtcgatgc ggcttacctc
     3361 tcgatcaagc ttcgaattct gcaacgcgtc ggatttgttc aaaccaccga ccggcagatt
     3421 gacgatcttg ccgggagcgg cgtgaatgtt tccggatgcg agaccgccga gcttgagcga
     3481 aggtggcacc atgatatgcg tgggttgcga gagcgaaacc attttcggct gctccgccaa
     3541 cttcaaggca ctctgtgcat agggactgct cgagaacttg tcgtagaacg atttgtacgc
     3601 gacgggagaa tttgtgagca ccgcatcatg ccaggccttg gcctgcatga ggctgtccaa
     3661 cagcgcgcgg atgcggtagc agagcggatc gtgagggtaa agctggatga attcctgata
     3721 atgctcgatg gaattttcag ccaatacgta gtcataggcc gcgcgcgagg cacgcgacgg
     3781 gagatcatcc gccgtgtaga cggctttctc ctgtgccggc gctctggttg ctgccagcgt
     3841 ggtatcgcca aagaaataga aatcgcttgt cagcgacgag ctttcccagg gggtctgctg
     3901 cccgccggtt gaactgttaa cgtcaagacg aatgcgcttg aacaattgct cgatcggcag
     3961 gtttttctca tgggccagcc gcaagaacgc agcggtgtaa ggactatggt cgccgacgcc
     4021 gtcgagcgct tcgctacccg gcgcagttga ataggctacg atcgagccgt tcggcgcatc
     4081 gacgatcgcc agtccgcgtc cggcctcgtt gagcgaggga aacggattat tgcggcaggc
     4141 atcgaggata gcgatccgca ggcggctcgg gatcgcgtcc agcgtcgcca tcacgtcgac
     4201 taaccggacc gaaccattga ccagatcagt ctcggaggaa attctggcgt cgacgggaac
     4261 gagataattc tcgcccgcga gttgcacgcc gtggccggca taatacacca ttgccacggt
     4321 gttcggcccg cgatcggcaa tcttgctgga gaaatcctgc acgacctgaa ccatctcatt
     4381 gtgactgaga tccgtcgctg aaatcacctc aaagcccgcg gcattgagga gttttgctat
     4441 cgccttcgcg tcattggccg gatttgagag ctgggacacg ttttggtagt tcgaattgcc
     4501 gatgaccagc gcgacgcgct gctccgggcc aattaccccc gggggattgc ctgggttggc
     4561 cggcctcgcc gctgtctgca gtgatctctg ggccggcggc ggcgattgcg tcgactgatc
     4621 cacggcggcg gtttcagttg ctgcccgctg ggcatgagcc gaccgcgtcg gcccgaccgc
     4681 cagcaggctg gccaaaacac aggcagccaa taaaattccc ttgaatgggc tcatagcatt
     4741 ctcccacaac aaatctcggc gtgagattgg ttgcgagcac gctagtcggg gttcatcctg
     4801 cggtatgtga tcttggtcac gacggggcct cagatcatgc cgagcaggcg cggcagccac
     4861 agcgatattt cgggaatgta ggtaatcagc cccaggaaca acagcatcgc gcacagccag
     4921 ggcagcaccg cgatggtgat ttcactgatg cccatttttg cgataccgct ggcaatatag
     4981 agattaaggc cgaccggcgg atggcacagt ccaacctcgg tattcacgat catcagaacg
     5041 ccgaggtgaa ccggatggat gccgatcttg gtcgcgagcg gaaacagaat cggcgccatg
     5101 atcagcagga tcgacgacgg gtccatgacg ttgcccgcca tcaagagcag gatgttgacc
     5161 accagcagga acatccacag actgaaattc ttgtcgatga tccaggccgc catctcctgc
     5221 gggatctgct catgggtcat caggaacgag aacagcaccg cattggtgat gatgtagagc
     5281 agcatcgagc tcatactcgc cgcctgcagc agcacttttg agacttcgcg gagtttcaac
     5341 tccttgtaga cgaacaccgt gatgaagaag gcgtagaccg cggccaccgc ggcggcctcc
     5401 gtcggtgtaa agattccgcc atagatgccg ccgagcacga tgacgatcag cagcaggccc
     5461 cagacgcttt cacggaacgc gctccattgt tccctgaacg tagccctttg catccgcggg
     5521 tagccgttgc gccaggcgat gaaccaggtc accaccgcaa gcatcgacgc cagcatgatc
     5581 cccggcacga tgcccgccat gaacagcgcc ccgatcgagg tgttggtgga aacgccatac
     5641 agcacgagga tgatcgacgg cggcaccagg atgccgagcg cgccggaggt cgagatcacg
     5701 ccgacgccga accggcgcgg atagccatgc tcgaccatgg cgggcatcat gatcgagccg
     5761 atcgccacca cggtggcaac gctggatccc gaaatcgccg cgaacatcgc gcaggccgct
     5821 acaccggcaa ggccgagccc gcccggcaaa tggccgacca gcgaggtggt aaaggcaatc
     5881 atgcggcgcg cgacgccacc ccgggtcagg aaggtgccgg cgagaatgaa aaacggtatc
     5941 gccatgattc cgaaattgtc gaggccggaa aacaatttca gtccgacgct ctcgatcggc
     6001 acttccgtca tcgtgaacag gaaggtcaga accgtcaggc cgagcgaaat cgagatcggc
     6061 atccccgtca gcatcagggc gaacagcagt ccgaaaataa gagcagcgct catcacgctt
     6121 ctcccagcgc caccggcgcc ttcgagctga cttcgatgcc gtcgacatgc gtcgcatcat
     6181 gatgcggcag gtcgccggtc cggaaatagg tccacgatac ctgcagaaac cggaagcaca
     6241 tcaaatagga gccgagcgga atgcaggcgt agaccagcca gctcggcaat tccagatcgg
     6301 gcgagacctg gtcggtgctc atcaattcga aaacgaattt cgcacccatc gagccgacga
     6361 tgaaggtgaa cagcgcaccg ccgcacagac cgaacagaat gacgcgcttc tgccagcgcg
     6421 gattgaactg gttgatcagg acgtcgacgc cgacatggat accggtacgc accccatagg
     6481 ccgcgccgaa cttcgccatc cagatgaaca tatagatgca cagctcctgc gcccaggaga
     6541 tgtgaatggc gatcagatag ggatacagaa atggcacgcc ggtgccgaac cggtgcacga
     6601 cggaaacgaa aatcagtatc gtcgcggccg ccatcagcga ggtgattatt atttcctcaa
     6661 gccgatcaag gatacgaagc atctctcccc ctcatacgcc cagtgaactt tcgggcgcgt
     6721 cgccagtgac ttgctgccga accggcgccg gtcggtcccc gcggtccggc gccgttcaaa
     6781 tgaaaaagcg agctctcgca gctttcgcga gagctcgccg gcattgtgtt agtggctgac
     6841 cccgccggtt tccctgatga actcatcgat caagggttgg ccaacccggc ccgcaacgtc
     6901 cttgtaaacc gggtccatcg ccttgcgcat ggcggcatct tgcgccggcg tcaagctgac
     6961 gatttcggtc ttgccggcct tcttgatctg ttcgagcgcg tcctcgtttt ccttcgccga
     7021 ctgcccgttg ccgaaagcgg tagcgtcctt catcgccttc tcgagctggg tacgaatatc
     7081 agcgggcaga ccctcccaga acttcttgtt cgtgaccacc acatagccga tgtagccgtg
     7141 attggtgatg gtggcgtact tctgcacctc gtgcattttc tgggtgtaga tattcgacca
     7201 ggtgttttcc tggccatcga ccacgccggt ctgcagcgcc tggtacacct cgccgaacgc
     7261 catcacctgc gggatcgaac ccagcgagcg gaactgcgct tcaagaacct tcgacgactg
     7321 gatgcggaat ttcaggccct tgtagtcgtc cggcgtgacc agctttttgt tcgcggtcat
     7381 ctgcttgaag ccgttgtccc aataagcgag tccggtcatt cccttcggct cgagcagctt
     7441 cagcagtttc gcgccgagcg gcccgtcggt gactttgcgc aaggtggcga gatccggcag
     7501 aatataggga agatcgaaca cctcgaattc acgcacgccg atcggaccga acttggaatt
     7561 cgatggcgcc agcatctgca ctgcgccgag ctgcagcgct tccagttctt ccttgtcctt
     7621 gtagagtgtc gaattcggat agatttcgac cttgaccttg ccgtcggtgt atttctctgc
     7681 gagttccttg aacttctcgg cggcggcgcc tttcggcgtg ttcgaagcca ccacgtggct
     7741 gaacttgatg acgatcggcg attgcgccga cgccggtcct gccagggcca atgctgcgac
     7801 cgacgcagca gccagaatta atttatgcat gtttcctccc gcgtgacttt gcgaagcgcg
     7861 ccaacggcgc ggccgcgacg ccccattcca agcgcaccaa tacagagaag ccgaccggaa
     7921 cgccattgcc cctttagcag gggctgtttt gtgagttctg ttgcactgca atacgcgaca
     7981 taatgtcgtt ggtgactgct tgcgccattc gcggatcgac catcgcatcc atcccggcga
     8041 ttcagcgtcg aactcggcca gcctcctcga cgcaaacggc gccgcgatca agtctgctaa
     8101 gttcaataac gtgaatgcct gcaagcgggc cactcaactc cagcgttccc ttggattttc
     8161 tcatccgtat ctgccggagc ttttgccaaa tagtccaatt gtcgttaact aacgcgacgt
     8221 tcgtcgtcgg agaacgccgc tagcagcgcg gcgccgcgcc gcgatcgcgc gccctctgct
     8281 tcctttccgc gggttgctcg ctccgctccg cggcccggag atggtcgagc accagccggc
     8341 ccgtagtgga caggtgccgg gcgtcgcgca cgacgatctt gagtttccgc cgcgcccatt
     8401 cgtcgcgcag ccggatcgac cgaaggttca ttcccgcacc gatgacgtcg aatgcgcgat
     8461 ccggaatgag gccgatcccc atgttcgcct gtgccatccg gcacaccgcg tcgaaaccgg
     8521 gcacgttgat gcgcagccgc atcgacttgc cggcctgggt ggctgcgtat tgcgacatca
     8581 ggtagatcga actggcggca tgaaggccga tgtgatcgaa atccagggtg tccgcgaaca
     8641 gcagtctgtc gcgcgccgcg agcggatgat ccggccgcac caccagcacc agattgtcat
     8701 aacggtaatg aaatgtttcg aggctgcggg tctcggcctc gcctgagcaa atgccgatct
     8761 cggctgcccc ctcctcgata cccctgacca cctgcccgct cggccgttcc tgcaagtcga
     8821 ccttcaagaa ttcatgggca ttgaaaaaac ccgacagatc ctcgggcagg aactgcacga
     8881 tcgccgacag gttcgcgagc atgcggacgt gcccgcggac accttgcgag tactcggtca
     8941 attcgacggc gatcttttcg atattcagaa gcgcgacgcg ggcgtggtgc agcagcgctt
     9001 ctcccgccgg cgtcagggtc atgcccttcg agagccgctc gaacagggtt acgccaaatg
     9061 cgagttcgaa atcggccatc cgcttgctga cggcggatgc cgcgatgccc tcgcgcgccg
     9121 ccgcccgcgt caggtttttt tcttcgcaaa ccgccgtgaa cagccgcaac gtcgtcagat
     9181 caatccggcg aagccgcgcg ctttccccgg tgctgccgcc gcgccgagaa tgcgttgcgc
     9241 cgaaatcgat ggtttggaga gacattgaaa ggccctgtcg ctttgcaggt gacgccaatt
     9301 gatcggtcga cgttacattc acaacagatg caaaaaatct caaactggcg cgtcaatcat
     9361 gggcgtattg ggaaatgatt ccaaccgatg tctcactcag cgcgtttcat tccaattggg
     9421 tgcgaggacg agccgaaacg aaaaacgcgg cgccgaagca ccgcgtttgg cttggctgca
     9481 tctgaagcca tcagctcgct gccgccgccg gaatatcgcg ctggcgcttc atgacgatct
     9541 tgttgagcgc cccgagatag gctttggccg aggccaccag cgtgtccgga tcggatgcct
     9601 ttgaggtcat cgagcggccc tcatgggcga gccgtaccga aacctcggcc tgcgcgtcgg
     9661 tgccttcggt gacggcgtgg acctgataga gctccagctt cgcctcgtgc ggcaccagca
     9721 gcttgatgca gttgaacacc gcatcgaccg gaccgttgcc ctcggcctcc tcgatcttga
     9781 tctggccgtc gacatcgagc ttcatggtgg cgcgctgcgg cccatgggtg cccgcgatca
     9841 ccgtcagcga ggtcagcttg atgcgatcat gggaagcggc gatttcctga tcgaccagcg
     9901 cctcgatgtc ctcgtcgtag atgtccttct tgcggtcggc gagcgccttc atccgcacga
     9961 acgc
//
LOCUS       DASWGM010000006         6227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1109439,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000006 DASWGM010000000
VERSION     DASWGM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109439"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..567)
                     /locus_tag="VGA15_00160"
     CDS             complement(340..567)
                     /locus_tag="VGA15_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586143.1"
                     /translation="MAETLSERERGFNEGLQAAIDYCGGWAAVIGNIDDNASAQRTAL
                     LYLVAAYQSDLRALQKRRDAEPKTWQEDHLD"
     gene            566..1003
                     /locus_tag="VGA15_00165"
     CDS             566..1003
                     /locus_tag="VGA15_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586144.1"
                     /translation="MVQTQTLISARHEIASHLPDASVRQQTIQRGSVEHDQVFTGSKA
                     SVLDVRAFRLSLTESRPYRASSYRRCGSARAVATSALIAFCGVRLWLYLATSLAKIIS
                     AANDEGLTADAFRNVVGQRIRDLKREQNPQLPLGMTSINGGAA"
     gene            complement(1103..2332)
                     /gene="hemA"
                     /locus_tag="VGA15_00170"
     CDS             complement(1103..2332)
                     /gene="hemA"
                     /locus_tag="VGA15_00170"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX9586145.1"
                     /translation="MDYSKFFSAALSRLHDERRYRVFADLERIAGRFPHAVWHSPKGP
                     RPVVIWCSNDYLGMGQHPKVVGAMVETATRVGTGAGGTRNIAGTHHPLVQLEQELADL
                     HGKEAALLFTSGYVSNQTGIATIAKLIPNCLILSDALNHNSMIEGIRQAGCERQIFRH
                     SDMAHLEELLRAAGPDRPKLIACESLYSMDGDIAPLAKICDLAERYGAMTYVDEVHAV
                     GMYGPRGGGIAERDGVMHRIDILEGTLAKAFGCLGGYIAASSDIIDAVRSYAPGFIFT
                     TALPPAICSAATAAIRHLKISNWERERHQDRAARVKAILNAAGLPVMSNETHIVPLFV
                     GDPEACKRACDILLEEHHIYIQPINYPTVAKGTERLRITPSPYHDDGLIDQLAEALVQ
                     VWERLDLPLAEKSLAAE"
     gene            complement(2566..3438)
                     /locus_tag="VGA15_00175"
     CDS             complement(2566..3438)
                     /locus_tag="VGA15_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9586146.1"
                     /translation="MDRQTELGIIRAAYAKQILAAARVVNDPRLEAALGATRREDFLG
                     PGPWPIMRWFRDYVTTPDADPVYLYTDDLVGILPERQLNNGQPSLHAHLVHQASPAAG
                     EHVVHIGTGTGYYTAILAYLAGSSGRVTGIEYEPELAARAKANFAGYPNIEIIEGDGT
                     LVSFDQADVIYVNAGCTRPAQNWLDRLADGGRLILPMTSDQGFGATSLDRMASAGAVF
                     RIERRGHDYFARWISAVAIFPCAGNRDEPSERALAEAFDKGGWQHVTRLYRDENIPEE
                     RCWVSGPGWCLATS"
     gene            3533..4030
                     /locus_tag="VGA15_00180"
     CDS             3533..4030
                     /locus_tag="VGA15_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9586147.1"
                     /translation="MKRRLYPAVLERGRKGAFGAWFPDFPGCVAAGKSQEETIEKAED
                     KLAQAVDVLFEQGRPLPVSTSIERIVLPKGCDLLAYFVVGVDPPDPSERVNVYLPKSL
                     IGRIDRRAAELGMSRSSFFGLAAASALVRLPGPFWPEVAEPRSKVHKTGGLRTKKSST
                     KKSPA"
     gene            complement(4043..4573)
                     /locus_tag="VGA15_00185"
     CDS             complement(4043..4573)
                     /locus_tag="VGA15_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HEX9586148.1"
                     /translation="MLTATRILTNTPLWVFALLAYLIWQGSQSLRPRTQPIWRMLIVP
                     LVFFLMGLSRLVTARDRGIEPLLGWLVAAVLFALLALSLRPKLLAVDRKSGTVTRPGS
                     ATPLARNITVFVLQYAVAVATAMKLEPHAAVAIIGHAVSGASAGYFSGRAVALVRRYR
                     NFDTGGNSGARIADGA"
     gene            5013..5291
                     /locus_tag="VGA15_00190"
     CDS             5013..5291
                     /locus_tag="VGA15_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586149.1"
                     /translation="MTGVTALAWRAVHAATIEIGRNGRLERGSQEEFSMAKFAIGEKV
                     DNAANDHEGGIVIAVFPTVEGNFRYAVDTEGYGTLNFFSEEKLVLHLN"
     gene            complement(5361..5603)
                     /locus_tag="VGA15_00195"
     CDS             complement(5361..5603)
                     /locus_tag="VGA15_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586150.1"
                     /translation="MAASNRAIIHSAGAGRQFGVENLPICETCLRSMQFTRRTPHPIF
                     GDGYELQLFECRTCGRQNRRSTDANGLPHHGGVLLT"
     gene            complement(5841..>6227)
                     /locus_tag="VGA15_00200"
     CDS             complement(5841..>6227)
                     /locus_tag="VGA15_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586151.1"
                     /translation="ASGRQDHTILPSAATSLVGVLLTAHGFKEPALRSHRAQNAAAST
                     ASHPAFVTIMIRPSVWDGMRKVLEVIWGVRKQEYFCIQDWTGSISLIRFNKFAVARKS
                     EQRGGAPSPAGEVSKDGWFKAFALGD"
BASE COUNT         1186 a         1872 c         1987 g         1182 t
ORIGIN      
        1 gccgaaaaac gtccaggacg tgcaatagat cgccagcgac agcggataga tcagcgcgct
       61 ggcgcgcccg cgctgcgtcg gcgagaggcg gtcgccatgg ctcgcgacga aaaacaggaa
      121 cccgatgtag ccgaaggcgg ctgcgatcac gccccagtca tgcagcatcg ctgcttacct
      181 cccgagatca cgatccgatg cgacggatca cgggtcaatg cgaaccaccg ccgaccaggg
      241 cagggagtat agccggatcg agccggcaaa gcatccgatg tcgccgcccc ggatgcggga
      301 ttcacccagg aatagaccat gccacgtggg atccggatgt taatctaggt ggtcctcctg
      361 ccaagttttt ggctcggcat ctctgcgttt ttgcaaggcc cgcagatcgg actgataggc
      421 ggccaccagg tacagcagcg cggtgcgctg ggcggaggca ttgtcgtcga tgttcccgat
      481 cacagctgcc cagcccccgc aatagtcgat tgcggcctgc aggccttcgt tgaaaccccg
      541 ttcgcgctcg ctcaaagttt cggccatggt tcaaactcaa actctgatct cggcgcggca
      601 cgaaatagcc tcacacctgc ccgacgccag cgttcgccaa caaacgatcc agcgaggaag
      661 cgtcgagcac gatcaggttt ttacaggtag caaggcgtct gtcttggacg tacgcgcctt
      721 tagattgtcg ctaaccgaat cgcggcctta ccgggcctca agctatcgcc ggtgtggatc
      781 agctcgggcg gttgcgactt cggccctgat cgccttctgc ggcgttcgct tgtggttgta
      841 tttggctaca tcgctggcca agatcatttc agccgccaac gacgaagggc tgacagccga
      901 tgctttccgc aacgtcgtcg ggcaacgcat ccgcgatctc aaacgcgagc agaaccctca
      961 actgccgctg ggcatgacgt ctatcaacgg cggtgcggct tgaggttctc cagatttgtc
     1021 aagagattac cccaatatgt tacaacccct atagatttct gtaacattta gggcgatagg
     1081 agttaacgct atggggttaa cgctattcgg ccgccagcga cttttccgca agcggcaggt
     1141 cgagacgctc ccagacctgc accagcgctt cggcgagctg atcgatcaat ccgtcgtcgt
     1201 gatagggcga aggcgtgatc cgcagccgct cggtgccttt ggcgacggtc ggatagttga
     1261 tcggctggat gtagatgtgg tgctcttcca gcaggatatc gcaggcccgc ttgcaggctt
     1321 cgggatcgcc gacgaacagc ggtacgatat gggtctcgtt cgacatcacc ggcagccccg
     1381 cggcgttgag gatcgccttg acccgggcgg cgcggtcctg atggcgttcg cgctcccagt
     1441 tcgagatttt gaggtgccgg atcgcggcgg tggccgccga acagatcgcg ggcggcagcg
     1501 cggtggtgaa gataaagccg ggcgcgtagg aacgcaccgc atcgataata tcggacgatg
     1561 cggcgatata gccgccgaga cagccgaacg ccttggccag cgtgccttcc agaatatcga
     1621 tccggtgcat gacgccgtcg cgctcggcga tgccgccgcc gcgcggacca tacatgccga
     1681 cggcgtgaac ctcgtcgaca taggtcatcg cgccgtagcg ctcggcgaga tcgcagattt
     1741 ttgccagcgg cgcgatgtcg ccgtccatcg aatacaggct ctcgcaggcg atcagtttcg
     1801 gccggtcggg gccggcggcg cgcaagagtt cttccagatg cgccatgtcg ctgtggcgga
     1861 agatctggcg ctcgcagccg gcctggcgga tgccctcgat catcgaattg tgattgagcg
     1921 cgtccgacag gatcaggcaa ttcggaatga gcttggcgat ggtcgcgatg ccggtctggt
     1981 tcgagacata gcccgaagtg aacagcagcg cggcctcctt gccatgcaga tcggccagtt
     2041 cctgctcgag ctgcaccaac ggatggtgcg tgcccgcgat gttgcgggtg ccgccggcgc
     2101 cggtgccgac ccgcgtggcg gtctcgacca tggcgccaac caccttcgga tgctgcccca
     2161 tgccgagata gtcgttggag caccagatca cgaccgggcg cggccccttc ggggaatgcc
     2221 acaccgcatg cggaaaccgg cccgcgatcc gctcgagatc ggcgaaaacc cggtagcgcc
     2281 gctcgtcatg caggcggctg agggcggcac tgaagaattt gctgtaatcc atcgcaagac
     2341 ctgaaacgga gcgtgaggcc gggagcggca aaaccttttt agagcgtttc caggtttgat
     2401 gtcgagccgg aattggcttt ttcggtcgcg ctatggttgg cagagggttg catcctgcgc
     2461 gcaccaaaat ttgatctgga tcaagacgtt ggatcgactt tgccgtccgc ggtattttgg
     2521 ggaaagtgag gcggttttgc agcgaacctc accaccgccc gaacgctagc tggtggcgag
     2581 gcaccatccg ggtccgctga cccagcagcg ctcttcggga atgttttcgt cgcgatagag
     2641 gcgggtgacg tgctgccagc ctcccttgtc aaaagcttcg gccagcgcgc gttcggatgg
     2701 ctcgtcgcgg tttccggcgc agggaaagat cgcaaccgcc gaaatccatc tcgcaaaata
     2761 gtcatgaccg cgccgctcga ttcgaaacac cgcgccggcg ctcgccatcc tatccagaga
     2821 cgttgcccca aaaccctggt ccgacgtcat cggcaggatc aaacgtccgc cgtcggcgag
     2881 acgatcgagc cagttttggg ccggccgcgt gcagccggca ttgacgtaga tgacatcggc
     2941 ttgatcgaac gacaccaaag tgccgtcgcc ttcgatgatt tcgatgtttg ggtagcctgc
     3001 gaaattggct ttggcgcgcg cggcgagttc aggctcatat tctattccgg tgacccggcc
     3061 tgacgatccc gcgaggtagg ccaggatggc ggtgtaataa ccggtgccgg ttccgatatg
     3121 cacgacgtgt tcgccggcgg ctggcgaagc ctggtgaacg aggtgcgcat gcaacgacgg
     3181 ttgcccgttg ttgagctgcc gttccggcag aatgccgacg agatcgtcgg tgtacaaata
     3241 aaccggatcg gcgtcgggcg tcgtgacgta atcccggaac cagcgcatga tcggccacgg
     3301 acccggcccg agaaaatcct cgcggcgtgt ggcgccgagc gcggcctcga gacgcggatc
     3361 attgacgacg cgcgcggcgg ccagaatctg tttggcgtag gccgcccgga tgatgccgag
     3421 ttcggtttgg cggtccatgg gactctttcg cccccttgtg ggttgattct gggctggtgt
     3481 gtgattaaac acacccatgt gtgtgaaata gcacaagctc acggagcggc caatgaaaag
     3541 acggctttat ccggcggtcc tggagcgcgg tcggaagggg gccttcggcg cctggtttcc
     3601 cgatttcccc ggctgcgtcg ccgccgggaa atcgcaggag gaaaccatcg aaaaggcgga
     3661 ggacaagctg gcgcaggcgg tggatgtgct gttcgaacag ggcaggccgt tgccggtgtc
     3721 gacctcgatc gagcggatcg tgctgcccaa gggctgcgac ctgcttgcct attttgtcgt
     3781 gggggtggac ccgccggatc cgtcggagcg cgtgaacgtg taccttccga agagcctgat
     3841 cggccggatc gaccggcgcg ccgcggaact cggcatgagc cgctccagct ttttcgggct
     3901 cgccgccgcc tcggcgttgg tccggctccc gggcccgttt tggccggagg tggcggagcc
     3961 ccgctcgaag gtgcacaaga cgggtgggct gcgaactaaa aaatcctcga ccaaaaaatc
     4021 gccggcgtaa cgggtcggcc tctcaagcgc catcggcaat gcgtgcgcca gaatttccgc
     4081 ccgtgtcgaa attcctgtaa cggcgcacca gcgccaccgc ccggccggaa aagtaacccg
     4141 cgctggcgcc ggagacggcg tggccgatga tcgccacggc ggcgtggggt tcgagcttca
     4201 tggccgtcgc caccgcgacg gcgtattgca gcacgaatac cgtgatgttg cgagccagcg
     4261 gggtggcgct gcccggacgg gtcacggtgc cgctttttct gtccacggcg agcagttttg
     4321 gcctaaggga aagcgccagc aaggcaaaca agactgccgc caccaaccac ccgagaagcg
     4381 gctcgatccc cctgtcgcgc gccgtgacca gccgcgacaa gcccatcaga aaaaacacca
     4441 agggcacgat gagcatccgc cagatcggct gcgttctcgg ccgcagcgat tgcgagccct
     4501 gccagatcag gtaggccagc aacgcaaaaa cccagagcgg ggtattggtg aggatgcggg
     4561 tggccgtcag catcggctct cctgatggcg tgttgtccgg cccgttgtga cgcgaaacgc
     4621 gggtatgtta tatcgcacgc gctcatgcgg gctacttgtt ggcatcgagg tagggcgagt
     4681 cgtatcaaaa cgctagcgtc attccccgat gtgcaattgc acatcgtgga tgcgcctctt
     4741 ggcgcaggcc cggaatccat actccctgtc gtggttatgg attccgggct cgcgcggagc
     4801 ctgtcatcgg gcggcgcaga gcgccgaccc gttggcgcgc cccggaatga cggattgaag
     4861 caagcgcagc ccggatgagc taacgggtcg cgcgaatgcg cgccccgttc atgagctccg
     4921 cgacatccgg gattttcata tttcctttga gccccgcata tcgctccgca gcgagcgggg
     4981 accgtgcagg ccgccaagac caggtacctg acgtgaccgg cgtcacagcc ctcgcgtggc
     5041 gggcagttca tgctgccacc atcgagattg gccgcaacgg ccgtcttgaa agaggcagcc
     5101 aggaggagtt ctcgatggcg aaatttgcga ttggcgagaa ggtcgacaat gcagccaacg
     5161 accatgaggg cggcatagtg attgccgtgt ttccaaccgt tgaaggcaat ttcagatacg
     5221 ccgtcgacac cgaaggctac ggcacgctca atttcttcag cgaagaaaaa ctggttcttc
     5281 acctgaattg agccgcgcgt cgatgcggtc gtggcttcgc ggaagtgctc ggcggcccgt
     5341 ccctgcccgc gcagcgaccg tcacgtcagc agaacgccgc catgatgcgg gagaccgttg
     5401 gcgtccgtgc ttcggcggtt ctgtcggccg catgtccggc attcgaacaa ctgcagctca
     5461 tagccgtcgc cgaagatcgg atgcggcgtg cgccgggtaa attgcatgga tcgtaggcac
     5521 gtttcgcaaa tcggcaggtt ttccacgccg aactgccggc cggcacctgc cgaatggatg
     5581 atcgcacgat tgctggcagc catgatgatg tcctctggtc ggtggcaatc aacgcgctac
     5641 cctgaagagt gttccgagtt gaaaacagct ggtatgcgga tcgggttccg gaaaattggc
     5701 cgattggagc gcgcgcgggc ggatgccaga gatgccggcc gagtaacgtc attgttcgtc
     5761 gtgccagacg ctgacgatcc cagccagtcc ttcgctttgc tacgaagccc atgccttcga
     5821 atgcatccat tgcggttgct ttaatccccg agcgcgaagg ctttgaacca gccgtctttg
     5881 ctgacctcac cggccggcga cggggctcca ccccgctgtt cggacttgcg cgcaacggcg
     5941 aatttattga accggatcaa gctgatgcta ccagtccagt cctgaatgca aaaatattcc
     6001 tgttttcgca ccccccaaat cacctctaga acttttcgca tcccgtccca cacagagggg
     6061 cgtatcatga tcgtcacgaa cgcgggatgg gatgcggtgg acgcggcagc gttttgcgcg
     6121 cgatgggatc gcagggcggg ttctttgaat ccgtgagcgg tcaacagcac gccgacgaga
     6181 gatgttgctg cggacggcaa aatcgtgtgg tcctgacgcc ccgacgc
//
LOCUS       DASWGM010000007         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1663964,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000007 DASWGM010000000
VERSION     DASWGM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663964"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VGA15_00205"
     CDS             <1..123
                     /locus_tag="VGA15_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9586152.1"
                     /translation="VHMDGNLCRCMTYSRIQKAIMRAATEMRTASVGGNERRPT"
     gene            120..2402
                     /locus_tag="VGA15_00210"
     CDS             120..2402
                     /locus_tag="VGA15_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9586153.1"
                     /translation="MNRHVKVTPQTTDLSRRSFLVGTAAAGLVLGFAGVSGIDQAFAA
                     ASTNFEPSVWYAISQDGLVTVTCGKADMGQHIASTMAQLICEELGAKWTDMRVQLASN
                     DPKFNDPVLGAQITGASWSTMMNFDAMSRAGAAGRMALTEAAAAAMSVPAAELVVRDS
                     TISHTKSKKQMSFADVVKSGKATKTFTPDELKAIKLKTLDQYTMIGVSVPQLDIPSKT
                     DGTAKYGIDVMLPGMVHGAIVTPPVHFGATVKLVDDSSAKKVPGFIKAVTLDDKTGST
                     TGWVVAVANTYAQAKKAAEALKISYDGGANAKLSSQSLLDEAKRLQGLSDAGQFFVKD
                     GDPAAALGTASKVLEAEYTTNINIHCPMEPMNATAEFKGDILHIYSGNQFATRSGAIA
                     AGAAGIDPKFVVMHQMWLGGGFGRRLDADMMVPAVQAAKAVGKPVKVIYSRENDMTMD
                     YSRPLTYQKVKAGVDGDGKLIAISHDVVSAWPTARWGIPDFLTPSVDKKGPLDAFAVN
                     GADFFYTVPNHYVRTIKNEMAHNATPSGQLRSVAPGWTFWAVESMIDEIAHATGQDPA
                     QFRISLLDGKGKNDGGAQRLRNTLLAAMGLSGYGTRKLPKGEGIGVACVSSQERAAAS
                     WTACVAHVAVAPSGTVIVKKLTVATDVGTQVHPDNIRAQVEGAALWGLSLAMYEKATL
                     KDGGIEQTNFDTYTPLRMSQVPEVAVTVIANGEKPTGVGEPAVTVVAPAIGNAIFNAC
                     GARVRALPITAEAVKGAMKA"
BASE COUNT          560 a          822 c          875 g          474 t
ORIGIN      
        1 gtgcatatgg atgggaatct ctgccgctgc atgacctatt cgcggatcca gaaggcgatc
       61 atgcgggcgg caactgaaat gcgcaccgcc tcggtcggcg gcaatgagcg gaggcccaca
      121 tgaatcggca cgtgaaagtt accccccaga cgaccgatct cagtcgccgc tccttcctcg
      181 tcggcaccgc cgccgcaggc cttgtgcttg gctttgctgg cgtctccggg attgatcagg
      241 cctttgctgc ggcttccact aactttgagc ctagtgtttg gtacgcaatt tcacaggacg
      301 ggctcgtcac cgtgacctgc ggcaaggccg atatgggtca gcacatcgcc tccacgatgg
      361 cgcagctcat ctgcgaagag ctgggcgcca aatggaccga catgcgggtt caactcgcct
      421 ccaatgatcc gaagttcaac gaccccgtgc tgggtgcgca gatcaccggc gccagctggt
      481 cgacgatgat gaacttcgat gcgatgagcc gcgcgggcgc cgcgggacgc atggcgttga
      541 cggaagcggc ggcagccgcc atgagcgtgc cggcggctga gcttgtggtc cgcgattcca
      601 cgatctcgca tacgaagtcg aagaaacaga tgagctttgc cgacgtcgtc aagagcggca
      661 aggcgaccaa gaccttcacg ccggacgagc tcaaggcgat aaagctgaag actctcgatc
      721 agtacaccat gatcggcgta tcggtgccgc aactcgacat cccctccaag accgacggca
      781 cggccaagta tggcatcgat gtcatgctgc cgggcatggt ccatggcgcg atcgtcaccc
      841 cgccggtgca cttcggcgcg acggtgaagt tggtcgatga cagcagtgcc aagaaggtgc
      901 ccgggttcat caaggccgtc acccttgatg acaagactgg atcgacaacc ggctgggtcg
      961 tcgcggtcgc caatacttac gcgcaagcga agaaggcggc cgaggcgttg aagatcagtt
     1021 atgacggcgg cgcaaacgcg aagctgtcga gccagtcact gctcgatgag gcgaagcggc
     1081 tgcagggtct ttctgatgcc gggcaattct tcgtcaagga tggcgatccg gcggcggcgc
     1141 tcggaacggc ttcgaaggtg ttggaggcgg aatacaccac caacatcaac atccactgcc
     1201 cgatggagcc gatgaacgcg acggcagagt tcaagggcga catcctgcac atctattccg
     1261 gcaaccagtt cgcgacgcgc tccggcgcga tcgcggccgg cgccgccgga atcgatccca
     1321 agttcgtcgt catgcaccag atgtggctgg gcggcggctt tggccggcgt ctcgatgccg
     1381 acatgatggt gcccgcggtg caggcggcga aagccgtcgg caagccggtc aaggtgatct
     1441 actcgcgcga gaacgacatg accatggact actcgcgtcc gcttacgtac cagaaggtga
     1501 aggcaggcgt ggacggcgac ggcaagctga tcgcgattag ccatgacgtg gtttcggcct
     1561 ggccgacagc gcgctgggga atccccgatt tcctgacgcc ctcggtcgac aagaagggcc
     1621 cgctcgacgc gttcgcggtg aacggtgcgg acttcttcta taccgttcct aaccactatg
     1681 tacggacgat caagaacgag atggcgcaca acgccacccc gtcgggtcag ctgcgctcgg
     1741 tagcaccggg ctggacgttc tgggccgtcg aaagcatgat cgacgagatc gcacatgcga
     1801 ccggacagga ccccgcacag ttccgcatct cgctgctcga cggcaagggc aagaacgatg
     1861 gcggcgcgca gcggctgcgc aacacgctgc tcgccgcgat gggtctctcc ggctacggga
     1921 ccaggaagct gccgaaaggt gaaggcatag gcgtggcctg cgtgtcgtcg caggaacgcg
     1981 ccgctgccag ctggactgcc tgcgtcgccc atgtcgccgt cgcgccctcc ggcacggtga
     2041 tcgtgaagaa gctcacggtg gccaccgacg tcggaaccca ggtgcaccct gacaatatcc
     2101 gtgcccaggt tgagggtgcg gcgttatggg gtctgtcgct tgcgatgtac gagaaggcaa
     2161 cgctgaagga cggcggcatc gagcaaacca acttcgacac ctatacgcca ctccgtatga
     2221 gccaagtgcc ggaagtcgct gttacagtga tcgccaacgg cgagaagccg actggcgtgg
     2281 gcgagccggc ggtgaccgtg gtggcgccgg cgatcggtaa tgccatcttc aacgcctgcg
     2341 gcgcccgcgt ccgagcgttg ccgatcacgg cggaagctgt gaagggcgcg atgaaggctt
     2401 aaaggcggcc ccacattgcg acgcgagacg atccgccggg gaaactcccc ggcggttttt
     2461 tgtgcgcgac ttgcatagcc gggactgcta actgtaagat tcggctcaac taatgctata
     2521 tacacgaaac cgcgaaacgg cggtctaatg gggcccgtat gcatatgctc tcaagcggtt
     2581 gttggcagtt atcccggaag tcggtgttat gcaacgcgaa gtaagaagaa aaagcctgaa
     2641 cgctaaaagc accattgcgc cggtggaaat gccggggctc gacggccacc tttgcttcgc
     2701 gctctacgcc gccagcaatc acatgacacg t
//
LOCUS       DASWGM010000008         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_693652,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000008 DASWGM010000000
VERSION     DASWGM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693652"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /gene="dapB"
                     /locus_tag="VGA15_00215"
     CDS             <1..463
                     /gene="dapB"
                     /locus_tag="VGA15_00215"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX9586154.1"
                     /translation="AIVVKSGNMSLGVNLLAALVKRVAQSLDQDFDIEILEMHHRAKI
                     DAPSGTALMLGEAAAAGRGVALGQHSARGRDGQTGARRRGDIGFASLRGGSVTGDHSV
                     IFAGAMERIELTHRAEDRTMFAQGAIKAALWARGKKPGLYSMTDVLGLSDF"
     gene            486..1109
                     /locus_tag="VGA15_00220"
     CDS             486..1109
                     /locus_tag="VGA15_00220"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX9586155.1"
                     /translation="MSDRLLVLVRHGQSEWNLKNLFTGWKDPDLTEKGVAEAVDAGRK
                     LKAQGLSFDIAFTSVLTRAQHTLDLMLTELDQIGLPVKKHLALNERDYGDLSGLNKDD
                     ARKKWGEEQVHVWRRSYDVPPPGGESLKDTLARTLPYYVQEMLPCVLRGERTLVAAHG
                     NSLRALIMVLEKLSPKEILARELATGAPIIYRLNADATVASKVDLAT"
     gene            complement(1220..2116)
                     /locus_tag="VGA15_00225"
     CDS             complement(1220..2116)
                     /locus_tag="VGA15_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix domain-containing
                     protein/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9586156.1"
                     /translation="MMNLAMNEHRLTKPGPQNAALRDYDSVRRAIAFISEHWRAQPTI
                     EAMADAASVTPDELHHLFRRWAGLTPKAFMQALTLDHAKGLLRDSASVLDAALDSGLS
                     GPGRLHDLFVTHEAMSPGEWKNGGAGMTLRYGFHPSPFGTAIVIASGRGLAGLAFADP
                     GEEQAALADMQRRWPRATYVEDRDGTSALAQRIFDTKLWRADQPLRVVLIGTDFEVRV
                     WETLLKIPMGRAVCYSDIATKIKSPKASRAVGAAVGKNPVSFVVPCHRALGKGGALTG
                     YHWGITRKQAMLGWEAGQVGMH"
     gene            complement(2171..2692)
                     /locus_tag="VGA15_00230"
     CDS             complement(2171..2692)
                     /locus_tag="VGA15_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX9586157.1"
                     /translation="MTAGNDFDPEPEQPELFSALLTPHRSLNRTGFLVLMGFLSAVSF
                     AAGLAFLLMGAWPVFGFFGLDMLAIYWAFRVNFRDAKATEEIRVTPSELRVRRVSHRG
                     QVVEWVLNPLWVQLDQKTHAEFGIEKLYLVSKGRSISIARFLGADEKASFAKALNAAL
                     QAARRGPTYNPVL"
     gene            2715..>3328
                     /gene="nth"
                     /locus_tag="VGA15_00235"
     CDS             2715..>3328
                     /gene="nth"
                     /locus_tag="VGA15_00235"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9586158.1"
                     /translation="MAKITRGPRPESKLMAPKRAQKSRSRPAAKSGRAPAAKKPLKVL
                     TKSRASKSRASKSPASKSPASKPWTSAEVHEVFSRFRKANPEPKGELEHLNPYTLLVA
                     VVLSAQATDAGVNKATRALFAVADTPQKMLALGEEKVRDYIKTIGLYRNKAKNVIALS
                     EKLIAEFGGEVPRTRAEIESLPGAGRKTANVVLNMAFAEHTMAV"
BASE COUNT          670 a         1041 c         1055 g          562 t
ORIGIN      
        1 ggccatcgtg gtcaaatcgg gcaatatgag cctcggtgtc aacctgctgg cggcgctggt
       61 gaagcgggtg gcgcaatcgc tggatcagga tttcgatatc gaaattctgg agatgcatca
      121 cagggccaag atcgatgcgc cgtcgggcac ggcattgatg ctgggcgagg cggcggccgc
      181 gggccgcggc gtagcgctgg gccagcactc cgcgcgggga cgcgacggcc agaccggcgc
      241 gcgcagaagg ggcgatatcg gctttgcttc gctgcgcggc ggctccgtta ccggcgatca
      301 cagcgtgatt tttgcaggcg cgatggaacg tatcgagctg acgcaccggg ccgaggaccg
      361 gaccatgttc gcgcagggcg cgatcaaggc agcactttgg gcgcgcggca agaagcccgg
      421 actgtattcg atgaccgacg tgcttgggct cagcgacttc tagaatcgaa acggaaatca
      481 agagaatgag cgaccgtctt ctcgtgctcg tgcgtcacgg ccagagcgaa tggaacctga
      541 aaaatctttt caccggctgg aaggatccgg atctcaccga gaaaggcgtc gcagaggccg
      601 tcgatgccgg gcgcaagctg aaggcgcagg ggctgtcatt cgacatcgcg tttacctcgg
      661 tgctgacgcg cgcgcagcac acgcttgatc tgatgctgac cgagttggac cagatcggtc
      721 ttccggtcaa aaagcatctg gcgctgaatg agcgcgacta cggcgatctg tcgggtctga
      781 acaaggacga cgcccgcaag aaatggggcg aggagcaggt gcatgtctgg cggcgctcct
      841 acgacgtgcc accgcccggc ggcgaaagcc tgaaggatac attggcgcgc accctgccct
      901 attacgttca ggagatgctt ccctgcgtgc tgcgcggcga gcgcacgctg gttgcagccc
      961 acggcaattc actgcgcgcg ctgatcatgg tgctggaaaa actttcgccg aaagaaattc
     1021 tcgcgcgcga actcgccacc ggtgcgccga tcatctaccg cctgaacgcc gacgccaccg
     1081 tggcgtccaa ggtcgatctg gctacgtgag cgcagcttgt agagccgtag gatgggttga
     1141 gcgaagcgaa acccgtcagc gacaacaacg aggatgatgg gtttcgcttc gctcaaccca
     1201 tcctacgaac taacggtcat caatgcatcc ccacctgccc ggcttcccag cccagcatcg
     1261 cctgcttgcg ggtgatgccc cagtggtagc cggtcagcgc gccgcctttg ccgagcgcac
     1321 gatggcacgg caccacgaac gacaccggat tttttccgac cgcggcgccg accgcgcgcg
     1381 aggccttggg gctcttgatc ttggtcgcga tatccgaata gcagaccgcg cggcccatcg
     1441 ggattttcaa gagcgtctcc catacccgca cctcgaagtc ggtgccgatc aggaccacgc
     1501 gcaacggctg gtccgcgcgc catagtttgg tatcgaagat gcgctgcgcc agcgccgagg
     1561 tgccatcgcg atcctcgaca tatgtcgcgc gcggccagcg ccgctgcatg tcggcgaggg
     1621 ctgcctgctc ctcgccggga tcggcgaaag caagaccggc gaggccacgg ccgctggcga
     1681 tcacgatggc agtgccgaat ggcgagggat ggaacccgta gcgcagcgtc atgccggcgc
     1741 cgccgttctt ccactcgccc ggcgacatcg cctcatgggt gacgaataga tcgtgcagcc
     1801 ttcccgggcc cgaaagcccg gaatcgagcg cggcatcgag cacgctggcg gaatcgcgca
     1861 acaggccctt ggcgtgatcg agggtcagcg cctgcatgaa ggcctttggt gtcagtccgg
     1921 cccagcggcg aaacaaatgg tgcagctcat cgggcgtgac gctggccgca tccgccatcg
     1981 cttcgatggt cggctgcgcg cgccagtgct ccgagatgaa ggcgatagcc cgacgcaccg
     2041 aatcatagtc gcggagcgcg gcgttctggg ggcccggttt ggtgaggcgg tgctcattca
     2101 tggcgaggtt catcatgggt ctgatgtagg ccgggcgcgg tgtccaaacc acccgattcc
     2161 cgacagcttg tcagaggacg ggattgtagg ttgggccgcg ccgcgcggcc tgcagcgcgg
     2221 cattcaaggc cttggcaaag ctagcctttt cgtcggcgcc caaaaatcgc gcgatcgaga
     2281 tgctgcggcc tttggacacc agataaagct tttcgatgcc gaattcggcg tgggttttct
     2341 gatcgagctg gacccacaag ggattgagca cccattcgac cacttgcccg cgatggctca
     2401 cccgacgcac gcgcagctcg gatggcgtca cccggatttc ctcggtggcc ttggcgtcgc
     2461 ggaaattgac ccggaacgcc cagtagatcg cgagcatatc gaggccgaaa aagccgaaca
     2521 ccggccaggc gcccatcagc agaaacgcca gcccggcggc aaagctgacc gcgctgagaa
     2581 accccatcag caccagaaag ccggtacggt tcagcgagcg gtgcggcgtc agcagcgccg
     2641 aaaacaattc gggttgctct ggctccggat caaagtcgtt gcctgcggtc atcgcgtatc
     2701 agtatatccc gatcatggcg aaaatcaccc gcggaccccg gcccgaatcg aaactcatgg
     2761 ctcccaaacg cgcccaaaaa tctcgcagtc gtcccgcggc caaatcgggg cgcgcgccgg
     2821 cggcgaaaaa accgctcaag gttttgacca aatccagggc ttccaaatcc agggcttcca
     2881 aatctccggc ttccaagtct ccggcttcca aaccctggac atcagccgaa gtccacgaag
     2941 tgttcagccg gtttcgcaag gccaatcccg agccgaaagg agagctggaa catctcaacc
     3001 cctatacgct gctggtggcc gtcgttttgt ccgcgcaggc gaccgacgcc ggcgtcaaca
     3061 aagcgacccg cgcgctgttc gcggttgccg acacgccgca aaagatgctc gcgctcggcg
     3121 aggaaaaagt ccgcgactac atcaagacca tcgggctcta tcgcaacaag gcgaagaacg
     3181 tcatcgcgct gtcggaaaaa ctgatcgccg agttcggcgg tgaggtgccg cgtacccgcg
     3241 ctgaaatcga atcgctgccc ggcgccggcc gcaagaccgc caatgtcgtg ctcaacatgg
     3301 ctttcgcaga acataccatg gcggtgga
//
LOCUS       DASWGM010000009         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1940900,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000009 DASWGM010000000
VERSION     DASWGM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940900"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..587)
                     /locus_tag="VGA15_00240"
     CDS             complement(30..587)
                     /locus_tag="VGA15_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9586159.1"
                     /translation="MTETTAKKKLPAAVERFVLHWGDMGDEWGVNRSVSQIHGLLYLS
                     EAPMTADDIADTLGMARSNVSNSIRELLSWNLIRRVPIMGDRRDHYEAETDIWEVAAR
                     IAAGRKEREIDPAVDALRACVVDAADDPTISPVAIKRLKEMLAFTELVDRWYAQMLTV
                     PRPRLIALIKLGEKIVSFLPAGKSK"
     gene            803..1087
                     /locus_tag="VGA15_00245"
     CDS             803..1087
                     /locus_tag="VGA15_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586160.1"
                     /translation="MRVLCAAAVIALLAGPAYAQQKSVPQYGDAPADKTRSEIEQDRT
                     AERAYKRSLGNIPDQTPTDPWGNARSADAPKAVAKPSPAKPRTKTGATAN"
     gene            complement(1136..1435)
                     /locus_tag="VGA15_00250"
     CDS             complement(1136..1435)
                     /locus_tag="VGA15_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX9586161.1"
                     /translation="MSGTIRQVTIRGRVQGVGYRYWVERQAVAHGLEGWVRNRPDGSV
                     EALFSGPEDVVSNMVASCRRGPSSARVDAVDERPGDADALNLRRPGERFSVLPTI"
     gene            complement(1436..2302)
                     /locus_tag="VGA15_00255"
     CDS             complement(1436..2302)
                     /locus_tag="VGA15_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX9586162.1"
                     /translation="MAFRKCREALRSILAGSTCIRPGSVYDAISIRIAEDLGFEVGMF
                     GGSVASLAVLGDPDIALITLTELAEQMRRMSRAAALPVLVDADHGYGNALNVRRTVQE
                     LETAGAAGLTIEDTLLPQSFGQAKTQLISLEEGVGKMKAALDGRGDPSLVIMARTGAA
                     SVSGLEDAIARARAYEATGVDALFFTGLKTRAELEAIAAATTLPIVLGGFPEEAADPN
                     YLASKRVRIALQGHAPIAAATLAVYEMLKALRDGQSPKNLKGLASSDLTDRVTRDADM
                     KARAAEFLGLKR"
     gene            complement(2372..>3226)
                     /locus_tag="VGA15_00260"
     CDS             complement(2372..>3226)
                     /locus_tag="VGA15_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9586163.1"
                     /translation="DPMIAKLVTHAPSRAAAIEAQATALDSFYIDGIRHNIPFLSALM
                     LHPRWREGNLSTGFIAEEFPKGFAVRVPEGEVARRLAAVAAAVDHVLGERKRQISGQL
                     IHRPVQRERRRAVWLDRNEIALDIAREDDFITVRFVGADGKPGHPHQLVSPWVPGDLV
                     WQGSVDGHPVAMQVRPIPNGFRLAHQGFEVAVNVFTESEAAAARLMPVKAPTDTGKKL
                     LCPMPGLVVSIAVAEGQEVKSGETLAVVEAMKMQNVLRAERDGTVKKIHATAGATLAV
                     DALILEFA"
BASE COUNT          628 a         1090 c          951 g          557 t
ORIGIN      
        1 gccaccatgc actccagcaa tctcaaacct tatttggatt tcccggccgg caagaagctg
       61 acgatctttt cgccgagctt gatcagggca atcaaccttg gccgcggcac ggtcagcatc
      121 tgcgcgtacc agcgatcgac cagttcggtg aaagccagca tttccttcag ccgcttgatc
      181 gcaactggac tgatcgtcgg atcgtcggcg gcgtcgacga cgcaggcgcg cagcgcatcg
      241 acggcgggat cgatctcacg ctccttgcga ccggcggcaa tgcgcgcggc cacctcccag
      301 atatccgtct ccgcctcata gtgatcgcgg cgatcgccca tgatcggaac gcgccggatc
      361 aaattccagg acaacaattc cctgatggaa ttggagacgt tggatcgcgc catgcccagc
      421 gtgtccgcga tatcgtcagc ggtcatcggg gcttccgaca aataaagcag accgtggatc
      481 tggctgaccg agcgattgac cccccactca tcgcccatgt cgccccaatg cagaacgaac
      541 cgttcgacgg cggcgggcag tttcttcttg gctgttgttt ctgtcataac agaaatatct
      601 gacgagagca gacgaatgtc aatggaaatt ctcgagacag acacgccttg gtcctgaata
      661 aggaccgttg ccagtccatc gcctgccggc ggccttgccc ggccgcgcaa tgtcactaga
      721 actgatgttg gggatggcgg cccgcgccgc tctgcacgcg gcgatccgcg tcgcgctcag
      781 aacaccgtcg agggagagat gcatgagagt tttgtgcgcc gctgctgtca ttgcgctgct
      841 ggccgggcct gcttacgccc aacaaaagtc ggtaccccaa tacggggatg ctccagctga
      901 caaaacccgg agcgagatcg agcaggatcg gacggccgag cgggcctata agcggtccct
      961 cggcaacatt cccgaccaga cgcccaccga cccctggggc aacgcgcgca gcgcggatgc
     1021 gcccaaggcc gtcgccaagc cctctccggc gaaaccgcgg accaaaaccg gcgccaccgc
     1081 aaattgaagc acgatcccgg atcaagtccg gggcggactt tcgcttgaaa acgctttaaa
     1141 tcgtcggcag caccgaaaat cgctcgccgg gccgccgcaa attcagcgca tcggcatcgc
     1201 cggggcgctc atcgactgca tcgacgcgcg ccgatgacgg gccgcggcgg cacgacgcca
     1261 ccatgttcga cacgacatcc tccggtcccg aaaacagcgc ctcgacgctg ccatccgggc
     1321 ggttgcgcac ccatccctca agtccgtgcg ccaccgcctg gcgctcaacc caataccgat
     1381 agccgacacc ctgcaccctg ccccggatcg tcacctgacg aatcgtgccg ctcatctagc
     1441 gcttgagtcc caggaattcg gcggcgcgcg ctttcatgtc tgcgtcgcgc gtgacgcggt
     1501 cggtcaggtc ggatgacgca aggcccttca aattcttcgg cgactggccg tcgcgcagcg
     1561 ccttcagcat ctcgtaaacg gccaaggtcg ccgcggcaat tggggcgtga ccctgcaacg
     1621 cgatgcgcac ccttttgctg gcgagatagt tcgggtcggc cgcttcctca gggaagccac
     1681 ccagcacgat cggcagcgtc gtcgcggcag cgatggcttc gagttccgcg cgggttttca
     1741 ggccggtgaa gaacaatgca tcgacgccgg tggcctcata ggcccgggcg cgcgcgatcg
     1801 catcctcgag acctgagacc gacgcagcgc cggtgcgcgc catgatcacc aacgatggat
     1861 caccgcgtcc gtccagcgcg gctttcatct tgccgacacc ttcctccagc gaaataagct
     1921 gcgttttggc ctgcccaaag gactgcggca gcagggtgtc ctcgatggtg aggccggcgg
     1981 cgccggccgt ttccagttcc tgaaccgtgc ggcggacatt cagcgcattg ccatagccgt
     2041 gatcggcatc gaccagtacc ggcaacgcgg cagcgcgcga catccggcgc atctgctcgg
     2101 caagttcggt cagcgttatc agtgcgatat cgggatcccc tagcaccgcg agcgaagcca
     2161 ccgatccgcc gaacatgccg acctcgaaac cgagatcctc ggcgatgcgg atcgaaatcg
     2221 cgtcatagac cgaccccggc cgaatgcagg tcgatcccgc caggatcgaa cgcaacgctt
     2281 cgcggcactt tcggaaagcc atgcctgccc ctctcataat tgcgcgtatt ctggtcgcaa
     2341 aaccggtatc cacttttgcg gaatacgcgg cttaagcaaa ttcgagaatg agcgcatcca
     2401 ccgccagcgt tgcgccggcg gttgcgtgaa tcttcttgac cgtgccgtcg cgttcggcgc
     2461 gcagcacatt ctgcatcttc atcgcctcga ccaccgccag cgtttcgcct gatttgacct
     2521 cctggccttc ggcgaccgca atcgacacca cgagccccgg catcggacac agcagcttct
     2581 tgccggtatc ggtcggggcc ttgaccggca tcagccgcgc cgcggcggcc tcggactcgg
     2641 tgaagacatt gaccgccact tcaaaaccct gatgcgcgag gcggaagcca ttgggaatcg
     2701 ggcgcacctg catggccacc ggatggccgt cgacgctgcc ctgccagacc agatcgcccg
     2761 gcacccaggg cgagaccagc tgatgcggat ggcccggttt tccgtccgcg ccgacaaaac
     2821 gcactgtaat aaaatcgtct tcccgcgcaa tatcgagcgc gatttcgttg cggtccagcc
     2881 acaccgcgcg gcggcgctcg cgctgcaccg gacggtggat cagctggccc gaaatctgcc
     2941 gcttgcgctc tccgagcacg tgatcgacgg cggccgcgac cgcagctaac cgacgggcaa
     3001 cctcgccttc cgggacacga accgcaaatc ccttcggaaa ctcctcggca atgaagccgg
     3061 tggatagatt gccctcgcgc cagcgcggat gaagcatcaa cgccgacaga aacggaatgt
     3121 tatggcggat accgtcgatg tagaatgaat cgagcgcggt cgcctgcgcc tcgatcgcgg
     3181 cggcgcgcga cggcgcgtgg gtcacgagct tggcgatcat cggatc
//
LOCUS       DASWGM010000010        11165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_832117,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000010 DASWGM010000000
VERSION     DASWGM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11165
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832117"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VGA15_00265"
     CDS             <1..367
                     /locus_tag="VGA15_00265"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9586164.1"
                     /translation="LGSEAIRVGQAHGYQLEEVLHLPPETIARAGEGDEAAMRACDEQ
                     RFKDGKHTSSEQRPSMGQDMQKGRRTEIEFLNGFVVREGKKVGLSCSANAVLTDIVKR
                     VERNELSPDPRHITELRLN"
     gene            450..1268
                     /locus_tag="VGA15_00270"
     CDS             450..1268
                     /locus_tag="VGA15_00270"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9586165.1"
                     /translation="MTGQDSSDWLGLSGRVCVVTGGGGGIGRAVASSLARAGAHVAAI
                     DRDERGLETTRAELRELGCEHVIASCDTSNAESVVAASETTLKSLGSCSVLVNAAAVL
                     RPGGLENLSLAEWNAVLSVNLTGYFLCAQIFGRHMRKLGRGSLIHVASIAGSHAQAHS
                     GAYSVSKAGVVMLSRQLASEWGPHGIRSNVVSPGMIITPMSQSFYDTPGVTERRTAVV
                     PTRRIGMPQDIADAILFLASDRSSYVNGDEITVDGGYANMLMNLVPRPGFEKPS"
     gene            complement(2118..3071)
                     /locus_tag="VGA15_00275"
     CDS             complement(2118..3071)
                     /locus_tag="VGA15_00275"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028174674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX9586166.1"
                     /translation="MPAKNGSIKLVAGNSNPVLAQAISDWLHLPLTKAVVRRFADMEI
                     FVEIQENVRGSDVFIIQSTSYPANDHLMELLIITDALRRASARRITAVIPYFGYARQD
                     RKVGSRSPISAKLVANMITHAGVDRVMTLDLHAAQIQGFFDIPTDNLYASPVIVRDIR
                     ERFDLTKVMVVSPDVGGVVRARGLAKRINTPLAIIDKRRERAGESEVMNVIGDVDGHT
                     CILVDDMVDSGGTLVNAADALIANGAKEVSAYISHGVLSGGAAARITSSKLKELVITD
                     SIQPTEAVRNAANIRTLSIASLIAEAIGRTASEESVSSLFD"
     gene            complement(3240..3809)
                     /locus_tag="VGA15_00280"
     CDS             complement(3240..3809)
                     /locus_tag="VGA15_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586167.1"
                     /translation="MLLAMACGLGGCAGGGAASGAYAMAGSSGGATVAFESIDGPPPQ
                     VFDRMVSVLDSESKLRNLSIVSREGASAYRVRSYLSVQVNHGRASIAWVWDVYDRDQQ
                     RALRLAGEEPAGKTAGRDAWAAADDLVLRKIAQAGLSGLSGMINGTLPADTPPAAAPG
                     KDGPAVASTEQPLPASGAFSVSALSYTAR"
     gene            complement(3999..5113)
                     /locus_tag="VGA15_00285"
                     /pseudo
     CDS             complement(3999..5113)
                     /locus_tag="VGA15_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
     gene            complement(5234..7042)
                     /gene="yidC"
                     /locus_tag="VGA15_00290"
     CDS             complement(5234..7042)
                     /gene="yidC"
                     /locus_tag="VGA15_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9586168.1"
                     /translation="MHDQNNVFYAVALSLVILITWQYFFATSFLGRPSAQKTSQIGMM
                     APDAAIRPQPQAIAPQAATIPSPQRSQPFSRQDALARSQRVAVDTPRVKGSIALTGGR
                     IDDLSLVQYRETTDPKSPAIQLLSPSGGPQPFYAEFGWINSSGINLQLPTPETKWRLE
                     GSDSLGIGRPVRLVWQNGEGLEFRRTISVDDKYLFNVRDEVANKGQAAVVLAPYALIS
                     RHSPPPTLGYYLLHEGAIGVLGDQGLQEVTYKTLDDKKRMAFNIGHAWLGFTDKYWAA
                     ILLPETSSQLQAEFSASSLGALKTYQADYVQDAQTIEPGGTATATARLFAGAKEVAIL
                     DGYDKALNLDRFDLAIDWGWFRFITKPMFTLIDAIFRWVGNFGVAILAVTLMLKIAFF
                     PLANKAYASAAKMKALQPQLQSIRESFGNDKVKQQQATLELYRKEKINPIAGCLPTLV
                     QIPVFFALYKVLFVTIEMRQAPFFGWIRDLSAPDPTNLFNLFGLIPYDPVSLPLLGGF
                     LALGAWPLLMGFTQWVQMKLAPASPDPAQSAVLNWMPVIFTFMLAKFSAGLVIYWTWN
                     NSLSIIQQGIVMRRNGVRIELWDKVRAMFGKRKAGD"
     gene            7327..7929
                     /locus_tag="VGA15_00295"
     CDS             7327..7929
                     /locus_tag="VGA15_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586169.1"
                     /translation="MKFEFFIHDRRVRPWGQALVAARSWCCDERCTTDVADLPRFELG
                     NMNMAIAGRAPSGDSASRGVRSGTGGFGALSAHAAKLLALAALSLTMALAGCAQNSAQ
                     HEVKTDPIEAAAPPQGHPDFRIRRPNRALLLSQPAPDCEFRSTELKTVDPDQWGRLRL
                     DYERQCYQQAEKKVRDRLRLLQASSRCEIEPVRTPLATIR"
     gene            8221..9078
                     /locus_tag="VGA15_00300"
     CDS             8221..9078
                     /locus_tag="VGA15_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9586170.1"
                     /translation="MKTLYDLLGALPDDDAESLRTAFRQAAKATHPDTNTDDPDAPLK
                     FRQIVRANAILSDADQRATYDRLLALTLRQRHWKLKRTIRKVASDAIKVACLSAVSIG
                     GYLVFEQVSKASIVPVKPTEIAARARTEIAAVTPTKQSDTTGRDKPPEKLESAGVASD
                     AIVPSGAAPATNAAPAANTDSTQAMANADPAPDLTARDAKSYRERGIAAYIGGDLYRA
                     LADFDVAIQIDPGFADAYIDRGIVFYRIHEFDRAFADIAQAKRVKNSSRTKTLPLAPR
                     KVSSSSAKN"
     gene            complement(9135..9905)
                     /gene="pgeF"
                     /locus_tag="VGA15_00305"
     CDS             complement(9135..9905)
                     /gene="pgeF"
                     /locus_tag="VGA15_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX9586171.1"
                     /translation="MRLGSPLLSAIPGLRHTFFTREGGVSGGIYASLNGGLGSRDDPA
                     SVAENRRRMAEQIGVIPEHFLSVHQTHSPNVVVASGPWQGVSRPLADAIVTRNEGLAI
                     GATAADCGPILLVDPSARVIGAAHAGWKGALTGIVEPTVEAMEQLGAERSGIVAAIGP
                     LIRQHSYEVGSEFVERFVQADAENTLFFIPAAREGHSMFDLAGFIRMRLENAGVLMID
                     DTGIDTYSDERFFSYRRSVHRGEPDYGRHVHTIALASE"
     gene            complement(9902..10972)
                     /locus_tag="VGA15_00310"
     CDS             complement(9902..10972)
                     /locus_tag="VGA15_00310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9586172.1"
                     /translation="MPVWRYMELCLSHPQHGYYVSRDPLGREGDFITSPEVSQMFGEL
                     LGLWAASVWKAIGSPSLLRLIELGPGRGTMMADALRALRVLPPLYQALSIHLVEINPV
                     LREKQKAALSGVRVIAWHNSIDEVPKGPAVIFANEYFDVLPIHQMVKRETGWHERTVE
                     LDGNGKLAFGFAAEPTPRFEVLLPPLVRAAPVGAVFEWRPDAEIMKIATRVRDQQGAA
                     LIIDYGHVRSDAGDTFQAIARHSFADPLKNPGLADVTAHVDFQALVRAVEDLGARAHG
                     PVPQGEFLKRLGIETRALTLMAKTTHEISEDISSALKRLTGDGRGGMGSMFKVLAISE
                     PGLASLAGLNDQDQQDVGAELP"
     gene            complement(11029..>11165)
                     /locus_tag="VGA15_00315"
     CDS             complement(11029..>11165)
                     /locus_tag="VGA15_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX9586173.1"
                     /translation="FLWGGLTMGMLLSVPMIIAGAIIIVVAWRRTMSNQMPNPIEGKP
                     "
BASE COUNT         2160 a         3426 c         3512 g         2067 t
ORIGIN      
        1 gctcgggagc gaagcgatcc gcgtcgggca agcgcacggc tatcaactgg aggaggtttt
       61 gcatctgccg cccgagacga ttgcgcgtgc gggcgagggc gacgaggcgg cgatgcgcgc
      121 ctgcgacgag cagcgcttca aagacggcaa gcacacctcc tccgagcagc gtccttccat
      181 gggccaggac atgcagaagg gccggcgcac ggagatcgaa ttcctcaacg gcttcgtggt
      241 gcgcgagggc aagaaggtcg gcctctcgtg cagcgccaat gccgtgctga cggacatcgt
      301 caagcgcgtg gaacgtaatg agctgagccc ggatcctcgg cacatcactg agctgcggct
      361 gaactgagcc ggcttgcgcg aatgcgatcc cacatgcgtt cagctatgct atcgggcgtc
      421 acctcactgg attgggacag tagactgata tgaccggaca agactcatcc gactggcttg
      481 gcctgtcagg ccgcgtttgc gtggtgacgg gcggaggtgg cggcatcggc cgggccgtcg
      541 cgtcaagtct cgcccgggcc ggcgcccatg tcgccgccat cgatcgcgac gaacgcggcc
      601 tcgagacgac gcgcgcggag ctgcgcgaac ttggctgcga gcatgtgatc gcaagctgtg
      661 acacctcgaa cgccgagagt gttgtcgccg cctccgagac gaccctgaag tcgctgggat
      721 cgtgcagcgt gctggtcaac gcggcggcgg tgttgcgtcc cggcgggctc gaaaatctgt
      781 cgcttgccga atggaacgcc gttctttccg tcaacctgac cggctacttc ctctgcgccc
      841 agatctttgg ccggcacatg cgaaagctcg ggcgcggcag tctcattcat gtggcgtcga
      901 tcgcgggcag ccatgcccag gcgcacagcg gcgcctacag tgtcagcaag gccggtgtcg
      961 tcatgctctc gcgtcagctc gcgagcgaat ggggaccgca cggcatccgc agcaacgtcg
     1021 tcagtcccgg catgatcatc acgccgatga gccagtcctt ttacgacacg cccggggtta
     1081 ccgagcggcg caccgctgtc gtcccgacgc gacggatcgg catgccgcaa gacatcgcgg
     1141 atgccatcct gttcctggcc agcgaccggt cgtcctatgt gaacggcgac gagatcacgg
     1201 tggatggcgg ctacgccaat atgctgatga acctggtgcc gcggccgggg tttgagaagc
     1261 cgagttgaag tcgcagtatc cgggctgccg atcggccacc tgagcaccca agtctgttgc
     1321 gcctgcgcgg cgatttgttg tgctttctca atgtgatttg ggctgtccag tcctccttgc
     1381 aaaaatattt cacttcaccc gtcgggcaaa tcatctctac aaattcgcgc catcccgtcc
     1441 cacacagagg ggcgtttcgc gatcgtcacg gacgtagggc acgggatgcg gtggacgcgg
     1501 cagcgttttg cgcgcgatgg gatcgcaggg cgagtcgaaa gacttgtgag cggtcgccgg
     1561 cacgctgacg agagatgttg ctgcggacgg caaaatcgtg tggtcctgac gccccgacgc
     1621 tggcgtcaag ttcgcggagt tgcgtcggcc cgaccgggct tagacaagac catatccgct
     1681 gacgacggtg gcaaaagagc ccggtcaccg gggagagccc gacataagcc gtaaaaccat
     1741 tgcgtgcggg aatgtcgggt gattccggtg tactcgttgt tactcgtgtg cgttccacca
     1801 atacaaaggg cacacgaggc cgcgggtgca gcgggcaccc ggcattcccc acgccctcca
     1861 agggggcgag agataaatgc acgactcggg cgcattcgcg ctgcgggacg cgaacgcgcg
     1921 tctggatttg gcgcgttatg tcgaagctca tcatggccgt gggacaagcc cggccatgac
     1981 aaaaaatgct cacacaggat ggaaattaga aacccgtagc ccggatggag cgtagcgaaa
     2041 tccgggacat cacgtaacga gagccccgga ttccgcttcg ctccatccgg gctacatagc
     2101 gataggctcg cttgattcta atcaaacaaa ctcgacaccg actcttccga tgcggtgcgg
     2161 ccgatggcct ccgcgatcag cgaggcgatc gacagcgtgc ggatgttggc ggcgttgcgt
     2221 accgcttcgg tcggctggat cgagtcggtg atgacaagct ctttcagctt ggagctggtg
     2281 atgcgggcgg cggcgccgcc ggacagcacg ccgtggctga tataggcgga aacttccttg
     2341 gcgccgttgg cgatcagggc gtcggcggcg ttcaccagtg tgccgccgga atcgaccatg
     2401 tcgtcgacca ggatgcaggt atgtccgtcg acatcgccga tcacgttcat gacttcggat
     2461 tcgccggcgc gttcgcggcg tttgtcgatg atcgccagag gcgtgttgat gcgcttggcg
     2521 aggccgcgcg cgcgcaccac gccgccgaca tcgggcgaca ccaccatgac cttggtgagg
     2581 tcgaagcgtt ccctgatgtc gcgcactatc accggcgagg cgtagagatt atcggtcgga
     2641 atgtcgaaga agccctggat ttgcgctgcg tgcaggtcga gcgtcatcac gcggtcgacg
     2701 ccggcgtggg tgatcatgtt ggccaccagc ttggcggaaa tcggcgagcg cgacccaacc
     2761 ttgcggtcct gccgggcgta gccgaaatag ggaatcaccg cggtgatgcg ccgcgccgaa
     2821 gcccggcgca gcgcgtcggt gatgatcagc aactccatca gatggtcgtt ggccggatac
     2881 gacgtcgact ggatgatgaa cacgtccgag ccgcggacat tttcctggat ttcgacgaag
     2941 atttccatgt cggcgaagcg gcggaccacg gccttggtca gcggcagatg cagccaatcg
     3001 gagattgcct gcgccaggac agggttggaa ttgccggcga cgagcttgat ggagccattc
     3061 ttggccggca tcgacccttc tccccgcgcc ttgctggata cgatgttcag catatcggga
     3121 tacccacaaa aaaggcgcgt ttcgacgagc gaggtaatat caagcagatg gctcggctgg
     3181 caacccgtcc taccaggttt tcctgcgcaa aatcgctggt ttggcaacta tttgtatcgt
     3241 taacgcgcgg tgtaactcag cgcgctgacg ctgaatgcac ccgaggcggg caagggctgt
     3301 tctgtgctcg ccaccgccgg gccgtccttg cccggcgcgg cggcgggcgg cgtgtctgcc
     3361 ggcagtgttc cgttgatcat gccgctgagg ccgctcaatc cggcctgcgc gattttccgc
     3421 aacaccagat cgtcggccgc ggcccaggcg tcgcgtcccg cggtcttgcc ggccggctcc
     3481 tcgccggcga ggcgcagcgc gcgctgttgg tcgcggtcat agacgtccca gacccaggcg
     3541 atgctggcgc ggccgtgatt gacctggacc gaaagatagc ttcgcacgcg ataggccgat
     3601 gcgccttcgc gcgagacaat cgacaggttg cgcagcttcg attcgctgtc gagcacactc
     3661 accatccggt cgaatacctg cggcggcggg ccgtcgatcg attcgaacgc taccgtggca
     3721 ccgccgctgc tgccggccat cgcataggcg ccgctcgcgg cgccgccgcc ggcgcagccg
     3781 ccgagtccgc acgccattgc cagcagcatg gccgcaaccg cggcgcgcgg tgcggtccgg
     3841 gtgcccgcga cggcccggaa cagggttgcg atggtgtccc tcatcgaggg gagcgatatc
     3901 gttaaaatgt gttaaggtct agggcaggga gccgtcacat ttcgaaaaga ttgaatttcg
     3961 agcggtcctt tttcgatctc aattatttga atgccttgtc actccgagct cagcgctcgg
     4021 cgccgcgcat gacgcgcaaa cgcatgcgcg atcagtctgt ccatcagatc ggtttgggac
     4081 aatccgctgg cttcccagag cttgggatac atgctgatcg aggtgaagcc cgggagtgtg
     4141 ttcacttcgt tgacaaacat gtcgtcgcct ttgacgaaga aatctattcg ggcgaggccc
     4201 gcgcagccga gcagccgaaa cgcctcaacg gccagctcct gcagacgacg gacctgctgc
     4261 ggggaaagtt ttgcgggaat cagcagggcg gcaccatccg cgtcgatata tttggctgat
     4321 acgaatagaa gccgtgctcg ctcgccggca cgatctcgcc gagttgcgat gcatgaatgt
     4381 ggccgtcgga ttcttccagg acagaacatt caatctcccg cgcctcgctc accgcctttt
     4441 cgaccaggat cttatggtcg tagcggagtg cgagttcgca cgacgccgcg aattcctcgc
     4501 gcgagcgtgc gcgcgagaca ccgaccgacg aacccatatt ggcaggcttt acaaacaaat
     4561 cgctcgttcc cagcgcggca acggccgtag cgtagtcgat gggagacaaa gacgttgcgg
     4621 tgacgaacgg caccacggga aggccggcat cacgcatcag gcgcttggcg acatccttgt
     4681 ccatcgcgat ggccgaaccg gtcaccgccg aacccacgaa cggcacgtcc gccagctcaa
     4741 gcgcgccttg cacggttccg tcttctccgt tcggcccgtg caggaccggg aatacaacgt
     4801 cgaagcgcgt tacggcgctg cgtctcggtt ggtcgctgtc gcaccacacg aaactgcctc
     4861 cgccgcctgg aatcagcgcg atttgcggcg cgtcgtcggg gattaccagc gcggcggcgc
     4921 ccgttcccgc gccgttaccg gcatcgcaga tacgccagcg accatccttc gcgatgccga
     4981 tcagggaaac ctcataacgg tcggggttca gggcgcgaag gatgttggcg gcagaggctc
     5041 tggagacttc atgttcatcc gagcggccac cgaacaccag cccgacagtg atcttgcgac
     5101 gctctccagc catcattcca ccgtgtgcca ttgggccgtc atgacagccg tcagctcgaa
     5161 ccgaggtcgc agattaagac tcaatggctg gcaaaatgca aagccgggcc acagcggcga
     5221 tcggatcgcc gctctaatcg ccggctttgc gttttccgaa catcgcccgg actttgtccc
     5281 agagttcgat ccggacacca ttcctgcgca tgacgatgcc ctgctgaatg atcgaaaggg
     5341 aattattcca ggtccaatag atcaccaggc ctgccgagaa cttggccagc atgaaggtga
     5401 agataaccgg catccagttc aggacagccg attgcgccgg atcgggcgac gccggcgcga
     5461 gcttcatctg cacccactgc gtgaagccca tcagcagcgg ccaggctccg agcgcaagaa
     5521 acccgccaag cagaggcaga gacaccgggt cgtacgggat cagcccgaaa agattgaaca
     5581 ggttggtggg gtcgggggcg gacagatccc tgatccagcc gaagaaaggc gcctgacgca
     5641 tctcgatggt gacaaacagg actttgtaga gcgcgaagaa aaccggaatc tgaaccagcg
     5701 tcggcaggca cccggcgatc gggttgatct tctccttcct gtaaagctcc agtgtcgcct
     5761 gttgttgctt caccttgtcg ttgccaaaac tctcgcggat agactgaagc tgcggctgca
     5821 gggccttcat cttcgccgcc gacgcatagg ctttgttggc gagcggaaag aaggcgatct
     5881 tgagcatcaa ggtgacggcc aggatcgcca cgccaaagtt tccgacccat cgaaagatgg
     5941 catcgatcaa ggtgaacatc ggctttgtga tgaagcggaa ccagccccag tcgatcgcaa
     6001 ggtcgaaccg atcgagattg agcgctttgt cgtagccgtc gaggatcgca acttccttgg
     6061 cgccggcgaa cagccgggcg gtggcggttg cggtgccacc gggctcaatg gtctgtgcat
     6121 cctgcacata gtcggcctga taggtcttga gcgcgccgag ggagcttgcg gagaattctg
     6181 cctggagctg cgaagacgtc tcggggagca agatggccgc ccagtatttg tcggtaaaac
     6241 cgagccacgc atggccgata ttgaacgcca tccgcttttt gtcatcgagg gtcttgtagg
     6301 taacttcctg caatccctgg tcgcccagaa ccccgatggc gccttcgtga agcagataat
     6361 agccaagcgt cggtggcgga ctgtgacggg agatcaatgc atagggagcc agaacgacgg
     6421 ccgcctggcc tttgttggcg acctcgtcgc ggacgttgaa gaggtatttg tcgtcgaccg
     6481 aaatcgttcg ccgaaattcc agcccctcgc cattttgcca aaccagcctc accgggcgtc
     6541 caatcccaag gctgtcggat ccctccagac gccatttggt ttcgggagtg ggcagctgca
     6601 gattgatgcc ggaagaattg atccagccga actccgcata gaatggttgc gggccgcccg
     6661 atggcgaaag cagctggatg gccggcgatt tgggatcggt ggtttcgcga tattgcacca
     6721 gcgacaaatc gtcgatccgg ccgccggtca acgcaatcga gcctttgacc cgcggcgtat
     6781 cgacggcgac acgctgcgat ctggcaagcg cgtcttgtcg cgagaacggc tgagaccgtt
     6841 gcgggcttgg aatcgtcgcg gcctgcggtg cgatggcttg cggttgcggt cggatggctg
     6901 cgtcgggcgc catcatgccg atctgcgaag tcttttgcgc gctcggtctg ccgaggaagg
     6961 acgtcgcgaa gaaatactgc caggtgatca ggatgaccag ggagagcgcg acagcataga
     7021 agacattgtt ctggtcgtgc atggtaagtc cccgttgcta tcaggcgcgt tccgtccttt
     7081 cgacggaagt cgtgcgccct taccggatca ttctagcata ttggtgacga ggcaagccca
     7141 tgcgcgactg attgtgatcc gcttgaattg tgcgctaccc gccctccagc gccaacgggg
     7201 caatcgcctc tgaccgcgag agcgattcgg agcagggcgg cgatccgccc acttgcacat
     7261 cgcgttgtcg ccgcgaggcc attcggcacg aggccgattt acatttgatt catcgtaaat
     7321 gttaaaatga aattcgaatt cttcatccat gatcgccgcg ttcggccatg gggccaggcg
     7381 cttgttgctg cgcgaagttg gtgttgcgat gagcgctgca cgacagacgt ggccgacttg
     7441 cctcgttttg aattgggaaa catgaacatg gcgatcgcgg gtcgagcgcc ctctggtgac
     7501 agtgcatcgc gtggcgtgcg atcgggcacc ggaggttttg gcgcactgtc cgcgcatgct
     7561 gcaaagcttt tggccctcgc cgccctgtcg ctaacgatgg cgcttgccgg ttgcgctcaa
     7621 aattcggctc agcacgaagt caaaaccgac cccattgagg cggctgcacc tccccaggga
     7681 caccctgact ttcgaattcg ccggcccaac cgggcgctgc tgctatcgca accagccccc
     7741 gactgcgagt tcaggtcaac cgagctcaag acggtggatc cggatcaatg ggggcgcctg
     7801 agactagatt acgagcggca atgctaccaa caggcggaga agaaggtccg cgaccgtttg
     7861 cggctgctgc aggcgtccag cagatgcgag attgagcccg ttcggacccc gctggcgacc
     7921 atccggtaac cggttccgcc gcgacaggcc gagtccatca acggccggac cagatggtca
     7981 ttcgaccttt caccgttgcg gtgcggtcat accgatctca cacggatcca ggcccactcg
     8041 aaacgaccga tacctcccgc taatccccgt catagacagc ggcggtattg cgctgcgatg
     8101 aacgagtcat gttttggcca tactgaaaca tcagtttttg aatacccatg gccttgttag
     8161 tcggcgactc cacggtcgga atttctgagg gggagcgtcc gcgagagtaa ccagtcaacg
     8221 atgaagactc tctacgattt gctcggcgcg cttccggacg acgacgccga aagcctcagg
     8281 acggcattcc gccaggccgc caaagcgacc caccctgaca ccaatactga cgatccggat
     8341 gctccgctaa aattcaggca gattgtccgt gccaatgcca tcctgagcga tgcggatcag
     8401 cgcgcgacat acgaccggct gctggcgctc acactccggc agcgccattg gaagttgaag
     8461 cgtaccatcc gcaaggtcgc ttccgatgcg atcaaggttg cttgcctttc ggccgtgtcg
     8521 atcggaggat acttggtttt cgagcaggtc tcgaaggcat ccatcgttcc ggtaaaaccg
     8581 accgaaattg cggcgcgcgc acggaccgag atcgccgctg tcacgccaac gaaacaatcc
     8641 gatacaaccg gccgcgataa gccaccggaa aagctggaga gtgcaggggt cgccagcgac
     8701 gcaatcgtgc cgagcggcgc tgcacccgcg acgaatgctg cacccgcggc gaatacagac
     8761 agcactcagg caatggccaa tgccgacccg gcgcccgacc tcacagcgcg cgatgccaaa
     8821 tcctaccgag agcggggaat cgctgcgtat atcggtggcg acctgtaccg cgcgctcgcc
     8881 gacttcgacg tggcgattca gatcgatcct ggctttgcgg atgcctacat cgatcgcggc
     8941 atcgttttct atcgtatcca cgaattcgac cgcgcgttcg ccgatattgc tcaggcgaag
     9001 cgtgtcaaga actcgagccg gaccaagact cttccgttgg cgccgcgcaa ggtatcgtca
     9061 tcatccgcca agaattgaac ctggtggaat gcctgtgctt gcctgtccgg cacgccgcct
     9121 tcgattgaaa acagtcactc gctcgcaagc gcgattgtat gcacatgccg gccgtagtcc
     9181 ggctcgccgc gatgcaccga gcggcgatag ctgaagaagc gctcgtcgga ataggtatcg
     9241 atgccggtgt cgtcgatcat caggacgccg gcgttttcga gccgcatccg gatgaagccg
     9301 gcaaggtcga acatcgaatg gccctcgcgc gccgcgggaa tgaaaaacaa ggtattctcc
     9361 gcatccgcct gcacgaagcg ttcgacgaat tcgctgccga cttcgtagga gtgctggcgg
     9421 atcagcgggc cgatcgcggc gacgatgccg ctgcgttcgg cgccgagctg ttccatcgcc
     9481 tctacggtgg gttccacaat gcctgtcagc gcgcctttcc atccggcatg cgcggctcca
     9541 atcacgcgcg cgctcggatc gaccagcagg atcggtccgc agtcggccgc ggtggcgccg
     9601 atggcgagac cttcgttgcg tgtgacgatg gcgtcggcca gcggccgcga cacgccttgc
     9661 cagggtccgc tggccaccac gacattgggc gaatgggtct ggtgcacgct gagaaagtgc
     9721 tcggggatga cgccgatctg ctccgccatc cggcggcggt tctccgcgac agaagcggga
     9781 tcgtcgcggg agccgaggcc gccattgagg ctggcgtaga tgccaccgga caccccgccc
     9841 tcgcgcgtaa agaacgtgtg acgcaggccg ggaatggcag agagcagggg cgatcccaac
     9901 ctcatggcag ttcggccccg acatcttgct gatcctgatc gttcagcccc gccagcgagg
     9961 caaggccggg ttccgagatc gcgagcacct tgaacatcga ccccatgccg ccacggccgt
    10021 cgccggtcag ccgtttcagc gcgctcgaaa tgtcctcgga aatctcatgc gtggtcttcg
    10081 ccatcagcgt aagcgcgcgg gtttcgatgc cgagccgttt cagaaactca ccctgtggca
    10141 ccgggccatg ggcacgcgcg ccgagatcct cgacggcgcg caccagggcc tgaaaatcga
    10201 catgggcggt gacgtcggcc agaccggggt ttttcagggg gtcggcgaag ctgtggcggg
    10261 caatggcctg aaaggtatcg ccggcgtcgc tgcgaacgtg accgtagtca atgatcagcg
    10321 ccgcgccctg ctgatcgcgg acgcgggtcg cgatcttcat gatctcggcg tcgggacgcc
    10381 attcgaacac cgcgccgacc ggcgcggcgc gcaccagtgg cggcagcagg acttcaaagc
    10441 gcggggttgg ttcggcagcg aaaccgaacg ccagcttgcc gttgccgtcg agctcgacgg
    10501 tacgctcgtg ccagccggtc tcgcgtttca ccatctggtg gatcggcagg acatcgaaat
    10561 attcgttggc gaaaatgacc gcgggaccct tgggcacctc gtcgatgctg ttgtgccagg
    10621 caatgacacg cacgcccgac agcgcagcct tctgcttttc gcgcagcacc ggattgatct
    10681 cgacgagatg gatgctcagc gcctgataga gcggcggcag cacccgcagc gcgcgcagcg
    10741 catccgccat catggtgccg cggccgggtc ccagttcgat cagtcgcagc agcgatggcg
    10801 aaccgatcgc cttccatacc gacgccgccc acaacccgag caattcgccg aacatctggc
    10861 tcacttcggg cgaggtgatg aaatcgcctt cgcgccccag cggatcgcgc gaaacgtaat
    10921 agccgtgttg gggatgcgac aggcacagct ccatgtaacg ccagaccggc atcggcccgg
    10981 acgacttgat gagctgcttg atttcggact ggagcggcga atactcgctc acggcttgcc
    11041 ctcgatcggg ttcggcatct ggttcgacat ggttcggcgc catgccacca caataatgat
    11101 cgcgcctgca atgatcatcg gcaccgacag cagcatgccc atggtcagcc cgccccacaa
    11161 gaatc
//
LOCUS       DASWGM010000011         3989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2357172,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000011 DASWGM010000000
VERSION     DASWGM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357172"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1465
                     /locus_tag="VGA15_00320"
     CDS             23..1465
                     /locus_tag="VGA15_00320"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX9586174.1"
                     /translation="MTHATGSPVVDNTNIQTAGPRGPALLQDIWLIEKLAHFDREVIP
                     ERRMHAKGWGAHGTFTVTKDITRYTKAKIFSELGKQTPLFIRISTVAGERGAADAERD
                     IRGFALKFYTEEGNWDIVGNNTPVFFFRDPLRFSDLNHAIKRDPRTGLRSADNNWDFW
                     TLLPEALHQVTIVMSDRGIPRSFRHMHGFGSHTFSMINAANERVWVKFHFRTQQGIQN
                     LTDEDAAAVVANDRESHGRDLLKAIESGNFPRWTLSIQVMTEAQARTHKHNPFDLTKV
                     WPKADYPLIEVGVMELNRYPDNYFAEVEQAAFSPANIVPGIGFSPDKMLQGRLFSYGD
                     TQRYRLGVNFNHIPVNAPKCPFHSYHRDGAMRTDGNLGATPTYWPNSKGSWMDRNEAL
                     IEPPLPIEGDATYWDHRIDDDHYEQPGILFRKMSPQQQRLLFENTARAMGDARIEVKE
                     RHIANCAKADPAYGAGVAAALGITLDRAAE"
     gene            1566..2273
                     /locus_tag="VGA15_00325"
     CDS             1566..2273
                     /locus_tag="VGA15_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012758536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX9586175.1"
                     /translation="MTVIEGIGIPDSKLCKEITGLVRDTESELLFNHSSRVYYFGALT
                     GLRRGLQFDRELLYAGAMFHDMGLTRQHSSQTERFEVDGANTARDFLKAHNIAQSNID
                     LVWNAIALHTTPGIPVHMHPVIALVTAGVEMDVLGIAYEEFPDAQREAVVATYPRTPH
                     FKEDIIQTFYDWIKHKPNTTFGNVKADVLADKDPHFHRGNFCSVIRGSRWKSWARHLS
                     IRSTGGEQRQNSAGDGR"
     gene            2385..2972
                     /locus_tag="VGA15_00330"
     CDS             2385..2972
                     /locus_tag="VGA15_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9586176.1"
                     /translation="MQTVLESRLEDRQSIADLMTGWIHRDLAEWDKLRELFHPDGTIE
                     ITWFEGLASDFVDGSMRMGESDFRTKHFISAPVVTFNGNKAIVETNAVVIGENVRLNL
                     GCSAHARFYDRVEKRNGVWKILKRQSIYDFGYFNFPQGVVEIDRELVKKYPREYAPLA
                     YLLEKSGFPVKRVFATKGSDLEKTMKANGQAWLAA"
     gene            complement(3181..>3989)
                     /locus_tag="VGA15_00335"
     CDS             complement(3181..>3989)
                     /locus_tag="VGA15_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9586177.1"
                     /translation="RFGSRVSIVEVGPQLAGREDPDVAAAVLEMLHDDGIEVHLGIKV
                     LRVQGRSGQTVSLQMRTPAGDRTVEGSDLLVAAGRTPNTDGIGLDLAGVVLDDRGYIA
                     VNDRLETSAPDVWAIGEAAGSPQFTHVSFDDFRIISANLSGGNRTKRDRLVPYCMFTD
                     PPLARVGLSEGEAQRQGIAVRVAKLPIVAVLRSRTISETRGFMKALVEAGGDRILGFA
                     MIGAEAGEVVAVVQAAMLADMPYMGLRDAIIAHPTMAEGLGALFSNVRAR"
BASE COUNT          812 a         1288 c         1211 g          678 t
ORIGIN      
        1 ggtgaccgac cgcaccccgc ccatgaccca tgccaccggc tcgcccgtgg tggacaacac
       61 gaacatccag acggcgggcc cgcgcggccc ggcgctgctc caggacatct ggctgatcga
      121 gaagctggcg catttcgacc gggaggtgat ccccgagcgg cgcatgcatg cgaagggctg
      181 gggcgcccac ggcaccttca ccgtcaccaa ggacattacc cgctacacca aggcgaagat
      241 cttctccgag ctcggcaagc agacgccact gttcatccgc atatcgacag tggcgggcga
      301 gcggggcgcg gccgatgccg agcgcgacat ccgaggcttc gccctaaagt tctataccga
      361 ggaaggcaat tgggacatcg tcggcaacaa cacgccggtc ttcttcttcc gcgatccgct
      421 gcgcttctcc gacctcaacc acgcgatcaa gcgcgatccg cgcaccggcc tcaggagcgc
      481 cgacaacaat tgggacttct ggacgctgct gcccgaggcg ctgcaccagg tcaccatcgt
      541 catgtccgat cgtggcatcc cgcgaagctt ccgccacatg catggcttcg gcagccacac
      601 cttctcgatg atcaacgccg ccaatgagcg cgtctgggtc aaattccact tccgcacgca
      661 gcaaggcatc cagaacctca cagacgaaga cgccgccgcg gtcgtcgcca acgatcgcga
      721 gagccatggg cgcgatctgc tcaaggcgat cgagagcggc aattttccga gatggacgct
      781 gtcgatccag gtgatgaccg aggcgcaggc ccggacccac aagcataatc cgttcgacct
      841 gaccaaggta tggccgaagg ccgactatcc gctgatcgag gtcggcgtga tggagctcaa
      901 tcgctatccg gacaattatt tcgccgaggt cgagcaagcg gcgttctcgc cggccaacat
      961 cgtgccgggc atcggcttct cgcccgacaa gatgctgcag gggcggcttt tctcctatgg
     1021 cgacacccag cgctatcggc tgggggtcaa tttcaatcac atcccggtga acgcgccgaa
     1081 atgcccgttc cacagctatc accgcgacgg cgcgatgcgg accgacggca acctcggcgc
     1141 gacaccgacc tactggccca acagcaaggg ctcgtggatg gaccggaacg aggcgctgat
     1201 cgagccgccg ctgccgatcg agggtgatgc gacctattgg gaccatcgca tcgacgacga
     1261 ccactatgag cagcccggca tcctgttcag gaagatgagt ccccaacaac agcggctttt
     1321 gttcgagaac accgcccgcg ccatgggtga cgcgcgcatc gaagtcaaag agcgccatat
     1381 cgccaactgc gccaaggccg atcccgccta tggcgcgggt gtcgccgccg cgcttggcat
     1441 cacgctcgac cgggcagccg aatgactggc ttcgctcgcc tgaccgctct gccccctcgc
     1501 gccggcgaaa tggcgcaacg caggcacaac gcgacgtgaa acagccccaa tagaaggtag
     1561 acctcatgac cgtgatcgaa gggatcggca tccccgacag caagctgtgc aaggagatca
     1621 ctgggcttgt ccgtgacact gaatcggaac tgctcttcaa ccattcgagt cgggtttatt
     1681 attttggagc gctgaccggc ctgcgacgcg gcctgcagtt cgaccgcgag ctgctctacg
     1741 ccggcgctat gttccacgac atgggcctga cacgtcagca cagcagccaa accgagcgct
     1801 tcgaggtcga cggcgcgaac accgcgcgcg acttcctgaa agcccacaac atcgcgcaaa
     1861 gcaacataga cctcgtctgg aacgcgatcg cgctgcacac gacgccgggt atccccgtgc
     1921 acatgcaccc ggtcatcgcc ctcgtcaccg ctggcgtcga gatggacgtg ctcggtatcg
     1981 cttacgagga attcccggat gcgcagcgcg aggccgtggt cgcgacctat ccccgcacgc
     2041 cgcatttcaa ggaggacatc atccagacct tctacgattg gatcaagcac aagccgaaca
     2101 cgacgttcgg caacgtcaag gccgacgtcc tcgccgacaa ggacccgcac ttccatcgcg
     2161 gcaatttctg cagcgtgatc cggggatcac gctggaagag ctgggcgagg catctgtcga
     2221 tccgcagcac aggcggagaa caacgtcaaa actccgctgg cgacggacgc tagccgcact
     2281 gtcgccgatg tcgtgcctgg cgtaagccgg cgcgacgcgg tggcgcaatg tgccgatcac
     2341 cgacctcgtg ggggcctggc gacaacgagg tgcagaggta agaaatgcag acggttttgg
     2401 aaagtcgttt ggaagatcgc cagagcattg ccgacctgat gactggttgg atccatcggg
     2461 atctggcgga gtgggataag ctgcgcgagc tgtttcatcc ggacggcacc atcgaaataa
     2521 catggttcga gggtctggct agcgattttg tcgatggctc gatgcgaatg ggcgaatcgg
     2581 atttccgtac caagcacttc attagcgctc ccgtagtgac cttcaatggc aacaaggcca
     2641 tcgtcgagac taatgccgtt gtcattggcg agaacgtgcg cctcaatctg ggctgcagtg
     2701 cacatgcccg tttctatgat cgggtcgaga agcgcaacgg cgtatggaag atcctgaagc
     2761 gccaaagtat ctacgacttt ggctacttca acttccctca gggcgtggtt gagatcgacc
     2821 gcgagcttgt caagaagtat ccgcgcgaat acgcgccgct cgcctatctc ttggaaaaga
     2881 gtggttttcc cgtgaagcgc gtctttgcca ccaaaggaag cgatctggaa aagacgatga
     2941 aggccaatgg ccaggcatgg ctggcggctt gatgcggttt caccgggcag cgtgctcgcc
     3001 caatcccaac gtaggagaaa aataaatgag aaagacggtg atacagcatg ttctttcccg
     3061 tctgagcgca acctcgttgc agcgaaatcc gtgcccgctc ggcgtgagcc tctcctgcgc
     3121 ggcatgcggg aacggagtgc ggcggcgggt tcgccgccgc accaacaacc tgtgcaagcc
     3181 ttatcgggcc cgcacattcg agaagagcgc gccgaggccc tccgccatcg tcgggtgcgc
     3241 aataatcgca tcgcgcaagc ccatataagg catgtccgcc aacatcgcgg cctgaacgac
     3301 ggccacaacc tcaccggcct cagcgccgat catggcgaat ccgaggatgc ggtcgccgcc
     3361 cgcctcgacc agcgccttca tgaagccgcg cgtctccgat atcgtccgcg atcgtaacac
     3421 ggcgacgatc ggcagcttcg cgacgcgaac ggcaatgccc tggcgctggg cctcgccctc
     3481 gctcaagccg acgcgcgcga gcggcgggtc ggtgaacatg cagtacggga cgagccggtc
     3541 gcgcttggtg cgattcccgc cgctcagatt ggccgagatg atgcggaaat cgtcgaacga
     3601 gacgtgggtg aactgtgggc tgccggcggc ctcgccgatc gcccagacat cgggcgcgct
     3661 cgtctcgagc cggtcattga ccgcgatata tccgcggtcg tcgagcacga caccggcgag
     3721 gtcgagcccg atgccgtccg tgttgggggt gcgtccggcc gcgaccagga gatcgctgcc
     3781 ttcgacggtc cgatcgcctg ccggcgtgcg catttggagg ctgacggtct gtccggaccg
     3841 gccctgcaca cggaggacct tgattccaag atgcacctct atgccgtcgt cgtgcagcat
     3901 ctccagaact gcagccgcga cgtcgggatc ttcccggccg gcgagctgtg gtccgacctc
     3961 cacgatactg acgcggctgc cgaagcggc
//
LOCUS       DASWGM010000012         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_278571,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000012 DASWGM010000000
VERSION     DASWGM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278571"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGA15_00340"
     CDS             <1..361
                     /locus_tag="VGA15_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9586178.1"
                     /translation="AVTLISAILVLGGALAAGHRHRVYDAVILKTLGATRARLLGAYA
                     LEYLMIGFATAIFGVIAGSVAAWLIVTRLMTLSFIWQAGSAAGVVAAALVVTVGLGLA
                     GTLLALNQKPATVLRNL"
     gene            618..1409
                     /locus_tag="VGA15_00345"
     CDS             618..1409
                     /locus_tag="VGA15_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX9586179.1"
                     /translation="MSDLDRNYASPFGRAAGRVDAATVDAGLRAYMLRIYNYMSIGLA
                     ITGLAALGVYMAAVTADPAGDAVRVGKIYLTPFGYAMFVSPLKWLFMLAPLAMVFVIS
                     AGIDRLKPATAQMLFWVFSALMGISLSSIFLVYTHTSIVRVFFITAATFGALSLYGYT
                     TKRDMSGMGSFLMMGLFGIIIASLVNLFLASSPLQFFISVIGVLVFAGLTAYDTQRLK
                     NDYIYGYASQGGAVAERAAITGALSLYLNFINLFTLLLQLLGQRD"
     gene            1511..2035
                     /locus_tag="VGA15_00350"
     CDS             1511..2035
                     /locus_tag="VGA15_00350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9586180.1"
                     /translation="MTSPEIRAAAAADLPAITEIYEHAVRYGTATFELIPPDLAEMTR
                     RFGVLMDGGFPYLVAVLEGHVVGYAYAGAYRPRPAYRFTVENSVYLQPAIHRRGIGLQ
                     LLQRLITESERRGYRQMIAVIGDSANAGSISVHTKCGFQMIGTHPNVGFKFGRWLDTV
                     MMQRALGDGGSTLP"
     gene            complement(2137..2508)
                     /locus_tag="VGA15_00355"
     CDS             complement(2137..2508)
                     /locus_tag="VGA15_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX9586181.1"
                     /translation="MGSISEEADPSESRAAARNAAAGPLPSRVTFNRFELNRILNLYG
                     RMVADGEWRDYAIDFLRDRAVFSVFRRASEVPIYRIEKDPRLARKQGIYSVISASGLI
                     LRRGHELDRVLLVIDRKLAVV"
     gene            complement(2978..>3495)
                     /locus_tag="VGA15_00360"
     CDS             complement(2978..>3495)
                     /locus_tag="VGA15_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX9586182.1"
                     /translation="ADSRFSARQKAYSRMRGDRDVYTPQAIVNGSVHVIGSDRAGIEG
                     AIGDTKKADGVMSVPVSLTLADKQISVSVGASGEIPTEAHGEVWICSISKTVPISIGR
                     GENGGREVTYYNVVRNLLKIGDWNGSSGSWTVPLESISREGVDAAVVYVQDGNRDKPG
                     PMLGAAYTSLH"
BASE COUNT          608 a         1126 c         1046 g          715 t
ORIGIN      
        1 cgcggtcacc ttgatctcgg ccatcctggt gctgggcggg gcgctcgccg ccggccatcg
       61 ccatcgggtc tacgacgcgg tcattctcaa gaccctgggc gccacccggg cgcggttgct
      121 cggcgcctac gcgctggaat acctgatgat cggcttcgcc acggcgattt tcggcgtgat
      181 cgcaggctcg gtggcggcct ggctgatcgt gacccggctg atgacgctga gcttcatctg
      241 gcaggccggc agcgccgcgg gggtggtggc ggcggcgctg gtggtcacgg tggggctggg
      301 gctcgccggc acccttttgg ccctgaacca gaagccggcc accgtgctgc ggaatttatg
      361 atagattgaa gcacccggcg gggctgacct ggccccgctt tccggtgctt ggccctgtga
      421 atcatgccgc ggagcgacat tcagccgcgc atggaagctt gcggtgaatg tgctagtttc
      481 ccacatatca gcttgggttc agacgccgat ttgatgacgt aaaattaatg tcagcaaatt
      541 tcggtatctg ggatacgttt cttaattggc ggcgcaaacc ccttgcgctc cgccggacaa
      601 acaacgggaa ttcgaccatg tcggacctag accgcaatta tgcttctcct ttcggccggg
      661 ccgccgggcg cgttgacgcc gcgaccgtcg acgctggtct gcgcgcctac atgctgcgca
      721 tctacaatta catgagcatt ggcctggcca tcaccggcct cgccgcgctc ggcgtctata
      781 tggcggcggt gacagccgac ccggcgggcg acgccgtccg ggtgggcaag atctatctga
      841 caccgttcgg ctacgcgatg ttcgtcagcc cgctgaaatg gctgttcatg ctggcgccgc
      901 tggcgatggt gttcgtgatc tcggccggca tcgatcggct caagcccgcg accgcccaaa
      961 tgctgttctg ggtattctcc gcgctgatgg gcatttcgct gtcgtcgatc ttcctggtgt
     1021 atacgcacac ctcgatcgtc cgggtgttct tcatcaccgc ggccacgttc ggcgcgctga
     1081 gcctctacgg ctacaccacc aaacgtgaca tgagcgggat ggggtcgttc ctgatgatgg
     1141 gcctgttcgg catcatcatc gcgagcctgg tcaacctgtt cctggctagt tcgccgctgc
     1201 agttctttat ctccgtgatc ggcgtgctgg tgtttgcggg cctcaccgcc tacgataccc
     1261 agcggctgaa gaacgattac atctacggct atgcctcgca gggcggtgcc gtcgccgagc
     1321 gggcggcgat caccggcgcg ctgtcgctgt atttgaactt catcaacctg ttcacgctgc
     1381 tgctgcagct gctcggccag cgcgactgaa cgctttcgtc gtagcgaaaa ttcaagcccc
     1441 ggccgcgagg ccggggcttt ccttttggcg gggcttttct tttggcgtgg acgaaaataa
     1501 tctccgcgcc atgacctctc ccgaaatcag ggccgccgcc gcggccgacc tccctgccat
     1561 taccgagatc tacgagcacg cggtccgcta cggcaccgcg acgttcgaac tgatcccgcc
     1621 ggatctcgcc gagatgacgc ggcgctttgg cgtattgatg gacggcggct ttccctatct
     1681 ggtcgcagtc ctagagggcc acgtggtggg atacgcctat gccggcgcgt accggccgcg
     1741 gccggcctac cgcttcacgg tggagaattc ggtctatctt cagcctgcga tccaccgccg
     1801 cgggatcggc ctgcaactgc tgcaacggct gatcaccgag tccgagcggc gcggctatcg
     1861 ccagatgatc gcggtgatcg gcgattccgc caacgccgga tcgatcagcg tgcacacgaa
     1921 gtgcggattt cagatgatcg ggacccatcc caatgtcggc ttcaaattcg gccgctggct
     1981 cgacaccgtg atgatgcagc gcgcgctcgg cgacggcggc tcgacgctgc cctagactct
     2041 gtagcccgga tggagcgcag cgaaatccgg gaacgaacgc gaagcctgcc ccggatttcg
     2101 cttcgctcca tccgggctac aggaccaaca ttctcgctac accaccgcca gcttgcggtc
     2161 gatcaccagc agcacgcgat cgagttcatg gccgcggcgc aggatcaatc cgctcgccga
     2221 gatcacgctg taaatgccct gcttgcgcgc cagccgcgga tctttttcga tgcgatagat
     2281 cggcacttcg gacgcgcggc ggaacaccga gaacaccgcg cgatcccgca agaaatcgat
     2341 ggcgtaatcg cgccattcgc catcggcgac catgcgcccg tacagattca aaatacgatt
     2401 gagttcgaaa cgattgaacg tcacccggct tggcaaagga ccagcagcgg cattgcgcgc
     2461 cgctgcgcgg ctctcgcttg gatcggcttc ctccgatatc gaacccatcg agcgcctcct
     2521 gtcatattac ctacatgatt gcgccgactg cctgccgccg caagggggct tccgacgacc
     2581 gggccgagat tattgcacgg cggattgttc gtgacccctt gcccggcaaa aaatgcagcg
     2641 tcacgggatc gttagctgaa atcataatac ggcccaactt ccgcccagcg accgcccttc
     2701 tggattgcga caaacaatca ctgcgttggc ccggtccttt cagctccgga ttcctcagcc
     2761 cccagccccc cggggcagtc gggatcgggc ctgttcgcag gatagtttat ccccaccctg
     2821 ggccagcgaa agttggtcct ttttcttttg gggggatgtt gtcattccgg ggcgcgctgg
     2881 cgctagcccg aaatctcctt ccaatacgtt cggaatgccg ggcctgcgcc aagcggcgca
     2941 tccccgaatg acggtgccaa taacgaagta atggaagtta gtgaagcgac gtgtaggccg
     3001 cgccgagcat cgggcctggt ttgtcgcgat tgccatcctg gacatagacc accgcggcat
     3061 cgacgccctc gcgggaaata ctttccagcg gcacggtcca gctgccggaa ctgccgttcc
     3121 agtcacctat cttgagcaga ttacgcacca cattgtagta agtgacttcg cggccgccgt
     3181 tctcgccgcg cccgattgaa attggcaccg tcttcgagat cgaacagatc catacttcgc
     3241 catgcgcttc ggtgggaatc tcgccggaag ccccgaccga aacggagatt tgcttgtcgg
     3301 ccagcgtcag cgaaaccggc accgacatga caccatcagc ctttttggta tcgccgatgg
     3361 cgccttcgat cccggcgcga tcacttccga tcacatgcac cgatccattg acgatggctt
     3421 gcggcgtgta gacgtcgcga tcgccgcgca tccgggaata ggccttctgg cgcgcgctga
     3481 agcgcgaatc cgcca
//
LOCUS       DASWGM010000013         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_971244,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000013 DASWGM010000000
VERSION     DASWGM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971244"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VGA15_00365"
     CDS             <1..703
                     /locus_tag="VGA15_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9586183.1"
                     /translation="NAEEPFLPLSLMGGTVAPYAMASGGFALGAILGLTVHLPLYYEV
                     VYHLSASEAGLALIPLAAVSTGGAAIAGRTMARAKHYKRVAIIGTGCAALAGCVLALT
                     ALPLWVLLVLLSVFALGLGTAFPISVVSLQNSVARSQVGTITGAMNFFRALMASFTVA
                     AFSAILLMALGADISLGGEHRGAVSSIPVADMITAFRYVFGAAAALLACASLCMILME
                     EKPLAGPATRLEMAE"
     gene            830..1051
                     /locus_tag="VGA15_00370"
     CDS             830..1051
                     /locus_tag="VGA15_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586184.1"
                     /translation="MSKPTIEQTRMGAEGIAFCIARTLIERDPSLKAPMRANLRKMWE
                     LLEEREDHAAADMVDTMIKALNDPAFFKP"
     gene            complement(1154..>1501)
                     /locus_tag="VGA15_00375"
     CDS             complement(1154..>1501)
                     /locus_tag="VGA15_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9586185.1"
                     /translation="EALFRQQFRRELRAQRFVMDFAISAADALARIASTIEQSLILIL
                     SDINMPGMTGLEMLPKVKEMRPDVPVIMITAYGDPETKRKAIESGATGLLTKPIDFSL
                     LREEIDTRLERAS"
BASE COUNT          251 a          452 c          491 g          307 t
ORIGIN      
        1 aaatgccgaa gagccgttcc tgccgctgtc gctgatgggc ggaacggttg cgccttacgc
       61 gatggcctcc ggcggcttcg cgctgggcgc gatactgggg ctgaccgtgc atctgccgct
      121 ctattacgag gtggtttatc acttaagtgc cagcgaggcg gggcttgcgc tgattccgct
      181 ggcggcggtt tcgaccgggg gggccgcgat tgccggccgc accatggcgc gcgccaaaca
      241 ctataagcgg gtcgcgatca tcggaacggg gtgtgcggcg ctggcgggat gcgtgctggc
      301 gctcacggcg ctgccgctat gggtgctgct ggtgctgttg tcggtgttcg cgctgggcct
      361 tggcacggca ttcccgatca gcgtggtctc gctgcagaat tcggtagcgc gctcgcaggt
      421 cggcaccatc accggcgcga tgaatttctt ccgcgcgctg atggcgtcct tcacggtggc
      481 ggcgttctcc gcgatcctcc tgatggcgct tggtgcggat atttcgctcg gtggcgagca
      541 ccgtggcgcg gtgagttcga ttcccgtcgc agacatgatc acggcattcc gctatgtgtt
      601 tggcgccgcc gcagcactgc tcgcctgcgc gtcgctgtgc atgatcctga tggaggaaaa
      661 gccgctggcc ggcccggcca cgcggctgga gatggcggag taaggcacca gacccctttc
      721 gtgttgtccg ccctgcggcc ttggctattc ataagccgcg atttgatcta acagcggcgc
      781 cagcaagtca gacaccgaaa ttcgagcgta attcgagaag gagccggcca tgagcaaacc
      841 gaccatcgag cagacccgga tgggcgccga aggcatcgcc ttctgcatcg cgcggaccct
      901 gatcgaacgc gacccctcgc tgaaggcgcc aatgcgcgcc aacctgcgca agatgtggga
      961 gcttttggag gagcgggagg accacgccgc cgccgacatg gtcgacacca tgatcaaggc
     1021 gctcaacgat cccgcgtttt tcaagccgtg aacttatttc ctcgcttggt gttctcgacc
     1081 gcgacgcgga cgatccggat gttcgagttg ggtcataacg ccgattggtc gagaacaatg
     1141 aggagccagc agttcaactt gcgcgttcca gccgggtgtc gatttcttcc cttagcaggc
     1201 tgaagtcgat cggcttggtc aacagtccgg tcgcgccgct ttcgatcgcc ttgcgcttgg
     1261 tttcgggatc gccataagcg gtgatcatga tgacgggaac gtcaggccgc atttccttga
     1321 ccttgggcag catttcaagg ccggtcatcc cgggcatgtt gatgtccgac aggatcaata
     1381 tcagcgactg ttcgatggtg ctggcgatgc gcgccagcgc gtccgcggcc gaaatcgcaa
     1441 agtccatcac gaaacgctgc gcgcgcaact cccgccggaa ttgctggcgg aacagcgcct
     1501 c
//
LOCUS       DASWGM010000014        13378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_278667,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000014 DASWGM010000000
VERSION     DASWGM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13378
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278667"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VGA15_00380"
     CDS             complement(<1..424)
                     /locus_tag="VGA15_00380"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9586186.1"
                     /translation="MTKLTAKAAGTFAIAPTPFLDDGRIDDKSIDRLTDFYAEVGCDG
                     VTVLGILGEAPKLDGSEAEEVAKRFVKRARNMQIIVGVSAPGFAAMRSLACASMDGGA
                     AGVMIAPPPHLRTDDQITGYFKQAIEAIGDDIPWVLQDY"
     gene            complement(556..1434)
                     /locus_tag="VGA15_00385"
     CDS             complement(556..1434)
                     /locus_tag="VGA15_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9586187.1"
                     /translation="MGLLDGKVALITGAGGGLGEAYAKLFAREGAAVVVNDLGGPRDG
                     SGGDKSMAQKVVDAILAEGGRAVANGADISTMAGGQSVFDDAIKHFGGADILVNNAGI
                     LLDQTFAKATEAAWDKVLKVHLKGTFCVTQPVFRWMRENGGGVIVNTSSTSGLIGNFG
                     QTNYGAAKGGIFGLTNVLAIEGRKYNIRLWCLAPGALTRMTADLPRYIENPNLALGPD
                     GIAPAVLYMVSDLSGDQTGKILGVSGPRGVREMRMMEKPGWKPPHVGWKAQDVVEHAK
                     EIFFSEEEIKMGARRF"
     gene            complement(1589..2449)
                     /locus_tag="VGA15_00390"
     CDS             complement(1589..2449)
                     /locus_tag="VGA15_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9586188.1"
                     /translation="MPIKYDELMALKNLGQKYAYTDREVMLYAYGIGMGADPMDEKEL
                     AFVNEATATPRALKVVPTFASVAAWGASPGEMNLNRVMVVDGERDITFHKPLAVAAHI
                     TADSSVLDVFDKGKDKGAVIRHKTVLKDEKGEPLATLVASRFARGDGGFGGPSEGQPE
                     PHKVPTRAPDRSIDISTRPDQALVYRLCGDRNPLHSDPEFAKRAGFAKPILHGMCTYG
                     ITCRGILQTYADYDASAFRQHVARFSSPVYPGETVTMDLWQDGNVISFEAKVKARNVA
                     VIKSGKTVLG"
     gene            complement(2660..3049)
                     /locus_tag="VGA15_00395"
     CDS             complement(2660..3049)
                     /locus_tag="VGA15_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEX9586189.1"
                     /translation="MDSKVKYPTPQGNPETRQFWDATVEGKLLIKRCTACGEPHYFPR
                     TLCPFCFSDKTVWEESSGEGEIYTFSLMRKSATGPYAIGYVTLKEGPSLLTNFVDCDM
                     DKLKIGQKVKVVFKTTDGAPLPFFTPV"
     gene            complement(3049..4197)
                     /locus_tag="VGA15_00400"
     CDS             complement(3049..4197)
                     /locus_tag="VGA15_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEX9586190.1"
                     /translation="MSIKGKAYLAGIYEHPTRHAPDKSTAQLHAEVAKGALEDAGLTK
                     ADIDGYFCAGDAAGGVMAMADYMGLKVRHFDSTDTGGCSYLVHLGHAAEAIAAGRCSI
                     ALITLAGKPRTGAMPPRATGAEVDFEAAYNATTHNIYGMCAMRHMHEFGTTSEQLAWI
                     KVAASHHAQYNEHAMLRDVVTVEDVLNSPMISDPLHRMDCCVVTDGGGALIVTTEAIA
                     RSLKRPLVRMIGAGEALKGPRGGKDLDLTYSAGVWSGPPAFAMAGVTQKDIKYASIYD
                     SFTITVLMQLEDLGFCKKGEGGKFVADGNLISGVGKLPFNTDGGGLCSNHPVNRGGMT
                     KILEAVRQLRGEAHPKVQVQNCDLALAHGTGGLLGVRHAASTCILERV"
     gene            complement(4801..5019)
                     /locus_tag="VGA15_00405"
     CDS             complement(4801..5019)
                     /locus_tag="VGA15_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586191.1"
                     /translation="MSGLAIQDIRVACACHERLADGQIGCELRRFARNNDLDAAGATK
                     QPDGQITKILSSPFRKNIPLSPSGKSMI"
     gene            complement(5118..6386)
                     /locus_tag="VGA15_00410"
     CDS             complement(5118..6386)
                     /locus_tag="VGA15_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586192.1"
                     /translation="MLHNGIVNVLGLEQGKDWVQVRRELTDEKISKIYRLYEALWPLE
                     TELFQLLPRPDAIARAIYTGSIHPTTITEFALGASLYFGELIIEHPFIHAGTVKKEFS
                     PVENPRTYRQEFLKTVLLFLTVMPLVDRGLVNLIPNICDFDVHLRDQMLHMARARSAG
                     TKIDSHTDTHLKELAKQDFRRSIMLLPRDALRSQLLKASPDLDKINLEEALRGIERLK
                     EHDPLAVLQEDSLAGGKKGGQFNVMKFAPNFEIAMYLAQATGSCIVTDTLFRWNEIKR
                     AIHQGVERSNTNLSALTHSIEGSVFAFPQNVEDIETLASGKTFACYPALMRDVFKYLS
                     KLDDRGPKPNVERHLAARFARAHLPAQAAIKKARIPIKEARISCVLPPGGIQDNTVNR
                     LLLMSNSERHLPSVPMAFLIKGHSQVRPTN"
     gene            complement(6386..7663)
                     /locus_tag="VGA15_00415"
     CDS             complement(6386..7663)
                     /locus_tag="VGA15_00415"
                     /inference="COORDINATES: protein motif:HMM:NF025388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HEX9586193.1"
                     /translation="MAGSSRTASRRERNLVHTRRKGVAQIGGSVAQMLAMSKTRNNHY
                     VPQWYQQGFFEPGRNTLAYLDLQPPQKVLDDGRVITERAIFDAPTSRCFRQMDLYSTF
                     FGTSVNDEIERRLFGDIDAKGSRAIRAFVGLDVGEWHRQFQTLFEYIDIQKIRTPKGL
                     DWLKTQYPRLTQNDLMIEMQGIRAMHCTIWTEGVREIISAEDADVKFIVSDHPVTIYN
                     HAVPPEVHNNAHPDEPGIALKASQTIFPLNRDLCLILTNLEYAKDPWTSPLDKRTFAR
                     NYRSSMVRTDAFIRTRKLSGQEVTRVNYVLKARARRYIAAGRKDWLYPEAVISDPWGE
                     LQKTLLPPMSGLWHFGGEMFVGYEDGHVHYQDEFGRAEKQREFLRKDPPVRPLRPGEL
                     CGCGFGIPLKNVANRSPLHCVQHGTSPVSANET"
     gene            complement(7943..11434)
                     /locus_tag="VGA15_00420"
     CDS             complement(7943..11434)
                     /locus_tag="VGA15_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX9586194.1"
                     /translation="MGINQGPISLDQKYTQGSGHVFLTGIQALVRLPMAQIRRDRAAG
                     LNTAGFVTGYRGSPLGGYDQQLFAARKHLEPYNIKFQPGVNEDLAATAIWGSQQLNLS
                     PGARYDGAVGIWYGKGPGVDRCGDVFRHGNAAGSAKNGGVLCLAGDDHGAKSSTVPHQ
                     SDHAFISALMPYLYPSSIHEMIEMGLLGIAMSRYSGCWVGMKVITETVETTAEIDLTD
                     EMTPFIIPTDFEMPPGGLNLRWPDDRYEQDRRLQDYKGFAAIAFARANKVNRVTMDSP
                     NARFGIMASGKSYEDIRQALRELGVTPEVAAKIGLRLYKIGMPWPLEPQGVREFAVGL
                     EEIFIVEERREIVENQVKQELFNWRDDVRPRIVGKMDEHDKRFLTFAAELSVASLASS
                     LTERLLRLNLNPEIAAMLRAKADWFNGREATQMQAVAPISRTPYFCSGCPHNTSTKVP
                     EGSRAFAGIGCHFMALWMDRSTETYTHMGGEGVPWVGVAPFTDEKHVFANLGDGTYFH
                     SGSLAIRQAVSSGANITYKILYNDATAMTGGQHVDGELSPQQITFQLHSEGIRDIYLV
                     SENPNAYPASDIAPGTRTAHRDELDAVMKTLREVKGASAIVFVQTCAAEKRRRRKRGT
                     LEDPARRVIINPAVCEGCGDCSVQSNCISVEPLETELGRKRTINQSSCNKDYSCLKGF
                     CPSFVTVDGGKLRRRAPAGLGEIGDLPEPASRPSLERPYNVAVGGVGGTGVLTIGALL
                     GMAAHIEGKASMILDMSGLAQKGGAVLSHVRLSEHTADVTCSRIVTGTADLVIAADEV
                     VAVAKDTISLCESSRTFGVINTHLIPIADFIMNRDFNFQSRKVNHVLETALRKDSSFF
                     DFTKPAEILLGDSIATNMMMMGYAYQKGLLPLSAKAIEQAIEINGVAIKMNTQAFQLG
                     RLAAVDPARIADMMKGQDAAIAPKTLDAMSLDEIIAHRSALLTDYQNKKLADRYRNMV
                     TQVRNAAANGGFGDELPRAVAINYAKLLAYKDEYEVARLYTDGRFEKQIRDQFDGDFK
                     ISFNLAPPMLGGGKDALGRPRKRAFGAWMLPVFRLLAGMRGLRGTAFDIFSRSADRRL
                     ERDLIAGYEKDVAHVLSVLSPLTLHTAIELLSLPDRIRGYGPVKEKAVSDAKARYAQL
                     AADLVNPPPAPRQIAAE"
     gene            11702..12907
                     /locus_tag="VGA15_00425"
     CDS             11702..12907
                     /locus_tag="VGA15_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9586195.1"
                     /translation="MRAIPAIILLCTAFNLGHAQAEPSAETIARGKALADAAGCASCH
                     TADPAKPFAGGKRIDTPFGGIYSPNLTPDRDTGLGAWSDDDFYRALRQGIAPDGSRYY
                     PAFPYPNFTKLIRDDILAIRAYLSTLAPIRNRRPQAELRWPLNYRVLMRGWDFLFFRP
                     GIFEPDQQKSPDWNRGGYLVEGAAHCGTCHTPKNLFGADKRGRAYGGGLVQGWFAPRL
                     DSAERGGLKSWSVDDIVEYLQSGRNGKSHASGSMAEVVVNSTSVMSDADVRAIAVYLK
                     DLPAGKREPAVSPPPPAQMADGAKLYAHACVACHETDGSGAPKIYPPLPGNANLQSAD
                     PSSTLRIILDGAQTLTTPRAPNTGSMPAYANQFSDQEIADVANYIRNSWGNAAPALTP
                     AQVAKARSR"
BASE COUNT         2524 a         4121 c         4016 g         2717 t
ORIGIN      
        1 gataatcctg cagcacccag ggaatatcgt caccgatcgc ttcaatggcc tgcttgaaat
       61 agcccgtgat ctgatcgtcg gtgcgcagat gcggcggcgg cgcgatcatc accccggccg
      121 cacccccgtc catcgaggcg cacgccagcg agcgcatcgc ggcgaatccc ggcgcggaga
      181 cgccgacgat gatctgcatg ttcctggcgc gcttgacaaa gcgttttgcg acttcctcgg
      241 cctccgaacc atcgagcttc ggcgcctcgc ccagaatgcc gagcacggtg acgccgtcgc
      301 agccgacctc ggcatagaag tcggtcagcc ggtcgatgga tttgtcgtcg atgcggccgt
      361 catcgaggaa cggcgtcggc gcaatggcga aggtgcctgc ggctttggcg gtgagttttg
      421 tcatgtgtgc ctctcgactt taattccgtc attccgggat gcgccgcttg gcgcaggccc
      481 ggaatccata ctcccggtcg tggttatgga ttccgggctc gcgctaagaa gcgcgccccg
      541 gaatgacaga tagcgtcaaa accgccgcgc gcccatcttg atctcttcct cggagaagaa
      601 aatctccttg gcgtgttcga cgacatcctg cgccttccac ccgacatgcg gcggcttcca
      661 gccgggcttt tccatcatcc gcatctcgcg cacgccgcgc gggccggaga cgccgaggat
      721 cttgccggtc tgatcgccgg acaaatcgct gaccatgtat agcacggccg gcgcgatgcc
      781 atcaggtccc agggccaaat ttggattctc gatatagcgc ggcaaatccg cggtcatgcg
      841 ggtcagcgcg cccggcgcga ggcaccacag ccggatattg tatttgcggc cctcgatcgc
      901 cagcacattg gtcaggccga aaatgccgcc tttggcggcg ccgtaattgg tctggccgaa
      961 attgccgatc aggcccgagg ttgaagacgt attgacgatg acgccgccgc cgttctcgcg
     1021 catccagcgg aacaccggct gggtgacgca gaaagtgccc ttcagatgca ccttcaagac
     1081 cttgtcccag gccgcctcgg tcgccttggc aaaggtctgg tcgagcagga tgccggcatt
     1141 gttgaccaga atgtcggcgc cgccgaaatg cttgatggcg tcgtcgaata ccgactgccc
     1201 gcccgccatg gtggagatgt cagcgccatt ggcgaccgcg cgaccgccct ctgccagaat
     1261 cgcatcgacg accttttgcg ccatcgattt gtcgccgccg ctgccatcgc gtggcccgcc
     1321 gaggtcgttg accacgacag cggccccctc gcgggcgaac agctttgcat aggcctcacc
     1381 gagcccgccg cccgcaccgg tgatcagcgc caccttgccg tcgagtaatc ccatgatgtt
     1441 ttgctcccgt gtcgtcgttt attcatccac cgtcatggcc gtgcttgtca cggccatcca
     1501 cgtctttctt ctccagagag aagacgtgga tgcccggcac aaggccgggc atgacgacat
     1561 cacttaaagc gcccgcccct gcataatctc accccagcac ggtcttgccg ctcttgatga
     1621 cggcgacgtt gcgcgccttc accttggcct cgaatgaaat cacgttgccg tcctgccaca
     1681 gatccatcgt cacggtttcg ccgggataga ccggcgagga gaaccgcgcg acgtgctgcc
     1741 tgaacgcgct cgcgtcgtag tcggcatagg tctgcaggat gccgcggcag gtgatgccat
     1801 aggtgcacat gccgtgcagg atcggttttg cgaagccggc gcgttttgca aactcgggat
     1861 cggaatgcag cggattgcga tcgccgcaga gccgatacac cagcgcctga tccggacgcg
     1921 tcgagatatc gattgatcgg tccggcgcgc gggtcggcac cttgtgcggt tcgggctggc
     1981 cttccgaagg cccgccaaag ccgccgtcgc cgcgcgcaaa acgcgacgcc accaacgtcg
     2041 ccaatggctc gcccttctcg tctttcagca cggtcttgtg gcggatcacc gcacccttgt
     2101 ccttgccctt gtcgaaaacg tcgagaacgc tggaatcggc ggtgatatgg gcggccacgg
     2161 ccagcggctt gtggaaggtg atgtcacgct cgccgtcgac caccatcacg cgattgagat
     2221 tcatctcgcc gggactggcg ccccatgccg cgaccgaggc aaatgtcggc acaaccttca
     2281 aggcgcgcgg cgtggctgtg gcctcgttga cgaacgccag ctccttctcg tccatcgggt
     2341 cggcgcccat cccgatgcca taggcgtaca gcatcacctc gcgatcggta taggcgtatt
     2401 tctggccgag atttttcagc gccatcagct cgtcgtactt gatcggcatt ttttgcgttc
     2461 ctccgaattt ttctttcgca gtgccagttg ttgcgccctc tccccttgtg ggagagggcc
     2521 acaccggcat cggcaacaaa cgctgcaggg tgaggggttg gcgcccgcgg atagatacct
     2581 ctccgctgat agagacccct catccggcag ccttcggctg ccaccttctc ccacaagggg
     2641 agaagggaag aaacatgcat cacaccggcg taaagaacgg cagcggcgcg ccgtcggtcg
     2701 tcttgaacac gaccttcact ttctggccga tcttcaattt gtccatgtcg cagtcgacga
     2761 aattggtcag cagcgagggg ccttccttca gcgtgacgta gccgatcgcg taaggcccgg
     2821 tggcggattt gcgcatcagg ctgaaggtat agatctcgcc ctcgcccgaa ctttcttccc
     2881 acaccgtctt gtcggaaaaa cagaacgggc acagcgtgcg cggaaagtaa tgcggctcgc
     2941 cgcaggcggt gcagcgtttg atgagcagct tgccctcgac cgtcgcatcc cagaactgtc
     3001 ttgtttccgg attgccctgc ggcgtcggat atttcacttt agagtccatc acacgcgctc
     3061 caggatgcag gtcgaggcgg cgtggcgaac gccgagcagg ccgccggtgc catgggccag
     3121 cgcgagatcg cagttttgca cctgcacctt cggatgcgcc tcgccgcgca gttggcgcac
     3181 cgcttcgaga atcttggtca tgccgccgcg gttgaccgga tgattgctgc acagaccgcc
     3241 gccgtcggta ttgaacggta gcttgccgac gccggaaatc agattgccgt cggcgacgaa
     3301 cttgccgccc tcgcctttct tgcagaaacc gaggtcctcg agctgcatca gcacggtgat
     3361 ggtgaagctg tcatagatcg aggcgtattt gatatccttc tgtgtcacgc cggccatggc
     3421 aaaggccggc ggtcccgacc acacgcccgc ggaataggtg agatcgagat ccttgccgcc
     3481 gcgcggtccc ttcagcgcct cgccggcgcc gatcatccgc accagcggcc gcttcaggct
     3541 gcgcgcgatt gcctccgtgg tgacgataag ggcgccgccg ccgtcggtga cgacgcagca
     3601 atccatccgg tgcagcggat cggagatcat cggcgagttc agcacgtcct cgacggtgac
     3661 gacgtcgcgc agcatcgcat gctcgttgta ttgcgcgtgg tgggatgccg cgaccttgat
     3721 ccaggccagt tgctcgctgg tggtgccaaa ctcatgcatg tggcgcatgg cgcacatgcc
     3781 gtaaatgttg tgcgtggtgg cgttgtaggc ggcctcgaaa tcgacctcgg cgccggtcgc
     3841 gcgcggcggc atggcgccgg tgcgcggctt tccagccagc gtgatcagcg cgatcgaaca
     3901 gcggccggcc gcgattgcct ccgcggcatg gccgagatgg accagatagg agcagccgcc
     3961 ggtatccgtg gaatcgaaat gacggacttt caggcccatg taatcggcca ttgccatcac
     4021 gccgcccgcc gcgtcgccgg cgcagaaata gccgtcaata tcggccttgg ttaaccccgc
     4081 gtcttccaac gcgcccttgg cgacttcggc atgcagctgt gcggtggatt tgtcgggtgc
     4141 gtgccgggtt ggatgctcgt agatcccggc gaggtaggcc ttacccttga tactcaagtc
     4201 gctctccgct gacatttctt cttgctagcg gtttttgtgg cctgtcggcg tggccttggc
     4261 aagcaccgaa atattccgtc aggcgagaca gtagccactg gaaccaagcc gggatgatta
     4321 tgccgaacat cgtatcaggc cggaacgcca acaccgtccg gcttcaagtt cgatgcccgg
     4381 agcggatatc acgaggcgtt ctcgcggcgc gacgcgcccg aggcttgcaa ggtaactttc
     4441 gcacctctcg aaaagagagg gcgcagggaa tgccggatgc gcgctgcacc cgcggtctcg
     4501 tgtgcatgtg cacaagaaga tgcgcacacg agcatacagg gcagtggagg caatccgaca
     4561 ttccctgcgc aatggcttta cggcttatag cgcgctctcc ccggtgatcg ggctttcttg
     4621 tcaccgtcgc cggcggaaac cgattccacc agcttgacgc cagcgtcgag gcgtcaggac
     4681 cacacgactt cgccgtacgt ttcagtgccg ttcgtcaaag gcgcatccac gtccaccgca
     4741 tcccaccgca cgttcgtgac gatcgcgagc cgcccctctt gtcgggtgag acggccggat
     4801 tcaaatcatt gatttgcccg acggcgaaag cggaatattt tttcgaaagg ggctggatag
     4861 aattttggtg atttgcccgt cgggttgttt tgtcgcaccc gccgcatcca gatcgttgtt
     4921 gcgcgcgaag cgacgcagct cgcagccgat ttgcccgtcg gccaatcgtt cgtggcaggc
     4981 gcaagcaacg cggatgtctt gaatggcaag accggacaac ggcaccgccg agattagccc
     5041 cttaaccgga cataggcgac ccaaaacaac cctcgcatac cacttactca aaccgctggc
     5101 ggctggttgt gcttcaccta gtttgtaggt cggacctgcg aatgcccttt aatcaagaac
     5161 gccatcggga cactcggtaa atgcctttcc gaattcgaca tcagaaggag acggttgacg
     5221 gtattatcct gaatgcctcc gggtgggaga acacaagaaa ttcgcgcttc cttgatagga
     5281 attcgggcct ttttgatagc agcctgagcc ggtaagtgcg ctcgggcgaa acgggcggcc
     5341 aaatgtctct ccacgttcgg cttgggcccg cgatcatcga gcttcgaaag gtatttgaag
     5401 acatcgcgca tcaatgctgg atagcaagcg aacgtcttgc ccgacgccaa ggtctcgatg
     5461 tcctccacat tttgggggaa cgcaaacacc gaaccctcga tgctatgcgt gagcgcggag
     5521 agattggtgt ttgacctttc caccccttga tggatggctc tcttaatttc gttccagcga
     5581 aagagggtat cggtcacaat gcaggaacct gtcgcttgtg ccaaatacat cgcgatctcg
     5641 aaattcggcg caaacttcat cacgttgaac tgcccgccct ttttgccgcc tgccagtgag
     5701 tcctcttgta gcacggccag agggtcgtgc tccttgagcc gttcaatacc ccttagagcc
     5761 tcctcaagat ttatcttgtc tagatctggc gaggctttca gtaattggga ccgcagtgca
     5821 tctcgcggca gcaacatgat gcttcgcctg aaatcctgct tcgcgagttc tttgaggtga
     5881 gtgtcggtgt gcgagtctat ttttgtcccg gctgatctag ccctcgccat atgcagcatc
     5941 tgatcgcgta gatggacatc gaagtcgcag atattgggga tcaggttaac cagaccgcgg
     6001 tcgaccaacg gcatgacggt gaggaataaa aggacggtct tgagaaattc ctgccggtag
     6061 gttctcgggt tttcgaccgg actgaactcc tttttgacag tccccgcatg tatgaagggg
     6121 tgctctatga tcaactcgcc gaagtaaaga gacgctccaa gcgcgaactc agtgatggtc
     6181 gtgggatgga tggagccggt atagattgct cgagcgattg cgtcgggtct tggcagcagc
     6241 tgaaacagct cggtctcaag cggccacaaa gcttcataca gacgatagat tttgctaatt
     6301 ttctcgtcag tcaactcgcg tcgaacctgc acccagtctt ttccctgttc taggccgagg
     6361 acattcacga tgccattgtg tagcatcagg tttcgttcgc ggatactggg ctcgttccat
     6421 gttggacgca gtgcaatggg cttcgattcg caacattctt taaagggatg ccgaatccgc
     6481 agccgcataa ttccccaggg cgtaacggcc tgaccggcgg gtctttcctt aagaattcgc
     6541 gctgcttctc ggcccggccg aactcgtcct gataatggac atgaccatcc tcgtacccca
     6601 caaacatctc gccgccgaag tgccagagtc cactcattgg tggcagcagg gtcttctgaa
     6661 gctcacccca aggatcggaa atgacggctt ctggatagag ccaatccttg cgaccggcgg
     6721 cgatataacg gcgcgcccgc gccttcagaa catagttgac ccgtgtgact tcttggcccg
     6781 aaagttttcg agtgcggatg aatgcgtcgg tgcgaaccat cgagctccgg taattgcgcg
     6841 cgaaagtccg cttatcgagc gggctggtcc aagggtcttt tgcatattcc agattggtaa
     6901 ggatcaggca aagatcccga tttaacggaa agattgtctg cgaagccttc aaagcgatcc
     6961 caggttcgtc cgggtgagcg ttgttatgca cctcgggcgg aacagcatga ttgtagattg
     7021 ttaccggatg gtcgctgaca ataaatttta catcagcatc ttcggcagag atgatttcgc
     7081 gtaccccttc cgtccagatc gtgcagtgca tcgcgcggat accctgcatt tcaatcatca
     7141 ggtcgttctg cgtcagtctg ggatattgag tctttagcca atccaatccc ttgggcgtgc
     7201 gaattttctg aatgtcgata tattcgaaga gcgtttgaaa ctggcgatgc cattcaccga
     7261 catcaagtcc tacaaacgcc ctaatggctc ttgaaccttt agcatcaatg tcgccaaaca
     7321 gacggcgctc aatctcgtcg ttgacggatg ttccgaagaa tgtcgagtac aggtccatct
     7381 gcctaaagca gcgggaggtg ggtgcgtcga agatggccct ttcggtaatc acgcggccat
     7441 catcgagaac cttctgaggg ggctgtaggt cgagatacgc aagcgtgttg cggccgggtt
     7501 cgaagaagcc ctgctgatac cactgcggaa cgtaatgatt atttcgagtc ttcgacatcg
     7561 ccaacatttg tgcaacgcta ccgccaattt gtgcaacacc ctttcggcgt gtgtgcacta
     7621 aattgcgctc cctgcgcgag gccgtgcgtg acgatccggc catggtgctc tttaagtaac
     7681 tcgtacttga tggtcggctt ccggcacata cccaagcatc gctccgatgc aacgatatgt
     7741 cggctgtcgg acgcagggcg gactcgtgca ggcaatctga tcggcgcact tatgggttca
     7801 cggccatccc ggcgaacgcc gggacccata accacagtcg ttggtggttt tagccaagct
     7861 ggagctccag ctttcacaaa aactgcattt ggtgggtatg gatcccggct caaggccggg
     7921 acgacgatgt tgtgagagtc gctcactccg ccgcgatctg ccgcggcgct ggcggcggat
     7981 tgacgagatc ggcggccagc tgcgcgtagc gcgccttggc atcgcttacc gccttttcct
     8041 tcaccggccc gtagccgcgg atgcgatccg gcagcgagag cagttcgatc gcggtgtgga
     8101 gcgtcagcgg cgacagcacg gagagcacgt gagcgacgtc cttctcgtag ccggcgatca
     8161 ggtcgcgctc gagcctgcgg tcggcgctgc gactgaagat gtcgaaagca gtgccgcgca
     8221 agccgcgcat gcctgccagc aaacgaaaca ccggcagcat ccaggcgccg aacgcccgct
     8281 tgcgcggccg tcccagcgcg tccttgccac cgcctaacat tggcggcgcg aggttaaaac
     8341 tgatcttgaa atcgccgtcg aactggtcgc ggatctgctt ttcgaaccgg ccgtcggtgt
     8401 aaagccgcgc cacctcgtac tcgtccttgt aggcgagcag cttggcatag ttgatcgcta
     8461 ccgccctcgg cagctcgtcg ccgaaaccgc cgtttgcagc ggcgttgcgt acctgcgtca
     8521 ccatgttgcg atagcggtcg gcgagcttct tgttctgata atcggtcagg agcgcgctgc
     8581 gatgcgcgat gatttcgtcg agcgacatcg catccagcgt cttcggcgcg atcgccgcat
     8641 cttgaccctt catcatgtcc gcgatccgcg ccggatcgac cgccgccaga cgcccgagct
     8701 ggaacgcctg ggtgttcatc ttgatcgcaa ccccgttgat ctcgatcgcc tgctcgatgg
     8761 ccttggccga gagcggcaaa agacccttct gataggcata gcccatcatc atcatgttgg
     8821 tggcgatgga atcgccgagc aggatttcgg caggtttggt gaaatcgaag aacgacgaat
     8881 ccttgcgcag cgcggtttcc aggacatggt tgaccttgcg gctctggaaa ttgaaatcac
     8941 ggttcatgat gaaatccgcg atcggaatga ggtgggtgtt gatgacccca aaggtgcggc
     9001 tggactcgca cagcgagatc gtgtctttcg cgaccgccac gacttcgtcg gcggcaatca
     9061 cgagatcggc ggtgccggtg acgatgcgcg aacaagtcac gtcggcggta tgctccgaca
     9121 gccggacgtg gctcaacacc gcgccgccct tttgcgcgag gccggacatg tccaggatca
     9181 tgctggcctt gccctcgata tgcgcggcca tgccgagcag cgcgccgatg gtcagcacgc
     9241 cggtgccgcc gacgccgccg acggcaacgt tgtagggccg ctccagcgag ggccttgatg
     9301 ccggttccgg cagatcgccg atctcgccga gaccggccgg cgcgcggcgg cgcagttttc
     9361 ccccatccac tgtgacgaag gacgggcaaa aacctttcag gcaggaataa tccttgttgc
     9421 agctcgactg gttgatggtg cgcttgcgcc cgagttcggt ctccagcggt tccaccgaga
     9481 tgcagttcga ttgcaccgag caatcgccgc agccctcgca caccgcgggg ttgatgatga
     9541 cgcggcgggc cggatcctcc agcgtgccgc gtttgcggcg gcggcgtttt tcggcggcgc
     9601 aggtctgcac gaatacgatg gcggaggcgc ccttcacttc gcgcagcgtt ttcatcaccg
     9661 cgtcgagttc atcgcgatgc gcggtcctgg tgccgggcgc gatgtcggat gccggatagg
     9721 cgttgggatt ttccgaaacc agatagatgt cgcgaatgcc ttccgagtgc agctggaagg
     9781 tgatctgctg cggcgaaagc tcgccgtcga cgtgctggcc gccggtcatc gcggtcgcgt
     9841 cgttgtagag gatcttgtag gtgatgttgg cgccggacga gacggcctga cggatcgcaa
     9901 ggcttcccga gtggaaatag gtgccgtcgc cgagattggc gaacacgtgc ttttcgtcgg
     9961 tgaagggggc aacaccgacc cacggcacgc cctcgcctcc catatgggtg taggtttcgg
    10021 tgctgcgatc catccacagc gccatgaagt ggcagccgat gccggcgaag gcgcggctgc
    10081 cctcgggtac cttggtcgag gtgttgtgcg ggcagcccga gcagaaatac ggcgtccggc
    10141 tgatcggcgc cacggcctgc atctgggtcg cctcacggcc gttgaaccag tcggccttgg
    10201 cacgcagcat cgccgcgatc tcgggattga ggttgaggcg aagcagccgc tcggtcaacg
    10261 agctcgcgag cgacgcaacg ctcagctcgg ccgcgaatgt caggaagcgc ttgtcgtgct
    10321 cgtccatctt gccgacgatg cgcggccgca cgtcgtcgcg ccagttgaac aattcctgct
    10381 tgacctgatt ctcgacgatc tcgcggcgtt cttcaacgat gaaaatctct tcgagcccaa
    10441 ccgcgaattc ccgcaccccc tgcggctcca gcggccacgg catgccgatc ttgtaaagtc
    10501 tgagcccgat cttggctgcg acttccggcg tcaccccgag ttcgcgcagc gcctgccgga
    10561 tatcctcata actcttgccc gaggccatga taccgaaccg cgcgttcggc gaatccatgg
    10621 tgacgcggtt gaccttgttg gcgcgggcaa aagcgatcgc cgcaaagccc ttgtagtcct
    10681 gcaggcgccg gtcctgttcg tagcggtcat cgggccagcg caggttcagt ccaccgggcg
    10741 gcatctcgaa atcagtgggg atgatgaacg gcgtcatctc atcggtgagg tcgatctccg
    10801 ccgtggtctc cacggtctcg gtgatcacct tcatgccgac ccagcagccg gaatagcgcg
    10861 acatcgcgat cccgagcagc cccatctcga tcatttcgtg gatgctggag ggatagaggt
    10921 agggcatcaa tgcggagatg aaggcgtggt ccgattgatg cggcacggtg gaagactttg
    10981 cgccgtgatc gtcaccggca aggcagagca cgccgccgtt tttcgccgag cccgcggcat
    11041 tgccgtgccg aaacacgtcg ccgcagcggt cgacgccggg gcccttgccg taccagatgc
    11101 cgaccgcgcc atcgtatctc gcaccgggcg acaggttcaa ctgctgcgag ccccagatcg
    11161 cggtggccgc caggtcttcg ttcacgccgg gctgaaattt gatgttgtac ggctcgagat
    11221 gttttcgcgc ggcgaacagt tgctggtcat agccgccgag cggcgaaccc cggtatcccg
    11281 tcacaaaacc cgcggtattg agaccggcgg cgcgatcgcg gcggatctga gccatcggca
    11341 ggcggaccag cgcctggatt ccggtcagga agacgtggcc cgacccttgg gtgtatttct
    11401 gatcgagact gatcggaccc tgattgattc ccattccatc ctctttgagc cgccgcgctg
    11461 aggctctttt acctagttat tttcagagct tagaaccagt ctatgtcgct ttccgcaagc
    11521 agcgcaccac aaatctcggc aatggagccc cgcgtccgca gtttgcaatt tcatgcccga
    11581 aacggcgccg ggacttttcg tcttgtgtcg ccgcatggcg cgacagcgaa acgacgtaac
    11641 gctcccgtcg acagggtttc gcgctataag acacaaatcg gtgcaggaca attattgcgc
    11701 aatgcgagcg attcccgcga tcatactttt gtgcaccgcg ttcaaccttg gtcatgcgca
    11761 ggccgagccc tcggcggaga ccatcgcgcg gggcaaggcg ctggcggacg ccgccggttg
    11821 cgccagttgc cacaccgccg atcccgccaa gccgtttgcc ggcggaaaaa ggatcgacac
    11881 gccgttcggc gggatctatt ccccgaacct gacgccggat cgcgataccg gcctcggcgc
    11941 atggagcgac gacgactttt accgcgcctt gcgtcaggga atagcgcccg acggctcgcg
    12001 ctattacccc gccttcccct accccaattt cacgaaattg atccgcgacg atatactggc
    12061 gatccgcgcc tacctctcta cgcttgcgcc gatccgcaac cggcggccgc aggcggaact
    12121 gcgctggccg ctcaattacc gcgtgctgat gcgcggctgg gattttctgt tcttccggcc
    12181 gggcatattc gagccggacc agcaaaaaag cccggactgg aaccgcggcg gctatctggt
    12241 cgaaggtgcc gcgcattgcg gcacgtgcca cacacccaag aacctgttcg gcgccgacaa
    12301 gcgtggccga gcctatggcg gcggcctcgt gcagggctgg tttgcgccgc gcctcgacag
    12361 cgccgagcgc ggcggactga aatcctggag cgtcgacgac atcgtcgaat atctgcagag
    12421 tggccgcaac ggaaaaagcc acgccagcgg gtcgatggcc gaagtcgtgg tcaattcgac
    12481 ctcggtcatg agcgatgccg atgtccgcgc catcgccgtc tatctgaagg atcttccggc
    12541 tggcaagcgg gagcctgcgg tgagcccgcc gccgcctgcg cagatggccg acggcgcgaa
    12601 actctatgcc catgcctgcg tcgcctgcca tgaaaccgat ggtagcggcg cgccaaaaat
    12661 ctatccaccg ttgcccggca acgccaatct gcagtccgcc gatccctcca gcacgctgcg
    12721 catcattctc gacggcgccc agacattgac gacgccgcgc gcgcccaata ccggctcgat
    12781 gccggcttac gccaaccaat tttccgacca ggagatcgcc gatgtcgcga actacatcag
    12841 gaattcatgg ggcaacgccg cgcccgcgct gacaccggcg caggtggcga aagcgcgatc
    12901 acgataaacc ggagaagcaa aatcgtcatg cccgggcaga agcgcgtctt cgcgctagat
    12961 gtcccgggca tcaacgtctt cgctgcgata aatgacaaag aagacgtgga tggccgggca
    13021 taggcgagcg gaagcgacgc cgtccttcgg acggctatag gcgagcggaa gcgacgccgt
    13081 ccttcggccg gctatgcccg gccgtgacgc gatgttggac cgggttcccc gtttggcagg
    13141 ttccgagagg cataatggcc gtctcttatc aggaaccgac gttgggccca tcggttgttg
    13201 tgggccaacg gaggaaccca tcatggccaa cgcacgcaat ccgaacgatc cctatcgttc
    13261 cccggacgat ccctatcgtt ccaatcttac cgacgacgac atccgtcgcc aggcccgcct
    13321 cgacagcgag ttgcaggctg atccagaact tgccgagggt cccgccaatg gcggcaga
//
LOCUS       DASWGM010000015         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_416670,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000015 DASWGM010000000
VERSION     DASWGM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416670"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VGA15_00430"
     CDS             complement(<1..489)
                     /locus_tag="VGA15_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9586196.1"
                     /translation="MADSEPTHDNPRNTRNLPAVVQEGEVLRPAADNWLTRAIRTLFG
                     WKPGSVRDDLQVVLDASTPDEVGFSAIERTMLRNILGLHERRIADVMIHRADIVAVKR
                     DIPLGELMSLFESAAHSRLVVYNETLDDPEGIVHIRDLLVFMTAKARAPQAGKATRKK
                     PFP"
     gene            complement(491..1012)
                     /gene="ybeY"
                     /locus_tag="VGA15_00435"
     CDS             complement(491..1012)
                     /gene="ybeY"
                     /locus_tag="VGA15_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX9586197.1"
                     /translation="MALSALPIAEVLVVADCWQTEPDAEAVIHRAIATAAEIVNAGPN
                     KAAGEAELAVMLTDDAGIRTLNNNWRGIDKPTNVLSFPALQPTGKSGPDDAPRMLGDI
                     AIAYETTRKEADDEQKPFDYHLSHLAVHGFLHLIGYDHENDDDAEAMETLEQEILAQL
                     GIPDPYADRERTG"
     gene            complement(1078..2133)
                     /locus_tag="VGA15_00440"
     CDS             complement(1078..2133)
                     /locus_tag="VGA15_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX9586198.1"
                     /translation="MPKSASDSPSLAPSRKLDRDMQIPPETQVVIDFDDNRAASALVG
                     PYGQNLALVERRLGVVVDSRGNHITIAGSRDGCDAARRVLETLYTQATQGHDLAQGDV
                     EGAIRAVIAQGSLFEFDAKNVRSAFETINLRKRPVRARTAAQDSYIRALKRHEMVFGI
                     GPAGTGKTWLAVAHAAQLFERKEVDRIILSRPAVEAGERLGFLPGDLREKVDPYLRPI
                     YDALYDLMDSRIVERALQAGEIEIAPLAFMRGRTLTNAVIILDEAQNTTSMQMKMFLT
                     RLGENSRMIITGDPSQVDLPNGQTSGLAEAAKLLDGVEGIAQVKFTAEDVIRHELVAR
                     IVAAYEGLPQRPATIKS"
BASE COUNT          354 a          657 c          713 g          430 t
ORIGIN      
        1 ggggaacggc ttcttgcgcg tcgccttgcc tgcttggggc gcgcgggcct tggcggtcat
       61 gaataccagg agatcgcgga tgtgaacgat gccttcggga tcgtcgaggg tttcgttgta
      121 gaccaccaga cgcgaatgcg cggcgctctc gaacaggctc atcaactcgc cgagcggaat
      181 gtcacgtttg accgcgacga tgtcggcgcg atgaatcatc acgtcggcga tgcgccgttc
      241 atgcaggccg agaatattgc gcagcatggt gcgctcgatg gccgaaaaac ccacctcgtc
      301 cggggtgctt gcatcgagca cgacctgcag atcgtcgcgg accgatccgg gtttccatcc
      361 gaacagcgtg cggatagcgc gggtcagcca gttgtcggcg gccggacgca gcacttcacc
      421 ttcctgcacc accgcgggca ggttgcgcgt gttgcgcgga ttgtcgtggg tcggctctga
      481 atccgccatc tcagcccgtc cgctcccgat ccgcataggg atcgggaatg ccgagttgcg
      541 ccaaaatctc ctgctcgagt gtttccatcg cctcggcgtc gtcgtcgttt tcgtgatcgt
      601 agccgatcag gtgcaggaag ccatgcaccg ccagatggct gaggtgataa tcgaacggct
      661 tttgttcgtc atcggcttcc tttcgcgtcg tttcataggc gatggcgatg tcgccgagca
      721 tgcgcggcgc atcgtcgggg ccgctcttgc cggtcggctg cagggccgga aacgacagca
      781 cgttggtcgg cttgtcgatg ccacgccaat tgttgttgag ggttcggatt ccggcgtcgt
      841 cggtcagcat gaccgcgagt tcggcttcgc cagccgcttt attcggtccg gcgttgacga
      901 tctcggccgc ggttgcgatg gcgcgatgga tgacggcttc ggcgtccggc tcggtctgcc
      961 agcaatcggc gacgacgaga acttcggcaa tgggaagggc agacagcgcc atcggtccgg
     1021 aatctctgtt gcgaggggaa ctcgccccca gcacgctcgc agcggcaggt ttgcgcatca
     1081 agacttgatg gttgccggcc gctgcggcaa tccttcatag gcggcaacga tacgcgccac
     1141 cagttcgtgg cggatcacgt cttcggccgt gaacttcacc tgggcgatgc cctcgacgcc
     1201 atcgagcagc tttgcggctt cggcgagacc cgaagtctgg ccgttcggca ggtcgacctg
     1261 ggaaggatcg ccggtgatga tcatgcggct gttctcgcca agccgcgtca ggaacatctt
     1321 catctgcatc gaagtggtgt tttgcgcttc gtccagaatg atgacggcgt tggtgagagt
     1381 ccggccgcgc atgaaggcaa gcggagcgat ctcgatttcg ccggcctgca gcgcgcgctc
     1441 gacgatccgc gaatccatca gatcgtacag cgcatcgtag atcggccgca gatagggatc
     1501 gactttctcg cgcaaatcgc cgggcaggaa gccgagccgt tcgccggcct cgaccgccgg
     1561 ccgcgacagg ataatgcgat cgacttcctt gcgttcgaac aattgcgcgg cgtgcgccac
     1621 cgcgagccaa gtcttgccgg taccggcggg accgatgccg aacaccattt cgtggcgctt
     1681 gagcgcgcgg atataggaat cctgcgcggc cgtccgcgcc cgcaccgggc gcttgcgcaa
     1741 attgatggtc tcgaacgccg atcgcacgtt cttggcgtcg aactcgaaca gcgagccctg
     1801 cgcgatcacc gcgcggatgg cgccttcgac atcgccctgc gccagatcgt gaccctgtgt
     1861 cgcctgggtg tacagcgtct ccagcacgcg gcgcgcggcg tcgcagccgt cgcgtgagcc
     1921 cgcgatggtg atgtgattgc cgcgcgaatc gaccacgacg ccgagccggc gttcgaccag
     1981 cgccaggttt tgtccgtagg gtccgaccag cgccgaggcc gcgcgattgt cgtcgaagtc
     2041 gatcacgacc tgtgtttcgg gcgggatttg catgtcgcgg tcaagtttgc ggctgggagc
     2101 gagcgaaggc gaatccgatg cgctttttgg caagggttca ggctcccgtt gtga
//
LOCUS       DASWGM010000016         2829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2218272,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000016 DASWGM010000000
VERSION     DASWGM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218272"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VGA15_00445"
     CDS             complement(<1..849)
                     /locus_tag="VGA15_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX9586199.1"
                     /translation="MALVPSPQWLDSGNNAWQLAAATFVGLQSIPGLTVLYGGIVKKK
                     WAINSAFMAFYAFASVLVVWILFDYNMAFGPQWFPFLGTPGLATSATFTTGQAIIPAA
                     ASGMPPLTFPMATLIFFQFVFAAITVIILAGSVLGRMNFTAWMIFCPVWMTLVYTVGA
                     FSIWGGGWLAAMGVADFSGGYVIHLAAGTSGFVAAWLVGPRLQADRDHFPPNSLLMTL
                     VGAGILWLGWNGFNGGDPYFANANAGAAVLNTNTCTAVALLVWTLMDKMAYGKPSVLG
                     AVNGMIA"
     gene            1062..1397
                     /locus_tag="VGA15_00450"
     CDS             1062..1397
                     /locus_tag="VGA15_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586200.1"
                     /translation="MRSMILPRLAAGICAWAVSVAVPAFSAEAPRGETAVTCTNVASG
                     TNWQIRIDYDKATVDANPARISDAEISWHEPADGGNYTLDRKSEKLTVVVASSTGGYF
                     LYHQCRLQN"
     gene            1402..>2829
                     /locus_tag="VGA15_00455"
     CDS             1402..>2829
                     /locus_tag="VGA15_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX9586201.1"
                     /translation="MVPEARRRHHRVTVQAAGRARARPAEIGAAAMAERISSVKVSVG
                     FASETGPRKRNEDFAGAVFGPELPEPRRDVVAAIADGIGSTKGGREAAEIAVRGFLDG
                     FCDLPETMEVRRAAATVLNALNGWIHSEGRRDTRLTGMGCTFTALVLRGRVAHVLHVG
                     DTRAYRLSRDRLTCLTTDHVRPGGAGRSNILTRALGVETEVRLDYTNQPMAQHDRFLL
                     CSDGVHAYLAAETIADIMRERVSSEDTARALVAAALQSDSSDNCTALVLDVVGLPTAE
                     SADIGANIAQLPIIPVPLGGETIDGFVLKVLLSDGRYSRLFGAEDEVEGGEVALKFPK
                     PQVASVATYRAAFVREAWVGARVTSPWVGHIIELPPGRQSCLYTVMPLYQGELLETRL
                     ARRPAVGLEEGRHIAIRLARAAAALHRAGIVHRDIKPDNVILEGEGSLKLIDLGVVRI
                     PGMEDFPPEEIPGTPGYMAPEMLDGE"
BASE COUNT          542 a          867 c          951 g          469 t
ORIGIN      
        1 cgcgatcatg ccgttgaccg cgccgagcac cgacggcttg ccataggcca tcttgtccat
       61 cagcgtccag accagaaggg ccaccgcggt gcaggtgttg gtgttgagca cggccgcgcc
      121 ggcattggcg ttggcgaaat acggatcgcc gccgttgaaa ccgttccaac ccagccacag
      181 gatcccggca ccaaccagcg tcatcaacag gctgttgggc gggaaatggt cgcggtcggc
      241 ctgcagccgc ggaccgacca gccatgcggc gacgaagccg gatgtgccgg cggcaaggtg
      301 gatgacatag ccgcccgaga agtcggccac gcccatggct gcgagccagc caccgcccca
      361 gatgctgaat gccccgaccg tatagaccag ggtcatccac accgggcaga agatcatcca
      421 cgccgtgaag ttcatgcgac cgagcacgga gccggcgagg atgatgacgg tgatggcggc
      481 gaacacgaac tggaagaaaa tcagtgtcgc catcggaaag gtcagcggcg gcatgcccga
      541 tgccgcagcc ggaatgatcg cttgaccggt cgtaaaggtg gccgatgtcg ccaagcccgg
      601 cgtaccgagg aatggaaacc attgcggccc gaacgccata ttgtagtcga acaggatcca
      661 gacgaccagg accgaagcga acgcgtagaa cgccatgaag gcggagttga tcgcccactt
      721 cttcttgacg atgccgccgt agagaactgt cagccccgga atgctttgca ggccgacgaa
      781 ggtcgcggca gcgagctgcc acgcgttatt gccggaatcc agccattgtg gtgatggtac
      841 gagtgccatg agcgtttccc cattttgatt ttcaagtcca atccgtgcgc cggtggcaca
      901 gaccagcgtt caagcaaaac gcaaggaata taccactcat tgccggctca actggacatt
      961 gatcgcgtac ggattttccg taaaagtcat gcggcgaaca gaggtcgatg cgatcaaaaa
     1021 atgtgcacat tgagtaaaaa ataagcaggg gaggcgtact ggtgcgatca atgattttgc
     1081 cccggctggc ggcgggaatt tgcgcgtggg cggttagcgt cgccgttccc gctttcagcg
     1141 ctgaagcgcc gcgcggcgag accgcggtta cctgcaccaa tgttgccagt ggcacgaatt
     1201 ggcagatcag gatcgactac gacaaagcta cggtcgatgc aaatccggcc cgcatcagcg
     1261 acgccgagat ttcgtggcac gagccggccg atggcgggaa ctacacgctt gaccgcaaat
     1321 ccgagaaact tacggtggtc gtggcgtcga gcaccggcgg ctatttcctt tatcatcagt
     1381 gccggctgca gaattgaggc cgtggtcccg gaagcgaggc ggcgccatca ccgcgtcacg
     1441 gtccaggcgg ccggccgcgc gcgcgcgcgc cccgcggaaa ttggagcggc ggcgatggcc
     1501 gagcggattt ccagcgtcaa ggtaagtgtc gggttcgcct cggagaccgg accgcgcaag
     1561 cgcaatgagg atttcgccgg cgccgttttc ggcccggaac tgcccgagcc gcgccgggac
     1621 gtggtcgctg cgatcgccga tggcattggc agcacaaagg gcggccgcga ggcggccgag
     1681 atcgccgtgc gcggtttcct cgatggattc tgcgatctgc ccgagacgat ggaagtgcga
     1741 cgcgcggcgg ccacggttct gaatgcgctc aatggctgga tccattccga aggtcgccgg
     1801 gatacaaggc tgacggggat gggatgtacc ttcacggcgc tggtgctgcg cgggcgcgtc
     1861 gcgcacgtgc tgcatgtcgg cgacacccgc gcctatcggc tgagccgcga ccgcctgacc
     1921 tgcctgacca ccgaccatgt acggccgggc ggcgccggcc ggtcgaacat ccttacccgc
     1981 gcgttgggtg tcgagaccga ggtgcggctg gactacacca accagccgat ggcgcagcac
     2041 gaccgctttc tgctctgcag cgatggcgtg cacgcctacc tcgcggcgga gaccatcgcc
     2101 gacatcatgc gcgagcgcgt ctcttccgag gataccgcgc gtgcgttggt ggcggccgcg
     2161 ctccaatccg acagcagcga caactgcacc gctttggtgc tcgacgtggt gggcctgccg
     2221 acggcggaat cggctgatat cggcgccaac atcgcccaac tgccgatcat cccggtgccc
     2281 ctcggcggcg agacgatcga tggctttgtg ctcaaagtgc tgctgtccga tggccgatac
     2341 agccgcctgt tcggtgcgga ggacgaggtc gagggcggcg aggtggcgct gaagtttcca
     2401 aagccgcagg tggccagcgt ggccacctac cgcgcggcct ttgtccgcga ggcgtgggtg
     2461 ggcgcgcgcg tgaccagtcc ctgggtcggc cacatcatcg agctgccgcc gggccggcag
     2521 agctgcctct acaccgtcat gccgctttat cagggcgaac ttctggagac ccggcttgcg
     2581 cgtcgccccg cggtgggact ggaggaggga cgtcatatcg cgatcaggtt ggcccgggcg
     2641 gcggcggcgc tgcaccgcgc cggcatcgtg catcgcgaca tcaagccaga caacgtgatt
     2701 ctcgaggggg agggatcgct gaagctgatc gatctcgggg tggtgcgcat accgggaatg
     2761 gaggattttc cgcccgagga gattccgggt acccccggct acatggcgcc cgaaatgctt
     2821 gacggcgaa
//
LOCUS       DASWGM010000017        21920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2357532,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000017 DASWGM010000000
VERSION     DASWGM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 21920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21920
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357532"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VGA15_00460"
     CDS             <1..813
                     /locus_tag="VGA15_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9586202.1"
                     /translation="FDYPLSSRLDENDAVFIMDKVLVPWENVFVYGDLEKANNFFPRT
                     GFLPRFVVHGCTRLAVKLDFIAGLLLKATEAAGTKDYRGVQANVGEVIAWRNLFWGLS
                     DAMVRDPKPWIGDYVLPNMDPGNAYQIIATMAYTRVKYLIEQTVASGLIYLNSHARDF
                     KNPEIRPYLDQYLRGSNGYKAEERVKLLKLLWDCLASEFGGRHELYEINYGGSTEEIR
                     RYCLFGAQASGNADKFKGFAEECMAEYDLDGWKVPDLSDPGELSYHVMRGAG"
     gene            982..1446
                     /locus_tag="VGA15_00465"
     CDS             982..1446
                     /locus_tag="VGA15_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9586203.1"
                     /translation="MPGSDRGRIIFRKLTDYQLGLYASSDYLRANEPIEDIRDLRQHH
                     FIGYIEDLLFAPELNYLPALTKNIAARIKSANLNAQLNTCLSGAGLAVLPSFMTRDLP
                     QLHRVLPEHTSLLRTFYMQMHEDSRQIGPVQETAKFILQQVRINRDLFNPDH"
     gene            complement(1845..2996)
                     /locus_tag="VGA15_00470"
     CDS             complement(1845..2996)
                     /locus_tag="VGA15_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9586204.1"
                     /translation="MTGPLDGFRVVEMGGIGPAPFAGALLGDLGADVLRIDRIARPGV
                     EPDLPPRFDFYNRNKRSVAFDLKQPQAVATVLKLMTEADALIEGFRPTVMERLGLGPE
                     VCLAVNPRLVYARMTGWGQKGPIAQEAGHDINYLALTGALHCLGDADRPPPPPLNLVA
                     DLGGGALYLTVGLLAAAMEARRSGRGQTIDVAMIDGVTHLMSAFQAFRQQGSWTERRA
                     DNIVDGGAPFYRSYATGDGKFVAVGAIEPHFYANLMKVIGIDGENLPDQNDRTAWPQM
                     RERFAKIFAMRTRDEWVAAAAGRDACLAPVLTIDEAPEHPQMQAREVYAMFDGLLHPS
                     PAPRFSRTPSKLRWPTPAPGRDSRQALADWGIPPDQVAALEAAGAMEQA"
     gene            complement(2993..3520)
                     /locus_tag="VGA15_00475"
     CDS             complement(2993..3520)
                     /locus_tag="VGA15_00475"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9586205.1"
                     /translation="MTSSDSTAEIAGPVGVVGTGLMGLGIAQAVASAGFAVVLCGRNA
                     EASHQGLNEGPRALWLGLADLYPSAAAQPDEGSVRLRLLAAQAREVLRCLEDGVIESA
                     DDADAASVFGLGFPKPLGGVARWVEDFGLGELVALCRCLAKAHSGRFASTSWLDGLAA
                     SGKGLSDFRKQEISS"
     gene            complement(3495..4181)
                     /locus_tag="VGA15_00480"
     CDS             complement(3495..4181)
                     /locus_tag="VGA15_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX9586206.1"
                     /translation="MSMHERPAMNVDDAIRHRRSVRGFLSDEVPEATLREVFELAQWS
                     PSNCNVQPWTPHVVSGDALKRLRGALVAAGMRDEPIKPDWPADGKFTGIYRERQVDAA
                     QKLYGAMGVERRDVVGRKMAYIRNHAFFDAPHAVLIFMPEPFDAREAADIGMYAQTLM
                     LALVARGIASCAQGALGLFPEIVREHLGIPANCKLLFGISFGYEDRSIKANAARVGRA
                     HIDDVVRFHR"
     gene            complement(4178..5230)
                     /locus_tag="VGA15_00485"
     CDS             complement(4178..5230)
                     /locus_tag="VGA15_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9586207.1"
                     /translation="MKTETESNGADRGIDFDVARLDEYLKGWLGGVHPTRVERTHGGM
                     SNPTYFVTRGEWRAVLRKQPNNVLMPSAHAIDREYRVLTALQGSAVPTPKPYSYCEDH
                     EIVGTPFYLMEWLDGRVFNDFATPGLTRGERAELFRSMCATLAAIHKLDYQALGLGDF
                     GKPGNYFARQLNRWSKQWTQFRRGDDDNPALDRMVHWLAERLPESETIALCHGDFRIA
                     NAMFHEKEPHVIGVLDWELSTLGHPLVDLAFNSQAWRMAPDENGGLLGLPLDEMGVPS
                     EHDYLELYYELSGAKERLTRFHQVFAMFRGAVGSAGVAARGDLGSGVLPDAARVGRHL
                     ARAYAKRGVALIEEQR"
     gene            complement(5227..6474)
                     /locus_tag="VGA15_00490"
     CDS             complement(5227..6474)
                     /locus_tag="VGA15_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9586208.1"
                     /translation="MDFELPREITDKLVELDAFIEAEIKPLERENMQFFDHRRENART
                     DWDDDGRPRTEWRALIGEMERRADKAGHLRYGLPKSCGGHAASNLAIAAIREHLAAKG
                     LGLHNDLQDESSIVGNFPIIPALADYGTKEQKTYIEGIITGKKHLSFGLTEPGHGSDA
                     TWLETTGVRDGDHWIINGRKRWNSQVARAHANLVFARTSGKPGEATGISAFIVPTDTP
                     GHNVLYNHWTFNMPSDHAETELTNVRVPDSAILHKEGEGLVVAQRFVHENRVRQAAAS
                     AGAARYCIQEAVKYAKSRVAFGEPLAKKQAIQFPLVELWAEYELVRNFIFRTAWQMDR
                     QNPLEISDMVSICNFRANRLCCEAADRAMQVHGGIGYSRALPFEHIYRHHRRYRITEG
                     TEEVQIRRVAQYLFNFSGKRASQ"
     gene            complement(6515..7300)
                     /locus_tag="VGA15_00495"
     CDS             complement(6515..7300)
                     /locus_tag="VGA15_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9586209.1"
                     /translation="MKRLEGKRAVVTGGGSGIGRASAIRFAREGASVLVVGRAGNTEE
                     TAATIRGEGGMASALIVDASIEENVAAMVGRCVSEFGGLEVFFANIGVTGTNTPLLEQ
                     TVEEWSEVYRVNTISCFLAIKYAGRHMTAQRHGSIILTSSAASLRANAGATSYSASKA
                     AVNNLAQTAANAFAGTGVRVNAVLPGLVETQLTRKVFEDARARGVEAKLGHVTPMKRP
                     GRSEEIAAAAAFLASDDSSYVDGQLLSVDGGVSSTHPYGRIAL"
     gene            complement(7311..8072)
                     /locus_tag="VGA15_00500"
     CDS             complement(7311..8072)
                     /locus_tag="VGA15_00500"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9586210.1"
                     /translation="MNSRLKDRVCVVTGAARGIGLAIAERFGREGGRVACVDVSARRL
                     EPAVNELRHKGCEARAYEVDVGKRDDVHALFARIESEFAAPVAVLVNNAVWARFEPLP
                     QIEIETVERMFAVGVHGLIWTTQAVAPQMERRGGGSIVNLSSVSAFHPAKDSIAYSAL
                     KAGVVGLSRAAAIELSAKKIRVNVIAPGMIATPASIAQFDEATLATRQAEMPSGRFGA
                     PEEIASLAAFLASDDASYIQGAVITADGGWTIPAR"
     gene            complement(8069..9727)
                     /locus_tag="VGA15_00505"
     CDS             complement(8069..9727)
                     /locus_tag="VGA15_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9586211.1"
                     /translation="MSENRFDPVAHARSMRASGFWVDKSFDEFLQQTVAATPGKLALL
                     ADRADRADARRFTYAELSDLIARAAAALKCLGVGPRDVVSVQLPNWWQFAVIALAAFR
                     VGAVVNPLMPIFREHELGYMLDFAETKLLVVPKLFRGFDHEAMGQSLRPRLPKLQHVV
                     VVDGEGATSFDHALLSGSERLGPPPIGEIGALLADQMAVLMFTSGTTGSPKGVMHCLN
                     TLLACNIALAGRFGLDANDTMLVCSPLGHMTGFAAGMLLGLKIGASVIFQDVWEPKRG
                     IAIMANEGVTYSAGAATFLADMCEAVAAGAPKPKRLRKFLCAGAPIPPALIDRVYREL
                     DLNVCSLWGMTESLSSTLTEPERALDKSSKTDGRPLDGVAVKVLRMDGSPAPVGEKGL
                     LKVRGAQMCLGYYKREDMEPFDAEGWFDTGDMAYMDDEGYIRIDGRIKDIIIRGGENV
                     PVFDIENLLFKHPSVLSAAIVGYPDPRLGERACAFVVLRPGQTLDLADVQALMAEHKV
                     AKQYWPERVEIVADLPKTPAGKVQKFQLREIAKAFAEPPRKANA"
     gene            9967..10986
                     /locus_tag="VGA15_00510"
     CDS             9967..10986
                     /locus_tag="VGA15_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9586212.1"
                     /translation="MKAAVLERRGAEGVSWRDFPDPAPAPGESVLKVAAASLNRVDLY
                     MRDNGAGITHALPQVMGVEAAGEIVEAEPGSGLKAGMKAILFSEAYCGRCRYCLAGDQ
                     PLCENVKIMGEHRHGGFAEYIAMPSRCFLPLPDDADLVAAGALMTGHLTAWRMLFGKR
                     TLQPGERVLIVGIGGGVAVACLQLAKLAGAQVFVTSSRDAKIERAIALGASGGVNYQR
                     DRVSNAILEMSNGGVDMVIDSVGEASWGESLRSLRRGGRLVTCGATTGSNPPAELQRV
                     FIRQLEIYGSTGGSVSEFHQLLDVFNRGLVKPVVDATFKMADIRPAFDRLASGTQFGK
                     ISLVA"
     gene            complement(11070..12278)
                     /locus_tag="VGA15_00515"
     CDS             complement(11070..12278)
                     /locus_tag="VGA15_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586213.1"
                     /translation="MRKAFTGLGFGALFSTALSVATAPTVAFADDTIKIGVLLIDSGP
                     LAGLKDSQIKAVNLAIEQINGAGGAAGKKLEASFITYPGTPDTAVDGATRAVQKDGAI
                     FITGMDTSAVSPALAAKLPSLKALMVEVFAQADGLTGKGCSANYFRVNVSDSMIMGAV
                     KEFLKDQGLRKWDIIAVDYAAGRDAADKFKSLIASQGGSIGKTLFVPFGTPDFGAKIS
                     ELGSDPAEGLFVTIFGSDAINLAKQQQQFGLFKKYKMVLGNSFVIPQTLPAQGDAVLG
                     VYQTLGFVPGFPGAEAEAFVKAYKEKYNGELPPYTGADQYTAIQLMAAAINKANSTDI
                     NAVRTALAGLKADTVFGNVEVRAGDHQTVRPIAINQIVAGPDGKPAYQVKKIEPGPDI
                     IPPVDPACKM"
     gene            complement(12307..13002)
                     /locus_tag="VGA15_00520"
     CDS             complement(12307..13002)
                     /locus_tag="VGA15_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586214.1"
                     /translation="MSALRIEMLVAGYGASTVLHGVDLTVDDGESVALVGRNGAGKST
                     LLLSVFRETNVISGEIWVHDNRIDRMPGYMAAKLGISIAPQGRRILPNLTVKENLLLS
                     RATGRAGPWDLRAVYDLFPGLEERSNRPGTMLSGGQQQMLAIGRALMANPSLILLDEP
                     SEGLSPVLIDGLVGALNTIRKAGTGVLVVEQHLNLVKRATDRFIILAKGEVVGSGPTG
                     TIGAPENQALMAL"
     gene            complement(12999..13697)
                     /locus_tag="VGA15_00525"
     CDS             complement(12999..13697)
                     /locus_tag="VGA15_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9586215.1"
                     /translation="MLKIEKLSVSYGSVRALIEVDLEVRGDGALHGVIGPNGAGKSTL
                     LDAVCGRRRPTAGTVRYRDEDITRRTVAWRRRQGMARSFQRTSVFQDLTVYEQLHLVA
                     QHLGDERLDDVIEVMDLADYLDQKAGRIAYGVQRRVDVALALIGRPHLLLMDEPGAGL
                     SAAETLRLFEHLRDLVRERKIAAVVVEHDVDAVFACCGTVTVLDLGRHLATGTPQEIR
                     ADHRVVAAYLGTAA"
     gene            complement(13699..14748)
                     /locus_tag="VGA15_00530"
     CDS             complement(13699..14748)
                     /locus_tag="VGA15_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9586216.1"
                     /translation="MNDRTTTAESPRAAAIGAFGGQSSRMPLTIVAVAIVFVVGAVAP
                     TRFAGDFVLRLANEGLLLGMLALSVAFLMSQAGLVALGAASVYGGAGLLFAIAMSQWD
                     LTPMSAVCASFLIIVVYATVLGALIVRTNPLAFMMLTLAAGEMISHGVMLEGLRDYTG
                     GADGLVVNFKGSILGMEAADFANPARFWLVAWVAAWLAGLALWTVGRSRLGAILRAVR
                     ENEERMRFSGFDTYLPRLIAYVLVNVVAAVSGLLHVLNAGFVSPESLGLSVSTNALVA
                     ALMGGIAGSVGPILGGLIFCFAQDEFGARGLTQLLTGIAIVLFIVVFPRGVVGGLWSV
                     VQLQARALSGRRETS"
     gene            complement(14745..15602)
                     /locus_tag="VGA15_00535"
     CDS             complement(14745..15602)
                     /locus_tag="VGA15_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9586217.1"
                     /translation="MIALLIANSLALASLLIMLASGLALIYGLRDVINFGHGAIYMLG
                     AYLGYTVALVGGFWLALVVVPILLSFLGIAFEYLALRPLQSRSHIEVALVTLGLGIIL
                     GQIIIFFYGGVARSVDAPEVLSGSVTVMNLSYPVYRIFLIVMGLGSCALLALWLRFTS
                     SGLYVRAVSQDPSVARMMGINADRLSLLVTSLSAAFAGIAGVLAGPYLSIDPGMDVTM
                     IVNCLIIVVIGGTGSIGGAIVAALLFGLVQVAGSVFLPNIAALVPYILLMAVLLIAPR
                     GIGRGRVLN"
     gene            15962..16819
                     /locus_tag="VGA15_00540"
     CDS             15962..16819
                     /locus_tag="VGA15_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9586218.1"
                     /translation="MNLEHVIARRFEPAYQSYDWRDAALYALGLGMGDDPLDEDELAY
                     VYEGRDQRAVPSMCVTLGWPPLWIAEPQTGIAWTQALHGEQRLELHRPLPAQGSIRAD
                     HRVSAIEDKGPTRGALLYFDTELFDTTTGERLASLRATEFLRGDGGCGKPPNEIAKLH
                     ANASPIASIDYRTSRQAALLYRLVSRDYMPIHADPAIARDAGFDKPISHGLNTMGLAC
                     RAILKHFAPGRPERLGAMAVRFVSPAFPGDTIRIEMFDGGDAIRFRAWAVERQVLVLD
                     RGNAGLRGG"
     gene            complement(16871..18043)
                     /locus_tag="VGA15_00545"
     CDS             complement(16871..18043)
                     /locus_tag="VGA15_00545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX9586219.1"
                     /translation="MGASTGTALAALKIGGRVAFERLARPKARRFNDVPCSPYAVTAE
                     WLTAVLCSKTPGAIVTQVEVKPASAGTHERHQLMVSYNEEGRRAALPVSIFTKSLPSI
                     VTRMIGGFNGTARVEGHFYTQLRPQLEIEAPLCYHSAYDRRTFAAIHLLEDMVAAKGA
                     TFCNHKTYVSRAMADDMIDLLASLHSRFYGDPTLGERYRWLASYPRWFTIGAQKMGTE
                     YYTRKAFDAAAHVIPAEVLARRDDVWPATMRALALHESEPQGLIHSDVHIGNWYRTAA
                     GQMGLCDWQCLSRGHWSRDFAYAVTASLTPDNRRSWERDLLARFVERFEEKTGVRPDF
                     DLSFRHYRQQIVHALAMWTITLCHSRLLPNMQPEETTLTMIERITTAMADLDSIDS"
     gene            complement(18213..19403)
                     /locus_tag="VGA15_00550"
     CDS             complement(18213..19403)
                     /locus_tag="VGA15_00550"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9586220.1"
                     /translation="MSKPLTGIRVLDLSKVLAGPLCAQYLGDLGADIIKVETVGQGDE
                     TRGWPPFPAAGLGTVFLSANRNKRSIAVDMKTEKGREIVHALAKSADVAIESFGTGVA
                     ERLSIDAATLRSLNERLIHCSISGFGRSGPLKNSPGYDVILQAFCGIMSMTGDEDGGY
                     IRSPISPIDQMTGVHAFSAILALLYAREKTGKGGEIQVSLFETALGLLGYNLQTFWER
                     GVQPPKCGSSHESLCPYQAFEAADGPIMIGVANDNLWRKFCAVAGLDAIVDDPKFRTN
                     ADRVAHRKETIGHVQSAIAQYSAAHWNGALAGVGVPCAPINSIAQLLDHPHTRASGIV
                     VEYEHQTAGRLKGVGHPVLINGAERQAGLPPPVLGQHTDDVLGELGLSSEMIGELRRD
                     RVIG"
     gene            complement(19466..20773)
                     /locus_tag="VGA15_00555"
     CDS             complement(19466..20773)
                     /locus_tag="VGA15_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9586221.1"
                     /translation="MTVLGKLQQFRAERTENNVLHLVFDMPGRAMNVFSNAAIAELAV
                     FSEWLRGSDVNGVVIRSGKPSAFCAGADLGELGLAYDMIMAAPAERRDRAAFDHFFPL
                     SLALRGIETAGKPVAAAIAGLALGGGGELALAAHHRVMVDDPKAAFGLPESLVGLLPG
                     AGGTQRLPRLAGIEKALPVLLEGARLSGQAAITAGVIDQLVAPGEEVAAAERWVLSHA
                     AASQPWDRPGWRPADVDRVAAIIERKRAAVLAETLGHYPAPLAILDCVSQGLPQPFDD
                     AIGTEMRIFSKLIQRREPRNMIRTLFLGRLDHDRLRKAGGIPAPVEQAVTAVSEALQA
                     DGNADAVLANAFARAGFRTPASPAAEFDGVIAQAAFWLEAEPVTDGKRALRERLERAF
                     SVANKWRDRLSEDERRAADYLLVKNCGFPAYIGGAFSSAQLGT"
     gene            21025..>21920
                     /locus_tag="VGA15_00560"
     CDS             21025..>21920
                     /locus_tag="VGA15_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9586222.1"
                     /translation="MIERTIFREEHHIFRETVRRFVDREIVPFHAQWEKDGIVPRELW
                     LRAGAEGLLCCTVPDQYGGMGLDYLFDVVVFEELWRSGASGPGFLIHTDLVATYLLSF
                     GTEAQKCHWLPKMVSGEAIGSLGMTEPHAGSDLKAIRTRAVRDGDDFVINGQKVFISN
                     GQLCDFVVLATKTDGSAGAKGVTLFIVESSRPGFQRGRNLEKIGMKAQDTSELFFEDL
                     RIPCTNMLGQEGKGFSLMMTKLSQERLAQAIRSATVVETVLDWTVEYTSDRSVFGQKL
                     ANFQNTQFKLADLKTKATVARV"
BASE COUNT         4241 a         6945 c         6640 g         4094 t
ORIGIN      
        1 ttcgactatc cgctatcgag ccgactcgac gagaatgacg cggtcttcat catggacaag
       61 gtgctggtgc cttgggaaaa cgtattcgtc tacggcgacc tcgagaaggc aaacaacttc
      121 tttccgcgca ccggcttcct gccccgcttc gtcgtgcatg gctgcacgcg gctcgcggtc
      181 aagctcgact tcattgccgg cctgctgctc aaagccaccg aagccgccgg caccaaggac
      241 tatcgcggcg tgcaggcgaa cgtcggcgag gtaatcgcct ggcgcaacct gttttgggga
      301 ctttcggatg ccatggtccg cgatccgaag ccgtggatcg gcgactatgt cttgccgaat
      361 atggatcccg gcaatgctta tcagatcatc gccacgatgg cttatacgcg ggtcaagtac
      421 ctcatcgagc agaccgttgc gtcgggtctc atctatctca acagccacgc gcgcgatttc
      481 aagaatccgg aaatccggcc ctatctcgat cagtatctgc gcggctcgaa cggctacaag
      541 gcggaagagc gcgtcaagct gctcaagctg ttgtgggact gtttggcgag cgagttcggc
      601 ggccggcatg agctttacga aatcaactac ggcggctcga ccgaggagat ccgccgctac
      661 tgcctgttcg gcgcacaagc ctccggcaac gccgataaat tcaaaggatt cgccgaggaa
      721 tgcatggccg agtacgacct ggatggctgg aaggtgcccg atctcagcga tccaggcgag
      781 ctcagctatc acgtcatgcg cggagccgga taagtcacag cttagatccg gcaaacgtct
      841 ggctttctct gaccgttcat tgcgaacggc cgctttgcgc cactggcgga cattcgccca
      901 tgcatgtagg tcgatacccc ggctatccga ccttgcgagg ctcttgacta acttcggtcg
      961 gactgcgatt cgggttcagc gatcccaggc tcggataggg gacgcatcat ctttcgcaag
     1021 ttgaccgact atcagttggg actctatgcg tcgagcgatt acctccgcgc caacgagccg
     1081 atcgaggaca ttcgagacct gcgtcaacac cacttcattg gatacatcga ggacctgctt
     1141 tttgcgcccg aactaaacta tctcccggca ctgacgaaaa acatcgcggc gaggatcaaa
     1201 agtgccaatc tgaatgcgca gttgaacacc tgcctttcgg gagcaggatt ggcggtccta
     1261 ccgagtttca tgacacgcga tctgccgcag cttcatcgtg tgcttcccga acacactagt
     1321 ttgttgcgca ccttctacat gcagatgcac gaggatagtc gacaaatcgg cccggtacag
     1381 gaaacggcca aatttattct tcagcaggtt cggatcaatc gcgatctgtt caatcccgat
     1441 cactaggctt cgcgttgctc gaaaccgcca acacatcttc cgctgtgatc gccacgaggt
     1501 ttcgaaacca aaatggcaaa accaccatcg ctgtaatcgc tgagatgaaa cgcgataacg
     1561 gcgaggtcgg atccatcctt ttggtctaag atgcaggtcg ggccgatgtt cgctttgcgc
     1621 taacggcggt cgttgatctt ttgaggtaac cggcgtgtcg tttgacgttt ggaggcgtca
     1681 ggttcagtcg cccttgcaat caaatcgatg ccctgcaagc tgaagcggtt accccttcgg
     1741 gagggatcac gcttagacaa gcttgggagc aataccggga cgccccactc ggccgtttag
     1801 caaggggcaa agtggagtgg tgttcggtgc agaagcccag atagtcacgc ctgctccatc
     1861 gcgccggctg cttccagcgc ggcgacttgg tcaggcggaa tgccccaatc tgcgagcgcc
     1921 tgccggctgt cgcgcccggg cgccggcgtt ggccagcgaa gcttcgacgg ggtgcgagag
     1981 aaacgcggcg ccgggctggg atgcagcagc ccgtcgaaca ttgcgtagac ctcacgggcc
     2041 tgcatttgcg gatgctcggg cgcttcgtcg atcgtcagta caggcgcaag acaggcgtct
     2101 cgccccgcgg cagccgcgac ccattcgtcg cgtgtccgca tcgcgaagat tttcgcgaaa
     2161 cgctcgcgca tttgcggcca cgccgtgcgg tcgttctggt ccggaagatt ttcgccgtcg
     2221 atgcctatca ccttcatgag attggcgtaa aaatgcggct cgatcgctcc caccgcgaca
     2281 aacttgccgt ccccggtcgc atagctgcga tagaacgggg cgccgccgtc cacgatgttg
     2341 tcggcgcgcc gctcggtcca gcttccctgc tgacgaaagg cctggaacgc cgacatcaaa
     2401 tgcgtgacgc cgtcgatcat ggcgacgtcg atcgtctgtc cgcgacccga tcggcgtgct
     2461 tccatcgccg cagcgagcag gccgaccgtc agatagagcg ctccaccacc aagatcggcg
     2521 acaagattga gcggcggcgg cggcggccgg tcggcgtcac cgagacagtg cagcgcgcct
     2581 gtgagggcga gatagttgat atcgtggccc gcctcctgcg ctatgggccc cttctgcccc
     2641 cagcccgtca tgcgcgcgta gacaagacgc ggattgaccg cgaggcagac ctcgggacca
     2701 agcccgagcc gctccatcac tgtgggacga aacccttcaa tcaacgcgtc cgcctccgtc
     2761 atgagtttca gcacggtcgc tactgcctgc ggctgcttaa ggtcgaacgc aaccgagcgc
     2821 ttgttacgat tgtagaagtc gaatcgcggc ggaaggtccg gctcgacccc cggcctggcg
     2881 atgcgatcga tgcgcaggac gtcggcgccg agatcgccca gcagggctcc agcgaaaggc
     2941 gcgggtccga ttccgcccat ctcgacgaca cgaaagccat ccaacggtcc cgtcacgatg
     3001 aaatctcctg ttttcggaaa tcggaaagcc ctttgccgga agccgcgagt ccgtcgagcc
     3061 aggacgtcga agcgaaccgc ccgctgtgcg ctttcgcaag acaccggcag agcgcgacca
     3121 attctccgag accgaaatcc tcgacccagc gggcgacgcc gccgagtggt tttgggaaac
     3181 cgaggccgaa aaccgaagcc gcgtccgcgt cgtcggcgct ttcgatcacg ccgtcttcga
     3241 ggcagcgcag aacctcgcgc gcttgggccg ccagcaaacg cagccgcacg ctcccctcat
     3301 cgggctgcgc ggcggcggac ggatacagat cggctagtcc cagccagagc gcgcgcggcc
     3361 cctcattcag cccctggtgc gaggcctcgg cgttgcgccc gcacaagacg accgcgaagc
     3421 ctgcggaggc gaccgcctgc gctatgccga gtcccatcag gcccgtgcca acgacgccga
     3481 caggccccgc gatctcagcg gtggaatcgg acgacgtcat cgatatgcgc ccggccgacg
     3541 cgcgcggcgt tggctttgat gctgcggtcc tcgtagccga acgaaatgcc gaacagcagt
     3601 ttgcaattgg cgggaatgcc gagatgttcg cggacgattt cgggaaacag cccgagcgcg
     3661 ccctgcgcgc aggacgcgat gccccgcgca accagcgcta acatcaaggt ctgcgcatac
     3721 atgccgatat cggccgcctc gcgcgcgtcg aacggttccg gcatgaagat caatacggcg
     3781 tgcggcgcgt cgaagaaggc gtgattgcgg atataggcca tcttgcggcc gaccacgtcc
     3841 cgtcgctcga ctcccatcgc accatacaat ttctgcgccg cgtccacctg gcgctcgcgg
     3901 tagattccgg tgaatttacc atcggccggc cagtccggct tgatcggttc gtcgcgcatt
     3961 cccgcagcga ccagcgcccc gcgaagccgt ttcaacgcgt cacccgacac cacatgcggt
     4021 gtccagggct gcacgttgca attggatggc gaccattgcg cgagctcgaa tacctcgcgc
     4081 agcgtcgcct ccggcacctc gtcggacaga aagccgcgca ccgaacgacg gtggcggatc
     4141 gcgtcgtcga cgttcatggc gggccgttcg tgcatgctca tcgctgttcc tcgatcaacg
     4201 cgacgccgcg cttcgcatag gcccgcgcca gatgacgtcc gacgcgcgcg gcatccggca
     4261 acacgccgct accgagatcc ccgcgcgcgg cgacgccggc gctgcctacc gcgccgcgaa
     4321 acattgcaaa cacctgatgg aatcgtgtca gccgctcctt tgcgcccgac aattcgtaat
     4381 aaagttcaag ataatcgtgc tcggacggaa cgcccatctc atcgagcggc aggccgagca
     4441 atcctccgtt ctcgtccggc gccatgcgcc aagcctgact gttgaacgca agatcgacca
     4501 gaggatggcc gagcgttgaa agctcccagt cgagcacgcc gatgacgtgc ggttcttttt
     4561 cgtggaacat cgcgttagcg atgcggaaat cgccgtggca aagcgcgatg gtctcgcttt
     4621 cgggcagccg ctcggcaagc caatgcacca tcctgtccag ggctgggttg tcgtcgtcgc
     4681 cgcggcggaa ctgcgtccac tgcttcgacc agcgattgag ctggcgggcg aaatagtttc
     4741 cgggcttgcc gaagtcgccc agcccgagcg cctgataatc gagtttgtga atggcggcga
     4801 gcgtggcgca catcgaccga aacagctcgg cacgctcgcc gcgcgtgagg ccaggcgtcg
     4861 cgaagtcgtt aaagacacgg ccgtccagcc attccatgag atagaacggc gtaccgacga
     4921 tctcgtgatc ctcgcagtag ctatagggct tcggcgtcgg aacggcgctc ccttgcagcg
     4981 ccgtgagcac ccgatattcg cggtcgatcg catgcgccga cggcatcagc acattgttgg
     5041 gctgcttgcg cagcacagcg cgccactcgc cgcgggtgac aaagtaagtc gggttcgaca
     5101 tgccgccgtg cgtgcgttcg actcgcgttg gatggacgcc gccgagccag cccttcagat
     5161 attcgtcgag ccttgcgacg tcgaaatcga taccacggtc ggccccgttc gactccgtct
     5221 ccgtcttcac tggcttgctc gcttgccgct aaagttgaac aaatattgcg ccacgcgccg
     5281 gatctgcact tcctcggtac cctcggtgat gcgataccga cggtggtggc ggtagatgtg
     5341 ctcgaacggt agcgcgcggc tgtagccgat gccaccgtgc acctgcatcg cccggtccgc
     5401 cgcctcacaa cacagccggt tggcgcggaa gttgcagatc gacaccatgt cgctgatctc
     5461 cagcggattc tggcgatcca tctgccaggc cgtgcggaag atgaagttgc gcaccagttc
     5521 gtattcggcc cacaactcga ccagagggaa ctgaatcgcc tgcttcttgg cgagcggctc
     5581 gccgaaggcg acgcgtgact ttgcgtattt gaccgcctcc tggatacaat atcgcgccgc
     5641 ccccgcgctc gccgcggcct ggcgcacgcg gttctcgtgc acaaagcgct gcgcgacgac
     5701 gaggccttcg ccctccttgt gcaggatcgc gctatcgggc acccgcacgt tcgtcagttc
     5761 cgtctccgcg tgatcgctgg gcatattgaa agtccagtga ttgtagagaa cgttgtggcc
     5821 gggcgtgtcg gtcggaacga tgaatgcgct gatgcccgtg gcttcgcccg gcttgcccga
     5881 cgtgcgcgcg aagacgagat tggcatgcgc gcgggcaacc tggctgttcc agcgcttgcg
     5941 gccgttgatg atccaatgat cgccgtcgcg cacgcccgtc gtctccagcc atgttgcgtc
     6001 gctgccgtgt cccggttcgg tcaggccgaa ggagagatgc tttttccccg tgatgatgcc
     6061 ctcgatatag gtcttctgct ctttggtgcc gtagtcggcg agcgccggaa tgatcgggaa
     6121 gttgccgacg atcgacgact cgtcctgcag atcgttgtgc aggccgagac ccttggcggc
     6181 gaggtgctcg cggattgccg cgatcgcgag gttggaggcg gcgtggccgc cgcatgattt
     6241 cggcagacca tagcgcagat gccccgcctt gtcggcgcgg cgctccattt cgccgatcag
     6301 cgcgcgccat tccgtgcgcg ggcgcccatc gtcatcccag tcggttcgcg cattttcgcg
     6361 gcgatgatcg aagaattgca tattctcgcg ctcgagcggc ttgatctcgg cctcgataaa
     6421 ggcgtccagc tcgacaagct tgtcggtgat ttccctgggc agttcgaagt ccatggcgcc
     6481 tctccctggt gctctgtttc gttgaagatc gtttctagag cgcaatccgt ccgtatgggt
     6541 gcgtgctgct gacgccgccg tcgaccgaga gcaattgtcc gtcaacatag gaggaatcgt
     6601 cgctggccag aaatgcagct gcggccgcga tctcctcgga acgtcccggg cgcttcatcg
     6661 gcgtgacgtg gccgagcttc gcttcaacgc cacgcgcgcg ggcatcctcg aataccttgc
     6721 gggtcagttg cgtttcgacc agtcccggca gcacggcatt gacgcgcacg ccagttccgg
     6781 caaaggcgtt ggccgccgtc tgcgccaggt tattgaccgc cgccttgctc gcgctgtacg
     6841 aggtcgcgcc cgcattggcg cgcagcgacg cggccgatga ggtgagaatg atcgagccgt
     6901 ggcgctgggc cgtcatgtga cgaccggcgt atttgatggc gaggaagcaa ctgatcgtgt
     6961 tgacgcggta gacttccgac cattcctcca ccgtctgctc cagcagcggc gtgtttgtcc
     7021 cggtgacgcc tatatttgca aagaagactt cgagcccgcc gaactccgac acgcagcgcc
     7081 cgaccatcgc agccacgttt tcctcgatcg atgcatcgac gatcaacgca gatgccatcc
     7141 ccccttcgcc tcggattgtc gctgccgttt cctcggtatt gccagcccgg ccaacgacca
     7201 gtacagaagc gccctcacgc gcgaaacgga tcgcgctcgc gcgaccgatt ccgctgccgc
     7261 cgcccgtcac gaccgcacgc ttgccttcca gtcgcttcat gccgcttctc ctatctggca
     7321 gggatcgtcc agccaccgtc ggccgtgatg acggcgccct ggatgtagct cgcgtcatcg
     7381 cttgcgagga acgcggccag cgaagcgatt tcttccggcg cgccgaagcg cccgctcggc
     7441 atctcggcct gtcgtgttgc gagcgtcgct tcatcgaatt gcgcgatcga agcaggagtg
     7501 gcgatcatgc ccggcgcgat aacgttgaca cggatcttct tcgcactcag ctcaatcgcg
     7561 gcggcacggg aaaggccgac gacgccggcc ttcagcgctg aataggcaat cgaatccttg
     7621 gccggatgaa acgcggacac ggagctcagg ttcacgatcg agccgccgcc gcgtcgctcc
     7681 atctgcgggg ctacggcctg cgtcgtccag atcaggccgt gcacgcccac agcgaacatg
     7741 cgctcgaccg tctcaatctc gatttgcgga agcggctcaa agcgcgccca gaccgcattg
     7801 ttgaccagta cggcgaccgg cgcggcaaac tcgctttcaa tccgcgcgaa cagagcgtgg
     7861 acatcgtcgc gcttgccgac gtcgacctca taagccctgg cttcgcaccc cttgtgcctc
     7921 aactcgttga ctgccggttc gagacggcgc gcgctgacgt cgacacaggc gacgcgtccg
     7981 ccttcccggc cgaagcgctc ggcgatcgca aggccgatcc cgcgcgcggc accggtgacg
     8041 acgcacacgc gatcctttag gcgggaattc atgcgttggc cttgcgtggc ggttcggcga
     8101 aagccttcgc tatctcgcga agctggaatt tctgcacctt gcccgccggg gtcttcggca
     8161 gatccgcgac gatctcgacc cgttcgggcc agtattgttt cgcgactttg tgctctgcca
     8221 tcagggcctg cacatcagcc agatcgagcg tttgtccggg acgcagcacc acgaatgcac
     8281 aggcgcgctc tcccaatcgc ggatccggat atcccacgat cgcagccgaa agcacggagg
     8341 ggtgcttgaa cagcaagttc tcgatatcga ataccggcac gttctcgccg ccgcggatga
     8401 tgatgtcctt gatgcggccg tcgatgcgga tgtagccctc gtcgtccatg taggccatat
     8461 cgccggtgtc gaaccaaccc tcggcgtcga aaggctccat gtcctcgcgt ttgtaatagc
     8521 caaggcacat ctgcgcgccg cgcactttga gcaggccctt ctctccgacc ggcgccggag
     8581 atccgtccat gcgaagcacc ttcactgcga cgccgtcgag cggacgaccg tccgtcttcg
     8641 aggatttgtc gagcgcccgc tcgggctcgg tcaacgtgct cgacagcgat tcggtcatgc
     8701 cccacaagga acagacattg agatcgagct cgcgatagac gcgatcgatc agtgcgggcg
     8761 gaattggcgc gccggcgcaa aggaacttgc gcagccgctt aggctttggc gccccggcag
     8821 cgaccgcctc gcacatgtcg gcgagaaacg tcgccgcgcc ggccgagtag gtaacacctt
     8881 cgttggccat gatcgcgatg ccgcgtttcg gctcccacac gtcctggaag atgaccgatg
     8941 cgccgatctt caggcctagc agcataccgg cggcgaagcc ggtcatgtgg ccaagtggcg
     9001 agcagaccag catcgtgtcg ttcgcgtcga ggccgaagcg cccggcgagc gcaatgttgc
     9061 aggccagcag cgtattgagg caatgcatga cgcctttggg cgagccggtg gtgcccgacg
     9121 tgaacatcag caccgccatc tgatcggcca gaagcgcgcc gatctcgccg atcgggggcg
     9181 ggccgagacg ctcgcttccg gacagcaatg catggtcgaa gctggttgcc ccctcgccgt
     9241 cgacgacgac cacatgctga agtttaggca gccggggtcg cagcgattga cccatcgcct
     9301 cgtggtcgaa tccacggaac agcttgggta cgaccagcag cttcgtctcg gcgaagtcga
     9361 gcatgtagcc aagctcgtgt tcgcgaaaga tcggcatcaa gggattgacg acggcgccga
     9421 cccggaacgc ggcaagcgcg atcaccgcga attgccacca attgggcaac tgcacggaga
     9481 cgacgtcacg aggaccgacg ccaaggcact tcaatgcggc ggccgcgcgc gcaatgagat
     9541 cgctgagttc cgcgtaggtg aagcgcctcg cgtcggcgcg atcggcgcgg tcggccagca
     9601 gcgcgagctt tcccggcgtg gccgcgacgg tctgctggag aaattcgtcg aagctcttgt
     9661 cgacccagaa tccgctagcg cgcatcgatc gcgcgtgggc gacgggatcg aatcgatttt
     9721 cgctcatgcg ttacctccat gacttaaatc gcggcgcttg agggcctttt tcgttagcta
     9781 gtgtactagt tttgcgatgg accgcaacac caaaatcggt cccccgttcg cccacgaaca
     9841 cgtgggacgc gcgccagacc gcggaaatgt tcgcggcact taatggggcg caaaggacga
     9901 cgtatatgat aaacgtgtgt tcgaacatcc gattatgcga cggatgggcc ggaagggaga
     9961 ggcaaaatga aagcggcagt tctggagcgg cgcggagccg aaggcgttag ttggcgggac
    10021 ttccccgatc cggcccctgc ccccggtgag tccgtgctca aggttgccgc ggcgtcgctc
    10081 aatcgcgtcg atctctacat gcgcgacaac ggggccggga tcacgcatgc gttgccgcag
    10141 gtcatgggcg tggaggccgc gggcgagatt gtcgaggccg agcctggcag cggattgaag
    10201 gcgggcatga aggccattct cttttcggaa gcatattgcg gcagatgccg ctattgcctc
    10261 gcgggcgatc aaccgctgtg cgaaaatgtg aagatcatgg gcgagcatcg ccatgggggt
    10321 ttcgccgaat atatcgccat gccgagccgc tgtttcctgc cgttgcctga cgacgccgac
    10381 ctggtcgcgg cgggtgcgct gatgaccggc catctcaccg catggcgaat gctgttcggg
    10441 aagagaacgc tgcaacccgg cgagcgcgtt ttgatcgtcg gcatcggcgg gggcgtcgct
    10501 gtcgcttgcc tgcaactggc gaaactagca ggggcgcagg tcttcgtcac ctcgtcgcgc
    10561 gacgcgaaga tcgaacgcgc gatcgcgctg ggcgcaagcg gcggcgtcaa ctatcaaagg
    10621 gaccgggtat cgaacgcgat cctggaaatg agcaacggtg gggtcgatat ggtcatcgac
    10681 agcgtcggcg aagcaagctg gggggaatcg ctgaggtcac tgcggcgcgg cgggcggctt
    10741 gtcacctgcg gcgcgaccac tggctccaat cctccggctg agctgcaacg ggtgttcatc
    10801 cgccaattgg aaatctacgg gtcgaccggt ggaagcgtta gcgaattcca ccaactgctc
    10861 gacgtgttca accgcggcct ggtgaagccc gtcgtcgacg caactttcaa gatggcggac
    10921 attcgtccgg cgttcgatcg actcgcgtcg ggaacgcagt ttggcaagat atcgctggta
    10981 gcgtgagagg cataccacga actgacgtgg cttcgcaaag gcggttcggc gccgacgtcg
    11041 gaacttgatc gacatcggcg ccgggtctgt cacattttgc aggcgggatc gaccggtgga
    11101 atgatgtccg gtcctggctc gatcttcttg acctggtaag ccggcttgcc gtccggaccg
    11161 gccacaatct gattgatggc gatcggccgc acggtctgat ggtcgcctgc gcgaacttcg
    11221 acgttgccga acaccgtgtc ggccttgagg ccggcaagcg cggtccgcac cgcgtttatg
    11281 tcggtcgaat ttgccttgtt gatcgcggcg gccatgagct ggatcgcggt gtactggtca
    11341 gcgccggtgt agggtggcag ttcgccgttg tacttttcct tgtacgcctt cacgaaggct
    11401 tcagcctctg cgccgggaaa acccggcacg aagccaagcg tctgatagac ccccagcacc
    11461 gcgtcgccct gcgccggaag cgtttgcggg atcacgaagg aattgccgag caccatcttg
    11521 tacttcttga agaggccaaa ctgctgctgt tgcttggcga ggttgatggc gtcgctgccg
    11581 aaaatggtca cgaacagccc ctcggcgggg tcactgccca actcggagat cttggcgccg
    11641 aagtccggcg ttccgaacgg aacgaagagg gtttttccaa tcgagcctcc ttgcgacgca
    11701 atcaacgact tgaatttgtc cgccgcgtcc cggcccgctg catagtcgac tgcgatgatg
    11761 tcccatttcc tgagcccctg gtccttcagg aactccttga cggcccccat gatcatggag
    11821 tcgctcacat tgacccgaaa ataatttgcc gagcatccct tgccggtgag accatccgcc
    11881 tgagcgaaca cttccaccat cagggctttg agcgatggaa gtttggccgc gagagcaggc
    11941 gagaccgccg acgtgtccat cccggtgatg aagatcgctc catccttttg gacggcgcgc
    12001 gtggcgccgt ccaccgcggt gtcgggcgtg cccggatagg tgatgaaact ggcttccagt
    12061 ttcttgcccg cggccccgcc ggcgccattg atctgctcga tcgccaggtt gaccgccttg
    12121 atctgagagt ccttcaggcc ggccagtggc ccactatcga tgagcaggac gccgatcttg
    12181 attgtatcgt cggcgaacgc gacggtcggt gcggtcgcga cggacagggc tgtcgaaaac
    12241 agcgcgccga agcctaagcc ggtaaatgct ttcctcatgt ttctctccct cgttttctga
    12301 ttctgatcac aacgccataa gcgcctggtt ctcaggcgca ccgatggtgc cggtcggtcc
    12361 tgacccgacg acttctccct tggccagaat gatgaatcga tccgtcgcac gcttgaccag
    12421 attcaaatgc tgctcgacca ccagcacccc ggttcccgct ttgcggatcg tattgagcgc
    12481 cccgaccaac ccgtcgatga gaacgggcga aagaccttcc gacggctcgt cgagaaggat
    12541 gaggcttgga ttggccatca gcgcccggcc gatcgccagc atttgctgct gcccgccgct
    12601 cagcatggtg cccggccggt tcgaccgttc ctcgagcccc gggaacagat cgtagacagc
    12661 tctcagatcc caaggccctg ctctgccggt cgccctgctg agcagcaaat tctctttgac
    12721 ggtcagattg ggcaggatac ggcgtccctg cggcgcaatc gagatcccga gctttgcggc
    12781 catatagccg ggcattcgat cgatccggtt gtcatgcacc cagatttcgc cggagatgac
    12841 gttcgtctcg cgaaacacgg agagcagcaa tgtggatttt cccgccccgt tgcggccgac
    12901 caaggccacc gattccccgt catcgacggt aaggtcgacg ccgtgcagca ccgtcgaggc
    12961 gccatagccg gcaacaagca tctcgatgcg caacgcgctc atgcggcggt tccgagatag
    13021 gcggcgacga cgcgatggtc ggcgcgaatt tcttggggcg taccggtcgc gagatgccgg
    13081 ccaagatcga gcaccgtgac tgtgccgcag caagcgaata cggcgtcgac atcgtgctcc
    13141 accaccacgg ctgcgatttt tcgctcgcgc acgaggtcgc gcagatgctc gaacaggcgg
    13201 agcgtctccg ctgcagacag accagctcct ggttcgtcca tcagcagaag atgcggccga
    13261 ccaatcaagg ccagcgcgac gtccactctg cgctgcacgc cataagctat gcgtccagcc
    13321 ttctggtcga gatagtcggc caggtccatc acttcgatca catcgtcgag ccgttcgtcg
    13381 ccgaggtgtt gcgcgaccag gtgaagttgc tcgtagacgg tgagatcctg gaacacgctc
    13441 gtgcgctgga acgatcgcgc catgccctgc cgccgccgcc atgccaccgt gcgccgcgtg
    13501 atatcctcgt cccgatagcg aacggttccc gcggtcggac ggcggcggcc gcagaccgcg
    13561 tcgagcagtg tggattttcc ggcgccgttt ggtccgatca cgccgtgaag cgcgccatcg
    13621 ccacggacct ccagatcgac ctcgatcagc gcccggaccg atccatagct caccgacagc
    13681 ttttcaatct tgagcatgtc aggatgtctc cctccgcccg ctgagcgctc gcgcctgcag
    13741 ctgcaccacg ctccacagtc ccccgacgac gccgcggggg aagaccacga tgaacaaaac
    13801 gatggctatg ccagtcaaaa gctgcgtgag cccgcgcgcg ccgaattcgt cttgcgcgaa
    13861 gcagaagatc agcccgccca ggatcggccc cactgagcct gcaatgccgc ccatcagggc
    13921 cgcgaccaac gcattggtgc tgacagaaag acccaggctt tccggcgaaa cgaaaccggc
    13981 gttaaggacg tgcagcagcc cggagacagc ggcgacgacg ttgacgagca cataggcgat
    14041 cagccgcggc agataggtgt cgaatcccga gaaccgcatg cgctcttcgt tctcgcgcac
    14101 cgcgcgcaag atcgcgccga gacgggagcg gccgacggtc caaagcgcca atccggcgag
    14161 ccacgccgct acccaggcca ccagccagaa gcgcgcaggg ttcgcgaaat ccgcggcttc
    14221 catgccgagt atcgagccct tgaagttgac gaccaggccg tcggcgccgc cggtatagtc
    14281 ccgcaatcct tccagcatga cgccgtggct gatcatctcg ccggcggcaa gcgtgagcat
    14341 catgaaagcg agcgggttgg tgcgcacgat aagcgcgccg aggaccgtcg cgtacacgac
    14401 aatgatgaga aaggaggcgc ataccgcgct catgggcgtc aggtcccatt ggctcatggc
    14461 gatggcgaac agaaggccgg cgccgccata tacgctcgcc gcgccaagcg ccaccagtcc
    14521 agcctgactc atcaggaagg cgacgctgag ggcgagcatg cccaacagca agccttcgtt
    14581 ggcaagccgg agcacgaagt cgccggcgaa gcgtgttggc gcgaccgcgc caaccacaaa
    14641 cacgatagcc acggcgacaa tcgtcagcgg catgcgcgag gactgaccgc caaacgcgcc
    14701 gatcgcggcg gcgcgggggc tttccgcggt cgtcgtccgg tcattcaatt gagcaccctg
    14761 ccgcgtccga tgccgcgggg ggcaatcaac agcacggcca tcagcaagat gtaagggacg
    14821 agcgcggcga tgttcggcaa gaacaccgat cccgcgacct gcacgaggcc gaacagcagt
    14881 gccgcgacga tcgcgcctcc aatgctcccg gtgccgccga tcaccacgat aatgaggcag
    14941 tttacgatca tcgtgacgtc cattccgggg tcaatcgaca gataaggacc agccagcacg
    15001 ccggctatgc cggcgaatgc ggcactgaga ctggtcacca gcaggctgag ccgatcggca
    15061 ttgatgccca tcatgcgcgc aacggacgga tcctggctta ccgcgcggac atagagtccc
    15121 gaggacgtga atctgagcca caaggcaaga agcgcgcacg accccaggcc catgacgatg
    15181 aggaagatcc gatagacggg ataggacagg ttcatgaccg tgaccgaacc tgacaggact
    15241 tccggcgcat ctacgctgcg cgcaacgccg ccatagaaga agatgatgat ctgtccgagg
    15301 attatgccga ggccaagggt gaccagcgcg acctcgatgt gcgatcgact ctgcaggggg
    15361 cgcagcgcca gatattcgaa ggcgatgccg agaaaagaga gcaggatggg caccacgact
    15421 aacgccagcc aaaatccgcc gacgagcgcg accgtgtaac cgagataggc gccgagcatg
    15481 taaatcgcgc catgaccgaa attgatgaca tcgcgcagcc cgtaaatcag ggcgagcccg
    15541 cttgcgagca tgatcagcag tgaggcaagc gccagcgaat tggcgatcag cagcgcaatc
    15601 acgctggcgc cctctcgtca tgcgtcaccg gacgaaacgc attcgtccga tgcatcgcca
    15661 gtctgcgcgc ctgctctcgc aaaaggccct cccccgctgc tatgaggcgt ccgcgtcacg
    15721 gtccgaccgc atgcgcgaca ccgatcacaa gttttttctc cgccctaccg ggcagatccg
    15781 ggtccagccc gtattttagt acacatgttt atcatatact tctattacct gcaaacgcaa
    15841 accgtcggtc ggcgtgcgtt gctctgccgc acggaagccg tcgcgccggg ggctcgcttg
    15901 acgaaattag tacgcgtgcg caacgatacg ccggcaagag gacttgagag caccaacggc
    15961 catgaatctc gagcatgtca tcgcgcgccg cttcgagccg gcctaccaat cctacgattg
    16021 gcgcgacgcc gcgctttacg cgctcgggtt gggcatgggc gatgatccac tcgacgagga
    16081 tgaacttgcc tacgtctatg aaggccgcga tcagcgcgcc gtgccgagca tgtgtgtgac
    16141 gctgggctgg ccgccgcttt ggatcgccga gcctcagacc ggcatcgcct ggacccaggc
    16201 cttgcacggc gagcagcgcc tcgaacttca ccgcccattg cccgcccagg gctcgatccg
    16261 cgccgatcat cgcgtgagcg caatcgagga caaggggccg acgcgcggcg cgctgctcta
    16321 tttcgacacc gaattgttcg atacgacaac cggcgaacga ctggcgagcc tgcgcgcgac
    16381 ggagtttttg cgcggcgatg gcggctgcgg caaaccacca aacgagatag ccaagctgca
    16441 cgccaatgcg tcacccatcg ccagtatcga ctaccgcacg tctcgccagg cagccttgct
    16501 ctatcgcctc gtcagccgag actacatgcc gatccacgct gatccggcga tcgcgcgcga
    16561 cgccggcttc gacaagccga tctcgcacgg tctgaatact atgggcctgg cctgccgcgc
    16621 gatcttgaag cattttgcgc cgggaaggcc ggagcgactt ggcgcaatgg cggtgcggtt
    16681 cgtttcgccc gccttccctg gcgacaccat caggattgag atgttcgacg gcggcgacgc
    16741 aattcgcttc agggcctggg ccgtcgagcg acaggtgctg gtgctggatc gcgggaatgc
    16801 cggcctgcgc gggggatagc ggggttcgca ggagcgcgcg atgaagttcg agacgaacgc
    16861 aaagaataca tcaactgtcg atcgaatcca gatcagccat ggccgtcgta atgcgctcga
    16921 tcatcgtgag cgtcgtttcc tcgggctgca tattgggcaa tagccgcgag tgacatagcg
    16981 tgatcgtcca catggcgagc gcatgcacaa tctgctgccg atagtgccgg aaactgagat
    17041 cgaagtccgg tctgacgccg gtcttctctt cgaaccgctc gacaaagcgg gccaacaggt
    17101 cacgttccca gctccgacga ttgtccggcg tcagcgaggc tgtcacggcg taggcgaaat
    17161 cgcgtgacca gtggccgcgc gaaagacatt gccagtcgca caaccccatt tggcccgctg
    17221 ctgtccgata ccagtttcca atgtgaacat cggagtgaat gagtccttgc ggctcgcttt
    17281 catgcagcgc aagtgcgcgc atcgtcgccg gccagacatc gtcacggcgc gcaaggacct
    17341 ccgctggaat gacgtgggca gcggcgtcaa atgccttgcg ggtgtaatac tccgtaccca
    17401 ttttctgcgc gccaatggta aaccaacgtg gatagctggc gagccatcgg taccgctcgc
    17461 cgagcgttgg atcgccgtag aagcggctgt gcagcgatgc cagcagatcg atcatgtcgt
    17521 ctgccatggc gcgcgagacg tacgtcttgt gattgcaaaa cgtcgcgccc ttcgcggcga
    17581 ccatatcttc cagcaggtgg atcgccgcaa atgttcgccg atcgtaggcg gaatgatagc
    17641 acaacggtgc ctcgatctcg agctgaggtc gaagctgcgt atagaaatgg ccctcgacac
    17701 gcgctgtgcc gttgaagccg ccgatcatgc gcgtgacgat acttggcagc gatttggtga
    17761 agatcgaaac cggcagcgcc gcgcgccggc cctcctcgtt gtaggacacc atcagttgat
    17821 ggcgttcgtg cgttcccgcg ctcgccggct tgacttcgac ctgcgtaaca atggctccgg
    17881 gagtcttgct acacaatacc gccgtcagcc attccgccgt aactgcatag ggcgaacagg
    17941 gcacatcgtt gaaccgccga gcctttggcc gcgcgagacg ctcaaaagcg acgcgtcctc
    18001 cgattttcag cgctgcaagg gcagtccctg tcgatgcacc catgatccaa ctccccgttc
    18061 agcacccacc ggcaactgca gcgaccatga tcgatccggc ccgatcaaac tcaggttctc
    18121 ggctacatcg acggcgattt tgaaaatccg tacgctatat tacgatctgg atcaataatc
    18181 gaatgatgaa ggctggacgc gccgtcccaa tatcaaccga tcacgcgatc ccgccttaat
    18241 tcgccgatca tttcagatga cagacccagt tcgcccaaga catcatcggt gtgctgccca
    18301 agcaccggcg gcggcagccc tgcttggcgc tccgccccgt ttatcagaac ggggtgacct
    18361 acgcccttca gccggcctgc ggtttggtgc tcgtattcga caacaatgcc gcttgccctt
    18421 gtgtgcgggt ggtcaagcaa ttgagcaatc gaattgatgg gcgcgcaggg aacgccgacg
    18481 cccgcaagcg cgccgttcca gtgcgcggcg gaatactgag caatcgctga ctggacatga
    18541 ccgatggttt ctttgcgatg cgcaactcga tccgcgttcg tcctgaactt cggatcatcg
    18601 acgatggcgt ccaatccggc gacggcacaa aacttgcgcc acagattgtc gttcgcaacc
    18661 ccgatcatga ttgggccatc ggcagcctcg aaggcctgat aaggacaaag cgactcgtgg
    18721 ctcgaaccac atttcggagg ctgaacgccg cgctcccaaa acgtctgcag attatagccg
    18781 agcaggccca gcgcggtttc gaagagcgag acctggatct cacctccctt ccctgttttc
    18841 tcccgcgcat acagcagcgc cagaatggcg ctaaacgcgt gtacgccagt catctggtcg
    18901 atcggagaga tcggactacg gatatagccg ccgtcctcat cgccggtcat tgacatgatc
    18961 ccgcaaaatg cctgcaggat cacgtcatag cccggtgagt ttttcagcgg ccccgaacgg
    19021 ccaaatccgg aaatactaca gtggatcagg cgctcgttca gagagcgaag cgttgcagca
    19081 tcgatgctca gcctctcggc gacgccggtc ccgaagcttt cgatggcaac gtcggcagat
    19141 tttgccagcg catggacaat ttcgcgcccc ttttccgttt tcatgtcgac ggcgatgctt
    19201 cgcttgtttc ggttggcact gagaaagaca gtgccaaggc cggccgccgg gaaaggtggc
    19261 cagccgcgcg tttcgtcacc ctggccgacg gtctctacct tgatgatgtc ggcaccgaga
    19321 tcgccgagat attgggcgca gagtggacca gccaggactt tcgacaggtc gaggacgcgt
    19381 atgccagtca agggctttga cactgctttt ctcccgattg gagttgcgca agccgcgatg
    19441 cagcacgttt cgaagatggc gccaatcagg tgccgagctg agcggaggaa aaggcgcctc
    19501 cgatataagc gggaaagccg caattcttga ccagcagata atccgccgcg cggcgctcat
    19561 cctccgacag ccggtcccgc catttgtttg cgacggaaaa cgcccgctca agacgttcgc
    19621 gcagcgcgcg tttgccgtcg gtgacaggct ctgcctctag ccaaaacgcc gcctgtgcaa
    19681 tgacgccatc gaattccgct gccgggctcg cgggtgtgcg aaagcccgcg cgggcgaagg
    19741 catttgcaag cacggcatcc gcgtttccgt ccgcttgcaa tgcctcggaa acggcagtca
    19801 ctgcttgttc gacaggtgca ggaatgcctc ccgcctttcg aaggcggtca tgatcaagcc
    19861 tgccaagaaa cagcgtcctg atcatgttgc gcggttcacg ccgttgaatg agctttgaga
    19921 atatccgcat ctcggtgccg atcgcgtcat cgaatggctg cggcaatccc tggctaacgc
    19981 aatcgagaat agcgagcggc gccggatagt gcccaagcgt ttccgcaaga acagccgcgc
    20041 gcttccgctc gatgattgca gctacacgat cgacatcggc cggccgccag cccgggcggt
    20101 cccagggctg cgacgccgcc gcatgggaca acacccatcg ttccgctgct gccacctctt
    20161 cgccaggagc aaccaactga tcgatcacgc cggcggtgat ggcggcctgc ccggacagtc
    20221 gcgcaccttc caacaggacc ggaagagcct tttcaattcc ggccaggcga ggcaaccgct
    20281 gggttccccc cgcccctggt aacagcccga caagtgactc cggcaagcca aacgcagcct
    20341 tgggatcgtc gaccatgacc cgatgatgcg cagccagcgc cagttccccg cccccgccaa
    20401 gcgccaggcc ggcaatcgcc gccgcaaccg gtttgcctgc ggtttcgatt ccgcgcagcg
    20461 ccaggctgag aggaaagaag tggtcgaacg ccgcgcgatc acgcctctca gccggcgcgg
    20521 ccatgatcat gtcgtaggcc aatcccaact cgccaagatc ggcgccggcg cagaatgccg
    20581 atggctttcc cgatcgaatg accacaccat tcacgtcact tccgcgcagc cactcgctga
    20641 agacagcgag ttcggcaatg gccgcattgg agaacacgtt catcgcgcgg ccgggcatgt
    20701 cgaaaacgag gtgcagaaca ttgttttcgg tgcgttccgc ccggaactgc tgcaacttcc
    20761 ccaacaccgt catggtattc tccgcgatag cgtcccgcct atagcatatc gtatatctgt
    20821 gtactagttc aaagcaatgg attttgcagc cccggaaaag cgcttgagtg cgattgccgc
    20881 gattatctgc cgagctggtt ccgaagttct ggcgcgggca gcgccgactc agtggtgcaa
    20941 aaaagctctc ggctcccagc ttgcttttta cctgcctccc gcatatggta cactaactaa
    21001 ctaaattgat gcggacagat agcgatgata gagcgaacca ttttccgcga agagcaccat
    21061 attttccggg aaacggtgcg gcggtttgtc gatcgcgaaa tcgttccgtt tcatgcccag
    21121 tgggagaagg acggtattgt gccacgggag ctttggctca gggccggcgc agagggtcta
    21181 ctttgctgta ctgtgccgga tcagtacggc ggcatgggcc ttgactacct cttcgacgtt
    21241 gtcgtgttcg aggaattatg gcggtcgggc gcgagcggcc cgggatttct gatccatacc
    21301 gatctcgtag cgacctatct tctctccttt ggaaccgagg ctcaaaagtg ccattggctg
    21361 ccgaagatgg tgtcgggcga agccatcggc tctctcggca tgacggagcc tcacgcgggc
    21421 agcgacctca aggcgatccg gacgcgcgcc gttcgcgacg gcgacgactt cgtcatcaac
    21481 ggtcagaagg tcttcatctc gaacggtcaa ctctgcgatt tcgtcgtgct tgcgaccaaa
    21541 accgacggca gcgccggcgc caaaggcgtg acgctgttca tcgtcgagag cagccggccg
    21601 ggatttcagc gcgggcgaaa tctcgagaag atcgggatga aggcccagga tacttctgaa
    21661 ctgttcttcg aggacctgcg tatcccctgc accaatatgc ttggacagga gggcaaaggg
    21721 ttctcgttga tgatgacgaa gctttcgcaa gaacggcttg cccaggctat ccgctcggca
    21781 accgtcgtcg aaaccgtcct tgattggact gttgagtata cttcggaccg cagcgtgttc
    21841 ggtcaaaaac tcgcaaactt ccagaatacg caattcaagc ttgccgatct gaagaccaag
    21901 gccacagtcg cccgcgtttt
//
LOCUS       DASWGM010000018         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2080320,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000018 DASWGM010000000
VERSION     DASWGM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080320"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VGA15_00565"
     CDS             complement(<1..512)
                     /locus_tag="VGA15_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX9586223.1"
                     /translation="MPPPRKRADLLLVERGLFESRARAQAAIEAGLVTANDKPVTKPS
                     EAIPADAALQAQPAHPFVSRGGVKLAGALQQFPIDIEDHVCLDVGASTGGFTEVLLAN
                     GASLVFSIDVGREQLHPSLRGHPKIISMEETDIRNFEGKRLPMRPDVVVIDVSFISLK
                     AVLPVALSLAA"
     gene            complement(536..1108)
                     /locus_tag="VGA15_00570"
     CDS             complement(536..1108)
                     /locus_tag="VGA15_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEX9586224.1"
                     /translation="MKIYLAGPDVFLHDAVDIGRRKVELCKRHGLIGLYPLDNAIDPA
                     AQDVSLQIFRGNETMMMESDAIIANLTPFRGPGADPGTVYELGYMAGRGKLCLGYSNH
                     PSSYAERVREFTDVSSRDGRLVDALGLTVEDFGLADNLMMIHALELHGYALVTPPQMP
                     ADIWHDLTAFETCVRMAAERQALPHPPASN"
     gene            complement(1241..3166)
                     /gene="dxs"
                     /locus_tag="VGA15_00575"
     CDS             complement(1241..3166)
                     /gene="dxs"
                     /locus_tag="VGA15_00575"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX9586225.1"
                     /translation="MTVFSKTPLLDTIRTPDDLRRLKVDQIRQVADELRQETIDAVSV
                     TGGHFGAGLGVVELTTALHYVFDTPRDRLIWDVGHQAYPHKILTGRRDRIRTLRTGGG
                     LSGFTKRTESDYDPFGAAHSSTSISAALGMAVARDLSGGKNNVIAVIGDGAMSAGMAY
                     EAMNNAGAMNSRLIVILNDNDMSIAPPVGAMSAYLSRLYSGKTYRTLREAAKQINKRL
                     PKILANRANRVEEYSRGFMMDGGTLFEELGFYYVGPIDGHNLDHLLPVLKNVRDMETG
                     PILVHVVTQKGKGYGPAEASADKYHAVVKFDIATGAQAKAKPNAPSYQNVFGQSLVKE
                     AEKDEKIVGITAAMPSGTGIDIFNKAFPDRTFDVGIAEQHAVTFAAGLATEGYKPFCA
                     IYSTFLQRGYDQIVHDVAIQSLPVRFAIDRAGLVGADGATHAGSFDNAYLGCLPNFVI
                     MAASDEAELVHMVATQVAINDRPSAVRYPRGEGRGIEMPEFGVPLEIGKGRIVREGTK
                     IALLSFGTRLAECEKAADELAAHGLSTTIADARFMKPLDVDMVLQLARDHEILLTIEE
                     GAIGGFGSHVMQTLAENGMLDGGLRMRSMVLPDEFLDHDTPNAMYARAGLDAKGIVAK
                     VFEVLGKDFKTETVKLA"
     gene            complement(3516..4826)
                     /locus_tag="VGA15_00580"
     CDS             complement(3516..4826)
                     /locus_tag="VGA15_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586226.1"
                     /translation="MRVQHRHIIYVQGYDPRGLAQYYRMFRTELRKFGRLYALTATIS
                     RPQVAPDGETASWTIETKAADWQTRTSYDFLRWEDLIQRDLASPIWRTVFHAMWIYWR
                     LVFGGTIARFWKAHWRFATFISYPHYLLFNEALLSLGLAFGFGKGLEALGIPGLFSMA
                     ASAAVFVAVLGTMLKYTENSTYLLYLLSDTIWTWEFSHRQRPDWDQRIDRFAQYLANV
                     ARTSDAEEIVVVGHSSGSFLGAEILARALKLDPALGRHGPRVVLLTIGGNFPIVGFHA
                     ASKDFRDHLRLLAVEPSIDWIDCQSRKDVMNFYPFDPIEGHGIDVGALRRNPTIVPVR
                     FREIIKPEHYNLFRWKFFRVHFQFVMANERPHAYDFFMIVCGPILLIERMARPEAALA
                     VATGDAAAREWAWKRLETSTSDQADAADLGEMEPKMEPTARRRG"
BASE COUNT         1004 a         1509 c         1591 g          955 t
ORIGIN      
        1 gccgccagcg acagcgccac cggcagcacg gctttcaacg aaatgaaact gacgtcgatg
       61 acgacgacgt ccgggcgcat cggcagacgc ttgccttcga agttccggat atcggtctcc
      121 tccatcgaga tgattttggg atggccgcgc agcgagggat gcagttgctc gcgcccgaca
      181 tcgattgaaa acaccagact ggcgccgttg gccagcaata cctcggtgaa gccgccggtc
      241 gatgcgccga cgtcgagaca gacgtgatcc tcgatgtcga tcggaaactg ctgcagcgcc
      301 cccgccagtt tgacgccgcc ccgcgagaca aaggggtgcg caggctgcgc ctgcagcgcc
      361 gcatcggcgg gaatggcctc ggatggtttt gtgaccggct tgtcattggc ggtgaccagg
      421 cctgcctcaa tcgcggcctg ggcgcgggcc cggctttcga acagaccgcg ctcgaccagc
      481 aacaggtccg cgcgtttgcg gggagggggc atgttcgcct cgcggctcgg caaattcaat
      541 tcgatgcagg cgggtgcggc aaggcttgcc gctcggccgc catgcgcacg caagtctcga
      601 acgcggtcaa atcgtgccag atgtcggccg gcatttgcgg aggcgttacc agcgcgtagc
      661 catgcagctc gagcgcatga atcatcatca gattatcggc caacccgaaa tcctcgacag
      721 ttaatcccag cgcatcgacc aggcgtccat cgcgcgaact cacgtcggtg aattcacgga
      781 cacgctcggc atacgacgag ggatggttgg agtagccgag acagagcttg ccgcgtcctg
      841 ccatatagcc gagttcgtag acggtcccgg ggtccgcgcc cgggccgcga aacggcgtga
      901 ggttggcgat aatggcatcg gattccatca tcatggtttc gttgccgcga aatatctgca
      961 aagacacgtc ctgggctgcg ggatcgatgg cattgtccag cggatacagt ccgatcaatc
     1021 catgacgctt gcataactcg accttgcgcc gcccgatatc gacggcgtcg tgcaggaaaa
     1081 cgtcggggcc agccaggtaa atcttcatct ttaattgcgc ggattctgcg cgtgattgaa
     1141 ggtcttgcga cggcaagctg acaaaatggg gcggcaaagc gccacccgcc agcagcagcc
     1201 aacgccggtt caggcgcggg ccgccggcgc agccgagttg tcaggccagt ttcaccgtct
     1261 cggtcttgaa atccttgccc agcacctcga ataccttggc gacgatgccc ttggcgtcga
     1321 gaccagcgcg ggcgtacatc gcattcggtg tgtcgtggtc gaggaactcg tcgggcagca
     1381 ccatcgaccg catccgcaag ccgccgtcga gcatgccgtt ttccgccagc gtctgcatga
     1441 catgggagcc gaatccgccg atcgcgcctt cctcgatggt gaggaggatt tcgtggtcgc
     1501 gcgcgagttg cagcaccatg tcgacgtcga gcggcttcat gaagcgcgcg tcggcgatgg
     1561 tggtggacag tccatgcgcc gccagttcgt ccgcggcctt ctcgcattcg gcaagacggg
     1621 tgccgaacga cagcaacgcg atcttggtgc cctcgcgaac gatgcgccct tttccgatct
     1681 caagcggtac cccgaattcg ggcatctcga tgccgcggcc ttcgccgcgc ggatagcgaa
     1741 ccgcgctcgg acggtcgttg atcgcgacct gggtcgcaac catgtgcacc agttcggcct
     1801 catcggaggc cgccatgatg acgaaattgg gcaggcagcc gagataggca ttgtcgaacg
     1861 agccggcatg ggtggcgccg tcagcgccca cgagcccggc acgatcgatc gcgaagcgca
     1921 ccggcaggct ctggatcgcg acgtcatgga cgatctggtc atagccgcgc tgcaggaagg
     1981 tcgaatagat cgcgcagaac ggcttgtagc cttcggtcgc aagacccgcg gcaaaggtca
     2041 ccgcatgctg ctcggcgatg ccgacgtcga aggtgcggtc ggggaaagcc ttgttgaaaa
     2101 tgtcgatccc ggttcccgac ggcatcgccg cggtgatgcc gacgatcttc tcgtcctttt
     2161 cggcttcctt gacgaggctt tggccgaaca cgttctgata cgagggggca ttcggcttgg
     2221 cttttgcctg cgcgcccgtg gcaatgtcga acttgaccac cgcatgatac ttgtcggcgg
     2281 acgcttccgc cgggccgtag cccttgccct tttgcgtgac gacatgaacc aggatcgggc
     2341 cggtctccat gtcgcggacg ttcttcaaga ccggcagcaa atggtcgagg ttgtggccgt
     2401 cgatcggacc gacataatag aagccgagtt cctcgaacag cgtgccgccg tccatcatga
     2461 agccgcggga atattcttcg acacggttcg cgcggttggc gaggattttc ggcagacgct
     2521 tgttgatctg cttggccgcc tcgcgcagcg tgcggtaggt cttgccggag taaagccgcg
     2581 acaggtaagc cgacatcgct ccgaccggag gcgcgatcga catgtcgttg tcgttgagaa
     2641 tgacgatcag gcgggagttc atcgcgcccg cattgttcat ggcttcatag gccatgccgg
     2701 ccgacatcgc gccgtcaccg atcacggcga taacattatt cttgccgccg gagaggtcgc
     2761 gcgccacggc catgccgagc gctgcggaga tcgaggtgga ggagtgcgcc gcaccgaacg
     2821 gatcgtaatc gctttcggtc cgcttggtga agccggacag cccgccgccg gtgcggaggg
     2881 tgcgaatgcg atcgcggcgg ccggtcagga tcttgtgcgg ataggcctga tggccgacgt
     2941 cccagatcaa acggtcgcgc ggggtgtcga acacatagtg caacgccgtc gtcaattcga
     3001 cgacgccgag gccggcgccg aaatgcccgc ccgtgaccga gacggcatcg atggtttcct
     3061 ggcgcagctc atcggcaact tgccggattt ggtctacttt gagccggcgc agatcgtcgg
     3121 gagtgcggat cgtgtcgaga agcggcgttt tactgaatac tgtcacggcg atattccaat
     3181 tttgcatgtc gagacaagcc gtccgacgtg agatgggttg cgcactatca gcgcagcgaa
     3241 agcctggaca ccagatgcca gctcggcagc cgaagcctga ttttgaaagc ctagatgcgc
     3301 tccagatcgt tcactgtgat acgtatcact cttggcggct tcacccttcc caagaaattt
     3361 aagaagctat ttgaagcact taccaccgtc caagaccgat ggcagtccgc tttcaacacc
     3421 ccatagcccg cagtgcttta caccaaagct gcggtcaaag ccaacccgcc ggaaccgccg
     3481 gcgcgcgatg gataagggtg gcggagcgcc agatttcaac ccctccgccg cgctgttggt
     3541 tccatcttgg gttccatctc gcccaagtcg gcggcatctg cctgatcgga ggtggaggtt
     3601 tcgagccttt tccaggccca ttcgcgggcg gcggcgtcgc cggtcgccac tgccagcgct
     3661 gcctcgggac gagccatgcg ttcgatcagc aggatcggcc cgcagacgat catgaaaaaa
     3721 tcgtaggcat gaggccgttc gttggccatg acgaactgga aatggacccg aaagaatttc
     3781 cagcggaaca ggttgtagtg ctcgggcttg atgatttcgc ggaatcggac cggcacgatg
     3841 gtggggttgc gtcttaaggc gccgacatcg atgccgtggc cttctatcgg atcgaacggg
     3901 tagaaattca tcacatcctt gcgcgactga cagtcgatcc agtcgatcga aggctcaacc
     3961 gccagcaggc ggaggtgatc ccggaaatcc tttgacgcgg catgaaaacc gacgatcggg
     4021 aaattgccgc cgatcgtgag caggacgacg cgcggaccgt gtcgtccgag agccggatcg
     4081 agcttgagcg cgcgcgccag aatctcggcg cccaaaaacg atcccgaact gtggccgacg
     4141 acaacgattt cctcggcgtc gctggtgcgt gcgacgttgg cgaggtattg tgcaaatcgg
     4201 tcgatgcgct ggtcccaatc cggccgctgg cggtgcgaga attcccaggt ccagatcgtg
     4261 tcggacagca gatagagcag ataagtcgag ttttccgtgt atttcagcat ggtgccgagc
     4321 acggcgacga ataccgccgc cgacgcggcc atgctgaaca ggccgggaat gcccagcgct
     4381 tccaaccctt tcccgaaacc gaacgcaagg cccagcgaca gcagggcttc attgaacagc
     4441 aggtagtgcg ggtaactgat gaaggtcgcg aaccgccaat gcgctttcca gaaccgcgca
     4501 atcgtgccgc cgaacaccag ccgccaatag atccacatcg catggaaaac cgtgcgccag
     4561 atcggcgagg caaggtcgcg ctggatcagg tcttcccatc gcagaaaatc gtaactggtg
     4621 cgggtctgcc agtcggctgc cttggtctcg atggtccagg atgcggtctc gccgtcgggc
     4681 gccacttgcg gacggctgat ggtggcggtg agggcataca gccggccaaa cttccgcagt
     4741 tcggttcgaa acatgcggta atactgcgca aggccgcgcg gatcgtatcc ctgcacatag
     4801 atgatatggc ggtgctgaac gcgcacttac tgctgatccc cggactggtg ccgccgattt
     4861 gaaattcctg caccggttcc cgcaagatcg catcaggaat ttcaaatcga agagcggcac
     4921 tagaatatag agttacgaat gccccgtttt tcgaagttcg tggcagagga tgccgcaagg
     4981 tatcgcgaac ttcgaaaaat ggggctctga cggcggggac tataacaggc cgggtaacgc
     5041 gccaagagct atttcagac
//
LOCUS       DASWGM010000019         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_832514,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000019 DASWGM010000000
VERSION     DASWGM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832514"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGA15_00585"
     CDS             <1..238
                     /locus_tag="VGA15_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586227.1"
                     /translation="ASGALFAFATSFDEVVIVLFMAGPEQRTLPREMFSGIRENISPT
                     ITAAAVILTTVSVILLATMEGLRRRNERLKRNNA"
     gene            complement(421..2128)
                     /gene="ggt"
                     /locus_tag="VGA15_00590"
                     /pseudo
     CDS             complement(421..2128)
                     /gene="ggt"
                     /locus_tag="VGA15_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271111.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(2978..>3272)
                     /locus_tag="VGA15_00595"
     CDS             complement(2978..>3272)
                     /locus_tag="VGA15_00595"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9586228.1"
                     /translation="EKNSETGRAPLNYGIGVHVGDVMYGNIGSRTRLDFTVIGPAVNM
                     ASRLETLTKQLGRTVLLSRAFADSVESDFDLERIGEYPVRGFNDPIELFAYHG"
BASE COUNT          562 a         1003 c         1046 g          661 t
ORIGIN      
        1 ggcgtcgggc gcgctgttcg cctttgcgac cagcttcgat gaggtggtga tcgtgctgtt
       61 catggccggg ccggaacagc gcaccctgcc ccgcgagatg ttcagcggca tccgcgagaa
      121 catcagcccg accatcaccg ccgcggcggt gatcctgacg acggtgtcag taatcctgct
      181 cgccaccatg gaaggcttgc gccgccgcaa cgagcggctg aagcgcaaca acgcctgacc
      241 ggcgtcctta tcccttgcgg cggaccggga gcaccgtccg tccgctggct cttctatagc
      301 tttcgggcat gttcggtcga ccgcagcgtg cttgtgttga cgatgtcgcg tcgtccgctg
      361 cgctcgcgtc gcgatgagat gcacccgccg aagccacctt gttggatcgg gcgatgacga
      421 tcagtagccg gcagccagcc cggtgttgcg gcgcgggtcg ttcgccccgt agaagcgatt
      481 gttgccgacc ggcttgcccg tcagcgacgg cgcgccgatg atgattaccg cgaggtggtt
      541 ggccggttgc ggcggaccga acttgtggcc cttgccctcc aggatcttgc gggtgtccgg
      601 cgacagcgcg taattctcca gattggtcag gtccgggagc cactgctggt ggaagcgcgg
      661 catatcggcg gcttcctgcg cgttcatgtc gtaatcgatg gcgttgatca tcgtctgcag
      721 cacggccgtg atgatgcggc tcccgcccgg ggtgcccacc acgatcaccg gcttgccgtc
      781 cttggtgacg atggtcgggc tcatggagga cagcggacgc ttgccgggcg cgatggcgtt
      841 cgcctcgccc tgtaccaggc catagaggtt cggtacgccg atcttggcgg tgaagtcgtc
      901 catttcgtcg ttcagaagca cgccggtctt ggccgccgtc acccgggccc caaaccagtc
      961 gttcagcgtg taggtgaccg acaccgcgtt gccgtccttg tcggcgatcg aatagtgcgt
     1021 ggtgttgctg ccctcatgcg gctcgacgcc cggtttgatg tccttggaca cggccgcctg
     1081 tttcgggtcg atcgcggcac ggatcctggc cgcgtaattc ttgtcgagca ggcggtcgag
     1141 cgggtttttc acgaagtccg ggtcgccgag atagctgttg cggtcgacat aggcgtggcg
     1201 catggcttcg atctggtagt gcacggcctc cgcggagtgg tagcccagct ccttgagcgg
     1261 atagccctcc agcacgttca ggatctcgca gatgatcaac gccgcccgag ctcggcggcg
     1321 gcgcggagac cacgtggtag ccgcggtaat cgcattcgac gggtgccagc tcacgggtct
     1381 tgtagccgtc caggtcctcc tgcgtgatca ggcctttgcc ggcctggctc gatgccacga
     1441 tggcgctgcc gacccagccc ttgtagaagc cgtcggtgcc ccgcttgctg atttcgcgca
     1501 aggtctcggc gagctcatgc tgcacgagct tctcgcctac ctggaacggc ttgccgttgt
     1561 tgaggaagat ggcggctgac gcaggatcct cctgaaaatc cttggtcgcc gtgagcagca
     1621 tgtcgatgtc gccctggtcg agtgcgaagc cgtcctcggc tagttgaagc gccggcgcaa
     1681 tcagctccgc gcgcttcatg gtgccgtact tctccctagc gtattccatg cccgaaacgg
     1741 agccgggtac gccgacggcg aggtgcccct tggttgacag gcccttgatg acgttgccgt
     1801 ccttgtcgag gtacatgttg gcggtggcac ccttgggcgc cgtctcgcgg aaatccagga
     1861 acgtcttgcg gccatcggca agctggatgg tcatgaatcc accgccgccc aaattgccgg
     1921 ccgccggata gaccacggcg agcgcatacc caaccgctac cgccgcgtcg accgcgttgc
     1981 cgccgcgctt gagcacatcc acacccacca tcgtggccaa gtgctgggcg gaaaccacca
     2041 tcccgttttc ggcggcgacc ggcgccacgg acgccgcgtg gccgggcgcg aaggccgtga
     2101 tgctgatcga cgtgatcgcg gcgaccatcc accgcacgcc tgttctgggg ttttgcatgg
     2161 gagctcctcg gaaacgccgg ccttcgcagc ctaggttgtt attgaagaca tacgtctatc
     2221 agggcgcgcg cccgcgcacc atggggtcat caccgttcgg cgaacaacac gagtgggcga
     2281 tgcgcgaact ttgcgaaatg attacgaaga aaggggatcg gatcggatct cggccgatca
     2341 aatcgatcac gccgcgaggt gctgacaaga tctatgatcg tctcacgctc ggaccgaagg
     2401 gtccaagatt gcgaaccgcg tgcttctctg tcgcaaagct tggcgggtcg tgcatcgact
     2461 ttatcaggag gagttcaaca aggacgtttc gaatccttgg cccggcgtca cgatgaaaac
     2521 cagggtgaag ctgaccaagc ctgcagcaac gagagaacag gtctatatgt tcgcacacgg
     2581 ttgtattgag cgcggcgaag tcgagtgcgg cgaagtccgc ggtcatttgt tttgagtggc
     2641 tccagcgccc ggagaatgtg gttgccggcc atatcaagtg gaccggttac cgtaccgggt
     2701 ccaagccgac catccgaatc gagcaccata agaccggagc tgtggatcga tcacccgctc
     2761 gagtcggggg gttctagtcg ggagaccgct accgcttttc cccacacgac gtaaatctgc
     2821 gatatttcct ccgatcgtcg cctaaactcg gaaacttatc gaatggcagg gtcaaaatcg
     2881 ggtactagca accaccgaga ccggcattcg ctgattgtgg ccgtttgacg agttcgcgcc
     2941 ccttggggcg gctcggcacc tgacgataga tcggcattca gccgtgatac gcaaacaact
     3001 cgattgggtc gttgaagccg cgcaccggat attcgccgat gcgttcgaga tcgaaatcgc
     3061 tttcgacgga gtcggcgaac gctcgggaca gcagcaccgt tctccccaat tgtttggtga
     3121 gggtttcgag ccgcgaagcc atgttgaccg caggaccgat gacggtgaag tcgagccggg
     3181 tgcgcgaccc gatattgccg tacatgacgt ctccgacgtg gacgccgatg ccgtaattca
     3241 gcggcgcacg accggtttcg ctgttctttt cg
//
LOCUS       DASWGM010000020         8795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2357941,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000020 DASWGM010000000
VERSION     DASWGM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8795
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357941"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VGA15_00600"
     CDS             complement(<1..233)
                     /locus_tag="VGA15_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX9586229.1"
                     /translation="MMITRRAFTAALSLTGLAALAGLSPLRLISYAMAQSASDVAKPV
                     SLPDMALGPADASVTITEYASMTCPHCAAFNEAV"
     gene            complement(351..830)
                     /locus_tag="VGA15_00605"
     CDS             complement(351..830)
                     /locus_tag="VGA15_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX9586230.1"
                     /translation="MAKPGPISAKPLSVLLSDVFSDAYAKQGFAARELVTRWGEIAGA
                     EIAAHSEPLKIQWPRPVQGQPQEPATLVLRVEGPMALEIQHASDVILQRVNRFFGWSA
                     VGRLALRQAPLSRRNRPAASRAPDPDAVAKVAETLSSVEDAELRAALARLGASIKRN"
     gene            complement(868..1188)
                     /locus_tag="VGA15_00610"
     CDS             complement(868..1188)
                     /locus_tag="VGA15_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX9586231.1"
                     /translation="MSKSSTTTSRSGLLLNPHPGEILLEEFLKPMGLSQNALAREVHV
                     APRRINEIVLGKRDVTADTDLRLARYFGLSEGFFLGLQMDYDLMQRRREIDRDLKTIR
                     PRAA"
     gene            complement(1160..1444)
                     /locus_tag="VGA15_00615"
     CDS             complement(1160..1444)
                     /locus_tag="VGA15_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9586232.1"
                     /translation="MIQGFADSETELIWSGRRSRKLPPDIQNVALRKLRLLNQARVLG
                     DLRIPPGNRLETLRADRQGQHSIRINDQWRICFRWDEGGPSHVEIIDYHV"
     gene            1537..2658
                     /locus_tag="VGA15_00620"
     CDS             1537..2658
                     /locus_tag="VGA15_00620"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX9586233.1"
                     /translation="MPISTAIKKTKQTVPEDGAARPALLLEWYDRHRRKLPWRPPAGE
                     RADPYSVWLSEIMLQQTGVKTVGPYFLKFLARWPDVDALGRASLDDVLRMWAGLGYYS
                     RARNLHACAVAVRRDHGGAFPDTEEALRALPGIGPYTAAAIAAIAFGRWTMPVDGNIE
                     RVVSRLFAVEEPLPLAKPLIKQLATTLLADTRAGDEKSRAGDSAQALMDLGSSICTPK
                     KPACALCPLNGDCVSRARGDQETFPRKAAKKTGALRRGAAFVVMRGDELLVRTRPEKG
                     LLGGMTEVPGSDWLAVQDDKTALKQAPVLNGIARWHRKVGVVTHVFTHFPLELVVYTA
                     NVAARARAPKGMRWVPVATLRDEALPNVMRKVIAHGLGE"
     gene            complement(2883..4172)
                     /locus_tag="VGA15_00625"
     CDS             complement(2883..4172)
                     /locus_tag="VGA15_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEX9586234.1"
                     /translation="MTVLIAGGGIGGLTLALSLHQIGISAKVFESVSELKPLGVGINV
                     LPHAVRELIELDLHDRLDTAGVRTKELAYFSKRGKPIWSEPRGIEAGYKWPQFSIHRG
                     TLQQILLDAATERLGPENIFTSHHLCDWTETSSGVRADFIDKATGKSAGSHDGALLIA
                     ADGIHSAIRERLYPLEGPPIWNGRILWRGITASDAFLSGRTMVMAGHETLKFVCYPIS
                     KEPDVDGKFQINWVAERHMPPTYQWRREDYNRTAKPEEFLPWFEDWKFDWLDVPGLIR
                     NCPRAYEYPLVDRDPVAQWTFGLTTLMGDAAHPMYPVGSNGASQAILDARIITREILA
                     HGPTNAALLAYEAERRPATSDLVMLNRRNGPEQVMQLVEERAPDGYNAVTDVLSLQEL
                     EDIAANYKRVAGFQVEGLNAKPPIVTMPAPSRLSGAG"
     gene            4396..5220
                     /locus_tag="VGA15_00630"
     CDS             4396..5220
                     /locus_tag="VGA15_00630"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9586235.1"
                     /translation="MSSQHSNTHDVATGHFSSLLKHWRNVRRLTQIELAGDANVSARH
                     LCFLETGRAQPSREMVQLLGSALDLPLEECNALHVAAGFVPPYGDRGLAAENLQPVRQ
                     ALDFILRQQEPYPGIVIDGRWDVRIRNQASRRLFKAFHQSYEMEADIAENAMHVVFHP
                     KGLRQFIVNWDEFAGQMIQILHREVAQGGRVAAQLLDEIMVYPGLPAEWRLPRNPAGS
                     SPVMTMQLRKGDFRLAFFSTFTTLAMPTDAALQKLKIECFYPADSATAAQARELAL"
     gene            5240..5716
                     /locus_tag="VGA15_00635"
     CDS             5240..5716
                     /locus_tag="VGA15_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9586236.1"
                     /translation="MQFAQLNHQSAETMNELGALNARFIHNFVTNDVPSHDAILHPAF
                     INIWPTGQRWDRAAYLKYWATAFDPKIIVYWDVRDELITIVGDVALVRATNKHTRRRD
                     GQEVTGMTMYTDTYLFENGAWQCIQAQLTSVAPEHQPADDTIVSVYIEGKRQERLS"
     gene            6106..6726
                     /locus_tag="VGA15_00640"
     CDS             6106..6726
                     /locus_tag="VGA15_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9586237.1"
                     /translation="MLKLYYAPGTCALASHIALQEAGATYTTERLDFKSNQQNSPEYL
                     AINPKGRVPSLVTDRGILTETPAMLAFIAQRFPQAKLAPFDDAFAFAQVQAFNSYLCS
                     TVHVAHAHKGRGYRWATDEASFADMKRVVPKSVGASFALIERNMLKGPWVIGETYTIC
                     DPYLFTLASWLEGDGVDLSTLPKVADHRKRMSERPAVQKVLAEEKS"
     gene            complement(6811..7734)
                     /locus_tag="VGA15_00645"
     CDS             complement(6811..7734)
                     /locus_tag="VGA15_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9586238.1"
                     /translation="MNLTSLDLNLLVALDALLLEANVSRAAMRIGLSQPAASHALQRL
                     RDVLGDPLLIRVGARMELTPRAQALRGPLAQALDQVRGLFISDDFDAASSERHFRLMM
                     PDLAVELLVPPLMEKIAALAPNVRLDIVPWRGPAIFTAEFARTIDLVISIGNAFKGFH
                     RQLLYTDRDALAVRRGHAIGAKLKRRDVFLHARHIAVVIRGQNEDLIDGWLRAKGLER
                     RIALVVPGYIEALHIVARTDLVAFVPGRLIAALAKPLSLITVTPPLDPGVDEQFMFYP
                     TRAQFDPGSIWLRKLMLETGRGLDRDGRKAA"
     gene            7827..8270
                     /locus_tag="VGA15_00650"
     CDS             7827..8270
                     /locus_tag="VGA15_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9586239.1"
                     /translation="MSAAANKELMQEIFAAAGDPDPAVRDRALFVASLADDAKWVVTG
                     QYSWSGTFTGKEAILGELHGHVRSLLVERARTIAHRFMADGDYVVVEAKGDNLTRTGV
                     RYDNDYCLVFRLENGKIKEVREYCDSVLTENALGRFPEQHGRVAG"
     gene            8409..>8795
                     /locus_tag="VGA15_00655"
     CDS             8409..>8795
                     /locus_tag="VGA15_00655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9586240.1"
                     /translation="MAKPSKEFLSPKTLIPPAGYSHIAKVNSGTIVYLAGQVSSGASG
                     NLIGEGNFEAQVEQVFRNLKIAVEAAGGTMADIVKLNIYLVAEVDQADVPKLRAIRDR
                     YVNIEKPPASTLVVVSRLARPGWLVEI"
BASE COUNT         1673 a         2694 c         2827 g         1601 t
ORIGIN      
        1 acggcctcgt tgaaggccgc gcaatgcggg caggtcatcg aggcatattc cgtgatcgtc
       61 accgaggcgt cggcggggcc gagcgccatg tccggcagcg ataccggctt ggccacatcg
      121 gaggcgctct gcgccatcgc atacgagatc agccgcagcg gcgagagccc ggcgagggcg
      181 gcaagcccgg tcagcgacag ggcggcggtg aaggcgcggc gcgtgatcat caagggtttg
      241 ctcccggatt ggcgcggtca cgcccataga acctgaaaga agacagttcg ctagcttgaa
      301 accaccatcg tggcaatggc gggccgcgtc tcccggtccc ggcggaaccc tcaatttcgc
      361 ttgatcgagg ccccaagccg cgccagcgcg gcgcgcaatt ccgcgtcctc gaccgaggac
      421 agggtttcgg caacctttgc caccgcgtcc gggtccggtg cgcgcgaggc cgcgggccgg
      481 ttccggcgcg atagcggcgc ctgccgcagc gccagccgtc cgaccgcgct ccagccgaag
      541 aagcggttga cgcgctgcag gatgacgtca gaggcgtgct ggatttccag cgccatcggg
      601 ccctcgaccc gcaagaccag cgtggccggt tcctgcggct gcccctgcac cgggcgcggc
      661 cactggattt tcagcggctc ggaatgggcc gcgatctcgg cgccggcaat ttctccccag
      721 cgcgtgacca gttcccgcgc ggcaaatccc tgcttggcat aggcgtcgga aaacacgtcg
      781 ctgagcagga cggacaacgg cttggcgctg atcggaccgg gtttggccat ggcttcctat
      841 aacaccggcc gctcgataga gacacgctta tgccgcgcgc gggcggatgg tcttcaggtc
      901 gcggtcgatc tcgcggcgtc gctgcatcag atcgtagtcc atctgcaagc cgagaaagaa
      961 tccctccgac aggccaaaat agcgggccag ccgcagatca gtgtcggcgg taacatcccg
     1021 tttgcccagc acgatctcgt tgatgcggcg cggtgcgacg tgaacctcac gcgccagcgc
     1081 attctggcta aggcccatcg gcttgaggaa ctcttcgagc aggatttcgc cggggtgcgg
     1141 gttaagaagc agcccgcttc tagacgtggt agtcgatgat ttcgacatgt gacggtcctc
     1201 cctcatccca tctaaaacaa atacgccatt gatcattgat cctgatcgaa tgctgcccct
     1261 gacgatccgc tctaagcgtt tcgaggcgat tgcccggcgg aatgcgaaga tcgcccaata
     1321 cccgcgcctg attcaaaaga cgaagcttcc tcagagcgac gttttggatg tccggcggca
     1381 attttcggct ccggcgtccg gaccagatca attccgtttc ggaatcggcg aagccctgga
     1441 tcatgagatg actataacgt aacgcgttat agttcgcaat ccccaggcag aaccaagctc
     1501 gctggtcatg cagtgattct gttcctaaaa tgagatgtgc cgatatcgac agccatcaag
     1561 aaaacgaagc agaccgtccc cgaagacggc gccgcccgtc cggcgctgct gctggaatgg
     1621 tacgaccgcc atcggcgcaa gctgccgtgg cggccgcccg ccggcgagcg cgccgatccc
     1681 tattccgtct ggctgtcgga aatcatgctg cagcagaccg gcgtcaagac ggtggggccg
     1741 tatttcctga agtttctggc gcgctggcct gacgtcgacg cgctgggccg cgcctcgctc
     1801 gatgacgtgc tgcggatgtg ggccgggctc ggctattatt cgcgcgcgcg caatttgcat
     1861 gcctgcgcgg tggcggtgcg gcgcgatcac ggcggcgcgt ttcccgatac cgaggaagct
     1921 ttgcgcgcgc tgccggggat tgggccttac accgccgctg ccattgccgc gattgccttc
     1981 ggccgctgga ccatgccggt cgacggcaac atcgagcggg tggtgtcgcg gctgttcgcc
     2041 gtcgaagaac cgctgccgct ggcaaagccg ctgatcaagc aattggccac gacgctgctt
     2101 gccgatacgc gcgccggcga cgaaaaatct cgcgccggcg atagtgcgca agcgctgatg
     2161 gatctcggct cctcgatctg caccccgaaa aagccggcct gcgcgctgtg tcccctgaac
     2221 ggcgattgcg tgagccgtgc gcgtggcgat caggagacgt ttccgcgcaa ggccgcgaag
     2281 aagaccggag cgctgcgccg aggtgcggcg ttcgtcgtca tgcgcggcga cgagctgctg
     2341 gtccgcaccc gccccgaaaa gggcctgctc ggcggcatga ccgaagtgcc gggctcggac
     2401 tggctcgccg tccaggacga caagacagcg ctgaagcagg cgccggtgct gaacggcatc
     2461 gcgcgctggc atcggaaggt tggcgtggtc acccacgtct tcacgcattt tccgctggag
     2521 ctcgtagtct acaccgcgaa cgttgccgca cgtgcccgcg cgcccaaggg aatgcgctgg
     2581 gtgccggttg cgacgctcag ggacgaggcg ctgccgaacg tcatgcgcaa ggtgatcgcg
     2641 catgggctcg gggagtaaca tccccacctc atcgcggcgg gtaagtgcag cgaacgcgcc
     2701 gggatctctt ctccctctcc ccgttcttga cggggagagg gttggggtgg ggcgctccgg
     2761 cccgcaaatt ctgcggatgt tgatgagcac tgcctcgccc ctcacccgct cgcaatccgg
     2821 acagcgcaag agcgctgtcg ggagctcgcg gcctctccgc aaagcgcggg gagaggtgaa
     2881 gatcaccccg cgccggaaag ccggctcggt gcaggcatcg tcacgatcgg cggcttggcg
     2941 ttgaggcctt cgacctgaaa gccggcgacg cgcttgtagt tggcggcgat gtcttccagc
     3001 tcctgcagcg acagcacgtc ggtgacggcg ttgtaaccgt ctggcgcgcg ttcctcgacc
     3061 agttgcatca cctgttccgg tccattgcgc cggttgagca tgacgaggtc cgatgtggcg
     3121 gggcggcgct ctgcttcata cgccagcagc gcggcgttgg tcggaccgtg tgccaggatc
     3181 tcgcgggtga tgatgcgggc atcgaggatc gcctgcgacg cgccgttgga gccgaccgga
     3241 tacatcggat gcgcggcgtc gcccatcagg gtggtcaggc cgaacgtcca ttgcgccacc
     3301 ggatcgcggt cgaccagcgg atattcgtag gcccgcgggc agttgcggat caggccgggc
     3361 acatcgagcc agtcgaattt ccagtcctcg aaccacggca agaattcttc gggctttgcg
     3421 gtgcggttgt aatcctcccg ccgccattga taggtcggcg gcatgtgccg ttcggcgacc
     3481 cagttgattt ggaatttgcc gtcgacgtcc ggctccttcg agatcggata gcagacgaat
     3541 ttcagggttt cgtggccggc catgaccatg gtgcggccgg agagaaacgc gtctgacgcg
     3601 gtgatgccgc gccagaggat gcgtccgttc cagatcggcg ggccttccag gggatacagc
     3661 ctttcgcgga tcgcggagtg aatgccgtcg gctgcgatca gcagcgcgcc gtcatggctt
     3721 cccgcggatt tgccggtggc cttgtcgatg aaatcggcgc ggacgccgct cgaggtctcc
     3781 gtccagtcgc aaagatgatg gctggtgaag atgttctccg gccccaaccg ttcggtcgcg
     3841 gcatcgagca ggatctgttg cagcgtgccg cggtggatcg agaactgcgg ccacttgtaa
     3901 ccggcctcga tgccgcgcgg ctcgctccag atcggcttgc cgcgcttgga aaagtacgcg
     3961 agttccttgg tgcgcacgcc tgctgtatcg agccggtcgt gcaggtcgag ctcgatcagc
     4021 tcgcgcaccg cgtgcggcag cacgttgatg ccgacgccga gcggcttcaa ttcggacacg
     4081 ctctcgaaca ctttggcgga aatgccaatt tgatgcaggc tgagcgccag cgtcagtccg
     4141 ccgatgccgc cgcccgcaat aagtacggtc atgatacgcc cctgttattc atggcttgat
     4201 gacatgagag gcggcagggg gcaaccgaga aggtagctcg cgagggcctc gtcgcgggct
     4261 cgcactttgc gtcatgccgg ggtcgaaggt gaatttcttc tttccggaaa cgtcagactt
     4321 gcttcgcctt atgcgcaccg cgacttacgt tcgaggtaat tgatcttctg atctaacagc
     4381 ttaaggctaa ccgtcatgag ctcccaacac tcgaacacgc acgatgttgc gacaggacac
     4441 ttcagcagct tgttgaagca ttggcgaaac gttcgccgcc tcacccagat cgaactggca
     4501 ggcgatgcaa atgtctcggc caggcatctg tgctttctgg aaaccggacg cgcgcagccg
     4561 agccgcgaga tggtgcagtt gctcggaagc gcgctcgatc tgccgctcga ggagtgcaat
     4621 gccttgcacg tcgccgccgg tttcgtgccg ccctacggcg accggggatt ggcggccgag
     4681 aatttgcagc cggtacgcca ggcattggat ttcattcttc gccagcagga gccgtatccc
     4741 gggattgtca tcgatgggcg ctgggatgtc cgcatccgca accaggcttc ccgacgcctg
     4801 ttcaaggctt ttcaccagtc atacgaaatg gaagccgaca ttgccgaaaa cgccatgcac
     4861 gtcgttttcc atccaaaagg cctgcgacaa ttcatcgtga actgggacga atttgccggc
     4921 cagatgatcc agatccttca tcgcgaagtc gcacagggcg gcagggtagc ggcgcaactg
     4981 ctcgacgaaa tcatggtgta tcccgggctg ccggccgaat ggaggcttcc gcgcaatccg
     5041 gccggatcgt ctccggtcat gacgatgcag ctaaggaagg gcgactttcg cctcgctttc
     5101 ttttccacct tcacgacgct tgcgatgccg acggacgcag cactgcaaaa attgaagatc
     5161 gaatgctttt atccggcgga cagcgcgacc gcggcgcaag cgcgcgaact ggcactttga
     5221 caatattcag gagatgaaaa tgcagtttgc ccagctcaac catcaatcgg ccgagaccat
     5281 gaacgaattg ggcgccttga atgcgcgctt catccataac tttgtgacaa acgacgtgcc
     5341 ctcgcacgac gccatccttc atcccgcctt catcaacatc tggcccaccg gccagcgatg
     5401 ggaccgcgcg gcctatctga agtattgggc aaccgccttc gatcccaaaa tcatcgttta
     5461 ctgggatgtc cgcgacgaac tcatcacgat cgtcggcgac gttgcgctgg tgcgcgccac
     5521 caacaagcac acgcgccgcc gcgacggaca ggaagtgacc ggcatgacca tgtacaccga
     5581 cacctatctg ttcgagaacg gcgcgtggca gtgcattcaa gcccagctca catcggttgc
     5641 tcccgaacac cagcccgccg acgatacgat cgtcagcgtt tatatcgagg gcaaacgtca
     5701 ggaacgactt tcgtgacaga tcgcgcgggc caaacattga gcggcggcta cgcggaaagt
     5761 ggccggcaat gtcatcagcc atcgagaaca tcgatgagtt cgctggtcgt cctggttcgg
     5821 aagattctct ccacggttca aatctggcgg gcgcgcgccc actcaaggcg agagctcgct
     5881 tcgcggagcg attgtgaatt gcgggatatg ggaacctgct gggcgtcgat cgctgacgag
     5941 gtcagcaagc cgttctggcg ggcgtgatag gctgagccga acgccgctcc cgaggggggc
     6001 aggcggcacc tgcgaaagcc cgacatcgca ggcgacggcc cggccgcttg ggatggacgg
     6061 caagagagtg tgccgttatt gttcggcttt gccatgagga ccaacatgct caagctctat
     6121 tacgctcccg gaacctgcgc gctcgcttct catatcgccc tacaggaggc cggtgccacg
     6181 tacacgaccg agaggctcga cttcaaaagc aaccagcaga acagtccgga atatcttgcg
     6241 atcaatccga aggggcgcgt gccgtcgctg gtgacggatc gcggcatcct gaccgagacg
     6301 ccggcgatgc ttgctttcat cgcgcagcgt tttccgcagg cgaaactggc gccgttcgac
     6361 gacgctttcg cattcgcgca ggtgcaggcc ttcaacagct atctgtgctc caccgtgcat
     6421 gtggcgcacg cccacaaagg gcgcggctat cgttgggcga ccgacgaggc ttcctttgcc
     6481 gacatgaagc gcgtggtgcc caaaagcgtc ggcgccagtt tcgcgttgat tgaacgcaac
     6541 atgttgaagg ggccgtgggt gatcggtgag acctatacga tctgcgatcc ctatctgttt
     6601 acgctggcga gttggctgga aggcgacggc gtcgatcttt cgaccctgcc gaaagtggcg
     6661 gatcaccgca aacggatgtc ggaacggccc gcggtgcaaa aagtccttgc cgaagagaag
     6721 agctgaaatc ggggccggca aagccaacgg accgcgcgaa cgcgcgcccg atggatggga
     6781 ggcctggcgc gaattgcgcc ttggcccacc ctaggcagcc ttgcgcccgt cgcgatccag
     6841 cccgcgtccg gtctccagca tcagcttgcg tagccagatc gatccgggat cgaactgcgc
     6901 ccgcgtcgga taaaacatga actgttcgtc gacgccgggg tcgagcggcg gcgtgaccgt
     6961 tatcagcgac aacggttttg ccagggcggc gatcagccgg ccgggaacga atgccacgag
     7021 atcggtacgc gcgacgatat ggagcgcctc gatatagcct ggcacgacca gcgcgatgcg
     7081 tcgctccagc cctttggctc ggagccagcc gtcgatcagg tcttcgttct ggccgcggat
     7141 gacgacggcg atgtgccgtg catggaggaa aacatcgcga cgcttcagct tcgcgccaat
     7201 ggcgtggccg cgccgtaccg cgagcgcgtc acggtcggta tagagaagtt ggcgatgaaa
     7261 tcccttgaag gcattgccga tcgagatcac cagatcgatc gtgcgcgcaa actccgcggt
     7321 gaagatcgcc ggaccgcgcc acggcacaat gtcgagccgg acgttcggcg ccaaggcagc
     7381 aatcttctcc atcagcggcg gcaccagaag ctccacggcg agatcgggca tcatcaggcg
     7441 aaagtgccgt tcgctgctgg cggcatcgaa gtcgtccgaa atgaaaagtc cgcgcacctg
     7501 gtcgagtgcc tgcgccagcg gcccgcgcag cgcctgggcg cgcggcgtca attccattcg
     7561 cgcgccgaca cgaatcagca agggatcgcc gagcacgtcg cgcagccgct gcagcgcatg
     7621 gctcgccgcc ggctgcgaca gcccgatccg catcgcggcg cggctgacat tcgcctccag
     7681 cagcagtgcg tcgagcgcga ccaaaagatt gaggtcaagc gaagtcaaat tcatacgctg
     7741 aatatatatc atatttacta tcgattggaa gaatgacgcg caatgcggca atgattggcg
     7801 gaaaactgat caatggagaa gccgccatga gcgcagccgc caacaaggaa ctgatgcaag
     7861 agatcttcgc cgccgcggga gaccccgatc cggcggtgcg cgaccgcgcc ttgttcgtcg
     7921 ccagcctggc cgatgacgcg aaatgggtgg tgaccgggca atattcgtgg tcgggcacgt
     7981 tcaccggcaa ggaagcgatc ctgggcgaac tgcacggtca cgtccgttcg ctgctggtcg
     8041 agcgggcgcg cacgatcgcg catcgcttca tggccgacgg cgactacgtc gtggtcgagg
     8101 ccaagggcga caacctcacc aggacggggg tccgctacga caacgactat tgtctggtgt
     8161 tccggctgga gaacgggaag atcaaggagg tcagggaata ttgcgactcg gtgctgaccg
     8221 aaaatgcgct ggggcggttt ccagagcagc acgggcgtgt tgcgggttga gccgtcgagc
     8281 agtgtttgta cgcgacgtca gcttgctgcg atgaccgaac gcgaattaca ggatatgggt
     8341 acctgccggt ctgacgtcgt ggacgaactc ggcgggccgt tctggctgaa gtgataaggg
     8401 agatcgacat ggccaagcca tcgaaagaat ttctcagtcc aaagaccctg atcccaccgg
     8461 cgggctattc gcatatcgcc aaggtcaata gcgggacgat cgtctacctg gcgggccagg
     8521 tctcgagcgg cgcttccggc aatttgatcg gtgagggcaa ttttgaggcg caggtcgagc
     8581 aggtcttccg caatctgaag atcgcggtcg aagcggccgg cggcaccatg gccgacatcg
     8641 tcaaattgaa catttacctg gtcgcggagg tcgatcaggc cgatgtgccg aagctgcgtg
     8701 cgatacgcga ccgctacgtc aatatcgaga agccgccggc aagcacgctg gtcgtcgtca
     8761 gccggctggc gcggccgggc tggctcgtcg agatc
//
LOCUS       DASWGM010000021        11907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1387083,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000021 DASWGM010000000
VERSION     DASWGM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11907
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387083"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VGA15_00660"
     CDS             complement(<1..1086)
                     /locus_tag="VGA15_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX9586241.1"
                     /translation="MTAAPGVLALQRALVWLVGASGAIVFIEPSPYELVTLAATVIFF
                     ATGLRLRLVFMPLLLLLFLVNIGYCISAASLMDKPEVANWIATSCYMAFTVIFLAMVL
                     SEDTAARLDMISRGLTVGALIAATAGIAGYFNLVPGGHDLLTLYDRASGTFKDPNVLA
                     AFLILPALFALQSVVSDDFRKSFRSAIALGIMALAILLAFSRAAWGGLAITSSFMLAL
                     MVLTSRSRAQRSRIIMMTVVTAIIVAALIAVLLSFDSIAEMFKQRASFDQSYDEGRFG
                     RFGRHILGAGMALDLPFGIGPLQFHKYFPEDTHNSYLNAFMSGGWIAGVCYPALVFIT
                     VIMGFRHIFVPVPWQRAYLAIFAAFLGT"
     gene            complement(1220..2758)
                     /locus_tag="VGA15_00665"
     CDS             complement(1220..2758)
                     /locus_tag="VGA15_00665"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX9586242.1"
                     /translation="MEPINARSILDAAAAATAVRPPTERRQRLSHAALAVANQKVRRA
                     YSPIVIAGAVRVADFVLVSLVGVALYLGYVVPLSGFHWEYIAAILGMTATAVLCFQAA
                     DIYEVQVFRGQLRQMTRMISSWAFVFLLFIGASFFAKLGGEVSRLWLSAFFFVGLSAL
                     LAERLFLRALVRGWARDGRLDRRTIVVGSDQTGEKLIEALKTQEDSDIHVLGVFDDRN
                     DSRVLDTCAGSPKLGKVDDIVELARRTRVDLVLFALPISAETRILEMLKKLWVLPVDI
                     RLSAHTNKLRFRPRSYSYLGKVPTLDVFEAPITDWDLVMKWLFDHVVGAVILLLASPL
                     MALVALAVKFDSPGPVLFRQKRFGFNNERIDVFKFRSLYHDQADPLAVKVVTRNDSRV
                     TRVGRFIRKTSLDELPQLFNVVFKSNLSLVGPRPHAVQGKLQSRLFDEAVDGYFARHR
                     VKPGITGWAQINGWRGEVDSEEKIQKRVEFDLYYIENWSVLFDLYILLKTPPALMTKN
                     DNAY"
     gene            complement(2885..4018)
                     /locus_tag="VGA15_00670"
     CDS             complement(2885..4018)
                     /locus_tag="VGA15_00670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9586243.1"
                     /translation="MSMMADQPLRILHAVRTPVGGIFRHILDLANGQVDRGHQVGLIA
                     DSLTGGERAEAALAAIAPRLALGVHRLAIRREPFPTDVLVWARFMRLIRRLKPDVLHG
                     HGAKAGAFIRLKTGSKATIRVYTPHGGSLHYRLNTIKGALYSRLERALTDSTDLFLFE
                     STFARDTYQRTIGTPKGLVRCVFNGVTAEEFDPVVKADDATDVVYVGEFRHIKGADLL
                     IDAVARLHADGKPVTLTLAGDGEELEALKAQIQRLGLTEAVRFIGHVKARYGFSKGGV
                     LVVPSRGDSMPYVVIEAAAAGIPMVAANVGGIPEIFGSHTEALFAPNIVSAMADAIEI
                     ALEDPEAAQARAKSLRERIFLHFSQKAMVEGVLAGYREAFAKR"
     gene            complement(4021..4707)
                     /locus_tag="VGA15_00675"
     CDS             complement(4021..4707)
                     /locus_tag="VGA15_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX9586244.1"
                     /translation="MRMSAPVAGLQPQSDLDSLAYGQPTNPALEPSSRSDVADSGGAI
                     SALRSSFAAAPRRADAPVPVAYAAAPMPPGHDAVYRLDAGDKLRVVVYGQEGLTNSYA
                     IDAGGAITMPLIGAVPARGRTPAGLASAISAKLRNGYIREPSVAVEIEAYRPFFILGE
                     VAAPGQYPYIPNMSVESAVAIAGGFSPRALRDRVTLTHTDHSGSSRFVVPLGTALGPG
                     DTILVGERWF"
     gene            4958..7180
                     /locus_tag="VGA15_00680"
     CDS             4958..7180
                     /locus_tag="VGA15_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HEX9586245.1"
                     /translation="MRLPFWRAGKDKAATAEPVVQTAIPTPAPATDKPAVASESGDLD
                     LRALGQALVRNRGWVIVPTALAAVLSIAAVNLVTPRYKSEARILIDGRENIFLRPNGE
                     RSEERNALDAEAVTSQVQLLLSRDLARDIIKKNKLAERPEFDPVLQGISPVKSLLALF
                     GIGRDPFSLTPEERVLDAYFDRFTAYAIDKSRVIVIEFQSRDPDLAARVANSIAEGYL
                     VLQQGARREQAKSAGQWLSGEIDNLRKKVAEAESRVEDFRSKSSLFVGTNNTTLSNQQ
                     MGELNTQLNNARALKSDAESKARLIREMLQSGKPIEASEVLNSELTRRLSEQRVTLRA
                     QLAEQSSTLLDNHPRIKELRAQLADLDRQLREEASKISRSLDNDSRIAGGRVDGLSAS
                     LEQLKKQATSSNSQDVQLRALEREAKAQRDLLESYLAKYREATTRENIDAAPADGRII
                     SSASVSNTPAYPKKLPIVLIATLATLMLSTGAIATGELLRMTAPRTTAALTSPPAPVE
                     RTPAPAAAAVAAVAAEPAPLPKEAHPDIAAGPAEIERLADDVHKAGDAARKVTILGTA
                     ANESITLTALTLARLLARDSRVVVVGLSASSSAISAASVDPDAPGFAELMLGETSFSH
                     VITKDRSSRVHLVSAGRPGSDRALLQSPRLALAIDALLRVYDHVLLDAGTASDLPAEL
                     LTAQARAVVVPDPSMTADARTSMCDQLRAAGCFTGLTMLSEPVQPSDAVEPGPRVVAA
                     "
     gene            complement(7218..8411)
                     /locus_tag="VGA15_00685"
     CDS             complement(7218..8411)
                     /locus_tag="VGA15_00685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9586246.1"
                     /translation="MTMAAAIESRTADELAWSKSSRIAGVDILHDLVQAEAIWRALED
                     PQQFSTPYQRFDFLGPWQRQVGEREGLSPFIVIAYDAERRPLLLLPLALSHQCGVRTA
                     CFMGGKHATFNMALWDRDFAAGAAAVDLDALISGIRARSAADVLALTRQPLRWRDLPN
                     PMALLPSQPSVNDCPLMTIAPGAPPTMRISNSFRRRLKGKERKLRTLPGYRYGVAVTD
                     AEIKRLLDWFFLVKPLRMAEQKLPNVFAEPGVEDFVRGACMAQVPSGGHVIDIHALEC
                     DDEVIAIFAGVADGHRFSIMFNAYTMSDNSRVSPGLILMRNIVDHHAERGYLALDLGI
                     GSDDYKRLFCKSDEPIFDCFIPLSPRGKMAAAAMSGINRAKHLVKHSPALFHMAQKLR
                     DALAR"
     gene            8552..9604
                     /locus_tag="VGA15_00690"
     CDS             8552..9604
                     /locus_tag="VGA15_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9586247.1"
                     /translation="MPSENPFLARAGLELAYLSGYSRLMQRRTGGAGVILRFECVRPR
                     RATRFQPLRSREITPRFLDRAIAALKRWRFDIVSVDEVCRRAVTLASPRRFVCLTFDG
                     GYKDMITSAYPVLAKHGVPFTIYVPTAFPDRLGEAWWLALEEVIARENRISLVMDRKE
                     QRFNVGDTSEKYQLYDFLAGWMRSLPPPDLSVAINDLCKRYSVNLAALSREAMMDWDD
                     LTKLAADPLVTMASATVNYPVLSNLRDVDALREMAMGRAVAEAAFRRDVRHFAYPFGD
                     RAAFGRQHVGIAEEAGFASAVSAISGVVQTEGRTNLQALPRIAWDGRQRSLRVMRVIL
                     SGVTFRQVKRGPDAKF"
     gene            complement(9754..9960)
                     /locus_tag="VGA15_00695"
     CDS             complement(9754..9960)
                     /locus_tag="VGA15_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEX9586248.1"
                     /translation="MTIRTRKFFGTIALLLLVVVWSLMGMTVAQTPWLANSGLLQAVF
                     YVVAGIGWVLPAMPIISWMARPDV"
     gene            10056..11147
                     /locus_tag="VGA15_00700"
     CDS             10056..11147
                     /locus_tag="VGA15_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX9586249.1"
                     /translation="MIGIPAQQASPTRAVRWWLFGVAALIAIMVLVGGATRLTESGLS
                     IVEWKPVTGTLPPLNQEQWTEAFDAYKTIPQYRELNAGMNLAEFKTIFWWEWSHRLLG
                     RVIGVGYLLPFLWFLWRGILSTDLRRRLWLIFGLGALQGAVGWWMVASGLSQRVEVSQ
                     IRLATHLVLALLIFACIVWTLRRLTDRPSSVASSRLKIAGVALVVLTFVQLYLGALVA
                     GLRAGKVYNTWPDIDGALIPSAARLFFEQPWWRNLFDNVLTVQFEHRMTAYALFALAV
                     LHALDAVRSRAAPAVVNGAFWLAAAITLQATLGILTLLNEVPASLALAHQAVAIAVLT
                     LAIFQAERLAARQPTPEQQNLAMPVGQPG"
     gene            complement(11181..11534)
                     /locus_tag="VGA15_00705"
     CDS             complement(11181..11534)
                     /locus_tag="VGA15_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9586250.1"
                     /translation="MSDSIDLSEKLATFSEHFSPRAVARFNNCDVMVVKLKGEFVWHK
                     HDDTDDFFLVLKGVLDIQLRDRTVTLGPGQMYIVPKGVEHRPAAKEEVHLLLIEPTGT
                     PNTGDVKTAAPRKTA"
     gene            complement(11564..>11907)
                     /locus_tag="VGA15_00710"
     CDS             complement(11564..>11907)
                     /locus_tag="VGA15_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586251.1"
                     /translation="GRQDHTTSPSTAASFVRTHFARLTPQRPSHPAPYVRDDREAPLL
                     WEQDGESFRDDLGQLKTKIFLQTGLDRLMGDLPVGQNYVQEPTESRREKCQQEIRSPA
                     VTGLPANDTVR"
BASE COUNT         2311 a         3651 c         3823 g         2122 t
ORIGIN      
        1 cgtgcccagg aacgccgcga agatcgcaag ataggcgcgt tgccagggca caggcacgaa
       61 gatatgccga aaccccatga tgacggtgat gaacaccagc gccggatagc agacgccggc
      121 gatccagccg ccggacatga aagcgttgag gtaggaattg tgggtgtctt cgggaaaata
      181 tttgtggaac tgcagcgggc cgataccgaa tggcaaatcg agcgccatcc ccgcgccgag
      241 gatgtgccgt ccgaaccggc cgaagcggcc ctcgtcgtag ctctgatcga agctcgcgcg
      301 ttgcttgaac atctcggcga ttgaatcgaa cgacaacagc accgcgatca gcgccgccac
      361 gataatcgcc gtgaccaccg tcatcatgat gatgcgcgaa cgctgcgcgc gcgaccggct
      421 ggtcaggacc atcagcgcca gcatgaagct tgaggtgatg gcgagcccgc cccaggccgc
      481 gcgcgaaaac gccagcagga tcgcgagcgc catgatgccg agcgcaatcg cgctgcggaa
      541 cgattttcga aaatcgtcgg agacgacgct ttgcagcgcg aacagcgcag gcaggatcag
      601 gaaggcggcg agcacgttcg ggtccttgaa agtgccgctg gcgcggtcat acagcgtcag
      661 caggtcatgt ccgccgggga cgaggttgaa gtagccggcg atgccggcgg tcgccgcgat
      721 caaggcgccc accgtcaggc cgcggctgat catgtcaagc cgggccgcgg tgtcttcgga
      781 cagcaccatt gcaaggaaga tcacggtgaa cgccatatag caggaggtcg cgatccagtt
      841 ggcgacctcg ggcttgtcca tcagcgaggc ggcgctgatg cagtagccga tattgaccag
      901 gaacagcagc agcaacagcg gcatgaatac cagccgcagc cgcaagccgg tcgcaaagaa
      961 gatgacggtg gcggcgagcg tcaccagctc gtaggggctg ggttcgatga aaacgatcgc
     1021 gccggatgcg ccgaccagcc acaccagcgc gcgctgcagc gccagaacgc ccggcgcagc
     1081 cgtcatcggc ggtagggagt cctcggctgt cgccacatac gccatcacac actcgccaac
     1141 ttacgcaacg caaccggtag cccaacgccc gaaccggctg tcttgccagc tgtcatgcca
     1201 gccgtcttgg gggcggtgct taataagcgt tgtcgttctt cgtcatcagc gccggcggcg
     1261 tcttgagaag aatgtagagg tcgaacagca ccgaccagtt ctcgatgtag tagagatcga
     1321 attcgacccg cttctggatc ttctcttcgc tgtcgacttc gccgcgccag ccattgatct
     1381 gcgcccagcc ggtaatgccg ggcttgacgc ggtgccgcgc gaaatagccg tccaccgctt
     1441 cgtcgaacag ccggctttgc aatttgccct gcacggcgtg cgggcgaggg ccgaccagcg
     1501 aaagattgct cttgaacacg acgttgaaga gctgcggcaa ttcgtcaagg ctggtcttgc
     1561 ggatgaagcg cccgacgcgg gtgacgcggc tgtcgttcct ggtcacgacc ttgacggcca
     1621 gcggatcggc ctgatcgtgg tacagcgagc gaaacttgaa gacgtcgatg cgctcgttgt
     1681 tgaagccgaa ccgcttctgg cggaacagca ccggcccggg actgtcgaat tttacggcga
     1741 gcgcgaccag cgccatcaga ggcgaggcca gcagcagaat gacggcgccg acgacatggt
     1801 cgaacagcca cttcatcacc agatcccagt cggtgatcgg cgcctcgaac acatcgaggg
     1861 tcgggacctt gcccaaatat gaataggagc gggggcggaa gcgcagcttg ttggtgtggg
     1921 cggacaggcg gatgtcgacc ggcagcaccc atagtttctt cagcatttcg agaatccgcg
     1981 tctccgccga aatcggcagc gcgaacagca cgaggtcgac ccgggtgcgg cgggcgagtt
     2041 cgacgatatc gtcgaccttg ccgagtttgg ggctgcccgc gcaggtgtcg agcacgcggc
     2101 tgtcgttacg gtcatcgaac acgccgagga cgtggatgtc ggaatcttcc tgggtcttga
     2161 gcgcctcgat cagcttctca ccggtctgat ccgagccgac cacgatggtg cggcggtcga
     2221 gccggccgtc gcgcgcccag ccgcgcacca gcgcgcgaag aaacagccgt tcggcgagga
     2281 gtgcggaaag gccgacaaag aaaaacgccg agagccagag ccgcgatacc tcgccgccga
     2341 gttttgcgaa gaacgatgcg ccgataaaca gcaggaacac gaatgcccat gacgagatca
     2401 ttcgcgtcat ctgccggagc tgtccgcgga aaacctgtac ttcgtagata tcggcggcct
     2461 ggaagcagag caccgcggtc gcggtcatgc ccaggatggc ggcgatgtat tcccaatgaa
     2521 agccgctgag aggaaccaca tagccgagat agagcgcgac gcccaccagg ctgaccagca
     2581 caaaatcggc gacgcggacc gcgcccgcaa tcacgatcgg cgaatacgcc ctgcgcactt
     2641 tctggttggc gacggcaagc gcggcatgcg acagccgctg gcgccgctcc gttggagggc
     2701 gaaccgccgt agccgctgcc gcggcatcta agatggagcg tgcgttgatc ggttccactt
     2761 atccacgtcc gttcttgcac aggcgccttg gcttgcgcgc accaaggcgg aatttcccgc
     2821 ctcacggctt agaggacaaa tcggaacaaa cagttgaaag agcaggtatc gcggttaaga
     2881 gcggttaacg ctttgcaaac gcctcgcggt aacccgccaa caccccctcg accatcgcct
     2941 tttgcgaaaa atgcagaaag attctttcgc gaagggattt tgcccgcgcc tgcgcggctt
     3001 cggggtcttc gagcgcgatc tcgatggcgt ccgccatcgc gctgacgata ttgggagcaa
     3061 acagcgcctc ggtgtgcgat ccgaagatct ccgggatgcc accgacattc gcggcgacca
     3121 tcggaattcc ggccgccgcc gcctcgatca ccacataggg catcgaatcc ccacgcgacg
     3181 gaaccaccag tacaccacct ttcgaaaaac cgtagcgcgc cttgacgtga ccgatgaagc
     3241 ggaccgcttc ggtcagaccg agcctttgga tttgagcctt cagcgcctcg agttcctcgc
     3301 catcgcccgc cagcgtcagg gtcaccggtt tgccgtcggc gtgcagccgc gccaccgcat
     3361 cgatcaagag atccgcgccc ttgatgtgcc ggaactcgcc gacatagacg acgtcggtgg
     3421 catcatccgc cttgacgacg gggtcgaact cctccgcggt caccccattg aatacgcagc
     3481 gcaccagccc cttgggcgtg ccgatggtgc gctgataggt atcgcgcgcg aaggtgcttt
     3541 cgaacaggaa cagatcggta gagtccgtca gcgcgcgttc gaggcggctg tagagcgcgc
     3601 ccttgatcgt attcaggcgg taatgcagcg agccgccatg cggcgtatag actcgaatgg
     3661 tggctttgga gccggttttc aggcggatga aggcgccggc tttggcgcca tgtccgtgca
     3721 ggacgtccgg cttcagccgc cggatcaggc gcatgaaacg cgcccatacc agcacgtcgg
     3781 tgggaaaagg ttcgcggcga atcgcgaggc gatgaacacc aagcgcgagg cgcggcgcga
     3841 tggccgccag cgcggcctcg gcgcgttcgc cgccggtcag gctgtcggcg ataaggccga
     3901 cctggtggcc acgatcgacc tgcccgttgg cgaggtcgag gatgtgccgg aagattccgc
     3961 cgaccggcgt gcgcacggcg tgcaggatac gaagcggctg atcggccatc atggacatga
     4021 tcagaaccag cgttcgccga cgaggatggt gtcgccgggg ccaagcgcgg tgccgagcgg
     4081 cactacaaag cgggatgaac cggaatggtc ggtgtgggtc agcgtgacgc ggtcgcgcag
     4141 ggcgcgcggc gaaaatccgc cggcgatggc caccgcgctt tcgacgctca tgttgggtat
     4201 gtagggatac tggccgggcg ctgcgacttc accgaggatg aagaacggcc ggtaggcctc
     4261 gatctccacc gccaccgacg gttcgcggat atagccgttg cgcagtttgg ctgatatcgc
     4321 cgaggcgagg cccgccggcg ttcgtccgcg cgccggcacc gcgccgatca gcggcatcgt
     4381 gatcgcgccg ccggcatcga tcgcatagct gttggtgaga ccttcctggc catagaccac
     4441 gacgcgcaat ttgtcgccgg cgtcgagccg atagaccgca tcgtggccag gcggcattgg
     4501 cgcggccgca taggcaaccg gcaccggagc gtcagcgcgc cgaggtgccg cggcaaagct
     4561 gctgcgaagc gcgctgatgg cgccgccgga atccgcgaca tcggatcgcg acgaaggttc
     4621 aagagccggg ttggtgggct ggccgtaggc caacgaatcc agatcgcttt gcggctgcag
     4681 ccctgcgaca ggcgcggaca tgcgcatgca gcccgacaga gcgagcgctg tgagcagaca
     4741 gagtgtaacg gcgtgcacgc ctcgcatcta ataaccctga ggaagtattt gctccgatta
     4801 agggctttca tggttaacaa agcatgagcg cggcgggcca agcctggatc aagcctcgat
     4861 gttggcgcgc gcaaatccgt gaaattaacg cagcggcaac cttaatggag tgtaatcgcc
     4921 aaggttgagc ggcgcgtagc gtctgcggga gtgtgcgatg cgtttaccgt tctggcgtgc
     4981 gggcaaagat aaggcggcca cggcagagcc ggtggtccag accgccatcc cgacgcccgc
     5041 gccggccacc gacaagccgg ccgtggcatc cgaatctggc gatctcgatt tgcgcgcgct
     5101 cggccaggcg ctggtgcgca acaggggctg ggtcatcgtt ccgaccgcgc ttgccgccgt
     5161 actctcgatt gccgccgtca atctggttac cccgcgatac aaatccgaag cccgcatcct
     5221 gatcgacgga cgcgagaaca ttttcctgcg gccgaatggc gaacgcagcg aggagcgcaa
     5281 cgcgctcgat gccgaggccg ttaccagtca agtgcagcta ttgctgtcgc gcgatcttgc
     5341 gcgcgacatc atcaagaaga acaagctcgc cgagcggccg gaattcgatc cggtgctgca
     5401 gggcatttcg ccggtaaaat cgctgctggc gttgttcggc atcggtcgcg atccgttctc
     5461 gttgacgccg gaagagcgcg tgctggatgc ttatttcgac cgcttcaccg cctacgccat
     5521 cgataaatca cgcgtcatcg tcatcgaatt ccagtcgcgc gatccggatc tggcggcgcg
     5581 ggtcgccaac tcgattgccg aaggctacct ggtgttgcag cagggcgcgc ggcgggagca
     5641 ggccaaatcg gccgggcagt ggctgtccgg cgaaatcgac aatctgcgca agaaggtcgc
     5701 ggaggcggaa tcccgcgtcg aggattttcg ctcgaagtcg agcctgtttg tcggcaccaa
     5761 caacaccacg ctctccaacc agcagatggg cgaactgaac acgcaattga acaacgcccg
     5821 cgcgctgaaa tccgacgcgg aatcgaaagc gcggctgatc cgcgagatgc ttcaaagcgg
     5881 caagccgatc gaggcttccg aggtgctcaa ttccgaactg acccgccgac tgtccgaaca
     5941 acgcgtcacg ctgcgcgccc agcttgccga gcagtcatcg acgctgctcg acaaccatcc
     6001 gcgcatcaag gagctgaggg cgcagcttgc ggatctcgac cgccagcttc gcgaggaggc
     6061 cagcaaaatc tcgcgctcgc tcgacaatga ttcacgcatc gccggcggca gggtcgatgg
     6121 gctcagcgcc agcctcgagc aattgaagaa gcaggcgacc tcgagcaata gccaggatgt
     6181 gcagcttcgg gcgctggagc gggaagccaa ggcgcagcgc gacctcttgg aatcttatct
     6241 ggcgaaatat cgcgaggcga ccacccgcga gaatatcgac gcggcaccgg cggacggcag
     6301 gatcatctcc agcgccagcg tttccaacac cccggcatac ccgaagaagc tgccgatcgt
     6361 gctgatcgcg acgctggcga cgttgatgct cagcacgggc gccatagcaa ccggagaact
     6421 tttgcgcatg acggcgcccc gcacgacagc ggcgttgacg tccccaccgg ctccagtgga
     6481 gcgcacgcca gcgccagcgg ctgcggctgt tgccgctgtc gccgccgagc ccgctccctt
     6541 gcccaaagag gcccaccccg acatcgcggc cgggcccgcc gagatcgagc ggctggcgga
     6601 cgacgtgcac aaagcgggcg acgcggcacg caaagtcacg atacttggta cggccgcaaa
     6661 cgagagcatc accctgacgg cgctgacgct ggcgcgcctg ctcgcgcgcg attcaagggt
     6721 cgtggtggtc ggtctgtcgg cgtcgtcgtc ggcaatctcg gcggcctcgg tcgatccgga
     6781 cgcaccgggt tttgcggaac tgatgctggg cgagacctcc ttctcgcacg tgatcaccaa
     6841 ggaccgctcg tcgcgcgttc atctggtgag cgccggacgt ccgggctcgg accgcgcgtt
     6901 actgcaatcg ccgcggctcg cgcttgcgat cgatgcattg ctgcgggtct atgatcacgt
     6961 gttgctggat gctggcactg cctccgacct gcctgcggaa ctgctgacag cgcaggcgcg
     7021 cgccgtggtg gtaccggatc cgtcgatgac cgcggacgcc cggacgtcga tgtgcgatca
     7081 gctgagagca gccggttgtt tcaccgggtt gacgatgctg agcgagccgg ttcagccctc
     7141 ggatgccgtg gagcccggcc cgcgggtcgt ggcggcctga acgtgatcat cgcctcgttt
     7201 tgagagaaac gccgcggtca gcgggcaagc gcgtcgcgca gcttttgcgc catgtgaaac
     7261 agcgccggac tatgcttcac cagatgcttg gcgcggttga tgcctgacat cgcggccgcc
     7321 gccattttgc cgcgcgggct gagcggaatg aagcagtcga aaatcggctc gtcgctcttg
     7381 cagaacagcc gcttgtagtc atccgatccg atcccgagat cgagcgcaag atagccgcgc
     7441 tcggcatggt gatcgacgat gttgcgcatc aggatcagcc cagggctgac ccgcgaatta
     7501 tcggacatgg tgtaggcatt gaacatgatc gaaaaccgat gcccgtcggc gacgccggcg
     7561 aagatcgcga tcacctcgtc gtcgcattca agtgcgtgga tatcgatgac gtggccgccg
     7621 ctcggaactt gcgccatgca ggcaccgcgg acaaaatcct cgacgccggg ttcggcgaac
     7681 acgttgggca atttctgctc cgccatccgc agcggcttga ccagaaagaa ccaatcgagc
     7741 agccgcttga tctcggcatc ggtgacggcc acgccatagc gatagcccgg cagcgtccgc
     7801 agcttgcgct ccttgccctt gagccggcgg cggaacgagt tgctgatccg catggtcggt
     7861 ggcgcgccgg gagcgattgt catcagcgga caatcgttga ccgagggctg gctcggcagc
     7921 agcgccatcg ggttaggcaa atcccgccag cgcaacggct gtcgggtcag cgccagcacg
     7981 tcggccgctg atcgcgcgcg tattcccgat atcagggcgt cgagatcgac agcggctgca
     8041 ccggcggcaa aatccctgtc ccagagcgcc atgttgaagg tggcgtgctt gccacccatg
     8101 aagcaagcgg tgcggacgcc gcactgatgg ctgagcgcga gcggaagcag caacaacgga
     8161 cgacgctcgg cgtcgtaggc gataacgatg aacggtgaaa gaccctcacg ctctccgacc
     8221 tggcgttgcc acggcccaag aaaatcgaac cgctggtacg gcgtggagaa ctgctgcgga
     8281 tcttcgagag cgcgccagat cgcctcggcc tgaacaagat cgtgaaggat atcgacgccg
     8341 gcaatgcggc ttgatttcga ccacgcgagc tcatccgccg ttcggctttc gatcgcggcg
     8401 gccatggtca ttgcaaaacc cgtctacagg aaattattta tatttttcgg cgtagccgac
     8461 ctttgcaaag aaaggtcaac aaagagtaag gacagggcgg ccgcggcggg acggaaatat
     8521 ccgcattaat ccgcattaag gggacgagac gttgccatct gaaaaccctt ttctggcacg
     8581 tgcggggctg gagctcgctt atctcagcgg ctactcgcgg ctgatgcagc ggcgcaccgg
     8641 cggcgccggt gtcattttgc ggttcgaatg cgtgcgtccg cggcgcgcca cgcgctttca
     8701 accgctccga tcccgcgaaa tcacgccgag atttctcgat cgagcgatcg ctgcgttgaa
     8761 gcgctggaga ttcgatatcg tttctgtgga cgaggtctgc cggcgggcgg tgacgctggc
     8821 ctcgccgcgg cggtttgtct gcctgacatt cgacggcggc tacaaggaca tgatcacatc
     8881 agcctacccg gtgctggcga aacacggcgt tcccttcacc atttatgtgc cgacggcgtt
     8941 tccggaccgt cttggcgagg catggtggct ggcgcttgaa gaagtgattg cgcgcgagaa
     9001 tcgcatcagc ctcgtgatgg atcgcaagga gcagcggttc aatgtcggcg atacgtcaga
     9061 aaaatatcag ctctacgatt tcctcgcggg ctggatgcgc tcgctgccgc cgccggatct
     9121 gtcagtcgcg atcaacgacc tctgcaagcg ctactccgtc aacctcgcag ccctgtcgcg
     9181 cgaagcaatg atggattggg atgatttgac aaaactcgcg gccgatccgc tggtgacgat
     9241 ggcgagcgcg acggtgaact atcctgtcct gtccaacctc agggatgtcg acgccttgcg
     9301 cgagatggcg atggggcggg cggtcgcgga agctgcattc cggcgggatg tccggcattt
     9361 cgcctatccg ttcggcgatc gcgctgcgtt tggccggcaa catgtcggga tcgccgagga
     9421 ggccggcttc gccagcgcgg tgtcggcgat atcgggcgtg gtccagaccg aaggccgaac
     9481 caatctgcag gcgcttccgc gcatcgcctg ggacggacga cagcgctcgc tgcgcgtcat
     9541 gcgtgtgatt ctttcgggcg tcacattccg tcaggtgaaa cgcggtccgg atgcgaaatt
     9601 ctagagcatg atccggaaaa gcatgccctc gggccacgac ccgatgggtg ggtaccggtt
     9661 ttccctcagg acaaacgcga agcgtttgcc cggagatcat gctcaaacaa aaagatagag
     9721 cgggatgacg attcgaagaa aagtcattcc gctctagaca tccggccgcg ccatccagct
     9781 gatgatcggc atcgcgggaa gcacccagcc aatgccggcc acgacataga agacggcctg
     9841 cagcagcccg gaattcgcca gccacggcgt ctgtgccacc gtcatcccca tcagcgacca
     9901 gaccacgacg agcaggagca gggcgatggt tccgaagaat ttgcgggtgc gtatggtcat
     9961 gacggaaatg tggtgcccgt tgcagctcgg cgcgccttgc gcgcgggtag gggcggacta
    10021 taaggggcga gcaaattcat tcaagcgcag cttcgatgat cggtataccc gcacaacagg
    10081 cgtcaccgac ccgcgccgta agatggtggc tgttcggcgt cgccgcgctg atcgcgatca
    10141 tggtgctggt cggcggcgcc acgcgcctga ccgaatccgg gctctcgatt gtcgagtgga
    10201 aaccggtcac cggcactctg ccgccgctca atcaggaaca atggacagag gcgttcgacg
    10261 cctacaagac tataccgcaa tatcgcgaac tcaacgccgg catgaatctg gcggagttca
    10321 agaccatctt ctggtgggaa tggagccacc ggttgctcgg gcgcgtgatc ggcgtcggtt
    10381 atttgctgcc gtttctctgg ttcctctggc gcggtatcct gagcacggac ttgcggcggc
    10441 ggctgtggct gatcttcggc cttggcgcgc tgcagggcgc ggtcggctgg tggatggtgg
    10501 cctccgggct gtcgcaacgg gtcgaggttt cgcaaattcg tctcgccacc catctggtgc
    10561 tggcgctgct gattttcgca tgcatcgtct ggacactgcg gcggctgacg gaccggccgt
    10621 catcggttgc atcttcgcgg ctgaagatcg ccggcgtggc tctcgtggtg ctgaccttcg
    10681 tgcagcttta tctgggcgca ttggtcgcgg gcttgcgcgc gggcaaggtc tacaatacct
    10741 ggccggatat cgacggtgcg ctgattccat ccgcggcgcg gctgttcttt gagcagccgt
    10801 ggtggcgcaa tctgttcgac aatgtcctca cggttcagtt cgagcatcgc atgaccgcgt
    10861 atgcgctgtt tgcgctggcg gttctgcatg cgctcgacgc ggttcgttcg cgggccgcgc
    10921 ccgccgtggt caacggcgca ttctggctgg cggcagcgat cacgttgcag gcgacgctcg
    10981 gcattctcac gctgttgaac gaggttccgg cgtcgctcgc gttggcgcac caggccgtcg
    11041 cgattgcggt gctcacgctg gcgatttttc aagccgagcg attggctgca cggcagccga
    11101 cgccggagca gcaaaacctc gctatgccag tcggccagcc cggctgaacc agcattttgc
    11161 ggaatcgcct ccaaaccgga tcacgccgtc ttgcgcggtg ccgccgtctt gacatcaccg
    11221 gtgttgggcg tcccggttgg ctcaatcaac agcaaatgca cctcttcctt cgcggcggga
    11281 cgatgctcaa cgccctttgg tacaatgtac atttggcccg gcccaagggt gaccgttcga
    11341 tctcgaagtt gaatgtcgag cacgcccttc aggacgagaa aaaagtcatc ggtgtcgtca
    11401 tgtttgtgcc aaacaaattc gcctttcaat ttcacgacca tgacatcgca attgttgaac
    11461 cgggcgaccg cacgcggcga aaagtgctca gaaaacgtcg ccagcttctc cgacaaatca
    11521 atactatcgc tcatggctcc ctccgacatc gtcgcttatg agtttaccgg acggtgtcgt
    11581 tagccggcaa tccagtaacc gctggagagc gtatctcttg ttggcacttt tcccgtcgcg
    11641 actccgtcgg ttcctgcacg taattttgcc cgacgggcaa atcacccatt agtctgtcca
    11701 gccccgtttg caaaaatatt tttgttttca gctgacccaa atcatctcta aaactctcgc
    11761 catcctgttc ccacaagagg ggcgcttcgc ggtcgtcacg gacgtaggga gcgggatgcg
    11821 atggacgctg tggcgtcagg cgcgcgaagt gcgtgcggac gaatgatgct gcagtggacg
    11881 gcgaagtcgt gtggtcctga cgccccg
//
LOCUS       DASWGM010000022        11577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1387198,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000022 DASWGM010000000
VERSION     DASWGM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11577
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387198"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VGA15_00715"
     CDS             <1..601
                     /locus_tag="VGA15_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX9586252.1"
                     /translation="VGEDQKQHLELSRDIAQKFNNDFSESIAAHGHGDRFFPQPEPLI
                     TGPATRGMSLRDGTKKMSKSDSSDNSRINLTDDADTIAQKIRKAKTDPEPLPSEEKGL
                     ETRPEADNLVGIYAALSGRSKAEVLGEFGGGQFSNFKNSLVELCVAKLAPIASEMKRL
                     VADPGHVDQVLVDGADRARAIAVETMAATKDIVGFVRQR"
     gene            791..1285
                     /locus_tag="VGA15_00720"
     CDS             791..1285
                     /locus_tag="VGA15_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9586253.1"
                     /translation="MTTQRRSYETGHKPKCLVIVDETAEWDRAVYYASRWAIRAGGGV
                     VMLRIIETEDQNQQWLGVADIMRAEAEEAANAALDRASGRANGIAAITPERVIREGDP
                     TEQLLDVIDKDVDIAILVLAANPGAEGPGPLITLMASQVGSFPIPVTIVPGDLSDADI
                     DALS"
     gene            1430..1999
                     /locus_tag="VGA15_00725"
     CDS             1430..1999
                     /locus_tag="VGA15_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX9586254.1"
                     /translation="MFIQTEATPNPATLKFIPGRAVLDSGTMEFANREAATRSPLAER
                     LFDVPGVTGVFYGSDFVTVTKDDGDWQHLKPAILGAIMEHYMSGAPLLADGSVGSDEA
                     ADEQDEFFNEADAETVATIKDLIETRVRPAVASDGGDITFRGFKDGIVYLNMKGSCAG
                     CPSSTATLQHGIQNLLKHFVPDVVEVRPV"
     gene            complement(2578..3585)
                     /locus_tag="VGA15_00730"
     CDS             complement(2578..3585)
                     /locus_tag="VGA15_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX9586255.1"
                     /translation="MAAALLLYPEISRADESGISFWLPGLEGSLAAVPTTPGWSLGTI
                     YYHTSVNATGAAAAAREFQVGRFSPTVNINLNLSLSGQADLLFVAPTYTFATPVLGGQ
                     LSATMAGVYGRNSASLAGTLTAAAGPIVTTRTGFLEDALVSYGDLYPTFKLKWNSGVN
                     NFMVYGAGDIPVGNYDPNRLANIGIGHGAIDFGGGYTYLNPVTGNEFSAVGGFTYNFK
                     NPDTQYQSGIDFHLDWGASHFLSKQVFVGLVGYAYQQITDDFGQPAVLGGFRSRVLGI
                     GPQLGYIFPIGDNQAFLGVKGYGEFDAANRPSGWNTWLTFAISEAAPKTVTPTKHLVT
                     K"
     gene            complement(4355..5359)
                     /locus_tag="VGA15_00735"
     CDS             complement(4355..5359)
                     /locus_tag="VGA15_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9586256.1"
                     /translation="MHQKAFASRKDDERWREMATHFSVQSRSLENFEELKRVVQSART
                     EITQVDRGRIQGNLSHLVIGDLPLDLGTFSLGVRSRGIVSEDRITIGMLTACNNRVTH
                     WSHEMLPADVVVWPPGAEHDARYYGGASVAVISLDASDIDLIFGSEPRLRDFGVWTKS
                     HYRPDPNAGADVIGNLHAIAAGLHASGIGLSAEAAEFWKRTIVDTIASTILENSPSDR
                     DGPIPSALRIVKKVEDYINTTGQKPVHISEICNRLHVSRRTLHRAFHNAIGIGPVSFL
                     CYKRLCSVNSALRSSDPATTTIGAVALQYGFINLGRFSGYYHSLFDEYPSQTLAARAD
                     "
     gene            5757..6146
                     /locus_tag="VGA15_00740"
     CDS             5757..6146
                     /locus_tag="VGA15_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586257.1"
                     /translation="MAVSPASALTGANSTLQQQFKNDNPVVEIVVRRGGAVRRTTVVG
                     PRGNVASRTVVRRGAVVRPGVVRPGGWVRPAHYYWRPGGAIAAGVAIGFVAAATAVAW
                     AGAAPAPGYCWYYTDPSRTQGFWDVCP"
     gene            6168..7157
                     /locus_tag="VGA15_00745"
     CDS             6168..7157
                     /locus_tag="VGA15_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX9586258.1"
                     /translation="MTLLPADIEQNDRSSEDSRTDDMVRIPGGTFRMGSDNHYPEEAP
                     AHRVTVDGFWIDRTPVTNRQFKSFVKATGHKTYAEIPPDPKDYPGALPHMIYAGSLVF
                     SPPQRVTNLRDWSQWWEFMKGADWRHPYGPKTNIDVHDNHPVVHVSFSDALAYAQWAG
                     KDLPSEAEWEFAARGGLDGTEFAWGGEFTPGGVHMANTWQGDFPRQNLNQDGFERTSP
                     VTAFPPNGYGVHDMIGNVWEWTSDWYTQKYDADAPKACCIPENPRGGREEGSYDPCQP
                     NIKIPRRVIKGGSHLCAPNYCRRYRPAARHAEAIDTSTSHVGFRCVIRNPAPK"
     gene            7272..8900
                     /locus_tag="VGA15_00750"
     CDS             7272..8900
                     /locus_tag="VGA15_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9586259.1"
                     /translation="MLLGTSTLVAAATLTSNAFAQAQKAAPAAPTPAPAAPSGRKPNI
                     LVIFGDDIGYWNVSAYNRGMMGYRTPNIDRIAKEGAIFTDLYAQQSCTAGRAAFITGQ
                     SCFRTGLLKVGLPGAKEGLSEKDPTLADLLKPQGYATGQFGKNHLGDRNEFLPTVHGF
                     DEFFGNLYHLNAEEEPERPDYPKNPEFRAKFGPRGVLKCVATDTDDPTEDPRFGRVGK
                     QKIEDTGPLTRKRMETVDEEFLGAAMNFIDRNTKANKPFFCWFNSTRMHVFTHLKKDS
                     EGKTGLGIEADGMVEHDGMVGQLLKQLDDLGIADNTIVLWTTDNGAEVWGWPDGGTTP
                     FRSEKNSNWEGGYRVPGAMRWPGVIKPGIEINDIVSHEDWVPTLVAAAGDTNVTGKLL
                     TGHQAAGKTFKVHLDGYDLRDLLAGTGPSKRKEYFYWTDDGNLAGLRYDRWKIVFLEQ
                     RAEGLKVWQDPLIPLRAPKLFDLRGDPFERADHVAGNYDTWYAERMFVLVPAQAFVAQ
                     HLATYQEFPPRQKPGSFSLDQVLEKLQEAGTSKN"
     gene            8933..9085
                     /locus_tag="VGA15_00755"
     CDS             8933..9085
                     /locus_tag="VGA15_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586260.1"
                     /translation="MFKAAKFLDTFVAYPPSQRAPSFSVDQIMEDVKAKIANVKTLQS
                     QTTGAR"
     gene            9130..9246
                     /locus_tag="VGA15_00760"
                     /pseudo
     CDS             9130..9246
                     /locus_tag="VGA15_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
     gene            9257..9502
                     /locus_tag="VGA15_00765"
     CDS             9257..9502
                     /locus_tag="VGA15_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HEX9586261.1"
                     /translation="MSGLDIFAWIVLLILVASAVAVFCIAGWLPGHIAKTRGHPQAQA
                     VKVAGWVTLICGFALWPVALIWAYVDVPAPHRGEPQP"
     gene            9499..10440
                     /locus_tag="VGA15_00770"
     CDS             9499..10440
                     /locus_tag="VGA15_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9586262.1"
                     /translation="MMIAIMSVYLVLLFALVRLGIVTFNLFWKASPFIVLLLLSFGLF
                     IPMGWGAPQGDALVVRNSVAIVPSVAGEVIDVPVEANAALRAGDVLFRIDPLPYEAQV
                     KAIEAQLKLSATRLAQMTQLFERDSGRGFDVEQRQSEVDQLKAQLEGAKWNLDKTIVR
                     APADGYVTNVALRKGARVANLPLAPVMAFIDTSDTLIGVEIAQNDARYITPGQPVELT
                     FKLRPGIIYTGKVESVLQAISTGQTQTSGLAVTPKGVQSVPFVVRVKLDDEDVAKQLP
                     AGSTGTAAIFTEAVKATHVIRKVLLRQLAILNYVNPF"
     gene            10982..>11577
                     /gene="tsaB"
                     /locus_tag="VGA15_00775"
     CDS             10982..>11577
                     /gene="tsaB"
                     /locus_tag="VGA15_00775"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX9586263.1"
                     /translation="MLILAIDTALDACAAAVLDIDAGRLIAQESQAMKRGHAEALMPL
                     IARVIKAAGIAFAALDRIAVTTGPGSFTGLRVGLSAARGIALAADKPVVGVTTLTAYA
                     APIVAENREQPVVCAIDARHDHVYFQVVSGDGSSLIRPRLAPIEAALGASRFGAPHLV
                     GNAAGILAGRWPADAPPPFNVDAQPAPDIAWVAWLGAAV"
BASE COUNT         2380 a         3531 c         3519 g         2147 t
ORIGIN      
        1 cgtgggcgag gaccagaagc agcacctcga actgtctcgc gacatcgcgc aaaaattcaa
       61 caacgacttt tccgaatcga tcgccgcgca cggccatggc gacagattct tccctcagcc
      121 ggagccgctg atcaccggcc ctgcgacgcg gggcatgtcg ctgcgcgacg gcaccaagaa
      181 gatgtcgaag tccgattcgt ccgacaattc gcggatcaat ctcaccgacg atgccgacac
      241 catcgcgcag aagatccgca aggcgaagac cgatccggag ccgctgccgt cggaggaaaa
      301 gggtcttgag acgcgccccg aggccgacaa tctggtcggg atctatgcgg cactttcggg
      361 ccgcagcaaa gccgaggtgc tcggcgaatt cggcggcggg cagttttcga atttcaagaa
      421 ctcactggtc gaactctgcg tcgcaaaact ggcgccgatc gcctccgaga tgaagcggct
      481 ggtggcagat cccggccatg tcgatcaggt cctggtcgac ggtgccgacc gtgcccgcgc
      541 catcgcggtg gaaaccatgg ccgcgacaaa ggacatcgtc ggtttcgtcc gccagcgtta
      601 gttccatggc tttgccataa ttgacgcagc gcactctgct cttggcgcgt attctgatcg
      661 caaaacggta cccacctccg gatcaaatcc gagggcatgc ttttgcggaa cacgcgctgg
      721 cgctgcggct tgtcgaggat ggccgcgccg tggcagcatg tggcctgctt ggcgcagccc
      781 caggacatgc atgaccaccc agcgacgaag ctacgagacg ggtcacaagc caaaatgtct
      841 cgttatcgtt gacgagaccg cggaatggga ccgcgcggtc tactatgcca gccgctgggc
      901 gatccgggcc ggcggcggtg tggtgatgct gagaatcatc gagaccgagg accagaacca
      961 gcaatggctc ggggtcgccg acatcatgcg cgccgaggcc gaggaggccg ccaatgccgc
     1021 gctcgaccgc gcttccggcc gcgccaatgg cattgccgcg attaccccgg agcgcgtgat
     1081 ccgggagggc gacccgaccg aacagctcct cgacgtcatc gacaaggacg tcgatatcgc
     1141 gatcctggtc ctggccgcca atccaggggc ggaaggcccc gggccgctca tcaccttgat
     1201 ggccagccag gtcggctcgt tcccgatacc ggtcaccatc gtgcccgggg acctcagcga
     1261 cgcggatatc gacgcgttat cgtagggaat agtcgccccg gcgagcgcgc taccgatggg
     1321 catgggagct atcccacccc ttgcccaccc cttgatcgtg catcggccgt cgccatctgc
     1381 tagggatcgc tcacgcgccg gccttgaacc ggcgacgccg gagaaaacca tgttcattca
     1441 gaccgaggcc acgcccaatc ccgccaccct gaaattcatc cccggccgcg ccgtgctcga
     1501 cagcggcacc atggaattcg ccaaccgcga ggccgcgacg cgctcgccgc tcgccgagcg
     1561 actgttcgac gtgcctggcg tcaccggcgt attttacggc tccgacttcg tcaccgtgac
     1621 caaggacgac ggcgactggc agcacctcaa gccggcaatc ctcggcgcca tcatggagca
     1681 ctacatgtcc ggcgcgccgc tcttggccga cggcagcgtt ggaagcgacg aggccgccga
     1741 cgagcaggac gagttcttca acgaggccga tgccgagacg gtcgcgacca tcaaggacct
     1801 gatcgaaacc agggtgcgcc ccgcggtcgc cagcgacggc ggcgacatca cctttcgcgg
     1861 cttcaaggac ggcatcgtct atctcaacat gaagggctcc tgcgccggct gcccctcatc
     1921 caccgcgacg ctgcagcacg gcatccagaa tttgctgaag catttcgtgc ccgacgtcgt
     1981 cgaagtccgg ccggtgtaaa gccgcgctgc tccacaacga gttgtcatac tccgccaccg
     2041 ggtctcgcct tcggcgagcc cgatgacagg ctccagcgga gtatccagta cgccgcagct
     2101 tttcgattcc atcactgaca cctcggaata ctggatcgcg cgctggagcc tgtcatcggg
     2161 cggcgcaacg cgccgacccg gtggcgggcg atgacggctg agagtgtggc acactcacat
     2221 tcatcagaca cacgttcgcg accttgcggc gatgcccggg cggaagcgcc aacgggtccg
     2281 cgcaaagcgt ggcccgatga caggctccgc gcgtcttcgc gctagatgtc cgggcatcca
     2341 cgtcttagcc gtggtgccgc gagaaagacg tgggcatagg cgagcggaag cgacgccgtc
     2401 cttcggacgg ctatgcccgg ccatgacgag aacagcttac ccggtctcga tttgcccacg
     2461 gcgccatccg atggcaaaaa ggctcgcaag aataaacgag agcagccaac aaggctgcgc
     2521 ggtcttggag ataacaagac ttcagctttt gatccgtaac gcatgttggc ccaaaggcta
     2581 tttcgtcacc aggtgctttg tcggtgtcac cgtcttcggc gcggcctccg agatcgcgaa
     2641 cgtcaaccag gtgttccagc ccgatggccg gttggcggcg tcgaattcgc catagccctt
     2701 cacgccgaga aatgcctggt tgtccccaat cgggaaaata tatccaagtt gcggcccgat
     2761 gccgagaacg cgcgagcgga agccgcccag gaccgccggt tgtccgaagt catcagtgat
     2821 ctgctggtag gcatagccga ccaagccgac gaaaacctgt ttcgagagga aatgcgacgc
     2881 gccccagtcg agatgaaaat cgataccgct ctggtattgc gtatccggat tcttgaaatt
     2941 gtaggtgaag ccgccgacgg cggagaattc atttcccgtc acgggattga gataagtgta
     3001 gccgccgccg aaatcgatgg cgccgtgacc gatgccgata ttggccaaac gattcggatc
     3061 gtagttgccg acgggaatgt caccggcgcc gtagaccatg aaattgttaa cgccgctgtt
     3121 ccatttcagt ttgaaggtcg gatataaatc tccataagag acgagggcat cttccaggaa
     3181 tcctgttcgc gtggtcacga tcggccccgc ggccgccgtt agcgtcccgg cgaggctcgc
     3241 ggagtttctg ccgtagacgc cggccatggt tgccgacaac tggccgccga gcaccggcgt
     3301 cgcgaaggtg taagtcgggg cgacgaagag tagatcggcc tgcccgctca aactcagatt
     3361 tagattgata ttgacggtcg gagagaatcg tccgacctgg aactctcgcg cggctgcggc
     3421 cgcccctgtc gcgttcacgc tggtgtggta gtagattgtg ccaagagacc agccgggcgt
     3481 cgtaggaact gcagcgaggc tgccttctag tccgggcagc cagaacgaaa ttccgctctc
     3541 gtcggcgcgg gagatttcag ggtaaagcag gagagccgca gccattgccg cgcagccaat
     3601 tcgtcggatt ctcgattcaa ttgatgccat tgcaaacctc cacgcggtat ttgcagtggc
     3661 ggctaccaaa aggggccgca agcgcgaagg ccgcaatttc tgacgagcta aacgaattgc
     3721 ccggtagtta cgccacttta gatagtaggt cacaggtcgc gccaaaaatc cgtggcagat
     3781 aggttacagc cgagaatatt tggtgatggc aagtttatgt caggagcgct tccacaatcg
     3841 tcaccggccg ggcacgtgcc cacctagatt cgcgatcaat ttgaggttga ctgagggaac
     3901 cgagaggaac agcctactca aagggatgtc caaataggga agaggtgctg accgaatacc
     3961 ctcgccgtgc acatcaatga cgtggcgggc aaacatgaaa gggctggaaa tgcaatcgac
     4021 aatgatgaat ctcgttcgtc aacgtggctg cgcatcaaag actttcaacg gcatggcctg
     4081 cgctgtcgca cttggtttgg ctggctttgc gataacggct tcgccggcat cggctctcac
     4141 tgccggcgcc aacagcgcac tccaatatca actcaagcaa cttagcccga tcatcgaagt
     4201 cgtggtgcgg cgcggaggcg ccgtgcgccg aaccacggtt gtcggcccgc gaggtaacgt
     4261 ggcgagccgc acggtggtgc gccggggcgc cgtcgtgcgt ccgggtgttg tgcgtcccgg
     4321 tggctgggta cgtccggcca attactattg gcgtttagtc agcgcgagcg gccaacgttt
     4381 gcgaaggata ttcatcgaaa agggagtgat aatagccaga aaatcgaccg aggttaatga
     4441 agccgtactg caaggcgaca gctcctatcg ttgtcgttgc cggatcactg ctgcgaagag
     4501 cagaattgac cgaacataat cgtttgtaac agaggaacga caccggacct atgccgattg
     4561 cattgtgaaa ggcccgatgc agcgttcggc gcgaaacgtg caaccggttg cagatttcag
     4621 aaatgtggac cggcttctgt cctgtcgtgt tgatgtaatc ctcgaccttc ttgacaatcc
     4681 gcaaagccga agggatgggc ccatcacggt cggaaggcga attctccagg atcgtcgagg
     4741 cgatcgtatc cacgatcgtg cgcttccaaa attcagcggc ttctgcgctc aagcctatgc
     4801 cggaggcgtg gaggcccgcg gcgatggcat ggaggttgcc gataacatcg gcaccagcgt
     4861 ttggatcagg tcgataatga cttttcgtcc agacgccaaa atctctcaat cgcggctctg
     4921 aaccaaagat caaatcgata tcggaagcgt ccagcgagat cacagcaact gacgcgccac
     4981 cataatatcg tgcgtcatgc tcagctcccg gcggccaaac gacgacgtcg gctggcagca
     5041 tctcatggga ccagtgcgtg acccggttat tgcatgcggt caacatgccg atcgttattc
     5101 gatcctcact cactattccc ctcgaccgca cgccgagtga aaaggtgcca aggtccagcg
     5161 gtaaatcacc tatgaccaaa tgggacagat tgccttgaat acggccgcgg tcgacctgcg
     5221 tgatctccgt gcgagcactc tgaacgacgc gcttcaactc ctcgaaattc tcgaggctgc
     5281 gtgactgtac tgaaaaatgc gtagccatct cgcgccagcg ttcgtcgtcc ttgcgacttg
     5341 cgaaagcctt ctggtgcact cgtcacaatt gcaatctacc cgccgtttcc ggaagaattt
     5401 aagctctttg tcgaacaaaa ctgcttggga tcctcgcaaa cgatttatct gctgcgatgc
     5461 tcagattggc acaaatcgta catcacgccc gcctaaaatt cgcgtagatt tgcgaaatca
     5521 atctaaagtt ggccttccaa aatcgtcaat gcgtttaagg acacaatcaa atagggaaga
     5581 agtgctgacc gaccgatacc atcgccgcgc aaatcaatga cgcggcgagg ttgcaatcat
     5641 gaaagggctt ggaaatgcaa tcgacaatga tggctcttgt tcgtcaatgt ggctgcgcac
     5701 caaagacatt caacagtgcg atctgccggg ccgtaattgg tttgactggc ttcgccatgg
     5761 cggtttcgcc ggcgtcggcc ctcaccggag cgaacagcac gcttcaacaa cagttcaaga
     5821 acgataaccc ggtcgtcgaa atcgtggtgc gacgcggagg cgccgtgcgg cgaaccaccg
     5881 ttgttggccc gcgcggtaac gtcgcgagcc ggaccgtggt gcgccggggc gctgtcgtgc
     5941 gtccgggcgt tgtgcgtccc ggcggctggg tacgtcctgc ccactattac tggcgcccgg
     6001 gcggcgcaat cgccgcgggt gttgcgatcg gattcgtcgc cgcagccacg gcggtggcct
     6061 gggcaggcgc ggcgccggca cccggttact gctggtacta caccgatcca agccggacgc
     6121 agggattctg ggacgtttgt ccgtaacgca gcgtcccgga gtagaccatg acattgctgc
     6181 cggccgacat cgagcagaac gatcgatcat cggaagatag ccggaccgac gatatggtcc
     6241 gaattcccgg cggcacgttc cgcatgggct ctgacaatca ttatccggag gaggcgccgg
     6301 cacaccgggt gacggtggac ggcttctgga tcgaccgcac gccggtgacg aaccggcagt
     6361 tcaagagctt cgtcaaagcg actgggcaca agacctatgc cgagattccg cccgacccaa
     6421 aggattatcc cggcgcgctt ccacacatga tctatgccgg atcgctggtg ttctcgccgc
     6481 cgcaacgcgt gacgaatttg cgcgactgga gtcagtggtg ggagttcatg aagggcgccg
     6541 actggcgcca tccctatggg ccgaagacca acatcgatgt gcacgataat cacccggtcg
     6601 tgcatgtttc gttttccgat gcgctcgcct atgcgcaatg ggcgggcaag gatctgccga
     6661 gcgaagccga atgggaattt gccgcgcgcg gcgggcttga cggaaccgag ttcgcctggg
     6721 gcggcgaatt cacgcccggt ggcgtgcaca tggccaacac ctggcagggt gactttccgc
     6781 ggcaaaacct caatcaggat ggctttgagc gcacctcgcc cgtgacggca tttccgccga
     6841 acggctacgg cgtgcacgac atgatcggca atgtctggga atggacgtcg gactggtaca
     6901 cgcaaaagta cgatgccgac gcgccaaaag cctgctgcat tcctgaaaat ccgcgcggcg
     6961 gacgggaaga gggcagctac gacccctgcc agcccaatat caaaattccg cgccgggtga
     7021 tcaaaggcgg ttcgcatctg tgcgcgccga attactgccg tcgttatcgt ccggcggcgc
     7081 gtcatgctga agccatcgat acatcgacca gccacgtcgg atttcggtgc gtgatcagaa
     7141 acccagcccc aaagtgactt tcgtctttca tctgaagaat ggagagtgca atgagcagcg
     7201 aagccaacaa ccaggaacaa tttgaggcgc cttcgaaaaa ggctgacgac cgcgccctga
     7261 gccgtcgaaa catgttgctt ggtacatcga cgttggtggc agcggcgacg ctgacttcca
     7321 acgcattcgc gcaagcccag aaagctgcac ccgccgcgcc caccccggcg ccagccgctc
     7381 cgtccggccg caaaccgaac atcctcgtca tctttggcga cgacatcggc tactggaacg
     7441 taagcgcgta caaccgcggc atgatgggct accgcacgcc aaacattgac cgcatcgcca
     7501 aggagggcgc gatcttcacc gacctctacg cacaacagtc ctgtacggcg gggcgtgccg
     7561 cgttcatcac ggggcagagc tgtttccgaa cggggttatt gaaggtcggc ctgccgggtg
     7621 ccaaggaagg gctatcggag aaggacccga cgcttgccga tttgctcaag ccgcagggct
     7681 atgccaccgg ccagttcggc aagaaccatc tcggcgatcg gaacgagttt ctacccactg
     7741 tccacggttt cgatgaattc ttcggcaatc tctatcacct gaacgccgag gaggagcccg
     7801 agcgcccgga ctaccccaag aaccccgagt tccgcgcgaa gttcggcccg cgcggcgtgc
     7861 tcaaatgcgt cgcgaccgac acagacgatc ccaccgagga cccgcgcttc ggcagggttg
     7921 gcaagcagaa gatcgaggac accggccccc tcaccaggaa gcggatggag acggtggacg
     7981 aggagttcct cggcgcggcg atgaatttca tcgaccgcaa taccaaggcc aataagccgt
     8041 tcttctgctg gttcaactcc acccggatgc acgtcttcac ccatttgaag aaggactccg
     8101 aaggcaagac cggcttgggc atcgaagccg acggcatggt cgagcatgac ggcatggtcg
     8161 gccagcttct caagcagctc gacgacctcg gcatcgccga caataccatc gtgctgtgga
     8221 caaccgacaa tggagccgag gtatggggat ggcccgacgg cggcactacg ccgttccgca
     8281 gcgaaaaaaa ctccaactgg gaggggggct atcgcgtccc cggcgcgatg cgctggccgg
     8341 gcgtcatcaa gccgggcatc gagatcaacg acatcgtctc gcacgaagac tgggtgccga
     8401 cattggtggc cgcggccggc gatacgaacg tcacgggaaa actcctgacc ggtcaccagg
     8461 cggcgggcaa gaccttcaag gttcatctcg acggctacga ccttcgcgat ctgctggccg
     8521 gcaccgggcc gagcaagcgt aaggagtatt tctactggac cgacgatggc aacctagccg
     8581 gcctgcgcta cgaccggtgg aagatcgtgt tcctggagca acgggccgaa ggcctcaagg
     8641 tctggcaaga cccactcatc ccattgcgcg cgcccaagct gttcgacctg cgcggcgatc
     8701 cgttcgagcg cgccgaccat gtggctggca actatgacac ctggtatgcg gagcggatgt
     8761 ttgtgctcgt gccggcacag gccttcgtgg cccagcacct ggccacgtac caggagttcc
     8821 cgccccggca gaagcccggc agcttctcgc tggatcaagt gttggaaaag cttcaggagg
     8881 ccggcaccag caagaactga tatcgggctg acggaaacgt tgggtccaag gcgtgttcaa
     8941 ggctgcgaaa ttcctcgaca ccttcgtcgc gtatccgccc agccagagag cgccgagttt
     9001 ctcggtcgat cagatcatgg aggacgtgaa ggcgaagatc gcgaacgtga aaacgctgca
     9061 gagccagacg acgggcgccc ggtaaatggt tggttgcatc gcgccggccg gtagcgatgc
     9121 cggtcggcgc ttcccaattg cctgccgacc atgccgggct ggaattacat ggtgcgcctt
     9181 tatcgcccca gacccgaaat cctgaacgga gaatggaaat tcccggaacc gcagcctgtg
     9241 acatgaggtg acggccatga gtggactcga tatcttcgcc tggatcgttc tcttaatcct
     9301 ggtcgcgagc gcggtcgcag tcttctgcat cgccggctgg cttccgggac acatcgccaa
     9361 aacccgcggc cacccgcagg cgcaagccgt gaaggtggcc ggctgggtca cgctgatctg
     9421 cggttttgcg ctgtggccgg tcgcgctgat ctgggcctac gtcgatgtgc ccgcaccgca
     9481 caggggggag ccgcagccat gatgatcgcg atcatgagcg tctatctggt gctgctgttc
     9541 gcgctggtgc ggctcggcat cgttaccttc aacctgttct ggaaggcgtc gccgttcatc
     9601 gtcctgttgc ttttgagttt cgggctgttc atcccgatgg gatggggtgc cccgcaaggg
     9661 gacgcgctgg tggtgcgaaa ttcggtagcg atcgttccga gcgttgcggg agaggtgatc
     9721 gacgtccctg tcgaggcgaa cgcggcgctg agggccggag acgtcctgtt ccgcatcgac
     9781 cccttgccct acgaggcgca ggtcaaggcg atcgaggcgc aactgaaact gtcggcaacg
     9841 cgccttgccc aaatgacgca attgttcgag cgcgattccg gccgcggctt cgacgtcgag
     9901 cagcggcaat ccgaagtcga tcaactcaag gcgcagctcg agggcgccaa gtggaatctg
     9961 gacaagacga tcgtgcgggc accggctgac ggctatgtga cgaacgtggc gctacgcaag
    10021 ggcgcccgcg tcgccaacct gccgctcgct cccgtcatgg ccttcattga cacgtccgac
    10081 accctcatcg gcgtggagat tgcgcagaac gatgctcgtt acatcacacc gggtcagccc
    10141 gtcgagctga ccttcaagct ccggccgggt atcatttaca ccggcaaggt cgagagcgtg
    10201 cttcaagcca tatcgacggg tcaaactcag acctccggac ttgcggtgac gccaaagggc
    10261 gtgcagtcgg tgccattcgt ggtccgcgtg aagctcgacg acgaagatgt tgcgaagcaa
    10321 ttgccggccg gcagcacagg aacggctgcg atctttaccg aggccgtcaa ggccacgcac
    10381 gtcatccgga aagtcctgct ccggcaattg gccatcctca actatgtcaa cccgttttga
    10441 ttcgccgacg agacttaccg gctgcatcct ttcctggatt tcagcgagcg agaaaattct
    10501 ggttggcggt aacgatcttc ccgtccatct cgcattcgat cacggcctgt gatttgctga
    10561 tcgcggttgc ctgtgcaagc gcaatcgccg aggctgaatt attccgccat tgcaatatct
    10621 gatgactttc tgatttcggg aaacattttc aaggaagctt ccgagttcaa tatcaattcg
    10681 cggagcgcgg cggctttccg tggcgacacc gaatatttca cgattcgatt ttcgcaattt
    10741 ttgcaaatga ttgatcaagt aacgccgcat tgcccgagat caactcaaca ttgcggatgc
    10801 aacgaaagtt acgcctgtgc cgcagctgtt ttttgcattg cctaatgacg catagcatgc
    10861 gaaagcacgc aatggatgtt gcgttgatcg cgatctcgtc gcgcgtcaac ccgcaaacgg
    10921 cgcttgcgaa taacctgtgg gcagcacgca aattttgcgg caagcgtggt agaaaggtat
    10981 catgctgatc ctcgccatcg acaccgcgct ggatgcctgc gccgcggccg tactcgatat
    11041 cgacgccggc agactgattg cgcaggaatc gcaggcgatg aagcgcggcc atgccgaggc
    11101 cctgatgccg ctgatcgcgc gcgtcatcaa ggcggccggc atcgcgttcg ccgctctcga
    11161 ccgcatcgcg gtgaccaccg gccccggcag ctttaccggg ctgcgcgtcg gactctccgc
    11221 cgcgcgcggc attgcgctcg ccgccgacaa gccggtggtc ggggtaacca cgctgaccgc
    11281 ctatgctgcg cccatcgtcg ccgagaaccg cgagcaaccg gtcgtctgcg ccatcgatgc
    11341 gcggcatgac catgtgtact ttcaggtggt gagcggcgat ggcagttcgc tgatccggcc
    11401 gcgcctcgcg ccgatcgaag cggcgctcgg cgcctcgcgg ttcggcgcgc cgcacctggt
    11461 tggcaacgcc gccgggattc tcgccggccg ctggccggcg gatgcgccgc cgcccttcaa
    11521 cgtcgatgcg caaccggcgc cggacatcgc ctgggtggcg tggctcgggg ccgcggt
//
LOCUS       DASWGM010000023         6239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_279261,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000023 DASWGM010000000
VERSION     DASWGM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279261"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /gene="hslV"
                     /locus_tag="VGA15_00780"
     CDS             complement(<1..506)
                     /gene="hslV"
                     /locus_tag="VGA15_00780"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX9586264.1"
                     /translation="MQASQSQSPVWHGTTILTVRKGGKVVIGGDGQVSIGQTVIKSNA
                     KKVRKLGKGDVIGGFAGATADAFTLFERLESKLEQYPGQLTRAAVELAKDWRTDRYLR
                     RLEAMMIVADKDVSLVLTGTGDVLEPEAGVMAIGSGGNYALAAARALVDSDKDAETIV
                     RRALDIAA"
     gene            734..1327
                     /gene="hisB"
                     /locus_tag="VGA15_00785"
     CDS             734..1327
                     /gene="hisB"
                     /locus_tag="VGA15_00785"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX9586265.1"
                     /translation="MRKATIKRKTKETDIEVTVNLDGSGVSDVATGIGFFDHMLDLLA
                     RHSRIDMSVKAVGDLHIDHHHTTEDVGIALGQAVKQALGTMAGITRYASIHMPMDETL
                     SRVVIDISGRPILVFKVEFPRDKVGTFDTELVREWFNAFAINAGVTLHVETLYGENSH
                     HIAESCFKGLARALRAAVAIDPRAAGEVPSTKGQLGG"
     gene            1354..1884
                     /locus_tag="VGA15_00790"
     CDS             1354..1884
                     /locus_tag="VGA15_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEX9586266.1"
                     /translation="MPVYTVHAPVMNGDGVRATDRFAFVRDGFHFWAALLAPIWLAWH
                     RLWLALIGWIVVVVALDVGMAALGVGSAAVFGVNLLIALLTGFEAASLWRWTLSRRNW
                     RQLDVVVADDEEAAERRFFDRWTAKQRALSNDQSAVDRGGPPPTRDIPGQPFSKPPPL
                     PGSSIIGLFPEPGASR"
     gene            1881..2531
                     /gene="hisH"
                     /locus_tag="VGA15_00795"
     CDS             1881..2531
                     /gene="hisH"
                     /locus_tag="VGA15_00795"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX9586267.1"
                     /translation="MSVAIIDYGSGNLHSAAKAFERASRSMDVPEKIVVTRDPEVAFR
                     ADRIVLPGVGAFADCRRGLDALDGMIEAMTEAVRNKARPFFGICVGMQLMATRGKEHV
                     TTDGFNWIAGDVEKISPRQENLKIPHMGWNTLDLIREHPVLERLPLGPKGRHGYFVHS
                     YHLNASNEADVLARADYGGPVTAIVGKDTAIGTQFHPEKSQRFGLALISNFLKWKP"
     gene            2743..3486
                     /gene="hisA"
                     /locus_tag="VGA15_00800"
     CDS             2743..3486
                     /gene="hisA"
                     /locus_tag="VGA15_00800"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX9586268.1"
                     /translation="MILFPAIDLKNGQCVRLEQGDMARATVFNLDPAAQARSFAAQGF
                     EYLHVVDLDGAFAGKPMNAHAVEAMLRTVTMPVQLGGGIRDLQTVEAWLAKGVARVII
                     GTAAVRDPELVKGAAKKFPGRVAVGLDARDGKVAVEGWAETSEVTALEIARRFEDAGV
                     AAIIFTDIERDGLLKGLNLDATIELAERISIPVIASGGFASIEDVKALLTPRAKKLAG
                     AIAGRALYDGRLDASAALALIRNARVESA"
     gene            3859..4146
                     /locus_tag="VGA15_00805"
     CDS             3859..4146
                     /locus_tag="VGA15_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586269.1"
                     /translation="MKTFLLVFEDGSNPEIDLRSYVDSLDSGAEMYALDGHVCFLKSD
                     LSVSDLSGRLLRFSGSSLFFIVDVTSSEYEGRMLGVFWDFLKPRHLESAAE"
     gene            4146..4922
                     /gene="hisF"
                     /locus_tag="VGA15_00810"
     CDS             4146..4922
                     /gene="hisF"
                     /locus_tag="VGA15_00810"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX9586270.1"
                     /translation="MFKVRVIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGA
                     DELCFLDITATHENRGTMLDVVRRTAEACFMPLTVGGGVRTVDDIRVLLRSGADKVSI
                     NSAAVSRREFVKEASEKFGDQCIVVAIDAKRVKRGGGGERWEIFTHGGRNSTGIDAIE
                     YAQEVVSLGAGEILLTSMDRDGTRQGFDIPLTQAVADSIPVPVIASGGVGHLDHLVDG
                     IREGHATAVLAASIFHFGEFTIREAKDHMVRAGLPMRLDP"
     gene            5002..5325
                     /locus_tag="VGA15_00815"
     CDS             5002..5325
                     /locus_tag="VGA15_00815"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX9586271.1"
                     /translation="MPRFTIHDLAATIDVRAASGGETSYTRKLLDKGSEHCAKKFGEE
                     AVETVIAAVENDRDHLIAESADLLFHLLVLLKSRGVTLGDVEAELARRTTMSGLEEKA
                     ARKRD"
     gene            5340..>6239
                     /gene="coaA"
                     /locus_tag="VGA15_00820"
     CDS             5340..>6239
                     /gene="coaA"
                     /locus_tag="VGA15_00820"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX9586272.1"
                     /translation="MDIRAPEQQYNPYRIFSREQWARLRDDTPMTLEPGEFERLRSMH
                     DRLDLQEVEDIYLPLSRLLSIYVDAMLRLYQAQRQFLGIRDRKVPYIIGVAGSVAVGK
                     STTARVLQALLARWSPRPKVDLVTTDGFLFPNAVLERQGLMQKKGFPESYDLPTLLAF
                     LSDIKAGRRPVRAPVYSHLTYDIIPNQWTEVGRPDILIVEGVNVLQTGRLPRDGKAVP
                     VVSDFFDFSVYIDADESVLREWYVRRFLALRDTAFHDPRSYFHRYAPLSDEEATATAL
                     AIWERTNLANLEENILPTRPRATL"
BASE COUNT         1127 a         1862 c         1995 g         1255 t
ORIGIN      
        1 tcggcggcga tatccagcgc ccggcgcacg atggtctcgg catccttgtc ggaatcgacc
       61 aaagcgcggg cggccgccaa cgcataattg ccgccggacc cgatcgccat cacgccggcc
      121 tccggctcca gcacgtcgcc ggtgccggtc agcaccagcg acacgtcctt gtcggcgacg
      181 atcatcatcg cctccagccg gcgcaggtag cggtcggtac gccagtcctt ggcgagctcg
      241 accgcggcgc gggtcaattg ccccggatat tgctcaagct tgctttccag ccgttcgaac
      301 aaggtgaaag catccgccgt ggcgccggca aacccgccga tgacgtcgcc cttgccgagt
      361 ttgcggacct ttttggcgtt ggatttgatg acggtctggc cgatcgacac ctgcccgtcg
      421 ccgccgatca ccaccttgcc gcccttgcgg accgtcaaaa tggtggtgcc gtgccagacc
      481 ggcgactggg attgtgatgc ttgcatgaag acctcgcgtt ggcaggggat ttaggcattg
      541 gcgcgctcgg ttgcaattgg cgcgatttgg tccccggatt ccggtcacca taggccgaag
      601 ttcggcttcc aaggcgccat cccgcagtag cgaaggcgac atcggcctgt taaaagggcc
      661 gcgttttagg ccaaggattg gccaaggatc ggccaagagt cggtcaagcg tcggtcaagg
      721 gaaagcgggt ttcatgcgca aggcgaccat caagcgcaag accaaggaga ccgatatcga
      781 ggtgacggtg aacctcgacg ggtcaggcgt gtccgacgtt gcgaccggca tcggcttttt
      841 cgaccatatg ctcgatctcc tggcccggca ttcgcgcatc gacatgtcgg tgaaggcggt
      901 cggtgacctt cacatcgacc accaccacac cacagaggat gtcggtatcg cgctcggtca
      961 ggccgtcaag caggcgctcg gcaccatggc cggcatcacc cgctatgcca gtatccacat
     1021 gccgatggac gagacgctgt cgcgggtcgt gatcgacatt tcgggacggc caatcctggt
     1081 gttcaaggtc gagttcccgc gtgacaaggt cggtacattc gacaccgaac tggttcgcga
     1141 gtggttcaat gcctttgcga tcaatgccgg cgtgactttg catgtcgaga ccctatatgg
     1201 cgagaacagc catcatatcg ccgaatcctg cttcaagggt ctggcgagag cgctgcgcgc
     1261 ggcggtcgcg atcgatccgc gggcggcggg cgaggtgccg tccaccaaag gtcagctcgg
     1321 cggctgattt cctgttcgcg ggcgagaggt acgatgccgg tttacacagt tcatgctccc
     1381 gtgatgaacg gcgatggtgt ccgcgcgacc gacaggttcg cattcgtccg cgacggcttt
     1441 catttctggg ccgcgctgct agccccgatc tggctcgcct ggcatcggct gtggttggcc
     1501 ctgatcggct ggattgtcgt ggtggtcgca ctcgatgtcg gaatggcagc ccttggtgtc
     1561 ggcagcgcag cggtctttgg ggtcaacctg ctgatcgcgc tgctgaccgg atttgaagcc
     1621 gcgagccttt ggcgctggac cttgtcgcgg cgcaactggc gccagctcga cgtcgtggtg
     1681 gccgacgacg aggaggcggc cgagcggcgg tttttcgacc gctggaccgc caaacaacgt
     1741 gcgctgagca acgatcaatc ggcggtcgat cgcggcggcc cgccgccgac gcgggatatt
     1801 cccggccagc cattttcgaa gccgcccccg ctgccgggga gcagcatcat cggcttattt
     1861 ccggaaccag gagcatcgcg gtgagtgtcg ccatcatcga ttacggatcc ggcaatctgc
     1921 attccgccgc gaaagcgttc gagcgcgcgt cgcgcagcat ggacgttccg gaaaagatcg
     1981 tggtcacccg cgatcccgaa gtggcgtttc gcgccgatcg tatcgtgctg cccggcgtcg
     2041 gcgccttcgc cgattgccgc cggggtctcg atgcgctcga cggcatgatc gaggccatga
     2101 ccgaagccgt gcggaacaag gcgcggccgt tcttcggcat ctgcgtcggc atgcagttga
     2161 tggcgacgcg cggcaaggag catgtcacca ccgacggctt caactggatt gccggcgacg
     2221 tcgagaagat ttcgccgcgc caggagaatt tgaaaattcc ccatatgggc tggaacacgc
     2281 tcgacctgat ccgcgagcat ccggtgctcg agcgtctgcc gctggggccg aagggtcgcc
     2341 atggctattt cgtgcactcc tatcacctca acgcgtcaaa cgaggcggac gtgctggcgc
     2401 gcgccgatta cggcggtccg gtgacggcga tcgtcggcaa ggacaccgcg atcggcacgc
     2461 aatttcaccc ggagaagagc cagcgcttcg ggctggctct gatttcgaac tttttgaaat
     2521 ggaagccgtg acaaggtacc cccctcccca accctccccc gcaaggcagg gctatcgcat
     2581 atggaatttt cctatgcttg gcgtttcgag gggtcgtccc tgcgttcgca gggacgactc
     2641 gccgggagct tgtgcgaggt cacgatcgca tcaacacgcg gctcaattca gtcctatgcg
     2701 attcccctcc cccgcaaggg gggagggagc aggcaacatt ccatgatcct gtttcccgcc
     2761 atcgacctga agaacggcca gtgcgtgcgc ctcgaacagg gcgacatggc gcgcgcgacc
     2821 gtgttcaacc tcgacccggc ggcgcaggcg cgcagctttg ctgcgcaagg gttcgaatat
     2881 ctgcacgtgg ttgaccttga cggcgccttt gccggcaagc cgatgaacgc gcatgccgtc
     2941 gaggcgatgc ttaggacggt caccatgccg gtgcagctcg gcggcggcat ccgcgatttg
     3001 cagaccgtcg aagcctggtt ggcgaagggc gttgcgcgcg tcatcatcgg caccgcggcg
     3061 gtgcgcgacc ccgagcttgt gaagggagcg gcaaaaaaat tccccggccg ggttgcggtg
     3121 ggcctcgacg cgcgcgacgg caaggtcgcg gtcgaaggct gggccgagac ctcagaggtg
     3181 accgcgctcg aaatcgcgcg gcgtttcgag gatgccggcg ttgccgccat catttttacg
     3241 gacattgagc gcgacgggct gctgaaaggt ctcaatctcg atgccacgat cgagcttgcc
     3301 gagcgcatct cgattcccgt tatcgcgtcg ggtggctttg cttcgatcga ggatgtaaag
     3361 gcgctgctga cgccgcgtgc gaaaaaactg gccggcgcga tcgccggccg cgcgctctac
     3421 gatggccggc tcgatgcttc ggcggcattg gcgctgatcc gtaacgcgcg cgttgagagc
     3481 gcttgatgaa gctggaggaa ataattcgaa cgggcttatc ccgcgtagat actattgcgg
     3541 tcaagcggac cgccataaat cccgctctat ggctggtcgg actaattact cccctgtcgc
     3601 tcgttttgac cgctataatt ggtcgcccgg ttgatactgt tttgcttcgc tgcggccccc
     3661 agttcttttt acgttcgcag tctatgtggc gtttatgttt cgcgacccgg atcgactgca
     3721 gtctgaggaa tacagggtca ggcaacaagc gcaacgatac ctttacgaaa agggcgggag
     3781 taccgaaata gtcgatatag caaaccaagt ggctcggatc gaaacctcga aatctcggct
     3841 ggatggtgga gagaaagaat gaaaactttt cttctggttt tcgaagacgg gagcaatcct
     3901 gagattgacc tgcgtagcta tgttgattcg ctcgacagtg gagcggaaat gtacgccctt
     3961 gatggtcacg tttgcttttt gaaaagtgat ctttctgtat ctgatttgtc tggcaggtta
     4021 ttgaggtttt ccggttccag tctgtttttc attgtggatg tcacttcatc cgaatacgag
     4081 ggccgaatgc tgggcgtgtt ctgggatttt ctcaaaccac gtcacctcga atctgcggcg
     4141 gagtgatgtt caaggtccgc gtgatcccct gtctcgacgt caaggacggc cgggtcgtca
     4201 agggcgtcaa ctttgtcgat ctgcgcgacg ccggcgatcc ggtggaagcc gccatcgcct
     4261 atgacgccgc cggcgccgat gagctgtgct ttctcgacat tacggccacc catgaaaatc
     4321 gcggcaccat gctggatgtg gtccggcgca ccgcggaagc ctgcttcatg ccgctgacgg
     4381 tcggcggcgg tgtgcgcacc gtcgacgata ttcgcgtgct gctgcgatcc ggcgccgaca
     4441 aggtgtcgat caacagcgcc gccgtcagcc gccgcgaatt cgtcaaggaa gcgtccgaaa
     4501 aattcggcga tcagtgcatc gtggtcgcga tcgacgccaa gcgcgtcaag cgcggcggcg
     4561 gcggcgagcg ctgggaaatc tttacccatg gcggccgtaa ttccacaggc atcgacgcga
     4621 tcgaatatgc ccaggaagtg gtctcgctcg gcgccggtga aatcctgctg acgtccatgg
     4681 accgggacgg aacccggcag ggtttcgaca ttccgctgac ccaggccgta gcggatagca
     4741 tcccggtgcc ggtgatcgcg tccggtgggg tgggccacct cgatcacctc gtggatggca
     4801 tccgcgaggg gcatgcgacc gcggtgttgg cggcgtcgat tttccacttc ggggaattta
     4861 ccatacgtga agccaaggac catatggtgc gggccgggct gccgatgcgg ctcgatccct
     4921 gacgactctt tgtgacggaa gtgctacatc ccatgagatg agtggcgtcc cacctgcggg
     4981 cgcgcgtgcc gggttgagtt gatgccgcgt ttcacgatcc atgacctggc cgccaccatc
     5041 gatgtccgcg cggcttcggg cggcgagacg tcgtataccc gcaaattgct tgacaagggt
     5101 tcggagcatt gcgccaagaa gtttggcgag gaagcggtgg agaccgtgat cgccgcggtc
     5161 gagaatgatc gcgaccatct cattgccgag agcgcggact tgctgtttca cctgctggtg
     5221 cttctgaaat cgcgcggcgt gacgcttggc gatgtcgagg cggaactggc gcggcgcacg
     5281 accatgtccg gactggaaga gaaggccgcg cgaaagcggg actaataaag ggtggcttca
     5341 tggatattcg ggcgcctgag caacagtaca atccctaccg gattttctcg cgcgaacagt
     5401 gggcgcggct gcgcgacgat acgccgatga cgctggaacc aggcgagttc gagcgattgc
     5461 gttcgatgca cgaccgcctc gatctgcaag aagtcgaaga catctatctg ccgctgtcgc
     5521 gtctgttgtc gatctatgtc gatgccatgc tccgcctcta tcaggcgcag cgccaattcc
     5581 tcggcatccg ggatcgcaaa gtgccctaca tcatcggcgt cgcaggctcc gtcgcagttg
     5641 gcaaatcgac caccgcgcgc gtgctgcagg cgctgctggc gcgatggtcg ccgcggccca
     5701 aggtcgatct ggtgacgacc gacggtttcc tgtttcccaa tgcggtgctc gagcggcaag
     5761 gcctgatgca gaaaaagggc tttccggaga gctacgatct gccgacgctg ctggcgttct
     5821 tgagcgacat caaggcgggc aggcggccgg tgcgcgcgcc ggtctattcg catttgacct
     5881 acgacattat cccgaaccaa tggaccgagg tcggccggcc ggatatcctg attgtcgagg
     5941 gcgtcaacgt cctgcagacc ggacggctgc cacgcgacgg caaggccgtg cccgtggttt
     6001 cggatttctt cgatttctcc gtctatatcg atgctgacga atccgtgctg cgcgagtggt
     6061 acgtccgacg cttcctggcg cttcgggaca ccgcgtttca cgatccgcga tcgtattttc
     6121 atcgctacgc gccgctgtcg gacgaggaag ccaccgcaac cgcattggcg atctgggagc
     6181 ggaccaacct tgccaatctc gaagagaata ttttgccgac ccggccgcgt gcgacgctg
//
LOCUS       DASWGM010000024         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1110039,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000024 DASWGM010000000
VERSION     DASWGM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110039"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VGA15_00825"
     CDS             <1..172
                     /locus_tag="VGA15_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9586273.1"
                     /translation="AKQEIRGPDGGTVKFEIDPFRKHCLLNGLDDIGLTMEKKASIDA
                     YEAKAKNARAWA"
     gene            203..991
                     /locus_tag="VGA15_00830"
     CDS             203..991
                     /locus_tag="VGA15_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586274.1"
                     /translation="MRPDVVTFWHGPLDRLRQTCLRSQVAAGHNVTVYSFEPLAGLPD
                     GVGNAEAEAVLSHGFAERLRPTGPDGVWRDWTTLQFSDFFRMRLMAQRAGLWLDADVL
                     LLKPVEIDPAKPYFAWERPRQLGNSVLYLPSGDPIVAAFAALMRQNELTPDWLALRHR
                     LIFALRRLRGLRRLSDVRVAIYGPAALTALARRAGELHNALPKKSFYAVHAEPRLFFE
                     PADFSVLIGDPEIIGLHISPKGRGNQPPAAGSLYAWAAERFGTP"
     gene            1290..1703
                     /locus_tag="VGA15_00835"
     CDS             1290..1703
                     /locus_tag="VGA15_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586275.1"
                     /translation="MTAQEHPYPRVEMLIDIFGDWLKRRRELSEIRQMDRADFDRIAS
                     ELRVSPGELDTLVRQGPHAADELPTLLKALGIDQTDLARTEPMVLRDMERVCALCHDK
                     RQCDRDLAAGASAERYEGYCLNATTINALARQPGK"
     gene            1652..2203
                     /locus_tag="VGA15_00840"
     CDS             1652..2203
                     /locus_tag="VGA15_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586276.1"
                     /translation="MPERDHDQRFGPTARQISPLRQALSHSESMKISRAAWFHVFLSV
                     SLALAALFLIRLHYAGGAALSSDSASDGHRLAEAWCKDCHAIEATTAGTTGAANAAPA
                     FTAIANQPSTTELSLKVFLRSSHRSMPNLLITPDQADGLANYILSLKRNLFCLMLPHR
                     RDSSSSPLPLWERVAAMRSIADG"
     gene            complement(2366..3244)
                     /locus_tag="VGA15_00845"
     CDS             complement(2366..3244)
                     /locus_tag="VGA15_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX9586277.1"
                     /translation="MIRPRRSLLFMPGSNARALEKARNLPADGIIVDLEDSVAPEAKA
                     AAREQIAQAIAAGGFGKREVLIRVNALDSPWWIDDVTMAGKARPDGILVPKISSVKDL
                     TTIADRLGDMNADSSIRVWAMIETARAVLHAEELAASSCDSELRLAGFVFGPNDISRE
                     TRIRMRPGRAAMIPMITHCILATRAHGLEILDGPYSDFSNVDGFGQECAQARDLGFDG
                     KTLIHPGQIEACNAIFTPPAEEVAHARKIIVAFERPENASRGAIQLDGQMVERLHADM
                     ARRTIAIADAIAAIGH"
     gene            3345..>3805
                     /locus_tag="VGA15_00850"
     CDS             3345..>3805
                     /locus_tag="VGA15_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX9586278.1"
                     /translation="MSSFPEHLLEGYRAFTSQRLPTEQSRYRELSEFGQSPEVMVIGC
                     CDSRVSPEVIFDAGPGELFVVRNVANLVPVYAPDGGAHGVSAALEYAVRALHVKHIVV
                     LGHAQCGGIRAFVDNVAPLTPGDFIGRWMSMFIKPGEKVGQRDHETMADFTT"
BASE COUNT          671 a         1244 c         1188 g          702 t
ORIGIN      
        1 cgccaagcag gagatccgcg gccccgacgg cggcacggtg aagttcgaaa tcgatccatt
       61 ccgcaagcac tgcctgttga acggcctcga cgacatcggc ctcaccatgg agaagaaggc
      121 ctcgatcgac gcctatgagg cgaaggcgaa gaacgcgcgc gcctgggcct gatctcaacg
      181 tctaccaggc gaggcgacgt ggatgcggcc ggacgtcgtc accttctggc atggcccgct
      241 cgaccggctc cggcagacct gcctgagatc gcaggtcgcg gcaggtcata acgtcaccgt
      301 ctacagtttc gagccgctcg cgggattgcc cgacggcgtc ggcaacgccg aggccgaagc
      361 ggtgctgtcg catggttttg ccgaaagact gcgcccgacc ggccccgatg gggtatggcg
      421 cgactggacc accttgcaat tcagcgattt tttccgaatg cggctgatgg cgcagcgcgc
      481 aggcctgtgg ctcgacgccg atgtgctgct gctcaagccg gtcgaaatcg atcccgcaaa
      541 accctatttt gcctgggagc ggccgcgtca gctcggcaat tcggtgctgt atctgccatc
      601 cggcgatccc atcgtggcgg cgttcgccgc gctgatgcga cagaacgaat tgacaccgga
      661 ctggctggcg ctgcggcacc ggctgatctt cgcccttcgc aggttgcgcg gtttgaggcg
      721 gctctcggat gttcgtgtcg cgatctatgg tccggcggcg ctgaccgcgc tcgcccgccg
      781 cgccggcgaa ctgcacaacg ccctgccgaa gaaatcgttt tatgcggttc atgccgaacc
      841 aagactgttc ttcgagcccg cggatttttc agtgctgatc ggcgaccccg aaattatcgg
      901 gcttcacatt tccccgaaag gccgcggcaa ccagccgccc gcggccggaa gcctgtatgc
      961 ctgggccgcg gagcgatttg gtacgcccta aggcgtcgcg gcggttcttc ccctctcccc
     1021 ttgtgggaga gggtggctcg catcgccaga tgcgagacgg gtgaggggtc acgacctctc
     1081 gataggccga accccctcat ccggcacgga ctttcgcata gccggtgcaa ggcatcggcg
     1141 tcccttttca agaacggcgg ccgaaggccg cctatggcca ccttctccca caaggggaga
     1201 agggacagta gcccccgcgc ctgtttgatc cagcgcaacg acacttgggt cgattcgcgc
     1261 tctgatggga caaattcgga aggtgtccca tgaccgccca agaacatccc tatccccgcg
     1321 tcgaaatgct gatcgacata ttcggcgact ggctgaaacg ccgccgggag ctgagcgaaa
     1381 tccgccagat ggaccgcgcc gattttgacc ggatcgcgag cgagctgcgg gtttctcccg
     1441 gtgaactgga cacgctggtc cgtcagggac cgcatgccgc ggacgagctg ccaacgctgc
     1501 tcaaggctct cggcatcgac caaaccgatt tggcgcgcac cgagccaatg gtgctgcgcg
     1561 acatggagcg ggtttgcgcc ttgtgccacg acaaacgcca atgcgaccgc gacctcgccg
     1621 ccggcgcctc cgccgagcgc tatgagggat attgcctgaa cgcgaccacg atcaacgctt
     1681 tggcccgaca gcccggcaaa taagcccgct gcgccaggca ctctcacaca gtgaatcaat
     1741 gaaaatatcg cgtgccgcct ggttccacgt ttttctgtcc gtcagtctcg ctctggccgc
     1801 gctgttcttg atccggctgc attatgcggg cggcgccgcg ctgtcctcag acagcgcgtc
     1861 cgacgggcat cgcctggcgg aggcctggtg caaggactgt cacgcgatcg aagcgacgac
     1921 ggcggggaca acgggagcgg caaacgccgc gccggctttc accgcgatcg ccaatcagcc
     1981 ttcgaccacc gagctttcac taaaagtgtt tcttcggtcg agccaccgaa gcatgcccaa
     2041 tctcctgatc acgcctgatc aggccgatgg tctcgcaaat tacattctca gtctgaagcg
     2101 caacttattt tgtttaatgc tgccccatcg ccgcgacagt tcttcttccc ctctcccctt
     2161 gtgggagagg gtggccgcga tgcgaagcat cgcggacggg tgaggggtta cggcctctcg
     2221 ataggcctga acccctcatc cggcgcggac ttcgcatagc cgatgcaagg catcggcgtc
     2281 ctcttaaagg acggcggccg aaggccgcct atggccacct tctcccacaa ggggagaagg
     2341 gaaggatcag tgcctgaggg aagaatcaat ggccgatcgc cgcgatcgca tccgcgatcg
     2401 cgatcgtgcg cctggccata tcggcgtgca gacgttccac catctggccg tcgagctgga
     2461 tcgcgccgcg cgaggcgttt tccgggcgtt cgaatgccac gatgatcttg cgggcatgcg
     2521 cgacttcctc cgccgggggc gtgaagatgg cgttgcaggc ctcgatctgg cccggatgga
     2581 tcagcgtctt gccgtcgaag ccgaggtcac gcgcctgcgc acattcttgt ccgaagccgt
     2641 cgacattgga aaaatcgctg tagggcccgt cgagaatttc aaggccgtgc gcgcgcgtcg
     2701 ccagaatgca gtgcgtgatc atcgggatca tcgccgcgcg gcccggccgc atccggatcc
     2761 gggtctcccg cgaaatatcg ttcggcccga acacgaagcc cgcgagccgc agctcggaat
     2821 cgcacgacga tgccgcaagc tcctcggcat gcagcaccgc gcgcgcggtc tcgatcatcg
     2881 cccagacccg gatcgaactg tccgcgttca tatcgccgag gcgatcggcg atggtggtga
     2941 gatccttcac gctggagatc tttggaacca ggatgccgtc gggcctggcc ttgccggcca
     3001 tggtgacgtc atcgatccac cagggcgaat ccagcgcgtt gacgcggatc agcacttcac
     3061 gcttgccgaa cccgcctgcg gcgattgcct gcgcgatctg ctcgcgcgcg gcagccttgg
     3121 cctccggcgc cacggaatct tcgaggtcga cgatgatgcc gtcggcgggg agattgcgcg
     3181 ccttttccag cgcccgcgca ttcgacccgg gcatgaacaa aaggctgcgg cgtggacgga
     3241 tcatgggttc atttccttgc agatctccag ccccacgcga tagcatttca gcgcgtctcc
     3301 cgaaagcctt gttcccgtca ccggcctatg gtttagccac cgcgatgtca tcctttccag
     3361 aacatctgct cgaaggctat cgcgccttca cctcgcagcg tctgccgacc gagcagtcgc
     3421 gctaccgcga actgtccgag ttcggccagt cgcccgaggt gatggtgatc ggctgctgcg
     3481 actcgcgggt gtcgccggag gtgatcttcg atgccggccc cggcgaattg ttcgtggtgc
     3541 gcaacgtcgc caatctggtg ccggtgtatg cgccggacgg cggcgcgcac ggcgtctcgg
     3601 cggcgctgga atacgcggtg cgggcgctgc acgtcaaaca catcgtggtg ctcggccacg
     3661 cccaatgcgg cggcatccgc gccttcgtcg acaatgtcgc gccgctgacg cccggcgact
     3721 tcatcggccg gtggatgtcg atgttcatca agccgggcga gaaagtcggg caacgcgacc
     3781 atgagacgat ggccgatttc accac
//
LOCUS       DASWGM010000025         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_971861,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000025 DASWGM010000000
VERSION     DASWGM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971861"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            692..3310
                     /locus_tag="VGA15_00855"
     CDS             692..3310
                     /locus_tag="VGA15_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9586279.1"
                     /translation="MARIKPEFDKRNVKIFGLSSDPVGNHAKWATDIGLAPSYPVIGD
                     TDFKIAKARGMLPAAVSGDASKRTAADNQTVRNAFVVGLDKKIKGCCGANDHSTHHGE
                     SDHLSLSFDDKVLDPVCGMAVDPATSQHRFDHHGRIHHFCSSGCRTKFAANPEHYLDK
                     AGSHTDVPAGTIYTCPMHPEIRQIGSGTCSICGMALEPEVATADAGPNPELIDMTRRF
                     WIGLALSAPVVVLEMGGHLVGGHGWIDQTISNWVQFVLATPVVLWAGWPFFIRGWHSV
                     LTRNLNMFTLIAMGVGVAYAYSVVATLAPGTFPTTFRGHDGAVAVYFEAAAVITVLVL
                     LGQVLELRAREATSGAIKALLDLAPKTARRLGADGTDEEVAIDVLQPGDHLRVRPGEK
                     VPVDGEIVDGRASLDESMVTGESMPVTKEAGGKVIAATLNRSGSFVMRAEKVGRDTML
                     SQIVQMVAQAQRSRAPIQRLADRVAGWFVPSVIIVALLAFGAWALYGPEPRFAFGLVA
                     AVSVLIIACPCALGLATPMSIMVGVGRGAQAGVLIKNAEALERMERINTLVVDKTGTL
                     TEGRPKVVSIVPASGYQADEMLRLAASVERASEHPLADAIVREASERKIATSDVKDFD
                     SPVGKGAIGTVDGNTIALGNTAFMASRKVDTSALEAEAEKLRGEGATVINMAIDGKLA
                     GILAIADPVKPSTPDALKALAAEKIRVIMLTGDNRTTAQAVARKLGIADVEAEVLPDK
                     KSAVVAKLQKEGKVVAMAGDGVNDAPALAAAEVGIAMGTGTDVAMESAGVTLLKGDLT
                     GIVRARRLSEATMSNIRQNLFFAFIYNAAGIPIAAGILYPTFGLLLSPIIAAAAMALS
                     SVSVVGNALRLRSANL"
     gene            complement(3404..>3667)
                     /locus_tag="VGA15_00860"
     CDS             complement(3404..>3667)
                     /locus_tag="VGA15_00860"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9586280.1"
                     /translation="GPYALMRHPQYTGIFLALFGEGVVHWPTLFSLTAFPIIVIAYSL
                     LARKEEQQMMKKFGGKYRAYQQRVPMFLPDRYDWRRVFSDYQI"
BASE COUNT          746 a         1116 c         1105 g          700 t
ORIGIN      
        1 atgatcgcgc acggcagcgc gaacaacaga ggcagccact ttccggcgtc ctgcgtaccg
       61 ccgagcgcac caccgaaaac aagaacggcg ccgacgatgg cgactgcgca gaccgcaagc
      121 ccgctccacc cgccgagcca actccgcgcg ctgcccgctg aagagccagg aggtccagtc
      181 gtcagattct tatttgtttc cattgtttca actcccttca agggacaagt aatccttcca
      241 atgattggaa ggtcaagggg ctatccggtt tttcgatcgc tttgttcaat ttcaattttg
      301 ggattggccc catcgtataa cgttaggcca tccccccacc cccgttcctg accaaaaata
      361 gacctgctga tttagaactg ggccaaacgc agtcggagca cgttaccagc cacctagatc
      421 gaattgaaac tctggccgag tgcacatgga caggcgatga ccagcccgac aaccgcatta
      481 actaatgttt agaaagggtc cttgaccttc caatgggggg aagccccacc ctcgtggtcg
      541 gactcaggca atggaggttc atgatggctt tacacattgt cgataccgcc ccggatttcg
      601 aagcgaaaac caccaaagga aaaatccgct tccatgcctg gatcagcgac gaatgggcag
      661 tgttgtttcc ctatcccaag gacttggtta catggcgcgg atcaagccgg aattcgacaa
      721 gcgcaacgtc aagatttttg ggctttcctc ggatccggtc gggaaccacg cgaaatgggc
      781 gacagatatc ggtttggcgc cgagctatcc ggtgatcgga gacactgact tcaagatcgc
      841 aaaagcccgg ggcatgctcc cggcagcggt ttcgggcgac gcgtcgaagc gaacggccgc
      901 tgacaaccag acggtgcgca atgcattcgt cgtcggactg gacaagaaga tcaaggggtg
      961 ctgcggagcc aatgatcact ccacccatca tggcgagagc gatcatcttt ccctcagctt
     1021 tgacgacaag gtcctagacc ccgtctgcgg aatggcggtc gaccccgcga caagccaaca
     1081 tcgcttcgac catcacggcc gaatccacca tttctgctcg tcgggatgcc ggaccaaatt
     1141 cgcggccaat ccagagcact atctcgacaa ggctggatcg cacaccgacg ttcccgcggg
     1201 cacgatctac acttgcccga tgcaccccga gatccgccag atcgggtccg gaacctgctc
     1261 gatctgcggc atggcgctcg agccggaagt agcgacggca gatgccggtc ccaatcccga
     1321 actcatcgac atgacgcgcc ggttctggat tggcctcgca ctatccgcac cggtcgtggt
     1381 gctggagatg ggcgggcacc tcgtcggtgg tcacggctgg atcgaccaga cgatctcgaa
     1441 ctgggtccag ttcgttctcg ccacccccgt cgtgctctgg gccggctggc cgttcttcat
     1501 ccgcggctgg cactccgtcc ttacccgcaa tctcaacatg ttcacgctga ttgcgatggg
     1561 cgtgggcgtg gcctatgcct acagcgtcgt cgcaacgttg gctcccggca ccttccccac
     1621 caccttccgc ggccacgatg gcgcggtcgc tgtgtatttc gaggctgctg cggtcatcac
     1681 cgtgctggtg ttgctgggtc aggttctgga gctgcgcgcg cgggaagcga cctccggcgc
     1741 gattaaggcg ttgctcgacc tggcgcccaa aacggcgcgc cgccttggcg ctgacggtac
     1801 cgacgaagag gtcgcgatcg atgtgctgca gccgggcgac cacttgcgcg tccgtccggg
     1861 agagaaagtg cctgttgatg gcgagatcgt cgacggtcgg gcgtcgctcg atgaatcgat
     1921 ggtgacgggc gagtccatgc cggtcactaa agaggccggc ggcaaggtaa tcgcggcaac
     1981 actgaaccgt tccggtagct tcgtgatgcg cgccgaaaaa gtcggccgcg acaccatgct
     2041 gtcgcagatc gtgcagatgg tagcgcaggc gcagcgctcg cgcgcgccga tccagcggct
     2101 tgccgatcgg gttgccggct ggttcgtacc gtcagtcatt atcgttgcac tgctcgcctt
     2161 cggcgcctgg gcgctgtacg gccccgagcc gcgcttcgcc ttcggcctgg tcgccgcggt
     2221 cagcgtcttg atcatcgcct gcccctgcgc gctcgggctc gccacgccga tgtcgatcat
     2281 ggtgggcgtt ggacggggcg cgcaggccgg cgtgttgatc aagaatgccg aggcgctgga
     2341 acgaatggag cggatcaata cgctcgtcgt cgacaagacg gggacgttga ccgaaggccg
     2401 cccaaaagtc gtatcgatcg tgccggcttc cggatatcag gccgacgaga tgctgcggct
     2461 tgccgcgagc gtggagcgcg ccagcgaaca cccgcttgcc gatgccatcg tcagggaagc
     2521 gagtgaacgc aagatcgcga cttcggacgt caaggatttc gactcgcccg tcggcaaggg
     2581 ggctattgga acggtcgacg gcaataccat tgctctcggc aacacggctt tcatggcatc
     2641 tcgaaaggtg gatacctcgg ctttggaagc cgaagcagaa aaattacgcg gcgaaggcgc
     2701 aaccgttatc aacatggcca tagatggaaa gctcgccgga atcctcgcga tcgccgatcc
     2761 cgtcaagcca tcaacaccgg acgcgctgaa ggcgctcgca gcggaaaaaa tccgtgtcat
     2821 catgctgacc ggcgacaacc gcacaacggc acaggcggtg gcgcggaaac tcggcatcgc
     2881 cgatgtcgag gccgaggttc tgcccgataa gaagagcgcg gtcgtcgcaa aattgcagaa
     2941 ggagggtaag gtcgtcgcga tggcgggtga cggcgtcaac gatgctccgg cgctcgcggc
     3001 agccgaagtc ggcatcgcga tgggcacggg aaccgacgtc gcgatggaaa gcgcgggcgt
     3061 tacgctgctc aagggcgatc tcaccggcat cgttcgcgcg cgccgcctgt ccgaggcgac
     3121 catgagcaac atccgccaaa acctgttctt tgcgttcatc tacaacgcgg cgggcatccc
     3181 gatagcggca ggtattcttt atccaacgtt tgggctgctg ctctcgccaa tcatcgctgc
     3241 ggccgcgatg gcgctgtcgt ctgtgagtgt ggtcggcaat gcactgcggt tgagatcggc
     3301 gaacttatga aaatctagtt tccgttttgc gccgccggag cactgacttg cgccagcgtg
     3361 ccaattcgcg tccagcacca aagctccgtc tattcgttgc gcctcaaatt tgatagtcgc
     3421 tgaagaccct ccgccaatca tatcgatcgg gcaagaacat cggcacccgc tgttggtacg
     3481 cccgatactt gccgccgaat tttttcatca tctgctgctc ttcctttcga gccaggagcg
     3541 agtatgcaat gacgattatg ggaaaggccg tcagtgaaaa gagcgtcggc caatgcacca
     3601 caccttcgcc aaagagcgcg aggaaaatgc cggtgtattg tggatggcgc atcagggcgt
     3661 aaggacc
//
LOCUS       DASWGM010000026         7063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_971912,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000026 DASWGM010000000
VERSION     DASWGM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971912"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGA15_00865"
     CDS             <1..891
                     /locus_tag="VGA15_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586281.1"
                     /translation="GGRKTVYLDAVRYRFMPEATTRIAALQNGEAQLISTIPNELSAR
                     ITERKDLAVRELFPLLGSYIIVNSQKGLTANVLIRQAIASAINVDEIIEGAGGINKRN
                     PWMSFPDTPYYLGEAAPTPWYDIKDIAKSRELLKQAGYNNEKIIIETNSNYQWMRSTM
                     LVVAEQLKAAGINVDVQVTDWTTNAAHMQQGTGDWNLSTTAFGPDHILGPQQWRPMLY
                     TFPHITGNDALDKAYSEFFAAPTLDQRRSAWLKIQQQVLGGAYLIKIADTGRLSGYAK
                     RLKGQSEYAGVLQLWDLWLE"
     gene            931..1875
                     /locus_tag="VGA15_00870"
     CDS             931..1875
                     /locus_tag="VGA15_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586282.1"
                     /translation="MLRIIAFKFLQAVPVILIISILAFLLLSLLPGDPAIIIAGEQAS
                     PEAVEHVRQQLGLDRPFLQQLAIWLLNLAQGNFGSSLILNQSVLSAVGERLPVTLSLA
                     ALSIAVTIPVGVFLGSLAAYYRQSWIDAGVMTLALLGVSIPAFWIAILGIILFSVHLG
                     WVPSSGFTPISQGVGPWLSSVILPALILSLFQIGFLARMTRSAMLDVMGQDFIRTARA
                     KGLSEWRTVGKHALRNALILIITATGILLSTAIGGSVVIEQVFALPGIGRMVVQAILA
                     RDYPLVQGTMLIFGFTFVLINLIVDVLYTLADPRVRHD"
     gene            1868..2758
                     /locus_tag="VGA15_00875"
     CDS             1868..2758
                     /locus_tag="VGA15_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014489672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586283.1"
                     /translation="MTDIGFEAIVSTAGLAPPRRGRSIRRLFRHRSFVIGFVIIVVLA
                     LAAIFAPLLTSLDPSAMKVRFRFRSPASDFIFGTDMFGRDIFTRVLYGARVSLFVGLA
                     VSVISGFCGAVLGVVAAQFRKLDAPIMRLMDALMSFPAILLALSIAAALGPRVSSVIA
                     ALTVAYVPAGVRIVRAAALVVREMDYVQAARLSGASALRIIVRHILPNCFSPLLVHLT
                     FIFAYAILAEAALSFLGVGVQPPLASWGNIIAEGRDYATEAWWVMLFPGIAISLAALG
                     MNLLGDALRDVLDPRLKGGD"
     gene            2760..3755
                     /locus_tag="VGA15_00880"
     CDS             2760..3755
                     /locus_tag="VGA15_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586284.1"
                     /translation="MAAAPLLVIRDLTVQFRVDAGWVTAVEGVSIDIAEGDCVGVVGE
                     SGSGKSVTALSILRLHDRATTRIPSGSIVFKGQDLLKSSKLSLRGIRGGEIAMIFQDP
                     MSSLNPVLTISEQISETIQQHQKMSGTAARRRAVELLDLVRIPDARRRADDYPHRLSG
                     GMRQRVMVAMAIACRPRLLIADEPTTALDVTIQAQILELLRELRKELGMSVMLISHDL
                     GVIAEFAQSVVVMYAGRVVERAPVAQLFRRPVHPYTIGLMAAVPKLDGGATRLSAIPG
                     RIPGPQEDIPGCRFAPRCGFVQNSCLRTPPPIELAGELHTVRCPPQVLALGDVNG"
     gene            3796..4719
                     /locus_tag="VGA15_00885"
     CDS             3796..4719
                     /locus_tag="VGA15_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586285.1"
                     /translation="MVSHAGGTSPLNALDDVNFDVMPGETLGLVGESGSGKSTAGRIL
                     VGLIPATAGTVSLFGRRITGRDGAAALRAVRSRLQFVFQDPYAALNPRMRVGSSVAEP
                     LEIAGKLSRRDRSDRVAELFELVGLPRISTDRYPHEFSGGQRQRIVIARSLALNPDFI
                     VCDEPVSALDVSMQAQIVNLLMDLQQRLGLTYLFIAHDLAVVRMISTRVAVMYAGSIV
                     ETAPKKDIYAQPQHPYTKALLDAVPRPDPAFVRAPVISGEVPSLLDPPMGCRFHPRCP
                     LVMDRCRTEVPRLAASGTDRQVACHLFAEKN"
     gene            4913..5686
                     /locus_tag="VGA15_00890"
     CDS             4913..5686
                     /locus_tag="VGA15_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9586286.1"
                     /translation="MPDILVTTRDSVGVVSLNRPQVRNAVTLRMWRELASIFSCLADD
                     DAVRAIVLTSSGADFCVGADVSEFGTIRENRDQSAEYEVSVDACSAAIANVSKPVIAA
                     ISGYCLGGGCHLALACDFRFADHTAKLGIPSARLSIVYGVNSVQRLLAIAGIANAKHM
                     LYSGERYDAARALSMGLVDEMSDDAVSAALAFAATLAGNAPLSIAGAKFMLNGLSMGE
                     GALDVAAAQRMIDDAADSEDFREGRRAFAEKRPPRFRGS"
     gene            5746..6621
                     /locus_tag="VGA15_00895"
     CDS             5746..6621
                     /locus_tag="VGA15_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9586287.1"
                     /translation="MKPAAFDYIAPNSLEAAVEAIAAANGDGKILAGGQSLMPLLNFR
                     MARPSVIVDLLHIPDMSFIERRGDTVAIGALTRHVDLEFSDLIAQKLPVMAAAMPHVA
                     HLAIRNRGTIGGSLSHADPAAELPMLAVFYGATIKVQGTSGRRDIPAEDFFVSALTNC
                     LDPDEIVFEIDFPALTSHAGWAFEEVARRFGDFALASIAVSFEFLDSRIVDARVAVMG
                     VADTPRRLREAEAALTGAQGGPKTAKKFAEVVRACVSPESDIHVSAEYRKNLIGALAE
                     RAFTTAWTRAVGAKP"
     gene            6624..>7063
                     /locus_tag="VGA15_00900"
     CDS             6624..>7063
                     /locus_tag="VGA15_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012707174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9586288.1"
                     /translation="MDIVVTVNGRKYQVSIEPRLLLSDFLRDTIGLTGTHVGCEHGVC
                     GACTILVNGDSVRSCLTLAVRADGMDIVTVEGLGTPDHLNALQTEFREKHGLQCGFCT
                     PGMLMTAMDLLKKYPLASDEEIREGLSGNLCRCTGYQNIVAAIRS"
BASE COUNT         1242 a         2129 c         2188 g         1504 t
ORIGIN      
        1 ggcggacgca agacggttta tctcgatgcg gtacgctatc gtttcatgcc ggaagcgacc
       61 acccggatcg ccgcgctgca gaatggcgaa gcgcagttga tttcgacgat tccaaatgaa
      121 ctcagcgccc gcatcacgga acgcaaggat cttgcggtcc gcgagctctt cccgctgctc
      181 ggaagttaca tcatcgtcaa ctcgcaaaag ggtttgacgg ccaatgttct gattcgccag
      241 gccatcgcgt cagccatcaa tgtcgacgaa atcatcgagg gcgcgggcgg catcaacaag
      301 cgcaatccgt ggatgtcgtt tcccgacacg ccgtattatc tgggagaggc cgcgccgacc
      361 ccctggtatg atatcaagga cattgcgaaa tccagggaac ttctcaagca ggccggatac
      421 aacaacgaga agatcattat cgagaccaac agcaattacc agtggatgcg atccaccatg
      481 ctggttgtcg ccgagcagct caaggctgcc ggaatcaacg tcgacgttca ggtcaccgat
      541 tggaccacca acgccgcgca tatgcagcaa ggcaccggtg actggaacct gtcgaccacg
      601 gcctttgggc cagatcatat tctgggtccc cagcaatggc ggccgatgct ctacacattt
      661 cctcacatca ccggaaacga cgcgctcgat aaggcctaca gcgagttttt tgcagccccc
      721 acgctggacc agcggcgttc ggcatggctg aagattcagc agcaggttct tggcggcgcc
      781 tatctcatca agatcgccga taccggaagg cttagcggtt acgcaaagag gctgaagggc
      841 caaagcgaat atgccggggt actccagctc tgggatctct ggttggagta agatccgaaa
      901 atcagcgtcg gccgtcgggg gagtttggct ctgctacgca tcatcgcctt caagttcctg
      961 caggccgtgc cggtgatact gatcatctcg atcctggcct ttctcctgct cagcctgctg
     1021 ccgggtgacc cggccatcat tatcgcgggc gagcaggctt cgccggaagc cgtcgaacac
     1081 gtacgtcaac agctcggcct tgatcgcccg ttcctgcaac aactggcaat ctggttgctt
     1141 aatctggcgc agggtaattt tggctcatcg ctgatcctga accagagcgt gctttcggcg
     1201 gtcggcgagc gcctgccggt tacgctttcg ctcgcagcgc tgtcgatcgc cgtcacgatt
     1261 ccggtcggcg tttttctggg ctcgctggcg gcctattatc ggcaatcatg gatcgacgcc
     1321 ggcgtcatga cgctcgcgct gcttggcgtg tcgatcccgg cattctggat tgccattctc
     1381 ggcatcatcc tgttcagcgt tcaccttgga tgggtgccgt cgagcggctt cacaccgatc
     1441 tcgcaagggg ttgggccgtg gctttcgtcc gtgatcctgc cggcgctgat cctgtcatta
     1501 tttcagatcg gctttctcgc gcgcatgacg cgctcggcga tgctcgatgt catgggccag
     1561 gatttcattc gaacggcgcg cgccaaaggg ctcagcgaat ggcgcacggt cggcaagcac
     1621 gcccttcgca atgccctgat tctcatcatt accgcgacgg gcatattatt gagcacggcg
     1681 atcggcggct cggttgtcat cgaacaggtg ttcgcgttgc cgggcatcgg ccggatggtt
     1741 gtgcaggcga tcctggcccg cgattacccg ctggtgcagg gaaccatgct gatcttcggc
     1801 ttcacctttg tactgatcaa cctgatcgtc gatgtactct acacgctcgc cgatccgcgg
     1861 gtgcgccatg actgatatcg gctttgaggc catcgtcagc acggcaggcc tggctccgcc
     1921 gcggcgtggc cgctcgatcc ggaggttgtt caggcacagg tcattcgtga tcggctttgt
     1981 gatcatcgtc gtgcttgcgc tcgcggccat ctttgcgccg ctgctgacct cgctcgatcc
     2041 ttcggcgatg aaagttcggt tccgctttcg cagccccgca tcggatttca tctttggtac
     2101 cgacatgttc gggcgcgaca tttttacccg cgtgctttat ggcgcccgcg tctcgttgtt
     2161 cgttgggctc gccgtctcgg ttatttccgg cttttgtggc gccgttctcg gggtcgtcgc
     2221 cgcgcaattc cgcaagctcg atgcgcccat catgaggttg atggacgcgt tgatgtcgtt
     2281 cccggccatt cttctggcgc tgagcattgc cgcggccctt ggcccacgcg tctccagcgt
     2341 gatcgccgcc ctgaccgtgg cctatgttcc ggccggcgtg cgcatcgttc gcgcagcggc
     2401 gctggtcgtg cgcgagatgg actatgtgca agcggcgcga ctgtcgggcg catcagccct
     2461 gcggatcatc gtgcgtcaca ttttgccgaa ctgtttcagt ccgctgcttg tgcacctgac
     2521 cttcatcttc gcctacgcca tccttgccga ggcggcgctg agctttctcg gcgtcggcgt
     2581 gcagccgccg ttggcaagct ggggcaacat catcgcggag ggacgcgact atgccaccga
     2641 ggcctggtgg gtgatgctgt tcccggggat agccatcagc ctcgctgcgc tgggcatgaa
     2701 tctactcggc gacgcgttgc gcgatgttct ggacccgcgc ctgaagggag gcgactagag
     2761 tggccgccgc cccgctgctc gtgatccgcg atctgacggt gcagttccgc gtcgatgccg
     2821 gttgggtgac ggcggtcgag ggcgtgtcga tcgacatcgc ggaaggcgat tgtgtcggcg
     2881 tggtcggaga atccggcagc ggcaagagcg tgaccgcgct ttccatcctg cggttgcatg
     2941 atcgtgcgac gacccgcatc cccagtggtt caatcgtatt caaaggacag gatctgctga
     3001 aatcgtcgaa gctttcgctg cgcggcattc gcggcggcga gatcgcgatg atctttcagg
     3061 atccgatgtc gtcgctcaat ccggtgctga cgatatccga acagatttcc gagacaattc
     3121 agcaacacca aaaaatgtcc gggacggccg cgcggcgccg cgcggtcgag ttgctcgatc
     3181 ttgtacgcat tcccgatgcc cgccgccgcg ccgacgatta cccgcatcgt ctgtcgggcg
     3241 ggatgcgcca gagggtcatg gttgcgatgg ccatcgcgtg ccggccgcgt cttttgattg
     3301 cggatgagcc gacgacggcg ctcgacgtca cgatccaggc ccagatcctc gagcttttgc
     3361 gcgagttgcg caaggagctc ggcatgtcgg tgatgctgat ctcgcacgac ctcggtgtca
     3421 tcgcggagtt cgcccagagc gtcgtcgtca tgtatgcggg acgtgtcgtt gaacgggcgc
     3481 cggttgcgca acttttccgc cggcccgtcc acccctacac catcgggctg atggccgcgg
     3541 tgccgaagct cgatggcggc gccacgcgcc tttcggctat tcccggccga atcccggggc
     3601 cgcaggaaga tatcccgggg tgtcgcttcg cgccgaggtg tgggtttgtg caaaacagct
     3661 gcctgcgaac gcccccgcca atcgaactgg cgggagagtt gcacaccgtc cgctgcccgc
     3721 ctcaggtgct tgctcttggt gatgtcaatg gctgacgcgc cgatcgtgga ggtgcgcggc
     3781 ctcaagaaga cattcatggt cagccacgcc ggtgggacgt cgccgctgaa cgcgctggat
     3841 gatgtcaatt tcgacgtcat gcccggtgaa acgctcggac tggtcggaga atccggttcg
     3901 ggtaaatcca ctgccggccg tattctggtc gggttaattc cggcgaccgc aggcaccgtt
     3961 tcgttgttcg ggcgccgcat caccggccgg gacggggcgg cggcacttcg cgcggtacgt
     4021 tcgcgtctgc agttcgtatt tcaggatcca tatgctgccc tgaatccccg catgcgcgtc
     4081 ggcagttcgg tcgcagagcc gctggagatt gccggaaaat tgtcgcgccg ggaccgaagc
     4141 gaccgggttg ccgaactgtt tgagctggtg ggactgccta ggatcagcac cgatcgctat
     4201 ccgcatgagt tttcaggcgg gcaacggcag cgcatcgtca tcgcccggtc gctcgcgctc
     4261 aatccggatt ttattgtctg cgacgagccg gtctcggccc tcgatgtctc gatgcaggcc
     4321 cagattgtca atctgttgat ggatttgcag cagcggctag gcctgaccta tctcttcatt
     4381 gcccatgatc tcgccgtggt tcggatgatc tcgacacgcg tcgcggtgat gtatgccggc
     4441 tccatcgtcg aaaccgcgcc gaagaaggac atctacgcgc agccgcagca tccctatacg
     4501 aaggcgctgc tcgatgcggt gccgcgaccg gacccggcgt ttgtccgcgc gccggtcata
     4561 tcaggcgaag tcccgagcct gctcgatccg cccatgggct gccggttcca cccgcgctgt
     4621 ccgctggtga tggatcgctg tcgcacggaa gtccccaggc ttgcagcgtc cggaacagac
     4681 cgtcaggtcg cctgccattt gtttgctgaa aagaactgag gcattttatc gcgagggcgg
     4741 atgcatgccc gcgagctgga gcttgacgaa ggacactttg taagtctact tatgcaagtc
     4801 acgccggctg cgagaccgga agtcttgttg ctgccaatca aatcttgttg ctgccaatag
     4861 cgtcgtgcag ggtttcgtcg ccaccaaccg cgacatcggc cgagggtcgg aaatgccaga
     4921 tatcctcgtg accacacggg atagcgttgg agttgtcagc ctcaaccggc cgcaggtgcg
     4981 caatgccgtg acgcttcgca tgtggcgcga acttgcaagc atattttcgt gcctcgccga
     5041 cgatgatgcg gttcgcgcga tcgttctgac cagttcaggt gctgacttct gcgtcggcgc
     5101 cgacgtgtcg gaattcggca cgataagaga aaaccgggat caaagcgccg aatatgaagt
     5161 ttcggtcgat gcctgctcgg ccgcgatcgc gaatgtgagc aagccggtga tcgcagcgat
     5221 ctccggctat tgccttggcg gcggctgtca cctggcgctc gcttgtgatt ttcgcttcgc
     5281 agaccacacg gccaaacttg gaatcccgtc tgccaggctt tcgattgtgt atggcgtgaa
     5341 cagcgtccag cgcctgctgg cgattgccgg tatagccaac gccaaacata tgctttattc
     5401 cggcgagcgc tacgacgcgg cgcgcgctct gtcgatgggc cttgtcgatg agatgagcga
     5461 tgacgcggtc tcagccgccc tcgcattcgc cgccacactt gccggcaacg cgccgctgtc
     5521 gatcgcggga gcgaaattca tgctgaacgg attatccatg ggcgagggtg ccctcgatgt
     5581 cgccgccgcc cagcgcatga tcgacgacgc cgcggatagc gaggatttca gggaaggccg
     5641 ccgcgcgttc gccgagaaga gaccgccccg gttccgcgga agctgaatgt cgcgcgtcgg
     5701 tagaagagtg agcgcgaggt cgagcgagct ttccggagaa gcacaatgaa gcctgccgcc
     5761 ttcgactata tcgctccgaa cagccttgaa gcagcggtag aggctatcgc ggctgcgaat
     5821 ggcgacggca agatcttggc cggcggacaa tctctcatgc ccttgctcaa ttttcggatg
     5881 gcgcgtccgt ctgttatcgt cgacctcctg catatccccg atatgtcgtt catcgaacgg
     5941 aggggcgata cggtcgcgat cggggctctg acgcggcacg ttgatctgga attctcggat
     6001 ctcatcgcgc aaaaacttcc ggtcatggcc gccgcgatgc cgcatgtcgc gcatcttgcg
     6061 atacgcaacc ggggtacgat cgggggcagc ctgtcgcacg ccgatccggc cgcggaattg
     6121 ccgatgctgg cggttttcta cggggccacg atcaaggtgc aaggcacaag cggccgacgg
     6181 gacattccgg ccgaagattt tttcgtatcc gctcttacaa attgcctcga tccggatgaa
     6241 atcgtcttcg agatcgattt cccggccctg acatcacatg ccggctgggc gttcgaggaa
     6301 gtcgcgcgcc gttttggcga ttttgcgctg gcgagcatcg cggtatcgtt cgaatttctc
     6361 gatagccgga ttgtcgacgc cagggtcgcg gtcatggggg tcgcggatac gccgcggcgt
     6421 cttcgagagg ccgaggcagc gctaacgggt gcgcaaggcg gccccaagac ggcgaaaaaa
     6481 tttgcggaag tcgtacgcgc ctgcgtttcg cccgaaagcg acatccatgt ttccgccgaa
     6541 taccgcaaga atctgatcgg cgcgctggcc gagcgagctt tcaccacagc atggacgcgc
     6601 gcggtgggag cgaagccgtg aagatggata tcgttgtcac cgtgaacgga cgaaaatatc
     6661 aggtgagcat cgagccgcgc ctgctgcttt ccgattttct gcgcgacacc atcgggctca
     6721 ccggcacgca tgtcggctgc gagcacggcg tatgcggcgc atgcaccatc ctggtgaacg
     6781 gcgacagcgt ccgctcctgc ctgacattgg ccgtcagggc tgacggcatg gacattgtga
     6841 cggttgaagg tctgggcacg ccggatcatc tgaacgccct ccagaccgaa tttcgggaaa
     6901 agcatggtct tcagtgcggc ttctgcacgc cgggaatgct gatgacggca atggatctct
     6961 tgaaaaaata tccactggcc agcgatgagg aaatccgcga gggactgtcc ggcaaccttt
     7021 gccgctgcac cggctatcag aatatcgtcg ctgcgatccg ctc
//
LOCUS       DASWGM010000027         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1526121,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000027 DASWGM010000000
VERSION     DASWGM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526121"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VGA15_00905"
     CDS             complement(<1..1329)
                     /locus_tag="VGA15_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9586289.1"
                     /translation="MTAQPLLDVEDLTVEFATRRGIVKAVQHVNISVAKGETLGIVGE
                     SGSGKSVTSYAVMRILDRAGKIAEGSVMFSGIDVKSATENQMRDLRGREMSMIFQSPR
                     LALNPIRKIGRQIEDVLRQHVQSAASDRGEKAIEALEQVRIARPRERYHAYPFELSGG
                     MCQRVVIALALACNPQLLIADEPTTGLDVTTQKAVMDLIVELTKRRGMSTILITHDLG
                     LAAAYCDRVVVMEKGRVVETALSADIFARPEHAYTKKLMRATPRLGVSLRDLLPEEEA
                     VSLTSPCLRGEVGLQSNPGEGVVDLTAVPDGAPHPDPLPAGGEREKKPGGDGAASGDA
                     STKPLLLVEKLVKEYPRQGATATLGKLFGRKPPVETEQFRAVDGISFTVGHGESVGLV
                     GESGCGKSTTSMMVMRLLDQTSGRIVFDGDEIGAILPNAFARLPLRKSIQM"
     gene            complement(1469..2371)
                     /locus_tag="VGA15_00910"
     CDS             complement(1469..2371)
                     /locus_tag="VGA15_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586290.1"
                     /translation="MSEASLTAAPTAEVQPKPPANSIFRHARYVVAENPVTGLSFGLF
                     VIIALCALIGPYIAPYDPLASDTSATLQSPSLHHWFGTDLLGRDIFSRVLVATRLDFV
                     IAVASVALVLVMGGLAGVAAGFFGGWTDRIVGRISDTIMAFPLFVLAMGIVAALGNTV
                     TNIVIATAIINFPLYVRVARAEANVRRDAGFVQAARLSGNSDFRIVMVHILPNIMPIM
                     MVQISLTMGYAILNAAGLSFIGLGVKPPTPEWGIMVAEGATNIISGEWWIAFFPGGAL
                     MIAVFCFNLLGDGLRDLVDPQRRT"
     gene            complement(2368..>2763)
                     /locus_tag="VGA15_00915"
     CDS             complement(2368..>2763)
                     /locus_tag="VGA15_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586291.1"
                     /translation="AATLAIFSLAPITRMTRASMLAVLSSEFIRTARASGLDDRTVIL
                     TYGFRNAMLPVVTTLGMVFSFLLGANVLVEKVFAWPGIGSYAVEALLQSDFAPVQGFV
                     LTMAVLYVALNLLIDMLYGVIDPRVRLEA"
BASE COUNT          546 a          897 c          854 g          466 t
ORIGIN      
        1 catctggatg ctcttgcgca gcggcagccg tgcaaacgca ttgggcaaaa tggcgccgat
       61 ctcgtcgccg tcgaacacga tgcggcccga ggtctggtcc aacagccgca tcaccatcat
      121 cgacgtggtg gatttgccgc aaccggattc gccgaccagc ccgacgctct cgccatgccc
      181 cacggtaaag ctgatgccgt cgaccgcgcg gaattgttcg gtttcgaccg gcggcttgcg
      241 cccaaacaat ttgccgagcg tggcggtcgc gccctggcgc ggatattcct tgacgagttt
      301 ttcgacgaga agaaggggtt ttgtactagc gtcgccactt gctgcaccgt cacccccggg
      361 cttcttctcc ctctccccgc ctgcggggag agggtcgggg tgaggggcgc cgtcaggcac
      421 cgcagttaag tccacaaccc cctcacccgg attgctttgc aatccgacct ctccccgcaa
      481 gcagggcgag gttagggaaa ccgcttcctc ctccggcaat aaatctcgca acgaaacacc
      541 gagccgcggc gtcgcacgca tcagcttctt ggtgtaggcg tgttccggcc tggcaaagat
      601 atccgccgac agcgcggttt cgaccacccg ccccttctcc atcaccacca cgcggtcgca
      661 ataggccgcg gctagcccaa gatcatgcgt gatcaggatg gtcgacatgc cccgccgctt
      721 ggtgagttcg acgatcaggt ccatcaccgc cttctgcgtg gtgacgtcga gaccggtggt
      781 cggctcgtcg gcgatcagaa gctgcggatt gcaggccagc gccagcgcga tcacgacgcg
      841 ctggcacatg ccgcccgaca gctcgaacgg gtaggcatga tagcgctcgc ggggacgtgc
      901 gatcctgacc tgctcgagcg cctcgatcgc cttctcgccg cgatcgctcg ccgccgactg
      961 cacatgctgg cgcagcacgt cctcgatctg gcggccgatc ttgcggatcg gattgagcgc
     1021 cagccggggg ctctgaaaaa tcatcgacat ttcgcggccg cgcaggtcgc gcatctggtt
     1081 ctcggtcgct gacttgacgt cgatgccgga gaacatcacc gagccttcgg cgatcttgcc
     1141 ggcgcggtcg agaatccgca tcaccgcgta ggaagtgacc gatttgccgg agccggattc
     1201 gccgacgatg ccgagcgtct cgcccttggc cacggagatg ttgacatgct gcaccgcctt
     1261 gacgatgccg cgccgggtgg cgaattccac cgtgaggtcc tcgacgtcaa gcagcggctg
     1321 cgcggtcata gcgtgtttcc gatttctagc tcgtcatgcg cgggcttgac ccgcgcatcc
     1381 atcaagaatc cgaagatgct cttcgagcga tggattgccg ggtcaagccc ggcaatgacg
     1441 acaatgtctg gggctgctcc gcccatgctc acgtcctccg ctgcggatcg accaggtcgc
     1501 gcaacccgtc gcccagcagg ttgaagcaga ataccgcgat catcagcgcg ccaccgggga
     1561 agaatgcgat ccaccactcg ccggaaataa tgttggtcgc accttccgcg accatgatgc
     1621 cccattcggg ggtcggcggc ttgaccccga ggccgatgaa cgacagaccg gccgcgttca
     1681 gaatggcgta gcccatggtc agcgatatct gcaccatcat gatcggcatg atgttcggca
     1741 ggatatgcac catcacaatg cgaaaatcgc tgttgccgga cagccgcgcc gcctgcacaa
     1801 agccggcgtc gcgccgcaca ttggcttcgg cgcgcgcgac ccgcacatag agcggaaaat
     1861 tgatgatcgc ggtcgcgatg acgatgttgg tcaccgtatt gcccagggcg gcgacgatac
     1921 ccattgccag cacgaacaac ggaaacgcca tgatggtgtc ggagatgcgt ccgacgatgc
     1981 ggtcggtcca cccgccaaag aagccggcgg cgaccccggc gaggccgccc atcaccagca
     2041 ccagcgccac cgaagcgact gcgatcacaa aatcgaggcg ggtcgctacc agcacgcggc
     2101 tgaagatatc gcggcccagc aaatcggtgc cgaaccaatg gtgcaacgac ggcgactgca
     2161 gggtcgccga cgtgtcgctg gcaagcggat cgtatggcgc aatgtaagga ccgatcaagg
     2221 cgcaaagcgc gatgatgacg aacagcccga acgacaagcc ggtcaccgga ttttccgcca
     2281 ccacataacg ggcatggcgg aaaatggagt tggccggagg cttgggttga acctcggcgg
     2341 tcggagctgc ggtcagactg gcttcgctca tgcctcgagc ctcacgcgcg ggtcgatcac
     2401 gccgtagagc atgtcgatca aaaggttgag tgcgacgtag agcaccgcca tggtcaacac
     2461 gaagccctgc accggcgcga agtccgactg cagcagcgcc tcgaccgcgt aggagccgat
     2521 gccgggccat gcgaacactt tttccaccag cacattggcg ccgagcagga acgagaaaac
     2581 catgcccaac gtggtcacga ccggcagcat cgcgttgcgg aagccgtagg tcaggatgac
     2641 ggtacggtca tcgagaccgc tggcgcgcgc ggttcgtatg aactcggacg acagcaccgc
     2701 cagcatcgag gcgcgggtca tgcgcgtgat cggcgccagc gagaaaatcg ccaacgtcgc
     2761 cgc
//
LOCUS       DASWGM010000028        33078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1248849,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000028 DASWGM010000000
VERSION     DASWGM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 33078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33078
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248849"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGA15_00920"
     CDS             complement(<1..602)
                     /locus_tag="VGA15_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX9586292.1"
                     /translation="MSTALRIVHGNFGRVALLDMDCSLVRHAHPHCHVLFKVAGADTQ
                     FVVGNRIHALTDSAAVLVDGWQPHSYVHDPARPRTLILALYVEPGWLKAFRPGWAASG
                     APGFFGTPHGEVSPGIRKLTMDLASEMMADPECQAEYEALLSELMIAVIERFTPWKSF
                     PRSIRELNVSACDRRIRRAAEYIRSNAGGEASIDTLAKEAG"
     gene            960..2150
                     /locus_tag="VGA15_00925"
     CDS             960..2150
                     /locus_tag="VGA15_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HEX9586293.1"
                     /translation="MTSTETISFELDQPALQARRNAKWNQYDADILPAFVADMDFSVA
                     APIQAAIERIVRDRDYGYALRNGEKPDRLVASAFAQRMKSRFGWELSPDLVLPLADLV
                     QGTYAPILAFSDPGDGVVLQTPNYPPFRDAISSTERELLALPMRDDGTRFVFDMNELE
                     KLVDKRTRIFILCNPQNPTGRVFSRDELLALGRFAIEHDLVVISDEIHSDLVFPGLQH
                     IPFASLGPEIAARTVTLNSATKSFNIPGLRCALMAFGSEDLRHRFHKRIPQRLTGSAN
                     IVGVDATVAAWSECQPWLDAVMEHLLEARNRVKDVLAAEVPEIRFHAPEATYLAWLDC
                     SKLRLSTPAFQFFLNNARIGFSAGETFDPDCAQFVRFNFATSMPILDDILDRFVAATR
                     QNDR"
     gene            complement(2187..4022)
                     /locus_tag="VGA15_00930"
     CDS             complement(2187..4022)
                     /locus_tag="VGA15_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX9586294.1"
                     /translation="MSDSLALGNTPLSYGPSAGESAFTTRPEIVGAFGVVASTHWLGT
                     AAGMRILEKGGNAFDAAVAIGFALQVVEPHLNGCGGEVPIIFHDARAGRARVLCGQGV
                     APAAASIERFAELDLDIIPGSGFLPACVPGSFGAWMTLLRDYGTMHVADVLETAITYA
                     RDGYVMLPRIAAAIEMVAPLFRTEWPTSAELYLRNGVPSAGTLFTNPALASTYERIVL
                     ISRDTPGNREQKIEAARKAFYEGFVAEAIDKFVRTPAMDTSGRRHAGLLTGQDLSTWQ
                     ATYEEPLSLSYGAFEVLKPGPWTQGPVLLQQLALLKGFDLSDLDPVGGEFVHLVTECS
                     KLAFADREKYYGDPDFVDVPMARLLSSEYADQRRRLVGNTASLEFRPGSIAGYGREVP
                     YVVARTGEPAVALGAGEPTVGPRGSVRGDTVHLDVIDRHGNIVSATPSGGWLQSSPAI
                     PGLGFALGTRAQMFWLKPGLASSLEPRKRPRTTLSVSLLTRDGKPYGSFGTPGGDQQD
                     QWSSVFFLRHAHHRMNLQSAIDAPMFHNDHMPSSFFPRQAKPGSLTLESRFSDAVLKD
                     LVGRGHRVEIVDAWSLGRISAVSKDGRILRAAANPRYMQGYAFGR"
     gene            complement(4068..4637)
                     /locus_tag="VGA15_00935"
     CDS             complement(4068..4637)
                     /locus_tag="VGA15_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEX9586295.1"
                     /translation="MLDLSARRSHSLAELAGSAAHVPTAMLAVLPDAAKLSFRGRPSS
                     NPAAGEAFGVSFPQTACRFAANGNRAAFWLGPDEWLLQATGEQASVLFAVMEEALLGY
                     SCSLVDVSHRSDAFALSGRMSDYVLNHGCPLDLSHQAFPVGMCTRTILGKATILLSRP
                     EPDTFHIDVWRSFAPYVWLLLDEARRELA"
     gene            complement(4630..7635)
                     /locus_tag="VGA15_00940"
     CDS             complement(4630..7635)
                     /locus_tag="VGA15_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HEX9586296.1"
                     /translation="MTAAYRLEAGGRIDRSRPVRFTFDGKRYDGFAGDTLASALIANG
                     MHLVGRSFKYHRPRGIMTAGSEEPNALVGVGADAARYTPNLRATQIEIHDGLVAESQN
                     RWPSLKFDVGAASESVASFLPAGFYYKTFMWPKAAWNRLYEPLIRRAAGLGRAPVRPD
                     ADRYTQVFVHCDVLVVGAGPAGLAAALAAARSGAKVIVCDEQAEMGGSLLTETTAVID
                     GKPAADWVMTTLGELALSRNVTLMPRTTAFGWYPHNFLGLCQRVTDHCASPDPKQPRE
                     RLWQVRAKEVVLATGAIERPMVFPDNDRPGVMMAEAARAYANRYGVRPGDKALVFTAC
                     DSAYRAAIDLTAAGVAIVAIADLRTAPGSLWVKRARAAGIDVRPSTVVTATRGRLRVR
                     SASLARAATGGGLGKAETVACDLVLMSGGFTPSVHLFSQSRGKLVYDDALQAYVPGTS
                     VERERSVGGCRGVYDLEKVLHDGFAGGMNAAVAAGANKTIEHRFAVKSDDVGIASFIG
                     ATPHGRNPLKTRAWIDFQNDVTAKDIALATREGFRSIEHVKRYTTTGMATDQGKTSNM
                     NALGVVSDALNLPVPSIGLTTFRPPYTPTTFGVFAGQSRGGLFDPERKTSIHGWAAEN
                     GAVFEDVSLWKRARYFPRAGEDMHAAVTRECKATRQSVGIFDASTLGKIEVVGKDAAE
                     FMNRMYTNAWTTLEPGRLRYGVMLREDGFIMDDGVVGRIAPDRFHVTTTTGGAARVLA
                     MMEDYLQTEWPDLEVWLTSTTEQWAVIAVQGPNARKFLEPLVQDIDLSRQAMPHMSLR
                     EGKICGVPTRLFRVSFTGELGFEVNIPQGYGRAVWEAIIGEGRSHGITPYGTEAMHVL
                     RAEKGYIIVGQETDGTVTPDDAGLNWAVGKSKKDFVGKRSLVRPAMTRSDRKQLVGLL
                     TVDPKTLLEEGAQIVADPAEPVPMTMIGHVTSSCWSAALDRSIALALVRGGRARIGAR
                     LSLPMPSGPIAVEVVAPVFYDPKGERLDA"
     gene            complement(7632..7928)
                     /locus_tag="VGA15_00945"
     CDS             complement(7632..7928)
                     /locus_tag="VGA15_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX9586297.1"
                     /translation="MLLICCPYCGPRSELEFAYGGEAHIARPADPMSLDDEAWANFLY
                     MRRNPKGMHAERWRHLHGCGRFFNARRDTVTDTIAATYPAGEQPPVAASKETKR"
     gene            complement(7947..9200)
                     /locus_tag="VGA15_00950"
     CDS             complement(7947..9200)
                     /locus_tag="VGA15_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX9586298.1"
                     /translation="MQKFSALSLFRGGLDGHAKWQRQWRSPEPRPHYDVVIIGGGGHG
                     LATAYYLAERYGISNVAVVERGWLGGGNTGRNTTIIRSNYLFDESARIYEHALKLWEG
                     LSQALNYNVMYSPRGVLMLAHTVHDVQSSRRHVHANRLNGIDNEWLSPQQCKDFCPPL
                     NVGGAMRYPILGGALQRRGGTARHDAVAWGYARAADARGVDILENCEVTGIRRGASGA
                     VEGVETSRGFIAARKIGIVAAGNTSMVANMAGIRLPLESFPLQALVSEPVKPALPCVV
                     MSNTIHAYISQSDKGEMVIGAGTDAYTSYSQRGALHIANHTIDAICELFPMFRRLRML
                     RNWGGVVDVTPDRSPIIAKTPVAGLYLNCGWGTGGFKATPGSGDVFAWTIARDEPHPI
                     NAPFSIERFRDGVLIDEAAAAAVAH"
     gene            complement(9283..10521)
                     /locus_tag="VGA15_00955"
     CDS             complement(9283..10521)
                     /locus_tag="VGA15_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9586299.1"
                     /translation="MSMPKAGAPESPALHLQGRPVLRVLGTEITLLESIRLHAEADLG
                     ITLVFENLDFISAQRKAAIQPETYDVYDQCFHNLDIVWFWRAIQSIDLKRIVRWNEVS
                     DLTKTGRIHSGAAIGRGDAPVTKLFAQPNRSLGSAPSGRISMLPTVHNLDSFAYNAGL
                     VNGHLEPSASWGALLDDRWAGRVALVDEPAIGVFDAALAAQARGEVHFDDIGNMSTAE
                     IDCLIEFLAERKRAGYFCGFWTTASEAADLMLSGQSIMQSMWSPGVSLLRRRDFPVRE
                     AVPQEGYRAWHGGLCLARHLSGRTLDIAYEYLNWWLDGWAGAVVARQGYYMSIPERVR
                     QHLSLAEWNYWYEGEPAAEDLPGPDGERTIGRGEVRSGGAYRQRASRIAVWNTTMDEH
                     NYLVRRWAQLVGSSAKAPLH"
     gene            complement(10570..11562)
                     /locus_tag="VGA15_00960"
     CDS             complement(10570..11562)
                     /locus_tag="VGA15_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9586300.1"
                     /translation="MVEDIPAFTRVTLLTGFLGSGKTTLLRRLLADPALKDTAVIINE
                     LGEVGIDHLLVERLDDQMVLLSSGCVCCTVRGELATTMRDLHSRRERGLVPPFRRLVI
                     ESTGLADPFPVLSTLKADPVLRHHFRAAGVVTTVDAVNGFGQLDRYIESVRQVAVADA
                     IVLTKSDIAEEAVTARLAARLDAINPSAPRLSAADAPLDADALLQAGGSGWFKAIAEP
                     SMAMAAHAGVRAFSIIVDAPLDWTAFGVWLTMLLNRHGARILRVKGILALEGEELPVA
                     VHGVQHLVHAPTHLSRWPSEDHRSRLVFIVEGIEPDLVRRSFSIFNRLNMSVAA"
     gene            complement(11555..11884)
                     /locus_tag="VGA15_00965"
     CDS             complement(11555..11884)
                     /locus_tag="VGA15_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011648931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586301.1"
                     /translation="MCSLCGSLGGNDHWADAAAREGVYTRNADPVRRRRERADRVRLA
                     NRVLALYGMRLSDWQGTSFLLSTLTGKSEIVEDLGHLWPAAEKLSGRICDPLAPDLLE
                     ALDADHG"
     gene            complement(11915..12796)
                     /locus_tag="VGA15_00970"
     CDS             complement(11915..12796)
                     /locus_tag="VGA15_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX9586302.1"
                     /translation="MAKEILVGFGVDVDAVAGWLGSYGGEDSPDDISRGLFAGEVGSV
                     RVLQLFERLGIKTTWFIPGHSIETFPKQMQAVADAGHEIGIHGYSHENPIAMTREQET
                     IILDKCIDLVTRLSGKRPTGYVAPWWEFSTVTNELLLERGIKYDHSLMHNDFHPYYVR
                     VGDSWTKIDYSKNPSAWMTPLKRGVETDLIEIPANWYLDDLPPMMFIKKSPNSHGFVN
                     PRHLEEMWRDQFDWVYRENDYAVFPMTIHPDVSGRPQVLMMLERLYKHIASHPGVRFV
                     TMNEMADDFARRSPRKQ"
     gene            complement(12840..13946)
                     /locus_tag="VGA15_00975"
     CDS             complement(12840..13946)
                     /locus_tag="VGA15_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586303.1"
                     /translation="MDFDLQLRHVRKVYENGTPAVVDFNLDVKKGEFIAFLGPSGCGK
                     TTTLRMIAGFEEITSGDILIRGRRINNVPPERRPTSMIFQNYALFPHMSVRGNVGYGL
                     DVKGMPKAERNAKVDGILEKFGLSVVADSLTHKLSGGQRQRVALARGLVVEPDILLLD
                     EPLGALDANLRKTIQAELKLLQRNLGITFVFVTHAQSEALTLSDRIVVMNQGKIEQVS
                     SPLDLYTRPKTPFVAQFVGRNTLIDGSLIKVEADRATLSTAFGEFVGTPDDRAAVGQA
                     VKIVVPSESLEIYPSAGGMREALAGQYQGNVLPGVMERADVVGHLVQMAITLAGGSTV
                     ILEAHVDKYRRNDLVPSSSVWIAWHPHNATIVAA"
     gene            complement(14029..15264)
                     /locus_tag="VGA15_00980"
     CDS             complement(14029..15264)
                     /locus_tag="VGA15_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011648934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9586304.1"
                     /translation="MVDKLELTRRKLFQGSAGAIALALGGGTPFVSSRTAYAAASDVA
                     KQQLRTIGLSVTVQDRILADYKAVSGVGSTAGTAATFPDAQTKILSGSKDFDCWETIG
                     ERLPAVTQTNKIEPIPAASLKNWANIRDTFTKSEAKFDKKAQITGQIWADDAQTKLWM
                     VPAVYNYDSIGYNPSVLSDEEANSWTAIFDKKWKGKSGLNTDPLIALGQAIMAMNTLG
                     LSDVKNPGNASKKQIEDAMKFLVSKKKEGQFRALWGDFGELVNLMASGEMVVCDAWQP
                     AVMAVKAQGKPAKYAVPKEGYRAWSIGPSMIVGSPNQEAVYAYADYWLSGTPGIIVSE
                     QGYYSPSTNIKNAMPADKYAFWYEGKPWKGKSENGIKEGDLRDGGSLETRAANVAYWH
                     QWPDEYDLVIQKWDEFLSA"
     gene            complement(15327..16106)
                     /locus_tag="VGA15_00985"
     CDS             complement(15327..16106)
                     /locus_tag="VGA15_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011648935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9586305.1"
                     /translation="MTDGKIASTKPTVLITGSGIGIGRATAFAFGKAGYHVVVTDVLE
                     QEGRAVAADIASAGGSAEFHLLNVRSTADVDRLVQDLCARFGALDCVVANAGIAHKVL
                     LEDLSDEKWDETFDIDLKGMLRVIRSALPGMKARKAGAIICISSIMGVAYGWDEHVHY
                     SSAKAGVVGLIRGLAVELARNGIRVNGIAPGYIRTAQLLSEKHSLGPAGAEKAGEFIP
                     MGRIGEPDEIADVILFLASSAARYMTGQTLVVDGGLLVGRY"
     gene            complement(16103..16840)
                     /locus_tag="VGA15_00990"
     CDS             complement(16103..16840)
                     /locus_tag="VGA15_00990"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004112175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9586306.1"
                     /translation="MAQEVAIVTGAGSGIGRAVAHRLARDGMAVVVNDVSGERAQLVA
                     QEICASNCLAKAIAGDVSREDDVAAITAFARAAFGDCSVLVNNAGIVHQARFVDLTAA
                     DFDRMFAVHVRGAFLCTRAVLPAMLAKERGIVINVASQLGQIGGVELVHYSAAKAAII
                     GMTKSLAREVSAQGVRVNAVAPGPINTPLVMALSKDWREAKAKELPLGRFGEPDEVAA
                     TVSFLASPEASLYVGQTLGPNSGDVML"
     gene            complement(16840..17685)
                     /locus_tag="VGA15_00995"
     CDS             complement(16840..17685)
                     /locus_tag="VGA15_00995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586307.1"
                     /translation="MQATFIKPVLAVYVALFLVFLYGPFLVLAILSFQTGPNGGPQFP
                     IIEWSTYWYRDIFGLAPPSRIAPLPIRDSLIRSLLLAMMTMVVSTVLGVMSAQAFRTH
                     FKGSGVIFYLIVLGMMVPGVLVGLGLALVANILGIDRTWWGTAFVLHVVYTFPFAFLV
                     MLAIFNRFDASVEEASWSLGVSPIRTFQKVTFPLIFPGVLSAMLFAFTLSYDEFSRTL
                     FSSGPDLTLPLAIYGTFSIEIHPNIFAFGVLTTLFSFALLAVYAILMTLSVRRARRIA
                     IQEES"
     gene            complement(17690..18595)
                     /locus_tag="VGA15_01000"
     CDS             complement(17690..18595)
                     /locus_tag="VGA15_01000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586308.1"
                     /translation="MTVLLHAAIDKPSLPVAGRRGLSRVAYWLIAPGVLWMTLFLVVP
                     ILMIVYVSFWTQTTFKIEPILTTRSWAAFFASDTYIGALWTTVRIWLIVLFTTLLVGY
                     PTALFVGLFVKNKTLSTALLVLCVIPFWTSFLIRVLAWRPMLGKEGAINIILQMLHIT
                     SAPIDALLFTELSVIIGMTQIYCVFMVGPIAFMLGRIDPNVIEAARDLGASFGLIFRT
                     IILPLSMPGVIVGSIFVSVMVLGEFATASALSGRKVNMLGNIIVTQVGSLKWAFASVV
                     GVILTILMGIVIAGLLRIVDLKREL"
     gene            complement(18632..19708)
                     /locus_tag="VGA15_01005"
     CDS             complement(18632..19708)
                     /locus_tag="VGA15_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586309.1"
                     /translation="MVTGVLHIAGLTKRYATTVALDNINLDIPGNCYVSLLGASGSGK
                     TSLLRIIAGFEQPDAGAIVFAGRQMVGTPTYSRGIGFVFQNFALFPHLSVYANIAFGL
                     ENRAEGAPLGASEVRDRVRAMIDLVGLAGLESRGVHQISGGQRQRVALARTLVTEPRL
                     VLLDEPLGALDANLRMRMRGELRRIRSRLGVTFLHVTGSEAEALAMGDRVVVLDRGRV
                     DQFDAPDVVFNQPASPRVAKSLNCYNLFRGEIDAGLFITEDHRFRLGGSRTSSADTSY
                     AIRQDMIAIREGEATNEPDEESISARYLASEYTGSAFMYFFQLHSGSIVEVENHLSHR
                     AMQNLDVNADYTLTWKTESALVFG"
     gene            20148..21170
                     /locus_tag="VGA15_01010"
     CDS             20148..21170
                     /locus_tag="VGA15_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004112167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9586310.1"
                     /translation="MKRPANPRPASRRYEMVEEVLRQNILGGLIPRGLVLLEGPIAAL
                     MQTSRAPVQTALQRLEEQGMVRRFRGRGFMVAKPGEVLEPIRLDLQGIGLRIPKAIDD
                     ALQSRASWERIYEIVEHDVAACLVFGRYRLIEAELATHFRVSRTVVRDVLGRLEQRGL
                     VIKNQSSHWIAEPLTAQSIREHFALRRILEPQALTSAAPLLDRTRLAAVFDRFRQAEA
                     AFDADHLNDPLARDLEAFETLLIDTCVFATPNRRLREQIRHNLLPVLAAERALRQLGL
                     PSDREAVTEHRLIVELLLQDAIPAAAAMLDAHLAASERRSIAQLKIVAVLSEPQSVAR
                     YLTRLD"
     gene            complement(21194..22165)
                     /locus_tag="VGA15_01015"
     CDS             complement(21194..22165)
                     /locus_tag="VGA15_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX9586311.1"
                     /translation="MTVKVLNPGLSTTVQDLGRPGYFHLGIPIGGAMDRLALRAANLL
                     VGNDEGAAGLEAVFIGPELEFTLDAVVAVTGAEMPIKIDGAEKPSWTALRIKAGQILS
                     FGYLQKGARIYIAISGGLDVPLALGSRSTYPIGALGGYKGRAIAKSDELPVGTVAFAG
                     EGRTIPERLRRMPAMPAELRVLPGLYWHRVVEESQQGFFDDPWKVAPEADRMGYRFRG
                     GRPLKFVEREQPFGAGSDPSNIVDACYPYGSIQVPGGTEPIILHRDAVSGGGYFMLGT
                     VISADMDLIGQLQPNTPTRFVKVDMQQALAARRDRQGVMQSIRDILV"
     gene            complement(22155..23030)
                     /locus_tag="VGA15_01020"
     CDS             complement(22155..23030)
                     /locus_tag="VGA15_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HEX9586312.1"
                     /translation="MSNRYSFGGDEHLFVECDESMSLEAFFKSLSMTSAVRESRIAGV
                     TEICPANASFQIKFDPDVIKPDDLLREVTAIEGAAEKTEPVIKTRIIEIPVFYQDPWT
                     HETLMRFRERHQDPKATDLEYAAQVNGLSGVDAFIAAHSGSPWFVSMVGFVAGLPFLY
                     QMVDRPRQLQVPKYLRPRTDTPKLTIGHGGCFSCIYSVRGAGGYQMFGITPMPIFDAE
                     QKISYLRDFMVFFRPGDIVKFKPIGRDDYDRAVEDVAAGTFAPTIRSVTFDLREFQKD
                     IDGCNAKLEGALDDR"
     gene            complement(23030..24412)
                     /locus_tag="VGA15_01025"
     CDS             complement(23030..24412)
                     /locus_tag="VGA15_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9586313.1"
                     /translation="MNIRSVLIANRGEIAVRIIKAAKALGLRTVQVYSQADAKSLAVR
                     LADEAIEIGPPAARKSYLNVDAILAAAAAAGVDAVHPGYGFLSENGDFADAVVAAGMI
                     FVGPSGAAIRLLGDKVAARQVAAMAGVPTVPGSDGRVLDLIEARAIVSKIGFPVMIKA
                     AAGGGGRGIRIAQDFDEFDRQFPQAAAEATAAFGDGGLYLEKVIEKARHVEVQILGDG
                     TNFVHCFERECSLQRRRQKVWEEAPAVGLPAGVREKLCASAVALARSVGYQGAGTVEY
                     LYDEATKAFYFIEVNTRIQVEHPVTEMVTGLDLVQEMLRIAGGLPLSVGQDEIAIRGH
                     AIECRINAEDPDRNFMPWPGVVEKLETPSGDGIRFDTLLFDGYEVPPFYDSLLGKLIV
                     HGANRTDCLARLRAALRSLKIVGIPTTLPLHTALALDPDVVAGRFHTRFLEQWLEARF
                     AAPASVKEVV"
     gene            complement(24409..24651)
                     /locus_tag="VGA15_01030"
     CDS             complement(24409..24651)
                     /locus_tag="VGA15_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEX9586314.1"
                     /translation="MSKLEIKSPLPGMFYRRPSPEAPPFRNDGDPVTADDVIGLVEVM
                     KSFHEVKAGVAGKAIRFLVEDADAIMAGQTLAEVDA"
     gene            complement(24648..25454)
                     /locus_tag="VGA15_01035"
     CDS             complement(24648..25454)
                     /locus_tag="VGA15_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEX9586315.1"
                     /translation="MAVTIVDINCDMGEAFGRWRLGDADDSELMKLISSANIAAGFHA
                     GDPNLMDTTVRLAAQHGVGVGVHPGYHDLQGFGRRKVNGSGDEIINDMIYQVGALREF
                     ARRHGASLQHVKPHGALYMELAVNEALSKAFVEFMRALAPDAFIFCMSVSETYRIARE
                     AGHPVIREFYADRDYDRSGSIVFTRGAGKLDPRTIADKVVRACREGRVRTVEGDDIDI
                     EFDSICFHSDTPGSLEIGRTMRAALVANGVRIAPPSEVLARRAEAFRKPQ"
     gene            25752..26657
                     /locus_tag="VGA15_01040"
     CDS             25752..26657
                     /locus_tag="VGA15_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9586316.1"
                     /translation="MSISLRQIRYFVSTAEHGQVSQAALDLAISQSAITTAIQQIERT
                     IGADLFTRSQQGMELTPAGQQFLFHAYDIINKVAEATSPSIPANDLEGRLSVAATYTV
                     IGYFLPNHLEKMKRGLPKLDIQLFELNREAIEEGLLANRYDIAVLLTSNVLNPDLSTE
                     TLLSSRRRLWVPSKHPLLSESSVGLKEISEQPYVMLTVDEAAHTSLKYWSNTPYQPSV
                     TLRTSSVEAVRSMVANGLGVTILSDMVLRPWSLEGKRIETIVPRNPIPPMDVGLAWRR
                     NAHFTPVMEAFRSYFRQTFFIPTVR"
     gene            complement(26822..27007)
                     /locus_tag="VGA15_01045"
     CDS             complement(26822..27007)
                     /locus_tag="VGA15_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586317.1"
                     /translation="MAKKAKKTVLRREYTRADVKELRAHSKAKTPVAKIAKLTKRTEG
                     SLRQKALKLGIRLGHRR"
     gene            complement(27188..27511)
                     /locus_tag="VGA15_01050"
     CDS             complement(27188..27511)
                     /locus_tag="VGA15_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586318.1"
                     /translation="MDKSKIVNHTVYVIIDRLEPLDENTGHCHAELCAGFSIVADLPI
                     EAFEVRPLTSFRHGAIQHARQFFVGHPDLRHGCPLEDLIDAACAKPRRAVLGTDLGTN
                     ILSLS"
     gene            27526..28029
                     /locus_tag="VGA15_01055"
     CDS             27526..28029
                     /locus_tag="VGA15_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX9586319.1"
                     /translation="MRRPIRTLDVTTAPLASIGLNFATEQPSEAKAVIERSLAAGDRL
                     LERGSFEAGDLTIYRGINGDFHDTVLAASRNRMLGEMIRTCHRVPVSSSRNIVAFEHR
                     DVRRRHDDHHRIHEAIIAGEPWRAEWLMREHVSSVKGSLIRALAARNADQADQTDRPS
                     TWFWRRY"
     gene            complement(28060..28740)
                     /locus_tag="VGA15_01060"
     CDS             complement(28060..28740)
                     /locus_tag="VGA15_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9586320.1"
                     /translation="MADQSAATVRRPRADAVRNRERVLEAAKAVFSAGGPDASLEAVA
                     KRAGVGIGTLYRHFPTREALFEAVYRREVQQLGDLAEQLKSEAEPVDALRRWLRSNVE
                     FVATKKGMSAALALAVHGSSELYAYSFDRLTKAVGALLDRAVAAGEIRSDISPEDLLR
                     ALVGMCYMHDQPGWQKSVLRLLDVFVDGLRVQANVDRETTLVPRRRDATARLKGKPGR
                     TGSRKSRK"
     gene            28998..29495
                     /locus_tag="VGA15_01065"
     CDS             28998..29495
                     /locus_tag="VGA15_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9586321.1"
                     /translation="MNLSISLTVNGVRRDVELGDPRVTLLDLLRERLHLTGTKKGCDR
                     GQCGACTILVDGGRINSCLALALSHDGADILTIEGVSRGDQLHPVQAAFIAHDGFQCG
                     FCTPGQIMSAIGLIGEGQAGDDPERVREGMSGNLCRCGAYSGITDAVLDVQQSLIEAD
                     QRRSA"
     gene            29492..30550
                     /locus_tag="VGA15_01070"
     CDS             29492..30550
                     /locus_tag="VGA15_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9586322.1"
                     /translation="MKTFDYFRPATISDAVAAAAEPGAAYLAAGTNLLDLMKGGVTGP
                     SRLVDVTRLPGLDRIERLPDGGIRIGALVRNADLAHDPDFAKSYPAIAEALLSGASAQ
                     LRNAATVGGNLLQRTRCGYFYDVASACNKREPGAGCEARGGENRLHAVLGWSEACIAT
                     HPSDFCVPLVAFDAVVEIKGSNGKREVAVEALHCLPGDTPERETVLEPGDLIVALRLP
                     GQARAFSAHARYLKVRERTSYAFAVVSAAAGLRIEQGTIRDARLALGGVAAKPWRARA
                     AEEMLAGSRPGPDAFRRAAKAALADAKPSGDNQFKIELAQRILVRALTLAAAGTPQRI
                     PALPASPFSSVSGALHDA"
     gene            30540..32810
                     /locus_tag="VGA15_01075"
     CDS             30540..32810
                     /locus_tag="VGA15_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9586323.1"
                     /translation="MTPEISLTQAPAHARHGSNIGQPLTRRDGVLKVKGEARYAADNH
                     PPGMLHAVLAVSGIARGRVSFLDVQAARSHPGVVDVMTPANRPPLAEDPDAKTNPFMF
                     RLDLLQNDQVRYANQSIAVVIAETLEAATEGAALLSPRYEAQPARVGLDAGESFVPSA
                     VGIGNPSEVRLGDVEAGLAAASKRIDATYETPAQYHNAMEPHAIVAAWDGDTLSIDTP
                     SQGLAMAQGRIAGLFGISPEKIHIRSPFLGGGFGSKGFISGPQVLGILAARLVRRPVK
                     LVLRREHMYGPVGHRAPTRQRLRIGTDRDGLLTAIDHHAKTVSSTFDDFFEPAADASH
                     TLYASPAIATSHEAVRIDTGTPLFMRAPGEATGSIALESAIDEAAWACGIDPLTFRIK
                     NYAEVEPISGKPFSSKALRECYAQGAERFGWSRRPLAPRQLRDEAGLLVGWGIGTATF
                     PALMFQAEARAVVRSDGSGLMETGAHDMGQGAWTALAQIAADGLGLELEQMEFRAGTS
                     DLPDAGIAGGSAHTATAGMAIHNAGAAVIGKLADLATGDERSPLFGAGNAGVLARDGR
                     LFRRDDETRSESYADILGRAGLREIEARGKGAADPAAQSTYAMHAHGAVFAEVKVDPD
                     LGQIRVTRMVGAFAAGRVINPRLVRSQIFGGMIWGVSFALHEHAVMDRRSGRILNANL
                     ADYHVPVNADVPGLEALMIDEHDPHVNALGIKGVGEIGVTGSAGAVANAVWHATGIRV
                     RRFPIRIDDLVTPASH"
     gene            complement(32838..>33078)
                     /locus_tag="VGA15_01080"
     CDS             complement(32838..>33078)
                     /locus_tag="VGA15_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX9586324.1"
                     /translation="FTAIGVFLMLVSIGLAIPVIVTYLEQGIVPRLPTAVLSMGLMIL
                     AVLSVSSGLVLDTVTRGRREIKLLAYLSQPTMDRH"
BASE COUNT         6488 a        10561 c        10028 g         6001 t
ORIGIN      
        1 ccagcctctt tcgccagcgt atcgatgctg gcttcgccgc cggcattgga acggatatat
       61 tccgccgcgc gcctgatccg ccgatcgcat gccgacacgt tcaattcgcg gatcgatctc
      121 ggaaagctct tccagggcgt gaagcgctcg atcaccgcga tcatcagttc cgacaacaac
      181 gcttcatatt cggcctggca ttcgggatcc gccatcatct cggacgcgag atccatcgtg
      241 agttttcgga tgcccggcga tacctcgcca tggggagttc caaaaaaccc gggcgcgccg
      301 cttgccgccc atcccggccg aaaggccttc agccatccag gttcgacata cagagcaagg
      361 ataagcgtcc gtggacgcgc cggatcgtga acataggaat gcggctgcca gccgtcgacg
      421 agcaccgcgg cggaatcggt cagggcatga atgcggttgc cgaccacgaa ttgggtatcc
      481 gcgcctgcca ccttgaacag gacatgacaa tgcgggtgcg catggcggac cagcgagcaa
      541 tccatatcga gcagggcgac cctgccgaaa ttgccgtgta cgatccgcag agccgtcgac
      601 attcttgcct ccagtcaggc gttagacttc cagggaatga gccgccgctc gatctcatcg
      661 gcagcgaccg ccagacgatg ccgacaatcg cgatgaccgg cgggccgaca tacccagggc
      721 cgacctgcat ggtgtcgacc gtcagaacct gccaagcgtt ttcgaattat gacgccgacg
      781 ccatcattgg cgcaaccgtt tccgagatca tgaccagaaa aagccccagc tttattcccg
      841 ccacgatcgg agccagctca ggactaccac ggcacgatgg cggtgcggtc gcttgccatc
      901 tcgcataatc tgcggaaacc tctccaacaa atcacataaa ggcgtcgcga aaccacatca
      961 tgaccagcac agagacgatc agctttgaac tcgaccagcc cgctttgcag gcgcgccgca
     1021 acgccaaatg gaaccagtac gatgcggata tattgccggc ctttgtcgcc gacatggatt
     1081 tttcggttgc cgccccgatc caggccgcga ttgagcggat cgtccgagac cgcgactatg
     1141 gctatgcgtt gcgcaacggc gaaaagccgg accggctggt cgccagtgcg ttcgcccagc
     1201 ggatgaaatc ccgcttcgga tgggagctct cgcccgatct tgtgctgccg ctcgcggatc
     1261 tggtgcaagg cacctatgcg ccgatcctgg cattctccga tcccggcgac ggcgtcgtac
     1321 tgcaaacccc gaactatccg ccgttccgcg acgccatcag ctcgacggaa cgcgagcttc
     1381 ttgcgctgcc gatgcgcgac gacgggaccc gcttcgtctt cgacatgaac gagcttgaaa
     1441 agctggtcga caagcgaacc cgtatcttca tactctgcaa tccgcaaaat ccgacagggc
     1501 gggtgttttc ccgtgacgaa ctgctcgcat tgggccgatt tgccatcgaa cacgatctcg
     1561 tcgtcatttc agatgagata cattccgacc tggtgttccc ggggctgcag cacattccct
     1621 ttgcatcgct cgggccggaa attgccgccc gcaccgttac cctcaactcg gcgaccaaga
     1681 gtttcaatat tcccggcctt cgctgcgcgc tcatggcctt cggctccgaa gacttgcgcc
     1741 atcggtttca caaacgcatc ccgcagagac tgacaggttc ggccaatatc gtcggcgtcg
     1801 acgccacggt cgcggcctgg agcgaatgcc agccctggct cgacgcggtg atggagcatc
     1861 tgctcgaggc gcggaaccgg gtcaaggatg tgctcgcggc ggaagtgccg gaaatccgct
     1921 tccatgcgcc ggaggcgacc tatctcgcct ggctcgattg cagcaaactc aggctttcga
     1981 ccccggcgtt ccagtttttt ctcaacaatg cgcggatcgg atttagtgcc ggcgagacgt
     2041 ttgatccgga ttgcgcgcag tttgtcaggt tcaacttcgc aacgtcgatg ccgatcctgg
     2101 acgatatcct cgatcgcttc gtcgccgcga cacggcagaa cgaccgatga tgccgaatat
     2161 cgccgtttcc tgttcgaacc gatggcttac cggccaaacg catagccctg catatagcgc
     2221 gggttcgcgg cggccctgag gatgcggccg tccttcgaga ctgccgaaat tcttcccaaa
     2281 gaccaggcgt cgacgatctc gacccgatgt ccgcggccaa ccagatcctt cagaacggcg
     2341 tcgctgaaac gcgattccag cgtcagcgag cccggcttcg cctgacgggg aaagaacgaa
     2401 ctcggcatgt ggtcattgtg gaacatcggt gcgtcgatcg cactctgcaa gttcatgcgg
     2461 tgatgcgcat ggcgcagaaa aaacaccgag ctccactgat cctgctgatc tccgccgggt
     2521 gtcccaaagc tgccgtaagg tttaccgtcg cgggtgagca gggataccga aagcgtcgtt
     2581 cgcggtcgct tgcgcggctc cagcgaggac gccaggccgg gtttcagcca gaacatctgc
     2641 gcgcgcgtac ccagcgcaaa tcccagtccg gggatcgctg gcgagctttg caaccagccg
     2701 ccactcggcg tggcggagac gatattgcca tgcctgtcga tcacgtcgag gtgcaccgta
     2761 tcgccgcgca ccgatccgcg cgggccgacc gtcggttcgc cggcgcctaa tgccacggcc
     2821 ggctcgccgg tccgggcaac gacgtagggg acttcacggc cgtagcctgc gatcgagccg
     2881 ggccggaatt caagcgatgc ggtgttgccg accagacggc ggcgctgatc ggcgtattcg
     2941 ctgctgagca acctcgccat cgggacgtcg acaaaatcgg gatcgccgta atatttttcg
     3001 cgatccgcga aggcgagctt cgaacattcg gtcacgaggt gaacgaattc gccgcccaca
     3061 ggatcgagat cggaaagatc aaaacccttc aacagcgcga gctgttgcag caataccggt
     3121 ccctgcgtcc agggaccggg tttaaggacc tcgaatgcac cgtagctcag cgacaacggc
     3181 tcttcgtacg tcgcctgcca cgtcgataaa tcctgtcccg tcagcaggcc ggcatgacgc
     3241 cgaccggacg tatccatcgc gggtgtacga acgaatttgt cgatcgcctc cgccacaaag
     3301 ccctcgtaga aggctttgcg cgcggcttcg atcttctgct cgcggttgcc cggcgtatcc
     3361 cgcgatatca gaacgatgcg ctcgtaggtc gatgcgaggg ccggattggt gaacaatgtt
     3421 cctgccgacg gaacgccgtt tcgcagatag agctcagccg acgtcggcca ttcggttcgg
     3481 aacagcggcg cgaccatctc gatcgccgcc gcaattctcg gcaacatgac gtatccgtca
     3541 cgggcgtaag tgatcgcggt ctcaagcacg tcggccacat gcatggtgcc gtagtcgcga
     3601 agcagcgtca tccaggctcc gaacgagcct gggacgcaag ccggaaggaa gccgctgccg
     3661 ggaatgatgt cgaggtcgag ctccgcgaaa cgctcgatcg acgcggctgc gggagcaacc
     3721 ccttgtccgc aaagcacccg ggcccgacca gcgcgcgcat cgtgaaatat gatcggcacc
     3781 tcaccgccac aaccgttcaa atgaggctca accacttgta acgcgaagcc gatggcaact
     3841 gcggcatcga aggcattgcc ccccttctcg aggatacgca tgccggcggc ggtcccgagc
     3901 caatgcgtcg acgccacgac gccgaaggcg ccgacgattt ccggccttgt cgtaaacgcg
     3961 gattcgccgg cgctcggccc ataactcaat ggcgtgtttc ccagcgccaa actgtccgac
     4021 atgctcgcta cctcgtactc aaaatccgcc tgaccctaac gatgccgtca ggccaactca
     4081 cggcgcgcct cgtcaagcaa cagccagaca tagggcgcga aggaacgcca gacatcgata
     4141 tgaaacgtgt cgggttctgg ccgcgacagc aggatcgtcg ccttacccaa aatcgtccgc
     4201 gtacacatgc cgaccggaaa ggcctgatgc gacaggtcga gaggacagcc gtggttgagc
     4261 acatagtcgc tcatgcgtcc cgacaaggcg aaagcatcgg accgatgact gacatcgacg
     4321 agcgaacagg aatagccaag cagcgcctct tccatgaccg cgaacagcac ggaagcctgt
     4381 tcgcctgtgg cctgaagcag ccattcgtcc ggaccgagcc agaatgcggc gcggttgccg
     4441 ttcgcggcaa accggcaggc cgtctgcgga aacgacaccc cgaatgcttc gccggccgcc
     4501 gggttcgagg agggacggcc gcggaacgac agcttcgcgg cgtcagggag cacagcaagc
     4561 atcgccgtcg gcacgtgcgc cgcgctaccg gccaattcgg ccaatgaatg cgatcgtcgg
     4621 gcggacagat caagcatcga gacgctctcc cttcgggtcg tagaaaacag gcgcaacgac
     4681 ctcgaccgca atggggccgc tcggcatcgg cagcgacagg cgcgcgccga tgcgcgcgcg
     4741 tccgccacga accagcgcga gcgcgatcga acgatccagc gcggcgctcc aacaggacga
     4801 cgtcacgtga ccgatcatgg tcatcggcac cggctcggcc ggatcggcga cgatctgcgc
     4861 gccttcttcc agcagcgttt tcggatcgac cgtgagcagg ccgaccagtt gcttgcgatc
     4921 gcttcgcgtc atggccggac ggaccagcga acgttttccc acgaaatcct ttttcgattt
     4981 acccactgcc caattgaggc ccgcgtcatc cggcgtcacc gtgccgtcgg tttcctggcc
     5041 gacaatgatg tagcccttct ccgcgcgcag cacgtgcatc gcctcggtgc cgtagggcgt
     5101 aatgccatgg gatcgcccct ccccgatgat ggcctcccag accgctcggc cgtatccctg
     5161 gggaatgttc acttcgaagc cgagttcgcc ggtgaaggac acccggaaca gccgtgtcgg
     5221 cacgccgcaa attttgcctt cgcggaggct catgtgcggc atcgcctgcc gcgaaaggtc
     5281 gatgtcttgc acgagcggct ccagaaactt gcgggcgttc ggtccctgca ccgcgatcac
     5341 agcccattgc tcggtggtcg acgtcagcca gacctccagg tcgggccact cggtctgcag
     5401 gtagtcctcc atcatggcca gcacccgggc ggccccaccc gtcgtggtgg tgacatggaa
     5461 tcgatccggc gcgatgcggc cgacaacgcc gtcatccatg ataaacccgt cctcgcgcag
     5521 catcacgccg tagcgcagcc ggcccggttc gagcgtggtc cacgcattgg tgtacatgcg
     5581 attcatgaac tcggcggcgt ccttgcctac cacttcgatc ttgccgagcg tcgaggcatc
     5641 gaaaatgccg accgactgcc gcgtcgcctt gcattcgcgc gtcaccgcgg catgcatgtc
     5701 ctcgcccgcg cgcggaaaat agcgcgcccg tttccaaagg ctgacgtcct cgaaaaccgc
     5761 gccattttcc gccgcccagc cgtggatcga ggtcttgcgt tccggatcga acaggccgcc
     5821 gcgcgactgt cccgcgaaca cgccaaacgt ggttggcgta tagggcggac ggaacgtggt
     5881 gaggccgatg ctgggcaccg gcaggttcag cgcatcggaa accacgccaa gggcgttcat
     5941 gttcgaggtc ttgccctggt ccgtcgccat gcccgtggtg gtatagcgct tgacgtgctc
     6001 gatggagcga aagccctccc gggtggcgag cgcaatgtcc ttcgccgtga cgtcgttctg
     6061 gaaatcgatc caggcacggg tcttcaacgg gtttcggccg tgaggcgtcg cgccgatgaa
     6121 actagcgatt ccaacatcgt cagacttgac ggcgaagcgg tgctcgatcg tcttgttcgc
     6181 gcccgccgcc acggccgcgt tcatgccgcc ggcaaaccca tcatgcaaaa ccttctccag
     6241 atcgtagacg ccgcgacatc cgccgaccga ccgctcgcgt tcgacggagg tgcccggaac
     6301 ataagcttgc aaagcgtcgt cgtagacgag cttgccgcgg gattgcgaga acagatgaac
     6361 gctgggcgta aagccgccgg acatcagcac cagatcgcac gcgacggtct cggccttgcc
     6421 aagacctccg ccggtagccg cgcgggcgag gcttgcggac ctgacgcgca ggcgcccgcg
     6481 cgttgccgtg acgacggtcg acggacgcac gtcgatcccg gctgcgcggg cacgcttgac
     6541 ccagaggctg ccgggcgcgg tcctcagatc cgcgatggcg acaatggcaa cgccggcggc
     6601 cgtcagatcg atcgcggcgc gataggcgct atcgcaggcg gtaaagacca acgccttgtc
     6661 gccgggccgg acaccgtacc ggttcgcgta agcgcgggcg gcttccgcca tcatcacacc
     6721 cggccgatca ttgtcgggga ataccatggg ccgttcgatc gcaccggtgg ccagcacgac
     6781 ttcttttgcc cgcacctgcc agagccgctc gcgcggctgc ttcggatcgg gtgaggcgca
     6841 atggtcggtg acgcgctggc acagcccgag gaaattgtgc ggataccagc cgaaagccgt
     6901 ggtgcgtggc atcagggtta cgttgcgact taaggccaat tcgccgagcg tcgtcatgac
     6961 ccaatcggct gccggtttgc cgtcgatgac ggcggtggtc tcggtcaaca gcgaaccgcc
     7021 catttcggcc tgctcgtcgc agacgatgac ttttgcgccc gacctggccg cggccagcgc
     7081 cgcggcgaga ccggcaggcc ctgcgccgac gaccagcacg tcgcaatgga caaagacctg
     7141 cgtatagcgg tcggcatcgg gcctgaccgg cgcgcggccg agcccggcgg cacgccgaat
     7201 gagcggctcg tataatctgt tccaggccgc cttcggccac atgaacgtct tgtagtagaa
     7261 gccggccggc aagaacgacg cgacggattc gctggccgcg ccgacatcga acttcagcga
     7321 cggccagcgg ttctggcttt ccgccaccag accgtcgtga atttcaatct gcgtcgcacg
     7381 caaattgggg gtgtagcgcg ccgcatcggc gccgacgccg accagcgcgt tcggctcttc
     7441 cgatccggcc gtcatgatgc cgcgcggccg gtgatatttg aatgagcgcc cgaccagatg
     7501 catgccgttg gcaatcagcg ctgacgccag cgtatccccg gcaaagccat cgtagcgctt
     7561 tccgtcgaac gtgaagcgaa ccggccggct gcgatcgatg cgcccgcccg cttcgaggcg
     7621 atacgcggcg gtcatcgctt ggtctctttg ctcgcggcaa cgggtggctg ctcgcccgcc
     7681 ggataggtcg ccgcgatcgt gtcggttacg gtgtcacggc gcgcattgaa gaaacgaccg
     7741 caaccgtgca ggtgccgcca gcgttcggca tgcattccct tcggattcct tcgcatgtag
     7801 aggaagttcg cccaggcctc atcgtcgagg ctcatgggat cggcgggacg ggcgatatgg
     7861 gcctcgccgc cataggcgaa ttcaagctcc gagcgggggc cgcaatatgg acagcagata
     7921 agcagcatga tcgcgacgac ctcggctcag tgggctacgg cggcggcggc ggcttcgtcg
     7981 atcaggacgc cgtcccggaa acgctcgatg ctgaacggcg cattgatcgg atgcggttcg
     8041 tccctggcga tggtccaggc aaaaacatcg ccggagcccg gcgtggcctt gaaacccccg
     8101 gtgccccatc cgcagttcag gtaaagtccg gcgacagggg tcttggcaat gatcggggaa
     8161 cggtccgggg ttacgtcgac gacaccgccc caattgcgca acattcgcag ccgccggaac
     8221 atcgggaaga gttcgcagat cgcatcgatt gtgtggttgg cgatgtggag cgcgccgcgc
     8281 tgggaatagg atgtgtaggc atccgtgccg gcaccgatca ccatctcgcc cttgtccgat
     8341 tgcgaaatgt aggcgtggat ggtgttggac atcaccacgc aaggaagcgc cggcttgacc
     8401 ggctcggaga ccagcgcctg caacggaaaa ctctcgagcg gcaatctgat gcctgccata
     8461 ttcgccacca tgctggtatt gccggcggca acgatcccga tttttctcgc tgcaataaag
     8521 ccgcgggacg tctcgacgcc ttcgaccgcg ccggaggcgc cacgccgtat ccccgttacc
     8581 tcgcaattct cgaggatatc gacgccgcgc gcgtccgcgg ctctggcata tccccacgcc
     8641 acggcgtcat gtcgcgccgt tccgcctcgc cgctgcaaag cgccgcccaa aataggatag
     8701 cgcatggcgc cgcccacatt cagcggcggg cagaagtcct tgcactgctg cggcgacaac
     8761 cattcattgt cgataccgtt caggcgattg gcatggacgt gtcgcctgga gctctggacg
     8821 tcatggacgg tgtgcgccag catcaaaacg ccgcgcggcg aatacatcac gttgtagttg
     8881 agcgcctgcg aaaggccctc ccacaatttc agtgcatgtt cgtaaattct ggcgctttcg
     8941 tcgaacaggt agttcgagcg gatgatcgtg gtattgcgtc cggtgttgcc gccgccgagc
     9001 cagccacgct ccaccaccgc gacgttcgat atgccgtatc gttccgccag ataataggct
     9061 gtcgcaagac catgaccgcc gccgccgatg atgaccacgt cgtagtgcgg cctcggttcg
     9121 ggcgaacgcc attgccgttg ccatttggca tggccatcaa gaccgccgcg aaacagggaa
     9181 agcgctgaga atttctgcat cggatccgtg cgtgttcgag ctcgacacta ccgcttgcct
     9241 gccatgcccg ccagcattgc gcccgcctga ccggttcgcg ctctagtgca gcggcgcttt
     9301 cgcggatgag ccgacaagct gggcccagcg tctgacgagg taattgtgct cgtccatcgt
     9361 ggtgttccaa accgcgatgc ggctggcgcg ctgccgatag gcgcccccgc ttcggacctc
     9421 gccgcggccg atggtccgtt cgccgtcggg gccgggcaag tcctccgccg ccggctcgcc
     9481 ctcataccag taattccatt cggccaaaga aaggtgttgg cgaaccctct cggggatcga
     9541 catgtagtag ccctgccgcg ccaccaccgc gccggcccac ccgtcgagcc accaattgag
     9601 atactcatag gcgatatcga gcgtgcgacc gctcagatgg cgcgccaggc aaaggccgcc
     9661 gtgccaggcc cgatatcctt cctgcggcac cgcctcgcga acgggaaaat cacgccggcg
     9721 cagcaggctt actcccggtg accacatgct ctgcatgatc gattgcccgg acagcatcaa
     9781 atcggcggct tcgctcgcgg tcgtccagaa gccgcagaaa tagccggcgc gctttcgctc
     9841 cgcgagaaac tcgatcaggc agtcgatttc cgccgtcgac atgttgccga tatcgtcgaa
     9901 atgcacttcg cctctggcct gcgccgcgag cgctgcatcg aacacgccga tggcgggctc
     9961 atcgaccagg gcgacccggc cggcccagcg atcgtcgagc agcgcgcccc aggaggcgct
    10021 cggctccaga tggccgttga cgagaccggc gttgtaggcg aacgagtcga gattgtgcac
    10081 cgtcggcagc atgctgatgc gcccggaagg cgcggagccg agcgagcggt tcggctgggc
    10141 gaacaacttg gtgaccggcg catcgccgcg gccgatggcg gcgccggaat gaatgcgtcc
    10201 cgtcttggtg agatcgctga cttcgttcca gcgcacgatc cgcttgaggt cgatcgactg
    10261 aatggcgcgc cagaaccaga caatatcgag attgtggaag cactggtcgt agacgtcata
    10321 ggtctccggc tggatcgcgg ccttgcgctg ggcgctgatg aaatccagat tctcgaacac
    10381 cagcgtgatg ccgagatcgg cttcggcgtg gaggcgaatg gactcaagca gcgtgatctc
    10441 ggtcccgagc acgcgcagga ccggtcgtcc ctgaagatgc agcgcggggg attccggcgc
    10501 cccggctttc ggcatggaca ttggcggctc gcgacgatcg gagtttttga ggaagccgcc
    10561 gggagatcgt tacgcggcga ccgacatgtt cagacgattg aatatcgaga aagagcgtcg
    10621 aaccaagtca ggctcgatcc cttccacgat aaagactaat cgagaccgat gatcctcgct
    10681 cggccaccgg ctcagatggg tcggcgcatg cacgagatgt tgcaccccat ggacggcgac
    10741 cggcagctct tcgccctcga gcgccaggat ccccttgacg cgcaggatcc gcgcgccatg
    10801 gcggttgagc agcatggtca accaaacccc gaaagccgtc caatcgagcg gcgcatcgac
    10861 gatgatcgag aaggcgcgaa cgcccgcatg agcggccatt gccatcgaag gctccgcgat
    10921 ggccttgaac cagccgcttc cgccagcttg caaaagagca tccgcgtcca ggggagcgtc
    10981 ggcggcggac agacgcggag cggaaggatt gatggcgtca agccgcgcgg ccagccgtgc
    11041 cgtgacggcc tcctcggcaa tatcgctctt ggtcaggacg atcgcatcgg cgaccgccac
    11101 ctgacgcacc gattcgatat agcggtcgag ctgtccgaac ccgttgacgg cgtcgaccgt
    11161 cgtgacgacc ccggcggcgc gaaagtggtg tcgcagaacc gggtcggcct tgagcgtcga
    11221 cagaacggga aatggatcgg caagtccggt gctttcgatc accagccgtc ggaacggcgg
    11281 cacgagaccc cgctcgcgcc gggaatgcag atcgcgcatc gttgtcgcca gttcgccgcg
    11341 aacggtgcag catacgcaac ccgaactcaa gagcaccatt tggtcgtcca gacgctcgac
    11401 gaggaggtgg tcgatcccga cttcgccgag ctcattgatg atcacggcgg tgtccttgag
    11461 ggcgggatcg gccagaagcc gtcgcagcaa cgtcgtcttg cccgagccca gaaaccccgt
    11521 gagcagcgtt acccgcgtga aggccggaat gtcctcaacc atggtcggca tccagcgctt
    11581 cgagaaggtc gggcgcgagg ggatcgcaaa ttcggccgga cagcttttcc gccgccggcc
    11641 agagatgacc aaggtcctcg acgatctcgc tcttcccggt gagcgtcgag agcagaaagg
    11701 atgtgccctg ccagtcggaa agccgcatac cgtaaagtgc cagcacgcga ttggcgaggc
    11761 ggacacgatc cgcgcgttcg cgccggcgcc gaactggatc ggcattgcgc gtgtagacgc
    11821 cttcccgcgc cgccgcatcc gcccaatgat cattgcctcc gaggctgccg cacagcgagc
    11881 acatggtcgt ctctcctgac cacctggaaa gccgctactg ctttcgcggc gaacgcctgg
    11941 cgaaatcgtc tgccatctcg ttcatggtaa caaatctcac gccggggtgc gaggcgatat
    12001 gcttgtagag ccgctccagc atcatgagca cctgaggccg gcccgagacg tcagggtgaa
    12061 tcgtcatcgg gaacacggcg tagtcgtttt cgcgatagac ccaatcgaac tgatcgcgcc
    12121 acatctcctc aagatgccgc ggattgacga agccgtggct gttcgggctc ttcttgatga
    12181 acatcatcgg cggcaggtca tcgagatacc aattcgccgg gatctcgatc agatcggtct
    12241 cgacgccgcg tttgagcgga gtcatccagg ccgatggatt tttcgagtag tcgatcttgg
    12301 tccagctgtc gccaacccga acgtaatagg gatggaaatc attatgcatc agcgaatgat
    12361 cgtatttgat gccgcgttcg agcaaaagtt cgttggtcac ggtggagaat tcccaccatg
    12421 gcgcgacata gccggttggc cgcttgccgg acagccgggt cacgagatcg atgcatttgt
    12481 cgaggatgat ggtttcctgt tcgcgggtca tcgcgatcgg gttttcgtgg ctgtagccgt
    12541 gaatgccgat ctcatggccg gcgtccgcga ccgcctgcat ttgcttcggg aacgtctcga
    12601 tggagtggcc cggaatgaac catgtggtct tgatgcccag gcgttcgaac agttggagca
    12661 cacggacgct gccgacctcg ccggcgaaaa gcccgcgaga aatatcgtcc ggcgaatcct
    12721 cgccgccgta ggaaccgagc catccggcca ccgcatcgac atcgacacca aatccgacaa
    12781 gaatttcctt cgccatatga ttttcctttc gtgaggtttt gcggctaggt ccgcggcatt
    12841 caggccgcga cgatggttgc attgtgtgga tgccaggcga tccacacgct ggatgaagga
    12901 acgagatcgt tgcgccgata tttatcgacg tgcgcttcaa gaatgaccgt gctgccgccg
    12961 gccagcgtaa tcgccatctg cacgaggtga ccgacgacgt cggcccgctc catcacgccg
    13021 ggcagcacgt tgccctggta ttgcccggcc aacgcctcgc gcatccctcc cgccgacgga
    13081 tatatttcga gcgactccga aggcacgacg atcttgaccg cttgccccac ggccgcgcga
    13141 tcgtccggcg tgccgacgaa ttcgccgaat gccgtcgaga gcgtcgcgcg gtcggcttcg
    13201 accttgatca acgagccgtc gatgagcgta ttgcggccga cgaactgggc gacgaatggc
    13261 gtctttggcc tggtatacaa atccagcggg ctcgatacct gctcgatctt tccctggttc
    13321 atgaccacga ttcgatcgga cagcgtcagc gcttccgatt gcgcatgggt cacgaaaaca
    13381 aacgtgatcc cgaggtttcg ttgcagcagc ttcagttcag cttgaatggt cttgcgcaga
    13441 ttggcgtcaa gcgcgcccag cggctcgtcc agcaacagaa tgtccggttc gacgacgaga
    13501 ccccgtgcca gcgccacgcg ttgccgctgg ccgccggaca gtttgtgggt aagtgaatcc
    13561 gccacgacag acaggccgaa cttttccagg atgccatcga ccttggcgtt acgttcggcc
    13621 ttcggcatgc ccttgacgtc aaggccataa ccgacattcc cgcgcactga catgtgtgga
    13681 aacagcgcgt agttctgaaa gatcatcgag gtcggccgac gctcgggtgg cacattattg
    13741 atccgtcgtc ccctgatcag gatgtcgccg ctggtaattt cctcaaaccc cgcgatcatg
    13801 cgcagcgtgg tggtcttgcc gcagccggac ggtccgagaa aggcgatgaa ctcgcctttc
    13861 ttgacatcga gattgaaatc cacgaccgcg ggcgtcccgt tctcatagac tttgcggaca
    13921 tgtctgagct gaagatcaaa atccacgaat ggcccattcc atacgatgcg atcacgcgac
    13981 aagagaggaa gggccggcgc agagcgcgcc gacccgccat ggttcggctc aggcgctgag
    14041 gaactcgtcc catttctgga tcacgaggtc gtattcgtcc ggccactgat gccagtaggc
    14101 gacgttggcg gcgcgcgttt ccagcgaacc cccatcgcga aggtcgccct ccttgatgcc
    14161 gttctcgctc ttgcccttcc agggcttgcc ctcataccag aaggcatatt tgtcagccgg
    14221 catggcgttc ttgatgttgg tcgatggcga ataatagcct tgctccgaga cgatgatgcc
    14281 gggcgttccg gacaaccaat aatcggcata ggcgtagacc gcctcctggt tcggagagcc
    14341 gacgatcatg gacggcccga tcgaccaggc gcgatagcct tcctttggaa cggcatattt
    14401 cgcaggcttg ccctgcgcct tgaccgccat cacggcgggc tgccaggcgt cgcagacgac
    14461 catttcgccc gatgccatca aattcacgag ttcgccgaag tcaccccaga gcgcccggaa
    14521 ctggccttcc ttcttcttcg agaccaggaa cttcatggcg tcttcgatct gttttttcga
    14581 cgcattgccc gggttcttga cgtcagacag gccgagcgtg ttcatcgcca tgatggcctg
    14641 gccgagcgca atcaaaggat cggtgttgag acccgatttg cctttccact tcttgtcaaa
    14701 gatggcggtc cagctgttgg cttcctcatc gctcagcacc gaaggattgt agccgatgga
    14761 atcgtaatta tagacggccg gaaccatcca caacttggtc tgcgcgtcgt cggcccagat
    14821 ctggccggtg atctgcgcct tcttgtcgaa cttcgcttcc gacttggtga acgtgtcgcg
    14881 aatattcgcc cagttcttga gcgaagcggc cgggatcggc tcgatcttgt tggtctgggt
    14941 caccgcgggc aatcgttcgc caatggtttc ccaacaatcg aaatccttcg aacccgaaag
    15001 gatcttggtc tgcgcgtccg ggaaagtcgc cgccgtgccg gcggtcgagc cgacgccgga
    15061 aacagccttg tagtccgcca ggatgcgatc ctgaacggtc acggagaggc ctatcgtgcg
    15121 cagctgttgc ttcgcgacat cggaagcggc cgcataagcg gtccgcgacg agacaaaggg
    15181 cgtgccgcca ccgagcgcaa gcgcgatcgc gccggccgag ccctggaaca atttgcgtcg
    15241 cgtgagttcg agcttgtcaa ccattggatt acctccatct gaacagtttc aatcgttcgc
    15301 caaaacggtc gtcttgagcc tctggcctaa tacctgccga ccaggagacc gccgtcgacc
    15361 accagggttt ggcccgtcat gtaacgagcc gccgaggatg ccaggaacag aatcacatcg
    15421 gcgatttcat ccggttcgcc gatacgtccc attggaatga attcgccggc cttctcggcg
    15481 ccggcaggtc cgagcgaatg cttctcggac agaagctgag cggtccggat gtaaccgggc
    15541 gcgatgccgt tcacccggat cccgtttcgc gcaagttcga cggcgaggcc gcggatcagc
    15601 ccgacgacac cggccttggc cgacgaataa tgcacatgct cgtcccagcc ataggcgacg
    15661 cccatgatcg acgaaataca aatgatcgcg cccgccttgc gggctttcat gccgggaagt
    15721 gctgagcgga taaccctgag cattcccttg agatcgatat cgaacgtttc atcccatttt
    15781 tcgtccgaca ggtcttcgag caggaccttg tgcgcaattc cggcattggc gacgacgcaa
    15841 tccagcgcgc caaaccgggc gcacaaatcc tgaaccaaac gatcgacatc agccgtggat
    15901 cgaacattca gcaaatggaa ttccgccgaa ccacccgccg aggcgatgtc ggcggcgacc
    15961 gcccgcccct cctgctccag cacatcggtt accacgacat gatagccggc tttgccgaag
    16021 gcgaacgccg tggctctgcc gatcccgatg ccgctgccgg tgatcaaaac ggtcggtttg
    16081 gtcgaagcga tcttgccgtc ggtcatagca tcacgtcccc ggaatttggc cccagagtct
    16141 gtccaacata aagactggcc tccggcgagg cgagaaatga gacggtcgcg gcgacttcgt
    16201 cgggctcgcc gaagcggccg agcggaagct ccttcgcctt ggcctcgcgc caatccttcg
    16261 acagcgccat gaccagcggc gtgttgatgg gccccggcgc cacggcattg acccgcacgc
    16321 cctgcgcgct cacctcgcgc gcgagcgatt tcgtcatccc gatgatcgcg gccttggcgg
    16381 cgctgtagtg aacgagctcg acgccgccga tctgtccgag ttgcgacgcg acattgatga
    16441 cgatgcctcg ctctttcgcg agcatcgccg gcagaacggc gcgcgtgcac aggaatgcgc
    16501 cgcgaacgtg aacggcgaac atccggtcaa aatcggcagc cgtcagatcg acaaatctgg
    16561 cctgatgcac gatgcccgca ttgttgacca ggacgctgca atcgccgaac gccgcccggg
    16621 cgaacgctgt gatcgccgca acatcgtcct cgcgcgagac gtcgccggcg atcgccttgg
    16681 caaggcaatt gctggcacag atctcctggg ccaccaattg cgcacgctcg cctgacacgt
    16741 cgttgacgac gacggccatg ccatccctgg ccagccgatg cgcgacagcg cggccaattc
    16801 ccgagccggc gcccgtcaca atcgcgactt cctgcgccat cagctttcct cctgaatggc
    16861 gatccgcctg gcgcggcgga ccgaaagcgt catgaggatc gcgtaaaccg cgagcaaggc
    16921 gaaggagaac agcgtcgtca aaacaccaaa cgcgaaaatg ttcggatgaa tctcgatcga
    16981 gaacgtgccg tagatcgcca gcggcaaggt cagatccggt ccggatgaaa acaacgtccg
    17041 cgaaaattca tcgtaggaaa gtgtgaatgc gaacagcatc gcggacagca cgcccggaaa
    17101 aatcagggga aacgtgacct tctgaaatgt cctgatcggc gagacgccca gcgaccagga
    17161 ggcttcttca accgaggcat cgaagcgatt gaagatcgcg agcatgacca gaaacgcaaa
    17221 cgggaaggta tagacgacat gcagaacgaa agccgttccc caccaggtcc ggtcgatacc
    17281 caggatgttc gcgaccaaag caaggcccag accgacgaga acgcccggca ccatcatgcc
    17341 aaggacaatg agatagaaga tgacgcctga tcccttgaaa tgggtgcgaa aggcctgggc
    17401 cgacatcacg ccgagcaccg tcgacacgac cattgtcatc atcgccagca gcaacgaccg
    17461 tatgagggaa tccctgatcg gcaacggcgc gatgcgcgat ggcggcgcca ggccgaagat
    17521 atcgcggtac caataggtcg accattcgat gatcggaaat tgcggaccgc cgttcggacc
    17581 tgtctgaaac gacaggatcg caagcacaag gaagggaccg taaagaaata cgagaaacag
    17641 agcgacgtag actgccaaaa cgggtttgat gaatgtggcc tgcatgacct caaagctccc
    17701 gcttgagatc gacgatgcgc agcaggcccg cgatgacaat ccccatcagg atcgtcagga
    17761 tgaccccgac cacggaagca aaggcccatt tgagcgagcc gacctgagtg acgataatgt
    17821 tgccgagcat gttcaccttg cggcccgaca gcgcggacgc ggtcgcgaat tcccccagca
    17881 ccattacgga cacgaagatc gacccgacga tgacgccggg catgctcagc ggcagaatga
    17941 tggtgcggaa tatcaggccg aaactcgcgc ccagatcccg tgccgcttcg atcacgttcg
    18001 gatcgattct tcccaacatg aacgcgatcg ggccaaccat gaagacacag tagatctgcg
    18061 tcatcccgat gatcacggat agttccgtga acagcagcgc gtcgatcggc gcggatgtga
    18121 tgtgaagcat ttgcaggatg atgttgatgg cgccctcctt gccgagcatc ggccgccagg
    18181 cgaggacgcg gatcaaaaac gaggtccaga acgggatcac gcaaagcacc agcagcgcgg
    18241 tcgacagcgt cttgtttttg acgaacaggc cgacaaagag cgcggtgggg tagccgacca
    18301 gcaaggtcgt gaacagcacg atcagccaga tccgaacggt ggtccagagc gcgccgatat
    18361 aggtgtcgct ggcaaaaaac gccgcccagc tgcgggtggt caggatcggc tcgatcttga
    18421 acgtggtttg cgtccagaac gacacgtaga cgatcatgag gatgggaacg acgaggaaca
    18481 gcgtcatcca caacacgccc ggagcgatca accaataagc cacacggctc aagccgcgcc
    18541 tgcccgcgac gggcaggctg ggtttatcga tcgcggcgtg caagagaacc gtcatccctg
    18601 gatccatccg ctcgaattga gcaaaggcta atcatccgaa aacaagcgca ctctctgttt
    18661 tccaagtcag cgtgtagtcc gcattgacat ccagattctg catcgcgcga tgactgagat
    18721 ggttttccac ctcgacgatc gatccgctgt gcaactggaa aaagtacatg aacgccgagc
    18781 cggtatattc gctggcaagg tagcgggcgc tgatgctctc ttcatcgggc tcgttggtcg
    18841 cctcgccttc gcgtatcgcg atcatgtcct gtcgtatcgc gtaggacgta tcggcgctgc
    18901 tcgttctcga gccgcctagc ctgaaacgat gatcttcggt gatgaacagg ccggcgtcga
    18961 tttcgccgcg aaacagattg tagcaattca aggatttcgc gacgcgcggc gaagcgggct
    19021 ggttgaatac cacgtcggga gcgtcgaact gatcgacgcg gccacgatcc agcacgacga
    19081 cccggtcgcc catggcgagc gcctccgctt cgcttcccgt gacatgcaga aatgtgacgc
    19141 ccaaccggct gcgtatccgg cgcaattcac cgcgcattcg catgcgaaga ttggcgtcca
    19201 gcgcgccgag gggctcgtcg agcaggacca ggcgcggttc ggtgacgagc gttcgtgcca
    19261 gcgccacgcg ttgccgctgc ccgccggata tctggtggac cccgcgcgat tccagaccgg
    19321 cgagaccgac cagatcgatc atggcgcgaa cccggtcgcg cacctcgctt gccccgagag
    19381 gagcgccttc ggcgcggttc tcaagaccga aggcgatgtt tgcatatacc gacagatgcg
    19441 gaaacaacgc aaaattctgg aacacgaagc cgatgccgcg gctgtaggtg ggcgtgccga
    19501 ccatctggcg gccggcgaag acgatcgccc ccgcgtcggg ctgctcgaaa cccgcgatga
    19561 ttcgcaacag cgacgtcttg cccgaaccac tggcgccgag cagggacaca tagcagttgc
    19621 cgggaatgtc gaggttgatg ttgtcgagcg cgaccgtcgt ggcatagcgc ttggtcaggc
    19681 cggcaatatg cagaaccccg gtgaccacaa acacactcct cgaactaccg atacgcgggg
    19741 tggcgggaag ccgcagccgg aacgtcggac atgccttccg tcgttctcag cctagagaat
    19801 tgtcttttgt gttcaagcaa attttttgag cattgtggtg agaagatttg ctgcgacttg
    19861 gcgttttcct cgtcatgact ccaaccaaca tggagcatgt ccgataacgc ccgaatgtga
    19921 cggcaaattg gcaaattccg acgaaaccga agagctgaca gatccgatga tgcgggacgg
    19981 agcaaggcga ccaggctgct gatcagccga aaaattacgg caaaagcaga aattagcttg
    20041 ttaataatgg atatcgtgct caaagctcat taaattacgc gtgttctcac ctcttatgcc
    20101 agggcttaag gcctcgaaat gatcctctcg cggcggggaa acaaccgatg aaacgtcccg
    20161 cgaaccctcg cccggcttcg cggcgctacg agatggttga ggaggtgctg cgtcagaaca
    20221 ttctcggagg tctcattccg cgtggcctgg tgctgcttga aggaccgatc gccgcgctga
    20281 tgcagaccag tcgcgcgccg gtgcagacgg cgctgcaacg cctggaagag cagggcatgg
    20341 tccggcgctt caggggacgc ggcttcatgg tggcgaagcc cggcgaggtg ctggagccga
    20401 tcaggttgga tcttcagggc atcggcctgc gcatccccaa agccatcgac gacgccttgc
    20461 agagtcgcgc gtcctgggag cgcatttatg agatcgtcga gcacgatgtc gccgcctgtc
    20521 tcgtgttcgg gcgttatcgc ctgatcgaag ccgaactcgc cacgcatttc agggtgagcc
    20581 ggacggtcgt gcgcgacgtg ctggggcgac tggagcaacg cggccttgtc ataaaaaacc
    20641 aaagctcgca ttggatcgcc gagccgctca ccgcccagtc gattcgcgaa catttcgcgc
    20701 tgcgcaggat cctcgagccg caagccctga caagtgcggc gccgctgctc gaccgcacgc
    20761 gccttgccgc cgtcttcgac cgcttccgcc aggccgaagc ggccttcgat gccgatcact
    20821 tgaacgatcc actggcgcgc gatctcgaag ccttcgagac gctgttgatc gacacctgcg
    20881 tgttcgcaac gcccaaccgg cgcctgcgcg agcagatccg gcacaatctt ctcccggtcc
    20941 ttgccgccga gcgcgccctt cggcaactcg gtctgccaag cgaccgcgaa gcggtcacgg
    21001 agcatcgctt gatcgtcgaa ctgttgctcc aggatgcgat cccggcggcg gccgcgatgc
    21061 tggatgcgca tctggcggcc tccgagcgcc gcagcatcgc ccagcttaaa atcgtcgcgg
    21121 tgctgtccga gccgcaatcg gtcgcgcgct atctgacgcg actggattga agcggcggga
    21181 caggacatcc cgctcaaacc aggatgtcgc ggatgctttg catgacacct tggcgatcgc
    21241 gccgcgccgc caatgcttgt tgcatgtcga ccttgacgaa acgggtcggc gtgtttggct
    21301 gaagctgccc gatcaggtcc atgtcggcgg aaataaccgt tcccagcatg aagtagccgc
    21361 cgcccgacac cgcgtcgcgg tgcaagatga tcggctcggt gccgcccggc acctggatcg
    21421 aaccataggg atagcaggcg tcgacgatgt tggaagggtc cgagcccgcg ccgaaaggct
    21481 gttcgcgctc gacgaacttc agtggccggc caccccggaa gcgatagccc atccggtcgg
    21541 cctcgggcgc gaccttccag ggatcgtcga agaatccctg ctgggattcc tcgaccaccc
    21601 tatgccaata aagacccggc agcacccgca actccgcggg cattgccggc attcgccgca
    21661 atcgctccgg aatggtccgg ccctccccgg caaaagcaac cgttcccacc ggcagttcgt
    21721 cggactttgc gatcgcgcgg cctttgtagc cgccgagcgc gccgatcgga taggtcgaac
    21781 ggcttccgag tgcgagcggc acatcaaggc caccggaaat cgcaatgtag atacgagcgc
    21841 ccttctgcaa atagccgaag ctcaggatct gcccggcctt gatccgcagc gcggtccagg
    21901 atggcttctc ggcgccgtcg atcttgatcg gcatttcagc gccggtcacc gcaacgaccg
    21961 catccagcgt gaactccagt tcggggccga tgaatacggc ctctaggccg gcggcaccct
    22021 cgtcattgcc gaccagcagg ttggcggcac gcagcgccag gcgatccatc gcgcctccga
    22081 tcgggatgcc aagatggaaa tagccgggcc gtccaagatc ctgcaccgtg gtcgacaagc
    22141 cgggattgag caccttaacg gtcatcgagc gcgccctcca gcttggcgtt gcagccgtcg
    22201 atgtctttct ggaattccct gaggtcgaag gtgacactgc ggatggtcgg cgcgaaggtt
    22261 ccggccgcca cgtcctcaac cgcgcggtca tagtcatcgc ggccgatcgg cttgaacttg
    22321 acgatgtcgc cagggcgaaa gaacaccatg aagtctctga gataactgat cttctgctcg
    22381 gcatcgaaga tcggcatcgg ggtgatgccg aacatctgat aaccgccggc gccacgcacc
    22441 gagtagatgc aggaaaaaca gccgccatga ccgatcgtga gctttggcgt gtcggtgcgc
    22501 ggccgcagat atttcgggac ttgcagctgc ctcgggcggt cgaccatctg gtacaagaag
    22561 ggcagcccgg ccacgaatcc aaccatcgag acgaaccagg gcgagccgct gtgggccgcg
    22621 atgaacgcgt ctaccccgga aagcccgttg acttgcgcgg catattcgag atcggtcgcc
    22681 ttgggatcct gatggcgctc acgaaagcgc atcagggtct cgtgggtcca gggatcctga
    22741 tagaacaccg gaatctcgat gatgcgggtc ttgatcaccg gctcggtttt ttcggccgcg
    22801 ccttcgatgg ccgtaacctc gcgcaagaga tcgtcgggct tgatgacgtc gggatcgaac
    22861 ttgatctgga acgaggcgtt ggccgggcag atctcggtca ccccggcgat gcggctttcg
    22921 cgcaccgcgc tggtcatcga caggctcttg aagaaagcct caagcgacat gctttcatcg
    22981 cactcgacga aaagatgctc gtcgcctcca aatgagtagc ggttgctcat cagaccacct
    23041 ctttgacact ggcaggtgcg gcgaaccggg cctccagcca ttgctcgaga aagcgcgtgt
    23101 ggaagcggcc ggccacgaca tcgggatcga gggcgagcgc tgtgtggagc ggcaaggtgg
    23161 tcgggatgcc gacgatcttg aggctgcgca gcgccgcgcg aagtcttgca aggcaatcgg
    23221 ttctgttcgc gccatgcaca atcagtttgc cgagcagcga gtcgtagaac gggggcacct
    23281 catagccgtc gaacagcagc gtgtcgaagc gtatgccgtc gccggaaggt gtctcgagtt
    23341 tttcgacgac gccgggccat ggcatgaaat tccgatcagg atcctccgca ttgatgcggc
    23401 attcgatcgc gtgacccctg atcgcgatct cgtcctggcc aaccgaaagc ggcaggccgc
    23461 cggcgatccg cagcatctcc tggacgaggt cgaggccggt gaccatctcg gtaacgggat
    23521 gctcgacctg gatccgcgtg ttgacttcga tgaagtagaa ggccttcgtc gcctcgtcat
    23581 agagatattc gaccgttccg gcgccctgat agccgaccga gcgggcgagc gcgacggcgc
    23641 tcgcgcagag tttttcccgc acgccggcag gaagccccac cgccggcgct tcttcccaga
    23701 ccttttggcg gcggcgctgc agcgaacatt cgcgctcaaa acaatggacg aaatttgtgc
    23761 cgtcgccgag gatctgcacc tcgacatgcc gcgccttctc gatgactttc tcgagataga
    23821 gaccgccgtc gccgaaagcg gcggtggctt ccgccgcggc ctgcggaaac tggcgatcga
    23881 actcgtcgaa atcctgcgcg atgcggatgc cgcgtccgcc gccgcctgcc gcggccttga
    23941 tcatgacggg aaagccaatt ttagagacga tcgcccgggc ctcgataaga tccaacacgc
    24001 ggccgtcact gcctggaacc gtcggcacac cggccattgc cgcgacctgc cgtgccgcga
    24061 ccttgtcgcc gagaaggcgg atggcggcgc cggaggggcc gacgaagatc atgcccgctg
    24121 ccacgaccgc gtcggcgaag tcaccatttt cggacaggaa tccataaccg ggatgaacgg
    24181 catcgactcc cgccgcggcg gccgcggcaa gtatcgcatc gacgttcaga taggatttcc
    24241 tcgccgccgg cggtccaatc tcgatcgcct cgtcggcaag cctgacggca agcgacttcg
    24301 catccgcctg gctgtagacc tgcaccgtgc gcagtccaag cgccttggcc gccttgatga
    24361 tgcggacggc gatctctcct cggttggcga tgaggaccga gcggatattc acgcatcgac
    24421 ctccgccagc gtctgcccgg ccatgatcgc gtcggcgtcc tcgaccagaa atcggatcgc
    24481 cttgccggca acacccgcct tgacctcatg gaacgacttc atcacctcga ccagcccaat
    24541 gacgtcatcg gccgtcacgg gatcgccgtc gttccgaaac ggcggggcct ccggcgaagg
    24601 ccggcgatag aacatgccgg gcagcggcga cttgatttcg agtttgctca ttgcggtttc
    24661 ctgaaggctt cagcgcgccg agccagtacc tcggacggcg gcgcgatccg cacgccattg
    24721 gcgacgagcg ctgcgcgcat ggttctgccg atttcaagcg agccgggcgt atccgagtga
    24781 aagcagatcg aatcgaattc gatgtcgatg tcgtcgccct cgaccgtgcg cacccggccc
    24841 tcgcggcagg cccgcacgac cttgtcggca atggtgcgcg gatcgagctt gccggcgccg
    24901 cgcgtgaaga cgattgaccc ggaacggtcg tagtcgcgat cggcataaaa ctcccggatc
    24961 acggggtggc cggcctcgcg ggcgatgcga taggtctcgg agacgctcat gcagaagata
    25021 aaagcgtccg gcgcaagcgc gcgcatgaac tcgacaaagg ccttcgacag cgcctcgttg
    25081 accgcaagct ccatatagag cgcgccgtgc ggcttgacgt gctggagcga ggcgccgtgc
    25141 cggcgcgcga actcgcgcag cgccccgacc tgatagatca tgtcgttgat aatctcgtcg
    25201 ccgcttccat tgaccttgcg ccggccaaaa ccttgcaggt cgtgatagcc gggatggacg
    25261 ccgacaccga cgccgtgctg tgcggctagc cgaacggtcg tatccatcag gttcggatcg
    25321 ccggcgtgga agccggcggc gatgttggcc gagctgatca gcttcataag ctcggaatcg
    25381 tcggcgtcgc cgaggcgcca ccggccaaag gcttcgccca tatcgcaatt aatgtcgacg
    25441 atcgtgaccg ccattccaac cctcgttcgc gcgccaaacc gctttcgcgc gccgcgtctt
    25501 cgccacgtcg ctgcatctgc ccgccatgag gctagcgtcc tgtcccgaca ttggaaaatt
    25561 caaatatttg aacacagtct tcgtattttc agataccacc atttcacttg aattgtaaaa
    25621 agcttttggg gactgatgaa aatgcccatc gcgttgctca gttgtgaagc agtccctgct
    25681 ccgcaaaggg gcagcgatct tatccaccgt gcttttctgg tcccggcatt tgaaaaacag
    25741 aaggaaacgt gatgtcgatc agcctccggc aaatccgcta cttcgtatcg accgccgagc
    25801 acggtcaggt ttcgcaggcc gcgttggacc tcgcgatttc acaatcggcg atcaccacgg
    25861 cgatacagca gatcgagcgc acgatcggcg cggacttgtt cacccggtcg cagcaaggca
    25921 tggagctgac gccggcgggg cagcaattcc tctttcacgc ctatgacatt atcaacaagg
    25981 tggcggaggc gaccagtccg agcatccccg caaacgatct cgaggggcgc ctttccgtcg
    26041 ctgcgactta caccgtgatc ggctattttc ttcccaatca cctcgagaag atgaaacgcg
    26101 gcctgcccaa gctcgatatt cagctgtttg agctgaaccg cgaggcgatt gaggaaggcc
    26161 tgctcgcgaa ccgctatgac atcgcagttc tcctgacgtc caacgtcctc aacccggact
    26221 tgtcgaccga aacgctgctc agttcgagac ggcgtctctg ggttccttca aaacatccac
    26281 tgctgtccga aagctcggtc ggcctgaagg agatatccga acagccctat gtgatgctga
    26341 cggtcgacga agccgcgcat acctcgctca aatattggag caacacgccc tatcagccgt
    26401 cggtgacgct acgcacctcc tcggtagagg ccgtaagatc gatggttgcc aacgggctgg
    26461 gcgtcacgat tctctcggac atggtgctcc ggccctggtc gctcgaggga aagcgcatcg
    26521 aaacaatcgt gccacgtaat ccgatccctc ccatggacgt cggcctggcg tggcggcgca
    26581 acgcccactt caccccggtg atggaggcgt ttcggtccta tttccgacag acatttttta
    26641 ttccgacagt gcgataaatt ttattccgac agtgcgataa agcgggcatc gtgcccaagg
    26701 ccgcgggcaa gatcttccgg ggaaggatct gcccaggtac cgggcgggca cctgtcacct
    26761 tactattcgg aagatgaaga cgaccccata acgacttagc ccttcagggc ttttttgtcc
    26821 gctagcgccg atgacccagc ctgattccaa gcttgagcgc cttctgtcgc agcgatcctt
    26881 cggtacgctt tgtgagcttt gcaatcttgg cgaccggcgt tttcgccttc gagtgtgccc
    26941 gcaactcttt cacatccgct cgtgtatatt cgcggcgcaa gacagttttc ttcgcttttt
    27001 tagccactcg gtgctcctga ttttcggaat cgctcttcta gcacagattt cgccgccgcc
    27061 cactccaact tgcggcgcat tccggatagc agcgtcggct cgcttgcaag aatcgaaaat
    27121 ccgggaagtc gccttgccac cgtcaatttt cgtggaggcg gctgccctat tggccacaga
    27181 cactccacta actcaaactt aaaatgtttg tgccaagatc tgtaccaaga acagcacgcc
    27241 tcggcttcgc gcaagcagca tctatcaaat cctccaaggg acagccatgc ctcagatctg
    27301 gatgacctac gaagaactgg cgagcatgct ggattgcccc gtggcggaag ctcgtgagcg
    27361 ggcgcacctc gaacgcctcg atcggaagat cagccacgat ggaaaaaccc gcgcaaagct
    27421 cagcgtggca atgaccggta ttttcatcga gcggctcaag acgatcgatt atgacataga
    27481 ccgtgtggtt aacaatctta gacttgtcca tggtctcctc agtgagtgcg tcgaccaatc
    27541 cgcacacttg acgtcacaac ggcgccgctc gcatcaatcg ggttaaattt cgcaactgag
    27601 cagccttcag aagcgaaggc tgtcatcgaa cgcagcctcg ccgccggcga caggctattg
    27661 gagcgaggct cgttcgaagc cggcgatctg acgatctatc gcggcatcaa cggcgatttc
    27721 catgacacgg tgctggccgc ctcgcgcaac cgcatgctcg gcgagatgat ccggacctgc
    27781 catcgcgtcc cggtatcgtc gagccgcaac atcgtcgcct tcgagcatcg tgacgtgcgc
    27841 cgccgccacg acgatcatca ccgcattcat gaagcgatca tcgcaggcga accctggcgc
    27901 gccgaatggc tgatgcgcga acacgtctcg agcgtcaagg gatcgttgat cagggcgctg
    27961 gccgcgcgca acgccgatca agcggatcaa acggacagac cgtcgacttg gttttggcga
    28021 cggtactgac ctcggctccg tatcaccgtt gcccgatatc tacttgcggc tcttgcgaga
    28081 tccggtccgc ccaggctttc cctttagcct cgctgtggcg tcgcggcggc gcggcaccaa
    28141 cgtcgtctct ctgtcgacgt tggcttgcac gcgaaggccg tcgacgaaga catcgagcag
    28201 ccgcagcacg ctcttctgcc agccgggctg gtcgtgcata tagcacatgc cgacgagcgc
    28261 ccgcagcagg tcttcggggc tgatatcgga gcgtatctcg ccagccgcga ccgcgcggtc
    28321 cagcaaggcg ccaaccgcct ttgtcaatcg atcgaacgaa taggcgtaga gttccgacga
    28381 gccatgcacg gcaagcgcca gcgccgccga catgcccttc ttggtggcaa caaattcgac
    28441 attggatcgc agccaacggc gcaaggcgtc gaccggctcg gcctcgctct tcaactgttc
    28501 cgccaggtcg ccgagctgct gcacctcgcg tcgatagact gcctcgaaca aggcctcgcg
    28561 ggtcgggaag tggcggtaaa gcgtcccgat gccgacgccg gcccgttttg ccacagcctc
    28621 gaggctcgcg tccggaccac cggcgctgaa cacagccttc gcggcctcca ggacgcgctc
    28681 gcggttgcgc accgcatcgg cgcggggcct gcgaacagtg gctgcggact gatctgccat
    28741 ccgattattt tggatcacga tccggttaga ttgaattgga ccatcatctc atctctttgt
    28801 ttgaacatga tcttttgtca aaatcaattt ccattttccg gatcatgctc tggttgtcct
    28861 cgtcttgcaa aacttcttct cttgcaaaac ggaggatgcc tccgtatatg ccgatcagca
    28921 ccgaacttgc acgtgcccgt cgatcaaatc ccggcggccg cgatgcagtt tcaccatagc
    28981 attgaccgga gcactgcatg aacctctcca tcagcctcac cgttaacggg gtgcgacggg
    29041 acgtcgagct gggagacccg cgcgtcacgc tgctcgatct cctgcgagag cgtctccatc
    29101 tcacaggaac caagaaggga tgcgaccgcg gccaatgcgg cgcctgtacg atcctggtcg
    29161 acggcggacg gatcaattcg tgcctggctt tggcgctcag ccacgacggc gccgacatcc
    29221 tcacgatcga aggggtttct cgcggggacc aactgcatcc ggtccaggcc gccttcatcg
    29281 cgcatgacgg cttccagtgc gggttctgta cgccgggcca gatcatgagc gcgatcggcc
    29341 tgatcgggga agggcaagcc ggcgatgatc ctgagcgcgt gcgcgaaggt atgagcggaa
    29401 acctctgccg atgcggggct tattccggga tcaccgacgc ggtgctggac gtgcaacaaa
    29461 gcctcatcga ggcggaccag aggcgctcgg catgaagacc ttcgactatt tcaggcccgc
    29521 aacgatctcc gacgccgtcg cggctgcggc cgaaccaggc gctgcctatc tcgcggccgg
    29581 caccaacctg ctcgacctga tgaagggtgg ggtaaccggc ccgagccgac tcgtcgatgt
    29641 cacccgtctg ccaggtctcg accgcatcga acgcctgccc gacggcggca tccgtatcgg
    29701 cgcgctcgtt cgcaatgccg atctcgcgca cgacccggat ttcgcaaaat cctatcccgc
    29761 gattgccgag gcgctgctgt ccggcgcatc ggcgcagctt cgcaatgccg cgaccgtcgg
    29821 cggcaatctg ctgcagcgga cccgatgcgg atatttctac gacgtcgcca gcgcctgcaa
    29881 caagcgtgag ccgggtgcgg gttgcgaagc ccgcggcggc gagaaccgat tgcacgccgt
    29941 gctgggttgg agcgaggcct gtatcgccac ccatccgtcg gatttctgcg tgccgctggt
    30001 cgccttcgac gccgtcgtgg agatcaaagg cagcaatgga aagcgcgagg tcgcggtcga
    30061 ggccctgcat tgtctgccgg gcgatacgcc cgagcgcgaa actgtactcg agccgggtga
    30121 cttgatcgtc gcgctacggc tgccgggcca ggcacgcgcg ttctcggcgc atgcgcgcta
    30181 tctcaaggtc cgggaacgca cttcctacgc cttcgccgtc gtgtcagccg ccgcaggctt
    30241 acggatcgaa caaggcacga tccgggatgc ccggctcgcg ctcggcggcg ttgccgccaa
    30301 accgtggcga gctcgcgcgg ccgaggaaat gctggcgggc tcccgccccg gaccagatgc
    30361 cttccgccgc gcggcgaagg ccgcacttgc cgacgccaaa ccttccggtg acaaccagtt
    30421 caagatcgag ctcgcgcagc ggattctcgt gcgcgccctg acgctcgccg cggcaggaac
    30481 cccgcagcgc attccggccc tgccggcctc ccctttctca tccgtatccg gagcattgca
    30541 tgacgcctga gatcagcctc acccaagcgc cagcccatgc ccggcacggc tcgaatatcg
    30601 ggcagccgct gacccgccgc gacggcgtcc tcaaagtcaa aggagaagcc cgctacgcgg
    30661 cggacaacca tccgcccggc atgcttcatg ccgttctggc ggtctctggc atcgcgcgcg
    30721 gtcgcgtgag cttcctcgac gtgcaagccg ccaggagcca cccgggcgtc gtcgacgtca
    30781 tgacccccgc caacaggccg ccgctcgcgg aggaccccga cgcgaagacg aacccgttca
    30841 tgttccggct cgatctcctg caaaacgacc aagtgcgtta tgcgaaccag tcgatcgcgg
    30901 tggtgatcgc cgagacgctg gaggccgcga cggaaggcgc ggcgctgttg tcgccgcgat
    30961 acgaagcgca accggcccgc gtcggtctcg atgccggcga aagcttcgtg ccatcagccg
    31021 tcggcatcgg caatccgtct gaagtacgac ttggcgatgt ggaggcgggt ctggccgcgg
    31081 catcgaagcg catcgatgcg acctacgaga cgccggccca gtaccacaat gccatggagc
    31141 cgcacgcgat cgtggcggcg tgggacggcg atacgctgtc gatcgatacg ccgagccagg
    31201 gcctcgccat ggcgcaaggg cggatcgcgg gactgttcgg catttcaccg gagaagatcc
    31261 atattcgcag ccccttcctg ggcggtggct tcggctcaaa gggcttcatc tcgggaccgc
    31321 aggtcctcgg tatcctggcg gcccggctgg tcaggcggcc ggtcaagctc gtgctgcgcc
    31381 gtgaacacat gtacggtcca gtgggccatc gtgcgccgac ccgccagcgg cttcgcatcg
    31441 gcacggaccg cgacggactg ctgaccgcga tcgatcatca cgccaagacg gtatcgagca
    31501 cgttcgacga tttcttcgag ccggccgccg acgcctcgca tacgctttac gcaagccctg
    31561 ccatcgccac ctcgcacgag gcggtccgga ttgacaccgg cacgccgttg ttcatgcgag
    31621 ctcccggtga agcgaccgga tcgattgcgc tggagagtgc gatcgacgag gcggcatggg
    31681 cctgcgggat cgacccgctc accttccgca tcaagaacta tgccgaagtc gaaccgattt
    31741 ccggcaaacc gttctcctca aaggccttgc gcgagtgtta cgcgcaaggc gccgaacgct
    31801 tcggctggtc aagacgccca ctcgcaccgc gacagttgcg tgacgaggcg ggcctcctcg
    31861 tcggctgggg aatcggcacc gcgacattcc cggcgctgat gttccaggcc gaagcgcgtg
    31921 ccgtggtgcg cagcgacggg tccggcctga tggaaacggg agcccacgac atgggtcagg
    31981 gcgcctggac cgcgctcgcg caaattgccg ccgatgggct cggcctcgaa ctcgagcaaa
    32041 tggagttcag ggctggtacg tcagacctgc cggacgccgg catcgccggc ggctcggccc
    32101 acaccgccac ggcgggtatg gcgatccaca atgcgggcgc cgcggtgatc ggaaagctcg
    32161 ctgacctggc caccggtgac gagcgctcac ccttgtttgg tgcgggcaat gccggggtgc
    32221 tcgcgcgtga cggccgcctg ttccgtcgcg acgacgaaac gcgaagcgag agctatgccg
    32281 atatcctcgg ccgcgctggc ctccgcgaga tcgaggcccg cggcaagggc gccgccgacc
    32341 ctgcggcgca atcgacctac gccatgcacg cgcacggcgc ggtgttcgcc gaggtcaagg
    32401 tcgaccctga tttgggtcaa atccgcgtca cccgcatggt cggcgctttc gctgcggggc
    32461 gggtcatcaa tcctcgtctc gtccgcagtc agatctttgg cggcatgatc tggggcgtgt
    32521 catttgcact gcacgagcat gccgtcatgg accggcgctc cggccggata ctaaacgcca
    32581 atcttgcgga ttatcatgtg ccggtgaacg ccgacgtgcc ggggctggaa gcgctgatga
    32641 tcgatgagca cgatccccac gtcaacgccc tcggcatcaa gggggtcggt gaaatcggcg
    32701 tcaccggctc ggccggcgct gtcgcgaacg cggtctggca cgccaccgga atccgggtgc
    32761 gtcgtttccc gatccgcatc gacgatctgg tgacgcctgc ttcacattaa ttccatggag
    32821 acggcaggcg ctacaaatca atgccggtcc atggtgggct gggacagata ggccagcagc
    32881 ttgatctcgc ggcgcccgcg cgtcacggta tcgagcacca gccccgacga taccgatagc
    32941 accgccagga tcatcaggcc catcgacaga accgcggtcg gcagccgcgg aacgatgcct
    33001 tgctcgagat aggtgacaat gacggggatc gcgagcccga tcgaaaccag catcaggaaa
    33061 actccgatcg ccgtgaag
//
LOCUS       DASWGM010000029         6296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1664858,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000029 DASWGM010000000
VERSION     DASWGM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664858"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..1706)
                     /locus_tag="VGA15_01085"
     CDS             complement(447..1706)
                     /locus_tag="VGA15_01085"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9586325.1"
                     /translation="MTARPQLPDRTPRAEGVPTALNGLLVVDFTRVVAGPACTQTLAD
                     FGAEVIKIENPDGGDDTRSYEHADLAGESAAFVSLNRNKRGIVLDLTKSEACDVAREL
                     IARADVVVENFSAGVMKKYDLDYASVAPSNPRLVYCSISAYGRQGPFAFRPGFDPITQ
                     AESGFMSLNGFPDGPPVRTGPPAIDMVTGMSACNAILLALLARDRLGRGQLVEVALFD
                     IAVAMTQFYGMAYLMSGVNPSRQGNSPNGSPSVGVYDGSDAPFYIACANDRLFRRLVI
                     EVLGRPDLASGEFADRRSRSANKAKLRAILTGIFAQDHRESWVAKMKAANVPVGYLRT
                     VEEAFNSPEVRERHRLSQIPHPAAGSVPNIESPLHLGLTPVVDPIAAPLLGEHTKAVL
                     RRTLGYDDGRIAALAEAGVFGAAPGAP"
     gene            complement(2065..2931)
                     /locus_tag="VGA15_01090"
     CDS             complement(2065..2931)
                     /locus_tag="VGA15_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9586326.1"
                     /translation="MLTKQAPTHEYKYVPTDFGNIAYREAGSGPVAIFVHGVLLNGHF
                     WDRLIERLADVRRCIAIDILAHGATGAGADQDLSFDAQASMLAAFCQAMTFDKVDVVA
                     NDSGGGIVQIFAARHPGRVRSLALTDCDTHDNWPPKEAEPLHRLAMEGGLGAVGRRML
                     EDVAFARASFGAGFERPDQLSPEDFRTWLEPLFKSPASTRNLERFIASFDNRQTVAVE
                     PLLRKLEAPTLIVWGTADVFFPVKWAYWLRDTIPGARQVVELEGAKLFLPWERPVELA
                     AALRNFWGIELN"
     gene            complement(3011..3385)
                     /locus_tag="VGA15_01095"
     CDS             complement(3011..3385)
                     /locus_tag="VGA15_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586327.1"
                     /translation="MINIVFFLLTTWSSATAPESFAAKLGLGIVNAGGINEVRAQYSG
                     FFLAAALVCTASLFGLLSRQTSFVVVGAVFGGLFAGRLVSLALDGGVAGYGPTILALY
                     AIDAIGLALAVAAYVLDNQAKG"
     gene            3533..4120
                     /locus_tag="VGA15_01100"
     CDS             3533..4120
                     /locus_tag="VGA15_01100"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9586328.1"
                     /translation="MVKSRKEQNLEATRAALMAVARKHFARDGYSDAEIAKIAADARV
                     TTGAVYHHFASKKGLFVAVAEDLEAEILGTAASVTSDDPWQRLRLGFEKLIDLCASPA
                     VQRIIFVEAPQVVGPDAWRKIELRYAFGTLRTVLTALRDANIIKPYPVDLIARTLLAL
                     LHETSAELARSKHDPKIRAQISDLVAGVLDVFLVR"
     gene            complement(4214..5266)
                     /locus_tag="VGA15_01105"
     CDS             complement(4214..5266)
                     /locus_tag="VGA15_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586329.1"
                     /translation="MRYAALVAILFLLIPPAGASTTPEIDPRASLSVVQQWIYNYRAK
                     PDYAHVPAAVRVLFHAQSFKEPENSGIYLGFIAGAIGSNPARAEQLVTSFFPVAPEDE
                     WVIVRAIAYSGLPDWKNLLRQVAPRMPGRKVMIDSYLAGTLPTLANIPLEESKPGMLD
                     KLRGVFTKNPFAKDDKRLKMTQTFAGNQDLLDTLWGYYFATGSHLPILRIMQMLPWSK
                     SRDTVDKLTVGSMARYTLASYAVRDAGLREFLRSEQASQPEAIKVPLGEVIEAADTVQ
                     LGAVRKDALAAVEELKAKGSDSRRNLDFWGQVGVGAVALGCVSAAALGQVAVGIPCVI
                     GGSASQGLLSFWEKQQ"
     gene            5518..>6296
                     /locus_tag="VGA15_01110"
     CDS             5518..>6296
                     /locus_tag="VGA15_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX9586330.1"
                     /translation="MAPNGRCYRSISAGVLLVTALGLFSAPTLAAEAAAQARETIVVQ
                     GNRRVDAETIRSYFHATPDGRFDAAARDAALKALLATGLFDNVAIDRAGERLVVHLTE
                     APVLDRVAFEGNKKIKDTELTAVIESKPRGTLQRAVVQADVGRIVEAYRHVGRDDVGV
                     VPQIIDRGSDQVDLVYVVTEGGKTTVRQINFVGNHIFGKRQLGAVIKTSATNALSFLT
                     GGNVYDPDRVAEDREQLRLYYRSKGYADASVPSANAEYDPAT"
BASE COUNT         1297 a         1925 c         1919 g         1155 t
ORIGIN      
        1 ggtcgatact gcggctaagg aggctgaacg tctcggcctg acgacagcta ccttgatact
       61 tcgaatggca cgcctggaaa ttgaccgggc cgaacccgac gaggttgaaa gtatgccaag
      121 gaacaactgg cgcggcaagc ctaactgata ggccgaaaac gccgcgaccg ccctaatcgt
      181 gccagattcg gttcggtatg gcgggttcgg ttcctgtgtc gccgccgatt ggacataatg
      241 tcgatttgcc aatcgatatc cgccccggcg atttagtcgg tgcagccatt atcgctttgg
      301 cggaaatagt ccgccgcaat agcaaggccg cgaaccacgg gcgtcacaac agcttttcca
      361 taccggactg gagcgcggtt gccatctggt cagccagttg cctgcgccgc tccgttcacc
      421 ccgaatggtg agcgccccaa gcgacgtcac ggtgcaccgg gcgcggcccc aaaaaccccc
      481 gcttcggcaa gcgctgcgat gcgaccatcg tcatagccga gagtcctgcg cagcactgcc
      541 ttggtatgtt cgcccagcaa gggcgccgct atcgggtcaa cgaccggcgt caggccgaga
      601 tggagcggcg attcgatatt ggggacggag cccgcagccg ggtgcggaat ctggctgagg
      661 cgatggcgtt cacgcacttc cggcgagttg aaagcctcct cgacggtacg aagatagccg
      721 accggtacat tagccgcctt catcttcgcc acccagctct cgcgatggtc ttgcgcaaaa
      781 atccctgtga ggatcgcacg cagtttcgcc ttgttggccg atcgcgagcg ccggtctgcg
      841 aattcaccgc tggcgagatc cggccggccg agcacctcga tgacgaggcg gcgaaacagc
      901 cggtcattgg cgcaggcgat gtaaaacggc gcgtcggacc cgtcgtagac cccgacagag
      961 ggagacccgt tcggcgaatt accctgacgg ctcggattca caccggacat caaataggcc
     1021 atgccgtaaa attgagtcat cgccaccgcg atgtcgaaca gcgccacctc aacaagctga
     1081 ccgcggccga gcctatcgcg ggcgagcagc gcgagcagga tggcgttgca ggctgacatg
     1141 cccgtcacca tgtcgatcgc gggtggcccg gtccgcaccg gtggcccatc aggaaagccg
     1201 ttgagcgaca tgaatccgct ttcggcctgc gtaatcggat cgaaaccggg tcggaatgca
     1261 aacggcccct gacggccata ggccgagatc gagcagtaga ccaatcgggg gttgctcggc
     1321 gcaaccgaag catagtcgag gtcgtacttc ttcatcacgc cggcggagaa gttttccacc
     1381 accacatccg ctcgcgcgat caattcccgc gccacgtcac acgcctcgga cttggtgaga
     1441 tcgaggacga tgccgcgctt gttgcgattc aaactgacaa aagcagcact ttcacccgca
     1501 agatcggcgt gttcatagct gcgcgtgtcg tcgccgccat ccgggttctc gatcttgatg
     1561 acttcggcgc caaaatcggc cagggtttgc gtgcaggcgg gaccggcgac gacccgggtg
     1621 aaatccacca ccagcagacc gttgagggcg gtcggcacgc cctcggcgcg tggcgtgcga
     1681 tccggcaatt gcggtctggc agtcatgatc ggtgtttccc ctttttatat tgattggcag
     1741 tcttcaggtt gagtactagg tcatactttg aagcctgctc aactggcaaa tcggaaggcc
     1801 agccctgcgc gagcccggat aaacgagatt cggcccgttt ctctggcgat gccaagccgg
     1861 agaacacggc cggaaatgca gcaaaaaaat aaggggagcg tgagatcgtc tttgttaagc
     1921 cagcggttaa gccagcggga cgaatgcacg cccgatggcg ggccccgcaa agcaatccgc
     1981 gagccagagc tggcccggac tggcaagcgc caaacccgca atgacaggat gggcgcgcaa
     2041 cgtcgtcgca aaaagcaaaa tcgttcagtt cagctcgatc ccccagaaat tgcgcagcgc
     2101 ggcggcaagt tcaactggcc gctcccaggg aaggaacaat tttgcgcctt cgagttcaac
     2161 gacttggcgc gctcccggga tggtgtcgcg cagccaataa gcccatttca cgggaaagaa
     2221 gacatcggcg gtgccccaca caatcagcgt cggagcctct aatttgcgaa gcaagggctc
     2281 gacagcaacg gtctgtctgt tgtcgaaaga cgcaataaac cgttcgaggt tgcgcgtcga
     2341 tgcgggagat ttaaacaggg gttcaagcca ggttctgaag tcctcgggcg atagctgatc
     2401 cggacgctcg aagccagcgc caaagcttgc tctggcgaaa gccacgtctt cgagcatgcg
     2461 ccggccgacg gcgccaaggc cgccctccat tgccagcctg tgcagcggct cggcctcctt
     2521 tggcggccag ttatcgtgcg tatcgcaatc ggtgagtgca agacttctga cgcgcccggg
     2581 gtgccgagcg gcgaaaatct ggacgatccc gccgccgctg tcattggcga cgacgtccac
     2641 cttgtcgaat gtcatggcct ggcagaacgc tgcgagcatg ctcgcctggg cgtcgaatga
     2701 aagatcctgg tcggcaccgg cgcctgtggc accatgcgcg agaatatcaa tcgcgatgca
     2761 gcgcctgaca tccgcaagcc gctcgatgag cctgtcccag aaatgaccgt tcaggagaac
     2821 gccatgaaca aaaatcgcca ccggtcccga tccggcttcc cggtatgcga tgtttccgaa
     2881 atcggtcgga acatatttgt actcgtgagt gggcgcctgt ttggtcagca tttcggtgtg
     2941 tccctagttg tggtctgtcg gatgcggaat cttgcgcggg cagaggcgaa atctcgtgcc
     3001 gccagtgccc tcaccccttg gcctgattgt ccaaaacata agctgccacc gccagggcga
     3061 gaccgatggc gtcgatggca taaagcgcga gaatggtggg cccgtagccc gccacacctc
     3121 catcgagtgc aaggctgacc agacggccgg cgaacaagcc gccgaacacg gcgcccacaa
     3181 caacaaacga tgtctgtcgc gacagcagac cgaagagaga ggcggtgcag acaagagcgg
     3241 ccgccaggaa gaaccctgaa tactgcgccc tgacttcgtt gatgccgccg gcgttgacga
     3301 tcccgagccc tagctttgcg gcaaagcttt ccggcgcggt ggcgctgctc catgtcgtca
     3361 acaggaagaa cacgatgttg atcaccagaa cggcggtccc aaacactggc ggcatgtcga
     3421 aacctttcag atgcgcagct tataaattag aatgctcgca cggtacgtat cgatttgcat
     3481 aattcaatta tgttacattt ttacattatg tcaagtggct ggaaacgagg atatggtgaa
     3541 atcgcgcaag gaacagaacc tcgaagcaac ccgcgcggcg ctgatggcgg tggcccggaa
     3601 gcatttcgcc cgcgatggct attccgatgc ggagatcgcg aagatcgccg ccgacgcacg
     3661 ggtcacaacc ggcgcggtct atcatcactt cgccagcaag aagggactgt tcgtcgcggt
     3721 ggcggaggat ctggaggccg agatacttgg aacggccgct tcggttacat ccgatgatcc
     3781 ctggcaacgc ctgaggctgg gctttgaaaa attgatcgat ctctgcgcgt caccggccgt
     3841 acaacgcatt atcttcgtgg aggcgccgca ggtggtggga cccgacgcct ggcgcaagat
     3901 cgaacttcgc tacgcgttcg gcacgctacg caccgtgctt acggcactgc gcgacgcaaa
     3961 catcatcaag ccctaccccg tcgacctgat cgcacgtacg cttctggcgc tgttgcacga
     4021 aacttcggcc gagttggcgc gctccaagca cgacccgaaa attcgagcac agatcagcga
     4081 tctggtggca ggcgtgctgg atgtttttct cgtccgctga acgggccttt cggtgcgaga
     4141 ctatttcagc atcatccaca cctgaccgag ccgtccgatc cggccgggca cggacgcaaa
     4201 tacaagcctt ggatcactgc tgcttttccc agaacgacag caggccctgc gacgccgaac
     4261 caccgatcac acaggggatg ccgaccgcga cctggccaag cgccgccgcc gagacgcagc
     4321 cgagcgcgac cgcaccgacc ccgacctggc cccagaagtc gaggttgcgc ctgctatcgg
     4381 agcccttggc cttgagctct tccacggcgg cgagagcatc cttgcgcacc gcgccgagct
     4441 gcaccgtgtc ggcggcctcg atcacctcgc cgagcggcac cttgatcgcc tccggctggc
     4501 ttgcctgttc gctgcggaga aactcgcgca atccggcatc gcgcaccgca tagctcgcca
     4561 gcgtgtagcg cgccatactg ccgaccgtca gcttgtcgac ggtgtcacgg ctcttgctcc
     4621 agggcagcat ctgcatgatg cgcagaatcg gaaggtgcga gccagtcgcg aaataatatc
     4681 cccacaacgt atccagcagg tcctgattgc cagcgaaggt ctgcgtcatc ttcagcctct
     4741 tatcatcctt ggcaaacgga tttttggtga agacgccgcg taacttgtcg agcatgccgg
     4801 gttttgattc ttccagcgga atgttggcga gcgtcggcag agtgcctgcg aggtagctgt
     4861 cgatcatcac ttttcgtccg ggcattcgcg gcgcgacctg acgcagcaaa ttcttccagt
     4921 cgggcagccc cgaataggcg atggcgcgga cgatcaccca ttcgtcctcc ggtgctaccg
     4981 gaaagaagct ggtgacgagc tgttcggctc tggcggggtt cgatccgatc gcgccggcga
     5041 taaagccgag atagatgccg gaattctccg gctccttgaa actttgcgcg tggaacagca
     5101 cgcgcacggc cgcggggaca tgcgcataat cgggcttggc ccggtaattg tagatccact
     5161 gctggacgac gctgagcgaa gctcgtggat cgatctctgg cgtggtcgac gcacccgcgg
     5221 gtgggatcaa caggaatagg atggcgacga gtgctgcgta gcgcatgtga tgctcctggc
     5281 aggcgttccc gcatgcgacg gtcgcagcgc gaacgtttcg gccgcacggc taatacggat
     5341 ttgaagcggc tctgcgacaa ttctgggaac gccctgttcg gcgctgcgtc gaaattatgg
     5401 caagccgtta gcattcaagc ccgtaactga ccttatccca gtcattcttt tgcccggcgc
     5461 acgcaattcg attgcgctcc gccaaccgat agacaggtgg acgtgatgag gcgatgcatg
     5521 gcgccgaacg ggcgatgcta ccggtcgata tcagcgggcg tgctgctggt tacggccctt
     5581 gggctttttt ctgcgccaac gcttgccgcc gaggccgcag cgcaggcgcg cgagaccatc
     5641 gtcgtgcagg gcaatcgacg cgtcgacgcg gagacaatcc gttcgtattt tcatgcgaca
     5701 ccggatgggc ggttcgacgc cgcagcgcgc gacgcggcgc tcaaggcgtt gctggcaacc
     5761 ggcctgttcg acaatgtcgc gatcgaccgc gctggcgaac gtctggtcgt gcatctgact
     5821 gaagccccgg tgctcgaccg cgtcgcgttc gaaggcaaca agaaaataaa ggacaccgag
     5881 ctcacagccg tcatcgaatc caagccgcgc ggcacgctgc aacgtgccgt ggtgcaggcc
     5941 gacgtcggcc gcatcgtgga agcctaccgg catgtcgggc gcgacgatgt cggcgtggtg
     6001 ccgcagatca tcgaccgtgg cagcgaccag gtcgatctgg tttacgtggt gaccgagggt
     6061 ggcaagacga cggtgcggca gatcaacttc gtcggcaatc acattttcgg caagcgacag
     6121 cttggcgccg tgatcaagac ctccgccacc aatgcgctga gctttctgac cggcggcaac
     6181 gtctacgacc ccgaccgcgt cgcggaggac cgcgagcagc tgcgactata ctatcgcagc
     6241 aagggctatg ctgacgccag cgtcccgtcg gcgaacgccg aatacgatcc cgcaac
//
LOCUS       DASWGM010000030         5621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1387438,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000030 DASWGM010000000
VERSION     DASWGM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387438"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VGA15_01115"
     CDS             <1..491
                     /locus_tag="VGA15_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX9586331.1"
                     /translation="AQIYTTTDGRALDTIAISREYDRDEDEGRRATRIGEMIEDVLEG
                     KLQLPEVVARRSNRGKARPFVVEPEVIINNQWSDRYTVIEVSGLDRPGLLYQLTTAIS
                     KLNLNIASAHVATFGERARDVFYVTDLLGAQINAPTRQAAIKSALIHLLANDEDVAQP
                     AA"
     gene            529..1200
                     /locus_tag="VGA15_01120"
     CDS             529..1200
                     /locus_tag="VGA15_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX9586332.1"
                     /translation="MKLPRQARAVIFDMDGLMFNTETLYRDAVVAAAAEAGHDLPLPF
                     YLSTIGMSIEATRMAFSERYGKQFDFDGFWAAASTLYSGIVELQLCLKAGVVELLDFL
                     DGTSLSRAIATSSRHEDVQRNLGAQGLRDRFQTVIARGDYARGKPHPEPFLKAAERLG
                     VEPELCVALEDSYNGVRAASGAGMMTIMVPDLLPPTREMEELCVYIAQDLHEVHSLIR
                     SQPVP"
     gene            complement(1217..1621)
                     /locus_tag="VGA15_01125"
     CDS             complement(1217..1621)
                     /locus_tag="VGA15_01125"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9586333.1"
                     /translation="MTDAEVQDFVTRFAAAWAARDGQAFVELWHPEGLLHTPLVGRTV
                     AGSELNRLMEVQTAAAPDFVWQLLDWTARGDVVIIEWQTTRIVNGARFDWRGVDKFRI
                     KAGKIIEERVYCDTAPLRALRTGEALEPITRF"
     gene            complement(1908..2453)
                     /locus_tag="VGA15_01130"
     CDS             complement(1908..2453)
                     /locus_tag="VGA15_01130"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9586334.1"
                     /translation="MNAKPPETFGLDRLQTPIGIALLVTDAEGALCALDWEDYELRLR
                     QLLRLQYGATVLQDAQAPDDIRTALTGYFKGDLDRLSGIKWRVAGTPFQRKVWTALRT
                     IPAGTTLSYGALAAQLGMPKAVRAVGHANGSNPISVVVPCHRVIGADGSLTGYGGGLE
                     RKRWLLQHEGAALKAASRRAA"
     gene            complement(2434..4020)
                     /locus_tag="VGA15_01135"
     CDS             complement(2434..4020)
                     /locus_tag="VGA15_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX9586335.1"
                     /translation="MARVSGNPATMNDVRKPRDKLAAMDDASMMDMDRTACYRAIATR
                     DARFDGRLFVGVKTTGIYCRPICPARTPKFENVLFYPSAAAAQEAGFRPCLRCRPETS
                     PDLAFWRGTSNTVSRALALIEAGGLDEADVEGLANRLGVGARQLRRLFRQHVGASPIA
                     VAQTRRVLLAKQLIHETSLPMAEVALASGFNSVRRFNETFLQMFGRSPATLRHFRDRT
                     KREAGALSVRLAYRPPYDWEAMLSFLSARAIPGIEIVADNIYRRTISIGESCGVISVA
                     PADKHRVSVSVRFPDMAALPAIIARVRRVFDLATDPDMIGEHLSLDPVLAPLVSARPG
                     LRVPGAWDGFELAVRAIFGQQITVPAATKLLGRLVEAHGALLPTATRDVEGLSHLFPS
                     PGALARVDLTLLGMPNARAMAVNSLAQAISADPAIFSRGASLEETIAKLRSLPGVGEW
                     TAQYIAMRELREPDAFPAADIGLMRAMAAADCRRPSPAQLLSRAERWRPWRAYAALHL
                     WAAGIQHPVASGKPHEREAA"
     gene            complement(4078..4572)
                     /locus_tag="VGA15_01140"
     CDS             complement(4078..4572)
                     /locus_tag="VGA15_01140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9586336.1"
                     /translation="MADASAMDIASKTYGTVSAERQKQMSGLEFVQGLVDGTLPLNTI
                     ARTLGYDVTEAVSGRVVVTAEPSDIHLNPAGTVHGGLAATLLDSCMGLAIQSTLEKGV
                     SQTTLEFKISLLRPITPETGMIKAEGIVLSRGRRIGTAEGRVTDDKGRLLAHGTTTCL
                     IFQG"
     gene            4701..5063
                     /locus_tag="VGA15_01145"
     CDS             4701..5063
                     /locus_tag="VGA15_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEX9586337.1"
                     /translation="MIWIANLFVALVAALHVYFLILEMFLWTKPLGLKTFHNSIEKAT
                     DSAVLAANQGLYNGFLAAGLVWGLVHSTPGFAFQIKVFFLLCVITAGIYGAVTVSQRI
                     LLVQALPAAIALTLLWLA"
     gene            5218..5621
                     /locus_tag="VGA15_01150"
                     /pseudo
     CDS             5218..5621
                     /locus_tag="VGA15_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615142.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1071 a         1844 c         1697 g         1009 t
ORIGIN      
        1 atgcgcagat ttacaccacc accgacgggc gcgcgctcga caccatcgcg atctcaaggg
       61 aatacgaccg cgacgaggac gagggacgtc gcgccacgcg tatcggcgaa atgatcgagg
      121 acgtgctgga gggcaagttg cagctgcccg aagtggtggc gcgacggtcg aaccgcggca
      181 aggcgcggcc gttcgtggtc gagccggaag tgatcatcaa caaccagtgg tcggaccgct
      241 acaccgtgat cgaggtgtcc ggcctcgacc ggccgggcct gttgtatcaa ctgaccaccg
      301 cgatctcgaa actcaacctc aacatcgcct ccgcccatgt cgcgaccttc ggcgagcgcg
      361 cccgcgacgt gttttatgtc accgacctgc tcggcgccca gatcaacgcg ccgacgcggc
      421 aggccgcgat caagagcgcg ctgatccatt tgctcgccaa tgacgaagac gtggcgcagc
      481 ccgcggcgtg atcgacatcc atcaacgagc cgggacagac tgccgacaat gaaattgccc
      541 cgccaagccc gcgccgtcat cttcgatatg gacggcctga tgttcaacac cgaaaccctt
      601 taccgggacg cggtcgtcgc cgcagcggcc gaggccggcc atgatctgcc cctgccgttc
      661 tatctttcca cgatcggcat gtcgatcgag gcgacccgca tggcgttcag cgaacgctac
      721 ggaaaacagt tcgatttcga cggcttctgg gcggccgcct caacgctgta ttccgggatc
      781 gtcgaattgc agctctgcct gaaggcaggg gtggtcgaac tgctcgactt tctggatggc
      841 accagcctgt cacgcgccat cgccacctca tcgcgacacg aggacgtgca gcgtaacctc
      901 ggcgcgcaag gcctgcgtga ccgattccag acggttatcg cgcgcggcga ttatgcccgt
      961 ggcaaaccgc atccggagcc ctttctgaaa gcggccgagc ggctgggcgt cgagcctgaa
     1021 ctctgcgttg ccctggagga ttcctataac ggcgtgcggg ccgcgtccgg cgccgggatg
     1081 atgaccatca tggtgccgga cctgctgccg ccgacgcgcg agatggaaga actgtgcgta
     1141 tacatcgccc aagacctcca cgaggtccac agcctgatca gatcgcagcc agtcccttga
     1201 taagtctgcg cgccagctag aatctggtga tcggctccag cgcctcgccg gtgcgaagtg
     1261 cgcgtagcgg cgcggtgtcg caatagaccc gctcttcaat aatcttgccg gccttgatgc
     1321 gaaatttatc gacgccgcgc cagtcaaacc gcgcaccgtt gacgatacgg gtggtctgcc
     1381 attcgataat gaccacgtcg ccacgcgcgg tccagtcgag caattgccag acgaaatccg
     1441 gcgcggccgc ggtttgcacc tccatcaaac gattgagctc gcttcccgca acggttctgc
     1501 cgaccagcgg ggtgtgcagc agaccttccg gatgccacag ctcaacgaag gcctgaccat
     1561 cccgcgccgc ccatgccgcg gcaaaccgcg ttacaaaatc ctgaacctct gcatccgtca
     1621 tcgatcgtct ccatgttgtg tgcgaggaga cgctagggac attgtcggta tccgtctggc
     1681 gatttacgga cctgacaatt tggtctgctg gagagaggcc gtcgtccctg cgaaaaacaa
     1741 ttcgtcgtcc ccgcgaaggc ggggacccat aaccaccgac cttagttgtt acatcgaatt
     1801 gccgctccaa ctttgcaaaa tcgatacgcc aggggttatg ggtccccgcc ttcgcgggga
     1861 cgacgacggt gtttgcctag cgtggcgaat tctaaatcac gccaccctca agctgcgcgt
     1921 cttgatgcgg ctttcaacgc cgcgccttcg tgttgcagca gccatcgctt gcgctcgagt
     1981 ccgccgccat agccggtcag cgagccgtcg gcgccgatca cgcgatgaca gggcacgacg
     2041 acgctgatcg gattggagcc gttggcgtgg ccgaccgcgc gcacggcttt gggcattccg
     2101 agctgcgccg ccagcgcgcc gtagctcaac gtcgtgccgg caggaatggt ccgcagcgcg
     2161 gtccagacct tgcgctggaa cggcgtgccg gcgacgcgcc attttatccc gctcaggcga
     2221 tcaagatcgc ccttgaaata cccggtcagc gccgtcctga tatcatctgg cgcctgcgcg
     2281 tcttgcaaaa ccgtcgcgcc gtattgcagc cgcaagagct gcctcaggcg cagttcataa
     2341 tcctcccagt ccagcgcgca caacgcgccc tcggcatcgg tgacaagcaa ggcgataccg
     2401 atcggcgttt gcagccgatc gagaccgaat gtttcaggcg gcttcgcgtt catgcggttt
     2461 ccccgatgcg actggatgct ggattcctgc ggcccacaga tgcaaggccg cgtaagcgcg
     2521 ccacggccgc catcgttcgg cgcgcgacaa aagttgtgct ggcgacggac gccggcagtc
     2581 agccgcagcc atcgcgcgca tcaagccgat gtcggcggcg gggaacgcgt cgggttcgcg
     2641 cagctcgcgc attgcgatgt attgcgcggt ccattcaccg acgcctggaa gcgaccgcag
     2701 cttcgcaatc gtctcttcga ggctggcacc ccggctgaag atcgccggat cggcggaaat
     2761 cgcctgcgcc agcgaattca ccgccatcgc ccgcgcgttg ggcatgccga gaagcgtgag
     2821 atcgaccctg gcgagagccc caggcgacgg aaacagatgg ctgaggccct cgacatccct
     2881 cgtggccgta gggagtagcg cgccgtgggc ctccaccagc ctgccgagca attttgttgc
     2941 ggccgggacg gtgatctgct ggccgaagat cgcgcgcacc gcgagttcga aaccgtccca
     3001 ggcgccggga acgcgcaggc ccggccgcgc cgagaccagc ggcgccagca ccgggtcgag
     3061 cgaaagatgc tccccgatca tatcgggatc ggtggccaga tcgaacaccc gccgcacccg
     3121 cgcgataatc gccggcagcg cggccatgtc cggaaatcgc acggatacgc tcactctgtg
     3181 tttgtcggcc ggcgcgacgc tgatcacgcc gcagctctct ccaatcgaaa tcgtgcgccg
     3241 gtaaatattg tcagcgacaa tttcgatccc gggaatggcg cgcgcgctga gaaacgacag
     3301 catggcctcc caatcatagg gcggccgata ggcgagacgg accgacagtg cgccggcttc
     3361 gcgcttggtc cggtcgcgga aatgccgcag cgtcgccggc gaacgcccaa acatctgcag
     3421 aaaggtttcg ttgaagcgac ggacgctgtt gaagcccgac gccagcgcga cttcggccat
     3481 cggcaacgat gtctcgtgga tcaactgctt ggccagcaac acccgccgcg tctgcgccac
     3541 cgcgatcggc gacgccccga catgctggcg gaacagccgc cgcaattgtc gcgcgccgac
     3601 gcccagccga ttggcgaggc cctcgacatc cgcctcatcg aggccgccgg cttcgatcaa
     3661 ggcgagcgcg cgggacacgg tgttgctggt gccgcgccag aatgcgagat cgggcgaggt
     3721 ttccggccgg cagcgcaggc atgggcgaaa gccggcctcc tgggccgccg ccgcggaagg
     3781 atagaacaat acattttcga atttcggggt gcgggcgggg caaatcggcc ggcaatagat
     3841 gccggtggtt ttgacgccca cgaacagccg gccatcgaag cgggcatcgc gggtcgcgat
     3901 ggcgcggtag caggcggtgc ggtccatatc catcatcgaa gcatcgtcca ttgcagcaag
     3961 cttgtctcgc ggttttcgga catcattcat ggttgccggg ttgccggaaa ccctcgccat
     4021 ccaacgcgcc gcgctgcgtt gaacacaggt gcgccgggcg aaatccgggc ggcgccatca
     4081 accctgaaaa atcaggcacg tggtcgtgcc atgagcgagc aaacgccctt tatcatctgt
     4141 gacccgtcct tcggcggtgc caatccgccg gccccggctc aacacaattc cctccgcctt
     4201 gatcattcct gtctcagggg tgatcggccg caacagtgaa atcttgaact cgagcgtggt
     4261 ctggctgacg cccttctcca gcgtcgactg gatcgccagt cccatgcagc tgtcgagcag
     4321 cgtggccgcg agaccgccgt gcaccgtgcc ggctggattg agatggatgt cgcttggttc
     4381 cgcggtgacg acgacgcgcc cgctcaccgc ttcggtcaca tcataaccca aagtccgggc
     4441 gatcgtattg aggggcagcg tcccgtcgac gagcccctgg acaaattcca ggccactcat
     4501 ctgcttctgc ctctcggcac tgacggtccc gtaggtcttg ctggcgatat ccatcgcgga
     4561 agcatcggcc attgccgctg acctgtctcg cggttttcgg acatcatcat ttgccggaaa
     4621 cggccgcgcc gaccatcgcg ccgcgcgagc ggagcgtgct agcttgaccc ggcaaataac
     4681 tttgccgggg ggaacacaac atgatctgga tcgccaatct gtttgtggcg ctggtcgccg
     4741 cactacatgt ctattttctg attctggaga tgtttctctg gaccaagccg ctgggcttga
     4801 agacctttca taactcgatc gagaaggcga cggactccgc ggtgcttgcc gccaaccagg
     4861 ggctctataa cggctttctc gccgccggcc tcgtctgggg cctggttcac agcacccccg
     4921 gctttgcgtt ccagatcaaa gtgttctttt tgctctgcgt gatcacagcc ggtatctacg
     4981 gcgctgtgac cgtcagccag aggattctgc tggtccaggc attgcccgcc gccatcgcat
     5041 taaccctgct ttggctggcg taacttcgtt cacgccccga cccggcattg gcttgcaatg
     5101 ccacatccct tcatccagta tcttgcagta tcttgcgagc gatggcgtcc gccaaggtct
     5161 tcataggcct tgcggacaag atcatcgacc aatcatacat atgagggaag cagcgacatg
     5221 aggaacggca tttttcacct tgtcaccggg acggcggttg ccatcgcgct gccgcgtcgc
     5281 cggcatccgc gcaaaagaaa tacgacgtcg gcgcgaccga taccgagatc aagatcggcc
     5341 agaccgtgcc gttcagcgga ccagcctcgg cctatgccgg catcggcaag acacaagccg
     5401 cctacctcaa gatgatcaac gagcagggcg gcatcaacgg ccgcaagctc aatctgatcc
     5461 agtatgacga cgcctacagc ccgccgaaag cggtcgagca ggtgcgcaag ctggtcgaaa
     5521 gcgacgaggt cctgtttacc ttccagatca tcggcacgcc ctcgaacgcg gccgtccaga
     5581 aatacctcaa cggaaaaaag gtcccgcagc tgctcgcctc c
//
LOCUS       DASWGM010000031         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_556045,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000031 DASWGM010000000
VERSION     DASWGM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556045"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGA15_01155"
     CDS             <1..211
                     /locus_tag="VGA15_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HEX9586338.1"
                     /translation="RALDLIDALEARGSLKGYELMPAVRADLLRRLGRRDAARDAYRA
                     ATSATQLEPLRRLYARRLAELDCGS"
     gene            complement(310..1092)
                     /locus_tag="VGA15_01160"
     CDS             complement(310..1092)
                     /locus_tag="VGA15_01160"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9586339.1"
                     /translation="MKTFTPDSSIASDVIPSANYGDRNKGRLPDMILLHYTGMPDVEG
                     AIAKLCTVGTDVSAHYIVLEDGRIIQCVPEANRAWHAGEASWAGEEDINSCSIGVEIV
                     NRGHDWGYPDFPLRQIAAVIALCRGIILRRKVPSHRVLGHSDVAPARKKDPGEKFPWH
                     SLANSGVGHWVQPAPIVRSETLKLGTVSNDVRDLQQALARYGYPVPTSGHYDAATMEV
                     VTAFQRHFRPARIDGIADHSTLITLQALLESLSIDVKAFASR"
     gene            1555..>1881
                     /gene="mraW"
                     /locus_tag="VGA15_01165"
     CDS             1555..>1881
                     /gene="mraW"
                     /locus_tag="VGA15_01165"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX9586340.1"
                     /translation="MAAQQIPSGNQGLRSGLRGTSNRASPSFFCRKSLFGTKSPAMHR
                     HLRRNGPVRVQLSSALPDRARSRRMSPSAPRHIPVLGRQAIEMLSPREGGIYLDATFG
                     AGGYSRA"
BASE COUNT          345 a          554 c          612 g          370 t
ORIGIN      
        1 gcgcgcgctc gatctgatcg acgcgctgga agcccgcggt agtctcaagg gttacgagtt
       61 gatgccggcg gtccgcgccg atctgttgcg ccggctcggc cggcgtgatg cggcgcgcga
      121 cgcctatcgc gcggcgacat cagcgacgca gctggagccg ctgcggcggc tttacgcgcg
      181 gcgcttggcg gagctcgatt gcgggagctg atgcgagatc gcttccacgt atggatcgtc
      241 acggtcggga cataggcgag cggaagcgac gccgttcttc ggacggctat gcccggccat
      301 gaccatgccc tatcgcgacg caaaagcctt gacgtcgatc gagaggctct ccagcaacgc
      361 ctgcaatgtg atcagggtcg aatgatcggc gatgccgtcg atgcgggcgg gacgaaagtg
      421 acgctggaag gcggtgacca cttccatggt cgcggcgtcg taatgtccgc ttgtcgggac
      481 agggtagccg tatctggcca gcgcctgctg caggtcgcga acgtcgttgc tgacggtgcc
      541 gagcttgagg gtttcgctgc gcacgatcgg cgccggctgc acccaatggc cgacgcctga
      601 attggccagc gaatgccacg gaaatttctc gccggggtct ttcttgcgcg ccggagcaac
      661 gtccgaatgc ccgagcacgc gatgggacgg caccttgcgg cgcagtatga tgccacggca
      721 caacgcaatc acggcggcga tctggcgcag cggaaaatca ggatagcccc agtcgtggcc
      781 gcgattgacg atctcaacgc cgatcgaaca ggaattgatg tcctcttcgc cggcccagga
      841 tgcctcgccg gcatgccagg cgcggttggc ctccgggacg cactggatga tgcggccgtc
      901 ttccagcacg atataatgcg ccgaaacatc ggtgccgacg gtgcagagct tcgcgatcgc
      961 gccctccaca tccggcatgc cggtgtagtg cagcagaatc atgtcgggca accggccctt
     1021 gttgcgatcg ccataattcg ccgacgggat gacgtcggaa gcgatcgatg aatcgggagt
     1081 gaaggtcttc atgttcgcgc tggcccttgg gatcggcagg gcacgttgac gcttcgaatc
     1141 aaaaccacct gatgcggacg aacctgacgg cataggatag ctcaaaccgg gcagtagaac
     1201 ggtacatttt tttgggaacg aatctcgttt aatatttcct caccgtgccg tccgcgcaat
     1261 ggtgagccgg tcgccgcctc gcattttgac cgggtgtgtg cggcgggcat caccggtcgt
     1321 atcgtccatc gatcctgata tcaaataccg atgttccgat tcgttgggac gatttgggca
     1381 agcaggcatg ggctgggcgt ggccagatcg aggcatggaa ctcgccggat cttgcgcgag
     1441 ggcgattgta tcggtcccat caaccctttc ttaatgctaa cggccgttac taagtggtga
     1501 agcgctggaa ccggttttgg gggacggccg aggccctatg acgttcgaaa tcccatggca
     1561 gcccagcaaa ttccatcggg aaatcagggt ttgcgatctg ggctgcgcgg gaccagtaat
     1621 cgtgcctcgc caagcttttt ttgccgaaag tcgcttttcg ggacgaaaag cccggcgatg
     1681 cacaggcatt tgaggcgcaa tgggcccgtt cgcgtccaac tttcatctgc tttgccggat
     1741 cgcgcccgga gccggcgcat gagtccgtct gccccacgtc atattcccgt ccttggccgc
     1801 caggcgatcg aaatgctgag cccccgcgag gggggcatat atctcgatgc cacctttggg
     1861 gccggcggtt acagccgggc c
//
LOCUS       DASWGM010000032         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1804375,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000032 DASWGM010000000
VERSION     DASWGM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804375"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGA15_01170"
     CDS             <1..433
                     /locus_tag="VGA15_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9586341.1"
                     /translation="LIQVFLNLVKNAAEAVVDLGTDAEIQLTTAFRPGVRLSVPGKKS
                     RVSLPLEFCVKDNGPGVPEDLLPNLFDPFVTTKPTGSGLGLALVAKIVGDHGGIIECE
                     SQPRKTTFRVLLPMFNSTKHFDQSNREHVSGTPLHASQGPR"
     gene            443..>1672
                     /gene="ntrC"
                     /locus_tag="VGA15_01175"
     CDS             443..>1672
                     /gene="ntrC"
                     /locus_tag="VGA15_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX9586342.1"
                     /translation="MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVS
                     QGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIVMSAQNTFMTAIRASERGAYEYL
                     PKPFDLKELIAIVGRALAEPKERVASHADEAEFDSIPLVGRSPAMQEIYRVLARLMQT
                     DLTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFT
                     GANTRASGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRI
                     VAASNKDLRILIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPDLIRHFFALAEKDGL
                     PPKKLDALALERMKQHRWPGNVRELENLARRLAALYPQDVITGSVIDGELAPPAVTSG
                     GSVQHGVDNLGGAVEAYL"
BASE COUNT          316 a          512 c          532 g          312 t
ORIGIN      
        1 gttgattcag gtgtttctga atctggtgaa gaacgccgcc gaggccgtgg tcgatctcgg
       61 taccgacgcg gagattcaac tcaccaccgc attccgtccg ggcgtccggc tgtcggtgcc
      121 ggggaagaaa tcgcgggtgt cgctgccgct cgagttttgc gtcaaggaca acggccccgg
      181 cgtgccggaa gatctgttgc cgaacctgtt cgatccgttc gtcacgacca aaccgaccgg
      241 cagcgggttg ggcctcgcgc tggtggccaa gatcgtcggc gaccacgggg gcatcatcga
      301 atgcgagtcc caaccgcgga agactacgtt ccgtgtactg ctgcccatgt tcaacagcac
      361 caagcacttc gaccagagca atcgcgagca cgtttcgggg acgccgttgc atgcctcaca
      421 gggcccaaga tgaggaataa caatgcctgc aggtagtata cttgtcgccg acgatgatac
      481 cgccatccgt acggttctaa accaggccct gtcgcgcgcc ggatacgagg tgcgcctgac
      541 cggaaacgcg gcgacgctgt ggcgctgggt cagccagggc gagggcgatc tggttatcac
      601 cgacgtcgtg atgcccgacg agaacgcatt tgatctgctg ccgcggatca agaagatgcg
      661 gccgaacctt ccggtcatcg tgatgagcgc gcagaacact ttcatgacgg cgatccgcgc
      721 gtccgaacgc ggcgcctatg aatatctgcc aaagccgttc gacctgaagg agctgatcgc
      781 cattgtcggc cgcgcgctgg cggagccgaa ggagcgggtt gcaagccacg ccgacgaagc
      841 cgagttcgat tcgattccac tggtcggccg gtcgcccgcg atgcaggaaa tctatcgggt
      901 gctggcgcgg ctgatgcaga ccgacctcac ggtgatgatc tccggcgagt ccggcaccgg
      961 caaggagctt gtcgcacgtg cgctgcatga ctacggcaag cggcgcaacg gcccgttcgt
     1021 cgccgtcaac atggcggcta tcccacgcga tctcatcgaa tcggaactgt tcggccatga
     1081 acgcggcgca ttcaccggcg ccaacacccg cgcctcgggc cggtttgagc aggctgaggg
     1141 aggtactcta tttctcgacg aaatcggcga catgccgatg gaggcgcaga cccgtctgtt
     1201 acgggtcctt cagcaaggcg agtacaccac cgttggcggt cgtacaccga tcaagaccga
     1261 cgtgcgcatc gtcgctgcga gcaacaagga tctgcgcatc ctgattcagc agggtctgtt
     1321 tcgcgaggat ctgttctttc gcttgaacgt ggttccgttg cggctgcctc cgctgcgcga
     1381 gcggatcgag gatttgcccg atttgatccg gcattttttc gcgctggccg agaaggacgg
     1441 actgccgccg aagaagctcg atgccctggc gttggaacgc atgaagcagc atcgctggcc
     1501 gggcaatgtg cgcgaactcg aaaacctcgc gcgccggctc gcggcattgt atccgcaaga
     1561 tgtgatcacc ggctctgtca tcgatggcga gctggcgcct cccgccgtga cctcgggcgg
     1621 cagcgtccag cacggagtcg acaatctcgg tggcgccgtc gaagcctatc tt
//
LOCUS       DASWGM010000033         7136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_694584,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000033 DASWGM010000000
VERSION     DASWGM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694584"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VGA15_01180"
     CDS             <1..239
                     /locus_tag="VGA15_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HEX9586343.1"
                     /translation="QVFLKSIPLLILAGLGYLVFALAINVVMRVYLLRDLWVRVLAST
                     IVLDIEAAANVSARGEMANALGEGFADGLDVAGF"
     gene            259..1374
                     /locus_tag="VGA15_01185"
     CDS             259..1374
                     /locus_tag="VGA15_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX9586344.1"
                     /translation="MDSGPQNASASPGAGGSAIYFDGTSSRRRLVTLAFNDGLELQED
                     GHTLAVWSFADIRRADSPSGALRVTCLTAPALARLEIRDAAVATKLISRCARLDENIP
                     GRHGVAKIVGWSLAATVSIVAVVLFGVPLAADRLAPLVPQALERRLGDVAEGQVKTLF
                     GGKTCNNTSGQAAFTKLVDAVRESTGLDTSIQAQVLATPVPNAFALPGGRVYLFSGLL
                     AKAENADEIAGILAHELGHLRHRDGTRNLIYNGGTSFLIGLLFGDITGSGALIFASRS
                     LVTASYSREAEQNADTFAIDAMHRLGRSPKPMGELMFRVTGKEGDTSLSILSNHPLTE
                     DRVARMSHEDRPPSGPPLLTPSEWASLKAICSSDSKI"
     gene            complement(1375..2187)
                     /locus_tag="VGA15_01190"
     CDS             complement(1375..2187)
                     /locus_tag="VGA15_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2927 domain-containing protein"
                     /protein_id="HEX9586345.1"
                     /translation="MSANYRALLLRIFAVALAGIAFDGGAWLPAASAEIPAIASRQRA
                     EKKTFTDSEITDGFFKTAFGAEYHLAGRVDRIRKYDAPVRVFAEGNRPDRKAQLAKIV
                     ADIAERIQHLDIAMVDTSDGANVLVKLVRDRDLYRTITTFYGSERAREIRTSLDPQCL
                     SGFRKNEKFEIEHSDVILTVDNGDFIFFDCGYEELLQSLGPINDTSSVPWTMFNDNVS
                     MGFFDVYDQYILNILYDPRVKAGMTVAEVKAVLPEVLADVRIWVRKINNLTE"
     gene            complement(2298..2900)
                     /locus_tag="VGA15_01195"
     CDS             complement(2298..2900)
                     /locus_tag="VGA15_01195"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9586346.1"
                     /translation="MTRAFASLFAAMILLAGAGHAYAQGFPGYVQGFPSFDSRDIMGG
                     GPNFSVGASSPIPRQTVRFESRYTPGTILIDTAERRLYLVLPNGQALRYGIGVGRDGF
                     RWGGVHRISAKKEWPSWTPPSQMLARRPDLPRHMAGGIDNPLGARAMYLGSTLYRIHG
                     SNEPETIGQAVSSGCFRMTNDDVADLYDRVFVGTTVVIKN"
     gene            3126..4670
                     /locus_tag="VGA15_01200"
     CDS             3126..4670
                     /locus_tag="VGA15_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX9586347.1"
                     /translation="MNGAESLVRTLVAGGVDVCFANPGTSEMHFVAALDRVEGMRCVL
                     GLFEGVVTGAADGYFRMKGTPASTLLHLGPGLANGLANLHNAKKANSGIVNIVGQHAV
                     YHIGFNAPLTSDIEGLARPMSAWARTSPDAKSVAKDGAAAIAAAKSAPPQIATLILPA
                     DTAWNEADGIAQVPAESQRVSYSSQAVDNAAKVLRDGAQTLLLLTGSALTEHGLALSA
                     QIAGKTGCKVLGQTYNPRMARGRGRFSINRIPYVIEQALPILKDFRHIVLVEANDPVA
                     FFAYPNKPSMLKPEGCEVHRMTSGGENSVAALEALASALGAKPADAKPQALVELAKPT
                     GALTHASIAQAIAMAIPENAIVVDESITTGRGFFPPTAAAKPHDWLQNMGGSIGFSTP
                     VATGAAVACPDRKVICMVGDGSAMYTLQSLWTQAREGLNVLTIVFANRIYQILRGEFD
                     GVGAGEPGQRAMDMLKIDRPALDWVALAKGMGVAGRAVTTADEFVNALAEGIAEQGPR
                     LIEVQM"
     gene            4747..5262
                     /locus_tag="VGA15_01205"
     CDS             4747..5262
                     /locus_tag="VGA15_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586348.1"
                     /translation="MQAALNKVLGALGEFYARETHLFAKDLGERTLTHRLAVRLEKQF
                     PGWEVDCDYNRLGERTLRLPHGTIVSTDDQLGKSVYPDIVVHQREVPNNLLAIEVRKA
                     ANHQPPEHDRHKLRAMTDPHLWFAFRIGLLLTLGKKNVTSAEIYVGGLPDQPLSGWLA
                     GRLKEAGLGAA"
     gene            5471..5695
                     /locus_tag="VGA15_01210"
     CDS             5471..5695
                     /locus_tag="VGA15_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586349.1"
                     /translation="MARKNPAAGPSTDGGKQVRARPHDSGSAANETIDGLDATTEALR
                     HAAEDTPSGAAPEDVEKTPVFDRAGLAPRI"
     gene            5695..5847
                     /locus_tag="VGA15_01215"
     CDS             5695..5847
                     /locus_tag="VGA15_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586350.1"
                     /translation="MAKKQRGEIVERTTEARRAEPGSSVLSLSVVSLCLAVLILAGVW
                     YAFFRT"
     gene            6092..6220
                     /locus_tag="VGA15_01220"
     CDS             6092..6220
                     /locus_tag="VGA15_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586351.1"
                     /translation="MTHWHDPMVDTPETDGAVYMTGWTISGLAVLGAIVAVWLLGI"
     gene            7052..>7136
                     /locus_tag="VGA15_01225"
     CDS             7052..>7136
                     /locus_tag="VGA15_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586352.1"
                     /translation="MNPGDPDAPRRFRQIVAAYAILRDAEHR"
BASE COUNT         1375 a         2137 c         2238 g         1386 t
ORIGIN      
        1 cgcaggtgtt tttgaagagc atcccgttgc tgatcctggc gggactgggt tatctggtgt
       61 tcgcgctggc gatcaacgtg gtgatgcgcg tctatctgtt gcgcgatctc tgggtgaggg
      121 ttctcgcatc gacgatcgtc ctcgacatcg aggcggcggc gaatgtttcc gcaaggggcg
      181 aaatggccaa cgcgctgggc gagggctttg ccgacgggct ggatgtcgcc ggcttttagc
      241 gcatgagcat gtatcgcaat ggatagcggg ccgcaaaatg cgtcggcgtc gccgggagcg
      301 ggcggttccg cgatttattt cgacgggacg tccagccgcc gccgtttggt gacactggcg
      361 ttcaatgatg ggcttgagct ccaggaggat ggacacacgc ttgcggtatg gtcttttgcc
      421 gatatccgcc gcgccgacag tccgtccggt gcgctccgtg tgacctgcct gacggcaccg
      481 gcgctggcgc ggcttgaaat ccgcgatgcg gcggttgcca cgaaactgat ctcccgctgc
      541 gcccggctcg acgagaatat tcccggccgg cacggcgtcg cgaaaattgt cggctggtca
      601 ttggcggcga cggtttcgat cgtcgccgtg gttctgttcg gtgttccgct cgccgcggac
      661 cgtctggcgc cgctggtgcc gcaggcattg gaacggcggc ttggcgatgt cgcggaaggg
      721 caggtcaaga cgctgttcgg cggcaaaact tgcaataaca catcgggaca ggctgccttc
      781 accaagctcg tcgatgccgt tcgcgagtca actggcctgg atacgtcgat ccaggcccag
      841 gtgctggcta ccccggttcc caacgcattc gcgctgcccg gcggcagggt gtatttgttc
      901 agcgggctgc tggccaaggc cgaaaacgcc gatgaaatcg ccggcatcct ggcccatgag
      961 cttggtcatc tcaggcatcg cgatggcacg cgcaatctga tctacaatgg cggaacgtcg
     1021 tttctgatcg ggttgttgtt cggggacatc accggctccg gcgccttgat ttttgcttcg
     1081 cgctcgctgg tgaccgcgtc ctattcgcgc gaggcggagc aaaatgccga tacatttgca
     1141 atcgatgcga tgcaccggct tggccgctcg ccaaagccga tgggcgaatt gatgtttcgc
     1201 gtcaccggga aagaaggcga cacgagcctg tccatcctgt cgaaccatcc actgacggag
     1261 gaccgcgtgg cgcggatgag ccatgaggat cggccgccga gcggtccgcc gctgctgacg
     1321 ccgtcggaat gggcgtcgct caaggcaatc tgcagctccg attccaaaat ctgatcattc
     1381 agtgagatta ttgatcttcc gcacccagat gcggacgtcg gccagcacct cgggcagcac
     1441 cgccttgacc tccgcgacgg tcatgccggc tttgacgcgt ggatcgtaca gaatgttgag
     1501 gatgtactgg tcatagacgt cgaagaaccc catcgacaca ttgtcgttga acatggtcca
     1561 gggcaccgaa gaggtatcgt tgattggccc cagcgattgc agcagttcct catatccgca
     1621 gtcgaagaag atgaaatcgc cgttgtcgac cgtgaggatc acgtcggagt gctcgatttc
     1681 aaatttttcg ttcttgcgaa aaccggacag gcattgcgga tcgagcgagg tgcgaatctc
     1741 gcgcgcccgt tcgctgccgt agaacgtcgt gatggtgcga taaagatcgc ggtcgcgcac
     1801 cagcttgacc agaacattgg cgccgtcgct ggtgtccacc atggcgatgt cgagatgctg
     1861 aatccgctcg gcgatgtcgg ccacaatctt cgccagttgc gcctttcgat ccggacggtt
     1921 gccctcggcg aagacccgca ccggcgcatc gtattttctg atccggtcga cccggccggc
     1981 aaggtgatac tcggcaccga aggcggtctt gaaaaatccg tcggtgatct cgctgtcggt
     2041 gaatgtcttc ttttcagcgc gttgacgcga cgcgatggca ggaatttcgg ccgacgcggc
     2101 tggcagccag gcaccgccgt cgaacgcgat gccggccaaa gcgaccgcga atatccgcaa
     2161 aagcaaagct cggtaatttg cgctcattgc aacgacgctg ccgcaaccat ggcgccgacg
     2221 caatctctga atgcaggcta cgcatcgggt tccacgtcgc gctgtggagc gcgccggttc
     2281 ccttgaccgg cgcccgtcta gtttttgatg acaaccgtcg ttccaacgaa gacgcggtcg
     2341 tagagatcag ccacgtcgtc gttggtcatg cggaagcagc ccgacgatac cgcttggccg
     2401 atcgtctccg gctcgttcga accgtgaatg cgataaagcg tcgatccgag atacatggcg
     2461 cgggcgccga gcgggttgtc gatgccgccg gccatgtgcc gcggcagatc gggccgccgc
     2521 gccagcatct gcgagggcgg tgtccagctc ggccattcct tcttggctga tatgcggtgg
     2581 acgccgcccc agcgaaagcc gtcgcggccg acgccgatgc cgtaccgcag cgcctggcca
     2641 ttgggcagca cgaggtaaag ccgccgctcg gccgtatcta tcaaaatagt gccaggcgtg
     2701 tatcggcttt caaatctcac ggtctgccgc gggatcgggc tcgatgcacc gacactgaaa
     2761 ttggggccgc cgcccatgat gtcgcgagag tcaaaactcg ggaaaccctg gacataaccc
     2821 gggaaaccct gggcatacgc gtggccggcg ccggccagca aaatcatcgc cgcaaacaac
     2881 gaagcaaaag cccgggtcat attcttcctc gcgaaaaaaa tccgtgcctg cgagacaggc
     2941 cataattggc gcgatttcgc aagcaaagag cccgtgagcc ctgcgatttt catacgctgc
     3001 ggtaaaaaag ccatcacccc ctgagcgatg gctccaatgc ccgcgaaacc tttgacgaaa
     3061 ggcgctgggg atgattgatc tcccaagagt gctttttgag aagcgtcgcg gacgggagtg
     3121 gaataatgaa cggtgcggaa agtctggtgc ggaccttggt cgcgggcggc gttgacgtct
     3181 gcttcgccaa tcccggcacc tccgaaatgc atttcgtcgc agcccttgat cgggtggagg
     3241 gcatgcgctg cgtgctcggc ctgttcgagg gtgtggtgac gggtgcggcg gacggctatt
     3301 tccgcatgaa ggggacgccg gcatcgacgc tgctgcacct cggaccgggg ctggccaacg
     3361 gtcttgccaa tctgcacaac gccaagaagg cgaattccgg catcgtcaac atcgtcggcc
     3421 agcatgccgt ctatcacatc ggattcaatg cgccgctgac ctccgacatc gagggcctgg
     3481 cgcggccgat gtcggcgtgg gcgcgcacct cgccggacgc caaatcggtc gccaaggacg
     3541 gtgctgcggc cattgccgcg gccaaaagcg cgccgccgca gattgccacc ctgatcctgc
     3601 ccgccgacac cgcatggaac gaggccgacg gcattgcgca ggtgccggcg gaatcccagc
     3661 gcgtcagcta ttcgtcgcag gccgtcgaca atgccgcgaa ggtcttgcgc gacggcgcgc
     3721 agacgctgct gctgctcacc ggcagcgcgc tgaccgagca cggactggcg ctatcagcgc
     3781 aaatcgccgg caagaccggc tgcaaggtgc tgggccagac ctacaatccg cgcatggcgc
     3841 gcgggcgggg gcggttttcg atcaaccgga ttccttatgt gatcgagcag gcgctgccga
     3901 tcctgaaaga ttttcggcac atcgttctgg tcgaagccaa cgacccggtg gcgtttttcg
     3961 cctatccgaa caagccgagc atgctgaaac cggagggctg cgaggttcac cggatgacct
     4021 caggcggcga aaactcggtg gcggcgttgg aagcattggc gtccgcgctg ggcgccaagc
     4081 cggccgacgc aaaaccgcag gcgctggtcg agctggcaaa gccgacgggc gcgctgaccc
     4141 acgcctcgat cgcgcaggcg atcgcgatgg ctatcccgga aaatgccatt gtggtcgatg
     4201 aatcgatcac aacggggcgc gggttctttc cgccgaccgc cgcggccaag ccgcacgact
     4261 ggctgcagaa catgggcggc tcgatcgggt tttcgacgcc ggtcgcgacc ggcgccgcgg
     4321 tggcgtgccc cgaccgcaag gtgatttgca tggtcggcga cggcagcgcg atgtacaccc
     4381 tgcaatcgct gtggacgcag gcccgcgaag gcctcaacgt tctgaccatc gtgttcgcca
     4441 accgcatcta tcagatcctg cgcggcgaat tcgatggtgt tggcgccggc gaaccgggac
     4501 agcgcgcaat ggacatgctg aagatcgacc gccccgcact cgactgggtt gcgctcgcca
     4561 agggcatggg cgttgccggc cgcgccgtca ccactgccga cgaatttgtc aacgcgctgg
     4621 cggagggcat cgccgagcag ggaccgcggc tgatcgaagt acagatgtga ggctgagggc
     4681 gaccgtcgaa ccgccgcgag tgccgcggct taggaagttt ttgacggaga cgacatgcaa
     4741 gccgagatgc aagccgcgtt gaacaaggtt ttgggcgcgc tcggggaatt ctatgcgcgc
     4801 gagacccacc tgtttgcgaa ggatctcgga gagcgcacgc tgacgcaccg gctggcggtg
     4861 cgtctcgaaa agcagttccc gggctgggag gtcgattgcg attacaaccg gctgggcgag
     4921 cggacgctgc ggctgccgca cggaacgatt gtctccaccg acgatcaact cggaaaatcc
     4981 gtttatccag atatcgtggt gcaccagcgc gaggttccca acaatctcct cgccatcgag
     5041 gttcgcaagg cggccaatca tcagccgccg gaacatgacc ggcacaagct gcgcgcgatg
     5101 accgatccgc atttgtggtt cgccttccgg atcggactgc tgctgacgct tgggaagaag
     5161 aacgtcacct cggcggagat ttatgtcggc ggcctgcccg accagcccct atccggctgg
     5221 ctggccggac ggctgaagga agcggggttg ggcgcggcgt agcgcaggca agtggctgcg
     5281 cctcccggat cgatcatgcg cacggaccgt gccgccgcgt gagcagatga tgcggaccgc
     5341 gccagccccg ccatctcgct gatctgatac gctgatctga taggcgccgg gtttgcaaaa
     5401 aagggacagt tggcggtgga acagactacc tcccggtaga ttggtgtcgc gacctccagg
     5461 gagacaagcc atggcacgga agaatccagc ggccggtccg agtacggatg gcggcaaaca
     5521 ggttcgagcc cgaccgcacg attccggcag tgcggctaac gagaccattg acggattgga
     5581 cgccacgacc gaggcgctac ggcacgccgc cgaagatacg ccctcgggcg cagcgccgga
     5641 ggatgtcgag aaaacgccgg tgtttgaccg ggccgggctg gcgccgagaa tctgatggca
     5701 aagaaacaac gcggcgagat tgtcgaaagg accacagagg cgcgtcgggc cgaacccggc
     5761 tcgtcagtgc tttcgctgtc ggtcgtcagc ctctgccttg ccgtcctcat tctcgctggc
     5821 gtctggtacg cgttctttcg cacctgatgg aaccgacagg ccagcaccgc gattatcgta
     5881 tttgaacctt accaccgctg gcggagacgt acctaccaga ggcaatctcc ggagtctttt
     5941 ccgtttcgat agaatccaaa cggggctcca gattattgat ttgacgcgtt ttcttgacgc
     6001 gaaccggtct ccaccccgga tcaagtccgg ggcaggcccc cggatcaagt ccgagggcat
     6061 gcttcgcggg aaaacgcttt aggaggctga tatgacccat tggcatgacc cgatggtgga
     6121 cacgcccgaa accgacggtg cggtttacat gacgggatgg acgatttccg gcctggccgt
     6181 acttggcgcc atcgttgcgg tttggcttct tggaatttga gccggaacga ttttgcgcca
     6241 catcgtgagg ccaccacgca aaccgcgtgc atggagaagt gcacgccgct tcgtactttt
     6301 gaaaaagtat ctgttttatt gaccgttctg gtggacgcac aagggatcga accttggacc
     6361 tctcccgtgt gaaggaattt tacggccttt gaaatcactc atgttttctc ctttcgttgg
     6421 tagattccac ggattttcca cgcttttgct ggtccggggc gccgatgctt accagcgcaa
     6481 ccggcaccac ctattcgtag tcgtcgaaca tggcaagtaa ctcgtccaag ccggtatcga
     6541 cccgagcata ccaagcatcc atagcagggg ccgtgttggc ctgcgcggca gcgtgcctta
     6601 tctccgaatt aaatctgact aaccactcaa ccaagaggcc catttgttga tgaccctgtt
     6661 tgagtttcgc gaacctctcg agaacctttt ggtattgctc ctcgtgtccc cggcgagcga
     6721 ttcctctgtc aatacgggac ccaacgtact caaccgtggt tcgtattctt tggaaagagc
     6781 ttgcgtaatc ggaaaccaaa tcgtccaata tctcgaggac gcccactatg aactcggcat
     6841 tgatgcggtg cgccgttgct tttctaaaat ttgtgagcgc atacgctatt ttaaacaacg
     6901 agttgggcat cgcccgcagg tcggcactcc actcctcttc gcagaaattg cctgcgaaaa
     6961 cactttacga tctgctccgt gttcgtcggg gtgacacgct gagacgctcc aaagtgcatt
     7021 ccgcaacgcc gcaaaggcga accatcctga cctgaatccc ggcgatccgg atgcacccag
     7081 gcggttcagg caaattgttg ccgcgtatgc gatccttcgc gacgcggagc atcggg
//
LOCUS       DASWGM010000034         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2218943,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000034 DASWGM010000000
VERSION     DASWGM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218943"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VGA15_01230"
     CDS             complement(<1..1016)
                     /locus_tag="VGA15_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX9586353.1"
                     /translation="MKLRDLIAGAEVQRVNGDADVEITGLSYDSRRVNRGDLFFSTAR
                     DLNQNRANVDEALNRGARAVVVKDGDGVAARPAVTIVECGRPRLVMGVAASHFFNAPS
                     RRMDLIGVTGTSGKTTTTYLLASIFEAAGIPCGIIGTIGTFVGSRTLYSGLTTPESID
                     FEGALAEMSRTGVRAAAAEISSIGLDEGRVEELNFRACVFTGLGRDHLDYHGNLENYF
                     AAKLHLFTHLLPRSRHPEPVAILRGDDPYGNRVFASVTGHKLSFGLDAALDVHPISFE
                     AGLAGIRASVYAAGRTFEVHSSLLGKINLLNILCACALSVALGIETDAVVEGVRRCPG
                     APGR"
     gene            complement(1013..>2487)
                     /locus_tag="VGA15_01235"
     CDS             complement(1013..>2487)
                     /locus_tag="VGA15_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEX9586354.1"
                     /translation="IFDSPLALKNAEPGARLELTIDSSIQSMAESYLAAQIEKSGAKR
                     GSAVVLDPFTGEVLALANDYADRRADDGRLHNAAVQDAFEPGSTMKGILGAVALQDHV
                     IDTTREIYCENGQWRVGGRAIHDDSPHQRLNLGGIIEVSSNIGAAKIALALGAQRFYQ
                     GLAAFGLGRKSGVDLPGEAAGLIRAPSRWRQIELADHGFGQGLAVTPIQLATAYAAIA
                     NGGIVMRPFVVRAAYDADGREILRHTPQALRRAVSPDVAHTMNQLLRGAVDGPEGTGR
                     LARVADFTVAGKTGTAQMVNPATGTYYQSRLVASFVGFVPAEDPRMVILVVLYDVPHG
                     HFGGLFAAPVFSEIAGDALAHLEVVPPAPSYETASLLPFGVWDNSDQHDEGAPESRSD
                     SSVNTGFASVSAASGIVPEFRGLSLRAALDLARSRRIDVEISGSGYVVRQKPGPGAVL
                     ARPPAELTLMEKSEARPLPALTGMISHQHRDHLNRVRAAR"
BASE COUNT          488 a          793 c          753 g          453 t
ORIGIN      
        1 aacctgccgg gggcccctgg gcatcgccgc acaccctcca ccaccgcatc cgtttcgatg
       61 cccagggcca ccgacagggc gcaggcgcac aaaatgttaa gcagattgat tttgccgagc
      121 agcgaagagt gcacttcaaa cgtacgtccc gcggcataca ctgaagcgcg gatcccggcg
      181 agtcccgcct cgaagctgat tggatgcacg tcgagagcgg cgtccaggcc gaagctgagc
      241 ttgtgtccag tgacgctcgc gaacacgcga ttgccatagg gatcgtcgcc gcggaggatc
      301 gcaaccggct caggatggcg gctcctgggc aacaggtgtg tgaataggtg caactttgca
      361 gcgaagtagt tttcaaggtt gccgtgataa tcgagatggt cccgaccgag gccggtgaag
      421 acacaggcgc gaaagttcag ttcctctacc cggccctcat ccaggccgat cgaggaaatc
      481 tccgccgctg ccgcgcgcac gcccgtccgc gacatctcgg ccaacgcgcc ttcgaaatcg
      541 attgattctg gcgtcgtgag gccgctgtat agcgtgcggc tgccaacgaa ggtcccgatc
      601 gtaccgataa tgccgcatgg aatgcctgcc gcttcgaaga ttgacgcgag cagatacgtt
      661 gttgtggtct tgccgctggt gccggtcacg ccgatcagat ccattcgacg gctgggcgca
      721 ttgaaaaaat gcgacgcggc cacgcccatc acaagacgtg gccggccgca ttccactatg
      781 gtcaccgcgg ggcgcgccgc cacaccatcc ccatccttca ccaccactgc acgcgctccg
      841 cggtttaatg cttcatcgac attggcgcga ttctggttaa ggtcgcgcgc cgttgaaaaa
      901 aagagatcgc cgcgatttac gcggcgggag tcatagctca ggccagtgat ctcgacatcg
      961 gcatcaccgt tgacgcgttg tacttcggcg ccagctatca agtcgcggag tttcatcgtg
     1021 ctgctcgtac gcgatttaag tggtcccgat gctgatgcga aatcatcccc gtcagagcag
     1081 gcaatggccg cgcctcggac ttttccatga gcgtcaactc ggccggcgga cgcgcgagca
     1141 ctgcgccagg tccgggtttc tggcggacga cgtagccgga cccgctgatc tcgacatcga
     1201 ttcggcggct gcgcgccaag tcgagcgccg ccctaagact gagcccgcga aactctggaa
     1261 cgataccgct tgcggccgac acggatgcaa accccgtatt gacggatgaa tccgatctcg
     1321 actcgggtgc cccttcgtcg tgttggtccg aattgtccca tacgccgaag gggagcaggc
     1381 ttgccgtctc atacgatggc gctggtggaa caacctcgag gtgtgcgagc gcatccccag
     1441 cgatctcact gaaaaccgga gcggcaaaaa gaccgccgaa gtgtccatgc ggaacgtcgt
     1501 acagcactac gagaatcacc atccgtgggt cttcagcggg aacgaatccg acgaacgacg
     1561 ctacgagccg gctctggtag tacgtgccgg tggcaggatt aaccatctgc gcggtgccgg
     1621 tcttgccggc cacggtgaaa tcggcaacgc gcgcgagccg tccggtaccc tcaggtccgt
     1681 cgaccgcgcc gcgcagcaac tgattcatcg tatgtgccac gtcgggagag accgcgcgcc
     1741 gaagcgcctg cggagtatga cgcagaatct cacggccgtc ggcgtcgtaa gccgccctta
     1801 ccacgaacgg tcgcatcacg atgccgccgt tggcgatcgc tgcataggcg gtcgcgagct
     1861 gaatcggcgt aactgcgagc ccctggccga aaccgtggtc ggcaagctca atctggcgcc
     1921 acctcgacgg cgctcgaatc aggccggcag cctcgcccgg aagatcgacc ccgctcttgc
     1981 gccccaggcc gaacgcggcc agtccctggt agaagcgttg cgcgccgagc gcaagcgcga
     2041 tcttggcagc gccgatattc gacgagacct cgataatgcc gccaaggtta agcctttggt
     2101 gtggactgtc gtcgtggatc gcgcgtccgc ccacacgcca ttggccattc tcgcaataaa
     2161 tctcgcgagt cgtgtcgatg acgtggtcct gaagtgcgac ggcgccaagg attcccttca
     2221 tcgtggagcc gggctcgaat gcgtcctgca ccgcggcgtt gtgcagccgg ccgtcatcgg
     2281 ctcgacggtc tgcatagtcg ttagcgagcg cgagcacctc accggtaaac gggtcaagca
     2341 ccacggcact cccgcgctta gccccgcttt tctctatctg cgcagccaga taactctcgg
     2401 ccatggactg gatcgacgaa tcgatcgtca gttcgagccg cgcaccgggt tcggcatttt
     2461 ttaatgccag aggcgaatcg aagatcg
//
LOCUS       DASWGM010000035         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1249033,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000035 DASWGM010000000
VERSION     DASWGM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249033"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VGA15_01240"
     CDS             complement(<1..675)
                     /locus_tag="VGA15_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX9586355.1"
                     /translation="MFDLPKYGDGVCLARLADLLDAVEVDRARLSRISVVVTGSNGKG
                     STAAFCAGIAHACGLRTGLFTSPHLYRFNERFQVDGVPIGDDDLARLVARIGSAITEI
                     AQRSNQRFGAFEAMFALACLHFSERQCDFAVFEAGIGGRYDPVRLVGALATCVTSADY
                     EHVELLGNSLELIASDKSDACAAGGTIVYGENCRSLRPHLTEYNRTRGIASLFVRDEI
                     GIGGETV"
     gene            complement(668..1672)
                     /locus_tag="VGA15_01245"
     CDS             complement(668..1672)
                     /locus_tag="VGA15_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586356.1"
                     /translation="MRGLEKFRSGKPGSYPDQSIYAEYACAEFGLAFQDIDGGTGLVF
                     SVASKTKTIHFGAGRCSWYPQNNATASTLASDKYFANTILERAGAATLGGEYFFLHER
                     HRARRAAGHDREDALEHFRQSGATAFVKPLLGSRGDFAQVLHGEAALVRYLDEVAQYY
                     DSILIQPVVEGVEYRIFLLDDEVVYAARKHPPSVWGDGVATIRDLLTAHNQALRSRGL
                     SPVSVTDRDASLDGVLPDGERWDIPGRMNLSAGGTMVLEVPRSLAAFTLARKAARALG
                     LRVAAVDMFTDIGGDPDAMRIIEVNSNPSIRLLEDTGRGDLILKIWHHTFSAMGLIGV
                     "
     gene            1981..2754
                     /locus_tag="VGA15_01250"
     CDS             1981..2754
                     /locus_tag="VGA15_01250"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX9586357.1"
                     /translation="MSAFHREKVLSVRHWTDTLFSFTATRNSGFRFQNGQFAMIGLEV
                     DGRPLLRAYSMASANHEEALEFFSIKVADGPLTSRLQQIKEGDTILVGRKATGTLIAD
                     NLIPGKRLLLLSTGTGLAPFASLIKDPDVYDRFETIVLVHGCRQVSELAYGEELVAKL
                     RDDELIGPLLAEKLLYYPTVTREPFRNRGRITDLITSEQLFNDIGLPPLNIDTDRIMM
                     CGSPGMLEELRQTFEARDFLEGSHSEPGHFVIEKAFVER"
     gene            3289..>3741
                     /locus_tag="VGA15_01255"
     CDS             3289..>3741
                     /locus_tag="VGA15_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9586358.1"
                     /translation="MSVTDHADLTKTAFVFAGGGSFGAIQVGMMHSLAAHGISADMVV
                     GSSVGALNGAYYAGDPTLKGVQQLETIWRGLQRHDIFPITWRTLLGFVWRRDFLIPHD
                     GIQKLIDDHLPYRNLQDAKLPLHIVTTDIVSGDSVVLSEGSAAQAIIAS"
BASE COUNT          757 a         1120 c         1115 g          749 t
ORIGIN      
        1 gaccgtttcg ccgccaatcc cgatctcgtc gcggacgaac agcgatgcga tgccgcgggt
       61 gcgattatat tcggtgagat gcggccgcag gctgcggcag ttctcgccgt agacgatggt
      121 gccgcctgcc gcacaggcat cgctcttgtc ggaggcaatc aattccagcg agttgccgag
      181 cagttcgaca tgttcgtaat cggccgaggt cacgcaggtc gcgagcgcgc cgaccaggcg
      241 taccggatcg tagcggccgc cgatgccggc ctcgaacacc gcgaaatcgc attgccgctc
      301 ggaaaagtgc aggcaggcca gcgcgaacat ggcttcgaag gcgccgaacc gctgattgct
      361 ccgctgtgca atttcggtga tcgcggatcc gatccgcgcc acgagacgcg ccagatcatc
      421 atcgccgatt ggcacgccat cgacctgaaa ccgttcgttg aagcgataga gatgcggcga
      481 ggtgaacaat ccggttcgca agccgcaagc gtgcgcgata ccggcgcaga acgccgcggt
      541 agaacctttg ccgttggacc cggtcacgac gaccgaaatg cgcgagagcc tggcgcgatc
      601 gacctcgacg gcgtcgagca ggtccgccag ccgggcgaga caaacgccat cgccatattt
      661 cggaagatca aacacctatc agtcccatgg cagaaaatgt gtgatgccag attttcagga
      721 tcagatcgcc ccggccggta tcttccaaga gccggatcga cgggttggag ttcacttcaa
      781 tgatccgcat tgcatcggga tctccgccga tatcggtaaa catgtccacc gcggcgacgc
      841 ggaggccgag cgcccgggcg gcctttcgcg ccagggtgaa cgcggccagg gaacgcggaa
      901 cttcgagaac catcgtgcct cccgcgctca ggttcatccg cccgggaatg tcccaacgct
      961 cgccgtcggg cagcacgcca tcgagcgaag cgtcgcggtc cgtcacggaa accggcgaaa
     1021 gaccgcggga tcgcagggct tggttatgcg cggtcaggag atcgcggatg gtggctacgc
     1081 catcgcccca aacagatggc ggatgtttgc gcgccgcata cacgacttca tcatcaagca
     1141 gaaaaattct gtattcgacc ccttcgacca ccggctgtat caggatcgag tcgtaatatt
     1201 gcgcaacctc atcgagatat cggacaagcg cggcttcgcc gtgcaggact tgcgcgaagt
     1261 cgccgcgcga tccgagcagc ggcttgacga aggccgttgc gcccgattgt ctgaaatgtt
     1321 cgagcgcgtc ctcgcggtca tgacccgcgg cgcgacgggc gcggtgccgc tcatgcagga
     1381 agaaatattc gccgcccagc gtcgctgcgc cggcgcgttc aagaatggta ttggcgaagt
     1441 atttgtccga cgccagcgtc gaggctgtgg cgttgttttg cggataccac gaacatcgcc
     1501 cggcgccgaa atgaatggtt ttggtcttcg aggcaacgct gaacacaagg cccgttccac
     1561 cgtcgatgtc ttgaaatgcg agaccaaatt cagcgcaggc atattcggca tagatcgact
     1621 gatccggata ggatcccggc tttcccgatc gaaatttttc aaggccccgc atgcattatc
     1681 cggtggtcag ctagttcgaa ttgacagccc cgatctctta cgaaatttcc aagtgaaggc
     1741 ggcaattaac agccagttgg agaccgaatc ccgcaagtta attgcgcgta cacggcgtat
     1801 ttggcaaaat aaatcgactg ccattttgag cccgcaattg cgcctcgggg cttgttgact
     1861 atctctgaca atgcggtacg tcacacacca aatcgtcaac aattgctagg gttttcgcca
     1921 gatctcaggg cgagacaacg cgatcattct acatcaaggc aatcggaaaa accaaaacac
     1981 atgagcgcat tccatagaga gaaagttctt tctgtccggc actggaccga tacgcttttc
     2041 agcttcaccg ccacccgcaa ttccggcttt cgattccaga acgggcagtt cgcgatgatc
     2101 ggcctcgagg tcgatggccg tccgctgttg cgcgcctaca gcatggccag cgccaatcac
     2161 gaagaagcgc tggaattctt cagcatcaag gtcgccgacg ggccgctgac ctcaaggctg
     2221 cagcagatca aggagggcga taccatcctg gtcggacgca aggcgaccgg cacgctgatt
     2281 gccgacaatt tgatcccggg caagcgcctg ctgttgctgt cgaccggcac cggcctggcg
     2341 ccgttcgcga gcctgatcaa ggatccggac gtgtacgacc ggttcgaaac catcgttctg
     2401 gttcacggct gccggcaagt atccgaattg gcctatggcg aggagctggt ggcgaagctg
     2461 cgggacgatg aactgatcgg cccgctcttg gccgagaagc tgctgtatta tcccaccgtc
     2521 acgcgcgagc cgttccgcaa ccgcggccgg atcaccgatt tgatcacgtc cgaacaattg
     2581 ttcaacgaca tcggtctgcc gccgctgaat atcgacaccg accggatcat gatgtgcggc
     2641 agtccgggca tgctcgagga actgcgccag acctttgaag cacgcgattt tctcgagggc
     2701 agccacagcg agcccggcca ttttgtgatt gaaaaggcgt tcgtcgagcg ttgagccccg
     2761 cgtcccgggc gctatgtgcc cttggcgaag cgaagaaact cccggtcgcg tcgctccgct
     2821 gctggcggcg agcgccggtg cgcagggaga tggttggcag tctgtggata ccggctgtct
     2881 tagtcgcctg tttttgcgcg acgtgcaccc tattgaacat acaactattc aagaatctga
     2941 ttggcttgat gccatcgcgg cccgaccact cgggccaatc ccgtgacaga gagtgttttg
     3001 ctccccggca ccagatcgga ggggaccacc atgccccagt caacacagcg gtcaacacag
     3061 ttcgtcccga tcatccggca ttcttccccg atgccttgga ttcgtgccat cgccatcagt
     3121 ctcgtgcttt ggtcattgat catttgggga gttgttgtcg ccctgtcgtg atcttggacg
     3181 ggatcggcag atatgtcggt ggcgtggctt gcgcgtcggc tgctataaac ccggccgaac
     3241 gtcgcgtggt ctttcgccaa cgcgtcgagt gcgggagcga ctgaggtttt gtctgtaacc
     3301 gatcatgccg acctgacgaa aacggccttt gtattcgcgg gcggtggcag ctttggcgcc
     3361 attcaggtgg gcatgatgca ttcgctcgcc gcccacggaa tttcagcgga catggtggtc
     3421 ggctccagtg tcggcgccct gaacggcgcc tattacgcgg gcgatcccac gctcaagggc
     3481 gtgcagcagc tggaaaccat ttggcgcgga ttgcagcggc acgacatttt cccgatcacc
     3541 tggcggaccc tgctgggatt tgtctggcgc cgggattttt tgatcccgca tgacggtatc
     3601 cagaagctga ttgacgatca tctgccctat cgcaatctgc aggatgcaaa acttccgctt
     3661 cacatcgtca cgaccgatat cgtctctggc gacagcgtcg tgctgtcgga ggggtcggcc
     3721 gcccaggcga tcatcgcctc t
//
LOCUS       DASWGM010000036        10366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_3141, whole
            genome shotgun sequence.
ACCESSION   DASWGM010000036 DASWGM010000000
VERSION     DASWGM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3141"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..189
                     /locus_tag="VGA15_01260"
     CDS             25..189
                     /locus_tag="VGA15_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX9586359.1"
                     /translation="MSAILTVQNFTSDFVATLWTPIFVGIFIAIVTYALWPRNKDAFD
                     EASKMPLREE"
     gene            196..1068
                     /gene="ccoP"
                     /locus_tag="VGA15_01265"
     CDS             196..1068
                     /gene="ccoP"
                     /locus_tag="VGA15_01265"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEX9586360.1"
                     /translation="MTEHSDFDHVSGKSTTGHEWDGIKELNTPLPRWWVLAFYACIVW
                     AVGYWFVYPAWPLLWSNTTGILHYSSRADVALELANLEKIRGEKMVTLGAASLADIEK
                     DPALLALARARGKTVFGDNCAPCHGSGGAGAKGFPNLNDDDWLWGGTLDQIMRTIQFG
                     ARSGHAKTHESQMLAFGKDGTLKTEQIVTVANYVRSLSGLPTSQGYDATAGAKIFAEN
                     CAVCHGDAGKGNQELGAPDLTDKIWLYGSDEAAVIETITNGRAGVMPAWVDRLDPSTI
                     KAMAVYVHSLGGGK"
     gene            1172..2635
                     /gene="ccoG"
                     /locus_tag="VGA15_01270"
     CDS             1172..2635
                     /gene="ccoG"
                     /locus_tag="VGA15_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX9586361.1"
                     /translation="MTKSALPIELQWEDDGPLYVAQKKVYPQSVSGTFRRIKWGLMAF
                     CLSVYYLLPFVRWNRGLGAPDQAVLIDLPNSRFYFFFIELWPQEVYYFTGLLILAAIT
                     LFLMNSVGGRIWCGYLCPQTVWTDLFYAVERLVEGDRRERMKKDAARGTLKLQRFAEI
                     TLKHSIWLLIAWWTGGAWVLYFNDAPTLVKQLVTFQAPMLAYIWIAILTATTYVLAGF
                     MREQVCVYMCPWPRIQAALTDEWALNVTYKYDRGEKRTSLKKANELRASGETAGDCID
                     CYQCVAVCPTGIDIRDGSQLDCIQCGLCIDACDTVMKKIGREPRLIGYDNDINIHRRQ
                     AGKPEIFRVVRPRTIVYCAMIAGVGAIMLYALLTRSLLDVNVLHDRNPVAVKLSDGSV
                     RNAYTVRLLNKRGFDRVVAIDIDGPHNATLHVVGVDSVTPDRPMIVLGRDQTTELRLL
                     VTAPSENNSEKSIPVKFRVTDIGLGEAASATDNFVSP"
     gene            2655..3155
                     /locus_tag="VGA15_01275"
     CDS             2655..3155
                     /locus_tag="VGA15_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX9586362.1"
                     /translation="MNSALVSPKPLTGAKVLFMLVAFFGVVFGVNFLMMKLAIDTLPG
                     TEVDSAYSASMAYENEIGAAHDQNARNWKVDAHIQRGPDGGATLEVEARDNSGKPMSG
                     LKFQGRFERPTDRRADLPVALSETGIGIYRGSAPVIAAGQWDLVLEGDAAGRRMFLSK
                     NRVLLN"
     gene            3170..5362
                     /locus_tag="VGA15_01280"
     CDS             3170..5362
                     /locus_tag="VGA15_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX9586363.1"
                     /translation="MQATRDFSHYVRDAGAGLQHIDLAVEGVSCAGCMSKIERGLSAM
                     PDVTLARVNLTDRRVALEWKEGALDPSRFIDRLAELGYKAYPFETESAEAAEAEQSRF
                     LLRCLGVAAFATMNVMMLSIPVWSGNVSDMIPEQRDFFHWLSALIALPAAAYAGQPFF
                     RSALQALRTRNVNMDVPISIGVMLALGMSVVETVNHAEHTYFDAAIMLLTFLLVGRYL
                     DQSMRRRTRAVAGNLAALKAETATKFVGADEIAEVPVAAINPGDIVLLRPGERCAIDG
                     TVIEGRSEIDQSLITGETLYATAEHGAAVYAGSLNISGTLRVRVSAASEGTLLAEITR
                     LLDNALQARSRYVRLADRASRLYAPVVHATALLTMLGWVLMGATWHDAIVTAVAVLII
                     TCPCALGLAIPTVQTVASGAMFRAGVLLNSGDSIERLADVDHVIFDKTGTLTPPDLEV
                     MNASEIPEDMLRLAGQLALASHHPVAASVARAANAKSPLAGAVEEPGQGVRGHAGGGE
                     VRLGRPSFCGAEQLANDSLRLDPEASIVAFSFGDAKYVFSVRQGLRPDAKAMISALLK
                     RRITVEILSGDREPAVRAAARALGISEWRAGVTPADKIARIEELKSQGFRVMMVGDGL
                     NDAPSLAAAYVSMSPISAAHLSQATADLVFLGKPLAPVVAAIDFSRKALHLMRQNLWL
                     AVGYNLLAVPIAISGAVTPLIAAAAMSGSSLLVMLNALRARRVVHGDA"
     gene            5362..5526
                     /gene="ccoS"
                     /locus_tag="VGA15_01285"
     CDS             5362..5526
                     /gene="ccoS"
                     /locus_tag="VGA15_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX9586364.1"
                     /translation="MEVLVILVPLALGLGLVGLLGFLWSLKSGQYDDLEGAAWRAIQD
                     DEPARETPSD"
     gene            complement(5533..6747)
                     /locus_tag="VGA15_01290"
     CDS             complement(5533..6747)
                     /locus_tag="VGA15_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9586365.1"
                     /translation="MTDIFAEMKSETMPMHGHSPGVVLRSLIIGLTAFLTVVDLFATQ
                     AILPSLTRHYNVTPAAMGFAVNASTMGMAVAGLVVGFLSQHIDRRLGILLSLALLAIP
                     TSLLAVAPNLAVFTILRIMQGLCMASAFALTLAYLGEQCSSMDAGGAFAAYITGNVAS
                     NLIGRLVSAAVVDTLGLASNFYFFALLNLAGAVLVYFTIQRVQPMHSMAMTQTPFAAM
                     IGHWRNPALRAAFGIGFCILFAFIGTFTFVNFVLVRPPLSLGRMDLGFVYFVFLPSIV
                     TTLLAGKAVAAVGTRPAISGALAVAAIGLPLMLSSHLAEVLAGMVLVGAGTFFAQASA
                     TGFVGQAASESRGVASGTYLACYFLGGLVGSAVSGQLFDRFGWIACVAGVAASLAMAA
                     LLTARLTISGKG"
     gene            complement(6877..7368)
                     /locus_tag="VGA15_01295"
     CDS             complement(6877..7368)
                     /locus_tag="VGA15_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9586366.1"
                     /translation="MFKLTTNSRALWQSFALACVIAGSAATATTAFAGECPADKMKAN
                     AREKVDFKPVGVTDVTLGSIDLEKQPAHLKDRELRFRKLTIEPGGIVPWHSHDDRPAL
                     IFVQQGEIVEYASNCSEPIVHKAGDIRPEVFGTSHWWKNLGTQTVILYVGDVRKDPHD
                     HNM"
     gene            complement(7504..8865)
                     /locus_tag="VGA15_01300"
     CDS             complement(7504..8865)
                     /locus_tag="VGA15_01300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9586367.1"
                     /translation="MSIETFDVVILGGGNAGIGVTGPVRRAGMSVAMIESHDLGGTCP
                     NRGCTPKKVLVAAGHALHEIERAAVHHISVGKPKLDWAALIDREKDLIKDIPANLARS
                     MAHRHVEVIKAHAVFAGPNAIRIGQRHLEARHIVIATGSKPRPLPIPGAEHMITSDEM
                     LSERTLPGSVIFVGGGVISLEFGHVYARAGADVIILEALPQLLPAMDADAVARIQTES
                     ERIGIRIKTSVSVTRIEPANDRLRVIFTHEGTEHAAEADRVVNGAGRVANVDMLDLAS
                     GNIDHANGRVAVDRHLRSTSNPQVYVCGDAVPISPQLSPIATYEGDIVGRNIVEGPKY
                     SPDYGSMATSVYTVPALAAVGLTEAAASQKGLAIKVHTNDMHDWFSARTYAETVAWSK
                     IIVDQSTDRIVGAHFVGHAGEELVNIFGLAMRFGITAGQIRDNVYAYPTFSSDITHMI
                     GHS"
     gene            9271..9978
                     /locus_tag="VGA15_01305"
     CDS             9271..9978
                     /locus_tag="VGA15_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX9586368.1"
                     /translation="MTQLSPANAERLKLAFVQCRDMEGTLNERLGAYAVAGREIFPAY
                     GEAVDRLVARIHQNGGGENAPRPGDPMPPFILPDVTGRLVDLKSVLEKGPVAVMFYRG
                     HWCPYCRLNVGAVIKAMDRIKVLGGQVVAIMPEVQQFAEKFKADSDAPFPVLTDLDNG
                     YALSLNLAIWLGTEIQRLLSYQDMKTFHGNDGWMLPIPATFVVGRDGLVKARFVDPDF
                     RKRMEIDDLIAAFRAAS"
     gene            complement(10027..>10366)
                     /locus_tag="VGA15_01310"
     CDS             complement(10027..>10366)
                     /locus_tag="VGA15_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9586369.1"
                     /translation="EDLQTQQLLFRNFCEHAERLSASFRGHGIDVDHSHGISSERDLI
                     ELLEADIGIAVIPCSVSTPETLKRANVDGLDMRRTVHLYGVAGRERTAVASAVMRMLR
                     GADWSHYLGA"
BASE COUNT         1980 a         3047 c         3322 g         2017 t
ORIGIN      
        1 aaatcttcac ctggaagcag aacgatgagt gccatcctaa cagtccagaa tttcacatcg
       61 gatttcgttg cgacgctgtg gacgccgatc ttcgtcggaa tattcatcgc gatcgtgacc
      121 tacgcgcttt ggcctcgcaa caaggacgct ttcgacgagg cgtcgaaaat gccgttgcgg
      181 gaggaatgac agatcatgac cgagcacagc gatttcgacc acgtctccgg caagagcacg
      241 accggccacg agtgggacgg catcaaggag ttgaatacgc cgctgccgcg ctggtgggtt
      301 ctggccttct acgcgtgcat cgtctgggcg gtcggctact ggtttgtata tccggcgtgg
      361 ccgctgctgt ggagcaacac caccggcata ctgcattact cgtcccgtgc ggacgtcgcg
      421 ctagagctcg ccaatctgga aaagatacgc ggcgagaaaa tggtgacgct gggtgctgcc
      481 tcgcttgcgg atatcgagaa ggacccggcc ttgctcgctc tggcacgcgc ccgcggcaag
      541 acggtgtttg gcgataattg cgcgccgtgc cacggcagcg gcggcgccgg cgcaaagggc
      601 tttcccaacc tgaacgatga cgactggctc tggggcggca cgcttgacca gatcatgcgg
      661 acgatccagt tcggagcgcg ctcgggacat gcgaaaaccc acgagagtca aatgcttgcc
      721 ttcggcaagg acgggactct gaaaacggaa caaatcgtca cggtcgccaa ttacgttcga
      781 tcgttgtcgg gcttgcccac gagccagggg tatgatgcga ccgcgggcgc gaaaatcttc
      841 gccgaaaatt gcgcggtgtg tcatggcgac gcgggcaagg gaaatcagga gctcggcgcg
      901 cctgatctca ccgacaagat ctggctctat ggttccgatg aggctgccgt catcgaaacg
      961 atcaccaacg gccgcgccgg cgtcatgccg gcctgggtgg accggctcga tccctccacg
     1021 atcaaggcga tggcggtata tgtgcactcg ctcggtggag ggaagtgacc gcgggcgggg
     1081 cagggaaatc ctggatatcg tttgagctgg atcaactgcg cgttcgaagt tgagtctacg
     1141 ctcagccttg aacgcgagat ggcccccacc gatgaccaag tctgcacttc cgatcgagct
     1201 gcaatgggaa gacgacgggc cgctttatgt cgcccagaag aaagtctatc cgcagagcgt
     1261 ctccggaaca ttccgccgta tcaaatgggg cctgatggca ttctgcctca gcgtctatta
     1321 cctgttgcct ttcgtgcggt ggaaccgcgg tctgggcgcg cccgatcagg cggtgctgat
     1381 cgatctgccc aacagccgct tctatttctt tttcatcgag ctgtggccgc aggaggtcta
     1441 ctatttcacc gggctgctga tcctcgccgc cattacgctg tttttgatga attcggtggg
     1501 cggccgtatc tggtgcggat atctctgccc acagacggtg tggacggatc tgttctacgc
     1561 cgtcgagcgt ctggtcgagg gcgaccggcg cgagcgcatg aagaaagatg ccgcgcgtgg
     1621 cacgctgaag ctgcagcgtt ttgcggaaat taccctgaaa cattcgatct ggctgttgat
     1681 cgcctggtgg accggcggcg cctgggttct ctatttcaac gatgcgccga cgctggtgaa
     1741 gcagcttgtg acgttccagg cgccgatgtt ggcctatatc tggatcgcga tcttgacggc
     1801 gaccacctat gtgctcgccg gcttcatgcg cgagcaggtc tgcgtctata tgtgcccgtg
     1861 gccgcgcatc caggcggcgc tcaccgacga atgggcgctc aacgtcacct acaaatacga
     1921 ccgcggcgaa aagcgcacgt cgctgaagaa ggcgaacgag ttgcgggcat ccggcgaaac
     1981 cgccggcgac tgcatcgatt gctatcaatg cgttgccgtc tgcccgaccg ggatcgatat
     2041 ccgtgacggc tcgcaactcg actgcatcca gtgcggcctg tgcatcgatg cctgcgatac
     2101 cgtcatgaag aagatcggca gggaaccccg cctgatcggg tacgacaacg acatcaatat
     2161 tcaccgcagg caggccggca agccggaaat cttccgtgtc gtcaggccgc gcaccatcgt
     2221 ctactgcgcc atgatcgccg gcgttggcgc gatcatgctg tatgcgctgc tgacgcgaag
     2281 cctgctcgac gtcaacgtat tgcacgaccg caatccggtc gcggtgaaac tcagcgatgg
     2341 atctgttcgc aacgcctata cggttcgcct cttgaacaag cgaggcttcg accgcgtggt
     2401 ggcgatcgac atcgacggcc cgcacaatgc gacgctccat gttgtcgggg tcgattcggt
     2461 tacgccggac aggccgatga tcgtcctggg gcgggaccag accaccgaac tcaggcttct
     2521 ggtaaccgca ccctccgaga acaattccga gaagtcgatc ccggtgaaat tccgggtaac
     2581 cgatatcggg ttgggtgagg ctgcatcggc aaccgataat ttcgttagtc cgtaacgttg
     2641 aagaggggct tcgtatgaat agtgcgttgg tttcgccgaa gccgttgacc ggggccaagg
     2701 tccttttcat gctggtcgcg ttctttggcg tggtgtttgg cgtgaacttc ctcatgatga
     2761 agctcgcgat cgatacattg ccagggactg aagtggacag cgcctacagt gccagcatgg
     2821 cctacgagaa tgagatcggt gctgcccacg atcagaacgc ccgaaactgg aaggtcgatg
     2881 cgcatatcca gcgcggcccg gacggtggcg cgacgcttga ggtcgaggcg cgcgataata
     2941 gcggcaagcc gatgtccggc ctgaaattcc agggccgctt cgagcgtcct accgaccggc
     3001 gcgcggatct gccggttgcg ctttcggaaa ccggaattgg catctatcgc ggcagcgcgc
     3061 ccgtgatcgc ggcggggcaa tgggatctgg tcctcgaggg tgatgcggca ggacggcgga
     3121 tgttcctgtc gaagaatcgc gtgttgttga attgaaggaa ggccgcagca tgcaggcgac
     3181 gcgagacttt tcacattatg tgagggacgc aggcgcgggc ctgcagcata tcgatcttgc
     3241 cgtcgagggc gtcagttgcg ccgggtgcat gtcgaagata gagcgcgggc tttcggcgat
     3301 gcccgatgtg acgcttgcgc gggtcaatct gaccgaccgc cgcgtggcgc tggaatggaa
     3361 agagggcgcg ctcgacccca gccggttcat cgatcggctg gccgagctcg ggtacaaggc
     3421 ctatccattc gaaaccgaaa gtgcggaggc cgcggaagcc gaacagtcgc gcttcctgct
     3481 tcgctgcctc ggcgtcgcgg cattcgccac catgaatgtg atgatgcttt cgattccggt
     3541 gtggtccggc aacgtcagcg acatgattcc ggagcagcgc gatttctttc attggctgtc
     3601 ggcactgatc gcgctgccgg ccgcggcgta tgcgggccag ccgttcttcc ggtcggcgct
     3661 tcaggccttg cgcacgcgca acgtcaatat ggatgttccg atcagcatcg gcgtgatgct
     3721 tgcgcttgga atgtcggtgg tcgagaccgt gaaccacgcc gagcatacct atttcgacgc
     3781 ggcgatcatg ctgctgacat tcttgctggt cgggcgctat ctcgaccaga gcatgcggcg
     3841 gcggacccgc gcggtcgccg gaaacctcgc cgcgctgaaa gccgagaccg cgaccaaatt
     3901 cgtcggcgcc gacgaaattg cggaggtgcc ggtagcggcg attaatcctg gcgacatcgt
     3961 cctgctgcgt ccgggcgagc gttgcgcgat cgatggcacg gtgatcgagg ggcgatccga
     4021 aatcgaccaa agcctcatca ccggcgaaac cctgtatgcg acggcagagc atggcgcggc
     4081 tgtttatgcc ggttcgctca acatctccgg aacattgcgg gtccgggttt cggcggcgtc
     4141 ggagggcacg ctgctggcgg agatcacccg gctgctcgac aatgcgttgc aggctcgctc
     4201 gcgctacgtg cgcctcgccg accgcgcctc gcggctttat gcgccggtgg tgcatgccac
     4261 cgcgttgctg acgatgctcg gctgggttct gatgggcgcg acctggcacg atgccattgt
     4321 cacggcggtg gcggttctga tcatcacctg tccctgcgcg ctggggctgg ctatccccac
     4381 cgtgcagacg gttgcgtccg gtgcgatgtt cagggccggc gtgttgctca attccggcga
     4441 ttccatcgaa cgtctcgctg acgtcgacca cgtcatcttc gacaaaaccg gcaccctgac
     4501 gccacctgac ctggaagtca tgaacgcaag cgagatcccc gaagacatgc tccgtctcgc
     4561 cggccagctt gcgctggcga gccatcatcc ggtcgcagcc tcagtggcgc gggccgcgaa
     4621 cgcgaaatcg ccgctggccg gagctgtcga ggagccggga cagggggtgc gcggccatgc
     4681 cggcggaggt gaggtcagac ttggaagacc gtcgttctgc ggggcagagc aattggcaaa
     4741 tgacagcctg cgcctcgatc ccgaagcttc catcgttgcc ttcagcttcg gcgacgccaa
     4801 atacgtgttt tccgttcggc aggggttgcg tccggatgca aaggcgatga tttccgcgct
     4861 tttgaagcgc cgtatcacgg ttgaaattct ctccggcgat cgggagccgg cggtccgcgc
     4921 cgccgcgcgc gcgctcggga tcagcgagtg gcgggcgggc gtgaccccgg ccgacaagat
     4981 cgcccgcatc gaagagctga agagccaagg ttttagggtg atgatggtcg gcgacgggct
     5041 gaacgatgcg ccttcgctgg ccgcggccta cgtctcgatg tcgccgatct cagcggcgca
     5101 tctgagccag gcaacggccg atctggtgtt cctgggcaag ccgctggctc cagtggtcgc
     5161 tgccatcgac ttctcacgaa aggcgctgca cctgatgcgg caaaatctct ggctcgcggt
     5221 cggctacaac cttctcgcgg tgccaattgc gatcagcggg gccgtgacgc ccctgatcgc
     5281 ggcggcggcg atgtcgggtt cctcgctgct ggtgatgctg aacgcgcttc gcgcgcgccg
     5341 cgtcgtgcac ggagacgcgt gatggaagtg ctcgtcattc tggtgccgtt ggcccttggt
     5401 ctcgggctgg tcggcctgct cggatttctc tggtcgctca agagcggtca gtacgacgat
     5461 ctcgagggcg ccgcctggcg cgccatccag gatgacgagc cggcacggga gacgccatcg
     5521 gattaagcgc gcttaccctt tgccggatat cgtcaggcgt gcggtcagta acgcagccat
     5581 cgccagcgac gcggccacgc cggcgacgca ggcaatccag ccgaagcggt cgaacaattg
     5641 gcccgagacc gcgctgccga cgagaccgcc caggaaatag caggcgaggt aggttccgct
     5701 cgccacgccg cggctttcac tggcggcctg tccgacaaaa ccggtcgccg acgcttgtgc
     5761 aaagaacgta ccggcgccga ccagaaccat gccggccagg acttccgcaa gatgcgagga
     5821 cagcatcagc ggcaggccga tcgccgcgac cgcaagcgcg cccgagatcg ccggccgcgt
     5881 tccgacggct gcgacagcct tgccggcaag aagcgtcgtc acgatagaag gcaagaagac
     5941 aaaatagaca aatccgagat ccatacgtcc cagcgacagc ggcggacgca ccaggacaaa
     6001 attcacaaag gtgaatgtcc cgataaaggc gaacaggatg cagaagccga tgccgaaggc
     6061 ggcgcgcagc gccggattgc gccagtgtcc gatcattgcc gcgaaaggcg tttgcgtcat
     6121 cgccatcgaa tgcatcggct gcacccgctg gatggtgaag tacaccagca ccgcgccggc
     6181 gagattcaac aacgcgaaga aatagaaatt cgaggccagc ccaagggtgt cgacgacggc
     6241 tgcggaaacc agccggccga tcaaattgct ggcgacgtta ccggtgatgt aggccgcgaa
     6301 cgcgccgccg gcatccatcg agctgcattg ttcgccgaga taggccagcg ttaatgcgaa
     6361 cgccgatgcc atgcacaaac cctgcatgat acgaagaatc gtgaatacgg caagattggg
     6421 cgcgaccgcc agcaggcttg tcgggatggc gagcagggca agactcagca agatgccgag
     6481 ccgccgatcg atgtgctggc ttagaaatcc gacgacaagt ccggcgaccg ccatccccat
     6541 ggtgctggca ttgacggcaa atcccatcgc cgccggcgtc acattgtaat gcctggtcag
     6601 cgaaggaagg attgcctgcg tcgcaaacag atcgacgacc gtcaaaaacg ctgtcaggcc
     6661 gatgatcagg gaacgcagca ccacgcccgg cgaatggccg tgcattggca tcgtttcgga
     6721 cttcatctcc gcgaatatgt cggtcatggc gcggtgcttc ctgcgaaagc tgggttaagg
     6781 aaggggatgt gacgcccggg cgtgtggggc aacacggggg ctcccgggac gtcacatcag
     6841 ataacggggc ttgtaacgag gcttgcggcg cgctcgttac atattgtggt cgtgcggatc
     6901 cttgcggacg tcgccgacat acagaatgac cgtttgggtc ccgaggttct tccaccaatg
     6961 cgaggtgccg aacacctcgg gcctgatatc gccggccttg tgcacgatgg gctcggaaca
     7021 gttgctggca tattcgacga tctcgccctg ctgcacaaag atgagcgccg gccgatcgtc
     7081 gtggctgtgc cagggcacga tgccgcccgg ctcgatcgtc agcttgcgga aacgcagctc
     7141 acggtccttg agatgcgccg gctgcttttc gagatcgatc gaaccgagcg tgacgtcggt
     7201 gacgccgacc ggcttgaagt cgactttctc gcgagcgttg gctttcattt tatcggccgg
     7261 acattcgccg gcgaaagcgg tcgtcgccgt tgctgccgat cccgcgatga cgcaggcgag
     7321 tgcaaagctt tgccagagcg cgcgcgaatt tgtggtgagc ttgaacatgt cattatctcc
     7381 tctgttatgc cgtgatctcg aatgatctgc ccggctacag gacgaagctt cgttgaaatc
     7441 acgtgctcaa ggaaatgccg gttggctttt gaatcgatag cgcagcgcta tgtggagttg
     7501 tcttcaagag tggccgatca tgtgcgtgat atcggaggaa aacgtcgggt aggcatagac
     7561 gttgtccctg atctggccgg cggtgatgcc gaagcgcatc gccagcccga agatgttgac
     7621 aagttcctcg ccggcatgtc cgacgaaatg cgcgccgaca atacggtcgg ttgactggtc
     7681 gacgatgatt ttcgaccagg cgacggtctc cgcgtaggtc cgtgcggaga accagtcgtg
     7741 catgtcattg gtatgaacct tgatcgcaag gcccttttga cttgccgcgg cttcggtcag
     7801 acccaccgcg gcgagcgcgg ggacggtgta gacggaggtc gccatgctgc cgtagtccgg
     7861 actgtatttc ggtccctcga cgatgttgcg gccgacgata tcgccctcgt aagtcgcgat
     7921 cggcgagagc tgcggagaaa taggcaccgc gtcgccgcag acatacacct gaggattgga
     7981 agtcgagcgc agatgacggt ccaccgccac gcggccgttg gcgtggtcga tatttcctga
     8041 cgccagatcc agcatatcga cattggccac gcgtccggcg ccattgacga cacgatcggc
     8101 ctcggcggca tgttcggtgc cttcatgcgt aaagatcacg cgcaggcgat cgttcgcggg
     8161 ctcgatccgt gtcacgctga cgctggtctt gatgcgaatt ccgatgcgct cgctctcggt
     8221 ttggatacgc gcgacggcgt cggcatccat cgcgggcaac aactgcggca acgcttcgag
     8281 gatgatgacg tcggcgccgg cgcgcgcata aacgtggcca aattccagcg atatgacgcc
     8341 gccgccgacg aagatcaccg aacccggcaa ggtccgctcg ctcagcattt cgtccgacgt
     8401 gatcatatgc tcggcgccgg gaataggcag tgggcgtggc ttcgatccgg tcgcgatcac
     8461 gatgtgcctg gcctcgaggt gacgctggcc aatgcggatc gcattgggcc cagcgaaaac
     8521 cgcgtgggcc ttgatcacct cgacatggcg atgcgccatc gagcgggcga gattcgcagg
     8581 gatatccttg atcaggtctt tctcgcgatc gatcaacgcg gcccagtcga gcttcggctt
     8641 gccgaccgag atgtggtgaa cggcggcgcg ctcgatttcg tgcaaggcat gaccggccgc
     8701 gaccagcacc ttcttcggcg tgcagccacg gttcggacag gttccgccga ggtcgtgcga
     8761 ttcgatcatc gcgaccgaca ttccggcgcg tctgacaggg ccggtgacgc caatgccggc
     8821 gttgccgccg cccaggatca ccacgtcgaa tgtctctata ctcatcgaag atttccctat
     8881 gtcgcagttg atccctggga cgccgcgcgg tcaggtcgcg tcattggcgc ggcctgaacc
     8941 cattacgcgc ggtgtgcggg cgttgttact ggtcagtgac ttggtcgtga tcgtcaggat
     9001 gaacgcgcca ttcagtaata tgaaatacgc actcgctttg aagtcgatag ctgcgcgcta
     9061 ttagggcagg gcgatgctcc ccggctatca aaccgattgt gcaacggcat ttctaacatc
     9121 aggccattca gcctctaata agtgagcccc ccttgagggc ggcgtgtgca ggaaaatggt
     9181 acaatgtccg agaaatccaa ttacggtgcc gtggggtgcc gccatatgtt tcagtctgaa
     9241 tctggaaagc ccaatccgcc ggagcaagac atgacacagc tttcacctgc caatgccgag
     9301 cgtctcaagc tggccttcgt gcaatgccgc gacatggagg ggacgctgaa cgaacggctg
     9361 ggcgcctatg ccgtggcggg gcgggagata tttccggcct atggtgaggc ggttgatcgc
     9421 ctggtggcgc ggatccacca aaacggcggc ggagaaaacg cgccgcggcc gggtgatccg
     9481 atgccgccct ttatccttcc ggatgtgacc gggcggctgg tcgatctgaa atccgttctg
     9541 gaaaagggac cggtcgccgt gatgttctat cggggtcatt ggtgccccta ttgcaggctg
     9601 aacgtcgggg cggtgataaa agctatggac cggatcaagg tgttgggcgg gcaggttgtc
     9661 gcgatcatgc ccgaggtgca gcaattcgct gaaaagttca aagccgattc agacgcgccg
     9721 ttcccggttc tcaccgatct cgacaatggc tatgcgcttt cattgaatct cgcaatttgg
     9781 ctcggaaccg aaatacagcg gctgctgtcg tatcaggaca tgaaaacgtt tcacggcaat
     9841 gacggctgga tgcttccgat tccggcgacg ttcgtggtcg gccgcgacgg gctggtgaag
     9901 gcgcggttcg tggatcccga cttccgcaag cgcatggaaa tcgacgacct gatcgccgcg
     9961 ttcagagccg caagctagat cctttggagc tgccggttga tcgccggcgc cgccggcgtc
    10021 gcctgttcac gcgccgagat agtgcgacca gtcggcgcca cgcaacatcc tcatcaccgc
    10081 tgatgctacg gcggtacgct cgcgcccggc aacgccatag aggtggacgg tacggcgcat
    10141 gtcgagtcca tcgacatttg cccgtttgag ggtttccggc gtcgatacgc tgcacggaat
    10201 cacggcgatg ccgatatcgg cttccagcag ttcgatcagg tcgcgttccg atgaaatgcc
    10261 atggctgtga tcgacgtcga tgccgtggcc gcgaaacgat gcgctcagcc gctccgcatg
    10321 ttcgcagaaa tttcgaaaga gcaattgctg cgtttgcaga tcctcg
//
LOCUS       DASWGM010000037         8978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1387618,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000037 DASWGM010000000
VERSION     DASWGM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387618"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1485
                     /locus_tag="VGA15_01315"
     CDS             199..1485
                     /locus_tag="VGA15_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9586370.1"
                     /translation="MNLRLYLCAIAAALSLDVEAAWAQLSGHGGPVRALAISADGKSV
                     LSGSFDTSAIRWSLATDSAEQVLRFHSDAVNAVVFLQDGRMATAGADARIAIWTAGRQ
                     QPDEIFEGHRAPISGLAVSPDGSMLASASWDRTVRLWPLGSGATRVLEGHLQNVNGVA
                     FMPDGKSLVSVGYDLAVRIWPLPDGPPDVITLTSPLNAVAIAPDGEIAAGGADGKVRF
                     LTVAAKEAGEVQAGPTPIVALAMSRDGTLIAAAGIGGAVALIDRKARSVLRTLGGPGL
                     PVWSVAFLPDNATVLTGGADGAIRRWNALTGDPIGSSLRGTPADPLAAFAGDRGAEIF
                     RACVACHTLSEKDNARAGPTLAGLFGRRIASLPGYRFSDALKSMNIVWTPETVAKLFE
                     VGPNAYTPGTKMPEQHIGSPEDRKALTDFLARATAK"
     gene            complement(1486..3456)
                     /locus_tag="VGA15_01320"
     CDS             complement(1486..3456)
                     /locus_tag="VGA15_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9586371.1"
                     /translation="MRLDTAAAKRGCRNSEIATFRQLCRAEIDQFRNAAKAEGSLTVG
                     CTQEAPLFSDEAGERPERIDFVNLRETAGWSTEGAQAGPKMAALLAAAGEPAPEVPLV
                     NMSSEGVILIYGRDETAIEAGKLLADHLDVTVMIAGPAQITPPAVTSFPIVKGTIRNA
                     KGHFGAFELAIDDYAAPRPSSRDALIFEAPRNGATSRCDLVLDLSGGLPLFAAHDLRD
                     GYLRADPRDPSAMLRAVLKARDLVGSFDKPKYINFTAELCAHSRSKITGCHRCLDLCP
                     TGAITPDGDHVNINAEVCAGCGQCAAACPTGAASYAVPPADTLLHKLRAMLLIYDQAG
                     GTKPVVLFHDGPHGTPLIDALARHGTGLPANVLPLAVNEVTQIGLEAIVAAFAYGAAS
                     VRFLLRAKPLHDIAGLSQTIAMAEAILSGLGFSGRRVATIETDDPDSVGEALCGLEPV
                     ATVKPPATFRTVGKRRDLLRFALRELHRLAPAPIDVILLPEGAPLGAISVNVDGCTLC
                     LSCVSVCPTGALIDDPERPALKFVEDACVQCGLCVSTCPEKVITLKPQIDFTAATART
                     QIIKEEEPALCIRCNKPFGVKSTIDKIAAKLEGRHWMFPANDKRIDAIRMCADCRVIT
                     MSEQQFDPFVGVPERAAPRTTDDYLRERESKG"
     gene            3607..4374
                     /locus_tag="VGA15_01325"
     CDS             3607..4374
                     /locus_tag="VGA15_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate--protein ligase family protein"
                     /protein_id="HEX9586372.1"
                     /translation="MEDHLSPAARTRAFISEPIDLPPPFTLVRLRESGDAFTHALGIA
                     EQSGAGTLVYVGRFDLAEFAVVLEPDEPLRTARRAFYAGMAALTDALRAYAPPNKSVT
                     IDWPDAIRIDGGLVGGGRLGWPPSVKEDEPPPWLVFGAMIRTVSVTDAELGIHPLSSA
                     LEQEGFGDAGAVQVTESFARHLMLAIDAWQVDGFGGVAREYLTRLSRERQVSRRIDEN
                     GDLLTHRIGIDKTERRDLLKALAAPSWLDPKLGGPRL"
     gene            4371..4910
                     /locus_tag="VGA15_01330"
     CDS             4371..4910
                     /locus_tag="VGA15_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HEX9586373.1"
                     /translation="MKLLRTIALDPSDTFVFDVAAEPGDWAVSGAFRFCDRDPARLTG
                     KDRSAFRSGFLGVRAWGWSTLVQIVGATAADRRALVELLAAQLVERFGAPDLATARLA
                     AEEEVAFAESLCTHPTGSLIAVHRTATDGEVRESFRRLRLREGQGHGKAFSFMEVEDD
                     PEPDSDLSLVDMSLEPRRR"
     gene            4907..5914
                     /locus_tag="VGA15_01335"
     CDS             4907..5914
                     /locus_tag="VGA15_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEX9586374.1"
                     /translation="MKDFWISCGHHLLDRNASGGLVVTVEFLKAYFARPELMPPEDAC
                     AIERRLHRELLAEPRRPVGADEIAAIGDADARENWQFVLAFRDLLLRHPTLEAAYLAL
                     VRSGLINLPPLFVNQLIHVILRNALDDCDDPFVLRAAELFFRPQRIMLHDQSLLLGDE
                     EVIGGLKITPVLSLMSMLGATTDAQIDVLNEENADSYWQRSDQFDMGLDLTSGKPGPA
                     ALAEAMTCWISHLLGINVSIEPLTELRDAKLTWYVGLDSEATKIGDRLWHGEALDDRT
                     AGRVLALFRLSVTDAGLVAGIVEQEPVYLIMAMTPDLNLRMKPQNLLTGLPIKHLEAA
                     S"
     gene            5911..6429
                     /locus_tag="VGA15_01340"
     CDS             5911..6429
                     /locus_tag="VGA15_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEX9586375.1"
                     /translation="MSPAAVPLLRIPVGVVVERRKTDSPWIDFVWRGIGVLPDEPEMK
                     PWTVLREQEEATLYYAGSAMVDLYRSETARYRENLTTGAPNIWIVMSPSEDAWPYAVA
                     AVTADPAEGEAFTEAGANLVEAVPMPEALRDAIEKFVTEHHVEREFVKRERRRADPEA
                     LARRHHDEGGHE"
     gene            6426..7088
                     /locus_tag="VGA15_01345"
     CDS             6426..7088
                     /locus_tag="VGA15_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEX9586376.1"
                     /translation="MSDEEFLARWSRRKREAKVAADAPAPAQTAETPNPASPVTAEDP
                     ANAELDLSSLPPIDSITAATDVTAFLRQGIPQDLSRAALRRAWAADPAIRDFVGLAEN
                     AWDFNDPHAMPGFGPLDCSEEQLGALVDRIVGGVRGAADSPTTAIPEQKDASPSADSN
                     HEPLLESGAPETVADLSPAEEISSDELPSVPAAVQPEAPQSVESDQTSPRRRTHGSAL
                     PR"
     gene            7220..7999
                     /locus_tag="VGA15_01350"
     CDS             7220..7999
                     /locus_tag="VGA15_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX9586377.1"
                     /translation="MRDAGLDRAIDAAGGIAQLARKIGIAQPSVSNWNRVPAQRVIAV
                     EAATGVSRKQLRPDLYSEPAVEDDALDPVDAARVREYALLAALLSCPPSNALLGEIAR
                     LQGDVTPLGRAHAALAEAAAEVTTAGVEREYFDLFVGLGRGELLPYGSYYLTGFLNER
                     PLSRLRDDLAALGIERIENNFEPEDHAATLCEIMAGLAGGCFRASEEAQRAFFEKHVA
                     PWMGRLFADMEKAASAKFYGSVGSLGRLFLEIEAEAFTFAN"
     gene            8029..8238
                     /locus_tag="VGA15_01355"
     CDS             8029..8238
                     /locus_tag="VGA15_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX9586378.1"
                     /translation="MKDEKKVTVGRRDFLRKVSVGAVGAGATLAAPLVAPAQADSETN
                     DEKRKARYKETDHVKAYYRVNRYPT"
     gene            8250..>8978
                     /locus_tag="VGA15_01360"
     CDS             8250..>8978
                     /locus_tag="VGA15_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9586379.1"
                     /translation="MLIKRTQRQRSEFASRGSITDALAGQAGGLDRRTFLRRSGLAGG
                     GLAALSTLPIGSVRKAEAAAAGPLTSGATIRKSVCTHCAVGCTVTAEVLNGVWIGQEP
                     SWDSPINRGSHCAKGASVRELVHSDRRLRYPMKLVNGQWTRVSWDTAVNEIGDKLLQV
                     REKSGSDSVYWLGSAKMTNEGAYLFRKFGAFWGTNNTDHQARICHSTTVTGVANTWGY
                     GAMTNSFNDIRNAKTQIIMGGNPAE"
BASE COUNT         1604 a         2760 c         2943 g         1671 t
ORIGIN      
        1 gtctcccgcc gagagtcctc aaggacgaag cccgcccagt atgcaagccg cacctcaagg
       61 ctcaagcaag accgtcgtga agcctcacta atagtccgat gcatctggtt tgacctcgcg
      121 ccacgatgtc ctctaccgtc tggatggatt gaggcgcgat cgcgcttcgc gtgccggcac
      181 gacggcgatg gataagcgat gaacttgcgc ctgtatctct gcgcaatcgc tgcggcgctg
      241 tcgctggatg tcgaagctgc ctgggctcag ctctccggcc atggtggacc ggttcgagcg
      301 ctggcgattt cagccgacgg gaagagcgtc ctttccggta gtttcgacac ttccgccatc
      361 cgatggtcgc ttgcgaccga ctccgcggag caggttcttc gctttcattc tgacgcagtg
      421 aatgcggtcg tcttcctgca ggacggccgc atggcaaccg ctggagccga tgcgcggata
      481 gccatctgga cggcgggccg gcagcagccg gacgagatct tcgagggcca tcgcgcaccg
      541 atttcgggac ttgccgtctc gcccgacggt tcgatgctgg catccgcatc ctgggatcgc
      601 accgtgcggc tttggccgct tggttccggt gcgacgcgcg tgctggaggg ccacttgcag
      661 aacgtcaacg gcgttgcctt catgccggac ggaaaatcgc tggtcagcgt cggttacgac
      721 cttgcggtga ggatctggcc gttgccggac ggaccgccgg atgtcatcac gctgacatcg
      781 ccgctcaacg cggtggcgat cgcgcctgac ggagaaatcg cagcgggcgg tgccgatggg
      841 aaagttcgct ttctgaccgt ggcggcgaag gaggccggcg aggttcaggc cggtccgacg
      901 ccgattgtcg cgctggcgat gtctcgagac gggacgttga tcgcggcggc cggcataggc
      961 ggcgccgtag cgctcatcga tcgcaaggcg cgaagcgtgc ttcgaacgct cggcggtccg
     1021 ggtttgccgg tgtggtccgt ggcattcttg cccgataacg cgaccgtgct gacgggcggt
     1081 gcagacggtg cgatccgacg ctggaatgca ctgacgggtg acccgatcgg atcctcgctg
     1141 agaggcacgc cagccgatcc gctggccgcc tttgcagggg accgcggcgc ggaaatattc
     1201 agggcctgtg ttgcctgcca tacgctgtca gagaaggaca atgcgcgcgc aggcccgacc
     1261 cttgctggat tgttcggccg caggatcgcg tcactgcccg gctatcgctt ttccgatgcg
     1321 ctgaaaagca tgaacatcgt atggactccg gagaccgtcg caaaattgtt cgaggtcggt
     1381 ccgaacgcct atacgcccgg caccaagatg ccggagcagc acatcgggtc gccggaagac
     1441 cgcaaggcgc tcaccgattt cctggcgcgc gcaaccgcga aatagtcatc ccttgctctc
     1501 ccgctcgcgc aaatagtcgt cggtcgtgcg cggagccgcg cgttctggaa cgccgacgaa
     1561 gggatcgaac tgctgttcgc tcatggtgat gacgcggcag tcggcgcaca tcctgatggc
     1621 gtcgatccgc ttgtcgtttg ccggaaacat ccagtgccgg ccctcgagct tcgccgcgat
     1681 cttgtcgatc gtgcttttga cgccgaacgg cttgttgcag cggatgcaaa gcgcaggctc
     1741 ttcttccttg atgatctgcg tgcgtgccgt agcggcggtg aagtcgatct gcggcttcag
     1801 cgtgatgacc ttttccgggc aggtgctcac gcacagcccg cactgcacgc aggcgtcttc
     1861 gacgaacttg agtgccggac gttcgggatc gtcgataagc gcccccgtgg gacacaccga
     1921 tacgcaggac aagcatagcg tgcatccgtc cacattgacg ctgatcgcgc caagcggcgc
     1981 gccctcgggc agcagaatca catcgatcgg cgccggtgcc aggcggtgca gttccctcaa
     2041 ggcgaaacga agcaggtcgc gccgtttgcc gacggttctg aaagtcgcag gcggtttgac
     2101 ggttgcgacg ggttcaagtc cgcacagcgc ctcgccgacc gaatccggat cgtccgtttc
     2161 gatcgtggca acacggcgcc ccgaaaatcc taggccggaa agaatggctt cggccattgc
     2221 gatcgtctgc gaaaggccgg caatgtcgtg caagggcttg gcgcgcagaa ggaagcgtac
     2281 gctggccgcg ccgtaggcga atgccgcgac gatcgcttcc aacccgatct gcgtcacctc
     2341 gttgacggca agcggcaaca cgttggccgg caggccggtg ccatggcggg cgagcgcatc
     2401 gatcagcggc gtgccgtgcg gaccgtcatg gaacaacacg accggcttgg tgccgccggc
     2461 ctgatcatag atgagcagca tggcacgaag cttgtgcaac agagtgtcgg caggtggcac
     2521 agcgtaggag gccgcaccgg tcggacacgc cgcggcgcac tgaccgcatc ctgcacacac
     2581 ttccgcattg atgttgacgt gatcgccatc cggcgtaatc gccccggtcg ggcaaagatc
     2641 gagacagcgg tggcagccgg tgatctttga acgcgaatgg gcgcagagct ccgcggtgaa
     2701 attgatgtat ttcggcttgt cgaagcttcc gacgagatcg cgtgccttga gcacggcccg
     2761 tagcatcgct gacggatcgc gagggtccgc acgcagatag ccgtcgcgca ggtcatgcgc
     2821 ggcaaagagc ggcaggccgc cggaaagatc gagcacgaga tcgcagcgcg aggtcgcgcc
     2881 attgcgaggc gcttcaaaga tcaaggcgtc gcgcgatgag gggcggggcg ctgcgtagtc
     2941 gtcgatcgca agttcgaaag ctccgaaatg gcccttggcg ttgcggatgg ttcccttgac
     3001 gatcgggaac gatgtgacgg ccggcggcgt gatctgcgcc ggcccagcga tcatgacggt
     3061 gacatcgagg tgatcggcga gcagtttgcc ggcttcgatc gccgtctcgt cgcggccata
     3121 gatcagaatc acaccttcgc tcgacatgtt gacgagcgga acttcgggcg cgggctcgcc
     3181 cgcggcggca agcagtgcgg ccatcttggg tcctgcctgg gccccttccg tggaccagcc
     3241 cgcggtctcg cgcaggttga cgaaatcgat ccgctccggg cgctcgcctg cctcgtccga
     3301 gaacagcggc gcctcctggg tgcagccgac ggtgagtgat ccctccgcct tcgcggcgtt
     3361 gcggaactga tcgatctcgg cccggcagag ttgacggaaa gttgcgatct cgctgttgcg
     3421 gcatccgcgc ttagcggcgg ccgtatcgag gcgcatcgtg tcttcgcacg agcacatcag
     3481 gatcgttttt ggtggctgcg ccatgcccgg ctaaacactc cctcaaagcg tgcggagcgg
     3541 cagcgaagcg gactcgcgcc gacggtccgc ctcgtatagt tatgctcaat aggaaagaaa
     3601 aggaaggtgg aggaccacct gtcgccggcc gcacgcactc gcgcgttcat atcggaaccc
     3661 atcgacctgc cgccgccgtt taccctggtg aggctgcgcg aaagcggtga cgcgtttacc
     3721 cacgcgctgg gtatcgccga acaaagcggc gccggtactt tggtctatgt tgggcggttt
     3781 gacctcgcag agttcgccgt tgtgctcgag ccagacgagc cattgcgcac cgcgcgccgt
     3841 gccttctacg cggggatggc ggcgctgact gacgccttgc gcgcctatgc gccgccgaac
     3901 aagtcggtga caatcgattg gcccgacgcg atccgcatcg acggcggtct ggtcggcggc
     3961 gggcggctcg gctggcctcc atccgtcaaa gaagacgagc cgccgccatg gctggtattc
     4021 ggtgctatga tccggaccgt ctcggtgacc gacgcggaac tgggcattca tccgctctcc
     4081 tcggcactcg agcaggaggg ttttggcgat gcgggcgccg ttcaggtgac ggaaagcttt
     4141 gcgcggcatc tgatgctggc catcgacgct tggcaggtcg acggatttgg cggcgtggcg
     4201 cgcgaatact taactcggct ctcgcgtgaa cggcaagtca gccgccggat cgacgagaac
     4261 ggcgatctcc tcacgcaccg gatcggcatt gacaaaaccg agcggcgcga tctgctcaag
     4321 gccttggccg cgccgtcgtg gctcgatccg aaactcggag gaccgcgcct gtgaagctct
     4381 tgcgcaccat cgcccttgat ccatccgaca cgttcgtgtt cgacgtggcg gcggaacccg
     4441 gcgactgggc cgtgtcgggc gcgttccgtt tctgcgaccg ggatcccgcc aggctgacgg
     4501 gaaaggaccg ttcggccttc cgcagcggct ttctcggcgt gcgagcctgg ggctggtcga
     4561 cgctggtcca gatcgtggga gcgacggcgg ccgatcggcg tgccctggtt gaacttcttg
     4621 ccgcgcagtt ggtcgagcgg ttcggtgcgc cggatctcgc aaccgcgaga cttgcggcag
     4681 aagaagaagt cgcctttgcg gagtcgcttt gcacgcatcc cacgggcagc ctgattgcgg
     4741 tccaccgcac ggcgaccgat ggtgaagttc gggagtcatt tcgcagattg cggctgcgag
     4801 aagggcaagg tcatggcaag gcgttctcat tcatggaggt cgaggatgat cctgagcccg
     4861 acagtgatct cagtcttgtg gatatgagct tggagccgcg gcgccgatga aggatttctg
     4921 gatttcctgc ggccaccatc tgcttgaccg caacgcgagc ggcgggttgg tggttacggt
     4981 tgagttcctg aaagcctatt ttgcgcgtcc cgagctgatg ccgcccgaag acgcctgcgc
     5041 gatcgaacgc aggttgcacc gggaattgct cgccgagccg cgccggccgg tcggtgcgga
     5101 cgagattgcc gcaatcggcg acgccgatgc ccgggagaat tggcaattcg tgctggcgtt
     5161 ccgcgatctg ttgctgcggc atccgacgct cgaagccgcc tatctcgcgt tggtgcgctc
     5221 cggactcatc aacctgccgc cgctgttcgt caaccaactc attcatgtca tcttgcgaaa
     5281 cgcgctcgac gattgcgacg atccgttcgt cctgcgcgcg gccgagctgt ttttccggcc
     5341 gcagaggatc atgctgcacg accagtcgtt gcttttgggg gacgaggagg tcattggcgg
     5401 gctcaaaatc acgccggtat tgtccctgat gtcgatgctg ggtgcgacca ccgatgccca
     5461 gatcgacgtc ctcaatgagg aaaacgccga cagctactgg caacgcagcg accagttcga
     5521 catggggctt gatctcacca gtggaaagcc gggaccggcg gcgcttgcgg aggcgatgac
     5581 gtgctggatt tctcacctgc tcgggatcaa tgtcagcatc gagccgctca cggaattgcg
     5641 cgatgcaaag ctcacttggt atgtcgggct tgattccgag gccaccaaga tcggtgaccg
     5701 gctctggcat ggtgaggccc tcgacgaccg gaccgcgggc cgcgtgctgg cgctgttccg
     5761 cctcagcgtt accgacgctg gattggtggc cggaattgtc gagcaagaac cggtgtatct
     5821 gatcatggcc atgacaccgg acctgaatct ccggatgaag ccgcagaatc tgttgacggg
     5881 attgccgatc aagcacctgg aggctgcaag ttgagtcccg ccgctgtgcc cttgctgcgc
     5941 attcccgtcg gcgtcgtggt cgaacgccgc aagacggact cgccttggat cgatttcgtc
     6001 tggcgcggca tcggcgtctt gcccgatgag ccggagatga agccatggac ggttctgcgc
     6061 gagcaggagg aggcgaccct ttactatgcc ggtagtgcca tggtcgatct ctatcgctcc
     6121 gagacggcgc gttatcgtga gaacctcacg accggcgccc ccaacatctg gatcgtgatg
     6181 agcccttcgg aggacgcctg gccttacgcc gtggcggccg tgactgctga tcccgcggag
     6241 ggggaagcct tcacggaagc aggcgccaat ctcgtcgaag cggtgccgat gcctgaagct
     6301 cttcgcgacg cgattgaaaa attcgttacg gagcaccacg tcgaaaggga gttcgtcaag
     6361 cgcgagcggc gccgcgccga tcccgaggcg ctggcgcggc gtcatcatga tgaaggtggg
     6421 cacgaatgag cgacgaggaa tttcttgccc gttggtctcg tcgcaagcgc gaggcgaagg
     6481 tagccgctga cgctccggcg ccggcccaga cggctgagac gccgaacccg gcgtcgcccg
     6541 tcacggccga agatcctgcg aacgcagagt tggatctctc gagcttgccg ccaatcgatt
     6601 cgattaccgc ggctaccgac gtaacggcgt tcctgcgcca aggcatccct caggacttga
     6661 gccgtgcggc gcttcgtcgc gcctgggccg ccgaccctgc gatccgggat ttcgtcggtc
     6721 tcgcggagaa cgcatgggac ttcaacgacc cgcacgccat gcccggcttc gggccactcg
     6781 attgttcaga agaacagctt ggcgcgctgg ttgacaggat cgtcgggggt gtgcgcggcg
     6841 ccgctgacag ccctacgacc gcaatccctg agcaaaagga cgccagccca tcggctgatt
     6901 caaatcatga gccgttgttg gagtccggtg caccagaaac ggtcgccgac ctcagtcccg
     6961 ccgaggagat ttcgtcggac gaactgccct cagttcctgc tgcagtgcaa ccggaagctc
     7021 ctcagagcgt tgagagcgat caaacctcac ctcggcgtcg cacacacgga agcgcattgc
     7081 cccgctagac tttcgaccaa aggctatagc ctcaggctat gtcgattcaa ttgaccggcc
     7141 ccctgccgaa gtctagtctt cgctgaccaa aaaataaaaa ataatcaagc gcttcggaca
     7201 ccttggatgg gaggtccacg tgcgcgatgc gggccttgat cgtgccatcg atgcagcggg
     7261 cggcatagcc cagcttgccc ggaagatcgg cattgcccag ccttcggttt cgaattggaa
     7321 cagggtgcct gcccagcggg taatcgccgt cgaagccgcg accggcgtgt cgcgcaagca
     7381 acttcgcccc gatctctaca gcgagccagc tgtggaggat gatgctctcg atcccgtcga
     7441 tgctgcccgc gtgcgggaat atgcattgct tgccgccttg ctctcgtgcc cgccgtcgaa
     7501 cgcattgctt ggcgagatcg cgcggttgca aggcgacgta accccactcg gtcgcgccca
     7561 tgccgcgctg gccgaggccg cggcggaggt gacgaccgcg ggtgtcgagc gcgaatattt
     7621 cgatctgttc gtcggtctgg ggcgcggcga gctattgccc tatgggtcct actatctgac
     7681 cggctttctc aacgagcgtc cactatcgcg cctgcgcgat gatctcgctg cgctcggcat
     7741 cgagcggatc gagaacaatt tcgagcctga ggatcatgcc gcgacgctgt gcgagatcat
     7801 ggctggcctc gcgggcggct gcttccgagc gtcagaagaa gcgcagcgcg ccttcttcga
     7861 gaagcatgtg gcgccctgga tgggccggct gttcgccgac atggagaagg cagcgagcgc
     7921 caaattctac ggatcggtcg gctcgctcgg ccgcctgttt ctggaaatcg aggctgaagc
     7981 ctttacgttc gccaactgac caacggctgt tggtccggga gagatgcgat gaaggacgaa
     8041 aagaaagtga cagtcggtcg ccgcgacttc cttcgcaagg tcagcgtcgg cgcggtcggc
     8101 gccggcgcga cccttgccgc gccgctggtt gccccggcgc aggccgacag cgagaccaac
     8161 gatgagaagc gtaaggcgcg ctacaaggaa accgatcacg tgaaggcgta ttaccgcgtc
     8221 aatcgttatc ctacttgagg gaggcggccg tgctgatcaa gagaacacaa cgccagcgct
     8281 ccgaatttgc atcccgcggt tccatcacgg acgccttggc cggacaggcc ggcggtctcg
     8341 atcgccgcac tttcctgcgc cgatccggcc ttgccggcgg cggtttggcg gcgttgagca
     8401 cgctgccgat aggcagcgtc cgcaaggcgg aggctgcggc cgcggggccg ctcacctcag
     8461 gtgcaaccat tcgcaagagc gtctgcacgc attgcgccgt cggatgcacg gtgaccgcgg
     8521 aagtgctcaa cggggtctgg atcgggcagg agccgagctg ggattctccg atcaatcgcg
     8581 gatcgcattg cgccaagggt gcttcggtgc gcgaactcgt gcacagtgat cggcgacttc
     8641 gctatccaat gaagctcgtc aacgggcaat ggacgcgcgt ctcctgggac accgcggtca
     8701 acgagatcgg cgacaagctg ctccaggttc gcgagaaatc aggcagcgat tccgtctatt
     8761 ggctcggatc ggccaagatg acgaacgaag gcgcctatct gttccgcaaa ttcggcgcgt
     8821 tctggggcac caacaatacc gaccatcagg cgcgcatctg ccattcaacg accgtcaccg
     8881 gcgtagccaa tacgtggggc tacggcgcga tgaccaacag cttcaatgat atccgcaatg
     8941 ccaagaccca gatcatcatg ggcggcaatc cggccgag
//
LOCUS       DASWGM010000038         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2497255,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000038 DASWGM010000000
VERSION     DASWGM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497255"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VGA15_01365"
     CDS             complement(<1..1067)
                     /locus_tag="VGA15_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX9586380.1"
                     /translation="MTTCDEIETAENSSLWRIVKLLLFCVMQGAAVILVGFAALFLSF
                     EPVWSAENPSATLASLSDARSGSLLLKTDDGYADAIRLGIDVDLTVSGPTVRARVTQI
                     FRNPTPNWVEATYVYPLPAGGAVDTLKMVIGDRVVVGDIKERQQARIIYEQAQRNGQK
                     AALTEQERPNIFTNSVANIGPGETVLVQIEYQEPVQQMGNEFSLRVPMVVGPRYNPAP
                     VVQSVDFRPNGGGWGATSTDPVPDRNRISPPVLDPAEHGPVNPTAITVRLQAGFPLGE
                     VKSHNHQVKIDSPDDSTRVIRLAEGAVPADRDFELSWKPAAEKAPSVGLFREHVGDAD
                     YLLAFVTPPSVEQAEQKPLPR"
     gene            1254..2021
                     /locus_tag="VGA15_01370"
     CDS             1254..2021
                     /locus_tag="VGA15_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9586381.1"
                     /translation="MLTPDKQLSAEQSRQVAETIREEIARRRISRQSLAEQAKLSLST
                     LEKVLGGRRPFTLATTVRLEQALGVSLRKAQEAPPPAAPMINGDVSPDGLGSYSRRSV
                     EWLEGVYVTVRPSFGDKDAIYAYRTEIAWDAAVSSLVFREGERQDAAYTQFGEVAVPN
                     QSGHIYLITNRHGQHRLITVSRPRISGEMYGIITTLLAGRGSLLTPIAAPIALLRIEA
                     ILSPAFGRVSPGDVSYAIYREHLRRIAEEPFALFLPG"
     gene            2052..3704
                     /gene="pgm"
                     /locus_tag="VGA15_01375"
     CDS             2052..3704
                     /gene="pgm"
                     /locus_tag="VGA15_01375"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEX9586382.1"
                     /translation="MAVEVNPLAGKAIPPAMLVNVPRLVTAYFSNKPDPKIPSQRVAF
                     GTSGHRGSAFNNAFNEAHILAISQALCDHRRQSGIDGPLFMGIDTHALAECAFASALE
                     VFAANDVTVMIDERDGYTPTPAISHAILTYNKNRTTGLADGVVITPSHNPPEDGGFKY
                     NPPNGGPADTDVTTGIERAANAFLEDGLRGLHRISYHRAHKAKCVHRYDYIGPYVADL
                     ANVIDMEAIRASGVKIGIDPLGGAAVHFWQPIIERYGIAATIVNDTVDPTFRFMTADW
                     DGKIRMDCSSPYAMARLIGMRDKFDVAFANDTDADRHGIVTRSNGLMNPNHYLAAAIA
                     YLFENRPGWSKNSAVGKTIVSSAIIDRVATKLGRKLVETPVGFKWFVDGLIGGSFGFA
                     GEESAGASFARRDGSVWTTDKDGIILGLLAAEITARTQQDPSQLFDRLTGELGVPFYE
                     RIDAPATVAQKNLLKALSPEQLAIKELAGEPVRATLNAAPGNNQSFGGIKVVAENGWF
                     AARPSGTEDVYKIYAESFISQQHLRQIQQQAQAAITAAFEAK"
     gene            complement(3875..>4744)
                     /locus_tag="VGA15_01380"
     CDS             complement(3875..>4744)
                     /locus_tag="VGA15_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586383.1"
                     /translation="AVQKYHNAKKVPQLFLATGASKWNDPKEYPWTMGFQPSYRVEAR
                     IFAKYILKTKPDAKVAVFYANDDFGKDYVAGLKEIFGDKSPSIIVAEESYETTEPSID
                     SHIVKLKGTGADVFVNISTPKFAAQAIKKMAELEWKPMHVMTDVSISIGAVMKPAGLE
                     ASEGILSATYIKDASDSQWANDAGMKKFMAFIEKYSPGANISDANLVYAYAAAQTMVQ
                     VLRQCGDNLTRENVMKQAASLKDFTPDTLLPGVRINTSATDYAPIEQLQMMQFKGGKW
                     QMFGDVISAETGG"
BASE COUNT          873 a         1463 c         1469 g          939 t
ORIGIN      
        1 tcgcgcggca gcggcttttg ttcggcctgt tcgaccgacg gcggcgtgac gaatgccagg
       61 agataatcgg catcgccgac gtgctcgcgg aacagcccga ccgacggcgc cttctccgcc
      121 gcgggtttcc agctcagctc aaaatcgcga tccgccggca ccgcgccctc ggccagcctg
      181 atgacgcgcg tgctgtcgtc ggggctatcg atcttgacct gatggttatg gctcttgact
      241 tcgccgagcg ggaagccggc ctgcagccga accgtgatcg cggtcggatt caccggccca
      301 tgttcggcgg gatcgagcac gggcggcgag atgcggttgc ggtccggcac cggatcggtg
      361 gatgtcgcgc cccagccgcc gccgttgggc cgaaagtcga cgctctgcac gaccggtgca
      421 ggattgtaac gcgggccgac caccatcggc acccgcagcg agaattcatt gcccatctgc
      481 tgcacgggct cctggtattc gatctgcacc agcacggttt cgccggggcc gatattggcg
      541 accgagttcg taaaaatgtt cggccgctcc tgttcggtca atgcggcctt ttgtccgttc
      601 cgctgcgcct gctcgtagat gatacgggcc tgctgccgtt ccttgatatc gccgaccacg
      661 acgcggtcgc cgatcaccat tttgagcgtg tcgaccgcgc cgcccgccgg cagcggatag
      721 acataggttg cctccaccca gtttggcgtc gggttgcgga agatctgcgt gacgcgggcg
      781 cggacggtgg ggcccgatac ggtaagatcg acgtcgatgc cgagcctgat cgcgtcggca
      841 tagccgtcgt cggttttcaa gagcagggag ccggagcgcg cgtcgctgag gctggccagg
      901 gttgccgacg gattctccgc cgaccacacc ggctcaaagc tcaggaataa tgccgcaaat
      961 ccgaccagga tgacggcggc gccctgcatc acacaaaaca acagcagttt tacgatacgc
     1021 cataggctag agttctcggc ggtctcaatt tcatcgcacg tggtcatttc gcttaactcc
     1081 cgcgggggtt tcatcgcctg cggttgtgcg gaaccgcggg ttctggcgcg agcagaatga
     1141 tctgtaatgt tcagccgcaa gccgcttcgg cgcgccgcgg ccaaggcagt tttgtgcggc
     1201 tttgtgatga attgtgcgtc tgctaggatg gtggaattcg aaaaggggtg acgatgctga
     1261 cgccggacaa gcagctttcc gccgagcaaa gccggcaggt ggcggagacc attcgcgagg
     1321 aaatcgcgcg gcgtcgcatc tcccggcagt cccttgccga gcaggccaaa ctcagcctct
     1381 cgacgctgga aaaggtgctg ggcggccgcc gcccgttcac gctggccacc accgtacggc
     1441 tcgaacaggc gctcggcgtt tccttgcgca aggcgcagga ggccccaccg cccgcggccc
     1501 cgatgatcaa cggcgacgtc tcccccgacg gcctcggctc ttactcgcgc cgctcggttg
     1561 agtggctcga aggcgtctat gttacggtgc ggccgtcgtt cggcgacaag gacgcgatct
     1621 atgcctatcg caccgagatc gcctgggatg ccgcggtttc atcattggtg ttccgtgaag
     1681 gcgaaagaca ggacgcggcc tacacccagt tcggcgaagt cgcggtgccg aaccagtcgg
     1741 gccacatcta tctcatcacc aaccggcatg gccagcatcg cctgatcacg gtatcgcgcc
     1801 cgagaatatc aggcgaaatg tacggcatca tcaccacgct gctggcaggg cgcggctcgc
     1861 tgctgacacc gattgcggcg cccatcgcgc ttttgcggat cgaggcgatc ttgagtccgg
     1921 cgttcggacg ggtttcgcct ggtgacgtca gctacgctat ctatcgcgag cacctgcggc
     1981 gcatcgccga ggagccgttc gccttgttct tgccgggcta gagtctgatt caactgaaga
     2041 aggagagatc aatggccgtc gaagtaaacc cgctcgccgg caaagccata ccgccggcga
     2101 tgctggtcaa tgtgccgcgg ctggtgacgg cctatttctc aaacaagccg gatcccaaaa
     2161 tcccgtccca gcgcgtggcg ttcgggactt ccgggcatcg cggctcggcc ttcaacaacg
     2221 ccttcaacga ggcgcatatt ctggcgatca gccaggcgct ctgcgaccat cggcgccaaa
     2281 gcgggatcga cggcccgcta ttcatgggca tcgacacgca tgcgctggcg gagtgcgcct
     2341 tcgcaagtgc gctcgaggta tttgcagcca acgatgtcac cgtgatgatt gacgagcgtg
     2401 acggatacac gccgacgcct gcgatctcgc atgcgatcct gacctacaac aaaaatcgga
     2461 ccaccggcct tgccgatggc gtcgtgatca ccccgtcgca taatcctccc gaggatggcg
     2521 gcttcaaata taatccgcca aatggcggac cggccgacac cgatgtcacg acgggaatcg
     2581 agcgcgccgc gaacgcattt ctggaagacg gattgagggg cttgcaccgg atttcgtatc
     2641 accgcgccca caaggccaag tgcgttcacc gctacgatta catcggcccc tatgtggccg
     2701 accttgccaa cgtcatcgat atggaggcga tccgcgcctc gggggtgaaa atcgggatcg
     2761 atcccttggg cggcgcggcg gtccatttct ggcagcccat catcgagcga tacggcatcg
     2821 ccgcgactat cgtcaacgac acggtggacc cgacgtttcg gttcatgacg gccgattggg
     2881 acggcaagat ccggatggat tgctcatcgc cctatgccat ggcgcggctg atcggcatgc
     2941 gcgacaagtt cgatgtcgcc ttcgccaacg ataccgacgc cgaccggcat ggcatcgtca
     3001 cccgctccaa cgggctgatg aatccgaacc attatctcgc cgccgcgatt gcctatctgt
     3061 tcgagaatcg cccgggctgg agcaagaaca gcgctgtggg gaagacgatc gtcagcagtg
     3121 cgatcatcga tcgcgtggcc acaaaactcg gccgcaagct ggtcgagacc ccggtgggct
     3181 tcaaatggtt cgtcgatggc ctgattggcg ggtcgttcgg cttcgccggc gaggaaagcg
     3241 ccggcgcatc attcgcccgg cgcgatggct cggtctggac caccgacaag gacggcatca
     3301 ttcttggtct attggccgcc gagatcacgg cgcggaccca acaggacccc agtcagctat
     3361 tcgatcgtct caccggcgaa cttggcgtgc cgttctatga gcggatcgac gcccccgcga
     3421 cggtcgcgca aaagaatctg ctgaaggcgc tttcgccgga gcaactcgcc atcaaggagc
     3481 ttgccggcga gccggtgcgc gcgacgctga atgcggctcc cggcaacaat cagtcgttcg
     3541 gcggcatcaa ggtggtggcg gaaaacggat ggtttgccgc aagaccttcc ggaaccgagg
     3601 atgtctacaa gatctacgcg gaaagtttta taagccagca gcatctgcgg cagattcagc
     3661 agcaggccca ggccgcgatc acggcggcat ttgaggcgaa gtagaggacg cagcccttgt
     3721 gtctactttc cgtcattgcg agccaacggg tcgcgcgaat gcgcgcccga tgacaggctc
     3781 cacgaagcaa tccatctcgc cgcgaagaaa gtatggattg cttcgtcgct gcgctcctcg
     3841 caatgacgat ctgaaatagc gtatctgccc gctctcagcc ccccgtctcg gcgctgatga
     3901 cgtcgccgaa catttgccat ttgccgccct tgaactgcat catctggagc tgctcgatcg
     3961 gtgcgtagtc ggtggcgctg gtgttgatcc tcacaccggg aagcagcgtg tccggagtga
     4021 aatccttcag gctggcggcc tgcttcatga cgttttcccg ggtcaaattg tcgccgcact
     4081 gccgcagcac ctgcaccatg gtctgcgccg cggcataggc gtacaccaga ttggcgtcgg
     4141 agatgttcgc gccgggtgag tatttttcga tgaacgccat gaatttcttc atgccggcat
     4201 cgttggccca ctggctgtcg gacgcatcct tgatgtaggt ggccgacagg atgccttcgg
     4261 aagcctccag gccggcgggc ttcatgaccg cgccgatcga gatcgagacg tcggtcatga
     4321 catgcatcgg cttccattcc agctccgcca tcttcttgat cgcttgtgcg gcgaatttcg
     4381 gcgtcgagat attgacgaac acgtcggcgc cggtgccttt cagcttgacg atgtgggaat
     4441 cgatcgaggg ttcggtggtt tcatagcttt cctcggcgac aatgatgctg ggcgatttgt
     4501 cgccaaaaat ttccttcagc ccggcgacat agtccttgcc gaaatcgtca ttggcataga
     4561 acaccgcgac cttggcgtcg ggctttgtct tcaggatgta tttcgcgaaa atccgggcct
     4621 cgacgcggta gctcggctga aagcccatgg tccagggata ctctttcggg tcgttccact
     4681 tgctggcgcc ggtggcgagg aagagttgcg ggaccttctt ggcgttgtga tatttctgaa
     4741 ccgc
//
LOCUS       DASWGM010000039         4185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_417616,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000039 DASWGM010000000
VERSION     DASWGM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417616"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VGA15_01385"
     CDS             <1..436
                     /locus_tag="VGA15_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9586384.1"
                     /translation="MGALSHLKIVEIGSAATASYCARLFADFGATVQKVEPPSGDPLR
                     QAAPFTPKGHSAWFAFLNFNKSSVALDPNDASASLRLIELVRDCDILIDGRDIDEADC
                     PAFDLAALKLRAIRVSFTSKQAGSAGRVPMQILRRPTRPFVR"
     gene            355..2448
                     /locus_tag="VGA15_01390"
     CDS             355..2448
                     /locus_tag="VGA15_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9586385.1"
                     /translation="MHVEASWFGGEGPYANFEATDSTIRALTGLVKLVGPVEGPPVHA
                     PDFQTGIFGGLWGFVAAAASVLSRTHDGRGRTSRLSIFESSIAATEYIMFEAFLRGDI
                     MRRIGVNRFWPTFPVGIYETKRGWLGVTTVTPAQWRAFCEMLGLFDLRDDATLFMGVD
                     RLQRMEEIESKFGPKLKQRTAQEWFAEGLKRKIPIVPVPGISDLINDEEKRSRGAIVP
                     IVIGDEAGFTAGSMQRLTGTPPRRGGAVPAIGESQPVAGRSSDGGGRALAPRPDDSSR
                     LPLEGVRVIDFSMGWAGPICTRTLADLGADVIKIEAIQYPDWWRGVDRRPAYVLEQMY
                     EKSVRYCIMNRNKRGITLDLTRPRGLHLARQLLTDADLVVDNYSVEVLPKLGLGYEVL
                     STLNPKLVMMSMSAFGAGSIHRDCRAYGSTLEQGSGLPNVVGDANGPPVMSHTAFGDA
                     VGGLNGCAAVLTALIYAKLTGKGQFIDLAQIECMMPFAAPWIVAHSIDGKAPVKYGNR
                     HPDFVPHGCFPCAGSDNWIVVAVSSDVMWPRLCKLLGRADWAADETLGSAAGRRQIES
                     EIEEAITHWTSTRAPDDAMNALQAVGVASGVARLPIELLRDPQLLARGFIQEIDRAFI
                     GRHPQPSMPFREIDKPFVIRNPPPTLGEHNREILGGILGLSNGEIEELARDGIIGTEM
                     LMEEQLVKEKKRATS"
     gene            2448..3530
                     /locus_tag="VGA15_01395"
     CDS             2448..3530
                     /locus_tag="VGA15_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586386.1"
                     /translation="METARPAEYQSGAATSGSPQALMSVRGVGIRFKTAQGEWQATRK
                     IDFDIASGERVGIVGESGCGKTITGLSILRLLPNNLARLDGSIMLDDIDLASCSTRVM
                     RAIRGRRIAMIFQEPMSALDPVFTVGHQVAETLRVHTDIGKQEARAQTVEMLRRVGIA
                     SPERRIDDYPHQLSGGMRQRVMIAAALICGPQLLIADEPTTALDVTVQAQILELLRSL
                     SETSKTALMLITHDLGVVAETCTRMITMYAGEIIEDAAVDAALVRPLHPYTSGLLRSL
                     PHLSPRLCKLPSIPGRLPSLADMPDGCRFRSRCPHAIAGCEAEQPLLDAGDGRRVRCW
                     RFRELELPGALQHAATAPIAAMVARQ"
     gene            3530..>4185
                     /locus_tag="VGA15_01400"
     CDS             3530..>4185
                     /locus_tag="VGA15_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9586387.1"
                     /translation="MPPAADTKVDPIVSVRDLEVCFQTTDRRATVKAVDGVNFDVRRG
                     ETFGIIGESGSGKTTIGRALVFLLKPTAGAILHNGIDPQSLPRKEFQSHRRDYQIIFQ
                     DPNAALNPRMTIVSSVLEPLELARAGTTAERLGRAHDAMERVGLPKEIGERYPHQLSG
                     GQMQRVIIARAPTLRPKLIVCDEVVAALDMSIRGDVLNLFAELQRDLGLTYVFITHDL
                     S"
BASE COUNT          803 a         1248 c         1308 g          826 t
ORIGIN      
        1 gatgggggcg ttgtcgcatc tcaaaattgt cgagatcggg agcgccgcga cagcgagcta
       61 ttgcgcacgc ttgtttgcgg acttcggtgc gaccgttcag aaggtcgaac cgccgtcagg
      121 cgatccgctt cgccaggctg cgccgttcac tcctaaagga cacagcgcgt ggtttgcgtt
      181 tctcaatttc aacaagtcga gcgtcgcact cgatccaaac gatgctagtg cgtcgttgcg
      241 cctgatcgag ctggtgcgcg actgcgacat cctgatcgac ggccgcgata tcgatgaggc
      301 ggactgcccg gcattcgatc tggccgcact caagctcaga gcaatccggg tctcattcac
      361 gtcgaagcaa gctggttcgg cggggagggt ccctatgcaa attttgaggc gaccgactcg
      421 accattcgtg cgctaaccgg attggtaaaa ctggtcgggc ctgtcgaagg accgccggta
      481 catgcacccg atttccaaac cggcatcttc ggcggcctct ggggcttcgt tgcagcggcg
      541 gcgtcggtgc tgagtcgcac gcacgatgga cgcggtcgca ccagcaggct gtccatcttc
      601 gaatcaagca ttgcggccac cgaatacatc atgttcgagg cgttcctgcg tggggacatc
      661 atgcgccgga tcggcgtcaa ccgcttctgg ccgacatttc ccgtcggtat ctatgaaacc
      721 aagcgaggct ggcttggcgt taccacagtg acccctgcgc aatggcgtgc cttctgcgag
      781 atgctggggc tgttcgacct gcgcgacgac gcgacgctgt ttatgggtgt cgatcggctg
      841 cagcgcatgg aagaaatcga gagcaagttt ggtccgaagt tgaagcagcg caccgcgcag
      901 gaatggtttg cggaagggtt gaagcgaaaa attcccatcg tgccggtgcc aggcatcagc
      961 gatctgatca acgacgaaga aaagagatcg cgcggcgcga ttgtcccgat cgtgatcggc
     1021 gacgaggccg gctttaccgc tggttcaatg cagcgactga ccggcacgcc gccgcgacgc
     1081 ggcggggcgg ttcctgcgat cggcgaatcg caacccgtcg ccggtcgttc cagtgatgga
     1141 ggcgggcgcg ctcttgcgcc gcggccggac gattcgagta ggcttcctct cgaaggcgtg
     1201 cgtgtcatcg acttctcgat gggttgggcc ggtccgattt gcacgcgcac gctggccgat
     1261 ctcggcgccg atgtgatcaa gatcgaggcg atccaatatc cggattggtg gcgcggcgtc
     1321 gatcggcgcc ctgcctacgt cctcgagcag atgtacgaga aatcggtgcg ctactgcatc
     1381 atgaaccgca acaagcgggg catcacgctc gatctgacgc ggccgcgggg cttgcatctt
     1441 gccaggcaac tgctgaccga cgcggatctc gtcgtcgaca attattccgt cgaggtactg
     1501 ccgaaactgg gactcggcta tgaggtgctg agcacactca atccaaagct cgtgatgatg
     1561 tcgatgtcgg ccttcggcgc aggcagcatc catcgcgatt gtcgcgccta tggctcgaca
     1621 ctcgagcagg gctcgggttt gccgaacgtc gtcggcgatg ccaatgggcc gcccgtcatg
     1681 agccacacgg cgttcggcga cgccgtcggc ggattgaatg gatgcgccgc cgtgctcacc
     1741 gctctgatct acgcaaagct taccggcaag ggccagttca tcgatctcgc gcagatcgaa
     1801 tgcatgatgc ctttcgccgc tccctggatc gttgcgcatt cgatcgatgg caaggcgcct
     1861 gtgaaatacg gcaaccgtca tccagacttc gtgccgcatg gctgcttccc atgcgctggt
     1921 agcgacaact ggatcgtcgt cgccgtttcc agcgacgtga tgtggccaag actctgcaag
     1981 ttgctgggcc gcgcggactg ggccgccgat gaaacgttag gaagcgccgc cggacggcgc
     2041 caaatcgaga gcgaaatcga agaagcaatt actcattgga cctctacgcg cgctcccgac
     2101 gacgcgatga acgcgttaca ggccgtcggg gtcgcatccg gcgttgcgcg gttgccaatc
     2161 gaattgctga gagatccgca attgctcgca cgtggattca ttcaggagat tgatcgcgcc
     2221 ttcatcggtc ggcatccgca accgtccatg ccgtttcgcg agatcgacaa gccgtttgtt
     2281 atccgtaatc cgccaccgac gctcggcgag cacaaccgtg aaatactcgg cggcattctt
     2341 ggactgtcca atggcgagat cgaggaactt gcgcgtgacg gcattatcgg aaccgagatg
     2401 ctgatggagg aacaactcgt gaaagagaag aaacgggcga caagctgatg gaaacagccc
     2461 gcccggcaga ataccaatcg ggagcggcaa cgtctggcag cccgcaagcg ctgatgtcgg
     2521 tgcgcggggt tggtattcga ttcaagaccg cgcaggggga atggcaggca acgcgcaaga
     2581 tcgatttcga cattgcttcg ggcgagcgcg tcggcatcgt cggcgaaagc ggttgtggca
     2641 aaaccatcac gggcctttcc atcctgcgcc tgttgccgaa caaccttgcc cgtctggacg
     2701 gctcgatcat gttagacgac atcgatcttg cttcctgcag cacgagagtg atgcgcgcga
     2761 tacgcggccg gcgcatagcg atgatctttc aggagccgat gagcgcactc gatccggtgt
     2821 ttaccgtcgg acatcaagtc gccgagacgc tgcgcgttca caccgatatc ggcaagcagg
     2881 aagcgcgggc gcagaccgtc gagatgcttc gccgtgtcgg cattgcctcg cctgaacggc
     2941 gcatcgacga ctatccgcat cagctctccg gtgggatgcg tcagcgggtc atgatcgccg
     3001 ctgcgctaat ctgcgggccg cagctgttga tcgccgatga gccgaccaca gcccttgatg
     3061 tcacggtgca ggcgcagatt ctcgaattac tgcggagtct cagcgagaca tcgaaaacag
     3121 cgctgatgct gatcacacat gaccttggcg tcgtcgccga gacctgtacg cgaatgatca
     3181 cgatgtatgc aggcgagatc atcgaggatg cggctgtcga cgccgcgctg gtgcggccgc
     3241 tgcatcccta tacgtccggc ctgctgcgct cgctgcctca cttaagcccg cgtctttgca
     3301 agcttccttc cattccgggc cgcctgccat cgcttgccga tatgcctgac ggttgccgct
     3361 tcaggtcgcg ttgtccgcat gcgatcgcgg gctgtgaagc cgaacagcca ttgctcgatg
     3421 caggcgacgg tcgcagggtt cgctgctggc gcttcaggga actcgaattg ccgggcgcat
     3481 tgcaacacgc agccaccgcg ccgattgcgg caatggtagc ccggcaatga tgcctccggc
     3541 cgctgacacg aaggtggatc cgatcgtttc ggtacgtgac cttgaggtgt gctttcaaac
     3601 gacggatcgg cgcgcgacgg tgaaagcggt cgatggcgtc aatttcgatg tccgtcgcgg
     3661 cgagacgttt ggcattatag gagaatccgg atcgggcaaa accacgatcg ggcgcgcgct
     3721 ggtttttctg ctcaagccga ccgcgggcgc cattttgcac aatggtatcg atccgcaaag
     3781 cctgccgcgg aaagagttcc aaagccatcg ccgtgactac cagatcatct ttcaggatcc
     3841 caacgcggcg ctaaatccac gcatgacgat cgtctcgtcc gtgcttgaac cgctcgagct
     3901 cgcgcgcgca ggcaccacgg cagagcgctt ggggcgcgcg cacgatgcca tggagcgcgt
     3961 cggcctgccc aaagagatcg gcgagcgata tccgcaccag ctttccggcg gtcaaatgca
     4021 gcgcgtgatc attgcgcgag cgccgacgct tcggccgaag ctgatcgttt gcgacgaggt
     4081 ggtggcggcg ctcgacatgt cgatccgcgg cgacgtgctc aatctgttcg ccgagctgca
     4141 acgcgacctc ggtctcactt acgttttcat aacccacgat ttgtc
//
LOCUS       DASWGM010000040        12279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1387719,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000040 DASWGM010000000
VERSION     DASWGM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12279
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387719"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VGA15_01405"
     CDS             complement(<1..608)
                     /locus_tag="VGA15_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEX9586388.1"
                     /translation="MRELFDEVAGKSPLDPEEVVRRTTRGPRRKRFYAHAGVVDAPDG
                     LAITLDDRPVRTPSGRPLVAPTREIADAIVTEWDSQKEFIDPLTMPLTRFANSVVDAV
                     VDRVEAVADDIAKYLGSDLLFYRAGHPEALVAREAALWDPVVFWAADALGAHFILAEG
                     IVHVRQPDTAIAAARAALPADPWSIAALHVVTTLTGSALLALA"
     gene            703..1152
                     /locus_tag="VGA15_01410"
     CDS             703..1152
                     /locus_tag="VGA15_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586389.1"
                     /translation="MARVFATILVLLADFGLSTVEAGFHSPESLVRNVYAYYGDRSSE
                     LSNGLPRDPDTASQFFDKGLRAAWISVKDQPYDFLVQSPTWKLGAVSISILRRQFDKT
                     YVAVAFDNNGRAVTLNFIVVNGPDGWVISDVESPHDSLRMFLAQYRH"
     gene            complement(1164..1472)
                     /locus_tag="VGA15_01415"
     CDS             complement(1164..1472)
                     /locus_tag="VGA15_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586390.1"
                     /translation="MKNLSILICAVAAALGAADAALAQVMIPPGGSQFNPPLPPPPPP
                     PKIEPPVIPQLDAPLRQNPTPAPHPSFSDRISRCLDEGAASGLGPNERAAYSRSCANR
                     "
     gene            complement(1604..2827)
                     /locus_tag="VGA15_01420"
     CDS             complement(1604..2827)
                     /locus_tag="VGA15_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9586391.1"
                     /translation="MIGNLLRAALFLFLFSMAACFSVAANAQTLALLGGRVYPSPDAA
                     PLVDAVVLTSGGAITAIGSRSEVQVPNDARVIDCTGKTVVAGFWNSHVHFTQAVWKNA
                     ASAPVASLEEHMREMLTQWGFTTVWDLGSDPNDSLPLRRRVNSGEVAGPSILLAGDIF
                     PKGGHPVYLPPEMQLPEAATSEEAAQLARDDLRIGMDGMKLFTGVYMGSKPVVNMDAA
                     IAKAAIDVAHAQGKPVFAHPQNRTGVETVIAAGVDVLAHIVPGEPSYAPEQLARFKSQ
                     GIALIPTLSLFATSVGGPALAARLVESGVNQLKGFSENGGPVLFGTDVGFTTVYDTSL
                     EYELMHRALSESQVLASLTTNPALYFKAAKKGKVEKGFDADLVVLDGDPIADVRNLAK
                     IAYTIRAGQVIYHRP"
     gene            complement(2984..4180)
                     /locus_tag="VGA15_01425"
     CDS             complement(2984..4180)
                     /locus_tag="VGA15_01425"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9586392.1"
                     /translation="MSRRVKRPLAKRASGDRPKGARPFRGSQAERQPGHRAGGKPAPR
                     FAASPRPAPLIEAPEKIVAELPPLPTKVQTVVVTADENNMRVDRFLEARFPGLSFSHI
                     QRIVRKGELRVNGKRADSKDRLEEGQNVRIPPLKLDAPKVAGSLSEAATKTLQALKDM
                     ILYEDADVMVLNKPAGLAVQGGSGTTRHVDQMLEAMRDAKGQKPRLVHRLDKETAGCL
                     LVAKTRFAATALTGSFRHRSARKIYWALVAGVPKPKQGRISTYLAKDESEDDSIMRIA
                     AHGDEGASHAVTYYAVVETSAQKLAWVSLKPVTGRTHQLRAHMAHIDHAIVGDPKYFN
                     KENWQLPGGLQKRLHLLARRIVIPHPRGGFIDATAPLPPHMLQSWNLLGLEADRFDPI
                     ENAPEE"
     gene            complement(4177..5037)
                     /locus_tag="VGA15_01430"
     CDS             complement(4177..5037)
                     /locus_tag="VGA15_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586393.1"
                     /translation="MMPFDSFVGIDWSGDKKPWQKGLKVGVAYPGRAAPQLVECPSSK
                     RGWSRTEVMRWIEDEFQDKRALIGIDFAFGFPPVTLPGNIVLDWDYVEEICASYPNFY
                     GGAFFRPPACAHSHLINSPWLPRTGYSAGHLRLTDLIAKNTIGATPQPIFNAVGPAQV
                     GPSSISGMRALRSLRQNCGDGITIWPLSEASPTGSIIVEIFPRYFPLSKGKGARLADL
                     NNLNPALAAFDSDGLKDGPKSEDEGDALLSAAALRSLSIQESLFQLPDQAIRSQGWIF
                     GVPVQNGKSV"
     gene            complement(5034..6374)
                     /locus_tag="VGA15_01435"
     CDS             complement(5034..6374)
                     /locus_tag="VGA15_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX9586394.1"
                     /translation="MSPKRPREATNLFAAAGMEQDAPHPLPDRLRPRALSDVVGQDHI
                     LGPDGALTRMLATRTLGSLIFWGPPGTGKTTVARLLADATELHFEQISAVFSGVADLK
                     KVFDAARARREMGKGTLLFVDEVHRFNRAQQDSFLPVMEDGTVVLVGATTENPSFELN
                     AALLSRARVLVFHSLDPAAIEKLFAHAEKIEAKKLPLDAEARAVLVRMADGDGRAALT
                     LAEEVWRAAREGETFNAEALQDILQRRAPIYDKSADGHYNLISALHKSVRGSDPDAAL
                     YYLARMMDAGEDPLFLARRVVRMAVEDIGLADPQALVICSAAKDAYDFLGSPEGELAI
                     AQAVIYLATAPKSNAAYKAFGAAMRAAKEGGSLLPPKHILNSPTKLMKSEGYGSGYEY
                     DHDTQDAFSGQDYFPEAMGRQTFYDPPDRGFEREIRKRLDYWAKLRAGRTGPDR"
     gene            complement(6371..7780)
                     /locus_tag="VGA15_01440"
     CDS             complement(6371..7780)
                     /locus_tag="VGA15_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX9586395.1"
                     /translation="MHRFVRTAVLPAIWAVTLLGSLPCAIAQDRRVPASPAEVKLSYA
                     PIVQRVQPAVVNVYAAKMVQNRNPLLDDPIFRRFFGVPGQQPEQMQRSLGSGVMVDPS
                     GLVVTNVHVIEGADEVKVSLSDKREFEAEIVLKDPRSDLAVLRLKGVQEKFPTLDFAN
                     SDELLVGDMVLAIGNPFGVGQTVTHGIISALARTQVGITDYQFFIQTDAAINPGNSGG
                     ALVDMTGRLAGINTAIFSRSGGSQGIGFAIPANMVRVVVASAKSGGKAVKRPWLGARL
                     QAVTPEIAESLGLRLPTGALVASVVASSPAARSGLKASDLIVAIDGQAVDDPNGFDYR
                     FATHPLGGTSQVDVQRGGKTVKLTIPLETAPDTGRNEIVLTTRSPFQGAKVANISPAV
                     ADELHLDADIEGVVVTDLADGATAASVGFQKGDIIMSVNNQKIAKTSDLEKATRESAR
                     LWRITVVRGGQQINVTLGG"
     gene            8076..9665
                     /locus_tag="VGA15_01445"
     CDS             8076..9665
                     /locus_tag="VGA15_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OprO/OprP family phosphate-selective porin"
                     /protein_id="HEX9586396.1"
                     /translation="MTTPRLSTSVALSLVGALMASQAHAQSTGSSDAEIALLKQQLRL
                     MEQKLDNLQKQTTANTAAAAKANAKADAKVTVANANAAYPVKGPGAPSDVVVKMPNNR
                     PTICTADDQNCISITSRVHFDVGGYDYHPNSAATVPQRLDDGVNVRRARIGVLGKFLG
                     DWNYALIYDFGGASDGFASTASVGGTAVGFLPGGALSGIENAYLSYTGFKPFGGKLAI
                     EGGVMDLPYTLDEASSSNDIPFMERASSGITATNIAAGDFRSAFGARWYTDTFWAGAY
                     VTGPATGAIHSASSLNPNGTTEQYGTVARAAGQIVSGKDYSLHIGGEAEWLLQPPHNL
                     IAGTQTLTLSDRPEVRIDPTTLVSTGAIAGVSGAQVYSAEVAGTYGPLFFQGEYFWYN
                     VDRNAFAPLPSLKFQGGYAEAAYVLTGESRPYNPGSASYGGIAPANPFSLSGDGWGAW
                     EIAGRYSTIDLNDQIATASGVAGGRQTIYTLALNWYLNRNVRLMFDYLHGDVAKQVSP
                     TNFADTGSKFNAFAMRTQVAF"
     gene            complement(9782..10150)
                     /locus_tag="VGA15_01450"
     CDS             complement(9782..10150)
                     /locus_tag="VGA15_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9586397.1"
                     /translation="MKRRNFAHRPGCAVEATLDLIDGKWKGVILYHLQDGTQRFGELR
                     RRMPGITQRMLTKQLRALEDDHLVIRKVYAEVPPRVEYRLSEIGESLRPVIDTLKAWG
                     EGHREKLSCAPVSDVPDRAA"
     gene            10275..11288
                     /locus_tag="VGA15_01455"
     CDS             10275..11288
                     /locus_tag="VGA15_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9586398.1"
                     /translation="MKAVGYKKSLPVDEADSLIDFETAKPEPKGRDIRVAVKAISANP
                     VDYKVRKRAAPPEGETKILGYDAAGIVDAVGPDVTLFKAGDEVFYAGSIQRQGTNSEF
                     HLVDERIVGQKPKTLSFAQAAALPLTSITAWELLFDRLGAVSGKSVDPRTLLIIGGAG
                     GVGSILIQLARRLTGLTVVATASRPESQKWCLDLGAHAVIDHAKPMKEQIEKLKLSPV
                     ALVASLTNTDQHYKALADIIAPQGKYGLIDDPPEFNVSVFKGKAVSIHWESMFTRSSF
                     QTADMIGQHALLNDIADLIDKGVLRTTLDQAFGTINAANLKRAHALLESGKSRGKIVL
                     EGW"
     gene            complement(11359..11655)
                     /locus_tag="VGA15_01460"
     CDS             complement(11359..11655)
                     /locus_tag="VGA15_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX9586399.1"
                     /translation="MSAYVISELEVRDAAAIETYRTIAAKSIAQYGGRYLVRGGAASI
                     AEGGPPPKNIVVVEFPSMERLREWYASPEYAEALKVGATALDRRLIFVEGVPAV"
     gene            complement(11664..>12279)
                     /locus_tag="VGA15_01465"
     CDS             complement(11664..>12279)
                     /locus_tag="VGA15_01465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9586400.1"
                     /translation="AGVAADVSTAAGCDTLVEMLPDVDILINNAGIFEPKGFFDIADD
                     DWSRFFEVNVMSGVRLSRAYLPGMLKRNWGRIVFISSESALNIPKEMIHYGMSKTAQL
                     AVSRGLAEMTRGTAVTVNSVLPGPTMSEGVEAFVKDLARQNGQSVDEAASQFVKQFRP
                     TSLLQRFATVEEIANMVVYVASKEASATNGAALRAEGGIVQTIA"
BASE COUNT         2419 a         3819 c         3709 g         2332 t
ORIGIN      
        1 gccaaggcga gcagcgccga gcccgtcaat gtcgtcacca cgtgcaaggc tgcgatcgac
       61 caggggtccg ccggcagcgc cgcgcgcgcc gccgcgatcg cagtatccgg ctggcgcaca
      121 tgcacgatcc cttcggccaa aataaaatgc gcacccagcg catccgcagc ccaaaacacc
      181 accggatccc aaagcgcggc ttcccgcgcc accagcgcct ccggatggcc ggcgcggtag
      241 aacaaaagat ccgacccgag atatttcgcg atgtcgtccg cgaccgcttc gacccgatcg
      301 accacggcat caacgacgct gttggcgaac cgggtcaacg gcatggtcag gggatcgatg
      361 aattccttct ggctgtccca ttccgttaca atggcgtcgg cgatctcccg ggtcggcgca
      421 accagcgggc gcccggaagg cgtgcggacc ggcctgtcat cgagggtaat ggccaaacca
      481 tccggcgcgt cgacgacgcc cgcatgggcg taaaaacgct tgcggcgcgg cccgcgcgtg
      541 gtgcgccgta ccacctcctc cggatcgagc ggagactttc cggcgacttc atcgaacagt
      601 tcacgcattc ggttttcagc tcatgtccgt caatggtaat ttgaccgcca gcgtacgaca
      661 aggacagccg caaataaagc gagccggcca agcgagcctg caatggcgag ggttttcgca
      721 accatcctgg ttctactggc cgacttcggt ctctcgaccg tcgaggctgg tttccattcg
      781 ccggaatcgc tggtgcggaa cgtctacgcc tattacggcg acaggtcgtc cgaactctcc
      841 aacgggctgc cgcgcgaccc cgacaccgca agccagttct tcgataaagg tttgcgggca
      901 gcatggattt cggtgaagga tcagccctac gatttcctgg tgcagagccc gacctggaaa
      961 cttggcgcag tctcgatatc gatcctgcgc aggcaattcg acaagaccta tgtcgccgtc
     1021 gcttttgaca ataacggccg cgccgtcacg ctgaatttca ttgtcgtcaa tggtcccgat
     1081 ggctgggtga tttccgacgt cgagagcccg catgattcat tgcggatgtt tctcgcccaa
     1141 taccggcact aagagagttc ctgtcatcga ttggcgcagg accgcgaata ggccgcgcgc
     1201 tcgttcgggc cgagcccgct ggcggcgcct tcatcgagac atctgctgat ccggtcgctg
     1261 aacgaaggat gcggcgcggg cgtggggttc tggcgcaacg gcgcgtcgag ctgcggtatc
     1321 accggcggct cgattttggg cggcggcggg ggtggaggca gcggcggatt gaattgcgac
     1381 ccgccgggcg gaatcatcac ctgcgcgagc gcggcatccg cggcacccag cgcggctgcg
     1441 acggcacaga tgaggatcga taaattcttc atggagggta tgtcgtgatc atcccggcaa
     1501 accgcaagct gaaattattc ttccggcgcg ttctcgatag gatgacagca atggccgcgc
     1561 gctcacactg aatgatgggc caattcggaa cggcagcagc ctttcacggt ctgtgataaa
     1621 tgacttggcc ggcacggatg gtgtaggcga tcttggcgag gttgcgaacg tcggcgatgg
     1681 gatcgccatc aagaaccacg agatcggcgt caaatccctt ttcgacctta cccttcttcg
     1741 cggccttgaa atacagggcg gggttcgtcg tgagggaagc gagcacctgg ctttccgaaa
     1801 gcgctcggtg catgagttcg tattcgagcg atgtgtcgta gaccgtggtg aagccgacgt
     1861 cggtcccgaa gagaacggga ccaccatttt ctgaaaatcc cttgagctgg ttgacgccgg
     1921 actcgaccag ccgggcggcc aaggcgggac cgccgacact ggtcgcaaaa agagatagcg
     1981 tcggaatcaa tgcgatgcct tgcgatttga accgggcgag ttgctcgggg gcgtaactcg
     2041 gttctccggg aacgatgtgg gcgagcacat cgacgccggc tgcaatcacc gtttccacac
     2101 cagttctgtt ttgcggatgg gcgaacaccg gcttcccctg tgcatgcgcc acatcgatcg
     2161 ccgccttggc gatggccgca tccatattca cgacgggctt gctgcccatg taaacgccgg
     2221 taaagagctt catgccgtcc atgcctatgc ggaggtcatc gcgtgccaat tgcgcggctt
     2281 cctctgatgt agcggcctcg ggaagttgca tctcgggagg caaataaacg ggatgaccgc
     2341 ccttggggaa gatgtcgccg gcaagaagga tactgggacc cgcgacctca ccagagttga
     2401 cgcggcggcg gagcggcaag gagtcgttcg ggtcggagcc aagatcccaa acggtggtga
     2461 aaccccactg cgtcagcatc tcccgcatat gctcttcgag cgacgcaacc ggcgcgctgg
     2521 ccgcgttttt ccataccgct tgcgtgaaat gaacgtgact attccagaag ccggcgacga
     2581 cagtctttcc agtgcagtca atgacccgcg catcgttcgg gacctggact tcgctgcgac
     2641 tgccgatcgc ggtgatggcg cccccagatg tcaggacgac ggcatccacg agcggggcgg
     2701 catcgggcga tggatatacc ctgcctccca agagagcgag tgtctgtgcg tttgcggcga
     2761 cgctgaagca agccgccatg ctgaagagga aaaggaaaag cgcggcgcgg agcagatttc
     2821 cgatcatggg cggagccttg aactttgcat gtcggcgcct cgaagcgttg aggaaaggcg
     2881 cccgtcggac cgaaccatat ccgatcagca cgacctcgct ttgatagttc ttgctccgct
     2941 cggacgatat tggataacgg cgcattgacg ccgcaacgcc ctgttattct tcaggcgcgt
     3001 tctcgatcgg gtcgaacctg tcggcctcca ggcccagcag gttccatgat tgcaacatat
     3061 gcggcggcag cggcgcggtg gcgtcgatga atccgccgcg cggatgcggg ataacgatcc
     3121 ggcgtgccaa aagatgcagc cgtttctgca ggccccccgg cagctgccag ttttccttgt
     3181 tgaaatattt gggatcaccg acgatggcgt gatcgatatg ggccatgtgc gcacgcagct
     3241 ggtgagtccg gccggtgacc ggtttcaacg acacccaggc cagcttctgc gccgaggtct
     3301 ccaccacggc ataataggtg acggcatggc tggcgccctc gtcgccatgg gcggcgatcc
     3361 gcatgatgct gtcgtcctcg ctctcatcct tggcgagata ggtcgaaatg cggccctgct
     3421 tcggcttcgg tacgccggcc accagcgccc agtagatctt gcgcgcggag cgatgacgga
     3481 acgatccggt cagcgccgtc gcagcgaagc gcgttttcgc gaccaggaga cagccggcgg
     3541 tctccttgtc gaggcgatgc accagccgcg gcttctgtcc cttggcatcg cgcatcgctt
     3601 ccagcatctg gtcgacgtgc cgcgtggtgc cggaaccgcc ttgtaccgcc aggccagcgg
     3661 gcttgttcag caccatgaca tcggcatcct cgtagaggat catgtccttg agggcttgca
     3721 gcgtctttgt cgccgcttcc gaaagacttc cagctacctt gggcgcatcg agtttcagcg
     3781 gcggaatgcg aacgttttgc ccctcttcca gccggtcctt gctgtcggcg cgcttgccgt
     3841 tgacgcgcag ctcgcctttg cggacgatgc gctggatgtg ggagaacgac aaaccgggaa
     3901 accgcgcctc gagaaagcgg tcgacccgca tgttgttctc gtcggcggtg acgacgacag
     3961 tctgcacttt tgtgggcagc ggcggcagct cggcaacgat cttctcgggc gcctcgataa
     4021 gcggcgccgg cctcggcgat gccgcaaaac gcggcgcggg tttaccgccc gcgcgatgcc
     4081 ccggctggcg ctcggcttgc gagccccgga agggccgcgc gcccttgggg cggtcgcccg
     4141 acgcgcgttt cgccaagggt cttttgacac ggcggctcat acggatttcc cattctgcac
     4201 ggggacacca aaaatccacc cttgcgatct gattgcctgg tcaggaagct gaaacaggga
     4261 ttcctgaatc gataaagatc taagagccgc agcgctgagc aacgcatctc cttcgtcttc
     4321 ggatttcggc ccgtccttca acccatcgct atcgaacgca gccagagctg ggtttagatt
     4381 attcaagtcg gcaagtcttg cgcctttgcc cttcgatagg ggaaaatagc gcggaaagat
     4441 ctcaacgatg atcgaaccgg tcggactggc ttcactaaga ggccaaatgg ttattccatc
     4501 gccgcagttc tgccgaaggg atcgcagcgc ccgcattcct gaaatcgaag agggcccaac
     4561 ctgtgccgga ccaaccgcat tgaagatcgg ctgaggcgtg gcgccgatcg tgttcttggc
     4621 gatcaagtcc gtcagtctca agtgaccggc tgaatagccc gttcttggca accaaggact
     4681 atttataaga tggctgtgtg cgcaagctgg tggacgaaag aacgcgccgc catagaaatt
     4741 ggggtacgac gcgcagatct cttcaacata gtcccaatcg agaacaatgt tgccaggaag
     4801 cgttacgggt ggaaatccga aggcaaagtc aatgccgatt aacgctctct tatcttggaa
     4861 ttcatcttca atccatctca tgacttcggt gcgtgaccac ccgcgtttac tacttgggca
     4921 ttctactaat tgaggcgcgg cgcggccggg ataagcaacg ccaactttaa ggcccttttg
     4981 ccacggcttt ttgtcaccgg accagtcgat gccaacgaag ctatcaaacg gcatcatcga
     5041 tctggtcctg tgcggccagc gcgcagcttg gcccagtagt ccagccgttt tcgaatctcg
     5101 cgctcaaacc cgcggtcggg cggatcgtaa aaagtctggc gtcccatcgc ttccgggaaa
     5161 taatcctggc cggagaacgc atcctgggtg tcgtgatcgt attcgtagcc cgagccatag
     5221 ccttccgact tcatcagttt ggtcggcgaa ttcagaatat gttttggcgg cagcagcgaa
     5281 ccgccctcct tggccgcgcg catcgccgcg ccaaaggcct tgtaggcggc gttcgatttc
     5341 ggcgcggtgg cgagataaat caccgcctgc gcgattgcca gttcaccttc gggagacccc
     5401 aggaaatcat aggcgtcctt ggcggcgctg cagatcacta gcgcctgcgg atcggcaagt
     5461 ccgatgtcct cgaccgccat gcgaacgacg cgccgcgcca gaaacagcgg atcctcgccg
     5521 gcgtccatca tccgcgcgag ataatacaaa gcagcatcgg gatcggagcc gcgcaccgat
     5581 ttatgcagcg ccgagatcag gttgtaatgg ccgtcggcgg acttgtcgta gatcggcgcc
     5641 cggcgctgca gaatgtcctg caaggcttcc gcattgaagg tctcgccttc gcgcgcggcg
     5701 cgccagacct cttcggccag cgtcagcgcg gcgcgcccgt cgccatcggc catgcgtacc
     5761 agcacggcgc gcgcctccgc atcgagcggc agctttttgg cctcgatctt ctcggcatgg
     5821 gcaaacagtt tttcaatcgc cgccgggtcg agcgaatgaa acaccaaaac ccgcgcccgc
     5881 gacagcaaag ccgcgttgag ctcgaacgag ggattttcgg tggtcgcgcc gaccagcacc
     5941 acggtgccgt cttccatgac aggcaaaaac gaatcctgct gcgcgcggtt gaaccgatgc
     6001 acctcatcga cgaacaagag tgttcccttg cccatttcgc ggcgcgcccg agccgcgtcg
     6061 aaaacttttt tcaggtccgc gaccccggaa aacaccgcgg aaatctgctc gaaatgcagt
     6121 tcggttgcat ccgccaataa tctcgcaacc gtggtcttgc cggttcccgg aggcccccag
     6181 aaaatcagcg aacccagcgt gcgcgtggcg agcatgcgcg tcagcgcgcc gtccggcccc
     6241 agaatatgat cctgcccgac gacatcggaa agcgcccggg gacgcaaccg gtccggaagc
     6301 ggatgcggtg catcctgctc catgcctgcc gcagcaaaca ggttggtggc ttcacgtggt
     6361 cgcttcgggc tcatccgccc agcgtcacgt tgatctgctg gccgccgcgc accacggtga
     6421 tgcgccagag ccgcgcggac tcgcgtgtcg ccttctcgag atcgctggtc ttggcgattt
     6481 tctgattatt gaccgacatg atgatgtcgc ccttttggaa cccgacgctg gcggcggtgg
     6541 cgccatccgc cagatcggtc accacgacgc cttcgatgtc cgcatccaga tgcagctcat
     6601 cggcgaccgc gggcgagatg tttgcaacct tggcgccctg gaacggcgag cgtgtcgtca
     6661 ggacgatttc gttgcggccg gtatcgggcg cggtctcgag cggtatggtc aacttcacgg
     6721 tcttgccgcc gcgctgcaca tcgacctgcg acgtgccgcc gagcggatgc gtcgcaaagc
     6781 ggtagtcgaa cccattggga tcgtccaccg cctgtccgtc gatcgcaacg atcagatcgg
     6841 atgccttcag cccggaccgc gctgccgggc tgctcgcaac cacactggcg accagcgcgc
     6901 cggtcggaag ccgcagcccc aggctctcgg caatctccgg cgtcaccgcc tgcaaccgcg
     6961 cgccaagcca gggccgcttg acggccttgc cgccgctctt ggcggaggcg acgacgacgc
     7021 gcaccatgtt ggccggaatg gcaaagccga tgccctgcga cccgcctgaa cgcgagaaga
     7081 tcgcggtatt gatgccggcc agccggccgg tcatgtcgac cagggcgccg cccgaattgc
     7141 cggggttgat cgcggcatcg gtctgaatga agaactggta gtcggtgata ccgacctgcg
     7201 tgcgcgccag cgccgaaatg atgccgtggg tcaccgtctg tccgacgcca aagggattgc
     7261 cgatcgccag caccatgtcg ccgaccagca attcgtcgga attggcaaag tcgagcgtcg
     7321 ggaatttctc ttgcactcct ttgagccgca ataccgccag atcgctgcga ggatccttga
     7381 gaacgatctc ggcttcgaat tctcgcttat ccgacagcga aaccttgacc tcatcggcgc
     7441 cttcgatgac atggacgttg gtcacgacga gaccggacgg atccaccatc acgcccgagc
     7501 ccagcgagcg ctgcatctgt tccggctgct ggcccggcac gccaaagaaa cggcggaaga
     7561 tcggatcgtc gagcagcgga ttacggttct gcaccatctt ggcggcatag acgttgacca
     7621 ccgccggctg cacgcgctgc acgatcggcg cgtaggaaag ctttacctcg gccggcgacg
     7681 ccgggacgcg ccggtcctgg gcgatggcac agggaagact tcccaacagg gttacggccc
     7741 atattgcggg aagcacggcg gtgcggacaa atcgatgcat tcctacctct ctgagagaac
     7801 gctgaaatat aggcgtgttc gtccggcaat agaagggtac ttggcctgtt atcctggatc
     7861 ggggacggct gccgaagcgg cgtcgccggc aaggccgcat ccccattacg tagatcgccg
     7921 gacttgcatg acgatcgtca tacgataaga tcctgttgca cccaggcaac aagggaaccg
     7981 gcaatttcgg ttaccttgtt gttagacggt caactatatt ttctacgatt ctcgcgggtg
     8041 aacgggacaa tcaattcagc gaaaggggtc atcacatgac tacgccaaga ctctcaactt
     8101 cagtagcgct cagcctggtc ggagcattga tggcatcgca ggcacacgca cagtccaccg
     8161 gcagcagtga cgcggagatt gcgttgctga aacaacagct gcgattgatg gagcaaaagc
     8221 tcgacaatct gcagaagcag accacagcga atacggcagc cgcggcgaaa gccaatgcca
     8281 aggccgatgc caaggtcacc gtcgcgaacg ccaatgcggc ctatccggtc aagggtccgg
     8341 gtgcgccatc ggatgtggtt gtgaaaatgc cgaacaaccg gccgaccatc tgcaccgcgg
     8401 atgatcagaa ttgcatttcg atcaccagcc gcgtgcattt cgacgtcggc ggttatgatt
     8461 atcatcccaa tagcgcggca accgttccgc agcgcctcga cgacggggtc aatgttcgcc
     8521 gcgcgcgtat cggcgttctc ggcaaattcc tcggcgactg gaattacgcg ctgatctacg
     8581 acttcggcgg tgcttcggac ggatttgcca gcaccgccag cgttggcgga acggcggtcg
     8641 gattccttcc gggtggcgcc ttgtcgggca ttgagaacgc ctatctgagc tacacgggct
     8701 tcaagccgtt cggtggcaag ctcgcgatcg aagggggcgt catggacctg ccctacacgc
     8761 tggacgaggc gtccagttcg aacgacatcc cgttcatgga gcgcgcctct tcaggtatta
     8821 ccgccaccaa cattgcagcc ggcgacttcc gttcggcgtt cggcgcgcgc tggtataccg
     8881 acacgttctg ggccggagcc tatgtgacgg gccccgcgac gggggctatt cactctgcct
     8941 caagcttaaa tccaaacggc accaccgaac aatacggcac cgtcgcccgt gccgcgggtc
     9001 aaatcgtcag cggcaaggat tactcactgc atatcggcgg cgaggccgaa tggctgctcc
     9061 agccaccgca caatctgatc gcggggacgc aaacgctcac cctgagcgat cgaccggaag
     9121 tgcgcatcga tccgaccacg ctggtctcga cgggcgcgat cgcgggcgta tccggcgctc
     9181 aagtctacag cgccgaagtg gcggggactt acggaccgct gttctttcaa ggagagtatt
     9241 tctggtacaa tgtcgatcgc aacgctttcg ccccgctgcc aagcctgaaa ttccagggcg
     9301 gctatgcgga ggctgcctac gtcctgaccg gagaatcccg tccctacaat ccgggcagcg
     9361 cctcctacgg cggcatcgcg ccggccaatc cgttctcatt gagcggcgac ggctggggcg
     9421 catgggagat cgccggccga tacagcacga tcgacctcaa cgatcagatc gccaccgcga
     9481 gcggcgtcgc tggcgggaga cagacgatct atacgctggc actgaactgg tatctcaatc
     9541 gcaatgtccg cctgatgttc gattaccttc atggcgatgt tgccaagcag gtgagcccca
     9601 ccaacttcgc cgataccggc tcgaagttca atgcgttcgc catgcgtacc caggtggcgt
     9661 tctaagactg gtgcttccgc tgggagcagc cgcggctgct cccggttcgt attgcgggtc
     9721 tcaggcttgc ccgcctcggc acaatcgcgc gataagaaaa ctctggaccg cgcggccccg
     9781 atcaggcggc gcgatcgggg acgtcagaca ccggcgcgca ggatagcttc tccctgtggc
     9841 cttcgcccca cgccttcaag gtatcgatca ccggacgcag gctttcgccg atttccgaga
     9901 ggcgatattc aacccgcggc ggcacttcag catagacttt gcggatcacc agatggtcat
     9961 cctccagcgc acggagctgc ttggtcagca tgcgctgcgt gatgcctggc atccgccgcc
    10021 gcaactcgcc aaagcgctgg gtgccgtcct gcaaatggta gaggatgaca cccttccact
    10081 tgccatcgat cagatcgagc gtcgcttcaa ccgcgcagcc cggccggtgg gcaaaattcc
    10141 gtcgtttcat ggggtatttt ccaatagtat ccaaacaggg actatatccc cgaatttaca
    10201 gtacttgcca atatcgcgct actcctacaa ttaggcggat gacgatttga gccaacacat
    10261 aggagacaac gggcatgaaa gccgtcggat acaaaaagtc gcttcccgtc gacgaggcgg
    10321 actcgctgat cgatttcgag acggcaaaac ccgagccgaa gggtcgcgat atcagggtcg
    10381 ccgtgaaggc gatttcagcc aatccggtcg actacaaggt gcgcaagcgc gcagcacccc
    10441 cggaaggtga gaccaagatt ttaggctacg acgccgccgg catcgtcgac gccgtcggac
    10501 cggatgtgac gctgttcaag gccggtgacg aggttttcta tgccggctcg atccagcgcc
    10561 agggcaccaa ttcggaattt catctggtgg atgagcgcat cgtcggccaa aagccgaaga
    10621 cgctgtcctt cgcgcaagcc gccgccctgc cgctgacctc gatcaccgcc tgggaactgc
    10681 tgttcgaccg tctcggtgcg gtgtccggca aaagcgtcga tccgcgcacg ctgctgatta
    10741 tcggcggcgc cggcggcgtc ggctcgatcc tgatccagct ggcgcggcgt ctcaccggcc
    10801 tgacggtggt ggcgacggcg tcccggccgg aatcgcaaaa atggtgcctc gatctcggcg
    10861 cgcatgccgt catcgatcat gccaagccga tgaaggagca gatcgaaaag ctgaagttgt
    10921 cgccggtggc gctggtcgcc agcctcacca acaccgacca gcactacaag gcgctggccg
    10981 acatcatcgc gccgcagggc aaatacggct tgatcgacga tcccccggag ttcaacgtca
    11041 gcgtcttcaa gggcaaggcg gtctcgatcc actgggaatc gatgtttact cgctcttcgt
    11101 tccagactgc tgacatgatt ggccagcatg cgctgctcaa cgatatcgcc gacctcatcg
    11161 acaagggcgt attgcgtacc acgctcgatc aggcctttgg gacgatcaac gcggccaact
    11221 tgaagcgcgc gcacgcgctg ctggaatccg gaaaatcgcg cggcaagatc gtgctggagg
    11281 ggtggtagga gtcacggcgc cgagaaggta ggatgggttt cgctttgctc aacccatcct
    11341 gcgggatcgg cgaaaccttt acacggccgg cacgccctcg acgaagatca ggcggcggtc
    11401 gagcgccgtc gcccccacct tgagcgcctc ggcgtattcc ggcgaggcgt accattctcg
    11461 caaacgctcc atcgagggaa actcgacaac gacgatgttt tttggcggcg gaccgccttc
    11521 ggcaatactt gcagcaccac cgcggaccag ataacgcccg ccatattgcg caatcgattt
    11581 ggctgcaatc gtccggtagg tctcgattgc agccgcatca cgcacttcga gttcggaaat
    11641 cacataggcc gacatctgac acctcacgca atggtctgga cgataccgcc ctcggcgcgt
    11701 agcgccgcgc cattggtcgc ggatgcctct tttgaagcga catagaccac catgttggcg
    11761 atctcctcga cggttgcgaa gcgctgtagc agcgaggtcg gcctaaactg ctttacgaat
    11821 tgcgacgccg cctcgtccac cgactggccg ttttgcctcg cgaggtcttt gacgaatgct
    11881 tcgacgccct ccgacatcgt cggccccggc agcaccgaat tgacggttac cgcggtaccg
    11941 cgggtcatct cggcgaggcc gcgcgatacc gcgagctgcg cggtcttgga cattccatag
    12001 tggatcattt ccttcggaat gttcagcgcg gattcggaag agatgaaaac gatgcggccc
    12061 cagttgcgct tgagcatgcc cggcaggtag gcgcgcgaca gccgcacgcc cgacatcacg
    12121 ttgacctcga agaaccggct ccagtcgtcg tcggcaatat cgaagaagcc tttgggctca
    12181 aaaatcccgg cattgttgat gaggatgtcc acatccggta gcatctcaac cagcgtgtcg
    12241 catcctgcgg ccgtcgaaac atcggcagcg acgcccgcg
//
LOCUS       DASWGM010000041         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_3548, whole
            genome shotgun sequence.
ACCESSION   DASWGM010000041 DASWGM010000000
VERSION     DASWGM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VGA15_01470"
     CDS             complement(<1..709)
                     /locus_tag="VGA15_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX9586401.1"
                     /translation="MTYTDSSPGAGQAQPSSDRSVEQATLIGTLMHLWPYIWPGDRLD
                     LKMRVVWSVVLLLVAKFATLSVPFTFKWAIDALTGADTAPVQASNWPLWLIASPLIMT
                     LSYGAIRVLMAVLTQWRDGIFARVAMHAVRKLAYLTFVHMHELSLRFHLERKTGGLTR
                     VLERGRLGIEVIVRMVILQLVPTVVEVTLLMGVLLWQFDWRYVLATMITVVVFMYYTY
                     IATEWRIEIRRKMNESDT"
     gene            931..1536
                     /locus_tag="VGA15_01475"
     CDS             931..1536
                     /locus_tag="VGA15_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX9586402.1"
                     /translation="MNKIKTVCVYCGSGPGTNHRFVEAAIALGKILADNGVSLVYGGG
                     SNGLMGAIAKSTLDHGGNVIGIIPEFLISRENAMARVQELIVTPDMHERKRMMFERSD
                     AFVALPGGIGTLEELVEQLTWQQLGRHAKPVLLANIDSFWEPLLALLTHMRATEFIRP
                     TLAVEILKAEHVEDILPRLLAAAARAPEGAEEMAADVIRQL"
     gene            complement(1548..1946)
                     /locus_tag="VGA15_01480"
     CDS             complement(1548..1946)
                     /locus_tag="VGA15_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9586403.1"
                     /translation="MIDHVSVGVSDLERAARFYELALAPLGLARLVTRPATVGFGKNY
                     PEFWINLRAGMAPVQPDSGTHICLRAKTADDVDAFHAAAVNAGGCSDGAPGLRPHDRV
                     RYYAAFVIDPDGNRIEAVTFPKDDAEVSPR"
     gene            complement(2064..>2256)
                     /locus_tag="VGA15_01485"
     CDS             complement(2064..>2256)
                     /locus_tag="VGA15_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX9586404.1"
                     /translation="LIDYRVRILNGGTEAVTRVLIESEDEEGERWTTIGVSPNIIDAS
                     FQALMDSVIYKLVKSGAPA"
BASE COUNT          461 a          696 c          681 g          418 t
ORIGIN      
        1 cggtgtcgga ctcgttcatc ttgcggcgaa tctcgatccg ccattcggtt gcgatgtagg
       61 tgtagtacat gaagaccacg acggtgatca tggtcgcgag cacgtagcgc cagtcgaatt
      121 gccacagcag cacgcccatc aacagcgtga cttcgacgac ggtcgggacc agttgcagga
      181 tcaccatccg gacaatgact tcgatgccga gccggccgcg ctccagcacg cgcgtcaggc
      241 ctccggtctt gcgctcgagg tgaaagcgca gcgacagctc atgcatgtgg acgaaggtga
      301 gataggcaag cttgcgcacc gcgtgcatcg ccacccgggc gaagatcccg tcgcgccatt
      361 gggtcagtac cgccatcagc acgcggatgg cgccgtagct caaggtcatg atcaggggcg
      421 acgcgatcag ccacaacggc cagttcgatg cctgcaccgg agcggtatcc gcaccggtca
      481 gggcgtcgat cgcccatttg aaggtgaacg gcaccgagag cgtcgcgaac ttggcgacca
      541 gcagcagcac caccgaccag accacgcgca tcttgagatc gagccggtcg ccgggccaga
      601 tgtacggcca cagatgcatc agcgtcccga tcagggtcgc ctgctcgacc gatcggtcgg
      661 aagaaggctg cgcttggccg gcacccgggg acgaatcagt gtaagtcatt gatcaagaac
      721 ctggagaatc gctgtcgcgc gggtgtttta tgttgatctg catggtgtcc gccatataga
      781 gcgtctgccc tccgcttgca ccctcacgga gcaattcttt cgggatttgc aggcacttgc
      841 ggcttaattc aactattgtc gccaattcaa tgggcttgac gcatttacgt tgcagtgcca
      901 catgctagta tgagcaagat caaacccaac atgaacaaga tcaaaaccgt ctgtgtctat
      961 tgcggctccg gccccggtac caatcaccgc tttgtagaag ctgccatcgc gctggggaag
     1021 atcctcgccg ataacggcgt gagccttgtc tatggcggcg gctcgaatgg cttgatgggc
     1081 gctatcgcca aatccacact ggatcatggc ggcaacgtca tcggcattat tccggagttt
     1141 ctgatttccc gcgaaaatgc catggcgcgc gttcaggaat tgatcgtgac gcccgacatg
     1201 catgaacgca aacggatgat gttcgaacgc tccgatgcat tcgttgccct gcccggcggt
     1261 atcggcacac tggaggaact ggtggagcag ctgacgtggc agcagcttgg ccgccacgcc
     1321 aagccggtcc tgctcgccaa catcgatagc ttctgggagc ccctgctcgc gctgctcacg
     1381 cacatgcgcg caacggaatt cattcggccg actctggcag tcgagattct gaaagcggag
     1441 cacgtcgagg atatcttgcc gcggttgctc gccgccgccg cccgcgcccc tgagggcgcc
     1501 gaggaaatgg ccgccgacgt gatacgccag ctttagccag cgaagggtta tcgcggcgac
     1561 acctctgcat cgtctttggg aaacgtcacc gcttcgatgc gattgccgtc gggatcgatg
     1621 acgaacgcgg cgtagtaacg cacgcgatcg tgcggacgca gacccggcgc gccgtcggag
     1681 cagcctccgg cattgaccgc ggccgcatgg aacgcatcga cgtcatcggc cgttttcgct
     1741 cgaagacaga tatgggtacc gctgtccggc tgaaccggcg ccatgccggc acgaagattg
     1801 atccagaact cgggatagtt cttgccgaaa ccgaccgttg ccggccgcgt caccagccgc
     1861 gccagcccga gcggcgccag cgccagctca taaaagcgcg cggcgcgttc gaggtcactg
     1921 acaccgacgg aaacatgatc gatcatctaa gatgcatccc gaaagtagaa acgtcattcc
     1981 ggggtgcgcg ctagcgcgaa cccggaatct cgagattccg gatatggtcc ggcggaccat
     2041 tccggaatga cggcgaggta acgtcacgcc ggcgcgccgg acttcaccag cttgtagatc
     2101 accgaatcca tcagcgcctg gaacgatgcg tcgatgatat tgggggagac gccgatggtg
     2161 gtccagcgct cgccctcctc gtcttcgctt tcgatcagca cccgcgtcac cgcctcggtg
     2221 ccgccattga ggatacgcac gcggtagtcg atcagc
//
LOCUS       DASWGM010000042         7312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1249564,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000042 DASWGM010000000
VERSION     DASWGM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249564"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /gene="clpS"
                     /locus_tag="VGA15_01490"
     CDS             complement(<1..324)
                     /gene="clpS"
                     /locus_tag="VGA15_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027548135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX9586405.1"
                     /translation="MSNNENRAGGTGGPATSVIAKVKPKTKRPNLYRVLILNDDYTPM
                     EFVVHILEKFFQKDVEAATKIMLHVHHHGIGECGVFTYEIAETKVTQVMDFARKHQHP
                     LQCVME"
     gene            802..954
                     /locus_tag="VGA15_01495"
     CDS             802..954
                     /locus_tag="VGA15_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586406.1"
                     /translation="MTIETPDPTSSVLPRCASGPDNFYMLTSATQMISAFNSIGTSLS
                     KLRVAK"
     gene            complement(1027..1368)
                     /locus_tag="VGA15_01500"
     CDS             complement(1027..1368)
                     /locus_tag="VGA15_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX9586407.1"
                     /translation="MVKVEDIQQYGKEHLETVVASATSLQNGFQAIASAYGDYTKKSF
                     EDTKSFVEKLSGVKSLDKAMEVQTEYAKSAYETFVAESQKIAGLYTDLAKQTFKPIEG
                     MVAKFAPAATH"
     gene            1766..3643
                     /locus_tag="VGA15_01505"
     CDS             1766..3643
                     /locus_tag="VGA15_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9586408.1"
                     /translation="MLRTTLASSRILRACIVGVVTTTAAVIFTNDAADARHSRHRHYA
                     RHHQEARESYNPAFSSIIVDGNSGATLSATNPDASRHPASLTKIMTLYLLFERLDAGK
                     MKLDTEMDVSEHASDQAPTKLGLRPGQTIAVEDAIKGLVTRSANDAAVVIAEAIAGDE
                     GDFAKLMTRKARTLGMVRTVYRNASGLPDDEQVTTARDQATLGRAIQDRFPRYYRYFS
                     TTAFNYHGHSIRNHNKLLGDVEGVDGIKTGYTRASGFNLVTSMRRGNRHLVGVVLGGR
                     SGGSRDTIMRGLLAENLEKAATKRTVAAITERNSSDANADVAEAAAASRPTQTVQVQG
                     AVRVASAEPAAPLPEHSTAPVPASRSILGAAAAAVPPPPAKTEPAPFTNGVIQTQQIS
                     AIPGSSEPMKPVKVKTVQVKAGQMKLASAAGQAQPVAPVANAIPAARQDVPETSSAMV
                     AKTEANKVEVAKSEPVKPEQTKPEMPPQPAGHGTGNGILGVLPASSLAPSSTPQASTS
                     QVSTSQVLAYADPAANGQPQPQTIQQNGAIKPVATHTGWIIQVGALDSETEAQERIEA
                     ARSQAKGLLTKADPFTEPVVGKGDKKLFRARFAGLDRDQAEAACRTLKRSDISCITIK
                     N"
     gene            complement(3869..4600)
                     /locus_tag="VGA15_01510"
     CDS             complement(3869..4600)
                     /locus_tag="VGA15_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9586409.1"
                     /translation="MPTLIAGIVAIILLYALLQMFRAANPVVLARAIKIGGGVVALAV
                     AAFTGIKGELAVAVPLGIFGAGLLGWSPFGASGFGNIGRMFGGSGPQRSPGQTSQVRS
                     QFLDMTLEHDSGELSGQIVAGPYAGHSLGEFDLRQLTAMISGFDAESVALLESYLDRR
                     FPAWRQDAQGNATGGQSRPAASGKMTDEEAYQILGLQPGAGRDEIGRAHRALMKKLHP
                     DQGGSTYLAARVNEAKDTLLRTHHS"
     gene            4878..5903
                     /locus_tag="VGA15_01515"
     CDS             4878..5903
                     /locus_tag="VGA15_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9586410.1"
                     /translation="MSAPTSRVSFNRRTLLRAGAGMAGIIATGRAPAFAQAQPKKLVF
                     AHINAVPESAAVAFDWMAKEVNAQSKGALEMQFFGKTLIPQELEIMNAVKSGSIAMGS
                     PAGAAATVFPEMGALLVPYLVKDYASAYAMLNGRIGDKIGKQIEDNYKLKVLCYFDYG
                     FRHFWTSKKPIVEPKDLRGLKIRVQQAKIFGDTINGLGGSAVPMAYGEVVTAAKQGVI
                     DGGDLPIVNMKALKIYEVSKYASLTYHNYGPTNAVMNLDIWNGLSAEQQKLMMDLARA
                     AQEKIREMTESVDNFAKAKSELEPLGMTVVEAKVDEFRKVAQQKIWPVYKGQYGALWD
                     EIEGFKV"
     gene            5977..6474
                     /locus_tag="VGA15_01520"
     CDS             5977..6474
                     /locus_tag="VGA15_01520"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9586411.1"
                     /translation="MLRIVSAVPKFTVTALIILAIVNLLVGVILRYFVGAITNWLDVD
                     PVPFTWVEEVGELSLAWLTLIGAAIGIQSRSHFALAVFVHRLPLTGQLWIHRFNHALI
                     SGVGLLAAWYGFKLCLLNRTLTTPGLEINLAWLYASSVVGGILIAVYGFAMMISPLPQ
                     GEPQH"
     gene            6498..>7312
                     /locus_tag="VGA15_01525"
     CDS             6498..>7312
                     /locus_tag="VGA15_01525"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9586412.1"
                     /translation="MLLATVAIVFVALILLSMPIVFALGVAGIAGLWIGGYPMQQVSS
                     ALVSGSQSWVLLAIPAFVFAGNLMERCGMSHALVELARALIGWVKGGLGMSVIVVAYF
                     FSDICGSKMAEVSALGSALMPPMTRAGYDRRDSASLIAAGTAMGMLVPPAIFMIVIAQ
                     VTNTSAVALFVAGFIPAVVIMLCLMAVVYIRARQNDWPVDSRPSLERLGHAGLHAAVP
                     MVVPFVILAGFILGIITATEAGAVVAGYAFLAAKLYYRNVSWREMGKIAYDSA"
BASE COUNT         1410 a         2324 c         2178 g         1400 t
ORIGIN      
        1 ttccatcacg cattgcaggg gatgttggtg cttgcgcgca aaatccatca cttgcgtcac
       61 cttggtctcg gcgatctcgt aggtgaacac tccgcactcc ccgatgccgt gatgatggac
      121 gtggagcatg atcttggtcg cggcttcgac atccttctga aagaacttct ccagaatgtg
      181 aacgacaaac tccattggcg tgtagtcgtc gttcaggatc aagacacggt agagattcgg
      241 acgctttgtc ttgggcttga ccttggcgat gacggatgtg gccggcccgc cggttccccc
      301 agcgcggttc tcgttgttgc tcattcgggg agcaaatgca gcgatggcgg atggcccgga
      361 cgaatcaaat ttgaaagtta gctgcaacat ggcacaggcg ttcgaaatcc ccacaggaaa
      421 ccggacagcg accaagggat ctctcaagcg atgtcctcct gccggagcca tctgaagagt
      481 tcccagacac gctttccaga tcgccgctcc cggccggccc gaccccacca accggatcga
      541 taggttggaa tatgggtccg tctccggctc gccgcaagcg cgcatatttt ggtccgatgc
      601 ggctggcggc aggccctgtt cccggctcgg cgatctgcct gaggttaatc aatcctgtcg
      661 gatttgacaa agcaaagccg ccaaccgttt ggcggacgtt gacgacagaa ggtgcctttg
      721 gggctcgatt cgcggcttac accgctattg accgagtttt ctcaaggcaa gtctaccagc
      781 tgtttggacg cgagggcggg aatgaccata gaaaccccgg atccgacgtc gtcggtgttg
      841 cctcggtgtg cgagcggtcc tgacaacttc tacatgctga cgtcggcgac ccagatgatc
      901 tccgccttca actcgatcgg aacgtcgctg tcgaagttgc gcgtcgcgaa gtaatcccgg
      961 caaattcgaa caagaaaaaa gcccggccaa acggccgggc tttttgattt cgaaaacagc
     1021 gtttgtttag tgggtggcgg ccggggcgaa cttcgcgacc atgccttcga tcggcttgaa
     1081 ggtctgcttg gcgaggtcgg tataaagccc tgcgatcttc tgcgactccg ctacgaaggt
     1141 ctcgtaggct gacttcgcgt attcggtctg cacttccatc gccttgtcca gggacttcac
     1201 gccggacagc ttctcgacaa atgacttggt gtcttcaaac gacttcttgg tgtagtcgcc
     1261 ataggcgctt gcgatggcct gaaagccgtt ctgaaggctg gttgcggagg caacgacggt
     1321 ttcgagatgc tcttttccgt actgctgaat gtcttcaact ttgaccatca tggtgtccct
     1381 tcccggattg atgtgtactt ggtgtgccgg gaggtgctgg ctccctgaca ctcgccacat
     1441 agtgcgatgc acaataaatg tcaagaaatt tgtgcgacgc acaaatatcg ggggttggtg
     1501 tcaaataccg ctatctcccg caagggttga ttaatctttt ggaaactcca gccgcctaac
     1561 ctgatccccg aacttgctcg cgctccggac aaacggccta aaattgtttg tgagcagcat
     1621 cttagcttgg atggcggcct gattggccat ccgctcggaa ttcgataccc tgatcgggcg
     1681 cggcgagcag gcaaacgcgc cggccgtcgg gcataatttg gcctcacgaa ccggtgataa
     1741 aatagagatt gggacgggga agtttatgct tcgcacaacc ttggcttcct cgcggattct
     1801 gcgggcttgc attgtcgggg tggttacgac caccgctgcg gtcattttca caaacgacgc
     1861 tgctgatgcg cgccactccc ggcaccgaca ttatgcgcgc caccaccagg aagcgcggga
     1921 gagttacaac ccggcgttct cctccatcat cgtagacggc aactccggcg ccacgctctc
     1981 tgccaccaat cccgacgcca gccgccatcc tgcttcgctc accaagatca tgacgctcta
     2041 tctgctgttc gagcgcctcg atgccggcaa gatgaagctc gacaccgaaa tggacgtgtc
     2101 cgagcacgcc tcggaccagg ctcccaccaa actcggtctg cgcccgggcc aaaccatcgc
     2161 tgtcgaagac gccatcaagg gactggtgac ccgatccgcc aacgacgctg ccgtcgtgat
     2221 tgccgaagcc atcgccggcg acgaaggcga tttcgccaag ctgatgaccc gcaaggcccg
     2281 tacgctcggc atggtcagga cggtttatcg caacgcctcc ggcctgcccg acgacgaaca
     2341 ggtgacgacg gcgcgggatc aagcgacgct gggccgcgcc atccaggacc ggttcccgcg
     2401 ctattaccgc tatttctcga ccacggcttt caattaccac ggccattcga tccgcaacca
     2461 caacaaactg ctgggtgatg tcgaaggcgt cgatggcatc aagaccggct acacccgcgc
     2521 ttcgggtttc aatctggtga cctcgatgcg ccgcggcaac cggcatttgg tcggcgtggt
     2581 attgggaggc cgcagcggcg gctcgcgcga caccatcatg cgcggcttgc ttgcggaaaa
     2641 cctcgaaaaa gccgcgacca aacgtaccgt tgcagcaatc accgagcgca attcctcgga
     2701 cgccaatgcc gacgtcgccg aggccgcggc cgcgtcacgg ccgacccaga cggttcaagt
     2761 ccagggcgcg gttcgggtgg catcggccga gccggccgca ccgctgcctg agcattcgac
     2821 cgcaccggta ccggcgtcgc gatcaatcct tggcgccgct gcagcggccg tcccgccgcc
     2881 cccagccaag accgagcccg cgccgttcac caatggggtg attcaaactc agcagatttc
     2941 ggcgatcccc ggctcgtccg agccgatgaa gcccgtcaag gtgaagacgg tgcaggtcaa
     3001 ggccggacag atgaagctgg cctcggccgc cggccaggcc cagccggttg cgcccgtcgc
     3061 caatgcgata ccggctgcac gccaggacgt gccggagacg tcgagcgcga tggtagcgaa
     3121 gaccgaagcc aacaaggtcg aagtcgccaa atcagaaccg gtcaagcctg aacagaccaa
     3181 gcctgaaatg ccgccccagc cagccggcca cggcaccgga aatggtattc tcggggtgct
     3241 gccggcatcc agcctggcac cctcctccac cccgcaggcc tccacctcgc aagtctccac
     3301 ctcacaagtc ctggcctacg ccgatccggc cgccaatggc caaccgcagc cgcagaccat
     3361 tcaacagaac ggcgccatca agcccgtcgc gacccacacc ggctggatca ttcaggtcgg
     3421 cgcactggat agcgaaaccg aagctcagga acggatcgag gcggcgcgca gtcaggccaa
     3481 aggactgctc accaaggccg atccgtttac cgaaccggtc gtgggcaaag gcgacaaaaa
     3541 gctgttccgg gctcgcttcg ccggcctcga ccgcgatcag gccgaagcgg catgccggac
     3601 cctgaagcgt tccgacatct cctgcatcac aatcaagaac tgatcaatcg ataagggccg
     3661 ataccgccgg gacaacgagc ccggccatat cggccctttg ttgttttcac atcggcgccc
     3721 acgcgcaacc tctcgtcaag actttgcggc taaaactgca acacgaacga tcgaccacgc
     3781 cggacaacgg cgggcgagaa cagggcgtcg gccggagtag aaggcagaag cagctcgcga
     3841 ttcgtagcgt ttggtagcgt tgccggagtt agctgtgatg cgtgcgaagc aaagtatcct
     3901 tagcctcgtt tacacgggcg gcgaggtacg tcgacccccc ctgatcggga tgcagtttct
     3961 tcatgagcgc gcggtgcgcg cgcccgattt cgtcccgccc cgcccccggc tgcaggccaa
     4021 ggatctgata ggcctcctca tccgtcattt tgccactcgc cgccgggcgg ctctgccccc
     4081 ctgtcgcgtt tccctgcgcg tcctgacgcc aggcgggaaa cctgcggtcc agatagcttt
     4141 cgagcagcgc cacgctttcg gcgtcgaagc ctgagatcat cgccgtcaat tgccgcagat
     4201 caaactcgcc aagggaatgg ccggcgtatg gcccggccac gatctggcct gagagctcgc
     4261 cgctgtcgtg ttcgagcgtc atgtcgagaa actgcgagcg aacctgtgac gtctgtcccg
     4321 gcgagcgttg tgggcctgag cccccgaaca ttcggccgat attgccgaac cccgacgccc
     4381 cgaacggcga ccagcccaat agccccgcgc caaaaatccc gagcgggacg gcgaccgcga
     4441 gttcgccctt gatgccggta aaagcggcga ccgcgagcgc caccacgccg ccgccgatct
     4501 tgatcgcacg ggccagaacg acgggattgg ccgcacgaaa catctgcagc agcgcgtaga
     4561 gcaggataat ggcgacaatg ccggcgatca gggtaggcat ggatccaata tagcggggcc
     4621 ggagctaaaa agcatgccgg ttgccgctct ccttttcgga agggctgaac ccggatcaat
     4681 tggtactggc gtgttttgat cccttttgac ccgatttgat tttgacagtg gcctgtttca
     4741 agcgccgatg atcgaaccga cgccccggca ccccactggc aacccgcccg ctatgctgta
     4801 agcttgtcgc cggactggtc ggcgttcccc tgcccggccg ctagaccgca aaaaaagcca
     4861 gttttggagg accccccatg tcagctccca cgagccgcgt ctcgttcaat cgccgcacgc
     4921 ttctgcgcgc gggcgccggc atggccggca ttatcgccac tggccgcgca cccgcattcg
     4981 cgcaagccca gccgaaaaaa ctcgtattcg cgcatatcaa cgcggtcccg gaatcggcgg
     5041 cggtcgcgtt cgactggatg gcgaaggaag tgaacgccca gtcaaaaggc gcgcttgaga
     5101 tgcagttctt cggcaagacc ttgatcccgc aggagttgga gatcatgaac gcggtcaagt
     5161 ccggcagcat cgcgatgggc agcccggcgg gcgccgccgc gaccgtattt cctgaaatgg
     5221 gcgcgctgct cgtaccctat ctggtgaagg attacgcctc ggcctacgcc atgctcaacg
     5281 gacggatcgg cgacaagatc ggcaagcaga tcgaggacaa ctacaagctc aaggtgctct
     5341 gctatttcga ttacggcttc cgccatttct ggacctcaaa aaagccgatc gtcgagccga
     5401 aagatctgcg cggcctaaaa atccgcgtgc agcaggccaa gatattcggc gacaccatca
     5461 acgggctcgg cggcagcgcg gtgccgatgg cgtatggcga ggttgtcacc gccgccaagc
     5521 agggcgtgat cgacggcggc gatctgccga ttgtcaacat gaaggccctg aagatctacg
     5581 aggtgtcgaa atatgcctcg ctcacctatc acaattacgg cccgaccaac gcggtgatga
     5641 atctcgacat ctggaacggg ctgtcggcgg agcagcagaa gctgatgatg gatcttgcgc
     5701 gggccgcgca ggagaagatc cgtgagatga ccgagtcggt cgacaatttc gcgaaggcca
     5761 aatcggagtt ggagccgctc ggcatgaccg tggtggaagc caaggttgac gaattccgga
     5821 aagtcgcgca gcagaagatt tggccggtct acaagggcca gtacggcgca ttgtgggacg
     5881 agatcgaagg cttcaaggtc tgagttggca gcggggcggg agtccagtct cccgcgccct
     5941 cttccgcccc attcatcttc cggaattcca tcgcgcatgc tgcgtatagt ttcagccgtc
     6001 ccgaaattca cggtcacggc gctgatcatc ctcgccatcg tcaacctctt ggtcggcgtg
     6061 atcctgcgct atttcgtcgg cgccatcacc aactggctcg atgtcgaccc cgtgccgttc
     6121 acctgggtcg aggaagtggg cgagctctcg ctggcgtggc tgacgctgat cggcgccgcg
     6181 atcggcatcc agtcgcgctc gcatttcgca ctcgccgtgt tcgtgcatcg cttgccgctg
     6241 actgggcagc tctggatcca ccgcttcaat cacgcgctga tatcaggcgt cggcctgctc
     6301 gcggcgtggt acggcttcaa gctctgcctg ctcaaccgca cgctcacgac acccggcctc
     6361 gaaatcaatc tcgcctggct ctacgcgtct tcggtggtcg gcggcatcct gatcgccgtt
     6421 tacggttttg cgatgatgat ctccccgctg ccgcaaggcg agccccagca ttgaccaggc
     6481 actgaggcgg accattgatg ctgctcgcga ccgtcgccat cgtcttcgtt gcgctgatcc
     6541 tgttgtcgat gccgatcgtg ttcgcgctcg gcgtcgccgg aatcgcaggg ctgtggatcg
     6601 gcggctaccc gatgcaacag gtttcctcgg cgctggtgtc gggatcgcag agttgggtgc
     6661 tgctcgccat ccccgccttc gtcttcgcgg gcaatctgat ggaacgctgc ggcatgagcc
     6721 acgcgctggt ggaactggcg cgcgcgctga tcggctgggt caagggcgga ctcggcatgt
     6781 ccgtgatcgt ggtggcgtat ttcttctccg acatctgcgg atcgaaaatg gcggaagtct
     6841 cagcactcgg ctccgcgctg atgccgccga tgaccagggc cggctacgac cgccgcgatt
     6901 cagcctcgct gatcgccgcc ggcaccgcga tgggcatgct ggtgccgccg gcgatcttca
     6961 tgatcgtgat cgcgcaggtc accaatacgt cggcggtggc gctgttcgtc gccggcttca
     7021 ttccggcggt tgtcatcatg ctgtgcctga tggccgtggt ttacatccgc gcgcgccaga
     7081 acgactggcc ggtcgattcc aggccgagcc tcgagcgcct cggccacgcc gggctgcatg
     7141 ccgccgtgcc gatggtggtg ccgttcgtga tccttgccgg cttcatcctc ggcatcatca
     7201 ccgcgaccga ggccggcgcc gtggtcgcgg gttacgcctt ccttgccgcc aaactgtact
     7261 accgcaacgt ctcgtggcgc gagatgggca aaatcgccta cgacagcgcg at
//
LOCUS       DASWGM010000043         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1805258,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000043 DASWGM010000000
VERSION     DASWGM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805258"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VGA15_01530"
     CDS             <1..337
                     /locus_tag="VGA15_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586413.1"
                     /translation="EAIYLADKIFLMTNGPGAVLAEIVENPLPKDRGRIDLHRHPYYY
                     ALRNHIVDFLVSRSKTFATDMPGHDPRNVPVVRIGKPELVIASVSNALHEESKQTSWP
                     GLSRPSTSS"
     gene            439..927
                     /gene="cynS"
                     /locus_tag="VGA15_01535"
     CDS             439..927
                     /gene="cynS"
                     /locus_tag="VGA15_01535"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEX9586414.1"
                     /translation="MKRSDLTEKLLDIKREKGWSWKHICGKIGGYSEVLIVGAILGQM
                     KLTKPQAANAGELFGLSKSEVAMLNEVPMRGEGTPMPPTDPLIYRFYEMVMVNGPAWK
                     ALIEEEFGDGIMSAIDFDMALERVANPKGDRVKITMSGKFLPYKYYGASGNVPEYGFK
                     EE"
     gene            complement(1042..1365)
                     /locus_tag="VGA15_01540"
     CDS             complement(1042..1365)
                     /locus_tag="VGA15_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEX9586415.1"
                     /translation="MTTFDKREEGFEKKFALDEEQKFKAEARRDKLLGLWVAEKLGLS
                     GDAASDYAKTVVAADFAEPGDADVVRKVMGDLTAKGIAITEPQIRVKMDELMAQAIVQ
                     VKAGT"
BASE COUNT          360 a          512 c          508 g          343 t
ORIGIN      
        1 tgaggcgatc tatctcgccg acaagatttt cctgatgacc aacggtcccg gcgccgtgct
       61 ggccgagatt gtggaaaatc cgctgccgaa ggaccgcggc cggatcgatc tgcaccggca
      121 cccttattac tatgcgctgc gcaaccacat cgtggatttc ctggtcagcc gcagcaaaac
      181 cttcgcaacc gatatgcccg gtcacgatcc gcgcaacgtg ccggtggtgc ggatcggcaa
      241 acctgaattg gtcatcgcat ctgtgtccaa cgcactgcac gaggaatcaa aacagacgtc
      301 atggccgggc ttgtcccggc catccacgtc ttcgtaaggg cagacagaga aagacgtgga
      361 tgcccgggac atcttgcgcg aagacgcgct tcgcgcttta gcccggacat gacgagattg
      421 aagcaaggga gacgacccat gaaacgatcc gacctcaccg aaaagctgct cgacatcaag
      481 cgcgagaaag gctggagctg gaagcacatc tgcgggaaga tcggcggcta ttccgaagtc
      541 ctgatcgtcg gcgccatcct cggccagatg aagctgacaa aaccgcaagc ggcgaatgcc
      601 ggcgagctgt tcgggctgtc caaatcggaa gtagcgatgc tcaacgaggt gccgatgcgc
      661 ggcgagggca ccccgatgcc gccgaccgat cccttgatct atcgcttcta tgagatggtg
      721 atggtcaacg gtccggcctg gaaagcgctg atcgaggagg aattcggcga cggaatcatg
      781 tcggcgatcg atttcgacat ggcgttggag cgtgtcgcca atcccaaggg cgaccgcgtc
      841 aagatcacga tgtcgggcaa attcctgccg tataaatatt acggcgccag cggcaacgtg
      901 ccggaatacg gcttcaagga ggagtgagtt tgccgtcacg ctgcgatgga aacgtcattc
      961 cggggcatgc gaagcatgaa cccggaatct cgagattccg ggcctggtcc ttcagaccat
     1021 cccggaatga cgcggagacc gtcacgtccc cgccttcacc tgtacgatgg cctgcgccat
     1081 cagctcgtcc atcttcacgc ggatctgcgg ttcggtgatc gcgatgccct tggcggtgag
     1141 atcgcccatc accttgcgga ccacgtcggc atcgccgggt tctgcgaagt cggcggcgac
     1201 gacggtcttg gcatagtcgc tggcggcgtc gcctgacaat ccgagctttt ccgccaccca
     1261 caatcccagc agcttgtcgc ggcgcgcttc ggccttgaat ttctgctcct cgtcgagagc
     1321 gaacttcttc tcgaaacctt cctcgcgctt gtcgaatgtg gtcatgatca aaggttccga
     1381 tctcgatgcc gaattggtga ctggcgggcg tcgttgcggc aattcgcctc cgcgcctaga
     1441 taatggcggg aattgttaag acaacggcct aaaagccgca gccgggcggt aaaactcgcc
     1501 cccacgggac cggatcgatt gtgctcggtg ggccaatcgg ttaggttggg cgtcaggctc
     1561 ggttctcgtt ctgtttcccg ttcctggcct tcacggcagc tccgtcgtgg gtcgcatcct
     1621 agtcatcgga gcacaccaac ttcatggatt tcaacaacac acggtacatc ccaatgagcc
     1681 gtcgacgccg catttatgaa ggcaaggcca aggtacttta tga
//
LOCUS       DASWGM010000044        11260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_695192,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000044 DASWGM010000000
VERSION     DASWGM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11260
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695192"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VGA15_01545"
     CDS             complement(<1..478)
                     /locus_tag="VGA15_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586416.1"
                     /translation="MAISRRDVLLGSAGALGAATFSFPKPAIAQSEPIKIGCLAAMTG
                     PSSAPTIGFNRGVHFAADAINAAGGVKGRKLEIVMRDTQGDPTKAVNATQELISQAKV
                     HGIWGPLNSGEALATTPIMARAKIPNIHPCVVETLIDTTKFPNAFRMAPSNSQWDDA"
     gene            complement(650..1345)
                     /locus_tag="VGA15_01550"
     CDS             complement(650..1345)
                     /locus_tag="VGA15_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9586417.1"
                     /translation="MSLAAEAGGTSIEVGSETLSDVYANVEAYYSARVAKYGATPLGV
                     DWSCQATQSLRFVQLLKICDFSVPFTLNDVGCGYGALAAFVAMRHPRSKIDYLGVDLS
                     RAMISRARRRFQSSDRRFVIGKASSRVADYSVASGIMNVNVGCSRATWEDFVATALRD
                     MRSTSRRGFSVNFVTETTEAKTSAGSSVASLYRTSPRPWVRYCEHDLGCSVETIDNYG
                     MKEFTLLVRCKQI"
     gene            complement(1342..1851)
                     /locus_tag="VGA15_01555"
     CDS             complement(1342..1851)
                     /locus_tag="VGA15_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586418.1"
                     /translation="MGRMIHDSTTFDILGQLNKRFGPDEIDEMVALQKEFAIFSSQHC
                     LEDSFALLGIEPCEKSERKRWNKFLQLLKTYKSDALNLNGYDRVIKAFEDALKTVTHF
                     PPIPPLPVFVGVHSMAEDDRVTVTEGRPLIFSVQPYVILSIPTKPGRVARQEAADAAK
                     KRRAKKTKK"
     gene            complement(1905..5135)
                     /locus_tag="VGA15_01560"
     CDS             complement(1905..5135)
                     /locus_tag="VGA15_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9586419.1"
                     /translation="MQCLRCDSQNKSGNDYCESCGAALGIKCDACNNVNGPTSHFCGQ
                     CGATLVLTPANRDPSPQRLLRSLSTKGGERKRLTVLFADIRNSTSLIDSLGDPELAMQ
                     RLEPVLNLMKDAVHRYDGIVNKIQGDGVMALFGAPRPHEDHAVRGCLAALAMQDAVAR
                     LADVSLQIRVGLHTGEVVVQTLENSIYQTYDAAGANVHLAHRMEQMADEGCILITRDT
                     YVGAKQFVEVIPLGEQTIRGIAAPVETFRLTGLLNAPASDVFRSGRRLTPMIGRNDQL
                     AALELELVNTFKGDGRVVGVVGEAGIGKSRLCFEFAEACRRQGIRVYEGRVLAHARAT
                     PFQPVLELLRDVFGIRVKDPVEVSRRRVIDRFASIAFSEQALLLLLEFLGLGDPDHPA
                     PRLDPRALKIQLLDVVRTLVRSRPGDAATVVLIEDLHWIDAASEEFVEALADAVVGTA
                     TLLVVNFRTGFVASFMQRSHYRQIFMPPLAATEAQQLLREHFGDDASLALLSRNIVER
                     AQGNPFFLEELVNTIAERGDFVGERGAYRLKGGIDKIPLPATVQAVVAARIDHLEEMA
                     KQVLETAAVIGRLVAMSILKPVSALPNDELLEAISQLRQAEMLYDLPPYDKGLLAFRH
                     PLIQEVAYAMQLRSRLIVLHAAIAKVLESFDWGKLDEFAGLLAYHYEAAGQTLEAVTH
                     LQRAAQWIGRTNSAEALKSWKRVRALLQDLPRSEHNDRLRAFSSGQILNFGWREGMSA
                     DEVKPYAEEALRYARTADRMNEPILLGAYGRILAATAAADDYVHLVQDAVKLTSGEGD
                     VGRFATVNAMLSQAFFMSGRLNEALDAAEVALAAIAEQSGFDSNVTLGLNPNQILGFD
                     VEHWIKCLRARILVRLGRFGEAEQRLAEVLQAVPERVASVVEFIPHYASVEMAWGVGN
                     PGLARPHAAKVAELAEQSGMPYLQVAAIACAGLAKATAGEFIAGARQLREAIDFARTA
                     RAGLEFEARMLADLGDALYRAGDFNAASEAVDEAIAVARRRTDRIAELHATLLRGLIL
                     AAAGDPQYVEEIGELVRQAERLLSVSGAAFFEPRLVQLRSELERRG"
     gene            complement(5465..6556)
                     /locus_tag="VGA15_01565"
     CDS             complement(5465..6556)
                     /locus_tag="VGA15_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9586420.1"
                     /translation="MKRREFLQTAGAGLAASTAVAAPAIAQSMPELKWRCTTSWPKSL
                     DVPFSASETISKYVAEATDNKFQIQAFAGGEIVPALQAVDAVSNATVEMCHTAAYYFI
                     GKDPTFALYCSVPFGLNSRQQNAWFQDHGGQDMLNEFGKKYKLYGISGGNTGTQMGGW
                     FRKEINTVDDLNGLKFRIGGWAGKTLSKLGTIPQQIAGGDIYPALEKGTIDATEWVGP
                     YDDEKLGFYKIAKYYYYPGWWEGGTALHFFINSEKWDALPKAYKSLLTIACGYANLDV
                     QARYDARNPQAIKRLVANGALLRPFSQAIMEACLKASNEVNAETSASNPDFKKIYDSQ
                     MAFRDDGNLWWQVAEYSYETFMIRTRPRG"
     gene            complement(6894..7274)
                     /locus_tag="VGA15_01570"
     CDS             complement(6894..7274)
                     /locus_tag="VGA15_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586421.1"
                     /translation="MMRGTERLSAIQIARIRSEIPRLNPDRTFMFTTAHRDLLKNPRF
                     GWPRPALLAIFPRWGGQVIPIVNFKRPFGDMTSFDIDMAAILGRPRPAPGGQFDPALW
                     RLYAEMLPALQVFVEHAQIAVGPE"
     gene            complement(7372..8790)
                     /locus_tag="VGA15_01575"
     CDS             complement(7372..8790)
                     /locus_tag="VGA15_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9586422.1"
                     /translation="MGLTITNNLKRPEPQALASTVEALAARFGNRLITSQAVRDQHAH
                     TTTWLASQPPDAVVMAQETSDIQDTVRICAKYGVPVIAFGTGTSLEGQVNAPAGGVCI
                     DLRDMSRVLEVHAEDLDCVIQPGVTRKALNEHLRDQGLFFPIDPGADASLGGMASTRA
                     SGTNAVRYGTMRDNVLALKVVRGDGEIITTGTRAKKSSAGYDLTHLFVGAEGTLGIIS
                     ELTIKLRGIPETIAAAACSFESVRGACQATILAIQTGIPVARIELLNAAQVQACNSYS
                     KLSLPETPLLLLEFHGSASEVAEQSENFSEIAKECGGGDFTWTTRPEDRTKLWQARHD
                     AYWAVKALRAGAGVVATDVCVPISRLADCVTETEADLKRLDLQSPIVGHVGDGNFHCS
                     LLCDVDDHDEMARGEDFMHRLVERAQSMGGTCTGEHGIGQGKQKYLEAELGQEAIDAM
                     RAVKQALDPQNIFNPGKILPAV"
     gene            8873..9385
                     /locus_tag="VGA15_01580"
     CDS             8873..9385
                     /locus_tag="VGA15_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9586423.1"
                     /translation="MTALTAGDDVLLRPEPFRASVMQIEPQWIDYNGHLNMAYYNVMF
                     DRAIDELWLKIGIGPGYMKERNGSTFTAECHVRYLREIHLGDPAQVSILLVAADEKRL
                     HTFKELRHASEGWLSATSENMTIHIDMAARKTAPFPPDIRARIQALANAHSTIPRPEG
                     IGRKVAMPSK"
     gene            complement(9404..9577)
                     /locus_tag="VGA15_01585"
     CDS             complement(9404..9577)
                     /locus_tag="VGA15_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX9586424.1"
                     /translation="MLSWVVTFLVIALIAGILGFGGIAGASVEIAKIIFFIAVVLFLV
                     SAVVGLARGRTRV"
     gene            9878..>11260
                     /locus_tag="VGA15_01590"
     CDS             9878..>11260
                     /locus_tag="VGA15_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX9586425.1"
                     /translation="MTEPNRLTDHGVPEHQPAAGGIAARARAATTPQYLAGLNPEQRE
                     AVETLDGPVLVLAGAGTGKTRVLTCRIAHILSTGRARPAEILSVTFTNKAAREMKLRL
                     GGMLGQAVEGMPWLGTFHSIGGRILRTHAELVQLKSNFTVLDVDDQVRLLKQLLQADN
                     IDDKRWPARMLAGLIDGWKNRGLTPSQVPPGEAAVFGNGRGGKLYAIYQERLKVLNAA
                     DFGDLLLENIRLFREHPDVLRQYQNRFKFILVDEYQDTNVAQYLWLRLLSQAPAPTSP
                     RTVFPSPLVGEGAEPRSGEAGEGPREAPLHEEAPSPGVSLRSPPPSPTRGEGKEAPSS
                     PPKNICCVGDDDQSIYGWRGAEVDNILRFDHDFPGAKVIRLERNYRSTGHILAAASHL
                     IAHNEGRLGKTLRTEDVDGEKVTVTGSWDSEEEARAIGEEIEELQRGGHKLNDIAILV
                     RASFQMREFED"
BASE COUNT         2235 a         3481 c         3347 g         2197 t
ORIGIN      
        1 cggcgtcgtc ccattggctg ttggacggcg ccatgcggaa ggcgttcggg aatttcgtgg
       61 tgtcgatcag cgtttccacc acgcaggggt gaatgttcgg tatcttggcg cgcgccatga
      121 ttggcgtagt agccagcgcc tcgcccgaat tgagcggccc ccagattccg tgaaccttgg
      181 cctggctgat cagttcctgc gtggcattca ccgccttggt cggatcgccc tgggtgtcgc
      241 gcatgacgat ctcgagcttg cggcccttga cgccgccggc ggcattgatc gcatcggctg
      301 caaagtggac gccgcggttg aaaccgatgg tcggcgccga actcgggccg gtcatcgcgg
      361 caaggcagcc gatcttgatc ggctccgatt gcgctatggc cggcttcgga aatgaaaatg
      421 tcgcagcgcc gagcgcgccc gcgctgccca ataagacgtc acgacgtgaa atggccatgt
      481 gatgctcccg ttgtttcctt ttgcgggcgc ccgtctttgc cgcgggcgtc ccggtttcgg
      541 cttttggccg aaacttggtt ttgcaattca aatttgagcc tgatgaggac gccttgtcca
      601 gagggtctgt cggacggtgg gatattgcgg gaaaacgcgc cgacttccgc taaatctgct
      661 tgcatcggac cagcagcgta aactctttca tcccatagtt atcgattgtc tcgacagaac
      721 agccaagatc gtgttcgcaa tagcgtaccc acggtcgagg gctggtgcga tagagactgg
      781 ccaccgacga acctgccgaa gttttggctt ctgtcgtctc ggtcacgaaa ttgacactga
      841 aacctcgacg gctcgtggat cgcatatccc tgagagctgt ggcgacgaag tcttcccaag
      901 tcgctcggga gcaaccgacg ttgacattca taataccgct cgcgacggaa taatccgcga
      961 cccgcgagct ggcttttcca atgacgaagc ggcggtccga actctggaaa cgccgacgag
     1021 cccggctaat catggcccgc gaaagatcta cgcccagata gtcgatcttg gatctggggt
     1081 gacgcatggc gacgaacgcg gcgagcgcgc cgtaaccgca accaacgtca ttcaatgtga
     1141 atgggaccga gaaatcacaa atcttcagaa gctggacgaa gcggaggctt tgggtggcct
     1201 ggcacgacca gtccacccca aggggcgtgg cgccgtattt ggcgaccctc gcgctataat
     1261 aggcttcgac gttcgcgtac acgtccgaca gagtttcgct gccgacctcg atgctcgtac
     1321 cgccagcttc cgccgcgaga ctcacttctt ggttttcttt gcgcgccgct tcttggcggc
     1381 gtctgcggcc tcctgccggg ccacgcgtcc gggtttggtc gggattgaaa gaatgacgta
     1441 tggctgcaca gagaaaatga gcgggcgccc ttccgtcacc gtcactcgat cgtcctccgc
     1501 catggaatgt acgccgacga acacaggcaa cggcggtatc ggaggaaaat gcgttacggt
     1561 cttgagagcg tcctcgaacg ccttgatgac tcgatcgtac ccgttcaagt taagtgcatc
     1621 cgacttgtat gtcttgagaa gctgcaggaa tttattccaa cgcttgcgtt ccgacttttc
     1681 gcacggctcg atgcccaaga gagcaaaaga atcctccagg caatgctggg aggagaagat
     1741 cgcaaactcc ttctgcaatg cgaccatctc gtctatctcg tcgggtccaa agcgcttgtt
     1801 caattgaccg agaatgtcaa acgtagtgct gtcatggatc atccttccca tcgataagcc
     1861 ctcctttgaa aatagtcact gaaatacaca acattctgcg cgcatcatcc gcgccgttca
     1921 agctctgatc gtagctggac cagccgcggc tcgaaaaacg ccgcgccgga tacactcaat
     1981 agcctctcgg cctgcctgac cagctcgcca atttcctcga catattgagg gtcgccggcg
     2041 gcggcaagta taaggccgcg caacaatgtc gcatgaagct cggcgattcg atcggttcgc
     2101 cgcctggcga cggcgatggc ctcgtcgacc gcctccgagg cggcattgaa atcgccagcg
     2161 cgatacagag cgtcgccgag atcggccaac atgcgagcct cgaattcgag ccccgcccgc
     2221 gcggttcgcg caaaatcaat tgcttcccga agctggcgcg cgccggcgat gaattcgccc
     2281 gcggtagcct ttgcaaggcc agcgcaggcg atcgccgcaa cttgcaaata gggcattcca
     2341 gattgctcgg ccaattcggc caccttcgcg gcatgcggcc gcgccaagcc tgggttgcca
     2401 actccccaag ccatctccac cgacgcataa tgaggaatga actcgacgac cgaggcgacg
     2461 cgctccggga cggcctgcag cacttcagcg agtcgttgct cggcttcgcc aaatcgaccg
     2521 agtcggacca ggatcctggc tctgaggcac ttgatccaat gttcgacatc gaacccgagg
     2581 atttgatttg gattgaggcc aagggtgaca ttgctgtcga agccgctctg ttcggcaatg
     2641 gcggcgagcg caacttcagc ggcgtcgagc gcttcgttga ggcggccgga catgaagaag
     2701 gcctggctaa gcatggcatt tacggtcgca aagcgcccca cgtctccttc gccggatgtc
     2761 agtttgaccg cgtcctgtac gagatgcacg tagtcatctg ccgccgcggt tgccgccagg
     2821 atcctgccgt acgcgccaag cagtatgggt tcgttcatcc gatccgcggt ccgcgcatat
     2881 ctgagcgctt cctcagcgta gggcttcact tcatcggcgg acatgccctc tcgccaaccg
     2941 aaattcagaa tctgaccgct tgagaaggcc cgtagccgat cattgtgctc ggatcgaggt
     3001 agatcctgca gcagcgctcg aactcttttc cagctcttga gtgcttcggc ggaattcgtc
     3061 ctgccgatcc attgggccgc gcgctgcaga tgggtgacgg cctcgagggt ttggcctgcc
     3121 gcttcatagt ggtaggcgag aaggcccgca aactcgtcaa gctttcccca gtcgaaagat
     3181 tccaggactt tcgcgatggc agcatgcaac acgattagcc gggatcgcag ttgcatcgcg
     3241 taagccacct cctggatcag cgggtgacga aacgccagaa gtcccttatc gtagggcggc
     3301 agatcgtaca gcatctccgc ttgcctgagc tgcgatatgg cttcgagcaa ttcgtcgttg
     3361 ggcaaggcgg agaccggttt cagaatcgac atagcgacca atcgcccgat cacagcggcc
     3421 gtttcgagca cttgcttggc catttcctca aggtgatcga tgcgcgcggc gacgacggcc
     3481 tggacggtcg caggaagcgg aattttgtcg atcccgcctt tcagccgata agcacctctc
     3541 tcgcccacga aatcaccgcg ttccgcgata gtgttgacaa gctcctcgag aaagaatgga
     3601 ttgccttgcg cgcgctcgac gatattccgg ctcagtaaag ccagcgaagc gtcatcgccg
     3661 aagtgttctc gcagcagttg ctgcgcctcc gtcgctgcga ggggaggcat gaagatctga
     3721 cggtaatgcg atcgttgcat gaaagacgca acgaagccgg tccggaaatt gacaacgaga
     3781 agcgtcgcgg tcccgacgac ggcatccgcc agtgcctcga cgaactcttc gctggccgcg
     3841 tcgatccagt gcagatcctc gatgagtacg acggttgcag cgtcgccagg acgtgaacgt
     3901 acgagcgtcc tcaccacgtc gagcagctgt attttcagcg ctcgcgggtc cagccgtggc
     3961 gctggatggt ctggatcccc caaacccagg aattccagca acaagagcag cgcctgctcg
     4021 gagaacgcga tcgacgcaaa ccggtctatg acgcggcgcc gcgacacctc gacggggtcc
     4081 ttgacccgaa tgccgaaaac gtcgcgcaat agttccagca ccggctggaa cggtgtagct
     4141 cgcgcgtgag ccagcacgcg cccctcgtag acgcggatgc cttgccgccg gcaggcttca
     4201 gcaaactcga aacacaggcg gctcttgccg atccctgcct cgccgacgac gccaaccacg
     4261 cggccatcgc ccttgaacgt attcaccagt tcaagttcaa gggcggctag ttgatcgttt
     4321 cgtccaatca tcggggttag acgccgccca ctgcgaaaga cgtcgctcgc cggggcatta
     4381 agcagccccg tcagcctgaa cgtttctacc ggcgccgcga tgccccgaat ggtttgctcg
     4441 ccgagcggta taacctccac gaattgtttg gcgccgacat aggtatctct ggtgataagg
     4501 atgcagccct catccgccat ctgttccatt cgatgtgcga gatggacatt cgcgccggct
     4561 gcgtcgtagg tctgataaat actgttttct aacgtttgaa cgacgacctc tccggtatgg
     4621 aggccaacgc gaatctgcaa actcacgtct gcaagcctcg cgaccgcatc ctgcatcgcc
     4681 agagcggcca gacaaccccg aacggcgtga tcctcatggg gccgcggcgc accaaagagc
     4741 gccatgacgc cgtcgccctg tatcttgttg acgataccgt cgtagcgatg aacagcatcc
     4801 ttcatcagat tgagcaccgg ctccaggcgt tgcatcgcaa gctcgggatc acccaggcta
     4861 tcgatcaagc tggtcgaatt ccggatatct gcaaaaagca ccgtaaggcg tttgcgttcg
     4921 ccgcctttag tgctcagcga ccggagaaga cgttgcggag atggatcgcg gtttgccggc
     4981 gtcaacacca gggtcgcacc gcattgccca caaaagtggc tggtcggtcc gtttacgttg
     5041 ttgcaggcgt cacacttaat tccgagagcc gcaccgcagc tttcgcaata gtcattaccg
     5101 gacttattct ggctatcgca tcgcaagcac tgcatactag ccccttcagt agcagagact
     5161 ataaccgatc aaaggtaggg tagcgatctt gctttcaatc gagatcgtga acgttgacct
     5221 gcctatcgta tccatccaca catccagtat ttgtcatttc gcctcaccgg cgggcggcca
     5281 gccggactcg cgtatttcag agcgccagcg cacgagcgag gtgatcgagt gagaggtggc
     5341 cccgacagcg ggttctggca cgagccgagc cctacagcat ctccgctctc ccgtcgctag
     5401 tcgggaaagc ggaggctgca aggacttgcc gcaaacgatg taagtgatca ctctacggtc
     5461 ctcctcaccc cctcggccgt gtgcggatca tgaaggtttc gtaggagtat tccgcgacct
     5521 gccaccagag attcccgtca tcgcggaaag ccatctgcga atcgtagatc ttcttgaagt
     5581 cggggttgga cgcggaggtc tcggcattga cctcgttcga ggccttcaaa caggcttcca
     5641 tgatcgcctg gctgaagggc cgcagcagtg ccccgttcgc gacgaggcgc ttgatcgcct
     5701 gcggattgcg cgcgtcgtat cgcgcctgga catcgaggtt ggcatagccg caggcgatcg
     5761 tgagcaagga tttataagcc ttcggcaggg catcccactt ttccgaattg atgaagaagt
     5821 gcagcgccgt gccgccctcc caccagccgg gatagtaata atatttggcg atcttgtaga
     5881 agcctagctt ctcgtcgtcg taggggccga cccattcggt ggcgtcgatg gtgccttttt
     5941 ccagcgcggg atagatatcg ccgcccgcga tctgctgcgg aatggtgccg agcttggaaa
     6001 gggtcttgcc ggcccaacca ccgatgcgga atttgaggcc gttgaggtcg tcgacggtgt
     6061 tgatctcctt gcggaaccag ccgcccatct gggtgccggt gttgccgccg gagatgccat
     6121 acaatttgta cttcttgccg aactcgttca gcatgtcctg gccgccgtga tcctgaaacc
     6181 aggcattctg ctgacgcgaa ttcagcccga acggaaccga gcaatagagc gcgaaggtcg
     6241 ggtccttgcc gatgaagtaa taggcggcgg tgtggcacat ctcgaccgtg gcattgctca
     6301 ccgcatcgac cgcctgcaag gcgggaacga tctcgccgcc ggcgaaagcc tgaatctgga
     6361 atttattgtc ggtggcttcc gcgacatatt tcgaaatcgt ctccgacgcg ctgaagggaa
     6421 cgtcgagcga cttcggccag ctcgtggtac agcgccattt cagttcgggc atcgactggg
     6481 cgatggcggg tgcggcgaca gcggttgacg cggcgagccc ggcgcctgcc gtttgcagaa
     6541 attcacgacg tttcatgatg ttttccccct tttgatttat gttttgaaac agcaatctcg
     6601 cggcgatttc gcgcgggccg ccgcccacca gggatcaggt tttcacctca accgacattc
     6661 cccggctgct ctcctggaac gccatcgacg gtaccagagg ttgaggctgc ggggtagcgt
     6721 cactcaccgg aattgaactg tctgccaagg cacctgcgtc ggcgtgccgc agcagagctg
     6781 catccctgcg gctgcacgaa tggattgtgc agcagcgtgt tctgattgtg cggaccgaac
     6841 ctgccgccgc gggcgcgcaa ggggccgcac gccacgccac gggtgggaat tccttattcc
     6901 ggaccaaccg cgatttgcgc gtgctcgacg aatacctgaa gcgccggcaa catctccgcg
     6961 taaagccgcc aaagcgcggg atcaaattgt ccaccgggtg ccgggcgcgg ccgcccgaga
     7021 atagcggcca tgtcgatgtc gaacgatgtc atgtcgccga atggccgctt gaaattgaca
     7081 atggggatga cctgaccacc ccagcgcgga aagatcgcaa gtagcgccgg acgcggccag
     7141 ccaaatctcg gatttttcag caaatcgcga tgcgctgtcg taaacatgaa agtgcggtcg
     7201 ggattgagcc gtggtatttc cgaccggatc cgggcaattt gaatggcgct cagacgttcg
     7261 gtcccacgca tcatcgcctg ttcgatcgac gcgttgtcga gatgcgcgag tggatcgaac
     7321 tccggtcgcc atgacgcgtg tcgacagcct ggccgctgtc gggtacggcc cctacaccgc
     7381 cggcaatatc tttcccggat tgaaaatatt ctgcggatcg agcgcttgtt tcaccgcgcg
     7441 catggcgtcg atcgcctcct ggccgagttc ggcctcaaga tatttctgct tgccttgccc
     7501 gatgccgtgc tcgccagtgc aggttccgcc catcgattgc gcccgctcga ccaggcgatg
     7561 catgaagtcc tcgccgcgcg ccatctcgtc gtgatcgtcg acgtcgcata acagcgagca
     7621 gtggaaattg ccgtcaccga catggccgac aatcggagac tgcagatcga ggcgcttgag
     7681 gtcggcctcg gtttcggtca cgcaatcggc cagccgcgaa atcggcacgc agacatcggt
     7741 cgccaccacg cctgcaccgg cacgcagggc cttcaccgcc caataggcgt cgtgccgcgc
     7801 ctgccagagt ttggtgcggt cctcgggcct ggtggtccag gtgaaatcgc cgccgccgca
     7861 ctcctttgca atctcgctga aattctccga ttgttcggcg acttcgctgg cgctgccgtg
     7921 aaattccagc aacagcagcg gtgtctccgg caacgacaat ttcgaatagg aattgcaggc
     7981 ctgcacctgc gccgcgttga gcagctcgat gcgcgcaacg ggaatgccgg tctggatcgc
     8041 cagaatcgtg gcctggcagg cgccgcgcac gctctcgaac gaacaggccg cggccgcaat
     8101 ggtttcggga ataccgcgca gcttgatggt gagctcggaa ataatcccga gcgtgccttc
     8161 agcgccgaca aacagatgcg tcaggtcgta accggccgag gattttttcg cgcgcgtccc
     8221 ggtggtgatg atctcgccat cgccgcgtac caccttgagc gccagcacgt tgtcgcgcat
     8281 ggtgccgtag cgcaccgcat tggtgccgga cgcccgggtc gacgccatgc cgcccagcga
     8341 cgcatcggcg ccgggatcga tcggaaagaa caatccctgg tcgcgcagat gctcgttgag
     8401 cgccttgcgg gtgacgccgg gctggatcac gcagtcgaga tcctcggcgt gaacctcgag
     8461 cacccggctc atgtcgcgca gatcgataca gacaccgcca gccggcgcgt tgacctgccc
     8521 ctccagcgag gtgccggtgc cgaacgcgat cacgggaacg ccgtatttgg cgcagatccg
     8581 caccgtgtcc tgaatatccg atgtctcctg tgccatgacg accgcgtcgg gcggctgcga
     8641 tgccagccat gtggtggtat gcgcgtgctg gtcgcggacc gcctgcgagg tgatcagccg
     8701 gttgccgaac cgcgcggcga gcgcttcgac ggtgctcgcc agcgcctgcg gctccggtcg
     8761 cttcaaattg ttggtgatgg tgagacccac gcgatatcct cccggcgcaa gaccgtggca
     8821 aaggatatat aaagggtcaa gggatcatca accgaaccga cagggatcaa cgatgacggc
     8881 gctcactgcc ggcgacgatg tgctactccg gcctgaaccg tttcgggcct cggtgatgca
     8941 gatcgagccg caatggatcg actacaatgg ccatctcaac atggcttact acaatgtgat
     9001 gttcgaccgc gccatcgacg aattatggct caagatcggg atcggacccg gatacatgaa
     9061 ggagcgcaac ggctcgactt tcaccgccga atgccacgtg cggtacttgc gcgaaattca
     9121 tctcggcgat cccgcgcagg tttcgatcct gctggtcgcc gccgatgaaa agcggctgca
     9181 taccttcaag gaattgcgcc atgccagcga aggctggctg tccgcaacct cggaaaacat
     9241 gactattcat atcgacatgg ccgcgcgcaa gaccgcgccg tttccgcctg atattcgcgc
     9301 ccgcatccag gctttggcga acgcccattc gaccatcccg cggcccgagg gcatcggccg
     9361 aaaagtcgcg atgccctcga aatagatctg atccgcgggc tcgtcagacg cgggtccgcc
     9421 cgcgcgccag accgaccacc gccgagacca gaaacagcac caccgcgatg aagaagatga
     9481 tcttggcgat ttcgacggac gctccggcaa tcccgccaaa acccagaatg cccgcgatca
     9541 gcgcgatgac caaaaacgta acgacccagc tcagcatgat accaacctct gttttgcatc
     9601 ttgtgtttca gaaccagcgc gacggagagc gtcgcgcatc ggccgcggaa ataatccgca
     9661 tttgcaacag atgttccgcg taggtccggg cgctgcgaac tgaaaaatgc tgattcccag
     9721 ggaacaaaat ggaggctgga tggcgaaatt cgccctgcgg gcgcggacgg gataaacctg
     9781 tcaaatccac tgccaagccc ctatatgccc ttcaatcctg ctatgaaggc tccccttcaa
     9841 ggccccacgg tgccatcgtg ggcggatgac gattcgcatg actgagccga acagactgac
     9901 cgatcacggc gttcccgagc atcagcctgc ggctggtggc attgccgcgc gcgcccgcgc
     9961 ggccacgacc ccgcaatatc ttgccgggct caatcccgag cagcgcgagg cggtggaaac
    10021 gctggacggt ccggttttgg tgctggcggg cgccggcacc ggcaaaaccc gggtgctgac
    10081 ctgtcgcatc gcccacattc tcagcacggg ccgcgcccgc cccgccgaaa tcctgtcggt
    10141 caccttcacc aataaggcgg cgcgcgagat gaaactgcgg cttggcggga tgctcggcca
    10201 ggccgtcgaa ggcatgccgt ggctcggcac ctttcattcg atcggcgggc gaatcctgcg
    10261 cacgcacgcc gaattggtgc agctgaaatc caatttcacc gtgctcgatg tcgacgacca
    10321 ggttcgtctg ctgaaacagc tcctgcaggc ggacaacatc gacgacaagc gctggccggc
    10381 gcgcatgctg gctggattaa tcgacggctg gaagaatcgc gggctgacgc cgtcgcaggt
    10441 gccgccgggc gaggccgcgg tattcggcaa cggccgcggc ggcaagctct acgccatcta
    10501 tcaggaacgg ctaaaagtcc tcaacgccgc cgacttcggc gatctgctgc tggaaaacat
    10561 ccggctgttt cgcgaacatc cggatgtgct ccggcagtac cagaaccgat tcaagttcat
    10621 tctggtggac gaatatcagg acaccaacgt ggcgcagtat ctgtggctgc ggctgttgtc
    10681 gcaggcgccg gcgccgacct cgccgcgcac cgtcttcccc tctccccttg tgggagaggg
    10741 tgccgaaccg cgaagcggtg aggcgggtga ggggccgcgc gaagcgcccc ttcatgaaga
    10801 ggccccctca cccggcgtct ctctgcgctc gccgccaccc tctcccacaa ggggagaggg
    10861 gaaagaggca ccctcctcgc cgcccaaaaa catctgctgc gtcggcgatg acgaccagtc
    10921 gatctatggc tggcgtggtg ccgaggtcga caatatcctg cgcttcgacc atgattttcc
    10981 cggcgcgaaa gtcattcggc tggagcgcaa ctaccgctcc accggtcata tcctggcggc
    11041 cgcctcgcat ctgatcgcgc ataatgaggg ccggctcggc aagacgctgc gcaccgaaga
    11101 cgtcgacggc gagaaggtca ccgtcaccgg ctcctgggat tccgaagagg aagcccgcgc
    11161 catcggcgag gagatcgagg agctgcagcg cggcgggcac aagctcaacg acattgcgat
    11221 cctggtgcgg gcgtcgttcc agatgcgcga attcgaagat
//
LOCUS       DASWGM010000045         3220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1388068,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000045 DASWGM010000000
VERSION     DASWGM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388068"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..1808)
                     /gene="dnaA"
                     /locus_tag="VGA15_01595"
     CDS             complement(378..1808)
                     /gene="dnaA"
                     /locus_tag="VGA15_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX9586426.1"
                     /translation="MTNIEQDRWSRVKGRLRSSVGEDVYSSWFARMDLESVQDESVRL
                     SVPTRFLKSWIQAHYAERVLTCWQAEMPEVHRIDLTVRTAMRCAAPAREALAPLDQRR
                     TERPDGRPAPELRATAIAPVSASHDALGGSPLDPRLTFGSFVIGRSNTLAHAAARQVA
                     EGRRGDSVMFNPLYIHAGVGLGKTHLLQAVTWAGNSGSERKVLYLTAEKFMYGFVAAL
                     KTQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPS
                     DLESLDDRVRSRLAGGLVVEMGSLGEELRLGILKSRVAAARAHHASFDVPAPVLDYLA
                     RTITHNGRDLEGAINRLLAHSKLNAQPVTLEMAEREVRDLIRPQEPKRIKIEDIQRVV
                     ARQYNVSRSDLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAV
                     RKIEALVTRDVALSEEVESLKRQLQE"
     gene            complement(3056..>3220)
                     /gene="rpsT"
                     /locus_tag="VGA15_01600"
     CDS             complement(3056..>3220)
                     /gene="rpsT"
                     /locus_tag="VGA15_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9586427.1"
                     /translation="VEEAIKSGDRDAALKAMTRAEPELMQAAQRNIIHKNNASRKVSR
                     LAHQIAKLAK"
BASE COUNT          624 a          974 c          961 g          661 t
ORIGIN      
        1 gttgcgcgcg ttcgacggta accttcattg caatacccgc ctgatcctaa gatggaaaag
       61 ccgggcggcc cggaaggcgc gccgcggcgt ttgaaacccg aaaagccgat catagctgga
      121 tttgatcaaa cccgcagggg gaccgcaagg tggcgcggat ggcgcgcaag cgcaagggat
      181 gcgttaggca gttccccacg ttttacggga aatccggcct ttgcgggtcg tcccggcgaa
      241 atgccgggac ccataacccc tggcgtcaat ggccgcagaa ggcatccgcc ccactgccga
      301 aacgagatgt cacggcgtat ggatcccggc gttcgccggg acgaccaatc cgagacggcg
      361 ccttgttaca gagacgctca ctcctgcaat tgacgtttca gcgattcgac ctcttcggag
      421 agcgccacat cccttgtgac cagggcctcg atcttgcgca cggcgtgcag gaccgtggtg
      481 tggtcgcgcc ctccgaaccg gcggccgatt tcgggcagcg agcgcagcgt cagcgtcttg
      541 gccagataca tcgcgacctg gcgtggccga acgacattcg ccgtacgccg cgacgacagc
      601 aggtcggaac ggctgacatt gtattgccgg gccaccaccc gctgaatgtc ctcgatcttg
      661 atgcgtttgg gctcctgcgg acggatcagg tcgcgcactt cgcgttcggc catttccagt
      721 gtcaccggct gcgcgttgag cttgctgtgc gcgagcaggc ggttgatggc gccttcaagg
      781 tcgcggccat tgtgggtgat ggttctggcc agataatcca gcaccggtgc cggcacgtcg
      841 aagctcgcat gatgcgcccg cgcggctgcg acccgcgatt taaggattcc gagccgcaac
      901 tcctcgccga gcgaacccat ctcgaccacc aggccgcccg cgagacgcga gcgcacgcgg
      961 tcgtcgaggc tctccaggtc ggaaggcgga cgatcggcgg cgatcaccac ctggcggccg
     1021 gcatcgatca acgcattcag ggtatgacag aattcagcct gggtcgattt gccctgcagg
     1081 aattgcagat cgtcaatcac cagcacgtcg atgccgcgca acgcttcctt gaatgcgagc
     1141 gcggtctggg tcttgagcgc cgcgacgaag ccgtacatga atttttcggc cgtgaggtaa
     1201 agcaccttgc gttcgctgcc ggaattgccg gcccatgtca cggcttgcag caggtgggtt
     1261 ttgccgagcc cgacgccggc gtggatatag agcggattga acatcacact atcgccgcgc
     1321 cgcccttccg caacctggcg cgccgcggcg tgtgccagcg tgttggaacg gccgatcaca
     1381 aaacttccga atgtcaggcg cggatcgagc ggcgaaccgc cgagcgcgtc atggctggcg
     1441 gaaaccggcg cgatcgcggt cgcacgcagc tcaggcgcgg gacgaccatc gggtcgctcg
     1501 gtgcgccgtt ggtcgagcgg cgcgagggcc tctcttgccg gcgccgcgca tcgcatcgcc
     1561 gtgcgcacgg taaggtcgat ccggtgcact tccggcatct cggcctgcca gcaggtgagg
     1621 acacgctcgg cataatgcgc ctggatccag ctcttgagga accgcgtcgg aaccgaaagg
     1681 cggacgcttt cgtcctgcac gctctccaga tccattcgcg caaaccagct tgaatagacg
     1741 tcttcgccga cgcttgagcg cagccgaccc ttcacgcgtg accagcgatc ctgttcgata
     1801 tttgtcattg ccttacgact tttctgatgg agtgatattg cctgcgaaac gccgtgcggg
     1861 tgttccgcac ggcaggacct cgatcggggc ttcgtcttca gccatagacc ggccgttcgg
     1921 cgggatgccg cgggcgaaat catcaggctg gaagaagaag cgtccgcgag gtcagcgcgt
     1981 actcaacgga gtgtccggag ttcgcgtcag cggatgcgcg caggcggcat ccgaggggtt
     2041 ccattcagtg tcgtcggaaa atggtatttg atcgaatacc gcgttgattc cataaagcat
     2101 gcaacctccc catcccgcca tgattgctca cagcaagttg tcatatcacg tgagtttcaa
     2161 attcagtcga tcgcgtccaa acatcaaaat cgttcagccg cttcgccgcc gcgttggtta
     2221 gccccgttac aacatgcccg cctgcttcac cttcggcgcg atcgtggttg gctgtgcgaa
     2281 tatctcttgt tcaaagcaag cgaggcgcaa tgcccctgct tgagaaattt cgacacagaa
     2341 ctaccgtcct catattgcta tgagtttaga tccgacaatc gcttggaaag cgtcgccaac
     2401 gcgcacaccg ccgccgattt gcacgtccgc cccgttcctt aaaccgcgct ggtctctgga
     2461 gccgtaggcg tcgcgaacgt tgttagagat accttcatgc cctatttccc gcaacggaaa
     2521 tgattccagg ggcgcgccgg tagaattgac ttcgcacttg caaaaacagg cagcgatttt
     2581 cgcgcaaagg gccaaggttt tgaatctgtg cggagattcg aactgccgcg gcgctgtcac
     2641 atttctcacg gctgcccaaa ataaattcat aaatcgttta cgtttgagcg cggcagaacg
     2701 gaattttcgg acaggtgctc gccgctatgc cgataagtga ctacgtaaac gcgttttttt
     2761 ttgctcgtcg gaacagggta accctgaggg agccttgtac accgtcgcgc gatcgtgatt
     2821 gctatttcaa aaacctgcgc gatcaataac cggcaatcaa tattgtcgcg gtcatcgcgg
     2881 gctttgtaaa cctgtctgcg tgcatacggc ggaagaatta ccgtagaaat gcgggggcag
     2941 tcagagtttc gggaagccga gacctgccaa gccgagacct gcttgccata cgccgacgaa
     3001 aaagaagaag cccggccatg ccgggcttcc ctgacgaata ttttcgttga tcggttcatt
     3061 tcgctagctt tgcgatctga tgcgcgagcc gcgagacctt tcggctcgca ttgttcttgt
     3121 gaatgatgtt gcgctgcgct gcctgcatga gttccggttc cgcacgcgtc atcgccttta
     3181 gcgccgcatc gcggtcgccg ctcttgatcg cctcttcgac
//
LOCUS       DASWGM010000046         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2359751,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000046 DASWGM010000000
VERSION     DASWGM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2359751"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..789
                     /locus_tag="VGA15_01605"
     CDS             31..789
                     /locus_tag="VGA15_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9586428.1"
                     /translation="MKKFLLATVGLVALGMAAPASAADLAARPYTKAPPPMIAAIYDW
                     SGFYIGANGGWGQERRCFDAVTVAGTFLGSEGCHTASGGVAGGQIGYRWQTGGWVFGL
                     EAQGDWANLRGDNVSLFFPGFVNRSKLDAFGLFTGQVGYAWNNALFYVKGGAAVVDDR
                     NDILFAGAVVGSTRTDTRWGGTVGVGFEYGFAPNWSAAIEYDHLFLDNRTATFTTPAG
                     AFFAADRIRGDADLVTARINYRFGWGGGPTIAKY"
     gene            complement(1000..>2365)
                     /locus_tag="VGA15_01610"
     CDS             complement(1000..>2365)
                     /locus_tag="VGA15_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9586429.1"
                     /translation="HNIPEWIMVEFGAAMAGVILVTVNPGLRSGEVEYVLKQSRAAGV
                     MVVPEFRGNSMLATVQALASRLPELREIICFDQWDAFLASGDAAKPELPTVRSTDAVM
                     IQYTSGTTGFPKGALLHHRGLVNNGAHTADRAGIPDGAVQVTTMPLFHTGGCVCCVLG
                     AVSKRATQVLVEAFDPGLVIELMATYRSNAMLGVPTMLVAMMEHPSFASADLSSLKAI
                     YSGGSTVPAAIVADLEKRLGAPFTIVFGQTECSPVALMTWATDSIADKANTIGTPMPN
                     VEVKIANPDTGQTVPIGEIGEICTRGYHVMQGYFEDEAATRAAIDPEGWLHTGDLGAM
                     DARGYCTIEGRLKDMIIRGGENIYPRELEELLFRHPKVGDVAVVGLPDQRWGEVVAAF
                     IRPPPGIVVDKDELHDYAREHLAPHKTPRHWFVVDTMPLTGSGKIQKFKLREQWSKGE
                     MKEL"
BASE COUNT          394 a          747 c          745 g          479 t
ORIGIN      
        1 cactgcgggc gacggggatg gagaaatacg atgaagaaat ttttgttggc cacggttggt
       61 ctggtagcgt taggtatggc cgctcccgcg tcggcggctg atctggcggc acgtccttac
      121 accaaggcgc cgcccccgat gatagctgcg atttacgact ggtcaggttt ttacatcggc
      181 gcgaacggcg gttggggcca ggagcgccga tgcttcgacg cagtaactgt agccggcacg
      241 tttcttggca gtgagggctg ccacactgcg tcgggcggcg tcgccggtgg ccagatcggc
      301 tatcgctggc agaccggtgg ctgggtattc ggcctcgaag cacagggcga ctgggccaat
      361 cttcgcggcg acaacgtgag cctctttttc cccggcttcg ttaatcggtc caagttggac
      421 gcattcggcc tattcaccgg ccaggtaggc tacgcttgga acaacgctct gttctacgtt
      481 aagggcggcg ccgcggtcgt cgacgaccgc aacgacatcc tctttgctgg agccgttgtt
      541 ggatcgactc gcaccgacac tcgttggggt ggcacggttg gtgtcggttt cgaatatggc
      601 tttgctccga attggtcggc cgcgattgag tacgaccatt tgttcctcga taaccgcacg
      661 gcgacattca cgacacccgc cggcgcgttc tttgccgccg accgcattcg cggcgatgcc
      721 gaccttgtca cggcccgaat caactatcgc ttcggctggg gcggaggccc gacgatcgcg
      781 aagtattgat ctgatctgtc tgatctatct ccagcaaact caagccccgg catcgtccgg
      841 ggcttttttg gccgctacct ctccgacgcc tgctgtgggg tcgttcgcgt cggtttggtg
      901 atgtccggga tatggccggt tagcgggtag tctcggaagt gtcgattgtc cggttttgcc
      961 gcgatggcct gaggccgccg cttgccagcc gcgcccgtct cacagctctt tcatctcgcc
     1021 tttcgaccat tgctcgcgca gcttgaattt ctggatcttg ccggatccgg tgagcggcat
     1081 ggtgtcgacc acgaaccaat ggcgcggggt cttgtgcggg gcgagatgct cgcgcgcata
     1141 gtcatgcagt tcgtccttgt cgacgacgat gcccggcggc ggccggataa acgccgcgac
     1201 gacttcgccc catctttgat ccggcaatcc gaccaccgcg acgtctccga ccttgggatg
     1261 gcggaacaac aattcctcca gctcgcgcgg ataaatgttt tcgccgccgc gaatgatcat
     1321 gtccttcagc cgtccctcga tcgtgcaata gccgcgcgca tccatcgcgc cgaggtcgcc
     1381 ggtgtgcagc cagccctccg ggtcgatcgc cgccctggtg gccgcttcgt cctcgaagta
     1441 accctgcatg acatgataac cgcgcgtgca gatctcgccg atctcgccga tcggaacggt
     1501 ctggccggta tccgggttgg cgatcttcac ctcgacgttc ggcatcggcg tgccgatggt
     1561 attggccttg tcggcgatgc tgtcggtggc ccaggtcatc agggcgaccg gcgagcactc
     1621 ggtctggccg aacacgatcg tgaagggcgc gcccagccgt ttttcgagat ccgccacgat
     1681 cgccgccggc accgtcgagc cgccggaata gatcgccttg agcgaggaaa gatcggctga
     1741 cgcgaacgac ggatgctcca tcatcgcgac cagcatcgtc ggcacgccaa gcatcgcatt
     1801 gctgcgatac gtggccatca gctcgatcac gagcccagga tcgaacgctt cgaccagcac
     1861 ctgggtggcg cgcttcgaca ccgcgccgag cacgcaacag acgcagcctc cggtatggaa
     1921 cagcggcatg gtcgtgacct ggactgcgcc atcgggaatg ccggcgcggt ccgcggtgtg
     1981 cgccccgttg ttgacgaggc cgcgatggtg cagcagcgcg cctttgggga agcccgtcgt
     2041 gcccgacgtg tactggatca tcaccgcgtc ggtcgaccgc accgtgggca attcgggctt
     2101 tgccgcgtcg cccgatgcca gaaatgcatc ccactgatcg aagcagatga tctcgcgcag
     2161 ttccgggaga cggctggcaa gcgcctgaac ggtcgccagc atcgaattgc cgcggaattc
     2221 cggcaccacc atgacgccgg ccgcgcgcga ctgcttcagg acatattcga cctcgcccga
     2281 tcggaggccg gggttgaccg tcaccagaat gacgcccgcc atggcggcgc caaactccac
     2341 catgatccac tcgggaatgt tgtgg
//
LOCUS       DASWGM010000047         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1388301,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000047 DASWGM010000000
VERSION     DASWGM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388301"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VGA15_01615"
     CDS             complement(<1..954)
                     /locus_tag="VGA15_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9586430.1"
                     /translation="MQNWVEHALWWHVYPLGFTGAESKACDRLGVLHRLGHVKAWLDY
                     AVDLGASGLLLGPIFASSTHGYDTVDHFRIDQRLGDDTDFDALIEAAHSRGLRVILDG
                     VFNHVGREFPVFQRVIAEGPAAPEAAWFHLSWPMTPRPGAEPDYATFEGHRQLVALNH
                     DEPAVADYVTRVMNCWLRRGADGWRLDAAYAVPRRFWANVLSDVRADYPDAYLFGEVI
                     HGDYAGFVRETGVDAVTQYELWKAIWSALNDRNFFELGWALDRHNSWLDAFVPLTFVG
                     NHDVTRIASQLTDERHLPHALVILLTCGGTPSIYSGDEQAFR"
     gene            complement(991..1692)
                     /locus_tag="VGA15_01620"
     CDS             complement(991..1692)
                     /locus_tag="VGA15_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586431.1"
                     /translation="MPEDKFLDIRDLDAGYGRSQVLFGVTLHAPWRGGVAILGRNGAG
                     KTTLMKAIVGELPLLRGSITLDSRDVGQLPTEQRIRSGVGYVPQEHSVFARLSVRDNL
                     AVGAMTNRDSQAVNRILEIFPKLGQRMDQIAGTLSGGERKMLAIGRALLSDPRLLLLD
                     EPTEGVWIGVIEEITERLVLLAKEIAVVIVEQHLDLALEVASHAYVLDRGRVALVGSA
                     AEVRHDPRLLQYLSP"
     gene            complement(1685..2419)
                     /locus_tag="VGA15_01625"
     CDS             complement(1685..2419)
                     /locus_tag="VGA15_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586432.1"
                     /translation="MALLEARGLVKIFGKLMALNGAELTVAENEFHGLIGPNGSGKST
                     LMKCVAGAEVPTDGSVLFAGRDITSATPAERARAGMSLKFQSTSVLPALTLYDNVLLA
                     LQAQMSLSSLVFSRTRGVLHDRVMAMLEQFRLADRADSPAAALSHGQQQWLEIAMALA
                     PEPKLLLLDEPTGGMSLEERRVTGELLQPIKKKCSLVIVEHDLDFIRDICDRLTVLDQ
                     GSVLDCGSVREIQSSAKVQEVYLRRA"
     gene            complement(2406..3437)
                     /locus_tag="VGA15_01630"
     CDS             complement(2406..3437)
                     /locus_tag="VGA15_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9586433.1"
                     /translation="MADLAVSPRSRTAFGWQKLPIIELIVLLIVAVLPLVLQDYLTVY
                     ATRVLILCLFALSFDLVWGYAGIMSFGQAVFFGSAGYGVALLARDLGITSIFFVLPAG
                     VLIGFTFALLLGGFLLLGRHPSSVIFVSLGTLTGSYAVDRLARGWYYLGGQNGIPSFS
                     TMTLGSYEFEEGAVFYYLALGFLILVYLLCRFLTRSQFGLALAGLRENEQRISFFGYK
                     VQHLKAIIFSLSGAIAGLAGSLYAFHEGFVWPNMLGVVMSTQVVLYVLFGGAGTLIGA
                     VIGTVVIESVSFWLSNNYQDIWPIILGVLLLLVILFRPAGLISLVLSERERVGNFGRP
                     PKKGSDGAP"
     gene            complement(3517..>3676)
                     /locus_tag="VGA15_01635"
     CDS             complement(3517..>3676)
                     /locus_tag="VGA15_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586434.1"
                     /translation="FDHAKIAEGPGGPAEMVPGQRHCKMKMYTAVAKGGNYEIVARSA
                     GLVDPKEC"
BASE COUNT          824 a         1143 c         1077 g          632 t
ORIGIN      
        1 gcgaaacgcc tgctcgtcgc cggaatagat cgaaggcgtg ccgccgcagg tcagcagaat
       61 gacgagcgcg tgcggcaagt gcctttcgtc ggtcaactgg ctcgcgatgc gcgtgacgtc
      121 gtgattgccg acgaaggtga gcggcacgaa cgcatccagc caggaattat gccgatcaag
      181 cgcccagccg agttcaaaaa aattgcgatc attcagcgcg ctccagattg ccttccagag
      241 ctcgtattgg gtgaccgcat cgacccccgt ctcgcgaaca aagccggcat agtccccgtg
      301 gatgacctcg ccgaacaggt aagcatccgg ataatcggcc cggacatcgg acaagacatt
      361 cgcccagaac cgtctgggga cggcataggc agcgtccagc cgccaaccat cggcaccgcg
      421 ccgcaaccag cagttcatga cgcgggtgac ataatcggca accgcgggct cgtcatgatt
      481 cagggccacg agttggcgat ggccttcgaa ggtcgcgtag tccggctctg ccccaggtcg
      541 cggcgtcatc ggccacgata aatggaacca agcggcctca ggagctgccg gtccttcggc
      601 gataacgcgc tgaaagaccg gaaattcacg gccgacgtgg ttgaagacgc cgtcgagaat
      661 gacccgcaaa ccacgactat gtgcggcttc gatcaacgcg tcaaaatcgg tgtcgtcgcc
      721 gagacgttga tcaatcctaa aatggtcgac ggtgtcgtaa ccgtgcgtag acgaggcgaa
      781 aatcggcccc agcaaaagcc cggacgcgcc gaggtcgacg gcgtagtcca gccacgcttt
      841 gacatggccg aggcggtgga ggacgcccaa ccggtcgcat gccttggatt cggcgcccgt
      901 aaatccaagc ggatagacgt gccaccacag cgcgtgttcg acccaatttt gcaaagcttt
      961 tccttctgga gtcgaccaac agcaaaatat ctagggcgac aaatactgca gcagtctggg
     1021 atcgtggcga acctcggccg cagatcccac gagggcaacg cgtccgcgat cgagcacgta
     1081 agcatggctg gcgacctcta aggcgagatc gagatgctgc tcgacgatga caaccgcgat
     1141 ttcttttgcc agcaggacga gacgttcggt aatctcttcg atcaccccga tccacacgcc
     1201 ttccgtgggc tcgtcgagca ggagcagccg ggggtcggac aagagagccc ggccgatcgc
     1261 cagcatcttt ctctcaccgc ctgacagcgt gcccgcaatc tggtccatgc gctgaccgag
     1321 ttttgggaat atttcgagta tccgattgac ggcctgactg tcgcggtttg tcatcgcgcc
     1381 aaccgcgagg ttgtctcgta ccgaaagacg ggcgaatacc gaatgctcct gcgggacata
     1441 tccgacgccc gatcggatgc gctgctcggt cggcagttgt ccaacgtccc gcgagtccag
     1501 cgtgatcgat ccgcggagca gcggcagctc gccgacgatg gccttcatca gcgtggtctt
     1561 gccggcgcca ttgcggccaa ggatggcgac gccgccgcgc cagggcgcat ggagcgtcac
     1621 gccgaacagc acctggctgc ggccgtagcc ggcatcgaga tccctgatat cgagaaattt
     1681 gtcttcaggc acggcggaga taaacctcct gcaccttggc gctggattgg atctcacgga
     1741 ccgagccgca gtcgagcaca ctgccctggt cgagcaccgt caggcgatcg cagatgtcgc
     1801 gaatgaaatc gagatcatgc tcgacgatca ccagcgagca ttttttcttg atcggctgca
     1861 acagctcgcc ggtgaccctg cgctcttcca ggctcatgcc tccggtcggc tcatccagca
     1921 gcagcaattt tggttcgggg gccagcgcca tggcgatctc gagccattgt tgctgcccgt
     1981 gcgacagtgc cgcggcggga ctatccgcgc gatcggcaag ccggaattgc tcgagcatcg
     2041 ccatcaccct atcgtgcaga acgccgcggg tgcgcgaaaa caccagactg gacaatgaca
     2101 tctgcgcctg caacgccagc agaacattgt cgtagagggt caacgccgga agaacgctgg
     2161 tgctctggaa tttcaggctc atgccggcac gcgcccgttc cgccggcgta gccgacgtga
     2221 tgtcgcgccc ggcaaacaac accgagccat cggtcgggac ttccgcaccc gccacgcatt
     2281 tcatcagggt gctcttgccg gacccgttcg gtccgatcag gccatgaaat tcgttttcgg
     2341 cgaccgtgag ctcggccccg ttcaacgcca tcagcttgcc gaagatcttg accagtccac
     2401 gggcttcaag gagcgccatc gctgcccttt ttcggtggcc gaccaaagtt gccgacgcgt
     2461 tcgcgctcgc tcaagaccag actgatcagc cctgcggggc gaaacaatat cacgagcagc
     2521 agcagcacgc ccaggatgat gggccagata tcctgatagt tgttcgagag ccagaagctg
     2581 acggactcga tcacgaccgt gccgatgacg gcaccgatca gtgtccccgc cccgccaaac
     2641 agaacgtaga gcacaacctg ggtcgacatc acgacgccca gcatattggg ccagacgaag
     2701 ccttcgtgaa aggcgtaaag gctgccggca agtccggcga tggcgccgct gagtgaaaag
     2761 atgatggcct tgaggtgctg aaccttgtag ccaaagaagg agatgcgttg ttcgttttcc
     2821 cgcaggccgg caagggcaag accgaattgc gagcgcgtca ggaagcgaca caacaaatag
     2881 accaggatca gaaaccccag ggcaagataa taaaataccg cgccttcctc gaattcgtag
     2941 ctgcccagtg tcatcgtcga gaacgacgga atcccgtttt gccctccgag atagtaccat
     3001 ccgcgcgcca gacgatcgac ggcgtacgag ccggtcagcg tgcccaatga tacgaaaatg
     3061 acgctggagg gatggcgtcc cagcaacaga aagccgccga gcagaagcgc aaaggtaaat
     3121 ccgatcaaga cgccggccgg cagcacgaaa aagatcgagg taatgccgag atcccgcgcc
     3181 agcaacgcga cgccgtatcc ggccgagccg aaaaacaccg cctggccaaa gctcatgatt
     3241 cccgcgtacc cccaaacgag atcgaaggag agtgcgaaca ggcacaggat cagcacccgc
     3301 gtggcataga cggtcagata gtcctgaagc accagcggca gaaccgcgac gatcaacaga
     3361 acgatcagtt ctatgatcgg cagcttttgc catccgaaag cggtccgcga ccgaggcgag
     3421 accgccaaat cggccatggg tgtcgtctcg gccatcaagc cgcccaaccc ggaagcaacg
     3481 agtccaggaa caagagtgtc tgctgccttg taattttcag cattcctttg gatcaaccag
     3541 accggcgctg cgggccacaa tttcgtaatt tccgcccttc gcgacggcgg tatacatctt
     3601 catcttgcag tgacgctgcc ccggcaccat ttccgccggc ccgcccggac cttcagcgat
     3661 ttttgcatgg tcgaag
//
LOCUS       DASWGM010000048         3967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_695525,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000048 DASWGM010000000
VERSION     DASWGM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695525"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VGA15_01640"
     CDS             complement(<1..1045)
                     /locus_tag="VGA15_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9586435.1"
                     /translation="MTRELQRDPQSVISRRTLVHGLAIAAGATVVGTSGALAQQGGEL
                     GPPSTITSPPRDFGPGGAPTTYFWDPDIIAVDPSFNDLAQPNTAIKRLYTGLLWAEGP
                     AWSAQGRYLLWSDIPNNRQMRWSEDDGRVSVFRSPSNNSNGNSFDFQGRQLSCEHLTR
                     RVVRYEHDGTVSVLADLFTGKKLNSPNDVVAHPDGSYWFTDPPYGGQLYEGEPDAPGG
                     ASNPAGKLNPRIGQPAGFVPGKRELPTNCYRVDPSGRIDLVVSEDQVPDPNGLAFSPD
                     YKKLYVVSTGKGPGDTGPGGKGDMFVFDVGADNKLTNQKRFSDFMIDGVKCGPDGVRC
                     DVNGNVWASSNAGR"
     gene            1495..1623
                     /locus_tag="VGA15_01645"
     CDS             1495..1623
                     /locus_tag="VGA15_01645"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HEX9586436.1"
                     /translation="MCYFTSYRRCQASASGTVSFCGTNRRFAHGSRPRGRRPYQVR"
     gene            1869..1973
                     /locus_tag="VGA15_01650"
     CDS             1869..1973
                     /locus_tag="VGA15_01650"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586437.1"
                     /translation="MSASGRGAECLINPYFASAQVPREPHYRRHRRVY"
     gene            complement(2473..2754)
                     /locus_tag="VGA15_01655"
     CDS             complement(2473..2754)
                     /locus_tag="VGA15_01655"
                     /inference="COORDINATES: protein motif:HMM:NF019501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEX9586438.1"
                     /translation="MTGLNRLVAAALISTISATSAFAQAAISEPAAFQAMYPNRDVLN
                     GGALIGSAANNAYAGMASASPSSCAQRHHSYDPATATFLGHDGRRHACR"
     gene            complement(3143..3586)
                     /locus_tag="VGA15_01660"
                     /pseudo
     CDS             complement(3143..3586)
                     /locus_tag="VGA15_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(3854..>3967)
                     /locus_tag="VGA15_01665"
     CDS             complement(3854..>3967)
                     /locus_tag="VGA15_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HEX9586439.1"
                     /translation="GLSGTGAVYVSGGAEHQGPPGGGPVAAIARLSTKTSE"
BASE COUNT          765 a         1165 c         1219 g          818 t
ORIGIN      
        1 ggcggccggc attgctcgag gcccagacat tgccgttgac atcgcagcgc acgccgtccg
       61 gtccgcattt cacgccgtca atcatgaaat cgctgaaccg cttctgattg gtgagcttgt
      121 tgtcggcgcc gacatcgaac acgaacatgt cacccttgcc gcccggacca gtatcgcccg
      181 gccctttgcc ggtactgacg acgtacagct tcttgtaatc gggcgagaac gccagcccgt
      241 tgggatccgg cacctggtcc tcgctcacca cgagatcaat gcggccgctg ggatcgacgc
      301 gatagcagtt ggtcggcagc tcgcgtttgc ccggcacgaa gccggccggc tggccgatcc
      361 gtggattgag cttgccggca ggattgctgg cgccgcctgg agcgtcgggc tcgccctcgt
      421 agagctgacc gccatagggc gggtcggtga accagtagct gccatccggg tgggcgacga
      481 cgtcgttggg cgagttgagc tttttgcctg taaagaggtc ggcgagcacg gacacggtgc
      541 cgtcgtgctc gtagcgcacc acccggcgtg tgagatgttc gcaggagagc tggcggccct
      601 gaaagtcgaa cgagttgccg ttagagttgt tggacggcga gcggaacacg ctgacgcgac
      661 cgtcgtcctc cgaccatctc atctgccgat tgttgggaat gtcgctccac agcagatagc
      721 ggccctgcgc gctccaggcc gggccttcag cccacagcaa tccggtatag agacgcttaa
      781 ttgccgtgtt gggttgggcg aggtcattga acgacggatc gaccgcgatg atgtcggggt
      841 cccagaaata ggtggtcggg gcgccgccag ggccgaaatc gcgcggcggg ctggtgattg
      901 tcgacggcgg ccccagctcg ccgccctgct gggcgagcgc accgctggtc ccgaccacgg
      961 tggcaccggc tgcgatcgcc aggccgtgga cgagcgttcg tcgtgaaatc actgattgcg
     1021 gatcgcgctg cagttcgcgt gtcatcacat cctcccaatg tatgcgccgg ccggttgatc
     1081 cggccgagcg agtttaagac taatccggtc cgggtccggt tgcgagagct gctttcgatt
     1141 cagttcgcag ccgaagcgtg caatgcaaga tggcattgcc tcgtccgccg tcgcgccggg
     1201 tcgagccgct cgctgcgctt tgtcgcaacg tcagaatttc tctcctcgtc gaaattgagt
     1261 atgctgaact gagcatgacc aaacgtttcg acactcccgg caggagtgct gatagaagct
     1321 ggcggcacaa ggtggtactc gcaaaaggct agtggctgag ccagacggat ttggcatcat
     1381 gaggagaaaa ttgaatgcac aagatcattg gagctgaagc gtttgcggcc atggtgttca
     1441 cagcattacc ggcatccgca aagtgatcca aggccgagcc tacggctatt cggcttgtgt
     1501 tatttcacaa gctaccggcg gtgccaggct tcggcctccg gaacagtttc gttttgtggc
     1561 acgaaccgtc gatttgcgca cggatcgcgg ccgcgcggcc gacgacccta tcaggtacgg
     1621 tgatcgttct ggcacttatc gggcttgacg ctcgcgctcg cgacgctttc gatgcgctgt
     1681 gacgtcaatc ccgagggagg ttctgatgcg cattccggct ttggcgattt tgacgacggt
     1741 cacggttttg acggcagcgc cgactctggc ccagacttat agcccggatt atccggtctg
     1801 cctgcaggcc taccgttggg ggggaagcga catcgaatgc ggctataccc tcgctggcgc
     1861 agtgcgccat gtcagcatcg ggccgcggcg ccgaatgcct catcaatcca tattttgcga
     1921 gcgcgcaagt gccccgggaa ccacattatc ggcggcatcg tcgcgtctac taaatatcat
     1981 cttgcgtcgg cttgtccggt gtcatctatc aggtatggat cagtttcatc gcacaatccc
     2041 gtaagccttg gcccgcttcc ggcgcggtgc ccgatgccgc cttcggctca tgcctttgca
     2101 tgcgcccgca tcacgccgat ccgggcgaac atcgttggcc tgtcgtgctc tgcgaccaag
     2161 agaagcgcct gcatcggagg cttgccatac gtccgcatcg tgttctgcct tgtgttctgc
     2221 cttgggcagt ttggtgatga agagcgcggc gtcgcgacca gcggttttcg gcccggtagc
     2281 atgatcggct cgaaagaccg ctgatctcat gacaagagtt taccccacta cgcgaaccgg
     2341 tcccgatgcg acgcgaccat gtccgagact acacgcaaaa cgggcatgtg gcgaacaaac
     2401 caaaatcgac gctaatgatc cagagcggac aggggtgcgt cctgtcgttc aggttcacct
     2461 tgtgactcgc attcaccggc atgcatggcg gcgaccgtca tggccgagga aggtggccgt
     2521 tgcggggtcg taggagtgat ggcgctgggc gcaggatgag ggactagcgc tggccattcc
     2581 cgcataagca ttgtttgccg cagaaccaat tagcgcacct ccattcagca catcccgatt
     2641 tggatacatc gcctgaaaag ctgccggttc tgaaattgca gcctgcgcaa atgcagacgt
     2701 tgccgagatt gtcgatatca gagctgccgc gacgagcctg ttgagtccag tcatagtgat
     2761 tctccatttt ccctttttgc gcgagaattc gcgctggcga ctgctatatg aaaagccaat
     2821 accacttggc tccttaggtt tgcggattta gcttccggcg attgccgagc cacagagtcg
     2881 atgagatggc ttgctattcc gcatcaatca agaaactgga cgggcgatcg atttattccg
     2941 gcgaacgtcg cttgctggga taagcatcgg tctcgacgac ctaacattcg cgtgaatgca
     3001 attcatcgat cacaaaggtg tgtcgctaca tgaggcacgt gcatatgcgg cgacgtatct
     3061 ccctccctat tctcggccgg gcggtcgcgt tgcctcttac agcacgcgga cagcagccac
     3121 agatctgcat gcaaattccc cagaacgccg cctgattccg catcgcgaac gcgtcgaact
     3181 tcgaaccggc attgccggcg ttagtgccgc tgatctgctt ggcgacgtcg ccgtgcagat
     3241 agtcgaacat caagcggacg ttgcgattga cataccagtt cagcgccgcc gtataattgg
     3301 cctgtcgtcc gcggacgaag ccgttggcgg tggcgagctg atcattgcga tcgatagtgc
     3361 tgacacgacc ggcaatttcc cacgcgcccc agccgccacc ggtcaatgac aacggacttg
     3421 ccggcacaat gccgccgaaa gcggcgctgc ccgggttata ctggcgggtt tcgccggtca
     3481 ggacgtaggc tgtctccgca tagccgccct ggaatttcgg gccgggcagc ggggtgaagg
     3541 cgttgcggtc gacgttgaac cagaaatatt cgccttggag gaacagcgta cgtccccgcc
     3601 gcttcggtac gatagcgcgg gagatgatcc tcgagccagt agaaaacgca cagagcgagg
     3661 ctggcccgga cggccgcact gaaataaggg gacgtcgatg gtcatgagaa acgccagata
     3721 gcctacggtc cggatggtcg cgatggcaga gagatataac gcatgtgttg cgtcgaccgg
     3781 ttgagctggc agcgcaaagc ggccattctt cgaacaccaa tatcggcaaa gtgccacaag
     3841 atacgttcag tgctcattca gaagtctttg ttgatagccg agcgattgca gctacgggtc
     3901 cgccgcccgg cggaccctga tgctcggcgc cgccggaaac atagacggcg ccagttcccg
     3961 acaagcc
//
LOCUS       DASWGM010000049        10700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1942555,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000049 DASWGM010000000
VERSION     DASWGM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1942555"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VGA15_01670"
     CDS             complement(<1..950)
                     /locus_tag="VGA15_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9586440.1"
                     /translation="MRYLAMALMTGSMALSAGQAKAADEITIAVFTKNLTNPSYQAFR
                     IAADQIAQAAGVRVIHFVPKQPDNVGEQKAMVEQVLKDKPDAVIFIPVDDVAMIPSVK
                     ELNEAKIPVILVTNPLPGSFVTYVGADDYEIGYREAHYLFDTLGGRGKIVVIEGIPAA
                     ATNRERVRGYKRAVAEYPGIEVLGSGNGNYQQPDAKRVMEKFLAAYPQIDAVLSANDG
                     MALGALEALKEAGRTSIVIGVNGILPAVKQIETGAILASVDFNMFKIGCTATRAAVRY
                     LKREPLPEKVILPAEIIDKTNYKAWLVPVEQRTCPEWAEVV"
     gene            complement(967..3507)
                     /locus_tag="VGA15_01675"
     CDS             complement(967..3507)
                     /locus_tag="VGA15_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9586441.1"
                     /translation="MKTASFSEKYRPLLFASLALIALPFVLRLLGLSLNTGTMVLTFA
                     IATMGLNLCVGYTGLVSFGHGTWFGIGAYAAGLIQLHWFGGEIWLPLLLSMAVVAVAS
                     AIVGIIILRRRGVYFSLLTLAFAALTYTVAFRWSEVTGGEDGLGGLKRGSIGSFSLDN
                     ALAYYITVALIALGVLYVLLRLVRSPFGRVLMAIRENQLRATFQGYPVERYKLVIFVI
                     SAVVTGLAGALLAFQTYLVSAEAVSVPFSGELLAMVVIGGMRNMLGPALGALFFILFR
                     ELFSIWTPNWLLWFGLIFVAFVMYSPGGLVGIWGTLSRRWWPAPEEGAAMSKRKIYEG
                     LPLPGFLMPEGLKGTVLEVNGISKHFGGIRAVTKASLAVGAGEIHALIGPNGAGKTTL
                     FNLISGLFTADGGTIRLNGREIQGVPSNLICRRGLARSFQITNLFRELSIYENLRLSL
                     QAQHGIGFNFWLDIDSYPQVHAETAELIKFLGLEGIEQIEGGELSYGGQRLVDLGIAL
                     GSKPQVLLLDEPLAGLAAAERERVSSLIKNIAANIPVLIVEHDIDRVLGFSQAVTVMN
                     QGEVLMTGSPEAVRSDRRVQEIYTGTGIPEVEHSRTDQAGGEAAQILRFEGVNTFYGK
                     SHILNDATLDVREGEIVALLGRNGAGKSTLLKTLAGLVPLASGTIEYDGNNITGLPAP
                     DISRLGIGYVPQGRGLFAGMTVRENLSLGRLARKTDGSSGVVWSEDQILDYFPRLKER
                     LDVAADYLSGGEQQMLAVARAMSGNVKLLLLDEPFEGLAPTVILELFKVFDLLRRHMS
                     IVIVEHNLDLVLALADRVFALERGAVFHQGPAAPLLTDLEYRKKILWL"
     gene            complement(3504..4379)
                     /locus_tag="VGA15_01680"
     CDS             complement(3504..4379)
                     /locus_tag="VGA15_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9586442.1"
                     /translation="MQFGFLLEQVVNGLVLGGYYLLIALGLSLIFSVGGVVNLAHGAF
                     YALGAYASVEITKYLGFGPAVVLSPIAVAMLGILFERYILRRFYTADPILSLLVTFGL
                     AMIAEQAIRIVWGAAPLSASIPPVFRGSVIVGDFLFSRYRVMLLAVVAVVLIGVWLLL
                     HKTSFGRVVRAGIQRPDMVAALGIRLQPYMTAVVMLGVGMAAMGGAFFAPITIVHPAM
                     GNEIITVAFVVVVIGGLGSFWGVVIAALLVGVVRGITIHFAPAAGEASIYVLMFLVLL
                     VRPRGLLGERIEKFE"
     gene            complement(4474..5712)
                     /locus_tag="VGA15_01685"
     CDS             complement(4474..5712)
                     /locus_tag="VGA15_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586443.1"
                     /translation="MTRYNRRTLLKAGAAFAGASAIGFPAISYGQAEKIKIGHLTPLT
                     GFLGALGTYATLGMKLAVEEINQGGGIMGRQLDVMSEDSVNPATAATKAQRMFEQDGA
                     TVLIGEINSAAALTIMQVADRNKRLFLQTGARSDALRGKNCNKFTFHMDIPNTVMVNT
                     VGKALMRDNMVKGKKFFTLTADYIFGHDLLAAAKRFFGANQGNLIGDELIATDVTDFS
                     PYLLKIRQAKPDVVCSNLAGNQVTTLIKQYAEFGLPYPIVGFNLNTADAWAAGDGNLS
                     GTWPTVWYHTLDVPASKRFVANFIKKNGKPPENHAWIDYVSFKMMAQAMNETKSTDTD
                     KLIAYFEKETEFDILKARKAHFRAWDHQLMQEAYPFTVRPRGQAKDQWDFIALGTALP
                     GAGEPLESLAPTREQNPCTL"
     gene            complement(5858..6751)
                     /locus_tag="VGA15_01690"
     CDS             complement(5858..6751)
                     /locus_tag="VGA15_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9586444.1"
                     /translation="MGPHTKQKTSEKPASPGKAAVAKLVQPPRGDIDDDADDRQRVGV
                     QSLGRAFAILEEVARHREGIGLADLSKLVDLHNSTTFHLAKTMVSLGYLRQEKDSKRY
                     RVGRPLFALAASALDEIEMVNVATPVLEDLSRETGESGHFAVRMGDAVVVIARTSGPG
                     AFQLTDRVGVVRPAHCTALGKIILASLRPDQLKRFLERVDMKPSTAKSITEIPVLLRE
                     IAEIKRSGVAFDDGEFNPEVRCVAVPVTDFTGQVIGALGISGPIWRLSNQALHSSAQI
                     VQAAANRLSAEFGAKTAAKSS"
     gene            complement(6856..8313)
                     /locus_tag="VGA15_01695"
     CDS             complement(6856..8313)
                     /locus_tag="VGA15_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9586445.1"
                     /translation="MSPASAAKFTAEAGVAEIPMLIGGEWRTAAESYEVRDPYRNTVV
                     AKAPRSALTDLDDALDAAVKAKAKAAATPAFERAALLRRAAKLLVERADMIAEIMSRE
                     TGKAIKDAKAEIIRSQDTLNLSAEEAVRIEGEHIPLDASAMGAGKICFMLRFPVGVVA
                     GITPFNAPVNLACHKIAPSIAAGNTLVLKAPPQSPGVIHALAQIFVDAGTPAGVLNVL
                     YGDIVGPALVSDPRVDFITFTGSPRAGAQIKAASGLRRVALELGGNGVTIVHQDADIA
                     EAAPVCARNSMRLAGQSCISVQTVYVHRSVYEKFLDIVVTEVRKLKLGDPLDATTDVG
                     TLIDEGAAQRVESWIKEAADGGAKVLTGGRRHGAQIEPTVIAGVKPVMKVVCDEVFGP
                     VISLLPYDDIDEVFRTVSESRFGLQTGIFTKSMELAIRAVRNLRTGGVIINGSSTWRT
                     DQLAYGGVKDSGIGREGPRYSIRDMTEERMVLFNY"
     gene            8558..10360
                     /locus_tag="VGA15_01700"
     CDS             8558..10360
                     /locus_tag="VGA15_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9586446.1"
                     /translation="MSPRNALNGAQVIVDYLIQEKVPQVFGLCGHGNIQFIDALYERA
                     DELKTISVHHESVAGFMADVYYRVSGRPTATFASCGPGSANLPISLGNAFLDSVPFLA
                     VTGNVPTSQFNRGAFQELYRHYQADFPSTVRSYCKKVFQPTRGEMVPLAIRQAWKTMT
                     TGRPGPVVLDVPFDVFLESAAEEAPNAQAWNCNISSRCGADPEGVVKAVDMLLGAERP
                     VMIVGQGVRYGGAADELLKLAERLQIPVAASASGLGAIDCNHPLALGLVARAGHYQAN
                     HATRQADVLLAMGMRFDDRTSSSWISGYSFTIPPTRLIHVDIDPEEIGRNYPVALGLM
                     ADVRTFLRQIHAELDRRADLTRQADARRKWLAQIDTYRKEWDKFVAPGFTDDTIPINP
                     QRAALEIDKALPENAILVSDIGVHHNWLLSFCKPKRPDSLIGSMGFGPMGFGVAGVMG
                     AKFAAPDRPCVSVCGDGAFFMHANVLGTAVEYNLPVVWVVWNNYAYASIRGLQRGYLG
                     GRELATDFHDPITGARYNPDFAAMARSCGVEGVRVDRAGDLGEAIRKGIAANKPYLID
                     VDIAADINPSGAGIWELPGLGRSKPGIGTQFEPT"
     gene            10424..>10700
                     /locus_tag="VGA15_01705"
     CDS             10424..>10700
                     /locus_tag="VGA15_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9586447.1"
                     /translation="MLAGKKVVVVGGSSGIGLATAELAKREGADVILASRNVDRLDAV
                     AAKLNAIAIPADVTSDESIVNLFRRCGPVDHVVVTAAQLRSGPFKTVS"
BASE COUNT         2009 a         3373 c         3329 g         1989 t
ORIGIN      
        1 accacttcgg cccattccgg gcaggtacgc tgctccaccg ggacaagcca ggccttgtag
       61 ttggtcttgt cgatgatctc ggccggcagg atgacctttt ccggcagcgg ctcgcgcttg
      121 aggtagcgca ccgcggcgcg ggtcgccgtg cagccgatct tgaacatgtt gaaatcgacg
      181 gacgcgagga tcgcgccggt ttcgatctgc ttcaccgccg gcagaatacc gttgacgccg
      241 atcacgatgg atgtccggcc cgcctccttc agcgcctcca aggcgccaag cgccatgccg
      301 tcattggccg agagcacggc gtcgatttgc ggataggcag ccaagaattt ctccatcacg
      361 cgtttggcgt cgggttgctg atagttgccg ttgccggagc caagaacctc gataccggga
      421 tattcggcga cggcgcgctt gtagccgcgc acgcgttcgc gattggttgc cgccgccggg
      481 atgccttcga tgaccacgat ctttcctctg ccgccgagtg tgtcgaacag atagtgcgct
      541 tcccggtatc cgatctcgta atcgtcggcg ccgacatagg tgacaaaact gccaggcagc
      601 ggattggtaa ccagtatgac cgggattttc gcctcgttga gttccttcac cgacgggatc
      661 atggcgacgt catcgacggg gatgaagatg accgcgtcgg gtttgtcttt gaggacctgc
      721 tccaccatcg ccttctgttc cccgacattg tccggctgct ttggaacgaa gtggatcacc
      781 cgcacgcccg ccgcttgcgc aatctggtcg gcggcgatgc ggaacgcttg ataggacgga
      841 ttggtcaggt tcttggtgaa caccgcgatg gtgatctcat cagccgcttt ggcctgaccg
      901 gcggacaacg ccattgaacc ggtcatcagt gccattgcca gatatcgcat ggtcgcctcc
      961 ttgaggtcac agccacagga tcttcttgcg gtattcgagg tcggtcagca gcggcgccgc
     1021 cggcccctgg tgaaacacgg cgccgcgctc cagcgcgaac acgcggtcgg ccagcgccag
     1081 caccagatcg agattatgct ccacaatcac gatagacata tgtcggcgca gcaggtcgaa
     1141 cactttgaat agctctaaga tcacggtcgg ggcgaggccc tcgaacggct catccagcag
     1201 caacagtttg acgttgcccg acatcgcacg cgccaccgcc agcatctgct gctcgccgcc
     1261 ggacaggtaa tccgccgcga catccagtcg ctctttcagg cgcgggaaat agtcgaggat
     1321 ttggtcctcg ctccacacca cgccgctact gccgtcggtc ttgcgtgcca gacggccgag
     1381 cgagagattt tcccgcaccg tcatgcccgc gaacaggccg cgtccctgcg gcacgtagcc
     1441 tatgcctagg cgcgagatat ccggggccgg cagcccagtg atattgttgc cgtcatattc
     1501 gatggtgccg gaggccagcg gcaccagccc ggccagcgtt ttcaaaagcg tggacttgcc
     1561 ggcgccgttg cgccccagca gcgcgacgat ctcgccttcg cgcacatcca gggtcgcgtc
     1621 gttcaggata tggctcttgc cgtagaaggt gttgacgcct tcgaaacgca ggatttgcgc
     1681 cgcttcaccg ccggcctgat cggtgcggct gtgctcgacc tcggggatgc cggtaccggt
     1741 atagatttcc tgcacgcgcc ggtcggaacg caccgcctcc ggcgaacccg tcatcagcac
     1801 ttcaccctgg ttcatcacgg tgaccgcttg cgagaaacca agcacgcgat cgatgtcgtg
     1861 ttcgacgatc aggaccggaa tattcgctgc tatgttcttg atcaggctcg acacccgttc
     1921 acgctcggcg gcggcgaggc ccgccagcgg ctcatcgagc aacaggacct gcggtttgga
     1981 gcctagagcg atgcccagat ccaccagccg ctgcccgcca taggacagtt cgccgccctc
     2041 gatctgctcg atgccttcga ggccgaggaa ctttatcagt tccgcggtct cggcgtgcac
     2101 ttgcggatag ctgtcgatgt cgagccagaa gttgaagccg atcccgtgct gggcctgcag
     2161 cgaaagccgc aaattttcgt agatcgacag ctcccggaac aggttggtga tttgaaacga
     2221 acgcgccagt ccgcggcggc agatcagatt ggatggcacg ccctggatct cgcggccatt
     2281 gagcctgatc gttccgccgt cagccgtgaa caggccagag atcaggttga acagcgtggt
     2341 cttgccggcg ccgttcggac cgatcagggc atggatttca ccggcgccga ctgcaagact
     2401 ggccttggtg acagcgcgga tgccaccgaa atgttttgag atgccattga cctccagcac
     2461 ggtgcctttg aggccctcgg gcatcagaaa acccggcagc ggcaggcctt cgtagatctt
     2521 gcgcttgctc atggcggcgc cttcttccgg cgcgggccac cagcgccggc ttagcgtgcc
     2581 ccagatgccg accaggccgc cgggcgaata catcacgaag gcgacgaaga tcagcccgaa
     2641 ccaaagcagc cagttcggcg tccagatcga aaacaattcg cggaacagga taaagaacaa
     2701 cgcgccgagc gcgggcccaa gcatgttgcg catgccgccg atcacgacca tcgccaacag
     2761 ctcgccggaa aacggtaccg aaaccgcttc ggcggaaacc aggtaggtct gaaacgcgag
     2821 cagcgcgccg gcgagcccgg tcaccacggc cgagatcacg aagatcacga gcttgtaacg
     2881 ttcgaccgga tagccctgga aggtggcgcg cagctggttt tcgcggatcg ccatcagcac
     2941 acggccgaac ggcgatcgca ccaggcgaag cagcacataa agcacgccca acgcgatcag
     3001 cgcgacggtg atgtaatagg cgagcgcatt gtcgaggctg aacgacccga tgctgccgcg
     3061 cttcaagccg cccagaccgt cttcgccgcc ggtcacctcg ctccagcgga acgcgacggt
     3121 gtaggtgagc gcggcaaacg ccagcgtcag cagcgagaag tagacgccgc ggcgccgcaa
     3181 gatgatgatg cccacgatcg ccgacgcgac cgcaaccacg gccatcgata gcagcagcgg
     3241 caaccatatc tcgccgccga accagtgcag ctggatcaat cccgcggcat aggcgccgat
     3301 gccaaaccag gtgccatggc cgaacgatac cagtccggtg tagccgacac acagattgag
     3361 ccccatggtc gcgatcgcaa acgtcaacac catggtcccg gtgttcagcg agagaccgag
     3421 cagcctcagg acgaacggca gagcgatcaa cgcgaggctt gcgaacagca atggccggta
     3481 tttttcgcta aatgaggcgg tcttcattcg aatttctcga tacgctcgcc gagcagcccg
     3541 cgcggacgca ccagcaacac cagaaacatc aagacataga tcgatgcttc gccggcggcg
     3601 ggggcgaaat gaatggtgat gccgcgcacg acgccgacca gcagtgccgc gatcacgacg
     3661 ccccagaaac tccccaggcc cccgatcacc acaacgacaa aggcgacggt gatgatctca
     3721 tttcccatcg ccggatgaac gatggtgatc ggcgcaaaga aggcgccacc catcgcggcc
     3781 atgccgacgc cgagcatgac gacggcggtc atgtagggct gcagcctgat gcccagcgcc
     3841 gcgaccatgt ccggccgctg aatgccggcg cgcaccacgc ggccgaacga ggtcttgtgc
     3901 agcaacagcc agacgccgat cagcaccacc gccaccaccg ccagcagcat cacgcggtag
     3961 cgcgaaaaca ggaagtcgcc gacgatgacc gagccgcgaa acaccggcgg tatcgatgcc
     4021 gacaagggtg ccgcgcccca cacgatacgg atcgcctgtt cggcgatcat ggcgagaccg
     4081 aaggtgacca gcaggctcag gatcggatcg gcggtgtaga aacggcgcag gatgtaccgt
     4141 tcaaacagga tgcccagcat cgcgaccgcg atcggcgaca acaccacggc cgggccgaag
     4201 ccgaggtatt tggtgatttc gaccgatgca taagcgccga gcgcgtaaaa cgcgccgtgc
     4261 gccaggttga ccacaccgcc gacgctgaag atcagcgaca ggccgagcgc gatcaagaga
     4321 taatagcctc cgagcacgag gccatttacc acctgttcca gcaagaaccc gaactgcatt
     4381 ctcgctccag ggagactctg atgtcagcca atgcgcccaa tacgcgggca ccccgcgcgc
     4441 cctgaacagg gcgcgccggg tgccgacgcg ccgttacaac gtgcaggggt tctgttcgcg
     4501 tgtcggcgcc agcgactcca gcggttcgcc cgctccggga agcgcggtcc ccagcgcaat
     4561 gaagtcccac tgatccttgg cctggcccct gggcctgacc gtgaacggat aggcttcctg
     4621 catcagctga tggtcccacg cccggaaatg cgccttgcgc gccttcagga tgtcgaactc
     4681 cgtctctttc tcgaaatagg cgatcagctt gtcggtgtcg gtcgacttgg tctcgttcat
     4741 ggcctgcgcc atcatcttga acgaaacata gtcgatccag gcatggttct cgggcggctt
     4801 gccgttcttc ttgatgaagt tggccacgaa acgcttggag gccggaacgt cgagggtgtg
     4861 ataccagacc gtcggccagg tgccgctgag attgccgtcg ccggccgccc aggcatccgc
     4921 cgtattgaga ttgaagccga cgatcggata gggcaggccg aattcggcat attgcttgat
     4981 cagggtcgtc acctgattgc ctgccaggtt tgaacagacc acgtcgggct tggcctggcg
     5041 gatcttgagc agataggggc tgaagtcggt cacgtcggtc gcgatcaact cgtcgccgat
     5101 caaattgcct tgattggcgc cgaagaaacg cttggccgcg gccaacaggt catggccgaa
     5161 aatatagtcg gcggtcagcg tgaagaactt cttgcccttc accatgttgt cgcgcatcag
     5221 cgccttgccg acggtattca ccatcaccgt gttcgggata tccatgtgga aggtgaattt
     5281 gttgcaattc ttgccgcgca gcgcgtcgga tcgcgcgccg gtttgcagga acagccgctt
     5341 gttgcggtca gccacctgca tgatggtcag cgcggcggcc gagttgatct cgcctatcag
     5401 cacggtagcg ccatcttgct cgaacatgcg ctgggccttg gtcgcggcgg tagcgggatt
     5461 gaccgaatct tccgacatga cgtccagctg gcgccccatg atgccgccgc cctggttgat
     5521 ttcttccacg gccagtttca taccgagggt ggcgtaagtg ccgagcgcgc cgagaaagcc
     5581 ggtcagcggc gtgagatggc cgattttgat cttttcggcc tggccgtagc tgatggccgg
     5641 aaatccgatt gcggaggcgc cggcaaaggc ggcgcccgct ttcaataagg ttcgtcggtt
     5701 gtaacgggtc atcgttggtc tcctcccaag agcgcatccg cgatgtcctc ctccgattcc
     5761 ggcccatgcg agaatgtgag aggcggtttt ttattgttga atgatatttt cggaggcagg
     5821 gcccgctgtc aacgccaaaa gtaccgcccg gctccgttta tgaagatttc gccgcggtct
     5881 tggcgccgaa ttcagccgac aggcgattgg ccgccgcctg aacgatttgg gcgctgctgt
     5941 gcaaggcctg gttggacagg cgccagatcg gaccggaaat accaagcgcc ccaatcacct
     6001 ggccggtaaa atccgtgacc ggcaccgcga cgcaccgcac ctccggattg aattcgccgt
     6061 cgtcaaaggc gacgccgctg cgcttgatct cggcaatttc acgcagcagc accggaatct
     6121 cggtgatcga cttcgcggtc gagggtttca tgtcgacgcg ctcgagaaaa cgcttcagtt
     6181 ggtcggggcg cagcgaggcg aggataatct tgccgagcgc ggtgcaatgg gccggccgca
     6241 ccacgccgac gcggtcggtc agttgaaatg cgccggggcc gctggtgcgg gcgatcacga
     6301 cgacggcgtc gcccatgcgt accgcgaaat ggccgctttc gccggtttcc cgcgacaggt
     6361 cctccagcac cggcgttgcg acattgacca tctcgatttc gtcgagcgcg ctggccgcca
     6421 gcgcaaacag cggccggccg acgcggtagc gctttgaatc cttttcctgg cgcagatagc
     6481 cgagcgacac catcgttttg gcgagatgga aggtagtgga gttgtgcaga tcgaccagct
     6541 tgctcaggtc tgccagcccg ataccttcgc ggtgacgcgc gacctcttcg aggatggcga
     6601 aggcgcggcc gagcgactga acgccgaccc gctggcgatc gtcggcgtcg tcgtcaatat
     6661 cgccccgcgg cggctgcacc agcttggcga cggcggcctt gcccgggctg gccggcttct
     6721 cactggtctt ttgcttggta tgcggtccca caggatttag cctccacgcg acggtgccga
     6781 attgtgctgt cgccctatca ttggccgcgg cggccgttca tacaaccccc gtgcaggggg
     6841 cggcccaata cagtcctaat aattgaacag caccatgcgt tcctcggtca tgtcgcggat
     6901 cgaatagcgc gggccctcgc ggccgatgcc tgaatccttc acgccgccat aggcgagctg
     6961 gtcggtgcgc caggtcgagc tgccgttgat gatgacgccg ccggtgcgca ggttgcgaac
     7021 ggcacgaatg gcgagctcca tcgatttggt gaaaatgccg gtctgcaggc cgaaccggct
     7081 ttcgctgacg gttcgaaaaa cctcgtcgat gtcgtcatag ggcaacaggc tgatgaccgg
     7141 cccgaacacc tcgtcgcaca ccaccttcat gacaggtttg acgccggcaa tcacggtcgg
     7201 ctcgatttgg gcgccatggc gcctgccgcc ggtgaggacc tttgcgccgc catcggcggc
     7261 ctccttgatc caggattcca cccgctgggc tgcgccttcg tcgatcagcg tgccgacgtc
     7321 ggtggtggca tccaagggat cgccgagctt cagtttcctc acctcggtga cgacgatatc
     7381 gaggaatttc tcatagacgc tgcggtgcac atagacagtc tgcaccgaaa tgcagctttg
     7441 cccggcgagc cgcatcgagt tgcgggcgca gaccggcgcc gcttcggcga tatcggcatc
     7501 ctggtggacg atggtgacgc cgttgccgcc taattcgagc gcgacccggc gcaagcccga
     7561 cgccgccttg atttgcgccc cggcccgcgg agaaccggta aaggtgatga aatcgacgcg
     7621 cgggtcgctc accagcgccg gaccgacgat gtcgccgtac aggacgttga gcacaccggc
     7681 cggagtgccg gcgtcgacaa agatctgcgc cagcgcgtga ataacgcccg gtgactgcgg
     7741 cggcgccttc agcaccagcg tatttccggc cgcgatcgaa ggcgcgatct tgtggcaggc
     7801 gaggttcacc ggcgcattga acggcgtgat ccccgccaca acgccgaccg gaaagcgcag
     7861 catgaaacag atcttgccgg cgcccatggc gctggcgtcg agcgggatat gttcgccctc
     7921 gatgcgcacg gcctcttcgg ccgacaaatt gagcgtgtcc tgggagcgga tgatttcggc
     7981 cttggcgtcc ttgatcgcct tgccggtttc ccgggacatg atttcggcaa tcatatccgc
     8041 gcgttcgacc aataacttcg cggcgcggcg caacagcgcg gcgcgctcaa aggcgggtgt
     8101 cgcggcggct ttggccttgg ctttgaccgc cgcatcgagc gcatcgtcga ggtcggtcaa
     8161 ggccgagcgc ggggcctttg cgacgaccgt attgcgatag gggtcgcgca cctcgtagct
     8221 ctccgcagcg gtgcgccact cgccgccgat cagcatcgga atttcggcta cgccggcttc
     8281 ggcggtaaat tttgcggcgg aggctgggct catcttggac ctcccgggtc ggcaaaccag
     8341 gtctggtatt caggtctggt attcctggaa gcggcgctgt tcggcgtctg gcgccgccgg
     8401 tcatcaggct tagccgtgtt cacgggggga agtctaccac aatataaatt gacgtacagt
     8461 attatgggaa atagggtgcc gcgcgcctgg gcagtttcgg cagcgaagat ggctgcgccg
     8521 atctcctcaa atcgtcaaac tggtagggag agatccgatg tcgccgcgca acgcgctcaa
     8581 tggggctcag gttattgtcg actatctgat tcaggagaaa gtgccgcagg tgttcggcct
     8641 gtgcggtcac ggcaatatcc agttcatcga tgcgctgtat gagcgcgccg acgagctcaa
     8701 gaccatctcg gtgcatcacg agagcgtcgc cggcttcatg gcggacgtct attaccgggt
     8761 ttcggggcgg ccgaccgcca ccttcgcatc ctgcgggccg ggttcggcga acctgccgat
     8821 ttcgctcggc aatgcgtttc tcgactcggt gccgttcctg gcggtgaccg gcaacgtgcc
     8881 gaccagtcag ttcaatcgcg gcgcgtttca ggaattgtac cggcattatc aggcggactt
     8941 tccctccacg gtacgctcgt actgcaagaa ggtgttccag ccgacccgcg gtgagatggt
     9001 gccgctcgcg atccggcagg cctggaagac catgaccacg ggccgtccgg gtccggtcgt
     9061 gctcgacgtt cccttcgatg tgtttctgga atcggctgcc gaggaagcac cgaacgcgca
     9121 agcatggaac tgcaatatct cgagccgctg cggcgccgat ccggaaggcg tcgtcaaggc
     9181 ggtcgatatg ctgctcggcg ccgagcggcc ggtgatgatc gtcggccagg gtgtgcgcta
     9241 cggcggcgcc gccgacgagt tgttgaaact cgccgagcgc ctgcaaattc cggtggcggc
     9301 ctcggccagc ggccttggcg cgatcgattg caatcatccg ctggcgcttg ggctggtcgc
     9361 gcgcgccggc cattatcagg ccaatcacgc gacgcgtcag gccgacgtgc tgctggcgat
     9421 gggcatgcgc tttgacgatc gcacttccag ttcgtggata tcaggttatt cgttcacgat
     9481 tccgccgacc cgcctcattc acgtcgacat cgatccggag gaaatcggcc gcaactatcc
     9541 tgtcgcgctc ggcctgatgg ccgatgtgcg caccttcctg cggcagattc acgctgaact
     9601 cgaccgccgc gccgacctca ccaggcaggc cgatgcccgc aggaaatggc tggcgcagat
     9661 cgacacctat cgcaaggaat gggacaagtt cgtcgccccc ggttttaccg acgacaccat
     9721 accgatcaac ccgcagcgcg cagcgctgga gatcgacaag gcgctgcccg agaatgcgat
     9781 tctcgtcagc gacatcggcg tgcaccacaa ttggctgttg agtttctgca agccgaagcg
     9841 gccggactcg ctgatcggct cgatgggttt tgggccgatg ggttttggcg tcgccggcgt
     9901 gatgggcgcg aagttcgccg cccccgaccg gccctgcgtg tcggtatgcg gcgacggcgc
     9961 gttcttcatg catgccaacg tgctgggaac cgcggtcgaa tataatctgc cggtggtctg
    10021 ggtggtctgg aacaattacg cctatgcctc gatccgcggg ctgcagcgcg gttatctcgg
    10081 cggccgcgaa ctcgccaccg acttccacga tccgatcacc ggcgcgcgtt acaatccgga
    10141 ttttgccgcg atggcgcgct cctgcggcgt cgagggcgtg cgggtcgatc gcgccggcga
    10201 tctcggcgaa gccatccgca agggtatcgc cgccaacaaa ccgtatctga tcgacgtcga
    10261 tatcgccgcc gatatcaatc cgagcggcgc cggcatttgg gagcttcccg gcctcgggcg
    10321 aagcaagccg ggcatcggaa cccaattcga gccgacctga tcgtcattca cgtcggcacg
    10381 acatttctct ccgccaaaat cacaacatca gggagacagc atcatgcttg caggtaagaa
    10441 agtcgtcgtc gttggaggct cttccggtat cggacttgcg accgccgaac tcgccaagcg
    10501 ggaaggggcc gatgttattc ttgcatcccg taacgttgac cgcctggatg cggtggcggc
    10561 caagttgaac gccatcgcga tcccggccga tgtcaccagc gatgaaagca tcgtcaatct
    10621 cttccgccgc tgcggcccgg tcgatcatgt cgtggtcacc gccgcgcagc tccgctcggg
    10681 tcctttcaaa accgtttcca
//
LOCUS       DASWGM010000050         3274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1806058,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000050 DASWGM010000000
VERSION     DASWGM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806058"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VGA15_01710"
     CDS             <1..363
                     /locus_tag="VGA15_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX9586448.1"
                     /translation="IQRRISQRRASLRLDPNAAGGSALNLLTKRKQTTPSSVMVLEAL
                     SQALPDSTYVTELRIEGDKVQVVGMTQDAPSLIRLMERSPQFARATFFAPTTRAQNDP
                     GERFHLEAHITPSFGSGT"
     gene            360..917
                     /gene="gspM"
                     /locus_tag="VGA15_01715"
     CDS             360..917
                     /gene="gspM"
                     /locus_tag="VGA15_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX9586449.1"
                     /translation="MNLAAALRKSFAASPILAAAAYAGLVFALLVMVVTSIVDILDQR
                     AAVASSAALLDQLEGRSTAASGGRSADVLMPSGSAFLEGATVTVAGAALLQRVAGAVT
                     KLGGNVLSSQLDLQGTQSKAGFVSMVASCEIDQPALQPLIYHLEAGMPFLFVEQLAVQ
                     APTSSSGEGKLRILLAVSGQWQGMK"
     gene            914..1588
                     /locus_tag="VGA15_01720"
     CDS             914..1588
                     /locus_tag="VGA15_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586450.1"
                     /translation="MMRKLAIAITVVSVYGGASASTSVIFNDALDADRAGRAQAGPQA
                     FSISANPAAPVQVLVSSPAPVAAASERALSANPLWAIPLSQLPITRDRPIFSPSRRPP
                     PPAVAPAAIPKVAAVPKPREPERPQLTLVGTIASDDEGFGIFLDQSTKVVFRLKVGQD
                     HQGWKLRSVQGREAILEKDQQVVPLALPQLSVGQVTIEVPPPPVNAGKLLSVTSQPRP
                     NDRSGR"
     gene            complement(1894..>3274)
                     /locus_tag="VGA15_01725"
     CDS             complement(1894..>3274)
                     /locus_tag="VGA15_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9586451.1"
                     /translation="SNARTPANSDSFPGLLALVTGGSPISHGLFYDVSYDRTLFDPTN
                     VSCSGGPGNMMVFDESVDRYSSGNVSLNIIDPLKLPRGRNEFGHCVPVYPHDAIRTNT
                     IFEVVRAKGGRTAWADKHPAYDLVNGPSGKGVEDLYTPEITNVGGFDATVSVDCTVAN
                     DQLKVTAIINEINGLKHDGSPNSGVPALFGMNFQAVSVGQKLAKDNSDGSCPQSTHTG
                     LPGGYIDGAGTPTAVLAYGLQKTDEALGSMIQALKQRGLYNSTLFIVGAKHGQSPINP
                     AKVNKPGHFADLVAALPDAATNPGALAIASASACAKGPCGFVQDDDIALIWLQDQNQT
                     KAVVDYLNTNAKALFIDEVLGGDELKLRFNDPAHDSRTPDIIVQPIYGTIYTGSKTKN
                     AEHGGFSFGDTNVGLIVSNPRLPGVVLKTPVVTSQVAPTILHALDIEPRGSELRASRA
                     HFNAARFVE"
BASE COUNT          600 a          990 c         1031 g          653 t
ORIGIN      
        1 atacagcgcc ggatttcgca acgccgcgcg tcgctgcgat tagacccgaa tgccgcgggc
       61 gggtcggcct tgaacctgct gacgaaacgc aagcagacga cgccatccag cgtcatggtg
      121 cttgaagccc tctcgcaggc gctgcccgac agcacctacg tgacggagtt gcggatcgaa
      181 ggcgacaagg ttcaggtcgt cggcatgacg caggatgcgc cgtctttgat ccgattgatg
      241 gagaggtcgc cgcaatttgc ccgcgccact ttctttgcgc ccaccacgcg cgcgcagaat
      301 gacccgggag agcgtttcca cctcgaggcc cacatcacgc cgtctttcgg atcgggcaca
      361 tgaacctcgc ggctgctttg agaaaatcgt tcgcggcgtc gccgatcctg gcggcggcag
      421 cctacgccgg actcgtgttc gcgctgctgg tcatggttgt cacctccatt gtcgacattc
      481 tcgaccagcg ggccgcggtg gcatcgtcgg cggcgctgct ggatcaactc gagggccgca
      541 gcaccgctgc atccggcggg cgatccgccg acgtcctgat gccatccggt tcggcgtttc
      601 tggagggggc gacggtgacc gtcgcgggtg cggccctgct gcagcgggtc gccggcgcgg
      661 tgacgaaact cggcggcaat gttctgtcat cccagctcga tttgcagggc acgcaatcca
      721 aggcgggatt tgtcagcatg gtcgcgagct gcgaaatcga tcagccggca ttgcagccgc
      781 tgatctacca tctcgaagcc ggaatgccgt tcttgtttgt cgaacagctc gccgttcaag
      841 ccccgacctc ctcgtccggt gaggggaaat tgcgaattct gctggcggta tcggggcaat
      901 ggcagggcat gaaatgatgc ggaagcttgc gatcgcgatc acggttgtgt cggtgtacgg
      961 cggcgcatcc gcatcgacat cagttatttt caacgatgcg ctggacgccg atcgggccgg
     1021 tcgcgcgcaa gcggggcctc aggcattttc gatcagcgcg aatccggccg cgccggtgca
     1081 agtcctcgtc tcttcacccg caccggtcgc cgcggcatcg gagcgggcgt tgagcgccaa
     1141 tccgttgtgg gcgatcccgc tctcgcaatt acccatcacg cgcgaccgcc cgatcttttc
     1201 accgtcccgg cggccaccgc cgccagcggt cgcgccggcc gcgatcccga aagtggcagc
     1261 cgtgcccaaa ccaagggaac ccgagcgtcc gcagctcact ctggtcggca ccatcgccag
     1321 cgacgatgag gggttcggga tatttctgga tcaatcgacc aaggtggtat tccggctgaa
     1381 ggtcggccag gaccatcagg gctggaagct gcgatcggtt caaggccgtg aagctatcct
     1441 ggaaaaggat caacaggttg ttcccctcgc attgccccag ctcagcgtgg gacaggtgac
     1501 cattgaagta cctccgccac cggtgaacgc cggaaagctg ctgtcggtga cgtcccagcc
     1561 gcgacccaat gataggtccg gtcgctaatg ttctgtgatc ctctggacgc tcagcaccac
     1621 gtccagtgtt gttgaggcgc cttcagcgcg gcaaccatca caagcacgcg aagcccccga
     1681 tgccaagatc cggcggcttc gcgcggaatg cgcgccggaa ccgaagaggg agaggggtcc
     1741 gaaagccgat gctctcaccc gtcccttaaa agacagtcat caagcgggat gtcatgccct
     1801 ctgactggtt ttgcaaagcg aaagggccct cgaagagggc cctttctgaa ccagacgttg
     1861 ccgagttcca ggtctagtcg tcattactag tcgtcattcc acaaaccggg cagcattgaa
     1921 gtgtgctcta ctcgcacgga gttcagagcc ccggggctca atgtcgagcg catgcaggat
     1981 ggtcggcgcg acctgcgaag taaccacagg ggtttttagg acgacgccgg gaagccgcgg
     2041 attggaaacg atcaacccga cattggtgtc accgaagctg aaaccgccgt gctcggcgtt
     2101 cttcgttttc gatcctgtat agatggtgcc atagatgggc tggacgatga tgtcgggtgt
     2161 gcggctgtcg tgcgccgggt cgttgaacct gagcttcagc tcgtcgccac ccagaacttc
     2221 gtcgatgaac agagccttgg cgttggtatt gagataatcg acaaccgcct tggtctggtt
     2281 ttgatcctgg agccagatca acgcgatatc gtcatcctgc acgaaaccgc agggtccctt
     2341 ggcgcaggca ctggcactgg cgatcgccag agcgccggga ttcgtcgcgg catccggcag
     2401 ggctgccacc aggtcggcga agtgccccgg cttgttgacc ttggccggat tgatcggcga
     2461 ctggccgtgc ttggcgccga cgatgaacag tgtcgaatta tagaggccgc gctgcttcag
     2521 cgcctgaatc atgctgccga gcgcctcgtc ggtcttttgc aggccatagg cgaggacggc
     2581 ggtcggtgtt cccgcaccat caatatagcc gcctggcaaa cccgtgtggg tgctctgcgg
     2641 gcagctaccg tcactgttgt ccttggccag tttctggcca acgctcacgg cctgaaagtt
     2701 cattccgaat aaggcgggaa cgccgctgtt gggcgagccg tcatgcttga ggccattgat
     2761 ctcgttgatg atggcggtca ccttcagctg atcgttagcc accgtgcaat ccacgctgac
     2821 ggtggcgtca aacccgccga cgttggtgat ctccggcgtg tagagatctt ctacgccttt
     2881 gcctgacggc ccgttgacga gatcgtaggc cggatgcttg tcggcccacg cggtgcggcc
     2941 gccttttgct ctgaccactt caaagatggt gttggtcctg atggcgtcgt gcggatacac
     3001 gggaacgcag tggccgaatt cgttgcgtcc acgcggcagc ttcaacggat cgataatatt
     3061 cagcgaaacg ttgccgctgg aatatcggtc gacgctctcg tcgaacacca tcatgttgcc
     3121 agggccgccg ctgcacgaaa cattggtcgg atcgaacaga gttcggtcat agctgacgtc
     3181 gtagaacagg ccgtggctga tcggtgagcc cccggttacc agcgcaagca ggccggggaa
     3241 tgaatcagag ttcgcgggcg tgcgtgcgtt gctg
//
LOCUS       DASWGM010000051         8870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1666998,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000051 DASWGM010000000
VERSION     DASWGM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666998"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="VGA15_01730"
     CDS             <1..1893
                     /locus_tag="VGA15_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9586452.1"
                     /translation="DTYCVNLNKKARDGKIDPVIGRNAEINRAIQVLCRRQKNNPLFV
                     GEAGVGKTAIAEGLAKRIVDSEVPEVLAAATVFSLDMGTLLAGTRYRGDFEERLKQVL
                     KELEAHPNAILFIDEIHTVIGAGATSGGAMDASNLLKPALASGTIRCMGSTTYKEYRQ
                     HFEKDRALVRRFQKIDINEPTVEDAIAILKGLKPYFEDYHRLKYTNEAIEAAVQLSSR
                     YIHDRKLPDKAIDVIDESGAAQMLVAENKRKKTIGIKEIETTIATMARIPPKSVSKDD
                     AEVLKHLEQTLKRVVFGQDKAIDALSASIKLARAGLREPEKPIGSYLFSGPTGVGKTE
                     VAKQLAASLGVELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDGVDQHPHCVV
                     LLDEIEKAHPDLYNVLLQIMDHGRLTDHNGKQVNFRNVILIMTTNAGAADLARQAFGF
                     TRNKREGDDHEAINRQFAPEFRNRLDAIVSFAHLNADVIGMVVEKFVLQLEAQLADRD
                     VTIELSEPAKAWLVQHGYDEQMGARPMARVIQEHIKKPLADEVLFGKLKGGGHVRVVL
                     VKDEAAADVAQEKIGFEYVEGPVTPKPEKLPGTRKRTAPRRQKPPGGPSGGSKGPASR
                     GPLVKV"
     gene            2156..2401
                     /locus_tag="VGA15_01735"
     CDS             2156..2401
                     /locus_tag="VGA15_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9586453.1"
                     /translation="MLANRRQSERRHCRRVAKIQFGTGSLPRDCTITDISEGGLKVIA
                     EYPEIPPQFTIILSTGHPRQCRLAWRIGCEFGAEFID"
     gene            2575..3399
                     /locus_tag="VGA15_01740"
     CDS             2575..3399
                     /locus_tag="VGA15_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9586454.1"
                     /translation="MLQQLLQHYRFAKLLASIASSAILVLGLCSCQTAATSDITGSLG
                     EKTEASRGVDPRREVESYRDRFRANPKDAEAALQYAKALRAAGQQAQAVAVLEQATIA
                     HPGDNALLAGYGRALADNGNFQQAFDVLSRAHTPDDPDWRILSAQGTALDQLGRHDEA
                     RQYYATALKIAPEEPSVLSNLGLSYVLSKDLPKAEEALRRAYSRADADSRVRQNLALV
                     IGLQGRLAEAESIVKAGRPAEEAAANVAYLRRLLARKDNAHTDSDKIPVAAIGRPI"
     gene            complement(3492..4760)
                     /locus_tag="VGA15_01745"
     CDS             complement(3492..4760)
                     /locus_tag="VGA15_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9586455.1"
                     /translation="MISIARQNPEEQPDIAPPPADDYIAPAPRVSVQAFCETAETAAA
                     VRSAGEDRRLSKAHLSIQMGGMAAAIEAYHTVPTPNVIILETEGRSDILAGLDQLATV
                     CDAGTRVVVIGSINDAAPYRELVRRGVSDYVVGPVDTLDVVRSICSLFSASEAVAVGR
                     IIAIVGAKGGVGASTVAHNVAWAIARDLALDSVVIDLDLAFGTAGLDYNQDPLQGIAN
                     AVFSPDRLDTAYMDRLLSKCTEHLSLLAAPATLEQVYDFGANAFDSVFDTLRMTTPCI
                     VLDIPHQWSGWTKRALVGADDILIVAEPDLANLRNTKNMLSLLKASRPNDRAPLYCLN
                     QVGMPKRPEIGARGFAKTIESQPIAAIRFDPRLFGTAANNGQMIAELAAKHRTTEMFL
                     QIAQRLTGRGETKKPRGSFLAPIIRRLRAK"
     gene            complement(4757..5497)
                     /locus_tag="VGA15_01750"
     CDS             complement(4757..5497)
                     /locus_tag="VGA15_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEX9586456.1"
                     /translation="MTNRTPVDRYRTLRLLGALFGLSAALGACANSGGEVVTASVPDD
                     YRLRHPIAIQEADRSVVIFVGHARGGLSASQRADVAGLAQIWAREGTGAIVADVPVDT
                     PNARAAAATFREAQALLAAGGVPPRGIKLRHYHPDDPRAFATIRLSYPRIAAVAGPCG
                     LWPEDLGPSIANADYSENKSYHNFGCANQRNLAAMIDNPSDLEQPRSETPAYTTRRTE
                     AFEKYRKGDPTTTVYPEAEKAKLSDTGK"
     gene            complement(5535..7007)
                     /locus_tag="VGA15_01755"
     CDS             complement(5535..7007)
                     /locus_tag="VGA15_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX9586457.1"
                     /translation="MTCRGNQPVRRVIIAGALSFSAVAALAFNPALTPVLAAGDQAPI
                     PAVAGGSMKTRFLALGVGKSVVVDLPDIKDVLVADPKIANAVVHSGQRAYIIGAAVGQ
                     TNIVFFDSAGQQIAAYDIAIKRDLNGVRAALKQMLPNAGIQTEGVGDGVMLTGSVSSP
                     IEAQQAGELAARLVGGADKVVNSLVVRGRDQVMLKVTVAEVRRDIIKQMGVDLSASMN
                     YGTAVVNFNNSNPFTANNSPLVPGNNLTAAALTKAGLPSVTATLRAMESAGVVRTLAE
                     PNLTAISGESATFISGGEFPIPTGVTCQTSTTGAIGNCVQTVSFKKFGISLNFTPVVL
                     TEGRISLRVMTEVSEVSAENSLTGGAGGTTIPSIKTRRAETTLEIPSGGSIAMAGLIQ
                     EQTKQAVNGLPGLDSLPVLGALFRSQDFVNNQTELMVIVTPYIVRAVAQKELSRPDDG
                     FAPASDSQSTLLAQINRIYGVAARVEPVGSYQVSYGFIID"
     gene            complement(7025..7822)
                     /gene="cpaB"
                     /locus_tag="VGA15_01760"
     CDS             complement(7025..7822)
                     /gene="cpaB"
                     /locus_tag="VGA15_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX9586458.1"
                     /translation="MYTARIGVLILALSAGGVAAYLARGSDNTSPPPEPVVQLQTVDV
                     LVAKSDIGLGQSVNPENLQWQTWPAATASNHFIRRNERPEATKEVAGSIARAPFLAGE
                     PIREPKLVKANGSGFMAAILPTGMRAISTEISPETGAGGFILPNDRVDVILSKRDKNP
                     DKSGSADIVVSEIILTNVRVLAIDQAPKEKDGQNVVVGKTVTLELKPEQAETLARARQ
                     TGTLALALRSIADLNAVETGSDDQALRRGDGINVVRYGVSSPTTIQK"
     gene            8115..8615
                     /locus_tag="VGA15_01765"
     CDS             8115..8615
                     /locus_tag="VGA15_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586459.1"
                     /translation="MRFQVRSIVLLADFSHCREGVRLAPHNEIARADSSCFQRLTGSC
                     RKPPNAEDDQHLMMLQTATTLHQSLIPDQANFTVAHAREPAIPEVTFGVRQHSRPRLS
                     GACMVTRFSQFLTGAESWLLNLAWNAVSEGAAMFCSTEYTAMTDSRKLAHGRASSAFS
                     LRRSAL"
     gene            complement(8629..>8870)
                     /locus_tag="VGA15_01770"
     CDS             complement(8629..>8870)
                     /locus_tag="VGA15_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9586460.1"
                     /translation="DCVVRNISEQGASLEFSSAVKLPKGRISLTIAQKGRSFLAKIIW
                     WRDNFIGVAFSGGTPSALPVSDLEERLRKSEQKKR"
BASE COUNT         1726 a         2609 c         2794 g         1741 t
ORIGIN      
        1 gatacttact gcgtaaacct caacaagaag gcgcgcgacg gcaagatcga tccggtgatc
       61 ggacgaaatg ccgagatcaa tcgcgccatc caggtgctgt gccgccggca gaagaacaat
      121 ccgctgttcg tcggcgaagc cggcgtgggc aagaccgcga ttgcggaggg gctggcaaag
      181 cgcatcgtcg acagcgaagt tcctgaagtg ttggcggcgg cgacggtgtt ttcgctcgac
      241 atgggtacgt tgctggcggg cacgcgctat cgcggcgatt tcgaggagcg gctgaaacag
      301 gttctcaagg aactcgaggc gcatccgaac gccatcctgt tcatcgatga aatccacacg
      361 gtgatcgggg ctggcgcgac ttccggcggc gccatggacg cgtccaatct gctgaagccg
      421 gcgctcgcgt cgggcaccat ccgatgcatg ggctcaacca cttacaagga atatcgccag
      481 cattttgaga aggatcgcgc gctggtgcgc cggttccaga agatcgacat caacgagccg
      541 acggtcgagg acgcgattgc gatcctcaag ggtctgaagc cgtatttcga ggactaccac
      601 cggctgaaat acaccaacga ggccatcgag gccgcggtgc agctgtcgtc gcggtacatc
      661 catgaccgta aattgccgga caaggcgatc gacgtgatcg atgaatccgg cgcggcgcag
      721 atgctggtgg cggagaacaa gcgcaagaaa accatcggta tcaaggaaat cgagaccacg
      781 atcgcgacca tggcgcggat tccgcccaag agcgtgtcga aggacgacgc cgaggtgctc
      841 aagcatcttg agcagacgct caagcgcgtg gtgttcggcc aggacaaggc gatcgacgca
      901 ttgtcggcgt cgatcaagct ggcgcgggca gggttgcgcg aaccggaaaa gccgatcggc
      961 tcctatctgt tctcgggtcc gaccggcgtc ggcaagaccg aggtggcaaa gcagctcgca
     1021 gcttcgcttg gcgtcgaact gatccggttc gacatgtcag aatatatgga gcggcacacg
     1081 gtgtcgcgcc tgatcggcgc gcctccgggc tatgtcggtt tcgaccaggg tggcctgctc
     1141 accgatggcg tcgatcagca tccgcattgc gtggtgctgc tcgacgagat cgagaaggcg
     1201 catccggatc tgtataacgt gctgctgcag atcatggatc acggccggct gaccgaccat
     1261 aacggcaaac aggtcaattt ccgcaatgtg atcctgatca tgaccaccaa tgcgggcgcc
     1321 gccgatctcg cgcggcaggc ctttggcttc acgcgcaaca agcgggaagg cgacgatcac
     1381 gaagcgatca atcgccagtt cgcgcctgag ttccgcaacc ggctggacgc cattgtctcg
     1441 ttcgcgcatc tcaatgccga tgtgatcggc atggtggtgg agaagttcgt gctgcaactc
     1501 gaggcgcagc ttgctgaccg cgacgtcacc atcgaactgt cggagccggc caaggcgtgg
     1561 ctggtgcagc atggttatga tgagcagatg ggggcgcggc cgatggcgcg cgtgattcaa
     1621 gagcacatca agaagccgtt ggcggacgag gtgctgttcg gcaagctgaa gggcggtggc
     1681 cacgttcgcg tcgtgctggt gaaggacgag gcggccgcgg atgtggcgca ggaaaagatc
     1741 ggcttcgaat atgtcgaggg gccggtgacg ccgaagccgg aaaaactgcc cggcacgcgc
     1801 aagcgtaccg cgccacgcag gcagaaacca cccggcggtc ccagcggcgg ctccaagggc
     1861 ccggcctcac ggggtccgct ggtcaaggtg tgagcagctg atattgccaa caaaaaagcc
     1921 ggcggtcgca acaccgccgg cttttttgtt gggcttggct ttgatggctt ggctgggcgc
     1981 acgctgctgg cagcgtaacc ggattcacgg ccgttggaga tatcgccgag gaacccggta
     2041 aagggaattc aaaaagaata gatacattgc gacaggtccc gctaggcaga ttccaacgat
     2101 tctcctgcaa ggtggaatgc aggatggaca ctatcgagca gcgcgaagga tcgacatgtt
     2161 agcgaatcga cgtcagagcg agcgtcgcca ttgcagaagg gtcgcaaaga tccagttcgg
     2221 aaccggctcg ttgccgcgcg actgcacgat cacggacata tcggagggcg gcctgaaggt
     2281 aatcgccgaa tatcccgaaa ttcctcctca attcaccatc atattatcaa cgggccaccc
     2341 acgccaatgt cggctggcat ggcggatcgg ttgtgagttc ggcgctgaat tcatcgatta
     2401 gatcgaaaag ctctatcaaa acatcgaggc cggttaacgc tcttggtctg gaagcgcgaa
     2461 gtcgatatcg acgggctcgc tcaggttgca ccaaggcacc ccgcctgttt aactggaatt
     2521 tagggttaaa ctgcgaagca tttctggtca gtcgccgacg ccaggtcaaa gtacatgctc
     2581 cagcagttgc tccaacatta tcgcttcgcg aaacttcttg cgtccatagc atcgtccgcg
     2641 attttggtat tggggctttg tagctgccag actgcggcca cgtcggacat cacggggtcg
     2701 ctgggcgaga aaaccgaagc aagccgcggc gtcgaccctc ggcgcgaggt tgaatcttat
     2761 cgcgatcgct ttcgtgccaa ccccaaggac gccgaagcgg ccctacaata cgcaaaggcg
     2821 ctccgagccg cggggcagca ggcccaggcg gtcgcggtgc tcgaacaggc cacgatcgct
     2881 catcccggtg acaatgcgct gcttgccggc tacgggcgag cgctggcgga caatggcaat
     2941 ttccagcaag cattcgacgt cctcagccgc gcccatactc ccgacgatcc cgattggcgg
     3001 attctatcgg ctcagggaac cgcgctcgat caacttggcc ggcacgacga ggcccggcag
     3061 tactacgcga ccgcgctcaa gatcgcaccc gaagaacctt cagtgctgtc caatcttggc
     3121 ctttcgtatg ttctctcgaa agatctgccc aaagcggagg aagcgttgcg ccgcgcgtat
     3181 agccgcgcgg acgcggattc gcgtgtgcgg cagaatttgg cgttggtgat tgggctgcag
     3241 ggccgtcttg ccgaggctga aagcattgtg aaggccggtc ggccggccga ggaggcggcg
     3301 gcgaatgtcg cttatctgag gcgattgctg gctcgcaaag acaatgcaca cacggattcg
     3361 gataaaatac cggtggcagc catcggacgg ccgatctgac ctgcagcgaa cgagcctgcg
     3421 aatgagcctt gggctcggtc ggccgcgatc cgtcgcgtct tgcgaaacgc cgcggcaatc
     3481 ggcgcgacga cctactttgc tcgcagcctc ctgatgatcg gcgccaggaa cgagccccgc
     3541 ggctttttgg tctcgccgcg gcccgtcaac cgttgcgcga tctgcagaaa catctcggtg
     3601 gtgcgatgtt tggcggcgag ttccgcgatc atctggccgt tgttggccgc tgtgccgaac
     3661 agtcgcggat cgaatcgaat cgccgcgatc ggctggctct cgatcgtctt ggcgaatccg
     3721 cgcgcaccga tttccgggcg cttcggcatg ccgacctggt tgagacaata cagcggcgcc
     3781 cggtcgttgg gccgcgacgc tttcagcaag ctcaacatgt tcttggtatt gcgcagattg
     3841 gcgaggtccg gctcggcaac aatcaggata tcgtccgcac cgaccagagc gcgcttggtc
     3901 catcccgacc attggtgtgg aatgtcgagc acaatgcagg gcgttgtcat gcgtaacgta
     3961 tcgaacaccg agtcgaaggc attggcgccg aaatcataga cctgctcgag cgtcgccggc
     4021 gccgccagca ggctgaggtg ttcggtgcat ttcgacagca agcggtccat gtatgcggta
     4081 tcgagccgat cgggcgagaa caccgcgttg gcgatgccct gcaacggatc ctgattatag
     4141 tcgagaccgg cggtgccgaa tgcgaggtcg agatcgatga cgacggaatc cagcgcgaga
     4201 tcgcgcgcga tcgcccaggc gacgttgtgt gcgacggtgg atgcgccgac gccgcctttg
     4261 gcgccgacga ttgcgatgat ccggccgacg gctactgcct ccgatgccga gaacagactg
     4321 caaatcgagc gcaccacatc aagggtatcg accggcccaa cgacgtagtc gctgacgccg
     4381 cgacgcacca gttcgcgata aggcgcggca tcgttgatgc tgccgatcac gacgacgcgg
     4441 gttccggcgt cgcacacggt tgcgagttga tcgaggccgg ccagaatatc gctgcggccc
     4501 tcggtttcca ggatgatcac gttcggcgtg ggcaccgtgt gataggcttc gatggcggct
     4561 gccatgccgc ccatctgaat agagagatgg gccttgctga ggcggcggtc ttcgcccgcc
     4621 gatcgcacgg cggctgcggt ctccgcggtc tcgcaaaagg cctgtaccga tacacgaggc
     4681 gcaggagcga tgtaatcgtc cgcgggcgga ggtgcaatgt ccggctgctc ttcgggattt
     4741 tgacgggcga tgctgatcat ttgcctgtgt cgctgagttt ggccttctcg gcttcgggat
     4801 agacggtcgt ggtcgggtcg cccttgcgat acttctcgaa tgcttccgtg cgtcgcgtcg
     4861 tatacgcagg tgtctcggac cgcggctgct cgaggtccga cggattgtcg atcatcgctg
     4921 cgaggttgcg ctggttagcg caaccaaaat tgtggtacga cttgttttcg gaatagtcag
     4981 cgttcgcaat cgagggtccg agatcttccg gccacagacc gcagggtccg gcaaccgcgg
     5041 caatccttgg ataactcagc ctgatcgtcg cgaacgctcg agggtcgtcc ggatggtagt
     5101 gacgcaactt aattccgcgt ggaggcacgc cgcctgccgc caatagcgcc tgagcttcgc
     5161 ggaatgtcgc cgccgcagcc cgtgcgtttg gcgtgtcgac cggcacgtcc gccacgatcg
     5221 cgccggttcc ttcgcgagcc catatctgtg ccagtcccgc gacgtcggcg cgctgtgaag
     5281 cggacaggcc gccacgtgca tgaccgacga aaatcacgac tgagcggtcg gcttcctgaa
     5341 tcgcgatcgg atgacgcagg cggtaatcgt cgggaacgct tgccgtcacc acttctccac
     5401 cgctattggc gcaggcaccc agcgcggccg aaaggccaaa cagtgcgccg agcaggcgca
     5461 gggttcgata gcgatcgacg ggcgttctgt ttgtcatgtg ctgtcctcgt cccgtattgg
     5521 tgggcagttc cgtctcaatc aatgataaag ccgtagctga cctggtagct tccaaccggt
     5581 tcgacacgag cggcaacgcc gtaaattcga ttgatctggg ccaacaatgt cgattgggaa
     5641 tccgacgccg gcgcaaaccc gtcatcggga cgcgacagct ctttttgggc gaccgcccga
     5701 acgatgtagg gcgtcacgat gaccatcagc tcggtctggt tattgacgaa gtcctggctc
     5761 ctgaaaagcg ctccgagaac cggcaatgag tcgaggccgg gcaatccgtt gacggcctgc
     5821 ttggtctgtt cctggatcag gcccgccatc gctatcgagc cgccggacgg gatttccagc
     5881 gtggtttcag cgcgacgggt cttgatcgag ggaatcgtcg ttccgccggc accgccggtc
     5941 aaagagtttt cggccgagac ttccgacact tcggtcatca cccgcaggct gatccgtccc
     6001 tcggtcagca ccaccggcgt gaagttgagc gagatgccga atttcttgaa actgaccgtc
     6061 tggacgcaat ttccgatggc gcccgtcgtc gacgtttggc aggtcacacc ggtcggaatt
     6121 ggaaattcgc cgcctgaaat aaatgtcgcg gactcgccgg agattgccgt cagatttggc
     6181 tctgcaagcg tccgcaccac gcccgcgctc tccatcgcgc gcagggtagc ggtgaccgac
     6241 ggcagaccag ctttggtcaa ggccgccgcg gtcaagttgt tgccagggac gagcggtgaa
     6301 ttgtttgcgg tgaagggatt gctattgttg aagttcacga ccgcggtgcc gtaattcatg
     6361 ctggcgctga gatcgacgcc catctgtttg atgatgtccc gccgcacttc cgcgacggtg
     6421 accttcaaca tcacctgatc gcggccacgg accacgaggg agttgacgac cttgtcggca
     6481 ccgcccacca gtcgggcggc aagttcgccc gcctgctgcg cctcgatcgg actcgatacc
     6541 gagccggtca gcattacgcc gtcgccgacg ccttcggtct ggattcccgc gttgggcagc
     6601 atttgcttca acgcggcgcg cacgccgttg agatcgcgct tgatcgcgat gtcataggcc
     6661 gcgatctgct ggcccgccga gtcgaaaaag acgatgttgg tctgaccgac cgccgcgccg
     6721 atgatgtagg cgcgttgccc cgagtggacg accgcgttgg caatcttggg gtcggcgacc
     6781 agcacatcct tgatgtcggg cagatcgacg acgaccgatt tgccgacgcc aagcgcaaga
     6841 aagcgtgtct tcattgatcc gccggcgaca gccgggattg gcgcttggtc gcctgccgcc
     6901 agaaccggcg tcaacgccgg attgaacgcc agcgccgcaa cggccgaaaa cgacagggcg
     6961 ccggctataa ttacgcgcct caccggctga tttcccctgc aggtcatgtc gggtgtcctt
     7021 ttcatcactt ctgtatcgtc gtcgggcttg agacgccgta gcgaacgaca ttgatgccgt
     7081 cgccacgcct gagcgcctga tcgtcggatc cggtctcgac cgcgttgaga tcggcaatgc
     7141 tgcgcagcgc cagcgccagc gtgccggtct ggcgcgcgcg ggcaagcgtc tcggcttgct
     7201 cgggcttcaa ttcgagcgtg acggtcttgc cgaccacgac gttctggccg tctttttcct
     7261 tcggagcctg gtcgatcgcg agcacgcgaa cattggtcag gatgatttca gagacgacga
     7321 tatccgctga acccgatttg tccgggttct tgtcgcgctt cgaaagaatc acatcgaccc
     7381 ggtcgttcgg caggatgaag ccgccggcgc cggtctcggg cgagatttcg gtcgagatgg
     7441 cgcgcatgcc ggtgggcagg atcgccgcca tgaagcccga cccattggcc ttgaccagct
     7501 tcggctcccg gatcggctcg cccgcgagga atggcgcacg cgcaatcgag cccgcgacct
     7561 ctttggtggc ttcgggccgt tcgttgcggc ggataaaatg attgctcgca gttgcggccg
     7621 gccacgtctg ccactggaga ttttccgggt taaccgactg gccgaggccg atgtcggact
     7681 tcgccaccag aacatccacc gtctgcaact gtacaactgg ctcgggcggg ggagatgtgt
     7741 tgtcagaccc acgcgccaga tatgcggcaa cgccgccggc actgagagcg aggatcagga
     7801 cgccaatacg tgcggtatac atacgcttta ctatctactt ttacgccggg atccctgaac
     7861 tgcatggtgg tgacagcctt tcccctacgc atcactaagc agcaaaggta taagccaact
     7921 tgaggtcgaa agaatttgca gaaaggaatt gagggatttg tgctcgtggt gaacggagcg
     7981 ttcatggtga agacagcgtt atcgtccagc aagtgaaccc gcaaaaatcc cgacatgatc
     8041 gatgttgata ccgagagtct gccgcagggg gatagcgacc ccgggctcga ctcggtgtct
     8101 gctggatccc tggaatgcgt ttccaggttc gctcaattgt tcttttggcc gatttttctc
     8161 attgccggga aggcgttcgg cttgctccgc acaacgaaat cgcgcgcgcg gattccagct
     8221 gttttcagag attgacggga tcttgccgca agccaccgaa tgctgaagac gatcagcacc
     8281 tcatgatgct tcagaccgcg accacccttc accagtccct gatccctgat caggcaaact
     8341 tcaccgttgc tcatgcgcgc gagccggcaa ttcctgaagt aacttttggc gtaagacagc
     8401 attcgcgccc ccggttgtca ggagcatgca tggtcacacg attcagtcaa ttcttgaccg
     8461 gcgccgaatc ctggttgctg aatttggcat ggaatgccgt atcggagggc gccgccatgt
     8521 tttgctccac cgaatacacc gcgatgacgg actcccgcaa gcttgcgcat ggtcgggcgt
     8581 cgtccgcatt cagtcttcgc cgatcagctc tttgatgcgg cgctgcagtt agcgtttttt
     8641 ctgctcgctc ttgcgcagcc gctcctcgag atcggagacg gggagtgcgg atggggtccc
     8701 cccgctgaac gcaacgccga tgaaattgtc gcgccaccag atgattttgg cgaggaagga
     8761 acggcctttt tgcgcgatgg tcagcgatat ccgccctttg ggcagcttga cggcgctgct
     8821 gaattcgagg ctggcgcctt gttcggaaat gttgcggaca acgcagtctg
//
LOCUS       DASWGM010000052         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2082705,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000052 DASWGM010000000
VERSION     DASWGM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2082705"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VGA15_01775"
     CDS             complement(<1..950)
                     /locus_tag="VGA15_01775"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX9586461.1"
                     /translation="MQRTKAPFRADEVGSLLRPPKIKEARAKLENGEISAADLRKVED
                     MEIEKVVHKQASIGLKLATDGEFRRSWWHFDFLSHLTGCELFHPDEGIQFAGIQTRHD
                     AVRVIGKLDFPDDHPMLEHFKFLKKHCEIAHVVPKMTIPSPAVLHFRGGRKSISKEVY
                     PDIEVFFEDLAKTYRKAVKAFYDAGCRYLQLDDTVWAYLCSQDELDKVRARGESADNL
                     QEIYARIINYAIAERPADMTITTHVCRGNFRSTWISSGGYEPVAETMLAGTSYDGYFL
                     EYDSERAGGFEPLRYLPKGNKIVVVGVITSKTGQLENKDD"
     gene            complement(968..1684)
                     /locus_tag="VGA15_01780"
     CDS             complement(968..1684)
                     /locus_tag="VGA15_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586462.1"
                     /translation="MPDLLRIEGLRAGYGEAVVLPDLSLHLPESQVLALLGRNGTGKT
                     TLINSIVGVNKRFGGTINLGGLDITPMRPDQRARAGIGWVPQERNIFRSLTVEENMTA
                     VAQPGPWTVAKVYEMFPRLKERRNNFGNQLSGGEQQMLAIGRALTLNPRVLLLDEPTE
                     GLAPIIVEELLRALGTITRAGGTCSIIVEQNARKILGLADRVVILERGVIVHDAASAA
                     LKADPAVLERFLGVSGAAAH"
     gene            complement(1677..2429)
                     /locus_tag="VGA15_01785"
     CDS             complement(1677..2429)
                     /locus_tag="VGA15_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586463.1"
                     /translation="MTTALQTTGLAKQFGGLKVTRDLSLKIEQGARHALIGPNGAGKT
                     TVINLLTGVLKPNAGRILLEGNDITDLPVHARVLRGLSRTFQINQLYADLSPLETIGL
                     AVSERMGHGGDWWRRMGTRDDVNAEIAENLARFRLLEVMNEPTATLPYGKQRLLEIAV
                     AIAAKPRVLLLDEPAAGVPESERHDILAAVAALPRDVTVLLIEHDMDLVFSFADRISV
                     LVNGALLVEGPPDEVARDARVKAVYLGEAADA"
     gene            complement(2426..>3310)
                     /locus_tag="VGA15_01790"
     CDS             complement(2426..>3310)
                     /locus_tag="VGA15_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9586464.1"
                     /translation="LFALSLDLILGYAGIVSLGHAAFFGVGAYSAGLLALHGVVTEPV
                     VALVVAGLVATVVGFLTSFLVIRGVDLTRLMVTLGIALLLEALAERFSNITGGTDGLQ
                     GIEMQPILGLFAFDMFGKVGFFYSLAVLFVLLLLARRIVHSPFGLSLRAIRNNPLRAA
                     AIGIPVNRRLIAIYTLAAFYAGIAGALFTQTTALASLDVFSFERSADLMLVLVIGGTG
                     YLYGGLIGAVVFKMLQEFFSNLTPQYWQFWIGLVLVVIVLVGRARMHRWALWVPNLII
                     RQLGGRKAIVAVPESDAS"
BASE COUNT          669 a         1065 c          998 g          578 t
ORIGIN      
        1 atatcgtcct tattctcgag ttggccagtc ttcgaggtga tgacgccgac cacgacaatc
       61 ttgttgcctt tcggcaaata ccgcagcggc tcgaagccgc cggcccgctc ggaatcgtat
      121 tcgagaaaat agccgtcgta actggtgccg gccagcatgg tctccgccac cggctcataa
      181 ccgcccgacg aaatccaggt agagcggaaa ttgccgcgac aaacatgcgt ggtgatcgtc
      241 atgtccgcag gccgttcggc gatcgcgtaa ttgatgatgc gggcgtaaat ttcctgcagg
      301 ttgtcggcgc tctcgccgcg cgctcgcacc ttgtcgagtt catcctgcga gcagagatag
      361 gcccataccg tgtcgtcgag ttgcagatag cggcaaccgg cgtcgtagaa cgcctttacc
      421 gccttgcgat aggtcttggc gaggtcctcg aaaaacacct cgatgtcggg atagacttcc
      481 ttcgagatcg acttgcggcc gccgcggaaa tgcagcaccg cgggtgacgg gatcgtcatc
      541 ttcggcacca catgcgcgat ctcgcaatgc ttcttcagga acttgaagtg ctcaagcatc
      601 ggatgatcgt ccggaaaatc gagcttgccg atcacacgca ccgcgtcatg ccgggtctgg
      661 atgcccgcaa actggatgcc ctcgtcggga tgaaacaatt cgcagcccgt aagatggcta
      721 agaaagtcaa aatgccacca cgagcggcgg aattcaccat cggtcgcgag cttcaggccg
      781 atcgatgcct gcttgtgcac gaccttttcg atctccatgt cttcgacctt gcgcagatcc
      841 gcggccgaaa tctcgccgtt ctccagcttg gcgcgcgcct ccttgatctt cggcggccgc
      901 aacaggctgc cgacttcgtc ggcgcggaaa ggggctttgg ttcgttgcat ggctcactcc
      961 cgaggcttta atgtgccgca gcccccgaga cgccgagaaa gcgttcgagt accgcgggat
     1021 cggctttcaa ggcggcgctg gccgcatcat ggacgatcac gccgcgctcc aatatcacaa
     1081 cgcggtcggc cagccctaga atctttcggg cattctgctc gacaatgatc gagcaggtgc
     1141 cgccggcgcg ggtgatggtg ccgagcgccc tgagcaattc ctcgacgatg atcggagcaa
     1201 gcccctcggt cggctcgtcc aatagcagca cccgcggatt gagcgtgagc gcgcgaccga
     1261 tcgcgagcat ctgctgctcg ccgccggaca gctgattgcc gaaattgttg cggcgttctt
     1321 tgagccgcgg aaacatttcg taaaccttgg cgaccgtcca cggtcccggc tgcgccaccg
     1381 cggtcatgtt ttcttcgacc gtcagcgatc ggaagatgtt gcgctcctgc ggcacccagc
     1441 caatacccgc ccgggcccgc tggtccggcc gcatcggcgt gatatccagc ccgccaagat
     1501 tgatggttcc gccaaaccgt ttgttaaccc cgacaatcga attgatcagc gtggtcttgc
     1561 cggtgccgtt gcgacccaac agcgccagca cctggctttc cggcagatgc aacgacagat
     1621 cgggcaatac caccgcttcg ccgtagcccg cgcgcagacc ttcgatacgc aacagatcag
     1681 gcatcggcgg cctcgccgag atagacagcc ttgactctgg cgtcgcgcgc cacctcgtcc
     1741 ggtgggcctt ccaccagcag cgcgccattg accagcaccg aaatccgatc ggcgaacgag
     1801 aacaccagat ccatgtcgtg ctcgatcagc agcacggtga cgtcgcgcgg caacgctgcc
     1861 acggcggcaa gaatatcgtg gcgttcgctt tcgggaacgc cggcggcggg ctcgtcgagc
     1921 agcagcacgc gcggcttggc ggcaatggcg accgcaatct ccagcaggcg ctgttttcca
     1981 taaggcaacg tcgccgtcgg ttcgttcatc acctcgagca gccggaagcg ggccagattt
     2041 tccgcgatct cggcgttgac atcatcgcgg gtgcccattc gccgccacca gtcgccgcca
     2101 tggcccatgc gctccgacac cgccagaccg atggtctcga gcgggctcag atcggcataa
     2161 agctggttga tctggaaggt tcgcgacagc ccgcgcagca cccgcgcatg caccggcaaa
     2221 tccgtgatgt cgttgccctc aagcagaatg cgcccggcgt tgggcttgag aacgccggtc
     2281 aacaggttga tgacggtggt cttgccggcg ccattcggac cgatcagcgc atggcgggcg
     2341 ccctgttcga tcttcaacga caggtcgcgg gtgactttaa gtccgccgaa ctgctttgcc
     2401 agtcccgtcg tttgcagcgc ggtcgtcatg acgcgtcact ctcgggaacg gcaacgattg
     2461 ccttgcgccc gccgagttgc ctgatgatca ggttgggtac ccacaatgcc cagcggtgca
     2521 tgcgtgcgcg gccgaccagc acgatcacaa cgagcacgag gccgatccag aactgccagt
     2581 attgcggcgt gagattggaa aaaaactcct gcagcatttt gaacaccacc gcgccgatca
     2641 atccgccata gagatagccg gtgccgccga tcaccagcac cagcatcaga tcggccgagc
     2701 gctcgaacga gaacacgtcg agcgacgcca gcgccgtggt ctgggtgaac agcgcgccgg
     2761 cgataccggc gtaaaacgcc gccagcgtat aaatcgcgat caggcgccgg ttgacgggga
     2821 tgccgatcgc ggcggcacgc agcggattat tgcggatcgc gcgcagcgac agaccgaacg
     2881 gcgagtgcac aatccgccgc gccagcagca acagcacgaa caacacggcg agggaataga
     2941 agaagccgac cttgccgaac atgtcgaacg caaacaggcc gaggatcggc tgcatctcga
     3001 tgccttgcag tccgtcggtg ccgccggtga tgttggaaaa ccgctccgcc agcgcttcca
     3061 gcaggagcgc gatccccagc gtcaccatca ggcgggtcag gtcgacgccc ctgatcacca
     3121 ggaaactggt gagaaagcca actacggtgg cgacgaggcc ggcaaccacc agcgcgacca
     3181 cgggctcggt gacgacgccg tgcaacgcca agagcccggc ggaataggcg ccgacgccga
     3241 agaacgcggc gtgaccgagc gagacgatgc cggcgtagcc caggatcaga tcgagcgaca
     3301 gcgcgaacaa
//
LOCUS       DASWGM010000053         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1806800,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000053 DASWGM010000000
VERSION     DASWGM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806800"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VGA15_01795"
     CDS             <1..298
                     /locus_tag="VGA15_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX9586465.1"
                     /translation="KGMLKNADGTPMKKEDFRIIHKSAQIINGPYAYSSELPEDLKAA
                     IANAFAEAPTKDKAAFDRLSDGQKKGFHPATTKDWDATIELIKFVDNLRKKKAS"
     gene            377..1261
                     /gene="phnE"
                     /locus_tag="VGA15_01800"
     CDS             377..1261
                     /gene="phnE"
                     /locus_tag="VGA15_01800"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX9586466.1"
                     /translation="MTVAVSILPEQQLAVLNNAYRKAVARERLRLTLATAVFLAALVI
                     AAIGAEVNLRTFFTYFGNFVSYFDRILTLENGARVWTDIGEWFWGWRKWLWMLGETIL
                     ISYVGTLFGAILGFSLNFFAAENTSPAPWLRFVVRRVLEFARTVPGIVFALIFVIAFG
                     LGPMAGVLAIAIHSTGALGKLFSEIVENADMKPVEGVRSTGASWISCMRFAVLPQVAA
                     GYASYALLRFEINVREASVMGFVGAGGIGQELVVAIRKFYYSDVSAILLTIIVTVFVI
                     DIGTGWMRSRLFGKDARR"
     gene            1258..2106
                     /gene="phnE"
                     /locus_tag="VGA15_01805"
     CDS             1258..2106
                     /gene="phnE"
                     /locus_tag="VGA15_01805"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX9586467.1"
                     /translation="MTQLPAPDRETLRARYPLVFNRPVSARLAMPAMLLGAFGIFAFG
                     LVDLDFSPARLVSGLHQLGWITLMMVPPDPGSSLPAYLVALGETLSIALLGTTIAAVF
                     ALPVSLLAAKNIVPSNIFRFPVRRFLDSIRGVDTLIWALVWINVVGLGPFAGVLAIAV
                     SDFGAFGKLFSEAIEAADSKQVEGIRASGGNALHEIRFGLMPQVLPVIAGQVLYFIES
                     NTRSATIIGIVGAGGIGLQLAEQIRVLEWQKVSFLILMILVAVAVIDWISGKLRFAII
                     GRRAVA"
     gene            complement(2117..2845)
                     /gene="phnF"
                     /locus_tag="VGA15_01810"
     CDS             complement(2117..2845)
                     /gene="phnF"
                     /locus_tag="VGA15_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HEX9586468.1"
                     /translation="MSMQGAASGVALWRQVADGIERGIADGRFAAGDRLPGETEIAEA
                     YRVNRHTVRRALATLAERGLVRAERGSGTYVEAPRLAYPLRSRTRFSEIAGAGGREPD
                     GQLIGASGELASRELARLLGLKTGAPLVRIEALRLADRTPICVGTIWFAAERFPDAGL
                     IYERVRSMTKLLAHYGIRDFRRASTRVTAAIVDATDAARLDLALGRPVLVVDSTDVDT
                     EGKPLMTKRTRFAAERIEFVVENG"
     gene            2964..3425
                     /gene="phnG"
                     /locus_tag="VGA15_01815"
     CDS             2964..3425
                     /gene="phnG"
                     /locus_tag="VGA15_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HEX9586469.1"
                     /translation="MTVTRENSKQAQRKAAMTVLAHSDAAGIASRLDVLALPDHEILR
                     EPENGLVMVRGRIGGDGAPFNLGEATVARAAVRLSTGEVGFGYTLGRDRQKARMIALC
                     DALIQSNEFAEAVEAEVIAPLRAQMISERDRRAAETAATRVDFYTLVRGEG"
     gene            3429..4037
                     /gene="phnH"
                     /locus_tag="VGA15_01820"
     CDS             3429..4037
                     /gene="phnH"
                     /locus_tag="VGA15_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEX9586470.1"
                     /translation="MTTVAELPAGFADKVLSAQSTFRSVMAAMARPSSVQRIAAAAGT
                     PAAMMRGTAAIALTLFDHDTPLWLDPLMSETSEVTKWLKFHTGAPVIADSSICSFALI
                     GDARALPSLDRFAFGSSEYPDRSTTLILQVESLTQGSVLELRGPGIDGVAMLQATIQP
                     TDLFERLAINAALFPRGIDVVLVHDDAIVAIPRTTRLVAKGD"
     gene            4040..>4720
                     /locus_tag="VGA15_01825"
     CDS             4040..>4720
                     /locus_tag="VGA15_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HEX9586471.1"
                     /translation="MYVAVKGGERAIENAHRLLAHERRGDRDFPEISLDQISGQLSLG
                     VDRVMTEGSLYDRELAALAIKQARGDMIEAIFLLRAFRATLPRFGATEPVDTGRMQVR
                     RRISSTFKDIPGGQVLGPTFDYTHRLLDSQLAEGLVPEPPATAEASPATMPRVTDILG
                     RDGLIEPSPEADADAPVGDLTREPLSFPADRDLRLQNLARADEGFVLALGYSSQRGYG
                     RNHPFAGEI"
BASE COUNT          814 a         1465 c         1499 g          942 t
ORIGIN      
        1 caaggggatg ctgaagaacg ccgacggcac gccgatgaag aaggaggatt tccggatcat
       61 tcacaaatcg gcgcagatca tcaacggacc ctatgcctat agttcggagc ttccggaaga
      121 tttgaaggcc gccatcgcca acgcattcgc cgaggcgccg accaaggaca aggccgcttt
      181 cgaccggctg tccgacggcc agaagaaggg tttccacccg gcgacgacca aggattggga
      241 cgctacgatc gaactgatca agttcgtcga caatcttcgc aagaaaaagg cgtcctgagc
      301 gtcaccgaga ctagataggc cgggtctgca cgacccggct tttttccgtt cagattttgc
      361 cagagcagca gcttcaatga ccgtcgccgt ttccatcctt ccggagcagc agctggcggt
      421 gctgaacaat gcctatcgca aggcggtcgc gcgcgagcgc ctccggctta ccttggcaac
      481 ggcggtgttt cttgccgcat tggtcatcgc cgccataggc gcggaggtga acttaaggac
      541 tttcttcacg tattttggca acttcgtcag ctatttcgat cgcatcttga cactggaaaa
      601 tggcgccagg gtctggaccg acatcggcga atggttctgg ggttggcgca aatggctttg
      661 gatgctcggc gagactatcc tgatcagcta cgtcggaacc ttgttcggcg cgatccttgg
      721 cttctcgctc aattttttcg cggctgaaaa tacctcgccg gcgccgtggt tgcgcttcgt
      781 cgtgcgccgc gtcctggaat tcgcccggac agtccccggc attgtgttcg cgcttatttt
      841 tgtcatcgcg ttcgggctgg gaccgatggc aggtgtgctg gcgattgcga tccattcgac
      901 cggcgcgctc ggaaaactgt tctccgagat cgtcgagaat gccgacatga aaccggtcga
      961 aggggtgcgg tcgacgggtg cgagctggat atcctgcatg cgttttgccg tgttgccgca
     1021 ggtggcggcg ggctacgcca gctacgcttt gctgcgcttc gagatcaatg tccgggaggc
     1081 gtcggtgatg ggcttcgtcg gtgccggcgg catcggccag gaactcgtcg tcgccatccg
     1141 gaaattctac tattccgacg tcagcgcgat cctgctgacg atcatcgtca cggtctttgt
     1201 catcgatatc ggcaccggct ggatgcgtag ccggttgttc ggcaaggatg cacgaagatg
     1261 acccagttgc cggcaccgga tcgggaaact ctgcgggcgc ggtaccccct ggtgttcaac
     1321 aggccggtct cggcgcgact ggcgatgccg gccatgttgc ttggcgcttt cggcattttt
     1381 gcattcgggc tggtcgatct cgacttttcg ccggctcggc tcgtctccgg cctgcatcag
     1441 cttggctgga tcactctgat gatggtcccg cccgatccgg gctcctcgct tcctgcctat
     1501 ctggtcgccc ttggagagac cttgtcgatc gcgctgcttg gcaccaccat agcggccgtc
     1561 ttcgcgctcc cggtcagtct gctggcggct aagaacatcg taccgtccaa tattttccgc
     1621 tttccggtcc gccggtttct cgattcgatt cgcggcgtcg atacgctgat ctgggcgttg
     1681 gtctggataa atgtcgttgg tctcggcccg tttgccggcg tgctggcgat tgcggtgtcg
     1741 gatttcggtg cgttcggcaa gctgttctcc gaggcaatcg aggccgccga cagcaagcag
     1801 gtcgaaggca tccgggcatc tggtggcaat gcgctgcacg aaatacgctt tggcttgatg
     1861 ccccaggtgc tgcccgtcat tgccgggcag gtgctctatt tcatcgagtc gaacacgcgc
     1921 tcggcgacca tcatcggcat tgtcggggcc ggcggcatcg gtctgcaact ggccgagcag
     1981 atccgtgtgc tggaatggca gaaggtgtcg ttcctcattc tcatgattct agtggcggtt
     2041 gccgtgatcg actggatttc gggaaaactt cgctttgcca tcatcgggcg gcgcgcggtc
     2101 gcatgaagat cgcgagctaa ccgttctcaa cgacgaactc gatcctttcc gcggcaaagc
     2161 gcgtgcgctt ggtcatgagt ggtttgcctt cggtatcgac gtcggtgcta tcgaccacca
     2221 gcacggggcg ccccagcgca aggtcgagcc gtgccgcatc ggtggcatcg acaatggccg
     2281 ccgtgacgcg ggtcgacgcc cggcgaaaat cgcggattcc gtaatgcgcc agcagttttg
     2341 tcatcgaacg cacacgctca tagatcagcc cggcgtcggg aaagcgttcc gcggcgaacc
     2401 agatggtgcc gacgcatatc ggcgtgcgat cggccagccg cagtgcctcg atccggacca
     2461 gcggggcgcc tgtcttcaag cccagcaggc gcgccagttc gcggctcgca agctccccgg
     2521 aggcgccgat caactggccg tccggctcgc ggccaccggc gccggcaatc tcggaaaatc
     2581 gcgtgcgtga acgcagcggg taggcaagcc gtggcgcctc cacataggta ccgctgccgc
     2641 gttcggcgcg taccaggccg cgctcggcca gtgtcgccag cgccctccgc accgtgtgcc
     2701 ggttaacccg ataggcttct gcgatttcag tctcgcccgg cagccgatcg cctgcggcaa
     2761 agcggccatc ggcgataccg cgctcgattc cgtcggcgac ctgccgccaa agtgccacgc
     2821 cggaggccgc gccctgcatg ctcatcgggt cgaaaggccg gaaaaagcca tcggaaaaat
     2881 catcctgttg tcacgaaaca gtcatggcgc ttcgctacca agttgtctaa tatcatagac
     2941 aacttgattc cggcaaggcc gatatgaccg tgacgcgcga aaacagcaaa caggcgcagc
     3001 gaaaagcggc gatgaccgtg ctggcgcatt ccgatgcggc cgggattgcc agccggctcg
     3061 acgtgctcgc actgccggac cacgaaattc tgcgcgagcc ggaaaacggt ctggtgatgg
     3121 tgcgcggccg catcggcggc gacggcgcgc cgttcaatct cggcgaagcc acggtcgcgc
     3181 gcgcggcggt gcggctgtcg accggcgagg tcggctttgg ctacacgctc ggccgcgacc
     3241 ggcagaaggc gcggatgatc gcgctctgcg acgcgttgat ccagtcgaac gaatttgccg
     3301 aggcggtaga ggcagaagtg attgcaccgc tgcgtgcgca gatgatctct gaacgagacc
     3361 gaagggctgc ggaaaccgcg gcgacacggg tcgatttcta cacgctggta cgcggtgagg
     3421 gctgaacgat gacgacggtt gccgaactgc cagcagggtt tgcggacaag gtgttgtcgg
     3481 cgcaatcgac gttccgttcg gtcatggccg cgatggcgcg ccccagcagt gtgcagcgca
     3541 tcgcggccgc cgccggcacg cccgccgcga tgatgcgcgg caccgcggcg atcgcgctga
     3601 cattgttcga tcatgacaca ccgctctggc tcgacccgtt gatgtccgag acatcggagg
     3661 tgacaaaatg gctcaagttt cataccggtg cgcctgtcat cgcggattcc tcgatctgca
     3721 gttttgccct gatcggcgac gcgcgcgcgc ttcccagcct cgatcgcttt gctttcggca
     3781 gcagcgaata tcccgatcgc tcgacgacgc tgatcctgca ggtcgaaagc ctgacgcaag
     3841 gttccgtgct cgaattgcgc gggcccggca tcgatggcgt cgcgatgttg caggccacca
     3901 tccagccgac ggacctgttc gaacggcttg ccatcaacgc ggcgctgttt ccgcgcggca
     3961 tcgatgtcgt gctggtccat gatgatgcga ttgttgcgat accgcggacc acgcggctgg
     4021 tcgcgaaggg agactgagca tgtatgtcgc cgtcaagggt ggcgaacgcg ccatcgagaa
     4081 cgcccatcgc ctcttggcgc atgaacggcg tggcgaccgc gacttccctg aaatctcgct
     4141 ggatcagatt tccgggcaac tcagcctcgg tgttgatcgc gtcatgactg aaggctcgct
     4201 gtacgatcgc gaactggcgg cgctggccat caagcaggcg cgcggcgaca tgatcgaggc
     4261 cattttcctg ctgcgcgcat tccgcgcgac attgccgcgg ttcggtgcga ccgagccggt
     4321 cgataccggc cggatgcagg tgcgccgccg gatttcctcg accttcaagg atattccggg
     4381 cggacaggtg ctcgggccta ccttcgatta cacacaccgt ctgctggatt cccaacttgc
     4441 cgagggtctt gtcccggagc cgccggcaac cgccgaagca tcgcccgcga cgatgccgcg
     4501 cgtcaccgac atactcggcc gcgacgggct aatcgagccg tcgcccgagg cggacgccga
     4561 tgcaccggtg ggcgacctca cccgcgagcc gctgagcttt cccgccgacc gcgacctgcg
     4621 gctgcagaat ctcgcgcgcg ccgacgaagg ctttgtgctg gcgctcggct attcgtcgca
     4681 gcgcggctat ggccgcaacc acccgttcgc cggcgagatc
//
LOCUS       DASWGM010000054         5584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1112296,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000054 DASWGM010000000
VERSION     DASWGM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112296"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VGA15_01830"
     CDS             <1..804
                     /locus_tag="VGA15_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586472.1"
                     /translation="WNDPKDFPWTMGWQPNYQSESQIYAKYLLKQKPDAKIAVLYQND
                     DYGKDYLKGLKDGLGAKAATMIIAEESFETTEPTIDNHIVKLKSTGADVFVNITTPKF
                     AAQAIKKVAEIEWKPLHILNNVAASVGSVIKPAGYENAQGIISAAYLKDASDPQWDND
                     PGMKEFLAFLAKDFPEGNKLDLSVVVGYGVSQTLVKVLQMCGDNLTRENIMKQAANLK
                     DFRTEVLLPGIKINTGPDDFAPISQLQLMKFNGEKWDLFGDVISADVGG"
     gene            1062..2348
                     /locus_tag="VGA15_01835"
     CDS             1062..2348
                     /locus_tag="VGA15_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX9586473.1"
                     /translation="MTDRRPLLRTIFDAAVAAAHPDVVLAAHLRPVPKGRVICLAAGK
                     AAAAMAAAAERYYLDELGLEPARLLGIATTRHGHGVPTRRIRVVEAGHPVPDEAGMKG
                     AEDSLRLAQSAGADDLLLVLLSGGGSANWTAPAEGVSFAQKRQLTRALLRSGAPIGEV
                     NTVRKHLSRIKGGRLARAGKLAEIVTLAISDVPHDDPSAIASGPTVPDSTTLADARAL
                     VAKYGFKVDEAIRDALNDAKNESCKPGDAAFARAQFELIARPKASIDAAIRLARDAGY
                     EIIDLGADLEGEVRNVAADHAQLALRARAQGKRVAIVSGGELTVTVRGNGRGGPNQEY
                     ALALAGLLKDTSGIAALAGDTDGADGGAGKATDPAGAMIDQNTFAKMTSLGLDPGAYL
                     DNNDATAFFAATGDLLLTGPTLTNVNDIRVILVDGT"
     gene            2611..4761
                     /locus_tag="VGA15_01840"
     CDS             2611..4761
                     /locus_tag="VGA15_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9586474.1"
                     /translation="MTGSMELAIDPKPAHRTWLLEEVINNLSLGLIVFDNKREVVFCN
                     KRYMEIYGLSSEQVKPGTPTSELIQHRLNLGLKVPSKPDDYIRERVANAVVPGATVHE
                     FTDGRIIAYTVYPMPDGGGMTTHEDITGREELSARLKMLYELGRQQEENLRIRNLQFD
                     TAINNMSQGLCFFDAAQRLIVCNDRYVEMYGLPPDRVGPGTPLADIVDMRFEAGSFPA
                     MSRDEYLHWRSKIAVSAKPTESIVELRNGRTFKIRHRPMPDLGWVATHEDITEQRRSE
                     VKIEYMAHHDSLTGLANRVLLNERLEDAIGRINREEMVAVHHLDLDQFKAVNDTFGHP
                     VGDKLLKIVADRLRGLVRETDTIARMGGDEFVIVQPFIADPAEATALAHRVIKLMSEP
                     YGIDGHQAVIGASIGIAVGPGDGSRADKLLRNADLALYRAKGDGRGTFRFFEPAMDLQ
                     MLTRRIMEQDLRKALPAGEFELYYQPVVNLANSVISGFEALIRWNHPERGIVSPAAFI
                     PLAEEIGFIVPLGEWVIRQACATAARWPEDLRVAVNISAVQFRNPGLMQVIVGALAAS
                     GLDPTRLEIEITETVLLHNREATLAVLHQLRALGIRIAMDDFGTGYSSLTYLQCFPFD
                     KIKIDRSFVKDITENAGSLNIVRAVAALANGMGMTATAEGVETREQLDQITSEGCTEM
                     QGFLFSQPIRAHEIERLFLSEHEARKGLGGTVAA"
     gene            complement(4852..5367)
                     /locus_tag="VGA15_01845"
     CDS             complement(4852..5367)
                     /locus_tag="VGA15_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9586475.1"
                     /translation="MTVSKTAAVDLAKASRRKDPADGAAESPALQMGELSELLGYSLK
                     RAQLKVFEDFLRCVAPLQLTPAQFSVLLLLDKNPGRNQTEIANTLGILRPNFVAMLDG
                     LESRELCARMRSTNDRRSHILVLTDKGRAVLARAKKLVATKHEARLNELLGAANRAAL
                     LEMLATIAREF"
BASE COUNT         1145 a         1700 c         1702 g         1037 t
ORIGIN      
        1 tggaacgatc ccaaggactt tccatggacc atgggatggc agccgaacta ccagagcgag
       61 agccagatct atgcgaagta tttgctgaaa cagaagcccg acgccaagat cgcggtgctg
      121 tatcagaacg acgactacgg caaggactat ctgaagggcc tgaaggacgg gcttggcgcc
      181 aaggccgcca cgatgatcat tgccgaagaa agcttcgaga ccacggagcc caccatcgac
      241 aaccacatcg tcaagctgaa atcgaccggc gccgatgtgt tcgtcaatat cacgaccccg
      301 aaattcgcgg cgcaggcgat caagaaggtc gcggaaatcg aatggaagcc gctgcacatc
      361 ctcaacaacg ttgcggcgtc ggtcggcagc gtcatcaagc cggcgggcta cgagaatgcg
      421 caaggcatta tctccgccgc ctatctgaag gatgcttccg atccgcagtg ggacaacgat
      481 cccggcatga aggaattcct ggccttcctg gcgaaggatt ttcccgaagg caacaagctc
      541 gacctctcgg tcgtggtcgg ttacggcgtc tcgcagaccc tggtgaaggt tcttcagatg
      601 tgcggcgaca atctcactcg cgaaaacatc atgaagcagg cggctaacct gaaggatttc
      661 cggaccgagg tcctgctgcc cggcatcaag atcaataccg gcccggatga tttcgcgccg
      721 atcagccagc ttcagctgat gaaattcaac ggcgagaagt gggacctgtt cggcgacgtc
      781 atcagcgccg atgtcggcgg ctgacgcagg agtgagcgtg ccccggatgc agcgcagcgc
      841 tcttgcggtg cgctgctgat cccgggtcca tgtgccgctg cgtttgtggt cccggctctg
      901 cggagctgcg caagagcgct gcatcgtgtc cgggacacga taccttcctc acatttacct
      961 tcctcacatt cacctccccc cgcatctccg cgggggtttt cttttgccga aaacggatgg
     1021 ataacggcgt tgcatgtttc catctcaaat cccgtgaaac catgacggac agacgccctc
     1081 tcctgcgcac gatcttcgac gccgccgtgg ccgccgcgca tcccgatgtc gtgctggcag
     1141 cgcatctgcg gccggtgccg aaaggccggg tgatctgcct ggccgccggc aaggccgcag
     1201 ccgccatggc cgcggcagcg gagcggtatt atctcgacga acttggcctg gagccggcac
     1261 ggctattggg catcgcgacc acccgccacg gccatggcgt gccgacgcgg cggatcaggg
     1321 tggtcgaagc cggccatccg gtacccgacg aagccggaat gaagggcgcg gaggacagtt
     1381 tgcggctggc gcagagcgcc ggcgccgacg atctcttgct ggtactgctg tcgggcggcg
     1441 gttcggccaa ctggaccgcg cccgccgaag gcgtgtcgtt cgcgcagaaa cggcaactga
     1501 cccgcgcgct gttgcgctcg ggcgcgccga tcggcgaggt caacaccgtt cgcaagcacc
     1561 tgtcgcggat caagggcggc cggctcgcgc gcgccgggaa gctggccgag atcgtgacgc
     1621 tggcgatatc ggacgtgccg cacgacgatc cgtccgccat cgcctcggga ccgacagtgc
     1681 cggattcgac cacgcttgcg gatgcgcgcg cgcttgtcgc gaaatatggc ttcaaggtcg
     1741 acgaagcgat ccgtgacgcg ctcaatgacg caaagaacga gagctgcaag cctggcgacg
     1801 ctgcattcgc gcgcgcgcaa tttgagttga ttgcgcggcc aaaagcttcg atcgacgccg
     1861 cgatcaggct ggcgcgcgac gccggctacg agatcatcga tctcggcgcc gatctcgaag
     1921 gcgaggtccg caacgtcgcg gccgatcacg cccaacttgc gttgcgcgcg cgcgcgcaag
     1981 gcaagcgtgt ggcgatcgtg tcgggcggcg aactcaccgt cacggtgcgc ggcaacggcc
     2041 gcggcggccc caaccaggaa tacgcactgg cgctggccgg gcttctcaag gacacatccg
     2101 gcattgcggc actggctggc gataccgacg gcgccgacgg cggcgccggc aaggcgaccg
     2161 atcccgccgg cgcgatgatc gaccaaaata ccttcgccaa gatgacatcg ctcggtctcg
     2221 acccaggcgc ctatctcgac aacaacgatg ccaccgcatt tttcgccgcg accggcgatc
     2281 tcctgctgac cggcccgacg ctgaccaatg tgaacgatat cagggtcatt ctggtggatg
     2341 gcacgtaggc cttcaagaac ggcagcggca gtccgggacg cgacgcatcc acagatgcgc
     2401 gattgcacat cggaaacggc agggttgcgc ccggacatcc ctgcgaggtc gtgctcaccc
     2461 tcgccggcgc gaaaaacatt aacgccttgt tgagcgaatt ctggggaact tgcgggaact
     2521 accaacatct caaggatgca tttcacctgc ctcggcctct aataaagctc gtttaaggag
     2581 agctgcgaaa ttgcggcagg gggacgcgaa atgacgggtt ctatggagct tgccatcgat
     2641 ccgaagccgg cgcaccggac ttggctgctc gaggaggtta tcaacaatct gtcgctcgga
     2701 ctcattgtct tcgacaacaa gcgtgaggtc gtgttctgca acaagcggta catggaaatc
     2761 tacgggcttt cgtccgagca ggtaaagccg gggactccaa ccagcgaact tatccagcac
     2821 cggctgaatt tgggacttaa ggtcccctcg aagcccgacg actacatccg ggagcgcgtt
     2881 gcaaacgccg tcgttccagg cgcgacggtc catgaattca ccgatggccg gatcattgcc
     2941 tataccgtct atccgatgcc ggacggcggc ggaatgacca ctcatgagga catcaccggc
     3001 cgggaggaat tgagcgctcg tctgaaaatg ctgtacgagc ttggtagaca gcaggaagag
     3061 aatctgcgga tacgaaacct gcaattcgac acggcgatca acaacatgtc gcagggacta
     3121 tgcttcttcg acgcagccca acggctgatc gtgtgcaacg accgctacgt cgagatgtat
     3181 ggtcttccgc ccgaccgcgt tggtcccggc accccgctgg ccgacattgt cgacatgcgg
     3241 tttgaagcag gcagctttcc cgcgatgtcc agggatgaat atctgcattg gcgcagcaaa
     3301 attgcggtat ctgccaaacc caccgagagc atcgtcgaac tgcgcaacgg gcgcaccttc
     3361 aagatccgtc atcggccgat gcccgacctt ggctgggtcg ccacccatga agacatcacc
     3421 gagcagcggc gatccgaggt caagatcgaa tatatggctc accacgactc gctcaccggt
     3481 cttgccaacc gggtgttgtt gaacgagcgg ctcgaagacg cgatcggccg gatcaatcgc
     3541 gaggagatgg tggcggtcca tcaccttgat ctcgatcagt tcaaggccgt gaacgatacg
     3601 ttcggtcatc cggtcggtga caagctcctg aaaatagtcg cagacaggtt gcgcggcctg
     3661 gttcgcgaga ccgacacgat cgcacggatg ggcggcgatg aattcgtcat cgtgcagccc
     3721 ttcatcgcag atcccgccga ggcaaccgct cttgcgcacc gcgtcatcaa gctgatgagc
     3781 gagccctacg gcatcgacgg ccaccaggcc gtcatcggcg ccagcatcgg cattgcggtc
     3841 ggtcctggcg acggatcgag ggctgacaag ctgctgcgca atgccgacct cgcgctatac
     3901 cgggcgaaag gcgatggacg tggcacgttc cggtttttcg agcccgcgat ggacctgcaa
     3961 atgctaaccc gtcgcatcat ggaacaggac ctgcgcaaag cactgcccgc cggcgagttc
     4021 gaactgtact accagccggt ggtaaatctg gcgaacagcg taatcagcgg attcgaggcc
     4081 ctgatccgct ggaatcatcc ggaacgtggc atagtttctc ctgccgcatt cattccgttg
     4141 gcggaagaga tcggctttat cgtcccgttg ggagagtggg taatccggca ggcatgtgcc
     4201 accgcagccc gatggccaga agatctccgt gtcgccgtca atatttcggc ggttcaattc
     4261 cgtaatcccg gcctgatgca ggtcatcgtt ggggcactcg ctgcctcggg cctggacccg
     4321 acacggctgg agatcgaaat taccgagacc gtcctgctgc acaacaggga agcgacactt
     4381 gccgtgcttc accagttgcg agcactcggt atccggatcg ccatggacga ttttggcacc
     4441 ggatattcct cgctcaccta tctccagtgt tttccgttcg acaagatcaa gattgaccgt
     4501 tcgttcgtaa aggacattac ggaaaacgca ggctcgctca atatcgtgcg ggcggtcgcg
     4561 gcgctcgcaa acggaatggg catgacggcc acggccgaag gcgtcgaaac cagagaacag
     4621 ctcgaccaga tcacgtccga gggttgtacc gaaatgcagg gattcctgtt cagtcagccg
     4681 atccgcgcac atgagatcga acggctgttc ctttcagagc acgaggcgcg gaaaggtctt
     4741 ggcggtactg tcgcagcttg atcgcactca tcaatgtcaa cgatatcagc gtgatcctgg
     4801 tggatggggc gtagatcccc tcagcgtcat tgcgaggacc gaggccgcgc ctcaaaactc
     4861 tcttgcgatc gtcgccagca tttcgagcag tgcggcgcgg ttggcggcgc ccagcaactc
     4921 gttgagacgc gcctcgtgct tggtagcgac gagctttttg gcgcgcgcca gcaccgcacg
     4981 gcccttgtcg gtcaggacaa gaatgtggga gcggcgatcg ttggtcgaac gcatccgcgc
     5041 gcacagctcg cggctctcca ggccgtcaag catcgccacg aaattcggcc ggaggatgcc
     5101 gagcgtgttg gcgatctcgg tctggttacg tcccggattc ttgtcgagca gcagcagcac
     5161 tgaaaattgc gccggggtaa gctgcagcgg cgcgacgcag cgcagaaaat cctcgaagac
     5221 cttcagttgc gcgcgcttga gcgaatagcc cagcaactcg gacaattcgc ccatctgcag
     5281 cgccgggctt tccgcggcac cgtcggcagg gtctttgcgg cgagacgcct tggcgagatc
     5341 cacggcggcg gttttggaaa cagtcatcgc tcgaagctcg caatcgcgct agagtccgat
     5401 ccggcttcgc tgagtcggac tcgtcgctta tccttttgtt tatccatgat cttttccgaa
     5461 aaccggcctc atttttcggg atcgtgccgc gggcttgcaa cggcccggcg ggcgttacgg
     5521 gcttgagatt atatttgata attgttatcc accataccaa atatcgacag cttctttcaa
     5581 tggc
//
LOCUS       DASWGM010000055         3824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2361446,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000055 DASWGM010000000
VERSION     DASWGM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361446"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..693
                     /locus_tag="VGA15_01850"
     CDS             241..693
                     /locus_tag="VGA15_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9586476.1"
                     /translation="MLGLTINGSAYNVDVEPDTPLLWAIREQVGLTGTKYGCGIAQCG
                     ACTVHIDGEAVRSCVVTVGSLRPEQKIVTIEGLERNGALHAVQKAWLEHDVPQCGYCQ
                     CGMIMAAAALLKEKPKPTDADIDAAITNICRCGTFQQARAAIHAAANA"
     gene            705..2879
                     /locus_tag="VGA15_01855"
     CDS             705..2879
                     /locus_tag="VGA15_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9586477.1"
                     /translation="MNLGTKINRRAFVAGTFAVGGGLAIGLDIPLGAGAVRAADGSPE
                     VNAWVVIRPDDTVVIRIARSEMGQGTLTGLAQLVAEELECDWSKVTTEYPTPGQNLAR
                     NRVWKDFGTGGSRGIRDSHEYVRQGGATAREVLIQAAAQEWRVPASECKAANSIITHT
                     PSGRSSTYGKVAEAAAKLQPPVDVKLKDPKDWKLAGKGVKRLDTPDKLTGKQVYGIDV
                     KLPGMVNAAIKDCPVFGGKLKSFDAAKVASMPGVKKVVQVGDSAVAVVADTWWHAKTA
                     LDALPIDWDEGENAKVSSEYLAGWLKEGLDAEQAFVGNQNGDVRTALAGAAKKVEAVY
                     TYPFQNHAPMEPMNATVRYTPDSCEVWTPTQNGEAALAATAEAAELPVAKCDVHKMLV
                     GGGFGRRVARQDWVTKAVLIAKQIPGTPLKLIWSREEDMLHGQFHPLTQAKLVGGFDA
                     QGNLSALHMRISGQSILSSQRPSALQNGKDPLVFQGLDASGQFSIGYTIPNLLIDHAM
                     RNPHVPPGFWRGVNVNHNAIYLECFMDELAHATGQDPLDFRRKLMAEHPKHLAVLNAV
                     AAKAGWGKPAVQGVHRGLAQFMGFNSYTAAVAEISMNNDKLKVHRIVAATDCGTAVNP
                     AQIERQIAGSFVYGLSALFYQECTVKNGRIEQENFDSYPSMRIAAMPHVESIVMPSGG
                     FWGGVGEPTIAVAAPAVLNAIFAATGKRIRSFPLSKHGIVTV"
     gene            complement(3028..3339)
                     /locus_tag="VGA15_01860"
     CDS             complement(3028..3339)
                     /locus_tag="VGA15_01860"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HEX9586478.1"
                     /translation="MRFPKGKSGNPGGRPKVLGEVQELARQYAPTAIVELARLALKAK
                     SETARIAAIRELLDRGYGRSRQAMEITAPAGDPLQLLFEELDALSRKTDRYKQEPQRP
                     A"
BASE COUNT          761 a         1172 c         1223 g          668 t
ORIGIN      
        1 ggctcctgac gatcggcacg atcatcgtcg agattcccgt cgcgttcgcc gcctttacta
       61 tcttgagcca ctaagcgctc gcaggaccgc tcaggccgcc tcgccgacga atacgacgcg
      121 gcgcaggaac gtggtgaggt cccaaagggt aggcctaaaa gtattcccgg cgagaatact
      181 ttgcagccga cagccgatga cattgcgccg gcaaaacgac accaaaaagg gaggaagtcg
      241 atgcttggcc tgaccattaa cggatctgcg tacaatgtgg acgtcgaacc ggatacgccg
      301 cttttgtggg cgatccgaga acaggtcggc ctgaccggaa ccaagtacgg ctgcggcatt
      361 gcgcagtgcg gtgcctgcac ggttcacatc gacggcgagg cggtgcgttc ttgcgttgtg
      421 accgtcggtt cgctccgacc cgagcagaag atcgtcacta tcgaagggct cgagcggaac
      481 ggcgcgctgc acgcggtgca gaaggcttgg ctcgagcacg acgttccgca atgcggttac
      541 tgccagtgtg ggatgatcat ggcagccgcg gcgctgctga aggagaagcc gaagccgacc
      601 gacgccgata tcgatgcggc gattaccaac atctgccgtt gcgggacgtt ccagcaggca
      661 cgcgcggcga tccacgccgc cgccaacgcg tgagggaggc ggtcatgaat ctcggcacca
      721 aaatcaatcg tcgagccttc gtagccggca cctttgcagt cggtggtggg ctcgccatcg
      781 gcctcgacat cccgcttggc gcgggcgccg tgcgtgccgc ggatggctcg cccgaggtca
      841 atgcctgggt ggtgatccgc cccgacgaca cagtggtgat ccgcatcgcg cgttcggaga
      901 tgggccaggg cacgctcacc ggcctcgccc agctggtcgc cgaggaactg gaatgcgact
      961 ggtcgaaggt aacgaccgaa tatccgacgc ctgggcagaa cctggcgcgc aatcgcgtgt
     1021 ggaaggattt cggcacgggc ggcagccgcg gcatccgcga ctcgcacgaa tatgtgcgcc
     1081 aaggcggcgc gaccgcgcgc gaggtgctga tccaggcggc ggcgcaggaa tggagggtgc
     1141 ccgcgtccga atgcaaggcg gcaaacagca tcatcacgca cacgccgtcc ggccgcagta
     1201 gtacctatgg caaggtcgcg gaagccgctg caaagctgca gcccccagtg gatgtgaagc
     1261 tgaaggatcc gaaggattgg aagcttgccg ggaaaggcgt gaagcggctc gacacccccg
     1321 acaagctgac cggaaagcag gtctacggga tcgatgttaa gcttccggga atggtcaacg
     1381 cggcgatcaa ggattgtccg gtgttcggcg gcaaattgaa gagcttcgat gccgccaagg
     1441 tcgcaagcat gccgggcgtg aaaaaggtcg tacaggtcgg cgattctgcc gtcgcggtgg
     1501 tggccgacac ctggtggcac gctaagaccg cgctcgacgc gttgcccatc gactgggacg
     1561 aaggcgaaaa cgcgaaggtc tcgtccgaat accttgcggg ctggctaaag gaaggcctcg
     1621 acgccgagca agcttttgtc ggcaaccaga acggcgacgt caggaccgcc ctcgccggcg
     1681 ccgccaagaa ggtggaagcg gtctacacct atccgttcca gaaccacgcg ccaatggagc
     1741 cgatgaacgc cacggtgcgc tacacgcccg acagctgcga ggtttggacg cccacgcaga
     1801 acggtgaagc cgcccttgcg gccaccgccg aggcagctga actaccggtg gcgaaatgcg
     1861 acgtgcacaa gatgcttgtc ggcggcggct tcggccggcg ggtggcgcgc caggactggg
     1921 tgacgaaagc ggtcctgatc gcgaagcaga ttcccggtac gccgctaaag ctgatctggt
     1981 cgcgcgaaga ggatatgctg catggccagt tccacccgct cacgcaggcc aagctggtgg
     2041 gcggattcga cgcgcagggg aacttgagcg ccttgcacat gcgcatctcc gggcagtcga
     2101 tcctgagttc gcaacgccct agtgcgttac agaacggcaa ggacccgctg gtcttccagg
     2161 ggctcgatgc gagcgggcag ttctcaatcg gctacacgat cccgaacctg ctgattgacc
     2221 atgcaatgcg caaccctcat gtgccgcccg gtttctggcg cggcgtcaac gtcaaccaca
     2281 acgccatcta cctcgagtgc ttcatggatg aactggcgca cgcgacaggc caggatccac
     2341 tcgatttccg gcgcaagctg atggcggagc atccgaagca ccttgccgtg ctcaatgccg
     2401 tcgccgcaaa ggcaggctgg ggcaagccag cggtgcaagg cgtgcaccgc ggtctggcgc
     2461 agttcatggg tttcaatagc tatacggccg cggtcgcaga aatctccatg aacaacgaca
     2521 agctgaaagt gcaccgcatc gtggcggcga ccgactgcgg gaccgctgtc aatccagcgc
     2581 agatcgagcg gcagattgcc ggctctttcg tctacggcct ctccgccctc ttctatcagg
     2641 aatgcacggt gaagaacggc cgcattgaac aggagaattt cgacagctat ccgtcgatgc
     2701 gcatcgccgc aatgccgcac gtggaatcga tagtgatgcc gagcggcggt ttctggggcg
     2761 gcgttggcga accgaccatt gcggtggcgg caccggccgt gctcaatgcg atcttcgcag
     2821 ccacgggcaa acgcatccgc tcattcccgc tgtccaagca cggcatcgtg acggtttgag
     2881 gaggcaggag agcggccgtg gcgcagccgc tccacatgcc ggcattgctc gcatggcaga
     2941 ccgaatttcg gcggagcgag gtggcttatg cggcgtcaat ctcgctcgac gacattccgc
     3001 gaaaaacggg ggccgcaagc gttggcttca agcgggtctt tggggctcct gcttataacg
     3061 atcggtcttt ctggacaacg catcgagctc ctcaaatagc agctgaagcg gatcacccgc
     3121 gggcgcagtg atctccatgg cttgtcgaga cctgccataa ccccggtcga ggagctcgcg
     3181 gattgccgcg atccgagcgg tctcgctctt tgctttcaac gccaagcgtg ccagctcgac
     3241 gatggctgta ggcgcatatt ggcgcgccag ttcctgcact tcgccgagaa ccttcgggcg
     3301 accaccgggg ttgccgctct tgcctttagg aaacctcatc tcttgcctct gttagctctc
     3361 tgttctcagg ggctctttga gtttgaggtt tttcatgcag aagccgccac caaggcagaa
     3421 gatcgccctt cgccgaccgt ggctcgcgga ggtcacgatc aatctatgaa acgtcatgcg
     3481 ccggtaccat tagtatgccc gcgggccacg gatcatcggt ctagctggac ccagccctcg
     3541 cctcgtttct tatcgtctct tcgtcgacat gtcgacgtgc gccgccgtca gcatggccgg
     3601 cctcgcgtcc gccagcgtgc gggccagccc tgccacagtg ctgcagcccc tatgccagcc
     3661 cctccggttg gttcaagctg tttcagcagg gttcggatgc gacgtgactc aaatcgcacg
     3721 cagcggggct gccataacga taagaaagtt aacaagaagg atgcgaaggc caaaccggac
     3781 gggaagctca cgtcatcgtc gtagcggcga ggcgctcacc gcca
//
LOCUS       DASWGM010000056         6791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1251120,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000056 DASWGM010000000
VERSION     DASWGM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251120"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VGA15_01865"
     CDS             <1..145
                     /locus_tag="VGA15_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9586479.1"
                     /translation="DAEAFDRAVDLRIMRIRRKIEMDPTKPAVIRTIRGGGYLFSPAG
                     DKA"
     gene            466..1077
                     /locus_tag="VGA15_01870"
     CDS             466..1077
                     /locus_tag="VGA15_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9586480.1"
                     /translation="MQNVVAINAAAREGIIAAQATSDEMLLESIAEGGRTAMHTLYSR
                     HNVRVYRFVLRMVRDTTMAEDLVSQVFLDVWRTASQFEGRSQVSTWLLSIARFKALTA
                     MRQRRHEDIDQEDVLEIADQADTPEASLNRSNTSEILRACVAKLSPAHREIINLVYYH
                     EKSVEEAGAIIGIPQSTVKTRMFYARKQLADLLKGAGVDNLAA"
     gene            1258..1461
                     /locus_tag="VGA15_01875"
     CDS             1258..1461
                     /locus_tag="VGA15_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586481.1"
                     /translation="MLKPTLRFFIVPLGAICALASLSAQNTTPRAQRDTGSMFVHQQV
                     VDCSHKNPAEVCVISDVSETSAR"
     gene            1764..2462
                     /locus_tag="VGA15_01880"
     CDS             1764..2462
                     /locus_tag="VGA15_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX9586482.1"
                     /translation="MFNLVLALLAGVVTIAAPCTLPMLPILLGASVGQAGKARPALIA
                     LGFVISFSAAALLLSAITRVFDFDPNVLRTGAAIMLLGFGVLMIWPAPFERFSALVGG
                     LTNGGPGDSAFRQGNIGGFVLGTTLGLVWTPCAGPVLGSILTIVATSRDTAWASALLV
                     VYAVGAAIPMLAIAYGGQAVTTRVRSIARISPRLQQGFGVVVIAFAVASYFQYDTLIV
                     AWLTGFYPTGQIGL"
     gene            2491..3057
                     /locus_tag="VGA15_01885"
     CDS             2491..3057
                     /locus_tag="VGA15_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9586483.1"
                     /translation="MTIRSLAATAAVVAACLTSTAIPCLSGELERDQTVPFAAADLQN
                     AAPNFAGISNWFNSAPLNMTDLRGKVVLVDFWTYGCVNCVNTLPHVTELYAKYRDRGL
                     VVVGVHTPEFPFERSASNVQAALKRHGITYPVAQDNDSQTWNAYRNQYWPAQYIIDQS
                     GKIVFRHDGEGQYEQMDRTIARLLNASS"
     gene            3140..4549
                     /locus_tag="VGA15_01890"
     CDS             3140..4549
                     /locus_tag="VGA15_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX9586484.1"
                     /translation="MFEAWDAVVLARAQFAFTMSFHIVFPAFSIGLASYLAVLEALWL
                     WTGREVFINLFNYWLKIFAIAFGMGVVSGIVMSYQFGTNWSAFSDRAGPVIGPLMAYE
                     VLTAFFLEAGFLGVMLFGLERVGHRLHFFATLMVATGTLISAFWILSANSWMQTPAGY
                     AVNADGQFVAADWLKVIFNPSFPYRLVHMVLAAYLTTALVVGAVGAFHLLRDSHQAGP
                     RVMFSMAMWMATLVAPIQILAGDQHGLNTLEHQPTKIMAMEGHFQSHPHGAPLILFGL
                     PDQAAGEVKYAIEVPMLGSLILKHSIDAPLAGLDTVPRENWPPVPITFWSFRIMVGMG
                     FLMVGLGLFSLWSRWRGTLYQSRLLHMFAMAMGPAGFIAVLAGWITTETGRQPFTVYG
                     LLRTVESASPLAAPAVASSLIGFVIVYFIVYAAGLTYLFRLMASAPHHGEQGPSADLP
                     ARAAGITPAAGAATQGSAR"
     gene            4546..5559
                     /gene="cydB"
                     /locus_tag="VGA15_01895"
     CDS             4546..5559
                     /gene="cydB"
                     /locus_tag="VGA15_01895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX9586485.1"
                     /translation="MTALFDIATIWAFIIAFAVFVYVVMDGFDLGLGLLFPLFPNKAD
                     RDVIMNSVAPVWDGNETWLVLGGGGMMAAFPLAYAVLMPALYTPMVAMLIGLIFRGVA
                     FEFRWRTTQSQRNRWDIAFAGGSLLATLAQGIALGAMLQGVHVEGRHYAGGWWDWLTP
                     FSILTGLALVVGYALLGATWLVLKTEGELRDKAYRLSWALLFAMLGAIGAVSIATPFL
                     HIHYTQRWFTWPNIILTAPVPIAVAGVTVLLLRSLANKLDTQPFFLSLALFALSYAGL
                     GISMYPYIVPQSITIWQAASPENSQVFMLFGVAVLVPLILGYTAWAYWVFRGKVRPGS
                     GYH"
     gene            5559..5687
                     /locus_tag="VGA15_01900"
     CDS             5559..5687
                     /locus_tag="VGA15_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 domain-containing protein"
                     /protein_id="HEX9586486.1"
                     /translation="MRQEQNPRPLAQRLLWFAALWLGGVGTVGIVAYGLRLWIAPK"
     gene            5788..6381
                     /locus_tag="VGA15_01905"
     CDS             5788..6381
                     /locus_tag="VGA15_01905"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9586487.1"
                     /translation="MTKFRHLIWMIIAAAGLMLSVSQSFAQKPDVGDQPGLLPDDSVE
                     LDPEYRRTAVLYRTTEAPGTIIISTAERHLYLIQGNGRALRYGIGVGREGFQWQGLLN
                     ISRKAEWPDWTPPPEMIARQPYLPRFMAGGPGNPLGARAMYLGTTVYRIHGTNRPDTI
                     GTAVSSGCFRLVNADVADLYERVPVGTRVVVRQKPEL"
     gene            6439..>6791
                     /locus_tag="VGA15_01910"
     CDS             6439..>6791
                     /locus_tag="VGA15_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9586488.1"
                     /translation="MRTFRGGLLIGLAVAVAVAAIAITYERYDTRTLKRTIRRGEVLC
                     GVNTGLPGFSIPDDKGNWTGFDVDFCRAVAAAIFDDPSKAKFVPLDANERFKELQNRK
                     VDILSRNSTWSMSRET"
BASE COUNT         1176 a         2186 c         2053 g         1376 t
ORIGIN      
        1 cgacgccgaa gccttcgatc gcgccgtgga tctgcggatc atgcggatcc ggcgcaagat
       61 cgagatggac ccgaccaagc ccgcggtgat ccgcaccatc aggggcggcg gctatctgtt
      121 ctcgcccgcc ggcgacaagg cctgatccga atcccgagcg agccgtgaat tgaccccctc
      181 tttccgcagg ccgggagaga tgaaaagagg ccaatccttc cgcggtgagc ccaatttcgt
      241 tcacgcgggg atgaggaact ttttcgcggc tgcaatcttc cagatttgcg atcattgaaa
      301 ttgctggaac tcctcccccg aatgtttcgt cgcggccgca gcgacgaaac aattctcctg
      361 cgcatgaaac cattttcccc tttgcgtgca gacgtcccga aacgttcgct gactatcaat
      421 ctgcccaacg aggcgccgcc aagcggcgaa tattgggagc ctgtcatgca gaacgtcgtc
      481 gccatcaacg ccgcagcccg cgaaggcatc atcgccgccc aggcgacctc ggacgaaatg
      541 ctgctggaga gcattgccga aggcggccgg accgcgatgc ataccctcta ctcgcgccac
      601 aatgtgcggg tttaccgttt cgtgcttcgc atggtgcgcg acaccaccat ggcggaagac
      661 ctcgtcagtc aggtttttct cgatgtctgg cgcaccgcca gccagttcga gggtcgttcc
      721 caggtttcca cctggctgtt gtcgatcgcc cgcttcaagg cgctgaccgc gatgcgtcag
      781 cgccggcacg aggacatcga tcaggaggac gtgctcgaaa tcgccgatca ggccgatacg
      841 cccgaagcct cgctgaaccg cagcaatacc agcgagattc tgcgcgcctg cgtggcaaag
      901 ctgtcgccgg cgcatcgcga gatcatcaac ctggtttact accacgagaa atcggtggaa
      961 gaggccggcg cgatcatcgg gattccccag agcacggtga agacgcggat gttctatgcc
     1021 cgcaagcaac tcgccgacct gctcaaggga gccggtgtcg ataacctggc tgcgtgaaaa
     1081 taacaactag atcaatgctt tactggctat ctgacttgcg aaggagcgga cccggttttc
     1141 gcaatagtta ccggccacga aacaattgaa acaattcatg acatcagccg gaaaaacttc
     1201 gctgctacac cgacaagcac caacgccggg cacgagaagc gcagagaagt gcgaaccatg
     1261 ttgaaaccga ccctccgctt cttcatcgtc cctctcggtg cgatctgcgc cctggcctcg
     1321 ctgtccgccc agaacacgac gccccgcgcc caacgcgata ccggttcgat gttcgtgcac
     1381 cagcaggtgg tcgattgcag ccacaagaat cctgccgagg tctgcgtgat ctccgacgtc
     1441 agcgagacta gcgcccgctg acgcccaagt tttctgcccc caagaacctc caacgacccg
     1501 gacgggatgc ccccctccgg gtcgctctgc ttttggggac agccagagcc gttctccgtc
     1561 ctgttcttat tgccatttac tgcttctgat gacgcaggcg attcatcgat gttgggattg
     1621 aactcgttcg cgcttcacat cgaaagcctc acgaacacgt gacgcggcaa aacgtaacgc
     1681 ctcgcctcac cgcatcgaag gaccctacga gctctgacgt ggccgcaagg cgcagatgag
     1741 agctctctga tgggtcctgt ctgatgttca acctcgttct tgccctgctc gccggcgttg
     1801 tgaccatcgc cgcaccctgc acgctcccga tgctgccgat ccttctgggg gcgtcggtcg
     1861 gccaagccgg caaggcgcga ccggcgctga tcgcgctcgg ttttgtgatc tcgttttcgg
     1921 ccgccgcatt gttgctcagc gccatcaccc gcgtcttcga tttcgatccg aatgttctgc
     1981 ggacaggcgc ggcgatcatg ctgctcggct ttggagtttt gatgatctgg ccggcaccgt
     2041 tcgaacggtt ttcggccctg gtcggcgggc tcaccaacgg cggccccggc gacagcgcgt
     2101 tccggcaagg caacattggc ggcttcgtgc tcggcaccac gctcggtctg gtgtggacgc
     2161 cctgcgccgg cccggtgctc ggctcgatcc tcaccatcgt cgcgacatcc agggataccg
     2221 catgggcgag cgcgctgctg gtggtctacg ccgtgggcgc ggcgatcccg atgctggcga
     2281 tcgcctatgg cggccaggcc gtcaccaccc gcgtccgcag tatcgcgcgg atctccccca
     2341 ggctgcagca gggatttggc gtcgtcgtca tcgccttcgc ggtcgcctcc tactttcaat
     2401 acgacacgct gatcgtggcg tggctgacag gattttaccc gaccggccag atcggcctgt
     2461 aatcaaccgc gcccgataat ggagtttgcc atgacgatac gttccctcgc cgccaccgct
     2521 gccgtggttg ccgcttgcct gacgagcact gccataccct gcctcagcgg cgagcttgaa
     2581 cgcgaccaga cggttccgtt cgcggccgcc gacctccaga acgccgcacc caacttcgcc
     2641 ggcatcagca actggttcaa ttccgcgccg ctcaacatga cggacctgcg cggcaaggtg
     2701 gtgctggtgg atttctggac ctatggctgt gtcaactgcg tcaatacgct gccgcacgtc
     2761 accgagctct atgcgaaata cagggatcgc ggcctcgtcg tcgtcggcgt ccacacgccg
     2821 gaattcccgt tcgagcgatc ggcatccaac gtgcaggccg cgctgaaacg gcacggcatc
     2881 acctatccgg tcgcgcagga caacgattcc cagacctgga acgcctatcg caaccagtat
     2941 tggccggcgc aatatatcat cgaccagagc ggaaaaatcg tgttccggca cgacggcgaa
     3001 ggccagtacg aacagatgga tcgcaccatc gcgcgcctgc tcaacgcctc aagctgagcg
     3061 gtcctgcgca cggcgctgta ccatcgtcac ggtcggcatg gtaaggtccg cgtcggaata
     3121 gcgacgacgg atgactgtca tgttcgaggc ctgggatgcc gtggtattgg cgcgggcgca
     3181 gttcgcgttc acgatgtcgt ttcacatcgt gtttccggcg ttctcgatcg gacttgccag
     3241 ctatctcgcg gtgctcgaag cgctctggct gtggaccgga cgcgaggtct tcatcaatct
     3301 gttcaattat tggctgaaga tcttcgccat cgcgttcggg atgggcgtgg tgtcgggcat
     3361 cgtgatgtcc taccagttcg gcaccaactg gtcggcgttt tcggacagag cgggccccgt
     3421 gatcggcccg ctgatggcct acgaggtgct caccgcgttc ttcctcgaag cgggttttct
     3481 cggcgtcatg ctgttcgggc tggagcgggt cggccaccgg ctgcacttct tcgcgacctt
     3541 gatggtcgcg accggcacgc tgatttccgc gttctggatc ctctccgcca attcctggat
     3601 gcagacgccg gccggctacg ccgtcaacgc cgacggtcag ttcgtcgccg ccgattggct
     3661 caaggtcatc ttcaacccat cgtttcccta ccgcctggtg catatggtgc tggcggccta
     3721 tctcaccacg gcgctggtgg tcggcgcggt cggcgcgttt cacctgttgc gcgattccca
     3781 tcaggccggt ccgcgggtga tgttttcgat ggcgatgtgg atggccacat tggtggcgcc
     3841 gatccagatc ctggccggcg accagcacgg cctcaacaca ctggagcatc aacccaccaa
     3901 gatcatggca atggagggcc atttccagag tcacccgcac ggcgcaccgc tgatcctgtt
     3961 cggcctgccg gatcaggccg ccggcgaggt caaatacgcg attgaagttc cgatgctggg
     4021 atcgctgatc ctcaaacatt caattgatgc gccgcttgcc ggcctcgaca ccgtgccgcg
     4081 cgaaaactgg ccgccggtgc ctatcacatt ctggtcgttc cggatcatgg tcggaatggg
     4141 ctttctgatg gttgggctcg gcctgttcag cctgtggagc cgttggcgcg gcacgctcta
     4201 ccaatcccgg ttgctgcaca tgttcgcgat ggcgatgggt ccggcgggtt tcatcgccgt
     4261 gctcgccggc tggatcacca ccgagaccgg gcgccaaccg ttcaccgtgt acggcctgtt
     4321 gaggacggtc gaatccgcct cgccgcttgc cgcgcccgcg gtggcctcgt cgctgatcgg
     4381 cttcgtcatc gtctatttta tcgtgtacgc ggcgggcctg acttacctgt tccggttgat
     4441 ggcatctgcg ccgcatcacg gcgaacaggg accgtccgcc gatctaccgg cccgtgccgc
     4501 cggcatcacg ccggcagcgg gtgcagcaac gcaggggtca gcacgatgac cgccttgttc
     4561 gacatcgcaa ccatctgggc gttcatcatc gcgttcgcgg tctttgtcta tgtggtgatg
     4621 gacggattcg atctcggcct cggcctgctg tttccgctgt ttccgaacaa ggccgaccgc
     4681 gacgtgatca tgaacagcgt cgcgccggtg tgggacggca atgaaacctg gctggtgctc
     4741 ggcggcggtg gaatgatggc ggcgtttccg ctggcctacg cggtgctgat gccggcactt
     4801 tacacgccga tggttgcgat gctgatcggc ctgatctttc gcggcgtcgc cttcgaattt
     4861 cgctggcgga cgacgcagag ccagcgcaat cgctgggata tcgcattcgc cggcggatcg
     4921 ctgctggcga cgctggcgca aggcattgcg ctcggcgcca tgctgcaggg cgtgcatgtc
     4981 gaggggcgac attatgccgg cggctggtgg gactggctga cgcccttcag catcctgacc
     5041 ggccttgcat tggtggtcgg ctacgccctg ctgggggcga catggctggt gctgaagacc
     5101 gagggcgagc ttcgcgacaa ggcctatcgc ctgagctggg ctctcctgtt cgcgatgtta
     5161 ggggccatcg gcgcggtcag catcgccacc ccgttcctcc acatccacta tacccagcgc
     5221 tggttcacct ggcccaacat catcctgacc gcaccggtac cgatcgcggt agccggcgtt
     5281 accgtcctgc tgttgcgcag cctcgccaac aaactcgaca cccagccgtt ctttctgtcg
     5341 ctggcgctgt tcgcgctgtc ctatgccggc ctcggcatca gcatgtatcc ctatatcgtg
     5401 ccgcagagca tcaccatctg gcaggcggca tcgcccgaga acagccaggt gttcatgctg
     5461 ttcggcgtgg ccgtgctggt gccgctcatt ctcggttata cggcgtgggc ttattgggta
     5521 ttccgcggca aggtaaggcc cgggagcggc tatcattgat gcgtcaggag caaaacccgc
     5581 gaccgctggc gcaacgcctg ttgtggttcg cagcgctctg gctcggcggc gtcggcaccg
     5641 taggcatcgt ggcctatggc ttgcgactgt ggattgcgcc gaaatgatcg gcgatacatt
     5701 accaccgatc gctgcgattt tggtggttcc ggtggttcgc cggggatggt agaccaatat
     5761 cgttgagctg aacgaggaga gtttcgaatg acgaaatttc ggcacctgat ctggatgata
     5821 attgccgcag ccggtctgat gctttcggtc tcgcagagct ttgctcaaaa acccgacgtc
     5881 ggcgaccagc cgggacttct gcccgatgat tccgtggaat tggacccgga atacaggcgc
     5941 actgcagtgc tttatcgcac cacggaggcg cccggcacga tcatcatctc gaccgcggag
     6001 cgccatctct atctgatcca gggtaatggc cgcgcgctgc gctacggcat cggcgtcgga
     6061 cgcgagggat ttcagtggca gggattgctg aacatttcgc gcaaggccga atggccggac
     6121 tggaccccgc cgccggagat gatcgcgcgc cagccctatc tgccccgctt catggccggc
     6181 ggcccgggca atccgctcgg cgcccgcgcg atgtatctcg gcaccacggt ctatcgcatc
     6241 cacggcacca accgaccgga cacgatcggc accgccgtct cgtccggctg cttccggctg
     6301 gtcaatgccg acgtggcgga cctgtatgag cgggtcccgg tcggcacccg ggtcgtggtg
     6361 cggcagaagc cggaattgta accgcggcgc ctgcttacac ggccgaccta tttttccgat
     6421 cattgcgaga gacaaaccat gcgcacattc cgaggcggcc tgctgattgg gctggcggtc
     6481 gcagtcgcgg tcgcggctat tgccatcacc tatgaacgct atgacacgcg gaccctgaaa
     6541 cgaaccatcc ggcgcggcga agtgctgtgc ggcgtcaaca ccggcctgcc cggtttctcg
     6601 atcccggacg acaagggcaa ctggaccggc ttcgacgtgg atttctgccg cgctgtcgca
     6661 gccgccatct ttgacgaccc gagcaaggca aagttcgtcc cgctcgacgc caacgagcgc
     6721 ttcaaggagc tgcagaaccg caaggtcgat atcctctcgc gcaactcgac atggagcatg
     6781 tcgcgcgaga c
//
LOCUS       DASWGM010000057         4837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2221396,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000057 DASWGM010000000
VERSION     DASWGM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221396"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VGA15_01915"
     CDS             complement(<1..676)
                     /locus_tag="VGA15_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9586489.1"
                     /translation="MDFQLTEQQRRIVAAVRDLAQGEFKVNAQKYMDGTFPWENMKKL
                     ADIGVLGMSVPEEYGGLGLPVFDTALVLEEIAKTCYVTAMATLGEAGVQTRVIATYAP
                     NVIRERILPKVVSGDCILAVCMTEPHAGTDVANYRTNAVISGDRVILNGTKTLISRAR
                     EAGMFVIFTRIDGKPGREGIGCILLEPDTPGFEVTGTYHTMGGENLHEIQFNNCELPL
                     ENLVIRD"
     gene            1086..1922
                     /locus_tag="VGA15_01920"
     CDS             1086..1922
                     /locus_tag="VGA15_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9586490.1"
                     /translation="MVGRAIGKKLTEAWGQPVIVENRSGAGGMIGTPTVAAAPPDGYT
                     LLLVSSTFTINPAIKTSMPFDTIKDFTPVAFIARAPLLVTASNKLPVRSAKELLALAR
                     SKPDQITYASSGPGSINQISAELIALSAGAKLMHVPYKGGAPALNDLLGGHVDIYVSS
                     LPQVLQLAQSGQAKALAVTSAKRTALLPDVPTLEESGIVGFDLWSWWGIVGPAGMPAN
                     VVNALNSEIGKMLNSPELSTFLSNEGAEAETMTPQQFGDLMRLETERWIKVAHEANIS
                     ID"
     gene            complement(2109..3524)
                     /locus_tag="VGA15_01925"
     CDS             complement(2109..3524)
                     /locus_tag="VGA15_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9586491.1"
                     /translation="MQRAQAREQRISQRQQVTQQREQFAQQRQRDTLSRQSAERQIRI
                     DRLQRRVQQFQAQKPQDRQAQRALQAQQGLLQREQLAQKRDLAQRQKLGVAPAAAQRT
                     AVAAAVQAAARGRFAARFRNNADPRAQVALAARLNGWAPRNAWRHHVHAAFVPWLGPL
                     FWPYVYSDIFDYTFWPEAYDDAYWAYAYDDLIDTVFWDNGSPYSAYASIAPDGSVAPD
                     GYVEPGGAMVGSSRLRERSGLSPQALRQLCRNPDRGITAWPFAEITSALRPTSEQRAL
                     LDQLKSAAALAADALKNSCSDTYALTPPGRLRAMINRISATLEAVRMVRPALEAFYNA
                     LSDEQRARFNTLSPRLPAASEDQPQQEAKAESCGASKSSLTGLPIERIDAVLRPTGAQ
                     RGALDRLSAATKKAVQGLQAACPDEVPVTPVGRLQAMEKRLSAMLQAANQMQPALDDF
                     YASLTTEQKARFNTLQQVAGQ"
BASE COUNT          908 a         1488 c         1497 g          944 t
ORIGIN      
        1 cgtcgcgaat cacaagattc tccagcggca attcgcaatt gttgaactgg atctcgtgga
       61 ggttctcgcc gcccatcgtg tggtaggtcc cggtcacctc gaaacccggc gtgtcgggct
      121 ccagcagaat gcagccgata ccctcgcggc caggcttgcc gtcgatccgt gtgaagatga
      181 cgaacatgcc ggcctccctg gctcggctga tcagcgtctt tgtcccattg agaatgacgc
      241 ggtcaccgct aatgacggcg ttggtccggt agttcgctac gtcggtgccg gcgtgcggct
      301 cggtcataca gactgccaat atgcaatcgc cagagacgac cttcggaaga attcgctcgc
      361 gaataacgtt gggagcgtag gtcgcgatca cacgggtctg aacaccggcc tcaccgaggg
      421 tggccatcgc ggtaacataa caagtcttgg caatctcctc gagcaccagc gccgtatcga
      481 ataccggcaa gccaaggccg ccatattcct cgggaaccga catgccaagc acgccaatat
      541 cggcgagctt cttcatgttt tcccaaggaa acgtaccatc catatacttt tgcgcgttta
      601 ccttgaactc cccctgagcg aggtcgcgca ccgcggctac tatccgccgc tgctgttcgg
      661 tgagttggaa gtccattgct tatccttgaa gtgaaaaagg cgacgacccg atcggggcag
      721 aatacatccg ttcagaagcg aactggccga cccgcaccac gagctaaccc cgtttcactg
      781 acggctaaat ccgcaaaagt aatactactg gtgctcttgc catgagggat agacgcctgc
      841 gcatctgcat tttcccgcgt tatggaaccg gcggaaatgt ctctcgcgga tatgatgatt
      901 ataagtcatt ttcgagtgca cgtttgccat aacatctttg gcgcagggag aaacgtatca
      961 tgatcaagac tggagctttc cttgccgcag cacttgtact cacaagctcg gcggtgcgcg
     1021 cgcaaacata tccgtccagg cagatcacgc tgattattcc gttcgcggtc gggggttcga
     1081 acgacatggt cggccgcgct atcggcaaga agcttaccga ggcgtggggt cagccggtga
     1141 tcgtcgaaaa ccgttccggc gcgggcggta tgatcggcac ccctaccgtc gccgccgcgc
     1201 cgccagacgg ttacacgctg ctcctggtgt cgtcgacctt cacgatcaac ccggcgataa
     1261 agacgagcat gccgttcgac acgatcaagg acttcacgcc agttgccttt attgctcgcg
     1321 cgccgctcct ggtcaccgcg tcgaacaagc tcccggtgag atccgccaaa gaattgctcg
     1381 ctctagccag gagcaaaccg gatcagatca cctacgcctc atccggtccc ggcagcatca
     1441 atcagatttc ggccgagctc atcgcgcttt cggcgggagc taagctcatg cacgttccct
     1501 acaagggtgg tgcgccggcg ctcaacgatc tcctcggcgg ccatgtcgac atctatgtat
     1561 cgagtctgcc gcaggtgctg cagctcgccc aaagtggcca ggccaaggcc cttgcggtga
     1621 caagcgccaa gcgaacggcg cttctgccgg acgtaccgac tctcgaggag tcggggatcg
     1681 tcggctttga tctgtggtcc tggtggggca ttgtcggccc ggccgggatg cctgcgaatg
     1741 tcgtcaatgc gctcaactcg gagattggca agatgctgaa ctcacccgaa ctcagcacgt
     1801 ttctctcaaa cgagggcgcc gaagccgaaa ccatgactcc ccagcaattc ggagacttga
     1861 tgcgtttgga aacggagcgc tggatcaagg tcgcgcacga ggcaaatatt tcgatcgatt
     1921 agcgccaagg cggtgaacgc atttgtcgcg atgaggcgat gttagttagc ttcaacattt
     1981 tcttggcttg tcggagactg gatagtcttc cacgcagtcc tgatcggtca cgagacgccg
     2041 cttacgtttg aacgtcggtt ttgcgccaac aacagatacc gccgttcaac aatatggcgg
     2101 cgtcccgctc actgtcccgc gacctgctgc agtgtgttga agcgtgcctt ctgctcggtg
     2161 gtcaggctgg cgtagaagtc gtccaacgcc ggctgcatct ggttggccgc ctgcagcatg
     2221 gccgaaagcc gcttctccat cgcctgcagt cgtccgaccg gcgtaaccgg cacctcgtcg
     2281 gggcacgccg cctgcaaccc ctgtaccgcc ttcttcgtcg ctgcgctcag gcgatcgagc
     2341 gcacccctct gcgcgccagt cgggcgcagc acggcgtcga ttcgttcgat cggcaggccc
     2401 gtcaggctcg acttcgacgc gccgcagctc tcggccttgg cctcctgctg cggctgatct
     2461 tccgatgccg caggaagacg cgggctcaac gtattgaagc gggcccgctg ctcgtcgctc
     2521 agtgcgttgt agaacgcctc cagcgccggc cgcaccatcc gcaccgcctc gagggtggcg
     2581 ctgatacgat tgatcatcgc ccgcagacga ccgggtggcg taagcgcata ggtgtcgctg
     2641 caggagttct tgagcgcgtc ggccgcaagc gccgccgcac tcttcaactg atcgagcaat
     2701 gcgcgttgct ccgatgtcgg ccgcagcgcg cttgtgatct cggcgaacgg ccacgcggtg
     2761 atgcctctgt ccggattgcg gcacaattgc cggagcgcct gcgggcttag gcctgagcgc
     2821 tcgcgcaatc gcgacgaccc gaccatcgcg ccgccaggct cgacgtagcc gtccggcgcg
     2881 acactgccgt cgggcgcgat actggcgtaa gctgaatacg gactgccgtt atcccagaac
     2941 accgtgtcga tcaaatcgtc gtaggcgtag gcccaatagg cgtcgtcata ggcttcgggc
     3001 cagaacgtgt agtcgaagat atcggaatag acgtatggcc agaacagcgg gccgagccaa
     3061 ggcacgaacg cggcgtgcac atggtggcgc caggcattgc gcggtgccca gccgttcaat
     3121 cgcgcggcga gcgcgacttg agcgcgcggg tcggcgttgt tccggaagcg cgcggcaaat
     3181 cgtccgcgcg cggcggcctg cacggcggct gcaaccgcgg tccgttgcgc cgcggccggc
     3241 gccacgccca gcttctgccg ctgcgcgaga tcgcgtttct gcgctagttg ctcgcgctgg
     3301 agtaggcctt gttgagcctg tagcgcgcgt tgcgcctgcc ggtcctgcgg tttttgtgcc
     3361 tgaaattgct gcacgcgccg ctgcaggcga tcgatccgga tctggcgctc ggcactctgg
     3421 cgcgacagcg tatcgcgctg cctctgctgc gcgaactgct cgcgttgctg cgtgacttgc
     3481 tggcgctgcg agatccgctg ttcgcgcgct tgcgcgcgct gcacgatccg ttcctgccgc
     3541 ggcgaacgcg cgctcacggc gggcggtgcg cttgggcgcc gcgattcggc gatgcgcgga
     3601 atcgggggcg aggcgatggc aggaggccgt gacaccgtag ttggacgctg gaagctcggt
     3661 cgcggcgccg cgaagcgggg tgccgccggt cgcgagggtg cggcgaaatg cggctgcggc
     3721 atctgacgcg gcaccatggc cggtcgctgc ggcgcgggag cgggatggaa cgctggcgcg
     3781 gcaggccgag cgatcgcagg tgcagcgggt cgagccaccg ccgcaggcgc ggcgcctcgt
     3841 ctcggctgct gcgcggaggc gccgatgctt cccgcaatca gaaagaggcc aacaaaagaa
     3901 gccgcaaagg gtagcgcaaa gccaactcta tcgggaagca tggtcgtaag ctccctatca
     3961 tacccggccc cgcatgcatc aataacgcgc gagcgcggga aacgatccgc gcatgaacgc
     4021 cgagccagga tcattgctgc gttgcaagac aggctcatta ggacaccgcg cctatttgct
     4081 gcggtgccta agtccggttg tgacagattg tgttgaaaaa gtccttttta gctgatgacc
     4141 gaaaattccc agaaccgctc gtgcgtctcg cgcgccgcga tgtgagggac cacatcgatt
     4201 aacgcaaaag cgaccgctaa cgctcgtatc ggttctgcag aaccttgcag cggctgagat
     4261 cgccaacatt cgacattcgc gaaaagaagg ccttgttcga caacagggca cgccaggtgg
     4321 tcggagcagc ccggcacatg aggagcgaca cgtcgcaaac tgcatcaaag gtgatccggt
     4381 tggagctggc gttgttgcaa ccctcatact tcaggcgtct agctccacat aatttgcggg
     4441 ctgccaatct ggctcattcg ccgaccaaat tcctggacag cggtcttggg caaacgagaa
     4501 cggcacaggt cggctgattt gcccgtcggg caatttgtcg actcgggtgt ccagccccat
     4561 ttgcaaaaat atttctgttt tcgtaccccc caaatcacat ctagaacttt tcgcatcccg
     4621 tcccacacag aggggcgttt cgcgatcgtc acggacgtag ggcacgggat gcggtggacg
     4681 cggcagcgtt ttgcgtgcga cgggatcgca gggcgggttc ttagggactt gtgagcgatc
     4741 aacagcacgc ggacgagaga tgttgttgcg tacggcgaag tcgtgtggtc ctgacgcccc
     4801 gacgctggcg tcaagttttg cggaggtcaa gtcggcc
//
LOCUS       DASWGM010000058         3189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2221471,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000058 DASWGM010000000
VERSION     DASWGM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221471"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VGA15_01930"
     CDS             <1..1093
                     /locus_tag="VGA15_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX9586492.1"
                     /translation="ASFFGVLIFGRSRVPPWFYLFSTAMVALGTTFSAFWIMVNNSWM
                     QVPSGYVMENGAFVPNDWTKILFNSVVWSRFPHMLLASYLTGAFCVAATGAWYMLRKE
                     YHAEARIMLRMGLGLAAVLMPIQLLFGHLVGGYVNTYQPSKMAAIEGRWNDEKPGSEV
                     LFAWPDVEHRRNLFAITLPPPFGSLIDSDSLTAGETGLNSIPPENWPPVVIPFFSFRI
                     MVGCGMVMLGLAWVGSYLSYRHRLAYNRPLLWLTFLSFPLPFIAIITGWFTAEVGRQP
                     WVVFGVLRTADAMTPFLTTREATISLVVFCAVYAFIFLFGTFYIYRLIRTGPARKPFE
                     VPHLAVPNRPMSLADEELIQEPSHAGAGE"
     gene            1096..2082
                     /gene="cydB"
                     /locus_tag="VGA15_01935"
     CDS             1096..2082
                     /gene="cydB"
                     /locus_tag="VGA15_01935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX9586493.1"
                     /translation="MVMFWVSLLAISILLYVLLDGVDLGVGLLFGLASGEARRGAMLS
                     AVAPIWDGNETWLVVTAVILWGAFPLVYATLLSAFYLPLIIMLLGLILRGVAFEFRYK
                     TQRLRWIWDLSFAGGSLIATFIQGLMVGALVEGMRFTNGEYSGGEVGWLTPFAALCGL
                     GLCLGYALLGACWLVRKCEGEVRDVARGQIPVLAVGVLAFLVVVFAYALAENLPILHR
                     WIDRPYLFVFPAIGAVAAIVLALSILNHNDYWPFYMVALIFMSAFGTLALSFWPYMIP
                     SVITVDQAAAPQSSLTFMFWGCGVIVFPLMLIYTLVSYSVFRGKVRTTAGYH"
     gene            2173..>3189
                     /locus_tag="VGA15_01940"
     CDS             2173..>3189
                     /locus_tag="VGA15_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX9586494.1"
                     /translation="MLFNSAIWVIAAATTLGVIARPWNWPEFVWAMAGAAMLVLFNLL
                     SWQAAVAAIGRGTDVYLFLIGMMLLSEVARKEGLFNWLATQAVMHARGSAKRLFLTIY
                     VVGALVTVLLSNDATAVVMTPAVYAATRTAQVEPLPYLFICAFIANAASFVLPIGNPA
                     NLVVFGGQMPALSEWLRHFAGPSLVAILATYVALRLTQRAALDAKVAAIKSVSPLTLG
                     GKLAAIGIALTVVVLLSASAFGRPLGLPTFCAGASLTAIVLVIARQSPLPILKNISWG
                     VLPLVAGLFVLVAGLNQTVVLSALARNLHDAAAASPQAASWLAGIVVAVASNLVNNLP
                     MGLIA"
BASE COUNT          482 a          974 c          978 g          755 t
ORIGIN      
        1 ggcctcgttc ttcggcgtcc tcattttcgg ccgcagccgc gtgccgccat ggttctattt
       61 gttttcgacc gcgatggtgg cgctggggac cacgttttcg gcgttctgga tcatggtcaa
      121 caacagctgg atgcaggtgc cctcaggcta tgtgatggaa aacggcgcct tcgttccaaa
      181 cgattggacc aagatcctat tcaattcggt ggtgtggtcg cgattccccc acatgctgct
      241 cgcctcctac cttaccggcg ctttctgcgt tgccgcgacc ggtgcgtggt acatgctgcg
      301 gaaggaatat cacgccgagg cgcgtatcat gctgcgcatg ggccttggtc ttgcggctgt
      361 gctgatgccg atccagcttc tcttcgggca cctggtcggc ggctatgtca acacgtatca
      421 accttccaag atggccgcca ttgaggggcg ctggaacgac gaaaagcccg gctccgaagt
      481 gctgttcgcc tggccggatg tggagcaccg acgcaacctg ttcgcgatta cgctgccgcc
      541 gcccttcggc agcctgatcg attccgacag tcttaccgcc ggggagaccg gactcaacag
      601 catcccaccg gagaactggc cgccggtcgt catcccgttc ttcagcttcc ggatcatggt
      661 gggatgcggc atggtgatgc tggggcttgc ctgggtcggc agctatctga gttacaggca
      721 ccgcctggcg tacaatcgcc cgctgctttg gctgacgttc ctcagctttc cgctgccctt
      781 cattgcgatc attaccggct ggttcaccgc cgaagtcggc cgtcagccct gggtggtttt
      841 tggcgtgttg cgaaccgcgg atgccatgac gccgttcctg acgacgcgcg aggcgacgat
      901 ctcgctcgtg gtcttctgcg cggtctacgc cttcatcttc ctgtttggca cgttttacat
      961 ctatcggctt atccgcaccg gtccggcgag gaagccgttc gaagttccgc atctggcggt
     1021 tccgaaccgg ccgatgtcgc tggccgacga agaactaatt caagaaccca gccacgccgg
     1081 cgccggagaa taaccatggt catgttctgg gtttcgcttt tagccatcag cattctgctc
     1141 tacgtgctgc tcgatggtgt cgatctcggc gtcggcttgc tgttcggact ggcgagcggc
     1201 gaagccaggc ggggcgcgat gctgagtgcc gtcgcgccga tttgggacgg caacgagacc
     1261 tggctggtag tcaccgccgt tattctgtgg ggagccttcc cgctcgtcta tgcgactttg
     1321 ctgtcggcat tctatctgcc tctcatcatc atgctgctgg gcctgatcct gcgcggtgtg
     1381 gcgttcgagt ttcgctacaa gacgcaacgg ctccgctgga tatgggattt gagctttgcc
     1441 ggcggatccc tgatcgcgac ctttatacaa gggttgatgg tcggtgccct ggtggaagga
     1501 atgcggttca cgaacggcga atattccggc ggtgaggtcg gctggctcac gccgtttgcg
     1561 gccctttgcg gcctcggcct gtgccttggc tatgcacttc tcggcgcctg ctggctggtc
     1621 cgaaaatgcg agggcgaggt tcgcgacgtt gcgcgcggcc agatccccgt gctcgcggtc
     1681 ggcgtgctgg cgttcctcgt ggttgtcttc gcctacgcgc tggccgaaaa tcttccaatc
     1741 ctgcatcgtt ggatcgatcg gccgtatctg ttcgtattcc cggcgattgg agcagtcgcg
     1801 gcaatcgtgc tcgcgctcag catcctgaac cacaacgact actggccgtt ctatatggtc
     1861 gcgttgatct tcatgtcggc gttcggcacg ctggcgctgt ccttctggcc atacatgatc
     1921 ccctccgtca tcaccgtcga ccaagccgcc gcgccgcaat cgagtcttac gttcatgttc
     1981 tgggggtgcg gagtgatcgt ctttccactg atgctgatct acacgctcgt cagctacagc
     2041 gtcttcaggg gcaaagtcag gacaacagcc gggtaccatt aagcccgtga gcagatccga
     2101 cgcaaaccaa cacgatgcct cgcttgagca gcgaccgaca atcgccgcgg ccgtggtcgc
     2161 cagcgcaagc cgatgctgtt caattcagct atctgggtta ttgcggctgc cacaacgttg
     2221 ggtgtgatcg cccggccatg gaattggccg gagttcgtgt gggcgatggc gggcgccgcg
     2281 atgctggtgc tgttcaactt gctgtcttgg caggcggcgg tcgcggccat cggcaggggt
     2341 accgacgtct acctctttct gatcggcatg atgctgctgt ctgaggtcgc caggaaggag
     2401 gggctgttca attggctcgc gacccaggcg gtcatgcatg cgcgtggctc ggccaaacga
     2461 ctctttttga ccatttacgt cgtcggcgct ctggtgacgg tgttgctgtc gaacgacgcc
     2521 accgctgtgg tgatgacgcc ggcggtttat gcagcgaccc ggactgcgca ggtcgagcca
     2581 ctcccgtatc tcttcatctg cgctttcatc gccaacgccg caagttttgt ccttccgatc
     2641 ggcaatcctg ccaacctcgt cgtgttcggc gggcagatgc ctgctctttc ggaatggctt
     2701 cgtcattttg ccggcccgtc actcgttgcg atccttgcga catacgtcgc attgcgattg
     2761 acgcaacggg cggcgctcga cgcaaaggtc gccgcgatca agagcgtgtc cccactgacg
     2821 ctcggtggta agcttgcagc gatcggaatc gcgctgacgg tggtcgtgct gctgtcagcg
     2881 tctgcgttcg gccgaccact ggggttaccg accttttgcg ccggcgcatc cttgaccgcc
     2941 atcgtgctgg tcatcgcccg gcaatcgccg ctgccgattc tcaaaaacat atcgtggggc
     3001 gtgctgccgc tcgttgcggg tctgttcgtt cttgtcgcgg ggttgaacca aaccgttgtg
     3061 ttgtcagcgc tggcccgcaa cctgcacgac gctgcggcgg cgtcgccgca agcggcgtcg
     3121 tggctcgcag gcatcgtcgt cgctgttgcc tccaacctcg tcaacaatct gccgatgggt
     3181 ctgatcgcg
//
LOCUS       DASWGM010000059         4676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_697316,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000059 DASWGM010000000
VERSION     DASWGM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697316"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VGA15_01945"
     CDS             <1..310
                     /locus_tag="VGA15_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX9586495.1"
                     /translation="LPNAPIEVLPGKCVCEIKHSGFNKATGVLELMAHEPFRGRRPIF
                     IGDDVTDESVFALMPDLGGLAFSVGRRATGVADHFDEPRDVREWLARLLDGEKAAVRP
                     "
     gene            518..1984
                     /locus_tag="VGA15_01950"
     CDS             518..1984
                     /locus_tag="VGA15_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX9586496.1"
                     /translation="MNLVVVSNRVARGKVNEPMTGGLAAALLPVVEKSGAIWVGSSGR
                     VRDGAQKEPFAEIEALGAGALAMLDLPAAHYGGYYEGFANSALWPALHSRADLIRASQ
                     QDYLSYREVNAFMARALLRFKKPDAVFWIQDYHFLALGAELRDLGVTQPIGFFLHTPW
                     PARAIIEGVPHHRELVEAMLAYDLIGFQTEDDRESFLGYLSFDLGLEVKNGVVTSRHG
                     TTRIAVFPIGIDPERFAVQAAKAVSHPDVSRLRRSLNGEKLAIGVDRLDYSKGLINRI
                     EAFDRMWALQPALRRTVSLLQIATPSRGAIEAYGNLQNELAKLVSDVNGRHGEVDWTP
                     IRYLNKGFSQTVLSGLYRTAQVGVVTPLHDGMNLVAKEYVAAQNPADPGVLVLSKFAG
                     AANELDTALLVNPHDIDGMARTIAVALSMPLTERRMRWEAMMTKLRAGTIQQWFADFV
                     EALRETHLDKTEPEPLMAEAPPALVPLRSVNTGRARYH"
     gene            complement(1992..2300)
                     /locus_tag="VGA15_01955"
     CDS             complement(1992..2300)
                     /locus_tag="VGA15_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586497.1"
                     /translation="MSAVSAGCAGPALAQACTRQGVDVSCDDGRRGVWSGDAIIWADG
                     TRSSLASPHPSVIIGNKSSVIVGPGVFVGHGKGMVPMENPSAPNKARCAVLDGVSYCY
                     "
     gene            2538..2627
                     /locus_tag="VGA15_01960"
     tRNA            2538..2627
                     /locus_tag="VGA15_01960"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2572..2574,aa:Ser,seq:gga)
     gene            2791..4011
                     /locus_tag="VGA15_01965"
     CDS             2791..4011
                     /locus_tag="VGA15_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9586498.1"
                     /translation="MAGKLKPLEVARQITPGKYPDGDGLYLVVAGPTSRNWSYRYWIK
                     GKERWHGLGSLQDVSLKDARIKRDTARQEVRAGVDIVQVKRSAREGAKVEAAVAAAPT
                     FKECAERYIKDNESKWSKKHAAQWPSSLDQYAYPTIGHLRIAEILPSHIFELLEPIWI
                     EKRETSNRVRGRIETIIAKNVDIDDNEFRNPAELTKQLREKLPRRPKRVVRHHPALPY
                     ADAPQFMTKLSGASGIAAEMLRFVIFTACRTTEAIEARWSEIDRSLSAWRIPGERMKM
                     DQEHVVPLSDPVLAILDRMRDGSQSELIFPNPEGGQFSENAMLAVLDRLGYGHVTVHG
                     FRATFATWAEECTDYPDGVREAALAHKYKNETVAAYQRGAKLEKRRALMSDWAEFCGK
                     PATGASVVTLSRAV"
     gene            complement(4359..4541)
                     /locus_tag="VGA15_01970"
     CDS             complement(4359..4541)
                     /locus_tag="VGA15_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586499.1"
                     /translation="MIDQLTEHDRIELLARAQEAWDRAPQNTMRAVFEWRGKNYVVSH
                     SSFQLRIHTEDGIKVY"
BASE COUNT          929 a         1394 c         1440 g          913 t
ORIGIN      
        1 tctgccgaac gcgccgatcg aagtgctgcc cggaaagtgc gtctgcgaga tcaagcattc
       61 gggctttaac aaggcaaccg gcgtgctgga actgatggcc cacgaacctt tcagggggcg
      121 ccgtccgatt tttatcggcg atgatgtcac cgacgaatca gtgttcgccc tgatgcccga
      181 tcttggcggt cttgcgttct cggtgggccg gcgcgcgacg ggcgtggccg atcatttcga
      241 cgaaccgcgc gacgtgcgcg agtggctcgc gcgtctgctc gatggcgaaa aggccgcagt
      301 ccgaccatga tctgttgtgg atttgttgtg gcggccttgt ggcatcgtcg aaaccacggg
      361 gatttcgcgc gcagccagcc gttacagcga aattttctgc agccgagttc cgcccttgca
      421 ccgcccgaat ttggtttgaa ttcgttgaaa gggtgacgcg gaaccatatt gatgaatggt
      481 tgttaacttg cgagcgaaac atccaggagg ggactaggtg aacctcgtcg tcgtttcaaa
      541 ccgcgttgcg cgcggtaaag tcaatgaacc catgacgggg gggctcgcgg cggcgttgct
      601 gccggtggta gagaaatccg gcgcgatttg ggtgggttcc agcggccgcg tccgcgacgg
      661 tgcccagaag gaaccattcg ccgagatcga ggcgttgggc gcgggcgcgc tggcgatgct
      721 ggatttgccg gccgcgcatt acggcggata ttatgaaggt tttgccaact cggcattgtg
      781 gccggcgctg cattcccgcg ccgatttgat ccgcgcctcg cagcaggatt atctgtccta
      841 tcgcgaagtc aacgccttca tggcgcgggc gctgctgcga tttaagaaac ccgacgcggt
      901 gttctggatt caggactatc attttctggc gctgggcgcg gagctgcgcg atctcggcgt
      961 cacccagccg atcggattct tcctgcatac gccgtggccg gcgcgcgcca tcatcgaggg
     1021 ggtgccgcat catcgcgaac tggtcgaggc aatgctggcc tatgatttga tcggattcca
     1081 gaccgaggac gaccgtgaga gtttcctcgg ttatctttcg ttcgatcttg gtcttgaggt
     1141 caaaaacggc gtcgttacgt cacgccacgg cacaacgcgc atcgcggtat ttccgatcgg
     1201 aatcgatccc gagagattcg cggtgcaagc cgccaaggcc gtatcgcatc ccgatgtgtc
     1261 gcggttacgc cgaagcctga atggcgagaa gctggcgatc ggcgtcgatc ggctggacta
     1321 ctccaagggg ctgatcaacc gcatcgaagc attcgaccgc atgtgggcgt tgcagccggc
     1381 gctgcggcgg acggtgtccc tgctgcagat cgcaacgccg tcgcggggtg cgatcgaagc
     1441 ctacggcaat ctgcagaacg agcttgccaa gctggtcagc gatgtcaacg gccgtcacgg
     1501 cgaagtcgac tggaccccga tccgctatct caacaagggt tttagccaga ccgtgctgtc
     1561 ggggctttat cgcaccgccc aggtcggcgt ggtgacgccg ctgcatgacg ggatgaatct
     1621 cgtcgccaag gaatatgtcg cggcacaaaa tccggccgat cccggtgttc tggtgctgtc
     1681 gaaattcgca ggtgctgcga acgaactgga caccgcgctg ctggtcaatc cgcacgatat
     1741 cgacggcatg gcgcggacca tcgcggtcgc gctctcgatg cccctgaccg agcggcgcat
     1801 gcgctgggaa gcgatgatga caaaacttcg cgccggcaca atccagcaat ggtttgccga
     1861 tttcgtcgag gcgctgcggg agacccatct cgataagacc gagcccgagc cgctgatggc
     1921 tgaggcgccg ccggccctgg ttccgctgcg ctcggtcaat accggccgcg cgcggtatca
     1981 ttaatacgag gctagtagca atacgacacg ccgtcgagaa cggcacagcg tgccttgttc
     2041 ggcgcgctcg gattctccat cggcaccatg ccttttccgt ggccgacgaa tacgcccggt
     2101 ccgacgatca cagacgattt gttgccgatg atgacgctcg gatgcggcga cgccaggctc
     2161 gaccgcgtgc cgtccgccca gatgatcgca tcgccggacc agacgccgcg ccggccgtca
     2221 tcgcagctga cgtcgacgcc ttgacgcgtg caggcctgcg ccagcgccgg cccggcacag
     2281 cccgcggaga ccgcggacat gagaatcgag accacaagcg ccggatcgat ggtcatgccg
     2341 ctctccgttg gttgggttgt cgctatcgtc aatcttcgcc tgtaaccggt tttcggctca
     2401 aggctttaaa tgcgcaggcc cggcgccgcg aagaggcgtt aaattatcaa gactattcaa
     2461 tcggttgggg aaatagcccg gatcgcggtc cgccgtccct tgcaaaagtc gccgtgcccc
     2521 ttcaagacag gccatctgga gagatggccg agtggcttaa ggcgcacgct tggaaagcgt
     2581 gtgtgcggga aaccgtaccg tgggttcgaa tcccactctc tccgccagta tggtgttctc
     2641 ccttgttcgc catcattcga aacccacaat aaaatcagtg cgttgtccgg ctagttgtat
     2701 ttactcgttc gccggagttc gtcagaatcg tcgcactgtt cgtggttttt ccccgtgagt
     2761 ttggcggacg gttttgatcg gagaacacac atggctggga agctaaagcc cctggaagtc
     2821 gctcgccaga tcacgcccgg gaagtatccc gacggcgacg gcctctatct cgtcgtcgcg
     2881 ggtccgacgt caaggaactg gtcctatcgc tactggatca agggcaaaga acgctggcac
     2941 ggcctgggct ccctgcaaga cgtgtccttg aaagacgcgc gcatcaaacg cgataccgcg
     3001 cggcaggaag tgcgcgccgg cgtggatatc gtccaagtga aacgatcggc cagggagggc
     3061 gcaaaagtcg aggcggccgt tgccgcagcg cctacgttca aggagtgcgc ggaaagatac
     3121 atcaaggaca atgagtccaa gtggagcaaa aagcacgccg cgcaatggcc gtcatcgctc
     3181 gatcaatacg cctacccgac gattggtcat ttgcgcatcg cggagatttt gccgagccac
     3241 atcttcgagc tgcttgagcc aatctggatc gagaagcgcg agacttcgaa ccgcgtccgc
     3301 gggcgcatcg aaacgataat cgcgaagaat gtcgacatcg acgacaatga gtttcgcaat
     3361 cccgccgaac tcacaaagca gctgcgcgag aaacttccta ggcggccgaa gcgggtcgtc
     3421 cgccatcacc ccgccttgcc ctacgccgat gcaccgcagt tcatgaccaa gctgtcgggc
     3481 gcctcgggca tcgccgccga gatgcttagg ttcgtgatct tcacagcctg ccgcaccacc
     3541 gaggcaatag aagcgcgctg gtctgagatc gacagatctt tatcggcatg gcgaattccc
     3601 ggcgaacgaa tgaagatgga ccaggagcac gttgtccctc tgtcagatcc tgtactcgct
     3661 attctggatc ggatgcgtga cggcagccaa agtgaactga tctttcctaa tcccgaaggc
     3721 gggcagttct cggaaaacgc gatgctcgcc gttctcgatc gcttgggtta cggacacgtg
     3781 accgtgcacg gcttccgcgc cacattcgcg acctgggccg aagaatgcac cgattatccc
     3841 gatggcgtac gcgaggccgc gcttgcgcac aaatacaaga acgagacggt tgccgcttat
     3901 caacgcggag ccaagctcga gaaacggcgc gccctcatga gcgactgggc ggaattctgc
     3961 ggcaagccag cgaccggcgc ctctgtcgtg acgctttcgc gcgccgtctg atccggggcg
     4021 gaatcgaacc gctctctcca ccgtgaaaga caaaacgacc tcggtgggcg cgggacgggt
     4081 gagtagggag ccgtggttcc cggcgcgggc ggtcccgtga aggtccagcg aagccagcgc
     4141 ggccgtcgct cacttgatca ttatagcgtc cgattcgacg gtacgggagc ttggacccaa
     4201 gtttggaccc aaacaccgcg gaacacgtca taaccaatcg gcactggacg ccatcggccg
     4261 tcggcgcgac acactgaata acctacacaa aatgacggga cgatacgggc cagtatggac
     4321 cccggcgact ttcaagaccg gtgctcatgc cctatcgttc agtagacctt gatgccgtcc
     4381 tcagtgtgaa tgcgcagctg aaacgacgag tgggagacga cgtagttctt tccacgccat
     4441 tcgaaaaccg ctctcattgt gttttgcggc gcgcggtccc acgcttcctg tgcgcgtgcc
     4501 agcagttcga tccgatcatg ctcagtcagt tggtcgatca tcactcctcc gcggttccgt
     4561 gcggtcaatc cgtcatcaga tcgtaatcgc gacgcaaaac actttggaca tggagtgcgg
     4621 cgcgagccgc ggtgttggca tcagcagcgc ctgcggacaa cacacatagg gaccaa
//
LOCUS       DASWGM010000060        12752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1389981,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000060 DASWGM010000000
VERSION     DASWGM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389981"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /gene="aspS"
                     /locus_tag="VGA15_01975"
     CDS             <1..1687
                     /gene="aspS"
                     /locus_tag="VGA15_01975"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX9586500.1"
                     /translation="VADPDSPAFKDAEKLRAEWVVRIDGKVRRRPAGTENPELPTGAV
                     ELYVSEIEVLGPAGELPMPVFGEQEYPEDIRLKYRFLDLRRERLHQNIMTRGAIVDSM
                     RKRMKEQGFFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMSGF
                     DRYFQIAPCFRDEDPRADRLPGEFYQLDVEMSFVTQDDVFAAMEPVITGVFEDFAKGK
                     PVTKTWPRIPFAEALRKYGSDKPDLRNPIVMHDVSEHFRGSGFKLFAKILEDPKNQVW
                     AIPAPTGGSRAFCDRMNSWAQGEGQPGLGNIPWSLPDTLTEVGADGTNKKLPVEEGYP
                     VGRGPIANNVGPQRTAAIRDALGLKDGDAAFFVAGDPEKFWKFAGLARTKLGEELNLI
                     DKDRFELAWIVDFPMYEYNEDDKKVDFSHNPFSMPQGGLDALQTQDPLTIKAFQYDIT
                     CNGYEIASGGIRNHRPEAMVKAFEIAGYGEKDVVERFGGMYRAFQYGAPPHGGMAAGV
                     DRIVMLLCGTTNLREISLFPMNQQAMDLLMGAPSEASPKQLRELHIRTNLPNK"
     gene            1871..2239
                     /locus_tag="VGA15_01980"
     CDS             1871..2239
                     /locus_tag="VGA15_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586501.1"
                     /translation="MRILETLADISEYLDGVSYRLEDHGERWRPVVQTLVLEFMAKLS
                     PSVRLEVREWRGATGNQIGFTLRNGRRLQATYHGKLKVISIRDAVTQQTLHTYRPGSD
                     PETVADDCRRLTQPRRRLAA"
     gene            2248..3195
                     /locus_tag="VGA15_01985"
     CDS             2248..3195
                     /locus_tag="VGA15_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586502.1"
                     /translation="MPKAKREQSEHNKIESDGCTLPPDHALPPDQLGRYSPVRDFYAE
                     NDIAEYVVSQARDETVQNVERVKTEYVLGSPYEIWDVATDKDRWWVITNPTNLYSQRH
                     FPSLDYTLSFHVGLMLRVASRSERDGESEPTPFHEVFRRQAQAAKLLERAVEAVDFQA
                     VGMQLRECLISLVTAVRRRIEINGNKERPKDADVVGWNDLFAAKLCPGEKNDELRRYL
                     KATVEKVWPLVNWLTHHRNANKTAALIAKDAVGAIVVHYAHLLSRERADRTDQCPRCA
                     SRNLRTHFDIGIEPDGAYFESCGECDWDSHPGHVDDDAE"
     gene            4322..6631
                     /locus_tag="VGA15_01990"
     CDS             4322..6631
                     /locus_tag="VGA15_01990"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX9586503.1"
                     /translation="MSSHSEDLQSAALAYHRLPRPGKLEIQAIKPLANQRDLALAYSP
                     GVAAACMAIAGDPAQAASLTIRANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAVLFK
                     KFAGIDVFDIEIAADTVERVVETVAALEPTFGGINLEDIKGPECFEIEAQLKARMKIP
                     VFHDDQHGTAIIVGAAITNALLLNGKKLKDVKIVASGAGAAAIACLNLLVSLGAQRKN
                     IWVVDIDGVVHEGRNTLMDRWKSVYAQKTDKRKLSEVIGGADIFLGLSAPNVLTPDMV
                     KAMADKPLVMALANPTPEIMPDEARKARPDAMICTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATAINEDMKHAAVDAIAQLARDPPVDAIAPGFDSGEAQGFGPGSLIPSPFDPRLI
                     LRIAPAVARAAMTSGVATRPIANFEDYTAQLERFAFRSGLVMKPMFAKAKTQPVRVIY
                     AEGEDERVLHATQVVLEENLARPILVGRPSVVEARIKRFGLAIKAGQDFDLINPEDDP
                     RYRSYVQSYIDVAGRHGVTPDAARTVVRTNATVIAALAVVRGEADAMICGVEGRYMSH
                     LRHVREIVGFLPGVSDFAALALMITSKGAYFIADTQVRPNPSAEELAEMAALAALHVQ
                     RFNLKPKVAFVSHSDFGSYDTDSSRKMRRATALLKQNHPEIEADGEMQGDTALSEAAR
                     KLILPHSNLEGEANILIMPNLDTANVAYQMIKALADALPVGPILIGPSRPAHILTPSV
                     TARGVLNMTAVAAVEAQERAGRQQPTLFG"
     gene            7103..7555
                     /locus_tag="VGA15_01995"
     CDS             7103..7555
                     /locus_tag="VGA15_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9586504.1"
                     /translation="MPAFIVLGRILFAVLFIYSGATKLIGIQATTEFIASKVAIPAVL
                     VPYTSQLETMTGMSTPQTLAIVVGAFEVISGLMIALNIAARFFAILLIFFVAATIFYF
                     HDFWNQAPPDNAATLIEALKNLSIIGALFMIVGIGRLSRKAEPAYDDV"
     gene            complement(7565..8074)
                     /locus_tag="VGA15_02000"
     CDS             complement(7565..8074)
                     /locus_tag="VGA15_02000"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX9586505.1"
                     /translation="MLANAAHVMPDELKFEGDKRFFTAALDRADLIVHGRRSYEDQPN
                     SPLRKRIILTRNIGAIAPDPSNPRATLWNPAAASFEAACDAAGVRSGTVAIIGGPEVF
                     AMFMDRYDTFWLSQAPRVRLPGGEPCFPGVPARSPQAILAAHGLQAGDAEILDSADDV
                     TVTPWRRVA"
     gene            complement(8184..9125)
                     /locus_tag="VGA15_02005"
     CDS             complement(8184..9125)
                     /locus_tag="VGA15_02005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9586506.1"
                     /translation="MRIAAGLIFAGVVSLCASGTAWSQTPATKGAAALQAAPAPFPAA
                     APAPAKMPCANPNALGIARVVEIDTTGGPGFGFEHFKQLDFLRDKEVVLTFDDGPWPL
                     NTPSVLKTLADECTTGIFFSIGKHATYYPEILKQVLAAGHTIGAHTWSHATLTNKKLT
                     EQQRIEEIEKGFSAVKWALGGVSPSPFFRFPALQHPPEMVTYLGTRNIAMFSCDLDSF
                     DFKANSPKQVIDVTMKKLDKLGKGIILMHDFHKHTAEALPELLRKLKDGGYKVVQMKA
                     KAPVQTLAQYDDEILKDAKLPTVSSRPVSSVVQTISE"
     gene            complement(9299..9874)
                     /locus_tag="VGA15_02010"
     CDS             complement(9299..9874)
                     /locus_tag="VGA15_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX9586507.1"
                     /translation="MMDRRTMITAALAAIAGSGSTRTALAEPAMSRITAYAFSFPALA
                     GGDIKLAEYAGRPLLVVNTASLCGYTPQYGGLQELWSQFHDRGLMMIGVPSNDFGGQE
                     PGGATEIAETAQHQYGVTFPIAAKAVVKGPNAHPFYRWAAEARPNDVPRWNFHKYLVG
                     RDGYIAEAFPATIEPSDTRVMTAIGRALAET"
     gene            complement(9945..11594)
                     /locus_tag="VGA15_02015"
     CDS             complement(9945..11594)
                     /locus_tag="VGA15_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586508.1"
                     /translation="MNQSSTPASTTAPQIDAAKDAFDRVTLLGWRWGLAAIVGGLAAS
                     FFLFGYALIYWRNADMDFMVIYNALVLNDGKPQLFFDHTAYLTILSVKFWFQLLHALG
                     LLDAYSLATMPSAANTAAFDAAMTGAVRAGRVLAWLIATGCVLIFAGLVGYVVRDWRV
                     AMLATLAFAFSGGVAVHSRILRSELVAACPVIFALLLLIVVGRRAGVARPLAMAAAAA
                     LCVLGLENKVQAILLIGALPLVILPFGAATSASVAFWNNPRSGWMATAIAAIAAVAAA
                     SAAWPLIAIGFDRALLDAAHFHPLLLGRFGIYQAALLVLVGGCMTAFAVIWRVSATET
                     LASIFAMAAGASIALLALDLEYNTSNVIAVFNPLEKMLTFADASTTDVANGSNLFGVM
                     LLLLDGAASVLARYSFVLHSSPRPTVFLTWLIVPGIILAWRRGERQAAVQALALLLAA
                     IGIDALGVRRGLKSEYFIFTDPLIILAGAILLDSLDDLRFHKWAYPLAAILFGLHIAV
                     GQAEPIKYAFMRRGPESICEWNRAYMPLLPLPWCPLPPVRP"
     gene            complement(11658..>12752)
                     /locus_tag="VGA15_02020"
     CDS             complement(11658..>12752)
                     /locus_tag="VGA15_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9586509.1"
                     /translation="SHPLLVAQKVVPQPSVADEPKPDAPAATKTDKASDANGAVKSTI
                     SEASLELRPTVGHDENVKPVMRESSASTPLHDRTRTADASNGLPAANGPVTMTDATSS
                     AGNASPRGEPAARTDSAPPDTASVKADEAAEPSTAKRSDGASSDDKPVETKPPAAAVA
                     TEGAKAQDGKAEAKAEATRVEVKADRARTEVPKAKDQTRLDTPRPASTMGAAPKRSVQ
                     ISVFVSRKDSKLYARQNFAPLFDVPVTIAPGDRPLGTHVFTAQVDKDDANVLHWSVVS
                     LPMRARYAEQRDEDERGSRRRRVAGAAEIKPMPVADSPAEALDRLTIPAEAMARIAEA
                     LSTGGSIIVSDQGIAAGETGEGTDFIVSLR"
BASE COUNT         2458 a         3842 c         4022 g         2430 t
ORIGIN      
        1 cgtggccgat ccggattcgc cggcgttcaa ggacgccgaa aagctgcgcg cggagtgggt
       61 ggtgcggatc gacggcaagg tgcgccgccg tcccgccggc accgagaatc cggaattgcc
      121 gaccggcgcg gtcgagcttt atgtgagcga gatcgaggtg ctgggaccgg ctggtgaact
      181 gccgatgccg gtgttcggtg agcaggaata tcccgaggac ataaggctta agtaccgctt
      241 cctcgacctt cgtcgcgagc ggctgcacca gaacatcatg acccgcggcg cgatcgtcga
      301 ttccatgcgc aagcgtatga aggaacaggg cttcttcgag ttccagaccc cgatcctgac
      361 ggcgtcgtcg ccggaaggcg cgcgcgactt cctggtgccg tcaaggattc atccgggcaa
      421 attctacgcg ctgccgcagg cgccgcagca atacaagcag ttgctgatga tgtccggctt
      481 cgaccgctac ttccagatcg cgccatgttt ccgcgacgaa gacccgcgcg ccgataggtt
      541 gcccggcgag ttctatcagc tcgacgtcga gatgagcttt gtcacgcagg acgatgtgtt
      601 cgcggcgatg gagccggtta ttaccggcgt gttcgaggat ttcgccaagg gcaagcccgt
      661 caccaagacg tggccgcgga ttccgttcgc cgaggctttg cgcaaatacg gcagcgacaa
      721 gcccgatctg cgcaatccga ttgtcatgca cgacgtctcc gagcacttcc gcggctccgg
      781 cttcaagctc tttgcaaaaa ttctggaaga cccgaagaat caggtctggg cgattccggc
      841 accaactggc ggaagtcgcg cattctgcga ccggatgaat tcctgggcgc agggcgaagg
      901 ccagccgggg ctcgggaata ttccgtggag cttgcccgac acactcacgg aagtcggggc
      961 agatggcaca aacaaaaagc ttccggtcga agagggctat ccggttggcc gtggcccaat
     1021 agccaacaac gtcggaccgc agcgcaccgc cgcgattcgt gatgcgttgg gcctaaagga
     1081 cggcgatgcc gcgttcttcg tcgctggcga tccggaaaaa ttctggaaat ttgccggatt
     1141 ggcgcgaacg aaactcggcg aggagttgaa cctgatcgac aaggatcggt tcgaactggc
     1201 ctggatcgtt gactttccga tgtacgaata caacgaggac gacaagaagg tcgacttctc
     1261 gcacaatccg ttttcgatgc cgcagggcgg tctcgatgcg ttgcaaactc aggatccgct
     1321 gaccatcaag gcgtttcagt acgacatcac ctgcaacggc tacgagattg cctccggcgg
     1381 tatccgcaat catcggcccg aggcgatggt gaaggcgttc gagatcgcag gctatggcga
     1441 gaaggatgtc gtcgaacgtt ttggcggcat gtaccgcgct ttccagtatg gcgccccgcc
     1501 gcatggcggc atggcggccg gcgtcgatcg cattgtcatg cttctctgcg gcactacgaa
     1561 cctgcgtgaa atctcgctgt tcccgatgaa ccagcaggcc atggacctgc tgatgggcgc
     1621 gccttcggaa gccagcccga agcagttgcg cgagcttcac atccgaacca atttgccgaa
     1681 caagtagttc ggcagcgaag acgaggcgtc gatccgctgc ctgcgggacc tatgcgcgcg
     1741 tactcacgac acggcggagc gatggcccgc gctgccgcag actgccggca ccaaattttc
     1801 cgccgaagct tgcaagttct gtagcccgcc accatgttcc gctgatcttt tgcagcacca
     1861 ggagaacggg atgcgaatcc tcgaaacttt ggcggacatc agcgagtacc ttgatggcgt
     1921 ctcctaccgc ttagaagatc acggcgagcg ttggcgaccg gtggtgcaaa ccctcgtgct
     1981 cgaattcatg gccaagttgt cgcccagcgt gcggttagag gttcgagagt ggagaggcgc
     2041 aactggtaac caaatcggct tcacgctcag aaacggccgt cgcttgcaag caacatatca
     2101 cggtaagtta aaagtcatta gcattcggga tgccgtgacc cagcagacgc tgcacaccta
     2161 taggcctggc agtgaccccg agactgtcgc cgacgattgc cgccggctga cacagccgcg
     2221 gcgccgtctc gccgcttaaa tctgccgatg ccaaaggcca agcgcgagca atcggagcac
     2281 aacaaaatag aaagcgacgg ctgcacgctg ccgccggatc acgcactgcc gcctgatcaa
     2341 ttgggccgtt acagtcctgt gcgtgacttt tacgcagaga acgacatcgc ggagtacgtg
     2401 gtgagtcagg cacgcgacga gacggtccag aacgtcgagc gcgtcaaaac cgaatatgtg
     2461 cttggctcgc catacgagat ttgggacgtt gccacggaca aggatcgttg gtgggtgatc
     2521 acgaatccga caaaccttta ttcccaacgc cattttccaa gtctcgatta tacgctgtcc
     2581 tttcacgtcg gactcatgct gcgcgtggca agccgctccg agcgtgatgg cgagtccgag
     2641 cccacgccat ttcacgaagt ttttcggcga caagcgcaag ctgccaagtt gctggagcgc
     2701 gcggtggagg cagtcgattt tcaagctgtc ggcatgcagc ttcgcgagtg tctaatctcc
     2761 ctggttaccg ccgtgcgccg ccgcattgag atcaatggca ataaagaacg gcccaaagac
     2821 gctgacgtag tgggctggaa cgacctcttt gcagcgaagc tttgtcctgg cgaaaagaat
     2881 gatgagttac gtcgatatct aaaggcgacc gtcgagaaag tctggcccct ggtcaattgg
     2941 ctcacccatc atcgcaacgc gaacaagacg gctgctttga tcgcgaagga cgccgtcggg
     3001 gcgattgtag tgcactacgc gcacctgctc agccgcgagc gcgccgaccg taccgatcaa
     3061 tgtccgcgct gcgcttcgcg caatttgcgc acgcatttcg atattggcat tgaaccggac
     3121 ggtgcctatt ttgagtcctg cggcgagtgt gattgggaca gccatccggg ccatgtcgat
     3181 gatgacgcgg agtgacccag gaaagcgatc gggggtcgag cgccgatagt ctgcttaggg
     3241 tcgatactgt tgaaaaagtc gaaaatcgag ggcttcgaaa atctcgcgaa tgtcgaatgt
     3301 tgacgatctc agccgctgca aggttctgca gaaccgatac gagcgtcagc ggtcgctttt
     3361 gcgttaatcg gtgtggtccc tcacatcgca gcgcgtgaga cgcaggagcg gtcctgggaa
     3421 ttttcggtca tcagctaaaa aggacttttt caacacaatc ggtcaactgc agacgttcga
     3481 cggtttcgtt ggcacgcact tgcccgacgg gcatttcccg catgcacgcc atgcgcgaac
     3541 tgcccgtcgt gccaatctgc cgcatgggtt cgcgcttgcg gcgtcgggca aatcagaacc
     3601 atcttctcgc gcatcccgcc tcgatgaaga gggacgtacg cgcgttcgtc acgatacgtg
     3661 aggcggggat gcgatggacg cggatggcac gtgagacgaa cgtggcaaac gcggacggcg
     3721 aagtcgtatg gtcctggcct cccgacgctg aggctaaggc cgcgagatca tctcgcggac
     3781 gacgggggca agaaagcccg gtccccgggg agagtacgaa ggaaaccgtt aaaaccatcg
     3841 cgcagggaat gccgggttga tctggctaaa cctgtggtga ctgccgcctg ctttttttgt
     3901 tgcaggcggg ccatgggtga ggccatcacc cggcattccc tgcgccctct tcgatcttcg
     3961 agggccaagt tacttgcaac actcgggcgt gaacgcgtcg cgggatcgcg tcgatgtgtt
     4021 cacctcaagg tggtataatt ttagtgtcgc tatttgaggg cgcggccggg ccgccaagcc
     4081 tggtgcggtc ggcttggcag ctatccgcag accggcccgg attgctgtgg ggccatggcc
     4141 attgtcacaa ttgctgaggg tagggaggtg tctggcgcac cacgattatt ccccccggag
     4201 gctgttgatg tgagccgttt cagatgttgc gcgaaggcac ctgtgcgtgc ttcacatccg
     4261 aaccaatttg ccacagaact gacgccgccc ctgtcaagca atcggggccg ggggagcacg
     4321 tatgtcgtcc cattctgaag atctccaatc cgcggcgctc gcctatcacc ggttgccgcg
     4381 gccgggcaag cttgagattc aggcgatcaa gccgctcgcc aaccagcgcg atctcgcgct
     4441 ggcttattcg ccgggggtgg ccgcggcctg catggcgatt gccggcgatc cggcgcaggc
     4501 ggcctcgctg acgatccggg ccaatcttgt ggcggtggtc tccaacggca ccgcggtgct
     4561 cgggcttggc aatatcggcc cgctggcgtc gaagccggtc atggagggca aggcggttct
     4621 gttcaagaag ttcgccggca tcgacgtgtt cgacattgaa atcgccgccg acacggtcga
     4681 gcgcgtggtc gagaccgtgg ccgcgctgga gccgaccttc ggcggcatca atcttgagga
     4741 catcaagggt ccggaatgtt tcgagatcga ggcgcagctc aaggcgcgca tgaagatccc
     4801 ggtatttcac gacgaccagc acggcaccgc catcatcgtc ggtgccgcga tcaccaatgc
     4861 gctgctgctc aacggcaaga agctcaaaga cgtcaagatc gtcgcctccg gcgccggcgc
     4921 cgcggcgatc gcctgtctca atcttctggt ctcattgggc gcgcagcgca agaatatctg
     4981 ggtcgtcgat atcgacggcg tggtgcacga aggccgcaac acgctgatgg atcgctggaa
     5041 gtcggtctat gcgcagaaga ccgacaagcg caagctttca gaagtgatcg gcggcgccga
     5101 catttttctc gggctgtcgg ctcccaacgt gctgacaccg gacatggtca aggcgatggc
     5161 cgacaagcca ttggtgatgg cgctcgccaa cccgacgccg gagatcatgc cggatgaggc
     5221 ccgcaaagcg cggcccgacg ccatgatctg caccgggcgc tccgacttcc ccaatcaggt
     5281 caacaacgtt ctgtgctttc cgttcatctt tcgcggcgcg ctcgacgtcg gggccaccgc
     5341 gatcaatgag gatatgaagc acgccgcggt cgacgcgatc gcgcagctgg cgcgcgatcc
     5401 gccggtggat gcgatcgcgc ccggtttcga cagcggcgaa gcgcaagggt tcggtccggg
     5461 ctcgctgatt ccgagcccgt tcgatccgcg cctgatcctg cgcatcgcgc ctgcggttgc
     5521 aagagcggca atgacgtcgg gcgtcgccac gcggccgatc gcgaatttcg aggactatac
     5581 cgcgcaactg gagcgcttcg cgttccgctc cggccttgtc atgaagccga tgttcgccaa
     5641 ggcgaagacc cagccggtcc gtgtgatcta tgcggaaggc gaggacgaac gcgtgctgca
     5701 cgcgacgcag gtcgtgctgg aggagaatct ggcgcggcca attctggtcg gacgcccctc
     5761 ggtggtcgaa gcgcggatca agcggttcgg tctcgccatc aaggccggcc aggatttcga
     5821 tctcatcaat cccgaggacg atccgcgcta tcgatcctat gtccaatcct acatcgacgt
     5881 cgccggacgg catggcgtga cgcccgacgc cgcgcgcacg gtggtgcgca ccaacgccac
     5941 cgtaatcgcg gcgctcgcgg tggtgcgcgg cgaagccgac gccatgatct gcggggtcga
     6001 aggccgctat atgagccatc tgcgccacgt gcgcgagatc gttggcttcc ttccgggcgt
     6061 gagtgatttc gcagcattgg cgctgatgat caccagcaag ggcgcctact tcatcgccga
     6121 tacccaggtg cggccgaacc cgagcgccga ggagcttgcg gagatggcgg cgctggcggc
     6181 gctccatgtc cagcgcttca atctcaagcc caaggtcgcg ttcgtgtcgc actccgattt
     6241 cggcagttac gataccgact cctcgcgcaa gatgcgccgt gccaccgcgc ttctgaagca
     6301 aaaccacccg gagatcgaag cggacggtga aatgcagggc gataccgcgc tgtcggaagc
     6361 ggcaaggaag ttgatcctgc cgcattcaaa tcttgaaggc gaggccaata tcctgatcat
     6421 gccgaacctt gataccgcca acgtcgccta tcagatgatc aaggcgctgg ccgatgcgct
     6481 tccggtaggg ccgatcttga ttgggccctc gcgtccggcg catattctca cgccttcggt
     6541 gaccgcacgc ggcgttctca acatgaccgc ggtggccgcg gtcgaagccc aggaacgcgc
     6601 cggccgccag caaccgaccc tgtttggcta ggctcgcacg tattccgaca agcctgccga
     6661 ggacggatac cggcgattgg caagcagatt tgtgcagatt gcgctgcggc agaagtagac
     6721 ttatctgcgg tcgaatacgc gcaagatctt ggtcaggggc ccttttgttg agttagcccg
     6781 attccacctg accgaaaaat gcactagtgt gacagtttgg aagctctctt cattgttgca
     6841 gcgcggccgc atgggaggac ttcttagcca aagccacact agcatcataa tttccggtgt
     6901 tctgtcgaag ccgaagttcg cagcacaagg atgctgcggt gatgcggact tcgaattctg
     6961 cacgttcgtg aaaatccgat cgagttcccg tcgcggcgac cgcgcattcg aaaagatttg
     7021 atagtttgca acctgaccgg aatgcccgta accgctccct cctgcagcaa ccccgcatca
     7081 gcagcattca atgaggccga ccatgccagc gtttattgtt ttagggcgca ttctgtttgc
     7141 cgtgctcttt atctactcgg gagcaaccaa gttaatcggc attcaggcca ccacggagtt
     7201 tatcgcatcc aaggttgcaa ttccggctgt gcttgttccc tacacgtcgc agttggagac
     7261 gatgacggga atgtcgacgc cgcaaacgct cgccatcgtc gtcggagcct tcgaggtcat
     7321 cagcgggttg atgatcgcgc tcaatatcgc tgcgcggttc tttgctattc tgttgatctt
     7381 ctttgtggct gcgacaattt tctatttcca cgatttctgg aatcaggcgc cgccggacaa
     7441 tgccgcgacc ctgatagagg cgctgaagaa tctgtcgatc atcggcgcgc tgttcatgat
     7501 cgtcggaatt ggccggttgt cgcggaaggc ggagcccgcc tatgacgatg tctaagccgg
     7561 acgtctaagc cacccgccgc catggggtga cagtgacgtc atcggcgctg tcgaggatct
     7621 cggcatcgcc ggcctgcagg ccgtgcgccg ccaggatggc ctgtggcgaa cgtgcaggga
     7681 ctccgggaaa gcacggctcg ccgcccggca gccgcacccg gggcgcctgc gagagccaga
     7741 acgtgtcgta gcgatccatg aacattgcga acacctccgg tccgccaatg atcgcgacgg
     7801 tgccggaacg cacgccggcg gcatcgcagg ccgcttcgaa cgaagccgcg gccggattcc
     7861 acagggtggc cctggggttg gaaggatcgg gcgcgatcgc gccgatgttg cgggtcagga
     7921 tgatccgctt tcgcagcggt gagttcggct ggtcctcata ggaccgccgg ccatggacga
     7981 tgaggtcggc gcgatcgagc gcggccgtga agaaccgctt gtcgccctcg aatttcagtt
     8041 cgtcgggcat cacatgcgcg gcattcgcca gcatgccgtc agccgacacg atgacatagc
     8101 cgccaatgcg cagagctgga cgcagacttg agcgcagact tgagcgcaga cttgagcgca
     8161 gacttggcac cgcgcgggcg aatctattcc gaaatcgtct gcacgacgct ggagacgggc
     8221 ctggaactca cagtcggcaa tttggcgtct ttgaggattt cgtcgtcgta ctgcgcgagg
     8281 gtctgcaccg gagccttggc cttcatctgc acgaccttgt agccgccgtc cttcagtttg
     8341 cgcagcagtt cggggagggc ctccgccgtg tgcttgtgga aatcatgcat caggatgatg
     8401 cctttgccga gcttgtcgag cttcttcatc gtgacgtcga tcacctgttt cggcgagttt
     8461 gccttgaaat cgaacgagtc gaggtcgcag gagaacatag cgatgtttct ggtgccgaga
     8521 taggtcacca tttcaggcgg atgctgcagc gccggaaagc ggaagaatgg cgacggggaa
     8581 acgccgccca gcgcccattt caccgcgctg aaacctttct cgatctcctc tatgcgctgc
     8641 tgttcggtca gtttcttgtt ggtcagggtc gcgtgcgacc aggtgtgggc tccgatggtg
     8701 tggccggccg cgagtacctg tttcaggatt tccggatagt aggtcgcgtg cttgccgatc
     8761 gaaaagaaaa tgccggtcgt gcattcgtcc gccagcgtct tcagcaccga gggtgtattg
     8821 agcggccacg ggccatcgtc aaacgtcagc accacttcct tgtcgcgcag gaaatcgagt
     8881 tgcttgaagt gctcgaaacc gaatcccggt ccgccggtgg tgtcgatttc gacgacgcgc
     8941 gcgatcccaa gcgcattggg gtttgcgcaa ggcatttttg cgggagccgg ggctgctgcg
     9001 gggaagggcg ctggagcggc ttgcagggct gccgcgccct tggtggccgg ggtctgtgac
     9061 catgccgtgc ctgacgcaca cagcgaaacc acgcctgcaa agatcaaacc tgccgcaatg
     9121 cgcatcatat ttccccttat gctgatccgc gtgccggcct cggctttcgc tttcgccgga
     9181 gcggttttct gcccctgttg acaatagcct agtaggacct gcgccgccac ggcaaaggct
     9241 tttgttggcg gccgccattt tgtcggtcga acgtgttaat tccgggggag accgcgcgtc
     9301 aggtctccgc aagcgcccgc ccgatggcgg tcatgactcg cgtatccgat ggctcgatgg
     9361 tcgccgggaa cgcctcggcg atgtagccgt cgcgcccgac cagatatttg tgaaagttcc
     9421 agcgtggaac atcgttcggc cgtgcctccg cggcccatcg gtagaacgga tgcgcgttcg
     9481 ggcctttgac gacagctttg gccgcgatcg gaaaggtaac gccatattgg tgctgtgcgg
     9541 tctcggcgat ttcggttgcg ccgccaggct cctgaccgcc gaaatcgttg gagggaacgc
     9601 cgatcatcat caagccgcgg tcgtgaaact ggctccacaa ttcctgcaat ccgccatatt
     9661 gcggcgtgta accgcacaat gacgccgtgt tgacgaccag cagcggacga ccggcatact
     9721 cggcgagttt gatatcgccg ccggccagcg caggaaacga aaaagcataa gcggtgattc
     9781 ggctcatggc aggctctgcc agagcggttc gtgtcgagcc cgaacccgcg atagcggcca
     9841 gcgccgccgt gatcattgtc ctgcgatcca tcatagccac ctcgggcgca attcaggtgc
     9901 ggcagaatag cccaaccggc gcgcggccgc cgctcaacaa gccgttatgg ccggaccgga
     9961 ggaagggggc accacggcag cggcagcagt ggcatgtaag cccggttcca ctcgcatatc
    10021 gattccggcc ccctgcgcat gaaggcgtat tttatcggct cggcctggcc gacggcaata
    10081 tgcagcccga acaggatcgc ggcgagggga taggcccatt tgtggaagcg cagatcgtca
    10141 agcgaatcga gcaggattgc gcctgcgagg atgatcaggg gatcggtgaa gatgaaatat
    10201 tcggatttca atccgcgccg cacgcccagc gcgtcgattc cgatcgcggc cagcagaagc
    10261 gcaagcgcct ggaccgcggc ctgcctttcg ccgcgtcgcc acgccagaat gatgccgggc
    10321 acgatcagcc aggtcagaaa caccgtgggg cgcggtgagg agtgcagcac gaagctgtag
    10381 cgcgcgagaa ccgaagccgc cccatcgagc agaagcaaca tcactccgaa cagattggat
    10441 ccgttggcga catccgtggt gctggcatcg gcaaaagtca gcattttttc gagcggattg
    10501 aatacggcaa tcacattgct ggtgttgtat tcgagatcga gcgccagcag cgcaatcgac
    10561 gcgccggcgg ccatcgcaaa tatcgacgcc agcgtttcgg tggcgctgac gcgccagatc
    10621 acggcaaagg cggtcatgca tccgccgacc aggaccagca atgcggcctg atagatgccg
    10681 aagcgaccca gcaacaatgg atggaagtgc gcggcgtcga gcaaggcgcg gtcaaagccg
    10741 atcgcgatca gcggccacgc agccgatgcc gccgcaactg ccgcgatggc cgcgatagca
    10801 gtcgccatcc agcctgatcg tggattgttc cagaacgcga cgctggcgct tgtcgcagct
    10861 ccgaacggca ggataaccag cggcagcgcg ccgatcagca aaatggcctg caccttgttc
    10921 tcaagaccca gcacgcacaa ggccgccgcg gccgccatcg ccagcggacg ggcaacgccg
    10981 gcgcggcgtc cgaccacgat cagcagcaac agcgcaaaga tgacgggaca ggccgcgacc
    11041 agctcgctgc gcaaaatgcg cgagtgcacg gcgacgccgc cggagaaggc aaaggcaagc
    11101 gttgcgagca tggcgacgcg ccagtcccgc accacgtagc caacaaggcc tgcaaaaatc
    11161 agcacgcaac cggtcgcgat cagccaggcg agaacacgcc cggcgcgaac ggcgccggtc
    11221 atggcggcat cgaatgccgc ggtgttggcg gccgacggca tggtggccag cgaataggcg
    11281 tcgagcaggc cgagcgcgtg cagcagctgg aaccagaact tgaccgacag gatggtcaga
    11341 taggcggtgt gatcgaagaa cagctgcggc ttgccgtcgt tgaggacaag ggcgttgtag
    11401 atcaccatga aatccatgtc ggcgttgcgc caatagatca aggcgtagcc gaacagaaaa
    11461 aacgacgccg cgaggccgcc aacgatggcc gccagtcccc atcgccagcc aagcaacgtc
    11521 acccggtcga aggcatcctt cgctgcgtca atctgcggcg cggtcgtgga ggccggagtg
    11581 gaggactggt tcatgccggc cggtgtttta gcttagccgc cggtgcaaag cgacggcggc
    11641 tgaaaggcca cggccgtcta gcgcagcgaa acgatgaaat ccgttccttc gccggtttcg
    11701 ccggcagcga tgccttgatc ggaaacgatg atcgagccgc cggtcgaaag cgcctcggca
    11761 attcgtgcca tcgcttccgc cgggatggtg aggcgatcca gcgcctcggc cgggctgtcc
    11821 gccaccggca tcggcttgat ctcggccgcg cccgctaccc tgcgccgccg cgacccgcgt
    11881 tcgtcttcgt cgcgctgttc ggcgtaccgc gcccgcatcg gcagcgagac caccgaccaa
    11941 tgcaggacat tggcgtcgtc cttgtcgacc tgggcggtga acacgtgggt tccaagcggc
    12001 cggtcacccg gcgcgatcgt caccggcacg tcgaacagcg gcgcgaaatt ctgccgcgca
    12061 tagagttttg aatccttgcg gctgacgaac accgagatct ggacgctgcg cttcggcgct
    12121 gcacccattg tcgaggcggg tcttggggtg tcgaggcgag tctgatcctt tgccttcggg
    12181 acttccgtcc tggcgcgatc cgcctttact tcgaccctgg tcgcctcagc ctttgcttcg
    12241 gctttgccgt cttgggcctt cgcgccttcc gttgcaaccg cggctgcagg cggtttggtc
    12301 tcgaccggtt tgtcgtcgga agatgcccca tcgcttctct tcgcggtgga cggttcggct
    12361 gcttcgtcag ccttgacgga ggcggtgtcc ggcggcgcgc tgtcggttcg ggcggcgggc
    12421 tcgccgcggg gtgacgcatt gcccgcggag gatgttgcat cggtcatcgt gaccgggccg
    12481 ttggcggccg gcaagccgtt gctggcatcc gcggtgcggg tccggtcgtg caacggcgta
    12541 gacgccgacg attcgcgcat gaccggcttc acgttctcgt cgtgtccgac cgtcggtctc
    12601 agttccaggc tcgcttccga aatcgtggac ttcaccgccc cattcgcgtc cgacgccttg
    12661 tcggttttcg tggccgcagg cgcatccggt ttcggctcgt cggcgacact cggctgcggc
    12721 acaaccttct gtgccaccag tagcggatgc ga
//
LOCUS       DASWGM010000061         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1390062,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000061 DASWGM010000000
VERSION     DASWGM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390062"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /gene="bamD"
                     /locus_tag="VGA15_02025"
     CDS             complement(<1..454)
                     /gene="bamD"
                     /locus_tag="VGA15_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX9586510.1"
                     /translation="MSAQRMTLELRKVPDGAGFRRIGRQLRLAAGLIVLALPLSGCGT
                     GALWDKFMAKDDTFVEEPADKLYNEGLYLMIQRKDPKAASKKFEEVDRQHPYSDWARK
                     SLLMSAYAFYNSGDYDSCIGAATRYVTLHPGSPDAAYAQYLIAASHYDQ"
     gene            complement(852..1811)
                     /gene="lpxC"
                     /locus_tag="VGA15_02030"
     CDS             complement(852..1811)
                     /gene="lpxC"
                     /locus_tag="VGA15_02030"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX9586511.1"
                     /translation="MKFSRQTTLRSQATVTGVGVHSGLPVSLTLGPAPVDAGFIFVRT
                     GLDGSDREVQANAKSVIATELATVLGDHDGPLVSTAEHVLAALRGMGVDNAIIEVDGP
                     EVPIMDGSAAAFVAAIDQAGIVEQPARRRFIQVLRPVQVAMGESFGELRPYANGFRAE
                     VEIDFTNPVIGRQSFVLDLHPESFRRDISRARTFGCMNDVARLWSAGFSLGASFENTV
                     VFDETRLLNTEGLRYSDECARHKVLDVVGDLALAGLPLLGAYHSVRGGHKLNHAVLTA
                     LLADRSAWRVIEAETARRSRTHAEVGSGRVGGMIAPAYGPDVS"
     gene            complement(2163..>3545)
                     /locus_tag="VGA15_02035"
     CDS             complement(2163..>3545)
                     /locus_tag="VGA15_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9586512.1"
                     /translation="PFHFEGQRRMRTAEAGIAELHKVVDTLLIIPNQNLFRVANEKTT
                     FADAFAMADQVLYSGVACITDLMVKEGLINLDFADVRAVMREMGKAMMGTGEASGEKR
                     ALTAAEAAIANPLIDDSSMKGARGLLISITGGRDLTLFEVDEAATRIREEVDQDANII
                     VGATFDENLDGVIRVSVVATGIEQALISRNANPTAAATTVSATPDNRLADLTARLRAD
                     NQRLAERAQKLDPSAAAQIPSAALQQRASSGVERAALAAIAAAVAPESVQPAASAPLQ
                     PASYGDVTVRPIGIAQKPTLFPDHDTGRVEAHQSPTPETFIPQAAERAPVRAPRMPKF
                     EELPVPAQNEIRQARGEVEDDHPQKTRMSLLQRLANVGLGRRDEETEPPIAARASGPA
                     MAPMPPLAERKPQRSVAQQITAGDPVSEYARRPAPQGLDVHGRPAPVASVPQGDDHLD
                     IPAFLRRQNN"
BASE COUNT          690 a         1080 c         1112 g          663 t
ORIGIN      
        1 tctggtcgta atgcgaggcg gcgatcaggt attgcgcata ggcggcgtcg gggctgccgg
       61 gatgcaaggt gacgtagcgg gtcgcagcac cgatgcagct gtcgtagtcg cccgaattgt
      121 aaaaggcgta ggccgacatc agcagcgatt tgcgcgccca gtcggaataa ggatgctggc
      181 ggtcgacttc ctcgaatttc ttcgatgcgg ccttcggatc cttcctttga atcatcaggt
      241 acaagccctc attatagagc ttgtcggcgg gttcctcgac aaaggtatcg tccttggcca
      301 tgaacttgtc ccacagcgcg ccggtgccgc agccgctaag ggggagcgcg agaacaatga
      361 gaccggcggc gagccgcagc tgccggccga tccggcggaa gccggcgcca tccggtacct
      421 tgcgcagttc gagcgtcata cgctgtgccg acatgaatct gagacctgat gcctgtgatg
      481 cggtgcccgc cacgccacga acgcggtcat ggacgcgaaa acccatgcct gtggcgcttg
      541 tcgtgcaacc gcgggcctgt gtgtagctca taaaagtccg ttaaccaacc gaatacgcag
      601 gcatttcgcc cggattaagg cgggtaggat tggattcggg ccgatcatgg ttaccgcagc
      661 aaattgagcg gcaaatcgcc tccggcgacg ctcaggagac gttcaaggaa cgctcgggag
      721 acgtcaagga acgctcggga gacgtgaagc tgatggccag aacatttcga agacgaaagt
      781 caggacaaaa gtcaggtgac gtccagaaac gctcaggaca cgaaacgctc aggacatttc
      841 gcaagcgaaa gtcaggacac gtcggggccg taggccgggg cgatcatgcc gcccaccctt
      901 ccactgccga cttcagcatg cgtgcgcgaa cgacgcgccg tttccgcctc gatcacccgc
      961 caggcgctgc gatcggccag cagggcggtc agcacggcgt gattgagctt gtggccgccg
     1021 cgcaccgagt gataggcgcc aagcagcggc aggccggcca gcgcaagatc gccgaccacg
     1081 tcgagcacct tgtggcgcgc gcattcatcg ctgtagcgta gcccttcggt attcagcaac
     1141 cgggtctcat cgaacaccac ggtattttca aacgacgcgc caagtgagaa gcccgcgctc
     1201 cagagccgcg ccacatcgtt catgcatccg aaggtacggg cgcgggaaat gtcgcggcgg
     1261 aaactttccg gatgcaggtc cagcacaaaa ctctgacgac cgattaccgg attggtgaaa
     1321 tcgatttcga cttcggcgcg gaagccgttc gcatagggcc gcagttcgcc gaacgattcg
     1381 cccatcgcga cctgcaccgg cctcagcacc tggatgaagc ggcggcgcgc cggctgttcc
     1441 acgataccgg cctgatcgat cgcggcgacg aaagcagcag cgctgccatc catgatcggt
     1501 acttcgggac cgtcgacttc gatgatggcg ttgtcgacgc ccattccgcg caacgcagcg
     1561 agaacgtgtt ccgcagtgga taccagtggg ccgtcgtgat cgcccagcac ggtggcaagt
     1621 tcagtcgcga tcactgattt ggcgttggcc tgcacttcac gatcggaccc atccagaccg
     1681 gtgcggacaa aaataaaacc tgcatccacg ggcgcaggtc caagagtcaa acttaccggt
     1741 aaacccgaat gaacaccgac acctgtcact gtggcttgcg atcgaagcgt tgtctgccgg
     1801 ctgaatttca tttcgaaagc tgcccactac cgacttatct aaagaccaga tccccccagt
     1861 tgggcgggcg aatcacgctt cagatcccca aagtcacggc aaaccatagt cagcgcccca
     1921 aagcgcgcca actcacgctt ttttaccgat tgttacccta tctctgccgt attcgcccca
     1981 aatttaacta aattgggcca cggttaacgc caaattcgac ttaggcagct caacctaaaa
     2041 ttcataattt agtgtttaaa atcaattgct tggtctattt tccgcgacat tctcgcggcc
     2101 aaaaacaaag gccccgactt gcgaagccgg ggcctttttc gtcgtcagaa ttgtaacaaa
     2161 cctcagttgt tctgccgccg caggaaggcg gggatatcaa gatggtcgtc gccctgtggc
     2221 actgaggcaa caggcgcggg gcggccatgg acgtccaatc cctgcggtgc cggccgcctt
     2281 gcgtattctg ataccggatc accggccgtg atttgctgcg cgaccgagcg ctggggcttg
     2341 cgctccgcga gcggcggcat gggggccatg gccggacccg aggcgcgggc cgcgatcggc
     2401 ggctcggtct cctcgtcgcg acggccgagg cccacattgg caaggcgctg cagcagcgac
     2461 atccgggtct tttgcggatg gtcgtcctcg acctcgccgc gggcctgccg aatttcgttc
     2521 tgcgcaggta ccggcagctc ttcgaatttc ggcatccttg gcgcgcggac cggggcgcgt
     2581 tcggccgcct gcgggatgaa tgtctcgggc gtcggggact ggtgggcctc gacacgcccg
     2641 gtatcatgat ccggaaataa tgtcggcttc tgtgcgatcc cgatcggacg aacggtgaca
     2701 tcgccatagg acgcaggctg cagcggtgcg gatgccgccg gctgcacgga ttccggagca
     2761 acagccgcgg cgatggccgc cagcgccgcg cgttcgacgc cgcttgaggc gcgctgctgc
     2821 agtgcagccg aaggaatctg cgctgcagcg gacggatcaa gtttctgggc gcgttcggcg
     2881 agacgctgat tgtcggcgcg cagccgtgcc gtgaggtcgg ccaggcggtt gtccggcgtc
     2941 gcgctcacgg tcgtggcggc tgccgtcggg tttgcgttgc gcgagatcag cgcctgttcg
     3001 atgccggtgg caaccacgga aacccgaatc acgccatcga ggttctcgtc gaaggtggcg
     3061 ccgacgatga tattggcgtc ctgatcgacc tcctcgcgaa tccgggttgc ggcttcgtcg
     3121 acctcgaaca gggtcagatc ccggccgccg gtgatcgaga tcaagaggcc gcgggcgccc
     3181 ttcatcgacg aatcgtcgat caacggattg gcaatcgcgg cttcggccgc ggtcagcgcg
     3241 cgcttttcgc cggaggcctc gccggtgccc atcatcgcct tgcccatttc gcgcatcacg
     3301 gcgcgaacgt cggcgaagtc gagattgatc aggccttcct tgaccatcag atcggtgatg
     3361 caggcgacac ccgaatacag cacctggtcg gccatcgcga aggcgtcggc gaaggtggtc
     3421 ttttcgttgg cgacccggaa caggttctgg tttggaatga tcagcagcgt atcgaccacc
     3481 ttgtggagct cggcgatgcc ggcctcggcg gtgcgcatgc ggcgctggcc ttcgaagtgg
     3541 aacgg
//
LOCUS       DASWGM010000062        10476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2221703,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000062 DASWGM010000000
VERSION     DASWGM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10476
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221703"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGA15_02040"
     CDS             <1..187
                     /locus_tag="VGA15_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586513.1"
                     /translation="ALAAVVKADGHILTSNSYVTIARSDDAGFVARLYTAGALLVLDA
                     EQTGGCGGLPPKRAASL"
     gene            complement(200..1798)
                     /locus_tag="VGA15_02045"
     CDS             complement(200..1798)
                     /locus_tag="VGA15_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9586514.1"
                     /translation="MQKFSKKFKTSASAIALLVVVFGVYGLRKLQVLAADPVGEKDCR
                     PAADGAEQGRSDLDRIKAIAPLADVKWSQLGGSINDASCLNKTEIYGIVEVHSVDDVA
                     KTLAFARDNKLSITTAGVRHSMGGQAFRKGGIVLDMRGFNRIVLNESTRTITVQPGAT
                     WHDIQNALHPRFAVRAMQSTDIFSVGGSISVNAHGMDHQAGAVAKSIKSMRVMLADGS
                     LRTVSPTENRELFDLVVGGYGLFGVIVEAELDIADNLVYQTGRQVLDYRVFPALFAGE
                     IEKDTNIGLMYGHLSTAPSTFLQEMLLYTYTKVDGDDFSRAPLGEVSGTKLRRLTINL
                     SKQGPLFQELKWLSEKHIEHRMETCTVTRAQALPSAEACLVSRNDPMHDSVPYLRNSL
                     PNDTDILHEYFIPRSQFVSFVDGMRKVLTENKTNLLNASVRIVHQEENFLTYSPEPAF
                     SLVLYINQTTDDEGNQRMRKATEELIDLTIAHKGRFFLPYQLYYSRDQLQRSYPQINE
                     FFAAKRRYDPGEVFTNTFYQKYAT"
     gene            2006..2719
                     /locus_tag="VGA15_02050"
     CDS             2006..2719
                     /locus_tag="VGA15_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX9586515.1"
                     /translation="MLQACLNGDRDKSFHAATPLMPRELAADARAVVVAGAEQLHIHP
                     RDAEGRESLHPDDTAAALEAIRASVPGIPVGVSTGWWIAPGGRARQQHIRAWQVKPDY
                     VSVNLIEGDAGEVIDLVLENGIGVEAGLWSAADAGKFVAHPNARSCLRVLIEINEQDF
                     SAGAEAARQIVEILDHAEIQLPRLLHGFESTMWPFYREALRLKLDARIGLEDGSLLPS
                     GTEAENNAALIRAACALTG"
     gene            2781..3749
                     /locus_tag="VGA15_02055"
     CDS             2781..3749
                     /locus_tag="VGA15_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9586516.1"
                     /translation="MLTRRGILLASIAAGITTMPNRSVFATAAQPSTPVNFDIPAGAC
                     DCHTHIHGDPAKFPFFAGRVYTPELASPEEMSALHKALHIERVVIVTPSIYGPDNSAT
                     LFGMKARGATARGVAVIDDKTAESDLDAMNQAGIRGIRLNLATGGVNDPAVGRPRFQA
                     AVDRVKARGWHIQLFTNLAMISAIKDLVATSPMPVVFDHFGGAQAELGVDQPGFADLV
                     ELVRTGKAYVKISGAYRASKLGPDYADAAPLARALIAANFDRIVWGTDWPHPDSVTPP
                     GRKVSDVTPLIQIDDGRLLNQLPVWAPDAAIRQKILVDNPAKLYGF"
     gene            complement(3737..4648)
                     /locus_tag="VGA15_02060"
     CDS             complement(3737..4648)
                     /locus_tag="VGA15_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586517.1"
                     /translation="MTATDQASSGKLAGLLSSASVEVSSRGHQLQELRDNFARGTDVT
                     ITFLPGDNYRRNVETASALRRAGYNPVPHIAAREMPSREALDDFLARARGEADVTRVL
                     LIAGDLALAKGPFRSSLDICASGLIEARGILSVSVAGHPEGHPYLSAANAFKVLEAWR
                     DWGQLTKIRVDVVTQFCFESPPILGWIGELDARCIGLPVIVGLAGPATPATLTKFALR
                     CGIGNSMRSLRSQIGRFGRLLIDTGPDDVMRGLQSASKAATAPIAGFHLFPFGGLRKA
                     SEWLRSYRQETLRQMEQATSGTASQNP"
     gene            complement(4717..6129)
                     /locus_tag="VGA15_02065"
     CDS             complement(4717..6129)
                     /locus_tag="VGA15_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586518.1"
                     /translation="MRVEERQRQTTATADQILGEIRDYCRATQTAESTFGRRVVNDGK
                     LVSRLRDGARITTGTLDKVRAYLSEYRGASPSAPSTLSPPAGKPLNGAAAADVAPAGF
                     RFFDNRQKYLLFVSTCSEKTEVTHRISLELGNLQPTPPALRIFDAGVGDGTVLSRVMR
                     ALHARFPTMPLYAVAKEISYEDVRLMLDKMADRLFEHPATVLVITNLYYAEAPWLTPR
                     SATSAQSLIWKDVALSGNTAHGFAEQIGELEGFLAENWRAGISPTTGNPVYTRPVILT
                     LRREDHSFLLDPIMPRPGRVMADYDLVIASQPYRARASVEFKASKVVAPLVRSLRPGG
                     RLIGIHSHGDDPGMEIIDGVWPGDNPFTSDRHAILRQVKHELGAAARHYNFNASSDAR
                     SIFRYRMHTLPDEVSGPIGTSTLFAAWNAAIYVAQIEDIRLSEVVRDGRYLEATDRVL
                     KQHGGLWFNDETYVISRRRA"
     gene            complement(6305..9427)
                     /locus_tag="VGA15_02070"
     CDS             complement(6305..9427)
                     /locus_tag="VGA15_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX9586519.1"
                     /translation="MDRLVALAVSRRYLMVGLFAVVIIGGLIAFKQLNIEAYPDPTPP
                     MVDIVTQSPGLSSEEIERYITIPIETQVAGIKNLRTIRTISLYGLSDVKLQFSFDYTY
                     DEALQQVLNRLSQLAPLPGNVQPGISPLSAIGEIYRYRLAGPPNYSVLDLKTLQDWVL
                     QRRFRAVPGVIDVTGWGGKTKTYELQVDFNKLIAYGLTLPQLLQAVSNSNINVGGNTV
                     NIGAQSAVVRGVGLIRSIDDLASTMVSQSGGNPVLVKDIATVTVGEKPRLGIAGLDQD
                     DDIVQGIVLMRRGEQSSPTILRVEELVNSINNSSILPPGVRIERIYDRKDLIDITTAT
                     VLHNMVLGMLLIVLLQWIFLGDLRSALIVGATIPFALFFAVIILVLRGESANLLSVGA
                     IDFGLIVDATVIMVEAIFRRLSQTTALSEAEQSHISFDTTMGMKSHAILSAAADVSRS
                     IFFAAAIIIAAFLPLFTLSGVEGNIFGPMARTYAYALAGGLLATFTVTPALSAIILPS
                     HMHETETRIMAFLHRLYVPVLNWAVANRNLVMAAAAGLVVMTIVFGRLLGLEFLPKLE
                     EGNLWIRATLPPTISLQEGNAYVNEMRKMIRSRPEVEAVVSQHGRPDDGTDAAGLFNA
                     EFFAPLKPVNEWPGTHDKDELTAQLLGQLQDKFPGVEFNFSQYLQDNVSEAVSGVKGE
                     NSIKLYGNDLQALSDTANKIKSVLSTVQGITDLAVFTSLGQPTIQIDIDRAKAARYGL
                     APGDINATIKVAIGGDSAGDLYEPGSDRHFPIIVRLAPEYRKSAEAIQNLRIGAAGPN
                     GTITQIPLSEVASINLVSGAAYIYREQQERYLPIKFSVRERDLGGAVREAQDKVAAQV
                     QLPPGSRLEWVGEFGNLQDAIKRLSIVVPISLALIAVLLWFNFGSMIDTLLAMSVIPM
                     AIFGGVLGLLISGIPFSVSAAIGFIALFGIAVMDGIIILSQYNQLIDEGFDRARAVIR
                     TGELQLRPVLMTCVVAGVGLLPAALSAGIGSQVQKPLAVVVVTGMMLAPLVILVTLPV
                     LISYFSRRAR"
     gene            complement(9454..>10476)
                     /locus_tag="VGA15_02075"
     CDS             complement(9454..>10476)
                     /locus_tag="VGA15_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9586520.1"
                     /translation="ERTFRAETVTEGKVAVDEDRSTPVFSPYAGRVTKLMVRPGDSVT
                     QGQPLFVIEAADTVQAQNDFITAMTGLNKAKSALDLARIQDTRAKDLFEGKAVPLKDY
                     QQTQAALIQAQNDLRSSQTALEAARNKLRILGLTDEAISTFQEKGRIDPNTTIFSPIK
                     GTVVQRKVGPGQYVNSGASDPVFVIGDLSTVWLTAFVRETDASRVSVGQEIGINVLAL
                     PGRPLTARINYVSAAIDPATRRLLVRATVDNKDGLLKPEMFANVTIYSAGDHPAIGVP
                     KQALIYEGGQVRVWVAHEDKSIELRQIKPGLTNGDLVEVEGNLKPGEQIVTKGSLFID
                     RAASGS"
BASE COUNT         2020 a         3261 c         3238 g         1957 t
ORIGIN      
        1 agccctggcg gccgtcgtca aggcggacgg ccacatcctc acgtcaaaca gttatgtcac
       61 gatcgcgcgt tccgatgacg ccggtttcgt ggcccggctc tacacggcgg gcgcgctgct
      121 ggtgctggat gccgaacaaa ccggcggctg cggcggcttg ccgcctaaac gtgccgcaag
      181 cctctgatgg gtggcggttc tacgtggcgt atttttgata aaacgtgttg gtgaagactt
      241 caccgggatc gtatctgcgt ttcgccgcga agaattcgtt gatttgcggg taggagcgct
      301 gcagctgatc gcgcgaataa tagagctggt aaggcaggaa gaaccggccc ttgtgggcga
      361 tcgtgagatc gatcagctct tccgtcgcct tcctcatgcg ctggttgcct tcgtcgtcag
      421 tggtctggtt gatatagagc accagcgaaa acgccggctc cggcgaatag gtcaggaaat
      481 tttcctcctg atgcacgatc cgcaccgacg cgttgagcag attggtcttg ttctcggtca
      541 gcaccttgcg catgccatcg acaaaggaga cgaactgact gcgcggaatg aaatattcgt
      601 gcaggatgtc ggtgtcgtta ggtagcgagt tgcgcaagta aggcacggaa tcgtgcatcg
      661 gatcgttgcg gctaacgagg cacgcctcgg ccgacggaag cgcctgcgcg cgcgtcaccg
      721 tgcaggtttc catccggtgc tcgatgtgct tctcggacag ccatttcaat tcctgaaaca
      781 gcgggccctg cttcgacaga ttgatggtca gccgccgcag ttttgttccg ctgacctcgc
      841 cgagcggtgc gcgtgagaaa tcgtcgccgt ccaccttggt gtaggtatag agcagcattt
      901 cctgcagaaa ggtgctcggc gcggtcgaga ggtggccgta catcaggccg atattggtat
      961 ctttctcgat ctcgcccgcg aacagcgccg gaaataccct gtagtcgagc acctgccgcc
     1021 cggtctggta gaccaggtta tccgcgatat cgagctcggc ctcgacgatc acgccgaaca
     1081 ggccgtagcc accgaccacc aggtcgaaca attccctgtt ttcggtcggc gaaaccgtgc
     1141 gcagcgaacc atccgccagc atgacgcgca tcgatttgat cgacttggca accgcaccgg
     1201 cctgatgatc catgccgtgg gcgttgaccg aaatcgagcc gccaacgctg aaaatatcgg
     1261 tcgattgcat ggcgcgaacc gcgaagcgcg gatgcagcgc gttctggata tcgtgccagg
     1321 tcgcccccgg ctgcacggtg atcgttcttg tgctctcgtt gaggacgatc cggttgaagc
     1381 cccgcatgtc aagcacgatg ccgcccttgc gaaaagcctg ccctcccatg ctgtggcgta
     1441 cgccggccgt cgtaatcgag agcttgttgt cgcgggcaaa cgccagcgtc ttcgcgacgt
     1501 catcaacgct gtgcacttcg acgatgccgt atatttcagt cttgttgagg cagcttgcat
     1561 cattgatgct gccgccgagc tgggaccatt tcacatcggc aagcggcgca atcgccttga
     1621 tccgatcgag atcgcttctg ccttgctcgg cgccgtcagc ggcgggccgg caatcctttt
     1681 cgccgacggg atcggcggct agaacctgaa gcttgcgcaa gccgtaaacg ccaaacacca
     1741 cgaccagcaa ggcgatcgcc gacgcgctgg ttttgaattt cttcgaaaat ttttgcatca
     1801 attgattcca gacgaggtct gataatctag ccgcgcggag gactagtcaa atccagaact
     1861 ttactgctcg ggaattgccc tgccgacgcc gtccgggcag tgaaatccgc tgaaaacgat
     1921 tcgcttcggc gggagcaagt ctcgcttgac cggccctacg gggcttgtga cattgcatct
     1981 tgatcgtgtc cccgagactc tcccgatgct tcaagcctgc ctgaacggcg accgcgacaa
     2041 aagcttccac gccgcaaccc cgctgatgcc gcgggaactg gcagccgacg ccagggctgt
     2101 cgtggtggcc ggcgcagagc aattgcacat ccatccgcgc gatgccgagg ggcgggaaag
     2161 cctgcatccc gacgacaccg ccgcggcact cgaggccatc agggccagcg tgcccggaat
     2221 tcccgtgggc gtatcgaccg gctggtggat tgctccggga ggacgggcgc ggcaacaaca
     2281 tatcagggcg tggcaggtaa agcccgatta tgtctccgtc aatctgatcg agggagacgc
     2341 cggcgaagtc atcgatcttg tcctcgaaaa cggcatcggc gtcgaggcgg gcctgtggtc
     2401 tgccgccgac gccggaaaat tcgtggcgca tcccaacgcc cgcagctgcc ttcgggtact
     2461 gatcgaaatc aacgagcagg atttttcagc tggcgcagag gctgcgcgtc agatcgtgga
     2521 aatcctggac cacgccgaga ttcaactacc gcgtttgctg catggattcg aaagcacaat
     2581 gtggccgttc tatcgcgaag cgcttcgcct caaactcgat gcgcggatcg gactggaaga
     2641 tggcagcctg ctgccgtccg gaaccgaggc tgaaaataat gccgccttga tccgcgcggc
     2701 gtgtgcgctg accggataac ctgctacttt gcacccaagc gcagaactaa aagcgcggaa
     2761 aacgcaacag ggagacgacc atgctgacgc gacgcggtat cttgcttgcc tccatcgcag
     2821 ccggaataac aaccatgccc aaccgatccg tttttgcaac cgcggcgcag ccctcgacgc
     2881 cggtgaattt cgacattccc gccggcgcct gcgactgcca cacccatatc catggcgacc
     2941 ccgccaaatt cccgttcttc gcgggccgcg tctatacccc cgaactggcg tcgcccgaag
     3001 agatgtcggc cctgcacaag gcgctgcata tcgagcgcgt agtgatcgtc acgccgagca
     3061 tctacggccc ggataattcg gcaaccctgt tcggcatgaa ggcgcgcggc gcgaccgcgc
     3121 gcggcgtcgc cgtgatcgac gacaagacag cggagagcga tctcgacgcc atgaaccagg
     3181 cgggtatccg cggcatccgg ctcaatctcg cgaccggcgg cgtcaacgat cccgcggtgg
     3241 gccgtccgcg ctttcaggcc gcggtcgacc gcgtcaaggc tcgaggctgg catatccagc
     3301 tgttcacaaa tctcgcgatg atctcggcga tcaaggatct cgtcgcgacc tccccaatgc
     3361 ccgtcgtgtt cgaccacttc ggcggcgcac aggccgaact cggcgtcgac cagccgggct
     3421 ttgcagatct cgtggagctg gtgcggaccg gcaaggcgta tgtgaaaatc tccggcgcct
     3481 accgggcctc aaagctcggt cccgattatg ccgacgccgc cccgctcgcc cgcgcgctga
     3541 tcgcggcaaa cttcgaccgc attgtctggg gcacggattg gccgcatccg gattcggtga
     3601 cgccgccggg acgaaaagtc agcgacgtga cgccgctgat tcagatcgac gacggccggc
     3661 tgctcaatca gctcccggtg tgggcgccgg atgcggcgat ccgccaaaag attctggtcg
     3721 acaatccggc gaagctttac ggattctgag aggcggttcc cgaggtcgcc tgctccattt
     3781 gtcggagcgt ttcctggcgg tagctgcgca accattcact ggctttgcgc aagcccccga
     3841 agggaaacaa atggaatccg gcaatcggcg cggtcgcagc tttggacgcc gattgcagac
     3901 cgcgcatcac atcatccggc ccggtatcga tcaaaagccg gccgaaacgg ccgatctggc
     3961 tgcgcagcga ccgcatcgaa ttgccgatgc cgcagcggag cgcgaatttc gtcagcgtcg
     4021 ccggcgtcgc cggaccggcg aggccgacga tcaccggaag gccgatacag cgcgcatcga
     4081 gttcgccgat ccagccgaga atcggtggac tttcaaaaca aaattgcgtc acgacatcga
     4141 cgcggatttt tgtcagttgc ccccagtccc gccacgcttc aagcaccttg aaggcattcg
     4201 ctgccgagag ataagggtgg ccttcgggat gaccggcaac gctgacggac aaaatgccgc
     4261 gcgcctcgat cagtccgctg gcgcaaatat cgaggcttga cctgaacgga ccctttgcca
     4321 gcgccaaatc gccggcgatc agaaggacgc gcgtgacatc cgcctcgccg cgcgcccgcg
     4381 ccagaaagtc gtcgagcgcc tcgcgcgacg gcatttcgcg cgcggcgata tgcggaacgg
     4441 gattgtaacc ggcgcggcgc agcgccgatg ccgtctcgac gttacggcga taattgtcgc
     4501 cgggcaggaa ggtgatcgtc acatccgtgc cccgagcaaa attatcccgc aactcctgca
     4561 actgatgccc acgcgaggag acctcgacgg aggccgatga cagcaggccg gcgagttttc
     4621 cggacgaagc ctgatcggtt gcggtcacat ttcactccga ggatggttgg catgatcttt
     4681 ttccgaaaac cggttcccac ttttcgggat catgccttaa gcccggcggc gcgaaatcac
     4741 ataggtttca tcgttgaacc agagaccgcc atgctgcttc agcaccctgt cggtggcttc
     4801 gaggtagcga ccatcgcgga ccacctcgga caacctgatg tcctcgattt gcgcgacata
     4861 gatcgcggca ttccaggcgg cgaacagcgt cgaggttccg atcgggccgc tcacttcatc
     4921 ggggagcgtg tgcatgcgat agcggaagat cgaacgggcg tcggacgatg cgttgaaatt
     4981 gtagtgccgc gccgcagcac caagttcgtg cttgacctgt cgcaggatgg cgtgacgatc
     5041 cgacgtgaac ggattgtcgc caggccagac tccgtcgatg atctccatcc ccggatcatc
     5101 cccatgcgaa tggatgccga tcagccggcc gccgggccgc agcgaccgca ccagcggcgc
     5161 cacgaccttc gatgccttga attcgacgct cgcccgcgcc cgatagggct gcgaggcgat
     5221 gacgagatcg tagtcggcca tcacgcgccc cggccgcggc atgatcggat cgagcaggaa
     5281 cgagtggtcc tcccgccgca aggtcaggat gacgggcctt gtatagaccg ggttgccggt
     5341 ggtcggactg atcccggcac gccagttctc ggccaaaaac ccttcgagtt cgccgatctg
     5401 ctcggcaaag ccgtgagccg tattgccgga cagcgcgaca tccttccaga tcaggctttg
     5461 cgctgatgtc gccgagcgcg gtgtcagcca tggcgcctcg gcataataga gattggtgat
     5521 gacgagcacc gtggcgggat gttcgaacag gcggtccgcc atcttatcga gcatcaaccg
     5581 cacatcttcg tagctgattt ccttggcgac ggcataaagc ggcatggtcg ggaagcgcgc
     5641 gtgcagcgcc cgcatcaccc gcgacagcac ggtgccgtcg ccgacaccgg catcgaagat
     5701 ccgaagcgcc ggcggggtcg gctgcagatt gccgagttcg agagaaatcc ggtgcgtgac
     5761 ctcggtcttc tcgctgcagg tcgagacgaa cagcagatat ttctgccggt tgtcgaaaaa
     5821 ccggaagccg gcgggcgcaa cgtcggccgc ggcggcgccg ttcagcggct ttccggccgg
     5881 cggcgacagc gtgcttggcg ccgacggtga cgcgccacga tactcggaca gataggcgcg
     5941 gaccttgtcg agggtgccgg tggtgatcct tgcgccgtcg cgcaagcgcg acaccagctt
     6001 gccatcgttg acgacgcgcc gcccaaacgt ggattcggcg gtttgggtcg cgcggcagta
     6061 atcccggatc tcgccgagaa tctgatcggc ggtggcggtg gtctgccgct gacgttcctc
     6121 gacccgcatg gcgaactccc cgcggttttg atccctgacg caatcaagtg acggacgtca
     6181 ggttacgtca accacaatcg gagtgggcaa cggatatggg catgtgccca cctatttcac
     6241 gcgaaccggt ctccaccccg gatcaagtcc ggggcaggct ttcaccggaa aacgctctgg
     6301 ccgcttaccg tgcccgacgc gagaaatacg agatcagcac cggcagcgtc accagaatga
     6361 ccagcggcgc cagcatcatg ccggtgacca ccaccaccgc cagcggcttt tgcacctgcg
     6421 atccgatgcc cgccgacagc gccgccggca acagcccgac cccggcgacg acgcaggtca
     6481 tcagcaccgg ccgcagttga agctcgccgg tgcggatcac cgccctcgcc cggtcaaagc
     6541 cttcgtcgat caattgattg tattgcgaca ggataataat gccatccatc acggcaatgc
     6601 cgaacagcgc gatgaacccg atcgccgcgg agacgctgaa cggaatgccc gagatcagca
     6661 gcccgagcac gccgccgaag atcgccatcg gaatcacgct catggcgagc agcgtgtcga
     6721 tcatcgaacc gaaattgaac cacaacagca ccgcgatcag cgcgaggctg atcggcacca
     6781 cgatcgacag ccgcttgatg gcgtcctgca gattgccgaa ttcgccgacc cattcgagcc
     6841 gcgatcccgg cggcagctgg acctgggccg cgaccttgtc ctgcgcctcc ctgaccgcgc
     6901 cgccgaggtc gcgttcgcgc accgagaatt tgatcggcag atagcgttcc tgctgttcgc
     6961 ggtagatgta ggccgcaccc gagaccaggt tgatggatgc aacttcgctc aaggggatct
     7021 gcgtgatggt gccgttcggg cccgccgcac cgatccgcaa attctggatc gcctcggcgc
     7081 tcttgcgata ttcgggggcg aggcggacga tgatcggaaa atgccggtcg ctgccgggtt
     7141 catagagatc gccggcgctg tcgccgccga tcgccacctt gatggtggca ttgatatctc
     7201 ccggcgcgag cccgtaacgg gccgccttgg cgcgatcgat atcgatctgg atggtgggct
     7261 ggccaagcga ggtgaacacc gccagatcgg taatcccctg cacggtggac agcacggact
     7321 tgatcttgtt ggcggtgtcg ctgagcgcct gcagatcgtt gccatacaac ttgatcgagt
     7381 tttcgccctt cacgccggag acggcttcgg aaacgttgtc ctgcaagtat tgcgagaaat
     7441 tgaactcgac gccgggaaac ttgtcctgca gctgccccag caactgcgcg gtgagttcgt
     7501 ccttgtcatg cgtgcccggc cattcgttga ccggttttaa cggcgcgaag aactcggcgt
     7561 tgaacaggcc cgcggcgtcg gtgccgtcgt cggggcggcc atgctgcgac accaccgctt
     7621 ccacctcggg gcggctgcgg atcatcttgc gcatctcgtt gacataggcg ttgccttcct
     7681 ggagcgaaat agtcggcggc aaggtcgcgc gaatccagag attgccctcc tcgagcttgg
     7741 gcaggaactc cagtcccaac aggcggccga agacgatggt catcaccaca agccccgcgg
     7801 ccgccgccat caccagattt cgattggcga cggcccagtt caacacggga acgtagagcc
     7861 gatgcaggaa cgccatgatc cgggtttcgg tttcgtgcat gtgcgagggc aggatgatcg
     7921 cactcaacgc aggggtcacg gtgaaggtcg cgagcaaccc gcccgccagc gcataggcat
     7981 aggtccgcgc catcggcccg aagatgttgc cttcgacgcc gctcagcgtg aacagcggca
     8041 ggaacgcggc gatgatgatg gcggcggcaa agaaaatcga ccgcgacacg tcggcggcgg
     8101 ccgagagaat ggcatggctc ttcatgccca tggtggtgtc gaatgagata tggctctgct
     8161 cggcttccga aagcgctgtg gtctgcgaca gccggcggaa gatcgcttcc accatgatca
     8221 cggtggcgtc cacgatcagc ccgaaatcga tcgcgccgac cgacagcagg ttggcggatt
     8281 cgccgcgcaa caccagaatg atcacggcga aaaacagcgc gaacggaatg gtggcgccga
     8341 cgatcagggc gctgcgcaga tcgcccagaa aaatccattg caacagcacg atcagcagca
     8401 ttcccagcac catattgtgc agcacggtcg cggtggtgat gtcgatcaga tccttgcggt
     8461 cgtagattcg ctcgatccgc acgcccggcg gcaggatgct ggagttgttg atcgagttga
     8521 cgagctcctc gacgcgcaga atcgtcggcg aactttgctc gccacggcgc atcagcacga
     8581 tgccctgcac gatgtcgtcg tcctgatcga ggccggcgat gccgagccgc ggcttctcgc
     8641 cgacggtcac ggtagcgata tccttgacca gcaccggatt gccgccgctt tgcgacacca
     8701 tggtgctggc aagatcgtcg atggaacgga tcaggccgac gccgcgcacg acggcggatt
     8761 gcgcgccgat gttgacggta ttgccgccga cattgatgtt ggagttgctg acggcctgca
     8821 gcagttgcgg cagcgtcagg ccgtaggcga tcagcttgtt gaaatcgacc tgcaattcat
     8881 aggtcttggt cttgccaccc cacccggtga cgtcgatcac gccgggcacg gcgcgaaagc
     8941 ggcgctgcag aacccagtcc tgcagcgttt tcaggtcgag cacgctgtaa ttcggcggac
     9001 cggccaagcg gtagcggtag atttcgccga tcgcgctgag cggcgaaatt cccggctgca
     9061 cgttgccggg caacggcgcc agttgcgaca gccggttcag cacctgctgc agcgcttcgt
     9121 cataggtgta gtcgaacgaa aactgcagtt tgacgtccga caatccgtac agcgagatgg
     9181 tgcggatcgt gcgcaggttc ttgatgcccg cgacctgggt ctcgatcgga atggtgatgt
     9241 agcgttcgat ctcttccgac gacaaccccg gactttgcgt cacgatgtcg accatcggcg
     9301 gcgtcgggtc ggggtaggct tcgatattga gctgcttgaa tgcgatcagg ccgccgatta
     9361 tcacgacggc gaacaggcct accatcaaat agcggcggct gactgcaagg gcgacgaggc
     9421 gatccattca gatcgggctt tcaatgggct gagttagctg ccggatgcgg cgcgatcgat
     9481 gaacaggctg cctttcgtca caatctgctc gccgggtttc aaattgccct cgacctcgac
     9541 gagatcgcca ttggtgaggc cgggtttgat ctggcgcagt tcgatcgact tgtcctcgtg
     9601 cgcgacccac acccgcacct gaccgccctc gtagatcagc gcctgttttg gcacgccgat
     9661 cgccgggtga tcgccggcag aatagatcgt gacattcgcg aacatttccg gcttcaacag
     9721 accgtccttg ttatccacgg tggcgcggac cagaagccgg cgggtcgctg gatcgatcgc
     9781 cgcggaaaca tagttgatac gcgccgtcaa cgggcggccc ggcagcgcca gcacattgat
     9841 gccgatctcc tgccccaccg aaaccctcga cgcatcggtt tcgcggacga aggccgtgag
     9901 ccagaccgta gagaggtcgc cgatcacgaa taccggatcg ctggcgccgg agttcacata
     9961 ttggccgggg ccgacctttc gctgcacgac cgtgcccttg atcggcgaga agatggtggt
    10021 attcggatcg atgcggccct tttcctgaaa ggtcgatatc gcctcgtcgg tcaggccgag
    10081 aattcgcaat ttgttgcgcg ccgcctccag cgccgtttgt gacgaacgca agtcgttctg
    10141 cgcctggatc agggcggcct gggtctgctg ataatccttg agcggaacgg ccttgccctc
    10201 gaacagatcc ttggcgcgcg tgtcctgtat ccgcgcgaga tcgagtgccg attttgcctt
    10261 gttcaagccg gtcatggccg tgatgaaatc gttttgcgcc tgcacggtgt cggccgcttc
    10321 aatcacgaac agcggctgcc cttgcgtgac gctgtcgccc ggccggacca tcagtttcgt
    10381 gacacggccg gcatagggcg aaaacaccgg cgtcgagcga tcctcatcga ccgcgacctt
    10441 gccttcggtc acggtctcgg cccggaacgt gcgctc
//
LOCUS       DASWGM010000063         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2499359,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000063 DASWGM010000000
VERSION     DASWGM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499359"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VGA15_02080"
     CDS             complement(<1..1310)
                     /locus_tag="VGA15_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX9586521.1"
                     /translation="MLGLMQDWPLLCHRIIEHAAAVHGTQEVVTRSVEGPIHRTNYAE
                     IRGRALKVSQRLDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTINPRLFPDQ
                     IAWIVNHAEDRLVMTDITFVPILEKIADKLPSVERYIVLTDGAHMPKTTLKNAVAYED
                     WIAEADGDFKWKTFDENTAAAMCYTSGTTGDPKGVLYSHRSNVLHALMANTGDSLGAN
                     AADTMLPVVPLFHANSWGIAFSAPSMGTKLVLPGAKLDGASVFELLDTEKVTHTAGVP
                     TVWLMLLNHIAANHLKLPNLRMVVCGGSAMPRSMIKSFLDMGVQVRHAWGMTEMSPIG
                     TLASLKPPFSELTGEARLDILQTQGYPPFGVQMKITDDAGRTLPWDGKTFGRLKVSGP
                     SVSKAYFRIDADILDEEGYFDTGDVATIDANGYMRITDRSKDVI"
     gene            1438..>1976
                     /locus_tag="VGA15_02085"
     CDS             1438..>1976
                     /locus_tag="VGA15_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586522.1"
                     /translation="MVTAVTALMLLCVQTEPAVARRHTQNLWGDLFGENRPRLRPRQR
                     RAAALPPSIPLPKPRPAEAPVIEPEKPAAAKPAPAATDKPIEQASSPLPKPRPADAPV
                     TEPEKPAAKPAPAATDKPAEQATAPPPPSACRLALTDAIAIAPSIPDIKGPGECGAED
                     VVRLEAVVLPDKRRVSVKPA"
BASE COUNT          369 a          623 c          622 g          362 t
ORIGIN      
        1 ttgatcacat ccttggaacg atcggtaatc cgcatgtatc cgttggcgtc gatggtcgcg
       61 acgtcgccgg tgtcgaaata gccttcctcg tcgaggatgt cggcatcgat gcggaaatag
      121 gccttcgata ccgagggacc gctgactttc agccggccga acgtcttgcc gtcccatggc
      181 agggtcctgc cggcatcgtc ggtgatcttc atctgcacgc cgaagggcgg atagccctgg
      241 gtctggagga tatcaagccg ggcctcgccg gtgagttcgc tgaacggagg tttcagcgag
      301 gcaagggtgc cgatcgggct catctcggtc atgccccaag cgtggcgcac ctgcacgccc
      361 atgtcgagga acgacttgat catcgagcgc ggcatcgccg agccaccgca gaccaccatc
      421 cgcagatttg gcagtttcag atggttggcg gcaatgtgat tgagcagcat cagccacacc
      481 gtcggaacgc cggcggtatg cgtcaccttt tcggtatcca acagctcgaa caccgacgcg
      541 ccatcgagtt tcgcgccggg cagcaccagt ttggtgccca tcgatggcgc cgagaacgcg
      601 ataccccagc tgttggcatg gaacagcgga accaccggca gcattgtatc cgcagcgttc
      661 gcaccgaggg aatcgccggt gttggccatc aaggcgtgga gcacgttgga acgatgcgaa
      721 tacagcacac ctttgggatc gcccgtcgtg ccggaggtgt agcacatcgc ggccgcggta
      781 ttttcgtcaa acgtcttcca tttgaaatcc ccgtccgcct ctgcgatcca gtcctcatag
      841 gcgaccgcgt tcttcagcgt ggttttcggc atgtgcgcac cgtcggtgag cacgatgtag
      901 cgttccacgc tgggcagctt gtcggcgatt ttttccagga tcggcacaaa ggtaatatcc
      961 gtcatcacca ggcggtcttc cgcgtgattg acgatccagg cgatctggtc ggggaacagc
     1021 cgcggattga tggtgtggca gatcgccccg atgcccatga tgccgtacca ggcttcgaga
     1081 tgtcgccagg tgttccaggc gatggtggcg acacggtcgc cgagcttgat gccgtcgcga
     1141 tcgaggcgtt gcgagacctt cagggcgcgg ccccggattt cggcgtaatt ggtgcgatga
     1201 atggggcctt cgaccgatcg cgttaccact tcctgcgtgc cgtgaaccgc cgctgcgtgc
     1261 tcgataatcc ggtggcaaag caaaggccag tcttgcatca gtccaagcat gcgaacgttc
     1321 ctccagattg tgctaccgct tgtcgaaggt ctttacggaa ttgccatgaa ctctagcgcg
     1381 cagaaagcaa gccgcaaatg gccttatagc ggctttgaca gcgccgcagc ggcgatcatg
     1441 gttaccgctg taaccgcgct aatgctgctt tgcgtgcaga ccgaacctgc tgtggcgcga
     1501 agacacactc aaaatctgtg gggcgatctg ttcggcgaga acaggccaag gctgcggcca
     1561 aggcagcgcc gggccgccgc gctcccccca tcgataccgt tgccgaagcc gcgtccggcc
     1621 gaggccccgg tgatcgagcc ggaaaagccc gcggccgcaa aaccggcgcc cgccgcgact
     1681 gacaagccga tcgaacaggc ctcgtcaccg ttgccgaagc cgcgtccggc cgatgccccg
     1741 gtgaccgagc cggaaaagcc cgccgcaaaa ccggcgcccg ccgcgaccga caagccggcc
     1801 gaacaggcca cggccccgcc gccgccgtcg gcctgccggc tggccctgac cgatgcgatc
     1861 gccattgccc ccagcatccc cgacatcaag ggtcccggcg aatgcggcgc ggaggatgtg
     1921 gtgcggctgg aggccgtggt gctgccggac aagcggcggg tttcggtaaa acccgc
//
LOCUS       DASWGM010000064        15479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1667769,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000064 DASWGM010000000
VERSION     DASWGM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15479
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667769"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VGA15_02090"
     CDS             complement(<1..836)
                     /locus_tag="VGA15_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586523.1"
                     /translation="MERKQRLPPSRERNVESFDEDASVIEFMSYRSAPPMSKGGVRPD
                     DAMPLFLSDPEAEEEEAPRRPSFGRIRDHEDDEPPRVLKSSIAPRIIKVGIFAISAAA
                     IIAAILSVQNPLDLFTNAKASLTSSSGGQPAAIPSATAQLRPAPEPVVAARTASADPA
                     AQEIQPNLGARASSPTAKGGPTRDEIASAFRTARQDQPEVRLPPAAVAPPPTAAPPAA
                     APAAVAALPPAAAFPPPTADLPAAAAPTAKRLAADELAALLKRAKGLIAIGDFAPARL
                     LL"
     gene            complement(1092..2066)
                     /locus_tag="VGA15_02095"
     CDS             complement(1092..2066)
                     /locus_tag="VGA15_02095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX9586524.1"
                     /translation="MKAILCSQYCGPDELVLADVPDPVAEAGQAVIAIKSAALNFFDL
                     LMIQGKYQIKPPFPFSPAAEVAGVIESVGAGVTDLKVGDRVVASCGHNGAREKIALPA
                     NTIVKIPDNLDFDRAAGIIIIYGTALHALEDRASLKPGETMAVLGAAGGTGLAACELG
                     KLMGLKVIACASSDEKLEFAKAHGAELGLNYATEDLKEGLRRLTGGKGVDIIFDPVGG
                     SYAEAALRSIAWEGRFLVIGFAAGEIPKMPLNLALLKGCDIRGVFWGAWARLNAEKNR
                     ANLQKLVKWTAEGKISSHVDRTFPLAQTADALKVLAGRKAMGKVILHP"
     gene            2370..3209
                     /locus_tag="VGA15_02100"
     CDS             2370..3209
                     /locus_tag="VGA15_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9586525.1"
                     /translation="MFEKGLLAGKRILVTGGGSGLGAAMGRRFVELGAELIICGRRLE
                     LLEATAAQMRGDFAGKVTAIRCDIREGAAVDAMMDAIWGDVPLDVLVNNAAATFIAQT
                     EQLSFRAADAILAPTLHGAMYCTLGAGKRWIEAGHSGVVLSILSTSTITGRAFTVPSA
                     MAKSAILAMTKSLAVEWGPKGIRTVAIAPGAFPTAGASGQLRPEGRDEGWASRNPLGR
                     VGEHRELADLASFLISDSAGYINGEMVVQDGGAHLRGSGAEDLLQWSDAQWEKQRSAR
                     SKS"
     gene            3375..3992
                     /locus_tag="VGA15_02105"
     CDS             3375..3992
                     /locus_tag="VGA15_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX9586526.1"
                     /translation="MSVGQMLTTLAASVLVCGAVISAQAQGASSKNATKPTSSKPEAK
                     SEAKSAAAVAGGAEPTLIGQFGTWGAYTAMPNGKKVCFALAKPSSSKTNPPNRPRDPA
                     YAFVSTRPAEKVVNEVSIMIGYALKPGSESTLEVGGASYAMYTQGDGIWIKNAAEEER
                     MVDAMRKAADATVKGVSAKGTETTDTFSLKGLSQALDKLAQDCKR"
     gene            4300..5517
                     /gene="rlmN"
                     /locus_tag="VGA15_02110"
     CDS             4300..5517
                     /gene="rlmN"
                     /locus_tag="VGA15_02110"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9586527.1"
                     /translation="MTSISIEAGSGANPPLEKIALETYVPPAKPSLVGLSRAEIAEQL
                     GAIGVAPAQRKMRVQQLWHWIYVRGAKKFDEMTSISREMRAQLDSHFTVARPEVVAEQ
                     VSNDGTRKWLLRLPSGDRVEKAHEVECVYIPETDRGTLCVSSQVGCTLNCSFCHTGTQ
                     RLVRNLTAGEIVGQVMVARDRLNDWAGRITPTGSRLVTNVVMMGMGEPLYNFDAVRDA
                     LLIVADNEGIGISRRRITLSTSGVVPNIIRTGDEIGVMLAISLHAVRDELRNELVPLN
                     RKYPIAELLQACRDYPGSSNARRITFEYVMLKGVNDSLEDAKLLVKMLKGIPAKINLI
                     PFNPWPGTRYECSDWDQIEKFSEYIFNAGYSSPVRTPRGRDILAACGQLKSETEKLSV
                     RERQALRAMAMTD"
     gene            5700..6212
                     /locus_tag="VGA15_02115"
     CDS             5700..6212
                     /locus_tag="VGA15_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586528.1"
                     /translation="MALIGRLFAILFGFLAACLVAGMIVVAAVLFPEFSDFASGPIDP
                     SALNIVLGFGFVFVSGFALLPAMIVVAITEAFYIRGVLTYAVGGGVVGLACYLGLIPF
                     DPATLSFDGIVRRHLEIMTGAGIVAGLVYWMIAGRNAGAWREPPRPLPPLPPLPSHSP
                     SPPQHSPPSS"
     gene            6401..7177
                     /locus_tag="VGA15_02120"
     CDS             6401..7177
                     /locus_tag="VGA15_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9586529.1"
                     /translation="MNRSGLLTALALALVIGLLFGIYPELDLKLAALFYDGATQSFPL
                     KLNAMAALARDGAMWIAWGIALPAIVALAVKLVRPDRPLLMSGRAIIFLLATLTLSAG
                     ILTNLTFKSYWGRPRPVVVTQFSGDLPFVPWWDPRGGCSRNCSFFSGEGATAFWTLAP
                     AALTPPAWRPLAYVAATLFGVATSALRMAFGGHFFTDVAAAGLVTFLVVWLAYGYIYR
                     WPSTRLSDESIDAALSRLAWPGYRLLQKWRGRDVGPASSV"
     gene            7262..8491
                     /locus_tag="VGA15_02125"
     CDS             7262..8491
                     /locus_tag="VGA15_02125"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX9586530.1"
                     /translation="MSKDVKKVVLAYSGGLDTSIILKWLQTTYGCEVVTFTADLGQGE
                     ELEPARQKALLLGIKPENIFIEDVREEFVRDYVFPMFRANAVYEGQYLLGTSIARPLI
                     AKKQIELAEKLGADAVSHGATGKGNDQVRYELAYFALKPDITIIAPWREWDLRSREQL
                     IAFAEAHQIPIAKDKRGEAPFSVDANLLHTSSEGKVLEDPALEVPDYVYSRTIDPEKA
                     PDRATTITIDFEKGDATAIDGKKLSPATLLTKLNELGRANGIGRLDLVENRFVGMKSR
                     GMYETPGGTILLVAHRGIESITLDRGAAHLKDELMPKYAELVYNGFWFAPEREMLQAA
                     IDHSQQYVTGRVRLKLYKGNVIMVGRESEYSLYDQDLVTFEEGAVAYDHRDAAGFIKL
                     NALRLRTLGQRKKKLGL"
     gene            complement(8726..10648)
                     /locus_tag="VGA15_02130"
     CDS             complement(8726..10648)
                     /locus_tag="VGA15_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9586531.1"
                     /translation="MTNLRLVLLATSALTATQITALPSHAQTAPQVMAQAGGEVGPDG
                     KPKAPPKGAPAPAPAHPAPPAAAPPPHPAPPAAAPPPPPRPAPPPPAAAPPPRPAPPP
                     PPAAAPPPRPTPPPPAAPPPPPPAPPPHVPPPAAAPPPHPAPPTPPAAAPPPAPPKPP
                     APPSAAPPAPPSAAPAPQLQQRNAPEQLQQKNAPAPVLPPPAGAKQPPAAGAPPAAAP
                     PTQTAPPPAGAKQPPAAGALPAAAPPTQTAPPPAGAGTTPPPPPRNGPPPNAAPNAAP
                     APGGAGGQTSLPPGAPAGAAPLVRHAPPTVSAPIPAAPPPAQALAPIAPGAQAPGPRR
                     MDDFRGARRETQEGGRTVFNEPGRVIVVDPGGQSFIRHNEEDRFRYGARDIRTEQVGG
                     ETRTIVIRPDGSQIITVNGRDGQMLRRIRRDERGREIIIIDNSYRDPRAVGGFYVDLP
                     PPVIRIPYDRYIVDAEDAPPDLIYETMEAPPVDRIERRYSLDEIRYSPSVRQLMPSID
                     LNSINFETGSWEIPPDQVAKLQSIADGLNRAISRNPREVFLIEGHTDAVGSDVDNLSL
                     SDRRAESAAQLLTQQFQVPAENLTSQGYGKQYLKEQTDGPSRINRRVTIRRITPLLNG
                     GTASLPPPPPGIAPPR"
     gene            complement(10850..11143)
                     /locus_tag="VGA15_02135"
     CDS             complement(10850..11143)
                     /locus_tag="VGA15_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586532.1"
                     /translation="MPIEPQEDPPIKPGQPSEPPQESPPGSPRPEVPPPMQEPGEPPR
                     PEELPGNMPDELPVRGPGGPGPPSPAADAGIGDLSGEGPDLDPGAADMPQGTM"
     gene            complement(11343..11729)
                     /locus_tag="VGA15_02140"
     CDS             complement(11343..11729)
                     /locus_tag="VGA15_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9586533.1"
                     /translation="MIDHIGFPVSDYARAKAFYTKALAPLGYSLIMEVTQEQTGHDPA
                     AGFGANGKPDFWIGGEGGLNKPLHVAIVAKDRATVAAFYQTAMAAGGRDNGAPGIRAH
                     YHPNYYGAFVLDPDGHNIEAVCHAPE"
     gene            complement(11764..12618)
                     /locus_tag="VGA15_02145"
     CDS             complement(11764..12618)
                     /locus_tag="VGA15_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9586534.1"
                     /translation="MTIPTLPPGLAAANDPAPPPFGVFAPTSAQAAVIALAQRSRLKR
                     GAFRPMMSRLVNLLRAGPVDVQYQGAAFRFYHQASATERGALFNPDYNVEELDFLRAH
                     TPVGGVFVDVGANVGTYAMVLARHVGASGKVIAIEPHPITHARLAFNRAASGTTQATL
                     VAAAAGPSDGELMIETDGDNLGASHIVSGDHSGKAIKVPSLRLQRILGDAGIGHVDAL
                     KIDVEGFEDRVLTGFFKEAPPSLWPRAVVIEHLSRNEWLDDCIADMVARGYAATGKTR
                     SNTLLVRG"
     gene            complement(12861..13229)
                     /locus_tag="VGA15_02150"
     CDS             complement(12861..13229)
                     /locus_tag="VGA15_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586535.1"
                     /translation="MSHAEDLARRYVHIWNETDRAARRSQVAGLWIENGTHYVRAREV
                     HGHADLELRVATSHQTNVAERNYTFELAGGVEQLRDMVKFTWRMVPNSGGAIAATGLI
                     VLLLAEDGRVRIDYQFIDPD"
     gene            13384..14328
                     /locus_tag="VGA15_02155"
     CDS             13384..14328
                     /locus_tag="VGA15_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9586536.1"
                     /translation="MDPLDDAFSAMRVRESLYARVDAGAPWAIRFRKGKAARFGLVVA
                     GSCWLTTEAPAQSIPLASGDCYVILDGSTYTLGDDPRSPALNCFDVVPRLVDGAVSLG
                     GGGAAATVVTGWFVYDELGARALMALLPRVLHTRVDGYRTDILKATLELLAKETERPG
                     VGSGVVISGLADILFVQAIRSHLNNTSENDVGWLAALSDRRIGLAMRALHGKPADAWT
                     VAKLASLANMSRSAFAARFKARLGEAPLEYLTRWRMFRAGVLLRSTQRSLAEIANEVG
                     YESDAALSKAFHRVVGMTPGAFRKQNAPAPENPRRAVA"
     gene            complement(14338..15270)
                     /locus_tag="VGA15_02160"
     CDS             complement(14338..15270)
                     /locus_tag="VGA15_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9586537.1"
                     /translation="MMTTAHPIILHHFEKSPFSEKVRVVFGLKNIEWISVLISRIMPR
                     PDLMPLTGGYRRTPVMQIGADIYCDTQCIIRELERRFPEPTLFPNGYQGIGWGTAMWT
                     DRSFFQNTVNLVFGSLADQVPQDFIADREKLRGAKFDVPAMTAAIPQMRDQFRAHLQW
                     VETQLGDGRAWMSGDRAGLCDVNAYMNVWYVRAHLPNADEMLAEFGNTRAWEMRMRNV
                     GHGRFREISTSAALDVAASATPQTAILADPNDPNGRKPGDRVEVMPDDYGKIKVSGEI
                     VALSSQRIAVRRHDPRAGEVIVHFPRAGFHVLPA"
     gene            complement(15327..>15479)
                     /locus_tag="VGA15_02165"
     CDS             complement(15327..>15479)
                     /locus_tag="VGA15_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX9586538.1"
                     /translation="ATGERLDNLEDPFRLYRCHTIMNCAKACPKSLNPSEAIAELKLK
                     MVERQI"
BASE COUNT         2705 a         4717 c         5121 g         2936 t
ORIGIN      
        1 ttcagcaaca gtcgagcggg cgcgaagtcg cctatcgcga tcaaaccctt ggccctcttc
       61 agcagggccg ccagttcgtc ggcggcgagc cgttttgcgg tcggagccgc cgcggcggga
      121 aggtcggcgg tgggaggcgg aaaagcggcg gcagggggaa gagcagcaac ggcggctgga
      181 gcggccgcgg gaggcgcggc cgtaggagga ggagcgacgg ccgcgggggg cagacggact
      241 tccggttggt cctgacgggc ggttctaaaa gcagaagcga tttcgtcgcg tgtcggcccg
      301 ccctttgcgg tcggtgacga ggcccgagcg ccaaggttcg gttgaatttc ctgcgccgcc
      361 gggtctgcgc tcgccgttcg tgcggcgacg acaggttccg gagcgggtct cagttgtgcg
      421 gtggcggacg gtatggcggc aggttgcccg cccgacgagc tggtgagcga ggctttggcg
      481 tttgtgaaca ggtcaagcgg attctgaacg gataagatcg cagcgatgat tgccgccgcc
      541 gatatcgcaa aaataccgac cttgatgatg cgcggcgcga tcgacgactt gagaactcgc
      601 ggcggctcgt catcctcgtg atcccggatc ctcccgaaac tcggccgtct gggtgcctct
      661 tcttcttctg cttcaggatc ggagaggaag agcggcatgg cgtcgtccgg acggacaccc
      721 cccttggaca ttggtggcgc ggatcggtac gacatgaatt caatcacgct cgcgtcttcg
      781 tcaaagctct caacgttccg ttctctggaa ggggggagcc tttgcttccg ctccatagtg
      841 aatgcctccg ggctactacg caacaattgg ccgccttggc atcaacttgc tattgcttgt
      901 tatcgatgcc ccggcggccc actaccgaag ctttagcacg ctctgtaaat tcgtccaaaa
      961 ggcggcaatc gacagcgtga attgggaaat atcttggttt tattcggacg aaatcggcaa
     1021 atcattcaag aatttggacg ttttgcccaa aatattcgca gcccccgcgt aattatgacc
     1081 agccgatcca ctcagggatg caggatcacc ttacccatcg ctttccgacc ggcgagcacc
     1141 ttcaacgcat cggcggtttg cgccagcggg aacgtccggt cgacgtgcga agagattttg
     1201 ccttctgcgg tccacttcac cagcttttgc agattggcgc ggtttttctc cgcattgagc
     1261 cgcgcccagg cgccccagaa cacgccgcgg atatcgcagc ctttcaagag cgcgagattg
     1321 agcggcatct tcggaatctc gccggcggca aatccaatca cgagaaagcg gccttcccat
     1381 gcgatcgaac gcagcgcagc ctcggcatag ctgccgccta cggggtcgaa aatgatatcg
     1441 acgcccttgc cgcccgtcag ccgccgcaag ccctccttga ggtcttccgt ggcgtagttc
     1501 aatccgagtt cggcgccgtg cgctttggcg aattcgagct tttcgtccga tgatgcgcag
     1561 gcgatcactt tcaggcccat cagtttgccg agttcacatg ccgccaagcc ggtcccgccg
     1621 gccgcgccaa gcaccgccat ggtctcgcca ggcttgaggc tggcgcgatc ttccagcgca
     1681 tgcagtgcgg tgccgtagat gatgatgatg ccggcggctc gatcgaaatc cagattgtcg
     1741 ggaattttga cgatggtatt cgccggcagc gcgatctttt cgcgggcgcc gttgtggccg
     1801 cacgacgcca ccacgcggtc gccgactttg aggtcggtga cgccggcgcc gacgctttca
     1861 atcacgcccg cgacctctgc cgccggcgaa aacggaaacg gcggcttgat ctggtacttg
     1921 ccctggatca tcaagaggtc gaagaaattc agcgccgccg acttgatggc gatcaccgcc
     1981 tgcccggctt cggccaccgg atcgggcaca tcggccagta caagttcgtc gggaccgcaa
     2041 tattgcgagc agagaatggc tttcatggac acacctggga atgggcgatg gataccgggc
     2101 tggacgcttc ttgccggatt tggagccggg ctacaatctg attcggacgc ccgatcctca
     2161 tgccgaggag gcagagaatt ttacgtcatt ccggggagcg ccaaagggtc cgcgcaaagc
     2221 gcggcccgat gacaggctcc gcgcgaacct cagatgtgca attgcacatc ggggaatctc
     2281 gagtttacag gatcactaac ctctggattc aggatccagc gctacgcgct ggtccggaat
     2341 gaccggagta atgggcaaag gatcgaccga tgtttgaaaa aggcctgttg gcgggaaagc
     2401 ggatattggt caccggcggc ggctccgggc tgggcgcggc gatgggacgc cgcttcgtcg
     2461 agctcggcgc cgaactgatc atctgcggcc ggcgccttga actgctggag gcgacagccg
     2521 cgcagatgcg cggcgatttc gccggcaagg taactgcgat cagatgcgac atccgcgaag
     2581 gtgctgccgt cgatgccatg atggacgcga tctggggcga cgtgccgctc gacgtgctgg
     2641 tcaacaatgc cgccgcgacc ttcatcgcgc agaccgagca gctgtcgttt cgtgccgcgg
     2701 atgcgatcct cgcgccgacg ctgcacggcg cgatgtactg cacgctcggt gcgggcaagc
     2761 gctggatcga ggcgggacac agcggagtgg tgctcagcat cctttcgacc tcgaccatca
     2821 ccggccgcgc cttcacggtg ccctccgcca tggcgaagtc cgccattctc gcgatgacca
     2881 aaagcctcgc ggtggagtgg ggacccaagg gcatccgcac ggtcgcgatt gcgcccggag
     2941 cgtttccgac ggcgggtgca tcgggtcagc ttcggcccga aggccgcgat gaagggtggg
     3001 cctccagaaa tccgctcggt cgggtcggcg aacaccgcga gcttgccgat ctggcgagtt
     3061 tcctgatttc cgattcggct ggctacatca acggcgagat ggtggtgcag gatggcggcg
     3121 cgcatttacg cggttccggc gccgaggacc tgctgcaatg gagcgacgcg cagtgggaaa
     3181 agcagcggtc ggcgcggtcg aaaagctgaa aacgaccagc gccgcatgcg ggcgcgccgg
     3241 aaatccggac gtgccgcttc agagatcgct aacttgcctt cccaaaagga aatttcccgg
     3301 gtatccatcc gccacgggac aacgggctat cgggccatct tccagtcaca atgatgaggg
     3361 agccacgctg gatcatgtcc gtggggcaaa tgctgacaac tctggcggcg agtgtgctgg
     3421 tgtgcggggc cgtgatttcc gcgcaggcac agggtgcctc gtcgaaaaat gcgaccaagc
     3481 cgacgtcttc caaacccgag gcaaaatcgg aagccaagtc cgccgctgct gttgcaggcg
     3541 gtgccgagcc gacactgatc ggccagttcg gcacctgggg ggcttacacc gcgatgccga
     3601 acggtaagaa ggtgtgcttc gcgcttgcca agccctcctc ctcgaaaacc aatccgccga
     3661 accgtccgcg cgacccggcc tatgcgttcg tctcgacgcg tccggccgaa aaagtcgtta
     3721 acgaagtctc gatcatgatc ggctacgcgc tgaagccagg ctcggaatcg acgctggagg
     3781 tcggcggcgc ctcttacgcg atgtataccc agggcgatgg catctggatc aagaacgccg
     3841 ccgaggaaga gcggatggtg gatgccatgc gcaaggccgc cgatgccacc gtcaagggcg
     3901 tgtcggcgaa gggcacagag accacggaca cgttctcgct gaaggggctt tcccaggcgc
     3961 tcgacaaact ggcgcaggat tgcaagcgct agggcatgat ccggaaaagt gggtaccggt
     4021 tttccgaaaa gatcatgctc aaacaaaaag atagagcggg atgacgattt gaagaaaagt
     4081 catcccactc tagcgcgttt ttaaacgaag catgccctcg gacttgatcc gggggtggaa
     4141 accggtccgc gtcaagaaaa cgcgtcaaat caagaattta gagccccggt tcgattctat
     4201 cgaaacgagg ctctgggcgg ctttcacgtc tagattgacg catttggtgg ctttccagct
     4261 gaccgtggaa ggcctatctg atgttcgatg aacgagacaa tgactagcat ttcaatcgaa
     4321 gccggttccg gcgcaaaccc gcccctggaa aagatcgcgc tcgaaaccta cgtgccgccg
     4381 gcaaaaccct cgctggtcgg actgtcgcgt gcggagatcg ccgaacagct cggcgccatc
     4441 ggtgtcgctc cggcgcagcg caagatgcgg gtgcagcagc tgtggcactg gatctatgtc
     4501 cgcggcgcga agaaattcga cgagatgacc agcatttcca gggaaatgcg tgcgcagctc
     4561 gattcccatt tcacggtcgc gcggcccgag gtggtcgccg agcaggtttc caatgacggc
     4621 acccgcaagt ggctgctgcg cctgccgagc ggcgatcgtg tcgaaaaagc ccatgaggtg
     4681 gagtgcgtct atattccgga gaccgaccgc ggcacgcttt gcgtatcctc gcaggtcggc
     4741 tgcacgctga attgctcgtt ctgccatacc ggcacgcagc ggctggtgcg caacctgacc
     4801 gcaggtgaaa tcgtcggcca ggtcatggtg gcgcgcgatc gcctcaatga ctgggctggc
     4861 cgcatcacgc cgaccggcag ccgcctcgtc accaacgtgg tgatgatggg catgggcgag
     4921 cccttgtata atttcgacgc ggtgcgcgat gcgcttctga tcgtcgccga caatgagggc
     4981 atcggcattt cccgccgccg catcacgctg tcgacctcgg gcgtggtgcc gaacatcatc
     5041 cggaccggcg atgagatcgg ggtcatgctg gcgatctcgc tgcatgcggt gcgcgacgaa
     5101 ttgcgcaacg aactggtgcc gctgaaccgc aaatatccga ttgccgaatt gctgcaggcc
     5161 tgtcgcgact atccgggatc gtcgaacgcg cggcgtatca ccttcgaata tgtgatgctg
     5221 aaaggcgtca acgattcgct ggaggacgcc aagctcctgg tcaagatgct gaaggggatc
     5281 ccggccaaga tcaatctcat tccgttcaat ccgtggccgg gcactcgcta cgagtgctcg
     5341 gactgggacc agatcgaaaa gttttccgaa tatattttca acgccggtta ctcctcgccg
     5401 gtgcgcacgc cgcgcggccg cgatatcctt gccgcctgcg gccagctcaa atccgagacc
     5461 gagaaattgt cggtgcgcga gcggcaggcg ctgcgcgcca tggcgatgac ggattgaggg
     5521 catggagctg aagtcgtcat ggccgggcat agccgtcgga aggacggcgt cgcttcggct
     5581 cgcctatgcc cggccatcca cgcctttaca gagtttcgac aaagcaatgc gtggatgccc
     5641 gggacaagcc agggcatgac ggctgagagg ttcgtgccgg tgttgatggg tctagtggca
     5701 tggcattgat cggccgcctg ttcgcgatct tgttcggatt cctcgccgcg tgcctggtgg
     5761 ccggaatgat cgtggtcgca gcagtgctgt ttccggaatt cagcgatttt gcgagcggcc
     5821 cgatcgatcc atccgcgctg aacatcgtgc tcggcttcgg ctttgtcttc gtcagcgggt
     5881 ttgcgctctt gccggcgatg atcgtggtcg cgatcaccga agcgttctat atcaggggcg
     5941 tgctgaccta cgcggtcggc ggcggcgtgg tcggcctggc gtgctatctc ggcctgatcc
     6001 cgttcgatcc cgcgacactt tccttcgatg gcatcgtccg gcgtcatctc gagatcatga
     6061 ccggtgccgg aatcgtcgcg ggcctcgtct actggatgat cgcgggccgc aacgccggcg
     6121 cctggcgcga gccgccgcgc ccgctgccgc ctctgccgcc gttgccgtcg cattcaccgt
     6181 ccccgccgca gcattctccg ccatcatcct gaggtgcggc ctcttggccg ggtctcgaag
     6241 gatgagctac gtgagaagga tgagttacat gaatcgtggc catccttcga ggctcgcaag
     6301 ggctcgcacc tcccgagtga ccgcaaacgc gctcatccgg ggatgacgac agcatcatcc
     6361 gcccttttca tctccaacct cctcggctaa atcccgcgcc atgaaccgat caggacttct
     6421 caccgcgctg gccctggcgc tcgtcatcgg attgctgttc gggatctatc ccgagctcga
     6481 tctcaaactt gccgccctgt tttacgacgg ggcgacccaa tcctttccgc taaagctcaa
     6541 tgcgatggca gcacttgcgc gcgacggcgc gatgtggatc gcgtggggga tcgcgctgcc
     6601 ggcgatcgtc gcattggcgg tgaagctggt acggccggat cggccactgt tgatgtcagg
     6661 gcgcgcgatt atcttcctgc tcgcgaccct cacgctgtcc gccggcattc tcaccaatct
     6721 taccttcaag agctattggg gccggccgcg cccggtggtg gtgacgcagt tcagcggcga
     6781 tctgcctttc gtgccgtggt gggatccgcg cggcggctgc agccgcaact gctcgttttt
     6841 ctccggcgaa ggcgccaccg cgttctggac gcttgcgccc gccgcgctga cgccgccggc
     6901 ctggcggccg ctggcctatg tcgccgcgac gctgttcggg gtcgccacca gcgcgctgcg
     6961 gatggcattc ggcgggcact tctttaccga cgtcgccgcc gctgggctgg tgacgtttct
     7021 ggtggtctgg ctggcgtacg gctatattta ccgctggccg tccacccgtc tgtccgacga
     7081 gagcatcgac gccgcgcttt cgcggcttgc ctggccgggg tatcgcctcc ttcaaaaatg
     7141 gcgcggtcgc gatgtcgggc ctgcttcgtc ggtgtgagcg cttgcgcgcc ggacctttct
     7201 gcctgtaatc aatttgcgcg tgttgcacgc aaattccaac atccagttcg acaggatttt
     7261 catgagcaag gacgtcaaga aggtggtgct cgcttattcg ggcggcctcg acacctcgat
     7321 catcttgaag tggctgcaga ccacttacgg ctgcgaagtc gtgaccttca ccgccgacct
     7381 cggccagggc gaggagctgg agccggcgcg ccagaaggcg ctgctgctcg gcatcaagcc
     7441 ggagaatatc ttcatcgagg atgtgcgcga ggaattcgtc cgcgactatg tgtttccgat
     7501 gttccgcgcc aacgcggtgt acgaagggca gtatctgctg ggcacctcga tcgcgcggcc
     7561 attgattgcc aagaagcaga tcgaactcgc cgagaagctc ggtgccgacg cggtctccca
     7621 tggcgccacc ggcaagggca acgaccaggt ccgctacgag ctcgcttatt ttgcgctgaa
     7681 gcccgacatc accatcatcg cgccgtggcg cgaatgggat ttgcgttcgc gcgagcaact
     7741 gatcgcgttc gccgaagcgc atcagatacc gatcgccaag gacaagcgcg gcgaggcgcc
     7801 gttctcggtc gacgccaatc tcttgcacac atcatccgaa ggcaaggtgc tggaggatcc
     7861 cgcgctggaa gtgcccgatt acgtctattc gcgcaccatc gatccggaga aggcgccgga
     7921 tcgggcgacc accatcacca tcgatttcga gaagggcgac gcgaccgcga tcgacggcaa
     7981 gaagctgtcg cccgcgacgc tattgacaaa actcaacgag ctcggccgcg ccaacggcat
     8041 cggccggctt gatctcgtgg aaaatcgttt cgtcggcatg aagtcgcgcg gcatgtacga
     8101 gacgccgggc ggcaccattt tgctggtggc gcatcgcggc atcgaatcga tcacgctcga
     8161 tcgcggtgcg gcgcatctca aggacgagct gatgccgaaa tatgccgagc tggtctacaa
     8221 cggcttctgg ttcgcgccgg agcgcgagat gctgcaggcg gcgatcgatc acagccagca
     8281 atacgtcacc ggccgcgtgc gcctgaagct ctacaagggc aacgtcatca tggtcggccg
     8341 cgagagcgaa tattcgctgt acgaccagga tctcgtcacc ttcgaggaag gtgcggtcgc
     8401 ctacgatcac cgcgacgccg ccggcttcat caagctcaac gccttgcggc tgcgcacgct
     8461 cggccagcgc aagaagaagc tggggctgta gggactcgcg catctccacg tcgtcccggc
     8521 gaacgccggg accccagcgt gagcgcagtt gcgctcatcg cggcggcgcg gagtctcgct
     8581 tcggcactgg agcagatacc ttcttcaaca agctaagccg ggggttatgg atcccggcgt
     8641 tcgccgggac gactcgtgga ataacgacgt ccaaacaaaa atggccggga tttctcccgg
     8701 ccatttctgt tcagcggatg ccgcctcacc tcggcggtgc gatgcccggt ggcggcggcg
     8761 gcagcgatgc cgttccgccg ttgagcaacg gcgtgatgcg acggatggtg acgcgccggt
     8821 tgatgcggct tggcccgtcg gtctgttctt tgagatattg cttgccgtat ccttgcgagg
     8881 tcaggttttc cgctggcacc tggaattgct gggtcagcaa ttgggctgcg gattcggcgc
     8941 ggcgatccga cagcgacaga ttgtcgacgt ccgatcccac cgcgtcggta tgaccttcga
     9001 tcaggaacac ctcgcgcggg ttgcgcgaga tcgcccggtt gaggccgtcc gcgatcgact
     9061 gcagcttcgc cacctgatcg ggcggaattt cccacgatcc ggtttcgaag ttgatgctgt
     9121 tgaggtcgat gctcggcatc aactgccgta ccgacgggct gtagcggatt tcgtcgagcg
     9181 aatagcgccg ctcgatccgg tctaccggcg gcgcctccat ggtctcgtaa atcagatcgg
     9241 gcggcgcgtc ctcggcgtcg acgatgtaac ggtcgtacgg aatgcggatc accggcggcg
     9301 gcaggtcgac atagaaaccg cccaccgcgc gaggatcgcg gtagctattg tcgatgatga
     9361 tgatctcgcg gccccgctca tcccggcgaa tccggcgcag catctggccg tcgcggccgt
     9421 tcacggtgat gatctggctg ccgtcgggac ggatcacgat ggtgcgcgtt tcgccgccga
     9481 cctgctcggt ccggatgtcg cgcgcgccgt agcggaagcg atcctcttcg ttgtggcgaa
     9541 tgaaggattg gccgccggga tcgacaacga tgacgcggcc gggctcgtta aagaccgtgc
     9601 ggccgccctc ttgggtttcc cggcgtgctc ccctgaaatc atccatgcgg cgcggccccg
     9661 gtgcctgcgc acccggagcg atcggcgcca aggcttgggc cggcggcggg gcggccggaa
     9721 tcggcgcgct gacagttggc ggcgcgtgac ggactaatgg cgcggcgcct gcaggcgctc
     9781 cgggcggcag cgacgtctgt ccgccggccc cacccggtgc aggagcggca tttggcgcag
     9841 cgttcggagg cggaccatta cgcggcggcg gcggcggcgt cgtacccgcg cccgcgggtg
     9901 gcggcgcagt ctgggtcggc ggagcggccg ccggcagggc acccgcggca ggcggctgct
     9961 ttgcgccggc gggcggcggc gcagtctggg tcggcggggc ggccgccggc ggggcgcccg
    10021 cggcaggcgg ctgttttgcg ccggcgggcg gtggcagtac cggtgccggt gcattttttt
    10081 gctgaagctg ttccggcgca tttctttgct gaagctgcgg cgcaggcgca gcgctcggtg
    10141 gagctggagg tgcagcactt ggcggagccg gaggttttgg cggggcaggc ggtggcgccg
    10201 ctgccggagg agtcggcggc gcgggatgcg gcggcggagc tgccgcgggt ggcgggacat
    10261 gcgggggtgg cgccggaggc ggcggaggag gcgcggccgg aggcggcggc gtgggtcgcg
    10321 gcggcggtgc cgcggctgga ggcggcggcg gcgcggggcg cggcgggggt gctgcagccg
    10381 ggggcggcgg tgcaggtcgg ggtggcggag gtggcgcggc agccggaggc gcaggatgcg
    10441 gcggaggtgc tgcggccggc ggcgccgggt gtgcgggtgc gggcgcgggc gcgcccttcg
    10501 gcggggcttt cggttttccg tcgggaccga cttcaccgcc ggcctgcgcc atcacctgcg
    10561 gcgcggtttg cgcgtgcgat ggcaaggctg tgatttgcgt cgccgtaaga gctgacgtgg
    10621 caagcaacac gagacgaaga tttgtcatga tggtctggat ccccggaaaa tttttggcaa
    10681 caacgcagtc ctgttaagaa aaacgcagtc ggtctggcga tcaacgaaca cgcgttaggc
    10741 cggattgtgg ccgcccccaa aaccgaattt tgtttttatt ttccaacaag gggaacgttc
    10801 tgacggcctt gttcattgcg cattcaggcc attatttcaa accgtagttt cacattgtcc
    10861 cctgcggcat atctgcggca cccggatcca gatctggccc ttcaccggac aaatcgccga
    10921 tcccggcgtc ggctgcaggc gaaggcggac ccggtccacc cggtccccgc accggcagtt
    10981 catccggcat gtttcctggc aattcttccg gtcgcggcgg ttcgccaggc tcctgcatcg
    11041 gcggcggcac ctcgggtcgt gggcttcccg gcgggctttc ctgcggcggc tcggacggtt
    11101 gccccggctt gatcggcgga tcctcttgcg gttcgatcgg caccacagcg ccaccttcat
    11161 attgtcgctc ctctggcttc atagcgtttt cgagcgaagc atgccctcgg actagatccg
    11221 ggggtggaaa ccggttcgcg tgaagaaaac gcgttaaaca agaatctaga gccgtacagg
    11281 ctctaggagc gataacgacg ccgataccgc gatgttcccg ggccaaagca ggttggaacc
    11341 ggtcactcgg gcgcgtggca cacggcttcg atgttgtgtc catcgggatc gagcacgaag
    11401 gcgccataat aattggggtg gtagtgggcg cgaatgccgg gcgcgccgtt atcgcgtccg
    11461 cccgccgcca tcgccgtctg gtagaaagcc gcaacggtgg cgcggtcctt tgccacgatg
    11521 gcgacatgca gcggcttgtt caatccgcct tcgccgccga tccagaaatc cggtttgccg
    11581 ttggcgccaa agccggccgc gggatcatga ccggtctgct cttgcgtcac ttccatgatg
    11641 aggctgtagc caagcggcgc cagcgccttc gtataaaagg ccttggcgcg cgcatagtcg
    11701 gaaaccggaa atccgatgtg gtcgatcatc caactctcct gcggttcgag cgcgggcggg
    11761 aactcagccg cgcaccaata gcgtgttgct gcgggtcttg ccggtcgcgg catagccgcg
    11821 cgcaaccatg tctgcaatac agtcatcgag ccattcgttc cgcgacagat gctcgatcac
    11881 caccgcgcgc ggccacagcg agggcggcgc ctctttgaaa aagccggtca gcacgcggtc
    11941 ctcaaaaccc tcgacatcga tcttcaacgc atcgacgtgg cctatcccgg cgtcgccgag
    12001 aatacgctgc agccgcaacg acggcacctt gatcgccttg cccgaatggt cgccgctgac
    12061 gatatggctg gcgccgaggt tgtcgccgtc ggtctcgatc atcaattcgc cgtcggaagg
    12121 cccggctgcc gcggccacca gcgttgcctg cgtggtgccg gacgcggcac ggttgaacgc
    12181 cagccgcgca tgggtgatgg gatgcggctc gatcgcgatc acttttccgc ttgcaccgac
    12241 atgacgcgcc agcaccatgg cgtaggtgcc gacattggcg ccgacatcga cgaacacgcc
    12301 gccgaccggg gtatgcgcgc gcaggaaatc cagctcctcg acattgtaat cgggattgaa
    12361 caaggcgccg cgctcggtgg cgctggcctg atgatagaat cgaaacgccg cgccctgata
    12421 ctggacatcg accggacccg cgcgcagcag gttgaccagc cgcgacatca tcgggcggaa
    12481 cgcaccgcgt ttcagccgcg agcgttgtgc gagcgcgatg acggcagctt gcgctgatgt
    12541 cggcgcgaaa acgccgaacg gcggtggcgc cggatcgttg gccgcggcga ggccgggtgg
    12601 cagtgtgggt atcgtcaaac catcctcgcg acgcgtttca aggaagcggc ggcatgcata
    12661 gctggttttt cgggcgactc caacgttgtg tcttgcgttg tgtcttgggt ttgcgcgtca
    12721 tcccggcggc ttcgccggcc cgacgaaagg ctccggcgag cctcgaagca ccgtgacgag
    12781 aatgtggcgc atccttcgag gtcgtcgcag gcgcgacgtc gcctcaggat gacgaaagga
    12841 ttcgcggcaa ggccgtccaa tcaatccgga tcgataaatt gatagtcgat tcgaacccgg
    12901 ccatcttctg ccagcagcaa gacgatcaag ccggttgctg cgatcgcgcc accgctgttc
    12961 gggaccatgc gccaggtgaa cttgaccatg tcgcgcaatt gctcgacgcc accggcgagc
    13021 tcgaaggtgt agttccgctc ggccacgttg gtttgatgcg aggtggcgac gcgcagttcg
    13081 agatcggcgt ggccgtgcac ttctcgggcc cggacataat gcgtgccatt ctcgatccag
    13141 agtccggcaa cctgcgaacg gcgggcggcc cgatcggtct cattccagat gtggacataa
    13201 cgccgggcga gatcttctgc gtgggacatg atgaggctct atcttcgggc tttattgctg
    13261 catggctgcc cgtttcttcg ggctgtacga gagacgatag gccgtggtat ctggacgatg
    13321 aatagctgtt tggctcagat tgttgtccga tcgtccaaat catagccggc gaggggaggg
    13381 cgtatggatc cgctggacga tgcgttttca gccatgcggg tgcgcgaatc gctctacgcg
    13441 cgggtcgatg ccggtgcgcc atgggccatc aggttcagga aaggaaaggc ggcaaggttc
    13501 ggtctcgtgg ttgccggctc atgctggctc accaccgagg cgcctgcgca atcgatcccg
    13561 cttgcatcag gcgattgcta cgtgatcctc gacggctcga cttacacgct tggcgatgat
    13621 ccgcgctcgc ccgcgttgaa ttgcttcgat gtcgttcccc ggctggtcga tggcgccgtc
    13681 agcctcggag gcggcggcgc cgcggccacc gtggtgaccg ggtggttcgt ctacgacgaa
    13741 ctcggggcgc gggcgttgat ggcgctgctg ccgcgcgtgc tgcacacacg cgtcgatggt
    13801 taccggaccg atattctgaa ggcgacgctg gaattgctgg caaaggagac cgagcgcccc
    13861 ggcgtcggct ccggcgtcgt catcagcggg ttagcggata ttttgttcgt gcaggcgatc
    13921 cgcagtcatc tcaacaatac cagcgagaac gatgtcggct ggctcgcggc attatccgac
    13981 aggcgcatcg ggcttgcgat gcgtgcgctg cacggcaagc cggccgacgc ctggacggtg
    14041 gcgaaactgg cttcgctggc gaacatgtcg cgctcggctt tcgccgcgcg tttcaaggca
    14101 aggctggggg aagcgccgct ggaatatctg acgcggtggc gcatgttccg cgccggcgtg
    14161 ctgctgcgaa gcacacagcg ctcgctggcc gagatcgcaa acgaggtcgg atacgaaagc
    14221 gacgcggcgc tcagcaaagc tttccatcgc gtcgtcggca tgacgcccgg cgcattccgc
    14281 aagcagaacg cgccggcgcc ggaaaacccg cggcgggcag tggcctgacc cgccgaccta
    14341 ggcaggcagc acatggaatc cggcgcgcgg aaaatgcacg atcacctcgc cagcgcgcgg
    14401 gtcatgcctg cgaacggcga tgcgctgtga cgagagcgcg acgatttcgc cgctcacttt
    14461 gatcttgccg tagtcgtcgg gcatgacctc gacccggtcg ccgggcttgc ggccgttcgg
    14521 atcgttggga tcggccagaa tggcggtttg cggcgtggcg cttgcggcga cgtcgagcgc
    14581 ggcggaggtc gatatctcgc ggaaacgacc gtgaccgacg ttgcgcatgc gcatttccca
    14641 tgcccgcgtg ttgccgaact ccgcgagcat ctcgtcggca ttgggcaggt gggcgcgcac
    14701 gtaccagacg ttcatgtagg cgttcacatc gcaaaggccg gctctgtcgc cgctcatcca
    14761 cgcccgtccg tcgccgagct gggtctcgac ccattgcagg tgagcgcgaa actggtcgcg
    14821 catttgcggg atcgccgccg tcatggccgg gacatcgaac ttggcgccgc gcagcttttc
    14881 gcggtcggcg ataaaatcct gcgggacctg gtccgccagc gagccgaaca cgagattcac
    14941 tgtgttctgg aagaacgacc gatcggtcca catcgcggtg ccccagccga tgccttgata
    15001 tccgttcggg aacagggtcg gttccggaaa ccgccgctcc agttcgcgaa tgatgcattg
    15061 tgtgtcgcaa tagatgtcgg ccccgatctg catcaccggc gtgcgccgat agcccccggt
    15121 caacggcatc aggtcaggac gcggcatgat tcgcgagata agaaccgaga tccattcgat
    15181 gttcttgagg ccgaagacga cgcgcacctt ctccgagaac ggcgactttt cgaaatgatg
    15241 caggataatg ggatgcgcgg tcgtcatcat gcgagccctt caggctttgc tcgtgcttac
    15301 gtcaagcccg gccatgacga gaagacttaa atctgccgct cgaccatctt cagcttgagc
    15361 tcggcgatgg cctcggaagg attgaggctc ttcgggcacg ccttggcgca gttcatgatg
    15421 gtgtggcagc gatagagccg gaacgggtct tcgagattgt cgagccgctc gccggtggc
//
LOCUS       DASWGM010000065         4123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1944140,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000065 DASWGM010000000
VERSION     DASWGM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944140"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1432
                     /locus_tag="VGA15_02170"
     CDS             161..1432
                     /locus_tag="VGA15_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9586539.1"
                     /translation="MHGTIDLVQNSDQKAARERAYSIPLEQFDPGDPELFRSDTFWPY
                     FDRLRKEDPVHYCKDSVFGPYWSVTKYNDIMDIETNHSVFSSAASLGGITIRDIAPDL
                     RRESFIAMDQPRHGAQRKTVAPMFTPTHLDQLATNIRQRSAECLDNLPKNDVFDWVDR
                     VSIELTTQMLAVLFDFPWEDRRKLTRWSDVATTIPGPDGVVATEDARQAELLECATYF
                     GKLWKERANQPPKSDLLSMMVHSEATRDMDPKNFLGNLILLIVGGNDTTRNTLSGSVY
                     ALNKNPDQYRKLRENPALIDSFVPEVIRWQTPLAHMRRTALEEFEFRGRQIKKDDKVV
                     MWYVSGNRDDEVIERPYDFIIDRARPRTHISFGFGIHRCVGLRLAELQLKIIWEEILK
                     RFDNIEVAGEPKRVYSSFVKGYETLPVRIAG"
     gene            1465..2739
                     /locus_tag="VGA15_02175"
     CDS             1465..2739
                     /locus_tag="VGA15_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9586540.1"
                     /translation="MNIRTSVNADKAELLRLAREEAYSTPLKDFHPGAPRLFQNDTLW
                     PWFERLRKEEPVHYCTNAPIEPYWSVTKYNDIMHVDTNQGIFSSDASLGGISIRDAPA
                     GYDWPSFIAMDQPRHSAQRKTVSPMFTPTHLDELAVLIRERAGKVLDALPRNETFNFV
                     DRVSIELTTQMLATLFDFPWEERRKLTRWSDIATALPKSMIVESAEQRRTEMDECAAY
                     FTKLWNERVNAEPKNDLISMMAHSDATRNMDPDNLMGNIILLIVGGNDTTRNTMSGSV
                     LALNENPDQYQKLRDNPALIDSMVPEVIRWQTPLAHMRRTALVDAEIGGKKIRKGDRV
                     VMWYVSGNRDEEGIENPNDFIVDRARPRTHLSFGFGIHRCVGMRLAELQLKIVWQEML
                     KRFDRIEVVGEPKRVYSSFVRGIESLPVRIPA"
     gene            2874..3311
                     /locus_tag="VGA15_02180"
     CDS             2874..3311
                     /locus_tag="VGA15_02180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9586541.1"
                     /translation="MSAALNPPSLAETINAKGFSHAAGFRIVAVEPGRAEVALARRDD
                     LVQFFGHFHGGVITALADHAAGIAVTSGLPVGRIGVTVEIKVNFLSPAAGHELVARAK
                     TLKMSGSIGVATVEVFSKDGASETLCAFATATMRALDLPAEFR"
     gene            3308..>4123
                     /locus_tag="VGA15_02185"
     CDS             3308..>4123
                     /locus_tag="VGA15_02185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9586542.1"
                     /translation="MTATEPHDEFADIRDAVAKLCAQFPGEYWRKLDREMAYPSAFVD
                     ALTEAGYLSVLIPEEYGGAGLKLSAAAAILEEIQRAGCNGGGCHAQMYTMGTLLRHGN
                     DQQKSKWLPKIASGELRLQAFGVTEPSSGTDTSSLKTFARRDGNNGYVVNGQKIWTSR
                     AEYSDLMILLARTTPKKQSQKRTDGLSVFIVDMKAAKGNGLSISPIRTMMNHSTTEVF
                     FTDLLVPAENLIGEEGKGFRYILSGMNAERILIASECVGDAKWFIAKASAYARE"
BASE COUNT          881 a         1283 c         1208 g          751 t
ORIGIN      
        1 tcacgggccg aatatgctga aaagggtgca ccggccggaa tgctactttg acgcgccgga
       61 aggctcaaac tctcccttgc cctgccacac gcaacgggat gtaattcgaa gccaaagcct
      121 ctggaatcaa ccacggagga aacaaacagg gagttgcccg atgcatggga cgattgatct
      181 cgttcaaaat tccgatcaaa aagccgctcg ggagcgagcc tattcgattc cgcttgagca
      241 atttgatccc ggcgatcccg aattgtttcg cagcgatacg ttctggccct atttcgatcg
      301 gcttcgcaag gaagacccgg tccattattg caaggacagc gtgttcgggc cttactggtc
      361 ggtgacaaaa tacaacgaca tcatggacat cgagacaaac cattcggtgt tctcgtcggc
      421 cgcatcgctc ggcggcataa ccatccgcga catcgcgccc gacctgcgcc gcgaaagctt
      481 catcgccatg gatcagccgc gccatggcgc gcagcgcaag accgtcgcgc cgatgttcac
      541 gccgacgcat ctcgatcaac tcgcaaccaa catccggcag cggtcggctg aatgccttga
      601 caatctgccg aagaacgatg tgttcgactg ggtcgacagg gtttcgatcg aactgaccac
      661 ccagatgctc gccgtgctgt tcgatttccc ctgggaagac cgccgcaaac tgacgcgctg
      721 gtccgacgtc gccacaacga ttcccggccc ggatggcgtc gtcgcgacgg aagacgcgcg
      781 acaggccgaa ctgctggaat gcgcgacata tttcgggaag ctctggaaag agcgcgccaa
      841 tcagccgcca aagagcgatc tattgtcgat gatggttcac agcgaagcca cacgcgacat
      901 ggacccgaag aatttcctcg gcaacctgat cctgctgatc gtcggcggca acgataccac
      961 gcgcaacaca ctgtcgggca gcgtctatgc attgaacaaa aatccggatc agtaccgcaa
     1021 gctgcgcgag aatccggcgc tgatcgacag tttcgtgccg gaggtgatcc gctggcagac
     1081 gccactggcg catatgcgcc ggaccgcgct cgaggaattc gagttccgtg gcaggcagat
     1141 caagaaagat gacaaggtcg tgatgtggta cgtctcgggc aaccgcgacg acgaggtcat
     1201 cgagcgtcct tacgacttca tcattgaccg ggcccggccg cgtacccaca tctccttcgg
     1261 cttcggtatt catcggtgcg ttgggctcag attagccgag cttcaattga agataatctg
     1321 ggaagaaatc ctgaagcggt tcgacaatat tgaggtggcc ggcgagccca agcgggtata
     1381 ttcgagcttc gtcaagggct acgagaccct gcccgttcgc atcgccggct gactgcggcg
     1441 cgccaaattt ggaatcgatc aacgatgaac atccggacat cagtcaacgc cgacaaggcc
     1501 gaacttttgc gcctggcccg tgaggaggcc tattccaccc cgctgaagga ttttcatccc
     1561 ggcgcgccgc gactgttcca gaacgatacg ctgtggccgt ggttcgagcg gctgcgcaag
     1621 gaagaaccgg tgcattattg caccaacgcg ccgatcgaac cttattggtc ggtcaccaaa
     1681 tacaatgaca tcatgcatgt cgacaccaac cagggcatct tctcctcgga cgccagtctc
     1741 ggcggcatct cgatccgcga cgcgccggcg ggctatgact ggccgagttt catcgcgatg
     1801 gatcaaccgc ggcactctgc ccagcgcaaa accgtgtcgc cgatgttcac gccgacgcat
     1861 ctggacgaac tcgccgtcct gatccgcgaa cgcgccggca aggtgctcga cgccctgccg
     1921 cgtaacgaga ccttcaattt cgtcgatcgg gtgtcgatcg aactgaccac acagatgctg
     1981 gcgacgctgt ttgactttcc ctgggaagaa cggcgcaagc tgacgcgctg gtccgacatc
     2041 gcgaccgcgc tgcccaaaag catgatcgtc gaatcggccg aacagcgccg gaccgagatg
     2101 gacgaatgcg cggcctattt taccaaactc tggaatgagc gcgtcaatgc cgagcccaaa
     2161 aacgacctga tttcgatgat ggcgcacagc gatgccacgc gaaacatgga tccagacaat
     2221 ttgatgggca acatcatcct gctgatcgtc ggcggcaacg acaccacccg caacaccatg
     2281 agcggctcgg tgctggcact gaacgaaaac cccgaccagt accagaaact gcgcgacaat
     2341 ccggcgctga tcgacagcat ggtgccggag gtcattcgct ggcagacgcc gctggcgcat
     2401 atgcggcgca ccgcgctggt ggatgccgaa atcgggggca agaaaatccg caaaggcgac
     2461 cgggtcgtga tgtggtacgt ctcgggcaac cgcgacgaag agggtatcga gaaccccaac
     2521 gactttatcg tcgatcgcgc ccggccccgc acccatctgt cgttcggatt cggcattcat
     2581 cgctgcgtcg gcatgcggct cgccgagctg cagctgaaga tcgtgtggca ggagatgctc
     2641 aagcgctttg acaggatcga ggtggtgggc gagccgaagc gggtgtattc cagcttcgtg
     2701 cgcggcatcg aatccctgcc ggtacgcatt ccagcgtaag ggcgggcgtg cttgcgcttt
     2761 gcctgccccc cgtgctgaga tcgccggggc ggggaacggc atctgcgccg tgcccccgtt
     2821 cgccggatgc aagatatacc gccaagatat actgccatgg acagaccagc accttgagcg
     2881 ccgccctgaa cccgccgagc ctcgccgaga ccatcaacgc gaagggtttt agccacgccg
     2941 ccggtttccg cattgtcgcc gttgagccgg gccgcgccga agtcgccctg gcacgccggg
     3001 acgacctcgt gcagttcttc ggtcattttc acggcggggt cattaccgcg ctggccgacc
     3061 atgcggccgg aattgccgtt acgtcaggcc tgccggtggg ccggatcggg gttacggtcg
     3121 agatcaaggt caatttcctc tcgcccgcgg ccggccatga gcttgtggcc cgcgccaaaa
     3181 ccctgaaaat gtcggggtcg atcggggttg ccaccgtcga agtcttcagc aaagacggtg
     3241 cttccgaaac cctctgcgca ttcgctaccg ccaccatgcg cgccctcgat ctgccagccg
     3301 agttccgatg accgcaaccg aaccacacga cgaatttgcc gacatccgcg atgccgtcgc
     3361 caagctgtgc gcccagttcc ccggcgaata ctggcgcaag ctcgatcgcg agatggccta
     3421 tcccagcgcg ttcgtcgatg cgctgacgga agccggctat ctctccgtct tgattcccga
     3481 agaatatggc ggcgccggct tgaaactatc cgcggcggcg gcgatcctcg aggagatcca
     3541 gcgcgccggc tgcaatggcg gcggctgcca tgcccagatg tacaccatgg gcaccttgct
     3601 gcgacacggc aacgaccagc agaaatcgaa atggctgccg aagatcgcca gcggcgaatt
     3661 gcggctgcag gcgtttggcg tcaccgaacc gagcagcggc accgacacct cgtcgctgaa
     3721 gacgtttgcc aggcgcgacg gcaataacgg ttatgtcgtc aatggccaga agatctggac
     3781 cagccgcgcc gaatattccg acctgatgat cctgctggca cgcaccacgc cgaagaagca
     3841 gtcccaaaag cgcaccgacg gtttgtcggt gttcatcgtc gatatgaagg cggcgaaggg
     3901 taacgggctt tcgatcagcc cgatccgcac catgatgaac cattccacca cggaagtgtt
     3961 cttcaccgat cttcttgtgc cggcggaaaa cctgatcggc gaggaaggca agggctttcg
     4021 ctatatcctc tccggcatga atgccgagcg cattctgatc gcgtccgaat gcgtcggcga
     4081 tgccaaatgg ttcatcgcca aggcttccgc ctacgccagg gag
//
LOCUS       DASWGM010000066         6233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_558582,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000066 DASWGM010000000
VERSION     DASWGM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558582"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1533
                     /locus_tag="VGA15_02190"
     CDS             43..1533
                     /locus_tag="VGA15_02190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9586543.1"
                     /translation="MSETSLGMAARSDGADLRERSRLSFDLDVDICVVGAGLAGLTVA
                     LEAARLGASVAVLEGRHVGWNASGHHLGTVMPGFALPIGELIARIGFEDSRELWSLSK
                     QGADYVRANATEELMPGIALSEGALEVSNVEAGEQLISRLQMLSEDFETDVEGWQVDR
                     VRDQLKTDHYFHGVYYPKAFQIDGRKYVHGLAALTRRAGARIFEDTPVVSIDPSGIRK
                     RIVTPSARLRASHIVLAGNIHLGEPLRRLSETLLPVWRYAAVTAPLGERLADVVRFQG
                     SVTDTVGIDHFRIVDGDRLMWASPETTWAARPERFAGPIARRIRTIFPRLGTTEIAEV
                     WGGAVGETVHGMPQIGQLRRGLWVTSGFGRQGLNTSAMAGQLIARSILWGDERWRLFS
                     PFELVWAGGPTGRVAGHLVGMWGRGSSAAAGALARYRERAQARERIREARLLAANRQA
                     GTRPPRRPTPPVRPRPMRPRPPVQTNPGEGEGFPHPNPPPQAGGGT"
     gene            1754..2254
                     /gene="msrB"
                     /locus_tag="VGA15_02195"
     CDS             1754..2254
                     /gene="msrB"
                     /locus_tag="VGA15_02195"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX9586544.1"
                     /translation="MIDRRILLASVASLLGLAAFRWLRASPANAAEKFEIEKTDAEWR
                     AQLTPQQYEILRKEGTERPGSSPLLKEHRKGIFACAGCDLPLFSSETKFESGTGWPSF
                     YQPLENAVGKTEDRTFGVLRTEIHCRRCGGHLGHVFDDGPKPTGLRYCMDGFALVFHP
                     AAPSAT"
     gene            2448..4280
                     /locus_tag="VGA15_02200"
     CDS             2448..4280
                     /locus_tag="VGA15_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HEX9586545.1"
                     /translation="MGIFDALNTAVGGLQAQSFALQNISGNIANASTTGYKGIETSFE
                     DLIPDSTSPSRQVAGGVTAFAQATITTQGTVSVTTVATNMAINGDGFFSVQKATGTTD
                     NLPVFNGVTDYTRRGDFQVNANGNLVNGAGYYLMGVGVDPKTGNPLGNVPQVLQFQNN
                     FIPAQSTTSIKYAANLPSTPTTAVSSTAAAGTINALGGLNPFDFSNNPLPLGTPATAP
                     GDNTVTGTAEFNQLATPVAITAATKLSGAAGTDSVTPSFAAGDTITIDGATYTFSASP
                     TNLATNTINVGDTVGTLMGVIQTATGVAPSITGGAITLHTGTAANLTITTGGTVTATN
                     ALAALGFSGGGVTLSRTPAGTVGTGTVIGNDLQTFLNESISGGAVTAYDSTGTAVNVQ
                     LRWAKTDSAALGGSHTNTWNLFYQSNPNATGSQTAWVNAGTNFTFGPTGVLTSPATPA
                     ITIPNVSVGNQSLGNLSFSMGAGTLTQFASTGGTVTINTITQNGFAAGQLQSVAINNN
                     GIVVGTFSNGQNLDLASVTLSHFNGTNYLKSLDGGAYAVTDQSGPAIIGASGSISGSS
                     LEGSNTDIASEFTKLIVTQQAYSANTKVITTANSMVQDLLNVLR"
     gene            4329..6206
                     /gene="flgK"
                     /locus_tag="VGA15_02205"
     CDS             4329..6206
                     /gene="flgK"
                     /locus_tag="VGA15_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX9586546.1"
                     /translation="MSLGSALAIAMSGLRANQSALSIVSSNIANAQTPGYISEGINQV
                     EVQTGDSGASVIVTGVNRQLDQFIQAQLRTETSGGAYADQMASVLTQLQSVYGTPGAA
                     GSLETAYSNFTTALQSLSTTSGSSSSQISAATAAQSLVQQLNATTQGIQTLRSNAEQD
                     LNISVGQANTAMSQIAQLNTRLQGLNPTDPAAATLMDQRDKAIDQLSQLMDIRVSSNG
                     NNQTSIYTTNGVELVGAQASKLSFNSQGTLNANSQWNSNPAKSSAGTITCTLANGATV
                     DMIATNSIGSGQIGADLMLRDKTLVQAQTQVDQLAASMASALSDKTTAGTAAPAALAP
                     KAGFDLDTSNVLPGNTINLTYTDAATNTQHNVTIVRVDDPTALPLSNAGGNPNNPVIG
                     VNFSGGMASVVAQLNSALGGANLQFSNPSGSTLRVVDSGTGSATINAASVTTTATSLA
                     GGSAQLPLFTDGNSLYTGVITGAGSQLTGLAGRITVNSALLTNPSNFTVYSTSPPTNA
                     GDTTRSDFLYSQLTTGTFTYSPQTGLGSAASPFKGTLTSYMQQFLSQQSNASTSATQL
                     QQGQDVVVNTLQQKMKATSGVNIDSEMANLIALQNSYAANAHVMSVVQTMMTSLMQVQ
                     L"
BASE COUNT         1219 a         2049 c         1848 g         1117 t
ORIGIN      
        1 ggacaaggtg acaagcgact gatccgtccg taagaaaccg gtatgagtga gacttcttta
       61 ggcatggccg caagatccga tggcgccgac ctgcgagagc gctcgcgcct ttcgttcgat
      121 ctggacgtcg atatctgtgt ggtaggggcg gggctcgccg gcctcaccgt ggcgctggag
      181 gccgcaaggc tcggcgccag cgtcgccgtt ctcgaaggcc ggcatgtcgg ctggaacgcg
      241 tccggccatc acctcggcac ggtgatgcca ggctttgctc ttccgatcgg cgaattgatc
      301 gcgcgcatcg gctttgaaga cagccgcgaa ctctggtcat tgtcgaaaca gggcgccgat
      361 tatgttcggg ccaacgccac cgaagagctg atgccgggca ttgcgctgag cgagggagcg
      421 ctggaggttt ccaatgtcga ggccggcgaa caattgatca gccggctgca aatgctgagc
      481 gaggatttcg agacggacgt ggaaggctgg caggtcgatc gcgtgcgcga tcagctcaag
      541 accgatcatt acttccacgg cgtgtattat cccaaggcgt ttcagatcga cggccgcaaa
      601 tacgtccacg gtcttgccgc gctgacgcgg cgggcggggg cacggatttt cgaggacacg
      661 ccggtcgtca gcatcgatcc ctcgggcatt cgcaagcgca ttgtcacgcc gtcggcgcgg
      721 ttgcgtgcgt cacacatcgt gctcgccggc aacatccatc tgggcgagcc attgcggcgg
      781 ttgtcggaga ccctgctgcc ggtctggcgt tacgccgcgg tgaccgcgcc gctcggcgag
      841 cgccttgccg acgtggtgag gtttcaggga tcggtgaccg acaccgtcgg catcgatcat
      901 ttccggatcg tggacggcga ccgcttgatg tgggccagcc cggagaccac ctgggcggcg
      961 cgaccggagc gttttgccgg ccccattgcg cgcaggatcc gcacgatctt tcccaggctc
     1021 ggaacaaccg agattgccga ggtctggggc ggtgccgtcg gcgagaccgt gcacggcatg
     1081 ccgcagatcg gccagttgcg tcgcggtctg tgggtcacca gcggcttcgg ccgtcagggc
     1141 ttgaacacgt cggcgatggc cgggcagctg atcgcgcgca gcattctgtg gggcgacgag
     1201 cgctggcggc tgttttcgcc gttcgaactg gtgtgggcgg ggggcccgac cggccgggta
     1261 gccggacatc tcgtcgggat gtgggggcgg ggcagctccg ccgccgccgg cgcgttggcc
     1321 cgctatcggg agagagcaca ggcccgggaa cgcattcgcg aggcgcgtct gctcgcggcc
     1381 aatcgccagg ccggaaccag accgccacgg cgcccgacac cgccggtccg ccctcgtccc
     1441 atgcgtcccc ggccgcctgt ccagacaaac cccggcgagg gcgagggttt cccccaccct
     1501 aaccctcccc cgcaagcggg aggggggaca tagcaccctt ggagcgggcc tctcgtatca
     1561 tcattggtgg cgatggagga atcccatatc gaatcgggag atgcgcgctc cctctcccgc
     1621 ttgcggggag ggtcggggtg ggggcatctc gcaacaaagt gagaaaattc cctcccaatc
     1681 agtgtaactt ctgcccccga gcccccgtat ctagcggcga acgataccga attcgcttcg
     1741 aacggagacc atcatgatcg accgccgtat tctgctggcg tctgtcgcca gtttgcttgg
     1801 ccttgcggcc ttccggtggc tgcgcgcttc gcctgccaac gcagcggaga agttcgagat
     1861 cgaaaagacc gatgcggaat ggcgggcgca gctgacgccg cagcaatatg agatcctgcg
     1921 caaggagggc accgagcggc cgggatcgag cccgctgctc aaggagcacc gcaagggcat
     1981 cttcgcctgt gccggctgcg acctgccgct gttctcctcg gagacgaaat tcgagagcgg
     2041 taccggctgg ccgagttttt accagccgct ggagaacgcc gttggcaaaa ccgaagaccg
     2101 gactttcggg gtgttgcgga ccgaaattca ctgccgccgt tgcggcggcc atctcggtca
     2161 cgtcttcgac gatgggccga agccgaccgg cctgcgctac tgcatggatg gctttgccct
     2221 ggtattccat ccggccgccc cgtcggcgac ctgatcgtcg cttgcgcccg gcaattgttg
     2281 ccccgctcgg caattgtgcc ccggtcatcg gaccggggct ttatttttaa ggcattgata
     2341 ttgctatagt tttcttgttg gcacaacgct tgcgaaggtc tccttcgata gcggtcatgg
     2401 cttcgcaaac caaccggccg cgcggatcga atttggagac gattgttatg ggtattttcg
     2461 acgcgctcaa taccgcggtt ggtggcctcc aggcgcaatc cttcgccttg cagaacatct
     2521 ccggcaatat cgcgaatgct tcgaccacgg gctacaaggg catcgagacc agcttcgagg
     2581 atctcattcc ggattcgacg tcgccgagca ggcaggtcgc aggcggcgtg accgcctttg
     2641 cgcaggccac gatcaccacc cagggcaccg tctcggtcac caccgtcgcg accaacatgg
     2701 cgatcaacgg cgacggtttt ttctccgtgc aaaaggccac cggaaccacc gacaaccttc
     2761 ctgtctttaa cggcgtcacc gactataccc gccgaggcga ttttcaggtc aacgccaacg
     2821 gcaatctggt caatggcgcc ggatattact tgatgggtgt gggggtcgac ccgaagaccg
     2881 gcaatccgct cggcaacgtg ccccaggtgc ttcagttcca gaacaatttc attccggccc
     2941 agagcactac ttcaatcaag tatgccgcca acctgccgtc caccccgacg acagccgtca
     3001 gctcgactgc ggcggccggc acgatcaatg ccctcggggg cctcaacccg tttgacttca
     3061 gcaacaatcc attgcctctg ggtacgccag ccacggcgcc cggcgacaac acggtgaccg
     3121 gcaccgccga gttcaaccaa ttggcaacgc cggtcgcgat taccgcggcg acgaaactgt
     3181 ccggagccgc gggaaccgat tcggtcacgc cgagcttcgc ggccggcgac accatcacga
     3241 tcgatggcgc gacctacacc ttttcggcca gccccaccaa cctcgcgacc aataccatta
     3301 acgtcggcga caccgtcggc accttgatgg gggtcatcca gaccgccacc ggcgtagcgc
     3361 cgagcatcac cggcggcgcg atcacgctgc acaccggcac ggccgccaat ctgacaatta
     3421 ctactggcgg taccgtcacg gcgaccaacg cgctcgcagc cctcggtttc tccggaggag
     3481 gcgtgacgct gtcgcgaacc cctgccggca ccgtgggcac gggaacggtc atcggcaacg
     3541 acctccagac cttcctgaac gagtcgatca gcggcggcgc cgtcaccgcc tacgattcga
     3601 ccggaacggc cgtcaatgtg cagttgcgat gggccaagac cgacagcgcg gccctcggtg
     3661 gctcgcacac caatacgtgg aacctgttct atcaaagcaa tccgaacgcc acgggctcgc
     3721 agaccgcctg ggtcaacgcc ggaaccaact tcacgttcgg accaaccgga gtgctgacga
     3781 gtccggcaac tcccgcgatc accatcccca atgtctcggt cggaaaccag tcgctcggaa
     3841 acctgtcgtt cagcatgggt gccggcactc tgacccaatt tgccagcacc ggcggcaccg
     3901 tgacgatcaa caccatcacg cagaacggct tcgccgccgg tcagcttcaa tcggtcgcca
     3961 tcaacaataa cggcatcgtc gtcggcacgt tttccaacgg gcaaaatctc gatctcgcct
     4021 cggtcacgct gtcgcacttc aacggcacca actacctcaa gagcctcgat ggcggcgcct
     4081 acgccgtcac cgaccaatcc ggaccggcaa ttatcggcgc ctcgggcagc atcagcggct
     4141 cgtcgctcga agggtcgaac accgacatcg cttccgaatt caccaagctg atcgtgaccc
     4201 agcaggccta ttccgcgaat accaaggtga tcaccacggc caacagcatg gttcaggatc
     4261 tcctgaacgt gctgcgctga tagccatgat ggcaatgggg caattcggat tgatctgagg
     4321 cggcaattat gagtttgggt tctgctctcg ccattgcgat gtccggtttg cgtgcaaacc
     4381 agtccgcgct ttcgatcgtg tcgtcgaata tcgcgaacgc gcagacaccc ggttacatct
     4441 ccgaaggcat caatcaagtc gaggtgcaaa ccggcgacag cggtgcaagc gtcatcgtca
     4501 ccggcgtcaa ccgccagctc gatcaattca tacaggctca gttgcggacc gaaacctccg
     4561 gcggcgccta tgccgatcag atggcgagcg tgctgaccca gctgcaatcg gtctacggaa
     4621 cgcccggagc tgccggttcg ctcgaaaccg cctattccaa ttttaccacc gcgcttcaat
     4681 cgctctccac gacttcgggg agctcatcgt cacagatctc ggccgcgacc gcggcgcaat
     4741 cgctggttca gcaactgaac gccacgacac agggcattca aacgctgcgt tccaacgccg
     4801 aacaggacct caacatttcg gtcggccagg ccaacaccgc aatgagccag attgcgcaac
     4861 tcaacactcg gcttcagggg ttgaacccga cagatccggc cgcggcgacg ctgatggatc
     4921 agcgcgacaa ggcgatcgac cagctgtcgc agctgatgga tatccgcgtc tccagcaacg
     4981 gcaacaacca gacatcgatc tacacgacaa acggcgttga gcttgtcggc gcccaggcat
     5041 ccaagctcag tttcaactcg caggggacgc tgaacgccaa ttcgcaatgg aacagcaatc
     5101 cggccaaatc ctccgccggc accatcacgt gcacgctcgc aaacggcgcc accgtcgaca
     5161 tgatcgcgac caactcgatc ggttccggcc agatcggggc cgatctgatg ctgcgcgaca
     5221 agaccctggt tcaggcgcag acgcaggtcg atcagctggc ggcgtcgatg gcaagcgcgc
     5281 tgtcggacaa gaccaccgcc ggcaccgcgg cccccgccgc gctcgcgccg aaggccggat
     5341 tcgatctcga tacgtcgaac gtgctgccag gcaacacgat caatctcacc tataccgacg
     5401 ccgcgaccaa tacgcagcac aacgtcacga tcgtacgtgt cgatgatccc acagcgcttc
     5461 cattgtcgaa tgccggcgga aatcccaata atccggtgat cggcgtcaat ttttcgggcg
     5521 gtatggcgtc ggtcgtagca caactgaaca gcgctctcgg cggcgccaat ttgcagtttt
     5581 ccaatccgtc cggctcgacc ttgcgcgtcg tcgacagcgg caccggctcg gcgaccatca
     5641 acgcggcttc ggtcacgacc accgccacat cgcttgcagg cgggagcgct cagctgccgt
     5701 tgttcaccga tggcaattcg ctctacaccg gcgtgatcac cggcgccgga tcgcaattga
     5761 ccggcctcgc gggacgtatc accgtcaatt cggcgctgct caccaatccg tcaaacttca
     5821 ccgtctacag cacctcgccg ccgacgaacg ccggcgacac gacgcgttcg gattttctct
     5881 actcccagct gacgaccggc accttcactt actctcccca gacagggcta ggctcggccg
     5941 cgtcgccgtt caagggaacg ctgaccagct atatgcagca gttcctgagc cagcagagca
     6001 atgcgtctac ctccgcgacc cagctccagc agggtcagga cgtcgtcgtc aatacgctgc
     6061 agcagaaaat gaaagccacc tccggcgtca acatcgatag cgagatggcg aacctgattg
     6121 cgctgcagaa ttcctatgcg gccaatgcgc atgtgatgtc tgtggttcag accatgatga
     6181 ccagcttgat gcaggtccag ttgtaaggcg atcgatatgg caatcaatag cgt
//
LOCUS       DASWGM010000067         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_420940,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000067 DASWGM010000000
VERSION     DASWGM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420940"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VGA15_02210"
     CDS             complement(<1..1154)
                     /locus_tag="VGA15_02210"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX9586547.1"
                     /translation="MFNIHSVEIDWGGRPLRLETGKIARQADGAVLATYGETVVLATV
                     VAAKTAREGVDFLPLTVDYIEKTYAAGRIPGGYFKREGRPTEKETLVSRLIDRPIRPL
                     FVDGWRNETQVIVTVLSHDMENDPDILALVAASAALTISGAPFKGPIGAARVGFINDE
                     YVLNPTLDEMVETQLDLVVAGTADAVLMVESEAKELNEDIMLGAVMFGHRHFQPVVNA
                     IIGLAEKAAKEPRDVKIVDESVLEKEILGLIETDLRTAYAIPVKQDRYAAVGKAKDKV
                     MAHYFPEGQESKQEPKYDKLRIAGVFKELEAKIVRWNILDTGKRIDGRDSKTVRQIIA
                     EVGVLPRAHGSALFTRGETQAMVVTTLGTGEDEQYIDSLSGTYKEQFLLH"
     gene            complement(1167..1406)
                     /locus_tag="VGA15_02215"
     CDS             complement(1167..1406)
                     /locus_tag="VGA15_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586548.1"
                     /translation="MPKQKSVQQQSGDWAMTGKVPVARVEGLELLPATIGRMKPSGTS
                     RPWQDRRTLIFQCDQRLAILRMVFLCRGFTPFVNP"
     gene            complement(1570..>1789)
                     /gene="rpsO"
                     /locus_tag="VGA15_02220"
     CDS             complement(1570..>1789)
                     /gene="rpsO"
                     /locus_tag="VGA15_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX9586549.1"
                     /translation="KAGDTGSPEVQVAILSERINNLTGHFKSHVKDNHSRRGLLKMVA
                     TRRSLLDYIKRKDEARYKALLEKHNIRR"
BASE COUNT          322 a          531 c          555 g          381 t
ORIGIN      
        1 tagtgcagca ggaactgctc cttgtaggtg cccgacaggg agtcgatgta ctgttcgtcc
       61 tcgccggtgc cgagcgtggt caccaccatc gcctgggttt cgccgcgggt gaacagcgcc
      121 gagccgtggg cgcgcggcag cacgccgact tcggcgatga tctggcgcac ggtcttggaa
      181 tcgcggccat cgatgcgctt gccggtatcg agaatattcc agcgaacgat cttggcttcc
      241 agttccttga acacaccggc gatgcgcagc ttgtcgtatt tcggctcctg tttcgattct
      301 tggccttccg ggaagtaatg cgccatcacc ttgtctttgg ccttgccgac cgcggcgtag
      361 cggtcctgct tgaccgggat cgcataggcg gtgcgcaggt cggtctcgat cagcccgagg
      421 atttccttct cgagcacgct ctcgtcgacg atcttgacgt cgcgcggctc cttggcggcc
      481 ttctcggcca gtccgatgat cgcgttgacc accggctgga aatgccggtg tccgaacatc
      541 acggcgccga gcatgatgtc ctcgttgagc tccttggctt ccgattccac catcagcacc
      601 gcgtcggcgg tgccggcgac caccaggtcg agctgggtct cgaccatttc gtcgagcgtg
      661 gggttgagca catattcatc gttgatgaag ccgacgcggg cagcaccgat cgggcccttg
      721 aagggggcgc cggagatggt gagcgccgcc gaggccgcga ccagcgcgag aatgtcggga
      781 tcgttctcca tgtcgtgcga cagcacggtg acgatgacct gggtctcatt gcgccagcca
      841 tcgacgaaca aggggcggat cgggcggtcg atcaaacggg aaaccagggt ttccttctcg
      901 gtgggccggc cttcacgctt gaagtagccg ccgggaatgc ggcccgcggc ataggtcttt
      961 tcgatgtagt cgacggtcag cggcaggaag tcgacgcctt cgcgggccgt cttcgccgcg
     1021 acgacggtgg cgagcaccac ggtctcgccg taggtggcga gcacggcgcc gtcggcctga
     1081 cgggcgatct ttccggtttc aagcctgagg ggacggccac cccagtcgat ttcgactgaa
     1141 tgaatattga acattgcggt cttctttcat gggttcacga aaggcgtgaa accccggcac
     1201 aaaaaaacca tgcgcaagat tgcgagacgc tgatcgcatt ggaagatcag cgtccggcga
     1261 tcctgccatg gtcttgatgt gccggatggt ttcatccttc cgatagttgc cggcaaaagc
     1321 tctaagcctt cgacccgcgc gacgggcacc ttgcccgtca tcgcccagtc gccggattgc
     1381 tgctggacgc tcttctgttt gggcatgatg ctttccgaaa accggattcc acttttcggg
     1441 atcatgccgt ctgtgcaggc agacgaaatg cgcctgcacc tggaaatcgc atgatctgat
     1501 cgaaaaaccg aaaaacgctt ttccggacca tgcgcaaaaa cgcgcgcgag atgtacgcgc
     1561 gaaccgtact caacgacgaa tgttatgctt ttcgagcagc gccttgtaac gcgcctcgtc
     1621 cttcctcttg atgtaatcga gcagcgaacg gcgtgtggcg accattttca aaagcccgcg
     1681 acgcgaatga ttgtccttca cgtgggattt gaaatgcccg gtgaggttgt tgatgcgttc
     1741 cgacaggatc gcgacctgaa cctcgggcga gccggtgtcg ccggccttg
//
LOCUS       DASWGM010000068         6160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1251733,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000068 DASWGM010000000
VERSION     DASWGM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251733"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VGA15_02225"
     CDS             <1..455
                     /locus_tag="VGA15_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9586550.1"
                     /translation="AQPIVFVVHDWGSALGFDWANHHRDRVRGIAYMEGIVRPVAGWE
                     EWSEAATPIFQGFRSDKGETMILDRNMFVERVLPGSVLRKLTEAEMTEYRRPFLQARD
                     RWPTLTWPRQIPISGEPADVVQIATDYSKWMAENDIPKLFVNADPARF"
     gene            719..1123
                     /locus_tag="VGA15_02230"
     CDS             719..1123
                     /locus_tag="VGA15_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586551.1"
                     /translation="MFSSRDAYENDFCPVRDELLGEMYRANENGLAQLVESVSFDVRA
                     MLALFCYRRSHLHSLALSIAATCSERELIHLGGRVGSTLYALSREAPATRSAPSSHGG
                     RKPITLSTRPLSTFSPAEDEFDGEDLLQAATA"
     gene            complement(1268..1588)
                     /locus_tag="VGA15_02235"
     CDS             complement(1268..1588)
                     /locus_tag="VGA15_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX9586552.1"
                     /translation="METSNPAIDVESPCQACGACCSYSSNWPRFTTEDDAALDLIPEK
                     FVNEKLSGMRCDGERCSALSGKVGVATSCVIYAVRPEVCRTCMPGDAECAMARRRHGM
                     PVLT"
     gene            complement(1720..2418)
                     /locus_tag="VGA15_02240"
     CDS             complement(1720..2418)
                     /locus_tag="VGA15_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX9586553.1"
                     /translation="MRRADRLFQIIQVLRRTRKPLTADAIAAELETSKRTIYRDIATL
                     MAQRVPIRGEAGMGYILEKGFDLPPLMLTPDEIEAAVLGAQWVAGHADSVLARAAEDL
                     MAKIADTVPERLRPFVLEPASRARPGWRREPDRIDMARTRAQIHEGKKIALRYRDEHG
                     RDSERTIWPIAVGYLEAVRLLAAWCELRRDFRSFRTDRVIDAVHLDEKYPERRDLLRA
                     RWRRSLVWEPPSDT"
     gene            2521..2793
                     /locus_tag="VGA15_02245"
     CDS             2521..2793
                     /locus_tag="VGA15_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586554.1"
                     /translation="MMILYGFGGFRPFWRAQVWAVEHLISVNCASGDFRWAIAALVLR
                     WFGVLEAFAQKQLASLARTVMGRAWQGARFAAHMTDADTMVATGGS"
     gene            2829..3557
                     /locus_tag="VGA15_02250"
     CDS             2829..3557
                     /locus_tag="VGA15_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9586555.1"
                     /translation="MITLYGFGPGFGLPEISPFVTKTEVQLKMAGLAYRKEKAMPPAS
                     PKGQLPYIADEAERIADSTFIRAHIEGKYAFDFDAPLNLQQRAQAWAFERMIEHHVYW
                     ALVGARWVDAENFAKGPAHFFDAAPEHLREKLREDAQFRVAENYLLSGLGRHAPEEDV
                     DLAVRSLFALSVQLGDKPYLMGDTPCGTDATAFGALAGILTPFFASQLRERAEHFQNL
                     TAYVDRMMLRYYPDFAWAPLRQAA"
     gene            complement(3554..4189)
                     /locus_tag="VGA15_02255"
     CDS             complement(3554..4189)
                     /locus_tag="VGA15_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9586556.1"
                     /translation="MSDVHSYSSDVAFTPAVKEIQARKGSREAYAHVERNGGWRTEID
                     DNLAAFLTDANSLYFATASADGQPYIQHRGGPKGFVRILDKNTLAFADYSGNRQYITQ
                     GNLSENPKAHIFLMDYAHRRRVKIWGEALVVNDDPALTKSLMPQGYKARPEQVILFKI
                     AAWDTNCPQHIPQKFDAADVAAALASRDAKIAELEAELAALKGAPARDIKA"
     gene            4317..5216
                     /locus_tag="VGA15_02260"
     CDS             4317..5216
                     /locus_tag="VGA15_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9586557.1"
                     /translation="MDRIDAMQAFVAVADLHGFAPAARKLGLSPSGVTRLIAALEDRL
                     GARLLQRTTRSVTLTDIGTRYLERARRILADVEEAEGSAEGERTRPSGRLVVSAPVGF
                     GRLHVSPVMSAYLKRYLEVLGELRLADRTVNLVEDGVDLAVRIGHLPDSSLVARHVGE
                     MRRIVVASSDYLRQRGEPDTPEAIASHETIQFGAMTASPDWRFVEDGREIRVACTPRF
                     TTNSADAAIQYAEQGGGLTRVLAYQAAEAITAGRLKIVLAKFEQPPLPIHIVYPTSRL
                     LSAKVRTFIDLVIELSDWHFGKR"
     gene            complement(5368..5787)
                     /locus_tag="VGA15_02265"
     CDS             complement(5368..5787)
                     /locus_tag="VGA15_02265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9586558.1"
                     /translation="MIATDVPEGFERQTRPSPLTEPWEPIYSKLTPDAIILGLRLAKP
                     HTNARGFAHGGLIAALTDKAMGHSCGHKMRGAYSLVTVSMSIDFISSAQIGQWLTIET
                     DVIKTGSTLCFAQCLVTADGIAIARANGTFRVVQKKG"
     gene            complement(5965..>6160)
                     /locus_tag="VGA15_02270"
     CDS             complement(5965..>6160)
                     /locus_tag="VGA15_02270"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX9586559.1"
                     /translation="GWALGHYSIADIHLFRLYWRLANSLKPAPETFPHLTVHYARMMA
                     RPAVQRTIEVETAIGYQLPA"
BASE COUNT         1093 a         1879 c         1955 g         1233 t
ORIGIN      
        1 ccgcgcaacc catcgtgttc gtggtgcacg actggggctc ggcgctcggc ttcgactggg
       61 ccaaccatca ccgcgatcgc gtccgcggca tcgcctatat ggaggggatc gtgcggccgg
      121 tcgccggctg ggaggaatgg agcgaggcgg cgacgccgat cttccagggg tttcgctccg
      181 acaagggcga aacaatgatc ctcgaccgca acatgttcgt cgagcgggtg ctgccaggct
      241 cggtcttgag gaaactgacc gaggccgaga tgacggagta tcgccggccg tttctgcaag
      301 ccagggaccg ctggccgacg ctgacctggc cgcggcagat tccgatatcg ggcgaacccg
      361 ccgatgttgt gcagatcgcg acggactatt cgaagtggat ggccgagaac gacattccga
      421 aactgtttgt caatgctgat ccggcgcgat tctgatcggg gctgtccggg atttttgccg
      481 aagctggaaa agccagaccg aagtcaccgt gccaggctcg catttcatcc aggaagattc
      541 cgggccggcg atcggccggg cggtggcggg gtggatgaag gcaaactcgc tatagcggga
      601 cgggggtggc gccgtcccga ttcgccgcgg tcaagacacc ccgtgtcact accgggtagg
      661 gtggttaacc tttcgttaga gggttacgct gcatcgtgcg gacaggggag accgcaaaat
      721 gttctcaagc cgcgatgcct acgaaaacga tttctgtccg gtccgggacg agttgctcgg
      781 tgaaatgtac cgcgccaacg agaacggcct ggcgcagctg gtggagagcg tctcgttcga
      841 tgtccgggcg atgctggcgc tattctgcta tcgcagaagc cacctgcact cgctggcgct
      901 ctcgatcgcc gcaacctgca gcgaacgcga attgattcac ttgggcgggc gcgtcggctc
      961 cacgctttac gcgctctccc gcgaagcgcc ggcgacgcgt tccgcgccgt cgtcccacgg
     1021 cggccgcaag ccgatcacgc tctcgacccg accgctcagc accttctcgc cggccgaaga
     1081 cgagtttgac ggcgaagatt tactgcaagc cgcgacggcg tgatcgatca cgtaatttct
     1141 ccgcgcgtca tcccggcgaa cgccgggatc cataaccacc gacatcaatt gttacggaag
     1201 gcatttgcct cggtgcctga acgatagtgc acggagtata ggtcccggcc ttcgccggga
     1261 cgactcgtca ggtcagcacc ggcatgccgt gccgtctccg cgccatcgcg cattcggcat
     1321 cgccgggcat gcaggtccgg cagacttcgg gccgcacggc ataaatcacg cacgacgtcg
     1381 ccacgccgac cttgcctgac agtgccgagc atcgctcgcc gtcgcagcgc attcccgaca
     1441 gtttttcatt gacgaatttt tccggaatca gatcgagcgc cgcgtcgtcc tcggtggtga
     1501 agcgcggcca gttcgaggaa tacgaacagc aggcgccgca ggcctggcac gggctttcga
     1561 cgtcgatagc cggattgctt gtctccatgc cttccgttta gagcatgatt cggaaaactg
     1621 gaaaccggct ttcaggacag gcgccaagcg cttgcccgaa agatcatgct caaaccattg
     1681 agatcatgga gcgatcggcc gattggccgc accagcgcgt caagtgtcgc tcggaggttc
     1741 ccagactagg ctccttcgcc atcttgctct cagcaagtca cgccgctcgg ggtatttttc
     1801 gtccagatgc acggcgtcga tgacacggtc ggttcgaaag ctgcgaaaat ccctgcgcag
     1861 ctcgcaccat gccgccagca gccgcacggc ctcgagatag ccgacggcga tcggccagat
     1921 ggtgcgttcg ctgtcgcggc cgtgttcgtc gcgatagcgc agcgcgatct tcttgccttc
     1981 gtgaatctgc gcccgcgttc gcgccatgtc gatgcggtcg ggttccctgc gccagcccgg
     2041 gcgggcgcgg ctggcgggtt caagcacaaa aggcctcagg cgttccggga ccgtgtcggc
     2101 gatctttgcc atcaggtctt cggcggctct tgccagcacg gaatcggcgt gacccgcgac
     2161 ccattgcgcg ccgagcacgg ccgcttcgat ctcatcgggg gtgagcatca gcggcggcag
     2221 gtcaaacccc ttctccagga tatagcccat cccggcctcg ccgcggatcg gtacccgttg
     2281 cgccatcagg gtggcgatgt cgcggtagat cgttcgcttc gaggtctcta gctcggctgc
     2341 gatcgcatcc gccgtcagtg gcttccgggt gcgccggagc acctgaatga tctggaacag
     2401 ccggtcggcg cgtctcatgg ggcgtctctt tttacagggc agcctcgccg atgctgacag
     2461 catgttggca gcaggcggtg gctataccgg gacaacactt caactgaagg gaacttgtcc
     2521 atgatgattc tgtatggctt cggaggtttc cggccgttct ggcgtgccca ggtttgggcc
     2581 gtggagcacc tgatttccgt aaactgcgcc tccggggatt tccgctgggc gatcgctgca
     2641 ctggtcttgc gttggttcgg cgtattggag gctttcgcgc agaagcaact ggcctccttg
     2701 gcccgaaccg tcatgggtcg cgcctggcag ggggctcgtt tcgcggccca tatgaccgat
     2761 gctgacacta tggtggcaac agggggtagc tagggtcgcg catcatttca agagatcgga
     2821 gccctcacat gattactctt tatggctttg gccccggctt cggcttgccg gaaatcagcc
     2881 cgtttgtgac caagaccgag gtccagctga aaatggccgg tcttgcctat cgcaaggaga
     2941 aagcgatgcc accggcttct cccaaggggc agctgcctta tatcgcggat gaggcagagc
     3001 gcatcgccga ttccaccttc attcgcgcgc atattgaagg caaatacgct ttcgatttcg
     3061 acgcgccgct caatctgcag cagcgcgctc aggcctgggc gttcgagcgg atgatcgagc
     3121 accacgtcta ttgggcgctg gtcggtgcgc gctgggtcga cgccgaaaac ttcgccaaag
     3181 ggccggcgca tttcttcgat gcggcgcccg agcatctgcg tgaaaaactt cgcgaagacg
     3241 cgcaattccg tgtcgccgag aactacctgc tgagcggtct cggccgccac gccccggaag
     3301 aggatgtcga tctcgcggtc cgctcgttgt ttgcgctgtc ggtccagctc ggggacaagc
     3361 cctatttgat gggcgacacg ccgtgcggca ccgatgccac cgcgttcggg gcgcttgccg
     3421 gaattctgac gccgttcttc gcctcgcaac tgcgagagcg ggccgagcat ttccagaatc
     3481 tcacggccta tgtcgatcgg atgatgcttc gatattatcc ggactttgcc tgggcaccgc
     3541 tgcggcaggc ggcctaagcc ttaatgtccc gcgctggcgc gcctttcaac gccgccagct
     3601 ccgcctcaag ttccgcgatc ttggcatcgc gcgaggccag cgccgccgcc acatcggcgg
     3661 cgtcgaactt ttgcgggata tgctgcgggc agttggtgtc ccacgctgca atcttgaaca
     3721 ggatcacctg ttcaggccgc gccttgtagc cttgcggcat cagcgatttt gtcagtgccg
     3781 gatcgtcatt caccaccagc gcctcgcccc atatcttgac gcggcggcgg tgggcgtaat
     3841 ccatcaggaa gatatgggcc ttcgggtttt ccgaaaggtt tccctgggtg atgtattgcc
     3901 ggttgccgct gtaatcggcg aacgccaggg tattcttgtc cagaatcctg acaaaacctt
     3961 ttgggccgcc gcggtgctgg atatagggct ggccgtcggc gctggcggta gcgaaataca
     4021 ggctgttcgc gtcggtcaga aacgcggcca ggttgtcgtc gatctcggtg cgccatccac
     4081 cgttccgttc gacgtgcgca taggcttcac gcgatccctt gcgggcctga atttccttca
     4141 ccgccggggt gaacgcgaca tcgctcgaat agctgtgaac gtctgacatc ggggatctcc
     4201 ggaaaaactc gtccgggcga tgcagcgccc gtcttcgcta cccaacatgg ctcctttcga
     4261 aatttaagca atcttatgaa ttgacacttc atcattgcaa tatatgaaat aatgccatgg
     4321 atcgcatcga tgccatgcag gcctttgtcg cggtggccga cctccacgga tttgcgccgg
     4381 cggcccgcaa actcggcctg tcgccatccg gcgtgacgcg gctgattgct gcgctggagg
     4441 atcgtctcgg cgcgcggcta ttgcagcgga ccacgcgctc ggtgaccctg accgacatcg
     4501 gcacgcgcta tctggagcgc gcacgtcgca ttctggccga tgtcgaggaa gccgaaggct
     4561 cggccgaggg cgaacggaca cggccgagcg gacggctggt ggtctcggcg cccgtcgggt
     4621 tcggacgcct ccacgtcagc ccggtgatgt cggcgtatct caaacgctat ctcgaggtgt
     4681 tgggcgaact gcggctggcg gatcgcacgg ttaacctggt ggaagacggc gtcgatctcg
     4741 cggtcaggat tggccacctc ccggattcca gcctggtcgc ccgccatgtc ggcgagatgc
     4801 ggcgaatcgt agtcgcctcc tcagactatc tcaggcagcg cggcgagccg gacacgcccg
     4861 aggcgatcgc ttcgcatgag accatccagt tcggtgccat gaccgcgtcg ccggactggc
     4921 gctttgtcga agacggccgt gaaattcggg tcgcgtgcac gccgcgcttc accaccaaca
     4981 gcgccgatgc cgcgatccag tatgccgagc agggcggcgg cctgacccgg gtgctggcct
     5041 atcaggcggc ggaggcgatc acagccggcc ggctgaaaat agtgctggcg aaattcgagc
     5101 agccgccgct gccgatccac atcgtctatc cgacctcgcg gctgttatcg gccaaggtgc
     5161 gcaccttcat cgatctcgtg atcgaactta gcgattggca tttcggcaaa cggtgaggcg
     5221 ttccccggat gctgcgcagc gcgctagcgg tgcgctgctg gtccggggtc catatgtcgc
     5281 tataggtgtg tcgctatggg tcccggctct gcggagcggg gtaaaggacg ccgcaccgcg
     5341 tccgggacac gaaatagcgt gcggacgcta ccccttcttc tgcaccaccc gaaatgtccc
     5401 gttggcgcgc gcgatggcga taccgtcggc ggtaacgagg cattgcgcaa agcaaagcgt
     5461 gctgccggtc ttgatcacgt cggtttcgat cgtgagccac tgcccgatct gggcgctgct
     5521 gatgaaatca atcgacatgc tcacggtcac gagcgaatag gcgccgcgca tcttatggcc
     5581 gcagctatgc cccatcgcct tgtcggtcag cgccgcgatc aacccgccat gggcaaagcc
     5641 gcgcgcattg gtgtgcggct tcgcaagacg aaggcccaga atgatcgcat cgggtgtcag
     5701 cttcgagtag atcggctccc acggttcggt cagcggactg ggccgggtct ggcgctcaaa
     5761 accttcgggg acatcggtag caatcatgtg atgcgctcgc gcgttagcaa tacagtttgc
     5821 attgaacgcg atcgcgaggt gaaatgcacc gcatacaaag ttttaaacgg gattttcggt
     5881 gtttgtttga agctgatgcg gcagtcagct ggttgtcatc atccacgaaa gcgctttcgc
     5941 ggggtatgac ggtcaggacc ccaatcacgc cggcaattgg tatccgatcg cggtttcgac
     6001 ctcgatcgtg cgttgtacag cggggcgggc catcatgcgc gcataatgca cggtgagatg
     6061 ggggaaggtt tcgggcgcgg gcttgagcga attggcgagc cgccaataga ggcgaaaaag
     6121 atggatgtcg gcgatcgagt agtggccaag cgcccaaccg
//
LOCUS       DASWGM010000069         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2222425,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000069 DASWGM010000000
VERSION     DASWGM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222425"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..995
                     /locus_tag="VGA15_02275"
     CDS             267..995
                     /locus_tag="VGA15_02275"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9586560.1"
                     /translation="MQRSRICKGCWQQMHLPVPLRGMLSVPFRAFGIRPSKMNPNTCT
                     ICELMFTRVMKARKVSVDATVLFADLRGYTGLSQSLSPDAISGLLDTFYDECANAIWA
                     YDGLLNKTIGDAVMAIFNFPIRGEDHANRAVRAAREIQQRWASERENLAKTLGVDGSE
                     LGVGIGIHSGELSFGEFGRSHRDLTAIGTVVNTASRAQTVADADQILVTEAVYQRARS
                     DLSGSQSKAYRLKGFEAPIELYAA"
     gene            complement(1022..2047)
                     /locus_tag="VGA15_02280"
     CDS             complement(1022..2047)
                     /locus_tag="VGA15_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9586561.1"
                     /translation="MSQSAKRVVLVSRPVGEPKASDFRVEEYALPTPGDGQVLLRTIW
                     LSLDPYMRGRMSDGPSYAAPVPIGGVMEGGTVCEVIASNNPGFAKGDIVLSRAGWQTH
                     ALSDGKDLRKVDPSLGPVSSAVGVLGMPGMTAYTGLLDIGRPQAGETVVVAAASGAVG
                     SAVGQIAKIKGARAVGIAGGKDKCDYVKKELGFDDCLDHRDPDLAAKLKQACPKGIDV
                     YFENVGGKVFEAVFPLLNAFARVPVCGLIAQYNDTEASVPKWASSMMRAILTKRLNIR
                     GFIVSDFAARHADFLRDMSGWVREGKVKHREFVTEGLDSAPAAFMGLLKGANFGKQLV
                     RVGPDRA"
     gene            complement(2348..>2669)
                     /locus_tag="VGA15_02285"
     CDS             complement(2348..>2669)
                     /locus_tag="VGA15_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586562.1"
                     /translation="KLGGMQRDGSVAVDRYISYRRGKDIVSLSVFLLKDQPVESHEFH
                     VIAPKALLDGAKPVAEIRPTATEAQKQSADGGLAFKDLPSAAAAFDAALAKITARTPH
                     PIHE"
BASE COUNT          525 a          832 c          782 g          530 t
ORIGIN      
        1 ggaggagaag accggtgaaa tgcgtttgca gatatcggcc tcagcatcgc aattgaatgc
       61 gacattctcg gttgccggcg ttgaatgcac ctacagcggc cgattgacag actcttatgc
      121 aggcacgatg gcctgccccg atcgacaggc cgttcccctc aagctatggg tacgatagtt
      181 gcctgccaag acacgctccc tgtcgacgtc gcttgtgggc cgccattgcc agcgacgcca
      241 ttgaagtggg ttggtgcctg atgactctgc aacggtcgag aatctgcaag ggatgctggc
      301 agcagatgca tctgcctgtg ccattgcgcg ggatgctctc ggtgccattt cgagcgttcg
      361 gcattcgccc gagcaagatg aacccgaata cctgcaccat ctgcgagttg atgttcacga
      421 gggtgatgaa ggctcgaaag gtctcagtcg atgccactgt cctgttcgcc gatctgaggg
      481 gctacaccgg cctgtcgcag tccctgtcgc cggacgctat ttcaggactg ctcgacacgt
      541 tctacgatga atgcgcgaac gccatttggg cgtatgacgg gttgctcaac aagaccattg
      601 gagacgcggt catggctatc ttcaacttcc ccatcagggg agaagatcat gccaatcgtg
      661 cggtacgcgc cgcgcgcgag atccagcaac ggtgggcgtc cgagcgcgaa aacctcgcca
      721 agacgcttgg cgtcgatggc agcgaattag gggtaggcat tggtattcat tccggtgaac
      781 tgagctttgg cgaattcggc cgctcgcatc gcgacctgac cgcaatcggc acggttgtaa
      841 acaccgcttc gcgggcgcaa acggtcgccg atgccgacca gatccttgtc acagaggcgg
      901 tctatcagcg cgcgcgatcg gacttgtcgg gcagccagtc aaaagcgtat cgccttaagg
      961 gctttgaggc gccgatcgaa ctttatgcgg catagcgcca gatcgatcaa cgtcgggcac
     1021 ctcacgctct gtccggcccg acgcgtacca gctgcttgcc gaaattggcg cccttcagca
     1081 gtcccatgaa tgcagcgggc gcgctgtcca aaccctccgt gacgaattcg cgatgcttga
     1141 ccttgccctc gcgcacccac cccgacatgt cgcggagaaa atccgcatgc cgcgcggcga
     1201 aatcgctgac gataaagcct ctgatattca gccgttttgt caatatcgcg cgcatcatcg
     1261 acgaggccca tttcggcacc gatgcttccg tgtcgttata ctgcgcgatc aggccgcaca
     1321 ccggcacacg cgcgaacgca ttgagcagcg ggaataccgc ttcgaacacc ttacctccca
     1381 cattctcgaa atagacgtcg atgcctttcg ggcaggcttg cttgagcttg gccgcgagat
     1441 cgggatcgcg atgatcgagg cagtcatcga agccgagctc ttttttcaca taatcgcatt
     1501 tgtccttgcc gcccgcgatg ccgacggcac gcgcgccctt gatcttcgca atttgcccga
     1561 ccgccgagcc gaccgcgccg gatgcggccg caacgacgac ggtttcgccg gcctgcggcc
     1621 tgccgatgtc gagcagccca gtataggcgg tcatgccggg catgccgagc acgcccaccg
     1681 cgctcgagac cggaccgagg ctcggatcga ccttgcgcaa atccttgcca tcggaaagcg
     1741 cgtgcgtttg ccagccggcc cgtgacagca cgatatcgcc cttggcaaag cccggattgt
     1801 tcgaggcgat cacctcgcac accgtgccgc cctccatcac cccgccgatc ggcaccggcg
     1861 ccgcatagga cggcccgtcg ctcatccgcc cgcgcatgta gggatcgagc gacagccaga
     1921 tggtgcgcag caggacctgg ccatcacccg gtgtcggcaa cgcgtactcc tcgacgcgga
     1981 agtccgacgc cttgggttcg ccgacgggac gagaaaccag aacgacacgt tttgcggatt
     2041 gcgacatggt tgtttcctcc aatttttttt gaacgacaaa ctagcggtag cggaagtgcg
     2101 ccccctctcc catccaactc gggtttaccc gagttggaca tctcaaatcg tcgaagtcgg
     2161 ataaatccga cttcgatggg agagggttgg ggtgaggggt tacggcctaa cgttatgctg
     2221 taatccctca cccggcgcta cgcgatagcc aatgcgaagc attggcgttc tattccaagg
     2281 acggcggccg taggccgcct atgctctccc gatgggagag gtgaccaaac tcgcacattc
     2341 cgtttcttta ttcatggatc ggatgcggcg ttcgagccgt gatcttcgcc agcgcagcat
     2401 caaacgccgc ggcggcgctt ggcaaatcct tgaaggcaag cccgccgtcg gccgactgct
     2461 tctgcgcttc cgtcgcggtg ggacgaatct cggccaccgg cttggcgcca tcgagcaggg
     2521 cttttggtgc gatcacatga aactcatggg attcgaccgg ctgatccttc agcaggaaga
     2581 cactcagcga gacgatgtcc ttgccgcgcc ggtacgaaat atagcggtcg accgcgaccg
     2641 agccgtcgcg ctgcatgccg cccaatttg
//
LOCUS       DASWGM010000070         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1668131,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000070 DASWGM010000000
VERSION     DASWGM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668131"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..670)
                     /locus_tag="VGA15_02290"
     CDS             complement(17..670)
                     /locus_tag="VGA15_02290"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX9586563.1"
                     /translation="MVTNDRSVQFAGGVLRRHRHICAFFNSTDEEHRVLRSFIKDGFE
                     RGDKAFHLVNPELREDHLKRLAEAGINVQEGMDTGQLEVRRWQDAYLRGDHFDQNAML
                     ALIEEVLQSADTAGYPLTRLLAHMEWALLDKPGVDNLVEYETRLNYVLPKYDDIVICA
                     YDLSKFGASVVMDALRTHPAVIIGGILQENPFFVPPDQFLLELRERRSVRKSAGMAN"
     gene            complement(1352..1678)
                     /locus_tag="VGA15_02295"
                     /pseudo
     CDS             complement(1352..1678)
                     /locus_tag="VGA15_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
     gene            complement(2098..2385)
                     /locus_tag="VGA15_02300"
     CDS             complement(2098..2385)
                     /locus_tag="VGA15_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586564.1"
                     /translation="MSNNFRLTTAATLGLLLASVAAPIALADVPGYEFQDFDQNLSVT
                     DSGGAARAATDAQIAAANRKADQALATAQEAIREAQQAKQQPMHQAMQRQR"
     gene            complement(2988..3227)
                     /locus_tag="VGA15_02305"
                     /pseudo
     CDS             complement(2988..3227)
                     /locus_tag="VGA15_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018484343.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3308..3583)
                     /locus_tag="VGA15_02310"
     CDS             complement(3308..3583)
                     /locus_tag="VGA15_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586565.1"
                     /translation="MQTHNHAIHLARAAAFAIALIVPSVSTLLTTAYADNESAGAIAP
                     PYQAAGNDKARSTLCGPAVSAKSDVLDAGGQRDELARKIYRPGVSDR"
     gene            complement(4034..>4418)
                     /locus_tag="VGA15_02315"
     CDS             complement(4034..>4418)
                     /locus_tag="VGA15_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9586566.1"
                     /translation="HARDGEVMLAYAMNGEQLPLLNGFPLRLVVPGWYSTYWVKMLND
                     IEVLDQPDTNFWTKTAYTIPDTPYASIYPGETGVKMVPINRMVPRSFITNIKPGDPFH
                     AGRACHSAGMVLNQPALSRRRGKPK"
BASE COUNT          808 a         1389 c         1359 g          862 t
ORIGIN      
        1 gcgattctgt gggcatttag ttcgccatgc cggcactttt gcgaaccgac cgtcgctccc
       61 gcaactcgag gagaaactga tcgggaggaa cgaagaacgg gttctcctgc aggatgccgc
      121 cgataatcac ggcgggatgc gtccgcaagg cgtccatcac gacgctcgcg ccgaatttcg
      181 acagatcata cgcgcagatc actatatcgt cgtacttggg aagcacgtag ttgaggcgtg
      241 tctcgtattc cacgaggtta tcgacgccgg gtttgtcgag cagggcccat tccatgtgcg
      301 ccaacagtct ggtgagcggg tacccggcgg tgtcggccga ttggagcacc tcttcgatca
      361 aggccaacat cgcattctgg tcgaagtggt ccccgcgcag gtaagcgtcc tgccaacgcc
      421 gcacctccaa ttggccggtg tccatcccct cttgcacatt gatacctgct tcggcgagcc
      481 gcttgagatg gtcttcccgt agctcggggt tgacgagatg gaaagcctta tccccccgct
      541 cgaagccatc tttgatgaac gagcgaagca ccctgtgctc ctcgtcggtg ctgttgaaga
      601 aagcgcagat gtgacggtgc cggcggagca cacccccggc aaactgcacg gagcggtcat
      661 tggttaccat atgtcacctc tcagacagcg agagttaggg ccgatctaga agcgccaacc
      721 aggtgccatt gcgacaatca gaactgcaac gccgcgacgc agaaccaagg tagggacgtc
      781 ggcgatggag cgtggccact gcgccatggc ttgcctcctg gatcgtggca ggcgcggttt
      841 tcgcaggcag ctttggggaa cacggtaaca cacacccctc agttctgcga gcccggtcgg
      901 caggtgggac ggcggcccag cgtctgcgtc gccgcgagat cacgggctgc ccgggtggag
      961 catgtccgac gtcgacgtcc ggcggcgatc aatgcccggc gggccgggaa gccgtacttt
     1021 cacgatggcc ggttggacga tgctccgacg cgaacagcag acttattccg taatcagagg
     1081 tagcgcacct tatcgtcagt cattcagtca gtttccatga cgggtgacaa tgcgacggca
     1141 ccgggcgcgt ccggctcatc gtcggcggtg cgctggggct tcgaagaggt gcagtgaggg
     1201 atacgccagt cctagacggc cccgcagagc cagctttgga agcagggggc cggttcttgc
     1261 cggcgcccaa tcagccgctc gatccgttac gcgtggtggg atccaatcac gcgtcagccc
     1321 gtgtcccgcg tctgcaaagg cggctcagca ctcaaggtgt ctttgtcggc acggccaacg
     1381 acatcgccgc cgtctcggag attgccgtgt cggtctcctt gctcaacccg gccacgccca
     1441 cggcgcccac gacctggccg tccagcaaga ccggcatgcc cccgcgcaga gccgcgatgc
     1501 cggcggtgac gaatgccgtg cgaccctggt tgatgttgtc ctcgatctcc gctgtcgacc
     1561 gccgcaggcg cgccgcggtc cgcgccttct ggatggcgag atcgacactg tccgggcgga
     1621 caccatccat ccgctgaaag gccagcagat cccccgccgg atcgaccacc gcaacgccga
     1681 cggcgcgctc ttctgctggc cgctctcctc ggcggctttg agcacgattt gcgctccggc
     1741 ctcattcaac accgtcacct tgacggtctg cgcctccgcc gacaggcaga aggccgacag
     1801 gatgacggcg atcgcgatcg cacggcgcat tacctcttgt ccctcagggg ggcaccctca
     1861 cgcgtccgcg ctccgaccgc ggcggtggct catgccgcag cgatccgtct gggcgaagcg
     1921 tgaacggcca ctccttggtg ctggggtcaa ccggcccatg tcggcgtctg tccgcgagtg
     1981 tccgaacgat cggaaccgga agctgagctt gcctgatctt gtggtggaaa cggtcgatgt
     2041 tcatggtctc gaccgttccc accctcggca ctggaaggca gcagcgacgc aagaatgtca
     2101 ccgctgtcgc tgcatcgcct ggtgcatagg ctgctgcttg gcctgctgcg cctcccggat
     2161 agcctcctgc gccgtcgcga gcgcttgatc ggctttccgg ttggccgctg cgatctgcgc
     2221 atcggtcgcg gcgcgagcgg caccgccaga gtcggtcaca gacaggttct ggtcgaaatc
     2281 ctggaactca tatcccggga catcggcaag ggcaatcggc gcggcgacag acgcgagtaa
     2341 gagacctagt gttgcggcgg ttgttaggcg gaaattgttc gacattgcgg ttctccttta
     2401 acgaggcgct gctgccgcgc tctcgacgac tggaacgtat gaccggcacg ctcatcggag
     2461 aacctatgcc cggcgcgggc atttgataac ggcgcgtccg tgaataaacc gttcggcgat
     2521 gctgcaatca caagggcgtg gacaaacgca cgctggcgcc ggtggtctat gaaccgcgtc
     2581 ccggcacgga ctcactttct taccaggttt tcgtcgtccg cgatgtcgat ccaaagcgac
     2641 catgaatcga tcagggccgt aagcaagtcg aacgcgattc cgcgaaatct cggcgcatcc
     2701 atgttcagag ccccagagct ttgtttgcag gcggcatcaa ccgcccgttt tcgtattgcc
     2761 atcagctacg atcgatgtcc cgcaagacat gccgcatacc ccgcggatgt tgccgaacaa
     2821 ggtaccagcc gtggctcgcc caccagcgac tggccgatca acgcgttcga ttccggcgtt
     2881 tcacatcggc tgaatagccc cctcacgcgg actatggctg gacaacttca cgatctcgac
     2941 gacttccgat ccgacttggt cggtccaaag gtcttctgca atccgctctc ggccgcctcg
     3001 agctgggcca ggatgagcgt acatttctcg aaatacgatc ggccggcctc ggtgaggctg
     3061 agcgtgcgcg tggtgcggtt caagagccgc gcgccgagcc ttccctcgag cgcgtgaaca
     3121 tgattggtca ccatggccgg cgagatgttg agacgcctcg ccgcagcggc gaagctgccg
     3181 ctggtgacga cggtggcgaa ggctgtcatg ctggtaatac gatccatcga tcacacctta
     3241 cccagtaccg cgattaccga gaccgccggc atgtgcgtca tcagctcgtt gcagtggcgc
     3301 ccgcgtctta ccgatcgctc acgccgggcc gatagatctt gcgggcgagt tcgtcccgtt
     3361 ggccgccggc gtcgaggacg tcgctcttcg cgctcaccgc gggcccgcaa agagttgacc
     3421 gcgccttgtc gttcccggcg gcttggtaag ggggcgcgat cgctccagcg ctctcgttgt
     3481 cggcataggc cgttgtaaga agcgtcgaga cgctcggaac gatcagcgct atcgcgaacg
     3541 cggcagcacg tgccaaatga atagcgtggt tgtgcgtctg cattgtgatc tccgttcgac
     3601 ctgggcagcc cgaaaagcca agccgcgact ggcgatcacg tgtggatact gccctcagtt
     3661 tttaaccggc cgatggtgaa aagatcattc atcggagttg atcaatcggc ctccgccgaa
     3721 ggctgtggca gtgtcgcatt ccctgaccca aaaacggtat aaatgcccca atacaaggcc
     3781 aaacggtcca cttcgttatc gccgtcgaga aacagcttga actgcgccga gcccagctac
     3841 ccacttcttc aatcagctcg cgggctgtcg cggctagtcc ttcagctgaa ccctgggccc
     3901 aatattgggc gcggtcagcg ctgctcggac gagtcgccga cctatgacgc gcccttgatg
     3961 ctcataagat aattgacgat cgcgttcaca tccttgggat cgatcggagc tttgtaggcg
     4021 tcgcgcatct tgttcacttc ggcttcccac gccgtctgga cagcgccggc tgattgagaa
     4081 ccattccggc ggaatggcag gcgcgcccgg catggaaggg gtcgcccggt ttgatgttcg
     4141 tgatgaaaga gcgcggtacc atccggttga tgggcaccat tttcacgccg gtttcgccgg
     4201 gatagatgct ggcatagggc gtatcgggaa tcgtgtaggc cgtcttcgtc caaaagttcg
     4261 tgtccggctg gtccagcacc tcgatgtcat tgagcatctt cacccagtag gtcgaatacc
     4321 agccgggaac gacgagcctg agcgggaacc cgttgagcag cggcagctgc tcgccgttca
     4381 tggcgtaggc cagcatgacc tcgccgtcgc gcgcatga
//
LOCUS       DASWGM010000071         6038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_421175,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000071 DASWGM010000000
VERSION     DASWGM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421175"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VGA15_02320"
     CDS             complement(<1..1220)
                     /locus_tag="VGA15_02320"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEX9586567.1"
                     /translation="MDQPRATWIKDPLGILADGAERGIVVRNGRIVELVPKGGQPTTP
                     AAFFEAGDHVVLPGLINTHHHFYQTLTRALPAALDRELFPWLQALYPVWARLTPEALN
                     LGVTVAMSELLLSGCTTTTDHHYVFPAGLEDAIDIEVAVANRLGVRVLLTRGSMNRSQ
                     RDGGLPPDSVVQDEDTILADSERVVARHHQRGEDAMVQIALAPCSPFSVTTSLMRATA
                     ALADGLDVRLHTHLAETEDENKFCEQMHGCRPLDYLEQCGWLNARTWLAHGIHFTAVE
                     MKRLGKAKTTISHCACSNQLLASGCCPVCDMEEAGVQIGLGVDGSASNDGSNLMQEVR
                     AAFLLQRARYGVGRVSHKDALRWATMGSAACVGRPELGEIAVGKAADLALFKLDELRF
                     SGHGDPLAALVLCGA"
     gene            1377..2204
                     /locus_tag="VGA15_02325"
     CDS             1377..2204
                     /locus_tag="VGA15_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9586568.1"
                     /translation="MDLNTIIEVAHPQTRGQLPVWTAGHAWLAGGTWLFSEPQVHLTR
                     LIDLTDFRWPALAIGDTGLTIAATCTVAQLDALACPPEWIAAPLIDQCCRAFLASFKI
                     WKTATVGGNLCMSLPAGPMIALTAALDGICTVWKADGSEQKIGVADFVTGNQSNVLTP
                     GDLLRQIDIPIPALKRRSAFRQISLTPVGRSAALLIGSVTGAGATALTVTASTTRPVQ
                     LSFPEIPAASELREAILHRIPDALYHNDVHGKSAWRKHMTLRLAQEIRAELLGGAPP"
     gene            2201..4936
                     /locus_tag="VGA15_02330"
     CDS             2201..4936
                     /locus_tag="VGA15_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9586569.1"
                     /translation="MSYQINGKEFSERPQAGQCLRTFLRELGHFGVKKGCDAGDCGAC
                     TVLIDGEPVHSCIVPAFRAEGHAVTTIEGLAPDSGTHPMQQAFLDAQAFQCGFCTSGM
                     ILTCASLNQAQRQDLGASLKGNLCRCTGYRAIEDALNGKTNTEGAAAGTAFGRSLPAP
                     AGPQLVRGAARYTFDTVIEGLLHIKMLRAPHPHAKIISIDKTAALAVAGVHAVLTFED
                     APDRLFSTARHEKAWMDPDDTRVLDNIVRFVGQKVAAVVAETEAAAEEGCRRLKVDYD
                     VLPDVIDPAQAIAAGAPRLHPDKTPDHRVANAQRNIVAETHGEYGDVARALAQAAVTY
                     EGTFTTQRVQHAALETHGGLAWIDPTGILNVRSSTQVPFLTLRALTEIFDLPPDKVRV
                     FCERVGGGFGGKQEMFVEDILALAAIRTGRPVKLELTREEQFISTSTRHPMRITVKAG
                     ADSHGRLTALQLDVLSNTGAYGNHAGPVLFHSCGESLGVYNCANKKVDAVVAYTNTVP
                     SGAFRGYGLPQTLLAVEAAIDELAKGLGISPYEIRRRNIVKPGDPMLSPPGPEHTDVF
                     YGSYGLDQCLDLVERAMRADGPNTELSADWLIGDGIALTMIDTVPPGGHLADARISLQ
                     DDGGFELTVGTAEFGNGTTTVHRQIAATALATTVDQIRLLQSDTAHGGHDTGAYGSAG
                     TFVAGRATQAAAENLADDLRVFASTATKTNPDACVLENDSAVCGRQRLSFSELAEAAR
                     AQGHALSATGTSAGTPRSVAFNVQGFRVAVNKFTGEIKILKSVHAADAGRVANPMQCR
                     GQIEGGVAQSLGATLYEEMVIDDRGRVVNPKFRDYHLPSFADIPRTEVFFADTTDSLG
                     PMGAKSMSESPYNPVAAALGNALADATGIRFTAVPFKPDRLWPMLHEKFGSKVGS"
     gene            complement(5214..>6038)
                     /gene="pucL"
                     /locus_tag="VGA15_02335"
     CDS             complement(5214..>6038)
                     /gene="pucL"
                     /locus_tag="VGA15_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEX9586570.1"
                     /translation="NKYGKGRVRVMRIHRDGDKHEVSQLNVKAMIEGDFARAYTHADN
                     STAFSTDTIKNVVNIVARQNTGLCTEEFCQVLAKKYLDTYPQVASVNVTAHETKWSRL
                     IFGGKPHPHSFVLDSNGKPSVEVTATREGSTLTSGIDGFTFMKSTQSGWENYVTDPYT
                     TIPETHDRMCATSMVASWKWSSKPANYPATNAKILATLLEVFGTTYSSSVQDSLYRMG
                     EAALVAVPEISEISMACPNMHFILMNLSAFGLDNNNDVFLPTDEPHGQIECTVGRG"
BASE COUNT         1119 a         1992 c         1857 g         1070 t
ORIGIN      
        1 gcgccgcaca ggaccagcgc cgccaatggg tcgccgtggc cggagaaacg caattcgtcg
       61 agcttgaaca gcgccagatc ggcggctttc ccgacggcga tctcgccgag ttcgggacgg
      121 ccgacacagg ccgccgatcc catggtagcc cagcgcaggg cgtccttgtg gctgacgcgg
      181 ccgacgccat aacgcgcccg ctgcaacagg aacgccgccc gcacctcctg catcagattg
      241 gatccatcgt tcgaggccga gccatcgacg ccgagcccga tttgaacccc cgcctcttcc
      301 atgtcacaga ccggacagca gccggacgcc agcagctggt tgctgcaggc gcaatggctg
      361 atggtcgtct ttgccttgcc gagacgtttc atctcgacag ccgtaaaatg gatgccatgg
      421 gccagccagg tccgggcatt gagccagccg cactgctcga gataatcgag cggccggcag
      481 ccatgcatct gctcgcagaa cttgttttcg tcctcggtct ccgccaggtg agtatgcagc
      541 cggacgtcga gcccgtcggc aagggctgcc gtcgcgcgca tcaacgacgt cgtgaccgaa
      601 aacggcgaac atggcgccag cgcgatttgc accatggcgt cctcaccgcg ctggtggtgc
      661 cttgccacca cgcgttcgct gtcggcgagg atggtatctt catcctgcac cacgctatcg
      721 ggcggcagtc caccgtcgcg ctgcgaccgg ttcatcgagc cacgcgtgag caacacgcgg
      781 acgccgagcc gattggcgac cgcgacctcg atatcgatgg cgtcctcgag cccggcagga
      841 aaaacataat gatggtcggt cgtggtggtg caacccgaca gcagcagttc cgacatcgcc
      901 acggtgacgc caaggttgag ggcctcagga gtgagccgcg cccacaccgg ataaagcgcc
      961 tgcagccagg gaaacaattc gcggtccagc gccgccggca gcgcgcgcgt cagcgtctgg
     1021 tagaagtgat gatgggtgtt gatcagcccc ggcagcacga catggtcgcc cgcctcaaag
     1081 aacgccgctg gtgtcgtcgg ctggccgcct ttgggcacca gctcgacgat cctgccgttc
     1141 cgcaccacga tgccgcgctc cgcgccatcg gcgagaatgc ccaagggatc cttgatccag
     1201 gtcgcccgcg gttgatccat ggcgtgaagc ttctccgaag gattaagatg cctgaaaagc
     1261 gttgccacac ttgcccttgc aattcaaaga ccaaccctat cctgaaagga cagttggcgt
     1321 cggttttgca agacttcagc agggagaagt atctcgttga aagctctggt atattcatgg
     1381 atctgaacac catcatcgaa gtcgcgcacc cgcaaacgcg gggacaactg cccgtttgga
     1441 cggcaggcca tgcttggctc gcgggcggca cctggctgtt ctcggaaccc caggtccatc
     1501 tgacgcggct gatcgatctc accgatttca gatggcccgc gctcgcgatc ggcgacaccg
     1561 ggttgacgat cgccgcgacc tgcacggtcg cccagcttga cgcgcttgcc tgcccgcccg
     1621 aatggatcgc cgcccccctg atcgaccaat gctgccgcgc ctttctcgcg tcattcaaga
     1681 tctggaaaac cgcgaccgtc ggcggcaatc tctgcatgtc gttgccggcc ggaccgatga
     1741 tcgcgctgac ggcggcgctc gatggcatct gtacggtctg gaaggctgac ggcagcgagc
     1801 agaaaatcgg cgtcgccgac ttcgtcaccg gcaaccagag caacgttctc accccgggcg
     1861 atctgctgcg gcagatcgac attccgatcc cggcgctgaa acggcgctcg gcgtttcgcc
     1921 agatatcgct gaccccggtc ggccggtcgg cggcgctgtt gatcggcagc gtcaccggcg
     1981 cgggcgccac ggcattgacg gtaacggcat ccacgacgcg gccggtccag ctctcgtttc
     2041 ccgaaattcc agccgccagt gaactgcgcg aagcgatcct gcatcgcatc cccgacgccc
     2101 tttaccacaa cgacgtccac ggcaagtcgg cctggcgcaa gcacatgacg ctgcgcctgg
     2161 cgcaagaaat tcgcgccgaa ttgctgggcg gcgcaccgcc atgagctatc agatcaacgg
     2221 caaggagttt tcggaacgtc cgcaggccgg gcaatgcctg cgaacttttt tacgcgaact
     2281 cgggcatttt ggcgtcaaga aaggctgcga tgccggcgat tgcggcgcct gcacggtgct
     2341 gatcgatggc gagccggtgc atagctgcat cgtcccggcg ttccgcgccg agggccatgc
     2401 cgtcaccacc atcgagggcc tcgcgccgga cagcggcacc cacccgatgc agcaggcgtt
     2461 tctcgatgcg caggcattcc agtgcggctt ctgcacctcc ggcatgattc tgacctgcgc
     2521 atccctgaac caggcgcagc gacaggatct cggcgcgtcg ctgaaaggca atctgtgccg
     2581 ctgcaccggc tatcgcgcga tcgaggacgc gcttaacggc aagaccaata ccgaaggcgc
     2641 tgccgccggc accgccttcg gccgcagcct gcccgcaccc gccggcccgc agctggtgcg
     2701 cggcgcggcg cgctacacct tcgacacggt gatcgaggga ttgctgcata tcaagatgct
     2761 gcgcgcgccg catccgcacg caaagatcat atcgatcgac aagaccgcgg cactggccgt
     2821 tgccggcgtg catgcggtgc tgaccttcga agatgcgccg gaccggctgt tctcgaccgc
     2881 gcggcacgag aaggcctgga tggatccgga cgacacccgc gtgctggaca atatcgtccg
     2941 cttcgtcggt caaaaggtcg ccgccgtggt cgcggaaacc gaggccgccg cggaggaagg
     3001 ctgccgccgc ctcaaggtcg actacgacgt tctgcctgat gtgatcgatc cggcgcaggc
     3061 gatcgcagcg ggcgcgcccc ggctccaccc cgacaagacg ccggaccatc gcgtcgcgaa
     3121 tgcgcaacgc aacatcgtgg cggaaaccca tggcgagtat ggcgatgtcg ccagggccct
     3181 ggcccaggcc gcggtcacct acgagggaac gttcaccacc cagcgcgtcc aacacgccgc
     3241 cctggaaacc catggcgggc tcgcctggat cgatccgacc ggcatcctga acgtgcgttc
     3301 cagcacgcaa gtaccattcc tgacgctgcg cgcgctgacg gaaatttttg acctcccgcc
     3361 ggacaaggtc cgggtgttct gcgaacgcgt cggcggcggt tttggcggca aacaggaaat
     3421 gttcgtcgaa gacattcttg ccctggccgc catcaggaca ggacggccgg taaaacttga
     3481 actcacccgc gaagaacaat tcatctcgac ctcgacgcgg catccgatgc gcatcaccgt
     3541 gaaggcaggc gccgacagcc atggaagact gacggcattg cagctcgacg tgctctcgaa
     3601 caccggggcc tatggcaatc atgccggacc ggtgctgttc cattcgtgcg gcgagtcgct
     3661 tggcgtctat aattgcgcca acaagaaggt cgatgccgtc gtcgcctata ccaacaccgt
     3721 gccgtcgggc gcgttccggg gttacggtct gccgcagacg ctgttggcgg tggaagccgc
     3781 gatcgacgaa ctggccaagg ggctcggcat cagcccttac gaaatccgcc gccgcaacat
     3841 cgtgaaaccg ggcgatccga tgctgtcgcc tcccggcccc gagcataccg acgtgttcta
     3901 tggcagctac gggctcgatc agtgcctcga tctcgtcgag cgcgcgatgc gggccgatgg
     3961 cccgaacacg gaactctccg ccgactggct gatcggcgac ggcatcgcac tgaccatgat
     4021 cgacaccgtg ccgccgggcg ggcatttggc cgacgccaga atttcgctgc aggacgatgg
     4081 cggcttcgaa ctgaccgtgg gaacggcgga gttcggcaac ggcaccacca cggtgcatcg
     4141 ccagatcgcc gcgaccgcgc tggcgaccac ggtcgatcaa atccgcctgc tacagtccga
     4201 caccgcgcat ggcggccacg acaccggcgc ctatggcagc gccggcacct tcgtcgccgg
     4261 acgcgcgacg caggccgcgg cggaaaatct ggccgacgat ctgagggttt ttgcttccac
     4321 cgcgacgaaa acaaatcccg atgcttgcgt gctggaaaac gattccgcgg tctgcggcag
     4381 gcaacggctg tcgttttccg agctcgccga agccgcccgc gcgcagggac acgcgctcag
     4441 cgcgaccggc acctcggcag gcacgccgcg gtcggtcgcg ttcaacgtcc agggctttcg
     4501 cgtcgccgtg aacaagttca ccggggaaat caagatcctc aaaagcgtcc acgccgcgga
     4561 tgccggccgg gtcgccaatc cgatgcagtg ccgcggacag atcgagggcg gggtcgcgca
     4621 gtcgctcggc gccacgctct atgaggagat ggtgatcgat gaccgtggcc gcgtggtcaa
     4681 tccgaaattc cgcgactatc atttgccgtc gttcgccgat atcccgcgca ccgaagtgtt
     4741 tttcgcggat accaccgata gcctggggcc gatgggcgcc aaatcgatga gcgaaagtcc
     4801 ctacaatccg gtcgccgcag cgctcggcaa tgcactggcc gatgccaccg gcatccgctt
     4861 cacggcggtg ccgttcaagc ccgaccggct atggccgatg ctgcatgaga agtttggtag
     4921 taaggtcgga tcgtagaaac gcaccgctcc ttcacctctc cccgctcttt gcggggagag
     4981 gtcggatcgc ccttgcgatc cgggtgaggg gcaaggcact gctttaccga ggcgtagacc
     5041 tctcgggtcg cgaagcaagc gctatggtac tcctgcggtc tgagcggcgc gatttgcgcg
     5101 acctgagagt ccttccattg tgtcgagcgc aatgcccggc ccctcacccg aagccttcgc
     5161 tacgcttcgg cttcgacctc tccccgcaaa gagcggggcg aggtatagaa acatcacccc
     5221 cgacccaccg tgcattcgat ctggccgtgc ggctcgtcgg tcggcagaaa gacgtcgttg
     5281 ttgttatcca gcccgaacgc cgacaggttc atcaggatga aatgcatgtt cgggcacgcc
     5341 atgctgatct ctgaaatttc cggcactgcc accagcgccg cctcgcccat cctgtacagg
     5401 ctgtcctgca cgcttgaact gtaggtggtg ccaaaaacct ccagcaatgt cgctagaatt
     5461 ttcgcgttgg tggccgggta gtttgccggc ttcgacgacc atttccacga tgccaccatg
     5521 ctggtcgcgc acattcggtc gtgggtctcg gggatcgtcg tatagggatc cgtgacgtaa
     5581 ttctcccagc ccgactgggt cgacttcatg aacgtaaagc cgtcgatacc cgaggtcagc
     5641 gtcgatccct cccgcgttgc ggtcacctcg actgacggct tgccgttgct gtcgagcacg
     5701 aaactgtggg ggtgcggctt gccgccgaaa atcaagcggc tccattttgt ctcatgcgcc
     5761 gtcacgttga ccgaggccac ctgcggatag gtatcgagat atttcttcgc cagcacctgg
     5821 cagaattcct cggtgcagag gccggtgttc tggcgcgcga cgatattgac cacattcttg
     5881 atggtgtcgg tagagaacgc cgtggaattg tcggcatgcg tataggcgcg cgcaaagtcg
     5941 ccctcgatca tcgccttgac gttgagctgg ctgacctcgt gcttgtcgcc atcacgatga
     6001 atccgcatga cgcggacccg gcctttgcca tatttgtt
//
LOCUS       DASWGM010000072         6782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1808454,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000072 DASWGM010000000
VERSION     DASWGM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808454"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..114
                     /gene="ffs"
                     /locus_tag="VGA15_02340"
     ncRNA           18..114
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGA15_02340"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            236..2086
                     /locus_tag="VGA15_02345"
     CDS             236..2086
                     /locus_tag="VGA15_02345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX9586571.1"
                     /translation="MTEAGIPVTPPTNEPDSSHRGGFGLGGPPEAGKHYRVLARKYRP
                     SSFEDLIGQDAVVRTVSNAFETGRIPQAWILTGVRGVGKTTTARILARALNYELPDGS
                     VKGPTIQMPKLGVHCQAIMESRHMDVLEMDAASHTGVDDVRQINDSVRYAPASARYKV
                     YIIDEVHMLSNQAFNAFLKTLEEPPEHVKFVFATTEIRKVPVTVLSRCQRFDLRRVEA
                     DVLMGHLANIAARENVEAEPEALGIIARAAEGSVRDSLSLFDQAIAHAAGVVRADAVR
                     QMLGLADRTRVIDLFDSLARGDIASAFKEFREQYDTGADPIVVLSDLAEFVNFVTRVK
                     IVPATADNVAFGETERLRARDFAAKLSMRVLSRMWQMLLKGITEVQTATRPAAAAEMV
                     LVRIAYVADMPTPDEAIRMLEQNGGASPVIAGAAASRSAAAPTVSSIQPSAARASAPR
                     ASAEASARPQMIAPVADAQSAPAARKISSFPELVALAGEKRDLLTKAALEADVRLVRI
                     EDGRLELAMERSAARTLINDLSRKLEQWTERRWTVIVSNEPGQPTLRSQNELQKSQRE
                     RAAESDPRVQEVLTRFPGAKVVEVRKLAPEPPESDASGEDPAENSDSDDQ"
     gene            2108..2428
                     /locus_tag="VGA15_02350"
     CDS             2108..2428
                     /locus_tag="VGA15_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX9586572.1"
                     /translation="MADFLGMMKQAAQLQSKMQAMQEELGHVEVEGISGGGLVTVRMT
                     AKMQVKAVKIDPSLMKADEREILEDLLVTAHNDAQRKAEAAMQEKMQALTGGLGLPPG
                     LGLT"
     gene            2430..3035
                     /gene="recR"
                     /locus_tag="VGA15_02355"
     CDS             2430..3035
                     /gene="recR"
                     /locus_tag="VGA15_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX9586573.1"
                     /translation="MAAAVAGPEIERLIQLLARLPGLGPRSARRAALHLIKKREALMT
                     PLSAALQVAIDKIQVCKTCGNIDTQNPCTVCTDPRRDPAIIVVVADVADLWALERAHA
                     TNGFYHVLGATLSPLDGVGPQDLTIDALVARAHDPRVTEIILALNATVDGQTTAHYIT
                     DLLQEAGVKVTRLAHGVPVGGELDYLDEGTLSAAMRQRTLF"
     gene            3055..3486
                     /locus_tag="VGA15_02360"
     CDS             3055..3486
                     /locus_tag="VGA15_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586574.1"
                     /translation="MTRVRFGRQLALAAFVAATLAPPAVLAQQAEPAPKPGKMINAGD
                     VLSGELNTLRMRGGKKGKPAATYQLTSEPRRLPPPNGLCNLETGPETFQLITSSEAQA
                     AQLKGFIGKEISVKVGEIACAQDAGQMSEAVVTKWSVVTKH"
     gene            complement(3682..5046)
                     /locus_tag="VGA15_02365"
     CDS             complement(3682..5046)
                     /locus_tag="VGA15_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9586575.1"
                     /translation="MTAPEAATPEKAGAPRASWREGLAVYLQRRVLIVLLLGFSSGLP
                     LALSGSTLLVWMRESGVDLGTIGLFALVGTPYTLKFLWAPLVDALHVPFFTQQFGRRR
                     GWLVFSQLLLIGAILLLALTDPARSPLFVALGALLVAAMSSTQDIVVDAFRVESLPES
                     EQAAGMASYVAAYRIGMLVSTAGALFMVSAFEGMGLARASAWMWGYVAMAALVLIGTV
                     TALAATEPGQSARAEAATSTESAFTRVMHAAIGAFSEFLVRKDALAALAFVVLFKFTD
                     AFSGTMTAPFVIDLGFSRNDYAAIVKGVGLAATLIGGFAGGFVARRYSLAASLWIGGV
                     LQAVANLSFSWLAIVGVNQWALAFAITAENFTSAIGTVIFVAYLSALCKNPLHTATQY
                     ALLTALAAVGRTYLSSGAGYVAKATGWPLFFAICVVVAVPSLILLAWLQRRGHFATLE
                     PVRV"
     gene            5133..5432
                     /locus_tag="VGA15_02370"
     CDS             5133..5432
                     /locus_tag="VGA15_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9586576.1"
                     /translation="MGARGYYVYILTNRIGGTLYIGVTNDLVRRVYEHKSNAAESFTK
                     RHDVHRLVYFECFDDIEQAIHREKRLKKWPRAWKISLIEKDNPDWIDLYPEIAGG"
     gene            complement(5549..6781)
                     /gene="ltrA"
                     /locus_tag="VGA15_02375"
     CDS             complement(5549..6781)
                     /gene="ltrA"
                     /locus_tag="VGA15_02375"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009495311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX9586577.1"
                     /translation="MVERDNLRKALAHVKRNKGAAGIDGMSLDALAPHLKEHWPAIRA
                     QLLEGTYKPQPVRRVEIPKASGGTRSLGIPTVLDRFIQQAVLQVLQAEWDPTFSEHSF
                     GFRPKRSAHQAVARAQELIASGHSIVVDLDLEKFFDRVNHDILMGLVAKRVADKRVLK
                     LIRGFLTAGVLMDGLVGPTEEGTPQGGPLSPLLSNLMLDVLDKELERRGHNFVRYADD
                     CNVYVRSQRAGERVMVSVERFLTKRLKLKVNKAKSAVARPVKRKFLGFSFTNGKQPRR
                     RIAPQSLARFRSRVRELTRRTRGSSLAQIIKELSVYLIGWRGYFGFCETPSVLRELDQ
                     WTRRRLRAIVWKQWKSGPTRFAKLRRCGVGPVLAAKAASSPRGPWRLSNSPALAIALS
                     NAVLKSLGLATVAVPKPA"
BASE COUNT         1356 a         2116 c         2170 g         1140 t
ORIGIN      
        1 gccccaaata aggaccggga gattggcggt ggacgagcca ctcgccaacc gggtcaggtc
       61 cggaaggaag cagccctaac gaggtccgga tcgggtcgct cgtcagtctc ctacctgttt
      121 tttcgagcga atcgcgcaag aacgctgacc aggagcggtg acttggcccg cgtccggctc
      181 gcgtcgttgt tccgcaagcc gacttcgaga gactatcaat tttgcggatc gatcgatgac
      241 cgaggctggc attcctgtaa cacccccgac gaacgagccg gacagctccc atcgaggcgg
      301 cttcggcctc ggcggcccgc cggaagccgg caagcactat cgtgtgctgg cgcgaaaata
      361 ccggccctcc agtttcgaag atttgatcgg ccaggacgcc gtggtccgta ccgtttcgaa
      421 tgcgttcgaa accgggcgga tcccgcaagc ctggattttg accggggtgc gcggcgtcgg
      481 gaaaaccacc acggcgcgga ttcttgcccg cgcgctgaat tacgaactgc cggacggttc
      541 ggtgaaggga ccgaccatcc agatgccgaa actcggcgtg cattgccagg cgatcatgga
      601 aagccggcac atggacgtgc tggaaatgga tgccgcatcc cataccggcg tcgatgatgt
      661 gcgccagatc aacgacagcg tgcgctatgc gccggccagc gcgcgctaca aggtctacat
      721 catcgacgaa gtccacatgc tctcgaacca ggcgttcaac gcgttcctga agacgctgga
      781 agagccgcct gagcacgtca aattcgtgtt cgcgaccacg gaaatccgga aagtgccggt
      841 cacggtgctg tcgcgctgcc agcgttttga cttgcgccgg gtcgaggccg acgtgctgat
      901 gggccatctc gccaatatcg cggccaggga aaacgtcgag gcggagccgg aggcgctcgg
      961 catcatcgcg cgcgcggcgg agggttcggt gcgtgattcg ctgtcgctgt tcgatcaggc
     1021 gattgcgcac gccgcgggcg tggtgcgtgc cgacgccgtg cggcagatgc tcgggcttgc
     1081 cgaccgtacc cgggtgatcg atctgtttga ttcactggcg cgcggcgaca tcgccagcgc
     1141 gttcaaggaa ttccgcgagc aatatgacac cggcgccgac ccgatcgtgg tgctgtccga
     1201 tcttgccgaa ttcgtcaatt tcgtcacccg cgtcaagatc gtgccggcga cggcggacaa
     1261 tgtcgcattt ggcgaaaccg aacggttgcg cgcccgcgac tttgccgcaa aactctcgat
     1321 gcgggtgctg tcgcggatgt ggcagatgct gctgaagggc atcaccgagg tgcagaccgc
     1381 gacgcgcccg gcggcggcgg ccgaaatggt gctggtgcgc atcgcctatg tcgcggacat
     1441 gccgacgccg gatgaagcga tcaggatgct cgaacaaaat ggcggtgcat cgccggtcat
     1501 cgccggcgcc gcggcttcac ggagtgctgc ggccccgacg gtatcctcca tacagccttc
     1561 ggcggcgcgc gcttcggcgc cgcgcgccag tgccgaggcg tcggcgcgcc cgcagatgat
     1621 cgcgccggtc gccgacgcgc agtccgcacc cgcggcgcgc aagatttcga gctttccgga
     1681 actggtggcg cttgccggcg agaagcgcga cctgctgaca aaggccgcgc tggaggccga
     1741 tgtccgcctg gtccgcatcg aagacggacg gctcgagctg gcgatggaac gcagcgcggc
     1801 gcggaccctg atcaacgatc tttcccgtaa actggaacaa tggaccgaac ggcgctggac
     1861 cgtgatcgtg tccaacgaac ccggacaacc gacgctgcgg tcgcaaaacg aattgcagaa
     1921 gagccagcgc gagcgcgccg ccgaatccga tccccgggtg caggaggtgc tgacgcgatt
     1981 tcccggcgcc aaggttgtcg aggtacgcaa gcttgccccc gagccgccgg aatccgatgc
     2041 tagcggtgaa gatccggccg agaattctga cagcgacgat caatagcaat ctcaagagga
     2101 acggttgatg gctgattttc tcggcatgat gaagcaggcg gcgcaactgc aatcgaagat
     2161 gcaggcgatg caggaagaac tcggccatgt cgaggtcgag ggcatctcgg gcggcggtct
     2221 cgtcacggtg cgcatgacgg ccaagatgca ggtgaaggcg gtgaagatcg acccctcgct
     2281 gatgaaggcg gacgagcgtg aaatcctcga agacctgctg gtcaccgccc ataacgacgc
     2341 gcagcgcaag gcggaagccg cgatgcagga gaagatgcag gccctgaccg gcggcttggg
     2401 tctgccgccg gggctgggcc tcacctgaca tggctgctgc cgtcgccggt cccgaaatcg
     2461 aacgcctgat ccagctgctg gcgcggctgc cggggcttgg cccgcgctcg gcgcggcgcg
     2521 ccgcgttgca tctgatcaag aagcgcgaag cgctgatgac gccgctctcc gccgcgctgc
     2581 aggtggcgat cgacaagatc caggtttgca agacctgcgg caatatcgac acccagaatc
     2641 catgcacggt gtgcaccgac ccgcggcggg accctgcgat catcgtcgtg gtcgccgacg
     2701 tcgccgatct gtgggcgctg gaacgggcac acgcgaccaa tggcttctat cacgtgctgg
     2761 gcgcgacgct gtcgccgctc gatggcgtgg gcccgcagga tctgaccatc gacgcgctgg
     2821 tggctcgcgc ccacgacccg cgggtgacgg aaatcattct ggcgttgaat gcgaccgtcg
     2881 acggccagac caccgcgcat tacatcaccg acctgctgca ggaagcgggc gtcaaggtga
     2941 cgcggctcgc ccacggcgtg ccggtcggcg gtgaactcga ctatctcgat gaaggcacgc
     3001 tgtccgcggc gatgcggcag cgtacgttgt tttgaaaact gaatcagagg gcacatgact
     3061 agagtaagat tcggcaggca attggctttg gcggcattcg ttgcggcaac gcttgccccg
     3121 cctgccgtcc tggcgcagca ggccgagcct gcgccgaagc ccggcaagat gatcaatgcc
     3181 ggcgacgtgc tgtcgggaga gctcaacacg ctgcgcatgc gcggcggcaa aaagggcaaa
     3241 ccggccgcca cctatcagct caccagtgag ccacgccggc tgccgccgcc gaacggactc
     3301 tgcaatctcg aaaccggacc ggagacgttc cagctcatca ccagcagcga ggcccaggcg
     3361 gcgcagctga agggctttat cggcaaggaa atttcggtga aggtcggcga gatcgcctgc
     3421 gcgcaggacg ccgggcagat gagcgaggcc gtggtcacga aatggagcgt ggtgacgaag
     3481 cattgaggaa tgctgctgtt actgcaagca atgctgtcgt cacaaaaaat gctgtcatcg
     3541 cccggcttga ccgggcgacc cagtattgca gagcgtcgac gatggatcgc agcgctccgg
     3601 aatactggac cacccgcttt cgcgggtggt gacggccaaa gttgtataac ggcagtgctc
     3661 cgccgtgcac ttaccgaatc gctaaaccct taccggctcc agcgtcgcaa aatgcccgcg
     3721 ccgctgcagc cacgccagca ggatcaggct cggcaccgcg accacgacgc agatcgcaaa
     3781 aaacagcggc caccccgtcg ccttggccac gtaacccgcg ccggatgaca aataggtgcg
     3841 cccgaccgcg gcaagcgcgg ttaagagcgc atattgcgtc gcggtgtgca gcggattttt
     3901 gcatagcgcc gagagatagg cgacgaagat cacggtgccg atggcgctgg tgaaattttc
     3961 cgccgtaatg gcgaaggcca gcgcccattg attgacgccg acaatcgcca gccacgagaa
     4021 cgacagattg gcgaccgcct gcagcacccc gccgatccac aggctcgcgg ccagcgaata
     4081 gcgccgcgcg acgaatccgc cggcaaaacc gccgatcagg gtggcggcga ggccgacacc
     4141 cttgacgatg gcggcgtagt cgttacggga aaagccgagg tcgattacga atggcgcggt
     4201 catggtaccg gaaaacgcat cggtgaattt gaacagcacc acgaaggcaa gtgctgcaag
     4261 cgcatccttg cgaacgagaa attcagagaa ggcgccgatc gcggcatgca tcacgcgggt
     4321 gaacgcggac tcagtgctgg tcgcggcctc cgcgcgcgcc gattgcccgg gctccgtcgc
     4381 ggccagcgcc gtgacggtgc cgatcagcac caacgctgcc atcgccacat agccccacat
     4441 ccatgccgag gcgcgcgcga gacccatgcc ttcgaacgcg ctaaccatga acagcgcgcc
     4501 cgcggtcgag accagcatgc cgatccgata ggctgcgaca taggacgcca tgccggcggc
     4561 ctgctcgctc tcgggcaggc tctcgacgcg gaacgcatcg accacgatgt cctgcgtaga
     4621 tgacatcgcc gcgaccagca gcgcgccgag cgccacgaac agcggcgaac gcgccgggtc
     4681 ggtcagcgcc agcagcagga ttgcgccgat cagcagcagt tgcgagaaca ccagccagcc
     4741 acggcggcgg ccgaattgct gcgtgaagaa cggcacatgc agcgcgtcga ccagcggcgc
     4801 ccagagaaat ttcagcgtgt agggcgtgcc gaccagcgcg aacaggccga tggtgccgag
     4861 atcgacgccg gattcgcgca tccacaccag gagcgtcgat cctgacagcg ccagcggcag
     4921 gccggacgaa aagccgagca gcaagacgat cagcacccgc cgctgcagat acaccgcgag
     4981 cccctcgcgc caggaggcgc ggggggcgcc ggctttttcg ggtgttgcgg cttccggtgc
     5041 ggtcatgaaa aggtgttagc agattctgat gcaacaaatg atactgtcat cgcccggctt
     5101 gaccgggcga cccagtattc cagagacggt tgatgggagc acgcggttac tacgtttaca
     5161 tacttactaa tcgcatcgga ggtacgcttt acatcggcgt gaccaacgac ctcgttcgtc
     5221 gcgtgtatga gcacaaatcg aatgcggccg aaagctttac caaacgtcac gatgtgcatc
     5281 ggctggttta tttcgaatgc ttcgacgaca ttgaacaggc tatccatcgt gagaagcggc
     5341 tgaagaaatg gccgcgtgcc tggaagattt cattgataga gaaagacaat ccagactgga
     5401 ttgatcttta tcctgaaatt gccgggggat gaaggtacgt cagcggctta gacggagagg
     5461 tctctggaaa tcgggatagg gggagtctcg cgactccgcc cctcccacac caccgtacgt
     5521 acgggtccgt atacggcggt tcgacagatt aagctggctt gggtaccgca acagttgcaa
     5581 ggccgagcga tttgagtacg gcgtttgata gagcaatggc aagtgcgggg ctgttgctga
     5641 gccgccaagg gccacgtggg ctgctggcgg ctttcgccgc cagaaccggg ccgacgccgc
     5701 agcgtcgcag tttcgcaaag cgagtgggcc cgcttttcca ttgcttccaa acgatggcgc
     5761 gcagccgtcg tctggtccat tggtcaagtt cacgcaacac cgatggcgtc tcgcagaatc
     5821 cgaagtagcc gcgccacccg atgaggtaga cggacagctc tttgatgatc tgcgcgaggc
     5881 tgctgcctcg ggtccgacgc gtcagttccc gtacccggct ccggaatcgg gccagcgact
     5941 gcggcgcgat gcgccgccga ggctgtttcc cattcgtgaa gctaaagccc aggaatttgc
     6001 gcttaaccgg tcgggcgacc gcgctcttcg ccttgttgac cttcagctta aggcgcttgg
     6061 tcaagaacct ctcgacgctg accatcactc gctcgccggc ccgctgactg cgaacgtaga
     6121 cgttgcagtc gtcggcatag cgcacgaagt tgtggccgcg cctctccagt tccttatcca
     6181 gcacatcaag catcagattc gacagcagcg gcgagagcgg cccaccttgt ggtgtacctt
     6241 cctccgtcgg accgaccaac ccatccatca gcacgcctgc ggtcagaaaa ccgcggatca
     6301 gcttcagaac gcgcttgtcg gccacccgtt ttgccaccaa ccccatcagg atgtcgtggt
     6361 tgacccggtc gaagaacttt tccaggtcga gatccactac gatgctatga ccggacgcga
     6421 tcaactcttg cgcccgagcc accgcctgat gcgccgagcg cttcggccgg aagccaaagc
     6481 tgtgctcgga gaacgtcggg tcccattctg cctgcagcac ctgcaaaact gcctgctgga
     6541 tgaagcggtc gagcaccgtc ggaatgccga gcgaccgtgt gccgcccgat gcctttggta
     6601 tctccacacg cctcaccggc tgcggcttgt aagtgccctc aagcagctgg gcccggatcg
     6661 cgggccagtg ctctttcagg tgaggagcca gggcatcgag gctcatgcca tcgataccgg
     6721 cagctccttt gttgcgtttc acatgcgcta acgctttcct cagattgtct cgctcgacaa
     6781 tc
//
LOCUS       DASWGM010000073        10430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1944578,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000073 DASWGM010000000
VERSION     DASWGM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944578"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VGA15_02380"
     CDS             <1..377
                     /locus_tag="VGA15_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9586578.1"
                     /translation="ETPQWYARLARKDYTIGLNVTGVSVDDPDGNIVENYSCKSERNY
                     TQYCNAEVDQLLAAQSRELDKEKRRKIVWDIERLLVDDAARPNIISNVAVNCWQPYVR
                     NYIPHDNSQYNSLRFEDVWLDR"
     gene            complement(508..687)
                     /gene="gltD"
                     /locus_tag="VGA15_02385"
                     /pseudo
     CDS             complement(508..687)
                     /gene="gltD"
                     /locus_tag="VGA15_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
     gene            complement(1289..2746)
                     /locus_tag="VGA15_02390"
     CDS             complement(1289..2746)
                     /locus_tag="VGA15_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX9586579.1"
                     /translation="MGKITGFLEIDRRDRKYAPVAERVKGYHEFVIPLSEKDTRDQAA
                     RCMNCGIPYCHGTGSVAPGTPGCPVNNQIPDFNDLVYSGNWEEASRNLHSTNNFPEFT
                     GRICPAPCEASCTLNIDDNPVTIKTIECAIVDRAWDNGWVTPEIPTTKTGKKVAVIGS
                     GPAGLACAQQLARVGHEVHLFERWAKAGGLLRYGIPDFKMEKHVIDRRVAQMEAEGVT
                     FHYGAQVGGTSPGALDPREMLNEYDALALTGGAEAARDLPIPGRELAGIHFAMDFLPQ
                     QNRRVSNEPLGSAPDILAGGKHVVVIGGGDTGSDCIGTSFRQGALSVTQLEIMLAPPE
                     HENKGLTWPNWPLKMRTSSSQAEGAKREYAVLTQNFSGADGKVAKLHCVQVDDKFKPI
                     PGTAFELETQLVLLAMGFVHPVHEGLLKTLGVQLDPRGNVRANTADYQTSRPNVFSAG
                     DMRRGQSLVVWAIREGRLCARAIDEFLMGTTNLPR"
     gene            complement(3001..7737)
                     /gene="gltB"
                     /locus_tag="VGA15_02395"
     CDS             complement(3001..7737)
                     /gene="gltB"
                     /locus_tag="VGA15_02395"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX9586580.1"
                     /translation="MSGSEFERENTVTDALSAAPASKPDQPAREHNWRPPAEGLYDPS
                     LEKDSCGVGFIANIKGKKSHQIICDAINILCNLEHRGAVGADPRAGDGAGILVQIPHT
                     FFKRKAAEIGFQLPKPGDYAVGALFMPKETAWRKVIKSIIADQIKAEQLMLLGWRDVP
                     VDNSSLGETVKPTEPANMQVFIGRGDHIKTEDEFERRLYILRKSISQAIYLRRDRGLA
                     GYYPASMSCRTVIYKGMFLADQLGKYYPDLSEPDFESALALVHQRFSTNTFPAWSLAH
                     PYRMIAHNGEINTLRGNVNWMAARQASVHSELYGKDISRLWPISYEGQSDTACFDNGL
                     EFLVQGGYSLPHAVMMMIPEAWAGNPLMDETRRAFYEYHAAIMEPWDGPAAIAFTDGR
                     QIGATLDRNGLRPARYLVTSDDRIVMASEMGVLKIPEEHIVTKWRLQPGKMLLVDLEQ
                     GRLIPDDEIKAQLANSHPYRDWLDRTQIVLEDLPDAPTKGMRSNLPLLDRQQAFGYSQ
                     EDVTILMTPMASTGEEANGSMGNDTPISALSDKPKPLFTYFKQNFAQVTNPPIDPIRE
                     ELVMSLVSIIGPRPNLFDLQGMAGLTKRLEVRQPILTDADLEKVRSISEIADTHFKSR
                     TLDTTFHAGFGAAGLEQVLDELCARAEAAVREGINIVILSDRMAGSDRIPIPSLLACA
                     AVHHHLIRTGLRTSVGLVVESGEPREVHHFACLAGYGAEAINPYLAFETIIAMKDRLP
                     AALDDYEIVKRYIKSIGKGLLKVMSKMGISTYQSYCGAQIFDAVGLKADFVSKYFVGT
                     HTQIEGVGLAEIAEETARRHTDAFGDAPMYKTALDVGGEYAYRTRGEEHAWTAESVST
                     LQHAVRGNSLERYKAFAKILNEQSERLLTLRGLFKIKPAEHEKRKPVPLEQVEPAKDI
                     VRRFATGAMSFGSISREAHTTLAIAMNRIGGKSNTGEGGEESGRFKPMPNGDSMRSAI
                     KQVASGRFGVTTEYLVNADMMQIKMAQGAKPGEGGQLPGHKVDAVIARVRHSTPGVGL
                     ISPPPHHDIYSIEDLAQLIYDLKNVNPDGLVSVKLVSEIGVGTVAAGVAKARADHVTI
                     AGFEGGTGASPLTSIKHAGSPWEIGLAETHQTLVRERLRSRILVQVDGGFRTGRDVVI
                     GALLGADEFGFATAPLIAAGCIMMRKCHLNTCPVGVATQDPVLRKRFTGQPEHVINYF
                     FFVAEEVREIMAALGYRTFNEMVGQTQMLDQSTLVAHWKAKGLDFSKLFVRQQELPGQ
                     KIYHSQGQNHHLEAVLDRRLIEQSQAAIDRGAPVKIEMAINNTDRSAGAMLSGTVAKI
                     YGHAGLPHDTIHVGFKGTAGQAFGAWLARGITFDLEGEGNDYVGKGLSGGRIIVKPPR
                     NSGIVPEESIIVGNTVMYGAIEGECYFRGIAGERFAVRNSGAVAVVEGAGDHCCEYMT
                     GGIVVVLGKTGRNFAAGMSGGVAYVLDEAGDFEKLCNMAMVQLEPVLSEEMINANTYH
                     QSGDLEAHGRVDVFQDLLESDVERLHILITRHAKLTGSKRAADILANWKTSLPKFRKV
                     MPVEYRRALKELKANADAEPKIAIGA"
     gene            8159..8974
                     /locus_tag="VGA15_02400"
     CDS             8159..8974
                     /locus_tag="VGA15_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586581.1"
                     /translation="MKFFPGLAVSAALVCAVPAADAQTLAPYDAGKGRFVSDVDGPYV
                     DVPEAPPPAPRHYGYGRDRGYAPDRGYAPAYRDAPARGYGPDYGYAPAMLPPQEIYAI
                     LRENGFSPLGIPRQRGYVYVIAVLDRGGEDGRLTIDGRNGRIIRFVPASQWGEAYDRM
                     SFGRGPDRGSDRSRDHAPFSGAAVGALPTPMPINATPRPPTSLPSVAIRGAVPAQAQR
                     PAVVATKPPAQPVQQTAAKPAEAPKQAGTVGEAKPAAPQIKPSQEMPAVQGLE"
     gene            complement(9053..9829)
                     /locus_tag="VGA15_02405"
     CDS             complement(9053..9829)
                     /locus_tag="VGA15_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9586582.1"
                     /translation="MKIARVVIALTVAALAATLSGFDAHAQTSAPTEARNVVLVHGAW
                     ADGSSWAAVIPYLQAAGLKVTAVQNPLTSLEDSVAATRRALALQDGPTVLVAHSWGGT
                     VISETGIDPKVTSLVYVAARAPDAAEDFVALSAKFPTGPVRAGVQEHDGFTKLSEESF
                     LKYFANGVEPKAAEVLYAVQQPTAATLFGGRTTAAAWRSKPSWYAVSKLDQTINPDLE
                     RFLAKRMNATTVELEAGHLSLVSHPKQIADLILAAAGQKK"
     gene            complement(10302..>10430)
                     /locus_tag="VGA15_02410"
     CDS             complement(10302..>10430)
                     /locus_tag="VGA15_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEX9586583.1"
                     /translation="GLLHVDLVREIPEAKKPRSIPITTTSAVAPQVADNSAEKAAA"
BASE COUNT         1836 a         3242 c         3351 g         2001 t
ORIGIN      
        1 tggaaacgcc gcaatggtat gcgcggctcg cgcggaagga ttacacgatc ggactgaacg
       61 tcaccggcgt cagcgtcgac gatcccgacg gcaatatcgt cgagaattat tcctgcaaat
      121 cggagcgcaa ttatacccag tactgcaatg ccgaggtgga tcaactgctc gccgcgcagt
      181 cccgcgaact ggacaaggag aaacgccgga aaatcgtctg ggatatcgag cgtcttctgg
      241 tcgacgacgc ggcgcgcccg aacatcatct ccaatgtggc cgtgaactgc tggcaaccct
      301 atgtcaggaa ttacattccg cacgacaaca gccaatacaa cagcctgcgg ttcgaggacg
      361 tctggctgga caggtagggg cgtctacggg agcgcgcggg tgggttcgtt cggtgtcata
      421 cgcgggcttg acccgcgtac ccatcaatct tcgtaagagt cttgcaaaga agatgggttg
      481 cctggtcaag cccggcaacg gcggcagtta cctcggcaga ttcgtcgtcc ccatcaaaaa
      541 ctcgtcgatg gcgcgggcgc agaggcggcc ctcgcggatc gcccacacgg caagcgattg
      601 gccgcgccgc atatcgctgg cggagaacac gttgggccgc gaggtctgat agtcggcggt
      661 gttggcgcgg acattgccgc gcgggtctga cgtttccagt tacttcggcg gatgtagttc
      721 gccggtgaac caaccaaccg catcggattt catcggatct gacgctatcg cgatctgttc
      781 gacatgatcg catttggggc agtcgaacgt gcgaagttcg aatccgatag gcccgggcaa
      841 gatgcgcgta agcgtcatgc gcgttcggca ttgcgggcag tggaggtgct gaattgcgag
      901 gagtgggatt gcagctgggt aatcggtcat tgcattcccc ttggattggg cgggagcgca
      961 aaactctcag tcaccggtag aagccgaaga ggccgatgat gggatcatta gattcgctat
     1021 ccgtccggga gtcgacttga gaaacattaa ttcggcgaac cccagagagc gttctccaac
     1081 cggcaacgtg gtgctgccaa taccggtaaa atcagcgcct actgcgctgt gcaacaatgt
     1141 tgcgataccc ggccatcggg tctcggcttt ggcgagcccg aatgacaggc tccagcgggg
     1201 tatccagtac gccgcggcct cgcgattctt cactggcgct ctggaatact gggcccccgc
     1261 tttcgcgggg gcgacgaggc ggcgtcgctc acctcggcag attggtcgtg cccatcaaaa
     1321 actcgtcgat ggcgcgggcg cagaggcggc cttcgcggat cgcccagacg acgagcgatt
     1381 ggccgcggcg catgtcgccg gcggagaaca cgttgggccg cgaggtctgg tagtcggcgg
     1441 tgttggcgcg cacattgccg cgcgggtcga gctggacgcc gagcgtcttc aacaggccct
     1501 catgcaccgg atgcacaaag cccatcgcca gcagcaccag ttgcgtctcg agctcgaatg
     1561 cagtgccggg gattggcttg aacttgtcgt cgacctgcac gcaatgcagt tttgccacct
     1621 tgccgtcggc tcctgaaaaa ttctgcgtca gcaccgcgta ttcgcgcttg gcgccttcgg
     1681 cctggctcga cgaggtccgc atcttcaagg gccagttcgg ccaggtcagg cccttgttct
     1741 cgtgctcggg cggcgccagc atgatttcga gctgcgtcac cgacagcgcg ccctgccgga
     1801 acgaggtgcc gatgcagtcc gagccggtat cgccgccgcc gatcaccacc acatgcttgc
     1861 cgccggccag aatgtcgggc gcgctgccga gcggctcgtt actgacgcgg cgattttgtt
     1921 gcggcaaaaa atccatcgcg aagtgaatgc ctgcgagctc gcgtccgggg atcggcagat
     1981 cgcgcgcggc ctccgcgccg ccggtcagcg ccagcgcgtc atattcgttc agcatctcgc
     2041 gcggatcgag cgcgccggga gacgtgccgc cgacttgggc gccgtaatgg aacgtcacgc
     2101 cttcggcttc catctgcgcc acgcggcggt cgatgacgtg cttttccatc ttgaaatcgg
     2161 gaatgccgta gcggagcagc ccgccggcct tggcccagcg ctcgaacaga tgcacctcgt
     2221 ggccgacgcg cgccagctgc tgcgcgcagg cgagtcccgc cgggcctgaa ccgatcaccg
     2281 cgaccttctt gccggtcttg gtggtcggga tttccggcgt gacccagcca ttgtcccagg
     2341 cgcgatcgac gatggcgcat tcgatggtct tgatcgtgac cggattgtcg tcgatgttga
     2401 gcgtgcagga ggcttcgcac ggcgccggac agatgcggcc ggtgaattcc ggaaagttgt
     2461 tggtcgagtg cagattgcgc gaggcctctt cccagttgcc gctatagacc agatcgttga
     2521 aatccggaat ctggttgttg accgggcagc ccggcgtgcc cggggccacc gagcccgtgc
     2581 catggcaata gggaatgccg caattcatgc agcgcgcggc ctggtcgcgg gtgtccttct
     2641 cgctcagggg gatgacgaac tcatgatagc ccttcacgcg ctcggcaacc ggcgcgtatt
     2701 tgcgatcgcg acggtcgatc tcgagaaaac ctgtaatctt gcccattaaa gccagcgccc
     2761 ctgcatctct accgtcatcc tgaggtgcgc gcccttgcgc gcctcgaagg atgagccaca
     2821 aacacttccg cttgtcatcc ttcgaggctc gccgaagacg gctcgcacct caggatgacg
     2881 ccaattctct ttgcctcatt ctgagcagcg gcgtcttcgc cgcgtctcaa agcatgagac
     2941 tcgtattctg ccgtcatggc tcaagacggc gcaagtgcgc gtcctcgcca tgagcaaaaa
     3001 ttaagccccg atcgcgattt tcggctcggc gtcggcgttg gccttcaatt ccttcagcgc
     3061 gcggcggtat tccaccggca tcaccttgcg gaatttcggc aaggaagttt tccagttcgc
     3121 cagaatgtcg gcggcgcgct tggagcccgt caacttggcg tgacgcgtga tcaagatgtg
     3181 cagccgctcg acatcggact ccagcaggtc ctggaacacg tcgacccggc catgcgcctc
     3241 gagatcgccg gactgatgat aggtgttcgc gttgatcatc tcttccgaca gcaccggctc
     3301 gagctgcacc atcgccatgt tgcagagctt ttcgaagtcg ccggcctcgt cgagaacgta
     3361 agccacaccg cccgacatgc cggccgcgaa attgcgcccg gtcttgccaa gcaccacgac
     3421 aatgccgccg gtcatgtatt cgcagcaatg gtcgccggcg ccttcgacca cggccacggc
     3481 gcccgaatta cgcaccgcaa accgttcgcc ggcgatgccg cggaaatagc actcgccctc
     3541 gatcgcgccg tacatcacgg tgttgccgac gatgatggat tcctccggca cgatgccgga
     3601 attgcgcggc ggcttgacga tgatgcggcc gccggacagg cccttgccga catagtcgtt
     3661 gccttcgccc tcgagatcga aagtgatgcc gcgcgccagc caggcgccga acgcctggcc
     3721 cgcggtgccc ttgaagccca cgtgaatggt gtcgtgcggc agcccggcat ggccgtagat
     3781 tttggccacc gtccccgaca gcatcgcgcc ggcgctgcgg tcggtgttgt tgatcgccat
     3841 ctcgatcttg actggcgcgc cgcggtcgat cgcggcctgc gactgttcga tcagcctgcg
     3901 gtcgagcacg gcctccagat gatggttctg gccttgcgaa tgatagattt tctggcccgg
     3961 cagttcctgt tggcggacga acagttttga gaaatcgagc cccttggcct tccagtgcgc
     4021 caccagcgtg gattggtcga gcatctgggt ctggcccacc atctcgttga aggtgcggta
     4081 gccgagggct gccatgatct cgcgcacttc ctcggcgacg aagaagaaat agttgatgac
     4141 gtgctcgggc tggcccgtaa aacgcttgcg cagcactggg tcctgggtcg caacacctac
     4201 gggacaagtg ttgagatgac acttgcgcat catgatgcag ccggccgcga tcaggggtgc
     4261 ggtggcaaag ccgaattcgt cggcgccgag cagcgcgccg atcacgacgt cgcgcccggt
     4321 gcggaagccg ccatcgacct gcaccaagat gcggctgcgc agccgctcgc gcaccagcgt
     4381 ctggtgggtt tcggcgaggc cgatttccca cggggatcct gcgtgcttga tcgaggtcag
     4441 gggagacgcg ccggtaccgc cctcgaatcc tgcgatggtg acgtggtcgg cgcgcgcttt
     4501 ggcaacaccg gcggccaccg tgccgacgcc gatttcggag accagcttga ccgataccag
     4561 gccgtccgga ttgacgttct tcaggtcata gatcagctgc gccagatcct cgatcgaata
     4621 gatgtcgtga tgcggcggcg gcgaaatcag gccgacaccc ggcgtcgaat gccgcacccg
     4681 cgcgatcacg gcgtcgacct tgtggccggg caattgtccg ccttcgccgg gcttggcgcc
     4741 ctgcgccatc ttgatctgca tcatgtcggc gttgacgaga tattcggtgg tgacgccgaa
     4801 gcggcccgag gcaacctgct tgatcgccga gcgcatcgaa tcgccgttcg gcattggctt
     4861 gaagcgaccg ctctcctcgc cgccttcgcc ggtattggac ttgccgccga tccggttcat
     4921 cgcgatcgcg agcgtggtgt gcgcctcgcg cgagatcgac ccaaaactca tcgcgccggt
     4981 ggcgaaacgc ctgacgatgt ccttggcggg ctcgacctgc tcgagcggca ccggcttgcg
     5041 cttctcgtgc tcggcgggct tgatcttgaa caggccgcgc agggtcaaca gccgctcgga
     5101 ctgttcgttg aggattttcg cgaacgcctt gtagcgctcc agcgagttgc cgcgcacggc
     5161 atgctgcagc gtcgagaccg actcggcggt ccaggcgtgt tcctcgccgc gggtgcggta
     5221 ggcatattcg ccgccgacat cgagcgcggt cttgtacatc ggcgcatcgc cgaaggcgtc
     5281 ggtgtggcgg cgcgcggttt cctcggcgat ttcggctaag ccaacgcctt cgatctgggt
     5341 gtgggtgccg acgaaatatt tcgagacgaa atccgccttc aatccgacgg cgtcgaagat
     5401 ctgcgcgccg caataggact gataggtcga gatgcccatc ttcgacatca ccttcaacag
     5461 gcccttgccg atcgacttga tgtagcgctt gacgatctcg tagtcgtcga gcgccgccgg
     5521 cagccggtcc ttcatggcga tgatggtctc gaacgccaga taagggttaa tggcctccgc
     5581 cccgtagcct gcgagacatg caaaatgatg cacctcgcgc ggctcgccgg attcgacgac
     5641 cagcccgacc gaggtgcgca agccggtgcg gatcagatga tggtgcacgg cggcgcacgc
     5701 cagcagcgag gggatcggaa tccggtccga gccggccatg cggtcggaca ggatcacgat
     5761 gttgataccc tcgcggaccg cggcttccgc gcgcgcgcac aattcgtcga gcacctgctc
     5821 gagcccggcc gcgccgaaac cggcgtggaa ggtggtgtcc agcgtgcgtg acttgaaatg
     5881 ggtgtcggcg atctccgaga tcgaccggac cttttccaga tccgcgtcgg tcaggatcgg
     5941 ctggcgcact tcaaggcgct tggtgaggcc ggccatgccc tgcagatcga acaggttcgg
     6001 ccgcggcccg atgatcgaga ccagactcat gaccagttct tcgcggatcg gatcgatcgg
     6061 cgggttggtc acttgcgcga agttctgctt gaagtaggtg aacagcggct tcggcttgtc
     6121 cgacagcgcc gagatcggcg tgtcgttgcc catcgagccg ttggcctctt cgccggtcga
     6181 cgccatcggc gtcatcagga tggtgacgtc ttcctggcta tagccgaacg cctgctgccg
     6241 atccaatagc ggcaggttgg agcgcatgcc cttggtcggt gcgtccggca aatcctccag
     6301 cacgatctgg gtgcggtcga gccaatcgcg atagggatgg ctgttggcca gctgcgcctt
     6361 gatctcgtcg tcgggaatga gccggccctg ttcgaggtcg accagcagca tcttgccggg
     6421 ctgcagccgc cacttggtaa cgatatgctc ctcggggatc ttcagcaccc ccatctccga
     6481 cgccatcacg atgcgatcgt cgctggtgac gagatagcgc gccggccgca agccgttgcg
     6541 gtcgagcgtg gcgccgatct ggcggccgtc ggtaaacgcg atcgcggcgg ggccgtccca
     6601 cggctccatg atcgcggcgt ggtattcgta gaacgcgcgg cgcgtttcat ccatcaaggg
     6661 attgccggcc caggcttccg gaatcatcat catcacggcg tgcggcagcg agtagccgcc
     6721 ctgcaccagg aattcgaggc cgttgtcgaa gcaggcggta tcggactggc cctcatagga
     6781 aatcggccat agccggctga tatccttgcc gtagagttcc gaatgcaccg aggcctggcg
     6841 ggccgccatc cagttgacgt tgccgcgcag agtattgatt tcgccattat gcgcgatcat
     6901 ccggtacgga tgcgccagcg accaggccgg gaaggtgttg gtcgaaaacc gctgatgcac
     6961 cagcgccagc gcgctctcga aatcgggctc gctgagatcc ggatagtatt tgccgagctg
     7021 atcggcgagg aacatgccct tgtagatcac ggtgcggcac gacatcgacg ccggataata
     7081 gccggcgagg ccgcggtcgc ggcgcaaata gatcgcctgc gaaatcgact tgcgcaggat
     7141 gtagagccgg cgctcgaatt cgtcctcggt cttgatgtga tcgccgcgac cgatgaacac
     7201 ctgcatgttg gccggctcgg tcggcttgac ggtctcgccg agcgaggaat tgtcgaccgg
     7261 cacgtcgcgc cagccgagca gcatcaactg ctcggccttg atctggtcgg cgatgatgct
     7321 cttgatgact ttgcgccacg ccgtctcctt cggcatgaac agtgcaccga ccgcgtagtc
     7381 gcccggcttc ggcagctgga agccgatctc ggcggccttg cgcttgaaga aagtgtgcgg
     7441 aatctgcacc agaataccgg cgccgtcgcc ggcgcgcgga tcggcgccga ccgcgccgcg
     7501 atgctcgagg ttgcacagaa tgttgatcgc gtcgcagatg atctgatgcg acttcttgcc
     7561 cttgatgttg gcgatgaagc cgaccccgca ggaatccttt tccaggctcg gatcataaag
     7621 accctcggcg ggcgggcgcc agttgtgctc gcgtgcaggt tgatccggtt tcgacgccgg
     7681 ggccgccgac agcgcatccg tcacggtgtt ttcgcgctcg aactccgacc cgctcatctc
     7741 gtcctcccgc aagggcctaa aaaccgctgt cgttggcgca ccttgggcgt ttgcggtacc
     7801 caccgggcca ccgctcgtcc ttcgcgagcc gcaatttcat aaattcaggt ttgggctttc
     7861 ggcgtcccga cgaacggcct tgccttcacc ttccaggcgc ttgagcaccc gactttcgtt
     7921 gcaatccccg tgccgggttt gccaccgcaa ttcagacagt gatactgtcc taattgccga
     7981 ccatgtcaaa tttttttcta tcacacaagc gcgcgaaagt cgtcggttgc gtggcgggcc
     8041 cgtggcggac gtaaccacag tcgagtccaa tttgaggcag gcgcgccgcg atccggccca
     8101 aggactgccg gaatcggcgc agagtttgcc ggtggggaca tcacaggagc gttgggcaat
     8161 gaagtttttc ccagggttgg ccgtgtcggc cgcattggtt tgcgcggtcc cagccgcgga
     8221 tgcgcagacg ttggcgccat atgatgccgg aaaggggcgg ttcgtgtcgg atgtggacgg
     8281 tccctatgtc gacgtgccgg aagcgccgcc gcccgcgccg cgccattatg gttacggccg
     8341 ggatcgcggc tacgcgccgg atcgcggtta cgccccggcc taccgcgacg cgcccgctcg
     8401 tggctatgga ccggactatg gctacgctcc ggcgatgctg ccgccgcagg agatctacgc
     8461 gatcctgcgc gagaacggct tctcgccgct cggaatcccg cgtcagcgcg gttacgtcta
     8521 tgtgatcgcc gtgctcgatc ggggcggcga ggatggtcgc ctgacaatcg atggccgcaa
     8581 cggccggatc atccgtttcg tcccggcgtc ccagtggggt gaagcctacg atcgcatgag
     8641 ctttggccgt ggtcccgatc gaggttcaga tcgtagtcgg gatcatgcgc cgttctccgg
     8701 cgcggccgtg ggtgcgctgc cgacgccgat gcccatcaac gcgacgccgc gcccgccgac
     8761 gtcgcttccg tcggtcgcga tccgcggcgc cgttccggcc caggcacaga ggccggcggt
     8821 cgttgccacc aagccgccag cccagccggt ccagcaaacg gcggcgaagc cggcggaagc
     8881 accgaagcaa gccggcactg tcggcgaggc caagccggca gcgccgcaga tcaagccgag
     8941 ccaggagatg cctgcggtgc agggcctgga atagtaccgc aaacggcgcg tcactcagac
     9001 atccgctgga tcagcgtccc ggctcgccgt ggcgctgttt tcggatcatg ctttatttct
     9061 tctgacccgc ggcagccagg atcagatcgg caatctgctt cggatgtgac accagggaga
     9121 gatgacctgc ctccagctcc acggtggtcg cgttcatgcg ctttgccaga aaacgctcga
     9181 gatcgggatt gatggtctga tcgagttttg acaccgcgta ccatgagggt tttgagcgcc
     9241 acgctgcggc ggtggttcgt ccgccgaaca aggtggcggc cgttggttgc tgcacggcat
     9301 acagcacttc tgccgccttg ggctcgacgc cgttggcaaa atatttgagg aaagactctt
     9361 cggacagctt ggtaaaacca tcatgttcct ggacacccgc ccgcaccggt ccggtcggaa
     9421 atttggcgga gagggcgacg aaatcctccg cggcgtcggg ggctcgtgcg gcaacataga
     9481 ccagggaagt gaccttcggg tcgatcccgg tctcgctgat gacggtgccg ccccaggagt
     9541 gggcgaccag cacggtcggg ccatcctgca gcgccaatgc gcggcgcgtc gcggcgaccg
     9601 agtcttcgag cgaggtcagc ggattttgca cggcggtgac tttaagaccg gccgcctgca
     9661 gatatggaat gaccgcagcc cagctggagc cgtccgccca cgccccatgc accagcacga
     9721 cgttgcgcgc ctcggtgggc gcggacgttt gcgcgtgtgc gtcaaatccg ctgagcgttg
     9781 ccgcgagcgc ggcgacggtg agtgcaatca cgactcgggc tatcttcatc ataccaactc
     9841 catggccaaa acttccgtgc gcagcgttgc gcgtcataag tgtttttcgg acgatggggt
     9901 cgcaacgtta cgggggttca cagctctgtg aattttcgaa agatcccgcg tgcagtgact
     9961 ggagcgggac cggatacgaa cgaggggggt tagagaaact acatcgtcat tccggggcgc
    10021 gccaacgggt ccgcgcaaag cgcggcccga tgacaggctc cgcgcgagcc cggaatccat
    10081 aaccacgaca gggagtatgg attccgggcc tgcgcataca agtcggctgt agccgacttg
    10141 gacaatgata ttgccgaact cgggtaaacc cgagttcggt tggcgcatcc acgatgtgca
    10201 cttgcacatc ggggaatgac gacggaaaca aaaacgcccc ggtctcccgg ggcgtttcgt
    10261 attcagaact cgttcagtac ctggcgcgaa accaacccgc gttacgcggc ggctttctcg
    10321 gccgagttgt cggcgacctg cggagcgacg gcgctcgtgg tcgtgatcgg aatgctgcgg
    10381 ggtttcttgg cttcgggaat ctcgcgaacg agatcgacgt gaagcaggcc
//
LOCUS       DASWGM010000074         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1529066,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000074 DASWGM010000000
VERSION     DASWGM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529066"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGA15_02415"
     CDS             complement(<1..213)
                     /locus_tag="VGA15_02415"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473095.1"
                     /note="Conjugates Arg from its aminoacyl-tRNA to the
                     N-termini of proteins containing an N-terminal aspartate
                     or glutamate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX9586584.1"
                     /translation="MTQHSRDTPQFYLTAPSPCPYLPGRHERKVFTHLVGDKAGDLND
                     LLTHGGFRRSQSIAYRPACDQCRACVS"
     gene            complement(328..882)
                     /locus_tag="VGA15_02420"
     CDS             complement(328..882)
                     /locus_tag="VGA15_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX9586585.1"
                     /translation="MSDSGSGYSSGGAWRSGGANYQPHAFDPLSQPELFRGVLTRRVF
                     AFLIDLVVLAIPVILAYVFIAVFGLITLGLGWLLFWLVWPASVIWAVIYYGASLGGPH
                     SATMGMRVMDLELRTWYGAPSYFVLGATHAVLFWVSISFLTPLVLLIGLFNGRRRLLH
                     DIVLGTVVINSSVRTQVAQPARTF"
     gene            complement(1080..2141)
                     /gene="hemB"
                     /locus_tag="VGA15_02425"
     CDS             complement(1080..2141)
                     /gene="hemB"
                     /locus_tag="VGA15_02425"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX9586586.1"
                     /translation="MAIKFGRPIEMRDAPRQKTGLAAPTLDLVVRPRRNRKAEWARRL
                     VRENVLTSDDLIWPLFVIDGDNKRAGVASMPGVERLSVDQAVRDAERAMKLNIPCIAL
                     FPYTEPSLRDEHGSEALNSDNLVCKSVRAIKQEFPGLGVLCDVALDPFTSHGHDGLIE
                     DGRILNDETVAVLVRQALVQAEAGCDIIAPSDMMDGRVGAIREALDAAGFLDVQIMAY
                     AAKYASAFYGPFRDAIGSAKTLTGDKRTYQMDSANSDEALREVELDIAEGADMVMVKP
                     GMPYLDIVRRVKDTFAMPTFVYQVSGEYAMIAAASGNGWIDGERAMMESLLGFKRAGA
                     DGILTYFARQAAEKLKMEG"
     gene            2375..2836
                     /locus_tag="VGA15_02430"
     CDS             2375..2836
                     /locus_tag="VGA15_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6163 family protein"
                     /protein_id="HEX9586587.1"
                     /translation="MSQAASRDHSHDNAMSVAAISSERIETDENVWTRRLVFFLRIMA
                     VVSVAKGLYHWAQVTGFIGGEEDAFENQAMAWQTATVYFAVIELVAAVGLWLATPWGA
                     VVWLTTVVSMAVIELMFPGIYGGSLTVVGLEAVMLAAYLVLAWMAARERPP"
     gene            3320..3835
                     /locus_tag="VGA15_02435"
     CDS             3320..3835
                     /locus_tag="VGA15_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9586588.1"
                     /translation="MMKAVATAVETAERTPGQAPVQPLYLEALTLVERLHRRLLDVIK
                     DEFDRRGRADINSVQALLLYNIGDKELTAGELRTRGYYLGSNVSYNLKKLVELGFLDH
                     QRSRVDRRSVRIRLTAQGQEIRRIVDALYQKHVKTVEQVGGISNEEFATLNKSLHRLE
                     RFWTDQILYRL"
BASE COUNT          807 a         1236 c         1222 g          774 t
ORIGIN      
        1 ggaaacgcag gcgcggcact ggtcgcaggc cgggcggtag gcgatcgact ggctgcgccg
       61 gaacccgcca tgggtgagca ggtcgttgag atcgccggcc ttgtcgccga ccagatgcgt
      121 gaacaccttg cgttcatgcc gtcctggcag ataggggcag ggcgagggcg ccgtgaggta
      181 gaattgcggg gtatcacgcg agtgctgggt cacgggatct cgtcagcctc cagaaagcga
      241 agcgggccag catcgcgccg cggaggctga ccgtcaatca gtttacccag gaacctttaa
      301 gcggacggtt aagcggttta cctgtcttca gaacgttcgc gccggttgcg ccacctgggt
      361 ccggaccgag ctgttgatca ccacggttcc caggacgata tcatgcagca gccgccggcg
      421 gccgttgaac aggccgatca gcagaaccag cggcgtcagg aacgatatcg acacccagaa
      481 cagcaccgca tgtgtggcgc cgaggacaaa atagctcggc gcgccgtacc aggtgcgcag
      541 ctccaagtcc atcacccgca tgcccatggt agccgaatgc ggcccgccca gcgatgcgcc
      601 gtaatagatc acggcccaga tcaccgaggc cggccacacc agccagaaca acagccagcc
      661 cagtcccagc gtgatcaggc cgaacacggc aatgaacacg taagccagaa tgaccgggat
      721 cgcgagcacc acgaggtcga tcagaaacgc aaacacccgg cgcgtcagca cgccgcgaaa
      781 caattccggc tgcgagagcg gatcgaaggc atgcggctga taattcgcac cgccgctgcg
      841 ccaggcgccg ccgctgctgt aacccgaacc gctgtcagac atgatccgtc ctccgcaact
      901 gtgccgtcgc cccgcgagac atgggaatgg gtagctccgg tcgcaagggg aaatactccg
      961 tcattgcgag ccagcgggtc gcgcgaatgc gcgcccgatg ataaactccg cgtgcccacc
     1021 atcttcagaa aaacggtggg cacggcgcaa gcgcgccttt acccacccta cgaagtgcct
     1081 cagccctcca tcttcagctt ttccgccgcc tgccgcgcga agtaggtcaa aatgccgtcg
     1141 gcgccggcgc gcttgaaccc caagaggctt tccatcatcg cccgctcgcc gtcgatccag
     1201 ccattgccgc tggctgccgc gatcatcgcg tattcgccgg acacctgata gacgaaggtc
     1261 ggcatcgcaa aagtgtcttt gacgcgccgc acgatgtcga gatacggcat gcccggtttg
     1321 accatcacca tgtcggcgcc ttcggcgatg tcgagttcga cctcgcgcag cgcctcgtcg
     1381 gaattggcgc tgtccatctg ataggtgcgc ttgtcgccgg tcagcgtctt cgccgagccg
     1441 atggcgtcgc ggaacgggcc gtagaaagcg gaggcatatt ttgccgcata ggccatgatc
     1501 tgcacgtcga gaaatccggc agcatcgagc gcctcgcgga tggcgccgac gcggccgtcc
     1561 atcatgtcgg aaggtgcgat gatgtcgcag ccggcttcgg cctgcaccag cgcttgccgc
     1621 accagcaccg cgacggtctc gtcgttgagg atcctgccgt cctcgatcag gccgtcatgg
     1681 ccatggctgg tgaagggatc cagcgcgacg tcgcagagca cccccaaacc gggaaattcc
     1741 tgcttgatcg cgcgcaccga tttgcacacc agattgtctg aattaagcgc ctcggagccg
     1801 tgctcgtcgc gcaggctggg ctcggtatag ggaaacagcg cgatgcaggg gatattcagc
     1861 ttcatcgcgc gctcggcgtc gcgcaccgcc tgatcgacgc tgagccgctc gacgccgggc
     1921 atcgaggcaa cgccggcacg cttgttgtcg ccgtcgatca cgaacagcgg ccagatcaga
     1981 tcatctgatg tcaggacgtt ttcccgcacc agccggcgtg cccattccgc cttgcggttg
     2041 cggcgcgggc gcaccaccag atcgagggtc ggggcggcga ggccggtctt ttgccgcggc
     2101 gcgtcgcgca tttcgatcgg acgcccgaat ttgatcgcca tgtgatggtc tctcctgaag
     2161 gcggtgagat gtggaggatt tgaattattc caatcctagc actttgtatt gcttccgtca
     2221 ccgcggcctt gacctgccgc aagtaccctt acgcaagtat ggctaaactg ggaaaaatgt
     2281 cgcaacgcac cgcaatggcc cgattgattt tgctgcgcca agaggccatg agtgcccgat
     2341 gaaggcacgt tcgcgatcga gagccccgac cccgatgtca caagccgcgt cgcgcgacca
     2401 ctcgcacgac aatgccatgt cggtggcggc gatctcgtcg gagcggatcg agaccgacga
     2461 aaatgtctgg acccggcggc tggtgttttt cctgcgcatc atggccgtcg tctcggtcgc
     2521 caagggcctc taccactggg cgcaggtgac cggctttatc ggcggcgaag aagacgcatt
     2581 cgaaaatcag gcgatggcat ggcagaccgc cacggtttat ttcgcggtga tcgaactcgt
     2641 cgccgcggtc ggattgtggc tggcgacgcc gtggggcgcg gtggtgtggc tgaccaccgt
     2701 ggtgtcgatg gcggtgatcg agctgatgtt tccgggaatc tatggtggca gcctgacggt
     2761 ggtgggtctg gaagccgtga tgctggcggc gtatctcgtg ctcgcatgga tggccgcgcg
     2821 cgaacgcccg ccatagggta gcgaaattcc aggcgcccga tccgctaaag tgtaagcggc
     2881 ttagcgatga gatcatgcgc cattttttca gagcgcgatc ggacgcaaaa ccggattcca
     2941 catttttctg atcgcgatct cttgcgtgcc ggaattggcg cgccttcgca taaaattttc
     3001 ggggtttttg atggtaactt ttcagtcacc ctaacgcagt ggtcacacct gttacgctta
     3061 agtttcaaat tcgagcgatg ctgttccctt ctgcgacaga gtgcgcatac gaagcgtgaa
     3121 cagggccctc acaccgtcaa tgaaacgttg cgaaagccct tgattggcgg gcttaatccg
     3181 cgattcactg tcttaaattc atgatctctt tattctctta tttaagcgga tcttcaaatc
     3241 gcccccttaa gttgaggcta tcaggcggga caaaagtttc gtcgaataag tcgataaaaa
     3301 cgacaacagg ggaagtgtca tgatgaaagc cgttgcaacg gcggtggaaa ccgctgaacg
     3361 cacgccgggc caggctccgg tgcagccgct ctatctcgaa gcgttgacgt tggtggagcg
     3421 gctgcaccgc cgcctgctcg atgtcatcaa ggatgaattc gatcgccgcg gccgcgccga
     3481 catcaattcg gtgcaggcgc tgctgctcta taacatcggc gacaaggaat tgaccgcggg
     3541 cgaactgcgc acgcgcggct attacctcgg ctccaacgtc tcctacaatc tgaagaagct
     3601 ggtcgaactc ggcttcctcg accatcagcg ttcgcgcgtc gatcgccgtt cggtccgtat
     3661 tcgtctgacc gcgcagggcc aggaaatccg ccgcatcgtc gatgcgctct atcagaagca
     3721 cgtcaagacc gtggagcagg tcggcggcat ctccaacgag gagttcgcga ccctgaacaa
     3781 gtcgctgcac cggctcgagc ggttctggac cgaccagatc ctgtatcgtc tctgagactt
     3841 tttttcgaac gcagtcaaag gccaagccgg catctccaaa agaccggcag ccacgagacg
     3901 aaaagaccac ttccccaagc gcatcggccc ggttttcccc cggaccggtg cgttttgttg
     3961 tgtgcactgc aaaaaatcag gcttcccgaa ttggaactta tgcccgcttt cgtgctttag
     4021 tccggatcgg aggactgct
//
LOCUS       DASWGM010000075         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_143057,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000075 DASWGM010000000
VERSION     DASWGM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143057"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="VGA15_02440"
     CDS             complement(<1..92)
                     /locus_tag="VGA15_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX9586589.1"
                     /translation="MLSRTAENLYWLARYVERAEYLARTIEATLR"
     gene            complement(144..1562)
                     /locus_tag="VGA15_02445"
     CDS             complement(144..1562)
                     /locus_tag="VGA15_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX9586590.1"
                     /translation="MAVAFDEMNGPGGDLRPAYQELSRWLKETPPDALEYRRQEAELL
                     FRRIGITFAVYGDAEAQERLIPFDVIPRIMSGKEWAILEKGLKQRVRALNMFLRDIYH
                     SRDILRANIIPDDLIFQNPVFRPEMNGQDVPHDVYVHIAGIDIVRVNPEDFYVLEDNA
                     RTPSGVSYMLENREIMMRLFPDLFARHRVAPVEKYPDELLSALRSVAPLSAAAEPTVA
                     LMTPGVYNSAYYEHSFLADKLGVELVEGRDLIVKNDEVFMRTTEGLKRVDVIYRRVDD
                     DFLDPLTFRPDSALGVPGLMSAYAAGNITLANAVGTGIADDKAVYSYMPEIVKFYLGE
                     EPILKNVPTWRCREAKDLAYVLDNLSELVVKEVHGSGGYGMLIGPAATKATIEAFRDK
                     LKREPEGFIAQPTLALSTCPTCTSSGLAPRHVDLRPFVLTGRDRITIVPGGLTRVALK
                     EGSLVVNSSQGGGTKDTWILDE"
     gene            1779..>1900
                     /locus_tag="VGA15_02450"
     CDS             1779..>1900
                     /locus_tag="VGA15_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX9586591.1"
                     /translation="MSDIVTAGILVIGDEILSGRTKDKNIGFIAEYLTNIGIDL"
BASE COUNT          366 a          556 c          612 g          366 t
ORIGIN      
        1 cgcagcgtcg cctcgatggt gcgcgcgaga tattcggcgc gttcgacata gcgggccagc
       61 cagtacaggt tttcagcggt gcgcgacagc atgcagcgat gttgctctct tgatgcctgg
      121 atctactcgt ccagaatcca aatctactcg tccagaatcc acgtgtcctt ggtgccgccg
      181 ccctggcttg aattcaccac cagcgagcct tccttcagcg ccacccgcgt cagcccgccc
      241 ggcacgatgg tgatgcgatc gcgtccggtc agcacgaacg gccgcaggtc gacgtggcgc
      301 ggcgcgaggc cggatgaggt gcaggttggg caggtcgaca gcgccagcgt cggctgggcg
      361 atgaacccct cgggttcgcg cttgagcttg tcgcgaaacg cctcgatcgt cgccttggtc
      421 gcggccgggc cgatcagcat gccgtagccg ccggagccgt gcacttcctt gaccaccagt
      481 tcgctgagat tatccagaac gtaagccaga tcctttgcct cgcgacagcg ccaggtcggc
      541 acgttcttca ggatcggttc ctcgccgaga tagaatttca cgatctcggg catgtaggag
      601 tagaccgcct tgtcgtcggc gatgccggtt ccgacggcat tggccaatgt gatgttgccg
      661 gccgcgtagg ccgacatcag cccgggcacg ccgagggctg aatccgggcg gaaggtgagg
      721 ggatcgagga agtcgtcgtc gacgcggcgg tagatcacat cgacgcgctt caagccctcg
      781 gtggtgcgca tgaacacctc atcgttcttg acgatgagat cgcggccctc gaccagttcg
      841 acgccgagct tgtcggccag gaatgaatgc tcgtaatagg cggaattgta gacgccgggc
      901 gtcatcagcg ccaccgtcgg ctcggccgcg gcgctgagcg gcgccaccga ccgcagcgcc
      961 gacaatagct catcggggta cttttccact ggcgcgacgc ggtggcgggc aaacaggtcc
     1021 ggaaacagcc gcatcatgat ttcgcggttt tccagcatgt aggagacgcc ggagggcgtg
     1081 cgtgcattgt cctccagcac gtaaaaatct tccgggttga cgcggacaat gtcgatgccg
     1141 gcgatgtgga catagacatc gtgcggcacg tcctggccgt tcatctcggg gcggaacacc
     1201 ggattctgga agatcaggtc gtcgggaatg atattggcgc gcaggatatc gcggctgtga
     1261 tagatgtcgc gcaggaacat gttgagcgcg cgcacccgct gcttcaggcc cttttcgagg
     1321 atcgcccatt ccttgcccga catgatccgc gggatcacgt cgaatggaat caaccgttcc
     1381 tgggcttcgg cgtcgccata gacggcaaag gtgatgccga tccggcggaa cagcaactcc
     1441 gcttcctgac ggcgatattc cagcgcatcc ggtggcgtct ccttcagcca gcgggagagc
     1501 tcctggtagg ccggacggag gtcgccgccg ggcccgttca tttcgtcaaa ggcaactgcc
     1561 atatatcccg actgttcctc aaaggccatg cgggacagtg catgactgaa agggatggta
     1621 gcaaggcccg ggccagcgcg atatgcattc acgcggagca tttgctatgg atgattggga
     1681 agttgcccga aaaaacggct gccaggtgct tatttcggca gcaaagcccc atgacttcaa
     1741 ggtgttgcgg gggtcatgat gcggggcgag gagaggtcat gagcgatatc gtcacggcgg
     1801 gaattctggt gattggagac gagatcctgt ccgggcggac caaggacaag aacatcggct
     1861 ttatcgcgga atacctgacc aatatcggga tcgacctcaa
//
LOCUS       DASWGM010000076         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_6736, whole
            genome shotgun sequence.
ACCESSION   DASWGM010000076 DASWGM010000000
VERSION     DASWGM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6736"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..436
                     /gene="rplT"
                     /locus_tag="VGA15_02455"
     CDS             77..436
                     /gene="rplT"
                     /locus_tag="VGA15_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX9586592.1"
                     /translation="MSRVKRGVTSHAKHKKVYKAAKGFYGRRKNTIRTAKAAVEKAAQ
                     YAFRDRKRKKRTFRALWIQRLNAAVRPLGMTYSVFINGLAKSGITVDRKVLSDLAIHE
                     PAAFQAIAEKAKAALAA"
     gene            614..1720
                     /gene="pheS"
                     /locus_tag="VGA15_02460"
     CDS             614..1720
                     /gene="pheS"
                     /locus_tag="VGA15_02460"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX9586593.1"
                     /translation="MTDLVQLESQILSQIGAASDEAALEAVRVATLGKKGSISALLST
                     LGKMSPDERKTQGAAINLAKDKVTQALTSRRDVLKSAALDARLASETLDVTLPLRETP
                     AEQGRIHPLSQVMDELTTIFADMGFAIAEGPDIETDDYNFTKLNFPEGHPAREMHDTF
                     FFQPKEDPGSEPGAGGSRMLLRTHTSPVQVRTMLTQKPPIRIVCPGRTYRIDSDATHT
                     PQFHQVEGLVIDKGSHLGRLKWILHEFCKAFFEVDHINMRFRPSFFPFTEPSLEVDIQ
                     CRRDKGEIRFGEGEDWLEILGCGMVHPNVLRACGIDPDVYQGFAWGMGIDRIAMLKYG
                     MSDLRQLFENDVRWLNHYGFKPLDVPTLAGGLSS"
     gene            1717..2133
                     /locus_tag="VGA15_02465"
     CDS             1717..2133
                     /locus_tag="VGA15_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEX9586594.1"
                     /translation="MSDPIIPEAYRTLRSFAFGDGPDLADELLELVLKGVKTATCSTE
                     DEPNTSTPGECWIVLDGRGNPGCVIESTEVTYRRYGDVDAGFAHDEGEGDRSLAYWRS
                     AHRSYFGRLGKFSEDMMLMCERFRLVEIFAESRVAS"
     gene            2130..>2541
                     /locus_tag="VGA15_02470"
     CDS             2130..>2541
                     /locus_tag="VGA15_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX9586595.1"
                     /translation="MKFTLSWLKDHLDTDEPVEKLADKLTMIGLEVEHIEDKAKALAP
                     FTIARVISAEQHPNADRLRVCMVDTGDGGAPVQVVCGAPNARAGLVGVFSAPGTFIPG
                     KNITLGVGTIRGVESRGMLCSAAELQISEDHDGIM"
BASE COUNT          495 a          819 c          766 g          461 t
ORIGIN      
        1 ggcacgccgt ccatcacagc cgagccgcgt ccgcgcggcg atccgtcacc gatttttcat
       61 atgaaggaga tccgtcatgt ctcgcgtcaa acgcggtgtg acctctcacg ccaagcacaa
      121 gaaagtctac aaggccgcca agggctttta cggccgccgc aagaacacca tccgcaccgc
      181 aaaggccgcg gtcgagaagg ccgcgcaata cgccttccgc gaccgcaagc gcaagaagcg
      241 caccttccgc gcgctgtgga ttcagcgcct caatgccgcc gtgcgtccgc tcggcatgac
      301 ttacagcgtc tttatcaacg gccttgccaa gtcgggcatc acggtcgacc gcaaggtgct
      361 gtcggatctc gccatccacg agccggcggc gttccaggcg atcgctgaaa aggccaaggc
      421 cgcgctggcg gcttaagttc gcaaatcgct gcggcgtcct gcaactccgg tgtcatgcgc
      481 cgcgaaagcg gggcatccag taaccaccga ccgacatttt tgaccccggg ggttactgga
      541 tcatccgctt tcgcggatgc tgacggcctt gcgtgccgga ctgcgagcgc gctccccaca
      601 ggtgaccgtc gccatgaccg accttgttca actcgagtcc caaatcctca gccagatcgg
      661 cgcagcgagc gacgaagccg cgctggaggc cgtgcgcgtt gcaaccctcg gcaagaaggg
      721 ttcgatctcc gcgctgcttt cgacgctcgg caagatgtcg ccggacgaac gcaagacgca
      781 aggtgctgcg atcaatctgg ccaaggacaa ggttacccag gcgctgacct cgcgccgcga
      841 cgttttaaag tccgcagccc tcgacgcgcg gctggcctcc gaaacgctcg acgtcacgct
      901 gccgctacgt gagactccgg ccgagcaggg acgaatccat ccgctcagcc aggtgatgga
      961 cgaactcacg acgatcttcg ccgacatggg atttgccatc gccgaaggcc cggatatcga
     1021 aaccgacgac tacaacttca caaaactgaa ttttccggaa ggccatccgg cgcgggaaat
     1081 gcacgatacg tttttctttc agccaaaaga agaccccggg tcggagcccg gggcaggcgg
     1141 ctcgcgcatg ctgttgcgca cccacacttc gccggtgcag gtgcgcacca tgctcacaca
     1201 aaagccgccg atccgcatcg tctgtccggg ccgcacctat cgcatcgatt ccgacgccac
     1261 gcacacgccg cagtttcacc aggtcgaggg tctcgtcatc gacaagggtt cgcatctcgg
     1321 ccgcctcaaa tggatcctgc acgaattctg caaggcgttc ttcgaggtcg atcacatcaa
     1381 catgcgattc cggccgtcgt tctttccgtt caccgagccg tcgctggagg tcgatatcca
     1441 gtgccggcgc gacaagggcg aaatccgctt cggcgagggc gaggactggc tggagattct
     1501 cggctgcggt atggtgcatc cgaatgtgct gcgggcctgc ggcatcgacc ccgacgtcta
     1561 tcagggcttt gcctggggca tgggcatcga ccgcatcgcg atgctgaaat acggcatgtc
     1621 cgacctgcgg cagctgttcg agaacgacgt gcgctggctc aatcattacg gcttcaagcc
     1681 gctcgacgtc ccgacgctgg cgggaggatt gagttcgtga gtgatccgat tattccggaa
     1741 gcctatcgga cgctgcggtc tttcgcgttc ggcgacggtc ctgatctggc cgacgaactg
     1801 ctcgaacttg tcctcaaggg cgtcaagacc gccacctgca gcaccgagga tgagccgaac
     1861 acgtcgacgc cgggcgaatg ctggatcgtg ctcgacgggc gcggcaatcc cggctgcgtc
     1921 atcgaatcga ccgaggtcac ctatcgccgt tacggcgatg tcgatgccgg cttcgcgcac
     1981 gacgagggcg aaggcgatcg cagtctcgcc tattggcgat cagcgcatcg ctcgtatttt
     2041 ggacggctgg gaaaattcag cgaagacatg atgctgatgt gcgagcgttt tcgcctggtc
     2101 gagattttcg ccgagagcag ggtagcatca tgaaattcac gctctcctgg ctgaaggacc
     2161 atctcgacac cgatgagccc gttgaaaagc tcgccgacaa gctcaccatg atcgggcttg
     2221 aggtcgagca catcgaggac aaggccaagg cgctcgcgcc tttcaccatt gcgcgggtga
     2281 tctccgccga gcagcatccc aatgcggacc gtctgcgggt ctgcatggtc gataccggcg
     2341 acggcggcgc gccggtgcaa gtcgtgtgcg gcgcgccaaa cgcgcgcgct ggcctcgtcg
     2401 gcgtgttttc agcgcccggc actttcattc ccggcaagaa catcacgctc ggcgtcggca
     2461 ccatccgcgg tgtcgaaagc cgcggcatgc tctgttcggc agccgaactg caaatctcgg
     2521 aggatcacga cggcatcatg g
//
LOCUS       DASWGM010000077         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2363375,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000077 DASWGM010000000
VERSION     DASWGM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363375"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..831)
                     /locus_tag="VGA15_02475"
     CDS             complement(28..831)
                     /locus_tag="VGA15_02475"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9586596.1"
                     /translation="MFKRMHVALLAGASCFCAGLHQAAAIEAAPLLEPTVIYAEQPAQ
                     RPLAMPTAYAGRPNMGGGFIEFLFGDGQSQGERYQQQPGYQQQPSYQPQRSLLPQFEP
                     RQGLRRQEDAGEPDQAAFDPKFEKQLVDYHGREGAGTIVIDTPNKFLFLVQGDGTALR
                     YGIGVGRPGFTWSGVKQISAKKEWPAWTPPPEMLARRPDLPRHMEGGPQNPLGARAMY
                     LGSSLYRIHGSNEPWTIGTNVSSGCIRMRNEDVIDLYGRVNVGARVVVI"
     gene            1127..1897
                     /locus_tag="VGA15_02480"
     CDS             1127..1897
                     /locus_tag="VGA15_02480"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEX9586597.1"
                     /translation="MPVHRRRFRIEQAVVGDMPMPAAADGDVGPIHHEIMAELRAIRA
                     QMASPSRSVTTETIGATVNREVAEALALLETYRAQIEQCEKLKIELDLIHDAITRTKR
                     EIATLHGKSFNGEEMAKVNGELGAVVGGTEEATQQILEAAEAIDNAAAALSKVNTPDQ
                     QKLLSEEIQERVVSIFEACNFQDLTGQRISKVMTTMKFIENHIVVMMDIWGGVDAIKA
                     HAPAIIDERKGDDRLLNGPKLDGDAGHASQNDIDALFD"
     gene            complement(2098..2610)
                     /locus_tag="VGA15_02485"
     CDS             complement(2098..2610)
                     /locus_tag="VGA15_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586598.1"
                     /translation="MVRSCNARAVANLGIASALALFLGACGGISLPSFSSSSAAPEPG
                     VEPEMPASIRADEIVGRWGLASFQNPNDRARTEVQAKAQCKQPYVIGAGTSGGVVMHL
                     ADQATPQELRLKGTSSGKNYIGPAGPAGGEQDREIVSFDGRVLVTRFIDKDAATRYGN
                     MVYVRCGARA"
BASE COUNT          597 a          877 c          871 g          554 t
ORIGIN      
        1 gcggccgttt tggttttcag gaaaaaatca gatgacgacg acccgcgcgc cgacattgac
       61 ccggccatac aggtcgatca cgtcctcatt gcgcatccgg atgcagccgg atgacacgtt
      121 ggtgccgatg gtccagggct cgttggaacc gtggatgcga tagagcgacg atcccagata
      181 catcgcgcgg gcaccgagcg gattttgcgg gccgccttcc atgtgccgcg gcagatcggg
      241 gcgccgcgcc agcatttccg gcggcggcgt ccaggccggc cattccttct tcgccgagat
      301 ctgcttgacg cccgaccagg tgaagccggg gcggccgacg ccgatgccgt agcgcagcgc
      361 cgtgccgtcg ccctgaacca ggaacagaaa cttgttgggc gtgtcgatca cgatcgtgcc
      421 ggcgccttcc ctgccgtgat aatcaaccag ttgcttctcg aatttcggat cgaacgcagc
      481 ctgatcgggc tcgcccgcat cttcctgccg gcgcaaccct tgccgcggct cgaattgcgg
      541 caacagcgaa cgctgcggtt ggtaggatgg ctgttgttga tagccgggct gctgctgata
      601 gcgctcacct tgcgactgtc cgtcgccgaa caggaactcg atgaaaccgc cgcccatatt
      661 ggggcgcccg gcataagcgg ttggcatcgc cagaggccgc tgcgcgggtt gctcggcgta
      721 aatcacggtg ggttccagaa gtggggcggc ttcgatcgcc gcggcttgat gaagtccggc
      781 acaaaaacaa gatgctccgg cgaggagcgc aacatgcatt ctcttgaaca tcgacgtact
      841 ctgcactgtt tcgtcttggc gagcgccggg tcagcgccct tgcacgccac caattagaat
      901 caaaaccgca tcggtttggt aaacagaaac ggcctttgga ttcaccacgt cgccaattcc
      961 gcgcggtttc atccgctagc gtttattttc ggtgaacggt gggagcatat ggttaatcgt
     1021 tggtgtgcga gtacaagcca acttgcgaca agattcttcc ggcgtgaacc ccatgttaaa
     1081 tcgcctctgt gtaaaacgtg acgagtgaag gttaggaaaa aatctcatgc cggttcatcg
     1141 cagacgtttt cgtatcgaac aagcagtcgt cggcgacatg ccgatgccgg cagccgctga
     1201 cggcgatgtc ggtcccattc atcacgagat catggccgag cttcgcgcca tccgcgccca
     1261 gatggccagc cccagcagga gcgtgacaac ggagaccatc ggcgcgacgg tcaaccgcga
     1321 ggtcgctgaa gcgcttgcgc tgctggaaac ctaccgggcc cagatcgagc agtgcgagaa
     1381 gctcaagatc gaactcgacc ttattcacga tgccatcacc cgcaccaagc gggaaatcgc
     1441 gacgcttcac ggcaagagct tcaacggcga ggaaatggcc aaagtcaacg gcgagctcgg
     1501 tgccgtggtc ggcggtactg aagaagccac gcagcaaatt ctggaagccg cggaggcgat
     1561 cgataatgcg gcggccgcgc tttccaaggt gaacaccccg gaccagcaaa agctgctcag
     1621 cgaggaaatc caggaacgcg tcgtttccat cttcgaggcc tgcaatttcc aggatctcac
     1681 cggtcagcgc atcagcaagg tgatgaccac gatgaaattc atcgaaaacc atatcgtggt
     1741 gatgatggat atctggggtg gcgtcgatgc gatcaaggca cacgcgcctg ccattatcga
     1801 cgaacggaaa ggcgacgacc ggttgctgaa cggtccgaag ctggacggcg acgccggtca
     1861 tgcctcgcag aacgacatcg acgcattgtt tgactgagct tgatggatcc ttgcgccggg
     1921 tggcagcacc ctgcgaccgg agcgccagcg ttggattcaa ctccagttga atcagactct
     1981 ggcgctatct ttttggtgta gcctgaccgt tccggaaaac cggattccgc cgcgccggat
     2041 aaatacttcg ggcgtcagac cgcgggtttt agcgcgggcc gccggattgg cgatggatca
     2101 ggcgcgcgcc ccgcagcgga catagaccat gttgccatac cgggtggcgg catccttgtc
     2161 gatgaagcgg gtgaccagaa cgcggccgtc gaaggaaaca atctcgcggt cctgctcgcc
     2221 gccggccggt ccggccggac cgatataatt cttgccgctc gaggtgccct tgagccgcag
     2281 ttcctgcggc gtcgcctggt cagccagatg catgaccacg ccgcccgagg tgccggcacc
     2341 gatgacataa ggctgcttgc actgcgcctt ggcctgaact tcggtgcggg cgcggtcatt
     2401 gggattctgg aacgatgcga gaccccagcg cccgacaatc tcgtccgcgc ggatcgacgc
     2461 cggcatctcg ggctctacgc ccggctccgg agccgccgaa gaggatgaaa atgatggcag
     2521 gcttatgcca ccgcacgccc ccaggaataa cgctagcgcc gaggcgatcc ccagattggc
     2581 aaccgcacgc gcgttacatg acctgaccat aggtttcccc tcaagcaaat ttgtggccgc
     2641 acccgtcccg cagccaagcg taaaacctca gtcggagcaa tgatgtccag tcaattacgc
     2701 tggcgctccg agttagtttc cgtgaaagca tcctatattg gtctcaccag aggcttaacc
     2761 cccttgcttg acaggatcgg cgtcaagcca tattcccggg cgcacaatta gcactccgag
     2821 agcgcgattg ctaaggatgc gagctgtttt gccccttggc ggtgtcgaat acgcccgtat
     2881 tccgatccac tttcgatcg
//
LOCUS       DASWGM010000078         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_837208,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000078 DASWGM010000000
VERSION     DASWGM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837208"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VGA15_02490"
     CDS             complement(<1..291)
                     /locus_tag="VGA15_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586599.1"
                     /translation="MRVTDASGRGPQITAEEAAELQRAQERMIARHKLIEGMIRNNEM
                     QLKNESARGGAEIELECARRDVAQGDAGAGAQAELERATARLQALQEEHQRLV"
     gene            complement(356..1399)
                     /locus_tag="VGA15_02495"
     CDS             complement(356..1399)
                     /locus_tag="VGA15_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX9586600.1"
                     /translation="MKAAVLHAFDEKLTAKEFVKYEDVTDPKISRPMDVIVRVGGAGV
                     CRTDLHIVEGIWRSKVDVKLPYIMGHENAGWVEAIGPGVEGVKVGDSVICHPLVTSGH
                     CLACRRGNDMHALDSSFPGINANGGYAQYLLTGQRALIKLPKSLAPKDVAPYTDAGLT
                     AYRAAKKASRHLLPGEYVAVIGAGGLGHIGIQVLAALCAAEIIVIDRADKSLDLAKEC
                     GAHHLVKADGNEVEAVLALTGGRGAEAVIDFVGEGDAIAKGLSMTANGGYYYIVGYGG
                     KIELPTIDMITTEKTIVGNLVGTYPELVELMALADRGLVNLHTKEYKLSQANDALHDL
                     HHGHIHGRAVLIP"
     gene            1739..>2260
                     /locus_tag="VGA15_02500"
     CDS             1739..>2260
                     /locus_tag="VGA15_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9586601.1"
                     /translation="MMLPAGRRVENAWTTLEQRGAPPAELLSADIYDSWMRCISFGLD
                     TLRPPSPQFVSPAILRQEQQRCSLVRGLALAEMHTLHQQIAGSNFMIAFANADGLLLD
                     IISDPSFSDASNAASIRPGTVWTESICGTNGLGTAAHLKRAIVVHGREHFFSRYNNLT
                     CVAAPIFAPDGELA"
BASE COUNT          419 a          689 c          645 g          507 t
ORIGIN      
        1 gacgagacgc tgatgttcct cctggagcgc ctgcagacgc gcagtcgccc tttccagttc
       61 ggcttgcgct ccggctccgg catctccctg cgcaacatcg cgccgggcgc attcgagttc
      121 gatttccgca ccgccgcggg cgctctcgtt cttcagttgc atttcattgt tgcgaatcat
      181 cccttcgatc agcttgtgcc gggcaatcat ccgctcctgg gcgcgctgca gttcagcggc
      241 ttcctcggcg gtgatctgtg gtccgcgccc gctggcgtct gtcactcgca ttactcgcgt
      301 tggatattgc ggactgaaac tgagcatcgt gcggcaaatt tcctacggga ttttcctacg
      361 ggatcaggac ggcgcgccca tgaatatgcc catgatgcag atcatgaagc gcatcgttgg
      421 cctggctgag cttgtattct ttggtatgga ggttgacgag gccgcgatcc gcgagcgcca
      481 tcagttcgac gagttccgga taagtgccga ccagattgcc gacgatggtc ttttccgtcg
      541 tgatcatgtc gatggtcggc agctcgatct tgccgccata acccacgatg taatagtagc
      601 cgccgttggc tgtcatcgac aggcctttgg caatcgcatc gccctcgccg acgaaatcga
      661 tcacggcttc ggcgccccgc ccaccggtca gcgcgagcac ggcttcgact tcgttgccgt
      721 cggccttcac gagatggtgc gcaccgcact ccttggcgag atcgagagac ttgtcggcgc
      781 ggtcgatcac gatgatttcc gccgcacaga gcgctgcgag aacctggatg ccgatatgcc
      841 caagtccgcc ggcgccaatc accgcgacat attcgccggg cagcagatgg cgggatgctt
      901 tcttggcggc gcgataggcc gtcagcccgg cgtcggtgta tggagcgaca tccttcggcg
      961 ccagcgactt tggtaacttg atgagggcgc gctggcccgt gagcaggtat tgcgcgtaac
     1021 cgccgtttgc gttgatgccg ggaaacgaac tatccagtgc atgcatgtcg ttgccgcgcc
     1081 ggcacgcgag acaatgtccg ctggtcacca gcggatggca aatgacactg tcgccaactt
     1141 tgacgccctc gacgccgggc ccgatcgcct cgacccagcc cgcattttcg tgacccatga
     1201 tgtacggaag tttgacgtcg accttgctgc gccaaatgcc ttcgacgata tgcagatctg
     1261 tgcgacagac gcccgcaccg ccaacgcgaa cgatcacgtc catcgggcgc gaaatcttcg
     1321 ggtctgtcac atcctcgtat ttgacaaact cttttgccgt cagtttttca tcaaaggcat
     1381 ggagcaccgc ggctttcatt tatttatctc tccccgttgg cggctttttt tattttttgg
     1441 ttgcagcgcg agcgccatct gatcaactaa atgagcaagg cctgtgccaa gccgataaca
     1501 agttgatctt tttgacgttc tctgcatttt ggcgagttcg tgccgctccg catcggacca
     1561 aacttgacgg cgtttctgtt ccgagacgga acacttcgaa gacgaccaca acgagtagag
     1621 aggaatttgg attttcaggt atttgatcta tgattggcaa aagcgcggac gctcgaagaa
     1681 gacgccatcc gagctatgac caagaggaaa tcgccgaaca tcagttcagg gaggaccgat
     1741 gatgctgcct gccggccggc gcgtcgagaa tgcatggacg acgcttgagc agcgcggagc
     1801 gccgccagcc gaacttctgt cggccgatat ctacgatagc tggatgcgct gcatttcgtt
     1861 cggcctcgac acgctgcgcc cgccttcgcc ccaatttgtc agccctgcaa tcctgcgcca
     1921 ggaacaacag cgttgttcgc tggtccgtgg cctcgcgctc gctgagatgc acacgctgca
     1981 tcagcagatc gccggttcca atttcatgat tgcgtttgcg aacgcagatg gattgctgct
     2041 cgacataata tccgacccga gcttcagcga cgcttcgaac gcggcgagca tccggccggg
     2101 tactgtttgg acggaaagca tttgcggcac caacggcctt ggcaccgcgg cacatctcaa
     2161 gcgcgcgatt gtcgtgcacg ggcgcgagca ctttttttcg cgctacaaca atctcacctg
     2221 cgtcgcagcg ccgatcttcg cgcccgatgg cgagctggcc
//
LOCUS       DASWGM010000079         7689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_6894, whole
            genome shotgun sequence.
ACCESSION   DASWGM010000079 DASWGM010000000
VERSION     DASWGM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6894"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VGA15_02505"
     CDS             complement(<1..153)
                     /locus_tag="VGA15_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX9586602.1"
                     /translation="MTRKQRRLILIGSSLGVLAFAVALVLSSLRESIVFFNSPTDIAE
                     KKSAAGT"
     gene            complement(150..1322)
                     /gene="ccmI"
                     /locus_tag="VGA15_02510"
     CDS             complement(150..1322)
                     /gene="ccmI"
                     /locus_tag="VGA15_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX9586603.1"
                     /translation="MILWPILTLMTLGAVIAVWWPLARRQGSVRSGSDIAVYRDQLDE
                     IDRDQAAGLIGNVEAEAARVEVSRRLIAAAETAKARSTAPAPGPAGRYRRATLAAAIV
                     LLPVGAGSVYLLLGSPGLVPLSMNAATDGQPLPAGVEKTVAEVETYLESNPKNGRGWE
                     LLAPVYLRLGRFDDAVRARRNALEIFGPDAARLGDLGEAMVMASNGVVTAEAKSLFER
                     ANAADPEDVMAQYYLGLSAKQDGRRDEAEKRWRALVSRAGEGAEWLPLVKNALARIDE
                     KSPPVVVAAPAPASSTAKVAPKGPSASEIAAAGQLAPAERTGMIQGMVARLAQKMAEN
                     GSDVDGWLRLIKAYSVLGERDKALAAAANARSALAGNSDNLRRIGELTKELGLEGS"
     gene            complement(1502..1759)
                     /locus_tag="VGA15_02515"
     CDS             complement(1502..1759)
                     /locus_tag="VGA15_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586604.1"
                     /translation="MSDARSYAASLGVDAQRLNLGSGANPTCPATMPAGSNCQFPDVT
                     TRFQRLDATAIFKFDQDIVMARLGKSQLHVHLITASLSYTW"
     gene            2100..3857
                     /locus_tag="VGA15_02520"
     CDS             2100..3857
                     /locus_tag="VGA15_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9586605.1"
                     /translation="MPDLATVTSFGRVILVRGSLARVGLLAVGQIPASEIRATVGRFV
                     SIRCANSTIVAMITEVSCEDLPTSDTYIASASVDLLGEILGGPDRPKFQRGVTNYPTI
                     GDAVELITNQELRTVYAPSGSDQINVGTLQQDPSVIAYVDVEEMLSKHFAVLGSTGVG
                     KSSGVSLLLNEILKARPSLRVFLLDVHNEYGRCFGDRALVLNPRNLKLPFWLFNFEEI
                     VDVLFAGRPGVPEELDILAEVIPTAKGIYTQYQNSDRMGLKRADPKAIGYTVDTPVPY
                     RLVDLISLIDERMGKLENRSSRILYHKLISRIETVRNDPRYAFMFDNANVGGDTMAEV
                     ISHLFRLPANGRPMTIMQLAGFPAEVVDSVVSVLCRMAFDFGLWSDGVSPMLFVCEEA
                     HRYASADRSIGFGPTRKAVSRIAKEGRKYGVYLGLVTQRPAELDATIISQCNTLFAMR
                     LANERDQALLRSAVSDAAANLLSFVPSLGTREVLAFGEGVALPTRLRFKEVPSHQLPR
                     SEATIATVPSVAAGHDMHFVGAVLERWRGATSQRDVPNDPGLNVNDRPAARTVEAPML
                     QPSMGLDPDRFSLLKKPLR"
     gene            3975..4553
                     /locus_tag="VGA15_02525"
     CDS             3975..4553
                     /locus_tag="VGA15_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848430.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX9586606.1"
                     /translation="MTQPIAKRFPTPSIDQLPDDIRTRLLAVQEKSGFVPNVFLTLAY
                     RPDEFRAFFAYHDALMEKDGGLTKAEREMIVVATSSANQCHYCVIAHGAILRIRAKSP
                     QIADQIAVNYRKADITPRQRAMLDFAMKVSAEAHRISEADFIQIAGHGFSDDDIWDIA
                     AISAFFALSNRMANVTAMRPNDEFYMMGRVPK"
     gene            4580..5086
                     /locus_tag="VGA15_02530"
     CDS             4580..5086
                     /locus_tag="VGA15_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX9586607.1"
                     /translation="MALLDGSEIVLRLGVATLAGGLIGLNRDLHGKPIGLKTLGLVGL
                     ATAMVVMLADPFGDSTKLSDAASRIIQGILTGIGFLGAGVIVHAEHHFRVRGLTTAAC
                     TWFAACVGIACGIGQWLIVAVALVIAFALLSVGRRLEHWLHHALGGKADRTPESAASV
                     KPADKPPA"
     gene            complement(5093..5384)
                     /locus_tag="VGA15_02535"
                     /pseudo
     CDS             complement(5093..5384)
                     /locus_tag="VGA15_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
     gene            5498..6394
                     /locus_tag="VGA15_02540"
     CDS             5498..6394
                     /locus_tag="VGA15_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX9586608.1"
                     /translation="MAKSENERTSERSSKARVVQLGGREIVTEGLHLNFWADISHRCM
                     TASWPAFIGGAALVFIAFNAVFAMLYWIGNQPISNVPGGAYIDYLYFSIETLSTAGYG
                     DMHPDTHYGHFIATVELFTGIFSMSLMTGLIFARFSRPNARLLFAENPVISNHEGVPT
                     LMIRLANERHNIISNATARLWAFKTEVSLEGVSFRRFYDLALLRNENPALALSWTLYH
                     VLDEESPLYGLDADGLEASNVSLVVVVSGYDVVAAQTVHARKSYDHPDIRFGQRYVDI
                     LGTAEDGRLKIDYGRFHDTLEG"
     gene            6487..6828
                     /locus_tag="VGA15_02545"
     CDS             6487..6828
                     /locus_tag="VGA15_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586609.1"
                     /translation="MKTQQPITAQDEHRVLKFRPRSSVHPPGRRGESLGKLGKQAQPE
                     DDLARYERDRDEPDDFGHRMLANIAAFAFTVALTAIGIWLAMSIADLRKTQDCVLMGR
                     RDCAHISAPHS"
     gene            7019..7300
                     /locus_tag="VGA15_02550"
     CDS             7019..7300
                     /locus_tag="VGA15_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9586610.1"
                     /translation="MSDTFDQVATIIAETCDIPRDTITPESHAIDDLGIDSLDFLDIA
                     FAIDKAFGIKLPLEKWTQEVNDGKATTEQYFVLKNLSARIDELVAAKGA"
     gene            7310..>7689
                     /locus_tag="VGA15_02555"
     CDS             7310..>7689
                     /locus_tag="VGA15_02555"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEX9586611.1"
                     /translation="MQLEYFQLIDRIADLNVGERTITAEAQVPLQNTIFEGHFPGHPI
                     MPGVLLIEAMAQTSGWLLIALTKFERMPFLAAVKEAKMRSFVSPGELLMVEANVVHEG
                     SGYAITAAKVRVGGELRCNATITFG"
BASE COUNT         1488 a         2439 c         2260 g         1502 t
ORIGIN      
        1 cgtgcctgcc gcggactttt tctcggcgat gtcggtcggc gaattgaaaa acacgatcga
       61 ctcgcgcagc gagctcagca ccagcgccac cgcgaaggcc agcacgccga gacttgagcc
      121 aatcaagatg aggcgccgct gcttccgtgt catgagcctt ccagtcccag ctctttcgtc
      181 aattcgccga tgcggcgcag attgtcgctg ttgccggcga gtgcgctgcg ggcattcgcg
      241 gcggcagcga gcgccttgtc acgctcgccg agcaccgaat aggccttgat caggcgaagc
      301 catccatcga cgtcggagcc gttctcggcc atcttctggg ccaaacgagc gaccatgcct
      361 tggatcattc cagtgcgctc ggccggcgcc aattggcctg cggcggcgat ctcgctcgcg
      421 ctcggcccct ttggtgcgac ctttgccgtg ctcgatgccg gggcgggggc cgcgacaacg
      481 accggcgggc tcttctcgtc gatccgcgcg agcgcattct tgaccagcgg cagccactct
      541 gcgccctccc cggccctgga tacgagcgcg cgccatcgct tctccgcctc gtcgcgacgg
      601 ccgtcctgct tggcgctgag cccgagataa tattgcgcca tcacgtcttc gggatcggca
      661 gcattggcgc gctcgaacag gctctttgcc tccgcggtga ccacgccgtt ggaagccatc
      721 accatcgctt cgccaagatc ccccaggcgc gcggcgtccg gaccgaatat ctccagtgcg
      781 ttccgtcgcg ccctcacggc atcatcgaag cgtccaaggc gaagatagac cggcgccagc
      841 agttcccagc cccgcccgtt cttgggattg ctctccagat aggtctcgac ctcggcgacg
      901 gttttctcga ctccggccgg cagaggttgc ccgtcggttg ccgcgttcat cgatagggga
      961 acgagaccag gcgagccgag caacaaataa acagagccgg cgccgactgg aagcagcacg
     1021 atggcggcgg cgagcgttgc ccggcggtac cggccggccg ggccaggcgc cggcgcggtg
     1081 gatctggctt ttgcggtctc tgccgctgcg atcaagcggc gtgaaacttc gaccctcgcc
     1141 gcctccgctt cgacgttgcc aatcaggccc gcagcctgat cgcggtcaat ctcgtcgagt
     1201 tggtcgcgat agacggcgat gtcgcttcca gagcggacag acccctgccg ccgcgcgagc
     1261 ggccaccaca cagcgatgac cgccccaagc gtcatcagcg tcaaaatggg ccacagaatc
     1321 atctggtcac ttcattgggg ggtgccgcgg cagcctcgct tgagccaact ggcattcaac
     1381 taccggccgg acagaagttc gtcttgatac atatcagacg tcggcaggac agcctaccaa
     1441 aacacgcctg cagtggatct ttcaagaacg agatcgcgtc ccgtcgattt gccgatgcct
     1501 gctaccaggt ataggagagc gacgccgtta tcagatggac atgcaattgg gattttccga
     1561 ggcgagccat gacgatatct tgatcaaact tgaatatcgc ggtcgcatcc aaacgctgga
     1621 accgggtggt cacgtcgggg aattggcagt tgctcccagc cggcatcgta gccgggcatg
     1681 tcgggttggc accgctgccc aaattgagcc gctgagcatc gacgccgagc gacgccgcat
     1741 agctgcgggc gtccgacatt cggtcgcggt ctaaccaccg tcgcacgtct gatatctgca
     1801 gaccgacatg tatgaggcga ggccagccgg gtgtcttccg gaaaaggcgg cgcatcgaac
     1861 agccctcctc ggccgttggt aaaccaggcg ttaaacccga tgcgccgccg gcgccgatgg
     1921 acggacgtca aaaacaccga gtttccagca gaatccgcca acaaagaccc cgccgaggcg
     1981 gcccggcaaa tcgaaatact gaccaagatg tcgcggattt gacgaacccg gggttccctg
     2041 cgcttaaccg tttctccaac ccgaagctgc atagtcatgc actgatgtga attcatgaca
     2101 tgccggattt agccactgtg acatccttcg gacgcgtaat tttggtgcgt ggatccctcg
     2161 cccgggtcgg actgctggcg gtgggccaga tacccgcctc ggaaataagg gcgaccgtcg
     2221 gacggttcgt cagcatccgc tgcgcaaatt cgaccatcgt cgcgatgatc acggaggtat
     2281 cctgcgaaga cctgccgacg tccgacactt atattgccag cgcctcggtc gatctgctcg
     2341 gcgaaatcct cggcgggccg gaccgcccca aattccagcg cggtgtcacc aactatccca
     2401 cgatcggcga tgccgtcgag ctgatcacca atcaggagct ccgcacggtc tatgccccga
     2461 gcggatcgga tcagatcaat gtcggcacct tgcagcagga tccctccgtc atcgcctatg
     2521 tcgatgtcga ggagatgctc tcgaaacact tcgcggtgct cggctctacc ggcgtcggca
     2581 aatcgagcgg cgtatcgctg ctgctgaatg aaattctcaa ggcaaggccg agtttgcggg
     2641 ttttcctgct cgacgttcac aacgaatatg gccgctgttt cggcgaccgc gcgctggtgc
     2701 tcaatccgcg caatctgaag ctgccgttct ggttgttcaa tttcgaagag atcgtcgacg
     2761 tgctgttcgc gggccggccc ggcgtgcccg aggaactcga cattctcgcc gaagtcattc
     2821 cgacggcgaa gggaatctat acgcagtatc agaacagcga tcggatggga ttaaagcgcg
     2881 ccgatcccaa agcgatcggc tacaccgtcg atacgccggt tccctaccgc ctggtcgacc
     2941 tgatttcgct gatcgacgag cgcatgggca agctcgagaa ccgctcatcg cgcatcctct
     3001 atcacaagct gatttcgcgc atcgaaaccg tgcgcaacga tccgcgctat gcgttcatgt
     3061 tcgataacgc caatgtcggc ggcgacacca tggcggaagt gatcagccat ctgttccggc
     3121 tgccggccaa cggcaggccg atgaccatca tgcagctagc cggcttcccc gccgaagtcg
     3181 tcgactccgt ggtgtccgtg ctgtgccgca tggcgttcga tttcggtttg tggagcgacg
     3241 gcgtttcgcc gatgctgttc gtctgcgaag aggcccatcg ctacgcctcg gccgaccgca
     3301 gcatcggctt cggaccgacg cgcaaggcgg tctcgcgtat cgccaaggaa ggccgcaaat
     3361 acggcgtata tctcggcctc gtcacccagc gtcccgccga actcgatgcc acgatcattt
     3421 cccagtgcaa taccctgttt gcgatgcggc ttgccaacga acgcgaccag gcactgctgc
     3481 gttcggcggt ctccgatgcc gcggcaaacc tgctgtcgtt cgttccctcg ctcggtaccc
     3541 gcgaagtcct ggccttcggc gaaggcgtgg cgcttccgac ccggctgcgc ttcaaggaag
     3601 tgccgtcgca tcaattgccg cgcagcgaag ccacgattgc aacggtcccg tcggttgccg
     3661 ccggccacga catgcatttc gtcggcgccg tgctcgagcg ctggcgcgga gctacgtcgc
     3721 aacgcgacgt tcccaacgat cccggcctca acgtcaacga ccggccagcc gcgcgcacgg
     3781 tggaggcgcc gatgctgcag ccgtcgatgg ggctcgatcc cgaccgcttc tcgctcttga
     3841 aaaagccgct gcgctgaagt tttggagccg ctgttttcct acgccgttcg cgcctgcaag
     3901 ctttgcgcga gatgcggcca tcgactatga tcccggacaa ccgcatcacg cggcgtgaaa
     3961 gccgaaatcc ctcgatgacc cagcccattg caaagcgctt tccgaccccc tcgatcgacc
     4021 aactgccgga cgatatccgc acccgcctgc tcgcggtgca ggagaaatcc ggcttcgttc
     4081 cgaacgtgtt cctgacgctg gcctatcgcc ccgacgaatt tcgggcgttc ttcgcctatc
     4141 acgatgccct gatggagaag gatggcggcc tgaccaaagc cgaacgcgag atgatcgtgg
     4201 tggcgacctc cagcgccaac caatgccact attgcgtcat tgcgcacggc gcgatcctgc
     4261 gcattcgggc caagagtccg cagatcgccg accagatcgc ggtcaactac cgcaaggcgg
     4321 atatcacgcc gcgccagcgc gccatgctcg acttcgcgat gaaagtgagc gccgaggcgc
     4381 acaggatttc agaagcggat tttatccaga tcgccggcca cggtttcagc gacgacgaca
     4441 tttgggatat tgccgcgatt tcggcgttct tcgcactgtc gaaccggatg gcgaacgtca
     4501 ccgccatgcg gccgaacgac gaattctata tgatggggcg cgtgccgaaa taggccgttc
     4561 gaaccttaaa aaggaggaca tcgctttgct tgacgggtcc gagattgtgc tgcgtcttgg
     4621 cgtcgccacg ctggcgggcg gcctgatcgg tcttaaccgc gatctgcacg gcaaaccgat
     4681 tggattgaag acgcttggcc tggtgggtct tgccaccgcc atggtcgtga tgctggcgga
     4741 tccatttggc gacagcacga aactctccga tgccgccagc cgaatcatcc agggaatcct
     4801 caccggcatc ggttttctcg gcgccggcgt catcgtccat gccgagcatc attttcgggt
     4861 tcgcgggctc accaccgccg cctgtacctg gttcgcggcc tgcgtcggca tcgcctgcgg
     4921 catcgggcaa tggctgatcg tcgcggtcgc ccttgtgatc gcgttcgcgc ttctaagcgt
     4981 cggccgccgg ctcgagcatt ggctgcatca cgctctcggc ggcaaggccg atcggacgcc
     5041 agaatccgcc gcgtcagtca agccggcgga taagccgccg gcttagccat cctcaatcgt
     5101 cggacgccgg tccgcaccag cccggcagat agaccgcgtc cttgctcttc gccaaagcca
     5161 gcgctttttc gagggacttt tcgaaattgg cttccacgtt cttgcgcgac aagcttcggc
     5221 gcagaaaatc cgcccacagg aattcgctga agggcgtggt gtccttggca aaaccgccag
     5281 cgcttgcgtt tttccttaac ttctctcatt ccgacggtca tctgggtcgg atgcagcgag
     5341 agaatcggaa tcggatgaag gatcggctcg cgcgtattcg ccatgacttg aaacccctga
     5401 cctgacggca aaaaaccctc tgaaatgggt tcgattatgc catgatggca cagtcgacta
     5461 aatgcctttg ccaagtgcct caagagattg gagaaagatg gccaaaagcg agaacgagcg
     5521 gacatccgag cggtcgtcca aggcacgggt cgtccagctt ggcggccgcg aaatcgttac
     5581 cgagggcctt cacctcaatt tttgggccga cattagccat cgctgcatga cggcctcatg
     5641 gccggctttc attggcggcg ccgcactggt gttcatcgcc ttcaacgcgg ttttcgcaat
     5701 gctctattgg atcggaaatc agccgatctc gaacgtgccc ggcggcgcct atatcgacta
     5761 tctctatttc agcatcgaga cgctttcgac cgccggttac ggcgacatgc atccggacac
     5821 ccactacgga cattttatcg cgacggtgga actgttcacg ggcatctttt cgatgtcgct
     5881 gatgaccggg ctgatctttg cccggttctc acgaccaaac gcccggttgc tgttcgcaga
     5941 aaacccggtc atttcaaatc atgagggcgt gccgaccctg atgatcaggc tcgccaacga
     6001 gcgtcacaac atcatcagca atgccaccgc caggttgtgg gcgttcaaga ccgaagtgag
     6061 cctggaaggg gtgtcgtttc gccggttcta cgacctcgca ctgctgcgaa acgagaaccc
     6121 ggcgctggcc ttgagctgga cgctctacca tgttctcgat gaggagagcc cgctctacgg
     6181 acttgacgcc gatggccttg aggcatcgaa cgtttcgctg gtggtggtgg tgtcgggata
     6241 tgacgtcgtt gccgcgcaaa cggtccacgc acgaaagtcc tacgatcacc cggatattcg
     6301 tttcgggcaa cgctacgtgg atatcctcgg caccgcggaa gatggccgac tcaaaattga
     6361 ttacggcagg ttccatgaca ccctggaggg atgattatcg catccgtgcc atgtcaacaa
     6421 tttctgcctg gcgcatcgcg agcgtgctaa attggataag gtgaggaagt cccggagctg
     6481 gcagcagtga aaacccagca acccattacg gcgcaagacg agcaccgcgt gctcaaattc
     6541 cggccgcgca gctcggttca cccgccgggt cggcggggag aatccttggg caagttgggc
     6601 aagcaggctc aaccagagga cgacctggcg cgctacgagc gggaccggga tgagccggac
     6661 gactttggtc atcgcatgct ggcgaacatt gcggcctttg cctttaccgt cgcgctgacc
     6721 gcgatcggaa tatggctcgc catgagcatt gcggacctgc gcaagaccca ggactgcgtg
     6781 ctgatggggc ggcgcgattg cgcgcacatt tctgcaccgc acagctagcc ttcccagtcg
     6841 gcacacgctt ttttgcctca atccgatccg accaccacag cgcgcctcca ttgaactcgg
     6901 cgcggcgctc cgatatacgg gcacctcgac tccggatggc ggaccgggca ttcgggggcc
     6961 ccgcacccgc ctcccgtgcc atctcggaat ttaccttcaa tatatcaaag gtttagtgat
     7021 gtccgataca ttcgatcagg tcgccacgat tatcgctgaa acctgcgaca taccgcgcga
     7081 cacgatcacg ccggagagcc acgcgatcga cgatttgggc attgacagcc tcgattttct
     7141 tgacatcgcg ttcgcgatcg acaaggcgtt cgggatcaag ctgccgctgg aaaaatggac
     7201 gcaggaagtc aacgacggca aggcgacaac cgaacagtac tttgtcctga aaaacctcag
     7261 cgcccgcatc gacgaattgg tcgcggccaa gggcgcgtaa tcgcccatca tgcagcttga
     7321 atatttccaa ctgattgacc gcatcgccga cctcaatgtc ggagagcgca ccatcacggc
     7381 cgaggcgcag gtaccgctgc aaaataccat cttcgagggt cactttccgg gccatccgat
     7441 catgccgggt gtattgctga tcgaggcgat ggcgcagaca tccggctggc tcttgatcgc
     7501 cctgacgaag ttcgagcgca tgccgttcct ggccgcggtc aaggaagcca agatgcgaag
     7561 tttcgtcagc cccggcgaac tgctcatggt cgaggcgaac gtcgttcacg aagggtcggg
     7621 ttatgcgatt acggccgcca aggtccgcgt tggcggcgaa ttaagatgca acgccacgat
     7681 cacgttcgg
//
LOCUS       DASWGM010000080         4431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2363602,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000080 DASWGM010000000
VERSION     DASWGM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363602"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /gene="mtnK"
                     /locus_tag="VGA15_02560"
     CDS             <1..828
                     /gene="mtnK"
                     /locus_tag="VGA15_02560"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046522 - S-methyl-5-thioribose kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HEX9586612.1"
                     /translation="LFRTSDLAMPAGTKKAAQALFCQNTALCKISEDLIFTDPYRVAA
                     LNRWTSPELDGLAAAFRADAPLKIAVQELKWAFLTRGGALLHGDLHTGSVMVTETDTR
                     VIDPEFAFFGPIGFDVGALLANLMLSYLAQNGHPGEPDAIAAYRHWLLDTIEAVWTGF
                     ETKFLALWHTSATGDAFVAELFADPAGAAALEAYRRATMARIFADSVGFAGAKMVRRI
                     LGLAHVEDLESIEDPKIRSACETKALHLARSLMLDRAAIGDVRRLRARIEAADAVTS"
     gene            825..1910
                     /gene="mtnA"
                     /locus_tag="VGA15_02565"
     CDS             825..1910
                     /gene="mtnA"
                     /locus_tag="VGA15_02565"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX9586613.1"
                     /translation="MNIDGKHYRTIWPHADGAIVEVIDQTRLPHEFATLRLANLSEAA
                     EAIRSMVVRGAPLIGVTAAYGLALAMRSDASDDGLTQAYQALLKTRPTAVNLRWALDA
                     MRARIAPLSPSARPEAAWTRAAEIADEDVAISQRIGVAGLPLIETAYAKTGRPVNILT
                     HCNAGWLATVDWGTATAPIYMAHDAGIPVHIWVDETRPRNQGAALTAYELGHHGVPHT
                     IVADNAGGHLMQHDMVDLCIVGTDRTTARGDVCNKIGTYLKALAAHANGVPFYVALPQ
                     TTIDWTIEDGVRDIPIEQRDPREVSHMTGRLDKGSLATVQVTAPGSPVSNFAFDVTPA
                     EYVTALITDRGVCKASADGLRDLFGAR"
     gene            1960..2751
                     /locus_tag="VGA15_02570"
     CDS             1960..2751
                     /locus_tag="VGA15_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9586614.1"
                     /translation="MDFIELDGVALRYELTGKGDHTLVLVHEMGGSLESWDDVAPRFA
                     KSRRVLRYDTRGAGMSQKVRGELSLDTMADDIIALLETLGIAGKVALAGIAVGGAIAL
                     HFAARYPERASAVAVGSPATGIAPDRRAAALERVAKIEASGMAYAVEDSMRNGYAPEL
                     RDDIGRFERFRARWLGNDPASYATIWRMLAGADMQGELTRLRCPVLVIGGSLDRVRPP
                     PLAEAVARAIPGARYVEVRTGHYMAVQTPDLISDCIDEFLRTVGA"
     gene            2793..3680
                     /locus_tag="VGA15_02575"
     CDS             2793..3680
                     /locus_tag="VGA15_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9586615.1"
                     /translation="MSPQPTQQIPGVYHRRIGDIVVTAISDGYLDGNLDVMRNVDLEK
                     AHQVLRDAFRPARRTSVNAFLIHSKGRMAIVDTGSGNYLQPTAGFVQRNLAAAGIDPK
                     STDTVLLTHMHPDHSAGLTDMSNGQLLFPNAELVMHENEPAHWFDDGAMTKVDERSRK
                     LYFLAGREQVAPYKNRTRLFRQGEVFPGVTAVPSLGHTPGHTAYLIASGNEQLMIWGD
                     TVHVPEVQTAFPEAGMAFDTDLAAAAASRKRMFDRVSADGILVAGMHMHFPAFSRLAR
                     RGDAYAIFPEAWVHTMDAI"
     gene            4190..>4431
                     /locus_tag="VGA15_02580"
     CDS             4190..>4431
                     /locus_tag="VGA15_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9586616.1"
                     /translation="MRLPTALGLVSALALAVAAASAVADDRHGRDSDDARTATPIKHV
                     VVIFQENVSFDHYFGTYPKAMNLAGETPFAASQHTP"
BASE COUNT          857 a         1452 c         1308 g          814 t
ORIGIN      
        1 ctgttccgca cgtccgatct cgccatgccc gccggcacca agaaggcggc gcaagccctg
       61 ttttgccaga acaccgcgtt gtgtaaaatc agcgaagacc tcatcttcac ggacccctat
      121 cgagtagccg ccctgaaccg ctggacctcg cccgagcttg acggactcgc ggcggcgttc
      181 cgcgccgatg cgcctctgaa gatcgcggtg caggaactca aatgggcctt cctcacgcgc
      241 ggcggagcgc tgctgcacgg cgatctccat accggttcgg tgatggtgac ggagaccgac
      301 acccgtgtca tcgacccaga attcgcgttc ttcggcccca tcggtttcga tgtcggcgcg
      361 cttctcgcca atctcatgct cagctatctc gcgcagaacg gccaccccgg cgagccggac
      421 gcgattgctg cctaccgcca ctggctgctc gacaccatcg aggcggtctg gaccgggttc
      481 gaaaccaagt ttctcgcgct ctggcacaca tcggcgaccg gcgatgcatt tgtcgccgaa
      541 cttttcgccg atccggccgg cgcggcggcg ctcgaggcat atcggcgggc taccatggcg
      601 cggatcttcg cggacagcgt cggctttgcc ggcgccaaga tggtgcgccg cattctcggc
      661 ctcgcgcatg tcgaggatct cgaatcgatc gaagatccaa agatccgctc cgcctgtgaa
      721 acgaaggcct tgcatctggc gcgcagcctg atgctcgatc gtgccgcgat cggcgatgtc
      781 aggcggctgc gtgcgcgcat cgaggccgcc gacgcggtga cgtcatgaat atcgacggaa
      841 agcactaccg caccatctgg ccgcacgccg acggcgcaat cgtcgaggtg atcgaccaga
      901 cccggctgcc gcacgaattc gcaacactgc gtctggccaa tctctccgaa gcagctgaag
      961 ccatccgctc catggtcgtt cgcggcgcgc cgctgatcgg cgtcaccgcc gcttatggcc
     1021 tcgcactcgc gatgcgttcc gatgcttccg acgacggact gacgcaagcc tatcaggcgc
     1081 tgctcaagac ccgtcccacc gcggtcaacc tgcgctgggc actcgatgcg atgcgagcac
     1141 ggattgcgcc gctctctccc tccgcgcggc ccgaagccgc ctggacgcgc gcggcggaaa
     1201 tcgccgatga ggatgtcgcc atctcgcagc gcattggcgt tgccggcctg ccactgattg
     1261 aaacggcata cgccaagacc ggacgcccgg tgaatatcct cacccattgc aatgccggct
     1321 ggctcgccac ggtagactgg ggaaccgcca ccgcgccgat ctacatggcg catgatgcgg
     1381 gcatcccggt gcacatctgg gtggatgaaa cgcgcccgcg caatcagggc gcagcgctga
     1441 cggcctacga actcggccac cacggcgtgc cgcacacgat cgtggccgac aatgccggcg
     1501 gtcacctgat gcagcatgac atggtcgatc tctgcatcgt cggtaccgac cgcacgacgg
     1561 cgcgcggcga cgtgtgcaac aagatcggca cttacctgaa agcgctggcg gcccatgcga
     1621 acggtgttcc gttctatgtc gcgctaccgc aaaccaccat cgactggacc atcgaggacg
     1681 gcgtgcgcga tatcccgatc gaacagcgtg atccgcgcga ggtgagccac atgaccggac
     1741 gcctcgataa aggcagcctc gcgaccgtcc aagtcacggc gccgggttcg ccggtgtcca
     1801 acttcgcgtt cgacgtcacg ccggctgaat atgtgacggc gctgatcacc gatcggggcg
     1861 tttgcaaggc atcggcagac ggactgcgcg atctgttcgg agcgcggtaa cggtcttgag
     1921 gcaaagcgga tcaagtcgaa gagaaaccag gaacgcagaa tggatttcat cgaattagac
     1981 ggcgtcgcgc tacgctatga gctaaccggc aagggtgacc acacgctggt gctcgttcac
     2041 gaaatgggcg gctcgctcga aagctgggac gacgtcgcgc cacggttcgc gaaatctcgc
     2101 cgcgtattac gctacgatac ccgcggggca ggaatgtcgc agaaggtgcg cggcgagctc
     2161 agcctcgata cgatggccga cgatatcatt gcgctgctcg aaactctcgg aatcgccggc
     2221 aaggtagcgc ttgcgggcat tgccgtcggt ggtgcgatcg cgcttcattt cgccgcccga
     2281 taccctgagc gcgcaagcgc cgtcgcagtc ggcagcccgg cgaccggcat cgcgccggat
     2341 cgccgcgccg cggccctcga acgcgtcgca aagatcgagg cctccggcat ggcatacgct
     2401 gtcgaagatt cgatgcgcaa cggctatgcc ccggagcttc gcgacgacat cggacgcttt
     2461 gagcgattcc gggcgcgatg gctcggcaac gatcccgcga gctatgcgac gatctggcga
     2521 atgctggcag gcgcggacat gcaaggcgaa ctgaccaggc tgcgctgtcc cgtgctcgtg
     2581 atcggcggca gtcttgaccg cgtgcgcccg ccgccgctcg cggaagccgt ggccagagcc
     2641 atccccggcg cccgctacgt cgaagtccgc accgggcact atatggctgt gcaaacgcct
     2701 gatctcatct ccgactgcat cgacgagttt ctgagaaccg tcggcgcctg atcgaacctg
     2761 accatccttc atacgcatca aggtcttctc atatgagtcc gcaacccaca cagcagattc
     2821 ccggcgtcta tcatcgcagg atcggagaca tcgtcgtcac agccatcagc gacggctatc
     2881 tggacggcaa cctcgacgtg atgcgcaatg tcgatctcga aaaggcgcat caggttctgc
     2941 gggacgcatt tcggccggca cggcgaacca gcgtcaacgc cttcctgatc cattcaaagg
     3001 gccgcatggc gatcgtcgac accggatcgg gcaattatct ccagcctacc gccggcttcg
     3061 ttcaacgcaa cctcgcggcg gccggtatcg atccgaagtc caccgatacg gtgctgctga
     3121 cccacatgca ccccgatcat tccgccggcc tgaccgacat gtcgaacggc caactcctgt
     3181 ttccgaacgc ggagctggtc atgcacgaga acgaaccggc gcactggttc gacgacggcg
     3241 cgatgacgaa agtcgacgag cgatcaagaa aactctattt tctggccggc cgcgagcagg
     3301 tcgcgcccta caagaatcgc acgcgattat tcagacaggg cgaggtgttt cccggcgtga
     3361 ccgccgtacc gagcctcggg catacgccgg gccacaccgc gtatctcatt gcgtcgggca
     3421 acgagcagtt gatgatctgg ggtgacacgg tccacgtgcc ggaagtgcaa accgcatttc
     3481 ccgaagccgg catggccttc gataccgatc tcgccgcggc cgcggcttcg cgcaagcgca
     3541 tgttcgatcg ggtctctgct gacggtatct tggtcgccgg catgcacatg catttcccgg
     3601 cattctcacg gctggcgcga cgaggcgacg cctacgcgat cttccccgag gcctgggttc
     3661 acacgatgga tgcgatctag cgagttgatt cgacggtgtc gctcccaggc aatacccacg
     3721 ccatttggcg gcgagcaatc taacccgtcg cgtgaggtct cggccatata acgtcggtaa
     3781 tgctccgcgg cttcctcaag tggtgcagcg ttggcaattc gtgcccccgc tcgctgcgcc
     3841 gcgttttccc acatactcca atttttctgt gagaacgaac gtttctctgt gagaacgaac
     3901 gcccgcggat agccggcctg cgatgcccgt ctgctctctc gcgcttcggc atgaaacatc
     3961 aattggggga ttcggctccg cctcgtccgc cgttcatcca ttcgaatgag atgtcagaag
     4021 ccaacagaat aagatgtcac aaaaatgaga tgtcacaaaa atgagatgtc acaaattttt
     4081 cgtcagtttg tgaccgagct ctgaaaagac tcggcggcgc ttaaccttgg tcgccacaac
     4141 gcactcagaa cattttcaat ttcagctcac gaacgaggag gttatcccaa tgcgtttgcc
     4201 aacagcctta ggacttgtat ccgctctggc ccttgcggtc gcagctgcaa gcgccgtcgc
     4261 cgacgatcgg cacggccgtg attccgacga cgctcgcacc gcgacgccga tcaagcatgt
     4321 tgtcgtgatc ttccaggaaa acgtttcgtt tgatcattat ttcggtacct atccgaaagc
     4381 catgaatctt gccggcgaga cgccgttcgc ggcgtctcaa cacacgccga a
//
LOCUS       DASWGM010000081         5315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2363745,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000081 DASWGM010000000
VERSION     DASWGM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363745"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VGA15_02585"
     CDS             complement(<1..419)
                     /locus_tag="VGA15_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HEX9586617.1"
                     /translation="MNTFEHLVVGIVGPAKSRGAIVLVAVSLLGASSASAQDYRGTSD
                     QQSACMSDVFRLCSGEIPDVGRIVACLKRERPRLSDGCRAVFGGASTRVAANHRARHH
                     RQLGPEQYQSGHYSQYGYQNYQGYQYQGYQSYRGYQG"
     gene            complement(611..1576)
                     /locus_tag="VGA15_02590"
     CDS             complement(611..1576)
                     /locus_tag="VGA15_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9586618.1"
                     /translation="MTADEVSGGWRSSERYPDPAVEGLDPSFAKYRIFSAAVERLATG
                     MRWGEGPVWFGDGRYLLWSDIPNNRILKWEEETGQVSAFRKPSNYANGNTRDRQGRLV
                     TCEHGGRRVTRTEYDGSIKVILDRYDGKPLNSPNDVVVKSDGSIWFTDPPFGILGNYE
                     GSQATPELPQNVYRVDAASGRASVVAGDLRGPNGLCFSPDETKLYLVESRATPNRLIR
                     VYDVVDGGKRLANDRVFVDAGPGTPDGFRCDVDGNLWCGWGMGSPELDGVVVFSPEGQ
                     LIGRIRLPERCANLCFGGAKRNRLFMAASRSLYSLYVATQGAPGG"
     gene            complement(1658..2125)
                     /locus_tag="VGA15_02595"
     CDS             complement(1658..2125)
                     /locus_tag="VGA15_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9586619.1"
                     /translation="MAASAGAADKKAIERGKYLVSFGGCFDCHTPGYFFGKPDMARYL
                     GGSDVGFEMPGLGVFVGPNLTPDPETGLGEWSEAQIVRALQTGKRPDGRELAPIMPWR
                     AFANLTKSDAHAIAAYLKSLPAVSHRVPGPFGPDEKASVFRMKVVPPEAAAAK"
     gene            2614..2958
                     /locus_tag="VGA15_02600"
     CDS             2614..2958
                     /locus_tag="VGA15_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586620.1"
                     /translation="MANGDLHEQIARLEVHIEELAEAIDRCRKIILVSKLAMAIGGIL
                     ILATLVRAIGFDPVAVTVAIASMIGGTVVFGSNTSTRKQMEAAKQSAEAARAELISQI
                     DLRVIGDDSYQG"
     gene            complement(3070..3507)
                     /locus_tag="VGA15_02605"
     CDS             complement(3070..3507)
                     /locus_tag="VGA15_02605"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9586621.1"
                     /translation="MTIQAIQPQAGRNPRTVEEARAFVAHVEKLFMPWNIDALVEGFT
                     QDCEVRFGNVQLRGGSALRAFFEARSARQRNYRLRKRLRAFAGDMLTNIWEGEWEDAQ
                     TGAGMQGFGVELWVMRAGKIAVWEAAFNVCRADADAGVEAMLR"
     gene            complement(3600..3884)
                     /locus_tag="VGA15_02610"
     CDS             complement(3600..3884)
                     /locus_tag="VGA15_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9586622.1"
                     /translation="MKKKGRIGSPFDGFLKQEGIYEEVTARAIKRVIARQLDALMQEQ
                     RLCKSELAKRMRTSRAQLDRVLDPENESVTLGTLTRAARAVGRQLRMELV"
     gene            complement(3881..4243)
                     /locus_tag="VGA15_02615"
     CDS             complement(3881..4243)
                     /locus_tag="VGA15_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9586623.1"
                     /translation="MVRECGERKGEAVTCCFLRSAVGREPVREWLKALDDDDRKVIGE
                     DIKDVEFSWPVGMPLCRALGKGLWEVRSELALGRIARVLFCIHQGQMVLLHAFVKKTQ
                     KMPDADRDLGRKRMKDLT"
     gene            4422..5030
                     /locus_tag="VGA15_02620"
     CDS             4422..5030
                     /locus_tag="VGA15_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586624.1"
                     /translation="MDHTKDIMDVVKFFQYTFIIVFALALGEAFKQFVAEKANQPPDK
                     VVDWGRLPALLSFVVLIFPFYQGMSRYFFATYADVNTLPKPYSLYLVFDSVAFLAESA
                     LFFVMSRALSEDRWRTYYGTVLVLLVVDSSWGAVSWYCHGGPTTVWITLNLSFAVVLL
                     LILLLTPRQRSWVGAMAGLIAVSIRTMLDYALTWQLYFPPPP"
BASE COUNT          941 a         1756 c         1598 g         1020 t
ORIGIN      
        1 tagccctggt agccccggta gctctgatag ccttggtact ggtagccctg ataattctga
       61 tagccgtatt gggaataatg gcccgactgg tactgctccg ggccgagttg gcgatgatgc
      121 ctcgcccggt ggttcgccgc gaccctggtg gaggcgcccc cgaataccgc ccggcagccg
      181 tcgctcaggc gcggccgctc ccgtttcagg cacgccacga tgcggcccac gtccgggatt
      241 tctccggagc acagccggaa cacgtcggac atgcacgccg attgctggtc cgaggttccc
      301 cggtaatcct gggcggatgc gctgctcgcg ccgagaagcg aaacggcgac cagtacgatc
      361 gcaccacgag atttcgccgg cccgacgatc ccgaccacca gatgttcgaa tgtattcatc
      421 acggtccccc ttacacgcct gccccctaac taggccgagt gttctttcat atcagattcg
      481 tggccgttcc gggtgcgcgg gcgacctttc cccccggatt ctcatggaga gtgttcggat
      541 tgcatcaccc gccggtaacc cgatgacggc cggctcgttc ggcgcaagga tggcccggcg
      601 caacggagct tcagccgcct ggcgcgccct gggtggcgac gtagagcgaa tagagcgacc
      661 ggctcgcggc cataaacagg cggttgcgct tggccccgcc gaagcaaaga ttggcgcagc
      721 gttccggcag gcggatgcgg ccgatcagct ggccctcggg cgagaacacc acgacgccgt
      781 cgagctccgg ggatcccatg ccccagccgc accacagatt gccgtcgacg tcgcagcgga
      841 acccgtccgg cgtccccgga ccggcgtcga cgaacacccg gtcgttggcg agcctctttc
      901 cgccgtcgac cacgtcgtag acgcggatca ggcggttggg cgtggcgcgc gattcgacca
      961 gatagagctt cgtctcgtcg ggcgagaaac atagcccgtt gggcccgcgc aggtcgccgg
     1021 cgacgacgct cgcgcgtccg ctcgcggcat cgacgcggta gacgttctgc ggcaattccg
     1081 gcgtcgcctg gctgccctcg taattgccga gaatgccgaa cggcgggtcg gtgaaccaga
     1141 tcgagccgtc ggatttcacc acgacgtcgt tgggcgaatt gagcggcttg ccgtcgtatc
     1201 ggtcgaggat gactttgatg ctgccgtcgt attccgtgcg ggtgacgcgc cggccgccgt
     1261 gctcgcaggt gacgagccgg ccctgccggt cgcgggtatt gccgttggcg tagttcgaag
     1321 gcttgcggaa cgcgctgacc tgcccggtct cctcctccca tttcaggatg cggttgttgg
     1381 gaatatcgct ccacaacaga tagcggccgt cgccgaacca caccggccct tcgccccagc
     1441 gcatcccggt ggcgagacgc tcgaccgccg cgctgaagat ccggtacttg gcaaagctcg
     1501 gatcgagccc ttcaaccgcc gggtcgggat agcgctcgct cgaccgccag ccccccgaga
     1561 cttcatctgc cgtcatcgcg agagccgcac ggccgatgcg ccgctgccga ttctcttcgg
     1621 gtgcatggat tccctcatca cgcgaggcgc agcgccgcta ttttgccgcc gcggcttccg
     1681 gaggcacgac cttcatgcgg aacaccgagg ccttctcgtc gggaccgaac gggccgggga
     1741 cccgatggct gacggcgggc aggctcttga ggtacgcggc gatcgcatgc gcgtcggatt
     1801 tggtgagatt ggcgaacgcg cgccacggca tgatcggtgc gagctcgcgg ccgtccggcc
     1861 gcttgccggt ctgcaacgcc ctcacgatct gcgcctcgga ccattcgccg agcccggtct
     1921 ccgggtcggg ggtcaagttc ggcccgacga acacgccgag tcccggcatc tcgaagccga
     1981 cgtcggagcc gccgagataa cgcgccatgt ccggcttgcc gaaaaaatat cccggcgtat
     2041 ggcaatcgaa acagccgccg aagctgacga gatatttgcc gcgctcgatg gccttcttgt
     2101 cggccgcgcc ggccgaagcc gccatcgtca ccagcgccac caccgccgca aatgcaaggc
     2161 gagatccgtt ccgcaccatg tcccctccgc tcgcaatccc ctggaagcgc cgctccctag
     2221 cacaccggcc ggcacgatgg cagccttgac tttccggccg acccggcttt tgccgcaagc
     2281 aagtagcggg attgcccgcg cctcccaccc cttcgcgcgt gcgaaggcga tccggttcct
     2341 gcgcctggcc cgccgtccgc cggcgcgtgc gccgacatcc cgctgtccga gctggcgctg
     2401 gaatcgttct ttcccgcgat acggccacgg cagaatcgtt gtgcgccttg gcggacgcgc
     2461 cggcccagcg cagcatcgtc tcgatgccgc ttgcgcgaga ctgcctgcga cgccaacggc
     2521 ggagttctct cgcgctttaa aatgaatgta cgcccggata tattcgttcg gatgcaagtg
     2581 cttctcgcga aatggatcta gagtagatcc catatggcca atggtgacct gcacgaacaa
     2641 atcgcgcgtc tcgaagttca cattgaggaa cttgctgaag ccatagatag gtgccgaaaa
     2701 atcattctgg tgtcgaagct ggccatggcg atcggaggaa tattgatctt ggccacttta
     2761 gttcgagcga tcggtttcga tcctgttgcc gtgaccgtcg cgattgcatc catgatcggc
     2821 ggcaccgtag ttttcgggtc gaacaccagt acgcgaaagc agatggaggc agcaaagcaa
     2881 tccgcggaag ctgccagagc cgagttgatc agccaaatcg atcttcgcgt gataggggac
     2941 gacagctatc agggttgacc cgcctaacac cagcccgacc aaaacggttc ggcacgatgc
     3001 cgacacgcag ggatatgcca agcctgccgc cacggccgag gcattgcgcg cctcggcgga
     3061 tgcagcgggc tagcgcagca tcgcctcgac gccggcgtcc gcatcggcgc ggcagacgtt
     3121 gaacgccgcc tcccacaccg cgatcttgcc cgcgcgcatg acccagagct cgaccccgaa
     3181 gccctgcatg ccggcgcccg tctgcgcatc ctcccactcg ccttcccaga tattggtcag
     3241 catgtcgccc gcaaacgccc gcagccgctt gcgcaagcga tagttccttt gccgggcgct
     3301 acgggcctcg aagaacgccc gcagcgccga gccgccgcgc aattgcacat tgccgaagcg
     3361 cacctcgcag tcctgcgtga agccctcgac cagcgcgtcg atgttccacg gcatgaacag
     3421 tttttcgacg tgcgccacga aggcccgagc ttcctcgacc gtgcgcggat tgcggccggc
     3481 ttgcggttgg atcgcttgga tcgtcatgtt tgcctcctgg tcgattgctt gtggtgttcc
     3541 atcaacggtg gcgttccatc aatgggtaca agcaggaaca ccgcgagcgc tgccggcgtt
     3601 cataccagct ccatgcgcag ctgccggccg acggcacgcg ccgcgcgagt gagcgtgccg
     3661 agcgtcaccg attcgttctc gggatccagc acgcggtcga gctgcgcccg gctcgtccgc
     3721 atccgcttgg ccaattccga cttgcacagg cgctgctcct gcatcagcgc gtcgagttga
     3781 cgggcgatga cgcgcttgat cgcgcgcgcc gtcacctctt cgtagatgcc ctcctgtttc
     3841 agaaatccgt cgaacggcga cccgatccga cccttctttt tcatgtgaga tccttcattc
     3901 gttttcgtcc caagtcgcga tccgcatccg gcatcttctg cgtctttttg acgaaggcat
     3961 gaagaagcac catttggcct tggtggatgc agaacaagac gcgagcgatc cgtcctagcg
     4021 ccaattcgct gcgcacttcc cacagcccct tgccgagcgc gcggcagagc ggcatgccca
     4081 ccggccagga aaactccacg tccttgatgt cctcgccgat gactttgcga tcgtcatcat
     4141 caagagcctt cagccattcg cgaacaggct cgcggccgac ggcagagcgt aaaaagcagc
     4201 aggtaaccgc ttcacctttt ctctccccgc attcccgtac caaaaatgat actagcgggc
     4261 aacagaaaaa acccgccgag caggttcaag tgcccggcag gcccgctcct gaccggccgg
     4321 gacgcgccgc tcggaccgcc ggccgcggcc ggcacgcttg acaagagtgt tttttccgga
     4381 ttcccagtac cgcgtggaag cacgatctcg agggggccag gatggatcac acgaaggata
     4441 tcatggatgt cgtaaaattc tttcagtaca cattcatcat cgtgtttgcc ctggcactgg
     4501 gtgaggcatt caaacagttt gtcgcggaga aagccaatca gccgccggac aaagtcgttg
     4561 actggggcag actgcccgcg ctgctcagct ttgttgttct catatttcca ttctaccaag
     4621 gcatgagcag gtattttttc gccacgtacg cggacgtaaa cacactgccg aaaccgtatt
     4681 cactttattt ggtgttcgac agcgtcgcat tcctggcgga gtcggcactg ttttttgtca
     4741 tgtcccgcgc cttgtccgag gacagatggc gtacctatta tgggacggtc ttggttcttc
     4801 tcgtggtgga ttcgagttgg ggcgcagtgt cttggtactg tcacggcggg cctacgaccg
     4861 tgtggatcac gttgaatctc agcttcgccg tggtgctcct gctgatcttg ttgctgaccc
     4921 cccggcaaag atcctgggtc ggcgccatgg ccgggctcat cgcggtgtcg atcagaacga
     4981 tgctcgatta cgcgttgacg tggcagctgt attttccgcc gcctccctga cggcatttgg
     5041 catccgcgcg ccccagggct gatgtcttca ctgtcatcgg cggccgacgt ccgatactgg
     5101 gcacctctgg gaatcaaccg gacgtgctag tccgcaaagc gctgaaaagc ggaccttgtc
     5161 gccctcgctc aaccatctcg aacacaaata gcgtccggtt gccggtttac accctccggt
     5221 tcccagcggt ttccaggctg cgcaggggga aaagcaaaac cccgccagca tgtgattggc
     5281 gagcaaaaca gggtgcactc cccaatctgg gagac
//
LOCUS       DASWGM010000082         7491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1668943,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000082 DASWGM010000000
VERSION     DASWGM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668943"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VGA15_02625"
     CDS             complement(<1..408)
                     /locus_tag="VGA15_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9586625.1"
                     /translation="MTGKRVVFTGGTGKAGRHVLPHLKSKGYDLLNVDLKPFGHPGIS
                     TLIADLTHSGQAFNALTTHFGFDRFEAGRPPAAPDAVVHFAAIPRVLIEPDNETFRVN
                     TVSTYNVIEAAAKLGVRKIVIASSETTYGVCFAE"
     gene            complement(507..1505)
                     /locus_tag="VGA15_02630"
     CDS             complement(507..1505)
                     /locus_tag="VGA15_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586626.1"
                     /translation="MDIQFDDGIKSGRFPILSWLTSRQAFWVLLAVCVACVFLSFATN
                     SFATSNNIFNVTRNFTFVAVVALGMTIVIITGGIDLSVGSVLCLCSMILAVVMHAGYS
                     IEVGIAAALGTALLIGAINGVLIAYLGMPPFVVTLGMLSIARSLAMVASNNTVVFQFG
                     PDQDKLLFLGGGALLFGIANPVIYMLVLALLTGFLLRFTRFGRYVFAIGGNEHAAIVT
                     GVPVRRTKIIVYMISALSAGIAGVIETGWLGAVTTNLGNGMELQVIAATVIGGANLAG
                     GIGTAFGALIGSALIEVIRNSLGLLGISAFWQGTFVGSFIIVAVAFDRIRNFRSPT"
     gene            complement(1543..2286)
                     /locus_tag="VGA15_02635"
     CDS             complement(1543..2286)
                     /locus_tag="VGA15_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9586627.1"
                     /translation="MALLELENVSKHFGAIHALNDVSLSLEPGEVVGLMGDNGAGKST
                     LAKIVAGNFRPTHGAMRMEGRDLVMHRPIEARQHGIEIVHQDLALCDNLSAAANVFLG
                     REVRKGFGPFKVLDYATMYRRAGQIFAELRSETRPRDLVRQMSGGQRQAVAIARTRIA
                     DAKLILMDEPTAAISVRQVAEVLNLIRLLRDRGIAIVLISHRMPDVFSIADRVVVLRR
                     GRKVADKDVAASSPEEVTGLITGAIEHAL"
     gene            2799..3791
                     /locus_tag="VGA15_02640"
     CDS             2799..3791
                     /locus_tag="VGA15_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9586628.1"
                     /translation="MNKTLLALTVAALTLSAGSAMAQKKQLVIVVKGLDNPFFEAIHQ
                     GCEKWNKENATSPYNCFYTGPASTSDEAGEAQIVQDMLGKSDTVAMAISPSNAPLIAN
                     AIKAAKPKVPIMTLDADLKKEDASLRKTYLGTDNYLLGFRLGEYLKKAKPNGGMFCLI
                     EGNAAADNILRRASGTRDSLSGKKDLDRLKGEGGWTEVSGCPVFTNDDGPKGVQAMSD
                     ILTANPKLDAIVIEGGWPLFGAPQPYRQLTDQYKAQIASKSLVIVAADTIGDEVAIAK
                     EGRVEALVGQRPFEMGYKAPSVMISLIEGKKVDDPLFTGLDECTKATADTCVQK"
     gene            complement(3907..4008)
                     /locus_tag="VGA15_02645"
                     /pseudo
     CDS             complement(3907..4008)
                     /locus_tag="VGA15_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
     gene            4339..5784
                     /gene="xylB"
                     /locus_tag="VGA15_02650"
     CDS             4339..5784
                     /gene="xylB"
                     /locus_tag="VGA15_02650"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEX9586629.1"
                     /translation="MYLGIDLGTSAVKTILVDHEQRVVASRSRSLTVNSPHPGYSEQD
                     PGQWIDATFATLDALKADHPRELAAVDGIGLSGQMHGATLLDANFKPLRPCILWNDGR
                     SAAECRMLEKRWPALRATTGNKAMPGFTAPKLVWVAKHEPEIFAATKLVLLPKAYLRL
                     ILTGEAIEDVSDASGSLWLDVARRDWSDEGLSATGLSRSQMPRLVEGCAPAAPLRSEM
                     ALRWGMTKRPMIAGGAGDNPAGAVGIGAIRPGAAFVSLGTSGALLVPTNAIAANPQRA
                     VHTFCHAIPAMWIQAGAILSAASSLAWIARLLGTSEADLLEPLGLRPKAPSLVNFLPY
                     LSGERTPHDDPEARGMLDGLSHGTDRAAIVQAVLEGVAFAIADCRDALADTGIAISEA
                     DAIGGGSRSRFWLSVLANVLNIPIHRFAEGETGAAFGAARLGRLAVTGETVEAVCTPP
                     RRIETFEPEHALADAYAERLPKWRNLYRPRR"
     gene            6137..>7491
                     /locus_tag="VGA15_02655"
     CDS             6137..>7491
                     /locus_tag="VGA15_02655"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX9586630.1"
                     /translation="MSDPILEMRRVSKSFFSIKALRNVDLTVYASEIHALMGENGAGK
                     STLMKILSGAYRPDPGGEIRIEGRLVRIDGPLGGRAAGISIIYQELSLAPNLNVAENI
                     YLGREVSHSGLLARGAMRASVGPMLERLGADFAPSSLVAHLSMGQRQLVEIARALHAR
                     SKILIMDEPTTALSAGESERLFALIRQLRAEGLAIIYISHRMDEVYALGDRVTVLRDG
                     TLVGSLDKPQIRADTIVRLMVGREVSSFYKKDHDPQARLGPPALAAVDIADGRRVKGC
                     SLTVHAGEVVGLAGLIGAGRTELAHLMIGASPKTSGHVELEGRAVTIRTPGEALDAGI
                     AYLTEDRKALGLFLDMSCLDNINLAVLGRDAKLGFILDRDKARDRANNAFTALGIRAP
                     DIGVTAGGLSGGNQQKVLLSRLLAITPKVLILDEPTRGVDVGAKSEIYSIIDNLAKSG
                     TA"
BASE COUNT         1472 a         2277 c         2342 g         1400 t
ORIGIN      
        1 ttcggcaaaa cagacgccgt aggtggtctc gctggaggcg atcacgatct tgcggacgcc
       61 gagtttcgcc gcggcttcga tgacattata ggtgctgacc gtgttgacgc gaaacgtctc
      121 gttatccggc tcgatcagta cccgcggtat cgcggcaaaa tgcacaacgg cgtccggcgc
      181 cgccggcggc ctgccggctt cgaaacggtc gaagccgaaa tgcgtggtca gggcgttgaa
      241 ggcctggccg ctgtgcgtca gatcggcgat cagggtgctg atgccgggat gaccgaacgg
      301 cttgaggtcg acattgagaa ggtcgtagcc cttgctcttc agatgcggca ggacatgcct
      361 gccggccttg ccggttcccc cggtgaatac cacgcgcttg cctgtcattt gctttctcgt
      421 ccgtcgttga tcgaatggtg gagacgtgct gtccggttac ttccgctggc gggaacgcca
      481 tcccgaaatc catgatccgg ccgcgctcat gtcggcgatc ggaaattccg gatccggtcg
      541 aaggccacgg caacgatgat gaacgagccg acaaaggtgc cctgccagaa cgcgctgatg
      601 cccaatagcc cgagactgtt gcggatgacc tcgatcagcg ccgaaccgat cagggcgccg
      661 aacgccgtgc cgatgccgcc ggcaagattg gcgccgccga tcacggtagc ggcgatcacc
      721 tgaagctcca tgccgttgcc gagattggtg gtgaccgcgc cgagccagcc ggtttcgatt
      781 accccggcaa taccggccga gagcgcggaa atcatataga cgatgatctt ggtgcggcgc
      841 accggcacgc cggtcacgat cgcggcatgt tcgttaccgc cgatggcgaa gacgtagcgg
      901 ccgaatctgg tgaagcgcag caggaagccg gtcagaagcg cgagcacgag catgtagatg
      961 accggattgg cgattccgaa cagcagcgcc ccgccgccga ggaacaacag cttgtcctga
     1021 tccggcccga actgaaacac cacggtgttg ttggatgcga ccatggccag gctgcgcgcg
     1081 atcgacaaca tcccaagcgt gaccacgaac ggcggcatgc cgagataggc gatcagcacg
     1141 ccgttgatgg cgccaatcag gagcgcggtt cccagcgcgg cggcgatgcc gacctcgata
     1201 ctgtagccgg catgcatgac gaccgcgaga atcatgctgc acaggcagag caccgaaccg
     1261 accgacaggt cgatgccgcc ggtgatgatc acgatcgtca tgcccagcgc gacgaccgcg
     1321 acaaaggtga aattgcgggt tacgttgaag atgttgttgg acgtcgcaaa ggagttggtc
     1381 gcgaacgaca gaaagacgca ggcgacgcac accgccagca gaacccagaa ggcctggcgc
     1441 gacgtcagcc aggagaggat cgggaatctg ccggacttga tcccgtcgtc gaactgaatg
     1501 tccattgcca acaccttcga ttcttcggaa gccgtcatgc ggtcacaacg cgtgttcgat
     1561 cgcgccggtg atcagcccgg tcacctcctc cggtgaactc gcggccacgt ccttgtcggc
     1621 gaccttgcgg ccgcggcgca gcaccacgac gcgatcggcg atgctgaaca catccggcat
     1681 gcgatggctg atgagcacga tggcaatgcc gcgatcgcgc agcagccgga tcagattcag
     1741 cacctcggcg acctgccgca ccgaaatcgc cgccgtcggc tcgtccatca gaatgagctt
     1801 ggcatcggcg atccgggtcc gggcgatcgc caccgcctgt cgctgcccgc ccgacatctg
     1861 ccggaccaga tcgcgtggcc gcgtttccga tcgcagctcg gcaaagatct ggccggcgcg
     1921 gcgatacatg gtggcgtagt cgagaacctt aaacgggcca aaacccttgc ggacttcgcg
     1981 gccgagaaag acgttggcgg cggcgctgag attgtcgcag agcgccagat cctggtgcac
     2041 gatctcgatg ccgtgctggc gggcttcgat cggccgatgc atgaccagat cccggccttc
     2101 cattcgcatc gcgccatgcg tcgggcggaa atttccagcg acgatcttgg ccaaggtgga
     2161 cttgccggcg ccgttgtcgc ccatcaggcc gacgacctcg cccggctcca gcgacagcga
     2221 aacatcattg agggcatgaa tggcgccgaa atgcttcgaa acattttcaa gttcgagaag
     2281 agccattcca gaattcacct tcggcggaat tgccgcccga tgctcgtctt cagtttccgt
     2341 aaatcaacca cggctcacgg cgtcgaagga gccggcattg aattacagtg agtgtagcca
     2401 gtcaatgttc tccaccgggc ttgcggcggc cccgttcgcg aacaaccaaa tgatgtgcag
     2461 cgcaccaaat agagccttgc gaaattcgtg ccgaacaaat gcgttgactc atcacaggcc
     2521 cgaattatcc tcacggctcg ctgatatcga tatcttgagc ggaattcggt agtcatagca
     2581 aacccctcac atcaagcgtt tcttgatttc ccattttccc cggcgcgtga ggatccaatg
     2641 gcctcgtgag gatgcgagtg caatcggtat ggcgttcgac ttcgacccaa tctccgattc
     2701 agtgagccga cacatgaatt agacaattgg acaactcgaa tcagatcaat tctggaaaac
     2761 gggcgaataa acgcccgctg cagatgtggg aggcaccaat gaacaaaacg ctattggcat
     2821 tgaccgttgc ggcgctgact ttgtcggcgg gatccgcgat ggctcagaag aagcagctcg
     2881 tcatcgtcgt gaagggtctc gacaatccgt tcttcgaagc cattcaccag ggttgcgaga
     2941 agtggaacaa ggaaaacgcg acttcgccgt ataactgctt ctacaccggg ccggcttcga
     3001 cgtcggacga ggccggcgag gcccagatcg tgcaggacat gcttggcaag tccgacaccg
     3061 tggccatggc gatttcgccg tcgaacgcgc cgttgatcgc caatgcgatc aaggcggcca
     3121 agccgaaggt gccgatcatg acgctcgacg ccgacctcaa gaaggaggat gcgtcgcttc
     3181 gcaagaccta tctcggcacc gacaattacc tgctgggctt tcggctcggc gagtacctga
     3241 agaaggccaa gccgaatggc gggatgttct gcctgatcga gggcaacgcc gcggccgaca
     3301 atattctgcg tcgcgcatcc ggcacacgcg attcactgtc cggaaagaag gatctggacc
     3361 ggctgaaggg cgaaggcggc tggaccgaag tctcgggctg cccggtcttc accaatgacg
     3421 acggacctaa gggggtccag gcgatgtcgg acattctcac cgccaacccg aagctcgatg
     3481 ccatcgtgat cgagggcgga tggccgctgt tcggtgcgcc gcaaccctat cgccagctga
     3541 ccgatcaata caaggcgcag atcgcgagca agtcgctggt gatcgtcgcc gccgatacga
     3601 tcggcgacga ggtggcgata gcgaaggaag gtcgcgtcga agccctggtc ggccagcggc
     3661 cgttcgagat gggctacaag gcgccgtcgg tcatgatcag cctgatcgaa ggcaagaaag
     3721 tcgacgatcc gctgtttacc ggcctcgacg aatgcaccaa ggctaccgcc gacacttgcg
     3781 ttcaaaaata gccgagcatc ccgcgcaggg atcgagcgcg gtcgcgggtt cgcatgaagg
     3841 agcgggaggc gcgtggcgcc tccctttttg ttttcatttg aattttggac ggccggatat
     3901 cccgcgtcat gacagcctga cccgcggctc gggacggccg atggcaccga cgtcgagaat
     3961 gaaagtccgg ccatggtgcg gatcggccgc cctggcttgc gccatcactg cgacgacctt
     4021 gcgcgcgttc gactggtcgt caacaacgag attgcgcatg gattttaggc tcaactagtt
     4081 tcatcgcggc ttgggatgaa aggctgacgg atgccacacc ggcgcgtcct tgagctgcaa
     4141 aattccgtag gtcagcttat ggtgcatggc acacgaaacc aaccgagcga acgcccccgg
     4201 attgtcgtgg aaatggcgcc gccgtgcctg tcgctattga cgagtaacgg ctgcgcctgt
     4261 tcgatacggg agcggatatc gtctctgtgt actatgtccg ttgtgtcgat ccacttcgcg
     4321 cgtcaggcga gaggcatcat gtatctcggt atcgatctcg gcacgtcagc ggtcaaaacc
     4381 attctcgttg atcatgagca gcgggtggtg gcaagccgaa gccgatcgct gacggttaat
     4441 tctccacatc ccggctattc cgaacaagac ccagggcagt ggattgatgc tacgtttgca
     4501 acgctggacg ccttgaaggc ggatcatcct cgcgaattgg cggcggttga cggcatcggc
     4561 ctgtcgggcc agatgcacgg cgcaactctg ctcgatgcga acttcaaacc gttgcggccc
     4621 tgcattcttt ggaatgacgg acggtccgct gcggaatgcc gtatgctgga aaagcgctgg
     4681 cccgcattgc gtgcgacgac aggcaacaag gccatgcctg gattcaccgc tcccaaactg
     4741 gtgtgggttg cgaaacacga accggagatc ttcgcggcca ccaaactcgt tttgctcccg
     4801 aaagcatatc tacgcctgat tctgaccggt gaagcgatcg aggatgtctc ggacgcatcg
     4861 ggctcgttgt ggctcgatgt cgcgcgccgg gactggtcgg atgaagggct gtccgcaacc
     4921 ggcttgtcgc gcagccagat gccgcgtctc gtcgagggct gtgcgcctgc ggcgccgctg
     4981 cggagcgaga tggcccttcg ctggggaatg accaaacgac cgatgatcgc cggaggcgcc
     5041 ggcgacaatc cggcgggcgc cgtcggcatc ggtgcgatac ggcccggcgc cgcatttgtt
     5101 tcgctcggaa cctccggagc attgctggta ccgaccaatg caattgccgc caacccacag
     5161 cgggcggtgc atacgttctg tcacgccatc cccgcaatgt ggattcaggc cggtgcgatt
     5221 ctctcggcgg catcgagtct cgcctggatc gcgcgattgc ttgggacctc tgaggccgac
     5281 ttgctggagc cgctcggttt gcgcccgaag gcgccgtcgc ttgtaaactt tttgccgtat
     5341 ctgtccggcg aacggacgcc gcacgatgac ccggaggcgc gcggaatgct cgacgggctg
     5401 agccacggca cggatcgtgc cgcaatcgtg caagccgtgc tcgagggcgt tgccttcgcg
     5461 atcgcagatt gtcgggacgc gcttgcagac accgggatcg cgatctcgga ggctgatgcg
     5521 atcggtggcg gttcacgttc gcggttctgg ctgtcggtgc ttgcaaacgt tctgaatatt
     5581 ccgatccatc gatttgccga gggtgaaacg ggagcggcgt ttggtgcggc gagactgggg
     5641 cgcctggctg tcactggcga gacggtcgaa gccgtctgca caccccctcg acgcatcgag
     5701 acattcgaac ctgagcacgc actcgcagat gcttacgccg agcggcttcc caaatggcgc
     5761 aacctgtacc gaccgcggcg ctaatgccgc ccccgacact agtgccaccg atttgaagtt
     5821 cctacatcgg ttgccgcgag ctcgtcccag gaacttcaaa atcgaaaagc ggcattagga
     5881 tcatagattt gccagggccc ccttggttcc gaagttcgtg tcagaagctg ttgcgatgta
     5941 tcacgaactt cggaagcagg gcaccaggtc ggttccggaa ggcgatgtcc cggttcgtgg
     6001 catcggcgag ccgcggtttc ctcgatattg ccctgcgccg ccgatttttg tttaatgagt
     6061 aaactcgcgc tgccctgcaa acgagccgcg ggtgagaaac gcattggcgc cgtcggcgcg
     6121 ccggggagga ggcacgatga gcgacccgat cctcgaaatg cgccgggtct ccaagtcttt
     6181 cttcagcatc aaggcgttgc gcaatgtcga cctgaccgtc tacgcgagcg aaatccatgc
     6241 tttgatgggc gagaacggcg ccggcaaatc gacgctcatg aagatattgt cgggcgcata
     6301 caggcccgat ccgggtggcg agatccgcat cgagggcagg ctggtgcgca tcgatggtcc
     6361 cctcggcggt cgcgcagccg gcatctccat catctatcag gagctctccc tcgcccccaa
     6421 tctgaacgtc gcggagaata tctatctggg ccgcgaagtc tcacattctg ggttgctggc
     6481 gcggggcgcc atgcgagcca gcgtcggccc gatgctcgag cggcttggcg cggatttcgc
     6541 gccgtctagt ctggtggccc atctgtccat gggccagcgg caactcgtcg agattgcccg
     6601 tgcgctgcac gccagatcga agatcctgat tatggacgag ccgaccaccg cgctttcggc
     6661 cggtgagagc gagaggctgt tcgccctgat ccgccagctg cgcgctgagg gtctcgccat
     6721 catctatatc tcgcatcgca tggatgaagt atatgctctc ggcgaccggg ttacggtgct
     6781 gcgcgacggc acgctggtgg gctcgctcga caagccgcag atccgggccg acaccatcgt
     6841 tcggctgatg gtgggacgcg aggtctcgtc cttctacaag aaagaccatg acccgcaggc
     6901 gaggttaggg ccccccgccc tcgcggccgt cgacatcgcc gatgggcggc gcgtcaaggg
     6961 ttgctccttg accgtgcatg caggcgaggt cgtcgggctt gccggcctta tcggcgcggg
     7021 ccgcaccgag cttgcgcatc tcatgatcgg ggcttcgccc aagacctccg gccatgtcga
     7081 actcgaaggc cgtgcggtca ccatacgcac acctggcgag gctctcgatg cgggcattgc
     7141 ctatctcacc gaagacagga aggcgctcgg cttgttcctg gacatgtcct gtctggacaa
     7201 catcaacctc gccgtgctgg gcagggacgc caagctcggc ttcatcctcg acagggacaa
     7261 ggcccgcgac cgtgccaaca acgccttcac ggcgctgggc atccgcgccc cggatatcgg
     7321 cgtcacggcc ggtggactgt ctggcggcaa ccagcagaag gtgctgctgt cgcgcctgct
     7381 cgcaatcacg cccaaggtcc tgattctcga cgagccgacg cgcggcgtcg atgtcggggc
     7441 caagtccgag atctattcga tcatcgacaa tctcgcaaaa tccggcaccg c
//
LOCUS       DASWGM010000083         3747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_837761,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000083 DASWGM010000000
VERSION     DASWGM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837761"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGA15_02660"
     CDS             <1..261
                     /locus_tag="VGA15_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX9586631.1"
                     /translation="GGTFAPGSPWSICITPPPNQVLYSSDAWPGRIYKLSLEGKVLGM
                     LGQSGKQLKQFGWIHAMACPSENVIYAAELLNWRVQKLILKP"
     gene            complement(345..1202)
                     /locus_tag="VGA15_02665"
     CDS             complement(345..1202)
                     /locus_tag="VGA15_02665"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEX9586632.1"
                     /translation="MDQIAYVNGSFVPLSEAKVSVLDRGFLFADGIYEVAAVLDGKLI
                     DNASHLARLERSVGEIALALPETIDRIQEIQKELVARNNLVNGMVYLEVTRGADTGRD
                     FAFPKDVKPTLVMFTSVKDIVNAPSAKTGIAVITVPDLRWERRDIKSVALLAQVLAKQ
                     AAAEAGAGEAWMIEDGKVTEGGSSSAFILTQDDVLVTRQNSSAILPGCTRKAVVALAE
                     ERQLRVEERPFTVEEALKAKEAFITSATVFVQAVVSIDGKTVANGKPGPMTDRLREIY
                     VDFARATAQ"
     gene            complement(1469..2200)
                     /locus_tag="VGA15_02670"
     CDS             complement(1469..2200)
                     /locus_tag="VGA15_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX9586633.1"
                     /translation="MIEISHVDKWYGPGFQALKDCTTSVAKGEVVVVCGPSGSGKSTL
                     IKCVNALEPFQSGEIILDGIKVNDPKTDLPKLRARVGMVFQHFELFPHLKIIENLCLA
                     QEKVLGRSHDEAVAKSAKLLDRVGLKDHARKYPAELSGGQQQRVAIARALAMDPIAML
                     FDEPTSALDPEMISEVLDVMVDLAREGMTMMVVTHEMGFASKVAHRVIFMDQGEIVED
                     ALKTDFFGSPRSDRAQKFLSKILSH"
     gene            complement(2300..2992)
                     /locus_tag="VGA15_02675"
     CDS             complement(2300..2992)
                     /locus_tag="VGA15_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508695.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9586634.1"
                     /translation="MFANFDFDVIRRSLGYLFFDGMTFTLTLTGLGALGGLIFGTLIA
                     LMRLSGLRLLGRIAGLYVDLMRSLPLVLVIFWFYFLVPYLGQWLTGSSRPVRVGAFTS
                     SLVTFIMFEAAYFSEIMRAGIQSISQGQPAAANALGLTYAQTMRYVVLPQAFRNMLPV
                     LLTQTIVLFQDTSLVYVLSIPDFLGAASKVAQRDGRLVEMYLFAAAVYFIISSAASFS
                     VRRLQARISIVR"
     gene            complement(3000..3731)
                     /locus_tag="VGA15_02680"
     CDS             complement(3000..3731)
                     /locus_tag="VGA15_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9586635.1"
                     /translation="MNYHWNWRIFWEPAPNGTGTYLDMLLSGLVLTIETAICAWILAL
                     VFGSIVGVLRTLPSRTASLVGFAWVEFFRNMPLLVQLFIWFFVLPEILPPSWGLWLKQ
                     MPNAPFYTAAIGIGFFMSARVAEQLRAGIGSLPRGQKLAATALGLTTAQTYRYVLLPM
                     AFRIIMPPLTSEFLSTIKNTSVALTIGLIELTAEARAMQEFSFQVFEAFTAATVLYLL
                     INIVVVIAMRFLERGLAIPGYITGK"
BASE COUNT          789 a         1158 c         1134 g          666 t
ORIGIN      
        1 ggcggcacct ttgcgccggg ctcgccatgg tcgatctgca tcacgccgcc gcccaatcag
       61 gtgctctata gctcggacgc gtggcccggc cgtatctaca agcttagcct cgagggcaag
      121 gtgctcggca tgctcggcca gtccggcaag caactaaagc agtttggctg gatccacgcg
      181 atggcgtgtc cctcggagaa cgtgatctac gcagccgagt tgctcaactg gcgggtgcag
      241 aagctgatcc tgaagccgtg atgtgaaggg cgcgcgactt cacctctccc attgggagag
      301 ggagcgcgcg ggataccgcc tttggcggca agcgagactg agcgctactg cgccgtggcc
      361 cgagcaaagt ccacatagat ctcgcgcaag cggtcggtca tcggaccggg cttgccgttg
      421 gcgacggtct tgccgtcgat cgacaccacc gcctgtacga acacggtggc gctggtgatg
      481 aaggcttcct tggccttaag cgcctcctcg accgtaaatg gccgctcctc gacgcgaagc
      541 tgacgttcct cggcgagcgc taccactgcc ttgcgggtgc agcccggcaa gatcgctgat
      601 gaattctgcc gcgtcaccag cacgtcgtcc tgggtcagga tgaaggcgga ggacgagccg
      661 ccctcggtca ccttgccatc ctcgatcatc caggcctcgc cggcgccggc ctctgccgcg
      721 gcctgttttg ccagcacctg cgccagcagc gccacgctct tgatgtcgcg ccgctcccag
      781 cggagatcgg gcacggtgat caccgcgata ccggtcttgg ccgacggcgc gttgacgatg
      841 tccttgaccg acgtgaacat caccagcgtc ggcttgacgt cttttggaaa cgcaaagtcg
      901 cgcccggtgt cggcgccgcg cgtcacttcc agatagacca tgccgttgac gaggttgttg
      961 cgcgcgacca gctccttctg gatctcctga atgcgatcga tcgtttccgg caaggccagc
     1021 gcgatctcgc cgaccgagcg ctccagccgc gccagatgcg aggcgttgtc gatcagcttg
     1081 ccgtcgagca cggccgcgac ttcatagatg ccgtcggcga acaaaaagcc gcggtccagc
     1141 accgagacct tggcctcact tagaggcacg aacgaaccgt tgacgtaagc gatctgatcc
     1201 aagcgatttc tcctgtcggg cagcgaagcg gctacgtggc acagtatatg aagattgtta
     1261 aggcctgatc gacgaaatta aaaacggaat tcgccgcgag gtgtctccac ctctcccatc
     1321 gggagaggtc ggcgcgtagc gccgggtgag gggttacagc ttaacgatag actgtaaccc
     1381 ctcaccccaa ccctctccca caagggggga gggagcgcgc tgccgtctgc gaaacactga
     1441 gtcaaaacca atttcaattc tcaaaatcct aatgcgacaa gatctttgac agaaactttt
     1501 gcgcgcggtc gctgcggggg ctgccgaaaa aatcggtctt cagcgcgtcc tcgacgattt
     1561 caccctggtc catgaaaatc acccggtgcg ccaccttgct ggcaaagccc atttcatggg
     1621 tgacgaccat catggtcatg ccctcgcggg caagatcgac catgacgtcg agcacctcgg
     1681 agatcatttc gggatcgagc gccgaggtcg gctcgtcgaa cagcatggcg atcggatcca
     1741 tcgccagcgc gcgtgcgatg gcgacgcgct gctgctggcc gccggacagt tcggcgggat
     1801 atttgcgcgc gtggtccttc agcccgacgc ggtcgagcag ttttgcgctc ttcgcgaccg
     1861 cctcgtcatg cgaccgtcca agcacctttt cctgcgccag gcaaaggttc tcgatgatct
     1921 tcagatgcgg aaacagctca aaatgctgga acaccatgcc gacgcgggcg cgcagtttcg
     1981 gcaggtcggt ctttgggtcg ttgaccttga tgccgtcgag gatgatttcg ccgctctgga
     2041 acggctccag cgcgttgacg catttgatca gcgtcgattt tcccgatccc gaggggccgc
     2101 acaccaccac cacctcgccc ttggcgacgc tggtggtgca atccttcagc gcctgaaaac
     2161 cgggcccgta ccatttgtcg acgtgactga tctcgatcat gaaggtctcg cgctgtgtga
     2221 ttgcggcaag caaggcgagc tccctctccc tttgggagag gtgatcgaac tcgcacattt
     2281 cgattctatt ttcatggatc taacggacaa tactgatacg cgcctgaagg cgccggacgc
     2341 tgaacgatgc agcgctggaa attatgaaat agaccgcggc cgcgaacaaa tacatttcga
     2401 ccagccggcc gtcgcgctgc gccaccttgc tggctgcgcc caggaagtct ggaatcgata
     2461 gcacatagac cagcgaggtg tcctggaaca gcacgatggt ctgggtcagc agcaccggca
     2521 gcatgttgcg gaacgcctgc ggcagcacga cataacgcat ggtctgcgcg taggtgagac
     2581 ccagcgcatt cgccgcggcc ggctgtccct gggagatcga ctggatgccc gcgcgcatga
     2641 tctcggagaa gtacgccgcc tcaaacatga tgaaggtgac gagcgaggag gtgaacgcgc
     2701 cgacccggac cggccgcgac gatcccgtca gccactgccc gagatagggc accaggaaat
     2761 agaaccagaa aatcaccagc accagcggca gcgaccgcat caggtcgaca tagagaccgg
     2821 cgatgcgccc gagcagcctc aacccggaca gtcgcatcag ggcgatcaac gtaccgaaga
     2881 tcaggcctcc cagtgcgcca agaccagtca gcgtcagcgt aaacgtcatc ccgtcgaaaa
     2941 acagatatcc gagcgagcgg cggatgacgt caaagtcgaa attggcgaac atgcgcggct
     3001 cacttcccgg taatgtagcc aggaatggcg agcccgcgct caaggaagcg catggcaatg
     3061 acgacgacga tattgatcag gagatacagc accgtcgccg ccgtgaaggc ctcgaacacc
     3121 tgaaaggaaa attcctgcat cgcccgcgcc tcggcggtca gttcgatcag gccgatggtg
     3181 agggccaccg acgtattctt gatggtagag agaaattcgg aagtgagcgg cggcatgatg
     3241 atgcgaaacg ccatcggcag caggacataa cggtaggtct gcgcggtcgt tagtccgagc
     3301 gcggtcgcag ccaatttttg tccgcgcggg agcgatccga tgccggcccg caattgttca
     3361 gccacccgcg cggacataaa aaacccgatc ccgatcgccg cggtgtagaa cggcgcattg
     3421 ggcatctgct tcagccagag cccccatgac ggcggcagga tttccggcaa aacgaagaac
     3481 cagataaaca gctgcaccag gagcggcatg ttacggaaga attcgaccca ggcgaagccg
     3541 accaaggatg ccgtcctgga cggcagcgtg cgcaatacgc cgacgatcga tccgaagacg
     3601 agcgccagga tccaggcgca gatcgcggtc tcgatggtca ggaccagccc cgacagcagc
     3661 atgtcgagat aggtgccggt cccgttcggg gccggctccc agaagatccg ccagttccag
     3721 tgatagttca cgaaagtccc ccgcgta
//
LOCUS       DASWGM010000084         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2639890,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000084 DASWGM010000000
VERSION     DASWGM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2639890"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VGA15_02685"
     CDS             complement(<1..588)
                     /locus_tag="VGA15_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX9586636.1"
                     /translation="MSLANNVEFLRRLPKLQGLNGFGDFGRNLRSGTRSPLVEITGFG
                     SNPGGLKMFSFVPDSLQPAPALVVVLHGCGQSATAYDLGAGWSTLAKHYGFALLMPEQ
                     ASLNNAQGCFNWFNPEDTARDSGEACSIRQMIARMVGDHKIDPQRIFVTGLSAGGAMT
                     SVMLATYPEIFAGGAIIAGLPFGVATNVREALSGMF"
     gene            complement(731..2149)
                     /locus_tag="VGA15_02690"
     CDS             complement(731..2149)
                     /locus_tag="VGA15_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9586637.1"
                     /translation="MHQIVSAPVDSSRRWWVLAIVVAAQFMFGVDAFIVNVAIPTIAV
                     ELHASPAQIEAVIAIYLIAYATLVVTGGRLGDIHGTKNVFLVGVFGFSVTSLWCGLAQ
                     SGPELIDARLAQGATAALMVPQVLATLHLLFSDTSRSRAFGIYGIVLGLSGAAGFLLG
                     GVLVTLDLAGLGWRAVFFVNVPFGLVIISAALRIMPSVPRRAGTRLDVPGAVVLFAGL
                     LCLIGPLLFGHDVHWAPWIWLVMAAGVAIVAAFLRLERAVASRGGMPLIDLALLSDAA
                     FMRGLFAAFFFFFANLSFYLVMTMFMQRGLRIPPLQAGSVFLPLALAFVIASRHSGAR
                     ARHRGTRVLIEGCAVQIVGLALLVLTVATDVTPAAQLLASVLAIFGYGQGLVMAPLSS
                     AVLSTVKPASAGSGAGMYGTTQQIANAAGVAAIGAVFFAIEGAGSARLALFAASALFA
                     LSIVVCAAFLSWMRRALPPDTD"
     gene            2320..3711
                     /gene="sthA"
                     /locus_tag="VGA15_02695"
     CDS             2320..3711
                     /gene="sthA"
                     /locus_tag="VGA15_02695"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HEX9586638.1"
                     /translation="MYDYDMVVIGSGPSGRRAAVQFAKLGRSVLVVEKGRRVGGVSVH
                     TGTIPSKTLRETVLNLSGWRERGFYGRSYRVKQDIGAVDLIARLQKTLDHEVEVLEHQ
                     FSRNMVPTACGEGRFVGPNQIEVTAEGGETRMVSAAHVLIACGTRPFRPDYVPFDGRS
                     VFDSDEIIDLPKLPRSLAVIGAGVIGVEYATIFSALDVAVTLIEPRPTFLDFIDKELI
                     EEFMHELRDRSVALRLGSAVNSIERTEGGSIVTKLSDGRNVVSEMLLFAAGRVGATDR
                     LNLEAAGIAVDHRGRIAVDPVTLQTSVPHIYAAGDVIGFPSLASTSMEQGRVAACHAL
                     GMEPMAPPEFFPYGIYSVPEISTAGLTEEEVRARKIPYEVGVARFRETSRGHIMGLNS
                     GMMKMIFSTKTRRLLGVHILGEGATELIHIGQAVLNLKGTIDYFIQNTFNYPTLAEAY
                     KIAGLDAWNRMTR"
     gene            3746..4249
                     /locus_tag="VGA15_02700"
     CDS             3746..4249
                     /locus_tag="VGA15_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586639.1"
                     /translation="MRQFRASKSYTLFHQISALLSRGCKSALLDGLQCEDIIVRSRGN
                     RCEGNPSMRAHMPALAMIALLGFCGQANAQARCPELTQLRSEAAEILKQTRGVPTSDR
                     CAAYVRFSMAWGAIAQYANDNRELCDISIPSLSDFEKYRREAVTVRDNACAGRPLHAF
                     PPDIIRR"
BASE COUNT          950 a         1401 c         1350 g          754 t
ORIGIN      
        1 aaacatgccg ctcaaggctt cccgcacatt ggtcgcaacg ccgaacggca gccctgcgat
       61 gatggcgccg cccgcgaata tttcgggata ggtcgcgagc atcaccgatg tcatggcgcc
      121 gccggcggac agcccggtca caaagatgcg ctgcggatcg attttgtgat cgccgaccat
      181 ccgcgcgatc atctgccgga tcgagcaggc ttcgccgctg tcgcgcgcag tgtcttccgg
      241 attgaaccag ttgaagcatc cttgggcatt gttcaatgac gcctgctccg gcatcagcaa
      301 cgcgaaacca tagtgttttg ccagggtgga ccagccggca cccagatcat aggcagtggc
      361 gctctgaccg caaccgtgca gaacgacgac gagcgcgggc gccggctgca ggctatcggg
      421 cacaaacgag aacatcttga gaccaccggg attcgaaccg aatccggtga tttcaaccag
      481 cgggctgcgg gtacccgacc tcaggtttcg cccgaaatct ccaaagccat tgagaccttg
      541 cagctttggc agacgtcgca ggaattcgac attgttggcg agggacaccg gcagctcctt
      601 gatagagttt ttcataactc caccaaatat agatagttgc tgcactgcaa aataaaaagg
      661 ccgtgtgctg tcagtcccca gatttaaaaa gaccgtgtgc tgtcggtctt aagaattaag
      721 gcaatcgatc ttaatcggta tcaggcggca gcgcgcgccg catccatgac aaaaacgcgg
      781 cacagaccac aatcgacagc gcaaacagcg ccgacgcggc aaatagcgcc aatcgcgccg
      841 agccagcacc ttcaatcgca aaaaacactg cgccgatcgc ggcgacgccg gccgcattgg
      901 cgatttgctg ggtcgtgcca tacattcccg cgcccgagcc cgcgctcgcc ggcttcacgg
      961 tggatagtac ggcgctcgac aacggcgcca tcaccaaccc ctggccgtag ccaaagatcg
     1021 ccaataccga cgcgagcagt tgcgcagcgg gcgtcacgtc ggttgcaacc gtcagcacca
     1081 gtagcgcgag cccgacgatc tgcaccgcac agccctcgat cagcacgcgg gtgccgcgat
     1141 gccgcgcccg cgcaccgctg tgtcgcgagg cgatgacgaa ggcgagcgcc aaagggagga
     1201 acaccgagcc tgcctgcagc ggcggaatcc gcaagccgcg ctgcatgaac atcgtcatca
     1261 ccagatagaa tgacagattg gcgaagaaga aaaagaaggc agcaaacaga ccgcgcatga
     1321 acgctgcgtc ggacaatagc gcaaggtcga tcagcggcat tccaccgcgg ctggcgacgg
     1381 cgcgctcgag ccgcaggaat gcggcaacga tggcgacgcc cgccgccatg accagccaga
     1441 tccatggcgc ccagtgtaca tcgtgaccga acaacagcgg gccgatcagg cacaacagtc
     1501 ccgcgaacaa tacgaccgcg ccgggaacgt ccagccgcgt gccggcgcgg cgcggcaccg
     1561 acggcatgat ccgcaacgcc gctgatatga tgaccagccc gaacggcacg ttgacgaaaa
     1621 acaccgcgcg ccagccgagg cccgcaagat caagcgtcac caacacgccg ccaagcaaga
     1681 agccggccgc acccgaaagg cccagcacga tgccgtaaat gccaaaggcg cggctgcgcg
     1741 aggtgtccga aaacaacaga tgaagggtag cgagcacctg cggcaccatc agcgccgcgg
     1801 tggcgccctg cgccagcctg gcgtcgatca attcggggcc ggattgcgcg agcccgcacc
     1861 acagcgaggt gacgctgaat ccgaatacgc ccaccaaaaa tacatttttg gtgccgtgga
     1921 tatcgccgag ccggccgccg gtgaccacca gcgttgcata ggcgatgagg tagatggcga
     1981 tcaccgcctc gatctgcgcc gggctcgcat gcaattcgac cgcaatggtc gggattgcga
     2041 cgttgacgat gaaggcatcg accccgaaca tgaattgcgc ggcgacgacg atcgcaagta
     2101 cccaccagcg gcgcgacgaa tcgacggggg ctgaaacaat ctggtgcatg gggcagcggc
     2161 cttgtccgaa aatttgtcgc ttcgattgat ctcaggtttc ggtcgtcgcc gcgattacct
     2221 ccgaggtaag gattcgcaag cggtttggcc cgattgctgc atgggaaaaa tgccatcggc
     2281 agctctccag cctgatgcgc gagttcggga ccgcctgcga tgtatgacta tgacatggtg
     2341 gtgatcggat ccggtccttc cggccgccgc gccgccgtgc aatttgccaa gctcggccga
     2401 tcggttctgg tggtggagaa gggccggcgg gtcggcggcg tttcggtcca taccggcact
     2461 atcccgtcaa aaaccctgcg cgaaaccgtg ctcaatctct cgggctggcg cgagcgcggt
     2521 ttctatggcc gctcctaccg ggtgaagcag gatatcggcg cggtcgacct gatcgcgcgc
     2581 ctgcagaaga cgctcgacca tgaagtcgaa gtgctcgaac atcaattcag ccgcaacatg
     2641 gtgccaaccg cctgcggcga agggcgcttt gtcggcccca accagatcga ggtgacggcg
     2701 gaaggcggcg agacccgaat ggtgtccgcc gcccatgtcc tgatcgcatg cggcacgcgg
     2761 ccgttccggc cggattacgt gcccttcgac ggcagatccg tgttcgacag cgacgagatc
     2821 atcgacctgc cgaaactgcc gcgcagcctt gccgtgatcg gtgccggcgt catcggcgtc
     2881 gaatacgcca ccatcttctc agcgctcgac gtcgcggtga cgctgatcga accgcggccg
     2941 acattcctgg atttcatcga caaggagctg atcgaggagt tcatgcatga gttgcgcgac
     3001 cgcagcgtcg cgctgcggct tggctccgcg gtcaattcaa tcgaacgcac cgaaggcgga
     3061 agcatcgtga caaaactgtc cgacggccgc aacgtcgtct ccgaaatgct gctgttcgcc
     3121 gcgggccgcg tcggcgccac cgaccggctc aatctcgagg ccgccgggat cgcggtggac
     3181 catcgcggcc gcatcgccgt cgacccggtg acgctgcaga ccagcgtgcc gcatatctat
     3241 gccgccggcg atgtgatcgg ttttcccagt cttgcctcga cctcgatgga gcagggccgg
     3301 gtcgccgcct gtcatgcgct tgggatggag ccgatggcgc cgcccgaatt cttcccctac
     3361 ggcatctatt cggtgccgga aatctcgacg gccggcctga ccgaggaaga agtgcgggcg
     3421 cgaaagattc cctacgaggt cggcgtggcg cgcttccgcg aaacctcgcg cggccacatc
     3481 atggggctca acagcggcat gatgaaaatg atcttctcga ccaagacccg ccgcctgctt
     3541 ggcgtgcata ttctcggcga gggcgccacc gagctgatcc atatcggcca ggccgtgctc
     3601 aacctcaaag gcaccatcga ttatttcatc cagaatacgt tcaactatcc gacgctggcc
     3661 gaagcctaca agatcgccgg gctcgacgcg tggaaccgga tgacgcggta ggccgtgtaa
     3721 atgcgccgat cagcgaaacg acgcgatgcg ccaattccgg gcatcaaaga gctacacgct
     3781 cttccaccaa atcagtgcct tgctgtcgcg aggctgcaaa tcagccctgc ttgacggctt
     3841 gcaatgtgaa gatattatcg tgcgcagtcg tggcaatcgg tgcgagggaa atccttcgat
     3901 gcgggcgcat atgccagctc ttgcgatgat agcgctactc ggattttgcg gtcaggccaa
     3961 cgcacaggcg cggtgccccg aattgaccca gctgcgcagc gaggctgcag agatcttaaa
     4021 acagacgagg ggcgtcccga catcggaccg ctgcgcagcg tacgttcgtt tttccatggc
     4081 atggggtgca atagcccaat acgccaatga caatcgggag ctgtgtgaca tttccatccc
     4141 ttcgctgagc gatttcgaga aatatcgccg cgaggcagta actgtgcgcg acaatgcgtg
     4201 cgcgggccgc cctcttcacg catttccgcc ggacatcatt cgacgttgag acgctccgac
     4261 ggccgggcca tttcttacaa cgatttctga attggatcag cgcgcaggat gggcggatcg
     4321 ggataggggg gaacctcacg gctcctcccc tcccacacca ccgtacgtac gggtccgtat
     4381 acggcggttc gacagattaa gcacggtcgg tgtttcacgc cgtgccggag agtcagacag
     4441 tggagtccgt cgcaa
//
LOCUS       DASWGM010000085         5739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1810067,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000085 DASWGM010000000
VERSION     DASWGM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810067"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VGA15_02705"
     CDS             <1..1272
                     /locus_tag="VGA15_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX9586640.1"
                     /translation="NAGWSYADVLPYFKRSEDNADFDGEYHGKGGPLAVNKLRTDNPV
                     QQTFLQAAREAQFRIREDFNAEDHEGLGVYQLTQRNGERWSAARAYVHPHMATRANLH
                     VETHAQTTRILFEGKRAVGVEYRQGEATKQMRARREVILASGAFQTPQLLLLSGVGDS
                     AALARHGIATTHHLPGVGQNLQDHPDFIFAYLSDSPYFTGMSFSGIAHQLRAIMQYRR
                     ERRGPMTSNFAECGGFLKTSPDLDIPDIQLHFGMAIVDDHGRKRRWGRGFACHFCLLR
                     PKSRGSVALSSADPLAAPAIDPNFLGHVDDLESMVAGYKTTRRLMETPALRALQKKEM
                     FTADIDTDDDIRALLRARVDTVYHPVGTARMGTDALAVVDPTLKVHGLEGLRIVDASI
                     MPTLIGGNTNAPTIMIGEKAADMIKAELRAN"
     gene            complement(1415..2176)
                     /locus_tag="VGA15_02710"
     CDS             complement(1415..2176)
                     /locus_tag="VGA15_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9586641.1"
                     /translation="MSGLFDVSKEIILVTGASQGLGRQFARVLSAHGAAVALAARQTA
                     KLKSLEEEIRGQGGRAVAVQMDVTNIASIGKAIDSAEAALGPVSVLVNNAGVAIEKPA
                     IDQTEQDWDAVIDANLKGAYFAATEMARRMIARKQEGNIVNIASVLGLGVMKFLSPYT
                     ISKAGIIQATKAMALELAGNRIRVNALAPGYIDTEMNHEFWSSPAGEKLTKRIPQRRV
                     GAEADLDGAIMLLASNASRYMTGSVVTVDGGFLLN"
     gene            2298..2810
                     /locus_tag="VGA15_02715"
     CDS             2298..2810
                     /locus_tag="VGA15_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX9586642.1"
                     /translation="MNSFDAVVYLGLTVAVVTGFNAGLLRSAVTILAYLVAMPIAVWA
                     MSLIASQIDGALGSTWAQNWLLFFGIFLIAGMVLGKLMRMALDETIGSDAGIADRLAG
                     ALLGAVRVGLVATTLVLIFDLVPSDRQPSFLTGSQLRPLLSAAGQKGFKSLPPDVTAY
                     IDRLKRDQRI"
     gene            2962..3747
                     /locus_tag="VGA15_02720"
     CDS             2962..3747
                     /locus_tag="VGA15_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9586643.1"
                     /translation="MDLGIKGRRALVCASSKGLGRACAMALAAEGVHVTLTARGAEAL
                     AKTAAEIRKVSPGVTVTEVAGDITTPAGREAALKACPDPDILINNAGGPPPGDFRNWT
                     RDDWIKAIDANMLTPIELIKATVDGMMARKFGRIVNITSAAVKAPIEVLGLSNGARAG
                     LTGFVAGIARKTVINNVTINGLLPGPFDTDRLRGPVAKIDAEKRGITVDQLLAERAKL
                     NPAGRFGDPEEFGQACAFLCGAKAGFITGQNILLDGGAFPGTL"
     gene            3763..4752
                     /locus_tag="VGA15_02725"
     CDS             3763..4752
                     /locus_tag="VGA15_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEX9586644.1"
                     /translation="MKAVWYERTGAAPDVLTFGDMPTPEAGPGEVRVRLQASGVNPAD
                     VGRRAGSYRAMEFPRVIPNSDGAGIVDQLGAGVTRLSVGQRVWLFNGQRNGRAFGTAA
                     EYIALAEHLVTPLPDNVSFAAGATLGIPGMTAWCGLFGDGPVAGQTVLVTGGAGAVGH
                     YAVQLAKWGGARVIATVSSSFKAEQARLAGADLVIDYKTENVIARAMAFTEQRGVNRV
                     VDVDFGGNIATTLKLMAMNSTIAIYATNGNRSPVVPMRELMERCVTLRALVLFALPQP
                     MLAAAQADISKWLAAAPRIHNIAAQFALPETTQAHLAVEKGDKLGTVVVDCAR"
     gene            complement(4957..5367)
                     /locus_tag="VGA15_02730"
     CDS             complement(4957..5367)
                     /locus_tag="VGA15_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEX9586645.1"
                     /translation="MPELAISAEKVEFLIEKTREFDVKESTSDPDSGSNGADDNMIDV
                     LEDNGDDPVVREITGFINAMTEDERIDLVALMRLGRGDGSIEEWDDLRREAADGDNRH
                     TARYLLGEPLVSDYLAEGLDAFGLDRSDERTTPV"
     gene            5501..>5739
                     /locus_tag="VGA15_02735"
     CDS             5501..>5739
                     /locus_tag="VGA15_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9586646.1"
                     /translation="MHWNVGKVKITKIVELETTGSTRFILPLATNEEIRKLPWLIPHF
                     ANEEGRLKMSIHSLVVETPSRRIVVDTGLGNDKEGR"
BASE COUNT         1062 a         1834 c         1775 g         1068 t
ORIGIN      
        1 aatgccggct ggtcctacgc tgacgtgttg ccttatttca agcgttcgga agacaatgcg
       61 gatttcgatg gcgaatacca tggcaaaggc gggccgctcg ccgtcaacaa actgcgcacc
      121 gacaacccgg tgcagcagac ctttctgcaa gctgcgcgcg aggcgcagtt ccggattcgc
      181 gaggatttca atgccgaaga tcacgagggt ctcggcgtct atcagctgac gcagaggaac
      241 ggcgaacgct ggagcgcggc gcgggcttat gtccaccccc atatggccac ccgcgccaat
      301 ttgcacgtcg agacccatgc gcagaccacc cgcatcctgt tcgagggcaa gcgcgcggtc
      361 ggcgtcgaat acaggcaggg cgaagctaca aaacaaatgc gcgcccgccg cgaagtgatc
      421 ctcgcctccg gcgcgttcca gacgccgcaa ttgctgctgc tgtccggggt cggcgacagc
      481 gccgcgcttg ccagacacgg catcgcaacc acgcatcatt tgcccggcgt cgggcaaaac
      541 ctgcaggacc atccggattt catcttcgcc tatctgtcag acagtcccta tttcaccggc
      601 atgtcgttca gcggcatcgc gcatcagctt cgcgcgatca tgcagtaccg gcgcgaacgg
      661 cgcggaccga tgacttccaa ttttgcggaa tgcggcggat ttctcaagac cagccccgat
      721 ctcgacatcc ccgacattca attgcatttc ggcatggcta tcgtcgacga tcacggccgc
      781 aagcgccgct ggggccgcgg cttcgcctgc catttctgcc tgctgcggcc gaaaagccgc
      841 ggcagcgtcg cgctctccag cgccgatcct cttgccgcgc ccgcgatcga tccgaatttc
      901 cttgggcatg tcgacgatct ggaatccatg gtcgcgggct acaagaccac gcggcggctg
      961 atggagacgc cggcgctgcg ggcgttgcag aaaaaggaaa tgtttacagc tgatatcgat
     1021 accgacgacg acatccgcgc gctcctgcgc gcgcgtgtcg acaccgtgta tcacccggtc
     1081 ggcaccgcca ggatgggcac cgatgctctt gccgtggtcg atccgacact gaaagttcac
     1141 gggctggaag gcctgcgcat cgtcgatgct tctatcatgc cgaccctgat cggcggcaac
     1201 accaacgcgc cgaccatcat gatcggcgag aaagccgcgg atatgatcaa ggcggagttg
     1261 cgggcgaatt gattggccgc atccaccatc gtcatcctga ggtgcgcgcc gtcttcggcg
     1321 agcctcgaag gatgagagtt cagcggatgc ggtcatcctt cgaggcgcgc aagagcgcgc
     1381 acctcaggat gacgatggtg gatgcggcga gctactagtt cagcaaaaat ccgccatcga
     1441 ccgtcaccac gctgccggtc atgtagcgcg aggcgttcga cgccagcagc atgatggcgc
     1501 cgtcgagatc ggcttcggcg ccgacccggc gctgtggaat ccgttttgtc agtttctcgc
     1561 ccgcgggcga cgaccagaac tcgtggttca tctcagtatc gatataaccc ggcgccagcg
     1621 cattgacgcg gatgcggttg ccggccagtt ccagcgccat cgctttggtg gcctggatga
     1681 tcccggcctt ggagatcgta tagggcgaga gaaacttcat cacgcccaag ccgagcacgg
     1741 aagcgatatt gacgatattg ccttcctgct tgcgcgcgat cattcgccgc gccatttcgg
     1801 tggccgcgaa atacgccccc ttgaggttgg cgtcgatgac ggcatcccaa tcctgctcgg
     1861 tctggtctat tgcgggcttt tcaatcgcga cgccggcatt gttgacgagc acgctgaccg
     1921 ggccgagtgc tgcttccgcg ctgtcgatcg ctttcccgat cgaagcaata ttcgtcacgt
     1981 ccatctgaac cgcgacggcg cggccgccct gccctcgaat ttcctcctcc aggcttttga
     2041 gcttggcggt ctggcgcgcc gccagcgcga ccgcggcgcc atgcgcggac aacacccgcg
     2101 cgaattgccg gcccaacccc tgcgaggcgc cggtgacaag aatgatttct ttactgacgt
     2161 cgaatagacc ggacatggct ggtttggttc ccttaggggt cgcgcgcgtt cattgataca
     2221 gacgatttta tggatcgcaa accggtttgg ttgaccaacg ggaaccgcga acgttcggcg
     2281 caggcggaga agtgtccatg aacagtttcg atgcggtcgt ctatctcgga ttgaccgttg
     2341 cggtcgtgac cggtttcaat gccggattgc tgcgcagtgc ggtcacgatc ctggcctatc
     2401 tggtcgcgat gccgatcgcg gtctgggcga tgtcgctgat cgcctcccag atcgacggcg
     2461 cgctcggctc tacatgggcg cagaattggc tgctgttctt cgggatcttt ctgatcgccg
     2521 gcatggtgct gggaaagctg atgcgaatgg cgctcgatga aactatcggg tcggatgccg
     2581 gcatcgcaga ccgcctcgcc ggtgcgctgc tcggcgccgt gcgcgtcggc ctggtcgcga
     2641 cgacgctggt gctgatcttc gatctcgtgc cgtcagatcg ccagccttcg ttcctgaccg
     2701 gctcgcagct gcggccgcta ttgtcggcgg ccgggcagaa gggcttcaag tcgctgccgc
     2761 cggatgtcac ggcgtacatc gatcgcctca agagagacca gcggatataa gacctcgaat
     2821 gtcggccctg tccgggccgc cgcggtccag cctctgatat gcatttcgac cctgcccggt
     2881 ccccttatca aggacgccaa ggttggctac aaggggccgg accggacttc aaaaatcatc
     2941 tgatatgatg ggagtgagac agtggatctt gggatcaaag gccgccgcgc actggtatgc
     3001 gcatccagca agggattggg acgcgcctgc gcgatggcgc tggccgccga gggtgtgcat
     3061 gtcacactga ccgcgcgcgg cgccgaggcg ctggcgaaaa ccgccgccga gatacgcaag
     3121 gtcagccccg gcgtcacggt gacggaagtc gccggcgaca tcacgacgcc tgcgggccgc
     3181 gaggccgcgc taaaagcctg tcccgatccg gacatcctga tcaacaacgc cggcggcccg
     3241 ccgcccggcg atttccgcaa ctggacccgc gacgactgga tcaaggcgat cgatgccaac
     3301 atgctgacgc cgatcgaact gatcaaggcg acggtggacg gcatgatggc ccgaaaattc
     3361 ggccgcatcg tcaacatcac ctccgccgcg gtgaaggcgc cgatcgaggt gctcggatta
     3421 tccaacggcg cccgcgccgg cctcacagga ttcgtcgcag gcattgcgcg caagaccgtg
     3481 atcaacaacg tcaccatcaa cggcctgttg cccggcccct tcgacaccga ccgcctgcgc
     3541 ggcccggtcg cgaagataga cgcggagaag cgcggtatca ccgtggacca gttgctggcc
     3601 gagcgcgcca aactcaatcc ggccggccgc ttcggcgacc ccgaggaatt cggccaggcc
     3661 tgcgcgtttc tctgcggcgc caaggcgggt tttatcacgg gccagaacat cctgctcgat
     3721 ggcggcgcgt tcccgggcac gctctgaatg aaagtgctct gaatgaaggc cgtctggtac
     3781 gagcgaacgg gcgcggcgcc tgatgtgctg acctttggcg atatgccaac gccagaggcc
     3841 ggtccgggcg aagtccgggt ccggctgcaa gcctccggcg tcaatccggc cgatgtcggc
     3901 cgccgcgccg gcagctatcg cgcgatggag tttccccgcg tgattcccaa cagcgatggc
     3961 gccggcattg tcgaccagct cggtgccggc gtcacccggc tcagcgtggg ccagcgggtc
     4021 tggctcttca acggccagcg caacggccgc gcgttcggca cggcggctga atatatcgcg
     4081 ctcgccgagc acctggtgac gccgttaccc gacaacgtct cgttcgcggc cggtgcgacg
     4141 ctcggcattc ccggcatgac cgcgtggtgc ggcctgttcg gcgatgggcc ggtcgcgggc
     4201 cagacggttc tggtcaccgg cggcgcaggc gcggtcggcc actacgccgt gcagctggca
     4261 aaatggggcg gcgcgcgcgt gatcgcgacc gtcagctcat cgttcaaggc cgagcaggcc
     4321 cgacttgccg gcgcagatct ggtcatcgac tacaagaccg agaacgtcat cgccagggcg
     4381 atggcgttca ccgagcagcg tggcgtcaac cgggtcgtcg atgtcgactt cggcggcaac
     4441 atcgcaacga ccttgaaatt gatggcgatg aattcaacca tcgcgatcta cgccaccaac
     4501 ggcaaccgca gcccagtggt tccgatgcgc gagctgatgg agagatgcgt tacgttgcgc
     4561 gcgctggtgc tgttcgctct gccacagcca atgcttgcgg cagcgcaggc cgatatttca
     4621 aaatggcttg ccgccgcccc gcgcatccat aacatcgccg cgcagttcgc gctgccggag
     4681 accacgcagg ctcatctcgc ggtggaaaaa ggcgacaagc tcggcaccgt ggtcgtcgat
     4741 tgcgcgcggt agaatctgat tcttgaattt ggctcgtcgg ttccgcaacc tcggcttacc
     4801 tctccccctg tgggagaggt cggatcgcat cgacagatgc gatccgggtg aggggttagg
     4861 atctatcgat aggccgtaac ccctcacccc aaccctctcc cacaagggga gagggagcgc
     4921 gccgtcgtcg tcgaaacgtc gtaggataaa ctcttatcac accggcgtgg tccgctcgtc
     4981 gctccggtcc aggccgaagg catccagtcc ttcggcgagg taatcgctga ccaggggttc
     5041 gccgagcaga tagcgcgcgg tgtgccggtt atctccgtcg gcggcctcgc gccgcagatc
     5101 gtcccactct tcgatgctgc cgtcgccgcg cccgagccgc atcagcgcca ccagatcgat
     5161 ccgctcgtct tcggtcatgg cattgatgaa gccggtgatc tcgcgcacta ccggatcgtc
     5221 gccattatcc tccagcacat cgatcatatt gtcgtcggcg ccgttcgacc cggaatccgg
     5281 atccgacgtg ctttccttga cgtcgaattc acgggttttc tcgatcaaaa actccacttt
     5341 ttcggccgaa atggctaatt ctggcatcgg tgcgctcctt agcttctcgt gccccaccaa
     5401 cgcgcaggcc agcgagatga tccattgcgc ggagtcacgg aaaccagcat ctttgcgcca
     5461 agaaaccgaa gatgcaacgc gagccaaggg gaagcgccag atgcactgga atgtgggtaa
     5521 ggtcaaaatc acgaagatcg tcgaattgga gacgacaggc agcacccgat ttatcctgcc
     5581 gctggcgacc aacgaagaaa tccggaaatt gccctggctc attccccatt tcgccaacga
     5641 ggaaggtcgt ctcaaaatgt cgatccattc gctggtggtc gagacgccct cgcgccggat
     5701 cgtggtcgac accgggcttg gcaatgacaa ggaaggccg
//
LOCUS       DASWGM010000086         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_838109,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000086 DASWGM010000000
VERSION     DASWGM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838109"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VGA15_02740"
     CDS             <1..1303
                     /locus_tag="VGA15_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX9586647.1"
                     /translation="WGYDGVLWYAPDSVYGRPDDLKALIDEAHLRGLMVFLDVVYNHF
                     GPEGNYLGRYAPGFFTAAQTPWGSAIDYRVPQVRAFAIQNALHWLGEYRFDGLRLDAV
                     HAIPDLGEIPMLHDLSVAAGKLAAETGRHIHLVLENDDNTASALDAGQDPPRGKYRAQ
                     WNDDYHHAWHVLLTRESHGYYGDYQRSPLHDIARALGSGFVYQGEASAHRGGRLRGEP
                     SGTLAPTAFVNFLQNHDQIGNRAFGDRLETTVSAVAIEAAVAITLLAPAVPMLFMGEE
                     WGSKAPFPFFCDFEGELADAVRNGRRKEFAGAYAKYGDGIPDPLDPLTFRSAILDWDS
                     RNEQAGQRRLALVRDLLAIRRREIVPRLAGAAFGHARAADNGLLTADWRMGDGATLGL
                     LANLSDQAIDHQEKKTGTPIWGGETSGLVPPWSVFWRLEAR"
     gene            1303..>2579
                     /locus_tag="VGA15_02745"
     CDS             1303..>2579
                     /locus_tag="VGA15_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9586648.1"
                     /translation="MRAAIPIATYRLQLTASFDFDAAAAIAPYLKALGITHLYASPFM
                     KARKGSAHGYDIVDHTKINPELGGDAGFERLSGALKQYDLGLILDFVPNHVGVHFADN
                     PWWLDVLEWGPASPHAVSFDIDWELLPYRARGGVLLPIIGSSYRQALEKGEIELRYDA
                     DGGSFSAWYFEHRLPIAPERYGEILGAILREAGAEDSAAGQRILALASRYKGLHHPNR
                     KEAPAFKAELRAIAGGAEIIARGLDAYRAGPDRAAQILALHHLLERQHYRLGHWRLAS
                     SDINYRRFFDINTLAGLRVEDAGTFDAIHRLVKTLIAENKLQGLRLDHIDGLRDPAQY
                     FQRLRRLIRDAQGRDAKPFYMAIEKILGEHEKLRRFAGVDGTTGYEWLNTITQVLIDG
                     KGLKPLDEVWRQVSNMSPKLEPVLKDAKRRVLET"
BASE COUNT          489 a          807 c          803 g          480 t
ORIGIN      
        1 ttggggctat gacggcgtgc tgtggtacgc gcccgacagc gtctatggac ggcccgacga
       61 tctcaaagcc ctgatcgacg aagcgcatct gcgcgggctg atggtatttc tcgacgtggt
      121 ctataaccat tttggtcccg aaggaaatta tctcggccga tacgcgccag gcttcttcac
      181 agcggcgcag acgccctggg gcagcgcgat cgattaccgg gtgccgcagg tgcgtgcctt
      241 tgcgattcag aatgcgctgc attggctggg tgagtatcgt ttcgacgggc tgcggctgga
      301 tgcggtccat gccatccccg atctgggtga aattccgatg ctgcatgatc tcagcgtcgc
      361 ggcggggaag cttgcggccg aaaccggccg gcacatccat ctggtgctcg agaatgacga
      421 caacacagcc agtgcgctgg atgccggcca ggacccgccg cgcggcaaat atcgcgcgca
      481 gtggaacgac gattatcatc acgcctggca cgtgctgcta acccgggaat cccacggcta
      541 ctatggtgac tatcagcgtt caccgcttca cgacatcgca cgcgccctcg gctccggctt
      601 tgtctatcag ggcgaagcct ccgcgcatcg cggcgggcga ttgcgcggcg agccaagcgg
      661 cacactcgct cccaccgcgt tcgtcaattt cctccaaaac cacgatcaga tcggtaaccg
      721 cgcattcggc gaccggctcg aaaccactgt aagtgctgtc gcgatcgaag ccgccgtggc
      781 gatcacgcta ttggcgccgg ccgtcccgat gctgttcatg ggcgaagaat ggggctcgaa
      841 agcaccgttc ccgttcttct gcgatttcga gggcgagctt gcggatgccg tacgcaacgg
      901 ccggcgcaag gagtttgccg gcgcctatgc gaagtacggc gatgggattc ccgacccgct
      961 ggatcccctg acgtttcgat ccgccatcct cgattgggac tcgcgcaacg aacaggcggg
     1021 acagcggcgg ctggcgctgg tgcgagactt gctcgcgata cgccggcgcg agatcgttcc
     1081 gcggctggcg ggcgcggcgt tcggccatgc gcgtgcggcg gacaatggtt tgctgacggc
     1141 cgactggcgc atgggtgacg gcgcgacgct tggcctcttg gccaatctgt cggaccaggc
     1201 cattgatcat caagaaaaga aaacgggcac cccgatctgg ggcggcgaga ccagcggcct
     1261 cgttccgcca tggtcggtgt tctggcgcct ggaagcgcga taatgcgtgc cgccattccg
     1321 atcgcaacct accgcctgca actcaccgcg agttttgatt tcgacgccgc tgccgccatc
     1381 gctccctatc tgaaagcgct gggtatcacg cacctctatg cgtcgccctt catgaaggcg
     1441 cgcaagggca gcgcccacgg ctatgacatc gtcgatcaca ccaaaatcaa tccggaactc
     1501 ggcggcgacg ccggcttcga gcgattaagc ggcgcactga agcagtacga cctcggcctt
     1561 attctcgatt tcgtgcccaa ccatgtcggc gtgcatttcg ccgacaatcc atggtggctc
     1621 gacgtgctgg aatgggggcc ggcctcgcca catgcggtgt cgttcgacat cgactgggag
     1681 cttctgccgt accgggcgcg tggcggcgtg ctgttgccta tcatcggctc gtcctatcgg
     1741 caggcgctgg aaaagggcga gatcgaactg cgctacgatg ccgatggagg cagcttttcc
     1801 gcgtggtatt tcgaacatcg gctgccgatc gcgcctgagc gctacggcga gatcctgggc
     1861 gcgatcctga gagaagccgg ggctgaagac agcgcggctg gacaacgcat cctcgcgctc
     1921 gcctcgcgct acaagggatt gcatcatccc aatcggaaag aagcgcccgc tttcaaggcg
     1981 gagctaagag ccatcgcggg gggcgctgaa attatcgcgc gcgggctcga tgcctaccgc
     2041 gcgggaccgg atcgcgccgc gcagatcctt gccctgcatc atctgctgga acgccagcac
     2101 tacaggctgg gccattggcg attagcctcc agcgacatca actatcgacg cttcttcgac
     2161 atcaacacgc tggcgggctt gcgggtcgag gatgccggaa cattcgacgc gatccatcgc
     2221 cttgtcaaaa cgctgatcgc cgaaaacaag ctgcaaggct tgcggctcga tcacatcgac
     2281 ggcctgcgcg atcccgcgca atattttcag cgcctgcgcc gcctgatccg cgacgcccag
     2341 ggacgagatg caaaaccgtt ttacatggcg atcgaaaaga ttcttggcga acatgaaaag
     2401 ctgcgtcgct tcgccggcgt cgacggtacc accggctacg aatggctgaa taccatcacc
     2461 caggtgctga tcgacggcaa aggcctcaag ccgctcgacg aggtgtggcg gcaagtcagc
     2521 aatatgtcgc ccaagttgga gccggtcctg aaggacgcca aacgccgggt tctcgaaac
//
LOCUS       DASWGM010000087         5173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2224002,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000087 DASWGM010000000
VERSION     DASWGM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2224002"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VGA15_02750"
     CDS             <1..559
                     /locus_tag="VGA15_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HEX9586649.1"
                     /translation="GLLTNPLRDRFGIPVRLNFYTVEELEKIVTRGARVLKIGMTPDG
                     ANEIARRARGTPRIAGRLLRRVRDFASAADAASIDRAIADNALSALEVDAAGLDAMDR
                     RYLTTIAMNYGGGPVGVETMAAALSEPRDAIEDIIEPFLIQCGYLQRTPRGRLLTSHA
                     FRHLGLAEPARDPVQFGLFGGGEEE"
     gene            613..807
                     /locus_tag="VGA15_02755"
     CDS             613..807
                     /locus_tag="VGA15_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9586650.1"
                     /translation="MRTTVSLDDELLATAERLTNVHENSALLREALKALIEREAARRL
                     ARLGGTAPRLKAPPRRRMNK"
     gene            981..1904
                     /locus_tag="VGA15_02760"
     CDS             981..1904
                     /locus_tag="VGA15_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9586651.1"
                     /translation="MISRRQFFRFLAGIGAFGASTAAYGFGIEPVLRLRVARYDITPP
                     RWPAGLQLKIAAIADLHACDPWMSLDHIQSIVERTNALDADLIVLLGDYVVGHRHVTR
                     AIPAGEWAPLLAGLKAPLGVHAILGNHDWWDDKTVQRDGRGPTKARRALEAAGIPVYE
                     NDVARLTKAGRPFWLAGLGDQLAYFPARRFRPVSRIGVDDLTATLAKVKDDAPVILLA
                     HEPDVAIRVPSRVSLQLSGHTHGGQVRLLGWSPVVPSRYGNRFAYGHARASCDVVVSG
                     GLGCSIMPFRLGVPPEIVLVTLGGSNPVVSS"
     gene            2033..2830
                     /locus_tag="VGA15_02765"
     CDS             2033..2830
                     /locus_tag="VGA15_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586652.1"
                     /translation="MFDLLSFQPSMIMRLCWQAFAVASIFFSTPSVAGPSYGSRLLEH
                     VRNFRETFTPPLIPVSDETRRREPDTTVFASMSGKCRTLKIAGRDFACRVVAFYQTEQ
                     GRANFTIVLDDPADNAHIVTFSGENGRKDQDDLYELSVDRMLLRSKDRPKVDGLPAPL
                     VELSTGTCKQLGNFAKGQVSSIACIAAGTNGKKYELQFETDGSPIRVMRVRETPVPTE
                     RRRAKQIEQFECRLKADAAKILPRDRTAYIIGCLGEDSRQPTTIDRQ"
     gene            complement(2894..4123)
                     /locus_tag="VGA15_02770"
     CDS             complement(2894..4123)
                     /locus_tag="VGA15_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9586653.1"
                     /translation="MTTPVRKLSLLAGAACCFLVGVTAAFPQPAPSTQAAPPTQAAPA
                     PPTPVPFDDALLRAANDLFSKAAVPPGDGKIELVIDPLIDGISGAQSTATRLMQERIV
                     ALVRKSYPRFEVRPFSSEALSRAPVVLIGTFTAINNAGAADGPRDAYRICLALADLRS
                     KSIVSKGVARARPEAIDVTPTPAFADTPVWAMDAATSMYIKACQGTKPGDPIDPVYVE
                     RIGTSSLISDAILEYDDRRYRESLAFYRTALKMTGGDQLRVRSGIYLANWKLNRRDDA
                     TDAFGSLVDYSLAGKKLSMRFLFKPGSTQFVDDPQISAPYPMWLSQIAVRATQRNTCL
                     QLVGHTSKTGPPQVNERLSVLRAQFIKDLLQSSSPRLADKLKATGVGFKENLIGTGRD
                     DASDAIDRRVDFNVSDC"
     gene            complement(4214..>5173)
                     /locus_tag="VGA15_02775"
     CDS             complement(4214..>5173)
                     /locus_tag="VGA15_02775"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9586654.1"
                     /translation="MLIAYSTQPNAVAADGTGRNSPFTAALVRELQVPGLEVATVFRR
                     VAVNVNRETAGAQTPELSVSLLGDFYLNPQESDISAWQKLGPSATADGLKTFISRFPT
                     SVMVDAARARLDAIEHASERERLAREYEEKERRLRQELEAAEAGYRKATSELAARRER
                     DERERLADAKSGQPPVAAAPPAPAAVSLGSTPEIAVRLPDDNERERQAKEQAAAAERE
                     RLASQVAAFEADKARLARERQALELAMSESLARAQAERERAEKRVAAETARLPVIALD
                     QPRTMAPPAPPRRTASTCQEINARAQLGDISDADRDTLKRGCH"
BASE COUNT          921 a         1626 c         1621 g         1005 t
ORIGIN      
        1 gggcctgttg accaatccgc tgcgcgaccg ttttggcatt ccggtgcggt tgaatttcta
       61 taccgtggag gaactggaaa agatcgtcac ccgcggcgcc cgcgtgctca agatcgggat
      121 gacgccggat ggcgccaacg agatcgcgcg ccgtgcccgc ggcacgccgc gcatcgcagg
      181 aaggctgtta cggcgggtgc gcgattttgc ctccgcggcc gatgccgctt cgatcgaccg
      241 cgccatcgcc gacaacgcgc tgagcgcatt ggaagtcgat gccgccgggc tcgacgccat
      301 ggaccggcgt tatctcacga ccattgccat gaattacggc ggcggcccgg tcggcgtgga
      361 gaccatggcg gcggcgctgt cggagccgcg cgatgccatc gaggacatca tcgagccgtt
      421 cctgattcag tgcggctatc tgcagcgcac cccgcgcggg cggctgttga cctcgcacgc
      481 gttccgccat ctcggtctcg ccgagcccgc ccgcgatccg gtgcagttcg gattgttcgg
      541 cggcggcgag gaagagtagg catggaaatc cacatcagat gatgcaatat ttcgtgtctt
      601 ggagaatatc acatgcgtac gacggtgtct ctcgacgatg aattgctcgc cacggccgag
      661 cgtttgacca atgttcacga gaactcggcc cttcttcggg aggcgctcaa ggcgctgatc
      721 gaacgcgagg ccgcgcgtcg gcttgcgcga cttggcggaa cagccccacg gctgaaggcg
      781 cccccgcgtc ggcgaatgaa taaatgattc ttcccgatac ctcagtttgg gttgaacatt
      841 ttcgtcgcgg tcaacagacg ccaattgctg ctaagcgacg agggcgacga cgaacgcgac
      901 gctgctgaat atctgcacaa acgcaaccca attccgctgc aatttctgat caaaaaaaga
      961 tgcatcacga atagccattc atgatttcac gccgccagtt ttttcgtttt cttgccggca
     1021 ttggcgcctt cggtgcgtca accgcggcat atgggttcgg catcgagccc gtgctgcggc
     1081 ttcgcgtcgc acgctacgac attaccccgc cgcgatggcc cgccggtttg cagctcaaga
     1141 tcgccgcgat cgccgatctt catgcctgtg atccctggat gtcgctggac cacatccagt
     1201 cgattgtcga acgcaccaat gcgctcgatg cggatctcat cgtgctgctc ggcgactatg
     1261 ttgtcggcca ccgccatgtg acgcgcgcca ttccggccgg cgaatgggcg ccgctgctcg
     1321 ccgggttgaa ggcgccgctc ggcgtgcatg cgatcctcgg caatcacgac tggtgggacg
     1381 acaagaccgt gcaacgcgac gggcggggac cgaccaaggc gcggcgcgcg ctcgaggccg
     1441 ccggcattcc ggtctacgaa aacgacgtcg cgcggctgac caaggcggga cgtccgttct
     1501 ggctcgccgg gctcggcgat caactggcgt attttccggc gcggcggttc cgccccgtca
     1561 gccggatcgg cgtcgacgat ctgacggcga cgctcgccaa ggtgaaggac gacgcgccgg
     1621 tcatcctgct cgcgcacgag cctgacgttg cgatacgcgt gccgtcgcgg gtctcgttgc
     1681 agctctccgg ccatacccat ggcggccagg tgcggctgct cggctggtcg ccggtggtgc
     1741 cgtcgcgcta cggcaaccga tttgcctatg gccatgcgcg ggcaagctgc gatgtcgtcg
     1801 tctccggcgg cctcggctgc agcatcatgc cgttccgcct cggcgtcccg ccggaaatcg
     1861 tgctggtgac gttgggcgga tcaaatccgg tcgtatcatc gtaaagccgg cgttcatcta
     1921 cgaaagtcga gggcccctcg cgattggttc ctcaaaaaat atccggtggc gaccgcgccg
     1981 gtattgacgt cgatcaatat tcccgcttga cccaattgat gttacacggc gcttgttcga
     2041 tcttttgtct tttcaaccga gcatgataat gcgcctctgc tggcaagcct tcgcggtcgc
     2101 aagtattttc ttctcaactc cgtcggttgc cgggccatct tatggcagcc gactcttgga
     2161 gcatgtccgg aatttcaggg agacattcac gccgcccctg attcctgtct cggatgaaac
     2221 cagacgacga gaaccggaca ccacggtatt cgcgtcaatg tcaggcaagt gcagaacact
     2281 caaaatcgcg gggcgcgatt tcgcgtgcag ggttgttgcg ttctaccaaa ccgaacaggg
     2341 ccgggccaat tttaccattg ttctcgacga tcccgctgac aatgctcaca tcgtgacgtt
     2401 ttcgggtgaa aatggccgga aggatcaaga cgatctatac gaactgtctg tcgaccggat
     2461 gctgctgaga tccaaggacc gaccgaaggt cgatggcctg ccggcgccgc tcgtcgaatt
     2521 gtcgacggga acatgcaagc agcttggaaa tttcgcgaag ggtcaggttt ccagcatcgc
     2581 ctgtatcgcg gcaggtacaa atggcaaaaa gtatgaattg cagttcgaaa ccgatggctc
     2641 gccaattcga gtgatgaggg tcagggaaac cccggtgcca acggaaaggc gccgcgccaa
     2701 gcagatcgag caattcgaat gccgtctcaa ggccgatgcg gcgaagatcc tgcccagaga
     2761 ccggacggcc tacattattg gatgcctggg agaagacagc cgacaaccca cgacaatcga
     2821 ccgtcaatga agcgataccg tcggacgatc gttgcgccag ctttgtgccg caacaagcga
     2881 tattcaaccg gtctcaacag tccgaaacgt tgaaatcgac ccggcggtcg atcgcatcgc
     2941 tggcgtcatc cctgccggtg ccaatcaggt tctccttaaa gccaacgccg gtcgctttca
     3001 acttgtcggc gagacggggc gagctgctct gcaggagatc cttgatgaat tgagcgcgca
     3061 gcactgacag ccgctcattg acctgtgggg gtcctgtctt cgaggtatgg ccgacgagtt
     3121 gaagacaggt attccgctgc gttgcccgca cggcgatctg actgagccac atcgggtagg
     3181 gcgcggagat ctgcgggtca tcgacgaact gggttgagcc gggcttgaac agaaaccgca
     3241 tcgacaattt cttacccgcc agactgtaat cgaccagact gccgaacgcg tcggtcgcgt
     3301 catccctgcg gttaagtttc cagttggcga gatagatacc ggagcggacg cgcagctgat
     3361 ctccgcccgt catcttgagc gcggtacggt aaaaagccag cgactcccga tagcgcctgt
     3421 cgtcgtattc caggatggcg tcgctgatca gcgacgaggt gccgatgcgt tcgacataga
     3481 ccgggtcgat cgggtcgccc ggcttggtgc cctgacaggc cttgatgtac atgctggtcg
     3541 cagcatccat ggcccagaca ggggtgtccg caaatgccgg cgtcggcgtc acatcgatcg
     3601 cctccggcct ggcgcgggcg acgcccttgg agacgatgct cttgctgcgc agatcggcca
     3661 gcgcaaggca aatccgatag gcgtcgcggg gtccgtccgc cgcgcccgca ttgttgatcg
     3721 cggtaaaggt accgatcagc acgaccggtg cgcgcgacaa cgcttcgctg gagaagggac
     3781 gcacctcgaa acgcgggtac gacttcctga ccagcgccac gatgcgctcc tgcatcaatc
     3841 gcgtcgccgt cgattgcgcg ccgctgatgc cgtcgatcag cgggtcgatc acgagttcga
     3901 tctttccgtc gcccggaggc accgcggctt tcgaaaacag atcattggcc gcccggagca
     3961 gcgcgtcgtc gaacggcacc ggtgtcggcg gcgccggcgc ggcttgcgtg ggtggtgcgg
     4021 cctgggtgct tggcgcgggc tgcgggaagg ccgccgttac gccgaccagg aaacagcagg
     4081 ctgcgcctgc gaggaggctc aactttcgca ccggcgtcgt catgcgtcgt ctccgtgccg
     4141 cggcaagctc cgacatgcaa atgccccgga ttgtcgaatc gccttgatgt ctcatttcag
     4201 tccctatccg cgatcagtgg cagccacgct tgagagtatc gcgatcggcg tcggagatgt
     4261 cgccaagctg agcgcgcgcg ttgatctcct ggcaggtcga tgcggtacgc cgcggcggcg
     4321 ccggcggcgc catcgttcgc ggctgatcga gtgctatgac cggcaagcgc gcggtttccg
     4381 ccgccactcg tttttccgcc cgctcgcgct cggcctgcgc cctggcaagg ctttccgaca
     4441 tcgcaagctc aagcgcctgg cgttcgcggg cgagccgggc cttgtcggcc tcgaatgcgg
     4501 caacctgcga tgccagccgc tcgcgctccg cggcggctgc ctgttccttc gcctgccgct
     4561 cgcgctcgtt gtcgtcgggg agccgaactg cgatctctgg agtgctgccc aaactgacgg
     4621 ccgcgggggc cggcggcgcg gcagcgaccg gaggctggcc gctcttggcg tcagccaatc
     4681 gctcgcgctc atcccgttcg cgtcgcgctg cgagctccga cgtggctttt cgataaccgg
     4741 cctcggcagc ttcgagctcc tgtcgcaggc gacgctcctt ttcctcatac tcgcgcgcca
     4801 gccgttcccg ttcgctggca tgctcgatgg cgtccaggcg cgcccgcgcc gcgtcaacca
     4861 tcacgctggt gggaaacctt gaaatgaatg tcttcaggcc gtcagcggtt gccgagggcc
     4921 ccagcttttg ccaggcgctg atatcgctct cctgaggatt gagatagaaa tcccccagca
     4981 gcgataccga cagttccgga gtctgtgcgc cggcggtctc gcgattcaca tttaccgcga
     5041 cacggcgaaa taccgtcgcg acctccagcc cgggaacctg aagttcgcgc accagtgccg
     5101 cggtgaaagg gctgttgcgc ccggtgccat cggcggcgac ggcgttgggt tgcgtcgaat
     5161 aggcgatcag cat
//
LOCUS       DASWGM010000088         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_838249,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000088 DASWGM010000000
VERSION     DASWGM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838249"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VGA15_02780"
     CDS             complement(<1..760)
                     /locus_tag="VGA15_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX9586655.1"
                     /translation="MSPIQPAQIASIFRYPVKGLSPEPLPSVTLSVGQTLPADRRYAI
                     ENGPSGFDPANPKWMVKAYFLMLQRDEWLAALHTHFDDASNVLTISQNGAIAAQGDLE
                     TADGRIAIERFFANNYAAAIKGPPKVLSSNGHSFSDIARKVVSIINLGSIAAIEDIVG
                     QPVHPLRFRANLYVRGWPAWHESELLDRTLGIGPVRLKVVKRIVRCAATNVDPETAAR
                     DLTIPHTLMRHLGHNECGIYAEVIAGGSIAVDDAI"
     gene            1090..>2597
                     /locus_tag="VGA15_02785"
     CDS             1090..>2597
                     /locus_tag="VGA15_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX9586656.1"
                     /translation="MNIEKYTERARGFIQSAQSLAMRDGHQQFSPLQMLKVLLDDSEG
                     LAGGLIDRAGGNSRAILKATEDALNKLPKVSGSGAGQVYLAPELARAFDAAEKAADKA
                     GDSFVTVERLLLGLTLEKDSDAGAILRKGGVTPQNLNAAIEALRKGRTADSATAENAY
                     DALKKYARDLTQAAREGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLALRILNGDVPESLKDKKLLSLDLGALIAGAKYRGEFEERLKAVLQEVTSAEGSII
                     LFIDEMHTLIGAGKADGAMDASNLLKPALARGELHCIGATTLDEYRKHVEKDAALARR
                     FQPIFVSEPTVEDTISILRGLKDKYEQHHGVRITDSALVAATTLSNRYITDRFLPDKA
                     IDLMDEAAARLKMQVDSKPEELDSMDREIIRLKIEQEALKKESDAGSKSRLQTLEKEL
                     AELEKRSADLTSRWSAEKNKLSNAQKLKSELDALRIELANAQRRGEYQRAGEL"
BASE COUNT          525 a          771 c          869 g          432 t
ORIGIN      
        1 cgatcgcatc gtcgacggca atcgagccgc cggcgatcac ctcggcatag atcccacatt
       61 cgttgtggcc gaggtgacgc atcagcgtgt gcgggatggt gaggtcgcgc gccgcggttt
      121 ccgggtcgac gttggtggcg gcgcagcgga cgatccgctt caccactttc agcctgaccg
      181 ggccaatgcc gagcgtccgg tcgagcagct cggactcgtg ccatgccggc cagccgcgga
      241 catagaggtt ggcgcggaat cgcaaggggt gaaccggttg gccgacgata tcctcgatag
      301 cggcgatgct gccgagattg atgatcgaga cgaccttccg ggcgatatcc gaaaaactgt
      361 gtccattgct tgagagaacc ttcggcggcc ccttgatcgc tgcagcgtag ttgttcgcga
      421 agaaccgctc gatggcgatg cggccgtcgg cagtttcgag atcgccctgg gccgcgatcg
      481 caccgttttg gctgatggtc aggacgttgc tcgcatcgtc gaaatgcgtg tgtagtgccg
      541 ccagccattc gtcccgctgc aacatcagga aataggcttt caccatccat ttggggttcg
      601 cgggatcaaa gccgctgggg ccgttttcga tggcgtagcg gcgatcggcg ggaagcgtct
      661 ggccgacgct gagcgtgacg cttggcagcg gttccggcga gaggcccttg accggatagc
      721 gaaagatgct ggcgatctgc gcaggctgga tgggggacat ccggcttctt aagggattcc
      781 cgggcggggc gccaccgcac acgaatttgt gaagccgcct cttccgtttc cgcaactcct
      841 tcccacatct ggcccaagcc ggtgcaatgc tggcgacaaa ccgccggctg gttgagaaat
      901 gtcaatgcgg tcggcggaaa cccaatgaag atgtcgagtc cgtgcctcgc gcatgatccg
      961 ccaaagtgtc ggcggtttgg cggccagatc atgcgcttct ttaagatttg gagcgcgatc
     1021 ggacgcaaaa ccggtttcca cttttgctga tcgcgctccg ggcggagcgg caggctgagg
     1081 gaaataacca tgaatatcga aaaatacacc gagcgtgcgc gcggttttat ccagtcggcg
     1141 caatcgctgg cgatgcgtga cggccaccag cagttctcgc cgctgcagat gctgaaagtg
     1201 ctgctggatg acagcgaggg cctcgccggc ggtctgatcg accgcgccgg aggcaattca
     1261 cgcgcgattc tgaaggcgac cgaggacgcg ctgaacaagc tgccaaaggt gtcgggcagc
     1321 ggcgccggac aggtctatct cgcgccggaa ctggcgcggg cgttcgatgc cgcggaaaag
     1381 gccgccgaca aggccggcga cagttttgtc accgtcgaac ggttgctgct cgggctgacg
     1441 ctcgagaagg acagcgacgc cggggccatc ttacggaaag gcggcgttac gccgcagaac
     1501 ctgaacgcgg cgatcgaagc cttgcgcaag ggccgcaccg cggacagtgc gacggccgag
     1561 aacgcctatg acgcgctgaa gaaatacgcc cgcgacctga cgcaggcggc gcgcgagggc
     1621 aagcttgacc cggtgatcgg gcgcgacgag gaaatccgcc gcaccattca ggtgctctcc
     1681 cgccgcacca agaacaaccc ggtgctgatc ggcgagcccg gcgtcggcaa gaccgcgatc
     1741 gtcgaaggcc tggcgctgcg gatcctcaat ggcgacgtgc cggaaagcct caaggacaag
     1801 aaactgctgt cgctcgatct cggcgcgctg attgcgggtg caaaataccg cggcgagttc
     1861 gaggagcggc tgaaggccgt gctgcaggaa gtgacctcgg cggagggcag catcatcctg
     1921 ttcatcgatg agatgcacac cctgatcggc gccggcaagg ctgacggcgc gatggatgcg
     1981 tcgaatttgc tcaagcccgc gctggcgcgc ggcgaattgc actgcatcgg cgcgaccacg
     2041 ctcgacgagt atcgtaagca cgtcgagaag gatgcggcgc tggcgcgccg gttccagccg
     2101 atcttcgtct cggagccgac cgtcgaggat accatctcga tcctgcgcgg cctgaaggac
     2161 aaatacgagc aacaccacgg cgtgcgtatt accgattccg cgctggtggc ggcgaccacg
     2221 ctgtcgaacc gctacatcac cgaccgtttc ctccccgaca aggcgatcga cctgatggac
     2281 gaggccgcgg cgcggctgaa gatgcaggtc gattccaagc cggaagaact cgattcgatg
     2341 gaccgggaaa tcatccggct gaagatcgag caggaggcgc tgaagaagga gagcgacgcc
     2401 ggttcgaaaa gccggctgca gacgctggaa aaggaacttg ccgaactcga aaagagatcg
     2461 gccgacctga cctcgcgctg gagtgcggag aagaacaagc tgtcgaacgc gcagaagctg
     2521 aagagcgagc tcgacgcact tcgcatcgaa ctcgccaacg cgcagcgccg cggcgaatat
     2581 cagcgcgccg gtgaact
//
LOCUS       DASWGM010000089         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_975838,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000089 DASWGM010000000
VERSION     DASWGM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975838"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..1219
                     /locus_tag="VGA15_02790"
     CDS             389..1219
                     /locus_tag="VGA15_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX9586657.1"
                     /translation="MTGLTHRQSEILNIARAFGRVMVEDLARRFEVSAQTIRKDLNDL
                     CDHRSLTRIHGGAIIASGVENLAYEARRFVAAEEKKAIGAAAAAQIPNGCSLFINIGT
                     TTEEVASALTSHEDLLVITNNLNVAMLLYRHPRIEVIVAGGAVRRADGAVIGSTAISL
                     IGQFKVDYAIIGASAIDEEGALLDFDYREVQAAQAIIANARSVMLVADSTKLRRSAPV
                     RIAHISQIQTFVTDAALPAGLANICHTRGIEVIEAMPKASVDIDENGVELPSTVTRLR
                     "
     gene            1524..3068
                     /gene="glpD"
                     /locus_tag="VGA15_02795"
     CDS             1524..3068
                     /gene="glpD"
                     /locus_tag="VGA15_02795"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX9586658.1"
                     /translation="MDRIFDLAIIGGGVNGCGIARDAAGRGNSVLLCEMNDLASGTSS
                     WSTKLVHGGLRYLEYYEFRLVREALIEREILWQIAPHIIRPLRFVLPHHDGLRPAWLL
                     RLGLFLYDHIGGRHLLPPTRSVDLTRDEVGKPLVPGRYVRGFEYSDCFVDDARLVVLT
                     ARDAADRGAEICTRSRAVEIRQVDGIWRVTVENTGSGERRTIHARVLVNAGGPWVEQV
                     LASGVGVNARAKVRLVQGSHIVMRKLYPHDRAYMFQNGDGRIVFVIPYQDDFTLIGTT
                     DRDFDGDPAKVKASTEEIKYLCDSVSEYLAKPVIPEDVVWTYAGVRPLYDDGASEAKA
                     ATRDYVFELDTPGGAPLLSIYGGKITTYRRLAEEALERLSPYLRSAKAREGWTAKSPL
                     PGGDMDVSAIPALSAELVRNYPFLNAAHANRLAHAYGTRAAKLLGHAKSMADLGQSFG
                     ATLTESEVRYLMSSEWACTAEDIVWRRSKLGLRLSPAEIAAIDDWIAAHHVSLERPLL
                     EAGRRP"
     gene            3065..>3375
                     /locus_tag="VGA15_02800"
     CDS             3065..>3375
                     /locus_tag="VGA15_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9586659.1"
                     /translation="MTVTLENVTRTVDGIPTIRDVSLTLERGTLSVLLGPTLSGKTSI
                     MRLLAGLDKPTTGRVAVDGKDVTGFDVRQRSVAMVYQQFINYPSMTVYDNIASPLRVQ
                     G"
BASE COUNT          621 a         1068 c         1045 g          641 t
ORIGIN      
        1 cggatctgat cgcggtcctc catcgtcttg atgttccagg tgaaggcaac aaggtcgcgc
       61 cagtagcatt ccgaagcgaa cattcccacg gcggcgtcga gatcgccggc caccagcgcg
      121 gcttcgaatt tgtcgagaaa aacctggacg cgcgcggtta gcgtggtgtc gagcatgatt
      181 tcctcccgaa ggtctttttt tcagttcttc tgctgaaagt agcgcaagct tatcccaatt
      241 atgggacgaa tccacacttg caggtccgca cttgcagggg tccatttgct gcagtgcgta
      301 tggccaacag cagcacttcc tgccgcatgt tgaccggacg ccagaaccga cgcaccatga
      361 agggcgcgtc ttcgcccgag gtgccgtcat gaccggtctc acccaccgcc aatccgagat
      421 cctgaatatc gcccgcgcct ttggccgggt gatggtggag gatctcgccc ggcgttttga
      481 agtttcggcg cagaccatcc gcaaggatct gaacgacctg tgcgaccacc ggtcgctgac
      541 gcggattcat ggtggcgcca tcatcgcctc cggcgtcgag aacctcgcct acgaagcgcg
      601 ccgcttcgtc gccgccgagg agaagaaggc catcggtgcc gcggcggcgg cgcaaattcc
      661 caacggctgc tcgctgttca tcaacatcgg caccaccacg gaagaagtcg cgagcgcgct
      721 aacctcacac gaagacctgc tggtcatcac caacaacctc aacgtcgcga tgctgcttta
      781 tagacatccg cgcatcgagg tgatcgtggc cggcggtgcg gtgcgccgcg ccgacggtgc
      841 ggtgatcggc tcgaccgcga tcagcctgat cggccagttc aaggtcgatt acgccatcat
      901 cggcgcgtcc gcgatcgacg aggagggcgc gctgctggat ttcgattatc gcgaagtcca
      961 ggcggcgcaa gccatcattg ccaatgcgcg cagcgtgatg ctggtcgccg actcgacaaa
     1021 gctgcggcgc agcgcgccgg tccgcatcgc ccatatcagc cagatccaga cttttgtcac
     1081 cgacgcggca ttgccggcgg gacttgccaa catctgccac acccgcggca tcgaggtgat
     1141 cgaggcgatg ccgaaggcgt cggtcgatat tgatgaaaac ggcgttgaac ttccttcgac
     1201 ggtcacgcgg ctgaggtaga gaccatcccc gttatgctga ggatgtgagg caatctgttt
     1261 cgtgtcccgg acgcggtgca gcgttcttca cgctgctccg cagagccggg acccatggcg
     1321 atgcgcgggg aataacgatg ggccccggac cagcagcgca ccgcaagagc gctgcgcagc
     1381 atccggggca cgcactcctg atgcaactca cagccaccct tcgctaatcc ccctttttgc
     1441 ttgctttcac atttggttgt gatttattcc aaaccgaaag caaaatcgcc aaagtgaact
     1501 ggcgaacgcc cggggaaacg tctttggacc gaattttcga cctcgcgatc attgggggcg
     1561 gcgtcaatgg ctgcggcatc gcgcgcgatg cggcgggccg gggcaattct gttttgctgt
     1621 gcgaaatgaa tgacttggcg agcggaacgt cgtcgtggtc gacgaagctc gtgcatggcg
     1681 gcctgcgcta tctggaatat tacgagtttc ggctggtccg cgaggcgctg atcgagcgcg
     1741 agatcctctg gcaaatcgcg ccccacatca ttcggccgct tcgcttcgtg ctgccgcatc
     1801 atgacggctt gcgcccggcc tggctgctgc ggctcggtct ttttctgtat gaccatatcg
     1861 gcggccggca tctgctgccg ccgacgcgct cggtcgatct gacgcgcgac gaggtcggaa
     1921 aacccctggt tcccggccgc tatgtcaggg gctttgaata ttcggactgt ttcgtcgatg
     1981 acgctcgcct ggtggtgctc acggcgcggg atgcggcgga tcgtggcgcc gaaatctgca
     2041 cccgttcccg cgcggtcgaa atccggcagg tcgatggcat ctggcgggtc accgtcgaaa
     2101 acaccgggag cggcgagcgc cgcactatcc acgcccgcgt gctggtcaac gccggcgggc
     2161 cgtgggtcga acaggtgctg gcgtcgggcg tcggcgtcaa cgccagagca aaagtgcggc
     2221 tggtgcaggg ctcccacatc gtgatgcgaa aactgtatcc gcatgaccgc gcctacatgt
     2281 tccagaacgg cgacggccgc attgtcttcg tcattcccta ccaggacgac tttaccctca
     2341 tcggcaccac ggatcgcgat ttcgatggcg acccggccaa ggtcaaggcg tcgaccgagg
     2401 aaataaaata cctctgcgat tccgtcagtg aatatctggc caaaccggtg atacccgagg
     2461 acgtggtctg gacctatgcc ggggttcggc cgctgtatga cgacggcgcc agcgaagcca
     2521 aggccgctac ccgcgactat gtgttcgagc tcgacacccc cggcggcgcg ccgctgctgt
     2581 cgatctacgg cggcaagatc acgacctatc ggcgcctcgc cgaagaggcg ctggagcggc
     2641 tttcgcccta tctgcgcagc gccaaggccc gcgagggctg gaccgcgaaa tcgccgctgc
     2701 ccggcggcga catggacgtc tcggcgatcc ccgcgctatc ggcggaactg gtgcgcaact
     2761 atccgttcct caacgcggcg catgccaacc ggctggcgca tgcctacggc acccgcgccg
     2821 caaaactgct cggccatgcg aaatcgatgg ccgatctcgg tcagtcgttc ggcgccacct
     2881 tgacggagag cgaggtccgg tacctgatgt cgtcggagtg ggcttgcacc gccgaggaca
     2941 tcgtatggcg gcgatccaag ctcggattgc gattgtcacc tgccgaaatt gccgccatcg
     3001 acgactggat tgcggcccat catgtttccc ttgaacgccc cttgctggaa gcgggaaggc
     3061 ggccatgacc gttacgctgg aaaacgtcac acgaaccgtg gatggcattc cgaccattcg
     3121 cgatgtctcg ctgacgcttg aacgcggcac cttgagcgtg ctgctgggcc cgacattgtc
     3181 cggcaagacg tcgatcatgc ggctgctcgc cggcctcgac aagccgacca ccggccgcgt
     3241 cgcggtcgac ggcaaggatg tcaccggctt cgacgtgcgg cagcgctcgg tggcgatggt
     3301 ctatcagcag ttcatcaatt atccctcgat gacggtctat gacaacatcg cctcgccgct
     3361 gcgggtgcag ggcaa
//
LOCUS       DASWGM010000090         7467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1669636,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000090 DASWGM010000000
VERSION     DASWGM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669636"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VGA15_02805"
     CDS             complement(<1..1154)
                     /locus_tag="VGA15_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9586660.1"
                     /translation="MQDHTSPSSLENAIALQKYGVGQPVRRKEDDTLVRGKGKFTDDF
                     NLPGQAYAWIVRSSHAHGVIRGIDTIAARAMPGVLGVWTGTDLAPANYGPYTCGLPLK
                     SRDGTPLLQTNRTALMSDKVRYVGDPVAFVVAETLAQARDAGEAVVLDIDPLPAVTEP
                     AEAAKPGAPQLYDHIPNNVALDYHYGGTAKVEAAFAGAAHVTKLDITNTRVAVVAMEP
                     RAALASYDKASERYTIQVPTQGVAGNRQNLAKNILKVPNEKVHLLTANVGGSFGMKNI
                     NYPEYLCILHAAKALGRPVKWTDERSTSFLSDSHGRAQRIHCELALDAEGKFLAVKIS
                     GYGNLGAYITGVAPSPLSLNVGKNLASVYRTPLMSVDIKTVLTNTTLMGA"
     gene            1351..2421
                     /locus_tag="VGA15_02810"
     CDS             1351..2421
                     /locus_tag="VGA15_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX9586661.1"
                     /translation="MNDDTRLRDGLGPRASPQGPAAAGDGAQAGPTSGSSVYAALDLG
                     TNNCRLLIACPTDDGFRVVDSFSRIIRLGEGISATGSISDAAIERAITALSICRDKIQ
                     SRKARRLRLIATEACRAASNAKGFRDRVAAETGIQLEVINRETEAALAVIGCSPLLDP
                     KGRGAILFDIGGGSTELVRVERDPDQQNAVPRIKGWMSIPLGVVTLAEHFGGRVVTAQ
                     SYARMVQEVAQHVAPFAAEHGGDLADMHLLGTSGTVTTLAGVHLNLARYDRRRIDGVW
                     MSDADLTVTIKRLLCMSYQERANNNCISVERADLVLAGCAILDAIRQAFPLSRLRVAD
                     RGLREGMLVEMMREDGVLSGCR"
     gene            2457..3149
                     /locus_tag="VGA15_02815"
     CDS             2457..3149
                     /locus_tag="VGA15_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX9586662.1"
                     /translation="MAKDTTGRLRVTVKSGGRLKLSSKLWLERQLNDPYVAQAKRDGL
                     RSRAAYKLIEIDDKHHFLKRGITVVDLGAAPGGWSQIAAKRVGAADGKGKVVAIDLLE
                     MPEIAGVSFAQLDFLADGAPDKLLDMMGGRADVVLSDMAANTTGHRKTDQLRILGLVE
                     GAAAFAADVLNPGGTFVAKVFQSGADAALMTQLKHDFATVKHVKPASSRKDSSERYVL
                     AMGFRGLPKAPA"
     gene            3319..3768
                     /locus_tag="VGA15_02820"
     CDS             3319..3768
                     /locus_tag="VGA15_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586663.1"
                     /translation="MRFEDELRQISLTEGLQSLTSAPNQAAKIGVAPTKARESEDTKY
                     LWVIAAEDVPLALERNNTAKLQRGYLSHTNLTGGADAHSGGELWFTDASVLILNGGSG
                     RYPPRSEAELEQAALAFKAMGYKVANMGWDSETNTPARYLRGDPKWI"
     gene            3765..5177
                     /locus_tag="VGA15_02825"
     CDS             3765..5177
                     /locus_tag="VGA15_02825"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX9586664.1"
                     /translation="MSDLKRIFGDVGSAVSVEDSARASTQIFVRSQDHLALYKKGAKG
                     RKLTAWQAYQAFGIDKLEEVFNYGSAVLVFGHQEPASSLRKSREDRGLTVYDIAKATK
                     LKVNEITSAEDARKTNPIQLLEKLAMTLGLDETRLSVRPLTEDNERLGIRLRQLKNSN
                     PAFSPSTVVAFDEAAWVIQKQLLLSEWLGEKVNLSSLGFVPDSRYGDKQYPAWQYGYD
                     LARATRKLLGINQKEPIESLRTIVEIKLKIPLTHLSLPSQFAGATISAGPARGIAVNV
                     NGSNQNVWIRRATIAHELAHLLWDPDSRLNTLVVDTFEAMESQPPHIKEKEDDFVEAR
                     ANAFAIEFLAPQEYALGLFETKPDMPSGLRAVMDYFGVSFTSAKFQIWNAKKRAIALD
                     TFSVQDVDPTDEWKGRESFTNDFFKPQSVPISRRGIFAKYVVESLSRKLISDDSAASF
                     LGCTEQELKNNLDTIKSIFR"
     gene            complement(5341..5721)
                     /locus_tag="VGA15_02830"
     CDS             complement(5341..5721)
                     /locus_tag="VGA15_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9586665.1"
                     /translation="MTTELKPIRTKTDYKAALAEVERLWGTKSGTPKGDRLDVLATLI
                     DAYEARRYPMDPPDPIEAIQFRMEQLGLTRKDLEPLIGTRARVAEVMNRKRSLSIDMI
                     RRLHQHLGISADVLIRPARENAAA"
     gene            complement(5711..6034)
                     /locus_tag="VGA15_02835"
     CDS             complement(5711..6034)
                     /locus_tag="VGA15_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEX9586666.1"
                     /translation="MRIIARRTLRQFVEGLSASKDRLAVKAALDAWFDEASKAAWKNT
                     ADVKRRYATASIVSAERIVFNIKGNDYRLVVAVDFEKAIIWIKWIGTHKAYDKIDVTK
                     VRHDN"
     gene            complement(6122..>7467)
                     /locus_tag="VGA15_02840"
     CDS             complement(6122..>7467)
                     /locus_tag="VGA15_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9586667.1"
                     /translation="KLAITSYLLSLAVFIPASGWTADRFGARMVFSIAVGVFIVGSIG
                     CALSSSVTDFVIARIVQGFGGAMMTPVGRLVLLRSIDKSALVNAMAWVTVPALVGPVI
                     GPPLGGFITTYLTWHWIFLINIPIGLLGIVMALRYIDPIRSEDPERFDLYGLVLAGIG
                     LGGIAFGLSIAGLNLVPWTVVIALVAIGTISMTLYVIHARRTASPVLDFALLRLPTMR
                     ASIIGGFLFRLGIGSLPFLLPLLMQVGFGLSPLRSGLVTFASAVGAMGMKTLAARIIR
                     TFGFRNIMTINAVVSSIFLAACALFTIATPLLLIMILLVVGGFFRSLQFTAINTLAYA
                     EVEPAQMSRATTLVSVNQQLAVSAGVAVGAFSVETTMLVHHVSELSAADFAPAFIVVA
                     IISTISVYFFYEMPADAGHQVSGRGVVVVPSSEPDAEPEETSAAETANARDQRLG"
BASE COUNT         1578 a         2150 c         2276 g         1463 t
ORIGIN      
        1 taggcgccca tcagcgtggt gttggtcagc acggtcttga tgtcgacgct catcagcggc
       61 gtgcggtaga cgctggcgag gttcttgccg acgttcaggg ataacggact tggcgcgacg
      121 ccggtaatat aggcgccgag attgccgtag cctgaaatct tgacggcgag gaacttgcct
      181 tcggcatcga gcgcgagctc gcagtgaatc cgttgcgcgc ggccatggct gtcggacaga
      241 aagctggtcg agcgctcgtc ggtccatttc accggacggc ctagcgcctt cgcggcatgc
      301 aggatgcaaa gatattcggg atagttgata ttcttcatgc cgaacgagcc gccgacattg
      361 gcggtcaaga ggtgaacctt ctcgttcgga accttcagga tgtttttcgc aaggttttgg
      421 cggttgccgg cgacgccctg ggtcggaacc tgaatcgtgt agcgctcgct cgctttgtcg
      481 taagaggcca gcgccgcccg cggttccatc gccacgacag ccacgcgggt gtttgtaata
      541 tcgagtttcg tgacgtgggc cgcgccggcg aaggcggcct cgaccttggc ggtgccgccg
      601 tagtgataat ccagcgccac gttgttgggg atgtgatcgt agagctgcgg cgcgccgggc
      661 tttgccgctt ccgcaggctc ggtcacggcg ggcaggggat cgatatcgag caccacggct
      721 tcgccggcat cgcgcgcctg cgcgagggtt tcggcaacca cgaaggccac gggatcgccg
      781 acatagcgca ccttgtcgct catcagcgcg gtgcggttgg tctgcaacag cggcgtgccg
      841 tcgcggctct tcagcggcag gccgcaggta taggggccat aattcgcagg cgcgaggtcg
      901 gtgccggtcc agaccccgag cacgccgggc atcgccctgg cggctatcgt gtcgatgccg
      961 cgaatcacgc cgtgggcatg gctggagcgc acgatccagg cgtaggcctg gcccggcaga
     1021 ttgaagtcgt cggtaaattt gcccttgccg cgcaccagcg tgtcgtcttc cttgcggcgg
     1081 actggctgcc cgactccgta tttttgcagt gcgatagcgt tctcgagcga ggacggagac
     1141 gtgtgatctt gcattgaaag atacccaaaa tgccggattt tgcgcgattt cgcggcttgt
     1201 tatcgagata acgcacctat atccgaacga caacgcccga ttgggcatgg tcccatgttg
     1261 gcggctgttg cgcgccccag cggcgctgct aaagtttccg tgaacggaaa ttcctcgtcg
     1321 gccgcaatcg gccgcggaaa gacatttttg atgaacgatg acacgcggct tcgcgacggc
     1381 cttgggccgc gcgcaagccc gcaaggaccg gctgcggcgg gagacggcgc gcaagcgggg
     1441 ccgacatcgg gctccagcgt ttacgctgcg cttgatttgg gtaccaataa ttgccgcctg
     1501 ctgatcgcct gtccgaccga cgacggattt cgcgtggtcg attcgttctc gcggatcatc
     1561 cggctcggcg agggtatctc ggcgaccggc tcgatcagcg acgccgccat cgaacgcgcc
     1621 atcaccgcgc tcagcatttg tcgcgacaag atccagtcaa ggaaggcgag acgcctgcgg
     1681 ctgatcgcaa cggaagcctg ccgcgcggcg tcgaacgcca agggttttcg ggatcgggtt
     1741 gccgcggaaa ccggcatcca gcttgaagtg atcaatcgcg agaccgaagc cgcgctggcc
     1801 gtgatcggct gctcgccgct gctcgatcca aaagggcggg gggccatcct gttcgatatc
     1861 ggcggggggt cgaccgaact ggttcgggtc gagcgtgacc ccgaccagca aaatgcggta
     1921 ccgcgtatca aggggtggat gtctatacca ctcggcgtcg tcacattggc cgagcatttc
     1981 ggcggcaggg tggtcaccgc gcaatcctat gcgcggatgg tgcaggaggt cgcgcagcac
     2041 gtcgcgccgt tcgcggccga acacggcggc gatctggcgg atatgcacct gctcggaact
     2101 tctggaaccg tgaccacgct ggccggggtc catctcaatc tggcgcggta cgaccgccgc
     2161 cggatcgacg gcgtctggat gagtgatgcg gatttgacgg tgaccatcaa gcgcctgctt
     2221 tgcatgagct atcaggaacg cgccaacaac aattgcatca gcgtcgagcg cgccgacctc
     2281 gtgctggcgg gctgcgccat cctcgatgcg atccgccagg cgtttccgct gtcgcggctg
     2341 cgcgtcgccg accgcggcct gcgcgagggc atgctggtcg agatgatgcg cgaggacggg
     2401 gttttaagcg gttgccgata ggtttatcgg ccggcgatgt gctagagccc tgcatcatgg
     2461 cgaaagatac caccggacgg ctgcgggtca ccgtcaagag cggcggccgg ctaaaactgt
     2521 cgtcaaaact ctggctggag cggcagctca acgatcccta cgtggcgcag gccaagcggg
     2581 atggcttgcg ctcgcgcgcg gcctataagc tgatcgaaat cgacgacaag caccactttc
     2641 tcaagcgcgg catcacggtg gtcgatctcg gcgcagcacc cggtggctgg agccagatcg
     2701 cagccaaacg cgtcggcgcc gccgacggca agggcaaggt cgtcgccatc gatctattgg
     2761 agatgccgga aattgccggc gtcagctttg cgcagctcga ttttctcgct gacggcgccc
     2821 ccgacaagct gctcgacatg atgggtggcc gcgccgatgt ggtgctgtcc gatatggccg
     2881 ccaacaccac aggccatcgc aagaccgatc agctgcgcat tctggggctg gtggaaggcg
     2941 ctgccgcgtt cgccgccgac gtgctcaatc ccggcggcac cttcgtagca aaagtgtttc
     3001 agagcggcgc ggatgcggcg ctgatgacgc agctcaagca cgattttgcg actgtcaagc
     3061 acgtcaagcc ggcgtcgagc cggaaggatt cttccgagcg ctacgtgctg gcgatggggt
     3121 ttcggggtct gccaaaggcg ccggcctaag accaaccgtt acaagcgcta tcctcgtcac
     3181 aatcgtaaga gttttgtttg gaggagcgag aaaggtcgtc taatttaagc aaagctgggg
     3241 gtaacattgg ccggttcgac gcaaatactt gccgcataca ggaaaacatt cccaatccgg
     3301 tcgcccgatt tatcgagaat gagattcgag gacgaattgc ggcaaatttc gctaacagaa
     3361 ggtcttcaaa gccttacctc tgcgccaaac caagctgcga aaattggcgt agcaccgacg
     3421 aaggctaggg aatctgagga tacaaaatat ctatgggtta ttgcagcgga ggatgtccct
     3481 ttggctcttg agcgcaataa taccgcgaag ttgcagcgag ggtacctgtc gcacacaaat
     3541 cttacaggtg gtgccgacgc acacagcggt ggagagcttt ggtttaccga tgcttccgtt
     3601 ttgatcctaa atggtgggtc tggacgctac ccgccgcgat ccgaggcaga gttggaacaa
     3661 gctgctctgg cattcaaggc gatgggttac aaagtagcaa atatgggttg ggatagtgaa
     3721 acaaacactc cggcacgtta tttgagaggc gatcccaagt ggatatgagc gacttgaaaa
     3781 ggatatttgg agatgtcggc tctgcagtta gtgtggagga tagcgctcgt gcgtccacac
     3841 aaatatttgt taggagtcag gaccacctcg cactttataa aaagggcgcc aagggccgca
     3901 agttgactgc atggcaagca tatcaagcct ttggtatcga taaactcgaa gaggttttca
     3961 actacggatc ggccgtctta gtctttggac atcaagaacc ggcgagttct ctgcgcaaga
     4021 gtcgggagga ccgtggtctt accgtgtacg atattgcaaa agctacgaag ttaaaggtca
     4081 atgagatcac tagtgcggaa gatgctcgca agacaaaccc gatacaactt ctcgaaaaac
     4141 tcgcgatgac gctggggcta gacgaaacac gattgagtgt tcgtcctctt acggaagaca
     4201 acgaaagact cggcatacgc ctaaggcagc tgaagaactc caatcctgcg ttcagcccga
     4261 gtacggtagt cgcgttcgac gaagccgcgt gggtaattca aaagcaacta cttctttcgg
     4321 aatggcttgg tgaaaaagtg aatttgtcga gtctcggctt tgtacctgac tctcgttacg
     4381 gagataaaca atatcctgcg tggcagtatg gctacgatct tgcgcgcgcc acccgaaaac
     4441 tgctcggcat caatcagaaa gagcccatcg agagtcttcg gacaatcgtc gagataaagc
     4501 ttaaaattcc actcactcat ctctcactgc catctcaatt tgccggagct actatctcgg
     4561 ccggtccggc aagaggcatt gcagtcaacg taaacggatc aaatcaaaac gtatggataa
     4621 gacgagctac aattgcgcac gaactagcgc acttgctctg ggatccggat tctagattga
     4681 acaccctcgt cgtggatact ttcgaggcaa tggagtctca gccacctcac attaaggaga
     4741 aggaggacga tttcgtcgag gcgcgcgcaa acgcattcgc catcgaattt cttgctccgc
     4801 aggagtatgc actagggctc ttcgagacaa agcccgacat gccaagcggg ttgcgcgccg
     4861 tgatggacta tttcggtgtg agcttcacaa gtgcgaaatt tcagatatgg aacgcaaaaa
     4921 agcgcgcgat tgccttggat accttttcgg ttcaggacgt ggatcctacg gatgaatgga
     4981 agggccgcga atccttcacg aacgacttct tcaaacctca atccgttccc atctcgcggc
     5041 gcggtatctt cgcaaaatat gtcgtcgaat ctctttctcg gaagttgatt tctgatgaca
     5101 gcgcagcttc gtttttgggt tgcacggaac aggaactcaa aaataatctt gatacaataa
     5161 aaagtatctt ccgctaagtc agcaaactag cttcgtggct ttgctcgtcg caatgacggg
     5221 gaaagggtga gcgtggcccg gatggagcaa ggcaaaaccc atgcggtatc acgtcgttcc
     5281 tggatttcgt tctgatgtcc taacgctcgc gtatagccgc agcacggtta gattccgctg
     5341 tcacgccgct gcgttttcgc gtgccgggcg gataaggaca tcggcggaaa tgccgaggtg
     5401 ctgatgcagg cggcggatca tatcgatcga caggctgcgt tttcggttca tgacttccgc
     5461 gacgcgtgcg cgcgttccga tcagcggttc gaggtctttc cgggtcaggc caagctgctc
     5521 catcctgaac tggatcgctt cgatcggatc ggggggatcc atcggatagc gccgggcttc
     5581 ataggcgtcg atcagcgtcg ccagcacgtc gagccggtcg cccttcggag tgccactctt
     5641 cgtgccccac aagcgctcca cctcggcaag cgcggccttg tagtccgtct ttgtacggat
     5701 cggtttcaat tcagttgtca tgtcgcacct tggtcacatc gatcttgtca taggccttat
     5761 gggtgccaat ccatttgatc caaatgattg ccttctcaaa atcgacagcc accacaagac
     5821 gatagtcgtt acctttaatg ttaaagacaa tccgctccgc gctgacgatg ctcgccgtcg
     5881 cataacgacg ctttacatct gccgtattct tccaggccgc cttgctcgct tcgtcgaacc
     5941 aggcatccaa cgcagctttc acggccagcc gatccttgct ggcggaaagg ccctcgacga
     6001 actgtcgcaa cgttcggcgg gcgatgatcc tcattcgcct cttactagca tggtcccgtt
     6061 atgggagcaa gaagcgtgcg tgcgaccgtc tgcagcagcg gcctggctga ccggaaatat
     6121 ctcaccccag cctttgatcc cgcgcgttcg cggtctcggc ggcggaggtc tcctccggtt
     6181 cggcgtcggg ctccgacgag gggaccacga ccaccccgcg tcccgatacc tgatggccgg
     6241 catcggcggg catctcgtag aagaagtaca ccgagatcgt cgagatgatc gccaccacga
     6301 tgaacgccgg cgcgaaatcg gccgcgctca attcgctgac atggtgcacc agcatggtgg
     6361 tttccaccga gaacgcgccg acggcgacgc cggcggaaac cgccagttgc tggttgacgc
     6421 tgaccagcgt ggtggcgcgg ctcatctgcg ccggttcgac ttcggcatag gccagcgtgt
     6481 tgatggcggt gaactgcagc gagcgaaaaa agccgccgac caccagcagg atcatgatca
     6541 gcagcagcgg cgttgcgatc gtgaacagcg cgcaggcggc cagaaagatc gaactcacca
     6601 ccgcattgat ggtcatgatg ttgcggaacc cgaaggtgcg gatgatgcgc gccgccagcg
     6661 tcttcatgcc catggcgccg actgccgatg caaacgtcac cagccccgag cgcagcggcg
     6721 acaggccgaa tccgacctgc atcaacagcg gcagcagaaa cggcaacgag ccgatgccga
     6781 ggcggaacag aaagccgccg atgatgctgg cccgcatcgt cggcagccgc agcagcgcga
     6841 aatccagcac cggcgaagcg gttcgcctgg cgtggatcac ataaagcgtc atcgaaatgg
     6901 tgccgatcgc gaccagggcg atgacaacag tccatggcac cagattgagg ccggcgatcg
     6961 aaagtccgaa cgcgatgccg ccaagcccga tccccgccag caccagccca tagagatcga
     7021 agcgctcggg gtcctcgctg cggatcgggt cgatgtagcg cagcgccatc acgatgccga
     7081 gcagcccgat cggaatgttg atcaggaaga tccagtgcca ggtcaggtat gtcgtgatga
     7141 agccgccgag cggcggcccg atcaccggcc ctaccagcgc cggcaccgtg acccaggcca
     7201 tcgcgttgac cagcgcgctt ttgtcgatcg agcgcagcag caccagccgc cccaccgggg
     7261 tcatcatcgc gccgccaaaa ccctgcacga tgcgggcgat cacaaaatcg gtgacggagg
     7321 aggacagcgc gcagccgatc gagcccacga taaagacccc gacggcgatc gagaacacca
     7381 tccgcgcgcc aaaacgatcg gcggtccagc cgctcgccgg aataaatacc gccagcgaca
     7441 gcagatacga agtgatggcg agcttca
//
LOCUS       DASWGM010000091         4233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_144494,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000091 DASWGM010000000
VERSION     DASWGM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144494"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VGA15_02845"
     CDS             <1..1125
                     /locus_tag="VGA15_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEX9586668.1"
                     /translation="NTLPHAIRELAGLGLLQKLDDIAIRTDELYYLNRHGQEVWRESR
                     GIDAGHDVPQFSIHRGRLQSVIHRAVEERLGQDAIHTGCRLGAFAQDEGGVTAYFFDR
                     SGAHTKTVRGDILIGADGIHSRVRETLFPDEGPPCWNGLMLWRGARDWPVFLSGRSMI
                     VAGGLHAKVVVYPIAEGSSPASRLTNWAVLVKVGDGNAPPPRREDWSRPGKREELMPH
                     VARFSVPYVDVPGLISATPEFYEYPTCDRDPLPYWSSGRVTLLGDAAHPMYPVGSNGA
                     SQAILDARCLADALVGSEHPRQALIAYEQKRLPMTAEIVRSNRRGGPEGVIDAVEQLA
                     PDGFDNVENVLSYSQREAIVRGYASKAGFAAVPGLTAVRA"
     gene            complement(2143..2598)
                     /locus_tag="VGA15_02850"
     CDS             complement(2143..2598)
                     /locus_tag="VGA15_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9586669.1"
                     /translation="MKSEITGITRANEGMQGISWNILGQTYVPKNCTEHSFSWHATLP
                     SGTFVPPHIHPDQDEYLYMLEGKLDFMLGGAEAQATPGDLIRLGMGVPHGIFNKSDQT
                     AKVLFWVSPTKKLYDLFWGLHNMKEQKPEDVVAMAAEHNIHFLPPPPGA"
     gene            complement(2709..3884)
                     /locus_tag="VGA15_02855"
     CDS             complement(2709..3884)
                     /locus_tag="VGA15_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586670.1"
                     /translation="MKQQLKLAGLAVLFGLAAGQAVAQEKLKIGVIVTLSGPAAALGS
                     QVRDGFALAIKDLGGKMSGRDVEVVAVDDELKPDGAVTKVKGLLERDKVDFVVGPIFS
                     NILQAIHKPVTDSKTFLISPNAGPSSYAGKNCSPFFYVTSYQNDQVHEILGKVAQDRG
                     YKRVYVLVPNYQAGKDSAAGFKLDYKGEIVEESYMPLGTLDFQVELSKIASLKPDAVF
                     TFMPGGMGVGLVKQYRQAGLADQIPVLSAFTVDESTLPAQQDAAVGMFGGANWAPNLD
                     NPQNKKFVSAYEATYNSVPGTYAMQGYDAAMLIDSAVKAVKGDLTDKEAVTAALKKAD
                     FTSLRGGFKFNTNGYPIQDFYLTKVAKRPDGKFQTEIVEKVFENYGDRYAKDCTPGN"
BASE COUNT          807 a         1298 c         1270 g          858 t
ORIGIN      
        1 aatacgctgc cgcatgcgat ccgcgaactc gccggcctcg gcctgttgca aaagctcgac
       61 gacatcgcga tacgcaccga cgaactttat tatctcaacc gccatggcca ggaagtgtgg
      121 cgcgaatccc gcggcatcga cgccggtcac gacgtgccgc aattctcgat tcatcgcggc
      181 cggctgcaga gcgtaatcca ccgcgccgtc gaggagcggc tgggacagga tgcgattcac
      241 accggctgca ggcttggggc cttcgcccag gacgaaggcg gcgtcaccgc gtatttcttc
      301 gatcgttcgg gtgcgcacac caagaccgtg cgcggcgata tcctgatcgg cgccgacggc
      361 attcattcgc gcgtgcgaga gacgctgttt ccggatgaag ggccgccgtg ctggaacggg
      421 ctgatgctgt ggcgtggcgc gcgggactgg ccggtgtttc tgagcggccg ttcgatgatc
      481 gtcgccggcg ggctgcatgc caaggtggtg gtgtacccca ttgcggaggg atcgagcccg
      541 gcgagccgcc tgaccaactg ggcggtgctg gtgaaagtcg gcgacggcaa tgccccgccg
      601 ccccgtcgcg aggactggtc gcggcctggc aagcgcgagg aactgatgcc gcacgttgcg
      661 cggttctcgg tgccctatgt cgacgtgccg ggcctgattt cggcgacgcc cgaattctac
      721 gaatatccga cctgcgaccg cgaccccttg ccgtattggt ccagcggccg cgtgacgctg
      781 ctcggcgatg ccgcgcatcc gatgtatccg gtggggtcga atggcgcctc gcaggcgatc
      841 ctcgatgcgc gctgtctggc cgacgcgttg gtcggttccg agcatccgcg ccaggcgttg
      901 atcgcgtacg agcagaagcg attgccgatg accgcggaga tcgtgcgctc gaaccgccgc
      961 ggcggcccgg aaggcgtgat cgacgcggtc gagcaattgg cgcccgatgg cttcgataat
     1021 gtcgagaacg tgctgagcta ttcccagcgc gaggccatcg tgcgcggcta tgccagcaag
     1081 gccggctttg ccgccgtgcc ggggcttacg gcggtgcggg cgtagctaac caatcgtcat
     1141 tgtgaggagc caccgggtct cgccttcggc gagcccgatg acaggctccg cgacgaagca
     1201 atccatcgaa gtcgttggca aaggcgcgcg tggctgctcc acaaagaccc gtcatacccc
     1261 gcgaaagcgg ggtatccagt atgccgcggc ttctcgattc catcactgac gtctcggagt
     1321 actggatcac ccgctttcgc gggtgatgac agctgagggc gtggcgcgcc cgtcctcaat
     1381 gcattgccac cgattcaatt ttcaaacagc caaaacaatc gcagatacga cttcgcgatc
     1441 tcgcggcgcg ttgcgcccga gttgtgcatg aacctttcgc cccctagagg gcgtggggaa
     1501 tgccgggtgc ccgctgcacc cgcggcctcg tgtgcacttt gcattggtag aaagcacacg
     1561 agtaacaacg agtacaccgg aacacccggc attcccgcac gcaatggttt taacggctta
     1621 tgtcgtgctc tccccggtga ccgggctctt ttgccaccgt catccgcgga gtaaggtttt
     1681 gtctaagccc ggttgggccg acgcaactcc gcgaacttga cgccagcgtc ggggcgtcag
     1741 gaccacacga ttttgccgtc cgcggcaaca tctctcgtca gcgtgccggt gatcgctcac
     1801 ggggtcagaa gaacccgccc tgccgatccc atcgcgcgca aaacgctgcc gcgtccaccg
     1861 catcccgtgc cctacgtccg tgacgatcgc gaaacgcccc tctgtgtggg acgggataac
     1921 gagggatata gagctgattt gggtcaggcg ggaagggaat tatttttgca aatgggactg
     1981 gactgggcaa atcacattga tctgtttcaa caatttccgc cgtgcgcgca cgaggatgta
     2041 ccggcgtagc ccggatgcag cgaagcgcaa tccgggattg tgatatcagc gagtgacggc
     2101 cccggatttc gctgcgctgc atccgggcta cgcgaaatac cctcacgcgc ccggcggcgg
     2161 cggcaggaag tggatgttgt gttccgcggc catggccacg acgtcctcgg gcttctgctc
     2221 cttcatgttg tgaaggcccc agaacagatc gtacagcttt ttggtcggcg atacccagaa
     2281 cagcactttc gcggtctgat cggatttgtt gaagataccg tggggaacgc ccatcccgag
     2341 ccgtatcaga tcacccgggg tcgcctgggc ctccgcgccg cccagcatga aatcgagttt
     2401 tccctccagc atatagagat attcgtcctg gtccggatgg atatgcggcg gcacgaaagt
     2461 gcctgatggc aacgtcgcat gccaggagaa ggaatgctcg gtgcagtttt tcggcacata
     2521 ggtctggccc agaatattcc aggaaattcc ctgcatgccc tcgttggccc gggtgatgcc
     2581 ggtgatctcg cttttcatcg ccttgtttcc ttccgatcgt tatttggacg cgttttcttc
     2641 acgcgaaccg gtatccaccc ccggatcaag tccgagggca tgcttcgctc gaaaacgctt
     2701 tggtttgctt agttgcccgg cgtgcaatcc ttggcgtaac ggtcgccgta attctcgaac
     2761 accttctcga caatctcggt ctggaatttg ccatcggggc gttttgcgac cttggtgaga
     2821 taaaagtcct ggatcggata gccgttggta ttgaacttga atccgccgcg cagcgaggtg
     2881 aagtcggcct tcttcagtgc ggccgtcaca gcctccttgt cagtgaggtc acccttcact
     2941 gccttgacgg cgctgtcgat cagcatggcc gcatcatagc cctgcatggc ataggtgccg
     3001 ggcacgctgt tgtaggtagc ttcataggcg ctgacgaatt tcttgttctg cggattgtcg
     3061 agattcggcg cccagttcgc gccgccgaac atgccgaccg ccgcatcctg ctgcgccggc
     3121 agcgtcgatt catcgaccgt gaacgccgac aggaccggaa tttgatctgc aaggccggcc
     3181 tgcctgtact gcttcaccag gcctacgccc atgccgccag gcatgaaggt gaacacggcg
     3241 tcgggcttca gcgacgcgat cttggagagt tcaacctgga aatccagggt gccgagcggc
     3301 atgtaggact cctcgacgat ttcacccttg tagtcgagct tgaatccggc ggcggaatcc
     3361 ttgccggcct gatagttcgg caccaggaca taaacgcgct tgtatcctcg gtcctgggcg
     3421 accttgccga ggatctcatg gacctgatcg ttctggtagg aggtaacata gaaaaacggg
     3481 ctgcagttct tgccggcgta gctcgacggg ccggcattcg ggctgatcag gaaggtcttt
     3541 gaatccgtca ccggcttgtg gatcgcctgc aggatattgg agaagatcgg ccccaccacg
     3601 aaatccacct tgtcgcgctc gagcaggccc ttgaccttgg tcaccgcgcc gtccggcttg
     3661 agttcgtcat cgaccgccac gacctcgaca tcccggccac tcatcttgcc gccgagatcc
     3721 ttgatcgcga gcgcaaaacc gtcgcggacc tgcgagccga gtgctgccgc cggaccggat
     3781 aaggtcacga tcacgccgat cttgagcttc tcctgcgcga cggcctgacc ggccgcgaga
     3841 ccaaacaaaa ccgccagtcc ggccaacttc aactgctgct tcatgactta atcccccgct
     3901 acaccaactc aaatccgatc tttcgaggct tgagcttatt ctgatgatgg ctgtcgccgc
     3961 aagcaaccca tcagcaacgc caagcctcac aagcaagcct tgcacgtcca atatgcaagc
     4021 cggcaatatg ggagccgcca atatgcaagc cgcgcgccaa ttgtttgaaa cctaaagaaa
     4081 tccggaaaca tcaaaattat cggttttgac ttgcagcatc gcctaaacta tttgaaggtc
     4141 gacatcaaag ggcgaaaaca cccggcaggc gtattgcatc atgatccttg attccgagac
     4201 gaaggccgtc gaactgccgg aagaccatgg cga
//
LOCUS       DASWGM010000092         4444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_839122,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000092 DASWGM010000000
VERSION     DASWGM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839122"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGA15_02860"
     CDS             complement(<1..435)
                     /locus_tag="VGA15_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586671.1"
                     /translation="MSASAGMPAEPVAALEPSEISCRDGAALALTVVRDGVITGEGPT
                     TKGRVHFSRELDADDAAWCARILTATAVEHEPVSRAEAEALFEINDAAAERSDDGRFD
                     DLLAKAVVHHAASESGLPVPSRTIALSPDTAIESWAPARAAGV"
     gene            804..1043
                     /locus_tag="VGA15_02865"
     CDS             804..1043
                     /locus_tag="VGA15_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586672.1"
                     /translation="MKAQLARFVIFEIAMLIALVIFMTMDVGWLSAALIVISVIAGIS
                     LSDMAFGRPGNNSARSGRTGPQSAPLTPASRQFHP"
     gene            1067..1726
                     /locus_tag="VGA15_02870"
     CDS             1067..1726
                     /locus_tag="VGA15_02870"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9586673.1"
                     /translation="MALQIGAVAPDFEAETTEGKIKFHDWIGNSWALLFSHPKDFTPV
                     CTTELGALAKLKPEFDKRGVKLMGLSVDPVDKHAKWSEDIKETQGAAPNYPMIGDTDF
                     KVSKLYDMLPASTTGDAATRTAADNQTVRNVFVIGPDKKIKLVLVYPMTTGRNFQEIL
                     RVIDSLQMTSKHRVATPADWKQGEDVIIAGSVTDDEAKTIYPQGWKSPKPYIRIVPQP
                     K"
     gene            complement(1914..2314)
                     /locus_tag="VGA15_02875"
                     /pseudo
     CDS             complement(1914..2314)
                     /locus_tag="VGA15_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665065.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
     gene            2535..3558
                     /locus_tag="VGA15_02880"
                     /pseudo
     CDS             2535..3558
                     /locus_tag="VGA15_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
     gene            3633..3911
                     /locus_tag="VGA15_02885"
     CDS             3633..3911
                     /locus_tag="VGA15_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586674.1"
                     /translation="MDDEKPDLEPMPDAMSSVTEAPKEAKPAVKKRRKTAAKKVAKKV
                     APKKKAKKAKKAAKKYAAKKSAKKTGKKAAKKTAKKAAKKKKAKKSKR"
     gene            complement(4047..4444)
                     /locus_tag="VGA15_02890"
                     /pseudo
     CDS             complement(4047..4444)
                     /locus_tag="VGA15_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503500.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT          972 a         1344 c         1337 g          791 t
ORIGIN      
        1 gacgccggca gccctggcag gcgcccagct ttcgatcgcg gtatccggcg acagcgcaat
       61 ggtgcgcgac ggaaccggca gaccggactc ggaggccgcg tggtgcacga cggccttggc
      121 cagcaaatcg tcgaaacgtc cgtcatcgct gcgctccgcg gcagcgtcat tgatctcaaa
      181 cagcgcctcg gcttccgcgc gactgaccgg ctcgtgctcg accgcggtcg cggtcaggat
      241 gcgcgcgcac caagcggcgt catcggcgtc cagctcacgg gaaaaatgaa cgcgaccttt
      301 ggtggtcggg ccttcgccgg tgatcacgcc atcgcgcacg acagtgaggg caagtgctgc
      361 gccatcgcgg caggagatct cagatggttc gagggcggcg acaggttcgg cgggcattcc
      421 ggcagaggca gacattggag ttctcttttg cttttttccg atcccagcaa ttccatgccg
      481 ggtgtgaacg agtggttaac gattcgaaac ttgcgtcacg acttttttaa acagttgctg
      541 gttggtcgct gtgacgtcgg tcacggttca gcgaggtcgc aaactggccc gaccatggtg
      601 cggcgcgcaa gaggtaatat cgggataatg atgcttttga agacattgtg atttttccgt
      661 cgcagttggg ggtattaaaa gatgctgcta gttgaactct caactggaga agcatgttct
      721 ccttttgcgg gggacgttcc attgagccgc cgtgcgccct atagtgaccc cgaatggcta
      781 tttcgcgggt tggaaagtgg gcgatgaagg ctcaactggc acggtttgtc attttcgaga
      841 tagcgatgct gatcgcgctt gttatcttca tgacaatgga tgttggctgg ctatccgccg
      901 cgctcatcgt catatcggtc atagccggca ttagccttag cgatatggcc tttggccgac
      961 ccggaaacaa ttcggctcga tctggaagaa cgggcccgca atcagcacct ctaacgccgg
     1021 catcccggca atttcatccc taacccgaac gataggagga tcgacgatgg cactccagat
     1081 tggcgccgtg gccccggatt tcgaggctga aaccaccgag ggcaagatca aattccacga
     1141 ctggatcggc aacagctggg cgctgttgtt ctcgcacccg aaggatttca ccccggtctg
     1201 caccaccgag cttggcgcgc tggccaagct gaagccggaa ttcgacaagc gcggcgtcaa
     1261 gctgatgggg ctcagcgtcg accccgtcga caagcacgcg aaatggtccg aggacatcaa
     1321 ggaaacccag ggtgctgcgc cgaactatcc gatgatcggc gataccgatt tcaaggtatc
     1381 aaagctgtac gacatgctgc cggcctcaac caccggcgac gccgctacgc gaaccgccgc
     1441 cgacaaccag accgtccgca acgtcttcgt catcgggccg gacaagaaga tcaagctggt
     1501 gctggtctat cccatgacga cggggcgcaa tttccaggaa atcctgcggg tgatcgattc
     1561 gctgcagatg acctcgaagc atcgtgtggc gacgcccgcc gactggaaac agggcgaaga
     1621 cgtcatcatc gcgggctccg tcaccgacga cgaggccaag acgatctatc cgcagggctg
     1681 gaaatcgcca aagccgtata tccggatcgt gccgcagccg aagtaacggc tcgacgtcgt
     1741 cgtcgccgac cgagcacctt gtagcgagac ttgtcatgcc cggccatagc agtctgtctt
     1801 gcgcagactg cgtaagctgg tctgcgatgc cgggcatcca cggctttagg gcgtgcccac
     1861 aagaaaaaga cgtagatggc cgggacaagc ccggccatga cggagcgacg gattcaaatt
     1921 ctcggcgtaa tccaattggc gatcatcagt ccgagcgtgg acgacaccgc cgtgacaaat
     1981 gcccccatgt gacatccatg atcaccaccg gccacgtcca atgcttcaat agcgatagcg
     2041 acgtcagttc aaaggtcgcg tagcaaaaga agccgaacag tgcgccatac agcagcgtcg
     2101 attgtgcggt ggccgttacc gaagcgctga caaagatcaa aattcccacc acgtaaagca
     2161 gctagaacag gatcgcgggc gcgggccgga tttcacgcag catgtcgccg acctgtgcgg
     2221 cgaaaaagcc cttggcgaca aatccaagaa acacaaaatc gatcgggacg aggacgatca
     2281 gcgtcgccag atagagaaca acataccggt tcacagccat ctccagcact ttttgcgcag
     2341 ttccaatgac tagagccttt tccgtttcga tagaatcgaa acggggctct agattcttga
     2401 tttgacgcgt tttcttgacg cgaaccggtg tccacttcgc ttgaaaacgc tttagatacg
     2461 catcaggccc gggagagttg caatgtcctg tccgtgcgtg gacccaacgt gtgccatgcg
     2521 cgttgaacca tctgatgccc ggccattccg ctcccccccg catcgtctgg tttcgcgacg
     2581 atctccgcct ctgcgatcat cctgccctgc atgtcgcgtc gaagacgggc gcgccggtga
     2641 tctgtcttta cgtctttgac gaagcgagcg acgcgctgtg cggacgaggc gcccgcccgc
     2701 ccacttggcg gcgcggcgcg ctggtggctg gcgccgtcgc tgcgggccct gcaaaccagc
     2761 ctcaaggcgg ttggcgcatc gctggtgctg cgcaaaggac ccgcagcacc gatcatcgcc
     2821 gggctggcat caggacggtc gccgcaccta cgcttcggcg agatcagtcc gcggcaggtc
     2881 tggcacgccg cccgctttgc cgcggccgag cgccctgccc ttgctggcga tgtcgataaa
     2941 tacttaagcg aactgggctg gcgcgaattc tgccgccacc tggtgtttga cgcgcccgac
     3001 ctggcgttgc tcaatctgca gccctcgttc gaagcgtttc catggaaacc tgacgacaag
     3061 gcgcttgtcg cgtggcaacg cggccagacc ggctaccccc tcgtcgatgc cgggatgcgt
     3121 cagctctggc acaccggagt catgcacaac cgggtccgga tggtggcggc gtcgttcctg
     3181 gtcaagcatc tgctgatcga ttggcgcgag ggagaaagat ggttttggga cacgctggtc
     3241 gatgccgacc ccggcagcaa tcccgccaac tggcaatggg tcgcgggctc aggcgccgat
     3301 gccgcaccct atttccgcgt gttcaatccg atcctgcaaa gcgagaggtt cgatcccgac
     3361 ggtgggtata tccggcgctg ggtgccggag ctcgcgcaat tgccgacggc cctgatccac
     3421 cagccatggc gcgcagcgcc gctcgagctt aaaggcgcgg gcgtggaact tggcacgacc
     3481 tatccggggc cgatcatcga tcacagagcg ggccgcggac gcgcgctgaa agcctatgcg
     3541 aaggtgcggg cgatgtgagt agacagcggc atccgcttta cacaccggca aatccggcta
     3601 tcgttgcgtc attaacaaca ctgggggacg atatggacga cgagaaaccc gatctggaac
     3661 cgatgcccga cgcgatgagc agcgttactg aagcgccgaa ggaagccaag ccggcggtca
     3721 aaaagcgcag gaagaccgcc gccaagaaag tcgcgaaaaa ggtagcgccg aagaagaaag
     3781 ccaagaaggc gaagaaggct gccaagaagt acgcggcgaa aaagtcggcc aagaaaaccg
     3841 gcaagaaagc cgccaagaaa accgcgaaga aagctgccaa gaaaaagaag gccaagaagt
     3901 cgaagcgcta gactctgatt cgacttcgcc gaatcagacc cgtcgctttt caaagcgtat
     3961 gcttaccggg atgatgccct gaccgcgccg cgcaagagcc tgtggataaa agtccggagg
     4021 cttttgttcc gggacttgag gcctcgtcag gctcgcgcgc gccggttgac cagaaacacc
     4081 gcggcggcgc agacaaacat gcccgcgatc gcgatcccat cgagccgctc gccgaacagc
     4141 acataggcca tgacagcggt caccgccggc accagataga acaggcttgc caccgagctc
     4201 gccgccgagc gctgatcagc cagtacaaca gtccgatcga tccgatcgac agcatcaccg
     4261 cgagccagat cagcgacagc atgaactcgc tggtccagtg caccaccaca ttgcgctcga
     4321 acaacgccgc acctgtcgcg aagaagatcg cgaccgcgac gaactgcacg agattgccgg
     4381 cacgccagtc gatgctgccg caatagcgcc tctggtacag cgtgcccaag gtgatgctga
     4441 ccag
//
LOCUS       DASWGM010000093         6668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1253518,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000093 DASWGM010000000
VERSION     DASWGM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253518"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1552
                     /locus_tag="VGA15_02895"
     CDS             173..1552
                     /locus_tag="VGA15_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX9586675.1"
                     /translation="MIHPDHNAKSRVAAISIFASTGMAAAKFVVGIAIGSLALISEAL
                     HSSVDVVATVITWLVVRVSDRPADHEHHYGHGKFESLSALGVIAMLYVLAGGILVESY
                     SRLREGAPPPTISAIPFIVLLIDIAVNFWRARALHRTARNTKSQALAADALHFASDVL
                     GSVAVMIGLGLSGLGYAWGDAVAAIGVAVVIALLGLRLARSTVETLLDRAPEGASEKA
                     IAAIRAVPGVVDVERLRMRMVGSTHFIDAIVQVPRTYPIDRVEEIKRRAQAAVSKVLD
                     DADLTFTAVPVARNNESIRERIMVIARNSGLAIHHVTVHDLGGKLTVSIDLEVDADMA
                     LNQAHDIAQELERNIRDEFGEDVEVDTHIEPLEPELPLGADAAPDRVDAIKTALSRFA
                     ADGAIHDIHSVRVRNTDAGEIVNFHCRAAPSMSVIKVHENVDAIERALRRAFPTVKRV
                     ISHAEPPDA"
     gene            1762..4086
                     /locus_tag="VGA15_02900"
     CDS             1762..4086
                     /locus_tag="VGA15_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9586676.1"
                     /translation="MVRAHAATGCVQSDSIKGLAQSIAKPAYHRLLIAEPALRRAVPT
                     LIIAFLITICLGAFVQVVDQNRQQRAAMKRDLAALADLLAERLDRIGSAREDRAATVD
                     HLQGLVPGLIPSWGIAAGRHVIVTGADQRILARVPIETSLGDPDRILDAISTAQLLAA
                     PAHQGAVTDMILPSGSVALAAQHVVKSLPGHVIIVQEKVDSLWRSDAALSVTLSATTG
                     FVVLILGFAFHWQSTRAREGDLINDAVRGRIDTALNRGRCGLWDWDLSRGRIFWSQSM
                     FTMLGLGTRSDLLTFGEVNALVKSDDIDLFAIADQLISSRINHIDQTFRMQHTDGHWI
                     WLRVRCELSQGEADAGLHLIGIAVDITEQKSLAEKTVEADLRLRDAIETIPEAFVLWD
                     AEDRLVLCNSHFQRLHKLPDSAVTPGTSYETVIEVGSMPEVRTRLHEAGTQPPGARTF
                     EAQLDNGSWLHISERRTKDGGYVSVGTDITRIKEHEQKLVDNDLRLRATVIDLKRSQT
                     ALEQQAIELADLAEKYSEEKNRAEEANQTKSKFLANMSHELRTPLNAIIGFSEIMESG
                     MFGTLGSEKYQEYCHDILTSGHYLLEVINDILDMSKIEAGRMKLDMESLDLSKTLAES
                     LRVVSGRADDKNLLLDADIEGTISVVADRRAIKQIIVNLLSNAVKFTPEGGKVIVRSR
                     VLSDSILLMIADTGIGIAAHSLARLGRPFEQVESQLTKTYHGSGLGLAIARSLTNLHG
                     GSMRLRSKLGAGTVVCVSLPRDAGKARAKMSVAA"
     gene            complement(4098..4991)
                     /locus_tag="VGA15_02905"
     CDS             complement(4098..4991)
                     /locus_tag="VGA15_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9586677.1"
                     /translation="MIDKLELLLALAKERHFGRAAEACGVTQPTMSTSLKQLEEILGV
                     MLVQRGSRFQGFTPEGERTLDWARRIVGDARAMRQEINGLKDKLSGEIRIAAIPTVLG
                     MVASLTTPFRARHPDVLFRIVSCTSADVLGLLENLEVDAGLTYIENEPIGKVRTIPLY
                     NESYRLLTAPDAMFGDRDSVTWKEVGQVPLCLLTADMQNRRIIDRALRSVGAEATPTL
                     TSNSLLVLYTHVKTGRWASVMPAKLAETLGLADTVRSIPIVDPVVTYSVGLVVPQRDP
                     MTPLIAALVHTVREVAPTLES"
     gene            5228..5704
                     /locus_tag="VGA15_02910"
     CDS             5228..5704
                     /locus_tag="VGA15_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX9586678.1"
                     /translation="MMPVYEPWNAARAVEIIAEHSHLEGATLVILHALQEAFGYVPEP
                     AVPMIASALNLSRAEIHGVFTFYHDFRREPAGRHVLKLCRAEACQAAGGDALVACAEA
                     RLGITIGNTTADERVTLEPIYCLGLCATAPSAMLDGRVVGRLNEARIDALIAEALR"
     gene            5701..>6668
                     /locus_tag="VGA15_02915"
     CDS             5701..>6668
                     /locus_tag="VGA15_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX9586679.1"
                     /translation="MTMRIFVSRDAGAVAVGADEVALVLGLAAGKRCIDIEIVRTGSR
                     GLYWLEPMIEVATSQGRIAFGPVTEFDASSVLDAMISGGAHPLRLGVADEIPWLKRQT
                     RLTFARCGVIDPRSLGDYRAHDGYKGLERALTLTSDAILADVTTSGLRGRGGAGFPTG
                     IKWKTVAQTGADRKYIVCNADEGDSGTFADRMIMEGDPFVVIEGMTIAGIAVGATKGY
                     IYIRSEYPHAVAAMNAAIAAATRAGYLGARIGGSSHSFDLEVRVGAGAYVCGEETSLL
                     ESLEGRRGIVRAKPPLPAHKGLFGKPTVINNVLSFAAIPFILAGGA"
BASE COUNT         1234 a         2151 c         2071 g         1212 t
ORIGIN      
        1 cctgcgtagg ctggtcctcg ttgtctggga cccgacggct cgcttggtcc gaccgagcac
       61 aggaagattt taaaatcgga acaattcgca acaatcgctg ccgcgatcga gtggcggctt
      121 tcgcacttga tcggcgcttt agtgttgcat gacgggctgc taaaccccgc ccatgattca
      181 tcccgatcac aacgccaaat cccgcgtcgc ggcgatttcg attttcgcca gcaccggcat
      241 ggcggcggcc aaattcgtgg tcggtattgc catcggcagc ctcgcgctga tttccgaagc
      301 gctgcactct tcggtcgacg tggtcgcgac cgtcatcacc tggctggtgg tgcgggtttc
      361 cgaccgcccc gcggaccacg aacatcatta cggccatggc aaatttgaga gcctgtcggc
      421 gctcggcgtc atcgcgatgc tttatgtgct ggcgggcggc attctggtcg aatcctacag
      481 ccgcctgcgc gagggtgcgc caccgccgac gatctcggcc atcccgttca tcgtcctgct
      541 gatcgacatc gccgtcaatt tctggcgcgc ccgggcgttg catcgaaccg cgcgcaacac
      601 caaaagccag gcgctggccg ccgatgcgct gcattttgca tccgacgtgc ttggttcggt
      661 cgccgtgatg atcggactgg gactatcggg actgggttat gcctggggtg acgccgtcgc
      721 cgcgatcggc gtggcggtcg tgatcgcgct gctgggcctg cggctggcgc gctccaccgt
      781 ggaaaccctg ctcgaccgcg cgcccgaagg cgcgtcggag aaagccatcg ccgcgatcag
      841 ggcggtgccc ggcgtggtcg atgtcgaacg cttaaggatg cggatggtcg gctcgacgca
      901 cttcattgac gccatcgtgc aggtgccgcg cacctatccg atcgaccgtg tcgaggaaat
      961 caagcgaagg gcgcaggctg cggtcagcaa agtgctcgac gatgccgatc tgacgttcac
     1021 cgcggtgccg gtggcgcgca acaacgagag catacgtgaa cgcatcatgg tgatcgcccg
     1081 caattccggg ctcgccatcc accatgtcac ggtgcatgac ctcggcggca agttgaccgt
     1141 cagcatcgac cttgaagtcg acgccgatat ggcgctcaac caggcccacg acatcgccca
     1201 agagctggag cgcaacatcc gcgacgaatt cggtgaggac gtcgaggtcg acacccacat
     1261 cgagccgctg gaaccggaat tgccgctcgg cgccgacgcc gcgcccgacc gcgtcgatgc
     1321 catcaagacc gctttgtcac gctttgccgc cgacggcgcg atccatgaca tccacagcgt
     1381 gcgggtgcgc aataccgacg cgggcgagat cgtgaacttc cattgtcgcg ccgcgccgtc
     1441 gatgagcgtg atcaaggttc acgagaacgt cgatgcgatc gaacgcgcgt tgcgccgcgc
     1501 gttcccgacc gtgaaacgcg tgatcagcca cgccgagccg ccggacgcct agtaaaatct
     1561 gaaatccccg gcgcgttccc ctttcggact cactttgcac ctcatttcgg agttccaatt
     1621 ctgcagtgga caagatttat ttgcattaac gattcgttga ctctcgacag cccgccaaaa
     1681 ctggattcaa atcgctttga ttcggaagtg actggggttc tttcgaagaa ggtgcaaaga
     1741 gatctccgag gggcctacgg catggtgcgc gcgcacgcgg cgaccgggtg cgttcaatcc
     1801 gattcgatca agggattggc gcagtcgatc gctaaaccgg cctatcatcg gctgttgata
     1861 gcggagcccg cacttcgccg cgcagtgcca acgctgatca ttgcgttcct gatcaccatc
     1921 tgtcttggcg ccttcgtgca ggtggtcgat cagaaccggc aacagcgcgc ggccatgaag
     1981 cgggatttag cggcgctggc cgatttgttg gccgagcgcc ttgaccgcat tggttcggcc
     2041 cgagaggatc gcgcagcgac cgtcgaccac ctgcagggcc tggtgcccgg cctcattccg
     2101 tcatggggca tcgccgccgg ccggcacgtc atcgtcaccg gcgcggacca gcgcattctt
     2161 gcccgcgttc ccattgaaac aagcctcggc gaccccgacc gtattctcga cgccatcagc
     2221 acggcgcaat tgttggccgc gcctgctcac cagggtgccg tcaccgacat gatcctgccg
     2281 agcggcagcg tcgcgctggc ggctcagcac gtcgtcaaat cgctgcccgg tcacgtcatc
     2341 atcgtccagg aaaaggtcga ctcgctgtgg cggtcggatg cggccctgtc ggtgacgctg
     2401 tccgccacca ccggctttgt cgtgctgatc ctggggttcg cattccactg gcaatcgacc
     2461 cgtgcgcgcg aaggcgatct catcaatgac gcggtgcgcg gccggatcga taccgcgctc
     2521 aatcgcgggc gctgcgggtt gtgggactgg gacctgtcgc gcggcagaat cttctggtcg
     2581 cagtcgatgt tcacgatgct cggcctcggc acccgcagcg accttttgac cttcggcgag
     2641 gtcaacgcgc tggtgaaatc cgatgacatc gatctgttcg cgatcgccga tcagcttatc
     2701 tcctcgagaa taaaccatat cgaccagacc ttccgcatgc agcacaccga cggccactgg
     2761 atctggctgc gcgtccgctg tgagctcagc cagggcgagg ccgatgccgg cctgcatctg
     2821 atcggcattg ccgtcgacat caccgagcaa aaaagcctcg ccgagaaaac cgtggaggcc
     2881 gacctgcggc tgcgcgatgc gatcgagacc atcccggaag ccttcgtgct gtgggacgcg
     2941 gaagaccgcc tcgtgctctg caactcccac ttccagcggc tccacaagct gccggactcg
     3001 gcggtgacgc cgggcacctc ctatgaaacc gtgatcgaag tcggcagcat gccggaggtc
     3061 cgcacccggc tgcatgaagc cggcacccag ccgcccggcg cgcgcacctt cgaggcgcaa
     3121 ctcgacaacg gcagctggct gcatatcagc gagcggcgca ccaaggacgg cggctatgtc
     3181 tcggtgggca ccgacattac ccgcatcaag gaacatgaac agaaactggt cgacaacgac
     3241 cttcgcctgc gcgccaccgt gatcgatctg aaacgttcgc agaccgcgct cgagcagcaa
     3301 gccatcgagc tcgccgacct cgccgaaaaa tattccgaag agaagaaccg cgccgaggaa
     3361 gccaaccaga ccaagtcaaa attcctcgcc aacatgagtc acgaattgcg cacgccgctc
     3421 aatgccatca tcggattttc cgagatcatg gaaagcggca tgttcggaac gctgggatcg
     3481 gagaaatacc aggaatactg ccacgatatc ctcaccagcg gtcactattt gctcgaagtg
     3541 atcaacgaca tcctggatat gtcgaagatc gaggccggcc gcatgaagct cgacatggaa
     3601 tcgctcgatc tgtcgaagac gctggcggaa tcgctgcgag tggtgtcggg gcgcgccgac
     3661 gacaagaatc tgctgctcga tgcggacatc gagggaacca tttccgtggt ggcggaccgt
     3721 cgcgcgatca agcagatcat cgtcaacctg ctgtccaacg cggtcaagtt cacgcccgag
     3781 ggtggcaagg tgatcgtccg cagccgcgtg ctcagcgatt ccatcctgct gatgattgcc
     3841 gataccggca tcggtatcgc ggcgcattcg ctggcgcgcc tcggccgacc gttcgagcag
     3901 gtcgagagcc agctcaccaa gacctatcac ggctcggggc ttggcctcgc catcgcaagg
     3961 tcgctcacca acctgcatgg cggatcgatg cggctgcgct cgaaactcgg cgccggcacc
     4021 gtggtctgcg tgtcgctgcc gcgcgacgcg ggcaaggcca gagcgaagat gtccgtggcg
     4081 gcttaattgc ctggccttca ggattccagc gtcggggcga cttcgcggac ggtgtggaca
     4141 agggccgcaa tcagcggcgt catcgggtcg cgctgcggaa ccaccagtcc gacactatag
     4201 gtgacgaccg gatcgacaat cggaatgctc cggacggtgt ccgccaagcc aagcgtttcc
     4261 gcgagcttcg ccggcatcac gctggcccag cgcccggtct tcacatgggt gtaaagaacc
     4321 agcaacgagt tcgaggtcag cgtcggcgtc gcctcggcgc cgaccgagcg cagcgcgcgg
     4381 tcgataatgc gccggttctg catgtccgcc gtcagcaggc acagcggcac ctgcccgact
     4441 tccttccagg tgacggaatc gcgatcaccg aacatggcgt cgggcgcggt cagcaggcga
     4501 tagctctcgt tatagagcgg tatggtgcga accttgccga tcggttcgtt ctcgatgtag
     4561 gtcaacccgg catcgacctc cagattttcc agcaggccca acacgtcggc cgacgtgcac
     4621 gacacgatgc ggaacagcac atccggatgg cgggcgcgaa acggcgtcgt cagcgacgcc
     4681 accatgccca gaaccgtcgg gatcgccgcg atcctgatct cgccggatag cttgtccttg
     4741 aggccgttga tttcctgccg catcgcgcgg gcgtcgccga caatgcgccg cgcccagtcg
     4801 agcgtgcgtt cgccttcggg cgtaaagccc tggaaccgcg agccgcgctg caccagcatc
     4861 acgccaagga tttcctcgag ctgcttgagg ctggtcgaca tcgtcggctg ggtgacgccg
     4921 caggcttccg cggcgcgtcc gaagtgacgt tcttttgcca gcgcgagcag aagttcgagc
     4981 ttgtcgatca agagagcctc accgttccag cgctacgaat atcacgatct caaaccacaa
     5041 atatgcaaaa agtcgcgcca aatgagcacc gccgccacgc tcacaccggc tcattccatt
     5101 ttcctatggc ctgatcttat aaccctatcg atcgcatttc cgtatcgcag catgagacag
     5161 ctttattgat ttcttgatca atcccaattc tcattggccg ccaataggta gctgacagag
     5221 aacgtggatg atgccggttt acgaaccatg gaatgccgcg cgcgccgttg aaatcatcgc
     5281 ggagcacagc caccttgaag gcgcgacgct ggtgatcctg cacgccctgc aggaggcgtt
     5341 cggctacgtg ccggagccgg ctgttcccat gatcgcatcg gcgttgaacc tgtcccgcgc
     5401 cgagatccac ggcgtcttca ccttctacca tgatttccgg cgcgagcccg ccgggaggca
     5461 cgtactgaag ctgtgccgcg ccgaagcctg tcaggccgcg ggcggcgatg cgctggtggc
     5521 gtgcgcggaa gcgcggctcg gcatcaccat tggaaacacc acggcagacg agcgggtgac
     5581 gctggagccg atctattgcc tcggtctgtg cgccaccgcg ccatcggcga tgctggatgg
     5641 ccgcgtggtc gggcggctga acgaagcgcg catcgatgcg ctgatcgcgg aggcgctgcg
     5701 atgacgatgc ggattttcgt atcccgcgac gcaggcgctg tcgcggtcgg agccgatgag
     5761 gtcgcattgg tgctggggct ggcggccggc aagcgctgca ttgacatcga gatcgtcaga
     5821 accggctcgc gggggctcta ttggctggag ccgatgatcg aggtcgcgac atctcaaggg
     5881 cgcatcgcgt tcggtccggt gaccgagttt gacgccagtt ccgtgctcga tgcgatgata
     5941 tcgggcggcg cgcaccccct gcggcttggc gtcgcggacg agatcccctg gctgaagcgg
     6001 cagacccgcc tgaccttcgc ccgctgtggc gtgatcgatc cgcgttccct gggggattat
     6061 cgcgcccatg atggctacaa gggcctggag cgcgcgctga cgttgacgtc ggacgccatc
     6121 ctcgccgatg tcacgacttc cggattgcgc ggccgtggcg gggccggctt cccgaccggg
     6181 atcaaatgga aaaccgtggc gcagaccggc gccgaccgga aatacatcgt ctgcaatgcc
     6241 gacgaaggcg acagcggcac cttcgccgac cgcatgatca tggaaggcga tccgttcgtc
     6301 gtgatcgagg gcatgacgat tgcgggcatc gccgtcggcg ccaccaaggg ctacatctac
     6361 atccgctcgg aatatccgca cgccgtcgcg gcgatgaatg cggcgatagc ggcggcgacg
     6421 cgcgccggct atctcggcgc gcgcatcggc ggctccagcc acagcttcga tcttgaagtc
     6481 cgcgtcggcg ccggcgccta tgtctgcggc gaggaaacct cgctgctgga aagcctcgaa
     6541 ggccgccgcg gcatcgtccg cgccaagccg ccgctgccgg cgcataaggg cctgttcggc
     6601 aagcccaccg tgatcaacaa cgtgctgtca ttcgcggcga tccccttcat cctcgccggc
     6661 ggcgccaa
//
LOCUS       DASWGM010000094        16063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_560463,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000094 DASWGM010000000
VERSION     DASWGM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16063
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560463"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGA15_02920"
     CDS             <1..273
                     /locus_tag="VGA15_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9586680.1"
                     /translation="ALHSIEARFCRWLLQSADRAANDTVALTQEFLAEMLGVRRTSVT
                     EVASKVQSAGAITYSRGVIKILDRPALLRMSCECYETLVDQSAQLG"
     gene            638..2923
                     /locus_tag="VGA15_02925"
     CDS             638..2923
                     /locus_tag="VGA15_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9586681.1"
                     /translation="MTTLRRIAFIGNSLPRRCGIATFTTDLQQAISTSQPEIEASIMA
                     MTDHGQAYDYPAAVALQIKDDNIDEYVRAADFLNTGRFDAVCLQHEFGIFGGEAGVHI
                     LVLLSRLTMPVVTTLHTVLAEPTTAQRAVMQRIVEASSKVVVMAKKGRELLHTVYQVP
                     DDKIEVIPHGIPDVAFVAPDAAKARLGFAGRSVILTFGLLSPNKGIEVMIDAMPSILK
                     RRADAVYVVLGATHPNLVRDQGEAYRESLMSRVRELGVEDHVVFLDHFVDQTTLLEFI
                     SMCDVYVTPYLNEAQMTSGTLAYSFGLGKPVVSTPYWHACELLADGRGVLIPFGDAAA
                     TGREIAELLTDTPRRQAMCKRAYAVSRSMTWQRIAERYLSVFESARQVQWLKALARPD
                     KALPETRSPAAPDMQIGHFLSMCDDTGLFQHAVHSVPDRSHGYCVDDNARALLLACAL
                     NNPGERPLPEFLTARFAAFVQHAWNPDTKRFRNFMGFNRTWLEDSGSEDSHGRTLWAL
                     GECARSDASPSRRRWAAVLFAQALPTAESFTSPRAWGFTLLGLDSYCAVVANDLHARD
                     VRLVLADRLMRILASVETPDWVWFETGLSYDNARLPQALLLTGMATKTPSHVDAGLKS
                     LHWLMRQQTAPAGHFRPVGSAGFGEQRQPPRAFDQQPVEATATIAACLAAWRADGDAE
                     WKAVATRVFAWFLGSNDLSVALADPETGSCCDGLHPDRANENLGGESVLSYLLGLAEI
                     RQLARSTAGLTRPQPLRALRA"
     gene            2987..4285
                     /locus_tag="VGA15_02930"
     CDS             2987..4285
                     /locus_tag="VGA15_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HEX9586682.1"
                     /translation="MLQTTFLNRQALYLRPDPARVIVRPFKPATEPRDLNPTDKTRAN
                     HIVDRVLALDSEAVAHQLADVLENFQGRHRNLLETFEARADVMEEVLAAHGTFSKVQR
                     QLIGAYFLNEYSFEASALFNPSIVRHPDQSGAPKGGLRFILSLRAVGEGHVSSLTFRA
                     GTIAADGSLTVDPTARLASSPRISRRVSAPDGDHIELTFNPEEELSERVIFPVTESQS
                     NGIEDARFVEFSDEGRKTYYATYTAYSGRAIRSELIATHDFMSFWLAPLRGTAAQNKG
                     MALFPRKIVGKYAMIARQDNENLYLIYSDDLHTWDGGQAILKPEFPWEFVQIGNCGSP
                     IELDEGWLLLTHGVGPVRKYSIGAALLDKNDPSKVLARSREPLLRPEPSEREGYVPNV
                     VYTCGAMRHNDQIILPYAVSDSYSNFATIRIPALLQTMKR"
     gene            4347..4589
                     /locus_tag="VGA15_02935"
     CDS             4347..4589
                     /locus_tag="VGA15_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586683.1"
                     /translation="MTRETSYFVQAFNAGKGGNLKPDAPIACKTANGALRTAERLALS
                     KLGVVAFSSTGDPEMGDYDDEPTVFFRNGRLPAAFD"
     gene            complement(5267..5542)
                     /locus_tag="VGA15_02940"
     CDS             complement(5267..5542)
                     /locus_tag="VGA15_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586684.1"
                     /translation="MKVFRIAAVMALLTAPAYAQTPNINLIPELQSKSPEEKEQEAAQ
                     QKAYKESLKKIPDAKTSSDPWGNVRSVDTPKASAPAKPRTKTGSTAN"
     gene            5883..6347
                     /locus_tag="VGA15_02945"
     CDS             5883..6347
                     /locus_tag="VGA15_02945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9586685.1"
                     /translation="MQYEPQTEDFDIEEARRVLGDVFAPWVQDLGLSVGNIECAPPPG
                     APADWQPGAILRMAFSERLCRHGGIVCGQALMALADTAMVIAILAANRAYRPMTTVDQ
                     TTHFMRAVASSDVLADARVVRLGRTMSFGRVTLSSATDNKPVAMVSSAFAML"
     gene            complement(6355..7866)
                     /locus_tag="VGA15_02950"
     CDS             complement(6355..7866)
                     /locus_tag="VGA15_02950"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9586686.1"
                     /translation="MFRPVLRHALLAAGILLGSHAAFAENRVALVIGQSNYHSVVALP
                     NPANDARAMSRLLGDAGFDVTSAADLSQNEMREKVSDFAVKVAAKGPDTVALVFYAGH
                     GLQIDGENFLVPVDVDPKREADIPLQAIRLNDVLNTLTSVPSKTRILLLDACRNNPFP
                     AINGTAGRGLAIVDAKTGAPGTFMSFSTSPGAEAEDGSGADSPYTTALLSAAKEPGLS
                     IEDTFKRVRVSVNKVTEGRQTPWDSSSLTDDFRFFSGPSAPAPKPVAVQRTVDQWRRE
                     LQGKPVEAANELIVADGTEEAYEAFVELFAQTPFGPQAREWLDRHRRMVAWNNAVIIN
                     TAAAYRAFLVQYPDSDLTVTARKLEDRLRYRPNIAGSLALAVGGAPNGSGASAPASPG
                     GAPLPTNASLAAPTCPCGGTPALPLRKVDLPPKKHADPEPPKRASVRPPPRGPREPPP
                     DDDVVVYRRSPPPDYYEPSPVPSIGIGIGMGLGRIGGGGGDRGGGMVGRRGGY"
     gene            complement(7941..8171)
                     /locus_tag="VGA15_02955"
     CDS             complement(7941..8171)
                     /locus_tag="VGA15_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586687.1"
                     /translation="MKTLTVAAVTLALIAIPAHAQMQGKRHNNDEKKPDQKENRVDEK
                     AYKAALERIPDSKEKYDPWGIARPADTAKKPK"
     gene            8354..8761
                     /locus_tag="VGA15_02960"
     CDS             8354..8761
                     /locus_tag="VGA15_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586688.1"
                     /translation="MLRQIVHSALMVGAVIALAGCGIADSRSPVPEFMRAGVSEPPPP
                     EPPPDVGRLIHEQLDSVFTAASYPRQVRVSPPHREPYGSGWSACVRAELTSVMGKPLG
                     TETYRVTISGGTIVDRRRAETDDNCVSETYQPI"
     gene            9135..12476
                     /locus_tag="VGA15_02965"
     CDS             9135..12476
                     /locus_tag="VGA15_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9586689.1"
                     /translation="MDVGTWLSGLGLSQYEAVFRESEIDVDVLTELTDQHLKDLGVAL
                     GHRLRMLRAIRELAGDAPVKASAAKPQDAADRRQLTVMFCDLVDSTALTARLDPEDMG
                     DLLRAFQRAVAAAVTRFDGHVAKWTGDGASVYFGYPRAHEDDAERATRASIALVDAVG
                     KLRRERGIALEVRIGISTGLVVVGELIGEGEARERGVVGDTPNLASRLQALAEPDTVV
                     VSESTRRLLGRNFELQPLGPQELKGFKSPVPAWSVLRVSENVNRFEASRSGAMTPFVG
                     REQEIALLINRWRRATKGEGQVALLSGEAGIGKSRILAVLRERIGDERHLAFRYQCSP
                     HHGNDAFYPVIGQIWRSAGLVSGEPAAARLDKLEMMILRAGLDIGEIVPYLASLLAIP
                     TEGRYPALEMSPSEVKERTIAALIAMVVGIAKSVPVVMLLEDAHWIDPTSLDLTGQLV
                     ERVQNLAVLIVLTARPEFEAPWIGRPNFTALALNRFERSQVVTMIDRIMSSRALPTEV
                     LDQIIAKTDGVPLFVEELAKSVLESGLLREENGAYVLAAALTPLAIPSTLHDSLTARL
                     DRLSPIKEIAQIGATIGREFSYDLLEAVSPVRGQALHNALQQLMETELIYGRGTPPKA
                     SYIFKHALVQDTAYVSLLRGRRQRIHADIALVLKERSAGEECPPAIIAHHFTEAGLAE
                     QAAPYWLAAAELALSQSAPVEAEHHASAGLALIPRVTEGPERDALELGLLVARANAMI
                     PLKSISAPETFAALADAKQLLDRGVGTDLQRVSILYGLCSASTLTARLKQALDFAHQI
                     IEVAERQDDPTYRVVGYRQLGTLQFYAGKNREALQSLQKGHQYCDPRRQRALGYRFNW
                     DQGVALLSFEVLVRLSLGLLDSAAQLSERVRIELLSHGHAATVASATFASQTWPKLVL
                     GDLEALERESAELAAYCAEKRVEQIRLLASYHYAYACAMRDPVERNIAVLLAALDALR
                     GSGGNAGNSIIKCNLAEALLTAGDLAGAEAVLEEGFAFVEQSGEQYWLAGLHRLCGHV
                     ALKRPEPDRLRAEACFKKAIEVARSQEARLLELRAAVDLAQLWRDTGSDGDARAMLEP
                     ILAAIEGGETTRDVRNARAALAGLV"
     gene            12920..14164
                     /locus_tag="VGA15_02970"
     CDS             12920..14164
                     /locus_tag="VGA15_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586690.1"
                     /translation="MSPNADGNNPSDPVGSFARTTAQGLGSLWGMMLEHLSEKHAKAA
                     AAWSENQEGGVGRTPDGILVTSKELVMQVLLNADGNYTIEGYQARMNQSIGLIYLGMD
                     WGPEYVKQSTKSNEAIGRVTRKEAFDFALKETRAALAALADPADFDIVKVSDTVFAGI
                     CNHWWDIPDGEFVQAGGFRLSNLAGPARCPGDYTPPSGYIVHPDPDLLLAHIGQRTGQ
                     ILREAVGKFVASRRDPKNPPVGAITKALFEEFPNPEDNDLLARTIVGVMMGALPTING
                     NLITIVKTWQKTATLFSLQAKLKKSSQPDEFAKAHEVIEQPMQETMQMTPTPDALWRM
                     AVKDHTLGTTHPVQVRAGDKIYLSVVRATQEDLRNGITDVRPLFGGNRSLTPHPTHAC
                     PGFEMGFGILLGIVYGCVEWQP"
     gene            complement(14342..14608)
                     /locus_tag="VGA15_02975"
     CDS             complement(14342..14608)
                     /locus_tag="VGA15_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586691.1"
                     /translation="MIRYLAIIGTLAVFAGAPAVAQQSTAPGQTTQDKDSAKGAKGAA
                     ENARGERGEMQDKGTAKGTKPGLGVTTGSGSGAGSRHTGNDSPR"
     gene            complement(14853..15125)
                     /locus_tag="VGA15_02980"
                     /pseudo
     CDS             complement(14853..15125)
                     /locus_tag="VGA15_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            15137..15976
                     /locus_tag="VGA15_02985"
     CDS             15137..15976
                     /locus_tag="VGA15_02985"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX9586692.1"
                     /translation="MVGLPAENREGLSTTIVQQAGSILLEVEKRNVRHFEVETPYLAA
                     VVKGTQFRVSVNAASTRIDVIRGQVEVADFRSGQVAQVMPGQVATAFARGKPGLSLSG
                     TGTFSPIEQGRPRASTIQQITVPKSGLSAPRHAANGQFNRALGHGDAGGARHGVTRIL
                     ASLGEVRLNFHKVTHGLARGAISTPGSARSAANKDTVWTSREAAATLGGGNGSSQGNS
                     GDGSSSTANVATGVSAATIAAVTGPGNSNGDGNGNGNGNANGNGNGNGNGNGHGLAKG
                     HNK"
BASE COUNT         3013 a         5140 c         4942 g         2968 t
ORIGIN      
        1 gcgctgcact cgatcgaggc gcgattctgc cgctggctgc tgcagagcgc ggaccgggcc
       61 gctaacgaca cggtcgcgct gacgcaggaa tttctcgccg aaatgctcgg cgtgcggcgc
      121 acgtcggtga ccgaagtcgc gagcaaggtg caaagcgccg gcgcgatcac ctattcgcgc
      181 ggcgtgatca agatcctgga ccggcccgcg ctgctgcgga tgtcgtgcga gtgctacgaa
      241 acgctggtgg accaatcggc ccagctgggc tgacggcccg ggtggcgtcc atttcccgcg
      301 gttttgcgcg gcgatgagcc cttcgatcac ccgcggcggc ccccttctca tcattttgcg
      361 gttttcgggc aggatatgag cgcgagggtc tttagcatcg cacgcaggtt gctatcgggt
      421 gccggagtag ccatctgagc aagtggtacg atcggatcgc ttcaacagcg catgtcccga
      481 atcgcgacac ttagccacgc caaagcttac agacggtatc gaccaacggc atatcgcttc
      541 ggtatgcgcg tcacgggtca ggacaaccta tgcccatgga acatgcggcc gagcaggtcg
      601 tgcgcaagca atcaaacggg attgtcgagg cgcagacgtg acgacgctcc gccgcatcgc
      661 ctttatcggc aactcgcttc cccgccgttg cgggatcgcc accttcacca ccgacctgca
      721 gcaggcgata tcaacgtcac agccggaaat cgaagccagc atcatggcga tgaccgacca
      781 cggtcaagcc tatgactatc ctgcagcggt cgcgttgcag atcaaggacg acaatatcga
      841 cgagtacgtg cgcgcggccg atttcctgaa caccggccgg ttcgacgccg tctgtctgca
      901 gcacgaattc ggaattttcg gcggcgaagc cggcgtccac atcctggtgc tgctgtcgcg
      961 cctcaccatg cccgtggtga cgacgctgca taccgtgctg gccgagccca cgaccgcgca
     1021 acgcgcggtg atgcagcgca tcgtcgaggc gtcgtcgaag gtcgtcgtga tggccaaaaa
     1081 gggccgcgaa cttctgcata ccgtctatca ggtgccggat gacaagatcg aggtcattcc
     1141 ccacggcatt cccgacgttg cgttcgtcgc gccggacgcg gccaaggcca ggctcggatt
     1201 tgccggacgg tcggtcattc tgactttcgg cctgctgtct cccaacaagg gcatcgaggt
     1261 catgatcgac gccatgccgt cgattctgaa acgccgagcc gacgcggtat atgtcgtgct
     1321 cggcgcgacc catcccaatc tggttcgcga ccagggcgag gcctatcgag agagcctgat
     1381 gtcgcgcgtg cgcgagctcg gcgtcgagga ccacgtggtg ttcctcgacc atttcgtcga
     1441 tcagaccacg ctgctcgaat tcatttcgat gtgcgacgtc tacgtcacgc cctatctcaa
     1501 cgaggcgcag atgacgtcgg gcacgctcgc ctacagtttc gggcttggaa agccggtggt
     1561 ctcgacgccg tactggcacg cctgcgaatt gctggccgac ggacgcggcg ttctgatccc
     1621 gttcggcgac gccgcggcca cggggcgaga aatcgccgaa ctgctcaccg acacccctcg
     1681 ccgccaggcg atgtgcaagc gcgcctacgc ggtcagccga tcgatgacat ggcagcgcat
     1741 cgccgaacgt tatttgtccg tattcgagag cgcgcggcag gttcaatggc tcaaggccct
     1801 cgcgcggccc gacaaggcct tgcctgaaac gcgcagccct gctgcgcccg acatgcaaat
     1861 cggccacttc ctctcgatgt gcgacgatac cggcctgttc cagcacgccg tgcattcggt
     1921 gcccgatcgc agccacggct attgcgtcga tgacaatgcc cgcgcgctgc tgttggcctg
     1981 cgcgctcaac aatccgggcg aacggccttt gccggagttt ctgaccgccc gctttgccgc
     2041 tttcgtgcag catgcctgga atcccgacac caaacgcttt cgcaatttca tgggcttcaa
     2101 tcgaacctgg ctcgaagact ccggctccga agacagccac gggcgaacgc tgtgggcgct
     2161 cggtgagtgc gcgcgcagcg atgcaagccc gtcgcggcgc cgatgggccg ccgtcctgtt
     2221 cgcgcaagcg ctccccaccg cggaaagctt tacctcgccg cgcgcctggg gttttacgct
     2281 gctcggcctg gactcctatt gcgcggtcgt cgccaatgac ctgcacgccc gcgatgtccg
     2341 gctggttctc gccgaccggc tgatgcgcat tctcgcctcc gtcgagacgc cggactgggt
     2401 gtggttcgag acaggacttt cctacgacaa tgcgcgcctg ccgcaggccc tgttgctgac
     2461 gggcatggcg accaagacgc ccagccatgt cgacgccgga ttaaaatccc tgcattggct
     2521 gatgaggcag caaacggcgc cggccggcca tttccgcccc gtcggcagcg ccggcttcgg
     2581 cgagcagcgg caaccgccgc gcgccttcga tcagcagcct gtggaagcca ccgcgacgat
     2641 cgccgcctgt ctcgcggcat ggcgggccga tggcgatgcc gagtggaagg ccgtcgccac
     2701 gcgcgtcttc gcctggttcc tcggcagcaa tgatctgtcg gtcgcactgg ccgaccccga
     2761 gaccggcagt tgctgcgacg gcctgcatcc cgaccgtgcc aatgaaaatc tcggtggcga
     2821 gtcggtcttg tcctatctgc tcggtcttgc ggaaattcgt cagcttgcgc gttccacagc
     2881 cgggctgaca cgaccccaac cgctgcgcgc gttgcgcgcc tgaaatcctg gtatcgttct
     2941 ctagagtaac gtccgaacag tcaacctcca acgcgccgag ggcaccttgc tgcaaaccac
     3001 attcctaaac aggcaggcgc tttaccttcg gcccgatccg gcgcgcgtca ttgtgcgccc
     3061 gttcaagccg gcgacggagc cgcgcgatct caacccgacc gacaagaccc gcgccaatca
     3121 tatcgtcgac cgggtgctcg cgctcgattc cgaggccgtt gcccaccagc tcgccgatgt
     3181 cctggagaat ttccagggcc ggcatcgcaa cctgctggag acgttcgagg cgcgcgccga
     3241 cgtcatggaa gaggtgctgg cggcgcacgg caccttctcc aaagtgcagc gccagttgat
     3301 cggcgcctat tttctcaacg aatattcgtt cgaagcctcg gccctgttca acccctcgat
     3361 cgtgcggcac ccggaccagt ccggcgcgcc gaaaggcggc ttgcgtttca ttctcagtct
     3421 gcgtgccgtc ggcgaagggc atgtgtcgtc cttgacgttt cgcgccggca ccatcgccgc
     3481 cgacggcagc ctgacggttg acccgaccgc tcgcctcgcc tcgagtcccc ggatcagccg
     3541 ccgcgtatcc gcaccggacg gcgaccatat tgagctgacc ttcaatccgg aagaagaact
     3601 cagcgagcgc gtgatctttc cggtcaccga atcccaatcg aacggcatcg aggatgcgcg
     3661 ctttgtcgag ttcagcgatg agggccgcaa aacctattac gcgacctaca ccgcctatag
     3721 cggccgggcg atccgctccg aattgatcgc gacccacgat ttcatgtcgt tctggctggc
     3781 gccgttgcgg ggcactgcgg cgcagaacaa gggcatggcg ctgttcccgc gcaagatcgt
     3841 cggcaaatac gccatgatcg cgcggcagga taacgagaac ctgtatctga tctattctga
     3901 cgacctccac acctgggacg gcggtcaggc gatcctgaaa ccggaatttc cctgggagtt
     3961 cgtccagatc ggcaattgcg gatcgccgat cgaactcgat gaaggctggc tgctgctgac
     4021 gcacggcgtc ggcccggtcc gcaagtattc gatcggcgcg gcgctgctag acaagaacga
     4081 tccctccaag gtgctggcgc gttcgcgcga gccactgctg cggcccgaac cgtcggagcg
     4141 cgagggctac gttcccaacg tcgtctacac ctgcggggcg atgcggcaca acgaccagat
     4201 cattttgccc tacgcggtgt cggacagtta ttccaatttc gcgaccatcc ggattccggc
     4261 gctgctgcag acgatgaagc gttgatcgcc gcgctcaaaa accgtacccg ccgaccgcgc
     4321 ttgttgacag ctcaaaggag cccctcatga cccgcgagac cagctacttc gtgcaggctt
     4381 tcaacgccgg caagggcggc aacctgaagc ccgatgcgcc gatcgcgtgc aagacggcaa
     4441 atggcgcgct gcgcaccgcg gaacggctgg ccctgagcaa actcggtgtc gtcgcctttt
     4501 catccaccgg cgaccctgaa atgggcgact atgacgacga gcccaccgtc ttcttccgca
     4561 atggccggct tccggcggcc ttcgattgag atctcgaatg cggtgcctat aacggcgcaa
     4621 cgccgccgag ctgaaataat gctcggcggc gaggcactag aactggaaat cgcaatcccc
     4681 cagcgcggtt atcatcgcac aggacacgcg aaagtcaccc cgtaacacta cgggggccga
     4741 atcgtcgtta tgagctggcg gcattcacgc caaaagagca atcatgatcc gcgcggtttc
     4801 ccctaaccca caggacggga tggcgtctgc cctttgtcat gcttacgccc gcccgagcga
     4861 cctgtttcca caggaattcg ttttcggacg ccattcgaga gccaacagaa atagacggga
     4921 gatagacgtg tcgcgccgct tcgagcgaaa aggggatcgt cgatgaaaac gcctgaacca
     4981 aaagaagtct ggacagaaaa aatctgctcg gcctgtaacg gcaccggatt tccgccgatc
     5041 aagcaaccga cacagccggg ccgcaaaatt tatccggcgc cctgcgagca atgttccggc
     5101 aagggccgaa taagggtcgc ctcgccgctc ccataacctt cgccgtaccg cgcgaccggg
     5161 tggccctgcc tcccgcggcc ttggttcgcg tttcaatcaa tccgccgcgt tggatttgac
     5221 cggggtcggt cacgcgcccg gcgaccgccg ctcgtccctt tcgggcttag ttggcggtgc
     5281 tgccggtctt ggtgcgcggc ttggcaggcg ccgatgcctt gggcgtgtcg acgctgcgca
     5341 cattgcccca gggatcggaa gaggtcttgg cgtccggtat cttcttcagc gattccttgt
     5401 aggccttttg ttgtgcggcc tcctgctcct tttcctcagg gcttttggat tgcagctccg
     5461 ggatcagatt gatgttgggg gtttgcgcgt aagccggcgc tgtcagcaat gccatcaccg
     5521 ccgctatgcg gaaaactttc atggaacgct ccgtggattt cccgtcaaca taccaccgca
     5581 ccgaccgcga cgccaagcac agcacttcgt gccccaaaaa tggtccgact gcaggatttt
     5641 ggcgattgca gccaatcatc ccagatcgga ccgcatttgt tgcagccatt gagcaccgga
     5701 cccatggcga ccatgctgaa aatcaatgct cgcctgaact tcgtcgacca ctcccctttc
     5761 ccatcatgca gcctcaagtc aggcattttc aagccgcgca acagccctgt ccctttccgc
     5821 cgaatgccct ttagaatgcg tgagaatacc cgcagccgcg tcgaacgagg aaccgcagtc
     5881 tcatgcaata tgaaccacaa accgaggatt ttgatatcga ggaagccagg cgcgttcttg
     5941 gcgacgtgtt cgccccgtgg gtgcaggatc tcggtctatc ggtcggcaat atcgaatgcg
     6001 ccccgccgcc cggcgcgcct gccgactggc agccgggagc gatcctgcgc atggcgttct
     6061 ccgagcgcct gtgccggcat ggcggcatcg tctgcggcca ggcgctgatg gcgctggccg
     6121 ataccgccat ggtgatcgcc atcctggccg ccaaccgcgc ataccggccg atgacgacgg
     6181 tcgatcagac cacgcatttc atgcgcgccg tcgcgtcctc ggacgtactg gccgatgcgc
     6241 gggtggtgcg cctcgggcgc accatgagct ttggccgcgt caccctgtcg agcgccaccg
     6301 acaacaagcc ggtggcgatg gtctcaagcg cctttgcgat gctgtgagcc gcaatcagta
     6361 tccgccccgt cggcccacca tgccgccgcc gcgatcgcct ccacctccgc cgatccgtcc
     6421 caaccccatc cctattccga tcccgatcga gggtaccggc gacggttcgt aataatcggg
     6481 cggcggcgag cggcggtaga ccacgacatc gtcatcgggt ggtggttcac gcggcccgcg
     6541 cggcggcggc cgcacgctgg cgcgcttggg cggctccgga tcggcgtgct tcttgggcgg
     6601 cagatcgact ttcctcagcg gaagggccgg cgtgccaccg caggggcatg tcggggcggc
     6661 cagcgaggca ttggtcggca gaggcgctcc gcccggcgaa gccggcgccg aggcgcctga
     6721 cccgttcggc gcgccgccga cggccaaggc caggctgccc gcaatattcg gacggtagcg
     6781 caggcgatcc tcgagcttgc gcgcggtcac ggtgagatcg ctatcggggt attgcacaag
     6841 gaaggcgcga tacgccgccg ccgtgttgat aatcaccgca ttgttccacg ccaccattcg
     6901 gcggtgacga tcgagccatt cgcgggcctg cgggccgaac ggggtttgcg cgaataactc
     6961 gacaaacgcc tcataggctt cttcggtgcc gtcggccaca atcagttcat tggcggcctc
     7021 gaccggcttg ccctgcagtt cacgacgcca ttgatcgacg gtgcgctgca cagccaccgg
     7081 cttcggtgcc ggcgcgcttg gtccggaaaa aaatctgaag tcgtcggtca gcgaggagct
     7141 gtcccagggg gtttgccgcc cctcggtcac cttgttgacg gaaacccgca cgcgcttgaa
     7201 ggtgtcttcg atcgaaagcc ccggctcctt ggcggccgaa agcagtgctg tcgtgtaggg
     7261 actgtcggcg ccgctgccgt cctcggcttc ggcacccggc gacgtcgaaa acgacatgaa
     7321 cgtgccggga gcgccggtct tggcgtcgac aatggccagc ccgcgcccgg cggttccgtt
     7381 gatcgcgggg aacgggttat tgcggcacgc atcgagcagc aggatgcggg tcttgctcgg
     7441 caccgatgtc agcgtgttca aaacatcgtt gaggcgtatc gcctgcagcg gaatgtcggc
     7501 ttcccgcttc gggtcgacgt cgacaggtac gaggaaattc tcgccgtcga tctgcaggcc
     7561 atggcccgca tagaacacga gcgcaacggt atcagggccc ttcgccgcca cttttaccgc
     7621 gaaatcgctg accttttcac gcatctcgtt ctgcgagaga tcggcagcgc tcgtcacgtc
     7681 gaatccggca tcgccaagca accgggacat ggccctggcg tcgttggccg gattgggaag
     7741 cgcgaccacg gaatggtagt ttgactggcc gatcacgagc gcaacgcgat tctcggcaaa
     7801 cgccgcgtgc gaacccagta aaataccggc ggccagaagt gcatggcgaa ggacaggacg
     7861 aaacatgggc ttacctccag gataacaagt aagtcagttg ttatcctgcg gaggttcaaa
     7921 cctcgatccg acagacccag ctatttcggt ttcttggcgg tatccgcggg ccgcgcgatg
     7981 ccccacggat catatttttc cttggaatcc gggattcttt cgagcgccgc cttgtaagcc
     8041 ttctcgtcga ccctgttttc cttctggtcg ggtttctttt catcgttgtt atggcgcttg
     8101 ccctgcatct gagcgtgcgc cggtatcgca atcaatgcca gcgtcaccgc ggcaaccgtg
     8161 agggtcttca tcgccgctct ccctggcatt ctgctgccaa acctacgcaa ccacatcacg
     8221 acaaacaagg aatcgtcgtg acggcgtaca ttaacttgtt cttctcacgc ttcagcaatt
     8281 tttgatcgcc aaggggacgc gaattgaccg cgaccgcacc caatgcaggc ccacggcggg
     8341 ataatatcgg cggatgctgc ggcaaattgt tcattcggcg ttgatggtgg gcgcggtgat
     8401 cgcgctggca ggctgcggta tcgccgacag ccgctcgccg gtgccggagt tcatgcgcgc
     8461 cggggtcagc gagccgccgc cgcccgaacc gccgcccgat gtcggccggc tgatccacga
     8521 acaactggat tcggtattca ctgccgcgtc atacccgcgc caggtgcggg tctcgccgcc
     8581 gcaccgcgag ccgtacggct ccggctggag cgcctgcgtc agggccgaac ttacgtccgt
     8641 catgggcaaa ccgctgggca ccgagaccta tcgcgtcacg atcagtggcg gcactatcgt
     8701 cgaccggcgg cgcgccgaga ccgacgataa ctgcgtatcc gagacctacc agccgatctg
     8761 atcgtcaaaa tacgccgcga cagcggtccg gctgctttca cttccggctg ctttcacttc
     8821 cggctgcttt cactttatgt ccgccagccc acgccagagc ccgcgaggcc cccaacacca
     8881 caaacgagcg cgccggccgc cgcgagatca gacagacctt ccataacgcc tccccgcgtg
     8941 taattcagcg aaggctaact tctggcataa aaatgggtgc gggtttgacc ggccgaccgc
     9001 gattccacgg aatcctgaga attgcgccca ggccaaaaat cgacgaaatg cgaggaaggt
     9061 cacagttgag ggcaattctg gatcccgggt ttcgtctggc ccggcgcccc gcgccaatga
     9121 ggggtcggct ttaaatggat gtcggaacct ggctaagcgg tctgggcctc agtcaatacg
     9181 aagccgtctt tcgtgaaagc gagatcgacg tcgacgtcct gaccgaactg accgaccagc
     9241 acctcaagga cctcggtgtt gcactcgggc acaggttgag gatgctgcgc gcgatccgtg
     9301 agcttgccgg tgacgcaccg gtcaaggcgt cggcggcgaa gccgcaagac gccgccgatc
     9361 gacgtcaatt gaccgtgatg ttctgcgatc tcgtcgattc gaccgcattg acggcgcgac
     9421 tcgatccgga ggacatgggc gacctgctcc gcgcgtttca gcgcgcggtc gcggcggcgg
     9481 taacgcgctt cgacggtcac gtcgccaagt ggaccgggga cggcgccagc gtctatttcg
     9541 gctatccccg tgcccatgag gatgacgccg aacgtgccac gcgcgccagc atcgcgctgg
     9601 tggacgcggt ggggaaactg cggcgcgagc gcggcatcgc actcgaggtg cggatcggta
     9661 tttcaaccgg cctggtcgtc gtcggcgagc tgatcggcga aggcgaggcg cgcgaacgcg
     9721 gggtcgtcgg agacaccccc aatctcgcct cgcggctgca agccctggcc gagcccgaca
     9781 ctgtcgtggt ctccgaatcg acgcgaaggt tgcttggcag gaatttcgaa ttgcagccgc
     9841 ttgggccgca ggaattaaag ggtttcaagt ccccggttcc ggcctggtcg gtcctgcgcg
     9901 tgagcgagaa cgtcaaccgg ttcgaggcgt cgcgctcggg ggccatgaca ccgttcgtcg
     9961 ggcgcgagca ggagatcgcg ctgctcatca accgatggcg ccgcgctacc aagggcgagg
    10021 gtcaggtcgc gctgttgtcg ggagaggccg gcatcggcaa atcgcgcatt ctggcggtgc
    10081 tgcgcgaacg catcggcgat gagcggcacc ttgccttccg ctatcaatgc tcgccgcatc
    10141 acggcaacga cgcgttctat cctgtcatcg gccaaatctg gcgtagcgcg ggtcttgtca
    10201 gcggcgagcc ggccgccgcg cggctcgaca aattggagat gatgatcttg cgcgcagggt
    10261 tggacatcgg cgagatcgtg ccttatctcg cgtcgctgct ggcgatcccg accgaggggc
    10321 gatatccggc gctcgaaatg tccccgagtg aggtcaagga gcggaccatt gccgcgctga
    10381 tcgcgatggt ggtcggaatt gccaaatcgg tgccggtcgt gatgctgctc gaagatgcgc
    10441 attggatcga tcccacatcg ctcgatctga ccggccagct cgtcgagaga gtgcaaaatc
    10501 ttgccgtgtt gattgtgctg acggctcggc cggagttcga ggcgccctgg atcggtcgtc
    10561 cgaatttcac cgcactcgcg ctcaaccgtt tcgaacgaag ccaggtcgtc acgatgatcg
    10621 atcgcatcat gtcaagcagg gcgctgccta ccgaggtgct cgatcagatc attgcaaaga
    10681 ccgacggcgt gccgctgttc gtggaggagc tggccaagag cgtgctggaa tccggcctgt
    10741 tgcgcgagga gaacggcgcc tatgttctcg ccgccgcgtt gacgccgctg gccatcccct
    10801 cgaccttgca cgattcgctg acggcccggc tcgatcgcct gtcgccgatc aaggagatcg
    10861 cgcagatcgg ggcgacgatc gggcgcgagt tctcctacga tctcctggaa gcggtatcgc
    10921 cggtcagagg gcaggcactg cacaacgcgc tgcagcagct catggaaacc gaactgatct
    10981 acggtcgcgg tacgccgccc aaggccagct acattttcaa gcatgcgctg gtgcaggata
    11041 ccgcctacgt gtcgctgttg cgcggccggc gtcagcgcat tcatgccgat atcgccctgg
    11101 tgctgaagga gcggtccgcc ggagaggaat gcccgcccgc catcatcgcg catcatttca
    11161 ccgaagcggg gctcgccgag caggcggcgc cgtactggct tgcggctgcc gaactggcgc
    11221 tctcgcagtc ggccccggtg gaggcggagc atcacgccag cgccggcctg gcgttgatcc
    11281 cgcgcgtgac cgaggggccg gaacgcgacg cgctcgaatt gggacttctg gtcgcccgcg
    11341 ccaacgcgat gatacctctg aaaagcattt ctgcgccgga aacattcgcc gccctggcgg
    11401 atgcgaaaca gctgctggac cggggcgtcg gcacggattt gcagcgcgtg tccattctgt
    11461 acgggctttg ctccgcctcc accctgacgg cgcgattgaa gcaggcgctc gattttgccc
    11521 atcaaatcat cgaagtcgcc gagcgccagg atgatccgac ctaccgggtc gtcggatatc
    11581 gtcagctcgg gacgcttcag ttttatgccg gaaagaaccg tgaagcgtta cagagcctgc
    11641 aaaagggtca ccaatactgc gacccacgcc ggcagcgggc gcttggctat cggttcaatt
    11701 gggatcaagg cgtcgctctg ctttccttcg aggtattggt gcggctgtcg ctcggcctgc
    11761 tcgacagcgc cgcgcaactc agcgaacggg tgcggattga actgctaagt cacgggcacg
    11821 ccgccaccgt tgcgtctgcg acatttgcct cccagacttg gcccaagctc gtgttgggtg
    11881 atcttgaggc cctggagcgc gagagcgccg aactcgcggc ctactgcgcc gaaaaaaggg
    11941 ttgagcaaat tcgtctgctc gccagctacc actacgccta cgcctgcgcc atgcgtgacc
    12001 ctgtcgagag aaacatcgcg gtccttcttg ccgcgctcga cgcgttgcgc ggatcgggtg
    12061 gaaatgccgg aaactcgatt atcaaatgca atctcgccga ggcgctgctg accgcgggag
    12121 atctggccgg agccgaggcc gttctggagg agggtttcgc cttcgtcgag caatccgggg
    12181 agcaatactg gctcgccggt ttgcatcggc tttgcgggca cgttgcgctc aaacggccgg
    12241 agccggatcg gctgcgcgcg gaagcctgtt tcaaaaaagc cattgaggtc gcccgctccc
    12301 aggaagcgcg tctgctcgaa ttgcgcgcgg ctgtcgacct cgcgcagttg tggcgcgaca
    12361 ccgggtcgga cggcgatgcc cgcgcgatgc tggagccgat cctggccgcg atcgagggtg
    12421 gcgagacgac gcgcgacgtt cgcaacgctc gcgccgcgct ggccgggctg gtttaggtgg
    12481 ggaatgatct cgatcacgcc gatgacaaga tgtcctctgc cggatcaacg aaagatttat
    12541 cgaccggccg cgatttacat tgcaaaaagc aacctcagtt tgcaaaatgc gaaaagccca
    12601 ttgatggctg gatcgacgca tcacgccaga taacaggggg tcaataatac caaacgactg
    12661 cttttatttc ttctctccgt cacgaatttc gatcattttc cggaaaaatt ccttgctgtt
    12721 ttagaccttc gatgacggcg gccgcgccgt cggggaacga attcagtgcg gactatgact
    12781 ttgcggacca tcacctcggc aatgcgccag cttcatgaag acgaagcaaa gcgttcatcg
    12841 cctatcggac ggaaatttta atcaatcgcg tgcatcacgg cgcgggctac accgcgcgat
    12901 aattttacag gagaaaccaa tgtcgccaaa tgctgatgga aacaatccga gtgaccccgt
    12961 gggcagcttt gcaaggacta ccgctcaggg cctcggcagc ttgtggggca tgatgctcga
    13021 acatctttcg gagaaacatg ccaaggccgc agcggcctgg tcggaaaacc aggaaggcgg
    13081 agtcgggcga acccccgacg gcattctggt gaccagcaag gaactggtga tgcaggttct
    13141 tctcaacgcc gacggcaatt acaccatcga gggctatcag gcgcgcatga accaaagcat
    13201 cggcctgatc tatctcggca tggattgggg accggaatac gtcaagcaat cgaccaagtc
    13261 gaacgaagcg atcggacgcg tcaccaggaa agaggcattt gattttgccc tgaaggagac
    13321 gcgcgccgcg ctggctgccc tggccgatcc cgccgacttc gacattgtga aggtttccga
    13381 caccgtcttt gcgggcatct gcaatcactg gtgggatatc cccgatggcg aatttgtcca
    13441 ggcgggcggc tttcggttgt cgaaccttgc cggaccggcg cgctgcccgg gcgattacac
    13501 cccgccttcc ggctacatcg tccatccaga cccggatttg ctgctggccc atatcgggca
    13561 gcgaaccggc cagatcctcc gagaagcggt cggcaagttc gtggcttcac ggcgcgatcc
    13621 gaaaaaccct ccggtcggcg ccatcaccaa ggcgctgttc gaggaatttc cgaaccccga
    13681 ggataacgat ctcttggcgc gaaccattgt cggggtgatg atgggcgcgc tgccgacgat
    13741 caacggcaac ctcatcacca tcgtgaagac ctggcagaaa accgcgacac tcttttccct
    13801 gcaagcaaaa ttgaaaaaaa gttcgcagcc ggacgaattt gccaaggccc atgaggtgat
    13861 cgaacagccg atgcaggaga cgatgcaaat gacgccgacg cctgatgcgc tgtggcgcat
    13921 ggcggtcaaa gaccacacgc tcggaaccac gcatccggtg caggtccgtg ccggcgacaa
    13981 gatctatctc agcgtcgtaa gggcgaccca ggaagatctc cgtaacggca tcaccgatgt
    14041 ccgtccgctg ttcggcggca atcgcagcct gacgccgcat ccgacccacg cctgcccggg
    14101 atttgaaatg gggtttggca tcctgctcgg catcgtctac gggtgcgtcg agtggcagcc
    14161 ctaaagcgcg tgccgtagca agcgtggtga gggatcgacg ccgaactggt agcgcgggcg
    14221 gtccttgcaa ctcgactttt ggctgagcgt ggtccccgcc gcccccgcga gaggcaacaa
    14281 caccgcggca acccgccgac acaagttcgg cggctctcgc tatgcctcgg tttgagacga
    14341 gctaacgcgg ggaatcgttg ccggtgtggc gcgatccagc gccggatccc gatccggtag
    14401 tcacaccaag gcccggcttg gtacctttgg cagtaccctt gtcctgcatt tcaccccgct
    14461 caccccgcgc attttcggcg gcgccctttg ctccctttgc gctgtccttg tcctgcgtcg
    14521 tctgtcccgg agcggtggat tgttgcgcca cagccggcgc gcctgcgaat actgccagag
    14581 tgccgataat tgccagatat ctgatcatcg ggatttcctc cttttacccc ccagcgataa
    14641 cgcgcctggg gaaccggggt ttccggcctt cagagttttt ccattatcgc ctgtacgcag
    14701 cgcgcagcgg cagcgatcag cagacgacga ttcatttcat ttggcatttc tcccttgccg
    14761 ccacggtttc gcggcgaagg gcaatgtcgc tgcccccccc gggagttcca cccaaccgga
    14821 aatggaccgt tggtcgccaa actccgaccg gcctaaaggc agcgcgcctg cgctctttct
    14881 ggacagagct tgaaggagtt cgacagcgaa aacagaagtc ttgcgctgcg gcttgagttg
    14941 tcgggcgcgc ggtagccaag cggcaccgcg acggcaatgt cggcccgcag atcgcctccc
    15001 agaaaaaccc gcacgccggc gccggcagag gtgagcgaaa gaccgtcggt gtacctgtag
    15061 ccgtcattcc aggccacgcc ggcgtcggca aaaccgtaaa gctgatagcc cttcaggtac
    15121 cggaaagccg aactcggtgg tgggcctgcc cgcggaaaac agggaaggac tgtcgacgac
    15181 gatcgtgcag caggccggct cgatcctgct cgaggtggaa aaacgcaacg tcagacattt
    15241 cgaggtcgag accccctacc tcgccgcggt cgtcaagggc acgcagtttc gcgtctccgt
    15301 caacgccgcc agcacccgca tcgacgtgat cagaggacag gttgaggtcg cagacttcag
    15361 gtcgggccag gttgcgcaag tgatgcctgg acaggtcgcg accgccttcg cgcggggcaa
    15421 acccggtctt tcattgagcg gaacgggcac gttcagtccg atcgagcaag gccggccgcg
    15481 agcatcgacc atccaacaga tcacggttcc gaaatccgga ctttccgcgc cgcgtcacgc
    15541 cgccaacgga cagttcaacc gcgcgctcgg acacggtgat gccggcggcg cgaggcacgg
    15601 tgtgacgcga atattggcct cgcttggtga agtcaggctg aatttccaca aggtcaccca
    15661 cggcctcgcc cgcggcgcga tttcaacgcc cggttcggcc agaagtgctg ccaacaagga
    15721 tacggtgtgg acctcccgcg aagccgcagc gacgctgggc ggcggaaacg gctccagcca
    15781 gggcaattca ggcgacggta gcagcagcac cgccaacgtc gcgaccggcg tcagcgcggc
    15841 aaccatagct gccgtcaccg ggcccggaaa cagcaacggt gacggaaacg gtaatggcaa
    15901 tggcaatgcc aacggaaacg gcaatggaaa tggcaacggc aatggccacg gcctcgcgaa
    15961 ggggcacaac aagtaaggcc gaaccatttt ttcgaaccgc tgcaccgagc atggcagtgc
    16021 gcctggccgg tcgcgaaact taggggattg ggacaacttg aaa
//
LOCUS       DASWGM010000095         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1392309,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000095 DASWGM010000000
VERSION     DASWGM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392309"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2129)
                     /locus_tag="VGA15_02990"
     CDS             complement(<1..>2129)
                     /locus_tag="VGA15_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9586693.1"
                     /translation="SAKTVIDTVALPIEQQVNGVEDMLYMQSYSGADGTYSLTVTFKI
                     GTDLNFAQVLVQNRVSSALSQLPQSVQNQGVTVQKKSTAILLFVTLTSPNATYDSLFL
                     SNYATINLRDELSRLSGVGNVTVFGAGQYSMRVWLDPNKLQVRGLMPQDVIQAIQQQS
                     QQVTAGQVGMPPTPAGQAFQYTLNVNGRLDETSEFEDVIVKTGNNGDVTRVRDVGSVE
                     LGAQTYSQIFSLNKHPATGIGVFQSPGANALEVEKAVRKKMATLATAFPQDVKFDIPF
                     DTTKFVSASIDEVYKTLIEAGFLVLVVILIFLQDWRAMLVPATTVPVTIIGAFAAMAA
                     LGFTVNLSTLFAIVLAIGIVVDDAIVVVEGAAHNIEQGMSGHDAAIKAMDALFAPIVG
                     ITLVLISVFLPAAFLPGLTGRMYAQFALVIAATALLSAVNAATLKPTQCALWLRRPVP
                     PEQRNWFYRGFNAVYDRLERGYAGLIGRMVGHSGLSVISALIVIGIAGYGLSRVPTGF
                     IPIEDQGYLLAAVQLPDGAALDRTQRVLDSVSEMAGKAPGVEQVISIAGISALDGSSS
                     LANAGVAYLILKDWSERGPGEDLRSLVVGLNQKLGAIPEARILVIPPPPIQGIGNAAG
                     FAMQIELRDGNSDFGKLQAITAAVVSSAQTQSALQRVSSSFRSMVPQFDVEIDRVKTQ
                     TLHVTTDQVFSTLASYLGSTYVNQFNK"
BASE COUNT          405 a          658 c          674 g          392 t
ORIGIN      
        1 cttgttgaac tgattgacat aggtcgatcc cagataagag gccagcgtcg agaacacctg
       61 gtcggtcgtg acatgcaggg tctgggtctt gacccggtcg atctcgacat cgaattgcgg
      121 caccatcgag cggaatgacg aactcacgcg ctgcagcgcg ctctgggtct gcgcactgga
      181 aaccacggcg gcggtaatgg cttgcagttt gccaaagtcg gagttgccgt cgcgcagttc
      241 gatctgcatc gcgaaaccgg cggcgttgcc gatgccttgt atcggcggcg gcggaatcac
      301 aaggatccgc gcttccggga tcgcgccaag cttctggttg aggccaacga ccagcgaccg
      361 caaatcctcg ccgggcccgc gctcgctcca gtccttgagg atcagatagg caacgccggc
      421 gttggcgagg ctcgaactgc cgtcgagggc cgatatgccg gcgatgctga tcacctgctc
      481 gacgcctggc gccttgccgg ccatttcgct gacggaatcg agcacgcgct gggtgcggtc
      541 gagcgcggcc ccgtcgggca attgcaccgc ggccagcagg tagccctggt cctcgatcgg
      601 gatgaagccc gtgggcacgc gcgacaggcc gtaaccggca atgccgatca cgatcagcgc
      661 ggagatcacc gacaggccgc tgtgcccgac catgcggccg atcagcccgg cataacccct
      721 ttcgagacgg tcatacaccg cgttgaagcc gcgatagaac cagttgcgtt gttcgggcgg
      781 aaccggccga cgcagccaca gcgcgcattg ggtcggcttc aatgtcgcgg cgttgacggc
      841 gctgagcagc gcggtcgcgg cgatcaccag cgcgaattgc gcgtacatgc gcccggtgag
      901 ccccggcaaa aaagccgccg gcaggaacac cgagatcagc accagcgtga tgccgacaat
      961 cggcgcgaac agcgcatcca tcgccttgat cgcggcgtca tggccggaca tgccctgctc
     1021 gatattgtgg gcggcgcctt cgaccacgac gatggcgtcg tcgaccacga tgccgatcgc
     1081 gagcacgatc gcaaataatg tcgacaggtt gacggtgaac ccgagcgccg ccatcgcggc
     1141 gaacgcgcca atgatggtga ccggcaccgt ggtcgccggc accagcatcg cgcgccagtc
     1201 ctgcaggaag atcaggatca cgaccagaac cagaaagccg gcctcgatca gcgtcttgta
     1261 gacttcgtcg atcgacgccg agacgaattt ggtggtgtcg aacggaatgt cgaatttcac
     1321 atcctgcgga aaggctgtgg ccagcgtcgc catttttttc ctgaccgcct tctcgacctc
     1381 gagcgcgttg gcgccgggcg actggaatac gccgatgccg gtggcgggat gcttgttgag
     1441 tgagaatatc tggctgtagg tctgcgctcc cagttcgacc gatccaacat cgcgcacccg
     1501 ggtgacatcg ccgttgttgc cggttttgac gatgacatcc tcgaattcgc ttgtctcgtc
     1561 cagccggccg ttgacgttca gcgtatactg aaatgcttgc cccgcgggcg tcggcggcat
     1621 gccgacctgg cccgcggtga cctgctggct ctgctgctgg atcgcctgaa tgacgtcctg
     1681 gggcatcaac ccgcgcacct gcagcttgtt cggatcgagc cagacccgca tcgaatactg
     1741 accggcgccg aacaccgtca cgttgccgac ccctgataac cgcgacagtt catcgcgcag
     1801 gttgatcgtg gcgtagttgg agagaaaaag actgtcgtaa gtcgcgttcg gggaagtcag
     1861 cgtcacgaat aagagaatcg ccgtggactt cttctgcacg gtgacgccct ggttctgcac
     1921 cgattgaggg agctgcgaca acgcgctcga cacccggttc tgcaccaata cctgcgcgaa
     1981 gttcagatca gtgccgatct tgaaagtaac cgtgagcgaa taggtgccgt cggcgccgct
     2041 gtaggactgc atgtagagca tgtcctcgac gccgttgacc tgctgctcga tcggcagtgc
     2101 cacggtatcg atgacggtct tggcgctgg
//
LOCUS       DASWGM010000096         4038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_8854, whole
            genome shotgun sequence.
ACCESSION   DASWGM010000096 DASWGM010000000
VERSION     DASWGM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8854"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VGA15_02995"
     CDS             complement(<1..169)
                     /locus_tag="VGA15_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586694.1"
                     /translation="MSTRTFGEPIKRNEDRRLLTGQALFIDDIELPGMLHVAFLRSQV
                     AHAKIVSIDVSS"
     gene            complement(173..658)
                     /locus_tag="VGA15_03000"
     CDS             complement(173..658)
                     /locus_tag="VGA15_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9586695.1"
                     /translation="MDSDFHDITLSVNGKQYQRRVESRLLLSDFIRHDLALAGTHVGC
                     EHGVCGACTVLLDGQPIRSCLMLAVQARGHEISTVEGLTDKDGEYHPLQQAMHDHHGL
                     QCGYCTPGILMTMTAFLDENASPTEDDVREALSGNLCRCTGYQNIVDAVMTAAMRTRG
                     R"
     gene            complement(648..1523)
                     /locus_tag="VGA15_03005"
     CDS             complement(648..1523)
                     /locus_tag="VGA15_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9586696.1"
                     /translation="MKPASFKYVAAASVDHALSLKAEHGDDAKFLAGGQSLMPAMNFR
                     LAQPAVLIDINDIADLTGIRRSAAATQVGPVTRYRALQRDAAFARDFALIGEALPHIA
                     HPQIRNRGTIGGNLAHADPASELPAIALALGARFHVRTATQERWIEASDFFVGALTTD
                     LQPDEMLVGIELVLPKPRTGCCFMEIARRRGDFAIVGVAAMMTLGERDECTDVRLTFC
                     GVGETPIDASSAAEALVGCAPTEEAIRDVAASVHRMIDPGGSVHAAADYQRHVAGVLT
                     ERALRTAHQRVRDGQ"
     gene            1853..2677
                     /locus_tag="VGA15_03010"
     CDS             1853..2677
                     /locus_tag="VGA15_03010"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586697.1"
                     /translation="MNKPADVTAESVALTQRLAVDVRNVSLTFETADGKVDALSNVSL
                     QIADGEFVSFIGPSGCGKTTMLRVIADLQQPTAGTLLVNGMSAEQARLARRYGYVFQA
                     PALFPWRTIEKNLKLPLEIMGFSDSEQRQRAARYLALVNLTGFERKFPWQLSGGMQQR
                     VSIARALSFDPELLLMDEPFGALDEIVRDHLNEQLLQLWDKTGKTVLFVTHSIPEAVF
                     LSSKIVVMSPRPGRIIDIIDCNFPRDRTLEIRETPEFLKIAQRVRVGLRAGHSYDE"
     gene            2667..3593
                     /locus_tag="VGA15_03015"
     CDS             2667..3593
                     /locus_tag="VGA15_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586698.1"
                     /translation="MTSDMLALPASGIGTLRRHPLVVRYAPIVTIVLALIAIWYVAAV
                     LMNLSLVRDAFEREETPYTVSELIAGTMSAERPLLPAPHQVITAFVDSVFGYAPTAPR
                     SLVYHSTVTLSATLLGFALGALFGIGLALMIVHSRVLEKSLLPWIICSQMVPILALAP
                     IFIVVLGAMGLQGLLPKSIISAYLCFFPITIGMVKGFTAPDPMQLDLMRTWSASPRQV
                     LAKLRWPSAVPYLFASLKVAITISLIGAIVAELPTGAEAGIGARLLAGSYYGQTIQIW
                     AALLASAILASGLIGLVDLTERIVGLRMGARP"
     gene            3590..>4038
                     /locus_tag="VGA15_03020"
     CDS             3590..>4038
                     /locus_tag="VGA15_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586699.1"
                     /translation="MNPRRLRLGFILAAAGMLCAVVLPLSGEAVATLPARPIWLALTL
                     ASALLFAVAAWTGGSSAIAPGFAGVLCGAATTLALLRDPGVGGSAAAGFWAMVLAVWL
                     GAAFCVIQLASMRPRDDASRRALNLAIPLLFGAVVFYLWEVLVRGFGV"
BASE COUNT          665 a         1248 c         1310 g          815 t
ORIGIN      
        1 cgctggaaac atcgatgctg acgatcttcg catgcgcgac ctggctgcgc aggaacgcga
       61 catgcagcat ccccggcaac tcgatgtcgt cgataaacag cgcctggccc gtcagaagcc
      121 tgcgatcttc attgcgtttg atcggttcgc cgaatgtgcg ggtggacatc tctcatcgac
      181 cccgcgttcg catcgcggca gtcatgacgg catcgacgat gttctgataa ccggtgcagc
      241 ggcacagatt gcccgacagc gcttcacgca cgtcatcctc agtcggcgat gcgttttcat
      301 cgagaaacgc cgtcatcgtc atcagaatgc cgggcgtgca atagccgcac tgcaggccgt
      361 gatggtcgtg catcgcctgc tgcagcggat ggtattcgcc atctttgtcg gtgagaccct
      421 caacggtcga aatctcgtgc ccccgggcct gcaccgccag catcaggcac gagcgtatgg
      481 gttgcccgtc gagcagcacc gtgcaggcgc cgcagacgcc gtgctcgcag ccgacatggg
      541 tgccggccag cgccagatcg tggcgaataa agtcgctcag cagcaggcgc gattcgacgc
      601 ggcgctgata ttgtttgccg ttcaccgata gcgtgatgtc atggaaatca ctgtccatcg
      661 cgcacccgct ggtgggccgt ccggagcgcg cgctcggtga gcacgcccgc gacgtggcgc
      721 tgataatccg ccgcggcgtg cacactgcct cccggatcga tcattctatg cacggacgct
      781 gcgacatcgc ggatcgcttc ctcggttggc gcgcatccga cgagagcctc ggcggccgaa
      841 cttgcgtcga taggggtttc gcccacgcca caaaacgtca gccgaacatc ggtgcattca
      901 tcccgttcgc ccagcgtcat catggcggcc acgccgacaa tggcaaaatc gccgcgccgg
      961 cgcgcgatct ccatgaagca gcaaccggtt cgcggcttcg gcagcaccag ttctataccc
     1021 accagcattt catcgggctg cagatccgtc gtcagcgcgc cgacaaagaa gtccgacgcc
     1081 tcgatccatc gttcctgtgt tgccgtccga acgtggaacc gggcccccag cgccaaggcg
     1141 atggcgggaa gctccgaggc cggatcggcg tgcgcaagat ttccgccgat ggtgccgcga
     1201 ttgcggatct gcgggtgcgc gatatgcggc agcgcttcgc cgatcaaggc aaaatcgcgc
     1261 gcaaaagccg cgtcgcgctg cagcgcacgg taccgcgtga ccggcccaac ctgggtcgcc
     1321 gcggcggacc gacgaatacc ggtgagatcc gcgatgtcgt taatgtcgat caagaccgcc
     1381 ggttgagcca ggcggaaatt catcgccggc atcaggcttt gaccgccagc caggaatttc
     1441 gcgtcgtcgc catgttccgc cttgagcgag agcgcgtgat cgacggaggc ggcggctacg
     1501 tatttgaagg aggcaggctt catggcgctc gcgtgcaggc aatcgaaacg cggttcggcg
     1561 cgccgaagcg cgaagccgca gcgaaactat gaggcggccc ccggcgcaag tcaaaatcga
     1621 agcgcttttt tgctatctga aaatccgata ggggcatgtt tgaccccgat ggttgttctt
     1681 gcccattaat atctccgcgt gcgtaaacat tgagcgcgcc atttgcagcg gcgtttgtca
     1741 gtatttatcc atggcgttgc aagtgccgaa gtcattgatg ccggataatc cgcgcgttac
     1801 gctgcgaccg ggcgggcgag aattgttcgt cactccgatc gaaacggtac gcatgaacaa
     1861 gccagcggat gtcacggctg aatcagttgc gctgacacag cgcctcgcgg tcgacgttcg
     1921 caatgtttcg ctgacgttcg agactgccga cggcaaggtc gatgcgctgt cgaatgtcag
     1981 tttgcagatc gccgacggcg agttcgtttc ctttatcggc ccttccggct gcggcaagac
     2041 cacgatgctg cgggtgatcg ccgacctgca gcaaccgacg gccggcacgc ttctggtcaa
     2101 cggcatgagc gcggagcagg cgcgtctggc gcgcagatat ggctacgtgt tccaggcgcc
     2161 ggcattgttc ccgtggcgca cgatcgaaaa gaacctgaag ctgccgctgg agatcatggg
     2221 attttccgat agcgagcagc ggcagcgggc ggcgcgctac ctggcgctgg tcaatctcac
     2281 gggcttcgag cgcaagtttc cctggcagtt gtccggcggc atgcagcagc gggtttcgat
     2341 cgcgcgcgcg ctgtcgttcg atccggaact gctgctgatg gacgagccgt tcggtgcgct
     2401 cgacgagatc gtccgcgacc atctcaacga gcagttgctg caattgtggg acaagaccgg
     2461 aaagacggtg ctgttcgtca cccactcgat tcccgaggcg gtgttcctgt cgagcaagat
     2521 cgtggtgatg tcgccacggc ccgggcgcat catcgatatc atcgattgca attttccgcg
     2581 cgaccgcacc ctggaaatcc gcgagacgcc ggaattcctc aagatcgcgc agcgcgtgcg
     2641 cgtcggcctg cgggcgggac attcctatga cgagtgatat gctggcgctg cctgcatcgg
     2701 gtatcggcac gctgcggcgt catccgctgg tggtgcgcta cgcgccgatt gtcacgatcg
     2761 ttctggcgct gattgcgatc tggtatgtcg cggcggtgct gatgaattta tcgctggtgc
     2821 gtgacgcctt cgagcgggag gagacgccgt acaccgtctc ggagctgatt gcgggcacca
     2881 tgagcgccga gcggccgctg ctgccggcgc cgcaccaggt gattacggcc ttcgtcgatt
     2941 cggtcttcgg ttacgcgccg accgcgccgc gcagcctcgt ctatcattcg acggtaacgc
     3001 tgtcggcgac gctgcttggc ttcgcactgg gcgcgctctt tggtatcggt ctggcgctga
     3061 tgatcgtcca cagccgcgtg ctggagaaaa gcctgctgcc ctggatcatc tgttcgcaga
     3121 tggtcccgat cctggcgctg gcgccgatct tcattgtggt gctcggcgcc atgggattgc
     3181 agggcctgct gccgaagtcg atcatttcgg cctatctgtg tttcttcccg atcacgatcg
     3241 ggatggtgaa gggttttacc gcgcccgatc cgatgcagct cgacctgatg cggacatggt
     3301 cggcgtcacc ccgacaggtg ctggcaaagc tgcgctggcc gtcggccgtg ccctatctgt
     3361 tcgcgagcct caaggtcgcg atcacgatct ccctgatcgg cgccatcgtg gctgaactgc
     3421 cgaccggcgc cgaagctggt attggcgcgc ggctgcttgc gggctcctac tatggccaga
     3481 ccatccagat atgggcggcg ctgctggcgt cggcgatcct cgcctcggga ctgatcggac
     3541 tggtggacct gaccgagcgt atcgtcggat tgcgaatggg tgcacggcca tgaacccgcg
     3601 caggctgaga ttgggattca tcctcgccgc ggcaggcatg ctgtgcgccg tggtcctgcc
     3661 gctctcgggc gaggcggtcg ctacgttgcc ggcgcggccg atatggttgg cattgacgct
     3721 cgcctcggcg ctgctgtttg cggttgccgc ctggaccggc ggatcgtcgg cgatcgcgcc
     3781 tggttttgca ggcgttctat gcggagccgc gaccacgctg gccttgttgc gcgatcccgg
     3841 cgtcggcggc tcggccgcgg cggggttttg ggcgatggtg cttgcggtct ggctcggcgc
     3901 ggcattttgc gtgatccagc ttgcctcgat gcgtccgcgc gacgacgcat cgcgccgcgc
     3961 cctcaacctt gcgattccgc tgctgttcgg agcggtggtg ttctatctct gggaagtgct
     4021 ggtgcgcggc ttcggcgt
//
LOCUS       DASWGM010000097         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2641448,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000097 DASWGM010000000
VERSION     DASWGM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641448"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1289)
                     /locus_tag="VGA15_03025"
     CDS             complement(81..1289)
                     /locus_tag="VGA15_03025"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX9586700.1"
                     /translation="MTETKSSNAGSVEVWITGIGLATSLGEGLDANWEALNARRINVD
                     EKGFAPYIVHPLAPVNLDAQIPKKGDQRQMEAWQRIGTYAAGLALASAGVKGNKEILG
                     RMDMIVAAGGGERDLVVDCNILNMEAKGNCAPGFLNERLMNDLRPTLFLAQLSNLLAG
                     NIAIVHGVTGSSRTFMGEEAAGVDAARIALARIAAGQSDIALIGGSQNGERKDLLVLY
                     EFDDFNLRDKFAPVWARQKNAGFALGSAGAFLVIESKAHARARGAKPYARLTNVVADL
                     AHRKHPGEVTATLEKLWSKLGKLGDKGAIITGATGAEPVTSEERAFLGEHPDFAVRAS
                     GTMFGHTMETQFPLGLGLAALSISRGALFPPNDPTGLEVEISKPPTQIVVIGAGHWRG
                     EGMALVEAIS"
     gene            complement(1282..>1516)
                     /locus_tag="VGA15_03030"
     CDS             complement(1282..>1516)
                     /locus_tag="VGA15_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HEX9586701.1"
                     /translation="KMRSFVSPGELLMVEANVVHEGSGYAITAAKVRVGGELRCNATI
                     TFGIVPFPNPELRVHMDAMAKRIGFPQQAITHD"
BASE COUNT          270 a          493 c          458 g          295 t
ORIGIN      
        1 atgccggtga cgacgacgat cggcctgccg aatttatcgc gcgtagctgt catgttttcc
       61 ccgcccgccc agcgccgtgc tcaactgatc gcctcgacca gggccatgcc ttcgccccgc
      121 caatgaccgg ccccgatgac gacaatctgg gtcggcggct tggatatttc aacctccagc
      181 ccggtgggat cgttcggcgg aaacaatgcc ccgcgtgata tcgagagtgc ggcgaggccc
      241 agtccgagcg gaaactgggt ttccatggtg tgcccgaaca tggtgccgct ggcccgcacc
      301 gcaaaatccg gatgctcgcc caggaacgcc cgctcctcgg aagtgaccgg ctcggcgccg
      361 gttgcgccgg tgatgatggc gcccttatcg ccaagcttgc cgagcttcga ccacagtttt
      421 tccagcgttg ccgtgacttc gccggggtgc ttgcggtgcg ccagatcggc gacgacgttg
      481 gtcaggcgcg cataaggttt cgcgccgcgc gcccgtgcat gggctttgga ctcgatgaca
      541 agaaacgcgc cggccgagcc aagtgcgaaa ccggcgtttt tctggcgcgc ccaaaccgga
      601 gcaaatttgt cccggaggtt gaaatcgtcg aattcataga gaaccagcaa atccttgcgc
      661 tcgccgtttt gcgagccgcc gatcagcgcg atgtcgctct ggccggcggc gatccgcgcg
      721 agcgcgatcc ttgctgcgtc aacgcccgcg gcttcctcgc ccatgaaggt gcgcgacgac
      781 ccggttacgc cgtggacgat ggcaatgtta ccggccagca ggttcgaaag ctgcgccaaa
      841 aaaagcgtcg ggcgcagatc gttcatcagc cgctcattga gaaacccggg agcgcagtta
      901 cctttggctt ccatattcaa aatgttgcag tccaccacga gatcgcgctc gccgccgccg
      961 gcggcgacga tcatgtccat ccgccccagg atttccttgt tgcccttcac gccggccgaa
     1021 gccagtgcca gcccggcggc ataggttccg atccgctgcc aggcttccat ctggcgctgg
     1081 tcgcctttct tggggatctg cgcgtccagg tttaccgggg ccaagggatg aacgatgtag
     1141 ggcgcgaaac ccttctcgtc gacgttgatg cgccgcgcat tgagcgcctc ccagttggcg
     1201 tccagcccct cgccgagcga ggtcgcaaga ccgataccgg tgatccagac ttccaccgat
     1261 cccgcgttcg aagacttcgt ctcagtcatg ggtgatagcc tgctgcggaa agccgatgcg
     1321 tttggccatc gcgtccatgt gcacgcgcag ttccggattc gggaaaggga caataccgaa
     1381 cgtgatcgtg gcgttgcatc ttaattcgcc gccaacgcgg accttggcgg ccgtaatcgc
     1441 ataacccgac ccttcgtgaa cgacgttcgc ctcgaccatg agcagttcgc cggggctgac
     1501 gaaacttcgc atcttg
//
LOCUS       DASWGM010000098         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_700011,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000098 DASWGM010000000
VERSION     DASWGM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700011"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /gene="eda"
                     /locus_tag="VGA15_03035"
     CDS             complement(<1..501)
                     /gene="eda"
                     /locus_tag="VGA15_03035"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX9586702.1"
                     /translation="MTATPKQEELAALFKQAVVIPVLTIERLEDAIPLAKALVAGGVR
                     VLEVTLRTPVAIPAAQAIISSVPEAVVGIGTILNADDLARAEALGAVFGISPGATPDL
                     LKAAAASALPFAPGMATASELMQALAHGFNLVKFFPAEQSGGIKALRALAGPFPDARF
                     CPTGGIG"
     gene            complement(498..1442)
                     /locus_tag="VGA15_03040"
     CDS             complement(498..1442)
                     /locus_tag="VGA15_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX9586703.1"
                     /translation="MTRVACIGECMIELKQAKGGLYSRGYGGDTLNTSVYLARLGVDV
                     DYVTALGDDTLSDEMIAGWTAEGVGTKRVARLPGKLPGIYMIGTDDKGERRFFHWRES
                     AAARALMDLPETDDILNSLASYDVVYLSAITLSIYSENGRKRLMAALKRARLLGTRVA
                     FDTNFRARGWPDLNMARSVFSDAFEAADIVLASTEDLLPLYPGEGNATLMVGISSPEV
                     VLKLSEPASVLRFAGGSHEVKAEPLTKPAVDTTAAGDSFAAAYIAARLAGSDPIDAAR
                     AGHRLAGVVVGYPGAIIPRSAMPVRKTPRSAPSRKASP"
     gene            complement(1494..2480)
                     /gene="denD"
                     /locus_tag="VGA15_03045"
     CDS             complement(1494..2480)
                     /gene="denD"
                     /locus_tag="VGA15_03045"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEX9586704.1"
                     /translation="MHILVLGAAGMVGRKLTERLLRDGRLGDREISRMTLHDVVAPTK
                     PTNAAIPISVVTSDFADPNTAASLVAHRPDVIFHLAAIVSGEAELDFDKGYRINLDGT
                     RMLLDAIRLAGGGYKPRVVFTSSIAVFGAPFPEAIGDEFFQTPLLSYGTQKAICELLL
                     ADYSRRGFMDGIGIRLPTICIRPGLPNKAASGFFSNILREPLAGKEAVLPVSEDVRHW
                     HASPRSAVGFLIHAATMDTATAGARRNLTMPGLSATVGEEIAALKRVAGDKVAARIKR
                     EPDPFIMGIVDGWPRNFDAKRAVKLGFTTAEQTFDDIIKIHIEDELGGKFAA"
     gene            complement(2612..3550)
                     /locus_tag="VGA15_03050"
     CDS             complement(2612..3550)
                     /locus_tag="VGA15_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9586705.1"
                     /translation="MTDLPAPKPVNLKAVLSGSSPTAAKDDHSEGMSYLESVPRRLVT
                     LYLPLSIIVIILLFPFYWMGLTAIKPDEQLLDLDKFNPFWTWSPTFKHIYKLLFQSYY
                     PHWLWNTMYVAVCATVLSIFASVLAAYAIVRLRYRGAHWVGGLIFLSYLVPPSILFIP
                     LATVVFQYGLFDSPLALILTYPTILIPFSTWLLMGYFKTIPFELEECALIDGASRWQI
                     LIKIVLPLAIPGLISAFIFCFTLCWNEFIYALTFLQSTQNKTVPVAIVNEFVDGDIYR
                     WGSLMAGALAGSLPLVILYAFFVEHYVSAMTGAVKE"
     gene            complement(3543..>4043)
                     /locus_tag="VGA15_03055"
     CDS             complement(3543..>4043)
                     /locus_tag="VGA15_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9586706.1"
                     /translation="ISWSLKHLGLITQNINFLGDVTWARICVIFVNIWRGVPFVAITL
                     LAGLQTVQPSLYEAATLDGASKWEMFRFITYPLLTPIIAVVMTFSVLFTFTDFQLIWA
                     MTRGGPVNATHLMATLSYQRAIIAGQLGEGAAISSAMIPFLLAAIMISWFGLQRRKWQ
                     QGESND"
BASE COUNT          807 a         1239 c         1316 g          681 t
ORIGIN      
        1 gccgatgccg ccggtcggac aaaaccgcgc atccggaaaa ggcccggcca gggcccgaag
       61 tgccttgata ccgccggact gctcggccgg gaagaatttc accaggttga aaccgtgggc
      121 cagcgcctgc atcagttcgg acgccgtcgc catgcccggc gcgaacggca aggcgcttgc
      181 ggcggcagcc ttgagcaggt cgggcgtggc gccgggactg atgccgaaga cggcgccgag
      241 cgcttccgcg cgggccagat cgtccgcgtt caggatcgtg ccaatcccga cgacggcttc
      301 cggcacgctt gagatgatcg cctgtgcggc cggaatcgca accggcgtgc gcagcgtgac
      361 ttccagtacc cgcacgccgc ccgcaaccag tgccttggcg agcgggatcg cgtcttcaag
      421 ccgttcgatg gtaagaaccg ggatgacgac ggcctgcttg aacagcgcgg cgagctcttc
      481 ctgttttggt gtcgcggtca tggagaggcc ttgcgagagg gtgccgatcg cggcgttttc
      541 ctgaccggca tggcactgcg cggaatgatc gcgccgggat agcccacgac cacgccggcg
      601 agacgatgtc cagcgcgcgc ggcgtcgatc gggtccgagc ccgccagccg agcggcgatg
      661 taggccgctg caaaactgtc gcccgccgcg gtggtgtcga cggcaggttt tgtcaggggc
      721 tcggccttca cctcgtgcga accgccggca aaccgcagga cgctggcggg ctcgctgagc
      781 ttcaacacca cttccggact ggaaatgccg accatcaggg tcgcgttgcc ttcgccggga
      841 tagagcggca gtaaatcttc cgtcgaagca agcacgatat ccgcggcctc aaaggcatcg
      901 ctaaagacgc tccgcgccat gttgagatcg ggccagccgc gggcgcgaaa attggtatcg
      961 aacgcgacgc gggtcccgag cagccgcgcg cgtttgagag ccgccatcaa gcgctttcgg
     1021 ccgttctcgc tgtagatcga cagcgtgatc gcggagagat agacgacgtc gtagcttgcg
     1081 agcgaattca ggatgtcgtc ggtttcgggt aagtccatca gggcgcgcgc agccgcgctt
     1141 tcgcgccagt ggaagaaccg ccgctcaccc ttgtcgtcgg tcccgatcat gtagatgccc
     1201 ggcaatttgc ccggcagccg cgcgacgcgc ttggtcccga cgccttcggc ggtccagccc
     1261 gcgatcatct catcgctcag ggtatcgtcg ccgagcgccg tgacataatc gacgtcaacg
     1321 ccaaggcggg cgagatagac tgatgtgttc aacgtatcgc ccccatagcc gcgcgaatac
     1381 aggccgccct tggcctgttt cagctcgatc atgcattcgc cgatgcacgc tacccgggtc
     1441 atgacagcgc cacgccagcg aagccagctg tatccgcttc gcccaacacc acgttaagcc
     1501 gcgaacttgc cgccgagttc gtcttcgatg tgaatcttga tgatgtcatc aaacgtctgt
     1561 tcggcggtgg tgaagccgag tttcacggcc cgcttggcgt cgaaattgcg tggccagccg
     1621 tcgacaatgc ccatgatgaa cggatcgggt tcgcgcttga tccgcgccgc caccttgtcg
     1681 cccgccaccc gcttcaacgc ggcgatctcc tcgccgacgg tcgccgaaag cccgggcatg
     1741 gtgaggttgc ggcgtgcacc ggcggtcgcg gtatccatgg tcgcggcatg gatcaggaaa
     1801 ccgaccgccg agcgtggcga ggcatgccag tgccggacgt cctccgacac cggcagcacc
     1861 gcttccttgc cggcaagcgg ctcgcgcagg atgttggaaa agaaccccga ggccgccttg
     1921 ttgggcagac cgggacggat gcaaatggtc ggcagccgga tgccgatgcc gtccatgaac
     1981 ccgcggcgcg aataatcggc cagcaggagt tcgcagatcg ccttctgggt gccgtagctc
     2041 aacagcggcg tctgaaagaa ctcgtcaccg atggcctcgg gaaacggcgc gccgaacacc
     2101 gcgatcgacg aggtgaagac gacgcgcggt ttgtagccgc cgccggccag ccggatcgca
     2161 tcgagcagca tccgcgtgcc gtcgagattg atgcgatagc ccttgtcgaa atcaagctcg
     2221 gcctcgccgg agacgatcgc ggcgagatgg aagatcacat cggggcggtg cgccaccagc
     2281 gatgccgcgg tattgggatc ggcgaagtcg cttgtgacga cgctgatggg gattgccgca
     2341 ttcgtgggct tcgtcggagc cacgacatcg tgcagcgtca tccgcgaaat ctcgcggtcg
     2401 ccgaggcgcc cgtcgcgcaa cagccgctcg gtgagtttgc gcccgaccat gccggcggcg
     2461 ccgagaacca gaatatgcaa gacccaactc cttgttcttg ttctatagcg ttttcgagcg
     2521 aagcatgccc tcggacttga tccgggggtg ggaaccggtt cgcgtcaaga aaacgcgtca
     2581 aaataagaga tcagacgcgg gccctgcgcg cttattcttt gacggctccg gtcattgccg
     2641 aaacatagtg ctcgacaaag aacgcgtaaa gaatgaccag cggcagcgag cccgccagtg
     2701 cgcctgccat cagcgatccc cagcggtaga tgtcgccatc gacgaattcg ttgacgatgg
     2761 cgaccggcac cgtcttgttc tgggtcgatt gcaggaaggt cagcgcgtag atgaattcgt
     2821 tccagcacag ggtgaagcag aaaatgaacg ccgagatcaa gccgggtatg gccagcggca
     2881 gcacgatctt gatcaggatc tgccagcggc tcgcgccgtc gatcagcgcg cattcctcca
     2941 gctcgaacgg gatggtcttg aaatatccca tcaagagcca ggtcgagaac gggatcagga
     3001 tggtcggata ggtcaggatc agcgccagcg gcgagtcgaa caggccgtac tggaacacga
     3061 cggtggccag cgggatgaac aggatcgacg gcggcaccag ataggacagg aagatcagcc
     3121 cgccgaccca atgcgcgccg cggtagcgca gccgcacgat ggcataggcc gccagcacgc
     3181 tggcgaagat cgacagcacg gtggcgcaga ccgccacata catcgtgttc cagagccaat
     3241 gcggataata gctctgaaac agcaatttgt agatgtgctt gaaggtcgga ctccaggtcc
     3301 agaacggatt gaacttgtcg agatcgagca gctgctcgtc cggtttgatc gccgtcaggc
     3361 ccatccagta gaacggaaac agcaggatga tgacgatgat cgacagcggc agatagagcg
     3421 tgacgaggcg gcgcggcacc gactccagat agctcatgcc ctcgctgtga tcgtccttcg
     3481 cggccgtcgg cgacgagccg gacagtacgg ccttgagatt gacgggcttt ggtgcgggaa
     3541 gatcagtcat tggattcccc ttgctgccac ttgcggcgct gcagcccgaa ccaggaaatc
     3601 atgatcgcgg cgagcaggaa cgggatcatc gcgctcgaga tcgcggcgcc ctcgccgagt
     3661 tggccggcga tgatggcgcg ctgatacgac aaggtcgcca tcagatgggt ggcgttgacc
     3721 ggtccgccgc gggtcatcgc ccagatcagc tggaagtcgg tgaaggtgaa cagcaccgaa
     3781 aacgtcatca cgaccgcgat gatcggggtc agcagcggat aggtgatgaa gcggaacatc
     3841 tcccatttgc tcgcgccgtc cagcgtcgcc gcctcgtaga gcgaaggctg cacggtctgc
     3901 aagcccgcca gcagcgtgat cgcgacaaag ggcacgccgc gccagatatt gacgaagatc
     3961 acgcagatcc gcgcccaggt gacgtcgccg agaaaattga tgttctgggt gatcaggccg
     4021 aggtgcttga gcgaccatga aat
//
LOCUS       DASWGM010000099         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_840052,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000099 DASWGM010000000
VERSION     DASWGM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840052"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1532)
                     /gene="gor"
                     /locus_tag="VGA15_03060"
     CDS             complement(144..1532)
                     /gene="gor"
                     /locus_tag="VGA15_03060"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX9586707.1"
                     /translation="MAEFDVDLFVIGGGSGGVRAARIAAGYGARVMVAEEYRMGGTCV
                     IRGCVPKKLFVIGSHIQHEIEDAAGFGWLIPQVDFDWPTLVANKDKEIARLEGIYTSN
                     VEKSGARIVKTRAVFEDAHTLRLATGETVKAKYVLIATGSAPNHGPAIPGIEHVISSN
                     EVFHLKELPKRIVIQGGGYIALEFACIFAGFGTDVTVIYRGDNILRGFDEDVRAHVRA
                     EMERQGITILTGCTVNKVDKPGKEFISHLSNGSSIASDLVMFAIGRHPNVANLGLENA
                     GVAINSNNGGIAVDGWSQTSAPNIYAIGDVTHRINLTPVAIREGHAFADTVFGKRPVQ
                     VDHTNIPTAVFSQPEVGTVGLTETQARAQFSHVDIYKTDFRPIKSTMSGRDTRILMKL
                     VVDGTTDRVVGCHIVGDTAAEIVQAVGIAVKMKATKADFDATFALHPTAAEELVTMRT
                     PTARYVREAAAE"
     gene            complement(1616..>1758)
                     /locus_tag="VGA15_03065"
     CDS             complement(1616..>1758)
                     /locus_tag="VGA15_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEX9586708.1"
                     /translation="LGQKLLASEPRAIQFSMSYMNQWAQSFAEVVNGQFRAEMRKRGK
                     EI"
BASE COUNT          330 a          543 c          526 g          359 t
ORIGIN      
        1 ttgccgagtg gctcgccaat ggtccctatc atgagtggag gctacaaagt tacaagtgcg
       61 tcatgggcaa atacattccc aaaggcgaag catgaagtgc gcgccaaccc tcttatgagg
      121 ggttggcttt tggtaggcga ttcctactcc gcggccgcct cacgcacata gcgcgccgtc
      181 ggcgtccgca tggtcacgag ttcttcggcc gcggtcggat gcagcgcaaa cgtggcgtcg
      241 aaatcggcct tggtcgcttt cattttgacg gcaatgccga cggcctgcac aatctcggcg
      301 gcggtgtcgc cgacgatgtg gcaaccgacc acgcgatcgg tggtgccatc gaccaccagc
      361 ttcatcagga tgcgggtgtc gcggcctgac atcgtcgact tgatcggccg aaaatcggtc
      421 ttgtagatat cgacgtggct gaactgcgcg cgcgcctggg tctcggtcaa tccgacggtg
      481 ccgacctccg gctgggaaaa caccgcggtc ggaatgttgg tgtggtcgac ctgaaccgga
      541 cgcttgccga acacggtatc ggcgaaggcg tggccttcgc ggatcgcgac cggcgtcagg
      601 ttgatgcgat gggtgacatc cccgatcgcg tagatgttcg gtgccgaggt ctgcgaccag
      661 ccgtcgaccg cgatcccgcc attgttggaa ttgatggcga cgccggcgtt ctcgagaccg
      721 agattggcga cattgggatg ccgtccgatc gcgaacatca ccagatcgga cgcgatgctg
      781 gaaccattcg agagatgcga gatgaattcc ttgccgggct tgtcgacctt gttgaccgtg
      841 cagccggtca aaatggtgat gccttgccgc tccatctcgg cgcgcacatg ggcgcggacg
      901 tcctcatcaa aaccgcgcaa gatgttgtcg ccgcgataga tcaccgtcac gtcggtgccg
      961 aagccggcaa aaatgcaggc aaattccagt gcgatatagc cgccgccctg gatcacgatg
     1021 cgcttcggca attccttcag gtgaaacact tcgttcgacg agatcacgtg ttcgatgccc
     1081 ggtatcgccg gtccatgatt gggcgcgctg ccggtcgcga tcagcacgta tttcgccttc
     1141 acggtctcgc cggtggcgag gcgaagggtg tgggcatctt cgaacaccgc gcgcgtcttg
     1201 acgatgcgcg cgccggactt ttcgacattg ctggtgtaga tgccctccag ccgcgcgatc
     1261 tccttgtcct tgttggcgac cagcgtcggc cagtcgaagt cgacctgcgg gatcaaccag
     1321 ccaaagcccg cggcgtcctc gatctcgtgc tggatatgcg atccgatcac gaacagcttt
     1381 ttcggcacgc agccgcggat cacgcaggtg ccgcccatcc ggtattcttc ggccaccatc
     1441 acccgcgcgc catagcccgc ggcgatccgg gcggcgcgaa ccccgcccga acctcctccg
     1501 atgacgaaca gatcgacgtc gaactcagcc atatccaccc cgagcgaaat cggattcttt
     1561 ggatcagatt tccacttcag atttgcgcat cagatttaca cgtcagattt acacgtcaga
     1621 tttccttgcc gcgcttgcgc atctcggcgc ggaactgacc gttgacgacc tcggcaaaac
     1681 tctgcgccca ctggttcata taggacatgc tgaactgaat agcccggggc tcgctcgcca
     1741 gcagcttctg gccgagcg
//
LOCUS       DASWGM010000100         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_700099,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000100 DASWGM010000000
VERSION     DASWGM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700099"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VGA15_03070"
     CDS             <1..622
                     /locus_tag="VGA15_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX9586709.1"
                     /translation="LVKKGRQTFDAATKHIYDPQPSDWTGRFELLLIGNGEDRDASRE
                     ALKNAGFGSPLPGVWVAPSGVPVPEEAARAIRLEVSAEDDSGRRLLSESWPLDRTADA
                     YLKFMKTFEPLCGWISRRERLTDVDAFTARILLIHYYRRVVLRDPLLPTALLPADWPG
                     RAARQLCGEIYRGLLAASEQWLDRNGTNEAGPLPKAGEELARRFGG"
     gene            700..1701
                     /gene="paaA"
                     /locus_tag="VGA15_03075"
     CDS             700..1701
                     /gene="paaA"
                     /locus_tag="VGA15_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX9586710.1"
                     /translation="MYTQALNSSEAEGQTIKPGIEDATRAAQFQACIDADDRIEPNDW
                     MPAAYRKTLTRQISQHAHSEIVGMLPEGNWITRAPSLRRKAALLAKVQDECGHGLYLY
                     AAAETLGASREELVDLMLAGKAKYSSIFNYPTLTWADIGAIGWLVDGAAIMNQIPLCR
                     CSYGPYARAMIRVCKEESFHQRQGFEIMLTLSRGSEEQKAMAQSALDRWWWPVLMMFG
                     PPDKASQHSDTSTRWKIKRFSNDELRQKFVDATVPQAQYLGLTIPDPGMKQNAAGNWE
                     YSAIDWNEFNAVLAGNGPCNRERLAARRKAHDEGAWVREAAQAYAEKRKQRLNAQAA"
     gene            1720..2007
                     /gene="paaB"
                     /locus_tag="VGA15_03080"
     CDS             1720..2007
                     /gene="paaB"
                     /locus_tag="VGA15_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX9586711.1"
                     /translation="MATPNTPLWEVFIRSRNGLAHKHVGSLHATDATLALQAARDVYT
                     RRGEGLSIWVVPSSAITASDPSEKGMMFEPAESKIYRHPTFYDVPDEVGHM"
     gene            2007..2783
                     /gene="paaC"
                     /locus_tag="VGA15_03085"
     CDS             2007..2783
                     /gene="paaC"
                     /locus_tag="VGA15_03085"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX9586712.1"
                     /translation="MAIASIQVSETPLVLYALRRADDALILGHRLSEWCGHAPMLEED
                     MALANMGLDLLGQARELYSYAADVEGKDNDEDKFAYLRDVRQYRNLLLLEQPNGDFAH
                     TMVRQFLYAGFADLYWRAMMTSGDATLAAIAAKSEKESAYHLRHSSEWIVRLGDGTEE
                     SHARAQTAIDDLWAFTGEMFEVDDSERALIDAGIAVDPKGLRPQWLKTVLSVVSEATL
                     ALPKSDWMQQGGRSGQHSEHLGHLLSELQLMQRTFPGVAW"
     gene            2777..3280
                     /gene="paaD"
                     /locus_tag="VGA15_03090"
     CDS             2777..3280
                     /gene="paaD"
                     /locus_tag="VGA15_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX9586713.1"
                     /translation="MVTAVFSDAGLRQRAWDTAAKVVDPEIPVLTIADLGVLRDVAVN
                     GGCVEVVITPTYSGCPAMNMITLEIELALEREGFRNSKVRTVLSPAWTTDWMSEDGRR
                     KLREYGIAPPQAANSRRALFGVQQVACPQCGSGDTEVLSEFGSTSCKALWRCKSCREP
                     FDYFKCH"
     gene            3283..>3521
                     /locus_tag="VGA15_03095"
     CDS             3283..>3521
                     /locus_tag="VGA15_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9586714.1"
                     /translation="MSSSIPRFHRLAVNDLRRESPDAVSLTFAIPQALADDYRFAPGQ
                     YLTLRTTMDGAEVRRSYSICSGPDDGELRIAVKKV"
BASE COUNT          670 a         1081 c         1136 g          634 t
ORIGIN      
        1 gctggtgaag aaagggcggc aaacctttga tgccgcgacc aaacatatct acgatccgca
       61 accgtccgat tggaccggac ggtttgagct tcttttgatc ggcaacggtg aggatcgcga
      121 cgcttcccgc gaggcgctga aaaatgccgg ttttggcagc ccgctgccgg gcgtctgggt
      181 ggcgccgtcg ggcgtgcccg ttccggagga ggcggcgcgc gcgatccgtc tggaagtttc
      241 cgccgaggac gacagcggac gccgactgct cagcgagagc tggccgctcg atcgcaccgc
      301 cgacgcctac ctgaaattca tgaaaacgtt cgagccattg tgcggctgga tcagccgtcg
      361 cgaacgcctt acggatgtcg acgcgttcac ggcgcgcatt ttgttgatcc attactaccg
      421 gcgggtagtg ctgcgcgatc cgctgctgcc gacggcgttg ctgcctgcgg attggccggg
      481 aagggcggcg cgccagctct gcggcgaaat ttaccgcggg ctgcttgccg cgtcggagca
      541 atggcttgac cgcaacggta ccaacgaggc cggccccctg ccaaaggccg gcgaagagct
      601 ggcgcgccgg ttcggcggtt aggcgggata tgttacaaaa atctattgag tttcatgtat
      661 tttgttatat gtttagggtc gcctccccgg agatcggcca tgtacaccca ggcgctcaac
      721 tcgtcggaag ctgaaggcca aactatcaag ccaggtatcg aggatgccac acgcgcggcg
      781 cagtttcagg cgtgcatcga cgccgacgat cgcatcgagc cgaacgactg gatgccggcg
      841 gcctatcgca agacgctgac gcggcagatt tcgcagcacg cgcattccga aatcgtcggc
      901 atgctgccgg aaggcaactg gatcacgcgt gcgccgtcgc tgcgccgcaa ggccgcgctc
      961 ttggccaagg tgcaggacga gtgcggtcac gggctctatc tctatgccgc cgccgagacg
     1021 ctcggcgcct cgcgcgagga actggtcgat ctgatgctgg ccggcaaggc gaagtactct
     1081 tcgatcttca attatccgac gctgacctgg gcggatatcg gcgcgatcgg ctggctggtc
     1141 gacggcgcgg cgatcatgaa ccagattccg ctgtgccgct gttcctatgg tccctatgcg
     1201 cgcgccatga tccgcgtctg caaggaggaa tcctttcacc agcgccaggg ctttgagatc
     1261 atgctgacgc tgtcgcgcgg ctccgaggag caaaaggcga tggcgcagag cgcgctggat
     1321 cgctggtggt ggccggtgct gatgatgttc ggcccgcccg acaaggccag ccagcacagc
     1381 gacacctcga ccagatggaa aataaaacgc ttctccaatg acgagctgcg ccaaaagttc
     1441 gtcgatgcca ccgtgccgca ggcgcaatac ctcggcctga cgattcccga tcccggcatg
     1501 aagcagaacg ccgcgggcaa ctgggaatac agtgcgatcg actggaacga attcaacgcc
     1561 gtgctcgccg gcaacggccc ctgcaaccgc gaacgcctgg cggcgcggcg caaggcgcat
     1621 gacgaaggcg cctgggtccg cgaggcagcc caggcctacg ccgaaaaacg caagcaaaga
     1681 cttaacgcgc aagccgcata aaaattcagg gaggtcatga tggccacgcc gaacacgccg
     1741 ttgtgggaag tcttcattcg cagccgcaat ggcttggcgc acaagcatgt cggctcgctg
     1801 cacgcgactg acgcgacgct ggcgctgcag gccgcgcgcg acgtctacac ccgccgcggc
     1861 gaggggctgt cgatctgggt ggtgccgtca agcgcgatca ccgcctccga tccatccgag
     1921 aagggcatga tgttcgagcc cgcggaatcc aagatctacc gccatccgac attttacgat
     1981 gtgcccgacg aagtcggaca catgtgatgg caatcgcctc gatccaggtc tccgaaaccc
     2041 cgctggtgct gtacgcgctg cgccgcgccg acgacgcctt gattctgggg catcggctgt
     2101 cggaatggtg cggccatgcc ccgatgctgg aagaggacat ggcgctcgcc aacatgggcc
     2161 tcgatctgct cggccaggcg cgcgaacttt attcctacgc cgctgatgtc gaaggcaagg
     2221 acaacgacga ggacaaattc gcgtacctgc gagacgtcag gcagtaccgc aacctcttgc
     2281 tgctggaaca gccgaacggc gatttcgcgc acaccatggt gcggcagttt ctttacgcag
     2341 gcttcgccga cctctattgg cgcgcgatga tgacatcggg cgacgccacg ctggcggcga
     2401 tcgcggcgaa atccgagaag gaaagcgcct atcatttgcg gcattcgtcg gaatggatcg
     2461 tccggctcgg cgacggcact gaggaaagcc acgcccgcgc gcagaccgcg atcgacgatc
     2521 tctgggcttt taccggcgag atgttcgaag ttgacgacag cgagcgggcc ttgatcgatg
     2581 ccggtatcgc ggttgatcct aagggcttgc gtccgcaatg gctcaagacg gtcttgtccg
     2641 ttgtgagcga agctacattg gcgctgccca agagcgactg gatgcagcag ggcggccgca
     2701 gcggccagca tagcgagcat ctcgggcatc tgctcagcga attgcaattg atgcagcgga
     2761 cctttccggg ggtcgcatgg tgaccgccgt gtttagcgat gccgggctgc ggcaacgcgc
     2821 gtgggatacc gcggccaaag tggtcgatcc cgagattccg gttttgacca tcgccgatct
     2881 cggcgtgctt cgcgacgtcg cggtgaatgg tggttgcgtc gaggtcgtga tcacgccgac
     2941 ctattccggc tgccccgcaa tgaacatgat cacactggaa atcgagctgg cgctggagcg
     3001 cgagggcttt cgcaattcaa aagtccgcac cgtgctgtcg ccggcctgga ccaccgactg
     3061 gatgagcgaa gacggccggc gcaagctaag ggaatatggc atcgcgccgc cgcaggccgc
     3121 gaactcccgc cgcgcgctgt tcggcgtgca gcaggtggcg tgcccgcaat gcggctccgg
     3181 ggataccgag gtgctgtcgg aattcggctc gacctcctgc aaggcgctgt ggcgctgcaa
     3241 gagctgccgc gaaccgttcg actatttcaa atgtcattga acatgtcgtc atcgatacct
     3301 cgctttcatc gcctcgccgt caacgatctg cgccgcgaat cgcccgacgc ggtgtcgctg
     3361 acgtttgcga ttccgcaggc gcttgcggac gactaccggt ttgcccccgg acagtatctg
     3421 accttgcgca cgacgatgga cggcgcggaa gtgcgccgct cctattcgat ctgctccggc
     3481 cccgatgacg gcgagttgcg catcgcggtg aaaaaggtcg a
//
LOCUS       DASWGM010000101         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2502361,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000101 DASWGM010000000
VERSION     DASWGM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502361"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VGA15_03100"
     CDS             complement(<1..1191)
                     /locus_tag="VGA15_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX9586715.1"
                     /translation="MTQVRDLVMMAAVMGKLSTIFLLALLTLNLISNRVTVADEPPRS
                     GIENPAALQNFFRALADARSRQRLEPVRIMHFGDSHTAADVLTADIRRHFQGDFGDGG
                     AGWIVPKNPMSTRRLGVTSGATSGWSIEGIGGRIAPHRIYGPAGIALSTSQPNERAWV
                     EASGNHFELYYVRQPGGGRIDVLVDGRSVLDAPLSLSSPMPEASHLFFDTPAANGRHR
                     IEIRTMSEGQVSILGIVSEHIAPGVVYDVLGVNGARARRILSWNQPALAAVLTDRKPD
                     LIVLEYGTNEVVDNGWTVASYQRFLAGIMRRLQSAAPQASLIVLGPPDRSDLPVAVNR
                     MPLLIEAQRRAAFQAGAAFWSSYDVMGGAGTMNTWVAQGLGQGDHVHLTRSGYNRIAD
                     IFYQD"
     gene            complement(1296..>1550)
                     /locus_tag="VGA15_03105"
     CDS             complement(1296..>1550)
                     /locus_tag="VGA15_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586716.1"
                     /translation="YPKEWDAYAGHYRNDSPWFGSTRIFVRKGKLTAEGTPLTPLGDG
                     VFRAGAEEWSPERISFGPVVDGRATRMKFSGIQFYRTFTP"
BASE COUNT          307 a          474 c          481 g          288 t
ORIGIN      
        1 gtcctggtag aaaatgtccg cgattcgatt gtagccggac cgcgtcaaat gaacgtggtc
       61 gccctggccg agaccctgcg ccacccaggt gttcatggtg ccggctccgc ccatgacatc
      121 gtatgaactc caaaacgccg cacccgcttg gaaggccgcg cggcgctgag cttcgatcag
      181 taacggcatt cgatttaccg cgacgggcag gtcgctgcgg tccggcgggc cgagcacgat
      241 cagcgacgcc tgcggggccg cgctctgcaa tctgcgcata atgccggcga gaaagcgttg
      301 gtaagaggcg acggtccaac cgttgtcgac aacttcattg gtgccgtatt cgagcacaat
      361 cagatcgggt ttgcggtcgg ttagcacagc cgccagggcg ggttggttcc aactgaggat
      421 gcgacgggcg cgcgccccgt tgacaccgag tacgtcatag acgacgccgg gcgcgatgtg
      481 ctccgaaacg attcccaaaa tgctgacctg gccctcgctc atcgtgcgaa tttcgatgcg
      541 gtgccggccg ttcgcagcgg gcgtatcaaa gaaaagatgc gaagcttcag gcattggcga
      601 cgatagtgac aggggagcat cgagcacgct ccggccgtcg accaacacat cgattctgcc
      661 gccgccgggt tggcgcacgt agtacaactc gaaatgattg ccggaggcct cgacccaggc
      721 gcgctcattc ggctgacttg tgctcagagc gattccggcc gggccataga ttcgatgcgg
      781 cgcgatgcgt ccgccgattc cctcaatcga ccaacccgaa gttgcgccgc tcgtgacacc
      841 cagccggcgc gtgctcatcg gattcttcgg gacaatccag ccggcgccgc catcgccaaa
      901 atcgccctga aagtggcgcc gaatatctgc ggtcaggacg tctgcggccg tatgcgaatc
      961 gccaaagtgc atgatgcgca ccggctcgag tctctgtcgc gatcgcgcat ccgccagtgc
     1021 gcgaaaaaag ttttgcagtg ccgccgggtt ttcaatgcca ctccggggcg gctcgtcggc
     1081 caccgtcacg cggttagaga tgagattgag cgtgaggaga gcgagtagaa aaattgtcga
     1141 tagtttcccc atgaccgcag ccatcatcac gaggtcgcgg acctgggtca atgtggtcac
     1201 ccacactttt gagtcagaac cgggagcggt agcgaccgga tcataaaatc aagcagagtg
     1261 aagatcccgg tcgctaccgc tctcggttct gacagtcacg gcgtaaaggt gcggtaaaac
     1321 tgaatgccgg agaatttcat gcgcgtggcg cggccatcaa ccaccgggcc gaagctgatg
     1381 cgctctggcg accattcttc cgcgccggcg cggaacactc catcgccgag cggggtgagc
     1441 ggcgtgccct ccgcggtcag ctttcccttg cgcacgaaga tccgcgtgct gccgaaccac
     1501 gggctgtcgt tgcggtaatg tccggcgtac gcgtcccatt ctttcggata
//
LOCUS       DASWGM010000102         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_700145,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000102 DASWGM010000000
VERSION     DASWGM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700145"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VGA15_03110"
     CDS             <1..430
                     /locus_tag="VGA15_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9586717.1"
                     /translation="WRKVLRINLDGPFICCKAIVPSMVKQNYGRIVNIASIAGKEGNP
                     NAAHYSASKAGLIALTKSLGKELAGYEIAVNAVTPAAAKTAIFDQLTQAHIDFMLSKI
                     PKGRFVLVEELAAMVAWLASEDCAFSTGAVFDISGGRATY"
     gene            572..1252
                     /locus_tag="VGA15_03115"
     CDS             572..1252
                     /locus_tag="VGA15_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX9586718.1"
                     /translation="MNISLYDVSVWVLPLVIAITFHEAAHGFVAHHFGDNTAFELGRV
                     SFNPLRHIDPFGTLVLPAILLLSHSPFLFGYAKPVPVNFRALRNPRLDMVWVALAGPA
                     TNIALALLAALAFHVLVFAPASTAQWLADNLKNAFVINIVLAIFNMLPIPPLDGGRVA
                     VGLLPNILAVPLARLEPFGMLILIGTLIILPMLGNQVGLNLDIISTILRTATGYVIRL
                     LLFVTGNA"
     gene            1310..1714
                     /locus_tag="VGA15_03120"
     CDS             1310..1714
                     /locus_tag="VGA15_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586719.1"
                     /translation="MMIARRADTKARSDFATWKMMAKLNGASSLPAEAQAFLVSYKAL
                     LEQMTEAEATEAAIGIVYKSYYGEMGGIGVPPDVKARSNDQVTDNVTTFKRPPAQRPK
                     TAAGSGAQPRLPVALIFACLVVVYVGIRYFWR"
     gene            1865..>2133
                     /locus_tag="VGA15_03125"
     CDS             1865..>2133
                     /locus_tag="VGA15_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586720.1"
                     /translation="MKRSIAAVLLTTGLVFATGLVFALVFASGFASGALAQGKLDKVV
                     KVGSLGDQSGLYSDIGGPGSSVAAQMAIEDSGLLAKGWKIDLISA"
BASE COUNT          401 a          657 c          608 g          467 t
ORIGIN      
        1 atggcgcaag gttttgcgca tcaatctcga cgggcccttc atctgctgca aggcgatcgt
       61 gccttcgatg gtcaagcaga attacggccg catcgtcaac atcgcctcga tcgccggcaa
      121 ggaaggcaac cccaacgcgg cgcattattc ggcttcaaag gccggcctga tcgcgctaac
      181 aaagtcgctc ggcaaggaac tcgccggtta cgaaatcgcc gtcaacgcgg taaccccggc
      241 cgcggccaaa accgcgatct tcgatcagct gacccaagcg catatcgact tcatgctgtc
      301 gaagattccg aaagggcgtt ttgttctggt ggaagaactg gcggcgatgg tggcgtggct
      361 ggcgtcggag gattgcgcgt tctccaccgg cgccgtgttc gacatttccg gcgggcgggc
      421 gacttactag cgcgagcatg atcctcccgg aaaatcggtt tccatttttc gggatctcgc
      481 cccgcaacat caactcgcgg tgttcggggc acatcatgcc gttcatttgt ggtagcgtcg
      541 ccgcattcgt cgaaccggtc aggacatcag attgaacatc tcattatacg acgtatcggt
      601 ctgggttttg ccgctcgtca tcgccattac gtttcatgag gcggcccacg gcttcgtcgc
      661 ccatcacttc ggcgacaaca ccgccttcga actcggccgc gtcagcttca atccgctcag
      721 gcacatcgac ccgttcggca cgctggtgct gccggcaatc ctgctgctgt cccactcgcc
      781 gtttctgttc ggctatgcca agccggtgcc ggtgaacttc cgcgcgcttc gcaatccgcg
      841 gctcgacatg gtctgggtgg cgctggccgg accggctacc aatatcgcgc tggccctgct
      901 cgcggcgctg gcgtttcatg tcctggtctt cgcacccgcg agcacggcgc aatggcttgc
      961 cgacaacctc aaaaatgcgt ttgtcatcaa tatcgttctt gcgatcttca atatgcttcc
     1021 gattcctccg ctggacggcg ggcgggtcgc ggtcggattg ctgcccaaca tactggcagt
     1081 cccgcttgcc cggctggaac cgtttggcat gctgatcctg atcgggactt tgattattct
     1141 gccaatgctt ggtaaccagg tcggtctaaa tctcgatatt atttcgacga tactgcggac
     1201 agcgaccggc tatgtgatac ggttgctgct cttcgtaacc ggcaatgcgt aatcgaaccc
     1261 cagcgagccg ccaaaaaggc ctgaggccat cttgatcagg gctgccgaca tgatgatcgc
     1321 ccgacgcgcc gacaccaagg cccgctcgga ttttgccacc tggaaaatga tggcgaaact
     1381 gaatggtgcc tcctcgctgc ccgccgaagc ccaggcgttc ctggtcagct acaaggcgct
     1441 tctcgagcag atgacggagg ccgaggcgac cgaagcagcc atcggcatcg tttacaagag
     1501 ttactatggc gaaatgggcg gcatcggcgt accgcccgat gtcaaagccc gttcgaacga
     1561 tcaggtcacg gacaacgtca ccaccttcaa gcgcccgccg gcccagcggc cgaagaccgc
     1621 ggctggctcg ggagcccagc cccgacttcc cgtcgccctg atattcgcgt gcctcgtcgt
     1681 cgtctatgtc gggattcgat atttttggcg gtgacgccaa acgtccagcg gaattctccg
     1741 cagcctgaac tactccgacc tgcgcggacc actcgtcgcc tcggctatgc gtcgaatgca
     1801 aggcggggca tgcgttccac gatttattct tgtgttagtt caaatcaata ttttgggagg
     1861 tgaaatgaag cgatcaatcg cagccgtgct gctgaccact ggtctcgtat ttgcaactgg
     1921 tctcgtattt gcactcgtat ttgcaagtgg ctttgcaagt ggcgcgctcg cacagggcaa
     1981 gctcgacaaa gtcgtcaagg tcggcagtct cggcgaccag tcgggtctct actccgacat
     2041 tggcggcccg ggctcgtccg tcgccgccca gatggcgatc gaggattccg gactattggc
     2101 caagggctgg aagatcgatc tgatctccgc gga
//
LOCUS       DASWGM010000103         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1811278,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000103 DASWGM010000000
VERSION     DASWGM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811278"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..432
                     /locus_tag="VGA15_03130"
     CDS             37..432
                     /locus_tag="VGA15_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX9586721.1"
                     /translation="MRKFFTALVLIPLGLVFVIFAVANRHLVTVSFDPFNSSDPSVGF
                     RLPLFVVIIGVAIAGVAAGGSATWFRQRHWRRAARQHEADARQARAQLADLRAGAMTP
                     SRYDPQRLAAPLHGGGYGATGRDKQGATL"
     gene            487..1164
                     /locus_tag="VGA15_03135"
     CDS             487..1164
                     /locus_tag="VGA15_03135"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX9586722.1"
                     /translation="MSLIVKICGLSTRETLDVALDAGADMVGFVFFPPSPRHLSLETA
                     RDLGGRVKRRAVKVALTVDADDATLGNIVEALQPDVLQLHGKETVARLRDIKQTFGLP
                     VMKAIAVETVADLASLPGYAAVADRILFDARAPKGATRPGGLGAVFDWHVLEQLDLKL
                     PFMVSGGLSAENVAEAVRVTRAGGVDVSSGVEGAPGVKDPGMIRAFIRAARIAEQDTA
                     LAVAGGR"
     gene            1325..2539
                     /gene="trpB"
                     /locus_tag="VGA15_03140"
     CDS             1325..2539
                     /gene="trpB"
                     /locus_tag="VGA15_03140"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX9586723.1"
                     /translation="MNPNLPNSFRSGPDERGHFGIFGGRFVAETLMPLILDLEQAYAD
                     AKADPAFQAEMNGYLKHYVGRPSPLYLAERLTEHLGGAKIYFKREELNHTGSHKVNNV
                     LGQIMVARRMGKKRIIAETGAGQHGVATATLCARFGLECVVYMGAVDVERQQPNVIRM
                     EMLGAQVIPVQSGSRTLKDAMNDALRDWVTNVHNTFYCIGTVAGPHPYPMMVRDFQSI
                     IGVETRAQMLAAEGRLPDSLIACIGGGSNAMGLFHPFLDDPGIEIFGVEAAGHGLTQL
                     HAASIAGGRPGVLHGNRTYLLMDDDGQIQDAHSISAGLDYPGIGPEHSWLHETGRVKY
                     LSATDDEALAAFQLLSRLEGIIPALEPAHAIAKVMELAPKRSKDHLMVVNLSGRGDKD
                     IPQVVDILRGKK"
     gene            complement(2622..2966)
                     /locus_tag="VGA15_03145"
     CDS             complement(2622..2966)
                     /locus_tag="VGA15_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX9586724.1"
                     /translation="MDKFKAGARKLRSSQTSAEAKLWQALRNRRLARWKFRRQHSIDR
                     YVVDFVTLDGKLIVEVGGVTHAEPSQLARDKARSDVLEACGFHVVRVSNTDAYDNLEG
                     VLEMIESTLRLD"
     gene            3039..>3557
                     /gene="trpA"
                     /locus_tag="VGA15_03150"
     CDS             3039..>3557
                     /gene="trpA"
                     /locus_tag="VGA15_03150"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9586725.1"
                     /translation="MTTRIDARFAELKKQGRSAFITFLMAGDPDPATSLDIIRALPKA
                     GSDIIEIGMPFTDPMADGPAIQAAGLRALKAGMTLKKTLAMVRAFRKDDNTTPLVLMG
                     YYNPIYIYGVDKFLIDAKSAGVDGLIIVDLPPEEDSELCLPAMKAGLNFIRLATPTTD
                     DKRLPAVLANTSG"
BASE COUNT          647 a         1130 c         1110 g          670 t
ORIGIN      
        1 aagctggtac tatctccagt aacgcgagag atggtcatgc gaaaattctt cacggcgctg
       61 gtgctgattc cgctgggcct ggtctttgtc atcttcgcgg tcgccaatcg tcatctggtg
      121 acggtgtcgt ttgacccgtt caactccagc gacccgtcgg tggggttcag gctgccgctg
      181 ttcgtcgtca tcatcggggt cgcgatcgcg ggcgtggcgg caggcggttc cgcgacctgg
      241 ttccggcagc gccattggcg gcgcgcggca cgccaacacg aggccgatgc gcggcaggcg
      301 cgggcgcaac tggccgattt gcgcgccggc gccatgacgc cctcccgcta cgatccgcag
      361 cggctggccg ctcccttgca cggcggcgga tatggcgcca ccgggcgaga caagcagggc
      421 gcgacgttgt agaacgcggg ccacaagcgc accgcttcac tgccgcccca aaccgtgccc
      481 tggaacatgt ccctgatcgt caaaatttgc ggcctgtcca cgcgcgagac tcttgacgtc
      541 gcgctggatg ccggcgccga catggtggga ttcgtgtttt ttccgccttc gccccggcat
      601 ctgagcctgg agacggcgcg cgacctcggc ggccgggtaa agcgccgcgc cgtaaaagta
      661 gccctcacgg tcgatgccga cgatgcaacg ctcggcaaca tcgtcgaggc cctgcagccc
      721 gacgtcctgc aattgcatgg caaggaaacc gtggcgcggc tgcgcgacat caagcagacg
      781 tttggcttgc cagtgatgaa ggctatcgcg gtcgagactg tggccgacct cgcttcattg
      841 cccggctacg ccgccgtggc cgatcgcatt ctgtttgacg cccgcgcccc caagggcgcc
      901 acccgtcccg gcgggcttgg tgcggtgttc gactggcatg tgcttgagca gctcgatctc
      961 aagctgccgt tcatggtctc gggtggcctc tcggctgaaa atgtcgcgga ggcggttcgc
     1021 gtcacccgcg ccggtggcgt cgatgtttcc tcgggcgtgg agggcgcacc cggtgtcaag
     1081 gatcccggga tgattcgcgc ctttattcgc gcggcgcgga tcgctgagca agacacagct
     1141 ctcgcggttg cgggcggtcg atgaacgcgg cgaaccattt aacgtcgtca tgcccgggca
     1201 cagtagtctg ccttgcgcag actacgtata cttgtctgcg ctcccgggca tccacgtctg
     1261 ttgaaactag aaccaggcaa gacgtggatg gccgggacaa gcccggccat gacgaaaggc
     1321 gacgatgaat ccaaatttac caaactcctt ccgcagcggt cccgacgagc gcgggcattt
     1381 cggcattttc ggcggacgct ttgtcgcgga aacgctgatg ccgctgatcc tcgatctgga
     1441 gcaggcttac gccgacgcca aggccgatcc ggcgttccag gctgaaatga acggctacct
     1501 caagcactat gtcggcaggc cgtcgccgct gtatctcgcc gagcgcctga ccgaacatct
     1561 cggcggcgca aagatctatt tcaagcgcga ggagctgaac cacaccggct cgcacaaggt
     1621 caacaacgtg ctcggccaga tcatggtggc gcggcggatg ggaaagaagc gcatcatcgc
     1681 cgaaaccggc gctggccagc acggcgtcgc caccgcgacg ctgtgcgcgc ggttcgggct
     1741 tgaatgtgtt gtctatatgg gtgcggtcga tgtcgagcgg cagcagccca atgtcatccg
     1801 catggagatg ctgggcgccc aggtgattcc ggtgcagtcc ggttcgcgca cattaaagga
     1861 cgcgatgaac gacgcgctgc gcgactgggt caccaatgtg cacaacacgt tctactgcat
     1921 cggcaccgtg gcgggcccac atccctatcc gatgatggtg cgagacttcc agtcgatcat
     1981 cggcgtcgag acccgtgcgc agatgctagc tgccgaaggc cgcctgccgg attcgctcat
     2041 cgcctgcatc ggcggcggct ccaacgcgat ggggctgttc catccgttcc tcgatgatcc
     2101 cggcatcgag atctttgggg tcgaagccgc gggccatggg ctgacgcaat tgcatgcggc
     2161 ttccatcgcc ggcggccggc ccggcgtgtt gcacggcaac cgcacttatt tgctgatgga
     2221 cgatgacggc cagatccagg acgcgcattc gatttcggcc gggctggatt atcccggcat
     2281 cggccccgag cattcttggc tgcacgaaac cggccgggtg aaatatctgt ccgcgaccga
     2341 cgacgaagcg ctggcggcgt ttcaattgct gtcgcggctg gaaggcatca tcccggcgct
     2401 ggagcccgcg catgccattg ccaaagtgat ggagctcgca ccgaagcgat ccaaagatca
     2461 tctgatggtg gtcaaccttt ccggccgcgg cgacaaggac atcccccaag tggtggatat
     2521 tttgcggggg aagaagtgac cattaaacgg cgcctcttcc ttcacctctc cccgccgggg
     2581 agaggtcggc tgcgtagcag ccgggtgagg ggccttttct ttcaatcaag cctaagtgtg
     2641 ctttcgatca tctcgaggac accctccaga ttatcatacg catctgtatt tgagacgcgg
     2701 acaacgtgga agccgcaggc ctccagcaca tcggaccggg ccttatctcg tgccaactgc
     2761 gatggctcag cgtgcgtcac gccgccaact tcgacgatca gctttccgtc gagcgttacg
     2821 aaatcgacga cgtaccgatc gattgaatgc tggcgacgaa atttccaccg cgccagccgc
     2881 cgattgcgca acgcttgcca aagctttgcc tcggcacttg tttgcgacga gcgaagtttt
     2941 cgggctccag ccttgaattt gtccatcccc ctcacccggc tgctgcgcag ccgacctctc
     3001 cccggcgggg agaggtatag cacagcgaac gcagcaatat gaccacccgc atcgacgcac
     3061 gctttgccga gttgaagaaa caaggccgct cggctttcat taccttcctg atggcgggcg
     3121 atcccgatcc agcgacctcg ctcgacatca tcagggcgct gccgaaggct ggcagcgaca
     3181 tcatcgagat cggcatgccc ttcaccgatc cgatggcgga cggtccggcg atccaggcgg
     3241 cggggctgcg cgcgctcaag gccggcatga ccctgaaaaa gacgctcgcg atggtccgcg
     3301 cgtttcgcaa agacgataac accacgccgc tggtgctgat gggctactac aatccgatct
     3361 acatctacgg cgtcgacaaa tttctcatcg atgcgaagtc cgcgggcgtc gatggactga
     3421 tcatcgtcga cctgccgccg gaggaagaca gcgaattgtg cctgccggcg atgaaggccg
     3481 ggctgaattt catccggctg gcgacgccga ccaccgacga caagcggctg cccgccgtgc
     3541 tcgcgaacac ctcgggc
//
LOCUS       DASWGM010000104         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_840281,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000104 DASWGM010000000
VERSION     DASWGM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840281"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            832..1827
                     /locus_tag="VGA15_03155"
     CDS             832..1827
                     /locus_tag="VGA15_03155"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX9586726.1"
                     /translation="MDQIFSGTKAFQAPILARSLGQLATSFGGFFATCTAMYLCLAVS
                     LWITLPMSALAAGFLVRIFIIQHDCGHGSFFRSRWANELIGSLCSLMTLTPYAFWRRQ
                     HARHHGSWNNLDRRAASGLDIYSSCMTVAEYRALGRWRRCLVRLTNHPIVANLLLPPL
                     VFVILYRVPFDAAKGWRHERLGVYLTNLALADFIGGLGLAVGYGRVAAVQLPIMVIAS
                     IVGVWLFSIQHRFEHAQWMPEASWSFAAASFRGTSYLRLPRPLQWFTGNIGFHHVHHV
                     DPRIPNYRLEECHNADAGFQSASTLTLRTALQALRYALWDSDLERLVPFRFAAAD"
BASE COUNT          429 a          861 c          781 g          525 t
ORIGIN      
        1 gggctccgcc cgtccgccgc ccggtgacat atgtgcccca tccccggccc taacggatca
       61 aagcgtgccc cgccgctcga atgctaaccg ccgatcggct aggcctgcgc gcggtcgaca
      121 aattccgatc ggagcgtacc aacactctac tacatccatc acgccggcac tggcgagggg
      181 gcgtgccgcc tccatgttca gagatgcgcc ctgtctggtt ctgcgcgaca tcgagaagct
      241 gacacgatgc tcgcttcgcg cgatcccagt cccgtcggca actcccgccc gcgcaatggc
      301 ccgatcgcgc gaccggcggc atcaagcctg ccgcgggaag caccctcgcg gcaggctcca
      361 gcaagcggcc gagccgcccc ggcgcgacag cgccgcggcc taccagcacc gaattccggc
      421 gcggagaacc gcccgctgcc agttcgcttc cggcattcct gcgccgcccg tcgccaacca
      481 aacctggttc ggccaaacct ggttcgacag gtcgccgcgg cgaacagcga ggccgcactg
      541 ctgccactcg gttcgatggt gcgcgctaga cgcatgcagc acggaatccg tgacatgctg
      601 atcgcacgcg aaggcgagga gatcgtctgc ccgaagggca cggtctgcgg tcgcattatg
      661 cgcgacgcca acgaccaaat cattgacgag gatttttgcc gtgcgtgaaa tttccttttc
      721 gccccgcgac caaggttatg tctgtgcctg ctgcagccgg cctgtggcgg tgcgcgagcc
      781 ggttcgctgg agggttcatc tccgccgggg gtgggtgcgg taatgccgca aatggaccaa
      841 atattttctg gaaccaaggc ctttcaggcg ccgatattgg cgcgcagcct cggacagctc
      901 gcgacgtcgt tcggcgggtt tttcgccacc tgcaccgcaa tgtacctgtg cctggccgtc
      961 tcgctttgga tcacgctgcc aatgtcggcg ctcgccgccg ggtttctcgt ccgcattttc
     1021 atcatccagc atgattgcgg acacggctcg ttcttcaggt cgcgatgggc aaacgagctg
     1081 atcggcagcc tatgcagcct aatgacgctg accccctatg ccttctggcg tcgccagcac
     1141 gccagacacc acggttcctg gaacaatctc gaccggcgcg cggcaagtgg gctggatatt
     1201 tactcgtcgt gcatgacggt cgcggaatac cgggccctcg gacgctggcg tagatgcctg
     1261 gtccgcctca ccaatcatcc gatcgtcgcc aacctgttgc tgccgccctt ggtgtttgtc
     1321 atcctctatc gagtgccgtt cgacgctgcc aagggatggc ggcacgagcg cctcggggtt
     1381 tacctgacca accttgcctt ggccgatttt atcggcggct tgggcctcgc tgtcggctat
     1441 ggccgtgtcg ccgccgtcca gctgccgatc atggtaatcg cctcgatcgt cggcgtctgg
     1501 ctgttttcga tccagcaccg ctttgagcat gcgcagtgga tgccggaggc aagctggagt
     1561 tttgccgcgg cctcgttcag aggtacttct tatcttcgcc tgccgcgccc gctccagtgg
     1621 tttaccggca atatcgggtt tcaccacgtg caccatgtag acccgcggat cccgaattat
     1681 cggctcgagg aatgccacaa cgccgatgcc gggtttcaat cggcgtcaac gcttactttg
     1741 cgcacggcgc tgcaggccct gcgctatgca ttgtgggaca gcgatctgga gcgcctggtg
     1801 ccgtttcgct tcgccgccgc cgattagacg atatctgcgg cataccaacc agcattcgag
     1861 gattgatatg gccagacgtt ccaccgttcg ctgcgtcgag gaatttattc tgttcgatgc
     1921 cctctacgag gacgagacgt cgtggcgatc acctccgcct tcctgcggac cagatcggcc
     1981 gccaattccg gtagccgatc gtaacggccc tccgcccagc ggaattcgat cgccaggttg
     2041 ccgccctcga catagcgttc ggaggggggt ggggggcatg gagaaatcgg aggaacaagc
     2101 cgatgcatcc agagggaggg gggctgagcg acccgcacgc tccgcacacc cgacgcgatc
     2161 cacaaggcgc tttgccggcg gcggatattg gcttctcccg tggccaatcc tcgcggtggt
     2221 agctgggcgc tcgcctctga taagcggcat tatcggcgct gccgctatgt ggtcgattca
     2281 atcgcctccc ctccctctgg atgcatgggc ttgttcctcc gatttctccg tgcccccacc
     2341 cccggtgtcg cgcctctatt gacccgcgac aggtttatct cagagagttc aattactcat
     2401 tccgagcgcg cagagcccgc ttggccgccg gccgtcgatt cgcccgctcc gaccaccgcg
     2461 ggcgaggaag gcgccatcgg gcggcagccg cgtgcgtagg ccaagatacc ttcgataccg
     2521 tctatcaatc ggctgttgag ctgaaccggc tgagggatat gtaaccaggg atccgtctat
     2581 gactggtatt tggtgg
//
LOCUS       DASWGM010000105         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_560949,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000105 DASWGM010000000
VERSION     DASWGM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560949"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1165
                     /locus_tag="VGA15_03160"
     CDS             245..1165
                     /locus_tag="VGA15_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9586727.1"
                     /translation="MLRPTIIAALGAILLAFPAAAQVPPFPAGFRTQEIATNGATLHV
                     RIGGKGPAVLLLHGYGETGDMWAPLAAELAPGHLVVVPDLRGIGLSSRPASGYDKKTQ
                     AGDIAGVLDALKIEKADLVTHDIGNMVGYAFAAQHPDRVTKFVLMDAPLPGIGPWDQI
                     VRSRALWHFSFGGPDAERLVAGRERIYLDRFWNEFSADPKKFDEASRQHYAKLYARPG
                     AMHAGFAQFAAFDQDALDNQALLAKGKLTMPVLAIGGEKSFGPTMAVVMRAAAADVRE
                     MVIPDSGHWLMEEQPKATVAAVRAFIDKGQ"
     gene            1292..>1758
                     /locus_tag="VGA15_03165"
     CDS             1292..>1758
                     /locus_tag="VGA15_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586728.1"
                     /translation="MILGMSIATFTTVHVILSLIGIASGIVVLFGMLGAKRLHGLTAI
                     FLATTVLTSVTGFLFPHDRLLPSHIVGIISLVALALAILALYVYGLAGSWRWIYVATA
                     VVALYLNVFVAVVQAFQKLPAVRSLAPTQSEPPFLIAQAVVLAIFVVLGGIAL"
BASE COUNT          311 a          568 c          532 g          347 t
ORIGIN      
        1 aagtcgtagg gtccataatc atgccagtaa tcgcagtgtg gataacactc gacttccgcc
       61 cgaacggcgc aaccaaagaa tacgaaggcg aagaggaccg ccgcaaagag tctgagcata
      121 tttggcaatc cctccgaagg acataagcgt tgcctatcac accgttattg ggggtacaat
      181 gattccggtg ggcgtttcac aaggggacgc tacgacatcc cgcgcgcaga cattggggga
      241 acacgtgctt cgaccgacaa tcatcgccgc actcggcgca atcctgcttg cgttccccgc
      301 cgctgcgcag gtgccgcctt tcccggccgg tttccgcacg caggagatcg cgaccaacgg
      361 cgcgaccctt cacgtgcgca tcgggggcaa ggggccggcc gtcctgttgc tccacggcta
      421 tggcgagacc ggcgacatgt gggcgccgct cgcggccgaa ctggcacccg ggcacctcgt
      481 cgtcgtcccg gacctgcgcg gcatcggcct ctcgtcgcgt ccggcgagcg gatacgacaa
      541 gaaaacccag gcgggcgata tcgccggcgt gctggacgcg ctcaagatcg aaaaggccga
      601 tctcgtcacc cacgatatcg gcaacatggt cggctatgcg ttcgccgccc aacatcccga
      661 tcgcgtcacc aagttcgtcc tcatggacgc gccgctgccc ggcatagggc catgggacca
      721 gatcgttcgc agccgtgctt tatggcactt ctcattcggt ggcccggatg ccgagcggct
      781 ggttgcaggt cgcgaacgca tctatctcga tcgcttctgg aacgaattct cggccgatcc
      841 gaagaagttc gacgaagcgt cgcggcaaca ttacgccaag ctgtacgccc ggcccggcgc
      901 catgcatgcc ggctttgcgc aattcgccgc gttcgatcag gacgcgctcg acaatcaggc
      961 attgcttgcc aagggcaagc tgacgatgcc ggtcctggcg atcggcgggg aaaagtcgtt
     1021 cgggccgacc atggccgtcg tcatgcgcgc cgctgccgcc gatgtccggg aaatggtgat
     1081 ccccgactcc ggtcattggc tgatggaaga gcagccgaaa gcgaccgtcg cggcggtgcg
     1141 cgcgttcatt gacaagggac aataggcgcg tcacgcaatt cgacctacgc cgaccctgtc
     1201 acgagatttt gcggctggtc cgccattccc cggcttgtgc gcgtcgaagc aatcccgcta
     1261 tcttcgaaga tccttggaga ggaggccggt catgattttg ggcatgtcca ttgccacctt
     1321 cacgacggta cacgtgatct tgagcctgat cggcatcgcc agcgggattg tcgtcctgtt
     1381 cggcatgctg ggcgcaaagc gcctccacgg cctgactgcg atcttcctgg cgaccacggt
     1441 gctgacgagc gtaaccggct tcttgtttcc gcacgatcgc ctcttgccgt cccatatcgt
     1501 cgggatcata tcgctggtcg cgctcgcgct tgcgatcctc gcgctctatg tctatggcct
     1561 ggcgggatcc tggcgctgga tctacgtggc gactgccgtc gtcgctctct atctcaacgt
     1621 cttcgtcgca gtcgtgcagg cgttccagaa gctccccgct gtaaggtccc tcgcacccac
     1681 gcagtcggag ccgccgttcc tcattgcgca agccgtcgtg ctggcgatct tcgtggtgct
     1741 cggcggcatt gcgttgaa
//
LOCUS       DASWGM010000106         8715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1392805,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000106 DASWGM010000000
VERSION     DASWGM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392805"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VGA15_03170"
     CDS             complement(<1..480)
                     /locus_tag="VGA15_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9586729.1"
                     /translation="MPTSARPKRRPARRPLPPPKPYHHGDLKRVLIDAALGLVKEGGA
                     EAVSVREAARRAGVSPGAPFRHFPSRAALLTAVAEEAQRRFRAEIEAALSEAPACDPL
                     ARFRSFGLAYLRWAMRNPAHFEIISSGRFFDHDKATGLSQDNAELIGMTERMLADAFA
                     "
     gene            592..1215
                     /locus_tag="VGA15_03175"
     CDS             592..1215
                     /locus_tag="VGA15_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586730.1"
                     /translation="MPSLRRNWFQGWRLFAVLTLVLIALSIWIAGMRQFEADGVRMVI
                     RFTARTSLLFFCLAFSAAALARLWPNAWTRWQRSNRRYLGVTFAASHAIHAVAIVCFA
                     AMDPAGYAAATSAASYIFGGIGYAFIFAMAATSFDRTTAAIGPRAWRILHLTGGYYLW
                     FQFMVAFGKWIPDMPLYALFLIPLLAVMALRLIAMASARSARTVRAG"
     gene            complement(1267..1707)
                     /locus_tag="VGA15_03180"
     CDS             complement(1267..1707)
                     /locus_tag="VGA15_03180"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEX9586731.1"
                     /translation="MREILRTLIAAASICLLTSAAHAGDQGDPAQSCDGNTNEMVECL
                     KAKTAQWDKRMTVAYQQALKDAVPQQHDQLRAAQRLWIQYRDANCLYYGEHFPAKWNP
                     VSRKKMLLNNVGAYSNRKTGCHFCGIRAARAPSPGSMPANACAA"
     gene            complement(1715..2566)
                     /locus_tag="VGA15_03185"
     CDS             complement(1715..2566)
                     /locus_tag="VGA15_03185"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9586732.1"
                     /translation="MQSVVITGASTGIGWATAKLLLDRGFRVFGSVRKQADADRLKSE
                     FGANFTPLLFDVTDEAAVLAAAREVRAALGGETLFGLVNNAGIAVAGPVLELAADEFR
                     RQMDVNVIGPIIATQAFGPLLGADSSLKGKPGRIVMISSVAGKNGNPLASAYSASKHA
                     IEGLSESLRREMMLFGIDVIIVAPGAVKTPIWSKADDVDVSVYKNSPFFPALEKVRQF
                     TKHLSEIGLPPEKIAEVIAEALTASSPKVRYQITPDPMRHLITAVLPKRMVDKIIAKR
                     LGLMPLT"
     gene            complement(3028..3429)
                     /locus_tag="VGA15_03190"
     CDS             complement(3028..3429)
                     /locus_tag="VGA15_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586733.1"
                     /translation="MAVFFTVAAVGVSGLLWFVLHDVVAEEWSESAHLLLTVHGVSAY
                     ALLVAVGSLLPLHVRSGWLRKRNVITGVMITAVMVALSATALMLYYGGEETQAVAKWL
                     HLAFGLGSFVLFPAHALLKVRTRRRAGMVVT"
     gene            complement(3457..4296)
                     /locus_tag="VGA15_03195"
     CDS             complement(3457..4296)
                     /locus_tag="VGA15_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX9586734.1"
                     /translation="MRRSRPLLGTFVEVEASGLAEPALERSVTRAFDAIERVHRLMSF
                     HDPESDISRLNRHAAFSPVTVDPWTAKVLHKARVLFEATDGLFDCAVGYEAMQRGLLP
                     SQDLHHVGRGTFSAVEFLADNRVRFSAQIALDLGGIAKGFAVDRAIAVLRAQGVGEAL
                     VNAGGDMRVMGGTAQPVYIRCPGANGHVVQGGLLHNAAIATSSATATQRKGNVQRLGP
                     PASATSSAEESAYSVVAPTCLLADALTKVLVQVGNSGTSYFAQFGATAFVTSGDVIEG
                     RAA"
     gene            complement(4293..4739)
                     /locus_tag="VGA15_03200"
     CDS             complement(4293..4739)
                     /locus_tag="VGA15_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX9586735.1"
                     /translation="MKASHWVWLPVAVISTSAYATTYFTVEQAQRAIFPGASFTSMGQ
                     PNTWRTSNGGYFVVERVVGKHEYITVAIGINPNGTVKQIEIMDYRESYGYEVRDASWR
                     AQFVGKSASSPLQLGADIKNISGATLSSKHITDGVKRVLQNLRAKG"
     gene            complement(4751..5788)
                     /locus_tag="VGA15_03205"
     CDS             complement(4751..5788)
                     /locus_tag="VGA15_03205"
                     /inference="COORDINATES: protein motif:HMM:NF042727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6662 family protein"
                     /protein_id="HEX9586736.1"
                     /translation="MGSKGVAMVGRICLRASRLSALRPRKKRGGGDASQTAKRIHARA
                     SRTGCAVALAAMLLMVGSAAFAEESMFAFVYTTDLLPQGGKEVEQWITWRHQKNGGTY
                     DQLEGRTEFEYGLASNLQAAVYLNYAWTQAYHNGPFGATTPPEQFADYVAGPDDFFNA
                     KRFTGVSGELIYRILSPYTDGIGLALYTEPTIGKNFFELENKVILQKNYLDDLLTIAV
                     NFTYAREFRNVPSNDNPPVFNWQEETDINFGLAVSYRFIENWSAGFEFLNEREFNSFD
                     FSHLSNSGYYLGPTIHFGGEHFFLTATALWQMPWATVHTDTVPGSLVNHLVLDNDFER
                     FRFRLKAGYTF"
     gene            complement(5905..6093)
                     /locus_tag="VGA15_03210"
     CDS             complement(5905..6093)
                     /locus_tag="VGA15_03210"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEX9586737.1"
                     /translation="MNARVNKSADDGKAVAAGPIGRSPRMVSSRDLLRGERLLIIKHE
                     EDIYRLQLTAAGKLILTK"
     gene            6944..8032
                     /locus_tag="VGA15_03215"
     CDS             6944..8032
                     /locus_tag="VGA15_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX9586738.1"
                     /translation="MQQAENSIIAELEDAVRNGSSEKRVGTLRQVTDLFLHDGDRLSD
                     DQVKVFDDVLCILIARVESRAKAELSQRLAPLDYAPFEVIQHLARDSDIAVAGAVLAN
                     SSRLRTSDLVEIASTRGQDHLLAISGRTDLPEAVTDVIVDRGERKVIRKLANNASARF
                     SDAGYSGIVAHAEADAELVEILGLRIDLPLRFLSDLLRRATEAVRARLMAVAPAELQE
                     EIKRVLKAISDAARGDKPARNFARAEQLVKLMKGLNELNDAAVIKFAETKKFDEVAAS
                     LALLNNVPTDMMARLLEGPRSDLVLIPCKSAGWSWLAVEAVLQHRPSRQKIDEQTLKV
                     AWRDYGKLSEETAQRTLRFWQVHNKLEK"
     gene            complement(8352..>8715)
                     /locus_tag="VGA15_03220"
     CDS             complement(8352..>8715)
                     /locus_tag="VGA15_03220"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX9586739.1"
                     /translation="APEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEYGEYVTG
                     PRIVTAETKAEMKRVLNDIQSGKFARDWMLENKVNQTSFKATRAKLAAHPIEEVGVKL
                     RDMMPWIKKGALVDKTKN"
BASE COUNT         1673 a         2695 c         2596 g         1751 t
ORIGIN      
        1 cgcgaaggcg tccgccagca tgcgctcggt catgccgatc agctcggcgt tgtcctgcga
       61 caacccggtc gccttgtcgt gatcgaaaaa cctgccgctc gagatgatct cgaaatgggc
      121 gggattgcgc atcgcccagc gcaaataggc aaggccaaag cttcgaaacc gcgccagcgg
      181 atcgcaggcc ggagcctccg acagcgccgc ttcgatctcg gcccggaagc gccgctgcgc
      241 ctcctcggcc accgccgtca gcagcgcggc gcggctcgga aaatgccgga acggcgcccc
      301 cggcgacacc ccggcgcggc gcgccgcctc gcgcacgctg acggcctcgg cgccgccctc
      361 cttgaccaat cccagcgccg cgtcgatcaa cacgcgcttg agatcgccgt gatgataggg
      421 ttttggcggc ggcaacgggc ggcgggcggg ccgacgtttc ggtctggcgg aggtgggcat
      481 gcgccgttct agcatcaacg aaacgtgaat gtaagcactg catacacgat tgacggccgg
      541 cgccggaggc tatgtaactg atgattacat cattcgcgaa aggagagcat catgccgtcg
      601 ctgcgccgaa actggttcca aggctggcga ctgttcgccg tgctgacgct ggtgctgatc
      661 gccttgtcga tctggatcgc aggcatgcgg cagttcgagg ctgacggcgt gcggatggtg
      721 atccgcttca cggcgcgaac ctcgctgttg ttcttctgcc tcgccttcag cgccgcggcg
      781 ctggcgcggc tttggccgaa cgcgtggacg cgatggcagc gcagcaaccg gcgctatctc
      841 ggcgtcacct tcgccgcgtc gcatgccatc catgccgttg caatcgtctg ctttgcagcc
      901 atggatcccg ccggctacgc ggcagcgaca tcggctgcct cctacatctt cggcggtatt
      961 ggctatgcct tcatcttcgc catggcggcg acgtcgttcg atcgcaccac agctgcgatc
     1021 ggcccgcgcg cctggcgcat cctgcatctc acgggcggct attatctgtg gtttcagttc
     1081 atggtcgctt tcggcaagtg gattcccgac atgccgcttt acgctctgtt cctgattccg
     1141 ctgctggcgg tgatggcgct gcggctgatc gcgatggcgt ccgcgcgatc cgcgcggaca
     1201 gtgcgggcgg gatagccgcc agctatttgt gaccgaggcc ttccaactcg cgggcgcgcg
     1261 cctcggtcat gctgcgcatg cattcgccgg catcgatccg ggcgatggtg ccctcgcagc
     1321 gcgtattccg caaaagtggc aaccggtttt gcgattagaa tacgcgccga cattatttag
     1381 gagcattttc ttgcggctaa ccggattcca cttagccgga aaatgctcgc cgtaatacag
     1441 gcaattggcg tcgcgatatt gaatccatag gcgctgcgcc gcgcgcaact ggtcgtgttg
     1501 ctgcggcacg gcgtccttga gggcctgctg gtaggcaacc gtcatccgct tgtcccattg
     1561 cgcggtcttg gccttgaggc attcgaccat ctcattggtg ttgccgtcgc aggattgggc
     1621 cggatcgccc tgatcgccgg catgggcggc tgatgtcaga aggcaaatcg aagcagccgc
     1681 gatcagcgtt ctcagtatct cgcgcatgtc catctcatgt cagcggcatc agcccgagac
     1741 gcttggcgat gatcttgtcg accattcgct tcggcagaac ggccgtgatc aggtgccgca
     1801 tcggatccgg cgtgatctgg tagcgcactt ttgggctcga cgccgtcagg gcctcggcga
     1861 tcacttccgc gatcttctcc ggcggcaaac cgatttcgct aaggtgcttt gtgaattgac
     1921 ggaccttttc gagcgccggg aaaaatggcg aattcttgta gaccgaaacg tcgacgtcat
     1981 cggccttgct ccagatcggg gtcttcaccg cgccaggtgc tacgatgatg acatcgatgc
     2041 cgaacagcat catttcccgg cgcaggcttt ccgacagccc ctcgatcgcg tgcttggagg
     2101 ccgaataggc cgaagccagc gggttgccgt tttttccagc caccgaactg atcatcacga
     2161 tccgccccgg ctttcctttc agcgaggaat cggccccgag cagcgggccg aacgcctggg
     2221 tagcgatgat cggtccgatc acgttgacgt ccatctggcg gcggaattcg tcggcggcga
     2281 gttcgagcac cggtcccgca acggcaatgc cggcattgtt gacgaggcca aacagcgtct
     2341 cgccgcccag cgccgcacgg acctcgcgcg cggcggccag caccgcggcc tcgtcggtga
     2401 catcgaacaa aagcggggtg aaattcgcgc cgaattcgct cttgaggcgg tcggcgtcag
     2461 cctgcttgcg cacgctgccg aatacacgga atccgcgatc gagcagcagt tttgcggtcg
     2521 cccagccgat gccggtcgac gccccggtaa tgacgacaga ttgcatttca gattccccct
     2581 cggctcgcag atcgttcccc gcgcctcttc tgctcgcaga gttaagcaga ccggaggcca
     2641 aagcaagttc gatgcgcgca ccagcggcga cgcgcttgct cgcctcgtgg ccatacgtgg
     2701 ctaaatttga agcgctttaa agattctcgt ttcaaccatt catcgcggcc atgcagattt
     2761 tcgcacttgc cctgatgctt tcgcgcaacg acctgtcgtc aatgaacagg gcaccgaacc
     2821 catcctaggg ttcggtggta tgtccgctct cgggggtcga ccggaagttg acggccgggt
     2881 gctggattgg ccccttccgc ctccatcgat tggcacaaga ctgatatttc gcagaagggg
     2941 tcgtgcttgc ggcctcccca gctcatcgca cggcgatatg gcctcgaaac cttatttgat
     3001 atttcgcgcg agcaacaact aatgttctta agtaacaacc atgcccgccc gccggcgcgt
     3061 tcttaccttc agaagcgcat gagctggaaa caagacgaat gaacctaaac cgaatgccaa
     3121 gtgcagccat ttggccaccg cttgtgtttc ttccccacca tagtacagca taagtgcagt
     3181 tgcgctcaga gcgaccatga ccgccgtgat catgacgcca gttataacgt tgcgcttacg
     3241 cagccatccc gatcggacgt gaagcggcag cagcgagccg acggccacca gcaacgcgta
     3301 agccgaaacg ccatgcaccg tcagcagaag gtgcgcggat tcgctccact cttcagccac
     3361 gacatcgtgc aaaacgaacc aaagcagacc cgatacgccc accgcggcga ccgtgaaaaa
     3421 aactgccacc ttctgccaaa atcctagacg catcgttcag gccgccctgc cttcgattac
     3481 atctccggaa gtgacaaatg ctgtggcgcc gaactgggca aagtagctgg tgccggaatt
     3541 cccgacctgt accagaactt tcgtcaaggc gtcggccagc aggcatgtcg gagcaaccac
     3601 cgagtaggcc gactcctccg ctgaggaggt ggccgacgcc ggcggcccca gcctttgcac
     3661 attccccttg cgctgggttg ctgtggcgga agaggttgcg atcgcggcgt tgtgcaagag
     3721 gccgccctgg acgacgtgac catttgcacc gggacagcgg atgtagacgg gctgggccgt
     3781 tccgcccatc acccgcatgt ccccgcccgc gttgaccaac gcctcgccca cgccctgcgc
     3841 gcgcaacacc gcgatggcgc ggtcaaccgc aaagcctttg gcgatgccgc caagatcgag
     3901 cgcgatctgg gctgaaaacc tgacccggtt gtcggccaaa aattcaaccg ccgagaacgt
     3961 tccacgcccg acatggtgga ggtcctgcga aggcagcaag ccgcgctgca tcgcctcata
     4021 gccgaccgcg caatcaaaca ggccgtcggt cgcttcaaac aggacccggg ctttgtgcag
     4081 gacttttgcc gtccagggat cgacggttac gggtgaaaat gcagcgtgac ggttgaggcg
     4141 cgatatatcg ctttcaggat cgtgaaagct catcagccga tgcacccgct cgatcgcgtc
     4201 gaacgcgcga gtgaccgaac gctccaaggc cggctccgca aggccgctgg cttcgacctc
     4261 gacgaaagtt ccaagcagcg gacgcgagcg cctcatccct tcgccctcag attttgcagg
     4321 actcgcttaa cgccatcggt gatgtgcttt gacgatagcg tcgcgccgct gatgttcttg
     4381 atgtcggcgc cgagctgcag cggcgagctc gcgctcttgc cgacgaactg cgctcgccat
     4441 gaggcgtcgc gcacctcgta gccataggac tccctgtagt ccatgatctc gatctgcttg
     4501 accgttccgt tcggattgat gccgatcgca accgtgatgt attcgtgctt gccgaccacc
     4561 cgctcaacga cgaaatagcc gccattggat gtccgccacg tgttcggctg ccccatgctg
     4621 gtgaaagacg cgcccggaaa aatcgcacgc tgtgcttgct caacggtgaa atacgtcgtt
     4681 gcataggccg aagtggagat cacggcgacc gggagccaaa cccaatgact tgctttcatc
     4741 gtgcaccacc tcagaacgtg taaccggcct tgagcctgaa gcggaagcgt tcaaaatcgt
     4801 tgtccagcac caggtggttc acgagagaac ccggtaccgt atcggtgtgc acagtcgccc
     4861 atggcatctg ccacagcgcc gtggcggtga ggaagaaatg ctcgccacca aaatgaatgg
     4921 tgggtccgag atagtaaccg ctgttcgaca gatggctgaa atcgaagctg ttgaactcac
     4981 gttcgttcag gaattcgaac cctgccgacc agttctcgat gaagcgataa gacaccgcga
     5041 ggccgaaatt gatgtccgtt tcctcctgcc agttgaatac aggaggattg tcattgctcg
     5101 gcacgttccg gaattcccgt gcgtaagtga agttgacggc aatggtcaac aaatcgtcca
     5161 ggtaattctt ttgcaggatg accttgtttt ccagctcgaa gaaattcttg ccaatcgtgg
     5221 gctcggtgta gagagccaga ccgatgccgt cggtataggg actgagaatc cggtagatca
     5281 gttcgcccga aacgccagtg aagcgctttg cgttaaagaa atcgtccgga ccggccacgt
     5341 agtcagcgaa ctgctcgggc ggggtcgtcg cgccgaaggg accattgtga taggcctgcg
     5401 tccaggcata attgaggtac actgcggctt gcaggttgct ggcgaggcca tactcgaact
     5461 ccgttcgccc ttcgagctga tcgtaggtcc cgccgttctt ctgatgccgc caggtgatcc
     5521 attgctcgac ttccttgccg ccctgcggca gcaggtcggt ggtgtagacg aaggcaaaca
     5581 tcgattcttc ggcgaaggcg gcagaaccga ccatcaagag catcgccgcg agtgcgacgg
     5641 cgcaacccgt tcggcttgcg cgggcgtgaa tccgtttcgc cgtctgggag gcatcgccgc
     5701 cacctcgttt ctttctcggt cgcaaagccg ataaccgaga cgcacgcaga caaattcttc
     5761 caaccattgc cacccccttt gagcccatgc cgagacttgt acagtggctg gcttcctccg
     5821 ctttcgcaga ggtggctggc taaaaacttg aattgcttgc acgccgagcg tgaactcccc
     5881 gttcttgctc gacgattctt cccgctattt tgtcagaatg agcttgcctg cggctgtcag
     5941 ttgcagccga tagatatcct cttcgtgctt gatgatcagc aacctttcgc ctcgtaagag
     6001 gtctcgactg ctgaccattc gcggcgaccg tccgatgggc cccgcagcga ctgctttgcc
     6061 atcgtcagct gatttgttga ctcttgcatt catttgagcg cgttctgcaa ctattctgcc
     6121 tgccccgcag ccgcagccgc agagctcgtt tcgcgtattc tgctgaaccc tcggtaagct
     6181 atcggctcct gcaaggatca agcgtcgata cgtaaatcat gggagttagg tttgagacaa
     6241 gatgacccag ttagaatcca tctagctgtt tggattagaa cgactccaaa gacacgttcc
     6301 tcattgagtg ctgcaagcgc gccgtaggca atcattttaa tctcggtggc acgactgact
     6361 gcggccaatc taccgactat ggcctcagca tctcgatcga tgcgcacgcg aggaagtcgg
     6421 ctccgagata gtctgtcccg taaatgatgc ggtggacagc gcaggcaatg cgtcggattc
     6481 tcatccgcac cgctggtcga gcgcactgtt tgcaacgggc gtctagcagt gcgtcggcgg
     6541 gctaccgatg caatgcgcgt tttcggatca gtgtgaagtg ttcggcgagc ggctcgcacc
     6601 gatagcggcg gctatcgccg cgattatctt cgcgcgctcg cccaacgcgt cgaagtcgac
     6661 gcgcaagaac ttcgcatcat ggggtcgaaa agcgaactgc tgcgcaccct cgtcgccgct
     6721 tcaagcgcaa aacggcgggt tttggcgtgc ccaattttgt tccgaagcgg cgcgcctccc
     6781 gagattcgaa gcaccggtga cgatgtgccg cggagccgcc ccgagcaggg cgcagccacc
     6841 cggtatttct acgggggtct aatttaacgc gcgcgtaggc ggcgctggtc attgtcgtga
     6901 tttgaactcg cgcaatgtcc ggcaggccgc cgaggggata gtggtgcaac aggcagaaaa
     6961 ttcaatcatt gccgaactcg aagacgccgt aaggaatggc tcgtcggaga agcgggtcgg
     7021 tacccttcga caggtgaccg atctattcct gcatgacggc gaccgcctga gcgacgatca
     7081 ggtcaaggtc tttgacgacg tgctctgcat tcttatcgcc cgcgtcgaaa gccgggcgaa
     7141 agccgaactc agccagcggc tggcgccgct cgactacgca ccgttcgaag tcattcagca
     7201 ccttgcccgg gacagcgaca tcgccgtggc cggcgccgtg ctggccaact ccagccgcct
     7261 gaggacaagc gacctcgtgg agattgccag cacaaggggc caggatcatc tgcttgcgat
     7321 ttcgggccga accgacctcc cggaagccgt caccgacgtc atcgtcgacc gcggcgaacg
     7381 caaggtcatc cgaaagcttg ccaacaacgc cagcgcgcgc ttttccgatg ccggctattc
     7441 cggcatcgtc gcgcatgcgg aggcagatgc tgaactcgtc gagattctcg ggcttcggat
     7501 cgatcttcct ctgagattcc tgagtgatct gttgcgacgc gcgaccgagg cggttcgcgc
     7561 ccgactgatg gccgtcgcac ctgccgagct ccaggaagag atcaagcggg ttctcaaagc
     7621 aatctccgac gcggcgcgag gcgataagcc ggcgcggaat ttcgcccgcg ccgaacaact
     7681 cgtgaagctg atgaaaggct tgaacgagct gaacgacgcg gccgtgatca agtttgccga
     7741 aacaaaaaag ttcgacgaag tagccgcgtc gctcgccctc ctcaacaatg tgccgaccga
     7801 tatgatggcg cgactgttgg agggaccccg atccgacctc gtcctgattc cctgcaaatc
     7861 ggcgggatgg agctggctgg cggtcgaagc ggtcctgcaa caccggccgt ccaggcagaa
     7921 aatcgatgaa caaaccctca aggtcgcctg gagggattac ggcaagctgt ccgaggaaac
     7981 cgcccagcgc accctgcgct tctggcaggt tcacaacaaa ctcgagaagt agggtttcgc
     8041 agccgcgatc cggcgcgccc gctccacggg cagcgcgctc cctcgcccgg cattgcggcg
     8101 agagggatgg atgggtctcg tgtcccggac gcggtgcagc gctttttgcg ctgctccgca
     8161 gagccgggac ccattggtcg tcaagcaaga tggaccccgg attgcagcgc accacgccgc
     8221 aagtcatagc gtgtcgaaga cgcgcgtaaa cgcgctgatg gcgctgcgca gcatccgggg
     8281 aacgcatccg tcgtccttgc tccaatcgtg aacacgctga tggcgctgcg cagcgtccgg
     8341 ggaacggcat cctagttctt cgtcttgtcg accagggcgc ccttcttgat ccacggcatc
     8401 atgtcgcgga gcttgacgcc gacttcctcg atcgggtggg cggcgagctt ggcgcgggtc
     8461 gccttgaacg aggtctggtt gaccttgttt tccagcatcc agtcgcgcgc gaatttgccg
     8521 gactggatgt cgttgagcac gcgcttcatt tccgccttgg tctccgcggt gacgatgcgc
     8581 gggccggtga cgtattcgcc gtattcggcg gtgttggaga tcgagtaatt catgttggcg
     8641 atgccgcctt catagatcag gtcgacgatc agcttcacct cgtgcaggca ttcgaagtag
     8701 gccatttccg gcgcg
//
LOCUS       DASWGM010000107         6784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_424734,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000107 DASWGM010000000
VERSION     DASWGM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424734"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1633..2145)
                     /locus_tag="VGA15_03225"
                     /pseudo
     CDS             complement(1633..2145)
                     /locus_tag="VGA15_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2532..4451
                     /locus_tag="VGA15_03230"
     CDS             2532..4451
                     /locus_tag="VGA15_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX9586740.1"
                     /translation="MKPLSRRRAISLASSFLATAPLLGSAVRAQPATGTGPAVFDMAN
                     FLGPGSDADAAFARAIAAISKAAGDAAKSGGPVHIVFNLGKNATYRINRPLAFKALHG
                     FELNGNGAQLINTTRGSTLTISGSSHVTIRDLTIDYDPLPFTQGTVAAFDKAALQISV
                     KVDAGYPDDPAFLATVTDGFFKVMDRRTKALKPGARDFLQPAKVERISPGLIKVHLRW
                     SANDRFPSQLPIAVGDTVTIANGYAHAIVVDGSAATSLIDLKLLASPGMGILENGGAG
                     SMMLQKVSVVPGPRPKGATMDRLISTNSDGSHFITVERGPTMEDCSFANTSDDAVNVH
                     GFYYYVVDKPAPRRYLLTPKWDIGLAAGDEIESCDHATFRSLGRSKIEQFTKRHAPEL
                     KGKIAPIWKNKSPTTQPDLIYDVVLQQDLALKAGDSVTSLSRIGAGTAIRRCMFHACG
                     RVLVKAPNAVVEDCQFNYSSANALQAGSDIGFWSESGFAENLVLRNNRFTHSITGANE
                     LTGGSGALGTIYIGMSFPEGAKGFQNNFQNRNVTIEGNHIDDSYIYAIFVSNADGVKI
                     IGNSIGQTFVRGTAYDAGQLYGIKPDSAILVGRSRNVEINNNTVARGKVAKTAVAVDG
                     TCDKGTVRVGNNRLT"
     gene            complement(4490..5440)
                     /locus_tag="VGA15_03235"
     CDS             complement(4490..5440)
                     /locus_tag="VGA15_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9586741.1"
                     /translation="MNAERSSPDHDRLAGLLAEAGELRPELHRYCARLMGSVIDGEDV
                     VQDTLVRAFVALEDMTKQGLEETPPLRPWLFRIAHNRALDLLRGRAVRMTEPIDAAAD
                     VVDQANPDPVEMLMRQEAVKTAVSRFAELPTLQRSVVILKDVLDEQLTEIAALLDLTV
                     DAVKGHLARGRARLRQINAQAGPLSDARPASAAVARYVALFNQRDWDGLRALLADDVK
                     LNQSTYPPRAGAAEVGMFFTIYAKFEGVWLAPAWLEGREVIAVFEDRADPEPSYFMWL
                     EWRDGRISFIRDYRYARYVVADAELKLAPDAKPGGDGVAH"
     gene            complement(5437..6195)
                     /locus_tag="VGA15_03240"
     CDS             complement(5437..6195)
                     /locus_tag="VGA15_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9586742.1"
                     /translation="MSLKDKNVVVTGGSRGLGLGLVEALVAHGAHVTVVARDADALGI
                     VRARLGVATISADVTDETAAHRILSQVRPDILALNAGAKPRMGRLDQLSWADFTATWE
                     TDVKAGLYWLQAALNLPLKPGSRVLVGSSGAAENGSPLSGGYAGAKRMLWLMAKYANG
                     VAEQKGLGIRFQAIVPQQIIGGTGVGDAGANAYARAANIKAEEFLARFGAPMPPREFG
                     EKVVSVLNDPKYAEGFAFGLKGDTGITMLEGAAA"
     gene            complement(6360..6665)
                     /locus_tag="VGA15_03245"
     CDS             complement(6360..6665)
                     /locus_tag="VGA15_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586743.1"
                     /translation="MSEAYCGGSASGAIRQENGHAPTLGAAGWLGLAAAPTFAIMALL
                     TGVGGGPLDMLCSAAQGASPLSGMVPMYLLMTAFHSAPWLKLISSRRKAAPAKGEKR"
BASE COUNT         1300 a         2177 c         2057 g         1250 t
ORIGIN      
        1 cgtcatgccc cgcgcatgga gtatgacggc cgtggtatcg ttcgcagaca tgatttttcg
       61 atcccgcggc gcgagacgcc cgggttgtgc ataactttgt ccctccttta gacagagggc
      121 gcagggaaag ccgggtgtcc gatgcacccg cagcctcgtg tgcgtagtgt agaaagcaca
      181 cgagtagtca ccacaggctc accggatcaa tccggctttc cctgcacaat ggttttaacg
      241 gcttatttcg tgctctcccc ggtgaccggg ctcttttgcc accgtcgcct cacggatatc
      301 cggcgcatcc caacccggtt gggcggctgc acctccgcaa gacttgacac cagcgtcggg
      361 gtgtcaggac cacacgactt cgccgtccgc gacctgcatc gttcgtctgc gtgctggccg
      421 atcgctcacg ggaaacccgc cctgcgatca cctctgcatg ccgacgctgt cgcgtccacc
      481 gcatcccgcc ccgcgtccgt gacgatcgcg agccgcccct ctgggagcga gacagttgga
      541 atcaataaag ctgtttctac caaaccgcga agcgaaatat ttttcgaatc ggggctggac
      601 actgatttac tgatttgccc gacgggctgt ttgcgagcga aggctgatgg gcgtccttag
      661 agtccgctca aaatgaaacc aataaaatca ggctatattg ccgtcattgc gaggagcgaa
      721 gcgacgaagc aatccataca ttcttttcgg ctcgatggat tgcttcgcgg agcccgtcat
      781 cgggcgcgca ttcgcgccat ggcatggcag ggcagaagaa gaataaaagc gaagcatcat
      841 tatcctttga ggtcactccg gcgcggcgtg aagcccctgc aggaatccgg gtcagcacct
      901 cgaagcagga cacagagaac cagcgtttgg ttggtattcg gcctcgatgc caaaagagct
      961 gaaccgaatt tccaggacga gctaccaacc gccgcggcgc cagaaatcct catcgtaagt
     1021 atgccgccgc tgccacgcgc ggcctgtgac ataaaagacg acgaccgtag caaccacaag
     1081 ggcgatgaca agaatgggaa ccgcatgatc cataggaacc tcccatgctg cgtgggaccg
     1141 cgttgtaaag gagtcgcagt gaaagtcaaa agggtaacaa tttccggccc atggcgttcc
     1201 tactggcggc ccgacggggg tcgggctttc gccctttaag tcattgatat tgatgcatcc
     1261 gaaatggatc gcctgaacgc atcagggcaa ttcagctcgc ggatgcttcg caagacgtgg
     1321 aaatgttcct tgccgaagct gcaagggcgg ctgggacagg tcggtgcgac gttatgtttg
     1381 gctgttgtgc ttatcgcttc agcgaacggc gaaggccgca atcttgggcg gcttcgacct
     1441 tgttacactc gcttctgaca gctacgggtc tgtaccgtgg gccgatgaaa ggttttacaa
     1501 aaatgcgatg aaatccttga tgaactctag aatcaacaag ggcatggcgt ggtcaggaac
     1561 cgattcctta ctgcacggcg ttaccattga gaaacctcga ttgagtcatt catctgagag
     1621 gtctagggac ttcgtcgcga cttcgagcgc cgcaaacagt gttgtggtgc catgccgctt
     1681 gtagtcgtgg ctctggccgg tcaaggcgcg gccattgggc aacttcagat agccctgcgc
     1741 tcgctccaaa gcctggatcg aaggcttttc gtccacgcat agcacaatcg ccttctcggg
     1801 cggggcgaca tagaggccaa caacatcggc ggcttttgct gtaaagttcg ggtcgttgct
     1861 ctcacaccag ggcttgcgag ccgcgaggtc aatcttgtgg ctgcgcagga accgccagac
     1921 atattggacg tcatggtcgg catagcgatg ccgccaaagg ctgacaatcc gcggctggac
     1981 cccaacttcc ttggctatcg agcgagtgct gcgcccggcc gccgccaaca gaactatccg
     2041 cgcccgcttc aaatcgcgct gcaatgtcac cggtgagcgg caacacgcct caagcacctt
     2101 gcgatctttc ctcgaaaggt ggacttctct tgcttcgggt atcatcccga tcttgaatca
     2161 cgactcacgc tctaaggaaa gtgggtacta ggcactgatt tgcccgacgg gcggctcgcg
     2221 ggcaggaacg aaaaatgcga tcgctcaaac tgagggagct tgttcagctt cgccggcgct
     2281 taccgaaaaa cgacccagcc gacgaaaacc aagcagatga cgactgcgcc taagaccggt
     2341 atgacgccct tgccctctag ttcaattggc ccgcgctgcg cccgccgatc aagccgatga
     2401 gcgcaccaac agccgaagcg cgcaatgtca aatctcggcg agttgccttc cgttccatga
     2461 cggttcatgt agcttggcct accatgccct ggtgcgatcg tcttgacgcg atcgctaaca
     2521 ggagccttta catgaagccc ctcagccggc ggcgggcgat ctcgctcgcc tcctcgttcc
     2581 tggcgacggc acccctgctg ggcagcgcag tccgggcgca gcctgcgacc ggcacaggac
     2641 cggccgtttt tgacatggcg aatttcctgg gccccggcag cgatgccgat gcggccttcg
     2701 ctagggcaat cgccgcgatt tccaaggcgg ccggtgatgc ggccaagtcc ggcgggccgg
     2761 ttcatatcgt cttcaatctc ggcaagaacg ccacctaccg gatcaatcgc cccctcgcct
     2821 tcaaagcgct tcacggtttc gagctcaatg gcaacggcgc acaattgatc aacaccacgc
     2881 ggggatcgac gctgacgatc tccggctcca gccatgtcac catccgcgat ctcaccatcg
     2941 actacgaccc gctgccgttc acgcaaggaa ccgtcgccgc attcgacaag gccgcgcttc
     3001 agatctccgt caaggtcgat gccggctatc ccgatgatcc cgcgttcctg gccacggtca
     3061 cggacggttt tttcaaggtc atggaccgcc gcaccaaggc gctgaaaccg ggcgcacggg
     3121 atttcctcca gcccgccaag gtcgaacgga tctcgccagg gctgatcaag gttcatttgc
     3181 gctggagcgc gaacgatcgc ttcccgagcc agctgccgat cgccgtcggc gataccgtca
     3241 cgatcgccaa cggctacgcc catgcgatcg tggtcgacgg cagcgccgcg acaagcctca
     3301 tcgacctgaa actcctggcc tcgcccggca tgggaatcct ggaaaacggc ggcgcgggct
     3361 cgatgatgct gcagaaggtg tccgtcgtgc ccggaccgcg gcccaagggt gcgacgatgg
     3421 accggctgat ctccaccaat tcggacggct cgcacttcat caccgtcgag cgcggaccga
     3481 cgatggagga ctgcagcttc gccaacacct ccgacgatgc cgtgaacgtg cacggcttct
     3541 actattacgt cgtcgacaag ccggcgccgc ggcgctattt gctcactccc aaatgggata
     3601 ttggcctcgc cgcgggcgac gagatcgaaa gctgcgatca tgcgaccttc cgctcgctgg
     3661 gaaggagcaa aatcgagcag ttcaccaagc ggcacgcccc cgaactcaag ggcaagatcg
     3721 cgccgatctg gaagaacaag agcccgacca cccagccgga tctgatctac gacgtcgtcc
     3781 tgcagcagga tcttgccctc aaggccgggg attccgttac ctcgctgagc cggatcggcg
     3841 ccggcaccgc gatccggcgc tgcatgtttc acgcctgcgg ccgcgtcctc gtcaaggcgc
     3901 caaacgcggt cgtcgaagac tgccagttca actattcctc ggcgaacgcg ctgcaggccg
     3961 gcagcgacat cggcttctgg tcggagtccg gctttgccga gaacctcgtg ctcagaaaca
     4021 accgtttcac ccacagcatt accggcgcca acgagctgac cggaggcagc ggcgcgctcg
     4081 gcacgatcta catcggcatg tcatttcccg aaggcgcgaa gggctttcaa aacaattttc
     4141 agaaccgcaa cgtgacgatc gagggcaacc acatcgacga ttcctacatc tacgcgattt
     4201 tcgtcagcaa cgccgacggc gtcaaaatca tcggcaacag catcggccag acttttgttc
     4261 gcggcaccgc atatgacgcc ggccagctct atggcatcaa gcccgacagc gcgatcctgg
     4321 tcggaaggtc gagaaatgtc gagatcaaca acaacacagt ggcacgggga aaggtggcaa
     4381 agaccgcagt cgccgtggac gggacctgcg acaagggtac ggtgcgtgtg gggaataaca
     4441 ggctgacgtg agtgggctgg acccgacgtc gattggccga ttcctcggtt cagtgcgcga
     4501 cgccgtcccc tccgggcttg gcgtccggcg ccagcttaag ctcggcgtcg gcgacgacat
     4561 agcgggcata gcggtagtcg cggatgaagc tgatccggcc gtcgcgccac tcgagccaca
     4621 tgaagtagct cggctccgga tcggcgcggt cttcgaagac cgcgatcact tcccggccct
     4681 cgagccaggc aggcgcaagc cacacgccct cgaacttggc gtagatggtg aagaacatgc
     4741 cgacctccgc ggcgccggcg cgcggcgggt atgtggactg attgagcttg acgtcgtcgg
     4801 ccagcagtgc ccgcaggccg tcccagtccc gctggttgaa cagcgcgaca tagcgcgcca
     4861 ccgcggcgga cgccggccgc gcgtccgaaa gcgggccggc ttgcgcgttg atctggcgaa
     4921 gacgcgcgcg accccgcgcc agatggccct tcacggcgtc gaccgtgagg tcgaggagag
     4981 cggcgatctc cgtcagctgt tcgtcgagca cgtccttcag gatgacgacg ctgcgctgca
     5041 gggtgggaag ctccgcaaag cgcgacaccg cggtcttgac cgcttcctgg cgcatcagca
     5101 tctccacggg gtcagggttg gcctggtcga cgacatcagc ggcggcgtcg atcggctcag
     5161 tcatgcgcac cgcccgaccg cgcagcaggt ccagcgcgcg gttgtgggcg atccggaata
     5221 gccagggtcg aagcggcggc gtctcctcca ggccttgttt ggtcatgtcc tccaacgcca
     5281 cgaaagcccg gaccagcgtg tcctgcacga cgtcctcgcc gtcgatgacc gatcccatga
     5341 gacgcgcgca atagcggtgc agttcgggcc gcagctcgcc ggcctcggcg agcagccccg
     5401 ccaggcggtc gtggtctggc gaggaacgtt ctgcgttcaa gccgccgctc cctcgagcat
     5461 tgttattccc gtgtcgccct tgagcccgaa ggcgaagcct tcagcatatt tcgggtcgtt
     5521 caatacggaa acgactttct cgccgaactc ccggggcggc atcggcgcgc cgaagcgtgc
     5581 caggaattct tcggccttga tgtttgcggc gcgcgcgtag gcgttggcgc cggcgtcgcc
     5641 gaccccggtg ccgccgatga tctgctgcgg cacgatcgcc tggaagcgga tgccaaggcc
     5701 cttctgctcg gcgaccccgt tggcgtactt ggccatgagc cagagcatgc gcttggcgcc
     5761 ggcatagccg cccgatagcg gcgatccgtt ctcggccgcg ccgctcgatc ccaccaggac
     5821 gcggcttccg ggcttgagcg gcaggttgag cgccgcctgc agccaataga gcccggcctt
     5881 gacgtcggtc tcccaggtcg cggtgaaatc cgcccagctc aactggtcca gccgacccat
     5941 ccgcggcttc gcgccggcgt tcagcgccag gatgtccggg cggacctggc taaggatgcg
     6001 gtgggcggcg gtctcatccg tcacgtcggc ggagatcgtg gcgacgccca gccgggcgcg
     6061 aacgataccg agcgcgtcgg cgtcgcgggc gaccaccgtc acgtgtgcgc cgtgcgcgac
     6121 cagcgcctcg accaaaccca ggccaaggcc acggcttccg ccggtgacga cgacattttt
     6181 gtccttgagg ctcatcagtt ctgctccagt gtctgggtgt ccagctgtag ctaagacgat
     6241 cgaggctcgg aaaaggagtc agtcaacgcc gcgggggctc gccttggcat acataaactt
     6301 ccctttcttg aggccaccgg ccgcaagcca ctcttcacat gttccggtct tgtggtcggt
     6361 catcgtttct ctcctttggc cggtgcggct tttcgtcggc tggagatcag cttcagccaa
     6421 ggcgccgaat ggaaggcagt catcagcaag tacatcggga ccattccgct cagcggtgac
     6481 gcaccttgcg cggccgagca gagcatatcc agcgggccgc cacccacgcc cgtaagcagc
     6541 gccatgatgg cgaaggtcgg cgcggccgcg aggcctagcc agccggctgc gccgagggtg
     6601 ggcgcgtggc cgttctcctg acggatcgcg ccactggcgg atccgccgca ataagcctcg
     6661 ctcatgcttt ctctcctgca agtaaggcgt gggtgggcca gcggcgtcag gcgctcctcc
     6721 accggcgcca catcggcgat cgcgttcgtc agcgtgctaa ttttcgcggt tccggaacgc
     6781 cgcc
//
LOCUS       DASWGM010000108         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2642071,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000108 DASWGM010000000
VERSION     DASWGM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2642071"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..543
                     /locus_tag="VGA15_03250"
     CDS             58..543
                     /locus_tag="VGA15_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9586744.1"
                     /translation="MTRGPSRRMRAADAKLAALKATGTLNRNPHAVVDPLFRGHGFFD
                     PRDLLQVRYEMVRRHDVEEVPMAMTAQLFGVSLPTAYQAQAAFAAEGLAGLLPKRRGP
                     KQGHKLTPEILAYIEQRRSERPAWRISDLQADLQHTFGLTIHRRSLERVLRGKKKLTS
                     R"
     gene            814..2898
                     /locus_tag="VGA15_03255"
     CDS             814..2898
                     /locus_tag="VGA15_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9586745.1"
                     /translation="MGDSRVASHHLSRDAYLYIRQSTLRQVNENGESTRRQYALRDRA
                     IAIGWPAQRVHIIDQDLGMSGAHSVARDGFQELVAAVGLRKVGIVLGLEVSRLARNSV
                     DWQQLLQLCAYTDTLILDEEGIYDPSAFNDRLLLGLKGTMSEAELHLLRNRLRGGILN
                     KARRGELALRLPVGLVRLADGRCARDPDAGIQASVAAVFESFAATGTIASAVRQLLAS
                     GIRFPQLVWGGNKAGTIIWCDYNRTRVINILTNPAYAGAYAYGRRRCRQAPDGHIERR
                     KLKPDAWAVVIPEHFTGYVSWTEFDAIQRQLRSNAQAFGRPDAFGPPREGPALLQGRV
                     MCGRCGSAMYVRYGGIHGHQPRARYVCVDQADARRAACQSVPASDVDAAAARLGLELM
                     TPMAIEMTLAIQDELDRRVAQREQHHQLRVTRARYESDFARRRFMLVDPANRLVAAGL
                     EAEWNTRLAELATVEEELARFRAETQQQLSVDMQNRIRSLSRDLPQLWADPAVIDRER
                     KEILALLIQDVTILSEHTEITAHLRLRGGACHTLTVTRSSIAPRKRTPPDIVAKIDQL
                     LEIGDDAIVAQELNAAGIRNWRNGPFTKGQITNVRKGRTLRAHHDRRHAGGYATAGEL
                     AARYNVTRTTIRHWAITGLLERFSCGKRHRWYYRVPAGADILRGYGGPYAKPPQIVAA
                     STCNSCEPGAVR"
BASE COUNT          576 a          971 c          961 g          538 t
ORIGIN      
        1 gatattgatt cgctgaaggc gtcgaacagc gacagaattg accgaggagc ggcgtaattg
       61 accagaggac cgtccagacg catgcgtgcg gcggatgcca aacttgcggc gctcaaggcg
      121 accggcacac tcaaccgcaa tccgcacgcg gtcgtcgatc cgttgttccg cgggcacggc
      181 ttcttcgacc cgcgcgactt gctgcaggtg cgctacgaga tggtgcgccg gcatgatgtc
      241 gaggaagtgc cgatggccat gacggcgcaa ctgttcggtg tgtcgctgcc gacggcgtat
      301 caggcacagg ccgccttcgc ggcggaaggg ctcgccggtt tgctgccgaa gcgtcgcggg
      361 ccgaagcaag ggcataagct gacgccggag atccttgcct atatcgagca acggcggagc
      421 gagcgccccg cctggcgcat cagcgatctg caggccgatc tgcagcacac cttcgggctc
      481 accatccatc gccgcagcct cgagcgggtg ctccggggca aaaaaaaatt gacgagccgc
      541 tgaggccatc gccatgccgc cgagacgcca tcgactacga gcggctgcgc agcaaggcgc
      601 tgcgcggcga tcccgcccaa aactccacgc tcctgcctcg cggtcttgct gcgtggctca
      661 gtgccgcgtt gctgatggtc ggcccccgta tcgtcagcgc atcgccatcg tcacacatcg
      721 cgacggagct gccattgact gagatgtcga gccgcggacc gctgccagcg gcattggcat
      781 cgatcatcct gcgtatgacg aaggaggcta tcgatgggtg attcgcgtgt tgcttcccac
      841 catctgagcc gtgatgccta tctttacatt cgccagtcga cgttgcggca ggtcaatgag
      901 aacggcgaga gcacgcggcg gcaatacgcg ctgcgcgacc gcgccatcgc gatcggctgg
      961 ccggcgcagc gcgttcatat catagatcag gatcttggga tgtccggagc ccacagcgtg
     1021 gcgcgcgatg gcttccaaga actggttgcg gctgtcggtt tgcgcaaggt tggcatcgtt
     1081 ctcgggctag aggtctcgcg gctggctcgc aactcggtcg actggcagca actgctgcag
     1141 ctgtgtgcct acaccgatac gttgatcctt gacgaggagg gcatctacga cccatcggca
     1201 ttcaacgacc gtttgttgct cgggctcaaa ggcacaatga gcgaggccga gctgcatttg
     1261 ttgcgcaatc ggttgcgcgg cggcatcctc aacaaggcgc ggcgcggcga actcgccctg
     1321 cgtttgccgg tcggtctggt gcgcttggcc gatgggcgtt gcgcgcgcga ccccgatgcc
     1381 ggcatccaag ccagcgttgc ggcggtattt gaatccttcg ccgcgacagg cacgattgcc
     1441 agcgcggtgc gacaactgtt ggcctccggt atccgctttc cccagctggt ctggggtggc
     1501 aacaaggcag gcacgatcat ctggtgcgat tacaatcgca ccagagtgat aaacattctg
     1561 accaacccgg cttatgccgg cgcctatgca tatggacgcc gacgctgccg ccaagcgccg
     1621 gacgggcaca tcgagcgtcg caagctcaag cccgatgcat gggccgtggt gatccccgaa
     1681 cacttcacag gctacgtgtc gtggaccgag ttcgacgcca tccaaaggca gctgcgcagc
     1741 aatgcgcaag cctttggccg ccccgacgcg ttcggtccgc cgcgagaagg gccggcgctg
     1801 ttgcagggcc gcgtgatgtg cgggcgatgt ggcagcgcga tgtatgtccg ttacggcggc
     1861 attcatggtc accagccgcg agcgcgttat gtctgcgtgg atcaggccga cgcccgccgc
     1921 gccgcgtgcc agagcgtgcc tgcatctgat gtcgatgccg cagccgcccg tctgggcctc
     1981 gagctcatga cgccgatggc catcgagatg acgctggcaa tccaggacga actggatcgc
     2041 cgggtcgcgc aacgcgaaca acatcatcaa ttgcgggtca cccgcgcgcg ttacgagagc
     2101 gacttcgccc gccgccgctt catgttggtt gatccggcca atcgtttggt cgccgcaggc
     2161 ctcgaagcgg agtggaatac acgcctagcc gagttggcga cggtcgagga agagctcgca
     2221 cgcttccgtg ccgagaccca acagcaactg agcgtcgaca tgcaaaatcg catccgttcg
     2281 ctcagtcgcg acctgccaca gctctgggct gatccggccg tcatcgaccg cgagcgcaag
     2341 gagatccttg cacttctcat ccaagatgtg accatcctga gcgagcatac cgagatcaca
     2401 gcccacctgc gactgcgggg tggtgcttgt cacaccctga cggtgacacg atcctcgatt
     2461 gcgccacgca agcggacccc acccgacatc gttgcaaaga tcgaccaact gctggagatc
     2521 ggggacgacg cgattgtcgc ccaagagctc aatgccgcgg gcatccgaaa ctggcgcaac
     2581 ggccccttca ccaagggcca gatcaccaac gttcgaaagg gtcgcaccct gcgcgcccat
     2641 catgaccgtc gccacgctgg cggctatgcg acagccggag aactggccgc gcgctacaac
     2701 gtcacccgaa caacgatccg tcactgggca ataactgggc tgctcgagcg gttctcatgc
     2761 ggcaaacgac atcgctggta ctaccgcgtt ccggctggag cagatatcct cagaggatac
     2821 ggcggccctt atgccaagcc gccgcagatc gtagcggcgt ccacctgcaa ctcgtgcgaa
     2881 ccaggagcag tccgatgagc acaccaagat gtgcgcttcg acgcggtgct caagctggtg
     2941 gtagatcggg cggagttgca ggtcgtcctt gagattcttg aacgcggcct cgacttcgac
     3001 gagctggata tagaactgcc acaggtgggc cggctctcgg tcgcac
//
LOCUS       DASWGM010000109         7778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2226685,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000109 DASWGM010000000
VERSION     DASWGM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2226685"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VGA15_03260"
     CDS             <1..1225
                     /locus_tag="VGA15_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9586746.1"
                     /translation="DSPDRLGCGFEDDMQQASGGLADRNYFRALADNATATIGWLQTH
                     GVDFTIPVYYLSAGPARIQPVGGGSAIVEKLLDAAKRAGVKVRYESRVTRLVMAEGHR
                     VCGIEFQCSDGVTTMLDADAANLASGGFQGNAAMMRAQFGLGAETLRLISPGTGFDTG
                     DGIRMAMDQGARVSGDWNGMHIEPVDPRAKNSAPVVLVYPYGIVVDQDGRRFFDEGCG
                     LVHETWEVFARDIHFGRPKNIAYAILDSRLFDIPGHERAIRSEVPPYQSEVLEDLAAQ
                     IKIPAGNLRETVDTFNAAATLDVARFDATRCDGLAAADTLNPPKSNWARAITQPPYLA
                     YPLVGAIAYTFGGLATNERAEVLSEQGPMPGLYAAGETTGHFYRTAPNAVSVLRALVF
                     GRVAGREAVSFLQTR"
     gene            1258..1644
                     /locus_tag="VGA15_03265"
     CDS             1258..1644
                     /locus_tag="VGA15_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9586747.1"
                     /translation="MASENNGAFIRNIAEVPWREFPNHFGGALSKPLVMPETAGSRHL
                     DYRISMYQPMAHVARHKHQVQEQIYHVLEGEGLMEIAGKNHVVRKHDFIFLPPGVEHA
                     ISNSGLVDLVFLVITSPVTDDGKNVV"
     gene            1667..3439
                     /locus_tag="VGA15_03270"
     CDS             1667..3439
                     /locus_tag="VGA15_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9586748.1"
                     /translation="MVPRVMSASDAYDVVVIGAGAGGMTAAAIAAAEGLSVLVIEKTE
                     FVGGTTSWSGGMVWIPVNSKIKQAGLDDSLSDAADYLASTVPEIENADLRDTFLKRGP
                     EAVEYLEANTEVRLQPVKTYPDYYPEKPGATVGGRVLEPVSFDGARLGANFGRLRPPL
                     PEFTLFGGMMVNRLDIPHLRKFGRSFRSTLRSMRLVSQYALQRLRAPRGTTLHLGNAL
                     AARLYASLLARNIEIWFGTSVEHLLVEDDAVRGVGIAVSSGSRPIVARKGVVLATGGF
                     SHDQNFRGRYFPSAAGAVSAASPAGTGDGLRIAIAAGASVGTHVASPAYWVPASRFQR
                     ADGSAGVFPHTVTDRAKPGVIAVNRYGNRFVNEALSYHEFVLAMLRDGNDAAGRSFYL
                     VCDRHFLWAYGLGRIRPFTLRLKRYVRSGELIEAPGIDALADLIGVERSALSATVDKY
                     NAHAQVGLDPEFGRGTTIYQRHLGDAGHRPNPCVAPIEQPPFYAMRIYPADLGTAVGL
                     RTDCHARVLRNNRTAITGLYACGNDMGSIMNGNYPGPGITLGPALTFGYIAGRHLAQT
                     YSEPRAQKNGHEERQEERQERGPA"
     gene            3436..4263
                     /locus_tag="VGA15_03275"
     CDS             3436..4263
                     /locus_tag="VGA15_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX9586749.1"
                     /translation="MKNPAADFGVNTYSYIFDGGAADTVARLADQGYGGVELMFFPGH
                     LWPAELDASGLRGLRDLCEQRLRLVAVNMPNVDMNIAAAAAEMRGYTLDLLIKFVRCA
                     GELGAGGIIVGPGKANPLFPMPRDRMISYFYRALDVLAPLARQVGTRLFIENMPFAFL
                     PDAESLMNIVDGYGDDSIRVIYDVANAHFIGEAPIDGLRRVRDRLSLVHFSDTTRQSY
                     KHDPLGCGDVPLAGIASVMKEIGYTELPMLEVISLKPDPDIADSCRRLQQAGFGGDA"
     gene            complement(4368..5675)
                     /locus_tag="VGA15_03280"
     CDS             complement(4368..5675)
                     /locus_tag="VGA15_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9586750.1"
                     /translation="MDRTLERSTMRKVYLRLLPFAVLSYVLAYIDRINVSFAGLTMRG
                     DLGMSAGTFGFAVGMFYWGYFIFEVPSNVILEKVGARLWIARIMITWGILAGLTAMVP
                     GSTSFAIVRFLLGVAEAGFFPGIILYFTYWFPSRHHARIVSGFLVGLPVAVAVGAPIS
                     TGLLGLDGLFGLRGWQVMYIAEAIPTVVIGVITFFVLTDRPEQAKFLTAEERSWLVAT
                     IAAERRATEAVRKFTLWQTLYNPKVLLLALNYLGIVTASLGMLIFIPQMIKSLGDYSN
                     MTVGWLTMIPYTCGAIAMVVWGRISDRMNERRWNLFIGCVFSTVGLIIAGMTMGTWWA
                     LVGMSIAAMGFYGSKGPFFAMPPMFLSGTGLAAGIAWINSIGNLGGFFGPWYVGVMKD
                     LTGNYAGGLYGLALLGLIAAIVCALFLHIPNRMPSSAVGAPAE"
     gene            5709..6125
                     /locus_tag="VGA15_03285"
     CDS             5709..6125
                     /locus_tag="VGA15_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586751.1"
                     /translation="MPFLKLRGQKGSLQFKNHWNRAVQNMGQLCCHTKLPSASPGSMP
                     VAIARAFDDQTLCPNERAVGFLSLQEISFGRKIGRLKKPKTGGATCKVRFRSTRRCRG
                     LGGLARAGLANNKLNLSLSREISGVILMQVLPFADK"
     gene            6295..>7778
                     /locus_tag="VGA15_03290"
     CDS             6295..>7778
                     /locus_tag="VGA15_03290"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9586752.1"
                     /translation="MIGAGFSGLHMLKSLRDKLGLKVLVYETGGTVGGTWYWNRYPGA
                     RCDSDAYIYCFTWDKQLLQEWEWSERYPEQHEILRYLEHVAKRHDLKRDIQFNTSVTG
                     AEFDERTNLWTVRTSTGEEVTARYLIAAVGTLSTTNMPQLKGLEKFKGKWYHTSRYPH
                     TGVDFTGRRVGVIGTGATGVQAIPEIAQQAKQLTVFQRTANYCVPARNGKVDPEVVKA
                     RKADYDGVAKRIRESFFGQEHYFLPKSALEATAEEREREFDRMWDAGGFAFWLANYQD
                     MFFSQQANDLCADYIKRKIRKTVKDPVIAEKLIPKGYAYGTKRQPLDTNYYETFNKEN
                     VRLVDAKTDGGIEEITETGIRAGDKEYPLDIIVFATGFDAMTGPLKALNLKGRGGRTL
                     DREWLDGPHTYLGISVAGFPNLFTITGPQSPSVLSNMPVSIEQHVEWVTDCIEAMRKT
                     GKTTIEATPAAQDQWVAHVNEIVNATLMTSANSWYMSANIPGKPR"
BASE COUNT         1629 a         2332 c         2328 g         1489 t
ORIGIN      
        1 cgattcaccc gatcggctcg gatgcggttt tgaagacgat atgcagcaag cgtcgggcgg
       61 attggccgat cgaaactatt ttcgcgcgct ggcggacaat gcgacggcaa caatcggttg
      121 gcttcagacc cacggcgtcg acttcaccat tcccgtctat tatctcagcg ccggcccggc
      181 ccggattcag ccggttggcg gcggcagcgc tattgttgaa aagcttctcg acgccgcaaa
      241 aagggcaggg gtgaaggttc gctacgaatc ccgcgtgacg cggctcgtga tggcggaggg
      301 gcatcgcgtc tgcggcatag agtttcagtg cagtgacggt gtcacaacaa tgctcgacgc
      361 cgatgccgcc aatctcgctt cgggtggatt ccagggcaac gctgcgatga tgcgcgcgca
      421 gtttggcctg ggcgccgaga ccctaagact catttctccc ggcacgggct tcgacaccgg
      481 cgatggcatc cgcatggcca tggatcaggg cgcgcgcgtt tcgggcgact ggaacggcat
      541 gcatatcgaa ccggtcgacc cgcgagccaa gaattccgcg ccggtcgtgc tggtgtatcc
      601 ttacgggatc gtcgtcgatc aggacggccg tcggttcttc gacgaaggct gtggtctcgt
      661 gcacgagacc tgggaagttt tcgcccgcga tatccatttc ggaagaccga agaatatcgc
      721 ctatgcgatc ctggactccc gcctgttcga tattcccggt cacgagcgcg cgatcagatc
      781 ggaagtgccg ccctatcaat ccgaagtgct tgaggatctt gcggcccaga tcaaaattcc
      841 cgcgggcaat ctgagagaga cggtcgatac gttcaatgcg gccgcaaccc ttgacgtcgc
      901 gcggttcgat gcgacgcgct gcgatggcct ggcggcggcc gacacattga atccgccaaa
      961 atcgaactgg gcgcgggcga tcacgcagcc gccgtatctc gcttacccct tggtcggcgc
     1021 gatcgcctat acatttggcg gtcttgccac caacgaaagg gcggaagtct tgtccgagca
     1081 agggccgatg cccggccttt acgcggcggg agaaaccacc ggccatttct atcgcaccgc
     1141 accgaatgcg gtttcggtgc tgcgtgcgct ggtttttgga cgagtggcgg gacgagaagc
     1201 tgtcagtttc ctgcagaccc ggtaacatct gaaacgatct tgggaggaac aacaaccatg
     1261 gcatcagaaa ataacggcgc tttcatccgc aacatcgccg aagtgccctg gcgcgaattt
     1321 cccaatcatt ttggcggcgc gctatcaaag cctctggtga tgccggagac ggcgggctcg
     1381 cgtcacctcg attaccggat ttcgatgtat cagccgatgg cgcatgtcgc ccgccacaag
     1441 catcaggtcc aggagcagat ctatcacgtg cttgagggcg aggggctgat ggagatcgcg
     1501 ggcaagaacc atgtcgtgcg caagcacgat tttatctttc tgccgcccgg agtcgaacac
     1561 gccatctcga attcgggtct ggtcgatctg gtgtttctgg tgatcacgtc gccggtcacc
     1621 gacgatggca agaacgtcgt ctgatctttc ttgcattctt gaccgcatgg tcccaagggt
     1681 gatgtcggct tccgacgctt acgatgtcgt ggtcattggc gccggcgccg gtgggatgac
     1741 ggctgccgcg atcgctgccg ctgaaggact gagcgtcctt gtgatcgaaa agaccgaatt
     1801 tgtcgggggt acgacgtcgt ggtccggcgg catggtctgg attcccgtca acagcaagat
     1861 caagcaggcc ggcctggatg atagtctatc cgatgccgcg gactatctcg ccagcaccgt
     1921 tcctgaaatc gaaaacgccg acctgcggga cacatttctc aagcgaggcc cggaggcggt
     1981 cgaatatctt gaagccaata ccgaggttcg gcttcagccc gtaaaaacct atcctgatta
     2041 ttatccggag aagccgggcg ccaccgtcgg cggccgcgtg ctggagcccg tcagcttcga
     2101 tggcgcgagg ctcggagcaa acttcgggcg gctgcgtccg ccgctgccgg aattcacctt
     2161 gttcggcggg atgatggtca accggctcga catcccgcac ctgcgcaaat tcgggcgctc
     2221 gtttcgctcg acgctacgtt cgatgcgtct ggtttcgcaa tatgcgctgc agcgtcttcg
     2281 cgcaccgagg ggcacgacgc tgcatctggg caatgcgctg gcggcgcgcc tgtacgcctc
     2341 gctgctggcg cgaaacatcg agatttggtt tggcaccagc gtcgaacatc tgttggtcga
     2401 agacgatgcc gtgcgaggtg tagggatcgc agtctcatcc ggcagtcggc cgatcgtggc
     2461 gcgcaagggc gtcgtgctgg cgaccggcgg cttctcccat gatcaaaatt ttcgcgggcg
     2521 ttactttccc agcgccgccg gagctgtttc cgccgcgtct ccagccggaa ctggcgacgg
     2581 attgcggatc gccatcgcag ccggcgcgag tgtcggtacg cacgttgcga gtcccgctta
     2641 ttgggtgccg gcgtcgcgtt ttcaacgcgc ggatggcagc gcgggcgttt tcccgcatac
     2701 cgtgaccgac cgggcgaagc ccggcgtcat tgcggtcaat cgttatggaa accgctttgt
     2761 caacgaagcc ttgtcctacc acgagtttgt cctcgccatg ctgcgcgacg gcaacgatgc
     2821 ggcgggccgt tcgttctacc tcgtctgcga tcggcatttt ctctgggcct atgggcttgg
     2881 ccgtatcagg cccttcacgc tgcgattgaa gcgatacgtc agaagcggcg aattgatcga
     2941 ggctcccggc atcgacgcgc tggctgatct gatcggtgtc gaaagatcgg cgctctcggc
     3001 aacggtcgac aaatacaacg cgcatgccca agtggggctc gacccggaat tcggccgcgg
     3061 caccacgatc tatcagcgtc atctcggcga cgccggccat cggcctaatc catgcgtcgc
     3121 gccgatcgag cagcctccgt tttacgcgat gcggatctat ccggcggacc tcggcacggc
     3181 ggtcggcctc cgaaccgatt gccatgcccg ggttctgcgc aacaatagaa ctgccattac
     3241 cggcctttat gcctgcggca acgatatggg ctcgatcatg aacggaaatt atccggggcc
     3301 cggcatcacg cttgggccgg ccctgacatt cggctacatc gccggccgcc atcttgcaca
     3361 gacctactca gagccgcgtg cccaaaaaaa cggacacgaa gagcgacaag aagagcgaca
     3421 ggagagggga ccagcatgaa aaatccagcc gctgactttg gcgtcaatac ctactcttac
     3481 atattcgatg gcggcgccgc cgataccgtc gctcggctcg ccgaccaggg ttatggcggg
     3541 gtcgagctga tgttctttcc cggccatctc tggccggccg aactcgatgc ctccgggctt
     3601 cgcggcttgc gcgatctctg cgaacaacgg ctgcggctgg tcgccgtcaa catgccgaat
     3661 gtcgacatga atatcgccgc ggccgccgcg gaaatgcgcg gctataccct cgatttgctg
     3721 ataaaattcg tccgctgtgc cggtgagctc ggagcaggcg ggatcattgt cggtcccggc
     3781 aaggctaatc cgctgtttcc gatgccgcgc gatcgcatga tctcgtattt ttatcgcgcg
     3841 ctcgatgtgc tggccccgct ggcgcggcag gttgggacaa gacttttcat cgagaacatg
     3901 ccgtttgcct tcctgcccga tgcggagtcg ctcatgaaca ttgtcgatgg ttatggcgac
     3961 gacagcattc gcgtgatcta cgacgtggcg aacgcgcact tcatcggcga agcccccatc
     4021 gacggattgc ggcgcgtccg cgaccggctc agcctggttc atttttccga taccacccgg
     4081 cagagttaca agcacgatcc gttggggtgc ggagatgtcc cgctggcggg cattgcatcg
     4141 gtcatgaaag agatcggata taccgagctg ccgatgctgg aagtcatttc gctcaagccg
     4201 gatcccgaca tcgccgacag ctgccggcgg ctccagcaag cggggtttgg cggcgatgcc
     4261 tgatcaggtc gcacttgtca tcgggccaat cccccctgag cggacatgcc aaagacgatg
     4321 aaaatgaccc aacccgggca ttgatttccg cgtgcgcgtg atcaggatca ttcagcaggc
     4381 gcgccgaccg ccgacgacgg catgcgattt ggaatgtgca gaaacagcgc gcaaacaatg
     4441 gcggcgatta gaccgagcaa agcgagccca taaagtccac ccgcgtagtt gccggtcagg
     4501 tctttcatga cgccgacata ccaaggtccg aagaaccccc cgagattgcc gatcgagttg
     4561 atccaggcaa tgcctgcagc gagtcccgtg ccactgagaa acatcggcgg cattgcgaaa
     4621 aacgggccct tggagccata aaaacccatc gcagcgatcg acatccccac aagcgcccac
     4681 caggtcccca tcgtcatgcc ggcgatgatc agtcctacgg ttgagaacac gcaaccgatg
     4741 aacagattcc aacgccgctc gttcatgcga tcggaaatgc gtccccacac caccatggcg
     4801 atagcgccgc aggtataggg gatcatggtg agccagccga ccgtcatgtt gctgtagtca
     4861 cccagcgact tgatcatctg cggaatgaaa atcagcatgc ccaggctggc ggtgacaatg
     4921 ccgagatagt tcaacgccag gagcagcacc ttgggattgt agagagtctg ccacagcgtg
     4981 aatttgcgca ccgcctcggt ggcgcggcgt tcggcggcga tcgtggcgac gagccagctg
     5041 cgttcttccg cggtcaggaa ttttgcctgc tccggccgat ccgtgagtac gaaaaatgtg
     5101 atcacgccga tcaccacggt cggaatcgct tcggcgatgt acatgacctg ccagccgcgc
     5161 agaccaaaca agccgtccag gccgagaagc cccgtcgata tcggcgcgcc gaccgccacc
     5221 gcaaccggta gaccgaccag aaagcctgaa acgatgcggg cgtgatgacg gctcggaaac
     5281 cagtaggtga agtagaggat gatgccagga aagaatccgg cctcagcgac gcccaacagg
     5341 aaacgcacga tcgcgaagct cgtcgaaccc ggaaccatcg cggtgaggcc cgccaggatc
     5401 ccccaggtga tcatgatccg ggcgatccat agtctggcgc cgaccttttc caggatcacg
     5461 ttgctcggga cctcgaagat gaaatagccc cagtaaaaca tgccgacggc aaacccgaag
     5521 gtgccggctg acataccgag gtcgccgcgc atggtgagac ctgcgaagct cacattgatc
     5581 cgatcaatat aggcgagcac ataagacaag accgcaaacg gaagcagccg cagatagacc
     5641 ttgcgcatgg tcgatcgctc gagcgtacgg tccattgctt cccccccatt tcatggcgag
     5701 ccgagcttat gccgtttttg aaattgaggg gtcagaaagg ctcccttcaa ttcaaaaatc
     5761 attggaatcg tgcggtgcaa aacatgggac agctttgctg tcacaccaaa cttccgtcgg
     5821 cctcacccgg atcaatgccc gtcgcaatcg cacgggcttt tgacgatcaa acgctttgcc
     5881 cgaacgaacg cgcggtcggt ttcttgtcct tgcaggaaat ctcgttcggg aggaagatcg
     5941 gccgtcttaa aaaaccaaaa acgggaggtg cgacatgcaa agtaagattc agatcgacca
     6001 ggcgttgccg cggcctcggc ggcctcgctc gagcaggcct cgccaacaat aaactgaatc
     6061 tctccctttc gcgcgaaatt tcaggtgtca tcctgatgca ggtgctgccg ttcgcggaca
     6121 aataaatgtc tgtgagaggt tcgcggctct cgaacgcggc gtctatgccg cagccgacgc
     6181 ggcgctgctg cggagtttct cgcgtttgcg gaggatcgag tttctctgtc gtcgatttaa
     6241 acaacaaaac gggaggaaca aatggctagc gcaattcaaa accttgacgt tgtcgtgatc
     6301 ggcgcaggct tttccggcct gcacatgctg aagtcgctac gggacaaatt gggattgaag
     6361 gtgctggtct acgaaactgg tggaaccgta ggcggcacct ggtactggaa tcgctacccc
     6421 ggcgcgcgct gcgattccga cgcctacatt tattgcttca cctgggacaa gcagctcctg
     6481 caggagtggg aatggtccga acgctacccc gagcagcacg aaatcctgcg ttatctcgag
     6541 catgttgcca aacgccacga tctcaaacgc gacattcaat tcaataccag cgtgacaggc
     6601 gccgaattcg acgagagaac caacctgtgg acggtgcgta ccagcacggg ggaagaggtt
     6661 acggcacgtt acctgatcgc ggccgtaggt actttgtcga ccacaaacat gcctcagttg
     6721 aagggtttgg agaaattcaa gggcaagtgg taccacacca gccgctaccc tcacactggc
     6781 gttgacttca ctggcaggcg cgtgggcgtg atcggcaccg gcgccacggg ggtgcaggcc
     6841 attcccgaaa tcgcgcagca agccaaacaa ctcaccgtgt tccagcgcac cgccaactac
     6901 tgcgtaccgg cgcgcaatgg caaggtggac cccgaagtgg tcaaggcgcg aaaggcggac
     6961 tatgacggcg tggccaagcg gatccgggaa tcgttcttcg gccaggagca ttacttcctg
     7021 ccgaagtcgg cgctggaggc cacagccgag gagcgagagc gtgagttcga caggatgtgg
     7081 gacgccggcg ggttcgcctt ttggctggcc aactatcagg acatgttctt ttcgcagcag
     7141 gccaatgacc tgtgcgccga ctacatcaag cgcaagatcc gcaaaaccgt gaaagatccg
     7201 gtgatcgccg aaaagctgat tcccaagggc tacgcctacg gcaccaagcg ccagccgctg
     7261 gacaccaact actacgagac cttcaacaag gaaaacgtac ggctggtcga tgcgaaaacc
     7321 gacggcggga ttgaagaaat caccgaaacg ggcatccgcg ccggtgacaa ggaatatcct
     7381 ctcgacatca tcgttttcgc caccggtttc gatgccatga ccggtccgct gaaggccttg
     7441 aacctgaaag gccgcggcgg gcgcacgctg gatcgggaat ggttggacgg cccgcacacc
     7501 taccttggca tctcggtcgc cggctttccc aacctgttca ccatcaccgg cccgcaaagc
     7561 ccgtcggtgc tgtcgaacat gccggtatcg attgagcagc atgtcgagtg ggtcaccgac
     7621 tgcatcgaag ctatgcgcaa gaccggaaag accaccatcg aggcgacgcc cgcggcgcag
     7681 gatcaatggg tcgcccatgt caacgaaatc gtcaacgcga cgctgatgac cagcgccaac
     7741 tcctggtaca tgagcgccaa catccccggc aagccgcg
//
LOCUS       DASWGM010000110         6090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1531490,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000110 DASWGM010000000
VERSION     DASWGM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531490"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VGA15_03295"
     CDS             <1..208
                     /locus_tag="VGA15_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586753.1"
                     /translation="ANAEAFAKGVERLEINVWDLPYALSPFKYQVKCLDLIRGKFASL
                     DAEHRTALRPVLERTGCWEHLVAR"
     gene            complement(242..871)
                     /locus_tag="VGA15_03300"
     CDS             complement(242..871)
                     /locus_tag="VGA15_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX9586754.1"
                     /translation="MTRPRVRIYTDYKSPYAFVANKRLFELEENHGVELEWLPYTLRI
                     PEFMGTVEERTPHFWRKVRYAYMDARRYANAQGLTMKGPRRIYDAFYSSAGMLFAQRH
                     GLFRPYHDTVFRRFWSHDLEIDELSGISGVIAAVGGSATDFEAYVNGPARAEHDRIID
                     EAEALGVFGVPTMVFNGEPFWGGDRIDMLIERIKKPETIAAALGSRHRA"
     gene            complement(938..1198)
                     /locus_tag="VGA15_03305"
     CDS             complement(938..1198)
                     /locus_tag="VGA15_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586755.1"
                     /translation="MTMKRSLLCLGLLMVLVIASGGRGLTQGAKTTPSFYIVLNSLTK
                     RCSVFDKTPKTDTPNITVASDAIYNTRADAEAAMNKLPSCNR"
     gene            complement(1831..3741)
                     /locus_tag="VGA15_03310"
     CDS             complement(1831..3741)
                     /locus_tag="VGA15_03310"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9586756.1"
                     /translation="MNIEVPRKQLDLSSAIAEGDIRVLLMVLVHMTGDERWLQPRYKP
                     ERDVRLIPDPEAGVPKEIQDEIRTAVLKLFVDGEPRPAITDPGDELMLKMMRACLGEN
                     VAPEYAPLMREEMGFIPRDARWTRRPSGDALMKQHVVIVGAGVCAIALGVALGRLGIP
                     YTIVEKNDELGGTWYVNRYPGCGVDTPNHSYSFSFGARNNWTRYFAQREELLDYLKKV
                     ALEHDVRSHIRFNTRLTSSRWDETRRRWISTLETGNGVEDFESTVLVSAIGQLNDPSP
                     AHFKGEEDFSGLTFHSALWSDDIKLEGQRVAVIGTGATAMQLVPSIADRVASVTVYQR
                     SAQWSRPVAGYSDPISEGAQWLLAHLPFYVQWYRFNMFWRYGDGLLPFLRKDPNWPYP
                     DRAVNKGNDRHRQELTDFILTELKDRPDLIEKCVPTYPPYGKRILLDNGWFRTLTKPH
                     VELVTEPIDHFVRDGIVTSDGKLRPADVIVISTGFKVTEMAARLNISGNAGKKLADAW
                     ADDNPTAYLGLTVPDFPNFFCMLGPNAGPAHGGSVIFQSECQSRYITACLVNMIEQGI
                     AAIDVRQDIHDRYVREVDAEHEQMIWTHPGMTTYYRNKQGRVFSAMPWRFVDYWRMTH
                     DADLSQYRLTKA"
     gene            complement(3753..4526)
                     /locus_tag="VGA15_03315"
     CDS             complement(3753..4526)
                     /locus_tag="VGA15_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9586757.1"
                     /translation="MIAEGIVLVTGGSRGIGAATATLLAQQGRRVVIADIAPEALIGT
                     QTILWPDGFDVASETGVISGIAEIETAHGPITGLVNAAGVFGKMHSVERVRMDNWDRE
                     INIDLRGTFLVARSVGVRMAKRRHGAIVNVASVAGMTSGPIHAYTAAKAGVIQITQTL
                     AAEWGRAGVRVNAVSPGFTRTAALEAGIASGALDQNRLARNTAMNRLVEPTEVAQAIA
                     WLLSPASSGVTGINLPVDAGFVAGSTWDAYGGLREAPEV"
     gene            complement(4516..5175)
                     /locus_tag="VGA15_03320"
     CDS             complement(4516..5175)
                     /locus_tag="VGA15_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9586758.1"
                     /translation="MTQVPEAAPGKLTKLNRVQRNAWTKRKIFDAATKIVGKYGYAEA
                     SVARITEEAGVAQGTFYNHFENRQELLDQLLPMIGTEMVRFIRERTGTAHAARQEIER
                     FGAFFDFIREVPEFLRILNEAEFFAPIGYQKHLDNISTAYVRILRRARHAGAIENFSD
                     EEFEAIVHMFMGARGYLSRRYSYSGGAVTAVPDHVISAYEKLITRGLFTPRSEGDKHD
                     R"
     gene            complement(5172..>6090)
                     /locus_tag="VGA15_03325"
     CDS             complement(5172..>6090)
                     /locus_tag="VGA15_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9586759.1"
                     /translation="VAGPLYHVGALDLPGIAVLWQGGMLSILRDFDAAQWLAAIEADR
                     LDGAWLAPVMTTSILTLPDRGRYDVSSLRWAIGGGEKTPEARIRAFSQYFTNARYIDA
                     YGLTETCGGDTFMEPGLEITKIGSTGRAVAHVEIEIRDDMGHRVAPGENGEICLRGPK
                     VTRGYWKDPEKTSAAFFGDWFRSGDIGYLDEDGFLYLTDRKKDMIISGGENIASSEVE
                     RVIYELPQVREVAVIGMPDQRWGERPIAVVVLADSAVLELPDLTEHCRQRLASFKVPK
                     QLIIRDSLPRNPSGKVLKRVLRAELEANA"
BASE COUNT         1153 a         1811 c         1869 g         1257 t
ORIGIN      
        1 cgccaatgcg gaagcctttg cgaaaggcgt cgagcgactg gagatcaatg tctgggacct
       61 gccttatgcg ttgtcgccgt tcaagtatca ggtcaaatgc ctcgatctga tccgcggcaa
      121 gtttgcctcc ctcgacgctg agcatcgaac ggcgctgcgg cccgttctgg agcgcacagg
      181 gtgctgggaa catctcgttg caaggtagcc gatgatatca gtcgtggcgg caacggccgt
      241 ttcatgctcg gtgccggctt cccagcgcgg ccgcgatggt ttcgggcttt ttgatgcgtt
      301 cgatcagcat gtcgatgcgg tcgccgcccc agaacggttc gccgttgaac accatggtgg
      361 gcacgccgaa cacgccgagg gcttccgcct cgtcgatgat gcggtcatgt tcagcgcgcg
      421 ccggaccgtt gacgtaggct tcgaaatccg tcgctgagcc gccgacggca gcgatcacac
      481 cggaaatgcc ggacaattcg tcgatttcga ggtcgtgact ccagaagcgc ctgaataccg
      541 tgtcgtggta agggcggaac agcccgtggc gttgcgcgaa cagcatgccg gcgctggaat
      601 agaaggcatc gtaaattcgc cgcggccctt tcatggtaag cccttgcgcg ttggcatagc
      661 gccgcgcgtc catataggcg tagcgcacct tgcgccagaa atgcggcgtg cgctcctcca
      721 ccgttcccat aaattcaggg atgcgaaggg tgtagggcag ccattcgagt tcgacgccgt
      781 ggttttcttc cagctcgaac agccgcttgt tggcgacgaa ggcgtagggg cttttgtagt
      841 cggtatagat tcgtacgcgt ggtcttgtca ttgccggtcc tcgtctagat tgggcaattc
      901 ttcccgatcc agacgaggga tgcaacccgc agcggtttca tcgattgcag gaaggcagct
      961 tgttcatggc ggcttctgcg tcggcccggg tgttgtaaat cgcatcgctc gccacggtga
     1021 tgtttggcgt gtcggtcttc ggcgtcttgt cgaagaccga gcagcgcttg gtcagtgaat
     1081 tcaacacaat gtaaaaggat ggagttgtct tcgcgccctg ggtgagcccc cgaccgcccg
     1141 aggcaatcac caagaccatc agcaagccga ggcagagcaa acttcgtttc atcgtcacct
     1201 ctcatgactc aaaggaggtt atcgcgcagt tgcgatttcc gctccggata gatcgatcca
     1261 aaaacggtcg ccggttacga acccggaact gccgatacat tgatccaatt gaatgggccc
     1321 cggtgcattg gagcatcgcg gggcccgaca tcttcgtcat tgcgaaggat ttctggtaca
     1381 caccagttcg ctctcaagat caaaaagcgc gtggcctttg cgcttcgatc ctgcggatat
     1441 gttcagattg ttggcggcgc aacgaggcta gcagctgccg ttcacctcaa cgtgcgccga
     1501 gaccttgcgg ttgagcaagg tcactggctt gtgccccggt ggcgtcatgc tcctctccaa
     1561 gagtggtccg acggacatgt gtccgcatcc gctgcctgtc gttcgatcac atgattggac
     1621 ggcaataccc gcgaagcgtg gagccaaatg ctccgctacc ccgacaggtg cagcaccaga
     1681 aatgctgcgg cagatagggg caggtgtcaa gttgcgtccg gcgaaccagc gcttgagttc
     1741 cggtgcttgg ccctacctgc aatggtaaac tggtgcagtt ccgccgcccg ctggggcggc
     1801 tccgcttcaa atcccacacc tggagccgcg tcaggccttg gtcaggcgat actggctcaa
     1861 gtccgcatca tgtgtcattc gccaatagtc cacgaaccgc cagggcatcg cggaaaatac
     1921 ccggccctgc ttgtttcgat agtaagtcgt catccccgga tgggtccata tcatttgttc
     1981 atgctcggcg tcgacctcgc gaacgtaccg gtcatggatg tcctggcgaa cgtcgatggc
     2041 ggctatgcct tgctcaatca tgttgacaag acacgccgtg atgtaacgtg actggcattc
     2101 ggactggaag atcacgctgc cgccatgcgc cgggccggcg ttggggccga gcatgcagaa
     2161 gaaattcggg aaatccggca ccgtgagtcc aagataggcg gtcggattgt cgtcggccca
     2221 cgcatcggcg agcttcttgc cggcgttccc gctgatattg aggcgtgccg ccatttccgt
     2281 gaccttgaat ccggtcgaga tcacgatgac atccgccggc cgcaacttgc cgtcggaagt
     2341 gacgatgcca tcgcgcacga agtgatcgat gggttcggtg accagttcga catgcggctt
     2401 cgtcagcgta cgaaaccagc cattatcgag caggatgcgc ttgccatagg gcggataggt
     2461 cggaacgcat ttttcgatca aatcgggccg gtctttcagt tcggttagga taaaatccgt
     2521 cagctcctgg cgatgccggt cattgccctt gttgacggcg cgatcgggat acggccagtt
     2581 cggatccttg cgcaggaaag gcagtagccc gtcgccgtaa cgccagaaca tgttgaagcg
     2641 ataccattgc acgtagaacg gcaggtgcgc gagcagccat tgcgcgcctt cgctgatggg
     2701 atcggaatag cccgcgaccg ggcgcgacca ttgcgccgac ctttgataca ccgtgacgga
     2761 agccacgcga tcggcaatgg aaggcacgag ctgcatcgcg gtggcgccgg tgccgatcac
     2821 agccacgcgc tggccttcca gcttgatatc gtccgaccac agcgcggagt gaaacgtcaa
     2881 gccggaaaaa tcttcttcgc ctttgaaatg agccggtgat gggtcgttga gctggccgat
     2941 tgcgctgacc aggaccgtcg actcgaaatc ttccacgcca ttgccggttt ccagcgtcga
     3001 aatccagcga cgtctggtct cgtcccagcg cgacgatgtc aaccgagtgt tgaaacggat
     3061 gtggctgcgg acgtcatgtt caagcgcgac cttcttgaga tagtccagca gttcttcgcg
     3121 ctgtgcgaaa tagcgggtcc agttgttgcg cgcgccgaag gaaaatgagt aggaatgatt
     3181 gggcgtgtcg acgccgcagc cgggataccg gttgacgtac caggtgccgc cgagttcgtc
     3241 gtttttctcg acgatggtgt agggaatgcc gagccggccg agcgcgacgc caagcgcgat
     3301 ggcgcagacg cccgcgccga cgatcacgac gtgttgcttc atcaaggcgt cgccggacgg
     3361 acgtctggtc cagcgtgcgt cgcgcggaat gaatcccatt tcctcacgca tgagcggcgc
     3421 gtattccggt gcgacgttct cgccgaggca cgcgcgcatc atcttcagca tcaactcgtc
     3481 gccagggtcg gtgattgcag gcctgggctc accatccacg aagagtttga ggacggcggt
     3541 gcggatttca tcctggattt ctttgggcac cccggcttcc ggatccggga tcaggcggac
     3601 gtcgcgctcg ggcttgtagc gaggctgtag ccagcgctca tcgccggtca tgtggaccag
     3661 gaccatcaat agaacgcgga tatcgccctc ggcgatggcc gaagacagat cgagttgctt
     3721 gcgcggcact tcaatgttca tgatcttccg tcctacactt ccggtgcttc gcgcaatccg
     3781 ccataggcgt cccaggtcga gccggcgaca aatccagcgt caactggtaa attgattccg
     3841 gtcacgccgc tgctcgcggg cgagagcagc cacgcgatgg cttgggcaac ctctgtcggt
     3901 tccaccagcc ggttcatcgc ggtgttgcgc gcgaggcggt tctgatccag cgcgcctgaa
     3961 gctattcccg cctcgagtgc cgccgtgcgc gtaaatccag gtgatacggc gttgacccgc
     4021 acgccggcgc gtccccattc cgccgcgagc gtctgcgtta tctgaatgac gccggccttc
     4081 gccgcggtat aggcgtggat cgggccggat gtcattcccg cgaccgaggc gacgttaacg
     4141 atggcgccat ggcgtcgttt ggccattcgc acgccgacgc tgcgcgccac cagaaacgtg
     4201 cctcgcaaat cgatattgat ctcgcgatcc caattgtcca ttcgcacgcg ttcgaccgag
     4261 tgcatcttgc cgaacacgcc ggccgcattg acaaggccag tgatcggacc gtgagcggtc
     4321 tcgatctccg ctatgccgct aatgacgccg gtctcgctgg caacgtcgaa cccatcaggc
     4381 cacaggatcg tctgcgtccc gatgagagct tcgggcgcga tatcggcaat aacgacgcgc
     4441 cgtccctgct gcgcgagaag ggtcgcggtt gcggcaccga tgccgcggct gccaccggtc
     4501 accagaacaa tgccttcagc gatcatgctt gtcgccttcg ctacgcggag tgaacaatcc
     4561 tctggtaatc agcttctcgt aggccgagat cacatgatcc ggcaccgcgg tgacggcgcc
     4621 gccggaatag gaataacggc ggctgaggta gccgcgagcg cccatgaaca tgtgcacgat
     4681 ggcctcgaat tcctcgtcgc tgaaattctc tatcgcgccg gcatgccgcg cgcgccgcag
     4741 gattcggaca taggccgtcg agatattgtc gaggtgcttc tggtagccga tcggtgcaaa
     4801 aaactcagcc tcgttgagga tgcgcaggaa ttccggcacc tcacggatga aatcgaagaa
     4861 agcgccgaat cgctcgattt cctgccgcgc cgcgtgcgcc gtaccggtcc gctcgcggat
     4921 gaaacgtacc atttccgtgc cgatcatggg caacaattga tccagcaact cctgacgatt
     4981 ctcaaaatga ttgtaaaacg tgccctgcgc aacgccggcc tcctcggtga tgcgggcgac
     5041 ggaggcttcg gcgtagccgt atttgccgac aattttggtc gcggcgtcga aaatcttgcg
     5101 cttggtccat gcattgcgct ggacgcgatt gagttttgtc agctttccag gtgcagcttc
     5161 gggtacttgc gtcatgcgtt ggcctctaat tcggcgcgca gcacgcgctt gagtaccttg
     5221 cccgagggat tgcgtggcag gctgtcgcga atgatcagtt gtttcggcac cttgaatgac
     5281 gccagacgct ggcggcaatg ctccgtcagg tcaggtagtt ccagcaccgc gctgtccgcc
     5341 agcaccacga ccgcaatggg ccgttcgccc caacgttggt cgggcatgcc gatcacggcg
     5401 acttcgcgca cctgtggcag ttcgtagatg acgcgctcga cttccgacga tgcgatattt
     5461 tcgccgccgg aaatgatcat gtccttcttg cggtcggtga gataaaggaa gccgtcctca
     5521 tcgagatagc cgatgtcccc gctacgaaac cagtcaccga aaaacgccgc cgaggttttc
     5581 tcggggtcct tccaatagcc gcgcgtgacc tttgggccgc gcaggcagat ttcgccgttc
     5641 tcgccggggg cgacgcgatg gcccatatcg tcgcggattt cgatttcgac atgggcgacg
     5701 gcgcggccgg tcgatccgat ctttgtaatt tccagcccgg gctccatgaa agtgtcgccg
     5761 ccgcaggttt cggtcaggcc gtaggcgtcg atgtagcggg cattggtgaa atattgcgag
     5821 aaggcgcgaa tgcgcgcctc gggagttttt tcaccgccgc cgatcgccca tcgcaggctg
     5881 gagacgtcat agcgtccgcg atcggggaga gtgaggatgg acgtcgtcat gacgggcgcc
     5941 agccatgctc cgtcgagccg gtcggcttca atcgcagcca gccactgcgc ggcgtcgaaa
     6001 tcacgcagaa tagagagcat tccgccctgc cacagcaccg cgataccggg aagatcgagt
     6061 gcgccgacat gatagagcgg gccggcgacc
//
LOCUS       DASWGM010000111         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1393222,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000111 DASWGM010000000
VERSION     DASWGM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393222"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VGA15_03330"
     CDS             <1..333
                     /locus_tag="VGA15_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018069623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9586760.1"
                     /translation="QGMDRNLVEAGQIDGATRWQNLRYIVIPLLYPTIRLSAFFAIVG
                     SLQLFDLIMPLTRGGPADSSHTMVSFLYTFGVMRMRVGFGSAVGVILFCICVTFAFTY
                     KRWFMRDE"
     gene            326..1177
                     /locus_tag="VGA15_03335"
     CDS             326..1177
                     /locus_tag="VGA15_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9586761.1"
                     /translation="MSDQATFSDRGFDLAALYKALFLTVVVVFVATPLVATVLGGFKS
                     LGELRTNPFGLPQVWEWENYGGILRSKRYWQLLGNSLIISTMTVVLTLIAASMAAFVF
                     AQIKFFGSAMLMSYITMGLLFPAATAILPLFIKVRDLGLLDSYFGVVLPQVAFALAMS
                     ILLLRRFFKDIPYELLEAARIDGCSYIEFFRYVTLPLSRPILATVGTIVFVNSWNSYL
                     IPLIMLNSDDLYPWPLGIMVYQGEYSSEWHLILAFVTLTILPTIILFLFAQKHIVAGL
                     TAGAVKG"
     gene            1222..2319
                     /locus_tag="VGA15_03340"
     CDS             1222..2319
                     /locus_tag="VGA15_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9586762.1"
                     /translation="MRKVGIGVIGCGVISKAYLTAMKQFSNIELRAVADMRSAAAEQR
                     AKEFDVPGMRVDQMLKRDDVEIVVNLTVPLAHTDVSLAVLNAGKHVHSEKPLGVNVAE
                     ARKVMDLAAQKNLRVGCAPDTFLGGGHQTARKLIDDGAIGTPVGGTAFFMCPGHERWH
                     PAPGFYYLRGGGPMLDMGPYYITDLVQLLGPIASVMGAATKPRLERLITSEPLNGTMI
                     PVEVATHVTGTLEFVSGAVIAITMSFDVPKHSHLPIEIYGTAGSLLVPDPNRFGGEVS
                     LAKTGGEWQPQPHTHGNTEGEFRSIGVADMAAAIVGNRPHRASGALALHVLEAMEAFQ
                     ISSDQGRRIKLETTVERPAMIPPGLATGQIN"
     gene            2336..2977
                     /locus_tag="VGA15_03345"
     CDS             2336..2977
                     /locus_tag="VGA15_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX9586763.1"
                     /translation="MREAMIVWGGWPGHDPDLCASMIRGWLKAEGFGVRIETTTEAFL
                     DPALKNLSLIIPIYTMSKIEKPEALALCDAVKSGVGLAGHHGGMCDAFRDNVDYQFMC
                     GGQWVAHPGNIIDYKVDVTRPDDPVMAGIDSFEHRSEQYYMHVDPAIEVLATTTFNAG
                     HAPWVDGVVMPVVWKKRFGQGRVFYSSLGHRAYELDVPEIRTLMTRGMLWAAR"
     gene            2974..>3365
                     /locus_tag="VGA15_03350"
     CDS             2974..>3365
                     /locus_tag="VGA15_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9586764.1"
                     /translation="MSADGERIPGRRATLEDVAREAGVSLATADRVVNQREGVRGQTI
                     ARVELAIAKLGYRANAAAMRLARNESFRFAFILPSNANSFMSNLSVQVERTSDWLASQ
                     NGYIDVMHVDVFDPNALASALERLSPSY"
BASE COUNT          619 a         1063 c         1010 g          673 t
ORIGIN      
        1 cagggcatgg accgcaattt ggtcgaagcg ggacaaatcg acggggcgac gcgctggcag
       61 aacctgcgct acatcgtgat cccgctgctt tatcccacca ttcgcctctc ggcatttttt
      121 gccatcgtcg gctcgctgca gctgttcgat ctgatcatgc cgctgacgcg cggcggtccg
      181 gcggattcct cgcataccat ggtgagtttt ctctacacct tcggcgtgat gcgcatgcgc
      241 gtcggcttcg gcagcgccgt cggcgtcatc ctgttctgca tctgcgtcac ctttgccttc
      301 acctacaaac gctggttcat gcgcgatgag tgatcaggcg acattctccg atcggggttt
      361 cgacctggcc gcgctgtaca aggcgctgtt cctgacggtc gttgtggttt tcgtcgcgac
      421 gccgctggtg gcgaccgtgc ttggcggctt caaatcgctc ggcgaattgc gcaccaatcc
      481 gttcggcctg ccgcaggtgt gggaatggga gaactacggc ggaattctac ggtcgaaacg
      541 gtactggcag ctgctcggca attcgctgat catttcgacc atgaccgtgg tactgacgct
      601 gatcgccgca tcgatggccg ctttcgtgtt cgcgcagatc aagtttttcg gcagcgccat
      661 gctgatgagc tatatcacca tgggcctgtt gtttccggcg gcgaccgcca tcctgccttt
      721 gttcatcaag gtgcgcgatc tcgggttgct cgactcctat ttcggcgtcg tactgccgca
      781 ggtggcgttt gcgctggcga tgagcatcct gttgctgcgg cgtttcttca aggacattcc
      841 gtatgaactt ctcgaagcag cccgaatcga cggctgcagc tatatcgagt ttttccgcta
      901 tgtgacgctg ccgctgtcgc gtccgattct cgcgaccgtc ggcaccatcg ttttcgtcaa
      961 cagctggaac tcgtatttga tcccgttgat catgctgaac agcgacgacc tctatccctg
     1021 gccactcggc atcatggtct atcagggcga atactcgtcg gaatggcacc tgatcctggc
     1081 gttcgtcacg ctgacgatcc tgccgaccat catcctgttt ctgttcgcgc aaaaacacat
     1141 cgtcgccgga ctgacggcgg gagcggtcaa gggctgagct ttcacaccaa ccaaactcaa
     1201 acggatcgaa gcggagtctc catgagaaaa gtcggcatcg gcgtcatcgg ctgcggcgtc
     1261 atcagcaagg cctacctgac ggccatgaag cagttttcga atatcgaact gcgcgcggtc
     1321 gccgatatgc ggtcggcggc ggccgagcag cgcgcaaagg aattcgacgt tcccggcatg
     1381 cgggtcgacc agatgctcaa acgcgatgac gtcgaaatcg tcgtcaacct gacggtgccg
     1441 ctggcccata ctgatgtcag cctcgcggtt ctgaacgccg gcaagcatgt ccattcggaa
     1501 aagccgctcg gcgtcaatgt cgccgaggcg cgcaaggtga tggatctcgc cgcccagaaa
     1561 aatctgcgcg ttggctgcgc gccggacacc ttcctcggcg gcgggcacca gaccgcgcgc
     1621 aagctgatcg acgacggggc gatcggcacg ccggtcggcg gcaccgcctt cttcatgtgc
     1681 cccggccacg aacgctggca tccggcgccc ggcttctatt atcttcgcgg cggcggcccg
     1741 atgctcgaca tgggccccta ttacatcacc gacctggttc agctgctcgg tccgatcgcc
     1801 tcggtgatgg gcgcggcgac aaaaccccgt ctggagcggt tgatcaccag cgagcccctc
     1861 aacggcacga tgattccggt ggaggtcgcc actcacgtta ccggcacgct cgaattcgtc
     1921 tcgggtgcgg tgattgccat caccatgagt ttcgatgtgc cgaagcacag ccacctgccg
     1981 atcgaaatct acggcaccgc cggcagcctg ctggtgcccg atcccaaccg tttcggcggc
     2041 gaggtcagcc tcgccaagac cggtggcgaa tggcagccac aaccccatac ccacggcaac
     2101 accgaaggcg aattccgctc catcggcgtc gccgacatgg cggcggccat tgtcggcaac
     2161 cggccgcacc gcgccagcgg cgcgctggcg ctgcatgtgc tggaggccat ggaggcgttt
     2221 cagatatcat cggaccaggg acgccgcata aaactcgaaa ccaccgtcga acgtccggcc
     2281 atgataccgc cgggacttgc taccggccaa atcaattaag caaaggatgc tgaaaatgcg
     2341 cgaggcgatg atcgtatggg gcggctggcc gggacacgat ccggatctgt gcgcctcgat
     2401 gatacgaggt tggctgaaag ccgaaggctt cggggtcagg atcgaaacca caaccgaggc
     2461 cttcctcgat ccggccctca agaacctctc gctgatcatt ccgatctaca ccatgtcgaa
     2521 gatcgagaag ccggaggcgc tggcgctgtg cgacgcggtc aagagcggcg tcggccttgc
     2581 cggacatcat ggcggcatgt gcgacgcgtt ccgcgacaac gtcgactatc agttcatgtg
     2641 cggcgggcaa tgggtggccc atcccggcaa catcatcgac tacaaggtcg acgtgaccag
     2701 gcccgatgat cccgtgatgg ccggcatcga cagtttcgaa caccgttccg agcaatatta
     2761 catgcatgtc gaccccgcca tcgaggtact ggcaacgacg accttcaacg ccggtcacgc
     2821 tccctgggtc gatggcgtcg tgatgccggt ggtgtggaaa aagcgtttcg gccaggggcg
     2881 cgtattctat tcgtcgctcg ggcatcgggc ctatgaactc gatgtccccg aaatcagaac
     2941 cctgatgacc cgcggcatgc tttgggcggc gcgatgagcg cggatggcga gcgaataccg
     3001 ggccggaggg cgacgcttga agacgtcgcc cgcgaagccg gtgtgtcgct ggccaccgcc
     3061 gaccgcgtcg tcaaccagcg cgagggcgtg cgcgggcaga ccatcgcccg cgtcgaactg
     3121 gcgatcgcca agctcggtta ccgggccaat gccgccgcca tgcggctcgc ccgcaacgag
     3181 agcttccgtt tcgccttcat cctgccctcc aacgccaaca gcttcatgtc caacctgtcg
     3241 gtgcaggtcg agcgcacctc cgattggctg gcaagccaga acggctatat cgacgtcatg
     3301 catgtcgatg tgttcgatcc gaatgcgctc gcttcggcgc ttgaacggct ttcgccaagc
     3361 tatca
//
LOCUS       DASWGM010000112         4987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1947226,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000112 DASWGM010000000
VERSION     DASWGM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947226"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VGA15_03355"
     CDS             <1..305
                     /locus_tag="VGA15_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEX9586765.1"
                     /translation="GDDVTHVITEQGVANLLLCRSPKEREGALRAIAGDTDFGRAQAR
                     DMTDNLRERRIVMQPSDLGIDAKDATRDLLSARTIDDLVVWSGGLYVPPLKFRTSR"
     gene            340..1704
                     /locus_tag="VGA15_03360"
     CDS             340..1704
                     /locus_tag="VGA15_03360"
                     /inference="COORDINATES: protein motif:HMM:NF017135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEX9586766.1"
                     /translation="MTSNVVETRLPRSNTSRLSNEAERPTAAADQFSRAKQLAESLLS
                     ERGEASGALLARELHGVLRGLNAGDRHGFQRYLATKFQPDTAALRVAAERYLADTTAE
                     AATALAQAADPPRQELLRRMNMAPGGTGTLIAMRSEITAHLRHEPELKLLDADLKHLF
                     ASWFNRGFLELRRIDWQSPAAVLEKLIAYEAVHAIKGWDDLRRRLAPDRRCFAFFHPA
                     LPGEPLIFVEVALVEGLATSISPLLSQDVEEDTARAQAAGADTAIFYSISNCQDGLRG
                     VSFGNFLIKQVVEELQTDFPQLKRFSTLSPVPGFRRWLTRQLAEQSNPDAALLPKLAR
                     DGWWHDPEQSEPLRAALMRLCATYLTRRASPGNRIDPVARFHLGNGARLEQINWLGNT
                     APRGIQESFGIMVNYLYDIETIESNHEAFVNDGTVMRSADVDALLAAEHGVELGVLRK
                     HTTP"
     gene            1935..2705
                     /locus_tag="VGA15_03365"
     CDS             1935..2705
                     /locus_tag="VGA15_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9586767.1"
                     /translation="MNRRAFVGMVAAGAASTLIQGTAAVAQPAPKARNVVLVHGLFAD
                     GSSWSEVIARLQAAGLNATAVQNPLTTLPEAVASAERVLARLDGPTVLVGHSFSGMIV
                     TEAGMHPNVSALVYVAARAPDAGEDYTALAKTFPTPPASAGIVFDGDEGRLSEAAFLR
                     DFAGDLPEAKAKVLYAVQEPFHKALLTGKTTHAAWRSKPSYYAVSTEDRTINPDLERF
                     MAKRMGAKTIEVKASHLSLISQPDEITRLILEAAGQPA"
     gene            2810..3235
                     /locus_tag="VGA15_03370"
     CDS             2810..3235
                     /locus_tag="VGA15_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX9586768.1"
                     /translation="MASLYSTKVTASGGRHGSIRSEDGLLDLKLALPRALGGRGDATN
                     PEQLFAGGYAACFENALLHVSRDAGHRFADDDIDVVARIDLSRNETGGFVLAAALAVT
                     MAGIDQQTAERLVQGAHAVCPYSNAIRSNIDVAISVSVR"
     gene            3282..4256
                     /locus_tag="VGA15_03375"
     CDS             3282..4256
                     /locus_tag="VGA15_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9586769.1"
                     /translation="MLMLDRRMLLRAGSAAAAAAALAPTAPGLAHAPQAGKRAQPSFY
                     RFKLGTIEITVVSDGTLAFPAETLWGDRAEDARGLLTSTFQPSSPVGLQINTILVNTG
                     DKLVLIDAGCGVDKFQKTAGGLLGNLAAAGYAPGDIDMILFTHCHFDHLWGISDGKNA
                     SLLFPSAEFVASETEVAFWSAPDLPGKVSPKQQPLVTQANLKLASPRLRLIKAGAEVA
                     PGVATFDTAGHTPGHISVHISSGSEEMLLTGDVVVNSAVSFLHPEWPFGFDLDVPLAT
                     KARMAFLDRAAADKTLVGSYHLPFPGFGHVLREGSGYRWLPADWQWTT"
     gene            4370..4756
                     /locus_tag="VGA15_03380"
     CDS             4370..4756
                     /locus_tag="VGA15_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX9586770.1"
                     /translation="MPFVTFTVRRGLSAADKSRLSEAMLEAQVAAGYHRADRFHRFLE
                     VDQDDLLVDPRFPDYATDRTDRFMVVEVVISSGRPTGTAATIADEAVRLFGERLHLAP
                     QDILFVLHEVEPNLPRFPAASIRPCG"
BASE COUNT          973 a         1606 c         1521 g          887 t
ORIGIN      
        1 acggcgacga cgtcacccac gtcatcaccg aacagggtgt cgcaaatctc ctgctgtgcc
       61 ggtctcccaa ggaacgtgaa ggcgcgctgc gcgccatcgc cggagacacg gactttggac
      121 gcgctcaagc tcgagacatg accgacaacc tgcgcgaacg ccggatcgtg atgcaaccgt
      181 ccgaccttgg gatcgatgca aaggacgcga cgcgcgatct gctctccgcg cggacaattg
      241 acgatctcgt tgtctggtcc ggcggcctgt atgtgccgcc actcaaattc cgcacgtccc
      301 ggtagcggcc tcgatcactc aagcaacaag gaagatgaga tgacatcgaa cgttgtcgaa
      361 acgcgccttc cgcgctccaa tacctcacgt ctcagcaacg aagctgagcg gccaactgcc
      421 gcggctgatc agttcagccg cgccaagcag cttgcggaat cactgctctc cgaacgcggc
      481 gaagcatccg gcgcgctgct ggcgcgcgag ctgcacgggg tactgcgcgg cctcaatgcg
      541 ggcgaccggc acggcttcca gcgctatctg gcgaccaagt ttcagcccga cacagcggca
      601 ctgcgtgtcg cggcggaacg ctacctcgcc gataccacgg ctgaagccgc gaccgccctc
      661 gcgcaggccg ccgatccgcc ccggcaggaa ctgctgcggc ggatgaacat ggcgcccggt
      721 ggcaccggca cactgatcgc gatgcgcagc gagatcaccg cgcatttgcg ccacgagccg
      781 gaactgaagc tgctcgatgc cgacctcaag catctgttcg catcctggtt caaccgcggc
      841 tttctcgagc tgcggcggat cgattggcag tcgccggcgg cggtgctgga gaagctgatc
      901 gcctatgagg cggtgcacgc gatcaagggc tgggacgacc tgcggcgccg tctcgcgccc
      961 gaccggcgct gcttcgcctt tttccatccc gcgcttcccg gcgaaccgct gatcttcgtc
     1021 gaggtcgcgc tggttgaggg actggccacg tcgatatcgc ctttgttgtc gcaggatgtg
     1081 gaggaggaca cggcgcgggc gcaggcggca ggcgcggaca ccgcgatctt ctattcgatc
     1141 tccaactgtc aggacggcct gcgcggcgtc tcgttcggca acttcctgat caagcaggtg
     1201 gtcgaggagc tgcagacgga ctttccgcag ctcaagcggt tttcgacgct gtcgccggtg
     1261 ccgggcttcc ggcgctggct cacgcgacag cttgcagagc aaagcaaccc cgatgccgcg
     1321 ctgctgccga agctcgcgcg cgacggctgg tggcacgatc cagagcaaag cgaaccgctg
     1381 cgcgcagcct tgatgcgcct ttgtgcgacc tacctgacgc gacgggcatc gcccggaaac
     1441 cgcatcgacc cggtagcgcg ttttcacctc ggcaacggcg cgcggctcga gcagatcaac
     1501 tggctcggca acaccgcgcc gcgcggcatc caggaatcgt tcggcatcat ggtgaactac
     1561 ctctacgaca tcgaaacgat cgagagcaac catgaggctt ttgtaaatga tggaacagtg
     1621 atgcgttctg cggacgtcga tgcgttgttg gcggccgaac acggcgttga gcttggagtc
     1681 ttgcggaaac acacaacccc gtgacgccgc gtaacaatag gcctcttcct tcagaatata
     1741 gatgcgggaa aaggctcggg cgaaaagctc tcacggagcg atcgatgaca atgttgcgaa
     1801 cactgagcat tgttaccggc attcctgcgg cagattttcc aagtagccaa cgtcaactct
     1861 cccagtagcc aaccaacttt acgcaaagga gaacttccat gagtaaccaa gacgatcttc
     1921 ggccaaggta tccaatgaat cgacgcgcat tcgtgggcat ggtggctgcg ggtgcggcaa
     1981 gcactcttat ccagggcacc gccgccgttg cgcagcccgc gccgaaggcc cgaaacgtcg
     2041 tccttgtgca cgggctgttc gccgacgggt caagctggtc cgaggtgatt gcacgattgc
     2101 aggcagcggg cctcaatgcc acagccgtgc aaaacccact gacaacgctg cctgaagcgg
     2161 tagcctcggc cgagcgcgtg ctggcgcgac tggatggtcc gacggtcctg gttgggcatt
     2221 ccttctccgg aatgatcgtg acagaggccg gtatgcatcc gaacgtgtcg gctcttgtct
     2281 atgtggcggc gcgagcgccg gatgcgggcg aggattacac ggcgttggcc aagacatttc
     2341 cgacgccacc ggcgtccgcc gggattgtct tcgacggcga tgaaggacgc ctcagcgagg
     2401 ctgcgttctt gcgcgatttc gcgggcgacc tgccggaagc gaaggcgaag gtgctctatg
     2461 cggtccaaga gccgttccac aaggcgctgc tcaccggcaa gaccacgcac gcggcgtggc
     2521 gctcgaagcc gagctattac gccgtttcaa ccgaagaccg cacgatcaat cccgatctcg
     2581 aacgcttcat ggccaagcgc atgggcgcca agaccatcga agtgaaagcg agtcacctgt
     2641 cgctgatatc ccaacccgac gagattaccc ggttgatcct ggaagccgcc ggacagccag
     2701 cttagcaggt gtgcgtcctc gtgcacgggc tgttggctga tgagttgcgg cgaagtccag
     2761 acgaccggtc tcaaaattag cgaatcgcag cgaagaaggc atatgtgcaa tggccagtct
     2821 atattccaca aaagtcacgg cgagcggagg ccgtcacggt tcgatccgca gcgaagacgg
     2881 cctgctcgac ctcaagctcg cactgcctcg ggcgcttggc ggtaggggcg atgcgaccaa
     2941 tcccgaacaa ctcttcgccg gcgggtatgc agcctgcttt gagaatgcac tccttcatgt
     3001 gagccgggat gccggccacc gctttgccga tgacgatatc gacgtcgtcg ccaggatcga
     3061 ccttagtcga aatgaaacag gaggcttcgt gttggccgcc gcgcttgccg tgaccatggc
     3121 tggcatcgac caacagaccg ctgaacggct cgtccagggt gctcatgcgg tctgcccgta
     3181 ctcgaatgcg atccgaagca acatcgatgt ggcgatctcc gtctcggtcc gatgagcccg
     3241 atcgatcgat ttcataaccc aactagcaat ggagaacaga catgctcatg ctcgaccgcc
     3301 ggatgctcct gcgtgccggc tctgccgccg ccgcggccgc tgcactcgcc cccaccgccc
     3361 ccggcctggc ccatgcgccc caagcgggca agcgggcgca acctagcttc taccgcttca
     3421 agctcggcac catcgagatc accgttgtca gcgacggcac gctcgcgttc ccggccgaaa
     3481 cgctgtgggg cgatcgggcg gaggacgcaa gaggcctgct cacatccacg ttccagccat
     3541 ctagcccggt ggggctacaa atcaacacca ttctcgtcaa caccggcgac aagctggtgt
     3601 tgatcgacgc aggctgcggc gtcgacaagt tccagaaaac ggccggcggg ttgcttggca
     3661 acctcgccgc cgcgggctac gcgccgggcg atatcgacat gatcctgttc actcactgcc
     3721 acttcgacca cctgtggggt atcagtgacg gcaagaacgc gtcgctgctg ttcccctcgg
     3781 ccgaattcgt cgctagcgag accgaggtcg ccttctggag cgctcccgat ctgcccggca
     3841 aagtgtcccc caagcagcag ccgctggtta cccaggcaaa tctgaaattg gccagccctc
     3901 ggctgcgcct gatcaaggcc ggagcggagg tggcgccggg cgtggccacc ttcgacaccg
     3961 ccggtcacac gccagggcat atctcggtgc acatcagttc cggcagcgaa gaaatgctcc
     4021 tcaccggcga cgtcgtggtc aactcggcgg tcagcttcct gcatccggaa tggccgttcg
     4081 ggttcgacct ggacgtgcca ctggcaacaa aggcgcgcat ggctttcctc gaccgcgcag
     4141 cggccgacaa gacgctggtc ggcagctacc atctaccctt tcctggcttc gggcacgtac
     4201 tgcgcgaggg gagcggctac cgctggctgc cggcggattg gcagtggacg acctgacgac
     4261 cacgccgggc gggggcggtg tgcctctcaa attgcaggat ggcggctaag gcgtgcgcgc
     4321 acttagcgca tcaacgcaat tggtacatcc taagccgtgg agactggaga tgccgtttgt
     4381 aaccttcacc gtccggcgcg gcctcagcgc cgccgacaag tcccgcttat cggaggcaat
     4441 gttagaagct caggtggccg ccggctatca ccgagccgac cgctttcacc gctttctcga
     4501 ggtcgaccag gatgaccttc tggtagatcc ccgcttcccc gactacgcaa cggacagaac
     4561 cgatcggttc atggtcgtcg aggtagtcat ctccagcggg aggccgacag gaaccgccgc
     4621 caccattgcg gacgaagcgg tcagactgtt cggcgagcgc ctgcacctcg cgcctcagga
     4681 catcctgttc gtcttgcacg aggtggaacc gaatctcccg cgctttcctg ctgcgtcgat
     4741 caggccatgc ggctgagtgt gcggcagatg tcgatgccga ccttctcaac gacgcccgca
     4801 gcaacttcca gccgttaccc aaggatatgg cgacgcccga gttcccgatc acgccttcgc
     4861 gggttgagct cggccgaaat agcttgagat ctgtacatcc tacgtgagaa gggcaacgaa
     4921 aatgaatatc ttgaacatag tgacctcgcc acgaaaggaa aaatctgcgt ccaccgctgt
     4981 cgtcaat
//
LOCUS       DASWGM010000113        15953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2503591,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000113 DASWGM010000000
VERSION     DASWGM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15953
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503591"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..581
                     /locus_tag="VGA15_03385"
                     /pseudo
     CDS             109..581
                     /locus_tag="VGA15_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642661.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
     gene            1020..2033
                     /locus_tag="VGA15_03390"
     CDS             1020..2033
                     /locus_tag="VGA15_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586771.1"
                     /translation="MNQEQCVAISSLALGELLKSRCARALGTAGLVFTLGVAASGAAG
                     AQTAPVGVPDRSFPESVTSTKDGTLYVGSFNLGGVVKAVPGGKAEQFIKPGAAGSRSV
                     LGVLADEKSGMLYVCSNDITGFGVPDPGDTKGAWLKIFDLASGAPKGSFALSDPKSLC
                     NDIAVGADGTAYVSDSFTPNVYSLKPGGTALEVWATDPMFAPAKDGVGLDGIAFGADG
                     NLYVTTYIPAALFKIAVKDGKAGAITALKTSRQLDHADAMRTFGDSLLLIEGNGKLDK
                     VTIKGDEAQIETIKDGFAEPVSVTQVGNIAWVAEGKLSYIIGDNKEKDPGPFAIKPVM
                     LSK"
     gene            complement(2229..3191)
                     /locus_tag="VGA15_03395"
     CDS             complement(2229..3191)
                     /locus_tag="VGA15_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9586772.1"
                     /translation="MTAQLDPVIEKIIPLLPLRDPETMTPQSARDALRALAAARAAIP
                     PPQVASADDTKVKGAAGPLAARVYRVSREKSPTVVFFHGGGWVAGDLETHDRQARLLA
                     IETGAVVVSVDYRRPPETRFPGAFEDGFAAVRDIFGRIAEFGGDNARVGVAGDSAGGN
                     LAAAVAIACRDASVTLAAQLLVYPVTDVAGNFADAKENARFPSRTENAEGYFLSRAVM
                     QWFANHYLSDATHGADWRVSPLRAKSLAGLAPAIVCTAWFDPLRDEGKAYADALAAAG
                     VATKCHPGAGLIHGYFGLADASETARREAQRARADFKALLERGA"
     gene            complement(3292..4488)
                     /locus_tag="VGA15_03400"
     CDS             complement(3292..4488)
                     /locus_tag="VGA15_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9586773.1"
                     /translation="MAHDEPQAAGPTKLVIRNIGLLLSGAMEKPILDADTILAENGKI
                     TVIGRAKDIDAEGATTIVDANGTTVAPGLIDSHVHPVAGDWTPRQNQINWIDSFLHGG
                     VTTMISAGEVHMPGRPRDVVGLKAMAIFAQRSFWTLRPGGVKVHAGAPVIENEMVEDD
                     FRELAAAGVKLLGEVGLGGVKDGPTARKMVGWARKYGIQSTIHTGGPSIPGSGLIDKD
                     VVLEADTDVIGHINGGHTALPDDQIRCICEGCKRGLELVHNGNERSALYTLRIAREMG
                     DLSRVILGTDSPAGSGVQPLGILRMVSLLSSLGEIPAEIAFCFATGNTARMRALDCGI
                     IEVGRSADFVIMDRAQHSPGKNILDSVQLGDLPGIGMTIIDGIVRTQRSRNTPPATRV
                     PAIVGH"
     gene            4663..5436
                     /locus_tag="VGA15_03405"
     CDS             4663..5436
                     /locus_tag="VGA15_03405"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX9586774.1"
                     /translation="MSSFNQESVLSVHHWTDNLFSFTTTRDPSFRFRNGEFTMIGLKV
                     GEKPLLRAYSVASANYEDRLEFFSIKVPDGPLTSRLQHLKQGDEVIVSRKPTGTLVID
                     NLEDGRNLYLIGTGTGLAPFLSVIKDPETCARFEKVVLLHGCRRVAELAYGEMITKEL
                     PNDDLIGELVRNQLIYYPTVTRDPFRNRGRITDLITSGKLFSDAGLAPLESEHDRVMI
                     CGSPALVHDTRALLSGKGFVEGNHGEPGQFLVEKAFAER"
     gene            5683..5832
                     /locus_tag="VGA15_03410"
     CDS             5683..5832
                     /locus_tag="VGA15_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586775.1"
                     /translation="MSLTTMLMNNKTQPADRGAAIYKALWHAIIEQALQPGAKLPEGA
                     IGGEK"
     gene            5842..7161
                     /locus_tag="VGA15_03415"
     CDS             5842..7161
                     /locus_tag="VGA15_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9586776.1"
                     /translation="MSFDTILRNGRIAGREHEPVDIAVCDGRIAAITPRSSQTTRDAA
                     CDIALHGKLVIPGFVETHIHLDKSCISDRCSCRAGTLQEAIESVAAAKLAFTEQDIYA
                     RGRRTLEKAIVQGTTRMRAHVEVDPRIGLKGFRAVSQLKRDFAWALDIEICVFPQEGL
                     LNDPGCDELLVQACDGGADLIGGCPYTDSDPHGQIARIFELAKRYDLDIDFHLDFDLD
                     PSWMHLDEVCGQTAKYGWGGRVAIGHVTKLSALDHAALEKIGVKLANAGAAVTVLPAT
                     DLFLMGRGAEHSVPRGVAPAHRLLAQGVTCSLATNNVLNPFTPFGDCSLLRMANLYAN
                     IAQLGRSEELQACMEMVTRLPAKLMNLGDYGIAVGNPADLVVLDCADRVSAVAELAQP
                     LLAMKRGRISFSRPTATLHWPGATPSLKDDFRAAPFAEARSVARSLL"
     gene            7237..8067
                     /locus_tag="VGA15_03420"
     CDS             7237..8067
                     /locus_tag="VGA15_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX9586777.1"
                     /translation="MADLSKSVRADLRVAIAGLGPIGKRVAEALDRGIDGLALVAVSA
                     QHPEKHRSWLSGLTRAPAVLPIDALCDVADIVIECAPGKLVRSIVEPFVTSGKIAVVL
                     SAGALLDHEDLIELAKRNGGQIVVPTGALIGLDAVTAAAVGTIHSVRMVTRKPIAGLA
                     GAPYIIENNIDIERITEPLRIFDGTAREAAKGFPANLNVAVALSLAGIGPDRTKLEIW
                     ADPTVTRNIHRIEVESCSARFSMSIENIPSENPKTGRITALSVIAYLRKQRASLRVGT
                     "
     gene            8379..8588
                     /locus_tag="VGA15_03425"
     CDS             8379..8588
                     /locus_tag="VGA15_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586778.1"
                     /translation="MSAFSSDWRQATAVQLYTVRNVFPFLQSELGKRGTLRPGLTWHF
                     DRPPVVDLEYEMDCRRVHIERVIEV"
     gene            8715..9335
                     /gene="pncA"
                     /locus_tag="VGA15_03430"
     CDS             8715..9335
                     /gene="pncA"
                     /locus_tag="VGA15_03430"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEX9586779.1"
                     /translation="MQIRDDDLLLVIDVQNDFCAGGALAVADGDAVVPIVNRLTERFN
                     HVALTQDWHPSGHSSFATSHPGSAPFRTIRMPYGEQTLWPDHCIQGAPGAAFHPQLVT
                     ERAELVIRKGFRPEIDSYSAFYENDRRTPTGLAGYLRERGLRRIFLAGLATDYCVYYS
                     AVDARRLGFDTVLIEAACRAIDLAGSLDAAWAGMATAGVQRVNDLS"
     gene            9561..11519
                     /locus_tag="VGA15_03435"
     CDS             9561..11519
                     /locus_tag="VGA15_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9586780.1"
                     /translation="MRRILLALAMALTVIALPGVTFAISDEETVRLLKHDADQGNANA
                     QGRLGYFYETGRGGLAKDDREAARLYRLAADQGNASARNNLGGLYRDGRGGLTKDDRE
                     ALRLFKLAADQGNAYGQLNLGLFYREGRGGLSKDDREAARLFKLAADQTQADARVNLG
                     YFYETGHGGLAKDDHEAVRLYRLAADQGNATAQSNLGVFYRDGRGGLTKDDREALRLF
                     KLAADQGNPYGQNQLGGFFRDGGGGLAKDDREAARLFKLAADQGNASARVNLGYFYEK
                     GRGDLAQDDREAARLYKLAADQGNAAGQTYLGVLYRDGRGGLTKDEREAARLFKLASD
                     QNYAYGQQNLGIFYRDGRGGLTMDDREAARLLRLAADQGNANARTALNQITQQPSQEV
                     SSAPSAHLKQEVVEPSSAPAANLPSVPERRVALVIGNSAYQTVSGLPNTTNDARAIAE
                     VLKADGFTSVRIILDMTRASMIASLNDFQREADTSDWAVVYYAGHGLEVSGANYLVPI
                     DAKLGDDRDIQDEAVSVDRILSAVENAKKLRLVMLDACRDNPFLKRMHRSIATRSISR
                     GLAAIEPMGATLIVFAAKDGETAEDGTGGHSPFAASLIKRLQQPTIEINKLFRLVTSD
                     VLKATGNQQRPFVYGSLPGEEDYYFRLR"
     gene            complement(11548..12147)
                     /locus_tag="VGA15_03440"
     CDS             complement(11548..12147)
                     /locus_tag="VGA15_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX9586781.1"
                     /translation="MTRPSPFSVRGRRALIVMRDEREISIVRRQFSRLGMTIAEHDPA
                     RAPPDENADVVVMDADSIPIKSDLAAAWKAGAPIIALIGTETPSRLKWLLDLQPASFL
                     IKPLHSAGLYTALVVAFDSAQRRIDETAHLERLEDRIRSRRVVFAAVLQIMRAHDLSE
                     SDAFRLIRQTAMRHRTTIEQLSAEIIAAGGMPNRTSRTA"
     gene            complement(12144..13325)
                     /locus_tag="VGA15_03445"
     CDS             complement(12144..13325)
                     /locus_tag="VGA15_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9586782.1"
                     /translation="MKKPSVPLGLVFSQSGPYAMMAGEMRKSALMAIDEVNRSDEFDF
                     TFTPHLRDPGGVVAAYHTACDDLIREEQVDHIVGCYTSASRKQVIPIVERTERLLWHP
                     ARYEGFESSDNVIYVGAAPNHNVVPLVRHMLDQVSSDVFCIGSNYVWTWETNRVIREI
                     VTSAGGRILAERLLELGETAVDHIVREIIDRKPPAVFNTLVGESSYVFVRALHEAMVR
                     ARLTIPMLSCSLCEPELKLIGPAASVGCITSSAYFESIERPENRAFVARWKARHGADS
                     NPSVDGQSTYVCVMLLARAIRRAGSADVGAVRCAAANHRYDSPQGPVWVDPDNNHCFL
                     TPRLARSVPGCQFKIFWEADAPERPDPYLSNLDLTAVGTRLERSNDAMTKRSSHLRIV
                     K"
     gene            13681..15198
                     /locus_tag="VGA15_03450"
     CDS             13681..15198
                     /locus_tag="VGA15_03450"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9586783.1"
                     /translation="MVVRLPTLEQIDNLGSDFGLVLNADEVAAFQQAFKGPLASYGRL
                     DELVPPALAPVAPRSPGYRPTASENPYGAWYWKTNIQTGAEGLLSGKQVAIKDNICVS
                     GVPMMNGSVLLEGYVPELDATVVTRILAAGGTIAGKAACEDLCFSGASHTCASGVIRN
                     PHNPAHSAGGSSGGSAALVAAGEVPMALGGDQGGSIRTPSSWCGIYGLKPTWGLVPTT
                     GSMPISYSVDHCGPMGASVEDVARLLTVIAGHDGWDTRTISARTGDYMAALGKPAKGL
                     RVGILREGFGHPESDPAVDAKVRATIEALAKSGVEAAEISVPWHLDGPHVWSGIILEG
                     AAEMMLKGYGVGNNIHGYYPLSMQEAFARGMGTRINDVSPTVKLVLMLGEYMHRNYHG
                     RYHSKAQNLRVLLRRAYDEALEKFDVLAMPTIPFTATPIPPADAPLGTTIDVALNMQA
                     NTCSFDVSGHPAFTVPCGRVNGLPVGLMLVGRHFEETTLIRLASAIEAGGDWKLN"
     gene            15217..>15953
                     /locus_tag="VGA15_03455"
     CDS             15217..>15953
                     /locus_tag="VGA15_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9586784.1"
                     /translation="MPTRDLHYCELVEIGQRIQKRELSPVEATQAQLDRIARLDGQLK
                     SYAYVMASSALEQAHAAEREIGSGKVRGPLHGVPIAVKDLCWTKDAPTAAGMRIYRDN
                     RPTQDATVVTRLKEAGAVILGKLQLTEGAYADHHPDIDPPRNPWDATLWSGASSSGSG
                     VATAAGLCYGSLGSDTGGSIRFPSAANGVTGLKPTWGRVSRYGVFELAATLDHIGPMA
                     RSAVDCGAILGAIAGADPNDPTAVLEPV"
BASE COUNT         3117 a         4738 c         4970 g         3128 t
ORIGIN      
        1 tgaagctcaa ggacggcgtc gtgatggtcg cttcgacctt ttcgggtcac gtcggcgtca
       61 cgcaaagctg atccgcgcct cacattgcga ccgtccgcgc gccgagagcc cgccaacgac
      121 aacttctgcg gaggtttcag cagcgagaag gtgccgctgg atcccggcaa gctctcggcc
      181 tcaaggtgcg cccgggcgcc gatcattatg gtgaactgca agcgtgggat ccggccaccg
      241 gaaagaaggt ctggcagtac aatttcccca aatcacagct gtttggttcg gtgactgtaa
      301 ccgccggcga tctcgtcctg gtcggaggta cgaatgaccg gatgttccgt gcctttcatg
      361 ccaagactgg cgaattgctt tgggagcaaa agaccaactc cggcatcatg ggcatgccaa
      421 tcgcgtttga agtcgatggg acggaatata tcgccgtcca gtcgggttgg ggcgtagacg
      481 cgcagcgcat ccaggacgca ttggcgacca gtaacaatat tggggtggag aacaacgtgc
      541 ctcaaggcgg cgtcgtttgg gtattcgccg tcaagaagta atggtcgaga aatatcctgg
      601 ccgcttcagg cagcggccat gtgacttcct gaaatccaga ctttgatgcc atgatctcgg
      661 cgtcgccgag atcatggctg gccaaccatt gcatcaccgc gcgcgacggc gaatagccag
      721 tcgcgctttt ggcgcatgag ggaatgcgcg ttctccttcg cgttcgcgtg cggtcgattg
      781 gggccgactt ctttcgcaga ctgacgcgat ttgtgccgcg gaatcggact tgactgcgat
      841 gttgtcgctt tcaagttttc gaaccgtatc ctagaattaa ttatcgggat aattgcgggc
      901 tgccatcgtt ggatcgcagc ccttcgctgc ccaatcgcga cgcagcgaac cgatagctgg
      961 tgatgatcgg cccctgaggc attgcctcgt ccacctcatg tcgatcagga atctcaatca
     1021 tgaatcaaga acaatgcgta gctataagct cgcttgcact gggagagttg ctcaagagcc
     1081 gctgcgcgcg tgcgctcggc acggccggac tggtattcac gctcggcgtc gcggctagcg
     1141 gcgctgcggg tgcccagacc gcgccggttg gcgtcccgga tagaagtttt ccggaaagcg
     1201 tgacatccac caaggacggc acactctatg tgggcagctt caaccttggc ggtgtggtga
     1261 aagccgtgcc cggcgggaaa gcagagcagt tcatcaagcc cggcgccgcc ggcagtcgtt
     1321 cggtgctcgg cgtgcttgcc gacgagaaaa gcggcatgct gtatgtctgc tccaatgaca
     1381 tcaccggctt tggcgttccc gatcccggcg acaccaaggg cgcctggctg aaaatcttcg
     1441 atctcgccag cggcgcaccg aagggcagtt ttgcactcag cgatccgaaa tccctgtgta
     1501 acgatattgc cgtcggcgct gacggcactg cctatgtctc cgactcgttc acgcccaatg
     1561 tgtacagcct gaagccgggt ggtaccgcgc tcgaggtctg ggcgacggat ccaatgttcg
     1621 caccagccaa ggacggcgtc ggcctcgacg gcatcgcgtt cggggcggac ggcaatctct
     1681 acgtcacaac gtatatcccg gccgcgttgt tcaagatcgc cgtcaaggac ggcaaggccg
     1741 gcgcgatcac cgcgctgaaa acgtctcgcc agctggacca cgcggacgcg atgcgcacct
     1801 tcggtgacag ccttttgttg atcgagggga acggcaaact cgacaaggtc acgataaaag
     1861 gcgacgaggc ccaaatcgag accatcaagg acggcttcgc tgagcctgtc tcggtgacgc
     1921 aggtcggcaa tatcgcgtgg gtcgcggaag gcaagttgtc ctacatcatc ggcgacaaca
     1981 aggagaagga tcccggcccg ttcgcgatca agccggtcat gctttcgaag tagggcgctg
     2041 attgagagta acgtgccgca aggcagcgtc gtctggatgt tcgccgtcaa gaagtaattt
     2101 ggcaacgaac aatatctaat atcctggccg cttcgggcag cggccaggcc acttccaaat
     2161 cccaggtgtc tgcggggagt ttggacttcg cgggaaatgc tgcgccttgc cagcgaaagg
     2221 cgcagcatct acgctccgcg ttccagcagc gccttgaaat cggcgcgcgc gcgttgcgct
     2281 tcgcgccggg cggtttccga cgcatcggcc agaccaaaat agccgtggat caggccagcg
     2341 ccaggatggc atttggtcgc gacgccagcg gccgcaagcg catcggcata ggccttgccc
     2401 tcatcgcgca aggggtcgaa ccatgcggtg cagacgatcg caggtgcgag cccggccagg
     2461 cttttggcgc gcaacggcga aacgcgccag tccgcgccgt gcgtggcgtc gctgagataa
     2521 tggttggcga accattgcat caccgcccgc gacaggaaat agccttcggc gttttctgtg
     2581 cgtgagggaa agcgtgcgtt ctccttcgcg tcggcaaaat ttccagcgac atcggtcacg
     2641 ggatagacga gcaactgcgc ggcgagtgtg acgctagcgt cgcgacaggc gatcgccaca
     2701 gcggcagcga gattgccgcc cgcgctgtcg ccggccactc cgacgcgcgc attatcgccg
     2761 ccgaactcgg cgatgcgccc gaaaatgtcg cgcacggcag caaaaccatc ctcgaaggcg
     2821 ccgggaaaac gcgtttccgg cggacgccga tagtcgacgg agacgacgac cgcgccggtc
     2881 tcgatggcga gcagacgcgc ctgccgatca tgggtttcga gatcgccggc gacccatccg
     2941 ccgccgtgga agaacaccac ggtcggcgat ttttcgcgtg agacgcggta gacgcgcgcg
     3001 gcaagcggcc cggccgcgcc cttcactttg gtatcgtccg cgcttgcgac ctgcggcggc
     3061 ggaatggcgg cacgcgccgc ggccagcgcg cgcagcgcat cgcgggcact ttgcggggtc
     3121 atcgtttcag gatcgcgcag cggcagcagc ggaatgattt tttcgatgac gggatcgagc
     3181 tgtgcggtca tgggccctcc aggggccggg aaccaggcga gcacaggttt cgctcgccgc
     3241 ttcggccatt tgtcgtctgg aataggtttt cggattgaac ccacggatgt gtcaatgccc
     3301 gacgatcgcc ggcacccggg ttgctggcgg cgtgttgcgg ctgcgctggg tgcggacgat
     3361 gccgtcgatg atggtcatgc cgatgccggg gagatcgccg agctgcacgc tgtcgaggat
     3421 gttcttgccg ggcgaatgct gcgccctgtc catgatgacg aagtcagcgg agcggccgac
     3481 ctcgataatg ccgcagtcca gcgcacgcat ccgcgcggtg ttgccggtcg cgaagcagaa
     3541 cgcgatttcc gccggtatct cgccgagcga tgacagcagc gaaaccatcc gcaagatgcc
     3601 cagcggctgc acgccggagc cggccggcga atcggtgccg aggatgacgc ggctgagatc
     3661 gcccatctcg cgcgcgatcc gcagagtgta aagcgcggag cgctcgttgc cattgtgaac
     3721 cagttccaga ccgcgcttgc agccctcgca gatgcagcgg atctgatcat cgggcagggc
     3781 ggtgtggccg ccattgatgt ggccgatcac gtcggtgtcg gcttccagca ctacgtcctt
     3841 gtcgatcagg ccggagcctg gaatcgatgg gcctccggtg tggatggtgc tttgaattcc
     3901 atacttgcgc gcccatccga ccatcttgcg cgcggtcgga ccatccttga cgccgccaag
     3961 gccgacctcg ccgagtaact tgacgccggc cgccgctagc tccctgaaat cgtcttcgac
     4021 catttcattt tcgatcacgg gggcgccggc atggaccttt acgccgccgg ggcggagcgt
     4081 ccagaacgag cgttgtgcaa aaatcgccat cgctttcagg ccgacgacgt cgcgtggacg
     4141 gccgggcatg tggacttctc cggcggagat catggtggtg acgccgccgt gcagaaagct
     4201 gtcgatccag ttgatttgat tctgccgcgg cgtccagtcg ccggccacgg gatgcacatg
     4261 gctgtcgatc aggccgggtg cgacggtcgt gccgttggca tcgacgatgg tggtggcgcc
     4321 ttcggcgtcg atgtccttgg cgcgtccgat gaccgtgatc ttgccgttct cggccagaat
     4381 cgtatcggca tcgaggatcg gcttctccat cgcgcccgag agcagcaggc cgatattgcg
     4441 gatcaccagc tttgtcgggc cggcggcctg gggttcatca tgggccatgg atttattcct
     4501 ttttactcaa tgccttcgcg atcgtggacg gcaacttgag cccgacttga cgccgggatc
     4561 aagttggaat attcatttgc atacaaatga atattggatt gcgattggca accccgcaag
     4621 ccccggattt cgaccgcccc ggccttgctc ggatcaagca gaatgagcag cttcaatcag
     4681 gaaagtgttc tcagcgtcca tcactggacc gataatttgt ttagctttac gaccacccgc
     4741 gacccgtcgt tccgttttcg taatggcgaa ttcacgatga tcggcctcaa ggtcggcgag
     4801 aagccgctgc tgcgcgccta cagcgtcgcc agcgccaatt acgaggaccg gctcgaattc
     4861 ttttcaatca aggtaccgga cgggccgctg acctcgcgtc tgcagcatct gaagcaaggc
     4921 gacgaggtca tcgtcagccg caagccaacc ggcacattgg tgatcgacaa tctggaggac
     4981 ggccgcaatc tgtatctgat cggcaccggc accgggctcg cgccgttcct cagcgtgatc
     5041 aaggacccgg agacctgcgc gcgcttcgaa aaggtggtgc tgttgcacgg ctgccgccgc
     5101 gttgccgaat tggcctatgg cgagatgatc accaaagaat tgccgaacga cgatctgatc
     5161 ggtgaactgg tccgcaacca gttgatctac tatccgaccg tgacgcgcga cccgttccgc
     5221 aaccgcggcc ggatcacaga cctgattact tcaggcaagc tgttcagcga tgcaggtctt
     5281 gctccgctgg agtccgaaca tgaccgcgtc atgatctgcg gcagccccgc gctggttcat
     5341 gacacgcgag cgctgttatc aggcaaaggt tttgtcgaag gcaatcacgg cgagccggga
     5401 cagtttttgg tcgagaaggc attcgcggaa cgctaaagcg cggcgcaccg tattcctctg
     5461 attgccgtta tcttgccgga taaaccgccc cggttttgat ccaggcctcg acaagtgcgc
     5521 cggcttcttt tccagccctc cgatttgtca ggttattgcc cggtcagggc ctagccgagc
     5581 atcggtcgtc atgctttgat caagctcagc cgaatgtaat gaccgaggcg cgtgttgaat
     5641 ggcgggcgcc tcgaacctca atcggcgcgt tgatgacggc ggatgtcgct gacgacgatg
     5701 ttgatgaata acaagaccca accggccgat cgcggtgcgg cgatctacaa ggcgctctgg
     5761 cacgccatca tcgagcaggc gcttcagccc ggcgcgaagc ttcccgaagg tgcgatcggc
     5821 ggcgagaaat aggcctcttg catgtcattc gatacaattc tgcgcaatgg acgcatcgcc
     5881 ggccgcgaac acgagccggt cgacatagcc gtgtgcgatg gccggattgc cgccatcact
     5941 ccccggtcga gccagacaac ccgggatgca gcctgcgata tcgcgcttca cggaaagctt
     6001 gtgatcccgg gtttcgtcga gactcatatc catctcgaca aatcatgcat ctccgaccgc
     6061 tgcagctgcc gcgccggcac cttgcaggaa gcgatcgaga gcgtcgctgc ggcgaagctt
     6121 gccttcaccg agcaagacat ctacgcgcga gggcggcgca cgctggaaaa agcgatcgtg
     6181 cagggcacga cccggatgcg cgcccatgtc gaagtcgatc cgcggatcgg actgaaaggt
     6241 tttcgtgccg tcagccagct gaagcgggat ttcgcctggg cgctggacat cgaaatctgc
     6301 gttttcccgc aggaagggct gctcaacgat cccggctgcg acgaattact ggtccaggct
     6361 tgcgatggtg gcgctgatct gatcggcggg tgcccctaca ccgacagcga tccgcacggg
     6421 caaatcgcgc gcatcttcga acttgcgaaa cgttacgatc tcgacatcga ctttcatctc
     6481 gatttcgatc ttgatccgtc ctggatgcat ctcgatgagg tttgcgggca gaccgcgaag
     6541 tacggctggg gtggacgcgt cgccatcggc cacgtcacca aactgtctgc gcttgaccat
     6601 gccgcgctgg aaaagatcgg cgtgaaattg gccaatgcgg gtgccgcggt gaccgtgtta
     6661 cccgcgaccg atctgtttct gatgggccgc ggcgccgagc attcggtgcc acgcggggtg
     6721 gcgcccgcgc accgcctgct cgctcagggc gtcacttgtt cgcttgccac caacaatgtt
     6781 cttaatccgt ttacgccgtt tggcgattgc tcgctgctgc ggatggcaaa cctctatgcc
     6841 aatatcgcgc agctcggacg gtccgaagaa ctgcaggcgt gcatggagat ggtcacgcgg
     6901 ttgccggcaa agctgatgaa tcttggggat tacggcattg ccgttggcaa tccggccgat
     6961 ctcgtcgtgc tcgattgcgc cgaccgggtg tcggcggtcg ccgaattggc gcagccgctg
     7021 ctggcaatga agcgtggacg gataagcttt tcccggccga ccgccaccct ccattggcca
     7081 ggcgcaacgc cgtcgttaaa agatgacttt cgagccgctc cgttcgccga agcgaggtct
     7141 gtcgcccggt cgctgctgta gttgtttgcc aggattaagc gaggcccggc gcgcggaacg
     7201 ccgtgacggt gctgccatcg gaggatcgag acgctaatgg ccgatttatc aaagagcgta
     7261 cgagccgact tgcgagttgc cattgccggg ttagggccga tcggaaagcg ggtagcggaa
     7321 gcgctggacc ggggaataga cggattggct cttgtcgctg tgtcggcaca acatcccgag
     7381 aagcaccgaa gctggctttc cggtctaacg agagcgcccg cggtcttgcc gatcgacgcg
     7441 ctctgcgacg tcgcggatat cgtcatcgag tgcgccccgg gcaagctcgt cagatcgatc
     7501 gtcgaaccgt tcgtaaccag cggaaagatc gcggtggttc ttagcgcggg cgcgctgctc
     7561 gaccacgagg atctcatcga gcttgccaag cgaaatggcg gccagatcgt ggtacccacg
     7621 ggtgcgctga ttggcctcga cgccgtcacc gcggcggcgg tcgggaccat ccattcggtt
     7681 cggatggtaa cgcggaagcc gatcgccggg ctcgccggcg cgccttacat catcgagaac
     7741 aacatcgata tcgagcgaat taccgagccg ctcaggatat tcgatggtac ggcgcgggag
     7801 gcggcaaaag gatttccggc gaatctgaat gttgcagtcg cgctgtcgct ggcgggaata
     7861 ggccctgacc ggaccaaact ggaaatttgg gccgatccga cggtgacacg caacattcat
     7921 cgcattgagg tggaatcctg ctctgcccgc ttttccatgt cgatcgagaa tataccgtca
     7981 gaaaatccaa agacgggtcg gatcaccgcg ctttccgtca ttgcctatct gcgcaaacag
     8041 cgagcgtcgc tccgcgtcgg aacttgaatc acacgcaaat gccattcaac cgaatcggcg
     8101 cctcgcgtga ttgcacaacc gcatttggcc gttcgctgtt cggcggactg ctcaagtatg
     8161 gagcagcaag catctgattt cagtgctttt gttcgtgccg cggattcaga cttgcgaaca
     8221 gcttcaccgc gaaatcaagg agacacctat gatccagcag cggcgagtcc gctgagggca
     8281 aggcaaacta ccgcgactat acggttcgcc tcggtgacac cagcccggag gggatgctgg
     8341 aaaaagccaa atgggtattg gacgaaatgg agcggcgcat gagcgcattc tccagcgatt
     8401 ggcgtcaggc cacggcggtg cagctctaca ccgttcgcaa tgtgtttcct ttcctgcaga
     8461 gcgaactcgg caaacgcggt acgctgcgtc ctggtctgac ctggcacttc gatcgcccgc
     8521 ccgtggtcga tctggaatat gagatggact gccgccgcgt ccacatcgag cgcgtaatcg
     8581 aagtttgacg tttcgccctc ttcttgccaa tctgcattca gacatccgcg ccttatgcct
     8641 ctgagtgccg ggatcaaagc tcaacgcacc ttgcttgtgg caaagtttgg ggagcaacga
     8701 taggcaggtt cacaatgcaa atccgtgacg acgatctact gctggtaatc gacgtgcaga
     8761 acgatttttg cgccggcggc gcgcttgcgg tcgccgacgg cgatgccgtg gtgccgatcg
     8821 tcaatcgcct caccgagcgc ttcaatcatg tcgcgctgac ccaggattgg catccgtccg
     8881 gtcacagctc ctttgccaca tcgcatccgg gttctgctcc ctttcgaacc atccgcatgc
     8941 catatggcga gcaaacgctc tggcccgatc actgtatcca gggcgcgccg ggcgccgcct
     9001 ttcatccgca attggtgacg gagcgggcgg agttggtgat ccgcaaggga ttccgccccg
     9061 aaatcgattc ctattccgcg ttctatgaaa acgaccggcg gacgcctacc ggtttggctg
     9121 gctatctgcg cgagcgcggt ttgcggcgga tcttcctcgc cgggctcgcg accgactatt
     9181 gcgtgtatta ctccgctgtg gacgcgcgcc ggcttggctt cgacactgtg ctcatcgagg
     9241 cggcctgcag ggccatcgat ctcgcaggct cgctcgatgc cgcctgggcg gggatggcga
     9301 cggccggcgt gcagcgcgtc aacgatctca gctagcgcgt atctcgcaaa agcgcgtcct
     9361 cgttttggag catgtctgta tccggttggg tggcaattcc ggaaatgccg gctcgctgct
     9421 gtcgacgtcc tcggcggcga tggagcgatc gcttaagctc gggggtgccg gtctccgctt
     9481 tgtcgcttgc aaggtctcgg ctaaggaaac taatatcgag cgcgcgtcca ccctgtccat
     9541 gcggcccgga ggaatagctg atgcgtcgca ttctgctggc gctcgcaatg gcgcttacgg
     9601 ttattgcact gcccggcgtc acttttgcga tcagcgacga agagacggtg cgccttctta
     9661 agcacgacgc tgaccagggt aacgcgaatg cgcaaggtcg tctcggctat ttctacgaaa
     9721 ccggtcgcgg tggcctcgcc aaagacgatc gcgaggctgc acgcctctac aggctcgccg
     9781 ccgaccaggg caacgcctcc gcacgaaaca atctcggcgg gctttacagg gacggccgtg
     9841 gcggcctcac caaagacgac cgcgaggctc tgcgcctctt caagctcgcc gccgatcagg
     9901 gcaatgctta cggacaacta aatctcggcc tcttctaccg agagggccgc gggggtctca
     9961 gcaaggacga ccgcgaggct gcgcggctat tcaagctagc cgccgaccag acccaggccg
    10021 acgcgagagt taatctcggc tatttctacg aaaccggtca cggtggcctt gccaaggacg
    10081 accacgaggc tgtgcgcctt taccggctcg ccgcggatca gggcaacgcc acagcacaaa
    10141 gcaatcttgg cgtcttctat agagatggcc gcggtggcct caccaaggac gaccgcgagg
    10201 ctctgcgcct cttcaaactc gccgccgatc agggcaaccc ctacggtcaa aaccagctcg
    10261 gcggtttctt tcgagacggc gggggcggcc ttgccaagga cgaccgcgag gctgcgcggc
    10321 tgtttaagct ggccgccgac cagggcaacg ctagcgcgag agttaatctt ggttacttct
    10381 acgagaaagg ccgcggtgac cttgcccagg acgaccgtga ggctgcgcgg ctctacaagc
    10441 tcgccgccga ccagggtaac gccgccgggc aaacttatct cggtgttctc tacagggacg
    10501 gccgcggtgg tctcaccaag gacgagcgcg aagccgcgcg cctcttcaag ctggcctccg
    10561 accagaacta cgcctatggg cagcaaaatc ttggcatttt ctaccgagac ggtcgcggcg
    10621 gcctcaccat ggacgaccgt gaggctgcgc gcctccttag gctcgccgcc gaccaaggca
    10681 acgctaacgc gcgaacggcc ttgaaccaga tcactcagca accctcgcaa gaggtaagtt
    10741 cggctcctag tgcacattta aagcaagagg tagtcgaacc aagctcagca cctgccgcca
    10801 atttgccttc ggtgccggag cgccgcgtgg cacttgtcat tgggaattcc gcttatcaaa
    10861 ctgtgagcgg actccctaac acgacaaacg acgcgagagc gattgccgag gtactcaagg
    10921 ccgacggatt caccagtgtg cgaataattt tggatatgac ccgggcaagc atgattgcct
    10981 ccctcaatga ttttcaacgc gaagctgaca catcggattg ggccgtcgtc tattatgccg
    11041 gtcacgggct ggaggttagt ggcgcgaatt atctcgtgcc gatcgacgca aaactggggg
    11101 acgaccgaga tattcaggat gaggcggttt cagtggatcg aattctgagt gcggtcgaaa
    11161 acgcgaaaaa gctcaggctc gtcatgctcg acgcatgtcg cgataatcct tttctgaaga
    11221 ggatgcatcg gagcatcgcg acacgttcga taagccgtgg actagctgct atcgaaccca
    11281 tgggcgccac actgatcgtg tttgccgcaa aggatggtga gacggcggag gacggaactg
    11341 gcggccacag tccgtttgcg gcctcgctca tcaagcgatt gcagcagcca actattgaaa
    11401 tcaacaagct tttccgcctc gtaaccagtg acgttctaaa agctactggc aatcaacagc
    11461 ggccattcgt ctatggatcc cttccaggcg aggaagacta ttatttcagg ttgagatgaa
    11521 tttgcgcatg cgcatatcga ttcgatatca cgcggttcgc gatgtccggt tcggcattcc
    11581 gccggctgcg atgatttcag cactcaattg ctcgatcgtc gtgcggtgcc gcatggcggt
    11641 ctgccggatc agcctgaacg cgtcagattc ggaaagatca tgagcgcgca tgatttgcag
    11701 aaccgcggcg aacaccacac gccgggagcg gatgcggtcc tctaatcttt cgagatgcgc
    11761 ggtttcatca attcttcgct gggccgaatc gaacgcaacc accagcgcgg tgtagagtcc
    11821 tgccgagtgc agcggcttga tgagaaaaga tgcaggctgc aggtcaagca gccacttcaa
    11881 ccggctgggc gtctcggttc cgatcagtgc gatgatcggc gcgccggcct tccacgccgc
    11941 ggccagatca gacttgatgg gaatgctgtc cgcatccatg acgacgacgt cagcgttttc
    12001 gtccggcggc gccctagccg gatcatgttc ggcgatcgtc atgccgaggc ggctgaattg
    12061 gcggcgcacg atggagattt cacgctcgtc tctcatcacg atgagcgcgc gacggccgcg
    12121 gacggagaag ggcgatggtc ttgtcatttc acgatacgca ggtggcttga ccgttttgtc
    12181 attgcatcat tactcctttc aagcctcgtg ccaaccgccg tgagatcgag gttcgaaaga
    12241 taggggtcgg ggcgctccgg ggcgtccgct tcccagaaga tcttgaactg acacccgggc
    12301 acggagcgtg ccaaacgggg tgtcaggaag cagtgattat tgtcgggatc gacccaaacc
    12361 gggccttgcg gcgaatcgta ccgatgattt gccgcagcgc accggacggc tccgacgtcg
    12421 gcggagccgg cgcggcggat ggcgcgggca agcagcatga cgcagacata agtcgactgc
    12481 ccatcgacgg acgggttgct gtcggcgccg tgacgggcct tccaccgtgc gacaaaagcg
    12541 cgattttcgg gccgctcgat gctctcgaaa taagcagacg aggtgatgca tccgacggag
    12601 gccgccggtc caatcagttt gagctcgggc tcgcacagac tgcagctcag catcgggatc
    12661 gtcagtctcg cgcggaccat cgcctcgtgg agcgcgcgaa cgaagacgta gctggattcc
    12721 ccgacaagtg tgttgaacac agcaggcggc ttgcggtcga tgatttccct gacgatgtgg
    12781 tcgacggcgg tctcgccgag ctcaagcagg cgttcggcaa ggatgcggcc gcccgcgctt
    12841 gtcacgattt cacgaatgac ccggttggtc tcccaggtcc agacatagtt ggacccgata
    12901 cagaacacgt cgctggagac ttgatccagc atatgccgca ccagcgggac gacgttgtga
    12961 ttgggcgcgg cgccgacata aatgacgttg tcgctgctct caaagccttc atatcgcgct
    13021 ggatgccaga gcagccgttc ggtgcgttcg acaatgggaa tcacctgctt gcgtgacgcc
    13081 gacgtgtagc agccgacgat atggtcgact tgttcttcgc gaatgagatc gtcgcatgcc
    13141 gtatgataag cggcgacgac gccgccggga tcacgaagat gcggcgtgaa tgtgaaatcg
    13201 aactcgtcgc tgcgattgac ttcgtcgatg gccatcaacg cgcttttgcg catctcgccc
    13261 gccatcatgg cgtaaggtcc cgattgggaa aataccaggc cgagaggcac cgatggcttc
    13321 ttcataaagc agctcgaagg ttgcgtggcg aatgatcaca tcgcaagaat gtcgatctgt
    13381 caccttgtat catcaatctg cgctgcgcgt cgtttgtgct cgtccatgca taaaaaaggt
    13441 gccggcatga gattgacaag tccggtgacg ctaagacaca atcaatttgt acgtgaatgc
    13501 gtgcaaggcg aaagagttct cgcgcagccg atgacgtctg agtgaaaacc ttcgattttc
    13561 gttctgtaac gcaagactac gcgaccattg atagcagcgg cgtggatgcc attcggggcc
    13621 tgtttgggtc gcgggtggct ttttttgttt gtcttccaaa agacaagaat tgggtgaagc
    13681 atggtagtgc gccttccgac attggagcag attgacaacc taggatccga tttcggtctg
    13741 gttcttaacg ctgacgaagt tgcggccttt cagcaggcct tcaaaggacc gttggcctcc
    13801 tacggccggc tcgacgaatt ggtcccgccc gccttggcgc cggtggcgcc acgctcgccc
    13861 ggataccggc ctacggcatc cgagaatccg tacggcgcct ggtattggaa gacgaatatc
    13921 caaaccggcg cggaagggct gctgagcggc aagcaggtag cgatcaagga taatatctgc
    13981 gtctcaggtg tcccgatgat gaacggctcg gtgctgcttg aagggtatgt gccggagctc
    14041 gatgccacgg tggtgacgcg gattcttgca gcgggaggca ccattgcggg caaggcggct
    14101 tgcgaggatc tctgcttttc cggtgcaagc cacacctgtg cgagcggcgt gatccgcaat
    14161 ccgcataacc ccgcgcatag cgccggcggc tcatcgggtg gaagtgccgc gctggtggcg
    14221 gcgggtgagg tgccgatggc gctcggcggc gatcagggtg gatcgatccg tacgccgtca
    14281 agctggtgcg gcatttatgg cctgaagccg acctggggac ttgtgcccac caccggttcg
    14341 atgccgatca gttattccgt cgatcactgc ggtccgatgg gcgcaagcgt tgaagacgtc
    14401 gcccggcttt tgacggtcat cgccggccac gacggctggg atacgcggac gatttcagcg
    14461 cgcaccggcg actacatggc cgcactcggc aagcccgcca agggattgcg cgtcggaatc
    14521 ctgcgtgaag gatttggtca tcccgaaagc gatccggcgg tggacgccaa agtgcgcgcg
    14581 accatcgaag cattggcaaa gagcggcgtc gaagctgccg aaatctcggt gccctggcat
    14641 ctcgacgggc cgcatgtctg gagcgggatc atcctggagg gggcggccga gatgatgctg
    14701 aagggatatg gcgtcggcaa caacatccac ggctactatc cgctttccat gcaggaagcc
    14761 tttgcccgcg gcatgggaac gcggatcaac gacgtctcgc cgacggtgaa actggtgctg
    14821 atgctcggcg aatacatgca ccgcaattat cacgggcgtt atcattcaaa agcgcaaaat
    14881 ctgcgggtgc tgctgcgtcg cgcctatgac gaggcgctcg aaaaattcga cgtgctggcg
    14941 atgccgacga ttccgttcac ggcaacgcct ataccgccgg cggatgcgcc gctcggcacc
    15001 acgatcgacg tggcgctcaa catgcaggcc aacacctgtt ccttcgacgt ctccgggcat
    15061 cctgccttca cggtgccatg cggccgcgtc aatggattgc cggtcggcct gatgctggtc
    15121 ggccgccatt tcgaagaaac aaccctgata cggcttgctt cggcgatcga agcgggcggc
    15181 gactggaagc tgaactagaa cacgaggcaa cacttgatgc cgacgcgcga tctgcattac
    15241 tgcgaattgg tcgaaattgg gcaacgtatt cagaaacgcg aactgtctcc cgtggaggca
    15301 acgcaggccc aactcgatcg catcgcgcgt ctcgacggcc aattgaaaag ctacgcctat
    15361 gtcatggctt cgtcggcgct tgagcaggcg cacgccgccg agagggaaat cgggagcggc
    15421 aaggtgaggg gaccgcttca tggggtgcct atcgccgtca aggacctgtg ctggaccaag
    15481 gacgcaccga ccgcggcggg catgaggatt tatcgcgata accgccccac ccaggatgcg
    15541 acggtcgtta ccaggctcaa ggaggccggc gcggtcattc tcggcaagct gcaactgacc
    15601 gaaggcgctt acgccgacca tcatccggac atcgatccgc cgcgcaatcc ctgggatgca
    15661 acgctctggt cgggcgcatc ctcaagcggg tcgggcgtcg cgaccgccgc ggggctttgt
    15721 tacggctcgc tcggatccga taccggcggc tcgatccgct ttccgtcggc cgccaacggt
    15781 gtgaccggcc tgaaaccgac ctggggacgg gtcagccgct acggcgtttt cgaactcgcc
    15841 gcgacgctgg atcatatcgg accgatggca cgaagtgcgg tggattgcgg cgcgatactg
    15901 ggcgccatcg caggcgccga tccaaacgat ccgaccgccg tgctggagcc ggt
//
LOCUS       DASWGM010000114         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2227251,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000114 DASWGM010000000
VERSION     DASWGM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227251"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /gene="priA"
                     /locus_tag="VGA15_03460"
     CDS             complement(<1..1543)
                     /gene="priA"
                     /locus_tag="VGA15_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX9586785.1"
                     /translation="MDHSTRASTLSASSARVVDVLVPVALNQAYSYRVPRGMELRPGD
                     VVCVPLGPREVVAVVWAENANPDPRLHNRLKDVGEKLDVPPLKDELRSLVDWVANYTL
                     SARGMVLRMTLRMGEHLGPERVRMGVRLIGASPQRMTPARRRLIDILSDGLLHAKSEA
                     AKEAGVSTGVIDGLVDEGTLTTEAMPRAQAPPAPDPSFSAPDFSRLQRTAVDAMRALA
                     ANGSFHVALLDGVTGSGKTEVYFEAIAEIIRRGKQSLILMPEIALTGQFLDRFAHRFG
                     VRPLEWHSELTPRTRQRNWAAIAAGEAPVVVGARSALFLPYADLGLIVVDEEHDQAYK
                     QDEGAHYHARDMAVVRAHIAKIPIVLASATPSVETEVNARKGRYQRVVLPSRFGGQHM
                     PHIEAIDLRREAPVRGRFISPRLAEHIQIAIERREQALLFLNRRGYAPLTLCRACGHR
                     FACTICDAWLVDHRFRQRLVCHHCGFSMPRPHVCPHCAAEQSLVAVGPGVERLQEEAA
                     QLFPDA"
     gene            1701..2672
                     /locus_tag="VGA15_03465"
     CDS             1701..2672
                     /locus_tag="VGA15_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX9586786.1"
                     /translation="MAKPTPAVPPIELDCAADDIALQMTRWLSHLRAERRLSPKTVEA
                     YARDLRQCLVFLCEHWGTRVTLARFAGLEASDVRAFMAMRRADEIGSRSLMRALAGLR
                     SFGRFLEREGNGKVGALSAIRAPKVGKSLPKPIQMAAAKRLTDADERAGETRDPWIWA
                     RDAAVMALLYGSGLRISEALGLKRRDVPLPGVGDVLIVTGKGNKTRMVPVLQNVLTLI
                     QDYVAMCPHPLPAQGPIFVGARGGPLKARIIQLTMERMRGALGLPDSATPHALRHSFA
                     THLLTRGGDLRAIQELLGHASLSTTQIYTGIDTERLLEVYKTAHPRA"
     gene            2780..3370
                     /locus_tag="VGA15_03470"
     CDS             2780..3370
                     /locus_tag="VGA15_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX9586787.1"
                     /translation="MSAHESMEQAEHTKEAAGENRKIALLIAVIALCLALSETLGKGA
                     QTESISKNVEASNLWAFFQAKTIRRTVVQATSDQAKLSLGVIGDDAAKAALQKQIDEW
                     QKTAARYRSEPETGEGSEQLAERAKHAEEERDLATAKYHHFELASAAFQIGIVLASAT
                     IITGILALAWISGLLALAGIAFTALGVFVPHLLHLP"
     gene            complement(3497..4027)
                     /locus_tag="VGA15_03475"
     CDS             complement(3497..4027)
                     /locus_tag="VGA15_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586788.1"
                     /translation="MMRRLLQPFWVLLAVIFLIEAWLWDHLEPIVARVVALIPLRAFK
                     QWLAERVDTLSPAMTLIVFIVPVIPLFPLKLVGFWLLTHEYWFSAITTIIFAKFLGVG
                     VAAFVFDVTRHKLLEMAWFETLYEFVISLRAKAAALVDPIKLRIMEALRGGGGGWSSR
                     MLRLIQRFRKSVHEAR"
     gene            complement(4066..>4942)
                     /locus_tag="VGA15_03480"
     CDS             complement(4066..>4942)
                     /locus_tag="VGA15_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9586789.1"
                     /translation="LLIVGAGVIGLELGSVWHRLGAKVTVVEFLDRILPGMDGEVARQ
                     FQRILEKQGFAFKLSAKVTGIDTTGNTLVAKIEPAAGGPSEKLEADVVLVCIGRVPYT
                     EGLGLKEAGVALDNRGRVQIDPHFATSVKGVYAIGDVVAGPMLAHKAEDEGVAVAEIL
                     AGQAGHVNYDVIPGVVYTTPEVSSVGKTEEELKQAGVAYTSGKFPFTANGRSKVNQTT
                     DGFVKVLADAKSDRVLGVHIVGREAGEMIHEAAVLMEFGGSAEDLARTCHAHPTRSEA
                     IKEAALAVGKRTIHM"
BASE COUNT          950 a         1628 c         1534 g          830 t
ORIGIN      
        1 gcgcatccgg aaacaactga gctgcttcct cctgcaagcg ctcgacgccg gggccgaccg
       61 caacaagcga ttgctcggcc gcgcaatgcg ggcagacatg cggacgcggc atcgagaagc
      121 cgcagtgatg gcagaccaga cgctggcgaa accggtgatc gaccagccag gcatcgcaga
      181 tcgtgcaggc aaagcgatgg ccgcaggccc ggcataaagt cagcggcgca tagccgcggc
      241 gattgaggaa cagcagcgcc tgctcgcgcc gctcgatcgc gatctggatg tgctccgcca
      301 gccgcggcga gatgaagcgg ccccgcactg gcgcttcgcg acgcagatcg atcgcctcga
      361 tgtgcggcat gtgctggccg ccgaaccgcg acggcagcac cacgcgctga tagcggccct
      421 tgcgcgcatt gacctcggtc tcgaccgacg gcgttgcgga agccagtacg atcgggatct
      481 tcgcgatatg cgcgcgcacc accgccatgt cgcgggcgtg gtaatgcgcg ccctcgtcct
      541 gcttgtaggc ctggtcgtgc tcctcatcga cgacgatcaa tccgagatcg gcatagggca
      601 ggaacagcgc cgaccgcgcg ccgaccacga ccggcgcttc gcccgccgcg atcgcggccc
      661 agttgcgctg cctggtgcgc ggggtcagct cggaatgcca ttccagcggc cgcacgccga
      721 agcggtgcgc aaagcggtcg aggaactgtc cggtcagcgc gatctccggc atcaggatca
      781 gcgactgctt gccgcggcgg ataatttccg caatggcttc gaaatagact tcggtcttgc
      841 ccgaaccggt gacgccatcg agcagcgcga cgtgaaagct gccgttggcg gcgagcgccc
      901 gcatggcgtc caccgcggtg cgctgcaggc gggaaaagtc gggcgcagag aacgaaggat
      961 cgggcgcggg cggcgcttgg gcgcgcggca tggcctccgt cgtcagcgtg ccctcatcga
     1021 ccaggccgtc gatcacgccg gtgctgacgc cggcttcctt ggcggcctcg gatttggcgt
     1081 gcagcaaacc atccgacaaa atatcgatca gccgccgccg tgccggcgtc atgcgttgcg
     1141 ggctggcgcc gatcagccgc acgcccatcc gcacccgctc gggtccgaga tgttcgccca
     1201 tccgcaacgt catccgcagc accatgccgc gcgccgacaa tgtgtagttg gcgacccagt
     1261 cgaccaggct gcgtagctcg tccttgagcg gcggcacgtc gagcttttca ccgacgtcct
     1321 tgaggcgatt gtgcaggcgt ggatcgggat tggcgttttc cgcccacacc acggcgacca
     1381 cctcgcgagg ccccagtggc acgcagacca catcacccgg cctgagctcc atgccgcgcg
     1441 gtacccggta ggaataggcc tgattgagcg caaccggcac cagcacgtcg acaacgcgcg
     1501 ccgacgatgc ggatagggtg ctggcacgcg tggaatggtc cattgcagaa tcagactcga
     1561 agtgaaaggc cgccgctaac ttagggccgc gcgcgggccg ggaccagtgt ccggccgctg
     1621 ccggcttcgc aagttagcga agggtaaacc aaagaagtgc gatatgatcg ccgggaatca
     1681 tcgcgcccga agactggctc atggcaaaac caactcctgc tgttccgccg atcgaactcg
     1741 actgcgccgc tgacgacatc gcgcttcaga tgacgcgctg gctgtcgcat ctgcgcgccg
     1801 agcgacggct gtcgccgaag actgttgagg cctatgcccg cgacctgcgc cagtgtctgg
     1861 tctttctctg cgagcactgg ggcacccggg taacgcttgc gcggtttgct gggctcgagg
     1921 ccagcgacgt cagggccttc atggcaatgc gacgcgccga cgaaatcggc agccgttcct
     1981 tgatgcgggc gctggcaggt ctgcgctcgt tcggccgctt cctcgaacgc gaaggtaacg
     2041 gcaaggtcgg cgcgctatcg gcgatccgcg cgccaaaagt cggaaagagt ctgccaaagc
     2101 cgatccaaat ggcggcggcc aaacgcctga ccgacgccga tgagcgcgcc ggcgagaccc
     2161 gcgatccctg gatctgggcg cgcgatgccg ccgtgatggc gctgttgtat ggctcgggcc
     2221 tgcgcatctc ggaagcgctc ggcctgaaac ggcgcgacgt gccgttgccc ggtgtgggcg
     2281 acgtgctgat cgtgaccggc aaaggcaaca agacccgcat ggtgccggta ctgcagaatg
     2341 tgctgacgct gatccaggac tatgtggcga tgtgcccgca tccgctgccg gcgcaaggac
     2401 cgatctttgt cggcgcgaga ggcggcccgc tcaaggcccg aatcatccag ctcaccatgg
     2461 aacggatgcg cggcgcgctc ggcctgcccg acagtgcgac gccgcacgcg ctacggcatt
     2521 ctttcgcaac ccatcttctc acccgtggcg gcgatctgcg cgcgatccag gagctgctcg
     2581 gccacgcttc gctctcgacc acacagattt ataccggcat cgataccgaa cggctgctgg
     2641 aagtctacaa aacggcgcat ccccgcgcct gagatcgctt ggctttcagt cctgacacgt
     2701 ttccgtcaca tccgcttgcc acttgcgcaa tcggcgttgt tcggtcaatc gtggatacca
     2761 gtcgaccgcg gagggaatca tgagcgcaca tgaaagcatg gaacaggcgg agcacaccaa
     2821 ggaagccgcc ggcgagaaca ggaagatcgc gctgctgatt gccgtgatcg cactttgcct
     2881 tgcgctgtcg gagacgctgg gcaagggcgc ccagaccgaa tcgatcagca agaacgtcga
     2941 agcctcgaat ctctgggcgt tctttcaggc caagacgatc cgccgcaccg tggtccaggc
     3001 gacgtcggac caggccaaac taagcctcgg cgtcatcggc gacgatgccg ccaaggcggc
     3061 gctgcaaaaa cagatcgacg agtggcagaa gaccgcggcg cgctaccgct cggagccgga
     3121 aaccggtgaa gggtcggaac aattggccga gcgcgccaag cacgccgagg aagagcgcga
     3181 cctggcgacg gcaaagtatc atcacttcga actcgcctcc gccgcgtttc agatcggcat
     3241 cgtgctggct tcggcgacga ttataaccgg catacttgcg ctggcctgga tctcgggcct
     3301 gctggctttg gcaggtatcg cattcaccgc gctcggcgtg ttcgtgccgc atctgctgca
     3361 tctgccgtag cttgactcgc ctctccgcaa ccccgtcatc ccaaccctct ccccaagggg
     3421 taagggaatg caccgcggtt ggcgcgaatg tgttggcaag tcttgaacag tcccggcaac
     3481 cgaagcttcg ggaggcctac cgcgcttcgt gcacgctctt gcgaaagcgc tggatcagcc
     3541 gcagcatccg cgacgaccag ccgccacctc cgccgcgcaa ggcttccatg atccgcagtt
     3601 taatgggatc gaccagcgcg gcggcttttg cgcgcaacga aatgacgaat tcatagagcg
     3661 tttcgaacca cgccatctcc agcaatttat ggcgcgtgac gtcgaaaaca aaggccgcga
     3721 cgccgacgcc aaggaatttt gcaaagatga tggtcgtgat cgcactgaac caatactcgt
     3781 gggtgagcag ccagaagccg accagcttca gcggaaacag cggaatgacc ggcacgatga
     3841 aaacgatcag cgtcatcgcc ggcgacagcg tatcgacgcg ctcggcgagc cactgcttga
     3901 acgcgcgcag cgggatcagc gccaccaccc gcgccacgat cggctcaagg tgatcccaca
     3961 gccaggcctc gatcaggaag atgaccgcaa gcagaaccca gaacggctgt agaaggcggc
     4021 gcatcatcaa tgttgtcccc gcccgactgc aagccgcggc gtgaatcaca tatggatcgt
     4081 gcgcttgccg actgcaagcg cagcttcctt gatggcctcc gaacgggtcg gatgcgcatg
     4141 acaggtgcgc gccagatcct cggcggaccc gccaaactcc atcagcaccg cggcttcgtg
     4201 gatcatttcg ccggcctcgc ggccgacaat atgcacgcca agcaccctat cgctcttcgc
     4261 atctgcgaga accttcacga atccgtcagt ggtttgattg accttggagc ggccattggc
     4321 ggtgaatgga aatttacccg acgtataggc cacgcccgcc tgcttcaact cttcctcggt
     4381 cttgccgacc gacgacactt ccggcgtggt atacacaaca cctgggataa cgtcgtagtt
     4441 cacatgcccg gcctggcctg cgagaatttc cgcaaccgcg acgccttcgt cctcggcctt
     4501 gtgggcgagc atcggacctg ccaccacatc gccgatcgcg tacacaccct tcacgctggt
     4561 cgcgaaatgc ggatcgatct gcacacggcc acgattgtcc agcgcgacac cggcttcctt
     4621 caggccgagg ccttcggtgt agggcacgcg gccgatgcag accagcacca cgtcggcttc
     4681 cagcttttcc gacggcccgc ccgcggccgg ttcgatcttc gcgacaagcg tgttgcctgt
     4741 ggtgtcaatg ccggtgactt ttgcactgag cttgaacgca aaaccctgtt tttcaagaat
     4801 gcgctggaat tgtcttgcga cttcgccatc catgcccggc aggatgcggt cgagaaactc
     4861 caccacggtg acctttgcgc cgagccggtg ccacaccgag ccgagttcga gcccgatcac
     4921 gccggcaccg acgatcaata at
//
LOCUS       DASWGM010000115         5731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1254767,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000115 DASWGM010000000
VERSION     DASWGM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254767"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(514..642)
                     /locus_tag="VGA15_03485"
                     /pseudo
     CDS             complement(514..642)
                     /locus_tag="VGA15_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013761690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(695..1741)
                     /locus_tag="VGA15_03490"
     CDS             complement(695..1741)
                     /locus_tag="VGA15_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018857309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9586790.1"
                     /translation="MSEEINHHRRRFLGAAAMTIAAAELVMIGSADAQPSKTKPADVP
                     PIKPGTNTSFGSLKQIDAGVLNVGYAEAGPADGPVVILLHGWPYDIHSYVDVTPLLAS
                     AGYRVIVPYLRGYGTTRFLSSGTFRNGQQAAVAVDVIALMDALKIEKAIVAGYDWGAR
                     TADIVAALWPQRCKALVSVSGYLITNREANKRPMPPQVEWGWWYQYYFATENGRAGYD
                     VYRHDFAKLIWKTASPNWGFDDATFDRTVASFNNPDHVSIVIHNYRWRISLAEGEPQY
                     DDLEQRLDAMPVIAVPTITIASDFDGAAADGTAYAKQFSGKYSHRILKGIGHNVPQEA
                     PQAFAAAVVEVDGY"
     gene            complement(1884..2861)
                     /locus_tag="VGA15_03495"
                     /pseudo
     CDS             complement(1884..2861)
                     /locus_tag="VGA15_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(3312..4646)
                     /locus_tag="VGA15_03500"
     CDS             complement(3312..4646)
                     /locus_tag="VGA15_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX9586791.1"
                     /translation="MSATSLSPTRRGFLAASAAAGAIGLLPAHLAAAAAAGDARPSQP
                     SNQGDSPMGTTDDNAIRPFRINVPEEALIDLRRRIAATRWPDRETVADRSQGVPLATI
                     QPLVRYWGTEYDWRKAEAKLNALPQFVTEIDGLDIQFVHVRSRHPNSMPLIMTHGWPG
                     SIFELLKVVGPLTDPTAHGGRAEDAFDLVLPSMPGYGFSGKPQSTGWGPDHIARAWAE
                     LMRRLGYKRYVSQGGDWGSVISDVMARQAPAGLLGIHVNMPATVPPEIAKALSCGEPA
                     PAGLSPEEKAAYEQMDALYKKGSGYALMMVTRPQTLGYSLADSPVGLAAWFYDKFADW
                     TYTGGQPEKELTRDEMLDDITLYWVTNTGTSSARLYWENNANNFNAVDINIPAAITVF
                     PGEIYRAPRSWAERSYHKLIYYHAVDKGGHFAAWEQPELFAAEIRAAFRPLR"
     gene            complement(4695..4877)
                     /locus_tag="VGA15_03505"
     CDS             complement(4695..4877)
                     /locus_tag="VGA15_03505"
                     /inference="COORDINATES: protein motif:HMM:NF039665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6130 family protein"
                     /protein_id="HEX9586792.1"
                     /translation="MFGSAALDISPRSGQVHVTVDAAPWYWADASGEPLIIQGLPPVH
                     PEKRGRGHRRQAHRRG"
     gene            complement(5423..5650)
                     /locus_tag="VGA15_03510"
     CDS             complement(5423..5650)
                     /locus_tag="VGA15_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586793.1"
                     /translation="MSEELNHHRRRFLGTAVMTIAAAELVMIGSADAQSSKINLADAT
                     TIKPGTNTSFGALKQIDAGVLNVGYAHCPLR"
BASE COUNT         1096 a         1710 c         1783 g         1142 t
ORIGIN      
        1 gcgggcggcg gcgtcggcgg ccgcgtcgga ttgttccgcg acccgccgga gctgaagcag
       61 gcgatcgagc agctgcacca ggcgcaggcc gaagccaacc tcaaatgctt tgaatgaggc
      121 ggcggcccag tctctgtact cataagcgac gcgaccttga ccgacgccgg atgccgtcaa
      181 gttccgctta tgaccctggc cgtgtaaaaa cgcgattgcg ggccgtcgcc gtgaaatctc
      241 cgatcgctgc gggatcgaca gaagaacgaa tgattcaaag ggccgctcta cggagcaacg
      301 ggattactcg ttggtgttac ggcacatcgt tttcacacgg ccaggaccca cagcagacat
      361 tcccaacgaa gctgtaatcg ccgctaacgg tccttccaaa gcgtttgtcc gaaccgttac
      421 gatgccttct ttcagcctgg gggcgggcat gaagcgacgt gagttcatca acctgattgg
      481 cggtgctacg gcgtggccgg tggcggcgcg ggcgaaccgg tcggtctgcg cgagaccgtg
      541 tgatgcgccg gaatagacct tgagctgagc gctcttgacg atcttggcgg ccttttcggc
      601 ggaagccgcg acggggacga tctgatcgtc gtcgccatgg atgtcctgcg gactgtgtat
      661 gtcagtccgc agggcctacg caggctaacg ccgatcaata accgtcgact tcgacgacag
      721 ctgcggcgaa ggcttgaggg gcttcttgag gcacattgtg accgatgccc ttgaggattc
      781 ggtgcgaata tttgcccgag aactgcttgg cataggctgt tccgtccgcg gccgcgccat
      841 cgaaatcgct ggcgatggtg atcgtcggca ccgcgatcac gggcattgcg tccagtcgct
      901 gttcgagatc gtcgtattgc ggctcgcctt cagccaggct gatgcgccag cggtaattat
      961 ggatcacgat gctgacgtga tccgggttgt tgaaggatgc tacggtgcga tcgaacgtcg
     1021 cgtcatcgaa gccccagttc ggcgaggcgg tcttccagat gagcttcgcg aagtcgtgcc
     1081 ggtatacgtc gtagccggcc cggccgtttt ccgtggcaaa atagtactga taccaccatc
     1141 cccattcgac ttgtggcggc atcggccgtt tgttcgcttc acgattggtg atcagataac
     1201 cgctcacgga gacgagggcc ttgcagcgct gcggccagag cgccgcgacg atgtcggccg
     1261 tccgcgctcc ccaatcatac ccggcgacga tcgccttctc gatcttgagc gcatccatca
     1321 aagcgatgac atcgacagcg accgccgctt gctggccatt ccggaacgtt ccgcttgaaa
     1381 gaaagcgcgt cgtgccatag ccgcgcagat acgggacgat cacccggtag cccgccgacg
     1441 ccagcaaagg agtgacgtcg acatagctgt gaatgtcgta gggccagccg tgcagaagaa
     1501 tgaccacagg accatcggcg gggccggctt cggcgtagcc gacattgagg acgccggcat
     1561 cgatctgctt cagcgagccg aacgacgtgt tcgtccctgg cttgatcggg ggcacatctg
     1621 ccggtttggt cttgctgggt tgtgcgtccg cagaaccgat catgacgagc tcggcggcgg
     1681 caatggtcat ggccgcagcg cctaaaaagc ggcggcgatg atggttgatc tcttcggaca
     1741 ttttgatcgt agccatcggt gactctcctt ggttcgatga ctgtgcttca ccgcattatg
     1801 gtggcagcga tgaaggtgac tcggttcgac ccgatcgagg cccgccaggg cgcggtgatc
     1861 gggtgaatag cctctgccgt ggatcagccg aacgtgaacg caaaagccgc cacgcccgaa
     1921 tcgagaaact cgatctcgaa ctgtcgatca gcgatcggct tcggttgtcg gatcagctga
     1981 tacagccgct gttcggcagc cgtgccgttg ccttggtcgt cgacgtcaat tccgtgagca
     2041 gcgcccggcg gctgtccatc gatgagcacg cgaaagcgca cggaagctcc tcgcgctgcc
     2101 ggtcccatga cgagatgaag atcgcgggcg tgaaagcggt acgcgatccg cccgttggcc
     2161 ttgttcagca cggtggcctg tttcgccacc gtccaatcac ccgacagggc ccaattgttg
     2221 agtctcaacc gcgcgggggc agcatagacg cgacgcttgt ccaatactgc accgccggga
     2281 gacgcaaagt tctcggtgcg cgcatagccg acatagtttt ccggcgactg caagctaacc
     2341 caatcggcgg cagcttccgc accacgggca tcgaccgaaa ccagttcatg accgatgccg
     2401 ccgcttccgg cctcggccag caattgttga atgatcgttt ccgactgtgc gtattcgccc
     2461 tcgccgaatt gatgatggcg aatatgcccc tgcgcatcga cgaaatagag ggccggccaa
     2521 tattggttct tgaaagcacg ccatatcgca taatcgttat cgatcgcgat cggataatcg
     2581 accctcatgt ccttcgcggc gcggcgaacg ttatcgacgt ttttctcgaa tgcgaactca
     2641 ggcgcgtgca cgccgatcac caccaatcct tgatccttgt atttctcggc ccaggcgcgg
     2701 acatagggaa gcgagcgcag ccaattgatg caggtgtagg tccagaagtc gatgaggacg
     2761 acttttccgc gcaggccggc cgccgtcaat ggcggcgaat tgagccactc ggtcgcgctg
     2821 ccgagagaag gcaattcacc ttcgatgggt aatcgaaccg ctgcgggcgt catctgctgc
     2881 accacgcttt tgtccccggc taaggcctcg atcggtgccc cgatgaaagc aagccagctc
     2941 ttgctcgccg cgacactcgc ccccgcaatc gcggcaatgg tcatggccga agtgccaaaa
     3001 aagcggcggc gatgacggtt gatttcttcg gacatgcatc tctctcccat cggactccaa
     3061 tcggattcga atcgatgttg ccgcgtgtgg cgtgggaaga cggctgtgcc caacatgcgc
     3121 gccgtccacc tcactccacc tagtggtgcg aatttaacat ttcgattctc cggccacagg
     3181 cgtcattgcg aggagcgcga cagcgcgacg aagcaatctc ccgccggcat catgcattgc
     3241 ttgcacgaga ttgcttcgct tcgctcgcaa tgacaacgaa tgcatgggtt cgcagtgtta
     3301 gactcggtcc actagcgcag cggtctgaac gccgcgcgga tctcggcagc gaagagttcc
     3361 ggctgttccc acgccgcgaa gtgaccgccc ttgtcgaccg cgtggtaata aatgagcttg
     3421 tgataactcc gctcggccca acttcgtggc gcgcgataga tctcgccggg aaagactgtg
     3481 atggcagcgg gaatgttgat gtccaccgca ttgaagttgt tcgcgttatt ctcccagtag
     3541 agccgcgcgg aagatgtacc ggtgtttgtg acccagtaga gcgtgatgtc gtcgagcatc
     3601 tcgtcccgcg taagttcttt ctcgggctgg ccgccggtgt aagtccaatc ggcgaacttg
     3661 tcatagaacc acgccgcgag cccgacaggc gaatccgcca ggctgtagcc cagagtttgc
     3721 gggcgcgtca ccatcatgag ggcatagcca gagcccttct tgtacaaggc atccatttgt
     3781 tcgtacgcag ccttttcctc gggggacagc cctgccgggg ccggctcgcc acagctgaga
     3841 gcctttgcga tttccggcgg cacggtcgcg ggcatattga cgtggatgcc aagcagtccc
     3901 gccggcgcct gacgcgccat cacgtccgag atgaccgagc cccaatcgcc accctgagac
     3961 acgtagcgct tgtatcccag acgcctcatc agctccgccc aggcgcgagc gatgtggtcg
     4021 ggaccccagc ctgtgctctg cggcttgccc gagaagccat aaccgggcat cgaaggcaga
     4081 acgagatcga aggcatcctc ggcgcgtccc ccatgggccg tgggatcggt gagcggacca
     4141 acgaccttca ggagttcgaa gattgagccc ggccagccat gggtcatgat cagcggcatg
     4201 gagttcggat gacgagagcg gacgtgcacg aactggatgt cgagcccgtc gatctccgtc
     4261 acaaactgcg gcaaggcgtt cagcttcgcc tccgccttcc gccagtcgta ctccgtcccc
     4321 cagtagcgta cgaggggctg gatcgtcgcc agcggcacgc cctgggatcg atcagcgacc
     4381 gtctcccggt cgggccaccg tgtcgccgcg atgcgtcggc ggaggtcgat gagtgcctct
     4441 tccggaacgt tgatgcggaa ggggcggatc gcgttgtcgt cggtcgtacc catcggtgaa
     4501 tctccttggt tcgatggttg gctgggacgt gcgtcgcccg ctgcggccgc tgcggccaga
     4561 tgcgcgggaa gcaggccgat ggcacccgcg gctgctgagg cggcaagaaa gccgcgccgg
     4621 gtcggcgata acgatgtcgc ggacatgacc attctccttg gtggaaatgc gagcttggcc
     4681 gtgggtgaat gggttcaccc acgacgatga gcctgccgtc gatgcccgcg gccgcgcttt
     4741 tcaggatgaa ccggagggag gccttggata atcagcggct cgccgctggc gtcggcccaa
     4801 taccatggcg cggcgtccac agtgacgtgc acctgtccgc tacgtggcga gatatcgagg
     4861 gcggcagagc cgaacatcgg gtgccatccg ccaggttttc agatcggtat tggataacga
     4921 cgaggcctcc cgctagtttc gcagagcgag aaaaaaaccg caagttgcgg agcgagcgcc
     4981 ccgcggacgc atgtgacgga ggtgagagta gcgcctatag aatggcggtc gaacgcgcgc
     5041 tgctcgcctg tctgcccggc gtgccgctgt ggctgatttg cagttcgcgc agctcgcgcg
     5101 aactgatttg gaacagcggc gcacgtcccg ccgtcccgag gcgatctaca acaacaggaa
     5161 acatggtcgg gcggctctgg gcgaggctca caaggagtgg cgtgcagtcg ctatccctac
     5221 gaaaagactg cctcctcttt cttgggcgtg ctctgcttgg ccgccagcat tgactgggtc
     5281 aacaggggag cggctctcgt accataggat gaacggcgtt tcaggcgcgc aacattgagc
     5341 tgcgcttaat gggagctgat cgatcccctt ttctcgttgt tcttctgcta ggctcgtgag
     5401 ccgttagaca ccgcaagcgc tgttatcgga gtgggcaatg tgcgtatcca acattcagga
     5461 cgccggcatc gatctgcttc agtgcgccga atgacgtgtt cgtccccggc ttaatcgtgg
     5521 tcgcatctgc caggtttatc ttgctagatt gtgcgtccgc agaaccgatc atgacgagct
     5581 cggcggcagc aatggtcatg acagcagtgc ccaaaaaacg gcggcgatga tggttgagtt
     5641 cttcggacat ttatctctct ccacttggga aatagcctgc ccgataaaca tgggcgagaa
     5701 tctgtccgat gcagcgcgcg ctaagcaata g
//
LOCUS       DASWGM010000116         7308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2367578,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000116 DASWGM010000000
VERSION     DASWGM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367578"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VGA15_03515"
     CDS             complement(<1..1074)
                     /locus_tag="VGA15_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9586794.1"
                     /translation="MAVTQAIPMTRHPYADGSYKKMLIDGKWIDAASGKRFETHNPAT
                     GELLATVAEGDAEDINRAVAAARRAFEGPWSKVKPYERQALLLKLADLVEKNFDELST
                     LDTLDMGAPISRTRGTKQRVLGMLRYYAGQATALHGETIENSLPGDIFSYTLKEPVGV
                     VGAIIPWNGPLGASVWKIGPAIATGCTVILKPAEEAPLTSLRLAELAMEAGIPPGVVN
                     VVPGYGETAGAALASHPGVDKVAFTGSHVTGQSIVKASAGNLKRVSLELGGKSPDIVF
                     ADADLDAAVPGAAMAVFANSGQICSAGTRLFVEHQVYDEFVGRVAEYGKKLQVGNGLD
                     PNTQIGPLVSEQQLDRVTGYLDVG"
     gene            1311..2162
                     /locus_tag="VGA15_03520"
     CDS             1311..2162
                     /locus_tag="VGA15_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9586795.1"
                     /translation="MPHAAMSANNVAVVTGGASGLGLAAAIRFARFGMKVCIADLDSN
                     RLAEAATKLSSVAPGGATDVMVTAVDVSRIEDVSELEIAVRERFGGTDILMNNAGIQP
                     GSEMFGPLQNWRRIIGVNLWGVIHGSQVFAPSMIERGRPGLIINTGSKQGITTPPGDP
                     AYNVSKAGVKAFTEALQHELRNTAGGRISAHLLIPGFVFTGLTAKGRTEKPAGAWTPA
                     QTIDFMIERLDADDFYILCPDNDVPRRLDERRILWAAGDIVENRPALSRWHPDYAEAF
                     AAFVKDS"
     gene            complement(2468..2848)
                     /locus_tag="VGA15_03525"
     CDS             complement(2468..2848)
                     /locus_tag="VGA15_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586796.1"
                     /translation="MEIDTQALTTCEVAADGGAISLGFVDSTGTPATIRLSLNQVGAL
                     AMTLPSLLDKALQTRFGDQSLRYAYPLASWVVEQSTDPTQGMVTLRTVDGFSVCFSIP
                     RQQQSELGEALVAQPAPKATLRAH"
     gene            3086..4432
                     /locus_tag="VGA15_03530"
                     /pseudo
     CDS             3086..4432
                     /locus_tag="VGA15_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            4509..5075
                     /locus_tag="VGA15_03535"
     CDS             4509..5075
                     /locus_tag="VGA15_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2478 domain-containing protein"
                     /protein_id="HEX9586797.1"
                     /translation="MFDSQCDLAALVYEQDQNPDEVLRQFASDLNASGYRAVGLVQLG
                     HHCLDAPKLSAMLVHTGEELQLFQNLGACATGCRLDVGQLLDAGSQVASAIDQGADLV
                     IVNRFGRQEREGKGLSFLVERALSADVPVVIAVPSHRFADWIRFADGMSVKLRCDRAA
                     LDAWWQTVSARHAGLVRQDHQTVCEVFK"
     gene            complement(5095..5301)
                     /locus_tag="VGA15_03540"
     CDS             complement(5095..5301)
                     /locus_tag="VGA15_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586798.1"
                     /translation="MSKQSMREEAERLIRETMERKAIVVKQGNTRIEATCGKCGAPNR
                     VSAAKGATRVKFACKECGHKQETL"
     gene            complement(5477..5893)
                     /locus_tag="VGA15_03545"
     CDS             complement(5477..5893)
                     /locus_tag="VGA15_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX9586799.1"
                     /translation="MARHIVARTTDIPPGGNKVVAVDGRDIVVFHVNGEFFALLNRCP
                     HEGAPLDKAACVARLTSPEPGVYQRSRVGELLRCAWHGWEFDMRNGQSYFDPARVKIR
                     TYPVVIEDGETLAKGPYLAETFPVHIEDSYVIVETT"
     gene            complement(5915..7027)
                     /locus_tag="VGA15_03550"
     CDS             complement(5915..7027)
                     /locus_tag="VGA15_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9586800.1"
                     /translation="MSDVIDRPLLEAEAPSKDRLRIIDCDVHPSLHARSDLNPFLSKR
                     WQQHMKTFGDHLRTPYIGTTPYPRSSPLIARRDAWPPTGGPPGSDLAFMRKQHLDPLD
                     IEFGILQVLDMFIFSQQNLEFGAAIQRAVNDWQLAHWTSREPRLKAAILVGQDDTEAA
                     VAEIDRCAKIGQYVQINVCPRANEPLGRRRYWPIYARAQELDLPLGIHVGGYGGHAPT
                     GGGWPSYYVEEHQSNAHSVAAQLSSLVIEGVPERFPRLKIVFIEGGLGWIPATTWRMD
                     QHFERFRDEVPHLKRRPSEYVTQHFWFTTQPIDEPDEAKHLRSLIEWIGVDRLLFSSD
                     YPHWDFDDPRFAFKTPLTEAERAKIFNGNARALYKL"
     gene            complement(7051..>7308)
                     /locus_tag="VGA15_03555"
     CDS             complement(7051..>7308)
                     /locus_tag="VGA15_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9586801.1"
                     /translation="REPAAIMRDHIRLTTQPFDAPPDAAGVADIIDMIGSDKMFLFAS
                     DYPHWQFDGDDAIPPHLPASLVSRMCADNPLETFPRLSLAA"
BASE COUNT         1400 a         2257 c         2283 g         1368 t
ORIGIN      
        1 gccgacatcg agatagccgg tgacgcgatc cagttgctgc tcggagacca gcggcccgat
       61 ctgcgtgttc gggtcgaggc cgttgccgac ctgcagcttc ttgccgtatt cggcgacgcg
      121 gccgacgaat tcgtcgtaca cttgatgctc gacgaacagc cgcgtgccgg cgctgcagat
      181 ctggcccgaa ttggcgaaca ccgccatcgc cgcacccggc accgccgcat cgagatcggc
      241 gtcggcgaac acgatgtccg gcgatttgcc gccgagttcg agcgacaccc gcttgagatt
      301 gcccgcggac gccttgacga tcgattgtcc cgtgacatgc gagccggtga aagcgacctt
      361 gtcgacgccg ggatgcgaag caagcgcagc acccgcggtc tcgccatatc ccggcaccac
      421 attgacgacg ccgggcggaa tgccggcttc catcgccagt tccgccagcc gcagcgacgt
      481 cagcggcgct tcttccgcgg gtttcaggat cacggtgcag ccggtggcga tggccggacc
      541 gatcttccag acgctggcgc cgagcgggcc attccatgga atgatcgcgc cgacgacacc
      601 aaccggctcc ttcagcgtgt aggaaaagat atcgccgggc agcgagttct cgatggtctc
      661 gccgtgcagc gcggtcgcct gccctgcata atatctgagc atgccgagca cgcgctgttt
      721 ggtgccgcgg gtgcggctga tcggcgcccc catatccagc gtatccagcg tcgagagttc
      781 atcaaaattc ttctcgacca gatcggcaag cttgagcagc agagcctgcc gctcatatgg
      841 cttgaccttg ctccacggtc cttcgaaggc gcggcgtgcg gctgcgaccg cgcggttgat
      901 atcctcggca tcgccctcgg caacggtcgc cagcaattcg ccggtcgccg ggttatgggt
      961 ctcgaagcgc ttgccggacg cggcgtcgat ccatttgccg tcgatcagca tctttttgta
     1021 ggaaccatcc gcataaggat gacgggtcat cggtattgcc tgcgtcacag ccatatctgc
     1081 actcccttga tatcgatcga tcgtcagaac gttatatcca gatatatata gcggacacgc
     1141 tgccccgtaa aagacgcgtt cgcggccgac gaaatggcgt cgaagaaaaa ccacccatgc
     1201 cgtttcgcgc atgggtgtta cgccatattt tcataccgaa tgacggaaat attatgtcga
     1261 tctgttcgat tgttcggcgg gacaatcctc accgcgtcag gagaactcac gtgccccacg
     1321 ccgccatgtc agccaataat gtcgccgttg ttaccggagg cgcgtccgga ttaggccttg
     1381 ctgccgccat ccggttcgct cgtttcggta tgaaagtgtg tatcgcggat ctggattcca
     1441 accgtcttgc ggaagcagcg accaaattgt cgtcggtggc gccgggtggc gcaaccgatg
     1501 tcatggtgac ggccgttgac gtcagccgca tcgaagacgt gtcggaactt gaaatcgccg
     1561 tacgcgagcg gttcggcggc accgatatcc tgatgaacaa tgccggcatc cagccgggca
     1621 gcgagatgtt cgggccgctg caaaactggc ggcgcattat cggcgtgaac ctctggggcg
     1681 tgattcacgg gtcgcaggtc tttgcaccca gcatgatcga gcgtgggcgc cccggcctca
     1741 tcatcaatac cggctcgaag cagggcatca cgacgccacc gggcgatccc gcgtataatg
     1801 tttccaaggc aggcgtcaaa gccttcaccg aggcgctgca acacgagttg cgaaacaccg
     1861 ccggcggccg gatcagcgct cacctgctga ttccgggttt tgtattcacc gggctgaccg
     1921 ccaagggccg caccgagaag ccggccgggg cctggacgcc ggcgcagacc atcgacttca
     1981 tgatcgagcg gctggacgcc gacgatttct acatcctgtg cccggataac gacgtgccgc
     2041 gacgcctgga cgagcggcgg atcctctggg cggcaggcga catcgtcgag aaccgccctg
     2101 ccctgtcgcg ctggcatccg gattatgcgg aggcgtttgc ggcgttcgtg aaggattctt
     2161 aagacccgtc attgcgagcc aagcgaagca atccaccgac gcggatgatg cagccagcgc
     2221 aaccattatt gccatcagcc atgcacgcgt taagtctttc caattttcgt cattccccga
     2281 tgtgcaattg cacatcgtgg atgcgccact tggcgcaggc ccggaatcca tactccctgt
     2341 cgtggttatg gattccgggc tcgcgcggag cctgtcatcg ggccgcgctt tgcgcggacc
     2401 cgttggcgcg ccccggaatg acgaggtgtg aaaactgaac ccgcgtcact ataaaccgca
     2461 aacgccacta gtgcgcccgc aacgtcgctt tcggcgcagg ctgcgcgacc agcgcctcgc
     2521 cgagttcgct ctgctgctgg cgcgggatgg agaagcagac gctaaatccg tccacggtcc
     2581 gcaacgtcac catgccttgc gtcggatcgg tcgattgctc gaccacccat gacgccagcg
     2641 ggtaggcata gcgcaggctt tgatcaccaa agcgtgtctg cagcgccttg tcgagcagcg
     2701 acggcagcgt catggcgagc gcgccgacct ggttcaggga cagccggatc gtcgctggcg
     2761 tgccggtgga atcgacaaaa cccagcgaaa tcgcgccacc atcggcggca acctcgcagg
     2821 tcgtgagggc ctgggtgtcg atctccatgg tcaagctccg gacaattatg cgttatattt
     2881 ttctgaatat atcgttcttg ccagcgacgt ccagcccggt cttgggccga tcgatgagcc
     2941 agcgggccgt agtccgccgg ccaaatccgt gatatacgtt aaaatataac gagacctcca
     3001 aagcggtccg ttcgaaaggg agagcaagcc aatgagcgat ttcaaccttc tcatcgacgg
     3061 gaaaatggtt cccggcgatc aaacgatgcc cgtcctcaat cccgcgaccg aggaagtgct
     3121 ggcgcaatgc ccgcgcgcct ccaagagcca gctcgacgcc gctgtcgccg cagccaaggc
     3181 cgcctttccg ggctgggccg cgaccccgat cggggagcgc cgcaagctgg tgacaaaaat
     3241 ggccgaggcc atcgaagcga atgtgggcga attggcgcgg ctgttgacct gcgaacaggc
     3301 aagccgcttg ccgacgccac cggcgaagtc atgggcatgg ctgccttctt ccgttatctc
     3361 agttcgctcg acctgccgac gcgggtaatc gaggacagcg gtgaccgccg cgtcgaggcg
     3421 catcgccgtc cgctcggcgt cgtcggcgcc atcatcccct ggaattatcc gctgctgatc
     3481 ctcagcttca aattgccgtc ggcgctattg gccggcaaca cgctggtcgt gaagccggcg
     3541 ccgaccacgc cgctgtccac cctgaaattc gccgaactgg tcaaggacat cctgcccaag
     3601 ggcgtgttga acgtcatcac cgatgccaac gatcttggcg acgccatgac gaagcatccg
     3661 gacattcgca agatctcgtt caccggttcg accgcgaccg gccagaaggt catggcgagc
     3721 gcggcgcaaa ccctgaagcg gatcacgctg gaactcggcg gcaacgacgc cggcatcgtg
     3781 ctcgacgatg tcgatccgaa gaaggtcgcg cccggcatct tcgaaggcgc gttccagaac
     3841 tcaggacagg tctgtctcgc catcaagcgg ctctatgtgc acgagtcggt ctatgacgaa
     3901 atctgcgacg aactggtggc gatcgccaaa agcacggtcg tggacgatgg ctcggggcac
     3961 caagctcgga ccgctgcaga acaagatgca gtacgagaag gtgaaggttt ttctggaaga
     4021 cgcccgcaag aacggcaata tcattgccgg tggtgctgcg atggatcgcc ccggttattt
     4081 tattcaaccg accatcgtgc gcgacatcaa ggaaggctcc cggctggtcg acgaggaaca
     4141 gttcgggccg gtgctgccgg tgatcaaata ttccgacaat gacgacgtga tccgccgcgc
     4201 caacgccacc aattacgggc tgggcgcgtc ggtctggtcg tcgaatacgg cacgggcgca
     4261 tcaggtcgcg agccagatcg aagccggcac ggtatggatc aacaagcacc tcgacatggc
     4321 gccgcatatt ccgttcggcg gggccaagca gtcgggcatc ggcaccgagt tcgccgagga
     4381 agggcttgcg gagttcaccc agctgcagat catcaacgag gcacgcacgt aacgtgacat
     4441 ggatgatcgc ggcggcgctg ccgccgcggt cacacttttt gaacgtcgca agatggagaa
     4501 gctgcgtcat gtttgactcg caatgcgacc tcgcggcgct ggtctacgag caggatcaga
     4561 atccggacga agtgctgcgc caatttgcgt ccgacctgaa cgcaagcggc tatcgcgcgg
     4621 tcggtctggt gcaactcggc catcattgcc tcgatgcacc gaaattgtcg gccatgctgg
     4681 tccataccgg cgaagagctg cagctgttcc agaacctcgg cgcttgcgcg acgggctgcc
     4741 ggctggatgt cgggcagttg ctggacgcag gctcccaagt cgccagcgcg atcgatcagg
     4801 gcgccgatct tgtgatcgtc aaccggttcg ggcggcagga gcgcgaagga aaaggccttt
     4861 catttctggt cgagcgcgct ttgagcgcgg acgtcccggt ggtgatcgca gtgccgagcc
     4921 accgcttcgc ggactggatc aggttcgccg acggcatgag cgtcaagctg cgctgcgacc
     4981 gcgcggccct ggatgcatgg tggcagaccg tctcggcccg ccacgccggg ctcgtccggc
     5041 aggaccatca gaccgtctgc gaagttttca agtaagccgg cggcgggcca aatttcaaag
     5101 cgtctcttgc ttgtggccgc attccttgca ggcgaatttc acccgcgtcg cgcccttggc
     5161 cgccgagacc cgatttggcg cgccgcattt tccgcaggtg gcctcgatgc gggtgttgcc
     5221 ctgcttcacc acaatggcct tgcgttccat ggtttcgcgg atcaggcgtt cggcctcttc
     5281 gcgcatcgac tgcttcgaca tccgctgtac ctgtcatgaa aaatcgccac gggagcagcc
     5341 ttataacacc cgcatcgcag ttttttgaca ccgatcgcga aaatcgccgt tgaagccgag
     5401 gatcggcgcg gcgctttcga ccaagttcac gatcccacga tttggcgccg cacggcagaa
     5461 atcgtgtatg acgcgcttat gtagtctcga caatcacata gctgtcctcg atgtgaaccg
     5521 ggaacgtttc ggccaaataa ggcccctttg ccagcgtctc gccgtcctcg atcacgaccg
     5581 gataggtgcg gatcttgacg cgggcgggat cgaagtagga ctgcccgttg cgcatgtcga
     5641 actcccagcc gtgccaggcg cagcgcaaca gttcgccgac ccgcgagcgc tgatagacac
     5701 cgggctccgg cgatgtcagc cgcgccacgc atgccgcctt gtcgagcggc gcaccctcat
     5761 gcggacagcg attgagcagc gcgaagaact cgccgttgac atggaacacg acgatgtcgc
     5821 ggccatcgac ggcaaccacc ttgttgccgc cgggcgggat gtctgtggtg cgggcgacga
     5881 tgtggcgggc catgttatca aaaacttgct gtgatcagag cttgtacaac gcgcgggcgt
     5941 tgccgttgaa gatttttgcg cgctcggctt ccgtcagcgg cgtcttgaac gcaaaccgcg
     6001 gatcgtcaaa gtcccaatgc ggataatccg acgagaacaa caggcgatcg acgccgatcc
     6061 actcgatcag ggatcgcaga tgttttgctt catccggttc gtcgatcggc tgtgtcgtga
     6121 accagaaatg ctgcgtcaca tattcggacg gacgccgctt caggtgcggg acctcgtcgc
     6181 ggaatcgctc aaaatgctgg tccatccgcc aggtggtcgc gggaatccag cccagcccgc
     6241 cttcgatgaa cacgattttc aggcgcggaa agcgctcggg tacgccttcg atgacgaggc
     6301 tggagagctg cgccgccacc gaatgcgcgt tcgattgatg ctcctcgacg taataggacg
     6361 gccagccgcc gccggtcggc gcgtggccgc catagccgcc gacgtggatg ccgagcggca
     6421 aatcaagttc ctgcgcgcgt gcatagatcg gccagtagcg gcggcggccg agcggttcgt
     6481 tggcgcgcgg acagacattg atctgcacat actggccaat tttcgcgcaa cgatcgatct
     6541 cggcgacggc tgcctcggta tcgtcctgcc ccacaagaat cgcggctttc aaccgaggct
     6601 cccggctggt ccagtgcgcg agctgccagt cgttgacggc gcgctggatc gccgcgccga
     6661 attcgaggtt ctgttgcgag aagatgaaca tatcgagcac ctgcagaatg ccgaactcga
     6721 tatcgagcgg atcgaggtgc tgcttgcgca tgaaggcgag atcggatccg ggcgggccgc
     6781 cggtcggcgg ccaggcatcg cggcgcgcga tcagcggcga ggaccgcgga tagggcgtgg
     6841 tgccgatata gggcgtgcgc agatgatcgc caaaggtctt catatgctgc tgccagcgct
     6901 ttgacaggaa cggattgagg tcggagcgcg catgcaggct cggatgcaca tcgcagtcga
     6961 tgatgcgcag ccggtccttc gaaggggctt ccgcttcaag cagcgggcgg tcgatgacgt
     7021 cgctcatgcg atcctccttc ggatcaaggt ttatgcggcg agagaaagcc gcggaaaggt
     7081 ttcaagcgga ttgtcggcgc acatccgcga gaccaggctt gccggcagat gcggcggtat
     7141 cgcgtcgtcg ccgtcgaatt gccagtgcgg ataatccgac gcgaacaaga acatcttgtc
     7201 cgagccgatc atgtcgatga tgtcggcgac gccggccgca tcgggcggcg cgtcgaacgg
     7261 ctgcgtggtc aggcggatgt ggtcgcgcat gatcgccgcc ggctcgcg
//
LOCUS       DASWGM010000117         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2503811,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000117 DASWGM010000000
VERSION     DASWGM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503811"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VGA15_03560"
     CDS             complement(<1..1049)
                     /locus_tag="VGA15_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586802.1"
                     /translation="MLKVTGAVLTAGLTFAAQAFAADAPAEIKIGTLYASSGRYASIS
                     MPVHSALKLWIDQKNADGGVYVKAFDKKIPVKLVAYDDQSNTATASTLYNQLITQDKV
                     DLLVADSGSVLTAPAVAIARDHKVFLFDQTGTGASFFSKDNPYIALMADPVSTIWPKP
                     VADFVSHDGPGLGIKKIAILYSTNEFTGTQANAFRKFVKDSGAPIEIVYDQGIPTETT
                     NYTVIINNINNTQPDAVIHFGYAPNDIAFLRNVQDVGVKFKMLFSIYAGLETELLEKN
                     VGEKGLEHVFTYVPPSELEYPVNFGMTMKDYKAAWDKKYPDGKIEFGFNAVAGYTTGL
                     VIEKTLSVATSLDQL"
     gene            1258..1668
                     /locus_tag="VGA15_03565"
     CDS             1258..1668
                     /locus_tag="VGA15_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEX9586803.1"
                     /translation="MAEPARARPKPTPETQHFWDGTQAGELRLQRCDACANVYFPPRP
                     FCPACASRKVSVFKASGKAKLYSYVIHHRPVPGFTPPYAIAVVELDEGPRMMSNILDC
                     PQTPEALELDMKLEVAFEKLDDTVTLPLFRPAKG"
     gene            1674..>2357
                     /locus_tag="VGA15_03570"
     CDS             1674..>2357
                     /locus_tag="VGA15_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HEX9586804.1"
                     /translation="MRRNQVAVVGAAETTELGVIPNVSQIQLHADAALNAIADAGLKL
                     SDIDGFATAVETPQQIAHYLGITPTWVDGTSVGGCSFMLHVRHAAAAIEAGLCKTVLI
                     THAESGKSNIGRTPRMIGPDSLNGQFELPYGVSTPASMFPIPVLRYMKTHGITHEQIA
                     MVAVVQREWAAKNPRATMKAPITVEDVLNSRMIAYPFRILQCCLVTDGGGALILTSAE
                     RARDFKTKPV"
BASE COUNT          470 a          726 c          704 g          457 t
ORIGIN      
        1 tcgagctggt cgaggctggt cgcaaccgag agtgtcttct cgatgacgag accggtggtg
       61 taaccggcca ccgcattgaa gccgaactcg atcttgccat cgggatattt cttgtcccag
      121 gcggctttgt aatccttcat ggtcatgccg aaattgaccg gatattccag ttccgatggc
      181 ggcacatagg tgaagacgtg ctcgaggccc ttctcgccga cattcttttc aagaagttcg
      241 gtctcgagac cggcatagat cgaaaacagc atcttgaact tgacgccgac gtcctggacg
      301 ttgcgcaaaa acgcaatatc gttcggcgcg tatccgaaat gaatcacggc gtccggctgg
      361 gtattgttga tgttgttgat gatgaccgtg tagttggtgg tttcggtggg aatgccctga
      421 tcgtagacga tctcgatcgg cgcgccggaa tccttgacga atttgcggaa ggcgttggcc
      481 tgcgttcccg tgaattcgtt agtcgagtaa agaatggcga ttttcttgat cccaaggccc
      541 ggtccatcgt gcgacacaaa gtccgcaacg ggctttggcc agatggtcga gaccgggtcg
      601 gccatcagcg cgatgtaggg attgtccttg gagaaaaagc tggcgccggt tccggtctgg
      661 tcgaacagga acaccttgtg gtcgcgcgcg atcgccaccg caggcgccgt caagaccgag
      721 ccggagtcgg cgaccaggag atcgaccttg tcctgcgtga tcagctggtt gtagagcgtg
      781 gaggcggttg cggtgttgct ctgatcgtca taggcgacaa gcttcacggg aattttcttg
      841 tcgaacgcct tcacatagac cccgccgtcg gcgttcttct ggtcgatcca gagtttcagt
      901 gcgctgtgaa ccggcatcga aatcgacgcg tagcgcccgg atgacgcata gagcgtgccg
      961 atcttgattt ctgccggtgc gtcggcggca aaagcctgtg cggcgaatgt caaacccgcc
     1021 gtcaataccg cgcctgtcac cttcaacatg aaatcctccc gattttcttc gctctgacgg
     1081 acaatctacc gcgcggggtc ggaggtacac tagcgctccg ggccaagcaa tagtgcgcgt
     1141 gccggaatac gcggcatgcg gcaggccagc tggaccgttg acgcaggacc gtccaatgtc
     1201 gcatcaagat cagagccggc gggcggacgt ggaccggccc agggaggaat cacaggcatg
     1261 gccgagccag cgcgcgcacg accgaaacca acaccggaaa cccagcattt ctgggatggc
     1321 acacaggcgg gcgagctgcg cttgcagcgc tgcgacgcct gcgccaacgt ctatttcccg
     1381 ccgcgcccct tctgcccggc ctgtgcctcg cgcaaggtca gcgtgttcaa ggccagcggc
     1441 aaggcaaaac tctacagcta cgtcatccat caccggccgg tacccggctt cacgccgcct
     1501 tacgccatcg ccgtggtcga gctcgacgaa ggtccgcgca tgatgagcaa catcctcgat
     1561 tgcccgcaaa cgccggaggc gctcgaactc gacatgaagc tggaagtcgc gttcgaaaag
     1621 ctcgacgaca ccgtcaccct tcccctgttc cgtccggcga agggctaagc accatgcgca
     1681 gaaatcaggt cgccgtcgtt ggcgctgccg aaaccaccga gctcggcgtt attccaaatg
     1741 tgtcgcagat ccagttgcat gccgacgcag ccctcaacgc catcgccgac gccggcctaa
     1801 aactgtccga tatcgacggt tttgctaccg cggtcgaaac accgcagcag atcgcgcatt
     1861 atctcgggat cacaccgacc tgggtcgacg gtacgtcggt cggcggctgc tcgttcatgc
     1921 tgcacgtccg ccacgccgcg gcggcgatcg aggctggcct ctgcaagacc gtgctgatca
     1981 cccatgccga aagcggcaaa tcgaatatcg gccgcacgcc gcgcatgatc ggccccgaca
     2041 gtctcaacgg ccagttcgaa ctgccttacg gtgtctcgac gccggccagc atgtttccca
     2101 taccggtgct gcgctacatg aagacccacg gcattaccca tgagcagatc gccatggtcg
     2161 cggtggtgca gcgcgaatgg gccgcgaaga acccgcgcgc caccatgaag gcgccaatca
     2221 cggtcgagga cgtgttgaat tcgcggatga tcgcctatcc gttccgcatc ctgcaatgct
     2281 gcctcgtcac cgacggcggc ggcgcgctga tcctgacctc ggccgagcgc gccagggatt
     2341 tcaagaccaa gccggtc
//
LOCUS       DASWGM010000118         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_285530,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000118 DASWGM010000000
VERSION     DASWGM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285530"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..1520
                     /locus_tag="VGA15_03575"
     CDS             369..1520
                     /locus_tag="VGA15_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX9586805.1"
                     /translation="MLRGIALFGVLAINVVNEFRVSIFEEFLPVADTTGTLDRAVQTF
                     LTMAIELKAMALFSLLFGIGMAIQFERLAPNPRRGMLLVRRLAILLVIGVAHLFLIWN
                     GDILTEYSLAGFIALPFLWGPRWLLAAGASVFLALYLVMPLLPPVVPWPTTAWITNHV
                     AEARQVYGTGGFLEILTFRIGETASILPLHVFILPRTIALFLFGMFVWRTDVVRRPPT
                     HGRLLVTVAIVGVLVGAALTIASEEPALLGLPLPGRMGASLERLATLLLATGYAAAII
                     GFVSTRPGKRLLAWAAPLGRMAFTNYLAQSVVFGWIFYGYGLGLFGRVGVVTALAIGV
                     CVYVAQVMISAQWLRYYRFGPIEWLWRSLMYGTAQRLRIRQAIPSGTSA"
     gene            1616..1834
                     /locus_tag="VGA15_03580"
     CDS             1616..1834
                     /locus_tag="VGA15_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586806.1"
                     /translation="MASSRKMPRISRFAFAPAGIALFSAGQALASQGPGGGPGTASGF
                     TQLAMAIIVYGTSALVVCTGLIGAARGR"
     gene            complement(1972..2214)
                     /locus_tag="VGA15_03585"
     CDS             complement(1972..2214)
                     /locus_tag="VGA15_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586807.1"
                     /translation="MRTAQASQPMVTEASAQRRVRRPPTRLRVYPSYQGGFDDVYPRY
                     FPGRNAVRDCNATYVQEYRPSGTVIVPHVNCYWRPG"
     gene            2438..2836
                     /locus_tag="VGA15_03590"
     CDS             2438..2836
                     /locus_tag="VGA15_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX9586808.1"
                     /translation="MWTKAKAPSLAEMEAMAHQIFEHLPKGFRKLCEGVIIRVDDFPT
                     DEVLDEMDAANEFDLLGLFQGIGLPFRSHDDIARLPNMVWLYRRPILDYWAEHDETLG
                     HIVRHVLIHEIGHHFGLSDSDMEAIEAAAG"
     gene            2930..>3500
                     /gene="leuD"
                     /locus_tag="VGA15_03595"
     CDS             2930..>3500
                     /gene="leuD"
                     /locus_tag="VGA15_03595"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9586809.1"
                     /translation="MEKFTTLEGVAAPLKIINVDTDMVIPKQYLKTIKRTGLGKGLFA
                     EQRYKDDGSENPDFILNKPAYRNAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTS
                     FGDIFYNNCFKNGILPIRVTQDDLDKLFDDAERGANATLSVNLAKQEIRGPDGGTVKF
                     EIDPFRKHCLLNGLDDIGLTMEKKASIDAY"
BASE COUNT          660 a         1097 c         1018 g          725 t
ORIGIN      
        1 gcggcgacgg ccgcaagccc cagaactctc atcacgttca tatgtttcct ccgttggtca
       61 ttggacaaca cgccagcgac gtgcggcgga tctggtcgac cgcctcaccg caacgcagat
      121 cgtaaagcta gagtgactga cgtgaatgtg gaatgaatag ctgcgccgcg tagatgaatg
      181 cgaatggtgc gaaccggttc ccgccgggca taattatctt gtcgacgtgt tcaaaatagc
      241 tcagttgagc ggctccaagt cgagcaactc ttttcagagg accctccatg gtcgagatcg
      301 ttgcggttgg cgctgcgccg gtggcctcgg ctcccgtcaa tcccagcgaa cgcatcgatg
      361 ccatcgatgt gcttcgaggc atcgccctgt tcggcgtgct cgcgatcaac gtcgtgaacg
      421 agtttcgcgt ttccatcttt gaagagttct tgcccgtcgc agacaccacg ggaacgcttg
      481 accgcgccgt ccaaaccttc ctgacgatgg ccattgagtt gaaggccatg gccctgttct
      541 cgctgctgtt cgggatcggc atggccatcc agttcgagcg gctggcgcca aatccgcgcc
      601 gcggcatgct gctggtgcgc aggctggcga ttctgctggt catcggcgtg gcgcatctgt
      661 ttctgatatg gaacggcgac atcctcaccg aatacagtct ggcgggattc atcgcgctgc
      721 cattcctgtg ggggccgcgt tggctgctgg cggccggcgc gtcggtattt ctggcgcttt
      781 atctggtcat gccgctgttg cctccggtcg tgccgtggcc caccacggct tggatcacga
      841 atcatgtcgc cgaagcgcga caggtctatg gcacgggcgg atttctggag atcctcacct
      901 tccgtatcgg ggagacggca tccatccttc cactccatgt cttcattctt ccgcgcacca
      961 tcgcgctatt cctgttcggc atgttcgtgt ggcgcacgga cgtcgttcgt cgtccaccga
     1021 cccacgggcg tctgctcgtc accgtcgcga tcgtcggcgt gctcgtcgga gccgcgctta
     1081 ccatcgccag cgaagagcct gccctgctgg ggctgccgtt gcccgggcga atgggcgctt
     1141 ccctcgaacg gcttgccacg cttctgctgg cgacaggcta tgcagccgcc atcattggtt
     1201 tcgtgagcac ccgaccggga aagaggctgc tggcatgggc cgcgccgctg ggacgcatgg
     1261 cttttacgaa ttacctggcg caatccgtcg tcttcggttg gattttctac ggctatggtc
     1321 tcggcctgtt cggccgcgtg ggggtcgtca ccgcactcgc gatcggtgtc tgcgtgtatg
     1381 tcgcccaagt gatgatcagc gcccaatggc tgcggtacta tcggttcggc cccatcgagt
     1441 ggctgtggcg cagcttgatg tacggcacag cgcagcgctt gcgcatccgc caagccatcc
     1501 cttcaggcac gtccgcgtag ctgacgacag gggcggtttg gcacgacaac atttctaaaa
     1561 ccggcataga ctttcgtccg tatcttctga acgcgtccca tcacaggaga ttgtcatggc
     1621 atcgtctcga aaaatgcccc ggatatcgcg cttcgctttc gcacctgctg gtatcgcctt
     1681 gttttcagcg ggccaagccc tcgcatccca gggacccggc ggaggtccgg gcaccgccag
     1741 cggcttcacg caactcgcca tggcgattat cgtctatgga acctcggcgc tggtggtctg
     1801 caccggtctg atcggtgcgg cgcgcgggcg ttgagagcgg caatatcgca acgcgtttca
     1861 agcgaaagcc tgccccggac ctgacccggg gtggttgccg gttcgcgtca agaaaacgcg
     1921 tcaaaagaaa gacagcgcct cgattctgat gcaatcagga ccgatcgggt tctagcccgg
     1981 ccgccaatag caattgacgt gaggcacaat caccgtgccg ctcggccggt attcctgcac
     2041 gtaagttgcg ttgcaatctc gcaccgcatt gcggccggga aaataccggg ggtagacgtc
     2101 gtcgaatccg ccctggtagc tcggatacac acgcaagcgt gtcggcggcc gccggacccg
     2161 gcgctgcgcc gacgcctcgg tgaccatcgg ctgcgatgcc tgcgccgtcc gcattcccgc
     2221 cggcaccgtt tgggcctcga cgccggctgt gggcagcgaa gaaacgcctg caaaaaccag
     2281 caatattgcc gccgtccgga tccgcatcat tccgcccacc caaagctgtc taacggccgc
     2341 aacgtctccg attgccgcgc caacgtcaac tgcacgtgaa gtccaatatt caaaccggga
     2401 tgcacttctg cgtaacacgc ggtaaaaagc cgtgatgatg tggaccaaag ccaaagcgcc
     2461 ctcgcttgcc gaaatggaag ccatggcgca ccagatcttc gagcacctgc cgaagggctt
     2521 tcgcaagctt tgcgagggcg tgatcatccg cgtcgacgat tttccgaccg acgaggttct
     2581 ggatgaaatg gacgcggcaa acgaattcga tctgctcggc ctgtttcagg gcataggcct
     2641 gccgttccgc agccatgacg acatcgcccg cctgcccaat atggtctggc tctaccgccg
     2701 gccgatcctg gattattggg ccgaacatga cgagacgctc ggtcatatcg tccgccatgt
     2761 gctgattcac gagatcggcc accattttgg tctgtctgac agcgacatgg aagcaatcga
     2821 ggcggcagca ggttaggatt gcgcaattcg gcctttgatt gagcccctat tcgcgataaa
     2881 ccaccgccca acggcctatc cttcaatttt tgccgggaag tgcgttcaaa tggaaaaatt
     2941 caccacgctg gaaggcgtcg cggcgcccct gaagatcatc aatgtcgata ccgacatggt
     3001 gattcccaag caatatctga aaaccatcaa gcgcaccggc ctcggcaagg ggctgttcgc
     3061 cgaacagcgc tacaaggatg acggcagcga gaacccggat ttcatcctca acaagccggc
     3121 ctatcgcaac gccaaaatcc tggtcgcggg cgacaatttc ggttgcggtt cgagtcgcga
     3181 acacgcgccc tgggcgctgc tcgatttcgg catccgctgc gtgatctcga cctcgttcgg
     3241 cgacatcttc tacaacaatt gtttcaagaa cggcattctg ccgatccgcg tcacccagga
     3301 cgacctcgac aaactgttcg acgatgccga gcgcggcgcc aatgcgacgc tgtccgtcaa
     3361 tctcgccaag caggagatcc gcggccccga cggcggcacg gtgaagttcg aaatcgatcc
     3421 attccgcaag cactgcctgt tgaacggcct cgacgacatc ggcctcacca tggagaagaa
     3481 ggcctcgatc gacgcctatg
//
LOCUS       DASWGM010000119         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2503860,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000119 DASWGM010000000
VERSION     DASWGM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503860"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGA15_03600"
     CDS             complement(<1..384)
                     /locus_tag="VGA15_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9586810.1"
                     /translation="MAADGNGKAANGKGGPATNGHAYPIEDHTYDVVVVGAGGAGLRA
                     VVGCSEAGLRTACITKVFPTRSHTVAAQGGISAALGNMHEDNWRWHMYDTVKGSDWLG
                     DQDSIEYMVRHAPEAVYELEHWGVPF"
     gene            complement(388..795)
                     /gene="sdhD"
                     /locus_tag="VGA15_03605"
     CDS             complement(388..795)
                     /gene="sdhD"
                     /locus_tag="VGA15_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX9586811.1"
                     /translation="MTAPNSPVAKSMRTPLARVRNLGASHSGTSDFWRQRVTAVAMVL
                     LIVPVIVVVMMLLGRNQAGAAQILGSLPIAIIMLLFIIASAWHMKIGMQVVIEDYVQS
                     ETLKLAAVMANNFFSIVVALASIYAILKLSSGV"
     gene            complement(792..1190)
                     /gene="sdhC"
                     /locus_tag="VGA15_03610"
     CDS             complement(792..1190)
                     /gene="sdhC"
                     /locus_tag="VGA15_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX9586812.1"
                     /translation="MTARIERPLSPHIQTYRWTLTMALSIVHRITGIALYFGTLLLAW
                     WLIAAASGPGAYANVQAFTGNFIGRLIVFGYTWALLHHLLSGLRHFVWDLGYGFKASE
                     REALTWGALIGGISLTVLLWIAAYAIGGGR"
     gene            complement(1534..>2067)
                     /locus_tag="VGA15_03615"
     CDS             complement(1534..>2067)
                     /locus_tag="VGA15_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912743.1"
                     /note="catalyzes the formation of malonyl-CoA from
                     malonate and CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HEX9586813.1"
                     /translation="TVRVTDPENGKELARETIGMIEVKGPNVFKGYWRMPEKTKAEFR
                     DDGFFITGDLGKIDGKGYVHILGRGKDLVISGGFNVYPKEIESEIDAMPGVIESAVIG
                     VPHADFGEGVTAILVCDKDAKVDETSVLKALDGRLAKFKMPKRVIVVEELPRNAMGKV
                     QKNILRDTYSEIYAKAK"
BASE COUNT          391 a          639 c          644 g          393 t
ORIGIN      
        1 aaacggcacg ccccaatgct cgagttcgta gaccgcctcc ggcgcgtggc gcaccatata
       61 ttcgatcgaa tcctgatcgc cgagccagtc cgaccccttg acggtgtcgt acatatgcca
      121 gcgccaattg tcctcgtgca tattgccgag cgccgccgag atgccgcctt gtgctgcgac
      181 agtatgcgag cgcgtcggaa ataccttggt gatgcaggcg gtgcgcaagc cggcttcgct
      241 gcagccgacc acggcgcgca ggccggcacc gccggcgccg accacgacga catcataggt
      301 gtgatcttcg atcggatagg catgaccgtt ggtggcggga ccacccttgc cgtttgcggc
      361 cttgccgttg ccatcagccg ccatgcgcta cactccggat gacagtttga ggatggcgta
      421 gatcgaggcc agcgccacca caatcgagaa gaaattgttg gccatcacgg cggcgagttt
      481 cagcgtctcg ctctgcacat agtcctcgat caccacctgc atgccgatct tcatgtgcca
      541 ggcgctggcg atgatgaaca gcagcatgat gatcgcgatc ggcagcgagc cgagaatctg
      601 cgcggcaccg gcctggttgc ggccgagcag catcatcacc acgacgatca caggcacgat
      661 cagcagcacc atcgccaccg cggtgacgcg ctggcgccag aaatccgacg tgccggaatg
      721 cgaggcgccg aggttgcgga cgcgggccag cggggtgcgc atcgatttgg cgaccggaga
      781 gtttggcgcg gtcatcgtcc gcctccgatc gcgtaagccg cgatccacag cagcaccgtc
      841 agcgaaatcc cgccgatcag cgcgccccag gtgagcgctt cacgctcgct tgccttgaag
      901 ccgtagccga ggtcccagac gaaatgcctg agaccgctga gcaaatggtg cagcagcgcc
      961 cacgtatagc cgaatacgat caggcggccg atgaagttgc cggtaaaggc ctgcacattc
     1021 gcgtaagcgc cggggccgga ggccgcggcg atcagccacc acgccagcag caaagtgccg
     1081 aaatacagcg cgatcccggt gatacgatgg acgatggaca gcgccatcgt cagggtccag
     1141 cggtaggtct gtatgtgagg cgaaagcggg cgttcgatcc tggcggtcat cggcggcttt
     1201 gacggtttgc gggcccgcat tggggccctt cggggaacgc ggttggcgcg tcctatttac
     1261 gaagtcgaaa cagtcagcgc aacggcgaaa tccgccgttt gcgaatgaat gttcatagct
     1321 tcagatgagc cctgcattga tggggattga cagaatttcc cggcactatc ggccagcgcg
     1381 gatgaagccc gattcatacc gcgcatgcgt ggtcaacgcg cgggcggaaa agggcggcaa
     1441 atggtggcag ggtatggcgg tcctcaggcc atggtgactg ccggtttggg ctcagtctgc
     1501 tgtgatgaag cggcgcgctc ccgccgccgt acttcacttc gccttggcgt agatctccga
     1561 ataagtatcg cgcaagatat tcttctgcac cttgcccatg gcgttgcgcg gcaactcctc
     1621 gaccacgatt acccgtttcg gcatcttgaa tttggcgagc cggccgtcga gcgccttcag
     1681 caccgaggtc tcatcgacct tggcgtcctt gtcgcacacc aggatagcgg tgacgccctc
     1741 gccgaaatcc gcgtgcggca cgccgatcac ggcggattcg atcacgcccg gcatggcgtc
     1801 gatctcgctc tcgatttcct tcgggtagac gttgaagccg ccggagatca cgagatcctt
     1861 gccgcggccg agaatatgca catagccttt gccgtcgatc ttgccgagat cgccggtgat
     1921 gaagaaaccg tcgtcgcgga attcggcttt tgtcttctcg ggcatccgcc agtagccctt
     1981 gaagacgttc gggcccttca cttcgatcat gccgatggtt tcgcgcgcga gttccttgcc
     2041 gttttcggga tcggtgacgc gcacggt
//
LOCUS       DASWGM010000120         4210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1254926,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000120 DASWGM010000000
VERSION     DASWGM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254926"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VGA15_03620"
     CDS             complement(<1..560)
                     /locus_tag="VGA15_03620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX9586814.1"
                     /translation="MGETVTIQKNWQELIRPNKLQVTPGSDSNRFATLVAEPLERGFG
                     QTLGNALRRILLSSLQGAAVQSVHIDGVLHEFSSIAGVREDVTDIVLNIKDISIKMQG
                     EGPKRMVVKKQGPGVVSAGDIQTVGDVVVLNPDLQICTLDEGAEIRMEFTVAAGKGYV
                     AAERNRPEDAPIGLIPVDSLFSPVRKV"
     gene            complement(679..1068)
                     /gene="rpsK"
                     /locus_tag="VGA15_03625"
     CDS             complement(679..1068)
                     /gene="rpsK"
                     /locus_tag="VGA15_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX9586815.1"
                     /translation="MGKEATRVRRRERKNIASGVAHVNSSFNNTTITITDAQGNTIAW
                     SSAGTMGFKGSRKSTPYAAQVAAEDVSKKAQEHGMRTLEVEVAGPGSGRESALRALQA
                     AGFTVTSIRDVTTIPHNGCRPRKRRRV"
     gene            complement(1202..1570)
                     /gene="rpsM"
                     /locus_tag="VGA15_03630"
     CDS             complement(1202..1570)
                     /gene="rpsM"
                     /locus_tag="VGA15_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX9586816.1"
                     /translation="MARIAGVNIPTNKRVLIALQYIHGIGQKNAAEILEKVKIPDDRR
                     VSQLSDQEVLQIREVIDRDYMVEGDLRRETGMNIKRLMDLGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPAKSIAGKKK"
     gene            complement(1752..2600)
                     /locus_tag="VGA15_03635"
     CDS             complement(1752..2600)
                     /locus_tag="VGA15_03635"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX9586817.1"
                     /translation="MRLILLGPPGSGKGTQAQRLVQRYGIIQLSTGEMLRAAVAAQTP
                     VGLKAKDIMASGALVPDEIVVGIISDRLDQPDAAKGFILDGFPRTVPQAEALDELLKR
                     KHMRLDAVIELRVNESALLQRVETRVAEMRARGEEVRIDDTQEVLTKRLASYRQLTEP
                     LIHYYSERRKLLTVDGMMTIEHVTREINRILTAIGAVETKGAKAAAAKRTAKAAKPAA
                     KTSKSAAKGAGAPKKTAKAYPKAGKPAAKGMKKSSRRLPASSKARAAGKPRKAAKKVT
                     KKRAKA"
     gene            complement(2597..3805)
                     /gene="secY"
                     /locus_tag="VGA15_03640"
     CDS             complement(2597..3805)
                     /gene="secY"
                     /locus_tag="VGA15_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9586818.1"
                     /translation="MKSELARRIVFTLGALLVFRLGSYIPLPGINAPGPQIPGVTVIS
                     GNRFSIFSLGILPYLSAAILIQLVSMVSSKLSALPSGGEAGRRKIARYTIGLAAILTA
                     FQGFGIASGLQGIPNLVNEPGGFFVISTTVTLTGGTIFLIWLSEQITARGIGNGLALI
                     LFAGVAAEMPHAVAVLVEYIRQGMLSAESVAVLAILWVALVGLVVFVELAHRRVPVEF
                     ASRKLGDRLLPAQSTHLSLKLNSAGMMPTVVAPWLYLLPLTVAGLVLDQTSPWLMAVY
                     GLVPQTHMARVILSSVAIIMLAFIYTAFVVDPEHAADSLNKHGGVIPGVEPGEPTAGH
                     LDRIVSYTTCIGALYLAAVFLIPELLLVYGEAPFYLGGTTVLVVVCTVLDIETQVRGR
                     SLTEPWGVFS"
     gene            complement(3802..>4210)
                     /locus_tag="VGA15_03645"
     CDS             complement(3802..>4210)
                     /locus_tag="VGA15_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX9586819.1"
                     /translation="QLGHGRPLFLILYLALIVFFAFFYTAIVFNPTETADNLKKHGGF
                     IPGIRPGERTAEYIDYVLSRITVLGAVYLAIVCLIPEILISYASVPFYFGGTSLLIVV
                     SVTMDTVAQVQGYLLAHQYEGLIRKSKLRGRRR"
BASE COUNT          832 a         1321 c         1252 g          805 t
ORIGIN      
        1 accttgcgca ccggcgagaa caggctgtcg accgggatca ggccgatcgg cgcatcctcg
       61 ggacggttgc gctcggcggc gacatagccc ttgccggcgg cgaccgtgaa ttccatgcgg
      121 atttccgcgc cctcgtcgag cgtgcaaatc tgcagatccg gattgagcac cacgacatcg
      181 ccgacggtct ggatatcgcc ggcgctgacg acgccagggc cctgcttctt cacgaccatg
      241 cgcttgggac cttcgccctg catcttgatc gagatgtcct tgatattcag cacgatgtcg
      301 gtgacgtcct cgcgaacgcc cgcgatcgag gagaattcgt gcagcacgcc gtcgatatgc
      361 accgactgca cggccgcacc ttgcagcgag gacaacagga tccggcgcaa cgcgttgccg
      421 agcgtctgac cgaagccgcg ctcgagcggc tcggcgacca acgtcgcaaa gcggttcgag
      481 tcgctgcccg gcgttacctg gagcttgttc ggccggataa gttcttgcca atttttctgg
      541 atcgtcactg tttcacccat gggccagtca attctgccaa ctaatcactg gcgttggaga
      601 ccgcggagaa ggcggcgtca catgtatttc aaagtcatca aagtcatcgc aggcggtcga
      661 accgcccgcg gccgaaaatc aaacccgccg acgcttgcgc ggacggcagc cattgtgcgg
      721 gatcgtcgtc acgtcgcgga tcgatgtgac agtgaaaccc gcggcctgca gcgcacggag
      781 cgccgattcg cggcccgaac cgggaccggc aacttcaacc tcgagcgtgc gcatgccgtg
      841 ttcctgcgcc ttcttggaga cgtcttccgc cgcgacttgc gcggcatagg gggtcgattt
      901 gcgcgacccc ttgaaaccca tcgttcccgc cgacgaccag gcaatggtgt tgccctgcgc
      961 gtcggtgatg gtgatggtcg tgttgttgaa cgacgaattc acatgcgcga cgccggacgc
     1021 aatgttcttg cgctcgcggc ggcgtacgcg ggtggcttcc ttgcccattc cagaaccttt
     1081 ctaacgatct caacgccgcc gtaatgccag cggctacacc tgaaaggcga gtagtgagtg
     1141 gcgaatagcg aatagtaagg cttaacccct actcgccatt ggccacttgc tattcgccaa
     1201 attacttctt cttgccggcg atggacttgg ccggaccctt gcgcgtgcgc gcattggtgt
     1261 gggtgcgctg accgcgcacc ggcaggccgc ggcgatggcg caggccgcga tagcagccga
     1321 gatccatcaa acgcttgatg ttcatgccgg tctcgcgacg cagatcacct tcgaccatat
     1381 agtcgcggtc gatcacttcg cggatctgca acacttcctg gtcgctcaac tgcgacacgc
     1441 ggcggtcatc gggaatcttg accttctcca aaatctcggc cgcgttcttc tggccgatgc
     1501 catggatgta ctggagcgcg atcagaacgc gcttgttggt cgggatattc acgccggcaa
     1561 tacgggccac ggacttctct cctgtcgcca atccctggtg gaacggctgt tttttgctat
     1621 ctcggcaggt gtttacaaac gcgaacacga cgcccttccc cttggttgtc ccggggcccg
     1681 gcatcgtctg aaaactatcc gactggatag gggacttatt aaaggattca acttggtttc
     1741 gtcaaccgtc gctatgcctt agctcgcttt ttcgtgacct ttttcgcggc tttcctgggt
     1801 tttccggcgg cccgggcctt ggaactcgca ggcagcctcc gagacgactt tttcatgccc
     1861 ttggcggccg gtttcccggc tttcgggtaa gccttggcgg tcttcttggg ggcaccggcg
     1921 cccttagccg cagatttgga ggttttggcc gccggtttgg cggcctttgc ggtccgcttc
     1981 gctgcggccg ccttggcccc cttggtctcc accgccccga tcgcagtcag gatacggtta
     2041 atctcccgcg tgacgtgctc gatggtcatc atgccatcga ccgtcaagag cttgcggcgt
     2101 tccgaatagt agtgaatcag cggctcagtc agctgacggt agctcgccag ccgttttgtc
     2161 agcacctcct gggtgtcgtc gatccgcacc tcctcgccac gcgcccgcat ctccgcgacc
     2221 cgggtttcga cgcgctgcaa cagcgcgctc tcattgacgc ggagttcgat cacggcatcg
     2281 agccgcatgt gctttctctt caggagctcg tcgagcgcct cggcctgcgg cacggtgcgc
     2341 ggaaagccgt cgaggataaa gcccttcgcc gcgtccggct gatccagccg atcggaaatg
     2401 atcccgacca cgatctcgtc cgggaccaga gcgccgctcg ccatgatgtc cttggccttg
     2461 aggccaaccg gcgtttgcgc tgcaaccgcg gcacgcagca tttcgccggt cgagagctgt
     2521 atgatgccgt aacgctgaac cagccgctgg gcctgggttc ccttgcccga ccccggcggc
     2581 ccgagaagaa tcagtctcat gaaaatacgc cccacggctc ggtgagcgac cggccgcgca
     2641 cctgtgtttc aatatcaaga acagtgcaaa ccacaaccag aaccgtcgtt ccacccaaat
     2701 aaaacggcgc ctcgccataa acgagcaaca gctcgggtat cagaaatacc gcggcgagat
     2761 aaagcgcgcc aatgcatgtc gtgtacgaca cgatacggtc gaggtgcccg gcggtcggct
     2821 cgcccggctc tactcccggg atcacgccgc catgcttgtt cagggaatcg gcggcatgct
     2881 cgggatcgac cacgaacgcg gtgtaaatga acgcaagcat gatgatcgca accgagctga
     2941 ggatcacacg ggccatgtgg gtctgcggga ccagtccgta cacggccatc agccatggcg
     3001 aggtctggtc caggaccaga ccggcaaccg tcaatggcaa caggtacagc catggagcga
     3061 cgaccgtcgg catcatgccc gcgctgttga gcttgagcga cagatgcgtc gactgtgccg
     3121 gaagcaaacg gtctccgagc tttcggctcg cgaattcaac cggcacccgc cgatgcgcaa
     3181 gttctacgaa aacgacgagg ccgaccaggg ctacccagag aatcgcgagc accgcgacac
     3241 tttcggcgga taacatccct tgccggatgt attcgaccaa cacggccacg gcatgcggca
     3301 tttccgccgc cacgcccgcg aaaaggatca gggcaaggcc gttgccgatg ccgcgcgcgg
     3361 tgatctgctc gctgagccag atcaggaaga tcgtcccgcc cgtcagcgtc accgtggtcg
     3421 agatcacaaa aaatccgccg ggctcgttga cgagattggg gatgccctga aggcccgaag
     3481 cgatgccgaa accctgaaac gccgtcaaaa tcgcggcaag accgatggtg taacgcgcga
     3541 tcttccggcg tccggcttcg ccgccgctgg gtagcgcact aagcttcgat gacaccatcg
     3601 agaccagctg aatcaggatc gccgcggaaa gatagggcag gatccccaac gaaaagatcg
     3661 aaaatcggtt gcccgagatg acggtgacgc cgggaatttg ggggcccggg gcgttgatgc
     3721 cgggaagcgg aatgtagctg ccgagtcgaa aaaccagcag cgcgccgagc gtgaaaacga
     3781 tccggcgcgc caactctgat ttcatcgacg ccggcctctc agcttcgact tcctgataag
     3841 accttcgtat tgatgggcga gcagataacc ctgcacctgc gccaccgtat ccatggtcac
     3901 gctgaccacg atcaacagcg acgttccacc aaaatagaac ggcaccgagg cgtaggaaat
     3961 caggatttcc ggtatcaggc agacgatcgc gagatagacc gcgcccagca ccgtgatgcg
     4021 cgacagcaca taatcgatgt attcggcggt gcgctcgccc ggccgaatgc ccgggatgaa
     4081 gccgccatgc tttttgagat tgtcggcggt ctcggtcgga ttgaacacga tcgcggtata
     4141 gaagaacgcg aagaacacga tcagcgccag atagagaatc aaaaacagcg ggcggccgtg
     4201 accgagctgg
//
LOCUS       DASWGM010000121         8383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_977734,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000121 DASWGM010000000
VERSION     DASWGM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977734"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..645
                     /locus_tag="VGA15_03650"
     CDS             4..645
                     /locus_tag="VGA15_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586820.1"
                     /translation="MTVAVAIAHAGQSFAQSAFPAPLPGRSAPVNDPAFPPVNGAAPS
                     ASVGAPPSSFPVTGAAPVTGSAFERGPASPSQGPSDECMKQFVPLREEAEKRGKLIKA
                     ASDRHASPDEACKLIGSFGQSEIKMIKYVETNASKCGIPAQIADQLKNGHKNTETMQK
                     KVCTVAQQGPQRGPAAPSLSEALGSSAALPEANLAAKKGGSTFDTLNGNVLAR"
     gene            656..1588
                     /gene="ubiA"
                     /locus_tag="VGA15_03655"
     CDS             656..1588
                     /gene="ubiA"
                     /locus_tag="VGA15_03655"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX9586821.1"
                     /translation="MSSTDARVADATGNWVDTHAPSWSRPYLRLSRLDRPIGSWLLLM
                     PCWWSAALAAGVIGDIGQLPLIIALFFIGAFAMRGAGCTWNDITDRDLDALVERTRSR
                     PIPAGQVSVPQAAAFLVVQALIGLAVLLQFNRFAVMTGIASLVIVAVYPFMKRITWWP
                     QIVLGLAFSWGALMGFAVTQQRIDATALALYAGSIAWVIGYDTIYAHQDAEDDALIGV
                     KSTALLFGARTRPALSVFYASAVVLIGVALALAGARWPAWIGLAAFAAHLIWQIKRLQ
                     INDPALCLRIFRSNRDAGLLLFAGVLVDAVMRAN"
     gene            complement(1744..2268)
                     /locus_tag="VGA15_03660"
     CDS             complement(1744..2268)
                     /locus_tag="VGA15_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6101 family protein"
                     /protein_id="HEX9586822.1"
                     /translation="MRRQTATSGINSAGSSRALRLDPHSLPISFNAHDTRADGGVRKI
                     ELHRERVVVRRALHGMRMAVNVRMRDFLGVALRGIDDAQMLVLVHRDPSLTIPLCVSS
                     DREEIASAWQMWSDIFALPQLPEDQAREPTMRRRRHNAIRARRPKFLVRRRRGSLLNP
                     ANIHQGEREISARD"
     gene            2583..3983
                     /locus_tag="VGA15_03665"
     CDS             2583..3983
                     /locus_tag="VGA15_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX9586823.1"
                     /translation="MISSPSAASSLSSKSASSDLFDQSALSALAQRLVEAAKRAGADA
                     ADAVAVRNVSQGVEVRDGRVEESERSEGDDVGLRVLVGRRQAVVSTNDISGDGVAKLA
                     ERAVAMARVAPDDKYVGLADPSLLARDFPDLDLLDPKVPSTPELERRAREAEAAALAV
                     KGVTKSGGASASTGIGGMVLVTSTGFHGSYLRSSQGISMTAISGDGTGMERDYDYTSA
                     LHASDLDSPAKVGRTAGERTVARANPRKVETCKVPVVFDPRVSGSLVGHLVGAVNGAS
                     IARKTSFLKDRLGEQLFAKNIRIIDDPLRVRGLRSQSFDAEGVKVKKLAIIDEGVLTS
                     WLLDSATARELDLVTTGHAHRGVSSSPSPGSYNLHLEAGEPTPAELISDIKQGFYVTD
                     LIGSGVNGVTGDYSRGASGFWIENGEITYAVSEVTIAGHLLEIFKSLAPANDLEFRYG
                     VNAPTLRIEGLTVGGR"
     gene            3973..4803
                     /locus_tag="VGA15_03670"
     CDS             3973..4803
                     /locus_tag="VGA15_03670"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX9586824.1"
                     /translation="MDASADSVHDRNPARDILTRDAGLLTDSVREAGALALSLFRTDL
                     KNWTKGASSPVSEADIRVNDLLESRLRSATPDYGWLSEESADDEQRLGKPLVWIVDPI
                     DGTRGYLAGREDWCVSVALVENGSTVLAAVFAPASDEFFFAARGQGAVRNAAPVFATS
                     GSELDFSRVAGPKPLVERLSRQSDEIILHPRIGSLALRLCRVAQGSLDAAFAGGQSRD
                     WDLAAANLIVQEANGNMTALSGDTIRYNRREVTHGVLVAAGRERHARIVEHFRERPLP
                     "
     gene            4863..5111
                     /locus_tag="VGA15_03675"
     CDS             4863..5111
                     /locus_tag="VGA15_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEX9586825.1"
                     /translation="MPDSAQPQLLHLVIGGELTDLEGTTFKDLDQVEIVGVFPNYASA
                     HAAWKAKAQQTVDNAHMRYFIVHLHRLLDPSQDTKPAR"
     gene            5108..5824
                     /locus_tag="VGA15_03680"
     CDS             5108..5824
                     /locus_tag="VGA15_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9586826.1"
                     /translation="MKKLLRNVLRSSWVQRAVGFLAAEYLRLVWLTNKFGFDPPDVYD
                     IVEPRMPAIFAFWHGQHFMTPFIRTKESHRAKVLISRHRDGEFNAIAAERLGVGTIRG
                     SGDHGSAFHRKGGVGAFKEMVRALEDNCNVALTADVPKRSRVAGLGIIMLARESGRPI
                     MPFAMATSRFIRLHNWDRTTINLPFGRGALVGIGAIVVPPDADAETMEKLRAELEANL
                     NEATRRAYAQVGRPEQAGHG"
     gene            5784..7121
                     /locus_tag="VGA15_03685"
     CDS             5784..7121
                     /locus_tag="VGA15_03685"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX9586827.1"
                     /translation="MRRSAVRNRRVMANSLPMTLRVYRKLSYAMVPLAPALIKRRLNQ
                     GKEDPLRIGERRGMSRDVRPHGPLVWIHGASVGEVLAAAALIERLRALNLRILLTSGT
                     VTSAAIVAKRFPADIIHQYVPYDSPRYVARFLDHWQPSLALFIESDLWPNLILSSAAR
                     RLPMVLINGRMSHRSFPRWRRISNTISALLGRFEVCLAQSQVDAERFAALGSRNVIAT
                     GNLKLDVPAPPADAAKLERLMSVTRGRPVIVAASTHPGEEEIWLETHRALAGSFPSLL
                     SVIVPRHPHRGEAIARTIAASGLHVALRSREELPTATTDIYVADTMGELGLFYRLAPI
                     VFMGGSLVSHGGQNPIEAVKLGASIVHGPHVFNFADVYEALDSAGGARRADTQEALVE
                     QLGALLADPAAREVSASAAERVVEQLGGALERTLAALEPYLLQLRLEIGPANA"
     gene            7114..8139
                     /gene="lpxK"
                     /locus_tag="VGA15_03690"
     CDS             7114..8139
                     /gene="lpxK"
                     /locus_tag="VGA15_03690"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX9586828.1"
                     /translation="MREPGFWHRPSSFASRLLMPLGALYGAVAARRLQREGFDAGVPV
                     LCVGNYHVGGAGKTPMVLALTKILRELGETPIVLSRGYGGQLRGPIKVDPERHAAVDV
                     GDEPLMLARTVPVVVARDRIGGVALARSQGASVILMDDGFQNPAIAKDASLIVIDGDR
                     GLGNGLVFPAGPLRAPLPPQLVRTDALIVVGDGAAAAPVAAAIAALGRPVLSAHFRAD
                     ASAASLNGKRVLAFAGIGDPVRFFRTLRASGIEIVRERAFADHHAYTQAEIEALIAEA
                     KRDALMLVTTEKDLARLRIGGQLAPYAQGIEPFAVTLEFDDAAQLRKFVSGRLFKARA
                     KKFRVNS"
     gene            complement(8239..>8383)
                     /locus_tag="VGA15_03695"
     CDS             complement(8239..>8383)
                     /locus_tag="VGA15_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEX9586829.1"
                     /translation="PGTYVRCAVTDARIPLDELKYWSVDLQEAYAIPTAVLQRHFPGA
                     LKA"
BASE COUNT         1416 a         2629 c         2758 g         1580 t
ORIGIN      
        1 ccgctgaccg tggccgtcgc tatcgcccac gccggtcaga gctttgcgca aagcgcattt
       61 ccggcgccgc tgccgggtcg atctgccccg gtcaacgacc cggcatttcc gccggtgaac
      121 ggcgcagctc catccgcgtc cgtcggcgca cctcccagtt catttccggt aaccggtgcg
      181 gcgcctgtca ccggctccgc gttcgaacgc ggaccggcgt ctccatcgca gggtccctcc
      241 gacgaatgca tgaagcagtt cgtgcccttg cgggaagagg cggagaagcg cggcaaactg
      301 atcaaggccg ccagcgaccg gcacgcgtcg ccggatgaag cctgcaagct gatcggaagt
      361 ttcggccagt ccgaaatcaa gatgatcaaa tatgtcgaga ccaatgcgtc gaagtgcggg
      421 atcccggcgc agatcgcgga ccagttgaaa aacggtcaca agaacaccga gacgatgcag
      481 aagaaagtct gcacggtggc gcagcaaggg ccgcaaagag gaccggccgc ccccagcctg
      541 agcgaggccc tgggttcttc ggcggcactg ccggaagcca acctcgctgc gaagaagggt
      601 ggcagcacgt tcgataccct gaacggcaac gttctcgcgc gatgaccgcg atcgaatgag
      661 cagcactgac gcccgcgttg ctgacgcgac cggcaactgg gtcgacactc acgcgccgtc
      721 gtggtcgcgg ccgtatttgc gcctttcccg cctcgaccgg ccgatcggat cctggctgct
      781 gctgatgccg tgctggtggt cggcggcgct cgccgccggc gtcatcggcg acatcgggca
      841 attgcccctc atcatcgcgc tgtttttcat cggcgcattt gccatgcgcg gcgccggctg
      901 tacctggaac gacatcaccg atcgcgatct cgatgcattg gtcgaacgga cgcgctcgcg
      961 gccgataccg gcaggacagg tgagcgtgcc gcaggcggcg gcctttctgg tcgttcaggc
     1021 cttgatcgga ttggcggtgc tgctgcaatt caaccgcttc gcggtgatga ccggcatcgc
     1081 ctcgctggtc atcgtcgcgg tctatccgtt catgaagcgg atcacctggt ggccgcagat
     1141 cgtgctcggg ctggcgttct cgtggggcgc gctgatggga tttgcggtga cgcagcagcg
     1201 gatcgatgcg acggcattgg cgctctatgc gggctcgatc gcctgggtga tcggctacga
     1261 caccatctat gcgcatcagg atgccgaaga cgacgcgctg atcggggtaa aatcgaccgc
     1321 gctgttgttc ggcgcgcgca cgcggcccgc gttgtcagtg ttttacgcat cggcggtggt
     1381 gttgatcggc gtggcgcttg cgttggccgg cgcgcgctgg ccggcctgga tcgggcttgc
     1441 cgcgtttgcg gcccatctga tttggcaaat caagcgtctg cagatcaacg atcccgcgct
     1501 atgcctgcgc attttcaggt ccaaccgcga cgcgggcctg ttgctgttcg cgggggtgct
     1561 ggtcgatgcg gtgatgcgcg cgaattaggc gctcgtcatc ctgaggcgct cgccggcttc
     1621 ggcgagcctc gaaggatgtg tacgcggccg tccttcgagg cgcgccaaaa gagcgcgcac
     1681 ctccagcgat aacggcgaag ccgttacgca gggatgacgt ccgcatatgc ggcgggaaag
     1741 aactcagtcc cgtgcgctga tctcgcgctc gccctgatga atattcgcag ggttgagcaa
     1801 actgccgcgg cgccgccgca ccaggaattt cggccgccgc gcccggatcg cgttgtggcg
     1861 ccggcgccgc atggtgggtt cacgagcctg gtcttccggc aattgcggaa gcgcgaagat
     1921 gtcgctccac atctgccaag cggacgcgat ctcttcacgg tcggagctga cgcacaaggg
     1981 aattgtcaat gacggatcgc gatgaaccag cacgagcatc tgggcgtcgt cgatgccgcg
     2041 cagtgccacg ccaaggaaat cgcgcatgcg aacgttgacc gccattcgca tgccgtggag
     2101 ggcacggcgc acgacgacgc gttcgcgatg aagttctatt ttccgcacgc cgccgtctgc
     2161 gcgggtgtcg tgcgcattga agctgatggg cagagaatgt gggtcgagcc gcaatgcacg
     2221 gctcgacccg gcggaattga tcccgcttgt tgcagtttga cgcctcacgg ctttatctcc
     2281 ccgccgggat tatgttcccg gtcgataggc gagaccgtag ccgagccgcg tcggcattgg
     2341 cttaaaaagg ctggttaatc ggaggtcact gggccggcat ccgcgcgcat gattgacaag
     2401 accttggccg acatgattga cgagaccttg ccgcgaaagc tgaaattctt cgctttgaac
     2461 gcaggaagtg cttgaagtct ttgtgaatca ggcacatctc atggtttgcg attgcattca
     2521 agcctgcttc gagtgtcctg cagtcctcct tcaattcctc ttcaatcctt gggattttgt
     2581 tcgtgatctc ttcaccatca gccgcatcgt cgctttcgtc gaaatccgcc agctccgatc
     2641 tgttcgatca atccgccctg agcgcgctgg cgcagcgtct cgttgaggcc gccaaacgcg
     2701 cgggcgcgga cgccgccgac gcggtcgcgg tgcgcaacgt ttcgcagggg gtagaggtcc
     2761 gggacggacg cgtcgaggaa tccgaacgct cggaaggcga tgatgtcgga ctgcgcgtgc
     2821 tggtgggacg gcgccaggcg gtggtctcga ccaacgacat cagcggcgat ggcgtcgcca
     2881 agctggccga gcgcgccgtc gccatggcgc gtgtcgcgcc cgacgataaa tatgtcggtc
     2941 ttgccgatcc gtcgctgctg gcgcgcgatt ttcccgatct cgatctgctc gaccccaagg
     3001 ttccctcgac gccggagctt gaacgccgcg cccgtgaagc cgaagcggca gcactcgcgg
     3061 tcaagggcgt gaccaaatcg ggtggcgcgt cggcatcgac cggaattggc ggcatggtgc
     3121 tggttacctc taccggcttt cacggctctt acctgcgctc gagccagggc atctcgatga
     3181 ccgcgatatc gggcgacggc accggcatgg aacgcgacta cgactacacc tcggcgctcc
     3241 acgcttccga tctcgattcg cccgccaagg tcggccgcac cgcgggcgag cgcacggtcg
     3301 cgcgcgccaa cccgcgcaag gtcgagacct gcaaggtgcc ggtggtgttc gaccccaggg
     3361 tttcgggatc gctggttggc catctggtgg gtgcggtcaa tggcgcatcg atcgcacgca
     3421 agaccagttt cctgaaagac cgtctcggcg agcagttgtt tgcgaagaat atccgcatca
     3481 tcgacgatcc gttgcgggtg cgcggattgc gctcgcaatc gttcgacgcc gagggtgtca
     3541 aggtcaagaa gctcgccatc atcgacgagg gggtgctgac ctcgtggctg ctggattcgg
     3601 cgaccgcgcg cgagctcgat ctggtcacga ccggccacgc ccatcgcggc gtatcgtcat
     3661 cgccgtcgcc gggatcgtac aatctccatc tcgaagccgg cgagcctacc ccggctgagc
     3721 tgatatccga catcaaacag ggattttacg tcaccgacct gatcggctcc ggcgtcaacg
     3781 gggtgaccgg cgactacagc cgcggcgcat ccggcttctg gatcgagaat ggcgagatca
     3841 cctatgcggt aagcgaggtg acgatcgcgg gccatctgct ggaaatattc aagtcgctgg
     3901 cgcccgccaa cgatctggaa tttcgttatg gcgtcaatgc tccgacgctg cgcatcgagg
     3961 gtttgacggt tggtggacgc tagcgccgac agcgtccatg acaggaaccc ggcgcgcgat
     4021 atcctgacgc gcgacgccgg gttgctgacc gattcggtgc gggaagccgg cgcgctggcg
     4081 ctgtcgctgt tccgcaccga cctgaaaaac tggaccaagg gcgcatcctc gccggtatcc
     4141 gaagccgata tcagggtcaa cgatctcctg gagagccggc ttcgctctgc gacgccggat
     4201 tatggctggc tgtcggagga aagcgccgac gacgagcagc gtctcggcaa gcctctggta
     4261 tggatcgtcg atccgatcga cggcacccgc ggttatctgg ccggccgcga ggactggtgc
     4321 gtcagcgtgg cgctggtcga gaacggatcg actgtactgg cggcggtgtt tgcacccgcg
     4381 agtgatgagt ttttctttgc cgcgcgcggg cagggcgccg tgcgcaacgc cgcgccggtt
     4441 ttcgcaacat ccggctccga gctggatttt tcccgcgtgg ccggtcctaa accgctggtt
     4501 gaacggctaa gccggcaatc ggatgaaatt atcctgcatc cccgtatagg atcgctggcg
     4561 cttcggctgt gccgggtcgc gcagggaagc ctcgatgcgg cgttcgccgg cggccagagc
     4621 cgcgactggg accttgccgc ggccaatttg atcgtgcagg aagcgaatgg taacatgacc
     4681 gcgctctcgg gggatacgat ccgctacaat cgtcgcgagg taacgcacgg ggtgctggtg
     4741 gcagcgggac gcgaacgtca tgcacgcatt gtcgagcatt ttcgagaacg tccgttgccc
     4801 tgagcgctgc cgatgcgcgt cgggaatcct gcttgccggg cagcgcgtta ggggaaaaaa
     4861 tcatgccaga tagcgcccag ccgcaattgc tccatctcgt catcggcggc gagttgaccg
     4921 atctcgaggg cacgacgttc aaggatctcg accaggtgga aatcgtcggt gtgtttccga
     4981 attacgcgag cgcccacgca gcctggaaag ccaaggcgca gcagaccgtc gacaacgccc
     5041 acatgcgcta cttcatcgtc cacctccacc ggctgctcga tccaagtcaa gacacaaagc
     5101 ctgcccgttg aaaaagctgc tccgtaatgt gctgcgcagc agctgggtcc agcgcgctgt
     5161 gggtttcctc gcggccgaat acctgcggct ggtctggctg accaacaaat tcggcttcga
     5221 tccgcccgac gtctacgaca tcgtcgagcc gcggatgccg gcgattttcg ccttctggca
     5281 cggccagcat ttcatgacgc cgttcatcag gaccaaggag agccatcgtg ccaaggtcct
     5341 gatctcgcgg catcgcgatg gcgaattcaa tgcgatcgcc gccgaacggc tcggggtcgg
     5401 caccatacgc ggctccggcg atcacggttc ggcgtttcat cgcaagggcg gcgtcggcgc
     5461 gttcaaggaa atggtgcggg cgcttgaaga taattgtaac gtcgcgctga ccgccgacgt
     5521 gccgaagcgc tcgcgggtcg cgggtctcgg cattatcatg ctggcgcgcg aatccggacg
     5581 gccgatcatg ccgttcgcaa tggcgaccag ccgcttcata cggctgcaca attgggaccg
     5641 caccaccatc aatctgccgt tcggtcgagg cgccctggtg ggcattggag cgattgtggt
     5701 gccaccggat gccgatgccg aaaccatgga aaaactgcgc gcagaactgg aagcaaacct
     5761 gaacgaagcg acccgtcgcg cctatgcgca ggtcggccgt ccggaacagg cgggtcatgg
     5821 ctaattcctt gccgatgacg ctgcgggtct accgcaagct gtcctacgcg atggtgccgc
     5881 tggcgccggc gctgatcaag cggcggctga accagggcaa ggaggatccg ctgcggatcg
     5941 gcgaacgccg cggcatgagc agggacgtcc ggccgcacgg accgctggtc tggattcatg
     6001 gcgccagcgt cggcgaagtg ctggccgcgg cggccctgat cgagagactg cgcgcgctca
     6061 atctccgcat cctgctgacg tcgggcacgg tgacctcggc cgcgatcgtc gccaagcggt
     6121 ttcccgccga tatcatccat caatatgtgc cttacgactc tccgcgctac gtcgcgcgct
     6181 ttctcgatca ctggcagccg agcctcgcat tgttcatcga gtccgatctg tggcccaatc
     6241 tcattctgtc gagcgcggcg cggcgtctgc cgatggtcct gatcaacggc cggatgtcgc
     6301 atcgctcgtt cccccgctgg cgcaggatca gcaacacgat ctcggcgctg cttggccggt
     6361 tcgaggtctg cctcgcccaa tctcaggtcg atgcggagcg ctttgcggcg ctcggcagcc
     6421 gcaacgtcat cgccaccgga aacctgaaac tggatgttcc ggcgccgccg gccgacgccg
     6481 ccaaattgga gcgcctgatg tcggtaacgc gcggccgtcc ggtcattgtc gcggcatcga
     6541 cccatcccgg cgaagaggaa atctggcttg aaacgcaccg ggcgctggcc gggtccttcc
     6601 catcgctgct gagcgtgatt gtgccgcggc atccccatcg cggtgaggcg atcgctcgca
     6661 ccatcgcggc ttccggcctg cacgtggcgt tgcgttcgcg cgaggagttg cccaccgcaa
     6721 ccaccgacat ctacgtcgcg gataccatgg gcgaattggg attgttttat cggctggcgc
     6781 cgatcgtgtt catgggaggc tcgctggttt ctcatggcgg gcaaaatccg atcgaagcgg
     6841 tcaagctggg cgcgtcgatc gttcacggcc ctcacgtctt caatttcgcc gacgtctacg
     6901 aggcgctcga tagcgccggc ggggccagac gggctgacac gcaggaggcg ctggtggagc
     6961 agctcggcgc gttgctggcc gatcctgcgg cgcgcgaagt gtcagccagt gccgccgaac
     7021 gcgtggtcga gcagctcggc ggggcgcttg agcgcaccct ggcggcactc gagccgtatc
     7081 tgctgcagtt gcggctcgag atcgggcccg ccaatgcgtg agccgggctt ctggcaccgg
     7141 ccatcgtcat ttgcatcgcg cttgcttatg ccgctcggcg cgctctatgg cgcggtcgca
     7201 gcacggcgat tgcagcgcga gggttttgac gccggcgtcc ccgtgctttg cgtcggcaac
     7261 tatcatgtcg gcggcgccgg caagaccccg atggttctgg ccctcaccaa aatactgcgc
     7321 gagctcggcg agacgccgat cgtgctcagt cgcggctatg gcggacaatt gcgcgggccg
     7381 atcaaggtcg atccggagcg gcacgccgct gtcgatgtcg gcgatgagcc gctgatgctg
     7441 gcgcggacgg tcccggtggt ggtcgcgcgc gatcgcatcg gtggcgtcgc tttggcgcgg
     7501 tcgcaaggcg ccagcgtgat cctgatggat gacggctttc agaacccggc gatcgcaaag
     7561 gacgcctccc tgatcgtgat tgacggtgac cgcggcctcg gcaacggcct tgtgtttcca
     7621 gccggtccct tgcgcgcgcc gctgccgccg caacttgtcc gcactgacgc gttgatcgtc
     7681 gtcggcgatg gagccgccgc cgcgccggtt gctgccgcca tcgctgcgct gggcaggccc
     7741 gtgctgtcgg cccatttccg ggctgatgcg tcggcggcgt cgctcaacgg caaacgcgtg
     7801 ctcgcctttg ccgggatcgg cgatcccgtc aggtttttca ggacgctgcg tgccagcggg
     7861 atcgagattg tgcgggagcg agccttcgcc gatcaccatg cgtatacgca ggccgagatc
     7921 gaagccttga tcgctgaggc aaagcgcgat gcgctgatgc tggtgacgac ggaaaaggat
     7981 ctggcgcggc tgcgcatcgg cgggcaactt gcgccttatg cgcagggaat cgagccgttc
     8041 gcggtgacgc tggagttcga tgacgccgcg cagctacgga agttcgtaag cgggcgttta
     8101 ttcaaggcgc gagccaagaa atttcgcgtc aactcgtagg gtgggcaaag cgatagcgtg
     8161 cccaccatcc tgtcgtgctt atcgcgattc ggcgaatggt gggcacggcg caagcgcgcc
     8221 tttgcccacc ctacgatttc acgccttcaa cgcgccggga aaatgccgct gcagcaccgc
     8281 ggttgggatc gcgtaggctt cctgcaggtc gacgctccag tatttcagct cgtcgagcgg
     8341 tatccgtgca tcggtcaccg cgcagcgcac gtaggttccc ggc
//
LOCUS       DASWGM010000122        12324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1393565,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000122 DASWGM010000000
VERSION     DASWGM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12324
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393565"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /gene="fahA"
                     /locus_tag="VGA15_03700"
     CDS             complement(<1..853)
                     /gene="fahA"
                     /locus_tag="VGA15_03700"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEX9586830.1"
                     /translation="MPHPNDPTLRSFIDVDPTSDFPIQNLPYGVFSAKDGLAPRVGVA
                     IGDYVLDLWELEQNGRLDVGDLGVFAAPMLNPFMALGPKLWSRTRARISELLRHDNPD
                     LRDNDALRKRALVPMADARLHLPIAVAGYTDFYSSKEHATNVGVMFRGKDNALQPNWL
                     HMPIGYNGRASTVVVSGTKVRRPHGQLKPPSAELPSFGPCKRLDFELEMGVVIGRPSP
                     MGEMLTEQKAEDMIFGFVLLNDWSARDIQQWEYVPLGPFQAKAFATSISPWIVTREAL
                     EPFRLHGP"
     gene            complement(1086..2432)
                     /gene="hmgA"
                     /locus_tag="VGA15_03705"
     CDS             complement(1086..2432)
                     /gene="hmgA"
                     /locus_tag="VGA15_03705"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX9586831.1"
                     /translation="MNINTSPDVISRTSVNIAPGYMSGFGNSFETEALPGALPIGRNS
                     PQRCAYGLYAEQLSGSPFTAPRGSNERSWLYRIRPSVRHSGRFTRVDAGLWRTAPCFE
                     QELSIAQLRWDPAPIPTQDMTFLQGVQTMTTAGDASAQAGMAAHVYLISQSMVDQHFY
                     NADGEMMFVPQQGSLRFVTEFGRIDAEPGEIVVIPRGVKFRVEITAGPARGYLCENYG
                     GAFTLPERGPIGANCLANARDFLTPVAAYEDKDTPTELFVKWGGALFSTKLPHSPIDV
                     VAWHGNYAPYKYDLRTFSPVGAIGFDHPDPSIFTVLTSPSETAGTANIDLVIFPERWA
                     VAENTFRPPWYHMNIMSEFMGLIYGVYDAKPQGFVPGGISLHNMMLPHGPDREAFDHA
                     SNSELKPVKLTGTMAFMFETRYPQRVTAHAAKSPTLQHDYADCWKGLEKRFDPNRR"
     gene            complement(2435..3388)
                     /locus_tag="VGA15_03710"
     CDS             complement(2435..3388)
                     /locus_tag="VGA15_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9586832.1"
                     /translation="MAKGFASTTDMAEKKITFSEIGPDLYAFTAEGDPNSAVIVGDDG
                     CIVFDAQATPAMANKVIERVRTVTDKPIKYVVLSHYHAVRVLGASAYHAQAVIASAET
                     HRLIEERGQQDWDSEYGRFPRLFQDAQSIPGLTWPTLTFETDMSIYLGKREVRLMSLG
                     AGHTSGDIVAWVPDAEVMFSGDLIEYHSACYCGDAHLREWPATLNEIRAFNPKAIAPG
                     RGDALKGLAIGRDAIAMTRDFVTSLYGAAETAVAKGRNLKETMAATREVMDPKFSDFA
                     IYEHCLPFNVSRAFDEASGVDDPVIWTDKRDQEMWASLQGG"
     gene            complement(3409..3636)
                     /locus_tag="VGA15_03715"
     CDS             complement(3409..3636)
                     /locus_tag="VGA15_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HEX9586833.1"
                     /translation="MSLSTSSNFAKPDDAFRAILEAHRGLNDAQSADLDAALVLILAN
                     HIGDLDVLREAIALARRRMLDASQQQQQQQQ"
     gene            complement(3646..5262)
                     /locus_tag="VGA15_03720"
     CDS             complement(3646..5262)
                     /locus_tag="VGA15_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9586834.1"
                     /translation="MAQQSKTQFGYRRHSDQDRTGGNAAEYPVVVVGAGPVGLSLAID
                     LAQRGQAVVLLDDADRIGEGSRAICFSKRSLEFWDRLGVGGRMVDKGVVWSVGKIFYG
                     ESPLYQFNLLPEQGHKRPAFINLQQFYAEAYLVDRVQELAAIDLRWRNKVIGLEQRND
                     HAALTIETPDGAYRIRADYVVACDGARSSLRQMVGAEFAGKVFEDQFLIADVKMTAEF
                     PTERWFWFDPPFHAGRSALLHRQPDDVWRVDLQLNRYADPAFEKLPENVRPRIARMLG
                     HDKFDFEWISLYKFQCRRMEKFIHGHVIFAGDAAHQVSPFGARGANSGLEDAENLAWK
                     LDRVLRRTSPEALLETYHAERSAAADENIRESTRSTDFMAPNSHQEARLRKAVLSLAK
                     ETEFGKRMVNGGRLSVPSIYDSELSTADGDAWRGGPRPGASMPDAPIAARDRDPAFLT
                     EAFIEAGMRFTLLEFANGAAVDAPDGVGVIRIGGHDGFSDSAGLAGARYDAEPGTAYL
                     LRPDGYVAARFRHPTPAALAAALARAAGIN"
     gene            5371..5847
                     /locus_tag="VGA15_03725"
     CDS             5371..5847
                     /locus_tag="VGA15_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9586835.1"
                     /translation="MAKLDLFRFVPFRLNRLAAEVSAALSSEYQERYGLDIPEWRVLA
                     TLGFREDACSAQYIAHCTRTHKSTISRAVTTLMTRQLVERVENEDDRREFRLRMTRKG
                     KALYEELIPRLKRKEQAILSCLSAQERKDFAHMLGKIETSLDLVQTSREASAKEAY"
     gene            complement(6046..6390)
                     /locus_tag="VGA15_03730"
     CDS             complement(6046..6390)
                     /locus_tag="VGA15_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9586836.1"
                     /translation="MINRFPGLTPTRSRAVVHDGLVFTVAVAPDPVTSSMYEQSASAL
                     ARIDESLALCGSDKSKILSAIVYITDMKRKGEMNLAWDEWVDPRNPPMRACIGVDLEP
                     PHIVEIVVTAAK"
     gene            complement(6447..7253)
                     /locus_tag="VGA15_03735"
     CDS             complement(6447..7253)
                     /locus_tag="VGA15_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX9586837.1"
                     /translation="MGNVYEIYALRYATMSPRTPHLNYLLPDPHETTAQDLDYFVWLI
                     RGGGRDILVDTGFNAEEAKLRSRKLTLNPVEALARFGVNADDIRDVVVTHLHYDHAGN
                     LDRFPNARFHLQDREMSYATGRCMCHGVLRHPFSVEHVTLMVRHVFSDRVTFHSGDGE
                     VAPDVTLHRVGGHSDGLQVVRVETARGPVVLASDASHYYANMHRRSPFPILYNLGDMY
                     EGWDIVARLAGHPDRVIPGHDPIVTEIYPRASEKVDAYSLHMQPSRSFAK"
     gene            7636..9120
                     /locus_tag="VGA15_03740"
     CDS             7636..9120
                     /locus_tag="VGA15_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9586838.1"
                     /translation="MKIRLALVVTVLLSVAPLVPSVAADNRGDRFALVIGNAKYPDAD
                     APLKEPINDARDVADELKRDGFNVEVGENLTGEAMRRAFDKFYARIKPGAVALIFYSG
                     FGVQSGRQSYMIPIDAQIWTEPDVRRDGFSLESVLGEINGRGAGVKIALIDASRRNPF
                     ERRFRSFSAGLAPVIAPNGTLVMYSAALSSVISDNGGDHSPFVQELLKEIRVPDLMAE
                     ETLNRTRVGVTRASRQEQVPWISSSLAEDFSFIPGASPKPPGPVAALPKPAAEPPPLP
                     RIEPPSPRVEPPPPAQKPVEGPSPDKPLADKPVADKPVADKPLEKNDGNTPVLADDPT
                     IKSLTLKLADNPDDAGALYRRGQVYASKGAYTLAIKDFDNSLRLNPKDVEAYNNRCWT
                     RTVVGDLQAALKDCNEALRLRPNFVDALDSRGLVNLKSGQTKNAIADFDAALRINPRL
                     TSSLYGRGLAKQRNGAISEGALDIANAKAMDPNIVKEFADYGVR"
     gene            9271..9933
                     /locus_tag="VGA15_03745"
     CDS             9271..9933
                     /locus_tag="VGA15_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9586839.1"
                     /translation="MSKMLGRKGFGALLSMLAMGAALSFGIGKAVAADDVTADQIVRA
                     LAPAAKAPLTRGLSADPQADPSANAAEGRLLQTIRGRATRSLSVSEREEIATIAKDKP
                     NIDLEITFDYNSADISTKSLPSVQALGKALTNPDLKGSTFIVAGHTDAAGGDAYNQDL
                     SERRADSIKRYLTGKFSIALADLVTVGYGKSKLKDPANPLAEVNRRVQVVNMANKTTA
                     AK"
     gene            10334..11257
                     /locus_tag="VGA15_03750"
     CDS             10334..11257
                     /locus_tag="VGA15_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9586840.1"
                     /translation="MTLSAFLKPVLAVIAVVALGGGWYWFEHRSHPEEKETPSNALVI
                     VTKSTNACFSDLVKVTGFIVPRREAVVGVDQEGSKVADVLVREGETVADNQELARLTP
                     PPAQPGAAGGRPAPTSLRAPAAGLVTEVKTIVGAPASPQSGPMFRISVNNEIELDAEV
                     PAIHVMKLNAGATVRISRDDAPDLVGRVRLVSPQIDRTTQLGHVRISLTNNPSLKVGM
                     FARASIDAKRSCGVAVPRTAIDHLTVQVVKGNIVETRRVRVGLTNDNSTEILEGLDEG
                     EIVVADAGSSLHDGDQIKTMFAEDLDRTRVR"
     gene            11257..>12324
                     /locus_tag="VGA15_03755"
     CDS             11257..>12324
                     /locus_tag="VGA15_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9586841.1"
                     /translation="MALNISAWSIRNPLPSIVFSIILLVLGWVSFTKLAVTRLPSADI
                     PVISVAVAQFGAAPAELESQVTKTIEDGVSGVEGVRHISSSITDGLSLTTIQFALETN
                     TDRALNDVKDAVTRVRSNLPQNVNEPLIQRVDVIGLPIVTYAAISPGKTPEQLSYFVD
                     DVVKRALQGVRNVAQVERIGGVEREILVSLDTDRLQAAGLTAVNVSQSLRGTNVDLAG
                     GRAEIGKNDQAIRTLAGAKTLNELAGTMIPLFGGGEVRLDDLGTVTDTIADRRTFARF
                     NGEPVVALGIKRSKGASDVVVAAAVQKRIDALKIAYPDVDLKLIDTSVEFTKGNYEAA
                     ISTLFEGAILAVIIVLLFLRDI"
BASE COUNT         2357 a         3880 c         3801 g         2286 t
ORIGIN      
        1 tgggcccatg aaggcggaac ggctccagcg cctcgcgcgt cacgatccac gggctgatcg
       61 aggtcgcaaa cgccttggcc tggaacggcc cgagcggcac atattcccac tgctggatgt
      121 cgcgcgcgct ccagtcgttc agcagcacaa agccgaagat catgtcctcg gctttctgtt
      181 cggtaagcat ctcgcccatc ggcgacggcc gtccgatcac gacgcccatt tcgagttcaa
      241 aatcgagccg cttgcagggc ccaaaactcg gcagctccgc actgggcggt tttaattgcc
      301 cgtgcggccg ccgcaccttg gtgcccgaga ccacgacggt ggacgcgcgt ccgttgtagc
      361 cgatcggcat atgcagccag ttcggctgca acgcgttgtc cttgccgcgg aacatgacgc
      421 cgacattggt ggcgtgctcc ttcgacgaat agaaatcggt gtaaccggcg accgcgatcg
      481 gcagatgcag tcttgcgtcg gccattggta ccagcgcgcg tttccgtaac gcgtcgttgt
      541 cgcgcagatc cggattgtca tggcgcagca attcgctgat ccgcgcccga gtacgcgacc
      601 acagcttcgg ccccagcgcc atgaagggat tcagcatcgg cgctgcaaac acaccgagat
      661 cgccgacatc cagccgcccg ttctgttcaa gctcccagag atcgagcacg tagtcgccga
      721 tcgcgacgcc gacgcgcggg gccaacccgt cctttgccga gaacacgccg tagggcaggt
      781 tctggatcgg aaagtcggag gtggggtcga cgtcgatgaa ggagcggagg gtggggtcgt
      841 tgggatgggg cacaatggtt ctccgggctt cattcttact tcccttctcc ccttgtggga
      901 gaaggtggcc ataggcggtc ttcggccgcc gttctttaat aggacgccga tgctttgcat
      961 cggctatgcg aagtccgtgc cggatgaggg gttacggtct atcgatagtc cgagacccct
     1021 cacccgtcgc gaattcgctg cgctcattcg cgccaccctc tcccacaagg ggagagggga
     1081 agagctcacc gccggttcgg atcgaaccgc ttttccagtc ccttccagca atcggcgtaa
     1141 tcatgctgca gcgtcgggga tttcgcggcg tgcgcggtca cacgctgcgg atatcgcgtc
     1201 tcgaacatga aggccatggt gccggtcagc ttgaccggct ttaattcaga attgctggcg
     1261 tgatcgaagg cctcgcggtc ggggccgtgc ggcagcatca tgttatgcag gctgatgccg
     1321 ccggggacaa aaccttgcgg cttggcatcg tagacgccgt agatcagccc catgaactcc
     1381 gacatgatgt tcatgtgata ccacggcgga cggaaggtat tttccgccac cgcccagcgc
     1441 tcggggaaga tcaccaaatc gatgttggcg gtgcccgcgg tttccgacgg cgacgtcaac
     1501 accgtgaaga tcgacgggtc gggatggtcg aaaccgattg cgccgaccgg cgagaaggtg
     1561 cgcagatcgt atttgtacgg cgcgtaattg ccgtgccatg ccaccacatc gatcggcgaa
     1621 tgcggcagtt ttgtcgaaaa cagcgcgccg ccccatttca cgaacaattc ggtcggcgtg
     1681 tccttgtctt cataggcggc aacgggggtg agaaaatcgc gcgcattggc aaggcaattg
     1741 gcacctatcg gtccgcgttc cggcagcgtg aaggcgccac cgtagttttc gcaaagataa
     1801 ccgcgcgccg gaccggctgt gatctcgacg cggaatttta ccccgcgcgg aatcacaacg
     1861 atttcacccg gctcggcatc gatccggccg aactcggtaa cgaaacgcaa gcttccctgt
     1921 tgcggcacga acatcatctc gccgtcggca ttgtagaaat gctgatcgac catcgattgg
     1981 gagatgaggt agacgtgtgc ggccatgcct gcttgcgcgc tggcgtcgcc cgccgtggtc
     2041 atggtctgca cgccctgcag gaaggtcatg tcctgcgtcg ggatcggcgc cggatcccag
     2101 cgcaattgcg cgatcgatag ctcctgttcg aaacacggcg cggtgcgcca caggccggcg
     2161 tcgaccctgg tgaagcgccc ggagtgtctt accgacggcc ggattcgata gagccatgag
     2221 cgttcattgc tgccgcgcgg cgccgtgaac ggcgagccgg acagctgttc ggcatagagc
     2281 ccataggcgc aacgttgcgg cgaattgcgc ccgatcggca gcgcaccggg cagcgcctcg
     2341 gtctcaaagc tgttgccgaa gccggacata tagcccggcg cgatattgac ggaagttcgc
     2401 gaaatgacgt cgggtgacgt gttgatgttc atggtcatcc tccttgcaag ctggcccaca
     2461 tttcctgatc gcgcttgtcg gtccagatca cggggtcgtc cacgcctgaa gcttcgtcga
     2521 acgcgcgcga cacgttgaac ggcagacaat gttcgtagat cgcaaagtcg gagaatttcg
     2581 ggtccattac ctcgcgggtc gccgccatcg tctccttgag gttgcgaccc ttggcgaccg
     2641 ccgtttccgc ggcgccatag agcgaggtaa cgaaatcgcg cgtcatggcg atggcatcgc
     2701 gcccgatagc taagcccttg agggcatcgc cgcgccccgg cgcgattgcc ttgggattga
     2761 aggcgcgaat ttcgttcagc gtcgccggcc attcgcgcaa atgcgcgtcg ccgcaatagc
     2821 aggccgaatg atattcgatc agatcgccgg aaaacatcac ctcggcatcg ggcacccagg
     2881 ccacgatatc gccggaggtg tgccccgcgc cgagcgacat cagccgcact tcgcgtttgc
     2941 cgagatagat cgacatgtcg gtttcgaacg tcagcgtcgg ccaggtcagg cccgggatgc
     3001 tctgggcatc ctggaacagc cgcggaaagc ggccgtattc cgaatcccag tcctgctggc
     3061 cgcgctcttc gatcaggcga tgggtttccg ccgaggcgat caccgcctgg gcgtgatagg
     3121 ccgacgcgcc gagcacgcgc accgcgtgat aatgcgacag caccacatac ttgatcggct
     3181 tgtcggtgac ggtacgcacc cgctcgatca ccttgttggc catcgccggg gtcgcctgcg
     3241 cgtcgaatac gatgcagccg tcgtcgccga cgatcacagc ggaattcgga tcgccttcgg
     3301 cggtgaaggc gtagagatcg gggccgatct cggagaaggt gattttcttc tccgccatgt
     3361 cggtggtgga tgcgaagcct tttgccatca atccaattcc ctttgcactt actgttgttg
     3421 ttgttgctgc tgctggctcg catccagcat ccgccgccgg gcgagcgcga tggcttcgcg
     3481 caatacgtcg agatcgccga tgtgattggc gagaatcaaa accagcgccg cgtcgagatc
     3541 ggcgctctgc gcgtcattca atccgcgatg cgcttcgagg atcgcgcgaa acgcatcatc
     3601 cggcttggcg aaattcgagc tggtggatag cgacatgatg aaacctcagt tgatgccggc
     3661 ggcacgcgcc agcgcggcgg cgagcgcggc gggtgtcggg tgccggaaac gcgcggcgac
     3721 atagccgtca ggccgcagca gataggccgt accgggttcg gcatcgtagc gcgcgccggc
     3781 gaggcctgcg gaatcggaaa agccatcatg accgccgatg cggatgacgc cgacgccatc
     3841 gggagcatcg accgcggcgc cgttggcaaa ctcgagcagc gtgaaccgca ttcccgcttc
     3901 gatgaaggct tcggtcagga atgcaggatc gcgatcacgc gccgcaatcg gcgcgtcggg
     3961 catggaagcg cccggccgcg gaccgccgcg ccatgcatcg ccgtcggcgg tcgaaagctc
     4021 ggagtcatag atcgagggaa ccgacagccg tccgccattg accatgcgct tgccgaattc
     4081 cgtctccttg gccagcgaca gcaccgcctt ccgcagccgc gcctcctgat gggaattggg
     4141 cgccatgaaa tcagtcgaac gggtcgattc ccggatgttc tcgtcggccg ctgcgctgcg
     4201 ctcggcgtga taggtctcga gcaaggcttc cggcgatgtc cttctcagca cccggtcaag
     4261 tttccaggcc agattctcgg cgtcctccag acccgaattg gcgcccctcg caccgaacgg
     4321 tgagacctga tgcgcggcat caccggcaaa gatcacgtgg ccgtggatga atttttccat
     4381 ccgccggcac tggaacttgt agagcgatat ccattcgaaa tcgaacttgt cgtgcccgag
     4441 catgcgcgcg atgcgtggcc gcacattctc cggcagcttt tcgaaagcgg gatccgcata
     4501 ccgattgagt tgcaagtcaa cgcgccagac gtcgtcaggc tgcctgtgca agagcgccga
     4561 ccgtccggcg tgaaacggtg ggtcgaacca gaaccagcgc tcggtcggaa attccgccgt
     4621 catcttgacg tcggcgatca ggaattgatc ctcgaatact ttccctgcga attcggcgcc
     4681 caccatctgc cgcagcgacg accgcgcgcc gtcgcaggcg acgacatagt ctgcgcgtat
     4741 ccggtaagct ccatccggcg tctcgatcgt cagcgccgca tggtcattgc gctgctccag
     4801 cccgatcacc ttgttgcgcc agcgcagatc gatcgcagca agctcctgga cccggtccac
     4861 cagataggct tcggcataaa attgctgcag gttgatgaag gcgggccgtt tgtgtccctg
     4921 ctccggcagc agattgaatt gataaagcgg cgattcgccg tagaaaatct tgccgacgct
     4981 ccacaccacg cccttgtcga ccatgcggcc gccgacgccg agccgatccc agaactccag
     5041 cgagcgcttg gaaaaacaga tcgcgcgcga cccctcgccg atccggtcgg cgtcgtcgag
     5101 cagaacgacg gcttggccgc gctgcgccag atcgatcgca agcgacagac ctaccggccc
     5161 ggcgccgacc accacaacgg gatattcggc cgcgtttccg ccggtccgat cctggtcgga
     5221 atggcggcgg tagccgaact gggttttgga ctgctgcgcc atgtgctcac gtggttctgg
     5281 ccaacggccc ggatcacagc taggatagtc gcatctgcaa ccatctaaac tcccctcgcg
     5341 ttgccccgtc aactcaaatt ggagcggatt ttggccaaac ttgatctgtt ccgatttgtg
     5401 ccattccgtc tgaaccggct ggcggccgag gtctcagccg cgctgtcgag cgaatatcag
     5461 gaacgctacg ggctggacat tccggaatgg cgcgtgctgg cgacgctcgg cttccgcgaa
     5521 gacgcctgca gcgcgcaata cattgcgcat tgcacacgga cgcacaaatc gaccatcagc
     5581 cgcgcggtca cgacgttgat gacacggcaa ctggtggagc gtgtcgaaaa tgaagacgac
     5641 cgccgtgaat tccgcctgcg catgacgcgc aagggcaaag cgctctatga ggagctgatc
     5701 ccacgcttaa agcgcaagga gcaggcgatc ctgtcgtgcc tctccgcgca ggagcgcaag
     5761 gattttgcgc acatgctcgg caagatcgaa acgagcctcg atctggtgca gacgagcagg
     5821 gaggcgagcg ccaaggaagc gtattgaagg caactatcgt cattccccga tgtgcaattg
     5881 cacatctgag gatgcgccgc ttggcgctgg cccggaatcc atactcccgg cggtggttat
     5941 ggattccggg ctcgcgactt cgtcgcgccc cggaatgacg gttacgcccc gtcgatatgc
     6001 ttcacgatca ccgcgaaata ggccgcccgc acaaccagcg accgctcact tcgctgctgt
     6061 caccacgatc tccacgatgt gcggcggttc gagatctaca ccgatgcaag cccgcatcgg
     6121 tggatttctg gggtcgaccc attcgtccca ggcgagattc atctcgccct tccgcttcat
     6181 gtcggtgatg tagacgatgg ccgagaggat cttgctcttg tccgagccgc acagcgcgag
     6241 gctctcgtcg atgcgggcaa gcgcacttgc gctctgctcg tacatcgagg aggtaaccgg
     6301 atcgggcgcg accgccacgg tgaacacaag accgtcgtga acgacggcgc ggctgcgggt
     6361 tggcgtgagg ccaggaaagc gattgatcat ggctcgctcc ggttggatga aggttccgtc
     6421 atccgcatct ttttggcccg ctttcgctat tttgcgaacg agcgggatgg ttgcatatgc
     6481 aacgaatagg catcgacctt ttcgctggcg cgcggataga tttccgtgac gatcggatcg
     6541 tgcccgggga tgacgcggtc gggatggccg gccagccgcg ccacgatgtc ccatccctca
     6601 tacatgtcgc cgagattgta gaggatcgga aacggactgc ggcggtgcat gttggcgtag
     6661 tagtgcgagg cgtccgaggc aagcaccacc ggtccgcggg cggtctcgac ccgcaccact
     6721 tgcagcccgt cggaatgacc gccgacgcga tgcaacgtca cgtcgggtgc gacctcgcca
     6781 tcgccggaat gaaaggtgac gcggtcgctg aaaacgtggc ggaccatcag cgtgacgtgc
     6841 tccaccgaga acggatggcg cagcacgcca tggcacatgc agcgtccggt ggcgtagctc
     6901 atctcgcgat cctgcagatg gaagcgcgcg ttgggaaagc ggtcgagatt gccggcgtga
     6961 tcgtaatgca gatgcgtgac cacgacatcc ctgatgtcgt cagcattgac gccaaaacgc
     7021 gccagcgctt cgaccggatt aagcgtgagc ttgcggctgc gcagcttcgc ttcctcggcg
     7081 ttgaaaccgg tatcgaccag aatgtcgcgg ccgccgcccc ggatcagcca gacgaaatag
     7141 tcgaggtctt gcgccgtggt ctcgtgcggg tccggcagca ggtaattgag gtggggcgtg
     7201 cgtggcgaca tcgtcgcata gcggagcgca tagatttcgt agacgtttcc catcggattt
     7261 ttacttcttc ttaggtgctt cttaggtgcg cgtttggccg attttcccac caagccaccg
     7321 tcatccgaaa aggtcaaaca cggcgcggcg catggcagat tcatgacgta atgaggcatc
     7381 taatgtgagg ctaatcacat tgctatgtcg agggaacctg tatcatcgcc gggagattag
     7441 gtggcgagag aaaatcccga acgcgattta ttccggcgcc ttcggtgatc cttgtcgcgg
     7501 tcaattgtcg caattagcgg tggcctcgca agcggttgac ggttcgggcg cccggtttga
     7561 taatgcagtt tgcgtagagt aaggtcgccg aggcgcaagc tggtatcggc gccctgttcg
     7621 ctggaccgct gtcgtatgaa aatccgtctc gctttggttg tgaccgtgct gttgtcggtc
     7681 gcgccgcttg tcccgtcggt tgctgcggac aatcgcggcg accggtttgc gcttgtcatc
     7741 ggcaacgcca aatatcccga cgccgatgcg ccgctcaagg agcccatcaa cgacgcccgc
     7801 gacgtcgccg acgaactcaa gcgcgacggc ttcaatgtcg aggtcggaga gaacctgacc
     7861 ggcgaggcga tgcgccgcgc tttcgacaaa ttctacgcac gtatcaagcc cggcgcggtg
     7921 gcgctgattt tctatagcgg gttcggcgtt cagtccggcc gccagagtta tatgataccg
     7981 atcgatgccc aaatctggac cgagccggat gttcgccgcg acggcttcag cctggaatcg
     8041 gtgctgggcg aaatcaacgg ccgcggcgcg ggcgtcaaga tcgccctgat cgacgcctct
     8101 aggcgcaatc ccttcgagcg ccggttccgc agtttctcgg ccggccttgc accggtcatc
     8161 gcgccgaacg gaacgctggt gatgtattcg gcggcactgt cttccgtcat ctcggacaat
     8221 ggcggcgatc acagcccgtt cgtgcaggag ctgctcaagg agatccgcgt tcccgacctg
     8281 atggccgaag agacgctcaa tcgaacccgg gtcggcgtca cccgcgcatc gcgccaggag
     8341 caggtgccgt ggatttcgtc ctctttggct gaggattttt cgtttatccc gggcgccagc
     8401 ccgaagccac ccggcccggt ggcggcgctt cccaaaccgg ctgccgaacc cccgccgctg
     8461 ccgcgcatcg aaccgccatc gccgcgcgtc gaaccgccgc cgcctgcgca aaaaccggtt
     8521 gaaggtcctt cgcctgacaa acctttggcg gacaagcctg tggccgacaa gcctgtggcc
     8581 gacaagccgc tggaaaaaaa tgacggaaac acgccggtgc ttgccgacga tccgaccatc
     8641 aaaagcctga ccttaaaact cgccgacaat cccgacgacg ccggcgcgct gtaccggcgc
     8701 ggacaggtct atgccagcaa gggggcctac acccttgcca tcaaggattt tgacaactcg
     8761 ctgcgattga accccaagga cgtcgaagcc tacaacaacc gctgctggac ccgcaccgtg
     8821 gtcggcgacc tgcaggcggc attgaaagac tgcaacgagg cgctgcggct gcgcccgaac
     8881 tttgtcgatg ccctcgacag ccgcgggctg gtcaatctga agagcgggca gaccaaaaac
     8941 gcgattgccg attttgacgc cgccctcagg atcaatccga gattgacctc ctcgctgtac
     9001 ggacgcggcc ttgccaagca gcgcaacggc gcgatttcgg aaggcgcact ggacatagcc
     9061 aacgccaagg cgatggatcc gaatattgtg aaggaatttg ccgactacgg ggtgcgttga
     9121 cgaaaattta ggttgatcga cccggtgctt tccctgaacc ccggagcggg cgccagagac
     9181 aaatcaggga agcatggccc acgaccgatg ggtccggtgg ccgatttgca aatgatttgg
     9241 aaccgcgcgg gtaacgcagg aggactgaca atgtcgaaaa tgttgggaag gaagggtttt
     9301 ggcgcgcttc tttcgatgct ggcgatgggc gctgcacttt catttggcat aggaaaggcc
     9361 gtcgcagccg atgacgtcac cgcggatcag atcgtccggg cgctggcacc ggcggccaag
     9421 gcgccgctga cccggggcct gtcggccgat ccgcaggccg atccgtcggc caacgccgcc
     9481 gagggtcgtc tgttgcagac catccgcggc cgcgccaccc gttcgctctc ggtcagcgag
     9541 cgcgaggaaa tcgccaccat cgccaaggac aagccgaata tcgatctgga aatcaccttc
     9601 gactacaatt cagccgatat cagcacaaaa tcattgccct cggtccaggc gctcggtaag
     9661 gccctgacca atcccgatct gaagggctcg accttcatcg tcgccggtca caccgacgcc
     9721 gccggcggcg acgcctacaa tcaggacctg tccgagcggc gcgcggattc gatcaagcgc
     9781 tatctcaccg gcaaattctc gatcgccctc gccgacctcg tgaccgtcgg ctatggcaag
     9841 agcaagctca aggacccggc caacccgctg gccgaagtga accgccgcgt ccaggtcgtc
     9901 aacatggcca acaagaccac ggcggcgaag tagccttcat ccccgcggac cggcttcgcc
     9961 gttcagctcg tcatgcccgg ccttgtgccg ggcatccacg tcttgacggt gcagaaacaa
    10021 gaatggcgag gagcgaagca acgaagcaat ccagactccg tcattccggg gcgatgccat
    10081 cgggcccgcg ccaagcgcgg cccgatgaca ggctccattg aacccggaat ccaaggattc
    10141 cccgatgtgc agtgcacatc tcagctctgg tgctaacgca ccatccacga tacgcaattg
    10201 cgcatcgggg aatgacggag tctggattgc ttcgctgcgc tcgcaatgac aatctcagac
    10261 catcttagac ccatcttaat ggctgcattt tccggtcata cttacgcgac catagctctg
    10321 gattgatccc gtaatgacgc tctccgcgtt cctcaagcct gtactggccg tgatcgccgt
    10381 cgtcgccctc ggcggcggct ggtactggtt tgagcaccgc tcgcatcctg aggagaagga
    10441 aacgccgagc aacgcgctgg tcatcgtgac gaaatccacc aatgcctgtt tctccgatct
    10501 ggtgaaggtc accggattta tcgtgccgcg ccgcgaagcg gtggtcgggg tcgaccagga
    10561 aggctccaag gttgccgacg tgctggtgcg cgaaggcgag acggtcgccg acaatcagga
    10621 actggcgcgg ctgacgccgc cgccggcgca gcccggcgcc gccggcgggc gacccgcgcc
    10681 gacgtcgttg cgcgccccag ccgcgggcct cgtcaccgag gtgaaaacca ttgtcggcgc
    10741 cccggcctcg ccgcagtccg ggccgatgtt ccggatttcc gtcaacaacg aaatcgaact
    10801 cgatgccgaa gtgccggcga tccacgtcat gaaactcaat gcgggcgcga cagtgcgcat
    10861 cagccgcgac gacgcgcccg acctggtggg gcgggtgcgg ctggtatcgc cgcagatcga
    10921 ccgcaccacc cagcttggcc atgtccggat atcgctgacc aacaacccct cgctgaaggt
    10981 cggcatgttc gcccgcgcca gcatcgacgc caagcgcagt tgcggcgtcg ccgttccccg
    11041 caccgccatt gaccatctga ccgttcaggt ggtcaaagga aatattgtgg agacgcgaag
    11101 ggtgcgggtc gggctcacca acgacaactc cacggaaatt ctcgagggcc tcgacgaggg
    11161 cgagatcgtc gtggccgatg ctggctcctc gctgcatgac ggcgaccaga tcaagaccat
    11221 gttcgccgag gacctcgatc gaacgcgggt acgctgatgg ctttgaatat ctcggcatgg
    11281 tcgatccgga acccgcttcc ctcgatcgta ttctccatca tcctcttggt gctgggctgg
    11341 gtcagcttta ccaaactcgc ggtaactcgg ctgccctcgg ccgacatccc cgtgatctcg
    11401 gtcgcggtcg cgcagttcgg cgcggcccct gccgaacttg aatcgcaggt caccaagacc
    11461 atcgaagacg gcgtctccgg cgtcgaaggc gtgcggcaca tttcatcgtc gatcaccgac
    11521 gggctgtcgc tgaccacgat tcagttcgca ctcgagacca acaccgaccg cgcgctgaac
    11581 gacgtcaagg acgccgtcac ccgggtgcgc tccaatctgc cgcagaacgt caacgagccg
    11641 ctgatccagc gcgtcgacgt gatcggcctg ccgatcgtca cctatgccgc gatctcgccc
    11701 ggcaagacgc ccgagcaact ttcctatttc gtcgacgacg tcgtcaagcg cgcgctgcag
    11761 ggcgtgcgca acgtcgccca ggtcgagcgt atcggcggtg tcgagcgcga gattctggtg
    11821 tcgctcgata ccgaccggct gcaggccgcg gggctcaccg ccgtcaatgt cagccagagc
    11881 ctgcgcggca ccaatgtcga tctggccggc ggccgcgccg aaatcggcaa gaacgatcag
    11941 gcgatccgca cactggccgg cgccaagacg ctcaatgaac tggcggggac catgatcccg
    12001 ctgttcggcg gcggcgaggt gcggctcgac gatctcggca ccgtcaccga caccatcgcc
    12061 gaccgcagga catttgcgcg tttcaacggc gagccggtgg tggcgctcgg catcaagcgc
    12121 tccaagggcg ccagcgacgt ggtggtggcg gcggccgtgc agaagcgcat cgacgcgctc
    12181 aagatcgcct atcccgatgt cgacctgaag ctgatcgaca cctcggtgga attcaccaag
    12241 ggcaactacg aagccgcgat ctcgaccttg ttcgaaggcg ccattctcgc cgtcatcatc
    12301 gtgctgttgt tcctgcgcga catc
//
LOCUS       DASWGM010000123         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1393697,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000123 DASWGM010000000
VERSION     DASWGM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393697"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VGA15_03760"
     CDS             complement(<1..1521)
                     /locus_tag="VGA15_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX9586842.1"
                     /translation="MSHVSMRIGVLHFSHETVTFLKNDTTLEDFIYPGSPAKGEALLK
                     FDPKSYMGGFVKMAREFDGVELIGIESPLWPKTSIGSGWVTRDAYETFVGRMIAEINA
                     SEQFKGIYLCLHGAMAVRGVPRPEAELARRVREAVGPNVFIAATFDLHGNEDDAFLTY
                     ADMAFTVKYFPHYDGYLQGERAARTLLRAIRGDYKPAHVTLKVPIISPTVLQWTGASP
                     WMDLVQRALIWEAREVDVYVNVFFGFPFADVPDVGMTIQVLTNGNPELAAKISRDLAE
                     TAWRQREALLTSTKIHTIPEGVALAKQAVQSGKTPVVLADHSDRSGAATWLLREIIAQ
                     DLSNTVIATVTDAAATAKLRAQGAKAGDAFDMEVGGLADPSAGEPVRIQGTILKAVEG
                     YGQFWVSIKFGRNNVLILSTYLVQIVEPFSLKDLDLDVSAFQIMAIKSRVHFRRGFHD
                     NGFAKTILLVEPTEPFLGTVRLDKLPYENVDLKQYYPYGNPAFPGQGLSAATRRVDD"
     gene            complement(1610..2341)
                     /locus_tag="VGA15_03765"
     CDS             complement(1610..2341)
                     /locus_tag="VGA15_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9586843.1"
                     /translation="MDGSPFELPLFLLATFAGALVAGLSGFAFGLVAASIWLYILSPL
                     QTATLIIAFGLIVQGYSVWKLRGALDWRKLWPFVAGAALGVPVGVTILTWANPAHARM
                     GVGAFLVLYSLYALFRPTIDPVKAGGAAADAGVGFLNGVFGGITGLAGILVTIWCGLR
                     GWPKDVQRAVFQPVAVATFLMSALWIGAKGAITAETTKLFLIGLPALLAGTWLGLKLF
                     GRLNEAAFRKIVLALLLASGVVLIV"
     gene            complement(2486..2635)
                     /locus_tag="VGA15_03770"
     CDS             complement(2486..2635)
                     /locus_tag="VGA15_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586844.1"
                     /translation="MRWAITPPQSYVVYLVALFLVAGSSFYIGSLRPKKPMGLGPPPI
                     SAPRN"
     gene            complement(2741..>2902)
                     /locus_tag="VGA15_03775"
     CDS             complement(2741..>2902)
                     /locus_tag="VGA15_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9586845.1"
                     /translation="LIGAPARMAQAAAPLAFGLLIDTMGSRVLIVSSALSLSALLALS
                     LLRKRPAAP"
BASE COUNT          596 a          893 c          898 g          515 t
ORIGIN      
        1 gtcatcgacc cgacgcgttg cggcgcttaa gccttggccg ggaaacgccg ggtttccgta
       61 ggggtaatac tgcttcaggt cgacgttctc ataggggagt ttgtccaggc gaaccgttcc
      121 gagaaacggc tcggtcggct cgaccagcag gatcgtcttg gcaaaaccgt tgtcgtggaa
      181 gccgcgcctg aaatgaaccc gcgacttgat cgccatgatc tgaaaggcgc tgacgtccag
      241 atccagatcc ttgagagaaa acggttcgac gatctgcacc agatacgtgc tcaggatcag
      301 gacgttgttg cggccaaact tgatgctgac ccagaactga ccatagccct cgacggcttt
      361 caggatggtg ccctggatgc gcaccggttc gccggcggat gggtcggcga ggccgcccac
      421 ctccatatcg aatgcgtcgc cggccttggc gccctgcgcc cgcaatttgg cagtggccgc
      481 ggcgtccgtg acggtggcaa tcacggtgtt ggacaggtcc tgcgcgatga tctcccggag
      541 cagccaggtc gcagcgccgg agcggtcgct gtgatcggcc aacacgaccg gcgtttttcc
      601 actttgcacg gcttgttttg ccagagcgac gccttcggga atggtgtgaa ttttggtcga
      661 ggtcagcagc gcttcgcgtt gacgccacgc cgtctccgcc aggtcgcggg atatcttcgc
      721 ggcaagttcg gggtttccgt tggtcagcac ttgtatcgtc atgcccacat cgggcacgtc
      781 ggcaaacgga aaaccgaaga acacattgac gtagacgtcc acttcgcggg cttcccagat
      841 cagcgcccgt tgcaccagat ccatccatgg cgatgcgccc gtccactgca ggacggtcgg
      901 cgagatgatg gggactttca acgtcacatg cgcaggcttg tagtcgcccc tgatcgcccg
      961 cagcagggtt cgcgccgcgc gctctccctg caggtagccg tcatagtgcg gaaaatattt
     1021 tacggtaaac gccatgtcgg cgtaggtgag aaacgcgtcg tcctcattgc cgtggagatc
     1081 gaaggtcgcg gcgatgaata cgttggggcc gaccgcttcc cgcacccggc gcgctagttc
     1141 ggcctcgggg cgaggcacgc cgcgtaccgc catcgccccg tgcaggcaga gatagatgcc
     1201 cttgaactgt tcgctggcgt tgatttcggc gatcattctg ccgacgaagg tttcataggc
     1261 atcgcgcgtg acccatccgg agccgatcga ggttttcggc cacaatggcg actcgatgcc
     1321 aatcagttcg accccatcga attcccgcgc catcttcaca aagccgccca tgtacgactt
     1381 tggatcgaat ttcagcaaag cttcgccctt ggccggtgaa cccggataga taaagtcttc
     1441 gagcgtcgta tcgtttttga gaaacgtcac cgtctcgtgg gaaaaatgca gcacgccgat
     1501 acgcatcgaa acatgactca tgaccgtctg ctctccacat ggaccattcc gcacctcgct
     1561 gcggtgaacg ctcccgagag gtcattgtgg catcgaagaa cgctcgcgcc tagacgatca
     1621 gcacgacccc cgaggcgaga agcagcgcca gcacaatttt tcggaacgcc gcctcgttga
     1681 ggcggccaaa cagtttcaga ccaagccagg tgccggcgag cagggcgggt agcccgatca
     1741 agaacagttt tgtcgtttcc gccgtgatcg cgcccttggc gccgatccaa agcgcgctca
     1801 tcagaaaggt tgcgacggcc accggctgaa acaccgcgcg ctgcacatcc ttgggccagc
     1861 cgcgcaggcc gcaccagatg gtgacgagaa ttccggcaag gccagtgatg ccgccgaaca
     1921 cgccgttgag gaagccgacg ccggcgtcag ccgccgcacc gccggccttg accggatcaa
     1981 tcgtgggacg aaacagcgca taaaggctgt agagcaccag aaacgcgccg acgcccatgc
     2041 gcgcatgcgc cgggttggcc caggtcagga tggtaacgcc gacgggcacg ccgagcgccg
     2101 cacccgcgac aaacggccag agctttctcc agtccagcgc gccgcgcagt ttccagaccg
     2161 aatagccctg cacgatcaag ccgaacgcga tgatcagcgt ggcggtctgc aggggactga
     2221 ggatgtagag ccagatcgag gctgcgacca ggccgaaggc gaagccggaa aggcccgcga
     2281 ccagcgcgcc ggcaaaggtc gccaacagaa acagcggaag ttcgaaaggt gatccatcca
     2341 tgtgctcgct ccatcaaagg gcgagcagcg cttgaatggg atcaccattt catggggagg
     2401 ctgcgatgtc cccgagacag gctacgccgg cagtggccgc gcatgcaagc tgcatatccg
     2461 cgtcctgcag ccgcgatggc aaatttcagt tgcgcggtgc ggagatcggc ggaggaccga
     2521 ggcccatcgg cttcttgggc cgcaggctgc cgatgtagaa cgacgatccg gcgaccagga
     2581 acagagccac gagatacacc acataggatt gcggtggcgt gatcgcccat cgcatcaaac
     2641 ctctaatgac agaagctgga ccagtctcgt tttccatgcc gtgcttttgc tcgaaatggt
     2701 gctgctttgg aagggcaaat gcgttcggtt gcgaaagaag tcatggcgcc gcgggccgct
     2761 tccgcagcag ggacagcgcc agcaaggctg acagactgag cgccgaggag acgatcagca
     2821 cgcggctgcc catcgtgtcg atcaacagac cgaacgccaa tggtgctgcg gcctgcgcca
     2881 tccgcgccgg cgcgccgatc ag
//
LOCUS       DASWGM010000124        12199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1812456,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000124 DASWGM010000000
VERSION     DASWGM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12199
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812456"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VGA15_03780"
     CDS             complement(<1..582)
                     /locus_tag="VGA15_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEX9586846.1"
                     /translation="MRWCVSIGAVLVAAAVAGGDVAGIAAPSPQSQQTESKDNLPSVD
                     IELVIAVDVSYSMDLDELAVQREGYAQAIVSKDFLQAMKNGPNRKVAVTYFEWSASSD
                     QKIIIPWRLIDGPESADAIAAEIMRTPVRRGSRTSISGAIDFAMPLFEENPYRGLRRV
                     IDISGDGPNNNGAPVTGARDAALAKGITINGLPI"
     gene            861..1139
                     /locus_tag="VGA15_03785"
     CDS             861..1139
                     /locus_tag="VGA15_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HEX9586847.1"
                     /translation="MAHTIREKKKLLARVRRIRGQVEAIERALDEEAECERIMHMIAG
                     IRGGVAGLMAEVVEDHIRTHLVDPEKNPGALNADAADQLIEVVHTYLK"
     gene            1148..2422
                     /gene="dmeF"
                     /locus_tag="VGA15_03790"
     CDS             1148..2422
                     /gene="dmeF"
                     /locus_tag="VGA15_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEX9586848.1"
                     /translation="MTNAESIFQFPPHDHVFLGEDHDKAERRTWAVIILCSVMMIAEI
                     IGGALFGSLALIADGLHMSTHAGALLLAALAYTYARKYANDRAFTFGTGKFGDLAGYS
                     SAIVLAMIALLIGYEAVSRLLNPVPISFNEAIPIAVLGLVVNVASAWLLSGGHHHGHS
                     HGHSHGHDHDHHGDEHRRIELHTGAVEIEVFEDNVPPRFRVRADTGVLLAASDVTIET
                     IRPDGGRQLFAFEDRGGYLESRDEIPEPHAFLAKFRLVQGGRHHDHELEFEEHDHEAA
                     HGAHHRDNNMRAAVIHVMADAAVSVLVIVGLLLARAFGWLWMDPLAGFIGALVIANWS
                     VGLLRDTGGILLDRTPDPRVAEKVRMVIESEGDQVTDLHLWRLGPGHLGVIVSVATSG
                     AREAAHYRQRLAKFADLSHVTVEVQHCHSTNG"
     gene            2673..4637
                     /locus_tag="VGA15_03795"
     CDS             2673..4637
                     /locus_tag="VGA15_03795"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX9586849.1"
                     /translation="MMLAGVGPTNSADYSVSRPTKSLAPVPFLNWTGFYVGGHLGYGR
                     GHASGSLTDAVPTISSNSFGSLFGGLQLGYNHLLTSGFLVGVESDVSFANFLGADDIV
                     RSRFTAQGNLAEKIDYIARLRGRFGYASGDWLFYGTGGFAWSQARFIETPGLIANEDK
                     VLRTRPGWVLGAGAEVAIAPDWSARLEYLYDNFGRVDATLPSGRRSESTFDMHTLRLG
                     LNWHLGRAASESPSIENRAYPVIAPDNWNVHGQYTFVGQGYPSFHSPYEGPNSLAGSK
                     QAQNTMSATAFVGLRLWEGAEFYINPELMQGFGLSNVHGLAGFSNGEAQKSSFALPRM
                     NVARIFIRQTFGLGGEQETISDGPNQLAGKQDISRITVTAGKFAVTDAFDGNVYANDP
                     RTTFLNWNIYGGGSYDWTMDKLSWTWGAFVDFNQKDWAFRTGYFLLPVVSNSNNFDTH
                     IPDRGQYTAELELRYSLFSQPGKLRFFGWLNHGTMGGYSDALALPLTSPDYPDITLTR
                     RIRTNYGLVANIEQAITPDLGMFSRATWSPGRTEILGWTDCSESLSFGTVLNGTSWGR
                     PDDKIGVAGVVEGLSAESRAYFAAGGLGILIGDGQLNYRREQILEAYYAYSLNKWATL
                     TFDYQFIGNPGYNADRGPVSVFSGRFHAEF"
     gene            5202..7187
                     /locus_tag="VGA15_03800"
     CDS             5202..7187
                     /locus_tag="VGA15_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX9586850.1"
                     /translation="MIRSSRSRGCVFAGLGLSLLVVDGSAIAADMPVKAPYIRSVFDW
                     TGLYVGAHAGFGRGSSTAVLADPATSTTSNSFSGMIGGVQAGYNYQLPSGMLLGVEAD
                     FSFPNYLTSNSVVSSLATARSDVTEQWDYVGTARGRVGYVAGPWLVYATGGLAWAGER
                     FLNTPAIGSDEKTLHTRLGWAAGGGVEYAFAPHWSLRLEYLYSQFGNADIKFPSATQY
                     TSGLDFQSLRIGLNRKIDWPGSGNFSPASLTDPESDRWEIHGQSTYLPQGYPGFRAPY
                     SGPNSLTPAPQVQATWSNSLFLNARLWEGGEVYYNPELLQGFGLNDTVGAAGFPSGES
                     QKSNFPYPHYNTSRLFLRQTFGFGGEQEELASGPGQLAGKADVSRLTLQVGKFAVTDV
                     FDGNAYAKDTRKDFMNWSMWAPGAFDYGADKLGLTYGATAELNQKQWALRAGYFLMDA
                     VSNSNSFDTNLFQRGEYVVELETRYSLFNQPGKLRTIAWLNSAFSGSYRETLDNPALN
                     LDIAQTRIGRIKYGYVINLEQALTDEVGLFGRWSWNDGKTEIMAFTDIDASLSLGVSI
                     KGTRWGRPDDVIGIGGAINALSRDHRDFIAAGGLGPLIGDGQLNYQPERILESYYAYA
                     LNKQLTLTGDYQLITNPAYNADRGPVHVFSGRLHGEF"
     gene            complement(7246..8079)
                     /locus_tag="VGA15_03805"
     CDS             complement(7246..8079)
                     /locus_tag="VGA15_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX9586851.1"
                     /translation="MPPAGCPSIKVLVHRRTLQICLLSAFAVLGAVDAQAEGCPTATD
                     QIATDRPDVTNSSLVVPVGSLQSENGVNVSARDGGRAIDGTNTRWRLGVAPCLELLLD
                     LPTYFANTGRPGSSGFSDVAPAIKWQVSPIPGKVDLSVVFGVALPTGAVDIAGRGAQP
                     YLQLPWSWELNESWGVSGMLTEFFRPSDVTTKRMTEATFAIEKKVTEKASLFVEYVGD
                     YPDNAGPSQLLNSGGLYHLSPTQQVDFHVAFGLNHNAPSYIVGIGYSFRFDGLFAGKP
                     R"
     gene            8312..10048
                     /locus_tag="VGA15_03810"
     CDS             8312..10048
                     /locus_tag="VGA15_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9586852.1"
                     /translation="MASQQLQQPTLGQALQPNIWDVVALILVVGAMVLIVYGGEQTAL
                     PLSALDVTPVSLDPANLPVYALRTTMRMLLAIVCSIIFTFIYAALAAKSRRAEMVLIP
                     LLDILQSVPILGFLTFTVVFFLNLFPGRVFGAELACVFAIFTSQAWNMTFSMYQSMRN
                     VPKDLEEASQSFHLGGWQRFWRLDVPFAMPGLIWNTMMSMSGGWFFVVASEAITVGNT
                     TVTLPGIGSYVALAIKEQNLPAIGYAILAVFLVILAYDQLLFRPVVAWADKFRFEQTA
                     SASAPTSWMLDLFRRTRALRAMTYPFAALNKAVSNLHIALPSQLKMATRARAQPSRVV
                     DAVWLATIIAGTGYAAWRIYTYLSATLSPSDVSSAVGYGFITLARVIVLIALATLIWV
                     PVGVWIGLRPKLAERIQPLAQFLAAFPANLAFPVFVVIIVRYGLNANVWLSPLMILGT
                     QWYILFNVIAGASAFPTDLREAAGSFHLKGWRWWIKVILPGIFPYYITGAITASGGSW
                     NASIVAEVASWGDTHLTATGLGAYIATATEAGDFPRVVLGIAIMCILVTLFNRLLWRP
                     LYAFGERRLRLG"
     gene            10062..11372
                     /locus_tag="VGA15_03815"
     CDS             10062..11372
                     /locus_tag="VGA15_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9586853.1"
                     /translation="MLDHSNQTNLLDIRGVCRSFPKGSGEDLLVLEKVDLTIHSGEIV
                     GLLGRSGSGKSTLLRIIAGLITPSSGDAKCRGEIIMGPPNGVSMVFQSFALFPWLTVL
                     QNVELGLEALGIDSAVRRTRALAAIDLIGLDGFESAYPKELSGGMRQRVGFARALVVH
                     PDLLLMDEPFSALDVLTAETLRTDLIDLWIEGRLPIKSVLMVTHNIEEAVLMCDRILV
                     FSSNPGRVAAEIKVDLPHPRNRLDPTFRQLVDSIYARMTQRAEVKTPAVEGIHGTGVG
                     MILNHVSSNVLSGLIETLAGPPYSGHADLPVLAGSLQLEADEIFHLGESLQLLRFAQL
                     SEGDLMLTDAGKRFAHLETDARKKLFAEHLINYVPVMDLIRRVLDERPSHTAPVARFR
                     NELEDYMAEDQADETLRTIVSWARYAELFAYDEQSELFSLENPH"
     gene            complement(11405..>12199)
                     /locus_tag="VGA15_03820"
     CDS             complement(11405..>12199)
                     /locus_tag="VGA15_03820"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX9586854.1"
                     /translation="GVAMDEDCISPATHRAFSTPLEMMAFIAEMRRLSGGKPAGFKLC
                     IGHPWEFLAICKAMVQSGIYPDFIVVDGNEGGTGAAPLEFMDHLGMPMREGVNFVHNA
                     LIGINARDRIRIGAAGKIATAFDMARAMAIGADWCNSARGFMFSLGCIQSLSCHTDRC
                     PTGVTTQDPSRARALVVPHKIERVYNYHRATLHALAELIAAAGLEHPQQIRAIHFSQR
                     SSTTEVRSFAQLYPSLRPGELIDGTSDPRYREAWAMARPETFAAAV"
BASE COUNT         2368 a         3734 c         3555 g         2542 t
ORIGIN      
        1 gatcggcagg ccgttgatgg tgatgccctt cgccagcgcc gcgtcgcgtg cgccggtcac
       61 cggtgctccg ttattattgg ggccgtcgcc ggaaatatcg atgacgcgcc ttaggccccg
      121 ataggggttt tcttcgaaca atggcatcgc gaaatcgatc gcaccggaaa tcgaagtgcg
      181 cgagccccga cgaaccggcg ttctcatgat ttcggcagcg atggcgtcgg ccgattccgg
      241 gccgtcaatc agccgccagg gaatgatgat cttctggtcg ctcgatgccg accactcgaa
      301 atacgtcacc gccaccttgc ggttcggacc gtttttcatc gcctgaagga aatctttcga
      361 gacgatcgcc tgtgcatagc cctcgcgctg aacggcaagt tcgtccaggt ccatcgaata
      421 ggaaacgtcg acggcgataa cgagttcgat atcgacggag ggcaaattgt ccttgctctc
      481 ggtctgctgc gattgcgggc tcggcgcggc aatgcctgcg acgtcacccc cggctaccgc
      541 tgctgcgacc agcaccgccc cgatcgagac acaccagcgc acggcaaccc tcccgtcgtt
      601 tcaaggggat ggtgacacgc atgatgcgct gcgcaaagca ggaacccgtg ttttgcttca
      661 cattccagtt agtcagaatt tacagacagc gcggaatcgt tagacgaacg ccagaccgat
      721 ccgcttttcc ttgcgggtgg gtcgactggc cgcggcggct gcatccgcgc tttatctgtt
      781 cggcgggtga agcggttttc gcgatcatcg ccatcaaccg cggttgacgt agcatagggg
      841 ggtaccctat atgaggcgcg atggcacata cgatcagaga gaagaaaaag ctgctcgccc
      901 gggtccgtcg cattcgcggc caggtcgaag cgatcgagcg tgcgctagac gaagaggccg
      961 aatgcgagcg catcatgcat atgatcgcgg gcattcgcgg tggcgtcgca ggcctgatgg
     1021 ccgaagtggt tgaggatcac attcgcaccc atctcgtcga tcccgagaag aatccgggcg
     1081 cccttaacgc cgacgcggcg gaccaactca tcgaagtggt ccacacctat ctcaagtgag
     1141 ggacagcatg accaacgcag aatcgatctt tcagtttccg ccgcacgacc acgtgtttct
     1201 gggcgaggat cacgacaagg cggagcgccg aacctgggcg gtgatcatcc tttgcagcgt
     1261 catgatgatc gctgaaatta tcggcggtgc gctgttcggc tcgttggcac tgattgccga
     1321 cggattgcac atgtcgaccc acgccggggc gctgctgctg gccgcgctgg cttataccta
     1381 tgcccgcaaa tatgccaacg atcgggcttt cacgttcggc accggcaagt tcggcgatct
     1441 ggcaggatac tcaagcgcca tcgttctggc gatgattgcc ctcttgatcg gctatgaggc
     1501 cgtgtcgcgg ctgctcaatc cggttccgat cagcttcaac gaggcgatcc cgattgcggt
     1561 gcttggcctc gtggtcaatg ttgcgagcgc gtggttattg tccggcggac atcatcacgg
     1621 tcacagccat ggtcactctc atggtcatga ccatgatcat catggcgatg agcaccggcg
     1681 catcgagttg cacaccggcg ccgtcgaaat cgaggttttt gaagacaacg tgccgccgcg
     1741 ttttcgcgtg agggcagaca cgggtgtgct gctggcggcc tccgatgtca ccatcgagac
     1801 catcaggccg gatggcggtc gccagctttt cgcgttcgaa gaccgcggcg gatatctgga
     1861 atcgcgcgac gagattccgg aaccgcatgc cttccttgcc aaatttcgcc tcgtgcaggg
     1921 cggccgacac cacgaccacg aactggaatt cgaagagcac gatcacgagg ctgcgcatgg
     1981 cgcgcatcac cgcgacaaca atatgcgggc cgccgtcatt catgtgatgg ctgatgccgc
     2041 ggtatcggtc ttggtgattg tgggattgct gttggcgcgc gcattcggct ggctctggat
     2101 ggatccgctt gccggattca tcggcgcgct ggttatcgcc aactggtcgg tggggttgct
     2161 gcgcgatacc ggcggcatcc tgctcgatcg cacgcctgat ccgcgcgtgg cagagaaagt
     2221 tcgcatggtg atcgaaagcg agggggatca agtcaccgac ctgcatctct ggcgacttgg
     2281 gcccggccac ctcggcgtca tcgtctcggt cgcgacatca ggggcacgcg aggcggcgca
     2341 ttaccggcag cgattggcca aattcgccga cttgtcccat gtcaccgtgg aggtccaaca
     2401 ctgtcattcg acaaatggct gacccgaccc gcccggctgc cccggggcgg gtcatctttt
     2461 ttaattcctg atgattcatt ccgcgtgctg tgttgcacgc acgtcactca acagcgccgt
     2521 ctgccatctt cttgctattg cgacctactc gcaagtactt gccggacagt tggaaccgtg
     2581 tcattattgt tgcgaaaaag tgtcagtagc attcagcggc aaaaatgcgg gtttgggtca
     2641 attgggcatg ttatctcgga acaggcatcg taatgatgct tgccggggtc ggccccacaa
     2701 attccgcgga ttattcggta agccgaccaa ccaagagctt ggctccggta ccttttttga
     2761 attggaccgg attttatgtc ggcggacacc tgggctacgg ccgtggacat gcaagcggca
     2821 gcttgacgga cgcggttccg acaatttcga gcaattcctt cgggagcctc tttggcggct
     2881 tgcagcttgg ctacaaccat ctcctgacgt caggctttct tgtgggtgtt gaaagcgacg
     2941 tttcgtttgc aaacttcctg ggcgctgacg atatcgtgcg gtcaaggttc acagcgcagg
     3001 gaaaccttgc ggaaaaaatc gactatattg caaggttgcg cggccgcttc ggctacgcct
     3061 ccggcgattg gctgttctac ggaactggcg gctttgcctg gtcgcaagcc cgtttcatcg
     3121 agactcccgg tctaatcgcc aatgaagaca aggtccttcg gacacgccca ggctgggttt
     3181 tgggcgccgg tgcagaagtc gccattgccc cggactggag cgccaggctg gagtacctgt
     3241 acgataattt tggaagggtc gatgccacct tgccatcggg gaggcgctcg gaatccacct
     3301 tcgatatgca tacgctgcga ctggggttga actggcactt agggcgagcg gcatccgaat
     3361 ccccctcgat cgagaaccgc gcttacccgg tcattgcacc cgacaattgg aacgtgcacg
     3421 gacaatatac attcgtcgga cagggctatc cgagcttcca ctccccttat gaaggtccga
     3481 acagcctggc cgggtccaag caagctcaaa acaccatgag cgcgacggca tttgttggtt
     3541 tgcgtctttg ggagggcgct gaattttaca tcaatccgga attgatgcag ggctttggcc
     3601 tcagcaacgt gcatggcttg gccggattct caaacggaga ggcccaaaag tcgagtttcg
     3661 cattgccgcg tatgaatgtt gcgcgcattt tcatacgaca gacttttggg cttggcggcg
     3721 agcaagagac gatcagcgac ggtcccaacc aactcgccgg caagcaggac atctcccgca
     3781 ttacggtgac cgcaggaaaa ttcgccgtta ccgatgcctt cgacggaaat gtttacgcca
     3841 acgatccgcg aacgactttt ctcaactgga atatttacgg cggcggttcc tatgactgga
     3901 ccatggacaa gttaagctgg acatggggcg ctttcgtgga tttcaatcag aaagactggg
     3961 cgttccgcac cggctatttt ctgctgccag tggtgtccaa cagcaacaat ttcgacacgc
     4021 acattccgga ccgcggccag tatacggctg aactcgaact gcgatactcg ctgttttcgc
     4081 aacctggcaa attgcgcttc ttcggctggc tcaatcacgg cacgatgggc ggttactccg
     4141 acgcgcttgc actacctcta actagcccag attatcccga cataacgctg acgcgccgca
     4201 tcagaaccaa ctacggtcta gtcgccaata tcgagcaggc gattaccccc gacctcggca
     4261 tgttctcccg cgcaacgtgg agtccgggtc gcaccgaaat tctcggatgg actgattgca
     4321 gcgaaagcct ttccttcggc accgtgctga acgggacaag ttggggaagg cccgacgata
     4381 aaattggcgt cgcgggtgtc gtcgaaggcc tgtcggccga atcacgcgct tatttcgctg
     4441 ctggcggtct cgggatatta attggcgacg gacaacttaa ctaccggagg gaacaaatcc
     4501 tcgaagcgta ttacgcctac agcctcaaca aatgggcgac gcttaccttc gactaccagt
     4561 tcatcggcaa tcccggatac aatgccgacc gcgggcccgt ttcggtattt tctggcagat
     4621 ttcacgcgga gttctgacca aggacgacgc acacgctttt cgcaatcgtc cggcgttact
     4681 ccgaccgaat atcgggagtg agtgtcgcca gcaccttgtc gatgcgacgt ccgtcgagat
     4741 caacgatttc gaagcgccaa ccatcccatg tgtgcagtcg agtcccgcag aaaacgacat
     4801 cccgcgcatc gacgggattc tggaatgacg ggcgcatcgt caaccccgcg tcgcacccga
     4861 cccgcccggt tgtccccggg ggcgggtcat ctttttagat ccgaatgatt cattccgtgt
     4921 gttgtgttgc acgcacgtca ctcaacgatg ccattcaaca gcatcttttg tgttcattct
     4981 tgcaattgca aatgagttgc aatagggctg agacaatggc attcggcaaa aaatacaggg
     5041 aatgaactgc gcctccgttt gctctggctt cttgaccgcg ggaatcggct aatccgaagg
     5101 gcggggccga cagcttcgtt tgatggatgg cagcgcgccg gcatgatcgc agggacgacg
     5161 aaagagaccg ccggaatgcc cgcgctggaa atatccgcac catgatccgc agcagccgat
     5221 cacgggggtg cgtattcgcg ggtttgggct tgagcctgct cgtcgtcgac ggctccgcga
     5281 ttgcagccga catgcctgtt aaggctccct acattcggtc ggtgttcgat tggaccgggc
     5341 tctatgtcgg cgcccatgct ggcttcggcc gtggctcctc aaccgccgtg cttgcggatc
     5401 ccgcgaccag cacaaccagc aactccttca gcggcatgat cggcggcgtg caagcgggct
     5461 acaattacca actgccatcg gggatgctgc tcggggtgga agccgatttc tcctttccga
     5521 attatcttac ctcgaactcg gtcgtctcct cattggcgac ggcccggtcc gacgtcaccg
     5581 agcaatggga ctatgtgggg accgcgcgcg gccgcgtcgg ttatgtcgcc ggtccgtggc
     5641 tggtctatgc caccggcggg ctcgcatggg ccggcgagcg ttttctcaac acgcccgcca
     5701 tcggcagcga tgaaaagacg ctgcacaccc ggctcggctg ggccgccggc ggcggggtgg
     5761 aatatgcctt cgcgccgcac tggagtttgc ggctggaata tctctacagc cagttcggaa
     5821 acgccgacat caagtttcca tcggccacgc agtacacctc cggcctggat ttccagtcgc
     5881 tgcggattgg cctcaaccgc aagatcgact ggccgggatc gggcaacttc tcaccggcat
     5941 cgctgaccga tcccgaatcc gatcgctggg aaatccacgg ccaatcgacc tatttgccgc
     6001 aaggctatcc gggattccgc gcgccctatt cgggtccaaa cagcctgacg ccggcgccgc
     6061 aagtccaggc aacctggagc aacagcctgt tcctgaacgc ccggctgtgg gagggcggcg
     6121 aggtctacta caaccccgaa ctgctgcagg gatttgggtt gaatgacacg gtcggagctg
     6181 ccggctttcc cagcggcgaa tcgcaaaagt ccaacttccc ctacccgcat tataacacct
     6241 cacgcctgtt cctgcgccag acgtttggct tcggcggcga gcaggaagaa ctcgccagcg
     6301 gaccaggcca actcgccggc aaggcggacg tctcgcggct gacactccag gtcggcaaat
     6361 tcgcggtcac cgatgtcttc gatggcaacg cctacgccaa ggacacccgc aaggatttca
     6421 tgaactggtc gatgtgggcg cccggagcct tcgactatgg cgcggacaag ctcgggctga
     6481 cctatggcgc caccgcggag ctcaaccaga agcaatgggc gttacgtgcc ggctatttcc
     6541 tgatggacgc ggtatcgaat tcaaacagct tcgacaccaa tctttttcag cgcggcgagt
     6601 atgtggttga gctcgaaacg cgctattcgc tgttcaacca gccgggaaag ctgcggacca
     6661 tcgcgtggct caacagtgcc ttttccggaa gctatcgcga gacactggat aatccggcat
     6721 taaatctcga cattgcacaa acccgaattg gccgcatcaa atatggctat gtcatcaatc
     6781 tcgaacaggc cttgaccgac gaggtcggct tgttcggccg ctggagctgg aacgacggca
     6841 agaccgagat catggcgttc accgatatcg atgccagcct ctcgctcggc gtctccatca
     6901 agggcacgcg atggggccga cccgacgacg tgatcgggat cggcggcgcc atcaacgcgc
     6961 tttcccggga tcatcgcgac ttcatcgcgg ccggcgggct aggccccctg atcggcgatg
     7021 gccagctcaa ctatcagccc gagcgaatcc tcgaaagcta ttatgcgtac gcgctcaaca
     7081 agcagctcac gctgaccggc gattatcagc tgatcaccaa ccccgcctac aatgcggacc
     7141 gcggtccggt ccatgtcttt tcaggccgcc tgcacggcga gttctaaatc ttcggcgagc
     7201 gaacgaggca agcgtaagcg ggcgcttcgc tccccaagcg atcccctatc tcggcttgcc
     7261 tgcgaacaat ccatcaaacc gaaatgagta tcctattccg acaatatagc tcggggcatt
     7321 atggttgagt ccgaaagcga catggaaatc gacctgttgc gtcgggctca gatgatacaa
     7381 tccgcccgaa ttcagcagct ggctcggacc agcattgtcg gggtaatcgc cgacatattc
     7441 cacaaacagg ctggccttct cggtcacctt tttttcgatc gcaaaggtgg cttccgtcat
     7501 gcgtttggtc gttacgtccg agggacggaa gaactcggtc agcatgccgc tcacacccca
     7561 actttcattc agttcccacg accatggcaa ttgcaggtaa ggctgcgcgc cgcggcccgc
     7621 tatgtcgacc gcaccggtcg gaagcgccac gccgaagacc acggacaaat cgaccttccc
     7681 cggtatcgga ctgacttgcc actttatcgc cggagcaaca tccgaaaacc ccgagcttcc
     7741 cggtctcccc gtgtttgcaa agtacgtcgg cagatccaac aatagctcca ggcaaggcgc
     7801 tactcccaag cgccatcggg tgtttgtgcc atcgatcgct cgaccgccgt cccgagcgct
     7861 gacattcacg ccgttctcac tctgaagact gcccacgggc acgacgaggc tggagttcgt
     7921 gacatccggc cggtccgttg cgatttggtc cgtggcggtc ggacaaccct ccgcctgtgc
     7981 gtcgaccgcg ccgagaaccg caaacgccga cagcaaacag atctggagag ttcgtctgtg
     8041 cacgagaacc ttgatcgaag gacaaccggc tggaggcatt ggcggaagac aaccgaacta
     8101 gttgcgccgg cttacttcat ctccgtatct tcctcgcgta acccagcatg gacccctatc
     8161 agccatcgac ttgatctccg cagatgaact cttattccca cggctgcccc taaattatca
     8221 tctcatcaca aattccgcct ataacgccga tcgcggtccg gtccctaccc ttccggccgt
     8281 tcataatccc atgtccaggt agagcggctc catggcaagc caacaactgc agcagccgac
     8341 cctcggacag gccctccagc ctaatatctg ggacgtcgtg gcgctgattc tcgtggtcgg
     8401 cgccatggtg ctgatcgtct atggcggcga gcagaccgcc cttcccttgt cggcgctcga
     8461 tgtcacgccg gtatctctcg atccggccaa tctcccggtc tacgcgctgc gcacgaccat
     8521 gcgaatgctg ctggcgatcg tttgctcgat catcttcacc ttcatctatg cagcacttgc
     8581 ggccaagagc cggcgtgccg agatggtgct gatcccgctg ctcgacatcc tgcagtcggt
     8641 gcccattctg ggctttctca ccttcaccgt cgttttcttc ctgaatctct tccccggccg
     8701 ggtgttcggc gccgagctcg cctgcgtgtt cgcgatcttc accagccagg cctggaacat
     8761 gacgttcagc atgtatcagt ccatgcgcaa cgtcccgaag gatctggaag aggcatcgca
     8821 aagctttcat ctcggcggat ggcagcgttt ctggcggctc gacgtgccct tcgccatgcc
     8881 gggcctgatc tggaatacga tgatgtcgat gtccggcggc tggttcttcg tggtggcgtc
     8941 ggaggcgatc acggtcggca acaccacggt cacactgccg ggcatcggct cctacgtggc
     9001 attggccatc aaggaacaaa atctccccgc gatcggctat gccattctgg cggtgtttct
     9061 ggtcatcctc gcgtacgacc agttgctgtt tcgccccgtc gtggcctggg ccgacaaatt
     9121 ccggtttgag cagaccgcct cggcgagcgc gccgacatcc tggatgctcg atctgtttcg
     9181 gcgtacacgg gccttgcgcg caatgaccta tccgtttgca gccctcaaca aagccgtctc
     9241 caacctgcac atcgcgctgc caagccaatt gaaaatggca acgagagcaa gagcccagcc
     9301 atctcgcgtg gtcgatgccg tctggcttgc aacgatcatc gcaggcaccg gctacgccgc
     9361 ctggaggatc tatacatatc tctcggcgac gttgagcccg tcggacgtct ccagcgccgt
     9421 cggatacgga tttattacgc tggcgcgcgt catcgtgctg atcgcgctgg cgacgctgat
     9481 atgggtgcct gtcggggtct ggatcggact aagacccaaa cttgccgagc ggatccagcc
     9541 gctcgcgcag tttctggccg cgttcccggc gaacctcgcg tttccggtgt tcgtggtgat
     9601 catcgtccgt tacggcctga acgcgaatgt ctggctcagc ccgctgatga tcctgggaac
     9661 ccaatggtac atcctgttca atgtcatcgc gggcgccagc gcgttcccga ccgaccttcg
     9721 cgaggctgcc ggaagcttcc atctgaaggg ctggcgctgg tggataaaag tcatcctgcc
     9781 cggcattttc ccgtattaca tcaccggcgc catcaccgcc tcgggcggat catggaatgc
     9841 ctcgatcgtc gcggaagtgg cgagctgggg cgacacccat ctgacagcca caggtcttgg
     9901 cgcctatatc gcgaccgcga cggaagccgg cgattttccc cgggtcgttc tcggcatcgc
     9961 catcatgtgt attctggtga cgctgttcaa ccggctgctc tggaggccgc tctatgcctt
    10021 cggagagcgc cgtcttcgcc tcggctgatc aggaggaact catgctcgat catagcaatc
    10081 agaccaacct tctcgatatc cgtggcgtct gccggtcgtt tccgaagggc agcggtgagg
    10141 acctgctggt cctcgaaaag gtcgatctga cgattcattc cggcgaaatc gtcggcctgc
    10201 tcggccgttc cggatcaggc aagtcgacgc tgctgcgcat catcgccggg cttatcacgc
    10261 catcctcggg cgatgccaaa tgccgtggcg agatcatcat gggcccgccg aacggcgtct
    10321 ccatggtgtt tcagtccttc gcgctgtttc cgtggctgac cgtcctgcag aatgtcgaat
    10381 tggggctgga agcactggga atcgacagcg ccgtgcgccg cacgcgggcg ctcgcggcga
    10441 tcgacctgat cgggctcgac gggttcgagt ccgcataccc gaaggaactc tccggcggga
    10501 tgcgtcagcg cgtcgggttt gcgcgggcgc tggttgtgca tccggatctt ctgttgatgg
    10561 acgagccgtt ttccgcactc gacgtgttga ccgccgagac actccgtacc gacctgatcg
    10621 atctgtggat cgaaggccgg ctgccgatca aatccgtgct gatggtgacg cacaacatcg
    10681 aggaagcggt gctgatgtgt gatcgtattc tggtgttctc gtccaatccg ggacgggtag
    10741 ctgccgaaat caaggtcgac ctgccgcatc cgcgcaaccg tctcgatccg acattccggc
    10801 aactcgtcga cagcatctat gcccgcatga cgcagcgcgc ggaggttaaa acgccggcgg
    10861 tcgagggcat tcatggcacc ggcgtcggca tgatcctgaa tcatgtttcg tcgaacgtcc
    10921 tttcaggtct gatcgagacg ctggcggggc caccgtatag tgggcacgcc gatttgccgg
    10981 ttctcgccgg cagcctgcaa ctcgaagcgg acgagatatt tcacctcggc gaatcgcttc
    11041 agttgttgag gtttgcgcaa ttgagcgaag gcgatctcat gctgacggat gccggcaagc
    11101 gctttgctca cctcgagacc gatgcccgca agaagctgtt cgccgaacac ctcatcaatt
    11161 atgtgccggt gatggacctg atccgccgtg tactcgacga gcggccctcg cataccgccc
    11221 ccgtcgcgcg cttccgcaac gagcttgagg attatatggc ggaagatcag gccgatgaaa
    11281 cgctaaggac aatcgtgtcc tgggctcgct atgcggaact gttcgcatat gacgagcagt
    11341 cggagctgtt cagcctcgaa aacccgcatt agcgccaccg tcgtcccggc gaacgctcag
    11401 tacttcacac cgccgccgca aacgtctcgg ggcgcgccat ggcccaggct tcgcgatagc
    11461 gcggatcgct ggtcccgtcg atcagttcac ccggacgcag cgacggatag agctgcgcaa
    11521 atgaccttac ctcggtggtg gagcttcgct gcgagaaatg aatcgcccgg atctgctgcg
    11581 gatgttcgag ccccgcggcg gcgatgagtt cggccagcgc gtgcagcgtt gcgcgatggt
    11641 agttgtagac ccgctcgatc ttgtgcggca ccaccagcgc acgagcgcgt gatggatcct
    11701 gcgtcgtcac cccggtcgga catcgatcgg tatggcagct cagcgactgg atgcagccca
    11761 gcgagaacat gaaaccgcgc gccgaattgc accagtcggc gccgatcgcc atcgcgcgcg
    11821 ccatgtcgaa cgcggtcgcg atctttccgg cggcgccgat gcggatgcga tcccgggcat
    11881 tgatgccgat cagcgcgtta tggacgaaat tgacgccttc gcgcatcggc atgcccaaat
    11941 ggtccatgaa ttccagcggg gcggcgccgg tgccgccttc attgccatcg accacgatga
    12001 aatccggata gattccgctc tggaccatgg ccttgcagat cgccagaaat tcccagggat
    12061 ggccgatgca aagcttgaat ccggcgggct tgccgccgga caaacgccgc atctcggcga
    12121 tgaaggccat catttccagc ggcgtcgaaa acgcccgatg cgtggccggc gagatgcagt
    12181 cctcgtccat ggcgacgcc
//
LOCUS       DASWGM010000125        18106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_562018,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000125 DASWGM010000000
VERSION     DASWGM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18106
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562018"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VGA15_03825"
     CDS             <1..1148
                     /locus_tag="VGA15_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9586855.1"
                     /translation="AGIDTLVLEKHADFFRDFRGDTVHPSTLEVMDELGLIEGFLKLP
                     HQRLQKLDGKFGGTSIRVGDLSRLKVKYPFIAFMPQWDFLNFLRESGKRFSSLKVMMN
                     ADATDLRWSGDGVVGVLADTPDGPLEIRADLTIGCDGRHSIVRQCANLEVEEIGAPMD
                     VLWFRAGKGANQAESVFARVETGKMMVTFDRGEYWQCAYVIAKGQYDAVKARGLDAFR
                     DDIVGMAPILKSGISEVKTWDDVKLLTVAINRLKRWTRPGLLCIGDAAHAMSPVGGVG
                     VNLAVQDAVATANLLAAKLAEGCPDENELDAVRRRREFPVRMTQTMQVVAQNNIISVA
                     LNSAGRELKVPLFARLINAVPWLQGITARFVALGVRPEHVRSPALPG"
     gene            1512..2225
                     /locus_tag="VGA15_03830"
     CDS             1512..2225
                     /locus_tag="VGA15_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9586856.1"
                     /translation="MAADMAVKARPVAPPVVIYNWSGCYIGGNVGGGWKRNTLSSTTT
                     FLGTPVAFTDLGSDNGSSVIGGAQIGCDYQFAGNWVAGIQGMFDFGNIDSSHQIPGFP
                     GFFETSRVKDIFSVTGRVGYLFTPQLLGYVKGGGAWARTNLSILGPANATVEFASNNR
                     SGWTVGGGLEYMFAPGWSVFGEYNFMDFGHRNLAFAASPGFVFTDTVSSHLTVQEALV
                     GVNYKFNWGWGGAPVVARY"
     gene            2638..3456
                     /locus_tag="VGA15_03835"
     CDS             2638..3456
                     /locus_tag="VGA15_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9586857.1"
                     /translation="MRRYFGWTLASVISLGLFGGLGGASAADMAVKARPAPVALAPGW
                     TGWYVGLNAGWVGTNKGTLTNTGTDTGTGGYGTGLGNGAYPLAVGLSNQGFLGGAQFG
                     YNYQSGMWLVGFEADIDGVSAKKTTVFLGGPPAFTPNTETYTRELDWLATVRGRVGVV
                     ASPNLLLFGTGGLAVGQTKIGSKVTCPLAGPPCESEPTNNLTTTNTSAGWTAGAGFEW
                     MFAPQWSLKGEYLYVDLGRHSNTLTYTYGPFTSTLTSTVRDTYNIGRAGVNYHF"
     gene            complement(3677..3805)
                     /locus_tag="VGA15_03840"
     CDS             complement(3677..3805)
                     /locus_tag="VGA15_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586858.1"
                     /translation="MANASITAQIIVMPSRTSIFLLQAFFTTLSINFGRQLKTIRS"
     gene            3974..4762
                     /locus_tag="VGA15_03845"
     CDS             3974..4762
                     /locus_tag="VGA15_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9586859.1"
                     /translation="MRKMSNSKSIAALAFTAAIGVGAASAADLGARSYYTKAPAIADA
                     LYNWGGFYVGGHVGGSSTNERWTNVANTTAFGDLSPGQGFGQRGTGVFGGGQIGYNWQ
                     ASNYVFGLEGTVSAMNNRGSLANTVFGAGDDVFSWRTNWMATVTGRIGHAVENNLFYA
                     KGGYAGINNRLAVSDVVPPVVGSGSDSHWHNGWTIGAGWEYGMTRNWTVGLEYDYSAF
                     ETKSYQLAGAAAGVYTFDAKPRDIQSAVVRLNYRFDGPGFSPYR"
     gene            complement(4839..5609)
                     /locus_tag="VGA15_03850"
     CDS             complement(4839..5609)
                     /locus_tag="VGA15_03850"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9586860.1"
                     /translation="MTSKLFDLSGKVAIVTGGNGGIGLGMARGLARAGANVVIVGRNE
                     AKSDAAVAELSQHGVEAISVAADVTDKAAVTAMVERTLRELGRIDILVNNAGINIRKA
                     PHALELEEWDSVIKTNLTSAFLCSQAVYPAMKAAGGGKIINIGSMMSIFGASFAPAYA
                     ASKGGIVQFTRSSAVAWAADNIQANAVLPGWIDTDLTKRARQEIDGLHDKVLARTPAA
                     RWGGIDDFAGIAVFLASSASDFVTGTAIPVDGGFSVMG"
     gene            complement(5813..5998)
                     /locus_tag="VGA15_03855"
     CDS             complement(5813..5998)
                     /locus_tag="VGA15_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586861.1"
                     /translation="MRLLLLILGILALLIGLLWIGQGTGYVAWPQSSFMISQIQWAYY
                     GAALAVVGLILIWQGKR"
     gene            complement(6178..6960)
                     /locus_tag="VGA15_03860"
     CDS             complement(6178..6960)
                     /locus_tag="VGA15_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX9586862.1"
                     /translation="MKKLVLALSAVAAFTGSALAADLPARTYSKAPMMAPVYSWTGFY
                     IFGGAGGGLWDADSNVLTTTAPITALTRDQRVGGDGWFGTVGAGYDWQFNHSWVAGIF
                     ADGQFGSLRGSISDPNIGFEGREKLKTSYAAGARIGYLVAPNVLSYVNAGYSGSQWSG
                     SSMTALFAGVPGVVTTPSFHRDGWFVGGGVENNLDIFGISAPGWFMKTEYRGAYYNRI
                     TLPETFAGAPLGSAVTFKPFVQTISTSLVYRFNWGGPVVAKY"
     gene            complement(7207..7438)
                     /locus_tag="VGA15_03865"
                     /pseudo
     CDS             complement(7207..7438)
                     /locus_tag="VGA15_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087127.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
     gene            7487..7774
                     /locus_tag="VGA15_03870"
     CDS             7487..7774
                     /locus_tag="VGA15_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9586863.1"
                     /translation="MIRSWRNAAARKFWEGHPSNQFRGLDVDSAIDLLLALNVAKSLQ
                     DLSPLRSVGLHKLKGARKKQWAMTVNDRWRICFEFRNGDAFDVEIVDYHKG"
     gene            7778..8065
                     /locus_tag="VGA15_03875"
     CDS             7778..8065
                     /locus_tag="VGA15_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX9586864.1"
                     /translation="MAPAIHPGRLLKRELGSRKLSANRLALDLGVPSGRITDILNGRR
                     SISADTAVRLGRYFGNHAQFWLDLQSQYDIAVIEREKGADISRRVRPADAA"
     gene            complement(8089..10146)
                     /gene="parE"
                     /locus_tag="VGA15_03880"
     CDS             complement(8089..10146)
                     /gene="parE"
                     /locus_tag="VGA15_03880"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX9586865.1"
                     /translation="MDKPLKNNAKAKSANDLFGASEPKGRAPLKAAPRAATAEAGYTA
                     ADIEVLEGLEPVRRRPGMYIGGTDEKALHHLFAEVIDNSMDEALAGHASFIEVELSAD
                     GFLTVTDNGRGIPVDPHPKFPKKSALEVIMCTLHSGGKFDSKVYETSGGLHGVGVSVV
                     NALSSRLEVEVARSQKLYRMTFERGHPKGKLEDLGKINNRRGTRIKFKPDTDIFGAKA
                     TFKPQRLFKMARSKAYLFGGVEIRWRCDPALLKGIEDVPAEDTFHFVGGLKDFLAAAV
                     ASDTLVHPDIFSGKSGRVGAHGACEWAVAWTADADGFMSSYCNTVPTQDGGTHESGMR
                     SALLRGLKDHAERVGQGKRAASIVSEDVMVGAAVMLSVFVREPEFQGQTKDRLATAEA
                     QRIVEQAIKDPFDHWLSANPLQASKLLEFVVERADERLRRRAEKEISRKTAVKKLRLP
                     GKLADCTNTAAEGSELFIVEGDSAGGSAKQARDRKTQAILPLRGKILNVASATKDKLT
                     ANAQLADLMQAIGCGTGAHYRQEDLRYARIIIMTDADVDGAHIASLLITFFYRQMPRL
                     IDEGHLYLAVPPLYRLTHGSKTIYARDEAHKDALLKSEFNANAKVDVGRFKGLGEMMP
                     AQLKETTMDPAKRSMLRVVLLADDRAGTADSVERLMGTKAEARFAFISDKAEFASEDL
                     LDV"
     gene            complement(10291..10440)
                     /locus_tag="VGA15_03885"
     CDS             complement(10291..10440)
                     /locus_tag="VGA15_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586866.1"
                     /translation="MIGAILFPAGVALVLPKGDSFIAAPIVGSLIVVMAMALFAVIVF
                     RTSRV"
     gene            complement(10437..10634)
                     /locus_tag="VGA15_03890"
     CDS             complement(10437..10634)
                     /locus_tag="VGA15_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586867.1"
                     /translation="MMCLSVLVGMLAGIAMGIQQNFALGPAHAHLNLVGGVLLFLFGC
                     TTGWFRLPGPRPLPRCGAGST"
     gene            10818..11348
                     /locus_tag="VGA15_03895"
     CDS             10818..11348
                     /locus_tag="VGA15_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9586868.1"
                     /translation="MEDFAHALADFVRDHRVWAAPIVLLLAFGESLAFISLLIPAWGA
                     LVAIGALIGASGISFWPVWIAGGIGAAVGDWVSYWFGFKYKEQVARMWPLSRYPELLP
                     RGEAFVKNWGVPSIFIGRFFGPLRASVPLAAGIFEMPYWSFQFANFVSALVWSAALLL
                     FGDVIAQILEWLWRVV"
     gene            11524..12513
                     /locus_tag="VGA15_03900"
     CDS             11524..12513
                     /locus_tag="VGA15_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9586869.1"
                     /translation="MELTRRHALAGAAAIAATQLLPSVPVRAAAPVADKQAPSFYRYK
                     VGDIQVTVVSDGKNVFKLEDSFITNAKKDEVNAALEKAFLPRDIMSIYFAPLVINTGG
                     KLVVLDTGNGAIAKANSKGANGLFADNMAAAGFDANAVDMVVISHFHGDHVNGLLTAD
                     GTPTYPNAEVLVPATEWKFWMDDGEMSRASGPRMTGLFKNNRNIFEAALKKKVTPYEW
                     DKEVAPGMLAVETAGHTPGHTSYVLSSGSGKVFIQSDVTNNPNPFATNPGWHAFFDQD
                     GDMAEKTRRRVYDMVAAEKLQVQGFHYPFPGLGNVEKDGSGYRVIPAPWNPTI"
     gene            12932..13597
                     /locus_tag="VGA15_03905"
     CDS             12932..13597
                     /locus_tag="VGA15_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX9586870.1"
                     /translation="MFTRPFFEPDRAASLAFADARGFGTVCACDGGKPVASSLPFYLS
                     YGDDGTPHALFHVARQNALATLADGKSSWLIAVNGADAYVSPDWYASPDQVPTWLYQA
                     VHLTGTVRRLSDHELGPHLDALSAKFESWLMPKPPWMSSKMTAARLDAMKKAIVGLVM
                     TVEEIEGSFKLNQHKSDVDHAAIADALALQADAGAQQLARTMRTMRPQAFTDETVQLE
                     RSV"
     gene            13597..14646
                     /gene="argC"
                     /locus_tag="VGA15_03910"
     CDS             13597..14646
                     /gene="argC"
                     /locus_tag="VGA15_03910"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX9586871.1"
                     /translation="MSTKKKIGILGASGYTGADAVRLLARHPDAEIVALTANTHAGKA
                     MDEVFPHFFMLDLPRLTEWEKVDWTKLDAVFCGLPHGTTQEIIAAVLAANPAIKILDM
                     SADFRLRDVSEYAQWYGHEHRAPKLQGEAVYGLTEFYRENITAARLVACPGCYPTAAL
                     LALVPIAKAKLIDVDDIVIDAKSGVTGAGRGLRQNTLFSEAGEGISPYSIASHRHSPE
                     IEQEIGVAAGKKVTANFTPHLIPMSRGELCTSYVKLNGACADDLRAALAEAYKDEPFV
                     HVAKKGVLPQTQNVRGSNYVQIGVFADRIKNRAIIISVLDNLVKGSAGQAIQNMNLML
                     GFPETTGLEQIALFP"
     gene            complement(14651..16006)
                     /locus_tag="VGA15_03915"
     CDS             complement(14651..16006)
                     /locus_tag="VGA15_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9586872.1"
                     /translation="MILRMTFAARIALIVVVGLGMAWISAIALYYLARAHTAEDNTLP
                     VPGHIAAVVELIERTPGPERALVLRAASSETFSARIDDAATIGATAPRILPRLNARAI
                     EQSLQPLGGRPVSVNFSDAGERWRLFSRLVPTTIDVRVGLATGETLIIEIGNRILVGP
                     LGLPVGFGAGLFGSLIALLALFVMHRETKPLARLAEAVDRVDLSLDPVALPEAKRSAP
                     EIRALIDAFNRLQNRLGELLRSRMVMLGGISHDVRTYATRLRLRAEKISDATERDRAI
                     ADINDMIVLLDDALLASRAGAGELAEEMVEIDDIIGAEVADRRSGGARIDYQRGQWEQ
                     VPIVLGDRLALRRVVANLADNALKYGGAAHLRSVIVGNAIDLTVDDEGLGIPPEQRQA
                     MLEPFSRLETSRNRGTGGAGLGLAIARGLVEAHRGSLSIGQAPSGGARISVRLPLFCS
                     N"
     gene            complement(16003..16722)
                     /locus_tag="VGA15_03920"
     CDS             complement(16003..16722)
                     /locus_tag="VGA15_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9586873.1"
                     /translation="MTTPKPTILIVEDDPEIRRLVRDFLLHEGYDVETAEDGKAMDVV
                     LQRVRPDLIILDLMLPGEDGLAICRRLRTGDPIPILMLTAKSDEIDRVVGLEMGADDY
                     LAKPFSSRELLARARAIMRRTQPTAPQPAQNRRYRFDAFVIDIDARGLSAGENNAVNL
                     TSGEFELLVCFVQRPRRVLTRDQILDWTRGRSADPFDRTVDMLISRLRKKLDGASPGS
                     NLINTVRNGGYLFTAHVSPLT"
     gene            16913..17215
                     /locus_tag="VGA15_03925"
     CDS             16913..17215
                     /locus_tag="VGA15_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586874.1"
                     /translation="MKKLFAIAAIVGALSAAAIVDANAGSRSGSISGPRGTTTFAGSR
                     SCAGGACSSQGSVTGPYGGTISHQGSSSCSGGTCSGGGTVTGPRGGSVTYNGTVTR"
     gene            17281..>18106
                     /locus_tag="VGA15_03930"
     CDS             17281..>18106
                     /locus_tag="VGA15_03930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX9586875.1"
                     /translation="MNQLAGSLAASSRLMLLATALGFMGLEYFLGRLAHHDTHDPFES
                     AASLGVAAGQSVVRAVEATLLAIPFAFVYQHRLFDFSQTTPLALAALFVAMEFAYYWQ
                     HRASHRIHWMWATHAVHHSATKLNFTAAVRLGWTGNISGNFLFFLPLVWFGFHPFAVI
                     GMLGVNLFYQFFIHTELVPRLGPLEWILNTPAHHRVHHASNPACLDKNYGGILIVFDR
                     MFGSFAEAPPHEALRYGLVHGTSSYNPVRIALGEWRNMFRDFARATTLRARVKTLLG"
BASE COUNT         3422 a         5416 c         5711 g         3557 t
ORIGIN      
        1 gcgccggcat cgacacgctg gtgctggaga aacacgccga cttcttccgg gattttcgcg
       61 gcgataccgt gcatccctcg acgcttgagg tgatggacga actcggcctg atcgagggat
      121 tcctgaaact tccgcaccag cgactgcaaa aactggacgg gaaattcggc ggcacctcga
      181 ttcgagtcgg ggatctcagt cgtctcaagg tgaaataccc cttcatcgcc ttcatgccgc
      241 aatgggattt tctcaatttc ctgcgcgaga gcggcaagcg gttttcctcg ctcaaggtga
      301 tgatgaatgc cgatgccacc gatctgcggt ggtcgggcga tggggtggtg ggcgtcttgg
      361 cagatacgcc ggatggaccg ctcgagatcc gcgcggatct gactatcggc tgcgatggcc
      421 ggcattcgat cgtgcggcag tgcgccaatc ttgaggtcga agagatcggt gcgccgatgg
      481 acgtgctctg gttccgggca ggcaaaggcg ccaaccaagc cgaaagcgta ttcgcgcgcg
      541 tcgagaccgg caagatgatg gtgacgttcg atcgcggcga atactggcaa tgcgcctatg
      601 tgatcgccaa gggccaatat gacgcggtca aggcgcgcgg gctggacgca ttccgcgacg
      661 acattgtcgg catggcgccg atcctgaaat cggggatctc ggaagtgaag acgtgggacg
      721 acgtcaagct gctgacggtg gcgatcaacc ggctgaagcg ctggacccgc ccggggctgc
      781 tctgcatcgg cgacgccgcg cacgcgatgt cgccggtcgg cggcgtcggc gtcaatcttg
      841 cggttcagga tgcggttgcg accgcgaacc tgctggcggc gaagctggca gagggctgtc
      901 ccgacgaaaa cgaactggac gccgtgcgcc ggcgccggga atttccggtg cggatgacgc
      961 agaccatgca ggtcgtcgcc cagaacaaca tcatcagcgt cgcgctcaat tcagccggtc
     1021 gggaactcaa ggttccgttg ttcgcgcgtc tcatcaacgc cgtgccgtgg ttgcagggta
     1081 tcaccgcgcg attcgtcgcg ctcggcgtgc gaccggagca tgtgcgttcg ccggccttgc
     1141 cgggctgacc gtcgatctcc tgatcagcac gcttgatcga atcggtacag ggcggtggca
     1201 tcgcgttggc cgggtgttgt cggcgttgca gtgcgggagc tgtgtcggca taagcgggcg
     1261 gcgcgtggtt cccttaattg tatcgtatta ccgcgaccgc aaccttctgt tgcagagatg
     1321 caacgctggt acgaaaaatg tgcctgtaga gcctcctggt tgcagttccg tgtcgctgaa
     1381 acgcgcaatc gagcctaaca attaatcgca cttagattct tgtattgttg ctggaggaca
     1441 ttatgcgccg acatcttgga tgggctctcg cctcggttat cttggtagct cttagtgggc
     1501 tcggcggcgc gatggccgcc gatatggccg tcaaggcacg cccagtcgct cctccggttg
     1561 tgatctacaa ctggtcgggc tgctacatcg gcggcaatgt cggcggcggc tggaaacgaa
     1621 acacgcttag cagcacaacc accttcctcg gtacgcctgt tgcgtttacg gatttgggtt
     1681 ccgacaacgg cagcagcgtt atcggtggcg cccaaatcgg ttgcgactat caattcgccg
     1741 gcaattgggt agccggcatc caaggcatgt tcgacttcgg caacattgat agctcgcacc
     1801 agatcccggg cttccctggt ttttttgaaa caagtcgggt taaggacatt ttcagcgtta
     1861 ccggccgcgt cggctacctg ttcacgcccc aactgcttgg ctacgtcaag ggtggtggcg
     1921 cctgggcacg caccaatctg agcattctag gccctgctaa tgcgactgtt gagttcgcca
     1981 gcaacaatcg gtccggctgg accgtgggtg gcggccttga gtatatgttc gctcctggct
     2041 ggtcggtatt tggcgaatac aattttatgg acttcggaca ccgcaacctc gcctttgcgg
     2101 cgtcacccgg ttttgtcttc acggataccg tatcatccca cttaaccgtc caggaggcgt
     2161 tagtgggcgt gaactacaag ttcaactggg gttggggtgg cgcacccgtt gttgcgcgat
     2221 actgatctga ctctctcccg aaactgacca aagccccggc ctcggccggg gcttttttgt
     2281 gtgatgggtt tcagagaaga ttgctctttc gtttcgctgc cgagcaaaga aacactgcga
     2341 tgcccgtgga cgatcgggcc gtggggcgga tgggtcggcg gcagcaggcg cgtgtgaacc
     2401 ttacgattct ataatttttt gcaacatagc gggcggcgcg ggcttccctg agcggtatca
     2461 aattagcgtg ccagcatctt tttgttgcag gcgcgcaaca atgccacaga aaccgcacct
     2521 atatgccctt tatggggcat ttctttgtcg cctgagtccc agaggtatca tacgagtggt
     2581 ggagcgcatc ctgcaaatgg gggagcgttt tatctgtact gcgtgctgga ggagaacatg
     2641 cgtcgatatt ttggatggac tcttgcttcc gttatttcgt taggtctttt tggggggctc
     2701 ggcggcgcct cggctgcgga tatggccgtc aaggccaggc cggccccggt cgcgctggcc
     2761 cctggctgga ctggctggta cgtaggtctg aacgccgggt gggtcggtac gaacaaaggc
     2821 accctcacca acacaggtac ggacactggc acgggtggct atggaacggg gctcggtaac
     2881 ggagcttatc ctctagcggt cgggctatct aaccaaggtt tcttgggcgg cgctcagttt
     2941 ggctataatt accagagcgg aatgtggctc gttggtttcg aagccgacat tgacggcgtt
     3001 agcgcgaaga agaccaccgt ttttttaggg gggcctccgg cgtttacgcc aaacaccgag
     3061 acttacactc gtgagctcga ctggctcgcc accgtacggg ggcgcgtcgg cgtggtcgcg
     3121 tcaccaaatc ttctgttatt cgggaccggc ggcttggcgg taggccaaac caaaataggc
     3181 agcaaggtca cgtgcccgct ggcaggcccg ccgtgtgagt cagagccgac caataatcta
     3241 accaccacta acacgtcggc cggctggact gcaggtgcgg ggttcgaatg gatgttcgcg
     3301 ccacaatggt cccttaaggg cgagtacctg tacgtggatc tcggccgtca cagcaacacg
     3361 ctgacgtata catacggacc ttttacgagt acgttgacca gcacagtgcg tgatacctac
     3421 aacatcggca gggctggcgt gaactatcat ttttagcggc ccggtcgtcg cgaagtactg
     3481 atcttcccga aaccattcgt cggatcaaaa gccccggcct cggccggggc ttttttgtgt
     3541 gatgggttca gagcagattg ctgtttcgtt ttcgacgctg ccaaaaccct gcgatgcctg
     3601 cggacgagga cgaacgcgcg agcgcgatgc ggttgcaaaa acttgctgat ccgcgtgctg
     3661 gcgatcgctt tgtttttcac gatcggatcg ttttgagttg gcgaccgaaa ttgatcgaca
     3721 acgtggtgaa gaaagcctga agcagaaaaa tcgacgtcct gctcggcatg acgatgatct
     3781 gcgccgtgat gctggcgttt gccacgaaga attgctgttc atggccgcat tcgtgcgtcg
     3841 cgtgtgtcag ttgcgagcct ccaagagatt ccggcaatcc cgcgtcgctc ggcgcctcct
     3901 aacgaattgg cgaggaaaat aggggcagta tgttttcgag gaaaagatcg tgggaaaaac
     3961 tggggtacaa ataatgcgta aaatgagtaa ctcgaaatct atcgccgcgt tggcattcac
     4021 tgccgccatt ggggttggag ctgcatctgc cgcggacctg ggcgcgcggt cctactatac
     4081 caaggcgcca gccatcgctg atgcacttta caactggggc ggcttctacg tgggaggtca
     4141 tgtcggcggc tcctcgacca acgagagatg gacgaacgtc gcgaatacga ccgcgttcgg
     4201 cgatctcagt ccgggccaag gcttcggcca gcggggaacg ggcgtatttg gcggtggcca
     4261 gataggttac aactggcagg ccagcaacta cgtgttcggt ctggagggca ccgtttccgc
     4321 gatgaacaac cgcggaagcc tagcaaacac ggtgttcggt gccggggatg atgtgtttag
     4381 ctggcgcacc aattggatgg cgactgtcac gggccggatc ggccacgcgg tggagaacaa
     4441 tcttttctat gccaagggtg ggtatgccgg catcaacaac cgcctggcgg tctccgatgt
     4501 ggttcctccg gttgtcggct cgggctctga cagtcactgg cacaatggct ggaccatcgg
     4561 cgcaggctgg gaatacggaa tgacccggaa ctggactgtc ggtctcgaat acgactactc
     4621 ggccttcgaa accaagagct atcagctcgc cggcgccgct gccggggtct acacattcga
     4681 tgcgaagcca cgcgatatcc agtcggccgt tgtccggttg aactacaggt tcgatggccc
     4741 ggggttctcg ccgtatcgat agcgaccaag cctcaagcga taaaaaagcc ccggcgtcga
     4801 ccggggcttt ttcttgatcg cggcgaacgg tgctttgctt atcccatcac cgaaaatccg
     4861 ccatcgacgg ggatcgcggt gcccgtgaca aaatccgacg cgctggatgc gagaaacacc
     4921 gcgatgccgg cgaagtcgtc gatcccaccc catcgcgccg ccggcgtgcg cgccagcacc
     4981 ttgtcgtgca ggccgtcgat ctcctggcgc gcgcgtttgg tcagatcggt atcgatccag
     5041 cccggcagca ccgcgttggc ctggatgttg tcggcggccc aggcgacggc ggaggagcgg
     5101 gtaaattgca cgatgccgcc cttgctcgcc gcataggccg gcgcgaagct cgcaccgaag
     5161 atcgacatca tcgagccgat gttgatgatc ttgccgccgc ccgcagcctt catggccgga
     5221 tagaccgcct gcgagcacag aaacgcgctg gtcaggttgg tcttgatcac gctgtcccat
     5281 tcctcgagtt cgagcgcgtg cggggccttg cggatattga tgccggcgtt gttgacgaga
     5341 atgtcgatcc gaccgagttc gcgcaaggtg cgttcgacca tggccgtgac ggcggccttg
     5401 tcggtgacgt cggcggcgac ggatatcgcc tcgacgccgt gctggctgag ctcggcgacc
     5461 gcagcatcgg acttcgcctc gttgcggccg acgatcacga cgttggcacc ggctctagca
     5521 agcccgcgcg ccatgccaag gccaatgccg ccattgccgc cggtgacgat cgccaccttg
     5581 ccactgagat cgaatagttt tgatgtcatc gctaaatgct cctcgtcatg cccggccata
     5641 gtagtctgcc ttgcgcagac tgcgtaaact tgtctgcgat gccgggcatc cacgtctttc
     5701 ttgccgctgg tgttaaaaga cgtggatggc cgggacatag gcgagcgcaa gcgacgccgt
     5761 ccttcggacg gctgagcccg gccatgacga gtcgattaag cgttgcatcg atttagcgct
     5821 tgccctgcca gatcagaatc aatccgacca cggctagtgc ggcgccgtaa taggcccatt
     5881 ggatttggct gatcatgaag ctcgattgcg gccatgcgac atagccggtg ccttggccga
     5941 tccagagcag gccgatcaga agtgcgagga tgccgaggat caagagcaat agacgcatgg
     6001 atgatccccg tgtttccggc ggcgcgccgc ataacgcagg ccagaatggc gcgcctgctt
     6061 acccgggggc aagagcagcg atgatgtcgc agggtctgcg ccgagccatc catagcccag
     6121 gcacaaaaaa gccccggaca atgccggggc ttttgaattg ctgaagttca gcagaggtca
     6181 atacttcgcg acgaccgggc caccccagtt gaagcggtag accagcgagg tgctgatcgt
     6241 ctgcacaaat ggcttgaagg taacggcact gccgagagga gcgccagcga aggtctccgg
     6301 aagcgtgatg cggttgtagt aggccccgcg atattcggtc ttcatgaacc agcccggagc
     6361 agaaatgccg aaaatgtcga gattgttttc cacaccgccg ccgacgaacc agccgtcacg
     6421 atgaaaagag ggcgtagtga cgacgcccgg gacgccagcg aaaagggccg tcatgctgga
     6481 accggaccac tgcgaaccgg aataaccggc attgacgtag gataagacgt taggcgcaac
     6541 gagatagcca attctcgcac ccgccgcata gctggttttc agcttttccc gtccctcgaa
     6601 accaatgttc ggatcgctga tcgagccacg gagactaccg aattggccgt cagcgaaaat
     6661 gccggcaacc caagaatggt taaactgcca gtcgtaaccg gcacccacgg ttccaaacca
     6721 gccgtcgccg cccacccgct gatcccgggt taaagcagtg ataggagcgg tggttgtcag
     6781 aacgttgcta tcagcgtccc acaatccacc gccggcgccg ccgaagatgt agaagccggt
     6841 ccagctatac accggcgcca tcatgggagc cttgctgtag gtccgggcag gaagatcagc
     6901 cgcgagggcc gatccggtaa atgccgctac cgccgacaaa gcgagcacta atttcttcat
     6961 ttttttcatt tcctcagtaa gtaagcgctc ggacccgacc gcgtgacagt ggtgcgagag
     7021 caccgattcc ctaagctcat ggcgacgact atagccagtt cgtctcaaaa tgctgttgcg
     7081 gcaggggcac acacgcttga aaacactgtc agggaaaagc cttcgaaaaa ggccgataac
     7141 ggcgtcaaat cggctttttt gcaacggtaa tcacacctga ataccgccgc aggggtcgcc
     7201 gcaccctcac acgtccaaaa ggtcctcgct ggcgaattcc gccttgtctg aaatgaaggc
     7261 aaatcgggcc tcggccttgg tccccattag gcgttcgacc gaatcggcgg tgcccgcacg
     7321 gtcgtcggcc aacagcacga cgcggagcat ggtgcgcttg gccgggtcca tggtggtttc
     7381 cttcagctgc gccggcatca tttcgccgag gccccttgaa gcggccgaca tcaaccttca
     7441 tgtcaggtca attgttttgc accgttttgc gtagtatgat tatgccatga tccggtcttg
     7501 gcgaaacgcc gcagcccgca aattctggga gggccatccg tcaaaccaat tccgcggcct
     7561 tgacgtcgat tctgcgatcg atttgctgtt ggcactcaac gtggcgaaga gtcttcaaga
     7621 tctcagtccg ttgcggagcg tcggattgca caaattgaag ggcgcgcgga aaaagcaatg
     7681 ggccatgacg gtcaacgacc gttggcgtat ttgctttgaa ttccgcaatg gcgatgcgtt
     7741 cgacgtcgag atcgtcgact atcacaaggg gtaggacatg gctccagcaa tacatccggg
     7801 acggcttttg aaacgcgaac tcggctcccg caagctcagc gccaaccgtt tggcgctcga
     7861 tctcggggtg ccgtcaggac gcatcaccga cattctcaat ggacggcgct cgatatcagc
     7921 cgatacggcg gtgcggctgg gtcggtattt cggcaaccat gcgcagttct ggctcgattt
     7981 gcaaagccaa tacgatattg ccgtgatcga acgcgagaag ggcgcggaca tcagcaggcg
     8041 ggtgaggccc gccgacgcgg cctaagacct gatgcaggca aaaacaagct acacgtccaa
     8101 aaggtcctcg ctggcgaatt ccgccttgtc ggaaatgaag gcaaatcggg cctcggcctt
     8161 ggtccccatc aggcgttcga ccgaatcggc ggtgccggca cggtcgtcgg ccaacagcac
     8221 cacgcggagc atcgagcgct tggccgggtc catcgtggtt tccttcagtt gcgccggcat
     8281 catttcgccg agacccttga agcggccgac atcgaccttg gcgttggcgt tgaattcgct
     8341 cttcaagagc gcgtccttgt gagcttcgtc gcgcgcgtag atcgttttgc taccgtgggt
     8401 gaggcgatag agcggcggca ccgcgaggta gaggtggcct tcatcgatca gccgcggcat
     8461 ctgccggtag aagaacgtga tcagcagcga ggcgatatgc gcgccgtcga cgtcggcgtc
     8521 ggtcatgatg atgatgcggg cgtagcgcag atcctcttgc cggtaatgcg cgccggtgcc
     8581 gcagccgatc gcctgcatca aatcggcgag ctgcgcattg gcggtgagct tgtccttggt
     8641 ggcagaggca acgttgagga tttttccgcg caaggggaga atggcttgcg tcttgcggtc
     8701 gcgcgcctgc ttggcgctgc caccggccga gtcgccctcg acaatgaaca gctcggagcc
     8761 ttcggcggcg gtgttggtgc agtcggcgag cttgcccggc agacgcagct tcttcaccgc
     8821 cgtcttgcgc gagatttcct tctcggcgcg gcggcgcagc cgctcgtcgg cgcgctcgac
     8881 cacgaactcc aggagcttgc tggcctgcag cggattggcc gacagccaat ggtcgaacgg
     8941 gtccttgatc gcctgttcga cgatgcgctg cgcttcggcg gtggcgaggc gatccttggt
     9001 ctggccctgg aattccggct cgcgcacgaa caccgacagc atcaccgcgg cgccgaccat
     9061 gacgtcttcc gagacgatgc tggcggcgcg cttgccctgg ccgacgcgct cggcgtgatc
     9121 cttgaggccg cgcagcagcg cgctgcgcat gcccgattcg tgggtgccgc cgtcctgggt
     9181 cggcacggtg ttgcaatagg aggacatgaa gccgtcggca tcggcggtcc acgccacggc
     9241 ccattcgcaa gcgccgtggg cgccgacccg tccggacttg ccggagaaga tatccggatg
     9301 cacgagggtg tcggacgcaa cggcggcagc gagaaaatcc ttcaatccgc cgacgaagtg
     9361 aaacgtgtcc tcggcgggta catcctcgat acccttcagc agggccgggt cgcagcgcca
     9421 gcggatctcg acgccgccga acaggtaggc cttggagcgc gccatcttga acagccgctg
     9481 cggcttgaag gtggccttgg cgccgaagat atcggtatcg ggcttgaatt tgatacgggt
     9541 gccgcggcgg ttgttgatct tgccgaggtc ctcgagcttg cccttggggt ggccgcgctc
     9601 gaacgtcatg cggtagagtt tttggctgcg cgcgacttcg acctcgagcc gcgaggacag
     9661 cgcgttcacc acggacacgc cgacgccgtg cagaccgcct gatgtctcgt agaccttgga
     9721 gtcgaacttg ccgccggaat gcagcgtgca catgatgact tcgagcgcgg attttttcgg
     9781 aaattttgga tgcggatcga ccggaatgcc gcggccgtta tcggtcacgg tcaggaatcc
     9841 gtcggcgctc aattccactt cgatgaagct cgcatgcccc gccagcgcct cgtccatcga
     9901 attgtcgatg acttcggcga acaggtggtg cagcgccttc tcgtcggtgc cgccgatata
     9961 catgccgggc cgccggcgta ccggctcgag cccttcgagc acctcgatat cggctgcggt
    10021 gtagccggct tcggcggttg ccgcgcgcgg cgcggctttg agcggggcgc ggcctttcgg
    10081 ctccgaggcg ccgaacaggt cgttggcgga tttggctttg gcgttatttt tcaatggttt
    10141 gtccatatat tttcatgtgt tgcgcgcgcc tgggagcgcc acgaatcggt ttccctgact
    10201 atgccacgga tgagctgaaa aagtgacggc ggaggacgag gcgccgggcg gtgtcgccgc
    10261 ccaacccgta ccggtgggga ggccgtcgtt tcagacccgc gaagtccgga acacgatcac
    10321 cgcgaacagc gccatcgcca tgaccacaat cagggatccc acaatcgggg ccgcgataaa
    10381 cgaatccccc tttgggagca cgagggcgac gccggccgga aacaggatcg cgccgatcat
    10441 gtggagccag ccccgcacct tggcaagggt cgtggtcccg gcagccggaa ccagcctgta
    10501 gtacagccga acagaaacaa gagcacgcca ccgaccaggt tgagatgggc atgcgcgggc
    10561 ccgagcgcga agttttgctg gatgcccatt gcgatgccgg cgagcatccc gaccagaacg
    10621 ctcaggcaca tcatcagcgt agtttcgcct ggcgggctga aaagccattt gggagagccg
    10681 gggctcacgc accgtccccg tctttgtgac ttgatgtcgc cgagggggct ggcttacctg
    10741 atcttaggca ttggaaccat agaaaggttc aacgccttgc tcagacgggc tgaagggcca
    10801 ggggaggcgt ccgaaacatg gaagattttg cgcacgccct ggccgatttc gtgcgcgacc
    10861 acagggtctg ggccgctccg atcgttctgc tgctggcgtt cggtgaatcg ctggctttca
    10921 tatcgctatt gatcccggcc tggggcgcgc tggtcgcgat cggggcgctg atcggcgcca
    10981 gcggcatcag cttctggccg gtctggattg ccggcggcat tggcgccgcc gtcggcgact
    11041 gggtctccta ctggttcggc ttcaaataca aggaacaggt tgcgcggatg tggccgctgt
    11101 cgcgctatcc ggagctgctg ccgcgcggcg aggccttcgt gaaaaactgg ggcgtgccga
    11161 gtattttcat cggccggttt ttcggacctt tgcgcgcctc ggtgccgctt gccgccggca
    11221 ttttcgaaat gccgtattgg agttttcaat tcgccaattt cgtttccgcc ctggtctggt
    11281 cggcggcact gctgctgttc ggcgacgtga tcgcgcaaat cctcgaatgg ctgtggcggg
    11341 tggtctgaag cctccttgga ataaaccgcg ggcgggggtg ttaccccctc aaatagcgtg
    11401 cgttgcactg gcatggtcgt gatcgccttg cggccaaaac gctggtaaaa ttagccgcga
    11461 tcagacgcaa gaaagcggaa aaattctgca ccgaaatcat cgtctcgctg ggagaaaatc
    11521 atcatggaac taacacgacg tcacgcattg gccggcgccg ccgcgatcgc ggccacgcag
    11581 cttttaccat ccgtgcctgt gagagcagcc gccccggtgg cggacaagca ggcgccgagt
    11641 ttctatcgct acaaggtcgg cgacatccag gtcacggtgg tgtccgacgg caagaatgtc
    11701 ttcaagctcg aggattcctt catcaccaat gcgaagaagg atgaggtcaa cgccgcgctt
    11761 gagaaggcgt ttctgccgcg tgacatcatg tcgatctact tcgcgccgct ggtgatcaac
    11821 accggcggca agctcgtcgt gctcgacacc ggcaatggcg cgatcgccaa agccaacagc
    11881 aagggcgcca acggactgtt cgccgacaac atggccgcgg cgggcttcga tgccaacgcg
    11941 gtcgatatgg tggtgatctc gcacttccac ggcgatcacg tgaacggctt gctgacggcc
    12001 gacggcacgc cgacgtatcc gaacgccgaa gtgctggtgc ccgcgaccga gtggaaattc
    12061 tggatggacg acggcgagat gagccgggct tcaggcccgc ggatgacggg cctgttcaag
    12121 aacaaccgca acatctttga ggcggctctc aaaaagaagg ttacgccgta tgagtgggac
    12181 aaggaggtcg cgccgggaat gctcgcggtc gagaccgcgg ggcacacgcc tggccacact
    12241 tcctatgtgc tgtcatcggg ttcggggaag gtgttcatcc agtcggacgt gaccaataat
    12301 ccgaatccgt ttgcgaccaa tccgggctgg catgcgttct tcgaccagga cggggatatg
    12361 gccgaaaaaa cccgccgccg ggtctacgac atggtggccg ccgaaaaact gcaggtgcag
    12421 ggcttccact atccgttccc tggcctcggc aatgtcgaaa aggacggcag cggttaccgg
    12481 gtgattccgg cgccgtggaa tccgacgatt tgattcgtca ttccggggcg tgagctcgcg
    12541 acaaaattga cgccttcgtc aattttgcgc tgagcgaacg agcccggaat ccataaccac
    12601 gacagggggt atggattccg ggcctgcgca tccaagtcgg ctgttgccga cttggacaat
    12661 gatgttgccg aactcgggta aacccgagtt cggttggcgc atcctggaat gacggcaacc
    12721 atcttgacga aaaccgggcg tggccttata gcccccgccc atgatcaacg ccgcgaccat
    12781 cctgattgct cgccgccgcc gtaccattgc ggtatgggcg gacatctgcg tttaagatat
    12841 cgcctctgcc gccggatccg gtccgcggca cttccttcca aaccagcgca atctgattcc
    12901 cggcggctcc ctcaaccaag caggagcagc catgttcacc cgacctttct ttgagcccga
    12961 tcgcgccgcg agcctcgcct tcgcggacgc gcgcggcttc ggcacggtct gcgcctgtga
    13021 tggcggcaag ccggtcgcat cctcgctgcc gttctatctc agttatggcg acgacggcac
    13081 gccacacgct ctgtttcatg tcgcccgtca aaatgcgctg gcgacgctgg cggacgggaa
    13141 atcatcctgg ctgatcgccg tcaacggcgc ggatgcctat gtttcgcctg actggtacgc
    13201 ctcgccggat caggtgccga cctggctcta tcaggccgtg catctcaccg gcaccgtgcg
    13261 gaggctttcc gatcacgaac tcggtccgca cctcgacgcg ctgagtgcga agtttgaaag
    13321 ctggctcatg ccaaaaccgc cttggatgtc gtcgaagatg acggccgctc ggctcgatgc
    13381 gatgaagaag gcgatcgtgg gtctggtgat gacggttgaa gagatcgagg gcagtttcaa
    13441 gctcaaccag cacaaatccg acgtcgatca tgcggccatt gccgatgcgc tggctttaca
    13501 agccgacgcc ggcgcgcaac agctcgcacg caccatgcgc acgatgcgcc cgcaagcctt
    13561 tacggatgaa acggtacagc tcgaaaggag cgtgtgatga gcacgaagaa gaagatcggc
    13621 attctcggcg cgtccggcta caccggcgcc gacgcggtgc gcctgctggc gcggcatccc
    13681 gatgccgaaa tcgtcgcgct caccgccaat acccatgccg gcaaggccat ggacgaggtg
    13741 ttcccgcatt tcttcatgct ggacctgcca cggctcacgg aatgggagaa ggtcgactgg
    13801 accaagctcg atgcggtgtt ctgcggactg ccgcacggca cgacgcagga gatcattgcg
    13861 gccgtcctgg ccgccaatcc ggcgatcaag atcctcgaca tgtcggcgga tttccggctt
    13921 cgcgacgtga gcgaatacgc gcaatggtac ggccatgagc accgcgcgcc aaaacttcaa
    13981 ggtgaagccg tctatggctt gaccgaattc tatcgcgaga acatcaccgc ggcgcggctc
    14041 gtcgcctgtc ccggctgcta tccgacggcg gcactgctgg cgctggtccc gatcgcgaag
    14101 gcaaagctga tcgacgtcga cgatatcgtc atcgacgcga aatcaggcgt caccggcgcc
    14161 ggccggggtc tcaggcagaa cacgctgttc agcgaagcag gcgaggggat ttcgccctat
    14221 tcgattgcct cgcatcggca ttcgccggag atcgagcagg agatcggcgt cgccgcaggt
    14281 aaaaaagtga cggcaaactt cacgccgcat ctcattccga tgagccgggg cgagctctgc
    14341 acgtcctacg tcaagctcaa cggcgcgtgc gcggacgatc tgcgcgcagc cctcgcggag
    14401 gcctacaagg acgagccgtt cgtgcatgtc gccaaaaagg gcgtgctgcc gcagacgcag
    14461 aacgtgcgcg gctcgaatta tgtgcagatc ggcgttttcg ccgaccgcat caagaaccgc
    14521 gcgatcatca tttcggtgct cgacaatctg gtgaagggct cggcggggca ggcgatccag
    14581 aacatgaacc tgatgctcgg gtttcccgag accacgggac tggagcagat cgcgctgttt
    14641 ccgtaagccg tcagttcgaa caaaacagcg gaagccggac cgagatccgg gcgccgcccg
    14701 atggggcttg cccgatcgac aggctgccgc ggtgggcctc gaccaggccg cgcgcgatcg
    14761 caaggcccag tccggcgcca ccggtgccgc ggttgcgcga tgtctccagc cgactgaacg
    14821 gctcgagcat cgcctgccgc tgctcgggcg gaatccctaa gccttcgtcg tcgaccgtga
    14881 ggtcgatggc gttgccgacg atcacactgc gaagatgcgc agctccgccg tatttgaggg
    14941 cattgtccgc gaggttggcg acgacgcgtc gcagcgccag acggtcgccg agaacgatcg
    15001 gaacctgctc ccattgaccc cgctgataat cgatgcgcgc gccgccgctt cgccggtcgg
    15061 cgacttccgc accgataata tcgtcgatct cgaccatctc ttcggccagc tcgccggcgc
    15121 cggcgcggct cgccagcagc gcgtcgtcga gcaaaacgat catgtcgttg atgtcggcga
    15181 tagcgcggtc gcgttcggtc gcgtcgctga tcttttcggc gcgcagccga agccgcgtcg
    15241 cataggttcg cacatcgtgg gaaattccgc ccaacatcac catgcgcgag cgcaagagct
    15301 ccccgagccg gttttgcaaa cgattgaacg catcgatcag cgcccggatt tccggcgcgc
    15361 tgcgtttggc ttccggcagg gctaccggat caagcgatag atcgacgcgg tcgaccgcct
    15421 cggcgaggcg cgccagcggc ttggtctcgc ggtgcatgac aaagagcgcg agcaaggcaa
    15481 tcagggagcc gaaaaggccg gcgccaaatc cgaccggcag acccagcgga cccaccagaa
    15541 ttctgttgcc gatttcgatg atcagcgttt ctccggtcgc aagaccgacg cgtacgtcga
    15601 tcgtcgtcgg aacgaggcgc gagaacaatc gccagcgctc gccggcgtcc gagaaattga
    15661 cgctgacggg gcggccgccg agcggctgca gagactgctc gatcgcgcgc gcgttgaggc
    15721 gtggcaggat ccgcggcgcg gtcgctccga tcgtcgcggc gtcatcgata cgcgccgaaa
    15781 acgtttccga actcgccgcg cgaagcacca gcgcccgctc cgggccgggt gtgcgttcga
    15841 tcagttcgac gacagcggcg atatggcccg gaacgggcag ggtattgtct tcggccgtat
    15901 gggcgcgtgc aaggtaatac aacgcgatgg cggagatcca ggccatgccg agcccgacca
    15961 cgacgatcag tgcgatcctt gccgcgaacg tcatgcgcag gatcatgtca gcgggctcac
    16021 atgggcggtg aacaggtaac cgccgttgcg gacggtgttg atcaggttgg agccgggact
    16081 cgcaccgtcc agcttcttgc gtaaccgcga gatcagcata tcgacggtgc ggtcgaacgg
    16141 atcggcggat cgtccgcgcg tccagtccag aatttggtcc cgtgtcagca cccggcgcgg
    16201 ccgctggaca aaacagacca gcagttcaaa ttcgccgctt gtcagattga ccgcattgtt
    16261 ctcgccggcg ctcagtccac gggcgtcgat atcgatgacg aaggcgtcaa agcggtagcg
    16321 gcggttctgt gcgggctgcg gcgccgtggg ttgtgtccgg cgcatgatgg cgcgggcgcg
    16381 ggccagcagt tcgcgcgagc tgaagggttt tgcgagataa tcgtcggcgc ccatctcgag
    16441 accgaccacg cgatcgattt cgtcgctttt ggcggtcaac atcaggatcg gaatcgggtc
    16501 gccggtccgg agccggcgac agatcgcaag accgtcctcg ccgggaagca tcagatcgag
    16561 aatgatgaga tcgggcctga cgcgctgcaa gacgacgtcc atggctttgc cgtcctcggc
    16621 ggtctcgacg tcgtatccct catgaagcag gaaatcgcgg accagccgcc tgatctcggg
    16681 gtcgtcttcc acaatcagga tggtcggctt cggcgtcgtc atgtcgtgaa tgtcggacaa
    16741 tcgaacggcg gcggcaagcc gagccgccgc attgttacaa actgttacgt tgcgtcgaca
    16801 aatcgccaac aaacgtctcg cgccgttgaa attgcgctga cgtggcgcgg ccagggtctc
    16861 ggcacggtcg ggctcacgcc gagcgccgcc ggtacaacag gagagccaac ccatgaagaa
    16921 gcttttcgcc atcgccgcga ttgtcggagc cttgtccgcg gcggcaattg tcgatgccaa
    16981 tgccggttcg cgcagcggat cgatctcggg cccgcgcggc accacgacgt ttgccggctc
    17041 gcgatcctgc gcaggcggcg cctgctcgtc tcagggcagt gtcaccgggc cctatggcgg
    17101 cacgatatcg catcagggtt cgagcagctg ttcgggcggc acctgctccg gcggcggcac
    17161 cgtcaccggc ccccgcggcg gctcggtcac ttacaacggc acggtcacac gctgacgagg
    17221 cgccgcggct ggtgcaggca gtgctgccgc cagccgctgg ccttgcctga ggacacggtc
    17281 atgaatcaac tcgcaggttc cttggctgcc agctcgcgcc tgatgctgtt ggcaaccgca
    17341 ctgggcttca tgggcctcga atattttctt ggtcgactgg cccatcatga tacccacgac
    17401 ccctttgaaa gcgccgcatc gctgggtgtg gcggcgggcc agtcggtcgt tcgggccgtc
    17461 gaggccacgc tgctggctat tccttttgcg ttcgtctatc agcaccggct gttcgacttc
    17521 tcgcagacca cgccgctggc gctggccgcg ctgttcgtgg cgatggagtt cgcttattat
    17581 tggcagcatc gcgcctcaca ccggatccac tggatgtggg cgacccatgc ggtgcatcat
    17641 tcggcaacga agctgaattt caccgccgcg gtcaggctcg gctggaccgg caacatctcg
    17701 ggaaatttcc tgttcttcct gccgttggtc tggttcggat ttcatccctt cgccgtgatc
    17761 ggcatgctcg gcgtcaacct gttctaccag ttcttcatcc acaccgagct cgtgccccgg
    17821 ctcggtccgc tggaatggat tctcaatacg cccgcccatc accgcgttca ccacgcctcg
    17881 aatccggcct gcctcgacaa gaactacggc ggcatcctga tcgtgttcga ccggatgttc
    17941 ggatccttcg cggaggcgcc gccccatgaa gcgctgcgtt acgggcttgt ccacggcacc
    18001 agcagttaca acccggtcag gatcgcgctt ggcgaatggc gcaacatgtt tcgcgatttc
    18061 gcccgcgcaa ccacgctgcg cgcacgggtc aagacgctgc tcgggc
//
LOCUS       DASWGM010000126         3680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2368529,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000126 DASWGM010000000
VERSION     DASWGM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368529"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VGA15_03935"
     CDS             complement(<1..1005)
                     /locus_tag="VGA15_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9586876.1"
                     /translation="MQQIRVCTYDGPGAQPVIRSVPWPKIPKQAALIKVGACGVCGTD
                     LHILKGHWPKQLPWPFTLGHEIGGVLVEVGSDFKEDFMSKPLGVGSKVMIPPLMPCGQ
                     CYYCIHYPESANKCLTPVYYGRYLGFDKAPHLWGGWAEYVYVDLEMLPGTKIYKLPDD
                     MSLRLGALSEPLTSCIRAFNRATRAGGFKWGDTVVIQGAGPIGILAVAAAQEMGAGRV
                     ICVGAPETPRLALARKFGAEATVNIEEVTTPEARIKAVRDIVGGFGADLVMDCSGHPS
                     AGPEGIEMLRDGGTYVEMGQFTDAGSIETSWHRICTKDLNVLGSWGFTGNDLPLGVDM
                     L"
     gene            1257..1625
                     /locus_tag="VGA15_03940"
     CDS             1257..1625
                     /locus_tag="VGA15_03940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9586877.1"
                     /translation="MHNRWFMLAVLFAVRLTMGFQFQSVAAVAPLLGRDFGVGLADIG
                     ILIGLYFTPGVALALPGGAIGQRFGDKRTVLGALLLMLSGGLAASPQAGQRTAPPTFL
                     SPQHSKPLPASFARASQNPR"
     gene            1692..2306
                     /locus_tag="VGA15_03945"
     CDS             1692..2306
                     /locus_tag="VGA15_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9586878.1"
                     /translation="MALAHKRSILPAAEERRRFQRVRVHLLGRYMLPDRREFPCQIIN
                     MSPGGLALLAPGIGNVGDRVIAYLDHIGRVEGKITRIIDNGFAMTVGATARKRDKLAA
                     QLTWLANRDILNLPEDRRHDRIIPRNPISLLTLEDGTKMTCRVIDMSLSGAAIAAENR
                     PPLKSLVLLGKVQSRVVRNLEEGFALEFVHEQLAETLEDSVTAR"
     gene            complement(2321..2473)
                     /locus_tag="VGA15_03950"
     CDS             complement(2321..2473)
                     /locus_tag="VGA15_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586879.1"
                     /translation="MIPAGTGHWFTGIEDHLDDIRVRIDPDEVTPLKSEAKIAGIFVQ
                     AGTARQ"
     gene            2947..3573
                     /locus_tag="VGA15_03955"
     CDS             2947..3573
                     /locus_tag="VGA15_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEX9586880.1"
                     /translation="MFGFRGQGKGLAVVAILFGINAPASAGEERALYASLGDTTRSPI
                     GWVEFCADNPGDCHGGTTQPRDIVMSQTAWRDLLQVNRWVNETVKPMTDMDHWGVIEK
                     WSLPTDGYGDCEDYVLMKRKMLIDAGWPREALLITVVRDKKGEGHAVLTVKTDKGEFI
                     LDNQNENVVAWTETGYRFVKRQSQSDPNVWVSLGDGRPAVATASAHDR"
BASE COUNT          687 a         1182 c         1086 g          725 t
ORIGIN      
        1 gagcatgtcg acgccgagcg gcaggtcgtt gccggtaaaa ccccatgagc ccagaacgtt
       61 gaggtccttg gtgcagatgc gatgccatga cgtctcgatc gaaccggcat cggtgaactg
      121 gcccatctcg acataggtgc caccgtcgcg cagcatttcg atgccttccg ggccggcgct
      181 cggatggccc gaacaatcca tcaccaggtc ggccccaaag ccgccgacga tgtcgcgcac
      241 ggccttgatg cgcgcttccg gcgtcgtgac ctcttcaata ttcactgtcg cttccgcgcc
      301 gaacttgcgc gccagcgcaa gccgcggcgt ctccggcgcg ccgacgcaga tcactcgccc
      361 tgcccccatc tcctgtgctg cggccacggc caaaatcccg atcgggccgg ccccctggat
      421 cacgacggtg tcgccccatt tgaatccgcc agcgcgcgtg gcgcgattga aggcacggat
      481 gcaggaggtc agcggctcag ataacgcgcc gagccgcagc gacatatcgt cgggcagttt
      541 gtagatcttg gtgcccggca gcatctcgag atcgacatag acatattcgg cccagccgcc
      601 ccacagatgc ggcgccttgt cgaagccgag atagcggccg taatacaccg gggtcaggca
      661 cttgttggcg ctttcgggat agtgaatgca gtaatagcac tgaccgcacg gcatcagcgg
      721 tgggatcatc accttcgatc ccacgccaag cggcttgctc atgaaatctt ccttgaagtc
      781 cgagccgacc tcgaccagca cgccgcctat ttcgtggccg agcgtgaacg gccacggcag
      841 ctgcttcggc cagtggcctt tgaggatatg cagatcggtg ccgcacacgc cgcaggcgcc
      901 gacttttatc aatgccgcct gcttcgggat cttgggccaa ggcacggagc gaatgaccgg
      961 ctgcgcgccg gggccgtcat aggtgcagac gcgaatctgt tgcatggaat ttcccccgtt
     1021 gtgttttgtt cttgacgatg ttcggaccga tcatagagct gccgtcaacg gcgtcacaac
     1081 atcgccattc gtcgctcctc cgcccttcgc gattgttgat tgaatttatt cgggcgcggc
     1141 gagcctatgt gaactccgcg ccgatgccaa tcttgccgtg tcgcaatcga tgcacggcga
     1201 ttgacaggcg cagcaccgcg tccgaggttg cgcggtgatt tcaatggagt gaggtgttgc
     1261 acaaccgctg gttcatgctt gccgtcttgt tcgcggttcg cctcaccatg ggctttcagt
     1321 ttcaaagcgt cgccgccgta gcacctctgc tcggccgcga cttcggcgtt ggactggccg
     1381 acatcggaat cctgatcggc ctgtatttca ctccgggcgt cgcgctggcc ttgccgggcg
     1441 gcgccatcgg acaaaggttc ggcgacaaga ggacggtact cggcgccctg ctgctgatgc
     1501 tgagcggggg ccttgcggcg tcgcctcaag ctggccagcg tacggccccg ccaacgtttc
     1561 tatcgccgca acactcaaaa ccacttcctg cgtcatttgc ccgggcttcg cagaaccctc
     1621 gctaactcct cattaaccct atcggccgta gggtctggcg acaccgagca gccggttgtc
     1681 gggtggcgaa gatggcgttg gcgcacaaaa gatcgatact tccggccgcc gaagagcgcc
     1741 gccgctttca gcgggtgagg gttcatctgc tcggccgcta tatgctgccg gaccgccgcg
     1801 aatttccatg ccagatcatc aatatgtcgc cgggggggct cgccctgctg gcgcccggca
     1861 tcggcaatgt cggcgatcgc gtcatcgcct atctcgacca tatcggccgg gtcgaaggca
     1921 agatcacccg cattatcgac aacggtttcg ccatgaccgt gggcgccacc gcgcgcaagc
     1981 gcgacaaact cgctgcccag ctaacctggc ttgccaaccg cgatatcctc aatctgccgg
     2041 aagaccgccg ccacgaccgc atcatcccgc gcaacccgat ctccctgctc acacttgagg
     2101 acggcacaaa gatgacctgc cgggtgatcg acatgtcgct gtccggtgcc gccatcgcgg
     2161 cggaaaaccg gccgccgctg aaatcgctgg tcttgctcgg caaggtccag tcgcgggtgg
     2221 tgcgaaacct cgaagaaggt tttgcccttg agttcgtcca cgagcagctg gccgagacgc
     2281 tcgaggacag cgttaccgcg cggtaaagcc cggcaccggc tcattgccgc gccgtgccgg
     2341 cttggacaaa tattcctgcg attttcgcct cgcttttgag cggcgtcact tcgtccgggt
     2401 cgatccgcac cctgatatcg tcgaggtggt cctcgatccc tgtaaaccag tgaccggtgc
     2461 cggccggaat catgacgacg tcgccggcct tgatcccgca ggcgatgcct cagaaccgtt
     2521 gtttccggac cccttgaatt cgacgaccgg gcgcacggac cccgcgcttt gccaggtttg
     2581 ccttgcatcg ccaaagacag cgttaccgcg cggtaaagcg ccccgccctg ccattcccac
     2641 attccgtagc ccgccagcgg cctcttcgcc gcggatgccg ccctttcgcc gttgcgcgcc
     2701 tgcttcactt taagttaacg cgtcgggctt ttgccgccgc aagttccgca tcgacgggct
     2761 ttgccgcgtt aatacgtaaa atttgaatca attgtagttg tttcaaattt taatcgaatt
     2821 ttactcaagt atagatcgaa ttaaccgaag cttttactcg tattcgtctt aaatgtactt
     2881 ggcttactta gcggcggcgc aaaacctttg tgcgaaacat ggtcccaaca agaaacgggg
     2941 gccgcaatgt ttgggttcag gggacagggg aaagggttgg cggttgtcgc catcctgttt
     3001 ggtatcaacg cgccggccag tgccggcgag gaacgagctc tttatgcgag cctgggcgac
     3061 accacgcgct cgccgatcgg ctgggtcgaa ttctgcgccg acaatcccgg cgattgccac
     3121 ggcggcacga cgcagccgcg cgatatcgtg atgtcgcaga cggcatggcg agatctgctg
     3181 caggtcaacc gctgggtcaa tgaaaccgtc aagccgatga ccgacatgga tcattggggc
     3241 gtgatcgaga aatggtcgtt gccgacagac ggctacggcg actgcgagga ctacgtattg
     3301 atgaagcgca agatgctgat cgacgccggc tggccgcgtg aagcgctgct gatcacggtg
     3361 gtgcgcgaca agaagggcga aggccacgcg gtgctgaccg tcaaaaccga caagggcgag
     3421 ttcatcctgg acaatcagaa tgaaaacgtc gtggcctgga ccgagaccgg ctaccgcttc
     3481 gtcaagcgtc agtcgcagag cgatcccaac gtctgggtct cgcttggcga cggccgcccc
     3541 gcggtcgcca ccgccagcgc ccacgatcga taacaggaaa taacaggaca cgcgatccgg
     3601 tcacatcccc acccctcccc gtcccagacc ggatcgcgcg cggccagctc tcccccaaga
     3661 gctggccgca acttttttgg
//
LOCUS       DASWGM010000127         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_11808,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000127 DASWGM010000000
VERSION     DASWGM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11808"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VGA15_03960"
     CDS             <1..679
                     /locus_tag="VGA15_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586881.1"
                     /translation="AVRVYSFETGGNGNHDMQSGLSASRPGSRAISTAIGYNQLLTTN
                     TVELLAEQGPEFVRALAEKAAQLSGAPRQAMDRKIAVLKRMVAYARSVPDEWAQHEKI
                     GDTPQGWALHAMVLDIDVGPLLQTHKLLTSVIFARAKGFSHPLTAAELEMMNLTGDGT
                     GLDMVTMPQALREQVPTSNFFQRGGYERNPVAIRNNTVAKLLDVTDRRMDSNSNLPGA
                     KELAAAF"
     gene            complement(843..1148)
                     /locus_tag="VGA15_03965"
     CDS             complement(843..1148)
                     /locus_tag="VGA15_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586882.1"
                     /translation="MTRFSTRTFLIAAALIALTTSAFAEAATPTPWVLTPDMGYGYDK
                     EGKTLSYKMGTNNAKLLLKGAKKVPKGTLFFIGENGQLYMRTGPFLEGDGKFMFGSN"
     gene            1380..1859
                     /locus_tag="VGA15_03970"
     CDS             1380..1859
                     /locus_tag="VGA15_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586883.1"
                     /translation="MSHVMKICFGVALLLVTLVRLAHADDFKLSNNQRISCSRGLSAG
                     KLSTATCKSYAYLFNIKTTEYFRCQVSLALTRDNKEVINVQTDGGCTKKPRIFDTDSN
                     YSFDATETEPPNTNSFFGPGGYAVWASDNTAQKVRGCIIISSGLGSDISKCLDMTFQ"
     gene            complement(1906..2448)
                     /locus_tag="VGA15_03975"
     CDS             complement(1906..2448)
                     /locus_tag="VGA15_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEX9586884.1"
                     /translation="MTIFRRAPACLVASGVFFLGLFYAAGTVTYAAPINNELMLVNAQ
                     TGKCLTIAGGVSTANNVESVQFDCDSDPSRRWMLREMSGGNIYQIKNVQTGKCLTIAG
                     GLSTDDNVTALQFDCDSDPSRTWRISDVTGSGVNQIRNVQTNKCLTIAGGVSTDNNVR
                     ALQFRCDADPSRRWTIRLKL"
BASE COUNT          489 a          784 c          758 g          523 t
ORIGIN      
        1 ggcggtgcgg gtctattcgt tcgagaccgg cggcaacggc aatcacgaca tgcaatcggg
       61 attgagcgcc tcgcggccgg gctcgcgcgc gatctcgacc gccatcggct acaatcagct
      121 gctcaccacc aacaccgtcg aattgctcgc cgaacagggc cccgaattcg tccgcgcgct
      181 tgcggaaaag gccgcgcaat tgtcaggcgc gccgcgccag gcgatggatc gcaagatcgc
      241 ggtgctgaag cggatggtgg cctatgcgcg ttcggtgccg gacgaatggg cgcagcacga
      301 gaaaatcggg gatacgccgc agggctgggc gcttcatgcg atggtgctgg acatcgatgt
      361 cggcccgctg ctgcagaccc acaagctgct cacgtccgtc atcttcgcgc gcgccaaggg
      421 ttttagtcac ccgctgaccg ccgccgaact ggagatgatg aatctgaccg gcgacggcac
      481 cgggctcgac atggtgacga tgccgcaggc gctgcgcgag caggtcccga cctcgaattt
      541 tttccagcgc ggcggttacg agcgcaatcc ggtcgcgatc cgcaacaaca ccgtggcgaa
      601 actgctcgat gtcaccgacc gccggatgga cagcaacagc aatctgccgg gcgcgaagga
      661 actcgcggcg gcgttttaaa cttctcgtcg tcccggccaa gcgctcttcg cgcgcgagtc
      721 gggacccata cggcgcggag ccccgtttcg gcagtggggt agaagccttc ctgaattacg
      781 gacgccaggg gttatgggtc ccggcgttcg ccgggacgac tcgcgttgag gctaagccct
      841 gttcaattgc tcccgaacat gaacttgccg tcgccttcaa ggaacggtcc ggtccgcata
      901 tagagctgtc cgttctcgcc gatgaaaaac aacgtgccct tcggcacctt tttcgcgccc
      961 ttcagcagca gcttggcatt gttggtgccc atcttgtagg aaagtgtctt gccttccttg
     1021 tcgtagccat agcccatgtc gggcgtcagc acccagggcg tcggcgtggc ggcttctgca
     1081 aatgcggatg tcgtcagcgc gatcagcgcg gctgcgatca aaaaggtcct ggtggaaaat
     1141 ctcgtcatca cgcgtcctcc cgcaaccggt cttcaaagct ggtcggtttg ccatgcttga
     1201 acaaaccacg aacgccattc acccgtcaat atgaccggcc cattcatcgc agcgcgtcgc
     1261 cttagacttt agactttatg atttcagact ttgtgattcc ccgcatccga cttcgcgact
     1321 atctttcggt ttcagtctag aaccgttttt gaaaaaatac gtcacggggg atgattcgga
     1381 tgagccacgt catgaagatc tgctttggtg tcgcgctgtt gctggtgacg ctggttcgcc
     1441 tcgcccatgc cgatgacttc aagctcagca acaatcagcg gatttcctgc agccgcggct
     1501 tgagcgccgg caagctcagt accgcgacct gcaaatccta cgcctacctg ttcaacatca
     1561 agactacgga atatttccga tgccaggtca gcctggcgct gacgcgggac aacaaggaag
     1621 tgatcaacgt ccagaccgac ggcggctgca ccaagaagcc tcgcatcttc gacaccgatt
     1681 ccaactactc gttcgacgcc accgagaccg agccgccgaa caccaattcg tttttcggtc
     1741 ccggcggcta tgcggtgtgg gccagcgaca acaccgcgca aaaagtgcgc ggctgcatca
     1801 tcatctcgtc cggcctcggc tccgacattt cgaaatgcct cgacatgacg tttcagtgat
     1861 ttgcgggcag gcgtaatggc catgatatgc gacgattggc cctcgctaca gcttcagcct
     1921 gatcgtccag cggcgtgacg gatcggcgtc gcagcgaaac tgcagggcgc ggacgttgtt
     1981 gtcggtactc acgcccccgg caatcgtcag gcatttgttg gtctgcacgt tcctgatttg
     2041 gttgacgccg ctgccggtca cgtcgctgat cctccaggtg cgcgacggat cgctgtcgca
     2101 gtcgaactga agagccgtca cgttgtcgtc ggtgctcaag cccccggcaa tcgtcaggca
     2161 cttccccgtc tgcacgttct tgatctggta gatgttgccg ccgctcatct cgcgcagcat
     2221 ccagcggcgt gacggatcgc tgtcgcagtc gaactgcacg gattcgacgt tgttggcggt
     2281 gctcacgccg ccggcgatgg tcaggcactt gccggtctgg gcgttgacga gcatcagttc
     2341 gttgttgatc ggggcggcgt acgttacggt gcccgcggcg tagaacagcc cgaggaagaa
     2401 cacgcctgac gcaaccaggc aggccggggc gcgacggaag attgtcatga tgtctctcct
     2461 taagagcggt tagtggcgag atgaattctc gacaaattgc cctgtcgatg gctaccacaa
     2521 cctaaccgag cattttgtga agtggcccac aggc
//
LOCUS       DASWGM010000128         8441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1532340,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000128 DASWGM010000000
VERSION     DASWGM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532340"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VGA15_03980"
     CDS             complement(<1..656)
                     /locus_tag="VGA15_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEX9586885.1"
                     /translation="MSISLHPDTPEGRTLGGSPAATVAPGVEPSTKIRSRLFTKYVAL
                     FVAVVGIALLSNGIFEVFFYYREHKAALIRIQHEQAEAAAAKIGQFVKEIESQLGWTT
                     QLPWSAGSLENRRFDALRLLKQVPAITELAQLDSTGKERLRVSRLAMDVVDSGLDLSK
                     DPKFTEAVAHKVYYGPVYFRRESEPYMTIALAGTRKDAGVSIAEVNLKLIWDVVSQIK
                     V"
     gene            complement(750..3845)
                     /locus_tag="VGA15_03985"
     CDS             complement(750..3845)
                     /locus_tag="VGA15_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MexW/MexI family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HEX9586886.1"
                     /translation="MVWTDIFIKRPVLSVVVSLLILLIGFRAATTLPIRQYPKLSNTV
                     VNVTTVYPGASADLIQGFITTPIEQAVASAEGVDYMTSSSVQGTSTIQVYIKLNFDPN
                     QALTEVLAKVNSVKYLIPKESNDPIVTKTTGQTTAVMYLGFSSEVLSGSAISDYLTRV
                     VQPVLSTVDGVASADILGGQTFAMRLWLDPVRMAGRNVSPDDVSAAIRANNFQAAAGQ
                     SKGYLTVSNVSTNTDLKNLDQFKRMIVKSKDGGFVRIEDIATVELAAQSTDASVAFNG
                     EHAIFIGVQATPQGNPLTLVKGVRGLFPELERNLPPSMKMKVAYDSTKFIQSSIDEVE
                     KTLGEAVIIVVVVIFLFLASFRSVIIPVVTIPLSLIGVCSLMLIAGFSFNLLTLLAMV
                     LAIGLVVDDAIVVVENIHRHLEEGKPPVQASLQGAREIVGPVVSMTITLAAVYAPIGF
                     LGGLTGSLFREFAFTLAGSVIVSGVIALTLSPMMCSVFLKSAEEGRFAKIVNKVFGSM
                     TRWYGRQLDRSLDYRPITGLFALTILGLVGFLYMHTSKELAPEEDQGIVFSVIKGPKY
                     ANIDYLDFYGEKLDKVFAGFPETDLRFALNGINGPQNGIAGMLLKPWDERERSSIKLK
                     PLVQAQLSKLEGVNAFAFNLPPLPGGPGGLPVQMVINSTNGFQSVYEEMLKLKAAARK
                     SGLFIVSDSDLDFNQPVVRVKIDRSKASDLGITMASIGNTLATLLGGNYVNRFNLEGR
                     SYQVIPQVPRDRRLSPEALGGYYVPTATGQQVPLSTVVSIETATDPNALTHYNQLNSA
                     TFQAVPMPGVTVGQAVDFLESQAKNLPAGYSHDYLADSRQYVQEGNQLAITFAFALII
                     IFLVLAAQFESLRDPLVIMISVPMAIVGALIPLFFGAATMNIYTQVGLLTLVGLISKH
                     GILMVEFANELQLNEGLDKRSAIEMAARVRLRPILMTTAAMVTGLLPLLTASGAGAAS
                     RFSIGLVVVAGMSIGTLFTLFVLPAVYVAIATDHRASAESQRSKEIADFDLGSKALKP
                     T"
     gene            complement(3986..5179)
                     /locus_tag="VGA15_03990"
     CDS             complement(3986..5179)
                     /locus_tag="VGA15_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9586887.1"
                     /translation="MNIVTEANISGKAITDKPRARPIRMVRWFIIVGLLLALLVGGLV
                     WFNYFRGQMIAQFFANNKPPPSSVSIAEAKSEVIPNLLTAVGGLAAVHQVDVTSDVSG
                     RITDIMFTPGATVKAGNPLVQLFDAPDQADLANFKAQATVAQLSLDRAKQLASRQFGP
                     QATVDSAQAAYDQANAGIAKSEAIISQKLVRAPFDGELGVRHVEVGQFLTAGTQIVTL
                     TDLSTLYANFTVTEKDSAALKVGQTVRILVDAYPGRTFEGKITAIEPQIATDTRNIRV
                     QATLDNPDHILKPGMFATTTVVLPDKPAVVTVPETAVDYTLYGDSVFLLTEKKADDGK
                     TSLTAVRTFVRTGDRIGGRAEILNGLKPGDRVVAVGQLKLQSGAAVAISTDAAPPIPA
                     NPPRY"
     gene            complement(5181..5885)
                     /locus_tag="VGA15_03995"
     CDS             complement(5181..5885)
                     /locus_tag="VGA15_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9586888.1"
                     /translation="MSTLRMTGDLRRQLILSAAKRCFARHGFAGTTTKSVAAAASISE
                     GLLFKHFPTKSALYAEILAEECEADPALHRLLGLEPSTETLAILIREMVRHFREVADA
                     PDQQEAQRLRLMITSHLDDGEFARLIYEKIGNLIGPVFTTSLERAVAAGDASRIGSDP
                     LNLFWFAHHTVLMATLARLPSVPCLPYGNAADFERQICEFILRGIGLNETAVVSHLDR
                     ELSPDPGQQPVTAEGA"
     gene            complement(6069..6659)
                     /locus_tag="VGA15_04000"
     CDS             complement(6069..6659)
                     /locus_tag="VGA15_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9586889.1"
                     /translation="MSQANLLERRSVVSHLLAERNDAIVVGGLGASTYDIAAAGDHDR
                     NFYLWGAMGGAVMIGLGMALAQPKLPVVVITGDGEMLMGMGSLATVSLQKPANLTIVV
                     LDNEVYGETGGQASHTASTVDFVGVARSCGISDARAIATMADIGTFASSMQDVSSGPR
                     FASIKIDSANLERVLSSRDGQFTVTRIRGALGFGPI"
     gene            complement(6656..7201)
                     /locus_tag="VGA15_04005"
     CDS             complement(6656..7201)
                     /locus_tag="VGA15_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEX9586890.1"
                     /translation="MHARTDAPPETAASWPEDIFAILQRFDVRQVPYVPDAGHSELIE
                     RVLGASSMRAVPLTTEEEGVALLAGAWAGGQRGVLLMQSSGVGNCVNMLSLTQIFRFP
                     FLTLVTMRGEWGEFNPWQVPMGSSTAAVFELSGIKVLRASHAEEVREVVEAAAGQAYN
                     ACTPTAVLLSQRLIGAKVFTK"
     gene            7315..>8441
                     /locus_tag="VGA15_04010"
     CDS             7315..>8441
                     /locus_tag="VGA15_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9586891.1"
                     /translation="MNANARELAANFDIEKLTGEFYADPYPTYRALREHEPVKLLPNG
                     SRFLTRYDDLVSAYKNTKLFSSDKRKEFLPKYGDSLLYEHHTTSLVFNDPPAHTRVRR
                     LIMGALSPRAIAEMEPDLIALVDRLLDTIAAKGSCELIGDFASAIPVQVIGNLLDVPM
                     EEREPLRDWSLAILGALEPVIGPEVFARGNKAVKDFLSYLETLVARRRTKPGNPERDV
                     LTRLIQGEDNGERLTETELLHNCIFLLNAGHETTTNLIGNGLVALSENPAEKNRLIEN
                     PGSIKTAVEEILRFESSNQLGNRMTTAQVELSGITLAAGTPVTLCIGAANRDPAQFAK
                     PEHLDIGRSPNRHLAFGTGAHQCAGMALARLEGAIAMSRFLA"
BASE COUNT         1698 a         2621 c         2530 g         1592 t
ORIGIN      
        1 accttgatct gggagaccac gtcccagatc agcttcagat tgacctcggc aatgctgacg
       61 ccggcatcct tgcgggtgcc ggccagcgcg atcgtcatgt agggctcgga ctcccggcgg
      121 aaatagaccg gcccgtaata gaccttgtgc gcgacggctt cggtgaattt cgggtccttt
      181 gagaggtcga gccctgagtc cacgacatcc atggcgaggc gcgatacccg caaacgttcc
      241 ttgccggtgg aatcgagctg cgccagttcg gtgatggccg gcacctgctt caacagccgc
      301 aaggcgtcga accgcctgtt ctccagcgag ccggcagacc agggcagctg ggtggtccag
      361 cccagctggc tttcgatttc cttgacgaat tggccgatct tcgccgctgc cgcctcggcc
      421 tgctcgtgct gaatccggat cagggccgcc ttgtgctcgc gataatagaa gaaaacctcg
      481 aagatcccgt tggatagcag cgcgatgccg accaccgcga caaacagcgc gacatatttg
      541 gtgaacagcc ggctgcggat ttttgtggat ggctccaccc ccggcgccac ggtcgccgcg
      601 ggcgatccgc ccagcgttcg cccctcgggc gtgtcggggt gaagggaaat gctcatccag
      661 gccgttttag caagaacagg tgccgctgtc tcttgctgca gtgcgggaag actattccct
      721 catctcgtcg gcacgggcga gcgcattgct caggtcggct tcagagcctt tgaaccgaga
      781 tcgaagtctg cgatctcctt gctccgctgg gattccgcac tcgccctgtg atcggtggcg
      841 atggccacat agacggcggg caacacgaac agcgtgaaca gggtcccgat cgacatgcct
      901 gcgacgacga caagcccgat cgagaaccgg ctcgccgcgc ctgcgcctga tgccgtcaag
      961 agcggcagca ggcccgtgac catcgccgcc gtcgtcatca ggatcggacg gagccggacc
     1021 cgcgccgcca tctcgatcgc cgagcgtttg tcgagacctt cattgagctg aagctcattg
     1081 gcgaactcga ccatcagaat gccgtgcttg ctgatcagcc cgaccagggt gagcaacccg
     1141 acctgggtat agatgttcat cgtggcggcg ccgaagaaca gcggaatcaa ggcaccgacg
     1201 atcgccatcg gcacgctgat catgatcacc aggggatcgc gcaggctttc gaattgcgcc
     1261 gccagcacca ggaagatgat gatcagcgca aaggcgaagg taatcgcgag ctggttgcct
     1321 tcctgcacat attgccggga atccgcgagg tagtcatggc tgtatccggc cggcaggttc
     1381 ttggcctggc tctcgaggaa gtccaccgcc tgcccaaccg tcacgccagg catcggcacc
     1441 gcctggaagg tcgcggagtt caactggttg tagtgggtca acgcattcgg atcggtcgcg
     1501 gtctcgatcg acacgaccgt cgagagcggt acctgctggc ccgtcgccgt cggcacgtaa
     1561 tatccgccga gcgcctcggg cgacaaccgt ctgtcgcgcg gcacctgggg aatgacctgg
     1621 tacgatcgtc cctcgaggtt gaaccggttc acatagtttc cgccgagcag cgtcgccagc
     1681 gtattgccga tgctcgccat ggtgatgccg aggtcgctcg ccttcgagcg gtcgatcttc
     1741 acccgcacca ccggctggtt gaaatcgaga tcgctgtccg agacgatgaa gagcccgctc
     1801 ttgcgcgcgg cggccttcag cttcagcatt tcctcataga cggactgaaa tccattggtc
     1861 gaattgatca ccatctgcac cggcaatcca ccgggaccgc cgggcagcgg cggcagattg
     1921 aacgcaaatg cattgacgcc ttcgagcttg ctcagctgtg cctgcaccag tggcttcagc
     1981 ttgatcgacg aacgctcccg ttcatcccat ggcttgagca gcatgccggc gattccgttt
     2041 tgtggaccat tgatgccatt gagagcaaaa cggagatcgg tttcgggaaa gccggcaaac
     2101 accttgtcga gtttctcacc gtagaaatcc agataatcga tgttggcgta tttgggcccc
     2161 ttgatcacgg agaacacgat gccctgatcc tcctccggag ccagctcctt cgaggtgtgc
     2221 atgtagagga agccgacgag cccgaggatc gttagcgcga acaagcccgt gatcggacga
     2281 tagtcgagcg aacgatcgag ctgccggcca taccagcgcg tcatcgaacc gaaaaccttg
     2341 ttgacgatct tcgcaaaccg tccctcttcc gcgctcttga ggaacaccga gcacatcatc
     2401 ggcgaaagcg tcagcgcgat cacgccggag acgatcaccg agccggccag cgtgaacgcg
     2461 aattcgcgaa acagcgaacc ggtgaggccg ccgagaaagc cgatcggggc atacaccgcg
     2521 gccagcgtga tggtcatgga gacgacggga ccgacgatct cgcgcgcgcc ctgcagggag
     2581 gcctgcacgg gaggtttgcc ttcctccaga tgccgatgga tgttctccac caccacgatg
     2641 gcgtcgtcga cgacgaggcc gatcgccagc accatcgcca gcaacgtcag caggttgaaa
     2701 ctgaatcccg cgatcagcat cagactgcag acgccgatca gcgacagcgg aatggtcacc
     2761 accgggatga tcaccgaacg gaacgatgcg aggaacagga agatcaccac caccacgatg
     2821 atgaccgcct cgcccagggt tttttcgacc tcgtcgatcg aggattgaat gaacttggtc
     2881 gaatcgtagg ccactttcat tttcatcgac ggcggcagat tgcgttcaag ttccggaaac
     2941 agcccgcgga cgcccttgac cagcgtcagc gggttgccct gcggcgtcgc ctgcacgccg
     3001 atgaagatcg cgtgctcgcc attgaaggcg acgctggcgt cggtgctctg cgcggccagt
     3061 tcgacggtcg cgatgtcctc gatccgcaca aagccgccgt ccttggattt gacgatcatg
     3121 cgcttgaact gatcgaggtt cttcagatcg gtgttggtcg atacgttgga gaccgtgagg
     3181 taacccttgg actgaccggc cgcggcctga aagttgttag ccctgatcgc cgcggagacg
     3241 tcgtccggcg aaacgttgcg gcccgccatg cggacgggat cgagccacag ccgcatcgcg
     3301 aaagtctggc cgcccagaat atcggcggat gcaacgccat cgacggtcga aagcaccggc
     3361 tgaaccaccc gcgtcaggta gtccgagatc gccgatcccg acagcacttc agaggagaag
     3421 ccgagataca tcaccgccgt ggtctggccg gtggtcttgg tcacgatcgg atcgttcgat
     3481 tccttcggaa tcagatattt gaccgagttc accttggcga ggacttcggt caacgcctgg
     3541 ttgggatcga aattcagctt gatgtagacc tggatggtgc tggtcccttg caccgaggac
     3601 gaggtcatat agtcgacgcc ttcggcggac gcgacggcct gctcgatcgg cgtcgtgatg
     3661 aatccctgaa tcagatcggc cgacgcaccg ggatagacgg tggtgacgtt gacgaccgta
     3721 ttcgacagtt tcggatattg ccggatcggc aatgtggtcg cggcccgaaa gccgatcaga
     3781 agaatcagca gactgacgac cacggacagc acgggtcgct tgatgaaaat atcggtccag
     3841 accatcgcta tgcgctccgt tagatcatga tccgatcaga ttgaatcgga tcatgatctc
     3901 atctgtttgt ttgagcatga tctccgagca aacgcttcgc gtttgtcccg agggaaaacc
     3961 ggtttccagt tttccgggtc atgctttagt aacgcggcgg attcgccgga atcggcggag
     4021 ctgcgtcggt tgaaatcgcc accgccgctc ccgattgcag cttgagctgg cccacggcga
     4081 ccacgcgatc gccgggtttc aacccgttga ggatttcggc tcgtccgccg atccggtcgc
     4141 cggtccgcac gaaggtccga acggcggtca gactggtctt gccatcatcc gccttcttct
     4201 cggtgagcag gaaaaccgaa tctccgtaga gcgtgtaatc gaccgccgtc tcgggaaccg
     4261 tgacgaccgc cggcttgtcg ggaagtacca ccgtcgtcgt tgcgaacatg cccggcttca
     4321 agatgtgatc gggattgtcg agcgtggcct gaacgcggat gttgcgggta tcggtggcga
     4381 tctggggttc gatcgcagtg atctttccct caaaggtacg gccgggataa gcgtcgacca
     4441 gaatccgcac ggtctgtccg accttgagtg ccgcgctgtc cttttcagtc accgtgaaat
     4501 tggcgtacag cgtcgacaaa tcggtcagcg tcacgatctg ggtgcccgcg gtgaggaact
     4561 ggccgacctc gacgtggcga acgccgagtt caccgtcgaa cggcgcgcgc accagcttct
     4621 gcgaaatgat cgcctcgctc ttggcgatgc ccgcattggc ctggtcatac gccgcctgcg
     4681 ccgaatcgac cgtggcctgc gggccgaact gacgcgaggc aagctgcttg gcgcgatcga
     4741 gcgatagctg cgccaccgtc gcctgcgcct tgaagttcgc aaggtcggcc tggtcgggag
     4801 cgtcgaacaa ttgcaccagc ggatttccgg ccttcaccgt cgcgccgggg gtgaacatga
     4861 tgtcggtgat gcggccgctg acgtcggacg tgacatcgac ctgatgcacg gcggcgagac
     4921 cgcccaccgc cgtcagcagg ttcggaatca cttcggattt cgcctccgcg atgctgacgc
     4981 tcgacggcgg cggcttgttg ttggcgaaga actgcgcaat catctggccg cggaagtaat
     5041 tgaaccagac gagaccgccc accaagagcg ccagcagcag cccgacgatg atgaaccagc
     5101 gcaccatccg gatcggacgc gcgcgcggtt tgtccgtgat cgccttgccc gagatattgg
     5161 cttcggtcac aatgttcatg tcatgcacct tctgcagtta ccggctgctg ccccggatcc
     5221 ggcgacaatt cgcgatccag atgagagacg acagcggttt cgttgagtcc aattccgcgg
     5281 agaatgaatt cgcaaatctg ccgctcgaaa tcggcagcgt tgccgtaggg gaggcatgga
     5341 accgagggca gccgcgccag cgtcgccatc agcacggtat gatgcgcaaa ccagaacagg
     5401 ttgagcggat cgcttccgat ccgtgacgca tcccctgccg ccacggcccg ctccagtgaa
     5461 gtggtgaaca caggtccgat caaattcccg atcttctcgt aaatgagccg ggcgaattct
     5521 ccgtcgtcga ggtggctggt gatcatcagc ctgagacgct gggcctcctg ctgatcggga
     5581 gcatcggcga cctctcggaa atggcggacc atttcgcgaa tcaggatcgc aagcgtttct
     5641 gtcgacggct ccaaccccag caaccggtgc aacgccggat ccgcctcgca ctcttccgca
     5701 aggatctccg catacaacgc tgatttcgtt ggaaaatgct tgaacagcaa tccctccgaa
     5761 attgaggcag cggcggcgac gcttttggtc gtggttcccg cgaagccgtg gcgggcaaaa
     5821 caccgcttgg cggcgctcag gatcaattgc cgccgcaagt cgccggtcat gcgcagggtg
     5881 ctcatgctcg tgagtaagca ctcaccttag caaatgtcaa gaatctgctg cagtgcacgc
     5941 gcggtccaga gccttttccg cttcgataga atcgaaacag ggctctagat tcgtgattcg
     6001 acacgttttc ttgacgcgaa ccggtctccg cgcccggatc aagtccgagg gcatcgcttg
     6061 aaaacgcttc agatcggacc aaatccgagc gcgccgcgga tgcgcgtcac ggtgaattga
     6121 ccgtcccggc tggaaagcac gcgctccaga ttagcgctgt cgatcttgat actggcaaag
     6181 cgaggcccgc tcgagacgtc ctgcatcgac gacgcaaagg ttccgatatc agccatggtg
     6241 gcgatcgcgc gggcgtccga gatcccacag gaccgggcga cgccgacgaa gtcgacggtt
     6301 gacgcggtgt ggctcgcctg cccgccggtc tcgccataga cctcgttatc cagcaccacg
     6361 atggtaagat ttgcaggctt ctgcagacta acggtcgcaa gactgcccat ccccatcagc
     6421 atctcgccgt cgcccgtaat caccaccacc ggcagtttgg gttgcgccag cgccatgccc
     6481 agcccgatca tgaccgcgcc ccccatggca ccccaaagat aaaagttgcg gtcatgatcg
     6541 ccggcggcgg cgatgtcgta ggtcgatgcg cccaatccgc cgaccacgat ggcgtcgttg
     6601 cgctccgcca gcaggtgaga gacgacgctg cgccgttcaa ggagatttgc ttggctcatt
     6661 tggtgaaaac ctttgccccg atcaggcgct gcgacaacag aaccgcggtc ggcgtgcagg
     6721 cattgtaggc ctgccccgcc gccgcctcga cgacctcgcg cacctcttcg gcgtgagagg
     6781 cccgcaacac cttgattccc gacagttcaa acacggccgc cgtgctcgac cccatcggca
     6841 cctgccaggg attgaattcg ccccattcgc cgcgcatcgt caccagcgtc aggaacggaa
     6901 agcggaaaat ctgggtcagc gacagcatgt tcacgcaatt gccgacgccg ctcgactgca
     6961 tcagcaacac gccgcgctgg ccaccggccc aggcgccggc caacagcgcc acgccttcct
     7021 cttccgtcgt cagcgggacc gcgcgcatcg aggatgcgcc cagcacgcgt tcgatcagct
     7081 ctgaatgccc ggcatccggc acgtaaggca cctgacggac atcgaagcgc tgcaaaatcg
     7141 cgaatatatc ctccggccag gaggcagcag tttcgggagg cgcgtccgtg cgagcgtgca
     7201 ttttgatttc cagtcggtcg gcgaggctgt attccggggc gggacaatag agccggcagc
     7261 gtttttcgaa agagcgaaaa cggcatatcg gtctctacct gtgagtatgg cagcatgaat
     7321 gcgaatgcgc gagagctggc ggccaatttc gacatcgaga agctgacggg cgaattctat
     7381 gccgacccct acccgaccta tcgtgccttg cgggagcatg agcccgtcaa gcttttgccc
     7441 aacggctccc gctttctgac gcgctatgac gacctcgtca gcgcctacaa gaacacaaaa
     7501 cttttctcgt ccgacaagag aaaagagttt ttgccgaaat atggcgattc cctgctttac
     7561 gagcaccaca ccaccagcct cgtcttcaac gatccacccg cgcacacccg cgtgcggcgg
     7621 ctgatcatgg gcgcgctgtc gccgcgcgcg attgccgaga tggagcccga tctgatcgcg
     7681 ctggtggacc ggctgctcga caccatcgcc gccaaaggaa gctgtgaact gatcggagat
     7741 tttgcatccg cgatcccggt tcaggtgatc ggcaatctgc tcgatgttcc catggaggag
     7801 cgcgaaccgt tacgcgactg gtcgctggct atactgggcg cgcttgaacc tgtgatcggt
     7861 cctgaagtgt ttgcccgtgg caacaaagcg gtgaaggatt ttcttagcta cctggaaacc
     7921 ctggttgcgc ggcgccgaac aaaacctggc aatcccgaac gcgacgtact gacgcgcctc
     7981 attcagggcg aggacaatgg cgagcgtctg accgaaacgg agttgctgca caactgcatc
     8041 ttcctgctca acgccggcca tgagaccacc accaacctga tcggcaatgg gctggtggcg
     8101 ttatcggaaa atcccgccga gaaaaaccgg ctgatcgaaa atcccggttc gatcaagacc
     8161 gcggtcgagg aaattctgcg atttgaaagc tcgaaccagc tcggcaaccg catgacgacg
     8221 gcgcaagtcg aactcagcgg catcacgctg gcagccggca cgccggtgac cttgtgcatt
     8281 ggtgctgcca accgcgatcc cgcacaattt gccaaaccgg aacatctcga catcggccgc
     8341 agccccaacc ggcatctcgc cttcggcacc ggcgcgcatc aatgcgccgg gatggcgctg
     8401 gcgcggctcg aaggcgccat tgcgatgtcg cgattcctgg c
//
LOCUS       DASWGM010000129         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_146509,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000129 DASWGM010000000
VERSION     DASWGM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146509"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGA15_04015"
     CDS             <1..204
                     /locus_tag="VGA15_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586892.1"
                     /translation="KKGRIHAAFSALETLPGAFGSATLSSLAACFSRAQINPLDKYCL
                     AVSSIVQHVVPAKAGTYNHQRSN"
     gene            complement(284..991)
                     /locus_tag="VGA15_04020"
     CDS             complement(284..991)
                     /locus_tag="VGA15_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9586893.1"
                     /translation="MAKAKRILKWQRDPEGMRLRILEAAKQEFAAHGLAGARVDRIAA
                     KAGANKRMLYYHVGKKDVLYLTVLEGAYEKIRVEERGLDLEHLDPPKAIERLIGFTWN
                     YFIRNPEFLALLNTENLARARHLKRSTKVKSMHSPFVEMIRTVVTRGVQSGDFPVAVD
                     PVQLYISIAALSFFYLSNSATLSVIFGRDLLKKEAKDERLEHMVALVLAALTGKSAAA
                     FAKNKTPKLRTPAHHTV"
     gene            complement(1082..1927)
                     /locus_tag="VGA15_04025"
     CDS             complement(1082..1927)
                     /locus_tag="VGA15_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9586894.1"
                     /translation="MITGLDHIVVLLGDIKAGAKAYELLLGRAPSWRSQSDGAETVLF
                     TLDNMTLELMAPAGSTPTADRIRSVIKIWGEGLASICFRVGDIAKMHRRLDRLALKPD
                     PVAEVESRDTDSGGTLSWKRTRAATDTTRGVRMFFLELAQERPHSAATAAAPILGLDH
                     VVVSTEDPERAAALYGARLGLDMALDRSHQDWGQLMFFRCGDLIVEVVRRPVAGADQT
                     HDKLWGLSWRVADIDATRARLAAAGLDVSEVRIGRKPGTRVVSVRDGTCGIHTLLVER
                     TARSA"
     gene            complement(2004..>2719)
                     /locus_tag="VGA15_04030"
     CDS             complement(2004..>2719)
                     /locus_tag="VGA15_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEX9586895.1"
                     /translation="GTLGRLEATGRGVAHLAKRVMKELAIDLNNGTAVIQGFGNVGSY
                     AARELHQFGLKVIAVSDHTGALYDATGLDIPALMQHSGRHGSIAGFSNELTFDPEQIL
                     TLPCDVLVPAAMERVIDAHVAENLKCRVLAEGANGPTTPDADLILEKRQDEIFLIPDI
                     LCNSGGVVVSYFEWVQDLQQLFWEEEEVMRREYQILDRAFDHMVTRSKADRIPHRTAA
                     MAIGVEKVRAAKTTRGLFP"
BASE COUNT          501 a          878 c          815 g          525 t
ORIGIN      
        1 aaaaagggac gaatccacgc cgcgttcagc gctttggaaa cgcttccggg ggctttcggt
       61 tcggccacgc tttcctccct cgccgcctgt ttttctcggg cacaaattaa cccgttggat
      121 aaatactgtc tagcggtttc cagcatcgtt caacacgtcg tcccggcgaa ggccgggacc
      181 tataaccacc agcgttcaaa ttaggacaga aggcctcggc cgccctgccc caaacgatag
      241 gacacggagt atgggtcccg gcccttcgcc gggacgacga gaatcaaacg gtatgatgtg
      301 ccggagtgcg cagtttcggc gtcttgttct tggcaaaggc ggcggcggat tttccggtca
      361 gggccgccag caccaatgcc accatatgct caagccgctc gtccttggct tccttcttca
      421 ggaggtcgcg gccgaaaatc acgctcaagg tggcgctgtt cgacagatag aaaaagctca
      481 aggccgcgat cgaaatgtag agctgcaccg gatcgaccgc caccgggaag tcgccgcttt
      541 gcacgccgcg cgtcaccacg gtgcggatca tctcgacgaa cggcgaatgc atcgatttga
      601 ccttggtcga gcgcttcaga tggcgcgccc gcgccaggtt ttcggtgttc aatagcgcca
      661 gaaattccgg attgcggatg aagtagttcc aggtgaatcc gatcaggcgc tcgatcgcct
      721 ttggcgggtc gaggtgttcg agatcgagcc cccgttcctc cacccttatc ttctcatagg
      781 cgccttcgag caccgtgagg tagagaacgt ctttcttgcc gacgtgataa tacagcatgc
      841 gcttgttggc gcccgccttg gccgcgatcc ggtcaacgcg cgcgccggcg aggccgtgcg
      901 cggcgaattc ctgcttggcg gcctcaagaa tgcgaagccg catgccctcg ggatcgcgct
      961 gccattttaa aattcgcttt gccttggcca aaaaactgcc gcctgataat gttgtgagat
     1021 cggatgtagc acggacaatc gggcttaaga aagaatgggc caccctctca gaggggaaga
     1081 gtcaagccga tcgcgccgtc cgctccacca gcagggtgtg gataccgcag gtaccgtcgc
     1141 gcacgctcac gaccctggtg ccaggcttgc ggccgatgcg cacctccgat acgtccaggc
     1201 ccgctgccgc cagccgcgcc cgcgtcgcgt cgatgtccgc gacgcgccag ctcaatcccc
     1261 ataacttgtc gtgggtctga tccgcgccgg cgaccggccg gcgcaccacc tcgacgatca
     1321 gatcgccgca tcggaagaac atcagttggc cccagtcctg gtgcgagcgg tcgagcgcca
     1381 tgtcgagccc gagccgcgcg ccatagagcg ccgcggcgcg ctccgggtcc tcggtcgata
     1441 ccacgacgtg atcgaggccc aggattggcg cggccgcggt cgcggccgaa tgcggccgct
     1501 cttgcgcgag ttcgaggaaa aacatccgca cgccccgcgt cgtgtcggtc gccgcgcgcg
     1561 tgcgtttcca gctcaaggtc ccgcccgaat cggtgtcgcg gctttcgacc tcggccaccg
     1621 gatccggctt cagcgccagg cgatcgagcc tgcggtgcat tttcgcgatg tcgccgacgc
     1681 ggaagcaaat gctggcgagc ccctcgcccc agattttgat cacgctgcgg attcggtcgg
     1741 ccgtcggtgt gctgcctgcc ggcgccatca gctcgagcgt catgttgtca agtgtaaaca
     1801 gcacggtctc ggcgccgtcg ctctggctcc gccacgacgg cgcccgcccg agcagcaatt
     1861 cgtaggcttt agcgccggcc ttgatatccc ccagcagcac gacgatgtgg tccaggccgg
     1921 taatcatcgg ggcaatccct cgatcaagaa atgacgcttt ggcttcacaa gcgtcgccgc
     1981 gagatttatg gaaacaggcc tctttatgga aacaagcctc gcgtggtctt cgcggcgcgg
     2041 accttttcca cgccgatcgc catggcggcg gtacgatgtg gaatcctgtc ggccttcgat
     2101 cgcgtcacca tgtggtcgaa ggcgcgatcg aggatctgat attcgcgccg catcacttcc
     2161 tcttcttccc agaacagctg ctgcagatcc tgcacccact cgaaatagct gaccacgacg
     2221 ccgccggaat tgcagaggat atccggtatc aggaaaattt catcctgacg tttttccagt
     2281 atcagatcgg cgtccggcgt ggtcggacca tttgcgccct ccgccagcac gcgacatttg
     2341 aggttttccg cgacatgcgc gtcgatcacg cgctccatcg ccgccggcac cagcacgtcg
     2401 cagggcagcg tgagaatttg ctcagggtcg aacgtaagct cgttggaaaa accggcgata
     2461 ctgccgtgcc ggccggaatg ctgcatcaac gcggggatat cgaggccggt cgcgtcgtaa
     2521 agcgcaccgg tgtgatcgct gaccgcgatc accttgaggc cgaactgatg caattcgcgc
     2581 gcggcatagg agccgacatt gccaaaaccc tggatcaccg cggtgccatt gttcaggtcg
     2641 atcgcgagct ccttcatcac ccgcttggcg agatgcgcga ccccgcgccc ggtggcttcg
     2701 agccggccca gtgtgccgc
//
LOCUS       DASWGM010000130         5061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1117399,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000130 DASWGM010000000
VERSION     DASWGM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117399"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VGA15_04035"
     CDS             complement(<1..930)
                     /locus_tag="VGA15_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX9586896.1"
                     /translation="MQNLRVAVDVGGTFTDICIMDEATGVIRIEKTSSTRDPIDGIMD
                     GVSKAGIDLSKVALFSHGTTVATNALITRRLPRAAVVTTNGFRDVIEIRRANKEDLWD
                     TYKDVVKPYVPRRDRLTVPERVDASGRVIEPLDVAAARQVARILKRRGIAAVAVCFMN
                     AYLNGENERAMRDILLEEMPNVPVSISSQVLPEIFEHERFSTTVVNAILSPVVVNYTS
                     RLGERLASEGYTRDLLLLHTGGGVMTPASVKDFAARLAGSGIAAGAIASRYIAGLCGF
                     PNSIGLDMGGTSTDVSLAYEGQSRITKDWYIEFG"
     gene            complement(1384..2343)
                     /locus_tag="VGA15_04040"
     CDS             complement(1384..2343)
                     /locus_tag="VGA15_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9586897.1"
                     /translation="MQTTYATTDIPPHSRRQFWQEIVSKTYFPLDLSFAGGSDFRASL
                     GAWSLGPVSVSRNVSDGLLYKRHERHLLSEREECYLITVPELAEIRFEQDGKDVRCRP
                     GAFLIERSHLPYEFSHRDPAALWVLKIPSAVLRARISRPERLATLQIDASRSVGALFV
                     DTLRLTGARIEEIDGPARALAGKHLIDLLAMAIESDERVLVGHSSSVRNAHLHRCEHF
                     IRARLDDTRLTPQMVADGSGISLRYLHQIFEREGITVCAYIRNQRLLMCDAMLRDPNC
                     RKSISEIAYQWGFGDQAQFSRNYRSRFGCTPSDVRAASRAPNP"
     gene            2582..2689
                     /locus_tag="VGA15_04045"
     CDS             2582..2689
                     /locus_tag="VGA15_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586898.1"
                     /translation="MKSLTPVVAEQAGVLFQVENEDAAMAGQPLFDLEV"
     gene            complement(3275..3886)
                     /locus_tag="VGA15_04050"
                     /pseudo
     CDS             complement(3275..3886)
                     /locus_tag="VGA15_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(3961..>5061)
                     /locus_tag="VGA15_04055"
     CDS             complement(3961..>5061)
                     /locus_tag="VGA15_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEX9586899.1"
                     /translation="SGRREPPEGMGAGLVYSRFNHPNSEIVEDRLSVYERTEKCALFS
                     SGMAAIATTILAFARPGDVILHSQPLYGGTETLFAKTLAGLSIDAVGFADGIDETAVG
                     LAAEDAMRKGRVAMIFIETPANPTNGLVDVAMVRRVAGMIGQAQGHTPIVACDNTLLG
                     PVFQRPIEHGADLSLYSLTKYIGGHSDLIAGAALGSKALMKDVKALRGAIGTQLDPHS
                     CWMISRSLETLSIRMEKADRNARLVADYLRDHAKVAKVHYLAHHEEASPAGRLFARQC
                     TGAGSTFAFDIVGGQAAAFKFLNALQIFKLAVSLGGTESLASLPASMTHSGVPADIRK
                     KIGILDSTIRLSIGIEHPSDLIADIAQAFNEA"
BASE COUNT          964 a         1521 c         1642 g          934 t
ORIGIN      
        1 gccgaattcg atgtaccagt ccttggtgat gcgcgactgg ccctcgtagg caagcgagac
       61 atcggtcgat gtgccaccca tgtcgagacc gatcgaattg ggaaagccgc acaggccggc
      121 gatgtaacgg ctggcgatgg caccggcggc aataccggag ccggcgaggc gggcggcaaa
      181 atccttgacg ctcgccggcg tcattacgcc gccgccggtg tgcagcagca gcagatctcg
      241 ggtatacccc tcgctcgcaa gacgctcgcc aagccggctc gtgtagttga caacgaccgg
      301 gctcaggatc gcattgacga ccgtcgtgga gaagcgctcg tgctcgaaga tctcaggaag
      361 cacctgtgag gaaatcgaga ccgggacgtt cggcatctct tccagcagga tgtcgcgcat
      421 ggcgcgctcg ttctcgccgt tgagataggc attcatgaag cagaccgcga ccgcggcgat
      481 gccgcggcgc ttcaggatgc gcgcgacttg gcgcgccgcc gcaacgtcga gtggctcgat
      541 cactctgccg ctggcgtcta cgcgttcggg tacggtcaac cgatcgcgcc gcggcacata
      601 tggcttgacg acatccttgt aggtgtccca gaggtcttcc ttgttggcgc gccggatctc
      661 gatgacgtcg cgaaagccat tagtcgtcac cacggcggcg cggggcagac ggcgcgtgat
      721 cagtgcgttg gttgcgacag tggtcccgtg cgagaacagc gcaaccttgg agaggtcaat
      781 cccggccttt gaaacgccgt ccatgatccc atcgatggga tcgcgggtcg acgatgtctt
      841 ctcgatgcgg ataacgccgg ttgcctcgtc catgatacag atgtcggtga aggttccgcc
      901 gacatcgaca gccacgcgaa ggttctgcac gacacgactt cctttcgaac acgcgctcgc
      961 catgccggac gttggggcga gtgcgcgggc tcagccggat ctatgatccg gttgccgcga
     1021 accgcccgtt cgaccggcgg atttcggagg tatcgcgaag attatacgca gctcgtctgc
     1081 ggcacgcttg acgctgagcg caggaagatt tgtgccacag agcaccaaca gcccaatttc
     1141 tggacatggc tgacatttaa agtgcagtca ggcgcaggca tgaaggcctc gcgagggcgg
     1201 acggtgccgt tatccatccc ggcggaacgg aagccccggc cttggggcaa agtggcggtc
     1261 agggtcagac gtccggcggg ccggtgacga tccaggcacg gatatcctcg agtgtggcga
     1321 cgctgtggct tcctgacgaa gaagaatgga gaaccattac ggggaccacg agcggcagcc
     1381 catttatgga ttgggcgcgc gcgaagccgc gcgcacgtcg ctcggtgtgc agccgaaccg
     1441 gctgcgatag ttccggctaa actgcgcctg gtcgccgaac ccccattggt aggcgatttc
     1501 ggagatgctc ttccggcagt tcggatcgcg cagcatggca tcacacatca atagacgctg
     1561 gttgcggatg taggcgcaca ccgtgatgcc ttctcgctca aagatctggt gcagataccg
     1621 gagagagatg ccggaaccgt ctgctaccat ctgtggcgtg agccgtgtgt cgtcgaggcg
     1681 tgcccggatg aagtgttcgc agcgatggag gtgggcgttg cggaccgagg acgagtggcc
     1741 caccagcacg cgttcgtcgg attcgatcgc catcgccaag agatcgatga gatgcttgcc
     1801 cgctagcgcc cgggcggggc cgtcgatttc ctcgatgcga gcgccggtaa gccgcagcgt
     1861 gtcgacgaag agcgccccga cgctgcggct ggcatcgatc tgcagggtgg caaggcgttc
     1921 ggggcgggag atccgtgcgc gcaggactgc actggggatc ttcagcaccc acagcgcggc
     1981 cggatcacga tggctgaact cgtagggcaa atggctgcgc tcgatcagga aggcgccggg
     2041 acgacaccga acgtccttgc cgtcctgctc gaagcggatt tcggccagtt caggcacagt
     2101 gatcaggtaa cactcctccc gctcgctcag gagatggcgt tcgtgcctct tgtagagtag
     2161 gccgtccgag acattgcggg ataccgaaac ggggccgagc gaccaagcgc ccaggctggc
     2221 gcgaaaatca ctgcctccgg cgaaggaaag gtccaacgga aagtaggttt tggagacgat
     2281 ttcctgccag aattgccggc gactgtgtgg gggaatatcg gtggtcgcgt aagtcgtttg
     2341 catgtcacgc actccgaatt cagtcgacac agggtcggta cagcgccggc ctcccgcgct
     2401 gtccgtcatg gtccagcatc ctctgcaact ctcgtgcccg agcaatcgga ctcatgagtg
     2461 ctaagacgat ccaagcacca ctcccgggca cgttctaccg gtctcccgct cctggtgaga
     2521 tggccttcaa ggcccgggtg acccggtggg cgtcggcgac acggtcggcc tgattgaggt
     2581 catgaagtcc cttacgcccg tcgtcgccga acaagcaggt gtcctgttcc aagtcgagaa
     2641 cgaggacgcg gcgatggcgg ggcagccgct gttcgacctt gaggtctgag ccgcggcgat
     2701 ggcgatttcc aaggttttcg tcgaaccgtc gcgagatcgc ggtgcgtgtc atccacgcgg
     2761 cccgcgactg ggcatccgga ccgtgcaagc ggtgagcgcg gcagatggcg gcattcaggc
     2821 tgcgcgtcac gccgacgaga ccgtgccagt cgggcctgct catgcgagca agtcctgcct
     2881 caacaaagag gcgatggctg ggattggtcg ccgcggctgg atctgcctcg taccccaatc
     2941 ggtccgaaaa ttgggacgtg gagacatcgt cttcatcgac attaatgagc ccaggctgac
     3001 cagtcccatc attatgggct ttcgaaccag cgatacatcc gtgccactct ccaacctccg
     3061 ttcgctcgtg tcggaattcg acaattggat gaggccagtt actccgggtt gatcgggctt
     3121 gttcagcctc actggtcacg tttggcccgc gaacgctaac ctgctttcgc aaacatcgcg
     3181 accagcgtat ctcgcagcca gccgatgccc ctgtcgctcg cgaagctgga tgggtgtgga
     3241 ccgtgatctc gaccggcggc aggtcgaacg gaagggccag caaacgaaac gccttcgcac
     3301 tgttgaactg gcgaccgaca ctttcgggaa agatgaccgc gaggtcggta tcgcggacga
     3361 tgtctggcgc caccgtaaag tgcccgagcc gaacggcgat ttgccgtttg atgtcagttt
     3421 cggcgagcca ttgctcgatc atctgatggc cgggcgcact ggtgccggcg tggacgtaac
     3481 gcagattgac gaaacttgct ttggtgagct tggaccgtcc gatcggatga ccgctgcgca
     3541 ccatgcagac aaacttgttc tcgaataacg cgtggcttgt gcattcgccc tcgagattcc
     3601 tgagatagcc gatggcaagg tcgacttccc cggcgcgcat ggcggcggcg accgtgtcga
     3661 ccgccagcgg cacgacgtcc agccgcaccg acggcgcaac gtgaacgagc cgggccatca
     3721 ggcgcggcag gtaataaacc tctgacacgt ccgacatcgc cacgcgaaac acccgttgcg
     3781 tcaccttggg atcgaaggcc acgcgttcct ggattgcgcg attgatgatg ccgaaggcct
     3841 gatcgagcgg ctcgtgcaaa cgcaccgcgg cctccgtcgg cagcatccgg tttgcggcgc
     3901 gcacaaacaa cgggtcgtcg aaccgatccc gtgaaggtcg ccaccacgca aggagtgcag
     3961 ctatgcctcg ttgaaagcct gcgcgatgtc cgcgatcaga tccgacgggt gttcgatgcc
     4021 gatcgacagc cgaatcgtgg agtcgagaat gcctattttt ttgcggatgt cagccggaac
     4081 gccggagtgg gtcatgcttg cgggcaggct ggcgagcgac tcggtaccgc ccaggctcac
     4141 cgccagcttg aagatctgca gcgcgttcaa gaatttgaat gcggcggctt ggccaccgac
     4201 aatgtcgaac gcgaacgttg agcccgcgcc ggtgcattgc ctcgcgaaca ggcggccggc
     4261 gggggaggcc tcttcgtggt gagcaaggta atggaccttc gcaactttgg cgtggtcgcg
     4321 caggtaatcc gccacgagcc gcgcgtttcg gtcagctttt tccatgcgga tgctcagtgt
     4381 ttcgagcgac cggctgatca tccagcagga atgggggtcc agttgggtgc cgatggcgcc
     4441 tcgcaacgct ttgacgtctt tcatgagggc tttggagccg agcgcagcgc ccgcgatcag
     4501 gtcggaatga ccgccgatat atttggtcag cgagtacaac gaaagatccg cgccgtgttc
     4561 gatcggccgc tgaaacaccg gcccgagcag cgtattgtcg cacgcgacga tcggcgtgtg
     4621 cccctgggcc tggccgatca tcccggcgac gcggcggacc atcgcgacat cgactaggcc
     4681 gttggtggga tttgccgggg tttcgatgaa aatcatcgca acccggcctt tgcgcatggc
     4741 gtcctccgcc gccagcccga ctgccgtttc atcgatacca tcggcaaagc cgacggcatc
     4801 gatggagagg cccgcaagcg tcttcgcaaa cagagtttcc gtcccgccat agagcggttg
     4861 agagtgcaga atgacgtcgc caggccgggc gaacgcaagg atcgtggtcg caatcgccgc
     4921 catgccggac gagaacagcg cgcatttctc ggtgcgctcg tagacagaga gcctgtcctc
     4981 gacgatctcg ctgttgggat gattgaaccg cgaataaacc aggcccgcgc ccatcccctc
     5041 gggaggctcg cgccgacccg a
//
LOCUS       DASWGM010000131         3757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1948413,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000131 DASWGM010000000
VERSION     DASWGM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948413"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /locus_tag="VGA15_04060"
     CDS             <1..130
                     /locus_tag="VGA15_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9586900.1"
                     /translation="VFICKLRKKLANASEGRNFIETVWGRGYVLREPHEVEERIPA"
     gene            384..788
                     /locus_tag="VGA15_04065"
     CDS             384..788
                     /locus_tag="VGA15_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX9586901.1"
                     /translation="MILQSLAGLPAFLVYFCTALVAVVAYLFVYTRVTPHDEFQLIRD
                     NMPSAAIALGLSLLGFVLPVVSAIAHSANVVDCLIWSAIALIVQIAVYFIVKIPVPNL
                     SARIAAGELAPAIWLGLASLAAGALNAASMIY"
     gene            791..1201
                     /locus_tag="VGA15_04070"
     CDS             791..1201
                     /locus_tag="VGA15_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586902.1"
                     /translation="MAEKPDREFGTRRPVAIQSSAPVKRSNHVALLLMGTFAIGGGAY
                     ALMPSESCEPNRPGMAAPAGPQAGAECPPRGSSSGSSHGSSGGSSSRSNFVSGDSSSH
                     SSSGTSSDSGSSGVTRGGFGSFAHAFTSHFSGGG"
     gene            1711..2868
                     /locus_tag="VGA15_04075"
     CDS             1711..2868
                     /locus_tag="VGA15_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX9586903.1"
                     /translation="MQRITCPERSDWRTTAEETGFDFHTIDGERYWDERAYYAFSLDE
                     IEDQIEAPSGEIDAMCLELVSNAVDDEDYLRRLKIPETFWPLISESWHRDEGSLYGRL
                     DLSFDGRGPAKLLEYNADTPTSIFEAAVFQWTWLEQAIERRIIPGHADQFNSIHECLI
                     DAWKKVGAGRHVHLAGTTENAEDAGTLAYLEDTARQAGLATTLIDIEDIGLRDDGSFA
                     DLDDRPIELVFKLYPWEWMFHDAFGARLADAPTRWIEPPWKAILSNKGILPLLWEMFP
                     DHPNLLPAFFEDDPNAAELGASFVRKPLYSREGANVALISEGVTVVEQQGPYGAEGFI
                     RQAFAPLPNFSQQYPVLGSWLIDHTPCGLSIREDENPITGNTSRFLPHAIL"
     gene            complement(3000..>3757)
                     /locus_tag="VGA15_04080"
     CDS             complement(3000..>3757)
                     /locus_tag="VGA15_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX9586904.1"
                     /translation="AGIAELVAKMKGGSDALTAEVAEAMTTNETFFFRDKIPFDHLRE
                     TVLPELLQARANRRSLRIWCAASSSGQEPYSIAMCLKEFASTLTGWRVEILATDLSQG
                     VLDKSRAGIFSQFEVQRGLPIQMLVKHFTQVGELWQLNADIRAMVQHRQLNLMQDFSH
                     LGTFDVIFCRNVLIYFDQYTKHGIFDRLAKVIEPDGVLALGAAETVVGISDAFKPYPD
                     RRGLYRPNLARTSRAGVIAMMPQALRAVVSAAG"
BASE COUNT          702 a         1160 c         1123 g          772 t
ORIGIN      
        1 cgtcttcatc tgcaagctgc gcaagaagct cgccaatgct tccgaaggcc gcaacttcat
       61 cgaaaccgtg tggggccgcg gctatgtgct gcgcgagccg cacgaggtcg aagagcggat
      121 tcccgcctga tcacgagtcg accgggagcc cgccaggctc ctcctccccg actggacccc
      181 gccgcaaatg gcggggtttt tgttagtgga ttgagccgcc ccttcgcgtg cgccgacgga
      241 cggatcgtgc aaccctgcgg ttgcatgagc cctttcggtt ccgattcgat ccgaacctag
      301 gctctatctt cttgttttga cgtgttttct tcacgcgaac cggtatccac ttcgctcgaa
      361 aacacgctgg gggcgccttt tcaatgatcc tgcaatcact ggccggactg ccggcgttcc
      421 tggtctattt ctgtacggcc cttgtcgcag tcgtcgccta cctcttcgtc tatacccggg
      481 tcaccccgca cgatgaattc cagctgatcc gcgacaacat gccttcggcc gcgatcgcgc
      541 tcggcctcag cctgctcggt ttcgtgctgc cggtggtcag cgccatcgcc cactccgcga
      601 atgtcgtgga ttgcctgatc tggagcgcga tcgcgctcat cgtccagatc gccgtctatt
      661 ttatcgtcaa gatcccggtg ccgaacctgt cggcgcggat cgcggccggc gaactcgcgc
      721 ccgcgatctg gctcgggctt gcatcgctcg ccgccggcgc gctcaacgcc gcctcgatga
      781 tctattgatc atggcagaga aaccagaccg ggagttcggg acgcgaaggc cggtcgcgat
      841 ccaatcgtcc gcgccggtca agcgctccaa ccatgtggca ctgcttctga tgggcacttt
      901 tgccattggc ggtggcgcct atgcgctgat gccaagcgag agctgcgagc cgaaccggcc
      961 ggggatggca gcgccggcgg gaccgcaagc cggagccgaa tgccctcccc gcggatcgtc
     1021 atccggcagc agccacggct catcgggcgg atcgtcgtcg cgcagcaact tcgtcagcgg
     1081 cgattcatca agccattctt cgtcaggtac gtcatccgat tccggctcaa gcggtgtgac
     1141 gcgcggcgga ttcggctcgt ttgcgcacgc gttcacgtcg catttttccg gcggcggctg
     1201 acaccagacc cattggccgt cctccgcacg atcctgtgaa acgcttggct ttggcttgtc
     1261 tgccgatcgc aagtctgcct caatgccaag ccttgaatgg gccgagactc ttcgagtgtg
     1321 tgagcgcagg tgatcgcatg aaacgttccc gccgtgtcat cttgaccatg atgggtaccg
     1381 cgacggttgg cgcggtatcg atgggctttg tgccgcgtat cgattgcggt cccggacgcg
     1441 ttccatatcg aacgcgcggc ccggacgggc gcctcattca aagttgccca gcgacatatg
     1501 gcggcttcgg cggtgcactg catcgattcc acgggcacgg gcatggccat ggtggacacg
     1561 ggcatgggca tggcggaggc tagcgcttga tccggatgcg ccgagtaact cgggtttctg
     1621 tctgggcctg atctttgggc atacacttcg catttgtccg gagggaaaac cggtttccac
     1681 tttttccgga tcatgctcta acatcaaccc atgcagcgta ttacttgccc cgaacgcagc
     1741 gactggcgga cgaccgcgga agagaccggg tttgatttcc acaccatcga tggcgagcgc
     1801 tattgggacg agcgcgccta ttatgcgttc tcgctggatg agatcgaaga tcagatcgag
     1861 gcgccgtcag gcgagatcga cgccatgtgc ctcgaactcg tcagcaacgc ggtcgacgat
     1921 gaagattatt tgcgccgatt gaagataccg gaaacatttt ggccgctgat ttccgagagc
     1981 tggcaccgtg acgagggctc actctatggc cggcttgacc tcagtttcga cggacgcggc
     2041 cctgcgaaac tgctggaata caatgcggat acgccgacct cgatcttcga ggcggcggtg
     2101 tttcagtgga catggctgga gcaggcgatc gaacggcgca tcattcccgg acatgccgac
     2161 cagttcaatt cgatccatga gtgcctgatt gacgcgtgga aaaaagtcgg cgccggacgc
     2221 cacgttcatc tcgccggaac gaccgaaaac gccgaggatg ccggcacgct ggcctatctc
     2281 gaggacaccg cgcggcaggc cggtcttgcg accacattga tcgacatcga ggacatcgga
     2341 ttgcgcgacg acggcagctt cgccgatctt gacgatcgtc cgatcgagct ggtgttcaaa
     2401 ctgtatccct gggaatggat gtttcacgat gcgttcggcg caaggttggc cgatgcgccg
     2461 acgcgctgga tcgagccgcc atggaaagcc atcctttcca acaagggcat tcttcccctg
     2521 ctgtgggaga tgtttccgga tcatcccaat ctgttgccgg cttttttcga ggacgacccg
     2581 aacgcagccg agctcggcgc ctcctttgtg cgcaagccgc tctattcccg cgaaggggcc
     2641 aatgtcgcgc tgatcagcga gggcgttacc gtggtggaac agcaggggcc gtacggcgcc
     2701 gagggtttta ttcgccaggc cttcgcgccg ctgccgaatt tttcccagca atacccggtg
     2761 ctcggcagct ggctgatcga tcacacgccg tgtgggctat cgatccgcga ggacgagaat
     2821 ccgattaccg gcaatacgtc gcggttcttg ccgcacgcaa tcctctaagc cctgctccag
     2881 agcgttttcc agcgaaagcc tgccccggac ttgatccggg tggagaccgg ttcgcgccaa
     2941 gaaaacgcgt cgaatcaaga atccagagcc ccgtttcgat tctatcgaaa cggaaaaggc
     3001 tagcccgccg ccgaaacgac tgcccgcaac gcctgaggca tcatcgcgat gacgcctgcg
     3061 cgtgacgtcc gggcgaggtt cgggcggtac agcccgcgcc gatccggata gggcttgaag
     3121 gcgtcggata ttccgacaac ggtttcggct gcgcctagcg ccagcacgcc gtcgggttcg
     3181 atcaccttgg caaggcgatc gaagatgccg tgcttggtgt actgatcgaa atagatcagg
     3241 acattgcggc aaaagatcac gtcgaacgtt ccaagatgag aaaagtcctg catcaggttc
     3301 agttgccggt gctgcaccat ggcgcggata tcggcgttga gctgccacag ctccccgact
     3361 tgcgtgaaat gcttgaccag catttgaatg ggcaatccgc gctggacctc gaactggctg
     3421 aagatgccgg ccctggattt atccagcaca ccttgagaca gatcggtcgc aagaatttca
     3481 acccgccatc ccgtcagcgt cgacgcaaat tccttcaggc acatcgcgat cgaatacggc
     3541 tcctgcccgc tggaggaagc cgcacaccag atccgcaggg aacggcgatt ggcgcgcgcc
     3601 tgcagaagct cggggagcac cgtttcccgc agatgatcga acggaatctt gtcgcgaaaa
     3661 aagaaggttt cattggtggt cattgcctcg gccacctccg ccgtcagcgc gtcggatcca
     3721 cccttcattt tcgcaacgag ttcggctatt ccggcca
//
LOCUS       DASWGM010000132         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2229116,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000132 DASWGM010000000
VERSION     DASWGM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229116"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VGA15_04085"
     CDS             complement(<1..1113)
                     /locus_tag="VGA15_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586905.1"
                     /translation="MPQPMKIAAVAAAMFLAAAAPAHAADTATADDTARFLAGMPPSA
                     ASPLTPLMQDPSWQQHARFFDAAFGQLEQRQLSKIRGWADANLSAPRPTMFYMFSGPD
                     FLYADAFYSKATTYVLGALEPVGAVPDLTRLPRGGIASNLYDVERSLGSILSFSFFIT
                     KEMKTDLRAGQISGTLPLLYVFLARSGKTIRDVSLVALDDKGTAYFANENAGSNPTRG
                     VRIVFASSDGQEKTLYYFSTDLSNRGIKSSGFLQFCQTLAPAGSLIKSASYLLHSGNF
                     TTVRDFLLANSATIIQDDSGIPLANYDPRKWRFFPFGRYAGPIDKFPGAYQPKYAELF
                     RRAQPMDFGIGYRWRSFESNLLLSVQLPSDGSGSADA"
     gene            complement(1116..2489)
                     /locus_tag="VGA15_04090"
     CDS             complement(1116..2489)
                     /locus_tag="VGA15_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9586906.1"
                     /translation="MAASETGSAAWPGRFSGGGTTVSVIVATAIVVADMVGVGVFTSL
                     GFQVKDIPSGFSILLLWTIGGIVALCGVFSYSELGAMFPRSSGEYNFLGRAFHPAFGF
                     LAGWVSATVGFAAPVALAAMAFGEYGKAVLPGAPPLALAIGVVWLVSIVQLGGVKHSS
                     TFQLISTILKVVLIVAFLIAGFVIGTPQPVSFAPRASDLAHVASAPFAIGLVFVMYSF
                     SGWNAATYIIGEMHTPQQSLPRALLAGTLIVLVLYVALNAVFLYTTPIDRLSGQLDVA
                     RIAGSSIFGEIGGRIVGAMICIGLVSSISAMMWIGPRVMMTMGEDIPALRAFARKSSN
                     GAPAYAILFQLAVASLMLFTRSFEAVLDFIQFALLFCSFFTVLGVIKLRITQPDLPRP
                     YRAWGYPVTPVVFLLVTAFMMYYLLMERPVQSLLGALMMISGLLIYAIFRKRADRGPA
                     TLSASRE"
     gene            2793..4109
                     /locus_tag="VGA15_04095"
     CDS             2793..4109
                     /locus_tag="VGA15_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9586907.1"
                     /translation="MVGDNPKFQTSDRVGQVKPPGATQHARAQAAQHYRAPTRLRRIA
                     LLTALVPLSLALVQCGKAPNAEILAANSQDSGDSFDDRFPAPQFKDRFPNANESFVQW
                     QSSDALRKRTAQAQGAPYRVASLAPTVPFQLPPIREEQTTLVGLNSSAFPYLGNNPRT
                     DEPFLNVSKGDRRGHRGLGGKVFWQDETYNDNRVLMHVPENFDIRKPGVIVVFFHGNG
                     ATLERDVRDRQLVPQQISESGVNAVLLAPQLAVDAADSSAGKFWQPGGLKRFVAESAE
                     HLAALYGNPGTARAFANMPVVIVGYSGGFMPAAWSLEVGGLGNRVRGVFLLDAVYGEL
                     DKFASWIVNNRSGFFVSSYTHYTKRHDDELIKMLKEKGIAISEDMDGPLRPGSVVFVE
                     TQEGVTHRDYVTHAWTENPVREMLVKMAATPALTRMAASAPSSANR"
BASE COUNT         1016 a         1466 c         1457 g          843 t
ORIGIN      
        1 cgcgtcagcg ctccccgaac cgtccgaggg aagctggacc gacagcagca ggttcgattc
       61 aaacgatcgc caccgatagc ctatgccgaa atccatcggc tgagcccgcc tgaacagctc
      121 ggcatatttc ggctggtatg ctccggggaa tttgtcgatc ggaccggcgt agcggccgaa
      181 cggaaagaac cgccatttcc tcggatcgta attggcaagt ggaatgccgg aatcgtcctg
      241 gatgattgtg gcgctattgg cgagcagaaa atcgcgcacg gtggtgaaat tgccggaatg
      301 cagcagatag gacgcgctct tgatcaggct gcctgccggc gccagggttt ggcaaaactg
      361 caggaagcct gagctcttga tgcccctgtt ggaaagatcg gttgagaaat aatacagcgt
      421 cttttcctga ccatcgctgc tggcgaacac gatccgcacc ccgcgcgttg ggttgctgcc
      481 ggcattttca ttggcgaaat acgcggtccc cttgtcgtcg agcgcgacca gactgacgtc
      541 gcggatggtc tttcccgagc gcgccaggaa gacatacaaa agcggcaagg tgccgctgat
      601 ctgcccggcg cgcaggtcgg tcttcatctc tttggtgatg aagaagctga agctcaggat
      661 cgagcccagc gagcgctcca catcgtagag gttggacgcg ataccgccgc gcggcagcct
      721 ggtcagatcc ggcaccgccc cgaccggctc gagcgcaccg agcacgtagg tcgtcgcctt
      781 ggaatagaac gcgtccgcgt agagaaagtc cgggccgctg aacatgtaga acatggtcgg
      841 ccgcggcgcg gacagattgg cgtccgccca accgcggatt ttcgaaagct ggcgctgctc
      901 aagttgtccg aacgcggcat cgaagaatct ggcgtgctgc tgccacgaag ggtcttgcat
      961 cagcggcgtc agcggcgaag cggccgacgg cggcattcct gccaggaaac gcgcggtatc
     1021 gtccgcggtc gcggtgtcgg ccgcgtgcgc gggcgccgcg gcggcgagaa acatggccgc
     1081 agcgaccgcc gcaattttca tcggctgcgg catcgctatt cgcggcttgc ggatagggtc
     1141 gcgggacccc gatcggcccg cttgcggaag atggcgtaga tcaagaggcc cgatatcatc
     1201 atcaaagcgc ccagcaacga ctgcaccggc cgttccatca agagatagta catcataaag
     1261 gcggtcacga gcaggaaaac caccggggtt accgggtatc cccaggcgcg atagggccgc
     1321 ggcagatcag gctgcgtgat acgcagcttg atgacgccga gcacggtgaa gaacgagcag
     1381 aacaacagcg cgaactggat gaaatcgagc acggcctcga aactgcgggt gaacagcatc
     1441 aggctggcta ccgcgagctg gaacaggatg gcgtaggccg gcgccccgtt gctcgatttg
     1501 cgggcaaatg cccgcaaggc cggaatgtcc tcccccatgg tcatcatgac gcgcggaccg
     1561 atccacatca tcgcgctgat ggaagacacg agcccgatac agatcatggc gccgacgatg
     1621 cggccgccga tctcgccgaa aatgctgctg ccggcgatcc gcgcgacatc gagttgcccg
     1681 gacagtctgt cgatcggcgt ggtgtagagg aataccgcat tcagcgcgac atagagcacg
     1741 agcacgatca gcgtccccgc cagcagcgcg cgcggcaggc tctgctgcgg cgtgtgcatc
     1801 tcgccgatga tgtaggtcgc ggcattccat ccggagaacg aatacatcac gaacaccagt
     1861 ccgatcgcga acggggcgct ggcgacgtgt gcaagatcgg aagcccgcgg cgcgaacgac
     1921 accggttgcg gcgtgccgat gacaaatccc gcgatcagga aggctacgat cagcacgact
     1981 ttcaagatgg tcgagatcaa ttgaaaggtg ctcgagtgct tgacgccgcc gagttgaaca
     2041 atcgacacca gccagacgac gccgatcgca agcgcaagcg gtggcgcgcc cggaagcact
     2101 gccttgccgt attccccgaa agccatcgcc gccaatgcca ccggcgcggc gaagccgacc
     2161 gtcgccgaca cccagcccgc cagaaatccg aacgccggat gaaaggcgcg accgaggaaa
     2221 ttgtattccc cgctcgagcg cggaaacatg gcgccgagtt cactgtagga aaaaacgccg
     2281 cacaacgcga cgatgccccc gatcgtccat agcagcagga tcgaaaagcc cgaggggatg
     2341 tccttgacct ggaagcccag gctggtgaag acgccgacgc cgaccatatc ggccacgacg
     2401 atggcggtag cgacgatgac cgagacggtg gtcccacccc cagagaaccg gccgggccaa
     2461 gccgcgcttc ccgtttctga tgccgccatg ccgatccacg ccttacgcgt cacgcccgtg
     2521 attcttcatc gtgcaggctc gccccgttca attcaagcaa tgttaacaag gcttaaatac
     2581 aaacccgcgt tagggtatta ttgtacttca aaggcctctc cggtgttcta cggcattgcg
     2641 aaatcaaagt gaccggccca caatcgcgac acgatttcgt gttcgcgtgg gggcgttccg
     2701 ggagtgggga gtattcccgg atgcttaacg tcacctcaac gttagcgagc tgaaatacgc
     2761 cgatcgccgg agggtgttgg ggcaaggggt ggatggtcgg agacaatccg aaattccaga
     2821 cgtctgatcg cgtgggccag gtgaagccgc caggcgcgac gcagcatgcc cgcgcgcagg
     2881 ctgcgcagca ttatcgcgcc cctactcgac tgcgtcggat cgcccttctg acagcacttg
     2941 tcccgctatc gctcgcgctc gttcaatgcg gcaaggcgcc gaatgccgaa attctggccg
     3001 ccaactcgca agattcggga gattcgttcg atgatcggtt cccggcgccg caattcaagg
     3061 atcgcttccc gaacgccaat gagagcttcg tgcagtggca atcctccgac gcactccgca
     3121 aacgcacggc tcaggcccaa ggcgccccgt accgagtggc gtcgctcgcc ccgacggtgc
     3181 cgttccaact accgccgatc cgggaagaac agaccacgct ggtcggcctg aattcctcgg
     3241 cgtttcccta tctcggcaat aatccgcgca ccgacgagcc gttcctgaac gtttccaagg
     3301 gcgaccgccg cggccaccgc ggtctgggcg gcaaggtttt ttggcaggac gagacctaca
     3361 acgataaccg cgtcctcatg cacgtcccgg agaacttcga tatccgcaaa ccgggcgtga
     3421 tcgtcgtgtt cttccacggc aatggcgcga cgctggagcg cgacgtgcgc gaccggcaat
     3481 tggtgccgca acagatttcg gagtccggcg tcaatgccgt gctgctggca ccgcaactgg
     3541 cggtcgatgc cgccgattcc agcgccggca agttctggca accgggcggg ctcaagcgct
     3601 tcgtggcgga gtcggccgag cacctcgccg ctctctatgg caatccgggc accgccaggg
     3661 ctttcgccaa catgccggtc gtgatcgtcg gctacagcgg cggctttatg ccggcggcat
     3721 ggagtcttga agtgggcggc ctcggcaacc gcgtgcgcgg cgtgttcctg ctcgacgcgg
     3781 tttatggcga gctcgacaag ttcgcgtctt ggatcgtcaa caaccgatcc ggcttcttcg
     3841 tcagctcata tacgcattac accaagcggc acgatgacga actgataaaa atgctgaagg
     3901 aaaaaggcat cgccatatca gaggacatgg acgggccgtt gcggcccggc agcgtggtat
     3961 ttgtcgagac acaagagggc gtgacgcatc gcgattacgt cacccacgcc tggaccgaaa
     4021 atcccgtcag agaaatgctg gtcaagatgg cggcaacgcc tgccctgacg aggatggctg
     4081 cgagcgcccc gtcgtccgcg aatagatgat ccggtgcgcc ttcacgccgt cgcatcgccg
     4141 caacttccct gtcatatcca ccgcccaata aaagatgccg ttgaggacgc gccggtcatc
     4201 aacccgggga atgccacgcg gctggtttgg aagaacagcc tgatcgcagc ccattcaaag
     4261 tctgtgagtt gataccgtac gattcgcttg ccccccggtt ttggcgttcg aatcatgccc
     4321 aagccgatgc cttcaacggc aaacccggac aaccggcagt tccgagatta ccccctaacc
     4381 ggagatatcc cgggcatcac aaaccgacgc gaatgacccc agaacggacg tcggaagcgc
     4441 ggcgcggcga tcattctgag ttcgcccaat gttcaggttc tgtaactgtg gcgcgctcgg
     4501 taagggcctg gtttaaccct cagtggccta atttgaattt cgcagtggcc taatttgatt
     4561 ttggagactg gtctctttcg cagactgcgt gatgctggtc atgcagtcag ccacagttgt
     4621 gatcttcgga actgacgtga cctccggcac agatggctgg ctcgcactcg catacccctg
     4681 tcgccacggg ctgaggaata gtcctcactg aggaacacac aggagaatga catgaccggg
     4741 ttgaacaacg acatttgtga actgaacatc gatgaactcg ac
//
LOCUS       DASWGM010000133         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2505631,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000133 DASWGM010000000
VERSION     DASWGM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2505631"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VGA15_04100"
     CDS             <1..202
                     /locus_tag="VGA15_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX9586908.1"
                     /translation="KTGQLENKDDIKRRLEEASKFVSLDQLAVSPQCGFASTEEGNIL
                     TEEEQWAKLRLAVDVAKEVWGK"
     gene            226..1374
                     /locus_tag="VGA15_04105"
     CDS             226..1374
                     /locus_tag="VGA15_04105"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9586909.1"
                     /translation="MQDPDAIIVGAGPAGLACAVTMRAAGLDVVVVEKADTVGSVWRR
                     HYDRLHLHTDRKHSGLPGMVMPKTYPLYPTRGQLVEYLESYAARFDIRPAFNSTASCI
                     RRDGTQWRVELAQGSISAPVVVVATGIANAPYRPSWPGSELYQGPVVHSSEYCNPAPY
                     AGKRVLVVGFGNSGGEIALDLANAGVDVTLAVRGPVQILPRDLLGFPILAWAILYQRL
                     PARLVDLINAPILRLAIGRIEKLGLRRAAKGPRQMVEEDGRVPLIDVGTLDKIRDGSI
                     RIGGGIDRFTPDGVVLADGEWKKFDAIILATGFRPDLRQLIADVEGVFDRHGMPLVTG
                     RATSLPGLYFCGQITSPTGQLREIGLEAQRIADSARSYRATLHGFPAR"
     gene            complement(1353..>1819)
                     /locus_tag="VGA15_04110"
     CDS             complement(1353..>1819)
                     /locus_tag="VGA15_04110"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9586910.1"
                     /translation="PVDVRLQAGGNVTETMIDMDTILAGLDDQVRTNILIFDACRNNP
                     MARQTASAVPDRSIEAGSGLAAPTAPNSGAALGAGTLIAFATAPGQVALDGQGANSPF
                     SAALSRHIGAPGLEVQQMLTRVRAEVVAATKNKQVPWSNSSLLGEVYLAGKP"
BASE COUNT          320 a          529 c          607 g          363 t
ORIGIN      
        1 gaagactggc caactcgaga ataaggacga tatcaagcgg cggctggagg aggcgagcaa
       61 gttcgtgtcg ctggatcaac tcgcggtatc gccgcaatgc ggctttgcct cgaccgaaga
      121 gggcaatatc ctcaccgagg aagagcagtg ggcgaagctg aggctcgcgg tcgatgtcgc
      181 gaaagaggtg tgggggaagt aagtcattcg cttgcggagg gcgtattgca ggatcccgac
      241 gccatcatcg tcggtgccgg ccccgcgggg ctggcatgtg ccgtcacgat gcgcgcggcg
      301 gggcttgacg ttgttgtagt cgagaaggcg gataccgtcg gctcggtgtg gcggcgccat
      361 tacgatcgac tccatttaca caccgatcgc aaacattccg gcttgcccgg catggtcatg
      421 ccgaagacct atccgctgta tccaacgcgc gggcaattgg tcgagtatct cgaaagctat
      481 gccgcccgct ttgacattcg gcctgctttc aattcgacgg cgtcctgcat tcggcgcgac
      541 gggacgcagt ggcgcgtgga gctggctcaa ggctcgatct ctgctcccgt tgtggtggtt
      601 gcgaccggca tcgccaatgc gccctatcgt ccttcgtggc cgggttcgga gctttatcaa
      661 gggccggtcg tccacagcag cgaatattgc aatccggccc cctacgccgg aaagcgcgta
      721 ctggtcgtcg gatttggcaa ttcgggcggt gaaatcgcgc tggacctggc gaacgccggc
      781 gtcgatgtca cgctggcggt tcgaggaccg gttcagatct tgccccgcga tctcctgggc
      841 tttccgatcc tggcgtgggc cattttgtat cagcgtctgc cggcgcggtt ggtcgatttg
      901 atcaacgcgc cgatattgcg gctggccatc ggtcgaatag aaaagctggg attgcgccgc
      961 gcggccaagg gaccgcgcca gatggtcgag gaggatggac gcgtgccgtt gatcgatgtc
     1021 ggcacgctgg acaaaatcag ggacggctcc atcaggattg gcggcggcat cgatcgattt
     1081 acgcctgacg gcgttgtgct ggccgatggg gaatggaaaa aattcgacgc gatcattctt
     1141 gcgaccggct tccgtcccga cctgcgccaa ttgatagctg atgtggaagg cgtgttcgac
     1201 cggcatggaa tgccgctggt gacgggccgg gcgacgagcc tgcccgggct ttatttttgt
     1261 ggccagatca cgtccccgac gggccagctg cgggagatcg gccttgaagc gcagcgcatt
     1321 gccgacagtg ccagaagcta tcgggcgacg cttcatggct tccccgcaag atagacttcg
     1381 ccgagcaacg aagaattcga ccatggtacc tgcttgttct tggtcgcggc gacgacttcc
     1441 gcacgcacgc gcgtcagcat ctgctgcacc tcgagtcctg gcgcgccgat gtgacgcgac
     1501 agtgccgcgg agaacgggct gttggcgcct tggccgtcga gcgccacctg tccgggcgct
     1561 gtcgcgaatg caatcagcgt tccggcgccg agcgccgcgc cggaattcgg cgcggtcggc
     1621 gccgccagtc ccgatccggc ttcgatcgaa cggtccggta cggcggatgc ggtttgccgc
     1681 gccatcggat tattgcggca ggcgtcgaag atcaggatgt tggtgcgaac ctggtcatcg
     1741 aggccggcaa gtatagtatc catgtcgatc atcgtttcag tcacgttgcc gccggcctgt
     1801 aaccgcacat ccaccggca
//
LOCUS       DASWGM010000134         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2505662,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000134 DASWGM010000000
VERSION     DASWGM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2505662"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /gene="phnK"
                     /locus_tag="VGA15_04115"
     CDS             <1..259
                     /gene="phnK"
                     /locus_tag="VGA15_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HEX9586911.1"
                     /translation="FMDEPTGGLDVSVQARLLDLMRSLVSELGLSAIVVTHDLAVARL
                     LSHRVMVMKGGRVIETGLTDQVLDDPCEPYTQLLVSSILPA"
     gene            270..968
                     /gene="phnL"
                     /locus_tag="VGA15_04120"
     CDS             270..968
                     /gene="phnL"
                     /locus_tag="VGA15_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HEX9586912.1"
                     /translation="MTAMIDISDAEKTFTMHLQGGVELPVVRGVSFQVRAGECVVLSG
                     PSGAGKSSILKMIFGNYRCDGGRIGICHEGVQIDLAKAEPRQILSVRRATIGYVSQFL
                     RAVPRVAAIDVVAEPLIANGAARGPAREQAGSLLRALNIPQRLWPLPPATFSGGEQQR
                     VNIARGFISDLPILLLDEPTASLDAANRAVVVDLINEKKRAGVAMVAIVHDEEIRHRI
                     ADRIVDVTSFAAAV"
     gene            984..>2130
                     /locus_tag="VGA15_04125"
     CDS             984..>2130
                     /locus_tag="VGA15_04125"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEX9586913.1"
                     /translation="MTSTKDETVLGNARIVLADRVIERGWIAFADGRVAEVGEGNGPA
                     GSGDAEGDLIMPGLIELHTDHLEAHYVPRPKVFWDPIAAVVSYDGQLATSGITTVLDS
                     LRVWREDGAEDVDGRAGILAEAIASARDANLLRADHFLHLRCEIPMPSVVEEAKELVG
                     RPDVRLMSLMDHTPGQRQFRDEGKLRTYYRGKGAGMTDAELDMLFERRFAYQKAHAAT
                     NMREIVALAQQYEIPLASHDDTTDENVTDAIRDRVSVAEFPTTIEAARGLHRAGIGIL
                     MGAPNVVRGGSHSGNIAAVDLAREGLLDILSSDYIPSSLLMAALQLPQRVPAIDLASA
                     VGTVTKTPAEAVGLTDRGEIAPGKRADLIRVHIARDIPVVRSVWREGH"
BASE COUNT          371 a          634 c          719 g          406 t
ORIGIN      
        1 gttcatggat gagccgaccg gaggcctcga tgtgtcggtg caggcgcgcc tgctcgatct
       61 gatgcgaagc ctggtgagcg aattgggatt gtcggccatt gtggtcaccc atgatctcgc
      121 ggtggcgcgg ctgctgtcgc accgggtcat ggtaatgaag ggtggtcgcg tcatcgagac
      181 cggcttgacc gatcaggtgc tcgacgatcc ctgtgagcct tatacccagt tgctcgtctc
      241 ttcgattttg ccggcatgag ggttggccga tgactgcgat gatcgatatc agcgacgcgg
      301 agaaaacctt caccatgcat ttgcaaggcg gggtggagtt gccggtggtg cgtggcgtct
      361 cctttcaggt ccgggccggc gagtgcgtgg tgctgtccgg cccctcgggc gccggcaaat
      421 cctcgatcct gaaaatgatc ttcggcaatt accgctgcga cggcggccgt atcggtattt
      481 gtcacgaagg tgtgcagatc gacctcgcca aagccgagcc gcggcagatc ctcagcgtcc
      541 gccgcgccac catcggctat gtcagccagt tcctgcgtgc ggtgccgcgg gttgcggcga
      601 tcgacgtggt ggccgagccc ttgatcgcaa atggcgcggc acgggggcct gcgcgcgaac
      661 aggccgggag cctgttgcga gccctcaaca tcccccaacg tctgtggccg ctgccgcctg
      721 cgacattctc gggaggcgag cagcagcgcg tcaacatcgc gcgcggtttt atttccgatc
      781 tgccgatcct gctactcgac gagccgacgg cttcgctcga tgccgccaat cgcgccgtgg
      841 tggtggatct gatcaatgaa aagaagcgtg cgggcgttgc catggtcgcc atcgtccatg
      901 acgaggagat ccgccatcgg atcgccgacc ggatcgtcga cgtaacatcg tttgccgccg
      961 cggtatgaag gaagacgctt aagatgacct cgacaaagga cgagaccgtg cttggcaacg
     1021 ccaggattgt tctggcggac cgcgtgatcg agcgcggctg gatcgccttt gccgatggcc
     1081 gcgtcgccga agttggcgag ggtaacggac ctgcgggcag cggggacgcg gaaggcgacc
     1141 tgatcatgcc cggcctgatt gagttacata ccgatcatct cgaagcgcat tacgtgccgc
     1201 gcccaaaggt gttctgggac ccgatcgccg cggtcgtttc ctatgacggg caactggcga
     1261 catcgggcat caccacggtg ctggattcgc ttcgggtctg gcgcgaggac ggcgcggaag
     1321 atgtcgacgg gcgggcaggc atactggccg aggcgatcgc atcggcgcgc gacgccaatc
     1381 tgctgcgtgc cgatcatttc ctgcatctgc gctgcgaaat tccgatgccg agcgtggtcg
     1441 aggaagccaa ggaactggtt gggcggccgg atgtgcggtt gatgtcgctg atggaccaca
     1501 cccccggaca gcgccagttt cgcgacgaag gtaagctgcg cacctattat cgcggcaagg
     1561 gcgccggcat gacggacgcc gagctcgata tgctgttcga acggcgcttt gcgtatcaga
     1621 aggcgcatgc ggcgaccaat atgcgcgaga tcgtggcgct ggcgcagcaa tatgaaatcc
     1681 cgctcgccag tcacgacgac acaacagacg aaaacgtcac ggacgccatt cgcgaccggg
     1741 tttcggtggc ggaatttccg accacgatcg aggccgcgcg gggattgcac cgggccggca
     1801 tcggcatcct gatgggagcg ccgaatgtgg tccgcggcgg ttcgcattcc ggcaacatcg
     1861 ccgcggtcga tctcgcccgc gaggggctgt tggatatact gtcgtccgac tacattccct
     1921 cgagcttgct gatggcggcg ctgcaattgc cgcagcgggt gccggccatc gatctcgcat
     1981 ctgccgtcgg tacggtcacc aagacgccgg ccgaagcggt tggcttgacc gatcgcggcg
     2041 agatcgctcc gggcaagcgc gccgatctga ttcgcgtgca tattgcccgc gacattccgg
     2101 tggtgcgcag cgtctggcgt gaaggacatc
//
LOCUS       DASWGM010000135         5506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2643458,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000135 DASWGM010000000
VERSION     DASWGM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2643458"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VGA15_04130"
     CDS             <1..123
                     /locus_tag="VGA15_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX9586914.1"
                     /translation="RFQINAQNCVHCKTCDVKDPNGNITWVPPEGGGGPNYEAM"
     gene            365..2149
                     /locus_tag="VGA15_04135"
     CDS             365..2149
                     /locus_tag="VGA15_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9586915.1"
                     /translation="MFSIRFNRWTIAAVALTVLAMPASLSAQTPDHPADNSAQFPSRG
                     DLKSLTSSGSYLAARHASVERDASSAAAFYRSALRTDPKNNELMDRAFISSLADGDID
                     EAVKLADRILAIDKSNRVARLVVGVRDLKLKKYAAAQLNINQSIRGPITDLVATLLSG
                     WASYGAGDAKTAIANIDKLTGPEWYPLFKDLHAGMILELAGKEKDAGMRLERAHKLDD
                     SMLRVADDYARWLSRNKDAAAATAVYDAFDKKLPRHPLVLEGLRETKAGKKLSPLVDS
                     PQTGAAEALYGIGATLTRRGGEDLALVYLQLALYLHPNHSLALLSLADLYESVKKPAM
                     AIKVYERMPASSPLKRNAQIQLATNLDAAERSDEAIKILKTVTTEDPKDIEAIMALGN
                     IERGRKKFADCVTTYSQGIDALPEANEKTNWIYYYYRGICEERSKQWSKAEADMRKAL
                     DLQPEQPHVLNYLGYSWIDQGINLDEGMKMIKRAVDQRPDDGYIVDSLGWAYFRIGNF
                     EDAVKNLERAIDLKPEDPTINDHLGDAYWRIGRTLEAKFQWAHARDLKPEPEELPKIE
                     AKINNGLPDDASSSAASADKKKEDGKGG"
     gene            2178..3062
                     /locus_tag="VGA15_04140"
     CDS             2178..3062
                     /locus_tag="VGA15_04140"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX9586916.1"
                     /translation="MPALTEDARAKVNLTLRVVGRRVDGYHDLESMVAFADCADRLSL
                     TPGSQLNLRTTGPLAQDCGETADNLVFKAAELLGERVPDLKLGDFTLDKVLPVAAGIG
                     GGSADAAAALRLLAQTNGLAIDDARLIEVAKLTGADVPVCLASRPCVMTGVGETLLPL
                     SLPKLPCVMVNPRVPVATRDVFKALGLRNGELLVGVADVLRATAWPEDGASVEDWVEV
                     LATGSNDLEAPATRIQPVIGEVLSALSATNGAWLVRMSGSGATCFAIYENTPEAQRAS
                     QKIQRDHPQWWVHAGTLS"
     gene            3184..3531
                     /locus_tag="VGA15_04145"
     CDS             3184..3531
                     /locus_tag="VGA15_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012755896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9586917.1"
                     /translation="MPVADTDLLKRLYQRFNARDMEAVLATMHGDVLWANGMEGGHVS
                     GHEGVRGYWTRQWAMIDPHVEPVSFSTPADGSTEVEVHQIVRDLNGSILSDKMVRHIF
                     RMDDGLIRRFDIG"
     gene            complement(3606..4547)
                     /gene="argC"
                     /locus_tag="VGA15_04150"
     CDS             complement(3606..4547)
                     /gene="argC"
                     /locus_tag="VGA15_04150"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX9586918.1"
                     /translation="MVAKIFIDGEAGTTGLQLRDRLAGRRDFEILTIAPDKRKDPDER
                     KRFLNAADIAILCLPDEAAKESVSLIENNTTKVIDASTAHRVADGWTYGFAEMDRDQS
                     KKIAGAMRLSNPGCWPQGGIATLRPLVSAGLIPVDFPVIAHGISGYSGGGRSMIADYE
                     QRGESANEFSLYGLSLTHKHLPELQTYSKLSKTPLFTPAVGNFRQGMIVTVSMQLWTL
                     SQVPTGSQLHAAIADHYAAISDGFVEVAPLSDTSGLDPELYNDTNRMRLHVFANDQKA
                     QAVLVAVYDNLGKGASGAAVQNLNLMLGVAADTSLAA"
     gene            complement(4642..5292)
                     /locus_tag="VGA15_04155"
     CDS             complement(4642..5292)
                     /locus_tag="VGA15_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX9586919.1"
                     /translation="MGFAALNPSYDISLFLLAALIIAAIPGPGIFYVAARTLSGGRRA
                     GIASTFGTALGGLAHVIAGGLGVSAIILASAQLFAVLKLAGALYLIWLGIRTVREAGH
                     LPTEPTGAIGFKRAFQDGIVVEALNPKTAAFFLAFIPQFLEPAAGHPALQFMTLGLIS
                     VTLNTFADVIVIVAASAARAGLVRRPTLVRRLRQGSGLFIAGLGISLALARRPATS"
     gene            complement(5360..>5506)
                     /locus_tag="VGA15_04160"
     CDS             complement(5360..>5506)
                     /locus_tag="VGA15_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9586920.1"
                     /translation="TKHRALEDTIHRAQHYGAMAVDALALFPASPMKTALEQVVAFCL
                     ARSH"
BASE COUNT         1102 a         1663 c         1732 g         1009 t
ORIGIN      
        1 cgctttcaga tcaacgccca gaattgcgtc cattgcaaaa cctgcgacgt gaaggacccg
       61 aacggcaata tcacctgggt tccgccggag ggtgggggcg gaccgaatta tgaggcgatg
      121 taaccacgat tgcgtgagcc gctgcgcgcc aatgcggccc ccggccacga taccgccaca
      181 ctgccagcgt tttcaggcga cctcggcggc catcgagcct tgctgccaat cgattcgcca
      241 cccgtatcct tgcggttgag cgccaaacgc ggcattgtca cacggcttga tgccgcccgt
      301 tgggctcgca tcgggatcga ttgtagatgc cgccgtaaat tcctgaaagc taaggcgaac
      361 cctgatgttt tccattcgtt ttaatcgctg gacgatcgcc gcagtcgctc tcaccgtgct
      421 ggctatgccc gcttcactgt cggcgcagac gcctgatcat ccggccgaca actcggcgca
      481 atttccctcc cggggcgatc tgaaatcgct gacgtcgtcg ggcagctatc tggccgcccg
      541 tcatgccagc gtggagcggg acgccagctc ggcggcggcg ttttatcgct cggcgcttcg
      601 taccgatccg aaaaacaacg aattaatgga tcgcgcgttc atttcatcgc tcgcggatgg
      661 cgatattgac gaggccgtca agctcgccga tcgaatcctg gcgatcgaca aatccaatcg
      721 cgtggcccgg ctggtggtcg gcgtgcgcga tctgaaactg aagaaatacg cggccgcgca
      781 gttgaacatc aatcagtcga tacgcggacc gatcacggat ctggtggcga cgctgctgtc
      841 gggctgggcc agttacggcg ccggcgatgc caagacagcc attgccaaca tcgacaagct
      901 gacgggcccg gaatggtacc cgctgttcaa ggacctgcac gccggcatga tcctcgaact
      961 tgcgggcaag gaaaaggacg ccggcatgcg ccttgagcgg gcacacaagc tcgacgattc
     1021 gatgctgcgt gtggccgacg actatgcgcg ctggctgtcg cgcaacaagg atgcggcggc
     1081 ggcgaccgcc gtctacgacg cattcgacaa gaagctgccg cggcatccgc tggtattgga
     1141 aggcctgcgc gagaccaagg ccggcaagaa gctgtcaccg ctggtcgatt cgccgcagac
     1201 cggcgccgcc gaggcgctgt acggcatcgg cgcgacgctg acgcgccgcg gcggcgagga
     1261 tctcgcgctg gtctatttgc agctcgcact gtacctgcac cccaatcatt cgctggcgct
     1321 gttgtcgctg gccgatctct atgagtcggt gaagaagccg gcgatggcga tcaaggtcta
     1381 cgagcgcatg ccggcgagtt cgccgctgaa gcgcaacgcg cagattcaac tcgcgaccaa
     1441 cctcgacgcc gccgagcgca gcgatgaagc catcaagatc ctgaagaccg tcacgacgga
     1501 agatcccaag gatatcgaag ccatcatggc gctcggcaat atcgagcggg gccgcaagaa
     1561 gttcgccgac tgcgtcacga cctactcgca gggcatcgat gcactgccgg aggccaatga
     1621 aaaaaccaac tggatctatt actattatcg cggcatctgc gaggagcgct ccaagcagtg
     1681 gagcaaggcc gaggccgata tgcgcaaggc gctcgatctg cagcccgagc aaccgcatgt
     1741 gctgaactat ctcggttatt cgtggatcga tcagggcatc aatctcgacg agggcatgaa
     1801 gatgatcaag cgcgccgtcg atcagcggcc cgacgacggc tacatcgtgg actcgctggg
     1861 ctgggcctat ttccgcatcg gcaatttcga agacgcggtg aaaaatctcg agcgcgcgat
     1921 cgatctcaag cccgaagacc cgacgatcaa cgaccatctc ggcgacgcct attggcgcat
     1981 cggccgcacg ctggaggcga aattccagtg ggcccatgcc cgcgacctga aaccggagcc
     2041 ggaggaattg ccgaagatcg aggccaagat caacaacggg ctgcccgatg atgcttcttc
     2101 ctccgcggcc tccgcggaca agaagaaaga agacggcaag ggcggttgag ggcggctgtt
     2161 cgaagggctg ctcgccgatg ccggcgctaa ccgaagatgc gcgtgccaag gtcaacctga
     2221 cccttcgggt ggttggacgc cgggtcgatg gctatcacga tctcgaaagc atggttgcgt
     2281 tcgccgattg cgccgaccgg ctcagcctga cgcccggatc ccaactcaat ctcagaacca
     2341 ccgggccgct ggcgcaggac tgcggcgaga ccgccgacaa cctggtgttc aaggcagcgg
     2401 aattgcttgg cgaacgcgtg cccgatctca agcttggcga tttcaccctc gataaggtgt
     2461 tgccggtcgc ggccggcatc ggcggcggct cggccgatgc cgccgcggcg ctgcggttgc
     2521 tggcacagac gaacggtctt gccatcgacg acgcgcgcct gatcgaggtc gcgaaattga
     2581 ccggcgccga cgtgccggtc tgtctggcct cgcgtccctg cgtcatgacc ggcgtcggtg
     2641 aaaccctgct gccgctgagc ttgccgaagc tgccttgcgt gatggtcaat ccgcgggttc
     2701 cggtcgcgac ccgcgacgtg ttcaaagcgc tgggtttgcg caatggcgag ttgctggtgg
     2761 gcgtcgccga cgtgctccgg gcaacggcct ggccggaaga tggcgcgtcg gtcgaagact
     2821 gggtcgaagt actcgccacc ggctccaacg acctcgaggc tccggcaacg cgcattcagc
     2881 cggtcatcgg cgaggtgctc tcggcgctca gcgccaccaa cggggcatgg ctggtgcgaa
     2941 tgtcgggatc gggcgctacc tgtttcgcga tctatgagaa cacccccgag gcgcagcgcg
     3001 cgtcgcagaa aattcagcgc gatcacccgc agtggtgggt gcatgcgggg acgctgagtt
     3061 agaagtgagc gggatgggtc cagattgaat cgcgactgta ttcacttgac ctacgccgat
     3121 tgccttgcaa tctcgtgatg gccgggcctg gtcccggcca tttgacgtta tcgtgccgtc
     3181 atgatgccag ttgccgacac agaccttctg aaacggcttt accaacggtt caacgcccgt
     3241 gacatggagg cggttctggc caccatgcat ggtgacgtgc tatgggccaa tggaatggaa
     3301 ggcgggcacg tctcagggca tgaaggtgtc cgaggttact ggacgcgaca atgggcgatg
     3361 atcgatcctc atgttgaacc agtgagcttc tcaacgccgg ctgacggcag caccgaggtc
     3421 gaagttcacc agatcgtgcg cgacctcaac ggcagcattt tatccgacaa gatggtccgg
     3481 catatttttc gtatggatga cggcctgatc aggcgttttg atattggcta gactacggcg
     3541 tgaactcgtc agcggctaat aacgataggt cactgctcgt ccacatttcg gacagcgccg
     3601 ccaagtcagg ctgcgagtga ggtgtccgcg gcaacaccca acatcaaatt gaggttctgc
     3661 acagcggcgc ccgaggctcc cttgccgaga ttgtcgtaga cggcgaccag aacggcctgc
     3721 gctttctggt cattggcgaa gacatgcaga cgcatgcggt tggtatcgtt gtaaagttcg
     3781 gggtccagtc cggacgtgtc cgaaagcggc gcgacttcga cgaagccgtc cgaaatcgcc
     3841 gcgtaatgat cggcgatggc agcatgaagt tgactgccgg tcggaacctg cgagagagtc
     3901 cacaattgca tcgacacggt gacgatcatg ccttgccgga aattgccgac tgccggagtg
     3961 aacaggggcg tcttgctgag cttggaatag gtctgtagct cgggcaaatg cttgtgcgtg
     4021 agcgacaggc catagagtga gaactcgttg gcactctcac ccctctgctc atagtctgcg
     4081 atcatcgatc ggccgccacc tgaatagccg gaaatgccgt gggcgatcac cggaaaatcg
     4141 acagggatta acccggcgct gaccaacggc cgcagcgtcg cgatgccgcc ctgcggccaa
     4201 cacccgggat tgctcaaccg catggcgccg gcaatcttct tcgactgatc cctgtccatc
     4261 tcggcaaagc cataagtcca gccatcggcg acgcgatgtg cggtcgaagc gtcaatcact
     4321 ttggtcgtat tgttctcgat caggctcacg ctttcctttg ccgcttcgtc gggcagacag
     4381 aggatcgcta tgtcagcggc gttgaggaag cgtttgcgct catccggatc cttgcgttta
     4441 tcaggggcga tcgtaaggat ttcaaagtcg cgccgcccgg ccagccggtc acggagttgc
     4501 aaccccgtcg tcccagcttc gccgtcgata aatatctttg cgaccatctc tcgcttccat
     4561 ccgatcaccg agtgcgcggg catactaggt gccgctgcgg cttgttgcaa tcttctggcg
     4621 gcgcgccagg gagtctgcac cctagcttgt cgcgggccgg cgcgcgagcg cgagcgaaat
     4681 tccgagccct gcaatgaaaa gcccggagcc ctgccgcagg cgccgaacca aagttggcct
     4741 gcgcacgaga cccgcgcggg ccgcagaggc tgcgacgatc acaatgacgt cggcgaacgt
     4801 gttgagtgtc accgagatca gacccagcgt catgaactgc agcgcggggt ggccggcggc
     4861 cggttccaga aattgcggaa tgaacgccag gaagaaagcc gcggtcttgg gattgagcgc
     4921 ttcgaccacg atgccatcct ggaatgcccg cttgaatccg atggcgcccg tcggctccgt
     4981 cggcagatga ccggcttcgc ggacggttct gatgccaagc cagatcagat agagggcacc
     5041 ggccagcttg agcacggcaa agagctgcgc actggcaagg atgatcgccg agacgccaag
     5101 accgccggcg atcacgtgcg caaggccacc caatgcggtg ccgaaggtgg atgcgatacc
     5161 ggctcgtctg ccgcccgaca gcgttctcgc tgccacgtag aaaatgccgg gaccgggaat
     5221 ggcggcaatg atgagcgcag ccagcagaaa cagcgaaata tcgtaggatg ggttgagcgc
     5281 agcgaaaccc atcaacggct tccgatgaag tgatgggttt cgctgcgctc aacccatcct
     5341 acgactcttg cgtttctgtt caatgcgatc tcgccaggca gaatgccacc acctgctcga
     5401 gcgcggtctt catgggagag gccggaaaca gcgccagcgc atcgaccgcc atggcgccgt
     5461 aatgctgggc gcggtggatg gtgtcctcga gcgcgcggtg cttggt
//
LOCUS       DASWGM010000136         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2369590,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000136 DASWGM010000000
VERSION     DASWGM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2369590"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(468..1325)
                     /locus_tag="VGA15_04165"
     CDS             complement(468..1325)
                     /locus_tag="VGA15_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586921.1"
                     /translation="MGGLAVGFHPGRRIRRSGDRCFYRRRNRRIGLKLRRLYRARGRM
                     VMLIVGVLVVSMFMLMIVMIMMFRAAVVMSAVVGVGVQIFGVQIFGVQVFGVQLFRVQ
                     AFGAFRAFMRPGGLRRIGACVLDDLALDALAIAAAARVAVARPAAVGAVFALLLGLAM
                     GAFVCFDQRLAIGDRDLIIVRMDFAEGEKAVAVATVLDEGRLQRRLYPRDLGEIDVAA
                     QLFALGGLEIKLFDAIAADHDNPGLFRVGSIDQHFVGHFGTHGGGGRVQPGARIARPG
                     DATVHLIRG"
     gene            1299..>2948
                     /locus_tag="VGA15_04170"
     CDS             1299..>2948
                     /locus_tag="VGA15_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX9586922.1"
                     /translation="MEAHGESAHDERTREEHAEENAAHADDAASAEHAGEDHGASPHD
                     GHSAQPHGDENHDDDDDGDDAEEEVVESVGGDDVMEEVPERAFRPRRQYKIQEVIKRR
                     QVMLVQVVKEERGNKGAALTTYLSLAGRYAVLMPNTARGGGISRKITSAQDRSRLKEV
                     VQDLDVPEGMGIILRTAGASRTKPEIKRDFEYLIRMWETVRDMTLKSQAPTLVYEEGS
                     LIKRSLRDLYNKEIDEIQVAGEAGYQEARDFMKMLMPSNVRAVRQYRDGQPLFSRMGV
                     ESQLDAMFSPTVQLRSGGYIVINQTEALVSIDVNSGRSTREHHIEDTALKTNLEASEE
                     VARQLRLRDLAGLIVIDFIDMDEKRNNRSVERKLSDCLRQDRARIQVGRISHFGLLEM
                     SRQRIRASVLESSTEPCSQCGGSGHVRSVSSVALQLLRGLEEILMKGATHNLVVRTRT
                     DVALYVLNHKRGHLRDLENSFKVTLAVIADPTVSGQQSFIIDRGEQVHTLEAAKALLA
                     AQVAAFPAYIEEASDDEEPFDIETESEVETEETEGLADDAPV"
BASE COUNT          599 a          962 c          929 g          458 t
ORIGIN      
        1 aaggggctga cggctaaaac tcccggttgt gtcgcgttca gcctcccggt gcagcgccgg
       61 agccttcgaa acttcggaga ttccagcgtc tttccgatcc ctccgcagcc agcgcagcgc
      121 gcggctgaca aggagcggcg gtttcgagcc cgagcggcgt ccggtcccag gtcaccagat
      181 tgctccaggg acggcttcag acacggtata accgattatc cgggccttaa gggcccgata
      241 tgcgatggcc ggcgagcgcc tcggcgccgc gtcgggcttt cagcgacaga caaggcggcg
      301 cctttgccgc ccacgtgttc agacgggccg acgcttgatg cgccagacag tgttgccgac
      361 catgatccgg gtaagaactg cgccgaggcg gagcgagagc tccgcgcggt gctttgctcc
      421 gggatcccgt tcggcccggc gccccgggtt gcgttttggc cttcccctca cccccgaatc
      481 aggtggaccg tcgcgtcacc cggccgcgcg atacgcgctc ccggttgaac gcgcccgccg
      541 ccgccatgag ttccaaaatg cccaacaaaa tgttgatcga tgctacccac ccggaagaga
      601 cccgggttgt cgtggtccgc ggcaatcgcg tcgaagagtt tgatttcgag accgcccagc
      661 gcaaacaact gcgcggcaac atctatctcg ccaaggtcac gcgggtagag ccgtcgctgc
      721 aggcggcctt catcgagtac ggtggcaacc gccacggctt tctcgccttc agcgaaatcc
      781 atccggacta ttatcagatc ccggtcgccg atcgccaggc gctgatcgaa gcagacgaac
      841 gcgcccatcg cgaggccgag gaggagagcg aaaaccgctc caaccgccgc cggccgcgcc
      901 accgcaactc gcgccgccgc ggcaatggcg agcgcatcca gagcgagatc atcgagaacg
      961 caagcaccga tccgtctcag gccgcccggc cgcatgaagg ccctgaacgc accgaaggct
     1021 tgcacccgga agagttgcac accgaacact tgcacgccga agatttgcac gccgaagatt
     1081 tgcacgccta ccccgacgac ggcggacatc acgacggcgg cgcggaacat catgatcatg
     1141 acgatcatga gcatgaacat gctcacgacg agcacgccca cgatgagcat gaccatgcgc
     1201 ccgcgcgcgc ggtacaagcg gcggagtttc agcccgatac gccggtttcg gcggcggtag
     1261 aagcatctgt cgccgctgcg ccggatgcgc cggccagggt ggaagcccac ggcgagtccg
     1321 cccatgatga gcgcacccgc gaggagcatg cggaagaaaa cgcggcccac gccgacgacg
     1381 ctgcatcagc cgaacatgcc ggtgaagacc acggtgcttc gcctcacgac gggcattcgg
     1441 cccagccgca tggcgacgag aaccacgacg atgacgacga tggcgacgac gccgaggaag
     1501 aagtcgtcga atccgtcggc ggcgacgacg tcatggaaga ggtgccggag cgtgccttcc
     1561 gtccgcgccg gcaatacaag atccaggaag tgatcaagcg ccgtcaggtg atgctggtgc
     1621 aggtggtcaa ggaagagcgc ggcaacaagg gcgcggcgct gacgacctac ctgtcgctgg
     1681 ccggccgcta tgcggtcttg atgcccaaca ccgcgcgcgg cggcggcatc agccgcaaga
     1741 tcactagcgc gcaggatcgc tcgcgcctga aggaagtggt gcaggatctc gatgtgcccg
     1801 agggcatggg catcatcctg cgcaccgcgg gcgcgtcgcg caccaagcct gaaatcaagc
     1861 gcgacttcga atatctgatc cgcatgtggg aaaccgtgcg cgacatgacc ttgaagtcgc
     1921 aggcgccgac gctggtctac gaggaaggct cgctgatcaa gcgttcgctg cgcgatctct
     1981 acaacaagga aatcgatgaa atccaggtcg ccggcgaagc cggctatcag gaagcccgcg
     2041 atttcatgaa gatgctgatg ccgtcgaacg tgcgcgcggt caggcaatat cgcgacggcc
     2101 agccgctgtt ctcgcggatg ggcgtcgaga gccagctcga tgcgatgttc tcgccgaccg
     2161 tgcaactgcg atccggcggc tacatcgtaa tcaaccagac cgaggcgctg gtttcgatcg
     2221 acgtcaactc cggccgctcc acccgcgaac atcacatcga ggataccgcg ctcaagacca
     2281 accttgaggc ttccgaagaa gtcgctcgcc agctgcgcct gcgcgatctc gccggcctga
     2341 tcgtcatcga cttcatcgac atggacgaga agcgcaacaa ccgctcggtc gagcgcaagc
     2401 tcagcgattg cctaaggcag gaccgcgccc gaatccaggt cgggcgcatc tcgcatttcg
     2461 gcctgctcga aatgtcacgt cagcgcattc gcgccagcgt gctggagagt tcgaccgagc
     2521 cctgctcgca atgcggcggc agcggtcatg tgcgttcggt atcgtcggtg gcattgcagc
     2581 tgctgcgcgg tcttgaggaa atcttgatga agggcgcgac ccacaatctc gtggtgcgca
     2641 cccgaaccga cgtcgcgctt tacgttctca accacaagcg cggccatctg cgcgatctcg
     2701 aaaacagctt caaggtgacg ctggctgtca tcgccgaccc gaccgtcagc ggccagcagt
     2761 cgttcatcat cgatcgcggc gagcaggtgc atacgctgga ggccgccaag gcgctgctgg
     2821 cggcgcaggt tgcggctttc ccggcctata tcgaagaagc ctccgacgac gaagagccgt
     2881 tcgacatcga aaccgagtcg gaagtggaaa ccgaagagac cgaagggctg gccgacgatg
     2941 cgccggtc
//
LOCUS       DASWGM010000137         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1674130,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000137 DASWGM010000000
VERSION     DASWGM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674130"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..187
                     /locus_tag="VGA15_04175"
     CDS             2..187
                     /locus_tag="VGA15_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586923.1"
                     /translation="MPAFSFEKISPPIRRGPVPAIARKQRGVIVQILDRFVEARVKRT
                     LRAEKRVIARREKKPSK"
     gene            complement(229..1122)
                     /locus_tag="VGA15_04180"
     CDS             complement(229..1122)
                     /locus_tag="VGA15_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX9586924.1"
                     /translation="MTSLTPSTSRATFAIGSEQTARRVVDLLTESFFEGQAAIAAFER
                     SDGRWGITVHFAEAPDQAQVRDLVGIAAGDDVAADITFDTVEAKDWVKATLEELVPVR
                     AGRFIVHGHHDRSKVPPNKLGIEIEAALAFGTGHHGTTRGCLLLLDSVLKARRPQRVL
                     DLGTGTGVLAIAAAKALRVKVLASDIDPLAARVARDNARLNGAGDLVETIAAVGFSAP
                     DFAGHGRFDLVLANILANPLRQMATPMAKHLAASALVILSGLLPHQAQGVIAAYRARG
                     LVLERHLMIEGWSSLLMRYAE"
     gene            complement(1937..2197)
                     /locus_tag="VGA15_04185"
     CDS             complement(1937..2197)
                     /locus_tag="VGA15_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586925.1"
                     /translation="MHDSHSDQGVPSKYFFHILLNGSHSGQSDGLLFRDKAAAWREAS
                     MSCGEIIREMDGKIGPGCNWRMEVADHTGQAIYRFTFTAEEL"
     gene            complement(2428..>3799)
                     /locus_tag="VGA15_04190"
     CDS             complement(2428..>3799)
                     /locus_tag="VGA15_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9586926.1"
                     /translation="GGGGAHFGGMRHFGGGGGVRHFSGGGGRHFSGRFTAPRSTAGAI
                     SRGGGRTTFSSNGSNRASGLNRNANLRSGAVHRALNSRAVAGALHNRAALRNPGTRAG
                     IAAIAATAGWHSGGGWWRHGHGGFGWVGPLFWPFAYYDIYDYALWGYGYDPSFWDYGY
                     GDIYAGLFAPYGYDDLTGYFPGVSTQQASHAAANPAPDQLTQMCGDDGRDIAGLPIDQ
                     IQQAIAPNEVQRAALDDLANASVKAAQIIKAACPTQIALTAPARLAAMQQRIEAMIAA
                     VGTVQPPLDKFYGLLNDEQKSRLNALGQRSKSAAQNAGGSLAQSCGATQAGVTDWPTA
                     EIDARLHPTDAQRASLTALQNANAKAEEMLKNSCQADDALTPPARLAAVGKRLDVMLQ
                     AVKTVRTALDDFYGKLTDEQKAQFEAIGQGRAALTSQAAAPTHHRRRHHAGVGGIIRH
                     FMAFGW"
BASE COUNT          713 a         1194 c         1172 g          720 t
ORIGIN      
        1 aatgcctgct ttttcgtttg agaagatttc gccgccgatc cgccgcggcc ctgttcctgc
       61 cattgccagg aaacaacggg gcgtcatcgt ccagatcctg gatcgtttcg tcgaagcgcg
      121 ggtgaagcgg acgctgcgcg ccgagaagcg cgtcatcgcc cgccgcgaaa aaaaaccgtc
      181 gaagtaggcc gttcttgacg gtacgacggt catgggcgta gccggtcctc attcagcgta
      241 acgcatcaag aggctgctcc agccttcgat cataagatgc cgttccagaa caagcccgcg
      301 cgcgcggtag gcggcgatca cgccttgagc ctgatgcggc agcagccccg acagaatgac
      361 cagcgccgat gctgccagat gcttcgccat cggcgttgcc atttgccgca agggattggc
      421 cagaatattc gccagcacca gatcgaatcg gccgtggcct gcaaaatccg gcgctgaaaa
      481 tcccactgcc gcgatcgtct ccaccagatc accggcaccg ttgagccggg cgttgtcgcg
      541 cgccacccga gccgcgagcg gatcgatgtc gctcgccagc actttcaccc gcaaggcttt
      601 cgccgcagcg atggccagca cgccagtgcc tgttccaagg tcgagcacgc gctggggacg
      661 gcgagccttc agcacggaat cgagcagcaa cagacagccg cgcgtggtgc cgtggtggcc
      721 ggtgccgaac gccagtgcgg cctcgatctc gatgccgagc ttgttgggcg gcacttttga
      781 gcggtcatga tgaccgtgca cgatgaaacg cccggcgcgg acgggcacga gttcttcaag
      841 cgtagccttg acccagtcct tggcttccac cgtgtcaaag gtgatgtccg ctgcgacgtc
      901 gtcgccggcg gcgatgccga cgaggtcgcg gacctgtgcc tgatcgggtg cttcggcaaa
      961 atgcacggtg atgccccagc gcccgtcgga tcgttcgaac gcggcgatgg cggcctggcc
     1021 ctcaaaaaag ctctcggtca ggagatcgac gacgcgccgc gcggtctgtt cgctgccgat
     1081 cgcgaaggtc gcgcggctgg tgctgggggt gagggaggtc atcaaaggtc cgggaaatcg
     1141 atgctgtctt aaaatgcgtt gtccttcaca tccattaccc ggtaatcggg tggtgcaaca
     1201 tagctgcccg cctgtgacgg ctcaacactc accgccggga gcgttcccac gcaaaaaatc
     1261 cagtccgcaa aacgagatgc ggccgcgggt tcacagcctg tgaactcgaa acgcaagagt
     1321 ttttcacagc gttatatacg gaaacatacc tggtttttca cacgcctgcc cccaacgttc
     1381 tccacaatgc ggtgctgctc gcctctggag cttcgggaca ttccgctcca cgccttggaa
     1441 agcagcggcc acgtctggga gtgtcgcctt gtgagagagc gcgaaccggc tgacccaagt
     1501 gttcacagcc tgtgaactcg aaacgcaggg gttgctcaca gtgttgtgca ctgaagcata
     1561 cgcggctttt tcacaggccc gtccccagct tttgcacgaa cctggcggtc cttgcgggtg
     1621 gtccgaagga ccagatcctg gatgaccgct cctccatgga gttatgaatt acgtctccac
     1681 cgacttatca gcgacttctc caccgagtta tcgccagctc atcaaccggt tctccacaac
     1741 ttgtccaccg gcttgtccac cggttgtacg ggtcattgcg agaatcggca acagcgaagc
     1801 aacccgggct catcagcgcg aggcggctcg acagagaacg gcagcggagc gttgattccg
     1861 gcgtcgggag ctcgaaaacg ccggttactt acgatgagtg caaggcgaag atcaaaaccc
     1921 tcgcgcgaca gttcgactac aattcttcag ccgtgaacgt gaatctgtaa atcgcctgac
     1981 cggtatgatc ggccacttcc atccgccagt tgcagcccgg gccgatttta ccgtccatct
     2041 cccggattat ttcgccgcat gacatggaag cctcccgcca tgcagcggcc ttatcacgaa
     2101 ataacagacc gtccgattgg cccgaatggc ttccgttcag gaggatgtgg aaaaagtatt
     2161 tggaggggac gccttggtcc gaatgagaat cgtgcatgcg tttctgccgt acgatcaggc
     2221 gggagcacta tcgaactccc agtcaccgat gattgccggg ggccgtacgg tgatggggaa
     2281 taataccacc gtctgttgga aagacgacat ataagaaagt gcacgagaag cagaacgatt
     2341 ctaaatttgc tgacgcttac ccgggaacct aggattgaac atcggttccc ggatcggcgc
     2401 ttcgccctgg aatcatcaac ggcgcattca ccacccgaag gccataaaat gccggatgat
     2461 gccgccgacg ccggcatgat ggcgccggcg atggtgcgtg ggcgccgcag cctggctggt
     2521 cagggcggcg cggccctggc cgatcgcttc gaactgcgcc ttctgttcgt cggttagttt
     2581 tccatagaaa tcatccagcg ccgtgcgcac cgtcttgacc gcctgcagca tgacatccag
     2641 ccgttttcca actgcggcga ggcgggcagg cggcgtcagc gcatcatctg cctggcagga
     2701 attcttgagc atctcctccg ccttggcgtt ggcattctga agggcagtga gacttgcgcg
     2761 ctgcgcgtcg gtcgggtgca gcctggcgtc gatctcggcg gtcggccagt ccgttacacc
     2821 ggcttgcgtg gcgccgcagc tctgggcgag cgagcctccg gcgttctgcg ccgccgactt
     2881 gctacgctgt ccaagcgcgt tgagccggga cttctgctcg tcgttcagta gcccgtaaaa
     2941 cttgtccaat ggcggctgta ccgtaccgac cgcggcaatc atcgcttcga tgcgttgctg
     3001 catggctgcg agccgggccg gcgccgtcaa cgctatctgt gtggggcacg cggccttgat
     3061 gatttgggcc gccttcaccg aagcgttggc gaggtcgtcg agcgccgcgc gctggacctc
     3121 gttgggcgca atagcctgct ggatctgatc gatcggcaag ccggcgatgt cacggccgtc
     3181 gtcgccgcac atttgcgtca gttggtcagg cgccggattg gcggctgcgt gcgacgcttg
     3241 ttgcgtgctg acccccggga agtaaccggt gaggtcgtca tagccgtagg gagcgaacag
     3301 cccggcatag atgtcgccgt agccgtagtc ccagaatgag gggtcgtagc catagcccca
     3361 caaggcatag tcgtagatgt cgtaataggc gaacggccag aacagcgggc cgacccagcc
     3421 gaagccgccg tggccgtgcc gccaccagcc gccgccgcta tgccacccgg cggtcgccgc
     3481 tatcgcagcg atcccggcgc gcgtgcctgg attgcgtaaa gcagccctgt tatgcaaggc
     3541 gcccgccacc gcgcgcgaat tcagcgcgcg gtgcacggcg ccggatcgca gattggcgtt
     3601 gcgattgagg ccggatgccc tgttgctgcc gttgctgctg aacgtcgttc tgccgccgcc
     3661 acgagaaatc gcgcctgcgg tcgaccgcgg ggccgtgaac ctgccactga agtgccgccc
     3721 gccgccgccg ctgaagtgac ggacgccacc tccaccgccg aagtgccgca taccgccgaa
     3781 atgcgcgccg ccgccaccg
//
LOCUS       DASWGM010000138         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1674131,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000138 DASWGM010000000
VERSION     DASWGM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674131"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VGA15_04195"
     CDS             <1..376
                     /locus_tag="VGA15_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX9586927.1"
                     /translation="NRVDEIILFHRLQKSEMGRIVEIQFGRLQKLLEERKIVLTLDAA
                     ARDWLAAKGWDPAYGARPLKRVIQRHVQDPLAEMILAGEVRDGDHVAISVEGNVLTFN
                     GKAPQTAEIAQFEAPIPKRKLN"
     gene            complement(415..675)
                     /locus_tag="VGA15_04200"
     CDS             complement(415..675)
                     /locus_tag="VGA15_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586928.1"
                     /translation="MKRVTRDDITKVVGPADDVTIAEIIGTGATVDELAEAQAWIVND
                     EPLLNAGKPLASGRVRELIDILAEFEPSDDDDKEAGPAVGSI"
     gene            complement(745..1104)
                     /locus_tag="VGA15_04205"
     CDS             complement(745..1104)
                     /locus_tag="VGA15_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEX9586929.1"
                     /translation="MDNWSNAKLCDVANLILGACLFFSPWMFGFGAGKASENANIAGI
                     AIAVLAIAALAAFAIWEEWLNLIVGLWTLVSPWVLGFQGTTAMTVHVIIGAAVAILAA
                     VEIWIMSQHPPRLTASH"
     gene            complement(1413..1778)
                     /locus_tag="VGA15_04210"
                     /pseudo
     CDS             complement(1413..1778)
                     /locus_tag="VGA15_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1832..>2080)
                     /locus_tag="VGA15_04215"
     CDS             complement(1832..>2080)
                     /locus_tag="VGA15_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586930.1"
                     /translation="VGSTGQSTGPHVHYEILVNGRFVDPMRIKLPRGRSLDGPLMAGF
                     EKERDRLDTMMNNRGGVARVSDAMGSIGTPQGRQVSNR"
BASE COUNT          402 a          670 c          618 g          390 t
ORIGIN      
        1 caaccgcgtc gacgagatca tcctgttcca ccgcctgcaa aagagcgaga tgggccggat
       61 cgtcgagatc cagttcggcc ggctgcagaa gctcctggag gagcgcaaga tcgtgctgac
      121 gctcgatgcc gcggcccgcg actggctcgc ggccaagggc tgggatcccg cctacggcgc
      181 ccgcccgttg aagcgggtga tccagcgcca tgtgcaggat ccgctggcgg agatgatcct
      241 agccggcgag gtcagggatg gcgatcacgt cgcaatttcc gtcgaaggca acgtgctgac
      301 cttcaacggc aaggcgccgc agaccgcgga aatcgcgcag ttcgaagcgc cgataccgaa
      361 acggaagctg aactgagttt ccttccctct cccgcagggg agagggaagt tcgctcagat
      421 cgaaccgacg gcagggccgg cttccttgtc atcgtcgtcg ctcggctcga actccgcgag
      481 gatatcgatg agttcgcgaa cgcggccgct cgccagcggt ttgccagcgt tcagcaacgg
      541 ttcgtcgttg acgatccagg cctgagcctc cgccagttcg tccaccgtcg cgccggttcc
      601 aatgatttcg gcgatggtca catcatcggc cgggccgacg actttggtga tgtcgtcacg
      661 cgtgacgcgt ttcatggctt tccatcctca agctgccgtg ggccgcccgc cggcgtcggc
      721 ggcgagcggg acgcaggcac ctagctagtg gctcgcggta agtcgcggcg gatgctggga
      781 catgatccag atttcgaccg ccgcgaggat tgctacagcg gcgccgatga tcacatgcac
      841 cgtcatcgcg gtggttccct ggaagcccaa tacccaaggc gaaaccagtg tccacaatcc
      901 cacgatgaga ttcagccact cctcccagat tgcgaaggcc gcaagggccg cgatggcgag
      961 caccgcgatc gcgatgccag cgatattggc gttttcagag gcttttccag caccgaatcc
     1021 gaacatccag ggtgaaaaga acagacacgc gccgaggatc aggttcgcga cgtcgcacaa
     1081 tttcgcattg ctccaattgt ccatggcacc ctccattgaa ggctattcga ttcaaccaaa
     1141 gaaccgccac cggagttccg ctcggctttg acgatattta atgaaacccg cggcgatccc
     1201 gcagtttccg ttctgaaggg tcagctagcg cagggagtat gcgtcgtacc atctgacgcg
     1261 ataagacgcg cagaccacga accgctggct tccagccatg aagcgagcgc ggccagcccc
     1321 tcaaagatca aggcggcaca gccggcccac ggagccaaat ccgagtgccc gggtacgcag
     1381 ctggcgtctc tcgttcaacc cgtatctcgc ttctatttct tcgtgacctt gtagatcgcg
     1441 ttctcgatgt ccgacgagaa gtagatcacc cctgttgcgc cgaccccgac gccggtcgga
     1501 atgttggtcg gcagaccgcc cggcgcaccg accagcccga tcggcagatt gccggcgatt
     1561 tcactaactt ttccggtctg cggatcgact tcgacaagcc gcttggcgcc gacctcggcc
     1621 acgaccagtt tgccgtcagg cgccagcgcg atgccttcgg gcattttcag gtccttggcg
     1681 agcacggttt tttcgccgtt gcttccgacc ttcgagacct gcccggcaaa ggcttccgtg
     1741 acgtagacat cgcccttcgt accgccgacc agccccaaat gacttgccgt ccgcgcggtc
     1801 aaattcagat gacagaaatt ctatcgaagt ttcagcgatt gctgacttgc cgcccctgcg
     1861 gcgtgccaat cgagcccatg gcatcggata cgcgggctac gccgccgcga ttgttcatca
     1921 tggtatcgag acgatcgcgc tccttctcga agcccgccat cagcgggccg tcgagcgagc
     1981 ggccgcgcgg cagcttgatg cgcatcggat cgacgaagcg gccgttgacc agaatttcat
     2041 aatggacatg gggcccggtc gactgcccgg tcgaaccgac
//
LOCUS       DASWGM010000139         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1674304,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000139 DASWGM010000000
VERSION     DASWGM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674304"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGA15_04220"
     CDS             <1..273
                     /locus_tag="VGA15_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEX9586931.1"
                     /translation="WAEAVKKAGSIERMKAIEALETGISIDAPSGKVTIDPPTHHCVL
                     DVHIAEVKDKKLKVLEDFAQQKPSDTAAVCDLIKNPTDNQQYVIKI"
     gene            313..1179
                     /locus_tag="VGA15_04225"
     CDS             313..1179
                     /locus_tag="VGA15_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9586932.1"
                     /translation="MDLIALLAIQVLYAIASLALISVGLAIIFGMMRVINLAHGEFLM
                     LGGYAAIVATSHGISIWIAMLVVAPIVVGIIGVIVELTIIRFLYGRMVDTMLATWGLS
                     LFLVGLTTAIFGNTTVGISAPLGSFQIGAYRTSGYTLFVIAVAAVVMAGIFGVLRWTR
                     LGLIARGTMQNANMAAALGVNPPRVYAVTFGIGAALSGLAGAVLAPVSGVFPTIGVAY
                     VAKSFITVIGGGAAILSGTVSASALFGSINQIATFATTPVFGEVALLATAIVLIRLLP
                     QGITGRFFRRGL"
     gene            1176..2252
                     /locus_tag="VGA15_04230"
     CDS             1176..2252
                     /locus_tag="VGA15_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9586933.1"
                     /translation="MNARALNLIGLGLVGIVGIAFLILTPRFVELETVLELTVYMIMA
                     ILALSLALIWGYGGILCFGQSAFFGLGAYAYAIAMFNIGESTVPLLLAIALPAAFAAL
                     LGYFMFYGRISDVYLGVITLTVTLILFNSVNSTAGPEFHIGVARLGGFNGIPGIPPLN
                     FPGNKAAAIDLEGMFYLATAALLATYFGLRLLLASRFGRVIVGIRENERRAELLGYDP
                     RAYKLVTFTIGGALAGFAGCLFANWGNFVSPTIFGLAQSAQIIIWVIVGGRGTLIGPI
                     VGCIGIQWLNTALGANQPSGSDLLGKLLANAPLILGIILIAFVLLVPKGLVPTLGDWG
                     QSLLLRSRTRRLAAALKPKQAEEG"
BASE COUNT          376 a          727 c          698 g          500 t
ORIGIN      
        1 tgggcggagg cggtcaagaa agccggcagt atcgaacgca tgaaggccat cgaggcactg
       61 gaaaccggaa tcagcatcga tgcgccctcg ggcaaggtca cgatcgatcc tccgacccac
      121 cattgcgtcc tcgacgttca cattgccgaa gtgaaagaca agaaactcaa ggtgctcgaa
      181 gacttcgcgc agcagaagcc gtcggacacc gccgcggtgt gcgacctcat caagaatcca
      241 accgacaacc agcaatacgt catcaagatc taaaggccgg ttggccttca actctgtttg
      301 ggaatagcga acgtggatct cattgctctc ctcgcgatcc aggtccttta cgcgatcgcc
      361 agcctggcgc tgatcagcgt cgggctggcg attattttcg gcatgatgcg ggtcatcaat
      421 ctcgcacatg gagaatttct gatgctcggg ggctatgcgg cgatcgtcgc gaccagccat
      481 ggcatcagca tctggatcgc gatgctggtg gtggcgccca tcgtcgtcgg catcatcggg
      541 gttatcgtcg aattgacgat catccgcttc ctgtatgggc ggatggtcga caccatgctc
      601 gcgacctggg gcctcagcct gtttttggtg gggctgacga cggcgatctt cggcaacacc
      661 acggtcggga tctcggcacc gctcggcagt ttccagatcg gcgcctatcg caccagcgga
      721 tacacgctgt tcgtgatcgc ggtggcggcc gtcgtgatgg ccggcatttt cggcgtgctg
      781 cgctggaccc gccttggcct gatcgcccgc ggcacgatgc agaacgccaa catggcggca
      841 gcacttgggg tcaatccgcc gcgcgtctac gccgtcacct tcggcatcgg cgccgccctg
      901 tcgggtctcg ccggcgcagt gctggcgccg gtctccggcg tctttcccac catcggcgtc
      961 gcctatgtcg ccaaatcctt catcacggtg atcggaggcg gcgccgctat tctcagtggc
     1021 acggtttctg cttccgccct gttcggctcc atcaaccaga tcgccacctt tgccaccaca
     1081 cctgtgttcg gcgaggtggc gttgcttgcg accgccatcg ttctgatccg tctgctgccg
     1141 cagggcatca ccggtcgctt cttccggaga ggtctgtgaa cgcccgcgca ctcaatctca
     1201 tcggcctcgg actggtcggc atcgtcggca tcgccttcct gatcctgacc ccgcgtttcg
     1261 tcgagctcga gaccgtgctc gaacttaccg tctacatgat catggcgatc ctcgcgctca
     1321 gcctggcgct gatctggggc tacggcggca ttctctgttt cggccaatcg gccttcttcg
     1381 gattgggcgc gtatgcctac gcgatcgcca tgttcaacat cggcgagagc acggttccgc
     1441 ttttgctggc gatcgcgctg ccggcggcct tcgccgcgct gctcggctat ttcatgttct
     1501 atgggcgcat cagcgacgtg tatctcggcg tcatcacgtt gacggtgacg ctgatcctgt
     1561 tcaactcggt gaactccacc gccggcccgg aattccatat cggcgtggca cggctcggcg
     1621 gcttcaacgg cattccggga atcccgccgc tgaatttccc cggcaacaag gccgcggcga
     1681 tcgacctcga aggcatgttc tatcttgcga ccgccgcatt gctcgcaacc tatttcggac
     1741 tgcgcctgct gctggcgagc cgcttcggcc gcgtcattgt cggtatccgg gaaaacgaac
     1801 ggcgcgccga actcttgggc tacgacccgc gggcctacaa gctcgtgacc tttaccatcg
     1861 gtggcgcgtt ggccggtttt gccggatgcc tgttcgccaa ttggggaaat tttgtcagcc
     1921 cgaccatctt cggactggcg cagtccgcgc agatcatcat ttgggtcatc gtcgggggac
     1981 ggggtacgtt gatcggcccg atcgtaggct gcattggtat ccaatggctc aatacggcac
     2041 ttggagcgaa ccagccgagc ggttcggact tgttgggaaa gctattggcc aatgcgccgc
     2101 tcatcctcgg aataatcctg atcgccttcg tgctgctggt gccgaagggg ttggtgccga
     2161 cgctcggcga ttggggccaa tcgctgctgc tgcgctcgcg cacgcggcgg ttggctgccg
     2221 cgcttaagcc caagcaggcg gaggaggggt gatggccgcg ccgcttcttc aaacccgcga
     2281 tctcagcatg cgtttcggtg g
//
LOCUS       DASWGM010000140         5009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1118503,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000140 DASWGM010000000
VERSION     DASWGM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118503"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGA15_04235"
     CDS             complement(<1..248)
                     /locus_tag="VGA15_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9586934.1"
                     /translation="MITAAQLRAARVLLGIDQRRLAELSGLSAPTIQRMGASETMVRG
                     NVDSLVKLIAAFNEAGIEMIDEGAVSSAQGRGVRLKTDS"
     gene            544..1014
                     /locus_tag="VGA15_04240"
     CDS             544..1014
                     /locus_tag="VGA15_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586935.1"
                     /translation="MIPKTILTAAICAALLAGASPSKADQYRPDEFLSLDLSKVVLSP
                     KPLGPSSEFAPVAIEARTDPGSEGAGAQARAEQRANPRIAVSRNLKVTVPGAAVAVPN
                     VTVAHIRPEKPRGAVRAKLAQRHGNPLDAEARDTRIQVWPCQSGGICNWQRQND"
     gene            1145..2720
                     /locus_tag="VGA15_04245"
                     /pseudo
     CDS             1145..2720
                     /locus_tag="VGA15_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594742.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
     gene            complement(2863..2991)
                     /locus_tag="VGA15_04250"
     CDS             complement(2863..2991)
                     /locus_tag="VGA15_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586936.1"
                     /translation="MVKALRRWLEANELLAIVTGVAGALVVLSLLLVLGGYLIVTR"
     gene            3136..3954
                     /locus_tag="VGA15_04255"
     CDS             3136..3954
                     /locus_tag="VGA15_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9586937.1"
                     /translation="MEYLQSQGISLPRLGLGTFRMQGDVCRAAVESALGLGYRHIDTA
                     EMYGNEDAIGPAIAASGVARKDLHVTTKVWHENLAPDAIRRAFDTSLKKLRLDHIDLY
                     LVHWPAKNMNLPAIFETLLKLKEEGRARAIGVANFNLALLKTVVEEIKAPIACNQIEY
                     HVMLDQTPVRKYLAAKSIPLVAYCPLAQGRAASDATLMAIGRKHGASAAQVALKWLLD
                     QDGVAAIPKASRRESQKANLDALHVGLDDEDRQAIAALPKNQRFVSPGFAPAWD"
     gene            4266..>5009
                     /locus_tag="VGA15_04260"
     CDS             4266..>5009
                     /locus_tag="VGA15_04260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX9586938.1"
                     /translation="MRVIMRALMLLVAGQLSTHAQEAGLIEPHAGTWKTWVIASGTQF
                     RAPPPPDRTATEKELGELARIAATRDGAALDRVAYWDTGSPSYRWSEIAVAEHLRIGT
                     PWQIAARDLALMHIAIYDAMVAAWDSKYAYKRLRPSEVSADLTTVLPNPPSPSYPAEH
                     AVAAGAASEVLAYIFPERGEFFRKLADEAAGSRLIAGVNYASDVAAGMALGRQVAVLV
                     IARGKSDGTDAKWTGSVPTGPGKWTGTNPI"
BASE COUNT          979 a         1536 c         1538 g          956 t
ORIGIN      
        1 gaatcggttt tcagccgcac cccgcggccc tgcgcggagc tgacggcgcc ttcgtcgatc
       61 atctcgatcc cggcctcgtt gaacgcggcg atcaacttga ccagcgaatc gacattgccc
      121 cggaccatcg tttcgctggc ccccatgcgc tgaatggttg gcgcggaaag ccccgacagt
      181 tcggcgagcc gacgctgatc gatgccgaga agcaccctgg cagcccgcaa ttgtgccgct
      241 gtgatcatag gtctgccccc ggggacccta accaaacatc attcgtgcat gataactaat
      301 gatgttaaag tgtcaataag tgatatatga tatatctatt ctaaggggtt ccgtccacgc
      361 cgtcgccgca cggaacatcg cgccgaaacc atcacggcgc gcggagatgc cttcggggcc
      421 gctgatatcg acgcgcgcga atgttgttgg caactttggt ttgaccggca acttccgttt
      481 gaatttgcgc tcccggcttc ctacatgagg tgttgttctc ccaagcattt cagcggacct
      541 cacatgattc cgaaaacgat tttgacggcc gcgatttgcg ctgcgctgct ggcgggggct
      601 agcccgtcga aggcggatca gtatcgtccc gacgagttcc tcagcctcga cctttccaag
      661 gtggtgcttt cgccgaaacc gcttgggcct agttccgaat tcgcgccggt tgcgatcgag
      721 gcaagaaccg atcccggaag cgagggtgcg ggtgcgcagg cccgcgcgga acagagggcc
      781 aatcccagga tcgccgtttc caggaatctc aaggtcactg ttcccggggc cgcagtcgcg
      841 gttcccaacg tcacggtcgc gcatatccgc ccggagaaac cgcgcggcgc ggtccgagcc
      901 aagctcgcgc aacgtcacgg caatccgctc gatgcggaag cgcgcgatac caggattcag
      961 gtctggccgt gccagtcggg cggcatctgc aattggcagc ggcagaacga ttgagcagcg
     1021 cgtagccggg cccgcaacgc cgtcccgcgc aggtcgcttt cagtcatctg cctgtcgcaa
     1081 aaccgtcggc ccagagtcaa gttgagcggg caaccttcgt cgtgattcga cgagggggcc
     1141 ggccatggcg attgcgatac gcgcgaagca gggtttgacc agacgtcatc tgctggtccg
     1201 gtcagcatca acgctcgcgc tggcaggatt gggaagtcta gccaggcctt atctgagccg
     1261 ggcggcggat cgtccgctga tcgctaacgg actggcctca ggcgacgcat ccaccgactc
     1321 cgctgtggtg tgggcgaggg ccgaccggcc ggcgcgcatg cgggtggaat gctcaacgct
     1381 cgaaagtttc aagagcatac ttcgcgtcgc atcatcgaac gcgctgccgg atcacgattt
     1441 cacttcgaaa gtgctgctcg aaaatcttcc gaccgggcag gatattttct atcgggtgcg
     1501 gcttgaagac atcggtgaac ccgggatttc gggtgaaacc caggtcgggc atttccgcac
     1561 cgcgccgacg gacaaaagct cgatatcgcg atcttcgatc tcatttgtct ggtcgggcga
     1621 taccgcgggc cagggctggg gcatcgatcc ggcgcacggc ggcatgcgaa cctacaagac
     1681 catgctggaa agccgcccgg actttttcat ccattgcggc gatcacatct acgccgactg
     1741 tccgatccct tcagaattga aactgccaga tggcggtatc tggcgaaaca tcgtgaccga
     1801 ggaaaaatcc gctgtcgcgc acagccttaa gcagtttcgc ggcaattata aatacaacct
     1861 gctcgatggc aatttgcgcg ccttcaatgc gcaggtgccg atgctcgcgc aatgggacga
     1921 ccacgaagtc accaacgact ggtcgccggt cgggtccgcc gacgagaccg gttacgccga
     1981 ggacggcagc tccagactgg tggcgcgcgc ccgcagggcg tttttcgaat tcatgccgat
     2041 ccgcagcacg ccagcgcaag aggggcgtgt ctatcgccgg attggatacg ggccgctgct
     2101 cgacgtcttt ctgatcgaca tgcgcagcta tcgcgattcg acctggaaca aacgcgatga
     2161 ccgcagcgac accttcattc tgggctcggc gcagctggcg tggctgaaac gggagctcgc
     2221 tgcctctgat gcgacctgga aggtgattgc cgcggatctg ccgatcgggt tgatcagcga
     2281 ggacgcgatt gcgcttggcg acggaccacc ggagcggcgc gagcacgaaa ttgccgatct
     2341 gttgtcgttc atgaaacgcg ccggcgtgag aaataccgtg tggctgacgg cggacatgca
     2401 ctacacagcc gcgcaccatt acgatccgaa ccgcgcggtg tttcagcagt tcgagccgtt
     2461 ctgggaattc gtctccggtc cgcttcatgc ggggacatgg ggtccgggcg agctggataa
     2521 tacgttcggt ccgaaggcga tgtttcaaaa aggctgcagc gcggagcagg gcgaaaatct
     2581 cgccccgtgt ttcggactgc agttcttcgg ccgcgtcgag atcgacggcc aaggatgtcg
     2641 acaaccaaaa tctctggtcg gtcgatatcg agccgcggcc ggacgcgcgg ccgggaacca
     2701 tgctggctca gcatatctga gccgtctgga tcatgattcg attagattga atcggatcat
     2761 gatctcatct ctttgtttga gcatgatctc cgcgcaaacg cgttccgcgt ttgtcgcgag
     2821 ggaaaaccgg tatccacttt tccggatcat tgctccaaag cgtcagcgcg tcacgatcag
     2881 atagccgccc agcaccagca gcagggacag cacgaccagt gcgcccgcca cgccggtgac
     2941 gatcgccaaa agttcgttgg cctcgagcca tcgtcgtagt gccttgacca ttctaactcg
     3001 atcttgattt ggagcccgtg ctccgggcag actagcaaac ggcggacccg ctgtctcacc
     3061 tatcgcttcg aaacgcccta ttacgcggca tcgccggttg ctcatccggt gctttaagtc
     3121 tttaaggagt tcatcatgga atatctgcaa tcccagggca tcagcctgcc gcggcttggg
     3181 ctcggcacct ttcgcatgca gggtgatgtc tgccgcgcgg cggtcgaaag cgcgctcggt
     3241 ctcggctatc ggcatatcga caccgccgaa atgtatggca acgaagacgc catcgggccc
     3301 gccatcgccg catccggcgt cgcgcgcaag gatctgcacg tcaccaccaa ggtctggcat
     3361 gaaaatctcg cgccggatgc gatccgccgg gcgttcgaca ccagcctgaa aaagctgagg
     3421 ctcgatcata tcgatctcta tctggtgcac tggccggcga agaacatgaa cctgccggca
     3481 atcttcgaaa ccttgctgaa gctgaaagaa gagggccgcg cccgtgccat cggcgtcgcc
     3541 aatttcaatc tcgcgctcct gaaaaccgtg gtcgaggaga tcaaggcccc gatcgcctgc
     3601 aaccagatcg aataccatgt gatgctcgac cagacgccgg tgcggaaata tcttgccgcc
     3661 aaatcgatcc cgctggtggc ctattgcccg ctggcgcagg gccgggcggc ctccgacgcg
     3721 acgttgatgg cgatcggtcg caagcatggc gccagcgcgg cgcaggtggc gttgaaatgg
     3781 ctgctcgatc aggatggggt cgccgcgatc ccgaaggcgt cgcgccgcga gagccaaaag
     3841 gccaatctcg acgcgctgca tgtcgggctc gacgatgagg accgacaggc catcgccgcg
     3901 ctgccgaaaa accagcgctt tgtcagtccc ggttttgcgc cggcgtggga ttaggccgtg
     3961 aactcatgaa tgcgccgatc ggactgactg cccgagtccg ctttgtcccc gaagccgacc
     4021 aatcgttgca tcgcagcgaa acgatgcgat ctgccacaac cggaaatcgc gaccctttca
     4081 gcggtccggg aatgagaata taattggaac tgcgcttggc ttgctccggt gtggttgctg
     4141 cattcgatcc tgttgccgtc gacgcatggc aactctggcc gtgcgatctg gcaacagcaa
     4201 ccgtcatcta gaccagttca cgaatcgatt ttttaccgac cccacaacga caagggagga
     4261 ttgctatgcg ggtcatcatg cgcgcgctga tgctgctcgt tgcgggccag ctttccacgc
     4321 atgctcagga agcggggctc atcgagccac atgccggaac ctggaagacc tgggtgatcg
     4381 cctcgggcac acaattccgc gcgccgccgc cgcccgatcg cactgccacc gagaaggagc
     4441 tcggcgagct cgcgcggatc gcggcaacgc gcgatggcgc cgcgctcgat cgtgtcgcct
     4501 attgggacac gggctcgccg tcctatcgct ggagcgagat tgccgtcgcc gagcacctca
     4561 ggatcgggac tccctggcag attgcagcac gcgatctggc gctgatgcac atcgcaatct
     4621 acgacgccat ggttgcggct tgggacagca aatacgctta caagcggcta cgtccgagcg
     4681 aagttagcgc cgatctcacg accgtgttgc cgaatccgcc gagcccctct tatccggccg
     4741 agcatgcggt cgcggcgggc gcggcatcgg aggtcctggc ttatattttt ccggagcgcg
     4801 gcgaattctt ccggaagttg gccgatgagg cggccggttc gcggctgatc gcgggcgtca
     4861 actacgcaag cgatgtcgcg gcaggcatgg cgctcggcag gcaggtcgcc gtactcgtga
     4921 tcgcgcgcgg aaagtcggac ggaacggacg ccaaatggac cggttcggtg ccgaccggcc
     4981 ccggcaagtg gaccggcacg aacccgatt
//
LOCUS       DASWGM010000141         3447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2229680,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000141 DASWGM010000000
VERSION     DASWGM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2229680"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGA15_04265"
     CDS             complement(<1..369)
                     /locus_tag="VGA15_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9586939.1"
                     /translation="MSILIDKNTKVICQGFTGKNGTFHSEAAIAYGTRMVGGTSPGKG
                     GSTHLDLPVFDTVQEAKEKTGADASVIYVPPPGAADAICEAIDAEIPLIVCITEGIPV
                     LDMVRVKRALSGSKSRLIGPN"
     gene            complement(406..885)
                     /locus_tag="VGA15_04270"
     CDS             complement(406..885)
                     /locus_tag="VGA15_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX9586940.1"
                     /translation="MAQDRLTAPTALEEHRKLAAFAGEWNGEEVVQPSRWTAGGPATS
                     RVVARMDLNGFYLIQDTRQMRDGKESFATHGVFTYDREDRHYKLFWHDSLGYYSPAPA
                     SGGWTGKTLILVRGSLRGNARHVYEILDDNTYSMKIQFSPDAEGWADVLTGVYRRIN"
     gene            complement(890..2086)
                     /gene="sucC"
                     /locus_tag="VGA15_04275"
     CDS             complement(890..2086)
                     /gene="sucC"
                     /locus_tag="VGA15_04275"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX9586941.1"
                     /translation="MNIHEYQAKALLHEFGVPISRGAAVLRASDSDAAAKALGGPVWV
                     VKSQIHAGGRGKGKFKEASAGDKGGVRIAKSVAEVNEFAKQMLGATLVTVQTGSHGKQ
                     VNRLYVEEGSDIDKEFYLSILVNRETSKISFVVSTEGGVNIEDVAHSTPEKIVTFSVD
                     PATGIMAHHGRIVAKALGLTGDLAKQAERLATQLYTAFVAKDMAMLEINPLVVTKQGD
                     LRVLDAKVSFDDNALYRHPDVLALRDETEEDAKEIEASKHDLNYVTLDGNIGCMVNGA
                     GLAMATMDIIKLYGMAPANFLDVGGGADRAKVTAAFKIITADPNVKGILVNIFGGIMK
                     CDIIAEGVVAAVKEAGLKVPLVVRLEGTNVDAGKKILRESGLNVVPADDLDDAAQKIV
                     KAVKGS"
     gene            complement(2237..3205)
                     /gene="mdh"
                     /locus_tag="VGA15_04280"
     CDS             complement(2237..3205)
                     /gene="mdh"
                     /locus_tag="VGA15_04280"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX9586942.1"
                     /translation="MARDKIALIGSGQIGGTLAHLIGLKELGDVVLFDIAEGVPQGKA
                     LDIAQSSPVDGFDAHFTGANSYEALDNAKVCIVTAGVPRKPGMSRDDLLSINLKVMEQ
                     VGAGIKKYAPDAFVICITNPLDAMVWALQKASGMPHKKVVGMAGVLDSSRFRYFLADE
                     FNVSVEDVTAFVLGGHGDTMVPLVRYSTVAGIPLPDLVKMGWTSQARIDEIVDRTRNG
                     GAEIVNLLKTGSAFYAPAASAIAMAESYLKDKKRVLPCAAYLSGEYGVKDMYVGVPVV
                     IGSKGVERVVEIELAGKDREAFDKSVGAVQGLVEACKKIAPDLLGR"
BASE COUNT          623 a         1075 c         1047 g          702 t
ORIGIN      
        1 attcggtccg atcagccgcg atttcgatcc ggagagcgca cgtttcaccc gcaccatgtc
       61 gagcaccgga atgccctcgg tgatgcagac gatcagcggg atttcggcat cgatggcttc
      121 gcagatcgca tctgccgcgc cgggcggcgg cacatagatc acgctggcat cggcgccggt
      181 cttttccttt gcctcctgca ctgtgtcgaa caccggcaag tcgagatggg tagagccgcc
      241 tttgcccggc gaggtgccgc cgaccatcct ggtgccatag gcgatcgccg cttccgaatg
      301 aaacgtgccg ttcttgccgg taaagccctg gcaaatgact ttcgtattct tgtcgatcag
      361 gatggacatg tctgggattg ctttcgcgaa acgaacagta agggctcagt tgatccgccg
      421 gtagacgccg gtgagcacgt cagcccatcc ttccgcgtcg ggcgaaaact ggattttcat
      481 gctgtaggtg ttgtcgtcga ggatttcata gacatggcgc gcattgccgc gcagcgagcc
      541 gcgcaccagg atcagcgtct tgccggtcca gcctccggac gccggcgccg gcgagtagta
      601 gccgagtgaa tcatgccaga acaatttgta gtgccggtcc tcgcggtcat aggtgaacac
      661 gccatgggta gcgaagcttt ccttaccgtc gcgcatctga cgcgtgtcct gaatcagata
      721 aaagccgttg agatccatgc gcgcgacgac gcgcgaggtg gcgggccctc ccgcggtcca
      781 gcgcgacggc tgcaccacct cttcaccgtt ccactcaccc gcgaaggcgg cgagtttgcg
      841 atgctcctcg agcgccgtcg gcgccgtgag acggtcctgg gccattgtct tagctcccct
      901 tcacagcctt gacgattttc tgcgcggcat cgtcgagatc atcggccggc accacgttga
      961 gaccggattc ccggaggatt ttcttgccgg cgtcgacatt ggtgccctcg agccgcacca
     1021 ccagcggcac tttcaggccg gcttccttca ccgccgcgac cacgccctcg gcgatgatat
     1081 cgcatttcat gatgccgccg aagatattga ccaggatgcc cttcacattg ggatcggcgg
     1141 tgatgatctt gaacgccgcg gtgaccttgg ccctgtcggc gccgccgccg acgtcgagga
     1201 agttcgccgg cgccatgccg tagagcttga tgatgtccat cgtcgccatc gccaagccgg
     1261 cgccgttgac catgcagccg atgttgccgt cgagggtgac gtaattgaga tcgtgtttcg
     1321 acgcctcgat ttccttggcg tcctcctcgg tctcgtcgcg cagcgccaac acgtcggggt
     1381 ggcgataaag cgcattgtcg tcgaacgaca ccttggcgtc gagcacacgc agatcgccct
     1441 gtttggtgac gacgagcgga ttgatttcca gcatcgccat gtccttggcg acgaaggctg
     1501 tgtagagctg cgtcgccagc cgctcggcct gtttggcaag atcgccggta agacccagcg
     1561 ccttggcgac aatgcggccg tggtgggcca tgatgccggt ggcgggatcg acggagaagg
     1621 tcacgatctt ctccggcgta gagtgcgcga cgtcctcgat attgacgccg ccctcggtgg
     1681 agaccacgaa cgatattttg gaggtctcgc ggttgaccag gatcgacaga taaaactcct
     1741 tgtcgatgtc ggagccttcc tcgacataga gccgattgac ctgcttgccg tgcgatccgg
     1801 tctgcaccgt gaccagcgtc gcccctagca tctgcttggc gaattcattg acctcggcaa
     1861 ccgatttggc gatgcgcacg ccgcccttgt cgccggcgga tgcttccttg aacttgccct
     1921 tgccacggcc gccggcgtgg atctggctct tcaccaccca gaccggacca ccgagcgctt
     1981 ttgcggcagc gtcggaatcg gaggcgcgca gcaccgccgc gccgcgcgag atcggcacgc
     2041 cgaattcatg cagcagtgcc ttggcctggt attcatgaat gttcatttgg acgttcccag
     2101 gctcctcgtg cggtgaattc ggagtttcac atcaggtttt gcggctggca taccatatac
     2161 cagaaaaact gcaaatttgg aaattggagt gtcgaaagtc gattggcttg atctatcggg
     2221 tgatagccaa gcgcaatcag cggcctagca ggtcgggcgc gatcttcttg caggcctcga
     2281 ccagaccctg cacggcgccg accgacttgt cgaacgcttc gcggtccttg ccggccagtt
     2341 cgatctcgac gacgcgctcg acgcctttcg atccgatcac gacgggcacg ccgacataca
     2401 tgtccttcac gccgtattcg cccgaaagat aggcggcgca gggcagcacg cgcttcttgt
     2461 ccttgaggta gctctcggcc atcgcaatcg cggatgcggc cggcgcatag aacgccgaac
     2521 cggtcttcaa caggttgacg atctcggccc cgccgttgcg ggtgcggtcg acgatctcgt
     2581 cgatccgcgc ctgcgaggtc cagcccatct tgaccagatc cggcagcgga atgccggcca
     2641 cggtagagta tctcaccagc ggcaccatgg tgtcgccgtg gccgccaagc acgaaggcgg
     2701 tgacgtcttc gaccgagacg ttgaactcgt cggcaaggaa atagcggaag cgcgaggaat
     2761 ccagcacgcc cgccatgccc actaccttct tgtgcggcat gcctgatgcc ttctgtagcg
     2821 cccacaccat ggcgtcgagc gggttggtga tacagatcac gaaggcatcg ggcgcatatt
     2881 tcttgatgcc ggcgccgacc tgctccatca ccttgagatt gatgctcaag agatcgtcgc
     2941 ggctcatgcc gggcttgcgc ggcacgccgg cggtgacaat gcagaccttg gcattgtcga
     3001 gcgcctcata ggaattagcg ccggtgaaat gcgcgtcgaa cccgtcgacc ggggaggatt
     3061 gcgcgatgtc gagcgctttg ccctgcggca cgccctcggc gatgtcgaac aacaccacgt
     3121 cgcccagttc cttcaggccg atcaggtgag ccagcgttcc gccgatctgg ccggagccaa
     3181 tcaaagcaat cttgtcgcgc gccatgggaa atctgtcctt tgaaggtcaa catgagggtg
     3241 gaaggaagct gacaggctgg ttatcccttt cgaccgaccc gttcaagtcg cgccgtgctc
     3301 aattgtcggc cactcgcctc attccggggc gcgtcgaaga cgcgagcccg gaatgaccgc
     3361 gacgacggat tcacgtctcg acaagtccgg tgctcgagcc gcttgcgacg gaatccttgt
     3421 ggccgtgcgg cagcgccaga taggatt
//
LOCUS       DASWGM010000142         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1256354,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000142 DASWGM010000000
VERSION     DASWGM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256354"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VGA15_04285"
     CDS             complement(<1..945)
                     /locus_tag="VGA15_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9586943.1"
                     /translation="MDSVVTAPRSEADERFDSARIAAAVDALAAKHAGREDLFRAAVA
                     QLLKAEMTAARAVAQEVLLRDRHGRRCAERLCFVQDEIIRILYAAATRHLYHSPIPSG
                     AERMAVVATGGYGRGLMAPESDIDLLFILPYKQTAWGEQVAEAILYCLWDMGLKVGHA
                     TRSVDESIRQARGDMTIRTAILETRFLTGDQPLYNELVARFDKEVVQGSAAEFVTAKL
                     AEREERHRRAGQSRYLVEPNVKDGKGGLRDLHTLFWIAKYVYSVRKTDELLERGVFDA
                     QEYRTFRRCADFLWSVRCNIHFFSGRAEERLSFDMQREI"
     gene            complement(978..1184)
                     /locus_tag="VGA15_04290"
     CDS             complement(978..1184)
                     /locus_tag="VGA15_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586944.1"
                     /translation="MIKTDRKKASTQGIAPPSPAQETPFHFNELNELVNEKIRTSNRS
                     GSEQLCSKRSEYSMDLLLPYGMFP"
     gene            1208..>1759
                     /locus_tag="VGA15_04295"
     CDS             1208..>1759
                     /locus_tag="VGA15_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586945.1"
                     /translation="MFKISRRVVAAIIAVSALLPGGAYAADALKEIHIDWATYNPVSM
                     VLKQKGLLEKEFAKDGIGIGIVWVQSAGSNKALEFLNAGSIDFGSTAGSAALVARING
                     NPIKSIYVFSRPEWTALVTARDSRIASVADLKGKRVAVTRGTDPHIFLVRALLDAGLS
                     EKDITPVLLQHADGKTALIRGDVD"
BASE COUNT          323 a          537 c          553 g          346 t
ORIGIN      
        1 gatttcgcgc tgcatgtcga atgacagccg ctcttcggcg cggcccgaga agaaatggat
       61 gttgcagcgg accgaccaca ggaaatcggc gcagcggcgg aaggtgcggt attcctgcgc
      121 gtcgaacacg ccgcgttcca atagctcatc ggtcttgcgc acgctgtaga catatttcgc
      181 gatccagaac agcgtatgca gatcgcgcaa tccgcccttg ccgtccttga cgttgggctc
      241 gaccagatag cgcgattgcc cggcgcggcg atgccgttcc tcgcgctcgg cgagtttcgc
      301 ggtgacgaat tcggcggcgg agccctgcac cacctccttg tcgaaccgcg ccaccagttc
      361 gttatagagc ggctggtccc cggtcagaaa tcgcgtctcg aggatcgcgg tgcggatcgt
      421 catgtcgccg cgcgcctgcc ggatcgattc atcgaccgag cgggtggcat ggccgacctt
      481 caggcccatg tcccagagac aataaagaat ggcttcggcg acctgctcgc cccaggcggt
      541 ttgcttgtag ggcaggatga acaacagatc gatatcggat tccggcgcca tcagcccgcg
      601 gccatagccg ccggtggcga cgaccgccat gcgctcggcg cccgagggga tcggcgagtg
      661 atagagatga cgcgtggcgg cggcgtacag gatgcggatg atttcatcct ggacgaaaca
      721 caagcgttcg gcgcagcgcc gtccgtgacg atcccgcagc agcacctcct gcgccaccgc
      781 gcgggccgcg gtcatttcgg ctttcagcaa ctgcgccacg gcggcgcgaa acaggtcctc
      841 gcgtccggcg tgtttcgcag cgagcgcgtc gaccgcggcg gcgatccgcg cgctgtcgaa
      901 acgttcgtcg gcctcggatc ggggtgctgt cacgacgcta tccatcccta tcccgatatc
      961 ggacggcgcg aacgctgtca cgggaacatt ccgtaaggta acagcagatc catgctgtat
     1021 tctgaacgct tcgagcaaag ctgttctgat ccggacctgt tcgacgtacg tattttctcg
     1081 ttgacgagtt catttaactc attgaaatga aaaggcgttt cttgagcggg ggagggcggc
     1141 gcgatccctt gcgtggacgc tttcttgcga tctgttttga tcatccactc ccatggagat
     1201 aacgacgatg ttcaagattt cacggcgcgt tgtggccgcg atcattgccg tctcggcgct
     1261 gttgcccggt ggcgcttacg ccgccgacgc gctcaaggaa attcatatcg attgggcgac
     1321 atataacccg gtgtcgatgg tcctcaagca aaagggattg ctcgagaagg aattcgccaa
     1381 ggacggcatc ggcatcggca tcgtctgggt gcaatcggcc ggttccaaca aggcgctcga
     1441 gttcctcaat gccggctcga tcgatttcgg ctcgaccgcg ggctccgcgg cgctggtcgc
     1501 gagaatcaac ggcaatccga tcaagtcgat ctatgtcttt tcgcggccgg aatggacggc
     1561 tttggtgacg gccagggatt ccaggatcgc tagcgtcgcc gacctcaagg gcaagcgcgt
     1621 cgcggtcacc cgcggaaccg atccgcacat cttcctggtg cgcgcgctgc tcgatgcggg
     1681 cttgtccgag aaggacatca cgccggtgct gctgcagcac gccgacggca agaccgcgct
     1741 gatccgcggc gatgtcgat
//
LOCUS       DASWGM010000143         9014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1256458,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000143 DASWGM010000000
VERSION     DASWGM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9014
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256458"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGA15_04300"
     CDS             <1..286
                     /locus_tag="VGA15_04300"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9586946.1"
                     /translation="PNYRYNPDYKFKGVKSKEAFTIKPGPNNPAGSYWIGLSAEGYGI
                     HGTADPSRVSKSESHGCIRLTNWDVGLLGRSTKRGTPVAFIDEPQGGRKS"
     gene            complement(358..570)
                     /locus_tag="VGA15_04305"
     CDS             complement(358..570)
                     /locus_tag="VGA15_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586947.1"
                     /translation="MSVPHIPGMTDEELMVYAMREAQSVLAQHIERSPQDAEETIARL
                     LEILDRQDVVNATDRLCRGYGLRPIR"
     gene            complement(603..1412)
                     /locus_tag="VGA15_04310"
     CDS             complement(603..1412)
                     /locus_tag="VGA15_04310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9586948.1"
                     /translation="MTRIALVVSDVDGTLLTKDKTLTDGARRAVRRLHEAGIGFTITS
                     SRPTIGMGFLIEPLQITLPVGPFNGSSIVDPQLNPLEQHLIPAAAAQTSLDLLNQFGV
                     DIWLFTNEKWLTRRPDGEYVPHEKLAIRADPTIVTDFTPYLAAACKIVGASSDADLLK
                     RCEAAMQRAVGTQATAIRSQSYYLDVTPPGFDKGTFVQAMAKRQGMSTDAVATIGDMQ
                     NDLAMFAKSGMSIAMGNATEDVKKRATHVTTSNQDEGFAGAIDLILKNNAS"
     gene            complement(1409..1927)
                     /locus_tag="VGA15_04315"
     CDS             complement(1409..1927)
                     /locus_tag="VGA15_04315"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEX9586949.1"
                     /translation="MGVSGSGKSTIADKLAERLGWTYEDGDKFHPASNVAKMSAGHPL
                     TDEDRWPWLQAIAGEIDRVCKAGQHVVIACSALKHAYRDILVHGRDDVRIVYLKGTPK
                     LIGDRLAQRKGHFMPPGLLASQFKTLEPPDASENPVTVSIDASVETIVDDIVRQLNPD
                     PADSAAPRRNRS"
     gene            complement(1959..2705)
                     /gene="pgl"
                     /locus_tag="VGA15_04320"
     CDS             complement(1959..2705)
                     /gene="pgl"
                     /locus_tag="VGA15_04320"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX9586950.1"
                     /translation="MAANDPHHVITVSDAAALAKAAAERVMARIAANSGRVAICLTGG
                     SSPKQLYHLLATDAYRSRIPWERVHWFIGDERFVPADDPLHNMAVARRIFLDRWAPDS
                     NIHPIPTDTADPREAARRYESELKSFYGANELVSSRPLFDLVLMGVGPDGHTASLFPD
                     YPAVEEIERWVVGVPQAHVEPFVPRVTLTLPALDSCREMLFEVSGPDKRAILTRVLDG
                     ENLPANRVRATGETIWLADEAALPENFRER"
     gene            complement(2710..4242)
                     /gene="zwf"
                     /locus_tag="VGA15_04325"
     CDS             complement(2710..4242)
                     /gene="zwf"
                     /locus_tag="VGA15_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX9586951.1"
                     /translation="MTDVSSNQNRRTNQKKPDPCSFVIFGVTGDLTHRLVIPSLYNLA
                     ATDLLPDKFCVVGIARKGTSNDALRDSLMKGLRTYATRPVDDTVAQRLLECVTSIEAD
                     PRDPASFDAMRERLEKLEANRSTGGNRLFYLATPPDAFAPISRELGRTGLLREENGAW
                     RRLVIEKPFGTDLASAKALNGELLKIVDEHQLYRIDHYLGKETVQNILVLRFANGMFE
                     PIWNRNHIDHVQITVDEKLGVGHRGSFYDSTGALRDMVPNHLFQLLSLVAMEPPIRFD
                     AHSVRSEKADVLAAIQTQSEADALKNSVRGQYQGGKIGDTEIEDYCKTEDVKPGSTTE
                     TYAALKLTIDNWRWAGVPFYLRTGKALGIKRTEVAIKFKQAPFAMFSCTEVDRLSQNY
                     LVISIEPTEGITLQFNTKVPGPTINIDGVEMKFRYKDYFKAEPATGYETLIYDCMIGD
                     NILFQRADSVEAGWQAVQPFLDAWKEAGANGLQSYKAGSEGPADADALLARDGRSWRK
                     LG"
     gene            complement(4239..5276)
                     /gene="gnd"
                     /locus_tag="VGA15_04330"
     CDS             complement(4239..5276)
                     /gene="gnd"
                     /locus_tag="VGA15_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX9586952.1"
                     /translation="MQLGMIGLGRMGGNIVRRLMKHGHTTVVYDKDPKAVAALAADGA
                     AGSGALEDFVSKLEKPRTAWVMLPAGKITEATIDALSKLMQAGDVIIDGGNTFWQDDV
                     RRGKALKEHGIHYIDVGTSGGVWGIDRGYCMMIGGDKAVVDRLDPIFASLAPGIGDIE
                     RTGGREGRDPRIEQGYIHAGPVGAGHFVKMVHNGIEYGLMQAYAEGFDILKNANINAL
                     PAEHRFDLDIADIAEVWRRGSVIPSWLLDLTASALARNDLLDNYSGFVEDSGEGRWTV
                     NAAIDEAVPAEVLTAALFARFRSRKEHTFAEKILSAMRDGFGGHKEPQQHPDPARQNA
                     PDIVKPKADRP"
     gene            complement(5496..8342)
                     /locus_tag="VGA15_04335"
     CDS             complement(5496..8342)
                     /locus_tag="VGA15_04335"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEX9586953.1"
                     /translation="MNPVKALENHGQAVWLDFLARSFVAKGDLKKLIDTDGVKGVTSN
                     PSIFEKAIGSSDEYDGAIEKALKKGDRPIAELFEHLAVEDIQHAADVLRPVYDQLKGE
                     DGFVSLEVSPYLAMDTKATIAEAERLWKDVKRKNLMVKVPATPEGLPAIQHLIGEGIS
                     INITLLFSQKVYVQVAQAYLAGLEKYVAKGGDPSHVASVASFFVSRIDSAVDKALDEK
                     IARANDPTEKERLAALKGKIAIANARLAYQDYERLFSGTRWDKLAAKGAKPQRLLWAS
                     TGTKNKDYSDVLYVEELIGPNTVNTVPPATLDAFRDHGTPRDSLEENIEDARGVLAEL
                     ETSGISLDAITSDLVKDGVKLFADAADKLYGAVAHKRAAVLGAGIDRQEFALGSSLGK
                     ALEKSTEDWRASGKIRRLWQHDKSVWTGTDEDKWLGWLNSAAAADIADYEDFAHRVKG
                     QNFTDAVVLGMGGSSLGPEVLAQTFEKQPGFPKLHVLDSTDPAQVRKLQASINIANTL
                     FIVSSKSGGTTEPNVMKDYFFARVSETIGPDKAGHRFIAVTDPGSSLEKIATKQGFAR
                     IFHGNPSIGGRYSVLSPFGLVPAATAGIDIATLLKHTLAMVRSCGPDVPPHENPGVQL
                     GLAMGLAGLEGRDKVTLLSSKKIADFGAWAEQLIAESTGKDGKGLIPIDSEPLGDVAI
                     YGNDRFFIDIRTEGENDLAHDDRLAALEKAGHPLVRIVMKSIEHIGQEFFRFELATAV
                     AGAVLGINPFNQPDVEDAKIKTRELTAAFEKSGALPAEQPVISTAQADLYTDDKNAAA
                     LRKAGADGDIGSWLKAHLARAGADDYVALLAYIERDSAHIDALQQMRLEVRDKRHVAT
                     CAEFGPRFLHSTGQAYKGGPDSGVFLQITADDDKDLSVPGQKASFGVIKAAQARGDFD
                     VLTERGRRALRVHLKGDLDTGLKLLDTAIQDALN"
BASE COUNT         1635 a         2731 c         2785 g         1863 t
ORIGIN      
        1 tccgaactat cgctataatc ctgattacaa attcaaaggc gtcaaatcca aagaggcttt
       61 caccatcaag ccggggccga ataacccggc cggatcctat tggatcgggc tctcggcgga
      121 aggatatggc atccatggca cggctgaccc ttccagggtc agcaaatccg aatcgcacgg
      181 ctgcattcgg ctgacgaatt gggacgtcgg tttgttgggc cgcagcacca aaaggggaac
      241 gccggtggcg ttcatcgacg aaccgcaagg cggccgaaag agctgaccga cgcaggcgcg
      301 cgcagacgag ggcgaatgat cacgcagtta tcttgtggcc ggtacctctg agcaaaatca
      361 acgtatcggc cgcagcccat agccacggca caggcggtcg gtcgcgttaa cgacgtcctg
      421 acgatcgaga atttccagaa gccgcgcgat cgtttcctca gcgtcttgcg ggctccgctc
      481 gatgtgctgg gcaagaaccg attgggcttc gcgcatcgcg tagaccatca gctcttcgtc
      541 ggtcatgccg gggatgtgag ggacgctcat gttcgctttc tgtccttggg cggcgatccg
      601 gactagctcg cgttgttttt caaaataaga tcgatcgcgc cggcaaagcc ttcgtcctgg
      661 tttgatgtcg tcacatgcgt cgcccgtttc ttgacgtcct cggtggcgtt gcccatcgcg
      721 atcgacatgc cgctctttgc gaacatcgcc agatcgttct gcatgtcgcc gatggtggcg
      781 accgcatcgg tcgacatgcc ctgacgcttg gccatcgcct gcacgaaggt gcctttgtcg
      841 aagcccggtg gcgtgacgtc gagataatag ctttgcgacc ggatggccgt ggcctgcgtc
      901 cccacggccc gctgcatcgc ggcttcgcag cgtttcagca ggtcagcgtc agagctggcg
      961 ccgacgattt tgcaggcggc cgccaaatag ggcgtgaaat ccgtaacgat ggtcggatcg
     1021 gccctgatcg caagtttctc atgcggaacg tattcaccgt cgggccggcg cgtgagccat
     1081 ttttcgttgg taaacagcca gatatcgaca ccgaattggt tgagcagatc gagactggtt
     1141 tgcgccgcgg ccgcggggat cagatgttgt tcgagcgggt tgagttgcgg gtcgacgatc
     1201 gaacttccat tgaagggccc gaccggcaag gtaatctgca gcggttcgat caaaaacccc
     1261 atgccgatgg tcggacggct ggaggtaatg gtgaaaccga tgccggcctc atgcagccgc
     1321 cgcacagcgc gcctggcgcc atcggtcaag gtcttgtcct tggtcaatag cgtgccgtcg
     1381 acatcggaga cgaccagggc gatgcgggtc atgagcggtt cctgcgcggc gcggcgctgt
     1441 cagcgggatc tggattcagt tggcgaacga tatcatcgac gatagtctcg accgacgcgt
     1501 caatcgacac ggtcacagga ttttcgctgg cgtctggcgg ctcgagcgtc ttgaactggc
     1561 tggccagcag tcccggcggc atgaaatggc ccttccgttg ggcgaggcgg tcgccgatca
     1621 attttggcgt acccttgaga tagacgatcc ggacatcgtc ccgtccatgc acgaggatgt
     1681 cccgataggc gtgcttcagc gcggagcagg cgatcaccac atgctggccg gccttgcaga
     1741 cccggtcgat ctcgccggca atcgcctgca gccacggcca gcggtcttca tcggtcaagg
     1801 gatggccggc gctcatcttg gcgacgttgc tcgcggggtg aaacttgtcg ccatcctcgt
     1861 aggtccagcc gagccgttcg gcgagcttgt ccgcgatggt gctcttgccc gagccggaca
     1921 cgcccatcac gaccagcgcg caggggcttt cgcctgtctc atcgctcacg gaaattctcc
     1981 ggcagcgcag cctcatccgc cagccaaatc gtctcgccgg tggctcgcac acggttggcg
     2041 ggcaggttct cgccgtccag cacgcgcgtc aagatcgcgc gcttatcggg gccagacacc
     2101 tcgaacagca tctcgcggca ggagtcgagc gccggcaaag tcagcgtcac ccgcggcacg
     2161 aatggctcga catgtgcttg gggcacgccg accacccagc gctcgatttc ctcgacggcc
     2221 ggataatccg gaaacagcga tgcggtatgg ccgtcgggac cgacccccat cagcaccaga
     2281 tcgaacaacg gccgggaaga aaccagctcg ttcgcgccgt agaacgattt caactcgctt
     2341 tcgtaacgcc gtgcggcctc ccgggggtcg gcggtgtcgg tcggaattgg gtggatgttg
     2401 gaatccggcg cccagcgatc cagaaagatc cgccgcgcca cggccatgtt atggagcgga
     2461 tcgtccgccg ggacaaaacg ctcatcgccg atgaaccagt gcacgcgttc ccacgggatc
     2521 cggcttcgat aggcgtcggt cgcgagcaaa tgatagagct gtttcgggct cgatccgccg
     2581 gtcaggcaaa tcgccacgcg accgctattg gcggcaatcc ttgccatgac acgttccgcg
     2641 gcggctttgg caagcgctgc ggcatcggac accgtgatca catggtgagg atcgttcgct
     2701 gccatccgac taaccgagct tgcgccagct gcggccgtcg cgcgccagca gcgcgtcggc
     2761 gtccgccgga ccttcgctgc cggccttgta actttgcagg ccgttggcgc ccgcctcctt
     2821 ccacgcatcg aggaacggct gcaccgcctg ccagccggct tcgacgctgt cggcgcgctg
     2881 aaacaggatg ttgtcgccga tcatgcagtc atagatcagc gtctcatagc cggtggccgg
     2941 ctcggccttg aagtagtcct tgtagcggaa tttcatctcg acgccgtcga tgttgatcgt
     3001 cggtcccggc accttggtgt tgaactgcag cgtaatgcct tcggtcggct cgatgctgat
     3061 gacaaggtag ttctgcgaca ggcgatctac ctcggtgcaa ctgaacatcg cgaacggcgc
     3121 ctgcttgaac ttgatcgcca cttcggtgcg cttgatgccg agcgccttgc cggttcgcag
     3181 ataaaaggga accccggccc agcgccaatt gtcgatggtc agtttcagcg cggcataggt
     3241 ttccgtagtg ctgccgggct tgacgtcctc ggtcttgcaa taatcctcga tctcggtgtc
     3301 gccgatcttg ccgccctgat actggccgcg caccgaattc ttcagagcgt ccgcttcgct
     3361 ctgggtctgg atcgccgcca gcacatcggc cttttcggag cgcaccgaat gcgcgtcgaa
     3421 ccggatcggc ggctccatcg cgaccaatga cagcagttga aacaagtggt tcggcaccat
     3481 gtcgcgcagc gcgccggtcg agtcgtagaa actgccgcgg tggccgacgc cgagcttctc
     3541 gtcgacggtg atctgcacgt gatcgatgtg attgcggttc cagatcggtt caaacatgcc
     3601 gttggcaaag cgcagcacca ggatgttctg caccgtctcc ttgccgagat aatgatcgat
     3661 gcgatagagc tggtgctcgt cgacaatttt gagcaattcg ccgttcagcg ctttcgccga
     3721 ggcgagatcg gttccgaacg gtttttcgat caccagccgc cgccacgcgc cgttttcttc
     3781 cctgagcagg ccggtgcggc cgagttcccg gctgatcggc gcgaaggcat cgggcggtgt
     3841 cgccagataa aacagacggt tgccgccggt cgatcggttt gcttccagtt tctccagtcg
     3901 ctcccgcatg gcatcgaacg aggccgggtc cctcgggtcg gcttcaatgc tggtgacgca
     3961 ttccagcaga cgttgggcga ccgtatcgtc gaccgggcgg gtggcgtagg tgcggaggcc
     4021 cttcatcagg ctgtcgcgaa gggcgtcgtt cgacgtgccc ttgcgagcga tgccgaccac
     4081 acagaacttg tcgggcagta gatcggtggc cgccagatta taaagcgaag gaatgaccag
     4141 ccggtgcgtc aggtcgccgg tgacgccgaa aatgacgaag gagcagggat caggcttctt
     4201 ctgatttgtg cgtcgattct gatttgaaga gacgtcggtc acggacggtc ggcctttggc
     4261 ttgacgatat cgggcgcatt ctgccgcgcc ggatcggggt gctgctgcgg ttccttatgg
     4321 ccgccaaaac catcgcgcat cgccgaaaga atcttttcgg cgaaggtatg ttctttgcgc
     4381 gagcggaagc gtgcgaacag cgcagcagtg agcacctcgg cgggcacggc ctcgtcgatg
     4441 gcggcgttga cggtccagcg gccttcgccg gaatcctcga cgaaaccgga gtaattatcc
     4501 agcaaatcgt tccttgcgag cgcggaagcg gtgagatcga gcagccatga cgggatcacg
     4561 ctgccgcgcc gccagacttc ggcgatatcg gcaatgtcga gatcgaagcg atgctccgcg
     4621 ggcagcgcgt tgatattggc attcttgaga atgtcgaatc cctcggcata ggcctgcatc
     4681 aggccatatt cgataccgtt atgaaccatc ttgacgaagt gcccggcgcc gaccgggccg
     4741 gcatggatat agccttgctc gatacgcgga tcgcgtccct cgcgcccgcc ggtgcgctcg
     4801 atatcgccga tgcccggcgc caggcttgcg aagatcggat cgaggcgatc caccaccgcc
     4861 ttgtcgccgc cgatcatcat gcaatagccg cggtcgatac cccacacgcc gcctgacgtg
     4921 ccgacgtcga tgtagtggat gccatgctcc ttcagcgcct tgccacggcg gacgtcgtcc
     4981 tgccagaacg tgttgccgcc atcgatgatg acgtcgccgg cctgcatcag tttcgacagc
     5041 gcgtcgatgg tcgcttcggt gatcttgccc gccggcagca tcacccaggc ggttcgcggc
     5101 ttctcaagct tgctgacgaa atcttccagc gcgcctgagc cggcagcacc gtcggccgcg
     5161 agcgctgcca cggccttcgg atccttgtca tacaccacgg tggtgtgtcc gtgcttcatc
     5221 agccggcgga cgatattgcc gcccatccgc cccaggccga tcatgccgag ctgcattttg
     5281 aagttcccta gaattcgatt cagatcacgt tgaggtttcg cgtcaatgtt tccgcagtgg
     5341 aggtgggctc cctctcccgt ggggagaggg ttggggtgag ggggttcggt ctaacgatgg
     5401 agcgtaaccc ctcacccggc gctatgcgcc gacctctccc tatgcgagag gtgatcgagc
     5461 tcgggttttc cgatcccgtc gtcatgacca agaccctagt tcaacgcatc ctgaatcgcg
     5521 gtatcgagca gctttagtcc ggtatcgagg tcgcccttga gatgcacgcg caaagcgcgg
     5581 cggccgcgct cggtcagcac atcgaaatcg ccgcgggctt gggcagcctt gatcacgcca
     5641 aaactggcct tttgaccggg caccgagaga tccttgtcgt catcggcggt gatctgcaga
     5701 aacacgccgc tgtcagggcc gcccttgtag gcctgcccgg tggaatgcag gaaacgcggt
     5761 ccgaattcgg cgcaggttgc gacatgacgc ttgtcgcgca cctcaagccg catttgctgc
     5821 aaggcgtcga tatgcgcgga atcgcgttcg atataggcga gcagcgccac gtaatcgtcc
     5881 gcaccggcgc gggcgagatg ggccttcagc caggagccaa tatcgccatc ggcgccggcc
     5941 ttgcgcaagg ccgcggcatt cttatcgtcg gtatagagat cggcctgcgc ggtggatatc
     6001 acgggctgtt ccgcgggcag cgcgccggat ttttcgaacg ccgcggtcaa ttcccgggtc
     6061 ttgatcttgg cgtcctcgac gtcgggctgg ttgaacggat tgatgccgag cacggcgccc
     6121 gcgaccgcgg tcgccagctc aaaccgaaag aattcctgac cgatatgctc gatcgatttc
     6181 atgacaatcc ggaccagggg atggccggcc ttttccagtg ccgccagtct gtcgtcatgc
     6241 gccaggtcgt tctcgccctc ggttctgatg tcgatgaaga agcgatcgtt gccgtaaatc
     6301 gcaacgtcgc ccaagggttc gctgtcgatc gggatcaagc ccttgccatc cttgccggtg
     6361 gattcggcga tcaactgctc ggcccaggcg ccgaagtcgg cgatcttttt tgacgagagt
     6421 agcgtcacct tgtcgcggcc ttcgagaccc gcaaggccca tggcgaggcc aagctgcacg
     6481 cccggatttt cgtgcggcgg cacatccggc ccgcacgagc gcaccatcgc cagcgtatgc
     6541 ttcagcaagg tggcaatgtc gatgccagcc gttgccgccg gcaccagccc gaacggcgac
     6601 agcaccgaat aacgcccgcc gatactgggg ttgccgtgga agatgcgcgc aaaaccttgt
     6661 ttggttgcga tcttctccag cgacgagccg ggatcggtca ccgcgatgaa ccgatgcccg
     6721 gccttgtcgg ggccgatagt ctcggagacg cgggcgaaga aataatcctt catcacattc
     6781 ggctcggtgg tgccgccgga tttgctggag acgatgaaca gcgtattggc gatgttgatc
     6841 gaggcctgaa gcttgcggac ctgcgcggga tcggttgaat caagcacatg cagtttcggg
     6901 aagccgggct gtttttcgaa cgtctgcgcc agcacttccg gtccgaggct cgatccgccc
     6961 atgccaagca cgaccgcgtc agtgaaattt tgtcctttca cacggtgcgc aaagtcctcg
     7021 taatcggcga tatcggctgc tgcagcgctg ttcagccagc ccagccattt gtcttcatcg
     7081 gtcccggtcc agaccgattt atcgtgttgc cagagccggc ggattttgcc ggacgcgcgc
     7141 cagtcctcgg tgctcttttc caacgccttg ccgaggctgc ttccgagcgc aaactcctgg
     7201 cggtcgatac cagccccgag caccgccgcg cgcttgtggg cgaccgcgcc gtaaagcttg
     7261 tcggcggcat cggcaaacag tttgacgccg tccttgacca gatcggatgt gatcgcatcg
     7321 agcgatatgc cggatgtctc gagctccgcg agcacgcccc tcgcatcctc gatattttcc
     7381 tcaagactgt cgcgcggcgt gccatggtcg cggaacgcgt cgagcgtcgc cggtggcacg
     7441 gtgttgacgg tgttggggcc gatcagctcc tcgacataga gcacgtcgct gtaatctttg
     7501 ttcttcgtgc cggtcgaggc ccacagcaag cgctgcggct tggcgccttt ggccgcaagc
     7561 ttgtcccaac gcgtcccgga gaacaggcgc tcgtaatcct gataggcgag cctggcattg
     7621 gcgatcgcga tcttgccttt cagcgcggcc agccgctcct tttcggtagg atcgttggcc
     7681 cgtgcgatct tttcgtcgag cgctttgtcg accgcgctgt cgatgcggct gacgaagaaa
     7741 ctcgcgacgc tggcgacatg ggagggatcg ccgcctttcg cgacatattt ttcgagaccg
     7801 gcaagatagg cctgggcgac ctgcacatag actttttgcg agaacaataa cgtaatgttg
     7861 atgctgatgc cttcgccgat caaatgctgg atcgcgggca ggccctccgg tgtcgccggc
     7921 accttgacca tcaaattctt gcgcttgacg tccttccaaa gccgttcggc ctcggcgatg
     7981 gtggctttgg tatccatcgc aagataaggc gacacctcta aactgacaaa accgtcctcg
     8041 cccttcagtt gatcataaac cggtcgcaac acgtcggcgg catgctggat gtcctcgacg
     8101 gcgagatgct cgaacagttc ggcaatcggg cgatcgccct tcttcagggc cttttcgatg
     8161 gcgccgtcat attcgtccga actgccgatc gccttttcga agatcgacgg gttcgacgtg
     8221 acgcccttga cgccgtcggt atcgatcagc ttcttcaggt cgcccttggc gacgaagctg
     8281 cgggccagga aatccagcca gaccgcctgg ccatggtttt cgagtgcttt gacgggattc
     8341 atgatgccct gttctgttcg agctggccca ttctcggcgt ccggtaacct tcaggcggga
     8401 aggcgaattt tgtcaaccag tccctggggg gatgctgacg ggcagaatat gcgtatcccg
     8461 gcgggacaac gccattcaca acatatcgat ccccgggggc gatatcgatc ccgggcaagc
     8521 ggctgtttgg cggcgatgtc ggcgcgattt tgtgacggac gtcatggtgg cgggcccaag
     8581 ggtcgaccga gtctttagtt ggacgaccaa acgcatcccg aacggataga tctctgatag
     8641 attcacgcac acggaactag tcggctgccg tggagttccg aagtagagcg gctacgagtg
     8701 gcaggcgcct tatgaacttt ggcaggctta gtctaggaaa ttcatcgtgt tgcagtgggg
     8761 ttacatcttc gagaaatcac attatgtatg gttataaccg ccggtttcgc acaactattg
     8821 gccaaaatgc ccgcttatgg ctcattctct gcacacgatg tgcattgctg gtccagcccg
     8881 ccgagtgtcc aattgggcga tgtgctgatg ctgattcgcg acaggatgcg ggggcgctcc
     8941 ggcggcgtgg taccaaccag cccgagccag cagttcagcg gaccatatga ggagaggggt
     9001 ctcatgtaca acga
//
LOCUS       DASWGM010000144         6958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1256674,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000144 DASWGM010000000
VERSION     DASWGM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256674"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..706)
                     /locus_tag="VGA15_04340"
     CDS             complement(155..706)
                     /locus_tag="VGA15_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9586954.1"
                     /translation="MSKFAIRLLTLAMFSMAVVAAPFATRAYAAPDTDAPPPPPATKA
                     KAKKKTSENQPSIEDAAFSRGYRAAYATIYDRNDYAAAIGQLKALGHDDSAAVANLIG
                     YSYRKLGDYKVSQIWYERALKADPDHVKTWQYYGLWQVEQGNRDQAQYHLSRIAALAG
                     TGSEEYRSLAAALEKPPGTGLVY"
     gene            complement(782..1324)
                     /locus_tag="VGA15_04345"
     CDS             complement(782..1324)
                     /locus_tag="VGA15_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9586955.1"
                     /translation="MNKFAIRLLTLAMYAMALIAVPLTTAYAAGSDNPAPPASDTKKE
                     KKKHDKSSSIDDPKFLEGYHKAYATIYDRNDYGSAIEQLKALGQDDRADVANLIGYSY
                     RKLGDYKVSQIWYERALKADPDHVKTWQYYGLWQVEQGNRDQAQYHLNKIAALTGTSS
                     EEYRSLAAALEKPPGTGLVY"
     gene            1627..1959
                     /locus_tag="VGA15_04350"
     CDS             1627..1959
                     /locus_tag="VGA15_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586956.1"
                     /translation="MLKTISAALLAVSVLAAPALAAGPAKTTQAPTVKSEQAKSQQAK
                     SEQAKTKTTAQAKTGVLNANAKMGRHHHKHFAHHRSHKHMGALKTHKFSKVSKASIKH
                     VAPATKRG"
     gene            2201..2602
                     /locus_tag="VGA15_04355"
     CDS             2201..2602
                     /locus_tag="VGA15_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586957.1"
                     /translation="MAILLLPITLAACAGSDEARNIAYTDDRGVANQPYPNNYRAELL
                     AFMKTYLNNPVGVHDAAMAEPVQRTVGGRLRYLSCLRFTPRESDGSYREARERGILYV
                     DGRLDRMVENASEPCAGAVYAPFPELEKLTR"
     gene            complement(2609..3796)
                     /locus_tag="VGA15_04360"
     CDS             complement(2609..3796)
                     /locus_tag="VGA15_04360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9586958.1"
                     /translation="MSADLMDDFPPSRDRLVTLANWRKAPFSAWGFRNVRRLIPTANI
                     AASNHAAGLEMSLQDIGHVGFTSHDGNPTTISRALRATNTDAFVVLRRGRLAMEWYGN
                     GMSATTPHIVFSVSKSICGALGGILWGHGLLDPDDRVIDYIPELASSVYAGCTIRHLL
                     DMAVGIAFNEDYEDPRGDVARYRHAAGWDPPPAGEPPIDLRSFLGQQKPDGRKHGGLF
                     HYVSTNTDVLGWVYERACGQPYADILSEYLWVPLGAERDAYITVDARDAMRAAGGVCV
                     APRDLARFGEMIRRRGIADGRQVVPGAWIDDINEAGDPEAWAASEFAEIFPKGSYRSQ
                     WYRIDRDRGVLVAFGIHGQFIYIHPQAELVIVRMASEATPLDPDRARGWRRGFDAIAE
                     EFM"
     gene            4118..4828
                     /locus_tag="VGA15_04365"
     CDS             4118..4828
                     /locus_tag="VGA15_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEX9586959.1"
                     /translation="MKRFLLGAAAIIMFAVPALAADLPARKYTKAPAYTAPEVIYNWT
                     GFYIGGNLGGAFAGNNSLQGSGGRFMGGVEGGFDYQFAPNWVVGAEAQFDWLSNNNNG
                     RLFPGGVLVTSKNNELGSVTGRVGYTWGPALLYGKGGFAFRDNNNFGVTVAGVPAAFT
                     TTGRRQDGYTVGAGLEYMFAPSWSAKAEYQYYNFGGTTFTTGPAAIVGTRFKDDEHTV
                     KAGINYRFGWGGPVAARY"
     gene            5102..5341
                     /locus_tag="VGA15_04370"
     CDS             5102..5341
                     /locus_tag="VGA15_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586960.1"
                     /translation="MRVFAVGLILCGVAVPCLAQTVLKSEPLVLAPYEIAFVQDASCP
                     AGKVLKVTGAIRGLHRRKACVALAAEQASLAKATP"
     gene            complement(5414..5668)
                     /locus_tag="VGA15_04375"
     CDS             complement(5414..5668)
                     /locus_tag="VGA15_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586961.1"
                     /translation="MPYALFCNDARLSKTYPTEADVWKLARQSGLVVDVVTGEERPGP
                     RPVLDNDYEIRPCRPDPHEDPARNQAEAERDAELDFQLGS"
     gene            6202..6570
                     /locus_tag="VGA15_04380"
     CDS             6202..6570
                     /locus_tag="VGA15_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586962.1"
                     /translation="MIETLKARVNGDEALVRRGRFLTTTFLLEVGQTPWLISIFEGKV
                     VSVTSGPFVMPSSSFSLRAPEQEWEKFWSSRPPPGSNDLMALIKRRVLKAEGNLQIFM
                     ANLRYFKETLAKLRVEGVKA"
     gene            6567..>6958
                     /locus_tag="VGA15_04385"
     CDS             6567..>6958
                     /locus_tag="VGA15_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9586963.1"
                     /translation="MSARFEPIAGRYMHLELSGRQHRIYVEEAGQGTPLLCLHTAGAD
                     GRQYRALMNDARITRGHRVIAFDMPWHGKSSPPSGWHNEEYQLTSAQYTTMILNIMTA
                     LELDRPILIGCSIGGRIALHLALEHPQE"
BASE COUNT         1299 a         2158 c         2146 g         1355 t
ORIGIN      
        1 ttcgcccgag cgtgtaaagc tcgtggtcta tccgggcggc cacatgttct attcgcgcga
       61 cggctcgcgc caggctttcc gggccgaggc cgaggcgctg atgaagtgaa gccgaggcaa
      121 aaaacaaagc cggcgcaacc cagtgtcgtc gaattcagta aaccaggccg gtgccgggcg
      181 gcttttccag tgccgcggcc agcgagcggt attcctcgct gccggtgccg gccagcgcgg
      241 cgatccggct caaatgatat tgcgcctgat cgcggttacc ctgttcgacc tgccacagcc
      301 cgtaatactg ccaggtcttg acgtggtccg ggtcggcttt cagcgcgcgc tcgtaccaga
      361 tctgcgagac cttgtagtcg ccgagcttgc ggtaggagta gccgatcaga ttggcgacgg
      421 cggcgctgtc gtcgtggccg agcgccttga gctgcccgat cgcggcggca taatcattgc
      481 ggtcatagat cgtggcgtag gccgcgcgat aacccctcga gaatgccgca tcctcaatgc
      541 tgggctggtt ttcgctggtc ttcttcttgg ccttggcctt tgttgcgggc ggcggcggcg
      601 gcgcatcggt gtcgggcgcg gcataggctc tggtggcaaa gggggcggca accaccgcca
      661 tcgagaacat tgccagcgtc aaaagcctga tcgcaaattt gctcatgcat ctctcctgtt
      721 ggcggtgtga agatcaggtt tcctgacttc gcgaagtcag gaaacctggt ttttgtgaag
      781 atcagtaaac cagaccggtg cccggtggct tttcgagcgc tgcggccagc gagcgatatt
      841 cctcgctgct ggtgccggtc aaggcagcaa tcttgttcag gtgatactgc gcctggtcgc
      901 gattgccctg ttcaacctgc cacaagccgt aatactgcca ggtcttgacg tggtccgggt
      961 ccgccttcag cgcgcgctca taccagatct gcgacacctt gtagtcgccg agcttgcgat
     1021 aggagtagcc gatcagattg gcgacgtcgg cgcggtcgtc ctggccgagc gccttcaact
     1081 gctcgatggc cgagccatag tcgttgcggt cgtagatcgt ggcataggcc ttgtgataac
     1141 cctcaaggaa cttcggatcg tcgatgctcg aactcttgtc gtgcttcttc ttttccttct
     1201 tggtgtctga cgccggcgga gcaggattat cgctgcccgc agcataggcc gtcgtcagag
     1261 gtactgcgat caacgccatc gcgtacatcg cgagcgtcaa aagcctgatc gcaaacttgt
     1321 tcatgcatct ctcctgattt ccagttgggt aagcttacac cgaagcccgc tgacttgaac
     1381 acccgcgggc tttgaacatt cccgggtaat catcccaatt tgagggaatt tttgtgaacc
     1441 ccgtgcggct gtgcgctgtc cggaacatga ccggaaacat gaccaagatg tcatgcagat
     1501 gaacgaagcc cgtcattgcg cttcagaatg ggttcagcgc gcggcacgta atgtcgccag
     1561 catgatcgat gagcccggcc aacccgaccg gcgacctcgc gatccccgtt gaaagaggag
     1621 acaaccatgt tgaagaccat ttccgccgcg ctgctcgcgg tttccgttct cgccgctccc
     1681 gcgctggccg ctggcccagc caagaccacg caagcgccga ccgtcaagag cgaacaggcc
     1741 aagagccaac aggccaagag cgaacaggca aagacgaaga ccactgcaca ggcgaagacc
     1801 ggcgtgctga acgccaatgc caaaatgggc cgccatcacc acaagcattt cgcccatcac
     1861 cgctcgcaca agcacatggg cgcgctcaag acccacaagt tctcgaaggt ctcgaaggcc
     1921 tcgatcaagc acgttgcgcc tgccaccaag cgcggctgaa actgatccgc gggcgtggcc
     1981 taaccgcatt gccccccgcc gcgaccgtgg atatcaggcc cggcctgccg ccattgcttc
     2041 cccaatgacg gcgggccggc attatttccc ggcaagcctg gaggtcttcg ataaccgaga
     2101 tgcgaattcc ttttcgcagg cagacggtct agaacctccg ggcatcagat ctttgaatga
     2161 cggattgcac ttgttgcact tggggatatc agcaaagcgc gtggcgatcc tgctgctgcc
     2221 gatcacgctc gcggcttgcg cgggcagcga cgaggccaga aacatcgcct ataccgacga
     2281 ccgcggcgtc gccaaccagc cgtatcccaa caactaccgc gctgaattgc tggcgttcat
     2341 gaaaacctat ctcaacaatc ccgttggcgt gcatgatgcc gcgatggccg agccggtgca
     2401 gcgtaccgtg ggcgggcggc tgcgttacct cagttgcctg cgctttactc cgcgcgaatc
     2461 cgacggttct tatcgcgagg cgcgcgaacg cggcattctc tatgtcgacg gccggctcga
     2521 ccggatggtc gagaacgcca gcgagccctg cgccggcgcg gtctatgcac cgttcccgga
     2581 actggagaag ttgacgcggt agggcggcct acatgaattc ttccgcgatc gcatcgaagc
     2641 cgcggcgcca gccgcgcgcg cgatccggat cgagtggcgt tgcttccgaa gccatcctca
     2701 cgatcacgag ttccgcctgc gggtggatgt agatgaattg cccgtggatg ccgaacgcaa
     2761 ccagcacacc gcggtcgcgg tcgatgcgat accattggct gcgatagctg cctttcggaa
     2821 atatctcggc gaactcggaa gccgcccatg cctcgggatc gccggcctcg ttgatatcgt
     2881 cgatccacgc gccaggtacc acctgtcggc catccgcgat gccgcgccgg cggatcatct
     2941 cgccgaagcg ggcgaggtca cgcggcgcga cgcagacgcc gccggcggcg cgcatcgcgt
     3001 cgcgggcgtc gacggtgata taggcgtcgc gctccgcgcc gagcggcacc cacaaatatt
     3061 cgctcaggat gtcggcgtag ggctgaccgc aggcgcgctc atagacccag ccgagcacgt
     3121 cggtattggt cgagacatag tgaaacagtc cgccgtgttt ccgtccgtcc ggcttttgct
     3181 ggccgagaaa gctgcgcaga tcgatcggcg gttcgccggc tggcggcgga tcccagcccg
     3241 cagcatggcg gtagcgcgcc acgtcgcccc tggggtcttc ataatcctcg ttgaaggcga
     3301 tgccgaccgc catatccagg agatgccgga ttgtgcagcc ggcatagacc gacgaggcca
     3361 gttcagggat ataatcgatg acgcggtcat ccggatcgag caagccgtgg ccccagagaa
     3421 ttccgccgag ggcgccgcaa atcgacttgc tcacggaaaa gacaatgtgc ggcgtggtgg
     3481 cactcatgcc gttgccgtac cattccatcg cgagccgccc gcgcctgagc acgacgaacg
     3541 catcggtatt ggtggcgcgc agcgcgcggc tgatcgtggt cgggttgccg tcatggctgg
     3601 tgaaaccgac atgtccgata tcttgcagag acatttcgag ccccgccgca tgattggagg
     3661 ccgcgatgtt ggcggtcgga atcaggcggc ggacgttgcg aaagccccag gcgctgaacg
     3721 gcgccttgcg ccagttcgcc agcgtcacca gcctgtcgcg cgaaggcgga aagtcgtcca
     3781 tcagatcggc ggacattttg tctcactttg tcttgtgcgg ttggcggaac cctcgttccg
     3841 gcccgggaac ccggatcact tttcggcgag gcttgaacct gccccttgtt atggttgacc
     3901 cagaacatag ccataaaaca tagacttaga ccctcggcaa accgtcggcc caacgggcga
     3961 aggcccccga aaagcccgga tggcatggaa cagaaaggcg ttgttgtctc gccgtgacag
     4021 ttgccaggca tcggcggttc ggcattgccc cgaagcaacc aaattgaagc cacgttgttt
     4081 gatgagggtc gatagcgaca atgacgggga tctgctcatg aagagatttt tgctcggcgc
     4141 agccgctatt atcatgttcg cggttcccgc attggctgcc gatttgccgg cgcgtaaata
     4201 taccaaggct ccagcctata ccgcgcccga agtgatctat aattggaccg ggttctatat
     4261 cggcggcaat ctgggcggcg cgttcgccgg caacaacagc ctgcaaggca gcggcggccg
     4321 gttcatgggc ggcgtcgagg gtggcttcga ctatcagttc gccccgaact gggtggtcgg
     4381 tgccgaagcc cagttcgact ggctgtccaa caacaacaat ggccgtcttt tcccgggtgg
     4441 cgttctggtg acctcgaaga ataacgaact tggctcggtc acgggccggg tcggttacac
     4501 gtggggtccg gcgctgctgt atggcaaggg cggcttcgcg ttccgcgaca ataacaattt
     4561 cggcgtcacc gtcgccggcg taccggccgc ctttaccacc actggaagac gccaggacgg
     4621 ctacaccgtc ggcgccggtc tcgaatacat gtttgcgccc agctggtccg ccaaggccga
     4681 ataccagtat tacaacttcg gcggcaccac ctttaccacc ggacctgccg cgatcgtcgg
     4741 tacccggttc aaggacgacg agcacaccgt caaggccggc atcaactacc gcttcggctg
     4801 gggtggccct gtcgcggcgc gctactgaga caggtcgcct cgatacgacc aaaggccggc
     4861 tccgcgccgg ccttttttgt gggcgaaggc cgtctcgggt gaggcgggca ggaccgtcac
     4921 gtggcgcgta tcccacaaag cggataccgg ttttgcggcc cgaggtcgcg caaaccattg
     4981 cggcagtgcg gtttctttcg ccggaccgga ttgcactttg gacccaaaaa taaattttgg
     5041 cgtctgcgaa acttctgctc gcgatcggtt attgtactgc gggcagtggt ggacaacgaa
     5101 catgcgggtt tttgcggtag ggctgatttt atgcggggtt gcggtgccgt gcttggctca
     5161 gaccgtcctg aaatccgagc ccctggtgct tgcgccttat gaaatcgcct tcgtgcagga
     5221 tgcttcatgt ccggccggca aggtgctcaa ggtcaccggc gcgatcagag gcctgcaccg
     5281 caggaaggcc tgcgtggcgc tcgccgccga acaggcgtcg ctggccaaag caacgccgta
     5341 ggatttggcc tcgcggcgtg tcgtcccggc gttccgccgg gacgactcga gaatttagca
     5401 aacggtcttc ccctcacgag cccagctgga aatccagttc ggcgtcgcgc tcggcttcgg
     5461 cctggttcct ggcgggatcc tcgtgcgggt ccggccggca cggcctgatt tcgtaatcgt
     5521 tgtccagcac cgggcgcggc cccggtcttt cttcgcccgt caccacgtcc accaccagcc
     5581 cactctgccg ggcgagtttc cagacatccg cctcggtcgg ataggtcttg ctcagcctgg
     5641 cgtcattgca aaacagcgcg taaggcatcg tatcgctccg cccatccatc ctaacgccca
     5701 aggcgatttt gggttgcaac ggagcgcgcc aatgtgacgg gaagacggcc tgcgaaagct
     5761 atttcggtgc ttcgtcgaac caggcagcac gcttcgccaa attcaagcga tgtcaaggcg
     5821 ggattcgaag aatcgggtaa cgcggccgac tgcgtccgcg tcaactgggc cggccgtcgc
     5881 gggggaagcg gtcccgggcg ggcttatgac gcgagtccct gagtcgtaaa cgagtcctga
     5941 atcaccggaa aaataatccc cgggactctc cggctagaat cgcccgatgt tttgcaggcc
     6001 atgagaatca ttctgcaaaa ctcctgctgg cgcgtgcatc tcacgctcac tattgcgctg
     6061 ctgacggcat gcgcggcgaa cctggtgctg gtatgcgcct ggctttagcc gattgacgcc
     6121 gccgccattc tacccatcat gcggcaaaag cgcgtgaggc gccgggctgc ggcttgcccg
     6181 atattgcgag ggggaggcgg gatgatcgag acgctcaagg cgcgggtcaa cggcgatgag
     6241 gcgctggtca ggcgcgggcg gtttctgacg acgacgttcc ttttggaagt cggccagacc
     6301 ccttggctga tctcgatctt cgaaggcaag gtcgtgtcgg tgacgtctgg gccgtttgtg
     6361 atgccgtcgt cgtcattttc cctgcgcgcg ccggaacagg aatgggaaaa attctggagc
     6421 agccggccgc cgcccggctc taacgatctg atggctctga tcaagcgccg tgttctgaag
     6481 gcggaaggca acctccagat cttcatggcc aaccttcgct acttcaagga gacgctggcc
     6541 aagctgcgcg tcgagggagt caaggcatga gcgcgcgatt cgagccgatc gccggccgct
     6601 acatgcatct tgaattgtcc gggcgccaac atcgcatcta tgtcgaggag gcaggccaag
     6661 gcacgccctt gctctgcctg cacaccgccg gcgccgacgg ccgtcaatac cgcgcgctga
     6721 tgaacgatgc gcgcatcacg cgcggccacc gcgtcatcgc cttcgatatg ccctggcacg
     6781 gcaagtcgtc gccgccttcg ggctggcaca acgaggaata tcagctcacc tcggcgcaat
     6841 acacgacgat gatcctcaac atcatgaccg cgctcgaact cgaccggccg atcctgatcg
     6901 gatgctcgat cggcggccgc atcgcgctgc atctggcgct cgaacatccg caagagtt
//
LOCUS       DASWGM010000145         9934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_844191,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000145 DASWGM010000000
VERSION     DASWGM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844191"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..637)
                     /locus_tag="VGA15_04390"
                     /pseudo
     CDS             complement(166..637)
                     /locus_tag="VGA15_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(886..2013)
                     /locus_tag="VGA15_04395"
     CDS             complement(886..2013)
                     /locus_tag="VGA15_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HEX9586964.1"
                     /translation="MRVTSVGVVRITTKSPSDVSGLLGFIEARKIEPRSILAILGKTE
                     GNGGVNDFTREYAVSALCVALAPYVDLPPHRVEERIAFVMSGGTEGVLSPHITVFTRA
                     AVVGRQSDGISGKRLSIGIAQTRDFLPEELGRGVQIQETAKAVATAMADAAIVDLQDV
                     HYVQIKCPLLTSDRIESASARGAQTVTKSAYGSMAYSRGASALGVAVALREIDGEIGD
                     QHVLRNFELFSSVASTSAGIELMHNVVIVLGNSTSSASPFEIGHAVMRDAIDAASVIN
                     VLKSVGIGDGSGPMGGRQLVNIFAKAEASPNGSVRGFRHTMLEDTDISATRHARAAVG
                     GLIGGLSGTGAVYVSGGAEHQGPPGGGPVAAIARLSTKTSE"
     gene            2471..3505
                     /locus_tag="VGA15_04400"
     CDS             2471..3505
                     /locus_tag="VGA15_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586965.1"
                     /translation="MTTTAALELVQLTKRYGSVTAVDAINLKIPAGSYCCLLGPSGCG
                     KTSTLRMIAGHESATSGDIVVGPKNVTDLPPAERNTAMMFQSYALFPHLSVIDNVAFA
                     LKMKGIAKAERHAEAKKLLQLVDMQAYAARLPGQLSGGQQQRVALARALITSPQILLL
                     DEPLSALDPFLRLRMRAELKKLQRELGISFIHVTHGQDEAMALADIVVLMNGGRIEQQ
                     GSPREIFNRPRTEFTAKFIGGHNVIAVGDETFAVRNDRLMLKKPSEAVNGPSIAGTVS
                     EVEYQGTYVRVAIVADGGADISAQLTESQFDAANYTVGERVLAIWDPAQASPLKTRSS
                     VTVPSPEKAA"
     gene            3517..4815
                     /locus_tag="VGA15_04405"
     CDS             3517..4815
                     /locus_tag="VGA15_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9586966.1"
                     /translation="MTMSSLSKFNGGVSRRAVLKGAAGAVGLAAGSGAITGFPYVQSA
                     EPKVLRYLGTAVNEGDEVSKKCLADTGIKIEYITATTDDVTKRVITQPNSFDVLDTEY
                     FSLKKLIPSGNIFALDAKRIKEFDNITPVFTKGQLPNGKKIGDQGTAPWKVLYLEGPS
                     SKKFSTTPTEFVTLIPTVYNADTLGIRPDLIKRPIESWTELLNPEFKGKASILNIPSI
                     GIMDAAMVVEATGQHKYADKGNMTKAEIDLTMKIMTEAKKAGQFRAFWKDFNESVNLM
                     ASGETVIQSMWSPAVTKVRSMGIACTFQPLKEGYRSWASGFCVSKAVSGPKLDWAYEF
                     VNWYLSGWAGAYLNRQGYYSAVLSTAKANMTADEWGYWMEGKAAVGDIKAPDGTILEK
                     AGAKRDGGSYDDRMGAVACWNAVMDENDYMVRKWNEFIAA"
     gene            4886..5767
                     /locus_tag="VGA15_04410"
     CDS             4886..5767
                     /locus_tag="VGA15_04410"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9586967.1"
                     /translation="MVQDRKSFVAWLQAAPMMLIFALFFLLPLAFVTIVSAWDYNEYE
                     MIPAFSLRGYTDTFEGCIADLPDLCTILKTYLKTLKLCLLTWSLTLLIGFTVAYFLAF
                     HVRSKTWQIVLTLLCTIPFWTSNVIRMIAWIPLLGRNGLVNKGLEGVGLIQRPLEWLL
                     FSEFSVVLALVHLFTFFMVVPIFNSMIRIDKRLIEAAYDAGATGWQTLVNIIIPLAKP
                     GIVIGSIFVITIVMGDFVTIGVMGGQQIASAGKIIESRLNALQFPAAAANAVILLGIT
                     ILIISALTRIVDIRKEL"
     gene            5769..6647
                     /locus_tag="VGA15_04415"
     CDS             5769..6647
                     /locus_tag="VGA15_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9586968.1"
                     /translation="MGEKRSRSFYVLAAFFTAYVLFLYGPMFAIYILSFQGPDGGLTF
                     PMNGVSLQWFSKVFSGTGIVDIGAAFKRSLELGLVVMVLTVVLSVSAGMAFRKSFRGA
                     TFLFYVAIASLIMPSIITSLGIALEFRLLDDFIIKNSAEGWTTAMGLFTSGLGAHLTW
                     TLPFGLLIMFAIFNRFDGRLEEAARDLGATPWQTFRHVVLPIILPSVVGIGLFGFTLS
                     WDELARSSQAIGSVNTLPLELQGLTTTVTKPDIYALGTLTSAVSFLVIFSALVVILVL
                     QARQRRHGSDAGKGMV"
     gene            6658..7392
                     /locus_tag="VGA15_04420"
     CDS             6658..7392
                     /locus_tag="VGA15_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX9586969.1"
                     /translation="MRILIVNPNTTAVMTEKIGRAGRAAAAPGTEIIVVNPPDGPVSV
                     EGYYDAAFSVPGLLAEITKGDALGVSAHVIACFDDTGLEAARSVASAPVIGIGEAAFH
                     LAAMLGHRFSVVTTLSRSIAAIENNLLKYGLASRCGKVRACELPVLSLDDPASDAAAQ
                     IGAEIELAKQEDRAEVIVLGCAGMADLAARLAERHGLPVIDGIASAVKLAEAFGALGL
                     KTSKIGAYAFPRPKAYLGSFASFGPR"
     gene            complement(7618..8169)
                     /locus_tag="VGA15_04425"
     CDS             complement(7618..8169)
                     /locus_tag="VGA15_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEX9586970.1"
                     /translation="MTATAYLAGPDVFLPNAAAHAAGKVEICRRFGLRGLPPLNQDVE
                     TAAKALDVWQAIYEKDIAMMERCDIIIANLTPFGGASADAGTLIEVGWFLGKGRPIFG
                     YSNSSESFEQRMQRQFGKGYPDLGIEGFHLPDNLMIVGAVHSGGCPIFVPSDGKARGI
                     DALDVFEACAAAAAQYVADRKDR"
     gene            complement(8228..9199)
                     /locus_tag="VGA15_04430"
     CDS             complement(8228..9199)
                     /locus_tag="VGA15_04430"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX9586971.1"
                     /translation="MSEPLLRVWNLTKEFPLKGGLFGRHAGSVHAVDGVDFHIDRGET
                     LGLVGESGCGKSTTGRCVLRLIEPTWGEIHFEGKNVRSLQAGQLRILRRDIQIIFQDP
                     YASLNPRMNVGAIVGEALIIHQLTKTRRQFEERVVNLLETVGLQADHMGRYPNEFSGG
                     QRQRIGIARALAVEPKLIICDEPVSALDVSIQAQVINLLEDLQQKFGLAYLFIAHDLS
                     VVEHISKRVAVMYLGRIVETAPARELYTSPLHPYTEALLSAVPIPDPKVKRQRIRLRG
                     EVPSPIQPPSGCHFHTRCPIAQFPLCSSEPPALKQSSDGHWVACHFR"
     gene            complement(9196..>9934)
                     /locus_tag="VGA15_04435"
     CDS             complement(9196..>9934)
                     /locus_tag="VGA15_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586972.1"
                     /translation="MNRIRAREIAIVFQEPMTSLNPVYTIGDQIGEVIALHEGLNRRA
                     VLERTIDMLALVQIPNPKRRVNDYPHQFSGGMRQRVMIAMALSCHPKLLIADEPTTAL
                     DVTIQAQILDLLQDMKSRFGMSIMLITHAMGVVAEVAQRVVVMYAGKVVEQASVEQLF
                     ANPRHPYTQGLIRSIPRIDMAAIRKTRLQTIAGTVPRLIDPPVGCRFAPRCNFATDEC
                     RRAQPALRELEPGHKVACIHAEQTVVQ"
BASE COUNT         2017 a         2966 c         2894 g         2057 t
ORIGIN      
        1 gacgacctaa cattcgcgtg aatgcaattc atcgatcaca aaggtgtgtc gctacatgag
       61 gcacgtgcat atgcggcgac gtatctccct ccctattctc ggccgggcgg tcgcgttgcc
      121 tcttacagcg cgcggacagc agccacagat ctgcatgcaa attccctaga acgccgcctg
      181 agtccgcatc gcgaacgcgt cgagcttcga accggcattg ccggcgtcag tgccgctgat
      241 ctgcttggcg acgtcgccgt gcagatagtc gaacatcagc ggacgttgcg attgacatac
      301 cagttcagcg ccaccgtata attggtctgt cgtccgcgga cgaagccgtc ggcggtggcg
      361 aactgatcat tgagatcgat agtgctgaca cgaccggcaa tttcccacgc gcccagccgc
      421 caccggtcaa tgagaacgga cttgccggca caatgctgcc gcgagcggcg ctgcccgggt
      481 tatagtgacg ggtttcgccg gtcaggacgt agtaggctgt ctccgcatag ccgccctgga
      541 atttcaggct gggcagcggg gtgaaagcgt tgcggtcgac attgaaccag aaatattcgc
      601 cttggaagaa cagcggcccg tacgtccccg ccgcttcggt acgatggcgc gggagatgat
      661 cctcgagcca gtagaaaacg cacagcgcga ggttggcccg gacggccgca ctgaaataag
      721 gggacgtcga tgatcatgag aagcgccaga tagcctacga tccggatggt cgcgatggca
      781 gagagatata acgcatgtgt tgcgtcgacc ggttgagctg gcagcgcaaa gcggccattc
      841 ttcgaacacc aatatcggca aagtgccaca aaatacgttc agtgctcatt cagaagtctt
      901 tgttgatagc cgagcgattg cagctacggg tccgccgccg ggcggaccct gatgctcggc
      961 gccgccggaa acatagacgg cgccagttcc cgataagccg ccgatcaatc cgccaactgc
     1021 ggcgcgggca tgtcgggtag cgctgatgtc cgtgtcctca agcatggtgt gacgaaaccc
     1081 acgcacgctg ccgtttggcg aggcttccgc cttggcgaaa atgttgacca gttggcggcc
     1141 tcccatcggt ccggagccgt cgccgatgcc cacgctcttc aacacattga tcaccgaagc
     1201 ggcatcgatc gcgtcgcgca tgacggcgtg cccgatctca aacggactcg ccgacgatgt
     1261 tgaattgccc aggacgatca cgacgttatg cataagctca ataccggccg atgtcgacgc
     1321 caccgacgaa aacagctcga aatttcgaag gacatgctgg tcgccaatct cgccatcaat
     1381 ctcacgaagc gctaccgcga ccccaagcgc cgaagcacca cgcgaatatg ccatcgaacc
     1441 gtatgcgctt ttcgtaaccg tctgcgcccc gcgggcagat gccgattcga tgcggtcact
     1501 cgtcaacagc ggacacttga tctgcacgta atgtacgtct tgcagatcga cgatggcggc
     1561 atcagccatg gccgtcgcca cggcctttgc tgtctcctgg atttgcacgc cgcgcccgag
     1621 ttcctcgggc aggaaatctc gcgtttgtgc aatcccgatg ctcaggcgtt tcccggaaat
     1681 gccatcggat tggcgcccaa cgacggcggc acgcgtgaag acggtgatat gcggactaag
     1741 cacgccttct gtgccacccg acatcacgaa cgcgatccgc tcttcgacgc ggtgcggagg
     1801 cagatcgacg taaggcgcca gggcaacaca taacgcggaa accgcgtatt cccgcgtgaa
     1861 atcattcacg ccgccatttc cctcggtttt tcccagtatc gcgaggatcg acctcggttc
     1921 aattttgcgc gcctcgataa agcctagcag acccgagaca tcactggggc tcttggtggt
     1981 gatgcgaacg acgcccaccg atgtcacgcg cataccattt tcccctgcaa ccgacatgaa
     2041 gcgttactct gtccacccga cggcgcagcg cattccgccc cccgcgatgc caatcgccgc
     2101 aaaaatatgc cgatgatggg tccaccgcag caggttcagc cgatattctc ggcgtagaga
     2161 aaagatatgg caagcgatga cggctccgcg gcttccgaaa actccttgcg cccgaccgct
     2221 tggatcgccg caaagccgcc agatggtaag ttttcgacat cgcctcaact acttaagcct
     2281 tgaataaaac ggcggcagcg gtgaaaatcg gctcgcgcgt tgcgtcagac ttagggtcgg
     2341 agcaacgcgc cgggcttcat caaacaatca ggattgcctc cctttttggc acatctgttg
     2401 catccctcta cgtaacctgt caggcgcgaa agtcgcctag gtcctcggtt agccatgcgg
     2461 attgatgcga atgacgacca cggcggcgct ggaactggtg caactgacga agaggtacgg
     2521 cagtgtaacc gccgtcgacg ccatcaacct caagattcca gcgggctcat attgctgctt
     2581 gctcgggccc tccggctgcg gcaagacatc cacgttgcga atgatcgccg gccatgaatc
     2641 cgcgacatcc ggagatatcg tcgtcgggcc aaaaaatgtg accgatctgc cccctgccga
     2701 acgcaacacg gcgatgatgt tccaatctta tgcgttgttt ccgcatcttt ccgtgatcga
     2761 caatgtagcc tttgccttga agatgaaggg aattgccaag gcggagcggc atgccgaagc
     2821 caaaaaattg ctccagcttg tcgacatgca ggcttatgcg gcgagactgc ccgggcagct
     2881 ttccggcggc cagcagcaac gcgtggcgct cgcccgcgct ctgatcactt ctccccagat
     2941 tctgctgctg gacgagccgc tttcggcgct tgatccgttt ttgcggctgc gcatgcgggc
     3001 cgaattgaaa aagctgcaac gcgagcttgg gatctccttc atccatgtca cgcacggcca
     3061 ggatgaagcg atggcgctgg ctgatatcgt ggtcctgatg aatggcgggc ggatcgaaca
     3121 gcaggggtct ccgcgcgaaa tcttcaaccg tccccggacg gaattcacgg cgaaattcat
     3181 cggcggtcac aatgttattg ccgtcggcga cgagaccttt gccgttcgca atgatcgtct
     3241 gatgctgaaa aagccgagcg aagccgtcaa tggtccgtcg attgcaggca ccgtcagcga
     3301 agtcgaatac cagggaacct acgtccgcgt cgcgattgtc gccgacggcg gcgccgacat
     3361 ttcggcccaa ctcacggaaa gccagttcga cgccgccaac tacaccgtcg gcgagcgtgt
     3421 tctcgccatc tgggatccgg cgcaggcaag cccactgaag acccgcagtt ctgtaaccgt
     3481 cccgtcccct gaaaaggcgg cttgacacct ttggagatga ccatgagcag tctttcgaaa
     3541 ttcaatggcg gcgtgagccg tcgcgctgtg ctgaagggcg cggccggagc cgtcggcctt
     3601 gccgcaggtt cgggcgcaat taccgggttt ccttatgtcc agtcagccga gccgaaagtt
     3661 ctgcgctatc tcggaaccgc ggtgaacgaa ggcgacgagg tctcgaagaa atgtctggca
     3721 gataccggga tcaagattga atacatcacg gccacgaccg acgatgtgac caagcgtgtc
     3781 atcacccagc cgaactcgtt tgatgtgctc gatacggaat atttctcgct gaaaaaactg
     3841 ataccgtcgg gcaatatttt cgcgctcgat gccaagagaa tcaaggaatt cgacaatatc
     3901 acaccggtct tcaccaaggg gcagttgccg aacggcaaga agatcggcga tcagggcacc
     3961 gcgccgtgga aagtgcttta tctcgagggc ccgagctcca agaagttttc gaccacgcca
     4021 accgaattcg tgacgctgat cccgacggtc tacaatgccg atacgctcgg tatccggccg
     4081 gacctgatca agcggccgat tgaatcgtgg accgaattgc ttaatcccga attcaagggc
     4141 aaagcctcga ttctcaacat cccgtcgatt ggcatcatgg atgcggcgat ggtggtcgaa
     4201 gccaccggcc agcacaaata tgccgacaag ggcaacatga cgaaggccga gattgatctc
     4261 accatgaaga tcatgaccga agccaagaag gccggccagt tccgcgcatt ctggaaggat
     4321 ttcaacgaat cagtcaacct gatggcgtcc ggcgaaaccg tgatccagtc gatgtggtcc
     4381 cccgcggtca ccaaagtgcg ctccatggga atcgcatgca cgttccagcc gctcaaggaa
     4441 ggctatcgct cctgggcttc tggattctgc gtgtcgaagg cggtttcagg accgaaactc
     4501 gactgggcct atgaattcgt gaactggtat ctctcgggct gggcgggcgc ctatctcaat
     4561 cgccagggtt attattccgc cgttctttcg accgccaagg ccaacatgac ggccgacgaa
     4621 tggggctact ggatggaagg caaggccgct gtcggcgata tcaaggcgcc agacggcacc
     4681 atcctcgaga aggccggcgc caagcgtgac ggcgggtcct atgacgaccg catgggcgcc
     4741 gttgcctgct ggaacgccgt catggacgag aacgattaca tggtacgcaa gtggaatgaa
     4801 ttcatcgccg cctgatgcca tctcaggact tgccgtgcca aaccgcgcgg caagtccctc
     4861 tcaggtgacc gagatcggcc tttccatggt tcaagaccgc aaatccttcg tggcgtggct
     4921 tcaggccgcg ccgatgatgc tgatctttgc gctgtttttt ctcctgccgc tggcttttgt
     4981 gacgatcgtc agcgcgtggg actacaatga atatgagatg attcccgcct tcagcctgcg
     5041 cggctacacc gatacgttcg agggatgcat cgccgatctg ccggatctgt gcacgatcct
     5101 gaagacctat ctgaagacgt tgaaattgtg cctgctgaca tggagtctga cacttctgat
     5161 cggctttacg gttgcctatt ttctcgcgtt ccacgtccga tcgaaaacct ggcagatcgt
     5221 gctcaccctg ctttgcacca ttccattctg gacctcgaat gtgatccgca tgatcgcgtg
     5281 gattccgctt ctcggccgga acggtctggt caacaagggc ctcgagggcg tgggattgat
     5341 tcagcggccg ttggagtggc ttctgttctc cgaattttcg gtcgtgctcg ccctcgtgca
     5401 cctgttcaca ttcttcatgg tggtgccgat cttcaattcg atgatccgca tcgacaagag
     5461 gttgatcgag gctgcctatg atgccggagc cacggggtgg cagaccctgg tgaacatcat
     5521 cattcccttg gcaaagccgg gaattgtcat tggctcgatc ttcgtgatca cgatcgtgat
     5581 gggtgatttt gtcacgatag gcgtgatggg cggccaacag atcgcatccg ccggaaagat
     5641 catcgagtcg cgcctcaacg cattgcaatt tccggcggcc gcagccaatg cggttatcct
     5701 gctcggcatc acgatcctga tcatttcggc gctgacgcgg atcgtcgata tccgcaagga
     5761 actttgagat gggcgagaag cggtcgcgat ccttttatgt tctcgcggca tttttcaccg
     5821 cctacgtcct gtttctgtat ggaccgatgt ttgcaatcta cattctctcg tttcaggggc
     5881 ccgatggcgg cctgaccttt ccgatgaatg gtgtttccct gcagtggttc agcaaggtct
     5941 tctccggcac gggcatcgtc gacattggtg cggctttcaa gcggtcgctc gagctcggcc
     6001 tcgtcgtcat ggtgctgacg gtcgttctct cggtttcggc cggcatggcc ttccgcaaat
     6061 cgttccgcgg tgcgaccttc ctgttctatg tcgccatcgc gagcctgatc atgccgtcga
     6121 tcattacctc gctcggcatc gcactggaat tccggttact tgacgacttc atcatcaaaa
     6181 acagcgcaga gggttggacc accgcgatgg gactctttac ttcgggcctt ggcgctcatc
     6241 tcacctggac cttgcccttt ggcctcttga tcatgtttgc gattttcaac cggttcgatg
     6301 gccggcttga agaggcggcg cgcgatctcg gcgcaacgcc gtggcagaca tttcgacatg
     6361 tcgtgctgcc gattatactg ccgtctgttg tcgggatcgg tctgtttggt tttaccttgt
     6421 cgtgggacga gcttgcgcgt tcgagccagg ccatcggttc tgtcaacacg ctgccgcttg
     6481 aattgcaggg attgacgacc accgtgacaa agccggatat ttacgcgctt ggaacgctga
     6541 cgagcgccgt gtcctttctc gtcattttct ccgcgttggt cgtcattctg gtgctccagg
     6601 cccgacaacg gcgtcatggc tcggacgccg gcaagggaat ggtctagggc cgacctgatg
     6661 cgcattctta tcgtcaaccc caacaccacc gccgtcatga ccgagaagat cggcagagcc
     6721 ggacgcgccg cggcggcccc gggaacggag attatcgtcg tcaatccgcc ggatgggccg
     6781 gtctccgtcg aaggctatta tgacgcggcg ttttcagtac cgggacttct ggccgaaatt
     6841 accaaaggcg acgccctcgg ggtttcggcg catgtcattg cctgtttcga tgacaccggc
     6901 ctcgaggcgg cgcgcagcgt cgcgtcggcg cccgtcatcg gtatcggcga ggcggcgttt
     6961 catctagccg cgatgctggg ccatcgcttc tcggtcgtca cgacgctctc gcgttccatc
     7021 gccgccatcg agaacaacct gttgaaatac ggcctggcca gccgctgcgg taaggtgcga
     7081 gcatgcgaac tgccggttct ttcactcgat gatccagcgt cagatgctgc cgcgcagatc
     7141 ggcgcggaaa tcgaactcgc caagcaagag gatcgggccg aagtgatcgt tctcggctgt
     7201 gccggcatgg ctgacctggc cgcacgtctg gcggaaaggc atgggcttcc cgtgatcgac
     7261 gggatcgcga gcgcggtcaa gctggcggaa gcgttcggcg cgctcggcct caaaacatca
     7321 aaaattgggg cctatgcctt cccgcggccg aaagcctatc tcggatcgtt tgcatccttc
     7381 ggaccgcgct agagaccttc gcaacgtcta agcccttcct cgcttcgctg atcgttcgtg
     7441 ccggtcacga tgccgcctcc agattgatgg gagagcaccg gcagtcacga aggcctgaac
     7501 ggcgttgccc ggctgacgta acggccgcaa tctttgtcgc caacgagctt tccgtcacga
     7561 atgatgaaac ggaagcccgc gggggcggag gaggactgca acgcgcgagc tgcgtcgtca
     7621 ccggtccttc cgatcggcaa catattgcgc cgccgcggct gcgcaggcct cgaaaacgtc
     7681 cagcgcgtcg atgccgcgcg ctttgccgtc gctgggaaca aagatcgggc atccgccgct
     7741 atggaccgct ccgacaatca tcagattatc cggcaaatgg aaaccttcga tgccgaggtc
     7801 cggataccct ttgccaaatt gcctttgcat acgctgctcg aagctctcgg aactgttcga
     7861 gtagccaaag attggcctgc cctttccgag aaaccagccg acctcgatca aggtacctgc
     7921 atccgcggat gcgcctccga acggcgtgag gttggcgatg atgatgtcgc atcgctccat
     7981 catcgcaatg tccttctcgt aaatggcctg ccagacatcc aacgccttgg cggccgtctc
     8041 gacatcctga ttgagcggcg ggagcccgcg gagtccgaat cgccggcata tctccacctt
     8101 ccctgcggcg tgcgcggccg cgttggggag aaacacatcg ggccccgcca gataggctgt
     8161 tgctgtcatg acaagtctca ccaaatcgga tcagtccgaa cgaacaaggc tttctgcaga
     8221 cccgcgttca gcggaaatgg caggcgaccc aatgcccatc ggaactttgc ttgagcgccg
     8281 gcggttcact gctgcataac ggaaactgcg cgatggggca ccttgtgtga aaatggcagc
     8341 cggatggtgg ctgaatgggg ctcggaacct cgccgcgcaa tcggatgcgc tgccgcttca
     8401 cttttggatc cggaatcgga accgccgaca acaacgcttc ggtatagggg tggagcggcg
     8461 aggtataaag ctcgcgggcg ggcgcggtct cgacgatgcg accgagatac atcacggcaa
     8521 cgcgcttgct gatatgctcg acgaccgaca gatcatgggc aataaagaga taagcgagac
     8581 cgaatttttg ctgcaggtct tccagcagat tgatgacctg ggcctggatc gacacatcca
     8641 gtgccgatac cggttcatcg cagataatca gtttaggttc gacggcgagc gcgcgcgcga
     8701 taccgatgcg ttggcgttgg ccgccggaga actcgttcgg atagcgtccc atatgatccg
     8761 cctgcagacc gacggtctcg agcaaattca cgacacgctc ttcaaactgc cgcctggttt
     8821 tcgtcaactg gtgaatgatc agggcttcgc caacgatcgc gccgacgttc atccgcggat
     8881 tgagcgatgc ataagggtcc tgaaagataa tctggatgtc gcggcgcagg atgcgaagtt
     8941 ggcctgcctg cagcgatctg acattcttgc cttcaaaatg tatctcgccc caggtcggtt
     9001 cgatcaggcg aagcacgcaa cgcccagtgg tcgatttacc acagccggat tcgccgacca
     9061 gcccgagggt ttcgccgcgg tcgatgtgaa aatcgacacc gtcgaccgca tggacgctgc
     9121 cggcatgacg gccgaacagg ccgcccttga gtggaaattc cttggtcaga ttccagaccc
     9181 tgagcaacgg ctcactcatt gaacgaccgt ctgttccgca tggatgcacg caaccttgtg
     9241 acccggttcg agctcgcgca aggccggttg cgcgcgcctg cactcatcgg ttgcaaagtt
     9301 gcaccgcggt gcgaagcggc agccgaccgg cgggtctatc agcctcggca cggtgccggc
     9361 gatggtctgg agccgggtct tgcggatggc cgccatatcg atgcgcggga tcgagcggat
     9421 caagccttgg gtatagggat ggcggggatt tgcaaacaac tgctcgaccg acgcttgctc
     9481 cacgaccttg cccgcataca tcaccacgac acgctgcgcg acctcggcga cgacacccat
     9541 ggcatgggtg atcagcatga tcgacatgcc gaagcgggat ttcatatcct gcagcagatc
     9601 caggatctgc gcctgaatcg tgacatcaag cgccgtcgtc ggttcgtcgg caatcaggag
     9661 ctttggatgg cacgacaacg ccatcgcaat catcacgcgc tgccgcattc ccccggaaaa
     9721 ctgatgcgga tagtcgttga cgcggcgctt cggattcggg atctgaacca gcgccagcat
     9781 gtcgatggtt cgttccagca cggcgcgacg gttcaatcct tcatgcaggg cgatgacctc
     9841 gccgatctga tcgccgatgg tatagacggg attgagcgag gtcatcggct cctggaacac
     9901 gatggcgatt tccctggcgc gaatgcggtt catc
//
LOCUS       DASWGM010000146         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1950183,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000146 DASWGM010000000
VERSION     DASWGM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950183"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VGA15_04440"
     CDS             <1..1017
                     /locus_tag="VGA15_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX9586973.1"
                     /translation="FNSNGRYGQGGLIRERFAPRLMEADPKTLLDETSDNLDPDKVWA
                     TLMANEKPGGHGERSVAVGTIDMAVWDAVAKIARKPLFRLLAERHGLNPNPRVFVYAA
                     GGYYYPGKGLSMLRGEMRGYLDRGYNVVKMKIGGAPIAEDRMRIEAVLEEIGSQAQLA
                     VDANGRFDLETAIAYARMLRDYPLFWYEEAGDPLDFQLQAALAEFYPGPMATGENLFS
                     HQDARNLIRYGGMRPDRDWLQFDCALSYGLCEYQRTLGVLQTLGWSASRCIPHGGHQM
                     SLNIAAGLGLGGNESYPDLFQPYGGFPDGVRVENGHITMPDLPGIGFEGKSDHYAEMK
                     ALAA"
     gene            complement(1130..>1663)
                     /locus_tag="VGA15_04445"
     CDS             complement(1130..>1663)
                     /locus_tag="VGA15_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HEX9586974.1"
                     /translation="GISFGNFLIKQVVEELRRELPKLENFVTLSPVPGFMKWLKQASD
                     VPVSDEERTLLAHLDQPDWFDNAELTSQLRSLIEPLAAHYFLKARTSKGRLIDSVARF
                     HLGNGARLERIDWLGDRSPKGMRESAGIMVNYLYRLEDIEKNHEAYANQGEIAASSAV
                     KKLLKTEGRRLLDMRLS"
BASE COUNT          316 a          507 c          515 g          325 t
ORIGIN      
        1 ttcaactcca acggccgtta cgggcagggc gggctgatcc gcgagcgttt cgcgccgcgg
       61 ctcatggaag ccgatccgaa gaccctgctc gatgaaacga gcgacaatct cgatccggac
      121 aaggtctggg cgacgctgat ggcgaacgaa aagcccggcg gtcacggcga gcgctcggtc
      181 gcggtcggca ccatcgacat ggcggtgtgg gatgccgtgg ccaagatcgc acgaaaaccg
      241 ctgtttcgat tgctcgccga gcgtcacgga cttaacccaa atccccgcgt gttcgtttac
      301 gcggccggcg gctactatta tcccggcaag ggcctgtcga tgctgcgcgg cgagatgcgc
      361 ggctatctcg atcgcggcta caatgtcgtc aagatgaaga tcggtggcgc gccgatcgcc
      421 gaagaccgca tgcgtatcga ggccgtgctc gaggaaatcg gcagccaggc gcaactcgcc
      481 gtcgatgcca acggccgttt cgatctggag accgcgatcg cgtatgcaag aatgctgcgc
      541 gactatccgc tgttctggta cgaggaggcc ggcgatcccc ttgatttcca gctgcaggcg
      601 gcgctggcgg aattctatcc cgggccgatg gcgaccggcg aaaacctgtt cagccatcag
      661 gacgcgcgca acctcattcg ctacggcggg atgcggcccg accgcgactg gctgcagttc
      721 gattgcgcgc tgtcttatgg actttgcgaa tatcagcgca cgctcggcgt cctgcaaacc
      781 ttgggctggt cggcgagccg ctgtattccg catggcggcc atcagatgtc gctcaatatc
      841 gcagcggggc tcggcctcgg cggcaatgaa agctatcccg acctgttcca gccctatggc
      901 ggttttccgg atggcgtgcg cgtcgagaac ggtcacatca ccatgccgga tttgccgggc
      961 atcggttttg aaggaaagtc cgatcattac gcggagatga aggcgctggc ggcgtgaatt
     1021 agggaggagt cattccgggg cgcgttgaaa acgcgaaccc ggaatctcga gattccgggt
     1081 ctggtccttc ggaccatccc ggaatgacgg caggtggcga ttcaggctct cacgacagcc
     1141 gcatatccaa tagccgccgg ccttcggtct tcagcagttt tttcaccgca ctggaagcgg
     1201 cgatctcgcc ctggttggcg taggcttcgt ggttcttctc gatatcctcg agacgataga
     1261 gatagttgac catgattcct gcggattcgc gcatgccctt tggcgagcga tcgccaagcc
     1321 agtcgatccg ctccagccgc gcgccgttgc cgagatgaaa cctcgccacc gaatcgatca
     1381 gccggccttt tgaggtgcgc gctttcagga aataatgcgc cgccagcggc tcgatcaggg
     1441 agcgcagttg cgacgtcagc tcggcattgt cgaaccagtc cggctggtcg agatgagcga
     1501 gcagcgtccg ctcttcgtcg gacaccggaa catcgctcgc ctgcttcagc catttcatga
     1561 agcccggcac cggcgacagc gtcacgaaat tctccagctt cggcaattcg cgccgcaatt
     1621 cctcgaccac ctgcttgatc aggaagttgc cgaaagaaat tcc
//
LOCUS       DASWGM010000147         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1814732,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000147 DASWGM010000000
VERSION     DASWGM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814732"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VGA15_04450"
     CDS             complement(<1..201)
                     /locus_tag="VGA15_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HEX9586975.1"
                     /translation="MLDSRRNIALACALSALAGYVDGIGFLHLGGLFVSFMSGNSTRM
                     GVSLAEGHWSSASEALGLVALFV"
     gene            complement(458..1246)
                     /locus_tag="VGA15_04455"
     CDS             complement(458..1246)
                     /locus_tag="VGA15_04455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9586976.1"
                     /translation="MNEIDLSLKMRRLAAWLDSRCQPDSLIRKQFHGAPFGSCYVTID
                     PDRQGPFASANLNRVYLCGTEAGMDFDSVERLIELFATEGVKRFFVWLSPGPNMDEVR
                     RWLQGSGLSRIRRTGYPTLCRSERSPVQFRTDLEIREVSVDDVEAARDQLGDTLWPEY
                     ARSAGTKDFFHYMAFDGGRAVAIAALCVFEDLGYLMGAATAESHRKRGAQQALIAKRV
                     ERAEQIGCSIMVSETLHMLEHSYRNLQRAGFQEVYEKEVYEWSA"
     gene            complement(1507..>1872)
                     /locus_tag="VGA15_04460"
     CDS             complement(1507..>1872)
                     /locus_tag="VGA15_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX9586977.1"
                     /translation="SDLAFRTAARIGVSEGLPQCQPVLLEPIHVVEIVCPTDATAKIN
                     AILSGRRGQILGFDTREGWPGWDRVRAMMPEAEIGELIVELRSATAGAGSFTRQFDRM
                     AEVTGRAADQIIAAHRVAA"
BASE COUNT          360 a          594 c          584 g          334 t
ORIGIN      
        1 cacgaacagc gcgaccagcc cgagcgcctc gctcgcgctc gaccaatgcc cctcggccag
       61 actgacgccc atccgtgtcg agttgccgct catgaaggag acgaacaggc cgccgagatg
      121 caggaagccg atgccatcga catagccggc cagcgcgctc agggcacagg ccagggcaat
      181 gttgcggcgt gaatcaagca tcgtcctgcg ctccgtcgcc atgatttaca aatagtaacg
      241 tgctgctttc ttctcccctc cccccttgcg gggtcgagac gagcgaagct cgctcttagg
      301 ggtcgggggt gggggtcaca ggtgcagtga ctcggagtta ccccctcccc aacggaggag
      361 ggagcaggct gtgccggcgt gaatcaagca tggcattgaa ccgccaggcg gtggatgcag
      421 tgccgctgcg tcgatcggcg gaagcaacga ggacgcttca ggcgctccat tcgtagactt
      481 ctttttcgta cacttcctga aagccggcgc gctgcagatt gcggtaggaa tgctcgagca
      541 tatgcagcgt ttcggacacc atgatcgaac agccgatctg ttcggcccgc tcgacccgct
      601 tcgcaatcag ggcctgttgg gcgccgcgct tgcgatgact ttcggcggtc gctgccccca
      661 tcagatatcc aaggtcttca aacacgcaga gggccgcgat ggccacggca cgtccgccgt
      721 cgaacgccat gtaatggaag aagtcctttg tccccgcgga cctcgcatat tcgggccaca
      781 gggtatcgcc cagttgatcg cgggctgctt ccacgtcgtc gacgctcacc tcgcgtatct
      841 cgagatcggt tctgaattga accggcgaac gttcgctgcg gcacagggtc ggatagccgg
      901 tgcgtcgaat acgggagagc ccgctgccct gcagccaacg gcgcacctcg tccatgttgg
      961 ggccggggct gagccagacg aagaaccgct tgaccccttc ggtcgcgaac agctcaatca
     1021 aacgttcgac actgtcgaag tccatcccgg cttccgttcc gcacagatag acgcgattga
     1081 gattcgcgga agcaaacggg ccctggcggt cgggatcgat ggtgacgtaa caactgccga
     1141 agggagcgcc gtggaattgc tttcgaatga ggctgtcagg ctggcatctg ctgtccagcc
     1201 aggcggcgag ccgcctcatt ttcaacgaca aatcgatctc gttcattacg ctcctcgcca
     1261 gctgcgatgc ccgtgcagca gaataccgcg acgcgccccc gcgtcgcagt cgcagtgttc
     1321 atgatggcgg gcggtttggg aagtaacgac cgcgccaaaa cgcgatcacg cgtggggctc
     1381 aactgcggcg ttcgccgacg cgtcgtgcag gattacaatc aaaaaatgac gtatttcggc
     1441 gcaaacttga cgcacaaagc gtcaagtttg tcgcgaattc gcggccggca agggcggcat
     1501 gcaaaatcaa gcggcgacgc gatgggcggc gatgatctga tcggcggcgc ggccggtgac
     1561 ctcggccatg cggtcgaact gccgggtgaa gctgccggcg ccggccgtcg ccgaccgcaa
     1621 ctccacgatc agctcgccga tctcggcttc cggcatcatg gcgcggacgc ggtcccatcc
     1681 cggccagcct tcacgggtgt cgaaaccgag aatctgccct cgccgccccg acaggatcgc
     1741 gttgatcttc gcggtggcat cggtcggaca gacgatctcc accacgtgga taggctccag
     1801 caataccggc tggcattgcg gcagtccctc gctgacgccg atccgcgccg cggtgcggaa
     1861 tgcgagatcg ga
//
LOCUS       DASWGM010000148         5887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_703056,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000148 DASWGM010000000
VERSION     DASWGM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703056"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VGA15_04465"
     CDS             complement(<1..408)
                     /locus_tag="VGA15_04465"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX9586978.1"
                     /translation="MTAAFIFPGQGSQAVGMGKAMAEAFPAARAVFDEVDAALGEKLT
                     AVIWDGPAETLQLTENAQPALMAVSIATLRVLESEAGFSVPAHAAFVAGHSLGEYSAL
                     AAAGSLSVNDAARLLRTRGLAMQKAVPVGVGAMA"
     gene            794..1273
                     /gene="rpsF"
                     /locus_tag="VGA15_04470"
     CDS             794..1273
                     /gene="rpsF"
                     /locus_tag="VGA15_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX9586979.1"
                     /translation="MPLYEHVFLARQDASTQQVEELTTQMTGIVEQLGGKVVKMENWG
                     VRSLTYRMNKNRKAHFVLLNIDGPSTAVAEIERQERISEDVIRYLTVRVEEHEEGPSA
                     MMRKADRDRDRDDRGGGFRGDRDGGFRGDREGGGGGGYRGDRGPRRPRDDADAVVEE"
     gene            1279..1518
                     /gene="rpsR"
                     /locus_tag="VGA15_04475"
     CDS             1279..1518
                     /gene="rpsR"
                     /locus_tag="VGA15_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX9586980.1"
                     /translation="MAEAGARRPFFRRRKTCPFTGPNAPKIDYKDSKLLMRYVSERGK
                     IVPSRITAVSAKKQRELARAIKRSRFLGLLPYVIR"
     gene            1701..2681
                     /locus_tag="VGA15_04480"
     CDS             1701..2681
                     /locus_tag="VGA15_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX9586981.1"
                     /translation="MIAIVLIAIAAGCASALMFASIVSGALISLLLFNLAPLPLMVAA
                     LGWGPLSATIGGIVAATGLGAIFGFPYCIAFVVTVALPAWWLGHLALLGRPVANGASG
                     NGASPVAPDLEWYPVGRILLWISGFAAVTTMATLLTLGVDAATITGTLRRGLLQILQI
                     IGQRDAAPTAESEQLIDALVTIAPAAAAIMAVTTLTLNLWLAAKITATSGRLHRPWPD
                     LKSADLPPTTLAALCVAIALCFTGGLLAILAQIVTATLMMAYALTGFAALHTLTLALK
                     SRAFWLGCTYVIVVMFVWPVLAMVALGLADAIFGIRQRYLQRRPPPLPAS"
     gene            2736..3335
                     /gene="rplI"
                     /locus_tag="VGA15_04485"
     CDS             2736..3335
                     /gene="rplI"
                     /locus_tag="VGA15_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX9586982.1"
                     /translation="MEVILLERVAKLGQMGEVVRVKDGFARNFLLKRGKALRATTDNR
                     AKFDGMKAELEVRNLQAKGEAIKVAEKIDGRNVVVLRQASETGQLFGSVTVRDIIASF
                     EADGVSISRSQVMLDAPIKAIGRHKIAIAVHPEVEVGVTVTVARSADEAERINRGEDI
                     STRQEDQDAAAEALAAAGEFFDPEARRDEEPQEDAASDK"
     gene            complement(3392..3856)
                     /locus_tag="VGA15_04490"
     CDS             complement(3392..3856)
                     /locus_tag="VGA15_04490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9586983.1"
                     /translation="MTADLDSDFAPIAERIRESIAAQGFMSHVGAELCELTRGSCTLA
                     VDRRPELLQQHGLFHGGVTAFLVDNATTIAAATSRGQPALTAEYKLNLLSPATGDRLI
                     CRARVIKPGRQVAVVAADVFCVTGGIEKHTATALASIAMLSGEAPAGIQSPA"
     gene            complement(3853..4461)
                     /locus_tag="VGA15_04495"
     CDS             complement(3853..4461)
                     /locus_tag="VGA15_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9586984.1"
                     /translation="MVARARGDLLAAGLAVFDRDGFEGATVAAIRARARASNGSFFHF
                     FGSKKELAGTLFLEVLQRYHAAVLAAIDTSLSAQEGIGRLIRAHLDWVMNNRREARYL
                     FEISRSEWTEDLRDAQRAQNSRLTEGIERWRAPLIARGELLPMTSTLFFSQIIGPAQI
                     FCRAWLSGRDRSDPRHQTDILIACAIRALVAPEAALKTGGMS"
     gene            complement(4575..5477)
                     /locus_tag="VGA15_04500"
     CDS             complement(4575..5477)
                     /locus_tag="VGA15_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586985.1"
                     /translation="MKAVPAGSLPGYLRQHYTTSSDMASLLSAFLISNGATDTRYVGS
                     QPKQEKDAKSEAKPAAPPEQLDRHGHRLHAAVPAQEAAKPDTQPERTAEPDGEELPPQ
                     AEPGRRGRNAKRLARPVEAPDATKPAAEGQAPVQAASERGPDGRKSGAKQRLSKRGKP
                     GSEDLPKTDAARSDAAKTDAAKTDSAKDEPAKGEPAREDKPQSDTAKYEGDKLPGEGK
                     SEAARIEAPKPTGSSETTALRADPVPPVAPAPQGSSTVPPAVSGGTPELSSTPSGPPP
                     AMTASMPPRAPAAPAGPPTPPISQ"
BASE COUNT         1016 a         1915 c         1826 g         1130 t
ORIGIN      
        1 cgccatcgcg ccgaccccca ccggcaccgc cttctgcatg gcaaggccac gggtgcgcag
       61 caggcgcgcg gcatcgttga cactgaggct gccggcggcg gcgagcgcgg aatattcgcc
      121 gagcgaatgt cccgccacga acgcggcatg cgccgggacc gagaagccgg cttcggactc
      181 cagcacccgc aaggttgcga tcgagaccgc catcaatgcc ggctgggcgt tttcggtgag
      241 ctgcagggtt tcggccggac cgtcccagat gaccgccgtg agcttttctc cgagcgcggc
      301 atcgacctcg tcgaacaccg cccgggccgc cggaaaagcc tccgccatcg ccttacccat
      361 accaaccgcc tgggagccct gccccggaaa aatgaatgct gccgtcatcg gcgcccctga
      421 acgtccctga aatccctaaa gttgtgccga aagacaccgc aggggcctat tgtgtcaagc
      481 cgccaccggt tcccagccac cgggagatgc tgggggtcgg cgctttggct tgcctttccc
      541 tcgcctttcc ctcgcctttc ccttgccttt cccttgccat cgcgcccaaa acccttataa
      601 accgcgccat ccgtggatcc cggccgggag ctgaacggac ggtctcagca atttcacctt
      661 tcgggcgatg ttgatgtggc agggccagtc ggtccgtcgt cccgtgcttc cgccttctga
      721 gcatatcgag tcctttccga agggttcgaa gggcttgccg ccgggaaccg cgccaacaca
      781 ggaaaggaca cccatgcctc tatatgagca tgtttttctc gcgcgtcagg atgcgagcac
      841 ccagcaggtc gaagaattga ccacccagat gaccgggatc gtcgagcagc tcggcggcaa
      901 ggtcgtgaag atggaaaact ggggcgtacg ctccctgacc tatcggatga acaagaatcg
      961 caaggcgcat ttcgtgctgc tcaacatcga cggcccctcg acggcggtcg ccgagatcga
     1021 gcgccaggag cgaatcagcg aagacgtgat ccgttatctc accgttcgcg tcgaggagca
     1081 cgaggaaggt ccctcggcga tgatgcgcaa ggccgatcgc gaccgtgacc gagacgaccg
     1141 aggcggcggt ttccgtggcg accgcgatgg cggattccgc ggcgatcgtg aaggtggcgg
     1201 cggtggcggt taccgcggcg accgtggtcc acgccgtccg cgcgatgacg ccgacgcagt
     1261 ggttgaggag taagaatcat ggccgaagct ggagcacgcc gtccgttttt ccgccgtcgc
     1321 aagacctgcc cgttcacggg tccgaatgcg ccgaagatcg actacaagga ttccaagctg
     1381 ctgatgcgtt atgtgtccga gcgcggcaag atcgtgccga gccgcatcac cgccgtctcc
     1441 gccaagaagc agcgcgaact cgcccgcgcc atcaagcgct cgcgtttcct tggtctgctg
     1501 ccttacgtta ttcgctagca gcatgatccg gaatggattc cggtttttgc gaataagatc
     1561 acgctcaaat aagaactttc tcgatccgcg gcgtaagcgc cgcggatcga aaatatcctc
     1621 gtaaggctcc cgggtcgtcc ggttgccgat ggttggggtt ctcaaaaacc tctaaccgct
     1681 cgaaaggagc gggacagctg atgatcgcga tcgttctcat tgccatagcc gccggctgcg
     1741 catcggcgct gatgttcgcc tcgatcgtct cgggcgcgct gatctcgctg ttgctgttca
     1801 atctggcgcc cctgccactc atggtggcgg cactcggatg gggaccgctc agcgccacca
     1861 tcggcggcat tgtcgcggcc accggcctcg gcgcgatctt cggctttccc tattgcatcg
     1921 cctttgtcgt cacggtcgcg ctgcccgcct ggtggctcgg gcatcttgcc cttctgggac
     1981 ggcctgtggc caatggcgcg tcgggcaacg gcgcgtcgcc ggttgcgcct gatctcgaat
     2041 ggtatccggt cggccgcatc ctgctctgga tatcaggctt tgccgcggtc accacgatgg
     2101 cgaccctgct cacgcttggc gtcgatgccg ccacgatcac cggcacgctg cggcgtggcc
     2161 tgctgcagat cctccagatc atcggccagc gcgacgcggc gccgaccgcc gagtccgaac
     2221 aattgatcga tgctctggtg acgatcgcgc cggcggccgc tgcgatcatg gccgtcacga
     2281 cgctgacgct caacttgtgg ctcgccgcaa aaatcacggc gacttccggg cgattgcacc
     2341 gcccctggcc cgacctgaaa agcgcggatc tgccgccgac cacgcttgca gcattgtgtg
     2401 tcgccatcgc gctgtgtttt accggcggac tgcttgcgat tctggcgcag atcgtcaccg
     2461 caaccctgat gatggcttac gcgctgaccg gatttgcagc cctgcatacg ttgacgctgg
     2521 cgttgaaaag ccgcgcgttc tggctcggct gcacctacgt catcgtggtg atgttcgtct
     2581 ggcccgttct ggcgatggtg gctcttggcc tcgccgacgc cattttcgga attcgccagc
     2641 gctatttgca gcggaggcca ccgccgcttc ccgcgtcttg aactcttggc tttaactcaa
     2701 tcctgtaaat ctcatcacct gaaaggagaa cgaaaatgga agtcattttg ctggagcgcg
     2761 tcgccaagct cggccagatg ggcgaagtcg tccgcgtcaa ggacgggttc gcccgcaatt
     2821 tcctgttgaa gcgcggcaag gcgctgcgcg ccaccacgga caatcgcgcc aagttcgatg
     2881 gcatgaaggc cgagctggag gtccgcaacc tgcaggccaa gggcgaggcc atcaaggtcg
     2941 cggagaaaat cgacggccgc aacgtcgtcg tgctgcgtca ggcatccgaa accggtcagt
     3001 tgttcggatc ggtgaccgtt cgcgacatca tcgcgtcgtt tgaggccgat ggcgtcagca
     3061 tcagccgcag ccaggtcatg ctggacgcgc cgatcaaggc catcggcagg cacaagatcg
     3121 ccatcgccgt gcatcccgaa gtcgaagtcg gcgtcaccgt cacggttgcg cgcagtgccg
     3181 atgaggccga acgcatcaac cgcggcgagg acatctccac ccgtcaggaa gatcaggacg
     3241 ccgccgccga ggcgctcgcc gccgccggcg aattcttcga cccggaggct cgccgcgacg
     3301 aggagccaca ggaagacgca gcttccgaca agtaaggcgc ttctcgatgt cgaattgaac
     3361 ttcccaattg aactgtacaa gcccggcctc gtcaggccgg gctttggatt ccggctggcg
     3421 cttctccgct gagcatcgcg atcgacgcca atgcggtcgc ggtgtgtttc tcgattccgc
     3481 cggtgacgca aaaaacatcc gcggcaacca ccgcgacctg ccgtccgggc ttgatgacgc
     3541 gcgcacggca gatcagacga tccccggtcg caggcgacaa caaattcaat ttgtattctg
     3601 ccgtcagcgc cggctggccg cgcgacgtcg ccgcggcaat tgtcgtcgca ttgtccacca
     3661 ggaaagcggt gacgccgccg tggaacaagc catgctgctg caaaagctct ggccgccgat
     3721 ccacggccag cgtgcatgac ccgcgtgtca gttcgcaaag ctccgcgccg acatggctca
     3781 tgaatccttg ggcggcgatg ctctcgcgga ttctctccgc gatcggcgcg aagtcggaat
     3841 cgaggtccgc ggtcatgaca tgcccccggt tttcaaagca gcctccggtg cgaccagggc
     3901 tcggatcgcg caggcgatca gaatgtcggt ctgatgcctt ggatcggaac ggtcgcgacc
     3961 cgacaaccac gcgcgacaga agatctgcgc cggtccaatg atctggctga agaacaatgt
     4021 cgacgtcatc ggcagcagtt cgccgcgcgc gatcaagggc gcgcgccacc tctcgatgcc
     4081 ttccgtaagg cgtgaattct gcgcccgctg cgcgtcgcgt agatcttcgg tccattcgct
     4141 gcgggaaatc tcaaacagat agcgcgcttc gcgccggttg ttcataaccc aatccagatg
     4201 cgcgcgaatc aaacggccga tgccctcctg cgcgctcaag gaagtgtcga tcgccgcgag
     4261 cactgccgcg tgatagcgct gcagcacttc gaggaacagc gtgcctgcca gttccttttt
     4321 cgatccgaag aagtggaaga agctgccgtt cgaagcccgc gcccgggcgc gaatggccgc
     4381 caccgtcgca ccttcgaagc catcgcgatc aaacaccgcc aatcccgcgg ccagcaaatc
     4441 cccccgcgca cgcgcaacca tgttcgcctc cgggatcgag tggtggatca tcactctaga
     4501 gtaatgctct agagacggtc aagtcgggcc gagcatccgg aattggtctt gctaatactg
     4561 gtctttgcta aagcctattg ggagatcggc ggcgtcggcg gtcccgcagg tgcagccggg
     4621 gcccggggcg gcattgaggc cgtcatcgca ggtggcggcc cggacggcgt cgaagagagc
     4681 tccggcgtcc cgccggaaac cgctgggggc accgttgacg aaccctgcgg cgctggcgcg
     4741 accgggggaa ccggatcggc ccgcagtgcc gtggtctcgc tgctgccggt cggcttcggc
     4801 gcctcgatcc tggcggcctc cgacttgccc tcgcccggga gtttgtcgcc ctcgtatttg
     4861 gcggtgtcgc tctggggctt gtcttctctc gcaggctcgc cctttgcggg ctcatccttg
     4921 gcggaatcgg ttttggccgc gtcagtcttg gcagcgtcgc tcctggcagc atcggtcttg
     4981 ggcaggtctt cgctgcctgg cttgccccgc ttgctcagcc tttgcttggc gcccgatttg
     5041 cggccatcgg gcccgcgctc gctcgcggcc tgaactggag cctgaccctc agcagcaggt
     5101 tttgtggcgt cgggcgcctc caccggccgc gccagccgct tggcattgcg gccgcgccgc
     5161 ccgggctcgg cttgcggcgg tagctcttcg ccatcaggct ccgcggtccg ttcgggctgt
     5221 gtgtcgggct tggctgcctc ttgcgcaggc acagccgcgt gcagcctgtg accgtgccgg
     5281 tcgagttgct cggggggagc agccggcttg gcctccgact tcgcatcctt ttcctgcttg
     5341 ggctggctgc cgacatagcg cgtgtcggtc gccccattcg atatcagaaa cgcgctgagc
     5401 agcgaggcca tgtcgctgct ggtggtgtaa tgctggcgca ggtaacccgg cagagacccc
     5461 gccggaacgg ccttcaaaag accccgcggg ctcttgtggc aggctgagca agtacctgcg
     5521 aaaatctggg aagggctctt gccggcttct agattttgcg cctgcgcttg cgccgggacc
     5581 ggtgctaaac agccgatcag aagaatcacc gtcgctggac tgagcgctcg gctcaacatt
     5641 cctattctcc agatgccgaa atcacttccc agcgcctcaa aatccgaccc tggcggcggc
     5701 ggtcaccttt tagccgatta tgccgcgaat ggaagcgaac atatgacgca acggcgtgaa
     5761 cgcgatattt tggaatatcc gctttgtgta caacgcagta gcgcaccgga actatcccgc
     5821 ttataatttc atacaggcgc ttgggagccg tgcgcgggat ggttgtgtcc cgtgtgcttc
     5881 gagagtg
//
LOCUS       DASWGM010000149         6381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1950442,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000149 DASWGM010000000
VERSION     DASWGM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950442"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGA15_04505"
     CDS             complement(<1..527)
                     /locus_tag="VGA15_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX9586986.1"
                     /translation="MMSFDVFGTLISVRDSSYGAFDRILADAGARHLDVQAFWEYWEH
                     RNIAHYWDPYRSYRDICELSLAETFAHFGIAGDSRLINRYFDAFPDFFLYDDVVRTLD
                     RLSRRYKLAVVSNIDDDLLALTPLKRNFDLVCTAQRAEGYKPDGTLFRYLIKNAGCAK
                     DEILHSGQSQFTDLVG"
     gene            683..844
                     /locus_tag="VGA15_04510"
     CDS             683..844
                     /locus_tag="VGA15_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586987.1"
                     /translation="MSRQTSQPVWQPASYLPSGVLFDLQSRIEATSSRLSPTPVSLRI
                     ANWMMSTGK"
     gene            complement(858..1271)
                     /locus_tag="VGA15_04515"
     CDS             complement(858..1271)
                     /locus_tag="VGA15_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9586988.1"
                     /translation="MMPYAGGCSCGAVHYELAAEPIRGFQCQCRDCQRDTGSGHASVM
                     VFPRAAMRITGPVAEISRTADTGAQKLKGFCGSCGSPLYNKPLSKPDMIGIYVGTLDD
                     PSGFKPQAVIFASRGYAWDHLDPALPKLPNMRPSP"
     gene            complement(1324..1752)
                     /locus_tag="VGA15_04520"
     CDS             complement(1324..1752)
                     /locus_tag="VGA15_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9586989.1"
                     /translation="MLDTKPSLILKRHIKAAPAKVYAAWTDPEKIMRWFGSDQGSTLH
                     AETDLRAGGRFRIVFRMMDGEERDVSGAYREVVPGRKLTFTWNWASPREPESLVTIDL
                     EPDGDGTLLTLTHEQIAGGTVHDYRRGWVGALDKLEKLFA"
     gene            complement(1892..2587)
                     /locus_tag="VGA15_04525"
     CDS             complement(1892..2587)
                     /locus_tag="VGA15_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HEX9586990.1"
                     /translation="MSGRFGIALATLLVSIACGSQARSQDAATLKKSMIGQWELSTTE
                     RSKTCVVTLKSDATPQGLKLELEPGCPAALPFTKDITAWNVKGLDIVRLQDAAGQPVI
                     DFTEVESGILEGMRTNEGIYILQNLAAARSLAKSMDQMIGDWAMVRGNGQAICGLTLT
                     NNEATADNFQVFLKPKCDPAIAAFAPTQWRLDHGQMVLMSAKGETWQFEADDNAQWRR
                     VPDSADPLIMLRQ"
     gene            complement(2587..3576)
                     /locus_tag="VGA15_04530"
     CDS             complement(2587..3576)
                     /locus_tag="VGA15_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX9586991.1"
                     /translation="MVAGHISSEKIDLLIYGPSKPIVDNGFSDQFVLHSFETSHDLDR
                     LTPAVAGKIRGAGVTYNSVRGDGTTLARFPKLEIVSSFGVGYDHIDANYAREHNIIVT
                     NTPDVLTEEVADIAMGLLIATLREFVKADRYLRSGLWLTQQFPLSVGSLRDRKVGMVG
                     MGRIGQAIGRRLDASRVPVVYHSRNPAAGVSYKHYPDLIEMAKAVDTLILILPGGAST
                     AKMINADVMKALGPRGVIINVARGSVVDEPALIAALKSGTILAAGLDVFANEPTVPDE
                     LRAMQNVVLLPHIGSASVVTRNAMDQLVVDNLKAWFSGKPPLTPVAETPVKGR"
     gene            complement(3576..4412)
                     /locus_tag="VGA15_04535"
     CDS             complement(3576..4412)
                     /locus_tag="VGA15_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9586992.1"
                     /translation="MSSLQTPPLSVAQGGPESRALRVVRILLPVVVLAAGIASWELVV
                     RVNDIPPYVLPGPSAVFQTLVSDWPVLLQSLLVTLLTTFEGFAAAAFGGIALALVFNQ
                     SKWLEYSLFPYAVILQVTPVIAIAPLLLIYLSQQTAVIVCAWIVGFFPVLSNTTLGLN
                     SVDRNLAGLFRLYGASRIQTLAYLKLPSALPFILGGLRIAGGLSLIGAVVAEIAAGSA
                     GAGSGLAYRIAESGYRLNIARMFAALLLLSAAGIVIYVLLALTSHLVLRRWHESALGK
                     DN"
     gene            complement(4409..5215)
                     /locus_tag="VGA15_04540"
     CDS             complement(4409..5215)
                     /locus_tag="VGA15_04540"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9586993.1"
                     /translation="MVGPAALSEACSRTEGLAVSLRGVTKVYDSGVTALGPFDLDVNK
                     GEFVSLLGPSGCGKSTALRLIAGLSAPTSGTVGISKPAGQARPGHSIGFMFQEPTLMP
                     WTSVRENVRLPLKLAHAPAAEANARIDQALTQVGLTEFADAYPRELSGGMKMRVSLAR
                     ALVTDPDILLMDEPFAALDEITRFRLNNDLLALWRNLRKTIIFVTHSVFESVYLSQRV
                     IVMTPRPGRLSAEFGIDTPEPRGEDFRTSAEYAGYCRKVSGALAPEKPGS"
     gene            complement(5233..6255)
                     /locus_tag="VGA15_04545"
     CDS             complement(5233..6255)
                     /locus_tag="VGA15_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9586994.1"
                     /translation="MNPVYLLRALTAGLVAALVAISPAGAQTALDKVSFGTNWVAEAE
                     HGGFFQALADGTYKKYGLDVTIVPGGPNDNNRMLLIAGKLDFFMAANTLMSFDAAANN
                     VPVVTVAAIFQKDPQVFLTHPESKVAKLEELKPLTLFVSKEGISSYFQWLKSEYGFSE
                     EKVKPYTFNSQPFLADRLSAMQGYVTSEPFAIEKAAKFKPGIILLADNGFNTYSTLIE
                     TRRELIDKKPDLVQRFVDASIVGWYNYLYGDNAPANAMIKKLNPEMTDDLLAYSVAKM
                     KEYGIVDSGDALRDGIGAMTDARVASFFDKMVRAGVVRRDIDFRKSYTLRFANKGVGL
                     DLRPKN"
BASE COUNT         1297 a         1947 c         1924 g         1213 t
ORIGIN      
        1 ccgacgagat ctgtgaattg cgattgcccg gagtgcagga tctcatcctt cgcacaaccc
       61 gcattcttga tcagatagcg aaacagcgtt ccatcgggct tgtagccctc ggctcgctgt
      121 gccgtgcata ccaggtcgaa gtttcgcttc aatggcgtta gcgcaaggag atcgtcgtca
      181 atattcgaaa ccaccgccag tttgtatcga cgagacaacc gatcaagcgt gcggacgaca
      241 tcgtcataga ggaagaagtc cggaaaggca tcaaagtagc gattgatcag tcggctgtcg
      301 cccgcgattc cgaaatgggc gaaggtttcg gcaagcgaga gttcgcagat atctcgatac
      361 gaccggtacg ggtcccaata atgcgcgatg ttccgatgct cccaatattc ccagaacgcc
      421 tggacgtcga gatggcgcgc gccggcatct gcaaggatgc ggtcaaaagc gccgtaggag
      481 ctgtcgcgaa cgctgatcag cgtgccgaag acgtcgaatg acatcattat cggccgcaga
      541 cgtgcgcgct gcgttgcatt gctcatcgag cgaacccccg acgattgcta ttccgtaagc
      601 gctacaggag ttacattcat ggtatctccg ttcgtaattg cgaacgtcaa ctggtgggct
      661 acccttgttc aggggataaa tcatgagtcg gcagacaagc caaccggttt ggcagcccgc
      721 ttcgtatctt ccctccggcg ttctcttcga tctgcaatct cgcatcgagg cgacctccag
      781 ccggctttca ccgacgccgg tgtcgctccg gatcgccaat tggatgatgt cgaccggcaa
      841 gtgaagtgcg gcgacggcta tggcgagggc cgcatgttcg ggagcttggg cagcgccgga
      901 tccaggtgat cccaagcata cccgcgcgat gcgaagatca cagcctgcgg cttgaagccg
      961 gatggatcat cgagagtgcc aacgtagatg ccgatcatgt ccggcttgga caggggtttg
     1021 ttgtagagcg gcgagccgca agacccgcag aagcctttca gcttttgcgc gccggtatcc
     1081 gcggttcgtg aaatttcggc gacagggccg gtgatacgca tggccgcgcg cgggaacacc
     1141 atgaccgacg cgtgcccgga acccgtatcc ctttggcagt cgcggcattg acactggaag
     1201 cctcgaatgg gctcggcggc gagctcataa tggactgcgc cacaggaaca gccgcccgcg
     1261 tacggcatca tgcgaacctc ctgatttcca tctcgagctg cattgcgttc ccctcctctg
     1321 aaatcaggcg aacagctttt cgagcttgtc gagcgctccg acccagcccc ggcgatagtc
     1381 atgcacggtg ccgcctgcga tttgctcgtg agtcaaagtc aacaacgtcc cgtcgccgtc
     1441 cggctcgaga tcgatcgtca ccagcgattc gggctcgcgg gggctggccc agttccaggt
     1501 aaaggtcagc ttccggccag gcaccacctc gcgataggcg ccgctgacgt cgcgctcttc
     1561 gccgtccatc attcgaaaga cgatgcgaaa gcgcccgcct gcccgcaagt ccgtctcggc
     1621 atgcaacgtt gacccctgat cggacccaaa ccagcgcatt atcttttccg ggtccgtcca
     1681 tgcggcgtag actttcgccg gcgccgcctt gatgtggcgc ttgaggatga ggctgggctt
     1741 ggtgtcgagc atggcggtca agcgctttca caatacttaa ccatatggta aagtatatag
     1801 ctacggtcgc accagtcaag cgcgatctcg aaaaggcgat ggcggacgac aatacccaac
     1861 gccgccccgt gccgaagcta tcgtctgaaa ttcactgccg cagcatgatc agcggatcgg
     1921 cgctatcggg cacccgccgc cattgcgcat tgtcgtcggc ctcgaactgc caggtttcgc
     1981 ctttggccga catcagcacc atctggccgt ggtcaagccg ccattgcgtc ggcgcgaacg
     2041 cggcgatcgc cggatcgcat ttcggcttga gaaatacctg aaaattatcc gccgtcgctt
     2101 cgttgttggt gagcgtcagg ccgcagatcg cctggccgtt gccacgcacc atcgcccagt
     2161 cgccgatcat ctgatccatc gacttggcaa gcgagcgcgc ggccgccagg ttctgcagga
     2221 tatagatgcc ctcgttggtc cgcatgccct ccagaatccc gctctcgact tcggtgaagt
     2281 cgatcaccgg ctgtcccgcg gcatcctgca gccgcacgat atccagcccc ttgacgttcc
     2341 aggcggtgat atccttggtg aagggcagcg ccgcggggca gccgggctcc agttcaagct
     2401 tgaggccttg cggcgtggcg tcgctcttca gcgtgacgac gcaggtcttg ctgcgttcgg
     2461 tggtcgagag ctcccactgg ccgatcatgc ttttcttcaa ggtcgcggca tcctgcgacc
     2521 gggcctgcga accgcaagcg atcgatacca gcagcgtcgc gagcgcaatg ccaaaacgac
     2581 ccgacatcag cggcccttca ccggggtctc ggcaaccggc gtcagcggcg gttttcccga
     2641 aaaccatgcc ttcagattgt cgaccacaag ctgatccatg gcatttcggg tcaccaccga
     2701 ggccgagccg atgtgcggca gcagcaccac gttctgcatg gcgcgcaatt catccggcac
     2761 ggtcggttcg ttggcgaaca catcgagccc cgcggcgagg atggtccccg acttcaacgc
     2821 cgcgatcagg gccggctcgt cgaccaccga gccgcgcgcc acgttgatga tcacgccgcg
     2881 cggcccgagc gccttcatga catcggcgtt gatcatcttc gccgtcgaag cgccgccggg
     2941 aagaatcaag atcagcgtat cgaccgcctt cgccatctcg atcagatcag gataatgctt
     3001 gtacgacact ccggccgccg ggttgcgcga atgatagacc accggaacgc gggacgcatc
     3061 gagccggcgc ccgatcgcct ggccgatccg gcccatgccg accatcccga cctttcgatc
     3121 cctgagcgag ccgacgctca agggaaattg ctgcgtcaac cagaggccgg aacgcagata
     3181 gcggtcggcc ttgacgaact cgcgcaaggt cgcgatcaaa agccccatgg cgatatccgc
     3241 gacctcttcg gtcagcacgt caggggtgtt ggtgacgatg atattgtgct cacgggcgta
     3301 attggcatcg atgtgatcgt agccgacgcc gaaagaggag acgatctcga gcttgggaaa
     3361 ccgcgccagc gtcgtgccgt cgccccgtac cgaattgtag gtcaccccgg cgccgcggat
     3421 tttgccggcg acggccgggg tcagtcgatc gaggtcgtgg ctggtttcaa acgaatgcag
     3481 gacgaactgg tcggaaaaac cgttgtcgac gattggcttg ctcggcccat agatcagcag
     3541 atcgatcttt tcggaagaaa tatgaccggc aaccatcagt tgtcctttcc aagggcgctc
     3601 tcgtgccagc gccgcaacac caagtgcgag gttagcgcca gcagcacgta aatgacaatc
     3661 ccggcggccg acagcaacag caacgcggcg aacattctgg caatgttgag gcggtagccg
     3721 gattccgcga ttcgataagc caatcccgag cctgcgccgg ccgaccccgc cgcgatctcg
     3781 gcgaccacgg cgccgatcag cgaaagtccg cccgctatcc gcaatccgcc aaggataaat
     3841 ggcaacgccg aaggcagttt gagataggcc agcgtctgta tccgcgaggc gccgtaaagc
     3901 cgaaacaggc cggccagatt gcgatccacc gaattgaggc caagcgtggt gttggacagc
     3961 accggaaaaa atccgacgat ccaggcgcag acgatgacgg cggtctgctg cgaaagatag
     4021 atcaagagca gcggcgcgat cgcgatgacc ggcgtgacct gcaggatcac cgcataggga
     4081 aacagcgaat attccagcca tttcgactgg ttgaacacca gcgccaaagc gatgccgccg
     4141 aaggccgcgg cggcgaatcc ttcgaatgtg gtcaatagcg tgaccagcag cgattgcaac
     4201 aacaccggcc agtcgctgac gagggtctgg aacaccgccg atggccccgg cagcacatag
     4261 ggcggaatat cgttgacgcg caccacgagc tcccaactcg cgatgccggc cgcaagcacg
     4321 acgaccggaa gcaggatccg gacaacacgc aacgcgcggc tctccggccc gccttgcgcg
     4381 acggataatg gcggtgtttg caacgagctc atgaaccggg cttttcgggc gccagtgcgc
     4441 cggaaacctt gcggcaatag ccggcatatt cggccgaggt gcgaaaatcc tctccccgcg
     4501 gctcaggcgt gtcgatgcca aactcggcgc tgaggcgtcc gggccgcggt gtcatcacga
     4561 tcacgcgctg cgacaaatac accgactcga ataccgaatg cgtgacgaag atgatggttt
     4621 tgcgcagatt gcgccacagc gcgagcagat cattgttgag ccggaaacgg gtgatctcgt
     4681 cgagcgcggc gaacggttca tccatcagga ggatgtcggg atcggtgacc aatgcgcgcg
     4741 ccagcgagac ccgcatcttc atgccgccgg ataattcgcg tgggtaggcg tcggcaaatt
     4801 cggtcaaccc cacctgcgtc agcgcctgat cgatgcgcgc attcgcctcg gcggccggcg
     4861 catgcgcgag tttcagcggt aaccgcacgt tttcgcgcac gctggtccac ggcatcagcg
     4921 tcggctcctg aaacataaaa ccgatggaat gtcccgggcg cgcctgaccg gcgggtttgg
     4981 aaatgccgac ggtgccggag gtcggtgcgc tgagccccgc gatcagcctc agggcggtcg
     5041 actttccgca gcccgacggt ccgagcaggg aaacgaactc gcccttgttc acatcaaggt
     5101 caaatggtcc cagtgccgtc acgccgctgt catagacctt ggtgacgccg cgcaggctta
     5161 ccgcgaggcc ctcggtacgg gaacaggctt cggacaacgc tgcgggcccg accatcacga
     5221 ctctagttct ttctagttct tgggccgcag atccaggccg acccccttat tggcaaagcg
     5281 aagcgtgtac gacttgcgga agtcgatgtc gcggcggacc acgccggccc gcaccatctt
     5341 gtcgaagaag cttgcgaccc tcgcatcggt catggcgccg ataccgtcgc gcagcgcgtc
     5401 gccggaatcg acaattccgt attccttcat cttggcgacg gagtaggcca gcaagtcgtc
     5461 ggtcatttcc ggattgagct tcttgatcat cgcgttggcg ggcgcgttat cgccgtaaag
     5521 ataattgtac caaccgacga tcgaggcgtc gacaaagcgc tgtaccaggt ccggcttctt
     5581 gtcgatgagc tcacggcggg tttcgatcag ggtagagtag gtgttgaagc cattgtctgc
     5641 cagcaaaatg atccccggct tgaatttggc cgctttctcg atcgcgaacg gctccgaggt
     5701 gacatagccc tgcatcgcgc tgaggcggtc ggcgagaaac ggctgtgagt tgaacgtata
     5761 gggcttgacc ttttcctcgc tgaatccgta ttcggacttc agccattgaa aatagcttga
     5821 gatcccttcc ttggacacga acagcgtcag cggcttgagc tcttccagtt tggcgacctt
     5881 cgattccgga tgcgtcagga aaacctgcgg atccttctgg aagatggcgg cgacggtcac
     5941 caccggcacg ttgttggcgg ccgcgtcgaa tgacatcaag gtgttcgcgg ccatgaagaa
     6001 gtcgagcttg ccggcgatca gcagcatgcg gttgttgtcg ttgggaccgc ctggcacgat
     6061 ggtgacgtcg agcccatatt tcttgtaggt gccgtcggcc agggcctgga aaaatccgcc
     6121 gtgctcggcc tcggcgaccc agttggttcc gaacgaaacc ttgtccagcg cggtttgggc
     6181 acccgccggc gagatggcga caagagcggc cacgaggccg gcggttaacg ctcgcagcaa
     6241 atagaccggg ttcatgattg gactccgtcg atcgttttgg cccatgatag gaagctggac
     6301 gatgttggta cgggttcgcc aggaatgaat gcaggctacc ctgcaaattg acttaaagaa
     6361 atgtttcgct cgatgacttc a
//
LOCUS       DASWGM010000150        11037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_427304,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000150 DASWGM010000000
VERSION     DASWGM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11037
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427304"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VGA15_04550"
     CDS             <1..432
                     /locus_tag="VGA15_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9586995.1"
                     /translation="NPNDPYRSPDDPYRSNLTDDDIRRQARLDSELQADPELAEGPAN
                     GGRIAMFAIAIALVLGAVFYGLNSSTVNQAGTSSSTAQNTSQSSPPAAPPGMRDVTPR
                     TTDQGVTTGAAPARPQIPPASAPTEMNRSGNPPANNAAPSK"
     gene            complement(518..1333)
                     /locus_tag="VGA15_04555"
     CDS             complement(518..1333)
                     /locus_tag="VGA15_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX9586996.1"
                     /translation="MPVTTAEKRATFRKMHETGCFAIPNPYDVGSALALQHLGFKALA
                     STSAGFAWTIGKADNRVTLDEVCTHLTALCAAVDLPVNADFEGGFAHEAQKVGVNVAR
                     AVKTGVAGLSIEDSTGDAAEPLYERAHAIERIKAARQAIDADNSGVLLTGRCEAFLVG
                     QADLDLVIDRLKTYSDAGADCLYAPGIKTREQISAVVKAVHPKPVNLLIGASGLSIAE
                     AGDLGVRRISVGGSLARTAWAGFMGAAREIAEKGTFTELGKGYPGAELNKMFK"
     gene            complement(1400..1750)
                     /locus_tag="VGA15_04560"
     CDS             complement(1400..1750)
                     /locus_tag="VGA15_04560"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX9586997.1"
                     /translation="MIAVIFEVWPKPEYRGEYLDLAAELRPILETIDGFISIERFESL
                     TEKGKILSLSFFRDEEAVAAWRNIPQHRKTQAKGRARIFDNYRLRIAGIIRDYSMTDR
                     AQAPKDSRATHDAH"
     gene            complement(1795..2103)
                     /locus_tag="VGA15_04565"
     CDS             complement(1795..2103)
                     /locus_tag="VGA15_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX9586998.1"
                     /translation="MAITVFIRYQLDPFKRAMFEEYAKRWLTIIPKCGGDLVGYWMPH
                     EGTNNIAFALISFESLAAYETYRARLRADQEGMANFNFAEDNKFILAEERTFLRKVTL
                     "
     gene            2178..2873
                     /locus_tag="VGA15_04570"
     CDS             2178..2873
                     /locus_tag="VGA15_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9586999.1"
                     /translation="MKAGPDIAMVAALVGDPARSNMLTALMTGRALTASELAHQAGVT
                     PQTASSHLSKLEAGGLIEPEKQGRHRYYRLTGPDVAGVLEGLAGLAARAGHMRVRTGP
                     KDPALRRARICYDHLAGDLGVQMLDSLIRQRLVRQSKQAIELTAEGKRFMEKSLQISA
                     EALAHPRRPVCKACLDWSERRHHLAGTLGAAMMTRFAELKWAARDSTPGSRVVNFTRN
                     GEKRFAELFGNDE"
     gene            2956..3960
                     /locus_tag="VGA15_04575"
     CDS             2956..3960
                     /locus_tag="VGA15_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587000.1"
                     /translation="MRRFFFVISAAVLALPFSANAAEPAAREPYGIGLEGFAYPYPVN
                     MLPLSNDGEQVRMAYMDVAPGQPNGRTVVLLHGRNFPSSYWAPVIKTLTDAGYRVVVP
                     DQIGFGKSSKPSADLHFDTLARNTIALLDHLQIAKAEFVAHSLGGMLGVRIARAYPDR
                     VAHLLLAAPIGLEDYRLYVPPTPTEKILENEDRLTADGYRKQLQTNYSLKLPPDQVTP
                     FIDARFNIKGSSEYPRWLRAFVSSAQMIYREPVVHEIPLITRPTLFIMGADDHNAPGK
                     PNAPEALRPKMGQNAELAKALAGQMPDAKAEVLPNAGHLVFLDAPAKFNELMLAFLAK
                     "
     gene            complement(4022..4261)
                     /locus_tag="VGA15_04580"
     CDS             complement(4022..4261)
                     /locus_tag="VGA15_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587001.1"
                     /translation="MKLLTASAAAVGLSLMAMSASQAVPLAPLDQSVSSDTIRVAGGC
                     GPGWHRGPYGGCRPMYNCPPGWHSGPFGRHCFRNW"
     gene            complement(4667..4984)
                     /locus_tag="VGA15_04585"
     CDS             complement(4667..4984)
                     /locus_tag="VGA15_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587002.1"
                     /translation="MPAAYFVVRATVSDPAKRAAFDRWYGKEHLPDAAKSFGVEKAWR
                     FWSLSDPALHQATYQFSDKAALDRAMNGSDLKRLVADFDRDWPGITRTRELLVLAEEF
                     SAG"
     gene            complement(5062..5508)
                     /locus_tag="VGA15_04590"
     CDS             complement(5062..5508)
                     /locus_tag="VGA15_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEX9587003.1"
                     /translation="MSTLFAFLHHLCAFTLVSAVAIEFTLVHQELTLSSARRLQVTDT
                     VLGIAAGALFIVGSLRVFYFEKGADYYFHSHAFTAKFSIFIAIGLLSIIPTVEFLSWR
                     SALGAGQVPVISAKKLRLITGVIHGELLSIVIILLCAAIMARGGWV"
     gene            complement(5834..7366)
                     /locus_tag="VGA15_04595"
     CDS             complement(5834..7366)
                     /locus_tag="VGA15_04595"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9587004.1"
                     /translation="MKEILDTLEERRAGARLGGGEKRIEAQHTRGKLTARERIELLLD
                     KGSFEEFDMFVEHRSVEFGMEKTRVPGDGVVTGWGTVNGRKTFVFAKDFTVFGGSLSE
                     THAQKIVKIQDMAMKARAPIIGLYDAGGARIQEGVAALAGYSYVFRRNVIASGVIPQI
                     SVIMGPCAGGDVYSPAMTDFIFMVKNTSYMFVTGPDVVKTVTNEVVTAEELGGASVHA
                     TRSSIADGAFENDVETLLQMRRLIDFLPSNNTDGVPEWPSFDDIGRVDMSLDTLIPDN
                     PNKPYDMKELILKVVDEGDFFEISETFAKNIITGFGRIAGRTVGFVANQPMVLAGVLD
                     SDASRKAARFVRFCDAFNIPIVTFVDVPGFLPGTAQEYGGLIKHGAKLLFSYSQCTVP
                     LVTVITRKAYGGAFDVMASKEIGADMNYAWPTAQIAVMGAKGAVEIIFRSDIGDTEAI
                     AGRTKEYEDRFLSPFVAAERGYIDDVIMPHSTRRRIARALAMLRDKHVEMPMKKHDNL
                     PL"
     gene            7495..7908
                     /locus_tag="VGA15_04600"
     CDS             7495..7908
                     /locus_tag="VGA15_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HEX9587005.1"
                     /translation="MTDIPGAETTGAVTGGLRTLLRLEGLTLFTGMTLLYAVWGGSWW
                     IYAILFLVPDLSFAAYLWGPRLGAIIYNAAHSYMAPMTLMTGGFALASPLVLSIAMIW
                     LAHIGIDRALGYGLKYNAGFGFTHLGRIGKDAVKT"
     gene            complement(8032..9273)
                     /locus_tag="VGA15_04605"
     CDS             complement(8032..9273)
                     /locus_tag="VGA15_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9587006.1"
                     /translation="MAKKAKKAKTAKRTAKKTARSGGRTPRATPLRFAHPFFTTTPVP
                     DRAVSARTSTTSLAQFASDKLGIVPRPTRDPLMELKDIIGQPGTDEIQQAGAIKFHVI
                     GDTGRQGGDNSPQEQVAQAMTGDYDPAAGGHNPALLIHLGDVIYGHDKSKQYRDEFYR
                     PYMKYPGKIIAIPGNHDGETFKGTDPVSLQEFRNNFCAPSALVPSIAGDVRIFRETMT
                     QPGVYWMLSAPFANIIGLYSNIAEGPGNLLGANGDDQQIKWLTKTLTSLKQSGDKKAL
                     VIATHHPPYSSGGHSPSKDMLAQIDKACNDAGVVPHAMLAGHSHNYQRYTRTAALAGA
                     QVQIPYLVAGCSGHADSAVTAATGQQMGQTIFEKSLKGFGYLTMTANAAQLAIEMVET
                     TNGVKTPFDKVTVDIASHRVT"
     gene            complement(9409..10122)
                     /locus_tag="VGA15_04610"
     CDS             complement(9409..10122)
                     /locus_tag="VGA15_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX9587007.1"
                     /translation="MPDQQPNRYIPAHVEAFPYRLPNFANRLNGSGSIKIVAIGSSST
                     IGEGDIVPFTYRLETALKDKYPNRMIDVLNRGVKGQEAPEELERLHDHVIAEAPALAI
                     WQVGTNAVWQPGHNLDDVAAAIEKGLKLLGGEPMDVVMMDLQYAPAVLTDDKIDATRR
                     MLALINQLATAVNVNVFHRFDLMRKFLDVERHSFDTIIDPTDITRLHQSDFSARRIGY
                     ELSEAIAAGAAHGGQAPAG"
     gene            complement(10530..>11037)
                     /locus_tag="VGA15_04615"
     CDS             complement(10530..>11037)
                     /locus_tag="VGA15_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9587008.1"
                     /translation="LGEQVMKVSSVGLAGPYNPMLRSPVLGQRLFDLFHYLRWETSVP
                     TKLNEFAILIIGRQWRSQVEWYAHAPLAAKAGLSPDIIAELKASKRPSGMADDEALVY
                     DFVTELTTTQKVSDETYARAKKVFSDQQIVDLTAVAGNYVMVAMMLAMAEETTPPGKE
                     PPFKVGEK"
BASE COUNT         2080 a         3394 c         3329 g         2234 t
ORIGIN      
        1 aatccgaacg atccctatcg ttccccggac gatccctatc gttccaatct taccgacgac
       61 gacatccgtc gccaggcccg cctcgacagc gagttgcagg ctgatccaga acttgccgag
      121 ggtcccgcca atggcggcag aattgcgatg ttcgcgatcg ccatcgctct ggtgctgggt
      181 gctgtttttt acggcctgaa tagttcaacg gtcaatcagg ccggcacttc ctcttcgacc
      241 gcccagaata cctcgcagag ctcgcctccc gcagcgcctc cgggcatgcg tgacgtgacg
      301 cctcgcacca ccgatcaagg cgtgacgacg ggtgccgctc cggcgcgtcc gcagataccg
      361 ccggcttcgg ctccgacgga gatgaaccgt tccggcaatc cgccggccaa taacgcagcg
      421 cccagcaagt aacgccggac ccgcataaaa gacccgcata agatacgacc ggaagcccag
      481 caggcattga tggcctgctg cgctttcgtt gtggccgtta cttgaacatc ttgttcaatt
      541 cggcgcctgg atatcccttg cctagttcag tgaatgttcc tttctccgcg atctcgcgcg
      601 ccgcccccat gaaaccggcc cacgcggtcc gcgccagcga accgccgacg ctgatgcggc
      661 ggacgccgag atcgccggct tcggcaatcg agaggccgga cgcaccgatc agaagattga
      721 cgggttttgg gtgcacggct ttcacgaccg ccgaaatctg ttccctggtc ttgatgcccg
      781 gcgcatagag gcagtccgcg cccgcgtcag aataggtttt gagccggtcg atcaccaggt
      841 cgagatcggc ctgcccgacc agaaacgcct cgcaacggcc ggtcagcagc acgccggaat
      901 tgtcggcatc gatcgcctgg cgcgcggcct tgatgcgttc gatcgcatgg gcgcgctcgt
      961 aaagcggctc tgcggcatcc ccggtggaat cctcgatcga cagaccggcc acgccggtct
     1021 tgaccgcacg cgcgacattg acgccaacct tctgcgcctc atgggcaaag ccgccctcga
     1081 aatcggcgtt gacgggcaga tcgaccgccg cgcacagcgc ggtgagatgc gtacagacct
     1141 cgtcgagcgt gacgcgattg tcggccttgc cgatcgtcca cgcaaagccc gcactggtgg
     1201 acgccagcgc cttgaagcct aagtgctgca gcgccagcgc gctgccgacg tcataaggat
     1261 tgggaatcgc gaaacaaccg gtttcgtgca tcttgcggaa tgtcgctcgt ttttctgctg
     1321 ttgtgaccgg catgatcgct ccctgttttt atgttcggta agttagttac atgacgatag
     1381 tcaccgggcg cgcgctgcgc tagtgcgcgt cgtgcgtggc ccggctgtcc tttggcgcct
     1441 gcgcgcgatc tgtcatgctg tagtccctga tgatgccggc aatacgcagc cggtaattat
     1501 cgaaaatcct ggcgcgccct ttggcctggg ttttgcgatg ctggggaatg ttgcgccatg
     1561 ccgcgacggc ctcctcgtcg cggaagaacg acagcgacaa gatcttgcct ttctcggtca
     1621 ggctttcgaa ccgttcgatc gagatgaagc catcgatggt ttccaggatc ggccgcagtt
     1681 cggccgcgag gtcgagatat tctcctctgt actccggctt gggccagacc tcgaagatca
     1741 cggcgatcat ttgcgtctcc tcctttgcgc tgggatgttt tatgttggga tgttttataa
     1801 cgtcaccttg cgcagaaatg ttcgttcctc cgcaaggatg aatttgttgt cttcggcgaa
     1861 attgaaattg gccatgccct cctggtcggc gcgaagtctt gcgcgataag tttcataggc
     1921 ggcgagactc tcgaacgaaa tcagcgcgaa cgcaatattg ttggtgccct catgcggcat
     1981 ccagtagccg acgagatcgc cgccgcattt cggaatgatg gtcagccagc gcttggcgta
     2041 ctcctcgaac atcgcgcgct tgaacggatc gagctggtag cggatgaaaa cggtgatggc
     2101 caaagggaac ctccggggtt ttgagcgctc ccaaaatagc cacttgacga cgtgaatgct
     2161 tcggctatca tcgaactatg aaagcaggcc ccgacatcgc catggtcgca gcactggtcg
     2221 gcgatcccgc gcgctccaac atgctgacgg cgctgatgac cggccgcgcg ctgaccgcga
     2281 gcgagttggc gcaccaggcc ggcgtcaccc cgcagaccgc gagttcacat ctttccaagt
     2341 tggaggccgg cggcctgatc gagccggaga agcagggtcg ccaccgttac tatcgcctca
     2401 ccggcccgga tgtcgccggc gtgctcgagg ggcttgcggg tcttgcggcg cgcgccggtc
     2461 acatgcgcgt gcgcaccgga ccgaaggatc cggcgctgcg ccgcgcgcgg atttgctacg
     2521 atcatctggc gggcgacctt ggcgtgcaaa tgctcgacag cctgataagg cagcggctgg
     2581 ttcgccagag caagcaggcg atcgaactca ccgccgaggg caagcgcttt atggaaaaga
     2641 gcctgcagat atcagccgag gcgctggccc atccgcgccg tccggtgtgc aaggcgtgcc
     2701 tggactggag cgagcgacgc catcatcttg cgggcacgct gggtgctgcc atgatgacgc
     2761 ggttcgccga actgaaatgg gccgcgcgcg attccacccc cggcagccgc gtcgtcaatt
     2821 ttacccgcaa tggcgagaag cggttcgccg agctgtttgg gaacgacgag tagccgggcg
     2881 gagagatttc actactgcaa cttcaacttc ccgtcatggc cgggcatgac gaacatgaag
     2941 gttgggcttg attcgatgcg acgatttttc tttgtgatct ctgcggcggt ccttgcgttg
     3001 ccgttctccg ccaacgccgc ggagcccgcc gcgcgcgaac cctacggcat cgggctggag
     3061 ggttttgcct atccctaccc cgtcaacatg ctgccgctta gcaatgacgg cgagcaggtg
     3121 cggatggcct atatggatgt cgcgccgggg cagccgaacg gccgcaccgt ggtgctgctg
     3181 cacggccgca attttccgtc gagctactgg gcgcctgtga tcaagacgct gaccgacgcc
     3241 ggctaccgcg tggtggtgcc ggaccagatc ggctttggca aatcctcaaa accgtccgcc
     3301 gacctgcact tcgatacgtt ggcgcgcaat accatcgcgc tgctcgatca tctgcaaatc
     3361 gcaaaggccg agttcgtcgc gcattcgctg ggcgggatgc tcggcgtgcg gatcgcacgc
     3421 gcctatccgg atcgggtcgc gcatctgttg ctcgcggccc ccatcgggtt ggaagattac
     3481 cggctctacg tgccgccgac gccaaccgaa aagattctgg agaacgagga cagactgacc
     3541 gccgacggct accgcaagca gcttcagacc aattattcgt taaaactgcc gcccgaccag
     3601 gtcacgccgt tcatcgacgc gcgcttcaac atcaagggca gttcggaata tccgcgctgg
     3661 ctgcgcgcct ttgtcagctc ggcgcaaatg atctatcgcg agcccgtggt gcacgaaatt
     3721 ccgctcatca cgcgaccgac gctgttcatc atgggcgccg acgatcacaa cgcgcccggc
     3781 aaaccgaatg caccggaagc gctgcggcca aagatgggcc aaaacgccga gctcgccaaa
     3841 gcgctggcgg gacaaatgcc cgacgccaaa gccgaagtgc tcccgaacgc cgggcatctg
     3901 gtgtttctcg atgcgccggc aaaattcaac gagctgatgc tggcgtttct ggcgaagtga
     3961 atgctggcga acgaaacgtc gaggcaggga ccaggaatgc ggtccctgcc tggcgcaacc
     4021 gctaccagtt tctaaagcaa tgccggccga acggtccgga atgccagccg ggcggacaat
     4081 tgtacatcgg gcggcatccg ccataggggc cgcggtgcca gccgggaccg cagccaccgg
     4141 cgacgcgaat ggtgtcggac gaaacggact ggtcgagcgg ggccagcggc accgcctggg
     4201 atgccgacat cgccatcagg ctgaggccga cggcagcggc agaagcggtt agcagtttca
     4261 tgaacttttc cttcctttcg tggttcgccg ttaggcggca gatttcaatt cacaaactat
     4321 ccagcgatcg ggcaacgccg ggccgcccca ccagtgacgc ccgacgccag tagcagtggc
     4381 gatgccaacc cgatgactct tcacatcgag cgtgacgaag agagttttgc tgagtcgaga
     4441 tgaatgttcg ctgaactgga tcgcgtaggt gcaacgcgca taacaccagc gtcgtcccgg
     4501 cgaacgccgc gaccccagcg tgaacgcaat tgcgttcatc gcggcggcgg tgcggactca
     4561 cgcgtgaagg atggagcgga tatcacgcgc aagattaacg ccggtgttta tgggtcccgg
     4621 ccttcgccgg gacgacactg cctttgcgat gacaaaggct catccttcat cccgcactga
     4681 attcttcggc cagcaccaaa agctcgcgcg tgcgggtgat cccgggccag tcgcgatcaa
     4741 agtcggccac cagccgtttc aggtcactgc cgttcatcgc ccggtccagc gcggccttgt
     4801 cggagaattg gtaggtcgcc tgatgcagcg cgggatcgct gaggctccag aagcgccagg
     4861 ccttttcgac gccgaacgat tttgcggcgt cgggaagatg ctccttgccg taccaccggt
     4921 cgaaggctgc ccgtttggcg ggatcggaga cggtggcgcg aacgacgaaa taggctgctg
     4981 gcattggctt cctccgcaag tttgttcatc tggttgtaag gcaggtcagg cgcagccgta
     5041 acccaccgcc gcagtaacct actaaaccca tccgccgcgc gccatgatcg cggcgcacag
     5101 cagaatgatc acgatgctca aaagttcgcc gtgtataacg ccggtaatca atcgcagttt
     5161 cttggcgctg atcacgggca cctgtccggc accgagcgcg ctgcgccatg acagaaattc
     5221 gaccgtcggg atgatcgaca acagcccgat cgcgataaag atcgaaaatt ttgccgtgaa
     5281 ggcgtggctg tgaaaataat agtccgcacc cttctcgaaa taaaataccc gtaacgaccc
     5341 gacgatgaac agcgcgccgg cggcgatgcc gagcaccgta tcggtaacct gcaacctgcg
     5401 cgccgaggaa agcgtcagct cctgatggac cagcgtgaac tcgatcgcga ccgccgacac
     5461 cagcgtgaag gcgcagaggt ggtggaggaa agcgaacagc gtggacatgg cgcgggctcc
     5521 ggctttccgg gaaatgtaga cgacggaaaa atggacgcaa gcctgccgaa gaggcgcgag
     5581 gagaacttcc gctcgtcatt ccggggcatc gccaacgggt cggcgctctg cgccgcccga
     5641 tgacaggctc cgcgatgagc ccggaatcca taaccaccgc cgggagtatg gattccgggc
     5701 ctgcgcgtcc aagtcggctg ttgccgactt ggacaatgac attgccgaac tcgggtttac
     5761 cctgggtaaa cccgagttcg gtggcgcatc ccggaatgac gagagtccta cgcgttcgac
     5821 accgaagtcg tactcacaac ggcaaattat cgtgcttctt catcggcatc tcgacatgct
     5881 tgtcgcgcag catcgccagc gcccgtgcga tgcgtcgcct cgtcgagtgc ggcatgatga
     5941 cgtcgtcgat atagccgcgc tcggcggcga cgaagggcga caggaagcgg tcttcatact
     6001 ctttcgtgcg cccggcgatc gcttcggtgt cgccgatgtc gctgcggaag atgatctcga
     6061 ccgcgccctt ggcgcccatc accgcgatct gcgcggtcgg ccaggcgtag ttcatgtcgg
     6121 cgccgatttc ctttgacgcc atcacgtcga acgcgccgcc ataggccttt cgcgtgatta
     6181 ccgtgaccag cggcaccgtg cattgcgaat aggaaaacaa cagtttcgcg ccgtgtttta
     6241 tcagcccgcc atattcctgg gcggtgcccg gcaggaaacc gggcacatcg acgaaggtca
     6301 cgatggggat gttgaaggca tcgcagaagc ggacaaaacg cgccgccttg cgcgaggcgt
     6361 ccgagtcgag cacgcccgcc agcaccattg gctggttggc gacaaagccc acggtgcgcc
     6421 cggcgatgcg cccaaacccg gtgatgatgt ttttcgcaaa ggtttccgaa atctcgaaga
     6481 aatcgccctc gtccaccact ttgaggatca gttccttcat gtcgtagggc ttgttcggat
     6541 tgtcggggat cagcgtgtcc agtgacatgt cgacccgacc gatgtcatca aagctcggcc
     6601 attccggcac gccgtcggta ttgttgctgg ggagaaaatc gatcagccgg cgcatctgca
     6661 acaaggtctc gacgtcgttc tcgaacgcgc cgtccgcgat cgaggagcgc gtggcgtgca
     6721 cggatgcgcc gccgagttct tcggcggtca cgacctcgtt ggtgacggtc ttcacgacgt
     6781 cggggccggt cacgaacatg taactggtgt tcttcaccat gaagatgaaa tcggtcatcg
     6841 cgggcgaata gacgtcgccg ccggcgcacg gccccatgat gaccgaaatt tgcgggatca
     6901 cgccggatgc gatgacgttg cggcggaaca cgtaggaata gcccgccagt gccgcgacgc
     6961 cttcctggat ccgcgcgccg ccggcgtcgt agagcccgat gatcggcgcc ctcgccttca
     7021 tcgccatgtc ctggatcttt acgatttttt gcgcgtgggt ttcggacagc gagcccccga
     7081 acacggtaaa atccttggcg aacacaaagg tcttgcggcc gttgacggtg ccccagccgg
     7141 tgacgacgcc atcgcccggc acccttgtct tttccatgcc gaattcgacc gagcggtgct
     7201 cgacgaacat gtcgaactcc tcgaacgagc ccttgtcgag cagcagctcg atgcgctcgc
     7261 gcgcggtcag cttgccgcgg gtgtgctgcg cctcgatgcg cttttcgccg ccgccgagcc
     7321 ttgcgccggc gcgccgttcc tcgagggtgt ccaggatctc tttcatgtga tgctttcccg
     7381 tcaaaatttg cccgggttct aacacggcat tttgccggcg ggaaacgccc ctccggcatc
     7441 tggcacccct gccccagcga cgatatatcc cgtgcctcaa cggaggcgac gtttatgacg
     7501 gatatcccgg gcgcggaaac aaccggcgcc gtaacaggcg gattacgcac cctgctccgg
     7561 ctggaagggc tcacgctgtt taccggtatg acgctgcttt acgcggtctg gggcggatcg
     7621 tggtggatct acgccattct gttcctggtg cccgatctca gcttcgcggc ctatctgtgg
     7681 gggccccggc taggtgccat catctacaac gcggcgcaca gctacatggc cccgatgacc
     7741 ctgatgaccg gcgggtttgc cttggcctcg ccgctggtgc tttcgatcgc gatgatctgg
     7801 ctcgcgcata tcggcatcga ccgcgcgctc ggctatggcc tgaaatacaa tgcgggattt
     7861 ggcttcaccc atcttggccg tatcggcaaa gacgcggtaa agacctgatc aaattggcag
     7921 cccgcccgcg acttgttcta ttcgttggga cgatggtgtc ccggcaaccg cagtgcgctc
     7981 cctctgccct tttgggagag ggagcggtgt ggcagttgtg taggcgctaa tctaggttac
     8041 ccggtggctc gcgatatcga ccgtgacttt atcgaatggt gtcttcacgc cattggtcgt
     8101 ctctaccatt tcgatagcaa gctgcgcggc gttcgcggtc atcgtgagat agccgaaccc
     8161 ttttagcgac ttttcaaaaa tcgtttgccc catctgttgc cccgttgcag ccgtgacggc
     8221 tgaatcagcg tgtccgctgc atcctgcaac aagataggga atctgcacct gcgcgccagc
     8281 cagtgccgcg gtgcgcgtgt agcgctgata gttatgcgaa tggcccgcga gcatcgcgtg
     8341 cggcacgacg ccggcatcat tgcaggcctt gtcaatttgc gcgagcatgt ccttgctcgg
     8401 actatggccg ccggagctgt aaggcggatg atgcgtcgcg atgaccaacg ccttcttgtc
     8461 acctgactgc ttgagacttg tcagcgtctt tgtcagccat ttgatctgct gatcgtctcc
     8521 gttcgcgccc agcaaattac cgggaccttc agcgatattg gaatagaggc cgatgatgtt
     8581 cgcaaacggc gccgacagca tccagtaaac gccgggctgt gtcatcgttt cccggaaaat
     8641 gcggacgtct cccgcgatgc tcggaaccag cgcgcttggc gcgcaaaaat tgttgcgaaa
     8701 ctcctgcagc gagaccggat cggtgccctt gaatgtttcg ccatcgtgat ttccgggaat
     8761 cgcgatgatc tttccgggat acttcatgta ggggcgatag aactcgtcgc ggtattgctt
     8821 ggacttgtcg tgtccgtaga tcacgtctcc gagatgaatg agcagcgcgg gattatgtcc
     8881 ccccgctgcc ggatcgtagt cgcccgtcat ggcctgagcc acctgctctt gcggagagtt
     8941 gtctcctccc tgccggccgg tatcgccgat gacgtgaaac ttgatggcgc cggcttgctg
     9001 gatttcatcc gtccccggct ggccgatgat gtctttcagc tccatgagcg ggtcgcgtgt
     9061 cggccgcggg acgatgccaa gcttatccga agcgaactgc gccaggcttg tcgtactcgt
     9121 ccgcgcgcta accgctcggt ccggaacggg tgtcgtggtg aaaaatggat gggcaaatcg
     9181 caacggtgtt gcacgcggcg tgcgtccccc ggaacgggcg gtcttttttg cggtgcgctt
     9241 tgcagtcttt gccttctttg cctttttggc catgtcaatt tcctccggaa acaaattgtc
     9301 gagcaataac gcctgtcaca gtttgaaata tcgaagtcca ccttccgctg agcctgtcga
     9361 tccaatcgcc ctgcacccgc aaaatcagca acggccgcag ttccagcact atcccgctgg
     9421 cgcttgtccg ccatgcgcag cgccggccgc gatcgcctcg ctgagctcat atccaatacg
     9481 ccgcgcgcta aagtcgcttt gatggagccg ggtgatgtcg gtggggtcaa tgatggtgtc
     9541 gaacgaatgt ctttcgacat cgagaaattt ccgcatcagg tcgaaccggt gaaacacgtt
     9601 cacattcacg gcagtcgcga gctggttgat cagcgccagc atgcgccgcg ttgcgtcgat
     9661 cttgtcgtcg gtgagcacag cgggcgcata ttggaggtcc atcatgacga catccatcgg
     9721 ctcgccgccc agtaatttca ggcctttctc gatcgccgcg gcaacatcgt caaggttgtg
     9781 gcctggttgc cagacggcgt ttgtgccgac ctgccagatc gccagcgccg gggcctcggc
     9841 aatcacatgg tcgtgcaagc gctcgagctc ttcaggggct tcttgacctt ttacacctct
     9901 gttgagcacg tcgatcatgc ggttcggata cttgtctttc agggccgtct ccagccggta
     9961 tgtaaagggt acgatgtcgc cttcacctat tgtcgatgat gatccgattg cgacgatctt
    10021 gatcgagcct gagccgttga gccggttggc aaagtttgga aggcgatacg ggaaggcttc
    10081 gacgtgggcg gggatatagc ggtttggctg ctgatcaggc atttgaacca cgcgttcgca
    10141 gggcgcagcc agcaattgct gtcgcctcat atgtcggcac cggacccggc gctcatccaa
    10201 aaaaatcaat cagcgagctt ggccggccgg gtaaccggcc gtctgatgtc gataataatt
    10261 acgctcttgt ttcattcatt caaaacaatt caagtctaca atcttcgatt gaaagagtcg
    10321 aggccgcgat cggcgcaaga caattttaat tcgccatggg cgcccaggga atcatgggcc
    10381 gccagcgaat aaagtaccct ccctgaccgg attgcgcctg atgatgcgga gccggtcatc
    10441 gggcaggcat tcgcgcggcc cattggcgtc acctgtgcga tccgacctct cccacaaggg
    10501 gagcggtgac accgagcaga gcgcccggcc tacttctccc cgaccttgaa cggcggctcc
    10561 ttgccgggag gcgtggtttc ctccgccatg gccagcatca tggcgaccat gacgtagttg
    10621 ccggccaccg ccgtcagatc cacgatctgc tgatcgctga aaaccttctt ggcccgcgca
    10681 taggtttcgt cggaaacctt ttgcgtggtc gtgagctcgg tgacgaaatc gtaaaccagc
    10741 gcctcgtcgt cggccatgcc ggacggccgc ttgctggcct tcagctcggc gatgatgtcg
    10801 ggcgataatc ccgccttcgc cgccagcggt gcgtgtgcgt accattcgac ctgcgagcgc
    10861 cactgccggc cgatgatcag gatcgcgaat tcgttgagct tggtcgggac cgaggtttcc
    10921 cagcgcaggt agtgaaacag gtcgaacagc cgctggccca gcaccgggct gcgcaacatc
    10981 gggttatacg gcccggccag cccgacgctg gaaaccttca tcacctgctc gccaagc
//
LOCUS       DASWGM010000151         6857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2644495,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000151 DASWGM010000000
VERSION     DASWGM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2644495"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VGA15_04620"
     CDS             <1..561
                     /locus_tag="VGA15_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587009.1"
                     /translation="KRRVREYPHQLSGGMRQRVMIAIALACSPKLLIADEPTTALDVT
                     IQAQILHLMLDLKRRVGAAIVLITHDLGVVAEIAERVMVMYAGRKVEEAPVAELFRSP
                     RHPYTQGLLGAVPKLGSSLTGAARRLAEIPGQVPSLKKRIEGCVFAGRCSLATDLCRE
                     VAPGLEEKGPRHIAACHYAPKEAAAA"
     gene            558..1565
                     /locus_tag="VGA15_04625"
     CDS             558..1565
                     /locus_tag="VGA15_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9587010.1"
                     /translation="MSAPLLQVNDLKKHFPVRGGLFSRKSSWVYAVDGVSFEVERGET
                     LSLVGESGCGKSTVGRAILRLFDITAGQVVLDGQRIDDLSPGGLRSMRRRVQVVFQDP
                     FSSLNPRMRVRDILAEPIRNFGLAKSSAELEARVAALLDTVRLPRDALNRRPHEFSGG
                     QRQRIGIARALAAEPELIVCDEAVSALDVSVKAQIVNLLQDLQAKFGLALLFISHDLA
                     IVEHMTHRVAVMYLGKIVEMAPRQQIFTAPKHPYTRALLSAVPVPEPGAARTPMILKG
                     DVPSPINPPSGCRFHTRCPFVFDRCRTEEPKLRSTGDGQWVACHLNALPEERDPRGDP
                     R"
     gene            complement(1630..5838)
                     /locus_tag="VGA15_04630"
     CDS             complement(1630..5838)
                     /locus_tag="VGA15_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9587011.1"
                     /translation="MNESALANRPRAASAIPVAPGYALHTAEQAYRRVLADQPGHFRA
                     LCGLAVVRGQLGALDEARDLIGQAVDIAGQSAEDHVLLGTTFIRMNDLASAHRQFEMA
                     VALDEGHAAARFHLANVLSGRGDVADAVIHYERVLAIDPDNADAHQNLGLALQRIGQF
                     EAALAHHQAALAISPRSAAIHVSLGDACRHLGRHDDAIVQYARALAINSGLTDAHINL
                     GGCFHATGRREQAIRSYQRALTVNPGLADAHYNLGNLFVELDNLDAAIVHYERSIALR
                     PASAEAHNNFANVLQKRGRYEEAVAHYKDAIRLKPTYVSAQRNLGDALQAQGSIDGAI
                     VCYRAALVIEPRDSTTLNRLASVLLVAGRLDEASRAYETAIDIAPENIGLQHNYAGVK
                     PFADGDVRLTRLEELHARKDQLSDAERIVLNFTLGKAYADLKDPDRSFRHLDAGNRLK
                     RQHVTYDERGTLMLMQRIGDAFTRDVLRARSNVGHDSEAPVFVIGMPRSGTSLVEQIL
                     ASHPRVFGAGELNDFVATMSDVVERRAGAYPDMLAKMSDEDFGELGKTYVERVSRIGA
                     AKDRIVDKLPSNFLFAGLIHLALPRARIIHVKRNAVDTCVSCYSLLFAEDQPFAYDLG
                     ELGRYYKAYEALMDHWRSVLPPERMLEVRYEDLVEDLEGQARRLVAHCGVEWDKRCLA
                     FHETKRPVHTASLVQVRKSIYASSVGRSRLYGSRLKPLVDALEIEDTPSKAEQGATAG
                     LQKRRSNQRAAAENFSEPPAPADRKAEIGKPEASTELAFRVAKKLQERGDLSDAEPLF
                     RVVLAMRPDHFGSLLALGSICALTNRLDDAKRYLTQAIAANKDAAEAHGSLGAVHTSA
                     GDLNAAAACYDKALALAPDHPGIHYAYAMLLQSLGRSAEAIDHLRRAVSGRPDHIDAH
                     FALGNLLYAGGQDAEAIECYFKVLQFNPRHPETHNNLGNVYQRQGQMERAITHYKTAI
                     EINPSYPDAYGNLGNAYLVLDRLEESIAQNRRALELKPSRFGSHNNQGVAFQALGRFE
                     EAERAFERALELEPREASVHLNLANMGRFKAGDRRLPGLKKLLNEVESLDADNQIAAH
                     FAVGKALGDLGQYQDAFRHLLKANALKRRDIIYDEPQRLAMFENIRTTFSQDFIKARS
                     GVGDTSWSPIFIVGMPRSGTTLMEQVLASHSKVFGAGELETFKEALAECVSSQGIVPA
                     YPALVEALSPDHIHQLGEIYTTRVCARAPEAERIVDKMPLNFVFVGLIHLALPNARFI
                     NVRRDPLDTCVSCFSLLFTGSQPFAYDLAELGRYYRGYETVMEHWHKVLPPGVMIDVH
                     YEDLVDDLEGTARRVLRHCDLDWEDACRDFHDTKRAVRTASLMQVREPVYRRSIGTWR
                     RYAEFLEPLGQALGVDVRARLADT"
     gene            complement(5952..>6857)
                     /locus_tag="VGA15_04635"
     CDS             complement(5952..>6857)
                     /locus_tag="VGA15_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587012.1"
                     /translation="MLNQINLQLTKYDGAEANGSATALRGTSDNVLDIIDIVQGDANL
                     NMAAGGNGAAGHAGGFAEMPGGLTGTVTKFQDNQAQTNFWASFLAEANTINAHLTAIA
                     GGGEQASAALITQIQNYQQFGANFDAAQGAIFQGRFDNELNNGTLEADTAAAIKGLTG
                     ILNGDTGAALAADHAMITAAGQGFAADAMDVSGNNIAIGGATYVGTATTVATATSVNG
                     LAQGSIPVTATPNLANGTGGAATSGTTTSGPGGTPPAGPCGDPNHGHDDAPAAAPAAA
                     ALAPVGIHMPTPIEAFHQHFDHMWG"
BASE COUNT         1233 a         2156 c         2141 g         1327 t
ORIGIN      
        1 aagcggcgcg tgcgcgaata tccgcatcag ctctccggcg gcatgcgcca gcgcgtgatg
       61 atcgccatcg cactcgcctg cagccccaag ctcctgatcg ccgacgagcc gaccacggcg
      121 ctggacgtaa cgatccaggc gcagatcctg cacttgatgc tggacctcaa acgccgcgtc
      181 ggtgcggcga tcgttctgat cacccatgat ctcggcgtgg tcgccgaaat cgccgagcgc
      241 gtcatggtca tgtatgccgg ccgcaaggtt gaggaggcgc cggtagccga gctgttccgc
      301 tctccccgtc atccctatac gcagggactg ctcggcgcgg tgcccaagct cggctcctcc
      361 ctcaccggcg cggcgaggcg cctcgccgag atccccgggc aagtgcccag cctcaagaag
      421 cggatcgagg gctgcgtctt tgccggacgg tgttccctgg ctaccgatct ctgccgtgag
      481 gtcgcgccgg gtctcgaaga gaagggtccc cgccacatcg ccgcctgcca ttatgcgccc
      541 aaggaggcgg ccgcggcatg agtgccccgt tgctccaggt caacgacctc aagaagcatt
      601 ttccggtccg cggcggcctg ttcagccgca agtccagctg ggtctatgcc gtggacggcg
      661 tgtccttcga ggtcgagcgt ggtgagaccc tgtctctcgt gggcgagtcc ggctgcggca
      721 agtcaacggt cggccgagcc atcctgcgac tgttcgacat caccgccggc caggtggtgc
      781 tggatggcca gcgtatcgac gatctttcac ccggcggcct gcgcagcatg cgccgccgcg
      841 tgcaggtggt gttccaggat ccgttctcca gtctcaatcc gcgtatgcgc gtccgcgata
      901 tcctggccga gccaatccgc aatttcggcc tcgccaagtc ttccgccgaa ctcgaggcgc
      961 gggttgcggc gctgctggac accgtgcgcc tgccacgcga cgcgctgaac cgccggcctc
     1021 acgagttctc aggcggtcag cgccagcgca tcggcatcgc ccgggcactc gcggcagaac
     1081 ccgaactgat cgtctgcgac gaggcggtct cggctctcga cgtctcggtc aaggcgcaga
     1141 tcgtcaacct gctgcaggat ctgcaggcca agttcggcct cgcgctgctc ttcatcagcc
     1201 acgatctggc gatcgtcgag cacatgacgc accgtgttgc agtgatgtat ctcggcaaga
     1261 tcgtcgagat ggcgccgcgg cagcagatct tcaccgcgcc gaagcatccc tacaccaggg
     1321 cgctgctctc ggcggtgccg gtgccggagc ccggggccgc ccgcaccccg atgatcctga
     1381 agggcgacgt gccgagcccg atcaatccac ccagcggatg ccgcttccac acaaggtgtc
     1441 cattcgtgtt cgatcgctgc cggacggagg agccgaagct tcggtcgacc ggagacggtc
     1501 agtgggtggc gtgtcatctc aacgcgctgc cggaagagcg cgacccgcgc ggcgatccac
     1561 gctaggcgcc gccggatatc gataccggtc gaatgaggat tccatccatg cgatttggca
     1621 gcaggcgggt tacgtgtccg ccaacctggc gcggacgtcc accccgagag cctgccccaa
     1681 gggctccaga aactccgcgt atcgacgcca ggtgccgatc gagcgccgat aaaccggctc
     1741 gcgcacctgc atcagactgg ccgttcgcac cgcccgtttt gtgtcgtgaa aatcgcggca
     1801 cgcgtcttcc caatcgaggt cgcaatggcg cagaacgcgt cttgcggtac cctcgagatc
     1861 atcgacgaga tcttcgtagt gtacgtcgat catgacgccc ggcggcagga ccttgtgcca
     1921 gtgctccatg accgtctcgt agccgcgata gtagcggccg agctcggcga gatcgtaggc
     1981 aaagggctgg cttcccgtaa atagcaacga gaaacacgag acgcaggtgt cgaggggatc
     2041 acgacgcaca ttgatgaagc gggcgttcgg cagcgccaga tgaatcagcc caacaaagac
     2101 aaagttgagc ggcattttgt cgacgatccg ctcggcctca ggtgcgcgcg cgcacacccg
     2161 cgtggtgtag atttcgccga gctgatgaat atgatccggt gacagggcct cgaccagcgc
     2221 gggataggcc ggcacgattc cctggctgct gacgcactcc gcaagcgcct ccttgaacgt
     2281 ctccagttct ccggcgccga acaccttcga gtggctcgcc agcacctgct ccatgagcgt
     2341 cgtcccggag cggggcatac cgacaatgaa gatcggagac cacgaagtat cgccgacgcc
     2401 cgatctcgcc ttgatgaagt cctgcgagaa cgtcgttcga atgttctcga acatcgccag
     2461 cctctgcggt tcgtcataga tgatgtcgcg ccgtttcagg gcattggctt tcagcaaatg
     2521 ccggaaggca tcctgatatt ggccgagatc gccaagggct ttgcccaccg cgaaatgcgc
     2581 agcaatctga ttgtccgcgt ccagggattc aacttcattc agcagcttct taaggcccgg
     2641 cagacgacga tcgcccgcct tgaaccgccc catatttgca aggttcaggt gaacggacgc
     2701 ctccctcggt tccagttcga gcgcccgctc gaaggcgcgt tcggcttcct cgaaccgccc
     2761 caacgcctgg aacgccacac cctgattgtt gtgcgatccg aaccttgaag gcttcaactc
     2821 aagggcgcgc cggttctgcg cgatcgattc ctcgagacga tcgagcacaa gatacgcatt
     2881 gccgagattg ccataggcat cggggtagga tgggttgatt tctatcgccg ttttatagtg
     2941 agtgatcgca cgctccatct ggccctgccg ttggtacaca ttgcccagat tattgtgagt
     3001 ttccgggtgg cgcgggttga attgcaatac cttgaagtag cactcaatcg cttcggcatc
     3061 ttgtcccccg gcataaagca gatttccgag cgcgaaatgg gcatcgatat gatcgggcct
     3121 tcccgaaaca gcgcgcctga gatgatcgat ggcttctgcg ctcctgccga gagactgcag
     3181 gagcatcgca tacgcgtaat ggatcccggg atgatcaggc gcaagcgcaa gggccttgtc
     3241 gtagcaggcc gccgcggcgt tgaggtcgcc tgcgcttgtg tggacggcgc ccaggctccc
     3301 atgtgcctcg gcagcgtcct tgttagctgc gatcgcttgc gtgaggtagc gtttcgcatc
     3361 gtcgagccgg ttggttaagg cgcatatcga gcccaacgcc agcagtgaac caaagtgatc
     3421 cggacgcatc gcgagcacga ctctgaagag cggctccgca tcgctcaggt cgccccgctc
     3481 ctgcagcttc ttggccactc gaaaggcaag ttctgtcgaa gcctctggct tgccgatttc
     3541 agccttccga tccgccggag ctggcggttc ggaaaagttt tccgccgccg cgcgttggtt
     3601 cgagcgccgc ttttgaagcc cggcggttgc gccctgctca gccttggaag gagtgtcttc
     3661 gatctcgagt gcgtccacga gcggcttgag ccttgaaccg taaagtcgcg atcgcccgac
     3721 ggaactggcg tagatcgatt ttcgcacctg aaccagactt gccgtgtgga ccggacgctt
     3781 agtctcgtga aacgcgagac agcgtttgtc ccactccaca ccgcaatgcg ctacaagacg
     3841 tcgcgcctga ccctccagat cttcgacgag gtcctcgtat cggacttcaa gcattcgttc
     3901 cggcggcaga acggaccgcc agtggtccat caacgcttcg taagccttgt agtagcgacc
     3961 gagttcgccg aggtcatagg cgaatggctg gtcttccgcg aacagcagcg agtagcatga
     4021 tacgcaggtg tcgacggcat tccgtttgac gtggatgatc cgggcgcgcg gcagcgccag
     4081 atggataagg ccagcgaaca ggaaattaga cggcaatttg tcgacgatgc gatccttcgc
     4141 tgccccgatc ctgctcacgc gttcgacata ggtcttgccg agctccccga agtcttcgtc
     4201 cgacattttg gcgagcatgt ccgggtacgc gcccgctctg cgctccacga catcggacat
     4261 tgtggccaca aaatcattga gctctccggc tccgaagacg cgcggatggc tggcgaggat
     4321 ttgttcgaca aggctcgtgc ccgagcgcgg catgccgatg acgaagaccg gcgcctccga
     4381 gtcatgtccg acattggatc tggcgcgcag gacatcgcgg gtgaatgcgt cgccgatgcg
     4441 ctgcatcagc atcaaggttc cgcgctcgtc atacgtcacg tgctgccgct tgagacgatt
     4501 gccggcgtcc agatgacgaa acgaacggtc tggatccttg aggtcggcat aagcctttcc
     4561 gagcgtgaag ttcagcacga tccgctccgc gtcggaaagc tgatccttcc gggcgtgaag
     4621 ttcttccagc cgggtcaggc gaacgtcgcc gtccgcaaac ggcttgacgc cggcgtagtt
     4681 gtgctgaagg ccaatgtttt caggcgcgat gtcgatcgcc gtctcgtagg cccgcgatgc
     4741 ttcgtcaaga cgccccgcga ccagcaacac cgatgcaagg cggttcaggg tcgtcgaatc
     4801 ccgtggctcg attaccagcg cagcccgata gcacacaata gcgccatcga tactgccctg
     4861 ggcctgcagc gcatcgccca aattgcgctg cgcggataca taggtcggct tgagacgaat
     4921 tgcgtccttg taatgcgcga cggcttcttc gtagcgacca cgcttctgca gtacgttggc
     4981 aaaattgttg tgcgcttcgg cggaagccgg acgcaacgcg atggaacgtt cgtaatgaac
     5041 gatcgccgcg tcgagattgt cgagttcgac gaacagattg ccgagattgt agtgcgcgtc
     5101 tgcaaggccg ggattgaccg tcaacgcccg ctggtagctg cggatcgcct gctcgcgacg
     5161 gcccgttgca tggaagcagc cgccgaggtt gatgtgtgcg tccgtcaagc cggaattgat
     5221 cgcgagcgcc cgcgcatact gcacgatagc atcatcatgc cggccaagat gccgacaggc
     5281 gtcgcccagg ctgacatgga tggcggcaga tctcggcgag atcgcgagcg ccgcctggtg
     5341 gtgggccagg gcggcttcga attgaccgat cctctggaga gccagtccaa gattttgatg
     5401 ggcgtccgcg ttgtccggat cgatcgcaag cactctttcg tagtggatca ccgcatcagc
     5461 aacatcgcca cggccgctga ggacgttggc cagatgaaat ctggccgctg catgcccttc
     5521 gtcgagtgcg acagccatct cgaactgacg atgggcgctc gcgagatcgt tcatccgaat
     5581 gaaggtcgtc ccgagcaaga catggtcttc ggccgattgc cctgcgatat cgactgcctg
     5641 accaatcagg tcgcgagcct cgtcgagcgc accaagctgg ccacgaacca cggccaaccc
     5701 acaaagggcg cggaagtgcc cgggctgatc ggcgagaacc cgtcgatagg cctgttcggc
     5761 tgtatgcaag gcgtatccgg gcgcaactgg gattgctgaa gcagcgcgcg gccggttggc
     5821 tagagctgat tcgttcatgg gcgtgccgtc tcatgagctg cgtttgccgg tcccttaaag
     5881 acgaaccagc gcagaacaaa gttctgcgct ggggagctct tacacaatgg caatttcgtc
     5941 gcgttggcgg cttaccccca catgtggtca aagtgttggt ggaaggcctc gatcggggtc
     6001 ggcatgtgga tgcccaccgg ggccagggct gctgctgccg gtgctgcagc tggggcgtcg
     6061 tcgtgaccat ggttggggtc gccgcaaggt ccggcaggtg gcgtgccgcc aggaccagac
     6121 gtcgtggtgc ccgaagtcgc cgcaccgccg gtgccattgg cgaggttggg ggtcgccgtc
     6181 accgggatgc tgccctgtgc cagaccattg accgaggtcg ccgtcgccac cgtcgtcgcc
     6241 gtgccgacat aggtggctcc gccgattgcg atgttgttgc cgctgacgtc catggcgtcc
     6301 gcggcaaagc cctggccggc agccgtgatc atcgcatggt ccgccgcgag cgccgcgccg
     6361 gtatcgccgt tcaggatgcc ggtgagcccc ttgatcgcgg cggcggtatc ggcctccagc
     6421 gtgccgttgt tcagttcgtt gtcgaaccgg ccctggaaga tcgcaccttg cgcggcatcg
     6481 aaattggcgc cgaactgctg gtagttctgg atctgcgtga tcaacgccgc gctcgcctgc
     6541 tccccaccgc cagcgatcgc cgtcagatgc gcattgatgg tgttggcctc ggccaggaac
     6601 gaggcccaga aattggtctg cgcctgatta tcctggaact tggtgacggt gccggtcaga
     6661 ccgcccggca tttcagcgaa gccgccagca tgccccgcgg cgccgttgcc gcccgccgcc
     6721 atgttgaggt tggcatcgcc ctgaacgata tcgatgatgt cgagcacatt gtccgaagtg
     6781 ccccgcagcg cggtggccga gccgttcgcc tctgcgccgt catatttggt gagctggagg
     6841 ttgatctggt tcagcat
//
LOCUS       DASWGM010000152         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_844961,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000152 DASWGM010000000
VERSION     DASWGM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844961"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VGA15_04640"
     CDS             complement(<1..912)
                     /locus_tag="VGA15_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9587013.1"
                     /translation="MDETMSEPVRTRPFAPFEWLLSGRYLRARRKEGFISVIAGFSFL
                     GIMLGVATLIIVMAVMNGFRKELLDKILGLNGHLLVQPLESPLTDWKDVAERISQVGG
                     IRLAAPVVDGMGLANSPFNASGVLIRGIRADDLNNLTSIAKNIKQGTLEGFDEGQGVA
                     IGRRLADQLSLHAGDSVTLVSPKGAVTPMGTTPRIKPYKVAAVFEIGMTEYDGSFVFM
                     PLAEAQAYFNRPNDVTAIEVFTTNPDKIDGFRKTVTEAAARPVFLVDWRQRNSTFFNA
                     LQVERNVMFLILTMIVLVAALNIVSGLI"
     gene            complement(949..2307)
                     /locus_tag="VGA15_04645"
     CDS             complement(949..2307)
                     /locus_tag="VGA15_04645"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX9587014.1"
                     /translation="MRLSRFFLPILKENPKDAEIVSHRLMLRAGMMRQEAAGIYAWLP
                     LGLRVLKKIEQIVREEQNRAGAIELLMPTLQLADLWRKSGRYDAYGPEMLRITDRHKR
                     ELLYGPTNEEMITEIFRSYRVSYKNLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
                     YSFDIDEAAARRSYNRMFVAYLRTFARMGLQAIPMRAETGPIGGDLSHEFIVLAETGE
                     SAVYCDSDVLDLPVPGEEIDYDGDLSPIIEQWTSVYAATEDVHDADRFDREVPAHKKV
                     QTRGIEVGQIFYFGTKYSEAMKAVVVGPNGVARPIHGGSYGVGVSRLVGAIIEACHDE
                     AGIKWPDAVAPFSVAILNLKQGLGETDRACEWLYQGLSTKGFDVLYDDTDQQAGAKFA
                     TADLIGIPWQIMVGPRTLAEGKVEIKRRSNGMRELISLTEVLAWLTHYNSSTELWRRY
                     EP"
BASE COUNT          509 a          738 c          726 g          498 t
ORIGIN      
        1 gatcaggccc gagacgatat tgagcgccgc gaccagcacg atcatggtga ggatcaggaa
       61 catcacgttg cgctcgacct gaagcgcatt gaagaaggtc gagttgcgct gccgccagtc
      121 caccaggaac accggccgtg cggcggcctc ggtcacggtc ttgcggaagc cgtcgatctt
      181 gtcgggattg gtggtgaaca cctcgatcgc ggtcacgtca ttggggcgat tgaaataagc
      241 ctgcgcctcc gcaagcggca tgaacacgaa gctcccgtca tattcagtca tgccgatctc
      301 gaacaccgcc gcgaccttgt agggcttgat acgcggcgtc gtgcccatcg gggttaccgc
      361 ccccttcggc gagaccaatg tgacgctgtc tccggcgtgc agcgacagtt gatcggctag
      421 tcggcggccg atcgcaacgc cctgcccttc gtcgaatccc tcgagcgtgc cttgcttaat
      481 gttcttggcg atcgaggtga ggttgttgag atcgtcggcg cgaatgccgc gaatgagcac
      541 gcccgaggcg ttgaacggtg agtttgccag tcccatgccg tccaccacag gcgcggccaa
      601 cctgatgccc ccgacctggc tgatacgctc ggcgacgtcc ttccagtcgg tcagcggcga
      661 ttccagcggc tgcaccaaaa gatgcccgtt gaggccgaga atcttgtcaa gcaattcctt
      721 gcgaaaaccg ttcatgacgg ccatcacgat gatcagcgtc gcgacgccca gcatgatgcc
      781 gaggaacgag aaaccagcga tgacggagat gaatccttcc ttgcgacgtg cccgcaaata
      841 gcgcccggac aacagccatt cgaatggcgc gaaaggtcgg gttcgaactg gctcgctcat
      901 ggtttcatcc atagatcgat tttcacacga ttcgggctaa ttgtggcgtt acggctcata
      961 gcgtcgccaa agttcggtgg atgaattgta atgggtcaac catgccaaga cctccgttag
     1021 actaatcagt tcgcgcatcc cgttgctacg ccgcttgatc tcaaccttgc cttcggcaag
     1081 agttctgggt ccgaccatga tctgccaagg aatgccaatc aggtctgcgg tggcgaattt
     1141 tgcgcctgct tgctgatcgg tgtcgtcgta tagaacgtcg aagcccttgg tagacaggcc
     1201 ctgatatagc cattcgcatg cgcggtcggt ctcaccgaga ccctgcttca gattgaggat
     1261 cgcaacagag aacggcgcca ctgcgtccgg ccacttgatg ccggcctcgt catgacaggc
     1321 ctcgatgatc gcgcctacca gccgcgagac accgaccccg taggagccac catggatcgg
     1381 gcgagcgacg ccattggggc ctacgaccac agccttcata gcttcagaat acttggttcc
     1441 aaaataaaag atctgcccga cctcaatgcc gcgggtctgt actttcttgt gggctggcac
     1501 ctcgcggtcg aaacgatcag cgtcgtggac gtcttcggtt gcggcatata ccgaggtcca
     1561 ttgctcgatg atcggtgaga gatcgccgtc gtagtcgatc tcctcgccgg gcaccggcag
     1621 atcgagcacg tcgctgtcgc aatagaccgc cgactcgccg gtctccgcga gcacgatgaa
     1681 ttcgtggctg aggtcgccgc cgatcgggcc ggtctccgcg cgcatcggga tcgcctgcag
     1741 tcccatccgc gcgaaggtac gcaagtacgc gacgaacatc ctgttatagg aacgccgtgc
     1801 cgccgcttcg tcgatgtcga atgaataggc atccttcatc aggaattcgc gaccgcgcat
     1861 gacgccgaac cgtggacgct gttcgtcgcg gaacttccac tggatatgat agagattgag
     1921 cggaagattt ttgtacgaaa ctctgtagct acgaaagatc tccgtgatca tctcctcgtt
     1981 ggtcgggccg tagaggagct cacgcttgtg ccggtcggtg atgcgcagca tttccgggcc
     2041 gtaggcatcg tagcggccgc ttttgcgcca gaggtcggcc aattgcagcg tcggcatcaa
     2101 cagttcgatg gcgccggcgc gattctgttc ctcacgaacg atctgctcga tcttcttcaa
     2161 cacccgcagg cccagcggca gccaggcata aatgccggcg gcttcctgcc gcatcatgcc
     2221 cgcccgcagc atcaggcgat gcgacacgat ctcggcgtcc ttcggattct ccttgaggat
     2281 gggtagaaag aaccgcgata atcgcatggg catactcaaa gaatcggtga aacacgggag
     2341 agccgcagtg aaaccggatc gggcccgaaa acacaagggc gcgcatcaac caaagccgct
     2401 aaggcagcgg atttcctgct gttttgccgg tgatcttaac cgggcgatta aggcgcgggc
     2461 gagaacaatc g
//
LOCUS       DASWGM010000153         5362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2507963,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000153 DASWGM010000000
VERSION     DASWGM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2507963"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1432
                     /locus_tag="VGA15_04650"
     CDS             227..1432
                     /locus_tag="VGA15_04650"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9587015.1"
                     /translation="MNTTSAAGLSKTRLRRMHDVMAGHVDDGSMPGLVSLVSRRGEVF
                     VDAIGRMAIGGAPMARDTIFRITSMTKPITAVAAMILVEECKLRLDDPVDRWLPELAN
                     RKVLRAIDAPLDDTVPARRAITLRDLLTFRLGYGMIMVFPDRYPIQKAIAEAGFAPGP
                     VFPSFPPDELMKRYGSLPLLYQPGERWLYNSGTEILGVLIARVAGTTFGEFLSDRIFA
                     PLGMNDTAFSVPEAKRNRFATCYVGDHATQSPKVFDDPVAGKFSSPPVFENGSAGLVS
                     TADDFNAFAQMMLNGGRIGSERILSRRSVELMTTDQITSEQKLGSELFFGENRGWGMG
                     LSVFTRRDDLYTAPGRFGWDGGYGTSWYSDPREQLTGLLLTQRMMDSPQPPRAFVDFW
                     TSVYQAIDD"
     gene            1579..1995
                     /locus_tag="VGA15_04655"
     CDS             1579..1995
                     /locus_tag="VGA15_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9587016.1"
                     /translation="MRFMVIVKADRNSEAGVMPSTELLTAMGKFNEEMVKAGVMLAGE
                     GLHPSSKGARIKFPGKGRTVSRGPFGLTGDLVAGFWLIQVKSLDEAIEWMKRAPFDDG
                     SEIEIRQVFDAEDFGEALTPELREQEQRLRAQAGKK"
     gene            2213..2629
                     /locus_tag="VGA15_04660"
     CDS             2213..2629
                     /locus_tag="VGA15_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9587017.1"
                     /translation="MRFMMLMIPLGYETAPPDVKLDPERVAAMMKYNQALKDAGVLIT
                     LDGLHPPSMGARVSFAGGKPVVTDGPFTEAKEVLGGYWMIDVKSREEAIGWAKRCPAT
                     GNEIIEIRQVQELSDFPADVQQAAAGLSELQGKPVR"
     gene            2708..3448
                     /locus_tag="VGA15_04665"
     CDS             2708..3448
                     /locus_tag="VGA15_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9587018.1"
                     /translation="MSWHKEQRRAERGYHHGNLKEALLQAALGLIAEKGAAGFTFADA
                     ARMAGVSPAAPYRHFRDRDELLSSIAQRGFEQFEVVLTNAWDDGRPDTVTAFERVGKA
                     YLAFARDEPAFYSAMFESGLPVDLNPTLLAASERAFGIIRAAAERLAAMAPPGTPRPP
                     AMMMALHIWSMSHGVASLFGRGDAARRKLPMSPEDLLEAQVLIYLRGLGFLTDRPAGQ
                     KDATDQKPAGPPPIPPSGPPSGPWGTPK"
     gene            3554..4036
                     /locus_tag="VGA15_04670"
     CDS             3554..4036
                     /locus_tag="VGA15_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HEX9587019.1"
                     /translation="MAYTADVNRWRGPDNEPYRPHMLDSPWHPGWIAVTVLGFIIWWP
                     IGLGLLLFTLGSRKMGCWNHQDRWANKMERMQYKMERMRGHMERRGFGFGGPPSSGNR
                     AFDEYRMETLRRLEEEQTEFKDFLDRLRHAKDKEEFDQFMAQHRTRPTPPPSDGQPQA
                     "
     gene            4445..5158
                     /gene="tolQ"
                     /locus_tag="VGA15_04675"
     CDS             4445..5158
                     /gene="tolQ"
                     /locus_tag="VGA15_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX9587020.1"
                     /translation="MNPADVAQSALPLASTDVSLIALFLQAHWIVKAVMLGLLACSVW
                     VWAIAIDKTFLYARTKRAMDRFEQAFWSGQSIEELYRALSAKPTQSMAACFVAAMREW
                     KRSFESQARSFAGLQMRIEKVMNVSIAREIERLERRLLVLATVGSAGPFVGLFGTVWG
                     IMSSFQSIAASKNTSLAVVAPGIAEALFATAIGLIAAIPATIFYNKFTSEVNRQAQRL
                     EGFADEFSAILSRQIDERA"
     gene            5198..>5362
                     /locus_tag="VGA15_04680"
     CDS             5198..>5362
                     /locus_tag="VGA15_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX9587021.1"
                     /translation="MAMNMAGSPGGGRRGRRRAVMAEINVTPMVDVMLVLLIIFMVSA
                     PLLTVGVPLDL"
BASE COUNT         1002 a         1616 c         1728 g         1016 t
ORIGIN      
        1 caaaggcggc aggagtttcc gcaatacgga gttttttacg ttcgaaggcg acaaggtcaa
       61 acgcatcgac gtgtatttcg gcgcgaccta cgagaatggc gcgttcgtga aacagcagca
      121 acaggaacag cagcaataaa agtgtgatcg acatgtcggt tcggcgcgat ctggttcgtc
      181 atcccgtgga atcattgtcg gatagctcca agcgaggaaa gagatgatga acacgaccag
      241 cgccgccgga ttgtcgaaga cgcgcctacg acgcatgcac gacgtcatgg ccggccatgt
      301 cgatgacggc agcatgcccg gcctggtgtc gctggtcagt cggcgcggcg aggtttttgt
      361 cgacgcgatc ggccgtatgg cgatcggcgg ggcgccgatg gcgcgcgaca ccatcttccg
      421 gatcacctcg atgaccaagc cgattaccgc cgtcgccgcg atgatcctgg ttgaggaatg
      481 caaattacgg ctcgatgatc ccgtcgaccg gtggctgccg gaactggcga accgcaaggt
      541 gctgcgcgcg atcgatgcgc cgctcgacga caccgtgccg gccaggcgcg cgatcacgct
      601 gcgcgatctg ctcacgttcc ggctcggtta cggcatgatc atggtgtttc cggatcgtta
      661 tccgattcag aaagccattg ccgaggcggg cttcgcgccg gggccggtgt ttccgtcctt
      721 cccgccggat gaactgatga agcgctacgg cagcctgccg ctgctttatc agcccggcga
      781 gcgctggctc tacaacagcg gaacggaaat tctgggcgtg ctgatcgcgc gtgtcgccgg
      841 caccacgttc ggcgagttct tatccgatcg catcttcgcg ccattgggaa tgaacgacac
      901 ggcgttcagc gtgccggaag ccaaacgcaa tcggtttgca acctgctatg tcggcgatca
      961 tgcgacgcaa agcccgaaag tcttcgacga tcccgtcgcc ggaaaattct caagcccgcc
     1021 tgtgtttgaa aacggcagcg ccgggctggt ctcgacggcc gatgatttca atgccttcgc
     1081 ccagatgatg ctgaacggcg gccgcatcgg gagcgaacgc attctgtcgc ggcgatcggt
     1141 cgaactgatg acgacggacc agatcacctc ggaacagaaa ttgggctcag aactgttttt
     1201 cggcgagaac cggggctggg gaatgggcct ctcggtgttc accaggcgcg acgatctcta
     1261 taccgcgccg ggtcgattcg gctgggacgg cggctatggc acctcttggt attccgaccc
     1321 gcgcgagcaa ctcaccggcc ttctgctgac ccagcgaatg atggattcgc cgcaaccgcc
     1381 gcgcgccttc gtcgatttct ggacctcggt ctatcaggcc atcgacgatt gattgcgcgg
     1441 caacggccct gcggttccgc cacaattacc tcggaaataa tttcgcctga catgtcggcg
     1501 ctgcaaaacc gggttcgtca tgttgacgag ccatggttcg gcgaggcgaa ccggccggtt
     1561 gatcaacgga gagtgacaat gcgattcatg gtgattgtga aagccgacag gaacagcgaa
     1621 gccggcgtga tgccgagcac ggaattgctg acggccatgg gcaagttcaa cgaagagatg
     1681 gtgaaggccg gcgtcatgct cgcaggcgag gggctgcacc cgagctcgaa gggcgcgcgc
     1741 attaaatttc caggcaaggg acgcaccgtg agtcgcggtc cgtttggcct taccggcgat
     1801 ctggttgccg gtttctggct gatccaggtc aaatcgctgg acgaggcgat cgaatggatg
     1861 aagcgagcgc cgttcgatga cggctccgag atcgaaatcc ggcaggtctt cgacgccgag
     1921 gactttggcg aggcgctgac gcccgaattg cgcgagcagg agcaacgtct ccgcgcgcag
     1981 gcagggaaga agtagaacca tccactgtca ttccgggatg cgcccactga cctcgggttt
     2041 acccgagttc agcaatatca ttgtccaagt cggcaacagc cgacttggat ggcgcaggcc
     2101 cggaatccat actcccggtc gtggttatgg attccgggct cgctcgcttc gctcgcgccc
     2161 cggaatgacg atgtgttgaa attcacctct atcaactgcc aaggacacca ccatgcgatt
     2221 catgatgctg atgattcccc tgggctacga gaccgcgccg cccgacgtca aactcgatcc
     2281 cgagcgcgtg gccgcgatga tgaaatacaa tcaggccctg aaagacgccg gcgtgctgat
     2341 cacgctcgac ggcctgcacc cgccgtcgat gggcgcgcgg gtgtcgttcg ccggcggcaa
     2401 gcctgtcgtg accgacggtc ccttcaccga ggccaaggaa gtgctcggcg gctactggat
     2461 gatcgacgtg aagtcgcggg aggaggcgat cggctgggcg aagcgctgcc ccgctaccgg
     2521 caacgagatc atcgaaatcc gccaggtaca ggaactctcg gattttcccg ccgacgttca
     2581 gcaagcggcg gccggactga gcgagttgca gggcaagccg gtacgatagt taacggccgg
     2641 tcaatacctc gtggcacagt tggcgtgcta gtgaccttcg cgccaggttc atgtaaaagc
     2701 ccttcacatg agctggcaca aggaacaacg ccgcgccgag cgcggctatc atcacggcaa
     2761 tctgaaagag gcgctgctgc aggcggcgct cggcctgatt gcggaaaaag gcgcggcggg
     2821 tttcaccttt gccgatgccg cgcgcatggc gggcgtcagc ccggcagcgc cgtaccggca
     2881 tttccgcgat cgcgacgagc tgctttcgag catcgcgcag cgcggtttcg agcagttcga
     2941 ggtggtgctg accaacgcct gggacgatgg ccggccggac accgtcacgg cgttcgagcg
     3001 ggtcggcaag gcctatctcg cgttcgcccg cgacgagccg gcgttctatt cggcgatgtt
     3061 cgaatcgggg cttcccgtcg accttaatcc aaccttgttg gcggcgagcg agcgcgcttt
     3121 cggcattatc cgcgccgccg ccgagcgcct cgccgcgatg gcgccgcccg gcacgccgcg
     3181 cccgccggcg atgatgatgg cgctgcatat ctggtcgatg tcgcacggcg tggcgtcgct
     3241 gttcggacgc ggcgatgcgg cgcgccgcaa gctgccgatg tcgccggagg atctcttgga
     3301 ggcgcaggtg ctgatctacc tgcgtggcct cggtttcctg accgaccgtc ccgctgggca
     3361 aaaagacgcg acggatcaaa agccggccgg gccgccgccg ataccacctt cgggtccgcc
     3421 atcaggtcct tggggcaccc caaaataaat tcgagcgcgc cgctggcgcc cggcttgaca
     3481 aaaatccggg atgggttagg tatgtaaatg ttatttacat tcacaacggc gtgatgccgt
     3541 catggagagg gaaatggcct acaccgcaga tgtcaatcga tggcgcggcc ccgataacga
     3601 gccttaccgc ccgcacatgc ttgattcgcc ctggcacccc ggctggatcg ccgtgaccgt
     3661 gctcggtttc atcatctggt ggccgatcgg gcttggcctt ctccttttca cactcgggag
     3721 cagaaaaatg ggttgctgga atcatcaaga ccgctgggcc aacaagatgg agcggatgca
     3781 gtacaagatg gagcggatgc gcggccacat ggagcgccgc ggcttcggct tcggcggccc
     3841 gccctcgagc ggcaaccgcg cgttcgatga gtaccgcatg gaaaccctgc ggcgcctcga
     3901 ggaagagcag accgagttca aggatttcct cgatcggctg cgccacgcca aggacaagga
     3961 agagttcgac cagttcatgg cgcagcatcg cacccggccg acgccgccgc cctcggacgg
     4021 tcagccccag gcgtaagtct ttcgaaatcc ggaaggctca gcctcgggcg cattgccacg
     4081 gtcgaatctg aggcgaaaaa gccgcccgtt tgcgataagc agacgggcgg ttttgcttgt
     4141 ttgggagccc gattttggag cagcccgtcg atcccccgct gccgcccttt gccgcagcca
     4201 aattcccctt tggtaacctc gcattaacca tgattggcgt ctggttaggg gcgtaacgtc
     4261 gcgctgatag ccctcgtttt gacatgttgg cggcggatgc aggcctcggc ttcggcgggc
     4321 ctcccatccg acacaacaag gcgtctgcgt tggcgctcac cgccgcgccc gacgcgcggc
     4381 tgtggaaccg gcagccattt gctcccggag gatttgggaa gcttgcgttg agaggatacc
     4441 gcttatgaat ccggccgacg tggcccagtc agcgttgccg ctggcatcca ccgacgtatc
     4501 gctgatcgca ctgttcctgc aggctcactg gatcgtgaaa gcggtcatgc tggggctgct
     4561 ggcctgttcg gtctgggtgt gggcgatcgc gatcgacaag acgttccttt atgcgcgcac
     4621 caagcgcgcg atggatcgct tcgaacaggc gttctggtcc gggcagtcga tcgaggagct
     4681 ttatcgcgcg ctctccgcca agccgacgca gtcgatggcg gcctgcttcg tcgcggcgat
     4741 gcgcgaatgg aaacggtcat tcgaaagcca ggcgcgatcc ttcgccgggc ttcagatgcg
     4801 gatcgagaag gtcatgaacg tctcgatcgc ccgggaaatc gagcggctgg aacgccgcct
     4861 gctggtgctg gcgacggtcg gctcggcggg cccgtttgtc ggcctgttcg gcacggtttg
     4921 gggcatcatg tcgagcttcc agtcgatcgc cgcctccaag aacacttccc tggcggtggt
     4981 ggccccaggc atcgcggagg cgctgttcgc aaccgcaatc ggccttatcg ccgccattcc
     5041 ggcgactatt ttctacaata agttcacctc cgaggtgaac cggcaggctc agcggctgga
     5101 agggttcgcc gatgaatttt cagcgatcct gtcgcgccag atcgacgaac gggcgtgagg
     5161 cgcagtatgc tatgaagcgc atcgtgagaa ccatatcatg gcgatgaaca tggcaggctc
     5221 gccgggcggc ggacgccgcg gccggcgtcg cgcggtgatg gcggagatca acgttacgcc
     5281 gatggtggac gtaatgctgg tgttgctgat catcttcatg gtgtcggcgc cgctgttgac
     5341 ggtcggcgtg ccgctcgatc tg
//
LOCUS       DASWGM010000154        11034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2644983,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000154 DASWGM010000000
VERSION     DASWGM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11034
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2644983"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VGA15_04685"
     CDS             complement(<1..339)
                     /locus_tag="VGA15_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX9587022.1"
                     /translation="MQTTLLGLAIAFIIALIAALIGPYFIDWNQFRPQFETEATRVVG
                     APVRVGGALDARLLPTPSLRLRSVTVGGANDLGKVRADKLDVEFSLGSLMRGEWRATE
                     LTINGMALDLG"
     gene            complement(552..1784)
                     /locus_tag="VGA15_04690"
     CDS             complement(552..1784)
                     /locus_tag="VGA15_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9587023.1"
                     /translation="MNEHVQTATTEPLFDPLAPEFIRDPYPFYERLRATDPMHLTPFG
                     TFAASRHAEVSLVLRDKRFGKDYVERTVRRYGPQIMEEPVFRSMSHWMLQQDPPDHTR
                     LRGLVVKAFTARRVEDMRPRIQRIVDETLDAIAGQGHMDLIEDFAFRLPVTIICDMLG
                     IPEDHREVFYKSSRNGGRLLDPVPLSPAEIAEANAGNIMAKAYFQQLFELRRRNPGDD
                     LTTQLVHAEEDGSKLSNEELTANIILLFGAGHETTVNLIGNGLLALHRNPDQLALLKA
                     NPALITNAIEEFLRYDSSVQLTGRVTLEDIDELGGKKIPKGESVLCLLGSANRDPAVY
                     PDRPESLDITRPNVRPLSFGGGIHFCLGAQLARIEAEVAIATLLRRLPDLRLDDAVNP
                     EWRPTFVLRGLKQLPASW"
     gene            complement(1962..3275)
                     /locus_tag="VGA15_04695"
     CDS             complement(1962..3275)
                     /locus_tag="VGA15_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587024.1"
                     /translation="MRLPFFYGWIVVAVTFVTMAIGVNARTSFSLLFPPIIDEFGWER
                     GVTAGAFSFGFLASGVASPLIGRMMDRFGPRAVMELGIALMGGGLLLAPLTTQPWHLY
                     LTLGVMVGAGSVCLGYSGQSLFLPNWFNRRRGLALGLAFTGVGIGSITLLPWAQHMIE
                     QTGWRTACTAIGILVLVVLAPINLLLRKRPEDIGLRSDGDAAPSPSAKPVSYIVDPDW
                     AGIDWTLSRAVRTARFWWLALGYFCALYIWYAVQVHQTKYLLDIGFSPGVAVWALGVV
                     SLLGIPGQIVLGHLSDRLGREWIWTASCLGFAICYAALIALAHVPTLMLVYVVVLAQG
                     ALGYGLTSILGAVVVEIFQGKHYGSIFGSVMLAGLAGGASGPWITGLLHDLSGNYTIA
                     FAIGIGVSGLSAVAIWMASPRKIRTVAGQLHRARDAASMASVYRG"
     gene            complement(3469..4575)
                     /locus_tag="VGA15_04700"
     CDS             complement(3469..4575)
                     /locus_tag="VGA15_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX9587025.1"
                     /translation="MRITDVKAHHLRIPFDAGVASFRQGVSAIAALDMVIVEVLTDTG
                     LTGWGDAFAYVCPRTTMTAIEEMIAPQARGLEVPDAEGIPDFMDRIQRNLHLFGRYGI
                     TMFAISGLDIALWDLAARVKGVPLHRLLGASKRSRIPAYASLLRIGTAESVASECQTA
                     LRRGYTAIKLHETTTPAVFAARQATGAGVPLMVDMNCPLDGAEAIAFAQACRAATPMF
                     LEEPVWPPEDFATLAEVRAKGGLAIAAGENACTVHQFRQMMAAGAVSYAQPSVIKVGG
                     ITEYLKVAALADEMGIRLAPHSPYFGPGLLATLQLMSLRDDGSFIEIFYMNRAACLWH
                     GQIDADANGSIEVPEGPGLGYEPDKEIMERYRVS"
     gene            complement(4644..5636)
                     /locus_tag="VGA15_04705"
     CDS             complement(4644..5636)
                     /locus_tag="VGA15_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX9587026.1"
                     /translation="MKVLLTHTPQARRQYYGERSLNGLQAVAQVVLHDADDALDAAGL
                     IEAARDVDIIVADRLTEGPAEIFADLPKLRAFVRCAVDIRNIDVEAASAAGVLVTRAG
                     PGFVPSVAELALGFMVDLSRGMSRATADYHSGRKPEIVMGRQLAGNCLGIIGYGSIGQ
                     YLARIAKALGMEVLVADPFATVSDADIKHLSLEDLLERSDYVVCLAVANESTENLIGQ
                     AALAQMRPHAFFINLSRGNLVDEAALTAALRENRIAGAAMDVGRALDQMPTPELAKLP
                     NVIATPHIGGLTPPAIESQSFETVRQVEAVIDGKVPAGAVNAAHWTRRPSAKTD"
     gene            complement(5667..7520)
                     /locus_tag="VGA15_04710"
     CDS             complement(5667..7520)
                     /locus_tag="VGA15_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9587027.1"
                     /translation="MSGAAPLQSGGHRIIASLLVLSDATAAILLAADLLVVCGSVLLR
                     FLFNAPVEWADDVARGLMVGSSFFGAASALARSENLGVAFFIDKLPASVRHVVDSFGA
                     LLVMVIAGYVAFNALKLGWLTTGQTSGSGLPLELTFYPMGAGALFMTIFAFEVFCSRP
                     LRVMIAGAVAAGLIACLYLAWDFLGPASVPSPGALMLVGFFVTLFGGLPIGFALALAA
                     LIFIWVEGSLPGVIFAQQMARGIDNFVLLAIPFFILVGYLMEANGMSVRLIELLQRAV
                     GRMRGGLNVVMVTSMVLFSGISGSKMADVAAVGSVLIPAARRSRQNPGSAVALLAASA
                     VMAETIPPCINLIILGFVANLSIGGLFVAGLLPAGLMALALIVVSIMFGKRSASAEEI
                     EPQIPMSGLWSGAIASFGLIFMIFAGFKSGFATATEISAFAAMYAIVVGRLVFGELGF
                     KTAAHSFVQAATRSGLVLFIVAAAQSLAFILTLQQVPHTVGEMMLSLSAAHGTWLFML
                     LSIAVLIVMGSVLEGAAALIIFGPLLLPVAVKLGIDPLHFGVVLVISMGLGLFAPPLG
                     LGLYGACLIGNVPIEQTVKPIMGYLGLLLLCLLVIAFIPGISTTLPRVFGY"
     gene            complement(7532..8563)
                     /locus_tag="VGA15_04715"
     CDS             complement(7532..8563)
                     /locus_tag="VGA15_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9587028.1"
                     /translation="MKTVSVSRRAFLKSSKATAVWLALSPAIVGRAQAATTKLKCSSS
                     LPNDPKYANGRVYYDNLVRNLKANGLGEQIEVAFFPDNQLGQEIDVINSVKLGVIDLM
                     VSGSSISANLVPLVGTFDLGYLFSSFPQQTKAFDAGAAKPIEEALLKGANIRIISWAY
                     NFGSRSVLAKKPVKTPEDLAGLKIRTLPNPVITECLRLMGAAATPLAFGEIYTALQAG
                     VLDGLEHDPPTILASKFYETSKHYALTQHNFSPLATYFSDATFNRMDPKLREGFLDAA
                     QKAAADTRTHGLEMEKEALKNLADKGVTIVECDKEAFRKRVLPQSDNFVKARPEAKPV
                     VDMIRATQA"
     gene            complement(8687..9433)
                     /locus_tag="VGA15_04720"
     CDS             complement(8687..9433)
                     /locus_tag="VGA15_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587029.1"
                     /translation="MLTSAAESLLAVDRLESGYGKIRVLHGIDFTVSPGEVVALLGPN
                     GAGKTTMLRALSGLLPVNAGHVRFDGRDMTNATPREAARAGLVHVIEGHRVFTQISVT
                     DNLLLAGYDLPRGERAARVEEALSFFPEIAEKRHERGGALSGGQQQMLTVAQGLVRRP
                     RLLMLDEPSAGLSPVLVDRVLNVIGQLRRQGTAVLLVEQLLEKALAAADRVYALVQGH
                     IVLEAPTSEANLPQRLERAYFGHEAKVDVA"
     gene            complement(9414..>11034)
                     /locus_tag="VGA15_04725"
     CDS             complement(9414..>11034)
                     /locus_tag="VGA15_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9587030.1"
                     /translation="LNLVVGFAGHLAIGYVALLTLGAYTTSVLVAGNVMPPVPVFAAL
                     PIAACVGAVFGVIVGLPALRLRTFYFAMSTLGFATIVTQIALAWQSVTGGGIGISGPE
                     FPAPFNTAWGFYYLCIACAAVSTWISANVAHSRFGRALIAVRDAEVAAEATGISKQRM
                     LIAIFLLAGALAAIAGGLFATLQTYITPDAFTFDLSILFFIAILIGGRGSILGPMLGT
                     IILTILPEIAAPLAAWSTFLYAVLLLVIVLVMPGGIAALLDFRNRRPLASNRAIIPRP
                     AALGSVVRASAGGRKLSLRGIALSFGNVRAIDGLDLDVAPGQIHGLIGPNGSGKTTTL
                     NVISGYYAARAGTMQLGEDVLPPGMPVLRAARGIGRTFQTPRVIGEASVLQNVMIGGT
                     IEGRATFVEALLALPRNQRDERMLSARARALLGVVGLEMLADVRADRLQHSELRFIEI
                     ARALMLEPDFLLLDEPAAGLSGDEIERLAALIKAISRRGAGILLVEHHADLIFDICDE
                     VTVLNLGRTLAAGTPAEIRVHKEVVSAYLGG"
BASE COUNT         2297 a         3507 c         3404 g         1826 t
ORIGIN      
        1 accaagatcg agcgccatgc cgttgatcgt cagttcggtg gcgcgccatt cgccgcgcat
       61 cagcgagccc aggctgaact cgacgtcaag cttgtcggcg cgaaccttgc cgaggtcgtt
      121 ggcgccgccg acggtcaccg aacgcaaccg cagcgacggc gtcggcaaca ggcgcgcatc
      181 caacgcgccg ccgacgcgca ccggcgcgcc gaccacccgc gtagcctccg tctcgaattg
      241 cggccggaat tgattccagt cgatgaaata cggcccgatc agtgcggcga tcagcgcgat
      301 gataaaggcg atcgccagtc cgagcagcgt cgtctgcacg gtgtctcctc ttgagcaggt
      361 tccggcgcgc tggccttgcg cggcgtatga cgcggtttat ccgccaggtt gcacctgcca
      421 tcgcgacggt aacccgaagc cgccgggccg ccaccgcgct ctgcaatcag cgccggaaca
      481 atgccctata tagagtcatc ggtggcgaag tcacaacaac ctcgtgtgac ggcttcacgc
      541 aagaggcgcc atcaccagct cgcgggcagc tgcttgaggc cgcgcaatac aaaggtcggc
      601 cgccattcag ggttaacggc gtcgtccagc cgcagatccg gcaggcgacg caacagggtc
      661 gcgatcgcga cctctgcctc gatacgcgcc aactgggcgc cgaggcagaa atggatgccc
      721 ccgccaaacg acagcggacg cacattggga cgggtgatat cgagactttc cggccggtcg
      781 ggatagaccg ccgggtcgcg attggccgaa cccaagaggc agagcacgct ttcacccttc
      841 gggattttct tgccgccgag ctcgtcgatg tcttccagcg tgactcggcc ggtcagctgc
      901 accgaggagt cgtagcgcag aaactcttcg atagcgttgg tgatgagggc gggattggcc
      961 ttcagcagcg cgagctgatc gggattacga tgcaacgcca gcagaccgtt accgatcagg
     1021 ttgacggtgg tctcatgccc ggcgccgaac agcagaatga tattggcggt gagctcctca
     1081 ttggagagct tgctgccgtc ttcttcggca tgcaccaatt gcgtcgtcag gtcgtcaccg
     1141 ggattgcgcc gcctgagctc gaacagctgc tggaaatagg ccttggccat gatgttgccg
     1201 gcgttggctt cggcgatttc ggccggcgac agcggcaccg gatcgagcag gcgtccacca
     1261 ttgcgcgaac tcttgtagaa aacctcgcgg tggtcctcgg gaattccgag catgtcacag
     1321 atgatcgtca ccggcagccg aaacgcaaaa tcctcgatca ggtccatgtg gccctgcccg
     1381 gcgatggcat cgagcgtttc atcgacaatg cgctggatgc gcggacgcat gtcctcgacc
     1441 cggcgcgccg tgaacgcttt caccaccaga ccgcgcaggc gggtgtggtc gggcgggtcc
     1501 tgctgcagca tccagtggct catgctgcgg aagaccggct cctccatgat ctgaggtccg
     1561 tagcgtcgca cggtgcgttc gacataatcc ttgccgaaac gcttgtcgcg cagaacgaga
     1621 ctgacctcgg cgtgccggct cgccgcgaaa gtaccaaacg gcgtgagatg cattggatcg
     1681 gtggcgcgca gccgctcgta gaacggatag ggatcgcgaa taaactccgg cgccagcggg
     1741 tcgaacagcg gctcggttgt cgcggtctga acatgctcgt tcatgggcgc ctcgatgcgg
     1801 tcagatcaat ttggaaccgg attaacccac aagccaccgg tatcggcaag ccctccatga
     1861 tcaactcttc tcagggttgt actcaagccg cggcaacgcc gatagcagac aggcgcgtcg
     1921 acctgcgtct acgtcgccgc cgtccgctcc ggactatttc atcatccgcg atagacagag
     1981 gccatgctgg cagcgtcccg tgcccggtgc agctggcccg cgacggttcg aatcttgcga
     2041 ggtgacgcca tccagatcgc caccgccgac aatccgctca cgccgatacc gatcgcaaac
     2101 gcgatcgtgt agttccctga aagatcgtgc aggagcccgg tgatccaggg acccgaggca
     2161 cctccggcga ggcctgcgag catcacgcta ccgaaaatac tgccgtaatg tttgccctga
     2221 aatatctcga caaccacggc acccaggatg gaggtcagac cgtagccgag cgcgccttgc
     2281 gccagcacta cgacgtagac cagcatcaag gtgggaacgt gcgccagcgc gatcagtgcc
     2341 gcatagcaga tcgcaaagcc gaggcagctc gcggtccata tccattcgcg ccccagccgg
     2401 tcggagaggt gtccgagcac gatctggccg ggaataccga gcaggctgac cacgcccagt
     2461 gcccagaccg cgacgcccgg actaaagccg atgtccagaa ggtatttggt ctggtgaacc
     2521 tgcaccgcgt accagatata caatgcgcag aaatagccga gcgccagcca ccagaagcgc
     2581 gcggtgcgaa ccgcgcgact taaggtccag tcgatgccgg cccaatcggg atcgacgata
     2641 taggataccg gtttcgcgga tggcgaaggt gctgcgtctc catccgaccg aagcccgatg
     2701 tcctccggcc gctttcgcag caacaggttg atcggcgcaa gcacgaccag aaccagaata
     2761 cctatcgccg tgcaggccgt tcgccagccg gtttgctcga tcatgtgctg cgcccagggc
     2821 agcaaggtaa tggagccgat accgacgccg gtgaatgcca gtcccaaggc gaggccacgc
     2881 cggcgattga accagttggg cagaaacaac gattgacctg agtagccgag acagacgctt
     2941 ccagcgccga ccatgacacc caacgtcaga tacagatgcc acggctgcgt cgtcagcggc
     3001 gccagcaaca accctccccc catcaacgcg atgccgagct ccatgacggc gcgaggaccg
     3061 aagcgatcca tcatccggcc gatcaaggga ctcgcgacac cggaagccaa aaagccgaat
     3121 gagaaggcgc ccgccgttac gccgcgctcc cagccgaact cgtcgatgat gggtggaaac
     3181 agcagcgaaa acgacgtgcg ggcgttgacg ccgatcgcca tggtgacgaa cgtcacggcg
     3241 acgacgatcc agccgtaaaa aaagggaagc cgcattgggt tacgtacctt gtcatcctcc
     3301 ggtggccaat tgcacatccg aggtccggtc cttgggacca tcagggaatg acggggtcaa
     3361 gatttcttag tgcggaggta ttaaagatgt cgcggaatgg ctaaccgccg tcagcattca
     3421 tgtccgaaat agacgcgctc aagccgctgc acattgaaga caaatcggtc atgacacccg
     3481 gtaacgctcc atgatctcct tgtccggctc gtagcccagc cccggccctt cgggcacctc
     3541 gatgctgcca tttgcatcgg catcgatttg gccatgccaa agacaggcag cgcgattcat
     3601 gtagaaaatc tcgatgaacg agccgtcgtc acgcagcgac atcagctgta acgtcgccag
     3661 aaggccgggc ccgaaatacg gcgaatgcgg tgcaagcctt atacccatct cgtcggccaa
     3721 cgcagcgact ttcagatatt ccgtgatgcc gccgaccttg atcaccgaag gctgggcgta
     3781 actcacggcg cccgcggcca tcatttggcg aaattgatga accgtgcagg cgttctcccc
     3841 cgcggcgata gcgagcccgc cttttgcgcg cacctctgcc aaggtcgcga agtcctcggg
     3901 cggccacacc ggttcctcga gaaacatcgg cgtggccgct cgacaagcct gcgcgaatgc
     3961 gatcgcttcg gctccatcca atgggcaatt catatcgacc atgagcggga ccccggcgcc
     4021 ggtcgcctgc cgggctgcga atacggccgg cgtggtcgtc tcatgcagct tgatagccgt
     4081 gtagccacgt cgtagcgcgg tctggcattc gctcgcaaca ctctccgccg ttccgatccg
     4141 aagcaggctg gcataggcgg gaatccgcga ccgcttgctg gcgccgagca ggcgatgcaa
     4201 cggcacgcct ttcaccctgg cggcaagatc ccacaacgcg atgtccagtc cggagatcgc
     4261 aaacatcgta atgccatagc gaccgaacag atgcagattg cgctgaatgc gatccatgaa
     4321 gtcgggaatg ccttcggcat ccggcacttc gaggccacgg gcctgcggcg caatcatctc
     4381 ctcaatagct gtcatcgttg tccgcgggca gacgtatgcg aaagcatcgc cccaaccggt
     4441 gaggccggtg tcggttagga cctcgacaat aaccatatcg agcgccgcaa ttgccgaaac
     4501 gccttgcctg aaactcgcca cgcccgcatc gaaaggtata cggagatgat gtgccttgac
     4561 gtcggtgatc cgcatgcctg cctccctgtt taccgcaatt gccgatgcta tcggcgaatt
     4621 tatcaagagt tcagagcgcg ctatcaatcc gttttcgctg aaggacgacg cgtccaatga
     4681 gcggcattga cggcacctgc cggaaccttg ccatcgatga ctgcttcaac ttgacgaaca
     4741 gtttcgaatg actggctctc aatcgccggc ggggtcaacc caccgatatg cggcgtagct
     4801 atgacattag gcagcttcgc tagttccgga gtcggcatct gatccagggc gcggccgacg
     4861 tccatcgccg ctccggcaat gcggttctcg cgcaacgcag cggtcaatgc cgcctcatcg
     4921 acgagattgc cgcgggatag attgatgaag aaggcgtgcg gccgcatctg cgccagggct
     4981 gcctgcccga tcaaattttc cgtgctttca ttagcgactg ccaggcaaac aacatagtcg
     5041 gaacgctcaa gaaggtcttc gagcgacaga tgcttgatgt ccgcgtcact gacggtcgca
     5101 aagggatcgg cgaccagaac ctccatgccc agcgcttttg cgatccgggc aaggtattgg
     5161 ccgatgctgc cgtagccaat aatgccaaga cagttgcccg ccagctgccg gcccatcacg
     5221 atctccggtt tgcgccccga atggtaatcc gccgtggcgc gcgacattcc gcgcgacagg
     5281 tcgaccatga agccgagcgc cagttccgcc accgatggca cgaagccggg cccggcgcgc
     5341 gtgaccagca cgcccgccgc ggaggctgct tcgacatcga tgttgcggat gtcgaccgcg
     5401 caacgaacga atgcgcgaag ctttggcagg tcggcgaaaa tctcggccgg cccttccgtc
     5461 aaacgatccg ccacgatgat atcgacatcg cgcgccgcct cgatcaggcc ggcggcatcc
     5521 agcgcgtcgt cggcgtcgtg cagcacgacc tgcgcaaccg cttgcaggcc gttcaggctg
     5581 cgctcgccat aatactggcg ccgcgcctgt ggcgtatggg tcagcaggac ttttatcatc
     5641 gaacggtttc tcccggttcg cggcggtcag tagccaaaga cgcgcggcag cgtagtactg
     5701 atgccgggaa taaaggcaat caccagcagg cacagcaaca gcaatccgag atagcccatg
     5761 atcggcttga cggtttgctc gatcggcacg ttgccgatca ggcaggcccc gtagagtccg
     5821 agaccgagcg gtggcgcgaa caacccgagc cccatcgaga tcacaagtac cacgccgaaa
     5881 tgcaagggat cgatgccgag cttgaccgcg accggcagca ataacggtcc gaagatgatg
     5941 agcgccgcgg cgccctcgag caccgagccc atcacgatca gcaccgcgat cgacagcagc
     6001 atgaacagcc aggtgccgtg cgcggccgac agcgacaaca tcatctcgcc gaccgtatgc
     6061 ggcacttgct gcaaggtgag gatgaaggcg agtgattgcg cggccgccac gatgaacagc
     6121 accaatccgg agcgcgtggc cgcctgcacg aagctgtgtg ccgcggtctt gaagccgagc
     6181 tcaccgaaca caagtcggcc taccacaatt gcatacatcg cggcgaacgc cgaaatttca
     6241 gtggcagtgg cgaacccgct cttgaagcct gcgaagatca tgaagataag tccgaacgat
     6301 gcgatcgcac cgctccaaag gcccgacatt ggaatctgcg gttcgatctc ctcagcggat
     6361 gccgagcgct ttccaaacat gatcgacacc acgatgagcg ccaatgccat caaccccgcc
     6421 ggcagcagcc cggcgacgaa caggccgccg atcgacaggt tggcgacaaa gccgagaatg
     6481 atcagattaa tgcaaggcgg aatggtttcc gccatgacgg ccgacgccgc cagcagggcc
     6541 accgcgctac ccggattctg ccgggaacgg cgggcggcag ggatcaacac cgatccgacc
     6601 gccgcgacgt cggccatctt ggagccggag atgcccgaaa acagcaccat cgacgtcacc
     6661 atgacgacgt tgaggccgcc gcgcatccgc cccaccgcgc gctgcagcag ttcgatcagg
     6721 cgaaccgaca tcccgttggc ttccatgaga tagccgacca gaatgaaaaa cgggatcgca
     6781 agcagcacga aattgtcgat gccacgcgcc atctgctgcg cgaagatgac gccgggcagc
     6841 gagccttcga cccagatgaa aatgagcgcc gccagcgcca gcgcaaagcc gatcggcaat
     6901 cccccgaaca aggtgacgaa aaagccaacc agcatcagcg cgcccggcga cggcaccgac
     6961 gccgggccta agaaatccca cgcgagatag aggcaggcga tcaggccggc ggcgaccgct
     7021 ccggcgatca tcacgcgcaa cggacgcgag caaaaaacct cgaacgcaaa gatcgtcatg
     7081 aacagcgcgc cggcgcccat tggatagaac gtcagttcca gcggcaggcc ggatccggag
     7141 gtttgcccgg tggtcagcca gccgagtttt agggcgttga acgccacata gcccgcgatc
     7201 accatcacca gcagcgcgcc gaaggaatca acgacgtgcc ggaccgaagc cggcagcttg
     7261 tcgatgaaaa atgccacgcc gagattctcg ctgcgggcca gcgcgctggc ggcgccaaag
     7321 aagctcgacc cgaccatgag cccgcgggcc acatcgtcgg cccactcgac cggcgcgttg
     7381 aacaggaagc gcagcaacac cgaaccgcag acgaccagga gatcggctgc gaggaggatc
     7441 gccgccgtcg cgtcacttaa tacaagcagc gaggcaatga tccggtggcc gccgctttgc
     7501 aaaggcgcgg cgcctgacat ggtgtgtacc ctcaagcctg tgtggcgcgg atcatgtcca
     7561 ccacgggttt cgcttcgggg cgagccttga cgaaattgtc ggactgaggc agcacgcgct
     7621 tgcgaaatgc ttccttgtca cattcgacaa tcgtgacgcc cttgtccgcg agattcttga
     7681 gcgcctcctt ctccatctca agaccatgag tgcgggtatc ggctgccgcc ttttgggctg
     7741 cgtcgagaaa tccttcacgc agtttcgggt ccatccgatt gaacgtcgca tcgctgaaat
     7801 aggtcgcgag cggcgagaaa ttatgctgcg tcaacgcgta gtgcttggaa gtctcgtaga
     7861 atttgctggc gaggatggtc ggcggatcat gttcgagccc gtcgagcacg ccggcctgca
     7921 aggccgtgta gatttcaccg aacgccagcg gcgttgccgc agcacccatc aggcgaagac
     7981 actcggtgat gacgggattg ggcagggttc ggatcttgag gcctgcgaga tcttccggcg
     8041 tcttcacggg cttcttggcc agcacgctgc gcgaaccgaa attgtaggcc caggagatga
     8101 tgcggatatt cgcacccttg agcagagcct cctcgatcgg cttggcggcg ccggcatcga
     8161 atgcttttgt ctgctgtgga aaactggaga acagataacc gaggtcaaag gtcccgacca
     8221 gcggcaccag attggccgag atcgaagagc ccgacaccat caggtcgatc acgcccagtt
     8281 tgacggaatt gatcacgtcg atttcctgac cgagctgatt gtcggggaag aaagcaacct
     8341 cgatctgctc gccaagtccg ttggccttca gattcctgac caaattgtcg tagtagacgc
     8401 ggccgttggc gtatttggga tcgttgggaa gtgatgatga gcatttgagt ttcgtcgtgg
     8461 cggcctgcgc gcgcccgaca atagctggcg acaaggcgag ccacacagct gttgccttcg
     8521 acgatttcag aaacgcgcga cggctgacgg acacagtctt catatcgttt cctccacgcg
     8581 cgttttgtca cgccgctatt ggttcgcggc cgtgcgctta tggttgaccg cgaatgtatg
     8641 accaaacgga agcctgttgc aaggcaggac cgcaaatgtc tggcgatcac gcgacatcca
     8701 ccttcgcttc gtggccgaaa tacgcgcgct cgagccgctg cggcaggttg gcttcgctgg
     8761 tcggcgcttc cagcacgatg tgcccctgca ccagtgcgta gacgcggtcg gcggcggcga
     8821 gcgccttttc caggagctgc tcgaccagca gcaccgcggt gccttgcctg cgcaactggc
     8881 cgatgacgtt gagcacgcga tcgaccagca ccggcgacag accggccgac ggttcgtcga
     8941 gcatcaacag gcgtggccgg cgcaccagcc cctgcgccac cgtcaacatt tgctgttggc
     9001 cgcctgagag cgcgccgccg cgctcgtggc gcttctcggc gatctcggga aagaacgata
     9061 gcgcttcctc gacccgcgcc gcgcgctcgc cgcgcggcag gtcgtagccc gccagcagga
     9121 gattgtcggt aacggaaatc tgcgtgaaca cccggtggcc ttcgatcaca tggacaagcc
     9181 cggcgcgcgc cgcctcgcgc ggcgtggcgt tggtcatgtc gcggccatca aagcgcacat
     9241 gtccggcatt gaccggcagc aatcccgaca aggcacgcag catggtggtc ttgccggcac
     9301 cgttgggacc gagcagcgcc accacctcgc ccggcgacac cgtgaaatcg atgccatgca
     9361 gcacgcggat cttgccgtag ccggactcca gccggtcgac ggcgagcagc gattcagccg
     9421 ccgaggtaag cactgaccac ctccttgtga acccgaatct ccgcaggcgt tccggcggcc
     9481 agcgtgcggc cgagattgag cacggtgacc tcatcgcaaa tgtcgaagat caggtcggca
     9541 tggtgctcga ccagcaggat gccggcaccg cgacggctga tcgccttgat cagcgcggcc
     9601 aggcgctcga tctcgtcgcc ggatagtccg gcggcgggct cgtcgagcag caggaaatcg
     9661 ggctccagca tcagcgcccg cgcgatctcg atgaaccgca gttcgctgtg ctgcaggcga
     9721 tcggcgcgga cgtcggccag catttcaagt ccgacgacgc cgagcagcgc gcgggccctg
     9781 gcgctgagca tgcgctcgtc gcgctggttt cgcggcagcg ccagcagcgc ttcgacgaat
     9841 gtcgcccgtc cctcgatggt gccgccgatc atcacgttct gcagcaccga ggcctctccg
     9901 atcacgcgcg gcgtctggaa ggtgcgaccg atgcctctgg cggcgcgtag caccggcatg
     9961 ccgggcggca gcacatcttc tcccaactgc atggtcccgg ccctggcagc gtagtaaccg
    10021 gagatcacgt tcagggtcgt ggtcttgccg ctgccattgg ggccgatcag gccgtgaatc
    10081 tggccgggcg cgacatcgag atcgaggccg tcgatggcgc gcacattgcc gaaactcagc
    10141 gcgatgccac gcagactgag tttcctgccg cccgcgctcg cgcgcacgac agagccgagc
    10201 gccgcgggcc gtggaatgat ggcgcggttg ctggcaagcg gccggcggtt gcgaaaatcc
    10261 agcagtgcgg cgatgccgcc gggcatcacc agcacgatca ccagcaacaa taccgcgtag
    10321 aggaaagtcg accacgccgc gagcggtgcg gcgatctccg gcaggatggt caggatgatg
    10381 gtcccgagca tcgggccaag gatcgagccg cgtccgccga tcaggatcgc aatgaagaac
    10441 aggatcgaca gatcgaaagt gaaggcgtcc ggcgtgatgt aggtctgcaa ggtagcgaac
    10501 aatccgccgg cgatcgcggc cagcgcgccg gcgagcagaa aaatcgcgat cagcatgcgc
    10561 tgcttggaaa tgccggtggc ttccgccgcg acttcggcgt cgcggaccgc gatcaaggcg
    10621 cggccaaaac ggctgtgagc gacgttagcg ctgatccagg tcgagacggc ggcgcaagcg
    10681 atgcagaggt agtaaaagcc ccacgcggta ttgaacggcg ccggaaattc cggacctgat
    10741 atgccgatgc cgccgccagt gacgctctgc caggccagcg cgatctgggt gacgatggtg
    10801 gcgaaaccga gcgtcgacat tgcgaaatag aaggtgcgca accgcagcgc cggcaggccg
    10861 acgatgacgc cgaacacggc gccgacgcaa gcagcgatcg gcaatgccgc gaagaccggc
    10921 accggcggca tgacattgcc ggcgaccagc acgctggtgg tgtaggcgcc gagcgtcagc
    10981 agcgcgacat agccgatggc cagatgtccg gcgaagccga ccacaaggtt gagg
//
LOCUS       DASWGM010000155         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_148618,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000155 DASWGM010000000
VERSION     DASWGM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148618"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="zapE"
                     /locus_tag="VGA15_04730"
     CDS             complement(<1..842)
                     /gene="zapE"
                     /locus_tag="VGA15_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX9587031.1"
                     /translation="MLSTPSASFSAHYQALVASGVIEPDAAQAEAADAFADLERRLAG
                     YKPLRKQGLLGRLFADKDGAPPRGLYVHGDVGRGKTMLMDLFFQHSQVEHKRRAHFHE
                     FMAEVHERIYGYRQNIARGEIADGDVIALTAGSIFDEAWLLCFDEFHVTDIADAMILG
                     RLFSKLFELGTVVVATSNVAPEDLYKGGLNRALFLPFIAQISDHMDVLRLDARTDFRL
                     EKLAGVKMWLVPADRAADAALDQAWARMTGKAPCKPRDISIKGRVLHVPCSADGVARF
                     SFAE"
     gene            complement(1239..>1575)
                     /gene="thpR"
                     /locus_tag="VGA15_04735"
     CDS             complement(1239..>1575)
                     /gene="thpR"
                     /locus_tag="VGA15_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX9587032.1"
                     /translation="VALQGLSSFGGRKPRAVVASIAPSRPLIELQAELERLMQRFGLD
                     PEGRKFTPHVTLARLRDASNQDVADYLSVRGYFPTRVFTASRFVLFSSRASTGGGPYV
                     VEDSYALSE"
BASE COUNT          329 a          500 c          480 g          266 t
ORIGIN      
        1 atctccgcga acgaaaatcg cgcaacgcca tccgccgaac acggcacatg cagaacacgg
       61 cccttgatcg agatatcgcg cggcttgcag ggtgccttgc cggtcatccg cgcccaggcc
      121 tggtcgagcg cggcatcggc ggcgcgatcc gccggcacca gccacatttt cacgccggcg
      181 agcttctcca gccgaaaatc ggtgcgcgcg tcgagccgca gcacgtccat gtgatcggat
      241 atctgggcaa tgaagggcag aaacagcgcc cggttcaggc cgcccttgta gagatcctcg
      301 ggcgcgacgt tggaggtcgc caccaccacg gtgccgagtt cgaacagctt tgagaacagc
      361 cgtcccagga tcatcgcatc cgcgatgtcg gtgacatgaa attcgtcgaa acacaaaagc
      421 caggcctcgt cgaaaatcga tccggcggtg agcgcgatca catcgccgtc ggcgatttcg
      481 ccgcgcgcga tattctgacg atagccatag atccgctcgt gcacttcggc catgaattcg
      541 tgaaaatgcg cgcggcgctt gtgctcgacc tggctgtgct ggaaaaacag gtccatcaac
      601 atggtcttgc cgcgcccgac atcgccatga acatagagcc cgcgcggcgg cgcgccgtcc
      661 ttgtcggcaa acagccggcc cagcaggcct tgcttgcgca aaggcttgta gcccgcgagc
      721 cgccgctcga ggtccgcgaa ggcgtcggcg gcctccgctt gcgcggcgtc gggttcgatc
      781 acgccggacg cgacgagggc ctgataatgg gcgctgaacg aggcggatgg agtggacagc
      841 agcatggccc cttttacggc cccaacgtta aggaaattgc aagccacgaa ttgatgcagg
      901 ggctatgcgg gggatgctcg gaaacaccgc gccgcctcat aacagaccgt catacccgcg
      961 aaaaattggc tgtgcaaaaa ctcaacacgg cgcgacggtg atagaatggg attccctcga
     1021 agctcatttc cggtccgcaa gattgcgcgt ctgatcgcta ttacttgttc caagaaagat
     1081 tattctcttc gtttatcgaa ttgtggagtt ttcacacagc caggaaaaag cgggtatcca
     1141 gtacgccgca gcttctcgat tccatcaccg acgtttcgga atactgggtc gcgcggtcag
     1201 gccgggcgaa gacgctggga atggggcacg cccacgcttc actcgctcag cgcgtaggaa
     1261 tcctcgacca catagggccc gccgccggtg gaggcccggg acgagaacag cacgaaccgc
     1321 gatgcggtga acaccctggt cggaaaatag ccgcgcaccg acagatagtc cgcgacatcc
     1381 tggttggagg cgtcacgcag ccgcgccagc gtcacatgcg gggtgaattt tcgcccttcg
     1441 ggatcgagac cgaaacgctg catcagccgc tccagttcgg cctgcagctc gatcagcggc
     1501 cggctcggcg cgatcgacgc gaccaccgcg cgcggcttgc gcccgccgaa actcgacagg
     1561 ccctgcaggg caacc
//
LOCUS       DASWGM010000156         9192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2371557,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000156 DASWGM010000000
VERSION     DASWGM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371557"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..2216)
                     /gene="glgB"
                     /locus_tag="VGA15_04740"
     CDS             complement(69..2216)
                     /gene="glgB"
                     /locus_tag="VGA15_04740"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX9587033.1"
                     /translation="MTKLSAEAYAIVEGRHSDPFHYLGLHTEGDRTVVRAFLPEASKV
                     DAIGEHGETATLARIHDAGLFAGALPNGSRRYQLRARFGENVVDLDDPYRFPPVLTDF
                     DLYLLGEGTHQRIYDKLGAHPMTLEGVDGVAFVVLAPNARRVSVVGDFNFWNARRHPM
                     RRRGVGYWELFVPHATSGDHYKFDIIGSQGQHLPLKSDPMAFAAEIRPKTASIVLDET
                     RLPHPRPSPANINALSAPMSIYEVHLGSWRRKGNNEWLTYRDLAEQLPRYARDLGFTH
                     IEFLPVSEHPFDGSWGYQPTGMYAPTSRFGSPADFSALVDACHREGLGVMLDWVPGHF
                     PDDPHGLGNFDGTALYEHANPLQGRHLDWGTLIYNYGRTEVVNFLVSNALFWLERYGI
                     DGLRVDAVASMLYLDYSRPGGGWIPNKYGGRENLEAIDFLRRFNTELFARFPQATTAA
                     EESTSWPQVSRPVEYGGLGFGYKWNMGWMHDTLNYIGKDPIHRKHHHGDILFGLHYAF
                     SENFILPLSHDEVVHGKRSLLGRMPGDDWQRFANLRAYYAFMFGHPGKKLMFMGCEFG
                     QEREWNHDRSLDWHLLEQKKYAGIQSLIRDLNGLYRTLPALHEMDCDQSGFEWVVTDD
                     AGGNVFAWIRKGNDARARCLVVVNFSPNVYYNYRVRVPFAGKWREVFNSDSAHYGGSN
                     VGNVGEVRTLDGIVPELNLTVPPLAAIFLIPES"
     gene            complement(2213..5518)
                     /gene="treS"
                     /locus_tag="VGA15_04745"
     CDS             complement(2213..5518)
                     /gene="treS"
                     /locus_tag="VGA15_04745"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX9587034.1"
                     /translation="MNLMSSIDENELPVTDLDGDELWYKDAIIYQLHVKAFADSNNDG
                     IGDFAGLTEKLGYLQDLGVTALWLLPFYPSPGRDDGYDIADYGAINPDFGTMKDFRRF
                     IVEAKRRGLRVITELVINHTSDQHDWFKRARRSDPDSSARNWYVWSDTDQKYADTRII
                     FTDTEKSNWTWDPEAGQFYWHRFFSHQPDLNFDNPRVVSALVQVMKRWLDTGVDGFRL
                     DAIPYLCERDGTNNENLPETHAIIRKLRAELDTYAKGKVLLAEANQWPEDVQEYFGRG
                     DECHMAYHFPLMPRIYMALAQEDRFPITDILRQTPDIPSNCQWALFLRNHDELTLEMV
                     TDVERDYLWSTYANDPRARINVGIRRRLAPLMDNDRRKIELMNSLLLSFPGTPIIYYG
                     DEIGMGDNIYLGDRNGVRTPMQWTPDRNGGFSRADPARLYAPTIMDPVYGYESVNVEA
                     QSRSLSSLLSATKRLISVRKSTLAFGRGTMAFIRPVNRSVLAYVRQHGDEVILCVANL
                     SRSAQATELNLSAWKDRIPLEMLGRTRFPPIGELPYMITLAPYGFYWFQLKERDKSEH
                     TEPSVVPEFETLVVPLGATWMSLARTRGVFERDVLPGQLARTRWYPERSAEAIHPTLT
                     SAIPFCIIGDNRPWLIFFEATQRGATARYVLPMQIEWVRFDRERYNPRAFAAVRQGAR
                     EGTLLDVATDQIFIALLLRNLGEALTVEEEGLRLEFRPTSKFSDTPIKPSEYIRAVET
                     EQSNSTALVDSDYVVKVYRKLETGINPEIEIGRFLTEVAGFANTPALLGSVELVEGDN
                     RSAVAIVHAFVQNQGDAWTVTSAYLDRFVDEQRLLAASEHPGESEEQVPYLRYMSQIG
                     RRVAEMHLALAARDEFADFAPQPSTPADVQLWTEGVMTRAERVFDALKQRRETIKEAD
                     RLLVDQILAQRANLHERLSALLPPDVDGLNIRHHGDFHLGQILIVKDDIFIIDFEGEP
                     RRPLSERRRKAPAARDVAGLIRSIDYSATAALERALKVAPDENGKLGAALAEWRERAA
                     AAFLAAYREIVTNQRLWPADPLAAEQMLNFFLLEKAMYEIEYELAHRPEWLRVPLTGM
                     IRILSQQQTEEAS"
     gene            complement(5526..7481)
                     /locus_tag="VGA15_04750"
     CDS             complement(5526..7481)
                     /locus_tag="VGA15_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX9587035.1"
                     /translation="MNKTTQTVESAATGGPFHIEDIYPLIDGGRFAVKRIVGERVEVW
                     ADIYRDGHDVTAASLVWRREQDREWRREPMVDRGNDRWSGSFVPDAPGRYVYAIEAWT
                     DEFATWRHGFELKQKAGSDLALDAIEGAGLLTRAPAGGPAAAAIILKQCEEYLQTGDA
                     ASLLTAELKDAMAESQFRPDLTRSPLFPFVADRPLARAGAWYEMVPRSQGRIPGQRGT
                     FQDCIARLPDIAAMGFNVVYLTPIHPIGHTNRKGRNNAVTAANSDPGSPYAIGAAEGG
                     HDAVHPELGTLEDFRAFVAACKRLEMEVALDFAVQCSPDHPWLKQHPQWFKRRPDGSM
                     RYAENPPKKYQDIVNPDFSSEDAGALWNALRDVALFWIAQGVKIFRVDNPHTKPFRFW
                     EWLIHDIQLRHPDVIFLAEAFTRPKLMKGLAKLGFTQSYTYFTWRTQKWELEQYLNEL
                     TAYPEHDFYRPNFFVNTPDILPYHLQSGETWMFKSRVALAATLSSTYGIYNGFELLEH
                     DPIPGKEEYLDSEKYEIKVRDWDMTGNIKPYIRDLNRVRHENAALQQTSQLRFIPIDD
                     GNVIGFVKESVDMTNTVVGTIALSRDVHEFWLPLVDVQVFVGGERRHVAAVENLITGE
                     RFPLEWGGIRLRIDPARDPALLFRCLA"
     gene            7683..>9192
                     /gene="malQ"
                     /locus_tag="VGA15_04755"
     CDS             7683..>9192
                     /gene="malQ"
                     /locus_tag="VGA15_04755"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX9587036.1"
                     /translation="MDLITKAKHLGIQTEFIDGQGHRRVTSAAALKIIVDALPVRVPH
                     RFLDQAVVVRSGQPSPTKLSQAAALPLRWKIMAGLEVIAQGETTDRVIVWPSDLAVGS
                     YRLYLTDAASITEKTPLIVAPPRAFGGDFDRCWLLAVQLYGVRSARNWGIGDFTDLGA
                     LIELASRLGADGVGLNPLHALFDDRPADCSPYSPNSRLFLNALYIDVERLPELQRSAF
                     AESCDTIARLRASDIVDYVAVAELKWRALRSAFDAFKAGPRTDRHAAFSRFRTERGAT
                     LSRYACFEVLRHKFNKPWWEWPTEWQQPNDARCAALHAGPDSIEIEFVKFVQWSADQQ
                     LQACRDLAARLGMKVGLYLDLAVGVQSDGFDAWNEQVAISRHLAVGAPPDALNTAGQN
                     WGLAGFNAAVLEIQSFRPYREMLRASMRYAGAIRLDHVLGLKRLYLVPYGFAADDGVY
                     VQMPFEALLAATAQESMKHRCVVIGEDLGTVPKGFREQMADWGIWSYQVMMFE"
BASE COUNT         1766 a         2713 c         2890 g         1823 t
ORIGIN      
        1 aaattagtac cccggccgtc ccagcttgcg ccgagccgtg aggacgttcc ggcagacaat
       61 cgcatgcgtt agctttccgg tataagaaat attgcggcga gaggcgggac cgtgagattg
      121 agttcgggca caatgccatc caacgtccgg acctcgccga cgttcccgac attgctgccg
      181 ccgtaatgcg cggaatccga attgaaaact tcgcgccact tgccggcgaa cggcacgcgg
      241 acgcggtagt tataataaac attcggcgaa aaattcacga ccacgaggca tcgcgcgcgg
      301 gcatcgttgc ctttgcggat ccaggcgaac acgttgccgc cggcgtcgtc ggtcacgacc
      361 cactcaaaac cggattgatc gcaatccatc tcatgcagcg ccggcaacgt tcgatagagc
      421 ccgttgagat cgcggatcag ggactggatg ccggcgtatt ttttttgctc gagcagatgc
      481 cagtccagcg agcggtcgtg gttccactcg cgctcctgac cgaattcgca gcccatgaac
      541 atcagcttct tgccgggatg tccgaacatg aaggcgtagt aagcgcgcag attggcaaag
      601 cgttgccagt cgtcgccggg catccggccc agcagcgagc gcttgccgtg gacgacttca
      661 tcatgcgaca gcggcaggat gaaattttcc gaaaacgcat agtgcaggcc gaacaggatg
      721 tcgccgtgat gatgcttgcg gtggatcgga tccttgccga tgtagttcag cgtatcgtgc
      781 atccagccca tgttccattt gtagccaaag ccgagcccgc catattcgac cgggcgcgac
      841 acctgcggcc atgaggtcga ttcttccgcg gccgtggtgg cctgcggaaa acgggcgaac
      901 agttcggtat tgaagcggcg caggaaatcg atggcctcga ggttttcgcg gccgccatat
      961 ttgttcggaa tccagcctcc gccgggtcgg ctataatcca gataaagcat cgaggcgacc
     1021 gcatcgacgc gcaagccgtc gatgccataa cgctccagcc aaaacagcgc gttcgacacc
     1081 aggaaattga cgacttcggt gcggccgtaa ttgtagatca gcgtgcccca gtcgagatgc
     1141 cggccctgca gcgggttggc gtgctcgtaa agtgctgtgc cgtcgaaatt gccgagacca
     1201 tggggatcgt cgggaaaatg tccgggcacc cagtccagca tcacgccgag accttcgcgg
     1261 tgacaggcat cgaccaacgc ggaaaaatcc gcggggctgc cgaaccggct ggtcggcgcg
     1321 tacatgccgg tcggctgata gccccaggag ccgtcaaacg gatgctcgct gaccggaaga
     1381 aattctatgt gggtaaagcc gagatcgcgg gcatagcgcg gaagctgctc ggcgagatcg
     1441 cgataggtca gccactcgtt gttgcccttg cgccgccacg aaccgaggtg gacctcgtag
     1501 atcgacatcg gcgcgctcag cgcgttgata ttggcaggag aggggcgcgg atgcggcagc
     1561 ctggtctcgt cgagaacaat cgacgcggtt ttggggcgta tttccgcagc gaacgccatc
     1621 gggtcggact tcagcggcag gtgctggccc tgcgagccga ttatatcgaa tttataatga
     1681 tcgcctgacg tggcgtgggg cacgaacagc tcccaatagc caacgccacg cctgcgcatc
     1741 ggatgccgcc gtgcattcca gaaattgaaa tcgccgacaa cgctgacccg ccgtgcgttg
     1801 ggcgcgagta ccacgaacgc cacgccgtcg acgccctcga gcgtcatcgg gtgcgcgccc
     1861 agcttgtcgt agatccgctg atgggtgcct tcgccgagca gatacaggtc gaagtctgtc
     1921 agcaccggcg gaaagcggta ggggtcgtcg agatcgacca cgttctcacc gaagcgggcg
     1981 cgcagctggt aacgcctgga tccgttcggc agcgcgccgg cgaacagccc ggcgtcatga
     2041 atccgagcca gcgtggctgt ttcgccatgt tcgccgatcg catcgacctt cgacgcctcc
     2101 ggcaggaacg cgcgcaccac ggtccggtcg ccctcggtgt gaaggccgag ataatgaaag
     2161 ggatcggagt gacggccttc gacgatcgca taagcctccg cggaaagttt agtcatgagg
     2221 cctcttcggt ctgctgctga gacaatattc taatcatccc ggtcagcggg acgcgcagcc
     2281 attcaggcct gtgggccagc tcgtattcga tctcgtacat ggctttttca agcagaaaga
     2341 aattcagcat ctgctcggcg gccagcggat cggccggcca cagacgctgg tttgtcacga
     2401 tctcccgata ggcggcaaga aacgccgcgg ccgcgcgttc gcgccattcc gcgagcgccg
     2461 cgccaagctt gccattctcg tccggcgcga ccttgagcgc gcgctccagc gcggcggtcg
     2521 ccgaatagtc gatcgaacgg atcaggccgg cgacatcgcg cgccgccggc gccttgcgcc
     2581 gccgttcgct cagcggccgg cgcggctcgc cctcgaaatc gatgatgaag atatcgtcct
     2641 tcacgatcag tatctgtccg agatggaagt cgccatggtg acggatattc aggccgtcga
     2701 catctggcgg aagcagcgcg ctcaagcgct catgcaggtt ggcgcgctgt gccaggattt
     2761 gatcgaccag cagccggtcg gcttccttga tggtttcgcg ccgttgcttc aatgcgtcga
     2821 agacacgttc ggcgcgggtc atcacaccct cggtccagag ctgcacgtcc gcaggagtgc
     2881 tcggttgcgg cgcaaaatcg gcaaattcgt cacgggcagc gagtgcaagg tgcatctcgg
     2941 cgacgcgtct tccgatttgc gacatgtaac gcagataggg gacctgctcc tcgctttcgc
     3001 cgggatgctc gctcgccgcc agcagacgct gttcatcgac aaaacgatcg agataggccg
     3061 atgtcaccgt ccaggcgtcg ccctgatttt ggacaaaggc atgaacgatc gcgaccgcgc
     3121 ttcggttgtc gccttcgacc agttcgacgc tgccgagcag cgcgggcgtg ttggcaaagc
     3181 ccgcaacctc ggtcagaaac cggccgatct cgatctcggg attgatgccg gtttcgagtt
     3241 ttcgatagac cttgaccaca tagtcgctat ccaccagcgc ggtgctgttg gattgttcgg
     3301 tttcgacggc gcggatatat tcggacggtt tgatcggcgt gtccgagaac ttgctggtag
     3361 gtctgaattc gaggcgcaag ccctcttcct cgacagtcaa ggcttcgccc aggttgcgca
     3421 gcagaagcgc gatgaagatc tggtcggtcg caacgtccag caatgttcct tcccgcgcac
     3481 cctgacgcac cgcggcaaat gcgcgggggt tatagcgctc gcgatcgaac cgcacccact
     3541 cgatctgcat cggcaatacg taacgcgcgg tcgcgccgcg ctgcgtcgct tcaaagaaga
     3601 tgagccaggg ccggttgtcg ccaataatgc aaaaagggat cgccgaggtc agtgtcggat
     3661 gaatagcctc tgccgaacgc tccggatacc agcgggttcg tgccagctgt cccggcaaga
     3721 catcgcgttc gaatacgccg cgggtgcggg ccagcgacat ccatgtcgcg cccagcggca
     3781 cgacgagggt ctcgaattcc ggaaccaccg acggttcggt gtgctctgac ttgtcgcgct
     3841 ccttgagctg gaaccagtag aagccgtatg gcgccagcgt gatcatgtag ggcaattcgc
     3901 cgatcggcgg gaaacgggtg cggccgagca tttccagggg aatgcgatcc ttccaggccg
     3961 aaaggttgag ttcggtggct tgcgccgatc gagacaggtt ggcgacgcag aggatcacct
     4021 cgtcgccatg ctgcctgaca taggcgagca ccgatcgatt gaccgggcgg atgaaggcca
     4081 tggtgccgcg gccgaacgca agcgtcgact ttcgcaccga gatcagccgc ttggtcgcac
     4141 tgagcagcga agacaggctg cgcgactggg cttcgacatt gaccgactca tagccgtaga
     4201 ccggatccat gatggtcggg gcgtaaagcc gcgccggatc ggcgcgggag aagccgccgt
     4261 tgcggtccgg cgtccattgc atcggcgtgc gcacgccgtt gcgatcgccg agatagatgt
     4321 tgtcgcccat gccgatctcg tcgccgtaat agatgatcgg cgtgcccgga aacgacagca
     4381 gcagcgagtt catcagctcg atcttgcgcc ggtcgttgtc catcagcggc gccagccggc
     4441 ggcggatgcc gacattgatg cgcgcgcgcg gatcgttggc gtaggtcgac cacagatagt
     4501 cgcgctcgac gtcggtgacc atttcgagcg tcagttcgtc gtggttgcgc agaaacagcg
     4561 cccactggca attgctcggg atgtccggcg tctggcgcag gatgtcggta atcggaaacc
     4621 gatcttcctg ggcgagcgcc atgtagatcc gcggcatcag cgggaaatga taggccatat
     4681 ggcattcgtc gccgcggccg aaatattcct gcacgtcctc cggccattga ttggcctccg
     4741 cgagcagcac cttgcccttg gcataggtgt cgagctcggc gcgcagcttc ctgatgatgg
     4801 cgtgggtttc cggcaggttc tcgttattgg taccgtcgcg ctcgcaaaga taggggatgg
     4861 cgtcgagacg gaaaccatcg accccggtgt cgagccagcg cttcatgacc tgcaccagcg
     4921 cgctgaccac gcgcggattg tcgaaattca gatccggctg atgcgagaag aagcggtgcc
     4981 agtaaaactg gccggcttcg ggatcccagg tccagttcga cttttcggta tcggtaaaga
     5041 tgatccgcgt gtccgcgtat ttctggtcgg tgtcgctcca tacataccag ttgcgcgcgc
     5101 tcgaatccgg atcgctgcgg cgggcgcgct tgaaccagtc gtgctggtcg gaggtgtgat
     5161 tgatgacgag ttcggtgatg acccgcaagc cccgccgctt ggcttcgacg atgaagcggc
     5221 ggaaatcctt catggtcccg aaatcggggt tgatggcgcc gtagtcggcg atgtcatagc
     5281 cgtcgtcgcg gccgggcgac ggatagaacg gcagcagcca cagcgcggtg acgccaaggt
     5341 cctgcagata gcctagcttc tcggtcaatc ctgcgaaatc gccgatgccg tcattgttgc
     5401 tgtcggcaaa ggccttgacg tgaagctggt agatgatggc atctttgtac caaagctcgt
     5461 caccgtcgag gtcggtgacc ggcaattcgt tttcgtcgat cgatgacatg aggttcatgg
     5521 gaccctcacg ccagacagcg aaacagcagc gcgggatcgc gcgccgggtc gatccgcagg
     5581 cgaatgccgc cccactcgag ggggaatcgc tcgccggtga tcaggttctc aactgcggcg
     5641 acatggcggc gttctccgcc gacgaagacc tggacgtcga ccagcggaag ccagaattca
     5701 tgtacatcgc gtgacagcgc gattgttccc acaacagtgt tggtcatatc gaccgattcc
     5761 ttgacaaagc cgatgacgtt gccgtcatcg atcggaatga agcgcagttg gctggtctgc
     5821 tgcagcgctg cgttttcgtg ccggacccga ttgagatcgc ggatgtaggg cttgatatta
     5881 cccgtcatgt cccagtcgcg caccttgatc tcgtattttt cggaatcaag atattcttcc
     5941 ttgccgggga tcggatcgtg ttcgagcagc tcgaatccgt tgtagatgcc ataagtgctc
     6001 gacaaggtgg ctgcaagcgc gacgcgggac ttgaacatcc aggtctcgcc gctttgcaaa
     6061 tgatagggca ggatatcggg cgtattgacg aagaagttcg ggcgatagaa atcgtgctcc
     6121 ggataggccg tcaactcgtt cagatactgc tccagctccc acttctgcgt acgccaggta
     6181 aagtaggtgt aggattgcgt aaagccgagt ttcgcaaggc ccttcatcag tttcggccgg
     6241 gtaaaggctt cggcgaggaa gatgacatcg ggatggcgaa gctgaatgtc atgaatcagc
     6301 cattcccaga accgaaacgg cttggtgtgg gggttgtcga cccgaaaaat tttgacgcct
     6361 tgcgcgatcc agaacagcgc cacatcgcgc agggcattcc acagcgcgcc ggcgtcttcc
     6421 gacgaaaaat cggggttgac gatatcctgg tatttcttgg gcggattttc cgcatagcgc
     6481 atcgagccgt ccggccggcg cttgaaccat tgcggatgct gtttgagcca gggatgatcg
     6541 ggcgaacact gaaccgcgaa gtcgagcgcg acttccatct ccagacgctt gcatgccgca
     6601 acgaacgcac ggaaatcctc cagcgtgccc aattccggat gcaccgcatc gtggccgccc
     6661 tcggcggcgc cgatggcgta gggactcccg ggatcgctgt tggcggccgt gaccgcattg
     6721 ttgcggccct tgcggttagt gtggccgatg gggtggatcg gcgtgagata gaccacgttg
     6781 aatcccatcg ccgcgatatc cggcaagcgc gcgatgcaat cctggaaggt gccgcgctgg
     6841 cccggtatgc ggccctggct gcgcggcacc atctcgtacc aggcaccggc ccgcgccaga
     6901 ggccgatccg cgacaaatgg aaacaacggc gatcgcgtca ggtcgggccg aaactggctc
     6961 tcggccatgg cgtccttcaa ttcggccgtc agcagcgacg cggcgtcgcc ggtctgaagg
     7021 tattcctcgc attgcttcaa gatgatggcg gccgcggccg gtccgccggc cggcgccctg
     7081 gtgagcaggc ccgcgccctc gatcgcgtcg agggccaggt cggagccggc tttttgtttg
     7141 agctcaaatc cgtgccgcca ggtcgcaaat tcatcggtcc atgcctcgat cgcatagaca
     7201 tagcgtccgg gtgcgtccgg gacgaacgaa ccgctccagc ggtcattgcc gcggtcgacc
     7261 atcggctcgc gacgccactc ccgatcctgt tcgcggcgcc agaccagcga cgcggccgtc
     7321 acatcatggc cgtcgcgata gatgtctgcc cagacttcga cacgctcccc gacgatacgc
     7381 ttcacagcaa agcggccgcc gtcgatcagc ggatagatat cttcaatatg gaaggggccg
     7441 ccggttgcag cgctctcgac agtttgagtt gttttgttca cggtgatgcc attgacatgg
     7501 aaaagacgtc ccgtttcgtt tgcgggaaag gagacgctcc agacctatat accaaagccg
     7561 ctcatcgggt attggttccc cgggaacggc ggcgacgccg gagagttaaa cacggctatc
     7621 ctattcctgc atctagagaa tttcgcgacg tctctcggcg ttgcgtgcaa accgcgtgcc
     7681 gaatggacct tatcaccaaa gctaaacacc tcggaatcca gaccgaattt atcgacggtc
     7741 agggtcaccg ccgtgtgacg agtgccgcgg cgctgaaaat catcgtcgat gcgttgccgg
     7801 ttcgagtacc gcaccggttt ctcgaccagg cggtggtggt tcggtccggt cagccatcgc
     7861 cgactaagct cagccaagcc gctgccttgc cgctgcgctg gaaaatcatg gcgggccttg
     7921 aggttatcgc gcaaggcgag accaccgatc gcgtcatcgt ctggccgtca gatttggctg
     7981 tgggttcgta ccggctgtat ttgaccgatg ccgcctcgat taccgaaaag acgccgttga
     8041 ttgtggcgcc gccaagggct ttcggcggcg atttcgaccg ctgctggtta ttggcggtac
     8101 agctctacgg cgtccggtcg gcgcgcaatt gggggatcgg ggatttcacc gacctcggag
     8161 cgttgatcga actcgccagc cgattgggag cggatggcgt cggccttaat ccgttgcatg
     8221 cgctattcga tgaccgtcct gccgattgca gcccgtattc tccgaacagc cggctgtttt
     8281 tgaatgcgct ctatatcgac gttgagagat tgccggaatt gcagcgcagt gctttcgctg
     8341 aaagctgtga caccatcgcc agactgagag cgagcgatat cgtcgattat gtcgccgtcg
     8401 ccgaactgaa gtggcgcgcg ctgcgatcgg cgtttgacgc cttcaaggct ggtcccagga
     8461 cggatcgtca tgcggcgttc tcaaggtttc gcaccgagcg cggggcaacg ctttcgaggt
     8521 acgcctgctt cgaggtcctg cggcataaat tcaacaaacc gtggtgggaa tggccgacgg
     8581 agtggcagca gccgaacgat gccagatgcg ctgcccttca cgcgggccct gattcgatcg
     8641 agattgagtt cgtcaaattc gtgcaatggt ctgccgatca gcagttgcag gcttgccggg
     8701 atctcgccgc caggctgggc atgaaggtcg gtctctacct cgatctggcg gtcggcgtgc
     8761 aatccgacgg gttcgatgcc tggaacgagc aggtcgcgat ttcccgccat ctcgcggtgg
     8821 gcgcgccacc ggatgcactt aataccgccg gtcagaattg gggcctcgcc ggcttcaacg
     8881 ccgccgtact cgaaatccag tccttccggc cgtatcggga aatgctgcgg gcttcgatgc
     8941 gctacgccgg cgcgatccgg ctcgatcatg tccttggtct gaagcggctt tatctggtgc
     9001 cgtacggctt tgccgccgac gacggcgtct atgtgcaaat gcctttcgag gcgctgctgg
     9061 cggcgaccgc acaggaaagc atgaagcatc gatgcgtggt gatcggcgaa gatctcggca
     9121 ccgtgccgaa aggctttcgc gagcaaatgg ccgattgggg catctggtcg tatcaggtga
     9181 tgatgttcga gc
//
LOCUS       DASWGM010000157         5783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2232605,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000157 DASWGM010000000
VERSION     DASWGM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232605"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VGA15_04760"
     CDS             complement(<1..906)
                     /locus_tag="VGA15_04760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX9587037.1"
                     /translation="MVYAANNSVQGAKKDEGGFDGDAPTAILIEAGSGSVLFEKNADE
                     LRAPSSMMTLMTAETVFHAIKQGEVKLTDEYHVSENAWRKGGAPAGGPTMFAAIHSKI
                     SVDDLLHGAIIQNGNDACMVLAEGIAGNERIFADMMTKRAREIGLTQSIFANASGLPD
                     PGNKMTVRELAKLARFIVQTYPDFYKLFSEREFTWNKIRQQSRNPLLLSLEGADGLKT
                     GYTKEGGYGMVASAVQNGMRLIVVINGLDDPDDRVSEAKKMLEWGFRNFEARTLFAAQ
                     QPVGYAKVFGGESRSVKLASSEPIKV"
     gene            1188..2147
                     /locus_tag="VGA15_04765"
     CDS             1188..2147
                     /locus_tag="VGA15_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587038.1"
                     /translation="MPSSRTVSANGIEIFVLEQGEGPLVLFCHGWPELSYSWRHQIPA
                     IAAAGFHVAAPDMRGFGRTSAPADINAYSIFDHVGDMVALVAALGKNQAVIVGHDWGA
                     PVAWHAAMFRPDIFTAVAGLSVPPPFRGRGRPLDSLRESGITNFYWQYFQQPGIAEAE
                     FERDVGLTMRTLLGRGFSDPAASLFVEEGKGFLSEPPADRPLPDWLGETDLTYFTEAY
                     QKSGFRGGLNWYRNIDRNWELTAPWQGAQIHQPSLFIAGSNDSVITGLIGAKRVADME
                     RVLPNLRQKLIIGGAGHWIQQECADAVNAALIAFLKDNTGAAQ"
     gene            complement(2141..3079)
                     /locus_tag="VGA15_04770"
     CDS             complement(2141..3079)
                     /locus_tag="VGA15_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX9587039.1"
                     /translation="MGIRRSDPVLLIARRAAAVAACLVLANCASSGKFASRVDPKYGV
                     SSSPRVVEFGEPVPKGGGTYRVGKPYTVGGRIYVPEEDTNYREEGMASWYGDDFHGRL
                     TANGEVFDMGSLTAAHPTLPMPCYARVTNLSNGKSLIVRVNDRGPYHGNRLIDVSNKA
                     AELLEFKGVGVARVRVEYVARAPLEGSDDRQLMATLRTGEPAPSPSLVRVASARAFIP
                     EISSSARPIRGEVPMPEGRPYSLGNTSADMASMSATSEMSASGRVRSRGRIIDNPRAV
                     SYENAGAYAPDVGPVSAYAPIDPQGPRELLAGRGLY"
     gene            complement(3293..3511)
                     /locus_tag="VGA15_04775"
     CDS             complement(3293..3511)
                     /locus_tag="VGA15_04775"
                     /inference="COORDINATES: protein motif:HMM:NF021362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEX9587040.1"
                     /translation="MILFQGAETHTRSVLKALSWRTLGTLDTFVISWFLTGKLELAGL
                     IAGFEFITKIGWYYLHERVWASIAWGRR"
     gene            3664..3906
                     /locus_tag="VGA15_04780"
     CDS             3664..3906
                     /locus_tag="VGA15_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587041.1"
                     /translation="MSKPSLDGRHRDKNGEISHKHGNTLIRTLRKIYGPGFAAGYPET
                     EKLSEVLLQLNETSLSQLRRDHETGHLEHKIAQASK"
     gene            4084..4173
                     /locus_tag="VGA15_04785"
     tRNA            4084..4173
                     /locus_tag="VGA15_04785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4118..4120,aa:Ser,seq:tga)
     gene            complement(4353..4883)
                     /locus_tag="VGA15_04790"
     CDS             complement(4353..4883)
                     /locus_tag="VGA15_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9587042.1"
                     /translation="MTSEIVFRLGFEVEDADCLQQAAATLERLGHRVQGGSADDAARR
                     KVRAFIDFEDPSGNHIELVVRPECSGRRYFAARDAGIQHVNHQVQTSDDVLRSFYFLS
                     ERRVPIVFGPGRHPTSGARFLYFTGPDGMVFEYSVGVDEIEDEATHRPRQFGFEPTSF
                     CMWGSKPAGMALPNEQ"
     gene            5076..>5783
                     /locus_tag="VGA15_04795"
     CDS             5076..>5783
                     /locus_tag="VGA15_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9587043.1"
                     /translation="MQKVQVENNADGTTLITINRPERRNAICAETALMLQKAFQDFER
                     SSTQKAAVLTGAGNEAFSGGADTGNFPELWRCIPTVGFETNKPIISAVGGWCVGGALV
                     LSMMCDLTVAAENAKFSYPEARLGFTGGLIAGLAARIPHKVAMEMILLCRILDPQRAY
                     NIGLINEVVPTGQQVQVALKMAREMAEFSPLVLKTLKRFVTEGVLAQGPSEQSGRAQR
                     ALYEVKESEDADEARSAV"
BASE COUNT         1162 a         1797 c         1723 g         1101 t
ORIGIN      
        1 caccttgatc ggctcggagc tggccagctt gaccgagcgg ctttcgccgc cgaacacctt
       61 ggcgtagccg accggctgct gggccgcgaa caatgtccgc gcctcgaaat tgcgaaatcc
      121 ccattccagc atcttcttcg cctcggagac acgatcgtcc ggatcatcga ggccgttgat
      181 gacgacgatc agccgcatgc cattctgcac cgcggaggcg accatgccgt agccgccctc
      241 cttggtatat ccggtcttca gtccgtcggc gccctcaagc gaaagcagca gcggattgcg
      301 gctctgctgc cggatcttgt tccaggtgaa ttcgcgctcg ctaaacagtt tgtagaaatc
      361 gggataggtc tggacgataa agcgcgcgag ctttgcgagt tcacgcaccg tcatcttgtt
      421 gccgggatcg ggcaggccgc tggcattggc gaagatcgac tgcgtcaacc cgatctctcg
      481 cgcgcgcttg gtcatcatgt cggcgaagat gcgctcattg ccggcgatgc cctccgccag
      541 caccatgcag gcatcgttgc cgttctggat gatggcgccg tgcaggagat cgtccaccga
      601 aatcttgctg tgaatggcag cgaacatcgt cggcccgccc gcgggcgcgc cgcccttgcg
      661 ccaggcgttt tcgctgacat gatattcatc ggtcagcttg acctcaccct gcttgatggc
      721 gtgaaacacg gtttcagcgg tcatcaacgt catcatgctg gatggcgccc gcaactcatc
      781 tgcgttcttt tcgaacagca cgctgccgct accggcttcg atcaggatcg cggtcggcgc
      841 gtcgccgtcg aaaccgccct cgtctttctt ggcgccctgc acgctgttgt tggcggcgta
      901 caccacgccg ctgcacccga cagcgaccgc gacaaaggct gcgatcaggc cgcgccaggc
      961 gcgtcctgat ccggccgtcg gcttgcggga aactgatatt tcgactgcca tcggcgatgc
     1021 cctgagccgg cttttccgga tcatcatcta acagctggaa gtatcgcaaa caacacgggt
     1081 tttacccgct gccgcggagc cgatcggatt gcggcgctgg tcaaacgccc ctatcgtgtc
     1141 ggcttaccgg ttccccggca aaatccccga aacaaggcgg aaaagcgatg ccttcctcac
     1201 gcacggtttc cgccaacggg atcgagatat ttgtgcttga gcagggcgaa ggcccgctgg
     1261 ttctgttttg ccatggctgg ccggaattgt cctattcctg gcggcatcaa atcccggcga
     1321 ttgcggcggc gggcttccat gtcgcagcgc ccgatatgcg gggttttggc cgcaccagcg
     1381 cccccgccga tatcaatgcg tacagtattt tcgatcacgt cggggacatg gtcgcgctgg
     1441 tcgcggcgct cggaaaaaac caggccgtca ttgtcggcca tgattggggc gcccccgtcg
     1501 cctggcacgc ggcgatgttc cgcccggata ttttcacagc cgtcgccggc ctgagcgttc
     1561 cgccgccgtt ccgtggccga ggccggccgc tcgatagctt gcgcgaaagc gggattacca
     1621 atttctactg gcaatatttt caacagcccg gcatagccga ggccgagttc gagcgcgatg
     1681 tcggcctgac gatgcgaacc ctgctcgggc gcggattttc cgatccggcg gcctcgctgt
     1741 ttgtcgaaga aggcaaggga tttctcagcg agccacctgc ggatcggccg ttgccggatt
     1801 ggcttggcga aaccgacctc acctatttca ccgaggccta ccaaaaatcc ggcttccgcg
     1861 gcgggctcaa ctggtatcgc aacatcgatc gcaactggga actgaccgcg ccatggcaag
     1921 gcgcgcaaat ccatcagccg tcactgttca ttgcgggatc gaatgattcc gtcatcaccg
     1981 gccttatcgg cgccaaacgc gtcgccgaca tggaacgggt gctgccgaac ttgcgacaaa
     2041 agctcatcat cggcggcgcc ggccactgga ttcagcagga atgcgccgac gcagtcaacg
     2101 ccgccttgat cgcctttctc aaggacaaca caggcgcggc tcaatagagc ccgcggccgg
     2161 ccagcaattc gcggggaccc tgcgggtcga tcggcgcata ggcggaaaca ggcccgacat
     2221 caggcgcata agccccggca ttttcatagg aaactgcgcg cggattgtcg atgatgcggc
     2281 cgcgcgaacg cacgcggccc gaggcggaca tttcggacgt cgctgacatc gacgccatgt
     2341 ctgccgaggt attgccgagg ctgtaaggcc gcccttccgg catcggaacc tcaccccgga
     2401 tcgggcgggc agaggaggaa atttccggaa tgaacgcgcg ggcggacgct acccggacca
     2461 gggatggcga cggcgccggc tcgccggtgc gcaaggtcgc catgagctgg cggtcgtcgg
     2521 aaccttccag cggcgcccgg gcgacatatt ccacccgcac ccgcgcgaca ccaacgcctt
     2581 tgaattcaag cagttcggcg gccttgttgg aaacgtcgat aagccggtta ccgtggtacg
     2641 gcccccggtc attgacacgg acgatcagcg atttgccgtt actgaggttg gtcacgcgtg
     2701 cgtaacacgg catcgggagg gtcggatggg cggcggtcag cgaccccatg tcgaacactt
     2761 cgccgttggc ggtcagccgg ccgtggaaat catcgccata ccaggaggcc atgccctcct
     2821 cgcgataatt ggtgtcttct tccgggacat agattcgccc accgacggta tacggcttgc
     2881 cgacgcggta ggtgccaccg cccttcggca ccggttcgcc gaactcgacc acccgggggc
     2941 tcgaggaaac gccatatttg ggatcaaccc ggctggcaaa cttgccggac gaggcacagt
     3001 tggcgagcac gaggcacgcg gccaccgcgg cagcacgccg cgctatcagc aaaaccggat
     3061 ctgatcgccg aatccccata cgccccaact accgttccac cggtcgcgcg gcacttcaag
     3121 acgccaacga ccaaacgccc cgatgcagcc cacctccgca tcaaggtaac gaaaagatat
     3181 cgcaacccga acacggcgga aatggggaga gtacatcggt atggtgaatc gatggttgcc
     3241 ggctcaccgt tgaattacgg agcagccgga tttgagccag cgacaaggat cgtcaacgac
     3301 ggccccaggc gatggacgcc cagacgcgct cgtgaaggta ataccacccg attttggtga
     3361 tgaactcgaa gccggcgatc aagcccgcca gctccagctt gcccgtcagg aaccagctga
     3421 tcacgaaggt gtcgagcgtt cccaatgtcc gccagctgag ggctttaagg accgacctgg
     3481 tgtgtgtctc agctccctgg aatagaatca cccggtgcgc ctccctgctt gacggtcatc
     3541 gacaatttca cgcgggcatc ctgctgcctg ccgtcgttca tcgggccgat cacgcaactc
     3601 caccacttgc catgacgccc cgccgggaag atgctgccta caggggaaac agaggagcgc
     3661 agcatgagca agccaagcct cgacggacgt caccgcgaca agaacggcga aatcagccac
     3721 aagcacggca acacactgat ccgtacctta cgcaaaatct acggaccggg ctttgcggcc
     3781 ggttatcccg aaaccgaaaa actcagcgag gtactgctcc agctcaacga gacgtcgctg
     3841 agccagcttc gccgcgatca cgagaccggc catctggaac acaagatcgc ccaagcctcg
     3901 aagtaacaag ccctcgaagc taagtaataa gcctccaagt aggacgccct cgccccgcgc
     3961 gcgttgacga agccctgcgg gctgccataa acaggcgcac ctgagcgttg gatagcaaag
     4021 cagtagcaaa cggcctttcg ggcctcaagc gcctcttaag tcgctaaaca acttcaatgg
     4081 tttggaaggg tggccgagtg gtttaaggca ccggtcttga aaaccggcgt gcctgcaagg
     4141 gtaccgtggg ttcgaatccc accccttccg ccagtttgtt gttctcccat gttcgccgtt
     4201 cttcggaaga gcaaataaaa tcaacgcgtt atgtggctga gtggttttat tgttaaccgg
     4261 cattcgccag agttgggagc gttgaccgtg gcttttaccg taagtttggg acggtttggt
     4321 cgcctgatgg cgtcgcgccg atgctggtct tcttattgtt cgttgggcaa agccatgcca
     4381 gccggcttcg aaccccacat gcagaagctg gtcggctcaa aaccgaactg ccgcggccga
     4441 tgggtcgcct cgtcctcgat ctcgtcgaca ccgacggaat attcgaacac catcccgtcg
     4501 ggccccgtga agtagaggaa gcgcgcaccg gaggtcggat ggcggccggg tccgaaaaca
     4561 atcggcacgc gacgctcgct tagaaaataa aatgatcgca gcacatcgtc gctggtttgc
     4621 acctgatgat tgacgtgctg aatgccggca tcccgtgcgg cgaagtagcg gcggccgcta
     4681 cactcggggc gcaccacaag ctcgatatga tttccgctcg gatcctcaaa atcgatgaag
     4741 gccctcacct ttcgcctggc ggcgtcgtcc gcggagccgc cctgtacgcg atggccgagc
     4801 cgctccagtg tggcggcggc ttgctgcaaa cagtctgcgt cttcgacctc gaacccgagc
     4861 cggaagacta tttcagacgt cacccttgct gtcgttagcg gcggctatca cgatcagcat
     4921 atcgggggat gttggtcacc gtcggaatag aaatccgcta cgcgcggtaa ggctggtagc
     4981 cgaggccggg aaaatccggg gaatttgttc tccccctgtt caggtgccag cgatctcgct
     5041 tcgtctgctc cacggattgt tgaaagggaa taaccatgca aaaggtccag gtcgagaaca
     5101 acgccgacgg aaccacactg atcacgatca accgcccgga acgccgcaac gcgatttgtg
     5161 ccgagacggc gctgatgctg caaaaagcgt ttcaggattt cgagcgctcc agcacacaga
     5221 aggctgcggt gctcaccgga gccggcaacg aagcgttttc cggtggcgct gataccggca
     5281 attttccgga actctggcgc tgcattccga ccgtcggctt tgagaccaac aagccgatta
     5341 tttccgcggt aggcggctgg tgtgtcggcg gcgctcttgt gctctcgatg atgtgcgatc
     5401 tcacggttgc tgcggagaat gccaagtttt cctaccccga agcgcgattg ggttttacgg
     5461 gtggcctgat cgctggactg gcggcgcgca ttccgcataa ggtcgcgatg gaaatgatac
     5521 tgttgtgccg tatcctcgat ccgcaacgcg cctacaatat cggcctcatc aacgaggttg
     5581 tgccgacagg acagcaggtc caggtcgcgc tcaaaatggc tcgcgaaatg gccgagttct
     5641 ctccgctggt gctgaagaca ttgaagcgtt ttgtaacgga aggtgttttg gcgcagggac
     5701 cgtccgaaca atccggtcgg gcacagcgcg cactctacga ggtcaaggaa agtgaagacg
     5761 ccgacgaggc caggtcagcg gtg
//
LOCUS       DASWGM010000158         4235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2232658,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000158 DASWGM010000000
VERSION     DASWGM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232658"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VGA15_04800"
     CDS             complement(<1..822)
                     /locus_tag="VGA15_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEX9587044.1"
                     /translation="MRTKVAIIGAGPAGLLLGQLLHAYGIDNVILERQTPDYVLGRIR
                     AGLLEEGTVALLDEVGAGARAHREGLVHHGVELAFGGARHRIDMHAATGKTVMIYGQT
                     EVTLDLMNVRKAAGLTTIYQATDVAPHDFDTDHPRVSYVSDGVTCEIACDFIAGCDGF
                     HGVSRASVKPSAIETFERVYPFGWLGILSETPPVSHELIYSNHARGFALCTMRSMHRS
                     RYYVQCSLDDHVDQWPDERFWDELKRRLDQKAVDNLVTGPSIEKSIAPLRSFVAEP"
     gene            963..1991
                     /locus_tag="VGA15_04805"
     CDS             963..1991
                     /locus_tag="VGA15_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9587045.1"
                     /translation="MRKTFLATFLALLLAVGVTPAVAQEKTFELKLSHWVPASHPLQK
                     ALEDWGAAVEKESGGTIHYKVYPAQQLGKAFDHYDMARDGIADVTYVNPGYQPGRFPI
                     ISAGELPFLMSDAKGGSMALDAWYRKYAEKEMKDVKFCLAFIHSPSSFHSRTRKILVP
                     DDVKGMKIRPAHATMANFVTQLGGTNVQSSAPEVRDIIERGVADAVTFPWGSLVLFGI
                     DKVTKYDMDAPLYVTTFAFVMNKDKYNAMSDRQKKAIDDNCNTEAAGRVGEPWGKFED
                     AGLDKVKAEEGHEVYKLTPDQMLLWKKASEPLVKTWAEGVKKNGIDPDAAMAELRTAL
                     AKYNALTQ"
     gene            2072..2581
                     /locus_tag="VGA15_04810"
     CDS             2072..2581
                     /locus_tag="VGA15_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9587046.1"
                     /translation="MNRASMDRFIDTIEWIAAGFVGIVALNIFVGVLLRNTLNTAIPD
                     SFDIGRLLLGILIFWGIAATSYRGGHITVDLIWANVGPKYQRMIDVFATLVLLFVVTV
                     QTWTLFDKVRGTYNDNVLTFEMHMPTWPFFAVAWAGDVSAVLLIAIRTYRLIFNPDLM
                     QDPKIKATE"
     gene            2588..3904
                     /locus_tag="VGA15_04815"
     CDS             2588..3904
                     /locus_tag="VGA15_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX9587047.1"
                     /translation="MSTDTVAILGFVALFALMLLRVPVGMAMGLVGVTGFGYLNGFSP
                     ALKLVGQTSMRTVTDYTFGVIPMFLLMGTFVSNSGMSRELFRAANGFVGHLRGGLGIA
                     TVAACGGFAAICGSSVATAATFSAVAYPEMRRFGYPQSFATGVIAAGGTLGAMLPPST
                     VLAVYGIITEQDIGKLFIAGIIPGILAMTMYMITISLIGYLRPGFLPTGPQTSWRERF
                     AGLKNIWAPVLLFVFVIGGLYGLPFLPRFTPTEAGGVGATGAFLIGLFTGRLDKEKIL
                     ASLLQATRTAAAVFTVLIGALIFGYFLTVTQTPQKVTEFLTGLGLGSYGVLALIMVMY
                     LVLGCLMDAMAMIILTVPIIFPVITHLGFDPIWFGVIIVMTVELGLIHPPVGMNVFVI
                     KSVVKDVSFATIFRGVIPFVATDLIRLVILILFPLLATWLPQRMMG"
     gene            3994..>4235
                     /locus_tag="VGA15_04820"
     CDS             3994..>4235
                     /locus_tag="VGA15_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HEX9587048.1"
                     /translation="MVTPQLSTRYVFTITANIGSVTSAGDVGHGVRRIIPITGGDVRG
                     DINGKVCAFGADFQIIRPNELIELEAKYAFETDDGAV"
BASE COUNT          789 a         1268 c         1297 g          881 t
ORIGIN      
        1 cggctcggcc acgaaactgc gtaagggcgc gatgctcttt tcgatcgaag gcccggtgac
       61 gagattgtcg accgccttct gatcgagccg ccgcttcaat tcatcccaga atctttcgtc
      121 cggccattga tcgacgtgat cgtccaggct gcattgcacg taataccggc tgcggtgcat
      181 cgaacgcatg gtgcaaagcg caaaccctct ggcgtggttg gagtagatca attcgtggct
      241 gaccggcggc gtctccgaca ggattccgag ccagccgaac ggatagaccc gttcaaaggt
      301 ctcgatcgcc gacggcttca cgctcgcgcg gctgacgcca tgaaatccgt cgcacccggc
      361 gatgaaatcg caggcgatct cgcaggtgac gccatcgctg acataggaaa cgcggggatg
      421 gtcggtgtca aaatcatgcg gcgcgacatc ggtggcctga tagatcgtgg tgagaccggc
      481 ggccttgcgc acgttcatta gatcgagcgt gacttcggtc tggccgtaga tcatgaccgt
      541 cttgccggtg gcggcatgca tatcgatgcg atggcgcgcg ccgccgaagg ccagttcgac
      601 accgtggtgc accaggccct cgcggtgtgc gcgcgcgccg gcgcccactt catcgagcag
      661 agcgaccgtt ccctcctcga gcaggccggc gcggatgcgg ccgagcacat aatccggcgt
      721 ctggcgttcc aggatgacgt tgtcgatccc gtaggcgtgg agcagttgcc ccagcagcaa
      781 tcccgcaggc ccggcaccga tgattgctac ttttgtccgc aatacttgcc tccctggttg
      841 ttataggctt gaacgtgagc cgctgcggtc gtttccgcac gtacttgcaa tgataattat
      901 atcatataac aatatccgca aagggcattg aagcccggat ttcgcgaggg gagaagatgt
      961 cgatgaggaa aacgttcttg gcaacgttct tggcgctgct gctggccgtc ggtgtgacgc
     1021 ctgcggtagc gcaggaaaaa acctttgaac tgaagctgtc gcactgggtg ccggcgtcgc
     1081 atccgctgca aaaggcgctg gaggactggg gtgctgcggt ggaaaaggaa tccggcggta
     1141 ctatccacta caaggtttat cccgcgcagc agcttggcaa ggcgttcgac cattacgaca
     1201 tggcgcgcga cggcatcgcc gacgtcacct atgtcaaccc gggctatcag cctggccgtt
     1261 ttccgattat cagcgccggt gaacttccat tcctgatgtc cgacgccaag ggcggctcga
     1321 tggcgctcga cgcctggtat cggaaatatg ccgagaagga gatgaaggac gtcaagttct
     1381 gcctggcgtt catccattcg ccgtcgtcgt tccactcacg caccaggaaa atcctggtgc
     1441 cggatgatgt gaagggcatg aagatccgcc cggcgcacgc caccatggcg aatttcgtga
     1501 cccagctcgg cggcaccaac gtgcagtcct cggcgccgga agtccgcgac atcatcgagc
     1561 gcggcgttgc cgacgccgtc accttccctt ggggatcgct ggtgctgttc ggcatcgaca
     1621 aggtgacgaa atacgacatg gatgcgccgc tctacgtcac gacgtttgcc ttcgtgatga
     1681 acaaggacaa atacaacgcg atgtccgacc ggcagaaaaa ggcgatcgac gacaactgca
     1741 acaccgaagc cgccggccgt gtcggcgaac cctggggcaa gttcgaagac gccggtctcg
     1801 acaaggtgaa ggccgaggaa ggccacgagg tctacaagct gacgccggat cagatgctgc
     1861 tgtggaaaaa agcgtccgag ccgctggtca agacctgggc cgagggcgtc aagaaaaacg
     1921 gcatcgatcc ggacgcggcg atggccgagc tgcgtaccgc gctcgccaaa tacaacgcct
     1981 tgacccagta aagacctgat gcacctcggc acggcggcgg gatcctcctg ccgccgtgtt
     2041 ttcctgcgat cgccagggat gtggagcgga tatgaatcgc gcgtctatgg atcgtttcat
     2101 cgatacgatc gaatggatcg cggctggatt cgtgggcatt gttgccctca atattttcgt
     2161 aggcgtcctg ttacgcaaca ccttgaacac tgcgatcccg gattcgttcg atatcgggcg
     2221 cctgctgctt gggattttga tcttctgggg catcgccgcc accagctacc gcggcggaca
     2281 tatcaccgtc gatctgatct gggccaatgt cggcccgaaa taccagcgca tgatcgatgt
     2341 gtttgcgacg ctggtgctgc tgttcgtcgt gacggtgcag acctggacgc tgttcgacaa
     2401 ggtccgtggc acctacaacg acaatgtcct gacatttgaa atgcacatgc cgacctggcc
     2461 gtttttcgcg gtcgcgtggg ccggcgacgt ctccgcggtg ctgctgatcg caatccgcac
     2521 ctaccggctg atcttcaacc cggatttgat gcaggatccc aagatcaagg cgaccgagta
     2581 gaccggaatg agtaccgaca ccgtcgccat cctgggattc gtcgcgctgt tcgcgctgat
     2641 gctgttgcgc gtgccggtag gcatggcgat gggcctcgtc ggcgtcaccg gcttcggtta
     2701 cctcaacggt ttctcgccgg cgctcaaact ggtcggccag acctcgatgc gcacggtgac
     2761 ggactatacc ttcggcgtga ttccgatgtt cctgctgatg gggacattcg tcagcaattc
     2821 cggcatgagc cgcgaactgt tccgcgccgc caacggcttt gtcgggcatt tgcgcggcgg
     2881 gctggggatt gccaccgtcg cggcttgcgg cggctttgcc gcgatctgcg gctcgtcggt
     2941 ggcgaccgcc gcgacatttt cggcggtggc ctatccggaa atgcggcggt tcggctatcc
     3001 gcaatccttc gccaccggcg tgatcgcggc cggcgggacg ctgggcgcca tgctgccgcc
     3061 gtcgacggtg ctggcggtgt acggcatcat caccgagcag gacatcggca aactgttcat
     3121 cgccggcatc attcccggca tactcgccat gaccatgtac atgattacca tttcgctgat
     3181 cggttatttg cgacccggtt tcttgccgac cggcccgcaa acttcctggc gcgaacggtt
     3241 cgcaggcctg aaaaatatct gggcgccggt gctcttgttc gtgttcgtca ttggcgggct
     3301 ctacgggctg ccattcctgc cgcgcttcac cccgaccgag gcgggcggcg tcggcgcgac
     3361 cggcgcattc ctgatcggcc tgttcaccgg acggttggac aaagagaaga ttctggcctc
     3421 gctgctgcag gcgacccgca ccgccgccgc cgtctttacg gtgctgatcg gagccttgat
     3481 ctttggatat ttcctcacgg tgacgcagac cccgcagaag gtcacggagt ttctcaccgg
     3541 cctcggtctc ggcagctacg gcgtgctcgc cctcatcatg gtgatgtatc tggtgctcgg
     3601 ctgcctgatg gacgcgatgg cgatgatcat tctgaccgtg ccgatcatct ttccggtgat
     3661 cacccatctc ggctttgatc cgatctggtt cggcgtcatc atcgtgatga cggtggaact
     3721 cggcctgatc catccaccgg tcgggatgaa cgtcttcgtg atcaagagcg tcgtcaagga
     3781 cgtgtcgttt gccacgattt tcaggggcgt gatcccgttc gtggcgaccg acctgatccg
     3841 gctggtgatc ttgatcctgt ttccattgct ggcgacctgg ctgccgcagc gcatgatggg
     3901 atgagcatga tccggaaaag catgccctcg ggccgcgacc cgatgggtgg acaccggttt
     3961 tccgacaaga tcatgctcaa atcaaaagag agcatggtaa cgccgcagct ttccaccaga
     4021 tatgttttca ccattaccgc caatatcggc agcgtgacat ccgccggcga tgtcggtcac
     4081 ggcgtccgcc gcatcattcc gattaccggt ggcgacgtgc gcggcgatat caacggcaag
     4141 gtctgcgcgt tcggcgcgga cttccagatc atccggccca atgagctgat cgaactggaa
     4201 gcgaaatatg ccttcgagac cgacgacggc gcggt
//
LOCUS       DASWGM010000159         4468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_148754,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000159 DASWGM010000000
VERSION     DASWGM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148754"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VGA15_04825"
     CDS             <1..369
                     /locus_tag="VGA15_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9587049.1"
                     /translation="PKTPQDTKLIPISRTIGAHRVVDEMLFCFTHDIEIDWMLPGVAP
                     TGKYVEIPLVAIVRFRGDKLYNEHIYWDQASVLVQIGLLDPALLPVAGVATAKKLVDE
                     TLPSNTLMARWAESAPQAAE"
     gene            429..1967
                     /locus_tag="VGA15_04830"
     CDS             429..1967
                     /locus_tag="VGA15_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587050.1"
                     /translation="MSPALSRRTVLQLAGAAALPLSSGLFDAAQAAPSGTLTIAYNVN
                     LPSFDPTVGLSAVNPTIQSIYQSIFDTYVGQAPDLSFKPGLLTKWGWNADRTKITLEL
                     RKGATWHDGSPVTPEDVVWSLRRAADTKSGNPIQFVWSKVGNFKIDGNTITGDVLEFE
                     PTLFKWMAFLTAFVLPKAYYEKVGAEGFEKTPVGSGPYKVDAFERNAFLRLKAHPGYW
                     GAKPAFDTVVFKFVTDPTSRVAEIESGGSDVTFEIPYEEFDRLKAKPGLAGQTKPVSD
                     IAMIFITDIDPMLDRNVRLAANHAIDKKAIVDRLLKGYGVPIATLEAPGYAAFDPTIK
                     VTYDPDLAKSLLAKSGFSPEKPVKFTIQTTRGFKPKDYEMIQAIVGMWRKVGIEATIE
                     VYEIAQHFELRARHALAPAAFYNWGNAIGDPSTSTGFAMFGPSPHCAWKGKDLVERIG
                     PLWGEKDEAKRIAGWKAVDKYIAEEGEVIPLFQYVQPIIHKKGLKIVAQANGMILPQL
                     ITQA"
     gene            1994..3001
                     /locus_tag="VGA15_04835"
     CDS             1994..3001
                     /locus_tag="VGA15_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9587051.1"
                     /translation="MRWGEIIRRLVLIPPTLVGVAVIVFVLLRVVPGDPIAMMIPPGA
                     SPADVDRLRAFYGLDQSIPQQFITWSGQALSGHFGRSISLHQQVFELVLARLPATLEL
                     ALLATLIAMVLGLIAALIGTQVRGRRAEWLVDGGVGILLSIPDFLWALILLLLFGVLV
                     PLLPISGRIDPEIDVSFRSNFYLIESLLTARFDVASALLHHMILPALALALPFAALLA
                     RVLKTSLAEAEDQDYAQVARARGFSRPKILLREVFPNALIPTVALGGVQVTLLLGGTV
                     LVERIFSYEGIGNMAIDAVINRDFPLIQGLILTFAALFIAINLFVDLLVTLLDPRLRH
                     G"
     gene            2994..3881
                     /locus_tag="VGA15_04840"
     CDS             2994..3881
                     /locus_tag="VGA15_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9587052.1"
                     /translation="MAELAPERPVQRPSRWIAALRRGGPRLWIGATLVGVLVLVALLA
                     PLLAPHNPIEQDLMSAQLPPAWSEGGEPAYLFGTDSLGRCVLSRLIYSARTAVAVALI
                     AASLAAAIGIAFGLIAGSFGGWVDQLTSRLIDVWMAFPPVLLSIVLAAVIGAGLTSVI
                     TAIVVVDWTRFARVVRAETLVQLQRDYAAAARVIGLSRGAILRLEILPNLVPLVVTLL
                     SVEMGIAILVEVILSFVGISVTGDTPTWGGMIAEGRQIVYQAPWIMALPIGCIISSVI
                     GFNLLGDGLRTVLDPVQRR"
     gene            3878..>4468
                     /locus_tag="VGA15_04845"
     CDS             3878..>4468
                     /locus_tag="VGA15_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587053.1"
                     /translation="MTTTLSVQDLHVAIGKGAHSANVLRCVSLELRPGEVRGLVGESG
                     AGKSMLGRAVLGLLPANATIAAGTISFEGRDLIAMPEPERRGLFGRRIALIPQDPMTS
                     LNPVKRVGAQIAILLRHHLGLSKRAALARAAELLSEVAIRDPGRVLGLYPHEISGGMR
                     QRVLIAMAFACDPSLVIADEPTTALDVTVQRQVLQLV"
BASE COUNT          722 a         1435 c         1437 g          874 t
ORIGIN      
        1 ccgaagacgc cgcaggacac caagctgatc ccgatctcgc gcaccatcgg cgcgcatcgc
       61 gtggtcgacg agatgctgtt ctgcttcacc cacgatatcg agatcgactg gatgctgccc
      121 ggcgtggcgc cgaccggcaa atatgtggaa attccgctgg tcgcgatcgt ccgcttccgc
      181 ggcgacaagc tctacaacga acacatctac tgggatcagg cttcggtgct ggtgcagatc
      241 ggactgctcg atccggcgtt gcttcccgtg gccggcgtgg cgacggcgaa gaaactcgtc
      301 gatgagaccc tgccgtcgaa tacgctgatg gcgcgctggg ccgagagcgc gccgcaggcc
      361 gccgaataat cgcaatctgt taacggcgcc cgctcccggg cgctgttctc gcatatggag
      421 taatggtcat gtcccccgcg ctttctcgtc gtaccgtgct gcagctggcc ggtgccgccg
      481 cgttgccgtt gtcctccggt ctgttcgacg cggcgcaagc agcaccctcg ggaacgctga
      541 ccatcgccta taacgtcaat ttgccgtcgt tcgatccgac cgtcggattg tccgcggtca
      601 atccgaccat ccagtcgatc tatcagtcga tcttcgatac ctatgtcggt caggcgcctg
      661 atctgtcctt caagcccggc ctgctgacca aatggggctg gaacgccgac cgcaccaaga
      721 tcacgctgga gctgcgcaag ggggcgacct ggcacgacgg atcgccggtg acgcctgaag
      781 acgtggtctg gtcgctgcgg cgcgccgccg acaccaaaag tggcaatccg atccagttcg
      841 tctggtccaa ggtcggcaac ttcaagatcg acggcaatac catcaccggc gacgtgctgg
      901 aattcgaacc gaccttgttc aagtggatgg ccttcctcac cgcctttgtt ctgccgaagg
      961 cctattatga gaaggttggc gcggagggtt tcgagaaaac gccggtcggc tccggtcctt
     1021 acaaggtcga cgccttcgag cgcaacgcgt tcctgcggct caaggctcac cccggttatt
     1081 ggggcgccaa gcccgcgttc gataccgtgg tattcaaatt cgtcaccgat cccaccagcc
     1141 gcgtcgccga aatcgagtcc ggcgggtcgg acgtcacgtt cgaaattccc tacgaggaat
     1201 tcgaccgcct gaaggcgaag ccgggcctcg ccgggcagac caagccggtg tccgacatcg
     1261 cgatgatctt catcaccgac atcgatccga tgctcgaccg caatgtccgc ctcgccgcca
     1321 atcacgcgat cgacaagaag gcgatcgtcg atcggttgct gaaggggtat ggcgttccga
     1381 tcgcgacgct ggaagcgccg ggctatgccg ccttcgatcc cacgatcaag gtaacttacg
     1441 atcccgacct cgccaaaagc ctgctggcca agagcggctt ctcgccggag aagccggtca
     1501 agttcaccat ccagaccacg cgcggcttca agccgaagga ttacgagatg atccaggcga
     1561 tcgtcgggat gtggcgcaag gtcgggatcg aggccaccat cgaggtctac gagatcgcgc
     1621 agcatttcga gctgcgcgcg cgccacgcgc tggccccggc cgcgttctac aattggggca
     1681 acgcgatcgg cgatccctcg acttccaccg gctttgcgat gttcggcccg tcgccgcatt
     1741 gcgcctggaa gggcaaggat ctggtggagc gcatcggccc gctgtggggt gaaaaggacg
     1801 aggccaagcg gatcgccggc tggaaggccg tcgacaaata catcgcggaa gaaggcgagg
     1861 taatcccgct gttccagtat gtgcagccga tcatccacaa aaagggactg aagatcgtgg
     1921 cgcaggccaa cggcatgatc cttccgcaac tgatcacaca ggcttaagga ctgtaaagca
     1981 gggcgacacg acgatgcgat ggggcgagat catcaggcga ctcgtcctca taccgcccac
     2041 gctggttggc gtggcggtga tcgtctttgt gctgctgcgc gtagtgcccg gcgatccgat
     2101 cgccatgatg ataccgccgg gcgcctcgcc agccgacgtc gatcggctgc gcgcgttcta
     2161 tgggctcgac caatcgattc cacagcagtt catcacctgg tccggccagg cgctgagcgg
     2221 ccatttcgga cggtcgatca gcctgcacca acaggtcttc gagctggtgc tggcgcgatt
     2281 gccggcgacg ctcgaactgg cgctgctggc gacgctgatc gcgatggtgc tgggtctgat
     2341 cgcggcgctg atcggcaccc aggttcgcgg ccggcgggcg gaatggctgg tcgacggcgg
     2401 cgtcggcatc ctgctttcga ttccggattt tctctgggcc ctgatcctgc tgttgctgtt
     2461 cggcgtgctt gtcccgctgc tgccgatttc gggccgcatc gatccggaga tcgacgtcag
     2521 cttccgcagc aatttctatc tgatcgaaag cctgctgacg gcgcgcttcg acgtcgccag
     2581 cgcgctgctg catcacatga tcctgccggc gctggcgctg gcgctgccat tcgcggcctt
     2641 gctggcgcgg gtcttgaaaa cctcgctggc ggaggccgag gatcaggatt atgcgcaagt
     2701 agcccgcgcc cgcggctttt cacgccccaa aatcctgctg cgcgaagtgt ttcccaatgc
     2761 gctgatcccc actgtcgcgc tcggcggcgt ccaggtcacg ctgctgctcg gcggcaccgt
     2821 gctggtcgag cgcatcttct cctatgaggg aatcggcaac atggcgatcg acgcggtgat
     2881 caatcgcgat tttcccctga tccagggctt gatcctgacc ttcgccgcgc tcttcatcgc
     2941 gatcaacctc ttcgtcgatc tcctggtcac gctgctcgat ccgaggttgc gccatggctg
     3001 agcttgcccc cgagcgtcca gtgcagcgtc cgtcgcgatg gatcgccgcg ctccggcgcg
     3061 gcggtccgag gctctggatc ggcgcgacgc tggtgggcgt cctcgtgctg gtggcgttgc
     3121 tggcgccgct gctcgcacct cacaatccga tcgaacagga tctgatgtcg gcgcaattgc
     3181 cgcccgcctg gtccgaaggc ggcgagccgg cctacctgtt cggcaccgac agtcttggcc
     3241 gttgcgtgct gtcgcgactg atttactcgg cgcggaccgc ggttgccgtc gcgctgatcg
     3301 cggcttcgct ggcggcggcg atcgggatcg ccttcggcct gatcgcgggc agtttcggtg
     3361 gctgggtcga tcagctcacc tcgcgcctga tcgatgtctg gatggcgttt ccgccggtgt
     3421 tgctgtcgat cgtgctggcg gcggtgatcg gcgcggggct gacctcggtg atcacagcca
     3481 tcgttgtcgt cgactggacc cgctttgcgc gcgtggtccg ggccgaaact ctggtgcagc
     3541 tgcagcgcga ttatgccgcc gccgcgcgcg tcatcggatt gagccgcgga gcaatcctgc
     3601 gcctggagat tctgcccaat ctcgtgccgc tggtggtcac cctgctgtcg gtcgagatgg
     3661 gcatcgcgat cctggtggaa gtcatcctgt cttttgtcgg catttcggtg accggcgaca
     3721 ccccgacctg gggcggcatg atcgccgagg gacgccagat cgtctatcag gcgccatgga
     3781 tcatggcgct gccgatcggc tgcatcatat caagcgtgat cgggttcaat ctgctcggcg
     3841 acggcctgcg cacggtgctc gatccggtgc agcgccgatg acaacaaccc tttccgtcca
     3901 ggacctgcac gtcgcgatcg gcaagggagc acattcggcc aatgtgctgc gctgcgtcag
     3961 tctcgagcta aggcctggcg aggtccgcgg gctggtcggc gaatccggcg ccggcaagtc
     4021 gatgctggga cgcgccgtgc ttgggctgtt gcccgccaac gcgaccatcg ccgccggcac
     4081 gatctcattc gagggacgcg atctgatcgc gatgccggag ccggagcgac gcggtctgtt
     4141 cggccgccgc atcgcgctga ttccgcagga cccgatgacc tcgctcaatc cggtgaagcg
     4201 cgtcggcgcg cagattgcga ttctgctgcg gcatcacctt ggcctgtcga agcgcgccgc
     4261 gctggcgcgc gccgctgagc tgttgtcaga ggtcgcgatc cgcgacccgg ggcgggtact
     4321 cgggctttac ccgcatgaga tttccggcgg gatgcggcag cgcgtgctga tcgcgatggc
     4381 gttcgcctgc gatccgagcc tggtgatcgc ggacgagccg accaccgcgc tggatgtcac
     4441 cgtgcagcgt caggtgctgc agctggtc
//
LOCUS       DASWGM010000160         5346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2508614,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000160 DASWGM010000000
VERSION     DASWGM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2508614"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="VGA15_04850"
     CDS             <1..1442
                     /locus_tag="VGA15_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX9587054.1"
                     /translation="ALAIMLGANVGTTLIVQILSFNIAAIAPVLFIIGLVAFRSGARS
                     RIKDIGRVSIGLGLMLLALHILLDTLAPAENAPGMRVFMSAITADPVLCIVIGAIVTW
                     AVHSSVASVLLVMSLAYAQFISPDASLALVLGANLGSAINPVLEGARRDNPASYRLPV
                     GNLINRLIGVLLVAPFLHPITETLRAWQPDLAKLTAEFHIAFNVATAIIFIGLLDGMA
                     RLLKRLFPNRIQETDPSRPRYLDESALETPSLALADAARETLHMGDLVEVMLRKVMAA
                     MMTNDRALVDQVSRMDNAVDSLDEAIKLYVTKLTRGSLDEREGQRAMEIISFAINLEH
                     MGDIIDKNLSELATKKIKRRFQFSPEGAEELSAFHKRTMDSLRIAFGVFMSGDVNEAR
                     KLLAEKALLRNTELAATERHLDRLREGRPETIETTSLHLDVLRDLRRIHSHICSVAYP
                     VLDAAGELAVYRSTESDVAALPAAHPLPR"
     gene            complement(1588..1917)
                     /locus_tag="VGA15_04855"
     CDS             complement(1588..1917)
                     /locus_tag="VGA15_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9587055.1"
                     /translation="MTDLLNTFVTYLHDVFVIKFDGWVILGFVAQAFFTMRFVVQWIA
                     SERARKSVIPVAFWFFSIGGGALLLVYALYRRDPVFIAGQALGLVVYVRNLYFIIVNG
                     RQASADG"
     gene            complement(1910..2662)
                     /locus_tag="VGA15_04860"
     CDS             complement(1910..2662)
                     /locus_tag="VGA15_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9587056.1"
                     /translation="MPSSDAATVDVSIVVPVRNEAENVAPLIAEIVSALGGRWSYEII
                     YVNDGSTDATAERLAAIMKQRENLRQIRHATSSGQSAAVRSGVRAARGAIVATLDGDG
                     QNNPAFLPDLISAIEKGGERVGLAAGQRVGRKDTGFKKIQSRIANGVRNGILRDGTRD
                     TGCGLKAFRREVFLMLPYFDGLHRFLPALVRREGYDIAYVDVTDRPRRSGVSNYGFFD
                     RLWIGIMDLAGVWWLIRRKKPTPVATEVISND"
     gene            complement(2853..3701)
                     /locus_tag="VGA15_04865"
     CDS             complement(2853..3701)
                     /locus_tag="VGA15_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9587057.1"
                     /translation="MSVQAGIGGQSSYFSQVLFLARMSATQLVRPPSHSRRAEAARRS
                     ARRGLLLVAIVSAVIVALMYALDVTEIGLMPPRGTASLWPVRILTDFGKSAYVLWLLA
                     IMLFAVAIMSPRLRGTSRSLLLSFGMRLQFLFFAVLVPVLTGEVIKWIVGRGRPFAGG
                     KADAFNFAPFAGTEAYASFPSGHAIASFALAFAVSAVWPRARAAMMVYAILIAASRLV
                     LLAHHPSDVVAGALIGIVGAMFVRYWFAARRLGFAIRRDGTIVPLRSIFRLSGIRLAE
                     ENAPDK"
     gene            complement(3702..>5346)
                     /locus_tag="VGA15_04870"
     CDS             complement(3702..>5346)
                     /locus_tag="VGA15_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9587058.1"
                     /translation="TASHVRAIAFLLLCGLLLFLPGFFNIPPIDRDEARFAQATKQMV
                     ESGDFVDIRFQDDVRYKKPVGVYWLQAAAVETASALGLPRAQVRVWLYRVPSLIGAIG
                     AVLLTYWTALAFVTRRGAVLAGLILGSSVLLGVEARLAKTDAMLLLTVVAAMGALARV
                     YLSWQRGEDPAHPPWTWPAIFWTALAAGILLKGPLILMFVGLTIATLAILDRSATWLW
                     RLRPVWGLMWLLVLVLPWFIAIFWRAGDAFFTDSIGGDMLSKLAAQESHGAPPGVYLL
                     LFWVTFWPGAPLAAMATPAVWRARREPGAQFLLAWLVPSWIVFELVLTKLPHYVLPLY
                     PAIAILTIGALERRVLSRSWLMRGAAWWFIIPAGASVIAVIGAITLTRQPVFLAWPFV
                     GAALIFGLFAWWLYDDNRAERSLLNAVVAAMFLAVAIYGVIVPALTPLFPSSEIARAL
                     RNVVCVGPKAAAAGFHEPSLVFMTGTSTVLTDGSGAADFLGQGSCRFALVEARSERGF
                     AQRAEAIGLRYNVAARIDGYNISQGRAISIAIFRSEGTE"
BASE COUNT         1097 a         1776 c         1611 g          862 t
ORIGIN      
        1 cggcccttgc catcatgctc ggcgccaacg tcgggaccac gctgatcgtt caaatcctgt
       61 ccttcaatat cgccgccatc gcgccggttc tctttatcat cggcctggtc gctttccgca
      121 gcggtgcgcg ctcgcgcatc aaggacatcg gccgtgtctc gatcgggctc ggcttgatgc
      181 tgctggcgct gcatattctg ctcgacacgc tggcgccggc ggagaatgcg cccggcatgc
      241 gtgtgttcat gagcgccatc accgccgatc ccgtgctctg catcgtgatc ggcgcgatcg
      301 tcacctgggc ggtgcattcg agcgtcgcca gcgttctcct ggtcatgtcg ctggcttacg
      361 cccaattcat ttcgccggat gcatctttgg cgcttgtgct cggcgccaat ctcggcagcg
      421 cgatcaaccc ggttctcgaa ggcgcgcgcc gcgacaatcc cgccagctac cgtcttcccg
      481 tcggcaatct catcaatcgg ctgatcggcg tgctgctggt tgcgccgttc ctgcacccga
      541 tcacggaaac tttgcgggcc tggcaacccg acctcgccaa gctcacggcc gaatttcata
      601 tcgcgttcaa cgtggcgacc gcgatcatct tcatcggctt gcttgacggc atggcccggc
      661 tgctgaaaag gctttttccg aaccggatcc aggaaaccga tccgtcgcgg ccgcgctatc
      721 tcgacgagag cgcgctggaa accccgtcgc tggctttggc ggacgcggcg cgtgaaaccc
      781 tgcacatggg cgatctcgtc gaggtcatgc tgcgcaaggt gatggctgcg atgatgacca
      841 acgatcgcgc tctggtcgac caggtgtcgc ggatggacaa tgccgtcgac agtctcgacg
      901 aggcgatcaa gctttatgtg accaaactga cgcgcggcag cctcgacgag cgcgaaggac
      961 agcgggcgat ggagatcatc tcctttgcca tcaatctcga gcatatgggc gacatcatcg
     1021 acaagaattt gagcgagctc gcgaccaaga agatcaagcg caggttccag ttttcgcccg
     1081 agggcgcgga agaactgtcg gcattccaca agcgaaccat ggactcgctg cggatcgctt
     1141 tcggcgtctt catgtcgggc gacgtcaatg aagcgcgcaa gcttctcgcc gagaaggcgc
     1201 tgttgcgcaa taccgagctt gccgccaccg aacggcatct ggaccgtctg cgcgaaggcc
     1261 ggccggagac gatcgagacc acctcgctgc atctcgacgt gctgcgtgac ttgcggcgca
     1321 tccactcaca tatctgctcg gtggcttatc cggtcctcga cgcggccggc gaactggccg
     1381 tctatcgaag caccgaaagc gatgtggcgg cgcttcctgc tgcgcatccc ctgccgcgat
     1441 aatttatggc actcgctggc cccggccgtc atcctgaggc gcgagccgtc ttcggcgagc
     1501 ctcgaacgat ggcagcgaaa cgcgtagccc atccttcgag gcgcgcacga gcgcgcacct
     1561 caggatgacg gcaatgcacg cgcaacgtca accatccgcc gaggcctgcc gtccgttcac
     1621 gatgataaaa tacagattgc ggacgtagac cacgagcccg agcgcctggc ctgcgatgaa
     1681 taccgggtct ctccgataca gcgcatagac cagcagcagc gcgccaccgc caatcgagaa
     1741 gaaccagaac gccaccggga tcacgctctt gcgcgcgcgc tctgacgcga tccattgcac
     1801 cacaaagcgc atggtgaaaa atgcctgcgc cacgaacccc agaatgaccc agccatcgaa
     1861 tttgatgacg aagacatcgt gcaggtaggt cacgaatgtg ttgagcaagt cagtcattgg
     1921 atattacctc agtcgcaacc ggcgtcggct tcttgcggcg gatcagccac cacacgccgg
     1981 cgagatccat gatcccgatc catagccgat cgaaaaaacc gtaatttgac acgccggagc
     2041 ggcgcggccg atcggtcacg tcgacatagg cgatgtcgta gccttcgcgg cgaaccagcg
     2101 ccggcagaaa acgatgcagc ccatcgaagt aaggcagcat caggaacacc tcgcgccgga
     2161 acgccttcag gccgcagccg gtatcgcggg tgccatcgcg caggattccg ttgcgcacgc
     2221 cattggcgat gcgcgactgg attttcttga aaccggtgtc cttgcgcccg acgcgctggc
     2281 cggcggcaag accgacgcgt tcgccgccct tctcgatcgc cgaaatcaag tcgggcagga
     2341 atgccggatt attttgtccg tcgccgtcga gggtcgcgac aatggcgcca cgcgccgcgc
     2401 gaaccccgct gcgcaccgcc gccgactggc ctgatgacgt ggcgtgacgg atctgccgca
     2461 gattttcgcg ttgcttcatg atcgcggcaa gccgttcggc ggtcgcatcg gtcgagccgt
     2521 cattgacgta gatgatttca taactccatc gaccgccgag ggcagagacg atctcggcga
     2581 tcagcggcgc tacgttctcg gcctcgttgc gcacggggac aacgatggaa acatcgaccg
     2641 tggcggcgtc agaagatggc aaatcaacac tcgtcgcttg agcagggctg gcttgagttg
     2701 ggccgccgcc ggtgacgctt gcgtccggcg cttgcggcgt ccgcttctta tggggcgaaa
     2761 gcgtcgcggg caaccctttt gaggtgccct ggcgagggtc agcgcgtatt ccgtaaaagt
     2821 ggataccggt tttgcgaata gaatacgcgc aattatttat caggagcatt ttcttcggct
     2881 aaccggattc cgcttagccg gaaaatgctc ctgaggggca caatcgtgcc gtcgcggcgg
     2941 atcgcaaacc cgaggcggcg cgcggcgaac cagtaccgca cgaacatcgc cccgacgatg
     3001 ccgatcaacg cgcccgcgac cacgtcgctc ggatgatgcg ccagcagcac gaggcggctg
     3061 gcggcgatca gaatggcata caccatcatc gcggcccgtg cccgcggcca aaccgctgat
     3121 acagcgaacg ccaacgcaaa gctcgcgatg gcatgccccg atggaaagct ggcataggct
     3181 tcggtgccgg cgaagggggc gaaattgaag gcatcggctt tgccgccggc aaacggccgg
     3241 ccgcgcccga cgatccattt gatgacctcg cctgtgagca ccggcaccag cacggcaaaa
     3301 aacagaaact gcagacgcat gccaaaactg agcagcagcg atcgcgaagt cccccgcaat
     3361 cgcggcgaca tgattgcgac ggcgaacaac atgattgcca gcaaccacag cacataggcc
     3421 gattttccaa agtcggtcag gatgcgaacg ggccaaaggc tggcggtccc ccgcggcggc
     3481 atcaggccga tttcggtaac gtcgagcgca tacatcagcg cgacaatgac ggcactcacg
     3541 atagcgacca gcagcaaccc gcggcgcgcc gatcttcgtg ccgcctcggc gcgccgcgaa
     3601 tgcgaaggcg gccgcaccag ttgcgtcgcc gacatcctcg ccaggaagag aacctgcgaa
     3661 aaataactcg actgtccgcc gatgccggcc tgaacggaca tctactccgt gccctcggag
     3721 cggaagatgg cgatggaaat cgcccgacct tgcgaaatat tatagccgtc gatgcgggcg
     3781 gcgacgttgt agcgcagccc gatggcttcg gcgcgctggg caaagccgcg ctccgaacgc
     3841 gcttcaacca gcgcgaatct gcaactgccc tgccccagaa agtccgcggc gcccgatcca
     3901 tccgtcagca ccgtcgaggt gccggtcatg aagacgagac ttggctcgtg aaaaccggcc
     3961 gcagcggcct tcggtccgac gcagacgacg ttgcgcagcg cgcgcgcgat ttcagaactc
     4021 gggaatagcg gcgtcagcgc cggcacaatg acgccgtaga tcgccacggc caaaaacatc
     4081 gccgccacca cggcgttgag cagcgagcgc tcggcgcgat tgtcgtcata aagccaccac
     4141 gcgaacagcc cgaagatgag cgctgcgccc acgaacggcc acgccagaaa taccggctgc
     4201 cgcgtcagcg taatcgcgcc gatcacggca atgaccgaag ccccggcggg aatgatgaac
     4261 caccaggcgg cgccgcgcat caaccatgat cgcgacagca cgcggcgctc cagcgcgccg
     4321 atggtcagga tcgcgatcgc cggatacagc ggcagcacat aatgaggcag cttggtcagc
     4381 accagttcga acacgatcca ggaaggaacc agccacgcca gcagaaactg cgcgccgggc
     4441 tcccgcctcg cccgccacac cgcgggcgtt gccatcgccg ccagcggcgc tcccggccag
     4501 aacgtcaccc agaacagcag cagatagact cccggcggcg cgccatgaga ttcctgcgcg
     4561 gcgagcttgc tcaacatgtc gccgccgatc gaatctgtga aaaacgcgtc accggcgcgc
     4621 cagaagattg cgatgaacca gggcaacacc agaaccagca gccacatcag gccccatacc
     4681 ggccgcagcc gccacagcca tgtcgccgag cgatcgagga tcgccagcgt ggcgatcgtc
     4741 agcccgacga acatcagaat caacggtccc ttgagcagga tgccggcggc cagcgccgtc
     4801 cagaaaatcg ccggccatgt ccacggcgga tgcgcgggat cctcgccccg ttgccatgac
     4861 aaatacaccc gcgccagcgc ccccatcgct gcgacgacgg tgagcagcag catcgcatcg
     4921 gttttcgcca gccgcgcttc gacgccgagc agcaccgaac tgcccaggat cagcccggcc
     4981 aggacggctc cgcgccgtgt gacgaatgcc agcgccgtcc agtaggtcaa cagcacggcg
     5041 ccgatcgcac cgatcaggga cggcacacgg tacagccaga cgcgcacctg cgcccgcggc
     5101 aagcccagcg ctgacgcggt ttcgaccgcc gctgcctgca gccagtacac gccgaccggt
     5161 ttcttgtagc gcacgtcgtc ctggaaacgg atgtcgacga agtccccgct ctcgaccatt
     5221 tgcttggtcg cctgcgcgaa ccgcgcctcg tcgcggtcga tcggcggaat gttgaaaaat
     5281 ccgggcagga acagcagcag accgcacagc agcaggaacg caatcgcgcg gacatgactg
     5341 gcagtg
//
LOCUS       DASWGM010000161         3817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2092375,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000161 DASWGM010000000
VERSION     DASWGM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092375"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VGA15_04875"
     CDS             complement(<1..284)
                     /locus_tag="VGA15_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX9587059.1"
                     /translation="MMKIDKTHLPLIVPNPGDPRLTERVAGTDQTGAAVEIRVPVERP
                     LTLYLNAQEIVTMMTIGDYPEYLALGYLLNQNMLKYDDVVTEVEYDDDLQV"
     gene            377..904
                     /gene="mobB"
                     /locus_tag="VGA15_04880"
     CDS             377..904
                     /gene="mobB"
                     /locus_tag="VGA15_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX9587060.1"
                     /translation="MNVIGLAGWSGAGKTTLLTRVIPHLLGQGLRVSVIKHAHHSFDV
                     DVPGKDSWVHRQSGAAEVLVSSGQRWALMHELRGASEPRLPELLAKLSPVDLVIVEGF
                     KAEPHRKIEVHRLANGKAVLFPSDPGIVGIATDADLETALPVAHLDDIPAIASMMRRS
                     AISVEDLLARSAPGT"
     gene            914..2167
                     /gene="glp"
                     /locus_tag="VGA15_04885"
     CDS             914..2167
                     /gene="glp"
                     /locus_tag="VGA15_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX9587061.1"
                     /translation="MAQLSDDCFAFGGPMMSVDEAVAIIAARVSPVQDIEAVLLAAAD
                     GRVLAKDISAPLPLPPFTNSAVDGYAVRSGDLPQAAEKAFPVVGRVQAGASAQAPVEA
                     AHAVRIFTGAPMPRGADTVFMQEDVRLDQGSVVLPAGLKPGANVRPAGEDVPAGHCAL
                     KSGRRLRPQDVALVAAFGLTHVEVRRRIRVAVFSTGNELVSPGVPRGAAQLFDSNRFM
                     LMAMLVRLGCEVSDLGILGDDRMALASALQNVADAHDLVLTTGGVSTGEEDHVKTAVE
                     SVGNLVLWRMAIKPGRPVAMGIIGGTPFIGLPGNPVASFVTFVHVVRPTVLALAGAAQ
                     QPLVPMPVRAAFTYKKKIARREYVRVNLRKADDGVLEVAKFPREGAGLLSSLVDTDGL
                     VELGEDITRVEPGQTVGFLSYANLI"
     gene            2229..2498
                     /locus_tag="VGA15_04890"
     CDS             2229..2498
                     /locus_tag="VGA15_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX9587062.1"
                     /translation="MIRTKLDLTGLKCPLPALKTRKALKTLTPGDLLEVHCTDPLSVI
                     DIPNLIRETGDKIEITERVEHEIVFLIEKAGGSSPAESSVRHQDS"
     gene            2888..>3817
                     /locus_tag="VGA15_04895"
     CDS             2888..>3817
                     /locus_tag="VGA15_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587063.1"
                     /translation="MTTISSDGTMSGAKKEGILDREHTIAKAGFNRWLVPPAALCIHL
                     CIGMAYGFSVFWLPLSRAVGLTAPKACPDMSLLQELFTTTCDWRVASMGWMFTLFFVL
                     LGVSAAIWGGWLERVGPRKAGVVAALCWGGGLLIGAFGVYVHQLWIMWLGAGVIGGVG
                     LGLGYISPVSTLIKWFPDHRGMATGFAIAGFGGGAMIGAPLANLLITYFKTPTSVGVW
                     ETFATMGVVYFVFMMIGAFAYRVSPVGWQPEGWTAPAKANAMISANNVHLNDAHKTPQ
                     FWLIWGVLCLNVSAGIGVIGMASPMLQEIFAGKL"
BASE COUNT          658 a         1145 c         1238 g          776 t
ORIGIN      
        1 acctgcagat cgtcgtcata ctcgacctcg gtgacgacat cgtcgtattt cagcatgttc
       61 tgattgagca gatagccgag cgccagatat tccggatagt cgccgatcgt catcatggtg
      121 acgatctcct gcgcgttcag atacagcgtc agcggccgtt ccaccgggac cctgatctcg
      181 accgcggccc cggtctggtc ggtgccggcg acgcgttcgg tcagccgcgg atcgccagga
      241 tttggcacga tcagcgggag gtgagttttg tcgatcttca tcataggcga caggctagca
      301 tgacatggcc gatcaaccga tataagcagc tttggacaca gcatagcccg gttccgccgg
      361 cagcccggag aatatgatga acgtgatcgg cctggcggga tggagcggcg ccggcaagac
      421 gacgctgttg acgcgggtca ttccccatct ccttggccag ggtcttcgcg tttccgtcat
      481 caagcacgcc caccacagtt tcgatgtgga tgtaccgggc aaggattcct gggtgcaccg
      541 gcagtcgggc gccgccgaag tgctggtttc ttccggccag cgatgggcct tgatgcatga
      601 attgcgcggg gcctcggagc cgaggctgcc ggaattgctg gccaagctgt cgccggtcga
      661 tctggtgatc gtcgaagggt tcaaggccga gccgcatcgc aagatcgagg tgcatcgcct
      721 cgccaatggc aaggcggtgc tgttcccaag cgatcccggt atcgtcggca tcgccaccga
      781 cgcggatctg gaaacggcgc tgccggtcgc ccatctcgat gacatcccgg cgatcgcttc
      841 tatgatgcgg cggtccgcga tatctgtcga agacctgctg gcgcgaagcg cgcccggaac
      901 ctgagcagcc aaaatggcgc aattgtccga cgattgcttt gcgttcggcg ggccgatgat
      961 gtcggtcgat gaggccgtcg cgatcatcgc cgcgcgggtc agcccggtac aggacatcga
     1021 ggctgtcttg ctggccgcgg ccgatgggcg cgttctcgcc aaggacattt ccgcgccgtt
     1081 gccgctgccg ccgttcacca attccgccgt cgacggctat gcggtgcgca gcggcgatct
     1141 gccgcaggcg gcagaaaagg catttcccgt cgtgggtcgc gttcaggccg gcgcgtcggc
     1201 gcaagcaccg gtcgaggcgg cccatgcggt gcggattttt accggggcgc cgatgccgcg
     1261 aggtgccgac acggtcttca tgcaggaaga tgtgcgcctc gatcagggca gcgtcgttct
     1321 tcccgcgggc ttaaaacccg gcgccaatgt gcgccccgcg ggcgaggacg ttcccgccgg
     1381 tcactgcgcg ctcaaatcag gccggcgctt gcgcccgcag gatgtcgcgc tcgtggccgc
     1441 gttcggcctg acgcatgtcg aggtgcgccg gcgcatccgt gtcgcggtgt tttcgaccgg
     1501 caacgagctg gtatcgccgg gggtgccgcg cggggccgcg caattgttcg actccaatcg
     1561 cttcatgctg atggccatgc tggtgcggct tggctgcgag gtcagcgatc tcggtatcct
     1621 cggagacgat cgcatggcgc tcgcgagcgc gttgcagaac gtggcggatg ctcacgacct
     1681 cgttctcact accggtggcg tttcgaccgg cgaagaggac cacgtcaaga ctgccgtgga
     1741 aagcgtcggg aacctggtgc tgtggcgcat ggcgatcaag cccggacgtc cggtcgccat
     1801 gggaattatc ggcggcacgc cgttcatcgg attgccgggc aatccggtgg cgagttttgt
     1861 cacttttgta cacgtggtgc ggccgaccgt gctggctctc gcgggcgccg cacaacagcc
     1921 tctggttccg atgccggtcc gcgccgcctt cacctacaag aaaaaaatcg cccgccgcga
     1981 atatgtccgg gtcaatctgc gcaaggccga tgacggcgtg ctcgaagtgg caaaatttcc
     2041 ccgcgagggc gctggactgc tgtcatcgct ggtggatacc gacggccttg tcgaacttgg
     2101 cgaggacatt acgcgggtcg aaccgggcca gacggttgga tttctcagct atgcaaatct
     2161 gatctgacgc tcgttgtttc gggcgatgtg cgttgacgat gtgacaccct ttcgccatgt
     2221 atgggcgcat gatcaggacc aaactcgacc tcaccggtct caaatgtcca ttgccggcat
     2281 tgaagacacg caaggcgttg aaaacgctta cacccggcga cctcctggaa gttcactgca
     2341 ccgatccgtt gtcggtcatc gatatcccca atctgattcg ggaaaccggc gataagatcg
     2401 agatcacgga acgtgtagag cacgaaatcg tgtttctgat tgaaaaggct ggcggttcgt
     2461 cgcctgctga gtcgtcagtc cgacatcagg acagttgaac gcgcgtcaat gtgtcctgtt
     2521 gcatcccgaa tagttgataa cagggtagac cgaacagttg atggcagggt agagcgctac
     2581 cttttgccta tcgacacagc gtgcggcttc tgccccaatt gacttctccg caggggtgcg
     2641 gaggatgggt cgcggctgtg tcgcggcaca cgctgagcgg gcggacatat tctcaactca
     2701 agaccgccac tggcggcagg cataaccggt tccatccggt gtcagaaatt tcgaatgcgc
     2761 ttagcaatcc tggcgcgtct ggaaaagtgt gggacagcaa tatgcgaacg gtacgcggca
     2821 aggggttgcg acaacaggat cgtagtcggc agacatgccc ggcacgagcc gctgtaggga
     2881 gggaccaatg acgacaatta gcagcgatgg aacgatgtcg ggcgccaaga aggaaggcat
     2941 cctcgatcgc gagcacacta tcgcaaaggc cggcttcaat cgctggctgg tgccacccgc
     3001 ggcgctttgt attcatctgt gcatcggcat ggcgtatggc ttcagcgtgt tctggttgcc
     3061 gctgtcgcgc gcggtcgggc tcactgcgcc caaagcctgc cccgacatgt cgctgctgca
     3121 ggagctcttc accaccacgt gcgactggcg cgtcgccagc atgggctgga tgttcacgct
     3181 gttcttcgtg ctgctgggcg tttcggccgc gatctggggc ggctggctcg agcgtgtcgg
     3241 cccgcgcaaa gccggcgtcg tggcggcgct gtgctggggc ggcggtctgc tgatcggcgc
     3301 gttcggcgtt tacgttcatc agctctggat catgtggctc ggcgccggcg tcatcggcgg
     3361 cgtcggtctc ggcctcggtt acatctctcc ggtttcgacg ctgatcaaat ggttccccga
     3421 ccatcgcggc atggccaccg gttttgccat cgcaggtttc ggcggcggcg ccatgatcgg
     3481 cgcgccgctg gcgaatttgc tgatcaccta cttcaagacc cccacatcgg tcggcgtctg
     3541 ggagaccttc gccaccatgg gcgtggtcta cttcgtcttc atgatgatcg gcgccttcgc
     3601 ctatcgcgtg tctcccgtcg gctggcagcc tgaaggctgg acggcgcctg cgaaagccaa
     3661 tgcgatgatc tcggcgaaca acgttcatct caacgacgcg cacaagaccc cgcaattctg
     3721 gctgatctgg ggggtgctct gcctgaacgt gtcggccggc atcggcgtga tcggcatggc
     3781 ctcgccgatg ttgcaggaga tattcgccgg caaactg
//
LOCUS       DASWGM010000162         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2508644,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000162 DASWGM010000000
VERSION     DASWGM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2508644"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VGA15_04900"
     CDS             <1..276
                     /locus_tag="VGA15_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9587064.1"
                     /translation="VRIAGTDFTTFIKRSELARERSDQRAERFAVGEKVDARVIQFDK
                     KARKVQVSIKALEVAEEKEAIAQYGSSDSGATLGDILGTALKQRNDK"
     gene            385..1365
                     /gene="sppA"
                     /locus_tag="VGA15_04905"
     CDS             385..1365
                     /gene="sppA"
                     /locus_tag="VGA15_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX9587065.1"
                     /translation="MSLDSDVIVDRRRIRRKLTFWRVVAALVAIAAIVTVGVIATPGG
                     RSAFTASGSIARIHIDGLIRSDYDRVEALERLEKSRAAAVIVHINSPGGTTAGSEQLY
                     DALVRLKAKKPVVVVVEGLAASGGYITAIAADHIIAQQSSLVGSIGVLFQFPNFSELL
                     KTVGVKVEEVKSSPLKAAPNGFEPTSPEARAALDALVKDSYAWFRDLVKARRGMDDAL
                     LEKVADGRVFTGRQAVDLKLIDQLGDEKTAIAWLVAQKNIKSDLPVRDFKLSPRFGDL
                     TFLRTAASITLDALGLGAIARQVEQGGVAQAVDRLGLDGMLALWSPAGSN"
     gene            1537..1842
                     /locus_tag="VGA15_04910"
     CDS             1537..1842
                     /locus_tag="VGA15_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX9587066.1"
                     /translation="MIKSELVQRIAEHNPHLYQRDVENIVNAILDEIVAALARGDRVE
                     LRGFGAFSVKHRPARAGRNPRTGAHVPVDQKSVPFFKTGKEMRERLNREGGAPDAGA"
BASE COUNT          341 a          582 c          592 g          348 t
ORIGIN      
        1 gtccggatcg ccggcaccga cttcaccacc ttcatcaagc ggtccgaact ggcgcgcgag
       61 cgctccgatc agcgcgccga gcgtttcgcc gtcggcgaaa aggtcgacgc gcgggtgatc
      121 cagttcgaca agaaggcccg caaggtgcag gtctcgatca aggcgctgga agtggccgaa
      181 gagaaggagg ccatcgcaca gtacggctcc tccgattcgg gagcgacgct gggcgacatt
      241 ctcggtaccg cgctcaagca gcgcaacgac aagtaagcct ctcgctttct cgcctcaatc
      301 aagacggggc cccggtttcg accggggcct tgggtgacaa ataccgtgca atcccctaaa
      361 gaccgcccct tgggagaatt ttcgatgtcg cttgattcgg acgtgatcgt cgatcgccgc
      421 aggatacgcc gcaagctgac tttctggcgc gtggttgccg cgctggtcgc aatcgctgcg
      481 atcgtcaccg tgggcgtgat cgcaacgccg ggcggccgca gcgcgttcac ggcttccggc
      541 tcgattgccc gcatccacat cgacggcttg atccgcagcg actatgatcg cgtcgaggcg
      601 ctggaacggt tggaaaaatc gcgcgccgcg gcggtcatcg tgcacatcaa ttcgccgggc
      661 ggcaccacgg ccggctccga gcaactctac gacgcgctgg tgcgcctgaa ggcaaagaag
      721 ccggtggtcg tggtcgtcga gggactggcg gcgtcgggcg gctacatcac cgccatcgcg
      781 gccgaccata tcatcgccca gcagagctcg ctggtcggct cgatcggcgt gctgtttcag
      841 ttccccaatt tcagcgagct cttgaagacc gtcggcgtca aggtcgagga agtgaagtct
      901 tcgccgctaa aagccgcgcc caatggcttt gagccgacca gtccggaggc gcgcgcggcg
      961 ctcgacgcgc tggtcaagga ctcctatgct tggttccgcg atctcgtgaa ggcgcggcgc
     1021 ggcatggacg acgccttgct cgagaaagtc gcggacggac gggtcttcac cggccgccag
     1081 gcggtcgatc ttaagttgat cgatcaactc ggcgacgaga aaaccgccat tgcgtggctg
     1141 gtcgcgcaga aaaacatcaa gagcgatctg ccggtgcggg acttcaaact gtcgcctcgg
     1201 ttcggcgacc tgacctttct gcgcacggcg gcttccatta cgctcgacgc gctcggcttg
     1261 ggcgccattg cgcggcaagt cgagcagggc ggcgttgcac aggcggtcga tcgcttaggt
     1321 ctcgatggca tgttggcgtt gtggtctcct gcggggtcga actgagcgca taagtccggt
     1381 tccgatccgc tcctcccgca ttgcggcgta ttcccacgat ccctaatcgg ggcatttgtc
     1441 acgcgttttc gcggcgcgta atctcattta gcgtcttgac agtgcaagcc attttcacgg
     1501 aaatgatatc agcacattcg ccgggtccca gcctcgatga tcaaatccga actcgtccag
     1561 cgcatcgccg agcacaaccc gcatctttat cagcgggatg tggagaacat cgtgaacgcg
     1621 atcctcgatg agatcgtcgc cgctctggcg cggggcgacc gggtcgaact gcgcggtttc
     1681 ggcgcgtttt cggtgaagca ccgcccggcc cgcgccggcc gtaatccgcg caccggggca
     1741 cacgttccag ttgatcagaa gagcgtgccg tttttcaaga ccggcaagga gatgcgcgaa
     1801 cgtctgaacc gcgagggcgg ggcacccgac gccggcgcgt aaagctggta ctatctccag
     1861 taa
//
LOCUS       DASWGM010000163         5387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1677165,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000163 DASWGM010000000
VERSION     DASWGM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677165"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VGA15_04915"
     CDS             complement(<1..347)
                     /locus_tag="VGA15_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587067.1"
                     /translation="MDIRARWLTFYAEHVEQTSKEIFGHVEKMIVGTLIISAGAHVSS
                     NEPAIVLFGYLRHGLVGRGVELFGVILLVLNFLDGFYKLARLEWHAAYQIVMSFCYIV
                     LSVRLIQLILAFRG"
     gene            583..804
                     /locus_tag="VGA15_04920"
     CDS             583..804
                     /locus_tag="VGA15_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587068.1"
                     /translation="MLRAVLIAALLCASEAAFSQELTAEQRSACMGDYKKYCKATKPG
                     GGRIIACLSKSSDKLSAECKKVLAEAEKK"
     gene            978..1193
                     /locus_tag="VGA15_04925"
     CDS             978..1193
                     /locus_tag="VGA15_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587069.1"
                     /translation="MRSFLLIASAILAFAATMTFETNDANAVVCARGIYRAGCAGARG
                     AVVVRRPPVVAACRRVMVNGVWVRRCV"
     gene            1336..4119
                     /locus_tag="VGA15_04930"
     CDS             1336..4119
                     /locus_tag="VGA15_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX9587070.1"
                     /translation="MRIAVIGGGPGGLYFAYLWKKLHPDASIDLFEQNAAGATFGFGV
                     VFSEQALEFLRADDPETVDAIAPRMESWKNITLNLRGESVAIDGVGFSSIGRLDLLTI
                     LQQRAGSVGVTPRYDTPIQSVDQLTGYDLIVAADGLNSLARRTFESEFGASVSHSANQ
                     FAWYGTTKRFETLSQTFVETGLGAFNAHHYRYSPDMSTFLVECAPATWQRYGFADKTP
                     AESQAVCERVFASTLGGHTLVSNKSVWRNFPWIWNERWSFKNIVLIGDALHSAHFSIG
                     SGTRLAIEDAIALAKALDAEADIRDALVRYETERKPIVKKLVTAARTSAGWYENFPEH
                     MKLGLLDFAYSYITRSGRIDEARLRAMSPLFMARYEASKRPLAARREHAMISPRPDEA
                     REIADGVPPDSPGALEIGFAIPQQYNASRILFDNLSKGRGDRLALTGPAGTRSYAELC
                     ADASRWGHGFQALGVKRGDRILMFLDDTPAYPAAFFGAVRSGFVPLLINTLTPADLLQ
                     FYLSDSGATIAVADAEFCARFDAVACQDTLLQTLIVVNGAASDHAVQNTIIAERWLPT
                     FSADLPEADTHRNEMAFWMYSSGSTGRPKGIVHLQHDMAYSEAAFAHHVLKLEVDDIC
                     FSVPKIFFAYGFGNSITFPFAAGAATLLLPGQPKPAAIFEAIARFRPSVFFGLPTLYT
                     ALTKADHAAATDFSSLRMALSAAEVLSADVFNGWKTLTGLEIVEGLGSTEVLHIYLSN
                     RPEQKKLGAAGLRVPGYEIALRDKDGREVADDEEGILWVRGDSNTPLYWNRPDKSAET
                     IREGGWIYTGDRFVRDGDGFHFFRGRADDLIKISGQWVYPLEVELCLAEHPQVRECAV
                     FAAELPDRRMTLKAVVVLNNRALHAADTTKMLQDFVKAKLLPYKYPREIKFIDELPKT
                     GTGKIDRQALMRI"
     gene            complement(4301..>5387)
                     /locus_tag="VGA15_04935"
     CDS             complement(4301..>5387)
                     /locus_tag="VGA15_04935"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HEX9587071.1"
                     /translation="KASALGEIGAGIQLGPNAFHAFDYLGVGEAARSMAVYIDQLRLM
                     DALTAEEITHVDLRDAFRSRFGNPYAVVHRGDLHGVFLRACEAHDLIELRVSSEVLGY
                     DQDGSSVTARLAFGDTVTGSLLIGADGLWSNIRKKVTADGPPRVSGHTTYRSVIPTEE
                     MPEDLRWNAATLWAGPKCHIVHYPLSGWKVFNLVVTCHNDAPEPVAGKPVSDAEVMQG
                     FAHVHARAQDIIRHGNNWRLWVLCDREPTERWIDGRVALLGDAAHPMLQYFAQGACQA
                     MEDAVCLSHMLDAYPDDHTSALEHYRSQRFPRTARVQLLSRAIGEHIYHPSGEHARLR
                     NAIMSAKSSEDYYRDLAWLYGGTGLRS"
BASE COUNT         1079 a         1651 c         1618 g         1039 t
ORIGIN      
        1 ccgcgaaagg cgaggatcaa ctggatcaat cggaccgaca agacaatgta gcagaatgac
       61 atgacgatct ggtacgcggc atgccactcc agccttgcaa gcttataaaa accgtcgagg
      121 aaattcagga ccaggaggat caccccgaac agctcgacgc cgcggccaac caggccatga
      181 cgcagatagc cgaacagaac gatcgccggc tcgttgctcg agacatgcgc gcccgccgag
      241 atgatcaacg tcccgacgat cattttttca acatgaccga atatttcctt ggaagtctgc
      301 tcgacatgct cggcgtagaa cgtaagccac ctggctctga tatccatggt ttctccttat
      361 gactgcggcc ggcatcgggc ggattggctc cggtcgcatg gctcaaacag cgattttccg
      421 gttttcgata ccgggcgttt caataccggg agtttcaata cactggctcc gcgatcaagc
      481 cgcaagaaag cgaatcacct gatttttcca gggagctgcg atgtgacggg gggctacccc
      541 tgcaacacat gatcaactga aaaaacggaa aggaaagtac aaatgctacg cgccgtcctg
      601 attgccgccc tgctgtgcgc gtccgaagcc gcgttttccc aggagctcac cgccgagcaa
      661 cgcagtgctt gcatgggcga ttataaaaaa tattgcaaag ccaccaagcc gggcggcggc
      721 aggatcatcg cctgcctgtc caaatcgagc gacaagctct ccgcggaatg caaaaaagtc
      781 ctggctgaag cagagaaaaa gtaagccgcg caaatggacg gcgtcgaagc ctggaaatct
      841 tcccgggttc ggcgcgcaag taccgcgtcg gagcaaggtc gtttgtgatg atccgcgttg
      901 ggcgcatgcg gaaatcgaaa tgcgtccgta gaaaccgttc cttggctatt caacaaggac
      961 aaatcaggga gagaaacatg cgttcattct tactcattgc ctccgcgatc ctggcctttg
     1021 ccgccaccat gacctttgag acgaacgacg ccaatgcggt ggtctgcgcc cgtggcatct
     1081 atcgggccgg ctgcgccgga gctcgcggtg ccgtggtggt gagacggccc ccggtcgtag
     1141 ccgcctgccg tagagtaatg gtgaacggtg tgtgggtgcg ccgctgcgta tagcgcgagc
     1201 caatctcatc ggataagttg ccgatcacgc tagcttgtta ccctggttga ccgctgcgca
     1261 ccgccgtcgt ggcgatgacg attggcggtc aactccttgt ccgaacaccg cctgtttgca
     1321 tggaggagga agattttgcg catcgccgtg atcggcggag gccccggagg tctctatttc
     1381 gcgtacctct ggaaaaaact ccaccctgac gcatcaatcg atctgttcga acagaatgcc
     1441 gccggggcca cctttgggtt tggggtggta ttttcagagc aggcgctgga attcctgcgc
     1501 gccgatgatc ccgaaacggt cgacgccatc gcaccaagga tggaaagctg gaagaacatt
     1561 accctcaatt tgcgtggcga aagcgtcgcg atcgacggcg tcggcttctc ctcgatcggc
     1621 cggctcgatc tgctcaccat cctgcagcag cgcgcaggct cggtcggggt cacgccgcga
     1681 tacgatacgc cgatccagtc cgtggatcag ctgaccggtt acgatctgat tgtcgccgcc
     1741 gatggcctga attcgctggc gcgtcgcacc ttcgaaagcg aattcggcgc gtcggtgtcc
     1801 cattcggcca atcaatttgc ctggtacggc accacaaaac ggttcgagac actgtcgcag
     1861 acctttgtgg aaaccgggct cggcgcgttc aacgcccatc actatcgata ctcgccggac
     1921 atgagtacgt tcctggtgga atgcgccccc gcgacctggc agcgctacgg ctttgccgac
     1981 aagacacctg cggaatcgca agccgtttgc gaacgcgtct tcgcttccac gctcggcgga
     2041 catacattgg tgtcgaacaa atcggtttgg cgcaactttc cgtggatctg gaacgagcgc
     2101 tggtcgttca aaaatatcgt actgatcggc gacgcgctgc actcggcgca tttctcgatc
     2161 gggtcgggca cccggctcgc catcgaggac gcgatcgcgc tggccaaggc gctggatgcg
     2221 gaagcggata tccgtgatgc ccttgtccgt tacgaaaccg agcgcaagcc gatcgtgaaa
     2281 aagctggtga cggcggcgcg caccagcgcc ggctggtacg agaatttccc cgagcatatg
     2341 aagctcggcc ttttggattt tgcctatagc tatatcaccc ggtccgggcg gatcgacgag
     2401 gcgcgcctgc gcgcgatgtc gccattgttc atggcgcgct acgaggcatc gaaacgtccc
     2461 cttgcagcac ggcgggagca cgcgatgatt tcgcccaggc cggacgaggc gcgagagatc
     2521 gccgacggtg tcccacccga cagcccgggc gccttggaga tcggctttgc gatcccgcaa
     2581 caatacaacg ccagccgtat tctgttcgac aacctgtcga agggtcgcgg cgaccgcctc
     2641 gcgctcaccg gcccggctgg cacgcgcagc tatgccgaac tctgtgccga tgcctcccgc
     2701 tggggccacg gttttcaagc gctgggcgtc aagcgcggcg accgcatcct gatgttcctt
     2761 gacgacaccc cggcctatcc agccgcgttt ttcggcgcgg ttcgctcagg cttcgtgccg
     2821 ctcttgatca acacgctgac gccagctgat ctgctgcagt tttatctctc ggattccgga
     2881 gcaaccatcg cggtcgccga cgccgaattt tgcgcgcgct tcgacgctgt ggcctgccag
     2941 gatacgctgc tgcaaacact gatcgtcgtc aacggcgccg ccagcgatca cgccgtgcaa
     3001 aataccatca tcgccgagcg gtggttgccg actttttccg ccgatctccc tgaagccgac
     3061 acgcaccgca acgagatggc gttctggatg tattcgtcgg gctcgaccgg acgtcccaag
     3121 ggcatcgtcc atctgcagca cgatatggcc tatagcgagg ccgcgttcgc gcaccatgtg
     3181 ctgaagcttg aagtcgacga catctgcttt tcggtaccaa aaatcttttt cgcctatggg
     3241 tttggcaatt ccatcacctt ccctttcgca gcgggcgcgg cgacgctgct gctgccgggc
     3301 cagcccaagc cggcggcgat ctttgaggcg atcgcgcgct tcagaccctc cgtcttcttc
     3361 ggattgccga cgctctacac agccctgaca aaggccgacc acgcggcggc gacggatttt
     3421 tcctcgctgc gcatggcgct gtccgccgcc gaggtgctgt cggccgatgt cttcaacggc
     3481 tggaagaccc tcaccggcct tgagatcgtc gaagggctgg gctccacgga agtgctgcac
     3541 atctacctca gcaatcggcc ggagcagaaa aaactcggcg ccgcgggtct tcgggtgccc
     3601 ggctatgaaa tcgcactcag ggacaaagac ggccgcgagg tcgccgacga cgaagaaggg
     3661 atcctttggg tccgcggcga ttccaacacg ccgctgtact ggaaccggcc ggataaatca
     3721 gccgagacca tccgcgaggg gggctggatc tacaccggcg accgttttgt ccgcgacggc
     3781 gacggctttc attttttccg cggccgcgcc gacgacctga tcaagatttc cggccaatgg
     3841 gtctacccgc tcgaagtcga gctttgcctc gccgagcatc cgcaagtgcg cgaatgcgcg
     3901 gtgttcgcgg cggaattgcc ggaccggcgc atgacgttga aggcggtcgt tgtcttgaac
     3961 aaccgtgcgc tccatgcagc ggacacgaca aaaatgctgc aggattttgt caaggcaaag
     4021 ctattgccct acaaatatcc acgcgagata aaattcatcg acgaattgcc gaagacaggc
     4081 accggcaaga tcgaccggca agcattgatg aggatttagc gggctagcgc gtcaacatat
     4141 tcagcgtcgt ccccgcctag tgcgcaattg cgcacggggg gcggggaccc ataccccgtg
     4201 ccgtctcata ttcaataatg cctttggacc cattcgtcgc aacatcgacg ctggaggtta
     4261 tgggtcccgg ctcaaggccg ggacgacgga aggtttgcgt ttaactccgc agccccgtgc
     4321 cgccatagag ccaggcgaga tcgcgataat aatcctccga cgacttcgcg ctcatgatgg
     4381 cgttgcggag gcgggcgtgt tcgccggagg gatgataaat gtgttcgcca atggcgcggg
     4441 ataatagttg aacgcgcgcg gtgcggggaa aacgctgcga acgatagtgc tcgagcgcgc
     4501 ttgtgtgatc atccggataa gcatccagca tgtgcgacag gcacaccgcg tcttccatgg
     4561 cctggcaggc gccttgcgcg aaatattgca gcatcggatg cgcggcgtcg ccgagcagcg
     4621 cgacgcggcc gtcgatccag cgctcggtgg gctcgcggtc gcacagcacc cagagccgcc
     4681 agttgttgcc gtggcgaatg atatcttgcg cgcgcgcatg gacatgcgcg aaaccctgca
     4741 tcacctcggc gtcggacacc ggttttcccg ccaccggctc cggggcgtcg ttgtgacagg
     4801 tgacgaccag attgaacact ttccagcccg acagcggata gtgcacgatg tggcatttcg
     4861 gccccgccca cagcgtcgcc gcgttccagc gcagatcttc cggcatctcc tcggtcggaa
     4921 ttaccgaacg ataggtggtg tggccggaaa cgcgcggcgg cccgtcggcg gtgacttttt
     4981 ttctgatgtt cgaccacaaa ccatccgcgc cgatcagcag cgatccggtg acggtgtcgc
     5041 cgaaggcaag ccgcgcggtg acggaagagc catcctggtc ataccccaaa acttcgctgc
     5101 tgacgcgcag ctcgatcagg tcgtgagcct cgcaggccct cagaaatacg ccgtgcagat
     5161 cgccgcgatg aaccaccgcg taggggttgc cgaagcgcga gcggaacgcg tcgcgcagat
     5221 cgacatgggt gatctcctcg gcggtgagcg catccatcag ccgcagctga tcgatataga
     5281 ccgccatgct ccgcgcggcc tcgccgacgc cgagatagtc gaaggcatga aacgcgttgg
     5341 ggccgagctg gatgccggcg ccgatttcgc cgagcgcgga tgccttt
//
LOCUS       DASWGM010000164        12899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2233332,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000164 DASWGM010000000
VERSION     DASWGM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12899
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233332"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VGA15_04940"
     CDS             complement(<1..275)
                     /locus_tag="VGA15_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX9587072.1"
                     /translation="MTDTHYDRAPGDASALPDVEQGTSSGVLVYTIGLVLAVVLTAMS
                     FWVANTSLFWAGGVSLGLTVLAIAQMGIHLVFFLHVTTGPDNTNNVMA"
     gene            complement(272..907)
                     /gene="cyoC"
                     /locus_tag="VGA15_04945"
     CDS             complement(272..907)
                     /gene="cyoC"
                     /locus_tag="VGA15_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEX9587073.1"
                     /translation="MNIAVALDDEVRQEARPRASEAGPATKRIVVGYGFWIFLLSDIV
                     MFAAFFAAYAVLAHATAGGPSGLQLFNQRSVAIETGCLLASSYTCGLMSLVIGSRRYP
                     ATYLAALITFVLGAAFLILEVREFAGMIAVGAGPQRSAFLSAFFTLVGCHGLHVTAGL
                     VWLVVMMAQVAIKGFRETVERRLLCFSLFWHALDIVWVWLFTVVYLMGVLS"
     gene            complement(904..2889)
                     /gene="cyoB"
                     /locus_tag="VGA15_04950"
     CDS             complement(904..2889)
                     /gene="cyoB"
                     /locus_tag="VGA15_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEX9587074.1"
                     /translation="MNLLGKLNWNAIPFDQPIIMGASGGMVLVIVSILSWVTLKGYVP
                     YLWREWITSVDHKRIGIMYIILALIMLLRGFADAIMMRAQQAVAAGGAQGYLPPEHFD
                     QIFSAHGTIMIFFMAMPFVIGMMNFVVPLQLGVRDMAFPTLNSVALGLTASGILLTNI
                     SLTVGEFAKTGWVAYPPLSELQFSPGVGVDYYLWALQISGVGTLMTGINFVATILKTR
                     APGMSYMRMPVFCWTSLAANLLVVAAFPVLTATLAMLLLDRYLGFHFFTIDGQGNAMM
                     YVNLFWVWGHPEVYILVLPAFGIFSEVVSTFSGKPLFGYRSMVAATMAICVLSFIVWL
                     HHFFTMGASANVNGFFGVMTMIIAVPTGVKVFNWLFTMYGGRIRFTVPILWSIGFMVT
                     FVIGGMTGVLMAVPPADFQLHNSLFLIAHFHNVIIGGTVFGLMAGYNYWFPKAFGFKL
                     DERWGRASFWCWLIGFYLAFMPLYALGLMGMTRRMQHYDVASWQPWLVVAAVGVVVIL
                     AGIVCQVIQLVVSIRDREQLKVTADPWNGRTLEWATASPPPAWNFAMLPQVTDIDAFW
                     KRKQRERTQQAQPPKEHKFEPIEMPKNSATGFVTAFFSVITGFALIWHIWWMAGAGAI
                     GIFLTMLAFAFRHEEEIKIPVDQIARFEQANQAEIAV"
     gene            complement(2886..3764)
                     /gene="cyoA"
                     /locus_tag="VGA15_04955"
     CDS             complement(2886..3764)
                     /gene="cyoA"
                     /locus_tag="VGA15_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEX9587075.1"
                     /translation="MRYGLLVVVLIGVATLGGCSDGVLDPKGPIAAADRQILLNALGI
                     MLAIVIPVILATLGVAFWFRASNERARYRPNFAYSGRLEMLVWSIPAMTVFLVGGVAW
                     VGSHDLSPRKPIASTVKPLRVQVASLDWKWLFIYPDQGVASVNRLTIPVGTPVSLELT
                     SSGVMNSFFVPQLGSQIYTMAGMITRLHLQADHPGTYRGFSAQFSGEGFSDMHFDADA
                     VTNEKFAQWLDSVRIAGPELDAKTYADLAKPSASVTPFTYRSVAPGLFASILVSEMQS
                     DDAMCRRYPTSMRAEK"
     gene            complement(3766..4827)
                     /locus_tag="VGA15_04960"
     CDS             complement(3766..4827)
                     /locus_tag="VGA15_04960"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9587076.1"
                     /translation="MNVKSIAGQLGERRARFALSSDSAHPLADHWSVSDFVALTKPRV
                     MLLAVFTALVGLSSAPVRLDPLTTLAAVLAIAAGAGAAGVLNMWYDADIDAVMTRTAM
                     RPIPRGKISRFEALVFGLVLAGFAVVVLALATNLTAAALLAGTILFYIVVYTAWLKRA
                     TPQNIVIGGAAGALPPVIGWAAATGDVGLEPLALFLIIFLWTPPHFWALALNRTDDYA
                     RAGVPMLPVIAGRAATTRQILSYSGLLVLASELPWMLGFAGAIYGVIVAICGALFLLL
                     ARQLNRSLEADRRAAHRLFVFSIFYLFVLFAALLIDHGTGSFSPTQSSHGGRGVSVHA
                     ELLPGAVRSACCTANFSEV"
     gene            5509..6813
                     /locus_tag="VGA15_04965"
     CDS             5509..6813
                     /locus_tag="VGA15_04965"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX9587077.1"
                     /translation="MLWLLDRATQLTNLKRETPCPEPLTCEAALKTTALAATFLFAHG
                     QTTERTVTDAERLGRALGVTVRVLPYWGELTVELDGAPISQIVPAKPLGVDMGRVLAV
                     TTVIDQVCHGTLSAEAAQSALESAGRLKPASTPRFTLFAAIGAASLGVIFGALDATSL
                     LLIAASAAIGALVRRWLSGFSNNPLIQPLCAAFVAGVVAAAASRFQLSDAITLVGFCP
                     CMVLVPGPHILNGAIDLARTRIALGIARLAYAGVIVLLICAGGLFGFTAVGANLTIAG
                     PSPRVPLVIDVIAAGCAVASFGTFFSMPWRLLALPIAAGMLAHAARWALISVAGTNAA
                     TGALVACMLVSVLVTPIVDRLHLPFAALAFSAVVSLMPGFFLFNAATGLVELVSIGPT
                     APAALLTSIATSGATAFLIIMAMTFGLILPRMLFERVLPAPA"
     gene            7003..8256
                     /locus_tag="VGA15_04970"
     CDS             7003..8256
                     /locus_tag="VGA15_04970"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017961208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9587078.1"
                     /translation="MFVGGGFAGIAAARALRRANVEITLVDRRNHHIFQPLLYQVATA
                     VLSPSEIAAPIRQLEAKQKNLNVLLADVKGINLGTRTVEAVCPGIGGRKLEYDFLVIA
                     TGMRPSYFGHDEFAQFAPGLKSLNDAETIRTKILSAFELAESTDDPKERARQMTFVLV
                     GAGPTGVELAASIAQLAAVTLRRNFRKIDPAKSRIVLLDGGTRILPTFAESLSQKAAK
                     RLTKLGVEVSTGVKVEKVDDQGVIAADVRIPSATVLWTAGVSASTVVKMLGTQTDRAG
                     RAVIGAFMDIPEAPNVFVAGDAATMTQDGHPVPGVAQAAIQQGRFVGHVIANRVRGRK
                     DGRPFRYRNKGNMAVVGKNFAILESGHFRSSGFMTWLVWAVLHVLALPQLQNRFRVQT
                     QWFWSYLSGQRSSRLISEGPRSTAS"
     gene            8317..8766
                     /locus_tag="VGA15_04975"
     CDS             8317..8766
                     /locus_tag="VGA15_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587079.1"
                     /translation="MDTSIISALAALTGAAVGGLTSGIANWLNHRSQVRAQWILHEKS
                     RRQILYRDFIEEAAKCYIDALQHDEADIPGLVGLYAKLSRMRTLSSKPVVHHAEDVAR
                     KILDTYLEPDKSFVELREMAIDGTIDLLRDFSDACHDEFEEMRPIQF"
     gene            complement(8856..9437)
                     /locus_tag="VGA15_04980"
     CDS             complement(8856..9437)
                     /locus_tag="VGA15_04980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX9587080.1"
                     /translation="MTVDKPMNVSVLVGSLRKASLNGMLANALISLAPSSMKLETVEI
                     GQLPFFNQDLETAATPAPRTAFRQRVKAADAILFVTPEYNRSVPAALKNALDVGSRPY
                     GSNVWDRKPGAIVSCSPSAIGAFGANHHLRQSLVFLNVPTMQQPEAYVGHADKLFDEH
                     GKLVNDGTGKFLQAFMQAFANWVETTRSQGQRI"
     gene            complement(9434..10219)
                     /locus_tag="VGA15_04985"
     CDS             complement(9434..10219)
                     /locus_tag="VGA15_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9587081.1"
                     /translation="MDDIVTEDAGSILRVQFNRPTKRNAMTSAMYAALAGIFNKAAND
                     ANTRVVLWHGAGDSFCAGNDIEDFLKNPPGPGESPQAGLMNAFVNFDKPVVVAVHGAA
                     IGGGTTMLTHCDFIYAGEGTKFQMPFINLAVVPEFGSSCSVPARIGHVRAAELLLLGA
                     PFDARHAAELGLVTEVVSDKDVLTRATETARKLAAKPAAALQASKRLMKQPFREQIKA
                     AMKAENEEFSAQVRSDDAKESFSAFLEKRKPDFARTVKSPATA"
     gene            complement(10303..11529)
                     /locus_tag="VGA15_04990"
     CDS             complement(10303..11529)
                     /locus_tag="VGA15_04990"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9587082.1"
                     /translation="MASDNIFSGLKVVDLASFIAGPSAAVILSDFGADVIKVEPPSGE
                     LWRHAHNMPPQPVAEDPYPWHLANRNKRGMALDLKSPSAHAVLEKLVKWADVLIVNTP
                     HPARARLKLNYEDVMPWNPRLIYADITGFGDKGPDADLPGFDITSYWARSGLLSMTRD
                     AGAPPTWPVAGSGDNATAVGLYSAIVTALYRRERTGKGAHVTTSLLAEGVWSASVSIQ
                     AALCEAKFFGLHDRMHPANAAMNVYRAKDGTWFVLIVTPDKLAAVAKALGRADLLTDP
                     RFSDPAKLTANMPQLTAILDEIFCGEPMAHWYEMFNGVHVTFGAVRGPQEVINDPQLQ
                     ANDIIVPLNGAGGKLTSTISSPLQVHGVAKVPAKRAPKIGEHNDEVLRQLGFSATEID
                     GLRASGAIPKPKEHAA"
     gene            complement(11559..12410)
                     /locus_tag="VGA15_04995"
     CDS             complement(11559..12410)
                     /locus_tag="VGA15_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9587083.1"
                     /translation="MVSGTAPLTPSPATVPNSLPRAALTLANVLYEKNGSIAYVTVNR
                     PKVLNALNTPTWTDLRAAFEDAKADASVHGVILTGAGDKAFIAGADISELANVDAYDA
                     EESSRFGQGVLDLIEDLGKPVIAAINGFALGGGCETAMACTIRIAAEHARFGQPEVKL
                     GLLPGGGGTQRLPRLVGKGRALQLILTGETISAQEAYRIGLVNEVVSAAGLIDRAETI
                     LKQIMANAPIAVKFSLQAANKGMDTSQTEGFALEASYFGICAATEDKKEGTSAFLEKR
                     APKFRGR"
     gene            complement(12470..>12899)
                     /locus_tag="VGA15_05000"
     CDS             complement(12470..>12899)
                     /locus_tag="VGA15_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9587084.1"
                     /translation="RYMVGWASTGIQMGAFEATVKYAQERQQFGKPIASFQMIQDLLA
                     KMLANLTACQCLMLRLAQLDDEGKLGDHHAALAKAFCTAKSRETVSWGREVLGGNGIV
                     ADYNVARFFADAEALYSYEGTYQMQNLIVGKAITGHGAFI"
BASE COUNT         2638 a         3939 c         3875 g         2447 t
ORIGIN      
        1 gccatcacgt tgttggtgtt gtctgggccc gtggtgacgt gcaggaaaaa caccaggtgg
       61 atacccattt gcgcgatcgc gaggacagtc aggccgagag agacgccgcc tgcccagaac
      121 agtgacgtgt tggcgaccca gaacgacatg gccgtgagga ccaccgcgag aaccaatccg
      181 atcgtgtaga caagcacgcc ggacgacgtg ccctgttcca cgtcgggaag tgctgacgcg
      241 tctcccggtg cccgatcgta atgcgtgtcg gtcatgaaag aactcccatc agatagacca
      301 ccgtgaacag ccatacccag acaatgtcga gcgcatgcca gaacagtgaa aagcagagca
      361 gccgtcgctc cacggtctcg cgaaaaccct tgatggcgac ctgagccatc atcaccacca
      421 gccagaccag gcccgcggtg acatgcagcc cgtgacagcc gacaagggta aaaaacgcgg
      481 agaggaaagc gctgcgttgc ggaccggctc cgacggcgat cataccagcg aactcccgca
      541 cctccagtat caggaacgcg gctcccagca cgaaggtgat gagggcagcc aaataagtgg
      601 cgggatagcg ccgcgagccg atcaccaggg acatcaatcc gcaggtgtag ctcgacgcga
      661 gaaggcagcc ggtttcgatc gcaacgctac gttggttgaa cagctgcaag ccgctcggac
      721 cgccggcggt cgcatgcgcg agcaccgcgt aggcggcaaa gaacgccgca aacatcacga
      781 tgtcgctcaa caggaatatc cagaagccgt aaccaaccac gatccgcttt gtagcggggc
      841 cggcctcgct tgctctcggc cgcgcctcct gacgaacctc atcatcaagt gcaacagcga
      901 tgttcatact gcaatctccg cctggttcgc ctgctcgaaa cgcgcaatct gatcgaccgg
      961 gattttgatt tcctcttcat ggcgaaacgc gaaggcaagc atggtcagaa agattccgat
     1021 tgccccagca cccgccatcc accagatgtg ccagatcagc gcaaagccgg taatgactga
     1081 aaagaacgcc gtgacgaagc cggtcgcgct gttcttcggc atctcgatcg gctcgaattt
     1141 gtgctccttc ggcggctggg cttgttgagt gcgctcgcgc tgcttccttt tccagaatgc
     1201 gtcgatgtct gtgacttgcg gcagcatggc gaaattccag gctggcggcg gcgaagcagt
     1261 cgcccattcc agcgtgcggc cgttccatgg gtccgcggtc accttcagtt gttcgcggtc
     1321 gcggatcgag accacgagtt ggatgacctg gcagacgatg ccagcgagaa tgacgacgac
     1381 gccgaccgcg gcgaccacca accacggttg ccaactggca acgtcgtaat gctgcatgcg
     1441 gcgggtcatg cccatgagac ccagcgcgta gagcggcatg aaagcgagat agaagccgat
     1501 cagccagcac cagaatgacg ctctgcccca gcgttcgtcg agcttgaaac cgaatgcctt
     1561 cgggaaccag tagttgtaac ccgccatcaa gccgaacacc gtgccaccga tgatgacatt
     1621 atggaaatgc gcgatcagga ataggctgtt gtgcagctgg aagtcggcgg gaggcacggc
     1681 catcaacacg ccggtcatgc cgccgatcac gaaagtcacc atgaaaccga tcgaccacag
     1741 gatcggaacg gtgaaccgga tgcggccgcc gtacatcgta aacagccagt tgaacacctt
     1801 gacgcccgtc ggcaccgcga tgatcatcgt catgaccccg aagaaaccgt tgacgttcgc
     1861 gctcgcgccc atggtgaaaa agtggtgcag ccagacaatg aatgacagca cgcaaatcgc
     1921 catcgtcgcg gcaaccatgg agcgatagcc aaacagcggc ttgccggaga acgtcgagac
     1981 aacctcagaa aagataccga acgccggcaa gaccaggatg tagacctccg ggtgacccca
     2041 aacccagaac aggttgacat acatcatcgc gttgccctgg ccgtcaatcg tgaagaaatg
     2101 aaagccgagg taacgatcga gcagcagcat cgccagggtc gcagtcagaa ccggaaaggc
     2161 cgcgacaacg agaaggttcg cggcgagcga cgtccagcaa aacacgggca tgcgcatata
     2221 gctcatcccc ggtgcgcgcg ttttcaggat cgtcgcgacg aagtttatgc cggtcatcaa
     2281 cgtgccgacg ccggagattt gcagcgccca gagataatag tcgacgccga cgccgggcga
     2341 gaattgcagc tcgctcagcg gcggataggc gacccagcct gtcttcgcga actcgccgac
     2401 cgtgagcgaa atattcgtca agaggatacc cgacgcggtc agccccaggg ccaccgagtt
     2461 caaggtcgga aacgccatat cgcggacgcc gagctgcagc ggcacgacga aattcatcat
     2521 tccgatcacg aacggcatcg ccatgaagaa gatcatgatc gttccatgcg ccgagaatat
     2581 ctggtcgaag tgctcgggtg gcaagtatcc ctgtgcgccg cccgcggcga ccgcctgctg
     2641 cgcccgcatc atgatggcgt cggcaaagcc gcgcagcagc atgatcagcg ccagtatgat
     2701 gtacatgatg ccgatgcgtt tatggtcgac tgaggtgatc cactctcgcc agagataagg
     2761 cacgtaacct ttgagcgtga cccacgaaag gatcgatacg atgaccagca ccatgccgcc
     2821 cgacgcgccc atgatgatcg gctggtcgaa gggaatcgca ttccaattca gcttgccaag
     2881 tagattcatt tttccgccct cattgatgtc ggatacctgc ggcacatggc atcgtcggat
     2941 tgcatttcgg agaccaggat gctggcgaac aggccgggcg cgaccgagcg ataggtgaat
     3001 ggcgtgaccg acgcgctcgg tttcgccaga tctgcatatg tcttcgcgtc aagctccgga
     3061 ccggcgatgc gcactgaatc aagccactgc gcgaacttct cgttggtcac ggcatcagcg
     3121 tcgaaatgca tgtcggaaaa gccctcaccg ctgaactggg ccgaaaaccc gcggtacgtt
     3181 ccaggatggt cggcctgcag gtgaagacgg gtgatcattc ccgccatggt gtagatctgg
     3241 ctgccaagct gcggcacaaa gaagctgttc atcacgcccg aagacgtcag ctccagactg
     3301 accggcgtgc cgacgggaat ggtcagccga ttgacgcttg cgacgccctg atcgggatag
     3361 atgaacagcc atttccagtc gagcgaggcg acctggaccc gcagcggctt cacggtggac
     3421 gcgatcggtt tgcgtgggct caagtcatgt gagcccaccc acgccacgcc gccgaccagg
     3481 aacacggtca tggcggggat cgaccagacg agcatctcga ggcggcctga atacgcgaaa
     3541 ttcggccgat accgcgcacg ctcattcgat gcacgaaacc agaaagcaac gccaagtgtg
     3601 gcaaggatca ccggaatcac gatcgccagc atgatgccaa gcgcgttgag cagaatttgc
     3661 cggtcggcgg cggcaatcgg ccctttcggg tcgagcacgc cgtccgagca gccaccgagt
     3721 gtcgcgacac cgatcaggac gacgacaagt agaccgtatc gcatgttaga cctcgctgaa
     3781 gttggcggtg cagcatgcgc tgcgaacggc gcctggcagg agttcggcat gcaccgagac
     3841 gccgcggcca ccatgcgacg actgcgtggg cgagaatgag ccagttccat ggtcgatcag
     3901 gagggccgca aacagcacaa acagatagaa gatggagaac acgaacaacc tgtgggcagc
     3961 gcggcgatcg gcctcaaggc ttctgttcag ctggcgtgca agcagaagga aaagcgcccc
     4021 gcagatcgca acgatcacgc catagatcgc ccctgcaaac ccgagcatcc agggcaactc
     4081 cgaggcgaga accagcaggc cgctgtagct tagaatctgg cgcgtggttg cagcacgtcc
     4141 cgcgataacc ggcaacatcg gcacgccagc acgggcatag tcatcggtgc gattgagcgc
     4201 gagagcccag aaatggggcg gcgtccaaag gaagatgatg agaaacagcg caagcggctc
     4261 gagcccgacg tctccggttg ccgcggccca tccgatcacg ggcggcagcg ctcctgcagc
     4321 gccgccgata acgatgttct gtggcgtagc ccgcttcagc cacgccgtgt acacgacaat
     4381 ataaaagagg atagtgccgg ccaacagtgc agcggccgtg agattcgtcg ccaaagccag
     4441 aaccaccact gcgaacccgg ccagaacaag gccaaagaca agtgcctcga accgggagat
     4501 tttgccgcgg ggaattggcc gcatcgcagt gcgggtcatg accgcatcga tatcggcgtc
     4561 gtaccacatg ttgagcacgc cggcggctcc agcccccgcg gcaatggcaa gaaccgcggc
     4621 aagagtggtt agcggatcaa gtcgaactgg agcgctgctc aatccgacga gcgcggtgaa
     4681 gacggcaagc aacatcacac gtggctttgt gagggcgacg aagtcagaca cactccaatg
     4741 gtcagccagc ggatgagcag aatcagacga taatgcaaag cgagctcgtc tttccccaag
     4801 ctggccagca atggatttga cgttcatctc gctgctccga gttctttcaa aatgagagct
     4861 gtcgtgttca gttcgaacag ccgcggtgcg tgcattcaat atgcatcccc accctcggcc
     4921 gcgattgaac gcggctgctc gattggggcc aatcatgctc aaaaaaactg tcagctgacg
     4981 agttcggatc taaacacacg ccgctttgcc atcgcacgat tcaggataat ccgagcacgt
     5041 ttcaacaagc gctctatgcc agcttttcct aaaggagatc atcgttcgga tatctgaaac
     5101 gcgccgctgc ccgattcgaa tatcggacga aatctgttgc gtttgctcgg gagcgaccgc
     5161 ctaacctaac ggagatgtat caatgaagac gccttcgctc tggatgattg cagcagtttg
     5221 ctttgtggcc ccggcatctg tggcccacgc gcgatccgcg tatgatggct cctgggatct
     5281 cgccttcgtg acgcaaaggg gcacatgcga tcccagttac aacttcaccg tcaatgtttc
     5341 agacggcatt gtgactcatc ccaatctcgt aagattcaag ggatacgtcg cgaaatctgg
     5401 ttcggttcgt gcgtcggtga cggttcacga caaatttgcg tccggcacgg gcaggttttt
     5461 cgggacgtcc ggtcgcggaa gatggagcgg ccgcgccgga agtgcgcggt gctctggcta
     5521 ttggaccgcg caacgcaatt gaccaacttg aaaagggaaa caccgtgccc tgagccgctg
     5581 acgtgcgagg cggcattaaa gacgaccgcg ctggcagcca cattcctgtt tgctcacggg
     5641 cagaccacag aacggacggt taccgatgcc gagcggcttg ggcgcgctct cggcgtgacg
     5701 gttagggtgc taccttactg gggcgagctc accgtcgaac tcgacggcgc gcccatctca
     5761 caaatcgtcc ccgccaagcc gctcggtgtc gacatgggca gggtactggc cgtcacgacg
     5821 gtgatcgatc aggtctgcca cggcacgttg tcggccgagg ctgcacaatc cgcgcttgag
     5881 tcggctggcc ggcttaagcc cgcttccacg ccacgcttca ctttgttcgc agccattggc
     5941 gcggcttcgc ttggcgtcat tttcggcgcg ctggatgcga cgagcctgct gctgatcgca
     6001 gccagtgccg ccatcggcgc gctggtcagg cgctggctgt ccggcttcag caataatcca
     6061 ctgatccagc cgctttgtgc cgcgtttgtc gcgggtgtcg tggctgctgc tgccagccgt
     6121 ttccagctat cggatgcaat aacgctggtc ggtttctgtc cctgcatggt gctggttcct
     6181 ggcccgcata tcctcaacgg tgccatcgac cttgctcgca cgcgcatcgc gctgggaatc
     6241 gcgcgcctgg cctatgccgg cgtcatcgtg ctcctgatct gcgccggcgg cctcttcggg
     6301 ttcacggcgg tcggcgcaaa cctcacgata gccggaccat ctccacgcgt gcccttggtc
     6361 atcgacgtga ttgcggcagg atgcgccgtc gcctcctttg gcactttctt ctccatgccg
     6421 tggcgtctgc tcgcgctccc gatcgcggcc ggcatgctcg cgcatgccgc acgctgggcg
     6481 ctgatctccg tcgcggggac aaatgccgcg accggcgcgc tcgtcgcctg catgctcgtc
     6541 agcgtcctcg tgacgccgat cgtcgatcgc ctgcatctgc cctttgcggc tctcgccttc
     6601 tccgccgtcg tctcattgat gccgggcttt ttcctgttca acgcggcgac cggccttgtc
     6661 gaattggtct cgatcgggcc gaccgcgccg gcggctctgc tgacgagcat cgccacgagc
     6721 ggcgccaccg catttctgat catcatggcc atgaccttcg ggctgatcct cccgcgcatg
     6781 ctgttcgagc gcgtattgcc cgcgccggca tgaaaagcgg catggacgtt ctatgcaccg
     6841 caccgccgtt caagattagc ccgaagcccc gttgtatctg atgctaatcg ggcggctggc
     6901 cccagaaaaa ccgtcggtac atccatgact acagttctga ccaaatcgtt cccggaagaa
     6961 aagccacccg taacaaagac ggtcccgggg aaaaaacgcg tcttgttcgt cggcggcggt
     7021 ttcgccggca tcgcggcagc acgggcgctc aggcgagcca atgtcgaaat aacgctggtc
     7081 gaccgtcgta atcaccatat ctttcagccg ctgttgtatc aggtcgccac cgctgttttg
     7141 tcaccctccg agatagcggc gccgatccgt cagcttgagg cgaagcagaa gaacctcaac
     7201 gtgttgctgg cggacgttaa gggcatcaat ctcggaactc gcaccgtcga ggctgtttgc
     7261 ccggggattg gcggtcgaaa actcgagtac gacttcctgg tgatcgcgac tggaatgcgt
     7321 ccgagctatt tcggtcacga cgagttcgcg cagttcgcgc caggcctcaa gagcctcaac
     7381 gacgctgaaa cgatccgaac caagatcctg agtgcgtttg aattggcgga gtcgaccgat
     7441 gacccgaaag aacgcgctcg tcaaatgacc ttcgtgttgg tgggcgctgg cccgaccggc
     7501 gtcgagctcg ccgcttcaat agctcaactg gctgcggtga ctctgcgcag aaatttccgc
     7561 aagatcgacc ctgccaagag ccgaattgtg ttactggacg gcggcactcg catccttccg
     7621 acctttgcag agtcgctgtc acaaaaggca gcaaagcgac taacgaagct cggcgttgaa
     7681 gtttcgacgg gcgtaaaggt cgagaaggtt gatgaccagg gcgtgatcgc ggctgacgtc
     7741 cggattccca gcgccaccgt gctttggaca gcaggcgtca gcgcgtcgac ggtcgtgaaa
     7801 atgctcggga cgcagacgga ccgcgccggg cgcgcagtca tcggcgcttt catggacatt
     7861 cccgaggcgc ccaacgtgtt tgtggcgggc gatgcagcca ccatgaccca ggatggtcac
     7921 cccgtccccg gagtggcgca ggcagccatt caacagggac gatttgtcgg acatgtcatc
     7981 gccaaccgtg tcagggggcg caaggatggt cgaccgtttc gataccgcaa caaaggcaac
     8041 atggcggtgg tcggcaagaa ttttgccatc ctcgagtccg gccatttccg cagcagcggc
     8101 tttatgacct ggctggtgtg ggccgtgctg cacgtgctcg cgctgccgca gcttcagaac
     8161 cggttccgcg ttcaaacgca atggttctgg tcctatctgt cggggcaacg gagctcacgg
     8221 ctgatttccg aagggccgcg gtcgaccgcg tcgtagtagc ggtcaggccc ggtagtcatt
     8281 actgccggat tctgcggctg gttgaggact gcacctgtgg acacctccat catctccgcg
     8341 ctcgccgccc tcaccggagc agctgttggc ggcttgacga gcgggattgc gaattggctg
     8401 aaccaccgaa gccaggtccg cgcccagtgg attcttcacg agaagagccg tcggcagatc
     8461 ctttacaggg atttcatcga ggaagccgcc aaatgttaca ttgatgcgct ccagcacgat
     8521 gaagccgata ttcccggcct tgtcggtctt tacgccaagc tgagcaggat gcggacgctg
     8581 tcttcaaagc cagtggtcca tcatgccgaa gatgttgccc gaaaaatcct ggacacctat
     8641 ttggagcccg acaagagctt cgtcgaactg cgcgaaatgg ccatcgatgg cacgatcgat
     8701 ctgttgcgag acttcagcga cgcgtgtcac gacgaattcg aggaaatgcg gccaatacaa
     8761 ttttgagctt caccttgcgg ttttcatccg cttctttcag cattcgcagt cgcctttgat
     8821 cggataccgc gttcgccagc gcttgccgca aggcgtcaga tgcgctggcc ctgagatcgg
     8881 gtcgtctcga cccaatttgc gaacgcctgc atgaacgctt gcaggaattt tccggtacca
     8941 tcgttgacga gcttgccatg ttcatcgaac agcttgtcgg catgtccgac ataggcttcg
     9001 ggctgctgca tggtcggcac gttcagaaac acgagcgact gcctcagatg atgattggca
     9061 ccgaaggccc cgatcgcgct cggcgagcag ctgacaatcg caccgggctt gcggtcccag
     9121 acgttgcttc cgtacggcct ggagccgaca tccagcgcat tcttgagagc ggccggcacc
     9181 gaccggttat attcgggggt gacgaacaaa attgcatcgg cggccttgac gcgctggcgg
     9241 aatgcggtcc gtggcgcggg cgtagctgcg gtctccagat cctgattgaa aaacggcaat
     9301 tgcccgatct cgacggtttc gagcttcatc gaagatggtg cgagcgagat cagcgcattc
     9361 gccagcatcc cgttgagcga cgccttccgc aggcttccaa ccagcacgga cacattcatc
     9421 ggcttatcca cggtcatgcg gtcgccggag atttgacggt cctcgcgaaa tcgggcttgc
     9481 gcttttccag gaaagccgag aacgactctt ttgcgtcgtc ggagcggact tgcgcactga
     9541 actcctcgtt ctcagccttc atcgccgcct tgatctgctc gcgaaatggc tgcttcatga
     9601 gcctcttgct ggcttgcaac gctgcagcag gcttcgctgc cagtttccgg gccgtctcgg
     9661 tcgccctcgt cagcacatcc ttgtcggata cgacctcggt aaccagcccc aactccgcgg
     9721 cgtgcctggc atcaaacgga gctcccaaca aaagcagctc cgcggcacgg acatgaccga
     9781 tccgcgccgg tacggagcag ctcgatccga attccggcac gacagcaaga ttgatgaagg
     9841 gcatctggaa tttcgtgccc tcgcccgcgt agatgaagtc acagtgcgtc agcatggtgg
     9901 tgccgccgcc gatcgcggcg ccgtggaccg ccacgaccac tggtttgtcg aagttgacga
     9961 atgcgttcat gaggccggcc tgtggcgatt cgcctggccc tggaggattc ttgagaaagt
    10021 cctcgatatc gttgcccgcg cagaatgaat ctcccgcgcc atgccaaagc acgacacgcg
    10081 tgttcgcgtc attcgccgcc ttgttgaaga tgccggcgag agccgcgtac atggctgacg
    10141 tcatcgcatt ccgcttggtc gggcggttga actgcacgcg caggatgctt cccgcatctt
    10201 cggtaacgat atcatccatt gtcgaagtcc cggcgccttt ggtgaatttc gctgcaagtg
    10261 gaggttcggg gcggctcccg ccgccccggc ggggtcgtcg tgctaggccg catgttcctt
    10321 tggttttgga atggcaccgc tcgcacgcaa accatcgatt tcggtcgcgc tgaatccgag
    10381 ctgccggaga acctcgtcgt tgtgttctcc aatcttcggg gcgcgcttcg caggcacctt
    10441 ggcgacgcca tgtacctgaa gcgggctgct gatggtggat gtcaacttgc cgccggcacc
    10501 attgagcgga acgatgatgt cattcgcctg gagttgagga tcgttgatca cctcttgcgg
    10561 cccacgtacc gctccgaacg tcacgtgaac gccgttgaac atctcgtacc aatgcgccat
    10621 cggctcgccg cagaagatct cgtcgaggat tgccgtgagc tgcggcatat tcgctgtcag
    10681 cttcgccgga tcggaaaagc gcgggtcggt caggagatcg gcacgaccaa gggctttggc
    10741 gacggctgcc agcttgtcag gcgtgacgat gagaacgaac caggtgccat cctttgcgcg
    10801 atacacattc attgccgcat ttgcggggtg catacgatcg tgcagcccaa agaacttcgc
    10861 ttcgcaaagc gctgcctgga tcgagacgct ggcggaccag acgccttcgg cgagcagaga
    10921 ggtggtgaca tgcgctccct tcccggtgcg ctcgcgccga tagagagcgg tgacgatcgc
    10981 ggaatagaga ccgacggcgg tggcattgtc accgctgccc gccaccggcc aggtcggtgg
    11041 tgcgccggca tcgcgcgtca tcgacagcag accgctgcgg gcccaatatg acgtgatatc
    11101 gaagcccggt agatccgcat caggtccctt atcgccgaag cccgtgatgt cggcatagat
    11161 caaccgcgga ttccacggca tcacgtcctc gtagttgagc ttcagccgcg cgcgcgccgg
    11221 atgcggcgta ttgacgatga ggacatcggc ccatttgacc agcttttcga gaaccgcatg
    11281 ggcgcttggc gacttcagat caagcgccat accccgcttg ttgcgattgg cgaggtgcca
    11341 gggataagga tcctcggcga ccggttgcgg cggcatgttg tgcgcatgcc gccacaactc
    11401 gccgctcggc ggctccacct tgatgacgtc ggcgccgaag tccgacagga tcaccgcggc
    11461 gctggggccg gcgataaagc tcgccagatc caccactttc agtccagaga aaatattgtc
    11521 gcttgccatg gctgtccttt ccaaatcgtg gtgggggttc accgaccgcg gaacttcggt
    11581 gcacgcttct cgaggaaggc ggaggtgcct tccttcttgt cttccgttgc cgcgcagatg
    11641 ccgaaatagg acgcttcgag cgcaaacccc tcggtctggc tggtgtccat ccctttgttg
    11701 gccgcctgca gtgagaactt cacggcgatc ggcgcattgg ccatgatctg cttcaggatc
    11761 gtttcggcgc ggtcgatcag gccggcggca gaaacgactt cgttgacgag gccgatgcga
    11821 taggcttcct gcgcggaaat cgtctcgccg gtgaggatca gctgcaacgc acggcccttt
    11881 cccaccaggc gcggcaaacg ctgcgttccg ccacccccgg gcagcaggcc gagcttgacc
    11941 tcgggttgcc cgaatctggc atgctcggcc gcgatcctga tcgtgcaagc catcgccgtc
    12001 tcgcagccgc cgccgagcgc aaagccattg atcgccgcga tcaccggctt gccgagatct
    12061 tcgataaggt ccaacacccc ctgcccgaac cggctggact cctccgcgtc ataggcgtcg
    12121 acattcgcaa gttcactgat gtcggcgccg gcgatgaagg ccttgtcgcc cgcgccggta
    12181 aggatcacgc cgtgcacgga cgcgtcggcc tttgcatcct cgaatgcggc ccgcaaatca
    12241 gtccaggtcg gcgtattgag cgcgttgagc accttgggcc gattgaccgt cacataggcg
    12301 atcgaaccgt ttttctcgta caggacattc gcaagcgtca acgccgccct gggcaaggag
    12361 ttgggtactg ttgcaggcga aggggtgagc ggtgcagttc ccgaaaccat aatcatatct
    12421 cctggatggg gagggcagcc ggcgtcggag cgccggcgcc gctgggaagt cagatgaagg
    12481 cgccgtgacc ggtgatggcc ttgccgacaa tcagattttg catctgataa gtgccttcgt
    12541 aggagtacag cgcttcggcg tcggcgaaga agcgcgcgac attgtagtcg gcgacgatgc
    12601 cgttgccgcc caggacctcg cgtccccatg agacggtctc gcgcgatttc gccgtacaga
    12661 acgccttcgc cagcgccgca tgatggtcgc caagcttgcc ttcgtcatca agctgcgcca
    12721 ggcggagcat caggcactgg cacgcggtca gattggcaag catcttggcg agcagatcct
    12781 ggatcatctg gaacgaagcg atcggcttgc cgaattgctg tcgctcctgg gcatatttga
    12841 cggttgcctc gaatgcgccc atctggattc cggtcgacgc ccaacccacc atgtatcgc
//
LOCUS       DASWGM010000165         8552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1952800,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000165 DASWGM010000000
VERSION     DASWGM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952800"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VGA15_05005"
     CDS             <1..900
                     /locus_tag="VGA15_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9587085.1"
                     /translation="VVEQVGSDVNYVKPGDHVVTCLSVFCGTCDNCTTGRTVLCTDTT
                     VKMLPGASNRLSWARPEKLNQFLNLSSFAEQMLVHENAIVKIRKDMPLELAALIGCGV
                     ITGYGAVVNTAKVAPGETVVVIGCGGVGMAAINGAQIAGAGRIIAVDTNPAKLQLATK
                     LGATDIVDPQNGDVVQQVRDLTNGGVHHSFEVLGRKETAEQAFAMLAPGGTATIVGMI
                     PFGQKIELHGFDFLRERKIQGSSMGSNHFRVDMPRLIEFYMRGKLHLEDWISAKLKLS
                     EINEGFAAMKAGKTVRSVIVFDA"
     gene            complement(1017..1370)
                     /locus_tag="VGA15_05010"
     CDS             complement(1017..1370)
                     /locus_tag="VGA15_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9587086.1"
                     /translation="MSKDIDELTVLNRDYVDSVQNSDVKRFDEILAEDFYCSNPDKSL
                     VDRAGFLKQTAMPVTIKNLIADEVKIRVMGDFAIIHARTSYTTADGQPASGRYTDCWA
                     RQNGRWLAVSAHVSR"
     gene            complement(1562..3034)
                     /locus_tag="VGA15_05015"
     CDS             complement(1562..3034)
                     /locus_tag="VGA15_05015"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9587087.1"
                     /translation="MAKSEWSFETATELSAALAAKKVSAVELAQDAIGRIERHDGKIN
                     AICVRDFERGLDAARAADASRARGETRPLLGIPLTVKESYNIAGLPTTWGFPAQKDFV
                     PGEDALSISRVRGAGGVILGKTNIPVGLGDWQSYNDIYGTTNNPYDLGRTPGGSSGGS
                     AAALAAGYGPLSLGSDIGGSLRVPAFHCGVYAHKPTFALVPSRGHTPPPFPPLPFDRD
                     LAVIGPMARSAADLSLLLDVIAGPDPMEIGKAYTLALPPPRHGALKNFRVLVVDTDPV
                     LPTNAAVRAAIDRLAANLGKADVKIERQSPLLPDFAASSRLYMRMLMSFLGATFAPEI
                     YAGAHEAATKLAPDDISLAAERLRGIALSHRDWVLAEGGRARLRAQWRELFKSFDAVI
                     CPVMPTPAYPHDHSPEQETRRINIDGKDYVYPDQLAWPGIATLPGLPATAIPIGLSQE
                     GLPVGVQIVGPWLEDRTPLKLAELIEREFGGFVRPPAFDD"
     gene            3336..4436
                     /locus_tag="VGA15_05020"
     CDS             3336..4436
                     /locus_tag="VGA15_05020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9587088.1"
                     /translation="MGQLIETSSAATLDWRIVAPAEAGFAGDLEARLDKAIAEKRIWN
                     LHSVVIMRNGRLVLERYFDGQDDARGRPLGVVSFKPDTLHDLRSASKSIVGLLYGIAL
                     AAGKVPPPEQPLMRSFPEYADLFADPERSRWTVHHVLSMTLGTDWDESSIPYTDPANS
                     EIAMDRAPDRYRYVLGRPIVMEPGKRWTYNGGATALLGRIIANGTGKSLHEYAREVLF
                     EPLGMGPTDWFKGNDGELIAASGIRMTPRDLTKIGQLMLRGGVWDERVVVPAEWIARC
                     TSPVVPIDEVREYGYHWYVGRYAFETPTAPRWNRSRLERYWSAAGNGGQRLFVIPGLD
                     LLVAVTAGNYDTPDQWVPPARVVREVVLPAIL"
     gene            complement(4456..4986)
                     /locus_tag="VGA15_05025"
     CDS             complement(4456..4986)
                     /locus_tag="VGA15_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX9587089.1"
                     /translation="MTDRLQYGTTAKVFHWLLVALLLVQYLIGWLMPDIHRGVKPGAA
                     MTFHVSFGIVILALIVLRFAWRLTHPVALESSLPPWQRLSLEAVHWLLYALVLATTVT
                     GWLFASFRGWSLSFFYLVPMPMLASDNAAAGKTIDGLHQAMEWALLVLIGIHVASAFA
                     HIFIYRDRIMQRMLPG"
     gene            complement(5062..6033)
                     /locus_tag="VGA15_05030"
     CDS             complement(5062..6033)
                     /locus_tag="VGA15_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX9587090.1"
                     /translation="MKNFADLTEREVLAVAISSEEEDSRIYMTFAEDLAERYPESAKI
                     FEEMAEEEKGHRHKLLEMYEQRFGKNLPPIRRDNVRGFLRRRPIWLTKNLSLDAIRRE
                     VGTMEFEAERFYIKAAEQAQDVGVRRLLGDLAEAEKGHEKTAAKLTDQILSPDVRAEE
                     DKTRRRVFVLQYVQPGLAGLMDGSVSTLAPLFAAAFATHQNWQTFLVGLAASIGAGIS
                     MGFAEALSDDGSLTGRGSPWLRGVTCGAMTTVGGLGHTMPYLVPDSWANAFWIATAIA
                     GVVVFFELWAIAYIRARYMDTPFLQAVFQIVLGGAIVLAVGILIGAA"
     gene            6210..6932
                     /locus_tag="VGA15_05035"
     CDS             6210..6932
                     /locus_tag="VGA15_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587091.1"
                     /translation="MNKVKYRLNDPRLAPRRTKLEIPGWAGKREPRTDGSREQVWHCV
                     PFSEGAQYGIELFYPYDNELHVSTKDGQLILDGDWGPPPDGGVQWPPFRNFGDHFYTY
                     QILLDLKVEKGMAIRTEPHPRFYTDRTDTVPIAVPALVRNWWPMLFFTVFKAPAEGRT
                     HIFRPNEPFAQIIVIPEEAPFELEAMDEEESAERELQARRIYASRPTLSAGTEWTSST
                     DTVFDGTYRHLHRAAKEKARQD"
     gene            complement(6999..7394)
                     /locus_tag="VGA15_05040"
     CDS             complement(6999..7394)
                     /locus_tag="VGA15_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587092.1"
                     /translation="MEKKIAGLLGAMATLGAFNGAEAAPAPSPAPTDVLRANSFADLL
                     EPIPNAAALLRAVDESRPNSSADENVQLAQFYYHHHHHHHHHHHNFYRRYDYDEPRII
                     VVPPRYGRYYYRHHHHHHHHHHSFYRRDY"
     gene            complement(7519..8550)
                     /locus_tag="VGA15_05045"
     CDS             complement(7519..8550)
                     /locus_tag="VGA15_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587093.1"
                     /translation="MLLHGFPELAFSWRKVMPQLAAAGYHVIAPDQRGYGRTTGWDAN
                     YDGDLASFRLLNLVRDALGLVSAFGYRSVDTVVGHDFGSGVAAWCALVRPDVFRSAVL
                     MSAPFAGPPQFAFSTADAPPRPRPDDPVHRELAALPRPRKHYQWYYSTREANADMHRA
                     PQGVHDFLRAYYHHKSADWKDNKPYPLASWSANELAKLPTYYVMDLARDMAGTVAEEM
                     PDPAAIAANTWLPDRELSFYSAEYARTGFQGGLQWYRCGTSGTFTAELETYSGRSIDV
                     PACFISGKQDWGTYQRPGVFEAMQASACTRMFGCHLVDGAGHWVQQEQPEKVTHLLLQ
                     FLQQAKAPR"
BASE COUNT         1665 a         2607 c         2668 g         1612 t
ORIGIN      
        1 gtcgtcgagc aggtcggctc cgacgtcaac tacgtaaaac ccggcgatca tgtcgtgact
       61 tgtttgtcgg tgttctgcgg cacctgcgac aactgcacca ccggccgcac ggtgctgtgc
      121 accgacacga ccgtgaaaat gctgccgggc gcgtccaacc ggctgtcatg ggcgcgaccg
      181 gagaaactca atcagttcct caacctgtcg tcgttcgccg agcagatgct ggtgcacgaa
      241 aacgccatcg tcaaaatccg caaggacatg ccgcttgaat tggcggcgct gatcggctgc
      301 ggcgtcatca ccggctacgg cgctgtggtg aataccgcga aggttgcgcc cggcgagacc
      361 gtggtggtga tcggctgcgg cggcgtcggc atggccgcga tcaacggtgc gcagatcgcc
      421 ggcgccggcc gcatcatcgc ggtcgacacc aatccggcca agcttcaact ggcgacaaaa
      481 ctcggcgcca ccgacattgt cgatccccaa aacggcgacg tggtgcagca ggtgcgcgat
      541 ctcaccaatg gcggcgtgca tcactcgttc gaagtgctgg gccgcaagga aaccgcggag
      601 caggcgtttg cgatgctggc gcccggcggc accgcgacca tcgtcggcat gattccgttc
      661 ggccagaaga tcgagctgca cggttttgac ttcttgcgcg aacgcaagat ccaggggtcg
      721 tcgatgggct cgaaccattt tcgcgtcgat atgccgcggc tcatcgaatt ctacatgcgc
      781 ggcaagctgc atctggagga ctggatttcg gcgaagctaa aactgtccga gatcaacgaa
      841 ggttttgccg cgatgaaggc cggcaagacg gtgcgcagcg tgatcgtgtt tgatgcttga
      901 attccaccgc cgtcatgcgc ggacttgatc cgcgcatcca ttttcttcgc aagaactttt
      961 ttcaaaggga tggattgccg ggtcaagccc ggcaatgaca gcctgtgaga tcatgctcac
     1021 cgcgagacat gtgctgatac cgcgagccag cggccatttt gccgcgccca gcaatcggtg
     1081 tagcggcctg atgccggttg accgtccgcc gtcgtgtagc tggtgcgggc gtggatgatg
     1141 gcaaaatcgc ccatcactcg aatcttgacc tcgtctgcga tgagattctt gatcgttact
     1201 ggcatggccg tctgcttgag aaatccggcg cggtcgacca atgacttgtc gggattggag
     1261 caatagaaat cctcggcgag gatttcgtcg aaacgcttga cgtccgagtt ctgtaccgag
     1321 tcaacatagt cgcgattgag caccgtcagt tcgtcgatat ccttgctcat cgtctgtcct
     1381 ttctctgacc gtcattccgg gatgcgccac cgaactcggg tttacccgag ttcggcaata
     1441 tcaatgtcca agtcggcaac agccgacttg gatgcgcagg cccggaatcc atactcccgg
     1501 cgggggttat ggattccggg ctcgtttcgc ctcgctcacg ccccggaatg acgaactgaa
     1561 ttcagtcatc aaacgccggc ggccgtacaa aaccgccgaa ttcgcgctcg atcagttcgg
     1621 ccaattttag cggcgtgcga tcttccagcc aggggccgac gatttgcacg ccgaccggca
     1681 gtccttcctg cgaaagcccg attggtatgg cggtggccgg caggccgggc agggtcgcga
     1741 tgccgggcca tgccagctga tcggggtaga catagtcctt gccgtcgatg ttgattcgac
     1801 gcgtttcctg ctcgggcgaa tgatcgtgcg gataggccgg cgtcggcatc acagggcaga
     1861 tcaccgcgtc gaaactcttg aacagctcgc gccactgcgc cctcaagcgc gcgcgcccgc
     1921 cttcggccag cacccagtcg cggtggctca gcgcgatgcc gcgcagtcgc tcggcggcga
     1981 ggctgatatc gtcaggcgcg agctttgtcg ccgcttcgtg cgcaccggca tagatctccg
     2041 gcgcaaacgt cgcgccgaga aacgacatca gcatccgcat gtagagccgc gacgacgccg
     2101 cgaagtccgg caacagcgga ctttggcgtt cgatcttaac gtcggctttg ccgaggttcg
     2161 cggcaagtct gtcgatggca gcgcgcaccg ctgcattggt cggcagcacc gggtcggtgt
     2221 cgaccacgag cacgcgaaag ttcttcaacg cgccgtgacg cggcggcggc agcgcgagcg
     2281 tgtaggcttt gccgatctcc atcgggtcgg ggcctgcaat cacgtcgagc aggagcgaaa
     2341 ggtccgcggc actgcgcgcc atcggcccga tcaccgcgag gtcgcggtcg aacggcaatg
     2401 gcggaaacgg cggcggcgta tggccgcgcg acggcaccag cgcgaaggtc ggcttgtgcg
     2461 catagacgcc gcaatgaaac gcgggcacgc gtagcgagcc gccgatgtcg gagccgagcg
     2521 agagcggccc gtaccccgcc gcgagcgccg ccgccgagcc gccggaggag ccgcccggcg
     2581 tgcggccgag atcatacgga ttgttggtgg tgccgtagat gtcgttatag ctctgccagt
     2641 caccgagccc gaccggaatg ttggttttgc cgaggatcac gccgcccgca cctctgacgc
     2701 gggaaatcga cagcgcgtct tcgcccggca caaaatcctt ctgcgccgga aagccccagg
     2761 tcgtgggcaa ccctgcgata ttgtaggatt ccttcaccgt gaggggaatg ccgagcagtg
     2821 gtcttgtctc gccgcgcgcg cggcttgcgt cggcggcgcg ggcggcatcg aggccgcgtt
     2881 cgaaatcccg gacgcaaatc gcgttgatct tgccgtcgtg ccgctcgatg cggccaatcg
     2941 catcctgcgc cagttccacg gcagagactt ttttcgccgc gagggctgcc gataattcgg
     3001 tcgccgtctc aaaactccat tccgacttgg ccacggcgta ctcctgctct tcgttgttcc
     3061 ggttgcacga gatgatgctc cgttccgacg agggccgcta gacagctatt gctgccagag
     3121 gacagctatc ccggcagcaa gcactgccga taattcgatc gccgtcttga aaccccattc
     3181 cgacttggcc aaagtatgct cccggtctag tttctgatca ccggatgatg ctcagtttgg
     3241 gcgcgggccg caagaacgac gcggggagaa tcgcaaacgt gaatgagcca acacttagcc
     3301 gtcgacaaat cctggcggcc gcaggaggaa tattcatggg ccaattgatc gagacaagtt
     3361 ccgccgcaac gctcgactgg cgcatcgtcg cgccggccga ggccggtttc gccggcgatc
     3421 tggaggcgcg gctcgacaag gcgatcgccg agaagcggat atggaatctt cacagtgtcg
     3481 tcatcatgcg caacggacgg ctggtgctgg agcgctattt cgacggacag gatgacgcgc
     3541 gcggccggcc gttaggcgtg gtttcgttca aacccgatac gcttcatgat ctgcgctcgg
     3601 cgtccaaaag catcgtcggc ctgctgtatg ggatcgcgct ggccgccggc aaggtgccgc
     3661 caccggaaca gccgctgatg cgttcgtttc ccgaatatgc cgatctcttt gctgaccccg
     3721 agcgcagccg ctggacggtc catcacgtgc tttcgatgac gttgggcacc gattgggacg
     3781 agtcgagtat tccctatacc gatccggcca acagcgagat cgcgatggat agggcgcccg
     3841 atcgctatcg ctacgttctc ggccggccca tcgtgatgga gccgggtaaa cgctggactt
     3901 ataacggcgg cgccaccgcg ctgctggggc gcatcatcgc caacggcacc ggcaaatccc
     3961 tgcatgagta tgcgcgcgaa gttctgttcg agccgctcgg catgggtccg accgattggt
     4021 tcaagggaaa tgacggcgaa ctgatcgccg catccggcat ccgcatgacg ccgcgcgatc
     4081 tgaccaagat cggtcaattg atgctgcgcg gcggcgtttg ggacgaacgt gttgtcgtgc
     4141 cggcagagtg gatcgcgcgc tgcacgtcgc ccgtggtgcc gatcgatgaa gtgcgcgaat
     4201 acggctatca ctggtacgtc gggcgttatg ccttcgagac accaaccgcg ccgcgctgga
     4261 acaggtcgcg gctggagcgt tactggagcg ccgccggcaa cggcggccaa cgcctgttcg
     4321 taattcccgg gctggacctg ctggtggcgg tgacggccgg taactacgac acgccggatc
     4381 agtgggttcc gccggcgcgc gttgtccgcg aagtcgtgct tccggccatc ttgtgagctg
     4441 tgtcgcccgg gaagcctatc ccggcagcat ccgctgcatg atgcgatcgc ggtagatgaa
     4501 gatgtgggcg aacgccgacg ccacatggat gccgatcagc accaagagtg cccactccat
     4561 cgcctgatgc aggccgtcga ttgtcttccc cgccgcggca ttgtccgagg ccagcatcgg
     4621 catcggcacc agataaaaga aggacagcga ccagccgcga aacgaggcga acagccagcc
     4681 ggttacggtc gtcgccaata ccagcgcata gagcagccaa tgcacggctt ccaagctcag
     4741 ccgctgccac ggcggaagcg aactctccag cgcgaccgga tgggtaaggc gccaggcgaa
     4801 tctgagaacg atgagcgcga ggatgacaat tccaaaggag acatgaaacg tcatggcggc
     4861 gccgggtttc acgccgcgat gaatgtccgg catcaaccag ccgatcagat attgcacgag
     4921 cagcagcgcc accaggagcc agtggaacac cttggcggtg gtcccgtatt gcaaacggtc
     4981 ggtcatgatt ttccttatgc cagagccaga acattttttg gcataatgga aaccgagttg
     5041 ccgttagaaa cgctctaatg cctacgccgc gccgatgaga atgccgaccg cgagtacgat
     5101 ggcgccgccg agcacgatct ggaataccgc ctgcaggaac ggcgtgtcca tgtaacgcgc
     5161 gcggatgtag gcgatcgccc acaattcgaa aaacaccacg acgccagcaa tggcggtcgc
     5221 gatccagaat gcgttggccc aggaatccgg aaccagatag ggcatggtgt ggccgagacc
     5281 gccgaccgtc gtcatggcgc cgcaggtcac accgcgcagc cagggcgagc cgcgcccggt
     5341 cagcgatccg tcgtcggaaa gcgcttctgc aaaacccatg ctgatgccgg ccccgatcga
     5401 ggcggcaaga ccaaccagga acgtctgcca gttctgatgg gtcgcaaaag ccgcggcgaa
     5461 cagcggcgcc agcgtcgaca ccgacccgtc catcagtccg gccagacccg gctgcacata
     5521 ttgcagcacg aatacacgcc ggcgcgtctt gtcctcttcc gcgcgcacgt ccggactgag
     5581 gatctggtcg gtcagcttgg ccgcggtttt ttcgtggcct ttttcggctt cggcgagatc
     5641 gccgagcagc cggcggaccc cgacatcctg cgcctgttcg gcagccttga tgtagaagcg
     5701 ttcggcttcg aactccatcg tcccgacttc ccggcggatg gcgtcgagcg acaggttttt
     5761 tgtcagccaa atggggcggc ggcgcaaaaa ccccctgacg ttatctctgc gaatgggcgg
     5821 caggttcttg ccgaaacgct gctcgtacat ttccagcagc ttgtggcgat ggcccttttc
     5881 ctcctcggcc atttcctcga agatcttggc cgattccgga taacgctcgg cgaggtcttc
     5941 tgcgaaggtc atatagatgc ggctgtcctc ttcctccgaa gagatcgcca ccgccaggac
     6001 ttctcgctcg gtcaaatcgg cgaaattctt cacaacagcg cctctttcca gtttagaatg
     6061 attctaaaga cgttatagcc acgcgttgtt gggaggtcaa atcattcggc cgttcagctg
     6121 cgcgtgcgtt cagggcctca caatctggaa ctgttgcggt cggcgtccgt tcgagttcct
     6181 atcgcaagat ccatcgaccc ggagtgctca tgaacaaggt caaataccgc ttgaatgatc
     6241 cgcggctggc gccccgccgc accaagctcg agattcccgg atgggcgggc aagcgcgaac
     6301 ctcgcacgga cggctcgcgc gaacaggtgt ggcattgcgt tccgttctcg gaaggcgcgc
     6361 aatacgggat cgaacttttt tacccctacg acaatgaact gcacgtcagt accaaagacg
     6421 gccaattgat actcgacggc gattgggggc caccgccgga cggcggcgtg caatggccgc
     6481 cattccggaa ttttggcgac catttttaca cctaccagat ccttctggat cttaaggttg
     6541 aaaagggcat ggcgatccgg accgaaccgc acccgcggtt ctataccgac cgcaccgaca
     6601 ccgtgccgat cgcggttccg gcgctggtgc ggaattggtg gccaatgctg ttcttcacgg
     6661 tgttcaaggc gcccgccgag ggccgtaccc acattttcag gccgaacgaa ccgttcgcgc
     6721 agattatcgt gattcccgag gaagctccgt tcgaacttga ggcgatggac gaagaggaat
     6781 ccgccgagcg cgaactgcag gcgcgccgca tctatgccag ccgaccgacg ctgtcggccg
     6841 ggaccgagtg gacctcgtcg accgacaccg tgttcgacgg gacctatcgg catcttcacc
     6901 gtgcggccaa ggaaaaggcc cgccaggatt aagccgcaca aaaaaacagg accctgaggt
     6961 cctgcttgtt cgtcatcccg cggggggcgc gcgcgacctc aatagtcgcg gcggtagaag
     7021 ctgtgatgat ggtgatggtg gtggtgatga tgacggtaat aataacgacc gtatcgggga
     7081 ggcaccacga tgatgcgggg ctcatcataa tcatatcggc ggtagaagtt gtgatggtga
     7141 tgatgatggt ggtggtgatg gtggtaatag aattgagcaa gctggacatt ttcgtccgct
     7201 gacgagttcg gccgtgattc atcgacagcc ctgagcagcg cagcggcgtt cgggatcggc
     7261 tcgagcaggt cggcgaaaga attggcgcgc aaaacgtcgg taggggccgg acttggagcg
     7321 ggcgcggctt cggcgccgtt gaacgctccg agcgtcgcca tcgctcctaa aaggccagcg
     7381 atcttctttt ccataaaact cctcctccag tgactatcgt cgggcgcacc acccggtgat
     7441 cggcaacgcg gcccctggga aatggttccc gcgacgattt cgcgataatg tcagaccgat
     7501 agtgaaccga ggatgaactt agcggggtgc tttggcctgt tgcaggaatt gcaacagcaa
     7561 gtgagtaacc ttctcgggct gctcctgctg cacccagtgg ccggcgccgt cgaccagatg
     7621 acagccgaac atgcgcgtgc acgcgcttgc ctgcatcgct tcaaacacgc cggggcgttg
     7681 ataagtgccc cagtcctgtt tgcccgagat aaagcaagcg ggcacgtcta tactacgacc
     7741 tgaatatgtt tcaagttcgg cggtgaatgt gcccgaggtg ccgcagcgat accattgcag
     7801 tccgccctgg aatccggtgc gggcatattc ggcgctgtag aaggaaagct cgcggtcggg
     7861 cagccatgtg ttggctgcga tcgcagccgg atcaggcatt tcctctgcga cggtcccggc
     7921 catgtccctg gcaagatcca tcacatagta agtcggcagc tttgccaact cgttcgcaga
     7981 ccacgatgcg agcggataag gcttgttgtc tttccagtcc gcactcttgt gatgataata
     8041 ggcgcgcagg aaatcatgca cgccctgtgg cgcgcggtgc atgtcggcgt tggcctcgcg
     8101 cgtcgagtag taccattgat agtgcttgcg cggtcgcggc agcgccgcaa gctcgcgatg
     8161 cacgggatca tccggccttg gccttggcgg tgcatccgcg gtgctgaagg cgaactgtgg
     8221 cggtcccgcg aacggcgcgc tcatcagcac cgccgacctg aacacgtcgg gccgcaccag
     8281 cgcgcaccat gccgcaaccc cgcttccgaa atcgtgaccg accaccgtat ccacgctgcg
     8341 atagccgaac gccgacacga ggccgagcgc gtcgcgcacc agattgagca gccggaacga
     8401 ggcaagatca ccatcgtaat tcgcgtccca gccggtggtg cggccatagc cgcgctgatc
     8461 cggcgcgatc acgtgatagc ccgcggctgc aagctgaggc atcaccttgc gccagctaaa
     8521 ggccagttcg ggaaagccgt gcagcagcag ca
//
LOCUS       DASWGM010000166         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2233482,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000166 DASWGM010000000
VERSION     DASWGM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233482"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGA15_05050"
     CDS             <1..203
                     /locus_tag="VGA15_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587094.1"
                     /translation="ILDHRERIGSGVDDAFEEGHAVSALFFLKEHTMVDYARSRPTRA
                     GLTHRRRLQTPDRSSPGSAGIK"
     regulatory      595..747
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            816..1412
                     /locus_tag="VGA15_05055"
     CDS             816..1412
                     /locus_tag="VGA15_05055"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX9587095.1"
                     /translation="MNQMLQDAPQVQDAPEVDAPEVNDSPAPETVDRPPVPAHPRFAK
                     PQRVAFVQSAWHREVVQECRLAFLEEIEARHIARAQIDLFEVPGSFEIPLHAQLLAKT
                     RRYTAIVAAGLVVDGGIYRHEFVADTVIKALMDVQLRTEVPVFSAVLTPQQFHETAAH
                     FEFFRKHFATKGIEVAEACANTLLSLERLRGQVAAGIV"
     gene            1513..2772
                     /locus_tag="VGA15_05060"
     CDS             1513..2772
                     /locus_tag="VGA15_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEX9587096.1"
                     /translation="MFEGHDPYLVALSVVIAILGGYTGFGLAARIRGTPGVSRRVLLA
                     GAAAFLAVGIWTMHFVGMLAAPIPADTVYLVLPTIVSFLICALVVGISLFFVSIGEPS
                     LRRVISSAVLLGAGIASMHYVGIHGLVGDFAITHDNAMIFLSVLVAIVAAYGGLRAFL
                     ARQDGVRLIVSSIAFGVAVSGMHYTAMAGMHFAALSGEAHHHGGGLAASQQILSVVVA
                     LLCFVIAAGFLLSLVPDPRRQPVATAAVTTGPVAEAAGTTPPSSPEPVPVAASMPRPM
                     SAPLGGLGQPPRAAPAPRLPVEGANGTHFIDTADIRSVRADAHYTRVHDGTRERMCPW
                     SISEAEAQLDPGLFVRVHRSHIVAIPHVTFVRREGDGAIVELDGPSPHRVPVSRAKIA
                     EVKARLGLARRNAQPVQTGLGRQGRQD"
     gene            2914..3777
                     /locus_tag="VGA15_05065"
     CDS             2914..3777
                     /locus_tag="VGA15_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9587097.1"
                     /translation="MIPGPFSYHRPATVADAVKLLSTLGDEARPLAGGHSLVPMMKLR
                     LATPEHLVDLHGIDGLKGIRREAGRIVIGAMTTQHELLASDDIAKSLPILHETALLIA
                     DPQVRYMGTIGGNVANGDPGNDMPALMLTLGASYRLEGPSGGRDIAASEFYQGAYFTA
                     LEPGEILTFISIPAPAAGHGYAYEKLKRKVGDYATAAAAVVLTMAGGKVASCAIGLTN
                     LHETPLLAADAAKLVIGTSLDAATLKKAAAAAEAIMSPAADARGPVEYRKHVGGIMVM
                     RALTRAASRAS"
     gene            3793..>4218
                     /locus_tag="VGA15_05070"
     CDS             3793..>4218
                     /locus_tag="VGA15_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9587098.1"
                     /translation="MAKTHVTMKVNGAEVEGLVEPRTLLVHFIRENLLLTGTHIGCET
                     THCGACTVDLDGMSVKSCTMFAVQAQGCEITTIEGVANADGSLSALQEGFRMMHGLQC
                     GFCTPGMILRAQRLLQENPSPTEAEIRMGISGNICRCTGY"
BASE COUNT          726 a         1351 c         1340 g          801 t
ORIGIN      
        1 atatcctcga ccaccgcgag cggattggtt cgggagtcga tgatgccttc gaggagggac
       61 atgccgtctc ggccttattt ttcttgaaag aacatacgat ggttgattac gcacgcagca
      121 ggccgacacg cgctggcttg acgcatcgtc gccgactgca aacccctgat cgatcgtccc
      181 cggggtctgc cgggatcaag tgatgtgatt tgcggaatcg gcgcaaccgc acggggagtc
      241 cgtccacagc caaagcggtg ctctgtccac agctgatcgc gcggcataat tattaatttg
      301 agaacaaacc ggaatcggat tcgcgcaggc cttgtccggt gcggcgaatt catgtccggc
      361 gagtcggttg gctcgcctta atcgttaatt ttcttgttga tttttctcag ggccggcccg
      421 gcctggaggc tagacatagt aagcctagcc tcaccaagcc tagacatagt gagcctagcc
      481 atagtgagcc tagccttggc gtcatggcga catgcggatt ccccatggtg aaggtgcttg
      541 ctgacgtctc agcctttcgt catagttccc cttaggccgc tcatgctggg cggccgatgt
      601 tctcagggcg gggtgaaagt ccccaccggc ggtaagggcg aagaagccca agcccgcgag
      661 cgccttcttc ccttgtggga aaaagggtca gcagattcgg tgtgattccg aagccgacgg
      721 ttaaagtccg gatgaaagag aacggtcgcg acagccgcat tccgcgcggc gcgtcgttcc
      781 gtgtgccctg attctggtcc tgaaagagga aagccatgaa tcagatgttg caagacgctc
      841 cccaagtaca agacgctccc gaagtagatg ctccggaagt aaacgactct cccgctcccg
      901 aaacggtcga tcgtccaccg gttccagcgc acccgcgctt tgcaaagccg cagcgggtcg
      961 ccttcgtgca gtccgcctgg catcgcgagg tggtccagga atgccggctc gccttcctcg
     1021 aggagatcga ggcgcgccat attgcgcgcg cgcagatcga tttgttcgag gtgcccggct
     1081 cgttcgagat ccccctgcac gcccaactgc tggccaagac ccggcgctac accgcgatcg
     1141 tcgcggcggg tctcgtggtc gatggcggca tctaccggca cgaatttgtc gccgacaccg
     1201 tgatcaaggc gctgatggat gtgcagttgc gcaccgaggt gccggtgttc tccgcggtgc
     1261 tgacgccgca gcagttccat gagacggcgg cgcatttcga attcttccgc aagcattttg
     1321 cgaccaaggg gatcgaggtc gcggaagcct gcgccaacac gctgctcagc ctggaacggc
     1381 tgcgcggtca ggtcgccgcg gggattgtgt aagtaacgca agcctggacg gattgattgt
     1441 catttccgca gccgcctata aactcggcgg cgggaggagg cacgcccgtg gagacgggcg
     1501 gccgagacga tcatgttcga aggacacgat ccctatctcg tcgcgctctc ggtggtgatc
     1561 gcgatcctgg gtggttacac cggtttcggc ctcgcggcgc gtatccgcgg gacgccgggc
     1621 gtgagccgtc gcgtgttgct cgcgggtgcg gcggcgttcc tcgcggtcgg catctggacc
     1681 atgcattttg tcggcatgct ggccgcgcct ataccggcgg ataccgtcta cctcgttctt
     1741 cccaccatcg tatcgttcct gatctgcgcg ctggtggtcg gcatatcctt gtttttcgtc
     1801 agcattggcg aaccctcgct gcggcgtgtg atctcttcgg cggtgctgct cggggcgggc
     1861 atcgccagca tgcattatgt cggcatccac ggtctcgtcg gcgatttcgc catcacgcat
     1921 gataatgcga tgattttcct ctcggtgctg gtcgcgatcg tcgccgccta tggcggcttg
     1981 cgcgcgttcc tggcccggca ggacggcgtc cggctgatcg tgagctcgat cgcgttcggc
     2041 gtcgcggtat cgggcatgca ctacaccgcg atggccggca tgcatttcgc cgcgctgtcg
     2101 ggggaggcgc atcaccatgg cggcggactg gccgcatcgc agcaaatcct ctcggtggtg
     2161 gtggcgctgc tgtgttttgt cattgccgca gggtttctgc tgtcgctggt gccggatccg
     2221 cggcggcagc cggtggcgac cgcggccgtc acgaccggtc cggtggcgga agccgccggt
     2281 accacgccgc cgtcatcgcc tgaaccggtt ccggttgccg catccatgcc gcggccgatg
     2341 tctgcgccgc tcggcggtct gggccagccg ccccgcgccg cgcctgcgcc ccggcttccg
     2401 gtcgagggcg ccaacggcac gcacttcatc gataccgccg atatcaggag cgtccgcgcc
     2461 gacgcccatt atacccgggt ccatgacggc actcgggagc ggatgtgccc gtggtcgatt
     2521 tcggaagccg aagcccagct cgatcccggc ctgttcgtcc gggtgcaccg cagccatatt
     2581 gtggcgatcc cccatgtcac cttcgtccgc agggagggcg acggcgcgat cgtcgagctc
     2641 gacggcccat caccgcatcg cgtgccggtc agccgcgcca agatcgccga ggtcaaggcc
     2701 cggcttggtc tcgccagacg caacgcgcag ccggttcaaa ccggattggg tcgccaaggt
     2761 cgccaggact gacgcatttc gtgcgggcga agccgcgttt cgtgcgaatt ccctcacctt
     2821 gtgaccggac gaccggcttc gccttgcctg cgaaaaaatc cggggacagc ctccgctcaa
     2881 cgtctgcaca caagaagacg catggaggag acgatgattc ctggcccatt cagctatcac
     2941 cggcccgcga ccgtggccga tgcggtcaaa ttactgtcaa ccctgggtga cgaagcccgt
     3001 ccccttgccg gcggccatag cctggtcccg atgatgaagc ttcggctcgc cacgccggaa
     3061 catctggtcg atctgcatgg catcgacggg ctgaaaggca tccgccgcga ggcgggtcgc
     3121 atcgtcatcg gcgcgatgac cacccagcac gaactgctgg cgtccgacga catcgcgaaa
     3181 tcgctgccga tcctgcacga gaccgcgttg ttgatcgccg atccgcaggt gcgttacatg
     3241 ggcaccatcg gcggcaacgt cgccaatggc gatcccggca acgacatgcc ggcgctgatg
     3301 ctgacgctcg gtgcgagcta ccgcctcgaa ggcccttccg gcgggcgcga catcgcggct
     3361 tccgagttct atcagggcgc ctatttcacc gccctcgagc ccggcgaaat cctcaccttc
     3421 atttccattc cggcgccggc cgccggtcac ggctacgcct acgaaaaact caagcgcaag
     3481 gtcggcgatt acgccaccgc ggcggcggcg gtcgtgctga cgatggcggg cggcaaggtc
     3541 gcaagctgcg cgatcggtct caccaatttg cacgagacac cgctgctggc tgccgacgcg
     3601 gcgaagctcg tgatcggcac cagcctcgac gcggcgacgc tgaaaaaggc cgcggctgct
     3661 gccgaagcca tcatgtcgcc ggccgcggac gcccgcgggc cggttgaata ccgcaagcat
     3721 gtcggcggca tcatggtgat gcgggcgctg acccgcgccg ccagccgcgc tagctgagaa
     3781 agggatcaaa aaatggcaaa gacccatgtg accatgaaag tgaacggcgc cgaggtcgaa
     3841 ggcctcgtcg aaccgcgcac gctgctggtg catttcatcc gcgaaaacct gctgctcacc
     3901 ggcacccata tcggctgcga gaccacccat tgcggcgcct gtaccgtcga tctcgacggc
     3961 atgtcggtga aaagctgcac gatgttcgcg gtgcaggccc agggctgcga gatcaccacc
     4021 atcgagggcg tcgccaatgc cgacgggtcg ctgtcggcgt tgcaggaagg ctttcgcatg
     4081 atgcacgggc tgcaatgcgg cttctgcacg cccggcatga ttttgcgcgc gcagcggctg
     4141 ctgcaggaaa atccctcgcc aacggaagcg gaaatccgga tgggcatttc cggcaatatc
     4201 tgccgctgca ccggctat
//
LOCUS       DASWGM010000167         5378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_704481,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000167 DASWGM010000000
VERSION     DASWGM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704481"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VGA15_05075"
     CDS             complement(<1..992)
                     /locus_tag="VGA15_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family competence protein"
                     /protein_id="HEX9587099.1"
                     /translation="MAERGGSSGRTQGYAGTWPPRTAAQAGGFAPSGFGAWSSFIERL
                     RAWIRAEAGAGRLLPWVPVAFGTGIAFYFAADREPVASVTAVAATAFGVAAFLLRRQK
                     IFPIAVMIAAMAAGFATATWKTARIAHGVLARPMYSVSLSGFVEARDIRERTDRFVLR
                     VSQMQSPRGQTTLERVRLSVRKGTAPAVGSFVELKARLQPPLAPLRPGSYDFGRDMYF
                     QGIGASGFVTGAIKTVEQPPLSGGLSLRYAALMQDLRDAIDARIRNVLDGDRRAIATA
                     LLTGRRDAITTPVNDAMFISGLGHVLSISGYHMAVVAGVVFFAVRALLALIPALT"
     gene            complement(1391..1537)
                     /locus_tag="VGA15_05080"
     CDS             complement(1391..1537)
                     /locus_tag="VGA15_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587100.1"
                     /translation="MKQSDLDSMSINELLMLHQRITATLDAKITAEKAALVDRLKQAD
                     MSVH"
     gene            2015..2206
                     /locus_tag="VGA15_05085"
     CDS             2015..2206
                     /locus_tag="VGA15_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587101.1"
                     /translation="MTLRDAADYIMKLPKAEQDLKEWQIAVACLIGAAATEIAHDQRA
                     PLLLPDEGQSAEACPIASS"
     gene            2242..2409
                     /locus_tag="VGA15_05090"
     CDS             2242..2409
                     /locus_tag="VGA15_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587102.1"
                     /translation="MARSPRPSTDAKTLNSSIRAKLAAASTKGSSRVQASHQPKPISM
                     PRLPWHDAEQR"
     gene            3145..3465
                     /locus_tag="VGA15_05095"
     CDS             3145..3465
                     /locus_tag="VGA15_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587103.1"
                     /translation="MPRRIMLSVFTIAVTAVTAGAADLPTQSRLGAIFAEPTEVRTRA
                     DRSRERVAPIMPFNLLPNPPWNRGGYYYGSSWSYYDGGPYYGGPLVFSGGRLPYVCGL
                     YGYC"
     gene            complement(4395..>5378)
                     /locus_tag="VGA15_05100"
     CDS             complement(4395..>5378)
                     /locus_tag="VGA15_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX9587104.1"
                     /translation="TLEFEDHRPLYEWFLDHLPVPSRPRQYESARLNITYTVLSKRVL
                     TELVRQRHVTGWDDPRMPTLAGLRRRGVPPAAIRDFIRRIGVAKANSVVDVGMLEFCI
                     RELLNKTALRRMAVLRPLKVVIENYPEGQTEEIEAVNHPDDPTAGTRRIAFGRELHIE
                     RDDFMENPPKKFFRLSPGTEVRLRYAYFIKCTDVIKNAAGEVVELRCTYDPATRGGNA
                     PDGRKVKATMHWLSAAQSVPAEIRIYNPLFAKPNPDAANFAADLNPQSLEILKDARIE
                     PAIAATNSNDAMQFERQGYFVRDPDSTADRLVFNRTIGLRDTFAKEVGGKG"
BASE COUNT         1103 a         1654 c         1642 g          979 t
ORIGIN      
        1 gtcaacgccg ggatcagcgc cagcagcgcg cgcaccgcga agaacaccac gccggcgacc
       61 accgccatgt ggtacccgga aatcgacagc acgtgaccga ggccggagat gaacatggca
      121 tcgttgaccg gcgtcgtgat cgcgtcgcgg cggccggtca atagcgcggt ggcgatggcg
      181 cgccggtcgc catcaaggac gttgcgtatt cgcgcgtcga tcgcatcgcg caagtcttgc
      241 atcaatgcgg cgtagcgcag cgacagcccg ccggagagcg gcggttgttc cacggtcttg
      301 atcgcgccgg tgacaaaacc ggacgcgccg atgccctgaa aatacatgtc gcggccgaaa
      361 tcgtaactgc ctggccgcaa cggcgcgagc ggcggctgca gccgcgcctt caattccaca
      421 aaactgccga ccgctggtgc tgtgcctttg cgcaccgaca gccgaacccg ctccagtgtt
      481 gtctgtccgc gcgggctttg catttgcgaa acccgcaaga caaaacgatc ggtgcgctcg
      541 cggatgtcgc gggcttcgac gaagccggac aacgacaccg aatacatcgg ccgtgccagc
      601 acgccatgcg cgatccgcgc ggttttccag gtcgctgtgg caaaacccgc ggccatggcg
      661 gcgatcatca cagcgatcgg aaatattttt tgccgccgca gcaagaacgc cgccacgcca
      721 aacgcggtcg cggcgaccgc ggtaaccgac gccaccggct cgcgatcggc ggcaaaatag
      781 aacgcgatgc cggtgccgaa ggccaccggc acccacggca acagccggcc ggcgccggct
      841 tcggcgcgga tccacgcacg cagtctctcg atgaaagacg accacgcccc gaaaccagat
      901 ggcgcaaaac caccagcctg cgccgcggtg cgcggcggcc aggtgcccgc atagccctgt
      961 gtccggccgg aagacccgcc ccgctctgcc atccggctgt accctacgca acgtcggagg
     1021 tcgtatgcta ccgtaaggtg tcacgcggcg aatagcggaa cggatgcaga atatcgggga
     1081 aagcgccgct gctcggcggc ttgtgccgcg ctttgatggg cgatgccatg gacaatcccg
     1141 aaccttttgc ttcacgacaa gcccgtgccg ctggccaaca tgcgaagtca cggtcgaaac
     1201 agggtgttcg tctgttgcag caatccgggc tgtcatcaca acgccgaatt gggggcgtca
     1261 gccgtcttcc ggatgacgtg accttcaacg acctacagcc acgcatggtc tgcacagtgt
     1321 gcgatcatcg tggcgccgat gtcagcccgt catggctgca tcatggctgg tcccggagat
     1381 cgcggacccg ctagtgcacg ctcatgtcgg cctgcttcag gcgatctaca agcgctgcct
     1441 tctcggcagt gatctttgcg tccagcgttg cagtgattcg ttggtggagc atcaacagct
     1501 cgttgataga cattgaatca aggtcgcttt gcttcatgac agcttccgat cacggaccac
     1561 tatcatgagc agaaaacgcc gacgattaaa aggaacactt cgaggcacgg ggtgttctgc
     1621 gcaacgaaca cacccgtatt tttacggaat tttcaaaggt gacatgtgca cgctaaggtg
     1681 ctcacgctga cccacgtcca ggggccctac caatggagtg tcagcgtcag tcagtgagtg
     1741 taatatgcgc tcttttcaag agagacttct aattttcacc ggcaattcga ccaatctcct
     1801 tattcaaatc tccgaattga acgagctccg tgagcggctc aggaaagcgg aacaatctgc
     1861 ccagaaagcg cggcggtcga agccgcggaa aagaacgcca attgagcgct ctgcacatcc
     1921 ctgatggcgg atagccgatc gcacaattga agcctgttgc cgcctgggct cgtcccttcg
     1981 atgatcctgt ctgctaccga gtgggcgcga gccggtgacc ctgcgcgatg ccgccgacta
     2041 catcatgaaa ctgccgaagg ccgagcagga tctcaaggaa tggcaaatcg ctgtggcttg
     2101 tctgatcggt gccgccgcga ctgagatagc gcacgatcag cgagcacctc tccttctgcc
     2161 ggacgaaggg caatccgccg aggcttgtcc gatcgcgtcg agttgatgat gatgcgcgcc
     2221 ctcaacggcg gagttcatca catggcgaga tcaccgagac catcgacgga cgcgaagacg
     2281 ctcaacagct cgatccgcgc aaagctcgcg gcggcttcca ccaaaggatc gagccgcgtg
     2341 caggcatcgc atcagccgaa gccgatctcg atgccgcgcc tcccttggca cgatgccgag
     2401 cagcgttgac gctggaggtc gagcgatcgc cgagccgggg ctcgggtctc gatccagccc
     2461 gttgccaaga cgagaaatct gtgggttaac gtttcccctt acgcgcgatt gcgcccggat
     2521 tgtggcatat tgatcagatc gcgaccccaa cgcgcagaga gagaggccca catggcactg
     2581 aatgacggag agattgcggt cgttaaaggg atgctgcttc gcggagatcg gcaacacgac
     2641 attgccgcct atttcggcat caacggtggg cagcattgct gagatcacca tgacaacaat
     2701 attcccatac cttgaggtcg cacgcgtgca tggtgtgctc tatgcagacg tgcttgccta
     2761 tgcagacaag ttagatcgaa gcatacgcgc atggtcgcct gattggacaa ggacagcgcc
     2821 cgcttggatc aaagacacag aggcagcgtg gtgcagagag cgggcacgac gtgacctgac
     2881 tgtgaggggc acttgctcat tgctgatcga tagcatgctg atcgatgatc acgacgactc
     2941 ggcacagcga ccagccacga tcttgtagag ccaaaatcaa tagccgcacc gccccggtat
     3001 attcagccaa gccaggccgc tctcccgcag agaaaccctc tcggcaggag gcaatcaata
     3061 aatcaagaga tcgacgctcg gcgcaccgtt aacctttgct aacggcacta gcataggatt
     3121 cccgaatcaa ccgggagctt ggccatgccg cgcaggataa tgctgtccgt tttcactatc
     3181 gcggtcaccg cggtgaccgc gggcgccgct gatctgccga cccagagccg gctcggcgcg
     3241 attttcgcgg agcctaccga ggttcgcacc cgtgccgatc ggtcccgcga acgcgtggcc
     3301 ccgatcatgc cgttcaacct gcttccaaat cccccgtgga atcgcggcgg ctactattat
     3361 ggttcgtcgt ggtcctacta cgacggcggc ccctattacg gcgggcccct ggtttttagc
     3421 ggcggtcgcc tgccctatgt gtgcggcctg tacggctatt gctgaaaggc tgcgcgaaaa
     3481 aactgatttc ttaaagcgga tcaagctgtt tctagctgtc cagtcccatt cgcaaaaata
     3541 tttcgcttca cccgtcgggc aaatcatctc tacaaattcg cgccatccca ccccaccaga
     3601 ggggcgtatc gcgatcgtca cggacgtagg ggagggatgc ggtggacgcg gcagcgttga
     3661 gcgcgcgatg ggttgcaggg cgggtcgaaa ggcctgtgag cgatcaccaa gcgagcggac
     3721 gagagatgtt gcagcggacg gcaaaatcgt gtggtcctga cgccccgacg ctggcgtcaa
     3781 gtctgcggag ttgcgtcggc ccaaccgggc ttagacaaga catatccgct gatgacggtg
     3841 gcaagagagc ccggtcaccg gggagagcgc gacataagcc gtaaaaccat tgcgtgcggg
     3901 aatgtcgggt gattccggtg tactcgttgt tactcgtgtg cgttctacca ataccaagtg
     3961 cacacgaggc cgcgggtgca gtgggcgccc ggcattcccc acgccctcaa gggggcgaaa
     4021 cgaccatgca caactcgggc gcatcgcgcc gcgagatcgc gaagccgtat ttgatgaata
     4081 cgaacgcgcc acactctcag ccgtcatacc ccgcgaaagc ggggtagcca gtattccgag
     4141 gcgtcagcga tggaaccgga aagctgcggc gtactggata ctccgctttc gcggagtatg
     4201 acgactcgtt gtggagcagc gcggcggcaa cccacgtccg tcacgtcatt gcgagccaac
     4261 ggggcgcgca ttcgcgcgac ccgttggctc acaatgacgg ccccggctgt ttgaaaattc
     4321 aatcgggaaa tggcgcaggc ggcacattca aatcatccag cgccgtagat ccccaccggc
     4381 gtgaaccggc caactcagcc cttgccgccg acttccttcg caaaggtatc gcgcaagccg
     4441 atggtccggt tgaacacgag tcgatccgcc gtcgagtccg gatcgcgcac gaaatagccc
     4501 tgccgctcga actgcatcgc gtcgttcgaa ttggtcgccg cgatcgcggg ctcgatccgt
     4561 gcgtccttga gaatttccag cgattgcgga ttgagatcgg cggcgaaatt ggcggcgtcg
     4621 gggttgggct ttgcgaacag cggattgtag atgcggattt cagccggcac cgactgcgcg
     4681 gccgaaagcc agtgcatggt cgccttgacc ttgcgtccgt cgggcgcgtt gccgccgcgg
     4741 gttgcgggat cgtaggtgca gcgcagttcg acgacctcgc cggccgcgtt cttgatcaca
     4801 tccgtgcatt tgatgaaata ggcatagcgc aaccgcactt cggtacccgg cgagagccgg
     4861 aaaaacttct tcggcggatt ttccataaaa tcgtcgcgct cgatatggag ctcgcggccg
     4921 aacgcgatgc ggcgcgtgcc ggctgtggga tcgtccggat ggttgacggc ttcgatctct
     4981 tcggtctggc cttccgggta attctcgatc acgaccttga gcggacgcag caccgccatc
     5041 cgccgcaacg cggtcttgtt gagcagttcg cgaatacaga actcgagcat gccgacgtcg
     5101 acgacgctgt tggccttggc gacgccgatc cgccggatga aatcgcgaat tgcggccggc
     5161 ggcaccccgc gccgccgcag cccggccagg gtcggcatcc gcggatcgtc ccagccggtg
     5221 acatgccgct gccgcaccag ctccgtcagc acgcgcttgg agagcaccgt gtaggtaatg
     5281 ttgaggcgtg cggattcgta ctgccggggc cgcgagggaa ccggcagatg atcgagaaac
     5341 cattcgtaga gcggccgatg gtcctcgaac tcgagcgt
//
LOCUS       DASWGM010000168         5254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1816397,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000168 DASWGM010000000
VERSION     DASWGM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816397"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VGA15_05105"
     CDS             <1..753
                     /locus_tag="VGA15_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012169221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX9587105.1"
                     /translation="VLLLDVTPLSLGIETLGGVFTRIIDRNTTIPTKKSQVFSTAEDN
                     QNAVTIRVFQGEREMAADNKVLGQFDLMGIPPSPRGMPQIEVTFDIDANGIVNVSAKD
                     KATGKEQQIRIQASGGLSEADIQKMVKDAEANAAEDKKRREAVDAKNHADALVHSTEK
                     ALAEHGSKVGESERRAIEDAVSDLKEALKGDDAEAIKAKTNTLAQASMKLGEAMYKQQ
                     AESDAAKDAAKDDVVDAEFTEVDDDKNNKKSA"
     gene            887..2020
                     /gene="dnaJ"
                     /locus_tag="VGA15_05110"
     CDS             887..2020
                     /gene="dnaJ"
                     /locus_tag="VGA15_05110"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX9587106.1"
                     /translation="MSTKRCYYETLEVDRTADESKLKAAFRKLAMKWHPDKNPGDATS
                     EVRFKEINEAYEVLKDGDKRAAYDRFGHAAFEQGAGGGGPGFGAGFASSFSDIFEDLF
                     GMAAQRGGRGGGRERGADLRYNMEITLEEAFVGKTAQIEIPVSVTCESCSGIGAKAGT
                     KPKTCSTCGGAGRVRQAQGFFTLERTCPGCQGRGQMIEDPCPSCVGTGRVTRERTLSV
                     NIPQGVEDGTRIRLAGEGEAGVRGGPPGDLYIFLSLATHLFFQRDGADLHCRVPISMV
                     TASLGGEFEVPTIDKGKTKVKVPSGTQSGRRFRIASKGMPVLRSRQTGDMYVQVVVET
                     PQNLTKKQQELLAEFEKLSSGATQPEAAGFFTKVKDFFGTRAI"
     gene            2228..2830
                     /locus_tag="VGA15_05115"
     CDS             2228..2830
                     /locus_tag="VGA15_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HEX9587107.1"
                     /translation="MPLQSSVRALKKPLRLDDEVRFLRSWIEKPLHMGAVMPSGKVLA
                     RTMAQYVDINSTGPVVELGPGTGAITSALIDHGVDQKRLVLVEYNPGFCALLRDRYPH
                     AKVVQGDAYTLRDSLWNVLSVPASAVVSGLPLVTKPMLTRLKLIRDSFAALAPGAPFV
                     QFTYSVAPPIPKSLPGVSTEASERIWMNIPPARVWVYRKR"
     gene            2879..3457
                     /locus_tag="VGA15_05120"
     CDS             2879..3457
                     /locus_tag="VGA15_05120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX9587108.1"
                     /translation="MSALKILVIPGSLRTGSLNARLAAAAAYEFAQADAEVTRISLGD
                     FPLPIYDGDLQTKSGVPKNAINLKRMIGAHHGVLIVTPEYNSSVPPLVKNTIDWVTRV
                     QDAPEARGQVFRETPFAIAAASGGRLGGTRALAALRLILSACHATVIPNQLALSFADQ
                     AYDDMDRLKHPADIEALNALVRQLIDVSQRMM"
     gene            3464..4174
                     /gene="pyrF"
                     /locus_tag="VGA15_05125"
     CDS             3464..4174
                     /gene="pyrF"
                     /locus_tag="VGA15_05125"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX9587109.1"
                     /translation="MQPSVIAPRDRLIVALDLPDVAAAEAMIARLGDSVTFYKIGYQL
                     AYAGGLPLVRQLADNGKKVFADLKLHDIGNTVARGVESVARLGATFLTVHAYPQTMKA
                     AVEAGAGSGLKILAVTVLTSYDDGDLHAAGYRLGVSDLVEARAQQAQVLGVDGLVCSP
                     EEAHSLRKIVGHQMNLVTPGIRPLGVSAGDQKRIMTPARAIAAGADYLVVGRPVMQAA
                     DPRAAAEAIQAEIAHALG"
     gene            4195..4497
                     /locus_tag="VGA15_05130"
     CDS             4195..4497
                     /locus_tag="VGA15_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX9587110.1"
                     /translation="MAKGYWIARIDVHNVEGYKDYVAQNGAVFAKYGAKFLVRGGKHV
                     PKEGSSRSRNVVLEFKDYETAVACYNSPEYVRLVAIRSPHAESDLVIIEGYDGEQP"
     gene            4761..>5254
                     /gene="dapB"
                     /locus_tag="VGA15_05135"
     CDS             4761..>5254
                     /gene="dapB"
                     /locus_tag="VGA15_05135"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX9587111.1"
                     /translation="MSDMRLIVAGAGGRMGRALTRVIAETPGAVLTGALEAPGSELLG
                     KDAGVLAGLPANGVLLSADLWSLSAEADGILDFTVPRATIANVAIAAERGLIHIIGTT
                     GLSISDDAVIQSVTSRAIVVKSGNMSLGVNLLAALVKRVAQSLDQDFDIEILEMHHRA
                     KIDAP"
BASE COUNT         1015 a         1600 c         1699 g          940 t
ORIGIN      
        1 gtgctgctgc tcgacgtgac gccgctgtcg ctcggcatcg agacgctggg tggcgtgttc
       61 acccgcatca tcgatcgcaa caccacgatc ccgaccaaga agagccaggt gttctcgacc
      121 gccgaggata accagaacgc cgtcaccatc cgcgtcttcc agggcgagcg cgaaatggcg
      181 gccgacaaca aggttttggg tcagttcgac ctgatgggca ttccgccgtc accgcgcggc
      241 atgccgcaaa tcgaggtcac cttcgatatc gacgccaatg gcatcgtcaa cgtgtcggca
      301 aaggacaagg cgaccggcaa ggaacagcag atccggattc aggcgtccgg cggtctgtcg
      361 gaggccgaca tccagaagat ggtcaaggac gccgaggcca acgccgccga ggacaagaag
      421 cgccgcgaag cggtcgatgc caagaaccat gccgacgccc tggtgcattc gaccgagaag
      481 gcgctggccg aacatggctc gaaggtcggg gagagcgaac gccgcgccat cgaggacgcc
      541 gtcagcgatt tgaaggaagc gctgaagggt gacgatgccg aagcgatcaa ggccaagacc
      601 aacaccttgg cgcaggcttc catgaaactc ggcgaggcca tgtacaagca gcaggccgag
      661 agcgatgcgg ccaaggatgc cgccaaggac gacgtggtcg acgcggagtt taccgaggtc
      721 gacgacgaca agaacaacaa gaagtctgct taaacggcaa tgatcatgac cctcattcca
      781 aagagcgcgg gagccgcgtc tcgaaggatg ggggtcattt ttcgttaagc cgtaggcggc
      841 atgctacgac gcacaaataa ggacgaactt cgggcgggtt tgacggatgt ccaccaagcg
      901 ctgctactac gaaacccttg aagtcgatcg gactgcggac gagtccaaac tcaaggcggc
      961 attccgcaag ctcgcgatga aatggcatcc ggacaagaat ccgggcgatg ccaccagcga
     1021 agtccgcttc aaggaaatca acgaagccta cgaggttctc aaagacggcg acaagcgcgc
     1081 cgcctatgac cggttcggcc atgcggcctt cgagcagggc gcgggcggcg gcggtcccgg
     1141 cttcggcgcc ggctttgcct cgtcgttctc cgatattttc gaagacctgt tcggcatggc
     1201 cgcgcaacgc ggcggacgcg gcggcggacg cgagcgcggc gccgacctgc gctacaatat
     1261 ggaaatcacg ctggaagaag cttttgtcgg caagaccgcc cagatcgaga tcccggtttc
     1321 ggtcacctgc gaatcctgtt cggggatcgg cgcgaaggcc ggtaccaagc cgaagacctg
     1381 ctcgacgtgc ggcggcgccg gacgggtgcg gcaggcgcag ggatttttca cgctggagcg
     1441 aacctgtccg ggctgccagg gccgcggcca gatgatcgaa gatccctgtc cctcatgtgt
     1501 gggcaccggg cgggtgacgc gcgagcggac cctgtcggtc aatatcccgc aaggcgtcga
     1561 agacggtacg cgtattcggc ttgccggcga aggcgaggcc ggtgttcgtg gcggaccgcc
     1621 gggcgatctc tacattttcc tgtcgctggc cacgcatttg ttctttcagc gcgatggcgc
     1681 cgatctgcat tgccgcgtgc cgatctcgat ggtgacggcg tcgctggggg gcgagttcga
     1741 ggtgccgacc atcgacaagg gcaagaccaa ggtgaaagtc ccgtcgggaa cccagtccgg
     1801 ccggcgcttt cgcattgcat caaaaggcat gccggtgctc cgctcgcgtc agaccggaga
     1861 catgtatgtc caggtcgtgg tcgaaacgcc acagaatctg accaagaagc agcaggaact
     1921 gctggccgag ttcgagaaat tgtcgtccgg tgcaacgcaa cccgaagcag cgggtttctt
     1981 cactaaggtc aaggacttct tcggtacccg cgccatttga tagcgcggtc cgcaaaggcg
     2041 caatacagct ttgcgtccga ccgcgctaca agaatagggt aagcgcgtag ttctaccgcc
     2101 aaaccacgca cactttgacg gaccatgcgc cgggccgtgc ggcttgaccc tatcggctgc
     2161 tacctatacg tctttgtgac cattttctga caccgccgcc gaattcgcgg tcccgcctgg
     2221 tagcgacatg cccttgcaat cgtccgtgcg tgcgttgaag aaacctcttc ggcttgacga
     2281 tgaggttcgc tttctgcgtt cgtggatcga gaagccgctt cacatgggcg cggtgatgcc
     2341 ctcgggaaaa gtgttggcgc gtacgatggc gcaatatgtc gatatcaatt cgacagggcc
     2401 ggtcgtcgaa cttggaccgg ggaccggcgc gattaccagc gcgctgatcg accatggtgt
     2461 cgatcagaag cgcctcgtgc tggtcgaata caatccgggc ttctgcgcgc tgctgcgcga
     2521 ccgctatccg cacgcgaaag tcgtacaggg cgacgcctat acgttacgcg actcgctctg
     2581 gaacgtgctg agcgtgccgg cgtccgctgt ggtgtccggc ctgccgctcg tcaccaagcc
     2641 gatgctgacg cgtctgaagc tgatccgcga ttctttcgca gcactggcgc cgggggcccc
     2701 gttcgtgcaa ttcacctatt cggttgcgcc gccgattccg aagtcgctgc cgggcgtgtc
     2761 cacggaagcc tccgagcgca tctggatgaa cattccaccg gcccgcgtct gggtgtatcg
     2821 caaacgctga taccgccggg cgctgccagc gcagcggacc aacgcgatcg ctaaaatcat
     2881 gtccgcgctg aaaatcctcg tgatccccgg ctcgctgcgc accggctcgc tcaatgcgag
     2941 gctcgcggcg gccgcagcct atgaattcgc gcaagccgac gcggaggtca cgcgcatctc
     3001 gctcggggat tttccgctgc cgatctatga cggcgacctg cagaccaaat ccggcgtgcc
     3061 gaagaatgcg atcaacctca aacggatgat tggcgcgcat cacggcgtcc tgatcgtgac
     3121 gccggaatat aattcctcgg tgccgccttt ggtcaagaac accatcgact gggtgacgcg
     3181 ggtgcaggat gcgcccgaag cgcgcggcca ggtgtttcgc gaaactccgt ttgcgatcgc
     3241 ggcggcgtcg ggaggcaggc tcggcggaac gcgcgcgctg gcggcgctgc ggttgatcct
     3301 gtccgcctgc cacgcgacgg ttattccgaa ccagctcgcg ctgtcgttcg cggaccaggc
     3361 ctatgatgat atggacagat tgaaacatcc ggccgatatc gaggcgctca acgcgctggt
     3421 gcggcagttg atcgacgtct cccagcgcat gatgtgaggt gacatgcaac catctgttat
     3481 cgccccccgg gaccgattga tcgtggcgct cgatctgccc gatgtggccg ccgccgaagc
     3541 gatgattgcc cggctcggcg acagcgtgac gttctacaag atcggctacc agcttgccta
     3601 tgccggcgga ttgccgctgg tccgccagct cgctgacaac ggcaagaagg tgttcgccga
     3661 tctcaagctg catgacatcg gcaatacggt ggcgcgcggc gtcgaaagcg tcgctcgact
     3721 tggcgcgaca ttcctgaccg tgcatgccta tccgcagacc atgaaagcgg cggtcgaggc
     3781 cggcgcgggc tcgggtctga aaatcctcgc cgtcacggtg ctgacctctt atgacgatgg
     3841 cgacctccac gccgccggtt atcgccttgg cgtatccgat ctggtcgagg cccgcgccca
     3901 gcaggcgcag gtgctcggcg tcgatggcct ggtgtgttca ccggaagaag ctcacagcct
     3961 gcgcaagatt gtcggtcacc agatgaattt ggtgacacca ggtattcggc ccttgggcgt
     4021 ttcggccggc gaccagaagc gtatcatgac cccggcccgg gcgattgccg ccggcgcgga
     4081 ttatctggtg gtcggcaggc ccgtgatgca agcggccgat cccagggcag cggcggaggc
     4141 catccaggcc gagatcgcgc atgcgctggg ctaagtaacg aggagaaaca caagatggcg
     4201 aagggatact ggatcgcgcg catcgatgtt cacaatgtgg aaggctacaa ggactacgtc
     4261 gcgcaaaacg gcgcggtatt tgcgaaatac ggcgccaaat ttctggtgcg cggcggcaag
     4321 cacgtgccca aggaaggctc gtcgcgctcg cgcaatgtgg tgctcgaatt caaggattat
     4381 gagaccgcgg tcgcctgcta taactcaccc gaatatgtcc gcctggtcgc gatccgcagt
     4441 ccccacgccg agagcgatct tgtgatcatc gaaggttacg acggcgagca gccgtagcgt
     4501 acgaccgaaa gagcatcgag cttctccgtc atggccgggc gtcgtcccgg ccatccacgt
     4561 cttccctgtc gttctgttgc caagacgtgg atgcccggga cataggcgag cggaagcgac
     4621 gccgtccttc ggacggctat aggcgagcgg aagcgacgcc gtccttcgaa cggccgggca
     4681 tgacgagtgg tgggggcctg attggttgcc ggaaccgccc gtcccggcta tacggcaagg
     4741 gggtcgcgag gtcttcaaaa atgtccgaca tgcgcttgat cgttgcgggg gccggcggcc
     4801 ggatgggccg cgcgctgacg cgtgtcatcg ccgaaactcc cggcgccgtt ctcaccggag
     4861 cgctggaggc gcctggctcg gagctgttgg gcaaggatgc cggcgtgctc gcgggcctgc
     4921 ctgccaacgg cgtcctgctg tcggccgatt tgtggtcgct gtcggccgag gcggacggca
     4981 ttttggactt caccgtgccc cgcgccacca tcgccaatgt cgccatcgcc gccgagcgcg
     5041 ggctgatcca catcatcggc accacaggtc tttcgatctc cgatgacgcg gtcatccaga
     5101 gcgtcacctc gcgggccatc gtggtcaaat cgggcaatat gagcctcggt gtcaacctgc
     5161 tggcggcgct ggtgaagcgg gtggcgcaat cgctggatca ggatttcgat atcgaaattc
     5221 tggagatgca tcacagggcc aagatcgatg cgcc
//
LOCUS       DASWGM010000169         4101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2372339,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000169 DASWGM010000000
VERSION     DASWGM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372339"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VGA15_05140"
     CDS             complement(<1..603)
                     /locus_tag="VGA15_05140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9587112.1"
                     /translation="MKWQRETARAFAMGSSTVAGQWIDIAAADGGTFKGYLTVPASGS
                     GPGILLLQEIFGVNSSMREVADYYAEEGYVVLAPDLFWRLEPGIELGYSEADFNKAFG
                     YYQRFDANQSIKDAADALKVLRARSECVGKVGALGFCLGGKLAYLAAARTDVDCAVCY
                     YGVGIEADLGEAAQIKGPMVFHFAELDRFAPAEAREQVKAA"
     gene            711..1301
                     /locus_tag="VGA15_05145"
     CDS             711..1301
                     /locus_tag="VGA15_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEX9587113.1"
                     /translation="MKKTTNAALKKTGGTAKPKSQPAAVERPGSWHLARTETERLLSD
                     FEFGLERLAQAYYRWKAACLAAVCDVPLTGDDVAVLNVVRMGDEPKRLSEIGQLLNRV
                     DVPNLQYATRKLVRSGLIETEGGSSRKETRYRATATGRSVTEAYAALRAATLPPMLEA
                     LDGWAAKSETTSIQLDLISSLYAQAAQVAITRRHQP"
     gene            complement(1464..2081)
                     /locus_tag="VGA15_05150"
     CDS             complement(1464..2081)
                     /locus_tag="VGA15_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9587114.1"
                     /translation="MPRLNREESQARTRSLLIEAARDEIVKKGFALASVRDIADAAGF
                     SQGAFYSNFPDKEAILLELVQRHQSEERAKIEAALSHAQGDAAKAMAGIEQWATTINS
                     DPGFSVLAIELQLQALRSPSFAQGYNDLNRKHRRALGTLVTKLFELLGRKLPADPVEI
                     ATGFIALGRGMALLSKDGEASRSGRIIVTFLKALIGSAPADGSKK"
     gene            2179..2868
                     /locus_tag="VGA15_05155"
     CDS             2179..2868
                     /locus_tag="VGA15_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587115.1"
                     /translation="MEIRSLDRVLAILATLAALTVAVMVVAILTTRGSQDFFQTARSP
                     EAYGAYLATPLVSLGLRLNLGLDNLFMILYGTFFIVLTIRLREMLDPRLLGVALAAMM
                     LTVVLDSIENHHIMTMAHSIQSGLPLSAVDGQLQMIASQVKFHSSYLAVLLFSFGFLQ
                     FGKLGRVIAIVLWCYIPCGVLISVTPVESAKALVLGRTIFFVFAFILSAALFFSQPKE
                     AGQKTSGQTTR"
     gene            2887..3249
                     /locus_tag="VGA15_05160"
     CDS             2887..3249
                     /locus_tag="VGA15_05160"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX9587116.1"
                     /translation="MPVTVITIVGRTLLALLFILAGATKIAGPQPFLDNMAAHHIPGV
                     LLPLVILLELGAGAALLLGWRLPFAAGALALFCVATAFVFHLDLADKVERTHLIKDLA
                     IAGALMIIAAGASQISMG"
     gene            complement(3316..3873)
                     /locus_tag="VGA15_05165"
     CDS             complement(3316..3873)
                     /locus_tag="VGA15_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587117.1"
                     /translation="MADLAAAPVLRPSRQAHGGRPRGVRPRATALGIADATFGQAATF
                     NTDVGFSLDVSPDKKAFTASFSGLAVNIDGKSSAPIVSRVFSFALPLSGADPGQEIPF
                     FVSGFVASEQGANGHLVFSVNDQTIVADYPGNSKNDFVQQLKYKAGDAAEVRITVFLL
                     ADRDPTSDSAVSLNVTAIDTDIVKH"
BASE COUNT          801 a         1262 c         1179 g          859 t
ORIGIN      
        1 ggcggccttg acctgctcgc gcgcttcggc cggcgcgaac ctgtcgagtt cggcgaagtg
       61 aaataccatc gggcctttga tctgcgcggc ctcgccgagg tcggcctcga tgccgacgcc
      121 gtaatagcag accgcgcaat cgacatcggt gcgcgccgct gcgagatagg cgagcttgcc
      181 gcccaggcag aagccgagcg cgccgacctt gccgacgcat tcgctacgcg cgcgcagcac
      241 cttcaacgca tcggcggcgt ccttgatcga ttgattggcg tcgaagcgct gataatagcc
      301 aaacgccttg ttgaaatcgg cttcggaata gccgagctcg atgccaggct ccagccgcca
      361 gaacagatcc ggcgcgagca caacataacc ttcctcggca taataatcgg caacctcgcg
      421 catcgaggaa ttgacgccga agatctcctg cagcagcagg atgccgggac ctgatcccga
      481 tgccggaacg gtgagatatc ccttgaacgt gccgccatcc gcggcagcga tatcgatcca
      541 ttgtcctgcc actgtactgc tccccatcgc aaacgcgcgc gcggtttcgc gctgccactt
      601 catcgtgctt tcttaattga atttttaatg taagaatcgt gattggtaaa tagcatagat
      661 tgagcaaggc ttgcctacgc gttgagcgca cgctacatca ggagaccgtt ttgaagaaga
      721 cgacaaacgc cgcgctgaag aaaaccggcg gcactgcgaa gccgaaaagc caacctgcgg
      781 ccgtcgaacg gccgggctcc tggcatctgg cgcgcaccga aaccgaacgc cttctgtctg
      841 atttcgagtt cgggctggag cggctggcgc aggcttacta tcgctggaag gcggcgtgtc
      901 ttgccgccgt ctgcgacgtg ccgctcactg gcgacgatgt ggcggtgctc aatgtcgttc
      961 ggatgggcga tgagccgaag cggctgtccg agatcggaca gctgctcaac cgcgtcgacg
     1021 tccccaatct tcaatacgcc acgcgcaagc ttgttcgctc gggcctgatc gaaaccgagg
     1081 gcggttcgtc gcgcaaggaa acgcgctacc gggccaccgc gaccgggcgt tccgtcaccg
     1141 aagcctacgc cgcgttgcgc gccgcgacgt taccgccgat gctcgaggcg ctggacggtt
     1201 gggcagcaaa atccgagacg acaagcatcc agctcgattt gatttcaagc ctctacgctc
     1261 aggcggcaca ggtcgcgatc acccgacggc atcagccctg aaagcgcgac agaggcatac
     1321 agggttcggc agatcagaaa gttcttcgtt atgagcaagg tcgtcgataa cgcgccgctg
     1381 gagtcttgat ccatagacga tccgtcgctc catgcagctg ggctacgcgc tgttgtcatt
     1441 ttccaaacaa gccaaagcgg cgtctacttc ttacttccgt cggccggcgc cgaaccgatc
     1501 aaagccttca ggaaggtgac gatgatccgg ccgctgcggc tggcctcccc gtcttttgac
     1561 agcagcgcca tgccgcggcc cagcgcgatg aacccggtgg caatttccac ggggtcggcg
     1621 ggcagtttcc ggccgagcag ttcaaacaat ttcgtaacga gcgtacccaa tgcgcggcga
     1681 tgcttgcggt taagatcgtt gtagccttgc gcgaaggagg gactgcgcaa cgcctgcaat
     1741 tgaagctcga tcgccaggac ggaaaatccg ggatcggaat taatcgtggt ggcccattgc
     1801 tcgatacccg ccatggcctt cgcggcgtcg ccctgcgcgt ggctcaacgc ggcctcgatc
     1861 ttcgctcgtt cttccgattg gtggcgttgc accagctcca gcaggatcgc ctccttgtcc
     1921 ggaaaattcg agtagaacgc gccctgcgaa aatcccgccg catcggcaat gtcgcgcacc
     1981 gaagccaagg cgaagccctt tttcacgatc tcgtcgcgtg ccgcctcgat caggagactc
     2041 cgcgtacgcg cctgactttc ctcgcgattt aaccttggca ccggatccaa ctcccgaatt
     2101 acaaataaca gttgattttt ggatatcaat tgatatttat attattataa gccgatttgg
     2161 cagcgctgga ggtatatcat ggagatcagg tcattggatc gcgtgctcgc aattctggcg
     2221 acgctggccg cgctgacggt tgccgtcatg gtcgtcgcca tcctcaccac gcgcggttct
     2281 caggatttct tccagaccgc acgctcccct gaagcctatg gcgcgtatct ggcaacaccc
     2341 ctcgtctccc tcggcctgcg gctcaacctc ggcctcgaca atctcttcat gatcctttac
     2401 gggacattct tcatcgtgct gaccatccgg ctgcgggaaa tgctggaccc gcgcctgctc
     2461 ggcgtcgcgc tcgctgcgat gatgctgaca gtcgttctgg actcgatcga gaaccatcac
     2521 atcatgacga tggcacactc gatccagagc gggctgccgc tttccgcggt tgacggccag
     2581 cttcagatga tcgcctccca ggtcaagttt cacagcagct atctggcggt tctgctgttc
     2641 tcgttcggct tcctgcagtt cgggaaactg ggccgcgtca tcgcgatcgt gctgtggtgc
     2701 tacattccgt gcggcgtgtt gatctcggtg acgccggttg aatcggccaa ggcactcgta
     2761 ctcggacgaa cgatcttctt cgtctttgcc ttcattctct cagccgccct gttcttttcg
     2821 caacccaagg aggccggcca aaagacatcc ggtcaaacca cccgataacg gaaaaaggag
     2881 cacaccatgc cggttactgt cattacgatt gttggtcgca cccttctcgc attgctgttc
     2941 attctcgccg gagcgacgaa gatcgccggc ccgcaacctt tcctggataa tatggccgct
     3001 caccatattc caggcgtgtt gctaccgctg gtcattctct tggaactggg cgccggcgcg
     3061 gccctgctgt tgggctggcg gctcccgttc gctgcggggg cgctggcctt gttttgcgtc
     3121 gctaccgcct ttgtatttca tctcgacctc gccgataagg tcgagcgaac gcatttgatc
     3181 aaggacctcg caattgccgg ggcgctgatg attatcgcgg ccggcgcgtc gcaaatctca
     3241 atgggatagt cgtggcgcgc ctgaccggtg gcgatgcccg cactaggccg cgcgcgggcg
     3301 caacccctcc gcacgtcagt gtttgacgat atccgtatcg atggcagtaa cattcaagct
     3361 tacggccgag tccgatgtcg gatcacggtc ggcaagaaga aacacggtga ttcgaacctc
     3421 agccgcatcc ccagccttgt atttgagctg ctggacgaag tcgttttttg aatttcctgg
     3481 ataatcggca acgatggttt gatcgttgac gctgaatacc agatggccgt tcgccccttg
     3541 ctcggaagca acaaagcccg acacgaagaa gggaatctcc tgaccgggat cggcgccgga
     3601 cagcggaaga gcaaatgaaa atacgcgcga gacaatcggc gcggacgact tgccatcgat
     3661 gttgacggcc agaccactga agctggccgt aaaggctttc ttgtccggac tgacgtcgag
     3721 gctgaagccc acgtcagtgt tgaacgttgc cgcctggccg aatgttgcgt cagcaatacc
     3781 gagcgcggtg gcacgcggtc gaacaccgcg gggccgcccc ccatgtgctt gccgagacgg
     3841 tcgaagcacc ggcgcggcag cgagatcggc cacccggatc ggtttttctc tgcgactcca
     3901 atcttttgaa cggggcagcc attccccaaa gcgggttgtg caacgcagca tggccggtac
     3961 aacaggtttt ctccaagagg tgctatgtga ctgacatcaa tgccgacgcc tgggtttcct
     4021 ccaaccaccg cccgatgggt tttgccgaat tcgtgatcgt catcgcgtcg atcatggccc
     4081 tgaatccgct ggcgatggac a
//
LOCUS       DASWGM010000170         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_428968,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000170 DASWGM010000000
VERSION     DASWGM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428968"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VGA15_05170"
     CDS             complement(<1..587)
                     /locus_tag="VGA15_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX9587118.1"
                     /translation="MVVSRRGASARAPRTTFESVSDTRIVVGDCVAEMSKLPAGSVHL
                     VFADPPYNLQLKGDLKRPDESHVDAVNNDWDKFSSFAAYDDFTRAWLLACRRIMKPSA
                     TLWVIGSYHNIFRVGSIMQDLGFWVLNDIVWRKTNPMPNFRGRRFTNAHETMIWAARD
                     EKAKGYTFNYEALKAANEDVQARSDWLIPLCTGDER"
     gene            825..1754
                     /locus_tag="VGA15_05175"
     CDS             825..1754
                     /locus_tag="VGA15_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX9587119.1"
                     /translation="MRYDDFRRSDDIDDRRGDDGGGGGGGFGLPMGGGGLGIGTIIVL
                     GLVGYAFGIDPRILIGGAQILTGGGQAPSYQSDRRSAPAKTGAPTDEMGNMISGILGE
                     IDDRWSEIFQASGQSYSGPRIVLFRNATNGGRCGMAQAAMGPFYCPPDKQIFLDTGFF
                     RQVETRFRGCSGNACKFTAAYIIAHEAGHHIQNLLGILPRVTRLQQQAGSKAESNALQ
                     VRVELQADCLSGVWVNREEKKRPGFLEAGDIDAALTTASAIGDDTLQRQATGRVVPDS
                     FTHGSAAQRKQWFMTGYQQGAVKACDTFSAGNL"
     gene            1904..>2082
                     /locus_tag="VGA15_05180"
     CDS             1904..>2082
                     /locus_tag="VGA15_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX9587120.1"
                     /translation="MSSIDESKKFVPLDIAVLTISDTRSLADDKSGTTLSERLTAAGH
                     RLAAREIVVDDVEAIR"
BASE COUNT          396 a          621 c          688 g          377 t
ORIGIN      
        1 cgttcgtcgc cggtgcagag tgggatcagc cagtcggaac gcgcctgcac gtcctcgttg
       61 gcggccttca gggcctcgta gttgaaggtg tagcccttgg ccttctcgtc gcgcgcggcc
      121 cagatcatgg tttcgtgggc attggtgaag cggcggccgc ggaaattcgg catcggattg
      181 gtcttgcgcc agacgatatc gttgagaacc cagaagccca agtcctgcat gatcgagccg
      241 acgcggaaaa tattgtggta ggagccgatt acccacagcg tcgccgacgg tttcatgatg
      301 cggcggcagg ccaagagcca ggcgcgggtg aaatcatcat aggcggcgaa ggacgagaac
      361 ttgtcccagt cgttgttgac ggcgtcgaca tgggattcgt cggggcgctt gagatcgccc
      421 ttgagctgca aattgtacgg cggatcggcg aacaccaggt ggacggagcc tgccggaagc
      481 ttcgacatct cggcgacgca atcgccgacg acgatacggg tatccgaaac ggactcgaat
      541 gtggtgcggg gcgcccttgc agacgccccg cgacgcgaca caaccatgac tcaaatgctc
      601 tgactcaggc gacgctgccg gcgacgcggg actaaacaac cgactggcca tacattgacg
      661 tgccaaggta aacatcgggt tatcgtgccg cgcctggtag gcattgattg ctaggcaatt
      721 tttgccggcc gggctattga atcacgcaat ggaaatttta cgtgactggc gcgttgtgta
      781 tcggcgagtt cgctgccagc cagccggaat gaggaaaaat caccatgcgc tacgatgact
      841 tccgccgcag cgacgacatc gatgatcgtc gcggcgatga tggcggcggc ggtggcggcg
      901 gcttcggtct tccgatgggc ggcggcggcc tcggcatcgg caccatcatc gtgctcggtc
      961 tcgtcggtta cgccttcggc atcgatccgc gcatcctgat cggcggcgcg cagattctta
     1021 ccggtggcgg acaggcgccg agctatcaat ccgatcgcag gtccgcgccc gccaagaccg
     1081 gtgcgccgac cgacgagatg ggcaacatga tctccggcat cctcggcgag atcgacgatc
     1141 gctggagcga gatcttccag gccagcggtc aatcctattc gggtccgcgc atcgtgctgt
     1201 ttcgcaacgc caccaatggc gggcgctgcg gcatggcgca ggcggcgatg gggccgttct
     1261 actgcccgcc ggacaagcag atttttctcg ataccggatt cttccgtcag gtcgagacgc
     1321 ggtttcgcgg ctgctcgggc aatgcctgca aattcaccgc ggcctatatc atcgcccatg
     1381 aagccgggca tcatatccag aacctgctcg gcatcctgcc gcgcgtgacg cggttgcagc
     1441 agcaggccgg cagcaaggcg gagtccaacg cgctgcaggt gagggtcgag ttgcaggcgg
     1501 attgtctgtc gggcgtctgg gtcaaccgcg aagagaagaa gcgcccgggc ttcctcgaag
     1561 ccggcgacat cgatgcagcg ctgaccacgg catcggcgat cggcgacgat acgctgcagc
     1621 ggcaagccac cgggcgggtg gtgccggact cattcaccca cggctccgcc gcgcagcgaa
     1681 agcaatggtt catgaccggc tatcagcagg gcgcggtgaa ggcctgcgat accttcagcg
     1741 cgggcaactt gtaaacgagt gttcgtgccc cggacgcggc gcagcgcgtt agcggtgcgc
     1801 tgctgatccg gggcccatga aacgtcaact gcgagtgggt cccggcgctg cggagcagcg
     1861 tgaaggacgc tgcaccgcgt ccgggacacg aggataaagc gcaatgtctt ccatcgatga
     1921 atcaaaaaag ttcgtgccgc tcgatatcgc ggtgttgacg atttccgaca cgcgctcgct
     1981 tgccgatgat aaatccggca ccacgttgtc ggagcggctc accgcggccg gtcaccggct
     2041 tgcggcacgc gaaatcgtcg tcgacgatgt cgaggcgatc cg
//
LOCUS       DASWGM010000171         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1397362,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000171 DASWGM010000000
VERSION     DASWGM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397362"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VGA15_05185"
     CDS             complement(<1..1178)
                     /locus_tag="VGA15_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9587121.1"
                     /translation="MTPVEIEKIFRDETGRALATLIRLVGDFDLAEDALQDAFATALE
                     RWPVADAPSNPRAWLVNVGRNKAIDRVRRSIAFRSKQTELAHELLFNATASDEAADMM
                     AFDDDMLRLIFICCHPAFAAEVQVALTLRTVCGLTTAQVARAFLASEDAMAQRLVRAK
                     QKIRLAGISYEVPDREALEPRLRAVLAVIYLVFTEGYVATSGEDLMRPDLAREAIRLG
                     RLLDALMPQRPEIKGLLALMLLHDARRAGRETAGGDIVLLEEQDRALWDHGQIAEGLR
                     LVEEALRMPGRPQSYAVQAAIAALHAQARNYDDTDWPQIAGLYEVLLRISPSPVIELN
                     HAAVVSMVDGPGRALDLIDALEARGSLKGYELMPAVRADLLRRLGRRDAARDAYRAAT
                     S"
     gene            complement(1287..1637)
                     /locus_tag="VGA15_05190"
     CDS             complement(1287..1637)
                     /locus_tag="VGA15_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9587122.1"
                     /translation="MQYLLMIYQNEAESGKMDAAAGKKMTEEYGVFTQSIIQSGNFKA
                     GDALQPTTTATTVRVRDGKTLTTDGPFAETREQLAGYYLVEAKDLDAALAMAARIPGA
                     KTGSIEVRPIRVYN"
     gene            1787..>1959
                     /gene="rnpB"
                     /locus_tag="VGA15_05195"
     ncRNA           1787..>1959
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGA15_05195"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          357 a          644 c          605 g          353 t
ORIGIN      
        1 gatgtcgccg cgcgataggc gtcgcgcgcc gcatcacgcc ggccgagccg gcgcaacaga
       61 tcggcgcgga ccgccggcat caactcgtaa cccttgagac taccgcgggc ttccagcgcg
      121 tcgatcagat cgagcgcgcg ccccggcccg tcgaccatcg agacgacggc ggcgtgattg
      181 agctcgatca ccggcgacgg gctgatccgc aacagcacct catagaggcc ggcgatttgc
      241 ggccagtcgg tatcgtcata attccgcgcc tgcgcatgca atgcggcgat cgcggcttgc
      301 acggcgtagg attgcggccg gcccggcatc cgcaaggcct cctccaccaa cctcagtcct
      361 tcggcgatct ggccatggtc ccacagcgct cgatcctgct cttccagcag cacgatgtcg
      421 ccgcccgcgg tttcgcgccc ggcgcggcgc gcgtcgtgca gcagcatcaa ggccagcaac
      481 cccttgatct cgggccgttg cggcatcagc gcgtcgagca ggcggcctag ccggatcgct
      541 tcccgcgcca gatccggacg catcaggtcc tcgcccgaag ttgccacata accttcggtg
      601 aagacgagat agatcaccgc gagcaccgcg cgcaggcgcg gctccagggc ttcgcgatcc
      661 ggcacctcgt aggaaattcc ggcaaggcgg atcttctgtt tggcgcggac gaggcgctgc
      721 gccatcgcgt cctcgcttgc cagaaaggcg cgcgcgacct gcgcagtggt cagcccgcag
      781 accgtgcgca acgtcagcgc gacctgaact tcggccgcga atgccggatg gcaacaaatg
      841 aagatcagcc gcagcatgtc atcgtcgaac gccatcatat cggcggcttc gtcggaggct
      901 gtggcattga acagcaactc gtgcgccagt tcggtctgct tgctacgaaa cgcgatgctc
      961 cggcggaccc gatcgatcgc cttgttgcgt ccgacgttga ccagccaagc gcgcggattg
     1021 gatggcgcat cggcgacggg ccagcgttcc agcgctgtgg cgaaggcatc ctgcaatgcg
     1081 tcttcggcga gatcgaaatc gccgaccagg cggatcagcg ttgccagcgc ccgacccgtc
     1141 tcgtcacgga atattttttc gatctcgacc ggagtcatgc gattccatct ttcacctatt
     1201 cgtcattccg gcgcgtccca acgggtccgc gcaaagcgcg gcccgatgac aggctcggca
     1261 cgaagccgga atgacggcag tggctgtcag ttatagaccc tgatcggcct gacctcgatg
     1321 gaacccgtct tggcgccggg aatgcgcgcg gccatggcga gcgcggcatc gagatccttg
     1381 gcttcgacca gatagtaacc ggcgagctgc tcgcgcgtct cagcgaacgg accatccgtc
     1441 gtcaacgtct tgccgtcgcg aacgcgcacg gtggtcgcgg tcgtcgtcgg ctgtagtgcg
     1501 tcgccggcct tgaaattgcc gctctggata atggactggg tgaacacacc gtattcctcg
     1561 gtcattttct tgcccgctgc ggcgtccatt tttccggact cggcttcgtt ttgatagatc
     1621 atcaacaaat actgcatctc gttaactcct gttgagcggc gccattcgcc gacatccagt
     1681 cgaacgggcc aaccgcaaaa cgacatcttc aggataattt attttgacag gcatcgcggg
     1741 ttttatcggc ttgacgcggc gacattgagc ctccatgcct ttggcgtcag tcggccggac
     1801 ggccgctcct gctacggccg aaaggctgcg ggagaggaaa gtccgggctc catcgacata
     1861 cggtgccgga taacgtccgg cgggggcaac cccagggaca gtgccacaga gaacgaaccg
     1921 cttcccccac ccctaaaggg agggggcgag caagggtga
//
LOCUS       DASWGM010000172        11489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1397492,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000172 DASWGM010000000
VERSION     DASWGM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397492"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VGA15_05200"
     CDS             complement(<1..181)
                     /locus_tag="VGA15_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587123.1"
                     /translation="MRATVDTRLIGRLGTRTERSEQSKACGKRDAFHAFSPNGCASFF
                     LTLRADNLPETGKRQY"
     gene            340..822
                     /locus_tag="VGA15_05205"
     CDS             340..822
                     /locus_tag="VGA15_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX9587124.1"
                     /translation="MFAASALIPNQHCTATFAGAAVVLLHLGAAITPARSAGGEAVKI
                     SDAWVHAAEQVGGNIALSMTIKNEADVADMLLRVRCPVANFSAKHTVDRGEGSPAMRE
                     IPSIPIAASSTTVLKSDAYHVMLLQTRQALAAGEAFNCSIVFQKAGTIETEVKVQGLP
                     "
     gene            934..1269
                     /locus_tag="VGA15_05210"
     CDS             934..1269
                     /locus_tag="VGA15_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587125.1"
                     /translation="MKRFKNHRPLMRTSVLATVLAAVGFAGPLPARAADEVTHDRLLN
                     ADKEPGNWLLHHKNFSATRFSSLKDINRDNVKNLKVAWTMHLGGVEGGGIWTHGGLEG
                     TPIVENGFM"
     gene            1291..2721
                     /locus_tag="VGA15_05215"
     CDS             1291..2721
                     /locus_tag="VGA15_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9587126.1"
                     /translation="MYKIDAHGGQGTLLWKMDPKTDHDWAGAVACCGVDNRGVALWGN
                     LVISHTLDGRLIATNKDNGQVAWQRQVANPDKGEVITGAPLIVKNMAITGVAGAEYGI
                     RGWVAATDLATQKEVWRTFTIPGKGEPGHDTWKDDKNAAASGGGSTWVTGSYDPATDT
                     IIWGVGNPGPDWDNQYRPGDNLYTDSSLALDANTGKIKWHYQHTPNDPYDYDSVAENV
                     LVDVPGPNGTTLKLALEADRNGFAYAIDRTTGKFVWGLPFVKKVTWTKGLDQESGKPI
                     EYDPNKQVQRYNASVTPHRDNKIADICPGNMGGKNWPPTAYNPDLKLWYIPVIESCNR
                     ITVEESTPEKLKARDFWTGGGPSQPFKITGSVTAIDVTTGKVAGKLETPYPNLGGILA
                     TPDLVFSGQPSGEVMALDAKTLEKRWEFNTGGGVNAPPMTFSVDGKQYIAILVGLGGA
                     WDKWFIDATPELKRMQPGSMLYVFAL"
     gene            2789..3352
                     /locus_tag="VGA15_05220"
     CDS             2789..3352
                     /locus_tag="VGA15_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9587127.1"
                     /translation="MTSGKMSSIAALIAAAAVAAALSAPCTALGQSATTSDPTDAGKA
                     VFKRANCFGCHKWHGNGGGGYGGDALSLRKTELTREQIIETVGCGRPGTGMPFFIRGA
                     YDTIKCYDMNRQDAGSQMPPEANTFLRPNDIEAVADYVIARIKGKSEPNYAECVSFFG
                     DTSRVCDIYKTQAEPTANSAAGAGKAQ"
     gene            3349..3999
                     /locus_tag="VGA15_05225"
     CDS             3349..3999
                     /locus_tag="VGA15_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587128.1"
                     /translation="MIHRAQRLIATSVFILSLLVTAAATTPAAAQDPDGNDLRDIQLG
                     KPATDLPDAGYVNLVCADDSRKTLTTWLGWRDCPADASGFHAIKFGYDSATSPDGTIV
                     AGHPAVLTLLIDDAGTASGLRIETDPKARLYIRKKAFLFGIQVKSRYGLDGWTCTQAQ
                     PNAGEQPVGGVYVSERCRKNTQNRSIVVERNLFRKPDQDMKNFVDETRMTILRTKD"
     gene            complement(4294..5493)
                     /locus_tag="VGA15_05230"
     CDS             complement(4294..5493)
                     /locus_tag="VGA15_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587129.1"
                     /translation="MSKSFKALGLAVGALALTHLPASAQTKVTNEGISATEIVIGTHQ
                     DLSGPIKVWGVPVSNGMKMAVEEINAAGGINGRKIRMILEDSGYDPKRAVLASQKMIE
                     RDKIFAMVGPMGSPTVLAAQDILFDAGVLQLFPLTAAEFTFKFDPAKPQERLKFNNLL
                     PYVESTRAALKYMIEAKNFKKPCIMHQDDEYGKNVLDGFTQQLEAMKLTPASITSYKR
                     GASDFSAQVAKMKSDGCDLVLLGTVIRETIGAMSEAKKLGWDVTFLGATPTNVLEVPT
                     LGKEAVEGLYAAALFEIPYEDTAKGKVKDWLVNYKKMFGSDANTQAIIGYNSVMTFAH
                     YVNKAGKDLTGQKLLDALESGDKFLDIFNSPPTKFSKTDHLASAVTQIQQIKNGRWVL
                     MKDSLSF"
     gene            complement(5575..7413)
                     /locus_tag="VGA15_05235"
     CDS             complement(5575..7413)
                     /locus_tag="VGA15_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9587130.1"
                     /translation="MARPAVLTVADTIANSFLLAAETRGDRTAIREKKFGVWQPTSWR
                     QWLQISKEIAYGLHAIGFRPGDVASIVANAVPEWVFADMGILCAGGVSSGIYPTDSSS
                     QVEYLVNDSSTRVIFAEDEEQLDKILSCRARCPTLERIVVFDMEGLSGFSDSMVMSLA
                     EFTALGRNHIQGREALWEEMVASRGANDLAILVYTSGTTGPPKGAMHSNRGVTHQMRH
                     ANDLFPSTDSEERLVFLPLCHVAERIGGYYISLALGSVMNFAESPETVPDNLREVQPT
                     AFLAVPRIWEKFYSGITIALKDATPFQNWMYRHALAVGYRMTDCRLDGETPSLSLRLV
                     NRAAYWLVFRNIRCMLGLDRCRLAFTGAAPIAPDLIRWYLALGIDIREVYGQTENCGV
                     ATIMPPDRIKLGSVGKAAPWGEVGISPEGEILIKGDFLFMGYLNQPERTAETIDAKGW
                     LHTGDVGSIDNEGFVKITDRMKDIIITSGGKNITPSQIENQLKFSPYISDTVVIGDKR
                     PYLTCLVMIDQENVEKFAQDHDIPFTNYASLCRAAEIQDLIQREIEAVNANFARVETI
                     KKFFLIERQLTPEDEELTPTMKLKRNFVNKRYAIEINAMYGDRAVA"
     gene            complement(7549..8328)
                     /locus_tag="VGA15_05240"
     CDS             complement(7549..8328)
                     /locus_tag="VGA15_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587131.1"
                     /translation="MSAPDIILRLSNIESYYGPIMAIRGISIEVPRGKIITLLGANGA
                     GKTTVLKTISGILDPQKGSIEFLGKPIQRMEADKIVRLGLSHVPEGREVFPFLSVREN
                     LMMGAYPRKDRDDVAEDLERVYGYFPRLKERLNQPAGQLSGGEQQMLAIGRALMNRPT
                     LLLLDEPSLGLSPILVKEIFTIIRRVNEEQGMSILLVEQNAKVALETAHYGYVLEIGR
                     VVMNDSCERLLHSKDIQEFYLGAKEAGARGERRWKKKKTWR"
     gene            complement(8340..9098)
                     /locus_tag="VGA15_05245"
     CDS             complement(8340..9098)
                     /locus_tag="VGA15_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587132.1"
                     /translation="MSYFRAENLSLHFGGLKAVDAVSFAVEKGEILSIIGPNGAGKSS
                     IFNLISRIYSPTSGRIYFEDQDITQEPAFGIAKLGIARTFQNIELFENATVLSNLLVG
                     RHRHSTTQLWQELLFLPSVRSNEKIHRRRVEQVIEFLDLEPYRDKLISGLPYGVRKVI
                     ELARALCTEPKLILLDEPSSGLNVEETDDMSFWIRDMRTELGITVLMVEHDMTLVNRV
                     SDRVIAVNYGKVLAIGSPAEVQRHPDVVAAYLGT"
     gene            complement(9095..10246)
                     /locus_tag="VGA15_05250"
     CDS             complement(9095..10246)
                     /locus_tag="VGA15_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587133.1"
                     /translation="MRFMFKTDYEDDIRLFSHSGYVVSYGILLAVLVIAPFVLSSYLV
                     SQLVFVCIYATVGVGLMILTGFTGQASLGHAAFLAIGAYTAAYLQQFNVPFPVYFLAA
                     GLLTGIIGALVGFPALRLQGIYLVIATISFAFIVEEILARWESVTHGNEGMRVKTIQL
                     LGTSVPRDSPAFYFLCLGVLILTIVGTLNLLRSPTGRAFVAIRDSETAARSMGVNVSL
                     YKVKSFAISAGITGFAGCLFAHKLSFISPEMFTLQLSIEFIIVILIGGAFSPHGAVLG
                     AIFIVMIDPFLTYLKDDVPGMVAGVAATLGAGKESVAHIQSNVAAFASANGLKGAIYG
                     IIIVLFVLFEPLGLYGRWLKIKLFFQLFPLYKRATFKRQKIYVKSERNR"
     gene            complement(10256..11131)
                     /locus_tag="VGA15_05255"
     CDS             complement(10256..11131)
                     /locus_tag="VGA15_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587134.1"
                     /translation="MLDFVQQLVSGIALGCVYGLIALGFVLVYKATEVVNFAQGDLMM
                     LGGFFAFTFIGILGLNYWIGFAGAVAAMALFGMLAERLVVRPILGYPQFSIIMATIGL
                     GYFLRSVVGMIWGTDDFKIETPFSQGVLRIGSLVLAYDKLSVIAATIILCALLYLFFN
                     KTTLGTAMRASSENMLAAYYMGIPVKRVVSIVWAISAAVATCAGVLLAPITFIHSNVG
                     LVLGLKAFPAAVLGGFGSIPGAVVGGVLIGVIESMAGFYLPQGWKDVAPYIVLLVVLL
                     LKPEGLFGLRMRKKV"
BASE COUNT         2398 a         3450 c         3454 g         2187 t
ORIGIN      
        1 cgtattgacg ctttccggtt tcaggaagat tgtcggcacg cagcgtcaaa aaaaatgacg
       61 cgcatccatt gggagagaac gcatgaaatg catcgcgctt gccgcaagcc ttgctttgct
      121 cgctccgttc ggtgcgagtg cccagacggc cgatcaggcg ggtgtccact gtggcgcgca
      181 ttacgaactc gggtcggaca tcgcgcgagg tgcgaaaaat accagcagcg gtcgtgcggg
      241 ttcgatcgtc aagaatcttg gcggctgaac cgtgcgacga tcttcatccg agcattcgca
      301 tcgagatccc ggaccatcga tcagagggga aaacgatccg tgttcgcggc ttcggcattg
      361 attccgaatc aacattgcac cgcaactttt gcaggcgctg ccgttgtatt gcttcatctg
      421 ggagctgcga tcactcctgc ccgtagcgct ggaggcgaag ctgtcaaaat ttccgatgcg
      481 tgggtgcatg cagccgagca ggtgggcggc aatatcgcgt tatcgatgac gataaagaac
      541 gaagccgatg tcgcggacat gctgctgcgc gtgcgatgcc cggtcgcgaa tttctccgcg
      601 aagcatacgg tcgatcgcgg cgaagggtct cctgccatgc gcgagatccc ctcgataccg
      661 atcgcggcga gcagcacaac cgttctcaaa tccgacgctt atcacgtcat gctgctgcag
      721 acacggcagg cgctcgccgc gggcgaggca ttcaactgct ccatcgtttt tcagaaggcc
      781 gggacgatag agacggaggt gaaagtccag ggattaccat gagcgtgcga cagcaagcga
      841 tttgatttgc tgaaacaaga cttctcaacc gtgcgcccgg acgtgccggg cgtgcaatgc
      901 aaaacgaagt catcaacgag gaggtaaacg accatgaaac gtttcaagaa tcatcgaccc
      961 ctcatgcgga catccgtgct cgcaactgtg cttgcggctg tcggctttgc cggccccctg
     1021 cccgcccgcg cagcggatga ggtcactcac gaccggctgc tcaacgcgga caaggagccg
     1081 ggcaactggc tccttcatca caagaacttc tcggcgaccc ggttttctag tctcaaggat
     1141 atcaacaggg acaatgtgaa gaacctcaag gtcgcatgga cgatgcatct tggcggcgtc
     1201 gagggcggcg gcatctggac ccatggcggc ctggaaggca cgccgatcgt cgagaacggt
     1261 ttcatgtagg tcaccgacgg atggggttcg gtgtacaaga tcgacgccca tggcggacaa
     1321 ggcaccctgc tctggaagat ggatcccaag accgatcatg actgggccgg cgccgtcgcg
     1381 tgctgcggcg tcgacaaccg cggcgtcgcg ttgtggggca acctggtcat ctcgcacacg
     1441 ctcgatggca ggctgatcgc gaccaacaag gacaacggcc aggtcgcctg gcaacgccag
     1501 gtcgccaatc ccgacaaggg cgaagtcatt accggcgcac cgctcatcgt caagaacatg
     1561 gcgataacag gcgtggccgg cgcggagtac ggaatccgtg gctgggtcgc ggcgaccgat
     1621 ctggcgaccc agaaagaagt ttggcgcacc ttcacgattc caggcaaggg cgagccgggt
     1681 cacgacacct ggaaggacga caagaacgcc gcggccagcg gcggcggttc gacctgggtc
     1741 acgggaagct acgatccagc gaccgacacc atcatctggg gcgtcggcaa tccaggcccg
     1801 gactgggaca accagtatcg gcccggcgac aatctttata ccgacagttc gctcgcgctc
     1861 gatgccaaca ccggaaagat caaatggcac tatcagcaca cgccgaatga tccctacgac
     1921 tacgacagcg tcgccgagaa cgtcctggtc gatgtgcccg gacccaacgg cacaacgttg
     1981 aagctcgcgc tggaagcgga tcggaacggc ttcgcctacg cgatcgaccg caccaccggc
     2041 aagttcgtgt ggggactgcc cttcgtgaag aaagtgacct ggacgaaagg cctcgatcag
     2101 gaatccggca agccgatcga gtatgatccg aataaacagg tgcagcgtta caacgcatcc
     2161 gtgacaccgc atcgcgacaa caagatcgcc gatatctgtc ccggcaatat gggagggaag
     2221 aattggccgc ccacagccta taatcccgat ctcaagcttt ggtacattcc cgtgatcgaa
     2281 agctgcaacc ggatcaccgt cgaggagtcg actccggaga agctgaaggc gcgtgatttc
     2341 tggaccggcg gcgggccgag ccagccgttc aagatcaccg gcagcgtgac cgcgatcgac
     2401 gtaaccaccg gcaaagtcgc gggcaagttg gaaacgcctt acccgaatct gggcggcatt
     2461 ctcgcgacac ccgatctggt attctctggc caaccctccg gcgaagtgat ggcgcttgac
     2521 gccaaaacac tggagaagcg ctgggagttc aacacgggcg gcggtgtcaa tgcgccgccg
     2581 atgaccttct cggtcgacgg caagcagtac atcgcgatcc tggtcggctt gggcggcgcc
     2641 tgggacaagt ggttcatcga tgccaccccg gaactgaagc ggatgcagcc cggctcgatg
     2701 ctctacgtgt ttgcactcta acctcacatc gtatggacgg atcgggccac ggcccgatcc
     2761 gtctgccttt ctcaggcaag aaaagccaat gacatcaggc aaaatgtctt cgatcgcggc
     2821 gttgattgct gcagccgccg tcgcggcggc cctgtcggcg ccgtgtacgg ctctcggcca
     2881 atcggcaacg acctcggatc caaccgatgc cggcaaggcg gtgttcaagc gggccaactg
     2941 ctttggctgc cataagtggc acgggaacgg cggcggcggt tacggcggcg atgcattgtc
     3001 gttgcgcaag accgaactga cgcgcgagca gatcattgaa acggtcggct gcggaaggcc
     3061 aggcacgggc atgccgttct tcatccgcgg tgcttacgac acgatcaagt gctacgacat
     3121 gaaccgccag gatgctggca gccagatgcc gcccgaggcc aatactttcc ttcgtccgaa
     3181 cgatatcgag gccgtcgcgg actatgtgat cgcgcgcatc aagggcaaga gcgagccgaa
     3241 ttacgccgaa tgcgtcagct ttttcggtga tacttcgcgc gtctgcgata tctacaagac
     3301 gcaggccgag ccgaccgcaa attccgccgc gggcgcagga aaagcgcaat gatacatcga
     3361 gcgcagcgat tgatcgcgac gtccgttttc attttgtcgc tgctggtcac ggcggcggcg
     3421 acgacgccgg ctgcggccca ggaccccgac ggcaacgacc tgcgcgacat ccagctcggg
     3481 aagccggcga cggacttgcc tgacgcgggc tacgtcaacc tcgtctgcgc ggacgattcg
     3541 cgaaaaacgc tgacaacctg gttgggctgg cgcgactgtc ccgctgatgc ctcgggattt
     3601 cacgcgatca aattcggtta tgactctgcc acaagccccg atggtacgat cgtggcgggc
     3661 cacccggcgg ttctcacgct gctgatcgac gatgccggaa ccgccagcgg gcttcgaatc
     3721 gagaccgatc cgaaagcgcg gctgtatatc cgcaagaagg cctttctgtt cggcatccag
     3781 gtgaaatcgc gatacgggct cgatggttgg acctgcacgc aagcccagcc caacgccggc
     3841 gagcagcccg tcggcggcgt ttacgtcagc gaacgctgta ggaagaacac ccaaaaccgc
     3901 tctatcgtcg tcgaacgcaa tctgtttcgt aaaccagatc aggacatgaa gaacttcgtc
     3961 gacgaaacgc gaatgacgat cctgcgaaca aaagattaga tcgcgatcca acgacgccga
     4021 attgcagcac gatcaaaacg ccggaagacg acagcaaacg tttgggcgca tctcgaagcc
     4081 tgccatccgg agctggacat cggccggccg ctcgacatcc tcatggtgat gatatccttc
     4141 ggatcggcgg tgacgaattt acgggcgatc gcgggggagc cggctcagcg acggcccaag
     4201 cattggccga tgaaaataga gatcgaagaa atcaggaggg ctgtgtgcga gactcgcgcg
     4261 cagccctcca tcgttcaaaa cgagagatcg tcgtcagaac gagagactgt ccttcatcag
     4321 cacccagcgg ccgttcttga tctgctggat ctgcgtcacg gcgctggcga gatggtcggt
     4381 cttggagaat ttggtcggcg gggaattgaa gatatccagg aacttgtcgc cggactccag
     4441 tgcgtcgagc aacttctgac ccgtcagatc cttgccggcc ttgttcacgt agtgcgcgaa
     4501 cgtcatcacc gaattgtagc cgatgattgc ctgggtgttg gcgtccgagc cgaacatctt
     4561 cttgtagttg accagccagt ccttcacttt tcccttggcg gtgtcctcgt acggaatctc
     4621 gaagagagcg gcggcgtaaa gcccttccac ggcttccttg ccgagtgtcg gcacttcaag
     4681 cacgttggtc ggcgtggcgc ccaggaaggt cacatcccag cccagcttct tggcttcgct
     4741 catcgcgccg atggtttcgc ggatcaccgt gccgagcagg accagatcgc agccgtcgga
     4801 tttcatcttg gcgacctgcg cgctgaagtc ggatgcgccg cgcttgtagc tggtgatgga
     4861 ggctggcgtc agcttcatgg cctcgagctg ctgcgtgaag ccatcgagca cgtttttccc
     4921 gtactcatcg tcctgatgca tgatgcaggg cttcttgaaa ttcttcgcct cgatcatgta
     4981 tttgagcgca gcgcgggtgc tctcgacata gggcagaagg ttattgaact tcagcctttc
     5041 ctgcggcttg gccggatcga acttgaaggt gaattcggcc gcggtcagcg gaaacagctg
     5101 caaaacaccg gcatcgaaca ggatatcctg tgcagcgagc acggtcggcg accccatcgg
     5161 accgaccatg gcaaatatct tgtcgcgctc gatcatcttc tgcgacgcga gcaccgcacg
     5221 tttcggatcg tagccgctgt cctcgaggat catcctgatc ttgcggccat tgatgccgcc
     5281 ggccgcattg atttcctcga cggccatttt catgccgttg gaaaccggaa cgccccagac
     5341 cttgatcggg cccgacaagt cctgatgggt accgatcacg atctcggtcg ccgagatgcc
     5401 ttcattggtg accttggttt gtgctgatgc cggcagatgg gtcagcgcaa gggcgcccac
     5461 cgcaaggccc agcgccttga acgatttcga catgtcagtc tcctctccgt tggcccgtgt
     5521 tgagcgaagg gtgggccttc aatccttcgc caatttcaag agcgctcgcg ctgttcaagc
     5581 caccgcccgg tcgccgtaca tggcgttgat ctcgatcgcg tagcgcttat tcacgaaatt
     5641 gcgcttcagc ttcatggtcg gcgtcagctc ctcgtcttcg ggggtcagct ggcgttcgat
     5701 caggaagaac ttcttgatgg tttcgacccg tgcgaaattg gcgttgaccg cctcgatctc
     5761 gcgctggatc aaatcctgga tctccgccgc gcggcacagg ctcgcataat tggtgaaggg
     5821 aatgtcatgg tcctgggcga acttctcgac attctcctgg tcgatcatga caaggcaggt
     5881 cagatagggg cgcttgtcgc cgatcacgac agtgtccgag atgtagggcg agaatttcag
     5941 ttggttctcg atctgcgaag gcgtgatgtt ctttccgccc gaggtgatga tgatgtcctt
     6001 catccggtcg gtgatcttga cgaagccctc attgtcgatc gagccgacgt cgccagtgtg
     6061 cagccagccc ttggcgtcga tggtctcggc ggtcctttcc ggctgattga gatagcccat
     6121 gaacaggaag tcgcccttga tcaggatttc gccttcgggc gagattccga cttctcccca
     6181 gggtgctgct ttcccgaccg agccgagctt gatgcgatcc ggcggcatga tggtggcaac
     6241 accgcagttc tcggtctgac cgtagacttc gcggatgtcg atgccgagcg cgagatacca
     6301 gcggatcaga tccggtgcga tcggcgccgc accggtaaag gccagacggc aacgatcgag
     6361 accgagcatg cacctgatgt tgcggaacac cagccagtag gcggcgcgat tgacgagacg
     6421 caacgacagc gaaggcgtct cgccgtcgag cctgcaatcg gtcatgcgat agcccaccgc
     6481 cagagcgtgg cggtacatcc agttctggaa cggggttgcg tccttcagcg cgatcgtgat
     6541 gccggaatag aacttttccc agatccgtgg taccgccaga aacgctgtcg gctgcacttc
     6601 gcgcaggttg tcgggtaccg tctccgggct ttcggcgaaa ttcatcaccg accccagtgc
     6661 cagcgagatg taatagccgc cgatccgttc ggcgacgtgg cagagcggca ggaacaccag
     6721 acgctcttcg ctgtcggtag agggaaacag atcgttggcg tggcgcatct gatgggtgac
     6781 gccgcggttg gaatgcatgg cgcccttggg cgggccggtg gtgcctgagg tgtagaccag
     6841 gatggcgaga tcgttggcac cacggctcgc aaccatctcc tcccacaacg cttcgcggcc
     6901 ctggatgtgg ttgcgtccga gtgctgtgaa ttcggcaagc gacatcacca tcgaatcgga
     6961 aaagccgctc aggccctcca tgtcgaagac gacgatcctc tcgagcgtcg gacagcgcgc
     7021 acggcacgac aggatcttgt cgagctgctc ctcgtcctcg gcgaagatca cccgggtgct
     7081 ggagtcattg acaagatatt ccacctggct gctggaatcg gtcggataga ttccggatga
     7141 aacgccgccg gcgcacagga tgcccatatc ggcgaatacc cattccggta ccgcgttcgc
     7201 gacgatcgag gcgacgtccc ccggacggaa gccgatggcg tgcagaccgt aggcgatttc
     7261 cttcgatatc tgcagccact gccgccaact ggtcggctgc cagacaccga atttcttctc
     7321 gcggatcgcc gtccggtcgc cgcgcgtttc cgcggcaagc agaaaactgt tcgcgatcgt
     7381 gtcggccacg gtcagcactg ccggtctggc catgctcgtt ccctccctcg tctcgcccgc
     7441 gtcggatgcc ggctttgaca gccggtcttt tttccgaagc agaacccgta tttagcgcca
     7501 agtctttctt aacgccaagt tgtctttctt aacgccaagt tttcttcctt aacgccaagt
     7561 tttcttcttt ttccagcgcc gttcgccccg cgcgccggct tccttggcgc cgagatagaa
     7621 ttcctgaatg tccttggagt gcagcagccg ctcgcaactg tcgttcatga ccacccggcc
     7681 gatttccaga acgtaaccat aatgcgccgt ctccagcgcg accttggcgt tttgctcgac
     7741 cagcaggatc gacatgccct gctcttcatt gacgcggcgg atgatcgtga agatctcctt
     7801 caccaggatc ggcgacagcc cgagcgaggg ttcgtcgagc agcaatagcg tcgggcggtt
     7861 catcaacgcg cgcccgatcg cgagcatctg ctgctcgccg ccggaaagct gaccggcggg
     7921 ctggttgagc cgttccttaa gccgcggaaa atatccgtag acccgctcca gatcctcagc
     7981 gacatcatcg cgatctttgc gcggataggc gcccatcatc aaattctcgc gcacgctcag
     8041 gaacggaaat acctcgcgcc cctcgggcac atggcttaag cccagccgca cgatcttgtc
     8101 ggcttccatg cgctggatcg gcttaccgag aaattcgatt gagcccttct gcggatcgag
     8161 aatgccggag atcgtcttga gcaccgtggt tttgccggcg ccgttggcgc cgagcagggt
     8221 tataatcttg ccgcgcggca cctcgatact gatgcctctg atggccatga tcggcccgta
     8281 gtagctctcg atgttgctga gtctgaggat gatgtcggga gcgctcatgc cgtcatcctt
     8341 caggtgccga gataggcggc aacgacgtcg ggatgacgct gcacttcggc cggcgatcca
     8401 attgccagga ccttgccgta gttcaccgcg atcacgcgat cggaaacacg attcaccagt
     8461 gtcatgtcgt gctcgaccat cagcacggtg atgccaagct cggtcctcat gtcgcggatc
     8521 cagaacgaca tgtcgtcggt ctcctcgaca ttcagtccgg acgacggctc gtcgagcaag
     8581 atcagtttcg gctcggtgca cagcgcacgg gccagctcga tgaccttgcg cacgccatag
     8641 ggcagtcccg agatcagctt gtcgcggtag ggctcgagat cgagaaattc gatcacttgc
     8701 tcgacgcggc ggcgatggat tttttcgttg ctgcgcacgc tcggcagaaa caacaactcc
     8761 tgccagagct gcgtggtgga atgccggtgc cggccaacca gaagattgct gagcaccgtc
     8821 gcattctcga acagttcgat gttctggaag gtgcgggcga tgccgagctt ggcgatgccg
     8881 aacgccggct cctgggtgat gtcctgatcc tcgaaataaa ttcttcccga ggtcgggctg
     8941 tagatccgcg agatcaggtt gaagatcgag ctcttgccgg cgccgttggg cccgatgatc
     9001 gacaggatct cgcccttctc cacggcgaag ctgaccgcgt cgaccgcttt caggccgccg
     9061 aaatgcaacg acagattttc ggcgcggaaa tagctcaccg gtttcgctcc gatttcacat
     9121 agatcttctg ccgcttgaag gtggcgcgct tgtagagcgg gaacagctgg aagaagagct
     9181 ttatcttcag ccagcggccg taaagaccga gtggttcgaa cagcacgaac agaacgatga
     9241 tgatcccgta gatcgcgcct ttcaggccgt tggcggaagc gaacgcggcg acgttgctct
     9301 ggatgtgcgc cacgctctcc ttccccgcgc cgagcgtagc ggcgacgccg gcgaccatgc
     9361 cgggaacatc gtccttgagg taggtcagga acggatcgat catgacgatg aagatcgccc
     9421 ccagcacggc gccgtgcggg ctgaatgcgc cgccgatcag gatcacgatg atgaactcga
     9481 tcgagagctg cagcgtaaac atttccggac tgatgaagga cagcttgtgg gcaaacaggc
     9541 agccggcgaa gccggtgatg ccagcgctga tcgcgaatga cttgaccttg tagagcgata
     9601 cgttgacgcc catgcttctc gcggccgtct cgctgtcgcg gatcgcgaca aaggcgcggc
     9661 cggtcggcga acgcaagaga ttgagcgtgc cgacgatggt caggatcagc acgccaagac
     9721 aaaggaaata gaaagccgga ctgtcgcgcg gcacagacgt gcccagcaac tgaatggtct
     9781 tgacgcgcat gccctcgttg ccgtgcgtca cgctttccca gcgcgccagg atttcctcga
     9841 cgatgaaggc aaaggaaatc gtcgcgatga cgagatagat cccttgcagc cgcaacgccg
     9901 ggaatccgac cagcgcgccg ataatcccgg tcaacaggcc ggcggcgagg aagtagaccg
     9961 gaaacggaac gttgaattgc tgcaaatagg cggcggtata ggcgccgatg gcgagaaagg
    10021 cggcgtggcc caatgacgcc tgcccggtga acccggtgag gatcatcagc ccgacgccga
    10081 ccgtggcgta gatgcagacg aacaccagtt ggctgacgag ataactggag aggacgaacg
    10141 gcgcgatcac aagaaccgca agcagaatgc cgtaggagac cacatagccg gagtgcgaaa
    10201 acagcctgat gtcgtcttca taatcggtct tgaacatgaa gcgcatggct aaatcctaga
    10261 ccttcttgcg catgcgaagg ccgaacaggc cttcgggctt gagcagcagc accaccagca
    10321 gaacgatgta gggtgcgacg tccttccacc cttgcggcag atagaagccg gccatgctct
    10381 cgatcacgcc gatcagcacg ccgcccacga ccgcccccgg gatcgagccg aagccgccga
    10441 gcacggccgc cggaaacgcc ttcaggccga gcaccagacc gacgttggaa tgaatgaagg
    10501 tgatcggcgc cagcagcacg ccggcgcagg tcgccaccgc ggcgctgatc gcccacacga
    10561 tcgacaccac gcgcttgacc ggaatgccca tataataggc ggccagcatg ttctccgaac
    10621 tggcgcgcat cgcggtgccg agcgtggtct tgttgaagaa cagatagagc agcgcgcaaa
    10681 ggatgatggt cgcggcgatg acggagagct tgtcataggc cagcaccagg ctgccgatcc
    10741 gcaacacgcc ctggctgaac ggggtctcga tcttgaagtc atcggtgccc cagatcatgc
    10801 cgaccacgga gcgcagaaaa tatccaagcc cgatcgtcgc catgatgatg gaaaactgcg
    10861 gatagccgag gatcggacgc accaccaggc gttcggccag catgccgaac agcgccatcg
    10921 ccgcgaccgc gccggcaaag ccgatccagt aattgagccc gagaatgccg atgaaggtga
    10981 aagcgaaaaa tccgcccagc atcatcaggt cgccctgcgc gaagttcacg acttcggtcg
    11041 ccttgtagac aaggacgaag ccaagcgcga tcaggccgta aacgcagccg agcgcaatgc
    11101 cgctcaccaa ctgctgaaca aagtccagca ttgccgacac ccctcccccc gatgcgcggc
    11161 gattctagcg atcgcttttc ccgcatcttg tgtccacgcg ctgcccgccg ccgcttgcgc
    11221 cgaaaagccg catgaccaat tggtggttat acagcccaat gcgccgatcg ctgtcaacaa
    11281 agcgctggtt gccaaccgca tcccgagttg agcacaaatg ccgcagcctt gaacgatcag
    11341 cgttgaagga tcagccttgg actatcggcc ttgggctatc ggccctgcgc caatcccggg
    11401 cattggcggg atttgccgcg cccgattcac cgtgccgaaa tatcttgggt ccatggttgg
    11461 tttcgaaacc aaaccaacga ttgtttcga
//
LOCUS       DASWGM010000173         7909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_846356,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000173 DASWGM010000000
VERSION     DASWGM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846356"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VGA15_05260"
     CDS             <1..613
                     /locus_tag="VGA15_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9587135.1"
                     /translation="STLLTRLAHGLLDVVLLALPIDKLELETLTLFDDRFLLAVPARD
                     PLPERSRVTTRDVNARKLVLLEEGHCLRDQALIYCARRSDADSNLGATSLATIIQMVA
                     SGYGVTLLPEVAIDAEVRDDRVKLLRFVEPQPKRSIGLAWRSTSPRKADFLALGEMVV
                     EALKVQGAKSASASRLPDVKARPAAATNTPRPGQGSSTRNLNG"
     gene            1249..2238
                     /gene="dusA"
                     /locus_tag="VGA15_05265"
     CDS             1249..2238
                     /gene="dusA"
                     /locus_tag="VGA15_05265"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX9587136.1"
                     /translation="MLEARFAIAPMMDWTDRHCRVFHRLMTRRARLYTEMLTTGAIIH
                     GDRRRLLGFDACEHPVALQLGGSDPRDLAAAAKIGEAFGYDEVNLNVGCPSDRVQDGR
                     FGACLMAEPALVAEGVDAMKRAVDIPVTVKCRIGIDDQDPEIALDALARGVIAAGADA
                     LIVHARKAWLSGLSPKQNRDVPPLDYDIVYRLKAALPDVPIIINGGIGNLGEAKAHLG
                     FVDGVMLGRAAYQEPWRLLGADPELFGEAAPHPTMKHVFEAMVPYIESQLAQGTRLHS
                     ITRHFVGAFHGVPGARAFRRHLAENGVKPGAGVNVLRDAIALVEDSVVAPIAA"
     gene            complement(2385..3110)
                     /locus_tag="VGA15_05270"
     CDS             complement(2385..3110)
                     /locus_tag="VGA15_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587137.1"
                     /translation="MEMIKCISVIAASALGLISLVLPVAAEPPTKIDFSAETVGAEPK
                     SLVAVVGIWRIESEGGKNVLAVDGRQWKEGQSSAGIADKARALYGERYAEFLDRVQAY
                     AYYPYTVTRDTENFRGGEIAVRFEGLSGRIDQGAGILFNLKPNGDYLALRANCLENNL
                     VLWKFEKGKRSSVAWIRNAPAASKQWHDLKVRVSGTKVEGYLDGKLYLEHTLPEAVSG
                     RVGLWSKADSYMHFSDYTVTPPN"
     gene            complement(3124..4488)
                     /locus_tag="VGA15_05275"
     CDS             complement(3124..4488)
                     /locus_tag="VGA15_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9587138.1"
                     /translation="MHSPETFHVQNAPLRPLLVKELWEILSGRALWTMLLLMCPLLGY
                     SFLQATSLYGEASVAGLQSPVLASSLSPLDGVLVPTLGASYVAVTLLFPFVAIRVLGQ
                     EKESGALRLLVQLPYRPPALIAAKLAAVLAALVLSSIPAFSALVIWEMQGGHLSAPET
                     LNLLFGHLLYGLLVGAIALFAASVSESAATAAIVTLAFTIGSWVLDFTVAGQPGLLAW
                     IARLSLTQILRTFEQGLLSAGLVLGIGAAMFGFAALANVWLPPGVQVRDKVIRSIAWV
                     LVVALVLGLATQIKLTFDVTEDRRNSFPAADQHLLASLAQPLVVTVNLAPEDPRYADL
                     QRNVLAKLERAMPNVSIRLASGRQSQAATAGEDRYGEVEYAYGARSDVSRSTSPREIL
                     PMLYGLAGVSPPAPAPGSDYPGYPLVAGRRVALLWFFAGLPLCIALAWRWNRRLPKPI
                     TARS"
     gene            complement(4475..5233)
                     /locus_tag="VGA15_05280"
     CDS             complement(4475..5233)
                     /locus_tag="VGA15_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587139.1"
                     /translation="MDGVLPIVAASAFGQARDPVLLRVDSITKQYADQAVLSDVAFDV
                     QAGEILGIIGPNGAGKTTLLEALAGVLPAETNDVRWRGERLPASRRREAMFYLPDGVR
                     PYLDQPVTRVLSFIAGVYRRSTGEIADAIKSVGLTPVLHKRVHSLSKGYGRRLMLALG
                     LLTPHPLLLMDEPFDGFDLRQIRTIVDVLRREASEGRTLALAIHQLPDAERVCDRFIL
                     LADGRVRGVGTLNDLRARTGISEGSLEDIFLALT"
     gene            complement(5409..5921)
                     /locus_tag="VGA15_05285"
     CDS             complement(5409..5921)
                     /locus_tag="VGA15_05285"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX9587140.1"
                     /translation="MSRILLVTLMLAATVGAVVAYADPEQIARASDNVSELMRRRAAS
                     PARAVQIPRGQTGEFALHAKINGVNAPMVIDTGATSVVLTWETAKAAGLPLELLEYDV
                     DVETAGGHTRAARLTLNRLAIGKLVERSIPALVVPRGQMKTNLLGMSFLDRLESWEVR
                     ADRLMLRGYP"
     gene            complement(5953..6162)
                     /locus_tag="VGA15_05290"
     CDS             complement(5953..6162)
                     /locus_tag="VGA15_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX9587141.1"
                     /translation="MSKETPCIAVCMIDPKTSLCFGCGRTLPEIARWHRMESAERHAV
                     MAQLAARMADAGLVQPGMAASPKPE"
     gene            complement(6208..7800)
                     /locus_tag="VGA15_05295"
     CDS             complement(6208..7800)
                     /locus_tag="VGA15_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9587142.1"
                     /translation="MSNPADKPEVFQLPAETSIAPPVSNRRAAAQRVREARDRLTSTS
                     GTRPAFDRELLRQHAQTRISASYVVMLLVVATGLLFGFWMHPISAAAWTCGMLCIHAA
                     IIRNCVRFLGEPPSLTATRKWRTRFVLLDLLYGLCWTAILTHPASIDAVSNTLMMFLM
                     LLVIAVSSMLAASLPIAALAATVPVTSAIALNFALAATFDNYVLAVLALIAEGYFALL
                     ARRLHSTTLATLEARAEKDALIGELEQAKSISDEARHRAESANVAKSRFLAQMSHELR
                     TPLNAILGFSEVMKSEIFGPHAVPVYKEYSADIHNSGVHLLNLINEILDLSRIEAGRY
                     ELNEEAVSLVHVVADCHHLLKLRASSRGITIHEVFEQGMPRIWGDERATRQIVLNLLS
                     NSIKFTPQGGEIWLKVGWTASGGQYLSVKDTGSGIAEDEIPIVLASFGQGSNSIKSAE
                     QGAGLGLPIAKSLVDMHGGTFTLKSKLRIGTEVIVTFPPERVMSALAPMAEEAPPLQP
                     EATIDERHRARNKPIMSAGTGL"
BASE COUNT         1597 a         2393 c         2444 g         1475 t
ORIGIN      
        1 cagtacgctt ctaacgcggc tcgcacatgg gttgctcgac gtggttctgc tggcactgcc
       61 gatcgacaag ttggagctcg agacactcac cctgttcgac gaccggttcc tgctggcggt
      121 gcctgcccgc gaccctttgc ccgagcgcag ccgcgtgacg acgcgtgatg tcaacgcacg
      181 taaattggtt ttgctcgaag aagggcattg tctgcgcgac caggcgctca tctattgtgc
      241 gcgccgcagc gatgccgatt ccaacctcgg tgctaccagt cttgcgacga tcatccagat
      301 ggtggcgagc ggctatggtg tgacgctcct gcccgaggtc gcgatcgacg ccgaagtgcg
      361 cgatgaccgg gtgaagctgc tgcggttcgt cgagccgcag cctaagcgaa gcatcggcct
      421 cgcctggcga tcgacttcgc cgcgcaaagc cgacttcctg gcgctcggcg aaatggtggt
      481 cgaggcgctt aaggtccagg gagcgaaaag cgcgtcagcg tcgcgcttgc cagacgttaa
      541 ggcgcgtcct gccgccgcga caaacacccc ccgcccgggc caaggttcga gcacccgtaa
      601 cctgaacgga tagcccgcaa gtgccccacc gatcgtcggg acgacgagga acatccagag
      661 ctgcgccggc tggttcggtc gcgggcggcg cttgaggcag caaataaacg ttctgcggca
      721 gacggctccg gaaaaaccct ccttcagcgg cttcggacgc tgcggtcatt ctttaaatag
      781 ccaccatcag tgaatgtcgg tctcggtggt tgcgcctccg cgatttgaac ctgccgccgc
      841 caactcgcag cagcatggcg gcgccatccc gccgcatgca agttctttta ctcggcgatc
      901 gccgaaacta cgatccgagc attgcgggcc taggcacggg taaggcgcag cgctactttg
      961 cttctgactt tgatcggctt tcggattgct tgttcgacgc tagcggaatc gaagcgatcg
     1021 aagtcgtaat gagtagtgtg cagcaacgcg gtactgcatg cgttcggcca gtacagcaat
     1081 gcagtactgg atcgaggaag aatcaacgaa ggcgaattac atgaagaagc cagaaatggt
     1141 ttaaccagtt gaaaagaaag gaaaatgctt taggtctatc gctccgatca tttgtcggcc
     1201 caaaaatcgg tctaacggac tcgtgttacg aaattcaatg aaaacaatat gttagaagcc
     1261 agatttgcca ttgcacccat gatggattgg acggaccggc attgccgcgt cttccaccgc
     1321 ctgatgacgc ggcgcgcgcg gctttacacg gagatgctga cgaccggggc catcattcat
     1381 ggcgaccgcc ggcgtctgct tggtttcgat gcatgcgaac atccggtggc gcttcagctt
     1441 ggtggttccg acccgcgcga tctggcagca gctgcgaaaa ttggcgaggc gttcggctac
     1501 gacgaagtca atctgaacgt cgggtgcccg tcggatcgcg tgcaggacgg ccggtttggc
     1561 gcctgtctga tggcggagcc ggcgctggtc gccgagggcg tcgatgccat gaagcgcgcg
     1621 gttgacattc cggttacggt caagtgccgg atcggcatcg acgatcagga tcccgaaatt
     1681 gcgctggatg cgctggcacg cggcgtgatc gcggccggcg cggatgcgct gatcgtgcac
     1741 gcccgcaaag cctggctcag cggattatcg ccgaagcaaa atagggatgt cccgccgctc
     1801 gactatgaca ttgtctaccg gctgaaggcc gcgttgcccg acgtgccgat catcatcaac
     1861 ggcggcatcg gaaacctcgg cgaggccaaa gcgcatctcg gctttgtcga cggcgtgatg
     1921 ctgggacgcg cagcctacca ggaaccatgg cggttgcttg gcgccgatcc cgaactattc
     1981 ggcgaagcgg cgccgcatcc gacgatgaag catgtgttcg aggcgatggt gccctatatc
     2041 gaaagccaac tcgcgcaagg cacgcggctg cattcgatca cgcggcattt cgttggcgca
     2101 tttcatggcg tgccgggcgc gcgcgccttc cgccgccacc ttgccgagaa tggcgtcaag
     2161 ccaggggccg gcgtgaacgt cttgcgcgac gcgattgcgc tggtcgagga tagtgttgtc
     2221 gcccccatcg ccgcgtgatg tcgaaaccaa aaattttgcg cgtattcgga tcgcaaaacc
     2281 gaaaatccgc cttgatcgcg aatccgtcgc cgaagccgcg cgcatacaac aggaagacat
     2341 ccgctcgtct tccgatagcg gcttttctgt tgaaccagcg acagtcaatt gggcggagtc
     2401 acggtatagt ccgagaagtg catgtagctg tccgccttgg accacagacc aacccggcct
     2461 gacacggctt cgggaagcgt gtgctccaga tagagcttgc catcgagata gccttccacc
     2521 ttggtgccgg aaacgcgaac cttaaggtca tgccattgct tgctggcggc cggggcgttc
     2581 cggatccagg ctaccgatga gcgtttgcct ttttcgaact tccagagaac cagattgttt
     2641 tccagacaat tcgcacgaag tgccagataa tcgccgtttg gcttcagatt aaacaatatg
     2701 ccggcgccct gatcgatgcg gcccgatagt ccttcgaacc gcacggcgat ctcaccgcca
     2761 cgaaaattct ctgtgtccct agtgacagtg taggggtagt acgcataagc ttgaacccga
     2821 tcgagaaatt cagcatagcg ttcgccgtag agcgctcttg ccttatcggc gattccggcg
     2881 gaggactgcc cttctttcca ttgccgccca tcgacggcca ggacgttttt cccgccttcg
     2941 ctttcaatcc gccagatccc gacgacggcc acaagcgact taggctccgc tccaaccgtc
     3001 tcggcagaga aatcgatttt ggtcggcggt tctgccgcga caggcaatac caatgatatg
     3061 aggccgagcg ccgaggccgc gatgacgctg atgcatttga tcatttccat gggtgaacct
     3121 ccttcatgat cgtgctgtga ttggtttggg tagacgtcga ttccaccgcc aggcgagggc
     3181 gatgcagagt ggaagtccgg cgaagaacca aagaagcgcg acccttctgc cggcaaccag
     3241 cggataaccc ggataatcgc tccccggggc aggcgcgggg ggactaaccc ctgcgaggcc
     3301 gtacagcatg gggaggattt cccggggact ggtcgagcgg ctgacgtctg atcgcgcgcc
     3361 ataggcgtat tcgacctcgc cgtagcgatc ctcgcccgca gtggctgcct ggctttgccg
     3421 cccgctcgcc agtcggatcg acacgttcgg cattgcgcgt tcgagcttcg ccagcacgtt
     3481 gcgctggagg tcggcgtagc gggggtcttc aggtgccaga ttaaccgtca cgaccagggg
     3541 ctgcgccaaa gatgcgagca ggtgctggtc ggctgcggga aaagagttgc gtcgatcttc
     3601 ggtgacgtcg aacgtgagtt tgatctgcgt tgcgaggccg agcacgagcg ccacgacaag
     3661 aacccacgcg atcgagcgaa tgactttgtc gcggacttga actcccggcg gcagccaaac
     3721 gtttgccagc gcggcgaaac cgaacatcgc cgcgccgata cccaggacaa gcccagccga
     3781 caagagacct tgctcgaacg tacgcaatat ctgggtcagc gacaggcggg caatccatgc
     3841 gagaagcccg ggttggccgg ccacggtgaa atccagcacc catgaaccga tcgtgaaggc
     3901 gagcgtaacg atcgcggcag tcgctgcgct ttccgacacg gacgcagcaa acaaggcgat
     3961 agcgccgaca agcagcccat agagcaggtg tccaaaaagc aggttcaacg tttccggcgc
     4021 ggacaagtgc ccaccctgca tttcccagat aaccagtgcg gagaacgcgg gaatgcttga
     4081 aagcacgaga gcggcgagta ccgccgcgag cttggctgca atcaaggcag gcggacgata
     4141 gggcagttgg accagaagac gcagtgcacc cgattccttt tcctgaccga gcacacggat
     4201 agcaacaaag ggaaagagca gcgtgaccgc gacataagag gctcccaggg tcgggaccag
     4261 cacgccgtcc aatggcgaca ggctgctcgc gagcactggt gattgcagcc ccgccacact
     4321 ggcctcgccg tacagcgaag tggcctgaag gaaactgtat ccgagcaaag ggcacatcag
     4381 cagcagcatg gtccaaagcg cccggccgct gaggatctcc cacagttcct tgacaagcag
     4441 tggccgcaag ggcgcgttct gaacgtgaaa tgtctcaggt gagtgcaaga aagatatcct
     4501 ccaggcttcc ctcggaaatt ccggttcggg cgcgcagatc attcagtgtc cccacaccgc
     4561 gaactcgacc atcggcaagg agaatgaagc ggtcacagac gcgctcggcg tccggcaatt
     4621 gatgtatcgc gagggcaagc gtccgcccct cgctggcttc tcgacgcagc acatcgacga
     4681 tcgtgcgaat ctgccggagg tcaaagccat cgaacggttc gtccatcagc agcagcgggt
     4741 gcggcgtaag caatcccagc gccaacatca gccttcggcc gtagcccttc gacagtgagt
     4801 gcacgcgctt atgaaggacc ggcgtcaaac ccaccgattt gatggcatcc gcgatttcgc
     4861 cggtcgatct ccgatagacg cctgcaatga acgatagaac ccgggtgacg ggttgatcca
     4921 ggtaaggtcg cacgccatca ggcaggtaaa acatggcttc gcgtcggcgt gaggccggca
     4981 agcgctctcc gcgccatcga acgtcgttgg tttccgcagg gagaacgccc gcgagggcct
     5041 caagcaaggt tgtcttgccg gcgccgttcg gaccaataat accgaggatc tcacctgcct
     5101 ggacgtcgaa ggcgacatcc gagagtacgg cctgatccgc atattgcttc gtgatcgaat
     5161 cgacacgtag cagaaccgga tcgcgcgctt gtccaaaagc ggacgcagct acgatcggga
     5221 gaactccatc catgcgcaat cccctcgacc tgggcgagtg ttgcgcagag cttggacggc
     5281 gagcgtctga gggggagtct gcctgtcccc gatcagagat tagtcctcga tccgtccgac
     5341 tacaaggacc cggcacaggg gctctgtcga cgataccccg gctcttcggc ttttgcggaa
     5401 tacgcggctc agggatagcc gcgcagcatc aacctgtcgg cgcgcacttc ccagctttcc
     5461 agccgatcca gaaagctcat gccgagcaga ttggtcttca tctgtccgcg cggcaccacc
     5521 agtgccggta ttgaacgctc gacgagcttg ccgatcgcaa gacgattgag cgtcagccgc
     5581 gccgccctgg tgtgtccgcc agcggtctcg acgtcgacat catattcgag cagctccagc
     5641 ggcagcccgg cagccttcgc cgtctcccag gtcagtacga cggaggtcgc gccggtgtcg
     5701 atcaccatcg gggcgttcac gccgttgatc ttggcatgca gcgcgaactc gccggtctgc
     5761 ccgcgcggga tctgcacggc gcgcgccggc gacgccgccc gcctgcgcat caattccgag
     5821 acgttgtcgc tcgcacgcgc gatctgttcg gggtcggcat aggcgaccac ggcgccgacc
     5881 gtcgcagcga gcatcagggt gacgagcaga atgcggctca tggctcgcct cctgtgcaaa
     5941 tggtgtcgcg ggtcattcgg gttttgggct ggccgccatc ccgggctgaa ccaggccagc
     6001 gtcggccatc cgcgcggcga gctgcgccat cacggcatgg cgctccgcgc tttccatccg
     6061 gtgccagcgc gcgatctccg gcaatgtacg tccgcagccg aaacagaggc tggttttggg
     6121 atcgatcata cagactgcga tacacggcgt ttctttgctc attgagagag tgtgaaatgg
     6181 tttcggtggt ttgcgcaagc gctccagtta caatccggtc cccgcactca tgatcggttt
     6241 gttgcgcgcc cggtgccgct cgtcgatggt ggcttccggc tgcagcggcg gagcctcctc
     6301 ggccatcggc gccagcgcgg acatcacgcg ttccggcggg aaggtgacga tgacctcggt
     6361 gccgatgcga agcttcgatt tcagcgtaaa tgtgccgccg tgcatgtcga ccagactttt
     6421 tgcgatcggc agcccgaggc cggcaccctg ttcggccgac ttgatcgagt tggagccctg
     6481 gccgaacgac gccagcacga tggggatttc gtcctccgca atgccggagc cggtatcctt
     6541 gacgctcaga tattgtccgc ccgacgccgt ccatccgact ttcagccaga tctcgccgcc
     6601 ctggggcgtg aacttgatgg agttcgacaa gagattgagc acgatctgcc gcgtcgcgcg
     6661 ctcatcgccc caaattcgcg gcatgccctg ctcgaatact tcgtgaatgg tgatgccgcg
     6721 actggacgcg cgcagtttga gcaaatgatg gcagtcggcg acgacgtgca ccagcgacac
     6781 ggcttcttcg ttgagttcgt atcgtccggc ctcgatccgc gacaggtcga ggatttcatt
     6841 gatgagattg agcagatgca cccccgaatt gtgaatatcg gccgaatatt ccttgtaaac
     6901 cgggacggcg tgggggccga aaatctcgct cttcatcacc tcggaaaacc cgaggatcgc
     6961 attgagcggc gtccgcagct cgtggctcat ctgggccaga aaccgcgact tggcgacgtt
     7021 ggccgattcg gcgcgatgac gggcctcgtc ggatatcgac ttggcctgct cgagctcgcc
     7081 gatcagcgcg tctttttcgg cgcgcgcctc caatgtcgcc agcgtcgtcg aatgcagacg
     7141 acgggcgagc agcgcaaaat aaccttcagc gatgagcgcg agcaccgcga gcacataatt
     7201 gtcgaaggta gcggccagcg cgaaattcag cgcaattgcc gatgtgaccg gtacggtcgc
     7261 cgcaagcgcc gcgatcggca ggcttgccgc gagcatgctc gacacggcga tcaccagcag
     7321 catcaggaac atcatcagcg tgttcgagac cgcatcaatg ctggcgggat gggtgaggat
     7381 cgcggtccag cacagcccgt acagcagatc gagcagaacg aatcgcgttc gccatttgcg
     7441 ggtggcggtg agcgagggcg gctcgccaag gaacctgacg caattgcgga tgatggcggc
     7501 atgaatgcac agcatgccgc aggtccacgc cgcggccgag atcggatgca tccagaaccc
     7561 gaacagcaag ccggtcgcga ccacgagcag catcacgaca tacgatgccg agatgcgcgt
     7621 ctgggcgtgt tgccgaagca gttcgcggtc gaaggcgggg cgggttccgc tggtcgacgt
     7681 taaccggtcg cgggcctcgc gcacccgctg cgcggctgca cgacggttac tgaccggcgg
     7741 tgcaatcgac gtctcggccg gaagctggaa gacttcgggc ttatcggcgg ggttactcat
     7801 caaacaacac aaatcctgcc cgcgaacggc gcgggctttt gcattgtcta tctctggcgc
     7861 caaatcatta agagctgcct taaagagcta aagaatcacg ttaacggga
//
LOCUS       DASWGM010000174         5202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1535402,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000174 DASWGM010000000
VERSION     DASWGM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535402"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..893
                     /locus_tag="VGA15_05300"
     CDS             477..893
                     /locus_tag="VGA15_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587143.1"
                     /translation="MSPIRSILSIVLVTAWSSASAETMKFEDAAGILGASCAKEIDTN
                     CRGVSLDPARLKDCLLRNQDSLSAQCRTDYVRAFDAIQKRVAARSAVAKICERDAVKL
                     CGGIQKDGVQALECILTAPRGLSARCNQAIGEAGYR"
     gene            929..1498
                     /locus_tag="VGA15_05305"
     CDS             929..1498
                     /locus_tag="VGA15_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9587144.1"
                     /translation="MFIVLLIGSMAPLSSARAQASAPTEDWVKQLAGLEPAPDMEIAA
                     LRQQALERVKSKADGVPLQRPPVAAQLLKLPRLAVEIQFDEDSSIIRPESYRTLGRIA
                     DTLYDPALLGYKFLIVGHTVATGKREYNLTLSQKRADAIRGALVTAFKISPKRLQGIG
                     LGEEQLLDAAHPTAPINQHVEVATLGKAF"
     gene            complement(1507..2511)
                     /locus_tag="VGA15_05310"
     CDS             complement(1507..2511)
                     /locus_tag="VGA15_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9587145.1"
                     /translation="MTTVGRAVSRLRLAIFPAVFALSALAASHHEARAEASYPNKSVR
                     IVLPFAAGGVADTTARIVAEKLGDKLGQRFYVENQAGAGGIAAARTVIASAPDGYTLA
                     LLSNGTAVSVSLFKKLPFDPLRDFEPISSLGFFDFVFATGANSELRTLADFIAAAKAK
                     PGALNVGTINIGSTQNLSAELFKTSADIDFTIIPHRGTPEVQISLLQGDIALVIDSYS
                     SMKGNLDDRKFRALASSGPVRSKSTPAVATVQESGVANYDVVSWNALFAPTGTPPEIV
                     NKLNGALQDILADADVKKRLIELGIEARASTPQEISARLKSDIDKWQKVIEKAGIQKQ
                     "
     gene            2772..3086
                     /locus_tag="VGA15_05315"
     CDS             2772..3086
                     /locus_tag="VGA15_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587146.1"
                     /translation="MNILSISLAVGASAFFMTAASAAPLSAGASIAPESNIQNVRMVC
                     NENGRCWRERSERRVIIREPRDSYGYAPREGYVERRGYDEDRGIGFRAPGVSIGIGTE
                     RY"
     gene            complement(3606..5165)
                     /locus_tag="VGA15_05320"
     CDS             complement(3606..5165)
                     /locus_tag="VGA15_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEX9587147.1"
                     /translation="MSLFSDPRWSEALGHLPDYLGNHVRVSVTALALGLLVSLPLAIF
                     SRNRPVMRGALLGLASIVQTVPGLALLALFYPLLLALAQLSLAWFGVGFSAFGFLPAV
                     LALALYSMLPVLRNTITGLQGVEPAILEAAQGVGMTERQSLLSVELPLALPVMMAGIR
                     TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWVFVLFGCLAAAVLALAVDQLLALI
                     ETGLRQRSRLGVAFGGVGIAVLVAATLVPAVTRSQSTYIVGAKTFAEQYVLSALIAQR
                     LRAAELSASSREGLGSNVIFDALASGDIDVYVDYSGTLWANQFHHSDIKPREELLDEL
                     KATLATQKITLLGELGFENAYALVMPRKRAEELGIHSIADLASHAPAMSMAADYEFFS
                     RPEWAGLREAYGLSLRAQRQMQPDFMYAAVASGEVEVIAGYTSDGLIAKYDLVVLDDA
                     KHAIPPYDAIVLLAPKRASDQALRAALSPLLGQIDIATMREANLRAAGGDGVSSPDAV
                     ARWLWEKIGKR"
BASE COUNT         1023 a         1566 c         1595 g         1018 t
ORIGIN      
        1 tccaggaagc cgccggcatt ccgctcgatc cttcgttcga ggagcagaag aacatcatgc
       61 tgcgctgcaa gggtgttttc tatgcctgca aggacggcgc cgaagcccgc cgcttcaacc
      121 ggctcctgat cgatgccggc ctggtgcacg gattgtaggg ccgcgggagt ccggttcccg
      181 acgaaagcag aaatgccgcg gccggcttga gcgcgcgtcc aactctgcta tgatccagct
      241 attgcaagcc tgacgttttt gccgccatgc tgatgcgtct gttcgcaccg caatttcttg
      301 tggcctgggc ctttctcggc ttggccgtcg cggttcattt ccgcggcaag gttcggctcg
      361 gattctggcg gcaggtctcc tgctgatcgg tggcatactc gcagcattcc tggcgacggc
      421 gttagcctga ggccgggcct tgagcgcctg atccacttaa aggcgcggat gagaccatga
      481 gcccgatccg atcgatatta tccattgttc tggtcaccgc ctggtcgtcg gcgtcggcgg
      541 agacgatgaa gttcgaggac gcagccggaa tactcggtgc gagctgtgcc aaggagatcg
      601 atacaaactg ccgcggcgtc agtctcgatc ctgctcgtct gaaggattgc ctcttgcgca
      661 atcaggatag tttatcggct cagtgcagga ctgattatgt tcgagctttc gacgcgatcc
      721 agaaacgggt ggccgcccgc tccgctgtcg ccaaaatatg tgagcgcgac gccgtcaaat
      781 tatgcggcgg cattcaaaag gatggcgtcc aggctctcga atgcatcttg acggcgccgc
      841 gcgggttaag tgccagatgc aatcaggcca ttggtgaggc ggggtatcgc tgatgcgatt
      901 ggggaagaaa agccggcatg gcattgggtt gttcatcgtc ctgctgattg gctccatggc
      961 gccgctgtcc tcggctcggg cccaggcgag tgccccgacg gaagactggg tcaaacagct
     1021 tgctggcctt gaacctgcgc ccgatatgga gatcgcggcc ctgcggcaac aggcgctgga
     1081 gagagttaag tccaaggcgg atggcgtgcc gctgcagcga ccgcctgtcg ccgcgcagct
     1141 gctcaagctg ccaagacttg ccgtcgagat tcaattcgat gaggactctt cgatcattcg
     1201 gccagagtcc tatcgaacgc tcgggcgcat cgccgatacg ctttatgatc ccgcgctgct
     1261 gggttacaaa ttcctgattg tcggccatac cgtggcgacg ggtaagcgcg aatacaatct
     1321 gactttaagc cagaaaagag ccgacgcgat ccgcggggcg ctcgtgaccg ccttcaagat
     1381 ttcgccaaaa cgcctgcagg ggatcggtct gggcgaggag caactgctgg atgccgccca
     1441 tccgaccgcg ccgatcaacc agcacgttga agtcgcgacg ctgggcaaag ctttttagca
     1501 acgcggctac tgcttttgaa ttccggcctt ctcgatcacc ttctgccact tgtcgatgtc
     1561 ggatttgagc cgggctgaaa tttcctgcgg cgtactcgct ctggcctcga tgccaagttc
     1621 gatcagccgc tttttgacgt cggcgtcggc gagtatatcc tgcaacgcgc cattgagctt
     1681 gttgacgatc tcgggcggcg tgccggtggg cgcaaacaac gcgttccagg agacgacgtc
     1741 gtaattggca acaccgcttt cttgcacggt ggcaacggcc ggcgtgcttt tcgagcgcac
     1801 gggccccgat gacgccagcg cccggaactt cctgtcgtca agattgccct tcatcgaaga
     1861 gtagctgtcg atcacaagcg ctatgtcgcc ctgcaacagc gagatctgaa cctcgggtgt
     1921 tccacgatgg gggatgatcg tgaaatcgat gtcagctgat gtcttgaaca attccgccga
     1981 caggttctgg gtgctgccga tattgatggt gccgacattc agcgcgccgg gttttgcctt
     2041 tgcggcggcg atgaaatccg caagcgtcct gagctccgaa ttggcgccgg tggcaaagac
     2101 gaaatcgaaa aaacccagac tggaaatcgg ttcgaaatcc cgcaagggat cgaaaggcag
     2161 tttcttgaac agggagacgc tgaccgcggt gccattggac agcaacgcca gcgtatagcc
     2221 gtccggcgcc gatgcgatca cggttcgggc tgcggcgatg ccgccggcgc cggcctggtt
     2281 ctcgacgtag aaccgttgtc caagcttgtc gccgagtttt tccgcaacaa tgcgcgccgt
     2341 ggtgtcggcg acgccgccgg ccgcgaatgg cagaacgatg cgcacggact tgttcggata
     2401 gctcgcctcc gcgcgcgcct catggtgtga agctgcgagc gcggatagcg caaacacagc
     2461 cggaaatatc gcgaggcgta ggcgggagac ggcgcgacca actgttgtca tggacgactt
     2521 tttggtttcc tggacgaggt tgaagtgacc gatatttatg tcgcggtcaa tcgcgaccag
     2581 gtgcggcgag ccgataaagg ttccacccat aatggatttg ttttctccct aaccgaagct
     2641 tgcggtcatg ggctcagtaa acaaacttgc gcaccgcgca gcacgagcaa tgcctttcac
     2701 ctgtcggctg ccggaaccat tgacccgcga gcctgttagc ctgctgacac aataactttt
     2761 ggagaaatgc catgaatatc ctgtcgatct ctctggcagt tggagcaagc gcgtttttca
     2821 tgactgctgc ttccgccgca ccgctttccg ccggtgcatc gatcgcaccg gagagcaaca
     2881 ttcaaaacgt ccgcatggtc tgcaatgaaa acggtcgttg ctggcgtgaa cgcagtgagc
     2941 gccgcgtcat catccgtgag ccccgtgatt cttacggata cgcccctcgt gagggttatg
     3001 tcgagcgccg gggttacgac gaggaccgcg gcataggttt tagagctccc ggtgtgagca
     3061 ttggtatcgg cacagagcgc tactgatcgc tccgggtacc cggccaccac cagcatgtga
     3121 cgtgaaagat tgcgcccggc cgacttcgtt tggccggggc gcatgattga ggcgctatca
     3181 agaagcgaac tccaattcta attttgcaaa ttctaatttt gcaaattcta attttgaatg
     3241 tgagcactat tgagcagtaa gtcggaacct aagaggccat gatggttcca tcactggctc
     3301 atcctcggca tccaccggtg acagagagtc ttgtgctccc gcccgtttga gcgaagcatc
     3361 atgccggcat ccgaatcgca gcattccggc agttcgccgg ttgcaatcga aactcccgca
     3421 tgtccaaagt gccagggttc gatgatgctt tcgcggataa ggccgggacg cctgaacttc
     3481 gacgcacgca cgtttgagtg cgtcaagtgc agtcatgttg aaaggatcct cgtcgcgagc
     3541 gaccccatgc agtccgatgt gcgtggatgg ctgttgggcg agctcaaggc gccgatctag
     3601 atccgctagc gcttcccgat tttctcccac agccagcgcg ccaccgcatc gggcgacgag
     3661 acgccgtcgc cgccggcggc acgcaagttt gcctcccgca tcgtggcaat atcgatttgg
     3721 ccgagcagcg gcgagagcgc cgcgcgcaat gcctgatccg acgcgcgttt cggcgccagc
     3781 agcacgatcg cgtcgtatgg cgggatggcg tgtttggcat cgtcgagcac gaccagatcg
     3841 tatttcgcaa tcagcccgtc gctggtgtag cccgcgatca cttcgacctc gccggaggcg
     3901 acagcggcat acatgaagtc gggctgcatc tgccgttgag cgcgaagcga cagcccgtag
     3961 gcctctcgca acccggccca ttccggccgc gaaaagaact cgtagtcggc ggccatcgac
     4021 atcgctggcg catgcgaggc gagatcggcg atggaatgga tccccagttc ctcggcgcgt
     4081 tttcggggca tcaccagcgc gtaggcgttc tcgaacccga gttccccgag caatgtgatt
     4141 ttttgcgtcg ccagcgtcgc tttcaattcg tcgagcaatt cttcgcgcgg cttgatatcg
     4201 ctgtggtgaa actggttggc ccacagcgtg ccggaataat cgacatagac gtcgatatca
     4261 cctgacgcca gcgcatcgaa gatcacgttg gagccgaggc cctcgcgcga gcttgcggac
     4321 aattccgccg cccgcagccg ctgcgcgatc agcgccgaca gcacatattg ttcggcaaag
     4381 gtttttgcgc cgacgatata agtcgattgc gagcgcgtca ccgcgggcac cagggttgcc
     4441 gcgaccagca ccgcgatgcc gacgccgccg aacgccaccc ccaggcggct gcgctggcga
     4501 aggccggtct cgatcagggc cagcaattga tcgaccgcga gcgccagcac cgcggcggcg
     4561 aggcagccga acagcacgaa cacccagttc tgagtctgca gccccgcaaa gatgtagttg
     4621 ccgaggctgg tctgtccgat cggggtcgac agcgtcgcgg tgccgatcac ccacaccgcc
     4681 gcggtgcgga tgcccgccat catcaccggc agcgccagcg gcaactccac tgacaatagc
     4741 gactgacgct ccgtcatgcc gacgccctgc gcggcctcga ggatcgcagg ctcgacgccc
     4801 tgcaggccgg tgatggtgtt gcgcagcacc ggcagcatgg aataaagcgc cagcgccagc
     4861 accgcgggaa ggaatccaaa tgcggaaaag ccaaccccga accaggcaag cgacagttgg
     4921 gcgagcgcca gcagcagcgg atagaacagc gcgagcagcg cgaggcccgg caccgtctgc
     4981 acaatgctgg cgaggcccag tagtgcgccg cgcatcaccg gccggttgcg cgagaaaatc
     5041 gccagcggca ggctgaccag aaggcccagc gccagcgccg tgacgctgac acgcacgtga
     5101 ttgccgagat aatccggcag atgtccgagc gcctcgctcc agcgcggatc ggagaacaga
     5161 ctcatgccgc gccaccccgc ggcagcagca cgttgagccg tt
//
LOCUS       DASWGM010000175        20395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_15721,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000175 DASWGM010000000
VERSION     DASWGM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20395
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15721"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VGA15_05325"
     CDS             complement(<1..440)
                     /locus_tag="VGA15_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9587148.1"
                     /translation="MRVINVAAAQMGPIQRADSREVVVKRMLALMDEAKAGGADLIVY
                     PELALTTFFPRWYMEDQAEVDTWFEREMPNAAAMPLFARAAEYQMAMSFGYAELTHDG
                     HHFNTCILTDKSAKIVGKYRKVHLPGHSEFDTERAFQHLEKRYF"
     gene            1115..2365
                     /locus_tag="VGA15_05330"
     CDS             1115..2365
                     /locus_tag="VGA15_05330"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX9587149.1"
                     /translation="MTGSNRVDHIRITSHPAPGAKHDYPIDWGAASARERGPVIGTVS
                     RPQDRNVIGTHGGSYSVYRALAVSSGALDPIRRPDLTNTHPAETIGPFSQWADPQRIV
                     SLDPWGHLVAENFAKEIAEGIDVRPSIAVTRARLDLPELQAAIEAGRLKHDGEVVHKN
                     GSVSVVKIAIDPVWYLPGMAARFATTENNLRRQLFEQTAGMFPELVTRPDLRVFLPPI
                     GGTTAYLFGDVTKLPDHRTRITCRVHDECNGSDVFGSDICTCRPYLLHGIEECARAGQ
                     TGGLGIIIYNRKEGRALGEVTKFLVYNARKRQEGGDAAAQYFERTECVAGVQDARFQQ
                     LMPDVVHWLGLKRIDRFVSMSDMKHDALTGQGIEIVERVPIPDDLVPADAHVEIAAKK
                     AAGYYTSEPQQDPTDTVGRSLEKY"
     gene            2383..3621
                     /locus_tag="VGA15_05335"
     CDS             2383..3621
                     /locus_tag="VGA15_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="URC4/urg3 family protein"
                     /protein_id="HEX9587150.1"
                     /translation="MVNLDTSAALSLLNTRAVRERAHRILAIGLEDKLPNFRIHLDRM
                     DSTVDLVLRMTREAYPSFDVPFHSRWRHFVTNGDNRWATIADQTRWPDGAARARAEFD
                     LAIVSVFLDAGAGPSWRYHDPKTGSAIGRSEGLGLASLAMFAGGAFSADPREPLRADA
                     DVLAHFSVADLERGMQVSDVNPLLGIDGRADLLRRLGRLVASKPDLFGSHDTPRPGGL
                     FDRLAMLADDRQLPAPTILSELLRTLGPIWPSRLTLGGIALGDCWKHPMLTTVDATSG
                     LMPLHKLSQWLAYSLIEPLQTAGISVTDIDGLTGLAEYRNGGLFVDNGVLAFRDAEDA
                     QQEHEVASSLVVEWRALTVALLDGVADGLRHRLKLDASSLPLAKILEGGTWAAGRLLA
                     RERRADVSPPVKVISDGTVF"
     gene            3638..4267
                     /gene="upp"
                     /locus_tag="VGA15_05340"
     CDS             3638..4267
                     /gene="upp"
                     /locus_tag="VGA15_05340"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX9587151.1"
                     /translation="MDGVTIVSHPLVQHKLTLLRKKEISTKSFRELLKEIGMLLCYEI
                     TRDLPLADIDIETPLAHMKSPQIAGKKLVFAPVLRAGVTFAEGMLDLVPTARIAHIGL
                     YREPETFVAVEYFFKAPSDLHERLVIVISPVLATANSAVAAVDRLKERGANDIRLVCL
                     IGAPEGVERMRGIHPDVPIWLAAIDKGLDENAYIFPGLGDAGDRAYGTK"
     gene            complement(4405..6147)
                     /locus_tag="VGA15_05345"
     CDS             complement(4405..6147)
                     /locus_tag="VGA15_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9587152.1"
                     /translation="MSRVHKVATVQFEPTMFEKERNIARLLELCEAAALAGARLIVTP
                     EMGTTGYCWFNRAEATPFVEKIPGPTTDRFVALARKHDCHIVVGMPEVDGDGIYFNCA
                     ALIGPDGIVGRHRKTHPYISEPKWAAPGDLGHQVFDTPIGRIALLICMDIHFIETARL
                     AALRGADIICHISNWLAERTPAPYWISRAYENGCYVIESNRWGLERTVQFSGGSCVIA
                     PDGSVTAVLDSGDGFVVADIDLDTARSRLVGGEAVFAQRRPELYAELLSNTFSWNPRD
                     FFGLYGHQPWPDGRQSRISVAQFMPGNKVAANLDRIADLAREAKADGAELVVFPELAA
                     TGLSDSAASAELIPGPTTDRLCALAAQLDLYLVCGLAERSGEAVYNSACLVVPDGTVA
                     VYRKIHLTSAERSWAMAGDQWTVVDTPIGRIGLMIGHDASFPEAGRILALRGCDIIAC
                     PAAIKQRFSTPHTGSQVAQRFPIPTGADPHHWHHFRVRAGENNVFFAFANILDPENGY
                     PGLSGVFGPDTFEFPRREAIVADVEGVTTGVVDTTNLDTNYPTNVVRRKDLVSMRMPH
                     SYRELIRTERVSGD"
     gene            complement(6163..7152)
                     /locus_tag="VGA15_05350"
     CDS             complement(6163..7152)
                     /locus_tag="VGA15_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX9587153.1"
                     /translation="MAWQHNSIMASKGIAKGKPGIHHMITEADQGKYHYVYGPYVEPV
                     LSVDPGALVSAETHDAFEGAIKYETDSPSKILNFPFLNPQNGPIWINGAEKGDTLAVY
                     IQSIVPRGPQPRGTTVIMPEFGGLVGTGQTALLNPPLPERVKKLEIDLNTGTKWNDKI
                     TLPYEPFIGTIGTSPEIEAISSLQPDYYGGNMDLPDVGVGAVVYLPVNTKGGLLYLGD
                     CHAAQGDGELCGVAIEHPTVTTLQVDLIKGWTIAWPRLETEQFIMTIGSARPMEDAAR
                     IAYRELCRWMAADYGFEEIDAYMLLTQAGRVRLGNMVDPKYTLGASIKKSFLR"
     gene            complement(7194..7907)
                     /locus_tag="VGA15_05355"
     CDS             complement(7194..7907)
                     /locus_tag="VGA15_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587154.1"
                     /translation="MRKEVILSTLGLRAGYGGKPVLQGLEIEVREGEIVAVIGRNGVG
                     KSTLMKSLIGLVPAMSGSIVYRGQAIELLPAHKRARLGVGYVPQGRDVFPRLTVAENI
                     AVGAAVRGDIPEEGRQRIVEAFPILAERWSQRAGTMSGGQQQQLAIGRVLIADPNLIL
                     LDEPSEGIQPNIVQDIARNMVELNRKTGVTIILVEQNLDMIRAMAQRCYVMDKGRFVA
                     TLDRAGLDDEAEMRRHLAV"
     gene            complement(7900..8616)
                     /locus_tag="VGA15_05360"
     CDS             complement(7900..8616)
                     /locus_tag="VGA15_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587155.1"
                     /translation="MALLELRRLNKHFGGLHVTNSVDLALETGEIHCLIGPNGAGKST
                     LFRLILGEHHPGSGDIFFAGENITALKSFARIHRGLSVKFQVPGVFKGLSVRQNLEIA
                     LQRQHRAGALAREIDRLLTFLGLEAEQTQSAGNLSHGQKQWLEIGMAISLKPRLLLLD
                     EPTAGMSPEETHLTGEMVQRLNAEGMTVLAIEHDMAFVRQVAQRVTVLHLGQVFAQGS
                     IEEIVADERVAAIYLGQAHA"
     gene            complement(8616..9680)
                     /locus_tag="VGA15_05365"
     CDS             complement(8616..9680)
                     /locus_tag="VGA15_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587156.1"
                     /translation="MNGFLVFLRRLEGPQTVGRGPAFWALFAIVLAFACAYPLFSDGY
                     TVGNTVYFFVWVFIALSLCVIWGYGGSLSFGQTAFFGIAGYGYGILTINFGSAHGFTL
                     IALLLAIGLAALFALLLGYFMFFGRIAGVFLGIVTLAVTLMLERFMAQTAGPEWHIGV
                     ARLNGFNGMSAMPPLTIPWPSGDIVLFADIGLYYAVLGLLVLVYLGLRILMNSAFGNV
                     IVAIRENPERAEMLGYDVRKYQLITFVIGAALAGLSGVLYTVWGQYITPSSMGMTAAA
                     LPLIWVAVGGRSDLTSTLIGTLVVLAAFQALTIYGSQYALVFMGVLLVLTVLIAPNGL
                     VLAVMNFISRLAAGRSKGDA"
     gene            complement(9693..10565)
                     /locus_tag="VGA15_05370"
     CDS             complement(9693..10565)
                     /locus_tag="VGA15_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587157.1"
                     /translation="MDIATLAFSALYQFGDAFAFLVLSACGLAVIFGMMGVINLAHGE
                     FIMCGAYVTASTAHAGLPLPLAIMAGAIVAALVGALVELAVIRHLYDRPLDTIVATWG
                     LSLIATQGTLIVVGSTMAGIGTPFGSFQVGQYSYSIYRLVLFASALGVLGGLYMLFNW
                     TRFGVLARATIQVPHMAAALGVDTRLIYSLTFACGAGLAGLAGGLYAPTMTLVPTMGA
                     TFIMEAFVTVVIGGADVFLGTAPAGGVLAVVKSAMTSWQGQLFGQIGLLVAVIIVVRI
                     LPKGISGFLLRERT"
     gene            complement(10682..11920)
                     /locus_tag="VGA15_05375"
     CDS             complement(10682..11920)
                     /locus_tag="VGA15_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEX9587158.1"
                     /translation="MTKNVLRGLRAVALTGTLVLSSGAAFAENSIKLGVLEDQSGDFA
                     AATIGKVHAIQLAADEINKAGGIAGRPLELVTYDTQSDNTRYQEFMRRVLQRDKVDVV
                     FAGFSSASREAYRPIVDQFNGFAFYNNQYEGGVCDGHMVVTGAVPEQQFSTLIPYMME
                     KYGKKVYTLAADYNFGQISAEWVRKIVKENGGEMAGEEFIPLGVSQFSQSIQNIQKAK
                     PDFVVTLLVGTAQASYYEQAASANVNLPMASSVNVGQGYEHKRFKPPSLKDMYVTTNY
                     IEEIDSPASKAFYAKFKAKFPSEPYVNQEAENSYLAIYLYKQMVERAKSTNREDIRKV
                     IALGDVCMDAPEGKVCIDPKSQHMSHTIYLAKVGADHSISFPKVWEDIKPYWLGEAGC
                     DLTKKDPMAQYTPSNPPPKP"
     gene            complement(11932..12597)
                     /locus_tag="VGA15_05380"
     CDS             complement(11932..12597)
                     /locus_tag="VGA15_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX9587159.1"
                     /translation="MTSRLLQNFKGGRALMVTGRCGWEGTLETTLAKLGVTSEYPRIV
                     GGRAQIDVGSLQPERDILFVDGDLEGAVAIEVNPASRLPPVPVIGLVGVEAPSRLKAL
                     VNLGATSFLRKPVYGGAVYASLFMGINQFLLRSDMYARLQDLEGRRRGRRAVIHAVIL
                     LMQQDGLDEEGAYSQLRRGSMRARQNLELYCEEFLSRRAKSPDIPRRATSMTLQGGNK
                     QAM"
     gene            complement(12594..13754)
                     /locus_tag="VGA15_05385"
     CDS             complement(12594..13754)
                     /locus_tag="VGA15_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9587160.1"
                     /translation="MARKQCRIGVMFSTTGSYSVVARSMLNGALLAFREIAEAGGAVT
                     LEPVVVNPSGDLARYRALSLELLGAGIRQVVGCYTSSSRKEVIPCFEKFDGLLWYPSH
                     YEGFESSDNVIYTGASPNQHVLPLVDYLASRVGARAFCVGSNYIWAWENNRIFREALT
                     ARGGTVVAERYLSVGDTEFDQVIAAILDQRPDFVFNNLIGTSAYAFFRAFRAACRARG
                     IDQASDIPVASCTLSEPELSEIGEDAVDGHLSSSVYFSSLDSSANATFIRAYAKAFPD
                     GPVSSADAEASYIAVKLLAAALEQAGTDDARTVRAAVANQRLQAPQGEVRIDPQTYHA
                     WLTPRIGRSAVNGQFEVLLEARNPVAPDPYLVQSSPRFAVAMRSPVLRVVQS"
     gene            15154..15843
                     /locus_tag="VGA15_05390"
     CDS             15154..15843
                     /locus_tag="VGA15_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9587161.1"
                     /translation="MFGPSSEFAQASDDAKSALIATSTRRAYADNEVIYLQEERAQNL
                     YFVVSGHVRLSYVLDDGSAILYAILPAGDSFGELGIFDGGVHGDMAMGIGNTVIGAVS
                     AKNFHALCHRFPALHDCIAQLVARRYRSYIELTRIMSLKTLPGRLAQALLRLADGLGT
                     ATNYCGRDVPCVGPVITQADLGLMARGARGSVNRVLKTWQRAGWLVVKDRAILIADRA
                     ALEAIAIEGDV"
     gene            16235..16927
                     /locus_tag="VGA15_05395"
     CDS             16235..16927
                     /locus_tag="VGA15_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587162.1"
                     /translation="MSMPLDNNSHLSATASYRNSDDLEPVRQPAWRSVHAIDEAAEEV
                     RAIVATYLTPTDSTRALQALNHVYVGAPQNIKDAIMHNPDALLILECAAEEANAPLTK
                     KVAVPVLPKERTHAAILCLQQATRRLDARLAGAVVGLAVPGHEAFCERNAGILPGDGL
                     FGPAGADLLRDVIDRIAGTPEGNDVAARFMAMGALYYGVDKDVGERHSSNDLSTTPMR
                     ELPGATQTRRRD"
     gene            complement(17098..17235)
                     /locus_tag="VGA15_05400"
     CDS             complement(17098..17235)
                     /locus_tag="VGA15_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587163.1"
                     /translation="MKRPRAYRIAGFCELERAIARPRSRPEFAEDLRAAQPVLLILHF
                     S"
     gene            complement(17538..17666)
                     /locus_tag="VGA15_05405"
     CDS             complement(17538..17666)
                     /locus_tag="VGA15_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587164.1"
                     /translation="MSTLRKSCRSADTGPSLGKPALSESMAGILSAITAEWQFNPP"
     gene            complement(18118..18327)
                     /locus_tag="VGA15_05410"
     CDS             complement(18118..18327)
                     /locus_tag="VGA15_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587165.1"
                     /translation="MRDTEWIWQVADENEVGEVEARQHRRGCASAGFDDRAGANVASF
                     GAVPEVARPDWSRIGTARRGPRVAF"
     gene            complement(18907..19044)
                     /locus_tag="VGA15_05415"
     CDS             complement(18907..19044)
                     /locus_tag="VGA15_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587166.1"
                     /translation="MITRRTFTTGAATMLAAGHLSTRGFVLLLTLFPGIVTILPRMLY
                     G"
     gene            complement(19096..>20395)
                     /locus_tag="VGA15_05420"
     CDS             complement(19096..>20395)
                     /locus_tag="VGA15_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX9587167.1"
                     /translation="VALFSCTSHLMDIGGIGFGPDATDVFMEGLYIPMLKLIDQGVVN
                     ETLMAMIRSNTRLPIDTEGDTYSLAGCNDVGCQRLVEMMREFGIESLDQLGDHICDRS
                     RGAVLAEIARLPKGVWRNSMVVDGYDEPVTLVATLTISDDGIHVDYVGTSAASKFGIN
                     VPLSYTTAYTVFGLGCVVSAQIPNNAGSLLPLTVAAPAGSILNAPKPAPVASRHVIGQ
                     MLPDVVFGCLSQIIPERVPAEGTSCLWNLNVRGQTRSGAGGNYGFSMAVTSNGGTGAR
                     FAKDGLSATAYPSGVRGTPVEIAETQTPLIFWRKELRPDSGGPGRARGGLGQIIEVGS
                     GVDAPFDILAAFDRIDHPPRGRDGGRDGEAGYVGLKSGQKLRGKGFQTVPPDDRLVVL
                     TPGGAGIGDPRERAPTSVRDDVESGLVSSENAVAVYGFDG"
BASE COUNT         4020 a         6313 c         6246 g         3816 t
ORIGIN      
        1 tcgaaataac gtttttcaag gtgctggaag gcgcgctcgg tatcgaattc cgaatggccc
       61 gggagatgaa ccttgcgata cttgccaacg attttcgctg acttgtcggt gaggatgcag
      121 gtattgaaat gatgtccgtc atgcgtcagc tcggcatagc cgaagctcat cgccatctga
      181 tattccgcag cacgcgcgaa cagcggcatt gccgctgcgt tcggcatctc gcgctcaaac
      241 caggtatcga cttcggcttg atcttccata taccagcgcg gaaaaaatgt ggtgagtgcg
      301 agctccggat aaacgatcag gtctgctccg ccggccttgg cctcgtccat cagagcgagc
      361 atgcgcttaa cgacgacttc ccggctatcg gcgcgctgaa tcggccccat ctgagcggcg
      421 gcgacattga tgactcgcat agtgaacttc ctggctgctt taatttcacg ctgcattgga
      481 caaaaaaggg gcagttcgag cgttgcgcct ttcgcagtcc atttccgtaa ttgatttcac
      541 tattagaatt ctgattgccg cgaagtcaat tcagttcagc gaaggtctgt aaaacggttc
      601 tgcatatcat gctggccgag atggcgtggc gatagtctaa aacgctgacg accagagcga
      661 cattacccga aggcttgcgt tttcatccgc gaactcgaat atcgacctta agttgcgagg
      721 ttggtgagcg aagctcgcat ttcaatcgat ggcatcattg ccgcgatggt tatcggcttg
      781 cgcaagtttg attgcccccg tagatagatt tttaatcgcg tcctgccgtt tacgtggacc
      841 ctgtaagttc ggcactttat tcaggtgacg ggcttcctga ataaggctat tcttcgctac
      901 gcctactgat atcgccggag cacggcacga acgggcgcgg cgtccacgct gaagaagtta
      961 gcagccaagg cgtccccggg attcgaatcg ccgttgtgtt cggtcgcgat ttgattatac
     1021 ttccgtgttc ggctgcggcc gcgctactaa tattatcgtt ggtgaccagg cccgtctgct
     1081 taggcgggcc gtcgccattt aagggtccat ggcgatgact ggttccaatc gggttgacca
     1141 cattcgtatt acctcgcacc cggcaccggg tgccaagcac gactacccga tcgactgggg
     1201 cgcggccagc gcgcgcgaac gcggaccggt gattggcacg gtctcgcgcc cacaggatcg
     1261 caacgtcatc ggtacccatg gcggctccta ttcggtttat cgcgcgctcg ccgtctcatc
     1321 gggcgcactc gatccgatcc ggcgcccgga cctcaccaac acccatcccg ccgagacgat
     1381 cgggccgttt tcgcaatggg ccgatccgca gcggatcgtt tcgctcgatc cgtggggcca
     1441 tctcgtcgcg gaaaacttcg cgaaagagat cgccgaaggc atcgatgtcc gtccgagtat
     1501 tgccgtcact cgcgcgcgtc ttgatctgcc cgaactgcag gcggcgatcg aagccggccg
     1561 gctcaagcat gacggcgagg tcgtgcacaa gaacggaagc gtctccgtgg tcaagatcgc
     1621 gatcgacccg gtatggtatc tgccgggcat ggcggcgcgc ttcgcgacca ccgagaataa
     1681 tctgcgccga caactgttcg aacagaccgc gggcatgttt ccggagctgg tgacacggcc
     1741 cgacttgcgg gtttttctgc cgccgatcgg cggcacgaca gcttatttgt tcggcgacgt
     1801 taccaaactg ccggaccacc gcaccaggat aacctgtcgc gtgcatgacg aatgcaacgg
     1861 ctccgatgtg tttggctcgg acatctgcac ttgccgtcca tacctgctcc acggcatcga
     1921 ggaatgcgcg cgcgccgggc aaaccggcgg actaggcatc atcatctata atcgcaagga
     1981 aggccgcgcg ctcggcgagg tcacaaaatt tcttgtctac aacgcgcgca agcgtcagga
     2041 aggcggcgac gccgcggccc agtatttcga gcgcaccgaa tgtgtcgccg gggtgcagga
     2101 tgcccgattc cagcaattga tgccggatgt ggtacactgg ctcggcctga agcgaatcga
     2161 ccgcttcgtt tctatgagcg atatgaagca cgatgcgctg accggccagg gcatcgaaat
     2221 cgtcgaacgt gtaccgattc ccgacgatct tgtgcccgcc gacgcgcatg tggaaattgc
     2281 ggcaaagaaa gccgccggtt actacacgtc cgagccgcag caagacccga ccgacacggt
     2341 cggccgttcg ctcgaaaaat attgagaggg acgccagtgt ccctggtaaa tctcgatacg
     2401 tcagccgctt tgtcattgtt gaataccagg gcggttcgcg agcgcgcgca tcgtattctc
     2461 gcgatcggcc ttgaagacaa gcttccgaac ttccgcatcc acctcgatcg catggactcg
     2521 acagtcgatc tggtgctgag gatgacgcgc gaggcttatc cctcgttcga cgtgcccttt
     2581 cattcgcgct ggcgacattt cgtcacgaat ggggacaatc gctgggcgac catagccgac
     2641 cagaccaggt ggcccgatgg cgctgcccgc gcccgggctg aattcgatct ggcaatcgtc
     2701 agtgtcttcc tcgacgccgg cgctggcccg tcgtggcgct accacgatcc aaaaaccgga
     2761 tcagctattg gccgttcgga gggattagga ctggccagcc tcgcaatgtt cgctggcggc
     2821 gcattctcgg ctgatccgcg tgagccgttg cgggccgatg ccgacgtact tgcacatttc
     2881 tccgtcgcgg acctcgagcg cggcatgcag gtctcagatg tcaatccgct gctcggcatt
     2941 gacggtcgcg ctgacctgtt gcgccgcctc ggccggcttg tggcctcgaa gccggaccta
     3001 ttcggcagcc acgacacgcc gcggcccggc ggcctgttcg atcgacttgc gatgctggca
     3061 gacgacagac agcttcccgc cccaaccatt ctatcggagc tgctgcgaac actcggaccg
     3121 atctggccat cgcggctgac gctgggcggg atcgcgctcg gcgactgttg gaagcatccg
     3181 atgctgacga cggtggatgc gaccagcggt ttgatgccgc tgcacaagct ctcgcaatgg
     3241 ctggcctatt ccctcatcga gccgctacaa acagctggca tcagcgtaac cgatatcgat
     3301 ggcctcacgg gccttgcaga atatcgcaac ggcgggctgt tcgtcgataa cggcgtgctc
     3361 gcatttcgcg atgcagaaga tgcgcaacaa gagcatgagg tagcgtcatc gctggtcgtg
     3421 gaatggcgcg cgctgacggt ggcgctgctc gacggcgtcg ccgatggctt gcggcaccga
     3481 cttaagctcg atgccagctc gctgccgttg gcgaaaattc tcgagggtgg aacatgggcc
     3541 gcgggtcgat tgctggcgcg cgaacgccgt gcggacgttt cgcctccggt gaaagttatc
     3601 agcgacggca ctgtcttcta gatcacggat cagcaacatg gacggcgtca cgatcgttag
     3661 ccacccgctg gtgcagcaca agctgacgct attgcggaaa aaagaaattt cgaccaagtc
     3721 gtttcgcgag ctcctcaagg agatcgggat gctgctgtgt tacgaaatca cgcgcgatct
     3781 accgctggcc gatatcgata tcgagacgcc gcttgcgcat atgaagtcgc cgcagatcgc
     3841 aggcaagaag ctggtgttcg cgccggtgct gcgggccggc gtgacctttg cggaaggcat
     3901 gctcgatctg gtgccgaccg cacggatcgc ccatatcggc ctttaccgcg aaccggaaac
     3961 cttcgttgcc gtagaatatt tcttcaaggc gccaagcgat ctgcacgagc ggctggtgat
     4021 tgtgatctcg ccggtgctgg caacggcgaa ctccgccgtc gcggcggtcg accgactgaa
     4081 ggagcgcggc gcgaacgaca ttcggctggt gtgccttatc ggcgcgccgg aaggtgtcga
     4141 gcgcatgcgt ggcattcacc cagacgttcc gatctggctc gccgcgatcg acaaagggct
     4201 ggacgaaaac gcctacattt ttccgggtct tggcgacgcc ggcgaccgtg cctacggcac
     4261 caaataggct agctgggtgc gcagtgtcgt ggaatcagcc gccactgttc gaaatctgcc
     4321 catacgcagc gatagggggc gcggctggtg gccgggagcg ttgccgcctt tcccttacct
     4381 gccgaacatg cgatcctccc tcgctcaatc gcctgaaacc cgctcggtcc tgatcagctc
     4441 acgatagcta tgcggcattc gcatggagac gagatccttc cgccgaacaa cattggttgg
     4501 gtaattcgta tcaagattgg tggtgtcgac cacccctgtc gtgacgcctt cgacatcagc
     4561 cacaatcgct tcacgacgcg gaaattcaaa cgtatccggg ccgaacacgc cgcttagccc
     4621 gggatagccg ttctccggat caaggatgtt ggcaaaggcg aagaagacgt tgttctcgcc
     4681 ggcacgaacg cggaaatgat gccagtggtg cggatcggcg ccggtcggaa tcgggaagcg
     4741 ctgcgccacc tgtgatcccg tgtgcggcgt gctgaagcgc tgcttgatcg ccgccggaca
     4801 ggcgatgatg tcgcagccgc gtagcgcaag gatacggccg gcctctggaa acgacgcgtc
     4861 atgaccgatc atcagtccga tgcgcccgat cggcgtgtcg acgaccgtcc attggtcgcc
     4921 ggccatagcc cagctccgct ccgccgaggt cagatggatc ttgcgataga ccgcaactgt
     4981 tccgtccgga acaaccaggc aggcgctgtt gtagacggcc tcgccggacc gctcggccag
     5041 cccgcagacg agataaagat caagctgcgc agccagcgcg caaagccggt ccgtcgtcgg
     5101 tccggggatg agctccgccg atgcggcgga atcgctgagg ccggtcgccg ccagttccgg
     5161 aaacacgaca agctcggcgc cgtcagcttt ggcctcgcgc gcgagatccg cgatccggtc
     5221 gagattcgcc gcgaccttat ttccaggcat gaactgcgcg acgctgatcc gcgattgccg
     5281 tccatccggc cagggttgat gtccatagag cccgaagaaa tcgcgcggat tccagctgaa
     5341 agtgttgctc agaagctccg catagagctc cggccggcgc tgcgcaaaga cggcttcgcc
     5401 gccaacgagg cgcgaccgcg cggtatcgag atcgatatcc gcgacgacaa aaccgtcgcc
     5461 gctgtcgagc accgccgtca cgctcccatc cggcgcaatc acgcaactgc cgccgctgaa
     5521 ctgaacggtg cgttcgagcc cccagcgatt gctctcgatg acatagcaac cattctcgta
     5581 ggcgcggctg atccagtatg gcgcgggcgt acgctcggcc agccagttcg agatgtggca
     5641 gatgatgtcg gcgccgcgta gcgcggcgag ccgcgcggtc tcgataaaat ggatatccat
     5701 gcagatcagg agtgcaatgc gcccgatcgg cgtatcgaac acctgatggc cgagatcgcc
     5761 cggcgccgcc catttcggtt ccgaaatgta gggatgcgtt ttgcggtgcc ggccgacgat
     5821 gccgtcaggt ccgatcaggg cggcacagtt gaaatagatg ccgtcgccgt cgacctccgg
     5881 catgccgacg acgatatggc aatcatgttt ccgggcgagc gccacaaagc gatcggtcgt
     5941 cggccccggg atcttctcga caaagggcgt cgcctccgcc cggttgaacc agcaatagcc
     6001 tgtcgtgccc atctccggcg tgacgatgag acgggcgccg gcaagtgccg ccgcctcgca
     6061 gagttcgagc agccgcgcaa tgttgcgctc cttttcgaac atggtcggtt cgaactgaac
     6121 ggtggcgacc ttgtgcacgc gcgacatcat cgatctgccc ctttagcgga gaaacgattt
     6181 cttgatcgag gcaccgaggg tgtatttggg gtccaccatg ttgccgagcc ggacgcgccc
     6241 ggcctgggtc agcagcatat aggcatcgat ctcctcgaag ccgtagtcgg ccgccatcca
     6301 gcggcatagt tcgcgataag cgatacgcgc ggcgtcttcc atcggccgtg ccgagccgat
     6361 cgtcatgatg aactgctcgg tttcgagccg cggccatgcg atagtccagc ccttgatcag
     6421 gtcgacctgg agcgtggtca cggtcggatg ctcgatagcg acgccgcaga gttcgccgtc
     6481 gccttgcgcg gcatggcaat cgccgagata gagcagcccg cccttggtat tgacaggcag
     6541 atagaccacc gcaccgacgc cgacgtcggg caggtccatg ttgccgccgt aataatccgg
     6601 ctgcagcgac gagatcgcct cgatctccgg cgacgtgccg atggtgccga tgaacggctc
     6661 atagggcagc gtgatcttgt cgttccattt ggtgccggtg ttgagatcga tctcgagttt
     6721 tttgacgcgc tcgggcaatg gcggattcag gagcgcggtc tggccggtgc cgaccaggcc
     6781 gccgaattcc ggcatgatga cagtggtgcc gcgtggctgc ggcccgcgcg gcacgatgct
     6841 ctggatatag accgcaagcg tatcgccctt ttcggctccg ttgatccaga tcggcccgtt
     6901 ctgcggattg agaaaaggaa agttgagaat tttggacggg ctgtcggtct cgtacttgat
     6961 cgccccctcg aatgcgtcat gggtttcggc ggagaccagc gcgcccggat cgaccgacag
     7021 caccggctcg acgtaggggc cgtagacata gtggtacttg ccctgatccg cctcggtgat
     7081 catgtgatgg atgccgggtt tgcccttggc gatgcctttg cttgccatga tcgaattgtg
     7141 ctgccaggcc atcgatgttc tccttctctg ttgttgttcg gcgtgagatg gcgtcagacc
     7201 gcgaggtggc ggcgcatctc cgcttcgtcg tcgagccccg cacggtcgag ggttgcgacg
     7261 aagcgaccct tgtccattac gtagcagcgc tgcgccatgg cgcggatcat gtcgagattc
     7321 tgctcgacca ggatgatcgt cacgccggtc ttacggttga gctcgaccat attgcgggcg
     7381 atgtcctgga cgatattagg ctgaatgcct tcggacggct cgtcgagcaa aatcaggttg
     7441 gggtcggcga tcagcacacg cccgatcgcg agttgctgct gctggccgcc ggacatggtg
     7501 ccggcgcgct ggctccagcg ctccgcgagg atcggaaaag cttccacgat ccgttgccgt
     7561 ccttcctcgg ggatgtcgcc cctgacggca gcgccgacgg cgatgttctc ggccaccgtc
     7621 aggcgcggaa acacgtcgcg cccctgcgga acgtagccca cgccaaggcg cgcccgcttg
     7681 tgcgccggca acagttcgat ggcctggcca cgatagacga tcgacccgct catcgccggc
     7741 accaatccga tcaggctctt catcaatgtc gacttgccga cgccgttgcg gccgatcacg
     7801 gcgacgatct cgccttcccg cacctcgatt tcgaggccct gcagcaccgg cttgccgccg
     7861 tagccggcgc gcaggccaag cgtcgagagg atcacttcct tacgcatgcg cctgccccag
     7921 atagatcgcg gcgacgcgct cgtcggcgac gatctcctcg atcgagcctt gcgcaaacac
     7981 ctggccgaga tgcagcaccg tgacccgctg cgcgacctgg cgcacgaagg ccatgtcgtg
     8041 ctcgatcgcg agcacggtca tgccttccgc attcagccgc tgcaccatct cgccggtcaa
     8101 gtgcgtttct tccggcgaca tacccgcggt cggctcgtcg agcagcagca ggcgcggttt
     8161 caggctgatt gccatgccga tttcgagcca ctgcttctgg ccatgactga ggttgccggc
     8221 actctgcgtc tgctcggctt cgaggccgag gaaggtcagc agccggtcga tctcgcgcgc
     8281 aagcgcgcca gcgcggtgct gccgctgcaa ggcgatttcg aggttctggc gaaccgacag
     8341 ccccttgaac acgccaggca cctggaactt gaccgacagc ccgcggtgga tgcgcgcgaa
     8401 cgatttcagc gcggtgatgt tctcgccggc gaagaagata tcgccgctgc ccgggtggtg
     8461 ctcgccgagg atcagcctga acagcgtact cttgccggcg ccgttgggac cgatcaggca
     8521 atggatttcg ccggtttcga gcgcaaggtc gaccgagttg gtgacgtgca ggccgccgaa
     8581 atgcttgttg agcctacgaa gttcaaggag cgccatcagg catctccctt gctgcggccc
     8641 gcggcgagcc gggagatgaa attcatgacg gcgagaacga gcccgttcgg cgcgatcagg
     8701 acggtcagca ccagcagcac gcccatgaac accagcgcgt actggctgcc gtagatggtc
     8761 agggcctgga aggccgcgag caccaccagg gttccgatca gcgtcgaagt cagatcggaa
     8821 cgcccgccga ccgcaaccca gatcagcggc agcgcggccg ccgtcatgcc catgctcgac
     8881 ggcgtgatgt attgccccca taccgtgtag agcacgccgg agaggccggc gagcgccgcg
     8941 ccgatcacga aagtgatgag ctgatacttg cgcacgtcgt agcccagcat ctctgcgcgc
     9001 tccgggtttt cccgaatcgc aacgatcaca ttgccgaatg cggaattcat gaggatgcgc
     9061 aggccgagat agaccagcac cagaagaccg agcacggcgt aatagaggcc tatgtccgcg
     9121 aacagcacga tgtcgccgct cggccagggg atggtcagcg gcggcatggc gctcatgccg
     9181 ttgaaaccgt tcagacgcgc gacgccgatg tgccactcgg gacccgcggt ctgggccatg
     9241 aagcgctcca gcatcagcgt caccgccagc gtcacgatgc cgaggaagac gccggcgatg
     9301 cgaccgaaga acatgaagta gccgagcagc aaagcgaaca gcgcggcaag gccgatcgcc
     9361 agcagcaggg cgatcagggt gaagccatgg gctgaaccga agttgatggt gaggatgccg
     9421 tagccgtaac cggcaatgcc gaagaacgcg gtctggccga agctgagcga gccgccatag
     9481 ccccaaatca cgcataggct gagcgcgatg aacacccaga cgaagaagta gacggtgttg
     9541 ccaaccgtgt agccatcgct gaacagtgga taggcacagg cgaacgcaag cacgatggcg
     9601 aacagcgccc agaaagccgg accgcggccc acggtctgcg gtccttcgag gcggcggagg
     9661 aaaacaagaa agccgttcac tgccgcgatc cttcaggtgc gttcgcgcag caggaagccg
     9721 gagataccct tcggcagaat ccgcacgacg atgatcacgg caaccagcag accgatctgg
     9781 ccgaatagct gcccctgcca ggatgtcatc gccgacttca ccacggcgag cacgccaccg
     9841 gccggcgccg tgccgaggaa tacgtcggcg ccgccgatca ctaccgtgac gaaggcttcc
     9901 atgatgaagg tggcgcccat ggtcggcacc agtgtcatgg taggggcgta caagccgccg
     9961 gcgaggccgg cgaggccggc gccgcaggca aaggtgaggc tgtagatcag gcgcgtatcg
    10021 acgccgagcg ccgcggccat gtgcggcacc tggatcgtgg cgcgcgcgag gacgccgaaa
    10081 cgggtccaat tgaacagcat gtagaggccg ccaagaactc ccagcgcgga tgcaaacagc
    10141 acaaggcggt aaatggaata ggagtactgg ccaacttgaa agctgccgaa cggcgtgccg
    10201 atacctgcca tcgtggagcc caccacgatc agcgttcctt gggtcgcaat caggctcagg
    10261 ccccaggtcg ccacgatcgt gtcaagcggc cggtcgtaga ggtgtcggat caccgcaagc
    10321 tcaaccagcg cgccgaccag cgcggcgacg atagcgccgg ccatgatcgc cagcggaagc
    10381 ggcaggccgg catgggccgt cgatgccgtc acataggctc cgcacatgat gaattcaccg
    10441 tgggcgagat tgatcacgcc catcatgcca aagatcaccg cgagcccaca tgccgacagc
    10501 acaaggaacg cgaacgcgtc gccgaattga tagagcgcgg agaatgcgag ggtcgcgatg
    10561 tccatgaact aaccggatgt tgcaaattcg aaatgcgatg ccgtgccgtc gcgagcttca
    10621 atggttcaag gccgcagccc cggcgcgcag cagcattcgc cggggcgacg ccgacgcctt
    10681 gtcagggttt cggcggcggg ttcgatggcg tgtactgcgc catcggatct ttcttggtca
    10741 ggtcgcagcc cgcttcgccg agccagtacg gcttgatgtc ctcccatact ttcggaaagg
    10801 agatcgagtg atcagccccg accttggcga gataaatggt gtgcgacata tgctgactct
    10861 tcgggtcgat gcagaccttg ccctccgggg cgtccatgca gacgtcgccg agcgcgatca
    10921 ccttgcggat atcttcccga tttgtcgact tcgcccgttc gaccatctgc ttgtagaggt
    10981 agatcgcgag ataggagttc tcggcctcct ggttcacgta gggttcgctg ggaaacttgg
    11041 ccttgaactt cgcgtagaaa gccttgctgg ccggcgagtc gatctcctcg atgtagttgg
    11101 tggtcacgta catgtccttc aggctcggcg gcttgaaacg cttgtgctcg tagccctgcc
    11161 cgacattgac cgacgaggcc atcggcaggt tgacgttggc ggaagccgcc tgctcgtagt
    11221 aggaggcctg cgcggtgccg accaggagcg tcaccacgaa atccggcttg gccttctgga
    11281 tgttctggat gctctgcgag aactgcgaca cgccaagcgg gatgaactcc tcgcccgcca
    11341 tttcgccgcc gttctccttg acgattttgc gaacccattc cgccgagatc tggccgaaat
    11401 tgtaatcggc ggcgagcgta tagaccttct tgccgtactt ctccatcatg tagggaatga
    11461 gcgtggagaa ctgctgctcc ggcactgcgc cggtgaccac catgtgtccg tcgcagacgc
    11521 cgccctcgta ctggttgtta tagaacgcga agccgttgaa ctgatcgacg atcggtcgat
    11581 aggcctcgcg cgacgcggat gagaagccgg cgaacaccac gtcgaccttg tcacgctgca
    11641 gcacgcgccg catgaactcc tgataacgcg tgttgtcgga ctgagtgtcg taggtgacga
    11701 gttcgagcgg ccggcccgcg atcccgccag ccttgttgat ctcgtcggcg gcgagctgga
    11761 tcgcatggac cttgccgatg gtggccgccg cgaaatcgcc ggattgatcc tccagcacgc
    11821 cgagcttgat ggaattttcc gcgaaagccg cgccagacga gaggacaagc gtccccgtga
    11881 gggctacggc gcgcagcccc cgcaacacat tcttggtcat gtcggtgtct cctacatcgc
    11941 ttgcttgttg ccgccttgca gcgtcatgct ggtcgcgcgg cggggaatgt cgggcgattt
    12001 cgcccgcctg ctcagaaact cctcgcagta gagttccaga ttctgccgcg cgcgcatgct
    12061 cccgcggcgg agctgggaat aggcgccctc ttcatcgaga ccatcctgct gcatcagcag
    12121 gatgaccgcg tgtatcaccg cgcgtcggcc gcggcggcgc ccttcgagat cctgaagacg
    12181 ggcatacatg tcgctgcgca gcaggaactg gttgatgccc atgaacagcg aggcgtaaac
    12241 agctccgcca tataccggct tgcgcaggaa ggaggtggcg ccgagattga ccagcgcctt
    12301 gagccggctc ggcgcctcga cgccgaccaa gccgatcacc ggcaccggcg gcaggcgcga
    12361 ggcgggattg acctcgatcg caaccgcacc ttcgaggtcg ccgtcgacga acaggatgtc
    12421 gcgttcgggc tgcaggctgc cgacgtcgat ctgcgcgcgg ccgccgacga tccgaggata
    12481 ttcgctggtg acgccgagct tggcgagcgt ggtctcgagc gtgccttccc atccgcagcg
    12541 ccccgtgacc atcagggcgc ggccaccttt gaaattctgc agcagtcgag acgtcatgat
    12601 tgcaccactc tgagcacggg ggagcgcatt gcgaccgcga agcgcggcga cgactggacg
    12661 agatagggat ccggggcgac ggggttgcgc gcttccagca gcacctcgaa ttgtccattg
    12721 acggccgagc ggccgatccg gggcgtcagc caggcgtgat aggtttgcgg gtcaatccgc
    12781 acttcaccct ggggcgcctg caggcgctgg ttcgcgaccg cggcgcgcac ggtgcgtgca
    12841 tcatcggtgc cggcctgctc cagcgccgca gccagaagtt tgaccgcgat gtatgacgct
    12901 tccgcatcgg cagacgacac tggcccgtcg ggaaaggcct tggcatacgc ccgaatgaag
    12961 gtcgcgttcg ccgacgagtc cagtgaggag aaatacacgc tggaactgag atgcccgtca
    13021 acggcatcct cgccgatttc cgaaagctcc ggttccgaca atgtgcagct cgccaccgga
    13081 atatcgctgg cctgatcgat gccgcgggcg cggcaggcgg cgcggaaggc gcggaagaag
    13141 gcgtaggcgc tggtgccgat caggttgttg aagacgaaat caggacgctg atcgagaatc
    13201 gcggcaatca cctggtcgaa ttcggtgtca cccacggaca gataccgctc cgcgaccacg
    13261 gtgccgccgc gcgcggtcag cgcctcgcga aagatacggt tgttctccca ggcccagatg
    13321 tagttggagc caacgcagaa cgcgcgggca ccgactcgcg aggcgagata gtcgaccagc
    13381 ggtagcacgt gctgattggg cgaggcgccg gtgtagatca cattgtccga gctttcgaag
    13441 ccctcgtaat gcgacggata ccagagcagc ccgtcgaact tctcgaagca cggaatgacc
    13501 tccttccggc tcgaggacgt atagcagccg accacctggc ggatgccggc gccgagcagt
    13561 tcgagactga gcgcgcggta gcgtgcgaga tcgcccgatg gattgaccac gaccggttcg
    13621 agcgtcaccg ccccgccggc ttccgcgatt tcgcgaaagg cgagcagcgc gccgttcagc
    13681 atcgagcgcg cgacgacgct ataagacccg gtcgtcgaga acatcacgcc gatacgacac
    13741 tgcttccgcg ccatccgcct caatccaaaa caaaaaacgc ccaccggctg tgaagccgag
    13801 ggcgccctcg ccaccaaccg gacacgcttg aatttcagcg tggtgttgga aatggtttga
    13861 acttgtcgcg ttaatgggca gctttgccca acgctcgtag cttaggctcc cttagatcga
    13921 atgtgatgtc aagcgtgttg ttagagcggt aattgcttca attcatatct tgctgcagca
    13981 aaactatgca gcggtcggaa agaaacaaac ctactcatca gggtcggaat cgccgatgag
    14041 aaaccgcaac gcagcccgtg cacacaccga caccggcggt gcgatgcggg tgatttttgg
    14101 agctggcaaa tatcgctggc tgaactttgc aaggtcacgc ataggctcga catcaaccag
    14161 gcctctatcc ccgatgacga cgacgctggg cggcgtgcaa aagagaagcg cctcggttac
    14221 gttgcgatga tcggatttgc cggacagttt gccggacagt cggcttcaag gccatttttt
    14281 cgtcaacttc gcgattatcg ccgcgcgcgc tgatcatgct gatgcgcgtc gcagcagctc
    14341 cgctgcggag aagcctcgaa ctgttcagag gcgtttcttg tttccaattg gccctgctgc
    14401 aagggaatgg acgaactgga gaatttcacg cgtgtgacct tccgcatatt ggcttatccg
    14461 ggatcatgcc cgccccgatt ggaaatcgaa tcgtcacatc tgcattctca gtacagatcc
    14521 ggccacggac accaaatgca cacgcgccgc ggccttccca attgcgctcc gacgaaaacg
    14581 ctgagcacag gcacattcaa gtgaaatcag cattggcaat gaagcccgca ttgatcaagc
    14641 gcattgcgga caaatcatac gcgccgcaac gtcgacatag ttcgagaaac aaccagccat
    14701 cggcgccttg acgtccgaac ttcccagatc gtgggggagc cccgaacacc agcctggact
    14761 ccattgagta tcttccgaat gacgcgttcg aatgaccgag actcacccat ggatagggct
    14821 gatactcgat aggcacagtt gtagatctcg gaagtaggct ccaggcttca tacgtgctgt
    14881 tcgaacacga gggtgagtga cggcgcgaca cttgtgcgaa agcgacatag acttggacca
    14941 gaacgaagtc gggaaacgtt gcgcatccgc gatgccggat ggcgacaccg cgatcggcac
    15001 gacgatgcgg cgcatggcta gatgcctctg acatcgacgc tggatttgat cgccggtttg
    15061 ccgtaacccc atcatcattg atagtcaggg ttatgcgctc gttgaaatcg catccggctg
    15121 ttcgcctaac caaggcccat cacggaattc cagatgttcg gaccttcgtc ggaatttgcg
    15181 caagcgagcg acgatgcaaa aagtgcgctc atcgcgacct ccacccgtcg ggcctatgcc
    15241 gacaacgaag tcatctatct gcaggaagag cgcgcgcaaa acctgtattt tgtcgtttcc
    15301 ggccatgtgc gcctgtccta tgttttggat gacggttccg cgatcctcta tgccatcctg
    15361 ccggcaggcg atagtttcgg cgaactcggc atatttgacg gcggcgtaca tggcgacatg
    15421 gcgatgggaa ttggcaacac cgtaatcggc gccgtctcag caaagaactt tcatgcgctc
    15481 tgtcaccgct tcccggccct gcacgattgc attgcccagc tggtcgcgcg gcgttaccga
    15541 tcctacatcg agttaacccg cattatgagc ctcaagacgc ttcccgggcg cctggcgcag
    15601 gcgctgttgc ggctcgccga cggattgggg accgcgacaa attattgcgg acgcgatgtg
    15661 ccgtgcgtcg ggccggtgat tacgcaagct gatttggggc tgatggccag aggcgcacgc
    15721 ggcagcgtca atcgggtgct gaaaacctgg cagcgcgccg ggtggctcgt cgtgaaggac
    15781 cgcgcgattt tgatcgccga ccgcgccgcg ctcgaggcaa tcgctatcga gggagatgtc
    15841 tgaaatgttc aatgctcacg cgaggaagtc cgctttcctt gcaatgacgg aaacgcgcaa
    15901 tgtccgacat ccgtcatcgc ctggcgttcc ctaccattga ccctgcgcga ggctcacagc
    15961 atgagatgat cgttgacggc atcggcgtgc cctgcccgat gcagaggcct cgcattcgcg
    16021 aggaattcga cctttcccgc tcccgctcag cccggcggcc tttgttttcc ccaccagggc
    16081 tggctcattc gtccggcccc gcactgttgc acgatccacg cattccattg aattcggtgc
    16141 ctgggtgtaa tttacggcac actccgccgg agaaatccgt agtctaagtc cgcaccggtg
    16201 cggttaccca cgattttgac ggaagacacc ctccatgagt atgcccctcg ataataactc
    16261 tcatctcagt gcgacggcga gctaccggaa ttcagatgac ttagagccag ttcgacaacc
    16321 agcctggcgg agcgtccacg caatcgatga agccgctgaa gaggtgcggg ccattgttgc
    16381 aacgtatcta acgccgaccg acagcacgag ggctctgcaa gcgctcaacc atgtttatgt
    16441 aggcgcgccc cagaacatca aagatgcgat aatgcacaac cccgacgctc tgctgatcct
    16501 ggagtgtgct gccgaggaag caaacgcacc gctgacaaaa aaagtcgccg ttccggtatt
    16561 gccaaaagaa cggacccacg ccgccatcct ttgcctccaa caagcaacga gacgtctcga
    16621 cgcgagattg gcgggcgcgg tggtgggcct ggccgtgccg ggccatgagg cgttctgcga
    16681 gcgcaatgcg ggtatcctgc cgggtgatgg actattcggg ccagctggcg cggatctgct
    16741 tcgtgatgtg atagatcgca tcgcgggcac ccccgagggc aacgatgtgg ccgcgcgctt
    16801 tatggccatg ggcgcattgt actacggcgt agacaaagac gttggcgaaa gacactccag
    16861 caacgatctc tcgacgacac caatgcgcga actgcccggg gcgacccaga cgcgcaggcg
    16921 agattgagcg atggatcgca ccagcagaag gtggagggtg caactgcaaa actcaagttg
    16981 gcttttgccg ccgaaatggc caccagggtc ggcatgacga ccaaggtcgg gcgttgacac
    17041 tggtgacgca taggcgaagc gagcgggcca gaatttagcg gaaaatatcc ttgccactca
    17101 gctaaaatgg aggatcagca gcaccggctg cgcggcgcgc aaatcctccg cgaattccgg
    17161 gcgactgcgc ggccttgcga ttgcccgttc aagttcgcag aaacctgcga tgcgataggc
    17221 gcgagggcgc ttcatcggca atttcagaca ttcgttcgcc agagtgaatg cctcgtcgac
    17281 ttgccgagac gcgcgccaca aagcgccagc cctgataggg ctttcgcctt ttccatcttg
    17341 agatgcagca gggtcgcaaa gagcaccgcc gcccggctta tatatccggc tctaagaccg
    17401 tccgcggcgc aatcatgaac gaggggcgca atctgtttcg cttcgaaggc cccgctgaag
    17461 cgtcgtccga agattgcagc ctggataccg gcgatgcctt catcagccca ttcggtcgcc
    17521 cctggcgtca ggactgatta cggcggattg aattgccatt ccgccgtgat agccgacagg
    17581 atgcctgcca tggattccga cagcgcgggt tttcccagtg acgggccggt atccgccgag
    17641 cggcaggatt ttcgcaatgt gctcatgcag tttcgcaacc acatcgcaat ttttcttcgg
    17701 cactcaagtc cgagcattgg cttggcttcg cgcatcagcc gactttcgat acttcaaaaa
    17761 ccgacgaaga cctaagcagc acgtctcgcc aacaggttga aggcggcgcc gcaccgcggc
    17821 gcttatcgcg tcgcttcact cgtcttcgcg aagaaacttg atgccgcgca gccgccgtga
    17881 cagcgctggg gcgaccgcga ggcccgcgac caccgagacg acggcccacg tggcaaggat
    17941 cttgagaacg atcattgcgc cccctcggca aatttcgaac gaaactccgg tgcgatcgcg
    18001 aagccacgga acccccacgc gcggtcgcca gcgccaggcc gacgtgacgc gccgcggctg
    18061 cggtgcgcca acgtcatgct ccggtaagca cgcgtgctcg cgctctcagt acagccttca
    18121 gaacgcgacg cgcgggccgc gtctggcggt gccgatacgg ctccagtcgg gccgcgcgac
    18181 ctcagggacg gcgccgaacg acgcgacatt tgcgccggcg cggtcgtcga agccagcgct
    18241 cgcgcaaccg cgccgatgct gccgcgcctc gacttcgccg acttcgtttt catctgcaac
    18301 ctgccagatc cattcggtgt ctctcaatgg atatagcgct cctcgagaca aaaaagccgc
    18361 cgcacgcgcg aaacctagat gaggcagaaa cgaaagactt gggcggatac gacggcggct
    18421 tgtccaaaac cgcaaaatca cgatttccct cagccgtggg agcgaatttg cgacgcgaag
    18481 tgccccgggc agcgaagttc ttcgtcgcag cgctatcgtc aaataaatcg agacatggac
    18541 ggtttcaacg ctaccgggca actggcagca gcttcgtgat cactgggcag cgtttcatgt
    18601 catccgagtg gttgcgtccc ctgcgggact tctccttctg gcagccggag caatctactg
    18661 ttagatgaac acctgctgcg ccaaggtcgc tcccccgagt tagcagactt gatagttgtt
    18721 ccgcaaacaa ctggaggaca ctactaagag cctttgccat tccccgcgct tttgtttgcc
    18781 caaccctctt aggcatgcgg ctcgggatct tcaggtcagg tcaaaaagca cgcttgaacc
    18841 gtgttctgca taaagcgtat ccgtgggcat gattcgccgc ggcagtttcg acagtatacg
    18901 ggcggtttat ccgtagagca ttctgggcag gatggtgacg atgcctggaa acagcgtcag
    18961 cagcagcacg aagccgcgcg tcgaaagatg gccggcggcg agcatcgtcg ccgctcccgt
    19021 cgtgaaagtc cgccgcgtaa tcatgcaccc tctccggttt gttgaaattg aaacgcaaag
    19081 ccgccgccgg acccatcagc cgtcgaagcc gtaaaccgca accgcgttct cgctcgacac
    19141 caggccactt tcgacgtcgt cgcgcacgct cgtcggcgcg cgctcccttg gatcgccgat
    19201 cccggcgccc cctggcgtca gcaccaccag ccgatcgtcc ggcggcacgg tctgaaaacc
    19261 cttgccgcgc agcttctgtc ccgacttcag tccgacatag ccagcttcgc cgtcacggcc
    19321 gccgtcacgg ccacggggcg gatgatcgat gcggtcgaag gccgccagaa tgtcgaacgg
    19381 cgcatcgacg ccgctgccga cttcgatgat ctggccgaga ccaccgcgcg cgcggccggg
    19441 gccgcccgaa tccggacgca gctccttgcg ccagaagatc aacggcgtct gggtctccgc
    19501 gatctcgacc ggcgtgccgc gcacgccgct gggataggcc gtggccgaca acccatcctt
    19561 ggcaaaacgc gcgcccgtgc cgccgttgct cgtcaccgcc atggagaatc cgtaattgcc
    19621 gccggcgccg ctgcgcgtct gtccgcgcac attgagattc cacaggcagg acgtgccttc
    19681 ggccggcacc cgctcgggaa tgatctgact gaggcagccg aagacgacgt cgggcagcat
    19741 ctggccgatg acatgacgcg acgccaccgg cgccggcttc ggcgcgttga ggatcgagcc
    19801 cgcgggagcc gccacggtca gcggcagcag cgagcccgcg ttgttcggga tttgcgctga
    19861 caccacgcag ccgaggccga acacagtgta agccgtggtg taagacagcg gcacattgat
    19921 gccgaacttc gaagccgccg atgtgccaac ataatcgacg tgaatgccgt cgtccgagat
    19981 cgtcagcgtc gccacgagcg tcacaggctc gtcatagccg tcgacgacca tgctgttgcg
    20041 ccaaaccccc ttcggcagcc tggcgatctc cgccaggacc gctccacgcg agcggtcgca
    20101 aatgtgatcc ccaagctgat cgagcgactc gatgccgaat tcgcgcatca tctcgacaag
    20161 acgctggcat ccgacatcgt tgcagcccgc cagcgaatag gtgtcgcctt cggtgtcgat
    20221 cggcaaccgc gtattgctgc ggatcatcgc catcagcgtt tcgttgacga cgccctgatc
    20281 gatcagcttc agcatgggga tatagagccc ctccatgaag acgtcggtgg cgtccggccc
    20341 gaagccgatg ccgccgatat ccatcagatg gctggtgcag gaaaacagcg cgacc
//
LOCUS       DASWGM010000176         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_429278,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000176 DASWGM010000000
VERSION     DASWGM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429278"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VGA15_05425"
     CDS             <1..564
                     /locus_tag="VGA15_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9587168.1"
                     /translation="VYDEFVGRVAEYGKKLQVGNGLDPNTQIGPLVSEQQLDRVTGYL
                     DVGRQEGAKALAGGARLTEGALSKGYFVPPTVFANVQDNMRIAQEEIFGPVISAISFK
                     DGDELIQRANATTFGLGSGVWTRDVSKAHRVAKALRAGSVWVNCYQAMDPAVPFGGYK
                     MSGYGRESGKQHLEEYLNVKAVWIKTG"
     gene            complement(630..1013)
                     /locus_tag="VGA15_05430"
     CDS             complement(630..1013)
                     /locus_tag="VGA15_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9587169.1"
                     /translation="MSKQCVKSVRSLPSLSVRIDLDSEGRIGPGKIQLLENIHSCGSI
                     SAAGRAMDMSYKRAWDLVDEINRICRHPAVERQTGGKNGGGAVLTPFGMSLVARYRKI
                     ERDAASAVRKELLALRTDIGRPKKA"
     gene            1121..2248
                     /locus_tag="VGA15_05435"
     CDS             1121..2248
                     /locus_tag="VGA15_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX9587170.1"
                     /translation="MNYPSLFSPLQIGPYQLRHRLVMAPLTRMRAEKPSLAPRPLNAE
                     YYAQRATPGGLIIAEASPVVATGFGSPGVPGIYSEHQIKGWREVVDAVHAKSGVIFLQ
                     LWHVGRVSHSSFQPGGVLPVAPSAVPISADLKTMTADGKVVSYETPRALDTSEIPGVI
                     EAYRQAARNALKAGFDGVEIHGANGYLIEQFLQSHTNLRTDQYGGSIANRARFLMEVT
                     QAVIDVWGADRVGVRLSPYGVANDSGEADPMPLYTHVIKSLDPLGLAYLHFVEPRSSG
                     AGRAEVNHQNVPSAAVLFRPIWSGKLITAGGFTGETANAAISAGHADAIAFGRIFISN
                     PDLPRRLQRGFPLTPYNRATFYGGEESGYTDYPVHDELEQA"
     gene            2353..2856
                     /locus_tag="VGA15_05440"
     CDS             2353..2856
                     /locus_tag="VGA15_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9587171.1"
                     /translation="MTTKPKAVGVSAGQCLCGSVCFEIDTPARWAWHDHSLGSRRAHG
                     AAYATYVGSWRKRFRITKGKAGIARFEDKLTKTVRSFCARCGTPLFYERARSPHMVNI
                     PRALFTGRTGRQPLYHIAIEELQEWAYTGEPLVPLKGFPGVVWQRSKRNKRIAGDDPF
                     ELSRADS"
     gene            3070..>4050
                     /locus_tag="VGA15_05445"
     CDS             3070..>4050
                     /locus_tag="VGA15_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9587172.1"
                     /translation="MNRITGRSAFLALLKDEGITHLFGNPGTTELPIMHALKDHPDLT
                     YVMAMQESLVVAIADGFSRASGRLVACNVHVAPGLGNAMGSLYNASFTGTPMILTAGQ
                     QEQGHGLTEPLLYGPLVRMAEPLVKWAVEVTRLEDLPRIVRRAAKIATTPPTGPVFIS
                     LPGDILNAEAGIELGRSTRVDTRVKPSDEVLQALVARILKAERPVIIAGDEIVKSNAL
                     HEAAALAMTLGCPAYQQSAPYGAHFLSESPCFMGALSRAQPQVREVLSPYDLMIVLGA
                     DPLRMSVHSEVDPLPEGLAILQIGLVEWDLAKNYGAEIALKADVKETLRVL"
BASE COUNT          733 a         1266 c         1278 g          773 t
ORIGIN      
        1 gtgtacgacg aattcgtcgg ccgcgtcgcc gaatacggca agaagctgca ggtcggcaac
       61 ggcctcgacc cgaacacgca gatcgggccg ctggtctccg agcagcaact ggatcgcgtc
      121 accggctatc tcgatgtcgg ccgccaggag ggtgccaagg cgctggccgg cggcgcccgg
      181 ctgaccgaag gtgcgctgtc gaagggctac ttcgtgccgc cgaccgtgtt cgccaacgtc
      241 caggacaaca tgcggatcgc tcaggaggaa atttttgggc cggtgatctc ggctatctcg
      301 tttaaagatg gcgatgagct gatccagcgc gccaatgcca cgacgttcgg cctgggcagc
      361 ggcgtctgga ccagggacgt cagcaaggcg catcgcgtgg cgaaagcgct tcgcgccggt
      421 tcggtgtggg tgaattgcta tcaggcgatg gacccagcgg tgccgttcgg cggctacaag
      481 atgagcggct atggccgcga gtcaggcaag cagcacctgg aagagtatct caacgtcaag
      541 gcggtctgga tcaagacggg ctaggcgttt tggatttgcc atatccggga gtggcggttt
      601 cgactgccac tcccgttttg cttgccggct caagccttct tcggccgtcc aatgtccgtc
      661 cgcaacgcca gaagctcctt gcggactgcg ctcgccgcgt cgcgttcgat tttacgataa
      721 cgcgctacca aggacatgcc gaacggcgtc aggaccgcgc cgccgccgtt cttgccgccg
      781 gtttgccgtt cgacggcggg atgccggcag atacgattga tctcgtccac cagatcccac
      841 gcccgcttgt aggacatgtc catcgcgcgg ccggcggccg agatcgagcc gcagctgtgg
      901 atgttttcaa gcaactggat tttgcccggc ccgatgcgtc cctccgagtc aaggtcgata
      961 cgaacactca gcgatggcag cgacctgacg cttttgacgc attgttttga catggaagat
     1021 ctgctctgcc gctccgggcc ttcagattgc catcgaaggc ttttgcaaac aagacgaacc
     1081 ggttacatat cctgatcaac acacattttc cggggcgtac atgaactatc cttcgttgtt
     1141 ttctccgctg cagatcggtc cgtaccagct cagacatcgc cttgtgatgg cgcctctgac
     1201 gcgaatgcgg gcggaaaagc ccagcctcgc gccccggccg ctcaacgcgg aatattatgc
     1261 gcagcgcgcg acgccgggag ggttgatcat cgcggaagcc tcgccggtcg tggcgaccgg
     1321 ttttggaagc cctggcgttc ccggcatcta ttcggagcac cagatcaagg gctggcgcga
     1381 ggtcgtcgat gccgttcacg ccaagagcgg agtcattttt ctccagctct ggcatgtcgg
     1441 acgggtttcg cactcctcgt tccaacccgg cggcgtgttg ccggtcgcgc cctcggcggt
     1501 gccgatttcg gctgacttga agaccatgac ggctgacgga aaggtcgtgt cctatgagac
     1561 gccgcgtgcg ctcgacacca gcgagattcc cggcgtgatc gaggcctatc ggcaggccgc
     1621 gcgcaacgcg ctcaaggccg gcttcgacgg cgtcgagatc catggcgcca acggctatct
     1681 gatcgagcag ttcctgcaat cccacaccaa tctgaggacc gaccagtatg gcggatcgat
     1741 cgccaaccgc gcgcgattcc taatggaggt cacgcaagcc gtgatcgacg tctggggcgc
     1801 cgaccgcgtc ggcgtgcggc tgtcgcccta tggcgtggcc aatgactctg gcgaagccga
     1861 tccgatgccg ctttatacgc atgtgatcaa atcgctcgat ccgctcggct tggcctatct
     1921 gcattttgtc gagccgcgat cgagcggtgc cggccgtgcc gaggtcaatc atcagaacgt
     1981 gccgtcggcg gccgtgctgt ttcggccgat ctggagcggc aagttgatca cagccggcgg
     2041 ttttaccggc gagaccgcga atgcggcgat ttcagccggc catgccgacg cgatcgcatt
     2101 cgggcggatc ttcatttcca atcccgacct gccgcgccgg ctgcagcggg gtttcccgtt
     2161 gacgccctac aacagggcga ctttttacgg cggcgaggaa tccggctata cggattatcc
     2221 tgttcacgat gagctggagc aggcttgata acccaaacgt cattgcgagc gcagcgaagc
     2281 aatccatcgc caagtcaatg gattgcttcg tcgcttcgct cctcgcaatg acgtggagcg
     2341 aggagagcgc cggtgacgac gaaaccaaaa gcggttggag tctccgcagg ccaatgcctg
     2401 tgcggtagcg tctgcttcga gattgacacg ccggcgcgct gggcctggca cgatcattcg
     2461 cttggcagcc ggcgcgcgca tggcgcggct tacgcgacct atgtcggaag ctggcgcaag
     2521 cgttttcgca tcaccaaggg caaggccggg atcgcgcgct tcgaggacaa actgaccaaa
     2581 accgtgcgca gcttctgtgc gcgatgcggc acgccactgt tttacgaacg ggcgcgttcg
     2641 ccgcacatgg tgaacattcc gcgcgcgctg ttcaccggcc gcactggccg gcagccgctc
     2701 taccatatcg caatcgagga gctgcaggag tgggcttata ccggcgagcc gctggtgccg
     2761 ctgaagggtt ttccaggcgt ggtgtggcag cgctcgaaaa ggaacaagcg catcgccgga
     2821 gacgatccat tcgagctcag tcgcgcagat tcatgaaagc ctgatatatt cttgtgcctt
     2881 gcaccgcgtt tcggctcagg catttgccgg gaacactatg aatcgcaggc atgcaacaca
     2941 agagctacgg cgctcgcgcg cgcccgcgca gggcagccat gatcgccgtc cattgatctc
     3001 ccccttcaag atcggccatg ttgtcaccga cgcccgcggt aatggccgcg gcggaggagg
     3061 agatctttca tgaatcgcat caccggccgt tcggcgttcc tcgcactgct caaggacgag
     3121 ggcatcacgc atctgtttgg caatcccggc accaccgaac tgccgatcat gcatgccctg
     3181 aaagaccatc ccgacctcac ttatgtgatg gcgatgcagg agagcctggt ggtcgcgatc
     3241 gccgacggtt tcagccgcgc ctcgggccgc ctcgtcgcct gcaatgtcca tgtcgcgccc
     3301 gggctcggca acgcgatggg atcgctttac aacgccagtt ttaccggcac gccgatgatc
     3361 ctgaccgccg gccagcagga acaaggccac ggcctgacgg agccgctgct gtatgggccg
     3421 ctggtccgga tggccgagcc gctggtaaaa tgggcggtcg aggtgacgcg gctcgaggac
     3481 ctgccacgga tcgtgcgccg cgcggccaag atcgcgacca cgccgccgac cggcccggtg
     3541 ttcatctcgc tgcccggcga tatcctcaat gcggaagccg gcattgaact gggccgttcg
     3601 acccgggtcg atacccgcgt aaaaccctcc gacgaggtct tgcaggcgct ggtagcgcgc
     3661 atcctcaagg ccgagcgtcc ggtcatcatc gccggtgatg aaatcgtcaa aagcaacgcg
     3721 ctgcatgaag ccgcggcgct ggccatgacg ctcggctgtc ccgcctatca gcaatcggcg
     3781 ccctacggcg cgcattttct ctcggaaagc ccgtgcttca tgggcgcgct gtcgcgcgcg
     3841 cagccacagg ttcgcgaggt gttgtcgcct tacgatctga tgatcgtgct cggcgcggat
     3901 ccgttgcgga tgtcggtgca cagcgaggtc gatccgttgc ctgagggcct ggcgatcctg
     3961 cagatcggcc tggtcgagtg ggacctcgcc aagaactacg gcgccgaaat cgcgctcaag
     4021 gccgatgtga aggaaaccct gcgggtgctg
//
LOCUS       DASWGM010000177         6000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_149956,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000177 DASWGM010000000
VERSION     DASWGM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149956"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VGA15_05450"
     CDS             complement(<1..443)
                     /locus_tag="VGA15_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9587173.1"
                     /translation="MSGLPHSPHALVTGGGRGIGRAIASSLAQAGATVTVLGRHRAAL
                     DDAVAAGAAHFAGVADVADQAAVSAAVAEAAGRQPIDILIANAGIAESAPFGKSDAAL
                     FRRMMDVNFMGVVHAVQAVLPAMKDRRYGRIVAIVSTAGLKGYAYV"
     gene            588..1037
                     /locus_tag="VGA15_05455"
     CDS             588..1037
                     /locus_tag="VGA15_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9587174.1"
                     /translation="MAALEKGITRNGTGYSGKTWNILGQVYFPKAVTDSTFAFETNSD
                     PGQFVPVHIHPTQEEFILVQDGMLDLKLDGVWVQANAGDLVRLPRGISHGYFNKSDKP
                     ARALFWVSPTQKLEALFNQLHNLTDVAEIVRISAEHEVNFLPPEAND"
     gene            1148..2644
                     /locus_tag="VGA15_05460"
     CDS             1148..2644
                     /locus_tag="VGA15_05460"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9587175.1"
                     /translation="MVKQKNVCIIGAGVAGLAAAKIFDARGHKVIILERSGDLGGVWE
                     PARSYPEVQTQSPKDLYRYTDKPMPNSYPEWPTGPQVHAYLTDYAGEHGLMHRMRLNT
                     TVVGMARRNADLPGWTLEIRNPYGGVTHEEFDFVAVCTGQFNEPQVISHPGEDAFKAA
                     GGRILHSAQHTDSSFVKGKKVVILGGSKSATDIAVNSVNAGASEVTLVYREPVWRIPY
                     FIGGLLNFKRILYTRAQEEMFRSWGIGGISRAAHAIGKPLVWANWRALESLLKLQLKL
                     KKCGMVPKTRIEDGVNCSVPIATPEFFPMVADGRIKAIRGTFDRYDGKTIIMTGGERV
                     AVDIAILAIGFKLGVPFLPEAYRNKLVEPDGQYRLYRLIANPDLPDMGFVGFNSSFCT
                     VLCADMAANWLVRYCDGQLANQPTVAEMDKNMDMMLHFKRVERPAAGVYGGLCVAPYH
                     FKHFDELLADMGATIRRRSPLVEKFTPPDADAYARFLASAPDYKVEAA"
     gene            2771..3580
                     /locus_tag="VGA15_05465"
     CDS             2771..3580
                     /locus_tag="VGA15_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9587176.1"
                     /translation="MKTRDGRLSYEAAGDPALQPLVFLHGIGGAARAWRGQLAAFSDR
                     YRAIAWDMPGYGGSALLPSVSIAALADALRDFLRQVGATTPILVGHSIGGMIVQQLLA
                     KSPHIASAVVLAQTSPAFGKPDGDWQKAFIEARLGPLDRGETLVSLAPTLVKESIGDD
                     PDISGMELARDCMACVPEPTYRATMLALMGFDLRDALKNISVPTLVLSGSKDKNAPAP
                     MMAKMASYIPSATYVELEGAGHLVNLERPATFNAALDRFLKGNVAATQVTA"
     gene            3580..4869
                     /locus_tag="VGA15_05470"
     CDS             3580..4869
                     /locus_tag="VGA15_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9587177.1"
                     /translation="MTMQASKIAKAVDEIDLDAPIFDPNAFRLNDEQAAIIATARALG
                     QTVFASRAAAYDRDAKFPTENYRDLHRAGLLGIAIPKKHGGLGTDYQTYAIAAAEIGR
                     YCGATALTWNMHVCSTLWSGPLADDLDMDASTRADHEQRRAVHYKRIVDDGAIYSQPF
                     SEGGAAAAGGVAFGTEAKPVKGGWIVNGKKIFASLSGHADYYGVLCTEIAEGEKASRR
                     NTLYLAMPAKATGVSVVGDWDPLGMRGTVSRTLLFKDVFVAEDAALMPRGVYFQAAMR
                     WPHMFLTLSPTYVGLAQAAYDFTVRYLRGEVPGTPPVKRRMYPTKQIAVAQMQIKLEQ
                     IKAIWFQAVTEARANPTKEQVLRAYAAQYSAMEGANEIATLAIRTCGGQAMLKSLALE
                     RIYRDSRCGSLMLPWTAELCLDRIGREALYETDESDD"
     gene            4877..>6000
                     /locus_tag="VGA15_05475"
     CDS             4877..>6000
                     /locus_tag="VGA15_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9587178.1"
                     /translation="MDLSDLIERNAAFTPDKPAIVFEGKVLSYAAFGARIEHTARALK
                     AEFGIGRGDRVAILSLNRPDYLVLLYACARLGAMLVPLNWRLAVAEQLFILSDASVKV
                     LVLEQAFAEVLPALEKSLPDTGIIGLDFVPRQGSAFDQVLTQARGGDGRNPDTDLTCP
                     LLIVYTSGTTGRPKGAVLRQEALLWNGVMSQHMHGLTSSDHVLTVLPLFHVGGLNIQT
                     TPALHHGATVTIHPRFTPDAALAAIARERPTLTVLVPATIQAVTDHPDWPSTDLSSMK
                     AVSTGSTIVPPHLIERFAARGVPVLQVYGSTETCPVAVYTRLGGDLSRVGSTGLPGLC
                     CEAAVIDDAGVELPPHTPGEIAVRGPNVFSEYWGNEEATR"
BASE COUNT         1196 a         1852 c         1824 g         1128 t
ORIGIN      
        1 acataggcat aacccttcaa accggcggtc gatacaatcg cgacaatgcg gccatagcgc
       61 cgatccttca tcgcaggcag caccgcctgg accgcgtgaa ccacgcccat gaagttgaca
      121 tccatcattc gccgaaacag cgccgcatcg gattttccga acggcgcgga ttccgcaatg
      181 cccgcattgg caatcaggat atcgatcggc tggcggcctg cggcttccgc aaccgcggcg
      241 ctgacggccg cttgatcggc gacatcggcc acaccagcaa aatgcgcggc gccggcggca
      301 acggcgtcat ccagtgccgc gcgatggcgg ccgagcacgg tgaccgtcgc tcccgcctgc
      361 gcaaggctgg aggcgatcgc gcggccgatg ccccgcccgc ctccggtgac gagcgcgtgc
      421 ggggaatgcg gcagtccgga catgggtagc ctccccgaat gaatgcatcg atgcagagac
      481 ttgcgtatat tttagacttc aagcttttgc aagaaacgtc gatgatgtgt gcttgacaga
      541 tgaagcaccg caggtagcgt cgacttgcca agcaggagga tgggatcatg gcagctctcg
      601 aaaaaggcat cacgcggaac ggaacggggt attccggcaa gacctggaat attcttggac
      661 aggtttattt cccaaaggcg gtgacggact ccacttttgc tttcgagacc aatagcgatc
      721 ccggccagtt tgtcccggtt catattcatc ccacccagga ggaattcatc ctggtccagg
      781 acggcatgct ggacctgaaa ctggatggcg tctgggtaca ggcgaacgcc ggcgatctgg
      841 ttcggctacc ccgcggcatt tcgcacggct atttcaacaa gtcggacaag ccggcgcggg
      901 cgctgttctg ggtatcgccg acgcaaaaac tcgaagcgct gttcaatcag cttcacaatc
      961 tgacggatgt cgctgaaatc gtccgcattt ctgccgagca cgaagtgaac ttcctgccgc
     1021 cggaggcaaa cgactagcgt cccggatccc ggtccagttc actaccgcgc tgtcgccagg
     1081 caactccagg ctggccgcgc atgcccatct gtcgggtccg cggatatctg caggaggaga
     1141 ttaatccatg gtcaagcaga agaatgtttg cattattggt gcaggcgtcg ccggccttgc
     1201 cgcagcaaag atattcgacg ctcgcggaca caaggtgata atcctcgagc gcagcggcga
     1261 tcttggcggc gtctgggagc cggcgcgatc ctatcccgag gttcaaactc aaagccccaa
     1321 ggatctctac cgctatactg acaagccgat gccgaactcg tatccggaat ggccgacagg
     1381 accacaggtt catgcctacc tgaccgatta cgccggggag cacggcttga tgcaccggat
     1441 gcgcctcaat actaccgtag tcgggatggc gcgcaggaat gcggacctgc cgggctggac
     1501 gctggaaatc cggaatccat atggcggtgt gactcacgag gaatttgact ttgtcgccgt
     1561 ctgcaccggc cagttcaacg aacctcaggt gatcagtcac cccggcgaag atgccttcaa
     1621 ggccgcaggc ggcaggatcc tgcattccgc gcagcatacc gactcgtcct tcgtcaaagg
     1681 aaagaaggtc gtcatccttg gcggctcgaa atcagcaacc gatattgctg tcaattccgt
     1741 taacgccggc gcgagcgaag tcacgctggt ctatcgcgag cccgtctggc gaatcccgta
     1801 cttcatcgga gggctcctca atttcaagcg gatactctac acccgcgccc aggaggaaat
     1861 gtttcgcagt tggggcatcg gtggcatttc ccgcgctgcc catgccatcg gcaaaccgct
     1921 ggtatgggcg aactggcgag cgctcgaaag tcttctcaag ctgcaattga agctgaagaa
     1981 gtgtggaatg gtaccgaaga cgcggatcga ggacggagtt aactgctcgg tgccgattgc
     2041 aacgccggaa ttctttccga tggtggccga cggccggatc aaagccattc gcggcacatt
     2101 cgaccgttac gacggcaaga caatcatcat gaccggcgga gagcgagttg cggttgacat
     2161 cgccatactc gcgattggct tcaagcttgg cgttccgttt ctgcccgagg cctaccgcaa
     2221 caagctcgtg gaacctgacg gccaatatcg gctgtaccgt ctgatcgcca atcccgatct
     2281 tcccgacatg ggtttcgtcg gcttcaattc tagcttttgc acggtgctct gcgccgatat
     2341 ggccgcaaac tggctggtgc ggtattgtga tggtcaattg gcgaaccagc cgaccgtagc
     2401 cgagatggat aaaaatatgg acatgatgct gcacttcaag cgcgtcgagc ggccggccgc
     2461 aggcgtttac ggcggactgt gcgtcgcgcc ctaccacttc aaacatttcg acgaattgct
     2521 ggccgacatg ggtgcaacga tacgtcggcg cagcccgttg gttgagaaat tcacgccgcc
     2581 tgacgccgac gcctacgcac gtttcctggc ctcagcacca gactacaagg ttgaggcggc
     2641 gtagcaggcg agccgctcgc cggtccaaaa ttgctttaaa tataaaatat ctctttttcg
     2701 cgcgttgagg tcctgttagc atcggcaggt ccaaaactac cggaggtcag gcgtgcccgc
     2761 ttctacgccc atgaaaacga gggatggccg tctcagttat gaggcagccg gagatcctgc
     2821 cttgcagccg ctggtgttcc tgcatggaat cggcggcgcg gcgcgggcgt ggcgtggcca
     2881 actcgccgcc ttcagcgatc gataccgcgc catcgcatgg gacatgccgg gctatggcgg
     2941 ctcagcatta cttccatccg tcagcattgc cgccctcgcc gatgccttgc gggatttcct
     3001 gcggcaggtc ggagcgacga cacccatcct tgtcggacac tcgatcggcg gcatgatcgt
     3061 tcagcagttg ctggcgaaat cccctcatat cgccagcgcc gtcgtgctcg cacaaaccag
     3121 ccctgcattc ggcaaacccg atggcgactg gcagaaggcg ttcattgagg cgcggctcgg
     3181 cccgctcgat cgcggcgaaa cgctcgtctc actggcgccg acactggtga aggaatcgat
     3241 cggtgacgat cccgacatca gcggcatgga gcttgcccgc gactgcatgg catgcgttcc
     3301 cgagccgacc taccgcgcca cgatgctggc gctgatgggg tttgatctgc gggatgcgct
     3361 caaaaatatc tcggtgccga cgttagtgct gtcgggatcg aaggacaaga atgcaccggc
     3421 gccgatgatg gcgaagatgg ccagctatat tccatccgcg acatacgttg agctcgaagg
     3481 cgccggtcac ctcgtcaatc tggaacgtcc cgcgacgttc aacgccgcgc tcgatcgatt
     3541 tctgaaaggc aatgtggctg caacacaagt gacggcatga tgacaatgca agccagcaaa
     3601 atcgcgaagg cggtcgacga aatcgatctg gatgcgccga tcttcgaccc caatgcgttc
     3661 cggctgaacg acgagcaggc tgcgatcatc gcgacggcgc gcgcgcttgg ccagaccgtt
     3721 ttcgccagcc gcgcggcggc ctatgatcgc gatgcaaaat tcccgaccga gaactacagg
     3781 gatctgcatc gcgcaggctt gctcggcatc gctattccga agaaacatgg cggcctcggc
     3841 accgactacc agacctatgc gatagcggcg gccgaaatcg gccgctattg cggcgcgact
     3901 gcgctgacct ggaacatgca tgtatgttcg acgctgtggt ccggcccgct ggccgacgat
     3961 ctcgacatgg acgcatcaac acgcgccgat cacgagcaac ggcgcgccgt gcactacaag
     4021 cgcattgtcg acgacggcgc gatctattcg cagccgttct cggaaggcgg ggcggcggca
     4081 gccggtggcg tcgcttttgg gacggaggcg aaaccggtca aaggtggctg gatcgtcaac
     4141 ggcaagaaga tctttgcgtc gctttcgggc cacgccgatt attacggcgt gctctgcacc
     4201 gagatcgcgg aaggtgaaaa ggcctcccgc cgcaatacgc tctacctcgc gatgccagcc
     4261 aaggccaccg gcgtatccgt ggtcggcgac tgggatccgc tcggcatgcg cggcacggta
     4321 tcgcgcaccc tgctgtttaa ggatgtgttc gtggcggagg atgcggcgct gatgccgcgc
     4381 ggcgtctatt ttcaagcggc gatgcgctgg ccgcatatgt tcctgacgct gtcgccgact
     4441 tatgtcggcc tggctcaggc ggcttacgat ttcaccgtgc gctatttgcg cggcgaggtg
     4501 ccgggcacgc ctccggtcaa gcgccggatg tatccgacca agcagatcgc ggtggcacag
     4561 atgcagatca aactcgagca gatcaaggcg atctggttcc aggcggtcac tgaagcccgc
     4621 gccaatccga ccaaggagca ggtgctgcgc gcctatgccg cgcaatattc ggcgatggaa
     4681 ggcgccaacg agatcgccac gctggcaatc cgcacctgcg gcggccaggc gatgctgaaa
     4741 tcgttagccc tggagcggat ctaccgcgac agccgttgcg gttcgctgat gctgccgtgg
     4801 accgccgaac tgtgcctcga ccgcatcggc cgcgaggcgt tatacgagac cgacgaatcg
     4861 gacgactagt tcgaaaatgg acctttccga cctcatcgaa cgcaacgcgg cattcacgcc
     4921 ggacaaaccc gcgattgtat ttgaaggaaa ggtcctgagt tacgcggcgt tcggcgcgcg
     4981 catcgaacac acggcacgcg cgctgaaggc cgaattcggc atcggccgtg gcgatcgtgt
     5041 tgcgatcctc agcctgaacc ggcccgacta tctcgtattg ctctatgcct gcgcgcgcct
     5101 cggcgccatg ctggtgccgc tgaactggcg gcttgcagtg gccgaacaat tgttcatcct
     5161 gtcggacgcc tcggtaaagg tgctggtgct cgaacaggcc ttcgcggagg ttctgcccgc
     5221 gctggaaaaa agcctgccag acactggcat catagggctc gactttgtac ctcggcaagg
     5281 aagcgcattc gaccaggtgc taacgcaggc gcgaggcggc gacggccgca atccagatac
     5341 cgatctgacc tgcccgttgc tgatcgtcta tacctccggc acgaccggaa gacccaaggg
     5401 cgccgtgctg cgccaggaag ccctgctgtg gaacggagta atgagccagc atatgcacgg
     5461 cctcacctcc agcgatcatg tgcttaccgt gctgcccttg ttccacgtcg gcggcctcaa
     5521 catccagacc acgccagcgc tccaccatgg cgcgaccgtg acgatccatc cgcgattcac
     5581 gcctgacgcg gcgctcgcgg caatcgcgcg cgagcgtccc accttgacgg tgctggtgcc
     5641 ggcgaccatc caggcggtga ccgatcatcc cgactggccc tcgaccgatc tctcctccat
     5701 gaaggcggtc tcgaccggat cgaccatcgt gccgccgcat ctgatcgagc gcttcgcggc
     5761 gcgcggggtg ccggtacttc aggtctatgg ctcgaccgaa acctgcccgg tcgcggtcta
     5821 cacccgcctc ggcggcgatc tttcgcgcgt ggggtcgacc ggcctccccg gcctgtgctg
     5881 cgaggcagcc gtgatcgatg atgccggcgt cgagctgccg ccgcacacgc cgggtgaaat
     5941 cgcggtgcgc ggtcccaacg tgttctcgga atattggggc aatgaggaag caacgcggga
//
LOCUS       DASWGM010000178         7769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2093280,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000178 DASWGM010000000
VERSION     DASWGM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093280"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VGA15_05480"
     CDS             <1..650
                     /locus_tag="VGA15_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9587179.1"
                     /translation="AVKDFLAYLETLVARRRTKPGSPERDVLTRLIQGEDNGERLTGK
                     ELLHNCIFLLNAGHETTTNLIGNGLVGLSENPAEKKRLIENPGLIKTAVEEILRFESS
                     NQLGNRMTTAQVELSGITLAAGTPVTLCIGAANRDPAQFAKPEHLDIGRSPNRHLAFG
                     TGAHQCAGMALARLEGAIAMSRFLARFPDYALNGPPVRGGRVRFRGFLSVPCTVS"
     gene            689..1222
                     /locus_tag="VGA15_05485"
     CDS             689..1222
                     /locus_tag="VGA15_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9587180.1"
                     /translation="MTSTTTQPGAAIPPDDANNKLTVANPDDPKLRHISVAGGTYTIL
                     VSGEQTGGRYCLIDMLVPAGGGPPPHRHDFEEMFTILDGEIELTFRGGVQRASTGSTV
                     NIPANAPHSFRNMSDKPARLLCMCTPAGQEEFFMAVGDPVDSRIAPPPLLSKAEQAER
                     IQRAKALSAKYRTELLV"
     gene            complement(1315..2145)
                     /locus_tag="VGA15_05490"
     CDS             complement(1315..2145)
                     /locus_tag="VGA15_05490"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX9587181.1"
                     /translation="MTMPSLFLSHGAPTLPLSDTPARAFLNELGDLFARPKAILVISA
                     HWETSVPTVGAVDRNETIHDFGGFPRALYELRYPASGSPSVAARVSDLLLAAEFDCNI
                     DRRRGLDHGAWVPLLLMYPQADIPVLQLSVQPHLGPKHHLLVGRALAPLRKEGVLIIG
                     SGSLTHDLSEFRGHGPNDPAPDWVNGFADWFNCALTAGQTDDLLDYRRQAPFAIKNHP
                     SEEHLLPLYAALGAAGRNARAERLHASSTYSVLRMDVYAFQASSDLMRNEYSETKSLV
                     "
     gene            complement(2225..2665)
                     /locus_tag="VGA15_05495"
     CDS             complement(2225..2665)
                     /locus_tag="VGA15_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9587182.1"
                     /translation="MIDSRTAPYAALLLRVSLGMLFLAHASLKLFVFTPAGTAKFFGS
                     VGLPPEFAYVVITAEVLGGIALILGIWTRIVAIAATPILLGAIFTVHGAAGFFFTNAH
                     GGWEYPAFWTIALVVQALLGDGIYAVRVPRAGGFAGRLTDAHSH"
     gene            2766..3707
                     /locus_tag="VGA15_05500"
     CDS             2766..3707
                     /locus_tag="VGA15_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9587183.1"
                     /translation="MLDRLTGLEVFAKVAAAGSLSSAARAIGMSQTMVTKHIAALETR
                     LGVKLLHRTTRRLSITEAGRSYLEASERILAEFEAANAAVAADRVEPRGLLRLNVPVS
                     FGTRQIAPLLAEFAKRHPLVTVELGLNDRLVDLAEEGWDLAIRIGNLSDSSLIARRIA
                     PCRMVVCAAPSYLQARGDPRTVSSLAGHNCLGYTLSRLSGVDRWMFGARADVSIQVSG
                     NLRANNGDALRAAAIAGQGLIYQPTFIVADDLRAGTLVALSMDQPTVQFSGIYAVFPP
                     DRHPVAKVRAFIDFIATRFTPEPPWDRDLAPPQKLTP"
     gene            3927..4739
                     /locus_tag="VGA15_05505"
     CDS             3927..4739
                     /locus_tag="VGA15_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
                     /protein_id="HEX9587184.1"
                     /translation="MPAREHEPTRVLESAQIRQFIQDGFVRIDRAFPRQLADQGRAIL
                     WRDLPCDPDDPASWTRPVIRLGYYGDEPFKTAANSPLLHAAFDQLVGKGRWRPRTNLG
                     TFPVRFPSPDDPGDAGWHVDVSFPGEDCDPNEQTDFSSWRVNVTSRGRALLMLFLFSD
                     VGERDAPTRIRVGSHFDMARFLAPAGEAGMSHLVLDQMGAERPEALATGEAGTVYLCH
                     PFLVHAAQMHRGSEPRFMAQPPLHPAEPLQLDRVNGDYSPVEMAIRQALRRS"
     gene            complement(4754..6346)
                     /locus_tag="VGA15_05510"
     CDS             complement(4754..6346)
                     /locus_tag="VGA15_05510"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX9587185.1"
                     /translation="MALLRDPLLNKGTAFTEQERDTLGLRGFLPAHVFSMKAQAERVL
                     VNLRSLPSDLAKYVALNELHDRNEALFFRVVADNIDEIQPLIYTPTVGLACQRFGYIF
                     QRPRGLFIAANDRGHIAELLGNWPYPAKLIVVTDGERILGLGDLGANGMGIPVGKLSL
                     YSACAGIHPELCLPVVLDVGTNNEALLNDPYYIGTRQHRLGGAAYDEFVDEFITAARE
                     TFPGVVIQFEDFANHSAFRLLHKYRDRIPVFNDDIQGTAAVALAGLLSALRVTGSKLT
                     DQTLLFLGAGEAATGIADLVVSAMVAEGASEAEALRRNWLVDSRGLVVRNRPGLTEHK
                     LRYAHDQAPIGDFLTAIQTLKPTAIIGVAAVGGAFTPEVLQTMAGINDRPIVFALSNP
                     TSKAECSAEEAYYHSGGRALFACGSPYDPVQFNGTTFVPRQGNNSYIFPGVGLGAIAS
                     GSRLVTDEMFMAAARTLAQLVSESDIKQGSLYPALPRIREVSAHIAAAVAGVAYKRGL
                     ATGQLPKDLVAYIQSQMYDPHY"
     gene            complement(6720..7397)
                     /locus_tag="VGA15_05515"
     CDS             complement(6720..7397)
                     /locus_tag="VGA15_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX9587186.1"
                     /translation="MDFEELVNTRRSVRGFRAEPVPRSVIEEIIEVAKRAPSSMNTQP
                     SHVHVLTGEPLEQVRRRNMEEMLAGAKPKRDIVSHGEYQGVHRQRQVDIAKQLFAVMG
                     IARDDKPMRQDWVSRGFRQFDAPVSLVLTYDRILDPGAVCHFDLGALCYGIVLAAWNR
                     GLGSVINGQGITRSDIVREVADIPEDQAIMTCVAMGYPDDSFPANAVTSDRQPNSDFV
                     RYVGFHD"
     gene            complement(7544..>7769)
                     /locus_tag="VGA15_05520"
     CDS             complement(7544..>7769)
                     /locus_tag="VGA15_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593714.1"
                     /note="YghU; B2989; one of eight putative glutathione
                     transferase proteins from E. coli; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEX9587187.1"
                     /translation="KGLLYGGGEFLGVQEYANVQRWTDAIAKRPAVRRGRMVNRTWGE
                     LSSQLHERHDAGDFETKTQDKIEESKTAVV"
BASE COUNT         1514 a         2417 c         2383 g         1455 t
ORIGIN      
        1 aagcggtgaa ggattttctc gcctacctgg aaacccttgt tgcgcggcgg cgaacaaaac
       61 ccggtagtcc cgaacgcgat gtgctgacgc gcctcattca gggcgaggac aatggcgagc
      121 gtctgaccgg aaaggagttg ctgcacaact gcatcttcct gctcaacgcc gggcatgaga
      181 ccaccaccaa cctgatcggc aacggactgg tggggttgtc ggaaaatccc gccgagaaaa
      241 aacggctgat cgaaaatccc ggtctgatca agaccgcggt cgaggaaatt ctgcgatttg
      301 aaagctcgaa ccagctcggc aaccgcatga cgacggcgca agtcgaactc agcggcatca
      361 cgctggcagc cggtacgccg gtgaccctgt gcattggtgc tgccaaccgc gatcccgcgc
      421 aatttgccaa accggaacat ctcgacatcg gccgcagccc caaccggcat ctcgccttcg
      481 gcaccggcgc gcatcaatgc gccgggatgg cgctggcgcg gctcgaaggc gccattgcga
      541 tgtcgcgatt cctggcgcgg tttcccgact acgcgctgaa cggaccgccc gtgcgcggcg
      601 gccgcgttcg ctttcgcgga ttcttgagcg tgccgtgcac cgtcagctag tcttgcctat
      661 gctgccattc aacaaaagga aacatctgat gaccagcacg accacccagc ccggagcagc
      721 gatcccgcct gatgacgcca acaacaaact cacggtggcc aatcccgacg atccgaagtt
      781 gcgccacatc tcggtcgcgg gcggcaccta cacgatcctc gtgagcggcg agcagaccgg
      841 gggccgctac tgcctgatcg acatgctggt gccggcgggc ggcggcccgc cgccgcatcg
      901 tcacgatttt gaggagatgt tcacgatcct ggacggcgag atcgagttga ccttccgcgg
      961 cggggtccag cgggcgagca ccggctcgac cgtgaacatc ccggccaacg cgccccactc
     1021 cttcaggaac atgtcggaca agccggcacg cctgctctgc atgtgcacgc cagcgggaca
     1081 ggaagagttc ttcatggcgg tcggcgaccc cgtcgacagc cgcattgcgc caccgccgct
     1141 gctcagcaag gccgagcagg ccgagcgcat ccaacgcgcc aaggcgcttt cggccaagta
     1201 tcgaaccgag ttgttggtgt gaaggtcctc gctctgggcg cgaccggtgg caccggtcgc
     1261 gagatcgtgc gtgaggcggg cgcagggatt agttattttg aatgatcccg cccttcagac
     1321 caatgacttc gtttccgaat attcgtttcg catcaaatcg ctcgatgcct gaaacgcata
     1381 gacgtccatc cgcagaacgc tgtaggtcga acttgcgtgc agccgttcgg cgcgcgcgtt
     1441 tcttcccgcg gcgcccaatg ccgcgtacag gggcaaaagg tgctcttcgc ttgggtggtt
     1501 cttgatcgcg aacggcgcct gtcgccgata gtcgagcaga tcatcggttt ggcccgcggt
     1561 gagcgcgcaa ttgaaccagt cggcaaaacc gttgacccaa tcgggtgcag gatcgttcgg
     1621 accatggccg cgaaattccg acaaatcgtg cgtcaggctg ccgctgccga tgatcaacac
     1681 gccctctttc cgcagtggcg ccagggcgcg tcccacgaga agatggtgtt ttggtccaag
     1741 gtgcggctgg accgagagtt gcagaaccgg aatgtcggct tgcggataca tcagcaacag
     1801 cggcacccag gcgccgtgat ccaggccacg gcgacgatcg atgttgcagt cgaattcggc
     1861 cgcgagcagc aggtctgaaa cgcgcgcggc aacggacggc gagccggaag ccggatagcg
     1921 cagctcatag agcgcccgcg ggaatccgcc gaagtcgtgg atggtctcgt tccgatccac
     1981 ggcgccaacc gtcggtaccg atgtctccca atgtgccgat atcacaagaa tggccttggg
     2041 acgcgcgaac aggtcgccga gctcattcag aaacgcgcga gctggtgtgt cgctaagcgg
     2101 caaggtcggc gcgccgtgcg acaggaacag gcttggcatg gtcatgacac gttgtccgat
     2161 acgcttggtc tgatcatgca gggccggaat acggccccgc tgcctcgtcc aataggcacc
     2221 gcgttcaatg cgaatgcgcg tcggtcaacc ggccggcgaa accaccggca cgcggcacgc
     2281 gcaccgcata gatgccatcg ccgagcaatg cctggacgac caacgcgatg gtccagaacg
     2341 ccggatattc ccagccgcca tgagcgttgg tgaaaaagaa cccggcggca ccgtgcacag
     2401 tgaaaatggc gccgagcaaa atcggcgtgg cggcaatcgc aacgatccgg gtccagattc
     2461 caaggatcag cgcgatgccg ccaagcactt ccgcagtgat gacgacatag gcgaactcgg
     2521 gcggcagtcc gacgctgccg aaaaacttcg ccgttccggc cggcgtgaag acgaacaatt
     2581 tgaggctggc atgcgcgaga aagagcatgc ccaggctgac gcgtagcaac agcgcggcat
     2641 agggagcggt tcgggaatcg atcatggttg tctcctgatt ggtcggtgat ctatgaccta
     2701 ttcttctgag tgggataatc tggcaattcg gaaatacaga tcacacccag agagtgagat
     2761 aagccatgtt ggaccgcctg acaggcctcg aggtattcgc caaggtcgct gctgccggaa
     2821 gcctgtcgtc tgcagcgcgg gccattggca tgtcgcagac catggtcacc aagcatattg
     2881 ccgcgctgga gacgcgactg ggcgtcaagc tcttgcaccg caccacgcgg cggctgtcga
     2941 tcaccgaggc cgggcgcagc taccttgaag cctccgagcg catccttgct gaatttgagg
     3001 ccgccaatgc agccgtcgcg gcagatcggg tcgaaccaag ggggctgctc cggctcaatg
     3061 ttccggtctc gttcggcacg cggcagatcg ccccattgct cgccgaattc gcaaaacgcc
     3121 atccgctcgt caccgtcgag cttggcctga acgatcgttt ggtcgacctt gccgaagagg
     3181 gctgggatct tgcgatccgg atcggcaacc tcagcgattc aagcctgatc gcgcgacgca
     3241 ttgcgccctg ccggatggtg gtatgtgcgg cgccgtccta tctccaggcg cgaggcgacc
     3301 cgcgcacggt ttcgagcctg gcgggtcata attgtcttgg ctacaccctg tcccggctaa
     3361 gcggcgtcga ccgatggatg tttggcgctc gcgctgacgt cagcattcag gtttcgggca
     3421 atctgcgcgc caacaatggc gatgccctgc gggccgccgc gatcgcggga caaggtctca
     3481 tctaccagcc gacgttcatc gtcgccgatg atttgcgcgc gggcacgctg gttgctctct
     3541 cgatggatca gccgaccgtc cagttcagcg gtatctatgc cgtgttcccg ccggatcgcc
     3601 atccggtcgc caaagtgcgc gcgttcatcg actttatcgc gacccgcttt acacccgagc
     3661 cgccctggga tcgtgacctc gctccgcctc aaaagttgac cccttgagcg caccgatgat
     3721 aatttatggc catgatgacg aataacgcat ccaatatcgc ttcggtcttc ttcctccgcc
     3781 gctaacggcg ttgcctgcct cgcgcaggcg gaagacggca gtgtgcccag cggccacgcg
     3841 cccccgcgca ttgtcaccga ccgaaccagc cgaccaactc gcttcgcgcg agtgatgagt
     3901 gttggccaag cgatatgagg attttcatgc ctgcccgtga acatgaaccc acgcgcgtgc
     3961 tcgagagcgc gcagatacgg caattcattc aggatggctt tgtcaggatt gatcgtgcct
     4021 ttccccggca acttgccgac caaggccgcg ccatcctgtg gcgcgatctg ccgtgcgatc
     4081 cggatgatcc ggcgagctgg acacggcccg ttatccggct tggttactac ggtgacgagc
     4141 cgttcaagac ggccgccaac agtcccctgc tccacgcggc ttttgaccag ctcgtcggaa
     4201 aaggacgctg gcgtccacgc acgaacctcg gaacctttcc ggtgcggttt ccgagcccgg
     4261 acgatcccgg cgatgccggt tggcatgtcg acgtcagctt ccccggcgaa gattgcgatc
     4321 cgaatgagca gacggacttt tcctcttggc gcgtgaacgt aacttcacgg gggcgcgcac
     4381 tgctaatgct gttcctgttc tccgacgttg gcgagcgaga cgctccgacg agaatacggg
     4441 taggctcgca ttttgacatg gcgagatttc ttgctccggc cggcgaggcg ggcatgtcgc
     4501 atttggtcct ggaccagatg ggggccgagc gaccagaggc tttagccacg ggcgaagctg
     4561 gcaccgtcta cttgtgtcat ccttttctcg tgcatgccgc gcaaatgcac cgtggctcgg
     4621 agccgcgttt catggcgcag ccgccacttc atccggccga gccgttgcag ctcgatcggg
     4681 tgaacggcga ttattctccg gtcgagatgg ccatccggca ggctttgcgc cgcagctagc
     4741 taaagcgcac cattcagtaa tgcgggtcgt acatttgcga ctgaatgtag gcgacgagat
     4801 ccttagggag ctggccggtc gcaagaccgc gcttgtaagc gacgccggcg acagccgccg
     4861 cgatgtgagc ggaaacctcg cggatccgcg gcagagccgg atagaggctg ccctgcttga
     4921 tgtcggattc gctgacgagc tgcgccagcg tgcgggcggc cgccatgaac atctcgtcgg
     4981 tcaccagcct gcttccgctc gcgatcgcgc caagaccgac gccggggaag atgtaggagt
     5041 tattcccctg gcgcggcacg aacgtggtgc cattgaactg caccggatcg taggggctgc
     5101 cgcacgcgaa cagcgcacga ccgccgctat ggtaataggc ttcctcggca gagcattccg
     5161 ccttcgaggt gggattggag agcgcgaaca cgatcggccg gtcgttgatc cctgccatgg
     5221 tctgaagcac ctcaggcgtg aaggcgccgc cgaccgccgc aaccccgatg attgcggtgg
     5281 gtttgagcgt ctggatcgcc gtcaggaaat cgccgatcgg cgcctgatca tgcgcgtagc
     5341 gaagtttgtg ctcggtaagg cccggccggt tcctgaccac caggccacgc gagtcgacga
     5401 gccagttgcg ccgaagtgcc tcggcttcag atgcgccttc cgccaccatg gccgagacca
     5461 cgaggtcggc aatgccggtc gcagcctccc ctgcgcccag gaaaagcagt gtttgatcgg
     5521 tcagtttgct gccggtcacg cgcaaggcgg aaagaaggcc cgccagcgcc acggcagccg
     5581 ttccctggat gtcgtcgttg aagacgggaa tcctgtcgcg atatttgtgc aacaggcgga
     5641 acgccgagtg attggcgaaa tcctcgaact ggattaccac gccggggaaa gtctcgcgcg
     5701 ccgcggtgat gaattcgtca acgaactcgt cataggcggc gccgcccaga cggtgctggc
     5761 gcgtgccgat ataataggga tcgttcagca acgcctcgtt gttggtgccg acgtccagca
     5821 ccaccggcag gcaaagctcc gggtggatgc cggcgcaggc ggaataaagc gaaagcttcc
     5881 cgaccgggat gcccatgcca ttggcaccga gatcaccgag gcccaggatg cgctcgccgt
     5941 cggtgacgac gataagtttt gcgggataag gccaattgcc gagcagttcg gcgatgtgtc
     6001 cgcgatcgtt agctgcaatg aacaggccac gcggccgctg gaagatgtag ccaaaccgct
     6061 ggcaggccag gcccaccgtc ggcgtataga tcagcggctg gatttcatcg atattgtcgg
     6121 cgacgacgcg gaagaacagc gcctcgttgc ggtcgtgcag ttcgttgagc gccacgtatt
     6181 ttgcaaggtc gctgggcagg ctgcgcagat tgaccagcac acgctcggcc tgagccttca
     6241 tcgaaaacac gtgcgccggc aaaaagccgc gcaatcccag cgtgtcgcgc tcttgctcgg
     6301 tgaaggcggt acccttgttg aggagcggat cgcgcaacag cgccaaaccg cgcggcgaat
     6361 cgaaaggctg tgggcgggga ttcgcccgca ctgacacgct catgatattc cccccgaagc
     6421 cggtttgttg tttggccccg cagcgtccgc cattcgaagc agaaaaagtt gatcgaaatc
     6481 aaccgcagga gaaactgtct cctggctccc agcgcatcca cggggtcatc ggattacttc
     6541 cgatattgac acctttcgga cctgacgtga agcctgactt gagtccgcca agtgttcaaa
     6601 aaagaacatc ttcatgaatc catgccaaac ctccgagcga tgctgctctg caaaatctca
     6661 tccatcagtc aggctcgggc tttcggaaga ttgcgcgtcg tctcggagcc ggtcatatcc
     6721 taatcgtgaa agccgacata gcgaacgaaa tcgctgtttg gctgccggtc ggaggtgacg
     6781 gcattggcag gaaaactgtc atcaggatat cccatggcga cgcacgtcat gatagcctgg
     6841 tcctcgggga tgtcagcgac ttcgcgcacg atatccgatc gggttatgcc ctggccattg
     6901 atgacggagc cgaggccccg attccaggcg gcgagaacga tgccatagca gagagcgccg
     6961 agatcgaagt ggcagaccgc gccgggatcg aggatcctgt cataggtcaa caccagcgag
     7021 accggcgcat cgaactgccg gaatcccctt gacacccagt cctgccgcat cggcttgtcg
     7081 tcacgtgcaa ttcccatcac ggcaaacagc tgcttggcga tgtcgacctg ccgttggcga
     7141 tggacgccct gatattcgcc gtgcgataca atatctcgct ttggcttggc gccggcgagc
     7201 atctcttcca tgttgcgccg gcggacctgc tccagcggct ctcccgtcaa gacatgcaca
     7261 tgcgagggtt gggtgttcat ggaagacggc gcgcgcttgg cgacctcgat gatctcttcg
     7321 atcacggagc gcggcaccgg ttcggctctg aaaccacgca cacttctgcg cgtgttgacg
     7381 agttcctcga aatccatttc gggtatcctc aaacgtaggg tgggcaaagg cgcccttcgc
     7441 gccgtgccca ccaacttcta atgcgctatt atggtgggca cgcggagttt atcatcgggc
     7501 gcgcattcgc gcgacccgtt ggctttgccc accctacaat ttctcaaacc accgcggtct
     7561 tgctctcctc aatcttgtct tgcgtcttgg tttcgaaatc gccagcgtcg tgacgttcgt
     7621 gcagctggct tgaaagctca ccccaggtgc ggttgaccat gcgcccccgc cgcacggccg
     7681 gacgcttggc gatggcgtcg gtccagcgct gcacgtttgc gtactcctgc acgcccaaga
     7741 attcgccccc accatagagc aggcccttc
//
LOCUS       DASWGM010000179         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1679395,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000179 DASWGM010000000
VERSION     DASWGM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679395"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /gene="mepA"
                     /locus_tag="VGA15_05525"
     CDS             complement(<1..704)
                     /gene="mepA"
                     /locus_tag="VGA15_05525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX9587188.1"
                     /translation="MNLRLIPLLLLISVALASATIALAQDKGSVDPTPLPPLANPNDP
                     KLGAKELFGRKVLPAAKPTRVVGFYAKGCIAGAKALPINGETWQVMRLSRNRNWAHPD
                     MVALLERLSAKAHKDAGWPGILVGDMSQPRGGPMFTGHASHQVGLDADVWLTPMPDRQ
                     LSRNEREEMSAVMMVRSDRLDIDPHAWTPNHLSVIRDAAREPSVQRIFVNAAIKKALC
                     REAKGDRSWLSKVRPM"
     gene            complement(1254..1478)
                     /locus_tag="VGA15_05530"
     CDS             complement(1254..1478)
                     /locus_tag="VGA15_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587189.1"
                     /translation="MRTISPISTGARRLLSESFGLGFAGRPDILVVAACLIFFAVLGR
                     IFGIAASCSLPALGLFSDNYLKKRYLFAGR"
     gene            1393..>3458
                     /locus_tag="VGA15_05535"
     CDS             1393..>3458
                     /locus_tag="VGA15_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9587190.1"
                     /translation="MSGRPAKPRPKLSDSRRRAPVEIGEIVRKRAEAEAAIADARKSH
                     ERLREAIDILPQGIVFLDADGRYILWNKKYAEIYNRSSDLFKPGARLEDTIRIGVERG
                     DYPEAIGREEEWIAERLAKLYHPGERHEQILADGRCILIEERLTEDGGIIGLRVDITE
                     LKQREASFRLLFDSNPVPMIVCAVDEERILGVNAAAIKHYGYSRAEFVKLTIRKLQAF
                     ESEPPWAGDRSSDEQAARTWKHVRADGTLIDLAIYSRRLLYGDRPAVLLALMDITERK
                     RAEARLAFMAQHDGLTGLPNRNLLRQRMDEILQHTRRSADKVAVLVLGLDHFKAVNDT
                     LGHGAGDKLLRAVAKRLRSTLREEDALARLNSDEFAIVQSGLARPEDAVLLCKRLLEA
                     IGDPFLLDGHSVVVGASIGIAMAPSDGDESEKLLKNADMALSRAKNDSRGTFSFFEAG
                     MDARAQSRRKIEIDLRDAIQNDVLRPDYQPLIDLSTGRITGFEALMRWPHSERGMISP
                     AEFIPVAEETGLINALGGLMLRRACMDAALWPDDVRVAVNLSPLQFRAGNLLSVVMDA
                     LKHSGLPARRLELEITETLLLEKSGQVLATLHALRALGVRISMDDFGTGYSSLSYLRS
                     FPFDKIKIDQSFVRDLGANRDAQAIVRSIISLGIGLGVTITAEGVETEAELSCLRAEG
                     CHEGQG"
BASE COUNT          641 a         1089 c         1108 g          620 t
ORIGIN      
        1 tacatcggcc gcaccttgga gagccagctg cggtcgccct tggcttcccg gcacagcgct
       61 ttcttgatcg cggcgttgac gaagatgcgt tgcacgctcg gctcccgcgc cgcgtcccgg
      121 atcacggaga gatggttcgg cgtccaggcg tgcggatcga tgtcgaggcg atcggaacgc
      181 accatcatga ctgcggacat ttcctcgcgt tcgttgcgcg acaattgccg gtccggcatc
      241 ggtgtcagcc agacatcggc atccaggccc acctgatggc tggcgtgccc ggtgaacatc
      301 ggaccgccgc gcggctgcga catgtcgccg accagtatcc ccggccagcc cgcatccttg
      361 tgcgctttag ctgacagccg ctccagcagt gcgaccatgt cgggatgcgc ccaattgcga
      421 ttgcgcgaca accgcatcac ctgccaggtc tcgccattga tcggcagcgc tttcgcgccg
      481 gcgatgcagc ccttggcgta gaagccgacc acgcgcgtcg gcttcgccgc gggcagcacc
      541 ttgcggccga acagctcctt cgcgccgagt tttggatcgt tgggattggc cagcggcggc
      601 agcggcgtgg gatcgacgct gcccttgtcc tgcgccagcg cgatggtggc actggcgagc
      661 gcgacggaga tcaggaggag gaggggaatc aggcggaggt tcattggatc actctgtaac
      721 gcaagtctag cacggaatca gcaagcggcg ataaggcgag ccaatcgcaa tcgcttgatg
      781 tcgcgcgccc tttttctcgc gaacggtcga tccgtcgcgc gttgcacgca accgcgacga
      841 catgcgcggg ctcatggtcc aggtcgtttg catccaggtc gttgcgcgac tggattccgc
      901 accgatggct gcggtacgct gcaccctcgg cgtgcggcgg cgctgattgc atgggtcgag
      961 cgcaacgggc gatgccatca gcgggtcgag atttcgaatg agcatggcac cacgggccgc
     1021 agccttaacg cttcgctaac cttgtcgatc attgatgcga tctcgtgcgc cgatgagaac
     1081 ctcgcgcatt gattgtgcag tcgggacgtt cgaagaccat tttgccttcg catacgcgca
     1141 tgtgaccgat cacacgccgt tacaccgccg ttgtgtacgc cgatgtccgc ggcttcgcct
     1201 cttaagcagg caatcggaac tgaaatcacc ttggtgaggc ccggcggccg gcttcatcgg
     1261 ccggcaaaga gatacctttt tttcagatag ttatcggaga acaggccgag cgcaggaagc
     1321 gaacaggacg cagcaatgcc gaaaatccgg cccaagaccg caaagaaaat caagcatgcg
     1381 gcgaccacga gtatgtccgg tcggccggcg aagcccagac caaaactttc ggacagcagg
     1441 cgccgcgcgc cggtggaaat cggcgaaatc gtgcgcaagc gcgcagaggc cgaggccgcg
     1501 attgccgacg cgcgaaaatc ccacgagcgc ttgcgcgagg cgatcgatat cctgccgcag
     1561 ggcatcgtgt ttctcgacgc cgacggacgc tatattctct ggaacaagaa gtatgcggag
     1621 atctacaacc gcagctcgga cctgttcaag ccgggcgcgc gcctggagga taccatccgc
     1681 atcggcgtgg agcgcggcga ttatcctgaa gcgatcggtc gcgaagagga gtggatcgcc
     1741 gagcggctcg ctaagcttta tcaccccggc gaacgccacg agcaaatcct ggccgacggt
     1801 cgctgcatcc tgatcgaaga gcggctgacc gaggatggcg gcatcatcgg tctgcgcgtc
     1861 gacatcaccg aactgaagca gcgcgaggcc tcgtttcggt tgctgttcga cagcaatccg
     1921 gttcccatga tcgtctgcgc ggtggacgag gaacgcatcc ttggcgtcaa cgccgccgcg
     1981 atcaagcatt acggctatag ccgcgctgaa ttcgtgaaac tgaccatccg caagctccag
     2041 gccttcgaat ccgaaccgcc gtgggccggc gatcgctcaa gcgacgaaca ggcggcccgt
     2101 acctggaagc atgtgcgggc cgacggaacg ctgatcgatc tggcgatcta ttcgcgccgt
     2161 ctgctctatg gcgaccggcc cgcggtattg ctggcgctga tggatattac cgagcgcaaa
     2221 cgcgccgagg cgcggctcgc cttcatggcg cagcacgacg gcttgaccgg gctgccgaac
     2281 cgcaatttgc tgcgccagcg catggacgag atcctgcaac acacccggcg cagcgcggac
     2341 aaggtcgcgg tgctggtgct cggtctcgat catttcaagg cggtcaacga tacgctcggc
     2401 cacggcgccg gcgacaagct gttgcgcgcc gtcgccaagc gcctgcgatc gacgttgcgc
     2461 gaggaagatg cgcttgcccg tctcaactcc gatgaattcg cgatcgtcca gagcgggttg
     2521 gcgcgaccgg aagatgcggt gttgctgtgc aagcggttac tcgaagcgat cggcgatccc
     2581 tttctgcttg acggtcattc cgtcgtggtt ggcgccagca tcggcatcgc gatggcgccg
     2641 agcgatggcg acgagtccga gaagctgctg aagaacgccg acatggcgct gtcccgcgcc
     2701 aagaacgatt cccgcggcac cttcagcttt ttcgaagccg gaatggacgc tcgcgcccag
     2761 agccgccgca agatcgagat cgatctgcgc gacgcgatcc agaatgacgt gctgcggccg
     2821 gattatcaac cgctgatcga cctttcgacc ggacgcatca ccggcttcga ggcgctgatg
     2881 cgctggccgc actccgaacg cggcatgata tcgccggccg aattcattcc ggtggccgaa
     2941 gagaccggcc tgatcaatgc gctcggcggc ctgatgctgc gccgcgcctg catggacgcg
     3001 gcactttggc ccgacgacgt ccgcgtcgcg gtcaacctgt cgccgctgca gttccgtgcc
     3061 ggcaatctgt tgtcggtggt gatggacgcg ctgaagcact ccggcctgcc ggcaaggcgg
     3121 ctggagctcg aaatcaccga aaccttgctg ttggaaaaga gcggccaggt actggccacg
     3181 ctgcacgcgc tgcgcgcgct cggcgtgcgc atctcgatgg acgatttcgg caccggctat
     3241 tccagcttga gctatcttcg aagctttccg ttcgacaaga tcaagatcga ccagtcgttc
     3301 gtgcgcgacc tcggcgccaa tcgcgacgcg caggcgatcg tgcgttcgat catcagcctc
     3361 ggcatcggtc tcggcgtgac cattaccgcc gaaggcgtcg agaccgaagc cgagttgagc
     3421 tgcctgcgcg ccgaggggtg ccacgagggc cagggctt
//
LOCUS       DASWGM010000180        10422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1397781,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000180 DASWGM010000000
VERSION     DASWGM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10422
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397781"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(545..2041)
                     /locus_tag="VGA15_05540"
     CDS             complement(545..2041)
                     /locus_tag="VGA15_05540"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX9587191.1"
                     /translation="MSDIILALRNATKLYAGVPAIENVDFELRRGEIHALVGENGAGK
                     STLTKVMAGVVTLTSGTMQVDGVEAAPRTPLEARHLGIAMVFQENSLVPTMTVAQNLF
                     LGQELFYNRLRGIYIAAQQFLQSLNFDVTPTATVGLLGAAKKQMVEIARAVLHEAKVI
                     IFDEPTATLTPEEKKYFFDLVRDLKRRGVSIVFISHALEEALILADRITILRDGKHVV
                     TDDARNFDRARIVQAMVGRDLSNTLYGTRKTTVRPAGVRVLTVQNLKMAPMVQNNSLS
                     VFAGQITGVFGLVGAGRTETFKVVAGILKRDFFHGGEVILHGKPVRYRVPAPAVRAGI
                     AYVTEDRKVEGFFETMSVARNIYLGLLAKLPGGRFFLSRREADKVGSTWVDKLKVRAT
                     GNDMKVVELSGGNQQKVVIAKSLVQDPDLIIFDEPTRGVDVGAIVEIHELIQRLADEG
                     KAVVVISSYLPEIMALSDRILVSRQGKVVEEFSAVEATEEKIMYAAVH"
     gene            complement(2038..3135)
                     /locus_tag="VGA15_05545"
     CDS             complement(2038..3135)
                     /locus_tag="VGA15_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587192.1"
                     /translation="MISALKDFVDRFLGRGDATITVPSFDGALKPNQILEKAETIGQF
                     EAPEDLATDGKALYIADGAAIVRLDGPTFTEIRRFDRAISALCCLPDGGIAAALDGRE
                     VQVFATPWAPSPRATITDPSMNAINALSPGAQGTLIATDGSTARPYGQWVYDLMERGR
                     TGRVLSLDIASGRVRPIASGLSYAFGACTAGDAVFVSESWRHRIIAIAQDGSQRAVLG
                     NLPVYPSRLSPAASGGFWLTAFAARTQLVEFVLRENAYRRRMMTEIDPEYWIAPKLKS
                     GQSFLEPMQGAHIKTMGVVKPWAPPRSYGLVIRLNADGLPLYAAHSRVDGVNHGVVAA
                     VEMEGSLYAIAKGPGRLLRAPLAMIERELRA"
     gene            complement(3132..5246)
                     /locus_tag="VGA15_05550"
     CDS             complement(3132..5246)
                     /locus_tag="VGA15_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9587193.1"
                     /translation="MQDALLRLRYRYWPDHLLGEILSKRWTETAIPVIVLLIITILLS
                     RSIPGFLSVGGLADTGRQAGEIGFVVLGMALVVIVGGIDLSVGSMFALCDFCALFCLN
                     VLDWPVPAVIVATLLCGVALGAINGLLIGYLRLRAFITTLITLIIYRSAYDLLLFDYS
                     NKIAAAFPDIPSWNFLGGGGVAGVPSVVLVYIVIAVFGHIFLTRLRPGWHVTAIGGSR
                     RSAYNSGIPVRRTIALCYTASGILTAIGALFFAARLGTVGGDVGVGLEVTVLTATVLG
                     GISLGGGKGSVAKALVGTLIVLFVINGLTTLSVSGGVNRMVLAGVLLVAATIDIRWLK
                     NRTRIISKVYVSPTFHGLGAAVSTEPGSGPFAQNDRLRDVTLIGLGRIEAPEDVILDR
                     NDNLYAGSRHGDIIRFLAPDYQQMEVFAHIGGQPLGMAFDRADNLYVCIGGMGLYRIT
                     PDRKVEKVTDETNRSLYSVNDDSRLRLADDLDIADDGRIFFSEATVRYEMHEWPVDGL
                     EARGNGRIICYDPNNRTTRTVLRGLKFPNGICVASDGQSILFAETFGCSIKRYWFDGP
                     NTGKIETVLENLPGYPDNINLASDGNYWLAMVGMRSPALDLAWKMPAFRKRMGKRVPI
                     DEWLFPNINTGCVIKFNERGEVLDSLWDLRGVNHPMITSMREHRGYLYLGGIANNRIG
                     RYKLAGADPDFVQYERRWRKTT"
     gene            complement(5357..6379)
                     /locus_tag="VGA15_05555"
     CDS             complement(5357..6379)
                     /locus_tag="VGA15_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9587194.1"
                     /translation="MALSGRTTLKTMLFGIGLIAAMASPALAQKGLDEPFQGAFKESL
                     AGKTVAYVPVAMNFDLTEGWFAGVKKELEPYGVKVIVRDPNWSTNAGAQAVTTLISEK
                     PAAMIIHNPDVQTYAKLLQRAENEGIYVIQINMGSSYRSSAFVGANWIEIGERDTEAV
                     VNACKGKSNKIAIIQGALSAAASAYTLKGVENVLAKNPDIKVVSSQAADWDAAKAKAI
                     TQTVLKQNPDLCGIVGFWDGMDIGTAAAVKEAGLTGKVFVATSGGGERRGACELVKSG
                     AFDLNLSYDVPTQAEQMAGVIKWLVSSGVKPGSVKGSMYTTLIPITKENAASDTACWN
                     LSDLKK"
     gene            complement(6420..7391)
                     /locus_tag="VGA15_05560"
     CDS             complement(6420..7391)
                     /locus_tag="VGA15_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9587195.1"
                     /translation="MGIRSFGQQGIVFAVFAALFAVFAVTLPGFFTTANMLTLLQNVA
                     VLGILGLAMAIVVIGRGIDISLIAALAVPPGLVLQMVQDGHSLPISLAAAFALTAVFG
                     LVNGWLIAYAEVPSLFTTLASGLFLAGLGQAALFKLDVVQWSPGLDGFERLGQGNLLG
                     IPMSIIMFALSCIAVAFLLRQTRIGAYIYAIGDNPNGARVTGIPTRPIIVLQYVLAAL
                     IGCVAGLILAASVNSMPTRIFNSTLIYDVILVVVLGGIGLSGGRGGVINVVIGTLLIG
                     TMLNGMTIMDVSYSGQNLVKGVVLLVAVIADSFLNPRNEETAQQGDI"
     gene            complement(7568..9640)
                     /locus_tag="VGA15_05565"
     CDS             complement(7568..9640)
                     /locus_tag="VGA15_05565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX9587196.1"
                     /translation="MPATRYLDTLNVQQRLAVEHGVRERDCAPRAPLLVIAGAGSGKT
                     NTLAHRVAHLIVNGADPRRILMMTFSRRAASEMTRRVERIARKVMGDGACVMTDALTW
                     AGTFHGIGARILRDYGDQIGLDPAFTIHDREDSADLINLVRHELGFSKTQNRFPTKGT
                     CLAIYSRCVNAESGIEQVLGKSFPWCSGWAAELKQLFAAYVEAKQRQNILDYDDLLLY
                     WAQTVSDPKLAEDIGGHFDHVLVDEYQDTNRLQSSILLALKPDGRGLTVVGDDAQSIY
                     SFRAATVRNILDFPGQFNPPATIVTLDRNYRSTATILAAANGVIELASERFTKNLWTE
                     RTSATKPQLVSVRDETDQARYIVERILESRESGTVLKQQAVLFRSSHHSGPLEVELTR
                     RNIPFVKFGGLKFLDAAHIKDMLAMLRFVENPRDRVAGFRLMHLIPGVGSTSAQRVLD
                     HMAKAADPIVALVEAPSPPRAGDDWQDFVGTVTDLGSGRAGWPAELEHARLWYEPHLD
                     RIHEDAGTRRADLVQLAQIAGGYPSRERFLTELTLDPPDATSDQAGVPLLDEDYLILS
                     TIHSAKGQEWKSVFVLNVVDGCMPSDLGAGTSAEIEEERRLLYVAMTRAKDDLHLVVP
                     QRFFTHGQGAQGDRHVYASRTRFIPERLLGLFEKTSWPLAATGTAARAASQGVRLDIG
                     ARMRGMWR"
     gene            complement(9737..>10422)
                     /locus_tag="VGA15_05570"
     CDS             complement(9737..>10422)
                     /locus_tag="VGA15_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX9587197.1"
                     /translation="VKDGDTVQLAGVTFKLDGIDAPEFDQMCIDEHAEPWACGVEARD
                     QLAKLIDKRGVHCEDLGPSKTYSKWHIGLCTVDGETTSLNQMLVRRGFALNFEPDAKG
                     RFKEDEANAKDKLSGLWRGCFVAPQDFRHDEKSGTLLGSACRSDKDRELREALFPDEP
                     AMPPGCSIKAKFARRARLTGNIGVYHLQACRSYAPVTKPDRWFCSEEDAQAAGFRKAY
                     NCRAIVRRK"
BASE COUNT         1984 a         3214 c         3246 g         1978 t
ORIGIN      
        1 catcgggggc ggcgccttgg tgtaaggacg tgccgccaga tcagccgccg acgcgggagc
       61 ggccatacct aacgctacca gaccaaccgt ggccaacaaa aatttcttca tcgtatttct
      121 ccatccccgt cgcccgcagt ggtctatctg aaacgataaa tgcactcgcg accgccgata
      181 actttgttgc agacactcgc ttgattccaa ttccatccac aacttatacg attgagtccc
      241 gaggttgcgt atctttaatg caacaacccg tttttcaaat atgactggaa aatggcaatc
      301 tgcatctgat cgcagagggt caggtctgct gcccttccaa agcaggacgc ctgccaattg
      361 agcccgcaca aattaatcct gcgcgttcat gagatcgtcg actcttgatg ctgtttgctg
      421 cgggtaagtg agtccggtat tgggacatcg cgccgtttcg ctgagatgca acggtttcgt
      481 ccgctaacgg ggacaaagcg gactcgtgcc gtcggctcga tcggcgcatc cacgatttca
      541 cggcctagtg aaccgccgcg tacatgattt tctcctcggt cgcttccacg gcggaaaact
      601 cctcgaccac tttgccttgc cgcgagacca gaatgcgatc ggacagcgcc atgatctcgg
      661 gcagatagga cgagatcacc accaccgcct tgccttcgtc ggccagtcgc tggatcagct
      721 cgtggatctc gacgatcgcg ccgacatcga cgccgcgcgt cggctcatcg aagatgatga
      781 gatccggatc ctgcaccagc gatttggcga tcacgacctt ctgctggttg ccgcccgaca
      841 attcgacgac cttcatgtcg ttgccggtgg cacgcacctt gagcttgtcg acccaggtgc
      901 tgccgacctt gtcggcctcg cgccgcgaca ggaagaagcg gccgccgggg agctttgcca
      961 gcagtccgag atagatgttg cgggcgaccg acatggtctc gaaaaagccc tcgaccttgc
     1021 ggtcctcggt gacgtaggcg atgccggccc gcaccgcggg cgcgggaacg cggtagcgca
     1081 ccggcttgcc gtgcaggatc acctcgccgc cgtgaaagaa atcgcgcttc aaaatgccgg
     1141 cgaccacctt gaatgtctcg gtgcggccgg cgccgaccag gccgaacacg ccggtgatct
     1201 ggccggcgaa caccgagagc gaattgttct ggaccatcgg cgccatcttg aggttctgca
     1261 cggtcagcac ccgcacgccg gcaggccgca cggtggtctt gcgcgtgcca tagagcgtgt
     1321 tcgacaaatc gcgtccgacc atcgcctgca cgatccgcgc gcggtcgaaa ttcctggcat
     1381 cgtcggtcac gacatgcttg ccgtcgcgca ggatcgtaat tcgatccgcc aggatcagcg
     1441 cttcctcgag cgcgtgcgaa atgaacacga tcgacacgcc gcggcgcttc aagtcgcgca
     1501 cgagatcgaa gaaatacttt ttttcctctg gcgtcagcgt ggcggtgggc tcatcgaaga
     1561 tgatcacctt ggcctcgtgc aacaccgcac gggctatttc gaccatctgc ttcttcgcgg
     1621 cgccgagcag tccgaccgtc gcggtcgggg tgacgtcgaa gttgagcgat tgcaggaact
     1681 gctgggcggc gatgtaaatt ccgcgcagcc ggttgtagaa aagctcctgt cccaggaaca
     1741 ggttctgcgc caccgtcatg gttggaacca ggctgttttc ctgaaacacc atcgcgatgc
     1801 cgagatggcg cgcttcgagc ggcgttcggg gggcagcttc tacgccgtcg acctgcatgg
     1861 tgccggacgt cagcgtcacc acgcccgcca tcacttttgt caatgtcgat ttgccggcgc
     1921 cgttctcgcc gacgagggcg tgaatctcgc cgcgacgcag ctcgaaatcg acattctcga
     1981 tcgcgggcac gccggcatag agctttgtcg cgttgcgaag cgcgaggatg atgtcgctca
     2041 tgcccgcaat tcccgctcga tcatggcgag cggcgcgcgc aacaaccggc ccggcccctt
     2101 ggcgatggcg tacagcgacc cctccatttc gacggccgcc accacgccat ggttgacgcc
     2161 gtcgactctg ctgtgagctg cgtaaagcgg caatccatcg gcgttgagcc tgatgacgag
     2221 gccataggat cgcggcggcg cccacggctt gacgacgccc atggtcttga tatgggcacc
     2281 ctgcatcggt tcgagaaacg actggccgga tttcagtttc ggcgcgatcc agtattccgg
     2341 atcgatttcc gtcatcatgc ggcgccggta ggcattttcg cgcagcacga attcgacgag
     2401 ctgggtgcgc gccgcgaatg ccgtgagcca gaagccgccg gacgccgccg gggaaagccg
     2461 cgacggatag accggcagat tgcccagcac ggcgcgctgg ctgccatcct gggcgatggc
     2521 aataatccga tgtcgccagc tttcgctgac gaagacggcg tcgcctgcgg tgcaggcgcc
     2581 gaatgcgtaa gacagccccg aggcgatcgg gcgcacccgt ccgcttgcga tgtcgaggct
     2641 gaggactctt ccggtgcggc cgcgctccat caggtcgtac acccattgcc catagggtcg
     2701 cgccgtcgag ccatccgtcg cgatcagcgt tccctgcgcg cccggcgaaa gcgcgttgat
     2761 cgcgttcatc gatgggtcgg tgatggtcgc ccgggggctg ggtgcccatg gtgtcgcgaa
     2821 cacctgcacc tcgcgcccgt caagtgctgc cgcgatgccg ccatccggca ggcagcacag
     2881 cgccgatatg gcgcggtcaa aacgtctgat ctccgtgaag gtcgggccgt cgaggcgcac
     2941 aatggcggcg ccatccgcga tgtagagcgc cttgccgtcg gtcgcgaggt cctcgggcgc
     3001 ttcgaactgc ccgatcgtct cggccttttc cagaatctgg ttgggtttca gtgcgccgtc
     3061 gaaggagggc accgtgatgg tggcatcgcc ccgtcccaga aagcgatcga caaaatcttt
     3121 caatgcggag atcatgtcgt ttttctccag cgccgttcgt actggacgaa gtcgggatcg
     3181 gcaccggcga gcttgtatcg gccgatacga ttgttggcga tgccgccgag atagagatag
     3241 ccgcgatgtt cgcgcatcga tgtgatcatc ggatggttga cgccgcggag atcccacagc
     3301 gaatcgagca cttcgccgcg ctcgttgaac ttgatgacgc atccggtgtt gatgttcggg
     3361 aacagccatt cgtcgatcgg aacccgcttg cccatgcgct tgcggaaggc gggcatcttc
     3421 catgcaagat cgagcgcggg gctgcgcatg ccgaccatcg ccagccagta gtttccgtcg
     3481 gacgccagat tgatattgtc gggatagccc ggcaggttct ccagcacggt ctcgattttc
     3541 ccggtgttcg ggccgtcgaa ccaatagcgc ttgatcgagc aaccgaacgt ttcggcaaac
     3601 aggatcgatt ggccgtcgct cgcaacgcaa atgccgttgg gaaactttag gccgcgcagc
     3661 acggtgcgcg tggttcggtt gttgggatcg tagcaaatga tccggccatt gccgcgcgcc
     3721 tccaggccgt cgaccggcca ttcgtgcatt tcgtagcgga cggtggcttc ggagaagaaa
     3781 atgcgcccgt catcggcgat atcgagatca tcggcaagcc gcagccggct gtcgtcgtta
     3841 acagaataaa ggctgcggtt ggtctcatcg gtcaccttct cgacctttcg atcgggcgtg
     3901 atgcggtaga ggcccatgcc gccgatgcac acgtagagat tatcggcccg gtcgaaggcc
     3961 atgccgagcg gttggccgcc gatatgagcg aacacttcca tctgctgata gtcgggcgcc
     4021 aggaagcgga tgatgtcgcc gtggcgcgaa cctgcataga gattgtcgtt gcgatcgagg
     4081 atgacgtctt ccggcgcctc gatgcggccg aggccgatca gcgtcacgtc acgcagtctg
     4141 tcgttctgcg cgaacggccc gctgccgggt tcggtcgaaa ccgccgcgcc caggccatga
     4201 aaggtcgggc tgacatagac cttgctgatg atgcgcgtgc ggttcttcag ccagcgaata
     4261 tcgatagtgg cggcgaccag cagcacgccg gcgagcacca tgcggttgac gccgccgctc
     4321 accgacagcg tggtcaggcc gttgatcacg aacaagacga tcagcgtgcc gaccagcgct
     4381 ttggccaccg agcccttgcc gccgccaagg ctgatgccgc ccagtacagt cgcggtcagg
     4441 accgtcactt cgaggccgac gccgacgtcg ccgccgacgg tgccgagtct tgctgcgaaa
     4501 aacagcgcgc cgatcgcggt caggattccg cttgcggtgt agcacaaggc gatcgtgcgc
     4561 cgcaccggaa tgcctgaatt gtaagccgag cggcgcgagc cgccgatcgc ggtgacatgc
     4621 catcccggcc gcaagcgggt gaggaagata tgtccgaaca cggcgatgac gatatagacc
     4681 agcacgacgc tcggcacgcc cgcgaccccg ccgccgccca aaaaattcca ggaggggatg
     4741 tcggggaacg cggcggcgat cttgttcgaa tagtcgaaca gcagcaggtc ataggcggat
     4801 cgatagatga ttagcgtgat cagcgtggtg atgaaggcgc gcagccgcag gtatccgatc
     4861 aacaggccgt tgatggcgcc aagcgccacg ccgcagagaa gcgtcgccac gatgaccgcc
     4921 ggcaccggcc agtccagcac attgaggcag aacagcgcgc agaagtcgca cagcgcgaac
     4981 atcgatccga ccgacaggtc gatgccgccg acgatgacga ccagcgccat gccgagcacg
     5041 acaaaaccga tctcgccggc ctgccgtccg gtatcggcaa gaccgccgac ggagagaaaa
     5101 ccggggatcg aacggctgag aaggatggtg atgatcagga gaacgatgac cgggatcgcg
     5161 gtttcggtcc aacgcttgga aagaatttcg ccgagcagat ggtctggcca gtaacggtat
     5221 cgcaaacgaa gaagtgcgtc ttgcatcggc ggcctggagg ggacggagga attttaatgg
     5281 atgcggtcgg cgcgctcgct tttgcgacga gcggccacgc gatggcggcc gctcgtccaa
     5341 atagcgcgga gctgatctat ttcttgagat cgctgagatt ccagcaggcg gtatcgctcg
     5401 ctgcgttctc cttggtgatc gggatcaggg ttgtgtacat cgatcccttc accgatcctg
     5461 gcttgacgcc ggatgagacc agccatttga tcacgccggc catttgctcg gcctgtgtcg
     5521 gcacgtcgta gctcaagttg agatcgaacg cgccggactt gacgagttcg cacgcgcccc
     5581 gccgctcgcc gccgccggag gtcgcgacga agaccttgcc ggtaaggccg gcttccttca
     5641 ccgcggccgc ggttccgata tccatgccgt cccagaagcc gacgatgccg cacagatcgg
     5701 gattctgttt caacacggtc tgcgtgatcg ccttggcctt ggcggcatcc caatcggcgg
     5761 cctggctcga gaccaccttg atatcgggat ttttggccag cacgttttca acgcctttca
     5821 gcgtgtaggc gctcgccgcg gccgacaacg cgccctggat gatggcgatc ttgttcgact
     5881 tgcccttgca ggcgtttacc accgcctcgg tgtcgcgctc gccgatctcg atccagttgg
     5941 cgccgacgaa ggccgaactg cgatagctcg atcccatatt gatctggatg acatagatgc
     6001 cttcattctc ggcacgctgc agcagttttg cgtaggtctg cacgtcggga ttgtggatga
     6061 tcatcgccgc cggcttttcg gagatcagcg tcgtcaccgc ctgtgcgccg gcgttggtgc
     6121 tccagttcgg atcgcggacg atcaccttga cgccgtaggg ctccagctcc ttcttgacgc
     6181 ccgcgaacca gccctcggtc agatcgaaat tcatggcgac cggcacgtaa gcgacggtct
     6241 tgccggcgag cgattccttg aaggccccct gaaaaggttc gtcgaggccc ttttgtgcga
     6301 gtgccggcga tgccattgcg gcgatcaaac cgatcccgaa caacatcgtc ttgagtgtcg
     6361 tccgtccaga taaagccata tcgtcctccc gttattgata ttgttgatga cgtagccgtt
     6421 cagatgtcgc cttgttgcgc ggtctcctcg ttgcgcgggt tcaggaaaga gtccgcgatc
     6481 accgcgacca gaagcaccac gcctttgacg aggttctggc cggaatagga gacgtccatg
     6541 atggtcatgc cgttgagcat ggtgccgatc agcagcgttc cgatgacgac attgatgacg
     6601 ccgccgcgcc cgcccgacaa tccgatgccg cccagcacga ccaccagaat cacatcgtaa
     6661 atcagcgtcg aattgaagat gcgcgtcggc atgctgttga cggaagctgc caggatcagg
     6721 ccagccacgc agccgatcag cgccgccagc acatattgca gcacgatgat gggccgggtc
     6781 ggaatgcccg tgacccgcgc gccattggga ttgtcgccga tcgcatagat gtaggcgccg
     6841 atccgggtct gtcgcagcag gaaggccacc gcgatgcacg acagcgcgaa catgatgatc
     6901 gacatcggta tgcccaagag attgccctgt ccgagccgct cgaaaccgtc gagccccgga
     6961 ctccactgca cgacgtcgag cttgaacaat gcggcctggc cgaggccggc gagaaacaat
     7021 ccggacgcca gggtcgtgaa cagcgacggc acctcggcat aggcgatcag ccatccgttg
     7081 acgagaccga acaccgccgt caatgcaaac gccgcggcga gcgagatcgg cagcgagtga
     7141 ccgtcctgga ccatctgaag caccagcccc ggcggcaccg ccaaggctgc aatcagcgaa
     7201 atgtcgatgc cgcgcccgat cacgacaatc gccatcgcca ggccgaggat gcccagcacc
     7261 gccacgttct gcagcagcgt caacatattc gcggtggtaa aaaagccggg cagggtaacc
     7321 gcgaagacgg caaaaagcgc ggcaaaaacc gcgaacacga ttccctgctg cccgaaactt
     7381 ctgattccca tcccggcttc tcttgtttgt tctattgccg gagatgttcg cgcgctttga
     7441 tcgccaatgc aagcgatcac ggtgatgcgc gcgatgacgc atcatcgaaa accgcaagcg
     7501 cccgcgccca aaaaatattc cgcgtaaaga aaagccgcgc agcgccacaa tcagagggtc
     7561 ttgaggctca ccgccacatc ccgcgcatac gggcgccgat gtccaagcga actccctggc
     7621 tcgcagcgcg ggcggcagtt ccggttgctg caagcggcca gcttgtcttt tcgaacaggc
     7681 cgagcagccg ttccggaatg aaacgcgtcc gtgacgcata gacgtggcga tcgccttgcg
     7741 cgccctggcc gtgggtgaag aaacgctgcg gcacgacgag gtgcagatcg tccttggcgc
     7801 gggtcatcgc cacatacaga agccggcgct cttcctcgat ttccgctgag gtgccggcgc
     7861 cgagatccga tggcatgcat ccatcgacga cgttgaggac gaacaccgat ttccattcct
     7921 ggcctttggc ggaatggatg gtggagagga tcagataatc ctcgtcgagc agcggcacgc
     7981 cggcctggtc gctggtcgca tcgggagggt cgagcgtgag ttcggtcagg aaacgctcgc
     8041 gcgaaggata gccgccggcg atctgcgcga gctggacgag atcggcgcgc cttgtgccgg
     8101 cgtcctcgtg aatgcgatcg agatgcggct cgtaccagag ccgcgcatgt tccagctcgg
     8161 ccggccagcc ggcgcggccc gagccgagat cggtgacggt cccgacgaag tcttgccagt
     8221 cgtcgccggc gcggggaggc gaaggggctt cgacgagagc aacaatcgga tcggcggcct
     8281 tggccatgtg gtcgaggacg cgctgcgccg atgtggaccc gacgccggga ataagatgca
     8341 tcagccgaaa tccggcgaca cggtcgcgcg ggttctcgac gaagcggagc atcgccagca
     8401 tgtccttgat atgcgcggca tcgagaaatt ttaagccgcc gaacttgacg aacgggatgt
     8461 tgcgccgggt cagttcgacc tccagcgggc cgctgtgatg cgagctgcgg aacagcaccg
     8521 cctgttgctt gaggacggtc ccggattcac ggctctcaag gattcgttcg acgatgtagc
     8581 gggcctggtc ggtctcatcg cgcaccgaca ccagctgcgg ttttgtggcg gaggtccgct
     8641 cggtccacag gttcttcgta aaacgttcgc tggcgagttc gatgacgcca ttggcggcgg
     8701 cgagaatggt cgctgtcgaa cggtagttgc gatccagcgt cacgatcgtg gcaggcggat
     8761 tgaattggcc ggggaagtcg agaatgttgc gcaccgttgc cgcacgaaac gaatagatcg
     8821 actgcgcgtc atcgccgacc acggtaagac ctcggccgtc cggcttcaga gccaacagga
     8881 tcgaggactg caggcggttg gtgtcctggt attcgtcgac caggacatgg tcgaagtggc
     8941 cgccgatgtc ttctgccaat ttgggatcgc tgacggtctg cgcccagtac agcaggaggt
     9001 cgtcgtaatc gagaatgttt tgccgctgtt tggcttcgac ataggcggcg aacaattgct
     9061 tgagctcggc cgcccatccc gaacaccacg gaaacgattt tccgagtacc tgttcaatgc
     9121 cggactccgc attgacgcat cgggagtaga tcgcaaggca cgtgcccttg gtcggaaagc
     9181 ggttttgggt tttggagaag ccgagttcgt gccgcaccag attgatcagg tcggccgaat
     9241 cctcgcggtc atggatcgtg aacgccgggt cgagaccgat ctggtcgcca tagtcccgca
     9301 gaattctggc tccgatgccg tgaaacgtac ccgcccaggt cagcgcgtcg gtcatgacgc
     9361 aggctccgtc gcccatcacc ttacgcgcaa tccgctcgac gcggcgggtc atctcgctgg
     9421 ccgcacggcg cgagaatgtc atcatcagaa tgcggcgcgg atcggcgccg ttgacgatga
     9481 gatgggcgac ccgatgcgcc agcgtattgg tcttgccgga tcccgcgccc gcgatgacca
     9541 ggagcggtgc ccgcggcgcg caatcgcgct cccgcacgcc gtgctcgacc gcaagccgct
     9601 gctgcacgtt gagggtgtcg aggtatctcg tagcgggcat gattgattcg tccgaggaag
     9661 aacgacgctc gctgattcct gctttgttcg caatggccga ctggatcagg tgccggttcc
     9721 ccggcatgcg cggcgcttac ttgcgacgaa caattgcccg gcaattatac gccttgcgaa
     9781 accccgcggc ctgggcgtct tcctcggagc agaaccagcg atcgggtttt gtcactggtg
     9841 cgtaactccg gcacgcctgc aggtgataaa caccgatgtt acctgtcagg cgcgcgcgcc
     9901 tggcgaattt tgccttgatg ctgcagccgg gcggcatcgc cggttcgtcc ggaaacagcg
     9961 cctctcgcag ctcgcggtcc ttgtcgcttc ggcaggccga gcccaacaag gtgcctgact
    10021 tttcgtcatg gcggaaatcc tgcggggcga caaagcagcc tcgccagagg ccgctcagct
    10081 tgtcctttgc gttcgcctcg tcctccttga accggccctt ggcgtcgggc tcaaagttca
    10141 gcgcaaaacc ccgacgcacc agcatctggt tcagactggt ggtttcgccg tcgacggtgc
    10201 aaagcccgat atgccatttg ctgtaggttt tgctcggccc cagatcctcg cagtggacac
    10261 cgcgcttatc aatcagtttc gcaagctggt cgcgcgcttc cacgccgcac gcccagggtt
    10321 cggcatgttc gtcgatgcac atctggtcga actcaggagc atcgatgcca tcgagtttga
    10381 aagtcacgcc ggcgagctgg acggtgtctc cgtccttgac ga
//
LOCUS       DASWGM010000181         3758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_16042,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000181 DASWGM010000000
VERSION     DASWGM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16042"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            468..914
                     /locus_tag="VGA15_05575"
     CDS             468..914
                     /locus_tag="VGA15_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587198.1"
                     /translation="MIVRISLFRPFSRSAAALAVLALAAGLGGCADMSDNMSTAFADP
                     AKYDLYECKQLEPERKSLAARAAELQGLMAKAQTGVAGPVVAELAYRNDYIAVRGQAK
                     LAEEAWQRNKCVATPEKPAQAAAASTPPAKKSKRAKSPMLPGTAID"
     gene            complement(1053..2255)
                     /locus_tag="VGA15_05580"
     CDS             complement(1053..2255)
                     /locus_tag="VGA15_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9587199.1"
                     /translation="MAASRTIVVAGAGIGGLTAALALAGRGFRVVVLEKAERLEEAGA
                     GLQLSPNASRVLIDLGLQPRLAARAVTPEAISIMSAQRGGEVIRLPLGEAATSRAGAP
                     YWVMHRADLQAALQAQVNDHPDIELRLGCQFEDVVPHAKGLTVVQRSGMTRQQELAVA
                     LVGADGIWSTVRHHLFPEVQPQFSGLIAWRGTLDTTQLPREYASCRVQIWMGPDAHLV
                     AYPISGAQKINVVAVVPGTWNRPGWSAAGEVCEIKNAFASARWPGNARMIIGAVDDWR
                     KWALFTIPDGGEWTAGAIALLGDAAHAMLPFAAQGAGMAIEDAAVLARCLGERVGESA
                     AGIPAALKNYAQLRRSRVARVQRAARQSGRIYHLPGPAAYARDLAIKAMGARRVLARQ
                     DWIYDWRA"
     gene            complement(2263..2508)
                     /locus_tag="VGA15_05585"
     CDS             complement(2263..2508)
                     /locus_tag="VGA15_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX9587200.1"
                     /translation="MSDHVVPHFHNDAGVSVIEIGSQEFMCVGANPPFDHPHVFLDLG
                     NDNEIICPYCSTLYRYAADLKAGEARPPECVLKDKVA"
     gene            2878..>3758
                     /locus_tag="VGA15_05590"
     CDS             2878..>3758
                     /locus_tag="VGA15_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587201.1"
                     /translation="MKRTRIGSALVGTTVCAVACVLLWPHARDAGAILAAQDDPAALS
                     DIQLNSALRDNQPVVAANIEAALAANDADLANSFVELAREKDIPVADELSRRVSEAVT
                     EENSSSHFAKRFATGFVTGNADDVASLSGTVAGDLFVFGDIRDVVREGKHLAMGEDTD
                     RLVLGLATAGLAVTAVTYVSVGGAAPVRAGLTMVKDARKVGRLGEGLTQWAGRSAREV
                     VDAPMLQHAVASGSVMRPGETMTAIRAAFRAEKAGGLVRLAKDVGRVGEKAGTRSALE
                     TLRIAEGPKDVARAARLA"
BASE COUNT          677 a         1178 c         1219 g          684 t
ORIGIN      
        1 cggtgtcaga acgcatcggt ctttgaacaa ggatgcacca gtctctcgct cggtacagcg
       61 caccggtgtc attagttcac gcgccatcct gggcggactt catcaccact atggtcggat
      121 ttaaggtttt cggtacacac aggtcgcgga aaccagcccg gaaacggttt tccaaatcac
      181 gttttgtccc atcggtttct atcgtgattg gagggcgcgt tttccaaaaa aggtcaggac
      241 tttcaaagag ggcagagaat tttaagtccc ttgcgtctac cagttccgcc acgtccgctt
      301 tttgttctca gatcagatac ttagcgcgtt ttctggccaa gtggaatgag cctcgccggg
      361 agaacgcgct ccttctatag tggagcgcgt tgcttaaggc aaagcctcaa tgccgacacc
      421 tgccggtggt ttacgcaacc tcaatgttgt ccacttacga aggtcgcatg atcgttcgca
      481 tttccctttt ccggccattt tcgcggagcg ccgcggcgct ggcagtgctc gcgctcgccg
      541 ccggacttgg cggctgcgcc gatatgagcg acaacatgtc caccgccttc gccgatcccg
      601 ccaaatacga tctgtacgag tgcaagcaat tggagccgga gcgcaagagc ctcgccgccc
      661 gcgcggcgga gttgcagggc ctgatggcga aagcccagac cggggttgcc ggtcctgtcg
      721 tggccgaact cgcctatcgc aacgactaca tcgcggtgcg agggcaggcc aaactcgccg
      781 aggaagcctg gcaacgcaac aaatgcgtcg ccacgcctga aaagccggcg caggctgcgg
      841 ccgcgtcaac cccgcccgca aaaaagagca aacgtgcgaa atcgccgatg ctgccgggca
      901 ccgcgatcga ttagatcatg atccgattga gccggatcat gatctccggg caaacgcttc
      961 gcgtttgtcc cgagggaaaa caggtttcca ccctcgggtc acgcccgagg gcatgcttgc
     1021 ggatcatgtc ctaagcgggg gacaaggccg ccttaggccc gccagtcata gatccagtcc
     1081 tgtcgcgcca gcacgcgccg cgcgcccatc gccttgatcg cgaggtcgcg cgcgtaagcc
     1141 gccggacccg gaagatgata gattcgcccg gattgccgcg cggcgcgctg gacccgtgcc
     1201 acgcgcgagc ggcgcagctg cgcgtagttt tttagcgcgg ccgggatgcc ggcggcgctt
     1261 tccccaactc tttcaccaag gcatcgtgcc agcaccgccg catcctcgat cgccattccg
     1321 gcgccttggg cggcaaacgg cagcatggcg tgggcggcat cccccaacaa ggcgatggcg
     1381 ccagcggtcc attcgccgcc atcggggatc gtgaacagcg cccatttccg ccagtcgtcg
     1441 acagcaccaa tgatcatccg cgcgttgccc ggccaccgcg ccgatgcgaa cgcgttcttg
     1501 atctcgcaga cttcgcccgc cgcgctccag cccggcctgt tccaggttcc cggcacgacg
     1561 gcgacgacat tgatcttttg tgcgccggat atcggataag cgaccagatg cgcgtccggt
     1621 cccatccaga tctggacccg gcaggaagca tattcgcgcg gcaattgcgt ggtatcgagc
     1681 gttccgcgcc aggcgatcag cccggagaat tgcggttgca cctcgggaaa caggtgatgc
     1741 cggaccgtgg accagattcc gtcggcgccc accagcgcca ccgccagttc ctgttggcgc
     1801 gtcatgccgc tgcgctggac cacggtcagt cccttggcgt ggggcacgac gtcttcgaac
     1861 tggcagccga gccgcagctc gatatcggga tgatcgttga cctgagcctg cagcgccgcc
     1921 tgcaggtcgg cgcgatgcat cacccaatag ggcgcgccgg cgcgcgacgt ggcggcttcg
     1981 ccgagcggca ggcggatgac ctcaccgccg cgttgcgcgc tcatgatgct gatggcctcg
     2041 ggcgtaacgg cgcgcgcggc gagccggggc tgcaggccca ggtcgatcag cacgcggctg
     2101 gcgttgggtg aaagctgcag gccggcgccg gcttcctcga ggcgctcggc tttctcgaga
     2161 acgacgacgc gaaagccgcg tcccgccagc gccagcgccg ccgtcaatcc tccgatcccg
     2221 gcaccagcga cgacgatggt gcgtgaggcg gccaccggcc gctcaggcga ctttatcctt
     2281 cagcacgcat tcaggcggcc gggcctcgcc ggccttgagg tcggcggcgt aacgatacag
     2341 ggtggagcaa taggggcaga tgatctcgtt gtcgttgcca agatcgagga acacatgcgg
     2401 gtggtcgaac ggcggattgg cgcccacaca catgaattcc tgcgagccga tctcgatgac
     2461 cgaaacgccg gcatcgttgt gaaagtgcgg aacgacatga tcggacattg gattcaccgt
     2521 ggatagatgg aggcggacgc agggtcgaaa cgcagcgcac catactggcg ggtgccattg
     2581 attcctagtg tcccggatcc gcagttgcag cacccttttt gagtgaactt gcggatttga
     2641 aaggacgcta gatctctgat tctagtgtgg gcgtggatca gaagttctct gtggcgaacc
     2701 cgactggcac tgacggaggg aacttgtgag ccaccacagg gagccccaat cgctcattcg
     2761 ctcatgcaaa ttcgacacaa tcttgtcgtc ccagaaaagc ccttctttcg tcggggccgt
     2821 tgtgtcttaa aagcggcaca ccatctcgag ggaagcgaag acatcggggt ttttgggatg
     2881 aagcggacgc ggatcggatc ggctctggtt ggtaccacgg tgtgcgcggt ggcctgcgtg
     2941 ctgctgtggc cgcatgcgcg cgacgccggc gccattctgg cggcgcagga cgatcccgct
     3001 gcgctctccg acatccagct caattccgct ctgcgggaca accagcccgt cgtcgcagcg
     3061 aatatcgagg cggcacttgc cgcaaacgat gccgatcttg ccaacagctt tgtcgagctc
     3121 gcccgggaaa aggacattcc cgttgccgat gagttgtcgc gacgcgtatc cgaggccgtc
     3181 acggaagaaa attcgtcgtc gcattttgca aagcgcttcg ccaccgggtt tgtgaccggg
     3241 aacgccgacg atgtcgccag cctgtcgggc acggtcgccg gcgacctgtt cgtgttcggc
     3301 gacatcaggg acgtggtgcg cgaaggcaag catctggcga tgggtgagga caccgatcgg
     3361 ctggtgctgg gtctggccac ggcgggcctt gcggtgacgg cggtgaccta tgtctcggtc
     3421 ggtggcgcgg cgccggtgcg cgccggtctc actatggtca aggatgcccg caaggtcggg
     3481 cggctgggcg aggggctcac gcaatgggcc ggccgctccg cacgcgaggt ggttgacgcg
     3541 ccgatgctgc aacatgcggt cgcgtccggc tcggtgatgc ggccggggga gaccatgaca
     3601 gcgatcaggg cggcgtttcg cgcggagaag gccggcgggc tcgtccgtct tgccaaggac
     3661 gttggccgcg tcggtgagaa ggccggcacc cgcagcgccc tcgagacgct gagaatcgcc
     3721 gagggcccga aggatgtcgc tcgcgccgca cggctggc
//
LOCUS       DASWGM010000182         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_705734,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000182 DASWGM010000000
VERSION     DASWGM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705734"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..799
                     /locus_tag="VGA15_05595"
     CDS             38..799
                     /locus_tag="VGA15_05595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9587202.1"
                     /translation="MSGLPNSLHALVTGGGRGIGREIASTLSRAGATVTVLGRNRATL
                     DEAVALGCAHFASVADVADQAAVSGAIAAAAARQPIDILIANAGVAESAPFGRSDAAL
                     FKRMIDVNFMGVVHAVQAVLPSMRGRPYGRIVAIASTAGLKGYAYVSAYSAAKHAVVG
                     LVRSLALELATTGVTVNAVCPGFTDTDLLAGSIDNITRKTGRSHEQALAELAKHNPQG
                     HLVTPAQVADTVLWLCGEGAGAITGQAIAVAGGEI"
     gene            930..1766
                     /locus_tag="VGA15_05600"
     CDS             930..1766
                     /locus_tag="VGA15_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX9587203.1"
                     /translation="MSTPANPVTLPLAQYSPRHFLLAVNDGVATVTLNRPERKNPLTF
                     ESYRELTDFFRACAMDDDVKTVVVSGAGGNFSSGGDVFEIIGPLVKMDTKGLTAFTRM
                     TGDLVKAMRACPQPIVAAVDGICAGAGAIIAMASDIRLAATGAKVAFLFNKVGLAGCD
                     MGACAILPRIIGQSRASELLYTGRFMTAEEGERWGFFSRIVTPEHVLSQAQLLARQVS
                     EGPTFANTMTKRMLAMEWAMSVEEAIEAEAIAQALCMTTADFARAFEAFSNKVKPAFQ
                     GN"
     gene            1847..>3055
                     /locus_tag="VGA15_05605"
     CDS             1847..>3055
                     /locus_tag="VGA15_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9587204.1"
                     /translation="MKIAIIGGGPAGLYSAILLKKQRPEADITVYERNRADDTFGFGV
                     VFSDATLDNFEKYDAPSYRRITQEFAYWDDIAVHFRGTVHRVGGNGFCGCSRRTLLLI
                     LQERARELGVALLFECDIDDEAHFADADLVVLADGINSRFRDKYIEHFQPEVDLRSNM
                     FAWMGSTRPLDAFTFIFQETEWGPFIAHAYQYEVGRSTWIFETDPATFKRAGLEGLSE
                     KQSADRMAGIFGWFLEGHQLLINRSMWRNFPMIRSKRWVKDNMVLLGDAKATAHFSIG
                     SGTKLAMEDAIALADAMHRAPTAKAALRQYEEGRREEVEKTQHAADVSLVWFEHVDRF
                     WNFDPVQFAFGVMTRAKAITYENLTLRAPDFVAEVDKAFARQVRAKGFDVDVEKPVAP
                     MFQPLRLREME"
BASE COUNT          585 a          888 c          972 g          610 t
ORIGIN      
        1 ttgtcacatt gacgaattca ttcggggagg cccgcgcatg tccggactgc cgaattccct
       61 gcatgcgctc gtcaccggtg ggggacgagg catcggccgc gagatcgcgt ccacgctttc
      121 gcgcgcggga gcaaccgtca ccgtgcttgg ccgtaaccgc gcgacactgg acgaagcggt
      181 tgccctgggt tgcgcgcact ttgcaagtgt cgccgatgtc gcggatcagg ctgcggtgag
      241 tggcgcgatt gcggcggctg cggcgcggca gccgatcgat atcctgattg caaatgcggg
      301 ggtcgcggag tccgcgccgt tcggaagatc cgatgcggcg ctattcaagc gaatgatcga
      361 cgtaaacttc atgggcgtgg ttcacgccgt ccaagccgta ttaccttcga tgagaggccg
      421 tccatacggc cggatagttg ccatcgcatc caccgcgggt ctcaaaggtt acgcctatgt
      481 cagcgcctat agcgccgcca aacacgccgt ggttggtttg gtgcgatcat tggcgctgga
      541 gttggcgacc acgggcgtga cggtgaatgc agtttgtccc ggcttcacag ataccgatct
      601 gcttgccggc agtatcgaca acatcacgag aaagactggc cgcagccatg agcaggcctt
      661 agccgaactc gcgaagcaca atccgcaggg ccacctcgtg acccccgcgc aggtcgccga
      721 taccgtgctt tggctgtgcg gcgagggggc cggtgccatc acgggacagg ccatcgcggt
      781 tgcaggcgga gagatttgag ccgcgtgcgt cgcgcgcaag tctaaaccgt cattgcgagc
      841 gcagcgaaga ttccagaaca agaaacgata gttctggatt gcttcgtcgc ttcgctcctc
      901 gcaatgacaa cgatatcaag ggagatctta tgagcacgcc agccaatccc gtcacgttgc
      961 cattggcgca gtattcgccc cgtcatttcc tgctggcagt aaatgatggc gtggcaacag
     1021 tgacgctgaa ccgccccgag cgaaagaatc cgctgacatt cgaaagctac cgcgaattga
     1081 ccgatttttt ccgcgcctgc gcgatggacg atgacgtaaa gaccgtcgtg gtatcaggtg
     1141 ccggcggcaa cttttcgtcg ggcggcgatg tgttcgagat catcggcccg ctggtcaaga
     1201 tggataccaa gggcctcacc gccttcaccc ggatgaccgg cgacctcgtc aaggcgatgc
     1261 gggcctgccc acagccgatc gtagctgccg tcgacggcat ctgcgccgga gcgggcgcga
     1321 tcatcgccat ggcgtccgat atccgcctgg cggctacggg agccaaggtc gcgttcctgt
     1381 tcaacaaggt cggccttgcc ggctgcgaca tgggcgcctg tgcgatcctg ccgcgcatca
     1441 tcgggcaatc gcgggcgtcg gagctgctct ataccggtcg cttcatgacc gcggaggagg
     1501 gcgagcgctg ggggttcttc agccgtatcg ttacgccgga gcatgtgttg tcgcaggcgc
     1561 agcttctggc ccggcaggtt tccgaagggc cgacattcgc caataccatg accaagcgca
     1621 tgctggcgat ggaatgggca atgtcggtgg aagaggcgat cgaggccgag gcgatcgcgc
     1681 aggcgctgtg catgaccaca gcggattttg cccgcgcatt cgaggcattt tcgaataaag
     1741 tgaagccggc atttcagggc aattgagcat ccggttgcgg gcttgtcgga aaaatgcttt
     1801 aggtttaaaa taattgaacg atccgacaga agcgccggag gctctcatga agatcgcgat
     1861 catcgggggt ggaccggccg gtctctattc cgcgatcctg ctcaaaaaac agcggcccga
     1921 agccgacatt acggtctacg agcgcaaccg cgccgacgac acttttgggt tcggcgtggt
     1981 gttctccgac gcgacgctgg acaatttcga gaaatacgat gctcccagct atcgccgtat
     2041 cacccaggag ttcgcctatt gggacgatat cgccgtgcac ttccgcggca cggtgcaccg
     2101 ggtcggcggc aacggttttt gcggttgttc gcgtcgcacg ctgctcctga tcctgcagga
     2161 gcgggcgcgc gaactcggcg tcgccttatt gttcgaatgc gatatcgacg acgaggccca
     2221 ttttgccgat gcggatctgg tggtgctggc cgatggcatc aacagccgct tccgcgacaa
     2281 atacatcgag cacttccagc ccgaggtgga tctgcgctcc aacatgttcg cctggatggg
     2341 ttcgacccgg ccgcttgatg ccttcacgtt tatttttcag gagaccgaat ggggtccgtt
     2401 cattgcgcat gcctaccaat acgaagtcgg gcgttcgacc tggatctttg agaccgaccc
     2461 cgcaaccttc aagcgggccg gacttgaggg cctcagcgag aagcaatccg ccgatcgaat
     2521 ggctggcatt ttcggttggt tcctcgaagg gcatcagctg ctgatcaatc gctcgatgtg
     2581 gcgcaatttc ccgatgatcc gcagcaagcg ctgggtcaag gacaacatgg tgctgcttgg
     2641 ggatgccaag gcgactgcgc atttctcgat cggctccggc accaagcttg ccatggaaga
     2701 cgcgattgca ttggcggacg ccatgcatcg cgcgccgacg gccaaggccg ccttgcggca
     2761 atacgaggaa ggacgccgcg aggaggtcga gaagacccag cacgcggccg acgtgtcgct
     2821 ggtgtggttc gagcatgtcg accgtttctg gaacttcgat ccggtgcagt tcgcgttcgg
     2881 cgtcatgacc cgggcgaaag caatcactta cgaaaatctg acactgcgcg cgccggattt
     2941 cgttgccgag gtcgacaagg ccttcgcaag acaggttcgt gccaagggtt ttgacgtcga
     3001 cgtcgagaag ccggtggcgc cgatgtttca gccgttgcgg ctgcgcgaga tggag
//
LOCUS       DASWGM010000183         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_846827,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000183 DASWGM010000000
VERSION     DASWGM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846827"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1934)
                     /locus_tag="VGA15_05610"
     CDS             complement(150..1934)
                     /locus_tag="VGA15_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9587205.1"
                     /translation="MQSRAKHDPQTAATEAGGGFAVDTELSVLRDVFRLLPGGVTVQD
                     EQGRFLLVNDAAAAQLGIAVGGPAAPDSNELTHRRETGLELLRAGRCAVIEESVTGSE
                     GKRTLLTAHRPVRIADRTLLLSSSADISEQKAIEAQLFRSAYYDELTGLPARRVIERR
                     ANSLLQRDETQARFALAFLDIDNFKQINDYYGHAIGDALLVEVAKRIGLNLRESDMLS
                     RISGDEFLLLLDPIQGEHEVTEYVDFVLQRLRAPFFIDGSEIFASASIGVGLCPEHGR
                     SFDQLCQNADIAMDSVKNDGKGAAAFFDAGMERDALARMKVEQSLRLAILEKRFCCAF
                     QAKVDIRTQDIRGIEALVRLRDDDGVIQAPGTFVNLAVELGLIDELTHLVLAEIVKSI
                     DLINDTFGPDTSISINVAARQAGNPQFMRPFALALEATGYPKRFMIEVTEDAFVAKTH
                     FQDEILPMFRKLGVGVSIDDFGIGYSSLSALADITADEIKIDRSFITDIHKRPRSQGI
                     LRAIESLSEALGMTVVAEGIETFEELAYLQAATKIRYAQGYYFSRPIFLEELRPPIRV
                     LDHARASAASRPHQESRSVYARNERYRG"
     gene            complement(2085..3047)
                     /locus_tag="VGA15_05615"
     CDS             complement(2085..3047)
                     /locus_tag="VGA15_05615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9587206.1"
                     /translation="MVKLIEEFRRGWHGTSQPAPIFNVGFAATCLALSTAARWGFALI
                     RPDIFFTPYIPAVFFATALGGLRVGIATAIVGGTLGVTVSFSNAPADFTRMVLLAIYL
                     IVCGLTIWGIEHYRSIASQQREISRRLIREEEYRKLIVDEFQHRLKNKLSTIHAVLHL
                     VLQDQPQIWAGIDQRLRAMSATDDLIAKVDGSGCDIRDLLLSELGPYGHVRFTLNGNP
                     LFLPAKLAVSLALIFHELATNAGKYGAFSSARGLLQVSWSVSGDRLNITWDETEGPPV
                     ASVGEAGFGTKLLKAALTPFDGKSEISFLKTGVHCAMQCRIPLA"
     gene            3235..>3569
                     /locus_tag="VGA15_05620"
     CDS             3235..>3569
                     /locus_tag="VGA15_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9587207.1"
                     /translation="MGRLDGKVAVITGATSGIGLRTAEIFVAEGARIVIAGRRVPEGE
                     ALASKLGANCVFRRTDVTVEGEMRALIGQAVEKFGRLDCLFNNAGGPAQTGGIEGLEV
                     VRFDAAMATL"
BASE COUNT          740 a         1071 c         1095 g          663 t
ORIGIN      
        1 gcgagcgcgg catcatggat atcatcggca tcatcggcta ttacgatctg gtctcgatga
       61 cgctgatcac catgcaggcc gaggcgccga acgacagcgt gccgccactg caggctttat
      121 cgaaataaat gcgagcgtga acccgcgcgt cagccgcgat aacgctcgtt gcgcgcataa
      181 accgagcggc tttcctggtg cgggcggctc gccgcactgg cgcgcgcatg gtccaggacc
      241 cgaatcggcg gcctgagttc ttcgagaaaa atcggcctgg agaaatagta accctgcgcg
      301 tagcggatct tggtggcggc ctgcagatag gcgagttcct cgaaggtttc gatgccctct
      361 gcgacgacgg tcatgccgag ggcttcgctc aacgattcga tcgcccgcaa gatcccctgg
      421 ctgcgcgggc gcttatggat gtcggtgatg aacgaccggt cgattttgat ttcgtcggca
      481 gtgatgtcgg ccagcgccga caacgaggaa tagccaatgc cgaaatcgtc gatcgagacc
      541 ccaactccga gcttgcggaa catcggcaga atttcgtcct ggaaatgggt cttggcaacg
      601 aaggcgtctt cggtcacctc gatcatgaag cgcttggggt agccggtcgc ctcaagggct
      661 aacgcaaacg ggcgcatgaa ctgcggattg ccggcctgcc tggccgcgac gttgatgctg
      721 atgctggtgt cggggccgaa ggtgtcgttg atcaggtcga tcgatttgac gatttccgcc
      781 agcaccaggt gggtaagctc gtcgatcagt ccgagctcca cggcgagatt gacgaatgtg
      841 ccgggcgcct gaatgacccc gtcgtcatcg cgcagccgga ccagcgcctc gatgcccctg
      901 atatcctggg ttcggatgtc gactttcgcc tggaaggcgc agcaaaagcg cttttccaga
      961 atagcgagcc gcagcgactg ctcgaccttc attcgcgcaa gcgcatcgcg ctccatgccg
     1021 gcgtcgaaaa aggccgcggc gcccttgccg tcgttcttga cgctatccat cgcgatatcg
     1081 gcgttctggc acaactggtc gaagctgcgg ccgtgctcgg gacaaaggcc gaccccgatc
     1141 gaagcagatg cgaatatttc cgatccatcg atgaaaaacg gagcccgcag ccgctgcagc
     1201 acaaagtcga cgtattccgt cacctcgtgc tcgccctgga tcggatcaag cagcagcagg
     1261 aattcgtcgc cgctgatccg cgacagcatg tcggattcgc gcaaattcaa cccgatccgc
     1321 tttgcgactt ccaccagcag cgcgtccccg atcgcatgcc cgtaatagtc gttgatctgc
     1381 ttgaaattgt cgatgtcgag aaatgcgagg gcgaacctcg cctgcgtttc gtcgcgctgc
     1441 agaagactgt tggcgcgacg ttcaatcaca cggcgcgccg gcagaccggt cagctcgtca
     1501 tagtaagccg accggaacag ctgagcctcg atggcctttt gctcactgat atcggcagag
     1561 ctcgaaagca gcagagtgcg gtctgcaata cgaaccggcc gatgggccgt gagaagcgtc
     1621 cgtttgccct cactgccggt caccgattcc tcgataacgg cgcagcgacc ggcgcgcagc
     1681 aattccaggc cggtttcgcg gcggtgggtc agttcgttcg aatccggcgc tgccggcccg
     1741 ccgacggcaa tcccaagctg cgccgcggcg gcatcgttaa ccagcaggaa gcggccttgc
     1801 tcgtcctgaa ccgtcacgcc gccggggagc aatctgaaaa catcccgcag aacgctcagc
     1861 tcggtatcca cggcgaatcc gccaccggct tccgtcgcgg cggtctgcgg gtcgtgtttg
     1921 gcgcggcttt gcataaccgc gaagtctgcc aagctctctt gtagttttgg ttaagtggca
     1981 gcgacaaact cgatgagacg gcgggtagac agccgaggga tccaaacccc gcgaggcgtc
     2041 gttaacaacg tgtcaacggg tcttcctgcg tgcccaggac ccgttcacgc cagcggtatg
     2101 cggcactgca tcgcgcaatg aaccccggtt ttcaaaaaac tgatctcgct cttgccgtca
     2161 aaaggagtga gcgccgcttt cagcaacttg gtcccaaatc cggcctcccc gacacttgcg
     2221 accggaggac cctcggtctc gtcccaggta atgttgaggc gatcgcctga cacggaccac
     2281 gatacctgca atagcccgcg cgccgaagaa aacgcgccgt actttccggc gttggtggcg
     2341 agctcgtgaa agatcaatgc caggctcacg gccagtttgg cggggagaaa cagcggatta
     2401 ccgttcaggg taaatcgaac atgcccgtaa ggtccgagtt cggaaagcaa gagatccctg
     2461 atatcgcaac cgctgccgtc cacctttgcg atcagatcat cggtcgcgga catcgcccgt
     2521 aaccgctgat cgatgccggc ccagatttgc ggttgatcct gcagcactag atggagaacg
     2581 gcgtgaatgg tcgacaattt gtttttcagc cggtgctgga actcgtcgac aataagcttt
     2641 cgatattcct cctcccggat caggcgcctt gagatctccc gttgctgcga cgcgatcgat
     2701 cggtagtgct cgatgcccca tatcgtgagg ccgcagacga tcagatagat cgccagcagc
     2761 accattcttg taaaatcggc cggggcgttg ctgaaactga cggtcacgcc gagcgtgccg
     2821 ccgacgatgg ctgtcgcgat cccgacccgc aaaccaccga gtgcggtggc gaagaatacg
     2881 gctggaatat aaggagtgaa aaatatgtcc ggccggatca gggcaaagcc ccaccgcgcc
     2941 gcggtcgaca gagcgaggca ggtggccgca aatccaacgt taaaaatcgg cgctggctgg
     3001 gatgttccat gccagccgcg ccgaaactcc tcgatcaatt tcaccatgcg tcacaaagct
     3061 caagcgatca gcgaatcctg aaagggcgga gtataggaga aaatacgcgg cccgcggcca
     3121 caaaggcgac aacccggccc ggcatcaggg cacgcaaccg gcatgggctt gcgttgttcc
     3181 ggcggggtgg gaatatagcg gaacacaata ttattcaaaa aagaggagga tgacatggga
     3241 cggctggacg gcaaggttgc ggtgatcacc ggcgcgacga gcggcatcgg gctgcgaacc
     3301 gccgagattt tcgtcgcgga aggcgccagg atagtcatcg ccggtcgtcg cgtgccggaa
     3361 ggcgaggcac tggccagcaa gctcggcgcc aactgcgtct tccgccggac cgatgtcacg
     3421 gttgaaggcg agatgcgcgc gctgatcggg caggcggtcg agaaattcgg ccgccttgat
     3481 tgtctgttca acaacgccgg cggcccggcg cagaccggtg gcattgaggg cctcgaggtt
     3541 gtccgcttcg acgccgcgat ggcgaccct
//
LOCUS       DASWGM010000184         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_150356,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000184 DASWGM010000000
VERSION     DASWGM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150356"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /gene="frc"
                     /locus_tag="VGA15_05625"
     CDS             <1..1192
                     /gene="frc"
                     /locus_tag="VGA15_05625"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX9587208.1"
                     /translation="GADVIKVERPGVGDITRGQLQDVPNVDSLYFTMLNHNKRSITLD
                     TKNPKGKEVLTALMKSCDVLVENFGPGVLDRMGFPWEKIQSINPKMIVASIKGFGPGP
                     YEDCKVYENVAQCTGGSASTTGFRDGLPLVTGAQIGDSGTGLNLALGIVTALYQRTVT
                     GKGQKVTASMQDGVLNLARVKLRDQQRLAHGPLKEYSQFGEGIPFGDAVPRAGNDSGG
                     GQPGRILKCKGWETDPNAYIYFITQAAVWEKICDVIGEPGWKTHPDYAKPPARLTRLN
                     EIFARIEQWTMTKTKFEAMDILNKDDIPCGPILSMKELIEDQSLRATGTVVEVDHPER
                     GKYFSVGNPIKMSDSPTEVTRSPLLGEHTDEILRQVLGFTDHQVAEIHDSGALDPPRK
                     VAAE"
     gene            1403..1540
                     /locus_tag="VGA15_05630"
     CDS             1403..1540
                     /locus_tag="VGA15_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587209.1"
                     /translation="MSKTASFPVASSASLFNRMMVSLDRLLMAHARIAIRNGDLPYFG
                     L"
     gene            1815..>2140
                     /locus_tag="VGA15_05635"
     CDS             1815..>2140
                     /locus_tag="VGA15_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587210.1"
                     /translation="MTDTVHTTAPATARVSDAYRWTQLAIGVGAMVMIANYQYGWTFF
                     VPDIQKKFGWDRASIQWAFTLFVLFETWLVPIEGWFVDKYGPRLVVLIAGILCAIGWA
                     VNAEATS"
BASE COUNT          454 a          666 c          605 g          415 t
ORIGIN      
        1 cggcgccgac gtgatcaagg tcgagcgccc gggcgtcggc gatattacgc gcggccaatt
       61 gcaggacgtg cccaatgtcg acagtctgta tttcaccatg ctcaaccaca acaagcgctc
      121 gatcacgctc gacaccaaga acccgaaggg caaggaagtc ctgaccgcac tgatgaagag
      181 ctgcgacgtg ctggtggaaa atttcggccc cggcgtgctc gatcgcatgg gatttccctg
      241 ggagaaaatc caaagcatca atccgaaaat gatcgtggcg tcgatcaagg gtttcggccc
      301 cggcccttac gaggactgca aggtgtatga gaacgtcgcg caatgcaccg gcggctcggc
      361 ctcgaccacc ggctttcgcg acggactgcc gctggtcacc ggcgcgcaga tcggcgacag
      421 cggcaccggg ctgaatctgg cgctcggtat cgtgaccgcg ctttatcagc gcacggtgac
      481 cggcaagggt cagaaggtca ccgcttccat gcaggatggc gtcctcaatc tcgcgcgcgt
      541 caaactgcgc gaccagcagc gcctcgccca cggtccgctg aaggaataca gccagttcgg
      601 cgaaggaatt ccgttcggcg acgcggtgcc gcgcgccggc aatgattccg gcggcggcca
      661 accgggccgc atcctgaaat gcaagggctg ggagaccgat cccaatgcct atatctactt
      721 catcacccag gcggcggtct gggagaaaat ttgcgatgtc atcggcgagc ccggctggaa
      781 gacccatccc gattacgcca agccgccggc gcgcctgacg cggctgaacg aaatcttcgc
      841 ccgcatcgag caatggacca tgaccaagac caaattcgag gcgatggaca tcctcaacaa
      901 ggacgacatt ccctgcggcc cgatcctgtc gatgaaggag ttgatcgagg atcaatcgtt
      961 gcgcgccacc ggcaccgtgg tcgaggtcga tcaccccgag cgcggcaaat acttctcggt
     1021 cggcaatccc atcaagatgt cggacagccc gaccgaggtg acgcgttccc cgctgctcgg
     1081 cgagcacacc gacgaaatcc tgcgccaggt tctcggcttc accgatcacc aggtggcgga
     1141 gatccacgat tccggcgcac tcgacccgcc gcgcaaggtc gccgccgaat aggcaacaat
     1201 tccaacacct tcgaggggca gccggtttcc ggcggccctt tttgcttgcc tcggacggtt
     1261 acacccgtat aagtagtatg ctgcgttgcg aaacctaata tgctgctatg caaacaaaag
     1321 agttgttgct ggcatctggt atacctagat tgggatctaa gctacagcgt ggcaactagt
     1381 taccttcaga agagtaccaa aaatgtccaa gactgcatcc tttcccgtcg catcgtccgc
     1441 cagcttgttc aatcgaatga tggtctccct cgatcgcctg ctgatggcgc acgcccgcat
     1501 cgcgatacgc aacggcgacc ttccctattt cggcctctga tttccaaggc tgctgcggtg
     1561 gagcgacctc taccgcagcc gcattgacct gcaagcgttc ctccccgact tatggccccg
     1621 gttttccggg gccatttttt tcgttttcga gcccgtcctt ggatgggtgc gacgacgact
     1681 gcgacaaaat tgcaaaagtc atccgatcgg gggattaatc gcttgcgctc tggcatatca
     1741 cataccaaga tgtcccgact ggcaacgctc gcccactatg ataccactaa tagaagcgtg
     1801 cctcgggagg agctatgacg gacacagttc acacaaccgc tccagcaacc gcacgcgtca
     1861 gcgacgccta tcgctggacg caattggcta tcggcgtcgg cgcgatggtg atgatcgcca
     1921 actatcaata cggctggacg ttcttcgtcc ccgatatcca gaaaaaattc ggatgggatc
     1981 gcgcatcgat ccagtgggca ttcacgctgt tcgtgctgtt cgaaacctgg ctggtcccga
     2041 tcgaaggctg gtttgtcgac aaatacggcc cgcgcctggt ggttctgatc gccggcatcc
     2101 tttgcgccat cggatgggct gttaacgccg aggcaacctc
//
LOCUS       DASWGM010000185         5332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2093624,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000185 DASWGM010000000
VERSION     DASWGM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093624"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VGA15_05640"
     CDS             complement(<1..688)
                     /locus_tag="VGA15_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX9587211.1"
                     /translation="MADKDWQAGGKNLIPAQAVVCLIAAVASLGSAHGASGDFSPSSY
                     VGSADPNTIWEMLIAGIAVSSFLAAIALWVLSALRKLKRSQLRRNAFVRSALNNLSHG
                     VVMTDPQRRIVFCNDRYLQIYGLARSDLSENMTGPELLKLRLKRGVLDVSVADFYTHA
                     GTPEGLITELPGGRSVLVKYFALPNGGSVATHEDCSEQRKLSRQLASTKQFLESMLDN
                     VPVCVAAKSIE"
     gene            987..2090
                     /locus_tag="VGA15_05645"
     CDS             987..2090
                     /locus_tag="VGA15_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9587212.1"
                     /translation="MDKVECVVIGAGVIGLAVARRLAQAGREVIILEAAEGIGTVTSS
                     RNSEVIHAGIYYPAGSLMARMCVSGKRALYRYCGDHGIPHRNCGKLIVATTPKETTKL
                     QSIRAHAEANGVLDLQTLSGDAARALEPALNCDAALLSPSTGIIDSHAFMLALQGDAE
                     AAGAACAFHTPLLHAKALADRIELDAGGNAPISLECKLLVNAAGLAASAVARSINGMP
                     IDLIPAAYLAKGNYFSCSARSPFSRLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWVD
                     SIDYAVDPARAERFYPAIRRYWPTLPDGALMPSYSGIRPKIVPPAVATQDFLIQGPRD
                     HGVTGLINLFGIESPGLTSSLAIADHVGELAGM"
     gene            complement(2261..2446)
                     /locus_tag="VGA15_05650"
     CDS             complement(2261..2446)
                     /locus_tag="VGA15_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX9587213.1"
                     /translation="MAIEAHLVELERKHKVLENELHEALVHLSTDDLQILELKRRKLM
                     VKDEIERLRHTANETLH"
     gene            2833..3039
                     /locus_tag="VGA15_05655"
     CDS             2833..3039
                     /locus_tag="VGA15_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX9587214.1"
                     /translation="MTNEDERELESELARLQQEHRDLDAAIDALHQSPAPDLLRLQRL
                     KKRKLQLRDRIAFIEDQITPDIIA"
     gene            complement(3160..3540)
                     /locus_tag="VGA15_05660"
     CDS             complement(3160..3540)
                     /locus_tag="VGA15_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9587215.1"
                     /translation="MGLVESKRPVVLIVEDEFLLRMDAVDMIEAAGFEVVEAANADEA
                     IGILEARRDITVVFTDIQMPGSMDGLKLARAVRGRWPPIKIIATSGHVHVSETDLPEG
                     GRFLRKPYNPMQVMGVLRELISGS"
     gene            3889..4101
                     /locus_tag="VGA15_05665"
     CDS             3889..4101
                     /locus_tag="VGA15_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587216.1"
                     /translation="MSESSSLSDVSHYEQACDQAIAMCDGNMRSTIKALIMANEYLES
                     ELRELQAAVESGCAAAGLPHASYDAA"
     gene            4129..4380
                     /locus_tag="VGA15_05670"
     CDS             4129..4380
                     /locus_tag="VGA15_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587217.1"
                     /translation="MADVTYYVALPFLQDDTGAPVAGAAEECQSSSGALRRAEVLSKA
                     AGSVGAVAFSRTGDPMIGEFSDAKLLRKFGDVPDDLSGL"
     gene            complement(4416..>5332)
                     /locus_tag="VGA15_05675"
     CDS             complement(4416..>5332)
                     /locus_tag="VGA15_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587218.1"
                     /translation="LHYLHVPGVGPDPMPLLLLHGWPGSVFEFLELIPRLTDPARFGG
                     DARDAFTVVAPSLPGYGLSFQPSQRRFGVPEIADCVAALMHDVLGYHSFGAQGGDWGA
                     AVSSRLGYAHADRMIGIHINLMMAAGRDPSAFPDPTEEERRYLGELAHWTREESGYQW
                     IQGTRPQTLAFGLTDSPTGLAAWIVEKFRAWSDCDGDVERAIGRDRMLADISLYWFTG
                     AIGSSFWPYYARLHGSPILPAGDTIAVPTGYAQFPREILKPPRTAAARVFTDIRRWSI
                     MPKGGHFAALEQPEALAGEVRAFFRDLR"
BASE COUNT         1095 a         1621 c         1622 g          994 t
ORIGIN      
        1 cctcgatgct tttggcggcc acgcataccg gaacgttgtc gagcatcgat tccaggaact
       61 gcttggtcga ggccaattgt cgcgacagct tgcgctgctc gctgcaatcc tcgtgcgtcg
      121 ccaccgaacc gccgtttggc aacgcaaagt atttgaccag aaccgaccgt ccgccgggca
      181 gctcggtgat gaggccttcc ggggtgccgg cgtgcgtata gaaatcggcg acgctgacgt
      241 cgaggacgcc gcgcttgagt cgcaacttca gcaattccgg cccggtcatg ttttcggaaa
      301 ggtccgaacg cgccaggccg tagatctgga gataccggtc gttgcagaac acgatccggc
      361 gctgcggatc cgtcatcacc acgccatggc tgagattgtt cagggcggaa cggacaaagg
      421 cattccggcg caactgcgag cgcttgagct tgcgcaacgc cgagagcacc cacagcgcga
      481 tggccgcaag aaaagacgag acggcgatgc cagcaatcag catctcccaa atcgtattag
      541 ggtcggctga accgacgtag ctggatggcg aaaagtcgcc ggatgcgcca tgggcggatc
      601 cgagcgacgc aaccgctgcg atcaggcaca cgacggcctg cgcaggaatc aaattcttgc
      661 cgccagcctg ccagtccttg tcagccatca gtcacccgcg catttctgcg ccgcagtgtc
      721 cggcttgacg ggtttggatc gggtaaacac ctacccccgc aaaccaagaa tgtaactcaa
      781 attacggcaa ttgccctgac atgattaatg cttcactaat gggcgccagg cttgagaaat
      841 gtccggaaat gaatccatcc gactattcga aaagaccatc ccagacagag ggatagagtg
      901 ggactaggat tgatcccgga cacgcgccaa ctagtcgagg cgcgtcggat tggatagaac
      961 acaaaagatc ggatctgaac ccgcgaatgg ataaagttga gtgcgtcgtt atcggtgcgg
     1021 gggtgatcgg tcttgccgtc gcgcgccggc tggcccaggc cggccgcgag gtgatcattc
     1081 tggaggcggc cgaagggatc ggtaccgtga cctcgtcgcg caacagcgag gtgatccacg
     1141 ccggcatcta ttatccggcc ggcagcctga tggcgcggat gtgcgtcagc ggcaagcgcg
     1201 ccctgtatcg atattgcggg gatcacggca ttccgcaccg taattgcggc aagctgatcg
     1261 tcgcgaccac ccctaaggaa accacaaaac tgcagtcgat cagggctcac gccgaagcca
     1321 acggcgtgct cgatctgcaa acgctgtccg gcgatgccgc acgcgcgctg gagccggcgc
     1381 tgaactgcga tgccgctctg ctctcgcctt ccaccggcat catcgacagc cacgccttca
     1441 tgctggcgct gcaaggcgat gccgaggctg ccggtgcggc ctgtgccttt cacaccccgt
     1501 tgctgcacgc caaggcgctc gcggaccgga tcgaactcga tgccggcggc aacgcgccga
     1561 tcagccttga atgcaagctt ctcgtcaatg ccgcggggct ggctgcgtcc gcggtcgcgc
     1621 gcagcatcaa cggcatgccg atcgacctga taccggcggc ctatctcgca aagggaaatt
     1681 atttcagttg cagcgcgcgg tcgccgtttt cacgcctgat ctatccagtg cccgaacccg
     1741 gcgggctcgg cgtacatctc actttggata tggcgggaca ggcccgattc ggtcccgacg
     1801 tggaatgggt cgatagcatc gattacgccg tcgatccggc gcgggcggaa agattttatc
     1861 cggcgatccg gcgatactgg ccgacgcttc cggacggcgc gctgatgccg agctattcgg
     1921 ggattcgacc gaagatcgtg ccgccggcgg tagcgacaca ggatttcctg attcaggggc
     1981 cacgagatca cggcgtcacc ggactgatca atttgttcgg gatcgagtct ccgggcctga
     2041 catcatcgct ggcgatcgcg gaccatgtcg gcgaactggc ggggatgtga gcgcatcctg
     2101 gagtggcaga ccggctcaat atcaaaccag ctcaatatca aacgctgtca tgctccgcca
     2161 cccggtggcg ctatgcgcgg acccgttggc ggaccatgac agcagtattt ggacagcggt
     2221 atttgggggg cctgtaatag actggctggt gtgcagcctg tcagtgcaat gtttcgttgg
     2281 cggtatgtct cagccgttcg atctcgtcct tgaccatcag ttttcggcgc ttcaactcaa
     2341 gaatttgcag gtcgtctgtt gagaggtgca cgagagcttc gtgcaattcg ttttcgagaa
     2401 ccttgtgttt ccgctccagt tcaacgagat gcgcctcaat tgccatacga aacctcctct
     2461 gtgggattaa gattccatcc ggaccagcga ttgtacacga gccgacgaag ctgtcgatgg
     2521 gtatcaaaat tggcgttctc atatttgagc gtcatatgta atgaagcgtg agcatggaac
     2581 cggtctggtg tgcagcgcga aagccgcggc gcccgcttga gcgaacgtcc gattcgaagg
     2641 caagccggaa acccctgatt ccagtgcgat tttgcttcaa atttcgctga accgaccggc
     2701 ggccgaaaat aaagaagaac cgctgagcca ccaccacatc agcttgcaga ccgccctcgc
     2761 agggacgata ttccgaccac aggcttccac aaccgctcac aacctcatcg cgtttttcag
     2821 ctaacgtaca ccatgaccaa cgaagatgag cgtgaactcg aatccgagct cgcacggctg
     2881 cagcaggagc accgcgatct cgatgcggcg atcgatgccc tgcatcagtc gccggcgccg
     2941 gatctgttgc ggctgcaacg gctgaagaaa cgcaagctgc agctgcgcga ccgcatcgcc
     3001 ttcatcgaag accagatcac gcctgacatc attgcgtagg ttttgtcgtt ccgagatgcc
     3061 cgtttggggc agacctcgga tgtgcaattg cacaccgggg aatccttgga ttccgggttc
     3121 gctcgtttca ttcgcgcccc ggaatgacag ttttgaaatt cagctgccgc tgatcaattc
     3181 ccgcagcacc cccatcacct gcatcggatt gtagggcttc cgcagaaacc gtccgccttc
     3241 aggcagatcg gtttcgctga catggacgtg gccggaggtt gcgataatct tgatcggcgg
     3301 ccagcgaccc ctgaccgctc gcgcgagctt caacccgtcc atggaacccg gcatctggat
     3361 atcggtaaaa accacggtga tgtcccggcg agcttccaga attccgatcg cttcgtcggc
     3421 attcgccgct tcgaccacct cgaacccggc ggcttcgatc atatcgaccg cgtccatgcg
     3481 caacagaaac tcgtcctcga cgatcagcac cacgggtctt ttggactcaa cgagacccat
     3541 gcctttcact cccctacgaa cacccgttaa gtcccggatt ttgttttggt tccaccgcta
     3601 acttgttgcg gggactgcgc cgagggctgt tcgtggcgac cgacctcagc gcgtacctcg
     3661 ccgccccgaa gggcgattca cgtttcggat gccgctttaa gtgaaatcga tatcggcaga
     3721 atccggctgc gcggtggcct ttgcaaaaga ggccgccggc cgggaaagcg cctcgcaaac
     3781 tgggccaatc tcggatcagg acccggcttc aaattcgcac agggccactt gactccatga
     3841 gaacaaaata ggaacatcgg gacgtctcca gccatcggga atttcgtcat gtccgaatcc
     3901 tcttcactgt ccgacgtcag ccattacgag caggcctgcg atcaggcgat cgccatgtgc
     3961 gacggaaaca tgcgcagcac catcaaggcg ctgatcatgg cgaacgaata tctggaaagt
     4021 gaattgcgtg aattgcaggc ggcggttgag agcggctgcg ctgcggcagg attgccgcac
     4081 gcaagttacg acgccgcctg atcgcggaat caaacctgga gccagaccat ggccgatgtc
     4141 acctactacg tcgcgctgcc attcctgcag gacgataccg gcgcgccggt cgcgggggcg
     4201 gcggaagagt gccagagttc gtcaggcgcg ttgcggcgcg cggaagtgtt gtcgaaggcc
     4261 gccggcagcg ttggtgcggt cgccttcagc cgcaccggcg atccgatgat cggcgaattc
     4321 agcgacgcta aattgctgag aaaattcggc gacgtgccgg atgatctgag cgggctctga
     4381 tgaattcgct tccggatcac acttcgtgcg cggcatcacc gcaagtcccg aaagaacgcc
     4441 cgcacctcgc cggcgagcgc ctccggctgt tccagcgcgg caaagtgtcc acccttgggc
     4501 atgatgctcc agcggcgaat gtcggtgaac acgcgcgccg cggcggtccg cggcggtttt
     4561 agaatttcgc gcgggaattg cgcgtagccg gtaggcaccg caatcgtatc gcccgcgggc
     4621 aggatcggcg aaccgtgaag acgggcataa tacggccaga acgacgagcc gatcgccccg
     4681 gtaaaccaat agagcgagat gtcggcgagc atgcggtcgc ggccgatcgc gcgctcgacg
     4741 tcgccatcgc aatcagacca ggcgcggaat ttctccacga tccaagccgc caggccagtg
     4801 ggcgaatcgg tgagaccaaa ggcaagcgtc tgggggcgtg tgccctggat ccattgatag
     4861 ccgctctctt cgcgggtcca gtgcgcaagt tcgccgaggt aacgccgctc ctcctcggtc
     4921 ggatcgggaa aggcggaggg atcgcggccc gccgccatca tcagattgat gtggatgccg
     4981 atcatccggt ccgcatgcgc atagccgagc cggcttgaga ccgcagcacc ccagtcgccg
     5041 ccttgcgcgc cgaagctgtg atagccgagc acgtcatgca tgagggcggc gacgcaatcc
     5101 gctatctcgg gaacgccgaa ccgcctctgg ctgggctgga aagaaaggcc gtagcccggc
     5161 agggacggcg ccaccacggt gaaggcgtcg cgcgcgtcgc cgccgaaccg tgccggatcg
     5221 gtgaggcggg ggatgagctc gagaaactcg aacaccgagc cgggccagcc gtgcagcaac
     5281 agcagtggca tcgggtcggg gccgacgccg ggcacatgca aatagtgcag gt
//
LOCUS       DASWGM010000186        10203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_566434,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000186 DASWGM010000000
VERSION     DASWGM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566434"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VGA15_05680"
     CDS             complement(<1..452)
                     /locus_tag="VGA15_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5765 domain-containing protein"
                     /protein_id="HEX9587219.1"
                     /translation="MCWNGQASAALATVGLGTTAYAAYRKEPAAIWMPLGYFSLMELL
                     QAFTYSVIDQCSLPSNQIATLLGYLHIAFQPFFINAVSMHFIPDQARARIAPVVYSLC
                     FVSAVFMLLQLYPFAWAGHCDPSTPLCGTRLCSVHGNWHIAWQVPTNG"
     gene            complement(546..857)
                     /locus_tag="VGA15_05685"
     CDS             complement(546..857)
                     /locus_tag="VGA15_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587220.1"
                     /translation="MREARARRRRRVSKNTIPFGAAYESAIDPYYRELYCDEYVKLAP
                     STTGDRRAETGHLIAVVDLMPNSWASPQGEAKKCNVHDRTVMMNALAIKEAGACRRAA
                     T"
     gene            1083..1700
                     /locus_tag="VGA15_05690"
                     /pseudo
     CDS             1083..1700
                     /locus_tag="VGA15_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(1821..4148)
                     /locus_tag="VGA15_05695"
     CDS             complement(1821..4148)
                     /locus_tag="VGA15_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9587221.1"
                     /translation="MNILPGNMRFGAGQPVKRLEDQRLLTGKGLFIDDRPEDGALWLH
                     VLRSPHAHAKIVSIDINAAREMPGVEAVYTGADLVADDIGTLPTLLVFKRPDGSPMTA
                     PPRRLLAHEIVRFAGEPVAAVVATSRVVAQTAAEAIVVDYDVLPSVVDPVAAVKPGAP
                     AVWAEVPDNIAAAMSYGDAAAVEASFASAAHVVSLDLISQKLIPSAMEPRSTIAEIEK
                     KTGRLILHVQSQTPGSTRDLLAEAVLKRPKESVRVLVGDIGGGFGQKTSLYPEDGMVA
                     YAAVKLSRKVRWRGDRTDEFVGGTHGRDLTSTGEFALDAKGRVLAYRVRSLGGTGAYL
                     TGTGTIIPLVLGPFVQSGVYDLPLVHYEVKAVMTNTAPVGAYRGAGRPEGVFIVERLM
                     DAAARQIGMDPREIRKINYIKPAQLPYTNAVGQVYDSGACAHMLERASKLADWDGFAA
                     RKKAAKKKGLLYGRGLTSYIEWTGGRAHTEKVSLHATAEGRIILHSGTQAMGQGLQTT
                     YSQMIAGSLGISMDKIEIVQGDTDLAIGFGSVGSRSLFVGGTAVAVSSNDLIAKAREK
                     ASHLLEAAVEDIEYRDGMLTVVGTDKRIGMFDIAKDEKGARLSVDSEGEVDGPSWPNG
                     THICEVEIDPETGVSKVVRYTTVDDVGVAVNPMLVTGQVHGGVAQGIGQALYEGVSYD
                     PEGQLLTASYQDYCVPRADDIPPIEVTLDDSAPCRTNPLGAKGCGESGAIGGPPCITN
                     GVMDALSELGIKNLNTPLSPLKVWQAIQEAKAARV"
     gene            complement(4415..5290)
                     /locus_tag="VGA15_05700"
     CDS             complement(4415..5290)
                     /locus_tag="VGA15_05700"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9587222.1"
                     /translation="MADFHGVFPYLVSPVDTDGKVRTDVLGRLCDDLIKSGVHGLTPL
                     GSTGEFAYLNNAQRMAVVQTTIEAAKGRVPVVAGVASTATADAVAQAKFYQKLGADGI
                     LAILEAYFPLGDAQVECYFRAIADAVDIPVVIYTNPQFQRSDLTLDVIARLAAHPRIG
                     YIKDASTNTGRLLSIMNRCGDAIRVFSASAHIPAAVMLIGGVGWMAGPACIIPRQSVE
                     LYNLCKAARWDEALTLQRRLWRINEAFARYNLAACIKAELAIQGYDVGDPLPPQAALS
                     ADERKAVEAVLRDFG"
     gene            complement(5283..6263)
                     /locus_tag="VGA15_05705"
     CDS             complement(5283..6263)
                     /locus_tag="VGA15_05705"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX9587223.1"
                     /translation="MSFLTKLPAELYNPDAFKQFSGGTEFDIGNARAMAWMSQLAYET
                     DEPDKIKGILKLFGLNLVEGGVVVRESKTVLPVASTHCFVASHPKAVIVAFAGTDPVS
                     LANWISDFDAHLDVTTGTAEGYEVAAAVVWPELKQLLEQCVPPGGKVIVTGHSLGGAL
                     AALIANRIGTELQADVDAVYTFGMPRPGNKAFADLYNQQLGMRTYRLVHGEDIVPTVA
                     PTSFGFRHLGRVLHCERLGRFDKANLASDTSSDFPPFVSGVAKDLRDHLQGPLSAVLS
                     IAARIKLAAALLTGMGPSNMRTDPGGILIEMLPPRLRDHMPDRYIGACNG"
     gene            complement(6492..7109)
                     /locus_tag="VGA15_05710"
     CDS             complement(6492..7109)
                     /locus_tag="VGA15_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587224.1"
                     /translation="MTTNMALKRARKAQRRKQMVAQKRRAEILEASLPARVLRAAHAP
                     IQHCFLTESVFDVGMGTLVLARGATLHHFAFSMFLIDVFCLGIKDVMFESVESEVFEM
                     YMDATDAGSPMVSVDPSYARKLLRDLAAWSQSIGFAPHRDFAAVERVFGDVSVDTSDA
                     VFRFGRDGKPVYIPGPNDTAHLIQRRIEQLQKYLGDDGFGFEAAA"
     gene            7276..7647
                     /locus_tag="VGA15_05715"
     CDS             7276..7647
                     /locus_tag="VGA15_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587225.1"
                     /translation="MNLSADALYKMSDVELLAAYVEARRQFVEKKFTRDTHRARLAWI
                     KAKMFIGSLGGVTERNMAIDVSEEIARKGQELREMTRDLDLLKVDVDIIAIVIRFRGA
                     SAPTGVQGDDAIEGDPEREGP"
     gene            8472..8978
                     /locus_tag="VGA15_05720"
     CDS             8472..8978
                     /locus_tag="VGA15_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587226.1"
                     /translation="MVEALVSNLYDHFRDEGATDDLGNLCYSAQVTGEPSPVDPCQSL
                     SEPEQIIPVRIEVAPDRMTWRALYCPRRKQIVGPHSKFVADKVQHSFELVRRNDRLHL
                     VHADGADRTLCGEPAAGEIIPFFSYDYPNKPDDPSEPAPRDLYAEWSDGVVCQQCLLH
                     DQALNRQC"
     gene            9169..9612
                     /locus_tag="VGA15_05725"
     CDS             9169..9612
                     /locus_tag="VGA15_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587227.1"
                     /translation="MPVSFQEILTAFEFVGMGGDLGAHQAILCRRTGKIYWRSEFSDL
                     DELTELNDEMPDDVEDDDEKYIAIPDKRELGLGKPLALDFAREFLPSDFDEVRYIFSK
                     RGAYKKFRALLIRRNVLDSWYDFESKATEQALREWCELNSIEVAG"
     gene            complement(9721..>10203)
                     /locus_tag="VGA15_05730"
     CDS             complement(9721..>10203)
                     /locus_tag="VGA15_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9587228.1"
                     /translation="ALSGGFYPVSAVLSSNAVLGTLKPGQHGSTFGGNPLACAVARAA
                     LRVLVEEGMIENAAVQGARFLDGLRSIRTNTIREVRGRGLMLAVELHPEAGDARLYCE
                     ALQSRGILAKDTHGHTIRIAPPLVITSDQVDWALERIAAILRHDFSSWRCSANEQRNR
                     "
BASE COUNT         1972 a         3146 c         3158 g         1927 t
ORIGIN      
        1 ataccgttgg tgggaacctg ccaggcgatg tgccagttgc cgtgcaccga gcaaaggcga
       61 gtgccgcaca acggcgtgga gggatcgcaa tggccggccc aggcgaatgg atagagttgc
      121 agcagcatga aaacggcgga cacgaagcac aacgaataga cgaccggtgc aatccgcgcc
      181 cgcgcctggt ccggaatgaa atgcatcgac actgcattaa tgaagaacgg ctggaaggcg
      241 atgtgcagat agccgagcag ggtcgcgatc tgattggatg gcaggctaca ctgatcgatg
      301 accgagtagg tgaaggcctg cagcagctcc atcagcgaga aatagcccag gggcatccat
      361 atcgccgccg gttccttgcg gtaggctgca taggcggtcg tgccgagacc taccgttgcg
      421 agcgctgccg atgcttgacc gttccagcac attgatcact cctgagggaa ggccgacgcc
      481 gcgcgtcggc gtcgtgaacg cgagacggga cccgctcgag ttctatcgaa cgggcatcac
      541 gccgatcacg tcgctgcgcg tctgcacgcc ccggcttcct tgatcgcaag cgcgttcatc
      601 atcaccgtcc tgtcgtgcac gttgcacttt ttcgcctcac cttgcggtga ggcccaggaa
      661 ttcggcatga gatcgacgac agcgatcagg tgtccggttt cagcgcgccg atcgccggtc
      721 gtcgaaggag cgagttttac atattcatcg caatagagct cccggtaata cggatcgatg
      781 gcagactcat aggccgctcc gaacggtatc gtatttttcg aaacgcggcg ccgccgcctt
      841 gctcgggcct cgcgcatctc gtcaagaacg gtcccactga aatgcgcatt ctttcgacgt
      901 cgaaaatgca ttacgtctca caaattacat caccgctacg gcttcaacat tcaggcagca
      961 tagatacgag tgtcccgcaa gacttgggca gaagcgacat ctcgacggac gacggcgaac
     1021 tgccaaaccc tttggcacgc gcctgtacgc accgccgcct taccctagcg tgatcgatcg
     1081 ctatgaagag cccgggcatc accacccgtc cgatcctgac catcggcggc gatcacgagg
     1141 tcaaccaggt gttcttcgaa gacgtgcgcc tgcccgccgc caaccgcgtc ggcggggaag
     1201 gcaagggctg gacctatggc aagtatctgc tcgagttcga acgcggctcc ggcattgcct
     1261 cggccaaatt gcgcgaagcg cagaaaaccg tatcggacct ggcgacatcg gacgtgaccg
     1321 gccgcgccat cgacgatccc gatattgcct tgcgaatgtc cgagatcgaa gtcgatatcg
     1381 acacactgga gatgacggag ctgcgggtgc tgtcggcgcc gcagaccgga caaaatccgg
     1441 gcgcggtatc ttcgctgctc aaattgcggg tcagcgaaat ccgccaggcg gtgacgcggc
     1501 tccgcgtcga ggtgatcggc aacgacggcc tttatgtcga gccgatgcgg ccgttctaca
     1561 aactcaacga gaagcctgcg atccccaaag cgatgttgcc ggtggtgccc gaatatctca
     1621 acggccgcgc ctacaccatc ttcggcggct catcggagat ccagcgcgac atcatcgcga
     1681 agatggtgct ggggctgtag gactctcaac tcgtcattcc ggggcaatgc gatagcatcg
     1741 aacccggaat ctcgagattg cccgatgtgc cattgcacat ctgaggtctg gtccttcgga
     1801 ccatcccgga atgacagcaa ctacaccctt gccgctttcg cttcctggat cgcctgccag
     1861 actttcagcg gcgacagcgg cgtgttgaga ttcttgatgc cgagctcgct gagcgcgtcc
     1921 attacgccat tggtgatgca gggcggcccg ccgatcgcgc cggattcgcc gcaacccttg
     1981 gcgcctagcg gattggtgcg gcagggtgct gaatcatcca gcgtcacctc gatcggcgga
     2041 atgtcgtcgg cacgcggcac gcagtaatcc tgatagctcg cggtcagcaa ctgcccttcg
     2101 gggtcgtagg acacgccctc gtacagcgcc tgtccgatcc cctgcgcgac gccgccgtga
     2161 acttgccccg tcaccagcat cggattgacg gcgacgccga cgtcgtcgac cgtggtgtag
     2221 cgaacgacct tgctgacgcc ggtctccgga tcgatttcga cttcgcagat atgcgtgccg
     2281 ttcggccagc tcggcccgtc gacctcgcct tcgctgtcga cgctcagccg cgcgcctttt
     2341 tcgtccttgg cgatatcgaa cataccgatc cgcttgtcgg tgccgaccac cgtgagcatg
     2401 ccgtcgcgat attcgatgtc ctcgaccgcg gcctccagca ggtgcgacgc cttctcgcgc
     2461 gctttcgcga tcagatcgtt gctcgatacc gctaccgcgg tgccgccgac aaacagcgag
     2521 cgcgaaccga cgctgccgaa tccgatggcg agatcggtgt cgccctggac gatttcgatc
     2581 ttgtccatgg agatgccgag cgatccggcg atcatctgcg aataggtggt ttgcaacccc
     2641 tgccccattg cctgcgtgcc cgaatgcagg atgattcggc cttcggcggt ggcgtgcagg
     2701 ctgacctttt cggtatgcgc gcgcccgccg gtccattcga tatagctggt caggccgcgg
     2761 ccgtagagca ggcctttctt cttggccgcc ttcttgcggg cggcaaagcc gtcccagtcg
     2821 gcaagttttg acgcacgctc cagcatgtgg gcgcaggcgc cgctgtcgta tacttggccg
     2881 acggcgttgg tatagggcag ttgcgcgggc ttgatgtaat ttatcttgcg aatctcgcgc
     2941 gggtccatgc cgatctggcg cgctgcggca tccatcagcc gctcgacgat gaacacgccc
     3001 tcgggacggc ccgcgccgcg ataggcgccg accggtgcgg tgttggtcat cacggccttg
     3061 acctcgtagt gaaccagcgg caggtcgtac acgccggact gcacgaacgg gccgagcacc
     3121 aaaggaatga tggtgccggt gccggtgaga taggcgccgg tgccgccgag cgagcgcacc
     3181 cgataggcca gcacgcggcc tttggcgtcg agcgcgaatt cgcctgttga agtgaggtcg
     3241 cggccatggg tgccgccgac gaactcgtcg gtgcggtcgc cgcgccagcg caccttgcgg
     3301 ctaagcttga ccgcggcgta ggccaccatg ccgtcttccg gatagaggct ggtcttctgg
     3361 ccaaaaccgc cgccgatatc gccgaccagc acgcgaacgc tttccttcgg ccgcttcagc
     3421 accgcctcgg cgagaaggtc gcgggtcgag cccggcgttt gcgattggac gtgcaggatc
     3481 agccgtccgg tcttcttctc gatctcggcg atggtgctgc gcggctccat cgccgagggg
     3541 atcagtttct ggctgatgag gtcgagcgag accacatgcg cggcgctggc gaacgatgct
     3601 tcgaccgcgg ccgcgtcgcc atagctcatg gcggcggcga tattatcggg cacctccgcc
     3661 cacaccgcgg gcgcaccggg cttgacggcg gcaaccggat cgaccaccga cggcagcacg
     3721 tcataatcaa ccacgatcgc ttccgcggcg gtctgcgcca cgacccgcga tgtcgccacc
     3781 accgcagcga ccggctcgcc ggcaaaacgc acgatttcgt gcgccagcag ccgccgcggc
     3841 ggcgccgtca tcggcgagcc gtcgggccgc ttgaaaacca gcagcgtcgg aagcgtgccg
     3901 atgtcgtcgg cgaccagatc ggctcctgtg taaaccgcct cgacgccagg catttccctg
     3961 gcggcgttaa tatcgatcga cacgatcttc gcgtgggcat gcggcgagcg cagcacatgc
     4021 agccacagcg cgccgtcttc cggtctatcg tcgatgaaca ggcccttccc ggtgagaagt
     4081 cgctgatctt cgaggcgctt gacgggctgg cccgcgccga aacgcatgtt gccgggaaga
     4141 atattcatca gtttgtcctt cgagtctttt gtaggattgg gcgggtttta gtcgatctgc
     4201 ttagtcgacc tgcaaggcac aggccaaccc ctgtgtgagg gtgacaatca ttagaagccg
     4261 tcatggccgg gctacgcccc ttgctccgtc attccgggat gcgccgcaag gcgcagaccc
     4321 ggaatctcga gctgatcatc tctggattcc gggttcgcgc ttcgcgcgcc ccggaatgac
     4381 gacgtgctgg tatttgccgc ggcagacaag ccaatcaccc gaagtcccgc aacaccgctt
     4441 cgaccgcctt gcgctcatcc gcgctcaatg cggcctgcgg tggaagcgga tcgccgacgt
     4501 cgtagccctg gatcgcaagc tcggccttga tgcaggccgc cagattgtag cgcgcgaagg
     4561 cttcgttgat gcgccacagc cgccgctgca aggtcagcgc ctcgtcccag cgcgcggcct
     4621 tgcagagatt gtagagctcc acgctctggc gcggaatgat gcaggccggt cccgccatcc
     4681 atccgacccc gccgatcagc atcaccgcgg ccgggatatg cgcggaggcc gagaacacgc
     4741 ggatagcgtc gccgcagcgg ttcatgatcg acaacagccg cccggtattg gtggaggcat
     4801 ccttgatgta gccgatccgc ggatgggcgg cgagccgcgc gatcacgtcg agcgtcaggt
     4861 cggatcgctg aaactgcgga ttggtgtaga tcacgacggg aatatcgacc gcatccgcga
     4921 tggcgcgaaa atagcattcg acctgcgcat cgccgagcgg gaaatacgct tcgaggatcg
     4981 ccagaatgcc gtcggcaccc agcttctgat agaacttcgc ctgcgcgacg gcatcggccg
     5041 tcgcggtcga tgcgacaccg gcgaccaccg gcacgcggcc ctttgccgct tcgatcgtgg
     5101 tctgcaccac cgccatgcgc tgcgcgttgt tcagataggc gaactcgccg gtcgagccga
     5161 gcggcgtcag cccatgcacg cccgatttaa tgagatcgtc gcagagacgg ccgagcacat
     5221 cggtgcggac ttttccgtca gtatcgaccg gcgagaccag atagggaaag acgccgtgga
     5281 aatcagccat tgcacgcgcc gatgtagcgg tccggcatgt gatcgcgcag ccgcggcggc
     5341 agcatctcga tcagaattcc tccggggtcg gtacgcatgt tcgaaggtcc catgccggtc
     5401 aggagcgccg cggccagttt gatccgcgcc gcgatcgaca gcaccgcgga aagcggccct
     5461 tgtaaatgat ccctgaggtc cttggcaacg ccgctgacga aggggggaaa atccgaactc
     5521 gtatcggagg ccagattggc tttatcgaac ctgcctagtc gttcgcagtg cagaacccgc
     5581 ccaagatgtc tgaaaccaaa cgaagtgggc gcgacggtcg gcacgatatc ttcgccgtgc
     5641 accagccgat aggtgcgcat ccccaactgt tgattgtata gatccgcaaa cgccttgttg
     5701 cccgggcgcg gcatgccgaa ggtgtagacg gcgtcgacgt cagcctgcaa ctcggtgccg
     5761 atccggttcg cgatcagtgc cgcgagcgcg ccgcccaggc tgtgcccggt gacgataacc
     5821 ttgccgcccg gcggaacgca ttgctcaagc aactgcttca gttcgggcca gacgacggcg
     5881 gcggcgacct cgtagccttc agccgtgccg gtcgtcacgt ccagatgcgc atcgaagtcc
     5941 gaaatccagt tggccagcga gaccggatcg gtgccggcga atgcgacgat cacggccttc
     6001 gggtggctgg cgacgaagca atgggtgctc gcgaccggca gtaccgtttt cgattcgcgc
     6061 acgacgacgc cgccctcgac tagattgaga ccgaacaatt tcagaatacc tttgatcttg
     6121 tccggctcgt cggtttcgta agccagttgc gacatccacg ccatggctct ggcgtttcca
     6181 atgtcgaatt ccgtcccgcc ggaaaattgt ttgaaggcgt ccggattata aagttcggcc
     6241 ggaagtttgg tgaggaagct catgttacgg ctccatcgct cgctacatgc agaacttgca
     6301 ttcgggcgat cttacacctg caacaacgaa ggccaatggg agtctcttgc gtgattgcac
     6361 gagcgggcca gcagagcggg cgcccgagac agtgagttcg agcacgggaa ttgtgaaagg
     6421 tcaccgctgg ctgattgctg gcttgcaggg catgtcccgg gcgtcgtaac gacgagggca
     6481 caagatcggc ctcaggccgc cgcctcgaaa ccgaagccgt catcgccgag atacttctgc
     6541 agttgctcga tccggcgctg aatgagatga gcagtgtcgt tcgggccggg aatatagaca
     6601 ggcttgccgt cgcgcccgaa ccgaaagacc gcgtcgcttg tgtccacgct cacgtcgccg
     6661 aacacccgct ccaccgctgc gaagtcccgg tgcggcgcga aaccgatcga ttgcgaccac
     6721 gcggcaagat cgcgcagcag cttgcgggcg tagctcggat cgacggaaac cataggcgac
     6781 ccggcatccg tagcatccat atacatctcg aaaacttcgc tttcgaccga ttcgaacatc
     6841 acatccttga tgccgagaca gaacacgtcg atcagaaaca tgctgaacgc gaagtgatgg
     6901 agtgtggcgc cgcgcgccag aaccagcgtc cccatgccga cgtcgaacac agattcggtg
     6961 aggaagcagt gctgaatggg cgcatgggca gcccggagca cgcgcgcggg caggcttgcc
     7021 tccaatatct cggcccgtcg cttctgggcc accatctgtt tccgccgttg cgctttcctg
     7081 gcgcgcttca gcgccatgtt cgtcgtcatc gcctattcct tgagtgattc gaattgctgt
     7141 atgatacagt agcagattcc acttggtaga gtggtggcgc atcgaatatt ctggggataa
     7201 ggccccgatc gaaattgcgg gccggcgcgt ccggccgccg agttcaccac gatcaacaaa
     7261 tctcagcccg gtgccatgaa cctttcagcc gacgccttgt acaagatgtc cgacgtggaa
     7321 ttgctggcgg cctatgtcga ggcccggcga caattcgtgg aaaagaagtt cacgcgagat
     7381 acacatcggg cgcggctggc gtggatcaag gcaaaaatgt tcatcggcag cttgggcggg
     7441 gtcaccgagc gcaacatggc catcgacgtc tcagaagaga ttgcccgcaa gggtcaggaa
     7501 ttgcgtgaga tgacgcgcga cctcgacctg ctcaaggtcg atgtcgacat catcgcgatc
     7561 gtcattcgat tccgcggcgc atcggcgccg accggcgtgc agggcgacga cgcgatcgaa
     7621 ggcgaccccg agcgcgaagg cccttgagct gcctcgtccg accgggagcg cgtgatgctc
     7681 gatgttcgcg tggcgggctt gcgaaatcga ttccccggaa gcaggcaaga agccgctggc
     7741 caaggagcac gccctcatcc cattggcgct tgcggtacgt gaagcacacg caaggcgttc
     7801 gcgggctgtg gcatcaggca caaattggtg ccgagcggcc tacgccgcat catttgcaat
     7861 cggcagcggc aaccggtgtt gcgtgatgga cagacacccc gcgccgctca gatcgcaacc
     7921 gatctcgagc gcttcccgcg aagtgtgcgc tggcgtctag ccgtggatga atggcatgga
     7981 tcaatagatt agatggcgtg gcgcgccatt cagaatgcaa cggcaaacac atacgtcgtt
     8041 gaagttgctg tgtaattcga acgcgggccg gattggcgcc ggatcggctt cgcgtgctgc
     8101 tgggcgggac acaagaaatg gtcgacggat gacgagcgca ttacgcgcca tgacttcact
     8161 gaatcgcgca gggcgcgcga tgatcgtcgc ctgcacgcgc accggcgtcc ctgccgatgt
     8221 gttgattcgc ccgggcggac aaggcctggt caatggcggt caccgggtca tattgcggtc
     8281 atgactttcg cgctcttggc gcttctcgtg tcgcgcgacg ttgacgctgc ttcctcgaca
     8341 aggtccggaa gcgtggattg aaaacaagcg ttggtctggg gacctggttg gtatggggac
     8401 ttcgtatggg gacctggctt gcccgtgtgc actttccaga tgaacatgtc cgctaagcga
     8461 agtattcaac cgtggtggag gcgctggttt ccaaccttta tgatcatttc cgtgacgagg
     8521 gcgcgaccga cgatttagga aacctctgtt atagcgcgca ggtcacgggc gagccgtcgc
     8581 cggtcgatcc ctgccaatca ctgtcggagc cagaacagat cattccggtg cgtattgagg
     8641 tcgcgcctga tcgcatgact tggcgtgcgc tctattgccc tcggcgaaag cagattgtcg
     8701 gtccgcactc gaaatttgtt gccgacaagg tgcagcacag cttcgaattg gttcggcgga
     8761 atgatcggct tcaccttgtc cacgccgacg gagccgaccg gacattgtgt ggggaaccgg
     8821 ccgccggcga gataatccct ttttttagct atgactaccc gaacaaaccc gatgatccgt
     8881 cagagcccgc gcctcgcgac ctttatgccg agtggagcga tggagttgtt tgtcagcaat
     8941 gccttctgca cgatcaggcg ctgaacaggc aatgctgagg tttgcgccct gcaggagacc
     9001 gttgacaggc gcaggcatat cgccgagatt ctgaaccgaa aatacggact atcgaccgcg
     9061 atgatcgtcg cctgcacggg cgcatcggca tctccgccga tgtgctgatc cgcccgagcc
     9121 gaaagagggc ggcgtgaccg gcgccattgc atttcgccag gcagaccaat gcccgtcagc
     9181 tttcaggaaa tactcacagc gttcgaattc gttggaatgg gcggagacct cggcgcgcat
     9241 caggccatcc tgtgcaggcg caccggcaag atctattggc gctccgaatt ttccgacctg
     9301 gacgagttga ctgagctgaa cgacgagatg cctgatgacg tcgaggatga tgatgagaaa
     9361 tacatcgcga tccccgacaa acgagaactc ggtctcggca agccgctggc gctggacttc
     9421 gcacgcgaat tcctgccaag cgacttcgat gaggttagat acatcttcag caagagaggc
     9481 gcctacaaga aatttcgagc gctgctgata cgaagaaacg ttcttgattc ctggtatgat
     9541 ttcgaatcga aagccacgga acaagcgttg cgcgagtggt gcgagctcaa ttcgatagaa
     9601 gtcgccggct gatgccttgc aacgcggtag caaggctggg aacattggcc cgcggggcgc
     9661 gacgcattac ccacctgcga cccgccgtca tttccaaccg acaccgcccc gcgtgagccg
     9721 tcatctattc ctttgctcat ttgccgagca ccgccatgac gagaaatcgt gcctcaaaat
     9781 ggctgcgatc ctctccagcg cccagtcgac ctgatcgctg gtgatcacaa gtggcggggc
     9841 aatgcggatg gtgtgtccat gggtgtcctt ggcaaggatg cctctgctct gcagcgcctc
     9901 acagtagaga cgggcatcgc ccgcttcggg gtgaagctcg accgccagca tcagcccgcg
     9961 tccgcgcacc tcacggatcg tgttggtgcg aatgcttctg aggccatcga ggaagcgcgc
    10021 gccctggacg gctgcattct cgatcatccc ttcctcgacg aggacgcgca gggctgccct
    10081 cgccaccgca caggccagcg ggttgccgcc gaaggtcgag ccgtgctggc cgggtttcag
    10141 ggtgccgagc accgcgttgc tcgaaagcac ggccgacacc gggtagaagc ctccggacaa
    10201 cgc
//
LOCUS       DASWGM010000187         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1817264,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000187 DASWGM010000000
VERSION     DASWGM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817264"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VGA15_05735"
     CDS             <1..1372
                     /locus_tag="VGA15_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587229.1"
                     /translation="RRGQPTRDQVVTSFPSKSEIALNLSTDGRVVTFMGYLAPINALD
                     VSNSNTPAVVDPTNPVPGSYSRVVAEVDAHGRFRFTKTNAYSGNNGRAAILNDKNGAR
                     IVYTVGNAGNGANPQPDGIIVGAGAQILTAERTPLAAQNPGLPTPVGSFNITELGLKA
                     DKIGKDTNFRGLTIFNNVVYTTKGSGSNGINTVYFIDTTGFDANGKPLACPNGVGLPG
                     ATATLPTAPIFYDATKLQTLGVVPYNMCILKGFPTTLAKTATSFPFGVWFADAKTLYV
                     ADEGNGTNTFDPASGKYTAAATQTGAGLQKWVFDTGLGAWNLAYVLQAGLNLGVPYTV
                     GNYPTGQNPKTGLPWAPAADGLRNIVGRVNRDGTATIWAITSTVSGNGDQGADPNQLV
                     MITDHLAATSASAGESFATIRTARFGEVLRGVSFTPGTDRDRLADADDHRADADDHRA
                     DRDDRH"
     gene            complement(1472..2278)
                     /locus_tag="VGA15_05740"
     CDS             complement(1472..2278)
                     /locus_tag="VGA15_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9587230.1"
                     /translation="MTLSLTILGCGSSAGVPRPALGWGACDPGNPKNRRRRCSLLAER
                     TSDNGVTRIVIDTAPDLREQLIDANVDHIDAVFLTHEHADQTHGIDDLRSVVLHQRRR
                     IPVYLNQSTANHVLLRFSYCFVAPPGSDYPPILDNHPIEAGESRTIEGKGGAMTLSAF
                     ILQHGNIPALGYRIGDAAYTPDASDIPPESWPYLKNLDLWIIDGLRYAGHPSHFSVND
                     ALSWIDRFKPRRAVITNMHSDLDYEVLRQSLPPTVIPAYDGMRLTLERAD"
     gene            complement(2275..3066)
                     /locus_tag="VGA15_05745"
     CDS             complement(2275..3066)
                     /locus_tag="VGA15_05745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9587231.1"
                     /translation="MLVDSHCHLDFPDFADDLDAIVARAAAAGIGRIVTISTRVKRLR
                     ELLDIAERFPDVYCSVGTHPHHADEEDGIPADELVELTKHPKVVALGEAGLDNFYQHG
                     SREAQERGFRAHIAAARKTGLPLVIHTRDADDDCGRVLEDEMTKGSFRAVLHCYTGGR
                     ELAMKAISLGLSISFTGILTFKKSQGLRDLAAELPADRIMVETDSPYLAPGKFRGKRN
                     EPSYVVEVAKVLAETRGVSLEEISRQTTENFFRLFSKVPSAKAMA"
BASE COUNT          630 a         1005 c         1015 g          594 t
ORIGIN      
        1 gcgccggggc caaccgacaa gggatcaggt ggtcaccagc tttccgtcga aatcggagat
       61 cgcgctcaac ctgtcgaccg atggccgggt cgtgacgttc atgggttatc ttgcgcccat
      121 caatgcgctc gatgtctcga attccaatac gccggccgtg gttgatccca ccaatcccgt
      181 ccccgggagc tattcccgcg tggtcgcgga agttgacgcg cacggccgct ttcgtttcac
      241 gaaaaccaat gcctatagcg gcaacaatgg ccgggccgcg attctgaatg acaaaaacgg
      301 cgcccgcatt gtctacaccg tcggcaatgc cggcaacggt gcaaatcctc agccggatgg
      361 catcattgtc ggcgcgggcg cgcaaatctt gaccgccgag cggacgccgc tggctgcgca
      421 gaatccgggc ctgccgacac cggtcggcag cttcaacatc acagaactgg gactgaaggc
      481 cgacaagatc ggcaaggaca ccaacttccg cggtctcacc atcttcaaca acgtcgtcta
      541 tacgaccaag ggcagcggca gcaatggtat caacacggtc tatttcatag ataccaccgg
      601 gttcgatgcc aacggcaagc cgctggcctg cccgaatggc gtcggtctgc cgggagcaac
      661 cgcgacgctt cccaccgcgc cgattttcta cgacgccacg aaactgcaga cgctgggtgt
      721 tgtgccctac aacatgtgca tcctgaaggg ttttccgacc acgctggcca aaacggcgac
      781 gtcgttcccg ttcggggtct ggtttgccga cgccaagacg ctctatgtcg ccgacgaagg
      841 caatggcacc aacacctttg acccggcatc gggtaagtac acggcggccg ccacgcagac
      901 cggggcgggc ctgcagaagt gggtgttcga cacggggctc ggcgcgtgga acttggccta
      961 tgtgctgcag gccgggctga atctcggcgt gccctacacg gtgggcaatt atcccaccgg
     1021 gcaaaatccc aagaccggcc tgccgtgggc gcccgccgcc gatggccttc gtaacattgt
     1081 cggtcgcgtc aatcgcgatg gtaccgcgac gatctgggcc atcacctcga ccgtcagcgg
     1141 aaacggcgac cagggcgccg atccgaacca gttggtcatg atcaccgatc atctggcagc
     1201 gaccagcgct tccgcaggcg aaagttttgc gacaatccgg accgcgcgat tcggcgaagt
     1261 tcttcgtggc gtgtcgttca cgccgggcac cgatcgcgac cgacttgccg acgcggacga
     1321 ccatcgggcc gatgcggacg atcaccgtgc cgacagggac gacaggcact aatataagtt
     1381 catgccgtga ccagccagcc ggcgccaaca gcgccggctg gcttttgacg tcgatcgttg
     1441 ctgccgccgc gagataccgc gcggacttgt ttcagtccgc gcgctccagc gtcagccgca
     1501 tgccgtcata ggccgggatc acggtcggcg gcaggctctg acgcagcacc tcgtaatcaa
     1561 gatcggagtg catgttggtg atgacggcac gcctcggctt gaagcggtcg atccatgaca
     1621 gcgcgtcgtt gacgctgaag tggctgggat gcccggcata gcgcagcccg tcgatgatcc
     1681 agagatcgag attcttgaga taaggccagc tctcgggcgg gatgtcgctc gcgtcgggag
     1741 tgtaggcggc atcgccgatc cgataaccga gggctggaat attgccgtgc tgcaggatga
     1801 aagcggaaag cgtcatcgca ccgcccttcc cttcgatggt gcggctttcg ccggcttcga
     1861 ttgggtgatt gtccaggatc ggcggatagt cgctgcccgg cggcgccacg aagcagtatg
     1921 aaaaccgcag caggacatga ttcgcggtcg attggttcag atagaccgga atgcgccggc
     1981 gctgatgcag cacgaccgag cggagatcgt cgatgccgtg ggtctgatcg gcatgctcat
     2041 gcgtcagaaa caccgcatcg atgtgatcga cattggcgtc gatcaactgc tcgcgcagat
     2101 cgggcgcggt atcgatcacg atccgcgtca ccccgttgtc cgatgtccgc tcggcaagca
     2161 gcgagcagcg gcggcggcgg tttttcggat tgccgggatc gcaggcaccc cagccgagcg
     2221 ccgggcgcgg caccccggcg gaggatccgc aaccgagtat agtcaggctc agcgtcatgc
     2281 catcgctttc gcggacggca ctttggagaa caggcgaaag aaattttcgg tggtttgacg
     2341 cgatatctcc tcgagcgaaa cgccgcgggt ttccgccagt accttggcaa cttcaaccac
     2401 gtaagacggt tcgttgcgct tgccacgaaa tttgccgggc gccagatacg gcgaatcggt
     2461 ctcaaccatg atgcgatcgg ccgggagttc ggccgcgaga tcgcgcaaac cttgcgactt
     2521 cttgaaggtc agaatgcccg tgaaggaaat cgagaggccc aaggagatcg ctttcatcgc
     2581 cagctcgcgt ccgccggtat agcaatgcaa caccgcgcga aacgatcctt ttgtcatttc
     2641 atcctcgagg acgcggccgc agtcgtcgtc ggcgtcgcgg gtatggataa ccagcggcag
     2701 gccggttttt cgcgcggcgg caatgtgcgc acgaaagccg cgttcctggg cctcgcgtga
     2761 gccatgctgg tagaaattat ccagtcccgc ctcgcccagc gccacgactt ttggatgctt
     2821 cgtcagctcg accagttcgt cggcggggat cccgtcttct tcgtcggcgt gatgcgggtg
     2881 ggtgccgacc gagcaataga catcgggaaa tcgctccgcg atatcaagca attcgcgcag
     2941 acgcttcacc cgcgtcgaga tggtgacgat gcgcccgatg ccggcggccg cggccctcgc
     3001 cacgatcgcg tcgaggtcat cggcgaagtc gggaaaatcg agatggcagt gactatcgac
     3061 gagcattgtt tttaccaacc gttcaggccg cggtcggttc gatgtagcgc ggaaagattc
     3121 cgaccggcgc cggcagcctt gtgcccggct cgatgcgggt cgcgccaccc aggctcgcga
     3181 aatcgcgcgc atccgcgggg atgccgaggc tatccagcaa tcttgctgac gactccggca
     3241 tgac
//
LOCUS       DASWGM010000188         4730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1536133,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000188 DASWGM010000000
VERSION     DASWGM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536133"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VGA15_05750"
     CDS             complement(<1..1019)
                     /locus_tag="VGA15_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX9587232.1"
                     /translation="MQLASQTGDPDQKMPPSIYAALIDSLFQDPGPLFAGALCAAVGA
                     IMTAVKTGNPWLWPCVALLVVTGAVRALDTRQYRQRNSGLTSNEAARWEVRYQFGAML
                     YAAALGLWCVVTLLGSSDAVAHMICTSVTLCYVAAGGGRTYGRPWIFHVQMLLACGPL
                     TLALALYGSPYYFGMAVLNVLFFLALKHISTSLQKIFVRALVAREREAALASQFDTAL
                     NNMPHGLCMFRADGRLAVVNHRFTAMMNLCDDLVHSGAGAPDIISACVSAGSLSDASG
                     RMILSEIGNSQAKDIITTDSSSASGRSVAWTFQPMAGGGTVVLVEDITERKNAEARIS
                     HLARY"
     gene            complement(1201..2487)
                     /gene="tolB"
                     /locus_tag="VGA15_05755"
     CDS             complement(1201..2487)
                     /gene="tolB"
                     /locus_tag="VGA15_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX9587233.1"
                     /translation="MIRPCGLAAQGQQQQRLEIVPGGTQAIPIAIPNFAAGTPSDGEA
                     GVNVAQVITNNLRRSGLFAPIDQAAYIEKTINIDAAPQFANWKTINAQALVTGRITRQ
                     PDGRLKAEFRLWDVTTGQQLTGQQYFTSPEYWRRIAHIISDQIYEHLIGDKGYFDSRV
                     VFVDESGPKERRVKKLALMDQDGANVRYLTKGADLVLTPRFSPARDEQLITYMEFGQG
                     DPRVYLYRIETGEREIVGNFPGMSFSPRFSPDGQRVIMSLQQGGNSNLFVMDLRSKST
                     TRLTDTPAIDTSPSYSPDGTRLCFESDRGGKPQIYVMPATGGAAQRISFGEGSYSTPV
                     WSPRGDYIAFTKQGSGQFSIGIMKTDGSGERILTSGFHNEGPTFAPNGRVLMFFRDPG
                     GNGGPSLFTVDVSGRNEQRVPTPGFASDPAWSPLLS"
     gene            complement(2588..3520)
                     /locus_tag="VGA15_05760"
     CDS             complement(2588..3520)
                     /locus_tag="VGA15_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolA"
                     /protein_id="HEX9587234.1"
                     /translation="MKVDKTLVASVALHVVVLGWGLVSFSSKAFESTEIESVPVEFVT
                     DDELSKITAGIKSGKKENPKPLVEKVAEAKPMDDAVGKITEKKPVVTDAAPDPTPKPV
                     EKPVEKTPDPPKPVAETKPKDEPKPIEKKPDPPKVDPIADALKKEEAKKPAPKPEAKA
                     APPPPVKKREYKFDRESISALVDKQAPSRQAFAGDALNSTAALGTAKGTAATLSASYL
                     SALVSRIHDCWQRDGGGFDEDDLKIPITLTFKVDGTLSSAPLIEIEPRNIRERAIFEG
                     ARRAIIACQPYTMLPQAKYAEWKVLPFLFHNKAF"
     gene            complement(3523..3972)
                     /gene="tolR"
                     /locus_tag="VGA15_05765"
     CDS             complement(3523..3972)
                     /gene="tolR"
                     /locus_tag="VGA15_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX9587235.1"
                     /translation="MAMNMAGSSGGGGRRGRRKAVMAEINVTPMVDVMLVLLIIFMVS
                     APLLTVGVPLDLPQTQAKSLDQDKTPLQLSVDVQGKVFINNTEVAMNELIPKLKAITD
                     ARGGMDERIFMRADKKADYGTVARVMGQLSGAGFKRLALVTEVEQGS"
     gene            complement(4012..4725)
                     /gene="tolQ"
                     /locus_tag="VGA15_05770"
     CDS             complement(4012..4725)
                     /gene="tolQ"
                     /locus_tag="VGA15_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX9587236.1"
                     /translation="MNPADVAQTALPLASTDVSLIALFFQAHWIVKAVMLGLLACSVW
                     VWAIAIDKTFLYARTKRAMDRFEQAFWSGQSIEELYRALSAKPTQSMAACFVAAMREW
                     KRSFESQARSFAGLQMRIEKVMNVSIAREIERLERRLLVLATVGSAGPFVGLFGTVWG
                     IMSSFQSIAASKNTSLAVVAPGIAEALFATAIGLIAAIPATIFYNKFTSEVNRQAQRL
                     EGFADEFSAILSRQIDERA"
BASE COUNT          896 a         1481 c         1426 g          927 t
ORIGIN      
        1 tcgtaacgcg cgagatggct gatcctggct tccgcattct tccgctcggt gatgtcttcc
       61 accagcacaa cggtgccgcc gccggccatc ggttgaaacg tccaggccac cgaccggccg
      121 ctcgcggaac tggagtcggt ggtgatgata tccttggcct gtgaattgcc gatttccgag
      181 agaatcatcc ttccgcttgc gtccgagagc gagccggcgc tgacgcaggc cgagataatg
      241 tcgggggcgc cggcgccgct atgcacgagg tcatcgcaca ggttcatcat tgcggtgaag
      301 cgatgattga cgacggcaag ccggccgtcc gcgcgaaaca tgcacaggcc gtgcggcatg
      361 ttgttcaagg cggtgtcgaa ctggctcgcc agtgccgcct cgcgctcgcg cgcaaccagc
      421 gcccgtacga aaatcttctg caggctggtc gagatgtgtt tgagggcgag gaagaacagt
      481 acgttcagga ccgccatccc gaaatagtac ggacttccat acagcgccag cgccagcgtc
      541 agcggcccgc aggccagcag catttgcaca tgaaagatcc acgggcgccc gtaggttcga
      601 ccgcctccgg ccgccacgta gcaaagcgtg acggatgtgc agatcatgtg cgcgacggca
      661 tcgctgctcc ccagcagcgt caccacgcac catagcccaa gcgccgccgc atagagcatc
      721 gcaccaaact ggtagcggac ttcccagcgt gccgcttcgt tcgacgtcag accggaattg
      781 cgctgccgat attgacgcgt gtcgagcgca cgaacggcgc cggtcacaac cagaagtgcg
      841 acgcagggcc agagccatgg gttgccggtc tttacggccg tcatgatcgc cccgaccgcg
      901 gcgcaaagcg cgcccgcaaa cagcggccca ggatcctgga ataacgaatc gatcagcgcc
      961 gcgtagatcg acggcggcat cttctgatcg gggtcgccgg tctggcttgc aagctgcatc
     1021 gggtgtgcgc acgtcctctt gggtgcgcag ttttacctct cgcagatgaa gtcttcctga
     1081 gggaacatcg ttaccgggaa gttgtttttg gtccgggaaa cgaaaatctg cccgacaaat
     1141 cagcaggcta ggcaagcttt gccgacccgg gcaccaaccc cgggaatggc gctgccaacc
     1201 ttaagaaagc aggggcgacc aggccggatc ggacgcgaag ccgggggtgg gaacccgttg
     1261 ttcattacgt ccggaaacgt ccacggtgaa cagcgaaggt ccgccattgc cgccggggtc
     1321 gcggaagaac atcaaaaccc ggccgttcgg tgcgaaggta ggtccctcgt tgtggaatcc
     1381 gctggtgagg atccgctcgc ccgagccgtc ggtcttcatg atgccaatgg aaaactgacc
     1441 gctgccttgc ttggtgaagg cgatatagtc gccccgcggc gaccacaccg gggtggaata
     1501 gctgccttcg ccaaaggaga tgcgttgcgc ggccccgccg gttgccggca tcacatagat
     1561 ctgcggcttt ccgccacggt cggattcgaa acacaggcgg gtcccgtcgg gcgaataaga
     1621 tggcgaggta tcgatcgcgg gggtgtcggt cagacgcgtg gtcgacttcg agcgcagatc
     1681 catcacgaac aggttcgaat tgccgccctg ctgcaggctc atgatcacgc gctggccatc
     1741 cggcgagaac cgcggcgaga acgacatgcc gggaaaattg ccgacgattt cgcgctcacc
     1801 ggtctcgatt cgatacaagt aaacgcgcgg atcgccctgg ccgaattcca tataggtgat
     1861 caactgctcg tcacgcgcgg gcgaaaaccg cggcgtcagc accagatcgg cgcccttggt
     1921 cagatagcga acattggcgc cgtcctgatc catcagcgcc agcttcttga cgcgccgctc
     1981 cttgggcccg gattcgtcga cgaacaccac gcggctgtca aaatagcctt tgtccccgat
     2041 cagatgctcg tagatctggt cggaaatgat gtgcgcgatc ctccgccaat attccggcga
     2101 ggtgaaatat tgctggccgg tgagctgctg gcccgtggtg acgtcccaca agcggaattc
     2161 cgccttcagg cgtccgtcgg gctgccgggt gatgcggccg gtcaccagcg cctgcgcgtt
     2221 gatggtcttc cagttcgcaa attgcggggc ggcgtcgatg ttgatggttt tttcgatata
     2281 ggctgcctga tcgatcggtg cgaacaggcc gctgcgcctg aggttgttgg tgatgacctg
     2341 ggctacatta accccggctt cgccgtccga tggcgttccc gccgcgaaat tcggtatcgc
     2401 gatcgggatt gcctgagtgc cgccgggtac gatttcaagt cgctgctgct gctgcccttg
     2461 cgcggcgagt ccacacggac ggatcattcc cgacaccgcc aatgcgccgg ccgtgaatgt
     2521 gaaagcgcgc cgggacatcg gtgacgacag acccggcgtc ggattgccgc ggaacaaatc
     2581 agacatgcta gaaagccttg ttatggaaca agaagggaag caccttccac tcggcatatt
     2641 tcgcctgcgg cagcatggta tatggctgac aagcaatgat agcccgacgg gccccctcaa
     2701 aaattgcgcg ctcgcggata ttacgcggtt cgatttcgat caacggcgcc gatgaaagcg
     2761 tgccgtcgac tttaaacgta agcgtgatcg gaatcttcaa atcgtcttca tcgaacccgc
     2821 caccgtcccg ctgccagcaa tcatggatcc tgctgacaag cgcggaaagg taacttgccg
     2881 atagcgttgc cgcggttccc ttggccgttc cgagcgccgc ggtcgaattc agcgcgtcgc
     2941 cggcaaacgc ctgtcgggac ggagcctgtt tgtcgaccag cgccgagatg gattccctgt
     3001 cgaatttgta ttcgcgtttc ttgacaggtg gaggcggcgc cgccttggct tccggcttcg
     3061 gtgccggctt cttggcttct tcttttttca gagcgtccgc gatcggatcg accttgggcg
     3121 gatccggctt cttttcgatc ggcttcggtt catccttggg tttggtctcg gcgaccggct
     3181 tcggcggatc gggcgttttc tcgacgggtt tttccaccgg cttcggcgtc ggatcggggg
     3241 cggcgtcggt cacgaccggt tttttttcgg taatcttgcc gacggcgtca tccatcggct
     3301 tggcttcggc gaccttctcc accagcggct tcgggttttc cttcttgccg gatttgatgc
     3361 cggccgtaat cttggacagt tcgtcgtccg tcacgaattc aacgggaacc gactcgatct
     3421 cggtcgactc aaatgccttg gacgagaacg acaccagtcc ccagccgagc acgacgacgt
     3481 gcaaggccac ggacgcaaca agtgtcttgt cgaccttcac cctcaggacc cctgctccac
     3541 ctcggtgacc agcgccagcc gcttgaagcc ggcccccgac aactgcccca tgaccctcgc
     3601 cacggttccg taatccgcct tcttgtccgc ccgcatgaat atacgctcgt ccatgccgcc
     3661 acgggcgtcc gtgatcgcct ttaatttcgg tatcagctcg ttcatcgcca cttcggtatt
     3721 gttgatgaac acctttccct gaacgtcgac tgaaagctgc agcggcgtct tgtcctgatc
     3781 caggctcttg gcctgggtct gcggcagatc gagcggcacg ccgaccgtca gcagcggcgc
     3841 cgacaccatg aagatgatca gcaacaccag cattacgtcc accatcggcg tgacgttgat
     3901 ctccgccatc actgctttgc gccggccgcg gcgaccgccg ccgcccgacg agcctgccat
     3961 gttcatcgcc atgatctgat actcacaatg gggctcatag catatcgcgc ctcacgcccg
     4021 ctcgtcgatc tggcgcgaca ggatcgctga aaattcatcg gcgaagcctt ccagccgctg
     4081 cgcctgccgg ttcacctcgg aggtgaactt gttgtagaag atagtggccg gaatggcggc
     4141 gataaggccg atagccgttg caaacagggc ttccgcgatg cccggggcca ccacggccag
     4201 cgaggtgttt ttcgaggcgg cgatcgactg gaagctcgac atgatgcccc acaccgtgcc
     4261 gaacaggccg acgaacgggc ccgccgaacc gaccgtcgcc agcaccagca ggcggcgttc
     4321 cagccgctcg atttcccggg cgatggagac gttcatgacc ttttcgatcc gcatctgaag
     4381 cccggcgaaa gagcgcgcct ggctttcgaa cgaccgtttc cattcgcgca tcgccgcgac
     4441 gaagcaggcc gccatcgatt gcgtcggctt ggccgatagc gcgcgataaa gttcctcgat
     4501 cgactgcccc gaccagaacg cctgttcgaa gcgatccatg gcgcgcttgg tgcgggcata
     4561 caggaacgtc ttgtcgatcg cgatcgccca tacccagacc gaacaggcca gcaaccccag
     4621 catgaccgct ttcacgatcc agtgagcctg aaagaaaagc gcgatcagcg atacgtcggt
     4681 ggatgccagc ggcaaggctg tctgagccac gtcggccgga ttcataagcg
//
LOCUS       DASWGM010000189         4146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_16849,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000189 DASWGM010000000
VERSION     DASWGM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16849"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGA15_05775"
     CDS             complement(<1..239)
                     /locus_tag="VGA15_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX9587237.1"
                     /translation="MMDELPDDAAPVATRCGFVALIGAPNVGKSTLVNALVGSKVTIV
                     SRKVQTTRALIRGIVIDNNAQIILVDTPGIFLPKR"
     gene            complement(229..1047)
                     /gene="rnc"
                     /locus_tag="VGA15_05780"
     CDS             complement(229..1047)
                     /gene="rnc"
                     /locus_tag="VGA15_05780"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX9587238.1"
                     /translation="MNDETADIQILSSSGEAGHQSETASEPAAAKKKRSKISAKAAAA
                     AIEARIGHKFADATLLATAFTHVSALKSARKRGDSYQRMEFLGDHVLGLIVSDMLYRA
                     FPNADEGELSKRLADLVRKESCADVAKSLGLVDDIKLGAVGAGAGARLRKSVLGDVCE
                     AVIGAVFLDGGYSAAAQFVERNWTERMRKPRRPLRDPKTVLQEWAQGKGLPTPVYREI
                     ERTGPHHDPQFRVAVDLPGLAPAEGLGGSKRAAEKVAASVMIAREGVGGGSNDG"
     gene            complement(1044..1802)
                     /gene="lepB"
                     /locus_tag="VGA15_05785"
     CDS             complement(1044..1802)
                     /gene="lepB"
                     /locus_tag="VGA15_05785"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9587239.1"
                     /translation="MSVTSGTKSESGFGETVRVVIHALLIALVIRTFLFQPFNIPSGS
                     MKATLLVGDYLFVSKYSYGYSHYSIPLSPPLFSGRVFGSEPARGDIVVFRLPKDDTTD
                     YIKRVIGLPGDRIQMKDGLLYINDKPVQRERLSDFVGEDPCGSDATSRVKRWKETLPN
                     GVTYQSLDCVDNGFYDNTSVYTVPPGHFFMMGDNRDNSTDSRVLSAVGYVPFENIVGR
                     AQMIFFSIAEGEHAWMFWRWPTAVRWNRIFSIVR"
     gene            complement(2242..2661)
                     /gene="acpS"
                     /locus_tag="VGA15_05790"
     CDS             complement(2242..2661)
                     /gene="acpS"
                     /locus_tag="VGA15_05790"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX9587240.1"
                     /translation="MILGIGSDLIDIRRVAKVIERHGDRFLDRIFTDAERARAGRRAK
                     SEKMVVATYAKRFAAKEACSKALGTGIRRGVWWRDMGVVNLPGGRPTLRLTGGALARL
                     EALTPPGHEARIDLSITDDWPLAQAFVIISAVNVGKP"
     gene            complement(2658..3455)
                     /locus_tag="VGA15_05795"
     CDS             complement(2658..3455)
                     /locus_tag="VGA15_05795"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX9587241.1"
                     /translation="MPKALPLRLGVNVDHVATLRNARAGRNPDPVRAALVAIAAGADG
                     ITAHLREDRRHIRDEDMARLKAEISKPLNFEMAATEDMLRIALATKPHAACLVPERRE
                     ELTTEGGLDVVGQHNALAPFIARLNDAGVRVSLFIGAEPRQIEMAAKLRAPVIEIHTG
                     AWCDAVVDGHDAKAESEWQRIVAGAALARSAGLEVHAGHGLDYVTAEKIAGLPEIIEL
                     NIGYYMIGEALFTGLGETVRAMRAAMDRGRKMAAGQMPDSGNLAGKT"
     gene            complement(3621..>4146)
                     /locus_tag="VGA15_05800"
     CDS             complement(3621..>4146)
                     /locus_tag="VGA15_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEX9587242.1"
                     /translation="YGAPKKSLAVKLKLKSPPDPARSPSAIPVRGINSDLPVKFAPNG
                     GAVPGDRIIGIVSPGEGITIYPIQSPALKDFEDEPERWLDVRWDVDETTPQRFPARIM
                     IDNVNEPGSLAQVATVIAEHDGNIDNINMSRRSPDFTELTIDLEVYDLKHLSAIIAQL
                     RAKAVVARVERVNG"
BASE COUNT          750 a         1334 c         1270 g          792 t
ORIGIN      
        1 ctccgcttgg gcaggaaaat cccgggcgtg tcgaccagaa tgatctgggc gttgttgtcg
       61 atcacgatgc cgcggatcag cgcgcgggtg gtctgcacct tgcgcgagac gatcgtgacc
      121 ttggagccga caagagcatt gaccagcgtg gatttgccga cattgggggc gccgatcagc
      181 gcgacaaagc cgcagcgcgt cgcgaccggc gcggcatcgt cagggagttc atccatcatt
      241 gctgccgccg ccgacgcctt cgcgcgcgat catcacggac gccgcgacct tttcggcggc
      301 gcgcttgctg ccgccgaggc cctcggccgg cgccagtccc ggcaaatcca cggcgacgcg
      361 aaactgcgga tcgtgatgcg gcccggtgcg ctcgatttcg cgatagaccg gtgtcggcag
      421 ccccttgccc tgcgcccatt cctgcagcac cgtcttggga tcgcgcagcg gccgccgcgg
      481 cttgcgcatc cgctcggtcc agttgcgctc gacgaactgc gccgcagcac tgtagccgcc
      541 gtcgagaaac acagcaccga tcaccgcctc gcagacgtcg ccaagcacgg atttgcgtaa
      601 gcgcgcgccg gcgccggcgc cgaccgcgcc aagcttgatg tcgtcgacca gcccgagcga
      661 cttcgcgaca tccgcgcagc tctccttgcg cacgagatcg gcgagccgct tcgacagttc
      721 gccttcatcg gcattcggga aggcgcgata cagcatgtcg gatacaatta gcccaagcac
      781 atggtcgccg agaaattcca tgcgctggta actgtcgcca cgcttgcggg cggatttcag
      841 cgcggaaaca tgcgtgaagg ccgtcgccag cagggtggcg tcggcgaatt tgtggccgat
      901 ccgcgcctcg atcgccgcag cggcggcctt ggcgctgatc ttgctgcgct ttttcttcgc
      961 cgccgcaggt tcgctcgccg tctcagactg atggcctgct tcgcctgatg acgacaagat
     1021 ctggatatca gctgtttcgt cgttcatcgc acaattgaga atatgcgatt ccagcgcacc
     1081 gcggtgggcc agcgccagaa catccaggcg tgttcgcctt cggcaatcga gaagaaaatc
     1141 atctgggcgc ggccgacgat attctcaaac ggcacgtaac ccaccgccga cagcacccgg
     1201 ctgtcggtcg agttgtccct gttgtcgccc atcatgaaga agtggccggg cggcacggtg
     1261 tagacactgg tgttgtcgta gaaaccgttg tcgacacaat ccagcgactg gtaggtgacg
     1321 ccgttcggca gcgtctcctt ccagcgcttg acccgcgaag tggcatcgga gccgcaaggg
     1381 tcttcgccga cgaaatcgct caggcgctcg cgttgcaccg gcttgtcatt gatgtagagc
     1441 agcccatcct tcatctggat gcggtcgccc ggcaacccga tcacgcgctt gatgtagtcg
     1501 gtggtatcgt ctttgggcaa ccggaacaca acgatgtcgc cccgcgccgg ctcggagccg
     1561 aatacgcgtc ccgaaaacaa aggcggcgac aacggtatag agtagtggct gtagccgtag
     1621 gaatatttcg agacgaacag atagtcgccg accaggagag ttgccttcat cgatcccgag
     1681 gggatgttga agggctggaa caggaaggtg cggatcacga gcgcgatcaa cagggcatga
     1741 atgacgacgc ggacggtttc accaaagccg ctctcagatt ttgtcccgga tgtcacgctc
     1801 atcgcttttc ccaattccct tcgcgaccgc cgtacggtgg cagaacattt tgcgcgcgcg
     1861 aatcgcccct cgcttcgcgc cgacaaaatg gtcttgatgc tgatattgag agcgaatttt
     1921 ggcgcaagcc cgaattcgcc cgactagatg ttccccctag ggtggcttag gtccggaagc
     1981 tccctttagc cctcgctgaa aggctgccaa gcgagattcc ggacctaaac cacactagaa
     2041 tcagagtttt tcggtaccct tggaatccga agtcgccagg aagatgcagc gatataaacg
     2101 aacttcggat caagacgcca agcgttttag acggttgttc gcagccgcgc aatcaacgac
     2161 cgtatcaaaa cttgctaatc cattgatgca tctgatattt ttagtttcgc ggcaaaccgc
     2221 cggagaccgc cttccggggt gtcacggttt gccgacattg actgccgaaa ttatgacaaa
     2281 ggcctgcgcc agcggccagt cgtcggtaat gctcagatcg atccgcgcct cgtgccctgg
     2341 gggcgtcagc gcctcgagcc gggccagcgc cccgcccgtc agccgcaggg ttggacggcc
     2401 acccggcagg ttcaccaccc ccatatcccg ccaccacacg ccacgccgga tgccggtgcc
     2461 gagcgccttg gaacaggctt ccttggcggc aaaccgcttg gcgtaggttg cgaccaccat
     2521 cttctcgctt ttcgcacggc gcccggccct ggcgcgctcg gcgtcggtga aaatgcggtc
     2581 gagaaagcgg tcaccgtggc gctcgatgac tttggcgacg cgacggatgt cgatcaggtc
     2641 ggagccgatg ccgaggatca tgtcttgcct gcaaggttcc cgctgtcggg catttgaccc
     2701 gcggccatct tgcgcccgcg gtccatcgcc gcccgcatcg cgcgaaccgt ctccccgagg
     2761 cccgtgaaca acgcctcccc gatcatgtag tagccgatat tcagttcgat gatctctggt
     2821 aagcctgcga tcttctccgc ggtcacataa tcgagcccgt gaccggcgtg gacttccagc
     2881 ccggccgagc gcgccagcgc cgcgcctgcg acgatccgct gccattccga ttcggccttg
     2941 gcgtcatgac catcgaccac ggcgtcgcac caggcgccgg tgtgaatctc gatcaccggc
     3001 gcacgcagct ttgccgccat ctcgatctgc cgcggctcgg cgccgatgaa cagcgacacc
     3061 cgcaccccgg catcgttcaa tctggcgata aacggcgcca gcgcgttgtg ctgcccgacc
     3121 acgtcgaggc cgccctcggt ggtgagttct tcgcggcgct cgggcaccag gcacgccgca
     3181 tgcggcttgg tcgcgagcgc gatccgcagc atgtcctcgg tggccgccat ctcgaaattc
     3241 agcggcttgg agatctccgc cttcagccgc gccatgtcct cgtcgcggat gtggcggcgg
     3301 tcctcgcgca gatgcgcggt gatgccgtcg gcgccggccg cgatcgcaac aagcgcagcg
     3361 cgcaccggat cgggattgcg gcccgccctc gcgttgcgaa gggtggcgac gtgatcgaca
     3421 ttgactccga ggcgcagcgg aagagctttc ggcataacgg actcgcacta gaatttagag
     3481 ggcggctgaa cactccggct gtcatgcgcg ggctcgaccc gcgcatccat cttcttgaaa
     3541 agaatggatt gccgggagcg tagacaagta tacgtagtct gcgcaaggca gaccactatg
     3601 cccggcaatg acgaacttta ctacccattg acgcgctcga cccgtgcgac cacggccttg
     3661 gcgcgcaact gggcgatgat agcgctgaga tgcttcaggt catagacctc aagatcgata
     3721 gtcagttcgg tgaaatccgg cgagcgtctg ctcatgttga tgttgtcgat attgccgtcg
     3781 tgctcggcga tcacggtcgc cacctgggca aggctgccgg gctcgttgac gttgtcgatc
     3841 atgattcgcg ccggaaagcg ctgcggcgtc gtttcgtcga cgtcccagcg cacgtcgagc
     3901 cagcgttccg gctcgtcttc gaaatccttt aacgccggcg actggatcgg atagatcgtg
     3961 atgccttccc ccggcgaaac gatcccgatg atccggtcgc ccggtaccgc gccgccgttc
     4021 ggcgcgaact tgaccggcaa atcggagttg atgccgcgca ccggtatcgc cgacgggctg
     4081 cgcgcggggt cgggcggcga tttcagcttg agtttgaccg ccaggctctt cttgggggcg
     4141 ccgtat
//
LOCUS       DASWGM010000190         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2234909,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000190 DASWGM010000000
VERSION     DASWGM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234909"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGA15_05805"
     CDS             <1..182
                     /locus_tag="VGA15_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX9587243.1"
                     /translation="LPIDYIEKRNAIVDAVTLDDAKKAAKRLWGQGLLTVIVGRAPQA
                     AAQPAAATPPPPKAN"
     gene            458..877
                     /gene="arfB"
                     /locus_tag="VGA15_05810"
     CDS             458..877
                     /gene="arfB"
                     /locus_tag="VGA15_05810"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX9587244.1"
                     /translation="MLRISRDLTIDENDIEISFVRASGPGGQNVNKLSTAAQLRFDTR
                     RLTLPADAEARLNRLAGQRMTKDGVIVIHAQRFRTQERNRADAVDRLLELLRAAMTRP
                     VPRRPTRPTMGSKKRRLEGKKRRSDIKAKRGTARFDD"
     gene            1117..1401
                     /locus_tag="VGA15_05815"
     CDS             1117..1401
                     /locus_tag="VGA15_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9587245.1"
                     /translation="MLFKQQCATCHTTNISDPPRQGPTLFNIVGRDAGTADGFRYSAG
                     FANAKFKWDELKLDAWLADPQRMIPGAIMPYKQAKPELRMAIVSYLKELH"
     gene            1402..1797
                     /locus_tag="VGA15_05820"
     CDS             1402..1797
                     /locus_tag="VGA15_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9587246.1"
                     /translation="MAKPVHSMIRVLDEARSLDFYARAFGLEVADHLKFADFALIYLR
                     HPSSPFEVELTVNFDRKAAYELGDGYGHLAVVVDDLDAEHARFSREKLSPGPLRDFKH
                     DGATLARFFFVSDPDGYKIEVIQKGGRFG"
     gene            1831..2379
                     /locus_tag="VGA15_05825"
     CDS             1831..2379
                     /locus_tag="VGA15_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX9587247.1"
                     /translation="MREVDRRSKYSRRTFLQGAAAVAPVAAVATSAGLGIEDAWAADA
                     TTLAPATLKTLVKVARDIYPHDFLVDSYYITAIKPWDGKAAKDPATKALLEDGVRRLD
                     QDAQDRHKVAYAQVPWEADRVVLLQGIEQTDFFKKVRSDLVVSLYNQEELWPKFGYEG
                     SSAEHGGYIKRGFNDIDWLPKV"
BASE COUNT          480 a          812 c          728 g          394 t
ORIGIN      
        1 agctgcccat cgactacatc gagaagcgca acgccatcgt cgatgccgtg accctcgacg
       61 atgcgaaaaa ggccgccaag cggctgtggg ggcaaggcct cctcaccgtg atcgtcggcc
      121 gcgccccgca agccgccgcc cagccggcgg ctgcgacccc gccgccaccc aaggcgaact
      181 gacggctttc ggcttatctc gtgtcccgga cgcggtgcag cgcctcttgg cggtgcgccg
      241 cagagccggg acccagcttt ctttgccaac catggacccc ggattcagca gcgcaccacg
      301 ccgcaagaca tagcgcgtcg aagacgcgcg tgaacgcgct gatggcgctg cgcagcatcc
      361 ggggaacgcc tccctcgtgc ttcagcgcca caaacaaaac cgctatcctg cgcctgtccg
      421 attcccgttc ggaatcatct ccgcttccgg tgagcccatg ctgcggattt cccgcgacct
      481 cacgatcgac gagaacgaca tcgagatcag tttcgtccgc gcctccggtc cgggcggaca
      541 gaacgtcaac aagctctcga ccgcagccca actgcggttc gatacgaggc ggctgacgct
      601 gccggcggat gccgaggccc ggctcaaccg gctcgccggc cagcgcatga ccaaggacgg
      661 cgtgatcgtg atccatgcgc agcgtttccg cacccaggag cgcaaccgcg ccgacgccgt
      721 cgaccgcctg ctcgagcttt tgcgcgccgc gatgacgcgc cccgtcccgc ggcgtccgac
      781 caggcccaca atgggctcga aaaagcgccg gctcgaaggc aaaaagcgcc gcagtgacat
      841 caaggcaaag cgcggcacgg cgagattcga cgactaggac gttttcgagc gaagtggcga
      901 ccggccggca gccgcgcttt gttccgccct gcaacagccc actactacca ccgcccggtc
      961 gccgcggctt aaggtccgct cgcgccaacg gtgatggaca tgacaaaacg acgcgcggca
     1021 ctgcggatcg ggctggcatt ttgcgccgcg gcgctggctg tcgaccgcgc ggcggcgcaa
     1081 ctaaccccgc ctgccgcaaa gccagccgat ggcgcgatgc tgttcaagca gcaatgcgcc
     1141 acttgccaca ccaccaacat ctccgatcca ccgcggcagg gaccgacgct gttcaacatc
     1201 gtcggacgcg acgccggaac cgccgacggc ttccgctatt cggcgggttt tgccaatgca
     1261 aaattcaaat gggatgagct caagctcgac gcctggctgg ccgatccgca acgaatgatc
     1321 cccggcgcga tcatgcctta caagcaggcc aaacccgaac tccgcatggc gatcgtgagc
     1381 tatctgaagg agttgcactg aatggcgaaa ccagttcact cgatgattcg cgtgctcgat
     1441 gaagcaaggt cgcttgattt ctacgcgcgc gctttcgggc tcgaggtcgc cgaccatctc
     1501 aaatttgcgg actttgcgct gatctatctg cgtcatccgt cctcgccgtt cgaggtcgaa
     1561 ctgacggtga atttcgatcg caaggcggct tacgaattgg gcgacggcta cggccacctc
     1621 gccgtcgtgg tcgacgatct cgatgccgag catgcccgct tcagccgcga aaaactgtca
     1681 cccgggccgc tgcgcgattt caagcatgac ggcgcgacgc tggcgcggtt tttcttcgtc
     1741 tccgaccccg acggctacaa gatcgaagtg atccagaaag gcggacgctt cggataatca
     1801 cgtcaacaaa aacgttcaat ggaggatccc atgagagaag tcgatcgtcg aagcaaatac
     1861 agtcgccgaa cctttctgca gggcgccgca gccgtagcgc cggtcgcggc cgtcgccacc
     1921 agcgccggcc tcggcatcga ggatgcgtgg gctgcggacg cgaccacgct ggcgccggcg
     1981 acgctgaaaa cgctggtcaa ggtcgcgcgc gatatctatc cgcacgactt cctggtcgac
     2041 agctactaca tcaccgcgat caagccgtgg gacggcaagg cggcgaaaga tcccgccacc
     2101 aaggcgctgc ttgaagacgg cgttcgtcgt ctcgatcagg acgcgcagga ccgccacaag
     2161 gtggcctatg cgcaggtgcc gtgggaagcc gaccgcgtcg tgctgctgca gggcatcgag
     2221 cagaccgact ttttcaagaa ggttcgctcc gacctggtcg tctcgctcta caaccaggaa
     2281 gagctgtggc cgaagttcgg ctacgagggc tcctccgccg agcatggcgg ctacatcaag
     2341 cgcggtttca acgacatcga ctggctgccg aaggtctgag gacaacttcg cgcagaacat
     2401 cagttgggag gata
//
LOCUS       DASWGM010000191         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2510323,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000191 DASWGM010000000
VERSION     DASWGM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510323"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VGA15_05830"
     CDS             complement(<1..660)
                     /locus_tag="VGA15_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9587248.1"
                     /translation="MSRLMLLVCQLLVAVVVLGLWQLLATVPVFGRILLPPFFFSNPL
                     DVGSQIVDWFASGVIWKHLAITLWESILAFAIGSIAGVAVGFWFARQPRVAAVFDPYV
                     KMANALPRVVLAPIFTLWLGLGIWSKVALGVTLVFFIVFFNVYQGVKEVSTTVLDNGR
                     MLGMNERQLTRHVYWPSALSWMFSSLHTSVGFAVVGAVVGEYLGSAAGLGYLIQQAEG
                     VF"
     gene            complement(899..1732)
                     /locus_tag="VGA15_05835"
     CDS             complement(899..1732)
                     /locus_tag="VGA15_05835"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587249.1"
                     /translation="MQAGETAGSTAVALDDATVAFPVADGRVYTAVEKARLWIEHGEF
                     VAIVGPTGCGKSTLLNVAAGLLRPASGTVRIFDAPLTGLNRQAGYLFQADALFPWKTA
                     IDNVAIGLEISGAPRGHALERAQGWLTAVGLGGFGNRYPHMLSGGQRKRVGLAQVLIR
                     DPKILLMDEPFGPLDAQTRQIMGNLLLELWNADRKAVLFVTHDLEEAIALADRVVIMS
                     AGPSARIIGDWRVPLARPRDISEIRMEKDFHALHREIWSVLKDEVMKGYVQSSQAAEA
                     G"
BASE COUNT          390 a          666 c          588 g          309 t
ORIGIN      
        1 gaacacgcct tcggcctgct ggatcagata gccgagcccc gccgccgagc cgagatattc
       61 gccgaccacg gcgccgacca cggcgaagcc gaccgaggta tgcagcgagg agaacatcca
      121 cgacagcgcc gacggccagt agacatgtcg cgtcaactgc cgttcgttca tgccgagcat
      181 gcggccatta tccagcacgg tggtgctgac ctccttgacg ccctgataga cgttgaagaa
      241 cacgatgaag aacaccagcg tgacgccgag cgcgaccttc gaccagatgc caaggccaag
      301 ccacagcgtg aagatgggcg ccagaacgac gcgcggcagc gcgttggcca tcttgacgta
      361 gggatcgaat accgcggcaa cccggggctg gcgcgcgaac cagaaaccga cggcgacccc
      421 ggcaatcgag ccgatcgcga aagcgagaat tgactcccac agcgtgatcg cgaggtgttt
      481 ccagatcacg ccgcttgcga accagtcgac aatctggcta ccgacgtcga gcggattgga
      541 aaagaagaac ggcggcagca atatgcggcc aaagaccggc accgtggcga gcaactgcca
      601 cagccccagc acgacgacgg cgaccaacaa ttgacagacg agaagcatca ggcgggacat
      661 ggcgatgctt ctctcgactc ccctccccct tgcggcaggg ttgcggcctc gcacaagctc
      721 tcagcaggtc gtcccggcga acgccgggac ccataacccc tggcgttgcc ctttgcagaa
      781 ggcgtctgcc ccaatgccga aacgcgactc ctcgccgtcg cgatgagcgc aatcgcgctc
      841 atcgctgggg tcccggcgtt cgccgggacg acgaatttcg tcaatgcaga tggcgccatc
      901 agcccgcctc cgccgcctgc gacgattgca catacccctt catcacttcg tccttcagca
      961 cgctccagat ttcccggtgc agcgcgtgaa aatccttctc catgcggatt tccgaaatgt
     1021 cgcgcgggcg ggccagcggc acgcgccagt cgccgatgat gcgcgcactg ggcccggccg
     1081 acatgatcac gacacggtcg gcgagcgcga tcgcctcttc gagatcgtgg gtgacgaaca
     1141 gcaccgcctt gcggtcggca ttccacaatt ccagcaagag attgcccatg atctgtcgcg
     1201 tctgggcatc gagcggcccg aacggctcgt ccatcagcag gatcttggga tcgcggatca
     1261 acacttgcgc gagcccgacg cgcttgcgct gtccgcccga cagcatatgc ggatagcgat
     1321 tgccgaaccc gccgagaccc acggcggtga gccagccctg cgcccgttcc agcgcgtggc
     1381 cgcgcggcgc gcccgaaatc tcgagaccaa tggcgacgtt gtcgatcgcg gtcttccagg
     1441 gaaacagcgc atcggcctgg aacagatagc cggcctgccg attgaggccc gtcagcggag
     1501 catcgaaaat ccgcaccgtt cccgatgccg gtctgagcag gccggcggcg acattgagca
     1561 gcgtcgattt cccgcacccg gtgggcccga cgatggcgac gaactcgcca tgctcgatcc
     1621 agagccgagc cttttcgacc gcggtataga cacggccatc tgcaactggg aacgccaccg
     1681 tcgcgtcgtc aagcgcgacc gccgtcgatc ctgcggtctc gcctgcctgc acccgcttcc
     1741 cccctgttgt ggcctgtgga ttaccgccat catcccaaca attcatccga tcggggcaat
     1801 aggcaaggcc cgcgtcggct tggtgggttg gtcgcggtgt gatagtccca acgttatgag
     1861 caccgccccg ccactgatcg catttgcgca tgtcggcaaa agcttcgacg gcggccgcgg
     1921 cgcgaaaccc gttctggccg tgaacgacgt ctc
//
LOCUS       DASWGM010000192         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2093872,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000192 DASWGM010000000
VERSION     DASWGM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093872"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGA15_05840"
     CDS             complement(<1..192)
                     /locus_tag="VGA15_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HcpA family protein"
                     /protein_id="HEX9587250.1"
                     /translation="MKILRPILILASCLTFAANAAAQISLVPPGVDTPPADKPAAKAK
                     AKPAAAAKKPAVPVATPNPP"
     gene            complement(201..881)
                     /locus_tag="VGA15_05845"
     CDS             complement(201..881)
                     /locus_tag="VGA15_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX9587251.1"
                     /translation="MANKPVPPRPVPRLYLATPVVDDPALLMASLPGLLAAADVAAVL
                     LRLKPTDQRTMISRIKTLAPVIQDGGAALLLDGHVELVARAGADGAHLTGIEAMEDAM
                     PTLKPDRIAGVGGLATRHDSMAAGEAGADYVLFGEPDAKGQRPSVEAIAERLQWWAEL
                     FEPPCVGFAASREEAHEFAAAGADFVLVGDFIWNDKRGAKAALMEAEQTIRQAHAAMS
                     ATARAEQG"
     gene            complement(884..1927)
                     /locus_tag="VGA15_05850"
     CDS             complement(884..1927)
                     /locus_tag="VGA15_05850"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX9587252.1"
                     /translation="MNLADLNKIALAMVAPGKGLLAADESSGTIKKRFDDINVVSTEE
                     SRRDYREMLFRSSEAMSRCISGVILYDETIWQNAKDGTPLVKIVEQSGAIPGIKVDEG
                     TQALPGCPGELITAGLDKLAERLKKYYDRGARFAKWRAVIDIGSGTGGRNIPTMTAIH
                     VNAHALARYAALCQAAQIVPIVEPEVLMDGDHDIDRCYEVTQRVLNKTFQELRIQRVA
                     LEGMILKPNMAVSGKKSAKPASVEEVAEKTIRLLKACVPAAVPGIAFLSGGQTDQDAT
                     AHLDAMNRIGGLPWRLTFSYGRALQAAPQKAWAGKAENVAAGQRALTHRARMNSLASK
                     GEWKADLEQQKAA"
     gene            complement(2113..>2449)
                     /locus_tag="VGA15_05855"
     CDS             complement(2113..>2449)
                     /locus_tag="VGA15_05855"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX9587253.1"
                     /translation="INANGGKMKPTWGVPVSEIQRGIKNGVRKINIDTDNRMAMTGQI
                     RKVLNEDPSEFDPRKYLKPAMEAMAKLCKQRLQEFNTAGQASKIKKVLTTAEMAKRYV
                     KGELDPKVA"
BASE COUNT          412 a          793 c          751 g          493 t
ORIGIN      
        1 cggcggattt ggcgtcgcga cgggaactgc cggcttcttg gccgccgccg ctggcttcgc
       61 tttcgccttg gcggctggtt tgtccgccgg cggcgtgtcg accccgggag gcaccagcga
      121 gatttgcgcc gccgcgttcg ccgcgaatgt cagacagctt gcaaggatca atatcggacg
      181 caggatcttc atttccagcg ttatccctgt tcggctctgg ctgttgcgga catcgcggcg
      241 tgcgcctgtc ggatggtctg ctccgcctcc atcaaggctg ccttggcacc gcgcttgtca
      301 ttccagatga aatcgccgac cagcacgaaa tcagcaccag ccgcggcaaa ttcgtgggcc
      361 tcttcgcgcg acgccgcaaa gccgacgcac ggcggctcga acagctcagc ccaccattgc
      421 agtcgctcgg cgatcgcctc gaccgacggc cgctgccctt tcgcatcggg ctcgccgaac
      481 agcacgtagt ccgcgccggc ttcgcccgcg gccatcgaat cgtgccgcgt cgccagtcca
      541 ccgacgccgg cgatgcggtc gggttttaac gtcggcatgg catcctccat ggcttcgatc
      601 ccggtcagat gcgcgccatc ggcgccggcg cgggccacca gttcgacatg gccatcgagc
      661 aggagcgcag caccgccgtc ctggatcaca ggcgccaggg ttttgatgcg cgagatcatg
      721 gtgcgctggt cggtcggctt gagccgcagc agcaccgccg cgacatcggc cgcggcgagc
      781 aggcccggca ggctcgccat caacagggcc ggatcgtcca ccaccggcgt cgccagatag
      841 agccgcggca cggggcgcgg cggaacaggt ttattggcca aatctatgcc gccttctgct
      901 gttccagatc ggccttccat tcgcctttgc tcgcgagcga gttcatccgc gcgcggtggg
      961 tcaatgcgcg ctggcccgcg gcgacgttct cggccttgcc cgcccacgcc ttctgcggcg
     1021 ccgcctgcag tgcgcgaccg taggagaagg tgagacgcca cggcaggccg ccgatccggt
     1081 tcatggcgtc aagatgcgcg gtggcgtcct ggtcggtctg cccgccggag agaaaggcaa
     1141 tgccgggcac cgccgcaggc acgcaagctt tcagcagccg aatggttttc tccgcgacct
     1201 cttcgacgga agccggtttc gcgcttttct tgccggagac ggccatgttg ggtttcagga
     1261 tcatgccttc cagcgcgacc cgctgaatcc gcaattcctg aaacgtcttg ttgagaacgc
     1321 gctgggtcac ttcatagcag cggtcgatat catgatcacc atccatcaag acttccggct
     1381 cgacgatcgg aacgatctgc gcggcctggc acaatgcggc atatcgcgcg agcgcgtggg
     1441 cattgacgtg gatcgcggtc atggtcggaa tgttccggcc gccagtgccg ctgccgatat
     1501 cgatcaccgc gcgccatttt gcgaaccgcg cgccgcggtc gtaatatttc ttcagacgct
     1561 cggcgagctt gtccagcccg gcggtgatca attcacccgg gcagcccggc agagcttgcg
     1621 tgccctcatc gaccttgatg ccggggatcg cgcctgattg ttcaacgatc ttcaccagcg
     1681 gcgtgccatc cttggcattt tgccagatcg tctcgtcata gagaatgacg cccgagatgc
     1741 agcggctcat cgcttcgctg gagcggaaca gcatctcgcg atagtcgcgg cgcgactcct
     1801 cggttgatac gacgttgatg tcgtcgaagc gcttcttgat cgtgcctgag gactcgtcgg
     1861 cggcgagcag gcccttgccc ggtgcgacca tggccaaagc gattttgttg agatcggcga
     1921 gattcatcga tgcctcctcc gaaagcactg tgtccttgca agtcaaagtg gacggttctc
     1981 aggcaaaccc ccaagaatac gccaataaga aaaacaccaa tagaaaacgc caataggaaa
     2041 gcgccaataa gaaaacgcca ataaacgtgc gcgggcgccg ccgaggccgg ctcgctcagg
     2101 agtggccgcc tgtcacgcca ctttcggatc gagttcgcct ttgacataac gcttcgccat
     2161 ctcggcggtg gtcagcacct tcttgatctt gctggcctga cccgcggtgt tgaattcctg
     2221 caggcgctgt ttgcaaagtt tcgccatcgc ctccatcgcg ggcttgagat atttgcgcgg
     2281 atcgaattcg ctgggatcct cgttgagtac cttgcggatc tggccggtca tcgccatccg
     2341 gttgtcggtg tcgatgttga tcttgcggac gccgttcttg atgccgcgct ggatctcgga
     2401 aaccggcacg ccccaggtcg gcttcatctt gccgccattg gcgttgatg
//
LOCUS       DASWGM010000193         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2510390,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000193 DASWGM010000000
VERSION     DASWGM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510390"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VGA15_05860"
     CDS             <1..637
                     /locus_tag="VGA15_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587254.1"
                     /translation="TYKAEWYVKTGGQLETFAQGHSVEVAAEHYADIPALKGVHEATI
                     AAALEDALAATRQAPGPEPRIVTPKQEAAIRLDPGGNDLPGTLAREAIVPFLNGAQDL
                     WLAGCANFYDSPFAAKGDACPSPFWSCLDCSNAVITARKLPALIAFLAFTITQRESLA
                     EADWEAKFGRAHRRIRDQILPAFPAAVVAAARMEAAAAPGIIYLPPEASAT"
     gene            652..1002
                     /locus_tag="VGA15_05865"
     CDS             652..1002
                     /locus_tag="VGA15_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587255.1"
                     /translation="MPSPDTWGAVSRLRRDDELVLATQALKPGTDETSLSRFGDDRWD
                     LAPAVFRENAPHALTVVDFAMLDDPMQRLTAKEFVWARLNEPSPCPTRARMAPTVARA
                     TLTDLSSFMQFVAR"
     gene            1018..>2346
                     /locus_tag="VGA15_05870"
     CDS             1018..>2346
                     /locus_tag="VGA15_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587256.1"
                     /translation="MNLVDQAMLDAYLAELRRERRRTAERVAHLIDVPIELDRYSPFL
                     TLGGFGCRPWRGRSAARVAGLPPRPMVAENRTPRIPEPVIAALLRWSLKYIDLFAADI
                     FAARVDLNRLEQVAHLPDDAATRPPLIDRLDVYIGRRCADGRGIPISSLDRRGRRTQS
                     QSTGMLEPAINFDLIGLQLGCDKLLLISRPALRGRLERAIERLVGEIGGMDSTIAIDP
                     DTSLAWRERFDQRSIAEEERMLQAAAYVVCAYLSGMRDSELQAMRVGCWSLSRSADGI
                     VERHRIKSITYKAREISGEEAEWVTIAPVARAIEVVERLSVQQRETRGVDTIWQTLAM
                     NNGNMTVLRGGVIHLINGLRDHLDTRSPEGPAIPHVEGSPWWFTPRQFRRTLAWYIAN
                     RPFGTVAGKIQYKHASIAMFEGYSGSSPSGFRREIEQERALGQIDDVVEHY"
BASE COUNT          429 a          758 c          729 g          430 t
ORIGIN      
        1 gacctacaag gccgagtggt atgtgaagac gggtggccag cttgagacgt tcgcccaagg
       61 gcactccgtc gaagtggcgg cagagcacta tgccgacatt cccgcgctca agggcgtgca
      121 tgaagcaacg atcgcggctg cactcgagga tgcactggcg gcaacgcgcc aggcgcctgg
      181 tccagaacca cgcatcgtca cgcccaagca agaggcggcg atccgccttg atcctggcgg
      241 caacgatctg cccggaacac tcgcacgcga agcgattgta cctttcctca atggagcgca
      301 ggacctgtgg ctcgcgggct gcgccaactt ctacgacagt ccgttcgcag caaagggcga
      361 cgcctgtcca tcgccgttct ggagctgcct cgactgcagc aatgccgtca tcactgcccg
      421 caagcttccg gcgctgatcg ccttccttgc atttacaatt acgcagcggg agtcgctggc
      481 tgaagccgat tgggaagcca agttcggtcg tgctcaccgg cgcatccgcg atcagatcct
      541 gccagccttc ccggcggcgg tggtcgccgc tgcgcgcatg gaggccgcag cagcaccagg
      601 catcatctac ctgccgccgg aagcgagcgc gacttgacgg caattcaacc catgccatcg
      661 cccgatacct ggggcgccgt ttcgcggttg cgacgtgacg acgaactcgt gctggcgacg
      721 caggcgctca agccgggaac ggatgagacc agcttgtcgc gctttggcga cgaccgctgg
      781 gatctcgctc ccgcggtctt ccgcgagaac gcaccgcatg cgctcacagt cgtcgacttt
      841 gcgatgctcg acgatcccat gcagcgcttg accgccaagg agttcgtctg ggcgcggctg
      901 aacgagcctt caccctgccc gacgcgggct cgcatggcgc cgacggtcgc ccgcgcaacg
      961 ctgacggacc taagctcctt catgcagttc gtcgcgcggt aggccggctc gttcgcaatg
     1021 aacctggtcg atcaagcaat gctcgacgcc tatctcgctg agcttcggcg cgagcggagg
     1081 cgaaccgccg aacgcgtcgc tcatctgatc gacgtaccga tcgaactcga tcgctattca
     1141 ccattcctga cgctcggcgg ctttggttgc cgtccttggc gaggccgttc cgcggcgcgg
     1201 gtcgccggac tgccgccgcg accgatggtg gcagagaacc gcacgccgcg catcccggaa
     1261 ccagtcatcg ctgcgttgtt gcgatggtcg ctgaagtata tcgatctctt cgccgccgat
     1321 attttcgcag cccgcgttga tctcaatcgg ctggaacagg tcgcacatct gcccgacgac
     1381 gccgcgacac gccctccgct cattgatcgg ctcgatgtct atatcggccg gcgttgtgcg
     1441 gacggtcgcg gcattcccat ttccagcctc gaccggcgcg gtcggcgcac acaaagccag
     1501 tcgaccggca tgctggagcc cgccatcaac tttgatctga tcggccttca actcggctgc
     1561 gacaagctcc tcctgatttc tcgcccagcg ctccgcggac gactggagcg tgccattgaa
     1621 cgtcttgtcg gcgagatcgg cggcatggac agcaccatcg caattgatcc ggacacgagc
     1681 ttggcatggc gcgagcggtt cgatcaacgc agtatcgccg aagaagagcg gatgctgcag
     1741 gccgcagcct atgtcgtctg cgcctacctg agtggtatgc gcgatagcga actgcaggcg
     1801 atgcgcgtcg gctgctggtc gctcagccga agcgctgacg ggatcgtcga acggcatcgt
     1861 atcaagagca tcacctataa agcgcgggag ataagcggcg aagaagccga gtgggtaacc
     1921 atcgcacctg tggcgcgcgc gatcgaggtg gtggagcgcc tttccgtgca acaacgggag
     1981 acccgcggcg tcgataccat ctggcagacg ttggcgatga acaatggcaa catgaccgtg
     2041 cttcgcggag gtgtgatcca tcttatcaat ggcctgcgcg accatctcga cacgcgttcg
     2101 cccgagggtc cggcgattcc gcacgtcgag ggtagcccgt ggtggtttac accccgtcag
     2161 tttcgaagga cgctcgcctg gtacatcgcc aatcgaccgt tcggtacggt ggcgggcaag
     2221 attcagtaca agcatgcctc gatcgccatg ttcgaggggt attccggctc cagtccatcg
     2281 ggctttcgcc gcgagatcga gcaggaacgc gcgctcggcc agatcgacga tgtggtcgag
     2341 cattac
//
LOCUS       DASWGM010000194        13281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1954331,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000194 DASWGM010000000
VERSION     DASWGM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954331"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..928
                     /gene="hpaD"
                     /locus_tag="VGA15_05875"
     CDS             5..928
                     /gene="hpaD"
                     /locus_tag="VGA15_05875"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HEX9587257.1"
                     /translation="MLDVVDLNASREFYENTVGLHVEDRDDRAVYLRGSEEHQHHSLV
                     LRKAAAAACNRLSFKVGNETDLDKAAAFFSENGIAYAFAKLPFQGRTLQFTDPFGFQI
                     ELYATMEKRPHLLRRYDLYKGCHPQRLDHFNVFAAEVQDTVDFYARLGFRLTEYGEED
                     GPNGRIAAAWMHRKGNVHDLAITNGKGPRLHHFAYWVPTAMNILHLCDVMASSGYLKN
                     LERGPGRHGISNAFFLYIRDPDGHRLELYTSDYFTGDHDHEPLRWSLRDPRRQTLWGA
                     PAPRSWFEQGSPFTGQAIREPLFVADVTIAD"
     gene            1177..1887
                     /gene="modA"
                     /locus_tag="VGA15_05880"
     CDS             1177..1887
                     /gene="modA"
                     /locus_tag="VGA15_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9587258.1"
                     /translation="MSSITNLKAAELKVIAGGSMTGSLNELGPQFERASGHKLVIHFN
                     STPNIIALVTSGTPFDLAVVPVDVFKDAGAKARFAAGPTIDIARVGYGVIVRAGTPKP
                     DISTSDALKKTLLDAPSVAFVPASAAGAYVLKVFDRLGIAEAMKAKTKPQTTPADIAP
                     AVAKGEADLGVFLVNVLMAPGVEPAGSFPAELQQDLVFTAAVAAETKEADAARAFIDY
                     LTTPAATAVIRAKGMTPG"
     gene            1942..2814
                     /locus_tag="VGA15_05885"
     CDS             1942..2814
                     /locus_tag="VGA15_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9587259.1"
                     /translation="MTSHRLATFTVNGSTKYGAVVEGGLVDLSARFAKDYPTLREAIA
                     AGALTRLAEDAAQRSPDHALDAITWLPPIPSPEKIICIGVNYPDRNAEYKDGQDAPKY
                     PSMFMRTPRSFVGHNTPLVRPRASAQLDYEGEVVLIIGKAGRHIPESTALDHIAAVTL
                     CNEGTIRDWVRHAKFNVTQGKNFDSTGSLGPWLVPYTDEKQIADIRLTTRVNGETRQD
                     DRTGRLIFGFRYLINYISTFTTLVAGDVIVTGTPTGAGARFDPPRYLKPGDVVEVEAE
                     NIGMLRNGVIDEAS"
     gene            2843..4468
                     /locus_tag="VGA15_05890"
     CDS             2843..4468
                     /locus_tag="VGA15_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9587260.1"
                     /translation="MTVCTGGEAIVSGLVAHGVDTVFGLPGAQIYGLFDAFQQAQLKV
                     IGARHEQACGYMAYGYARSTGRPGVFSVVPGPGVLNAGAALLTAFGSNEPVLCLTGQV
                     PTQYLGKGRGHLHEMPDQLATLRSFVKWADRIEYPDAAPTLVSRAFQEMLSGRRGPAA
                     LEMPWDVFTQRADTGPAKPFDAFPAPQPDTERIKAAAALIAGSKTPMIFVGSGAIRAR
                     DEILELAELIDAPVVAFRSGRGIVSNAHELGLTMAAAYRMWSHTDLMIGIGSRMELPT
                     MFRWPFRPDGLKSVRIDIDASEMRRFTPDAPVIADAQAGTRELLAAVRKAGYTKTSGR
                     RAAIRDASASALREIQKVQPQMAYLNILREVLPPNAIVTDELSQVGFASWYGFPVYEP
                     RTFITSGYQGTLGSGFPTALGAKVANPGRPVVAITGDGGFMFGVQELATAVQFNIGVV
                     TLVFNNNAYGNVRRDQRERFDGRVVAADLVNPDFVKLAESFGVDAARVTSPGTFRPAL
                     EKALANGGPYLIDIEVPRDSEVSPWAFIHPAKP"
     gene            complement(4580..5233)
                     /gene="alkB"
                     /locus_tag="VGA15_05895"
     CDS             complement(4580..5233)
                     /gene="alkB"
                     /locus_tag="VGA15_05895"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEX9587261.1"
                     /translation="MTVDLFAGVPDVRPSQEAMAERAVLLRGFALALEADLIADLRAV
                     VEQAPFRHMVTPGGHQMSVAMTNCGSVGWVTDRTGYRYDGIDPESGKPWPAMPASFRA
                     LAGQAAAQAGFDGFSPEACLINRYQPGARMSLHQDKDETDFAAPIVSVSLGLPAIFLF
                     GGLKRSDRPRRFRLEHGDIAVWGGPTRLAFHGVAPLADGEHALMGRQRINLTFRKVR"
     gene            complement(5245..5994)
                     /locus_tag="VGA15_05900"
     CDS             complement(5245..5994)
                     /locus_tag="VGA15_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX9587262.1"
                     /translation="MKSIAKNIIRPAPRSDIAARVDAIDWTQAGRDLNAQGCAVLKGL
                     LSVDECRALAALYPDDSRFRSRVVMGRHGFGRGEYKYFSYPLPDIIAELRPALYAQLH
                     GVANSWNEAMGIDIRYPRSHDAFLKRCHAAGQTRPTPLLLQYGVDDYNCLHQDLYGEH
                     VFPLQVAILLSEPGRDFTGGEFVLTEQRPRMQSRPEVVPLAQGDAVAFAVHVRPVQGT
                     RGFYRVNLRHGVSRIRSGHRHTIGVIFHDAK"
     gene            complement(6190..6876)
                     /locus_tag="VGA15_05905"
     CDS             complement(6190..6876)
                     /locus_tag="VGA15_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HEX9587263.1"
                     /translation="MFDLTFTIDAQGTTTPLTLAIDQAVVAGWTGRDPVARDKHIAEL
                     EAIGIARPASTPIYYRVSARRLTTEDSIEVSGTESSGEVEFVLIGWQGRIFVGAASDH
                     TDRKVETYSVTVSKQMCDKPIAPVLWELEEVIGHWDQMILRSHAWIDGARVLYQEGTL
                     DSMLPVADLIQRGFGDGRLPDGCAMFGGTFAAKGGIRPASRFEFELEDPVLRRSIRHG
                     YDVIELPVLG"
     gene            complement(6869..8218)
                     /locus_tag="VGA15_05910"
     CDS             complement(6869..8218)
                     /locus_tag="VGA15_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9587264.1"
                     /translation="MPNIPTLATLADDLQGGRTSARKLVDECLARIADTSGEGMRTFI
                     HVDAEAAIEAAEAMDRLREVKAAPSRYAGIPVSIKDLFDIKGQVTRAGSRALEDSAPA
                     EADASVVARLRRAGFVVIGRTNMTEFAFSGIGINPHFGTPKGAWQRHVGHVPGGSSSG
                     AAVSVVDGMAHGALGTDTGGSCRIPAAFNGIVGFKPTQRRVPLDGGVPLSSTLDSFGP
                     LARTAQCCAVLDAVLADEPVRPLQPRPIKGMRLAVPTTIVLDELEDAVARTFERALET
                     LSRQGALIERIEVPEFLDVGVMNTKGGFAAAESYAWHRYLIVSHGDVYDPRVSKRILR
                     GEAISAADYIDLIGARRSLIARAEKRIAPYDALILPTTANTPPRISDLADDKAFAAQN
                     LLALRNCTLINMIDGCAISLPCHREGEVPVGLTLAGAGGSDRRIFELAAAIEGAIRV"
     gene            complement(8249..8569)
                     /locus_tag="VGA15_05915"
     CDS             complement(8249..8569)
                     /locus_tag="VGA15_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587265.1"
                     /translation="MKLREIAHSRTGDKGNTSNISVIAYHEKHYPLLLAQVTSARVRA
                     HFAGVVEGEVVRYELPNIAALNFVMAGALGGGVTRSLALDAHGKSLSSALLDLEIEAA
                     SDHE"
     gene            complement(8573..9913)
                     /locus_tag="VGA15_05920"
     CDS             complement(8573..9913)
                     /locus_tag="VGA15_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX9587266.1"
                     /translation="MRTIRIGSGAGYSGDRIEPAVELAENGDIQYLVFECLGERTVAL
                     AQQARMKNPDGGYDPLLEERMRAVLPVCAAKGIKIVTNMGAANSVAAARKTAEIARSL
                     GLASLKIAAVIGDDVLDACKERDLPIMEFEGTIKQLGNRLLSANAYLGAEPIADALTG
                     GADIVITGRASDPALFLAPMIHAFGWAMDDWNLLGQGTVAGHLLECAGQITGGYFADP
                     GYKDVLDLARLGFPIGEVGEDGSLVITKVEGSGGVVTARTCKEQLLYEVHDPRRYIQP
                     DVVADFSQVKVEEIAPDRVRISGGRGTERTDTLKVSVGYVDSFIGEGQISYAGPGALA
                     RGRLALDIVRERLKLTGVAASELRFELIGVDALHGAEISARANEPYEVRVRVAGRTEN
                     LREAIRIGNEVETLYTNGPAAGGGAFKSARDVVAVASVLLPRELAKPTIRFVGA"
     gene            complement(9913..10632)
                     /locus_tag="VGA15_05925"
     CDS             complement(9913..10632)
                     /locus_tag="VGA15_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587267.1"
                     /translation="MPPEAAALMEAKGVDAGYGTMQVLWRVDLDVRAGETVLLLGANG
                     AGKTTFLKSLVGLIEARHGSIQLAGEDVTLMRSSDRMRLGMTYMSELAVFPDLSIEEN
                     IRVGAQALGHADPGVRVEELYGVFPALREKRRAPASSLSGGQRKMLGIAKALAAEPRL
                     LVMDEPSAGLSPLFVKEVIRILSDLRGRGLALLIAEQNIGFLEVATRVFVLEGGRIRF
                     SGTVAEMTDNEALRRAYFGLK"
     gene            complement(10622..11353)
                     /locus_tag="VGA15_05930"
     CDS             complement(10622..11353)
                     /locus_tag="VGA15_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587268.1"
                     /translation="MSLDRAPILNVTKLTKRFGGFYALDGLSFHVASGEILGLVGPNG
                     SGKTTAINVISGLYAPDGGEVVLDGADIGGIASHKLVHRGINRTFQIPKPFLSLTVRE
                     NIAVALAYGRAAVAPPAMAELLDEYRLNEVADRPAADLNNAQQKMLDLVRALATRPRL
                     LLLDELAAGLNPAELDWIAERIKALARSGIAIIVVEHLMGFIEHITDRVIVMNAGKEI
                     FEGKLAVAVKVPQVIEVFLGGEHAA"
     gene            complement(11343..12263)
                     /locus_tag="VGA15_05935"
     CDS             complement(11343..12263)
                     /locus_tag="VGA15_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587269.1"
                     /translation="MPSAFRHVLFIVVPLLAIFAVLPGVYQNHLLLFNFVIFLILAQG
                     VNIIYGFTGYLPFGYVGFFGAGAYGFAIMVMHFQAPAILAVLVAGIIGVALGLLLTPL
                     LRLSGAYFAIANLAASLAVLHFVANPALENITRGPYGVSLTGTFNPTLAYAAALVVLA
                     LTLSAVVFLKNSRFGLALQAVREDAVSASMAGVDVVRMRVIAWLASALVAGLAGGVYA
                     WYVSVFYPDNVFSGEFSIFAIVFALFGGVATITGPIVGVIILYGIYNLIGFTTPQYFQ
                     LIYGLLIMGLVLFLPAGLVSLATRRGWHVP"
     gene            complement(12256..>13281)
                     /locus_tag="VGA15_05940"
     CDS             complement(12256..>13281)
                     /locus_tag="VGA15_05940"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587270.1"
                     /translation="SRSIRTKPPPASRSIRVHDAIADDAPDARSGALMLTYALVAGVL
                     FGLYFSLVGIGLNLVFGVMRIVNLAHGDFLMLGAFVAFGVVTLAGIDPLFAVPLAFVI
                     FVVIGLLLYWVLVPRLQGSVNPEMLSIILFFGLSQVIEAVTTIFFGTSERSIQSRALG
                     TVFSTIRIKLFGGKPDAAGPIQIFGQGFPAAWVIAAITSLIAISLVYVYLYRTRLGTL
                     TRAVMSRRDEAFATGIDVDRVSAAAFGIGLGLAAVAGVFAPFMFGSVTPAFGADATVT
                     SFAIVVLGSLGNPLGTALGGVVYGVCYMLVQTYLSSWADLLPYVLLIFILLLRPSGLL
                     GRQVRVA"
BASE COUNT         2625 a         4342 c         4037 g         2277 t
ORIGIN      
        1 cgtcgtgctc gacgtggtgg acctgaacgc cagccgggag ttttatgaaa acaccgtcgg
       61 actgcatgtc gaggatcgcg acgaccgcgc ggtgtacttg cgcggcagcg aggaacatca
      121 gcatcactcg ctggtgctgc gcaaggcggc agccgccgcc tgcaaccgcc tgagcttcaa
      181 ggtcggcaat gaaaccgatc tcgacaaggc cgcggcattc ttttccgaga acggcatcgc
      241 ttacgcattc gccaagctgc cgttccaagg tcgcacgctg cagttcaccg atcccttcgg
      301 cttccagatc gagctctatg cgaccatgga gaagcgcccg catttgttgc gccgctacga
      361 tctctacaag ggctgccacc cgcagcggct cgaccacttc aatgtgttcg ccgcggaggt
      421 tcaggacacc gtcgacttct atgcgcggtt gggttttcga ctgaccgaat acggcgagga
      481 agacggcccg aacgggcgta tcgcggcggc gtggatgcat cgcaagggca atgtgcatga
      541 tcttgctatc accaatggca agggcccccg cctgcaccat ttcgcgtatt gggtgccgac
      601 cgcgatgaac attttgcatc tgtgcgacgt gatggcctcg agcggttacc tcaagaacct
      661 cgagcgcggg cccggccgcc acggcatttc aaatgcgttt tttctttata tccgcgaccc
      721 cgacggccat cgcctggaac tctacaccag cgattatttc accggggatc acgaccacga
      781 accgttgcgc tggtcgctca gggatccgcg gcggcaaacg ctatggggcg cgcccgcgcc
      841 gcgctcgtgg ttcgaacagg gctcgccatt tacgggacag gcgatacgcg aaccgctgtt
      901 tgtcgccgat gtcacgattg ccgattagag tcgattgatg aattctcgcc gcgatgtacg
      961 attttccgat agcgaaggtt ctccccgcgc gtcgtcccgg cgcacgccgg gacccataac
     1021 ccgcggcgac gatgtttcag aaggtgtctg cctgagtgcc ctaatgttac aacacggcgt
     1081 atgggtcccg gcgtgcgccg ggacggcaca aattgtatcg gacacccgat gactcctctc
     1141 gtggcagtca cttcgataat gagcttggtg ctttccatgt cgtcgatcac aaacctgaag
     1201 gccgctgaac tcaaggtcat cgccggtggt tcgatgacgg gctcgctgaa tgaactcggc
     1261 cctcagttcg agcgcgcctc cggccacaag cttgtcattc atttcaattc caccccgaac
     1321 atcatcgcgc tggttacatc gggcacgccg ttcgacctcg ctgtcgttcc cgtcgatgtt
     1381 ttcaaggacg ccggcgcgaa ggcccgcttc gccgcgggcc ccaccatcga tatcgcgcgc
     1441 gtcggttatg gcgtcatcgt ccgcgccgga acgcccaagc ccgacatcag cacatcggac
     1501 gccttgaaga agaccctgct tgatgcgcca tcggttgcct tcgttccggc aagtgctgcc
     1561 ggcgcttatg tcctgaaggt gttcgaccgt ctcggcattg ccgaggcgat gaaggccaag
     1621 accaagcctc agaccacacc ggccgacatc gctccggccg tcgccaaagg cgaggccgat
     1681 ctcggggtgt ttctcgtcaa cgtgttgatg gcccccggtg tcgaacccgc cggttcgttc
     1741 cccgccgagt tgcagcagga tctggttttc acggcagccg tcgcagcgga gaccaaggaa
     1801 gcggacgccg ccagggcgtt catcgactat ctgaccactc ccgccgcgac agctgtcatc
     1861 agggccaaag gcatgacgcc cggctgaagc ggccctgcag gccgggcgat agcttcacga
     1921 ccactcgtta ttggacatct catgacctca catcgcctcg caactttcac cgtcaacgga
     1981 tcgaccaaat acggcgccgt ggtcgaaggc gggctagtcg atctctccgc gcgctttgca
     2041 aaagactatc cgacgctccg tgaagcgatt gcggccggcg ccctgacaag actggccgag
     2101 gatgccgcgc agcgttcgcc ggaccacgcg ctcgacgcga tcacctggtt gccacccatc
     2161 ccctcgccgg aaaaaatcat ctgcatcggc gtcaattatc cggatcgcaa cgcggaatat
     2221 aaagacggcc aggacgcccc gaaatatccc agcatgttca tgcgcacgcc gcgctcgttc
     2281 gtcggccaca acacgccgtt ggtccgcccc cgcgcatccg cgcaactcga ctacgaaggc
     2341 gaagtggttt tgatcatcgg caaggccggc cgccacatcc cggagagcac ggcgctcgat
     2401 cacatcgccg cggtcacgct gtgcaacgaa ggcaccatcc gcgattgggt ccggcacgca
     2461 aaattcaacg tcacgcaagg caagaatttc gattccaccg gcagcctcgg cccgtggctg
     2521 gttccctaca ccgatgaaaa gcagatcgcg gacatcaggc tgaccacgcg ggtcaacggc
     2581 gagacgcggc aggacgaccg taccggccgg ctgatcttcg gctttcgcta tctcatcaac
     2641 tacatctcga ccttcaccac gctggtagca ggtgacgtca ttgttacggg aacgccgacc
     2701 ggcgccggtg cgcggttcga tccgccgcgc tatctgaagc ccggcgatgt cgtcgaagtg
     2761 gaggccgaaa acatcggcat gctgcgcaac ggcgtgatcg acgaagcctc gtgatttcct
     2821 ttgcaagcga ttggataatg caatgacagt gtgtaccggc ggcgaagcca tcgtcagcgg
     2881 cctcgtcgcg catggcgtcg ataccgtgtt cggcctgccc ggcgcccaga tctacgggct
     2941 gttcgacgcg ttccagcagg cacagctgaa ggtgatcggc gcccgccacg agcaggcttg
     3001 cggctacatg gcctatggct atgcgcgatc caccggaagg cccggcgtgt tcagcgtggt
     3061 gcccggcccg ggcgtgctca atgcgggtgc agctctgctg accgccttcg gctcgaacga
     3121 accggtgctc tgcctgaccg gacaggtgcc gacgcaatat ctcggcaagg gccgcggcca
     3181 tctgcacgaa atgccggatc agctcgcgac acttcgaagt ttcgtgaaat gggccgaccg
     3241 catcgaatat cccgacgcag caccgaccct tgtcagccgc gcgtttcagg aaatgctgtc
     3301 ggggcggcgc ggccccgcgg cgctggaaat gccctgggat gtgttcacgc agcgcgcaga
     3361 caccggcccg gcaaaaccgt ttgatgcttt tccggcgccg cagcccgata ccgaacgcat
     3421 caaggccgcc gccgcgctga tcgcaggcag caagacgccg atgatttttg tcggcagtgg
     3481 cgctattcgc gcgcgtgacg aaatcctcga acttgcggaa ctgatcgacg cgccggtggt
     3541 ggcgttccgc agcggccgcg gcatcgtcag caatgcgcac gagctcggat tgacgatggc
     3601 ggcggcctat cggatgtggt cgcacaccga tctgatgatc gggatcggtt cgcgcatgga
     3661 attgccgacc atgttccgct ggccgtttcg ccccgatggc ctgaaatctg ttcgtatcga
     3721 tatcgatgct tcggaaatgc gccgcttcac gccggatgct cctgtcattg ccgacgcgca
     3781 ggccggcacg cgtgaactgc tcgcggcggt gagaaaggcc ggctacacga aaaccagcgg
     3841 ccgccgcgcc gcgatccgcg acgcctcggc ctcggcgctg cgagagatcc agaaggttca
     3901 gccgcagatg gcttacctga acattctgcg cgaggtattg ccgccaaacg ctatcgtcac
     3961 cgacgaattg tcgcaggtcg gctttgcctc ctggtacggt tttccggtct acgagccgcg
     4021 taccttcatc acatcaggct atcagggcac gctcggttcc ggctttccca ccgcgctcgg
     4081 cgccaaggtg gcgaatccgg gccggccggt ggtcgccatc accggcgacg gcggcttcat
     4141 gttcggcgtg caggaactgg ccacggctgt gcagttcaac atcggcgtgg tgacgctggt
     4201 gttcaacaac aacgcctatg gcaatgttcg gcgcgatcag cgcgagcgtt ttgacggccg
     4261 cgtggtggcc gccgatctgg tcaatccgga ttttgtgaaa ctcgcggaat cctttggcgt
     4321 cgatgctgcg cgcgtaacct cacccgggac gtttcggccc gcgctggaaa aagcgctggc
     4381 gaacggcggg ccttacctca tcgacatcga agtgcccagg gattccgagg tcagcccgtg
     4441 ggcgttcatt catccggcga agccgtaaac tacatactgt cattccgggg cgacgcgaag
     4501 cgtcgaaccc ggaatctcga gattccgggt tcgcgctacg cgcgccccgg aatgacgtag
     4561 caaaataaat tcggcagccc taccgcacct tccggaacgt caggttgatc cgctgccggc
     4621 ccatcagggc atgttcgccg tccgcaagcg gcgccacgcc gtgaaacgcc agccgcgtcg
     4681 ggccgcccca caccgcgatg tcgccgtgct cgagccgaaa ccggcgcggc cggtcgctgc
     4741 gcttcaatcc gccgaacagg aagatcgcgg gcaggcccag cgagaccgag acgatcggcg
     4801 cggcgaaatc ggtctcgtcc ttgtcctgat gcaaggacat ccgcgctccc ggctggtagc
     4861 ggttgatcag gcacgcttcc ggggaaaacc cgtcaaaccc tgcctgcgcg gccgcctgcc
     4921 cggcaagcgc gcgaaacgat gccggcatcg ccggccaggg ttttccggat tcaggatcga
     4981 tgccgtcgta gcgataaccg gtgcgatcgg tgacccaccc cacgctgccg caatttgtca
     5041 tcgccaccga catctggtgg ccgccgggtg tcaccatgtg gcgaaacggg gcttgctcca
     5101 cgacagcgcg caagtcagcg atcagatcgg cttcgagcgc caacgcaaaa ccgcgtagca
     5161 gcacggcgcg ctccgccatc gcctcctgcg acgggcgaac atcgggaacg ccggcaaata
     5221 aatccacagt caaatgcctc gcactcactt cgcatcgtga aagatcacgc caatggtgtg
     5281 gcgatggccg gagcggatcc ggctgacgcc atgacgcagg ttaacgcgat agaatccgcg
     5341 cgtcccctgc accggcctaa catgcacagc aaaagccacg gcatcgccct gggcgagcgg
     5401 caccacttcg ggccgcgact gcatgcgcgg ccgctgctcg gtcaggacga attcgccgcc
     5461 ggtaaaatcc cgacccggct ccgacaacag gatcgcgacc tgcagcggga aaacatgctc
     5521 gccatagaga tcctgatgca ggcagttgta gtcgtcgacg ccatattgca gcagcagcgg
     5581 tgtcggccgg gtctggccgg cagcatggca tcgcttcagg aacgcatcat gactccgggg
     5641 atagcggata tcgatgccca tggcctcgtt ccagctgtta gcgacgccgt gcagttgcgc
     5701 gtacagcgcg ggacgcaatt ccgcgattat gtcgggcagg ggataggaga aatatttgta
     5761 ttcgccgcgc ccgaacccat gccgtcccat caccacccgg ctgcggaacc gactgtcgtc
     5821 cggataaagg gcagccagcg cgcggcattc atcgaccgag agcagcccct tcaggaccgc
     5881 gcagccctgc gcattgagat cgcggccggc ctgggtccag tcgatggcat caacgcgcgc
     5941 ggcgatatcg gaacgcggcg cgggacggat gatgttcttt gcgattgatt tcatggcgat
     6001 caatgtcgcg aaccgagcag tcccgcgcca cccgatttcc gggcaaattg gcaatggagc
     6061 tcgatgattg cagcgtgaag ctttaagccg tcatggccgg gctccgcccc ggccatccac
     6121 gtcccatgat atccgccgat ctgaagacgt ggatgcccga cgcaggtcgg gcatgacgat
     6181 ttaaacaatt cagccgagca caggaagttc aatgacatcg tagccgtggc gaatgctgcg
     6241 cctgagaacg ggatcttcga gttcaaactc aaagcggctc gccggccgga tgccgccctt
     6301 ggccgcaaaa gtgccgccga acatggcgca gccgtccggt agccgaccat cgccaaagcc
     6361 gcgctgaatc aggtccgcca ccgggagcat gctgtcgagt gtaccttcct ggtacagtac
     6421 ccgcgcaccg tcgatccagg catgcgaacg caggatcatt tgatcccagt gaccgatgac
     6481 ttcctcaagt tcccacagca cgggcgcgat cggcttgtcg cacatctgct tggagaccgt
     6541 gacgctatag gtttccacct tgcggtcggt atggtcggag gcggcgccga caaagatgcg
     6601 gccctgccag ccgatcagca cgaactcgac ctcgccgctc gattccgtgc cgctgacttc
     6661 gatgctgtct tcggtggtca accgccgcgc ggagacgcga taatagatcg gcgtggacgc
     6721 aggccgcgca atgccgatcg cttcgagttc ggcgatgtgc ttgtcgcgcg ccaccggatc
     6781 gcggccggtc cagccggcga cgacggcctg atcgatcgcc agtgtcagcg gcgtggtcgt
     6841 gccctgggcg tcgatggtga aggtaagatc aaacacggat ggcaccttct attgcagcgg
     6901 cgagttcgaa gatgcgccga tccgatccgc ctgcgcccgc gagcgtcagc ccgaccggga
     6961 cctcgccctc gcgatggcag ggcagcgaaa tggcgcagcc gtcgatcatg ttgatcaacg
     7021 tacaattgcg cagcgccagc aggttttgcg cggcaaacgc cttgtcgtcg gccagatccg
     7081 agatgcgcgg cggcgtgttg gcggtggtcg gcaagatcag cgcatcatag ggtgcaatac
     7141 gtttttccgc gcgcgcgatc agcgaccgcc gcgcgccgat caaatcgata tagtccgcgg
     7201 cgctaatggc ctcgccgcga aggatacgtt tgcttacgcg gggatcgtag acatcgccat
     7261 ggctgacgat cagatagcga tgccaggcat aactttccgc cgccgcaaaa ccgcccttgg
     7321 tgttcatcac cccgacatcg aggaattccg gcacttcgat ccgctcgatc agcgcgccct
     7381 gccgcgacag cgtctccagc gcacgctcga atgtccgcgc caccgcatcc tcgagttcgt
     7441 cgagcacgat cgtggtcgga accgccagac gcatcccctt gattggacgc ggctgcagtg
     7501 gccttaccgg ctcgtcggcg agtacggcgt cgagcaccgc acagcactga gccgtgcgcg
     7561 ccagcggccc aaagctgtcg agcgtggacg acagcggcac accgccatcg agcggcaccc
     7621 ggcgctgggt gggcttgaac ccgacaatgc cgttgaaggc cgccggaatc cggcacgagc
     7681 cgccggtgtc ggtgccgagc gcgccatgcg ccatgccatc gacgacggat accgcggccc
     7741 ccgatgacga tccgccgggt acgtgcccca catggcgctg ccatgcgccc ttcggcgtgc
     7801 cgaaatgcgg attgatgccg atgccggaaa acgcgaattc ggtcatgttg gtgcggccga
     7861 tcacgacgaa gccggcacgg cgcagccgcg ccaccaccga tgcatcggct tccgccggag
     7921 ctgaatcctc cagcgcacgc gaaccggcgc gggtcacctg ccccttgatg tcgaacagat
     7981 ccttgatcga gacgggaatg ccggcgtagc gcgacggcgc cgccttcacc tcgcgcagcc
     8041 ggtccatcgc ttcggctgct tcgatcgcgg cttccgcatc gacatgaatg aaggtccgca
     8101 tcccctcgcc tgaggtgtcg gcgatcctgg cgagacattc gtcgaccagt ttgcgggcgc
     8161 tggtgcggcc gccttggagg tcatcagcca gcgtggccag cgtcggaata tttggcatcg
     8221 tactttcgcc tcatggctgc agaatcgatt attcgtggtc cgaagcggcc tcgatttcca
     8281 gatcgagcag cgccgagctc agggatttgc catgggcatc gagcgccaac gaccgcgtca
     8341 ccccgccgcc gagcgcgccg gccatgacaa agttcagcgc ggcaatgttg ggcagctcat
     8401 agcggacaac ctcgccttcg acaacaccgg caaaatgcgc tcttacccgc gcgctggtga
     8461 cttgcgcgag cagcagcgga taatgcttct cgtgataagc aatcaccgag atattcgatg
     8521 tgttgccctt atcgccggtg cgggaatggg cgatctcgcg cagcttcacg tctcacgctc
     8581 ccacaaaacg gattgtcggt ttggcaagtt cgcgcggcag caggactgaa gcgaccgcga
     8641 cgacgtcgcg cgccgatttg aaagcgccgc cgccggcggc ggggccattg gtgtagagcg
     8701 tttcgacctc gttgccgatt ctgatcgcct cgcgcaggtt ttcggtccgg ccggcgacgc
     8761 ggacgcgcac ctcatagggt tcattcgcgc gtgcggagat ttccgccccg tgcagcgcgt
     8821 cgacgccgat cagttcgaac cgcaattcgc tcgctgcaac gccggtgagt ttcagccgct
     8881 cgcgaacgat gtccagcgcc agccgcccgc gcgccagcgc gccgggaccg gcataggaaa
     8941 tctgcccctc gccaataaaa ctgtcgacat agccgaccga gactttcagc gtatcggtac
     9001 gctcagtccc gcgcccgccg ctgatccgca cgcggtctgg tgcgatctcc tcgaccttca
     9061 cttgcgaaaa gtccgccacc acgtcgggct ggatgtagcg cctgggatcg tgcacctcgt
     9121 agagcagttg ctccttgcag gtccgcgccg tcaccacacc gccggagcct tcgaccttgg
     9181 tgatgacgag cgagccgtct tcacccactt cgccgatcgg aaagccgagc cgcgccaaat
     9241 ccaaaacatc cttgtagccg ggatcggcga aatagccgcc ggtgatctgg cccgcgcatt
     9301 ccagcaaatg cccggcgacg gtgccctgcc ccagcagatt ccagtcgtcc atcgcccagc
     9361 caaaggcgtg aatcatcggc gccagaaaca gcgcgggatc cgacgcccgc ccggtgatca
     9421 cgatatccgc gccgccggtc agcgcgtcgg cgatcggttc ggcgccgaga taggcattgg
     9481 ccgacaatag ccggttgccg agctgtttga tcgttccctc gaactccatg atcggcagat
     9541 cacgctcttt gcaggcgtcc agcacgtcat cgccgatgac ggcggcgatc ttcagcgacg
     9601 cgagaccgag cgatctggca atctcggcgg tcttgcgggc cgcagcgacc gaattggccg
     9661 cgcccatatt ggtgacgatc ttgattccct tggcggcaca gaccggcagc accgcgcgca
     9721 tccgctcctc gagcagcggg tcgtatccgc catcagggtt tttcatgcgc gcctgctgcg
     9781 ccaacgcgac ggtgcgctcg ccgagacact cgaacaccag atactggata tcgccgtttt
     9841 cggcgagctc gaccgcaggc tcgatgcgat cgcccgaata gccggcaccg gatcctatcc
     9901 taatcgttcg catcatttca gcccgaaata agcccgccgc agcgcttcgt tatcggtcat
     9961 ttccgcgacc gtgccggaaa agcggatgcg gccgccttcc agcacaaaca cccgcgtcgc
    10021 cacttcgagg aaaccaatat tctgctcggc gatcaagagc gccagcccgc ggccgcgcaa
    10081 atcgctgagg atccggatga cctccttgac gaacaggggc gacagcccgg ccgagggttc
    10141 atccatcacc aacagccgcg gctcggccgc aagcgccttg gcgatgccga gcatcttgcg
    10201 ctggccgccg gagagactgg acgccggcgc ccggcgtttt tcgcgcagcg ccggaaatac
    10261 gccgtagagt tcttcgacac ggacgcctgg atcggcatga cccagcgcct gcgcgccgac
    10321 gcggatgttt tcctcgatcg acaaatcggg aaataccgcg agttcggaca tataggtcat
    10381 gccgagccgc atccggtcgc tggagcgcat cagcgtcacg tcctcgccgg caagctggat
    10441 gctgccgtgc cgcgcctcga tcaggccgac cagcgatttg aggaaagtgg ttttgccggc
    10501 gccattggcg ccgagcagca gcacggtttc gccggcgcgg acgtcgagat cgacgcgcca
    10561 cagcacctgc atcgtgccgt agccggcgtc gacgcctttg gcttccatca gcgctgctgc
    10621 ttcaggcggc atgctcgcct cccaaaaaca cttcgatgac ttgcggcacc ttcacggcga
    10681 ccgcgagctt gccctcaaag atctccttgc cggcgttcat gacaatgacg cggtcggtaa
    10741 tgtgttcgat aaaacccatc agatgctcga ccacgatgat cgcgatcccg gatcgtgcca
    10801 gcgccttgat gcgttcggcg atccagtcca gctccgccgg attgagcccg gcggcgagtt
    10861 cgtcgagcaa cagcagccgc ggacgggtgg cgagcgcgcg aaccagatcg agcatcttct
    10921 gctgcgcgtt gttgagatcg gccgccggcc ggtcggcgac ctcattaagc cgatattcgt
    10981 cgagtaattc cgccatcgcc ggcggggcca ccgcggcgcg cccataggcc agcgcgaccg
    11041 cgatattctc gcgcaccgtc agcgacagaa acggttttgg gatctgaaag gtgcggttga
    11101 tgccgcgatg gaccagtttg tgcgatgcta ttccgccgat atcagcacca tcgagcacca
    11161 cctcgccgcc gtcgggcgca taaagaccgg atatcacgtt gatcgcggta gtcttgccgg
    11221 aaccgttggg gcccaccaat cccagaattt cgccggacgc gacatgaaaa ctcaagccgt
    11281 cgagcgcgta aaacccgccg aagcgctttg tcagctttgt cacgttgaga atgggcgcgc
    11341 gatcaaggga catgccatcc cctccgcgtc gcgagcgaaa ccaggccggc ggggagaaac
    11401 agcaccagcc ccatgatcaa cagcccgtag atcaactgga aatattgcgg cgtggtgaac
    11461 ccaatcagat tgtagatgcc atagaggatg ataacgccga caatcgggcc cgtgatcgtg
    11521 gcgacgccac cgaacagcgc aaacacaatg gcaaaaatgc tgaattcgcc gctgaagacg
    11581 ttgtcgggat agaacaccga gacataccaa gcatagaccc cgccggcgag acccgccacc
    11641 agcgccgacg ccagccacgc gatcacgcgc atccgcacga cgtccacacc tgccatcgac
    11701 gcagagaccg catcttcccg caccgcctgc aacgcgaggc caaagcggga gtttttcagg
    11761 aacacgaccg cggagagcgt cagcgccagc accacaaggg cggcggcata ggccagtgtc
    11821 ggattgaacg tccccgtgag cgaaacgccg tacggcccgc gcgtgatgtt ctccagcgcc
    11881 ggattggcga cgaagtgaag caccgccagc gacgctgcga gattggcgat cgcgaaatag
    11941 gcgccggaaa gacgcagcaa tggcgtcagc aatagcccga gcgcgacgcc gattatcccg
    12001 gcaacgagca ccgcgaggat ggcgggcgcc tgaaagtgca tcaccatgat ggcaaaaccg
    12061 taggcgccgg cgccgaagaa gccgacatag ccgaacggca gatagccggt gaaaccgtag
    12121 atgatgttca cgccctgggc gaggatcaaa aagatcacga aattgaacag cagcagatga
    12181 ttttggtaga cgcccggcag caccgcaaag atggcgagca gcggcacgac gatgaagagc
    12241 acgtgtctga aggcgctagg caacgcgcac ctgccttccg agcagcccgc tcggacggag
    12301 cagcaggatg aaaatcagga gcacataggg caaaaggtca gcccatgaac tcagataagt
    12361 ctgcaccagc atgtagcaga cgccgtaaac gacgccgcca agcgcagtcc ctaagggatt
    12421 gccgagcgat cccagcacca cgatggcgaa ggacgtgacg gtcgcgtcgg cgccgaacgc
    12481 cggcgtcacc gatccgaaca tgaacggcgc gaacaccccg gccaccgccg caagccccaa
    12541 tccgataccg aacgccgccg ccgagacacg atcgacatcg atgccggtcg cgaaggcctc
    12601 gtcgcgccgc gacatcaccg cacgcgtcag ggtgccgagc cgggtgcggt acagatagac
    12661 gtacaccagc gaaatcgcga tcaggctggt gatggcagcg atcacccagg cggcgggaaa
    12721 tccttgccca aagatctgga tcggtccggc cgcgtccggc ttgccgccga acagttttat
    12781 cctgatggtc gagaacacgg tgccgagcgc acggctctgg atcgagcgtt cgctggtgcc
    12841 gaaaaagatc gtcgtgaccg cctcgatcac ctgcgacaac ccgaagaaca ggatgatcga
    12901 cagcatttcc gggttcaccg aaccctgcaa ccgcggcacc agcacccaat agagcagcag
    12961 cccgatcacg acaaagatca cgaacgccag cggcaccgcg aacaagggat cgatgccggc
    13021 caacgtcacg acgccgaacg cgacaaaggc gccgagcatc agaaagtcgc catgcgcaag
    13081 attgacgatg cgcatgacgc cgaacacgag attgaggccg ataccgacca ggctgaagta
    13141 aagaccgaac aggacgccgg cgacgagcgc gtaagttagc atcagcgctc cgctccgggc
    13201 atccggtgca tcatccgcaa tcgcatcatg gacgcggata gaccggcttg ccggtggcgg
    13261 tttcgtgcgg atagatcgag a
//
LOCUS       DASWGM010000195         6605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2374756,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000195 DASWGM010000000
VERSION     DASWGM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374756"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGA15_05945"
     CDS             <1..314
                     /locus_tag="VGA15_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9587271.1"
                     /translation="PIIETAKVDDAFLIPDEEAVLIIYELLEHEGLCVGGSTGINVAG
                     AIQLARQLGPGHTIVTVLCDSGYRSQSKLFNPDFMRSKNLPVPQWLEKRSKIDVPFEK
                     V"
     gene            complement(457..1008)
                     /locus_tag="VGA15_05950"
     CDS             complement(457..1008)
                     /locus_tag="VGA15_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587272.1"
                     /translation="MLGFLFGFNARLGRLHYFLSTIGLAVVMTAVCFAIASYAIQHTP
                     RGMDPSAGLIGWPVIVSILVFLWITFTLQSMRIRDIGWDPVCVIPAWIALLIADKLVA
                     SKIPAWSLGPEHQGTIVGALINFGLILALTFWPSGEHEGPMPTSGETRRKPPELPRNQ
                     GTASAPAARIARTTGTGFGRRAF"
     gene            complement(1157..3049)
                     /locus_tag="VGA15_05955"
     CDS             complement(1157..3049)
                     /locus_tag="VGA15_05955"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9587273.1"
                     /translation="MAFSVAFAVMASGAGADEAVPQTRLELTAANLASVVDPLMAEWI
                     DKHQGPGAVVVVVTRDGPVFAKGYGFSDIDAKKPFTADATLVRPGSISKLFTGIAVMQ
                     LVDAGRLDLDRDVNAYIDFAIPTPPGGVPVTLRRLLTHRAGFEEHAKGLFSRDREPEP
                     LGRWLAKGLPQRLFPNGDVEAYSNYGLALAGYIVERVSGEPYAFYIQRHILGPLGMSH
                     STFQQPLPDDLEPLMAKGYRGSDKPPLPFFETIAAAPAGALSATGADMGRFIRALMNG
                     GELDGVRILPKARLDQMMAPGNATSAGYLGLVFFGTKIAGHDSLGHDGGTMTFFSELK
                     FFPEQGVGIFVSRDGIGEIKSFTEIPHPATAIAQRFLPKTTEWADARAASFPSEQSVA
                     GIYQTSRRNESSFVRLTALLAQIVVKVDGDGHVRGLSAIWPFGNGAPWKHVQRDLYEG
                     PGARFAFVDDGDSDSYLATPGLRLQRVPWSLDLRWIAPTFVASTAMVFLSLLAWPVAA
                     LWRYRRKRRWSEDRGDRGNFLAIRLVVLVDAAVIGAAAVVFAMSSDFTIFNDALDRWL
                     LVLYAFAWLGVFGAVLTLWAAARFWRHGIGSRWSRVHHALIAASCVMIAWFFLTFHIA
                     GTTLIY"
     gene            complement(3168..3908)
                     /locus_tag="VGA15_05960"
     CDS             complement(3168..3908)
                     /locus_tag="VGA15_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX9587274.1"
                     /translation="MTANSIVRIAGLNKWFGDFHVLRDIDLDVGKGERIVICGPSGSG
                     KSTLIRCINALEEFQEGEIVVDGIELGPNLRRVDEVRREVGMVFQSFNLFPHLTVLEN
                     CTLAPIWVRNIPKKDAEAAAMKYLERVKIPHQASKFPGQMSGGQQQRVAIARALTMNP
                     KVMLFDEPTSALDPEMVKEVLDTMVDLAKEGMTMLVVTHEMGFAREVANRIVFMDAGQ
                     IIEANTPENFFANPQHARTKLFLSQILR"
     gene            complement(3919..5442)
                     /locus_tag="VGA15_05965"
     CDS             complement(3919..5442)
                     /locus_tag="VGA15_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9587275.1"
                     /translation="MSQTAALSFVRRELVPERPAPIKTTGFVGFVRTRLVNSPTNILL
                     TIASILLLWFTVIPALKFLLVDAVWHGTDRNACLAENAGHPVGACWPYIQAKFSQFIY
                     GFYPEPERWRVNLTFTLAALLLLPLLIPPLPAKGLNAGLFFIAFPIVAFFLLHGGGLD
                     GFGVSWTAGLLSGFAESMGDGGRALTNAGAATAVIGLLLTWLGTLVVLLSALISLLIW
                     PLTWLRDQIQTSGRPVWADLAATTAVVSILLFFLAGGFRTGWRALVVSLATFAGIGVV
                     IAAMGLDRGGLPIVDTRLWGGLLVTLVVSVTGIVTSMPVGIALALGRRSTIPLIRIFS
                     ITFIELWRGVPLITVLFFATYMLPLFVPAGFTVDGLVRVLIGIALFSGAYNAEVIRGG
                     LQAIPRGQGEAANALGLSWWKTTALIVMPQALRHVIPGLVNSFIALFKDTSLVSIVAL
                     FDLLGSLRASFSDPVWATPTTAFTGFAFTGMIYFVFCFGMSRYSLFVENRLNAHRRS"
     gene            complement(5439..>6605)
                     /locus_tag="VGA15_05970"
     CDS             complement(5439..>6605)
                     /locus_tag="VGA15_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9587276.1"
                     /translation="ARLRHALGGRAGWRGFFVQILFVAALAWIGYEIAANARANLETQ
                     RITSGFGFLANTAGFDVSQNLIPYSGSDPYTRVLLVGLLNTLVVSIIGIFFATVIGFL
                     VALGRLSPNWLLSRISGGYVELLRNLPVLFQILFWYLAVLAALPNPRQSIALFGSFFL
                     SNRGFVIPKPIGTAGFEPFAVAVLIGIVASLLWRRYARRQLFESGRMIRIWPYVVGSL
                     IGLPLVTSLVFGAPVRFEIPELKGFNFSGGSRLIPEFVALTLALSTYTAAFIAEIVRA
                     GIVSVHKGQMEAGASLGLSRGSTLRLVVVPQALRVILPPLTNQYLNLTKNSSLAVAIG
                     YPDLVSVFAGTTLSQTGQAIEIIGITMGVYLLISLVTSAIMSFYGWRIGRSMGA"
BASE COUNT         1466 a         2058 c         1972 g         1109 t
ORIGIN      
        1 cacccatcat cgagaccgcg aaggtcgacg acgcctttct tattcccgac gaggaagccg
       61 ttctcatcat ttacgagctg ctcgaacacg agggcctgtg cgtaggcggc tcaaccggca
      121 tcaacgtcgc cggcgcgatc cagctcgccc gccagctcgg accgggccat accatcgtga
      181 ccgtgctgtg cgattccgga tatcgctctc agtcaaagct gttcaatccg gatttcatgc
      241 gctcgaaaaa cctccccgtg ccgcaatggc tggagaagcg cagcaagatc gacgtgccgt
      301 tcgagaaggt gtgatcgcgc accagcgcgt ctagagctcc gtttcgatag atcgaaacgg
      361 agctctagat tcttgatttg acgcgttttc ttgacgcgaa ccggtctcca cttcgcttga
      421 aaacgctgct ggaccaatcg ccgtcgccaa cgaccttcaa aatgcgcggc gaccgaatcc
      481 cgttcccgtc gtccgcgcga tgcgtgccgc aggcgccgac gctgtacctt ggttacgcgg
      541 tagctcaggt ggcttccggc gcgtctcgcc agacgtcggc atcgggcctt cgtgttcgcc
      601 gctcggccaa aacgtcagag ctaaaatcag cccgaagttg atcaatgcac cgacgattgt
      661 tccctgatgt tcaggaccca gcgaccatgc ggggatcttg cttgcaacga gcttatcggc
      721 gatgaggagc gcgatccatg ctgggataac gcagaccggg tcccatccga tatccctgat
      781 gcgcatggac tgcagcgtga aggtgatcca caggaatacg aggatggaga cgatcaccgg
      841 ccagccgatc aggccggcgg atggatccat ccccctcggt gtgtgctgga tcgcatagct
      901 cgcaatagcg aaacagactg cggtcatcac cacagccagg ccaatcgtgc tgaggaagta
      961 atgcagccgg ccgagccggg cattgaaacc gaacaggaaa cccaacattg gcgcgtcctt
     1021 ttgggataaa aggccacaaa ttgctttcca gtcggtgaac cggctgcggc ctcgacaggt
     1081 ttatggtttc caatcgcttt cgctgatttc gaaaatcggc gtgctacgga cagtgacaaa
     1141 gccgcatcca acgggtctaa taaatcaacg ttgtccccgc gatgtggaat gtcaggaaga
     1201 accacgcgat catcacgcag ctggcggcaa tcagcgcgtg atggacacgc gaccatcggc
     1261 tgccgatgcc atggcgccag aatctggcgg cggcccacag cgtcagcacc gcgccgaaaa
     1321 cgccgagcca tgcgaaggca tagagcacca gcagccagcg atcgagagcg tcgttgaaaa
     1381 tggtgaagtc tgagctcatc gcaaaaacca ccgcggcagc gccaatgacg gccgcatcga
     1441 ccagcacgac cagccttatg gcgagaaagt taccccggtc gccgcggtcc tcgctccacc
     1501 gcctcttgcg ccggtaacgc catagcgcgg caaccggcca ggccagcagc gacaggaaaa
     1561 ccatcgccgt gctcgcgaca aacgttggcg cgatccaccg tagatcgaga gaccagggaa
     1621 cacgctgcag acgaagaccc ggtgtagcca ggtaagagtc ggagtcgccg tcatcgacaa
     1681 aggcgaaacg cgcgcctggc ccttcgtaga gatcgcgttg aacgtgcttc catggcgcgc
     1741 cattaccgaa cggccagatc gcagataacc ctcgtacgtg cccgtccccg tcgaccttga
     1801 cgacaatctg cgccagcagc gcagttaatc tcacaaaaga cgactcgttg cgcctgctcg
     1861 tctgatagat gccggccaca ctctgctcgc ttgggaagct ggcggcgcgt gcatcggccc
     1921 attccgtcgt cttgggcaga aaacgctggg caatggccgt cgcgggatgg ggtatctccg
     1981 tgaaactctt gatttcgcca attccgtcgc gcgacacaaa aattccgacg ccctgctcgg
     2041 ggaaaaactt caactcgctg aagaatgtca tggttccgcc gtcgtgcccg agcgagtcat
     2101 ggcccgcgat tttggttccg aagaacacga gaccgaggta tccggccgat gtcgcgttgc
     2161 ctggcgccat catctggtcg agccgcgcct tgggcaggat gcgaacgcca tcgagctcgc
     2221 cgccattcat cagcgcgcgg atgaagcggc ccatgtccgc acccgtcgcc gaaagcgcac
     2281 cggccggcgc ggcagcgatg gtctcgaaaa acggcagcgg cggcttgtcc gatccgcggt
     2341 aacctttcgc catcagcggc tcaagatcgt ccggcaacgg ctgctggaat gtcgagtggc
     2401 tcatgccgag cggacccaga atatgccgct gaatatagaa ggcgtagggc tcacccgaaa
     2461 cgcgttcgac gatatagccg gcgagcgcga ggccataatt ggaataggct tcgacatcgc
     2521 cgttgggaaa cagacgttgc ggcagacctt tcgcaagcca acgcccgagc ggttcaggct
     2581 cgcggtccct cgagaacagt cctttcgcat gttcctcgaa accggcccga tgcgtcaaca
     2641 gacggcgcag cgtgacggga acgccgcccg gcggcgtcgg aatggcgaag tcgatataag
     2701 cgttgacgtc gcggtcgaga tcgagccggc ctgcgtccac gagctgcatg acggcgatac
     2761 cggtgaagag cttcgagatc gatcccggcc gcaccagcgt cgcatcggcg gtgaacggct
     2821 ttttggcatc gatgtccgaa aagccatagc ctttggcgaa caccgggcca tcgcgcgtca
     2881 ccaccacgac cacagcacct gggccctggt gcttgtcgat ccactccgcc atcagcggat
     2941 cgaccacgct ggcgaggttg gccgctgtca gctcaagccg cgtctgcggc accgcttcgt
     3001 cggcgcctgc gccagatgcc atcacggcga aagcgaccga gaaagccaag tgcaagaagc
     3061 tcgtgacatg aagggagttc ccggaaaatc cccacgacga gtcgcgagtc acgggtagcg
     3121 aatgaaaatt tcccgattct cttttcgcca ttccctattc gcttcattca ccgcaaaatc
     3181 tggctgagga acagttttgt tcgcgcgtgc tgcgggttgg cgaagaagtt ttccggcgtg
     3241 ttggcctcga tgatctggcc ggcgtccatg aagacgatgc ggttggcgac ttcgcgcgca
     3301 aagcccattt cgtgggtgac gaccagcatg gtcatgccct ccttggccag gtcgaccatg
     3361 gtgtcgagca cttccttgac catttcgggg tcgagcgccg aggtcggctc gtcgaacagc
     3421 atcactttcg ggttcatggt cagcgcgcgc gcgatcgcga cccgctgttg ctggccgccg
     3481 gacatctggc ctggaaactt gctggcctgg tgcggaattt tcacccgctc gaggtatttc
     3541 atggcggccg cctcggcatc cttcttcgga atattgcgca cccagatcgg cgccagcgtg
     3601 cagttctcca gcaccgtcag atgcggaaac agattgaaac tctggaacac catgccgact
     3661 tcgcgccgga cttcgtcgac gcgacgtaaa ttcggaccga gttcgatgcc gtcgacgacg
     3721 atctcacctt cctgaaattc ctccagcgcg ttgatgcagc ggatcagcgt cgatttgccc
     3781 gagcccgaag gaccacagat cacgatgcgc tcgcccttgc cgacatcgag gtcaatgtcg
     3841 cgcagcacat gaaaatcgcc gaaccacttg ttgaggccgg caatgcggac gatcgagttc
     3901 gcggtcatgg tcatttgctc aactacgtcg gtgtgcgttc agccggttct cgacgaacag
     3961 tgaatagcgc gacattccaa aacagaacac gaagtagatc atcccggtga aggcaaaacc
     4021 ggtaaacgcc gtcgtcggcg ttgcccagac cggatcggaa aacgaggccc gtaaactccc
     4081 gagcaaatcg aacagcgcca cgatcgagac cagcgaggtg tccttgaaca gcgcgatgaa
     4141 actgttgacg aggccgggaa tgacgtggcg caacgcctgc ggcatcacga tcagcgcggt
     4201 cgtcttccac caggacaggc ccagcgcgtt cgcggcctcg ccctgcccgc gcgggatagc
     4261 ctgcaggccg cccctgatca cttccgcatt gtaagcgccg gaaaacagtg caatgccgat
     4321 caggacacgt accagtccgt cgaccgtgaa gccagcgggt acgaacaacg gcagcatgta
     4381 ggttgcaaag aacaggacgg tgatcagcgg cacgccgcgc cagagttcga tgaacgtgat
     4441 cgaaaagatc cggatcaatg gaatggtcga ccgccggccc agtgccagcg cgatgccgac
     4501 cggcatcgag gtgacgatac ccgtgaccga caccaccagc gtcaccagca ggccgcccca
     4561 gagccgggtg tcgacgatcg gcaaccctcc ccggtcgagc cccatcgccg cgatcacgac
     4621 gccaatgccg gcaaacgtcg caaggctgac gacgagcgcg cgccatccgg tgcgaaagcc
     4681 gccagccaga aagaacagca ggatcgaaac gaccgccgtg gtggccgcga gatcggccca
     4741 taccggccgg cccgatgtct gaatttgatc gcgaagccag gtcagcggcc agatcagcag
     4801 ggaaatgagg gcactgagca ggacgaccag tgttcccagc catgtcagta gcagtccgat
     4861 gacggccgtt gcggcgcctg catttgtcag cgcgcgcccg ccgtcaccca tgctctcggc
     4921 aaatcccgac aacagcccgg cggtccagct gacgccgaaa ccgtccaggc cgccgccatg
     4981 cagtaggaag aacgcgacaa tcggaaatgc aatgaagaac agtccggcgt taagcccctt
     5041 ggccggcagc ggtggaatca acagcggcaa cagcagcagc gcggcaaggg tgaacgtcag
     5101 attgacccgc catcgctccg gctccggata aaagccgtag atgaactggc tgaatttcgc
     5161 ctggatgtag ggccagcacg cgccgaccgg gtggcccgca ttttcggcaa gacatgcatt
     5221 gcgatccgtg ccgtgccaga ccgcatcgac cagcaggaat ttcagtgcgg gaatgacggt
     5281 gaaccacagc aacaatatgc tcgcaatggt gagcaggatg ttagttggcg aattaaccag
     5341 ccgcgtgcgc acgaaaccga caaaacccgt ggtcttgatc ggcgcgggtc gttcggggac
     5401 gagctcccga cggacgaagg acaaagcggc tgtctggctc atgcgcccat gctccggccg
     5461 atccgccatc cgtagaagct catgatcgcg ctcgtcacca gtgaaatcaa gagatagacg
     5521 cccatggtga tgccgatgat ttcaatcgcc tggccggtct ggctgagtgt cgtgcccgcg
     5581 aataccgaga ccagatcggg atacccgatc gccaccgcca gcgacgagtt cttggtaaga
     5641 ttgagatact ggttggtgag cggcggcagg atgacgcgca acgcctgcgg caccacgacc
     5701 agccgcaatg tcgagccgcg cgacagcccc agcgaagcgc cggcttccat ctgccccttg
     5761 tgaaccgaaa caatgccggc acgcacgatc tcggcgatga aggccgcggt ataggtcgac
     5821 agtgccagcg tcagtgcgac gaattccggg atcaggcgcg agccgccgga aaagttgaac
     5881 cccttcagtt cgggaatttc aaacctgaca ggcgccccga acaccagcga ggtcaccagc
     5941 ggcagaccga tcagcgagcc gaccacatag ggccagatcc tgatcatcct gccactttcg
     6001 aacagctgcc gccgcgcata gcgccgccac agcaatgagg caacgatacc gatcaggacg
     6061 gcgactgcga aaggctcgaa accggcggtg ccgatcggct tcgggatcac aaatccgcgg
     6121 tttgagagaa agaagctgcc gaacagcgcg atgctctgcc gcggattcgg cagcgccgcg
     6181 agcacggcga gataccagaa caggatctga aacaacaccg gcaggttacg cagcagttcg
     6241 acatagccgc cggagatgcg cgacagcagc cagttcggcg acagccggcc caatgcgacg
     6301 agaaatccga tcacagtggc aaagaaaatg ccgatgatcg agaccacgag cgtgttgagc
     6361 aaaccgacca ggagcacgcg ggtataggga tcggatccgg aatatggaat caggttttga
     6421 ctgacgtcaa aacccgccgt gttcgccaga aacccgaagc cggacgtgat ccgctgcgtt
     6481 tccaggttgg cgcgggcgtt ggccgcgatc tcatagccaa tccaggccag cgcggcgacg
     6541 aacaggatct gaacgaaaaa tcccctccag ccggcccggc cgccgagcgc atgccgcagc
     6601 ctggc
//
LOCUS       DASWGM010000196         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2374771,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000196 DASWGM010000000
VERSION     DASWGM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374771"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2157
                     /locus_tag="VGA15_05975"
     CDS             <1..2157
                     /locus_tag="VGA15_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9587277.1"
                     /translation="FTINTSRARGLPGVATILTAEDVRDLGGLPCLFNLEVDPFTGPP
                     YAILAHGVVRHVGDAVAFVVAETVDQARDAIEAIEVEWTPLPAVIGVANAVKKDAPQV
                     WPDHPGNVLFDVPIGDKHATEAAFAKAHAVAEISVVNPRVVTNFMETRAAVAEYDAKR
                     DHLTLTIGSQGSHRLREILCGMVLKMPMEKMRVICPDVGGGFGTKLFPYREYALIAVA
                     ARKLRKTVKWTADRADHFMGDAQGRDNLTTARMALAEDGKFLAMDVDLMGDMGAYLST
                     FGPYIPHGGAGMLPGLYDIQTFHCRVRTVFTNTVPVDAYRGAGRPEAAYVVERLVDAA
                     ARKLGMTPDAIRRKNFIAPRAMPYKTATGKIYDSGDFTAHMKRAMEIANWKEFPKRAK
                     VAKKQGLLRGIGLATYVEVCGTMGEETANVRLDPNGDVSILIGTQSSGQGHQTAYAQL
                     VAEQFGIAPEHVHVYQGDTDQVATGLGTGGSASIPTGGVSVERATRELGKNLREIAAE
                     ALETSSGDLEINEGTVRIAGTDRSVTFADLAKRPGVDPSKLNASATFTSADGTFPNGT
                     HLAEVEIDPATGIIRIVNYVIVDDFGVTLNPLLLAGQVHGGAMQGIGQALMEQAVYDP
                     KDGQLVTGTFMDYALPRAADGPSFVFETHNVPCKTNPLGVKGAGEAGAIGSCPAVVNA
                     IIEGLWREYKIDHIDMPATAERVWIAIREHQRRHSL"
     gene            2282..2728
                     /locus_tag="VGA15_05980"
     CDS             2282..2728
                     /locus_tag="VGA15_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9587278.1"
                     /translation="MLRTVVVVGTLLLGVGAVAAQQDQVNKTQLAMKSNLKSALALLD
                     MVKGKQPYDQAAVNASLAELEDVGKRFSVLFPDSIKGLKPEGDYYASAKVWTDRKGFE
                     AHAASFAKAVGEAKGKVKDLDTLKAAFPAINDECKGCHETYRVKNS"
     gene            complement(2810..3235)
                     /locus_tag="VGA15_05985"
     CDS             complement(2810..3235)
                     /locus_tag="VGA15_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX9587279.1"
                     /translation="MSKTKIMLATLALGATVALAGPAFAELMKVTLDSKSEVPPNTSA
                     GTGTADVDYDAASKKLTWKLTYSGLSGPATAAHFHGPAEAGKNAGVKVPIPNATSSPA
                     EGSATLTDEQAADLTAGKYYINVHTAANPGGEIRGQVTK"
     gene            3403..>3853
                     /locus_tag="VGA15_05990"
     CDS             3403..>3853
                     /locus_tag="VGA15_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9587280.1"
                     /translation="MSRRLFLAAIIVGAAAFGVFWWLTATPTTLAVAEPFHRPNLANG
                     LTAFNAGGCSSCHAVPGQPDRLRLGGGLAIPSPFGTFYAPNISPDPVDGIGRWTEAEF
                     VDAVTKGISPAGFHYFPAFPYPSYQHAKVEDVRDLFAYLKTLAPLSGK"
BASE COUNT          744 a         1182 c         1208 g          719 t
ORIGIN      
        1 ttcaccatca acacgagccg cgcccgcggc ctgccgggcg tggctacgat cctgacggca
       61 gaggacgtca gggatctcgg cggtcttccc tgcctgttca atctggaagt cgatccgttc
      121 accggtccgc cctatgcgat cctggcgcac ggcgtggtgc gccatgtcgg cgacgccgtg
      181 gcctttgtcg tggcggagac cgtcgatcag gcccgcgatg cgatcgaggc gatcgaagtt
      241 gaatggacgc cgctgcccgc ggtgatcggc gtcgccaatg cggtcaagaa ggatgcgccg
      301 caggtctggc ctgaccatcc cggcaacgtg ctgttcgacg tgccgatcgg cgacaagcat
      361 gcgaccgagg cagcgttcgc caaagcgcat gcggtcgccg aaatatcggt cgtcaatccg
      421 cgggtcgtta ccaacttcat ggaaacccgc gcggccgttg ccgaatacga cgccaagcgc
      481 gaccatttga cattgacgat cggcagccag ggcagccatc gcttgcgcga aatcctttgc
      541 gggatggtcc tgaagatgcc gatggaaaag atgcgggtga tctgcccgga tgtcggcggc
      601 gggttcggca ccaagctgtt tccctaccgc gaatacgcat tgatcgcggt cgccgcgcga
      661 aaactgcgca agaccgtcaa gtggaccgcc gaccgtgccg accacttcat gggcgacgcg
      721 caagggcgtg acaatttgac gaccgcacgg atggcgctgg ccgaggacgg caagttcctc
      781 gcgatggatg tcgatctgat gggcgacatg ggtgcctatc tctcgacctt cgggccttac
      841 attcctcacg gcggcgccgg aatgttgccc ggtctctacg atatccagac ctttcactgc
      901 cgtgtccgca cggtgttcac caacaccgtg ccggtcgatg cctatcgcgg agccggacgt
      961 ccggaggcgg cctatgtggt cgagcggctc gtcgacgcgg ctgcgcgcaa gctcggcatg
     1021 acgccggatg cgatccggcg caagaacttc atcgcgccgc gcgcgatgcc ctacaagacg
     1081 gccaccggca aaatctacga ttccggtgac ttcaccgcgc atatgaagcg agcgatggaa
     1141 atagcgaact ggaaggaatt tccgaaacgc gccaaggttg ccaagaagca gggtctgctg
     1201 cgcggaatcg ggcttgcgac ctatgtcgag gtctgcggca cgatgggcga ggagaccgcc
     1261 aacgtccggc tcgatcccaa cggcgacgtt tcgatcctga tcggcacgca gtcgagtggg
     1321 cagggccacc agaccgccta cgcgcaactg gtcgccgagc agttcggcat cgcgcccgag
     1381 cacgttcatg tctatcaagg cgacaccgat caggtcgcca ccggtctcgg caccggcggt
     1441 tcggcctcga ttccaaccgg cggcgtcagt gtcgagcggg cgacgcgcga gcttggcaag
     1501 aatttgcggg agatcgccgc cgaagcgctg gaaacgagca gcggcgacct ggaaatcaac
     1561 gaaggcacgg tgcggatcgc cggcaccgat cgttcggtca cgtttgccga tctcgccaaa
     1621 cgcccgggcg tcgatccgtc gaagctgaac gcgagtgcga ccttcaccag cgccgacggc
     1681 accttcccga acggcacgca ccttgccgag gtcgaaatcg atcccgccac cggcatcatc
     1741 agaatcgtca actacgtcat cgtcgacgat ttcggggtga cgctgaatcc gctgttgctc
     1801 gccggccagg tacatggcgg cgcgatgcag gggatcggcc aggccttgat ggagcaggcg
     1861 gtctacgacc cgaaggacgg ccagctcgtt accggaacgt tcatggatta tgcgctgccg
     1921 cgcgcagccg acggtccgtc attcgtgttc gagacccaca acgtcccctg caagaccaat
     1981 ccgctcggcg tcaagggcgc gggcgaagcc ggtgcgatcg gctcttgtcc tgcggtcgtc
     2041 aacgcgatta tcgaggggct gtggcgcgaa tataaaatcg atcacatcga tatgccagcg
     2101 accgcggaac gggtctggat cgccatccgc gagcatcagc ggaggcatag cctctgatat
     2161 ttgccacatt gccgcggaat gaaacgccgc ctgcggtgtc gggaatgacg attgctgcaa
     2221 cgcgcatcaa gcgcgtcctg cggcgttacc tggaaacccc tgagaaacgg agaactggcc
     2281 gatgcttcga actgtcgttg tggtaggaac attgttgctt ggtgttggcg cggtggcagc
     2341 ccagcaggac caggtcaaca agacccagct tgcgatgaag tccaatctca agagcgctct
     2401 cgctcttctc gatatggtca aaggcaaaca gccttatgac caggccgcgg tcaacgcgtc
     2461 actggccgag cttgaggacg tcggcaagcg tttttcggtg ttgtttccgg acagcatcaa
     2521 aggcctgaag ccggaaggcg actactacgc ttcggcaaaa gtctggaccg acagaaaggg
     2581 cttcgaagcc cacgcggcga gctttgccaa agcggttggc gaggcgaagg gcaaggtcaa
     2641 ggatctcgat accctcaagg cagcgttccc tgccatcaac gacgaatgca agggctgcca
     2701 cgagacctac cgggtcaaga atagctgacg cgggaccgac caaagaaaga ggcggacacg
     2761 tgatgcgtcc gcccctcatc taaaataccg gtcaaacgaa aggtcccgat tacttggtca
     2821 cctggccgcg gatttcgcca cccggattgg ccgcggtgtg gacgttgatg tagtacttgc
     2881 cagcggtcag atcggcggct tgttcgtcgg tcagggtagc gctgccttcg gccgggcttg
     2941 aggtggcgtt cgggatcgga accttgacgc cggcgttttt gccggcttcg gccggcccat
     3001 ggaaatgcgc cgcggtcgcc gggccggata gccccgaata ggtcagcttc caggtcagct
     3061 tcttggaggc ggcatcgtag tcgacgtcgg cggtgcccgt gccggcactg gtattgggcg
     3121 gcacttcgga tttgctgtcg agggttactt tcatcaactc ggcaaacgcg ggtccggcca
     3181 aggcaacggt tgctcccagc gcgagcgtag caagcatgat cttggttttc gacatggttt
     3241 cctcctgttg gcgccaagtg gtggtgccag attcgaaaca acgatttgtc ggttttattc
     3301 ccgtgacggg cttcaccgcg ccaaattttc aaagagccca cggccgcaat agcggccata
     3361 ctcgccatta gaaaatcgaa acggacagag acggatcggt gaatgtcgcg acgacttttt
     3421 cttgctgcca tcatcgttgg tgccgccgcc tttggcgtct tctggtggct aacggcaacc
     3481 ccgaccacgt tggctgttgc cgagccgttc catcggccaa atcttgccaa cggactgacg
     3541 gcattcaacg ccggcggatg ttcctcctgc catgccgtac ccggccagcc cgatcgttta
     3601 aggctcggcg gcgggctcgc catcccgtcg ccgttcggca ccttctacgc gcctaacatc
     3661 tcgcccgatc cggtcgatgg catcgggcgc tggaccgagg ctgaattcgt cgacgcggta
     3721 accaaaggga tttcgccggc cggatttcat tacttcccgg cctttcccta tccgtcgtac
     3781 cagcatgcga aagtcgagga cgtccgcgac ctgttcgcct acctgaaaac gctggcgccg
     3841 ctgtccggca agg
//
LOCUS       DASWGM010000197         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_707239,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000197 DASWGM010000000
VERSION     DASWGM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707239"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /gene="ftsZ"
                     /locus_tag="VGA15_05995"
     CDS             complement(<1..950)
                     /gene="ftsZ"
                     /locus_tag="VGA15_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9587281.1"
                     /translation="MTPEVREPGRSINIFIDDEPPITGARIKVIGVGGGGGNAVNRMI
                     DAGIEGIEFVVANTDLQALKRSRAPVKIQLGGRLTKGLGAGADPEVGRQAALEDTDKI
                     IEVLEGADMVFVTTGLGGGTGTGGAPIVASLATELGALTVAVVTKPFLFEGRRRLQQA
                     EQGLSELHESVDTVITIPNERLFSAVPEGASFADAFKAADDVLRQAVQGISDLITVPG
                     LINLDFADVRTIMQGMGMALMGTGYARGENRAIEATQEAISSPLLEEASIKGARGVLI
                     NITGGHDLTLCEVNNAASIVRQEADEDANIIFGHVIDESMA"
     gene            complement(1084..>2359)
                     /gene="ftsA"
                     /locus_tag="VGA15_06000"
     CDS             complement(1084..>2359)
                     /gene="ftsA"
                     /locus_tag="VGA15_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9587282.1"
                     /translation="KRTAMAKRQNHTVGLDIGTSKVTCVVGEPGEGGLLQIIGIGEAE
                     ARGLRKGVIVNPDAAVEAIKRAIDEAERMSGAQAEEVTINLAGSHIMGFNGQAIVAVA
                     SRDREITGDDVRRAIDSACAIQLPAGREIVDRLPQEFIVDDQDGINDPVGMTGARLAV
                     KVHIVTSPVTARQNAINAVNRSGLIVADMILEQLAAAEATLTEDDKEYGSALVDIGAE
                     TTGLCVYQRGAVQHTAVFPLGGSHFTNDIAFGLRTPIPEAEKIKRTVGCASSASLNDD
                     QRGEMVEVPSVGGRAPRQLSRQILCDILQPRAEEVLMHVSDEIRDSGWNRQLSSGVVL
                     TGGGALLEGMVEIAEQVFDAPVRIGYPERDRFGGLVEDVQSPAWAAAAGLALVSQRAH
                     ALEQRSGAAKRGGSGRLTHFVSKFRNRFSSIL"
BASE COUNT          435 a          771 c          655 g          498 t
ORIGIN      
        1 gccatcgact cgtcgattac gtgcccgaaa ataatgttgg cgtcttcgtc ggcctcctga
       61 cgaacgattg acgccgcgtt gttgacttca cagagcgtca gatcgtgacc gccagtgata
      121 ttaatgagca cgccacgcgc gcccttgatc gacgcttctt ccaggagtgg cgacgagatc
      181 gcttcctgcg tagcttcgat ggcccgattt tcgccgcgcg cgtatccggt ccccatcagc
      241 gccatgccca ttccctgcat gatcgtgcgc acgtcggcaa agtcgaggtt gatcaagccc
      301 ggcacggtga tcaagtcgct gattccctgc actgcctggc gcaaaacatc gtcggccgcc
      361 ttgaaagcgt cggcgaaaga agcgccctcg ggaacggcgc tgaacagccg ctcgtttgga
      421 attgtgatga ccgtgtcaac tgattcatgc agctcgctga gaccctgctc ggcctgctgc
      481 aatcgacgcc ggccttcgaa tagaaacggc ttggtgacga cggccaccgt caaagccccc
      541 aactcggtag ccaatgacgc aacgatcggc gcgcccccgg tccccgtgcc tccgccgaga
      601 ccggtggtca cgaaaaccat gtccgcaccc tcaagcactt cgatgatctt gtcagtgtct
      661 tcgagcgctg cctgccgccc gacttccgga tccgcgccgg cacccaggcc cttggtcaat
      721 ctaccgccca gttgaatctt caccggcgcg cgggaacgct tcagcgcttg caggtcggtg
      781 tttgcgacga caaactcgat gccttcgatg ccggcgtcaa tcatgcgatt gaccgcattg
      841 ccgcccccac cgcccacacc aatgactttt atgcgcgcgc cggtgattgg cggctcgtca
      901 tcgatgaaga tattgatgct tctgccgggt tcgcgaacct caggtgtcat ggtcttttcc
      961 tctcttccaa accgcgaagc tttccacggt ctccttcgtt tattttgggc tggctgaacc
     1021 atgggcatct atatcttgcg gccaacatat tgtgccgccg ggcggccagc atacaacact
     1081 ttgtcaaaga atactactaa aacgattgcg aaatttggac acaaaatgag tcaaacgtcc
     1141 cgacccgccg cgcttcgccg cgcctgagcg ttgctcgagc gcgtgggcgc gttgcgagac
     1201 caacgcgagt ccggccgcag cggcccacgc cggactctgc acatcctcga caagtccgcc
     1261 gaaccgatcg cgttcgggat agccgatgcg aaccggcgcg tcgaagacct gctccgcgat
     1321 ctcgaccatg ccttcgagca gcgcgccgcc acccgtcagc acgacgccgc tcgacaattg
     1381 ccggttccaa ccggagtctc tgatttcgtc tgacacgtgc atcagcactt cttcagcgcg
     1441 cggctgcagg atgtcgcaga gaatctgccg cgacagttgc cgcggtgcgc gaccgccgac
     1501 cgaaggaact tcgaccatct cgccccgctg gtcgtcattg agcgacgcgc tcgaggcaca
     1561 acctacggtg cgcttaattt tctcggcttc cggaatcggc gtgcgcaggc cgaaggcaat
     1621 gtcgttcgtg aagtgcgatc cgcctagcgg aaacacggcc gtgtgttgca cggcgccgcg
     1681 ttgatagacg cataatccgg tggtttccgc gccaatatcg accaaagccg aaccgtactc
     1741 cttgtcgtct tcagtcagcg tcgcttccgc ggccgcaagt tgctcgagga tcatgtcggc
     1801 aacgattaat cctgaccgat ttacggcgtt aatagcgttc tggcgggcgg tcacggggct
     1861 ggtaacgatg tgaactttga ctgccagtcg ggctcccgtc atcccgaccg ggtcgttgat
     1921 tccgtcctgg tcgtcaacga tgaattcctg cggcaggcga tcgacaattt cgcggccggc
     1981 gggaagttga atcgcgcaag ccgaatcgat cgcgcgccgc acatcgtcgc cggtaatctc
     2041 gcggtcgcga ctcgccacgg cgacaatcgc ctgaccgttg aagcccatga tgtgcgagcc
     2101 ggcgaggttg atggtcacct cttcagcctg cgcgccgctc attcgttccg cttcatctat
     2161 cgctcgctta atcgcttcaa cggccgcatc gggattgaca atcacgcctt tgcgcaggcc
     2221 gcgcgcctcg gcttcgccaa tgccgatgat ttgcagcagt ccaccttcgc cgggctcgcc
     2281 aaccacacag gtaactttgc tagtcccgat gtccaggccc acggtgtgat tttgtcgctt
     2341 tgccatagca gttcgtttc
//
LOCUS       DASWGM010000198         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1260407,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000198 DASWGM010000000
VERSION     DASWGM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260407"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VGA15_06005"
     CDS             complement(<1..113)
                     /locus_tag="VGA15_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HEX9587283.1"
                     /translation="MAKTVLIVEDNELNMKLFRDLLEAHGYQTSGTSNGFEA"
     gene            337..636
                     /locus_tag="VGA15_06010"
     CDS             337..636
                     /locus_tag="VGA15_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HEX9587284.1"
                     /translation="MKKPVHNPREVAEIVAIQALSFVAGDPERLGLFLAESGIGPETL
                     RTAAADPRFLGSILDFIMRDDATVKAFASAAQLHPTNIAAARQALGDPDWERDVP"
     gene            633..1919
                     /locus_tag="VGA15_06015"
     CDS             633..1919
                     /locus_tag="VGA15_06015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX9587285.1"
                     /translation="MTSAAPELGGPTCFCRDCLGDLDIAARRCGACGSPRLVRHPALA
                     SLTLAHIDCDAFYATVEKRDNPEIADKPVIIGGGKRGVVSAACYISRTYGVRSAMPMF
                     KALALCPSAVVIRPDMAKYVRVGRDVRHAMQALTPLVEPLSIDEAFLDLAGTQRVHGM
                     IPAKVLARFAQQVERDIGITVSVGLSCNKFLAKIASDMDKPRGFAMLNQDEARAMLAE
                     KPVGFIFGVGPATQERLSQRGFRTIADLQRADEVDLMKQFSTEGRRLWRLARGIDDRR
                     VVPDRGAKTISNETTFETDIRDFATLEKTLWRLSEKVSSRLKSSDLSGLTITLKLKTA
                     DFRQRTRSQSIHAPTQLAAKIFAVSREMLAKEVDGTAFRLMGTGVSALRPGSLADDTD
                     MLDRRSAHAERAIDDLRKKFGNAAVIRGIAYDGPEK"
     gene            2361..3461
                     /locus_tag="VGA15_06020"
     CDS             2361..3461
                     /locus_tag="VGA15_06020"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEX9587286.1"
                     /translation="MRWSYIPRWKFTDSCGRPSVPGALHLGCAHDAHAECHHATRREF
                     LKAGVAIAGAAGFATVVDPREVFAQVRVFPPPPPEVSPIAGLMDTHVHAAPDVFGRSM
                     DDEEVAILYKERGLEALVLKNHVVATADRAWFARKHVAGLKVFGGIVLNSAVGGINPD
                     AVNWMWRMQGGFGRVVWFPTFDADNHVKHFKDAPEAIKVLGADGKVLPAVRDVLKICA
                     EQKLVVHTGHLSPTEALAVIEAGRDAGVSRLVVTHAQFEVVNMSLEQMEKAASMGAKL
                     ELCAMGTLMGPEAHLAWMRHWRRVKVEETAAAVKQLGAQNFVLATDLGQTGNPSPADG
                     LQLFVTDLMKAGVSKDDVTKMGREVTGSLLMG"
     gene            3596..>4170
                     /locus_tag="VGA15_06025"
     CDS             3596..>4170
                     /locus_tag="VGA15_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9587287.1"
                     /translation="MGAAAAAAGLAPAVSSPFVSTALAQAKTLKILQWSHFVPQYDKW
                     FDGFATEWGKKNGVTVTVDHMPHLELPARAAAEVSAGAGHDIFAFNGSGGPHLYEKHL
                     VDLSSLVSEVEEKHGKVQQIGRQIAYNEGTKVWSALPAYYIRFPGLYRKDLWDEIGMK
                     PDTWEDIRVGGAKLKAKGSPIGIGLGHSVDP"
BASE COUNT          791 a         1298 c         1332 g          749 t
ORIGIN      
        1 gcctcgaaac cgttgctggt gccggaggtc tgatagccat gcgcttccaa cagatcgcga
       61 aagagcttca tattgagctc gttgtcctcc acgatcagga cggtttttgc catccgtccc
      121 tccaatcccc aggcgacagg ccggcatgcg gtgcatcagg cgacgccgcg ggcgtgtcga
      181 aaagtgaatt caaactagcg tcagattcgc tctaagcagt taagtccggg ccatctttaa
      241 gcgaagtggt ttccacttgc ttgaacacgt tccgcagact cgggcatgat ggtttcaaaa
      301 tagagaccat aagttacgga aaggcaaaca catcagttga aaaaaccggt ccataatccc
      361 cgggaagtgg ctgaaatcgt tgcgattcag gcgctttcct ttgtcgccgg cgatcccgaa
      421 cggctgggcc tgttcctggc cgaaagcgga atcggccccg agacgttgcg cacagccgcc
      481 gccgatccgc ggtttctcgg ctcgatactc gatttcatca tgcgggatga tgcaaccgtg
      541 aaggcgttcg cgagcgccgc gcagctgcat cccaccaaca ttgccgcagc ccgccaggcc
      601 ctgggcgacc ccgattggga gcgcgatgtg ccgtgacgtc agccgcgcct gagcttggcg
      661 gcccaacctg cttttgccgg gattgcctcg gcgatctcga catcgctgcc aggcgatgcg
      721 gcgcatgcgg ctcgccacgg ctggttcgtc accctgcgct cgcttcgctc accctcgccc
      781 acatcgattg cgatgcgttc tacgcgacgg tggagaaacg cgacaaccct gaaatcgccg
      841 acaagcctgt gatcatcggc ggcggcaagc gcggcgtggt ctccgcggcc tgctacatct
      901 cccggaccta tggcgtgcgc tcggcgatgc cgatgttcaa ggcgctggcg ttgtgcccgt
      961 ccgcggtcgt gatccggccg gacatggcga agtatgtcag ggtcggccgc gacgtgcgcc
     1021 acgccatgca ggcgctgacg ccgctggtcg agccgctctc gatcgacgag gcgtttctgg
     1081 atcttgccgg cacccagcgc gttcacggca tgatccccgc aaaagtgctg gcgcgcttcg
     1141 cccaacaggt cgaacgcgac atcggcatca cggtatccgt cggtctctcc tgcaacaaat
     1201 tcctggccaa gatcgcatcc gacatggaca agccgcgagg ctttgcaatg cttaaccagg
     1261 acgaagcgcg cgccatgctg gccgaaaaac cggtcggatt cattttcggc gtgggacctg
     1321 cgacccagga gcggctgtcg cagcgcggct ttcgcaccat cgccgatctg cagcgcgccg
     1381 atgaagtcga cctgatgaag caattctcga ccgagggccg ccggttgtgg cggctggcgc
     1441 gcggcatcga cgatcgtcgc gtggtgcccg atcgcggcgc caagaccatt tccaacgaga
     1501 ccacgttcga gaccgatatt cgcgatttcg cgacgctgga aaaaacgctg tggcggttgt
     1561 cggaaaaggt ctcttcacgc ctcaagagca gcgatctctc cggcttgacc atcacgctga
     1621 aattgaagac ggccgatttc aggcagcgca cccgctcgca atcgattcat gcaccgaccc
     1681 agctggcggc gaaaattttc gcggtttccc gcgagatgct ggcgaaggaa gtcgacggca
     1741 ccgcgttccg gctgatgggc accggcgtca gcgcgttgcg cccgggatcg ctggccgacg
     1801 acaccgacat gctcgaccgc cgctcggccc atgccgaacg cgcgatcgat gaccttcgca
     1861 agaagtttgg taacgccgcc gtgatccggg gcatcgccta tgacgggccg gagaagtgaa
     1921 ccacatccaa ttaagggcgc gtacccataa atcgagcttg attgctactc gatgtccgat
     1981 atgcggccga tagcggacca aatccgcatc gcagcgaaac gacgctaatt gcagacgtac
     2041 aacctcgctg acgacgctgc gggcgaaagc aagcgcatcg tccttggctc acgatggctt
     2101 ggaggaacgc tgcacgctga agcgggacgt tcctgcacgg tacggcggcg gcttataggc
     2161 gtccgggggc gaattcacga ccgcctcggt ggcgatgtcc ccccacattg gatcatttta
     2221 aatgagcagg tgccgacgga ttcgtgctat agtgacctgg caactcagga caaaaaaggg
     2281 gaatggcgag gcacgcacag gttcctgtgc cggtgctggc cgacattcgc cgcgcccctc
     2341 acaaccgagg aggcggttcg atgaggtggt cctatatccc gcgatggaag ttcaccgata
     2401 gctgcggtcg gcccagcgtg cctggcgcgc tccacctcgg ctgcgcgcac gacgcccacg
     2461 cagagtgtca tcatgcgacg cgccgcgagt tcctcaaggc tggggtcgcg atcgccggcg
     2521 cagccggttt cgcgacagtg gttgacccgc gcgaggtgtt cgcgcaggtg cgcgtcttcc
     2581 cgccgccgcc gccggaagtc agcccgatcg ccggcctcat ggacacgcac gtccacgccg
     2641 cgcccgacgt cttcggccgg tccatggacg acgaggaggt ggcgatcctc tacaaggagc
     2701 gcgggctcga ggcgctcgtc ctcaagaacc atgtggtggc caccgcggac cgcgcctggt
     2761 tcgcgcgcaa gcacgtcgcc ggcctcaagg tgttcggcgg catcgttctc aactccgcgg
     2821 tgggcggcat caaccccgat gccgttaact ggatgtggcg gatgcaaggc ggctttggcc
     2881 gcgtcgtgtg gttccctacc ttcgatgccg acaaccacgt caagcacttt aaggatgcgc
     2941 cggaggccat caaggtgctc ggcgccgacg gcaaggttct ccccgcggta cgcgatgtgc
     3001 tgaagatctg cgccgagcag aagctcgtcg tccacaccgg gcacctgtcg ccgaccgagg
     3061 cgctcgccgt catcgaggcc ggtcgcgatg cgggcgtcag ccgcttggtc gtcacccacg
     3121 cccagttcga ggtcgtcaac atgagcctcg agcaaatgga gaaggccgcc tcgatgggcg
     3181 cgaagttgga actgtgcgcg atgggtacgc tgatggggcc ggaggcgcat ctcgcctgga
     3241 tgcggcactg gcggcgggtg aaggtcgagg agaccgcggc tgcggtgaag cagctcggcg
     3301 cgcagaactt cgtcctagcg accgatctcg gccagaccgg caacccgagc ccggcggacg
     3361 ggcttcagct gttcgtcacc gacctcatga aggccggggt cagcaaggac gacgtgacca
     3421 agatgggtcg cgaggtaacc ggcagtctgc tgatgggttg agccgccgtc accggcctcg
     3481 cgccttcgtt gcaatgtggt tagtcaagac aacgacttgt caggaggagg caaacatgcg
     3541 gtcagaacga gaaatttcca cgtcagccgt cacacgacgc gggttcttga agcgggtggg
     3601 cgccgcggct gcagctgcgg gtctggcgcc ggcggtttcg tcgccgtttg tgtctaccgc
     3661 tcttgcacag gccaagacgc taaagatcct ccagtggagc catttcgtgc cgcagtacga
     3721 taaatggttc gatggtttcg caacggagtg gggcaagaag aatggcgtca ccgtaaccgt
     3781 tgatcacatg ccgcatctgg agctaccggc gcgcgccgcg gccgaggttt ctgcgggagc
     3841 cggacatgac attttcgcct tcaatggatc gggcggcccg cacctgtacg aaaaacatct
     3901 cgtcgatctc agcagtctcg tcagcgaggt ggaggagaag cacggcaagg tccagcagat
     3961 cggtcgccag atcgcgtaca acgagggtac gaaggtctgg agcgcgcttc cggcctacta
     4021 catccggttc cccggccttt accggaagga cctgtgggac gagatcggga tgaaacccga
     4081 tacctgggaa gatatccgcg tcggtggggc caagctgaag gcgaagggca gcccgattgg
     4141 tatcgggctt gggcactccg tcgacccgaa
//
LOCUS       DASWGM010000199         4909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1398990,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000199 DASWGM010000000
VERSION     DASWGM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398990"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VGA15_06030"
     CDS             <1..1414
                     /locus_tag="VGA15_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587288.1"
                     /translation="SFHALLGENGAGKSTLVKCIMGYYHPTEGDVLVGGREQAIANPK
                     DAHALGLGMVYQHFTLVPAMTVAENLVLARDDVPAVVDWPKEKKQLEAFLARMPFQVP
                     LNARVSDISAGERQKCEILKQLYLKRRFLILDEPTSVLTPGEADEVLGMLREMVVNGG
                     LTILMITHKFREVMAFADDVTILRRGKLAGYGRVADLTPDSMARMMIGAEELTIQPAR
                     VGEVGEARLELSRLNALDDAGAVAVHDVSLAVRGGEIVGIAGVSGNGQRQLVEVLAGQ
                     REAESGEIRVAGDIYHASREEMRRHKMSLLPEEPLKNACVGGMSVADNIAFREFDRAP
                     FASGGWWLNRAAFRDDADKKIAQYKIKTRTPDTPISALSGGNVQRAVLARELGGDVEV
                     LIAANPCFGLDFAAVAQIHAEIMAARNRGAAVLLVSEDLDELLELSDRLVVMFHGQFV
                     YEARASEADLTEIGRHMAGH"
     gene            1475..1975
                     /locus_tag="VGA15_06035"
     CDS             1475..1975
                     /locus_tag="VGA15_06035"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX9587289.1"
                     /translation="MDILNMTTSELDEYFLRRSFDVARRALVNGNHPFGAILVDRNRN
                     VLIEAENGYMPSRDGTAHAERLLATQACTTFGPDVLGGATLYSSAEPCAMCAGAIYWA
                     GIGRLVYGLSEHRLRAVTGNHPENPTLDLPCREVFKSGQRATEVLGPMLEDEAEALHA
                     GVWSKE"
     gene            complement(2065..3168)
                     /locus_tag="VGA15_06040"
     CDS             complement(2065..3168)
                     /locus_tag="VGA15_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587290.1"
                     /translation="MSYRIKAIAAAMLSLATLAPAGLASAADLPYKAKAKPIADLPFF
                     FVIDDRVTYSWMPKGTDPGAFSVRPDGTINGKTAKSVYSFTHFDAWAYGTNFFTISLF
                     KSDHNDPASPCTNAGTIFGVPANCAGATEIYGLFRSTFGWNEIFNTKAFTMGPLHNVS
                     FEVGMDANSENNFLAPAKRDFVAGLQFAFDLPYKGYFNVAPLAYKEINHNAFEQCGLF
                     PGGVPGVTCLADGNTDFKTTWAVETNYYMDLGFLPENMQFFSISGRAAWYGPKGDQNS
                     PLAGLGVGRFSTATKTEFNGEPIRLTFDASKAFMGPKYSHFVDVWVAYRYWQNKFGLD
                     HNASPGVCTIAATGQSTNSCTESTVYTGVTVKF"
     gene            3764..>4909
                     /locus_tag="VGA15_06045"
     CDS             3764..>4909
                     /locus_tag="VGA15_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9587291.1"
                     /translation="MLDPTPTGQRLGETPLLQTVGLTKRYGNFLANDQIDIDIWPRQI
                     HALLGENGAGKSTLVKAIYGLIQPSDGEMRWQGEKMTLSGPSEARARGIGMVFQHFSL
                     FDNLTVAENVALGLDGKESFKDMSARLEEVSNVYGLPLDPRREVWQLSVGERQRIEIV
                     RALMQNPKFLILDEPTAVLTPQEADQLFVVLDRLKAEGRAVLYISHKLEEVKRLCDTA
                     TILRGGKKISTCNPQEETAASLARMMVGADIKEVKAAAGRRTTVPRLVVNDLSLEPDD
                     PHGVRLDHISLELKGGEILGIAGVAGNGQDELFAALSGERLAQDPSTVVIDGIAAGHL
                     SITQRRKLGAAFVPEERLGHGTAPRMKLSENALLTGHAASGMVRHGFV"
BASE COUNT          967 a         1463 c         1565 g          914 t
ORIGIN      
        1 ctcgtttcat gcgctgctcg gcgaaaacgg cgccggcaag agcacgctgg ttaaatgcat
       61 catgggctac tatcacccga ccgagggcga tgtgctggtc ggcggccgcg aacaggcgat
      121 cgccaatccc aaggacgccc atgcactcgg cctcggcatg gtctaccagc attttacctt
      181 agtgccggcg atgaccgtgg ccgagaacct ggtgctggcg cgcgacgatg tcccggccgt
      241 tgtcgactgg cccaaggaaa aaaagcaact ggaggcgttt ctggcacgga tgccgttcca
      301 ggtgccgctc aacgccaggg tctccgatat ctccgccggc gagcggcaaa aatgcgaaat
      361 cctcaagcag ctttatctca agcgacgctt tctgattctt gacgaaccga cctcggtgct
      421 gacgccgggc gaggctgacg aagtgctggg catgctgcgc gaaatggtcg tcaacggcgg
      481 cctgactatc ctgatgatca cgcacaaatt ccgtgaggtg atggcgttcg ccgacgacgt
      541 gacgatcctt cgccgcggca agctcgccgg ttacggccgc gtcgcggatc tgacgccgga
      601 cagcatggcc cgcatgatga tcggcgcgga agaattgacc attcagccgg cccgggtcgg
      661 agaagtcggc gaggcacggc tcgaattgag ccgacttaac gcgctcgacg atgccggtgc
      721 ggtcgcggtc catgacgttt cgcttgccgt ccgtggcgga gagatcgtcg gcatcgccgg
      781 cgtttccggc aacggccagc gccagcttgt cgaggtgctg gccggtcagc gcgaggccga
      841 aagcggcgaa attcgcgtgg ccggcgatat ctaccacgcc agccgcgagg aaatgcggcg
      901 gcacaagatg tcgctgctgc cggaagagcc gctaaagaac gcctgcgtcg gcggcatgag
      961 cgtcgccgac aatatcgctt tccgcgagtt cgaccgcgcg ccgtttgcca gcggcggctg
     1021 gtggctcaac cgcgcggcgt ttcgcgatga cgccgacaag aagatcgcgc aatacaagat
     1081 caagacccgc acgcccgata cgccgatctc ggcgctatca ggcggcaatg tgcagcgtgc
     1141 ggtgctggcg cgtgaactgg ggggcgatgt cgaggtcctg atcgccgcca atccctgctt
     1201 cggcctggat ttcgccgcgg tggcgcagat ccatgccgag atcatggccg cgcgcaaccg
     1261 cggcgccgcg gtactgctgg tgagcgagga cctcgacgag ctgcttgaac tgtcggatcg
     1321 cctggtcgtg atgtttcacg gccagttcgt ctatgaagct cgcgccagcg aagccgacct
     1381 caccgagatc ggacggcaca tggcggggca ctaatccttc cggttgcctg tcgtacctgc
     1441 cgtctgcatg atggagcggg cagcaggagc ggccatggac atcttgaaca tgactacgtc
     1501 ggagctcgat gagtattttt tgcgccggtc gttcgacgtc gcccgccgcg ccctggtaaa
     1561 tggcaatcat cccttcggcg ctatcctcgt cgatcggaat cgcaacgtgc tgatcgaggc
     1621 cgagaacggc tacatgccct cccgcgacgg caccgcccac gccgaacggc tgctcgcgac
     1681 gcaggcctgc acgacattcg gcccggatgt cctcggcggt gccacgcttt attcgtccgc
     1741 cgaaccctgc gcgatgtgcg ccggcgccat ctactgggcc ggcatcggcc gcctggtcta
     1801 cgggctgagc gaacatcgat tgcgtgccgt caccggaaat catcccgaga atccgacgct
     1861 cgatctgccc tgccgcgagg tgttcaagag cggacaacgc gcgaccgagg tcctcggacc
     1921 gatgcttgag gacgaggcgg aagcgctgca cgccggcgtc tggagcaaag aataaccctc
     1981 gagattttca agtcgccgaa atagagatgg cgccgcgggg tccatcgcgg cgccatcgtg
     2041 ttcacctgga ggaggatcag gtggttagaa ctttaccgta acgccggtgt agacggtcga
     2101 ttcggtgcag gaattggtgc tttgaccggt cgcggcgatt gtgcagacgc cgggggaggc
     2161 gttgtggtcg aggccgaact tgttctgcca gtagcggtag gcaacccaga catcaacgaa
     2221 gtgagagtac ttcggtccca tgaaggcctt gctggcgtcg aacgtcaggc ggatcggttc
     2281 gccgttgaac tccgttttgg tcgcggtgct gaacctgccg acaccgagcc cggccagcgg
     2341 cgagttctgg tcacctttcg gtccgtacca ggccgcacgg ccgctgatgg agaagaactg
     2401 catgttttca ggcaggaagc ccagatccat gtagtagttg gtctcaaccg cccaggtggt
     2461 cttgaaatcc gtgttgccgt cggcaaggca ggttaccccg ggaacccctc ccggaaacag
     2521 accgcactgc tcgaacgcgt tgtggttgat ttccttgtag gccaagggcg ctacgttgaa
     2581 gtagcccttg tagggaaggt caaaggcgaa ttgcagaccg gcgacgaagt cgcgcttggc
     2641 cggggccagg aagttatttt ccgaattggc gtccatgccg acttcgaacg aaacgttatg
     2701 caaaggcccc atggtaaagg ccttggtgtt gaagatttcg ttccagccga acgtgctgcg
     2761 gaacaggccg taaatttcag tcgcgccggc gcagttcgcc gggacgccga agatggtgcc
     2821 tgcgttcgtg caaggcgaag cgggatcatt gtggtccgac ttgaacaacg aaatggtgaa
     2881 gaagttggtg ccgtaggccc aggcgtcgaa gtgggtgaac gaatagacac tcttcgcggt
     2941 cttgccgttg atcgtgccat ccggccggac gctaaaagca ccgggatcgg tacctttggg
     3001 catccatgaa taggtcaccc ggtcgtcgat gacgaagaag aacggcaggt cggcgatcgg
     3061 ctttgccttg gccttgtacg gcaaatcggc agcgctggcc aagccagccg gtgcgagtgt
     3121 ggcaagtgac agcatcgctg ccgcaattgc tttaatccgg taggacattt ctgagttccc
     3181 caaatttgac gatcgaaaga tcaaggcccg ctgcggccac acttgcgccg ttcgacggac
     3241 gcgagaagcc tcaagtttcc ccatggcttg gcgcaagttt catcaggaat ggcccgtgtg
     3301 gggcgagttg catgcaactc aacgcccacc accgggttga ccgtgccaac tgaaaggcaa
     3361 actcggggca cggttgtgcc cgtttctatt ggctttttga gacaatttac cgcaacgctg
     3421 gcggccgggc cggcccgcaa tttgatactt gccgggcgcc cccccgatcg gatgatcctg
     3481 ctcacgttca cggcatggct gacccgacgg ttccgatttg ctctcgaccg tcacaattct
     3541 gcaacagact tttcccctct tcgcgtgaaa cgcgacatcg acccgccacc gacgcgaatc
     3601 acgccggcaa aggcggaggt aatggaggaa aggcggagag ggaaattgca ggccgccgcc
     3661 tgccttcgag ttgggcaaac gctggcgctt tttcactact tacgccggcg tccaaactag
     3721 cccagtattg aggcagcaat cccttgagcg cgcgtgacaa cgaatgttag atccgacgcc
     3781 aaccgggcag cgtctcggcg aaacgccgtt actacagacg gttgggctga ccaaacgcta
     3841 cggcaatttt ctcgccaacg accagatcga tatcgatatc tggccgcggc agatccatgc
     3901 gctgctcggc gagaacggtg cggggaaatc gacgctcgtc aaggcgatct acggattgat
     3961 ccagccgagc gatggtgaaa tgcgctggca gggcgagaaa atgaccctct ccgggccatc
     4021 ggaagcccgc gcgcgcggca tcggcatggt gttccagcat ttctcgctgt tcgacaatct
     4081 caccgtcgcg gagaacgtgg cgctgggtct ggacggcaag gaatcgttca aggacatgtc
     4141 ggcgcgtctg gaggaagtgt ccaatgttta cggacttccg ctcgatccca ggcgcgaggt
     4201 ctggcaactg tcggtaggcg aacgtcagcg catcgaaatc gttcgggcgc tgatgcaaaa
     4261 tcccaaattc ctgatcctgg atgagccgac cgcggttctg accccgcagg aagccgacca
     4321 gttgttcgtc gtgctggacc gactgaaagc cgagggccgc gccgtcctct acatcagcca
     4381 caaactggaa gaggtgaaac ggctgtgcga tacggccacg atcctgcgcg gcggcaagaa
     4441 gatctcgacc tgcaacccgc aagaggaaac cgccgcatcg cttgcgcgca tgatggtcgg
     4501 ggccgacatc aaggaagtga aggccgccgc cggacggcgg accacggtgc cgcgcctcgt
     4561 tgtcaacgat ctcagcctgg agcccgacga tcctcacggc gtcaggctcg atcacatctc
     4621 gcttgaattg aagggtggcg aaatcctggg catcgctggc gtcgccggca acggccagga
     4681 tgagcttttc gcagcgcttt cgggagagcg gctggcgcaa gaccccagca cggtggtgat
     4741 cgacgggatt gcggcaggac atctttcgat cacgcagcgc cgcaagcttg gcgcagcttt
     4801 cgtgcccgaa gaacgattgg gtcacggcac cgcgccgcgc atgaaacttt ccgagaatgc
     4861 cctgctgacg ggtcacgcgg ccagcggaat ggtccggcac ggtttcgtc
//
LOCUS       DASWGM010000200         6579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2511392,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000200 DASWGM010000000
VERSION     DASWGM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2511392"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VGA15_06050"
     CDS             complement(<1..1002)
                     /locus_tag="VGA15_06050"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9587292.1"
                     /translation="MSGVASTLQGQGTDGTHALVSGLTPELLRRRVGQRRQILAVQVA
                     SYSLGALVLLVYAYSGTVPIIIPFSYFLCGLTLTGFFIVLSETHFNDRFEDHYLTIFQ
                     ISGNVALQLGYLLWAPEIGYAFLTVLFLIFGFGALRMTSRQAIIAWTLTTLGLAPIFL
                     LTDLRIEMPVALYTERVAAMLGFVITIGQCAFVGLYGSSMRKILYKRGVELKAANKRI
                     EELAELDELTGSFNRRCIMRMLGDEITRAHRRKTPCAIALIDLDWFKRVNDHYGHPTG
                     DEVLRTFAITVFANIRHIDRFGRYGGEEFLLVLPDTPDDEAVRTLNRLRAIVADLDWS
                     "
     gene            1436..2005
                     /locus_tag="VGA15_06055"
     CDS             1436..2005
                     /locus_tag="VGA15_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9587293.1"
                     /translation="MSATQAATDDVLIARIAQGDRLAMQVLYGRHHVRVFRFGFRLVR
                     DEQVAEDLISEVFLDVWRQAGKFEGRSAVSTWLLAITRFKALSALRRRKDAELDDEAA
                     NAIEDASDDPEVAVQKKDKSGALRKCLTALSPEHREIVDLVYYHEKSVEEVAEIVGIP
                     ENTVKTRLFYARRKLADLLKAAGVERGWP"
     gene            2002..2700
                     /locus_tag="VGA15_06060"
     CDS             2002..2700
                     /locus_tag="VGA15_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587294.1"
                     /translation="MMATSKKILEQEPGEIEMLLPWHAAGTLDARDARRVDEALARDP
                     ELAKQYAVIREEYAETIHLNESLGAPSVRAMQKLFAAIDGEPARKPSVSFNFSARIAG
                     FFARLSPRALAWSASLGALALLLQAGVIGAMLVKNQNASFQTASLSTNEPITRDLGRI
                     TPPRALVRFAPDARVADITALLDNYQASIIDGAKGGLFRLQFGDRAMGRAEFAGLIRR
                     LQNEKIVSLAVDAQ"
     gene            2738..4465
                     /locus_tag="VGA15_06065"
     CDS             2738..4465
                     /locus_tag="VGA15_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9587295.1"
                     /translation="MVRDPANWLTKMRPAVLALSVAALPLGYLDGATAHAQSIMRSPN
                     LNIGARIPSINPTVAPRINPDIAGRVVSGADGRPPRVNITSVTRSTPRIGVTSTLPYA
                     RYSPNLYPACSYAYRDSDGECRDQPVTSADGGGAGGASGKSKNNGARRNTTLTALNLR
                     TIANELVAEIDGSLSDAQADELARRHRLERIGSQNFPLIGGTIGLFRVVDRRTVETAS
                     RELATDASVRSVQPNFRYVLQDQKVALTEGDPAQYALQKLRLPQAHALAHGANVTIAV
                     IDSGIDVRHPELANAVVDSFDALGSREGPHVHGTGIAGAIVSHARLMGSAPAARILAI
                     RAFGVAASGAASTSFVILKGLDYAAAHGAQIVNMSFAGPKDGLIERGIAAAAAKGILM
                     VAAAGNGGPKSPPLYPAANPNVMAVSATDAQDRLFAASNRGSHIAIAAPGVDIFLPAP
                     NEKYQMTSGTSFSAAYISGLAALMLERNPVLKPDQVRAILVKTARDLGSPGRDDLFGA
                     GEADAFAAVSAVVTAPAVPVAAVPDGHAAEKISGHQEVPVTRDLNQPGTAMASDKAAV
                     REANRPAAQ"
     gene            4944..5465
                     /locus_tag="VGA15_06070"
     CDS             4944..5465
                     /locus_tag="VGA15_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03809 family protein"
                     /protein_id="HEX9587296.1"
                     /translation="MTHRTDVARGRDVVARWCDLAERRLEYLTELFETGRWRRYYSER
                     AFLDNIQEAKTAVEIWRELSTREASSDNFAVDISWLGRTRATRAMLPSGETSREWVHR
                     FQPQPPQIPAEPVPRDVSVAAEPGHVCSDAAPSAPEPPAPALDRSLDPVLDRLTRDIA
                     AITERYPLLRNVM"
     gene            complement(5515..>6579)
                     /locus_tag="VGA15_06075"
     CDS             complement(5515..>6579)
                     /locus_tag="VGA15_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03808 family TAT-translocated repetitive
                     protein"
                     /protein_id="HEX9587297.1"
                     /translation="AGGASMFQGEGTNSVGLSNLTLDGAGIALPVRRGLVHCLGGRDI
                     RVTDCEIAGSGGNGIWFENVSGDISGNIITKSATTAIVSLDAQGLLVSRNPIQGTNDN
                     GIEILRSTVGDDGTQVLDNRIADIKAGPGGSGQYGNAINAFRAGNVIVRGNRISNCDY
                     SAVRGNSASNIQISGNSVSDVREVALYSEFSFEGAVIANNTVDGAALGVSVCNFNDGG
                     RIAVVQGNIIRNLLPKRPIGTAPDDDAGIGIYVEADTAVTGNVIENAPAFGIIAGWGK
                     YLRDVVISGNVIRNAFVGVGVSVVPGAGTALINNNMISETPRGAVVGLDHARAITPDL
                     SAEGAQRFAQVVIGTNAVRR"
BASE COUNT         1265 a         2023 c         2055 g         1236 t
ORIGIN      
        1 gctccagtcg agatcggcga cgatcgcgcg cagccggttg agcgtgcgca cggcttcgtc
       61 atcgggcgta tcgggaagca ccagcaggaa ttcctcgcca ccgtagcggc caaacctgtc
      121 gatgtggcgg atgtttgcga atacggtaat ggcgaatgtc ctgaggactt cgtcgccggt
      181 ggggtggcca taatgatcgt tgacgcgctt gaaccagtcg agatcgatca gtgcgatcgc
      241 gcagggcgtc ttgcgacgat gggcgcgggt gatctcgtca cccagcatcc gcatgatgca
      301 gcggcggttg aacgagccgg tcagctcgtc gagttcggcg agctcttcga tccgtttgtt
      361 ggctgccttg agttcgacgc cgcgcttgta gagtatcttg cgcatcgaac tgccgtacag
      421 tcccacgaac gcacactgtc cgatcgtgat gacgaagccg agcatcgcgg caacgcgttc
      481 ggtatagagt gcgaccggca tttcgattcg aaggtcggtc agcagaaata tcggcgccag
      541 gcccaatgtg gtgagggtcc aggcaatgat ggcctgccgc gacgtcatcc gcaacgcgcc
      601 aaatccgaaa atcaggaaca gtacggtcag gaacgcatat ccgatctcgg gcgcccacag
      661 caggtatcca agctgcagtg cgacattgcc ggagatctga aagatcgtga gatagtggtc
      721 ctcgaacctg tcgttgaaat gggtctccga cagcacaata aaaaatcccg tcagcgtcag
      781 gccgcacagg aaatagctga atggaatgat gatcgggacc gtaccggagt aggcatagac
      841 cagcaggacg agtgcgccga gcgaatagct tgcgacctgc accgcgagga tctgacgacg
      901 ctggccgaca cggcgtcgca gcagctccgg ggtcaacccg ctgacgagag cgtgcgttcc
      961 gtcagtgccc tgaccttgaa gcgttgaagc tacgccgctc atgtcccgcc gtcggtgaat
     1021 tgcatttagt tgaagtttag ggagtaaagc ctttaggttt ggtatctttc acagccgaat
     1081 ctcgctcgcc caaggtcgcc gttgcccttg tttcgcaatg gaaattagcc gcaatgcgta
     1141 ccgtgacgct actgggcgca gggtggcgtc gccggcatag gcaggcgggg tcaaacaggt
     1201 gttttgcgca ccaagccttg ctaaggtagc ggacgaccgt ggcgcgaaga aagtattgcg
     1261 gtattatggt actaattttg gggttttgtt cgttcccgcg ctctcggtgg cggccgagtc
     1321 aacggcgtct gtgtgctaga tcacagaagg gccactatga cgataatttg aagaattttg
     1381 ccttttggat cgaaccaccg ggcagcccaa tccgaccaac cttgcgagac ggccattgag
     1441 tgcgacacag gcggctacgg acgacgtcct gatcgctcgg atcgctcaag gcgaccggct
     1501 tgccatgcag gtactgtatg gcaggcacca tgtaagagtg tttcgcttcg ggtttcgcct
     1561 cgtgcgggat gaacaggttg cggaagacct catcagcgag gtttttctcg atgtgtggcg
     1621 ccaggctggc aagttcgaag gccgatccgc cgtttctacc tggctgctgg cgatcacgcg
     1681 attcaaggcc ctgtctgcgc tgcgccgcag gaaggacgct gaattggacg atgaggccgc
     1741 caatgcgatc gaggatgcgt ccgacgatcc ggaagtggcg gtgcagaaga aggacaagag
     1801 tggagcgttg cgcaagtgcc tgaccgccct ttcgccggag catcgggaga tcgtcgatct
     1861 cgtctactac cacgagaaat ccgtggaaga agtcgccgaa atcgtcggta taccggagaa
     1921 caccgtgaag acgcgcttgt tttatgcgcg cagaaaattg gccgatctgc tgaaggcagc
     1981 cggcgtggag cgaggctggc catgatggca acgagcaaga aaatactgga gcaagagcct
     2041 ggtgaaatag aaatgctgtt gccatggcat gcggcaggca ccctcgatgc gcgcgatgcc
     2101 cgccgcgtcg acgaagcgct ggcccgggat cccgagctcg cgaagcaata tgccgtgatc
     2161 cgggaagaat acgccgaaac catccacctc aacgagagcc tcggggcgcc gtcggtgcgc
     2221 gccatgcaga agctgttcgc ggcgatcgac ggagagccgg cgcgcaagcc gtcggtgtcg
     2281 ttcaatttct cggcgcggat cgccgggttt ttcgccaggc tctcaccgcg ggcgttggcc
     2341 tggtcggcaa gcctgggggc gctggcgttg ttattgcagg ccggcgtgat cggcgcgatg
     2401 ctggtcaaga accagaacgc ctcgttccag accgcatcgc taagcaccaa cgagcctatc
     2461 acccgcgatt tagggcggat aacgccgccg cgcgcgctgg tgcggttcgc gccggacgca
     2521 cgcgtcgccg acatcacggc actgctcgac aactatcagg cctcgatcat cgacggcgcc
     2581 aaaggcggcc tgtttcggct gcaattcggt gatcgagcga tgggcagggc cgaatttgcc
     2641 ggcttgatcc gcaggctgca gaacgagaag attgtcagtc tcgcggtgga tgcacaatag
     2701 acgccgcgcc gtcgacaccg cgtacccccg aggccttatg gtccgtgatc ctgccaactg
     2761 gctgaccaag atgcgacctg cggtgctggc gttgagcgtc gcggcattgc cgcttgggta
     2821 tctcgatggt gcgacggctc acgcgcaaag catcatgcgc tcgcccaacc tcaacatcgg
     2881 ggcgcggatt ccgagcatca acccgacggt ggcgccgcgg atcaatccgg acatcgcggg
     2941 cagggtagtt tcgggagccg acggaaggcc gccgagagtc aacatcacct ccgtgacgag
     3001 atcgacgccg cggatcggtg tgacgtcgac gcttccttac gcgcgctatt cgcccaacct
     3061 ttatccggcc tgcagctacg cctatcgcga cagcgacggc gaatgccggg atcagcctgt
     3121 tacctcagcc gacggcggcg gcgccggcgg tgcgtcgggt aaaagcaaga acaacggcgc
     3181 gcgtcgcaac actaccttga cggctctcaa cctgcgcacg atcgcgaatg agctcgtggc
     3241 cgagatcgac ggttcgctgt ccgacgcgca ggccgatgaa ctggcgcggc gtcaccgcct
     3301 cgagcgcatc ggatcgcaga atttcccgct gataggcggc accatcggcc tgttccgcgt
     3361 cgtcgatcgc cgcacggtgg agaccgcgag ccgtgaactc gccactgacg ccagtgtccg
     3421 ctcggtgcag ccgaatttcc gctatgtgct gcaggaccag aaggttgcgc tgaccgaggg
     3481 cgaccccgcc caatacgcgc tccagaaact tcgcttgccc caggcccacg cgctggccca
     3541 tggcgccaac gtcaccattg ccgtgatcga ttccggtata gacgtcagac atccggaact
     3601 tgccaatgca gttgtggact ccttcgatgc actcgggagc agggaaggtc cgcatgttca
     3661 cggcaccggc attgccggcg cgatcgtgtc gcatgcgcgg ctgatgggaa gcgcgccagc
     3721 ggcgaggatt ttggcgatcc gcgccttcgg cgtggcggcg agcggcgcag caagcacgtc
     3781 attcgtgatc ctgaaggggc tcgattacgc cgcggcccac ggcgcccaga tcgtcaatat
     3841 gagcttcgct ggcccgaagg atggcttgat cgaacgcggc atcgccgccg ccgccgccaa
     3901 gggcatcttg atggtcgccg ccgccggcaa tggcgggccg aaatcgccgc cgctatatcc
     3961 cgccgccaat ccaaacgtga tggcggtcag cgccaccgac gcgcaggaca gattgttcgc
     4021 ggcgtccaat cgcggcagcc acatcgcgat agcggctccc ggcgtcgata tcttcctgcc
     4081 ggctccgaat gaaaaatacc agatgacgtc cggcacctcg ttttccgccg cttatatcag
     4141 cggtctcgcg gcgctgatgc tggagcggaa cccggtgttg aagcctgatc aagtgcgcgc
     4201 gattctggtg aagacagccc gcgatctcgg ctcccccggc cgcgacgatc tgttcggcgc
     4261 gggcgaagcc gatgcgtttg ccgcagtctc ggccgtcgtg accgcgccgg cggtgccggt
     4321 cgcggcggtt cccgacggcc atgccgctga aaaaatctcg ggccatcagg aggttcccgt
     4381 gaccagggac ttgaatcagc cgggtaccgc catggcttcc gacaaggcgg ctgttcgaga
     4441 agccaatcgg cccgccgcgc aataattttt cgtccgaaaa gacctgccaa aaaaagatct
     4501 gccaaaaagt taaaaaaatt gcaggccgtt ttgaacgttc ggcgaccctt cgcgaccaat
     4561 atatgtggga gcggtaaatc cctccccatc gaggctgtat tcggcgcaag cgcccatcca
     4621 ccccaagcgc ccatatagct tgacccgtcc ggttgtcccc ccggacgggt ctctcatttt
     4681 ggctttattt caggcgtttc cgcgcagtga aatcaccgct tgttccccag cccctcaatt
     4741 ttgtaatcgc tgcaacgccg ctgtgccgcg cgactgcagg caggatgaaa acctggcatt
     4801 tctgccgctc gctgccgatg ccgggcgctc ggccaaagat gatcgctgga caagacaaaa
     4861 gttttccaca gaatatcccg gtcacgtcta attgattcgt ctccgttgcg tctgcgtccg
     4921 ttcttttcct tacgggctga ttcatgactc atcgaacaga cgtggcacgc ggccgtgatg
     4981 tcgtcgcgcg ctggtgcgat cttgccgaac ggcggctgga atatctgacc gagctgttcg
     5041 aaaccggccg ctggcgccgc tactacagcg aacgcgcatt tctcgataat atccaggaag
     5101 ccaagaccgc ggtcgaaatc tggcgcgaat tgtcgacgcg cgaggcctcc agcgacaatt
     5161 tcgccgtcga catctcctgg cttggccgca cccgggccac cagggccatg ctgccgagcg
     5221 gtgaaacctc gcgcgaatgg gttcatcggt ttcagccgca gccgccgcag atcccggccg
     5281 aaccggtgcc gcgcgatgtc tcggtcgccg cggaacccgg tcatgtctgt tcggatgccg
     5341 cgccgtctgc accggagccg cctgcgccag ccctcgaccg atccttggac ccggtcctgg
     5401 acaggttgac acgcgacatt gccgcgatca cggaacgcta tccgctgctt cgcaatgtca
     5461 tgtaggcgac aacaacacca ttccgtcgcc tgtgttcgcg gggacgacag agggttaccg
     5521 tctcaccgca ttggtcccga tcaccacttg cgcaaaccgc tgcgcgccct cggcggaaag
     5581 atccggcgtg atggcgcgag cgtgatcgag cccgaccacg gcgccgcgcg gcgtttccga
     5641 gatcatgttg ttgttgatca aagccgtgcc cgcgcccggc accaccgaca cgccgacgcc
     5701 gacaaaggcg ttgcggatca cattgcccga tatcacgacg tcgcgcagat atttgcccca
     5761 gccggcgatg atcccgaacg ccggcgcatt ctcgatcaca ttgccggtga cggccgtatc
     5821 ggcctcgaca tagatgccga tgccggcgtc gtcgtccggc gcggtgccga tcggacgctt
     5881 cggcaggagg ttgcggatga tgttgccctg gaccaccgcg atgcgtccgc cgtcgttgaa
     5941 attgcagacc gaaacgccca gagccgcgcc gtcgacggtg ttgttggcga tcacggcgcc
     6001 ctcgaacgaa aactcggaat acagcgccac ttcgcggaca tcgctgacgc tattgcctga
     6061 tatctggatg ttcgatgccg aattgccacg caccgccgag tagtcgcaat tgctgatccg
     6121 gttgccgcgc acgatcacgt tgccggcgcg aaacgcgttg atggcgttgc cgtattggcc
     6181 ggagccgccg gggccggcct tgatgtccgc gatgcggttg tcgagcacct gggtgccatc
     6241 gtctccgaca gtgctgcgca ggatttcgat gccgttgtcg ttcgtccctt gaatcgggtt
     6301 gcgcgagacc agcaggccct gcgcatcgag cgacacgatc gcggtagtgg ctgacttggt
     6361 gatgatattg ccgctgatgt cgccggagac gttctcgaac cagatgccgt tgccgccaga
     6421 gccggcgatc tcacaatcgg taacgcggat gtcgcgtccg ccgaggcaat gcaccaggcc
     6481 gcgccgcacc ggcagagcaa tgccagcgcc atccagcgtg aggttggaaa gaccaacgct
     6541 gttggtgcct tcgccctgaa acatcgacgc gccgccggc
//
LOCUS       DASWGM010000201         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1260656,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000201 DASWGM010000000
VERSION     DASWGM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260656"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGA15_06080"
     CDS             <1..228
                     /locus_tag="VGA15_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HEX9587298.1"
                     /translation="RTASKGWATDLSDAAIVRSADEARALLAESVADDVGAVGAYIAP
                     VEIKDSGKIKPGNLREHIRSEGVTIDLPVPA"
     gene            241..1896
                     /locus_tag="VGA15_06085"
     CDS             241..1896
                     /locus_tag="VGA15_06085"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX9587299.1"
                     /translation="MYAYDEIDRTLINERVTEFRDQVKRRLSGELTEDEFKSLRLMNG
                     VYLQLHAYMFRVAIPYGTLSSEQMRRLAHVARRYDRGYGHFTTRQNIQFNWIKLAELP
                     DALADLAEVGIHAMQTSGNCTRNITADQWAGVAPGEIEDPRIWAELLRQYSTLHPEFS
                     FLPRKFKFAITASEHDRAAIKVHDIGLRLHKNADGETGFEVLVGGGLGRTPFIGKTIK
                     PFVPGRDLLSYVEAILRVYNQYGRRDNIYKARIKILVHELGIEKFAGEVEDEWLQMRD
                     SALTLDDADIEDIRSRFTYPAYEKLPHMPDELKKAAHDPHFEAWRKNSVAPHKVPGYA
                     IVTLSLKPVGGPPGDATADQMDAIADLADKYSFGEIRVGHEQNLVLPHVARRDLPALW
                     RALDKIGVATPNINLVTDIIACPGLDYCALANARSIPIAQELTRRFANHDTANLIGRL
                     HVNISGCINACGHHHVGHIGILGVEKNGEEFYQITIGGRADENAVVGSLIGPAVPYGE
                     VADVVEDIVEAYLALRARPDELFIDAVRRLGVEPFRERVYATR"
     gene            1883..2398
                     /locus_tag="VGA15_06090"
     CDS             1883..2398
                     /locus_tag="VGA15_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX9587300.1"
                     /translation="MPLVKNGKITGDAFVHVADDAPLPDDGAVLISAVRFLDEPEALL
                     HHVGKAGVIWPNNRDVDDLVPYLDRLAVVALVFPTFRDGRAYSQARLLRERFGYRGEL
                     RATGQVLRDQFVFMLRAGFDAFEVKKDSDAEAFASTAKRYSVFYQPTGDGRVTALHRR
                     MQLRHSESAGQ"
     gene            2395..3129
                     /locus_tag="VGA15_06095"
     CDS             2395..3129
                     /locus_tag="VGA15_06095"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX9587301.1"
                     /translation="MSALAHQVLPEVPTRDLSRAGELDRALRDASPAEVIAAALNAVG
                     REHLAVVSSFGIESAALLKVMADVDPAIPVVFLDTGWLFEETLAYRDTLIATLGLRDV
                     RSIKPLEEMLSREDGNRELWFTDPDACCRIRKVEPLARALAPFRAWINGRKRFQGGLR
                     ADIPVVEDDGARLKFNPFANVSREAIEAIYTDAKLPPHPLAASGYLSVGCMPCTSRTS
                     PDEDARAGRWRGRAKTECGIHTVKTS"
     gene            3255..4244
                     /locus_tag="VGA15_06100"
     CDS             3255..4244
                     /locus_tag="VGA15_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9587302.1"
                     /translation="MIRRILFVVAALLWASSAFAADVTLLNVSYDPTRELYVDFNKAF
                     AAAYQKETGKSVEIKQSHGGSGSQARAVIDGLQADVVTLALAYDIDAIAGKGLIPADW
                     QKRLPQNASPYTSTIVFLVRKGNPKAIRDWDDLIKSGVSVITPNPKTSGGARWNYLAA
                     WGYALKKFGTDDKAKQFVGDLYKNVPVLDTGARGSTVTFVERGVGDVLLAWENEAFLA
                     QREFGKDKFEIVSPPLSILAEPPVSVVDKVADKKGTRAVAEAYLKYWYTREGQEIAAR
                     NSYRPRDSEIAKEYENSFAKVELFTIDDVFGGWTKAQKEHFSEGGVFDQIYKN"
     gene            4272..>4393
                     /locus_tag="VGA15_06105"
     CDS             4272..>4393
                     /locus_tag="VGA15_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX9587303.1"
                     /translation="MSGGAGRRSTLPGFGLTMGLTLTWLSVIILIPLAGLFLKT"
BASE COUNT          852 a         1350 c         1371 g          820 t
ORIGIN      
        1 cgcacggcct ccaagggctg ggcgaccgat ctgtcagacg ccgcgatcgt ccggtccgcc
       61 gacgaagccc gtgcgctgct tgccgaatcc gtggccgacg atgtcggcgc ggtcggcgcc
      121 tatatcgcgc cggtcgaaat caaggactcc ggcaagatca agcccggcaa tcttcgcgaa
      181 catatccggt cggaaggcgt caccatcgat cttccggttc cggcgtaagg ttttcagctc
      241 atgtacgcat atgatgaaat cgaccgcacg ctgatcaacg aacgcgtcac cgaatttcgc
      301 gatcaggtga agcgccgtct gtccggcgaa ctcaccgagg acgaattcaa gtcgctgcgg
      361 ctgatgaacg gcgtctatct gcaactgcac gcctatatgt tccgcgttgc gatcccctac
      421 ggtacgctgt cctcggagca gatgcggcgg ctggcgcatg tggcgcggcg ctacgaccgc
      481 ggctacggcc acttcacgac gcggcagaac atccaattca actggatcaa gctcgccgaa
      541 ctgcccgatg cgctggccga tctcgccgag gtcggcatcc atgcgatgca gaccagcggc
      601 aattgcaccc gcaacatcac cgccgatcaa tgggccggcg tcgcaccggg cgagatcgag
      661 gatccgcgga tctgggccga gctgttgcgg caatattcga cgctgcaccc ggaattctcg
      721 ttcctgccac gcaagttcaa attcgccatc accgcatcgg aacatgatcg cgcggcgatc
      781 aaggtgcacg acatcggcct gcgcctgcac aagaacgccg atggtgaaac cggcttcgag
      841 gtgctggtcg gcggcgggct tggccgcacc ccgttcatcg gcaaaaccat aaaaccgttc
      901 gtgccgggcc gcgatctcct gagctacgtc gaggcgatcc tgcgcgtcta taaccagtac
      961 ggccgccgcg acaacatata caaggcccgc atcaagatcc tggtgcacga actcggcatc
     1021 gagaaattcg ctggagaagt cgaggacgag tggctgcaga tgcgcgacag cgcgctgacg
     1081 ctggatgacg ccgatatcga agatatccgt tcgcgcttca cctatccggc ttacgaaaaa
     1141 ctgccgcata tgccggacga gttgaagaag gcggcgcacg acccgcattt cgaggcatgg
     1201 cggaagaatt cggtcgcgcc gcacaaggtg ccgggctatg cgatcgtgac gctgtcgtta
     1261 aagccggtcg gtggtccgcc cggcgatgcc accgccgacc agatggatgc gatcgccgat
     1321 ctcgccgaca aatactcttt cggcgaaatt cgcgtcggtc acgagcagaa tctcgtgctg
     1381 ccgcatgtcg cgaggcgcga cctgccggcg ctgtggcgcg cgctggacaa gatcggcgtg
     1441 gcgacgccga acatcaatct ggtcaccgat atcatcgcct gcccgggact ggattactgc
     1501 gcgcttgcca acgcgcgctc gatcccgatc gcgcaggaat tgacgcggcg ctttgccaat
     1561 cacgacaccg ccaatctgat cgggcggctg cacgtcaata tttccggctg catcaacgcc
     1621 tgcggccatc atcacgtcgg ccacatcggc atcctgggcg tcgagaagaa cggcgaggaa
     1681 ttctatcaga tcacgatcgg cggccgcgcc gacgaaaacg ccgtggtcgg cagcttgatc
     1741 gggccggccg tgccctatgg cgaagtcgcg gacgtggtcg aggatattgt cgaggcctat
     1801 ctcgcgttgc gcgcgcggcc ggacgaactt ttcatcgatg cggtcaggcg gcttggcgtc
     1861 gaacctttca gggagcgcgt ttatgccact cgttaaaaac ggaaaaatca ccggcgacgc
     1921 gttcgtccat gtcgcggacg atgctccgct tccggacgat ggcgcggttc tgatttccgc
     1981 ggtgcgattt ctcgacgaac ctgaggcgtt gttgcaccat gtcggcaagg ccggcgtgat
     2041 ctggccgaat aatcgcgacg tcgacgacct cgtgccctat ctggaccggc tcgcggtggt
     2101 cgcgttggtg tttcccacct tccgcgatgg ccgcgcctac agccaggcgc ggctgttgcg
     2161 ggagcgtttt ggctatcgcg gcgagttgcg cgcgacgggg caggtgctgc gcgaccagtt
     2221 cgtgttcatg ctgcgcgccg gattcgacgc cttcgaagtg aaaaaagaca gcgacgcgga
     2281 ggcgtttgcg tcaaccgcaa agcgctattc ggtgttctac cagccaaccg gcgacggccg
     2341 cgtcaccgcg ctgcaccggc ggatgcagtt gcgtcattcg gaaagtgccg gccagtgagc
     2401 gctctagcac atcaggtatt gccggaagtt cctactcgcg atttgtcgcg ggcaggcgag
     2461 ctcgaccgcg cgcttcgtga cgcgtcgccg gcagaggtga tcgcggcggc gttgaacgcg
     2521 gtcggccgcg agcatctcgc cgtggtctcg tcgttcggca tcgaatcggc agcactgctc
     2581 aaggtgatgg ccgatgtcga tcccgcgatc cccgtggtct ttctcgacac cggctggctg
     2641 ttcgaggaga cgctcgccta ccgtgacacg ttgattgcga cgctgggtct gcgcgatgtc
     2701 cgttcgatca agccgctgga agagatgctg tcgcgcgagg atggaaatcg cgaattgtgg
     2761 ttcaccgatc ccgatgcctg ctgccggatc cggaaggtcg agccgctggc gcgggcattg
     2821 gcgccgttca gagcctggat caatggccgc aagcgttttc agggcggcct ccgcgccgac
     2881 attccggtgg tcgaagatga cggtgccagg ctgaaattca atcccttcgc caacgtctct
     2941 cgcgaagcaa tcgaggcgat ctacacggac gcaaaactgc cgccgcatcc gctcgcggca
     3001 tccggttatc tctcggtcgg gtgcatgccc tgcaccagcc ggacgtcgcc ggatgaagat
     3061 gcccgcgcgg gccgctggcg cggcagagcc aagacagaat gcggcatcca tacggtcaag
     3121 acttcctagg acaataacgc tgcgacaccg cgaacaatga aatgcggttt cgttgacttg
     3181 atgacgtttg gccccgagtt aagtgctccc tgctgtcgat gacaatgccg tcgtcggtgc
     3241 gaacggagat tgagatgatc cgccgtattc ttttcgtcgt cgcggcattg ctctgggcaa
     3301 gctcggcatt tgccgccgac gtgacgctgc tcaacgtgtc gtacgacccg acccgcgaac
     3361 tctatgtcga cttcaacaag gcgttcgccg ccgcctatca aaaggaaacc ggcaagagcg
     3421 tcgagatcaa gcagtcgcat ggcggctccg gctcgcaggc gcgcgcggtc atcgacggcc
     3481 tgcaggccga cgtcgtcacg ctggcgctgg cctacgatat cgacgccatc gccggcaaag
     3541 ggttgatccc cgcggactgg cagaagcggc tgccgcaaaa tgcctcgccc tacacgtcga
     3601 cgatcgtgtt cctggtgcgc aagggaaatc caaaagccat cagggattgg gacgatctga
     3661 tcaaatccgg cgtcagcgtc atcacgccga atccgaaaac ctccggcggc gcgcgctgga
     3721 attatctcgc cgcctggggc tatgcgctga agaaattcgg caccgacgac aaggccaagc
     3781 aattcgtcgg cgatctctac aagaacgtgc cggtgctgga caccggcgcg cgcggctcga
     3841 ccgtcacctt cgtggagcgc ggcgtcggcg acgtgctgct ggcatgggag aacgaggcgt
     3901 tcctcgccca gcgcgaattc ggcaaggaca agttcgagat cgtatcgccg ccattgtcga
     3961 ttcttgcgga accgccggtg tccgtcgtcg acaaggttgc cgataaaaaa ggcacccgcg
     4021 cggttgccga ggcttacctg aaatactggt ataccaggga aggtcaggaa attgccgcac
     4081 gcaattccta tcgtccgcgg gactcggaga ttgccaagga atacgagaat tcgtttgcga
     4141 aggtcgaatt gttcacgatc gacgacgttt tcggcggctg gaccaaggcg cagaaggaac
     4201 acttcagcga aggcggcgtt ttcgaccaga tttacaaaaa ctgacccgat ttcggggaag
     4261 cagagggcca tgtgagcgga ggcgcgggac gacgcagcac attgccgggg tttggtctca
     4321 ccatgggact gacgctgacc tggctctccg tcatcatcct gattccgctc gccggcctgt
     4381 ttctcaagac gtt
//
LOCUS       DASWGM010000202        26688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2647648,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000202 DASWGM010000000
VERSION     DASWGM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 26688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26688
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2647648"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VGA15_06110"
     CDS             complement(<1..497)
                     /locus_tag="VGA15_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587304.1"
                     /translation="MIEDGSGHIDYDESGEGPAVVLVPGSCSTGAAWRPVIAQWANGF
                     RCVTTSLLGYGGTVERRIGLDADIAHEAEVLEAVIRRAACPVHLVGHSFGGLTALAVA
                     LRGRVPLLSLTIAEAPAPEILRTMGEYRHYLAFRKMTEAYFRAFQAGETTAIERMIDF
                     YGGAGT"
     gene            complement(636..1478)
                     /locus_tag="VGA15_06115"
     CDS             complement(636..1478)
                     /locus_tag="VGA15_06115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9587305.1"
                     /translation="MSSSPAIQNAQDGRPDLAALKLRQLAAWSSGDYAVVGTTLQIVG
                     EELCEALDLRSGQKVLDVAAGNGNVTLAAARRWCDVVSTDYVPSLLERGRLRAAAEGL
                     SVEFKEADAEALGFADASFDIVVSTFGVMFAPDHDKAAAELVRVCKSGGKIGLANWTP
                     EGFIGQLFKVLGKYLPPPAGSKSPPLWGSRAHISEMFGAAALSIRAESRSFAFRYRSP
                     EHFVEIFKNYYGPTLKAFAALDEANQQGLRNDLLALIRRMNRAEDGTMVVPGEYLETV
                     ITKR"
     gene            complement(1545..2318)
                     /locus_tag="VGA15_06120"
     CDS             complement(1545..2318)
                     /locus_tag="VGA15_06120"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9587306.1"
                     /translation="MAGLFAVIYGIAAYATFLVTILYAIGFVGNWVVPKSIDSGTAGP
                     LIESVLVNTMLLGLFAVQHSVMARQGFKRWWTQFVPSSIERSTFVLFSSLALLLLYWQ
                     WRPIPEPIWMVRNPTIATALEAISLFGWGLLVVSTFMLSHFELFGLSQVFARLFGRNL
                     PAAKFHAPLLYRVVRHPIYLSFLLAFWATPLMTVGHLLFAVATTGYILIGIQLEERDL
                     IGMFGDQYRSYKERVSMLLPLPGRKFDDRTDTQPRSAER"
     gene            2708..4249
                     /locus_tag="VGA15_06125"
     CDS             2708..4249
                     /locus_tag="VGA15_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9587307.1"
                     /translation="MNINVRPHGSSSKPLLPRGVRRALDAMHANVERDWSVTELAGVA
                     GVSSRTLQRQFRIFLGKAPRTALRDIRFDSARRELLRGLPDAKVTNVALRYGLAHCGR
                     FSIEYRRRYGETPSQTLKRQAVFAGALTSMPSLLVSRGDRPTIALGPIDASPENRDLA
                     RSIADELATALTRAGVSVTNQPGSARYQLTSMIRGSDRQTRLTFRLIDAETGRHLSAH
                     RCDGALGGDAAPDEHLATRIVAALQPCLRLAEIDRAGRKPDADLSPHDLALRAMPCVL
                     SLNADGNARALDLLERAMERDPEHALATALAAWAHGQRVVYHFAATPDQDRARSGELA
                     RKAQTLAGDATVLAVLGNALTLLHDLDAADRVIRKALSIDGGSAWAWSRSGWIDVYNG
                     DAESAIERFKIALDLAPNDCLAFNSMVGIGCAHFETGRYLEAADWQQRALVEHPSASW
                     IHRTMCPAYVLIGAESEARRSVTALQEGYPGLTISDVQQGMPPLPEPYCDRVLDALHS
                     VGLPL"
     gene            complement(4474..5262)
                     /locus_tag="VGA15_06130"
     CDS             complement(4474..5262)
                     /locus_tag="VGA15_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9587308.1"
                     /translation="MVAIVGFSREQILSAVRKMYTDVATFPARQFHFPTGRAACVFVG
                     YPEDWLECIPATAVESFAGVGFPFRADVIRPGDRVLDVGAGSGTDTLTAARLVGPQGK
                     VFALDMTQAMLAKLRGNATQAEVKNVEVIEGNAETIPLPDASVDVVTSNGVLNLVPDK
                     PKVFAEIHRVLRPGGRVQIADIVVSQPVSESAKANPKLWAECVVGASVEQDYLGLFRT
                     AGFANVTVLRSFDYFSGSASADTRKIAAALGAKAVEITMERRNC"
     gene            complement(5274..5525)
                     /gene="rsrA"
                     /locus_tag="VGA15_06135"
     CDS             complement(5274..5525)
                     /gene="rsrA"
                     /locus_tag="VGA15_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEX9587309.1"
                     /translation="MSKDHIEHTPIDCEEVIAHLFAYLDNETDPEKRTDIERHLEECR
                     ACFSRAEFEKALRAKVNQLGDKEAPAMLRQRVAALLKQF"
     gene            complement(5536..6120)
                     /locus_tag="VGA15_06140"
     CDS             complement(5536..6120)
                     /locus_tag="VGA15_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9587310.1"
                     /translation="MTGDNDRDFFRAEVERLMDRLYGTALRLTRNPADAEDLVSEAVT
                     KAWANLGHLADRQAFQKWLFRILTNTFVSDRRRARSETSETIIGADGEETFSLFEKLH
                     QPFLLWWSNPEQELINTLLREDLERALDQLAEEYRTVVVLVEINGQSYAEVAEILGIP
                     IGTVRSRLSRARSTLQRALWKQAQAAGLQPDARS"
     gene            complement(6209..6808)
                     /locus_tag="VGA15_06145"
     CDS             complement(6209..6808)
                     /locus_tag="VGA15_06145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9587311.1"
                     /translation="MTVPDIFEGTGMPDPGWWDALWPEPAKVLSIVGVRSGTDVVDLC
                     SGDGWFTLQIAKLARHVIAIDVDATLLDTTRMRLAESGLTNCTIIQTDAYEVAKIIGN
                     PIDHVFLADVFHGVPDRPRLSKAVGSVLKPGGLFAIVNWHARSRLETTILGEPRGPAT
                     ELRMTPQATIAAVEPYGFKLRDVVEVGPYHYGAVFERRT"
     gene            complement(6884..7660)
                     /locus_tag="VGA15_06150"
     CDS             complement(6884..7660)
                     /locus_tag="VGA15_06150"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9587312.1"
                     /translation="MSNAVAFDPKELEAKVKAMYRSVAEEPHGEFHFEMGRAMAERLG
                     YAPADLDRIPAESIESFAGVGYYFHLAELKGGETVVDLGSGSGMDTFIAALKVGRAGK
                     VIGIDMTDEQRAKAERLRDRDDFLNVSYLKGYIEQVPLPDGVADVVISNGVVNLATEK
                     SSVFREAARLLKSGGRLALSDIVTEVQLPESIVCNSTLWAACIGGAAQHTNYRSQIEA
                     AGLRVVKMEDNPQYQFISDNAKGACKKFGVKSVSLLAQKS"
     gene            complement(7739..8107)
                     /locus_tag="VGA15_06155"
     CDS             complement(7739..8107)
                     /locus_tag="VGA15_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX9587313.1"
                     /translation="MAESTRELVVVVTQGMDHELSSVAFTIATGGITAGLQVSIFLTT
                     AAVDLARKRAIDGVVVHPLEPLAALVKDFQARGGRLWACTPCVKARGYEQSDLIDGVE
                     ITGASKMHELIKAGAATLSF"
     gene            complement(8818..8994)
                     /locus_tag="VGA15_06160"
     CDS             complement(8818..8994)
                     /locus_tag="VGA15_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587314.1"
                     /translation="MIDIRSGLLKALHDSRSLLAARLIDQHQHLVQDYRCIGVLHCAD
                     DAGLEPRQTELTEQ"
     gene            9481..10611
                     /locus_tag="VGA15_06165"
     CDS             9481..10611
                     /locus_tag="VGA15_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9587315.1"
                     /translation="MNDKEQFSALIGDIYDAALDPAQRIDVLEKIARFSGGQSGGLLS
                     KHSLGKSENLYCYVGADPDSLQVYSESYPKLDPTADLPYFGVQQVVSAAQFVPHDEFR
                     RGRFYREWARPHGWVDVASVVIEKSVTSCTFLSVVRHEASGLVDNEMRRRMALLVPHV
                     RRALLIGKTINLKQAQRVCFSDILDGLSAGMILVDVNGQIVHANAAGNAILEAADFLR
                     TVCGRLVAGDPAINTALREILVAANAGDAVLGVKGIALPLTAHDGVRYVAHVLPLTSG
                     ARRVACLAYNAVAALFIRKAALEAFAPPEVIGEMYKLTPTELRVLLAIVDIGGVPEVA
                     AALGVAVTTIKTHLSRLFEKTGVARQADLVKLVAGFSTPLAG"
     gene            complement(10690..11460)
                     /locus_tag="VGA15_06170"
     CDS             complement(10690..11460)
                     /locus_tag="VGA15_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587316.1"
                     /translation="MIHKTDAQLRRSSRTNTQPVALLNRRLLLAGPAAVLCTKALRAA
                     APEQRPLVLSLADAAQVRPRNVSVAPASFNGRSCVEVRLAGAYPGPDRDYFAYVPGLD
                     MHDGTIEVELAGAPLPSAPAGARGFAGLAFRIDEAGGSFACEGIYLRPTNGRADDQVR
                     RNHATQYFSYPGYDFDRLRREAPARYESYVDVVPAQWIRIRVELSGSRADLFVDDATQ
                     PVLVVTDLKRGAEARGTVGLYVDNGTDGHFCNLRIIPR"
     gene            12135..12821
                     /locus_tag="VGA15_06175"
     CDS             12135..12821
                     /locus_tag="VGA15_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9587317.1"
                     /translation="MKSKTSIKRRSRAVEAEDGTLTDRAYRELEEMIVTLQLLPGTVL
                     SEQALAVRLKIGRTPIREALQRLARDGLVVIMPRRGIMVSEINLRLQLRLLEVRRELE
                     RLMASLAAERATADERREFAEVAEAMLVAAAKSDDIAFMRLDQRFNILIATAARNEFA
                     RRSMGLMNALSRRFWYQHYQEVADLPLAAKLHAAVAEAVSQKKIKAAATASDRLIDYI
                     EDFARKTIDT"
     gene            complement(12929..13942)
                     /locus_tag="VGA15_06180"
     CDS             complement(12929..13942)
                     /locus_tag="VGA15_06180"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9587318.1"
                     /translation="MVLRYRAAVLHAPQTPLSVETVTAASLKPTDVLVRIRAAGLCHT
                     DLEVIEGSLRYPLPIVLGHEAAGVVEQAGTAARGVKIGDHVVLSWNPHCGHCFYCDRD
                     SPILCEEYLGGGPQGRAFDGESRAMLADGRELQQLMFLGSFGEYCIVRDQQAIPIPKE
                     VPFDRACLIGCGVMTGVGAALNLGAIAHGDTVMVIGCGAVGLAAVQGARLAGAGAIIA
                     VYLDPAKLALAARMGATHGVNASADDVIAAGRRETGGRGVDVVIESAGSASAFRTTPE
                     AVRPGGQVIWLGKIDVNQDVSFRWGSLMQEKAHPPRQLRQRAAAPRFSAARASLSRRL
                     PVA"
     gene            complement(13943..14251)
                     /locus_tag="VGA15_06185"
     CDS             complement(13943..14251)
                     /locus_tag="VGA15_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6166 domain-containing protein"
                     /protein_id="HEX9587319.1"
                     /translation="MKHYLGDRTIDGVKVTVDGAPLDPRIDVMEFTRNGFEWSYEGPE
                     PRQLALALLVDHLGDNAAAMTAVETFMRAIVANFENEWEMTSDDIDLALTALDGKAVA
                     "
     gene            complement(14291..15010)
                     /locus_tag="VGA15_06190"
     CDS             complement(14291..15010)
                     /locus_tag="VGA15_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9587320.1"
                     /translation="MLSLSNLSVDIEGSRILTDISLNVAAGELVCLVGRNGAGKTTTF
                     RSIMGYRKAVSGDIIFEGADLTKLPTWQIAQKGIGFSPEESEVYADLTVAENIELSTW
                     TRPSGRSAGIRIEEAYSVFPKLRQYLARGGAQLSGGERKMVSIARALALDPKLLLLDE
                     PFEGLSPAIIPMIAKGVASIRAMGRGVLIAESNVHHIPDYADRIYVLERGEVMFAGTL
                     EEAYRDRDVMRVIAGKIEAAS"
     gene            complement(15069..15866)
                     /locus_tag="VGA15_06195"
     CDS             complement(15069..15866)
                     /locus_tag="VGA15_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587321.1"
                     /translation="MQPNHAPAADAAPLFETVNVSKSFGAFKALQNISFQVSDGEFVS
                     IVGPNGAGKTTLVNVVTGLLRPTIGEVRFLGRDIAGIGPVELARLGMSRAFQLVNIFP
                     ALTVRETLAVAVASRLRRASNPFRSLTRDAVLQADVERVAEVLGLRAKLDIVASTLSQ
                     GEKKLLDIASAFALNPTVILLDEPTSGVSSGDKHAIMEVLVTAARAAGVRAIVQVEHD
                     MDLVERYSHRIVALQGGTLLADMKPDIFFADPAMIAAVVGIRPRLLR"
     gene            complement(15869..16882)
                     /locus_tag="VGA15_06200"
     CDS             complement(15869..16882)
                     /locus_tag="VGA15_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587322.1"
                     /translation="MTHPSIDIASPATVRRTVPRLNGLWVLLGLIVVMALLPAVASPY
                     ALLLLLPFIGYGIALLGFNLLFGTTGLLSFGHALFLAVGAYVAAVLTSKFGILSFEVL
                     LITAALVSGLVSLVIGVLCVHYTKIFFGMLTLAFGMLFHSFLFKFYSITGGDQGMRVL
                     RPLLLGMEWRGGKTAFLTGPFYYYALVLFALLGLLMWRITRSPFGLHLRAIRENAGKA
                     AYVGVEIFRMRLFAFVISAIYGGIGGTILAITTGLADPELAYWTHSGNLVFMAVLGGS
                     GTFAGPAIGALAFVILQDFVMSITQYWRFVMGGVLVLLVVFMPQGLSGTIELLAHRRN
                     GGR"
     gene            complement(16879..17760)
                     /locus_tag="VGA15_06205"
     CDS             complement(16879..17760)
                     /locus_tag="VGA15_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587323.1"
                     /translation="MTEFVDVVLGGTFHAAVLFLVAAGLQLVFGVQKIVNLACGSFYA
                     LGAYFGITAVGIAIKLGMPPLLFFPALIIAGLAIGLVGLPIERVLRTVYRRDESYQLL
                     ITFGFLLMFQDAFRYLWGATPRTMDNVYLACGTAHIWGVRVPTYNLLVIAASLAIAAA
                     LGLFLERTKTGRIVRATAENRDMAEGLGVNASKIFALVFTVGCMLGTVGGALVVPSGA
                     ASLDMAVELVVESFAVVVIGGLGSMRGALVGALVVGLMRAAAISLMPEMEMLSVYLVV
                     VVVLILRPAGLFGKATA"
     gene            complement(17763..19037)
                     /locus_tag="VGA15_06210"
     CDS             complement(17763..19037)
                     /locus_tag="VGA15_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587324.1"
                     /translation="MKVTRRDFSRLAGTGVLAAAAPGLFAPALAQDKPLRIGIIAPRS
                     GAAGTAGECGIRAVQWAAERMNRDGGIAGRQIELVFEEETNPKDTIERFRRLILQEKV
                     EAVHGLISTGVSLGVAPVAEEEQALLVMWDGTTQDGVKETMPNSRYVFRSTDNECEAV
                     MGSLLAIKYFKGKFKRVAGINPDYSYGRNNWEAFRQILARYGIEAEFVAEQWPKVGTM
                     DLTSHIAALNAAKPDLIFSSMLFADLPVFMKQGHAAGLFDGVKMVLPAVGWQINQLKK
                     EFMPEGVIFGHNTLYFDHPQASALQKAFVADYMDRYKEAPHWEADRAYFALATYKAGV
                     EAAQKAAGKWPTPEQVAQAMPGLEVESLGGKGRFRKDKIAEQVFYQGPSTNTNKYDFP
                     TLASIDTFQAEQLQKPPGADFWEWIKSAKMPV"
     gene            19226..20029
                     /locus_tag="VGA15_06215"
                     /pseudo
     CDS             19226..20029
                     /locus_tag="VGA15_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019491.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
     gene            20064..20462
                     /locus_tag="VGA15_06220"
     CDS             20064..20462
                     /locus_tag="VGA15_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587325.1"
                     /translation="MDAIEPHSISLLWFTAFATVCAVAFLVVAGMFPLRSRPDSLKSG
                     VATLLVVSNVVLLVVLLTGTGLYGYAELRRSTLVVVAGLVVLFAPGLFEVWPSSLRDG
                     SAGLTALAGIQMLALAALAKLGGLAWASMS"
     gene            20482..20691
                     /locus_tag="VGA15_06225"
     CDS             20482..20691
                     /locus_tag="VGA15_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587326.1"
                     /translation="MPVKIKLGLSLLVVLVAAIASYYQHALGHDVVQYVVAFLGFFMI
                     FAMWLFPEVKREESAGGVRRDTLPR"
     gene            complement(20774..21112)
                     /locus_tag="VGA15_06230"
     CDS             complement(20774..21112)
                     /locus_tag="VGA15_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX9587327.1"
                     /translation="MPVYEYLCNDCGPFTDMRPMAECDAPQDCPRCELEAPRVILTAP
                     NFFCMPSDKRKANATNERSANAPKTVGEYKASHGPGCGCCSTKPSRLVKQTKSGAKSF
                     PTARPWMISH"
     gene            complement(21290..22519)
                     /gene="fmdA"
                     /locus_tag="VGA15_06235"
     CDS             complement(21290..22519)
                     /gene="fmdA"
                     /locus_tag="VGA15_06235"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HEX9587328.1"
                     /translation="MPETLIKVDLTQSAYENDMIHNRWHPDVPMVAWVNPGDDFIVET
                     YDWTGGFIKNNDSADDVRDIDLSIVHFLSGPIGVKGAEPGDLLVVDLLDVGPMKESLW
                     GFNGFFSKQNGGGFLTDHFPLAQKSIWDFHGMYTSSRHVPGVNFAGLIHPGLIGCLPD
                     PKLLATWNERETALIATNPTRVPGLANPPFGPTAHMGRLKGEAKAKAAAEGARTVPPR
                     EHGGNCDIKDLSRGSKVYFPVYVPGGGLSMGDLHFSQGDGEITFCGAIEMAGWLHLKV
                     DIIKDGVNKYGIKNPVFKPSPVTPNYKDYLIFEGISVDEQGKQHYLDVHIAYRQACLN
                     AIEYLKKFGYSGAQAYSILGTAPCQGHISGVVDVPNACATLWLPTEIFDFDIMPSSAG
                     PIKHIKGDIQMPLSQDK"
     gene            complement(22555..23244)
                     /gene="urtE"
                     /locus_tag="VGA15_06240"
     CDS             complement(22555..23244)
                     /gene="urtE"
                     /locus_tag="VGA15_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEX9587329.1"
                     /translation="MLAISDLHVAYGQSEVLHGLNVSVAPNEIIAIMGRNGMGKTTLM
                     KSLMGIVPTKSGSVTMEGAELSTLKSYERVAKGLAYVPQGRMIFSAMTVKENIETGLV
                     ASGETEVPGDIYELFPVLLEMKSRRGGNLSGGQQQQLAIARALATKPKVLLLDEPTEG
                     IQPSIIREMARTLKRIRDERGLSIVVSEQVLSFALDIADRVLVIENGEIVRDDAREGV
                     DAAQISKYLSV"
     gene            complement(23313..24068)
                     /gene="urtD"
                     /locus_tag="VGA15_06245"
     CDS             complement(23313..24068)
                     /gene="urtD"
                     /locus_tag="VGA15_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX9587330.1"
                     /translation="MLIGHQPKAFLLSVEALTVSFDGFKAVNDLSFYVDENEIRVIIG
                     PNGAGKTTVLDLICGKTKATSGSIHFRDKDLTKMKENQIVQAGVGRKFQTPSIYDDLT
                     VFENLEISFPRGRTVFGALTFTRDAAVRDRVHEVAEMIFLKDRLNVKAELLSHGQKQW
                     LEIGMLLIQDPELLMLDEPVAGMSVSERVKTAELLNRIIKDRSVLVIEHDMKFVEDIA
                     HKVTVLHQGQILSEGSMEHVKNDPKVIEVYLGH"
     gene            complement(24080..25225)
                     /gene="urtC"
                     /locus_tag="VGA15_06250"
     CDS             complement(24080..25225)
                     /gene="urtC"
                     /locus_tag="VGA15_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX9587331.1"
                     /translation="MTDNRFVNRSELIGILVLAVFLVVILPLCLDVFRLNLVAKYLTY
                     AFVALGLVICWGYGGILSLGQGVFFGLGGYCMAMFLKLEASSPENTKIQSTPGIPDFM
                     DWNQITSLPLFWKPFHSLTFTIAAVILVPGIFALIIGAAMFKRRVGGTYFAIITQAVA
                     AILTILIVGQQGYTGGINGMTDLRTLKGWDIRPDHAKIVLYFFEVGCLFVCIFIAQFI
                     RHSKLGRILVAMRDKEDRVRFSGYSVANFKIFAFCVAAIFAAIGGAMFALEVGFMSPS
                     FVGIVPSIEMVIYTAVGGRLSILGAVYGTLLVNFAKTSLSESFPELWLFGLGGLFIAV
                     VLAFPNGLAGIWSDYVQPHLDRLISSRKSKSDDGWTDKSVADGAPAE"
     gene            complement(25243..26169)
                     /gene="urtB"
                     /locus_tag="VGA15_06255"
     CDS             complement(25243..26169)
                     /gene="urtB"
                     /locus_tag="VGA15_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX9587332.1"
                     /translation="MFGDYSIGDLGSIFVMQGFAGLILFSVYVLMALGLAIIFGQMGV
                     INMAHGEFMILGAYVTWMTSNLFQSYLPGLFAGYFFLAMILAFLASGALGMLVEWVLI
                     RHLYKRPLDTLLATWGLSLILQQAYRSIFGAREVGVELPQWMLGSLHVSDSIEVPING
                     VFVMCLTVLITLCVAYIMYKSRWGRQVRAVVQNRVMAGAVGINTEKVDRYTFGLGCGI
                     AGVAGSAFTMIGSTGPTSGQLYIVDTFLVVVFGGAASLIGTIASAFSISQTQSTLEFF
                     MSGSMAKVLTLLAVVGILMLRPQGLFALKVRK"
     gene            complement(26314..>26688)
                     /locus_tag="VGA15_06260"
     CDS             complement(26314..>26688)
                     /locus_tag="VGA15_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEX9587333.1"
                     /translation="AGAYACMKYFQSLDNPNNKAFVPAFKKMWGEKTVIGDVTQAAYL
                     GPWLWKLTAEKAGSFDIDKIAAASPGIEFKGAPEGYVRIHENHHLWSKTRVGRAKLDG
                     QFELIYETADLVEPDPFPKGYQ"
BASE COUNT         5365 a         8289 c         7887 g         5147 t
ORIGIN      
        1 gtgccggcgc caccatagaa gtcgatcatc cgttcgatcg cggtcgtctc gccggcctgg
       61 aatgccctga aataggcttc ggtcatcttg cggaatgcga gatagtgccg atactcgccc
      121 atcgtccgca atatttccgg cgcaggcgct tcggcgatcg tcaggctcag caacggcacc
      181 cgccctctca acgcgacggc gagcgcggtc aggccgccga acgaatggcc gacgagatgc
      241 accgggcagg ccgcgcgccg gatcaccgct tcaaggacct ccgcctcgtg agcaatatcg
      301 gcatcgagac cgatacggcg ttcgacagtg ccgccataac cgagcagact cgtggtgaca
      361 cagcggaaac cgttcgccca ttgtgcaatg acgggccgcc atgccgcgcc ggtgctgcat
      421 gatcccggca caagcaccac ggcagggccc tccccgcttt cgtcatagtc gatgtgcccg
      481 gacccgtctt cgatcatggt gtcagcgttc cttcagcgca gacaaggtta gcgaaagttg
      541 cgccagacga cgacctccga tcggcgaacc aatcgatcaa tcgatcgatc atccgacgga
      601 cggcggcgcg aacgtcctcg cccaagagag tgtgatcagc gctttgtgat cacggtttca
      661 aggtattcgc ccggaacgac cattgtaccg tcttcagcgc ggttcatccg cctgatcagc
      721 gcgagcagat cgtttcttaa tccctgctgg ttcgcctcgt cgagcgcggc aaaggctttc
      781 agcgtcggcc cgtaatagtt cttgaagatt tcgacgaaat gctctggcga tcggtagcgg
      841 aacgcgaaac tgcgtgactc ggccctgatg gaaagtgccg ccgctccgaa catctcgctg
      901 atatgcgccc tcgagcccca cagcggcggc gacttcgaac cggccggcgg cggcaggtac
      961 ttgccaagca ccttgaagag ctgcccgata aagccttccg gcgtccaatt ggccaagccg
     1021 atcttgccgc cgctcttgca taccctcaca agctcggctg ccgccttgtc atgatcgggc
     1081 gcgaacatga cgccgaaggt cgacacgaca atgtcgaagc tggcgtcggc aaagcccagc
     1141 gcttccgcgt cggcctcttt gaattcgacc gacaagcctt cggccgcggc gcgcagtcgg
     1201 ccgcgctcca gcaggctcgg cacatagtcg gtggacacga cgtcgcacca gcggcgcgct
     1261 gcggccagcg tgacattgcc gtttccggcg gcgacgtcga gaaccttctg tcccgagcgc
     1321 agatcgaggg cttcgcacag ttcttcaccg acgatctgca gcgtcgtgcc gacgacggca
     1381 tagtcgccgg acgaccacgc ggcaagctgg cgaagcttca acgctgcgag atcgggccgg
     1441 ccgtcctggg cgttctggat ggcgggagat gatgacatca ttgttccatt caaatttgtc
     1501 ggtttcgttg cttttcgctc ggcctgctgc cggcgccgtg accctcaacg ctcggcagac
     1561 cgaggctgcg tgtccgtccg atcgtcgaac ttgcgaccag gcaagggaag cagcatcgaa
     1621 acccgttctt tgtagctgcg atactgatcg ccgaacattc caatcagatc gcgctcttcg
     1681 agctggatgc cgatcagaat gtaaccggtc gtcgccaccg cgaacagcag gtggccgacc
     1741 gtcatcaatg gcgtcgccca gaaggcaagc agaaagctca gatagatcgg atgccgcacc
     1801 acccgataca gcagcggcgc gtgaaatttt gccgccggca gatttctccc gaacaggcga
     1861 gcgaacacct ggctgaggcc gaatagctcg aaatggctga gcatgaaggt gctgacaacc
     1921 agcaggcccc agccgaacag ggaaatcgct tcaagggcgg tcgcaatggt cggattcctc
     1981 accatccaga tcggttcagg aatcggccgc cactgccagt acagcagcag cagcgcgaga
     2041 ctggagaaca gcacgaatgt gctgcgttcg atcgaggacg gcacgaactg cgtccaccag
     2101 cgcttgaaac cctgccgcgc catcacgctg tgttgtacag cgaacaggcc gagcagcatg
     2161 gtattgacga gcacgctctc gatcagcggc ccggccgtcc ccgagtcgat cgacttcgga
     2221 accacccaat tgccgacgaa gccgatcgcg tagaggatgg tgacgagaaa cgtggcatag
     2281 gcggcgatgc catatatcac tgcgaacaag cctgccatta aatgctcctg ttgaaactga
     2341 tttgaatttt cactcatttg aatgccttgc gtggccgaga cttaataccg agctctttat
     2401 accgtgctct ttcttgtccc tatccgattt gcgacccgac catccgcggt gcgaaattct
     2461 tcgcgggcct taacgccggt cgtatcggcg acccgtcgag cccgcggctc ccgagccgag
     2521 agccgtgggc agcgcgacaa cggtgaaatt tccaccgcca atcaaccgaa cggaggatgc
     2581 tggcgtgctt ccaaagcggt ttaactggaa gttccgaaac gtcctatcca atttgcgaca
     2641 gcgctatccc tttgatgaag tttttcgacg tatttgcgtt taatggtcgg cttgccggag
     2701 tgtcccaatg aatatcaatg tacgccccca cggctcttcg agcaaacctc tgctgccgcg
     2761 cggcgtccgc cgcgcgctcg atgcgatgca tgcaaatgtc gagcgcgact ggagcgtgac
     2821 cgagctggcc ggcgtcgctg gcgtatccag tcgaacactg caacggcaat ttcggatctt
     2881 tctcggaaaa gcgccgcgta cggcacttcg cgatatcaga ttcgacagcg cccgccgcga
     2941 actgctgcgg ggcctgcccg atgccaaagt gacgaatgtc gcgctgcgct acggtttggc
     3001 gcattgcggg cggttttcca tcgagtaccg tcgccgctac ggcgaaacac cgtcgcagac
     3061 cttgaagcgg caagctgtct tcgccggtgc gttaacgtca atgccctcgc tgctcgtctc
     3121 ccgtggcgat cggccgacca tcgcgctggg tccgatcgat gcgagtccgg agaaccgcga
     3181 cctcgcgcgc agcatagcgg atgagctcgc aaccgctctc acgcgggccg gcgtctccgt
     3241 gaccaaccag cccggctcgg cgcgctacca gttgaccagc atgatccgcg gatcggaccg
     3301 gcaaacacgc ctgaccttcc ggttgatcga tgcggaaacc ggacgtcatc tgtcggcgca
     3361 tcgctgcgat ggcgcgctcg ggggcgacgc ggctcccgac gagcatctcg ctacccgaat
     3421 cgtcgcggcg ctgcagccat gcttgcgcct ggcggaaatc gatcgcgccg gccgcaagcc
     3481 ggacgccgat ctgagcccac acgatctggc gctgcgggcg atgccttgcg ttctctcgct
     3541 caacgccgac ggcaatgctc gcgcgctgga cctgctggaa cgggcgatgg agcgcgaccc
     3601 cgagcatgcg ctggcgacgg cgctcgccgc ctgggcacat gggcagcgcg tcgtttatca
     3661 tttcgccgcc acgccggacc aggatcgcgc ccgaagcggc gaactggccc gaaaggcgca
     3721 gactctcgcc ggagacgcga ccgtgcttgc cgttctcggc aacgccctca ccctcctgca
     3781 cgacctcgac gcagcggatc gagtgatccg caaggcgctt tcgatcgacg gcggctcggc
     3841 ctgggcgtgg agccgcagcg gatggatcga tgtatacaat ggcgatgccg aatcggccat
     3901 cgaacggttc aagattgcgc tcgatctcgc tcccaacgac tgcctcgctt tcaacagcat
     3961 ggtcggaatc ggctgcgccc attttgagac cggccgctat ctcgaagccg cggattggca
     4021 gcagcgcgcg ctggtcgagc atccgtcggc aagctggatc catcgcacca tgtgtcccgc
     4081 ttatgtgctg attggagccg aatccgaagc ccgtcgcagc gtcacggccc tgcaggaagg
     4141 ctatccgggg ctgaccatct ccgacgtgca gcaaggcatg ccgccgctgc cggagcccta
     4201 ttgcgatcgc gtgctagatg cgctgcacag cgtcgggctg ccgttgtaag gctgcgctcg
     4261 cgaagggacc tagtggatca aatctgacgc ttggtacccg ccatgggagc tctcgcgctc
     4321 ctttgctgtt ggagcaactt gggcacatcg cctcagttgt tgcagcgcga tattactgca
     4381 gcgattgggg gaatagcgga catgacgcgg acaacgctga accgacgcgc cccagagcgg
     4441 accagcgcag gggtgtattc tgcattgcaa gaattagcag ttccgtctct ccattgtgat
     4501 ctcgacagct ttcgcaccca gcgccgccgc gatttttcgg gtgtcggcgc ttgcgctacc
     4561 ggaaaagtag tcgaaactgc gcagtaccgt cacattggca aaaccggccg ttcggaacag
     4621 gccgaggtaa tcctgctcga ccgaggcacc gacgacgcac tctgcccaga gcttcggatt
     4681 agctttggcg ctctcgctga ccggctgcga tacgacgatg tctgcgatct gcactcggcc
     4741 tcccgggcgt aacacgcggt ggatttccgc gaagactttc ggtttgtccg gcaccaggtt
     4801 gagcacaccg ttgctggtga ccacgtcgac gcttgcgtcg ggcaggggga tcgtctcggc
     4861 gttgccctcg atcacctcca cgttcttgac ctcggcctgg gtggcattgc cgcgcagctt
     4921 cgcgagcatc gcttgcgtca tgtcgagcgc aaataccttg ccttgaggac cgacgagccg
     4981 ggctgccgtc aaagtgtcgg taccggaacc cgcgccgacg tcgagcacgc ggtcgccggg
     5041 ccggatcacg tcggcacgga acggaaagcc aacgccggcg aacgactcca ccgcggtcgc
     5101 cggaatgcac tcgagccagt cctccggata gccgacaaac acgcatgcgg cacggccggt
     5161 aggaaaatgg aactgcctgg cgggaaacgt cgcaacatcg gtgtacattt tccgcacagc
     5221 gctgagtatt tgttcccgcg agaatccgac aatcgccacc atgactggcc ccctcagaat
     5281 tgtttgagca aagccgccac gcgctgccgc agcatggcag gggcttcctt gtcgccgagc
     5341 tgatttacct tcgcccgcag cgccttctcg aactcggccc gcgaaaagca ggcgcggcat
     5401 tcctcgagat gacgttcgat gtccgtgcgc ttttccgggt cggtttcgtt gtccagatac
     5461 gcgaacagat gcgcgatcac ttcctcgcag tcgatgggcg tgtgttcaat gtgatccttg
     5521 ctcatggaca tgttcctaac ttcgcgcgtc aggctgcagg ccggcggctt gggcctgttt
     5581 ccacagcgca cgttgcaggg tcgatcgggc gcggcttaag cgtgaacgca cagttccaat
     5641 cggaatgccg agtatctcag ccacttccgc ataggactgg ccattgatct ccaccaggac
     5701 gacgacggtc cggtattcct ctgcgagttg atccagcgcg cgctccaaat cttcccgcag
     5761 cagcgtattg atcagctctt gttcaggatt gctccaccac agtagaaacg gctggtgcag
     5821 cttctcgaac agcgagaacg tttcctctcc gtcagcaccg atgatggttt cgctcgtctc
     5881 ggagcgggca cggcgccggt ccgagacgaa ggtatttgtg agaattcgga acagccattt
     5941 ctggaacgct tgccggtcgg ccagatgacc taagttcgcc caagctttgg tcaccgcctc
     6001 cgagacgagg tcctcggcgt cggccgggtt gcgcgtcagg cgcagcgccg tgccatagag
     6061 acggtccatc aaccgctcga cctcggctcg aaagaaatcg cgatcattgt ccccagtcat
     6121 ggcgaacttt gacgctccgg ccaccggaaa attctaaagg ccatataacg cttcgagaag
     6181 ccatacacaa atctgcaacc ggcaacgctc acgtccgacg ctcgaatacg gctccgtaat
     6241 gataaggccc aacctcgacg acgtcgcgaa gcttgaaccc gtaaggttca accgccgcga
     6301 ttgtcgcctg cggcgtcatc ctgagctctg tggccggtcc cctcggctca ccgaggatgg
     6361 tggtctccag acgcgagcgc gcgtgccaat tcacgatcgc gaatagtccg cctggcttca
     6421 agacgcttcc gacggccttc gacaggcgcg gcctgtccgg aaccccgtgg aaaacatccg
     6481 ccaagaaaac gtggtcgatc ggattgccga tgatcttggc gacctcgtag gcgtccgtct
     6541 ggataatcgt gcaattggtc aaaccgcttt cggcaaggcg catccgcgtg gtgtccagca
     6601 gggtagcgtc gacgtcgatg gcgatgacgt ggcgagcaag cttcgctatc tgcagcgtaa
     6661 accagccatc tcccgagcac agatccacga catccgttcc agaccgaact ccgacgatcg
     6721 aaagcacttt agcgggctca ggccatagcg catcccacca gcccggatca ggcatgccgg
     6781 tcccttcgaa gatgtcggga acggtcattt ttcgatgtga actccctgcg ccagcggcgg
     6841 cgctcacgtt cggttgcggg agttgtgcag gtgctgacgc cattcaggac ttttgcgcca
     6901 gcagcgagac gctcttgacg ccgaatttct tgcaggcgcc tttggcgttg tccgaaatga
     6961 actggtactg cggattgtct tccatcttga cgacgcgcag tcccgccgcc tcgatctggc
     7021 tgcgatagtt ggtgtgctgc gccgcgccac cgatgcaggc ggcccacagg gtcgagttgc
     7081 acacaatgct ctctggcaac tgcacctcgg tgacgatgtc ggaaagcgcg agcctgccgc
     7141 cagacttcag gagtcgcgcc gcttcgcgaa acacgctgct cttctccgtc gcaaggttga
     7201 cgacgccgtt gctgatcacg acatcggcga cgccgtccgg cagcggcact tgttcgatgt
     7261 aacccttcaa gtagctcaca ttgaggaagt cgtcccggtc gcgcagccgt tcggccttgg
     7321 cgcgctgctc gtccgtcatg tcgatgccga tcaccttgcc ggcgcggccg accttcagcg
     7381 ccgcgatgaa agtatccatc ccggacccgc tgccgagatc gacgacggtc tcgccccctt
     7441 tgagttcggc cagatggaaa taatagccga ccccggcaaa tgattcgatt gactcggcag
     7501 gaatgcggtc gagatcagcg ggcgcgtagc cgagtcgctc ggccatcgcg cggcccattt
     7561 cgaaatggaa ttctccgtgc ggctcttcgg cgacgctgcg gtacatcgcc ttcaccttgg
     7621 cttcgagttc ctttggatcg aaagcaacag cgttggacat gttcttctcc tggaattggt
     7681 gatcatctgt cgttgcatga gcgccggaag cgcaccgatg ctggaagcgg cggcggttct
     7741 agaagctgag ggtcgccgcg ccggccttga tcaactcgtg catcttgctg gcgccggtga
     7801 tctcgacccc gtcgatgagg tcgctctgtt cgtatccgcg cgccttgacg catggcgtgc
     7861 acgcccacaa gcggccgccg cgagcttgaa aatccttaac cagggcggcg agcggctcca
     7921 gcgggtggac cacgacaccg tcgatggccc gcttgcgcgc gagatcgacg gcggcggtgg
     7981 tcaggaagat cgaaacttgc aacccggcgg tgatgccgcc agtggcgatg gtgaaggcaa
     8041 ccgaggatag ctcgtggtcc attccttggg tgacgaccac gacaagctca cgggtagatt
     8101 cggccatttt tttccccttg cgcctcttgt tgcgggaggt tggatgcaat cctccgttgc
     8161 tcaggcagag ctaaggacgc aacacggtcc gaattagttc ccgactattt ctcctgcaag
     8221 ctttgaatgt ccgttggcac atagcgtcag ttagtgcggt gcagcgatat gtccggagtt
     8281 gggagtaaac cggactcgtg accgacggtt tcgatccgac gctccaagct taagccgacg
     8341 ttcatgcctg atccatttcg tgtgtcggag tgggtaccaa gcgtcagatt tcatccacca
     8401 ggcaagataa ggtctgtttt tgcccaatgt cgcaggcacc tgcgatcggg accaaagccg
     8461 aaccgcctcg aagcgcgcga attggccgcc cataccgtaa ccggcggacg tgccggccat
     8521 cgccgggctg gagaagaaca gcagcggctg caatgaattt tctcgccgcg ggttattcct
     8581 ccagctcggt ggcgtacaac ggtgacgcca cctcggcgcg gccggcaccc gccacaggcg
     8641 tcgccctggt ctcggcaacg cacctcaagc aatcaaatct agtctgaata gacatcgagg
     8701 accgcagctg gcctctcgct ctgcattgca ttcaatttct gcaaggcgag tatgtgggca
     8761 aatgcaaaga caatcattaa aacagccagc atgaaatgaa ttcgccacgc catgaaatca
     8821 ttgctccgtc agttcagttt gccgcggctc aagtcccgca tcgtcagcgc aatgaagaac
     8881 gccgatacat cgatagtctt gaacaaggtg ctgatgctga tcgatcaagc gagccgccaa
     8941 caggcttcgc gagtcatgta gcgccttgag caggccgctg cggatatcga tcatgccggg
     9001 cgcgaggacc cgcgcgaaat tgcgcaaccg ggaatacagt cgtcgccttt tccaattcca
     9061 tcgcacggca ctaacgatcg gggcggacaa tcgcgccgct tcggttctaa atgcgtgcat
     9121 cgaacctgcc ttgctatgtt ttgcgtgcgc gtgaacatag ggctcgggcg acattcagtc
     9181 gtcattcgaa cagacgacgg acgattacga tctcttgagc atgccacttt cttgatcaag
     9241 ccatttctct cgaacaggcc gcttatccac cgatcggagg atgcctcgtc acgacttgat
     9301 gatcgcggac agtaacgatc ggcgagtgag atagatcaca gcaccaccta cccatttcgc
     9361 gctacgcaat gcgagaggtg gggaaatacc gccttggaaa tgccattgaa tcgtaatgtt
     9421 agtccgacgg actgcgaccg ctatttcatt tccgcgttac tgcatttgag gctaagagcc
     9481 atgaacgata aagagcaatt ttcggcgctt attggcgata tctacgacgc cgcgctcgac
     9541 cccgcacaac gcatcgacgt cctcgaaaaa attgcgcgtt ttagcggcgg tcagtcggga
     9601 ggccttctct cgaagcattc tttgggaaaa tccgagaatc tgtattgtta tgtcggggcc
     9661 gatccggaca gcctccaggt ctattcagag agctatccga aactcgaccc gaccgctgat
     9721 ttgccgtact tcggcgtgca acaggtcgtg agtgctgcgc agttcgtgcc ccacgacgaa
     9781 tttcgccgcg gccgctttta tcgcgagtgg gcgcggccac atggctgggt cgacgtcgca
     9841 agcgtggtga tcgagaaatc ggtgaccagt tgcacgtttc tcagtgttgt tcggcatgag
     9901 gcgagcggcc tggtcgacaa cgaaatgcgc cgacgcatgg cgctgctggt accacacgtt
     9961 cgtcgcgcac tgctgatcgg caagaccatc aatctcaagc aagcccagag ggtctgcttt
    10021 tcggatattc ttgatgggct gagcgccggc atgatcctgg tcgacgtcaa cggccagatc
    10081 gtccacgcca acgccgccgg caatgccatt ctcgaggccg ccgacttcct gcgcacggtc
    10141 tgcggccggc tcgttgccgg cgatcccgcg atcaacacgg ccttgcgcga gatcctggtc
    10201 gccgcaaacg cgggcgatgc ggtacttggc gtcaagggca tagccctgcc gttgaccgcg
    10261 catgatggcg tgcgctacgt cgctcacgtc ttaccgctga cctcgggcgc acggcgcgtc
    10321 gcctgccttg cctacaacgc ggtcgccgca ttgttcattc gcaaggcagc cctggaggct
    10381 ttcgctcctc ccgaagttat tggtgagatg tacaagttga cgccgaccga gctgcgcgtg
    10441 ttgcttgcca ttgtcgatat cggcggtgtc cccgaggtgg cggcggcgct gggtgttgcg
    10501 gtcaccacca tcaagaccca cctcagtcgt ttgtttgaaa aaaccggcgt tgctcgtcag
    10561 gccgatctgg tcaagcttgt cgccggattt tctacgccac tggcaggctg acgcaatctc
    10621 cctggcgagg atcacaaccc ggtgccgctt cagacgccga gaatcaatac cggaattgat
    10681 gatttgcaat catcgcggaa tgatccgcag attgcagaaa tgtccgtcgg ttccgttgtc
    10741 cacatatagt ccgaccgtgc cgcgcgcttc agctccacgc ttcaaatcgg tcacaacgag
    10801 aacgggttgc gtggcgtcgt cgacaaagag atcggcccgg ctgccggaca gttcgacgcg
    10861 gatcctgatc cattgcgccg gcacgacatc cacataggac tcgtagcgag cgggcgcttc
    10921 acgacgtaac cggtcgaaat cgtagccggg ataagagaag tactgcgtgg catgattgcg
    10981 ccgaacctgg tcgtcagcgc ggccgttggt gggacgcaga tagataccct cgcaggcgaa
    11041 actgccgccc gcttcgtcga tccggaaagc cagccctgca aaaccgcgtg cgcctgccgg
    11101 cgcgctcggc agaggtgctc ccgcgagctc tacttcgatc gtgccgtcgt gcatgtcgag
    11161 gcccggcaca taagcgaagt agtcccgatc cggcccggga taggcgcccg caagccgtac
    11221 ttccacgcag cttcgcccat tgaaggacgc cggagcgacc gacacgttgc gtggcctgac
    11281 ctgggccgca tcggccagcg acaggaccaa cgggcgctgt tccggagccg cagcgcgcag
    11341 tgccttggta cacaggaccg ctgccggtcc cgccaagagc aagcgcctgt tcagcaacgc
    11401 caccggctga gtgttcgtcc gcgacgaacg ccgtaactgc gcgtctgttt tgtgaatcat
    11461 cgtgatcgtc cgtgatcgcg tttctgactc tccgtcaggc acagtttcgc atgtcacgcg
    11521 cttgccgcgc gtcgtccgat tgaacgaaga gcgattcaac agatcgtcat caagttttgt
    11581 ctgaacgtgt ggtgacgaat cgccagccaa aacctatcta cctgcccact ggaggcagag
    11641 ctcgatgcgc gcatctatca accaggtcgc ttcgcccgtt cgctatccgc gggctgaaat
    11701 gagtattcgg gaaccgagag taatgttgaa tcgtattttt ttgatcggag ccatggttgg
    11761 catcctgttg gcgcatgggg ttgtcttgta caaaattgat tcaggggtcc gcgccagtga
    11821 catcaagccg gttatggcct cacgatctca caaagccttc tggtgagcca accttcacat
    11881 tccactccaa gcggaagcca ttgaaatatc tgcggaattt acggcgattg cgacacgacg
    11941 tggcttcgga gagctgaacg cgcacctcct gcaccgggcc tgatttccac ccgccgtttg
    12001 gtgcatttcc ggtcggatca aacgcggcgg tcgtaccgcg gtgttgcgtc ttgatatacg
    12061 accaatatac gcttgggatg gacgtacccc aagcctgctc gctcgccgct tcgctggcgg
    12121 atgtgaggac cgatttgaaa tcgaaaacat cgataaaacg ccggtctcga gccgtcgagg
    12181 cagaggacgg cacgctgaca gatcgggcct atcgcgagct tgaagagatg attgtcacgc
    12241 tgcaattgtt gcctggaacc gtgctgtcgg agcaggctct tgcggtgcgt ctgaagatcg
    12301 gccgaacccc gattcgggaa gccctgcagc gcctcgcgag ggacgggctg gtggtgatca
    12361 tgccccggcg tggcatcatg gtctccgaga tcaatttgag gctgcaactg cggctgctcg
    12421 aagtgcggcg cgagctggaa cgtctgatgg cgagcctggc ggcggaacgc gccacagcgg
    12481 acgaacgccg cgaattcgcc gaggtggcag aagccatgct cgtcgccgcc gcaaagtctg
    12541 acgacatcgc attcatgcgt ctcgaccagc gtttcaacat cctgatcgcg accgcggcgc
    12601 gaaacgaatt cgcccggcgg tcgatgggac tgatgaacgc gttgtcgcgg cggttctggt
    12661 atcagcacta tcaggaagtc gcggatctgc cgcttgccgc caagctgcac gccgcggtcg
    12721 cggaggcggt gtcgcagaag aaaatcaagg ccgcggcgac cgcgtcggat cggctgatcg
    12781 actacatcga ggatttcgcg cgcaagacga tcgatacctg acccccaatc aatcgaatgc
    12841 aatgacgctg cgaatggttt tgccctgctt cagcgcggcg aagccgtcgt tgatttcgtc
    12901 aagcgtgatg cggcgcgaga tcaactcgtc aagcaacagg gagccgtcga gataggctcg
    12961 cgcgagcagc ggaaaatcgc ggcgcggccg cgcgttgccg taactgacgc ggcggatgcg
    13021 ctttttcctg catcagggaa ccccagcgaa acgagacatc ctggttcacg tcgatcttgc
    13081 cgagccagat gacctgcccg ccgggccgca cggcttcggg cgtggtccga aaagccgacg
    13141 cgctgccggc cgactcgatg acgacgtcga caccgcggcc gccggtctcg cgtcttcccg
    13201 cagcgatcac gtcgtctgcc gacgcgttca cgccatgcgt ggcgcccatc cgggcggcaa
    13261 gcgcgagttt cgccggatcg agatagacgg cgataatcgc tcccgcgcct gcgagccgcg
    13321 caccctgcac ggccgcgagg ccaaccgcgc cgcaaccgat caccatcacg gtatcgccat
    13381 gcgcgatcgc gccgagattg agcgcggcgc cgacaccggt catgacgccg cagccgatca
    13441 ggcaggcgcg gtcgaacggg acctctttcg gaatcggaat agcctgctga tccctgacga
    13501 tgcaatattc gccaaatgac cccaagaaca tcaactgctg aagctcgcgg ccgtcggcca
    13561 gcatggcgcg gctttcgcca tcgaacgccc gtccctgtgg tccgccgccg agatactcct
    13621 cgcacaggat cggcgaatcg cggtcgcaat aaaaacaatg cccgcaatgc ggattccacg
    13681 acagcacgac gtggtcgcca atcttgacgc cgcgcgcggc cgtaccggct tgttcgacga
    13741 cgcctgcagc ctcgtgaccg agcacaatcg gcaagggata gcgcagcgat ccctcgatca
    13801 cctccagatc ggtgtgacaa aggcccgccg cgcgaatccg caccagcacg tcggtcggct
    13861 tcaagctcgc ggcggtgacg gtttcgaccg acagcggcgt ttgcggtgca tgcagcaccg
    13921 cggcacgata ccggagcacc atttatgcga ccgccttgcc gtcaagcgct gtcagcgcca
    13981 ggtcgatatc atccgacgtc atttcccact cgttttcgaa attcgcaacg atggcgcgca
    14041 tgaaggtctc gaccgcggtc atcgcggcag cgttgtcgcc gagatgatcg accagcagcg
    14101 ccagtgcgag ctggcgcggc tcgggccctt catagctcca ctcgaaaccg ttcctggtga
    14161 actccatgac gtcaatgcga ggatcgagcg gtgccccatc aaccgtcacc ttgacgccgt
    14221 cgatcgtccg gtctcccaga tagtgcttca ttcggccctc ctgttgttaa atgtcagcac
    14281 gcgcggttgg tcacgaagcg gcttcgatct tgcccgcgat cacgcgcatg acgtcgcggt
    14341 cccgatacgc ctcttccaga gtgcctgcaa acatcacctc gccgcgttcc aatacgtata
    14401 tccgatcggc gtagtcggga atgtggtgaa cgttggattc ggcgatcaga acgcctctgc
    14461 ccatcgcgcg gatcgaagct acgccctttg cgatcattgg aatgatggcg ggcgacagac
    14521 cttcgaacgg ctcgtcgagc agcaataatt tcggatccag cgccaatgcc cgggcaatcg
    14581 acaccatctt gcgctcgccg ccggaaagct gcgcgccgcc gcgcgccaga tattgccgca
    14641 gtttcgggaa cacgctgtag gcttcttcga tgcggatgcc ggccgaacga ccagacggcc
    14701 gcgtccaggt cgatagctcg atattctcgg cgacggtcaa gtcggcatag acttctgact
    14761 cctcgggcga gaacccgata cctttctggg cgatctgcca ggtcggcagt ttggtcaggt
    14821 cggcgccctc gaaaatgatg tcgcctgata cggctttgcg ataacccatg atgctgcgaa
    14881 atgtcgtggt cttgccggcg ccgttgcgcc cgacaagaca gaccagttcg cccgccgcga
    14941 cgttcaggct gatgtcggtg aggatgcggc tgccctcgat atcaacgctt agattcgaca
    15001 aactcagcat catgacagct ttcttaaagt ctattcaaag ccttacctca aagcctcaac
    15061 tcaaagcctt acctcaaaag ccttgggcgt atccccacga cggcagcgat catcgccggg
    15121 tcggcgaaga agatatccgg cttcatatcg gcgagcagcg tgccgccttg cagcgcgacg
    15181 atacgatgcg agtaccgctc aacgaggtcc atatcgtgtt cgacctggac gatggcacgc
    15241 acaccggccg ccctggctgc ggtgacgagc acttccatga tcgcatgctt gtcgccgctc
    15301 gagacgccgc tggtcggctc gtccagcaga ataacggtgg gattgagcgc aaaggcgctg
    15361 gcgatatcga gcaatttctt ctcgccttgc gacagtgtcg aagccacgat gtcgagcttg
    15421 gcgcgcaggc ccagcacctc ggcgacgcgc tcgacgtcgg cttgaagcac ggcatcgcgt
    15481 gtgagcgagc gaaacggatt ggaagcgcgg cgcaaacgcg atgcgaccgc gaccgccagc
    15541 gtctcccgca ccgtaagagc gggaaagatg ttgaccagtt gaaatgcgcg cgacattcct
    15601 agtctagcga gttcgaccgg cccgatgccg gcgatatcgc ggcccaggaa gcgaacctct
    15661 ccaatggtcg gtcgcagcag accggtcacg acattgacca gcgtggtctt gccggctcca
    15721 ttggggccga cgatcgagac aaactcgcca tcgctgacct ggaaggagat gttttgaagc
    15781 gccttgaacg cgccaaagga tttcgagacg ttgacggtct cgaacagtgg cgccgcatca
    15841 gcggcgggtg cgtggttggg ctgcatcttc atcgccctcc gttgcgcctg tgagcgagaa
    15901 gttcgatcgt gcccgataga ccctgcggca tgaagaccac gagaagcacg agcacgccgc
    15961 ccatcacaaa acgccagtat tgcgtgatcg acatcacgaa atcctgcaat atgacaaaag
    16021 ccagcgcgcc aatggccgga ccggcaaacg ttccgctgcc gccaagcaca gccatgaaga
    16081 ccaaattgcc ggagtgggtc caatatgcca attcgggatc ggccaagccg gtggtgatcg
    16141 cgaggatcgt tccaccgata ccgccataga tcgccgagat cacgaaggcg aagagccgca
    16201 tgcgaaaaat ctcaaccccg acataagcgg ccttgccggc attttcgcgg atcgcgcgca
    16261 aatgcaggcc gaacggcgac cgggtgatcc gccacatcaa gagaccaagc agcgcaaaca
    16321 ggacaagcgc ataataatag aacgggccgg tcagaaacgc ggtcttgccg ccgcgccatt
    16381 ccatgccgag caagagcggc cgcagcaccc gcataccctg gtcaccgccg gtgatgctgt
    16441 agaacttgaa aagaaaggag tgaaacaaca tgccgaaggc cagcgtcaac atgccgaaga
    16501 agattttggt atagtggacg cacaagacgc cgatcaccag cgacaccaat ccggacacca
    16561 gcgcggccgt gatcagcaga acttcaaagc tcaatatgcc aaatttcgac gtcagcacgg
    16621 cggcgacata ggcgccgacg gcaagaaaca gcgcatgccc gaacgacagc agtccggtgg
    16681 tgccgaacag aagattgaaa cccaacagcg cgatgccgta gccgatgaac ggcagcagca
    16741 gcagcagcgc atagggcgag gcgaccgccg gcagcaacgc catcacgacg atcagcccaa
    16801 gcaggaccca caatccattg agccgcggta ccgtccgtcg cacggtcgca ggggaagcga
    16861 tgtcgatcga tggatgggtc acgcggtcgc cttcccgaac aggccggccg gtctcaaaat
    16921 gagcaccacg acgaccacca gatacaccga cagcatctcc atttcgggca tcagcgaaat
    16981 cgccgccgca cgcatcaagc cgacgacgag cgcgccgacc aaggcaccgc gcatgctgcc
    17041 aaggccgcca atcaccacca ccgcgaatga ttcgaccacg agctccaccg ccatatcgag
    17101 cgacgccgcg cccgatggca ccaccagcgc gccgcctacc gtgcccagca tgcagccgac
    17161 ggtgaagaca agcgcgaaga ttttcgaagc gttgacgccg agcccttccg ccatgtcgcg
    17221 gttttccgcg gtggcgcgca cgatacgccc ggtcttggtg cgctcgagaa agaggcccaa
    17281 cgccgccgcg atggcaagac ttgccgcgat caccagcaga ttataggtcg gcacccgcac
    17341 tccccaaata tgcgcggtgc cgcaggcgag ataaacgttg tccatggtgc gcggcgtagc
    17401 accccataga tagcgaaacg catcctgaaa catcaggaga aagccgaagg tgatcagcag
    17461 ttgatagctc tcatcgcggc gatagaccgt gcgcaagacg cgctcgatcg gcagtccgac
    17521 gaggccgatc gccaatccgg cgatgatgag ggcaggaaaa aacaacaaag gcggcatgcc
    17581 gagcttgatc gcgatgccga ccgcggtgat tccgaaatat gcgcccagcg cgtagaacga
    17641 cccgcaggcc agattgacga tcttctgcac gccgaacacg agctgcagcc cggcggccac
    17701 cagaaacagc accgcggcgt ggaaggtccc gccgaggacg acatccacaa attcagtcat
    17761 cctcagaccg gcatcttcgc gctcttgatc cactcccaga aatccgcgcc gggcggcttc
    17821 tgcaactgct cggcctggaa ggtgtcgatc gaagccagcg tcggaaaatc gtatttattg
    17881 gtgttggtgg acgggccctg atagaaaacc tgttcggcaa tcttgtcctt gcggaagcgg
    17941 cccttgccac cgaggctttc gacctccagg ccgggcatcg cctgtgccac ctgctccggc
    18001 gtcggccatt tgcccgcggc cttttgcgcg gcctcgacgc cggccttgta ggtggcgagc
    18061 gcgaaatacg ccctgtccgc ctcccaatgc ggtgcttcct tgtagcggtc catgtagtcc
    18121 gcaacgaatg ccttttgcag cgcagaggcc tgcggatgat cgaaatacag cgtgttgtgt
    18181 ccgaagatca cgccttccgg catgaattcc ttcttgagct ggttgatctg ccagccgacc
    18241 gccggcaaca ccatcttgac accgtcgaac agaccggccg catgaccctg cttcatgaac
    18301 accggcaaat cggcaaacag catcgaggag aagatcagat cgggcttggc cgcattaagg
    18361 gcggcgatat gcgaggtcag gtccatcgtg ccgaccttcg gccattgctc ggcgacgaat
    18421 tccgcctcaa tgccgtagcg cgccaggatc tgtctaaaag cttcccaatt gttgcggcca
    18481 taggaatagt cgggattgat gccggcgacg cgcttgaatt tgcctttgaa atacttgatc
    18541 gccagcagcg atcccatcac cgcttcgcat tcgttatcgg tcgagcggaa cacgtagcgc
    18601 gaattcggca tcgtctcctt gacgccatcc tgggtggtgc cgtcccacat caccagcagc
    18661 gcctgctcct cttccgccac gggcgcgacg ccgaggctga cgccggtcga gatcaatcca
    18721 tggaccgcct cgaccttttc ctgcaggatc aagcgccgaa agcgctcgat ggtgtccttt
    18781 gggttggtct cttcttcgaa caccagctcg atttggcgcc cggcgatgcc gccgtccctg
    18841 ttcatccgct cggccgccca ttgcaccgcg cgaatgccac actcgcccgc ggtgccggct
    18901 gcaccggatc gcggcgcaat gattccaatc cgcagcggct tatcctgcgc caaggcgggc
    18961 gcgaatagtc ccggcgcggc agctgccagg acaccggtgc ctgcaagtct ggaaaaatcg
    19021 cggcgcgtga ctttcatcgg tgcctcccgt tatcagttgc gcgtctatcc gcgcgctcgt
    19081 tgccatacca taaatatatc agcgacatcg catcaagcgc ttttttcagg ctaggcctgt
    19141 caacaacccg aaaaacccgt tgccgaagta atagaccggt gatatatcag cgacaaaatg
    19201 gctcgcaaat ggacggaggc ggcggatgga gatatcatcg agtccaatga cggaacggca
    19261 gcggaactac cgcgcgacct accgccaccg catcgccggc tggtacaatg gctggctcca
    19321 tgctttcgtc atttatgtga tcggattcac cgccctgtcg gtttatttcg ctgacatcag
    19381 cgacttgcgc tggtgggaac tcgggatcgt gcccgtaacc tttctcgccg ccaatttctt
    19441 cgaatggtgg attcaccgtt tcgtcatgca ccgtccgtcg caggtgaaag cgttccgcgc
    19501 catctacaac cggcacacgc tgatgcatca tcagttcttc acggagcagg agatgcgctt
    19561 cgccgatcac aacgactggc gcgtgacgtt ctttccgccc tacgcgctgg tgaccttcac
    19621 gatgatgtcg atcccgccgg cgcttgttct cggctggctg atctcgccca acgtcggctg
    19681 gctgctgatc tcgacgacga catcgatgta cctgatctac gaattcatgc acttctgttg
    19741 ccacgtcgac gaaaactggt tcgtccgcca tgcgcccttc gtcaacacga tccggcgcca
    19801 tcacaccgcc catcacaatt agtccatcat gatggagcgg aacatgaacc tgacctttcc
    19861 cgtgatggat tggctgttcg gcacctccga tctggatcgc ggcctggttg gccatctcct
    19921 gaacggctac gacacccgct tcgtgaaaac caatatgcgc aagacttcgc ggacgccgaa
    19981 accgaacgtg acgggacacc cgacggggtt agctaccccg gccgaatgag cgggggattc
    20041 gtcaagcaac cggcagacaa gccatggacg ccatcgaacc gcattcgatt tccctacttt
    20101 ggttcacggc gttcgcgacg gtctgcgctg tggctttcct cgtcgttgcc ggcatgtttc
    20161 cgctgcgctc gcgtccggac tcactgaagt cgggcgtggc gacgttgctg gttgtaagca
    20221 acgtcgtttt gctggtcgtc cttttgacgg gtacgggctt gtacggctac gccgagctga
    20281 ggcggagcac gctcgttgtc gtggccggcc ttgttgtgct gttcgcgccc gggcttttcg
    20341 aagtctggcc atcgtcactg cgcgatggaa gcgccggact gaccgcattg gcaggaatcc
    20401 agatgctggc tcttgcagct ctggccaagc tcggcggcct ggcgtgggcg agcatgtcgt
    20461 gatcggttgg gaggatagac aatgccggta aagatcaagc ttggcctgtc actgcttgtc
    20521 gtgctggtgg cggcgattgc ctcctattat caacacgcgc tcgggcacga tgttgtccag
    20581 tacgtggttg ccttcctcgg attcttcatg attttcgcga tgtggctgtt tcccgaagtc
    20641 aagcgtgagg aatccgctgg tggagttcgc agagacaccc tgccccggta gcaacctctg
    20701 tgttcgatca agcccgtgca gatctatacg ggcggggtgg ctcagcgcta agagggccct
    20761 cgcccccgta gcgtcagtgg ctgatcatcc acgggcgcgc ggtcggaaaa ctcttcgcgc
    20821 cgcttttggt ctgcttgacg agccgcgacg gcttggtcga gcagcagccg caacccgggc
    20881 catgcgacgc cttgtattcg ccgacggttt tcggcgcatt agcactgcgc tcgttggtgg
    20941 cgttggcctt gcgcttgtcc gacggcatgc agaagaaatt cggcgccgtc aggatcacgc
    21001 gcggggcttc gagttcgcat cgcggacagt cctgcggcgc gtcgcattcc gccatcggcc
    21061 gcatgtcggt aaacgggccg caatcgttgc agagatattc gtagaccggc attttaatcc
    21121 tcccgcgaga tccaattcac accattgttt tgacgcgttt tcttcacgcg aaccggtgcc
    21181 catcccggat caagtccggg gcaggctttc gctcgaaaac gctatcattc accacggcgg
    21241 atgcggggcg gcaaatactt aatagtcccg catcccgtcg cgcggggagc tacttgtcct
    21301 gcgatagcgg catctggatg tcgcccttga tgtgtttgat cgggccggcc gacgacggca
    21361 tgatgtcgaa gtcgaagatc tccgtcggca gccacagcgt cgcgcaggcg ttgggcacat
    21421 cgaccacccc ggagatatgg ccctggcacg gcgccgtgcc caggatagaa taggcctgcg
    21481 cgccggaata accgaacttc ttgaggtact cgatcgcgtt caggcaggcc tgccggtatg
    21541 cgatgtgtac gtcgaggtaa tgctgcttgc cctgctcgtc gaccgagatg ccctcgaaga
    21601 tcagatagtc cttgtagttc ggcgtgaccg gcgacggctt gaatacggga ttcttgatgc
    21661 cgtatttgtt gacgccatcc ttgatgatgt cgaccttgag atgcagccag cccgccattt
    21721 cgatcgcgcc gcagaaggtg atctcgccgt cgccctggct gaaatgcaga tcgcccatcg
    21781 agagaccgcc gcccggcaca tagaccggga aatagacctt tgagccccgc gatagatcct
    21841 tgatatcgca attgccgcca tgctcgcgcg gcggcaccgt gcgcgcgcct tccgccgcgg
    21901 ctttcgcttt ggcttcgcct ttcagccggc ccatatgcgc ggtcgggccg aacggcggat
    21961 tggccaaacc aggcacgcgg gtcggattgg tcgcgatcag cgcggtctcg cgctcgttcc
    22021 atgtcgccag caattttgga tccggcagac agccgatcag accgggatga atgagtccgg
    22081 caaaattcac gcccggaacg tgccgggacg aggtgtacat gccatggaag tcccagatcg
    22141 acttctgcgc cagcgggaaa tggtcggtca ggaatccgcc gccgttctgc ttggagaaga
    22201 agccgttaaa accccacagg ctttccttca tcggaccgac gtcgagcaga tcgacaacaa
    22261 ggagatcgcc gggctccgcg cccttgacgc cgatcggacc ggacaaaaag tgcacgatcg
    22321 acagatcgat atcgcgcacg tcgtcggcgg aatcgttgtt cttgatgaag ccgccggtcc
    22381 agtcgtaggt ctcgacgatg aagtcgtcgc ccggattgac ccacgccacc atcggaacgt
    22441 cgggatgcca gcgattgtgg atcatatcgt tttcgtaggc cgattgcgtg agatcgacct
    22501 tgatcagtgt ctctggcatc aaaagctccc ctgtttacta gtgttggtta cgatctagac
    22561 ggacaaatat ttcgaaatct gcgcggcatc gacgccctcg cgcgcatcgt cgcgcacgat
    22621 ctcgccgttc tcgatgacga gcacgcgatc ggcgatgtcg agcgcgaagc tcaggacctg
    22681 ctcggaaacc acgatcgaca gtccgcgctc gtcgcgaatg cgctttaacg tccgcgccat
    22741 ctcccgaatg atcgacggct ggatgccttc ggtcggctca tcgagcaaaa gcaccttggg
    22801 cttggtggcg agcgcccgcg caatcgcaag ctgctgttgc tggccgccgg acagattgcc
    22861 gccgcggcgg ctcttcattt ccagcagtac cggaaacagc tcatagatgt cgccgggcac
    22921 ctcggtctcg cccgacgcga ccaggccggt ctcgatgttt tccttcaccg tcatggcgga
    22981 aaaaatcatc cggccctgcg gcacataggc cagccccttc gccacacgct cgtagctctt
    23041 gagcgtggag agctcggcgc cttccatggt caccgagccg ctcttggtcg gcacgattcc
    23101 catcagcgac ttcatcagtg tggtcttgcc catgccgttg cggcccatga tcgcaatgat
    23161 ctcgttgggc gcgaccgaga cgttgaggcc gtgcagcact tcgctctggc cgtaagcgac
    23221 atgcagatcc gagatagcca gcattaaagc gctccttgtc tttcgagcat gatctagtcc
    23281 ggaaaccgga ccgcactttt cgggatcatg ccctagtgcc cgagatagac ttcgatgact
    23341 ttcggatcgt tcttgacgtg ctccatcgaa ccttccgaca ggatctggcc ctgatgcaga
    23401 accgtgacct tgtgggcgat gtcctcgaca aatttcatgt cgtgctcgat caccaacacc
    23461 gagcggtcct tgatgatgcg gttgagcaat tcggcggtct tgacgcgctc gctgacgctc
    23521 atgccggcca ccggctcatc gagcatcagc agttcgggat cctggatcag cagcatgccg
    23581 atctcgagcc attgcttctg gccgtggctg agcagttcgg ccttcacgtt gagccggtct
    23641 ttcaggaaga tcatttcggc gacttcatga acccgatcgc gcacggcggc gtcacgggtg
    23701 aaggtcagcg caccgaacac ggtacggccg cgcggaaacg aaatctcgag attctcgaac
    23761 accgtgagat cgtcgtagat cgacggcgtc tggaacttgc gccccacccc cgcctggacg
    23821 atctggttct ccttcatttt cgtgaggtct ttgtcgcgaa agtggatcga cccggatgtc
    23881 gctttggtct tgccgcaaat cagatcgagc accgtggtct tgccggcgcc attggggccg
    23941 atgatgacgc ggatttcgtt ttcatcgacg tagaacgaca ggtcgttgac tgccttgaag
    24001 ccgtcgaacg aaaccgtgag cgcctcgacc gagagcagaa acgccttggg ctggtggcct
    24061 atgagcatga tctatctcct cattccgccg gtgcgccgtc agcaaccgac ttgtcggtcc
    24121 agccgtcgtc ggatttcgat ttgcgcgacg agatcagccg atcgaggtgc ggctgcacat
    24181 agtcgctcca gatgccggcc agaccattcg ggaaggcgag aacaaccgcg atgaacaatc
    24241 cgcctaaccc gaacagccac aattcgggaa aggattccga caggctggtc ttggcgaagt
    24301 tgaccaggag cgtgccgtag accgcgccca ggatcgagag ccggccgccc accgccgtat
    24361 agatcaccat ctcgatcgac ggcacgatgc cgacaaagga gggtgacatg aagccgacct
    24421 cgagcgcgaa catggcgccg ccgatcgcgg cgaatatcgc agccacgcag aaggcgaaga
    24481 tcttgaaatt cgcgacgcta taaccggaga aacgaacccg atcctccttg tcgcgcatcg
    24541 cgaccaggat gcgaccgagc ttcgagtgcc ggatgaactg ggcgatgaag atgcaaacga
    24601 acaggcagcc gacctcgaag aagtagagaa cgatcttggc gtgatccggc cggatgtccc
    24661 atcccttcag ggtgcgcaga tcggtcatgc cgttgatgcc gccggtgtaa ccctgctgcc
    24721 ccacgatcag gatggtcagg atcgccgcga ccgcctgggt gatgatggca aagtaggtgc
    24781 caccgacgcg gcgtttgaac atggcggcgc caatgatgag cgcaaagata cccggcacca
    24841 ggatgacggc ggcgatggtg aaggtcaggc tgtgaaaggg cttccagaac agcggcagcg
    24901 acgtgatctg attccagtcc atgaaatcgg gaatcccggg ggtcgactgg atcttggtat
    24961 tttccgggct cgatgcctca agcttgagaa acatcgccat gcagtagccg cctagtccga
    25021 agaacacgcc ctgccccagg ctgagaatgc cgccgtatcc ccagcagatc accagaccca
    25081 gtgccacgaa ggcataggtg aggtattttg cgaccagatt gagccggaag acatcgagac
    25141 agagcggcag gatcaccacc aggaaaacgg cgagcaccag gatcccgata agctccgatc
    25201 gattgacaaa gcgattgtcg gtcatggctt cagccctttt tgttatttgc ggaccttgag
    25261 ggcgaacaga ccctgcggcc tcagcatgag aattccgaca acggcaagca gcgtcaaaac
    25321 cttggccatt gaccccgaca tgaagaattc gagcgtcgac tgcgtctgcg agatgctgaa
    25381 ggccgatgcg atcgtaccga tcaggctcgc ggcgccgcca aacacgacca ccaggaaggt
    25441 atcgacgatg tagagctggc cggatgtcgg cccggtcgag ccgatcatgg taaaggcact
    25501 tcccgcgacg ccggcgatgc cgcagccgag cccaaaggtg tagcgatcga ccttttcggt
    25561 gttgatgccg accgcgccgg ccatgacgcg gttttgcacg acggcgcgga cctggcggcc
    25621 ccagcgcgac ttgtacatga tgtaggcgac acagagcgtg atcaggacgg tcaggcacat
    25681 cacgaagacg ccgttgatcg gcacctcgat gctgtcggaa acatgcagcg aaccaagcat
    25741 ccattgcggc agttcgacgc cgacttcacg cgcgccgaaa atcgagcgat aggcctgctg
    25801 caggatcaga ctcaagcccc aggtggcgag cagcgtgtcc agcgggcgct tgtagagatg
    25861 ccggatcagc acccattcca ccagcatgcc gagtgcgccg gacgccagaa aagccaaaat
    25921 catcgccagg aagaaatagc cggcaaacaa acccggcagg tatgactgga agagattcga
    25981 cgtcatccag gtgacgtaag cgccaaggat catgaactcg ccgtgcgcca tgttgatgac
    26041 ccccatctgg ccgaagatga tggcaaggcc gagcgccatc agcacgtaga cggaaaacag
    26101 gatcaggccg gcaaagccct gcatgacgaa gatggagccg agatcaccaa tcgaatagtc
    26161 gccgaacatc gattttctcc gtctgaggga agtcccgcgt cgcgagcggg ttcgcgacgc
    26221 gggggtcttg agcgtggatc gtcgatccac gccagggagc ggttgcttta gcggaaaacg
    26281 cgacagacgc cgcgcgccta ctggtaaccc ttgttactgg tagcccttgg ggaacggatc
    26341 cggttcgacc agatcggctg tttcatagat cagctcgaac tggccatcga gcttggcgcg
    26401 cccgacccgg gtcttcgacc agaggtgatg gttttcatga atgcgcacgt aaccttccgg
    26461 cgcgcccttg aattcgatac ccggcgaagc cgccgcgatc ttgtcgatat cgaagcttcc
    26521 ggccttttca gccgttaact tccacagcca cgggccgaga taggccgcct gcgtcacgtc
    26581 gccgatcacg gtcttctcgc cccacatctt cttgaacgcc ggcacgaagg ccttgttgtt
    26641 gggattgtcg agcgactgga agtacttcat gcaggcatag gcgcccgc
//
LOCUS       DASWGM010000203         8946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1537462,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000203 DASWGM010000000
VERSION     DASWGM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537462"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VGA15_06265"
     CDS             <1..501
                     /locus_tag="VGA15_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587334.1"
                     /translation="QSGGTTWTRIMFERQVLGEDYWAKQAKTHPILYPSGAPMADSLV
                     RGEIAMGPLLYNAIYPKQKDGAPIKIFFPPEGAPVNPYASGIPKTAAHPNAAKLFLNW
                     CLSKEGQTFMIKELGNLTSLKEPPIYPEGFDPKIVKVWLPKFDDYVKLHAAWVADWDK
                     TFGYRQ"
     gene            530..1633
                     /locus_tag="VGA15_06270"
     CDS             530..1633
                     /locus_tag="VGA15_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587335.1"
                     /translation="MTATLDVTDLRKQFAIGRPAIDGVSFAVPAGEIVVLLGPSGCGK
                     TTTLRCVAGLEHPTSGEVSIGGRVVSSPERGILVPPRSRDLGMVFQSYAVWPHMTVRQ
                     NVIYPLKHRKIARAEAARKVDEVLELVGLSEYADRPVVALSGGQMQRVALARSIVYRP
                     QLLLLDEPLSNLDAKLRLRLRDDLRVILKQTGMTALYVTHDQAEAVVLGDRIGVMRDG
                     KLLQMGSPDEIYNRPADLFVANFTGATNEMAGTLIECHGMSGLADVGCGQRVEVALLH
                     AISAGEKIRIALRPENIALGKQGGSNAFNARVLDRRYQGTQTVYDIDLFGHRLEVLEL
                     GTAARHQVGAETSVCLPREGCWAYRDTGSSSYD"
     gene            1687..2775
                     /locus_tag="VGA15_06275"
     CDS             1687..2775
                     /locus_tag="VGA15_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX9587336.1"
                     /translation="MRIVVLPGDGIGPEIAAATLNVLHAASERFQLNVQLEQHAVGHE
                     SLELSGATVRPGLLETVRSADGLILGPTATFDFKDEHSGEINPSKYFRKNLDLFANVR
                     PAKTYPGMRNRFGAFDLVVVRENTEGFYADRNMEQGNSELLVTPDVAISLRRITRHCC
                     ERIAQAACKLAMRRRKHLTIVHKANVLKIGDGMFLDICREAARACPGLAVDDVIVDAM
                     MAHVVREPDRFDVIVATNMFGDILSDLTAELSGSLGLGGSLNVGAEYAMAQAAHGSAP
                     DIAGRDVANPFSLILSTALLLGWHGERRGEARFEEAGRAIENAVALAMQGGRATRDVG
                     GDLGTAATGQAIVDILQSDAATASAAAH"
     gene            3002..3391
                     /locus_tag="VGA15_06280"
     CDS             3002..3391
                     /locus_tag="VGA15_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9587337.1"
                     /translation="MNLKQTRLVTDDVQKLASFYEKLTGATAEVISRGYVEFQQSPCS
                     GLAITSAAAAEVYGGGVLRSAANRSLVLDFEVRDVDAHYTRLKESVSEWVQPPTDQPW
                     GNRAMLLRDSDGNLVNMFAVKRTPVMT"
     gene            3422..3850
                     /locus_tag="VGA15_06285"
     CDS             3422..3850
                     /locus_tag="VGA15_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587338.1"
                     /translation="MTNFWKLAPWLSRLIILAVAVLFMMISLKFVFDPQQAAANSGIV
                     IQSSVGYTNTRAGFGGFPLGFAAILVFCLFSSDRLLPALSSIVTVAAVILTVRLYGAV
                     QDATFGQSAHLLVPEAVLLVISLLGVVMEMRRRHRDAAKG"
     gene            complement(3915..4787)
                     /gene="sigJ"
                     /locus_tag="VGA15_06290"
     CDS             complement(3915..4787)
                     /gene="sigJ"
                     /locus_tag="VGA15_06290"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEX9587339.1"
                     /translation="MTLDRAFAELRPAMFALAYRITGNRADAEEIVQDAFVRLHDAKP
                     KDALRSLKAYLATITARLSLNRLRDQHARRETYVGEWLPEPLLTEGEPGVRAEDVSFA
                     LLVVLERLSPAERVVFVLRNAFDLSFEEIAPVVQRDAVACRKIFSRARAHMIEQRPRF
                     AVDRERHRALLRSFADAARGGDTARLVSLLDENVVLHGDGGGKALALKKPVVGGIAVA
                     HFLIAVTRTLPADASAEVVELNGSPGLVLKAGGRPVVAIVIETDGERIHTIFGVSNPD
                     KLDAIASAPVRSPA"
     gene            complement(4784..6046)
                     /locus_tag="VGA15_06295"
     CDS             complement(4784..6046)
                     /locus_tag="VGA15_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9587340.1"
                     /translation="MDATETTGHFPAYDIVILGAGYAGLMAALRLTQKKQELRIALVN
                     ASDQFLERVRLQESIVAPVAPRIPSIWAFLEGTGIEFFCGEVASLDADRRCVRITSGA
                     QQREIMFDEVIYALGSRVDADAVPGAAEHAYRLEAAEGPRSTSALRSRLQDNASRPMR
                     VITVGGAETAIEVAGEIKTAWPSVEMTMISSSRCGDFKGARVERAVRTELGRLGVRMI
                     DSETVAEVHLDEIVTDKGRRVPFDICVWSGGLRSAPVASGAGVATDPRGRIWVDANLR
                     SISHPHIIAVGDAAHPVAPTGAPYRLSAFVAITSGAYAADAILAQRAKRKLAPFSFST
                     FGQGIAIGRGGVGFPSYPDDKQAWFILTGRTARIVRNFFVWFISYALKIERRVPGFFF
                     WPGRRRVSWHQANEAIQRVRTAQKVQTA"
     gene            complement(6137..7015)
                     /locus_tag="VGA15_06300"
     CDS             complement(6137..7015)
                     /locus_tag="VGA15_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9587341.1"
                     /translation="MATAERSSFAKAAVYLGISRSALSETIRGLEEKLGVRLLNRTTR
                     SVALTEVGERLLAELRPALDSFEAAVESVNVFRDKPGGQLRLTVPRPAAKTVIEPILS
                     KFLAAYPAISIEIITDSGLTDIVRDRFDAGIRPGHRVGRDMIAVRVGEDARPTIVASP
                     DYLRRHPAPKIPGDLQSHNCIRMRFASGAMQPWAFEKRGKSMEVLVKGSLIVSDGDLA
                     LRAALDGIGIARLPLSRIDAHVGKKRLVPLLEDWAPRSVGFYLYYPSRRQVPAALKAF
                     VDFIKAHSRADGALRS"
     gene            6984..7796
                     /locus_tag="VGA15_06305"
     CDS             6984..7796
                     /locus_tag="VGA15_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX9587342.1"
                     /translation="MAKLERSAVATKAFISANPNFRIDRNLRLSHADNARLSNKHQSF
                     ISPADQNVARYDFQLWRSSGFCRAFDGEPVMTVVSRYHTLLVALHWILAVLIIAALAL
                     GALVMVKIPNSDPMKLEALRSHMIGGSLIAVLMLVRLVVRMRTEHPATPSAGNPVLDR
                     LAWASHRLLYVAVLGQAASGVMMALQTGLPGIVFGGGALPADFWVYPIRTVHYLFSRT
                     LMALIALHVAGALYHTLVLRDGLLRRMFFGRRIVAAADSRTALTKPIPGTRP"
     gene            7793..8344
                     /locus_tag="VGA15_06310"
     CDS             7793..8344
                     /locus_tag="VGA15_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587343.1"
                     /translation="MNYLTPAQASAQFMSLIVLAAVARWHIIPWLNRRARADALIALL
                     WVHVFRYVALQIFSAQHDGFPISDGGAMEIVVGDVAGAVIAFIAITLLRRRARLGILL
                     AWLLAAETAYDTVANIHGGVQEHLMGAASGVTWMILTFFVPMVVVSGGLMIWQLYARR
                     GEELGSSQERGVLPSDAPLRRAF"
     gene            complement(8374..>8946)
                     /locus_tag="VGA15_06315"
     CDS             complement(8374..>8946)
                     /locus_tag="VGA15_06315"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9587344.1"
                     /translation="VKDMKEFVAYAKANKDKMQFGSAGTGSAIHLGCALMNMVAGLDI
                     VHVPYRGANPAMQDLMSGRVDYLCDIITTAKPQIDGGAVKAIAILAKQRSPVLPNVPT
                     AVEQGFDVNAYTWNAFFLPKATPDAIVQKLNHATIEAMKTPAVREKLESAGLKFVSDD
                     RTTPEYLAKFVQSEIAKWAVPIKASGVSVD"
BASE COUNT         1634 a         2793 c         2808 g         1711 t
ORIGIN      
        1 cagtccggcg gcaccacctg gacccgcatc atgttcgagc gtcaggtgct cggcgaggat
       61 tactgggcaa agcaggccaa gacccacccc atcctctatc cgtcgggcgc gccgatggcg
      121 gactctttgg tgcgcggtga gatcgcgatg gggcccttgc tctacaacgc gatctacccg
      181 aaacagaagg acggcgcgcc gatcaagatt ttcttcccgc ccgaaggcgc gcccgtcaat
      241 ccctatgcct cgggaattcc gaagaccgcg gcgcatccca atgccgcaaa gctgttcctc
      301 aactggtgcc tgtcgaaaga gggtcagact ttcatgatca aggagctcgg caacctcacg
      361 tcgctgaagg agccgccgat ctatccggaa ggtttcgatc ccaaaatcgt aaaagtctgg
      421 ctgcccaagt tcgacgacta tgtgaagctg cacgcggcct gggttgcgga ctgggacaaa
      481 accttcggct accggcagtg atcgtcgcac gcatgaagct tttcaaatca tgacggcgac
      541 gctcgacgtc accgacttgc gcaagcagtt tgcgatcggc cggcccgcca tcgacggcgt
      601 cagctttgcc gtgccggccg gcgagatcgt ggtgctgctc ggtccgtccg gctgcggcaa
      661 gaccacgacc ctgcgctgcg tggccgggct cgagcatccg acatcgggcg aagtcagcat
      721 tggcgggcgc gtggtatcgt cgcccgagcg cggcattctg gtgccgccgc gctcgcgcga
      781 cctcggcatg gtgtttcagt cctatgcggt gtggccgcat atgacggtgc ggcagaacgt
      841 gatctatccg ttaaaacacc gcaaaatcgc gcgcgccgag gccgcccgca aagtcgacga
      901 agtgctggaa ctggtcggcc tgtcggaata tgccgaccgc ccggtggtgg cgttgtcggg
      961 cggtcagatg cagcgcgtcg cgctggcgcg cagcatcgtc taccggccgc aactgctgtt
     1021 gctcgacgaa cccctgtcca atctcgatgc caaattacgc ctgcggctgc gcgacgacct
     1081 gcgcgtcatc ctcaagcaga ccggcatgac cgcgctttac gttacccacg accaggccga
     1141 ggcggtcgtg ctcggcgacc gcatcggcgt gatgcgcgac ggcaagctgc tgcagatggg
     1201 atcgccggac gagatctaca accggccggc cgatctgttc gtcgccaatt tcaccggagc
     1261 caccaatgag atggcaggca ccttgatcga gtgccacggc atgtccggac tggccgacgt
     1321 cggctgcggc cagcgcgtcg aggtggcgct gctgcacgcg atatcggccg gagaaaaaat
     1381 ccgcatcgcc ttgcggccgg aaaacatcgc gctcggcaaa cagggcggca gcaatgcctt
     1441 caacgcccgc gtcctcgatc gccgctatca gggcacccag acggtttacg atatcgatct
     1501 gttcggccac cgactggaag tcctcgaact cggcaccgcg gcccgccatc aggtcggggc
     1561 cgagacgtcg gtttgccttc cgcgggaagg gtgctgggcg tatcgtgata cgggttcatc
     1621 gtcctacgat tagcggcagc agcgggcccc aagcgagcca tcaagcgagg catcaaggga
     1681 gttatcatgc gtatcgtcgt tctgccgggt gacggcatcg gcccggagat cgctgccgcg
     1741 acattgaacg tgctgcatgc agcgtccgag cgttttcagc tcaatgttca gctggagcaa
     1801 cacgcggtcg gccacgaaag cctggagcta tcaggtgcga cggtgcggcc cggtctgctg
     1861 gaaaccgtgc gatccgccga cggattgatc ctcggaccca ccgcgacctt cgatttcaag
     1921 gatgaacaca gcggcgagat caacccgtca aagtacttcc ggaagaatct ggatctgttc
     1981 gccaatgtcc ggccggccaa aacctatccc ggcatgcgca accggttcgg tgcgtttgac
     2041 ctggtggtgg tgcgggagaa taccgaaggt ttttacgccg accgcaacat ggaacagggc
     2101 aacagcgaac tcctggtcac gccggatgtc gcgatctcgc tccgccgcat cacgcgacac
     2161 tgctgcgaga gaattgcaca ggcggcgtgc aagctggcga tgcgccggcg caagcacctc
     2221 accatcgttc acaaggccaa tgtcctgaag atcggtgacg gcatgttcct cgacatctgc
     2281 cgggaggcgg cccgcgcctg tcccggcctc gccgtggatg acgtgattgt cgacgccatg
     2341 atggcgcatg tcgtgcgcga gcctgaccgg ttcgacgtca tcgtcgcaac caatatgttc
     2401 ggcgacattt tatcggacct gacggcggag ctgtcgggga gtctcggcct cggcggctcg
     2461 ctcaatgtcg gcgcggaata tgccatggcg caggccgcgc acggttccgc gcccgatatt
     2521 gccgggcgtg acgtcgccaa tccattctcg ttgatcctgt cgactgcatt gctgctcggc
     2581 tggcatggcg agcgccgggg tgaagcgagg ttcgaggagg cgggtcgagc catcgagaac
     2641 gcggttgccc tcgccatgca gggcggccgt gcgaccaggg atgtcggcgg cgatctcgga
     2701 accgctgcga ccggccaggc cattgtggac attctgcaat cggatgcggc aaccgcgtcg
     2761 gcggcggcgc actaacttcc tgagccattc ggcgcgacat attggcctga tattgacccg
     2821 cttttgattg gtcggatttt ccgaccgcgt catgcggata aggctggatt gtcgaacagc
     2881 gaccttggtc ctaattccgg accaaccgaa agccgttctg cctcgctcgc acgaatacgg
     2941 gcccagcgag atcgaatggc ggcggccggt acgttccaag gcatgcaaag gacaaagaac
     3001 catgaatctc aaacaaacca gactggtcac ggacgacgtc cagaagcttg caagcttcta
     3061 cgagaagctc accggcgcga ccgcggaggt tatcagccgc ggctatgttg agttccagca
     3121 gagtccctgt tcgggtctgg ccatcaccag cgcggcggcc gcggaagttt acggaggcgg
     3181 cgttcttagg tccgccgcca atcggtcgct ggttctcgac ttcgaagtca gggatgtcga
     3241 tgcccactac acgcgtctca aggagagcgt cagtgagtgg gttcagccgc cgaccgacca
     3301 accctggggc aatcgcgcga tgctgcttcg ggactcggac ggcaatctcg tcaatatgtt
     3361 cgccgtgaag cgcactcccg tgatgacgtg acgaacaacc gcattgctcc cggagcgccg
     3421 catgacaaac ttctggaagc ttgcgccctg gttaagccgc ctgattatcc tcgcagtcgc
     3481 agtgctcttt atgatgatca gtctgaagtt tgtgttcgat ccgcagcagg ccgcggcaaa
     3541 ctcgggcatc gtcatccaat ccagtgttgg ctataccaac acccgcgccg gctttggggg
     3601 ctttcccctg ggctttgcgg cgatcctcgt gttctgtctg ttctcgtcgg acaggctgct
     3661 gccggcgtta tcgtccatcg tgaccgtcgc cgccgtgatc ttgacagtga gactctatgg
     3721 cgcggtacag gacgctacct tcggccaaag cgcgcatctc ctcgttcctg aagcggtact
     3781 cctcgtcatc tcgctgctcg gcgtggtgat ggagatgaga cgacgccatc gcgacgcggc
     3841 aaaaggctga ccaaggccga ggccgtgacc gctccgccga cgagcgcgta aagtcaacgg
     3901 acggatgtca ggcctcaggc cggtgagcgc acaggtgcgc tcgcaatcgc gtcgagcttg
     3961 tccgggtttg agaccccgaa gatcgtgtga atgcgctcgc cgtcggtttc aatgacgatg
     4021 gcgaccacgg ggcgcccgcc cgccttcagt accagcccgg gcgagccgtt gagttcgacc
     4081 acctccgcgc tcgcatcggc cggcagtgtc cgggtcaccg cgatcaggaa gtgcgcgacc
     4141 gcgataccgc ccacgacagg ctttttcagg gccagcgcct tgcccccgcc atcgccatgc
     4201 agcacgacgt tctcgtcgag aagcgaaacc agccttgcgg tgtcgccgcc tcgcgcggcg
     4261 tcggcgaagc tgcgcaacag cgcgcgatgg cgctcacggt cgacggcaaa ccgcggccgc
     4321 tgctcgatca tgtgcgcgcg cgcccggctg aaaatcttgc gacaggcgac ggcatctcgc
     4381 tgaaccaccg gggcgatctc ttcgaaggaa agatcgaacg cgttgcgcaa cacgaacacg
     4441 acgcgctcag ccggagagag gcgttcgagc acgacgagca gggcgaacga tacgtcttcg
     4501 gcacgaacgc cgggttcgcc ctcggtcaag aggggctccg gcagccactc gccgacatag
     4561 gtttcgcgcc gcgcatgctg atcgcggagc cgattgaggc tgagccgcgc ggtgatggtc
     4621 gcgagatagg ccttgagcga gcgcaacgca tcctttggct tggcatcatg caggcgcacg
     4681 aaggcgtcct gcacgatctc ttcggcgtca gcccggttgc cagtgatccg gtaggccagc
     4741 gcgaacatgg ccggacgcag ctcggcgaag gcgcgatcga gggtcacgcc gtctggacct
     4801 tttgggccgt ccggacccgc tgtatcgctt cgtttgcctg gtgccatgat actcgccgcc
     4861 gcccgggcca gaaaaagaag cccggtactc tacgttcgat tttgagcgcg taactgatga
     4921 accacacgaa aaaattacgg actatgcgtg ccgttcgacc tgtcaggata aaccacgctt
     4981 gtttgtcgtc gggatagctc ggaaatccga cgccaccgcg accgattgca atgccttggc
     5041 cgaaggtaga aaagctgaac ggcgcgagct tccgcttcgc tctctgcgca aggatcgcat
     5101 ccgcggcgta ggcgcccgat gttatcgcaa cgaatgcaga caatcggtag ggggcgccag
     5161 tcggtgcgac tggatgcgcg gcatctccga cggcaatgat atgcggatga gaaatcgacc
     5221 gcaggttggc atccacccag atccggcccc gcgggtcggt cgcgacgccg gcgccactgg
     5281 cgaccggcgc cgagcgcaat ccgcccgacc acacgcagat atcaaaggga acacgccgac
     5341 ccttgtcggt cacgatctca tccaaatgca cctcggcgac cgtctcgctg tcgatcattc
     5401 tcacgcccaa ccgcccaagc tccgtacgca cggcgcgttc cacgcgcgct cccttgaaat
     5461 caccgcatcg tgagctgctg atcatggtca tctccacact cggccacgcg gtcttgattt
     5521 cgccggcgac ctcgatcgcg gtttcggcgc cgcccaccgt gatgacgcgc atcggccggc
     5581 tggcattgtc ctgcaatctc gatcggagag ccgacgttga acgcgggcct tcggcagcct
     5641 ccagacgata ggcatgctcg gctgcgcccg gaacagcgtc cgcgtcgacg cgtgaaccca
     5701 gcgcgtagat gacctcatcg aacattatct cccgctgctg cgcgccggac gtgatccgaa
     5761 cgcagcgtcg gtcggcatcc agcgaagcca cttcgccaca gaagaattcg atgccggtgc
     5821 cttcaaggaa cgcccaaatc gacgggatcc gtggcgcgac cggcgccaca atgctctcct
     5881 gcagccgcac gcgttccaga aactgatcgg aagcattgac gagcgcgatg cgcagttctt
     5941 gctttttttg agtgagtctg agcgcggcca tgaggccggc atagccggcg cccaaaatca
     6001 caatgtcgta agccgggaaa tgtcccgtcg tctctgtcgc gtccatacga atctccactg
     6061 atcggcgtcc tgaagaacag gacacgccag gaggctgaaa cgtgacaacc ggggctgcgt
     6121 cgaagcctgc tatttatcag gatcggaggg cgccatcggc tcgtgaatgc gccttgatga
     6181 agtcgacgaa ggccttgaga gccgcaggaa cttgccttcg actgggataa tagagataga
     6241 aacctaccga gcgcggcgcc caatcttcca gcagcggtac gagccgcttc ttgccgacgt
     6301 gagcgtcgat cctgctgagc ggcaggcggg ctatgcctat gccgtccagc gccgcgcgga
     6361 gcgcgaggtc gccatcgctg acgatgagcg agcctttgac caggacctcc atgctctttc
     6421 cgcgcttttc gaacgcccag ggttgcatgg cgccgctcgc aaagcgcatg cggatgcaat
     6481 tgtgtgactg cagatctcca ggaatcttcg gagcggggtg tcggcgcaga taatccggag
     6541 atgcgacgat ggtggggcgc gcgtcctcgc cgacgcgaac cgcgatcata tcccgtccca
     6601 cgcgatggcc gggccggatg ccggcgtcga agcgatcgcg aacgatgtcg gtcaatccgc
     6661 tgtcggtgat gatctcgatg ctgatggcgg gatatgcggc cagaaacttc gagaggatgg
     6721 gctcgatcac cgtcttcgcg gccggacgtg gaacggtgag acgaagctgg ccccccggct
     6781 tgtcgcggaa cacgttgacg gactccacgg cggcttcaaa actgtcgagc gccggccgca
     6841 actccgccag caggcgctcg ccgacctccg tcagcgccac gctccttgtg gtccgattga
     6901 gcagcctcac gccgagtttt tcttcgaggc cgcggatggt ttcgctgagc gccgagcgcg
     6961 aaatgccgag ataaaccgcc gccttggcga agctggagcg ttccgcggtc gccacgaagg
     7021 cgttcatctc ggcgaacccc aacttccgca ttgatcggaa tctccgacta tctcatgcgg
     7081 ataatgccag attgtcgaat aaacatcaaa gttttatttc tccggcagat cagaacgtgg
     7141 ctcgttacga cttccagctt tggcggtcgt ccggtttctg ccgcgctttc gacggagaac
     7201 ctgtgatgac cgtggtttcg cgctatcaca cgttgttggt ggccctgcac tggatactgg
     7261 ctgtgctgat catcgccgcg ctggccctgg gtgcgctggt aatggtcaag attccaaaca
     7321 gcgatcccat gaagctcgag gcgcttcgca gccacatgat cggcggttca ttgatcgcag
     7381 tcctgatgct cgttcgtctt gtcgtgcgga tgcgaaccga gcatccggca acgccgtcgg
     7441 ctggaaatcc tgtgttggac cggttggcat gggcttcgca ccggttgctg tatgtggccg
     7501 tgctcggcca ggcggcgagc ggtgtcatga tggcgctgca gaccggcttg ccgggcatcg
     7561 tctttggcgg cggcgccttg cccgccgatt tctgggtcta tccgatccgg acggtgcatt
     7621 atcttttctc gcggacgctg atggcactca tcgcgctcca cgtcgcaggc gcgctctacc
     7681 acacgctggt gctgcgggac ggattgctga gacgcatgtt cttcggccgc cgcatcgtcg
     7741 cagcggccga ttccagaacg gctctcacca aaccgattcc ggggacaagg ccatgaacta
     7801 cctgacgccg gcgcaggcat ccgcccaatt catgagtctg atcgtgctgg ccgcggtggc
     7861 gcgatggcac atcattccct ggctcaaccg ccgtgcacgt gcggacgcgc tgatcgcgct
     7921 gctctgggtg catgtgttcc gctatgtcgc attgcagatc ttctccgcgc agcatgacgg
     7981 ctttccgatt tcggacggcg gagcgatgga gatcgtggtc ggcgacgtcg ccggcgccgt
     8041 gattgctttc atcgcgatca cgctgcttcg gcgccgggcg cggcttggaa ttcttctggc
     8101 gtggctgctg gcggcggaaa cggcctacga cactgtggcc aacatccacg gcggcgttca
     8161 agagcatctc atgggcgccg ccagcggcgt gacgtggatg atcttgacct tcttcgtgcc
     8221 gatggtggtg gtgagcggag ggctgatgat ctggcagctt tatgcgagga ggggtgagga
     8281 actcggctct tcccaagagc gcggtgtgct gccaagcgac gcgcctctcc gcagggcgtt
     8341 ctaggcatta tctcggaggc ctacggcgcc gagtcagtcg acgctgacgc cgctggcctt
     8401 gatcggcacc gcccatttgg cgatctcgct ttgcacgaat ttcgccagat attccggcgt
     8461 ggtgcgatcg tcggagacga atttgagccc ggcgctttcc agcttctccc gcacggccgg
     8521 cgttttcatt gcctcgatcg tcgcatgatt gagcttttga acgatggcgt ccggcgttgc
     8581 ctttggcagg aaaaacgcgt tccaggtgta ggcgttgacg tcaaaaccct gctcaacggc
     8641 ggtgggaacg tttggtagca ccggcgagcg ctgtttggcc aggatggcga tggctttcac
     8701 cgcgccaccg tcgatctgcg gcttcgcggt ggtgatgata tcgcagagat agtcgacgcg
     8761 gccgctcatc agatcctgca tcgccggatt ggcgccgcgg tagggaacgt ggacgatatc
     8821 gagcccggcc accatgttca tcagcgcgca accgagatgg atcgccgaac cggtgccggc
     8881 ggaaccgaac tgcatcttgt ccttgttggc tttggcgtag gcgacaaatt ccttcatgtc
     8941 cttgac
//
LOCUS       DASWGM010000204         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_848255,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000204 DASWGM010000000
VERSION     DASWGM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848255"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1237
                     /locus_tag="VGA15_06320"
     CDS             101..1237
                     /locus_tag="VGA15_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9587345.1"
                     /translation="MPTAPLLTATPSGDGLELRPGGSWTAANVTTLETLSNAVTAQLD
                     RARTVKVDMAEVRELDTLGAWLLEKMSRRVTSAGHRADVVGIADNYTGLIEEVRQVNR
                     HNPAPAPARNPVVVRVDEIGRSAVGATEDVAVFLQMLGSLFLAILGLLRRPRSLRVTS
                     LVYQLYRVGWLAIPIVVLITFLIGAIIAQQGIFHFRKFGADSYVVDMVGILVLRELGV
                     LIVAIMVAGRSGSAYTAELGSMKMREEIDALSTMGLDPVEVLILPRVIALVCALPILT
                     FIGSMAALYGGGLVAWFYGGMGPAIFIARLHDAISVTSFQVGIIKAPFMALVIGVVAC
                     SEGLRVKGSAESLGKQTTTSVVKSIFLVIVLDGLFAVFFASIGM"
     gene            1240..>1640
                     /locus_tag="VGA15_06325"
     CDS             1240..>1640
                     /locus_tag="VGA15_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9587346.1"
                     /translation="MQESAPQFAIRVSNLVVGFGKHVVIDHLSLDVRRGEILGLVGAS
                     GGGKSVLMRTIIGLIPRQGGAIEVMGSAIGGTHDRVTQSAAGKWGILFQQGALFSSLT
                     VRQNIQFPLRENLVLSDALMDEIATAKLEMVG"
BASE COUNT          274 a          491 c          546 g          329 t
ORIGIN      
        1 gcggcgcacg ggacccgcag ggtgtcttga ggcaggtcaa cgtgagtaca gcggccgggg
       61 cttaaacaaa ccagcgttcc catcgaagag gatacagatc ttgccgaccg cacccctgtt
      121 gacggccacg ccatcaggcg atgggcttga gttacggcca ggcgggtcgt ggaccgcggc
      181 caatgtaacg acgctggaga cgctctcgaa cgccgtaacg gcgcagcttg atcgggcgag
      241 gaccgtgaag gtggatatgg ccgaggtgcg cgagctcgac acgctcgggg cctggctgct
      301 tgagaaaatg tcgcggcggg taacctcggc cggccatcgc gccgacgtcg tcggcattgc
      361 cgacaactat accggcctga tcgaggaggt ccgtcaggtc aaccgtcaca atcccgcgcc
      421 ggcgccggcg cgaaatcccg tcgtggtcag ggtggacgaa atcggccgct ccgcggtcgg
      481 ggccaccgaa gatgtggccg tattcctgca gatgctgggc tcgctttttc tggccatcct
      541 cggcctgctg cgcaggccga gatcgctgcg ggtgacatct ctggtctatc agctctaccg
      601 tgtcggctgg ctggccatac cgattgtcgt gctgatcact ttcctgatcg gggccatcat
      661 cgcgcagcag ggcatcttcc atttccgaaa attcggcgcg gactcctacg tcgtcgacat
      721 ggtcggcatt ctggtgctgc gcgagctcgg ggtgctgatc gtcgccatca tggtcgccgg
      781 ccgctccggc agcgcctaca ccgccgagct cggctcgatg aaaatgcgtg aggaaattga
      841 cgccctgtcc actatgggcc ttgacccggt cgaagtgctg atcctgcccc gcgtcatcgc
      901 gctggtttgc gcgctgccga tcctgacctt catcggatcg atggccgcgc tctacggcgg
      961 cggtctggtg gcatggttct atggcggcat ggggccggca atttttatcg cgcgactgca
     1021 cgacgccata tccgtgacca gcttccaggt gggcattatc aaggcgccgt tcatggccct
     1081 ggtgatcggc gtcgtcgcct gcagcgaagg cctgcgggtg aaaggaagtg ccgagtcgct
     1141 cggcaaacag acgacaactt cggtggtgaa atcgatcttt ctggtgatcg tcctcgacgg
     1201 gctgtttgcg gtctttttcg catcgatcgg catgtgacga tgcaggaaag cgccccgcag
     1261 tttgcgattc gcgtcagcaa ccttgtggtc ggcttcggca agcatgtcgt gatcgatcat
     1321 ctgtcgctgg acgtgcgccg aggcgagatt ctgggcctcg taggcgcgtc cggcggcggc
     1381 aagtcggtgc tgatgcgcac catcattggc ctcattccgc ggcagggcgg cgcaatcgag
     1441 gtgatgggct cggcgatcgg cggcacccat gatcgggtca cccagagtgc ggcggggaaa
     1501 tggggcattc tgttccagca gggcgcgctg ttttcatcgc tcaccgtgcg gcagaatatc
     1561 cagtttccgc tgcgcgaaaa cctggtgctg tcggacgcgc tgatggacga gatcgccacc
     1621 gccaagctcg aaatggtcgg
//
LOCUS       DASWGM010000205        11035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_983850,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000205 DASWGM010000000
VERSION     DASWGM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11035
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983850"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VGA15_06330"
     CDS             complement(<1..339)
                     /locus_tag="VGA15_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX9587347.1"
                     /translation="MSTISATAVSAPKPLYQSLFVQVLAALVLGIALGVAVPDFAVEL
                     KILSDAFLKLISMIVAPIVFCVVVHGIAGAGDLKKVGRVGVKALIYFEVMTTVALVVG
                     LLLAYLFGPGH"
     gene            554..1288
                     /locus_tag="VGA15_06335"
     CDS             554..1288
                     /locus_tag="VGA15_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX9587348.1"
                     /translation="MIHDVSIPAALIAGLVSFLSPCVLPLVPPYLVYLTGATIEHVVN
                     DETVSSSKRAAMGAAVMFVLGFSTVFVALGASASLIGGLIRAWSGELSIVAGIVIIIM
                     GLHFLGLTRIGFLMREGRLPIPKPVGLWGAYVMGLAFAFGWTPCIGPILAAILSVAAA
                     EATVTKGAGLLAVYSAGLGIPFLIAAFMVEQFSSLFSRMKRHLASVEHAMGVLMVITG
                     IGFLTGAVSNVSIWLLETFPSLQNFG"
     gene            complement(1434..2699)
                     /locus_tag="VGA15_06340"
     CDS             complement(1434..2699)
                     /locus_tag="VGA15_06340"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX9587349.1"
                     /translation="MEEAMNRKSVYGILGVTALGLSLFVGPVRANVITDWDEKAVAVV
                     TPMSSLGGTTPYMAVRMMGMVHAAMFDAVNSIEQRYRPYLVQLPADPATSKEAAAAAA
                     AATVLATIDAKTAGDMKVALATYLAPLPEGVAKSDGVRLGETVAAKVIEARANDGCNA
                     PDAYRPRTSPGVYVPTAITINSMWPDMKPFAIAKGSQFRPKPPIALDSKQWATDYNEL
                     KDYGGKISTKRTAQQTETALFWTSPLLAYQPLVRQLVTAKQMGVADSARFMALEAVAL
                     NDAIIAVLDAKYHYNFWRPITAIRNGDNDGNPATDSDPTWQPMAITPMHPEYPCSHCI
                     QSGSVAAVVKAVLGGTDIPEVALTSPATPGVTHRWTNMTAFTEEIANARIWAGFHYRF
                     STAVGTDMGLQIGEYVVKNVMQPVVTGSR"
     gene            complement(3770..3973)
                     /locus_tag="VGA15_06345"
     CDS             complement(3770..3973)
                     /locus_tag="VGA15_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587350.1"
                     /translation="MNLTHKFAEMTPERRPQDPHMDGTGLRFETMEHGGEYPDTMPQA
                     IKLIDAEDQAPAKLSASQGARWG"
     gene            4210..4374
                     /locus_tag="VGA15_06350"
     CDS             4210..4374
                     /locus_tag="VGA15_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587351.1"
                     /translation="MRQAATLLEFAQSTTNPQLAAVLVEKAAALKSKVDEANARPGLS
                     PLAPDVEPET"
     gene            complement(5234..6742)
                     /locus_tag="VGA15_06355"
     CDS             complement(5234..6742)
                     /locus_tag="VGA15_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9587352.1"
                     /translation="MPKSSLPVRLALLVAGTTLPLIVFATVLVFNDYEQDRRDASRRV
                     LETVRSIRLVLDAEVQRMTGGLQVLTLTDALRNGDFENFRRIASGFLDQYGPDGVVLV
                     ADRQGRQLFSSMTTNTANLPMRHNRDIVEKVFATKSPAYSNLFIGAVKKRPIMTVEVP
                     VLRDGDVLYDISFSPPISIFQTIIEKQRPSADWTISIFDGNGVNFARIPNPHETIGKR
                     ASPSLYAEMLRRPEATLQTVSLEGVPLITSYARSSLTGWTVAAGIAESSLVGPLWRSL
                     AITSVIGSVLLLTGLAFAVRMATTIARGEMLHDLLIDELNHRVKNTLAILQAVAVQTF
                     RSASKAEREKFEGRLGALAEAHNLLSTEKWQGSELQDVVSRVLQPYLLNNPERMRMSG
                     PSVPLSPRFAVVLSMILHEMATNAAKYGALSNDTGTVKLDWEVITEHAGPKLRLVWTE
                     AGGPPVVAPVQRGFGSRLIERSARDQLGGEATVNFLPRGVVYTVTCALEEAG"
     gene            complement(6885..8159)
                     /locus_tag="VGA15_06360"
     CDS             complement(6885..8159)
                     /locus_tag="VGA15_06360"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9587353.1"
                     /translation="MLAPTPASPESAGMSKAALDRIEAHLKHRYVDAGRFPGTQLLVY
                     RRGNIVHSAVQGFADLERKAPMKDDTIFRIYSMTKPITSVAFMMLFEEGRVALDEPVH
                     KYIPEWKNLGVFQAGIWPAFLTRPPSRPMLIVDLLRHTSGLTYGFQQRSNVDATYREL
                     KIGEVVKSGALQSMVDDLSKMPLEFSPGEAWNYSVSTDVIGYLIGKISGQPFEQFLKE
                     RIFTPLGMNDTDFFVPPDKAHRFAACYSADPKGGMTFHATDRKGSLTLQDDPTKSSFL
                     SPPSFISGGGGLCSTSADYLTFCRALLGGGELGGVRLLGPKTLALMTSNHLPGGRDLP
                     EMSRSLFSEATYNGIGFGLGFSVTMDPAKTLIPGSPGEYSWGGAATTSFWIDPAEELI
                     AIFMTQVLPSSAYPLRRELRTMVYAAITDSNL"
     gene            8297..9208
                     /locus_tag="VGA15_06365"
     CDS             8297..9208
                     /locus_tag="VGA15_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX9587354.1"
                     /translation="MPQFNDTELTAAVIRSFDETPDPRVKFLMEELVKSLHEYVRKTD
                     LTFDEWGYAIDFLTRVGQKCTPTRQEFILLSDVFGVSMLVDAVNHRERDGATETTVLG
                     PFYVGEHKPMPHGADISANLPGERMFVQSSVTDLKGKPLAGVPVDVWHADDDGYYDSQ
                     RPSYETDGPSSRARFVTDADGRFFFRTILPCSYPIPTDGPVGEMILQTRRHPMRPAHV
                     HFLVAAPGYEPLITHVFIDGDKYLDTDVVFGVKNELIARIEKRTDPVMPDGKPAAAPW
                     HLMTYDFRIKPGEGAAPKPMMAATEDA"
     gene            9525..10841
                     /locus_tag="VGA15_06370"
     CDS             9525..10841
                     /locus_tag="VGA15_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9587355.1"
                     /translation="MTKPTDQTSRPGLSRRQILKGAAGTAALLAAAKLNFPAGAFAQG
                     AGPEVKAAKLGFIALSDAGPLFVAKDKGLFAKYGMPDVEVLKQASWGTTRDNLVLGSE
                     GNGIDGAHILTPMPYLISSGKVTQNNQPTPMYILARLNLDAQCISVGKEYADLKIGLD
                     TAPFKVALDKKKAAGKSIKAAMTFPGGTHDLWIRYWLAAGGIDPDKDIETIVVPPPQM
                     VANMKVGTMDCFCVGEPWNLQLIHQDIGYTAVNTGEIWNKHPEKSLGMRAAWVDKNPK
                     AAQAITMAVMEAQQWADKAENKEELATIMGKRQWINCPVEDILDRTKGKFDYGTGKVI
                     ENSPHVMKYWSDHASYPFQSHDLWFLTEDIRWGKYEAGYDAKALIAKVNREDIWRDAA
                     KALGVAASDIPTSTSRGKETFFDGKVFDPENPAAYLKSLSIKRVDV"
BASE COUNT         2179 a         3306 c         3371 g         2179 t
ORIGIN      
        1 gtggcccggc ccgaacaggt aggcgagcag caggccgacg accagcgcga ccgtggtcat
       61 cacctcgaaa tagatcagcg ccttgacgcc gacccggccg actttcttga ggtcgccggc
      121 gcccgcgatg ccgtgcacca cgacgcagaa cacgatcgga gcgacgatca tcgaaatcag
      181 cttcaaaaag gcgtcgctca agatcttgag ctcgacggca aaatccggca cggccacgcc
      241 aagcgctatt cccagcacca gagccgccag gacctgaacg aaaagtgatt ggtagagagg
      301 ttttggtgcg cttaccgcag tcgcagagat ggttgacatg acttgctccc cctatcgatt
      361 gggcttactt cgagcaacaa atgtgccaaa acagacggcc cagatgttgt ggtgaaaatt
      421 ccgtaatatc ttcgggattc cagcagcttc ttgcagcttc tgaaagcgtc gagatcggaa
      481 ggtcggtgga tagttcaatt ggacaaggcc cggatcttga cgccggtcct ggtcaacatg
      541 gttcgacgac tgcatgattc acgatgtctc gattcccgcc gcgctgattg ccggccttgt
      601 cagcttcctg tcgccgtgcg tgctgccttt ggtgccgccc tatctggttt acctgaccgg
      661 cgcgaccatc gagcatgtcg tcaatgatga aacggtatcg tcctcgaagc gcgcggcgat
      721 gggtgcggcc gtgatgttcg tgctcggctt ttccaccgtg ttcgtggcgc tcggcgccag
      781 tgcctccctg atcggagggt tgatccgcgc ctggtcgggg gagctctcga tcgtcgccgg
      841 catcgtcatc atcattatgg gcctgcactt cctcggactg acgcggatcg gatttttgat
      901 gcgggaaggg cggttgccta ttccaaagcc ggtaggcttg tggggggcct atgtgatggg
      961 gctggcgttt gcgttcggct ggacgccctg catcggcccg atcctggcgg caattttatc
     1021 ggtggcggcg gccgaggcga ccgtgacgaa aggcgcgggc ctgctcgcgg tctattccgc
     1081 ggggctcgga attccgtttc tgatcgcggc tttcatggtt gaacagttct cgtcgctgtt
     1141 ctcgcggatg aagcggcatc tcgccagcgt cgagcacgcc atgggcgtat tgatggtcat
     1201 caccgggatc ggctttctca ccggcgcggt atccaacgtc agcatctggt tattggagac
     1261 gtttccaagc ttgcagaatt tcggctgagg cgcggactca tcaaggacgt cgcgcccgcg
     1321 atgcaggctc caaggggtaa ctcgtgcgaa agctgtggac cacttgtcgc tgggatcgtg
     1381 tcgccaacgg aactacgccg gactcgctag tggacgcccg gcgcagcaac cgttcaccgt
     1441 gaacccgtca caacgggctg catcacgttt ttcacaacgt attcgcctat ctgaagcccc
     1501 atatccgtcc cgacggcggt cgagaagcgg taatggaagc cagcccagat gcgagcattt
     1561 gcaatctctt cggtaaaggc cgtcatgttg gtccagcgat gggtgacgcc gggggtcgcc
     1621 gggcttgtca gcgcgacctc ggggatgtcc gtgcctccca gcaccgcctt gacgacggct
     1681 gcaacgctac cgctctgaat gcaatgcgaa cacgggtatt ccgggtgcat cggcgtgatg
     1741 gccatcggct gccaggttgg gtcactatcg gtggccgggt tgccatcgtt atcgccgttg
     1801 cgaattgccg tgatcggccg ccagaagttg taatggtact tggcgtccaa cacagcgatg
     1861 atcgcgtcgt tgagcgcaac cgcctcgagc gccatgaagc gcgcgctgtc tgcgacaccc
     1921 atctgctttg cggtcacgag ctggcgtacc aagggctgat aggccaaaag cggactggtc
     1981 cagaacaggg ccgtctcggt ctgctgggcc gttcgcttcg tactgatctt tccgccgtag
     2041 tccttcagct cgttgtagtc ggttgcccac tgtttgctgt ccagcgctat cggcggcttt
     2101 ggccgaaatt gggatccctt ggctatggcg aacggcttca tgtccggcca cattgagttg
     2161 atggtgatcg ccgttggcac atagacgccc ggcgaggtcc gcggccggta ggcatcgggt
     2221 gcattgcaac catcgttcgc ccgcgcctca atgaccttgg cggccactgt ctcgccgagc
     2281 ctgacgccat ccgatttcgc gacgccctcg ggaagtggcg cgaggtaggt cgcgagggcg
     2341 actttcatgt cacccgctgt ctttgcgtcg attgtcgcca ggaccgtggc ggctgcagct
     2401 gcggcggcgg cctccttcga cgtagcggga tccgcgggca gctgcaccag atacgggcgg
     2461 tagcgctgct cgatcgaatt gaccgcgtcg aacatcgcgg cgtggaccat ccccatcata
     2521 cgcacagcca tgtacggcgt ggtgccgcca aggctggaca ttggcgtaac gacagcgacc
     2581 gctttctcgt cccaatcggt aatgacgttg gcgcgcacgg gcccgacaaa aagcgaaagc
     2641 cctagcgctg ttacaccgag aataccgtaa acactcttcc tattcattgc ttcctccatt
     2701 gcttttcaga tggtcgtgac acggcgcagc ctcaggcgca tccgccgaag gtgcggtcga
     2761 aaatcattta tctgtcaata tttggagtac tcgcccgccg atgggcattc tgcgctgcca
     2821 gtaaaggggc tttcggcacg acctgtagca gctctaaaaa actctacatc agttgccgcg
     2881 tgcgatcttg tatatccttc gctgagtata ttcgatgaaa ggcttccaat tatgaagtgg
     2941 atcacacttc attgctgcta caggtaatcg ggcaggagac gatgcagtgc gtaggatggg
     3001 ttgagctctt cgcgaaaccc atcataggat gccagatcgt aggatgccaa cgggtccgga
     3061 cttcggccgg cccgatgata aactccggta cacccgtcac gtcctcgcat caccatgcca
     3121 gcgtgcccgc atgaccgact atcgtcgtaa cctcattgcc ggggggcagc ttcttgttca
     3181 ctgccaatct ggcggaaggc gatgggtttc gcgaagggct caacccatcc tacgcacttt
     3241 tcggaggtaa gcggtcatgc tgaggatgtc ggctcatcga ggtaaagcgg acctcgccat
     3301 agcgcgcgcc caaatctaaa attcaattta gtggtgtcgg cggattgcca acctacatca
     3361 gcttcacgca aggagctgat gacgtgagtg atcatgataa atggattagc tattcgcatt
     3421 ggggaatgtt cctaactgga acggaatatt cgaaataacc ctctgcgaga gatcagatga
     3481 ttccgcctca gtttggcggt tcccctttga atctgttttt gtgtatcgca gccggaacga
     3541 ttgccgggtt cccgcgtgag tcattggggt tgggacaata gcatcgggaa agtgcagtgc
     3601 gccgcaagca gatcaagttc caatctgaga gcgtcatggc cgatgtttat ctcggcgtaa
     3661 ccgttgcagg cttgtacacc ctgatcggaa tggggctcat tatcatgttt atgtgaaggg
     3721 cccggcctca gttggtggcc tctttcatgt gagccttgac cacggcgcat cagccccagc
     3781 gcgccccttg ggacgcgctc aacttggcag gcgcttggtc ctcggcatcg atcagtttga
     3841 tggcttgggg catcgtgtcg ggatattccc cgccgtgttc catagtttcg aagcgcaggc
     3901 cggtcccgtc catgtgcggg tcttgcggac gcctttccgg cgtcatctct gcaaatttgt
     3961 gagtgaggtt cattctgtcc gcgcaacgaa gcggccccag cctttggggg aagctggggc
     4021 cacaccttgt tgtgcgggga ggggcgatcc cgacacgagt ctgtaatgtt ttgaccacgc
     4081 caactgttcc acgtgttcca caagtacact tttgtacatt tcgcccggaa cgaaatcccg
     4141 gcttctgcgt gatgttttcg gccgggcaat agaagcgggg aacccaacgt gataagcagg
     4201 cagtatttcg tgcgtcaggc ggcgacactt ctcgagttcg cccagtcaac caccaaccct
     4261 cagcttgctg ccgttctggt cgagaaggca gccgccttga aatccaaagt tgatgaagcg
     4321 aacgcgaggc cgggcctcag cccgcttgcg ccggatgtcg aaccagaaac ctaaacgcgt
     4381 cgaatgacca gaggtcgcgc ggctgacggc aaggccttgg aaccctgggc gctaccgaaa
     4441 cttgcctctc tgtcgcgata ccgcgaatcc aaggaggaga aactaaaatg aaacggcttt
     4501 tgttgatcgg atttgctgcc tgcgctgtta cgctcgctcc aactctgtct gcgtcggcaa
     4561 tgcccgccgc aacgccggct acgctgggcg gctcggatgt ggcggtcatt caggtcaaag
     4621 gtggccacgg tcatggtcac ggccatatgg gccggggcgg tcgcggtcat cattacggat
     4681 ggggcagggg tcgagggcac cattacggtt ggcgacacca tcaccactag aaccacacac
     4741 ccggaataat tctagagagg ccgcctcggt tggcggcctc tttcatgtga gtcttgacga
     4801 tggcacatcc tcttggcaca tcctcttggc acatcctctt ggcgcggcct cctgccgccc
     4861 gcgaactttt cctgcaatct agcggcattg cccgcagcag tgcgaagctc agcacggtag
     4921 gcgaaggcgg ggtggccagc cgacgcgaac cagcgtttcg tccggatcgg aaaaaccatt
     4981 gatccccatg acaccaaagt taccccgctc cgcgaacagg tgcgtcgcgc ccggtacggt
     5041 gcgaaccatg tccgagatta cgatccggac atgtagcgga tgtgttaaaa tcgacgcgaa
     5101 tatctagcca agcctgcgat gtcgcttctt ggtacatcgc gacatttcgc tgcgcttgct
     5161 gaatttgatc gctatcgggg cttggcggac atcgagcaag ccgtaccaat cgagttcgat
     5221 tcatgagcgc gccctagcca gcctcctcca gcgcgcaggt caccgtgtag accacgccgc
     5281 ggggaagaaa attgaccgtg gcttcgccgc cgagttgatc gcgcgcgctg cgttcgatca
     5341 gccgcgaccc gaaaccgcgc tgcaccggcg cgaccacggg agggccgccg gcctcggtcc
     5401 agaccagtcg cagttttggc ccggcgtgtt ccgtgatgac ttcccaatcc agcttaacgg
     5461 tgccggtatc gttcgacagc gccccatact ttgcggcgtt ggtcgccatc tcatgcaaga
     5521 tcatcgacag taccaccgca aaccgcggcg atagcggtac gctcggtccg gacatccgca
     5581 tccgctccgg attgttcagc agatagggct gcaacacacg gctgaccacg tcctgcagtt
     5641 cggagccctg ccacttctcc gtgctcagca ggttgtgagc ctccgccagc gcgccgagcc
     5701 ggccctcgaa tttctcgcgt tcggccttgc tggcgctgcg aaaggtctgc accgcgacgg
     5761 cctgcaggat cgccagcgtg ttcttgacgc ggtgattgag ttcatcgatc aacaggtcat
     5821 gcagcatttc gccgcgagcg atcgtcgtcg ccatccgcac cgcgaaggca aggccggtca
     5881 gcagcagaac gcttccgatc acgctggtga tggcaaggct gcgccacaac gggccgacca
     5941 gcgagctttc cgcgatcccg gcggccacgg tccagccggt cagcgaggat cgcgcgtacg
     6001 acgtaatcag cggtacgcct tcgagcgata ccgtctgcag ggttgcttcg ggcctgcgaa
     6061 gcatctcggc atacagcgat ggcgatgcgc gcttgccgat ggtctcgtgc gggttgggaa
     6121 tgcgcgcgaa attgacgccg ttgccatcga agatcgagat cgtccagtct gcgctgggac
     6181 gctgcttttc aatgatagtc tggaaaatcg agatcggcgg gctgaaggaa atatcgtaaa
     6241 gcacgtcgcc gtcgcgaagc accgggacct cgaccgtcat gatcggtcgc ttcttgaccg
     6301 cgccgatgaa caggtttgaa taagctggac tcttggtcgc gaacaccttc tcgacgatgt
     6361 cgcggttgtg gcgcatgggc aagtttgcgg tgttcgttgt catcgaggag aacaactggc
     6421 ggccttggcg gtcggccacc agcacgacgc cgtccgggcc gtattggtcg agaaagccgg
     6481 acgcaattcg acggaaattt tcgaaatcgc cgttgcggag cgcgtcggtg agcgtcagca
     6541 cctggagccc gccggtcatg cgctgaacct cggcatcgag cacgaggcgg atgctgcgca
     6601 cggtttcgag cacgcgccgc gacgcatccc tgcggtcctg ctcgtagtcg ttgaaaacaa
     6661 ggacggtcgc gaaaacgatc agcggcagcg tggttcccgc gaccagaaga gcaagacgca
     6721 ctggcagtga ggattttggc acgcgggtcc tgcatcaagg catttggcgc gggtggcgcc
     6781 aaatcaccac cagcatagga gaaccacctg aaatccgcca aggattttgg gccggcggac
     6841 cggtttcgac gggccttggg tgcgatgcgg aagttgcgcc gctgctacaa attgctgtcg
     6901 gtgatggcag cgtaaaccat ggtgcgcagc tcgcgccgca gcggataggc gctcgacggc
     6961 agcacctggg tcatgaagat ggcgatcagt tcttccgccg gatcgatcca gaacgacgtc
     7021 gttgcagcgc cgccccagga atattcgcct gggctgccgg gaatcagcgt cttggcggga
     7081 tccatcgtga ccgaaaaacc gaggccgaag ccgatgccgt tataggtcgc ctcagagaac
     7141 agcgaccgcg acatttccgg caaatcgcgc ccgccgggca ggtggtttga ggtcatcagc
     7201 gccagcgtct tcggaccgag cagcctgacg ccgccgagtt cgccgccgcc cagcagcgcg
     7261 cggcagaagg tcagatagtc ggccgaggtc gaacacagcc cgccgcctcc ggaaatgaac
     7321 gatggcggcg agagaaacga acttttggtc gggtcatcct gcagcgtcag gctgcctttg
     7381 cggtcggtgg catgaaacgt catgccgcct ttcgggtcgg ccgagtaaca agccgcgaaa
     7441 cgatgagcct tgtcaggggg gacgaaaaag tcggtgtcgt tcatgccgag cggggtgaag
     7501 atgcgctctt tcagaaactg ttcgaatggc tggccgctga tcttgccgat gagatagccg
     7561 atcacatcgg tggagacgga ataattccag gcctcgccgg gcgagaattc cagcggcatt
     7621 ttggagaggt cgtcgaccat cgactgcagc gctcccgatt tgacgacctc gccgattttc
     7681 aattcgcggt aggtggcatc gacgttggag cgctgctgaa agccataggt caggcccgag
     7741 gtatgccgca gcaggtccac aatcagcatc ggccgcgatg gcggcctggt gagaaatgcc
     7801 ggccagatcc cggcctggaa cacgccgagg ttcttccatt ccgggatgta tttatggacg
     7861 ggctcgtcga gcgcgacgcg gccttcctcg aacagcatca tgaaggcgac ggaagtgatc
     7921 ggtttggtca tcgaatagat gcgaaagatc gtatcgtcct tcatcggcgc cttgcgctcg
     7981 aggtcggcaa agccctgcac cgcgctgtgg acgatgttgc cgcggcggta caccagaagc
     8041 tgcgttcccg ggaagcggcc ggcatcgacg tatcggtgct tcagatgcgc ctcgatgcgg
     8101 tcgagggcgg ccttggacat gccggcggat tcgggcgagg cgggggtcgg ggctaacatc
     8161 ggacgggtct ccgggggcga gggttgcctt gatagccgaa aagtgtgtcc tgttccaatc
     8221 gcagcccggc ttaccccggg ctatcgactg gtgtaggctt gcagccctgg atcgcgtacc
     8281 ccgcaggaca atgaccatgc cccaattcaa cgacaccgaa cttaccgccg ccgtgatccg
     8341 cagctttgac gagacgccgg acccgcgggt caaattcctg atggaggaac tggtgaagtc
     8401 gctgcacgaa tacgtgcgca agaccgacct caccttcgac gaatggggct atgcgatcga
     8461 tttcctgacc cgcgtcggcc agaaatgcac cccgacccgg caggaattca tcctgctgtc
     8521 ggacgtattc ggggtttcga tgctggtcga tgccgtgaac catcgcgagc gcgacggcgc
     8581 caccgaaacc accgtgcttg gcccgttcta tgtcggcgaa cacaagccga tgccgcacgg
     8641 cgccgatatc tccgccaatc tgccgggcga gcggatgttt gtgcagagca gcgtgactga
     8701 cctcaagggc aagccgctcg ccggcgtccc ggtggacgtc tggcacgccg acgatgacgg
     8761 ctattacgat tcgcaaaggc cgtcctacga aaccgacgga ccgtcatcgc gagcgcgctt
     8821 tgtcaccgac gccgatggaa ggttcttctt tcgcaccatc ctgccatgca gctatccgat
     8881 cccgaccgat ggtccggtcg gcgagatgat cctgcagacc cggcgtcatc cgatgcggcc
     8941 ggcgcatgtg cattttctgg tcgcagcacc cggctacgag ccgctgatca cccacgtctt
     9001 tatcgatggt gacaaatatc tcgataccga cgtggtgttc ggggtcaaga acgagctgat
     9061 cgccagaatc gagaaacgca ccgatccggt gatgccggac ggcaagccgg cggccgcccc
     9121 gtggcatttg atgacctatg atttccgcat caagccgggc gaaggggccg cgccgaagcc
     9181 gatgatggcg gcgaccgagg acgcctgaaa aacgccgcct cgacgcctgc tcatttcttg
     9241 tgcggcgcac aagaaatgag cgaatcgcaa attttcggtg cggccgcagg tgaggcgagc
     9301 tgccagaggt aatacctaac cctttatata ttttgctctt tttgcgattc agtggcttgg
     9361 cacgaagctt gaatagcctg tgctgacgcc attgaagccg tccctcgaaa accaatcatc
     9421 cgaactttga caccacgaga ctggggcctc cccagagcgc gcccctgtgc gcctcgcgcg
     9481 atgccaccgg acggccgtac agacccaaag gacgacagca acccatgacg aagcccaccg
     9541 atcagacctc ccgccccggc cttagccgcc gtcaaatcct taagggcgcc gcgggaacgg
     9601 cagcgctact ggccgccgcc aagctgaatt ttcccgccgg cgccttcgcg caaggcgcgg
     9661 gcccggaagt gaaggccgcc aaactcggct tcatcgcgct gagcgatgcc ggcccgctgt
     9721 tcgtggccaa ggacaagggt ctgttcgcca aatacggcat gccggacgtc gaggtgctga
     9781 agcaggcatc gtggggcacg acgcgcgaca acctcgtgct cggctcggaa ggcaacggca
     9841 tcgatggcgc gcacatcctg acgccgatgc cgtatctgat ttcgtccggc aaggtgacgc
     9901 agaacaacca gccgaccccg atgtacatcc tggcgcggct caatctcgat gcgcagtgca
     9961 tttcggtcgg caaggaatac gccgacctca agatcggcct cgacaccgcg ccgttcaagg
    10021 tcgcgctcga caagaagaaa gccgccggca agtcgatcaa ggccgcgatg acttttccgg
    10081 gcggcaccca tgatctgtgg atccgctact ggctcgccgc cggcggcatc gatccggaca
    10141 aggacatcga gaccatcgtg gtgccgccgc cgcagatggt cgccaacatg aaggttggca
    10201 ccatggattg cttctgcgtc ggcgaaccat ggaacctgca gctgatccat caggacattg
    10261 gctacaccgc tgtcaacacc ggcgaaatct ggaacaagca tccggagaaa tcgctcggca
    10321 tgcgcgccgc ctgggtcgac aagaatccga aggcggcgca agcgatcacc atggcggtca
    10381 tggaagcgca gcagtgggcc gacaaggccg agaacaagga agaactcgcg accatcatgg
    10441 gcaagcgcca gtggatcaac tgtccggtcg aagatattct cgatcgcacc aagggcaagt
    10501 tcgattacgg caccggcaag gtgatcgaaa acagcccgca cgtcatgaag tactggagcg
    10561 atcacgcctc ctatccgttc cagagccatg acctctggtt cctcaccgaa gatatccgct
    10621 ggggaaaata cgaggccggc tacgacgcca aggcgttgat cgccaaggtc aaccgcgaag
    10681 atatctggcg cgatgccgcc aaagccctcg gcgttgccgc ctccgacatc ccgacctcga
    10741 cgtcgcgcgg caaggagacc ttcttcgacg gcaaggtgtt cgatccggaa aatccggcgg
    10801 cgtatctgaa gtctctgtcc atcaagcgtg ttgacgtctg atcgaagccg gaccgctcgc
    10861 gcgcttgcgt gcggtccgcc caacgaaccc ggagaaacac tttcgatgaa tatgtccgcc
    10921 atgaagaccg cacccgtcac gagcgttccg gccagttcgg cgccggtggt tgccatgacc
    10981 ccgaagcggc cgccacgcat ggatacatac atcaaggcgg cgaaagaaac cgcgg
//
LOCUS       DASWGM010000206         6434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_983960,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000206 DASWGM010000000
VERSION     DASWGM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983960"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VGA15_06375"
     CDS             <1..744
                     /locus_tag="VGA15_06375"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX9587356.1"
                     /translation="HPTTGTFMWSAPPRERAMLYILEDTVGRNDVLFPRCSAYVYEAS
                     YGFAVHTNCHDIQAEAQREYGLTPDDVHDSFNLFMCTGVDQDGHAFMTRQTTRPGDHV
                     DLLALMDVLAVPNVCGADVMKTSNFALKPLKLTVFAAADADLAKVPNVPKLASQRTPK
                     DFRNPAIKSDRPLRRDPAYRAEFPNTPIVITELPIALEPDELAMFNAVKRTDIYGDDD
                     AAALRDLLFSWWEERFLRAHAGAPSIESK"
     gene            758..1444
                     /locus_tag="VGA15_06380"
     CDS             758..1444
                     /locus_tag="VGA15_06380"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9587357.1"
                     /translation="MDAIAPPDSVTFDFRNLSARDRYRLMIGTVVPRPIALVTTVDPQ
                     GRINAAPFSFFNCLSADPPILALGVENHPDMSFKDTALNIRLTEAFTVNIVSHAIAEA
                     MHICAVPFAAGYDELQAAGLTAMPGTLVASPWIKQAPASFERRRHVTLELGKSRQIIL
                     GEIVFAHYHPDVIDVDRMRIDPAKLDAIARLGGNTCATIRDRFEMPTPTLAEFHASLA
                     VPPDRREAGS"
     gene            1441..2946
                     /locus_tag="VGA15_06385"
     CDS             1441..2946
                     /locus_tag="VGA15_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9587358.1"
                     /translation="MTGRRTVIRGGLVLDAEGRASLRDVLIEDGRILAIDSPGFDVSD
                     DARVLSAADRLLIPGLISAHTHSHGALNRGAVDDKVSLEMFLTGAGASTRSRGLDDKY
                     LSAALSAAEMIRKGCTACFDLSVEFPQASREGISAVARAYRDAGMRAVVAPMIADRTI
                     YQALPGLLDALPVELRSQCAGLAAAPIEQTLSACQDILANWEFDRRWIRPALGPTIPL
                     HCSDEFLTGCADLAREHDVPLQTHLAESRAQAAIGLARYGKSLVGHLEQLGFLSERLS
                     AAHAIWLDDDDIARLGQSGVRVAHNPSSNLRLGSGVAPVRNMLENGIVVGVGTDASNT
                     SDGQNMFEATRLASYLSRIDGFATDAWLSAGDAFHLATEGSAKVLGFEKIGRLAPGYE
                     ADIVFLRLDSPHFVPLRAPLIQMVFGENGASVRTVMIGGRIVFHDGKLLTLDERLLRR
                     QAQQAASRLDQANAETYASAATVARLVGTFCAAQGCTGHALPRKLTLASEG"
     gene            3061..4077
                     /locus_tag="VGA15_06390"
     CDS             3061..4077
                     /locus_tag="VGA15_06390"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HEX9587359.1"
                     /translation="MNGLGGLNKSPDGVVIGLVQLQLPVVVTRADLARQTDRIVYIVG
                     KARRNLGTMDLVVFPEYSLHGLSMDTNPEIMCRLDGPEVAAFKKACIDHKIWGCFSIM
                     EFNPDGNPYNSGLIIDDHGDIKLYYRKFHPWIPVEPWEPGDIGIPVIEGPKGAKIALI
                     ICHDGMFPEMARECAYKGAEIMIRTAGYTAPIRDSWRFTNQANAFQNLMVTANVCMCG
                     SDGSFDSMGEGMIVNFDGSIIAHGTTGRADEIITAEVRPDLVREARIHWGVENNIYQL
                     WHRGYVAVKGGAMDCPYTFMQDMVAGTFRLPWEDQVKVTDGTPCGFPVPTRMFGPKTA
                     KAAE"
     gene            complement(4150..4677)
                     /locus_tag="VGA15_06395"
     CDS             complement(4150..4677)
                     /locus_tag="VGA15_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587360.1"
                     /translation="MTQVLETVMPRLAVHYSVLYLIARMMFCVGGAVAFFFAAGHMRY
                     QNWFTTVGYTVAVILVGVALTCAYRLVTAKGQTVVSIDATGFKDTRLTPTLIPWSAIR
                     SVYAYKDRSRKANRVSLEIDPAFKRGLSIRPGAKLFRWANLSFGSVFDVDIGTLEADA
                     DEISRAAEPYIVKKA"
     gene            complement(4674..5969)
                     /locus_tag="VGA15_06400"
     CDS             complement(4674..5969)
                     /locus_tag="VGA15_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEX9587361.1"
                     /translation="MSEMNAAIAGLQNPSLHPSLGDEIVGRINRLGAISETPEHLARV
                     FLSKEHRAAADLILAWMREAGMQAHLDAIGNVCGRYEGERPGLPCLMLGSHYDTVRDA
                     GKWDGPLGLITAISCVGDLHNGGRRLPFAIEVTGFADEEGVRFASTLLGSRAVAGTFD
                     ESVLGVRDSAGISMRDALVQFGLDPDHIGAAARARSELLAYVELHIEQGPVLEAEGLP
                     VGVVTAIAGATRLAASLTGMAGHAGTVPMPLRRDALAGAAECIVAIEEFCRTDEGGLV
                     GTIGYIHAMPGATNVIPGQVSFTIDMRAPTDAHRKLAVADIVRRIEAIAGRRKLVLQL
                     DVTHENRTVPCAAWLKSQVSEAIAGQGYPVFELPSGAGHDGMAMIDIADVAMVFVRCR
                     GGISHHPDEHVEPADVDAGARVLLRLIENFRPRSAAERM"
BASE COUNT         1245 a         2065 c         1935 g         1189 t
ORIGIN      
        1 catccgacca ccggcacctt catgtggtcg gcgccgccgc gcgagcgcgc gatgctctac
       61 attctcgaag ataccgtcgg ccggaacgac gtgctgtttc cgcgctgcag cgcctacgtc
      121 tatgaggctt cttacggctt cgccgtgcac accaattgcc atgacatcca ggccgaggcg
      181 cagcgggagt acggcctgac gccggatgac gtccacgatt ccttcaacct gttcatgtgc
      241 accggcgtcg accaggacgg ccatgccttc atgacgcgac aaaccaccag gcccggggac
      301 catgtcgacc tgctggcgct gatggacgtg ctggcggtgc ccaatgtgtg cggcgccgac
      361 gtgatgaaga ccagcaattt tgcgctgaag ccgctgaagc tgacggtatt tgccgctgcg
      421 gatgccgacc tcgccaaagt ccccaatgtg ccgaaactcg ccagccagcg cacgccgaag
      481 gatttcagga atccggcgat caagagcgac cggccgttgc ggcgcgatcc cgcctacaga
      541 gcggaatttc ccaacacgcc gattgtgatc accgagcttc cgatcgcgct cgagccggat
      601 gaactcgcca tgttcaacgc ggtcaagcgc accgacattt atggcgacga tgacgccgcg
      661 gcgctgcgcg atctgttgtt ctcatggtgg gaggaacgct tcctgcgggc ccatgccggc
      721 gcgccgtcga tcgaaagcaa gtgacggtga gccaatgatg gacgccatcg cccctcccga
      781 ctccgtgaca ttcgatttcc gcaacctgtc ggcccgcgac cgctacaggc tgatgatcgg
      841 caccgtggtg ccgcggccga tcgcgctggt gaccacggtc gatccgcagg ggcgtatcaa
      901 cgccgccccc ttcagctttt tcaattgcct gtccgcggac ccgccgatcc tggcgctcgg
      961 ggtggaaaac catcccgaca tgtcgttcaa ggacaccgcg ctcaatatcc gcctcaccga
     1021 agcattcacc gtcaatatcg tgtcgcatgc gatcgccgag gcgatgcata tctgcgcggt
     1081 gccgttcgcc gccggatacg acgaactgca ggcggcgggt ctgaccgcga tgccgggcac
     1141 gctggtcgcc tcgccctgga tcaaacaagc cccggcctcg ttcgaacgcc gccgccatgt
     1201 cacgctcgag ctcggcaaat cgcgccagat cattttggga gagatcgtgt tcgcgcatta
     1261 ccaccccgac gtcatcgacg tcgaccgcat gcggatagac ccggccaaac tggacgcgat
     1321 cgcgcggctc ggcggaaaca cctgcgccac gatccgcgac cgtttcgaga tgccgacgcc
     1381 gacgctcgcg gaattccatg ccagccttgc tgttccgccc gatcggcgcg aagcaggatc
     1441 atgaccgggc gccggaccgt cattcgcggg ggcctggtgc ttgatgccga aggtcgcgca
     1501 tcgttgcgcg atgtgctgat tgaggatggc cggatcctcg cgatcgacag tccgggcttc
     1561 gacgtttccg atgacgccag ggtgctgtcg gccgcggacc ggctgctgat tcccggcctg
     1621 atcagcgcgc acacgcattc gcatggcgcg ctcaaccggg gagcggtcga cgacaaggtc
     1681 tcgctggaaa tgttcctgac cggcgccggc gccagcacgc gatcgcgcgg cctcgacgat
     1741 aaatatttga gcgcggcgct ctccgcggcc gagatgatcc gcaagggctg cacggcgtgt
     1801 ttcgatctct cggtggaatt tccgcaagcc tcccgagaag gaatttcggc ggtggcgcgg
     1861 gcctaccgcg atgccggcat gcgcgccgtg gtcgcgccga tgattgccga ccgcacgatc
     1921 tatcaggcgc tgcccggcct cttggatgcg ctgccggttg agctgcggtc gcagtgcgcg
     1981 ggccttgccg cggcaccgat cgaacagacg ttgtcggcat gccaggacat cctggcgaat
     2041 tgggagttcg accgccgctg gattcgcccg gcgctcggcc cgaccatacc gttgcactgc
     2101 tccgatgagt tcctgaccgg ttgcgccgat ctcgcgcgcg aacatgacgt accgctgcag
     2161 actcatctgg cggaatcgcg cgcccaggcg gcgatcggac tggcgcgata tggcaagagc
     2221 ctggtcggac atctggaaca actgggcttt ctgtcggaac gcctgagtgc cgcgcatgcg
     2281 atctggctcg acgacgacga catcgcaagg cttggccagt cgggcgtgcg ggtcgcgcac
     2341 aatccttcca gcaatctccg gctcggatcc ggcgtcgccc ccgtgcgcaa catgctggaa
     2401 aacggaatcg tggtcggcgt cggtaccgac gccagcaaca cctccgatgg acagaacatg
     2461 ttcgaggcga cgcggctggc atcctatttg tcgcgcatcg acggatttgc gaccgacgcg
     2521 tggctttcgg caggcgacgc ctttcatctc gccacggaag gatcggcaaa ggtcttggga
     2581 tttgaaaaga tcggccggct ggcgccggga tatgaggccg acatcgtgtt cctgcggctg
     2641 gacagcccgc atttcgtgcc gctgcgcgcg ccgttgatcc agatggtatt tggcgagaac
     2701 ggggcttcgg ttcgcaccgt catgatcggc gggcgcatcg tctttcacga cggcaaactc
     2761 ctgaccctcg atgaacgcct gctgcggcgg caggcgcagc aagccgccag ccgactggac
     2821 caggccaacg ccgaaaccta tgcgtcggca gcaacagtcg cgcgactggt cggaaccttc
     2881 tgcgcggcgc aaggctgcac cggccatgcg ctgccgcgaa agctcacgct ggcgagtgaa
     2941 ggataacggc ccgcaccgcc tgaaaaatga tcttttgcta ttggacaaaa tctagtttgc
     3001 ttgcggtcgg tatttcacgc cgcgacctca aggggaatca caagtgagaa ggagcttgat
     3061 atgaacggac ttggcggcct caacaaatcg cctgatggcg tcgtcatcgg gctcgttcag
     3121 ttgcaactgc ccgtcgtcgt gaccagggcg gatctcgcgc gtcagaccga ccgcatcgtc
     3181 tacatcgtcg gcaaggcgcg ccgcaatctg ggcaccatgg atctcgtggt gtttcccgaa
     3241 tattccctgc atggcctgtc gatggacacc aatcccgaaa tcatgtgccg gctcgacggc
     3301 ccggaagtcg cggcgttcaa gaaggcctgc atcgaccata agatctgggg ctgcttttcg
     3361 atcatggagt tcaaccccga tggcaatccc tacaattccg gcctgatcat cgacgatcac
     3421 ggcgacataa aactgtatta tcgaaaattc cacccgtgga ttccggtcga accgtgggaa
     3481 ccgggcgata tcggcattcc cgtgatcgag ggaccaaaag gtgcaaagat cgcgctgatc
     3541 atctgccatg acggcatgtt cccggagatg gcgcgggagt gtgcctacaa gggcgcggag
     3601 atcatgatcc gcaccgccgg ctacaccgcg ccgatccgcg acagctggcg cttcaccaac
     3661 caggccaacg cgttccagaa tctcatggta accgccaatg tctgcatgtg cggatcggac
     3721 ggttcgttcg attcgatggg cgaaggcatg atcgtgaatt tcgacggcag catcatcgcc
     3781 cacggcacca cgggacgggc cgacgagatc atcaccgcag aggtacgccc ggatctggtg
     3841 cgcgaggcgc ggattcactg gggcgtcgaa aacaacatct accagctctg gcaccgcggt
     3901 tacgtcgcgg tgaagggcgg cgcgatggat tgtccctaca ccttcatgca ggacatggtc
     3961 gcgggcacct tccggctgcc ctgggaagat caggtcaagg taaccgacgg cacgccttgc
     4021 ggctttccgg tgccgacgcg gatgttcggg ccgaagaccg ccaaggcggc ggagtaatca
     4081 ttcatgtctg cctccccggc cccgccactg cgccagttgc tcgatagccg ctgtacacgt
     4141 cgctgatgct caggcttttt tcacaatgta aggttctgcg gctcgcgaaa tctcatcggc
     4201 gtcagcttcc aatgtgccaa tgtccacatc aaaaactgac ccaaaagaca ggttcgccca
     4261 acgaaagagc ttggcacccg gccggatcga gagacctcgc ttgaacgccg ggtcaatctc
     4321 aagggaaacg cggttcgcct tgcgactgcg gtccttgtaa gcataaaccg atcggattgc
     4381 gctccatggt atgagcgtcg gcgtcaatcg agtgtccttg aagcccgttg catctatcga
     4441 aacgacggtt tggcccttcg cagtcaccaa gcgatatgcg cacgtaagcg ccacgcccac
     4501 caatatcact gcgacggtat agccaacggt ggtgaaccag ttttggtatc tcatgtgccc
     4561 cgcagcaaag aaaaaggcca cagcaccgcc tacacagaac atcatgcggg cgatcaagta
     4621 gagaaccgag taatggaccg caagccttgg catgaccgtc tcaagcacct gtgtcacatt
     4681 cgctccgccg cgctgcgagg acgaaaattc tcgatcagcc tcagcaatac ccgcgcgccg
     4741 gcatcaacgt cggcgggctc gacatgctcg tcgggatgat ggctgatgcc gccgcggcac
     4801 cgcacgaaca ccattgcgac atcggcgatg tcgatcatcg ccatgccgtc gtgccctgcc
     4861 ccgctcggca gctcgaacac ggggtagccc tgccctgcaa tggcctccga cacctggctc
     4921 ttcagccatg ccgcgcacgg cacggtacgg ttctcgtggg tgacgtcgag ttgcagcacg
     4981 agcttgcggc gcccggcgat cgcctcgatc cgccggacga tatcggccac cgcgagcttg
     5041 cgatgcgcat ccgtaggcgc gcgcatgtcg atggtgaacg atacctgccc cgggatgacg
     5101 ttggtcgcac cgggcatggc atggatgtat ccgatggtcc cgaccaaccc gccttcgtcg
     5161 gtacggcaga actcttcgat cgcgacaatg cattccgcag cccccgccag cgcatcgcgc
     5221 cgcaggggca ttggcacggt gccggcgtgg ccggccatgc cggtcaggct cgccgcaagc
     5281 cgcgtggcgc ccgctatcgc ggtcacgaca ccaaccggca gaccttccgc ttccaacacc
     5341 ggcccctgct cgatatggag ttcgacatag gcaagcaatt cgctgcgggc ccttgccgct
     5401 gctccgatat gatcgggatc gagaccgaac tggacgagcg catcgcgcat cgaaattccg
     5461 gcactatccc ttacgccaag cacgctctcg tcgaacgtgc ctgccaccgc gcggctgccc
     5521 agcagcgtgg aggcgaaccg gaccccctcc tcatcggcga agccggtgac ttcgatcgcg
     5581 aacggcaatc gccggccccc gttatgcaga tcgcccacgc aggaaatcgc cgtgatcagt
     5641 ccaagcggac cgtcccattt gccggcatcg cgcaccgtgt cgtagtgcga tcccagcatc
     5701 aggcacggca atcccggtcg ctcgccttcg taacggccgc agacgtttcc gatcgcgtcg
     5761 agatgcgcct gcattcccgc ctcgcgcatc catgccagga tcaggtcggc tgcggcgcga
     5821 tgctccttgc tcaggaacac gcgtgccagg tgctcgggcg tttcggaaat cgctccgagc
     5881 cgattgatcc ggccaacgat ctcatcgcca agggatggat gaagggacgg gttttgcagc
     5941 ccggcgattg ccgcattcat ctcgctcatg tgtttccaat gacaaaagcc acgccgcgcg
     6001 tgcttttcgg ccgatcgata caagttcggt gccagcagcc cggcgaatcc aggaccgttt
     6061 gagtggtacc agcctaagcc agcggggcca gccggcaaat gatcggattg gctacccgga
     6121 aaggttttcc ggagcgtcga tcggagtcaa ggattgctta actcaattgc atacaattag
     6181 ggaatgtcgc ctaaattttt gcccttcatc tccatgcaca agttttgaac cgggagatat
     6241 cccgaagtaa aagctatgta tttcaattag ttacataaat attgcccaca aatcgatcgc
     6301 tggcatgaag attgcgacat gccttgcggg ctccgccgaa acggcgaagc cgcgaaatcg
     6361 gcggcggttc gtccgccaag aggagcaccc catggatttt ggcaggattt cacgacggca
     6421 tctgttgcaa ggaa
//
LOCUS       DASWGM010000207         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_848563,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000207 DASWGM010000000
VERSION     DASWGM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848563"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VGA15_06405"
     CDS             <1..773
                     /locus_tag="VGA15_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9587362.1"
                     /translation="AIVGTKVRLPAADFGRIEETIAKSLEGSLRQLGMERVDIFHLHN
                     AITVEGGGESLGVRQVLDQVVPAFERLRQKGMIRFLGITAVGDTAALRQVIDARVIDS
                     AQVTYNMLNPSAGAALPPRYPAQDYGLLLDHTRAAGVGVIGIRVLAGGALSGSAARHP
                     IASPPPAPIGSATSYDGDLERARRLMPLVEQGFAQNLAEAATRFAISHPAVGTILVGM
                     ATLGQFEAALAAVQNGPLPPAALGRLTDLQQRFSGEAR"
     gene            complement(872..2743)
                     /locus_tag="VGA15_06410"
     CDS             complement(872..2743)
                     /locus_tag="VGA15_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX9587363.1"
                     /translation="MSRSRPGLQRSTSGLAVAALGVVFGDIGTSPLYTLKTVFALTGE
                     HPSPGTILGVLSLVFWTLTIVTTIKYVSVAMSVDNDGEGGILALMALLGVKRARRPVI
                     VAVGLFGAALIYGDGAITPAISVLSALEGLDIATPAVQPYIVPAGVVILLTLFALQRQ
                     GTSRIGRTFGPIMGVWFLCLAVLGIWGIAQYPSVLTALDPRHALRYLFSGETGAFLVL
                     GGVFLCVTGAEALYADMGHFGARPIRLAWSGMVFPSLLLNYAGQAALVLGGAPTNANI
                     FYQLCPSMLLIPMVALATVATIIASQSIITGAFSMTQQAIQLGWLPRFQIVHTSAEGY
                     GQIYVPVVNWLLMMATVALTIGFAKSDNLASAYGIAVSATMLMTSALLFIAMREIWRW
                     NLLASAALAGALMIVDTAFLLANLTKIAEGGYVPLLLAACVYFVMIVWHTGARAVDAR
                     LRDAIMPIEKFMKKVQRDRIARVPGTAVFLTRTQQGAPPVMVWHVKHNRALHRQLFVL
                     STRTRSMPWIGDADRLAFEEVAPNFWRAVADYGFMERPDVPALLKFAHGLGCRIDLDD
                     VTYYVGHETVVPREDRRALPRWLEAMFAFMQRNSAHATDYFRLPSNSVVEIGRQIAI"
BASE COUNT          648 a         1055 c         1013 g          548 t
ORIGIN      
        1 gcgcaatcgt cggcaccaag gtgcggctgc ccgccgccga tttcggccgc atcgaagaga
       61 cgatagccaa atccctcgag ggcagcctgc ggcagctcgg catggagcgg gtggacattt
      121 ttcacctgca caacgccatc acggtcgagg gcggcggcga atcgctcggc gtccgacagg
      181 tgctcgacca agtggtgccc gcgttcgagc ggctgcgcca aaaaggcatg atccgctttt
      241 tgggcatcac cgcggtcggc gacaccgcgg cgctgcgcca agtgatcgac gcgcgcgtca
      301 tcgacagcgc ccaggtcacc tacaacatgc tcaatccttc cgccggcgcc gcgctgccgc
      361 cacgctatcc ggcccaggac tatggcctcc tgctcgacca cacccgggcg gccggcgtcg
      421 gcgtgatcgg catccgcgtg ctcgccggcg gcgcgctgtc gggctccgcc gcgcgccacc
      481 cgatcgcgag cccgccgccg gcgccgatcg gctcggccac gagctatgac ggcgatctcg
      541 agcgcgcccg gcggctcatg ccgctggtgg agcaaggctt tgcccaaaac ctcgccgaag
      601 ccgcgacccg cttcgccatc tcgcatccgg ccgttggcac gatcctggtc ggcatggcga
      661 cgctggggca gttcgaagcg gcgcttgccg ccgttcagaa cgggccgctg ccgccggccg
      721 cgctcggccg gctgacggat ttgcagcaaa gattttccgg cgaggcgcgc tgattttgcc
      781 cgcgctcgcc ggtacgggcc gcgcggcgtc tccgccctcc gcggcatccg ctcgtgcgac
      841 gcaaattcaa cgtggatcag gcgcccggca atcaaatggc aatctggcgt ccgatctcca
      901 cgaccgagtt cgagggcagc cggaagtaat ccgtcgcatg agcggagttg cgctgcatga
      961 aggcgaacat ggcctccagc cagcgcggca gggctctgcg gtcctcacgg gggacgaccg
     1021 tctcgtgacc gacgtaatag gtcacgtcat ccaggtcgat cctgcagccc agcccgtgag
     1081 caaatttcag caacgccggg acatccggac gctccatgaa gccgtagtcc gcaaccgcgc
     1141 gccagaagtt cggagcgact tcttcgaaag caagccgatc cgcgtccccg atccacggca
     1201 tggaccgcgt cctcgtgctc aggacaaaaa gctgccgatg cagcgcccga ttatgtttca
     1261 cgtgccagac catcaccggc ggcgcgccct gttgcgttcg cgtgagaaag accgcggtgc
     1321 cgggcacgcg cgcaatccga tcccgttgga cttttttcat gaacttctca atcggcatga
     1381 tggcgtcacg cagacgcgcg tccacggccc tggctcccgt gtgccataca atcatcacga
     1441 aatagacgca cgcggcgagc agcagcggca catatccgcc ctccgcgatt ttcgtgagat
     1501 tcgccagcag gaatgcggta tccacgatca tcaacgcgcc cgcgagcgca gcggaagcga
     1561 gcaggttcca gcgccagatc tcgcgcatgg cgatgaacag cagcgcagac gtcatcagca
     1621 tggtagccga aaccgcgatg ccgtaggccg acgccaaatt atcggacttc gcaaacccga
     1681 tggtaagcgc gacagtggcc atcatcagca gccagttgac gaccgggacg tagatctgtc
     1741 cgtagccttc ggccgaggtg tgcacgatct ggaatcgcgg caaccagccg agctgaatcg
     1801 cctgctgggt catggagaac gccccggtga tgatggactg gctggcgatg atggtggcga
     1861 ccgtcgccag cgccaccatc ggaatcagca gcatcgaggg acagagctgg tagaagatat
     1921 tggcgtttgt cggcgcgccg ccgagcacca gggcggcttg accggcgtag ttcagcagca
     1981 ggctgggaaa caccattccg gaccacgcga gccggatggg ccgcgcgccg aaatgcccca
     2041 tatccgcgta aagcgcttcg gctccggtga cgcagaggaa gacgccaccg agaaccaaga
     2101 acgccccggt ctctcccgag aacaggtagc gcagcgcatg acggggatcg agtgccgtca
     2161 ataccgacgg atattgtgcg atcccccaga tgccgagcac cgcgaggcac aggaaccaaa
     2221 cgcccatgat cggcccgaac gttcttccga tgcgtgacgt gccctggcgc tgcagggcga
     2281 acaacgtcaa tagaatgacg acccccgcag gtacgatgta gggctgtacc gccggggtgg
     2341 cgatatcgag gccttccagc gcggaaagca cggatatcgc cggcgtgatg gccccgtcgc
     2401 cgtagatcag tgcggcgcca aataggccga cggccacgat cacgggtcgc cgcgcccgct
     2461 tgaccccgag cagggccatc aacgcgagaa tccctccctc accgtcatta tcgacgctca
     2521 tggccaccga aacatatttg atcgtcgtca cgatcgtgag cgtccaaaat acgagcgaga
     2581 ggacgcccag gatggtgccg gggctcgggt gctcgcctgt gagcgcgaaa acggtcttca
     2641 gggtgtagag cgggctcgta ccgatatcac caaagaccac ccccagggct gcgactgcca
     2701 gtccggaagt gctccgctgc agcccgggac ggctgcggct tatggtttgg ctcctgggca
     2761 aatgcgatct tccatttcgc cgggtcggcg tggcggctca catggcggag cacggtgcgc
     2821 cgctcgccgg atcaggcaac acgataatga tggctctgaa aacccttctc tgttgtccga
     2881 ggccggcatt gctgcatcgt ggccgtgatg cttggggcgc acacctatat cgatcggtgc
     2941 gggaacgtca cggcggcaaa agcggactgc agatccgatt cagcaggaca aactgggacc
     3001 agtttttggt aagtctttga aacgatagtc tttttggccc caaattcagt gtttgcgcac
     3061 acacaataag tccagttgaa gatatcttat ttgataacta tacttcaata tatgaaacgc
     3121 cgcatcctca tgcaaaagtt ggacgatatg gatcgacgcg gggtgtgggc cttcaccccg
     3181 gccacatttt cagccctgct tggcgagccc gaacgaaact atctcaagct aatgatgaag
     3241 cgcttggccg accagggcgt actg
//
LOCUS       DASWGM010000208        11140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_18461,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000208 DASWGM010000000
VERSION     DASWGM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18461"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VGA15_06415"
     CDS             complement(<1..423)
                     /locus_tag="VGA15_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587364.1"
                     /translation="MFIGMAHSAMAQSEIKIGEINSYSLLPAFTEPYRKGWQLAVEEI
                     NAAGGINGKKLVVVSKDDGGKPADAQTAANELVSSENVAMLAGAFLSNIGLAVSDFAN
                     QKKVFFLAAEPLTDAITWAKGNRYTFRLRPSNYMQAAML"
     gene            complement(496..1080)
                     /locus_tag="VGA15_06420"
     CDS             complement(496..1080)
                     /locus_tag="VGA15_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEX9587365.1"
                     /translation="MSAIIRKIVTVVEETRLEMGQAVTPPTRRAAAIAVIENPFAGKY
                     VEDLSPLIAIGEELGELLSKKAVAALGIDGTKVQSYGKAAAVGENGELEHAAAILHPK
                     MGAPLRNVLGKGAALIPSSKKRSGPGTTLDIPLGHKDAAFVRSHFDGMEVQINDAPRA
                     NEIMVAVAITDSGRPLPRVGGLTIAEIKGEDGLR"
     gene            complement(1098..2009)
                     /locus_tag="VGA15_06425"
     CDS             complement(1098..2009)
                     /locus_tag="VGA15_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HEX9587366.1"
                     /translation="MTRVPQIGWLADGRRLHLQDGPIDLIVEVHGSETNRRAAYDAAA
                     WRFTGLLDELCDELPLLRQAADPARRELQGAVARRMDAAVAPFALDHFITPMAAVAGS
                     VAEEILGAMLDAARLERSYVNNGGDIALHLGHGEHFTVGLMDRPDRHGLIRTVIVDAD
                     DPTRGVATSGRHGRSFSLGIADAVTVLARTASQADAAATIIANAVDLPGHPAIVRCPA
                     HDLQPDSDLGARLVTRDVGELTGHEIQDALESGAACARKLLAAGLIDGAALRLQGETV
                     VVSARAIDTLRSGTPVHRGAMESAMHV"
     gene            complement(2006..3457)
                     /locus_tag="VGA15_06430"
     CDS             complement(2006..3457)
                     /locus_tag="VGA15_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HEX9587367.1"
                     /translation="MPTDATAAADDKIRCDACPVMCYIKPGAAGACDRYANHNGELVR
                     VDPHIVLERTVSHGGRLVPFQASGDWDGKIVHEPSMFVTAIGAGTTYPDYKPAPFIVS
                     SQIDGVDMVTVVTEGIFSYCGVKVKIDTDRYLGPETATVRAQGEAIGHVTTGEYGSQM
                     LSLGGVHHLTGGSKKEGRVTCDALMDLSNCRPVELTIDGGATVVVQAGYPPIVNGVAE
                     ERMRVGCGSATIGMFAKQWHGKVDEVVVVDDHITGVLSEHQAGKLLDIPDTGIKMKGR
                     RSTPGRYFQVAEPGTGWGGTMISDPLSVLGPFDPKAARPGMTMLMVSTTGEHAAYYEL
                     DAALKPIEKPMPVDLKLSVERIQENCEPALCTVLFMGGAGGSLRAGVTDNPVRLTRSV
                     KDALTRVTSGGAPVYVWPGGGITFMVDVTRLPAGAFGYVPTPALVAPIEFTLKLRDYA
                     ALGGHMDYVRPLSSLKDNTEIRQMPQSPSGQLP"
     gene            3805..4287
                     /locus_tag="VGA15_06435"
     CDS             3805..4287
                     /locus_tag="VGA15_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9587368.1"
                     /translation="MTQTAIRLTVNGRIHDVSAAPDAALLYVLRNDLELNGPKYGCGL
                     GECGACAVLIDGVAARSCVIPISGCVGRDIVTLEGLGSRDHPDPVQDAFIREQAAQCG
                     YCLNGMIMTTKALLMRTPHPSEADVLEALRYNLCRCGAHIEIVRAAMRAAGLVAEVQG
                     "
     gene            4287..7850
                     /locus_tag="VGA15_06440"
     CDS             4287..7850
                     /locus_tag="VGA15_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9587369.1"
                     /translation="MAAPEPPINPELRPGSLSIVRRVGSGAEAGFETFIKITADGSVT
                     AFNGHVDLGTGIRTALGQIVAEELDVSFARVVVVLGDTSRVPNQGATIASETIQITAM
                     PLRKAAAQARQFLIARAAERLELSVDDLAIEDGLIRGRDNRSISYGELIAGENVHLEL
                     ADHVPVKAVDSYTIVGQSVPRTDLLAKATGELVYVHDVRVPGMLHGRVVRPPYAGVDA
                     GAFVGTSLIAVDEASVADVPGLVAVVTIGDFVGIIAEREENAVKAAAQLKVIWKATPT
                     LPDLKDIETALRAHPSAPRTLMDKGDVDAAIAAAAKPMPRTYIWPYQMHGSIGPSCAV
                     ADYQEGHVRVWSGTQNPHILRTDLALLLRRPEPEIEVIRMEAAGCYGRNCADDVSADA
                     VLLSRAAGRPVRVQLTREQEHAWEPKGTAQLMDVRGGLNADGSVAGYDFATRYPSNGA
                     PTLALLLTGAITPVAAVSEMGDRTAIPPYDYDAMRVVAHDMPPIVRASWFRGVSALPN
                     TFAHESYIDELATEAGVDPIEYRLRYLRDPRAIDLVNAVAERAGWTPRPVWKEPIVEG
                     DIVRGRGFAYAQYVHSKFPGFGAAWSAWIADVAVNKATGDVSVTRVVAGQDSGLMINP
                     DGVRHQIHGNVIQSTSRALMEQVSFDRNAVTSREWGAYPIITFPDIPRIDVLMLPRQD
                     QPPLGVGESASVPSAAAIANAIFDATGVRFRELPFTPERILAGLRGEQHLAASALPQT
                     PQQPPASTDKWQNPFVRRRGAFASAAALVAALVGVAAAVLPWRSIAPIARPDASVFSA
                     ATIARGRQLAALGDCAVCHTKADGVINAGGRAVETPFGIIYSTNITPDVETGLGAWSY
                     PAFERAMREGIHRDGRHLYPAFPYPHFAKATDADLQALYAYLMAQAPVRADNPKTTLA
                     LPFNWRPLMAGWNALFHRTGVFEAYSTKSEIWNRGAYLVEGLGHCGACHSPRNALGAE
                     RASAYLAGGFAEGWEAPPLTSLSHAPIPWSEDELFAYLRTGESRFHGVAAGPMAPVVK
                     ELAGLPDQDIRAMAVYLSAFNTEAINKPGQDELATKLETLTGTKVASASALGARLYQG
                     ACAVCHQVGGAPLFGSRPSLALNSNLHSAVPDNLIQVILHGIASPASSDLGYMPAFKN
                     SMTDDQLAELVSYLRQQFAPDKPPWTEVRAAVGRARQAISR"
     gene            7911..9215
                     /gene="pncB"
                     /locus_tag="VGA15_06445"
     CDS             7911..9215
                     /gene="pncB"
                     /locus_tag="VGA15_06445"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX9587370.1"
                     /translation="MAVTDIASRTYNHGWRLDPIVRSLLDTDFYKLLMLQMIREFYPD
                     TRTTFSVINRSRHVRLAEVIDEEELRAQLDHARTLRFSKKELIWLAGNTFYGKTQMFS
                     PDFINWLATFRLPEYELHKVDGQYELHFHGPWMHTTMWEIPALAILNELRSRQVMKGQ
                     GRFALDVLYARAKAKLWAKVERLRKLEDLRLSDFGTRRRHGFLWQRWCVEAVKEGLGP
                     SFTGTSNVLLAMDNDLEAIGTNAHELPMVAAALANDDTELRWAPYRILDQWRHTYGGN
                     LLIALPDAFGTNSFLRDAPEWVADWTGFRPDSAPPIAAGEEIIKWWKQKGRDPKEKLL
                     VFSDGMDVGSIEETYRHFAGRVRISFGWGTNLTNDFMGCAPDGSTNLDPISIVCKVTS
                     VDGRPAVKLSDNPEKATGSASEVERYLRVFGSAGRVRSAVHV"
     gene            complement(9802..9990)
                     /locus_tag="VGA15_06450"
     CDS             complement(9802..9990)
                     /locus_tag="VGA15_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587371.1"
                     /translation="MDKLVEIRNQEFICRERALLDSEHRVFWLTKAEEWERRALDEIA
                     FHFRERNPDRVDLRTATH"
     gene            complement(10181..>11140)
                     /locus_tag="VGA15_06455"
     CDS             complement(10181..>11140)
                     /locus_tag="VGA15_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587372.1"
                     /translation="GIARKWFDVENVDMIANLINSSIALGVTQLAKDKDRIAIVNGSG
                     ASRLTGDACTPNSIHYAYDTYALAKGTGTALMKAGGTSWYFLTADYAFGHALEADTAA
                     VIKSLGGEVLGSTRYPVETSDQSSFLLQAQASRAKVVGLAGSGTVFVNAVKAAQEFGI
                     QQGGQTLAGLLVWITDIKSLGLEVAQGLILTDAFYWDRDDETRAWSKRFSERMKRAPH
                     MGDAGDYSSTMHYLRAIEAAGTDDAKAVMAKMRKMPINDFFAKNGRIREDGRMVHDMY
                     VYEVKKPSESKGEWDYYKLRAVIPGEEAFRSLKDSACPLVKKG"
BASE COUNT         2122 a         3576 c         3307 g         2135 t
ORIGIN      
        1 cagcatcgcc gcctgcatgt aattcgaagg acgcagacgg aaagtgtagc gattgccctt
       61 ggcccaggtg atggcgtcgg tcagaggctc ggcggcgagg aagaaaactt ttttctgatt
      121 ggcgaaatcg ctgaccgcca gtccaatatt ggacaggaac gcgccggcga gcatcgccac
      181 gttctcgctc gataccagtt cgttggcggc ggtctgcgcg tcggcgggtt tgccgccgtc
      241 atccttggag acgacgacga gcttcttgcc gttgatgcca ccggcggcat tgatttcctc
      301 gaccgcgagc tgccagccct tgcgataggg ctcggtgaat gccggtagca gcgagtagct
      361 gttgatttcg ccgatcttga tctcgctctg cgccatggcg gaatgcgcca tgccgatgaa
      421 catgatcgcg agcccggcac ctaagaaaat tctgcttcgt tgcattccaa aatctccaat
      481 gtttgaagaa accagttatc gcagaccgtc ttcacccttt atctcggcaa tcgtcagccc
      541 gccaacgcgc ggcaacggtc tgccgctgtc ggtgatcgcc accgccacca tgatttcatt
      601 ggcacggggc gcatcgttga tctgcacttc catgccgtcg aagtggctgc gcacgaacgc
      661 cgcatccttg tggccgagcg gaatatccag cgtcgttccc ggtccgctcc gttttttcga
      721 cgatggaatc aacgctgcgc ctttgcccag cacgttccgc agcggcgcac ccatcttcgg
      781 atgcagaatt gccgcagcgt gttcgagttc accgttttcg ccgaccgccg ccgccttgcc
      841 ataactttgc accttcgtgc cgtcgatccc gagcgcggca acggcctttt tcgaaagcag
      901 ctcgccgagt tcctcgccaa tggcgatcaa tggcgaaaga tcttcgacat atttcccggc
      961 aaatggattt tcgatcaccg cgatcgccgc ggcgcgccgg gtcggcggcg tgaccgcctg
     1021 ccccatctcc agccgcgtct cctcgacgac cgtgacgatt ttgcgaatga tcgcgctcat
     1081 cgtgtcagcc tcgttctcta gacatgcatc gcactttcca tcgcgcctcg gtggaccggc
     1141 gttcccgatc gaagcgtgtc gatcgctctc gccgatacca caacagtctc gccctgcaaa
     1201 cgcagtgcgg cgccgtcgat caatcccgcc gcgagcagct tgcgcgcgca ggccgcgccg
     1261 gattcgagcg cgtcctggat ttcatgtcct gtcaattcgc cgacgtcgcg ggtgaccaag
     1321 cgggcgccga gatcgctgtc gggctgcaga tcatgtgccg gacagcggac gatggcggga
     1381 tgtccgggca aatcgaccgc gttggcaatg atggtggccg cggcgtcagc ttgcgatgcc
     1441 gtccgcgcca gcacggtgac ggcgtcggcg ataccgaggg agaaactgcg tccgtggcgt
     1501 ccgctggtgg caacccctcg cgtgggatcg tcggcatcga cgatgacagt tcgtatcagg
     1561 ccatgccggt caggccggtc catcaggccc acggtgaaat gctctccgtg gccaagatgc
     1621 agcgcgatgt cgccgccatt gttgacatag ctgcgttcaa gccgcgccgc atccagcatc
     1681 gcgccaagaa tttcttccgc gaccgagccc gcgaccgcgg ccatcggcgt aatgaaatga
     1741 tcaagagcaa acggcgccac ggccgcatcc atgcggcgtg cgaccgcgcc ttgcagttcg
     1801 cgacgcgctg ggtcggccgc ctggcgcaac agcggcaact cgtcgcagag ttcgtcgagc
     1861 agacctgtaa aacgccacgc cgcggcgtcg taggcggcac gccgatttgt ctcgctgccg
     1921 tgaacctcca cgatcaggtc gatcggaccg tcctgcagat gaagccgcct gccgtccgcg
     1981 agccagccga tctgcggaac tctcgtcatg ggagctgtcc cgaaggagac tgaggcattt
     2041 gacggatttc ggtgttgtcc ttcagcgacg aaagtgggcg cacgtaatcc atatgaccgc
     2101 ccagcgccgc ataatccctg agttttagcg taaactcgat cggcgcgacc agcgccggcg
     2161 tcggaacata accgaaggcg ccggccggca agcgcgtgac atccaccatg aaggtgatgc
     2221 cgccacccgg ccagacatat accggggcgc cgccgctcgt gacccgcgtc agcgcgtcct
     2281 tcaccgatcg cgtcaggcgc actggattgt cggtgacacc cgcccgcaac gatccgcccg
     2341 ccccgcccat gaacaacacc gtgcacagcg ccggttcgca attctcctgg atccgctcga
     2401 ccgacagttt tagatcaacc ggcatcggct tctcgatcgg cttcaacgcc gcgtcgagtt
     2461 cataataggc cgcatgttcg cctgtcgtgc tcaccatcag catggtcatg ccgggccgcg
     2521 ccgccttcgg atcgaacgga cccagcaccg aaagcggatc ggagatcatc gtaccgcccc
     2581 agccggtgcc gggctcggcc acctggaaat agcggcccgg tgtcgagcgc cgccccttca
     2641 tcttgatccc ggtatcggga atatcgagca gtttgccggc ctgatgctcc gagagaacgc
     2701 cggtgatgtg gtcatccacg acaacgactt catcgacctt gccgtgccac tgcttggcaa
     2761 acatgccaat cgtggccgag ccgcaaccga cccgcatgcg ctcctcggcc acgccattga
     2821 cgatgggcgg atagccggcc tgcaccacca cggtggcgcc gccgtcgatc gtcaattcca
     2881 ctggcctgca gttgctcaga tccatcagcg cgtcgcaggt gacgcggcct tctttcttgg
     2941 aaccgccggt cagatgatga acgccgccca gcgacagcat ctgcgagccg tattcgcctg
     3001 tggtgacgtg gccgatcgcc tcgccctgcg cgcgaaccgt tgccgtctca ggaccgagat
     3061 aacggtcggt gtcgattttc accttgacgc cgcaataact gaagatgccc tcggtcacca
     3121 cggtgaccat gtcgacgcca tcgatctgcg aagacacgat gaagggcgcc ggcttgtagt
     3181 cgggataggt ggtgccggcg ccaatagcgg tcacgaacat gctcggctca tgaacgatct
     3241 tgccgtccca gtcgccactg gcctggaacg gcaccaaccg tccaccgtgg gacaccgttc
     3301 gctccagcac gatatgagga tcgacgcgca ccaactcgcc attgtgattg gcatagcgat
     3361 cacaggcacc tgccgccccc ggcttgatgt agcacatcac cgggcaggca tcgcagcgga
     3421 tcttgtcgtc agcggcagcc gtcgcatcag tcggcatgaa caagctcaca agccagcgtc
     3481 aaactgtcga taagccaaat cggtaacgac gcctcgtatc gaagttcggg cggcgcctca
     3541 atataacggt tctctcgcca ccggtcattt tgttcgtata cgaacgatgt tgctcgcgca
     3601 gcggaaacct gtcaagcacc gatggacccc gaaaatgccg ctgccgaata aacccgcagg
     3661 ttgcccggca tctctgttca taaaccgggc agcaggttcg ccgcagggcg gtgcgcttgc
     3721 acgtttgtat gcaaacggta tccttgcccc accggttcaa cgcgatgttt gcagcacaac
     3781 catttggccc ccgagaccac cagcatgacg cagaccgcga tccgcctgac cgtcaacggc
     3841 aggatccatg acgtcagcgc cgcccccgac gccgcgctgc tttacgtgct gcgcaacgat
     3901 cttgaattga acggaccgaa atatggctgc gggctcggcg aatgcggcgc ctgcgcggtg
     3961 ctgatcgacg gcgtggcggc gcgttcctgc gtgatcccga tcagcggctg cgtcgggcgc
     4021 gacattgtga cgctcgaagg ccttggctcg cgcgaccatc ccgatccggt gcaggacgct
     4081 tttataaggg aacaggccgc gcaatgcggc tactgcctca acggcatgat catgaccacg
     4141 aaggcgctat tgatgcgcac ccctcaccct tccgaggcgg acgtgctcga agcgctcaga
     4201 tataatttat gccggtgcgg cgcgcatatt gaaatcgtgc gcgcggcgat gcgggcggcg
     4261 ggacttgtcg cggaggtgca gggttgatgg cggcgccaga acccccgatc aatcccgaac
     4321 tccggcccgg gtccttgtcc atcgttcgcc gggtcgggtc aggcgcggaa gccggattcg
     4381 aaacctttat caagatcacg gccgacggtt cggtcacggc cttcaacggg catgtcgatc
     4441 tcggaaccgg cattcggacg gcgctcgggc aaatcgtcgc cgaggaactc gacgtgtcct
     4501 ttgcgcgcgt cgtcgtagtg ctcggcgaca cctcacgggt tcccaaccag ggcgcgacga
     4561 tcgccagtga aaccatccag ataacagcga tgcccttgcg caaagcggcg gcgcaggcgc
     4621 ggcaattcct gatcgcgcgc gccgccgaac gactcgaact ttccgtggac gaccttgcga
     4681 tagaagacgg cctgatccgc ggccgggata atcgcagcat cagctatggc gaattgattg
     4741 ccggcgagaa cgttcacctg gaactggctg atcatgttcc ggtaaaagcg gtcgattcct
     4801 acaccatcgt cggccagtcg gtgccgcgta ccgacctttt ggcgaaggcc accggcgaac
     4861 tggtttacgt tcacgacgtg cgcgtacccg gcatgctcca cggccgcgtg gttcgtccgc
     4921 cctatgccgg cgtcgatgcc ggggcgtttg tcggcacgag cctgatcgcg gtcgatgagg
     4981 cctcggtggc ggatgttccg gggctggtcg ctgtcgtcac gatcggcgat ttcgtcggca
     5041 tcatcgccga gcgcgaggag aacgcggtca aggctgcggc gcagcttaag gtgatttgga
     5101 aggcgacgcc gacactgccc gatctgaagg acatcgagac cgcgctgcgc gcccatccct
     5161 cggcgccgcg gaccctgatg gacaaaggcg acgttgacgc tgcgatcgcc gccgccgcca
     5221 agccgatgcc gcggacgtat atctggccgt accagatgca cggttcgatc ggcccgtcct
     5281 gcgctgtcgc ggattatcag gaaggtcacg tacgggtctg gtccggcacg caaaatcccc
     5341 acatcctgcg caccgacctc gcgctgctgc tgcgaaggcc ggaacccgaa atcgaagtga
     5401 tccggatgga agctgccggt tgctatggcc gcaattgcgc cgacgacgtt tccgccgacg
     5461 cggtgctgct gtcgcgcgcg gccggccgtc ccgtccgggt gcagctgacg cgcgagcagg
     5521 aacacgcctg ggagccgaag ggcaccgcgc aactgatgga cgtcaggggc ggactaaacg
     5581 ccgacggcag tgtcgccggc tacgattttg ccacccgcta tccatccaac ggcgcgccca
     5641 cgctggcgtt gctcttgacg ggtgcgatca cgccggtcgc ggcagtgtcc gagatgggcg
     5701 accgcaccgc gatcccgcct tacgattacg atgccatgcg cgtggtcgcg cacgacatgc
     5761 cgccgatcgt gcgggcatcc tggttccgtg gcgtctcggc gttaccgaat acctttgcgc
     5821 atgaatccta catcgatgaa ctcgccaccg aagccggcgt cgatccgatc gaataccgcc
     5881 tgcgttatct cagagatccc agggcgatcg atctcgtcaa cgctgttgcc gagcgggccg
     5941 ggtggacccc tcgcccggtc tggaaagagc cgatcgtcga gggtgacatc gtgcgcggac
     6001 ggggttttgc ctatgcgcag tatgttcaca gcaagtttcc aggttttggc gcggcgtggt
     6061 cggcctggat tgccgacgtc gccgtcaaca aggcaaccgg cgacgtcagt gtgacccgcg
     6121 tggtcgccgg ccaggattcc ggcttgatga tcaatccgga cggggtccgc caccagatcc
     6181 atggcaacgt catccaatcg accagccgcg cgctgatgga gcaggtctcg ttcgaccgca
     6241 acgcggtgac gagccgcgaa tggggcgcct accccatcat caccttcccg gacattccaa
     6301 ggatcgacgt cttgatgctg ccgcgacagg atcagccgcc gctcggcgtc ggcgagtccg
     6361 cgtcggtccc gagtgctgcg gcgatcgcca atgcgatctt tgatgcgacc ggggtgcgct
     6421 ttcgcgaatt gccgttcacg ccggaacgta ttcttgcggg attgcggggc gaacaacacc
     6481 tggccgcaag cgcattgccg caaacgccgc agcagccccc tgcttcgacc gacaaatggc
     6541 aaaatccgtt tgtcaggcgg cgtggcgcgt ttgcaagtgc ggcagcactg gttgccgcgc
     6601 tggtcggcgt tgccgcagcc gtgctgccat ggcggtcgat tgcgccgatc gcgcggcccg
     6661 acgcatcggt gttttcggcc gcaaccattg cccgcggccg gcaactggcc gcccttggcg
     6721 actgcgcggt ctgccacacc aaggccgatg gcgtcatcaa tgccggaggc cgcgccgtcg
     6781 agacgccgtt cggcatcatc tacagcacca acatcacgcc cgatgtcgaa actgggctag
     6841 gcgcatggtc ctatcccgcc ttcgagcgcg cgatgcgcga gggcatccat cgcgacggcc
     6901 ggcatctcta tccggcgttt ccctatccgc atttcgccaa ggccacggat gccgatctgc
     6961 aggcgctcta cgcctatctg atggcacagg cgccggtgcg tgccgacaac cccaaaacca
     7021 cgcttgcgtt gccattcaat tggcgtccgc tgatggccgg atggaacgcg ctgtttcatc
     7081 gtacaggcgt attcgaagcc tattcgacga aatcagaaat ttggaatcgc ggcgcctatc
     7141 tggtcgaagg cctcggccat tgtggcgcct gtcattcgcc gcgcaatgcg ctcggcgcgg
     7201 aaagggccag cgcctatctg gccggcggct tcgctgaagg ctgggaagcg ccgccgctga
     7261 cttcgctttc gcacgcgccg atcccctgga gtgaggacga attgttcgcc tatttacgaa
     7321 caggcgaatc ccgctttcac ggtgtcgccg cgggcccgat ggcaccggtg gtgaaagaac
     7381 tcgccggtct acccgaccag gacattcgcg ccatggcggt ctacctcagc gcattcaaca
     7441 ccgaggcaat caacaaacca ggccaggacg aactggccac caaactcgaa accttgaccg
     7501 gcacaaaggt cgcttcagca tcggcacttg gcgcgcggct ctatcaaggc gcctgcgcgg
     7561 tttgccatca ggtcggaggc gcaccgctgt tcggtagccg gccgtcgttg gcgctgaaca
     7621 gcaacttaca tagcgccgtg ccggacaact tgatccaggt gattttgcac ggcattgcat
     7681 cgccggcgtc gagcgatctc ggctacatgc ccgccttcaa aaacagcatg accgacgatc
     7741 agctcgccga gcttgtgtcc tatttgcgcc agcaattcgc tcccgataag ccaccctgga
     7801 ccgaagtgcg cgcggccgtc ggccgggcca gacaagccat atcgcggtga cctgccatgc
     7861 ccgacgataa gccactggct gcaaggcttc cggcgagata gaattccacc atggcggtca
     7921 cagacattgc ttcccggacc tataatcacg gctggcggct cgacccgatc gtgcgcagcc
     7981 tgctcgatac cgatttctat aaactcttga tgctgcaaat gatccgggaa ttctacccgg
     8041 acacgcgcac caccttttcg gtcatcaatc gcagccgtca cgtccgtctc gccgaggtca
     8101 ttgacgaaga agagctgcgc gcccagctcg atcacgcccg taccctccgg ttcagcaaga
     8161 aggagctgat ctggctcgcc ggtaatacgt tctacggcaa gacccagatg ttttcgcctg
     8221 acttcatcaa ctggttagcg acgtttcgtc ttcccgaata cgagctgcac aaggtggatg
     8281 gccagtatga attgcacttc cacgggccgt ggatgcacac caccatgtgg gagattccgg
     8341 cgctggccat cctcaatgag ctgcgctcgc gccaggtgat gaaaggacaa gggcgatttg
     8401 cgctcgatgt tctctatgcg cgcgccaagg ccaagctgtg ggccaaagtg gagcggctgc
     8461 gtaaactgga agacttgcgg ctttccgatt tcgggacgcg gcggcgccat ggtttcctgt
     8521 ggcagcgctg gtgcgtcgag gccgtcaagg aaggcttggg gccgtccttc accggcacct
     8581 ccaatgtcct gctggcgatg gacaacgatc tggaagcgat cggcaccaat gcgcacgaat
     8641 tgccgatggt cgcagcagca ctggcgaacg acgacaccga attgcgctgg gcgccgtatc
     8701 gtattctgga tcaatggcgt catacctatg gcggcaatct tctgatcgcc ctgcccgacg
     8761 ccttcggcac caattcattc ctcagagatg cccccgaatg ggtagcggac tggaccggct
     8821 ttcgccccga cagcgcgccg ccgatcgcgg ccggcgagga aatcatcaag tggtggaagc
     8881 aaaaaggccg agatcccaag gaaaagctgc tggttttctc tgacggcatg gacgtcgggt
     8941 cgatcgaaga gacctaccgc catttcgccg gccgggtccg gatcagtttt ggctggggca
     9001 ccaacctcac caatgatttc atgggctgcg cgccggatgg ttccaccaat ctcgatccga
     9061 tctcgatcgt gtgcaaggtg acatcggtcg acggcagacc cgccgtaaaa ctctcggaca
     9121 atccggaaaa agccaccggc agcgcgtccg aagtcgagcg ctatctgcgg gtatttggca
     9181 gcgccggccg ggtgcgcagc gcggtgcatg tctgaaatat cggcacattt ctcctgcggc
     9241 ccagccacga acgccgatcc tcctcttcga ttcaggaatc ttcagcgatt aggtggggtt
     9301 agcaaggcaa gtcatacgca gcccgcatgg actaggatgt tgcggaaata tcacacgcgc
     9361 gaagattttg cggtcgttct tcatacgttc gcaagagttg atggtgggca cacgtcatgc
     9421 gcggtaagtt gaccttgggc tgaaatcgac cgtgctgccg ctgaaggaga gcctacatgc
     9481 cagcctacgt ccagcaccat gacgttgaga acgttgcgat tttctgcccc agttgcatag
     9541 gattacagcc tatgcatgtg agagacgtcg agcctcattg gagcatggcg aagatcgagt
     9601 tcatctatga gtgctccgac tgcggcagcg aagtcaggtt gaccgtaagc aagccggaac
     9661 gccaacactg acgttcggta cctaccaaac gccgtctcgc cgcgactaac ctacactcaa
     9721 tggtgcgcca gcaaatagcc gctaccgagc ctccagcgaa caatcgattg gcgcagcgac
     9781 aaagcttcga gcttccgggc tttagtgggt agctgtccgc aaatcaacac gatcagggtt
     9841 gcgctctcga aagtgaaacg cgatctcgtc aagcgcgcgc cgctcccact cttccgcttt
     9901 ggtcagccag aaaactctat gctcggaatc cagtaacgcc ctctcccggc atatgaattc
     9961 ctggttgcga atttcgacga gcttgtccat gaactccact cccgcgatgt gaagccaatt
    10021 taagcacgca tcatagcaga gttgggaccg gagtctttca atttactttg gaaaggggca
    10081 gcgctcttgt cgctacgata tcgcaggcac cgcagtgcaa cacagtgtcc gtgacgtcca
    10141 tcccggcatc gcacggacgg cgatcaaggc accaaggcag ttagcccttc ttgaccagcg
    10201 gacaggcact gtccttgagg gaacggaacg cctcttcgcc gggaatcaca gcacgaagct
    10261 tgtagtaatc ccattcaccc ttcgattccg acggcttctt gacctcatac acgtacatgt
    10321 catgcaccat ccggccgtct tcgcggatgc ggccgttctt cgcaaagaag tcgttgatcg
    10381 gcatcttacg cattttggcc atcacggcct tcgcgtcgtc ggttcctgcc gcctcgatgg
    10441 ccctaagata gtgcatggtc gaggagtaat cgcccgcgtc gcccatgtgg ggtgcacgct
    10501 tcatccgttc agaaaaacgc ttcgaccatg cacgcgtctc atcgtcgcga tcccaataga
    10561 aggcatcggt aaggatcaaa ccctgcgcca cttcaagccc gaggctcttg atgtccgtga
    10621 tccaaaccag aagacctgcg agcgtctgcc caccctgctg gatgccgaac tcctgcgccg
    10681 ccttgacggc gttgacgaag accgttccgg agcccgccag ccccaccacc ttggcgcgcg
    10741 acgcctgcgc ctgcagcaga aaagacgatt gatccgatgt ctcgaccgga taccgggtcg
    10801 atcccagcac ctcgccgcca agcgacttga tgacggccgc cgtatcggct tcgagcgcgt
    10861 gaccgaacgc atagtccgcg gtgagaaaat accagctcgt tccgccggcc ttcatcaacg
    10921 cggtgccggt gcctttcgcc agcgcatagg tatcgtaggc gtagtgaatg ctgttcgggg
    10981 tgcaggcgtc acccgtcaga cgcgaagcgc cggacccgtt gacgatcgcg atcctgtcct
    11041 tgtcctttgc cagctgggtt acgcccaggg cgattgacga gttgatcagg ttcgcaatca
    11101 tgtcgacgtt ttcgacatcg aaccatttgc gcgcgatccc
//
LOCUS       DASWGM010000209         5863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_292145,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000209 DASWGM010000000
VERSION     DASWGM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292145"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..674
                     /locus_tag="VGA15_06460"
     CDS             390..674
                     /locus_tag="VGA15_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587373.1"
                     /translation="MKTTIIALSAAALIAAAPAVLAQGVTSKAPGLQHKVSKKHHPGV
                     SGYAALREMQAKGSKTGDPGASSYAPDERSRFLDSVRQGGGGGGGGGSGM"
     gene            1239..1661
                     /locus_tag="VGA15_06465"
     CDS             1239..1661
                     /locus_tag="VGA15_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587374.1"
                     /translation="MLAARSFAHSWFGILAVITLVGCASKPPEEANKPAVQTYAEDYQ
                     GLFQRVSTTAKRCFASKAGLYRSGAVDADLYSEIGYGQLTLTVNGSGSSNYYVSVRIE
                     KQPTGSKLTMISDNMGASQRYRELVSGWANGDQNCPAV"
     gene            complement(1829..3124)
                     /gene="rhaI"
                     /locus_tag="VGA15_06470"
     CDS             complement(1829..3124)
                     /gene="rhaI"
                     /locus_tag="VGA15_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HEX9587375.1"
                     /translation="MTDFSISEDLLQAENAARKPALDEDYAALLRALGRRGIDGDALT
                     RKVMAFGVAIPTWGVGTGGTRFARFPGPGEPRHLFDKLEDCATIHHLVRATPTISPHF
                     PWDATSDYVALREVAAKHGLAFDAVNSNTFQDQPNQEHSYKFGSLSHVEPAVRAHAIA
                     HNIDCISIGRQLGSKALTVWIADGSNFAGQSNMTLALDRYLDSMREIYAALPADWRVF
                     LEHKLYEPAFYSSVISDWGTSFAAAQELGPKAQCLVDLGHHAPNVNIEQIVARLVRFG
                     KLGGFHFNDSKYGDDDLDTGSVEPFRLFLVFNELIDARMRGAEGFAPAYMLDQSHNVT
                     DPIESLMQSAIELQRAHAQALIVDRSALAAAQAANDALGAHLELKRAYTTDVSPILAM
                     ARLRAGGAIAPIETYRASGYRKHKVKERPPVAGTSAGIV"
     gene            complement(3149..5245)
                     /locus_tag="VGA15_06475"
     CDS             complement(3149..5245)
                     /locus_tag="VGA15_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEX9587376.1"
                     /translation="MTKAAAEPLPDLWNDAHAADLDEPGLLLYRSNLLGSDLRITNFG
                     GGNTSAKLAMTDPLTQEPITVLWVKGSGGDLGSMKRDGFSTLYLDKLESLKQLYRGIA
                     HEDEMVALFNHCTFNLNPRATSIDTALHGFVPHPHVDHVHADAVIAIAACSNAEELTG
                     KVFGGTIGFLPWQRPGFDLGLKLGAMAAQHPEYVGVVLGGHGLFSWGPTSKAAYQTTL
                     RIIQQAADWLAGHEKQPAFGGPRCDAAAPETCRAVAAALMPLIRGKISRDERKVGHFT
                     DAPEVLEFVNSRALDRLAPLGTSCPDHFLRTKIRPLVLPYDRQSDNVDTLANSLDALL
                     GAYRDDYSAYYQRCKRPNSLAMRDPNAVIYLVPGIGMFSFAKDKATARIASEFYVNAI
                     NVMRGASGVNAYVGLPEQEAFDIEYWLLEEAKLQRMPKAKSLAGRIAYVTGGAGGIGG
                     ATALRLLGEGACVVLADIDQEALDARIQAIVQKFGRDSAVGIPLDVTEEASVVASFHE
                     SILAYGGIDIVISNAGIASASPVEDTSLALWQKNMDVLATGYFLVAREAFRLMQRQQC
                     GGAIVFVASKNGLAASAGASAYCAAKASEIHLARCLALEGAPHGIRVNTVNPDAVLRG
                     SKIWAGEWRQQRAASNKVSEDELEEVYRQRSMLKLSVLPEDIAEGVYFFASDLSAKST
                     GNILNVDAGNPQAFTR"
     gene            5435..>5863
                     /locus_tag="VGA15_06480"
     CDS             5435..>5863
                     /locus_tag="VGA15_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEX9587377.1"
                     /translation="MHERERWQVIRAMLKQQLLVRIADVCRATDASEASVRRDFARLA
                     EMGSATRVRGGLEAMPGSQMASSDAPALATRSFEISKTLNVAAKRAIARAAVALCSDG
                     DAIIINGGTTTFEMGEFLRERRLKVLTNSYPLAETLIHDSN"
BASE COUNT         1120 a         1754 c         1867 g         1122 t
ORIGIN      
        1 gaacgcccag ccgacacgcg tcgaatcatt tgatcgaagc gcgttagcgt aacttctcga
       61 accgccgtat acggacccgt acgtatggtg gtgtgggagg ggaggagccg cgaggctccc
      121 ccctatcccg atccgtggca cctttgagac tgcccagatg tccgcagtcc tggcgctttc
      181 gtgggggaag cggacgtagc ttggacagcc tatttcggta gcgattgacc catatgcgac
      241 atcggccgat agcccaaaca cagcgcgatt agccggtttc aaaggtgagt tctacgcctt
      301 cactgtagcg tgatgtggcc cgttcacgga acgggtctaa tttcagtgtg ccgggcgggc
      361 atttcattcc aaggagggag aactgaaaag tgaagacaac gatcatcgca ctttctgccg
      421 ccgctctcat cgccgccgca ccggccgtgc ttgcgcaggg tgtaacaagc aaagcaccgg
      481 gtctgcagca taaggtctct aagaaacatc accccggcgt ctccggctac gccgcgctgc
      541 gtgagatgca agccaagggc tcgaagacgg gcgatccggg agcttccagt tacgcgcctg
      601 acgaacgcag tcgttttctc gacagtgtga gacaaggtgg tggtggcggc ggaggcggag
      661 ggagtggcat gtgagccctc gcgccggacg aacaggcggt atccgtcctg cttaatcggt
      721 cgggactttg gtggcacggg ttgggatgcc taagcgcggc aagatgccac gacccgccgc
      781 cagtgccgtg tccggtcgcg ctttgatgcg cgaacaaatc aacattccgc atggacaaca
      841 tccggcgcac ggctacagcc gattcaatgc tctgctgtca actctaggca gctttgcggg
      901 ttcggcctgc aacgtttcga ttttggcctt cagggggatg acagcctggt ggccggccgg
      961 taacctggcc ggcagagggg catggttgat ttcggcgaga tcgacgctga ccagcgcgtt
     1021 gccgtcattg ccccgcgatc ggggtaaccg gagccgcttg gcaggcgccc gggcggcctc
     1081 agttgagact ttgagtcggg cgtagtaaac acaccgtgag atgtgggatc agcaggtggg
     1141 gatagtcggc gttgcgcttg cgaattccga caatccgatg atagtccgat gcgccgagaa
     1201 tgtgctcgcg ttgtcgtcgg ttggagtatg agcattaaat gctggcggct cggagctttg
     1261 cgcattcctg gttcggcatt ctagcagtga tcaccttggt cgggtgcgca agtaagccgc
     1321 cggaagaagc aaacaaacca gctgttcaaa cgtacgctga agattaccaa ggtctctttc
     1381 aacgcgtatc gaccaccgcc aagcggtgtt tcgcctcgaa ggctggcctt taccggtccg
     1441 gcgcggtcga tgcggatctt tactccgaga tcggatacgg ccagttgacg ctaaccgtga
     1501 atggcagtgg cagctccaac tactatgtct ccgtccggat cgaaaagcag cccactggct
     1561 ccaagcttac aatgatctcc gataatatgg gggcaagcca gcgctacaga gaattagtat
     1621 cggggtgggc gaatggcgac caaaactgtc cggctgttta gctttcatgc gcgaccgcca
     1681 acagcaggcc cgcgcgacac gattttcgcc tcaccgagct ccccgaccta acctccagcc
     1741 atgcgttttt cggcgcagtc atcaagactc cgaagcgcgc cgaatcccct caggttgctg
     1801 cgctcgcgcc gccaccggcc acgccgcctc aaacgatccc cgccgacgtc cccgccaccg
     1861 gcggtcgctc cttgactttg tgcttgcgat agcccgaggc gcgataggtc tcgatcggcg
     1921 cgatggcgcc gccggcccgc agccttgcca tggccaggat cggtgagaca tcggtggtgt
     1981 aggcgcgctt gagttcgagg tgggcgccga gcgcgtcgtt ggccgcttgc gccgcggcca
     2041 acgctgagcg gtcgacgatc agcgcctggg catgggcgcg ctgcaattct atcgccgatt
     2101 gcatcaagct ctcgatcgga tcggttacgt tgtgcgattg gtcgagcata taggccgggg
     2161 cgaagccctc tgccccgcgc atccgagcgt cgatcagttc gttaaacacc agaaacagac
     2221 gaaacggttc gaccgatccg gtgtcaagat catcgtcgcc gtatttcgaa tcgttgaagt
     2281 gaaagccgcc gagttttcca aagcgcacga ggcgggcgac gatctgttcg atattgacgt
     2341 tcggcgcatg atggccgagg tcgaccaggc attgcgcctt cggtccaagc tcctgcgccg
     2401 ccgcaaagct ggttccccaa tcggaaatca cgcttgaata gaacgccggc tcgtacagct
     2461 tgtgctcgag aaacaccctc caatccgcgg gcagcgccgc gtagatctcg cgcatcgaat
     2521 cgagatagcg atcgagcgcc aaggtcatgt tcgattggcc ggcgaaattg gagccgtcgg
     2581 cgatccacac tgtcagcgcc tttgagccga gctggcggcc gatggagatg caatcgatgt
     2641 tgtgtgcgat cgcgtgggcc cgcacggcgg gttcgacatg ggagagcgag ccgaacttgt
     2701 acgaatgctc ctggttcggt tgatcctgaa acgtgttcga gttcacggca tcgaacgcga
     2761 gtccgtgctt ggcggcgact tcccgcaagg caacgtagtc actcgtcgcg tcccatggaa
     2821 aatgcggcga tatcgtcggc gttgcccgca ccaaatggtg gatggtggcg caatcctcca
     2881 gcttgtcgaa cagatggcgc ggctcgccgg ggccgggaaa gcgtgcaaag cgggtgccac
     2941 ctgtgccgac gccccaggtc gggatcgcga cgccgaacgc catgaccttt cgtgttagcg
     3001 cgtcaccgtc aatgccccgg cggcccagcg cgcgcaacaa cgcggcgtaa tcctcgtcga
     3061 gcgccggttt tcgcgcggcg ttctcagcct ggagaagatc ctcgctgatg gaaaagtcgg
     3121 tcatcgttgt ctcccgacga ttggcgtctc accgcgtgaa agcctgcggg ttgccggcat
     3181 cgacattgag gatgttgccg gtcgatttgg ccgaaagatc gctggcgaag aaataaaccc
     3241 cctcggcgat gtcctccggc aacaccgaaa gcttcagcat cgatcgctgc cgatagactt
     3301 cttcgagttc gtcttccgaa accttgttgg aggcggcccg ttgctggcgc cattcgccgg
     3361 cccagatttt cgagccgcgc agcaccgcat ccggattgac ggtgttgacg cggataccgt
     3421 gcggcgcgcc ttccagcgca aggcagcgcg ccagatgaat ctccgaggcc ttggccgcgc
     3481 aataggccga cgcgccggcc gaggcggcga ggccgttctt ggaggcgacg aacacgatcg
     3541 cgcctccgca ttgttgccgt tgcatcagcc ggaacgcctc gcgcgccacc agaaaatagc
     3601 cggtggcgag tacatccatg ttcttttgcc aaagtgccag gctggtgtct tcgaccggcg
     3661 aggcggaggc gatgccggca ttggagatca caatatcgat cccgccatag gcgagaatgg
     3721 actcgtggaa gctcgcgaca acgctcgcct cttcggtcac gtccagcggg atgccgaccg
     3781 cgctgtcgcg gccgaatttt tgcacaatcg cttggatgcg cgcgtcgagc gcctcctgat
     3841 cgatgtcggc cagcaccaca caggcgcctt cgcccaatag ccgcaacgcc gttgctccgc
     3901 cgatgccgcc ggcaccgccg gtgacatagg cgatcctgcc ggccagcgat ttcgccttcg
     3961 gcatgcgctg cagcttggcc tcttcgagca gccaatattc gatatcgaag gcttcctgtt
     4021 ccggcagacc gacataggcg ttgacgccgg aggcgccacg catgacgttg atggcgttga
     4081 cgtagaattc cgaagcgatg cgcgccgtcg ccttgtcctt ggcgaaggag aacatgccga
     4141 tgccgggcac caggtagatc accgcattgg ggtcgcgcat cgccagcgaa ttcggccgct
     4201 tgcagcgctg gtaataggcg gaatagtcgt ctcgataggc cccaagaagc gcgtcgagcg
     4261 aattggcgag cgtatcgaca ttgtcgcttt ggcgatcgta gggcagcacc aggggacgga
     4321 ttttggtgcg caggaaatga tcgggacagg atgttcccaa cggcgccagt cgatccagcg
     4381 cgcgcgaatt gacgaactcc agcacttccg gcgcgtcggt gaaatgcccg accttgcgct
     4441 cgtcgcgcga gatcttgccg cggatcagtg gcatcagcgc cgccgcgacg gcgcggcacg
     4501 tctcaggcgc ggcagcatca cagcgcggac cgccgaaggc gggctgcttt tcgtgtcccg
     4561 cgagccagtc ggcggcctgc tggataatgc gcaacgtggt ctggtaggcc gccttcgatg
     4621 tcgggcccca gctgaacaga ccgtggccgc cgagtaccac gccgacatat tcgggatgtt
     4681 gagccgccat cgcgccgagc ttcaggccaa ggtcgaagcc cggccgctgc cagggcagga
     4741 acccgatcgt gccgccgaac acttttcccg tcaattcctc ggcgttggag caggcggcga
     4801 tggcgatcac cgcatcagca tggacatggt cgacatgggg gtgcggcacg aagccgtgca
     4861 aggcggtgtc gatggaggtc gcgcgcgggt tgagattgaa ggtgcagtga ttgaacagcg
     4921 ccaccatctc gtcttcatgg gcgatgccgc gatagagctg tttcaggctt tccaacttgt
     4981 cgagatagag ggtggagaag ccgtcacgct tcatcgagcc gaggtcgccg ccggagcctt
     5041 tgacccacag cacggtgatc ggttcttggg tcagcggatc ggtcattgcg agcttggccg
     5101 aggtattgcc gccgccgaaa ttggtgatgc gaagatcgct gccgagcaga ttggagcggt
     5161 agagcaatag gcccggctca tccaggtccg ccgcatgggc atcgttccaa agatcgggaa
     5221 gcggctcggc cgcagccttg gtcatgaacc tcgcctccgt catgatgcgc ttttgccgga
     5281 ccataggatc gcaaccgatc acggtcaatc acgatgtgag cgatatcgcc gtgcaatatg
     5341 attgatgcag tgcgaaattg acggaatgtg aaggtttcgc gttgaccaac caaaaagttc
     5401 gtgactagtt atgagcgttg ggaggaacag ggccgtgcac gaacgcgagc gctggcaggt
     5461 catcagggcg atgctgaagc agcagctgct ggttcgtatc gccgacgttt gccgcgccac
     5521 cgacgcctcc gaagcttcgg tgcgacgcga ttttgcccgg ctcgccgaaa tgggttcggc
     5581 gacgcgggtg cgcggcggat tggaggcgat gcctggttcg cagatggcct cgagcgacgc
     5641 gccggcgctg gcgacccgct ccttcgagat cagcaagaca ctcaatgtcg ccgccaagcg
     5701 ggcaatcgcc cgggccgcgg ttgcgctgtg cagcgacggc gacgccatca tcatcaatgg
     5761 cggcaccacg accttcgaga tgggcgaatt cctgcgcgag cgccgcctca aggtgctcac
     5821 caattcctat ccattggccg agacgttgat ccacgattcg aac
//
LOCUS       DASWGM010000210         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_431617,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000210 DASWGM010000000
VERSION     DASWGM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431617"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VGA15_06485"
     CDS             <1..804
                     /locus_tag="VGA15_06485"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9587378.1"
                     /translation="RPAEDALANKLRRNLPAKLAYHLIRWRNVLFGMYFFQLSRRKPA
                     RVKILILGGVRMALGPDYDIATHFTPRYNPWDQRLCLVPDGDLFKAIRDKRASVVTNE
                     IDSFTKNGIKLKDGSELEADIIVTATGLNLQVMGGMEVSVDDRAVDFARTLNYKGMMY
                     SDIPNLASAFGYTNASWTLKCDLTCEYVCRLINYMDRHGYRQCMPHNVDPTITELPSL
                     DFSSGYVQRSIAKMPKQGSKRPWRLYQNYALDIVTLRFGAVNDGVMRYS"
     gene            1219..1374
                     /locus_tag="VGA15_06490"
     CDS             1219..1374
                     /locus_tag="VGA15_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587379.1"
                     /translation="MDAQKIAIDAVVALTDCDREQVTAFIRRFYLAGVKDPKRLTFKG
                     LQALARG"
     gene            complement(1407..>1598)
                     /locus_tag="VGA15_06495"
     CDS             complement(1407..>1598)
                     /locus_tag="VGA15_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX9587380.1"
                     /translation="FGNFDPFGDFDLIFGAAKQNLKIIETPIHYKARTFGETQISRFR
                     DGWLLLKMVWFAYRELKAI"
BASE COUNT          317 a          488 c          468 g          325 t
ORIGIN      
        1 cggccggcgg aggatgcgct cgccaataaa ttgcggcgca atctgccggc caaactcgcc
       61 tatcacctga tccgctggcg caacgtgctg ttcggcatgt atttttttca attgagccgg
      121 cgcaagccgg cgcgggtcaa gatcctgatc ctcggcggcg tcaggatggc gctggggccg
      181 gactacgaca tcgccacgca ttttacgccg cgttataatc cctgggatca gcggctgtgc
      241 cttgtccccg atggcgatct gttcaaggcg atccgggata aacgcgcatc cgtggtcacc
      301 aacgagatcg acagcttcac caagaacggc atcaagctga aagacggcag cgagctcgag
      361 gctgacatca tcgtcaccgc caccggtctc aatctgcagg tgatgggcgg catggaggtc
      421 agcgtcgacg accgcgcggt cgattttgcg aggacgctga actacaaggg catgatgtat
      481 tcggatattc ccaacctggc gtccgcgttc ggctacacca atgcatcgtg gacgctgaag
      541 tgcgacctga cctgcgaata tgtctgccgc ctgatcaatt acatggaccg gcacggctac
      601 aggcagtgca tgccgcacaa tgtcgatccc accatcaccg aattgccgtc gctggatttc
      661 tcgtccggtt acgtgcagcg atcgatcgcg aaaatgccga agcagggctc gaagcggccg
      721 tggcggctct atcaaaatta cgcgctcgac atcgtcacgc tgcgcttcgg cgcggtcaac
      781 gacggggtga tgcggtattc gtgagcggcc gcggccgcgt cacatcgacg agccatcgtt
      841 gaacattcct ctcggcaggt ttttcccgac ccgcgcataa tccggcaaat tttgcacctg
      901 tttggtacgc cctcgacccc cggcggcccc ggggcgacgg caccaacccc gagcagatgt
      961 tcgccgcggg ctgccggtag gccacctcga tcagcgaaga gccgggagcg cgttcggctt
     1021 cacgtccggc gcatcaccgc cgccacgttc ggatccgctc cgccggaaaa tttctgcctg
     1081 cgccctaatg tcgctatgcg ttgtttcgcg tattcgatcg ttttcacgaa tcagtcatca
     1141 tgcgaaacgg ttaacgtcct attaatcggc gggtcctacg cttaagtggt tgaggctgct
     1201 gacagcacga ggaagtccat ggacgcccag aaaattgcca tcgatgccgt ggtcgcgttg
     1261 accgattgtg accgcgagca ggtgacggcc ttcatccgca gattttatct cgccggcgtg
     1321 aaagacccca agcgcctgac cttcaagggt ctgcaggcgc tggcgcgggg ctagattgcc
     1381 gctgcggctt tcgacgcctg gccctgtcaa atggctttga gttcgcgata cgcgaaccac
     1441 accatcttca aaagcaacca tccgtcccga aaccgggaaa tctgggtctc gccgaaggtg
     1501 cgcgccttgt agtgaatcgg cgtctcgata atcttgaggt tctgcttggc cgcgccgaag
     1561 atcagatcga aatcgccgaa cggatcgaag ttgccgaa
//
LOCUS       DASWGM010000211         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1261287,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000211 DASWGM010000000
VERSION     DASWGM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261287"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VGA15_06500"
     CDS             complement(<1..282)
                     /locus_tag="VGA15_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587381.1"
                     /translation="MNADVLTTTVADGIAVVTLGSAKRIYFDAEMGDVLTEALEGLAG
                     DPAVGVVIITGGAPGYFNRHFDIPSLIKIAESLRASGREWPENATYNGGF"
     gene            complement(293..>2049)
                     /locus_tag="VGA15_06505"
     CDS             complement(293..>2049)
                     /locus_tag="VGA15_06505"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9587382.1"
                     /translation="PKQLVFGLPTDSAVGNFDAGYDSAPVKIDHQYHTPYCFSQPMEP
                     NACLAVPRGEDLVLYVSTQIVDAARSSVASTLKIDPQRIQVVSTYVGGGFGSKLSVHS
                     ETILAAIAARRLNRPVKVALTRRQIFHDVGVRPTSIQRIRLGAERDGRLVAIAHDATM
                     HTNPQEEFVETSALATRSFYAAPHRLTRHRLVPLDMLRGEDVRAPGEAPGLLALESAM
                     DEVAHALNMDPIELRIRNEPAVDPERNVPYSERRVVECLREGARRFKWERRQQQPGTV
                     RDGRWLVGFGVAAAIRVHPQGPAKARVRLAPDSTCIVLSDMTDIGTGTYTVLAQVAAE
                     RLGLRVDQVRVELGRSDLPASWGSGGSWGASSSAVAVYRACEALREKLQATAQADPHS
                     RPYASGNTATLSDLLVRNHPHGLEAEGDSTPMWADPNFGNYSIHTYGAHFAEVGVDAD
                     TGEIRLRQMLGVFSPGRVLNAKTARSQLIGGMTFGVGMALLEEAVVDPRSGAFVNCDL
                     AGYLVPVHADIPVIDAVILDGFDDKANVLGVKGLGELGICGAGAAVGNAVFNATGVRG
                     RDFPITLDKLLPALPQQV"
BASE COUNT          372 a          684 c          629 g          364 t
ORIGIN      
        1 gaatcccccg ttgtaggtcg cgttctcggg ccactcgcga ccggacgccc ggagggactc
       61 cgcgatctta ataagcgatg ggatatcgaa gtgacgattg aaataacccg gcgcgccgcc
      121 ggtgatgatg accacgccaa ccgcgggatc gcctgccaaa ccttccagag cttcggtcag
      181 aacgtccccc atctcggcgt cgaagtagat gcgctttgcg ctgcccagcg tcacgaccgc
      241 gattccatcg gctacggttg tcgtgagaac gtcggcgttc atcggatcct cgtcagactt
      301 gctgcggcag ggcgggcagc aacttgtcca gggtgattgg gaaatcccgc ccgcgcactc
      361 cggtcgcgtt gaataccgca tttccgacgg cggcccccgc gccgcatatg cccagctcac
      421 cgagcccttt gacgccaagc acattggcct tgtcgtcgaa tccgtcgagt atcacggcat
      481 ctatcactgg aatatccgcg tggaccggta cgaggtaccc ggccagatcg cagttgacga
      541 acgcgccgga ccgaggatcg accacggcct cttcgagcag tgccatcccg actccgaaag
      601 tcatgccgcc aatcagctgc gaacgcgccg ttttggcgtt caggacacgg ccgggcgaaa
      661 agacacccag catctgccgg agccggatct cccccgtatc ggcgtccaca cccacctcgg
      721 caaaatgagc gccgtacgta tggatggagt agttaccgaa gttggggtct gcccacatcg
      781 gggtgctgtc tccctccgct tcgagcccat ggggatgatt gcgtacaagc aagtcggaga
      841 gggtggccgt gttgcccgag gcatagggtc gcgaatgcgg atcggcctgc gccgtagcct
      901 gcaacttttc gcgaagggct tcgcaagcgc gatagaccgc gacagcggag ctgctcgcac
      961 cccacgagcc gcccgatccc cagctcgccg gcagatcgga gcgtcccagt tcgacgcgca
     1021 cctggtcaac tcgcaggccc aagcgctccg ctgccacttg ggcgagaacc gtgtaggtcc
     1081 cggtcccaat atccgtcata tccgacaaca cgatgcaggt gctgtccggc gccagccgca
     1141 cccgcgcttt cgctggcccc tgcgggtgaa cacgaatggc cgcggcaacg ccgaacccga
     1201 caagccatct gccgtcgcgc accgttcctg gctgttgctg acgccgttcc cacttgaacc
     1261 ggcgcgctcc ttcgcgcagg cattcgacaa cgcgccgctc cgagtacggc acatttcgct
     1321 cgggatcgac tgcgggctcg ttccggatgc gcaactcgat gggatccatg ttgagggcat
     1381 gcgcgacctc gtccatcgca gactcaagcg ccagcaggcc cggcgcttcg cccggcgcac
     1441 gcacgtcctc gccgcgcagc atgtctaacg gaaccaggcg atgccgcgtc agccgatggg
     1501 gcgccgcgta gaagctccgc gtggcaagcg cgctcgtttc gacaaattcc tcctgagggt
     1561 tcgtatgcat ggtcgcgtca tgagcgatgg ccacgagccg accgtcccgt tccgcgccca
     1621 ggcggattcg ctggatggac gtgggccgga cgcctacgtc atgaaagatt tgccgccgcg
     1681 tcagcgcgac tttcaccggc cggttcagac gacgcgcggc gatcgcggcc agaatggtct
     1741 cggagtggac gctcagcttg gatccgaaac cgccgccaac ataggtgctc acaacctgga
     1801 ttctctgcgg atcgattttc agcgtgctgg ccaccgaact gcgcgcggcg tcgacgatct
     1861 gggtgctgac gtagagcacg agatcttcgc cgcggggcac ggccaggcac gcgttcggct
     1921 ccatcggttg ggaaaagcaa tacggcgtat ggtactggtg gtcgatcttg accggagcac
     1981 tgtcgtatcc cgcgtcgaaa tttcccaccg cgctatcggt cggcaggccg aagaccaatt
     2041 gcttcggcg
//
LOCUS       DASWGM010000212         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2095830,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000212 DASWGM010000000
VERSION     DASWGM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2095830"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGA15_06510"
     CDS             <1..243
                     /locus_tag="VGA15_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9587383.1"
                     /translation="SVQDTTLGTTVGNLGMEDTKFPRPVFAGDTLRAETKIVAVRESK
                     SRPTQGIVTFEHRGFNQRNEEIAYCRRTGLMMRRPA"
     gene            240..1118
                     /locus_tag="VGA15_06515"
     CDS             240..1118
                     /locus_tag="VGA15_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX9587384.1"
                     /translation="MRLRSLLFVPADSEHKFAKASGIGADALILDLEDSVAPPRKALA
                     RDMVKNLLSGPPRDRAFLVRINPLGSGLTLDDLAAIVRPGLDGILIPKVNGIKDIELI
                     SNYVDVLEIAAGVTPGHVKLLVVATETPAAMIGFASYATPSRRLIAMTWGAEDLGAAL
                     GALSNKEADGSWTFPYQVARAQCLFAAGAAGVMALDTLYADFRDQEGLAESCRIARRD
                     GFVGRVAIHPNQVATINAFFTPSEADLAHARRVVAAFAAQPDAGTLGIDGKMYDMPHL
                     IAAKRALASAGEDVTP"
     gene            1115..>1806
                     /locus_tag="VGA15_06520"
     CDS             1115..>1806
                     /locus_tag="VGA15_06520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9587385.1"
                     /translation="MSSLLVNELEPADDHAAIREGVRAVVNRFGDDYWLARDDDGVFP
                     FEFHRAMAHAGWLGITMPEEYGGSGLGVTEAAIMMHEVASHGGGMSATSAVHINLFGP
                     HPIVVKGTPEQKSRWVPRLVAGEDQCCFGFTEPDAGLNTARIKTFAQKVSGGYVVHGQ
                     KVWTSTAQVANKIMLLTRTTRYEDCARPTDGITIFYTDLDRSKIDVHRIPKMGRKAVD
                     SNAIFIDGLFIP"
BASE COUNT          351 a          528 c          581 g          346 t
ORIGIN      
        1 tccgtgcagg acaccacctt gggaacgaca gtcggaaatc tcggtatgga agacaccaaa
       61 tttccgcgcc cggtctttgc cggcgatacg ctgcgggcgg aaacgaagat tgtggcggtg
      121 cgcgaaagca agtctcgccc aactcaggga atagtcacgt tcgaacatcg tggtttcaat
      181 cagcgcaatg aggaaatcgc atactgccgc cgtaccgggc tgatgatgag gcgtcccgca
      241 tgagattgcg ctcccttctg tttgtgccgg ccgacagcga gcataagttc gcgaaagcat
      301 ccggcatcgg cgccgatgcg ctgattctcg atctcgagga ttccgtcgcg ccgccgcgca
      361 aggcgctggc gcgcgatatg gtgaaaaact tactgagtgg cccgccgcgc gacagggcgt
      421 ttctggtacg catcaatccg ctcggaagcg ggctgacgct cgacgacctc gctgccattg
      481 tccggccagg tctcgacggc atcctgattc cgaaagtcaa cggcatcaag gacatcgaac
      541 tgatttcgaa ttacgtcgat gtgcttgaga tcgccgctgg agttacgccg ggtcacgtga
      601 agctgcttgt ggtcgcgacc gagacgccgg ccgccatgat cggctttgcc agctatgcga
      661 cgccaagccg gcgcctcatc gccatgacgt ggggcgccga ggatctgggc gcagcactcg
      721 gcgcgctgtc caacaaggaa gccgacggaa gctggacgtt cccgtatcag gtagcgcggg
      781 cgcaatgcct gtttgcggcg ggtgccgcgg gcgtcatggc gctggatacg ctatatgcgg
      841 atttcaggga tcaggagggg ctggcggaaa gctgcaggat cgcgcgccgc gatggctttg
      901 tcggacgggt cgccattcac cccaatcagg tcgcgacgat caatgcattc ttcaccccgt
      961 cggaagcgga tctggcgcat gcgcgtcgtg tggtggcggc cttcgcggct cagcccgacg
     1021 ctggaacgct tgggatcgac ggcaagatgt acgacatgcc gcatctgatc gcggcgaaac
     1081 gcgcgctcgc ctcagccggg gaggacgtca caccatgagt tcactgttgg tcaatgagtt
     1141 ggagccggcg gacgatcacg cggccattcg agaaggcgtg cgcgcggtgg taaaccgctt
     1201 tggcgatgat tactggctgg cgcgcgacga cgacggcgtg tttccgttcg agtttcatcg
     1261 tgcgatggcc catgccggct ggctcggcat cacgatgccg gaagaatatg gcggttccgg
     1321 tcttggagtg accgaggccg cgatcatgat gcatgaagtc gcgagccatg gcggaggaat
     1381 gtccgctacc tcagcggtgc acatcaacct gttcggacct catccgatcg tcgtgaaggg
     1441 cacgccggag cagaaatccc gctgggttcc gcggctggtc gcaggcgaag atcaatgctg
     1501 cttcggtttc accgagccgg atgctgggtt aaatacggcc cgcatcaaga ccttcgccca
     1561 gaaggtgtcc ggcggctatg tcgtgcatgg ccaaaaggtc tggacctcga ccgcgcaggt
     1621 cgcaaacaag atcatgctgc tgacccggac cacccgctac gaggactgcg cgcggccgac
     1681 ggacggcatc acgatcttct ataccgatct cgatcgttcg aagatcgatg ttcatcgaat
     1741 tccgaaaatg ggccgcaagg cggtcgattc gaatgccatc ttcatcgacg gcctcttcat
     1801 tccgga
//
LOCUS       DASWGM010000213         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1956418,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000213 DASWGM010000000
VERSION     DASWGM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956418"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..244
                     /locus_tag="VGA15_06525"
     CDS             29..244
                     /locus_tag="VGA15_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX9587386.1"
                     /translation="MSDMKALSERIDVLETRLAFQDETIETLNKTITAQWQQIDALTR
                     HVAVLSERLQEAETHARGAANEPPPHY"
     gene            204..617
                     /locus_tag="VGA15_06530"
     CDS             204..617
                     /locus_tag="VGA15_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587387.1"
                     /translation="MRVALPTNRRRIIEDREAKIMLFKRRDTSQRRIPRPQKRTAFAR
                     KAARSARMRSIAGSTFDDHRLVVAIMIPVVIAILSDHDGIVAIPAVTLTNNLTVAVPI
                     TITMAISDGHADRADTNSWSKSRFIQTAFSSAAID"
     gene            complement(639..860)
                     /locus_tag="VGA15_06535"
     CDS             complement(639..860)
                     /locus_tag="VGA15_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587388.1"
                     /translation="MAEKFDPAAHDRHAVNPREAAAADRDTHAKLEAGLIDSFPASDP
                     VSAAQPAPSKADGDRAAASLWAKVRAVFR"
     gene            1350..2387
                     /gene="hemE"
                     /locus_tag="VGA15_06540"
     CDS             1350..2387
                     /gene="hemE"
                     /locus_tag="VGA15_06540"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX9587389.1"
                     /translation="MHAPKPFLDVLSGRRQPAPPIWMMRQAGRYLPEYRELRARAGGF
                     LDLCFTPEYAAEVTLQPIRRFNFDAAIIFSDILVIPYALGRSVRFEVGEGPRLDPLET
                     PEKVGTLAQHGDFSKLEPVYEALRRVRRELDPNIALIGFCGAPWTVATYMVAGQGTPD
                     QAPARMMAYRHPEAFSKIIDVLVENSIEYLLGQLKAGADVLQIFDTWAGVLPPREFTR
                     WSTEPTRRIVEGVRAKMPDAKIIGFPRGAGALLPGYVDATGVDAVSIDWAAEPSLIRE
                     RVQSRVAVQGNLDPLVLIAGGAALDRAIDDVLENYAGGRLIFNLGHGIQPETPIAHVE
                     QMIKRVRAYRG"
     gene            complement(2608..>3087)
                     /locus_tag="VGA15_06545"
     CDS             complement(2608..>3087)
                     /locus_tag="VGA15_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX9587390.1"
                     /translation="MQRLPDGGAVGPADALIMARSGLERVGLAGRIAYEFSIREMLPA
                     VGQGIVAVECAIDDWQTRRMLALIDDPAAHRSADAEREVLWVLNGHCNSPIAGFSTID
                     GAQMSLTASVLDEAGGLFIEASRSGPANRPRELGRAVGFDLLAKGAAEIIERSRPKL"
BASE COUNT          591 a          974 c          962 g          560 t
ORIGIN      
        1 cgggagcgcc tgaccgaaca ggattccgat gagtgacatg aaggcgctca gcgagcgcat
       61 cgatgtactc gaaacgcgac tggcgtttca ggacgaaacc atcgagacgc tgaacaagac
      121 catcaccgcg cagtggcaac agatcgacgc gctgacgcgg catgtcgccg ttttaagcga
      181 gcgcctgcag gaagccgaga ctcatgcgcg tggcgctgcc aacgaaccgc cgccgcatta
      241 ttgaagatcg cgaagcgaaa atcatgcttt tcaagcgtag agacacgtcg cagcgccgca
      301 taccacgccc acaaaagaga acggccttcg caaggaaggc cgctcgatcg gctcgaatgc
      361 gatccatcgc gggatcaacg ttcgatgatc accgtcttgt ggtcgcgatc atgatcccgg
      421 tcgtgatcgc gatcctttct gatcacgacg gtatcgtcgc gatccctgcg gtcacgctca
      481 cgaacaacct cacggtcgcg gtcccgataa ccatcaccat ggccatttct gacggtcacg
      541 ccgaccgggc cgacaccaac tcttggtcaa aatcccgatt cattcagacg gccttcagca
      601 gcgcagcgat tgattaaatg aaatcgaact ctgcaaatct atcggaacac cgctcggacc
      661 ttggcccaaa gcgaggccgc tgcgcgatcg ccatcggcct tcgatggtgc cggttgtgcg
      721 gcgctgaccg gatcggatgc cggaaaactg tcgatcagac cggcctccag tttcgcgtgg
      781 gtgtcgcgat ccgccgctgc ggcttcgcgc ggattgacgg catgcctgtc gtgcgccgcc
      841 ggatcgaatt tctcagccat gttttgtctc catcgcaagc taacgcctcg gcactccgga
      901 cgttccaaac aacaggagcg gaacatcatc aggggtgttc gaatagaccc ggtcgcgcgc
      961 acgaatttgg cgctgcaatg cccggtaacg ggcacaggat cggatttttt tcgctaaatg
     1021 ttcgatatgg tacagacaag gtggcgcaac ttggggtcga ttagttaccg atgagattgc
     1081 accctcatcg agcttgcgat gctccaaaga caaaattccg atcccagccc gtttggaatt
     1141 tcgcatttgg aacgcccggc agacccgatc gccaggctgg aggactgtgc gggaaggtct
     1201 tgaacgtcgg gacgacgtga tgtttcccgc gagcgatcgc cgcgatcgtt cgggctggga
     1261 caatcgcagc ttggccaaat ccatatccgc agtgtatcag gagcggcaac gcccctctcg
     1321 atcaccctgc ggaacagcat ttgacccaga ttcatgcccc aaaacccttc cttgacgtgc
     1381 tttccggccg caggcagccg gcacctccga tctggatgat gcggcaggcc gggcgctatc
     1441 tgccggaata tcgcgaattg cgcgcgaggg cgggcggctt tctcgatctg tgcttcacgc
     1501 cggaatatgc cgccgaggtg acgctgcagc cgatccgccg cttcaacttc gatgccgcga
     1561 tcatcttctc ggatattctt gtgatcccct acgcgctggg tcgttctgtc aggttcgagg
     1621 tcggcgaagg gccgcggctc gatcccctgg agacgccgga gaaagtcggc acgctggcgc
     1681 agcacggcga cttcagcaaa ctcgagccgg tctatgaggc gctgcgccgg gtacgccgcg
     1741 agctcgatcc gaacatcgcg ctgatcggct tttgcggcgc gccgtggacg gtggcgacct
     1801 atatggtggc ggggcaaggc accccggacc aggcgccggc gcggatgatg gcctaccgcc
     1861 atccggaagc gttttccaag atcatcgacg tgctggtgga gaactcgatc gagtatctgc
     1921 tgggacagct caaggccggc gccgacgtgc tgcagatctt cgacacctgg gccggcgtgt
     1981 tgccgccgcg cgaatttacg cgctggtcga ccgagccgac gcggcgcatc gtcgaaggcg
     2041 tacgtgcgaa aatgccggac gcgaaaatca tcggctttcc cagaggcgcc ggcgcgctgc
     2101 tgccgggtta tgtcgacgcc accggcgtcg atgccgtcag catcgactgg gccgccgagc
     2161 cgtcactgat ccgcgagcgc gtgcagagcc gcgttgccgt tcagggcaat ctcgatccgc
     2221 tggtgctgat cgccggaggt gctgcgctgg atcgcgccat cgacgacgtg ctggaaaatt
     2281 acgccggcgg cagattgatc ttcaacctcg gtcacggcat ccagccggaa accccgatcg
     2341 cgcatgtcga gcagatgatc aagcgggtgc gggcgtatcg ggggtgagga tcaagcgaat
     2401 gcgtactctt ccctctcccc ttgtgggaga gggtgcccag gcggactacg gccgccgttc
     2461 ttggatagaa cgccgatgct ttgcatcggc tatggcgaag cgaaggcggg tgagggggtg
     2521 atgagcctcg ttgtgtggcg ccccctcacc cggcacggac ttcgtccgcg ccaccctctc
     2581 ccacaagggg agagggaaga gaaagcatca cagcttgggc cggctgcgtt cgatgatctc
     2641 ggccgcgccc ttggcaagca ggtcgaaccc gaccgcacgg ccgagttcgc gcgggcggtt
     2701 ggcggggccg gagcgcgagg cttcgatgaa cagtccgccc gcttcatcga gcaccgaggc
     2761 ggtcagcgac atctgcgccc cgtcgatggt tgaaaatccc gcgatcggcg agttgcagtg
     2821 gccgttgaga acccacagca cctcgcgctc ggcatcggcg cttcggtgcg cggcgggatc
     2881 gtcgatcagc gccagcatgc gccgggtctg ccagtcatcg atcgcacact ccaccgcgac
     2941 gatcccctgc cccaccgccg gcagcatttc ccgtatcgag aattcatagg cgatacgacc
     3001 ggcgaggccg acgcgttcca gccccgagcg cgccatgatc agcgcgtccg ccggaccgac
     3061 cgcgccgcca tccggcagcc gctgcat
//
LOCUS       DASWGM010000214         3393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_19420,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000214 DASWGM010000000
VERSION     DASWGM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19420"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..350)
                     /locus_tag="VGA15_06550"
     CDS             complement(45..350)
                     /locus_tag="VGA15_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587391.1"
                     /translation="MTQLLKITALSAILAVGIVAASAVQAEQNQPSPMGNGMQQRGMM
                     GGEMGRMMGQDHQGGTDTDDTMMDTRQMNQMMSHCNQMREGRMQAPNSQFPKPEHKG"
     gene            complement(383..721)
                     /locus_tag="VGA15_06555"
     CDS             complement(383..721)
                     /locus_tag="VGA15_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587392.1"
                     /translation="MHTAEVVKAPTAEGARRRSLADRLPRWLIGWRGLVILGILLVVA
                     GLYAGWGWLAAVGLAPIILSLAPCAVMCAIGACAMSRGGSSGATQATPNTADPAGPSA
                     ADRFSNARND"
     gene            complement(780..1520)
                     /locus_tag="VGA15_06560"
     CDS             complement(780..1520)
                     /locus_tag="VGA15_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX9587393.1"
                     /translation="MTELSLVAVVTSFAAGVISFLSPCVLPLVPGYVSYVAGHSLANT
                     TPGTEVVARFGALRSSLFFVFGFSTVFIILGASATALGQSLLSYRYEANIAAGVLVIV
                     FGLFMMGLFRLPWLQRDFRFHFQLPGGEPTAAYLIGIAFAFGWTPCIGPVLGAILTTS
                     AVSATVPQGIALLFVYSLGLGVPFLAAAIFTEWLLVQLRLLARIGRFLQIGAGGLLVA
                     MGAAMMTGELSAFSYWLLETFPVFARIG"
     gene            complement(1556..2491)
                     /locus_tag="VGA15_06565"
     CDS             complement(1556..2491)
                     /locus_tag="VGA15_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX9587394.1"
                     /translation="MPSARLGKRNKRGSIWGAAVLFSAVVITGAALGFGSWSQTPSRM
                     PLSTLLSSTHVHGIAVDPKEPQRLYLATHTGFFAVGRDGVATRLSDNQNDYMGFTPHP
                     ADPTVFFASGHPSTGGNLGFMTSTDGGKSWQQLAKGASGPVDFHQMDVSKADPAVIYG
                     VYSGLQVSRDGGRSWTMTGPAPAGIIDLAASAKDVNTLYAATQTGLLVSHAGGKSWQP
                     SNLGEQPVSMVKTTSAGDVYAFVVGTGLVHATEPSLTWNTLGNGFRGAYVLHLAVDPS
                     SKDRLYAATFDPATHRGDVLATADAGRTWSPLGAR"
     gene            complement(2524..>3393)
                     /locus_tag="VGA15_06570"
     CDS             complement(2524..>3393)
                     /locus_tag="VGA15_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9587395.1"
                     /translation="GIRSLLAVTALLMLSSFSAMPARAHSPQQLETQLQRQEKFFQPV
                     GRLAPEFDLQDSEGHPWDLDRLRGKVVVLNFIYAGCKDLCPLHSELIAELQKMINLTP
                     MRDQVQFVSITTDPGRDGSDVLKAYGAAHGLESSNWTFLTTRPGQPEDATRRLAERFG
                     HKFTRTDDGEYAHGIVTHVIDRDGMMRGNFHTLKFEPTNLVMFVNALANDVHKPGHAE
                     DGAAGIEPSGRDAHAAASGWISLVPVALLVLAAGWLMGAITFFGLRRMRSRANARRSG
                     SDVSITDTRRTGR"
BASE COUNT          642 a         1051 c         1107 g          593 t
ORIGIN      
        1 gcatgagggg tgaagccgag ccaaatggcg acgcttccgg cgcgtcagcc cttatgctcc
       61 ggctttggga attggctgtt cggcgcctgc atgcggcctt ccctcatctg attgcaatgc
      121 gacatcatct gattcatctg ccgcgtatcc atcatggtgt cgtcggtgtc ggttccgccc
      181 tgatgatcct ggcccatcat gcggcccatc tcgcctccca tcatgccacg ctgctgcatg
      241 ccattgccca tcggcgacgg ttgattctgc tccgcctgaa ctgctgatgc ggcgacgatg
      301 ccgacagcga gtatggcgct cagcgcggtg atcttgagca attgtgtcat cgggtatcct
      361 ttctacggtg cgagttgtag gactagtcgt tgcgcgcgtt tgagaaccga tcggctgcgg
      421 acggcccagc tggatcggcg gtgttcggcg tcgcctgagt cgcgccgctc gatccgccgc
      481 gcgacattgc gcaggcacct atggcgcaca tgacggcgca gggcgcgagc gacaggatga
      541 tcggcgcaag gccgacggcg gcgagccagc cccagccggc atagagaccg gctacaacca
      601 agagaatgcc cagaatgacg agtccgcgcc agccgatcag ccagcgggga agccgatccg
      661 ctaatgaccg gcgccgtgcg ccttcagcgg tcggtgcctt cacgacttcc gcggtatgca
      721 tggttcgctc tcctttctag tgagatggtg tttccatatg gtggaaggtc aacgggcgtt
      781 catccgattc tcgcgaagac cgggaaggtc tcgagcagcc agtaagagaa ggcggagagc
      841 tcgccggtca tcatcgcagc gcccatggcg acgagcagcc cgccggcgcc gatctgcaga
      901 aagcggccga tgcgtgcgag cagccgcagc tggacgagaa gccactcggt gaatatcgcg
      961 gccgcaagaa acgggacacc gagccccagg gagtagacga aaagcagagc gatgccttgc
     1021 ggcacggtcg ccgagaccgc gctggtcgtc aggatggcac cgagcaccgg accgatgcac
     1081 ggcgtccatc cgaaggcgaa ggcgatcccg atgagataag cggctgtcgg ctcgccgccg
     1141 gggagctgga agtggaagcg gaagtcgcgt tgcagccaag gcaagcggaa cagacccatc
     1201 atgaacaggc cgaagacgat gacgaggacg ccagccgcga tattggcttc gtagcgatag
     1261 gacagcagcg attgcccgag cgccgtggcg ctggcgccca agatgatgaa tacggtcgag
     1321 aagccgaaga cgaagaagag gctcgaacgc aacgccccga atcgcgcgac gacctccgtg
     1381 cccggcgtcg tattcgctaa agagtgcccg gcgacatacg acacataacc cgggaccaga
     1441 ggcagcacgc aaggtgacag gaaagagatc acgcctgcgg cgaagctcgt caccacggca
     1501 accaacgaaa gctccgtcat cgcgcgccac ccctgcccgc ttcgatcgac gccattcacc
     1561 gcgctccgag cggcgaccag gtgcggccgg catccgcggt ggccagcacg tctccccggt
     1621 gtgtcgcggg gtcaaacgtc gccgcgtaga ggcgatcctt gctggacgga tcgaccgcaa
     1681 gatgcaggac ataggcaccc cgaaaaccgt ttccgagggt gttccaggtc aggcttggct
     1741 ccgtcgcatg gaccaggccg gtgccaacga cgaaagcgta gacatcgccg gccgacgttg
     1801 tctttaccat cgagacaggt tgctccccga gattcgatgg ctgccagctc ttgccgccgg
     1861 catggctgac aagcagtccg gtctgggtgg cggcatagag cgtatttacg tctttggcgg
     1921 aggcggcgag atcgatgatg cccgccggcg cagggccggt catcgtccag ctgcggccgc
     1981 catcccggct cacttgcagc ccgctgtaca cgccatagat caccgccgga tcggccttgc
     2041 tgacgtccat ctggtggaag tcgaccgggc ccgaggcgcc cttcgccagc tgctgccatg
     2101 acttgccgcc gtctgtcgat gtcatgaacc cgagattgcc gccggtggaa ggatggccgc
     2161 tggcaaagaa caccgtcggg tcggccggat gcggcgtgaa gcccatatag tcgttctggt
     2221 tgtcggacag ccgtgtcgcc acgccatcgc gtcctaccgc gaagaagccg gtatgggtcg
     2281 cgaggtagag ccgctgcggc tcctttgggt cgactgcgat accgtggacg tgcgtcgacg
     2341 acaatagcgt cgatagaggc atacggctcg gcgtctgcga ccacgatcca aacccgagcg
     2401 cagcgccggt gatgaccacg gccgagaata ggacggccgc tccccagata gaccctcgct
     2461 tgttgcgctt gccgaggcgc gcgctcggca cgacggctct cgtcttgttt gctgctcgcg
     2521 tcgtcatcgc cctgtccttc gtgtgtcagt gatgctcacg tccgacccag atcgacgtgc
     2581 gttggcgcgc gagcgcattc gacggagacc gaaaaacgtg atggcgccca tcagccatcc
     2641 tgcggccagg acgagaagcg ccaccggaac cagcgagatc caccccgaag cggcggcatg
     2701 tgcatcacgg ccggacggct cgatgccggc ggcaccatcc tccgcgtggc cgggcttgtg
     2761 cacgtcgttg gcgagcgcat tgacgaacat caccaggttg gtcggttcga acttcagcgt
     2821 atggaaattg ccgcgcatca tgccgtcgcg atcgatcaca tgcgtcacaa tgccgtgggc
     2881 gtattcgccg tcatccgtgc gcgtgaattt gtgtccgaaa cgctcggcga gccggcgggt
     2941 tgcgtcctcc ggctggccgg gacgtgtcgt gaggaaggtc caattgctgg attcgaggcc
     3001 atgagccgcg ccataagctt tgaggacatc tgagccatcg cgccctggat cggtggtgat
     3061 cgagacgaac tgcacctggt ctcgcatcgg cgtcaggttg atcattttct gcagctcggc
     3121 gatgagctcg ctgtgcagcg ggcaaaggtc cttgcagccg gcatagatga agttcagcac
     3181 gacgaccttg cctctcagtc tgtcgagatc ccatggatgg ccctcgctgt cctggaggtc
     3241 gaactcgggt gcgagacggc ccacgggttg gaaaaacttc tcttggcgct gaagctgcgt
     3301 ctcgagctgt tgcggcgagt gggcgcgggc cggcatcgct gaaaacgacg aaagcatcag
     3361 cagcgcggtt acggcgagca atgatcgtat gcc
//
LOCUS       DASWGM010000215         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2513244,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000215 DASWGM010000000
VERSION     DASWGM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513244"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2424
                     /locus_tag="VGA15_06575"
     CDS             <1..2424
                     /locus_tag="VGA15_06575"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX9587396.1"
                     /translation="AIGKDAGMATKHDWYKAAALALRDRIVHHWLTGEKQSYDAGSKR
                     VYYLSLEFLIGRLFTDALNNMGLLPVFETALGDLGVSLAELRKCEPDAALGNGGLGRL
                     AACFMESMATLAIPAIGYGIRYDFGLFRQIIANGWQHEYPDEWLGFGNPWEFQRPEVV
                     YHVHFGGSVEHVEEKGRDRAVWHPAETVQAVAYDTPIVGWRGQHVNALRLWSARSPDP
                     LKLDVFNTGDYLGASAEEARAESICKFLYPNDESPAGRELRLRQEYFFVSASLQDLIK
                     RHLVSDGQLRGLASKAAVQLNDTHPSLAVTELMRILCDLHNFRWDEAWKITVATLSYT
                     NHTLLPEALETWPVELFERLLPRHLEIIYRINVAHLALADARCPGDDDFKASVSLIDE
                     RSGRRVRMGQLAFVGSHRINGVSAMHSDLMKETVFHDLNHLYPARITNKTNGITFRRW
                     LMLANPKLTDLLREACGEAVLDDPSLLERLEARASDNAFQQQFRAVKRHNKLALARLI
                     GERLNVVVDPGALFDVQIKRIHEYKRQLLNILETVALYHAIKDEPQRDWVPRVKIFAG
                     KAAASYRYAKLIIKLINDVAEVVNNDPEIAGRLKVAFLADYNVSLAEVIIPAADLSEQ
                     ISTAGMEASGTGNMKLALNGALTIGTLDGANIEIRDHVGAENIAIFGMEALDVVVRRK
                     QGLDATDVIRRSPHLSRAIKSIESGEFSPDDPARFESIGHALRHLDHYMVSTDFDAYF
                     EAQRGIDVRWQVVPAWTRASILNVARMPWFSSDRTIREYARDIWNVPVRGPSPQGLQE
                     PGAQRGATR"
BASE COUNT          516 a          856 c          818 g          466 t
ORIGIN      
        1 gcgatcggca aggatgccgg catggccacc aagcacgact ggtacaaggc ggcggcgctg
       61 gcgctgcgcg accgcatcgt gcaccattgg ctgaccgggg aaaagcagag ctacgacgcc
      121 ggcagcaagc gggtttatta cctcagtctg gaattcttga tcggccgcct gttcaccgat
      181 gcgctgaaca atatggggct attgccggtg ttcgagaccg cgctgggcga tctcggcgtc
      241 agtctggcgg agttgcgtaa atgcgagccg gacgcagccc ttggcaatgg cggtcttgga
      301 cggcttgcgg cgtgcttcat ggagagcatg gcgacgctgg ccattcctgc catcggctat
      361 ggcatccgct acgatttcgg cctgttccgc cagatcatcg cgaatggctg gcagcacgaa
      421 tatcccgacg aatggctggg cttcggcaac ccgtgggaat tccagcggcc cgaggtggtc
      481 taccacgtgc atttcggcgg cagcgtcgaa cacgtcgaag agaaaggccg cgaccgcgcg
      541 gtctggcatc cagcggaaac cgtgcaggcc gtcgcctatg acaccccgat cgtgggctgg
      601 cgcggacaac acgtcaatgc gctgcggctg tggtcggcgc ggtcgcccga cccactcaag
      661 ctcgacgtgt tcaataccgg cgactatctc ggcgccagcg cggaagaagc gcgcgccgaa
      721 tcgatctgca agttcctcta cccgaatgac gagagcccgg cgggccgcga gctgcggctg
      781 cgtcaggaat atttctttgt ctcggcctcg ctgcaggatc tgatcaaacg ccacctcgta
      841 tccgacggcc agctgcgcgg gctggcgtca aaggcggccg tgcagctcaa cgacacccat
      901 cccagcctcg ccgtcaccga gctgatgcgc attctctgcg atctccacaa cttccgctgg
      961 gacgaggcgt ggaagatcac ggtggcgacg ctgtcctaca ccaaccacac cctgctgccg
     1021 gaagcgctcg agacctggcc ggtcgaactg ttcgaacgcc tgttgccgcg acatctggag
     1081 atcatctacc gcatcaatgt cgcgcatctt gcgctggccg atgcgcgctg tcccggcgat
     1141 gacgacttca aggcctcggt atccctgatc gacgaaagat ccggccgcag ggtacggatg
     1201 ggacaattgg cgttcgtcgg atcgcaccgc atcaacggcg tctcggcgat gcattccgat
     1261 ctgatgaagg agaccgtgtt ccacgatctc aatcaccttt atcccgcccg catcaccaac
     1321 aagaccaacg gcattacttt ccgccgctgg ctgatgctgg ccaacccaaa gctgaccgat
     1381 ctgttgcgcg aggcctgcgg cgaagccgtg ctcgacgatc ccagcctgct tgaacgtctc
     1441 gaagcccgcg ccagcgacaa tgcgttccag cagcaattcc gcgccgtcaa gcgccacaac
     1501 aagcttgcac tggcacggct gatcggcgag cgcctcaacg tcgtggtcga tcccggcgcg
     1561 ctgttcgacg tccagatcaa gcgcatccac gaatacaagc ggcaactgct caacatcctt
     1621 gaaaccgtag ccctgtatca cgcgatcaag gacgagccgc agcgcgactg ggtgccgcgc
     1681 gtgaaaatct ttgccggcaa ggcggcggcg agctatcgct acgccaagct gatcatcaag
     1741 ctgatcaacg acgtcgccga agtcgtcaac aacgatcccg agatcgccgg ccgcctgaag
     1801 gtcgcgtttc tggccgacta caatgtcagc ctcgccgagg tgatcattcc cgccgccgac
     1861 ctctcggaac agatttcgac ggcgggcatg gaagcctccg gcaccggcaa catgaagctc
     1921 gcactgaacg gcgcgctgac catcggcacg ctcgacggcg ccaatatcga aatccgcgat
     1981 cacgtcggcg ccgagaatat cgcgatcttc ggcatggagg cgctcgatgt ggtggtgcgg
     2041 cgcaagcagg ggctggacgc caccgacgtg atcaggcggt cgccgcatct ttcgcgcgcg
     2101 atcaaatcga tcgagagcgg cgaattctcg cccgacgatc cggcccggtt cgaatcgatc
     2161 gggcacgcgt tgcgccatct cgaccactac atggtcagca cggatttcga cgcctatttc
     2221 gaggcccagc gcggcatcga cgtccgctgg caggtggtgc cggcatggac gcgggcctct
     2281 atcctcaacg tcgcgcggat gccgtggttc tcctcggacc gcaccatccg cgaatacgcc
     2341 agggatatct ggaacgtgcc ggtgcgcggg ccttcgccgc aggggctgca ggagccgggc
     2401 gcgcagcgtg gggcaacccg ctgatcccgt gaagcggaaa tacactacct ctgacgtcat
     2461 tgaattcggc ggaatagccg cgatgctgct cggaaaggca agagcgtctt tgcgaggatc
     2521 gggatagggg ggaacctcac ggctcctccc ctcccacacc accgtacgta cgggtccgta
     2581 tacggcggtt cgacagatta agcacggtcg gtgtttcacg ccgtgccgga gagtcagaca
     2641 gtggagtccg tcgcaa
//
LOCUS       DASWGM010000216         4424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1819586,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000216 DASWGM010000000
VERSION     DASWGM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819586"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGA15_06580"
     CDS             complement(<1..225)
                     /locus_tag="VGA15_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HEX9587397.1"
                     /translation="MQLLNKDDGAAASIDQPGLLAPDTSGVNFYRADPALTDLLRIHL
                     PDALFRHIEPHLDRLGALAGGHLDECARLAD"
     gene            318..1316
                     /locus_tag="VGA15_06585"
     CDS             318..1316
                     /locus_tag="VGA15_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9587398.1"
                     /translation="MADGYRIIGAEMSPYSVKVRAYFRYKAIPHQWILRNAASQAEYE
                     KYAKMPIIPLVVTPEGAGIQDSTPIIELVEKLYPEPPTNPDEAVTHFISALIEEFGDE
                     WANKWMFHYRWARDVDQRSSAGRIARMRSPRASEAEHDAFANQVRARMVDRVWFVGSN
                     AVTAPQIEAGFVEMLGLLDAHLATRPYLFGGRPAFGDFGLWGQFYELWTDPTAGALIE
                     GGAPNVLDWVHRMLWPRAEGQFEAWSALEPTLMPILTRQVGGQFMPWTCANEKALTEK
                     QEEFCVALGDKVWIQKPQKYHSRSLAMLRTKYADVADKSALDPVLESAGCLNGLRG"
     gene            1350..2051
                     /locus_tag="VGA15_06590"
     CDS             1350..2051
                     /locus_tag="VGA15_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9587399.1"
                     /translation="MEKPKYKTALVVGAGEGLSASLARLFAREGLRVALAARRIEKLG
                     ALCSETGARAFACDATNPDEVERLFGLVEREIGTPDVVVYNASGRARGAFVDLVPADV
                     AQAIAVSAFGGFLVAQQAAKRMLPDKHGAILFTGASASVKGYSQSAPFAMGKFALRGL
                     AQSMARELSPQGIHVAHFVIDGGIRSAARTEPEDRPDSMLDPDAIAFSYWNVLQQPRS
                     AWTWELELRPWVEKF"
     gene            2271..3104
                     /locus_tag="VGA15_06595"
     CDS             2271..3104
                     /locus_tag="VGA15_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9587400.1"
                     /translation="MTTTETTIDTGTSELKCVIRDRVAIVTLNRPEVRNALSDNLTPA
                     LRTIIKACGESPDVGALLITGAGTAFCAGGNVKGMGAHRDKKKLAMSYDEKVADLQER
                     QRLLTGALVSVRKPTIAALPGPAVGAGLAIAMACDIRIAAESAFISTGYLRMGLSGDY
                     GIAWLLTRLVGTSRARELMFTADKVDAARCEAIGLVNRVVPDAKLQDAAFALAKSMAE
                     GPTQALRYMKDNLDEALSFDFATARDQEAERLIRLTTTADHREAVQAFIEKRKAVFRG
                     S"
     gene            complement(3109..3723)
                     /locus_tag="VGA15_06600"
     CDS             complement(3109..3723)
                     /locus_tag="VGA15_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9587401.1"
                     /translation="MIALRLNDLRQHSDVLRSRSGKLLTVRFVEPRDADALQGYFRSL
                     SVGSRYNRFLGAMSELPHSELDHFTHVGEAGRFSVIALMTIDGIETIVGEARYAFDAE
                     TLSFEFGLSIDDRWQGDGIGSALLKNLQCRAATFGAERLFGDTLRSNDVMISLARKSG
                     FAFTNSPGDWKLVRFEKHIDAAPQEISCASWRLAAQQIALQAAV"
     gene            complement(3797..4000)
                     /locus_tag="VGA15_06605"
     CDS             complement(3797..4000)
                     /locus_tag="VGA15_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX9587402.1"
                     /translation="MSTCTHESMINRHGSGVFAQIGETLRLWRERAHERRRLAELTDR
                     DLHDVGLSRADIFFEAEKPFWRA"
     gene            4116..>4424
                     /locus_tag="VGA15_06610"
     CDS             4116..>4424
                     /locus_tag="VGA15_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9587403.1"
                     /translation="MTARLPSLNGLRAFEAAARHLSFTHAASELNVTQTAISHQIRRL
                     EQELGIRLFVRQNRALALTPEARDYLPGVRAAFNDLRLATDRLLRRDNDHVLTVSTLA
                     S"
BASE COUNT          891 a         1351 c         1389 g          793 t
ORIGIN      
        1 atcggccagc cgcgcgcatt cgtcaagatg accgccggcg agcgcgccca gccggtcgag
       61 atgcggctcg atatggcgaa acagcgcatc gggcagatga atgcgaagca gatccgtcag
      121 cgccggatcg gcccggtaaa aattcacgcc ggacgtatcc ggcgccagca accccggctg
      181 atcgatcgag gccgctgcac catcgtcctt attgagtagc tgcattttac aactcttgtt
      241 cgttccgctc agtattgaat gatatagatc attctccaga taaagcgggc cgtcgagaca
      301 aaagcggagg accacccatg gccgacggct atcgcatcat cggcgccgag atgtcgccct
      361 actcggttaa ggtgcgcgcc tattttcgct acaaagccat cccgcatcag tggattttgc
      421 gcaatgcggc aagccaggcg gaatacgaga aatacgccaa aatgccgatc atccccctcg
      481 tggttacgcc ggagggcgct ggcattcaag attccacgcc gatcatcgag ctagtcgaga
      541 agctttatcc ggagccgccg accaaccctg atgaggcagt cacgcacttc atttccgcat
      601 tgatcgagga attcggcgac gaatgggcca acaaatggat gttccactac cgctgggccc
      661 gcgacgtcga ccagcgtagt tcggccggcc gcatcgcgcg catgcgcagc ccaagggcca
      721 gcgaggcgga gcatgatgca ttcgcaaacc aggtgcgcgc ccgcatggtg gatcgggtct
      781 ggttcgtcgg ctcgaatgcg gtcaccgcgc cgcagatcga ggcgggtttc gtcgagatgc
      841 tgggcctgct ggacgcgcat ctcgcaacga ggccctattt gttcggcggg cgaccggcgt
      901 ttggcgattt cgggctctgg ggccaattct acgagctatg gaccgatccg accgcaggcg
      961 ccttgatcga gggcggcgcg ccgaatgtgc tggattgggt gcatcgcatg ctctggccaa
     1021 gggccgaggg ccagtttgag gcgtggtccg cgctggagcc gaccctgatg ccgatcctca
     1081 cccgacaggt cggaggacaa ttcatgccgt ggacatgcgc caacgagaaa gcgcttaccg
     1141 agaagcaaga ggaattctgc gtcgcgctcg gcgacaaggt ctggatccag aagccgcaaa
     1201 aatatcactc gcggtcgctc gccatgctgc gcaccaaata tgccgatgtt gccgacaagt
     1261 ccgcgctcga tcctgttctg gagagcgcag gttgccttaa cggattgcgc ggctgaaaac
     1321 gcaactttaa aggataagga aaaacgctca tggaaaagcc aaagtacaaa accgccctcg
     1381 ttgtcggcgc cggcgagggc ctcagcgcat cgctggcgcg gctgttcgcc cgcgaaggcc
     1441 ttcgggtcgc gctggcggcg cgaaggatcg aaaaactggg cgcgctctgc agcgagaccg
     1501 gcgcacgcgc ctttgcctgc gatgctacca atcccgacga ggtcgagcgc ctgttcggcc
     1561 tggtcgagcg cgagatcggg acgcccgatg tggtcgtcta caacgccagc ggcagggcgc
     1621 gcggtgcctt cgtcgatctg gtgccggccg atgtcgcgca ggcgatcgcg gtcagcgcct
     1681 tcggtggctt tctggtggcg cagcaggcgg caaaacggat gctgcccgac aagcacggcg
     1741 ctatcctgtt caccggcgct tccgccagcg tcaagggcta ttcgcaatcc gcacccttcg
     1801 cgatgggaaa attcgcgctg cgcgggctcg cccagagcat ggcgcgcgaa ttgtcgccgc
     1861 agggaattca cgtcgcgcat ttcgtcatcg acggcggcat tcgcagcgcc gcgcgcaccg
     1921 aacctgagga ccggccggat tcgatgctcg accccgacgc cattgccttc agttactgga
     1981 acgtgttgca acaaccgcgc agcgcctgga cctgggagct ggaattgcgg ccgtgggtgg
     2041 agaagtttta gctttcgagt cagtgacgca gtcccacata ctgacgtcat catccgcgaa
     2101 agcggatgat ccagtattcc gcgacggctg ataggctgct gtgacggcgg cgattactgg
     2161 atacccgctg gagcctgtca tcgggctcgc caagggcgag acccggtggc gggtatgacg
     2221 gcgggggttt tagttgaacg cggggcaaac cgcgcaatgg gaggaattat atgacgacca
     2281 ccgaaacaac catcgacacc ggcacctccg aattaaagtg cgtgatccgc gaccgcgtcg
     2341 ccatcgtcac gctcaaccgg ccggaagtcc gcaatgcgtt gtcggacaat ctgacgccgg
     2401 cgctgcgcac catcatcaag gcctgcggcg agagccccga cgtcggcgcg ctgttgatca
     2461 ccggcgccgg cactgcgttc tgcgccggcg gcaacgtcaa gggcatgggc gctcaccgcg
     2521 acaagaagaa actcgccatg tcctatgacg agaaggtcgc ggacttgcag gaacggcaac
     2581 gcttgctgac cggcgcgctg gtctcggtac gcaagccgac catcgcggca ttgcccggcc
     2641 ccgcggtcgg cgcaggactg gccatcgcca tggcctgcga catccgcatc gcggcggaat
     2701 cggccttcat ttccaccggc tatctgcgga tgggtttaag cggcgactac ggcatcgctt
     2761 ggcttttgac gcgcctcgtc ggcacgtcgc gcgcgcgaga actgatgttt accgccgaca
     2821 aggtcgatgc cgcgagatgc gaggcgatcg gcctcgtcaa ccgcgtggtg ccggatgcga
     2881 agctccagga tgcggctttt gcgctggcca aatcgatggc ggaagggccg acgcaggcgt
     2941 tgcgctacat gaaggacaat ctcgacgaag ccctgtcgtt cgatttcgcc accgcgcgcg
     3001 accaagaggc cgaacgcctg atccgtttga ccacgacagc ggatcaccgc gaagcggtgc
     3061 aggcttttat tgagaaacgc aaagcggtgt tcagggggag ctaagccttc acaccgccgc
     3121 ctgaagcgcg atctgttggg ccgccagccg ccagctggcg caggaaattt cctgtggcgc
     3181 cgcgtcgata tgtttttcga agcgcaccag cttccagtcg ccggggctgt tggtgaaggc
     3241 aaaaccggat ttacgggcga gggagatcat gacgtcattg gagcgcaagg tatcgccgaa
     3301 caggcgttcg gcaccaaagg tggcggcgcg gcattgcaga ttcttcagca gtgcggaacc
     3361 gatgccgtcg ccttgccagc ggtcgtcgat cgacaggcca aactcgaaac ttaaagtctc
     3421 ggcgtcgaag gcgtagcggg cttcgccgac gatggtctcg atgccatcga tcgtcatgag
     3481 cgcgatcacg ctgaagcggc cggcctcgcc gacgtgggtg aaatgatcaa gttcggagtg
     3541 cggcagctcg ctcatggcgc cgaggaagcg attgtagcgc gagccgaccg agagcgaccg
     3601 gaagtaaccc tgcaatgcgt ccgcatcgcg cggctcgaca aagcgcacgg tcaacagttt
     3661 tccactgcgc gaccgcaaga cgtcggaatg ctgtcggagg tcgttcagtc gaagcgcgat
     3721 cactactggg ctccatcaat caagaaaagt cgtcggttgg cgtccccctt aagaggcgac
     3781 gccaacctgg cggcgttcac gcccgccaaa acggcttttc ggcttcgaaa aagatgtcag
     3841 cccgggaaag gccgacatcg tgaaggtcgc ggtcggtcag ttcggccaac cggcgacgct
     3901 catgcgcacg ctcccgccaa aggcgcaggg tttcgccgat ttgagcgaaa acgccggaac
     3961 catgacgatt tatcatcgat tcatgtgtgc aagtggacat tttcatctcc taaagctaat
     4021 ctgttgcctg caatatctgc attatcgagg ctttccgcaa acgacattgt gcgccacctc
     4081 acatgacata aactcatgta tagattgccc ggaaaatgac cgcccgattg ccatcgctca
     4141 acggactgcg cgccttcgag gccgcggctc ggcatctcag cttcacccat gcggcttccg
     4201 aactgaacgt cacccaaacc gcgatcagcc atcagatccg ccggctggag caggaactgg
     4261 gaatccgcct gttcgtccgg cagaaccggg cgctggcgct gacgccggaa gcgcgggatt
     4321 atctgcccgg ggtccgggct gcatttaacg atttgcggct cgccaccgac cggctgttgc
     4381 gcagggacaa cgaccacgtg ctgaccgtca gtacgctggc gtcg
//
LOCUS       DASWGM010000217         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1683400,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000217 DASWGM010000000
VERSION     DASWGM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683400"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VGA15_06615"
     CDS             complement(<1..630)
                     /locus_tag="VGA15_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9587404.1"
                     /translation="MQFRVSPKTLGDTSAEEWQARVDLAAAHRLAYMHGFSEGIFNHL
                     TLVVPGSSDRYYQIPFGMHWSEVTASSFMEVGIDDGKVKRGAGDVERSCYCIHAPIHK
                     ALPQAKAVFHTHMPYASALTRLEDPRIKEIGQTEVGMSGEIAYDDEYTGPALDPAEGA
                     RLARVIGDKTVLFMANHGISTVGETVADAYDRLYYIERAAQVQVYAMWTG"
     gene            821..1306
                     /locus_tag="VGA15_06620"
     CDS             821..1306
                     /locus_tag="VGA15_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9587405.1"
                     /translation="MTEESKLKLPAFDPGVVAESNFTSYPELHRTANQMRYNRRLGDH
                     AGLRNFGVNLTRIVPGGQSSYRHAHSRQDEFVLVLEGEVVLQTNDGEQTLKPGMCAGF
                     PAGSGDAHRFVNRSATDAKLLVIGDRSSGDEITYPDVDMHAVLSPEGIYRYRTKSGEP
                     L"
     gene            complement(1452..3089)
                     /locus_tag="VGA15_06625"
     CDS             complement(1452..3089)
                     /locus_tag="VGA15_06625"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX9587406.1"
                     /translation="MSTIDLAVSPSDLRTLAEQSNAWPFEQAKQIVARLKRKPKDEVL
                     FETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTEDRIRTRLLAFSDDMDGFRKVPDNL
                     PNKEMLAEHLGKPLTRVPDPFGTHSSLGAHNNARLRAFLDTFGFDYEFASSTDYYTSG
                     KFDATLLRVLERIDQVMAIMLPSLREERAASYSPFLPICPRTGVVLYVPIMAHDVKAG
                     TISYDDPQTKERVTLPVTSGHCKLQWKPDFAMRWAALGVDYEMSGKDLIDSVKLAGKI
                     CSTLGGTPPEAFIYELFLDDKGQKISKSKGNGLTIDEWLRYASPESLSLFMYREPKSA
                     KRLYFDVIPRNVDDYQQFLEGFPRQDAKQQLANPVWHIHAGHPPAADMPVTFQLLLTL
                     VSSSNAENAATLWGFIGRYRPGVTPQTHPKLDAMVGYAINYYRDFVAPTKKFREPTDT
                     ERVALQDLRDALSQLPPESTAEEIQNVVYEIGRREPFLDTKKAKDGKPGVSLDWFNML
                     YQVLLGQEKGPRFGSFVAVYGLPNAVTMIDGALARSG"
     gene            complement(3290..>3434)
                     /locus_tag="VGA15_06630"
     CDS             complement(3290..>3434)
                     /locus_tag="VGA15_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9587407.1"
                     /translation="YFGEGIGIAVRKGNDLLRQSLNWALFRLWEKGRYTDLWLRYFSV
                     SPF"
BASE COUNT          646 a         1050 c         1062 g          676 t
ORIGIN      
        1 cccggtccac atcgcgtaga cctgaacctg tgccgcgcgc tcgatgtagt aaagccgatc
       61 ataggcatcg gcgacggtct cgccgaccgt gctgatgccg tgattggcca tgaacaacac
      121 cgtcttgtcg ccgatcaccc tggcgaggcg agcgccctca gccgggtcga gcgccggccc
      181 ggtatactcg tcgtcatacg cgatctcgcc tgacatgccg acctcggtct ggccgatctc
      241 cttgatgcgc gggtcttcga gacgcgtcag cgcgctcgca tagggcatgt gggtgtgaaa
      301 cacggctttg gcctgcggca gcgccttgtg gatcggcgcg tggatgcagt agcaggaacg
      361 ttccacgtcg ccggcgccac gcttgacctt gccgtcgtcg atgccgacct ccatgaagga
      421 agaggccgtc acttcggacc aatgcatccc gaacgggatc tggtagtagc ggtcgctgct
      481 gccgggaacg acgagggtca ggtggttgaa gattccctcg ctgaaaccgt gcatataggc
      541 cagccggtgc gcagccgcga gatcgacccg cgcctgccat tcctcggcgc tggtgtcccc
      601 gagcgttttc ggcgaaaccc tgaactgcat ggacatcctc cctattggta tggcgatggc
      661 atccgcgtca ccgcttcaac gggatcccgg acggttcttg ttatgcccaa tattgtaacc
      721 tattttggag cggaattggt tgaaattggc gcgggtctct catctccatt tgcggcggct
      781 tcaccatcca gttgcagaca ggtcatgaaa aggagccgga atgaccgagg aatccaaact
      841 gaagcttccg gcttttgatc cgggcgttgt agctgagagc aatttcacca gttatccgga
      901 gcttcaccgg acagccaatc agatgcgata caatcggcgg ctcggtgatc acgccggcct
      961 gcggaatttc ggcgtcaact tgacccggat tgttccggga gggcagtcct cttaccggca
     1021 tgcgcactcg cgccaggacg agttcgtcct tgtactggag ggcgaggtgg tattgcagac
     1081 gaatgacggc gagcaaacat tgaagccggg gatgtgcgcc gggtttcctg ccggttcagg
     1141 cgacgcccac cgatttgtca atcgctccgc aaccgacgca aagctgctcg tcattggtga
     1201 ccggagttca ggtgacgaga tcacctatcc cgacgtggac atgcatgcag ttctcagccc
     1261 tgaaggcatc tatcgatacc gtaccaaatc cggagagccc ctttgagcgg ggtactccac
     1321 tcgacgtcgt cccggccgtg agccgggacc cataccgaga ggtctttcgt cttcgcgata
     1381 tggtcgacgc tgttcgcagc aaccgaggcc gatggtgggt ccctgctttc acagggacga
     1441 cgcgacagta cctaccccga ccgcgccagc gcgccgtcga tcatggtgac cgcattcggc
     1501 aagccataga ccgcgacgaa cgaaccgaag cgcgggcctt tttcctggcc gagcagcacc
     1561 tggtagagca tgttgaacca gtcgagcgac acgcccggct tgccgtcctt ggctttcttg
     1621 gtatccaaga acggctcgcg ccgaccgatc tcgtagacca cgttctggat ctcctcggcc
     1681 gttgattcgg gcggcaattg cgacagcgca tcgcgcagat cctgcagcgc cacccgttcg
     1741 gtatcggtcg gctcgcggaa tttcttggtc ggggccacga agtcgcggta gtagttgatg
     1801 gcgtagccga ccatcgcgtc gagtttcggg tgggtctgcg gcgtgacgcc gggacgatag
     1861 cgcccgatga aaccccacag ggtcgcggca ttctccgcgt tcgacgatga caccagcgtc
     1921 agcagcaact ggaacgtgac cggcatgtcg gccgcgggcg gatggccggc gtggatgtgc
     1981 cagaccggat tggccagctg ctgttttgca tcctggcgcg gaaagccttc gaggaattgc
     2041 tgatagtcgt cgacgttgcg cgggatcacg tcgaaataca gccgcttggc cgacttcggc
     2101 tcgcgataca tgaacagcga cagcgattcc ggcgacgcat agcgcagcca ttcgtcgatg
     2161 gtgagcccgt tgcccttcga cttggaaatc ttctggccct tgtcgtcgag gaacagctcg
     2221 taaatgaacg cttcgggcgg cgtgccgccg agtgtcgagc agatcttgcc cgcaagcttg
     2281 accgaatcga tcaggtcctt gccggacatc tcatagtcga cgccgagcgc cgcccagcgc
     2341 atcgcaaagt ccggcttcca ttgcagcttg caatgcccgc tggtgaccgg cagcgtgacg
     2401 cgctctttgg tctgcggatc gtcataggag atcgtgccgg ccttcacgtc gtgcgccatg
     2461 atcggcacat acagcaccac cccggtgcgc gggcagatcg gcaggaacgg cgagtagctc
     2521 gccgcgcgtt cctcgcgcaa ggacggcagc atgattgcca tcacctgatc gatgcgctcc
     2581 agcacgcgca gcaaggtggc gtcgaatttt cccgacgtgt aataatcggt tgaactggcg
     2641 aactcgtagt cgaatccgaa ggtgtcgaga aacgcgcgca gccgtgcatt gttgtgcgcg
     2701 cctaagctcg aatgggtgcc gaacggatcg ggcaccctgg tcagcggctt gccgagatgt
     2761 tctgccagca tctccttgtt cggtaggtta tccggcacct tgcggaaacc gtccatgtcg
     2821 tcggagaacg caagcagccg cgtcctgatc ctgtcctcgg tgagcacgcg aaacgcatgc
     2881 cgcaccatgg tggtgcgggc gacctcgccg aaggtgccga tatgcggcag gccggacggc
     2941 ccatagccgg tctcgaacag cacctcgtcc ttcggtttcc tcttcagccg cgcgacaatc
     3001 tgcttcgcct gctcgaacgg ccaggcgttg gattgttcgg cgagcgtacg caggtcgctc
     3061 ggactgacgg caagatcgat cgtggacatc aaagggggcc tccgggccgc ggaaactagc
     3121 gtgttagcgg acaaatgcaa aatggcgatc gaaccaccgt cattccggga tggtgcgtta
     3181 gcaccagacc cggaatctcg agattccggg ttcgatgcgg agcctgtcat cgggccgcgc
     3241 tttgcgcgga cccgttggca tcgccccgga atgacagagc ctggaattcc taaaacgggc
     3301 tcaccgaaaa atagcgcagc cacagatcgg tgtagcggcc tttctcccag agccggaaca
     3361 gcgcccagtt cagcgattgc cgcaacaggt cgttgccctt gcgcacggcg atgccgatgc
     3421 cctcgccgaa atag
//
LOCUS       DASWGM010000218         2332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1683479,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000218 DASWGM010000000
VERSION     DASWGM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683479"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VGA15_06635"
     CDS             complement(<1..235)
                     /locus_tag="VGA15_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587408.1"
                     /translation="MAIFVDPSLVMRTLSSFNHSGPDEVPIAILLRQTALKASVGIDP
                     IERRPNSGRHPGWAIPHGTRPPYAIALIQGWAKA"
     gene            228..1196
                     /locus_tag="VGA15_06640"
     CDS             228..1196
                     /locus_tag="VGA15_06640"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX9587409.1"
                     /translation="MAKHTTICAGIDTGKRKLDVAIEGRRELLQVDNTAEGHKVLLAW
                     LRQRRVKRVGIEASGGYEQPVVAELRRKRFVVVVFQPVQVRAYAKFHLQRAKNDKIDA
                     ALIAACTAAVRKIHAAPDPRLLPFAEQLTMIDQINEDIARLKNRIESCRNARIKKLWQ
                     EDIARLEMREKAELKALVASIRKRPDLAKRLELINSVDGIGLPTAVAILVRLPEIGRI
                     TREQAVALAGLAPYDDDSGEQVGVRHIEGGRKRLRRALYTASLAASFRWNRQLIQLYQ
                     RLTAAGKEHKRALVACARKMLIFANTVVARGTPWCDEPPVGSLSVS"
     gene            1605..>2332
                     /locus_tag="VGA15_06645"
     CDS             1605..>2332
                     /locus_tag="VGA15_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9587410.1"
                     /translation="MNISDLQKLTDWLIDGARSATNPPRMMEETCERMVQAGLPLWRV
                     GVFIRTLHPDIFGRNFIWRLGEEVEMGTVDFDVLDSTGFTSSPLAIVFIEGKEVRGRL
                     DELQSGRFPIFADLHAEGVTEYIALPLLFIDGSIHASSWTTKQPGGFSDQQLSALRSL
                     IPPLARVIEIVSLRRTAALLLDTYVGNRAGERILGGQIRRGHTDTMDAAIWLSDLRGF
                     TALSDRLPAETVVEILNCYFDCQVS"
BASE COUNT          449 a          700 c          725 g          458 t
ORIGIN      
        1 gcgcctttgc ccacccttgt attagcgcga tcgcgtatgg tggcctggtt ccgtgaggaa
       61 tggcccagcc cgggtgacgg cccgagttgg gtcgccgttc gatcggatcg atgcccaccg
      121 aagccttgag ggctgtctga cgtagcaaga tcgcgatcgg tacttcatcg ggacccgaat
      181 ggttgaacga actcaaggtt cgcatcacaa gggagggatc gacgaagatg gccaagcata
      241 ccacgatctg tgccgggatc gataccggca aacggaagct cgatgtggcg attgagggcc
      301 gccgcgagct gttgcaggtg gataacacgg cggaaggtca caaggttctg ctggcgtggc
      361 tgcgacagcg ccgcgtcaaa cgcgtcggca tcgaggcgag tggcggttac gaacagcccg
      421 tcgtcgctga gctgcggcgc aagcgatttg tagtcgtggt attccagccg gtgcaggtcc
      481 gggcctatgc aaaattccac ttgcaacgag caaagaacga caagatcgat gccgctctta
      541 tcgcagcctg cactgcggcg gtgcggaaga tccatgccgc acccgatcct cggttgctgc
      601 cattcgccga gcaactgacc atgatcgatc agatcaacga ggatatcgcg cgcctcaaga
      661 accggatcga gagctgccgc aatgcgcgga ttaagaagct ctggcaggag gatatcgccc
      721 gcctggagat gcgcgagaag gccgaactca aggctctggt cgcctcgatc cgcaagcgtc
      781 ccgaccttgc caagaggctt gagttgatca acagcgtcga cggcatcggg ctgccgaccg
      841 ccgttgccat cctggtgcgt ctgcctgaga tcggcaggat cacgcgggag caagctgtcg
      901 ctctcgccgg ccttgcgccc tatgacgacg acagcggcga gcaggtcggt gttcgacata
      961 tcgagggcgg tcgaaagcgg ctgcgtcgcg ccctttatac tgcttcactg gctgcatcgt
     1021 ttcgctggaa ccggcagctc atccagcttt accagcggct gaccgcggcc ggaaaggaac
     1081 acaagcgcgc gctcgttgcc tgcgcaagga agatgctcat cttcgccaac actgtagttg
     1141 ctcgcggcac gccttggtgc gacgaaccgc ctgtgggatc attgtctgtt tcttagttcg
     1201 gccgggactg tttcttcgtc ccgacctgca actcctcgtg acgtcgcgcg cagcggccgt
     1261 caaggatggc cgtcgctcgc tcgagcctca catgcatctg ccgccaggcc acgccttgac
     1321 ggccgcgagc acggcgtcaa tcctcaaggc agtcggacca aggatcaatt ccctggccag
     1381 actcttaatg gttgctacga aactgcgttt gaaatcagag tgacgtcatt gcgaggcgtg
     1441 aagcgacgaa gcaatccaga gtccgtcatt ccggggcgat gcgaagcatc gaacccggaa
     1501 tctcgagatt ccgggtctgc gcctgacggc gcatcccgga atgacggcta tcgattgctt
     1561 tgcttcgtct tgacgtctca cgaacttgcg ccgcaaatgt tctcatgaac atttcagatc
     1621 tgcagaaact caccgactgg ctgatcgatg gcgcgaggtc tgcgaccaac ccgccccgga
     1681 tgatggagga aacctgcgag cgcatggtgc aggcgggctt gccgttgtgg cgggtcggcg
     1741 ttttcatccg gacgctgcat cccgatattt tcggccgcaa tttcatctgg cggctgggcg
     1801 aagaggtcga aatgggcacg gtcgatttcg atgtgctgga ttcgacggga ttcacaagca
     1861 gtccgctggc gatcgtcttc atcgaaggaa aagaggtcag gggccgcctc gacgagctgc
     1921 agagcggacg ctttccgatt ttcgccgatc tgcatgccga aggcgtcact gaatacatcg
     1981 cgttgccgtt gctgttcatc gacggctcga tccacgcatc gagctggacc accaaacagc
     2041 cgggcggctt cagcgaccaa caactgagcg cgctgcggtc gctgatcccg ccgctggcgc
     2101 gggtgatcga aatcgtcagc ctgcgccgca ccgccgcgct cctgctcgac acctatgtcg
     2161 gcaaccgcgc cggtgagcgg attctcggcg gacaaattcg ccgcggccac accgatacca
     2221 tggatgccgc gatctggctg tcggacctgc gcggctttac cgcgctgtcg gaccggctgc
     2281 cggccgagac cgtggtcgag attctcaatt gctattttga ctgccaggtg tc
//
LOCUS       DASWGM010000219         9618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_849739,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000219 DASWGM010000000
VERSION     DASWGM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849739"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VGA15_06650"
     CDS             complement(<1..1103)
                     /locus_tag="VGA15_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX9587411.1"
                     /translation="MAKNPDHPKKPVKTPKSKAHRPDVQPIGPALAELLNPAINRGDA
                     GLGSGTGLQPPPDNSWDRRSGGEAAAHRARASTRGTSEDVAKRDASLTGTTSRGSPPP
                     NPPPQAGEGSKRLAPSADERDFSTESQPASPLPLAGEGQGRGASAKQGFDEAPQANYG
                     TAATIPVLDPELARHLGFTTEEEDAAALARPPRNKMEALGVAATADALENLIREGRPE
                     FKGEDGQVKIWTPHRPPRPEKSEGGVRFVIKSEYEPKGDQPTAIAELVEGIKRNDRMQ
                     VLLGVTGSGKTYTMAKVIEATQRPAIILAPNKTLAAQLYGEFKSFFPDNAVEYFVSYY
                     DYYQPEAYVPRTDTYIEKDSSINEQIDRMRHSAT"
     gene            complement(1415..1663)
                     /locus_tag="VGA15_06655"
     CDS             complement(1415..1663)
                     /locus_tag="VGA15_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587412.1"
                     /translation="MNYDGFQIESFEAGKGLWHARIRRADQEPVVIDGVLFSTLEVGF
                     AWSSPDAAVADAKTQIDHLNQRYAAVGRTTQAECPVAS"
     gene            2013..5168
                     /locus_tag="VGA15_06660"
     CDS             2013..5168
                     /locus_tag="VGA15_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9587413.1"
                     /translation="MRLSTRLTVAMVALVLLATTAVGVLTYRNIAAFALPRALDRIDT
                     HAQLLASELAASVRGARADVIGFRSSNAVIDIMTAHLNRGIDPAAAATEAEWRRRLGL
                     RFAAELISKPNYHVFRYIGADDGGRELVRVERSDPGGPPRIVPDSELQPKGHRDVFKQ
                     TIQLPPGGVYVSPVDFNIETPRIPVLRVATPVHAPDGRPFGIVVINVDMRPALARIRS
                     AATKGGRSYVVNDQGDYLVHPDPNREFGFEFAKPLHIQDDFPEFSQILATGDTAPRVI
                     QDRAGTRFGIGVVTERLADGPRVFVIQAVPYSTLMAATTAVRDSSLMGGFVAAFCAFL
                     LAIIVARSLTRPLVQMTKVVQGFSRDETVALPTGGGHEISVLADAFAHMAAESRAKTA
                     ALNREIEERRRTFEREQLFIAAVESSNDAIVTETLDGVITGWNPAAERMFGFTAREAI
                     GNSIDIIVPCDLQREAHATLDRIRGGEKIDYHETVRTSKDGRQIDVSLGISPIRSQSG
                     TIIGAAQVARDITARKKAQEALLESEQMARDVIANAFEAFIQTDEAGHILEWNPQAEA
                     IFGWSRQEALGKHPTSLLLSEALKPQFQTMSDRLVRNEQNAAAGARFEIEAMRKDGRT
                     VKIEVSLKALRRRSGYVFNAFVRDLTQKIAAEEQLRQAQKMEAVGQLTGGIAHDFNNM
                     LTVITGTIEILAEGVADKPHLVTIANLISDAADRGAELTSHLLAFARKQPLQPRETDI
                     NRLIVDSAKLMRPTLGDHIEIVSMLADSAGAALVDPGQLSTVLLNLAINARDAMPSGG
                     KLTLETRNVTLDSIYAAANGDIQAGNYVMIAVSDTGTGIPEAIRDRVFDPFFSTKEVG
                     KGTGLGLSMVYGFVKQSGGHIKVYSEVGFGTTFKLYLPQADAPPEQRAVEPLIPEMEG
                     GDETILVVEDDPLVRAYVNTQLQSLGYKTLSAANGVEALAIVDSGAAFDLLFTDVIMP
                     GRLNGRQLAAEMEQRHSSLKVLFTSGYTENAIIHHGRLDSGVLLLAKPYRKLDLARLL
                     RVALTSAGNVPDGSPPPEPRG"
     gene            complement(5266..6492)
                     /locus_tag="VGA15_06665"
     CDS             complement(5266..6492)
                     /locus_tag="VGA15_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9587414.1"
                     /translation="MPLEQVQTLIIGGGQAGLTMSHRLKQRGLKHLVIERHRIAERWR
                     SERWDGLRFQFPNWSVRLPDFPFPHSDPDGFATSREIVDFIGAYADFIAAPIRCGVAV
                     TALRRRDGGSGLIAETSDGSIEADNVVVATGPYQSPVMPALLRDDINLFQVHASRYLN
                     PDQLPPGAVLVVGAGASGSQIAEELSRAGRRVYLSVGRHTRLPRRYRGRDLIWWLSAM
                     GIDQTAVEQRGPSRLLPLITGAYGGHTIDFRRFAADGVTLLGRVKAAHDGIIDLAPDL
                     AESLANGDAMYASFLDRVDAHIEHHALAIPQDPDARAVLSDPPCIADPLRRLDISADG
                     IGAVIWATGYGVDFGWIDVPVLDARGEPVHRGGITGVPGLYFLGLQWLSKMRSSFLSG
                     VGDDAADLADHIAARS"
     gene            complement(6845..7762)
                     /locus_tag="VGA15_06670"
     CDS             complement(6845..7762)
                     /locus_tag="VGA15_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9587415.1"
                     /translation="MRRLLFLNGIKAFEAAARSGSFAAAGSELNVSAAAISRMVHLLE
                     QRLGVALFERKANRLAMTSAGRAYQNGLTPIFDALASLTAQVTAPSSVRVLTIGVGPT
                     FAMKWLIPRLADFRKHQPDIDVWITTGGAAAPFGDDWSCGIKLGDGQWPGLVSEPLFA
                     ADLLPVCAPRLALQLKRPGDLKGPSLLRVAHSPDDWPLWLKTSGVPRVTARGPEFQFY
                     GQALQAAVDGLGIAMGIRPYIDDDLAAGRLVAPFALSVPKGMRWYLVYRSFHTEQRDF
                     AAFRRWIVRAVAGPARGADNAKAIKASRR"
     gene            7851..8612
                     /locus_tag="VGA15_06675"
     CDS             7851..8612
                     /locus_tag="VGA15_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9587416.1"
                     /translation="MTPLLIGALGLLMVATAFLSGLFGMAGGLILIGALLTLMPLPSA
                     MVLHAITQMASNGWRAFLWRSHIRWRPVAIYLIGCAVALAAWSITRYVPDKPVALLLL
                     GVTPFMARLTPPGLKPNPESLWQGTFYGSICMGLMLMTGVSGPLMDTFFLGGNFDRRG
                     IVATKATCQVASHFVKLIYFGGIIDQAATLDPVLAGVAVAASMLGTTLARRILEAMTE
                     LQFRTWARRLITTVAGYYILYGSWLLLVRSNAFAF"
     gene            8630..8866
                     /locus_tag="VGA15_06680"
     CDS             8630..8866
                     /locus_tag="VGA15_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587417.1"
                     /translation="MADTADPLVLDFVEWVAREPRLYSEVIATWRTSCPRLTIWEDAT
                     DGGYVARETIAGFGIIVTVTEDGEKFLREHGRIG"
     gene            9053..>9618
                     /locus_tag="VGA15_06685"
     CDS             9053..>9618
                     /locus_tag="VGA15_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9587418.1"
                     /translation="MIDLHYWTTPNGHKITMFLEETELKYKIFPVSIGKGDQFKPEFL
                     AIAPNNRIPAMVDHEPKGGGKPISIFESGAMLLYLAEKTGKFLPADIYGRYDAIQWTF
                     WQMGGLGPMAGQNHHFSNYAQEKIKYAIDRYVNETNRLYGVLNKRLSNREFIAGDYSI
                     ADMASYPWVVPYKNQSQNIDDFPHLKRWL"
BASE COUNT         1843 a         2969 c         3023 g         1783 t
ORIGIN      
        1 gtcgccgagt ggcgcatgcg gtcgatctgc tcgttgatcg aagagtcctt ctcgatatag
       61 gtgtcggtcc gcggcacata ggcctcgggc tggtagtaat cgtaatacga gacaaaatac
      121 tcaacggcgt tgtcggggaa gaagctcttg aactcgccat agagctgcgc cgccagcgtc
      181 ttgttcgggg cgagaatgat cgccgggcgc tgcgtggcct cgatcacctt ggccatggtg
      241 taggtcttgc cggagccggt gacgccgagc agcacttgca tgcggtcgtt gcgcttgatg
      301 ccctcgacta gctccgcgat cgcggtcggc tggtcgccct tcggctcgta ttcggatttg
      361 atcacgaagc ggacgccgcc ttcggatttc tccgggcgtg gcggacgatg cggcgtccag
      421 attttcacct ggccgtcttc gcccttgaat tcgggccgtc cctcgcggat cagattttcc
      481 agcgcatccg cggtagcggc aacgccgagc gcctccatct tgttgcgcgg cggccgcgcc
      541 agcgccgcgg cgtcctcctc ctcggtggtg aagccgagat gccttgccag ttccggatcg
      601 agcacaggaa tggtggcggc cgtgccgtaa ttggcctgcg gggcttcatc gaagccttgc
      661 tttgcggaag cacccctacc ctgaccctct cccgcaagcg ggagagggga cgcaggttgc
      721 gactccgtgg aaaaatctct ttcgtcagca ctcggtgcca gccgcttgga gccctctccc
      781 gcttgcgggg gagggttggg tgggggtgag ccacgggagg ttgtgcccgt gagcgaagcg
      841 tcccgcttcg ccacatcctc gctggttcca cgggtcgacg cccgcgcgcg atgcgctgcg
      901 gcctcgccgc cggagcggcg atcccaggaa ttatccggcg gcggctgcaa tccggttcca
      961 gagccaagcc cggcatcgcc gcgattgatc gcgggattga gcagttccgc cagcgccggc
     1021 ccgatcggct gcacgtcggg ccggtgcgcc ttcgatttcg gggttttgac gggctttttc
     1081 ggatggtccg gattcttcgc cataagccga atatgggatg agtccggcgg cgagaaaagg
     1141 gcaaaagccg acagcgaatt agcgcctgcc cggcgatatc aaagaatggc tggtgttcat
     1201 caacttgagt ctctgaggcc cgaacggacc gcatcctggt ggatatggcc gtatttcggg
     1261 catcgcaagg tccaatattc aagctctgat ggtccgggga taatgcgctg caccaccaga
     1321 cgggtgtcgc agaggggaca attggagcgc accatcgctg acggtaacgg agtcggctcg
     1381 gcttcagatt tgcacatagg aatgcgggct actgttagga agcaacagga cattccgcct
     1441 gcgtcgttcg cccgacagcc gcgtatcgct ggttgaggtg gtcgatttga gtcttcgcat
     1501 cggctaccgc cgcatcggga ctggaccacg cgaagccgac ttcgagcgtg gaaaacaaaa
     1561 cgccatcgat gacgacaggt tcctgatccg cccgcctgat cctggcatgc cacaaaccct
     1621 ttccggcctc gaaggactca atttgaaagc cgtcatagtt cattgtcgtg actccaggtt
     1681 cttttggcag agagtatttt ccggagccga aacaaccatg agcccaccaa aaccaatgtg
     1741 aactcattca cacgccgggc ttatttttca ggcctgcaaa acaggacagt tgcgccagcc
     1801 atgacgcatt agtccgaatc cgaaccaagc gtacggcgat gtcgtcggcg agatcagcca
     1861 acgcccatca ggttgcgcaa ctcgttaaac gagtcataga tcgattcgca tgcgccgccg
     1921 tgccagaaag attaccgcgg tcggccgatt cgagatagac cacggcccaa cttggcgctg
     1981 acgccgatga aggtgccgcc cagagggcaa tcatgaggtt gtcgactcgg ttaaccgttg
     2041 cgatggtcgc gttggtgctg ctggcgacga cggcggtcgg ggtgctgact tatcgcaata
     2101 tcgcagcgtt tgccctgcct cgggcattgg accggatcga tacgcatgct cagctgctcg
     2161 ccagcgagct tgcagcctca gtccgcggcg cgcgcgccga tgtgattggc tttcgctctt
     2221 ccaacgccgt catcgacatc atgacggcgc acctcaatcg cgggatcgat ccagcggccg
     2281 ccgccaccga ggcggagtgg cgaagacgcc tgggattgcg cttcgctgcg gaactcattt
     2341 ccaagccaaa ttatcacgtg ttccgctaca tcggggccga cgacggcggg cgcgaactcg
     2401 tgcgggtcga gcgttcggat ccaggcggcc cgccgcgcat cgttccggac agcgagttgc
     2461 agcccaaggg ccatcgtgat gtctttaagc aaaccatcca gttaccgccc ggcggggtct
     2521 atgtctcgcc ggtcgatttc aacatcgaaa cgccgcgtat cccggtgctg cgcgtcgcga
     2581 cgccggtgca cgcgccggac gggcggccgt ttggcatcgt ggtcatcaac gtcgatatgc
     2641 ggccggcact tgcccggatc cgctcggccg ccaccaaagg cggtcgaagc tatgtcgtca
     2701 atgaccaggg tgattatctc gtccatcctg accccaatcg cgagttcggg ttcgaatttg
     2761 ccaagcccct tcatatccag gacgactttc cggaattctc ccaaatactc gcgaccggcg
     2821 atacggcgcc gcgcgtgatt caggatcgag ccggcacgcg cttcgggatc ggtgtggtga
     2881 ccgagcgtct ggcggatggt ccccgggtct tcgtgatcca ggcggtgccg tattcgacac
     2941 tcatggcggc aaccaccgcg gttcgcgact ccagcttgat gggtggattc gttgcggcgt
     3001 tttgtgcatt cctgctggcc atcatcgtcg cccgctcgct gacgcggccg ttggtgcaga
     3061 tgaccaaggt cgtccagggg ttttcccgcg atgaaacggt cgcattgcca accgggggag
     3121 gccacgagat cagcgtgctg gccgatgcgt ttgcacacat ggcggccgag tcgcgcgcca
     3181 agacggcagc actcaatcgg gagattgaag agcgccgtcg caccttcgaa cgcgagcagc
     3241 tcttcatcgc agccgtggaa tcgtccaacg atgcaatcgt gaccgaaacc ctcgatggcg
     3301 tcatcaccgg gtggaatccg gccgcggaac gcatgttcgg atttaccgcc cgggaagcga
     3361 tcggcaatag catcgatatc atcgttccct gcgatctgca gcgcgaggct cacgccaccc
     3421 tggacagaat acgaggtggc gaaaaaatcg attaccacga aaccgttcgc accagcaaag
     3481 atggacggca gatcgacgtg tccctcggca tctcgccgat ccggtcgcaa tcgggaacga
     3541 tcatcggcgc tgcccaggtt gcgcgcgata ttaccgcgcg aaagaaagcg caggaggcgc
     3601 tgcttgaaag cgagcagatg gcacgcgacg tcatcgccaa tgctttcgaa gcgttcattc
     3661 agaccgacga agccggccac attctcgaat ggaatcccca ggccgaggcg atcttcggat
     3721 ggtcgcggca ggaggcgttg gggaagcacc cgaccagtct gctgctgtcg gaagcgctta
     3781 agccgcaatt tcagaccatg agtgatcggc tggtgcggaa cgagcagaac gcggccgccg
     3841 gcgcgcgctt tgaaatcgag gcgatgcgga aggatggacg taccgtcaag atcgaagtgt
     3901 ccttgaaggc gcttcgccgc cgcagtggct acgtattcaa cgcattcgta agggatctga
     3961 cccaaaagat cgcggcggag gagcaactga ggcaagccca gaagatggaa gccgttggcc
     4021 agcttaccgg cggcatcgcc catgacttca acaatatgct caccgttatc accggcacca
     4081 tcgaaattct ggccgaaggg gtcgccgaca agccgcacct tgtgacgatc gctaacctga
     4141 ttagcgacgc ggccgatcgc ggcgcggaac tcacctcaca tctcctcgcc tttgcccgca
     4201 agcaacctct gcagccgcgc gaaaccgaca tcaaccgctt gatcgtcgac tcggcgaagc
     4261 tgatgcggcc aacattgggc gaccacatcg aaatcgtatc gatgctggcg gattctgccg
     4321 gcgcggcttt ggtcgatccg gggcagctca gtacggtgct cctcaacctt gcgatcaacg
     4381 cccgcgatgc catgccgagc ggcggcaagc tcactctgga aacaaggaac gtgacgctcg
     4441 acagcattta tgccgcagcc aacggcgaca tccaggcggg gaattatgtg atgatcgcgg
     4501 tgagcgatac cggcaccggg attccggagg cgatccgcga cagagtgttc gatccgttct
     4561 tttcgacgaa ggaggtaggc aagggaacgg ggctggggtt gagcatggtc tacggtttcg
     4621 tcaagcagtc gggcggacac atcaaggtct acagcgaagt gggctttggg acgacgttca
     4681 agctctatct gccgcaggcg gatgcgccgc cggagcagcg cgcggtggaa ccgttgatcc
     4741 ccgagatgga aggcggcgac gagaccatcc tggtcgtcga ggatgatccg ctggtgcgtg
     4801 cttacgtcaa tacgcagctc caaagcctcg gctacaagac tctgtcggcc gcaaacggcg
     4861 tcgaagccct tgcgatcgtc gatagcggcg cagcttttga ccttctgttt accgatgtca
     4921 tcatgcccgg gcgcttgaac ggccggcagc tcgccgcaga gatggagcag cgacactcgt
     4981 cgttgaaagt gttgtttacg tcaggctata ccgaaaatgc catcatccat catggcagac
     5041 tggattccgg ggtccttctg ctcgccaagc catatcgcaa gctggatctg gcgcgcttgc
     5101 tccgcgtcgc attgacttct gccggaaatg ttccggacgg aagcccgcca cccgaaccgc
     5161 gcggctgagc cttgagatcg ccgtttgcga aggaagtagg gcgcggactt ctgtctttca
     5221 accgcatcgg catttccacg cagtcggcgc caagcccaac cgcaatcaac tacgcgcggc
     5281 gatgtgatcg gcgagatcag ccgcatcgtc gccgacaccg gacaggaacg aagatctcat
     5341 cttggatagc cattgcaggc cgaggaaata caggccgggc acgccggtga tgccgccgcg
     5401 gtgaaccggc tcgccgcgcg catccagtac cggaacgtcg atccagccga agtcgacgcc
     5461 gtagccggtg gcccagatca ccgcaccgat gccatccgcg ctgatatcga gccgccgcag
     5521 cggatcggca atgcaaggcg ggtccgacag caccgcgcgg gcgtcgggat cttgcggtat
     5581 ggcaagcgcg tggtgctcga tatgcgcatc caccctgtca aggaaggatg cgtacatcgc
     5641 atcgccattg gccaggcttt cggcgagatc cggcgcaagg tcgatgatgc cgtcatgcgc
     5701 cgccttcacc cgtcccagca gcgtcacgcc gtccgcggcg aagcggcgga agtcgatggt
     5761 gtggccgcca taggcgcccg tgatcagcgg cagcagtcgc gagggcccgc gctgttccac
     5821 cgcggtctga tcgatgccca tggcgctcag ccaccagatc agatcgcggc cgcggtatcg
     5881 gcgcggcagg cgggtgtgcc ggccgaccga gagatagacg cggcgacctg cgcgcgacag
     5941 ttcctcggca atttgtgaac ctgaggcgcc cgcgccgacc accagcaccg cgcccggtgg
     6001 aagctggtcg ggatttagat agcggctggc gtgcacctga aagaggttga tgtcatcgcg
     6061 caacagggcc ggcatgacgg ggctctggta gggaccggtc gcgacgacga cattgtcagc
     6121 ctcgatcgaa ccatcagatg tttcggcaat caggccggag ccgccatcgc ggcgtcgtaa
     6181 cgcggttact gcgacaccgc agcggatcgg cgcggcgatg aaatcagcat aggcgccaat
     6241 aaaatccacg atctcgcggc tggtcgcaaa gccatcggga tcgctgtgag gaaacggaaa
     6301 atccggcagc cgcaccgacc agttgggaaa ctgaaaccgc aggccatccc agcgctcgct
     6361 ccgccagcgt tcggcgatcc ggtgccgctc gatcacgaga tgcttaaggc cgcgctgctt
     6421 gagccggtgg ctcatcgtca gcccggcctg gccgccgccg atgatcagcg tctggacttg
     6481 ctcaagcggc attgcggcgc ccccggcgac gcaacctcac cggatgatgc tgttgcggcg
     6541 cgcgcacggt gcacaaaatg gaggtcggca tgagccggag gatcggcgaa atccgtggcg
     6601 gttgctagtc gtgatccgga gaattggtat cagaatggcg cgcgcaggat aggagtggtc
     6661 cgaagcggac aagcctcagt tcgcaaccat gtggtgatcc tccagtcgga cggtgaccat
     6721 gtcgaaacgc gattgcgcca gcaagcggac gctaacccgg tgaaagccga tcttgaggta
     6781 ttttttgtca acgtgaatag gtagccgtca ttgtggctgt cgtatttcgc caggatgagg
     6841 tgtttcacct tcgggacgct tttattgcct tcgcattatc ggcgccccgg gcgggtcccg
     6901 ccacggcgcg cacgatccag cgccggaacg cggcgaaatc gcgctgctcg gtgtggaagc
     6961 tgcgatagac cagataccat cgcatgcctt tcggcacgct cagcgcaaaa ggcgcgacca
     7021 gccgtccggc ggcgagatcg tcgtcgatgt agggccggat gcccatcgcg atgccaagcc
     7081 cgtcgacggc ggcctgcagc gcctggccgt aaaattgaaa ctccggcccg cgcgccgtga
     7141 cgcgtgggac gcctgatgtc ttgagccaca acggccagtc atcgggagaa tgcgcaacgc
     7201 gcaacagact tggccctttc agatcgccgg ggcgtttcag ttgcagcgcc agccgcggcg
     7261 cgcacaccgg cagcagatcg gccgcgaaca agggttcaga gaccagaccc ggccactggc
     7321 catcgccgag tttgatgccg cagctccaat cgtcgccgaa cggcgccgcc gcgccccccg
     7381 tggtgatcca cacgtcgatg tcgggctggt gtttgcgaaa atccgccagc cgcgggatca
     7441 gccatttcat cgcaaaggtc ggaccgacgc cgatggtcag cacacggacg ctggacggcg
     7501 ccgtcacctg cgcggtcagg ctggccagcg cgtcgaagat cggcgtcagc ccgttctgat
     7561 aggcgcggcc ggccgatgtc atcgccagcc ggttggcctt gcgctcgaac agggcgacgc
     7621 cgagccgctg ctccaagaga tgcaccatcc ggctgatcgc cgccgccgac acgttcaatt
     7681 ccgagccggc ggcggcaaag ctcccgctcc gcgccgccgc ctcgaacgcc ttgatgccgt
     7741 tgagaaacag cagccgccgc attccaccct caggaaaact tgtgccaggc caagataact
     7801 cagtttgcgc gagccgcaca agcaaggcag aaaagacgag gggatttcga atgacaccgc
     7861 tgctgattgg agcacttgga ctgctgatgg tcgcgacagc gtttctgtcc ggcctgttcg
     7921 gcatggccgg cgggctgatc ctgatcggcg cattgctgac cctgatgccg cttccctccg
     7981 cgatggtgct gcacgcgatc acgcagatgg cttccaacgg ctggcgcgct ttcctgtggc
     8041 gcagccacat aaggtggcgg ccggtcgcga tctatctgat cggatgcgcg gtggcgctgg
     8101 cggcgtggtc gatcacccgc tatgtgccgg acaagccggt cgcgctgctt ctgctggggg
     8161 tgacgccgtt catggcgcgg ctgacgccgc cgggcctcaa gcccaacccg gagagtcttt
     8221 ggcagggcac gttctatggc tccatctgca tggggctgat gctgatgacc ggcgtctccg
     8281 ggccgctgat ggatactttc tttctcggcg gcaacttcga tcgccgcggc atcgtcgcga
     8341 ccaaggccac ctgtcaggtg gccagtcatt tcgtgaaact gatctatttc ggcggcatca
     8401 tcgatcaggc cgccacgctc gatccggttc ttgccggcgt cgccgtcgcg gcatcgatgc
     8461 tcggcacgac gctggcgcgg cgcatcctgg aggccatgac cgagctgcaa tttcgcacct
     8521 gggcgcggcg gctgatcacg actgtcgccg gctattacat cctttacggc agctggctgc
     8581 tgctcgtgcg cagcaacgct tttgcctttt gagaggggga gcaagctcga tggccgatac
     8641 ggcagatccg ctggtgctcg attttgtcga atgggtcgcg agggagccgc ggctttattc
     8701 cgaggtgatc gcgacctggc gcacgtcatg tccgcgtttg acgatctggg aggacgcgac
     8761 cgacggcgga tacgtcgcgc gcgaaaccat cgcaggcttc gggattatcg tgacggttac
     8821 cgaagacggc gagaaattcc tgcgcgagca cggccgcatc ggctgatctc ggccgcgtca
     8881 gccgatgggc gcagccgcgc gcgcgagatg tccgtccgtg cgattgtctt caatcacttt
     8941 cgtgccccgc cgcgccaacg ccattgactc ggcaccgcgt tctggcaaca tccatgcaat
     9001 cgcatccaat ttggatcgcc ggtataaacg cgcagcagga caggaacacc ccatgatcga
     9061 cctccattac tggaccacgc cgaacgggca taagatcacg atgtttctcg aggagaccga
     9121 gctgaaatac aagattttcc cggtcagtat cggcaagggc gatcagttca agccggagtt
     9181 tctggcgatc gcgccgaaca atcgcattcc cgcaatggtc gatcatgagc cgaagggcgg
     9241 cggcaagccg atctcgattt ttgagtccgg cgcgatgctg ctgtatctcg ccgagaagac
     9301 cggaaagttc ctgcccgccg acatctacgg ccgctacgat gctatccaat ggacgttctg
     9361 gcagatgggc gggctcgggc cgatggccgg ccagaaccat cacttctcaa actacgccca
     9421 ggaaaaaatc aaatacgcca tcgaccgcta tgtgaacgag accaaccggc tctacggcgt
     9481 actcaacaag cggctctcga accgtgagtt catcgccggc gactattcga tcgccgacat
     9541 ggcgagctat ccctgggtcg tgccgtacaa gaaccagagc cagaacatcg acgacttccc
     9601 gcatctcaag cgctggct
//
LOCUS       DASWGM010000220        12807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1400600,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000220 DASWGM010000000
VERSION     DASWGM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12807
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400600"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VGA15_06690"
     CDS             <1..437
                     /locus_tag="VGA15_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9587419.1"
                     /translation="LHFNVCLAYIREFSNTLGEVLGREGRPPPDLAWAVGEAAVILVQ
                     LVEPMMPHLAEECWTVLGQPGLISEAQWPQIERDLLVEDTVTLVVQVNGKKRGEVTVA
                     RNAQNPEIEAAVLALDAVKQALGGKAVRKVIIVPMRIVNVVG"
     gene            424..975
                     /gene="lptE"
                     /locus_tag="VGA15_06695"
     CDS             424..975
                     /gene="lptE"
                     /locus_tag="VGA15_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX9587420.1"
                     /translation="MSLASIRIAARLLVVAGLAALTAGCFQPMYAEHTDGTPGLREKL
                     MGVEVPPVDKPNASQEARIQVEIRNALAFKLYGNATGMPPTHRLVLKFTTSRSSLMID
                     PATALPTSENFGIDAQYNLVEIASNKSVMTGATFSRVSYDIPGQLQRFARSRAFRDAE
                     DRAAQEIAERIQTRLASFFVAGT"
     gene            1091..2119
                     /gene="holA"
                     /locus_tag="VGA15_06700"
     CDS             1091..2119
                     /gene="holA"
                     /locus_tag="VGA15_06700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX9587421.1"
                     /translation="MVALRGKDIDAFLARPDPGRPIILLYGPDSGLVRERAEALLASA
                     VDDPNDPFSLVRLDGDELSAEPSRLVDEAMTVPLFGGRRAIRVRAGSRSFASGVDTLA
                     DSPLKDCRIVIEAGELRPESPLRKACERAKTAVAIGCYPDGERDLAKLIDDELRVSNL
                     KIAPDARAVLMALLGGDRQASRNELRKLTLYAHGASEVMLEDVMAVVADASELKIDPI
                     VDGAFAGRPDLVETEFAKAMIAGTYPGMIISAAQRQAAWLHKSALAVAEGTPLSTLLD
                     SGFPRLHFSRKGNVETALRNFSAARLVLIIEQLATAALEVRKQAALAAVIAQRTLLSI
                     AVNARRRG"
     gene            complement(2306..3184)
                     /locus_tag="VGA15_06705"
     CDS             complement(2306..3184)
                     /locus_tag="VGA15_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX9587422.1"
                     /translation="MADDARSRLGRGLASLIGDVGGEAKHQDRPRTQRKVPIEFLKPN
                     PRNPRRDFSDAELGELADSIRQHGVIQPIVVRPVKGAQDRFEIIAGERRWRASQIVGL
                     HEVPIVPVDVSDSDALEIAIIENVQREDLNGMEEAQGYHALANEFKRSQDEIAKIVGK
                     SRSHVANMMRLTKLPAEVQAYIALGQLSAGHARALIGVPDPAAAAKRIVEEGLNVRQA
                     EALAHVEGVPERKPQKARGGKLKDPNTVALEKRVSDALGLAVNVDHRGTGGTVHVRYG
                     DLDQLDEIVRRLETGS"
     gene            complement(3353..4204)
                     /locus_tag="VGA15_06710"
     CDS             complement(3353..4204)
                     /locus_tag="VGA15_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9587423.1"
                     /translation="MTVIDEVYQEDKVPSPAHHPRILALANQKGGVGKTTTAINLGTA
                     LAAIGENVLIVDLDPQGNASTGLGIDRRNRSCSTYDVLIGEAPLRDAIVPTAVPRLHI
                     ATSTMDLSGLELELGATRDRAFRLRNAIAGLNTSVTPDSDYTYVLVDCPPSLNLLTVN
                     AMAASDSILVPLQCEFFALEGLSQLLQTVEQVRSTLNPNLTIHGIVLTMFDSRNNLSN
                     QVVADVRQFMGNKVYNTMIPRNVRISEAPSYGKPVLVYDLKCVGSEAYLKLATEVIQR
                     ERDLRPH"
     gene            complement(4201..4902)
                     /gene="rsmG"
                     /locus_tag="VGA15_06715"
     CDS             complement(4201..4902)
                     /gene="rsmG"
                     /locus_tag="VGA15_06715"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX9587424.1"
                     /translation="MAQRSHQAPASTGSEADKAAALALTPVSHETEARLDRYVDLLLE
                     WQAKTNLVAPSTLPNLWTRHIADSLQLLTLAPSAKVWVDLGSGGGFPGVVLACALAET
                     SGAMVHLVERNAKKAAFLREALRISTSPGIVHLAEIGDSVDRIAGQIDCVTARAVAPL
                     HQLIRFAEPLVKRGAKALFLKGQDVEAELTEATKYWNIKPQLHSSRTGGQGWIVELDH
                     IERRNPSATAHGTRA"
     gene            complement(5325..7208)
                     /gene="mnmG"
                     /locus_tag="VGA15_06720"
     CDS             complement(5325..7208)
                     /gene="mnmG"
                     /locus_tag="VGA15_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX9587425.1"
                     /translation="MIARPESFDVIVIGGGHAGCEAASAAARMGAKTALVTHRFATIG
                     AMSCNPAIGGLGKGHLVREVDALDGLMGRVADVAGIQFRMLNRRKGPAVRGPRAQADR
                     KLYAAAMQAAILETANLRVIEGEADELLVSNGRVTGIRVADRSELSAGAVVITTGTFL
                     RGLIHLGERNWPAGRVDEAPAMGLSKSFERAGFTLGRLKTGTPPRLDGRTIDWSAVEM
                     QPGDDPPEPFSVMTDRITTPQIQCGITRTTPATHDVIRANVHRSPMYSGQITSSGPRY
                     CPSIEDKIVRFGDRDGHQIFLEPEGLDDTTVYPNGISTSLPEEVQLAILATIPGLERV
                     RMVRPGYAIEYDHVDPRELDPTLQTKRLLGLFLAGQINGTTGYEEAAAQGLVAGLNAA
                     LTAGGTDAIVFDRADGYLGVMIDDLVTRGITEPYRMFTSRAEYRLTLRADNADQRLTD
                     KGIALGCVGQARAIRHGTKMAALDAAKTLAKSLTLTPNEAARHGLVLNKDGHRRSAFE
                     LLAYPEIGWKELREIWPDLSAIGSSIAIHLEIDAKYDVYLKRQTADVDAFRRDEGLIL
                     TDVDYTDVPGLSNEARSKLEAARPRTVGQAGRLDGLTPAALGILAAYLRREARRKHSV
                     ATA"
     gene            complement(7698..9092)
                     /gene="mnmE"
                     /locus_tag="VGA15_06725"
     CDS             complement(7698..9092)
                     /gene="mnmE"
                     /locus_tag="VGA15_06725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX9587426.1"
                     /translation="MHPRDQTIFALSSGRPPSAISIARVSGPRAGAALTLLAGKIPAP
                     RMATRALLRDRNRQPIDDAVILWFPAPASATGEDVAEFHVHGGRAVLAALFAALSDFD
                     NLRAAEPGEFTRRAFENGKLDLTEAEGLDDLIHADTDRQRRQALSQLKGLLGDRARDW
                     RAQIIEASALIEAGIDFSDEADVPAELIAPALKKIKALLGEIRQVLAARGHGERLREG
                     LVVAIAGPPNVGKSTLMNQLARREVAIVSPHAGTTRDVIEVQLDLDGYPVTVIDTAGI
                     RETDDPVEQEGVRRARARAAEADLVLWLVDAHDEKNLEKSLEQGAAPAWVVRNKIDLD
                     AVGSDAAVLGIDALNPGQPAGVLGQTQRHTDFEISASGGDGIQELIAALIVFAQNYFG
                     AGEGGLISRERQRKLLQETAVSLQRSLSVIPEGEELAAEDLRMAARSLGRLLGRVDVE
                     DVLDVIFREFCIGK"
     gene            complement(9387..10652)
                     /gene="rho"
                     /locus_tag="VGA15_06730"
     CDS             complement(9387..10652)
                     /gene="rho"
                     /locus_tag="VGA15_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX9587427.1"
                     /translation="MREMKLQDLKAQTPAELVSFAEEKGVENASTMRKQELMFAILKQ
                     LAIQETDIIGEGVVEVLSDGFGFLRSPDANYLPGPDDIYVSPSQIRRFGLRTGDTIEG
                     HIRSPKEGERYFALLKVNTLNFEDPEKSKHKVNFDNLTPLFPDQRFRLELEDPTRKDL
                     SARVIDIVAPIGKGQRALIVAPPRTGKTVLMQNIAHSITANHPECYLIVLLIDERPEE
                     VTDMQRSVKGEVVSSTFDEPAVRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRA
                     YNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALVDTGSRMDEVI
                     FEEFKGTGNSELILDRKVSDKRTFPAIDISRSGTRKEELITDPQLLKKMYVLRRILNP
                     MGTMDAIDFLLDKLRNTKNNSEFFESMNT"
     gene            complement(10912..11337)
                     /gene="hemJ"
                     /locus_tag="VGA15_06735"
     CDS             complement(10912..11337)
                     /gene="hemJ"
                     /locus_tag="VGA15_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEX9587428.1"
                     /translation="MLYEWIKALHVIAVISWMAGMLYLPRLFVYHCESEAGSKQSETF
                     KVMERRLLRAIINPAMIVTWLAGLYLAWAGHWFSSSWLHGKLLLVVLLSGVHGFFSRC
                     VKDFAADRNLRSQKFYRIINEVPTVLMIGAVVLVVVKPF"
     gene            11813..12649
                     /locus_tag="VGA15_06740"
     CDS             11813..12649
                     /locus_tag="VGA15_06740"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX9587429.1"
                     /translation="MPTTGNYFHLHLVSDSTGETLITVARAVAAQYANVTPVEHVYPL
                     VRSQKQLDRVLSEIEEAPGIVLFTLLEKDLVGRLEAKCQQINIPSLSIIGPVMHLFQA
                     YLGAATTGRVGAQHTLNAEYFKRIDALNYTMMHDDGQHVEGLEEADVVLVGVSRTSKT
                     PTSIYLANRGIRTANVPLVPGIPLPHQLETLTKPLVVSLHATPERLIQVRQNRLLSMG
                     DRDNDTYIDRQAVADEVAFARRLSAKFNWAQLDVTRRSIEETAAAVMKLFTDRQRQRL
                     AE"
     gene            12646..>12807
                     /locus_tag="VGA15_06745"
     CDS             12646..>12807
                     /locus_tag="VGA15_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX9587430.1"
                     /translation="MTLWRGKHPLVLASQSLTRQALLANAGIDFEAVAAELDERAVQH
                     ASGLLAPGDI"
BASE COUNT         2390 a         4035 c         3954 g         2428 t
ORIGIN      
        1 agctgcattt caacgtctgc cttgcctaca tccgggaatt ttccaatacg ctgggcgagg
       61 tgctgggccg cgaggggcgg ccgccgccgg atctcgcctg ggccgtgggc gaggccgcgg
      121 tcatcctggt tcaattggtc gagccgatga tgccgcatct ggcggaagag tgctggaccg
      181 tgctggggca gccgggcctg atttcggagg cccaatggcc ccaaatcgaa cgcgatttgc
      241 tggttgaaga caccgtgacg ctggtggtcc aggtcaacgg caagaaacgc ggcgaagtca
      301 cagtggcccg caatgcgcaa aatccggaaa ttgaggctgc cgttttggcc ctcgatgcgg
      361 taaaacaggc gctcggcggc aaagccgtcc gcaaggtcat tattgttccc atgaggatcg
      421 tgaatgtcgt tggctagcat ccgtatcgcc gctcggcttc tggtcgtggc cggattggcg
      481 gcgctgaccg ccggctgctt ccagccgatg tacgccgaac atactgatgg cacccccggc
      541 ctgcgcgaaa agctgatggg cgtggaagtc ccgcccgtgg acaagcctaa cgcctcgcaa
      601 gaagcgcgga tccaggtgga aatccgcaat gcgctggctt tcaaacttta cggcaacgcc
      661 accggcatgc cgccgaccca ccggctggta ctgaagttta ccaccagccg ttcgtcgctg
      721 atgatcgacc cagctaccgc gttgccgaca agcgaaaact tcggcatcga cgcgcagtac
      781 aatctggtcg aaatcgcctc gaacaaatcg gtcatgaccg gagccacatt ctcacgcgtg
      841 tcctatgaca tcccgggcca gctgcagcgc ttcgcccgat cgcgcgcctt ccgggatgcc
      901 gaggatcgcg ccgcccagga gatcgccgaa agaatccaga cccggctggc gtcgttcttc
      961 gtcgccggca cctgatccct tccgtcattc cggggcgcgc gaagcgcgaa cccggaatct
     1021 cgatcaatta catctggatt ccgggtccac gcgaaggcgc gtgtcccgga atgacaacga
     1081 aagtctgacc gtggtcgcgc ttcgcggaaa agacatcgac gcctttctcg cccggcccga
     1141 tccgggacgt cccattatcc tgctctacgg tcccgattcg ggcctcgtgc gtgaacgcgc
     1201 cgaggcgctg ctggcctccg cggtcgacga tcccaacgat ccattttcgc tggtccggct
     1261 ggacggcgac gaactctcgg ccgagccgtc gcggctggtc gatgaagcga tgacggtgcc
     1321 actgttcggc ggccgccgcg ccattcgggt ccgggccggc tcgcgaagtt tcgccagcgg
     1381 cgtcgatacg ctcgcggact cccccctgaa ggattgccgc atcgtgatcg aggccggtga
     1441 gctgcgcccg gaatcgccgc tgcgcaaagc ctgcgagcgc gccaagaccg ccgtggccat
     1501 cggctgctat cccgacggcg aacgcgatct ggcgaaattg atcgacgatg agctgcgggt
     1561 ctccaatttg aagatcgcgc cggacgcgcg cgcggtgctg atggcgctgc tcggcggcga
     1621 ccgccaggcc tcgcgcaacg aattacgcaa gctcacgctt tatgcgcatg gcgcaagcga
     1681 ggtcatgctc gaggacgtga tggcggtggt cgccgacgct tccgagctga agatcgatcc
     1741 gatcgtggac ggcgcgtttg ccggaaggcc ggatctggtc gaaaccgaat ttgccaaggc
     1801 catgatcgcc ggcacctatc cgggcatgat catctccgct gcgcagcgcc aggcggcctg
     1861 gctgcataaa tcggctctcg cggttgccga aggcacgccg ctgtcgacgt tgctcgacag
     1921 cggctttccg cggctgcact tctcgcgcaa gggcaatgtc gaaaccgcgc tgcgcaattt
     1981 cagtgcggcg cgactggtgc tgatcataga gcaactcgcg accgccgcgc tggaagtgcg
     2041 caagcaggcg gcgctggcgg cggtgatcgc gcagcggacc ctgctgtcga tcgcggtgaa
     2101 tgccaggcgg cgggggtgag ctcttctccc tcgccccgct ctgcgcgggg tcgaaacgag
     2161 cgaagctcgc tcttggaggg tcggggtgag gggctctccg acccgcgaat gctgacgatg
     2221 aagttggcgc agtgcctggc ccctcacccg ccgcgcgaag agcgcggcga cctctccccg
     2281 cgcagagcgg ggagaggcaa gacagtcagc ttcccgtttc cagccgtcgg acaatctcgt
     2341 cgagctggtc gaggtcaccg tagcggacgt gcacggtgcc accggttccg cggtgatcga
     2401 cgttgaccgc cagcccaagc gcgtcgctga cccgcttctc cagcgcgacc gtattgggat
     2461 ccttcagctt gccgccgcgc gccttctgcg gcttgcgctc gggcacgccc tcgacatgcg
     2521 ccagcgcctc ggcctggcga acattgagtc cttcctcgac gatacgcttg gcggcggccg
     2581 ccgggtcggg cacgccgatc agcgcgcggg cgtggccggc cgacagctgg cccaacgcga
     2641 tgtaggcctg cacctcggcc ggcaatttcg tcagccgcat catgttggcg acgtggctgc
     2701 ggctcttgcc gacgatcttg gcaatctcgt cctggctgcg tttgaattcg ttggcgagcg
     2761 cgtgatagcc ctgcgcctct tccataccgt tgaggtcttc gcgctgcacg ttttcgatga
     2821 tcgcgatctc gagcgcgtcg ctgtcgctga catcgaccgg cacgatcggc acctcgtgca
     2881 ggccgacgat ctgcgacgcg cgccagcggc gctcgccggc gatgatctcg aaacggtcct
     2941 gcgcgccctt gaccggacgc accacgatcg gctggatcac cccgtgctgc ctgatcgagt
     3001 cggcgagttc gccgagttcg gcatcggaaa aatcccggcg cggattgcgc ggattgggct
     3061 tgaggaattc aatcggcacc ttgcgctggg tgcgcggccg atcctgatgc ttggcttcgc
     3121 cgccgacatc tccgatcaga cttgcaagac cgcggcccaa tcgcgaacgc gcatcgtcgg
     3181 ccatcgccag ctcccttgga ttcaatttga gtgctccgga ttggacgcgt ctgtttctca
     3241 accatccttc gaggctcggg gcgctgcccc tcgcacctca ggatgacgcc tgcgatggtt
     3301 gccgtcaccc tgaggtgcgc gcgctagcgc gcctcgaagg gcgacggcca tctcaatgcg
     3361 ggcgcaggtc gcgctcgcgc tggatcactt ccgttgcgag cttgaggtag gcttcgctgc
     3421 cgacgcattt gagatcgtac accagcaccg gcttgccgta ggacggcgcc tcggagatgc
     3481 ggacgttgcg cgggatcatg gtgttgtaga ccttgttgcc catgaactgc ctgacgtcgg
     3541 cgacgacctg gttcgacagg ttgttgcggg agtcgaacat ggtcagcacg atgccgtgaa
     3601 tcgtcaggtt gggattgagc gtcgagcgca cctgctccac cgtctgcagt aattgcgaca
     3661 gaccctccag cgcgaagaac tcacactgca acggcaccag gatcgagtcc gaggccgcca
     3721 tcgcattgac cgtgagaaga ttgagcgacg gcggacaatc gaccagcaca taggtgtaat
     3781 cggagtccgg cgtgacgctg gtgttcaatc ccgcgatggc attgcgcagc cgaaacgcac
     3841 ggtcgcgcgt cgcgcccagc tcaagttcga gacctgaaag atccatcgtc gaggtcgcga
     3901 tatgcagccg cggcaccgcg gtcggcacga tcgcgtcgcg caacggcgcc tctccgatca
     3961 gcacgtcgta ggttgagcag ctgcggttgc ggcggtcgat accgagacca gtggaagcat
     4021 tgccctgcgg atcgagatcg acgatcagca cattctcgcc gatggcggcc agtgccgtgc
     4081 cgagattgat cgcggtcgtg gtcttgccga cgccgccctt ctggttggcc agcgccagga
     4141 tgcgcggatg gtgcgccggg cttgggacct tatcttcttg ataaacctca tcaattacgg
     4201 tcatgcgcgg gtgccatgtg ctgtcgcgga cgggttacgc cgctcgatat ggtcgagctc
     4261 gacaatccag ccctgtccgc cggtgcggct ggaatgcaat tgcggcttga tattccaata
     4321 tttggtggct tccgtcaatt cagcctctac atcttggccc ttaagaaaca gggctttcgc
     4381 accccttttt accagcggtt ccgcgaagcg gatcagctgg tgtaacgggg ccaccgcccg
     4441 cgccgtgacg caatcgatct ggccggcgat tctatccaca ctatccccga tttccgccaa
     4501 atgcacgatc ccaggcgacg ttgaaatgcg aagcgcctca cgcaggaagg cggccttctt
     4561 ggcattgcgc tcgaccagat gcaccatcgc gcccgatgtt tccgccagtg cgcaggccag
     4621 cactacaccg ggaaaccctc cgccgctgcc cagatcgacc cagacttttg cagacggagc
     4681 gagcgtcaac agctgcagtg aatcggcgat atggcgggtc cacaaatttg gaagcgtcga
     4741 cggcgccacg agattggtct tcgcctgcca ctccaatagc aggtcgacgt aacgatccag
     4801 ccgcgcttct gtttcatgtg aaacgggagt tagcgcgagg gcggcagcct tgtcggcctc
     4861 tgacccagtc gaagctggag cctgatgcga tcgttgagcc atgagatgaa acgaaccggt
     4921 ggtcgcgggt gaccgctctt ccgaaccgcc aaccaggatg gatgtgatac gcgcccgcgc
     4981 gccagctatc cataaatttt gaacagcctg ctcgcgccaa ccctccgccg caagcgggag
     5041 agggagctct ccgagtcgtg acgcgtagcg tcggatcgtg acgacgagga atgaacaatc
     5101 gcaagcgagc tgaaactcac ctatcccgtc ggatgccgtc ttctctcttt ttccttcccg
     5161 tttgtcggga gagagttcgt gacgtctcgt ggcagacacc ttcctgcaaa acgcacgctg
     5221 ctcatatcgg acggcattct ctctcccgct tgcgggggct gcttgccacg caacgaagga
     5281 aatcgggtta agaaaactgg ccaccccgtt tcacgtgaaa cgaattacgc cgtcgccacc
     5341 gaatgctttc gtcgtgcctc ccgtcgcaga taggccgcca ggatgccgag cgcagccggc
     5401 gtcaaaccgt cgagccggcc cgcctgcccc accgtgcggg gacgcgcggc ttcgagcttt
     5461 gagcgggcct cgttggatag tccgggcaca tcggtatagt cgacatcggt caggatcaaa
     5521 ccctcgtcgc ggcggaaggc atcgacatcg gcggtctggc gcttgagata gacatcgtac
     5581 ttggcatcga tctcgagatg gatcgcgatc gatgagccaa tggccgacag gtccggccag
     5641 atctcccgca gctctttcca gccaatctcg ggataggcca gcagctcgaa cgccgagcga
     5701 cgatggccat ccttgttgag caccagccca tgtcgcgcgg cttcgttcgg tgtgagcgtc
     5761 agcgacttcg ccaatgtctt cgccgcatcg agcgccgcca tcttggtgcc gtgacggatc
     5821 gcacgagcct gccctacgca gcccagcgca atgcccttgt cggtcaggcg ctgatcggca
     5881 ttgtcggccc gcaaggtcag ccggtactcg gcccgcgagg taaacatccg gtacggctca
     5941 gtaatgccgc gcgtcaccag atcgtcgatc atgacgccga ggtagccatc ggcgcgatcg
     6001 aacacgattg cgtcggtccc gccggcggtc aatgccgcgt taaggccggc gacgagtccc
     6061 tgcgccgccg cctcttcgta accggtggtg ccgttgatct gcccggccag gaacagaccc
     6121 agcagccgct tggtctgcag ggtcggatcg agctcgcggg gatcgacgtg gtcgtattcg
     6181 atggcatagc ccggccggac catccgcacc cgctcgagcc cgggaatcgt cgccaggatt
     6241 gcgagctgca cctcttccgg cagcgaagtc gagataccgt tgggatagac cgtggtgtcg
     6301 tcgagccctt ccggctcgag gaaaatctga tgtccatctc gatcgccgaa gcggacgatc
     6361 ttgtcctcga tcgagggaca atagcgcgga ccgctgctcg tgatctggcc ggagtacatt
     6421 ggcgagcgat gtacattggc ccggatcacg tcatgggtcg ctggcgtggt ccgggtgatg
     6481 ccgcactgga tctgcggcgt cgtgatccga tcggtcatca ccgagaacgg ctccggtgga
     6541 tcgtcgcccg gctgcatctc gaccgcggac cagtcgatgg tgcggccatc cagccgcggt
     6601 ggcgttccag tcttcagccg tccgagcgtg aagccggcac gctcgaagga cttcgagagt
     6661 cccatcgccg gtgcttcgtc gacgcggccg gccggccaat tcctctcgcc gagatggatc
     6721 agtccacgca gaaaggtccc ggtggtgatg acaacggcac cggctgaaag ctcgcttcta
     6781 tcggccacgc ggatgccagt gactcgaccg ttcgagacga gcagctcgtc cgcctcgcct
     6841 tcgatcacgc ggaggttcgc ggtttcaagg atggcggcct gcatcgccgc ggcgtagagc
     6901 ttgcggtccg cctgggcgcg cggaccacgc actgccggac ccttgcggcg attgagcatc
     6961 cgaaactgga tccccgcgac atcggccacg cggcccatca ggccgtccag cgcatcgacc
     7021 tcacggacca gatggccctt gccgagacca ccgatcgcgg gattgcagga catggccccg
     7081 atggtcgcaa accggtgggt caccagcgcg gttttcgcac ccatgcgggc ggcggcgctg
     7141 gcggcttcgc agccggcgtg gcctcctcca atgacaataa cgtcgaacga ttccggtcgg
     7201 gcgatcatgc cggagctttt atcgcggggt gacgaatctc ggaagccgta agtttatcgc
     7261 cggatgtttc acgtgaaaca tcccgccaaa catccggctg agagagggat ggtcttgatg
     7321 gaatcggtcg gtcagcggat tcagttcacc tctcccgctt gcggaggaga gagtagtccg
     7381 cttttggcgg ccgtccttaa ataagaacgc cgaagcgagg cttcggccac ggcgctcgat
     7441 ggagcgcggc ggtgggggaa ctgcctgcca agcaaaatcc actgccgatg cggcaccccc
     7501 accccagccc ttcagagcga gcttcgctcg tctcgacccc gcaagcggga gagggagcgc
     7561 acttccgttg ttgctgccgc tcaatcaaaa ctcatctcgc ttcggagaag gcgtgcgcga
     7621 taatctttat ctttatattt gtgtttcacg tgaaacggtt tcacgcgaaa cgtttcgtta
     7681 aggttaataa gacaaactca cttacctata cagaattcac ggaagatcac gtccaggaca
     7741 tcctcgacgt cgacccgtcc gagcagtctg ccgagggacc gagccgccat ccgcaaatcc
     7801 tccgccgcca gttcctcacc ttcaggaatc accgacagac tgcgctgcaa cgacacggcc
     7861 gtctcctgca gaagtttgcg ctgccgctcc cgactgatca ggccgccctc ccccgcacca
     7921 aaataatttt gcgcgaatac gatcaacgcg gcgatcaatt cctgaatgcc gtcacccccg
     7981 ctggccgaga tttcaaagtc ggtgtggcgc tgtgtctgcc ccaagacgcc ggcgggctgt
     8041 ccgggattca gcgcgtcgat tcccagcacc gcggcatctg atccgaccgc gtcgagatcg
     8101 atcttgttgc gcaccaccca cgccggcgcg gcaccttgct ccaaactttt ttccaaattt
     8161 ttttcgtcgt gcgcatcgac cagccacagc acgaggtctg cttccgccgc gcgcgcccgg
     8221 gcgcggcgca cgccttcctg ctccacagga tcgtcggtct cgcggatgcc ggcggtatcg
     8281 atcaccgtca ccggatagcc gtcgaggtcg agctgcactt cgatgacgtc acgcgtggtg
     8341 ccggcatgcg gcgagacgat cgccacctcg cgccgcgcca gctgattcat cagcgtcgac
     8401 ttgccgacgt tcggcggtcc tgcgatcgcg accaccagtc cctcgcgcag ccgttcgccg
     8461 tgccctcgcg ccgccagcac ctgccggatt tcaccgagca gcgctttgat ttttttcagc
     8521 gccggcgcga tcagctccgc gggcacgtcg gcttcgtcgg agaaatcgat ccccgcttcg
     8581 atcagcgccg aggcctcgat gatctgcgcg cgccagtcgc gcgccctgtc gccgagcaga
     8641 cccttcagct gactcagcgc ctggcggcgt tgccgatcgg tgtcggcgtg gatcagatcg
     8701 tcgagaccct cggcctccgt gagatcgagc ttgccgttct caaaggcgcg gcgggtaaat
     8761 tcgcccggct ccgccgcgcg cagattgtcg aaatccgaga gtgctgcgaa cagcgccgcg
     8821 agaacggcgc gaccgccatg gacgtgaaac tcggcgacgt cttctccggt cgcgcttgcc
     8881 ggtgcgggaa accagaggat caccgcatcg tcgatcggct ggcgattgcg atcgcgcagc
     8941 agcgcacgcg tggccatccg cggcgctgga atcttgccgg cgagcagggt cagcgccgcc
     9001 ccggcccgcg gtccggatac acgtgcaatg gagatcgcac tcggcggccg acccgatgac
     9061 agtgcgaaga tggtttggtc gcgcggatgc atcgcctatt tgctccaggg cgcgcttgcg
     9121 aagccaacga aaatttgcgc ggcgcggggc tgtcacacac cccaggccgg tcggccatag
     9181 gcctcgacca gggtcgcgga ttcaagtgcg ggatgcggcg ttgtcgagcg gcgccgctgc
     9241 gtgtctcaat aaaacgctca aacagcgctc aggaacatag cacgaacacc tatacaagct
     9301 agccgcgcgg gcgctttgaa ccaggcacaa aaaaagggcg cctccgctcg cgcgaggacg
     9361 ccccgtttca aacccggcga gccagctcag gtattcatcg actcgaagaa ctccgagttg
     9421 ttcttggtgt tgcgcagctt gtcgagcagg aagtcgatcg cgtccatcgt gcccatcgga
     9481 ttgaggatcc ggcgcagcac gtacatcttc ttcaggagtt gcggatcggt gatcagctct
     9541 tccttgcggg tgccggagcg cgagatgtcg atcgcgggga aggtgcgctt gtccgaaacc
     9601 ttgcgatcga gaatcagttc ggaattgccg gtgcccttga attcttcaaa gatcacttcg
     9661 tccatgcggc tgccggtatc gaccagcgcg gtcgcgatga tggtgagcga accaccctcc
     9721 tcgatgttgc gcgcggcgcc gaagaaacgc tttggccgct gcagcgcgtt ggcgtcgaca
     9781 ccgccggtca gcaccttgcc ggatgacggc accacggtgt tgtaggcgcg tcccaaacgg
     9841 gtaatcgagt ccagcaggat gacaacgtcg cggccgtgct cgaccagccg cttggctttc
     9901 tcgatcacca tctcggcgac ctggacgtga cgcaccgccg gctcgtcgaa cgtcgatgag
     9961 acgacttcgc ccttcaccga gcgctgcatg tccgtgactt cttccgggcg ctcgtcgatc
    10021 aggagcacga tcagatagca ctccggatga ttggcggtga tcgagtgcgc gatgttctgc
    10081 atcagcacgg tcttgccggt gcgcggcggc gccacgatca gggcgcgctg gcccttgccg
    10141 atcggcgcga cgatgtcgat caccctcgca gaaagatcct ttctggttgg gtcttccagc
    10201 tcgagccgga agcgctggtc gggaaacagc ggcgtcagat tgtcgaagtt gaccttgtgt
    10261 ttggatttct ccgggtcttc aaaattcagc gtgttgacct tcagcagcgc aaaatagcgc
    10321 tcgccttctt tcgggctgcg aatgtggcct tcgatggtgt cgccggtgcg aagcccgaag
    10381 cggcggatct gcgagggcga gacatagatg tcgtcgggcc ccggcagata attggcgtcc
    10441 ggcgagcgca agaatccaaa tccgtcggag agaacctcga cgacgccttc gccgataatg
    10501 tcggtttcct ggatcgcgag ttgtttgagg atggcgaaca tcagctcctg cttgcgcatc
    10561 gtgctggcgt tttcgacccc tttctcttcg gcgaacgaaa cgagttcggc cggcgtctga
    10621 gccttgaggt cttgaagttt catttcccgc attgggatgg tcctgtggaa taactaggga
    10681 gggatcgcga ggtggcttta agaaaatgcg gacgcccgaa aaggaagaag gtccgcaggt
    10741 ctgagcgagg aaagggccgg agaggcttca aacctgatgc ggcggccggt tcaacgagga
    10801 gaccggaacg caaccacatc cgcctgcgtg gggtgggatt tcaacaatat agaaaatcga
    10861 tcagctttct gcaaggagac ttggcaggca gcgcatggac ggcggtgcct gttaaaacgg
    10921 cttaaccacg accaggacca cagccccgat catgaggacc gtaggtactt cattgataat
    10981 acgatagaat ttctggctac gcaggttgcg gtcggcggcg aaatccttga cgcagcggga
    11041 aaagaaaccg tggacacccg atagcaacac caccagcaga agcttgccat gcagccagct
    11101 cgatgaaaac caatggccgg cccaggcgag gtaaagcccg gccagccagg tcacgatcat
    11161 cgccgggttg atgatcgccc tcagcaatcg ccgttccatc accttgaagg tctcggattg
    11221 cttcgatccg gcttccgact cgcagtgata aacgaacagc cgcggcagat acagcatgcc
    11281 cgccatccac gagatgacgg cgatcacatg cagcgccttg atccattcat acagcatgga
    11341 attctaactc gcccgcgatg gtgtggaatt tccagccgat cagcgtggca acgtttcgac
    11401 gaatgatcgc gttgaaaagc atgtcgccta aagacccgaa tggaagcctt tccgcaagcg
    11461 gcacttttct aaaacaataa ttttagaatc ttaggtttga gtctaagtag cggtggatta
    11521 ggcccacgca aaactgtccc gcaattctcc gggcttgttc acatccatca acaatttctc
    11581 gggtcggacg gtgatgtcga taacgtcacg ctcgatcaag cacttgcgga tcgctgtaag
    11641 ccgattatca agagacaagt ccacagaacc gcactatcat cgccgctgtg gactggactt
    11701 atccttattc gagccctgtg aagaaaaaac cgggttgtcc ccgatggatc ggccgtcgca
    11761 tgattctcgc ggaaaagctc cacagcgatt cacaggatac acaggggttc ccgtgcccac
    11821 gaccggcaac tattttcatc tgcacctggt ttccgattcg accggtgaga cgctgatcac
    11881 ggtggcgcgc gcggtggctg cgcaatatgc caatgtgacg ccggtcgaac atgtctaccc
    11941 gctggtgcgg agccagaagc agctcgatcg ggtgctgagc gaaatcgagg aagcgcccgg
    12001 catcgtgctg ttcaccttgc tggaaaaaga tctggtcggc cggctggaag ccaagtgcca
    12061 gcagatcaac atacccagcc tttcgatcat cgggccggtc atgcacttgt ttcaggccta
    12121 tctcggtgcg gccacgacgg ggcgcgtcgg cgcacagcac acgctgaatg cggaatactt
    12181 caagcgcatc gacgcgctga actacacgat gatgcacgat gacggtcagc acgtcgaagg
    12241 gctggaagag gccgacgtgg ttctggtcgg cgtgtcccgg acatcgaaaa ccccgacctc
    12301 gatctaccta gccaaccgcg gcatccgcac ggcaaacgtt ccgctggtgc cgggcatccc
    12361 gcttccgcat caactggaga ccctgacgaa gccactggtg gttagcctcc atgcgacgcc
    12421 cgagcggctg atccaggttc ggcagaatcg gctattgagc atgggcgatc gggacaacga
    12481 cacctatatc gaccgtcagg ccgttgcgga tgaagtcgca ttcgcgcgcc gattaagcgc
    12541 caagttcaac tgggcgcagc ttgacgtcac acggcgatcg atcgaggaga ccgccgccgc
    12601 cgtcatgaaa ctgttcaccg acaggcagcg gcaacggctt gccgaatgac cctctggcgt
    12661 ggaaagcatc cgttggttct cgcctcgcaa agcctgacgc ggcaggcgct gctggccaat
    12721 gcgggcatcg acttcgaagc ggtggcggcc gagctcgatg agcgggccgt gcagcacgct
    12781 tccggccttt tggcacctgg cgacata
//
LOCUS       DASWGM010000221         3689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2514009,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000221 DASWGM010000000
VERSION     DASWGM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2514009"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1535
                     /locus_tag="VGA15_06750"
     CDS             312..1535
                     /locus_tag="VGA15_06750"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9587431.1"
                     /translation="MTAQTAVKPALAPQTPPLPQAKPESLGLSSIRLQRMSDAFKREI
                     DKGTVPGVTMMVARRGQIGWFEALGKQSPAASTPMAQNTIFRIFSMTKPIVSIGIMML
                     LEDGHLLLSDPVAKFIPEFSDQKVGVENNGKLELVPVKRPMTVQDLLRHTSGITYEHT
                     GNGLVQQLYKQSRLRSRQITNAEHATLVASLPLMCQPGAEWNYSRSTDILGRIIEVVS
                     GNTLSAFLTERILAPLQMAETAFHTGEENAGRLAEPFPTDPWTGEKVQLFNMLEKPVM
                     ESGGGGLVSTTMDYARFSQMLLNGGVLDGNRIVGRKTLQLMASDHLGPKVKVDSFLMP
                     AGHGFGLGFAVRTHQGMAPFPGSLGQFFWSGLPGTFFWVDPAEDMFAVFMMQGPGQRE
                     YFRSLVRNLVYAAVE"
     gene            complement(1603..2370)
                     /locus_tag="VGA15_06755"
     CDS             complement(1603..2370)
                     /locus_tag="VGA15_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9587432.1"
                     /translation="MTLFDMSGKVAVITGSSRGIGRAIAERMAEHGAKVVISSRKQQV
                     CDEVAKAINDKAGKQVALAVAANISTKDDLKHLVEETNRVFGRIDTLVCNAASNPYYG
                     PQAGISDDQFRKILDNNIVANHWLISLVAPQMIERKDGSITIISSIGGLKGSTVLGAY
                     AISKAADMQLARNLACEYGKHNIRVNCIAPGLIKTDFAKALWDNPETLKASTARSPLL
                     RIGEPDEIAGAAVFLASAAGTFMTGQTIVIDGGATIS"
     gene            complement(2475..3209)
                     /locus_tag="VGA15_06760"
     CDS             complement(2475..3209)
                     /locus_tag="VGA15_06760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9587433.1"
                     /translation="MSNPAKPSVAQQSVPNPVVATRWWWVRHAPVREDGGCIYGQKDL
                     GCDTSDRVVFEAVGKILPRNAVWYASNLKRTHQTADAIWAAGFPKPLAMAREAALAEQ
                     HLGEWQGMNRAAFIASRPIGSHWFADIDEPAPGGESFMDLYNRVRGAIERISAEQAGK
                     DVIAVAHGGTIKAAIGLALGNQPEQGLAFDIDNCSVTRLDHFASAGQSRWRLPMVNQQ
                     PWIADASHNAMHQPAGPEIASAGKLA"
     gene            3349..>3689
                     /locus_tag="VGA15_06765"
     CDS             3349..>3689
                     /locus_tag="VGA15_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9587434.1"
                     /translation="MGRLEGKSVIITGAGSGIGRAASLLFTKEGAKLIAVDRAEGVKE
                     TVAQVREAGGTAQAVMADAGSERDVMAFIDKAVSAYGRLDAIWANAGVSGGLVPIAEQ
                     TVEHWQEILRI"
BASE COUNT          683 a         1170 c         1142 g          694 t
ORIGIN      
        1 tgcgaccccc ggttttggag accggtgctc taccaattga gctacactcc tacagggaac
       61 ctgcgtcgcc gccgatccgc gccgtttcaa gcataggcga cgccccgatt gcaagggcga
      121 agccgtaaac cgcgggtgat ccggcggcct cagcttgcac gatagcccag tcgtctatgc
      181 ggcagcaaac cggcgggtgc tctttgcgga aaacgtgctg ttcaaccggg gtgttctgcg
      241 ccagaattcc tggcgccccg ccgatggacg ctgcccatcc ggcaaatgac gagagcaaga
      301 cgggagagac catgaccgcg cagaccgccg taaaacccgc cctcgccccg cagacgccgc
      361 cgcttcccca ggcaaaaccg gaatcgctgg ggctctcgtc gatccggctg cagcggatgt
      421 cggatgcgtt caagcgcgag atcgacaagg gaaccgtccc gggcgtgacc atgatggtgg
      481 cgcggcgcgg tcagatcggc tggttcgaag cgctcggcaa gcaaagcccg gccgcctcca
      541 cgccgatggc ccagaacacg atcttccgca tcttctcgat gaccaagccg atcgtctcga
      601 tcggcatcat gatgctgctc gaggatgggc atctcctgct cagcgacccc gtggcgaaat
      661 tcattccgga attttccgat cagaaggtcg gcgtcgagaa caacggcaag cttgagctcg
      721 tgccggtgaa gcggccgatg accgttcagg atctgttgcg ccacacctca ggcatcacct
      781 acgaacacac cggtaacggc ctggtgcagc agctgtataa gcaatcgagg ctgcgcagcc
      841 gccagatcac caatgccgaa cacgcaacgc tggtcgcaag cctgccgctg atgtgccagc
      901 cgggcgcgga atggaattac agccgctcca ccgacattct cggccgcatc atcgaggttg
      961 tttccgggaa cactttgagc gcgttcctga ccgaacgcat tcttgcgccg ctgcagatgg
     1021 ccgagacggc attccatacc ggtgaggaga acgccggccg cctcgctgag ccgttcccga
     1081 ccgatccctg gaccggcgag aaagttcaac tcttcaacat gctggaaaag ccagtgatgg
     1141 aatccggcgg cggcggcctg gtttcgacca ccatggacta tgcgcggttc agccagatgc
     1201 tgttgaacgg tggcgtgctc gacggcaaca ggatcgtcgg ccgcaagaca ctgcaactga
     1261 tggcgtccga tcatctcggc cccaaggtca aggtcgattc tttcttgatg ccggccggtc
     1321 acggcttcgg tctcggcttc gcggtccgca cccatcaggg catggcgccg tttccaggat
     1381 cgctcggcca gttcttctgg agcggactgc ccggaacctt cttctgggtc gatccggctg
     1441 aagacatgtt cgcggtgttc atgatgcagg gcccgggcca gcgcgaatat ttccgctcgc
     1501 tggtgcgaaa cctggtttac gcagcggtgg agtgaatgcg tctgcgcgcg aaatagatta
     1561 cgtcgtcccg gcgaacgccg ggacgacgag agttttgcca tctcaactaa tcgtagcacc
     1621 gccgtcgatg acgatggtct gcccggtcat gaacgtcccc gcagctgagg ccaagaacac
     1681 cgccgcaccc gcgatttcat cgggctcgcc gatgcgcagc agcggtgagc gggcggtcga
     1741 tgccttcaac gtctccggat tgtcccacaa ggccttggca aaatcggtct tgatcaggcc
     1801 cggcgcgatg cagttcacgc gaatgttgtg cttgccgtat tcgcaggcca gattgcgcgc
     1861 gagctgcatg tcggcggctt tcgaaatcgc gtaggcgccg agcaccgtcg aacccttgag
     1921 gccgccgatc gaggagatga tggtgatcga gccatccttg cgctcgatca tctgcggcgc
     1981 caccaggctg atcagccagt gattggcgac gatgttgttg tccaggatct tgcgaaactg
     2041 atcgtcggaa attcccgcct gcggcccgta atagggattc gacgccgcgt tgcagaccag
     2101 cgtgtcgatc ctgccgaaca cgcggttggt ttcctccacg aggtgtttca ggtcgtcctt
     2161 ggtcgagatg ttcgcggcca ccgcgagcgc aacctgcttg ccggccttgt cgttgatcgc
     2221 cttggccacc tcatcgcaga cctgctgctt gcgcgacgag atcaccacct tggcgccatg
     2281 ctcggccatc cgttcggcga tggcgcggcc gatgccgcgc gaggaaccgg tgatgacggc
     2341 gacttttccc gacatatcaa acaaggtcat gtttcactct cccaaatatt gatccggcgg
     2401 cggatgcgcc ggtttcactt caaatccgag ctaagcagcg ttcgcgcggt cagacaactg
     2461 catgaaggct gacttcaggc cagtttgccg gcgcttgcga tttccggtcc cgccggctgg
     2521 tgcatggcgt tgtgcgaggc gtccgcgatc cacggctgct ggttgaccat cggcagccgc
     2581 cagcggctct ggccggcact tgcgaaatga tcgagccgcg tcaccgagca attgtcgatg
     2641 tcgaaggcga ggccttgttc gggctggttg ccgagcgcga gcccgatcgc ggccttgatg
     2701 gtgccgccat gcgcgaccgc aatgacgtcc ttgccggctt gttcggcgct gatccgctcg
     2761 atcgcgccgc gcacacggtt gtagagatcc atgaaactct cgccgcccgg cgcgggctcg
     2821 tcgatatcag cgaaccagtg gctgccgatc ggccggctgg cgataaatgc cgcgcggttc
     2881 attccctgcc attcgccaag atgctgctcg gccagcgccg cctcgcgcgc catcgccaga
     2941 ggcttgggaa atcccgccgc ccagatcgcg tcggcggtct gatgcgtccg cttcagattg
     3001 ctcgcatacc acaccgcgtt acgcggcagg attttcccga ccgcttcgaa taccacgcgg
     3061 tcgctggtgt cgcagccgag gtccttctgc ccatagatgc agccgccatc ttcccgaacc
     3121 ggcgcgtgac gcacccacca ccaccttgtc gcgaccacgg ggttgggaac cgactgctgt
     3181 gcgacggatg gtttggctgg attggacatc gcaaggaccc ttcaatactg tcgcgtgtcc
     3241 tacgctagag catgcaccgc ctcaagtgac ttcggcgttt gaaatcttgt ttgaaattcc
     3301 gcaaggcggc aagcgccgat gcgtagagaa gctaacaggg agaaactcat gggccgcctc
     3361 gaaggcaaat ccgtcatcat caccggcgcc ggcagcggca tcgggcgcgc ggcatcgctg
     3421 ctgttcacca aggaaggcgc caagctgatc gcggtcgatc gcgccgaagg cgtcaaggag
     3481 acggtcgcgc aggtgcgcga ggccggcggc acggcacagg ccgtcatggc cgacgccggt
     3541 tcggaaagag acgtgatggc tttcatcgac aaagcggttt cggcttacgg aaggctcgac
     3601 gcgatctggg ccaatgccgg cgtcagcggc ggcctggtgc cgatcgccga gcagaccgtg
     3661 gaacactggc aggaaatcct gcgcatcaa
//
LOCUS       DASWGM010000222         2871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1262089,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000222 DASWGM010000000
VERSION     DASWGM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262089"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /gene="sucA"
                     /locus_tag="VGA15_06770"
     CDS             complement(<1..1003)
                     /gene="sucA"
                     /locus_tag="VGA15_06770"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490619.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX9587435.1"
                     /translation="MSRQDANAAFALSSFLQGTNAPYIDDIYARYERDSASVDAEWQE
                     FFKSLKDRPADVRKNAEGPSWARNNWPLAPRDELTSALDGNWAEVEKTVGIKIAAKAQ
                     TKGAEVTAADIHQATRDSVRALMLIRAYRMRGHFHARLDPLGIEPPRDREELDPRSYG
                     FTEGDFDRKIFLDHVLGLEYATLREILTICERTYCQTLGVEFMHISNAAQKAWIQERI
                     EGPDKEISFTREGRRAILTKLVEAEGFEKFCDLKFTGTKRFGLDGAESLIPALEQIIK
                     RGGNLGVKEIVFGMPHRGRLNVLTQVMGKPHRALFHEFKGGSVNPDAVEGSGDVKYHL
                     "
     gene            complement(1138..2022)
                     /gene="sucD"
                     /locus_tag="VGA15_06775"
     CDS             complement(1138..2022)
                     /gene="sucD"
                     /locus_tag="VGA15_06775"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9587436.1"
                     /translation="MSILIDKNTKVICQGFTGKNGTFHSEAAIAYGTKMVGGTSPGKG
                     GAVHLGLPVFDTVAEAKEKTGANASVIYVPPPGAADAICEAIDAEIPLIVCITEGIPV
                     LDMVRVKRSLSGSKSRLIGPNCPGVMTAGECKIGIMPANIFKPGNVGIVSRSGTLTYE
                     AVFQTTQEGLGQTTAVGIGGDPVKGTEFIDMLEMFLADPKTTSIVMIGEIGGSAEEDA
                     AQFIKDEAKRGRKKPMVGFIAGVTAPPGRRMGHAGAIISGGKGDAGSKTAAMEAAGIA
                     VSPSPARLGKTLVEKLKH"
     gene            complement(2058..2537)
                     /locus_tag="VGA15_06780"
     CDS             complement(2058..2537)
                     /locus_tag="VGA15_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX9587437.1"
                     /translation="MPEDHLAAPSPLEEHRKLAVFAGEWNGEEVVFPSRWTAGGPATS
                     HVIARMDLNGFYLIQDTRQMRDGKETFATHGVFTYDREDRHYKLFWHDSLGYYSPAPA
                     SGGWTGKTLIVVRGSLRGNARHVYEVVDDNTYTMKIQFSPDAEGWADVLTGVYRRIP"
     gene            complement(2542..>2871)
                     /gene="sucC"
                     /locus_tag="VGA15_06785"
     CDS             complement(2542..>2871)
                     /gene="sucC"
                     /locus_tag="VGA15_06785"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439181.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
                     /protein_id="HEX9587438.1"
                     /translation="GMEPANFLDVGGGADKAKVTAAFKIITADPNVKGILVNIFGGIM
                     KCDIIAEGVVAAVKEAGLKVPLVVRLEGTNVDAGKKIIRESGLNVLPADDLDDAAQKI
                     VKAVKGG"
BASE COUNT          527 a          890 c          865 g          589 t
ORIGIN      
        1 caagatgata tttgacgtcg ccggagccct cgaccgcgtc cggattgacc gagccgccct
       61 tgaattcatg aaacagcgcg cgatgcggct tgcccatcac ctgggtcagc acgttcaggc
      121 gtccgcgatg cggcatgccg aagacgattt ccttgacgcc gagattgccg ccgcgcttga
      181 tgatctgctc cagcgccgga atcagcgatt ccgcgccgtc cagaccgaag cgcttggtgc
      241 cggtgaattt gagatcgcag aatttctcaa aaccctcggc ctcgaccagc ttcgtcagga
      301 tcgcgcgccg gccctcgcgg gtgaaactga tttccttgtc cggaccctcg atgcgctcct
      361 gaatccaggc cttttgcgcg gcattgctaa tatgcatgaa ttcgacgccg agcgtctggc
      421 agtaggtgcg ttcgcagatc gtaaggatct cacgcaacgt ggcgtattcc aaacccagca
      481 catgatcgag gaagatcttg cggtcgaaat cgccctccgt gaagccgtag gaacgcggat
      541 cgagttcttc gcggtcgcgc ggcggctcga tcccgagcgg atcaagtctt gcgtggaaat
      601 ggccgcgcat ccggtaggcc ctgatcagca tcagcgcacg caccgaatcg cgcgtcgcct
      661 gatggatatc ggccgccgtc acttcggcgc ccttggtctg cgccttggcc gcgattttga
      721 tcccgacagt cttttcgact tccgcccagt taccatccag cgcggaagta agctcgtcgc
      781 gcggcgccag cggccagttg ttccgggccc aggacggtcc ttcggcattc ttgcggacgt
      841 cggcgggccg gtccttcagg ctcttgaaaa actcctgcca ctcggcgtcc accgaggcgg
      901 agtccctctc gtagcgggcg tagatatcgt cgatgtaggg agcatttgtg ccctgcaaaa
      961 acgaggagag ggcaaaagcg gcgttcgcgt cctggcgaga catgaatggc gtcctagcaa
     1021 tttcgactag cgcatggaaa acggcgcaac agccgatccg ggcaccggat cagcatgata
     1081 actggccttt taccttattt aggacaaatg ttctcgccga aaagaccaaa caatgaatta
     1141 atgcttcaac ttttcgacaa gggtcttgcc gagccgcgcg ggagacggcg agaccgcgat
     1201 tcccgccgct tccatcgccg cggtcttgga acccgcatcg cccttgccgc cggaaatgat
     1261 cgcgccggca tggcccatgc ggcgtccggg aggtgcggtg acgccggcga tgaatccaac
     1321 catcggcttc ttgcggccgc gcttggcttc atccttgatg aactgcgcag cgtcctcttc
     1381 ggccgaaccg ccaatttcgc cgatcatcac gatcgaggtt gtcttagggt cggccagaaa
     1441 catctcgagc atgtcgatga attcggtgcc cttgaccgga tcgccgccga tgccgaccgc
     1501 ggtggtctgg ccaaggccct cctgggtggt ctggaacacg gcctcataag tcagcgtgcc
     1561 cgatcgcgaa acgatgccga cattgccggg cttgaaaatg ttcgccggca tgatgccgat
     1621 cttgcattcg ccggcggtca tcacccccgg gcagttcggt ccgatcaggc gggatttcga
     1681 ccccgacagc gagcgtttca cccgcaccat gtcgagcact ggaatgcctt cggtgatgca
     1741 gacgatcagc ggaatctcgg cgtcgatggc ctcgcagatc gcgtccgcag cccccggcgg
     1801 tggcacatag atcacggatg cattggcgcc ggtcttttcc ttcgcctcgg ccacggtgtc
     1861 gaacaccggc agcccaagat gcaccgcgcc acctttgccc ggcgaggtgc caccgaccat
     1921 cttggtgcca taggcgatcg cggcctcgga gtggaacgtg ccgttcttgc cggtaaagcc
     1981 ctggcagatc acttttgtat ttttgtcgat caggatggac atggttgagg ctgctttcgc
     2041 gaacggtcgg taagggttca ggggatgcgc cggtagacgc cggtgagcac gtcggcccat
     2101 ccttcggcat ctggcgaaaa ctggatcttc atggtgtagg tgttgtcgtc gacgacttca
     2161 tagacatgac gcgcattgcc gcgcagcgag ccgcgcacca caatcagcgt cttgccggtc
     2221 cagccgccgg aggccggcgc cggggagtaa tatccgagcg aatcgtgcca gaacaatttg
     2281 tagtgccggt cttcgcggtc gtaggtgaac acgccatggg tggcgaaggt ttccttgccg
     2341 tcgcgcatct ggcgcgtgtc ctgaatcaga taaaagccgt tgagatccat gcgcgcgatg
     2401 acatgcgagg tggcggggcc gcccgcggtc cagcgcgatg gaaaaaccac ctcttcaccg
     2461 ttccactcgc ccgcaaaaac cgcgagcttg cggtgctcct ccagcggtga tggtgcggcg
     2521 agatggtcct cgggcattgg cttaccctcc cttgacagcc ttgacgatct tttgcgcggc
     2581 gtcgtcgaga tcgtcggcgg gcaatacgtt gaggccggat tcccggatga tcttcttgcc
     2641 ggcatcgaca ttggtgcctt ccagccgaac caccaacggc actttcaggc cggcttcctt
     2701 caccgcggcg accacgccct cggcgatgat gtcgcatttc atgataccgc cgaagatatt
     2761 gaccaaaatg ccctttacat tgggatcggc ggtgatgatc ttgaacgccg cggtgacctt
     2821 ggccttgtcg gcgccgccgc cgacatcgag gaagttcgcg ggctccatgc c
//
LOCUS       DASWGM010000223         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2514346,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000223 DASWGM010000000
VERSION     DASWGM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2514346"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VGA15_06790"
     CDS             complement(<1..950)
                     /locus_tag="VGA15_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010952952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX9587439.1"
                     /translation="MKRFVEGVDRGQSTLFPECLDDWIDEDNPVRVIDVFVDELDLGA
                     LDFGGVDPKATGRPSYHPSMLLKLYVYGYLNRVQSSRRLEREAGRNVEAMWLTGRLVP
                     DHKTIADFRKDNGRAIRQVCSRFVVLCRTMGLFAEAGVAIDGSKFKAVNNRDKNFTRA
                     KMQRRMAQIEESVARYLHQLDSADRQEPSLARTTKSTRLNEKIAKLKEEMQRLKALDV
                     QMLATPDQQISLTDPDARSMATSGRGSGVVGYNVQVAVDTKHHLIVTHEVTNVGNDRS
                     QLAGVAKQTKDTLRRESLDVVADRGYFNSTEILACEEAGIT"
     gene            complement(1075..1974)
                     /locus_tag="VGA15_06795"
     CDS             complement(1075..1974)
                     /locus_tag="VGA15_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9587440.1"
                     /translation="MDRLAAMEAFVRVVEAGSFSGAAKQLRVGQPAVSKTIAQLEDRL
                     GVRLLLRSTHGLTPTDAGRDFYEHAKRSIEDVEEAEAAARGAAVSLSGRLRVNAPVTF
                     ARLHVIPRLPIFMAEHPLLDVDVVLDDRDIDLIEAGIDVALRMGRLGDSGTTVRNIGH
                     CRSCVVGTPSYFAAHGRPKAPGDLIEHQAVIYEQRAGGPTWTFRQGTTETTVTVRGRL
                     RVSAAEGVREGVLAGLGLSIGGSEWLFTPELKSGAVISVLQDWSLPPMDLWALFPAGR
                     RASAKARAFTSFIERHLAGGEVV"
     gene            2129..2866
                     /locus_tag="VGA15_06800"
     CDS             2129..2866
                     /locus_tag="VGA15_06800"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9587441.1"
                     /translation="MTSKLSGKVALVTGGSAGIGLGIAKRFAEEGARVFITGRRQSEL
                     DKAVTAIGGGATAVQGDISNLDDLDRIYATIKDKAGRIDVLAVNAGYYEFGSFGEITE
                     EHFDKIFNTNVRGLLFTVQKALPLLTERASVILTGSIASIKGFPSLSIYNASKAAVRS
                     FARSWIADLKGRGIRINVMSPGTVETPGLSNLMAAEQKAEAVALVPLGRIGTPDDLGK
                     AAVFLASDDSAYITGIELFVDGGAAQI"
     gene            2925..3281
                     /locus_tag="VGA15_06805"
     CDS             2925..3281
                     /locus_tag="VGA15_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587442.1"
                     /translation="MSNDRGKIRFGLWYDFRNPAQWRQPADRLYREILDQIAWGENNG
                     FDDVWLSEHHFIEDGIFPRSCRPPPRSPPRTNRIHPKPFTAARMARIPRRRSAVDGQF
                     VADADDLHQHGFRQLC"
BASE COUNT          663 a         1081 c          997 g          622 t
ORIGIN      
        1 gtaatgccgg cttcctcgca cgccaggatc tccgtgctgt tgaagtagcc tcgatccgcg
       61 acgacatcga ggctctccct ccgcagcgtg tctttggtct gcttggccac gccggcgagt
      121 tgcgaccggt cgttgccgac attggtcacc tcatgcgtga cgatcagatg atgcttggtg
      181 tcgacggcga cctgcacgtt gtagcccaca acgcctgagc ctcggccgct tgtggccatc
      241 gaacgggcat cgggatcggt aagcgatatc tgctggtccg gcgtggccag catctgcacg
      301 tcgagtgcct tcagccgctg catttcctct ttcagctttg cgatcttctc gttgaggcga
      361 gtggactttg tggtgcgcgc cagcgaaggc tcctgccggt cggcgctatc gagttgatgc
      421 aaatagcgcg cgacgctctc ctcgatctgt gccattcgcc gctgcatctt ggcgcgcgtg
      481 aaattcttgt cccgattgtt gaccgccttg aacttgctgc catcgatggc gacgccagct
      541 tcagcgaaga ggcccatcgt gcggcacagc acaacaaacc ggctgcagac ctggcggatg
      601 gcccggccgt tgtccttgcg gaaatcggcg atcgtcttgt gatcgggaac aaggcgtccc
      661 gtcagccaca tggcctccac attgcgtccg gcctcgcgct cgagccgtcg gctcgactgg
      721 acccggttca gataaccgta aacatagagc ttcaagagca tcgacggatg atacgagggt
      781 cgccctgttg ccttcggatc gaccccgccg aaatcgagcg caccgagatc aagctcgtcg
      841 acaaacacgt caatcactcg aaccgggtta tcctcgtcga tccaatcatc caggcattca
      901 ggaaacaacg tcgactgccc tcgatccacc ccctcgacga agcgcttcat cgcgacctcc
      961 ccccgaaaat cgcgtcacaa gcttatcatg cggggcgttt tcacacagcc tggaccctaa
     1021 gccgtcattt cgagcgaggc gcgctccggg acgatacccg cgcttaattc acctttagac
     1081 aacctctccg cccgcgaggt ggcgctcgat gaagcttgtg aacgcgcggg ctttggcgct
     1141 ggcccgtcgt ccggccggaa agagcgccca tagatccatc ggcggcagcg accaatcctg
     1201 cagcacggaa atgaccgccc cggatttcag ctccggggtg aacaaccatt ccgagccgcc
     1261 aatcgaaagg cccaaaccgg cgagcacccc ctcacgaacc ccctccgccg cgctgactcg
     1321 aagtcgaccg cgcaccgtga cggtcgtttc tgtcgtccct tggcgaaagg tccaggtagg
     1381 accgccggca cgctgctcgt agatgacagc ttgatgctcg atgaggtcgc cgggggcctt
     1441 cggtcgaccg tgcgctgcga aataggaggg cgtaccgacc acacagctgc gacagtgtcc
     1501 gatattgcgg acggtcgtgc ctgaatcgcc gagccggccc atgcgcagcg cgacatctat
     1561 cccggcctcg atcagatcga tatcccgatc atcgagcaca acgtcgacgt cgagcaatgg
     1621 gtgctccgcc ataaaaatcg gcagccgcgg tatgacgtgg agacgggcga aggtcaccgg
     1681 tgcattgacc cgcagtcgcc ccgacaacga tacggcggcg ccgcgagccg cggcttccgc
     1741 ctcctcgaca tcctcgatcg accgtttagc gtgctcatag aagtcgcggc ctgcatcggt
     1801 cggcgtcaac ccgtgcgtcg atcgcagcaa cagccgcacg ccgagccggt cttcaagctg
     1861 tgcgatggtt ttggagacag cgggttgccc gacgcgaagc tgcttggccg cgccggaaaa
     1921 cgagcctgcc tcgaccaccc gaacaaaggc ttccattgcc gccaatcggt ccatctcggc
     1981 agcccgcgga cagtcattcc tgacaggaat aatcgataat ccacggtggc ggctactgcg
     2041 acatcggtca aaacgcaagt tcaccatagc ggcgatgcac gttgctggac gccgccatgg
     2101 gtatcgcgca atcgcaggag atctcacgat gaccagcaag ctatcaggca aagtcgcttt
     2161 ggtgaccggc ggcagtgccg gcatcggcct cggcatcgcc aaacgttttg ccgaggaagg
     2221 cgcgcgcgtg tttatcaccg ggcgtcgcca atccgagctg gacaaggccg ttaccgcgat
     2281 cggcggcggc gccaccgccg ttcaaggcga tatctctaac ctcgacgacc tcgaccgcat
     2341 ttacgcgacg atcaaggaca aggcgggacg tatcgacgtg ctggcggtca atgcggggta
     2401 ttacgaattc ggcagcttcg gcgaaatcac cgaggagcat ttcgacaaga ttttcaacac
     2461 caatgtgcgc ggcctgctgt tcacggtcca gaaggcgctg ccgctgttga ccgagagggc
     2521 atcggtgatc ctcaccgggt cgattgcctc gataaaaggc ttcccgtcct tatcgatata
     2581 caacgcaagc aaggccgcgg tgcgctcctt cgcgcgtagc tggatcgccg acctgaaggg
     2641 gcgtggcatc cgaattaatg taatgagccc cggcaccgtc gagacacccg gcctctccaa
     2701 tctgatggcc gcggagcaga aggcggaggc agtcgctctg gtcccgttag gccggatcgg
     2761 gacacccgac gatctcggta aggccgcagt gttcctcgct tccgacgaca gtgcctacat
     2821 caccggcatc gagctgtttg tggacggtgg cgcggcgcaa atctgagccg ggcttctgag
     2881 gggaccaata ccaggcggcc cctgcctgcc aggagagagg catcatgagc aatgataggg
     2941 gaaaaatccg cttcggcctt tggtacgact tccgcaaccc agcacagtgg cgccagccag
     3001 ccgatcggct ctaccgcgaa atcctcgatc agatcgcgtg gggcgaaaac aacggtttcg
     3061 atgatgtctg gctgtccgag caccacttca tcgaagacgg tatcttccct cgatcctgcc
     3121 ggccgccgcc gcgatcgccg ccccgtacca accggatcca tccaaagccg ttcaccgccg
     3181 cgcgtatggc gcgtatcccg cgccgaagat cagcggtcga tggccagttc gtcgccgatg
     3241 cggacgatct acaccagcac ggttttcggc aactctgctg acaggtggtc gatgaaggcg
     3301 cgaactgaag ggagaaggcc gcgccgatag ggtatgaggg cagtgatcgt ggaatcaccg
     3361 gca
//
LOCUS       DASWGM010000224         5366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2238558,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000224 DASWGM010000000
VERSION     DASWGM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238558"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..647)
                     /locus_tag="VGA15_06810"
     CDS             complement(138..647)
                     /locus_tag="VGA15_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9587443.1"
                     /translation="MSTTLIEVRPAKAADAAAVASTHDEAWRAAYQGIIPGAELEKLI
                     NRRGPQWWDSAIRKGSRVSVLVFGDKVAGYANYGRNRARSLHFEGEIYELYLRPEFQG
                     LGFGRRLFTSARRDLLQSGLKSMVVWALSDNDPATEFYRALGGRMVARSSEKFGPKSL
                     DKVAFAWTN"
     gene            926..1825
                     /locus_tag="VGA15_06815"
     CDS             926..1825
                     /locus_tag="VGA15_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9587444.1"
                     /translation="MQSCKAFFVLVVLGLVGAQSAIAQPRFGAQGAEAEPARMQQWLV
                     PSPDPQTAAHALLFRPSGDGPFPLALITHASMQNVLRRAQMPQPEYRALAAWLVARGF
                     AVLVPERPGHGATGGKYLEDQGGCDEADYSRSGYATADSIKAALGYLREQPFVRQDGT
                     VIVGHSAGAWGALALAGENPKGLSAIIAFAPGRGGHANDLPNQVCAPHTLIQSAAEFG
                     EDTHVPVIWLVATNDSYFSPDLSRQLADAFRTGGGRVDFRVLPASGSEGHWLAETEAG
                     VKIAGPELDSALKALAPAAAKKR"
     gene            1837..2244
                     /locus_tag="VGA15_06820"
     CDS             1837..2244
                     /locus_tag="VGA15_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX9587445.1"
                     /translation="MKPWPDDDVILYDGVCVFCSRWIRFVAARDKARRFRFTAIQSGY
                     GTRLAQAFGINPDDPDTNAVVHGGVAHFKSDGALTVLSNLPGWGWVRVLLSVPKPLRD
                     PVYNLVARNRYRIFGRYDACFVPDAELKARVME"
     gene            2340..3242
                     /locus_tag="VGA15_06825"
     CDS             2340..3242
                     /locus_tag="VGA15_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9587446.1"
                     /translation="MTKAVFTTKVTPSYKDLPEERYHFPRTYLNYVQQTVGDYIVYYE
                     PRRSSAELSSRGGRQSYFGVARVDSVVEDPALPGHYFAIIDRATYLDFDAAVPFYEGD
                     EYYESALKKDDGSTNKGAFGRAVRLIPDGEFDRILKSGFAPILGENLTSEVTPPGFSE
                     PPAPFERPIVEMMVSRPFRERSFMHNVRAAYSNRCAITGLRLINGGGRPEVQAAHIQP
                     VASKGPDSIRNGLALSGTVHWMFDRGLISIGDDYKILVAENHVPEDAVRLLNKNGVIH
                     LPKDQTLYPNAHYLKFHRDQVFKG"
     gene            complement(3281..4525)
                     /locus_tag="VGA15_06830"
     CDS             complement(3281..4525)
                     /locus_tag="VGA15_06830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9587447.1"
                     /translation="MSLPSEVDVAIIGAGAAGLGAARALENSGLSVVVLEARSRVGGR
                     AHTIMAAPDITFDLGCGWLHSGDINSFVQIAGQLDFEIDKTRPPWREQSFDIGFPPRE
                     RADFIEALDAFYDRAEEAAKSGRDNAASEYLEPGNGWNPMIDAISTYVNGCELDRVSI
                     LDMEAYEDTEINWRVRRGYGALMEAYGAPCPLALNCEVKRIDHSSKRVRIETSQGRLS
                     ADKVIVTIPTNLIACEALRFHPELPAKIDAARGLPLGLADKVMLALDEPEALPKDGNL
                     RGATMRTAMGGYHLRPFGQPCIEGFFGGRFARELEDAGDGALAAQSIDEIAALLGNDF
                     RRKLKPLSESRWAHDPFAQGSYSHALPGHAGKRAVLAAPVDGRLFFAGEATSPNFFST
                     AHGARDSGERAAREVLASVAKR"
     gene            complement(4615..>5366)
                     /locus_tag="VGA15_06835"
     CDS             complement(4615..>5366)
                     /locus_tag="VGA15_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX9587448.1"
                     /translation="RGELQLAILIETMRREGFELSVSRPRVVLKKDEATGEWQEPVEE
                     VVNDVDEEHSGVVVQKMSERKAELIEMRPSGGHRLRLVFYAPTRGLIGYQGELLTDTR
                     GTAIMNRLFHGYAPYKGDIQGRRNGVLISNDQGEAVAYAMFKLEDRGPMMITPGAKVY
                     KGMIVGEHTRDNDLEINILKGKQLTNIRTTSKDEAVRLTPPIQMTLEKALAYIEDDEL
                     VEVTPKSIRLRKKFLDANDRKRAEKAKEAVA"
BASE COUNT          979 a         1646 c         1665 g         1076 t
ORIGIN      
        1 acctgacgcg gcggatcgat cccgatcgag atggcctcaa tgcgcatgga tgctccgcat
       61 ggttttggga actggcttta gatcacgggc ggcagctcgg cccgagccgc agcagcacgc
      121 cttgaaggcc ggcgaactca attggtccag gcgaacgcga ccttgtcgag cgacttgggc
      181 ccgaatttct ccgacgaacg cgctaccatg cggccgccaa gggcgcggta aaactccgtc
      241 gcgggatcgt tgtcggagag cgcccagacc accatgctct tcaggccgct ctgcagcagg
      301 tcgcgccggg cggaggtgaa cagccggcgg ccaaagccaa gaccctgaaa ttccggccgc
      361 agatacagct cataaatctc gccttcgaag tgcaggctgc gcgcacggtt gcggccgtaa
      421 ttggcgtagc ctgcgacctt gtcgccgaac accagcacgc tgacgcggct gcccttgcgg
      481 atcgcgctgt cccaccattg cgggccgcgg cggttgatca gcttctccag ctcggcacca
      541 ggaatgatgc cctgataggc cgcgcgccag gcttcgtcat gggtggacgc caccgcggct
      601 gcatccgcag ctttggccgg ccgaacctcg atcagggttg tgctcatggt tctaatcaaa
      661 gcaattcagc gcgtccgcat caaggtccat cgttaattat cggttaacct gtggattttg
      721 ggcatcagtt ttaccgtctg gttgtaccga aagaggacag ggacgtgttt taggcgacat
      781 ttcgatcggg gttggcggac tacgacgagc ggaccaaacg ttttggcagg ccgcgttcac
      841 ggaaaaatgc actggatttg attcgccatg ggtattctgc cgcgcgaatc cgagtccgtt
      901 tttccggttg gcgtcagggc aattcatgca atcgtgcaag gcgtttttcg ttctggttgt
      961 gcttggactc gtcggtgcgc agagcgcgat agcgcagccg cggttcggtg cgcaaggcgc
     1021 tgaagctgag cccgcccgca tgcagcaatg gctggtgccg tcgcctgatc cccaaaccgc
     1081 ggcgcacgca ctgctgttcc gcccctcggg cgatgggccg tttccgctgg cgctgattac
     1141 ccacgcctcg atgcaaaacg tgctgcggcg cgcccaaatg ccgcagcccg aataccgcgc
     1201 gctggcggca tggctggtcg cgcgcggctt tgccgtgctg gtgccggaac gtccggggca
     1261 cggcgcgacc ggcggaaaat atctcgagga ccagggcggc tgcgacgaag ccgactattc
     1321 ccgttccggc tatgcgacgg cggactcaat taaagccgcg ctcggctatc ttcgcgaaca
     1381 gccgttcgtc cggcaggacg gcacggtcat tgtcgggcac tcggctggcg cctggggcgc
     1441 gctggcgctg gccggtgaga atccaaaagg cctctccgcc atcatcgcct tcgcgccggg
     1501 gcgcggcggt cacgccaacg accttcccaa tcaggtctgc gcgccgcaca cgctgattca
     1561 atccgcggcc gaattcggcg aagatacgca cgtgccggtg atatggctgg tggcgaccaa
     1621 tgacagctat ttctcacccg atctgtcgcg gcagctggcc gatgcattcc gcaccggcgg
     1681 cggaagagtg gactttcgcg tgctgccggc atccggcagc gagggccact ggctggcgga
     1741 gaccgaggcc ggcgtcaaga ttgccggacc ggaactggac agcgcgctga aggcgcttgc
     1801 gccggccgcg gcgaaaaaac gatgacggtt ggcacgatga agccatggcc ggacgacgac
     1861 gtgatcctct acgacggcgt ctgcgtgttt tgctcgcgct ggatccgctt tgtcgccgcg
     1921 cgcgacaagg cgcggcgttt tcgcttcacg gcgatccagt ccggctacgg cacgcggctg
     1981 gcgcaggcgt tcggcatcaa ccccgacgat cccgacacca acgcggtcgt tcatggcggt
     2041 gtcgcccatt tcaaatccga cggggcgcta acggtgctgt ccaatttgcc gggctggggc
     2101 tgggtgcgcg tgctgctctc agtgccgaag ccgctccgcg atccggtcta caatctcgtc
     2161 gcccgcaacc gctaccggat ttttggaagg tatgacgcgt gctttgttcc ggatgctgag
     2221 ctgaaggcga gagtgatgga ataagggaag gcgagggaat cgctgctgac tatctaagcg
     2281 atcaaagctg cactaagctg aagttggccg agatcgcgag ctgtcgcagg ctttagttta
     2341 tgacaaaagc agttttcacc acgaaggtta ctccgagcta caaagacctg cctgaggagc
     2401 gataccattt tccgcgtaca tatctaaact atgtccaaca gaccgtgggc gattatatcg
     2461 tctactatga gccgcgcaga tccagcgcag aactttcaag tcgcggaggc cgccaatctt
     2521 actttggcgt ggcccgagtt gactcggtgg ttgaagatcc ggcgctgcca ggtcactatt
     2581 tcgcaataat tgatcgcgcc acctatctgg atttcgacgc cgccgtccct ttctatgagg
     2641 gcgatgagta ctacgagagc gcgctcaaga aagatgatgg cagcaccaac aagggtgcct
     2701 ttggccgcgc cgtgcggctg attccggatg gtgagttcga tcggatattg aagtctggtt
     2761 ttgctccaat tctgggcgag aatctaacgt cagaagttac gccgccagga ttctcggaac
     2821 ctcccgcgcc gttcgagcgg ccgatcgtcg aaatgatggt ctctcggcca ttccgcgagc
     2881 gatccttcat gcacaatgtg cgcgctgcct actcaaatcg gtgcgccatc acgggcctgc
     2941 ggctgatcaa tggaggaggc cgtcccgaag ttcaggcagc gcacattcaa cctgttgcgt
     3001 caaaaggtcc ggactccatt cgcaacggac tggctctgtc cggaaccgtg cactggatgt
     3061 ttgatcgggg actcatttcg attggcgacg actacaagat ccttgtcgct gagaaccacg
     3121 ttccagaaga tgccgttcgt cttttgaata agaacggtgt gattcactta cccaaagacc
     3181 aaactcttta cccgaacgcg cattacctga aatttcatcg agatcaagtc ttcaaaggtt
     3241 agagccgaac agtcgccatt cgcaataacg agatcgacac tcatcgcttc gcgaccgaag
     3301 ccagcacttc cctcgccgcc ctctccccac tgtcccgcgc gccgtgcgcg gttgaaaaga
     3361 agttcggcga ggtggcctcg ccggcaaaga acaaccgccc gtcaaccggt gctgcgagca
     3421 ccgcgcgctt ccccgcgtgg ccgggcaggg cgtgcgagta ggagccttgg gcgaacgggt
     3481 cgtgcgccca gcgcgattcc gacagcggtt ttagtttgcg gcggaaatca ttgccgagca
     3541 gtgctgcgat ctcgtcgatg ctttgtgcgg cgagtgcgcc gtcgccggca tcttccaatt
     3601 cccgcgcaaa gcggccgccg aaaaaacctt cgatgcaggg ctggccgaac gggcgcagat
     3661 gataaccgcc cattgcggtg cgcatggtgg cgccgcgcag attgccgtcc ttcggcagcg
     3721 cctccggttc gtcgagcgcc agcatgacct tgtcggcaag gccgagcggc aggccgcgcg
     3781 cggcgtcaat ttttgctggc agctcagggt gaaatcgaag cgcctcacag gcgatcaggt
     3841 tggtgggtat ggtgacgatc accttatcgg ccgaaagccg gccttgcgat gtctcgatgc
     3901 gcacccgctt gctggaatga tcgatgcgct tcacctcgca attgagcgcg agcgggcaag
     3961 gcgcgccata ggcctccatc agcgcaccat agccgcgccg tacccgccaa ttgatctcgg
     4021 tgtcctcgta ggcctccatg tcgaggatcg agacgcggtc gagttcgcag ccgttgacgt
     4081 aggtcgagat cgcatcgatc atcgggttcc agccattgcc gggctcgaga tattcgctcg
     4141 cggcattgtc gcggccgctt ttcgcggcct cctcggcgcg gtcatagaac gcatcgagcg
     4201 cttcgatgaa gtcggcgcgc tcccttggcg ggaacccgat gtcaaaactc tgctcgcgcc
     4261 agggtggacg ggttttgtcg atttcgaaat cgagttgccc ggcgatttgg acgaacgaat
     4321 ttatgtcgcc cgaatgcagc cagccgcagc cgagatcgaa ggtgatatcg ggggcggcca
     4381 tgatggtatg ggcacgcccg ccgacgcggc tccgcgcttc cagcacgacc accgagaggc
     4441 cggagttttc cagcgcccgc gcagccccaa ggccggcggc gcccgcaccg atgatggcga
     4501 catcgacttc ggaggggagg gacatgaaag ggcgaatggt gagtggcgaa ttgcgaatag
     4561 aacgtcactt atatggttcg tctactcgcc attcgccact ccctattcgc ccacttaagc
     4621 caccgcttcc ttggcctttt ccgcgcgctt gcggtcgttg gcgtcgagga actttttgcg
     4681 cagccggatc gatttcgggg tcacctcgac cagttcgtca tcctcgatat aggccagcgc
     4741 cttttccagg gtcatctgga tcggcggcgt caggcgcacc gcttcgtcct tcgacgtggt
     4801 acggatattg gtgagctgct tgcccttcag gatgttgatc tcgaggtcgt tgtcgcgggt
     4861 atgttcgccg acgatcatgc ccttgtagac cttggcgccg ggcgtgatca tcatcgggcc
     4921 gcggtcctcc agcttgaaca tcgcataggc cacggcctca ccctgatcgt tggagatcag
     4981 gacgccgttg cggcggccct gaatgtcgcc cttgtagggc gcgtagccgt ggaacagacg
     5041 gttcatgatc gcggtgccgc gggtgtcggt cagcaattcg ccctgatagc cgatcaggcc
     5101 tcgggtcggc gcataaaaca ccagccgcag ccggtggccg ccggaggggc gcatctcgat
     5161 caactcggcc ttgcgttcgc tcatcttctg cacgaccacg ccggaatgtt cctcgtcgac
     5221 gtcgttcacg acctcttcga ccggctcctg ccactcgccg gtcgcctcgt ccttcttcag
     5281 gacgacgcgc gggcgcgaca ccgacagctc aaagccttcg cggcgcatgg tctcgatcag
     5341 gatcgcgagc tgcaattcgc cgcggc
//
LOCUS       DASWGM010000225         5565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_293420,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000225 DASWGM010000000
VERSION     DASWGM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293420"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..736)
                     /locus_tag="VGA15_06840"
     CDS             complement(251..736)
                     /locus_tag="VGA15_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX9587449.1"
                     /translation="MIETSQTRPRACSPRGLAARWRGMRALAAIALVSAVLGGCTGEQ
                     FQKGYILPPGALEQIPIGASQDQVLIVMGTPSTVATLNGEVFYYISQRSERKVAFMNQ
                     QVVDQRVIAIYFDKSRQVQRLANYGLQDGKIFDFISRTTPTSGQELSYLTPLFKLLSF
                     N"
     gene            844..1395
                     /locus_tag="VGA15_06845"
     CDS             844..1395
                     /locus_tag="VGA15_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEX9587450.1"
                     /translation="MLWPFNHFRKPRISSRGTIEAIYGMIVTQAREPLFYRDLGVPDT
                     VNGRFDLVVLHLWMVLHRLKSMEGAADLSQALFDHFCDDMDANLREMGVGDLTVPKRM
                     RAFGEAFYGRAAAYDLALTAGGEPLAQALCKNILNGGEIEKARQLAFYTEAAIAVLCG
                     LDDATLQKGSWRFPSPARVGVQQ"
     gene            1410..1982
                     /locus_tag="VGA15_06850"
     CDS             1410..1982
                     /locus_tag="VGA15_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX9587451.1"
                     /translation="MTNKADPWSVPVTVTQIPDTGLHRDIEADAATLEAMAEIAGLRE
                     ILFARASLDVTPKGGGRVQVAGRVWARVGQSCVVTLDPVENDIDEPIDLIFAPPEQIP
                     QLADLVDDAAESDAEIPDPPEPIENGVINLGRLATDALFLGIDPYPRRPDAVFEPPAA
                     IADPEDHPFAALKALQLDAKPTGAKKPRNR"
     gene            2293..3354
                     /gene="plsX"
                     /locus_tag="VGA15_06855"
     CDS             2293..3354
                     /gene="plsX"
                     /locus_tag="VGA15_06855"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX9587452.1"
                     /translation="MPQKVRIALDAMGGDVGASVVIPGAAISLTRHPDTEFLLFGQSG
                     LIEAELAKHPAMKAASRVIHTDVAVSNNDKPSQALRRGRKTSSMWLAIDAVKKGEADV
                     AVSAGNTGALMAMSRFCLRMLPGIDRPAIAAVWPTMRGDSVVLDLGATIGGDAHHLMA
                     LAVMGSAMASVLFDLERPTVGLLNIGVEEVKGREEIREAAELLRAMNLPQLEYIGFVE
                     GDGIGKGAADVIVAEGFSGNIALKATEGTARQINDFLRAALSRTWRSRIGYLFAKNAF
                     KALRDKLDPNKSNGGVFLGLNGIVVKSHGGTDAEGFAYAVDVGYEMVRYDLLTKINQM
                     LNRDGSALPPAPTAPEAVS"
     gene            3351..4328
                     /locus_tag="VGA15_06860"
     CDS             3351..4328
                     /locus_tag="VGA15_06860"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX9587453.1"
                     /translation="MTAIRSVVLGCGSYLPQQILTNAELAARIDTSDEWIVQRTGIRQ
                     RHIAAEGEFTSHLAIHAARAAIANAGIDAQSIDLIVLATSTPDNTFPATAVAVQNGLG
                     INHGAAFDLQAVCSGFVFALATADNFLRTGAFKRALVIGAETFSRILDWNDRGTCVLF
                     GDGAGAIVLEAESQPGTRSDRGILTSHLRSDGRHKSKLYVDGGPSSTRTVGYLRMEGR
                     EVFKHAVGMITDVIVDAFEATGTTAEDIDWFVPHQANKRIIDASAHKLHIAPQKVVLT
                     VDLHGNTSAASIPLALAVAVADGRVKKGDLVLLEAMGGGFTWGSALVRW"
     gene            4459..4788
                     /locus_tag="VGA15_06865"
     CDS             4459..4788
                     /locus_tag="VGA15_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX9587454.1"
                     /translation="MTGTGKTVTRVDLCEAVYQKVGLSRTESSAFVELVLKEITDCLE
                     KGETVKLSSFGSFMVRKKGQRIGRNPKTGTEVPISPRRVMVFKPSAILKQRINGNGTV
                     NGDVKAD"
     gene            4830..5408
                     /locus_tag="VGA15_06870"
     CDS             4830..5408
                     /locus_tag="VGA15_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX9587455.1"
                     /translation="MEKSPDAFRTISEVAADLDIPQHVLRFWETRFAQIKPMKRSGGR
                     RYYRPDDVDLLRGIRRLLYGEGYTIRGVQRILKEHGIASVQRLADGSAIASFGAIEEA
                     VGLSILEDGPAPAPGIDVDDDDYEGATDGIDFRLLNDDDDDEEELSAAFGGGPIPGVD
                     SLTRPLDVARLEAVLQDLIACRQLLDSALKEG"
BASE COUNT         1038 a         1659 c         1779 g         1089 t
ORIGIN      
        1 atccctacaa ggatacggcg ggccccgccg tcaatccgat gatcaagatc accaacatcg
       61 tggcgctctt gctgctggcg gtcctggcgc actgagcgcg cgagatatga tcgacaaaaa
      121 ccccgcggtt cgcgccgcgg ggtcttttcc cttttattgc gatcgctcaa ccgtcgtttc
      181 ccgcatgctg cgcagcgcgc cgctcttggg gcgtggtgca tcgcgtccgg gacacgatgc
      241 acgacgtgcc ttagttgaag ctgagcagct tgaacagcgg cgtcaggtag ctgagttcct
      301 ggcctgacgt gggcgtggtg cggctgatga agtcgaagat cttgccgtcc tgcagcccgt
      361 aattggcgag ccgctgcacc tggcggctct tgtcgaaata gatcgcgatc acccgctgat
      421 cgacgacctg ctgattcatg aacgctacct tgcgctcgga gcgctgtgaa atgtaataga
      481 atacttcgcc gttgagggtt gcgaccgtgg agggcgttcc catcacgatc agcacctgat
      541 cctggctagc gccgatcgga atttgctcga gcgcgccggg cggcaggatg tagcccttct
      601 gaaactgctc gccggtacag ccgcccagca ccgcgcttac caaggcgatg gcggccaagg
      661 cgcgcatgcc gcgccagcgc gcggcgaggc cccgcggaga gcacgcgcgt gggcgtgtct
      721 ggctcgtttc aatcattccg gagctgatcc cgtcctcttg catcgcgcga ggcgttgacg
      781 taccgggcag caagcaggat tgcaacatgc gcgggccggg ttggctcgct tgcggaaccc
      841 acaatgcttt ggccgttcaa tcacttcagg aaaccccgga tatcatcgcg cggcaccatt
      901 gaggccatct atggcatgat cgtgacgcag gcgcgagaac cgttgtttta tcgggacttg
      961 ggcgtgccgg atacggttaa tggccgtttt gatctcgtgg tgctgcattt gtggatggtg
     1021 ttgcaccggc tgaagtcgat ggagggggct gcggaccttt cccaggcgct gttcgatcac
     1081 ttctgtgacg atatggacgc caatttgcgc gaaatgggcg ttggcgatct taccgtcccg
     1141 aagcgcatgc gggcgtttgg ggaggccttt tatggccggg cggcggccta tgaccttgct
     1201 ttgaccgccg gcggagagcc gctggcgcaa gcgctgtgca agaatatcct gaacggcggg
     1261 gaaatcgaaa aagctcggca gcttgccttc tatacggaag ccgccatcgc agtgctctgc
     1321 ggattggacg atgcgacgtt gcagaaggga tcgtggaggt ttccttcgcc ggcgcgggtg
     1381 ggcgtacagc aatgaacagg gcgaatcaca tgacgaacaa ggccgatccc tggagcgttc
     1441 cggtcaccgt gacgcaaata ccggataccg gactgcaccg cgacatcgaa gccgatgctg
     1501 cgacgctgga ggcgatggct gaaatcgccg gcttgcgcga aattcttttc gcacgtgcgt
     1561 cgctcgatgt gacgcccaaa ggcgggggcc gcgttcaggt cgcggggcga gtttgggcac
     1621 gggtcgggca gagctgtgtc gtgaccctcg atccggtcga aaacgacatc gacgagccga
     1681 tcgacctgat cttcgcgccg cctgagcaaa ttccgcaact cgctgatctg gtcgatgatg
     1741 ccgctgaaag cgatgcagag attccggacc cgcccgagcc gatcgaaaat ggcgtgatca
     1801 atctcggccg gctcgcaacc gacgcgttgt tcttagggat tgatccctat ccgcgcaggc
     1861 cggacgcggt tttcgagccg ccggccgcga ttgcggatcc cgaggatcac ccatttgccg
     1921 cgctcaaggc gctgcagctt gatgcaaaac cgaccggcgc gaagaagccc aggaaccgct
     1981 gaaacagcgc tgacgaaccg ctgaagcggc actgagctaa tcttgcaaca aacgaggttg
     2041 cgggaccggt accaaaaacc tctctagtgc tttgaattat ctagatatat ttattcgggg
     2101 cgcttccgcg ttgcagcggc tcggcctgta aaagagggcg gtcaagaggt tgtgtcaggg
     2161 cggggacacg gtattgtcgc ggcccggccg gggcgcagcg ttgcgcttcg ccgatcgccg
     2221 ctatccgtgg cgatttccag tccgcggcca aggcgcttca agtccgcaag agatcaggtt
     2281 tccgggacgt tcatgccgca aaaggttcga atcgcgcttg acgccatggg tggcgatgtc
     2341 ggcgcatcgg tcgtcattcc cggcgccgcc atttcgctga cccgacatcc cgacaccgaa
     2401 tttctgctgt tcggccagag tgggttgatc gaggcggaac tcgcaaaaca tccggcgatg
     2461 aaggcggctt cgcgcgtcat ccataccgat gtcgccgtca gcaacaacga caaacccagt
     2521 caggcgctgc gccgtggccg caagacctcg tcgatgtggc ttgccatcga cgccgtcaag
     2581 aaaggcgaag ccgatgtcgc ggtatccgcc ggcaacaccg gcgcgctgat ggcgatgtcg
     2641 cggttctgtc tgcgcatgct gcccgggatc gaccgtccgg cgatcgcggc ggtgtggccg
     2701 accatgcgcg gcgattcggt ggtgctcgac cttggcgcca ccatcggcgg cgatgcgcat
     2761 cacttgatgg cgctggcggt gatgggtagc gccatggcga gcgtgctgtt cgatctcgag
     2821 cggcccaccg tcggcctgct caatatcggg gtcgaggagg tcaagggccg cgaggaaatt
     2881 cgcgaggcag ccgaattgct gcgcgcgatg aatcttccgc aactcgaata tatcggcttt
     2941 gtcgaaggcg acggcatcgg caagggtgct gccgacgtca ttgtcgccga agggttcagc
     3001 ggcaatatcg ccctgaaagc gaccgagggc acggcgcgcc agatcaacga ttttctccgt
     3061 gcggcgctgt cgcgcacctg gcgttcccgg atcggctatc tgttcgcaaa aaatgccttc
     3121 aaggcgttgc gcgacaagct cgatccgaac aagtcgaatg gcggcgtgtt cctcggattg
     3181 aacggcattg tggtcaagag ccacggcgga accgacgccg agggctttgc gtatgcggtc
     3241 gatgttggct atgagatggt ccgctacgat ctcctgacca agatcaatca gatgctcaat
     3301 cgcgacggca gcgcgctccc tccagcgccg accgcgccgg aggctgtctc gtgaccgcaa
     3361 tacgttcggt tgtactcggc tgcggttcct atctgccaca gcagattctg accaatgccg
     3421 aactggcggc gcggatagac acttccgacg agtggatcgt gcaacgcacc ggtatccggc
     3481 agcgccacat tgccgccgag ggcgagttca cctcgcatct ggcgatccat gcggcacgcg
     3541 ccgcgattgc caatgccggg atcgatgcgc agtcgatcga cctgatcgtg ctcgcgacct
     3601 cgacgccgga caataccttt cccgccaccg cggtcgccgt ccagaacggg ctcggcatca
     3661 accacggcgc tgcattcgat ctgcaggcgg tatgctcggg ctttgtcttc gcgcttgcca
     3721 ccgccgacaa tttcctgcgc accggcgcgt tcaagcgggc gctggtgatc ggcgccgaga
     3781 cgttctcccg catcctcgac tggaacgacc ggggaacctg tgtgctgttt ggcgacggcg
     3841 ccggcgccat cgtgctcgaa gccgagtcgc agccgggaac ccgctccgac cgcggcatcc
     3901 tgacttctca cctgcgctcc gatggccggc acaagtcgaa attatatgtc gatggcggtc
     3961 cttcctcgac ccgcaccgtg ggttatctcc ggatggaggg ccgcgaagtg ttcaaacatg
     4021 ccgtcggcat gattaccgat gtgatcgtgg atgctttcga ggccaccggc accaccgccg
     4081 aggacatcga ctggtttgtt ccgcaccagg ccaacaagcg aatcatcgat gcttcggcgc
     4141 acaagctgca tattgcaccg caaaaagtgg tgctgacggt cgatcttcac ggcaacacct
     4201 cggcggcgtc gatcccgctg gcgctggcgg ttgcggtcgc cgacggtcgt gtgaagaaag
     4261 gcgacctggt gctgctggaa gccatgggcg gcggcttcac ctggggctcg gcactggtgc
     4321 gctggtaagc gccgcgctaa ggtgttatcc gcatttagac gttgttccat gcgactgcat
     4381 tgatgagcgc tgttgaccgt cgcttgttaa cctcataatt tcagatacat attgttcgcc
     4441 gtgagtttgg ggcaggcgat gaccggaacc ggaaagacag ttacacgcgt cgatctgtgc
     4501 gaagccgtct accaaaaggt aggcctctcg cgcacggaat cgtctgcttt tgtcgaactg
     4561 gtcttgaaag agatcaccga ctgcctggaa aagggcgaga cggtaaaatt gtcgtcgttc
     4621 ggatcgttca tggtccgcaa gaaaggccag cgtatcggcc gtaacccgaa aaccggtact
     4681 gaagttccca tttctccgcg tcgcgtgatg gtgttcaagc cgtccgccat attgaagcag
     4741 cgaatcaacg gcaacggtac cgtcaatggc gacgtcaaag ccgattagac aattcattta
     4801 aactcggcgc agccgagagg agcgggcatt tggagaagtc cccggatgcg tttcgaacca
     4861 tcagcgaagt cgccgccgac ctcgatatcc ctcagcacgt cctgcggttt tgggagacgc
     4921 ggttcgctca aatcaagcca atgaagcgca gcggcggccg gcgctattat cgcccggacg
     4981 acgtcgatct gcttcggggc atccgccgcc tgctgtatgg cgagggctac accatccgcg
     5041 gggtgcagcg catcctgaag gagcacggca tcgcctcggt gcagcggctg gcggatggat
     5101 cggcgatcgc ctcgttcggc gcgatcgaag aggccgtcgg cctgagcatt ctggaagacg
     5161 gtcccgcacc tgcgcctggc atcgacgtcg atgacgacga ttatgagggg gcgacggacg
     5221 gcatcgattt tcgccttctc aacgacgacg acgatgatga ggaagaactc tccgcggcgt
     5281 tcggcggcgg cccgatccct ggcgtcgaca gcctgacgcg cccgctcgac gtcgcgcgtc
     5341 tggaagcggt cttgcaggac ttgatcgcct gccggcaatt gctcgattcc gcgctcaagg
     5401 aaggctaggc ggcgttcagc ggacacaggc ttttccggcc gcccggtggg cccgatcgcg
     5461 ctctatcagg cttcttgcgc gcaagcctga tcgaagctaa tggaacggca tcggagcgtg
     5521 gcgcagcccg gttagcgcac tagtctggga gactaggggt cggag
//
LOCUS       DASWGM010000226         6926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_21265,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000226 DASWGM010000000
VERSION     DASWGM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21265"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="VGA15_06875"
     CDS             <1..109
                     /locus_tag="VGA15_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587456.1"
                     /translation="LLKNGTLKTYKDFPHGMPTTEAATINADLLAFIKG"
     gene            complement(138..752)
                     /locus_tag="VGA15_06880"
     CDS             complement(138..752)
                     /locus_tag="VGA15_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9587457.1"
                     /translation="MPKKKTGKTALSSAPGLRERKRQQTRERLTRVAMALFLERGFEA
                     TTLDDIAAAADISRRSFFHYFASKEDVVFTWQEESTAALIAAVAARPANESMLTAAEN
                     AISAMVRQLEPGEAMAMARLKRDNPALQARDQVKYEKLERALAEALGKRAGHKAERLQ
                     ARLVAMIATGAMRIGGELWVAEGAREKPEALVKRTFAALRAILA"
     gene            884..1822
                     /locus_tag="VGA15_06885"
     CDS             884..1822
                     /locus_tag="VGA15_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9587458.1"
                     /translation="MTDWTTADIPPLNGKTAVITGATGGLGYETALALAGAGANMVLT
                     GRNDAKGRNAVQGIRGRFPNAKASYEALDLASLASVAEFAARFVAAYPPLDLLINNAG
                     VMALPQRQTTVDGFEMQFGTNYLGHYALTARLLPSLRRGHQPRVVNLSSLAHRSGMIN
                     FDDLQSTRSYVPWKAYSQSKLAMLMFALELQRRSDAADWGLMSNAAHPGYARTDLIAN
                     GPGAIGLLWQLNKSLQPFVSHSAAEGALPTLFAATSPRAEPGGYYGPNWFYELKGPPV
                     PAKIMPRAKDPAVAARLWDVSVALTGVSFDQFAAAA"
     gene            1855..2514
                     /locus_tag="VGA15_06890"
     CDS             1855..2514
                     /locus_tag="VGA15_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HEX9587459.1"
                     /translation="MKVILFGATGMVGQGCLRECLIDPEVETVLAVGRSPTGVQNAKL
                     REIVHDNFLDFSAIESQLAGFDACFFCLGVSSVGMSEERYRHLTYDLTLAAATTLSRL
                     NPGMVFVYVTGQGTDSTEQGRLMWARIKGKTENDLLKLPFKAAYMFRPAGIQPLHGVK
                     SRTAWVHAIYVVAAPLLTWLNRVAPKYMTTTEQVGRALIKVARDGYPKPVLESEDINR
                     V"
     gene            2659..3882
                     /gene="murA"
                     /locus_tag="VGA15_06895"
     CDS             2659..3882
                     /gene="murA"
                     /locus_tag="VGA15_06895"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX9587460.1"
                     /translation="MDRIRIVGGSKLNGTIAISGAKNAALPLMIAGLLSEETLILDNV
                     PRLADVAQLQRILGNHGVDITSVGKRPGDHEYRGQTLHISAAAPLLARMHEAKVSLPG
                     GCAIGTRPVDLLIMALEKLGAELTIDGGYVIAKAPGGLKGAAIDFPKVTVSGTHVALM
                     AATLAKGTTVITNAACEPEILDVADCLNKMGARVCGAGASRIVVEGVAKLHGARHTVL
                     PDRIETGTYAMAVAMTGGHVQLSGARPELLRSALDVLTQAGAAITVNNEGIRVARNGA
                     GLQPVTISTAPFPGFPTDLQAQLMALMACAKGSSTITETIFENRFMHVQELARFGARI
                     HLDGETATIDGIDRLRGAPVMATDLRASVSLVIAGLAAEGETMVNRVYHLDRGFERLE
                     EKLSACGASIERISD"
     gene            4016..4471
                     /locus_tag="VGA15_06900"
     CDS             4016..4471
                     /locus_tag="VGA15_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEX9587461.1"
                     /translation="MTAQLRLIALDADDLAVISTHVQDARVQTADIIWKQSEKRLVVG
                     MNRLDWEQTLSGETASRRLIAALRFDRVLACKSRNIDLEAPETELELLGIEFHPGEAP
                     GGSAVLMFSQGGALRLDVECLECELTDLGTDDLGTSDFGEGAEPSGWDA"
     gene            4654..5949
                     /gene="hisD"
                     /locus_tag="VGA15_06905"
     CDS             4654..5949
                     /gene="hisD"
                     /locus_tag="VGA15_06905"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX9587462.1"
                     /translation="MPVRLDSRSADFAQQFLRFLATKREVSADIERATRAIVDDVATR
                     GDIALIEATRKFDRLDLKPGGLRITPAEIDAAVRACDIETLEALGLARDRIEIFHRRQ
                     LPKDERFTDALGVELGWRWSAIDAVGLYVPGGTAAYPSSVLMNAVPAKVAGVARVVMA
                     VPSPDGKLNPLVLAAAHLGGVSEIYRVGGAQAVAALAYGTATIAPVAKIVGPGNAYVA
                     AAKRLVFGKVGIDMIAGPSEVLVIADSTGNAGWIAADLLAQAEHDVNAQSILITDDAA
                     LADDVERAVESQLATLPRADIARASWNQFGAIIQVKKLDEAIALANAIAAEHLEIMTS
                     DPESLSAQIRNAGAIFLGPHTPEAIGDYVGGSNHVLPTARSARFSSGLGVLDFMKRTS
                     ILKCGPDQLRALGPAAMTLGTAEGLDAHARSVGLRLNPS"
     gene            5946..6449
                     /locus_tag="VGA15_06910"
     CDS             5946..6449
                     /locus_tag="VGA15_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEX9587463.1"
                     /translation="MTRPPPEEDSQNRIVAVTLDEESIGRSGPDIEHERAIAIYDLIE
                     KNLFAPEGAGEGPFALHLGITGNRLMFDIRREDGTPVVAHLLSLSPFRRIVKDYFMIC
                     DSYYQAIRTATPDRIEAIDMGRRGIHDEGSRTLQERLKGKVRVDFETARRLFTLISVL
                     HWKGQDA"
     gene            6513..>6926
                     /locus_tag="VGA15_06915"
     CDS             6513..>6926
                     /locus_tag="VGA15_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEX9587464.1"
                     /translation="MEQPSRVRTPQAVLFACGLNSVRSPMAESLLQRMFPHALYVKSA
                     GVRKGELDPFAVAVMAELGQDISGHKPTTFEELDDWEGLNFDLIITLSPEAHHRALEL
                     TRTLAADVEYWPTHDPTGAEGNREQKLQAYREVCDD"
BASE COUNT         1267 a         2174 c         2195 g         1290 t
ORIGIN      
        1 gcttttgaaa aacgggacgc tgaaaaccta caaggatttt ccgcacggca tgccgaccac
       61 ggaagctgcc acgatcaacg ccgatctgct ggcgttcatc aagggatgac atcgcgttca
      121 gtcacagatg cgggcgatca agcgaggatt gcccgcagcg cggcgaatgt tcgcttcacc
      181 agcgcttccg gcttttcgcg cgccccttcg gcgacccaga gttcaccgcc gatccgcatc
      241 gcaccggttg cgatcatggc gacaagccgg gcctgaagcc tttcggcttt atggcccgcg
      301 cgcttgccga gcgcctccgc caaggcgcgc tcaagcttct cgtatttgac ctgatcgcgt
      361 gcctgcaggg ccgggttgtc gcgcttgagc cgcgccatcg ccatcgcttc gccgggctcg
      421 agctgccgga ccatcgcgga aatcgcgttc tccgcggccg tgagcatcga ttcgttggcg
      481 ggccgcgcgg ccaccgcggc aatcagcgcc gctgtgctct cctcctgcca agtaaaaacc
      541 acgtcttcct tcgaggcaaa atagtggaag aagctgcgac gcgaaatatc tgcggcggcc
      601 gcgatgtcgt cgagcgtggt cgcctcaaag ccgcgctcga ggaacagtgc catggctacc
      661 cgagtcagcc gctcgcgggt ttgctggcgc ttgcgctccc gcagaccggg ggcgctcgac
      721 aacgcagtct tgccggtttt cttctttggt atcttagact taacctgcct gcgcacgctc
      781 accaatgatc ctcaattttt acctcttgca aatttgcact aagtgcacat ttaccaccgc
      841 gtggctgacg tccgcaaacg aatcgagcgc tggagatata agcatgaccg actggaccac
      901 tgctgacatt ccgcccctga acggcaagac cgctgtgatt accggcgcca ccggcggcct
      961 tggctatgaa accgcgctgg cgctggcagg cgccggtgcc aacatggtgc tcactgggcg
     1021 caatgacgcc aagggccgaa acgccgtcca gggaatccgc ggtcgatttc caaacgccaa
     1081 ggccagttac gaagcgctcg atctggcgag cctcgcctcg gtggccgaat tcgccgcgcg
     1141 cttcgttgcc gcttaccccc cgctcgatct tctgatcaac aatgccggcg tgatggcgct
     1201 gccgcagcgg cagacgacag tcgacggctt tgagatgcag ttcggcacca actatctcgg
     1261 ccattatgcg ctgaccgcgc gtttgcttcc ttcgcttcgc cgcggccatc agccccgggt
     1321 cgtcaatctc tccagcctcg cgcatcgctc gggcatgatc aattttgacg atctgcaaag
     1381 cacgcggtcg tacgtgccgt ggaaagctta cagtcagtcg aaactggcca tgctgatgtt
     1441 tgctcttgag cttcagcgcc gcagcgatgc agcggactgg ggattgatga gcaacgccgc
     1501 gcatcccggc tatgcgcgaa ccgatctgat tgccaatggc ccgggcgcca tcggcctgtt
     1561 atggcaactc aacaaatccc ttcagccctt tgtcagtcac tcggctgccg aaggcgcgct
     1621 gccgacgttg tttgccgcga cctcgccgcg ggcggagccg ggtggctatt atggcccgaa
     1681 ctggttctac gagttgaagg gaccgccggt gccggcgaag atcatgccgc gggcgaaaga
     1741 cccagccgtt gcggcccggc tctgggatgt ctccgtcgct ctgaccggcg tatccttcga
     1801 tcaattcgcg gccgccgctt gaatgcgtga cacaaccaag ccacgggaaa tccgatgaaa
     1861 gtcatcctgt tcggcgccac cgggatggtc gggcaggggt gcttgcgcga atgcctgatc
     1921 gatcccgagg tcgaaaccgt gctcgcggtc gggcgcagcc cgaccggcgt acaaaacgcc
     1981 aagctgcgcg aaatcgtgca cgataatttt ctggattttt cggccatcga gtctcagctc
     2041 gcgggatttg atgcgtgctt cttctgtctc ggcgtctctt cggtcggcat gagcgaggag
     2101 cgctaccggc atctgaccta cgacctcacg cttgccgcgg cgaccacgtt gtcaagactc
     2161 aatccgggca tggtgttcgt ctatgtcacc gggcaaggca ccgactcgac cgagcaaggc
     2221 cgcctgatgt gggcgcggat caagggcaaa accgaaaacg atctgctcaa gctgccgttc
     2281 aaggcggcct acatgtttcg ccccgccggc atccagccgc tgcacggcgt caaatccagg
     2341 accgcctggg ttcacgcgat ctatgtcgtg gcggcgccgt tgctgacatg gctcaatcga
     2401 gtggcgccga aatacatgac cacgaccgag caggtcggcc gcgccctgat caaggtcgcg
     2461 cgcgacggct atccgaaacc ggtgctggag agcgaggata tcaatcgggt ttagaacgaa
     2521 tcgtcattcc gggttcgagg cttcgcctcg ccccggaatg acggctagat tcccatcaaa
     2581 tagccgcaaa tttcgaagct tttgccatca ggggctcaat cgtcttatac ttgtcgcact
     2641 gcaaaaacgg gaatcggtat ggatcgcatt cgcattgtcg gcggcagcaa gctcaatggc
     2701 acgattgcga tctcgggtgc gaagaacgcg gcactgccgt tgatgatcgc agggcttttg
     2761 tccgaggaaa cgctgattct cgacaacgtg ccgcggcttg cggacgtggc gcaactgcag
     2821 cgcattctcg gcaatcatgg cgtcgatatc acctcggtgg gcaagcggcc cggcgatcac
     2881 gaatatcggg gccagacttt gcacatttcg gcggccgcgc cgctgttggc gcggatgcat
     2941 gaggcaaaag tgtcgctgcc cggtggctgc gccatcggca cccggccggt cgatctcttg
     3001 atcatggcgc tggagaagct cggcgccgaa ttgaccatcg acggcggcta tgtgatcgcg
     3061 aaggcgcccg gcggcctcaa gggcgctgcc atcgactttc ccaaggtcac cgtgagcggc
     3121 acccatgtcg cgctgatggc ggcgaccttg gccaagggca cgacggtgat caccaacgcc
     3181 gcctgcgaac cggaaatcct ggatgtcgcc gactgcctga acaagatggg tgcgcgcgtc
     3241 tgcggtgccg gcgcgtcccg catcgtcgtc gaaggtgtgg ccaagcttca cggcgcgcgg
     3301 cacaccgtgc tgcccgatcg gatcgaaacc ggcacctacg ccatggcggt ggcgatgacc
     3361 ggcggccatg tgcaactctc cggagcacgc cccgagctgc tgcggtcggc gctcgacgtg
     3421 ctgacgcagg cgggtgcggc tattaccgtc aacaacgaag gtatccgcgt cgcccgcaac
     3481 ggcgccgggc tccagccggt gacgatttcg accgcgccct ttccgggctt tccgaccgat
     3541 ctgcaggccc agctgatggc gttgatggcc tgcgccaagg gctcctctac catcaccgag
     3601 acgatcttcg aaaatcgctt catgcatgtg caggaactgg cgcggttcgg cgcgcggatt
     3661 catctcgacg gcgagaccgc caccatcgac ggcatcgatc gactgcgcgg cgcgcctgtc
     3721 atggcgacgg atctgcgcgc gtcggtgtcg ctggtgatcg cgggcctcgc cgccgaaggc
     3781 gagaccatgg tcaaccgcgt ctatcatctc gaccgcgggt ttgaacggct cgaggaaaaa
     3841 ctgtcggcgt gcggcgcttc catcgagcgc atcagcgatt gaggttgcgc gcgtattccg
     3901 caaaagcatg tcatcggaca tgttccgggg atggataccg gttttgcttc aagtttacgc
     3961 aggtggcgta gacttatctg cggtagaata cgcgcaaatt ataggtgtgc actcggtgac
     4021 ggcccagctc agattgatcg cactcgatgc cgacgacctt gccgtgattt ccacgcatgt
     4081 gcaggatgcg cgggtgcaga ccgccgacat tatctggaag cagagcgaaa agcggctggt
     4141 ggtcggcatg aaccggctgg actgggagca gacgctttcc ggggaaaccg cctcgcgccg
     4201 gctcatcgcg gcgctgcgct ttgaccgggt gttggcctgc aagtcgcgca acatcgacct
     4261 ggaggcgccg gaaaccgaac tggagctgct cggaatcgag tttcatccgg gcgaagcgcc
     4321 cggcggcagc gcggtattga tgttcagcca gggcggggcg ctgcggctgg acgtcgaatg
     4381 cctcgaatgc gaattgaccg atctgggcac cgatgatctc gggaccagcg atttcggcga
     4441 gggcgccgag ccgtccggat gggatgcctg acggcgtagc aaccgctaaa ctggtcgtcc
     4501 cggctcgcgc gtcgcttggc cgggacgacg cggattttgt tgctctcaac ccccgcacaa
     4561 tgcgtattca gtatggcaag ggttgacggc cattggccgc cgcgccattg agcagtgggt
     4621 cctcgattgc gggccgcccg caggattgcc accatgcccg ttcgactaga cagccgtagc
     4681 gccgatttcg cccagcaatt cctgcggttc ctcgcgacca agcgcgaggt atcggccgat
     4741 atcgagcgcg cgacccgcgc catcgtcgat gacgtggcta cgcgtggcga tatcgcgctg
     4801 atcgaggcga cacgcaaatt cgatcgcctt gatctcaagc cgggcggcct gcgcatcacc
     4861 ccggccgaaa tcgatgccgc ggttagggct tgcgacattg agacgctcga ggcgctcgga
     4921 ctagcccgcg accggatcga gatattccat cggcggcaat tgccaaagga cgaacgcttc
     4981 accgatgcgc tgggcgtcga gcttggctgg cgctggagcg cgatcgatgc cgtgggtctt
     5041 tatgtgccgg gtggcacggc ggcctatccg tcgtcggtgc tgatgaatgc ggtgccggcg
     5101 aaggtcgcgg gcgtcgcacg cgtggtgatg gcggtgccgt cgccggacgg caagctcaac
     5161 ccgctggtgc tggcggccgc ccatttgggc ggcgtctccg aaatctaccg cgtcggcggc
     5221 gcgcaggcgg tggcggcgtt ggcctacggc accgcgacca tcgcgcccgt tgcaaaaatc
     5281 gtcggccccg gcaacgccta tgttgccgcc gccaagcggc tggtgttcgg caaggtcggc
     5341 atcgacatga tcgccggtcc gtcggaagtg ctggtgatcg cagatagcac cggcaatgcc
     5401 ggctggatcg cggccgatct gttggcgcag gccgagcacg acgtcaatgc gcagtcgatc
     5461 ctgatcactg acgatgcagc gctcgcggac gatgtcgaac gcgcggtgga atcgcaattg
     5521 gcaaccttgc cgcgcgctga tatcgcgcgg gcttcgtgga accagttcgg cgccatcatc
     5581 caggtgaaaa aactcgacga ggcgatcgca ttggcgaacg cgatcgcggc cgagcatctc
     5641 gaaatcatga catcggatcc ggaaagtctt tcggcgcaaa tccgcaatgc cggcgcgatc
     5701 ttcctcgggc cccacacccc cgaagcgatc ggcgactatg tcggcggctc caatcacgtg
     5761 ctgccgacgg cgcgttcggc gcggttttcc tccgggctcg gcgtgctgga cttcatgaag
     5821 cggacctcga tcctgaaatg cgggccggat cagttgcggg cattgggacc ggcggcaatg
     5881 acgctgggca cggccgaagg cctcgatgcc catgcccgtt ccgtcggact gcgcctcaat
     5941 ccgtcatgac caggccgccg ccagaggagg attcgcaaaa tcgcatcgtc gcggtgacgc
     6001 tcgatgagga atcgatcggg cgttccggcc ccgatatcga acatgagcgc gcgatcgcca
     6061 tctacgacct gatcgaaaaa aatctgttcg cgccggaggg cgccggcgag gggccgtttg
     6121 ccttgcacct cggcatcacc ggcaaccggc tgatgttcga catccgccgc gaagacggca
     6181 cgccggtggt ggcgcatctg ctgtcgctgt cgccgttccg ccgaatcgtg aaggattatt
     6241 tcatgatttg cgacagctac tatcaggcga tccgcaccgc cacgccggac aggatcgagg
     6301 ccatcgatat gggccgccgc ggcattcacg acgaaggttc gcgcacactg caggagcggt
     6361 tgaaaggcaa ggtgcgggtc gatttcgaaa ccgcccgccg gctgttcacg ctgatctccg
     6421 ttctgcactg gaaggggcag gacgcatgat cccgaaaatt gggcaccggt tttcggaaca
     6481 gctcatgcgc aacacaagcg aagcctagcc gaatggagca gccttcgcgc gtgcgcactc
     6541 cgcaggccgt gctgtttgca tgcggcctga acagcgtgcg ttcgccgatg gccgaaagcc
     6601 tgctgcagcg gatgtttccc cacgcgcttt atgtcaaatc cgccggcgtc aggaagggcg
     6661 aactcgatcc gttcgcggtc gccgtgatgg cggaactcgg ccaggatata tcgggccaca
     6721 aaccgacgac gttcgaggaa ctggacgact gggaagggct caacttcgac ctgatcatta
     6781 cgctatcgcc ggaggcgcat caccgggcgc tggaactgac gcgaacgctc gccgccgacg
     6841 tggaatactg gccgacccac gatcccaccg gcgcggaggg caatcgcgag cagaagctgc
     6901 aagcctatcg cgaggtctgc gacgat
//
LOCUS       DASWGM010000227        11261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_569817,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000227 DASWGM010000000
VERSION     DASWGM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569817"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGA15_06920"
     CDS             complement(<1..368)
                     /locus_tag="VGA15_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9587465.1"
                     /translation="MNLLKSTLSAIALGSVLLAGPALAQGKPTVGIAMPTKSSARWID
                     DGNNIVKVLKERGYNTDLQYAEDDIPNQLSQIENMVTKGAKVLVIASIDGTTLSDVLK
                     QAKDKGITVIAYDRLIRDTP"
     gene            complement(452..1384)
                     /locus_tag="VGA15_06925"
     CDS             complement(452..1384)
                     /locus_tag="VGA15_06925"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9587466.1"
                     /translation="MANSRVRRPYKGVFPVVPTIFDGDGRLDLEGQKRAVDFMIDAGS
                     HGLCILANFSEQFVLTDAERDLVLDTVLSHVAGRVPVIVTTTHFASHICAERSRRAQE
                     AGAAMVMVMPPYHGATVRVGEAAIYEFYRTVSGTISIPIMIQDAPVAGTPMSAAFLAR
                     MARELENVCYFKIEVPQTAAKLRELIALGGEAIEGPWDGEEAITLMADLDAGATGAMT
                     GGGYPDGIRQIIDPYVAGRRDEAAAAYARWLPLINYENRQCGLIACKALMREGGVIRH
                     ETVRLPLQPLHPAVRAGLIEIANRLDPLVLRWGK"
     gene            complement(1458..2177)
                     /locus_tag="VGA15_06930"
     CDS             complement(1458..2177)
                     /locus_tag="VGA15_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX9587467.1"
                     /translation="MTTHLVIPAGRGHSSQLEVTRTIGIDIIVGRYPEGTGLPRDAEL
                     TSMFDVSRTVLRESVKTLSAKGLLSSKAGVGTWVRERSAWNMFDPDVLGWHLEAGIDK
                     RFLRDLADIRLAVEPRAAALAAERWTGDAIAILRDSVTRMRSFASDSIEFADADLRLH
                     LDVATISANPFMRSIGAVIEAALRASFRLSAPTEESEREMTLAAHERIVDAIADRDAE
                     AASAAMVDVIFNGLRRHGAAG"
     gene            complement(2174..3154)
                     /gene="yjfF"
                     /locus_tag="VGA15_06935"
     CDS             complement(2174..3154)
                     /gene="yjfF"
                     /locus_tag="VGA15_06935"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HEX9587468.1"
                     /translation="MRRLLPVLVTTAVFVIGFILCSLQFPNFASTRVVANLLTDNAFL
                     GIVAVGMTFVIISGGIDLSVGSVIGFTTVFLAIAIERYGIPPFVAFAIVLVICLLFGA
                     AMGAVIHYFEMPAFIVTLAGMFLARGAAFLMSTESIPINAPLYSDIGLLTIYLPGGGR
                     LPLIAMIMLVVFAMGAVLLHFTRFGTNVYALGGSRYSTALMGVRVAPTIVRVYVLSSV
                     LAGLSGIVFSFYTAAGYALSAVGVELDTIAAVVIGGTLLTGGAGFIFGTLMGLLIQGL
                     IQTYITFDGNLSSWWTKIVTGLLLFAFIAFQQTLVRLAKRPATLAAGGTS"
     gene            complement(3154..4128)
                     /locus_tag="VGA15_06940"
     CDS             complement(3154..4128)
                     /locus_tag="VGA15_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9587469.1"
                     /translation="MLRIGTPQSLAFLTILMADRLVSPQFFDIRLQDGRLFGSLVDVF
                     NRGAPVALLSLGMVLVIATRGIDLSVGAVMAIAGAIAASLVDSHSVPIALGAALGVGL
                     LCGLWNGFLVAVLRIQPIVATLILMVAGRGIAQLITEGRIVTFSSPSLAWFGNGSVVG
                     IPAPVAITLGMLLLTLLLVRGTALGVLIEAVGANARASELAGIGTQAITITAYVWCGL
                     CSAVAGVISAADILGADANNSGLWLELDAILAVVVGGTSLFGGRFSLILAVLGALIIQ
                     AMNTGILLSGYRPELNLVVKAIVVIAVLLLQSPRLAGFGYWFNRKGTT"
     gene            complement(4156..5685)
                     /locus_tag="VGA15_06945"
     CDS             complement(4156..5685)
                     /locus_tag="VGA15_06945"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX9587470.1"
                     /translation="MASRPELARPLLEVAGLTKSFLRQRVLNGVDLTLHSGEIHALLG
                     ENGAGKSTLIKAVTGVVQRDAGTVKLDGVEIMPRDAEEAVKLGIATVYQEVNLLPNLT
                     VAENLFLGRQPTRFGLVRDREMRRNAAILLRGFGLAIDVGAPLGDYSVAVQHLVAIAR
                     AVDMSARVLILDEPTASLDAHEVDILFGIMRQLAARGIGILFVTHFLDQVYAISDRIT
                     VLRNGQLVGRRETAALPRLDLVHMMLGRELSEVTADREHRPIDESKPPYALFQHFGKR
                     RYLAPFDLALRDGQVVALAGLLGSGRTETARMVFGVERADTGTVQVAGEKVRVGSPRD
                     AVGLGFGYCPEERKTEGIIAELSVRENIVLALQARRGWMKPLSRREQDEIARHFITLL
                     DIRPADPERPIGLLSGGNQQKALLARWLATKPRLLILDEPTRGIDVGAHAEIIRLIRQ
                     LCADGLALLVISSEIEEVVTYADEVIVLRDRAHVMTLSGDAITVPAVLATIAAETPHV
                     A"
     gene            complement(5758..6726)
                     /gene="ytfQ"
                     /locus_tag="VGA15_06950"
     CDS             complement(5758..6726)
                     /gene="ytfQ"
                     /locus_tag="VGA15_06950"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter,
                     galactofuranose-binding protein YtfQ"
                     /protein_id="HEX9587471.1"
                     /translation="MTLRKSLVAALAALTPFVATSAVAASLTVGFSQIGSESGWRAAE
                     TSVSKSEAQKRNITLKIADAQQKQENQIKAIRSFVAQGVDAIFLAPVVATGWDAVLNE
                     AKDAKIPVILLDRDIETANKSLYLTAVTSDSVHEGVVAGEWLAKNVGSKPCNVVELQG
                     TVGASVATNRKKGFDEAIAKHSNIKMIRSQTGDFTRAKGKEVTESFLKAENGGKNICA
                     LYAHNDDMAVGAIQAIKEAGLKPGKDILVVSIDAVPDIFKAFMVGEANATVELTPNMA
                     GPAFDALIAFKEKGTVPPKWIQTDSKLYTPTDDPQKIYDSKKGLGY"
     gene            complement(7402..7755)
                     /locus_tag="VGA15_06955"
     CDS             complement(7402..7755)
                     /locus_tag="VGA15_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HEX9587472.1"
                     /translation="MIEPKLEVPAELRELAEKTIDQAEKAFEMFFDAAGKSMASVPTP
                     GTEISKQALSFTEQNLKAAFEHARKLVHATDLQEALQIQSEFLKSQFTNAGEHMRQIT
                     GGVMSAAKDASKGKF"
     gene            complement(7920..8141)
                     /locus_tag="VGA15_06960"
     CDS             complement(7920..8141)
                     /locus_tag="VGA15_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587473.1"
                     /translation="MKDFSNASFSPELIKAMDEALEGAVATLPHPVSSTRVRNIAESI
                     LRSAKQGESNPRTLQMMALVEMQIRSGEQ"
     gene            8404..8625
                     /locus_tag="VGA15_06965"
     CDS             8404..8625
                     /locus_tag="VGA15_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587474.1"
                     /translation="MAFYYRPDWIKWGLRAATNGIEAVGDALASPWGDRIEIVLRELG
                     GFIWIQIIFIRVVFSSVATGWRYRRRNDG"
     gene            9032..9274
                     /locus_tag="VGA15_06970"
     CDS             9032..9274
                     /locus_tag="VGA15_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587475.1"
                     /translation="MLPWYASAMLILESSSVVGLRLTKLTRGGSEAGDEANLMVTEKI
                     TAAFEAVETLLTGGGLSIVIDRYRKHVTANATRLAG"
     gene            9284..9772
                     /locus_tag="VGA15_06975"
     CDS             9284..9772
                     /locus_tag="VGA15_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587476.1"
                     /translation="MPAITRKRRWKMTTLLAARRHIRRLGPYQSLALMLLPILLVEPL
                     KIVALFVVGHGHWLTGTGMLVAAYGVSLVVVERLFKVVKFKLMTVNWFADLWRWFTAM
                     RDRMFGRQPGQAVGQRVDSTDVPFSEGGSAHAEARLIGLRRGSTSADLSHRPHRSPWK
                     SG"
     gene            complement(10087..10563)
                     /locus_tag="VGA15_06980"
     CDS             complement(10087..10563)
                     /locus_tag="VGA15_06980"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9587477.1"
                     /translation="MAVHLMQKQTFRRATSADASTVREITRAAYSKWIPVIGREPKPM
                     TANYLQAVADHVIDLFEEAGRPIALIEVIPKPSHLLIENIAVLPDQHGKGIGGLLLKR
                     AETIARSLGLNELRLYTNAMFAANITFYAHRGFEEFLREPHAAGGEVVHMKRAVEP"
     gene            10749..>11261
                     /locus_tag="VGA15_06985"
     CDS             10749..>11261
                     /locus_tag="VGA15_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX9587478.1"
                     /translation="MPTAALVSLATSAPPHVFLQKDVLAAAWDELGPRFPEFATFSSI
                     FANTGIVKRHGVRPFEWYLKPRGWPERTQAFLEGAEALFVDVAQKALASAHLGGGDID
                     TVVTVCSTGIATPSLEARVARAMGFRSDVMRVPVFGLGCAGGVSGLSIASRLAQARPG
                     TNVLLVAIELC"
BASE COUNT         2255 a         3490 c         3378 g         2138 t
ORIGIN      
        1 ttgggcgtgt cgcggatcag ccggtcgtag gcgatcaccg taatgccctt gtccttggcc
       61 tgcttgagca catcggacag ggtggtgccg tcgatcgaag cgatgaccag caccttggcg
      121 cccttcgtca ccatgttctc gatctgggag agctggttcg gaatatcgtc ttcggcatat
      181 tgcaggtcgg tgttgtaacc gcgttccttc agcaccttca cgatgttgtt gccatcatcg
      241 atccagcgcg ccgaggattt ggtcggcatg gcgatgccga cggtcggctt cccctgcgcg
      301 agggcgggtc ccgcaagtaa gacggaaccg agcgcgatgg cggatagagt cgatttgagt
      361 agattcatgt ttcactccca agtgttgagc ctcagtcgcc gcgttgtcgc ggtttgcatt
      421 tggcgccggt acgtatcggc gcgtcgtcgg tttatttgcc ccatcgcagt accagcggat
      481 cgaggcggtt ggcgatttcg atcaacccgg cccgcacggc cggatgtaac ggctgaaggg
      541 gaagacggac ggtctcgtgc ctgatcactc ccccttccct cataagcgcc ttgcaagcga
      601 taaggccaca ctggcggttc tcgtagttga tcagcggcag ccatcgcgca taggccgcgg
      661 cggcttcgtc gcggcggccg gcgacatacg gatcgatgat ctggcggatg ccgtcgggat
      721 aacctccgcc agtcatcgcc ccggtcgcac cggcatcaag gtcggccatc aacgtgatgg
      781 cctcctcgcc gtcccacggg ccttcgatcg cctcaccgcc gagcgcaatc aactcgcgca
      841 gcttggcggc ggtctggggc acctcgatct tgaagtagca gacgttttcc agctcgcgcg
      901 ccatgcgggc gaggaaggcc gccgacatcg gcgtgccggc gaccggcgcg tcctggatca
      961 tgatgggaat cgagatcgta cccgaaacgg ttcggtagaa ctcgtagatt gcggcttccc
     1021 cgacccgtac ggtcgcgccg tgatagggcg gcatcaccat caccatggca gcgccggcct
     1081 cctgtgcgcg ccttgaacgc tcggcgcaga tgtgggaagc gaaatgcgtg gtcgtgacga
     1141 tcaccggtac gcggcccgcg acatggctca agaccgtgtc gagtacaagg tcgcgctcag
     1201 cgtcggtcag cacgaactgc tcggagaaat tggcgaggat gcagagcccg tgcgagccgg
     1261 cgtcgatcat gaagtcgacg gcgcgcttct gaccctcgag atcaagccgg ccgtcgccgt
     1321 cgaatatcgt cggcaccacc gggaatacgc ccttgtaggg ccgccggact ctggagtttg
     1381 ccataaccat cccctgcttt cgtttccagc tcttgccagc tctcgccgta cgatgatcgc
     1441 gccctgctcc gtggctttca gccggccgca ccatgccggc gcaggccgtt gaaaataaca
     1501 tccaccatcg ccgcggaggc tgcttctgca tcgcggtcgg cgatcgcatc gacgatgcgc
     1561 tcgtgggcag cgagagtcat ctcgcgctcg gattcttccg tgggagcgct gagccgaaac
     1621 gatgcccgca atgccgcttc aatcacggcc ccgatcgacc gcatgaaagg gttggccgag
     1681 atcgtcgcga catcaagatg caggcgaaga tcggcatcgg cgaattcgat gctgtctgaa
     1741 gcgaaacttc gcatcctggt gacgctgtcg cgcagaatcg cgatggcatc gcccgtccac
     1801 cgttcggcgg cgagcgcggc cgcacgtggc tccaccgcaa ggcgaatatc ggcgagatcg
     1861 cgaagaaacc gtttgtcgat cccggcctcg agatgccagc cgagcacgtc cgggtcgaac
     1921 atgttccagg ccgagcgctc cctgacccag gtgccgacac ccgccttcga gctcagcagc
     1981 cccttggccg acagcgtctt gacgctttcg cgcagcacgg ttcgcgacac gtcgaacatc
     2041 gaggtcagtt ccgcatctcg cggcagaccg gttccttccg gatagcggcc tacgatgatg
     2101 tcgatcccga tcgtccgggt tacttccagc tgactggaat ggccacggcc tgccggaatt
     2161 accagatggg tggtcatgac gttcctcccg cggctagtgt ggcaggccgt ttggcgaggc
     2221 gcaccagggt ctgctggaaa gcgatgaagg caaacagcaa aaggcccgtc acgatcttgg
     2281 tccaccagct cgacagattg ccatcgaaag tgatgtaggt ctggatcagg ccctggatca
     2341 gaagccccat cagcgtgccg aatatgaagc ccgcgccgcc ggtcaacagc gtaccgccga
     2401 tgacgacggc ggcaatggtg tcgagttcca cgccgacggc agacagcgcg taaccggcgg
     2461 cggtgtagaa ggaaaacacg atacccgaca ggccggccag cacgctggac agcacgtaga
     2521 cccgaacgat tgtcggcgcc acacgaacgc ccatcagcgc cgtcgaatac cggcttccgc
     2581 cgagcgcgta gacattggta ccgaagcggg taaaatgcag cagcacggcg cccatcgcaa
     2641 acacgacgag catgatcatc gcaatcaggg gcagccggcc gccgcccggc agatagatcg
     2701 tgagcaagcc gatatcgctg tagagcggtg cattgatcgg gatcgactcc gtcgacatca
     2761 aaaaggctgc gccgcgcgcc aggaacatgc cagccagggt gacgatgaag gctggcattt
     2821 cgaagtagtg aatcaccgcg cccatcgccg cgccgaacag caggcagatg accaggacga
     2881 tggcgaaggc aacgaagggc ggaattccat agcgctcgat cgcgatggcg agaaacacgg
     2941 tggtgaagcc gataaccgac ccgaccgaaa ggtcgatccc gccggagatg atgacgaagg
     3001 tcatgccgac ggcaacaatg ccgagaaagg cattgtcggt gagaagattg gcgacgaccc
     3061 gcgtggacgc gaagttcggg aactgcagcg agcagagaat gaacccgata acaaagacag
     3121 cggtggtgac cagaacggga agcagacggc gcatcaggtc gtccccttcc tgttgaacca
     3181 ataaccgaaa ccggcgagac gcggcgactg cagcagcaga accgcgatca caacgatggc
     3241 cttcaccacc aaattgagct ccggacgata gccgctgagc aggataccgg tgttcatggc
     3301 ctggatgatc aacgcaccga gcacggcgag tatcaggctg aaacgcccgc cgaacaagga
     3361 ggtgccgccg accaccacgg caaggatggc atcgagctcg agccacagac cggaattgtt
     3421 ggcatcggcg ccaagaatgt cggcggcgga aatgacaccg gcgaccgccg agcaaagacc
     3481 gcaccagaca taggcggtga tggttatcgc ctgcgtgcca atgccggcaa gttcgctggc
     3541 gcgggcattg gcgccgaccg cctcgatcag cacgccgaga gccgtgcctc gaaccagcaa
     3601 caaggtcaga agcagcatcc ctagggtgat ggcgaccggc gcgggaatgc caacaaccga
     3661 tccgttgccg aaccaggcaa gcgacggcga agagaaggtc acgatcctcc cctcggtgat
     3721 gagctgggcg atgcctcgac cggccaccat caggatcaag gtggcgacga tcggctggat
     3781 ccggagcacc gccaccagaa agccgttcca gaggccgcac agcaacccga caccgagggc
     3841 cgcgccgagc gcaatcggca cggagtggct gtcgaccaga ctggcggcga tggcacccgc
     3901 gatggccatt accgcgccga ccgacagatc gatgccgcgc gtcgcgatca ccagcaccat
     3961 gcccagcgac agaagcgcca ccggcgcgcc gcggttgaaa acatcgacga gactgccgaa
     4021 caggcgcccg tcctgcaggc ggatgtcgaa gaactgcggc gaaaccagcc gatccgccat
     4081 caggatggtc agaaaggcca ggctttgcgg agtgcctatc cttagcatgc gaaacgactt
     4141 cacggtcatc tttcctcagg ccacgtgggg cgtttcggcg gcgatcgtcg ccagcaccgc
     4201 cggcaccgtg atggcgtcgc cgctcaaggt catgacgtgc gcgcggtccc gcagcacgat
     4261 gacctcgtcc gcataagtca cgacctcctc gatctccgag gagatgacaa gaagggcaag
     4321 cccgtcggcg caaagctggc ggatcaggcg tatgatctcg gcatgcgcgc cgacgtcgat
     4381 gccacgggtc ggctcgtcga ggatcagcaa ccgcggcttg gtggccaacc agcgtgcaag
     4441 cagggccttc tgctggttgc cgcccgacaa caggccgatc ggccgctccg gatctgcggg
     4501 acggatatcc agcagcgtga tgaagtgccg cgcgatctcg tcctgctccc gcctggagag
     4561 cggcttcatc cagccacgcc gcgcctgaag ggcgagcacg atgttctccc gcacggaaag
     4621 ttcggcaatg atgccctccg tcttgcgctc ttccggacaa tagccgaacc caaggccaac
     4681 agcatcgcgc ggcgagccga cgcggacttt ctcgccggca acctgcaccg taccggtgtc
     4741 ggcacgttca acgccgaaca ccatccgcgc cgtctcagtt cgacccgaac cgagcaaacc
     4801 agcgagcgcc acgacctggc cgtccctcaa tgccagatcg aacggcgcca aatagcgccg
     4861 cttgccgaaa tgctggaaaa gcgcgtaagg cggcttggac tcgtcgatcg gcctgtgttc
     4921 ccgatcggcg gtcacctctg acagctctct gcccagcatc atgtggacaa ggtcgagccg
     4981 cggaagcgcc gccgtttcac gccgaccgac cagctggccg ttccgcagca cggtgatgcg
     5041 atcactgatg gcgtagacct gatcgaggaa gtgcgtaacg aacagaatgc caatgccgcg
     5101 agcggcgagc tggcgcatga tgccaaacag aatgtcgacc tcgtgcgcat caagactcgc
     5161 ggtgggctcg tccaggatca gcacgcgcgc ggacatgtcg acggcccggg cgatcgcaac
     5221 aagatgttga acagcgaccg aatagtcgcc gagcggagcg ccgacgtcga tggcgagccc
     5281 gaagccgcgc aggagaatag ccgcgttgcg gcgcatttcg cgatcgcgca ccagcccgaa
     5341 ccgtgtcggc tgccggccca ggaacaggtt ttctgcaacc gtgagattgg gcaggagatt
     5401 gacctcctga tagaccgtgg cgatgccgag cttcaccgcc tcctcggcat cgcgcggcat
     5461 gatttccacg ccgtcaagct tcaccgtgcc ggcgtcgcgc tgaacaaccc cggtgactgc
     5521 cttgatcagc gtcgacttgc cggcgccgtt ttcacccagc aaagcgtgaa tctcgccgga
     5581 atgcagcgtc agatccacgc cattaagaac acgttgtctc aagaagctct tggtcaagcc
     5641 cgcgacttcg agcagggggc gagcaagttc cggacgcgaa gccatcgtcg acactttcca
     5701 acaggacgca gtgagaacgg ccgggcaagg cccggccgcg cccggtccat ggctcaatca
     5761 gtagccaaga cccttcttgc tgtcatagat cttctgcgga tcgtcggtcg gcgtatagag
     5821 cttcgaatcc gtctggatcc atttcggcgg caccgttccc ttttccttga acgcaatcag
     5881 cgcatcgaag gcaggtcccg ccatgttcgg cgtcaactcg accgtcgcgt tcgcctcgcc
     5941 caccatgaac gccttgaaga tgtcgggcac cgcgtcgatc gaaacaacga ggatgtcctt
     6001 gccgggcttg aggcctgctt ccttgatcgc ctgaatcgcg ccgacggcca tgtcgtcgtt
     6061 atgggcatag agcgcgcaga tattcttgcc gccgttttcg gccttcagga agctttcggt
     6121 gacttccttg cctttggcgc gggtgaaatc gccggtctgg ctccggatca tcttgatgtt
     6181 ggaatgcttt gcgatcgcct catcaaaccc cttcttgcgg ttggtggcga cgctcgcccc
     6241 gacggtgccc tgcagctcga ccacgttgca tggcttgctg ccgacatttt tcgccagcca
     6301 ctcaccggcg accacgcctt catgaacgct gtcggatgtg acggcggtca aatagagcga
     6361 tttgttcgcg gtctcgatgt cacgatcgag caggatgacc ggaatcttgg cgtccttggc
     6421 ctcgttgagc acggcatccc agccggtcgc aacgacaggc gcgaggaaaa tggcgtcaac
     6481 gccctgcgcg acgaaagagc gaatggcctt gatctggttc tcctgcttct gctgagcgtc
     6541 ggcgatcttc aatgtgatgt tgcgcttttg cgcttcgctt ttggacacgg aagtttccgc
     6601 cgcgcgccaa cctgattccg agccgatctg cgagaagcct accgtcagac tggcggcgac
     6661 ggcgctggtg gcgacaaacg gagtgagcgc ggcgagcgcg gcgacaagag actttctgag
     6721 agtcatgagg tttcgttccc tgattgttga gccgccgtcc tgcttcttcg agcaagatca
     6781 aagctggcgg catacttagt gccacaatgc ccaaagcctg tataggcggt ttgtattatt
     6841 agtatgacta atgcccgtcg tgcagaggcg aaatgatcgg gcattcccgg atagcagatg
     6901 atttcgctac aatcccaaag agttagcgat gcgggccttg cgcggtcgac ggcttcaccg
     6961 acgccgacga tgaaagccaa tacgacgaac gccgccgaaa tcgagcgctg gtattcacga
     7021 acttcccaag gtgtcaccgg cacggttcgc gcgagatttc cggctcacgt tcggggagct
     7081 ggcatccgag accttgaaga gttcagccag ccggccgctt tcttgatcgc gaccccgctc
     7141 caactgctcg gatccgttcg cggcccaaat gcaaatcccc gccggttttg gccgacaggg
     7201 atcagcagaa aagttgacag acggcgagtg gcatcacccg gtggcccgtc aaggacctgc
     7261 tcaccaattg aagtctaccc tacaaatcca tgcaagacta ttttgtgggc agattcatca
     7321 tctgcgtaca cctggcgtta caccacggcc gatgctctcc gatcgccgat ggaaggcatt
     7381 atcgccgccc cactaccgct cctagaactt gcctttcgac gcatccttcg cagccgacat
     7441 gaccccgccg gtgatctgcc tcatatgttc tccggcattg gtaaactggc tcttcaagaa
     7501 ctccgattga atttgcaggg cttcctgaag atcggtcgcg tgaaccagct tgcgcgcatg
     7561 ttcgaacgca gccttcaagt tctgttcggt gaacgacagc gcctgcttgg aaatctccgt
     7621 ccccggggtc gggaccgacg ccatcgactt gcccgcagca tcaaaaaaca tctcgaacgc
     7681 tttttccgcc tgatcgatgg ttttttcggc caactcgcgc aattcggccg ggacttcgag
     7741 tttcggttca atcatgccgt catcctctgg tttgaacgga gcgttaccac atttccagaa
     7801 cggcgtcttc gcgctgtttg cctcgggcgg ttttggtatt accgacccct ttttggaaat
     7861 tcagcactca tcgtcgggat gtacccagac ccaaggcgcg gtcccccaat cggctcggat
     7921 cattgctcgc cggatcggat ctgcatttcg accaaggcca tcatttgcaa tgtcctgggg
     7981 ttactttcgc cctgcttcgc actccgaaga atgctctccg cgatgtttcg cacgcgcgtc
     8041 gagcttaccg ggtgtggcag cgtggctacc gccccctcca gcgcttcatc cattgccttg
     8101 atcagctcgg gagaaaacga ggcattcgaa aaatctttca tccgctgcgt ccgtttccga
     8161 tccggatgga tcgtctgcaa agacaagccg ccggcctacc gcatgttccc aacttctatc
     8221 gggtggcgga acaaatcaag cgcggcggtc attatccctc gtcgatagga gatgccccca
     8281 atgcgccgcg ccccaaccga tccgcgacaa gatcgatctg tctgaccccg cgcaaatgcg
     8341 cgcccggtca aagtacacag ttttcgaatc atcaccgcta tcctggcatg ggttttcatc
     8401 ggagtggcgt tttattaccg gccggactgg atcaagtggg ggctgcgagc tgcgacgaat
     8461 ggcatagagg cggtcggcga cgctctggct tccccctggg gcgatcgcat cgaaatcgtt
     8521 ctcagagagc tcggcgggtt catctggatt cagatcatct tcattcgcgt ggtcttttcg
     8581 agcgtcgcga cgggctggcg gtaccgaaga cgcaacgatg gttgaagccc cgagcacgga
     8641 cggccgacgc gccgagtcca tgcaaaggga actccggcac aaacggaacc ccggctcggt
     8701 ttcgcggttg ttgattgatg aaaagtgatc cttttgagtc tgccatagat gaaatcctgg
     8761 cgagtgccga tggcgacgtg cggcgcgcat tacgtgccgt gcttgttgaa aatatcgagc
     8821 tggaatcgga actccgaagc ctgtaggcgg cgtcggagca tggcaagacc accgacacca
     8881 agaactccct gcactaactg cgcgataacc accacacgcc accatcgcaa catatccgga
     8941 tgagatatgc ccgtgccgag gatccgacgt cggaagcatc gggtaagcat gctgttgtgc
     9001 tcaggcagct tggcctgcat gaggaaaaga catgttgcca tggtatgcgt cggcgatgct
     9061 gatcctggag tcgagcagcg tcgtgggcct gcgactgacg aaactcacgc gcggaggcag
     9121 cgaggccggt gacgaggcta atttgatggt caccgagaaa attaccgcag cattcgaagc
     9181 tgtcgaaacc ctgttgactg gtggcggcct ctccatcgtt attgaccgct atcgtaaaca
     9241 tgtcacggcg aatgcaactc gtctcgcggg ttaggaccga gccatgccgg cgatcacccg
     9301 caaacgccga tggaagatga ctacactgct ggcggcccgt cgccacattc ggcgattggg
     9361 accttaccag tcgcttgcat tgatgctttt gccgatcctg cttgtcgagc cgctgaagat
     9421 tgtggccctg tttgttgtgg gccacggaca ttggctgacc gggaccggaa tgctggttgc
     9481 ggcctacgga gtcagtctcg tggtcgttga acgacttttc aaggtggtca aattcaagct
     9541 gatgaccgtg aactggttcg ccgatctgtg gagatggttc accgccatgc gcgacaggat
     9601 gttcggacgc caaccgggcc aagcggtcgg gcaacgcgtc gacagcaccg atgtcccgtt
     9661 ctctgaagga ggctctgctc atgctgaagc cagacttatc ggcttgcgca gaggctcaac
     9721 ctctgccgat ctctcgcatc gcccacatcg aagtccatgg aagtcaggct aggatttttg
     9781 gcccggcggg tcgccgcccc tgtcctgctg gctgacctaa tgggcgcggg ctttgtcggg
     9841 cggctgctcc tgtccccgct cgtcgcgcac aatgccttgg tccttgcccc cccgatggac
     9901 gcggctccga cctgggcatg cccttctgac cgccatgttt gccgccctgc gtcggcaatg
     9961 tgggaccggg cgctgccgat ttcaaaatga agtccggaaa gatgaaatct ggcacgacca
    10021 ccggaatgaa tccaggagcc cacaagggga gagagtgcgg ccaaaaaaac aaaggagttt
    10081 gaccgcctaa ggctcgaccg ctctcttcat gtgaaccacc tcgccacccg ccgcatgcgg
    10141 ctcacgcagg aattcttcaa atccgcgatg cgcatagaat gttatgttcg cggcaaacat
    10201 cgcattggta tacaatctca actcattcaa tccgagtgat cgcgcaatgg tctcggcgcg
    10261 cttgagaagc aagcccccaa tgcctttgcc gtgctgatcc ggcaggacgg cgatattctc
    10321 gatcagcaga tgcgaaggct tggggatcac ctcaatcagc gcaattggcc ggccagcttc
    10381 ttcaaagaga tcgatgacat ggtcggccac ggcctgcagg taattcgccg tcattggctt
    10441 cggttctcgg ccgatcaccg gaatccactt tgagtaggcg gcccgcgtga tttcacgaac
    10501 agtgcttgcg tccgcggatg tcgctcgcct gaaagtctgt ttctgcatca ggtgcaccgc
    10561 catcgccgac aaatcgcctc aatgctaatt gatcacagtc caagcctcca gaccaaatct
    10621 cagaggctgc acaggcgacg tgtttcacgt tttggtgccc cattcgacct gatggtctgc
    10681 accggcactg gaataaacga cccgcgccga tgtttcccat aaactgaagc agcaggagag
    10741 atctcgcgat gcccacggct gccctggttt cgctggcgac atcggcccct cctcatgtat
    10801 tcttgcagaa ggatgttctc gccgcggcct gggatgaact cggaccccgc tttcctgaat
    10861 tcgcgacgtt ttcgagtatc tttgcgaaca ccggaatcgt caaacgtcac ggcgtcaggc
    10921 cgttcgagtg gtatctcaag ccgcgcggct ggccggagcg tacccaggca tttctggagg
    10981 gggccgaggc gctgttcgtc gatgtcgcgc agaaggcgct ggcgagcgca caccttggcg
    11041 gcggcgacat cgacaccgtc gtgaccgttt gctccaccgg tatcgcaacc ccgagtctcg
    11101 aagcgcgcgt cgcccgcgcg atgggttttc gctccgacgt catgcgcgtg ccggtgttcg
    11161 gcctcggctg cgcgggcggc gtttccggcc tgtcgattgc ctcgcgcctt gcgcaggcgc
    11221 ggccgggtac caacgtgctg ctggtcgcga tcgagctatg c
//
LOCUS       DASWGM010000228         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1820378,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000228 DASWGM010000000
VERSION     DASWGM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820378"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VGA15_06990"
     CDS             complement(<1..759)
                     /locus_tag="VGA15_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587479.1"
                     /translation="MRRLSVSNTVLLIICLMYLILYVDRVNISTAAPLIKAELNLSNT
                     QLGLAFSAFAYPYALFQLIGGYFGDKFDARRTLFVSGLIVCAATAATGIVGGLASLFA
                     ARLALGIGEGATFPTATRAMAAWVPERSWGFAQGITHSSARIGNAVTPPLIAALVGLV
                     SWRGAFVVLAFLSLGWVLVWLWYFRDIPTAPPLTAKELATLPVRSRGDATEPVPWFRL
                     FRRMLPVTAVDFCYGWTLWLFLSWIPSFFYQNYQQ"
     gene            complement(759..>1585)
                     /locus_tag="VGA15_06995"
     CDS             complement(759..>1585)
                     /locus_tag="VGA15_06995"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX9587480.1"
                     /translation="TPLGAEVRGIDLRDLDASQFAALERAWHDHQVLLVRGQTLSDGE
                     LIAFSRRFGDLDWAPIQETGRRFVEGLPEIYIVSNVTVNGQPIGSLGAGEAVWHTDMS
                     YLDVPPMASMLYALEVPPAGGNTSFCSMYAVYEALPDQLKRRIADLKIKHDGTYNSGG
                     YLRQGVTATDDPLVSPGAVHPLVCTHPDTGRRMLYLGRRRNAYLVGLELADSEALLDE
                     LWDFVARPEFAWEHVWQVGDLVLWDNRCTMHRRDPFDASTRRIMHRTQIKGSAAPA"
BASE COUNT          315 a          505 c          511 g          254 t
ORIGIN      
        1 ctgctgatag ttctggtaaa agaacgacgg tatccagctc aggaacagcc acagcgtcca
       61 gccgtagcaa aaatcgaccg cggtcaccgg cagcatgcgg cgaaataatc tgaaccacgg
      121 caccggctcg gtcgcatcgc cccggctgcg cacggggagt gtcgcaagtt ccttcgcggt
      181 cagcggcggc gctgtcggta tgtcgcgaaa gtaccacaac cagaccagga cccagccaag
      241 gctcaggaat gccaggacaa cgaacgcacc acgccacgat accagcccaa ccagcgcggc
      301 gatcagaggc ggcgttaccg cgtttccgat ccgcgcagac gaatgcgtga tgccctgcgc
      361 gaaaccccaa ctgcgctccg gcacccatgc cgccatcgcg cgggtcgcgg tcggaaaggt
      421 cgcgccttcg ccgatcccca gagcaagcct tgctgcgaac aggctcgcaa gcccgccgac
      481 gatgccggtt gccgccgtcg cggcgcagac gataagaccg ctgacgaaca gcgtccgacg
      541 tgcatcgaat ttgtcgccaa agtagcctcc gatcagctgg aacagcgcat acggataggc
      601 gaatgccgaa aaagccaggc cgagctgcgt gttgctgagg ttaagctccg ccttgatcag
      661 tggcgcggcg gtggaaatgt tgacccgatc cacgtagagg atcaggtaca tgaggcagat
      721 gatcaacagg accgtgttgg aaacgctcag tctgcgcatc aggccggcgc cgcgctgccc
      781 ttgatctgcg ttcggtgcat gatgcggcgt gtcgaggcgt cgaacgggtc gcgccgatgc
      841 atggtacagc ggttgtccca cagcacgagg tcgccgacct gccagacgtg ctcccaggcg
      901 aactccggcc gcgcgacgaa atcccagagc tcatcgagca aggcctcgga gtcggccagt
      961 tcgagcccca cgagatacgc attgcgtcgg cggccgagat aaagcatgcg ccgcccggtg
     1021 tcggggtggg tgcagaccag cgggtgcacc gcgcccggtg acaccagcgg gtcgtcggtc
     1081 gcggtgacgc cctggcgcag ataaccgccg ctgttgtagg taccgtcgtg cttgatcttg
     1141 aggtccgcga tccggcgctt cagttggtcc ggcagtgctt cgtagacagc gtacatgctg
     1201 caaaacgagg tgttgccacc ggcaggcggc acttcgagag cgtaaagcat gctcgccatc
     1261 ggcggaacat cgagatacga catgtcggtg tgccatacgg cttcgccggc gccgaggctg
     1321 ccgatcggct ggccgttcac ggtcacgttt gaaacgatgt agatttcagg cagcccctcg
     1381 acgaagcgcc gtccggtttc ctgtatcggg gcccagtcga ggtcgccgaa gcggcggctg
     1441 aatgcgatga gctcaccgtc gctgagcgtc tggccacgca ccaacagcac ctgatggtcg
     1501 tgccaggcgc gctcgagcgc cgcgaactgc gacgcatcga gatctctgag atcgatgccg
     1561 cgcacctcgg cgccgagtgg cgtcc
//
LOCUS       DASWGM010000229         8942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_985637,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000229 DASWGM010000000
VERSION     DASWGM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985637"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1654
                     /locus_tag="VGA15_07000"
     CDS             26..1654
                     /locus_tag="VGA15_07000"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX9587481.1"
                     /translation="MTKGSDLLVAALENEGVQCIFAIPGEENLDVLESLRRSKIKLVL
                     TRHEQAAGFMAATYGRLTGRAGVCLTTLGPGALNLTTAAAYAHLGAMPMVMITGQKAI
                     RSSRQARFQIVDIVATMRPLTKMATQIVSAQSIPTLVRDAFRVAQQERPGPVHLELPE
                     DIAADKAAADIVPRHVVEIPIAPAAAIERAAAMIMAAKRPLVMLGAAASRPHLAGPLS
                     AFVRRAKIPFFNTQMGKGAVNAGSNLYIGTAALSERDWVHEAIDQADLIVSIGHDTIE
                     KPPFLMGHDGPQVIHVGATPATVEQVYFPQAEIVGDVGASLAGLADRLEGKLPNASAL
                     LGLREGILAHLAEGSTEDRFPLTPQRIVHDVRKVIPPDGIVALDNGMYKIWFARNYRT
                     SVANTLLLDNALATMGAGLPSAIAASLINPDKRVLAVCGDGGFMMNSQELETAVRLKQ
                     NLVVLVLEDHAYGMIRWKQEVDGFSDFGLDFGNPDFVAYAKSYGAKGSRVGNAAEFAT
                     MLETAFREGGVHLVVAPIDYSENMRVLVHELHSV"
     gene            complement(1674..3758)
                     /locus_tag="VGA15_07005"
     CDS             complement(1674..3758)
                     /locus_tag="VGA15_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX9587482.1"
                     /translation="MKIGTLLTVAIVSLSAVGGGLAVYVAATKYQTMDKISVAQRRLE
                     IVRAVGDIPRYMNPERGFATNIMFGPSTVDPALRAELNDKYRKQTDGAREKMNQIKAT
                     LPGTLEDSAGVASGIDALNVKFAALRETIDKALDGPPEARRDGAKKAVADNVAFNTAV
                     TVLLDEQVRKMALLDGGAYRQASYANIAWTLRDVGGTNASLYKNLVAAKRVATDAEKL
                     DLSRSQGRNDQILLSLQELRGNPATPANVAAALTKMNEAYVERFGQEQKLVKDGAVSG
                     KYEHDVETYYAESQRGLGAIIEVREAFYDNAEHILSAAYSDARFSFVVALAGLAAVAS
                     ASVGLVMMVCRRVCKPIVDLTATMSRLASGDVSDDISGSDRDDEIGAMAAAVRVFKDN
                     MIKADRLAVEKDADNEVKMRRARVLDELTRAFETKVSELVGGLSSASSVMEETAQSMS
                     STASATNRQAGVVAAASEQTSANVQMVASATEELTSSVAEIGRQVAQSTEIAARAVES
                     ARRSGDTARSLAEGAQKIGDVVTLIQTIAAQTNLLALNATIEAARAGDAGRGFAVVAS
                     EVKSLAGQTAKATTEISGQIAAIQTASDETVTAIRNVVDVIAEIDQIGVAIASAIEQQ
                     GTATKEISKNVQQAARGTQEVTSNISGVQRAADDTGSAANQVLGAAEQLSSQSRDLAG
                     QVNRFLSEVRAA"
     gene            complement(4178..4723)
                     /locus_tag="VGA15_07010"
     CDS             complement(4178..4723)
                     /locus_tag="VGA15_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587483.1"
                     /translation="MSSTYGTAGQEARSEAASYTAGTPDRASMVRAGLIGGLAGGIII
                     WIYEALVWVGVQHLMPLAGIPRNATGLVFGKQVQEQLGTAAYFVGAGIHFLFALAWGV
                     LFAFLWPIFRRRGYEATLVALFYAVVIWIVMHAAIMVATTSHPDYFDPMVVIGGFMSH
                     FFYTVPLALIVKQRLAADATG"
     gene            4899..5489
                     /locus_tag="VGA15_07015"
     CDS             4899..5489
                     /locus_tag="VGA15_07015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX9587484.1"
                     /translation="MSNRILVFYGSYRSDRMGIRLAHYVVEGLRGRGDDVELIDAKAV
                     GLPMLDRMYKEYRKGEAPERLEQLAAQIRDADGFVFITGEYNWGIQPGLKNLTDHFLE
                     EWFWRPAAIASYSAGRIAGARAATAWHGTLSEMGMVVISSTIGVGPIAQTLSEKGEPI
                     GEGGKALAQTFARFADDLVWWIEAAKAQRARKSPPY"
     gene            5972..6157
                     /locus_tag="VGA15_07020"
     CDS             5972..6157
                     /locus_tag="VGA15_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587485.1"
                     /translation="MTSETQIHTIARQILERHGLGAIAQAARNALACESKGDAEEANE
                     WRHIEDAMKMMRGPHQS"
     gene            complement(6215..6649)
                     /locus_tag="VGA15_07025"
     CDS             complement(6215..6649)
                     /locus_tag="VGA15_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587486.1"
                     /translation="MRFRVRKFAHVLERLGLAMAGAASGLFVAALVGSSFALLTSQAF
                     LLFMMMGGAVGFYLGIDTPPLPFHPTNGPRSNGGTEAKIDSAEFLSAVGTFLATFSAF
                     ASVGVIVLRNDPHIAWTTAIMAGWVVGVVMQITAGAIARTRR"
     gene            6785..7720
                     /locus_tag="VGA15_07030"
     CDS             6785..7720
                     /locus_tag="VGA15_07030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX9587487.1"
                     /translation="MVFEDDLESSLDTVCKAAAGPREGVFGPASLTWRIDREAAIFLG
                     AGRALLLQLAHPWVAAGIAEHSRTLADPIGRFHRTFSTVFTMVFGTLDSALAAARRLH
                     RRHGAVTGILPSTAGRYAAGSAYRANEVSALRWVHATLVETALLTHDLVLPALTHGER
                     EQYWTEARLFAALFGIRQADLPSDWTSFTAYNEAMWRSDVLTVSPAARTIAEQILFGR
                     ARGLRVPKWYLALTAHLLPERLRAEFGLVFGEREARSMNLALARIRRLYPRLPMRLRT
                     VGPYQEALARLQGGKQPGLAVRSLNRLWIGRPMME"
     gene            8335..>8942
                     /locus_tag="VGA15_07035"
     CDS             8335..>8942
                     /locus_tag="VGA15_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9587488.1"
                     /translation="MLNRLTISALRKSVIAVMAAGVVAFLIATAWDSRGRLQTAARIS
                     VIADASSDAFKAMHNLRTDRATTSQNLNAEVIEADSVQALREYRAVINPAMRSLLDLA
                     ATVEFSDRNALLPALVQQFDALKALQTESMEAMGKPKASRRPALAREYTDAVTVLLQT
                     LEKLATQLAGAASHADPVIDQLLSIKQLAWLVRDTGGQAALLL"
BASE COUNT         1589 a         2818 c         2851 g         1684 t
ORIGIN      
        1 caggcacccg cggagctacg gcagcgtgac aaagggctcc gacctcctgg tggccgccct
       61 cgagaacgag ggcgtccaat gcatcttcgc cattcccggc gaggaaaacc tcgacgtgct
      121 ggagtcgctg cggcgctcga agatcaagct cgtgctgacg cggcacgagc aggccgctgg
      181 cttcatggcg gccacctatg ggcgtctcac cggacgcgcg ggcgtctgcc tgacgacgct
      241 cggtcccggt gcgctcaatc tgacgacggc tgcggcctac gcgcatctgg gcgcgatgcc
      301 gatggtcatg atcaccggac agaaggcgat ccggagcagc cgtcaggcgc gcttccagat
      361 cgtcgacatt gtcgcgacca tgcgacccct gaccaagatg gcaacgcaga tcgtctcggc
      421 gcagagtatt ccgaccctgg ttcgcgatgc tttccgggtc gcacagcagg agcggccggg
      481 cccggtgcat ctcgaactgc cggaggacat cgcggccgac aaggccgcgg ccgacatcgt
      541 tccccggcac gtggtcgaga ttccgatcgc gcctgcggct gcgatcgaac gcgccgccgc
      601 catgatcatg gccgccaagc ggccgctcgt catgctgggc gccgccgcga gccgcccgca
      661 cctcgccgga ccgttgtcgg cattcgtgcg acgggccaag attccgttct tcaacaccca
      721 gatggggaag ggcgccgtca atgcgggctc caatctctat atcggcacgg ccgcgctgtc
      781 ggaacgggat tgggttcacg aggccatcga ccaggccgac ctgatcgttt cgatcggcca
      841 cgataccatc gagaagccgc ctttcctgat gggccacgat ggaccgcagg tgatccatgt
      901 cggcgccacg ccggcaacgg tggagcaggt ctatttcccg caagccgaga tcgtcggcga
      961 tgtcggcgcc agcctcgcag gcctcgccga ccgcctggaa ggcaagctcc ccaatgcctc
     1021 ggcattgctg ggtttgcggg agggcatcct tgcacacctc gcggagggtt cgacagagga
     1081 tcggtttccg ttgacgccgc agcgcatcgt gcacgacgtc cgaaaggtga ttccgccgga
     1141 cggcatcgtt gccctcgaca acggcatgta caagatctgg ttcgcccgca actatcgcac
     1201 cagcgttgcc aacacgctgc tgctcgacaa cgcgctggcg acaatgggag cgggtcttcc
     1261 ctcggcgatc gcggcgtcgc tgatcaatcc ggacaagcgc gtgctcgcgg tctgcggcga
     1321 cggcggcttc atgatgaatt cccaggagct cgagacggcg gtgcggctca aacaaaacct
     1381 cgtcgtgctc gtgctcgagg accacgccta cgggatgatc cgatggaaac aggaagtcga
     1441 cgggttttcc gattttggtc tcgactttgg caatcctgat ttcgttgcct atgcgaagtc
     1501 ctatggcgcc aaaggctccc gcgtcggcaa cgccgccgaa ttcgcgacca tgctggaaac
     1561 cgctttccgg gaaggcggtg tgcatcttgt cgtggcgccg atcgactaca gcgaaaatat
     1621 gcgcgtcctc gtgcatgagc tgcattcggt atgattggcg actgatgccg gatctacgcg
     1681 gccctgacct ccgagaggaa gcggttgacc tgcccggcga gatccctcga ttgcgacgat
     1741 aattgctcgg cggcacctag cacctgattg gcggctgagc cggtgtcgtc ggccgcccgc
     1801 tgcacgcctg atatgttcga ggtgacttcc tgggtgccgc gcgcggcttg ctggacgttc
     1861 ttggaaattt ccttggtcgc ggtcccctgt tgctcgatgg cggaggcaat tgccacgccg
     1921 atctgatcga tctcggcgat cacgtcgacc acgttgcgga tcgccgtcac ggtctcgtcg
     1981 ctcgcggtct ggatcgccgc gatctgcccg gatatttcgg tggtggcctt cgcggtctgg
     2041 ccggcgagcg atttgacttc ggatgccacg acggcaaaac cccggccggc gtcgccggcg
     2101 cgcgccgcct cgatggtcgc gttgagcgcg agcaggttgg tttgcgcggc gatggtctgg
     2161 atcagggtca cgacgtcgcc gattttctgc gcgccctcgg ccagcgagcg cgcggtatcg
     2221 ccgctgcggc gggcgctctc gacggcgcgg gcggcgatct cggtggattg cgcgacctgg
     2281 cggccgatct ccgcgaccga ggatgtcaac tcctcggtgg cactggcgac catctgcacg
     2341 ttggcggagg tctgttcgga ggccgcggcg accactccgg cctgacggtt ggtggctgac
     2401 gcggtggaag acatcgattg ggcggtttct tccatcaccg aagacgccga cgacagtccg
     2461 ccgaccagtt cggagacctt ggtctcgaac gcgcgggtga gctcgtcgag cacgcgggcg
     2521 cgccgcatct tgacctcgtt gtccgcgtcc ttctccacgg ccaggcgatc cgctttgatc
     2581 atgttgtcct tgaagacccg gacggccgcc gccatcgcgc cgatctcgtc gtcgcggtcg
     2641 ctgcctgaaa tgtcgtcgga gacgtcgccg ctggcaaggc gcgacatcgt ggcggtgagg
     2701 tcgacgatcg gcttgcagac gcggcgacag accatcatca cgaggccgac gctcgcgctt
     2761 gcgaccgcgg cgaggccggc gagcgcgacc acgaagctga agcgcgcgtc ggaataggcc
     2821 gcgctgagga tgtgttcggc gttgtcgtag aaggcttcgc gcacctcgat aatcgcgcct
     2881 agaccgcgtt gcgactccgc gtagtaggtc tcgacatcgt gctcgtattt gccgctgacg
     2941 gcgccgtcct tcaccagctt ctgctcctgg ccgaaacgct cgacataggc ttcgttcatc
     3001 ttggtgagcg ccgccgccac gttggccggc gtcgcgggat tgccgcgcaa ttcctgcagc
     3061 gacaacagga tctggtcgtt cctgccctgg gagcggctca aatccagctt ttcggcatcg
     3121 gtggccacgc gcttggcggc gacgaggttt ttgtagaggc tggcgttggt cccgccgaca
     3181 tcgcgcaggg tccaggcgat gttggcgtaa ctcgcctgcc gataggcgcc gccgtcgagc
     3241 agcgccatct tgcggacctg ctcgtcgagc agcacggtga cggctgtatt gaacgccacg
     3301 ttgtcggcca cggccttctt ggcgccatcg cggcgggcct cgggcggacc gtcgagggcc
     3361 ttgtcgatgg tctcgcgcag tgctgcgaat tttacgttca gggcgtcgat gccgctggcc
     3421 acgccggcgc tatcctcgag cgtgccgggc agcgtcgcct tgatctgatt catcttttcg
     3481 cgggcgccgt cggtctgctt gcggtatttg tcgttgagct ccgcgcgcag ggccgggtcc
     3541 accgtggagg ggccgaacat gatgttggtg gcgaacccgc gctcggggtt catatagcgc
     3601 ggaatgtctc ccacggcccg gacgatctcc agccggcgct gcgcgacgga gatcttgtcc
     3661 atcgtctggt atttcgtggc ggcgacatag acggcaagac cgccgccgac agcggaaagc
     3721 gagacgattg cgaccgtcag cagggtgcca attttcatgg gttttgggcc attcacgagg
     3781 gtggagggag cggactttcg atcatcgtag ttactccgta ttaatcccga gtttacgctg
     3841 ccgcaggcga gggaggcagg tggctgcgtg gggggcacgg ggtcgcgcgg atgcacggcc
     3901 gctgacaggt tccgcaatcc gggaacgtaa cgtgacgcgc ctccatcaaa gcccgtcata
     3961 ttccgcgaaa tgtttagcct ggatagatca ctgacaggtg ttcggggaca tagctcacac
     4021 gatcgggagt atggattccg ggcctgcgcc aagtggcgca tccccgatgt gcaattgcac
     4081 atcggggatg acgacagtta tctggcacgc ttgctctcag ctaatcggcc catcgccgct
     4141 aatccgcagc gatgggcgtg gcgggggcga tgtcgtgtca gccggtcgca tccgctgcaa
     4201 gacgctgttt gacgatcaac gcgagcggca cagtgtaaaa gaaatgcgac atgaagcccc
     4261 cgatgacgac catcgggtca aagtagtcgg gatggctggt ggtggcgacc atgatcgcgg
     4321 catgcatcac gatccaaatg acgaccgcgt agaacagtgc caccagcgtc gcctcgtacc
     4381 cgcggcggcg gaagatgggc cacaggaatg cgaacagcac gccccatgcc agcgcgaaca
     4441 agaagtggat tcccgcgccg acgaaatacg ccgccgtccc aagctgctcc tgcacctgct
     4501 tgccgaacac caggcccgtc gcgttgcgtg gaatgcccgc caatggcatc agatgctgca
     4561 ccccaaccca gacaagggct tcgtaaatcc agatgatgat tccccccgcc agcccgccga
     4621 tcaaaccggc gcggaccatc gatgcccggt ctggcgtacc cgccgtgtat gacgccgctt
     4681 ccgatcgagc ttcctgtcca gccgtgccat aggtactact catgattgcg tttgctcccc
     4741 ttgatttctt gtttaagagt cggccgtctc gcatatgcga gatatgcaac ggatttcgcc
     4801 gttcctccga gacgggttat cttgcatccg gtaccgatct tcatcaagac aaatggcgct
     4861 tgctaatcca gcctgcgggc tgacgttgtg tggatgccat gagcaatcgt attctcgtgt
     4921 tctatggctc gtatcggtcc gaccgcatgg gcatccgcct tgcgcattat gtcgtcgaag
     4981 gcctgcgcgg ccgcggcgac gatgtcgaac tgatcgacgc caaagcggtc ggcctgccga
     5041 tgctggaccg gatgtacaag gaatatcgca agggcgaggc gcccgagcgg cttgaacaac
     5101 tggcggcgca aattcgcgac gccgacggtt ttgtcttcat caccggcgaa tataattggg
     5161 gcatccagcc cggcctgaag aatctcaccg atcattttct ggaagaatgg ttttggcgtc
     5221 ccgccgccat cgccagctat tcggccgggc gtattgccgg cgcgcgcgcc gcaaccgcct
     5281 ggcacggcac gctgtccgag atgggcatgg tggtgatttc cagcacgatc ggggtcggcc
     5341 cgatcgcgca gacactgtct gaaaagggcg agccgatcgg cgagggcggc aaggcgctgg
     5401 cgcagacgtt tgcgagattt gcggatgatc tcgtgtggtg gatcgaggcg gccaaggcgc
     5461 agcgggcgcg gaagtcgccg ccgtattgag ggctcggcta acgcgctcca cgctcgctca
     5521 tcgcggcagc agccgaacgc ggccggagcg gttttctgcc gcgatgggct tctcgggggc
     5581 tgtacgtgga ctttcagccc atttcaccgc ggggtcgtca caacttatgc attggaggtc
     5641 ggtgcggccg gagagggtaa caaccggtac caggcgtttt ccgcatctcg ggcagcgcgt
     5701 gatcggatcc atgagctcct cccgattttt tcttccataa cggggaaacg gaacttttgt
     5761 tccattcgag cgtttcgcgt tgaaccgtcc agccagcccg cgcgaggatc tccaagcgcg
     5821 cagcatctcg gctgcgcgtg gagatggtca tcaaattgtg cacggcagga aatggaacga
     5881 gacctccggc tcagcggttt caaggaagcc gcagcaggac cgcggaaccg gttcctgcgg
     5941 ctccgactgg cggaacatca gggagagcga gatgacgtcc gaaacgcaaa tccacacgat
     6001 cgcgcggcag atactcgagc gacatggctt gggagccatc gcccaggcag cccggaatgc
     6061 cctggcttgc gagagcaagg gcgacgccga ggaagccaat gagtggcgtc atatcgagga
     6121 cgccatgaag atgatgcgag gaccgcatca aagctgacgg attcaagtcc ccgttggctt
     6181 cgtccaggct tcgcttgcgg tcgggaacgg catctcaacg gcgggtgcgt gcaatcgctc
     6241 cggcagtgat ttgcatgacc acgccaacga cccatcccgc catgatcgct gtcgtccagg
     6301 cgatgtgcgg atcgttacgg agcacaatca cgccgaccga agcaaaagcc gagaacgtcg
     6361 cgagaaacgt acccaccgcg ctcaggaatt ccgcggaatc gatcttggct tccgtgccgc
     6421 cgtttgaacg cggaccgttt gttggatgaa aaggcagagg cggggtgtcg atgccgaggt
     6481 aaaatccgac ggcgcccccc atcatcatga agagcaggaa agcctgggaa gtcagcagcg
     6541 cgaagctcga ccccaccaga gcggcaacaa acaggccgct cgcagccccg gccatcgcaa
     6601 ggcccaggcg ttccagaacg tgggcaaatt tgcgcacgcg gaatcgcatc gtggtaaccc
     6661 cgcagacgac caagaggtta tgttaggacg tcttcctgtt gcgtgaaagc gattacctgc
     6721 agggcaggcg gccacccgcc gccattgcca gctattccgc gccttgcgaa ccggggaaat
     6781 gccgttggtc ttcgaagacg atcttgagag cagtctcgac accgtttgca aggcggcggc
     6841 cggaccacgt gagggcgtct tcggtccggc ttcactgacc tggcggatcg accgcgaggc
     6901 cgccatcttc cttggcgccg gccgcgcctt gctgcttcag ttggcgcacc cctgggtcgc
     6961 cgccgggatc gccgagcatt cccggacgct cgccgatccg attggccgtt tccaccgaac
     7021 gttcagcacg gttttcacga tggtgttcgg cacgctcgat tctgcgctgg ccgcggcccg
     7081 ccggctgcat cggcgtcacg gcgcggtgac gggaatattg ccgtcgacgg cgggccgata
     7141 cgcggcgggc tctgcctacc gcgccaatga ggtctcggcg ctgcggtggg ttcacgcgac
     7201 cttggtggag actgccttgt tgacccatga tctcgtcttg cctgcgctca cgcatggcga
     7261 gcgtgaacaa tactggaccg aggcccggct gttcgcggcc ttgttcggca ttcggcaagc
     7321 cgatttgcca tccgactgga cctcgttcac cgcctataac gaggcgatgt ggcggtcgga
     7381 cgttctcacc gtcagccccg ccgcccgcac catcgccgag caaatcctct ttggccgcgc
     7441 gagaggattg cgcgtgccga aatggtatct ggccctgacg gcccacttgc tgccggaacg
     7501 gttgcgcgca gaattcggat tggtcttcgg cgaacgcgaa gcgcgcagca tgaacctggc
     7561 gctggcgcgg atccgacggc tctatccccg acttcccatg cggctgcgaa ccgtgggtcc
     7621 ctatcaggaa gccctggcgc gcctgcaagg cgggaagcaa cccggcctgg ccgtgcgctc
     7681 gctcaacaga ctctggatcg gccgcccgat gatggaataa taatgcctcg gaaaacgggc
     7741 gccggctgca caacgagtcg tcatactccg cgaaagcgga gtatccagta cgccgcagct
     7801 ttccgattcc atcactgacg tctcggaata ctggatcgcc cgctttcgcg ggcctccgcg
     7861 aacttgacgc cagcgtcggg gcgtcaggac cacacgactt cgccgtccgc agcaatcgtc
     7921 tctcgtcagc gtgctccacg atcgctcaca agtctttcga cccgccctgc gatcccctcg
     7981 cgcgcaaaac gctgctgcgt ccaccgcatc caaccccgcg tccgtgacga tcgcgatacg
     8041 cccctccaga ggggtgggat gagtcgggtc taatggtgtt tctaccgggc aggcaaacag
     8101 aaaatttttg caaacaggac tggactgcta gcatcagatt gatcccgttc aacaaattac
     8161 cgaaaatacg caaagcgcgg aatagaccga cagcgctgcg cgagccgcgg cccgatggca
     8221 ggctccgcgc gagcccgtcc acctcggcat caggatctcg tagttcaccc agggtgcttt
     8281 gggccgaatt atttaccatt cgaccgtcaa ctcatcgaat cgttgggggt cctcatgctg
     8341 aatcgtctga ccatttccgc attgcgcaag tcggtcattg ccgtgatggc tgccggcgtg
     8401 gtggcttttc tgatcgcgac cgcctgggat tcccgggggc ggctgcagac cgccgcgcgc
     8461 atatcggtca tcgcggacgc ttcgtcggac gcattcaagg cgatgcacaa tcttcgcacc
     8521 gaccgtgcga ccacaagcca gaatttgaat gccgaagtca ttgaagccga ttccgtacag
     8581 gcgctccgcg agtatcgcgc ggtcatcaac ccggcaatgc gatcgctgct ggatctcgcc
     8641 gcgacggtgg aattttcgga ccgaaacgca ctgcttcccg cgctggtcca gcaatttgac
     8701 gcgctcaaag cgttgcagac cgaaagcatg gaggcgatgg gcaagccgaa ggcgtcccgt
     8761 cgccccgcgc ttgccaggga atataccgac gccgtgaccg tgttgctgca gacgctggaa
     8821 aagctggcca cgcagctcgc cggtgccgca agccacgccg accccgtgat cgatcaattg
     8881 ctctcgatca agcagctggc ctggctggtc cgcgataccg gcggtcaagc cgccttgttg
     8941 ct
//
LOCUS       DASWGM010000230         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_570012,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000230 DASWGM010000000
VERSION     DASWGM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570012"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /gene="nth"
                     /locus_tag="VGA15_07040"
     CDS             <1..361
                     /gene="nth"
                     /locus_tag="VGA15_07040"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX9587489.1"
                     /translation="LSEKLIAEFGGEVPRTRAEIESLPGAGRKTANVVLNMAFAEHTM
                     AVDTHVFRVANRTGLAPGDTPLKVELGLMHVIPPEFMLHAHHWLILHGRYTCLARKPR
                     CEVCLINDLCRWPEKTI"
     gene            476..1660
                     /locus_tag="VGA15_07045"
     CDS             476..1660
                     /locus_tag="VGA15_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX9587490.1"
                     /translation="MSQQDQIGPAPSARETGSSPSPPAAAAAATAPAPQSPPSASHPS
                     WGRLAIPLFAVLAAFGFIALATLRWDAWVGSEVIQTTNDAYVRAELTRLSSRVAGEVL
                     TVAANDFQRVKAGDLLVQIDPADYQAQVAQAEAGVIGAQAALDNLANQVELQYATIAQ
                     AEAQQVSALAQEVEARQEQERQQSLTQTEAGTRQKLEQATAGYARAQADVRASRALIA
                     AQRHQIEVLSGTKKQREADLQGAKAALAAARLRLGYTKIAAPFDGVVGERQVQAGDYV
                     NIGSNLINVVPLPDVYVMANYKETQLTRVKPGQPVEVTVDSFPSEKLTGRVERIAPAS
                     GSQFALLPPDNATGNFTKVVQRIPVRIAFDKGQPLLERLLPGMSVVTRIRTDEAATDG
                     GK"
     gene            1647..3275
                     /locus_tag="VGA15_07050"
     CDS             1647..3275
                     /locus_tag="VGA15_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587491.1"
                     /translation="MAENSDTGQGPLSRGGIAPQPLFAVGAVLLGSFLANFDSRLTSV
                     GLPDLRGAFSLGFDEGAWLSTAAIGSQIFIAPAVAWLATVFGLRRILGIPSLVYALVS
                     LVIPFVRDYNALIALSIVHGLLLGTFVPATLMIVFRNLPIRWWLPAIAIYSIRVGFSL
                     DTSTSLVGFYVDHWGWQWLYWQGVVIAPLMGLMVYLGTPHEPVNKELLRDADWGGMLL
                     LGTGVSMIYAGLDQGNRLDWLGSGTVMALLLGGGVLCVAFLINEMMVRQPWAHINVLF
                     SRNVGLSLIVILLYTLASLSNSSLVPNFLSVVGGLRPEQTGTLLVVYGALPMFVMVPV
                     SIFLLRHVDPRFVLVVGLSAFAAANLLGTQLTHDWALGDFIPIVLLQSIGQAFTLLPI
                     IILALSNADFSRATAFAAYIQIMRLGGAEIGVALMGTWLRVREQIHSNFIGQHLDSGS
                     AGVARMLKQLSDHFASHGAATAPARAVGSLSVLVARESNVLAYIDGFWLTFWIAILAL
                     GVVAFITRAPAGPFTPAPYGAVQAVMRRLRWSRT"
     gene            complement(3421..4173)
                     /locus_tag="VGA15_07055"
     CDS             complement(3421..4173)
                     /locus_tag="VGA15_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter family protein"
                     /protein_id="HEX9587492.1"
                     /translation="MLDAAVKALAQILSPPMRSILWRSIGLALVLIVVLAIGLQRLLG
                     WFATSGEVWAEAMLGPNFQTPLNVLAWILSIAAGLGVVFGAVFLMPAITSLVASVFVD
                     EAAEHVEREHYPAERPGTALPFGLAMTEGIKTALLAILVYLIALPFVFFAGAGFIAFF
                     IATAWLLGREYFELAAMRFRTPAEAKAMRKQNAATIFTAGLMIAAFVSIPIVNLATPL
                     FGMAFMVHMHKRLSGPRPELIEPGRKTSVPVA"
     gene            complement(4261..>4749)
                     /locus_tag="VGA15_07060"
     CDS             complement(4261..>4749)
                     /locus_tag="VGA15_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587493.1"
                     /translation="PTLLVEKGITVTKSLQYSFIVAFAYPIAPLLAASFADRLERKWI
                     ICGACIAIIGFGIAFSQFTAPALLILSGVMLTAANMTMSYAYHAYQTEVFPTSIRARS
                     SGLVYSMSRVSATFSGFIVAYVLREGGVSGVFGLITSAMVVVIVAIAAFGPNVRGKPL
                     DA"
BASE COUNT          823 a         1528 c         1534 g          864 t
ORIGIN      
        1 gctgtcggaa aaactgatcg ccgagttcgg cggtgaggtg ccgcgtaccc gcgctgaaat
       61 cgaatcgctg cccggcgccg gccgcaagac cgccaatgtc gtgctcaaca tggctttcgc
      121 agaacatacc atggcggtgg atacccacgt gtttcgcgtc gccaatcgca cggggctggc
      181 gccgggcgat acgccgctca aggtggagct tggtctgatg cacgtgattc cgcctgagtt
      241 catgctgcac gcgcatcact ggctgatcct gcacggccgc tacacgtgcc tggcgcgcaa
      301 gccccgctgc gaggtatgcc tgatcaacga tctctgccgc tggccggaaa agacgatctg
      361 aggcatggtc acgtcacgtc tcgccgcatg gtccggcatg cgggatcttc cctcagttca
      421 tcgtcgcgag agggagaatg atttttggga gaatgatttt gaggaggggg tctacttgag
      481 tcagcaggat cagatcggcc cggcgccatc cgcgcgtgag actggatcat cgccctcccc
      541 gccggcggct gctgcggcag ccacggcgcc agctccacaa tcgccgccgt ctgcttcgca
      601 tccttcgtgg ggccgccttg cgattccgct gttcgccgtg ctggccgcgt tcgggtttat
      661 cgcgctggcg acgctgcgct gggatgcctg ggtcggcagc gaagtgattc agaccaccaa
      721 cgacgcttat gtccgggccg agctgacgcg gttgagcagc cgggtcgccg gtgaagttct
      781 caccgtcgcc gcgaacgact ttcagcgcgt caaggccggc gatctcctgg ttcagatcga
      841 tcccgccgac taccaagccc aggtcgcgca ggcggaggcc ggcgtgatcg gcgcgcaggc
      901 cgcgctagac aatctcgcca accaggtcga gctgcaatac gccacgattg cgcaagccga
      961 ggcgcagcag gtgtctgcgc tcgcccagga ggtcgaagcc cgtcaggagc aggagcggca
     1021 acagtcactg acccagaccg aagccggcac aaggcaaaag cttgagcagg ccaccgcggg
     1081 ctatgcccgc gcccaggccg atgttcgggc gagccgggcc ctgatcgcgg cgcagcgcca
     1141 ccagatcgag gtgctctcgg gaaccaagaa gcagcgcgaa gcggatctgc agggcgccaa
     1201 ggccgcgctg gccgccgccc gcctcaggct cggttacacc aagatcgcgg cgccgttcga
     1261 cggtgtcgtt ggcgagcgcc aggtgcaggc cggcgactac gtcaatatcg ggagcaatct
     1321 catcaacgtc gtgccgctgc ccgacgtcta tgtgatggcc aactacaagg agacccaact
     1381 gacgcgggtg aagccggggc aacctgtcga ggtgaccgtc gacagtttcc caagcgagaa
     1441 actgacgggc cgcgtcgagc gaattgcgcc cgccagcgga tcgcaattcg cattgctccc
     1501 gcccgacaac gccaccggca atttcaccaa ggtggtgcag cgcatcccgg tccggatcgc
     1561 gttcgacaag ggccagccat tgctggagcg cctgctgccg gggatgtcgg tggtcacacg
     1621 aattcgcacc gacgaagcgg cgaccgatgg cggaaaatag cgacaccggc caagggccgc
     1681 tgtcgcgcgg cggcatcgcg ccccaaccgt tgttcgcggt cggcgcggtg ctgctcgggt
     1741 cttttctcgc caacttcgac agccgcctga cctcggtcgg attgccggac ctgcgcggcg
     1801 ccttttcgct cggcttcgac gagggcgcct ggctctcgac ggcggcgatc ggctcgcaga
     1861 tcttcatcgc gcccgcggtt gcctggctcg cgacggtgtt cggcctgcgg cgtattctcg
     1921 gcattccaag cctggtctat gccctggtct cgctggtgat cccgttcgtg cgcgactaca
     1981 acgcgctgat cgcgctcagc atcgttcacg gcctgttgct cggcaccttc gttcccgcga
     2041 ccctgatgat cgtctttcgc aacctgccga tccgctggtg gctgccggcg atcgcgatct
     2101 attcaatcag ggtcggcttc tcgctggata ccagcacgtc gctggtcggc ttttatgtcg
     2161 atcactgggg atggcagtgg ctgtactggc agggcgtggt catcgcgccg ctgatgggat
     2221 tgatggtcta cctcgggacg ccgcatgaac cggtcaacaa ggagttgctc agggacgccg
     2281 actggggcgg catgctgctg ctgggcaccg gggtctcgat gatttacgcc ggcctcgatc
     2341 agggcaaccg cctcgactgg ctgggatccg gcaccgtcat ggcgctgctg ttgggcggcg
     2401 gcgtgctgtg cgtggccttt ttgatcaacg agatgatggt ccgccagcca tgggctcata
     2461 tcaacgtgct gttttcgcgc aacgtcgggc tctcgctgat cgtgatcctt ctctacacgc
     2521 ttgccagcct gtcgaattct tcgctggtgc cgaattttct cagcgtcgtc ggcgggctgc
     2581 ggcccgaaca aaccggcacg ctgctggtgg tctatggcgc gctgccgatg ttcgtgatgg
     2641 tgccggtgtc gatctttctg ctcaggcatg tcgatccccg ctttgtgctg gtcgtcggcc
     2701 tgtcggcttt cgccgcggcg aacctgctcg gaacccaact gacgcacgac tgggcgctcg
     2761 gcgatttcat cccgatcgtc cttctgcaat cgatcgggca ggcgttcacg ctgctgccga
     2821 tcatcatcct ggctttgtcc aatgccgact tttcccgggc gacggcgttc gcggcctata
     2881 tccagatcat gcggctcggt ggggcggaaa tcggcgtggc gctgatgggc acatggttgc
     2941 gcgtccgcga gcagatccac tccaatttca ttggacagca tctcgatagc ggtagcgccg
     3001 gcgtcgcccg gatgctcaag cagctttcgg atcattttgc gagccacggg gcggcgaccg
     3061 cgccggcgcg cgccgtcggg tcactctcgg tgctggtggc gcgcgagtcg aacgtgctgg
     3121 cctatatcga cggcttttgg ctgacgttct ggattgcgat tttggccctc ggcgttgtgg
     3181 ctttcatcac ccgcgcgccg gcgggacctt ttacgcccgc gccgtacggc gccgtgcagg
     3241 ccgtgatgcg gcgattgaga tggtctcgga cttaaggggc gcaggtgtcc cggacgcggt
     3301 gcagcgttct tcacggtgca ccgcagagcc gggacccacc tcgccgcata aacgatgggc
     3361 cccggatcag cagcgcaccg caagcgcgct gcgcagcatc cggggcacgc ttggctctcg
     3421 ctaagccacc ggcacgctcg tctttcggcc cggctcgatc aattccggcc ttggcccact
     3481 caatcgcttg tgcatgtgaa ccatgaacgc catgccgaac agcggcgtcg ccaggttgac
     3541 gatcgggatc gacacgaacg ccgcgatcat cagccccgcg gtgaagatgg tcgcggcgtt
     3601 ctgcttgcgc atcgccttgg cttccgcggg ggtgcgaaac cgcatcgccg cgagttcgaa
     3661 atattcccgc cccaagagcc atgccgtcgc aataaagaat gcgatgaagc cggcgccggc
     3721 gaagaacacg aagggcagcg cgatcagata gaccaggatc gcgagcaagg cggtcttgat
     3781 gccttcggtc atggcgaggc cgaacggcag cgcggtgccg ggacgctccg ccggatagtg
     3841 ttcgcgctcg acatgctcgg cggcttcgtc gacgaacacg ctcgccacca gcgaggtgat
     3901 cgcgggcatc aagaacaccg cgccgaacac gacgcccagc ccagcagcga tcgagaggat
     3961 ccaggcgagc acgttgagcg gggtttgaaa gtttggaccg agcatcgcct cggcccagac
     4021 ttcgcctgag gtcgcaaacc agccgagcag ccgctgcagg ccgatcgcca gcaccacgat
     4081 cagcaccaat gccagcccaa tcgatcgcca caggatggaa cgcatcggcg gcgacagaat
     4141 ttgagcaagc gccttgacgg cggcgtccaa catgatggtc ctatctttcg cgaaagcccc
     4201 cgcgagaggt agccatgacc ccggcgctcg gcaagagcca agttcaatca acaagttccg
     4261 tcaagcgtcg agcggtttgc cgcgcacgtt cggaccgaag gcggcgatcg cgacgatcac
     4321 gaccaccatc gccgacgtga tcaggccgaa cacaccgctg acaccgccct cgcgcagcac
     4381 ataggcgacg atgaagcccg agaacgtcgc gctgacccgg ctcatcgagt agaccagccc
     4441 cgaggaccgc gcccggatcg aggtcggaaa cacttcggtc tggtaggcgt gataggcgta
     4501 ggacatggtc atgttcgcag ccgtgagcat cacgccgctc aggatcaaca gcgccggcgc
     4561 cgtgaactgc gaaaacgcta ttccgaatcc gatgatcgcg atgcatgcgc cgcagatgat
     4621 ccatttgcgc tcgagccggt cggcaaagct cgcggccagc agcggcgcaa tcgggtaggc
     4681 gaacgcgacg atgaaggaat attgcaggct cttggtgacg gtgatgccct tttcgaccag
     4741 cagcgtcgg
//
LOCUS       DASWGM010000231         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_433471,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000231 DASWGM010000000
VERSION     DASWGM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433471"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1589)
                     /locus_tag="VGA15_07065"
                     /pseudo
     CDS             complement(120..1589)
                     /locus_tag="VGA15_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240773.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
     gene            1864..3228
                     /locus_tag="VGA15_07070"
     CDS             1864..3228
                     /locus_tag="VGA15_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX9587494.1"
                     /translation="MDGGSSEGSGARFGGYVEALGVVLGHADRQQPMHDYCLGLLMPI
                     ERKSVEPMAAVTAPAQVAAKHQSLLHFVGNAPWSDAAMLGKVAQLVLPAVERSGPIEA
                     WIIDDTGFPKKGRHSVGVTRQYCGQLGKQDNCQVAVTLSLANHDASLPVAYRLYLPED
                     WAKDQARRDRARVPETIAFQTKPEIAFEQIKAARGAGLPQGVVLMDAGYGNDTGLRTD
                     ISSLGLAYVAGIGPNTSVWPPGAAPLPPQPGTGRGRPPKLLRRDEQHQPISVKALALS
                     LPAEAWQPIRWREGSADWLASRFARLRVRPAHRDNLLAEPRAEEWLLIEWPEDEAEPT
                     KYWFATLPEDIAFDRLVDLAKLRWRIERDYQELKQELGLGDYEGRGWRGFHHHATLCI
                     AAYGFLIAERGAIPPSGPRVSPQLSGSAIPNGYRPRGAADPTRTPHPKLDRDHATTPH
                     RRSR"
     gene            3421..>3625
                     /locus_tag="VGA15_07075"
     CDS             3421..>3625
                     /locus_tag="VGA15_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587495.1"
                     /translation="MAVFSKSRLFPQPANKKLIAELRDQLNKAQETILQLQQLLQAQS
                     ENLHEGIKLTPNQRTVVDMLLVTN"
BASE COUNT          703 a         1112 c         1121 g          689 t
ORIGIN      
        1 tcggctacaa gagatgttta agaacccgtt gatccgaaaa agctggatcg aaggcgcacc
       61 ctctcaacga cttgccagag aggcggaatg gaggacggcc tcctccagcc gtactaatct
      121 tactgcgtca gtaatcggga attcgtgccg tcttgcggat cggcgcgttg caacaagggc
      181 atctcgggag agttctggca agggcggcga taaggcgccg ccgcatcgtg gcgatcgagt
      241 tcgggatgtg gcgttcgggt ctgatcggtg gcgcctctgg gtcggtaacc ctcgggaatg
      301 gcaggtcgcg agagctgcgg ggaggaacgt ggtcctgagg gggggatggc gcctcgttcg
      361 gcgatcaaga agccgtaggc ggcgatgcag agtgtggcgt gatggtggaa gccgcgccat
      421 ccgcgccctt cataatcacc aaggccgagt tcctgtttga gctcctgata atcacgctcg
      481 atgcgccacc gcaactttgt cagatcaacc aagcggtcga aggcgacatc ctcggacaag
      541 gtggagaacc aatacttggt cggttcggtt tcgtcctgcg gctgctcgat cagaagccac
      601 tcctcggcgc gcggctcgct cagcaagttg tcacgatgcg ccggccgcat tctccgccgg
      661 gcaaagcgcg acgagagcca atccgcggtt ccctcgcgcc aggtgatggt ttgccacgcc
      721 tcttccggca ggctcagggc aagtgccttg acggtgattg gctggtgctc cccgtcgcgt
      781 tgaagccgcg tctgtggtcg tccgcgccac gatcgggtct gcggcggcaa aggggcggcg
      841 cccggtggcc agaccgaggt attgggaccg atcccggcca catagctcat gccgagggcg
      901 gtgatctccg ttcgcaggct ggtatcgtta ccgtaaccgg catccatcag caccacaccc
      961 tgaggcaacc cggccgttcg ggccgctttg atctgctcaa gggcgatctg cggcttggtt
     1021 tggaaagcga tcgcctccgg cactttggcc ttgtgccgac gcgcctgatc cttggcccaa
     1081 tcctcaggga gatagagccg ataagcgatc ggtaggctgg catcatggtt ggccagcgac
     1141 agcgagaccg cgacttggca gttgtcctgc ttgccgagct ggccgcaata ttgtcgggct
     1201 accccgaccg agtgccgccc cttcttggga aagcctgtgt cgtcgatgat ccaggcctcg
     1261 accggcccgc tgccctcgac cgccggcagc accagttcgc ctaccttggc cagcatcgcg
     1321 gcgtccgacc acggcgcgtt gccaacaaaa tgaagcaacg attgatgctt cgcggcgact
     1381 tgccccacgg gtcaagcccg tgggctgaag gcccacggtg ctgtcaccgc cgccatcggt
     1441 tcgacgctct tgcgctcaat cggcattaac agccccaggc agtaatcatg catcggctgc
     1501 tgtcgatcag catggcccag caccacccca agcgcctcga catagcccgc aaagcgcgct
     1561 tcactcccct cactcgatcc gtcgtccatt ccggtccccc gatccggatg aagatagacc
     1621 taaatactac atcttgcggt cgtcagctat ataaatacat gatctcgaat tttgtgactc
     1681 agtaagatta gacgctgatt acactcgttc gtgggcgcac cgtcgcgatc gccgaggagg
     1741 tcgccgatca gaacgcgctc ctaatcttac tgagtcacaa aattcgagat cgtgtattca
     1801 tatggatagt aaccgcaaga tgtagtattt tggcccatct tcatccggga tcggggggcc
     1861 gggatggacg gcggatcgag cgaggggagt ggagcgcgat ttgggggcta tgtcgaggcg
     1921 ctcggcgtgg tgctgggcca tgccgatcga cagcagccga tgcatgatta ttgcctgggg
     1981 ctgttgatgc cgattgagcg caagagtgtc gaaccgatgg cggcggtgac agcaccggcg
     2041 caggtcgccg caaagcatca atcgttgctg cattttgttg gcaacgcgcc gtggtcggac
     2101 gccgcgatgt tgggcaaggt ggcacagctg gtgctgccgg cggttgagcg cagcgggccg
     2161 atcgaggcct ggatcatcga cgacacaggc tttcccaaga aggggcggca ctcggtcggg
     2221 gtgacccggc aatattgcgg ccagctcggc aagcaggata actgccaggt cgcggtgacg
     2281 ctgtcgctgg ccaatcatga tgccagccta cccgtcgctt atcggctcta tctccctgag
     2341 gattgggcca aggaccaggc gcggcgggac agggccaggg tgccggaaac gattgctttc
     2401 cagaccaagc ccgagatcgc ctttgaacag atcaaagcgg cccgcggggc agggctgccc
     2461 caaggggtgg tgctgatgga tgccggctac ggcaacgaca ctgggctgcg gaccgatatc
     2521 agctctctcg gcctggccta cgtggcgggc atcggtccca acacctcggt ctggccgccg
     2581 ggcgcggccc ccttgccgcc gcagcctgga acggggcgcg gacgaccgcc caagctgctg
     2641 cgacgcgatg agcagcacca gccgatttcg gtcaaggcgc tcgctctcag cctgccggca
     2701 gaggcgtggc aacccatcag gtggcgcgaa ggcagcgcgg actggctcgc ttcacgcttc
     2761 gcacggctgc gggtgcggcc ggcgcaccgc gacaacttgc tggccgaacc gcgcgccgaa
     2821 gaatggctgc taatcgagtg gccggaagac gaagccgagc cgaccaagta ttggtttgcc
     2881 accttgcccg aggatatcgc cttcgatcgc ctggtcgacc tggcaaaatt gcggtggcgg
     2941 atcgagcgcg attatcagga gctcaaacag gaactcggcc ttggtgatta tgaagggcgc
     3001 gggtggcgcg gcttccacca tcacgccaca ctctgcatcg ccgcctacgg cttcttgatc
     3061 gccgaacgag gcgccatccc cccctcagga ccacgtgtct ccccgcagct ctcgggatct
     3121 gccattccca acggttaccg acccagaggt gccgccgatc cgacccgaac gccacatccc
     3181 aaactcgatc gcgaccatgc gacgacgcct catcgtcgct ctcgctagga ccctcccgag
     3241 atgcccttgt tgcaacgcgc ctatccgcaa gacgagacga attcctgatt actgacgcag
     3301 taagattaac aggctgcgga aaaagtcgat tgatcgtcgt ttcgctgcca ctcatcgact
     3361 ggggcacgtc cgtgacccat tgatctaccg tgaccgtaat tccggcatcg agtccatttc
     3421 atggccgttt tttctaaatc gcgacttttc ccgcagcctg ctaataaaaa gctaattgca
     3481 gagctacgag accagcttaa caaagcgcaa gaaacgattc ttcagcttca gcagcttttg
     3541 caagcacaaa gcgaaaatct ccatgagggc atcaagctga cgccgaacca gcggacggtc
     3601 gtcgatatgc ttctggtgac gaacg
//
LOCUS       DASWGM010000232         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_851501,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000232 DASWGM010000000
VERSION     DASWGM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851501"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VGA15_07080"
     CDS             <1..636
                     /locus_tag="VGA15_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9587496.1"
                     /translation="PTMKERILETADRLFYRQGIRAVGVDTVAAEIGISKRTLYNHFP
                     SKNQLISAYLERRFRPVPTSDKPPAEQILRTFDRLERGFADKGFRGCPFVNAVAEMGA
                     EDHAVRKIAIAFKDSRRAWFRDLLLQLDVANPDELATQLQLLVDGAIANDLVRNDPSV
                     ARAAKDAARVLLRNAGVRVGPPDQVHKPGRVAKKRSSSPRRRGPIRRDGAV"
     gene            complement(722..2095)
                     /locus_tag="VGA15_07085"
     CDS             complement(722..2095)
                     /locus_tag="VGA15_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HEX9587497.1"
                     /translation="MSARILVVDDVPANVKLLEARLSAEYFDVLTASNGAEALEICAR
                     AECDIILLDVMMPDIDGFEVCRRLKSNPATHFIPVVMVTALDSPADRVRGLEAGADDF
                     LTKPVSDIVLIARVRSLTRLKMMTDELRMRAITSLEIGVQAPERSAVADTGKGGRILL
                     VDDRPSSYEKLAPVLSSEHTVDVEINPAEALFHAAEGNYDLLIVSLGLDNFDGLRLCS
                     QARSLERTRHVPILAIADADNNMRLLRGLEIGVNDYLLRPVDKNELLARARTQIRKRR
                     YTDHLRDNVQNSIEMAITDALTGLHNRRYMESHLGTLAEQAATRGKPLALMMLDIDFF
                     KSINDNYGHDAGDDVLREFAVRVRKSIRGIDLACRYGGEEFVIVMPETDLHVAGMVAE
                     RLRRSIAGEPFAVNKGTKRIEVTISIGLSTLELKGEPVGDVLKRADTALYKAKNDGRN
                     RVVAAAA"
     gene            complement(2107..2472)
                     /locus_tag="VGA15_07090"
     CDS             complement(2107..2472)
                     /locus_tag="VGA15_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9587498.1"
                     /translation="MAKTVLIVEDNELNMKLFRDLLEAHGYQTSGTSNGFEALDLVRS
                     LRPDLILMDIQLPQVSGLEVTRWIKDDPELRSIPVVAVTAFAMKGDEERIREGGCEAY
                     LSKPISVGKFIETVRRFIG"
BASE COUNT          472 a          800 c          760 g          468 t
ORIGIN      
        1 ccgaccatga aggagcggat cctcgaaacc gcggaccggc tgttttatcg gcagggcatc
       61 cgcgccgtcg gcgtcgatac cgtcgcggcc gaaatcggca tctccaagcg cacgctctac
      121 aaccactttc cctcgaaaaa ccagttgata tcagcctatc tcgagcgccg gttcaggccg
      181 gttccgacct ccgacaagcc gccggcagaa caaatcctgc gcaccttcga ccggctggag
      241 cgcggcttcg ccgacaaggg ctttcgcggc tgcccgttcg tcaacgcggt tgccgaaatg
      301 ggcgctgagg atcatgccgt cagaaagatc gcgatcgcct tcaaggacag ccgccgcgcc
      361 tggttccgcg acctgttgct gcagctcgat gtcgccaatc cggatgagct tgcgacgcag
      421 ctccagcttc tcgtcgatgg cgcgatcgcc aacgatctgg tgcggaacga tccgtcggtg
      481 gcgcgcgccg caaaagacgc cgcgcgcgtg ttgctcagga atgcgggtgt cagggttggc
      541 ccgcccgatc aggtacacaa acctggacgc gtcgcgaaaa aacgatcgtc gtccccgcgc
      601 aggcggggac ccatacgccg tgacggtgca gtgtagaaca ggcggctgtt acaagatctc
      661 ttttaaacca tgaccgctgg cggttatggg tcccggcgtt cgccgggacg acgtcgccgg
      721 cttacgccgc cgccgccacc acgcggttgc ggccgtcgtt ttttgctttg tagagcgcgg
      781 tgtcggcgcg cttgaggacg tcgccgacgg gctcgccctt gagctcgagg gtcgacagcc
      841 cgatcgagat cgtgacctcg atgcgctttg tccctttgtt gaccgcgaac ggctcacccg
      901 cgatcgaacg ccgcagccgt tccgccacca tgcccgcgac atgcagatcg gtttccggca
      961 tcacgatgac gaattcctcg ccgccgtagc ggcaggccag atcgatgccg cggatcgact
     1021 tgcgcacccg gactgcgaat tcgcgcaata cgtcgtcgcc ggcgtcgtgg ccgtaattgt
     1081 cgttgatcga tttgaaaaaa tcgatgtcga gcatcatcag cgccaagggc ttgccgcggg
     1141 tcgcggcctg ctcggccagc gttcccagat ggctttccat gtaacggcgg ttatgcaggc
     1201 cagtcagggc gtccgtgatc gccatctcga tcgaattctg cacattgtcg cgcagatggt
     1261 cggtgtagcg ccgcttgcgg atctgggtgc gcgcacgcgc caacagttcg ttcttgtcga
     1321 ccgggcgcaa aaggtaatca ttgacgccga tctccaggcc gcggagcagc cgcatattgt
     1381 tgtcggcgtc ggcgatggcg agaatgggca cgtgccgggt tcgctccagc gaccgcgcct
     1441 ggctgcacag ccgcaagcca tcgaaattat caaggccgag cgagacgatc agaagatcgt
     1501 aattgccctc ggcggcgtga aacagcgctt ccgccggatt gatttcgacg tcgacggtat
     1561 gctcgctgct cagcaccggc gccagcttct cgtaggatga cggccggtcg tcgaccaaga
     1621 ggatgcgtcc gcccttcccg gtgtcggcca ccgcacttcg ctcgggcgcc tgcacgccga
     1681 tctcaagcga ggtgatggcg cgcatgcgca gttcatcggt catcatcttc agccgggtca
     1741 gcgaacgcac ccgtgcaatc agcacgatgt ccgataccgg cttggtcagg aaatcgtcgg
     1801 cgccggcttc aaggccgcgc acgcgatcgg ccgggctatc caacgccgtc accatcacca
     1861 ccggaatgaa atgcgttgcc ggattggact tcaaccggcg gcaaacctcg aaaccatcga
     1921 tatcgggcat catcacgtcg agcaggatga tgtcgcattc ggcgcgcgcg cagatctcga
     1981 gcgcctcggc gccgttggac gcggtcagga cgtcgaaata ttcggccgac agccgcgcct
     2041 ccaacagctt gacgttggcg gggacgtcgt cgacgaccag aatgcgcgcg gacatcgaag
     2101 ctctccctag ccgataaacc gtcggacggt ttcaataaat ttgccgaccg agatcggttt
     2161 tgaaagatag gcctcgcagc cgccctcgcg gatgcgttcc tcgtcgccct tcatcgcgaa
     2221 ggccgtgacc gcaaccaccg gaatcgagcg caactcggga tcatccttga tccatcgggt
     2281 tacctccagt ccggaaactt gcggcagctg aatatccatg aggatgagat cgggacgaag
     2341 cgagcggacg agatcaagcg cctcgaaacc gttgctggtg ccggaggtct gatagccatg
     2401 cgcttccaac agatcgcgaa agagcttcat attgagctcg ttgtcctcca cgatcaggac
     2461 ggtttttgcc atccgtccct ccaatcccca ggcgacaggc
//
LOCUS       DASWGM010000233         7537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1820727,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000233 DASWGM010000000
VERSION     DASWGM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820727"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VGA15_07095"
     CDS             <1..510
                     /locus_tag="VGA15_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX9587499.1"
                     /translation="ARPVFLVDWRQRNSTFFNALQVERNVMFLILTMIVLVAALNIVS
                     GLIMLVKDKGSDIAILRTMGASQGSIMRVFLITGASIGVVGTLTGLFVGMLICLNIES
                     IRQFLSWLTSTELFSPELYFLSKLPAEIDFGETSAVVIMALTLSFLATLYPSWRAARL
                     DPVDALRYE"
     gene            518..1216
                     /locus_tag="VGA15_07100"
     CDS             518..1216
                     /locus_tag="VGA15_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587500.1"
                     /translation="MEQGEEDVPVVYLHEIKRQYTQGETTLTILNGAKLALWAGQSVA
                     LVAPSGAGKSTLLHIAGLLETPDDGEVYIGGTPTSGLSDIERTQIRRTDIGFVYQSHR
                     LLPEFSALENVMLPQMISGLRRSETVKRATEILTYLGLGDRIKHRPAELSGGEQQRVA
                     IARAVANAPRVLFADEPTGNLDPHTADHVFQALTQLVKATRVAMLIVTHNMELAGRMD
                     RRVSLVDGQVVELT"
     gene            complement(1281..2360)
                     /locus_tag="VGA15_07105"
     CDS             complement(1281..2360)
                     /locus_tag="VGA15_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9587501.1"
                     /translation="MKAAILKTWGSPLAIESLPDPVLGTGEIIVDVVATRVLSYANEV
                     FSGERKYLLELPVVPGPGAIARVRAVGPDATHVAAGDWVYCDPTVRSRDDALNPDITL
                     QGWSARGEGGLRLQKHFRHGSWAEQMLVPTENVKPIGPIAEADAVQWCALGTLLVPYG
                     GFLAAKLQPGETVLISGATGNFGSAAVAVALAMGAACVIAPGRNEAVLEDLARRFGDR
                     LRPVKLSGNEDDDSERMRRAAPGPIDCVLDILPPSADTTTVRAAAMTVRPYGRVALMG
                     GVGMSGGAGLELPYPWIMRNCITIHGVWMYPPEATLGLVNLIKSGLLRLDHFKATTFD
                     LDHANEAVAHAAANSGPFKMTVILP"
     gene            2475..3248
                     /locus_tag="VGA15_07110"
     CDS             2475..3248
                     /locus_tag="VGA15_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9587502.1"
                     /translation="MADPRRSEFGDFLRSRREKLSPKAVGLPTGRRRRTAGLRREEVA
                     ELAGIGVDWYIRLEQGRTVSPSVTTIDALARALRLGKAEHAHLRALASDADRRAFSRE
                     NVPDAIRRLVDNLNQPAYITGRRWDVLAWNAAAEEIFAFSRLAEEDRNTLISVLTNPH
                     TRGLFGATWADEAKRMVAQFRATHDLWAGDPAFLSLLERLRQGCPEFAIWWEAHDIGS
                     VTAGRKLLSHPKKGSLRFEYATFQANDDPALKLVIYTPV"
     gene            complement(3263..3516)
                     /locus_tag="VGA15_07115"
                     /pseudo
     CDS             complement(3263..3516)
                     /locus_tag="VGA15_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135388.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
     gene            complement(3516..3623)
                     /locus_tag="VGA15_07120"
                     /pseudo
     CDS             complement(3516..3623)
                     /locus_tag="VGA15_07120"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3883..4161)
                     /locus_tag="VGA15_07125"
     CDS             complement(3883..4161)
                     /locus_tag="VGA15_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HEX9587503.1"
                     /translation="MRNLVLALLAASGLAMIGAAPAAAVGTRYPFCIQGNEYPGLSNC
                     TFTSYEQCQATASGRFLYCVENPYYNPGEPDPRAYRSRHSVRSVYPSY"
     gene            complement(4365..4532)
                     /locus_tag="VGA15_07130"
     CDS             complement(4365..4532)
                     /locus_tag="VGA15_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEX9587504.1"
                     /translation="MKLSEVIRRAIKLGEGNGTLTFDQLNELIPSNLEPEDIEAVLLS
                     LSAKDIQIVES"
     gene            5072..>7537
                     /gene="dnaE"
                     /locus_tag="VGA15_07135"
     CDS             5072..>7537
                     /gene="dnaE"
                     /locus_tag="VGA15_07135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9587505.1"
                     /translation="MSSAGFVHLHVHSAYSLLKGSIKIQRLGELAKADRQPALALTDT
                     DNMFGALEFSDKMAGYGIQPIIGCELAVDFGDQDPSARNALAAGPARIVLLAARERGY
                     RSLMRLNSRAFLETPVHQSPHIKFEWLQGDAEDLIALTGGPEGPIALAINADQAALAA
                     TRCDRLVSLFGDRLYIELQRHGIEKERRAEAGLIDLAYAKGLPLVATNEPYFATADDY
                     EAHDALLCIAGGRLVAETDRDQLTPDHRFKTRAEMAVLFADVPEALASTIEIAERCSF
                     RPVTRKPILPRFTVGAGTNAAEAESDEAAELRRQAEEGLSNRLRVHGLSQGSSEEDYR
                     ARLAFEIDVITRMNYAGYFLIVADFIQWAKAEGIPVGPGRGSGAGSLVAWALTITDLD
                     PIRFGLLFERFLNPERVSMPDFDIDFCQDRRGEVIDYVQQRYGRDQVAQIITFGTLQA
                     RGVLRDVGRVLQMPYGQVDKLTKLVPQNPAAPVTLSAAIASEPKLQAFRDEDPVVARA
                     FDIAQRLEGLTRHASTHAAGIVIADRPLSELVPLYRDPKSDMPVTQFNMKWVEPAGLV
                     KFDFLGLKTLTVLDVAVKLLRRRGVDVDLATLPLDDATSYQMLARGDVVGVFQVESQG
                     MRRALVDMRPDRFEDIIALVALYRPGPMANIPTYCARKHGDEEPEYLHPMLEPILKET
                     FGVIIYQEQVMQIAQLMAGYSLGEADLLRRAMGKKIRSEMEKQRAGFVAGAQRNNVPK
                     GQADTIFELLAKFADYGFNKSHAAAYALVSYHTAYMKAHYPVEFLAASMTLDLSNTDK
                     LSEFRAEAQRLGIKVEAPSVNRSG"
BASE COUNT         1400 a         2357 c         2380 g         1400 t
ORIGIN      
        1 gcacggccgg tgttcctggt ggactggcgg cagcgcaact cgaccttctt caatgcgctt
       61 caggtcgagc gcaacgtgat gttcctgatc ctcaccatga tcgtgctggt cgcggcgctc
      121 aatatcgtct cgggcctgat catgctggtg aaggataagg gcagcgatat tgcgattctg
      181 cgcaccatgg gcgcttcgca gggatcgatc atgcgggtgt tcctgatcac gggtgcctct
      241 atcggtgtgg tcggcacctt gaccgggctt ttcgtcggca tgctgatctg cctgaatatc
      301 gaatcgatcc gacagtttct gtcctggctc accagcaccg aattgttttc tccagagctc
      361 tattttctct ccaagctgcc ggccgaaatc gatttcggcg agaccagcgc ggtcgtgatc
      421 atggcgctga cgctatcctt cctcgcgacg ctgtatccat catggcgtgc cgcccgcctc
      481 gatcccgtcg acgcgctccg gtacgagtga gggcgagatg gagcaggggg aggaagatgt
      541 accggtcgtc tatctccacg agataaaacg tcagtacaca caaggggaaa cgacgctgac
      601 cattctcaac ggcgccaagc ttgcgctgtg ggcgggacag tcggtcgcgc tggtggcgcc
      661 gtcgggcgcc ggcaagtcga cgctgctgca tatcgcaggc ctgttagaga cccccgatga
      721 tggcgaggtg tatatcggcg gcacgcctac ttccggatta tccgatatcg agcgaaccca
      781 gattcgccgc accgacatcg gctttgtcta tcagtcgcac cggctgctgc cggaattctc
      841 cgcgctcgaa aatgtcatgc tgccgcagat gatcagtggc ctcaggcgct ccgagactgt
      901 caaacgcgcc accgaaatcc tcacctatct tggcctcggc gatcgtatca agcaccggcc
      961 ggcggaactg tcgggcggcg agcagcagcg ggtcgcgatc gcgcgtgcgg tcgccaacgc
     1021 gccgcgcgtg ctgtttgcgg acgagccgac cggaaatctc gacccccaca ccgcggacca
     1081 tgtgttccag gccctgacgc aactggtcaa ggcgacccgg gtggcgatgc tgatcgtaac
     1141 ccacaacatg gagctggccg gccggatgga ccgccgcgtg tcgctggtgg acggccaggt
     1201 cgtcgagctg acgtagggtt tgcgtcagcg gccggatcag actctggcga tcggaaactc
     1261 cctgcgaaaa gcgaagcccg ttacggcagg atcacggtca tcttgaaggg accgctattg
     1321 gcggcggcat gggcgacggc ttcgttggca tggtccagat cgaacgtcgt ggccttgaaa
     1381 tgatcgagcc tcagcagtcc tgacttgatg aggttgacca gaccgagggt ggcctcgggc
     1441 gggtacatcc agacgccgtg gatggtgatg cagtttcgca tgatccatgg ataaggcaat
     1501 tccagcccgg cgcctcccga catcccgacg cctcccatca gcgccactcg gccatagggc
     1561 cgcaccgtca tggcggcggc gcgcaccgtt gtcgtgtcgg ccgatggtgg caggatatcc
     1621 agcacgcagt cgatcgggcc cggtgcggct ctccgcatcc gctcagaatc gtcgtcctcg
     1681 ttgccgctga gttttaccgg ccgaagccgg tcaccgaatc gacgcgccag atcctcgagc
     1741 accgcttcgt ttcgtcccgg tgcgatgacg caagccgcgc ccatcgcgag ggcgaccgcg
     1801 acggcggcgc tgccgaaatt gccggtggcg ccgctgatca aaaccgtttc tcccggctgc
     1861 agcttggcgg caagaaaacc gccatagggc acgagcagcg ttcccagcgc gcaccattgc
     1921 accgcatccg cctcagcaat cggtccgatc ggcttgacgt tctcggtcgg aaccagcatc
     1981 tgctcggccc atgagccatg gcggaagtgt ttttgcaggc gcaggccgcc ttcgccgcgg
     2041 gcgctccagc cctgcagggt gatgtcggga ttcagcgcat cgtcgcgcga gcgcacggtc
     2101 gggtcgcaat agacccaatc gccggcggca acatgggtcg cgtcggggcc gaccgcgcgc
     2161 acccgggcga tggcgccggg tcccggcacg accggcagct cgagcaggta tttccgctcg
     2221 ccgctgaaga cttcgttggc ataggacaga acgcgggttg ccacgacatc gacgatgatc
     2281 tctcccgtgc ccaggaccgg atcgggcaag gattcgattg caagtggcga tccccaggtc
     2341 ttcaagattg cagctttcat ggtcggcctt ctctgttgtc tcggatgaac cagcatcgca
     2401 tgctgacttt cgcacatgca ggcctggtat tatcctagga ctgaagagtg cctgtcaggc
     2461 gagaggatga agtcatggcg gatccgcggc gaagtgaatt cggcgatttc ctcagatcgc
     2521 ggcgcgagaa gctaagcccc aaggcggttg gccttccaac tgggcggcgg cgtcggactg
     2581 cgggcctgcg gcgcgaggag gtggccgaac tcgcggggat cggcgtcgac tggtatatcc
     2641 ggctggaaca gggacgtacc gtcagtccgt ccgtcaccac catcgatgcg ctggcgcgcg
     2701 cgctgcgcct tggcaaggcg gagcatgccc atttgcgggc tttggcctcg gatgccgatc
     2761 gccgcgcctt ctcccgcgaa aatgtccctg acgcgatccg gcgcctggtg gataacctca
     2821 atcagcccgc ctatatcacc ggccggcgct gggacgttct ggcgtggaat gcggctgcag
     2881 aggagatttt tgcgttcagc cgcttggcgg aagaggaccg caacaccctc atcagcgtgc
     2941 tgaccaatcc gcacacgcgc ggcctgttcg gagcgacctg ggccgacgag gccaaacgca
     3001 tggtcgccca atttcgtgcc acgcacgatc tttgggccgg cgatcccgcc tttctcagcc
     3061 tgctggagcg ccttcggcaa ggttgccccg agtttgcgat ctggtgggag gcgcacgaca
     3121 tcggcagcgt cacggctggc cgcaaattgc tgagccaccc caaaaagggc tcactgcgct
     3181 tcgaatacgc gacctttcag gccaatgacg atcccgcgct gaaactggtg atctacacgc
     3241 cggtgtgaga tgggcgccgc gtctagtaga cgcggcgatg ccagtagcgc ggtccgagtg
     3301 gctcgtcgcc cgcgtcggcg acgtagggat tattcacgca ctgggccgag cggcccgatg
     3361 ccgaggcgtt gcactgagcc agcgaattgt agcggcattc gtaggtccgt acacgtgcag
     3421 gcaaaccgga tagcccggag cgtacgtttg tgccgccgcc ggtcccgcga tcgaggccgc
     3481 cccgatcgcc acaatcgcca aaaccagact tcgcatcagt atgtccttcg ggatacgtgg
     3541 ccacgattgg gctgcagttc ggcattggcg ctgtaatagg gattggcgtc gcaataggcg
     3601 tcccggcccg aggcggtcgc ctggcactgc tgctagctcg aaaaactgca gtcgccgcgg
     3661 ccggcaccga aatcgcatcc ctcgatgcag aatgggaatt cccgcgccgc ggcgggcagc
     3721 ggacccgaag tcgagccggc tcagcacagt atcggcgccc aaaacacctt tcgcatggcg
     3781 tcctccgttc ccttcgagcc atttctcccg ctcgcgaaca gacaggaccg ggtggtccct
     3841 tcgggcgtga ggtgcgcagc ggaatttcag ggcgcggcac gatcagtaag acgggtagac
     3901 ggaccgtacg ctgtgtcggc tacgataggc gcgcggatcg ggctcgccgg gattgtaata
     3961 gggattttcc acacaataga gaaaccgccc cgaagcggtg gcctgacatt gctcatagct
     4021 tgtgaaggtg cagttgctga ggcccggata ctcgttgccc tgaatgcaaa acggatagcg
     4081 ggtgccaacg gccgcagccg gcgcggcgcc gatcatggcc aagccgcttg cggccagcaa
     4141 agccagaaca agattacgca tcacagtctc cttatcagcc gtagccgttc gttcattcgt
     4201 tgagagcaac ccaagccgga cgctggggtt ctcgtcaact cacattcgtg ggtgccggtg
     4261 cttcccacat ttgcgtactt attgccgaac acctcgaccg ggaacgcagc attgagcaag
     4321 aacaagtcga aagccgggca ggagcgctgt cagcggagac ggcttcacga ttcgacgatc
     4381 tgtatgtcct tggcgctgag cgacaagaga accgcttcaa tatcttccgg ctcaaggttg
     4441 gacgggatca attcattcaa ttggtcgaac gtaagggtgc cattcccttc gccaagcttt
     4501 attgcccttc ggatgacttc cgacaatttc atccagctct cccaagaagg ctctcactaa
     4561 caagaaggct ctcactaaga ggcgttcgac cctggccttg tggggcggaa aacaggcaac
     4621 ttccgaaatg aaaaccggcc atttgaaaga atttaccaaa ggggtcattc gcgggactgc
     4681 accacccttc ggcggcctct tgacttcttg gaacaaaaga ggaacaatgt tcttcttatg
     4741 ttccagtatc aggaggaagc catgttgaag acattcgtgg aagaggctgc ggcactgaca
     4801 tcgatcgcgc tttttgtcgc gatgattgcg atctgggcgc agttgattcc ccagctctga
     4861 ggccgcggcc agcgttggac ggggatgggg cagggcggcc ccacgccccg gctggggaaa
     4921 agcgggacgg cgatctggaa tcgcgcgttc cgcgtggaca cttcaggccc gaggcatcac
     4981 cattgagcgg cgagtcggcc ttcgaggccg cccgcgctcc ctcaagcccc agtgatcgct
     5041 ccagtccatg atcccaaaac cttcggcgac catgtccagt gccggatttg tccatctcca
     5101 cgtccattcg gcctattcgc tgctgaaggg atcgatcaag atccagaggc tcggcgagct
     5161 ggccaaggcg gaccgccagc cggcgctggc gctgaccgac accgacaaca tgttcggggc
     5221 gctggaattt tccgacaaga tggccggcta cggcatccag cccatcatcg gctgcgagct
     5281 ggcggtcgat ttcggcgatc aggacccgag cgcccgcaac gcgctggcgg cgggaccggc
     5341 gcggatcgtg ctgctggcag cgcgcgagcg cggctatcgc agcctgatgc ggctgaactc
     5401 gcgagccttt ctggagacgc cggtccatca gtcgccccac atcaagttcg aatggctgca
     5461 gggcgacgcc gaggacctga ttgcgctcac cggtggtccg gaggggccga tcgcgctggc
     5521 gatcaatgcc gaccaggcgg cgctggcggc gacgcgctgc gaccggctgg tgagcctgtt
     5581 cggcgaccgg ctttatatcg aattgcagcg ccacggcatc gagaaggaac gccgcgccga
     5641 agctggcctg atcgatctgg cctacgccaa gggattgccg ctggtcgcaa ccaacgagcc
     5701 gtatttcgca accgccgacg attacgaagc ccacgatgcg ctgctctgta tcgccggcgg
     5761 ccgtctcgtc gccgagaccg atcgcgatca attgaccccg gatcaccgct tcaagacccg
     5821 cgccgaaatg gcggtgctgt tcgccgatgt gccggaggcg ctggcatcca cgatcgagat
     5881 tgccgagcgc tgctcgttcc gccccgtcac gcgcaagccg atcctgccgc gcttcacggt
     5941 cggcgcgggc accaacgcgg cggaggcgga aagcgacgag gcggcggaac tgcgccgtca
     6001 ggccgaggaa ggcctgagca accggctcag ggttcacggc ctctcacaag gcagctccga
     6061 ggaggattac cgtgcccggc tcgctttcga gatcgatgtc atcacccgca tgaactatgc
     6121 gggctatttt ctgatcgtcg cggattttat ccaatgggcg aaggccgaag gcattccggt
     6181 cggaccgggc cgcggctccg gcgcgggctc gctcgtcgcc tgggcgctga ccatcaccga
     6241 tctcgacccg atccggttcg gcctgctgtt cgaacgcttt ctcaatcccg agcgggtgtc
     6301 gatgccggac ttcgacatcg atttctgcca ggatcggcgc ggcgaggtga tcgactacgt
     6361 gcagcagcgc tatggccgcg atcaggtcgc gcagatcatc accttcggaa cgctgcaggc
     6421 gcgcggcgtg ctgcgcgacg tcggccgcgt cttgcagatg ccttacgggc aggtcgacaa
     6481 actcacaaaa ctggtgccgc aaaatcccgc cgcgccggtg acgctttcag ccgccatcgc
     6541 aagcgagccg aaattgcagg catttcgcga tgaagacccg gtggttgcgc gggccttcga
     6601 catcgcccag cgcctcgagg gtctgacccg gcacgcgtcc acccatgccg ccggcattgt
     6661 gatcgccgat cgtccgctca gcgaactggt gccgctctac cgcgatccga aatccgacat
     6721 gccggtgacc cagttcaata tgaaatgggt cgagccggcg ggcctcgtca aattcgactt
     6781 cctcggcctc aagacgctga cggttctcga tgtcgccgtg aaactgctga ggcggcgcgg
     6841 ggtcgatgtc gatctcgcga ccctgccact cgacgatgcc accagttacc agatgctggc
     6901 gcgcggcgat gtagtcggcg tattccaggt cgaaagtcag ggcatgcggc gggcgctggt
     6961 cgatatgcgg cccgaccgct tcgaggacat catcgcgctg gtggcgctgt atcgtccggg
     7021 tccgatggcg aacattccga cctattgcgc ccgcaagcat ggcgacgagg agcctgaata
     7081 tctgcatccg atgctcgagc cgatcctgaa ggagaccttc ggcgtcatca tctaccagga
     7141 gcaggtgatg cagatcgcgc agctcatggc cggttattcg ctcggcgaag ccgacctgtt
     7201 gcgtcgtgcg atgggcaaga aaatccgctc ggagatggag aagcagcgcg cgggtttcgt
     7261 cgccggcgcg caacggaata acgtaccgaa agggcaggcg gataccatct ttgaactgct
     7321 cgcgaagttc gccgactacg gtttcaacaa gagccacgcc gcggcctatg ccctggtgtc
     7381 gtatcacacc gcctacatga aggcgcatta cccggttgaa ttcctggcag cgtcgatgac
     7441 gctcgacctc agcaacaccg acaagctgtc ggagtttcgc gccgaagcgc aacggctcgg
     7501 gatcaaggtc gaggcgccct cggtcaatcg ctcgggt
//
LOCUS       DASWGM010000234         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_710405,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000234 DASWGM010000000
VERSION     DASWGM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710405"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VGA15_07140"
     CDS             complement(<1..821)
                     /locus_tag="VGA15_07140"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9587506.1"
                     /translation="MRRVVVTGLGMVSPLGCGVEPTWKRILNSESGAKRIDTFDVSDL
                     TCRIACVIPRGDGTDGTFNPDQWMEPKDQRKVDDFIIFAMCAARQALDDANWHPATEE
                     DRCATGTMIGSGIGGLSGIADTALLLKERGPRKISPFFIPGRLINLASGYVSIEHGLK
                     GPNHAVVTACSTGAHAIGDASRLIALGDADVMVAGGTESPICRLSMAGFCASRALSTG
                     FNDAPEKASRPYDRDRDGFVMGEGAGIVVLEEYEHAKQRGAKIYAEVIGYGLSGDA"
     gene            complement(918..1157)
                     /locus_tag="VGA15_07145"
     CDS             complement(918..1157)
                     /locus_tag="VGA15_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9587507.1"
                     /translation="MSEIGERVKKIVVEHLGVEPEKVVDNASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAETILTVGDATKFLEKNAKS"
     gene            complement(1452..>1850)
                     /locus_tag="VGA15_07150"
     CDS             complement(1452..>1850)
                     /locus_tag="VGA15_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9587508.1"
                     /translation="ATFRLARAATRLMMRKRFGRIIAITSVVGVTGNPGQGNYTASKA
                     GLIGMIKTLGAEYAKRNITANCIAPGFVATPMTDALNDKQRETILSKVPAARLGTPED
                     IAAAAVYLSSNEAAYVTGQTIHVNGGMAMI"
BASE COUNT          348 a          603 c          517 g          382 t
ORIGIN      
        1 gcgtcgccgg ataacccgta accgatcacc tccgcataga tcttcgcgcc gcgctgcttg
       61 gcgtgctcgt attcttcaag caccacgatg ccggccccct cccccatcac gaaaccgtcg
      121 cgatcccggt cgtagggccg cgatgctttt tccggggcat cgttgaagcc ggtcgataac
      181 gcgcgggacg cgcagaaccc cgccatcgac agccggcaaa tcggcgattc ggttccgccg
      241 gccaccatca cgtcggcatc gccgagcgcg atcagccggc ttgcatcgcc gatcgcatgc
      301 gcgccagtcg agcaagcggt caccaccgca tggttgggac ctttcaggcc gtgctcgatc
      361 gacacataac ccgaggcaag attgatcaga cgcccgggaa tgaagaatgg cgatatcttg
      421 cgcgggccgc gctccttgag aagcagcgcc gtatcggcga tcccggagag gccgccgatc
      481 ccggagccga tcatcgtgcc ggtggcgcag cgatcctctt cggtggcagg atgccaattg
      541 gcgtcatcga gcgcctggcg cgccgcgcac atcgcgaaga tgatgaaatc gtcgaccttg
      601 cgctgatcct tcggctccat ccactggtcg ggattgaatg tgccgtcggt gccgtcgccc
      661 cgcggaatca cgcaggcgat ccgacaggtc agatcggaga catcgaatgt gtcgatcctt
      721 ttggcaccgc tctcgctgtt gaggatgcgc ttccaggtcg gctcgacgcc acagccgagt
      781 ggcgacacca tgccgaggcc cgtcacgaca acccgcctca tatccagcac tccagcccag
      841 gatttgccgc caacaacaag aaaccggtgg accgtacgac cacggcccgt ccggcttcct
      901 cggcgccacg caggggctca gcttttcgcg ttcttttcta gaaattttgt agcgtcgccg
      961 acggtcaaaa tcgtctccgc cgcatcatcg ggaatttcgc aaccaaattc ttcttcaaat
     1021 gccatcacga gctcgacggt atcgaggctg tcggcgccga ggtcgtcaat gaagctcgcg
     1081 ttgtcgacca ctttttcggg ttcaacacca aggtgttcga ccacaatctt cttaacccgc
     1141 tcgccaatct cactcatctt caacctcgtg cttgttccat tggactcgac cgcaagacgt
     1201 acgagccatc gtggtcgtta aacttccttc gcaatcgcgc atagtcttga tttggccccg
     1261 tcatagccga caaaggcccg gcgaacgcgg gcaggctccg catcgccaat atacagggtt
     1321 tcaaaatcct gcaatggcgt tctttgcctt ttccgtcgag ggttcggtta tcatacttcc
     1381 caagccttga ctataagcct caatcgggct tcggaagcct ttcgccccgc ttaggggtat
     1441 tggcgaccgg ctcaaatcat ggccatccca ccgttgacgt gaatggtctg cccggtgaca
     1501 tacgcagctt cattcgagct aagatagacc gccgccgcag cgatatcttc gggcgttccg
     1561 agtcgcgccg caggaacctt ggaaaggatg gtttcgcgct gcttgtcgtt aagtgcgtcg
     1621 gtcatcggcg tcgcaacaaa tcccggggcg atgcaattgg cggtgatgtt gcgtttggcg
     1681 tattcggccc ccagcgtctt gatcatgccg atcagtccgg ccttcgacgc cgtgtagttg
     1741 ccctgccccg gattgccggt cacgccaacg accgacgtga tcgcgatgat gcggccgaag
     1801 cgcttgcgca tcatcagcct ggtcgccgcc cgcgccaaac ggaaggtcgc
//
LOCUS       DASWGM010000235         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1127109,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000235 DASWGM010000000
VERSION     DASWGM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127109"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VGA15_07155"
     CDS             complement(<1..855)
                     /locus_tag="VGA15_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9587509.1"
                     /translation="MAMTMETAHGAHDAHDHPTGWRRYVYSTNHKDIGTMYLVFALVG
                     GVIGACLSIGIRLELMNPGLQIFSDVHTFNVFTTAHGLIMIFFMVMPAMIGGFGNWMV
                     PLMIGAPDMAFPRMNNISFWLLPASFTLLLTSLFVEGEPGGNGVGAGWTIYAPLSTSG
                     HPGPAVDFAILSLHLAGASSILGAINFITTIFNMRAPGMTLHKMPLFVWSILVTTFLL
                     LLAMPVLAGAITMLLTDRNFGTTFFNPEGGGDPILFQHLFWFFGHPEVYILILPGFGM
                     ISQVVATFS"
     gene            complement(904..1755)
                     /gene="coxB"
                     /locus_tag="VGA15_07160"
     CDS             complement(904..1755)
                     /gene="coxB"
                     /locus_tag="VGA15_07160"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9587510.1"
                     /translation="MLVLSRSIACVSALVVLTAASVALAALGHPTPWQTGLQDAGSPV
                     MENIIWFHNLLLIVITLITVVVLVLLVWVMVRFNQRAHPAPSRTTHRTSLEVAWTVIP
                     IAILVFIAVPSFRLLFLEQNIPPADVTVKATGKQWYWSYGYPDQGKFEFDSLMIQDKD
                     LKPDQPRLLSVDNEMVVPVDKIVRVQVTGSDVIHSFAVPSFGIKIDAIPGRLNETWFK
                     ATKEGIYYGQCSELCGKNHAFMPIAVRVVSDSDFNAWLEDAKKKYARGDDSLPRALAA
                     RESAKAE"
     gene            2125..2655
                     /locus_tag="VGA15_07165"
     CDS             2125..2655
                     /locus_tag="VGA15_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX9587511.1"
                     /translation="MGIVRQAKPRRAASFGLALAAVLACAGLPETAAAQGVVKSIYGD
                     WQIRCDTPPGAQSEQCALIQSVTAEDRPSVGLTVIVLKTADQKSRLMRVLAPLGVLLP
                     SGLGLKINQADIGRAGFVRCLPNGCVAEVIMDDNLIKQLRTGQTATFIIFQTPEEGIG
                     IPMSLKGFGDGFDKLP"
BASE COUNT          561 a          929 c          969 g          478 t
ORIGIN      
        1 gctgaaggtg gcgacgacct gactgatcat gccgaagccg ggcaggatca ggatgtagac
       61 ttcgggatga ccgaagaacc agaacaggtg ctggaacagg atcggatcgc cgccgccctc
      121 gggattgaag aaggtggtgc cgaaattgcg gtcggtcagc agcatggtta tggcgccggc
      181 gagcaccggc atggcaagca gcagcaggaa cgtggtcacc aggatcgacc acacgaacag
      241 cggcattttg tgcagggtca tgcccggcgc acgcatgttg aagatcgtgg tgatgaagtt
      301 gatggcaccg agaatcgagg aggcgccggc aaggtgcagc gacaggatgg caaaatcgac
      361 cgccgggccc gggtggccgc tggtcgagag cggcgcataa atcgtccagc ccgcccccac
      421 gccgttgccg ccgggctcgc cttcgacgaa cagcgaggtc agcagcaagg tgaaggaagc
      481 cggcagcagc cagaacgaaa tgttgttcat gcggggaaac gccatgtccg gcgcgccgat
      541 catcagcgga accatccaat tgccgaaccc gccgatcatg gccggcatga ccatgaagaa
      601 gatcatgatc aggccgtgcg cggtggtgaa cacgttgaac gtgtgcacgt cggagaaaat
      661 ctgcagcccg ggattcatca gctcgagacg aatgccgatc gacaggcaag cgccgatcac
      721 cccgccgacc agggcgaaca ccaggtacat cgtgccgatg tccttgtggt tggtggaata
      781 gacatagcgg cgccatccgg ttggatgatc gtgcgcatcg tgggcgccgt gagccgtctc
      841 catcgtcatt gccatgtcgg atccctcgac ctcgctgccg gaccgcggtg cggccccggt
      901 ctcctattcc gctttcgccg attctctcgc ggccaatgcc ctcggcaggg aatcatcgcc
      961 gcgcgcgtat ttcttcttgg cgtcttcgag ccaggcgttg aaatcgctgt cgctcaccac
     1021 gcgtaccgcg atcggcatga acgcgtggtt cttgccgcaa agctcggaac attgcccgta
     1081 atagattccc tccttggtgg ccttgaacca ggtctcgttg agccgccccg gaatggcgtc
     1141 gatcttgatg ccgaacgagg gcaccgcgaa ggaatggatc acgtcggacc cggtcacctg
     1201 cacgcggacg atcttgtcga ccgggaccac catttcattg tcgaccgaga gcagccgcgg
     1261 ctggtcgggc ttgaggtcct tgtcctggat catcagcgag tcgaactcga acttgccctg
     1321 atcgggatag ccgtagctcc agtaccattg cttgccggtg gccttcacgg tgacatccgc
     1381 cggcgggatg ttctgctcga ggaacaacag gcggaacgac ggcaccgcga tgaacaccag
     1441 gatggcgatc ggaatcaccg tccaggccac ctcgagggag gtgcgatgtg tggtgcgcga
     1501 cggcgcggga tgggcacgct ggttgaaccg caccatcacc cacaccagca gcacgagcac
     1561 gaccacggtg atcaaggtga tgacgatcag cagcaagttg tggaaccaga tgatgttttc
     1621 catcaccggc gagccggcgt cctgcagtcc ggtctgccac ggcgtggggt gccccagcgc
     1681 ggcaagagca acgcttgcag ccgtgagcac gaccaaggcc gacacgcagg cgatcgaccg
     1741 cgacagcacc agcatcgttc gactagctcc ttacgacggg ttaccggcgg ggccggtcgc
     1801 gctcgctcgg ctatgagcgc tcgggcttgc ccgctcgggc gctcggtgcc gcgggatgtt
     1861 ggcgcgaggg tgaagcgtcc gggcggaggc caagcttctc aaaccacaat tccgccctac
     1921 tcgcaatgtg ccttcgcgcg ccgccggaga ccggctggga agcgcgcggc cgcggctcga
     1981 accggccttg cggcaggttg cggatgtttc ttgacagaac caaggcgcca tgatccgaga
     2041 gcgccccaag aggggcggcg ccggccgcga atcgatgaaa ggcgcgacat cgtccgcggg
     2101 tcagcccggt cgaggtttaa gctgatgggg atcgtccgcc aggcaaagcc gcggcgcgcc
     2161 gcgagttttg gtctcgcgct cgccgccgtg ctggcgtgcg ccgggttgcc ggagacggcc
     2221 gcggcgcagg gcgtcgtgaa atcgatctac ggcgattggc agatccgctg cgacacgccg
     2281 cccggagcgc aaagcgagca atgcgcgctg atccagagcg tcaccgccga ggatcgcccc
     2341 agcgtcgggc tgaccgtcat cgtgctcaag accgccgatc agaaaagcag gctgatgcgg
     2401 gtgctcgcgc cgctgggcgt gctgctgcct tccgggctcg ggctgaagat caaccaggcc
     2461 gacatcggcc gggccgggtt cgtgcgttgc ctgcccaacg gctgcgtcgc cgaagtgatc
     2521 atggacgaca atctgatcaa gcaattgcgc accggccaga ccgcgacctt catcattttc
     2581 cagactcccg aagagggcat cggcattccg atgagcctca aggggttcgg cgacggattc
     2641 gacaagctgc catgaggctc ttacctcccc gcaggggagg tcggcgagca tcgcaagatg
     2701 cgagcggggt ggggcggacc ccaccccggc agctcgcaat gctcgctgcc ggccctcccc
     2761 gttccgggca gggagaagaa cccctcgccg cctcgccttc gcagccctat gtttgggaaa
     2821 ccccgtatcg gaaccggcca tgacccaagt ctcgacggcc tcgctcatcg accgcaccgg
     2881 gctcgaccga gtccggctca agcgccacct cgcccgcggc ttcgaaggcg ccgacga
//
LOCUS       DASWGM010000236         4743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1539755,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000236 DASWGM010000000
VERSION     DASWGM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539755"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VGA15_07170"
     CDS             complement(<1..666)
                     /locus_tag="VGA15_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX9587512.1"
                     /translation="MPINADYRGPGDLPEVIPVFPLPGALLLPRGQMPLNIFEPRYLA
                     MVDDALRDGHRLIGMIQPDVSHSQNEARPALFRVGCVGRITQLAEAGDGRYILELTGI
                     ARFKVVEEIAVLTAYRQCKVDYFPYIDDFIARKGEEAVDREALLEVLTDFLKANNLKV
                     DWEGVESAPNEALVNALAMMSPYGPPEKQAMLEAPDLKTRAEILIAVTEMDLAKKRTS
                     GDTG"
     gene            complement(751..1674)
                     /gene="trxA"
                     /locus_tag="VGA15_07175"
     CDS             complement(751..1674)
                     /gene="trxA"
                     /locus_tag="VGA15_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9587513.1"
                     /translation="MTIVEQGSVPAPQAAPDLIKETTTQTFVKDVIEESKRQPVLIDF
                     WAPWCGPCRQLTPIIEKAVRAAKGKVKLVKMNIDEHPAIPGQMGIQSIPAVIAFVNGQ
                     PADGFMGAVPESQVTAFIEKLTASMPGAGEPNIAEILKEAEAVLAEGDPATAASIYAE
                     VLAADATNIAGLAGLAKCYMATGAIEQAKQTIAMVPESKRNDAGVKAVQASIDLAEQA
                     KALGPVTELEQKVAANPLDHQARFDLATALNASGKRAEATNQLLEIVKRDRKWNEDGA
                     RKQLVQFFEAWGPTDEATVDGRKRLSTILFS"
     gene            1915..2415
                     /locus_tag="VGA15_07180"
     CDS             1915..2415
                     /locus_tag="VGA15_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9587514.1"
                     /translation="MAKAYYSTVFEQPAPEVWKIVRDFNNYPIWVGGAGESKIEDGKS
                     GDTVGAVRRVLYQDGHIRQRLLAQSDVERSQTYEFCGAASLPVTGFQATLRITPIVDG
                     DRAFVEWWATFDCDAPRREELTGTLQGWFGKWLESLRSTLGSRPSGFPEAASGEVGDR
                     SQAVSG"
     gene            2548..2622
                     /locus_tag="VGA15_07185"
     tRNA            2548..2622
                     /locus_tag="VGA15_07185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2580..2582,aa:Gly,seq:gcc)
     gene            2826..4169
                     /locus_tag="VGA15_07190"
     CDS             2826..4169
                     /locus_tag="VGA15_07190"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEX9587515.1"
                     /translation="MSRNIVLLSDGTGNSAAKVWRTNVWRTFGALDLSGNDQVAFYDD
                     GVGTSTFKPWAILGGAFGFGLKRNVIDIYKFACRNYKDETDDIYGFGFSRGAFTIRVV
                     IGLILNQGLVSADNEAELDKKAIAAYRKYRSERYHTLWPWHPEDWYRAIRNALLPIKY
                     DKRDNRPVKHIRFVGVWDTVAAYGLPIDEMTRGVSRWIVPLELPTHTLDRERVMRACQ
                     ALSLDEDRTTFHPELWDEKVVPPSKFDPDKKRQIADEQISQVWFAGVHSNVGGGYPDD
                     ALAYIPLVWMLTEAQRCGLKLKSDVGDPPADPDALKNAISMGDKDGRIYDPRAGLGGY
                     YRYGPRKLVQLCNYKYSKKEDDEVTIERPKIHETVFRRTETMRTPTRPSGCPPPTTSS
                     RATAKLSRPINMASRPATLQRPARTRKNISGMKSGSAASSISQPSAQPHGYWRSR"
     gene            4349..>4743
                     /locus_tag="VGA15_07195"
     CDS             4349..>4743
                     /locus_tag="VGA15_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587516.1"
                     /translation="MTIAARISDRKGAIWRKTSGAPSGLPDDGIYRLRSNRIYIWFHE
                     GLKRRWAPALFALLFVYLGLSFLSHGLYDIQDVAGLTCVESTDAKKLEKPEWSDKAKD
                     KDGQPIIATATASFEISELCNPTKILLEKG"
BASE COUNT          880 a         1464 c         1439 g          960 t
ORIGIN      
        1 ccccgtatcg ccgctggtgc gtttcttggc cagatccatc tcggtgaccg cgatcagaat
       61 ttcggcgcgg gtcttgagat cgggcgcctc aagcatcgcc tgcttttccg gcgggccata
      121 gggcgacatc atcgccagcg cgttgaccag cgcctcgttg ggcgcgctct cgacgccttc
      181 ccagtcgacc ttgagattgt tggctttgag aaaatcggtc aacacctcga gcagcgcttc
      241 gcggtcgacc gcttcttcgc ccttgcgcgc gatgaaatcg tcgatgtagg ggaaataatc
      301 caccttgcat tgccgatacg cggtcagcac ggcaatttcc tcgaccacct tgaagcgcgc
      361 gatcccggta agctcgagaa tgtagcggcc gtcgccggcc tcggcgagct gggtgatgcg
      421 cccgacgcag ccgacgcgaa acaacgccgg cctggcctcg ttctgcgaat gggaaacatc
      481 cggctggatc attccgatca gccgatggcc gtcgcgcagc gcgtcgtcca ccatcgccag
      541 atagcgcggc tcaaagatgt tgagcggcat ctgaccgcgc ggcagcagca gcgcgccggg
      601 cagcggaaac accgggatca cctcgggaag gtcgccgggc ccgcgataat cggcattgat
      661 cggcatgtgc ggttccggcg ctaatccagg ggttctaaac ttgcgcgcat cctaatcgca
      721 aaaccggcgt ccgcttttgc ggaacccgcg ctatgagaac aagattgtcg atagccgctt
      781 tcgtccatcc acggtggctt catcggtcgg accccacgcc tcgaagaact gcaccaattg
      841 ctttcgcgcg ccatcctcgt tccatttacg gtcgcgcttg acgatttcga gcaactggtt
      901 ggtggcctcg gcgcgcttgc cgctggcgtt gagcgcggtc gcaagatcga atcgcgcctg
      961 atgatcgagc ggatttgcgg cgactttctg ctcgagctcg gtcaccgggc cgagcgcctt
     1021 ggcctgctcg gcaagatcga tcgacgcctg caccgccttg acaccggcgt cattgcgttt
     1081 cgattccggc accatcgcga tggtttgctt ggcctgctcg atggcgccgg tcgccatgta
     1141 gcatttcgcg agccctgcta gccccgcgat attggtggcg tccgcggcca gcacctcggc
     1201 atagatcgat gccgcggtgg cgggatcgcc ttcggccaaa acggcttcgg cctccttcag
     1261 gatttccgcg atattgggct cgcccgcgcc cggcatgctc gccgtaagct tctcgatgaa
     1321 ggcggtgacc tggctttccg gaaccgcgcc catgaaacca tcggcgggct ggccgttgac
     1381 gaaagcgatc acggccggga tcgactggat tcccatctgg ccgggaatcg ccggatgttc
     1441 gtcgatgttc atcttgacca gcttgacctt gccctttgcc gcccggaccg ccttctcgat
     1501 gatcggcgtg agctggcggc aggggccgca ccacggcgcc cagaagtcga tcagcaccgg
     1561 ctggcgcttc gattcctcga tgacgtcctt cacgaacgtc tgggtggtcg tttccttgat
     1621 caaatcgggc gccgcctgcg gcgccggcac gctgccctgc tctactatcg tcacgggaat
     1681 cctcgcctga tgtatcggga tcggggccta ttctagcacg ccccagggtg gtggttgaga
     1741 agttctctgc acctaagtgg cggtttgccg ggaaatttca atcggttcca ggtgtggtcg
     1801 gaagcctcgg atgtattggc cgcaacgggt tcgctcggtc ggggatcggg tgggcggcac
     1861 gggtttcctc gcccatggtc cggtttcaac cgggtccaat gaagggattg agagatggcc
     1921 aaagcctatt acagcaccgt gttcgaacag cccgcccccg aggtgtggaa aatcgttcgc
     1981 gatttcaaca actatccgat ctgggtcggc ggcgccggcg aaagcaagat cgaggacggc
     2041 aagtctggcg atacggtcgg ggcggtcagg cgtgtgctgt atcaagacgg gcacattcgg
     2101 caacggctgt tggcgcaatc cgacgtcgag cggtcgcaaa cttacgaatt ctgcggcgcg
     2161 gcatcgttgc cggtcaccgg ctttcaggcc accttgcgca tcacgcccat cgtcgatggc
     2221 gaccgggcct tcgtcgaatg gtgggcgacc tttgattgcg acgcgccccg tcgcgaagaa
     2281 ctgaccggga cccttcaggg ctggttcggc aaatggctcg aatcgctccg cagtacgctg
     2341 ggcagccggc cttcggggtt cccggaagct gcgtccggtg aggtcggcga ccgttcgcag
     2401 gccgtttcgg gctgatttgc ccgcaatcgc tccgatatcg ggcgattggg ccggttcaaa
     2461 ggccacgcgc agcctgttgc attcgcggtg cgcatttggc atagctctgc ccgttgccgg
     2521 cgcgccatcg ccgccaatct ctcggatgcg ggtgtagctc agtggtagag cacgaccttg
     2581 ccaaggtcgg ggtcgagggt tcgaatccct tcgcccgctc cagaattttt ttaggtggca
     2641 aagcgacagt tcaacgacaa gccgccgctc ggcggctttg ttgtttttcg tccattcgct
     2701 tgcaaatcgt cggcctgcgc gattcctgat caccgattgt gaaaattttc atactcggcg
     2761 ggggcatgca atgttctaaa actataggtt gcaaagtagt tcgacaacac ggcggggggg
     2821 attccgtgag tcgaaacatc gtgctgcttt ccgacggcac aggcaattcc gcggcgaagg
     2881 tgtggcgcac aaatgtctgg cgcaccttcg gggcgttgga cctgtcggga aatgaccagg
     2941 tcgcattcta cgacgacggc gtaggcacct cgacgttcaa gccgtgggcg attcttggtg
     3001 gtgcgttcgg cttcggcctg aagcggaacg tcatcgacat ctacaaattc gcctgccgca
     3061 actacaagga tgagaccgac gatatctatg gcttcggctt cagtcgtggc gccttcacga
     3121 tccgcgtggt catcggcctg atcctcaatc agggcctggt gtccgctgac aatgaagccg
     3181 agctcgacaa aaaggcgatc gccgcctacc gaaagtatcg cagtgagcgc taccatacgc
     3241 tgtggccgtg gcatccagag gactggtacc gggcaattcg gaacgcgcta ctgccgatca
     3301 agtacgacaa gcgcgacaat cgccccgtca agcacatccg cttcgtcggg gtgtgggaca
     3361 cggtggccgc ctacggtctg ccgatcgacg agatgacgcg cggcgtcagc cgctggatcg
     3421 tgccgctcga acttcccact cacaccctcg atcgcgaacg cgtgatgcga gcctgccagg
     3481 cgctgtcgct tgatgaagat cgcaccacgt tccatcccga gctgtgggac gaaaaagtcg
     3541 ttccgccttc gaagttcgat ccggacaaaa agcgtcagat cgccgatgag cagatcagcc
     3601 aggtatggtt cgccggtgtc cattccaacg tcggcggcgg ttaccccgat gacgcgctgg
     3661 cctacatccc gctggtctgg atgctcaccg aggcgcaacg ctgcggcctc aaactcaagt
     3721 ctgatgttgg ggatccgccc gccgatcccg atgcgttaaa aaatgccatc tcgatgggtg
     3781 acaaggacgg acgaatctac gatccgcgtg cgggacttgg cggctattac cgctacggcc
     3841 cgcgcaagct ggttcagctc tgcaattaca aatattcgaa aaaggaagac gacgaagtca
     3901 cgatcgagcg tccgaaaatt cacgaaaccg tcttcaggcg gacggaaacc atgcgcacgc
     3961 ctacgcgccc atcgggctgc ccgccaccta cgacatcgtc aagagcaacg gcgaaattgt
     4021 cacgcccgat caatatggct tcgagaccgg cgacgctgca aaggcccgcg cggacgcgca
     4081 agaacatatc tggaatgaaa tctggaagcg ccgcatcgtc tatttcgcaa ccgtcggcgc
     4141 aaccgcatgg ttactggcgt tcccgctgat cagtggagcg caacgcgccg acgagttcaa
     4201 gagcccggtc cggtggatct cggacatcat ccgccttgtc ggcgggttcc cgccaggctt
     4261 cgcctcgacc tgggtcgacg gctatgcccg tgcgccgatc tccttcgtct tgctggctct
     4321 gctcgtggcc tccttcacca tatggggaat gacaatcgcc gcgcgcattt cggatcgcaa
     4381 gggtgcgatc tggcggaaaa cgtccggcgc gccatcgggt ttgccggacg acggcattta
     4441 ccgtctgcgc agcaatcgga tctacatctg gtttcatgag ggcctgaagc gccgctgggc
     4501 gccggccctg tttgcgctgc tgttcgtgta cctcggactc tcttttctca gccacggcct
     4561 ctacgatatc caggacgtcg cgggccttac ctgcgtcgag agcaccgacg ccaaaaaact
     4621 cgaaaagccc gaatggtcag acaaagccaa agacaaagat ggccaaccga tcatcgcgac
     4681 cgcaaccgca tccttcgaga tctcggagct ctgcaatccg acgaaaatcc ttcttgagaa
     4741 ggg
//
LOCUS       DASWGM010000237         7920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1820801,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000237 DASWGM010000000
VERSION     DASWGM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1820801"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..143
                     /locus_tag="VGA15_07200"
     CDS             <1..143
                     /locus_tag="VGA15_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9587517.1"
                     /translation="KPAEFKIYPGAPHGFHADYRASYRKEAADDAWSQMQTWLKKYKV
                     LG"
     gene            complement(515..1033)
                     /locus_tag="VGA15_07205"
     CDS             complement(515..1033)
                     /locus_tag="VGA15_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587518.1"
                     /translation="MLRKTMIALLAATSVAILAPGAASARGGGGGGGGGGGHGGGGFG
                     GGSFHGGGAMAFHGGGMGAGVGGGGFRSAAIGGGVGGGGLQGRGWEGRGFEGHGFRSD
                     GFHHHGFHHGRGFGVGVFGYGPYAYYGDDYYDYPDYAYDDSYYDNGDCYVVQRRIHTR
                     YGWRLRPVQVCN"
     gene            complement(1293..2936)
                     /locus_tag="VGA15_07210"
     CDS             complement(1293..2936)
                     /locus_tag="VGA15_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX9587519.1"
                     /translation="MNFARRVDDLGVAEKIYSIVALLVILTTFLVVMSIQSVRLQTAY
                     RHLLASSASAAINIERVNGLIYAIVMESRGIYMSTERTKVKQFGDELLKRNRELAEVV
                     AGWEETVRFDDAAQFSAFKQRIIQFIEFRKELVRRAMQISPAAGREWGDNDANRALRS
                     QLNADLEAFARIYADRAGDVAELDDKSRYASWYLFALGLGALMLAALNVFVMRRYVTG
                     PLSQITEATALIAAGKIELDIPFVARKDEIGHLARAVRNFRDAACRNLELEQLEIGTA
                     KQRDTAMGERDKLTDKYHATKWQLSAAINSMPQGMIMLDAAAKVLAVNDQYRKIYGLP
                     PTIKTGSSLQEILQHRAENGLFAGNVARDLVAVVARIAKRQPSTDEILLGDGRVVSIR
                     ERPMDGGGVVAVHDDITEQRRSQRILERTEQFLATIIENVPQAIVAKDARNLRYVLVN
                     RAAEAMIGMSRAEIMGKTARELFSAETAELIERRDRQLLGQEHQLDAIVDTMDNPVKG
                     RRTVAIRRLQVGGPDRESHLFVSMIEDRTDQANVAGAAA"
     gene            3177..3419
                     /locus_tag="VGA15_07215"
     CDS             3177..3419
                     /locus_tag="VGA15_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587520.1"
                     /translation="MRKLIAFDDDTFDKLKQLGRDRMATVQELADEAFADLLKKHGIP
                     VDLKDALHKSASLAMTPAKTPAESHVKSSPSRKSRS"
     gene            3416..3583
                     /locus_tag="VGA15_07220"
     CDS             3416..3583
                     /locus_tag="VGA15_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587521.1"
                     /translation="MTTDPDPFEEGQRAARENIPAEGNPYQDGSQEHARWAAGHEEAA
                     GEIEADGSEGN"
     gene            complement(3625..3777)
                     /locus_tag="VGA15_07225"
     CDS             complement(3625..3777)
                     /locus_tag="VGA15_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587522.1"
                     /translation="MMTMMTVPQMTVIFLATVVCLGAAFVWMFWELQKPAQLARRSDR
                     FERANE"
     gene            complement(3842..4225)
                     /locus_tag="VGA15_07230"
     CDS             complement(3842..4225)
                     /locus_tag="VGA15_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX9587523.1"
                     /translation="MPLARIDLAKGKPLDYRKTIGEVVYDAMVETLKAPKDDRFQIIT
                     EHAGDDFIVDPSYLGIQRSKDCVIVQLTLNEGRTVDQKRAFYKAVATGLHQRLNLRPQ
                     DVFISLVEVKKENWSFGNGEAQYAS"
     gene            complement(4307..4705)
                     /locus_tag="VGA15_07235"
     CDS             complement(4307..4705)
                     /locus_tag="VGA15_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX9587524.1"
                     /translation="MERPMPFIRITVSGPTLASNQVSLLQVEMTELMASVLGKRADLT
                     SVLVEQAASAGWAIGGTLAKIAVHVDATITAGTNSAEEKARFIEKTMRLLKSVLGSEL
                     HPATYVVVTEVPANAWGYDGRTQESRQLAA"
     gene            4868..5719
                     /locus_tag="VGA15_07240"
     CDS             4868..5719
                     /locus_tag="VGA15_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9587525.1"
                     /translation="MNRAAEQVGRSQSAVSQQMRKLEEKIGQRLFRKQGRGLMLTEPG
                     ELLLKYARRILALNDEAIAALRGSGLEGVVRFGLPSDFAETWLPAALGQFKRAHPLIR
                     VEASVDRNTVLIERLEKGRLDLAMFFSDGQHQGAERLATLPMVWIGSAEHAWDPTEPI
                     PLVLFEAPCVFRKAGIAALDAAGIPWRIAFTSPSLAGLWAAVEAGLGISPRTTAHLPD
                     WLKVLGKAYKLPKLPSIDLSLYDLGRPLMPAAMRLKEILTDTIPPGLSELRRASHSGR
                     RGGSAAA"
     gene            complement(5780..6310)
                     /locus_tag="VGA15_07245"
     CDS             complement(5780..6310)
                     /locus_tag="VGA15_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX9587526.1"
                     /translation="MAIYELDGQAPELPADGNYFIADTAMVIGKVRLHNSASVWFGAV
                     LRGDNEWIEIGENANVQDNSTCHTDLGFPLTIGKNCTVGHNVILHGCTLEEGALVGMG
                     SIVMNGARIGRGSIVGAGSVITEGKQFPEHSLIIGSPARVIRTLDPEQVARMGSAARF
                     YVGNGPRFKKGLKKIG"
     gene            complement(6353..6682)
                     /locus_tag="VGA15_07250"
     CDS             complement(6353..6682)
                     /locus_tag="VGA15_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587527.1"
                     /translation="MSPDALNSLFSLCIGFALAGALVSGYQALVRRPAGFGLLQDGVA
                     PKTFAAVPFLVFAAPFIIMRNTLRGARIERRRVEFVMMATVLAGFWSLMSGTFFVMTL
                     RAAGVLA"
     gene            6907..7617
                     /locus_tag="VGA15_07255"
     CDS             6907..7617
                     /locus_tag="VGA15_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587528.1"
                     /translation="MTLFSTAQHLARGPRRDLRGHMMAIMLAGAATTGAIALVAYLLW
                     PTWGPDPASSPARLPVSIGATLFNVPTAAVRMKIQRHSGPQERIDLSFAFPSLEAADG
                     PRHVSADTVAQAIQPIDRIFLSISAHHDTLAPDLRVSTIYPRYLEQALQPGQDGLTMR
                     AFRDGTPYSNEDLFLASTPTLSARCTRDAATPGMCLSERRIDGADLTFRFPRSWLAQW
                     RDVADAVERLTLQLHGAT"
     gene            complement(7786..>7920)
                     /locus_tag="VGA15_07260"
     CDS             complement(7786..>7920)
                     /locus_tag="VGA15_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HEX9587529.1"
                     /translation="KKDDSAEVYIGEEFIGVLFVDDEDDDRSFQFQMAILEDDLVEQG
                     "
BASE COUNT         1572 a         2484 c         2350 g         1514 t
ORIGIN      
        1 agaagcccgc cgaattcaag atctatccgg gcgcgccaca cggcttccat gccgattatc
       61 gcgcaagcta tcgcaaggaa gcggccgacg atgcatggag ccagatgcaa acctggctca
      121 agaagtacaa agtgctgggc taatacatgc aagctcgccg agcgcgctcg gcctcgcgcc
      181 gcgcgcgctc ttcccaaggc caaagatgcc ggtcagccta tccgaaccga tcgctgttgc
      241 gacgcggttt ggacgtttgc tcgacctccc agagcatcca aacaaaggca atgcccagac
      301 cgacgacgac gcccaaaaat accaacgtca ggcccggggc tgtgagcatg gtgaacatcc
      361 gtagatgaat gcaagcggcg taccagtgcg tggcgtcaga aaaacggcct tggtgcagga
      421 tacaccaagg ccgcgccaat cttgccggcc gaggccggtg ctcaacttta tttgaccggt
      481 accccgacta ccagatgatc agccgcccca atggtcagtt gcacacctgg accgggcgaa
      541 ggcgccagcc gtagcgcgtg tggatgcgcc gctgcaccac gtagcagtcg ccattgtcgt
      601 aataggaatc gtcgtaggcg tagtcgggat aatcataata gtcgtcgccg taataggcgt
      661 acggtccata gccaaatacg cccactccaa atccgcggcc gtggtgaaac ccgtggtggt
      721 ggaagccatc gctgcggaaa ccgtggccct cgaaacctcg gccctcccaa ccccggcctt
      781 gaagtccacc gcctccgaca ccgcctccga tagcggctga acggaaacct ccgcctccga
      841 cgccggcgcc catcccgccg ccatgaaagg ccatcgcgcc gccgccatga aagcttcctc
      901 cgccaaaacc accgccgccg tggcctccgc caccgccgcc acctccgccg ccgccacgag
      961 ccgaggcggc gcccggcgcc aggatggcaa ccgaggtggc tgccagtaaa gctatcatcg
     1021 ttttgcgcaa cataattgta ctccctagga ttgactgctc ccagcaacta gacgcgtcca
     1081 gcaggagata gtttcagcct caggggaagt tgataggccg tcgcagacct ttctataaat
     1141 tccgatttag actcggcggg ttcgggaacg cggtgaaaat gtgttgtcgc cgcagcgaag
     1201 gcggcagacg accacgccga cctccgtgcg agcgccgcaa cgcacaagca ccaagcgagg
     1261 cgtcaaaacc ggctcgggca gccgtggcgt gcttacgccg cggcacccgc cacattggcc
     1321 tggtcggtcc ggtcctcgat catgctgacg aagaggtggg actcgcggtc cgggccaccg
     1381 acctgaagcc gccgtatggc gaccgtgcgc cggcccttga cgggattgtc catcgtgtca
     1441 acgatggcat cgagttgatg ttcctgtccc agcagctgcc ggtcccgtcg ctcgatcaat
     1501 tcggcggtct ccgcggaaaa cagctcacgc gccgtcttcc ccatgatctc ggcgcgggac
     1561 atgccgatca tcgcttcggc cgcccggttg accaaaacgt agcgcaggtt acgcgcgtcc
     1621 ttggcaacaa tcgcctgggg cacattctcg atgatcgtcg ccagaaactg ctcggtccgc
     1681 tccaggatcc gctgactccg ccgttgctcg gtaatgtcgt catggactgc caccacgccg
     1741 ccgccatcca ttggccgctc ccggatgctg accacacgac cgtcgcccag taaaatttca
     1801 tcggtcgaag gctgccgctt cgcgatccgc gcgacgaccg caacgagatc cctggcgacg
     1861 ttcccggcaa acagtccgtt ctcggcccgg tgctgcagga tctcctggag cgaagaaccg
     1921 gtcttgatgg tcggtggcag cccgtaaatt ttccggtact ggtcgttgac cgcgagaacc
     1981 ttcgccgcgg cgtcaagcat gatcatgccc tgcggcatgc tgttgatcgc cgcggacaat
     2041 tgccacttgg tcgcgtggta tttgtcggta agcttgtcgc gttctcccat cgcggtatcg
     2101 cgctgcttgg ccgtgccgat ttcgagttgc tcaagctcga ggttacggca ggccgcgtct
     2161 cgaaagtttc gcacggcacg ggcgagatgt ccgatctcgt ctttgcgggc gacgaaggga
     2221 atatcgagtt cgattttccc ggccgcaatc agagccgtcg cctcggtgat ttgagacaat
     2281 ggcccggtga cgtagcgtct catgacgaag acgttcaggg ccgcgagcat caaggcgcca
     2341 aggccaaggg cgaacaggta ccacgaagcg taccggcttt tgtcgtccag ttccgcaacg
     2401 tcgccggcgc ggtccgcgta aatccttgca aaagcctcga ggtcggcgtt gagctggctg
     2461 cgcaaggcgc ggttggcgtc gttgtcgccc cactcgcgcc cggccgccgg actgatctgc
     2521 atcgcacgcc gaaccaactc tttgcggaat tcgatgaatt ggataattcg ctgcttgaag
     2581 gcagaaaact gcgccgcatc gtcgaagcga actgtttcct cccatcccgc tacgacctcg
     2641 gcgagttcgc gattgcgctt gagaagttca tcgccaaatt gcttgacctt ggtccgttcg
     2701 gtggacatgt agatgccgcg cgattccatg acgatcgcat agatcaggcc gttaacccgc
     2761 tcgatattga ttgcagccga agcggatgat gccagcagat gccgatacgc ggtttgcagc
     2821 cgcaccgatt gaatcgacat caccacaaga aaggtcgtaa ggatgaccag caacgcgacg
     2881 atgctataga tcttctctgc gacgccgaga tcatccaccc gcctggcgaa attcaccgca
     2941 atccttctga tgcttccgcc tccgggcatt gcaccgggtc cggcattcgg gattgcgtcc
     3001 atcatcgaac tcgttcgcag gagggtttgt ttgagtctta ggccgcgcag cgctattgtg
     3061 accttaattc cgctacccgt attactacgg agcgatccgg tgccgaccca aggaactatt
     3121 cctatccggt ccgctttagg tcttggcaaa gacgttggcg caggagcaga cgacgcgtgc
     3181 gcaagttgat cgccttcgac gacgacacct tcgacaagct gaagcagctt gggcgcgacc
     3241 ggatggcgac ggttcaggaa ctggccgacg aagcgttcgc cgatctgctc aagaagcacg
     3301 gcatcccggt cgacctgaag gacgcgcttc acaagagcgc atcgctcgcc atgacaccgg
     3361 ccaagacacc ggcggaaagc catgtaaaat cgagtccatc gaggaaatcg agatcatgac
     3421 gaccgatcca gatccgttcg aggaaggcca gcgcgccgcg cgggagaaca ttccggctga
     3481 gggcaatccg tatcaggacg gcagccagga acacgcgcgc tgggctgccg gccatgagga
     3541 agccgccggc gagattgaag ccgatggatc ggaaggcaat tgaagcgcgc gccatctacg
     3601 gcgcgcaaat tcgcgggcag gccttcattc gttagctcgc tcgaaacgat cgctgcggcg
     3661 cgcaagttgc gccggcttct gcaattccca gaacatccag acaaatgccg cgccaaggca
     3721 aaccaccgtg gccagaaata tgaccgtcat ctggggtact gtcatcatcg tcatcatggg
     3781 ttaggtccat gcacacgcct tgccttaagg tccttgcgca gatgcaatgc tccgctgagc
     3841 cctacgaggc gtattgcgcc tctccgttac cgaacgacca gttttccttc ttcacctcga
     3901 caagactgat gaacacgtcc tgcgggcgga gattcaggcg ttgatgcaat cccgtcgcaa
     3961 cggccttgta gaaggcgcgc ttttgatcga ccgttcgacc ttcgttaagc gtcaactgaa
     4021 caatcacgca gtcctttgat ctctggatgc ccagataact gggatcgacg atgaaatcgt
     4081 caccggcatg ctcggtgatg atctggaaac gatcatcctt tggagccttc aaggtctcga
     4141 ccatggcgtc atagacgacc tctccgatcg tcttgcggta gtccagcggt ttgcccttgg
     4201 cgaggtcgat gcgagcgagc ggcattgggg atctcctttc agatcataag tgctgtgaca
     4261 cggcgcgtca gttcagcggt gggcggtgac cgggtctgtg gcaatatcag gctgccagtt
     4321 gccggctttc ctgtgttcgg ccgtcatacc cccaggcgtt tgccggcact tcggtcacga
     4381 cgacataggt cgcaggatgg agttcggaac caaggacgct tttgagcagt ctcatggtct
     4441 tctcgatgaa gcgcgccttc tcctcggccg aattggtgcc ggcggtgatc gtggcatcga
     4501 catgaaccgc aatcttcgcc agcgtgcccc cgatggccca gccggcactt gccgcctgct
     4561 ccaccagtac actggtgaga tcggcccgct tgccgagaac ggaggccatc aactccgtca
     4621 tctccacttg gagaagcgaa acctggttcg acgcgagcgt cggtcctgag acggtaatgc
     4681 gaataaaagg catggggcgc tccataatct gacggcggcg gtctgttccc ggcaatatgg
     4741 gctcttccca caaagtcgag aaacgaatat atctactggt atggttcaga aaaatagaac
     4801 tgttgtaacg accaacctgg atatcgactt tctccgcacc ctggtgagcg cggaagatct
     4861 cggcgggatg aaccgcgcgg ccgaacaggt cggacggtcg caatcggcag tcagccagca
     4921 aatgcgcaag ctggaagaaa agatcggaca gcgcttgttc cggaagcagg gtcgcggctt
     4981 gatgctcacc gagcccgggg aactgcttct caaatatgcc cgacggatat tggcgctcaa
     5041 cgacgaagcg attgccgctt tgcgcggttc tggtctcgaa ggcgtggtgc ggttcggctt
     5101 gccaagcgac tttgccgaaa cctggctgcc ggcggcgttg ggacagttca agcgggcgca
     5161 cccgctgatc cgcgtcgaag ccagtgtcga ccgcaacacc gtgctgatcg agcgtctgga
     5221 aaaaggcagg ctcgatctag cgatgttctt tagcgatggt cagcatcagg gagcagaacg
     5281 gctggcgacc ctgccgatgg tatggatcgg gagcgcggaa catgcctggg atccgaccga
     5341 gcccattccc ctggtgctgt tcgaggcgcc ctgtgttttc cggaaagcag gtatcgcagc
     5401 gcttgatgcg gcggggattc cctggcggat cgcatttacc agtccaagtc tggcgggtct
     5461 ttgggcggcg gtcgaggcgg gccttgggat aagcccgcga accaccgcgc atttgccgga
     5521 ttggctcaaa gtcctcggca aagcctacaa gcttccgaaa ctgccttcga tcgatctgtc
     5581 gctttacgac ctcggccggc cgctgatgcc agcagccatg cgtctgaagg agattctgac
     5641 cgacaccatt ccgccaggcc tgtccgaatt gcggcgcgcc tcccattcag ggcgtcgtgg
     5701 aggatcggcc gccgcgtgag attcgttgct ctcgcgcgat cgatgaacgc atgcgccagc
     5761 gactgatttg ctttgggcat cagccgatct tcttcaggcc tttcttgaat cgcggcccgt
     5821 tgccgacgta gaatctcgcg gcacttccca tcctggcaac ctgttcggga tcgagggtgc
     5881 ggatcacccg cgccggcgat ccgatgatca gcgaatgctc gggaaactgc ttgccttcgg
     5941 taatgaccga ccctgcgccg acaatgctgc cgcggccgat ccgggcgccg ttcatgacaa
     6001 tcgagcccat cccgaccagc gcgccctcct ccagcgtgca gccgtgcagg atgacattgt
     6061 gaccgacggt gcaattcttt ccgatcgtca gcggaaagcc gaggtcggtg tgacaggtcg
     6121 agttgtcctg cacattggcg ttttcgccga tttcgatcca ctcgttatcg ccgcgcaaca
     6181 ccgcgccgaa ccacacgctc gccgaattgt gcagacggac cttgccgatc accatggcgg
     6241 tgtcggcgat aaagtagttt ccgtccgcag gaagttcggg cgcctgcccg tccagttcgt
     6301 agatcgccat gcctgtctcc tgttcgcgga gacattggca tagccgtatg gatcaggcca
     6361 gcactccggc ggcgcgcagc gtcatcacga aaaacgtgcc ggacatcagg ctccagaacc
     6421 ccgccagcac ggtcgccatc atgacgaatt cgacgcggcg gcgctcgatc cgcgcgccgc
     6481 gcagcgtatt gcgcatgatg atgaagggcg cggcaaaaac caggaacggc accgctgcaa
     6541 acgtcttcgg cgcaaccccg tcttgcagca gcccaaaacc ggcggggcgc cgcaccaagg
     6601 cctgatatcc gctgacgagc gcgccggcca gcgcgaaacc gatgcaaagg gagaacagcg
     6661 aattcaacgc atcaggcgac atcgggaagg tcttccgcaa ttttgaggaa cctgccctgc
     6721 ttcgcaaaat tggccgcccg ccgccacact atccttaagg aagggttaac ggcggcggcc
     6781 ggaaccgcgg ctggcgcatg gcccgcgcaa catcgattcc gtgaaatcgt gcgggaattc
     6841 gatggcccgt ggcatattcg aacgtgattc ggctgcccct tggacgcccc tcacgatgct
     6901 tcgctcatga cattgttttc aaccgcccag caccttgcgc gcggcccgcg cagggatctt
     6961 cgcggccaca tgatggcgat catgctggcg ggcgcggcga ccaccggcgc cattgccctg
     7021 gtcgcctatt tgctgtggcc gacctggggg ccggatcctg caagcagtcc ggcgcggttg
     7081 ccggtcagca tcggtgcgac actttttaac gtgccgaccg ccgccgtccg catgaaaatt
     7141 cagcgtcact ccgggccgca ggaacgcatc gatctcagct tcgcgtttcc ttcgctggag
     7201 gctgccgacg gaccaaggca cgtcagcgcc gataccgtgg cacaagcgat acagccgatc
     7261 gaccggatct tcctgtcgat atcggcgcat cacgacacgc tggcgccgga cctgcgcgtc
     7321 agcaccatct atccgcgcta tcttgagcag gccttgcagc ccggacagga cggattgacg
     7381 atgcgcgcat ttcgggacgg cacgccttac agcaatgaag acctgttcct tgccagcacg
     7441 ccgaccctca gcgcacgctg cacgcgcgac gccgcgaccc cggggatgtg cctgagcgag
     7501 cgccgcatcg acggggccga tctgacgttt cgctttccgc ggagctggct cgcgcaatgg
     7561 cgcgacgtgg ccgatgcggt ggagcgattg acgctgcagt tgcatggggc gacgtgacag
     7621 gcgccgtcat ccttgcgtaa cggcttcgcc gttatcgctg gaggtgcgag ctcccttggg
     7681 cgacttcgaa ggataagatc tgtgcacgtg gcgcatcctt cgagacgccg cgcaaggtgc
     7741 gcggctcctc atcaggatga cgtcagtgcg aggcgggcca aaacctcagc cctgctcgac
     7801 cagatcgtcc tcgaggatcg ccatctggaa ctgaaacgag cggtcgtcgt cttcatcgtc
     7861 gacgaacagc acgccgatga attcctcgcc gatatagact tcagcggaat cgtctttttt
//
LOCUS       DASWGM010000238         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2098486,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000238 DASWGM010000000
VERSION     DASWGM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098486"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1666
                     /locus_tag="VGA15_07265"
     CDS             236..1666
                     /locus_tag="VGA15_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9587530.1"
                     /translation="MIRESPSEPSKEDLIALIAALRAENAALKARLAELERRLELNSS
                     NSGKPPSSDGLKKPARVTSLRERSGKKPGGQKGHKGETLRQVTDPDAVVDHYPPACSM
                     CGAGLDPETSVGHSARQVFDLPEPAPLVVTEHRAHDCQCAACGAKTRALFPDGVNAPV
                     QYGARITAFVIYLLHYQLLPENRLAALMADLFGVKVAAATIARMSRTCAERLRGFAQT
                     VRDLVAGAPVKHMDETGFRIGGKTQWLHVASTALLTFYRVCARRGSLLANVAGIVVHD
                     HWKPYYTMQGVLHALCNAHHLRELKALVDIEKEEWARKMQRLLRRACHAANRARERGV
                     AMKPRLIECFERRYDAILAEGLAFHEAQAPLARAATKGGGKRRGRAPRRTGHNLLLRL
                     ATRKEDTLRFLHDATVPFTNNQAERDGRMMKLRQKISGGFRSSEGAMDFALIRSFFST
                     ARKQGWNIIDALTHHPSNLARSLRLA"
     gene            1732..1807
                     /locus_tag="VGA15_07270"
     tRNA            1732..1807
                     /locus_tag="VGA15_07270"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1764..1766,aa:Asn,seq:gtt)
BASE COUNT          412 a          680 c          646 g          391 t
ORIGIN      
        1 gctcgctgcc atccgtagct tcgatattca gccttctccg cgcgtcctga tcaccggctc
       61 gctctatctc gccggcgagg tgctcgccgc caacggcacg ccgccgagtt aaagtttcaa
      121 ggacgcgaag cccgccctac gaaaattttt ggtaactgcc taggttgggc gaccggaatt
      181 gaattggcga tagatttttc tggacggcgg ctgcagtttt cgattcaaga atcggatgat
      241 tcgcgaatcg ccaagcgagc cgtccaagga agacctgatt gcgctgattg cggcgctgcg
      301 ggccgaaaat gcggctctga aagcgcggct cgccgaactc gagcgccggc tcgaattgaa
      361 cagctcgaac agcggcaagc caccgtcgag cgatgggctg aagaagccgg cgcgggtgac
      421 gagcttgcgc gaacgttccg gcaagaaacc gggcgggcag aagggtcaca aaggcgagac
      481 cctgcgacag gtcactgatc ccgacgccgt cgtcgatcat tacccgccgg cctgctcgat
      541 gtgcggcgcg ggcctcgacc cggaaacgag cgttggccat agcgcgcgac aggtgtttga
      601 tctgcccgag ccggcgcctc tcgtcgtcac cgaacatcgt gcgcacgatt gccaatgcgc
      661 cgcctgcggc gcgaagacgc gcgcgctgtt tccagatggc gtcaacgcgc ccgtgcaata
      721 cggcgccaga atcacggcct tcgtgatcta tctgttacac tatcagttgc tgcccgagaa
      781 ccgcctggcc gcgttgatgg ccgatctctt cggtgtcaag gtggccgccg cgacgatcgc
      841 gagaatgagc cggacttgcg ccgaacgctt gcggggtttt gcccaaacgg tgcgcgatct
      901 ggtggcgggc gcgccggtga aacacatgga cgagacgggg tttcgcatcg gcggcaagac
      961 gcaatggctg catgtggcgt ccaccgcttt gctgaccttc tatcgcgtct gcgccaggcg
     1021 cggcagtctc ttggcgaatg tcgccggcat cgtcgttcac gatcactgga agccctatta
     1081 cacgatgcaa ggcgtgctgc atgccttgtg caatgcccat cacttgcgcg agctcaaggc
     1141 tctcgtcgac atcgagaaag aagagtgggc gcgcaagatg cagcggctgt tgcgccgcgc
     1201 ctgtcacgcc gcgaacagag cgcgagaacg cggcgttgct atgaagcctc ggctcatcga
     1261 atgtttcgag cgtcgttacg acgccattct cgccgaaggg ctggccttcc atgaggccca
     1321 agcgccgctg gcgcgcgccg ccaccaaggg aggcggcaaa cggcgcggcc gcgcgcctcg
     1381 ccgaaccgga cacaatctcc tcttgcgcct cgcgacgcgc aaagaggaca cgctgcgctt
     1441 ccttcacgat gctactgttc ccttcaccaa caaccaagcc gaacgcgacg ggcgcatgat
     1501 gaagctccgt caaaaaatct ccggcggctt tcgctcgtcg gaaggtgcaa tggacttcgc
     1561 cctcatccgc tccttcttct cgaccgcaag gaagcagggc tggaacatca tcgacgctct
     1621 cacccaccac ccatcgaact tggccagatc cctacgtctt gcctgagcca tccagccaac
     1681 ctgggcagta acgaattttt tccgacaact gatcacagtg atggcgcgtt ttccccggta
     1741 gctcagcggc agagcagacg gttgtttccc gtccggtcgg tggttcgaat ccactcccgg
     1801 ggagccacat tccgacattc agattatgaa acgtgggttg gacccgcggc ggaagcggtt
     1861 aaagcaatcg ggctggacca tcgattactg ctgggctacc cccaacgccg gatgaaatcc
     1921 gcaagggtgc gacataacag tcgcgctcgc gcagcaggtg cgcggcatat cgcccagttc
     1981 ggactgacat tggtcggtgg tgaaatatgc aggacgcacg cgtgccggtt ctccgcgcgc
     2041 gcaggattgc ccgcctcgcc gctgcgctcg gggtcgtgat cctcgccgca accgccgcga
     2101 tcaacgcggc cgactatccc aacaagcca
//
LOCUS       DASWGM010000239         3943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1959598,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000239 DASWGM010000000
VERSION     DASWGM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959598"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /gene="otsB"
                     /locus_tag="VGA15_07275"
     CDS             complement(<1..683)
                     /gene="otsB"
                     /locus_tag="VGA15_07275"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX9587531.1"
                     /translation="MNHDLIDKDLAEMPLQEDGPSPEKRPPNAISTSSDFARRLDECA
                     ILLDIDGTLLDLAPTPREVWVPPGLARTLNGLIEKTAGALALVSGRSLNDIDLIFAPE
                     QFPAVGGHGAEMRISGDNESVATHAPPMDKELKRRLAAIAKLSPGILLEDKGYSLALH
                     YRLAPHAEKAIYEAVSLIRADLPNAPIEVLPGKCVCEIKHSGFNKATGVLELMAHEPF
                     RGRRPIFIG"
     gene            complement(690..2138)
                     /locus_tag="VGA15_07280"
     CDS             complement(690..2138)
                     /locus_tag="VGA15_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587532.1"
                     /translation="MAPARGQQDRVIETSIPSRLDSLRWSGFHTRVVLALGITWILDG
                     LEVTLAGALSGALKESPRLHFTNFDVGFSNSAYLAGAVLGALGFGWLTDRIGRKKLFF
                     ITLAVYLTATAATALSWSVASYALFRFLTGAGIGGEYTAINSTIQELVPARYRGWTDL
                     LINGSFWIGAAMGAVSAIVLLDPHLIGPDLGWRLAYLTGACLGLVVFVMRMWIPESPR
                     WLMIHGKPDQAHAIVDDIERSVVGRVQDPRRQAWPKIKLQMRHHTPLREVAHTLFSLY
                     RQRALVGLALMTAQAFFYNAIFFTFALVLTDFFGIASNQVGWYILPFAAGNFLGPLLL
                     GRLFDTLGRRAMITFTYGISGVLLAASGYLFSIGALSAQTQTIAWMVIFFFASPAASS
                     AYLTVSETFPLEVRALAIALFYAVGTGIGGVAGPALFGALIDTGSRNSVFAGYLLGAG
                     LMIGAAIIAWRYCIAAERQPLESVARPLAFVE"
     gene            2463..2702
                     /locus_tag="VGA15_07285"
     CDS             2463..2702
                     /locus_tag="VGA15_07285"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEX9587533.1"
                     /translation="MELIMAEHRKTAPSRKAATLKSPRKNTRKPATRKTSTTRRKASP
                     ELTSYINRAGKTLPKTQRERLQRAKTELKRQFNRE"
     gene            complement(2839..>3943)
                     /locus_tag="VGA15_07290"
     CDS             complement(2839..>3943)
                     /locus_tag="VGA15_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX9587534.1"
                     /translation="AGGFLLGGVLALMLGKGISRPMTAMCKAMRELAGGNFDVVLPGL
                     GRRDELGEMASAVEEFKVQAIAKAERDAAAHDAQNKASSAARRSELIRFADDFEAAVG
                     VIVSNVSASAVQLEQAAGTLTRTAETTQSLSSQVAGASEEASSNMQSVATATKELSTT
                     VDEIGTRVRESNRIAEAAVLQAQQTDGRIGKLSRAAQEIGDVVKLITAIAEQTNLLAL
                     NATIESARAGEAGRGFAVVASEVKSLAGQTAKATDEISSHISGMQDATQESVAAIKEI
                     GGTIAQISSIASSIASAVEQQSSATQEIARSVQSVAQGTREAAASIMQVNRGATETGS
                     ASEDVLNSARSLSTESARLREELDRFMGNIRAA"
BASE COUNT          799 a         1287 c         1162 g          695 t
ORIGIN      
        1 tcgccgataa aaatcggacg gcgccccctg aaaggttcgt gggccatcag ttccagcacg
       61 ccggttgcct tgttaaagcc cgaatgcttg atctcgcaga cgcactttcc gggcagcact
      121 tcgatcggcg cgttcggcag atcggccctg atcagggaaa ccgcctcgta aatcgccttc
      181 tcggcatgcg gggccagccg atagtgaagc gccagcgaat agcccttgtc ttcgagcaga
      241 atgcccggac tgagcttggc aatggccgcc agccgccgct tcaattcctt gtccatcggc
      301 ggggcatggg tggcgacgga ttcattatcc ccggagattc gcatttccgc gccgtggcca
      361 ccaaccgccg gaaattgttc gggcgcaaaa atcagatcga tatcgtttag cgaacgcccg
      421 ctgaccagcg ccagcgcgcc cgcggtcttc tcgatcaagc cgttaagggt tcttgccaga
      481 cccggcggca cccagacctc gcgtggcgtc ggcgccagat cgagcagcgt gccgtcgatg
      541 tcgagcagaa ttgcacactc gtccaaccgg cgcgcaaaat cgctggacgt cgaaatcgcg
      601 ttcggcggcc ttttttccgg tgacggaccg tcttcctgca atggcatctc ggccaaatcc
      661 ttgtctatca agtcgtgatt catttcattc tactccacaa aggccagcgg ccgtgcaacc
      721 gattccagcg gctggcgctc ggctgcgata caatatcgcc acgcgatgat agccgccccg
      781 atcatcaatc ccgccccgag caggtagccg gcgaagacgc tgttgcgcga tccggtgtcg
      841 atcaaggccc cgaacagcgc cgggccggcg acgccgccaa ttcccgttcc caccgcatag
      901 aacagcgcaa tcgcaagcgc gcggacttcc agcggaaagg tctcgctgac ggtgagatac
      961 gcgctacttg cggccggcga ggcaaaaaag aaaatcacca tccaggcgat ggtctgggtc
     1021 tgcgcgctca gcgcgccgat cgaaaacaga tagcccgaag ccgcgagcag aacgcccgaa
     1081 attccgtagg tgaacgtgat catggcgcgg cggcccagcg tatcgaacag ccgacccagc
     1141 agcaaaggcc cgaggaagtt gccggccgcg aacgggagaa tgtaccatcc gacctggttc
     1201 gacgcgatcc cgaagaagtc ggtcaggacc agcgcgaagg tgaagaagat cgcgttgtag
     1261 aaaaacgcct gcgcggtcat caacgccagt ccgaccagcg cgcgctggcg ataaagcgag
     1321 aacagcgtgt gcgccacctc gcgcagcggc gtgtgatggc gcatctgcaa tttgatcttc
     1381 ggccaggctt gccgccgcgg gtcctgcacc cttccgacaa ccgatcgttc gatatcgtcg
     1441 acgatcgcat gcgcctgatc gggcttaccg tgaatcatca gccaccgcgg gctttccggg
     1501 atccacatcc gcatcacaaa caccacgagg ccgaggcagg caccggtgag gtaggccagc
     1561 cgccagccca gatcgggtcc aatcaggtgc ggatccagca acacgatcgc gctcacagcg
     1621 cccatcgccg caccgatcca gaagctgccg ttgatcaaga ggtcggtcca tccgcgataa
     1681 cgcgccggca ccaactcctg tatagtcgaa ttgatcgcgg tatattcgcc gccgatcccc
     1741 gcacccgtca gaaatcgaaa cagcgcatag ctcgccacgc tccaggacag cgcggtggcg
     1801 gccgtggcgg tgagatagac cgccaatgtg atgaagaaca gtttcttgcg tccgatccga
     1861 tcggtaagcc agccgaatcc gagcgcgccg agcacggctc ccgcgagata ggcgctgttg
     1921 gaaaatccga cgtcgaaatt ggtgaaatgc agccgcgggc tttctttcag cgcacctgac
     1981 agtgcgccgg cgagcgtgac ttccagtcca tccagaatcc aggtgatgcc gagggccagc
     2041 acgacacggg tgtggaagcc gctccagcgc agactgtcga gacgcgaggg aatactggtc
     2101 tcgatgacgc gatcttgttg ccctcgtgcc ggcgccattg cgacgtcggt cgaaacaatc
     2161 gtcgcatcgc tcagggcagc catcccgcat tgctccaatg ctcgccgtta tctacacacc
     2221 acagcgactt gacaccacag cgatttggcg ccgcgattca atgccgaact gcgctgcaag
     2281 tccatggacc aaacccaatg aggaaacgcc cggaaatgaa cattcccggg ctctgaggca
     2341 gtaaggccaa aagcgccgcg ccatcgtacc ggccgcatct actaacgcct ccggcaagtt
     2401 cgagttcccg cattccggca aaggaaccga tcgcgtcggc actcgttttc gccagcaacg
     2461 gcatggagtt gatcatggct gagcatcgga agaccgcacc ttcgcgcaag gccgcaaccc
     2521 ttaaaagccc tcgcaagaat acgcgaaagc cggcgacgcg gaagacaagc acgacacgaa
     2581 gaaaggcatc gcccgagctg acgtcttata tcaaccgtgc cggcaagacg ctgccgaaaa
     2641 cccaacgcga acggctacag cgcgccaaga ccgagttgaa gcggcagttt aatcgtgagt
     2701 aatcctcagc gtcgtcccgg cgaacgccgg gacccataac ccccaacgct gattgttaca
     2761 gaaggcctcg atcccagtgc ctaaacgaaa atcctcgacg tatgggtccc ggcgttcgcc
     2821 gggacgacgc aggaggaatc acgccgcgcg gatgttgccc atgaagcggt cgagttcctc
     2881 gcgcaagcgc gcgctttcgg tcgacagcga tcgtgccgaa ttcaacacgt cttcggacgc
     2941 cgagccggtt tccgtggcgc cgcgattcac ctgcatgata ctggcggcag cttcacgggt
     3001 tccctgggcg acgctctgaa cgctgcgcgc gatttcctgg gttgcggagc tttgttgctc
     3061 caccgcgctc gcaatcgacg acgcgatgct cgatatctgg gcgatggtgc cgccaatctc
     3121 cttgatggcg gcaaccgatt cctgtgtcgc gtcctgcatg cccgaaatgt gagacgatat
     3181 ctcgtcggtc gccttcgcgg tctggccggc cagcgatttg acctcggaag cgacgacggc
     3241 gaaacctcga ccggcctcgc cggcacgtgc ggattcaatc gttgcattca gcgccagcag
     3301 attggtctgc tcggcgatcg ccgtgatcag tttgaccacg tcgccgatct cctgcgccgc
     3361 gcgcgacaac ttgccgatgc gcccatcggt ttgctgcgcc tggaggacgg ctgcctcggc
     3421 gatccggttg gattcacgca cccgcgtgcc gatttcatcg acggttgtgg aaagctcctt
     3481 cgtggcggtg gcgaccgatt gcatgttgct ggaggcctct tccgatgcgc ccgcgacctg
     3541 gctggacagg ctctgcgtgg tttccgcggt ccgcgtcaac gtgccggcag cctgttcgag
     3601 ctgaacggcc gatgccgaaa cgttcgagac gatcacgcct accgccgcct cgaaatcgtc
     3661 cgcaaagcga atgagctcgc tgcggcgtgc cgcgctgctc gctttgttct gggcatcatg
     3721 ggccgcggca tcgcgttcgg ctttggcgat ggcctgcacc ttgaactctt ccaccgcgct
     3781 cgccatctcg ccaagttcat ccctgcggcc gaggcccggc agcacgacat cgaaattgcc
     3841 gccggcgagt tcgcgcatgg ccttgcacat cgccgtcatc gggcgggaaa ttcccttgcc
     3901 aagcatcagc gccagcacgc cgccgagcag gaagccgccg gcc
//
LOCUS       DASWGM010000240         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2379071,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000240 DASWGM010000000
VERSION     DASWGM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379071"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1260)
                     /locus_tag="VGA15_07295"
     CDS             complement(13..1260)
                     /locus_tag="VGA15_07295"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9587535.1"
                     /translation="MDEKQLTELTAWLAGAGLAGKSELALVFGFCERAVAAGLPVARA
                     NVLIDTLHPVHEGRIFRWGHDPSLPSEHEYGRTNQIVASGTMEPASGPDAPRADVWRG
                     STFYRMRETGESLLRRRIPAVDETEFNVLPDLRAGGFTDYVAIINRFAADGTIGEMDC
                     VYSSWATKDPDGFQNEHVAALQCIMPFLALAIKSVSLTRMTGTLMQTYLGRDAGRRVL
                     SGRIVRGVADRIDAVLWFSDLRGFTRITDTTPEHVIPLLNDYADVIVSALHEQGGDVL
                     KFMGDGTLAIFPAEDRERACDAALAAAVAARQGVIKLNKRRVADGLPITEMYLGLHVG
                     EVFYGNVGSTERLDFTVIGPAVNEVSRIAAMCRSVEQPMLVSAAFAGVGNNQRRLVSV
                     GRYALRGVGAAQELFTLDPSASR"
     gene            complement(1380..>1655)
                     /locus_tag="VGA15_07300"
     CDS             complement(1380..>1655)
                     /locus_tag="VGA15_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9587536.1"
                     /translation="PHYAASKAGLIGFTHWLAAHLAKQNITANALAPALVESDMVTAD
                     LRARPDRIPIGRFGKPEELAALAVSVLGNGYITGQTINHNGGLYMSS"
BASE COUNT          315 a          524 c          526 g          290 t
ORIGIN      
        1 cgaagccgcc actcaacggg acgcgctcgg atcgagcgtg aagagttcct gagccgcacc
       61 gacgcctcgc agcgcatagc gtccgacgga aacaagccgg cgttggttat ttccaacgcc
      121 cgcgaatgcg gcggacacca gcatcggttg ttccaccgaa cgacacattg ccgcaatgcg
      181 gctgacttcg ttcaccgccg gcccgatcac cgtgaaatcg agccgttccg tgcttccgac
      241 gttgccgtaa aagacctcgc cgacatgaag accaagatac atttcggtaa tcggaaggcc
      301 gtctgctaca cggcgcttgt tcagcttgat gacgccttgc cgcgccgcaa ccgcggcagc
      361 gagcgccgcg tcgcaggcgc gttcccgatc ctcggccggg aaaattgcga gcgtgccgtc
      421 gcccatgaac ttgagaacgt cgccaccctg ctcatgaagc gccgagacaa tgacatcggc
      481 atagtcgttg agcaggggaa tgacatgctc cggcgtcgta tcggtgattc gcgtgaagcc
      541 gcgcagatcg ctgaaccaaa gcacggcatc gatgcggtcg gccacgccgc gcacgatccg
      601 accgctcagc acgcggcgcc cggcatcacg cccgaggtag gtctgcatca gcgttccggt
      661 catgcgtgtg agcgaaacgg atttgatcgc gagggctaga aacggcatga tgcactgcag
      721 ggctgcgaca tgctcgttct ggaacccgtc ggggtccttc gttgcccaag aggaatagac
      781 gcaatccatc tcgccgatgg tgccgtcggc tgcaaagcga ttgatgatgg cgacgtagtc
      841 ggtgaatccc cccgcccgca aatccggcag cacattgaat tcggtttcgt ccaccgccgg
      901 aatgcggcgc cgcaatagcg attcaccggt ttctcgcatc cgatagaacg tactgccgcg
      961 ccagacgtcg gcgcgtggcg cgtccggccc agaagccggc tccattgttc cggatgcgac
     1021 gatctgattg gtgcggccgt actcatgctc ggacggcagg ctggggtcat gtccccatcg
     1081 gaagatacgc ccttcgtgaa ccgggtgcag cgtatcgatg aggacattcg cgcgggcaac
     1141 cggcagtccg gcggcgacgg cgcgttcgca gaacccgaac accaacgcga gttccgactt
     1201 gccagccaag cccgctcccg cgagccaggc ggtaagctcg gtcagctgtt tttcatcgat
     1261 catgcggacg catgatagca gggcccatga tgtggcgcac cgcctggcca ggagaggcga
     1321 acaccgatat cgccaaattc tatcggcgct tgggggcgcg attcgcgcgt ccatgccgat
     1381 caggacgaca tgtacagccc gccgttgtgg ttgatggtct gccccgtgat gtagccgttg
     1441 ccgagcacgg atacggcaag cgcggcgagt tcctccggtt tgccgaaccg gccgatcggg
     1501 atgcggtccg gccgcgcgcg caggtccgcg gtcaccatgt cgctctcgac gagcgccgga
     1561 gcgagcgcat tggcggtgat gttctgcttg gcaagatgcg cggcgagcca gtgcgtgaat
     1621 ccgatgaggc cggccttgga cgccgcataa tgcgg
//
LOCUS       DASWGM010000241         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_434056,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000241 DASWGM010000000
VERSION     DASWGM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434056"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VGA15_07305"
     CDS             <1..250
                     /locus_tag="VGA15_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX9587537.1"
                     /translation="GFDWGGDGARTMTLVYNLPATNAIYQRFAGIDGSAYIIGGFGMT
                     ALTANNIVLVPIRSGLGLRLGANIGYLKYTPRATWNPF"
     gene            374..1675
                     /locus_tag="VGA15_07310"
     CDS             374..1675
                     /locus_tag="VGA15_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587538.1"
                     /translation="MIEPIMYLAIGFLVSMLFGLMIVPLVHNRAVRLTTRRMEAATPL
                     SMAEIQADKDQLRAEFAMSARRLEMNVDQLKNKTTSQLAELGKKSDAINRMKIELGEK
                     NATIFSLEAREKAVKEQLRTTEEEFAAKTEALRTAETVLRDKQSELARFSAELSDRSM
                     TAESREVELVAVRAQIIELRSRVSDAEQEFATTHARLEQQRGESDMATRELAEARSRV
                     ENLSQRVTDLDRQLIIQVKEAEMLGNRVNDLETRLSTQGKLLAERDYENNLLRQSTAT
                     AERTAKELRDEIAAMNGGKAPAIEKLRAEKAAVEEQLRIARDERAKLQRDINAIQAEA
                     ESSWATERMENALLRERINDIAAEVAKLAMQLEGPNSPIEAMLAAEPEMPAKAARPAS
                     GTVANGATANGSGPPDGGGTLAERIRALQYHASRARQQQPT"
     gene            1805..1879
                     /locus_tag="VGA15_07315"
     tRNA            1805..1879
                     /locus_tag="VGA15_07315"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1837..1839,aa:Val,seq:cac)
     gene            complement(2082..2354)
                     /locus_tag="VGA15_07320"
     CDS             complement(2082..2354)
                     /locus_tag="VGA15_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587539.1"
                     /translation="MPGLTSFTEHQVCCQFARENWIDVRGIGLPPVGYDLGGVSGGPM
                     LQPVFVDGAWTWRLVGVVSEALSIEGFERVTAVRSHFILPDGRLSR"
     gene            complement(2540..2824)
                     /locus_tag="VGA15_07325"
     CDS             complement(2540..2824)
                     /locus_tag="VGA15_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587540.1"
                     /translation="MQEFYRRMLDSGSEYVAWFVWRYQSKVMRTPLLPRQGSMFFLDC
                     GRGPFAVTAGHVFERFIKDRAQYRVRGSQIGNIAFNPPGAADRLGQESRA"
     gene            complement(2959..>3288)
                     /locus_tag="VGA15_07330"
     CDS             complement(2959..>3288)
                     /locus_tag="VGA15_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX9587541.1"
                     /translation="WINVTANPTAEWVAHQITEAFPWDRAPRYLIRDRDRIYGAVVTR
                     RLRAMGIRDKPTASASPWQNGFAERLIGSVRRECLDHIIVLGEAHLRRILKSYAHYTT
                     ASERIAL"
BASE COUNT          694 a         1047 c          994 g          553 t
ORIGIN      
        1 cggcttcgac tggggcggcg acggcgcccg caccatgacg ctggtctaca atcttcccgc
       61 gaccaatgcg atctaccagc gcttcgccgg catcgacggc tccgcctaca tcatcggcgg
      121 tttcgggatg accgcgctga ccgcgaacaa catcgtgctg gtgccgatcc gctccggtct
      181 cggcctgcgc ctcggcgcca atattggcta cctcaaatac accccgcgcg cgacctggaa
      241 cccgttctga cttgccccgt tcaggggtta ggtcggccct ggctacgatt cacgttaacg
      301 cggcatcggg ctggcgtttt cgctgcccga tggcattgtg atcagaagag tctctcgcgg
      361 ggagtcctaa tctatgattg agccgatcat gtatctggcg atcggttttc tggtctcgat
      421 gctgttcggg ctgatgattg tgccgctggt gcacaaccgg gcggtgcggc tgaccacccg
      481 gcgcatggag gcggcgacgc cgctgtcgat ggccgaaatc caggccgaca aggatcagct
      541 gcgcgcggaa ttcgcgatgt cggctcgacg gctcgagatg aacgtcgatc agctcaagaa
      601 caagaccacc agccagcttg ccgaactcgg caagaaatcc gatgcgatca accgcatgaa
      661 gatcgagctt ggcgaaaaga acgccacgat cttctcgctc gaagcgcgcg agaaggcggt
      721 caaggaacag ttgcgcacca ccgaagagga attcgccgcc aagacggaag cgctgcgtac
      781 cgccgagacg gtgctcaggg acaagcagag cgagcttgcc aggttcagcg ccgaattgtc
      841 ggaccgttcg atgacggcgg agagccgcga ggtcgagctg gtggcggtgc gcgcccagat
      901 tatcgagctg aggagccgcg tcagcgacgc cgaacaggaa ttcgccacga cgcatgcccg
      961 gctcgaacag cagcgcggcg aatccgacat ggcgacacgc gagcttgccg aggcgcgcag
     1021 ccgggtcgag aacctgagcc agcgcgtcac cgatctcgat cgccagctga tcatccaggt
     1081 caaggaagcc gaaatgctcg gcaaccgcgt caacgatctg gagacgcgcc tttccacgca
     1141 aggcaagctg ttggccgaac gcgactatga aaacaatctc ctgcgccagt cgaccgcgac
     1201 cgcggaacgc accgcaaagg aattgcgcga cgaaatcgcg gccatgaacg gcggcaaggc
     1261 gccggcgatc gaaaagctgc gcgccgaaaa ggccgcggtc gaggaacagt tgcgcatcgc
     1321 gcgcgacgaa cgcgccaagc tgcagcgcga catcaacgcg atccaggcgg aggcggaaag
     1381 ctcatgggcg accgagcgca tggaaaatgc gctgttgcgc gaacgcatca acgacatcgc
     1441 cgccgaagtc gcaaaactcg ccatgcagct cgagggcccg aactccccga tcgaggccat
     1501 gctggccgcg gaaccggaaa tgccggcgaa agccgcccgc cccgccagcg gcaccgtggc
     1561 gaacggcgcc accgccaatg gcagcggtcc ccccgacggc ggcggcaccc tggccgagcg
     1621 catccgggcg ctgcaatacc acgcctcccg cgcccgccag cagcagccga cctgatctct
     1681 ttaggcggtt tgacgctgac agcttgacac cggtaccctg cccttcgtaa atcgcgggct
     1741 ctgataaggt gctttgtccg atcaaacgcc gatttaccgg cctttgatca tcgcacgaaa
     1801 tcccggacgc atagctcagc gggagagcgt tcccttcaca cgggagaggt ccaaggttcg
     1861 atcccttgtg cgtccaccat tacccccttg gattcaatgg cttaacggtt taattccctt
     1921 gcggtgtgcc ccaaaaaagc tggggcacaa tacgggcgca tcggtacggc acggttgcca
     1981 aaaatcgctc cgccgccgcg cccaccctca cccgccgctt tcaggctcag gcaactcggt
     2041 gttgtggtca gaactgcatt ccgacatgaa ttggtgaatg gtcagcgtga aaggcgcccg
     2101 tccggcaaga tgaaatgcga tcggacggcc gtgacacgct cgaagccttc gatgctcagg
     2161 gcttccgata ccacccccac gagccgccaa gtccaagcgc catcgacgaa gaccggttga
     2221 agcatcggcc cgcccgagac accaccgaga tcataaccca ccggcggcaa tccaatcccg
     2281 cgcacatcga tccagttttc gcgcgcgaac tgacagcaga cttggtgttc cgtgaagctg
     2341 gtcaggcccg gcatgggcac tgtgaaggcc gaagaccatc tcatgcggcc cgattgggtc
     2401 gcgctcgcaa cctggaaagc caccgaagaa cacggcttca ttcgggttcg gcggcggagg
     2461 ccaagccgcc tcggtgccct gcacgatttt cttgcccgtc gccgcgatct cctcgggagt
     2521 aacgcggaag gtcgcgatgt caagcccggg attcttgccc caggcgatca gccgctcctg
     2581 ggggattgaa tgcgatattc ccgatctgag agcctcgcac cctgtattga gcgcggtcct
     2641 tgatgaaccg ctcgaacaca tgcccggcgg tgacggcgaa aggcccccgc ccgcaatcga
     2701 ggaagaacat cgagccctgc cggggcagaa ggggcgtccg catcaccttc gactgatagc
     2761 gccacacgaa ccaggcgacg tactcggagc cgctatcgag cattcgccga tagaattcct
     2821 gcattgcaga ggattttcgg tccttgtgtg taccgaaaac ctaagtccga ccatagtggt
     2881 gatgaagtcc gccaaggatg gcgcgtgaac caatgacacc ggtgcgctgt accgaccgag
     2941 agactggagc atccttgttc aaagagcgat gcgttctgac gccgttgtgt aatgggcgta
     3001 agatttcaga atccgacgca gatgcgcttc gcccaggaca atgatgtggt ccaaacactc
     3061 acgccggacc gatccgatca gccgttcggc aaagccattc tgccaaggcg aggcagatgc
     3121 ggtgggcttg tcccggatgc ccatggcgcg cagtcgacgt gtgacgacgg caccatagat
     3181 ccgatcacgg tcccggatca ggtagcgcgg agccctatcc caaggaaatg cctccgttat
     3241 ttgatgtgca acccattctg ccgtcggatt tgctgtgacg ttgatcca
//
LOCUS       DASWGM010000242         6704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_710915,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000242 DASWGM010000000
VERSION     DASWGM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710915"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VGA15_07335"
     CDS             <1..225
                     /locus_tag="VGA15_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX9587542.1"
                     /translation="GLSARALAKALDVPANRLTEIMRGTRDVSADTAIRLGRYFRTDP
                     RFWMNLQAAHDLSKAEKAHSYKKIVPCKAA"
     gene            complement(277..828)
                     /locus_tag="VGA15_07340"
     CDS             complement(277..828)
                     /locus_tag="VGA15_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9587543.1"
                     /translation="MSKPDFQPTLIGPTVIIRPVVAEDWPELFDAGSDPQIWKVHPSP
                     DRYTEPAFRKFFDGAVASKMGFVFVDRATNRLIGSSRYHGYDAERREIEIGWTFIVRE
                     HWGGPTNREVKRLMLDHAFTFVDTVIFWVGERNWRSQGAMTKIGGVRRSGLFTREATG
                     TTPYFIFEIGKDEYEQGGRALVE"
     gene            complement(1022..2173)
                     /locus_tag="VGA15_07345"
     CDS             complement(1022..2173)
                     /locus_tag="VGA15_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX9587544.1"
                     /translation="MTWHFLMTHVTVKDGERALLTRNGRLERVLEPGRHRLFDPRHEL
                     TAELFNVVRAEFPAERYAVLNAARPDLAADLFEVVETKANEIAIVSLDGRPVVLMVPW
                     QVRVYWKVATRVDVERIDVTSDPRVSARHLTMIERTRSTVVMEAVVENHEAGLLYVEG
                     RLVERLAPGRHAFWIVGRKIEVKRLDLRPQAVEITAQEMLTKDRIALRVTLTAFRRIV
                     DPERLVGVVPDVDAWLYRLVQFAIREAVAGRTLDEVLSAKAVLDAELRHYVRERIADS
                     GVEVTELGVKDVILPGEIRELVNKVVEAERVAKANQIRRQEETAATRSLLNTAKLMEE
                     NPLLLRLKELESLERLVEKVGRIDLHAGDGQGFDALLNKLVRLKPTETA"
     gene            complement(2509..2572)
                     /locus_tag="VGA15_07350"
     tRNA            complement(2509..2572)
                     /locus_tag="VGA15_07350"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2542..2544),aa:Met,seq:cat)
     gene            complement(2945..3694)
                     /gene="lepB"
                     /locus_tag="VGA15_07355"
     CDS             complement(2945..3694)
                     /gene="lepB"
                     /locus_tag="VGA15_07355"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9587545.1"
                     /translation="MTSTRATSPRSPWNIVWLGVVGVILLILLVGSPIVFRTFLFQPF
                     NTPSHSMSPTLQQGDHFFVSKYAYGYSHFSLPFSPPLFSGRIFGSEPARGDVVVFRLP
                     KDGSTDYVKRVVGLPGDRIQMKQGLLTINGTPVQRERLADFIGGDSCGSDPAAKVKHW
                     RETLPNGVSHETLDCVDNGFYDNTNVYTVPAGHFFLIGDNRDNSTDSRVLSQVGYIPL
                     ENIIGRAGLIFFSLAPGSGGVRSERIGLFVH"
     gene            complement(3947..5041)
                     /locus_tag="VGA15_07360"
     CDS             complement(3947..5041)
                     /locus_tag="VGA15_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tlde1 domain-containing protein"
                     /protein_id="HEX9587546.1"
                     /translation="MSYRTRAPKNSAARNHRNSSGKTNPYIMFGGTVVGCLVLGCAWI
                     VYANVFGASVYPTATGGNFDVTLVKRAPAPAVPNPPPVFDEVFALTSPAPLISTRVGL
                     RLGPSLTFDDRFAAASPQGEAPKPVPPDRQVAAIAPPQADRQVAQLVRLPSPRPAEAG
                     AAKNSAASARDVAQGAKAAVLAAVEEKPTIFETLFGKLKSAGPSLAFASADASVTGSL
                     GSGNPGQSPNSALGGSVPYDRTTAIYDISKHTVYMPDGTKLEAHSGLGSKLDDPRFVN
                     VRMLGPTPPHVYDLQPRETLFHGVPALRLNPVGGDEAIFGRTGLLAHTYMLGPNGDSN
                     GCVSFKDYNAFLQAYRNGEVKRLAVVTRVE"
     gene            complement(5324..6061)
                     /locus_tag="VGA15_07365"
     CDS             complement(5324..6061)
                     /locus_tag="VGA15_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX9587547.1"
                     /translation="MSSSIPKSQSSSSLKPAAELARASGVRFPNESAEYRRAREALLA
                     EEIELRRHIERVAEQRRALPPGGEVKKNYAFEGEKGPVSFADLFGDKDTLVVYNYMFG
                     PQRERPCPMCTSLLSAWDGEVPDIQQRVALVVVARSPIERLKAFKRERGWHFLPLFSD
                     ISGEFSRDYHALTKDGGDDAAFNVFTRQREPKGATIRHFWGGEMNGASADSGQDPRGA
                     PDLMPIWTILDVTPQGRGTDWYPKLNY"
     gene            complement(6246..>6704)
                     /locus_tag="VGA15_07370"
     CDS             complement(6246..>6704)
                     /locus_tag="VGA15_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9587548.1"
                     /translation="ERENKGLTWPNWPLKMRTSSSQAEGAKREYAVLTQNFSGVDGRV
                     EKLNCVQVDDKFKPIPGTEFELEAQLVLLAMGFVHPVHEGLLKTLGVDLDPRGNVRAN
                     TADYQTSRPNVFSAGDMRRGQSLVVWAIREGRLCARAIDEFLMGTTNLPR"
BASE COUNT         1307 a         2107 c         2034 g         1256 t
ORIGIN      
        1 ggcctgtcgg cgcgagccct ggccaaggcg ctggatgttc cggcgaaccg cctcaccgag
       61 atcatgcgcg gcacccgcga cgtcagcgcg gatacggcaa tccggctggg acgttacttc
      121 cgcaccgatc cgcgcttctg gatgaacctt caggccgcgc acgacctctc caaggcggag
      181 aaggcccaca gctacaagaa aatcgtgccg tgcaaggcgg cctgaccgga ggacgcacga
      241 atgcgagtcc ggtgacagct ccgcgcaacc agcccctcac tccaccaggg cccggccgcc
      301 ctgctcgtat tcgtccttgc cgatttcgaa gatgaagtaa ggcgtcgtgc cggtcgcctc
      361 ccgggtaaac aatccgctgc gtctgacgcc gccgatcttg gtcatcgcgc cctgggagcg
      421 ccagtttctc tcgcccaccc agaagatgac ggtatcgacg aaggtaaatg cgtgatcgag
      481 catcaggcgt ttgacttcac ggttggttgg tccgccccaa tgctcacgga cgatgaacgt
      541 ccagccgatc tcgatctcac ggcgttcggc gtcatagcca tggtagcggc tcgaaccgat
      601 cagccggttg gtcgcgcggt cgacgaacac aaagcccatc ttcgatgcca ccgcgccgtc
      661 gaagaacttc ctgaatgccg gttcggtgta gcgatccgga gaggggtgca ctttccagat
      721 ctggggatcg gagccggcat cgaatagttc gggccaatcc tcggccacca caggccgtat
      781 gatgacggtc ggtccgatca gggttggttg aaaatcaggt tttgacatgc ctagctgata
      841 gcgttgttcg gtcttggggc gcaagcggcc agagccccgt ttcgatggaa tcgaaacgag
      901 gctgattgac gcgctttctt aacgcgaacc cgttgccacc ccgcgatcaa gtccggggcg
      961 ggctttcgct tgaaaacgct ctgctggaaa agtaagggcc gcccgtgggc gacccttctt
     1021 gtcatgccgt ttccgtgggc ttcaggcgaa ccagcttgtt gagcagcgca tcaaagccct
     1081 gcccgtcgcc cgcgtgcagg tcgatccgtc cgaccttctc gaccagccgc tcaagggatt
     1141 ccagttcctt gagccgcagc agcagcgggt tctcttccat cagcttggcg gtgttcagaa
     1201 gcgatcgcgt cgccgcggtc tcttcctgcc ggcggatctg gttcgccttc gcaacccgtt
     1261 ccgcctccac caccttgttc accagctccc ggatctcgcc gggaaggatg acatccttca
     1321 cgccgagctc cgtcacctcg acgccagagt ccgcgatgcg ctcgcgcaca tagtgccgaa
     1381 gctccgcatc gagcaccgcc tttgcggaca gcacctcatc gagcgttcgt cccgccaccg
     1441 cttcgcggat cgcgaactgc accagccggt acagccacgc atccacgtcc ggcacgacgc
     1501 caaccaggcg ttcgggatcg acgatccggc ggaacgccgt cagggtcaca cgcaaggcga
     1561 tgcggtcctt ggtcagcatc tcctgcgcgg tgatctcgac cgcctgcgga cgcagatcga
     1621 ggcgcttcac ctcgatcttg cggccgacga tccagaacgc gtgccgcccg ggcgccagcc
     1681 gctccaccag ccggccctcg acatagagca gaccggcctc gtggttttcg actaccgctt
     1741 ccatcacgac cgtcgatcgc gtgcgctcga tcatcgtgag gtggcgcgcg ctgacgcgag
     1801 gatcgcttgt cacgtcgatg cgctcgacat cgacacgggt cgcgaccttc cagtagacgc
     1861 gcacctgcca gggcaccatg agcaccaccg ggcgtccatc gaggctgacg atggcgattt
     1921 cattcgcctt cgtttccacc acctcgaaca ggtccgccgc aagatcgggc cgtgccgcgt
     1981 tcagcaccgc gtagcgctcg gccgggaatt cggcgcgcac aacgttgaac agctcggccg
     2041 tcaactcgtg ccgcggatcg aacagccggt ggcggccagg ttcgagcacg cgctcaagcc
     2101 tgccgttccg cgtcagaaga gcgcgctcgc catccttcac cgtcacatgc gtcatcagga
     2161 aatgccaggt catggcatgc ctccgtcgtt ggcttttccg gccctgggtc ggaaagcggg
     2221 tttcgtttca aaggatggtg agaacgcgaa cgcggcttcc tgaaagttcg gcgggccgag
     2281 gtttgagctt gcgtcatgac gggcgcgccg gcgcaaccgc ctggcaaagc cgagcgatcg
     2341 cgtggtgctc ccggcatcgg cgcgagcggt atccaccgcg gcacaaagcc ggtcttgcag
     2401 tgtctcaagt tcggacgaaa agccttgcgg cgatacggcc ggaccgcgac tgaagcgcgc
     2461 gtccggtccc cttcagagga atcagctttt caggctgaaa cggagcgctg gagcgggatt
     2521 cgaacccgcg tcaggatgat tatgattcat ctgctctacc actgtgctat ccttcgttgt
     2581 gcaacagacc ggaatcgaac cggtgccttc aggtttaacc ccgacgctct ccatgagcta
     2641 caactgcgtc ttcattcggc ggaacacgaa accgcaaaac cggctcgcgc cagtcgggat
     2701 ctcagagagc ccgaacccat ggcctagcga aggggcgggc taatagacca agcatgttgc
     2761 agttgcaagt ctgcttgtgc gattttgttg agttcgaaag aatgctgtgt cttgcttgtc
     2821 acagcgaacg ccatcgcctg caagcagcgc cgactgacaa tcatcaatcc ttgaagggat
     2881 cgaattcagt ttctcccgcc atcgcaaccg cgaacggaca cagcgtgtgc ggcacgaggc
     2941 tgcctcaatg aacgaacaag ccgatgcgct cggaacgaac gccgcctgag cccggtgcga
     3001 gggaaaagaa gataagcccg gcgcggccga tgatattctc gagcgggatg taacccactt
     3061 gcgaaagtac ccgactatcg gtcgaattat cgcggttatc acctatcaaa aagaaatggc
     3121 cggccggcac ggtgtagacg ttggtgttgt cgtaaaagcc attgtcgacg caatcgagcg
     3181 tctcatgact gacgccgttc gggagggttt cgcgccagtg cttcaccttg gcggccggat
     3241 cggacccgca tgaatcaccg ccgatgaagt ccgccaaccg ctcgcgctgc accggcgtgc
     3301 cgttgatggt gagcagcccc tgcttcattt gaatacgatc gccgggcaat cccaccacgc
     3361 gcttgacgta atcggtcgaa ccatctttcg gcaaacgaaa tacaacgacg tcgccgcgcg
     3421 cgggttcgga gccgaagatg cgtccggaaa acagcggcgg cgaaaacggc agcgaaaagt
     3481 ggctgtagcc ataggcgtat ttggagacaa agaaatgatc gccctgctgc aatgtcgggc
     3541 tcattgaatg gcttggggta ttgaagggct ggaacaggaa agtacgaaaa actattggac
     3601 tccctaccaa caagatcagc agaatcacgc ccacgacgcc aagccataca atattccacg
     3661 gcgatcgcgg gcttgtcgct cgagttgacg tcataaacta ccccaggctg gagttcagcc
     3721 aatttagaca tcatagagtg aagcgcaatc cgcatgtcac gctgctgcgg cgttatcgcg
     3781 ggagatagac gtagcaggcg atcgcggcac agcggttatg gattccgggc tcggcgcttc
     3841 gcgccgcccc ggaatgacga acgacagatt gcgacggctt cgcgatcaac acgaaccaaa
     3901 ttcagcgtgc cgccggtctt gagaaataac cagccagcgc ctccgctcac tccacgcgcg
     3961 tgaccacggc cagccgtttg acctcgccgt tccggtaggc ctgcaggaac gcgttgtagt
     4021 ccttgaacga aacgcagccg ttggaatcgc cgttgggacc gagcatgtag gtgtgcgcga
     4081 gcaggccggt gcgtccgaag atcgcttcgt cgccgccgac gggattgagc cgcagcgccg
     4141 gcacgccatg aaacagcgtc tcgcgcggct gcaggtcata gacatgcggc ggcgtcggac
     4201 ccagcattct gacattgaca aaacgggggt cgtcgagctt ggaaccgaga ccggaatgcg
     4261 cttccagctt ggtgccgtcg ggcatgtaaa ccgtgtgctt ggagatatcg taaatcgcag
     4321 tggttcgatc atacggtacc gatccgccca gcgcgctgtt cgggctctgg ccagggtttc
     4381 ccgatccaag gctgccggtc acgctggcgt ccgcggacgc gaaggccagc gacggtccgg
     4441 ccgacttcaa cttgccaaac agcgtctcga agatcgtggg tttttcttcg accgccgcca
     4501 gcacggccgc cttggcgccc tgcgccacgt cccgggcgga agccgcggaa tttttagccg
     4561 ctccggcctc ggcggggcgt ggcgacggca gccgcaccag ctgtgcgacc tgacgatcag
     4621 cttgcggcgg cgcgattgcg gcgacctggc gatccggcgg caccggcttc ggcgcttcgc
     4681 cctgtggcga tgccgcggca aaccggtcat caaacgtcag cgatggaccc aaccgcagac
     4741 cgacccgcgt ggaaatcagc ggtgcgggtg aggtcagagc gaagacctca tcgaaaaccg
     4801 gtggcgggtt cggaacggcc ggtgctggcg cccgcttgac cagggtgaca tcaaagttgc
     4861 cgccggtcgc ggttggatag acgctggctc cgaaaacatt ggcatacacg atccaggcgc
     4921 agcccagcac gaggcagccg acgaccgtgc cgccgaacat aatatagggg ttggttttcc
     4981 cggaggaatt tctgtggtta cgtgccgcgg aattcttagg cgctcgcgtg cggtaactca
     5041 ttcgccttgc atccagacta gctccacctt tgttcccctt ccaggcagcc ttcgagcgtg
     5101 tgcgtttcac cggcacgttc gaatgcaaat ctgcgcgcaa tgccccgaag aatcagaagt
     5161 cccaaggatg catctcgcag aggcacggag cgtcattaag gcggttttta gttaaatgca
     5221 gattaagtgt gggcggatgt tgggcaggtg gaaacacgga gatcgctccg gcgggagccg
     5281 atctccgtga ttggcccgat ttagagcgtg aaaacgggtg ttttcagtaa ttcagcttcg
     5341 gataccagtc cgtgccccgc ccctgcggcg tgacatccaa aatagtccag atcggcatca
     5401 gatcgggggc gccacgcgga tcctgtccgg aatcggccga agcgccattc atctcgccgc
     5461 cccaaaaatg ccttattgtg gcacctttgg gctcgcgctg ccgcgtaaaa acattgaacg
     5521 ccgcgtcgtc gccgccgtcc ttggtcagcg catgatagtc gcggctgaat tcgcctgaga
     5581 tgtccgagaa cagcggcaga aaatgccagc cgcgctcgcg cttgaaggcc ttgagccgct
     5641 caatcggcga ccgcgcgacc accaccagcg cgacacgctg ctggatgtcc ggaacctcgc
     5701 cgtcccaagc cgatagcagc gacgtgcaca tcgggcacgg ccgctcgcgc tgcgggccga
     5761 acatgtagtt gtagacaacg agggtatcct tgtcgccgaa caggtcagcg aaagagaccg
     5821 gtcccttttc gccctcgaaa gcgtaattct ttttgacttc gccgcccggc ggcagcgctc
     5881 ggcgctgctc ggcgacgcgc tcgatgtgac ggcgcagctc gatctcttca gccagcaacg
     5941 cctcacgggc gcggcgatat tcggcgctct cgttgggaaa acgaacgcca ctggcacgtg
     6001 ccagctcggc ggccggtttc aggctggaac tgctctgaga tttcgggatg gaactgctca
     6061 tggcatgacc tcgcttcggt ggattttggg atcccgaaga taagacgctc cggcagcgga
     6121 cgatcctaca tggtggcagc gataattttc ggccgtcatg cgcggctcac ccgcgtatcc
     6181 atcgatcttc ggaggagtct tgcgaagagg atggattgcc gggtcaagcc cggcaacgac
     6241 gaaagctacc tcggcagatt cgtcgtcccc atcaggaact catcgatggc gcgtgcgcag
     6301 aggcggcctt cgcgaatcgc ccagacgacg agcgactgcc cgcggcgcat gtcgccggcc
     6361 gagaacacgt tcggccgcga ggtctggtag tccgcggtat tggcgcggac attgccgcgc
     6421 gggtcgagat cgacgccgag cgtcttcagc aggccctcat gcaccggatg cacgaatccc
     6481 atcgccagca acaccagttg cgcctcgagt tcgaattcgg tgccggggat cggcttgaac
     6541 ttgtcgtcga cctgcacgca attcagtttt tccaccctgc cgtcgacgcc tgagaagttt
     6601 tgcgtgagca cggcgtattc gcgttttgcg ccttcggcct ggctcgacga ggtccgcatc
     6661 ttcaagggcc agttcggcca ggtcagcccc ttgttctcac gctc
//
LOCUS       DASWGM010000243        10829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1540039,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000243 DASWGM010000000
VERSION     DASWGM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540039"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGA15_07375"
     CDS             <1..182
                     /locus_tag="VGA15_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587549.1"
                     /translation="WHSASRDVILFRIQRAERLGLTYEEYTLEILERGRHLQIEDVER
                     IARIKKARSAFTARS"
     gene            188..703
                     /locus_tag="VGA15_07380"
     CDS             188..703
                     /locus_tag="VGA15_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9587550.1"
                     /translation="MSAIEIKALNASPDLREALSEILVEAVTSGGSVSFMHPLALEVA
                     DTFWEGSLAAAARGERIVLGAFEDESLIGTVTLLLDCPPNQPHRAEIAKMMTRISRRG
                     RGVAKALMRAAESMAVERGRTLLVLDTAADGGASGLYEELGFQLAGVVPDYAFKPHGG
                     LSGTMIYWKRI"
     gene            complement(700..1455)
                     /gene="tpiA"
                     /locus_tag="VGA15_07385"
     CDS             complement(700..1455)
                     /gene="tpiA"
                     /locus_tag="VGA15_07385"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX9587551.1"
                     /translation="MTDAIRPLIAGNWKMNGLKASMAEFEAMLAGAPGVAAKADMLVC
                     PPPTLIAAFAEKARGSKIAVGAQDCHPKASGAHTGDISAEMLGDAGASAIIVGHSERR
                     ADHGESDVLVRQKAEAVWRAGLTAIVCIGETQHQRDAGQTLDICRGQLNVSLPDQARA
                     DNLVVAYEPVWAIGTGLTPTAGDVEQIHGFIRGLLVVRFGGEGAKIRILYGGSVKPSN
                     AAELMAVANVNGALVGGASLKAADFLAIAAGCP"
     gene            1720..3624
                     /locus_tag="VGA15_07390"
     CDS             1720..3624
                     /locus_tag="VGA15_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX9587552.1"
                     /translation="MLRGMRKASSNWLGKAIMSVVMGVLIISFGVWGIADIFRGFGQS
                     TLAKVGHTEISANEFRQLYTEKLQQIGRQFGRPLTMDQARTFGLDRQLLQQTIAEATL
                     DEEARRLGLGQSDAETMRVIFGDPNFKGVGGAFDPARFQAMIRQFGFTEQRYVADQRR
                     VSLRRQIAGTISAGLEPPKVLIEALSRFQNEQRSIEYVKLEAAQAGAIDPPSPETLAG
                     YFDDHKTQFRAPEYREIAFVVINPEEVGKWTTISDEDAKKIFAERRDKLGTPEQRQVS
                     QIVFPNAEEAAAARSRIAAGLSFEDLAKERGLNGSDVDLGMIAKSAVIDPAIADAAFA
                     LPSGDVSQPVQGRFGVALVKIGKIEPGVEPSYESVASDLKKEIASERARKSVAELRDK
                     MEDERGGGASVIEAAKKLGLTAVTVDAVDRSGRLPNGQLVANIPRGLDVVSQAFNSDV
                     GVDNDPIQFNGGYVWYDVLGIKPSRERNLDEVKDQVEARWRDDQIASRLKTKATEMVQ
                     KLDQGGKLADEAANAGLKVETAAGFRRDAALPGVPAAAVAAAFRTAKDGVGQAAGAGT
                     NEWIVFRVTDVTVPPVDLASDDIKKLKETLGRGLTDEQVAQYVTKLETDIGTSINQAV
                     FAQVTGANSN"
     gene            3828..4841
                     /gene="trpD"
                     /locus_tag="VGA15_07395"
     CDS             3828..4841
                     /gene="trpD"
                     /locus_tag="VGA15_07395"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX9587553.1"
                     /translation="MDDLKSIIGKVATGATLSRDEAASAFDSMMSGEATPSQMGGLLM
                     ALRVRGETVDEITGAVTAMRAKMLRVKAPVGAVDVVGTGGDGSGSVNVSTCASFIVAG
                     CGVPVAKHGNRALSSRSGAADVLSSLGVKIDITPPHVGRCIAEAGIGFMFAPSHHPAM
                     KNVGPTRVELATRTIFNLLGPLSNPAGVKRQMVGVFSRQWVQPLAQVLKNLGAESVWV
                     VHGSDGLDEITLTGPTFVAALENGNIRTFDVTPEEAGLKCVGGEALKGGDANANAVAL
                     QSVLDGMPSPFRDVALLNAAAALIVAGRARTLKEGVALGTKSLDSGAAATRLKHLVAV
                     SNG"
     gene            4857..5678
                     /gene="trpC"
                     /locus_tag="VGA15_07400"
     CDS             4857..5678
                     /gene="trpC"
                     /locus_tag="VGA15_07400"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX9587554.1"
                     /translation="MSDILTKIEAYKREEIAAAKRAHPPASVEALAKAATPPRGFVNA
                     IRRKLAHGEYALIAEVKKASPSKGLIRADFDPPVLARAYEAGGAACLSVLTDTPSFQG
                     HLDFMVSARAATALPVLRKDFIYDTYQVVEARAYGADCILIIMAALDDGAAQDIEDAA
                     LAHGMDVLIEIHERAELDRALKLRSPMIGVNNRNLRTFETTLATSEALAPLIPGDRLM
                     VGESGIFAPADLARLARVGMSTFLVGESLMRQADVAAATRALLARGDTVSATGTR"
     gene            5682..6179
                     /gene="moaC"
                     /locus_tag="VGA15_07405"
     CDS             5682..6179
                     /gene="moaC"
                     /locus_tag="VGA15_07405"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX9587555.1"
                     /translation="MARKASKDISALTHIDASGEARMVDVSAKPATERIAVAEGCVVM
                     SKATLELIVSGNAKKGDVLGTARVAGIMAAKRTSDLIPLCHPLALSKVTLDISPDKKL
                     PGCIVRATVKVTGPTGVEMEALTAVSVACLTIYDMIKAVERGVRIEGIHLVEKKGGKS
                     GHYRA"
     gene            complement(6225..6812)
                     /locus_tag="VGA15_07410"
     CDS             complement(6225..6812)
                     /locus_tag="VGA15_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX9587556.1"
                     /translation="MSKQSRKFKVPDELANLYDFANSLDVRHFTHHGVQHVQGDELGS
                     PRELSAWMSRRGLLFTSAKITPAMFETALQLRTSVRSYLQCDPVERRNNAAARSLNQA
                     MGLFPLVAEARSDRMALRGARDDALAGLSLVVAELYDGSVKGILDRLKMCAAEECRRV
                     FFDRSKPATRRWCMSTLCGNRIKTRTYRERHRGVG"
     gene            6918..8165
                     /locus_tag="VGA15_07415"
     CDS             6918..8165
                     /locus_tag="VGA15_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587557.1"
                     /translation="MSREATLPSPRIFYGWLVVAAAFAVTFVGFGCAYTFSAFVEPLQ
                     RDFGASRGSVSLVFSLAGFLYFGLGIVSGPLADRFGSRRLAVAGMVLTGLGLMAASAA
                     RSLTEVYAAYGLGVGLGVGCAYVPAIGAVQRWFVQRRGFASGLAVSGIGVGTLVMPPL
                     ASFLIESLGWRGAYLVLGALAAAVGGGLALLIENDPRDGGLGPDGDPPQLGNQAARSE
                     GASVSEAIRSRRFISLYAACLICSFGVFVPFVHLVPYAQDHGVAPSSAVLLLGVIGVG
                     STAGRFFLGGLADRLGRQFSLLLMFTGMALALAIWVVSANVWSLAAFAFIYGVFYGGW
                     VAVLPTVVMDLFGGRNVSGLIGILYTSVAFGTLIGPSAAGFAFDLSRSYTLPILASAG
                     ANIVAAAIVAATSRVASANKPRG"
     gene            8326..>10829
                     /locus_tag="VGA15_07420"
     CDS             8326..>10829
                     /locus_tag="VGA15_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9587558.1"
                     /translation="MTANGTASINLRHLVRGGPIRWLIVGGVFLIAAITVGATIIAGN
                     FRERALESSERELENTVLLLARHFDQQFQDLGAIQEDFVAYVKSAGIDTSEQYRRRMS
                     SQDIHVMLKAKIGALSYVGGINLFDSDGALINSANAWPVPAVSIADRDFFKTFKSDPR
                     SPSLLVEPVYSRITGLWTTVLARKMTGPNGEFLGTSGRGIEPANFEKFFASLALGDDA
                     AISMYHRNGTLLARHPHVDTMIGMNFSTGPVHQQVLSKSDHGTIRIISPIDGQKRLAS
                     ARILSHFPISVVATTTVAAALADWRQQIRSLIAVAGLSVLAIAALLFLVVHKLSQQHQ
                     LEKQRLDTAINNMTQGLLMFDASRRLVVANQRYIEMFGVSTDVVKPGCTLPQLLQHRK
                     ETGSFAGDVGEYCSNLYGKLAQGKVFQKILDAADGGSIQVSYRPLRRGGWVTTLEDIT
                     ERRRVEERITHLAHYDALTDLPNRALFHEQLKRELARIVPGEQLAVLYIDIDEFKSVN
                     DTLGHLIGDQLLKSVAVSLGHCVRGTDFVARLGGDEFAIVQTAVKTVDDVTDLATRVL
                     DAIRKPVECLGHQVTTDASIGIALAPQHGADLDQILKNADLAMYAAKSAGRRTYRFFE
                     PEMDARVRARRILEMDLRQAIIDGSFEVYYQPSVSLKDNKIVGCEALLRWRHSERGMI
                     SPAEFIPVAEETGLIDELGEWVLTTACAEAATWPDDIKIAVNVSPVQFKSGTLALKIV
                     AALAASGLQASRLELEITEAVLIRDDEAALAILHQLRAIGVRIALDDFGTGYSSLSYL
                     QRFPFDKIKIDRCFVNDIAEPDGSSCIVQAVVNIAAA"
BASE COUNT         1993 a         3396 c         3391 g         2049 t
ORIGIN      
        1 cttggcacag cgcgtcgcgt gacgtgatcc tgtttcgcat tcaaagagcc gaacggctgg
       61 ggctcacata tgaagagtac actttggaaa ttctcgaacg aggccgccat cttcaaatcg
      121 aggatgtcga aagaattgcc cggatcaaga aggcacgctc agccttcacg gctcggagct
      181 agccgtcatg tcggcgatcg aaatcaaggc cttgaacgcc tcgcctgatc tccgcgaggc
      241 gttgagcgaa atccttgtcg aggcggtgac gagtggcggc tcggtcagtt tcatgcaccc
      301 gctggcgcta gaggtggctg acaccttctg ggagggctcg ctcgcggccg ctgcccgggg
      361 cgaacgaatt gtgctcggcg cctttgagga cgagagcctg atcggaacgg tgaccctgct
      421 gctcgactgt ccgccgaacc agccgcaccg ggccgaaatc gcaaaaatga tgacgcggat
      481 cagtcgccgc ggccggggcg tggcgaaggc gttgatgcgc gcggccgaga gcatggccgt
      541 ggagcgcggg cgcacgctcc tggtactcga caccgcggcc gacggcggtg cgtcgggttt
      601 gtacgaggaa ctgggcttcc agttggcggg agtggttccg gattacgcct tcaagccgca
      661 cggcggactc tcgggaacga tgatctattg gaagcgaatc tagggacagc cggcggcaat
      721 cgcaagaaag tcagccgctt tcaggctggc gcccccgacc aaagcgccgt tgacgttcgc
      781 aaccgccatc aattccgcag cattcgaagg tttcaccgaa ccgccgtaga ggatgcggat
      841 ttttgcccct tcgccgccaa accgtacaac cagaagaccc cggataaacc catgaatttg
      901 ctcgacatct ccggcggtcg gggtcaggcc ggtgccgatg gcccagaccg gctcataggc
      961 caccaccaga ttgtcggccc tggcctgatc cggcagcgag acgttgagct gcccgcggca
     1021 gatgtcgagg gtttgcccgg catcgcgctg gtgctgggtt tcgccgatgc atacgatggc
     1081 ggtgagcccg gcgcgccaga cggcttcggc cttttgccga accagcacgt cgctctcgcc
     1141 gtgatcggcg cgccgctcgg agtgaccgac aatgatggcg ctggcgccgg catcgccgag
     1201 catttcggcc gagatatcac cggtatgggc gcccgacgcc ttggggtggc aatcctgtgc
     1261 cccgaccgcg atctttgagc cgcgcgcctt ttcggcgaag gcggcgatca gcgtcggcgg
     1321 cgggcagacc agcatgtcgg ccttggcggc gacaccgggg gcaccggcga gcatggcctc
     1381 gaattcggcc atggaggcct tcaggccgtt cattttccag ttgccggcaa tcagcggccg
     1441 gatggcgtcg gtcatggtgg gattccccgg caaatgttcg gtcaacatgc gccaagcaga
     1501 gccggcgcgc cagttccaga cgccggtttt gcgatccccg agccgttaac cgcttcactt
     1561 cgcctctact tggccctact tgtccctccc cgccaaggtt gcgacgaggc ggctgccact
     1621 ttatgatgct ttatcaactc ggtgatgccc tttcgcggaa ttcgataccg aattccccct
     1681 gggcgcgatc cggttccctc ctgccaacaa gttggaccca tgcttcgagg aatgcggaaa
     1741 gcctcatcta attggctcgg caaggcaatc atgtccgtgg tgatgggcgt gttgatcatc
     1801 agcttcggtg tctggggcat tgccgacatc ttcaggggtt tcggacaatc gacgctggcc
     1861 aaggtcggcc ataccgagat ttcggccaat gaattccgcc agctctacac cgagaagctg
     1921 cagcagatcg gccgtcagtt cggccgcccc ttgacgatgg atcaggcccg cacgttcggc
     1981 ctcgaccgtc agttgctgca gcagaccatc gccgaggcca ctttggatga agaggcgcgc
     2041 cggcttgggc ttggacaatc cgacgccgag accatgcgcg tgatctttgg cgatcccaat
     2101 ttcaagggcg tcggcggcgc attcgatccg gcgcgcttcc aagccatgat ccggcaattc
     2161 ggcttcaccg aacagcgcta tgtcgccgac cagcggcggg tatcgctgcg gcgtcaaatc
     2221 gccggcacga tctcggccgg ccttgagccg ccgaaggtcc tgatcgaggc actcagccgc
     2281 tttcagaacg agcaacgctc catcgagtac gtcaagctgg aggcggcaca ggcgggagcg
     2341 atcgatccgc cgtcgccgga gacgctggcc ggttatttcg acgatcacaa gacccagttc
     2401 cgcgcgcccg aatatcgcga aatagccttt gtcgtgatca atcccgaaga ggtcggcaaa
     2461 tggaccacca tctccgacga ggatgccaag aagatattcg cggaacggcg tgacaagctt
     2521 ggaacgccgg aacagcggca agtatcgcag atcgtcttcc cgaacgccga agaggccgcg
     2581 gcggcgcgta gtcgcatcgc ggccggattg tcattcgagg atctggccaa ggagcgcggg
     2641 ctcaacggtt ccgacgtcga cctcggcatg atcgcaaaat ccgctgttat cgaccccgcg
     2701 attgcggacg cggcctttgc gctgccgtcc ggcgacgtca gccagccggt gcaaggccgg
     2761 ttcggcgttg ctctcgtcaa gatcggcaag atcgagccgg gggtcgaacc ttcctatgaa
     2821 agcgtcgcct ccgatttgaa aaaggaaatc gcctccgagc gcgcgcgcaa atcggtcgcg
     2881 gaactgcgcg acaagatgga agacgagcgc ggcggtggcg ccagtgtcat cgaagccgca
     2941 aaaaaattgg gacttaccgc ggtgaccgtc gacgccgtcg atcgctccgg ccgtctcccg
     3001 aacgggcagt tggtggccaa cattccccgc ggtctcgacg tcgtctcgca ggccttcaac
     3061 agcgatgtgg gtgtcgacaa cgatccgatc cagttcaacg gcggctacgt ctggtacgac
     3121 gttctcggca tcaagccgtc gcgcgaacgc aacctcgatg aagtcaagga tcaggtcgag
     3181 gcaagatggc gcgacgacca gatcgccagc cggcttaaga ccaaggcgac cgagatggtt
     3241 caaaagctcg atcagggcgg caaactcgcg gacgaggcgg cgaacgcagg attgaaagtc
     3301 gaaaccgccg ccggattcag gcgcgatgcc gcccttcccg gcgtgcccgc ggccgcggtc
     3361 gcggcagcct ttcgcaccgc caaggatggc gtgggtcaag ccgccggtgc cgggaccaac
     3421 gaatggatcg tcttccgcgt gaccgacgtc accgtgccgc cggtcgacct tgcttcggac
     3481 gacatcaaga aactgaagga gacccttggg cgcggactga ccgacgaaca ggtcgcgcaa
     3541 tacgtcacca agcttgaaac cgacatcggc acatcaatca accaggccgt ctttgcgcag
     3601 gtgacgggcg cgaatagtaa ctgatgcgtg agtgaaatgg ctcaagagca tgcacggatt
     3661 tccctcaccc tgaggagcgg ctgcgcaggc aagtctacgc agcctgcgta cacttggccg
     3721 ctatccgcgt ctcgaagggt gtgggctctc atggttcgag acggcgcagg tgcgcctcct
     3781 caccaggagg atgtatatct tgcgttcgcc cgacctgaga gcaactgatg gacgatctca
     3841 aatccatcat cggaaaagtt gcgaccggcg ccacactgtc gcgcgacgag gcggcatccg
     3901 ccttcgacag catgatgtcc ggcgaggcca cgccctcgca gatgggcgga ttgctgatgg
     3961 cgctgcgggt gcgcggcgaa accgtcgatg agatcaccgg cgctgtcacc gcgatgcgcg
     4021 ccaagatgct gcgggtcaaa gctccggttg gtgccgtcga tgtggtcggc accggcggcg
     4081 acggctccgg ctcggtcaat gtctcgacct gtgcctcgtt catcgtcgca ggctgcggcg
     4141 tgccggtggc aaaacacggc aatcgcgcgc tgtcgtcgcg ctcgggggct gccgacgtgc
     4201 tgtcctcgct cggggtcaaa atagacatta cgcccccgca tgtcggccgc tgcatcgccg
     4261 aggctggaat cggcttcatg ttcgcgccct cgcaccatcc ggccatgaag aacgtcggac
     4321 cgacccgcgt cgaattggcc acccgcacga tcttcaattt gctcgggccg ctgtccaatc
     4381 cggccggcgt caaacgacaa atggtcgggg tgttttcccg gcaatgggtg cagccgctgg
     4441 cgcaggtcct gaagaacctc ggcgccgaat cggtctgggt ggtacacggc tccgatggcc
     4501 tcgacgagat caccctcacc ggcccgacct tcgtcgcagc cctcgagaac ggcaacatca
     4561 gaacctttga cgtgaccccg gaagaagccg gcctgaagtg tgtcggcggc gaagcgctga
     4621 agggcggcga tgccaatgcc aacgcggtcg ccctgcaaag cgtgctcgat ggaatgccga
     4681 gcccttttcg ggatgttgca ttgctgaacg ccgcggcggc actgatcgtg gccggccgcg
     4741 ccaggactct gaaggagggc gtggcgctcg gcacaaaatc gctcgacagc ggggctgcgg
     4801 caacgcggct aaaacatctg gttgcggtct ccaacggctg agcacgagaa cctgtcatgt
     4861 ccgatatcct gaccaagatc gaggcctaca agcgcgagga gatcgccgcc gccaagcgcg
     4921 cgcatccgcc ggccagcgtc gaagcgctgg cgaaggcggc aaccccgccg cgcggttttg
     4981 tcaacgcgat ccgccgaaag cttgctcacg gtgaatacgc gctgattgcc gaagtgaaga
     5041 aggcctcgcc ctccaaggga ctcattcgcg ccgacttcga cccgcccgtg ctcgcacgcg
     5101 cctacgaagc cggcggtgcg gcgtgtctgt cggtgctgac cgacacgcca tcgtttcagg
     5161 ggcatctcga tttcatggtg tcggcccgcg cggccaccgc cttgccggtg ctgcgcaagg
     5221 attttatcta cgacacctat caggtggtcg aggcgcgcgc ttatggcgcc gactgcatcc
     5281 tgattatcat ggcggcactc gacgacggcg ccgcacagga catcgaggat gcggcgctgg
     5341 cacacggcat ggacgtgctg atcgaaatcc atgaacgcgc ggagctcgat cgggccctga
     5401 aacttcgctc gccgatgatc ggcgtcaaca accgcaatct gcgcaccttc gagaccacgc
     5461 tcgcgaccag cgaggcactg gcgccgctga tccccgggga tcgcctgatg gtcggcgaaa
     5521 gcggcatttt tgctcctgcc gacctcgcgc gacttgcgcg cgtcgggatg tcgaccttcc
     5581 tggtcggcga aagcctgatg cggcaggcgg atgtggcggc ggcgacccgc gcgctgctgg
     5641 cgcgtggcga caccgtcagc gcgacgggga cgcgctagcg catggcgcgc aaagcttcca
     5701 aagacatttc cgccctcacc catatcgacg cctcgggcga agcgcggatg gtcgatgttt
     5761 ccgccaagcc cgcgaccgaa cgcatcgcgg tcgccgaagg ctgcgtcgtc atgagcaagg
     5821 cgacgcttga gctcattgta agcggcaatg ccaaaaaggg cgacgtgctc ggcaccgcgc
     5881 gtgttgccgg catcatggcg gcgaagcgca cctcggatct gatcccgctg tgccatccgc
     5941 tcgctttgtc gaaagtcacg ctggacattt cgcccgataa aaagctgccc ggctgcatcg
     6001 tccgcgccac cgtaaaggtc accggcccga ccggcgtcga gatggaagcg ctgaccgcgg
     6061 tgtcggtcgc gtgcctcacg atctacgaca tgatcaaggc ggtggagcgc ggcgtccgca
     6121 tcgaaggcat tcatctggtg gagaaaaagg gcggcaagtc cgggcactat cgcgcctgag
     6181 gaccaggtta cggcttcgcc ttcacgagcc tcgtgcttcg aggcttagcc gacgccgcga
     6241 tgacgctcgc gataggtccg ggtcttgatc cggttgccgc acagcgttga catgcaccac
     6301 cgccgcgtcg ccggcttcga ccggtcgaag aacacgcgcc ggcactcttc ggccgcgcac
     6361 attttaagac gatccagtat tcccttgacc gaaccgtcgt acaactcggc gaccaccaat
     6421 gacagtccgg ccagggcatc gtcgcgcgcg ccccgcagcg ccatacgatc gctgcgcgct
     6481 tccgccacca gcgggaacag acccatcgcc tggttcaggg atcgagcggc agcgttattg
     6541 cgacgttcaa ccggatcgca ttgcagatag ctgcggacgc tcgttcgaag ctgcaacgcc
     6601 gtctcgaaca tcgccggcgt gattttggcg ctggtgaaca acagcccgcg ccgcgacatc
     6661 cacgcgctga gctcccgcgg gcttcccagt tcatcaccct gcacatgctg cacgccatga
     6721 tgcgtgaaat gcctgacgtc gagcgaattg gcgaaatcgt agagatttgc cagttcgtcc
     6781 ggaaccttga attttctgga ttgtttcgac atttcgacac cagtaaagca tatttgatgg
     6841 ttgacggcaa tcgacactag tatagaattt ttactggtgt ctagcaccgg ccctcacctc
     6901 ttgagacgga ggccgtcatg agccgcgaag caacattgcc cagtccccgt atcttctatg
     6961 gctggttggt ggtggccgcg gcgttcgccg tgactttcgt cggcttcggc tgcgcctata
     7021 cgttctcggc ctttgtcgag cccctgcagc gggattttgg cgcctcgcgc ggatcggtgt
     7081 cgctggtatt ctcgctcgcc ggctttcttt acttcggcct cgggatcgtc agcggtcccc
     7141 tcgccgatcg cttcggctcc cgccgtctcg ccgtcgccgg catggtcctg accggccttg
     7201 gccttatggc cgcaagtgcg gcgcgcagtc tgactgaagt ctacgcggcg tacggattgg
     7261 gcgtcggatt gggcgtcggc tgcgcctatg ttcccgccat cggcgcggtg cagcgatggt
     7321 tcgtgcagcg ccgcggcttt gcctccgggt tggccgtcag tgggatcggc gtcggcacgc
     7381 tggtgatgcc gccgctggca tcgttcttga tcgagagttt gggatggcgc ggcgcttacc
     7441 ttgtgctcgg ggcgctcgct gccgcggtcg gcggaggact ggcgctgttg atcgagaacg
     7501 atccgcgcga cggcggcctc ggccccgacg gcgacccgcc gcaattgggc aaccaggcgg
     7561 cgcgatcgga aggcgcttcc gtgagcgaag cgatccggtc acgccgcttc atcagtctct
     7621 acgccgcctg cttgatctgt tcgttcggcg tgttcgtgcc gttcgttcac ctcgtcccgt
     7681 atgcgcaaga tcacggcgtg gcgccctcat cggccgtgct gctgctgggc gtgatcggcg
     7741 tcggctcgac cgccgggcgg ttcttcctcg gcggccttgc ggacaggctc ggccgccagt
     7801 tttccctgct gttgatgttc acgggcatgg cgctcgcatt ggcaatttgg gtggtttcgg
     7861 cgaacgtctg gtcgctggcc gcctttgcgt tcatctacgg cgtgttctat ggcggatggg
     7921 tcgcggtgct tccgaccgtg gtgatggacc ttttcggcgg ccgcaatgtc agcggcctca
     7981 tcggcatcct ctacaccagc gtggcgttcg gcaccctgat cgggccgagc gcggcgggct
     8041 tcgccttcga cctcagccgc agttacacgc tgccgatcct ggccagtgcg ggcgccaaca
     8101 tcgtcgcggc ggcaatcgtg gcggcgacgt cgagggtggc atctgcgaac aagccgcgcg
     8161 gctaagatag ttgcaacatg cctcgcatag ccgcagccgg atgcctgtta gcccttcatt
     8221 aacctagatt cctaacgcca cctccatttc ggcgcggtac tactgttgcg ggttgggggc
     8281 gcgcgacact gcctctggca ttcaggcctg gcagcaatca agaccatgac ggcaaacggg
     8341 accgcctcca tcaacttgag acatttggtt cgcggcggtc cgatccgctg gctgatcgtt
     8401 ggcggcgtat tcctcattgc ggcaatcacg gtcggcgcga cgatcatagc cggtaatttt
     8461 cgcgagcgcg cgctggaaag cagcgaacgc gagctggaaa acaccgtgct gctgctggcc
     8521 cgtcatttcg accagcaatt ccaggatctg ggggcgattc aagaagactt tgtcgcttac
     8581 gtgaaatcgg ccggaatcga caccagcgag caatacaggc gtcggatgtc cagccaggac
     8641 attcacgtga tgctcaaggc caagatcggt gcattgtcct atgtcggcgg catcaacctg
     8701 ttcgattccg acggagcact gatcaactcg gcgaacgcct ggccggttcc ggccgtcagc
     8761 atcgccgatc gagatttttt caaaaccttc aaatcggacc cccgctcacc atcgcttttg
     8821 gtcgaaccgg tgtacagccg tatcaccggc ctctggacca ccgtcctcgc ccgcaagatg
     8881 accggaccga acggtgagtt cctcggtacc agcggaagag gcatcgagcc ggccaacttc
     8941 gaaaaattct ttgccagcct ggcgcttgga gatgacgcgg cgatttcaat gtaccatcgc
     9001 aacggcacgc tgctggcgcg tcatccgcac gtcgatacga tgatcgggat gaatttcagt
     9061 accggtccgg ttcatcagca agttttgtcg aaatcggatc acggcacgat acgcataatc
     9121 agccccatcg acggccagaa acggctggcc tcggcccgca tattgagcca ttttccgatt
     9181 tcggtcgttg cgacgacgac ggttgcggcg gcgctcgccg attggcggca gcaaatcaga
     9241 tctctgatcg cggtggccgg actttcggtg ctcgcgatcg ccgccctgct gtttctcgtc
     9301 gttcacaagc tgtcgcagca gcatcaattg gaaaagcagc gcctcgacac cgccatcaat
     9361 aatatgacgc aaggcctgct gatgttcgac gcatcgcgcc ggctcgtcgt cgccaaccag
     9421 cgttatatcg agatgttcgg cgtttccacc gatgtggtga agcccggctg caccctgccg
     9481 caactgctcc agcatcggaa ggaaaccgga tcgttcgcag gcgacgtggg cgaatattgc
     9541 tccaatctct acggtaaatt ggcgcaagga aaggtctttc aaaagatttt ggacgccgcg
     9601 gatggcggct cgatccaggt ctcgtaccgg ccgctacgcc gtggtggatg ggtgacaacc
     9661 ctggaggata tcaccgagcg caggcgcgtc gaggagcgga tcacgcacct tgcgcactac
     9721 gatgcgctga ccgacctgcc gaatcgggca ttgttccacg agcagttgaa gcgtgagctg
     9781 gctcgcattg ttcccggcga gcaattggcg gtgctctata tcgacatcga cgaattcaaa
     9841 agcgtcaacg atacgctcgg gcacctgatc ggcgatcaat tgctgaaatc cgtcgccgtg
     9901 agtctcggcc attgcgtcag aggcaccgac ttcgtcgcac gactgggtgg tgacgaattc
     9961 gccatcgtcc agaccgcggt caagactgtc gatgacgtca ccgatctggc gacgcgggtc
    10021 ctcgatgcga tccgcaagcc cgttgaatgt ctcggtcatc aggtaactac cgatgccagc
    10081 atcggcattg cgctggctcc gcagcacggc gccgatctcg atcaaattct gaagaatgcc
    10141 gatctggcga tgtatgccgc caaatccgcc ggacgccgga cttatcgctt cttcgagccc
    10201 gagatggacg cgcgggtaag agcacgccgc atactggaaa tggatctgcg ccaggcgatc
    10261 atcgatggct ccttcgaggt ttactaccag ccgagcgtca gtctcaaaga caacaagatc
    10321 gtcggctgcg aggcgctatt gcgctggcgg cattccgaac gcggcatgat ctctcccgcc
    10381 gagttcattc ccgtcgcgga agagaccggt ctgatcgacg agctcggcga atgggtgctg
    10441 acgaccgcct gcgcggaagc ggcgacgtgg ccggacgata tcaagatcgc ggtcaacgtt
    10501 tcgccggttc agttcaagag cggcacgctg gcgctgaaga tcgtcgccgc actcgcggcg
    10561 tccggtctgc aagcaagcag gctggaactg gagatcaccg aggcggtgct gattcgcgac
    10621 gacgaggcag ccctcgccat tctgcatcag cttcgcgcta tcggcgtcag aatagcactg
    10681 gacgatttcg gcaccggcta ttcctcgctg agttatctgc agcgctttcc gttcgacaag
    10741 atcaagatcg accgctgctt cgtcaacgac atcgcagagc cggacgggtc atcctgcatc
    10801 gtgcaggcgg tcgtcaacat cgccgcggc
//
LOCUS       DASWGM010000244         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_434154,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000244 DASWGM010000000
VERSION     DASWGM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434154"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VGA15_07425"
     CDS             <1..873
                     /locus_tag="VGA15_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9587559.1"
                     /translation="SLVMHPIYAYGDENQRRKYLPKLATGEWVGCFGLTEPDAGSDPG
                     GMKTRAEKTSDGYRLTGTKMWISNAPIADVFVVWAKSAEHNNQIRGFILEKGMKGLSA
                     PKIGGKLSLRASVTGEIVMEGVEVPETALLPNVSGLKGPFGCLNRARYGISWGAMGAA
                     EDCMARARQYTLDRKQFNRPLAATQLVQKKLADMQTDIALGLQASLRVGRLMDEGKMA
                     PEMISIVKRNNCGKALDIARMSRDMHGGNGIQIEYHVMRHAQNLETVNTYEGTHDVHA
                     LILGRAITGIQAFS"
     gene            1003..1926
                     /locus_tag="VGA15_07430"
     CDS             1003..1926
                     /locus_tag="VGA15_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9587560.1"
                     /translation="MADNDDIPFNRDFPLRPGVADEVRPGVRRILCNNPSPFTFTGTV
                     SYIVGKGNVAIIDPGPDDEAHAAALLDAVRNETVTHILVTHTHRDHSPNTARIKAATG
                     AAVYAEGPHRASRPRFESEKHNPESGADRDFRPDVAVKNGDMIEGPGWTLEVVATPGH
                     TANHLAFAWPERRINFVGDHVMGWSTSIVAPPDGSMIDYMASLDRLTARDEDLYFSGH
                     GPEIPDAPRYVRFLIRHRQAREASILHRLAKGETDIPSMVRAIYIGIDPRLTGAAGYS
                     VLAHLEDLVARGIVATDGDPVIGGSYRLAAS"
     gene            complement(2144..2992)
                     /locus_tag="VGA15_07435"
     CDS             complement(2144..2992)
                     /locus_tag="VGA15_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX9587561.1"
                     /translation="MARRFSAPYQSEPVSSLASWARNLAVFSVVAVLVSILIVRFGFL
                     EIKPALATFFGALACAGLSILVGLAAFVAIWQNGSRGMSRILLALLIDAVVLAYPAYL
                     GLQYRKLPPIHDITTDPIDPPRFDALAALRTGDGANTAVYAGLYSAEQQRHYYPDIET
                     VELDVPVQRAYEVALQLVNKRKWLVIDERAPQLPRRIGRIEAVARTPIMGFREDVSIR
                     ATPDGEGSRVDIRSSSRYFESDLGSNAARISRLIDDINNAVDNAKPVVKKPVAPAKVQ
                     PKVVKK"
     gene            complement(3199..>3369)
                     /locus_tag="VGA15_07440"
     CDS             complement(3199..>3369)
                     /locus_tag="VGA15_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX9587562.1"
                     /translation="STRDQARDRYLCAQGYRVMRFWNNDVLSNVEGVLAVIEEGLRQV
                     AESKSGRHDPMV"
BASE COUNT          646 a         1053 c         1087 g          583 t
ORIGIN      
        1 tcgctggtga tgcacccgat ctatgcctat ggcgacgaga accagcgcag gaaatatctg
       61 cccaagctcg ccaccggaga atgggtcggc tgtttcggcc tgaccgagcc ggacgccggt
      121 tccgaccccg gcggcatgaa gacccgggcc gagaagacgt ccgacggcta ccggctgacc
      181 ggcaccaaga tgtggatttc caacgcgccg atcgccgacg tattcgtggt gtgggcgaaa
      241 tccgccgagc acaacaacca gatccgcggt tttattctcg aaaagggcat gaagggcctg
      301 tcggcgccga agatcggcgg caaactctcg cttcgtgcct ccgtcaccgg cgagatcgtg
      361 atggaaggcg tcgaggtgcc ggaaacggcg ttgttgccca acgtgtccgg cctgaaggga
      421 ccgttcggct gcctcaaccg ggcccgttac gggatttcct ggggcgcgat gggcgcagcc
      481 gaagattgca tggcgcgcgc gcggcaatac accctcgacc gcaagcagtt caatcggccg
      541 ttggcggcga cgcaactggt gcagaagaaa ctggcggaca tgcagaccga catcgcgctg
      601 ggcttgcagg cctcgttgcg ggtggggcga ttgatggacg agggcaagat ggcgccggag
      661 atgatttcga tcgtcaagcg caacaattgc ggcaaggcgc tcgacatcgc gcggatgtcc
      721 cgcgacatgc atggcggcaa cggcatccag atcgaatatc acgtgatgcg gcatgcgcag
      781 aacctggaga ccgtgaatac ctatgagggc acccacgacg tccacgccct cattctcggc
      841 cgtgccatca ccggcattca ggcgttttcg tagagtgcgt catgtcgact ccgtaggatg
      901 ggttgagcgc agcgaaaccc atcgtcatag ctccctatgc gttgcgtgat gggtatcgct
      961 gcgctcaacc catcctacga gatcgcaaca tagggtaccc ccatggccga caacgacgac
     1021 atcccgttca accgcgattt tccgcttcgg cccggcgtgg cggatgaagt ccgccccggc
     1081 gtgcggcgga ttctctgcaa caatccgagc cccttcacct tcaccggcac ggtgagttac
     1141 atcgtgggca agggcaacgt cgcgatcatc gaccccggcc ccgacgacga agcccatgcc
     1201 gccgcgctgt tggacgccgt gcgcaatgag acggtgaccc atatcctggt tacccatacc
     1261 catcgcgacc attcgccgaa caccgcacgg atcaaggcgg ccaccggcgc cgccgtctat
     1321 gccgaaggtc cgcatcgcgc gtcgcggccg cgcttcgaga gcgaaaaaca caatccggaa
     1381 tcgggtgccg accgcgactt caggccggac gttgcggtga aaaacggcga catgatcgag
     1441 ggacccgggt ggacgctgga agtggtggcc acgccgggcc acaccgccaa ccatctggcg
     1501 ttcgcctggc cggagcgaag gatcaatttc gtgggcgatc atgtgatggg ctggtcgacg
     1561 tcgatcgtgg cgccgccgga cggatcgatg atcgactaca tggcctcgct cgaccggctg
     1621 acggcgcgcg atgaggatct gtatttctcc ggccacggac cggaaatccc ggacgcgccg
     1681 cgttacgtac gcttcctgat ccggcaccgt caggcccgcg aagcctcgat cctgcatcgc
     1741 ctcgccaagg gcgagacgga tattccctcg atggtgcgcg cgatctatat cggcatcgac
     1801 ccgcggctga cgggtgcggc cggttattcc gtgctggcgc atctggaaga cctggtggcg
     1861 cgtggcatcg ttgcgaccga tggcgacccg gtgatcggcg ggagttacag attggctgcc
     1921 tcttaacctc tccccgttct ttacggggag agcataaggc cgccttcagc ggccgttctc
     1981 ggagaacgcc gaagcggagc ttcggctacg gcgtcttcgc ggcgggtgag gggcaaggca
     2041 caaagctaaa tttgacatcg gcgttcgcgg gaagtgcccc tcaccccaac cctctccccg
     2101 caagtgcggg gcgagggaga ttaccatcgc cccttcccgc tccttacttc ttcacaacct
     2161 tcggctggac cttggccggc gctaccggct tcttcactac cggtttggcg ttgtcgacgg
     2221 cgttgttgat gtcgtcgatc agcctactga tgcgcgcggc gttgctgccg agatcgctct
     2281 cgaaatagcg tgacgaggag cggatatcga cccgcgatcc ctcgccgtcg ggcgtggcgc
     2341 ggatcgacac gtcctcgcgg aagcccatga tcggcgtccg cgccaccgcc tcgatgcggc
     2401 cgatgcggcg cggcagctgc ggggcgcgtt cgtcgatgac cagccatttg cgcttgttga
     2461 ccagctgcag cgcgacctca taggcgcgtt gcacggggac gtcgagttcg accgtctcaa
     2521 tatcgggata gtaatgccgc tgctgttcgg cggaatacaa tccggcatag acggcggtat
     2581 tggcgccgtc gccggtgcgc aaggccgcca gcgcgtcgaa acgcggcgga tcgatcggat
     2641 cggtggtgat gtcgtggatc ggcggcagct tgcgatattg caggccgaga taggcggggt
     2701 aggcgaggac gacggcgtcg atcaacagcg ccagcaggat gcggctcatg ccgcgcgatc
     2761 cgttctgcca gatcgcgaca aaggcggcga gcccaaccag gatcgaaagc ccggcacagg
     2821 ccagcgcgcc gaagaacgtc gccagcgccg gtttgatttc cagaaagccg aagcgaacga
     2881 tgaggatcga gaccagcacc gcgaccaccg agaataccgc gagattgcgc gcccacgacg
     2941 cgaggctgga cacgggctcc gactgatagg gagcggaaaa cctgcgggcc atcgcttcaa
     3001 ttctgccggg ttggaaccgt gccgggcgaa atactgccgg gctgcacact ggcccgtttg
     3061 agaccacgga cctgcggcaa attcaagggg gtggattgcg ggaggggtgt tcttaaacct
     3121 ctccccgctc tgcgcgggga gaggtcggac tgcggagcag tccgggtgag gggcaaggca
     3181 ctgcgcccgc aacgacgatt aaaccatcgg gtcgtgccgg ccagacttac tttcagcaac
     3241 ttgccgcagt ccctcctcga taacagcgag tacaccttcg acgttcgaca gaacatcgtt
     3301 attccagaag cgcatcacac gataaccctg cgcgcacaaa tagcgatctc gcgcctggtc
     3361 gcgtgtcga
//
LOCUS       DASWGM010000245         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1127661,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000245 DASWGM010000000
VERSION     DASWGM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127661"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..264
                     /locus_tag="VGA15_07445"
     CDS             25..264
                     /locus_tag="VGA15_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX9587563.1"
                     /translation="MSEIGERVKKIVVEHLGVEPEKVVDNASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAETILTVGDATKFLEKNAKS"
     gene            362..1627
                     /gene="fabF"
                     /locus_tag="VGA15_07450"
     CDS             362..1627
                     /gene="fabF"
                     /locus_tag="VGA15_07450"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX9587564.1"
                     /translation="MRRVVVTGLGMVSPLGCGVEPTWRRILNSESGARKIDTFDVSDL
                     TSRIACMVPRGDGADGTFNPDQWMEPKDQRKVDDFIIYAMSAAKQALDDANWDPATEE
                     DRCATGTMIGSGIGGLSGIADTALLLKERGPRKVSPFFIPGRLINLASGYVSIEHGLK
                     GPNHAVVTACSTGAHAIGDASRLIALGDADVMVAGGTESPICRLSMAGFCASRALSTA
                     FNDTPEKASRPYDRYRDGFVMGEGAGIVVLEEYEHAKKRGAKIYAEVIGYGLSGDAYH
                     ITSPTPDGDGAFRSMSAAMKRAGITAADIDYINAHGTSTQVGDEIELGAVERLLGNAA
                     SKVSMSSTKSSTGHLLGAAGAVEAIFSILAIRDNIAPPTINLENPSVETAIDLVPQAP
                     RKREIDVALSNSFGFGGTNASVILRRVSN"
     gene            1757..3025
                     /gene="mltG"
                     /locus_tag="VGA15_07455"
     CDS             1757..3025
                     /gene="mltG"
                     /locus_tag="VGA15_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9587565.1"
                     /translation="MSERPPISPRSPRAALEPEQVPPPPKRSERARNPLVVVGNAVIT
                     IVLLLMIGAGAAYVYGKQKIEAPGPLQEDKIVNIPARAGMTDIADILQREGVMDNNRW
                     AFIGSVFALKARSELKPGEYAFQKNASLRDVIGTMVEGKVVQHAVTIPEGLTSEQIVA
                     RLSDNDIFAGAVKEMPREGTLLPETYKFPRGTTRDQVIQRMQQAQKRVLAEIWERRNP
                     DIPIRTPDQLVTLASIVEKETGKADERSRVAAVFVNRLRQRIKLQSDPTIIYGLVGGK
                     GTLGRPIKRSEIQQPSPYNTYVVDGLPPGPIANPGRASLEAAANPARTRDLFFVADGT
                     GGHAFTETYDQHQKNVAKLRTMEKQLQIDAVEPADDGPPPAAAGAPADTNPTATTAKP
                     APAKKPARSSAPAARQGAAQSTASPPVVQQ"
BASE COUNT          644 a          943 c          992 g          540 t
ORIGIN      
        1 tgggaacaag cacgaggttg aacgatgagt gagattggcg agcgggttaa gaagatcgtg
       61 gtcgaacacc ttggtgttga gcccgaaaaa gtggtcgaca acgcgagctt cattgatgac
      121 ctcggcgccg acagcctcga taccgtcgag ctcgtgatgg cgttcgaaga agaattcggt
      181 tgcgaaattc ccgatgacgc cgcggagacg attttgaccg tcggtgacgc cacaaaattt
      241 ctggaaaaga acgcgaaaag ctgacgcccc cgcgcggtgc tgacgaagcc ggccgggccg
      301 tggtcgaacg gtccaccggt ttcttgttgt gggccgcgaa tcgtgggttg gagtggtgga
      361 tatgaggcgg gttgtcgtca cgggcctcgg catggtgtcg cccctcggct gtggcgttga
      421 accgacctgg aggcggattc tcaacagcga gagcggcgcc aggaagatcg acacatttga
      481 cgtctccgat ctgaccagcc ggatcgcctg catggtgccg cgcggcgacg gcgcggacgg
      541 cacgttcaat cccgaccagt ggatggagcc gaaggatcag cgcaaggttg acgatttcat
      601 catctatgca atgagcgcgg cgaagcaggc gctcgacgat gccaattggg atcccgcgac
      661 cgaagaggat cgttgcgcca ccggcaccat gatcggctcc gggatcggcg gcctgtccgg
      721 cattgccgat accgcgctgc ttctcaaaga gcgcggaccg cgcaaggtgt cgccgttctt
      781 cattcccggc cgtctgatca atcttgcttc aggttatgtg tcgatcgagc acggcctcaa
      841 gggtcccaac catgccgtgg tgaccgcgtg ttcgaccggc gcacacgcga tcggcgatgc
      901 gagccggctt atcgcgcttg gcgacgcgga tgtgatggtg gccggcggaa cggaatcccc
      961 gatttgccga ctgtcgatgg cgggattttg cgcgtcgcgc gcgttatcga ccgctttcaa
     1021 cgatactccg gaaaaagcat cgcgccccta cgacaggtat cgcgacggat tcgtgatggg
     1081 ggagggcgcg ggcatcgtgg tgcttgagga atacgagcac gcaaagaagc gtggcgcgaa
     1141 aatctatgcc gaggtgatcg gttatggact gtccggcgac gcctatcata tcacatcccc
     1201 gacgccggac ggcgatggcg cgttccgtag catgagcgcc gccatgaagc gggcagggat
     1261 caccgctgcc gacatcgatt acatcaacgc gcatggaacc tcgacgcagg tcggtgacga
     1321 aatcgagctc ggtgcggtgg agcggctatt gggcaacgcc gcttccaagg tatcgatgtc
     1381 gtcgaccaaa tcgtcgacgg gacatctgct gggcgccgcg ggagcggtgg aggcgatctt
     1441 cagcatcctg gcgattcgcg ataacatcgc cccgcccacc atcaatctgg agaacccatc
     1501 cgttgaaaca gcgatcgatc tggtgccgca agcgccgcgc aaacgcgaga tcgatgtcgc
     1561 gctatccaat tcattcggct tcggaggcac caacgcatcc gtgatcctgc ggcgcgtgtc
     1621 taactgacgc gccgtatcaa gaatactcca ccgtttcgcc gcattcgatg ataaatccta
     1681 gatttgggcg tagactatcg gccagcgcgg gaatcgcggg gccacgatta aaccgacagg
     1741 atttcaggtt gcatcgatga gtgagaggcc gcccatttcg ccccggagcc cgcgcgcggc
     1801 gctggagccc gagcaggttc cgccgccgcc aaagcggtcc gagcgcgccc gcaatccatt
     1861 ggtcgtggtc ggcaatgccg tcatcaccat cgtgctcctc ttgatgatcg gggcgggggc
     1921 cgcatacgtc tacggcaaac agaagatcga ggcccccggc ccgttgcagg aagacaagat
     1981 cgtcaacatt ccggcgcgcg cgggaatgac cgacattgcc gatatcctgc agcgggaagg
     2041 cgtgatggac aacaatcgct gggcgttcat cggaagcgtg tttgcgctga aggcgcgctc
     2101 cgaactcaag cccggcgaat acgcatttca gaagaacgcc agtctgcgcg atgtcatcgg
     2161 caccatggtc gaaggcaagg tagtccagca cgccgtcacg attcccgaag gcttgacctc
     2221 cgaacaaatc gtggcccggc tttcggacaa cgatatcttt gcgggcgcgg tgaaggagat
     2281 gccgcgcgag ggcacgttgc tgccggaaac ctacaaattt ccgcgcggca ccacgcgcga
     2341 ccaggtaatc cagcgcatgc agcaggcgca aaaacgcgtg cttgcggaaa tctgggagcg
     2401 gcgcaatccg gacattccga tcaggacgcc ggaccagctg gttaccttgg cctcgatcgt
     2461 cgagaaggaa actggcaagg ccgacgagcg cagtcgcgtc gccgcggtgt tcgtcaatcg
     2521 gttgcggcaa agaataaagc tgcagtccga tccgaccatc atctatgggc tggtcggcgg
     2581 caagggaacg ctgggccggc ccatcaagcg ctcggaaatc cagcagccgt cgccttacaa
     2641 cacctatgtg gttgacggcc ttccgccggg gccgatcgcc aatcccgggc gggcctcgct
     2701 ggaggccgcc gccaatccgg cgcgcacgcg cgatctgttc ttcgtcgctg acggtaccgg
     2761 cggccatgcc tttacggaga catacgacca gcaccagaag aatgtcgcca aactgcggac
     2821 catggaaaag cagcttcaga tcgatgccgt cgaaccggct gacgatggcc cgccgccggc
     2881 cgcggcgggc gcaccagccg ataccaaccc gacagcgacc acggcgaagc cggcccccgc
     2941 gaagaagcca gctcgcagct ccgcccctgc tgcccgccag ggcgcggcgc aatcgaccgc
     3001 gtcgccgccg gtggttcagc aatagcgcgc acgcggcacc tagcgggaac gaattccagt
     3061 tcgcccgaaa aatgctgcta acttgtaact tgcgcttcta ttcgcaaaac cggtatcca
//
LOCUS       DASWGM010000246         3677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2379406,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000246 DASWGM010000000
VERSION     DASWGM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379406"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VGA15_07460"
     CDS             complement(<1..333)
                     /locus_tag="VGA15_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9587566.1"
                     /translation="MGTSITFKRPDGKDTSGYLANAARGNAPGVVVIQEWWGLSEQIK
                     GLCDRFAVAGFDALAPDLYAGKVVPYHDTDAAGKEMNSLDFIDATTQTVRGAALYLGK
                     NGAKVGLTG"
     gene            complement(611..2680)
                     /locus_tag="VGA15_07465"
     CDS             complement(611..2680)
                     /locus_tag="VGA15_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX9587567.1"
                     /translation="MSGPSDFQPSHPALKWIERRLPIMGLVHSSFVAYPTPRNLNYWW
                     TFGAILSMMLGLQILTGVILAMHYTPEATMAFKSVEHIMRDVNYGWLLRYMHASGASM
                     FFIAVYIHMFRGLYYGSYKEPREILWILGVIIYLLMMATGFMGYVLPWGQMSFWGATV
                     ITNLFSAVPYVGDSIVTLLWGGYSVGNPTLNRFFSLHYLLPFVIAGVVVLHIWALHVA
                     GQNNPAGVEPKTEKDTVPFTPYATIKDAFGLSCFMLFFAWFIFYMPGYLGDADNYIPA
                     NPGVTPAHIVPEWYYLPFYAILRSIPNKLAGVTAMFSAIIVLAFLPWLDSAKTRSSRY
                     RPLAKQFFWIFVIVCVLLGYLGAQPPEGIYVIVGRILTFCYFAYFLILLPLLSRIEKP
                     RPVPNSISDDVLAKTGSKLTPMVSTVIMLAVAGALFAGATQNAWAEEQEVPPSQKWSF
                     AGPVGKFDRGSLQRGLKVYKEVCAVCHGLSYIAFRNLADAGGPGYSEAQAAAFAADYK
                     IKDGPNDQGEMFERPGRPADYFPSPFPNEQAARAAMGGAFPPDLSLINKARSYERGFP
                     WFILDFFTQFQEQGPNYVDAILQGFEDKPPAGVSIPEGSYYNKYFPGHAIKMPKPLSD
                     GQVTFDDGSPATVAQYAKDVTTFLAWTAEPHMEARKRLGLQVFVFLIIFAGLMYFTKK
                     KVWADAH"
     gene            complement(2744..3271)
                     /gene="petA"
                     /locus_tag="VGA15_07470"
                     /gene_synonym="fbcF"
     CDS             complement(2744..3271)
                     /gene="petA"
                     /locus_tag="VGA15_07470"
                     /gene_synonym="fbcF"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX9587568.1"
                     /translation="MTTASSMDSTRRDFLFVATGAAAAVGGLATLWPFVSQMNPDAST
                     IAAGAPIEVDLAPIAEGQDIKVFWRGKPIYISHRTKKQIDEARAVPVASLPDPQADQA
                     RVKEGHDQWLVVIGICTHLGCIPIAHEGNYDGFFCPCHGSQYDSSGRVRQGPAPANLP
                     VPPYQFVSDSKIQIG"
     gene            3574..>3677
                     /locus_tag="VGA15_07475"
     CDS             3574..>3677
                     /locus_tag="VGA15_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA methyltransferase"
                     /protein_id="HEX9587569.1"
                     /translation="MQIALFQPDIPQNTGTILRLCACLNVAAHIIEPAG"
BASE COUNT          753 a         1101 c         1176 g          647 t
ORIGIN      
        1 gccggtcagg ccgaccttgg cgccgttctt gccaaggtaa agtgctgcac cgcgcacggt
       61 ctgggtggtg gcgtcgatga aatccagcga gttcatctcc ttgccggccg catccgtgtc
      121 gtgatacggc accaccttgc cggcatagag atcgggcgcc agggcgtcga agcccgccac
      181 cgcgaagcgg tcgcacagcc ccttgatctg ctccgaaagc ccccaccatt cctggatcac
      241 gaccacgcca ggcgcgttgc cgcgcgcggc gttggcgaga tagccgctgg tgtccttgcc
      301 gtccggacgc ttgaacgtga tgctggtacc catgggttcc tccgatggtt ttctagaggg
      361 ccgattggcg ggtattttgt ccgcagccgg gccaggaagc aatcggttgc ttcctaacct
      421 cgccccgctc tgcgcgggga gaggtcggcg cgcagcgccg ggtgaggggc aaggcactgc
      481 gctcattgtg agctcggaat tagcaaaggc tgcaatcttg gcagccgctg tgcctcgccc
      541 ctcaccccaa ccctctaaga gccagcttcg ctcgtctcga ccccgcaagt gcggggcgag
      601 ggagaagaga tcaatgtgcg tcggcccaga ccttcttctt ggtaaaatac atcagccccg
      661 cgaatatgat caggaacacg aacacctgca ggccgaggcg cttgcgggcc tccatatgcg
      721 gttccgcggt ccacgccagg aaggtggtga catccttggc gtattgcgcg acggtagccg
      781 gcgagccatc gtcgaacgtg acctggccat cgctcagcgg cttcggcatc ttgatggcat
      841 ggccggggaa atatttgttg tagtaggagc cctcgggaat gctgacgccc gccggcggct
      901 tgtcctcaaa accctgcagg atcgcatcga cgtaattcgg gccctgctcc tggaattgcg
      961 tgaagaagtc caagatgaac caggggaagc cgcgctcata ggaacgcgcc ttgttgatca
     1021 gggacaggtc aggcggaaat gcgccgccca tggctgcgcg cgcggcctgc tcgttgggga
     1081 acggcgaggg gaagtaatcg gcgggccggc cgggccgctc gaacatttcg ccctgatcgt
     1141 tcgggccatc cttgatcttg tagtcggcgg cgaaggctgc cgcttgggct tccgaatatc
     1201 cggggccgcc ggcatcggcg agattgcgga acgcgatata ggacaggccg tgacagaccg
     1261 cgcagacttc cttgtagacc ttcaagcccc gctgcaacga cccgcgatcg aacttgccga
     1321 ccgggccggc gaacgaccat ttctgcgacg gcggtacttc ctgctcctcg gcccaggcgt
     1381 tctgcgtagc tccggcgaac agggcgccgg cgaccgcgag catgatcacc gtcgacacca
     1441 tcggcgtcag cttgctgccg gttttcgcca gcacgtcgtc ggagatcgaa ttcggaaccg
     1501 gcctcggctt ttcgatccgg ctcagcagcg gcagcaggat caggaaatag gcaaagtagc
     1561 agaaggtcag gatgcgcccg acgatcacat agatgccttc gggcggttgc gcgccgagat
     1621 agcccaagag cacgcagacg atgacgaaga tccagaagaa ctgcttggcc agcggacgat
     1681 atctggacga cctcgtcttc gcgctatcga gccatggcag gaacgccagc acgatgatcg
     1741 ccgagaacat cgcggtcacg ccggcgagct tgttcgggat cgagcgcagg atcgcgtaga
     1801 acggcagata gtaccactcc ggcacgatat gcgcaggcgt cacgccggga ttggcgggga
     1861 tgtagttgtc ggcgtcgccg agatagcccg gcatgtagaa gatgaaccag gcgaagaaca
     1921 gcatgaagca ggaaaggccg aacgcatcct tgatggtcgc gtagggtgtg aacggcacgg
     1981 tatccttttc cgttttcggc tcgacgccgg ccggattgtt ctgaccggcg acatgcagcg
     2041 cccagatgtg cagcaccacc acgccggcga tcacgaatgg caatagataa tgcagcgaga
     2101 agaagcggtt cagcgtcgga ttgccgaccg aatagccgcc ccacagcagg gtcacgatgc
     2161 tgtcgccgac atagggaacc gcggagaaca gattggtgat gacggtggca ccccagaagc
     2221 tcatctggcc ccacggcagc acgtagccca tgaagcccgt cgccatcatc aggaggtaga
     2281 tgatgacgcc tagaatccag aggatttcgc gcggctcctt gtatgatccg taatacaggc
     2341 cgcggaacat gtggatgtag acggcgataa agaacatcga ggcgccgctg gcgtgcatat
     2401 agcgcaacag ccagccgtag ttgacgtcgc gcatgatatg ctcgacggat ttgaaggcca
     2461 tggtggcctc cggcgtgtag tgcatcgcca ggatcacgcc ggtcaggatc tgcaagccga
     2521 gcatcatcga gaggatggcg ccgaaggtcc accagtagtt cagattgcgc ggcgtcggat
     2581 aggccacgaa cgaagaatgg acgagaccca taatcgggag gcgccgctca atccacttca
     2641 aggcggggtg ggacggctga aagtcggatg gtccgctcat gatgcgatcc tgaggaagta
     2701 aaacgacgcg atggctccaa gtctcggata tctggtccga agctcagccg atctggattt
     2761 tgctatcgga aacgaattga tagggcggca ccggcagatt ggccggcgct ggcccctggc
     2821 gaacgcgacc cgaggagtcg tattgcgagc cgtggcacgg acagaagaag ccgtcgtaat
     2881 tgccttcatg ggcgatcgga atgcagccga gatgggtgca gatgccgatc acgacgagcc
     2941 actggtcgtg cccctccttg acccgggcct ggtcggcctg cggatcgggc aggcttgcga
     3001 ccggcaccgc gcgggcctcg tcgatctgct tcttggtgcg gtggctgatg tagatcggct
     3061 tgccccgcca gaacaccttg atgtcctgcc cttcggcaat cggcgccaga tcaacttcga
     3121 tcggcgcgcc cgccgcaatc gtcgaggcgt ccggattcat ttgggagacg aaaggccaga
     3181 gggtagccag tccgccgacc gcagcggcgg cgcccgttgc aacaaaaagg aaatcacggc
     3241 gtgttgaatc catcgaagac gctgtcgtca cgattccaaa cctttctcaa gttgcagccg
     3301 gtggaaccgc cccaggcaac gcatcaacga aaccggagca ggctgccggc gcccgcgacc
     3361 cccgcggcgg cagaaagacc acttttcccg gccaatcggg gcggaacaag cagtccagaa
     3421 tcgttctatt ggcacccttg ccggaagagc gcaagcccgc tatcggctcg catgcgtgca
     3481 atgcactcat tccgcgcgga gtggtgattt attgagacat ttcatgggga gaccccagga
     3541 acgatcagcg gacgatcagc ggacgaccag cgaatgcaga ttgcactttt ccagcccgac
     3601 atcccgcaga ataccgggac gatcctgcgt ctgtgcgcgt gcctgaatgt ggccgcccat
     3661 atcatcgagc cggccgg
//
LOCUS       DASWGM010000247         3047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2379414,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000247 DASWGM010000000
VERSION     DASWGM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379414"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VGA15_07480"
     CDS             complement(<1..504)
                     /locus_tag="VGA15_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9587570.1"
                     /translation="MSRIAPLEPPYAPEIGEQFDRIMRGAPPLMLFRVIAGNARAWEK
                     FRAGSLLDRGPLSLREREIVIDRTCARTGCEYEWGVHIAAFAAAAHLTDAQVRATVLE
                     GADAPCWSAAEQALIAAVDALHLRASLGDDEFKALSAHYDDAKIFEIILLCGFYRTVS
                     YLANGLDL"
     gene            577..918
                     /locus_tag="VGA15_07485"
     CDS             577..918
                     /locus_tag="VGA15_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9587571.1"
                     /translation="MLKQISAVKKRAVRGSRSGRPIMALLDLLGRRWTLRIIWELREQ
                     PLTSRALRAACDEASPTVLQARLSDLRHAGLVELMPASGYRLTAMGRELHETFLPLHR
                     FAERWSKLKAD"
     gene            complement(922..1368)
                     /locus_tag="VGA15_07490"
     CDS             complement(922..1368)
                     /locus_tag="VGA15_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX9587572.1"
                     /translation="MTEMFSTLGTWNWLIFGLILMTLELLAPGVFLFWLGLAALLVGL
                     ISFAINPSWQLQMLMFAVFAAAAVLLWRRVARNNTAVSKSNRFLNKRAEALVGRVFTL
                     EKPIIDGWGTVRIDDTIWRVAGPDTPAGSRVRIVRADGASLTVAPA"
     gene            complement(1380..2381)
                     /locus_tag="VGA15_07495"
     CDS             complement(1380..2381)
                     /locus_tag="VGA15_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9587573.1"
                     /translation="MTGFDIFAIALVLLVCFTLYAGVKTVPQGYDWTIERFGKFTRTM
                     SPGLNIIIPYFDRVGRKMNMMEQVISIPEQEVITKDNATVTVDGVAFYQVFDAAKASY
                     EVSNLNQAIIVLTMTNIRSVMGAMDLDQVLSHRDEINERLLRVVDAAVSPWGLKVNRI
                     EIKDIVPLADLVEAMGRQMKAERVKRADILQAEGQRQSEILRAEGAKQGQILQAEGRR
                     EAAFRDAEARERSAEAEAKATQMVSEAIAKGDVAALNYFIANNYIKAFGQFADSPNQK
                     VIMLPIEAVSLLGSLAGIGEIAKAAFGESAESAQAAARRPSVPSAGPNPPPPTTPQP"
     gene            complement(2585..>3047)
                     /gene="hemH"
                     /locus_tag="VGA15_07500"
     CDS             complement(2585..>3047)
                     /gene="hemH"
                     /locus_tag="VGA15_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX9587574.1"
                     /translation="AVSINTHLKTLPFQPELIVASFHGMPQKYVDKGDPYYAQCVATT
                     DALRKRMGLDASKLILTFQSRFGFDAWLRPYTDQTMERLAKEGVRRIAVVMPGFSADC
                     LETLEEIAQENAEIFKHHGGEQFAAVPCLNDSDPGMDVIRQLVLRELQGWI"
BASE COUNT          569 a          982 c          933 g          563 t
ORIGIN      
        1 caaatccagc ccgttcgcca gatacgacac ggtgcgatag aagccgcaca gcaggatgat
       61 ttcaaagatc ttcgcgtcgt cgtaatgcgc cgacagcgcc ttgaattcat cgtcgccgag
      121 gctggcgcgc agatgcagcg catcgaccgc cgcgatcagc gcctgctcgg cggccgacca
      181 gcacggcgcg tcggcgccct ccagcaccgt ggcgcggacc tgcgcatcgg tgagatgcgc
      241 cgccgcggca aaggctgcga tatgcacacc ccattcatac tcgcaaccgg tgcgcgcgca
      301 ggtgcggtcg atgacgattt cgcgttcccg caacgacagc gggccgcggt cgagcagact
      361 gccggcgcgg aatttctccc aggcccgcgc gttgccggca atgacgcgga acagcatcag
      421 cggcggcgcg ccgcgcatga tccggtcgaa ttgctcacct atctccggtg cataaggcgg
      481 ctcaagcggt gcgatacgtg acatggccaa ctcccgtgct acaataaacg tagcgtcatg
      541 ctacaatata tgtagcaaac tgcaagaagg acttcgatgc tgaaacagat ttcagccgtg
      601 aaaaaacgcg ccgtacgcgg ctcccgctcg ggccgcccga tcatggcgct gctcgacctg
      661 ctgggccggc gctggacctt gcggatcatc tgggagcttc gcgagcagcc gctgacgtcg
      721 cgcgcccttc gcgccgcctg cgatgaagct tccccaaccg tgctgcaggc ccggctgtcg
      781 gatctgcggc acgccggcct ggtcgaactc atgcccgcaa gcggatatcg cctgaccgcg
      841 atgggccgtg aattgcacga aaccttcctg ccgctgcacc gctttgccga gcgatggagc
      901 aagctcaagg ccgattgatc ttcaggccgg cgccaccgtc aggctggcgc catcggcccg
      961 cacgattctg acgcggctgc cggcgggggt gtcggggccg gcgacgcgcc agatcgtatc
     1021 gtcgattctt accgtgcccc acccgtcgat gatcggcttt tccagcgtga acacccggcc
     1081 gaccagcgct tcggcgcgct tgttgagaaa ccgattgctc ttgctgacgg cggtattgtt
     1141 acgcgcaacc cgccgccaca gcagcaccgc agccgcggca aaaaccgcga acatcagcat
     1201 ctgcaactgc caggacggat tgatcgcgaa cgatatcaac ccgaccagca acgcggcgag
     1261 cccgagccag aacaggaaga ccccgggcgc gagcagctcc agcgtcatca ggatgaggcc
     1321 gaagatcagc cagttccagg tgcccagcgt ggaaaacatc tcggtcatga tatgacccct
     1381 cagggttgcg gcgtcgtcgg cggcggcggg ttcggtccgg cgctcggcac cgacggacgg
     1441 cgcgcggcgg cttgcgcgga ttccgcactt tcaccgaagg cggccttggc gatctcgccg
     1501 ataccggcga gcgagcccag caggctcacc gcctcgatcg gcagcatgat caccttctgg
     1561 ttcggcgaat ccgcaaattg cccgaacgcc ttgatgtaat tgttggcaat aaaataattc
     1621 aacgcggcga catcgccctt ggcgatggct tcactgacca tctgcgtcgc cttggcctcg
     1681 gcttccgcgg aacgttcgcg cgcttcggca tcgcggaacg cggcttcgcg gcggccctcg
     1741 gcctgcagga tctggccctg tttggcgccc tcggcccgca ggatttccga ttgccgctgg
     1801 ccctcggcct gcagaatgtc ggcgcgcttg acgcgctcgg ccttcatctg ccgtcccatc
     1861 gcctcgacga ggtcggcgag cggcacgata tccttgatct cgatgcggtt gactttcaat
     1921 ccccacggcg acaccgcggc atcgaccacg cgcagcaatc gctcgttgat ctcgtcgcga
     1981 tgcgacagca cctggtcgag atccatcgct cccatcaccg agcggatgtt ggtcatggtc
     2041 aaaacgatga tcgcctggtt gagattggag acctcgtagc tcgccttcgc ggcatcgaac
     2101 acctggtaga aggcgacgcc gtctaccgtc acagtggcgt tgtccttggt gatcacctcc
     2161 tgctcgggga tgctgatcac ctgctccatc atgttcatct tgcggccgac gcggtcgaaa
     2221 taggggatga tgatgttcag ccccggtgac atcgtgcggg tgaatttgcc gaaccgctcg
     2281 atggtccagt catagccttg cggcaccgtc ttgacgccgg catacagcgt gaaacaaacc
     2341 agcagcacaa gcgcgatggc gaagatgtca aaaccggtca taactcctcc aaagccgggc
     2401 gcgacgcgct cccgagcgcc gtcttgtttt gtctgcaagc cggccgcgac ggttcagccg
     2461 gctgttgttg aatataggcg agtcttgaat ataggtaggc cttgaacata ggtaggtcct
     2521 ggtttgccgc ccgccagatc catggcaagc gaacgccggc gatttgtatg gcgtcactga
     2581 accgtcaaat ccagccctga agctcgcgca gcaccagttg gcggatgacg tccatgccgg
     2641 ggtcgctgtc gttgaggcag ggaacagcgg cgaattgctc gccgccatga tgcttgaaga
     2701 tctcggcgtt ttcctgcgcg atttcctcca gcgtttccag acaatcggcg gaaaagccgg
     2761 gcatcaccac ggcgatacgg cggacgccct ccttcgccag cctctccatg gtctggtcgg
     2821 tatagggccg cagccacgcg tcgaagccga agcgcgactg gaaggtgagg atcagttttg
     2881 aggcatcgag gcccatgcgc ttgcgtaacg cgtccgtggt cgcgacgcat tgcgcgtaat
     2941 agggatcgcc cttgtcgaca tatttctgcg gcatgccgtg gaacgaggcc acgatcagct
     3001 cgggctgaaa cggcaaggtt ttgagatgcg tattgatcga gaccgcc
//
LOCUS       DASWGM010000248         4513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_986460,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000248 DASWGM010000000
VERSION     DASWGM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986460"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2149)
                     /locus_tag="VGA15_07505"
     CDS             complement(<1..2149)
                     /locus_tag="VGA15_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX9587575.1"
                     /translation="MIARLIDWSARNLMLVLIGAVFAAAAGLYALQHSALDAIPDLSD
                     TQVIVYTEYQGQAPQVIEDQVTYPLTTAMLTVPKSKVVRGFSFFGVSFVYIIFEDGTD
                     IYWARSRVLEYLNAAARRLPNGVTPTLGPDATGVGWVYQYALQSKDKSLADLRSLQDW
                     TIRYGIAKAEGVAEVASVGGFVRQYNIILDPNRMRSLGIPLAKVRDAVRGSNMDVGGR
                     SVELSEFEFIVRGRGYVKSLSDLSNIVVKVDKGTPVLLKDVARIEFGPDERRGITELN
                     GEGEVASGIALQRFGQNALDVIDNVKARLADMASALPKGVEIIPVYDRSNLIHNAIET
                     LKRTLTEESIVVALVCIAFLLHVRSALVAIVMLPVGILMAFAAMKALGIGSNIMSLGG
                     IAIAIGAMIDGAIVMIENAHKHLERAPPDKPRLEVLIEAASQVGPALFFSLLIITVSF
                     LPIFTMESQEGRLFSPLAFTKTFSMAAAAVLSITLVPALMVLFIRGKIIPEHKNPINR
                     FLIWVYRPLIQGVLKAKTLTIFLALVALAVSLWPARQLGTEFMPSLNEGTLMYMPTTL
                     PGLSVTKAAEILQTQNKVIKTFPEVESVYGKAGRAETATDPAPTEMFETIVNLKPKAQ
                     WRAGLTVDGLIAELDKALQFPGVSNAWTMPIKARTDMLATGIRTPIGIKVLGKDLTEM
                     EKLARQIETVVKAVPGTSSAFAERVIGGYYLDVV"
     gene            complement(2146..3579)
                     /locus_tag="VGA15_07510"
     CDS             complement(2146..3579)
                     /locus_tag="VGA15_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9587576.1"
                     /translation="MRRPVGAFLIVSAILAAAVVGLAVGRSNGPLPGWLDGVLPVAAK
                     QSMAQSEASGPIIYYRDPDGLPDYSLTPKKTSSGKDYSAVRASEDVSFEEKAPETMTA
                     KGGDRGKIRFYRNPMGLPDTSPTPKKDSMGMDYLPVYEGEQDDDSSVKVSVGKLQKAG
                     VQTEVAERRTLNTVVRAPGTVQEDERRRSVVSLRFEGFIDSVENVTTGTHVHKGQPLM
                     RVYGPNLSSAAAEYLSALNARPDAGISNQALKGARRRLENLDAPEKFIADIERTREIP
                     VYASWPAPQDGEIVERAAVNGMRAGPGDVLFRIADHQVVWVLVDVAERDLSLVEVGQK
                     AGVRLRAYPDRVFNGKVALIYPHLMAETRTARVRIELPNPDEVLRPDMYADVEIATGT
                     EAPVVTVSSSAVIDSGERQIVLLDKGDGRFEPRAVKLGRRGDGRIEIKEGLAENDKVV
                     VSANFLIDAESNLKAALSGLDSGEKSQ"
     gene            complement(3737..>4513)
                     /locus_tag="VGA15_07515"
     CDS             complement(3737..>4513)
                     /locus_tag="VGA15_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9587577.1"
                     /translation="RDDEILGLVRSRYGATSVDQQDVIRAEVEAATAEGDVVRRQGEL
                     KSAAARLNALIGRPSLAPLAAPTGFRPVRAISLAQAQALARSANPMLAMAGAQSNSAS
                     QTKSLTDLNYYPDVTLGAKYVQRPGTEDTGEFMLGVKLPLHYEVKDAEQRAAGARLGA
                     AQARNEALRLRIDGQVADAWFSVDALRKVVQIYASRQVPAARASLDTARNGFQAGSTD
                     LFFVFEAERRLRTVQLDLLKLKVELQAKYAEMERLAGGSL"
BASE COUNT          868 a         1431 c         1340 g          874 t
ORIGIN      
        1 gtacgacatc cagatagtag ccgccgatga cacgttcagc aaaggcgctc gaggtgcccg
       61 gaactgcctt cacaaccgtt tcgatctgcc gtgccagctt ctccatctcc gtcaggtctt
      121 ttccaagaac cttgatgccg atcggcgtgc gaattccggt cgcgagcatg tcggtgcgcg
      181 ccttgatcgg catggtccag gcgttggaga cgccgggaaa ctgcaaagcc ttgtccagct
      241 cggcgattag gccatcgacg gtcaggccgg cacgccattg cgcctttggt ttgagattga
      301 cgatcgtctc aaacatctcg gtgggagcgg gatcggtcgc ggtctcagcc cgtccggcct
      361 tgccataaac cgactcgact tcgggaaacg tctttatgac cttgttctgc gtttgcaaaa
      421 tctccgccgc cttggttacc gagagccccg gcaacgtcgt cggcatatac atcagcgtgc
      481 cctcgttcag gctcggcatg aactcggtgc cgagctgccg tgccggccag aggctgaccg
      541 ccaaggcgac aagcgcgaga aagatcgtga gggtcttcgc tttcaacacc ccctgaatca
      601 gcggccgata gacccagatc aggaaccggt tgatcgggtt cttgtgctcc ggaatgatct
      661 tgccgcgaat gaacaacacc atgagggccg gaaccagagt gatcgacagg accgccgccg
      721 cagccatcga gaacgtcttg gtaaaggcga gcgggctgaa caggcgcccc tcctgcgatt
      781 ccatggtgaa gatcggcagg aacgacaccg tgatgatcag caggctgaaa aacagcgcgg
      841 gaccgacctg gctcgccgcc tcgatcagga cttcaaggcg cggcttgtcg ggcggcgcgc
      901 gttcgagatg cttgtgagcg ttctcgatca tgacgatggc gccgtcgatc atggcaccga
      961 tcgcaatcgc gatgccgccg aggctcatga tgttcgaacc gatgccgagc gccttcatgg
     1021 cggcgaacgc catcaggatg ccgaccggca acatcacgat ggcaacgaga gcgctgcgca
     1081 catgcagcag gaacgcgatg cagacgagcg cgacgacgat gctctcctcc gtgagcgtcc
     1141 gcttcagtgt ctcgatcgca ttgtgaatca gattggagcg gtcatagacc ggtatgatct
     1201 cgacgccttt cggcagggcc gacgccatat cggcaagccg cgccttcaca ttgtcgatga
     1261 catcaagcgc gttctggccg aaacgttgca gcgcgatgcc gctggcgacc tctccctccc
     1321 cgttcagctc ggtgataccc cggcgctcgt ccggaccgaa ctcgatccgc gccacatctt
     1381 tcagcaggac cggggtgcct ttatccacct tcacaacgat gttcgataga tcggaaaggc
     1441 ttttgacata accgcggccg cgcacgatga attcgaattc cgacaactcc acactacgtc
     1501 cgccgacatc catgttgctg ccgcggaccg catcgcgcac tttcgcaagc gggataccca
     1561 gcgaccgcat ccggttcgga tcgaggatga tgttgtattg cctgacgaag ccgccgacgc
     1621 tggcgacttc ggcgacgccc tctgccttgg caatgccgta gcggatggtc caatcctgca
     1681 gggatcgcag gtcggccagc gatttatcct tggactgcag cgcatattga tagacccacc
     1741 cgaccccggt cgcatccggc cccagcgtag gcgtcacacc attcggcagc ctgcgcgccg
     1801 ctgcgttgag atactcaagg acgcgcgagc gcgcccaata gatatcggtg ccatcctcga
     1861 agatgatgta gacgaacgag acgccgaaga aggaaaagcc gcgcaccact ttcgattttg
     1921 gcacggtgag catggccgtc gtcagcggat aggtcacctg atcctcgatg acctgtggcg
     1981 cctggccctg atattcggta tagacgatga cctgggtgtc ggagagatcg ggaatggcgt
     2041 ccagcgccga gtgctgcagg gcatagagcc cggccgccgc agcgaagacc gcaccgatca
     2101 ggaccagcat caggttgcga gcggaccagt caatgagccg cgcgatcatt gcgatttctc
     2161 cccggaatcc aggccactga gagcggcttt gagattgctt tcggcatcga tcaggaagtt
     2221 cgcggagacg acgaccttgt cgttttcagc gagcccttcc ttgatctcga tgcgaccgtc
     2281 gccgcggcgc ccaagcttga cggcgcgcgg ttcgaaacgg ccgtctccct tgtcgagcag
     2341 cacaatttgc cgctcgccgc tgtcgatgac cgcgctgctc gacacggtga caacaggagc
     2401 ttccgttcca gtggcgattt cgacatcggc atacatgtcg gggcgcagca cctcgtccgg
     2461 atttggcaat tcgattctga cccgcgctgt ccgcgtctcc gccatcaagt gcggatagat
     2521 caacgcgact tttccgttga aaacccgatc gggataggcg cgaagccgga cgccggcctt
     2581 ttgtccgacc tcgaccaggg aaagatcccg ctccgcgaca tcgaccaata cccagacgac
     2641 ctggtgatcc gcgatccgga acagaacatc gccgggaccg gcgcgcatgc cgttgacggc
     2701 tgcgcgctcc acgatctcgc cgtcctgcgg tgcaggccag ctcgcataga ccgggatttc
     2761 gcgcgtgcgc tcgatgtcgg caataaactt ctcaggggca tccagatttt caaggcgacg
     2821 acgcgcgcct ttcaaggctt ggttgctgat gccggcgtcc ggacgggcat tgagcgccga
     2881 cagatattcg gcggcggcgc tcgacaggtt cgggccgtaa acgcgcatca gcggctggcc
     2941 cttatggaca tgcgtgccgg tggtgacgtt ttcgacgctg tcgatgaagc cttcgaaacg
     3001 cagcgatacc accgatctgc ggcgctcgtc ttcctgaaca gtgcccggtg cacgaacaac
     3061 cgtgttcagg gtgcgccgtt ccgcgacctc ggtctgaacg cctgcctttt gcaacttgcc
     3121 gacgctgacc ttgaccgacg aatcgtcgtc ctgttcgccc tcatagacgg gcagatagtc
     3181 catccccatc gaatctttct tcggcgtcgg cgacgtatcg ggaaggccca tcgggttgcg
     3241 gtaaaagcgg attttgcctc gatccccacc cttcgcggtc atagtttccg gagccttctc
     3301 ctcaaagctc acgtcttcgc tggcacggac agctgaataa tccttgcctg aggatgtttt
     3361 cttgggcgtc agcgaatagt ccggtagacc gtccggatca cgatagtaga tgatggggcc
     3421 gctcgcttcg gactgagcca tgctctgttt tgcagccacg ggcagaacac catcgagcca
     3481 tccgggaagc ggaccgttcg agcgtccgac ggctaatccg accactgcgg cagccagaat
     3541 cgcggacaca atcaggaaag cgcctactgg ccgtctcata gcgaacctcc cgccaagcgt
     3601 ttcatctcag agcgtttgaa tctggcgggc ccttgcgcag gagaagtgca tcatgtgcgc
     3661 ttcctcagtg aattcggagc aaatcgccgg ccggttacat gttcacgccg acgtgaagcc
     3721 gcccaatacg ttcctctcat agcgaacctc ccgccagacg ctccatctcg gcatatttcg
     3781 cctgcagctc caccttgagc ttcagcaagt cgagctgcac cgttcgaagc ctgcgctccg
     3841 cttcgaatac aaaaaacaga tcggtggagc cggcctggaa gccatttcgt gcggtatcga
     3901 gtgaagcgcg cgccgcgggg acttggcgcg acgcgtagat ctgcacgacc ttccgcagcg
     3961 catcgacgct gaaccaggca tcggcgacct gtccgtcgat ccgcaggcgc aacgcctcgt
     4021 tgcgggcttg cgccgcgcca agacgcgccc ctgccgcacg ttgctcggca tccttgacct
     4081 catagtgcag cggcagcttg acgcccagca tgaactcgcc ggtatcttcg gtgccgggcc
     4141 gctgcacgta tttcgcgccg agcgtcacgt cggggtagta gttgagatcg gtaagagact
     4201 tggtttggga ggcggaattg ctctgcgccc cggccatcgc cagcatcggg ttcgcggagc
     4261 gggcgagcgc ctgagcctgc gcgaggctga tcgccctgac gggccgaaag ccggtcggcg
     4321 cagccaacgg agccaacgac ggacggccga tcagtgcatt cagcctggcc gcagcggatt
     4381 tcagttcccc ctgccggcga acgacgtcgc cctcggccgt ggcagcttcc acctcggctc
     4441 tgatgacgtc ctgctgatcg accgacgttg cgccatatcg tgaccgcacc aatccgagga
     4501 tttcgtcatc gcg
//
LOCUS       DASWGM010000249         3281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_23242,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000249 DASWGM010000000
VERSION     DASWGM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23242"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /gene="hydA"
                     /locus_tag="VGA15_07520"
     CDS             complement(<1..1151)
                     /gene="hydA"
                     /locus_tag="VGA15_07520"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX9587578.1"
                     /translation="MAPLIIRGGTVVNHDHSRRADIVVDGEAIVAVGAAVDAPAGAEI
                     IDAGGCYVMPGGIDPHTHLEMPFMGTVTADDFESGTKAALAGGTTMVVDFCLPDPGQS
                     MLAAYQDWRRKSERAASDYGFHMAVTSWSKQVFDEMEIVVKTYGINTFKHFMAYKGAL
                     MVNDDELYNSFARCAHLGAMPLVHAENGDVVARMQEALLERGVTGPEGHAYSRPPEVE
                     AEATNRAIMIADMTGAPLYVVHTSCREAHEAIARARANGKRVYGEPLIQHLLLDATEY
                     ENKSWNHAARRVMSPPFRARAHQDSLWAGLQSGSLQVVATDHCAFTTEQKRLGAEDFR
                     KIPNGTGGLEDRLPLLWTAGVTTGRLTKEEFVAVTSSNIARILNIYPRKG"
     gene            1439..>3281
                     /locus_tag="VGA15_07525"
     CDS             1439..>3281
                     /locus_tag="VGA15_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX9587579.1"
                     /translation="MDVPSSAPVGGLIDRSVTGVGPSRSEAVLEQAKYLHHAGSPWFL
                     ALTALGVVFGDIGTSPLYAFQVALTGVGHSPPTPADVLGIVSLILWALTVMVSLKYVV
                     FVLRADNDGEGGILALLSLVAADQVANGARLPVLVLLGVIGASLLYGDGVITPAISVL
                     SAMEGLKLVAPGFEHFVVPATIAVLIGLFVIQRYGTGSIGKLFGPIMVIWFVVIGGLG
                     AANIWTAPAILRAISPTEAVGFLIADPKISFVVIGAVFLALTGGEALYADMGHVGANA
                     IRRAWFGLVLPALLLNYFGQGALILSDPTAADNPFYKLAPGWALIPMVVLATFATIIA
                     SQALVSGVFSLTRQAMQMGLCPRARITPTSVDEAGQIYVPAANWLLMTGTLLTVVLFR
                     SSENLAAAYGIAVSGTMLITTILLYRVAVSRWQWPPTVAIPIIAIFGAIDATFLVSNS
                     IKIVEGGWFPLIVGGLIAVLMLSWRKGSSEVRHRLHEMSVPLKEFIDYANNTCIGRAP
                     GMGVWLTKVEHGASPMLLRHIEHNRVLHATVVLLTFVSDRRPRVPFHERHSVHRLGHG
                     FYRIQVRLGFMQTPDIPLTLINCNRLGFDADLDHKNYYIAHETIVRRA"
BASE COUNT          549 a         1024 c         1043 g          665 t
ORIGIN      
        1 ccttttctcg gatagatatt gaggatgcgg gcgatgttcg aagacgtcac cgcgacgaat
       61 tcttctttgg tcagccggcc agtggtgacg ccggcggtcc agagcagtgg caggcggtct
      121 tcgaggccgc cggtgccgtt cgggatcttg cggaaatcct cggcgccgag acgcttctgc
      181 tcggtggtga acgcgcagtg gtcggtcgcg accacctgca gcgagccgga ttgcaggccg
      241 gcccaaagac tgtcctgatg cgctctggcg cggaacggcg gtgacatcac gcgccttgcg
      301 gcatgattcc agctcttgtt ttcatattcg gtggcatcga gcagcagatg ctggatcagc
      361 ggctcgccgt agacgcgctt gccattggcg cgggcccgcg caatcgcctc atgcgcctcg
      421 cggcagctcg tgtgcacgac atagagcggc gcgcccgtca tgtcggcgat catgatggca
      481 cggttggtcg cctcggcctc gacctccggc gggcgcgagt aggcgtggcc ctcggggccg
      541 gtgacgccgc gctccagcag ggcttcctgc atgcgcgcga ccacgtcacc gttctcggcg
      601 tgaaccagcg gcatggcgcc gagatgggcg cagcgcgcga acgagttgta aagctcgtcg
      661 tcattgacca tcaacgcgcc cttataggcc atgaagtgct tgaacgtgtt gatgccgtag
      721 gtcttgacga cgatctccat ctcgtcgaac acctgtttcg accacgaggt cacggccatg
      781 tggaagccgt aatcgctcgc tgccctctcg gatttgcgcc gccagtcctg atacgccgcc
      841 agcatcgatt gcccgggatc cggaaggcag aaatccacga ccatcgtggt gccgccggcc
      901 agcgccgcct tggtgcccga ttcgaaatcg tcggcggtga cggtacccat gaagggcatt
      961 tcgagatggg tatgcggatc gatgccgccc ggcattacgt aacaaccgcc ggcgtcgata
     1021 atttcggcgc cagcgggggc gtcgacggca gcgccgaccg cgacgatcgc ctcgccgtcg
     1081 acaacaatgt cggcgcgccg cgaatgatca tggtttacga ccgtaccgcc gcggatgatg
     1141 agaggggcca taggaactcc gaacagacaa ctccagacag gcaattcgaa aagcggcatt
     1201 gcgcgtccgc aatgctaccg ttcccggcag cctaattcgc gcttaaaagg tgagcaacca
     1261 cgtcacctgt catatccgaa gctcaggccg acgacgaaag tgtcgcgcga cacaagcgga
     1321 gtcggctgcc gccgcgtctg gccgaccgct attggatggc gcgaccgagt ttgatgacat
     1381 ggcctaaatc ttgaatgcca aaacccgcga gcacacacaa tgtggtcggg aggctgacat
     1441 ggatgtacca tcgagtgccc cggtcggcgg cttgatcgac cgttcggtga cgggtgtcgg
     1501 tccgagccga tccgaggctg tgctcgaaca agccaaatac ctccatcatg ccggcagccc
     1561 ctggtttttg gcactgaccg cgctcggcgt cgtgttcggc gacatcggga ccagcccgct
     1621 ctacgcattc caggtggcgc tgaccggcgt cggtcattcg cctccgacac ccgccgacgt
     1681 tctcgggatt gtctccctga ttctctgggc gctgacggtg atggtgtcgc tgaaatatgt
     1741 cgtgttcgtg ctgcgcgccg acaatgacgg cgagggcggt atcctggcat tgctctcgct
     1801 tgtcgccgcc gatcaggtcg ccaatggcgc aagacttccc gtgctggttc tgctcggcgt
     1861 gatcggcgcc tccttgctct atggcgacgg cgtcatcacc ccggcgatct cggtgctttc
     1921 ggcgatggaa gggctgaagc tggtcgcgcc ggggtttgaa catttcgtgg tgccggccac
     1981 gatcgcggtg ctgatcggat tatttgtgat ccagcgctac ggcaccggca gcatcggaaa
     2041 actgttcggc cccatcatgg tgatctggtt tgtcgtcatc ggcgggctgg gcgccgccaa
     2101 tatctggacg gcgcccgcca ttcttagggc gatcagccca accgaggctg tgggcttttt
     2161 gatcgctgat ccaaaaatct cgtttgtcgt gatcggcgcc gtgttcctgg cgctgaccgg
     2221 cggcgaggcg ctgtacgccg acatgggcca tgtcggcgcc aacgccatcc gccgcgcctg
     2281 gttcgggctg gtgctgccgg cgctgctgct gaattatttc ggccagggcg cgctgatcct
     2341 cagcgatcca acggctgccg acaatccgtt ctacaaactg gcgccgggct gggcgctgat
     2401 cccgatggtg gtgctggcca ccttcgccac catcattgcc tcacaggcgc tggtctccgg
     2461 cgtgttttcg ttgacgcggc aggccatgca gatgggatta tgcccgcgcg ccaggataac
     2521 gccgacctcg gtcgatgagg caggccagat ttacgtgccc gccgccaatt ggctgttgat
     2581 gaccggcacg ttgctcaccg tggtgctgtt ccggtcgtcg gaaaatctgg cggccgccta
     2641 tggcattgcc gtgtccggca ccatgctgat caccacgatc ctgctctacc gcgtggcggt
     2701 gtcgcgatgg cagtggccgc ccaccgtcgc gatcccgatt atcgcgatct tcggcgcgat
     2761 cgatgccacg ttcctggtat cgaattcgat caagatcgtc gaaggcggct ggtttccgct
     2821 catcgtcggc gggttgatcg cggtcctgat gctgtcgtgg cgcaagggct cgtcggaagt
     2881 caggcatcgc ctccacgaga tgtcggtgcc gctgaaggaa tttatcgatt atgccaacaa
     2941 cacctgcatc ggccgcgcgc cgggaatggg ggtatggctc accaaggtcg agcacggggc
     3001 ctcgccgatg ctgctgcggc acatcgagca caaccgggtc ttgcatgcga ccgtggtttt
     3061 gctgacgttc gtgtccgacc gccggccgcg ggtgccgttc cacgagcgcc attcggtgca
     3121 tcggctcggt cacggttttt atcgaatcca ggtcaggctg ggattcatgc agacgccgga
     3181 tattccgctg accctgatca actgcaacag gctcggcttc gacgccgatc tcgaccacaa
     3241 gaactactac atcgcccacg agaccatcgt tcgccgcgcc g
//
LOCUS       DASWGM010000250         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_986702,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000250 DASWGM010000000
VERSION     DASWGM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986702"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VGA15_07530"
     CDS             complement(<1..673)
                     /locus_tag="VGA15_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9587580.1"
                     /translation="MNERRYRLIGSTASPYAVKLRAILRYRRIPFDWVIMTKALRKQA
                     GHLRPNLIPVLQYPDGSFRGETTALAYDLEARHPDRSVIPEDKAIAFVCDLLEDLADE
                     WAVKPLFLYRWWDPDDQAYVSRWAAEEWSVSDAEPGSAEEIEHFRIRQISRMPVLGAT
                     AENKPLLEESYRQILAAFEPHVGMTKYLFGSRPSLADFAWFGQLSELATDPSPMRIMR
                     EKAPFT"
     gene            945..1757
                     /locus_tag="VGA15_07535"
     CDS             945..1757
                     /locus_tag="VGA15_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9587581.1"
                     /translation="MKGPEDDSGLTDRVAIVSGGGAAGDGIGNGRAASILLARAGTKV
                     LVADLDLRLAERTVEMIQAEGGTAAAQSGDITNESDCERLVDAAVDRWGRLDFLDNNV
                     GIGSRGSVVDEKPEEYRRVMQINVETMFLLSKYAIPAMIKTAGGGSIVNISSISALRP
                     RGLTTYTTSKAAVIGLTQAMAVDHGRDNIRVNCICPGPMYTPMVYARGMSDAARAQRA
                     KASLLKTEGTGWDVGHAVKFLLSNYARYITGQVLVVDGGVTLQGPERDSRDH"
     gene            1785..>2221
                     /locus_tag="VGA15_07540"
     CDS             1785..>2221
                     /locus_tag="VGA15_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9587582.1"
                     /translation="MARLPYLEADQVAPEYRDMLKRNTNLHKLLVNSPDMARAFNGIG
                     GYIRFKSKLDPRLRELAILQVGWLEKSEYEFTHHVKIGKEFGVTDEDIRGLIAETEGK
                     PSQLEPLAKAIVKGAREMVRELAMSDATFAEIKQELSDEHMTDL"
BASE COUNT          473 a          689 c          663 g          396 t
ORIGIN      
        1 cggtaaacgg cgctttctcc cgcatgatcc gcatcggact gggatcggtc gccagttcgc
       61 tcaattggcc gaaccatgca aaatccgcca gcgacggccg gcttccgaac agatattttg
      121 tcattccaac gtgcggctcg aacgccgcca ggatttgacg atagctctct tccagcaaag
      181 gcttgttttc ggccgtcgcg ccgagaacgg gcatgcggga aatctgcctt atgcggaaat
      241 gctcgatttc ctccgcactc ccgggctcgg cgtccgacac cgaccattct tcagctgccc
      301 atcgcgagac ataggcttga tcgtcggggt cccaccaccg atagagaaaa agcggcttca
      361 cggcccattc gtcggcgagg tcttcgagga gatcgcagac gaacgcgatg gccttatctt
      421 cgggaatgac cgaccgatcc gggtgccggg cttcgagatc gtacgccagt gccgtggttt
      481 cgccgcgaaa gcttccgtcg ggatattgca gaacaggaat caaattcgga cgcaggtgcc
      541 cggcctgttt ccgcagcgcc ttggtcatga tgacccagtc gaacgggatc ctccggtagc
      601 gcaatatcgc ccgcagcttg accgcgtagg gcgatgccgt ggatccgatc agccgatagc
      661 gtcgttcatt catgcaagat gtcctgacta ccgggcgcga tcacaccgat cgcgcttcac
      721 attcgccatc gccgcgtgtt tctgcctaac ccaggctctg aacccgtgca accggtcgtc
      781 accttcgctg aaagggttgg aaagctgacg agctctgcat tttacgaagg cggaccgtct
      841 cccacgtaag caacgcggac cttgttgtat cgcgtgaaat catccctaat gcgcggaagg
      901 tcatgcaaca agaaatgcag aaagcgacca aagggagaag cgcgatgaag ggtcccgagg
      961 acgattccgg cctgaccgac agagtggcca tcgtgagcgg cgggggtgct gcgggcgacg
     1021 gcattggtaa cggccgggcg gcgtcaatcc tgctggcccg cgcgggcacc aaggtgctgg
     1081 tggccgatct cgacctcagg cttgccgaac gcacggttga aatgatccag gctgagggcg
     1141 gcacggcggc ggcacagtcg ggcgacatca ccaacgaatc cgactgcgag cggctggtcg
     1201 atgcagctgt cgaccgctgg ggccggctcg acttcctcga caacaatgtc ggcatcggca
     1261 gccgcggcag cgtcgtcgac gagaagcccg aggaatatcg acgggtgatg caaatcaacg
     1321 tcgagaccat gttcctgctc tcgaagtacg cgattccagc aatgatcaaa accgccgggg
     1381 gcggctccat cgtcaacatc tcgtcgatat ccgcgctccg gccgcgcggc ctcaccacct
     1441 acacgacctc gaaagccgca gtcatcgggc tgacccaggc gatggcggtc gatcacggcc
     1501 gcgacaacat ccgggtcaat tgcatctgcc ccggtccgat gtacacgccg atggtctatg
     1561 cgcgcggcat gagcgacgcc gcacgcgccc aacgcgccaa agcctcgctg ctgaaaaccg
     1621 agggaaccgg ttgggacgtc ggccacgccg tgaaattcct gctcagcaac tacgcccgct
     1681 acatcaccgg gcaggtgctg gtagttgacg gcggcgtcac gctgcaaggc ccggaacgcg
     1741 attcacggga tcactgatca cttcataggg ggagaacgaa cacgatggcc cgcctgccct
     1801 atcttgaggc cgaccaggtc gcgcccgaat atcgcgacat gctcaagcgc aacaccaatc
     1861 tgcacaagct cttggtcaac tcgcccgaca tggcgcgtgc cttcaacggc atcggcggct
     1921 acatccgctt caagagcaaa ctcgacccgc gcctgcgcga acttgctatc cttcaagtgg
     1981 gctggctgga gaaatcggaa tacgaattca cccatcatgt gaagatcggc aaggagtttg
     2041 gcgtcaccga tgaggatata aggggcctga tagccgagac cgagggcaag ccctcccagc
     2101 ttgagccgct tgccaaggcg atcgtgaaag gcgcacgcga aatggtgcgc gagcttgcga
     2161 tgtcggatgc aactttcgcc gagatcaagc aggaactctc tgacgagcac atgaccgatc
     2221 t
//
LOCUS       DASWGM010000251         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1960804,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000251 DASWGM010000000
VERSION     DASWGM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1960804"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1437)
                     /locus_tag="VGA15_07545"
     CDS             complement(280..1437)
                     /locus_tag="VGA15_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HEX9587583.1"
                     /translation="MPETLITPFSRWILVCAALFGVAALSVDASAQMSQAPPAPPQQG
                     APVNPMCPRLEAQLATIDRGGGDPAKDEQIRRYQDAAAKQQSELDRVTSQAKRMGCDS
                     SGFFSLFSGQSAQCGPVNNQIQQMRANLEQITTSLERLRTGGLGGSDRDNQRRSVLTA
                     LAQNNCGPQYANAARGPGNFLETLFGNNNNNPVAPPGADLGAQSGTYRTVCVRSCDGA
                     YFPISFATVPGRFPDDERTCKALCPAAEATLYSYRNPGEDMNQAVSINGQPYSSSPNA
                     FRYRQEFNPSCACKAAGQTWSDALKSIDDKAEAEQQGDIIVTEESAKKMARPLTKGVA
                     TSKKGAATAATTATAPATPAPEASPPTADSAGAGPDNKPIRSVGPTFIPAR"
     gene            complement(1714..2022)
                     /locus_tag="VGA15_07550"
     CDS             complement(1714..2022)
                     /locus_tag="VGA15_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587584.1"
                     /translation="MRSLPALSACMFLLNASAASADSRVFIIANQADGYGVDQCLARG
                     EQCGAHAARSYCQSRDFTQASAYRRVDPDEITGSVPKPAGGSCSHAGCDEYVAITCQR
                     "
BASE COUNT          432 a          696 c          744 g          458 t
ORIGIN      
        1 ccgggcgtca aggtcaccca gaagaatttt gggcgcgacc gccgttatcc catcaccaac
       61 aagtttcgcg attcagccaa ggcgctgccg gagcccgacg atacgctagt ctcacgcgcc
      121 agccgcggct cggcggaagc gttcgagggg tagattagag catgatcccg aaaagcatgc
      181 cctcggactt gatctggggg tggataccgg ttttcggaaa agatcatgct caaccaaaga
      241 taaggcgccg gttctgattc agcgaagctg gatcagaacc tagcgcgcgg ggataaaggt
      301 cgggcctacc gaacgaatcg gcttgttgtc cggcccggcg ccagccgaat cggccgtggg
      361 gggcgatgcc tcaggtgcgg gcgttgccgg cgcggtcgcc gtggtggctg cggtcgcggc
      421 gcccttcttc gacgttgcca cccctttggt cagcggccgc gccatcttct ttgcgctctc
      481 ttcggtaacg atgatgtcgc cctgttgttc ggcctcggcc ttgtcgtcga tggacttcaa
      541 tgcgtcggac caggtctgtc cggcggcctt gcaggcgcag gatggattga actcctgacg
      601 gtagcgaaac gcgttgggcg acgacgaata gggctggccg ttgatcgaca ccgcctggtt
      661 catgtcctcg ccgggattgc gatagctgta gagggttgcc tccgccgccg ggcaaagcgc
      721 cttgcaggtc ctttcgtcat cggggaaacg gccgggcacg gtggcgaacg agatcggaaa
      781 gtaagcgccg tcgcagctgc ggacgcagac cgtgcgatag gttccggatt gcgcacccag
      841 atcggcgccg ggcggcgcca ccggattgtt attgttgttg ccgaacagag tttccaggaa
      901 attgcccggg ccccgcgcgg cattggcata ttgcggtccg caattgttct gcgcgagcgc
      961 ggtcaggacc gaacggcgct gattgtcgcg atcggagccg ccgaggccgc cggtacgcag
     1021 ccgctcgagg ctggtcgtga tctgttcgag attggcgcgc atctgctgaa tctggttgtt
     1081 gaccggaccg cattgcgccg attgaccgct gaacagcgag aaaaatcccg aactgtcgca
     1141 gcccatgcgc ttggcctgcg aggtaacgcg gtcgagttcg ctttgctgct tggccgcagc
     1201 atcctggtag cggcggattt gctcgtcctt ggccggatcc ccgccaccgc ggtcgatggt
     1261 cgccaattgc gcttccagcc gcgggcacat cggattgacg ggcgctccct gctgcggcgg
     1321 cgccggcggc gcctgactca tttgcgccga agcgtcaacg ctcagcgcgg ccacgccgaa
     1381 cagcgcagca cagaccaaaa tccatctgga aaagggggta atcagcgttt caggcatatc
     1441 cggccatctc atcataatcc gattagatta gatcggatca tgatgcgatc tctttgtttg
     1501 cgcataatgt tttcgaaaaa ccggtctcca cccccggatc aagtccgagg gcatgctttt
     1561 cgggatcatg ctcgagaaca cctccgcgcg ggcttaagat accgaaaggc gcggccaacg
     1621 cggccctcac atatcggctt ctcggggcac cgtcacgtcg tttccgcggc gccggtgtgg
     1681 cgggcccgac cttcgatcac cgcgacggcg gctttagcgt tgacaggtaa tggcgacgta
     1741 ttcgtcgcag ccggcatggc tgcagctgcc accggcgggt ttgggaactg accccgttat
     1801 ttcatccgga tccacgcgcc gataggccga ggcctgcgtg aaatcccgcg actggcaata
     1861 ggaccgggcc gcgtgggcgc cacattgttc gcccctcgcg aggcactgat cgacgccgta
     1921 accgtccgcc tggttggcga tgatgaaaac gcggctgtca gccgatgccg cggaagcgtt
     1981 gaggaggaac atacaggccg aaagcgcagg gagggatcgc atgctgcacc ataaaaaaat
     2041 gaggggaccg cccctcattg gataaaccca aaacgttaat aatgattaac catggcggcc
     2101 ggggcggatt gatggcaaga atccgtcatt agcaagctct tgacgccgtc ccccgggctg
     2161 gcccatggta ggggcatgca cggtctcctc gccatttgtg gcatttgccg cgagattatg
     2221 agctagcgat tcgctggctg aggccgtctt ttctcattcg agatcactgg acgcccggcc
     2281 gcaagggacg ggatccatgg acttacgcct ctacgatacc ttgaccaagg
//
LOCUS       DASWGM010000252         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1686855,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000252 DASWGM010000000
VERSION     DASWGM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686855"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGA15_07555"
     CDS             complement(<1..533)
                     /locus_tag="VGA15_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9587585.1"
                     /translation="MTTDHQIVLSVTGLTKSYRSAGEQVVVLRGVNLRIAAGERVALT
                     GESGSGKSTLLHLIAGLDKADGGEIRLDDISVSDLPDAGRAALRRDRLGLVFQQFNLI
                     PSLIVEDNLKFQSRIASRHDAAWHAELVERLGLGGLLKRYPEQLSGGQQQRVAIGRAL
                     APRPLLLLADEPTGNLD"
     gene            complement(553..1326)
                     /locus_tag="VGA15_07560"
     CDS             complement(553..1326)
                     /locus_tag="VGA15_07560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9587586.1"
                     /translation="MTRPLYRRPRAIGMLASLSILAGIGAVSAMAQDLKTPDYDAIVA
                     SPDRGDGDRQADQRRQPARMLAFAGVKTGMKVLDMEANAGYSTELLARAVGPTGVVYA
                     QDSSAVIERFVKDKFDIRAQKQAMNNVVHLVRNFDDPIPPEVSGLDMITFFFAYHDVT
                     YMEVDRAEMNRKMFAALKPGGFLVIADHSARPGDGTAVAKTLHRIEESVLRQEIEAAG
                     FKLATEADFLRHPEDPRDAAVFRPQIPTDEFVLKYLKPL"
     gene            1601..>2718
                     /locus_tag="VGA15_07565"
     CDS             1601..>2718
                     /locus_tag="VGA15_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587587.1"
                     /translation="MTTQPTPKGAWKITFLLFLFMLVNFADKIVVGLAGVPIMTELKL
                     EPEQFGLLGSSFFFLFSIAAIVVGFIVNRIATRWVLLALALVWALAQFPMVGTVSFST
                     LLICRVILGAGEGPAFSVATHSVYKWFPDEKRTLPTAILSQGSAFGVILAVPALNWVI
                     VNYSWHYAFGALGVVGLMWVVAWLILGKEGPLVQTYAMAAADPRVPYFQLLTSRTFIG
                     CCTATFGAYWALSLGLTWFTPFIIKGLGFSQKDAGWISVLPWVFGAVIVLLTGWISQV
                     LMARGVTTRGARGVLGSVPLIVGGLILVVMPYADGAGLQIALLVAGTGLSGSIYMVCP
                     PMLGEFTPVAQRGAVISIYSAIYTLAGILAPSVMGSVI"
BASE COUNT          456 a          831 c          864 g          567 t
ORIGIN      
        1 tcgtcgaggt tgccggtggg ctcgtcggcg agaagcagca gcggtcttgg cgccagcgcg
       61 cggccgatcg cgacccgctg ctgctggccg ccggataatt gctcgggata acgcttcagt
      121 aaaccgccga gcccgagccg ctccaccagt tcggcatgcc aggcggcgtc gtgacggctg
      181 gcaatgcgcg actggaactt gagattatct tcgacaatca ggctggggat caggttgaac
      241 tgttgaaaga ccagcccgag ccgatcgcgc cgcaaggccg cccggccggc atccggcaga
      301 tccgacaccg atatgtcgtc cagcctgatc tcgccaccgt cagccttgtc cagtccggcg
      361 atcagatgca gcagcgtgct cttgccgctg ccggattcgc ctgtcagtgc gacgcgctcg
      421 cccgccgcga tgcggaggtt caccccgcgc aacaccacga cctgctctcc cgccgagcga
      481 tagctcttgg tcagaccagt gacgctcagc acgatctgat ggtcggttgt catgcgacgc
      541 gtgcgcgatg cttcacaacg gcttcaaata tttcagaacg aattcgtcgg tcggtatctg
      601 cggacggaac accgcggcat cccgcggatc ttccggatgg cgtaggaaat cggcctcagt
      661 tgcgagcttg aagccagcgg cctcgatttc ctggcgcagc acgctttcct cgatccgatg
      721 cagggtcttt gcgaccgccg tgccgtcgcc gggacgcgcg gaatggtcgg cgatgacaag
      781 aaagccaccg ggcttcaggg cggcgaacat cttgcggttc atctcggccc gatcgacctc
      841 catgtaggtg acgtcgtgat aggcgaagaa aaacgtgatc atatcgagcc cgctgacttc
      901 gggcgggatc ggatcgtcga aattccgcac gagatgcacg acgttattca tggcctgttt
      961 ctgggcgcgg atgtcgaact tgtccttgac gaaccgctcg atcacggcag atgagtcttg
     1021 cgcgtacacc acgccggtcg gcccgaccgc gcgcgccaag agctcggtgc tgtagccggc
     1081 gttcgcctcc atatccagca ccttcatgcc ggtcttgacg ccggcgaagg cgagcatcct
     1141 ggcgggctgg cgacgctgat cggcttgccg atcgccgtcg ccgcggtcag gcgaggcgac
     1201 aatggcatca tagtccgggg tcttgaggtc ctgcgccatg gccgagaccg cgcctattcc
     1261 tgcgagtatc gaaagcgaag caagcatgcc gatggcgcgc ggccgacggt agagcggtct
     1321 ggtcatgaaa atcccctgct atggcgcaca acataccaag accacaacga gagatcacgg
     1381 tctcaatcgc gacggcgacg cgatcgtgat caggttgcat ggcgctatct tgacatcgcc
     1441 gggaagtatt ctacggatga atgttgagtt tgcagtgcac gcgagcgggc agctttcaga
     1501 tctccgtcaa aaatgtccga ttgcgttggc ggcgacccaa tgggtagcat tgggtcaggc
     1561 cgatcaagcg aggccggcaa cgatgtctgg gggaacgacc atgaccacgc aaccgacgcc
     1621 caaaggcgcc tggaaaatca cgttcctgct gtttctcttc atgctggtga atttcgccga
     1681 caagatcgtg gtcggcctcg ccggcgtacc gatcatgacc gagctgaaac tcgagccgga
     1741 gcagttcgga ctgctcggct cctcgttctt cttcctgttt tcgatcgcgg ccatcgtcgt
     1801 cggtttcatc gtcaaccgta tcgccacgcg ctgggtgctg ctcgcgctgg ccctcgtatg
     1861 ggcgctggcg cagttcccga tggtgggcac ggtcagtttc tcgacgcttc tgatctgccg
     1921 cgtgatcctc ggcgccggcg agggaccggc gttttcagtg gcgacgcatt cggtctacaa
     1981 atggtttccc gacgagaagc gcaccttgcc caccgccatc ctgtcgcagg gctcggcgtt
     2041 cggcgtgatc ctcgcggtgc cggcgctgaa ctgggtgatc gtcaattata gctggcacta
     2101 tgccttcggc gcgctcggcg tcgtcggcct gatgtgggtc gtggcgtggt tgattcttgg
     2161 caaggaaggg ccgctggttc aaacctacgc catggcggcg gctgatccgc gcgttcctta
     2221 tttccagctt ctgacctcgc gaacctttat cggctgctgt accgcaacgt tcggggccta
     2281 ttgggcgctg tcgcttggcc tgacatggtt cacgcccttc atcatcaaag gcttgggctt
     2341 ctcgcaaaag gatgccgggt ggatttcggt gctcccctgg gtgttcggcg ccgttatcgt
     2401 gctgctgacg gggtggatct cgcaggtgct gatggcgcgc ggcgttacca cgcgcggcgc
     2461 gcgtggcgtg ctgggctcgg ttccattgat cgtcggcgga ttgatcctcg tcgttatgcc
     2521 atatgccgat ggcgccgggt tgcagatcgc gttgctggtg gccgggaccg gactttccgg
     2581 atcgatctat atggtctgtc cgccgatgct cggcgaattc acgccggtgg cgcagcgcgg
     2641 cgccgtgatc tcgatctaca gcgcgatcta cacgcttgcc ggaatcctgg cgccatcggt
     2701 catgggcagc gtgatcca
//
LOCUS       DASWGM010000253         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2517546,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000253 DASWGM010000000
VERSION     DASWGM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2517546"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VGA15_07570"
     CDS             complement(<1..950)
                     /locus_tag="VGA15_07570"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX9587588.1"
                     /translation="MAKIKVTNPVVELDGDEMTRIIWQYIKDKLINPFLDINLMYFDL
                     GMEYRDKTNDQVTIDAANAIKKVGVGVKCATITPDEGRVKEFGLKEMWKSPNGTIRNI
                     LGGVVFREPIICKNVPRLVPGWTKPIIIGRHAFGDQYRATDFKFPSKGTLTMKFVGDD
                     GKVIEREVYKSPSAGVALAMYNLDDSIADFARASFNQGLSKGYSVYLSTKNTILKVYD
                     GRFKDIFQEIFDAEFKAKFDEKKITYEHRLIDDMVAAAMKWSGGYVWACKNYDGDVQS
                     DTIAQGYGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYRE"
     gene            complement(1075..1791)
                     /locus_tag="VGA15_07575"
     CDS             complement(1075..1791)
                     /locus_tag="VGA15_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587589.1"
                     /translation="MLSAFIPALTYYPAFALAGTFVKPSGFLPQGITSQIMVWAAINA
                     LIALALMPFAPKRASRAGIIMPSIGIALSTIIVSYAALWLADLAFKIDFRFWIVALKL
                     MSAKQFLIFLIYLVPFTAFFVIALDLLHRNFSTLAAGRAALYLTNILALTLGFIVLLG
                     LQYGTLFLSGKLFNPLPDPGFVPLSTIVAIQFVPLLAIAAVIATFTWRRTGSSLPGAL
                     ICGLFITWYVVAGTATQAAF"
     gene            complement(1764..2801)
                     /locus_tag="VGA15_07580"
     CDS             complement(1764..2801)
                     /locus_tag="VGA15_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9587590.1"
                     /translation="MRSRWSLAIAGLVLIVAGGLLAHLTQTSGGIRIQDIRFAGAKGN
                     IMSALLYIPPNATAQTPAPGILAVHGYINSRETQDGFAIEFARRGYVVLDLDQSGHGY
                     SDPPAFANGFGGPDGLIYLRSLDIVDKDNIGLEGHSMGGWTVLAAAMAMPNDYKSMVL
                     EGSSTGKPFAADGTPGWPRNVALVFAQYEEFSSLMWGVDLARDVTQSPKLWALFGTHD
                     AVEPAKLYGDIALGTARVLYTPAMTHPAEHISHQAIGYSLDWFDKTLRGGTPRPAEDQ
                     IWFRKEIGTLIALIGFVVLSIGVFDGLLETPLFSHLRLPEIADGTMPAPSRPPAATGP
                     RRSCCPPSFPR"
BASE COUNT          554 a          825 c          929 g          560 t
ORIGIN      
        1 tgctcgcgat agtgccgcgt cacggtgccg tgggcggcct cggcttccac cgtcttgccg
       61 tccggcgtca tcaagaccga ggtcatcagg ccgagcgagc catagccttg cgcgatggtg
      121 tcggactgca cgtcgccgtc atagttcttg caggcccaga catagccgcc ggaccacttc
      181 atggcggcgg cgaccatgtc gtcgatcaga cgatgttcgt aggtgatttt cttctcgtcg
      241 aatttggcct tgaactcggc gtcgaaaatt tcctggaaga tgtccttgaa ccgcccatcg
      301 tagaccttga ggatggtatt cttggtcgac aggtacaccg aatagccctt tgacaggccc
      361 tggttgaacg aggcgcgggc gaaatcggcg atcgagtcat cgagattgta catcgccagc
      421 gcgacgcccg cgctcgggga cttgtagacc tcccgttcga tcaccttgcc gtcgtcgccg
      481 acgaatttca tcgtcagcgt gcccttgctg ggaaacttga aatcggtggc gcggtactgg
      541 tcgccgaagg cgtggcggcc gatgatgatc ggcttggtcc agccgggcac cagccgcggc
      601 acgttcttgc agatgatcgg ctcgcggaac accacgccgc ccagaatgtt gcggatggtg
      661 ccgttcggcg acttccacat ttccttcagg ccgaattcct tcacccggcc ttcgtccggc
      721 gtgatggtgg cgcatttgac gccgacgccg accttcttga tggcgttggc ggcgtcgatc
      781 gtgacctggt cattggtctt gtcgcggtat tccatcccca ggtcgaaata catcaggttg
      841 atatcgagga acgggttgat cagcttgtcc ttgatatatt gccagatgat ccgggtcatc
      901 tcgtcgccat cgagttcgac gacggggttg gtcaccttga tttttgccat ggcaggtcgg
      961 gcctttttgg aaaacagcgg tttggggcgg gcgggatgat tgcatccgcg ctatagcact
     1021 gccgacgcgc cggcgaaagc caaggcgtta ggcacattca gcccatcttt tggtttagaa
     1081 agccgcctgc gtagcggtgc ccgccaccac ataccaggtg atgaagagac cgcagatcag
     1141 ggcgccgggc aggctcgatc cggtacgccg ccaggtgaag gtcgcgatga cggctgcgat
     1201 tgccaacagc ggcacgaact ggatcgcgac gatggtggag agcggcacga agccgggatc
     1261 gggcagcgga ttgaacagtt ttcccgatag aaacagcgtg ccgtattgca ggccgagcag
     1321 cacgatgaag ccgagcgtca gcgccaggat attggtgaga tagagcgcgg cgcgtcccgc
     1381 cgccaaggtg gagaaattcc ggtgcagcag atccagcgcg atcacgaaaa acgccgtgaa
     1441 tggaacaagg tagatcagga agatcagaaa ctgcttggcg ctcatcagct tgagcgcgac
     1501 gatccagaac ctgaaatcga tcttgaaggc aagatcggcc agccacaacg cggcatagct
     1561 gacgatgatg gttgagagcg cgattccgat cgacggcatg atgatgccgg cgcggctggc
     1621 ccgtttgggc gcgaacggca tcaatgctag cgcaatcagc gcgttgatgg cggcccagac
     1681 catgatctgg ctggtaatgc cctgtggcag gaagcctgag ggttttacga aggtgccggc
     1741 cagcgcgaac gcgggatagt aggtcagcgc gggaatgaag gcggacagca cgaacgccgt
     1801 ggtccagtgg cggccggagg ccgcgacggc gcgggcatcg tgccgtcggc gatctcgggc
     1861 aatctcaggt gcgaaaacag gggcgtttca agcagcccgt cgaacacgcc gatcgagagc
     1921 acgacaaagc cgatcagtgc gatcagcgtg ccgatctcct tgcggaacca gatttgatcc
     1981 tcggccggcc tcggcgtgcc gccccgtagc gttttgtcga accagtcgag gctgtagccg
     2041 atcgcttgat gcgaaatatg ctcggccgga tgcgtcatcg cgggcgtgta gagcaccctg
     2101 gcggtgccca gcgcgatgtc gccatagagc ttggcgggct cgaccgcgtc gtgcgtgccg
     2161 aacagggccc agagtttcgg actttgggtg acatcgcgcg cgaggtcgac accccacatc
     2221 agactggaaa attcctcgta ttgcgcaaac accagcgcca cattgcgcgg ccagcccggc
     2281 gtgccgtcgg cggcgaaggg ttttccggtg gaagagccct ccagcaccat cgatttgtag
     2341 tcgttgggca tcgccatcgc ggcggcgagc acggtccagc cgcccattga gtggccctca
     2401 aggccgatat tgtccttgtc gacgatgtcg agactgcgca gataaatcag cccgtctggg
     2461 ccgccaaagc cattggcaaa ggccggtgga tcgctgtagc cgtgcccgct ctgatcgagg
     2521 tcgagcacca catagccccg gcgggcgaat tcgatggcaa agccgtcctg ggtttcgcgc
     2581 gaattgatgt agccgtgaac cgccaaaatg ccgggcgcgg gggtctgtgc ggtggcattt
     2641 ggcgggatgt agagcagggc gctcatgatg ttgcccttgg cgcccgcaaa gcggatatcc
     2701 tggatccgga tgcccccgga ggtctgcgtc aggtgggcca aaagtccgcc ggccacaatc
     2761 agaactagtc cggcgatcgc caatgaccat cggctgcgca tcaagacctc ccgtatgacc
     2821 cgcgccgttg aagccggctt tcaggttcgt ttttgcaggt tcgttttt
//
LOCUS       DASWGM010000254         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2241343,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000254 DASWGM010000000
VERSION     DASWGM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241343"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VGA15_07585"
     CDS             complement(<1..834)
                     /locus_tag="VGA15_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX9587591.1"
                     /translation="MAREALTDARGLRRYADAFCAIAIALWLAPFAAHAARSHLPNPP
                     ARPHLPYPHLTWPLEISGSQYAPVGWADIPGWSDDDHLSAYTAFRASCRPIAAQHALP
                     TDSKALGISLRDPCRAAKTLDISEGAKARRFFEEHFLPLRISRLGEDAGFVTGYYEPV
                     IDGSRSQTDVYTVPVYRRPSNLFVRGFKQGDAGLPNKGQVFRKIGRRKLVPYYDRAEI
                     EDGAIAGRGLEICWLKNQTDLLFTQIQGSARVRIEDGSTIRINYDAHNGYPYTAVGRI
                     LI"
     gene            complement(812..1516)
                     /locus_tag="VGA15_07590"
     CDS             complement(812..1516)
                     /locus_tag="VGA15_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEX9587592.1"
                     /translation="MDIYTIIFLALAVFIFLRLRNVLGQRTGNERPPYDGAARNMVQG
                     AQDNNVVPIPGAVIDQAPLAPTPDVVPPSDRWKGLAEPGTPLAQGLDAIAAQDSSFDP
                     RHFLSGARSAYEMIVLAFANGDRRALKDLLSAEVYESFETVIKDREKAEQKTETRFVS
                     IDKAELVGAEARDRAAQLTVRFVSQMVSVTRDKAGTIVDGNPDKVSDITDVWTFARDV
                     TSRDPNWKLVGTGSAH"
     gene            2012..2500
                     /gene="secB"
                     /locus_tag="VGA15_07595"
     CDS             2012..2500
                     /gene="secB"
                     /locus_tag="VGA15_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX9587593.1"
                     /translation="MTNGNGAPPEAAPPPQLNVLAQYTKDLSFENPNAPASLAPQAQQ
                     PAINIQINVSANNIAENEFEVTLSVEGKAENGGKVMFSFDLAYAGVFRLLNIPKQDLH
                     PMVMIECPRLLFPFAREIIATSVRDGGFPPLMLDPVDFVGLYRQNMERQAAAQAAQAK
                     PS"
     gene            complement(2528..>3167)
                     /gene="dnaQ"
                     /locus_tag="VGA15_07600"
     CDS             complement(2528..>3167)
                     /gene="dnaQ"
                     /locus_tag="VGA15_07600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX9587594.1"
                     /translation="RLVEIGCVEIFNRMPTGQTFHRHLNPERDMPAEAFAVHGLSGEF
                     LADKPLFAQVVDDFLEFIADAPLVIHNASFDIGFINAELDRIKRPSIARDRLVDTLLL
                     ARRKHPGVSNRLDDLCSRYAIDNSRRTKHGALLDAELLAEVYIDLIGARQSQLILAAE
                     TRDTRAGWDGEMPRRQRLAPLAPRVTEDDRAAHRAFVATLGDKPIWSDFLSA"
BASE COUNT          637 a          978 c          975 g          577 t
ORIGIN      
        1 gatcaggatg cggccgaccg ccgtataggg ataaccatta tgcgcgtcgt aattgatgcg
       61 gatggtcgag ccgtcctcga tgcggacccg cgccgagccc tggatttgcg tgaacagcaa
      121 atcggtctgg ttcttgagcc agcagatttc gaggcctcgg ccggcaatcg ccccgtcttc
      181 gatctcggcc cgatcgtaat acggcacgag cttgcgacgg cctatcttgc gaaacacctg
      241 tcctttgttg ggcaagccgg catcgccttg tttgaagccg cggacgaaca agttcgaagg
      301 acggcggtag accggcacgg tgtagacgtc ggtctggctc ctggacccgt caatgacagg
      361 ttcatagtag cccgtgacaa agccggcatc ttcgcccagc cgcgagatcc gcaagggcag
      421 aaaatgttcc tcgaaaaatc tccgcgcctt ggcgccctcg gagatgtcga gggttttggc
      481 cgcgcggcag ggatcgcgca acgagatgcc caacgccttg gaatcggtgg gaagtgcatg
      541 ttgcgccgcg atcggccggc agctcgcgcg aaacgccgtg tatgccgaga gatggtcgtc
      601 gtcgctccag cccgggatat cggcccaccc gaccggagca tattgactgc cgctgatctc
      661 gagcggccag gtgaggtgcg gatacggaag atgcggtcgc gcgggaggat tgggcagatg
      721 agaacgcgcg gcatgcgcgg caaacggagc cagccacaat gcgatggcga ttgcacaaaa
      781 cgcgtcggca tatcgccgca agccacgcgc gtcagtgagc gcttcccgtg ccaaccagct
      841 tccagttcgg atcgcgtgaa gtaacgtcgc gggcaaaggt ccagacatcg gtaatgtcgc
      901 tgaccttgtc cggattgccg tcgacaatcg tgccggcctt gtcgcgggtg accgaaacca
      961 tctgcgacac aaaccgaacg gtcagctgcg ccgcccgatc gcgtgcttcc gcacccacaa
     1021 gctcggcctt gtcgatcgaa acgaaacggg tttccgtctt ttgttcggcc ttctcgcgat
     1081 ccttgatcac ggtctcgaaa ctctcataga cctcggccga cagcaggtcc ttcagcgcgc
     1141 gacgatcgcc gttggcgaac gccagcacaa tcatctcata ggcgctgcgc gcgccgctaa
     1201 ggaaatgtcg cggatcaaac gaggaatcct gcgcggcgat ggcatcgagc ccctgtgcga
     1261 gcggcgtgcc gggctcggcc aggcccttcc agcgatcgct gggcggcacc acatccggcg
     1321 tcggcgccag tggcgcctgg tcgatcaccg cgcccggaat cggaaccaca ttattgtcct
     1381 gcgccccctg caccatgttg cgcgccgcgc catcgtaggg tggccgctcg tttccggtgc
     1441 gctgtccgag gacattacgc agacgcagga aaataaagac cgccagcgcc aggaagatga
     1501 tggtgtaaat atccacgtcg cattcgcttt ctggtctgcg ccggcaacgg agccggcggt
     1561 agagcgttcc ctaaaggaga acggcagaga ctacatcatg gatgggtctg aagcatgtag
     1621 gcacgaaact tggcctggcc aatggcgcgt tttggcttga aacgccatac cggtggaaat
     1681 aggccaagcc gactgctcgc tgcccgttta ctgctaacga attgtagcgc cttttggttg
     1741 cgaggggaaa cccgttactg cccggaaatc gcgcatattc aacaatttag agtgcagcct
     1801 ccgcactgga ccggcctgcc tggttgggtt ctgccgtgcc ggccgattcg aaaagcatcg
     1861 gcttggccgc acagcggcaa ttgtcatcct tgtggaatga ggcgaggcta tgatagccac
     1921 tcgccccgaa gagggcattt tcgcacctca cgagcgaatt caggcgcaaa accggtcccg
     1981 cgcgccttag aacgttccag gagagacttc catgaccaac ggcaacggcg cgccccctga
     2041 ggccgcccct cctccccagt tgaacgtgct ggcgcagtat accaaggacc tgtcgttcga
     2101 aaatccgaac gcgccagcct cgctggcgcc gcaagcgcag caacccgcga tcaatatcca
     2161 gatcaacgtc agcgccaaca atatcgccga aaatgaattc gaagtgacgc tttcggtcga
     2221 aggcaaggcg gaaaacggcg gcaaggtgat gttcagcttc gacctggcct atgccggcgt
     2281 attccgcctt ctcaacatac cgaagcaaga cctgcatccg atggtcatga ttgaatgtcc
     2341 gcggctgctg tttccgttcg cacgcgaaat catcgcgaca tcggtgcgcg acggcggttt
     2401 ccccccgctg atgctggatc cggtcgattt tgtcggtctg taccggcaga atatggaacg
     2461 gcaagctgcc gcgcaggctg cccaggccaa accgagctga accagctcgc gcctgcgcgt
     2521 taacgggtta cgcgctcagg aaatcgctcc agatcggctt gtcgcccagc gtggcgacaa
     2581 acgcccgatg cgccgcgcga tcgtcctccg tgacccgcgg cgcgagcggc gccaggcgct
     2641 gccgccgcgg catttcgccg tcccagcctg cacgcgtatc gcgggtctcc gccgccagga
     2701 tcagttgcga ctgccgcgcc ccaatcaggt cgatgtagac ttcggccaga agttcggcgt
     2761 cgagcaacgc gccgtgcttg gtgcggcggg aattgtcgat ggcatagcgc gagcagagat
     2821 catccagacg gttcgaaacg ccgggatgtt tgcgtcgcgc cagcagaagt gtgtcgacca
     2881 gccggtcgcg cgcgatcgac ggccgcttga tgcgatcaag ctcggcgttg atgaagccga
     2941 tatcgaatga ggcgttgtgg atcaccagcg gcgcgtcggc gatgaattcc aggaagtcat
     3001 cgaccacctg cgcgaacagc ggcttatcgg cgaggaactc gccggaaaga ccatgcaccg
     3061 cgaaggcctc agccggcatg tcgcgttccg gattgagatg ccggtgaaag gtctgccccg
     3121 tcggcatgcg attgaaaatc tcgacacagc cgatttcaac cagccgg
//
LOCUS       DASWGM010000255         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2380646,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000255 DASWGM010000000
VERSION     DASWGM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380646"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VGA15_07605"
     CDS             complement(<1..1296)
                     /locus_tag="VGA15_07605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9587595.1"
                     /translation="MAQVWTPLASAASLWASTAEPLRAFPCLSDEVQADVVIIGAGYT
                     GLSAAHHIAKSGLKPVVLEANRPGWGASGRNGGVITAKFRQSFRGIDAAHGRAMAKRM
                     YEIAHESTEMVEELVSEFGIAGARLTRSGQVKAAHNHATLRAAIDEADWMKREMGDAD
                     VRTLDAHQVREETGSQAFVGGVLNPGSGGIHPLNYLHGLAEGLARRGVAIFQESPALL
                     LQRENGLIVAETPQGAVRARQAIIATNSYSDLTPATRHMQHTLVPFRSAIIATDKLSP
                     DLAARLMPTGRTYTETKRMMRWFRMVDDRVVFGGRGAFGKQDSESAFNALRKAMIGIF
                     PELAGVPLSFKWSGLVGMTLDSMPHIGRLDDQTLFSMGYNGAGVAMSSLMGRYLAAFV
                     RGEKPDVGLLDASRLKSIPFYPLREPAVRMVAGWYQFLDA"
     gene            complement(1308..>1635)
                     /locus_tag="VGA15_07610"
     CDS             complement(1308..>1635)
                     /locus_tag="VGA15_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9587596.1"
                     /translation="CYAHALGDPFDDTVCADDTGVAKPNPEFFAYNKGRQAAFGYKQS
                     DILHVAQSQYHDIGIARKLGYQVCWIERRQGMAGFGGTPDVPALTKPDFHFATMKAFA
                     DAAVGH"
BASE COUNT          312 a          512 c          513 g          298 t
ORIGIN      
        1 cgcgtctaaa aactggtacc agccagcaac cattcggacg gccggttcgc gcagcggata
       61 aaatggtatt gatttgaggc ggctggcatc gagcaggcca acatccggct tttcgccgcg
      121 cacgaaggcc gcgaggtaac gtcccattag gctggacatc gcgacgccgg cgccattgta
      181 gcccattgag aacagagtct gatcgtcgag gcggccgata tgcggcatgg aatcgagggt
      241 catgccgacc aggcccgacc atttgaatga cagcggtaca ccggccaatt cggggaagat
      301 gccgatcatg gctttgcgta gcgcgttgaa agctgattcc gaatcctgct tgccgaaggc
      361 gccgcggccg ccgaagacca cgcgatcgtc gaccatacgg aaccagcgca tcatgcgctt
      421 ggtctcggta taggttcggc cggttggcat cagacgagct gcaagatcgg gggacagctt
      481 gtcggtcgcg atgatggcgc tgcggaacgg caccaatgta tgctgcatat gacgcgtggc
      541 cggggtcaga tcggaatagc tgttggtggc gataatcgcc tgccgtgccc gcaccgcgcc
      601 ttgaggcgtc tcggcgacga tcagcccatt ctcgcgctgt aacaggagcg caggactttc
      661 ctgaaagatg gcgacgccgc gccgcgccag cccttcagcc aggccgtgca gataattcag
      721 cggatggatg ccgccggacc ccggattgag cacgccgccg acgaacgcct gcgagccggt
      781 ttcctcgcgc acctgatgag catcgagtgt gcggacatcc gcatcgccca tctcgcgctt
      841 catccaatcg gcttcgtcga tggcggctcg aagcgtcgcg tgattgtgcg cagccttgac
      901 ctgtccgctt cgcgtcaacc gcgcgccggc aatgccaaat tcggaaacca gttcctcgac
      961 catctctgtc gattcatgcg cgatctcgta catgcgcttc gccatcgcgc ggccgtgggc
     1021 cgcgtcgata ccgcggaagg actgacggaa tttcgctgtg atgacgccgc cgttgcggcc
     1081 gctcgccccc caaccgggac gattggcctc gagcacgacc ggcttcaggc cgctcttcgc
     1141 gatatgatgc gcagccgaga gcccggtgta gcccgcgccg atgatgacaa catcggcctg
     1201 aacctcatcc gacagacaag gaaaggcccg caagggctcg gccgtgctcg cccagagcga
     1261 ggcggccgat gccaatgggg tccagacctg tgccatggcc gcgccgttca gtgcccaacc
     1321 gccgcgtcgg cgaaggcctt catcgttgcg aaatgaaaat cgggcttggt cagcgccggc
     1381 acatcgggcg ttccaccgaa tcccgccatg ccctggcggc gttcgatcca gcacacttgg
     1441 tagccgagct tacgagcgat cccgatatcg tgatactggc tctgagccac atggagaata
     1501 tcggactgct tgtagccgaa ggctgcctgc ctgcccttgt tgtaggcaaa gaattcggga
     1561 ttcggtttgg cgacgccggt atcgtcggcg caaacggtat cgtcgaacgg gtcgcccagg
     1621 gcatgcgcgt agcac
//
LOCUS       DASWGM010000256         3548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_434908,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000256 DASWGM010000000
VERSION     DASWGM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434908"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VGA15_07615"
     CDS             complement(<1..688)
                     /locus_tag="VGA15_07615"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX9587597.1"
                     /translation="MPLKTIVVAVIIAAIVLVSGLILLGLAGDFLVDWAWFSAVGYLS
                     VFWTILSTKTVIFFAVFVCSGAFLWLNGLLASRFSGRGKHAHPLPFDWQSVRGHTLAE
                     LLKLMPRHVAWPRLVAGVAGILGILIAAAQMSNWDVFLRFLYHVPYGQDDPVYGRDIG
                     FYLFSLPAYVALKNWMLLILVVCACFAGAVYWVHGDVEFDRQPPSVSPAAIAHGSALL
                     GLFFAVKAWSY"
     gene            complement(1000..1308)
                     /locus_tag="VGA15_07620"
     CDS             complement(1000..1308)
                     /locus_tag="VGA15_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587598.1"
                     /translation="MAGKKPNIDRIGISYMLRGEAGADVQDLNAKTPPEGKDWYYAGP
                     HVMLVLPDSDKDALKDVGQDTSSGAPYVRGPGSLSPLLVVPVAKPDEEIIIRTAAASK
                     "
     gene            1456..1932
                     /locus_tag="VGA15_07625"
     CDS             1456..1932
                     /locus_tag="VGA15_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9587599.1"
                     /translation="MPFETRLTAQDCNCLAVRQAARRVTQFYDRFLAPAGLRTTQFSI
                     LARLGRKGPMPINALAADMVMDRTTLGRNILPLERDGLIGIKPGAADRRSKEIRLTKA
                     GQKRLRVAFKGWMEAQAQFESAFGSARASDLRALLRAVVAIDPARAEAAVTRSSDG"
     gene            2035..3285
                     /locus_tag="VGA15_07630"
     CDS             2035..3285
                     /locus_tag="VGA15_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587600.1"
                     /translation="MSRQRNFWIPVICGGMILTIGIGARQSFGIFQKPIAADLNVGRE
                     LWSFGTALAMLLMGVFSPFVGNLADRFGTARTVAAGGMLYVAGMLMIALATEGMMLTL
                     GNVLSGIGMAAAGFGPIFGAIARQTPADKRSVALGVATAGGSFGQFAIVPLASILQSR
                     FDNWHTTMFVLTALSALMVLLAFGLRENRAAGFGPLGTARTQSTQNALQEAFRTQGFW
                     LLTIGFFVCGFHVSFIGLHLPSYISDNAIGMAFFGRPISAIELGGWAIGLVGLFNIAG
                     SLLWGWLGGHHLRKDMLALLYALRALAFVVFLALPLSWVSVLLFAASLGFLWLGTVPL
                     TSGLVGYMFGPAHMSMLWGVVFFSHQLGSFLGGWGAGRLYDIQGNYNSMWWISVGLGV
                     FAALIHWRIREQPVERLALGVAPS"
BASE COUNT          621 a         1088 c         1132 g          707 t
ORIGIN      
        1 cataggacca ggccttcacc gcgaagaaca gacctaacag ggcggagccg tgggcgatgg
       61 ccgccgggga caccgaaggc ggctggcggt cgaattcgac gtcgccgtgg acccaataca
      121 cggctccggc gaagcaagcg cacacgacca ggatcagcag catccaattc ttgagggcga
      181 cataggcggg aagcgagaag aggtagaagc cgatgtccct gccgtacacg ggatcgtcct
      241 gtccgtacgg cacgtggtag aggaaccgca ggaatacgtc ccaattgctc atctgcgccg
      301 cggcaatcag gatcccgagg atgccggcga ccccggcgac gagtcgaggc cacgccacat
      361 gccggggcat gagcttgagc agctcggcca atgtgtgtcc cctgacggat tgccagtcga
      421 agggtagggg atgcgcatgc ttgccgcggc cggaaaaccg ggacgccagc aacccgttca
      481 gccacaggaa agcgccggag cacacgaaga cggcaaagaa gatgacggtc ttggtgctga
      541 gaatcgtcca aaacacgctc agatacccga ccgccgaaaa ccaggcccaa tcgaccagga
      601 aatcaccggc gagtcccagc aggatcaggc cggaaaccaa gacgatggca gcgatgatga
      661 ccgccaccac gatggtcttc aatggcatgc ccgcctccga ccggccgcgc gtgacgatcg
      721 caacttcggc cgctgccgat ccgcgtgaag caggtgtatc ggcagcagac tgggatccgg
      781 cccggttgat attagctcga tctggccttt tttagaacac gcgtcggcaa ggtcggctcg
      841 ggatcatcct gagaccctcc gaccacgtcg gcggcacagc ccgctctgcc tcaactgcgg
      901 aagaagccgc aagctgccgg caccgctgcg aaatgccagt agcggggcta caggtggacg
      961 attctggtct ttgtatcgag acgccaaatt ctcgcgcgct cacttcgagg cggccgccgt
     1021 tcttatgatg atctcctcgt cgggttttgc cacggggaca accaggagtg ggctgagtga
     1081 accaggcccc ctgacatatg gcgcgccgct cgaagtatcc tggcccacgt ctttcagtgc
     1141 gtccttgtcg ctatctggta ggaccagcat gacgtgtggc cccgcatagt accaatcctt
     1201 tccctccgga ggcgtcttcg cgttcagatc ctgcacatcg gcgccggctt ctcctcgaag
     1261 catgtacgat atcccgatcc tgtcgatgtt gggttttttg ccagccatat ttgccgaggc
     1321 gcgaagacgt gacgcggccg cgccgatcaa tcgaccgatg caagcccggt tttgctgcac
     1381 ggtattcagc gcatatgcgc ctgttgacat cgacgcatat acgcgtatat gcactcttgt
     1441 cggaggattc cagcgatgcc tttcgaaact cggttgacgg cgcaagactg caattgcctg
     1501 gcggtccgtc aggcggcgcg tcgcgtcacc caattctatg accggttcct ggcgccggcc
     1561 gggctgcgta ccacccagtt ctcgattctt gcgcggctgg gacggaaagg tccgatgccg
     1621 atcaatgcgc tcgccgccga catggtcatg gaccgcacca cgctcggacg gaacatcctg
     1681 ccgctggagc gcgacgggtt gatcgggatc aagccgggcg ccgccgaccg ccgcagcaag
     1741 gaaatccgcc tgaccaaagc cggccaaaag cggctccggg tagcgttcaa aggatggatg
     1801 gaagcgcaag cccagttcga atccgctttc ggcagcgccc gcgcgtcgga cttgcgcgcg
     1861 ctgctgcgcg ccgtggtcgc cattgatccc gcccgcgcgg aagccgccgt cacccgatca
     1921 tcggacggat aggaaagcca ttgccctgtt tgggaaggcc ccatgtctgc ctggtcgcac
     1981 ttctcgggtc ggaccaacgc tgcatcgaag caccgcggct ccgaaaggat tgccatgtca
     2041 cgtcaacgta acttctggat tcccgtgatt tgcgggggca tgattctgac catcggaatt
     2101 ggcgcgcgcc agagcttcgg gatttttcag aagccgatcg ccgcggatct caatgtcggc
     2161 cgcgagttgt ggtcattcgg caccgcgctt gccatgttgc tgatgggtgt gttctcgccg
     2221 ttcgtgggca acctggccga tcgtttcggc accgcgcgca cggttgcggc cggcggcatg
     2281 ctctatgtgg ccgggatgct gatgattgcg ctggcgaccg agggcatgat gctgaccctc
     2341 ggcaacgtgc tctccggcat cggcatggcg gccgcggggt tcggcccgat cttcggagcg
     2401 atcgcgcggc aaacgccggc cgacaagcga tcggtcgcgc tgggcgtcgc gacggcgggc
     2461 ggctcgttcg gccagttcgc catcgtgccg ctcgcttcga tcctccagag ccgcttcgac
     2521 aattggcata cgacgatgtt cgtgcttacc gcgctttcgg cattgatggt ccttctggcg
     2581 ttcggcttgc gcgaaaatcg tgcggcaggt ttcggccctc tcggtacggc gcgcacgcaa
     2641 agcacccaga atgcgctcca ggaggcgttt cgcacccagg ggttctggct gctcaccatc
     2701 ggcttcttcg tatgcggctt ccacgtgtcg ttcatcggct tgcaccttcc ctcctatatc
     2761 tccgacaatg ccatcggcat ggccttcttc ggacgtccga tatccgcgat cgaactcggc
     2821 ggatgggcga tcggccttgt cgggctcttc aacatcgccg gatcgctgct gtggggatgg
     2881 ctcggcgggc accatctgcg caaagacatg ctggcgctgc tctatgcatt gcgcgccctg
     2941 gcgttcgtgg tgtttcttgc gctgccgctg tcttgggtgt ccgtgctgct gtttgcagcg
     3001 tcgctcgggt tcctgtggct gggcacggtt cccctgacca gcgggttggt cggctatatg
     3061 ttcgggccgg cacacatgtc gatgctgtgg ggtgtggttt ttttcagcca ccaactgggg
     3121 agtttcctgg gcggctgggg ggccggacga ctctacgata ttcagggcaa ctacaattcg
     3181 atgtggtgga tatcggtcgg gctcggggtg tttgcggcgc tgattcactg gcgtattcgc
     3241 gaacaaccgg ttgagcggct cgcactggga gtagcgccgt catgaatcgc ggcagcacgg
     3301 accgtaccga tggtccggcg gcgccggatg caggcgatcc cgcatgggtg cctctagcgg
     3361 agccattgcc gcgcaaccgg ccccgttggc tgctctgggc ggggggtgca ttggctctcg
     3421 caatatgtct cgcgatcttg ctgatgtggg gaattctcgg tcctgcatat atttttgatc
     3481 taattgccgc gtactgcgcc tagagcggaa tgaacttcag caggccgtgg catggcgacg
     3541 ggtcgcac
//
LOCUS       DASWGM010000257        11330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2241760,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000257 DASWGM010000000
VERSION     DASWGM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11330
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241760"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..394
                     /gene="crcB"
                     /locus_tag="VGA15_07635"
     CDS             8..394
                     /gene="crcB"
                     /locus_tag="VGA15_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX9587601.1"
                     /translation="MSVALGSVVGGVARYLVSVLFASQLGDGFPWSTLFVNVTGSFII
                     GFYSALTGPDGRLLASPRQRQFVMVGICGGYTTFSAFSLETLRLVQSGNLHAALLNIG
                     ISVVGWLTAVWIGHALATRLNRLRGT"
     gene            397..738
                     /locus_tag="VGA15_07640"
     CDS             397..738
                     /locus_tag="VGA15_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX9587602.1"
                     /translation="MQIPKDAMLLRIFIGESDQFGDRPLHEAIVLKAREMHLAGATVL
                     RGPMGFGQSSLLHTAKILRLSVDLPLIIEIVDGEEKIRELLPALEEMIGSGLVTLEKV
                     QVLQYGKNKND"
     regulatory      854..914
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            1099..2346
                     /locus_tag="VGA15_07645"
     CDS             1099..2346
                     /locus_tag="VGA15_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9587603.1"
                     /translation="MTSVWALAALWLGLALVASLLSIWFRIATALSEIVVGTIAQLII
                     GAAIGSAVLGTDESWVKFLSGLGAIVLTFLAGAELDPQVFKLKWKEAASVGLASFFFP
                     FFGCTAAAYYLLGWELMPSWLAGVAMSTTSVAVVYAVMLEFGFNTTDYGKIVLAACFV
                     TDLGTVVALGLIFAPFTMKTLVFLSAGIVVFVVLPWVTPRFFRLYGNRPSELEAKFLL
                     LFLLGMGALATWADSEAVLPAYLIGMVLAGTVGKNHALIRRLRTLTFGLLTPFYFIRA
                     GSFVSIPDLVAAPAAFVLFLLVKVGTKIVGVYPVTKLFGAPNQEAIYTTLLMSTGLTF
                     GTISALFGLSHGIISQGQYSALVAAVIASAVVPTVIANAFYLPRHLLPKQHAKELEAE
                     PALMSAFVDIRKNTGSGRCSEGS"
     gene            2328..2747
                     /locus_tag="VGA15_07650"
     CDS             2328..2747
                     /locus_tag="VGA15_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9587604.1"
                     /translation="MFRRILSANDGSGPAFKALALALDLAKQNNSELHMASVEEVVSF
                     PELIGEVENERHAADRRYRNVLKRAKAMAAEKDVRLETHVLVGHPVRSIVDLASELGA
                     DLLVIGATGHSALYERMLGSRADRVIQLASCPVLVVK"
     gene            2831..3172
                     /locus_tag="VGA15_07655"
     CDS             2831..3172
                     /locus_tag="VGA15_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEX9587605.1"
                     /translation="MQIPREAVLLRIFFGENDRFQRLPLHEAIVLKAREMHLGGATVL
                     RGHIGFGHSSRIHTTKILRLSQDLPVVVEIVDSQEKIDTFLPVLDGMMSSGLITIERA
                     EVLQYGGESKL"
     gene            complement(3314..4882)
                     /locus_tag="VGA15_07660"
     CDS             complement(3314..4882)
                     /locus_tag="VGA15_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587606.1"
                     /translation="MTARLTIFLFGTLLAGTAGAAAGDKTDVVRDLAGRVGPIVGSAS
                     ACRDIARPRVQLIADKFAGVIAEASSNEAERSDLTHLFERSVADGRNAVTTGRIDCRL
                     ADRQLADIERSIAGSAAPNSLAAVIAPSSAAAATSPTTPATPVAGGPPVRGVTDQEIR
                     FGMAAPFSGAAKELGRQMKLGIDTAFNRINEAGGVEGRMLKLITADDGYEPTRTGDAM
                     KQLYEKDQVFGIIGNVGTPTAVVAVPYALERRMLFFGAFTGANVLRHDPPDRYVFNYR
                     ASYAEETDATVRYLVKLRRIPIRQIAVFAQQDTYGDSGFAGVAKAVRTLGVSDSTIVR
                     LNYKRNTVDVDDAVNQLKLQKPPIKAVVMVATYRAAAKFIEKTHDLFPGMLYTNVSFV
                     GSTALADELMLLGPRYATGVIVTQVVPAVSGYSSVVLEYKNALAKYFAGESPDYVSLE
                     GYVAANVLIKGLQRAGPQLDTEKLIDALENSRTFDLGLGANLGFGRAEHQASHKIWGT
                     AIDENGKYQAIELE"
     gene            complement(5022..6599)
                     /locus_tag="VGA15_07665"
     CDS             complement(5022..6599)
                     /locus_tag="VGA15_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587607.1"
                     /translation="MNLRWTILLVSVLLAGAAEAAPGDKTDIVRDLAGRVGPMVGSAL
                     ACRDIARPRIQVIVDKFTAVITQFSSSEAERADLTQLLDRNITDGRTAVTSGRIDCRL
                     AERQLADLEQSIARPPLPAAAPPPSATVAPSFAAAAATAPTAPVAAVAGPPVRGITDQ
                     EIRFGISAAFTGAAKELGRQMKLGIDTAFNRVNEAGGVEGRMLKLIAADDGNEPKRTA
                     ETMKQLYEKDQVFGIIGNVGTPTAAVAVPYALERRMLFYGAFTGANVVRHDPPDRYVF
                     NFRPSYAEETDAAVRYLVKLRRIPPNQIAVFAQQDPYGDAGFAGVAKAFRALGVDDSG
                     ILRLNYQRNTVDVDDAVNQLKLQKPPIKAVVMAAVYRAAAKFIEKTHDFSPGMIYTNV
                     SGVGGTALADELMLLGPRYATGAIVTQVVPAVSGYSSVVLEYKNALAKYFPGEAPDYV
                     SLEGFVSANILIQALKRAGPQIDTEKLIDVLENMRNLDLGLGVPLNFGRAEHQASHKI
                     WGTAIDENGKYQAIELE"
     gene            complement(6715..8280)
                     /locus_tag="VGA15_07670"
     CDS             complement(6715..8280)
                     /locus_tag="VGA15_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587608.1"
                     /translation="MSLRWMMVVSGALLAGTGSVSAGDKTYIVRDLAGRVGPIIGSAL
                     ACRDIARPRIQVIVDKFTAVITENSSNEAERSDLTLLLDRNVADGRSAVTTGRIDCVS
                     ADRQLADLERSIVPRSAVPAPSTSSIMAPSSAVAATAPTAPVSVAPPVRGITDREIKF
                     GIAAAFTGPTKELGRNMKLGIDTAFNRTNGAGGVEGRMLKLVSSDDGNDPARTLDAMK
                     QLYDKEQIFGIIGNTGTPTGAIAVPYALERRMLFFGAFTGANVVRHDPPDRYVFNYRP
                     SYAEETDAAVRYLVKLRRIPVRQIAVFAQQDGFGDSGFAGVAKAFRALGVDDSGILRL
                     NYQRNTVDVDDAINQLKAQRVPTKAVVMVATYRVAAKFIEKTHDLFPGMIYTDVSAVG
                     GTALADELMLLGPRYATGAIVTQVVPAVSGYSSIVLEYKTALAKYFPGESPDYVSLEG
                     FISANILIQALKRAGPQLDTEKLIDALENMRNIDLGLGVPLNFGRAEHQASHKIWGTA
                     LDESGKFQAIELE"
     gene            complement(8411..9973)
                     /locus_tag="VGA15_07675"
     CDS             complement(8411..9973)
                     /locus_tag="VGA15_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587609.1"
                     /translation="MTLRWMTVVFGALLAGAGGAAAGDKTDVVRDLAGRVGPIVGSAL
                     ACRDISRPRIQVIVDKFTAVITEASSSEAERSDLTQLLDRSVADGRSAVTSGRIDCRV
                     ADRQLGDLERSIAGPGPSLAAAIAPSFAAAAATAPTAPVAAVAGPPVRGVTDREIRFG
                     IAGPFSGSSKELGRQMKLGIDAAFNQANDAGGVEGRMLKLIAADDGFEPTRTAEAMKQ
                     LYEKDQVFGLIGNVGTATAAVAVPYALERRMLFFGGFTGANVIRHDPPDRYVFNYRAS
                     YAEETDAAVRYFVKMRRIPPRQIAVFAQHDAGGDAGFAGVAKSFRSLGFSDSGILRLN
                     YERNTVDVEDAVNQLKAQKVPIKAVVMISAYRPAARFIEKTRDLFPGMLYSNISFVGA
                     TVLADELMLLGPRYAAGAIVTQVVPSVSGYSSIVLEYKTAIAKYFPGEAPDYVSLEGY
                     VSANILIQALKGCGPQLDTEKLIDVLENTRQLDLGLGTQLGFGRAEHQASHKIWGTAL
                     DESGKYQAIELE"
     gene            complement(10054..10677)
                     /locus_tag="VGA15_07680"
     CDS             complement(10054..10677)
                     /locus_tag="VGA15_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587610.1"
                     /translation="MSSLSEQDPLNPNDQLYYAPRWLRERSEPVPPLPSAVPSPSAEK
                     KSESLARPIPNSPPHSFDALLEEAVAESLRHPLDPQVMNEPPGFVRELDRRMAILSVA
                     GRFAAAIGVSAIVALFFVIMVPASRDYSRQPETDNSSVSGFLQSLKTALSQPRQKDDD
                     AKPALAEFQAILASPPAAPPPVSREESETLLQQFVQWQQKPPQPTAP"
BASE COUNT         2213 a         3308 c         3417 g         2392 t
ORIGIN      
        1 ttcttatatt tccgtcgcct tgggcagcgt agtcggcggc gtggcgcgct atctggtttc
       61 tgttctgttc gcgagccagc tcggcgacgg ctttccctgg agcacattgt tcgtcaacgt
      121 gacgggctcc ttcatcatcg gattttattc tgcattgacg ggccccgatg gccggttgct
      181 cgcgagcccg cggcagaggc agttcgtgat ggtcggaatt tgcgggggct acaccacttt
      241 ttccgcgttc agtctcgaga cgttgcgact tgtgcaatcc ggcaatttgc acgccgcatt
      301 gctcaatata ggcatctccg tcgtgggctg gttgacggcc gtgtggatcg gtcacgccct
      361 tgcgacgcgt ctcaatcgtt tgcgagggac ctgaaaatgc aaataccaaa agatgcaatg
      421 ttgctgcgaa tttttatcgg cgagagcgat caattcggcg atcggccgct ccatgaagcc
      481 atcgtgttga aggcacgcga gatgcatctg gccggcgcca cggtgttgcg tggaccgatg
      541 ggcttcggcc agtcgagcct gttgcacacc gcaaagatcc tgcgcctctc ggtggacttg
      601 cccttgatca ttgaaatcgt ggatggcgag gaaaagatcc gggaattgct gcctgcgctc
      661 gaagagatga tcggcagcgg gctcgttacg ctcgagaaag tccaggtgct gcaatacggc
      721 aagaacaaaa acgattgatg cgtggggacg aacgcgaggc tccggaaaac gcgcgaatgc
      781 aatttaattg cgggtcgagc cctggaaaca gttgatcctt gttttcgtcg atttctcggc
      841 taagatccaa acatccaaaa acggggatgg ggtcccccaa aaccgccgaa aaggctgatg
      901 actcctgctg cgccaccgca agcaggagtg tgtcttgaac ctacctgaac gagcggcttt
      961 ttttcgaatt ctcgcctgtt tttccgggcg acacatcagt cgctcgcccg gtccgcgccg
     1021 cgacgagttc gcatggcctc tgccttggtt tttgttcggt ctgatcgggg cactccttga
     1081 gcggcaggat catgcgccat gacaagcgta tgggcattgg cagcactgtg gctcgggctc
     1141 gcgttggttg ccagcctgct ctcgatctgg tttcgcatcg cgacggcatt gtcggaaatc
     1201 gtcgttggaa cgattgcgca gttgatcatc ggagccgcga ttggctcggc cgtactcggc
     1261 accgacgaat cctgggtcaa gtttttatcc ggccttggcg cgatcgtact gacgtttctg
     1321 gcgggtgcgg aactcgatcc gcaggtgttc aagctcaaat ggaaggaggc ggcatcggtc
     1381 ggacttgcca gcttcttctt tccgtttttt ggctgcacgg cggctgccta ctatctgctc
     1441 ggatgggagc tgatgccgag ctggcttgcc ggcgttgcga tgtccaccac ttccgtcgcg
     1501 gtcgtctatg ccgtgatgtt ggagttcgga tttaatacca cggactatgg caagatcgtt
     1561 ttggcggctt gtttcgtcac cgatctcgga acggtcgtcg cactcggctt gatcttcgct
     1621 ccgttcacga tgaagacact ggttttcctc agcgcgggga ttgtggtctt tgtcgtgctt
     1681 ccatgggtca cgccccggtt ttttcggctt tacggcaatc gcccttctga actggaagca
     1741 aaattcctgc tgctgttcct gctgggaatg ggcgcattgg cgacctgggc cgatagtgag
     1801 gcggtgcttc cggcctacct gatcgggatg gtgcttgcgg gcacggtcgg caagaaccac
     1861 gcgctgatca gacggctgcg gacgcttaca ttcggactgc tgacgccgtt ctattttatt
     1921 cgcgcgggct cctttgtttc catacccgat ctcgtcgccg cgcccgccgc cttcgttctt
     1981 tttcttctgg tgaaggtcgg aaccaagatc gtcggcgtgt atcccgttac caaattattc
     2041 ggggcgccca accaggaggc catctacacg accctgctga tgtccaccgg cctcaccttc
     2101 gggactattt cggctctgtt tggcctttcg catggaatca tcagtcaggg acaatattcg
     2161 gcgctggtcg ccgccgtgat tgccagcgcg gtggtaccga cggttatcgc caacgccttc
     2221 tatctgccgc gtcatctctt gccaaagcag catgcaaaag agctcgaagc ggaacctgcc
     2281 ttaatgtccg cctttgtgga catcaggaaa aacaccggga gtggccgatg ttcagaagga
     2341 tcctaagcgc gaacgatggt tccggccctg ccttcaaagc gcttgcgcta gcgctcgatc
     2401 tggccaagca aaacaactcc gaattgcata tggcgtcggt cgaagaggtg gtgtcgttcc
     2461 cggaattaat cggagaggtc gaaaacgagc ggcacgcagc cgatcgtcgt tacaggaatg
     2521 tgctgaagcg cgccaaggca atggccgcgg aaaaggatgt gaggctggag acccatgtgc
     2581 ttgtcggtca ccccgtgcga agcatcgtcg atctcgccag tgaactcggt gctgatctcc
     2641 tggtgatcgg ggctaccggc cattcggcgc tgtatgagcg aatgctcgga agtcgcgccg
     2701 acagagtcat tcagttggct tcctgcccgg ttctggtcgt aaaatagcat ggcgcgttca
     2761 tggatattcc agacgggagc gggatcaggt tagccggcca gttgtagttc aaaaaaatgg
     2821 gggagggatc atgcaaattc cgcgggaggc cgtcctgctc cgcatctttt tcggagagaa
     2881 cgacaggttt caacgccttc ctcttcatga ggcgattgtc ctgaaggcgc gggagatgca
     2941 cctcggtggc gcaaccgtcc tgcgcggcca tatcggattc ggccattcga gccgcattca
     3001 tacaacgaag atcttgcgcc tctcgcagga tcttcctgtc gtggttgaga tcgtcgactc
     3061 gcaagaaaag atcgacactt tcttgcccgt actcgatggc atgatgtcga gcggtctcat
     3121 caccatcgag cgagccgaag ttcttcaata tggaggcgag tccaagctgt agcccaagga
     3181 ataagccgat gaccggcgtc ccgtgcgtct cgcatgctgg ttcatgcccg ccacagacaa
     3241 cccttcctgg gatgcccgac gtggtatcgc ggcaggatca ggagaacggc tggccctgcc
     3301 gccgagggcg atatcattca agttcgatgg cctggtactt cccgttttcg tcgatcgccg
     3361 ttccccagat cttgtgcgaa gcttggtgtt cggcgcggcc gaaaccgaga ttggcgccaa
     3421 gacccaggtc gaaagtgcgc gagttctcca gggcgtcgat cagcttctcg gtatcgagtt
     3481 gcggtccggc acgttgcagc cccttgatca gaacgttggc cgcaacgtag ccctcaagcg
     3541 acacgtaatc cggtgactcg ccggcaaagt atttggcaag ggcattcttg tattcgagaa
     3601 ccacactcga ataaccggaa accgccggca ccacttgcgt cacgatgacg ccggtagcat
     3661 agcgcggtcc caacagcatc aattcatcgg caagcgcggt gctgcccacg aacgacacat
     3721 tggtgtaaag catcccagga aacaggtcgt gcgtcttctc gatgaacttg gcggcggccc
     3781 gataggtggc caccatgacc accgctttga tgggcggctt ctgcagcttc aactgattga
     3841 ccgcgtcgtc cacatccact gtgtttcgct tgtagttgag ccgcacaatt gtgctgtcac
     3901 tgacgccgag cgtgcgtacg gccttcgcga caccggcgaa gcccgaatcg ccgtaagtat
     3961 cctgctgggc gaaaacggcg atctgcctga tcggtatccg gcgcaatttc accaaatagc
     4021 gcacggtggc gtcggtttcc tcggcgtagc tcgcacgata gttgaataca tagcgatcgg
     4081 gaggatcgtg gcggagcaca ttggcgcccg tgaaagcgcc gaagaacaac atccgtcgct
     4141 ccagcgcata ggggacggcg accaccgcgg tgggcgttcc gacatttccg atgatgccga
     4201 atacctggtc tttctcgtag agctgcttca tggcatcgcc ggtgcgcgtc ggctcataac
     4261 cgtcatcagc cgtgatcagc ttgagcatcc ggccttcgac gccgccggcc tcgttgatcc
     4321 gattgaaagc agtatcgatg ccgagcttca tctggcgccc gagctccttg gccgcgccgg
     4381 aaaacggcgc cgccattccg aaccggattt cttgatcggt tacgccgcgg accgggggcc
     4441 cgccggcgac cggtgtggcc ggtgtggtag gtgaagtagc ggcggcggcg gaagacggtg
     4501 caataacagc cgccaaactg tttggcgcgg cggacccggc gatcgagcgt tcgatatcag
     4561 cgagctggcg gtccgccagc ctgcaatcga tcctgcccgt cgtcacggcg ttacgaccgt
     4621 cagcgacact gcgctcgaat aggtgtgtaa ggtcggagcg ctcagcctcg ttggacgagg
     4681 cttcggcgat cacaccggcg aacttgtcgg caatgagctg aacacggggc cgcgcgatat
     4741 cccggcaggc cgacgccgaa ccgacaatcg gtccgacgcg accggcaagg tcgcgtacaa
     4801 cgtcggtctt gtcaccggca gctgcgcccg cggtgcccgc gagcagtgta ccaaacaaaa
     4861 agatagtcaa acgagcggtc atcgcaaaac ctccgcgagg tcaaagtgca aaattgccat
     4921 tgtgtcgatc agttcttgtg actttctaat caaaacgtat ccgtcgtcgg tatggcgatc
     4981 gcccgttctg ggcgacggcc ccgatattat tcaagttcat attattcaag ttcaatggct
     5041 tggtactttc cgttttcatc aatcgccgtt ccccagatct tgtgcgaagc ttggtgttcg
     5101 gcgcggccaa aattaagtgg gactccaagg cccagatcga gattgcgcat gttctccagg
     5161 acgtcgatga gtttctcggt atcgatctgc ggccccgcac gcttgagcgc ctggatcagg
     5221 atattggccg aaacgaaacc ttcaagcgac acgtagtcgg gcgcctcgcc gggaaaatat
     5281 ttggctaaag catttttgta ttcgagaacc acactcgaat aaccggatac cgccggcacc
     5341 acttgggtca cgattgcgcc ggtagcatag cgcggtccca gcagcatcaa ttcatcggcg
     5401 agcgcggtgc cgccgacgcc cgacacgttg gtgtaaatca ttccaggaga gaagtcgtgc
     5461 gtcttctcga tgaacttggc cgcggcccga tagactgcgg ccatgaccac cgctttgatt
     5521 ggcggcttct gcagtttcaa ctgattgacc gcgtcgtcca catcgacggt gttgcgctga
     5581 taattgagcc gcagaatacc gctgtcatcg acgccgagcg cgcgaaacgc ctttgcgaca
     5641 cctgcaaatc ccgcatcgcc gtaggggtcc tgctgggcga aaacggcgat ctggttgggt
     5701 ggtatccggc gcagtttcac caaatagcgc accgcggcgt cggtttcctc ggcatagctc
     5761 ggacggaagt tgaatacgta gcgatcggga ggatcgtggc gcacgacatt ggcgcccgtg
     5821 aaggcgccgt agaacagcat ccgccgctcc agcgcgtacg gcaccgcgac ggctgcggtg
     5881 ggcgtgccga catttccgat gatgccgaag acctggtctt tctcgtagag ctgcttcatg
     5941 gtctcggcgg tccgcttcgg ctcattgccg tcgtcggcgg cgatcagctt gagcatccgg
     6001 ccttccacgc cgccggcctc gttgacccgg ttgaaagcag tatcgatccc gagcttcatc
     6061 tggcgcccga gctccttggc cgcgccggta aaggctgccg atatgccgaa ccggatttcc
     6121 tgatcggtta tgccgcggac cggggggccg gcgacggcgg cgaccggtgc ggttggcgcg
     6181 gtggcggcag cggctgcgaa ggacggtgcg acggtggcag acggtggtgg ggcggcagcc
     6241 ggcaaaggcg gccgggcgat cgactgctcg aggtcagcga gttggcgttc cgccagtctg
     6301 caatctatcc gccccgacgt cacggcggta cggccgtccg tgatattgcg atcgagcagt
     6361 tgcgtcaggt ccgcgcgctc ggcctcgctt gaggagaatt gggtgatgac tgccgtgaac
     6421 ttgtcgacga tgacctggat acggggccgt gcaatgtccc ggcaggccaa cgccgaaccg
     6481 accatcggcc ccacccggcc cgcaagatcg cgtacaatat cggtcttgtc gcccggcgct
     6541 gcttccgcgg cgcctgcgag caagacgcta actaatagga tcgtccaacg aaggttcacc
     6601 gtataattcc gtgagtttaa ggagtcgaag atttctaaga gagagcatga tctgcacggc
     6661 gttggccctt tctaggcgat tgcaccaata ttattctatt gttctatggt tctatcattc
     6721 tagttcgatg gcctgaaact tgccgctttc atccagcgcc gttccccaga tcttgtgcga
     6781 agcctggtgt tcggcgcggc caaaattaag tgggactccg aggcccagat cgatattgcg
     6841 catgttctcc agcgcgtcga tgagcttctc ggtatcgagc tgcggtcccg cacgcttgag
     6901 cgcctggatc aggatattgg ccgaaatgaa gccttcgagc gacacgtagt cgggcgactc
     6961 gccgggaaaa tatttggcca aagcagtctt gtattcgaga acgatgctcg aataaccgga
     7021 taccgccggc accacttggg tcacgatcgc gccggtagca tagcgcggcc ccaacagcat
     7081 caattcatcg gcgagcgcgg tgccgccgac ggccgacacg tcggtgtaga tcattccggg
     7141 aaacaggtcg tgcgtcttct cgatgaactt ggcggcaacc cgataggttg caaccatgac
     7201 caccgccttg gtgggcactc tttgcgcctt caattgattg atcgcatcgt cgacgtccac
     7261 ggtgttgcgc tgatagttga gccgcagaat accgctgtcg tcgacgccga gcgcgcgaaa
     7321 cgcttttgcg acacccgcga accccgaatc gccgaacccg tcctgctgcg cgaacacggc
     7381 gatttgcctg accggtatcc ggcgcaattt caccaggtaa cgcaccgcgg cgtcggtttc
     7441 ctcggcatag ctcggacggt agttgaacac ataacgatcg ggaggatcat gccgaacgac
     7501 attggcgccc gtgaaggcgc cgaagaacag catccgccgc tccagcgcgt acggcaccgc
     7561 gattgccccc gtgggcgtac cggtattccc gatgatgccg aagatctgtt ctttgtcgta
     7621 gagctgcttc atggcatcga gggtgcgcgc cggatcattg ccgtcgtcgg aggagactag
     7681 cttgagcatg cggccttcga cgccgccggc cccgttggtc cggttgaatg cggtatcgat
     7741 cccgagcttc atattgcgac caagctcttt tgtcgggccg gtaaaggccg ccgctatgcc
     7801 gaacttgatt tcccgatcgg ttatgccgcg gaccgggggc gcaacggaca ccggtgcggt
     7861 cggcgcggtg gcggcgaccg ccgacgatgg cgccatgatg gaggatgtgg atggcgcggg
     7921 taccgccgac cgggggacga tcgaccgctc gagatcggcg agctgacggt ctgccgatac
     7981 gcagtcaatc cttcccgttg tcacggcgct gcggccgtcc gcgacattgc gatcgagcaa
     8041 cagcgtaagg tcggaacgct cagcctcgtt ggacgagttt tccgtgatca cggctgtgaa
     8101 cttatcgacg atgacctgga tacgaggccg cgcgatgtcc cggcaggcca acgccgaacc
     8161 gataatcggt ccgacgcggc ccgcaagatc gcgcacaatg taggtcttgt cgccagccga
     8221 aacgctcccg gtaccagcaa gcaaggcacc ggatacaacc atcatccaac gaaggctcat
     8281 cgcaaagctc ccaaaaccaa ggtgcttaag cgctgctggc gattagcccc tgtgacattt
     8341 cttgttgcaa aacttatttg taacacgtcg tcagcacggc catcgcccgt tccgggcgat
     8401 ggccgtgcta tcattcaagt tcgatggcct ggtacttgcc gctttcatcg agcgccgttc
     8461 cccagatctt gtgcgaagcc tggtgttcag cgcggccgaa accgagttgg gttccaagac
     8521 ccagatcgag ctgccgggta ttctccagga cgtcgatgag cttctcggta tcgagttgcg
     8581 gtccgcaacc cttgagtgcc tggatcagga tgtttgccga aacatagccc tcgagcgaca
     8641 cgtaatccgg tgcctcgccg ggaaaatatt tggcaatggc agtcttgtat tcgagaacaa
     8701 tgctcgaata gccggaaacc gacggcacga cctgcgtcac gattgcgccg gcggcatagc
     8761 gcggtcccaa cagcatcaat tcatccgcaa ggacggtggc gccgacgaac gatatgttgc
     8821 tgtagagcat tccaggaaac aggtcgcgcg tcttctcgat gaacctcgcg gcgggccgat
     8881 aggctgaaat catgaccacg gccttgattg gcaccttctg cgccttcagc tgattgacag
     8941 catcttcgac gtccacggtg tttcgctcgt agttgagccg cagaatgccg ctgtcgctga
     9001 agccgagcga acgaaacgac tttgcaacac ctgcgaaccc cgcatcgccg cccgcatcat
     9061 gctgggcgaa aacggcgatc tgccggggtg ggatccggcg cattttgaca aaatagcgta
     9121 ccgcagcgtc ggtttcctcc gcgtagcttg cccgatagtt gaatacatag cgatcgggag
     9181 gatcatggcg gatcacattc gcgcccgtga agccgccgaa gaacagcatc cgccgctcca
     9241 gcgcgtacgg caccgcaacg gccgcggtcg ccgttccgac attcccaata agaccgaata
     9301 cctggtcttt ctcgtagagc tgcttcatgg cctcggcggt gcgggtcggc tcaaagccgt
     9361 cgtccgccgc gatcagcttg agcatccggc cttccacgcc gccggcgtcg ttggcctggt
     9421 tgaaagcagc atcgatcccg agcttcatct ggcgcccaag ctcttttgaa gagccggaaa
     9481 acggtcccgc aattccgaac cggatttctc gatcggtcac gccgcggacc ggggggccgg
     9541 cgacggcggc gaccggtgcg gtcggtgctg tggcggcggc ggcggcgaaa gacggcgcga
     9601 tcgcggcggc caaacttggt ccgggcccgg cgatcgaccg ttcgagatca ccaagttggc
     9661 ggtccgccac cctgcaatca atccggcccg acgtcacggc gctgcggcca tccgcgacac
     9721 tgcgatcgag caactgcgtc aggtcggagc gctccgcctc gctggacgag gcttccgtga
     9781 tcacagcggt gaacttatcg acgatgacct gaatacgggg ccgtgagatg tcccggcagg
     9841 ccaacgccga accaacgatc ggaccgacgc ggcccgcaag atcgcgcacc acgtcggtct
     9901 tgtcaccagc cgcggcgccc ccggcgccag cgagcaaagc accgaacaca accgtcatcc
     9961 aacgcaaggt catcgcacgc tccgcaacat caagctttca atagacatca gacatcgtcg
    10021 tcctctcagc gaccgggcta gccgattgcg tgatcagggc gcggtgggct gcggaggttt
    10081 ttgctgccat tgcacgaact gctgaagcag cgtttccgac tcttcgcgag aaacaggcgg
    10141 cggcgcggcg gggggtgacg cgaggatcgc ctgaaactct gcaagcgcgg gttttgcgtc
    10201 gtcatctttt tgacgcggct gggatagggc ggtcttcaaa gactgcagaa acccggagac
    10261 cgacgaatta tcggtttccg gctgtcgcga atagtcccgc gaagcaggga ccatgatgac
    10321 gaagaacagc gccacgatgg ccgaaacgcc gatggctgcg gcgaagcggc cggcgacgct
    10381 gaggatcgcc attcgccgat ccagttcccg cacgaatccc ggcggctcgt tcatcacctg
    10441 ggggtcgagc ggatggcgca gcgactcggc aaccgcttcc tcgagcaaag cgtcgaaaga
    10501 atggggaggc gaattgggga tagggcgcgc gaggctctcg gatttttttt cagcggaagg
    10561 cgaaggaaca gcggaaggca aagggggcac tggttcagat cgttcacgca accagcgtgg
    10621 tgcatagtac aactgatcat tcgggttgag cgggtcctgc tcacttaggc tactcatgac
    10681 gctactctcc tcatcactct tggttacttg tttattctac cgccgtcacc cgttcggcgc
    10741 cgccgtaacg ggtctgtcgt tgttttggtt aatggacaaa tgttccaagc aatgacgtcc
    10801 gtcgcgctga aaaacccgcg atcggtccga accagacgca aagttcgatg caagatccgc
    10861 actgttcggt gtagcctgtg tcagttggcg agacatatcc ccagaccgcg tcgccaatgc
    10921 cccttccatt gccttatccc ctcggcaatt gcccgccctg aacggtagcg gaacgatctt
    10981 cggcttttag cccgaagatg tcaccggtat ttgatggaat ggtgactgga cattattagg
    11041 tccgaaactg gacgaaactg aggtaacatt ctgcggcgcc gcaattttta aagggaacgt
    11101 tagtttcttg ggcggtcgtt tttgcggttg ccgtctcagg cagcagctga ttggttccga
    11161 aatccggcgt cagcgacaaa tgcgccacaa ttgtcgccgt gactaggctg attgaacggg
    11221 ctgattcgcg gtgtcgaggt gggggtgagg tcacgaaagg cttcattaat caatcgttaa
    11281 ctaagatcgc ccatcgttaa ccattcgata agaaaggcaa gtcgaccatc
//
LOCUS       DASWGM010000258         6586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_712016,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000258 DASWGM010000000
VERSION     DASWGM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712016"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="VGA15_07685"
     CDS             complement(<1..1641)
                     /locus_tag="VGA15_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9587611.1"
                     /translation="MARKAAAGFWSQVRAGARTAWRCLGGPSWLVAGLVLIGSTLSGV
                     CQAPPVKGEAAVSVGGGYARLVLKLAEDVESEVTAAGSIIVIRFKCPVDIPVDRLYEG
                     APDYIASARRDPDGSAIRLSTARKVTINTMTAGERVFVDLLPDGWSGQLPGLPPEVVR
                     ELSERARVAERALRQQRAIADAQKRPPVRIRASVAPTFVRFVFEMPDGVGVSSVLNDQ
                     KLTLFFNAALTFDLADAKLAAPSNMASINQKIDGETSLVEVKLIGDVDVHSFREEKNY
                     IIDVGFQQTEKPSTVRLPMADASHAPAPIAAVPAAPVAAAPAAPERPAKETSPLWKVG
                     GIVPPTSETIAEQANIEIKPGSAPKAAPAPETATPARPSKEMAAQAAEAPAKNPMEVA
                     PKIAVAEPEPPPEAKAGEGAAPVDAQRDSDGLRLKFSFAAATPAALFRRADTVWLVFD
                     SIKPIDVEPIRGKGGAVIAEVSQLPLEKGQAIRIRLNRPQLPSLISVGEGGGTNWTLT
                     FADTMLAPPQPLGVIRNVTDPALANLMVPLSRPGLLHRL"
     gene            2239..2976
                     /locus_tag="VGA15_07690"
     CDS             2239..2976
                     /locus_tag="VGA15_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9587612.1"
                     /translation="MSRLSWRWWPRNKNKSLDDAELSSKRFEGGDRRHLLQALSAMVG
                     GGALAVMSQPAEARDSSDLPPPKRVLTGRDEAGKSVFKSFDVTPKVVAIDANPGLTFY
                     ELYMTEGVPRLTGLEPDPMLKGTKAFPGPGGTMFRLISYPPKRPEGWKPPPGVTFESG
                     LREMSEKVPGMGDHFERDAPGMHTSDTIDYGIVIRGEMTLELDDGQKVHLRQGDCVVQ
                     NGTRHRWRNPLPEPCLMAFISFGGKRG"
     gene            complement(3083..3511)
                     /locus_tag="VGA15_07695"
     CDS             complement(3083..3511)
                     /locus_tag="VGA15_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587613.1"
                     /translation="MVPDNVALFATIILLLPMIYFLLAAPAFLLVRLDIPPVALLLRA
                     MFNGYFLTLAVAGLIGTIAVAVAGRLVLAIGIGLIAAFAASSRRWFLRQMDARLTDRD
                     AGDADAVRRLRRLHWGGMLSNAIQLAAIVASIPYIAVAPT"
     gene            3822..3953
                     /locus_tag="VGA15_07700"
                     /pseudo
     CDS             3822..3953
                     /locus_tag="VGA15_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4107..4736)
                     /locus_tag="VGA15_07705"
     CDS             complement(4107..4736)
                     /locus_tag="VGA15_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9587614.1"
                     /translation="MSSSSNRIALFIDGANLYATAKTLGFDIDYKRLLKEFQSRGTLL
                     RAFYYTAIIEDQEFSSIRPLIDWLDYNGYTVVTKATKEFIDASGRRKVKGNMDVELAV
                     DAMELAQHIDQMVLFSGDGDFRSLVEAVQRRGVRVTVVSTISSQPPMIADELRRQADV
                     FTDLVELKTKIDRDPSERPAPREPRYQAPQFLQRATTVAPRGDDDDFDD"
     gene            complement(5556..6326)
                     /locus_tag="VGA15_07710"
     CDS             complement(5556..6326)
                     /locus_tag="VGA15_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HEX9587615.1"
                     /translation="MKSFRDIRVLPVVLIAIFSLAVLKVAGLVLDGGYVFDYQPQSSQ
                     KSWAQDTLNFPGSKFDPADITGSTHEKPNEAAAKPPAPVPEVAKPDGVVVYPEQSQPV
                     SASERAILERLQSRRQELEARAREIDIRESLLKAAEKRIDSRVEELKAVESRITTATD
                     QKTETDAARFKGIITMYEGMKPKDAAKVFDRLEMSVLFEIASQIAPRKMSDILGLMAP
                     EAAERLTIEMARRAAPDRSASAVELPKIEGKVLPQKPN"
     gene            complement(6323..>6586)
                     /locus_tag="VGA15_07715"
     CDS             complement(6323..>6586)
                     /locus_tag="VGA15_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HEX9587616.1"
                     /translation="TVRDVNENLGNQLTSAAQMSLQLKKQLAEGDNIFRRLSRIATAA
                     RPASEPEPESEPEPAVPKVSAAKAVAAAAQAFSDRRRSDGLAA"
BASE COUNT         1323 a         1963 c         1990 g         1310 t
ORIGIN      
        1 cagccggtgc agcaggcccg gtctggacaa cggcaccatc agattggcaa gcgcgggatc
       61 ggtgacgttt cggatcaccc ccagcggctg cggcggcgcc agcatcgtat cggcaaaggt
      121 cagcgtccag ttcgtgccgc ctccctcgcc gacgctgatc agcgaaggca gttgcgggcg
      181 gttgaggcga atgcggatcg cctgaccctt ctccagcggc aactgactga cctcggcgat
      241 gaccgccccg cctttgccgc ggatcggctc gacatcgatc ggctttattg aatcgaacac
      301 cagccacacg gtgtcggcgc ggcgaaacag tgccgcgggg gtcgcggcgg caaaggagaa
      361 cttcaggcgc aggccgtcgc tgtcgcgctg ggcgtcgacc ggagcggcgc cctcgccggc
      421 ttttgcttcc ggcggcggtt cgggctcggc gacggcgatt ttcggcgcga cctccatggg
      481 attctttgct ggagcctccg ccgcttgtgc ggccatctcc tttgacggac gcgcgggcgt
      541 cgctgtttcc ggcgcaggcg ctgccttcgg cgccgatccg ggcttgatct cgatgttggc
      601 ttgctcggcg atggtttccg aggtcggcgg cacgatcccg ccgaccttcc ataatggcga
      661 cgtctccttg gcgggcctct cgggtgccgc cggcgcggcg gcaaccggcg cggccggcac
      721 ggctgcgatc ggtgccgggg catgcgacgc atcggccatg ggcaaccgca ccgtcgacgg
      781 cttctcggtt tgctggaaac caacgtcgat aatgtagttt ttctcctcgc ggaaggaatg
      841 cacatcgaca tcgccgatca gcttgacttc caccaaggag gtttcaccgt cgatcttctg
      901 attgatcgat gccatgttcg acggcgccgc gagcttggcg tccgccagat cgaaggtgag
      961 cgcggcgttg aaaaacagcg tgagtttctg atcgttcagc accgacgaga cgccgacacc
     1021 gtcgggcatt tcaaatacga agcggacgaa ggtcggcgcc accgaggcgc ggatgcgaac
     1081 cgggggacgc ttctgcgcgt cggcaatcgc gcgctgctgg cgcaacgcgc gctcggcgac
     1141 ccgcgcccgt tccgacagct cgcggaccac ctcgggagga aggccgggaa gctgaccgct
     1201 ccagccatcc ggcaacagat cgacgaacac ccgttcgccg gccgtcatgg tgttgatggt
     1261 caccttgcgc gccgtcgaca ggcggatggc ggagccgtcg ggatcgcgcc gcgcggaggc
     1321 gatatagtcg ggcgccccct cgtacaaccg gtcgaccgga atatcgaccg ggcacttgaa
     1381 ccggatcacg atgatcgagc cggccgcggt cacttcggac tcgacatcct cggcgagctt
     1441 gagaaccagc cgggcgtagc cgccgccgac cgacaccgcg gcctcgccct tgaccggcgg
     1501 cgcctgacag acgcctgaca acgtcgagcc gatcaggacc agcccggcca caagccatga
     1561 cggtccaccg aggcaacgcc aggccgtccg cgcgccggcg cggacttgcg accaaaatcc
     1621 agcggctgcc ttccgcgcca ttccaaccca gactttcagt ttcaacgatc cgatcggcga
     1681 cgtgacgccc tcaccccgcg ccaaacgaat ctagaattgg cgatttaagg acctgtgaac
     1741 tttgacgagt tttaagagcc gttaagtttc gttaactaat gcgagaaact accggtttta
     1801 ttggactttt agcattatgg agtttaacaa tccgttaaga ttgataaacc gttaatcggt
     1861 agctgacatg gggtaaaacc ccgctcaggt tctggtacgg cgcaagttac cggccgttag
     1921 cgctcgcaag cctgatgccc aatcgatcca tttcgctggt tctaatcaaa gcgaatgtct
     1981 gccggatatt ggagcccttg ctatcgaagg acgggttcag tgtgctcaac ggcggcaggg
     2041 ccatctactt tcctagtcac ggtccgcaga cggaatttaa gagtcagttt ggcaccgtct
     2101 tatctggctc gccaaaatga atatcgatcg tcgctaggag ctatttcttg gacccaaaag
     2161 aatacctttg gcaattcaac aagcaccttc ccatctttat gcacgacaaa gaaagaaccg
     2221 aaacagcgga gaacatagat gagcaggctc tcttggcgct ggtggccacg aaacaagaac
     2281 aaatccttgg acgacgcgga gctctcttct aaacgatttg aaggcgggga tcggcgtcat
     2341 ctgctccagg ccctttccgc gatggtcggt ggcggcgcct tggctgtgat gtcacaacct
     2401 gctgaagcga gagactcgag cgacctgcca ccccccaaac gggtactgac cggccgcgat
     2461 gaagccggca aatcggtgtt caagtccttt gacgtaactc ctaaggtggt ggcgatcgac
     2521 gccaatccgg gactgacatt ctatgaactc tacatgactg agggtgtgcc acggctcacc
     2581 ggcttagaac cggacccgat gctcaaggga acgaaagcct ttcccggccc ggggggcacg
     2641 atgtttcgcc ttatctcgta tccgcccaag cgacctgaag gctggaagcc gccacccggc
     2701 gtcactttcg agagtgggct gagggaaatg tcggagaagg tccctggcat gggtgaccat
     2761 ttcgaacgcg acgctccagg tatgcacaca tccgatacca tcgattatgg gatcgtgatt
     2821 cgtggcgaga tgacgctgga actggacgac ggacagaagg tccatttgcg ccaaggcgat
     2881 tgcgtcgtgc aaaacggaac ccgacatcgc tggcgcaacc cgttgccgga gccctgtctc
     2941 atggccttca tctcgtttgg cggaaagcgt ggctaaggct tgggtacatt cgcggccacg
     3001 gtctgccgga tctgtcgaac aggatcggtt gagcatcacc ttcggaggaa gcattcctcc
     3061 gcaggccgtt cgtcaaacct gattaagtcg gcgcgacagc gatataagga atgctggcca
     3121 cgatagcggc aagctggatc gcattgctca gcatgccgcc ccaatgcagc cgtcgcagcc
     3181 ggcgcacggc atcagcatcg ccagcatctc tgtcagtaag ccgggcatcc atctgccgca
     3241 aaaaccagcg gcgcgacgag gccgcgaaag ccgcgatcaa gccaatgcca atggctaaaa
     3301 ccaggcgacc agccacggca acggctatcg tcccgattag accggcaacg gccaacgtca
     3361 ggaaatagcc attgaacatg gcgcgcaaca gcagggcgac aggtgggatg tccagtctca
     3421 ccagcagaaa ggcgggcgcc gccagcagga aataaatcat cggaagcagc aggatgatag
     3481 tggcgaacag ggcgacgtta tctggcacca tccgaacacc ctttttttcc cctgccgggc
     3541 aagggttttc tggcggcatt cataacaata tccgcctcga cctatcaccg acaccattgg
     3601 gctttggtcg gatgcgatat cgaaccgctc acacgaggtt tgttcaagac aaaatgaccg
     3661 cttcgggcca ttggcgaccg ggccgaacca gtgacaagtt cggccatgtc cgtatgccgc
     3721 cgaaagcgga agtaaattca gagcattagc ggttccgcca cgggccattg cgggttgatg
     3781 gggccgccgc ggacgtgatt caagctccga aaccggagcc tcggttcttc aacaagatca
     3841 aacaatgtcg gcgtgtcgcg acccgatacg ataaactcgc ggccaactat ctggcgttcg
     3901 tcaaactcgc atcaatccga atttggctgc gcgctaatga gtccacgccc tagtgcgggc
     3961 tgcgtggcaa cgataaaggg cggctgttgg cagagcggcc acttgtcgcc gtatggccca
     4021 agcaaggaat ttcggtcggg tcgtttggga cccgcgatcc tgaccaaagc gcgcgatggg
     4081 ctcgaacggc tgataggcca gcgctatcaa tcgtcgaaat cgtcgtcatc gcccctcggc
     4141 gccaccgtgg tcgcacgctg caggaattgc ggcgcctgat agcgcggttc gcgcggagcc
     4201 gggcgctcgg acggatcgcg gtcgattttg gtctttaatt ccacgaggtc ggtgaagaca
     4261 tcggcctggc gccgcagctc gtcggcaatc attggcggct ggctggaaat cgtcgagacc
     4321 acggtgacgc ggacgccgcg gcgctgcacc gcttccacca gcgagcggaa gtcgccgtcg
     4381 cctgagaaca gcaccatctg atcgatatgt tgggcgagtt ccatggcatc gaccgcaagc
     4441 tcgacgtcca tgttaccctt caccttgcgc cggccgctgg cgtcgatgaa ttccttggtc
     4501 gccttggtga cgaccgtgta gccgttataa tcgagccagt cgatcaaggg gcggatcgac
     4561 gagaattcct gatcttcgat gattgccgtg tagtagaacg cgcgcaacag cgttccgcga
     4621 ctttggaatt ccttgagcag gcgcttgtaa tcgatgtcga aaccgagcgt cttggctgtc
     4681 gcgtagagat tggcgccatc gatgaacagc gcaatcctgt tggaggaaga tgacatttaa
     4741 gtaactcacc tttacaaata gatacggggg caagggaccc gctgattcaa actctgcttg
     4801 catatcagga gaggcaaaaa agtcgggcct ctcaaggcat cctcgacaat caaatcgcct
     4861 tagagcgttc gcaaatgcga acgagcgagc gcaaagaggt acaattacaa cgctcccacc
     4921 actctagcat ctaaatgttt taagtttcaa atagttttat ctcgactggt gcgttacttc
     4981 ttgtgagccg gcgcaacacg cttcagcggt tctggcccgc ggccatattc ggggctccga
     5041 ggcgcgctga gcctgatcga tcgatacggc gcggacagca aaagtaggaa gagtgccctg
     5101 tcagccttgt atttggcagc gggtcggctg gcttccgaac cctgcatgac gaccgcccct
     5161 tcggttgtgc agacgtacca ccgccatgtt cggccgcgct tcctcaaggc caattcaaaa
     5221 actggaagct gatccaggca tagcgatcgt gccttgcgaa gcgctttcaa aggggtcccc
     5281 ccatggaaaa atcacttacc cagttggtcg gtggaaatgc acaattggtc ggtcgaaaaa
     5341 cgatcgcttt ttcgtgtagt gaagcctaag aatttccgtc tctcgctgcg gggtcacaaa
     5401 ctttttcagg ccgcgcggag cttgtatcgt cctgtgattg aggcgatagc ccgttagttt
     5461 atcgttagtt ttggatcgat tatcatctga aaaactgttt atttttcaag tatttgtacg
     5521 gaattggcga tttaaggacc tgttaaatat ttccgtcaat tgggcttttg cggcagcacc
     5581 ttgccctcga ttttgggaag ctcgaccgcg gaagcggatc tgtccggcgc ggcgcgccgg
     5641 gccatttcga tcgtcaggcg ctcggccgct tccggcgcca tcaggccgag gatatcggac
     5701 atcttccgtg gcgcgatctg ggaagcgatt tcgaacagca cggacatctc gagccggtcg
     5761 aacaccttgg cggcgtcctt cggcttcatg ccctcataca tggtgatgat gcccttgaag
     5821 cgggcggcat cggtttcggt cttctggtcg gtcgccgtgg tgatccgcga ttcaaccgcc
     5881 ttcaattcct cgacgcgcga atcgatccgc ttctcggcgg ccttgagcag gctctcgcga
     5941 atgtcgatct cgcgggcacg cgcctcaagc tcctgccgcc gcgactgcag ccgctccagg
     6001 atcgcccgct cggaggcgga caccggctgc gactgctcgg gatagaccac gacgccgtcg
     6061 ggcttggcca cttccgggac cggggctggc ggtttggcgg ccgcctcatt cggcttctca
     6121 tgggtcgagc cggtgatgtc agcgggatcg aatttgcttc cgggaaagtt gagggtgtcc
     6181 tgggcccagg atttctggct gctctgcggc tgataatcga agacgtagcc gccatcgaga
     6241 actaacccgg cgaccttcag cacggcaagg ctgaagatcg cgatcaggac caccgggagg
     6301 acgcggatgt cgcggaacga tttcatgcgg cgaggccgtc agaccttctg cggtcggaga
     6361 acgcctgggc cgccgcggca accgccttgg ccgccgatac cttgggtacc gcgggctcgg
     6421 gctcggactc cggctccggc tcggacgccg gccttgccgc ggtcgcgatc ctggacagcc
     6481 gccgaaagat gttgtcgcct tccgcgagct gcttcttcaa ctgcagggac atctgggccg
     6541 cggatgtcag ctggttgccg agattctcgt tgacgtcgcg aacggt
//
LOCUS       DASWGM010000259         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1961834,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000259 DASWGM010000000
VERSION     DASWGM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961834"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..651)
                     /locus_tag="VGA15_07720"
     CDS             complement(61..651)
                     /locus_tag="VGA15_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9587617.1"
                     /translation="MKIYGDTNSGNCLKVKWVCDRLALPYTWIGVDTRKRETRTTPFL
                     KLNSAGQVPAIEFDDGRTLAESNAIIRYLARCSDLIPTDAFAAAKMDEWLFWEQYSHE
                     PYVAVCRFQMVYLGKPASDLDPDKIKRGYAALARMEHQLAATRFLVGDTVSLADVSLL
                     AYTRLAHEGGFHLDGYAAVRRWIGETEKSLGLKPVR"
     gene            complement(733..1602)
                     /locus_tag="VGA15_07725"
     CDS             complement(733..1602)
                     /locus_tag="VGA15_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9587618.1"
                     /translation="MRRLLVAAVTIGVSGAHAADMPDFLRGSIPAAPVTRNWDGWYVG
                     GEASYSAVGADFSKSVVGLTNFIFRDSVLQQPTSQFGLLPKTTTQGTGFGAFVGRNYQ
                     WDDLVFGLEANYSYFDRLSTATSGSNSLQIVNPGGQVAPNVTSTYGVTLTGRSAVQIK
                     DAIAFRARAGWATGDFLPYVFGGIVVGRMDVSRSVTSFVTRRDDTTTTDAFGVTTTTT
                     GPTLFVPAQSQTLSEQRTNAFVAGWTGGLGLEYMLWGNVFMRGEWEYVKFLTVKNTAV
                     QANHLRAAIGYKF"
BASE COUNT          371 a          558 c          556 g          340 t
ORIGIN      
        1 cgcggcgggc gcggcggata agaatcgagg aaatcgcggc catggtcgtt tcgttgaatc
       61 tcaacgcaca ggctttagcc cgagagactt ttcggtctcg ccgatccagc gccgcacggc
      121 ggcatagccg tcgaggtgga aaccgccctc atgcgccagc ctggtatagg ccagcagcga
      181 gacatcggcg agcgagacgg tatcgccgac caggaatcgt gtcgcggcga gttgatgctc
      241 catccgcgcc agcgcggcat agccgcgctt gatcttgtcc gggtcgagat ccgaagccgg
      301 cttgccgaga tagaccatct gaaagcggca caccgcgaca tagggctcgt ggctgtattg
      361 ttcccaaaac agccattcgt ccatcttcgc cgcggcaaag gcgtcggtgg gaatgagatc
      421 gctgcagcgg gcgagatagc ggatgatggc gttggattcc gccagcgtgc ggccgtcgtc
      481 gaactcgatc gccggcacct ggcccgcgct gttgagcttg agaaatggcg tggtgcgggt
      541 ctcgcgcttt cgcgtgtcga cgccgatcca cgtatagggc agcgccagcc tgtcgcagac
      601 ccatttcact ttgaggcaat tgcccgaatt ggtatcgccg tagattttca tcaccgcgcg
      661 gccctcccgg agaggagcgg caatcggacc agccgcatca cttcctgtca aggcggacgc
      721 gcgccgcgag gatcagaact tgtagccgat tgccgcgcgc aggtggttgg cctggacggc
      781 cgtgttcttg accgtcagga atttgacgta ttcccattcg ccccgcatga acacattgcc
      841 ccacagcatg tattccagac cgagaccgcc ggtccagccg gccacgaagg cattggttct
      901 ttgttccgac agagtctgcg attgtgccgg aacgaacaac gtgggtcctg ttgtcgtggt
      961 cgtgacgcca aaggcgtctg tcgtggtcgt atcgtcgcgc ctcgtgacga aactcgtcac
     1021 ggagcgagag acgtccatgc gcccaaccac gataccgccg aatacataag gcaggaaatc
     1081 gccggtcgcc caacccgcgc gcgccctgaa cgcgatcgcg tccttgatct gcaccgcgga
     1141 gcggccagtc aaagttactc cataggtgct ggtgacgttc ggagcgacct gaccgcccgg
     1201 gttcacgatt tggagggagt tggaaccact ggtcgcagta gagagcctgt caaagtagct
     1261 gtagttcgct tcaagaccaa agaccagatc gtcccattga tagttgcggc cgacaaaggc
     1321 gccaaagccc gtgccctgcg tcgtggtttt gggcagcagt ccgaattgcg acgtcggttg
     1381 ctgaagcacg ctgtcgcgaa aaatgaaatt cgtcagaccg acgacgctct tgctgaaatc
     1441 ggctcctacc gccgaatagc tggcctcacc gccgacgtac cagccgtccc agtttcgggt
     1501 gaccggcgcc gcgggaatgc tgccgcgaag gaagtcgggc atgtcggccg cgtgcgcgcc
     1561 ggaaaccccg atcgtcaccg ccgccaccaa tagtctacgc atcgcaacga cccccatcga
     1621 acactctgaa cttggcgccg atcatcgcct gttaacctta atcaatcgtt gtcgcaggcg
     1681 cgtagacgca aaacaaaacg gcgcggaaaa atcccgcgcc gttagcaatc gttaagagat
     1741 gagagctgcg attaaccttt gcgtaccaca ggcggcggcg catacacggt cgggctgtcg
     1801 agattccagc gtacgccgag cgtca
//
LOCUS       DASWGM010000260         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2242167,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000260 DASWGM010000000
VERSION     DASWGM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242167"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VGA15_07730"
     CDS             <1..138
                     /locus_tag="VGA15_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX9587619.1"
                     /translation="VACYMDDLYVYLRARANDATGRVRPAKHEDKPPAAQQAEDSCMG
                     K"
     gene            227..1111
                     /locus_tag="VGA15_07735"
     CDS             227..1111
                     /locus_tag="VGA15_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9587620.1"
                     /translation="MTNAALHRWLLIPLVALMAMLTEREVAWAQTPDSTDLSIELVDP
                     KVLRVCADPRNLPFSNEKGEGFENKLGELFAEKLQKKLDYMYFPQATGFVRMTLAAHR
                     CDVIMGFPQGDDLVQGTNPYYRTAYALVAKQGSGLDDVATLEDERLKGKHIGIVAGTP
                     PATNMAVNGLMANARPYPLMIDTRIDSSAAAMIKDLTSGEIDAAVLWGPMAGYYAKQA
                     NPPLHVTPLVKETTGPRLAYRIGMGVRPADQNWKRQLNRLIQENQPAINKILLDFGVP
                     LLDENDRPITTETATKQP"
     gene            1108..1683
                     /locus_tag="VGA15_07740"
     CDS             1108..1683
                     /locus_tag="VGA15_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HEX9587621.1"
                     /translation="MMWKLAGLITAALALTWPAHAQENAPEPEAYRTDNYRAPVPATL
                     AGARVLSTTEAETIWRAKAAVFIDVLPRPPKPQGLPVGTVWRDKPRLNIPGSIWLPDT
                     GYGTLGAATEDYLRHGLERASGGNLAKLLVIYCQADCWMSWNAAKRMLSYGYPNVAWY
                     PEGTDGWQRANLPVAESQPEPRAGEERSSPQ"
     gene            complement(1710..2324)
                     /locus_tag="VGA15_07745"
     CDS             complement(1710..2324)
                     /locus_tag="VGA15_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587622.1"
                     /translation="MRWRIMICTLLAVAGSAKPCFAADPRYPDWPCAQAKVPEVSIAA
                     VWAGPPLDDVASKWKDDAKVSALLPRLAARRTPLDEAQKTITEFLAGSTAQKTEAGKL
                     LFAGLFDRLNAQRSQVMSGLERVTRKQREAADQIRADTLALQALQDASPPDQPKIDEL
                     GNQLVWEMRIFEDRRRVIKFVCEVPTAIDQRLFALGRVIQQEME"
     gene            complement(2321..3169)
                     /locus_tag="VGA15_07750"
     CDS             complement(2321..3169)
                     /locus_tag="VGA15_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9587623.1"
                     /translation="MSSTIVKDQPTGFSLAQYVTCLNGIVWREGLRFLHQRERFVSAL
                     VRPLVWLFIFAAGFRQVLGISIIPPYETYIPYEVYIAPGLMAMIQLFNGMQSSLSMVY
                     DREMGNMRTLLVSPLPRWYLLFCKLLAGTAVSLLQVYAFLLIAWFWDIEPPPTGYLTV
                     LPALILSGLMLGSLGMLISSGIKQLENFAGVMNFVIFPMFFASSALYPLWRVQEGSPM
                     LYYVCLFNPFTHAVELIRFALYGQMNWVSLAVVGGCTAAFMIGAVFAYDPSRGLSRRG
                     PAGGET"
     gene            complement(3166..>3532)
                     /locus_tag="VGA15_07755"
     CDS             complement(3166..>3532)
                     /locus_tag="VGA15_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9587624.1"
                     /translation="AGSKVRDLSGGQMRRLEIARALLHRPRLLLLDEATVGLDVKARA
                     DILGHVRRLVAEQDIGVLWATHLFDEIIPSDDLVVLHQGRVLARGPVAGIIADTGAHD
                     INASFKRLTETAESGNSSP"
BASE COUNT          721 a         1094 c         1114 g          603 t
ORIGIN      
        1 gtcgcctgct acatggacga cctctacgtc tatctgcgcg cccgcgccaa cgacgcgacc
       61 gggcgtgtgc ggcctgcgaa gcatgaagac aagcctcccg ccgcgcagca ggccgaggat
      121 tcctgcatgg gcaagtgatg atccgaagcc gcaccgtcag gcacttgcgg caggaatttc
      181 gttgatgccc ccgactaatt gcccgatcaa acaggagaag acggcgatga caaatgccgc
      241 gctgcaccgc tggcttttga ttccacttgt tgccctcatg gcgatgctga ccgagcgcga
      301 ggtcgcgtgg gcgcagacgc ctgatagcac tgatctgtcg atcgaactgg tcgatccaaa
      361 ggtgctccga gtatgcgccg acccgcgtaa tctgccgttc tccaacgaga agggcgaagg
      421 attcgaaaac aagctcggcg aactgttcgc cgagaagctt cagaagaagc tcgattacat
      481 gtactttccg caggcaaccg gtttcgttcg gatgacgctt gccgcccacc gttgcgacgt
      541 catcatgggt ttcccgcaag gtgacgatct ggttcagggc accaatccgt attaccgcac
      601 ggcctacgcg ctggttgcca agcagggcag cggccttgat gacgtggcga cccttgaaga
      661 tgagcgtctc aagggcaaac atatcggtat cgttgccggt acgccgcctg ccaccaatat
      721 ggcggtcaat ggtctgatgg caaatgcaag gccgtaccca ctgatgatcg atacccgtat
      781 cgattcgtcg gccgcggcga tgatcaagga tttgacgtcc ggggagatcg acgccgccgt
      841 tctgtggggg ccgatggcag gctactacgc caagcaggcc aacccgccgc tccacgtcac
      901 gcctctcgtc aaggaaacga ccggaccgcg gcttgcctat cgcatcggca tgggtgtccg
      961 gccggccgac cagaactgga aacgacagct caaccgtctg atccaggaaa accagccggc
     1021 catcaacaaa atactgctcg acttcggcgt gccgttactc gacgagaacg acaggccgat
     1081 caccacggag acggcgacga agcagccatg atgtggaagc tcgcgggcct gatcaccgcg
     1141 gcgcttgctt tgacgtggcc ggcccacgca caggaaaacg ctcccgagcc ggaagcgtac
     1201 cgcacggaca attaccgtgc gcctgtcccc gcaacgctcg ccggcgcccg cgttctgtcg
     1261 accacggagg ccgaaaccat ctggcgggcc aaggcggctg ttttcatcga tgtgctgcca
     1321 cgtccgccga aaccgcaagg tcttcctgtg ggcacggtct ggcgcgacaa accgcggctc
     1381 aacatccccg gcagcatctg gctgccggac accggctatg ggacgctcgg ggcagcgacc
     1441 gaagattatc tgcggcacgg gcttgagcgt gcttccggtg gcaaccttgc aaagctgctc
     1501 gtgatttact gccaagccga ttgctggatg tcgtggaacg cggcaaaacg gatgttgtct
     1561 tatggctatc ccaatgtagc ctggtatccc gaaggcaccg acggttggca gcgtgccaac
     1621 ctgccggtgg cggaatcgca gccggagccc agagcaggcg aggagaggtc gtcgccgcag
     1681 tgagagcggc cggcggcatg tcgcgcgcgc tattccatct cctgctggat cacgcggccg
     1741 agcgcgaaca gccgctggtc gatcgcggtc ggtacctcgc agacgaattt gatcacgcgc
     1801 cggcggtcct cgaagatacg catctcccaa accagctgat tgccgagttc atcgatcttg
     1861 ggctgatcgg gcggggaggc atcctgcagg gcttgcagcg ccagcgtgtc ggcgcgaatc
     1921 tggtccgcgg cttcacgctg cttgcgggtc acccgttcga gcccgctcat gacctgggaa
     1981 cgctgcgcgt tgagcctgtc gaaaaggcct gcgaacaata gcttgcccgc ctcggtcttt
     2041 tgcgccgtgg aacccgcaag aaattccgtg atcgtcttct gagcttcgtc gagcggtgtg
     2101 cgccttgcag ccaggcgcgg caataatgcg ctgaccttgg cgtcgtcctt ccatttgctc
     2161 gcgacgtcgt cgagcggcgg acccgcccat accgcggcga tcgagacttc cggcaccttg
     2221 gcctgggcgc acggccagtc cggatagcgc ggatcggcgg caaagcaagg ttttgccgat
     2281 cctgcgaccg cgagcaacgt acagatcatg atgcgccatc tcatgtctcg cctccggcgg
     2341 gtccgcggcg gctgaggccg cgcgatggat cgtaggcgaa caccgcgccg atcatgaagg
     2401 ccgccgtgca gccgccaacc accgccagcg agacccagtt catttgtccg tacagtgcaa
     2461 agcggatcag ctcgaccgca tgggtgaacg gattgaacag gcagacgtaa tagagcatcg
     2521 ggctcccctc ctgcacccgc cacaacggat acagcgcgga cgaagcgaaa aacatcggga
     2581 agatcacgaa gttcatgacg ccggcaaagt tctcaagctg cttgatgcct gaagaaatca
     2641 gcatgccgag cgacccgagc atcaagcctg acaggatcag cgccggcaga accgtgagat
     2701 aaccggtcgg cggcggctcg atatcccaga accacgcgat caacaggaag gcatagacct
     2761 ggagcagcga gaccgcggtg cccgcgagca gcttgcagaa cagaagatac cagcgcggca
     2821 gtgggcttac cagcagggtg cgcatattcc ccatctcgcg gtcatagacc atcgagagcg
     2881 aggactgcat gccgttgaac agctggatca tcgccatcaa tccgggcgcg atatagacct
     2941 cgtaggggat gtaggtttcg tagggcggga tgatggagat gccgagcacc tggcgaaagc
     3001 cggcggcgaa gatgaacagc cacaccagcg gccgaaccag cgccgagacg aagcgttcgc
     3061 gctgatgcag aaagcgcagc ccctcgcgcc agacgatgcc attcaggcag gtgacatatt
     3121 gcgcgagcga aaacccggtc ggttgatcct tgacgatggt ggagctcatg gggagctatt
     3181 cccggactct gcagtttcgg tgaggcgctt gaacgaggcg ttgatgtcgt gagctccagt
     3241 gtcggcgatg atgcccgcga ccgggccgcg cgcgagaacc cggccctggt gcagcaccac
     3301 gaggtcatcg ctggggatga tctcgtcgaa caaatgggtg gcccacaata cgccgatatc
     3361 ttgctcggcg accagccgcc ggacgtgacc aaggatatcg gcgcgggcct tgacgtcgag
     3421 gccgacggtt gcctcgtcga gcagcagcag gcgagggcga tgcagcagtg cgcgggcgat
     3481 ttccagccgt cgcatctggc cgcccgagag gtcgcgcacc ttgctgccgg cg
//
LOCUS       DASWGM010000261         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_854245,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000261 DASWGM010000000
VERSION     DASWGM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854245"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VGA15_07760"
     CDS             <1..958
                     /locus_tag="VGA15_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9587625.1"
                     /translation="RVGVGSVNPTALARGGYWQGRAAEAAGRSQDARAAYTAAAEQST
                     SYYGQLARAKLGLPQIELNGAPAARGRAIERLEIVRAVQLLYGLDEREIAIPIFADMG
                     ENGDPDALVGLGELASRNGDARSMLLLGKAALNRGLPFDFYAYPVMGIPPFKSIGPDV
                     EQSIVFAIARQESAFNPAVVSPAQAYGLMQVTPDAGKYVCKKYGASFDLGRLKTDPVY
                     NAALGAAELGGLIEDYRGSYIMTFAAYNAGRGSVKKWIERYGDPRDPKVDAVDWVELI
                     PFSETRNYVQRIMENLQVYRARFGGGTRLQIEADLHRGASVE"
     gene            complement(1031..>1610)
                     /locus_tag="VGA15_07765"
     CDS             complement(1031..>1610)
                     /locus_tag="VGA15_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020048906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9587626.1"
                     /translation="RWPGKVPAGKVENGIISGLDWFPTFVAAAGNPNIVEELKKGKQI
                     GDTNYKCHLDGYNQMDMITGKGPSNRHEIWYFGESELGAVRIDDYKYRFIDQPAGWLG
                     EKTKPDVPYLINLRLDPFERTGWPDSGTKYGAQQYFDWFKYEFWRFVFVQQQVEKLAM
                     TAIEFPPMQRGASFNLDAVKAKIAAAQASIAK"
BASE COUNT          297 a          502 c          508 g          303 t
ORIGIN      
        1 gcgcgtcggc gtcggcagcg tcaatccgac cgcgctcgcg cgcggcggct attggcaggg
       61 ccgtgccgcc gaggcggcgg gccgctcgca ggacgcccgc gccgcctaca cggcggcggc
      121 agaacaatcg accagttatt acggccagtt ggcgcgtgcc aaactcggat tgccgcagat
      181 cgaactgaac ggcgcgccgg cggcacgcgg acgtgcgatc gaacggctgg agatcgtccg
      241 cgcggtgcag ctgctctacg gcctcgatga acgtgaaatc gcgatcccaa ttttcgccga
      301 catgggcgaa aacggcgatc ccgacgcgct ggtggggctc ggcgaactgg cctcccgcaa
      361 tggcgacgcg cgcagcatgc tgctgctcgg caaggccgcg ctcaatcgcg ggctgccatt
      421 cgatttctac gcctatccag tgatggggat accgccgttc aagtcgatcg ggccggacgt
      481 cgagcagagc atcgtgttcg ccatcgcacg gcaggagagc gccttcaatc cggcggtggt
      541 gtcaccggcg caagcctacg ggctgatgca ggtgacgccg gatgccggaa aatacgtatg
      601 caagaaatat ggcgccagct ttgacctcgg ccggctgaag accgatccgg tctataacgc
      661 ggcccttggc gccgcggagc tcggcggctt gatcgaggat tatcgcggct cctacatcat
      721 gactttcgcg gcctacaatg ccggccgcgg cagcgtcaaa aaatggatcg agcgttacgg
      781 tgatccgcgc gatcccaagg tcgatgccgt cgattgggtg gaattgatcc cgttctccga
      841 gacccgcaat tacgtgcagc gaatcatgga gaatctgcag gtctatcgcg cgcgattcgg
      901 cggcgggacg cggctgcaga tcgaggccga tctgcaccgc ggcgccagcg tcgaataggc
      961 aagcgcttgt caagcgggca ccccgggccc agggtgcccg ccacgacgat cagtcaaagc
     1021 cctggcggac ctactttgcg attgaagcct gggctgccgc gatcttcgcc ttcacggcat
     1081 cgaggttgaa actcgcgccc ctttgcatcg gcggaaactc gatcgccgtc atggcgagct
     1141 tctccacctg ctgctgaaca aacacgaagc gccagaattc atatttgaac cagtcgaagt
     1201 attgttgtgc gccgtatttg gtcccgctgt cgggccagcc cgtgcgctcg aaaggatcga
     1261 ggcgaaggtt gatcaaatag ggcacgtcag gcttggtttt ttcacccagc cagcctgcag
     1321 gctggtcgat gaaacgatac ttgtagtcgt cgatgcgcac ggcacccagc tcgctttcgc
     1381 cgaagtacca gatttcgtgc cggttcgacg gccctttgcc ggtgatcatg tccatctggt
     1441 tatagccgtc cagatgacat ttgtagttgg tgtcgccgat ctgcttgccc ttcttcagct
     1501 cctcgacaat gttcgggtta ccagccgccg caacgaaggt cggaaaccag tccagcccgg
     1561 agatgatgcc gttctcgacc ttgccggccg gcaccttgcc cggccagcgg
//
LOCUS       DASWGM010000262         1741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2242285,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000262 DASWGM010000000
VERSION     DASWGM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242285"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1551
                     /locus_tag="VGA15_07770"
                     /pseudo
     CDS             1..1551
                     /locus_tag="VGA15_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198369.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
BASE COUNT          336 a          548 c          554 g          303 t
ORIGIN      
        1 ggcgtggtgc tggtgacgtc ggggccgggc gccaccaaca tggtgacgcc gctgaccgac
       61 gcgctgatgg attcgatccc gctggtgtgc atcaccgggc aggtgccgac ccacctgatc
      121 ggcaatgacg cgttccagga atgcgacacc gtcggcatca cccggccctg caccaagcac
      181 aactggctgg tgcgtaacgt caatgatctg gccaaggtgc tgcacgaagc gttctacgtc
      241 gccaacacct gacggcccgg ccctgtcctg gtcgatgtgc cgaaggacgt gcagttcgcg
      301 gtcggcacct atcacccgcc gcgcaagtcc gatgtgcatg tgtcctactc gccgcgggtg
      361 aagggcgacg cggcgcagaa ccgcaaagcc gtggcgctat tggcctcggc caatcggccg
      421 gtgatctata gcggcggcgg cgtcatcaat tccggccgcg aggcctcaaa gctgctgcgc
      481 gaactggtcg aagccacggg gtttccgatc acctccacct tgatgggtct cggcgcctat
      541 ccggcgtcgg gcaagaactg gctcggcatg ctcggaatgc acggcaccta tgaagccaac
      601 atggcgatgc acggctgcga cgcgatgctg tgcgtcggcg cacgcttcga cgaccgcatc
      661 accggacgcg tcgacgcgtt ctcgccgggc tcgaagaaga tccacatcga tatcgatccc
      721 tcctcgatca acaagaacat ccgcgtcgat attccgatca tcggcgatgc cggcaatgtg
      781 ctcggcgatc tgttgcaggt gttcaaggcg gaggcgaaaa agccggacat ccggccgtgg
      841 tggcaggaga tcgcgaaatg gcgcgcgcgc aattcgctgt cgtacaaaaa gaacagcgat
      901 gtcatcctgc cgcaatacgc cattcagcgg ctgttcgaga atacacgcgg ccgcgacgtc
      961 tacatcacca ccgaagtcgg ccagcaccag atgtgggcgg cgcaattctt cggcttcgag
     1021 gagccgcacc gctggatgac ctcgggcggt ctcggcacca tgggctatgg tttgccggcg
     1081 gcggtcggcg tccaggtcgc gcatcccgac agtctggtga tcgacatcgc cggcgacgcc
     1141 tcggtgcaga tgacgatgca ggagatgtcg acggccgttc aatatgaatt gccgatcaag
     1201 atcttcatcc tgaacaacca gtatatgggc atggtgcggc agtggcagca gctgctgcac
     1261 ggcaaccggc tgtcgcacac ttactccgaa gcgctgccgg acttcgtcaa gctcgcggac
     1321 gccttcggct gcgtcggcat ccaggcgatc aagcccggcg atctcgacgg cgccatcaag
     1381 gagatgatca acgtcaagcg gccggtgctg ttcgattgcc gggtggcggc gctggaaaat
     1441 tgtttcccga tgatcccgtc cggcaaggcg cacaacgaaa tgctgttgcc ggcggaagcc
     1501 aatgacgaag ccaccgccgc ggccttcgcc ggcggcaagg cattggtgtg agggtgagac
     1561 aagccaatgt ttgacctgca gcaactcgaa cgtgcccaaa acatcgtcgc cgcggcgatg
     1621 cctccgaccc cggcgcatca atggccgctg ctgtccgagc ggctcggtgc gacggctatt
     1681 gtcaagcatg agaaccacac gccgaccggc gccttcaagg tgcgtggcgg gctcgtctat
     1741 g
//
LOCUS       DASWGM010000263         4853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_295674,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000263 DASWGM010000000
VERSION     DASWGM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295674"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VGA15_07775"
     CDS             complement(<1..138)
                     /locus_tag="VGA15_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX9587627.1"
                     /translation="MTTGTALLDFAKRLDQTAEDTEALLSKLLSGELLPDEIARPKRL
                     IE"
     gene            264..545
                     /locus_tag="VGA15_07780"
     CDS             264..545
                     /locus_tag="VGA15_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX9587628.1"
                     /translation="MPAYVIFDVEIRDPARYQDFMPVLQAVGARYLARGGAHEVYEGD
                     WSPRRIVKLEFQSKGVGEAFCHGPVYQGLKHIRDECSSARLVSVEGLEA"
     gene            569..1249
                     /gene="mtgA"
                     /locus_tag="VGA15_07785"
     CDS             569..1249
                     /gene="mtgA"
                     /locus_tag="VGA15_07785"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX9587629.1"
                     /translation="MRQAIRAVVLIVLVVLLLPYLLAPLYRVGHPVSTLMVRRWLTGA
                     PVSRQWIDFAAISPLLPRSVVASEDAKFCSHHGIDWDALRDVVDDAEDSEVTRGGSTI
                     TQQVAKNLFLWPGRSVVRKALEFPLALWIDLVLPKQRILEIYLNIAELGPSGQFGAQA
                     GALYAFDHPALTLSPREAALLAAILPNPVKRSARNPGAGVRRLAGTYTARAQSAGLQR
                     CWSENRAF"
     gene            1397..1582
                     /gene="rpmF"
                     /locus_tag="VGA15_07790"
     CDS             1397..1582
                     /gene="rpmF"
                     /locus_tag="VGA15_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX9587630.1"
                     /translation="MAVPRRKTSPSRRGMRRSADALKKPTYAEDKDSGELRRPHHLDL
                     KTGMYKGRQVLKAKKES"
     gene            1979..2800
                     /locus_tag="VGA15_07795"
     CDS             1979..2800
                     /locus_tag="VGA15_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587631.1"
                     /translation="MSMIGFPLLLISLAIYNIIVFLMPGVSFADPVVKLTLMSGAEWP
                     LTLGDMLLALAILLLLSEVIKGARPGAKYLTDHLLSLIVFGGAAAEFVLWPKFGTSTY
                     FLLTMLALVDFLSGVALRARRGTLVTSGPAVVSTKGARASSPAATETRFVPEPAPSVP
                     AAAAVAESVLLDHPQPKIPRPEIQRPEVRPNPPPPEIVQAPSPEVVNAPSPEVVNAPS
                     PEVVSAPSPEVVSAPSPEPVNAPSPEVANAPSPEVTSPDLQPESSSPPSPEKPEH"
     gene            complement(2852..3115)
                     /locus_tag="VGA15_07800"
     CDS             complement(2852..3115)
                     /locus_tag="VGA15_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587632.1"
                     /translation="MDDGALPDAAPASVALVPMVASAQRSQPLIRPLSRPSSIFVTHL
                     IATAEQAPQTRSLRRATPADAQTAYQANQRTVADASLRTRQII"
     gene            3363..>4853
                     /locus_tag="VGA15_07805"
     CDS             3363..>4853
                     /locus_tag="VGA15_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9587633.1"
                     /translation="MTSTVPQASTILASLGQAAFVWDIATDAIAWSDHAAAVFPDIPA
                     AALASGAEFAKLIEPVRSIRTDALGHASPARGGTPYRIEYGVRTSTSAPVLWIEESGC
                     WFAGADGRPARAQGIVRVNDERHARDAQLVRLLRHDPLTGELNRTHLVASLAESIEEA
                     ARFRASSAFMLIGIDRLARINDAFGFDVADAVISEVATRIRARLRNGDVLGRFSGNKF
                     GLILKNCTVDDINIAAERFLAGIRDDVVPTKSGPVSVTASIGAVSVPRYARSADEAVN
                     RAQETLDMAKRRRAGSFSLWRPNVERDAQRRVNIRVTDEIVTALNERRIAMAFEPVVE
                     ARSRDTAFYECLVRMEQDDGQVLLAPDIVPIAEKLGLIRLVDHRVLELVVAELAGSTN
                     VQLSLNISPDTTMDPDWWASIESLMRAHPGVAERLIVEITETVAIQDIDGLRGFVTRL
                     KNFGSRIAIDDFGAGYTSFRNLRKLGVDIVKIDGAFVQNIARSADDR"
BASE COUNT          826 a         1542 c         1547 g          938 t
ORIGIN      
        1 ttcgatcagg cgtttcggcc gcgcgatctc gtcgggcaaa agttcaccgg ataacaactt
       61 cgagagcagc gcttcggtgt cctcggcggt ctggtccagt cgtttcgcaa agtctaatag
      121 tgcagtgccg gtcgtcatta agtatagctc cagatcgagt tcggtcggga caatcggtta
      181 tggcatcggc ttcgtcaatt ccgccgtgac ggccctcacc cggcgtctgg ccgtcatatg
      241 atccggtcca aaaggagttc ctggtgcctg cttatgtcat ttttgacgtc gaaatccgcg
      301 atcccgcgcg ttaccaggat tttatgccgg tgctgcaggc ggtcggggcc agataccttg
      361 cgcgcggtgg tgcgcacgag gtctatgaag gcgattggtc gccgcgccgc atcgtcaaac
      421 ttgaatttca atccaaggga gtgggggaag ccttttgcca tggaccggtg tatcagggcc
      481 tcaagcatat tcgcgatgaa tgcagttcgg cgcggctcgt gagcgtggaa ggtcttgagg
      541 cctgatgcgg cagacgatcc gcgccctgat gcggcaggcg atccgcgccg tggtgctgat
      601 cgtgctggtc gtgctgttgc tgccatacct gctggcaccg ctgtaccgcg tcggccatcc
      661 ggtctccacc ctgatggtcc ggcgttggct gaccggcgcg ccggtgtcgc ggcaatggat
      721 cgatttcgcc gcgatctcgc ccttgctgcc gcgctcggtg gtcgcgtccg aggacgcgaa
      781 attctgcagc catcacggca tcgactggga cgcgctgcgc gatgtggtgg acgacgccga
      841 ggatagcgag gtgacccgcg gcggttcgac catcacccag caggtcgcga aaaacctgtt
      901 cctgtggcca ggccgcagcg tggtccgcaa ggccctggag tttccgctcg cgctgtggat
      961 cgatctggtg ctgccgaagc agcggatcct ggaaatctac ctcaacattg ccgaactcgg
     1021 gccctcaggc cagttcggcg cgcaggccgg cgccctctat gccttcgacc atccggcctt
     1081 gacgctgtcg ccgcgcgagg cggcgctgct ggcggcgatc cttcccaatc cggtcaagcg
     1141 cagcgcccgc aatcccggcg ccggggtgcg gcgtctggcc gggacctaca cggcccgtgc
     1201 gcagtccgcc ggattgcagc ggtgctggag tgaaaatcgc gctttttgag ccagttgggt
     1261 gtattttgaa cctagccttg gactacccca tcctctataa gcgcggcctt atcggcattt
     1321 cagctcgtgg cctaaaaacg cttcagccgt ccactttggg acgatgcctc cgacaacacc
     1381 cctagaggac accgatatgg ccgttccccg cagaaaaaca tcgccctcgc ggcgtggcat
     1441 gcgccgctca gcggacgcat tgaagaagcc gacctatgcc gaggacaagg attccggcga
     1501 attgcgccgc ccgcatcatc tcgacttgaa gaccggcatg tacaagggcc gacaggttct
     1561 gaaggccaag aaagagtcct gatggggccg gtcgcggctt gagcgggcgc tgggcccgtc
     1621 gctcgacatc cggcccgcct gcagcagcgc accgcgatac cgtcgaagac gcgcgtgaac
     1681 gcgctgatgc cgcgtcgaag aggcgcggga acgcgctgat ggcgttgcgc agcgtccggg
     1741 gcgcctcgac ttgcgaaggc caacgcggac aagtgttccg gtgcggcacg ccgggtaccc
     1801 gccgcgggta gcccgcgcac agcctccgtg acgcaacaca gagccgaaat ccctgtgatc
     1861 ggcagcatcg gcctgaattc ggccaatgag tgaggtaaga cggtttcctt taggccaacc
     1921 caacagcgat tcgcttaagg aatgccttgg ttctcctgtt cgaaagaagg cctcgccgat
     1981 gtcgatgatc ggttttccgc tgcttctgat ttcgctcgcg atctacaaca tcatcgtctt
     2041 cctgatgccc ggcgtctcct tcgccgaccc ggtcgtgaag ctgaccttga tgtccggcgc
     2101 cgagtggccg ctgacgctgg gcgacatgct gctggcgctc gccattttgt tgttgctgtc
     2161 tgaggtcatc aagggcgcgc gtccgggagc gaaatatctc accgaccatt tattgtcgct
     2221 gatcgtgttc ggcggcgcgg ctgccgaatt cgtgttgtgg ccgaagttcg gcacctcgac
     2281 ctacttcctg ctgaccatgc tggcgctggt ggattttctg tcgggcgttg cattgcgtgc
     2341 gcggcgcgga acgctggtga catcgggccc tgctgtggtc tccaccaagg gcgcgcgagc
     2401 atccagcccc gccgcgaccg aaacgcgatt tgttcccgaa cctgcgccgt cggtgccggc
     2461 agcggccgcg gtcgccgaat ccgtactgct ggatcacccg caaccgaaaa tcccgcgccc
     2521 cgaaatccaa agacccgagg tcaggccaaa tcccccgccg ccggagattg tacaagcgcc
     2581 atcgccggag gtggtaaatg caccttcgcc tgaggttgtt aacgcgccat ctccggaggt
     2641 ggtaagtgca ccatcgcccg aggttgttag cgcgccatcg ccggaaccgg taaatgcacc
     2701 atcgccggag gtggcgaatg ctccgtcgcc ggaggttacc tcgccagacc tgcagccgga
     2761 gagcagttcg ccaccgtctc cggaaaaacc ggagcattga tttttcttca gccccttagg
     2821 ggagggtgaa agggcgggct gacggcccga tctaaatgat ttgccgcgtc cgaaggctag
     2881 cgtccgcgac ggtgcgttgg ttcgcctggt aagcggtctg cgcgtcggcg ggcgttgccc
     2941 ggcgcaggct gcgggtttgc ggtgcctgct cggcggttgc gatcagatgc gtgacaaaaa
     3001 tcgaactcgg cctcgacagc ggccggatca aaggctgcga ccgttgcgcc gaggcgacca
     3061 tcggcaccag cgcaacgctg gcgggcgctg cgtccggaag ggcgccatca tccaaggtct
     3121 gctgtggtcg atcacggctt aacatcgccc gtcgaaatcc gctgaactgt cacgttttgg
     3181 gacgttgcgc ccccttagcg acgtcttcat cgcaagggtc atgccggttg tccgcggctc
     3241 gttcccagcc gccgaacagc atggttggcg gaatatttat gaactttgcc tagggttggg
     3301 gccgaggcag gcagtattct aggaccgaga atcacccagc cgtgtcccga aacgaggcga
     3361 ttttgacatc caccgttccg caagcttcga ccatcctggc ctcgctcggc caggcggcct
     3421 tcgtgtggga catcgcaacc gatgcgattg cctggagcga tcacgctgcc gcggtttttc
     3481 cggatattcc ggcggcggcg ctggcgagcg gcgcggagtt tgcaaaactg atcgagccgg
     3541 tgcgctcgat ccggaccgac gcgcttggcc atgcatcgcc cgcgcgcggt ggcacgccct
     3601 atcggatcga atatggcgtc cggaccagca catccgcccc cgtgctctgg atcgaggagt
     3661 ccggctgctg gttcgccggc gccgacggcc ggccggcgcg cgcgcaaggc atcgtccgtg
     3721 tcaatgatga gcggcatgcc cgcgacgcgc aactggtgcg gttgttgcgc cacgacccgc
     3781 tcaccggcga gctcaatcgt acgcatctgg ttgcatcgct tgccgagtcc atcgaggaag
     3841 ccgcgcgctt ccgcgcctct tccgccttca tgctgatcgg catcgatcgt ctggcgcgca
     3901 tcaacgacgc cttcggcttc gacgtcgcgg acgcggtcat ttccgaggtc gcaacccgca
     3961 ttcgcgcgcg gctgcgcaat ggcgatgtgc tcggacgatt ctccggcaac aaattcggac
     4021 tgatcttgaa gaactgcacc gtcgacgaca tcaacatcgc cgccgagcgg tttctggccg
     4081 gcatccgcga cgatgtggtg ccgaccaaat ccggccccgt gtccgtgacg gcatcgatcg
     4141 gcgcggtcag cgtgccgcgt tacgcgcgca gcgccgatga ggccgtcaac cgcgcccagg
     4201 aaacgctcga tatggcgaag cgacgccgcg cgggatcgtt ttcgctctgg cgcccgaatg
     4261 tcgagcgcga cgcccagcgc cgcgtcaata tccgcgtcac cgacgaaatc gtcaccgcgc
     4321 tcaacgagcg gcggatcgcg atggcgttcg aaccggtggt ggaggcccgc tcgcgcgaca
     4381 ccgcgttcta cgagtgtctc gtccgcatgg agcaggatga cgggcaggtt ctgctggcgc
     4441 cggacatcgt cccgatcgcc gagaagctcg gcctgatacg tctggtcgat caccgcgtgc
     4501 ttgaactcgt ggtggccgaa ctcgcaggct cgacgaacgt gcagctcagc ctcaacatct
     4561 cgcccgatac cacgatggac ccggactggt gggcttcaat cgaatcgctg atgcgcgcgc
     4621 atcccggcgt ggccgagcgg ctgatcgttg aaatcaccga aacggttgcg atccaggata
     4681 tcgacggcct gcgcggcttt gtcacgcggc tgaaaaactt cggcagccgg atcgcgatcg
     4741 acgatttcgg cgccggttac acctcgttcc ggaatttgcg caagctcggc gtcgatatcg
     4801 tgaagatcga tggggccttc gtgcagaaca tcgcccgctc ggccgacgat cgc
//
LOCUS       DASWGM010000264         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2381292,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000264 DASWGM010000000
VERSION     DASWGM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381292"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VGA15_07810"
     CDS             <1..173
                     /locus_tag="VGA15_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX9587634.1"
                     /translation="ALAKLENLAKERGTAVGVAAALPVSIERIGVWIKALEGHGIMLV
                     PLTTAMLKSKSG"
     gene            247..750
                     /locus_tag="VGA15_07815"
     CDS             247..750
                     /locus_tag="VGA15_07815"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX9587635.1"
                     /translation="MARYEDLPYRTCVGMMLINAAGLVFIGRRAGGIEHVDQSHVWQM
                     PQGGVDPGEDTWAAAKRELYEETSVRSVEKLGEVADWLIYDIPRTVAGRTWKGRYRGQ
                     RQKWFAVRFTGQDAEINVAAPGGGHKAEFVSWRWEPMKNLPDLIVPFKRPVYERVVKE
                     FSKLAGS"
     gene            757..1260
                     /locus_tag="VGA15_07820"
     CDS             757..1260
                     /locus_tag="VGA15_07820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX9587636.1"
                     /translation="MPETKPYRPNVGIALFNAAGQVLIGRRFKNDGPEIILPGLEWQM
                     PQGGIDENESPRDAVMRELWEETGVTNAGYLGETDWLTYEFPPFAVPPSHRLAKFRGQ
                     RQKWFALRFTGSDDEIDPLTPRNGQPAEFDQWRWERLDRVPDLVVPFRREVYRTVAQA
                     FAKFAGV"
     gene            complement(1283..2902)
                     /locus_tag="VGA15_07825"
     CDS             complement(1283..2902)
                     /locus_tag="VGA15_07825"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9587637.1"
                     /translation="MKRKIAAIFAADIAGYSRLVAEDEEETLRRLASYRQVTDDFIAR
                     SGGRIFNTAGDAVLAEFPSAVEAVRCAIDIQESLRTRNMAYPASRQMSFRIGITIGDV
                     VERDGDLLGDGVNIAARLEGLAEVGGICISRAVHEQVANKLSVQFADIGAQEVKNIPT
                     PVHAYMVAMRREDGSYSMPQVKKKSSGAAPTPNWMWPAAVTVICLVAIGVGGFLYFTK
                     MEMPAGSKGSASASNDAAPSSPVASTPAPKIVAPAPQLSASPPPGRSSEKFAAETVPF
                     VSDRTRLALSNEYVPATDHKAFALNVTGTYGFVTAQPNEEAAKSAALDQCQKRADAIQ
                     SPRRCELYAVGNTTVYPHGKPPLPPLPWIRHDPSIEKPFVAKDMPLIRDLGKSRLEST
                     YAPGKKSKSIAVGPGGQFIDNIGVETVEDSVRRNLEACGAIAGVPCMIVAVDDTFVVP
                     VPTTLKVTGFFRAGGNSSIAADARDDVSRKFAGAASGWNAVAVGASLRPGLALKAANE
                     QNAVNEALGNCVKHDSDCHVIAIGPFSVGPN"
     gene            complement(3085..3492)
                     /locus_tag="VGA15_07830"
     CDS             complement(3085..3492)
                     /locus_tag="VGA15_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX9587638.1"
                     /translation="MATFHFDLVSPEKLAFSGEVDQVDVPGIEGDFGVLAGHAPVVAA
                     IRPGILTVTSGGNHQKIIVLGGLAEVSDKGLTVLADVATSLQDLDRARFADKISEMEA
                     KLAEKDGSELDRAIERLDHYKSIQHQLNATAMH"
     gene            complement(3606..>3932)
                     /locus_tag="VGA15_07835"
     CDS             complement(3606..>3932)
                     /locus_tag="VGA15_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9587639.1"
                     /translation="ARMVQQILQKYKSLQDIIAILGMDELNEEDKVTVARARKIERFL
                     SQPFFVAEVFTGSPGKFVDLADTIKGFRGLCEGKYDHLPEQAFYMVGTIEEAVEKGKK
                     LAAEAA"
BASE COUNT          767 a         1192 c         1213 g          760 t
ORIGIN      
        1 gggccctggc aaagcttgaa aatctggcga aggaacgcgg aaccgcggtc ggcgtcgccg
       61 cggcgctgcc ggtctcgatc gagcggatcg gcgtctggat caaggcgctt gagggccatg
      121 gcatcatgct tgtgccattg acaaccgcga tgctgaaatc aaaatccggc tgaggcaagt
      181 ttcgggctgg cgcgcctttc gaaccggccg ggtctcgccc atatgaccgt aaaaggcttt
      241 cacggaatgg cgcgctacga agacctgccc tatcgaacat gcgtcggcat gatgctgatc
      301 aacgcagccg ggctggtctt cattggtcgc cgcgccggcg gcatcgaaca tgtcgaccaa
      361 tcccatgtct ggcagatgcc gcagggcgga gtcgatcccg gcgaggatac ctgggcggcg
      421 gcgaagcgtg aactttatga ggagaccagt gtccgatcgg tcgagaagct cggcgaggtc
      481 gcggactggc tgatctatga cattccccgc accgtcgccg gacggacctg gaagggccgc
      541 tatcgtggcc agcggcaaaa atggttcgcg gttcgtttca ccggccagga cgccgagatc
      601 aatgtcgccg ccccgggcgg cggccacaag gctgaattcg tcagttggcg ctgggagccg
      661 atgaagaatc ttcccgacct gatcgttccg ttcaaacgcc cggtctatga gcgcgtggtg
      721 aaggaatttt cgaagctggc aggcagctag cgcaggatgc cggagaccaa accctatcgt
      781 cccaatgtgg ggatcgcgct gttcaacgct gccggccagg tgctgatcgg gcggcgcttc
      841 aagaacgacg gaccggaaat cattcttccc ggccttgaat ggcagatgcc gcaaggcggt
      901 atcgacgaaa acgaaagtcc gcgcgacgcg gtgatgcgcg aattatggga agagaccggc
      961 gtaaccaacg ccggctatct cggcgaaacc gactggctga catacgagtt tccgccattc
     1021 gccgtgccgc cgtcacatcg gctcgccaaa ttccgcggcc agcgccagaa atggtttgca
     1081 ctgcgtttca cagggtccga tgacgaaatc gatccgctga caccacgcaa cggtcagccg
     1141 gcggaattcg accaatggcg atgggaacgg ctcgaccgcg tccccgacct cgtggtgccg
     1201 ttccgacgcg aggtgtatcg gacggtggcg caggcttttg cgaaattcgc gggggtatga
     1261 agcttgagca gaggtcagaa gcttaattcg gcccaaccga aaacggaccg atcgcgatga
     1321 cgtggcaatc gctgtcgtgc tttacgcagt tgcccaaggc ttcgttgacc gcgttttgtt
     1381 cgttcgccgc cttgagcgcc agcccgggac gcagggatgc gccgacggcg acggcgttcc
     1441 acccggatgc cgcacccgcg aattttcgag acacatcatc cctggcatcg gctgcaattg
     1501 aagaattgcc gccggcccga aagaagccgg tcaccttcaa tgtggtgggc accggcacga
     1561 caaatgtgtc atcgacggcc acgatcatgc aaggaacgcc tgcaatggcg ccgcaggctt
     1621 ccaggttccg acgcaccgaa tcctcgaccg tttcaacgcc gatattatcg atgaattgcc
     1681 cacctggacc gaccgcgatc gacttcgact tcttgcccgg cgcataagtg ctctcaagcc
     1741 tggactttcc gaggtcgcga atcagcggca tgtccttggc tacgaagggc ttctcgattg
     1801 aaggatcatg cctgatccat ggcagcggcg gcaacggcgg cttgccatgc ggatagactg
     1861 tcgtgttgcc cacggcatac agctcgcatc ttcgcggcga ctgaatggcg tcggctcttt
     1921 tctggcattg gtcgagcgcc gcgctcttcg cagcctcttc gtttggctga gcggtaacga
     1981 atccgtaggt gcccgtcaca ttgagggcga aagccttgtg atctgttgcc ggcacatact
     2041 cgttcgaaag cgcaagacgg gtacgatcgc tgacgaacgg cacggtctcg gccgcgaact
     2101 tttcgctcga acgaccaggc ggcggcgaag cggatagctg aggcgccggt gcgactatct
     2161 ttggagcggg cgtcgaagcg accggcgatg aaggcgccgc gtcattgctc gccgaagctg
     2221 aacccttcga cccggccggc atttccatct tggtaaaata caggaagccg ccgaccccaa
     2281 tcgccacgag acagatcacc gtcacggctg ccggccacat ccagtttggt gtcggcgccg
     2341 cgcctgaaga cttcttcttc acttgcggca ttgaatagct gccgtcctcg cgccgcatcg
     2401 ccaccatgta ggcgtgcacg ggagtgggaa tattcttcac ttcctgcgcg ccgatatcgg
     2461 cgaactgcac cgacagcttg ttggcgacct gctcatgcac cgcacgtgaa atgcagatgc
     2521 cgccaacctc ggcgagacct tccaggcggg ctgcgatatt gacgccatcg ccgagcaggt
     2581 cgccgtcgcg ctcgaccacg tcgccgatcg taatgccgat gcgaaagctc atctgacggc
     2641 tggcgggata cgccatgttg cgggtgcgca ggctctcctg gatgtcgatc gcgcaacgca
     2701 ctgcttcgac ggcgcttgga aattcggcga gaacggcgtc gccggcggtg ttgaaaatac
     2761 ggccgccgga tctggcgatg aagtcgtcgg tcacctgacg ataggatgcg aggcgccgca
     2821 gcgtttcctc ttcatcttcg gcaaccagcc tgctgtagcc agcaatatcg gccgcaaaaa
     2881 tcgccgcgat cttgcgcttc ataaagtcca gccccggaca accatctatt gcggcagaat
     2941 gccgccggaa tgcgcagggt gcaaccgggg atgtgtagcc catccttcgc gacgcaggcc
     3001 taagaaggcg cgcgtcctcc agcgatgagc gcaattgcgc tcacgcgggg atgacgggga
     3061 taattcggga aaccgcttca atctctagtg catcgccgtc gcgttgagct ggtgctggat
     3121 gctcttgtaa tgatcgagcc gctcgatcgc gcggtcgagt tcggagccgt ccttctcggc
     3181 gagcttggct tccatctcgg aaatcttgtc ggcgaaccgg gcccggtcga ggtcttgcag
     3241 cgaagtggcg acgtccgcca gcaccgtcag acccttgtcg gacacttcag caaggccgcc
     3301 gagtacgatg atcttctggt ggttgccgcc cgacgtcacc gtcaggattc ccgggcggat
     3361 cgcggccacc accggcgcat gtccggcgag cacgccgaaa tcgccctcga tgccgggcac
     3421 atcgacctga tcgacttcgc ccgagaaggc gagtttttcc ggtgagacga gatcgaagtg
     3481 gaaggtggcc atgggagcta ctctctcagt ggtcacccgc gggcttgacc cgcgggtcca
     3541 tctatcaaaa aaatctttcc agcgatggat tgccgggtca agcccggcaa tgacgacagg
     3601 ttatattacg ccgcctcggc agccagcttc ttgccctttt cgacggcctc ttcgatggtg
     3661 ccgaccatgt agaaggcctg ctcgggcagg tggtcgtact tgccttcgca aagtccgcgg
     3721 aaacccttga tggtgtcggc gaggtcgacg aacttgcccg gcgatcccgt aaaaacttca
     3781 gcaacgaaga acggctgcga caggaagcgc tcgatcttgc gcgcacgtgc gacagtgacc
     3841 ttgtcctctt cgttcaattc gtccatgccg aggatggcga tgatgtcctg cagcgacttg
     3901 tacttctgca gtatctgctg aaccatgcgc gc
//
LOCUS       DASWGM010000265         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2652383,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000265 DASWGM010000000
VERSION     DASWGM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2652383"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VGA15_07840"
     CDS             <1..727
                     /locus_tag="VGA15_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX9587640.1"
                     /translation="NSLSFAENFLNMCFAVPCEEYKINPVLADALDKIFILHADHEQN
                     ASTSTVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALAMLAEIGTVDKIPDFIKR
                     AKDKNSDVRLMGFGHRVYKNYDPRAKIMQRMCHAVLAETGHDDDPMLKVALELEKIAL
                     SDEYFIDRKLYPNVDFYSGITLKAMGFPTSMFTVLFAVARTVGWISQWSEMIEDPHQK
                     IGRPRQLYTGVTHRDYVDIDKRK"
     gene            complement(801..1082)
                     /locus_tag="VGA15_07845"
     CDS             complement(801..1082)
                     /locus_tag="VGA15_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9587641.1"
                     /translation="MTIPFEKLKARLLANPKVKVEYEALAPEFEISVELVKARLRARL
                     SQAELAARMGTSQSTIARLESGQTLPTTKTLLRYAEATGSRFHVRLSAA"
     gene            complement(1079..1255)
                     /locus_tag="VGA15_07850"
     CDS             complement(1079..1255)
                     /locus_tag="VGA15_07850"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9587642.1"
                     /translation="MEGKLWELRLTGRDGIARALYVTAIGRRVVVVRAFVKKTQRTPP
                     AEIQLALRRAKEIT"
     gene            complement(1456..>1842)
                     /locus_tag="VGA15_07855"
     CDS             complement(1456..>1842)
                     /locus_tag="VGA15_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX9587643.1"
                     /translation="AFRIAHAAFVKSGTATLELAIAGVPMVAAYKVGAVEAAIMRRAI
                     RTSSVILANLVIGENVVPEFIQEDCAPEKLLPALRDILTDSPLRQRQVAAFAKLDAIM
                     STGNQPPSVRAADIVLATLRKARGSN"
BASE COUNT          371 a          588 c          532 g          351 t
ORIGIN      
        1 gaactcgctg agcttcgccg agaattttct gaatatgtgc ttcgcggtgc cctgcgagga
       61 atacaagatc aatccggttc tcgccgacgc gctcgacaag atcttcatcc tgcacgccga
      121 ccacgagcag aacgcctcga cttcgacggt ccggatcgca ggctcctccg gggccaaccc
      181 gttcgcctgc atcgccgccg gcatcgcctg cctgtggggc ccggcccatg gcggcgccaa
      241 cgaagcagcc ctcgcgatgc tcgccgaaat cggcacggtc gacaaaattc ctgacttcat
      301 caaaagggcg aaagacaaga acagcgacgt tcgcctgatg ggttttggcc atcgcgtcta
      361 caagaactac gatccgcgcg ccaagatcat gcagaggatg tgtcacgccg tgctggcgga
      421 gaccggccac gacgacgatc cgatgctgaa ggtcgcgctg gagctggaaa agatcgcgct
      481 cagcgatgaa tatttcatcg accgcaaact ctatccgaat gtggacttct attcgggcat
      541 cacgctcaag gcgatgggct tccccacctc gatgtttacc gtgctgttcg cggtcgcccg
      601 caccgtcggc tggatcagcc agtggagcga gatgatcgag gatccgcatc agaagatcgg
      661 ccgcccgcgc cagctttaca ccggcgtcac gcatcgcgat tacgtcgata tcgacaagcg
      721 caagtaaatt cgaggctttg aactgtggat gcggccaggc cgcatccgac ttcgtgatcg
      781 ggagacgcga gaatgacgca tcaggccgcc gatagccgca cgtgaaaccg gctgccggtt
      841 gcctcagcgt agcgcaacaa tgtcttggtg gttggcagcg tctgaccgct ctccagtcgg
      901 gcgattgtag actggctggt acccatgcgg gcagccagtt cggcctgcga caacctcgcc
      961 cgcaagcgag ccttcaccaa ctcgaccgaa atctcgaact ccggggcaag cgcctcgtat
     1021 tcgaccttga ccttgggatt ggcgagcaga cgagccttaa gcttttcaaa tgggatagtc
     1081 atgtgatctc ctttgctcgc cgaagtgcaa gctggatctc ggcgggcggc gtcctctgtg
     1141 tcttcttcac gaacgcccga accacgacca ctctccggcc gatcgccgtg acatagaggg
     1201 cacgggctat tccatcacgg cctgtcagcc gcaactccca tagcttcccc tccaaatgct
     1261 tacgtgcggc tcgcccaagc tttccaatcc cgcttggccg atgcgctcgg aaagccgaag
     1321 gaaccgcgcc tgcatgtccg ccggaagggc ggcgatttca gccgcgacgg tatcgttgag
     1381 gatttcaact cgccaagtca cgtcggctta tatctcaatt ttgagataga tggaagtggc
     1441 gaaaaggccc ggtgcctagt tcgatccccg cgccttccgg agcgtcgcca gcacgatgtc
     1501 ggccgcccgg acgctcggcg gctggttgcc ggtcgacatg atcgcgtcga gcttggcaaa
     1561 agccgcgacc tgccgctgcc gtaacggcga gtccgtcagt atgtcgcgca gcgcgggcaa
     1621 cagcttctcc ggcgcgcagt cctcctggat gaattccggc acgacgttct cgccgatcac
     1681 cagatttgcc aaaatcaccg aactggtgcg gatcgcccgc cgcatgattg ctgcctcgac
     1741 tgcgccaacc ttgtaagcgg cgaccatcgg cacgcctgcg atcgcaagct cgagcgtcgc
     1801 ggtacccgat ttgacgaaag ccgcatgagc gatacgaaat gc
//
LOCUS       DASWGM010000266         4796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1822820,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000266 DASWGM010000000
VERSION     DASWGM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822820"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..975
                     /locus_tag="VGA15_07860"
     CDS             319..975
                     /locus_tag="VGA15_07860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9587644.1"
                     /translation="MPKPDLIIFDCDGVLVDSEVLSCRCLSEVLAGYGITLGLDQALD
                     LFLGRSTAAVLEHYEGSAQLIPEKFSAELTARVREAFLAALCPIEGVSSVLAGLQIPH
                     CVASSSDFDRVSLSLSLTGLGPYFDGQLFTSQMVERGKPAPDLFLYAAERMRADPSRT
                     LVIEDSVSGVTAGKAAGMTVWGFVGGSHYQSRDGKAILSDAGADRVFERMADFWRVDR
                     "
     gene            982..1935
                     /locus_tag="VGA15_07865"
     CDS             982..1935
                     /locus_tag="VGA15_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEX9587645.1"
                     /translation="MAASDNEKSRLDEAARAGWLYFIAGHTQDEIAKMLQVSRASAQR
                     LVSLCLAERLITFRLEHPIAACMELAARLKDLFHLVYCEVVPTDPAAPLAAAGIAERA
                     ANVLDSTLRTEKPMIVALGTGRAVRAAVERVSPIDRPNHQIVSLVGNISADGSASFFD
                     TVGRLADRTKARHYPMPLPFLMSSAGERDQMLRIDPIAKVRAVAAKADLRLVGIGQMD
                     QSAQVHVDGFVSREELFEMMRLGAVGELTGWAFDAQGRIIDGGTNRRLTSIPPQVPAE
                     ALTIGAAVGPAKVSAIGAALKGRLINGLITDEVTASAILKP"
     gene            2135..3448
                     /locus_tag="VGA15_07870"
     CDS             2135..3448
                     /locus_tag="VGA15_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9587646.1"
                     /translation="MKHVLGALLGAATLLGSAPSIAQTTLTIATVNNGDMIRMQALTS
                     EFTAKNPDVTVKWVTLEENVLRQRVTTDIATKGGQFDVLTIGTYEVPIWAKKNWLIPL
                     DNLGADYDAKDLLPKISDAVTVSGKLYAAPFYGESSMVMYRTDLFQKAGLTMPEKPTW
                     DFVIDAAKKLTDKTASVYGICLRGKAGWGENMAFLTAMSNSFGARWFDEKWQPQFDKP
                     EWKKTLSTYVDLMKAAGPPGASSNGFNENLALFNAGKCAMWIDATVAASFVTNPKDSK
                     VADKVGFALAPNAGLGKNANWLWAWNLAIPAGSKKVEAAEKFIAWATSKDYTKLVASK
                     EGWANVPPGTRTSLYQNPEYLKVAPFAKLTLASIDAADPNKPTVQPVPYVGVQYAAIP
                     EFQGIGTTVGQQFSAALSGSSTVDAALAAAQSSTEREMKRAGYIK"
     gene            3570..4442
                     /locus_tag="VGA15_07875"
     CDS             3570..4442
                     /locus_tag="VGA15_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9587647.1"
                     /translation="MATQQTQVLGRALLTPAVALLFIWMIVPLAMTIYFSTLHYSLLD
                     PGTESFVGLENYRYFLTDPAFLTSLRNTLVLVGSVLAITIFLGTPLALLLDQPVIGRS
                     VVRLMVIAPFFVMPTVSALVWKNLLMHPVSGLFAWGATLIGATPIDWFTDAPLLAVIL
                     IVAWQWLPFATLILLTALQSQDEEQKEAAEMDGASAFSTFLYLTLPHLARPITVVILI
                     ETIFLLTVFAEIFVTTGGGPGLQTTNIAFLIYSQALIQYDVGNASAGGLVAVVIANIV
                     AFFLVRIIGRNLEA"
     gene            4446..>4796
                     /locus_tag="VGA15_07880"
     CDS             4446..>4796
                     /locus_tag="VGA15_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9587648.1"
                     /translation="MARKVTSRRVWVSTGAAWFFGFLIFLPILWMFLTSFKTELEAFA
                     MPPVFLFFHWTTENYATVQGRSDYIHHALNSILIAGGSTLIAMLIAVPAAWSMAFAPT
                     KRTKDVLLWMLSTKM"
BASE COUNT          887 a         1434 c         1496 g          979 t
ORIGIN      
        1 atgctcatcg cagagcggag cgcggctgcc gaagagggac gcgaccttgt tgctcaaggg
       61 cgtcggcgat cgcgtcatgc gctacgcgct cggtggcatc gctcacgagc cgcatggttc
      121 cttaaagccg gcaagccagc cgtcgacctt gccaaggcag gtcggcgggc gacccggcgt
      181 ttgccgatgc tcccttgccc ggttcgttcg gaatcccttc caaacctacc cattcggccg
      241 tcggtcattt gagccctttg actgggtggt tgcccagatg atgagcgtat gctctgatcg
      301 ggagtacgga aacacgcgat gccgaagccc gatctcatta tttttgactg cgacggcgtg
      361 ctggtcgata gcgaggtgct gagctgtcgc tgcctttcgg aagtcttggc gggatacggc
      421 atcactcttg gtctggatca ggcgctcgat ctgtttcttg gacggagcac ggctgcggtg
      481 ctggagcatt acgaaggatc ggcacagttg attccggaga agttttccgc cgaactgacg
      541 gcaagggtgc gtgaggcgtt tctcgccgca ctttgtccga tcgagggcgt gagttccgtg
      601 ctggcgggct tgcagatccc gcattgcgtc gcctcatcca gcgatttcga tcgggtatcc
      661 ctctcgctct ccctgaccgg gcttgggccg tatttcgacg gtcagctctt tacctcgcag
      721 atggtggaaa ggggcaagcc tgcgcctgat ctgtttcttt acgcggccga aaggatgcgg
      781 gcagatccga gccgcactct cgtgatcgaa gacagcgtga gcggcgtaac ggcgggcaag
      841 gccgccggca tgaccgtgtg gggatttgtc ggaggcagcc actatcaatc gcgtgacggt
      901 aaagcgatcc tctccgatgc gggagctgat cgtgttttcg agcgaatggc ggatttctgg
      961 cgcgtggatc ggtagggttc gatggcagca tccgataacg agaaatcgcg tctggatgaa
     1021 gccgcccgcg ccggctggct ctacttcatt gccggtcaca cccaggatga aatcgccaaa
     1081 atgctccagg tgtcgcgtgc ctcggcccag cgcctggttt cgctatgcct cgccgaacgc
     1141 ctgatcacct ttcggctgga acacccgatc gcggcgtgca tggagttagc cgcgcgcctg
     1201 aaggacttgt ttcatctcgt ctactgcgaa gtggtgccta ctgatcccgc cgcgcccctt
     1261 gcggctgccg ggattgccga gcgggccgcc aacgtcctgg attcgacgtt gcgtaccgag
     1321 aagcccatga tcgtggcgtt gggcacgggc agggcggtgc gtgcggcggt cgagcgcgtt
     1381 tcgccgatcg accgtcccaa tcaccagatc gtctcgctgg tcggaaatat ctcggccgac
     1441 ggctcggcaa gcttcttcga tactgtgggg cggctcgccg atcgcaccaa ggcgcgccac
     1501 tatccgatgc cgctgccatt tctgatgtcg tccgcgggcg aacgcgacca gatgctgcgg
     1561 attgatccga tcgccaaagt gagggcggtc gctgccaaag ccgacctgcg gcttgtcggg
     1621 atcggccaga tggaccagag cgcgcaagtc catgttgacg gcttcgtcag ccgcgaggaa
     1681 ttgtttgaaa tgatgcggct gggcgccgtt ggcgaactga ccggttgggc gtttgatgcc
     1741 cagggacgca tcatcgacgg agggacaaac cgccgtctca ccagcattcc accgcaggtc
     1801 cctgccgagg ccctgaccat cggagcggcc gtcggtcctg ccaaggtctc ggcgatcggg
     1861 gcggcgctga agggacgtct gatcaacggg ttgatcacgg acgaggtcac ggcaagcgcc
     1921 atcctgaagc cgtagcgaca tcgaccgcat attgggcgcc tccagccgag gaaaccgggg
     1981 ctgacgtcga gcggcgggac cagttttcgg tctgccttgc cggctgcaac gcccggtgca
     2041 tgccttcgat cttatcttga caacgttgca gcccgatgtg aacataattg caatgcgtgg
     2101 gcatatgctc aaacgcaaaa atagggaggt taccgtgaaa cacgtcctcg gcgccctgct
     2161 gggcgcggct accctgttgg gctcggcccc ctcgattgca cagacaacat tgacgatcgc
     2221 caccgtgaac aacggcgaca tgatccgcat gcaggcgttg acgagcgagt tcaccgccaa
     2281 gaatcccgac gtgacggtga agtgggtgac gcttgaagag aacgtcctgc gccaacgcgt
     2341 caccaccgat atcgcgacca agggcggtca gttcgatgtg ctcaccatcg gcacctacga
     2401 ggtcccgatc tgggccaaga agaactggct cattccgctc gacaatctcg gggccgatta
     2461 cgacgccaag gatcttctcc ccaagatcag cgatgccgtg acggtgtcgg gcaagctgta
     2521 tgccgcgcca ttctatggcg agagttcgat ggtcatgtac cgcaccgacc tgttccagaa
     2581 ggccggcttg acgatgccgg aaaagccgac ctgggatttt gtgatcgatg cggcgaaaaa
     2641 gctcaccgac aaaaccgcca gcgtgtacgg catctgtctg aggggcaagg cgggctgggg
     2701 cgaaaacatg gcgttcctga cggcgatgtc caattcgttc ggagcgcgct ggttcgacga
     2761 gaaatggcaa ccgcagttcg acaagccgga atggaagaag accttgtcga cctatgtcga
     2821 tctgatgaag gctgcgggtc ctcccggcgc aagctcgaac ggtttcaatg aaaatctggc
     2881 gctgttcaat gccggcaagt gcgcgatgtg gatcgacgcc acggtcgcgg cctccttcgt
     2941 gaccaatccg aaggattcga aggttgccga caaggtcggc tttgcacttg ctccgaatgc
     3001 ggggctcgga aagaacgcca attggctctg ggcgtggaat ctcgccattc cggcgggatc
     3061 gaaaaaggtg gaagccgcgg agaagttcat cgcctgggcc accagtaagg actacaccaa
     3121 gctcgtcgcc tcgaaggagg gatgggccaa tgtgccgccc ggcacgcgaa cctcgctcta
     3181 tcaaaaccct gaatatttga aggtcgctcc gttcgcgaag ctgacgcttg cttcgatcga
     3241 cgcggccgat ccgaacaagc cgaccgtgca gcccgtgcca tatgtcgggg tgcaatacgc
     3301 ggcaattccc gaattccagg gcatcggcac caccgtcggc cagcagttct cggctgcatt
     3361 atcaggctcg tcgaccgtcg atgcggcgct cgccgccgcg caatcgtcga cggaacgcga
     3421 aatgaaacgc gccggctaca tcaaataggg cgaatcgcgt cgaccccagc ggcggtctcc
     3481 ttccgacgag ccgcaaattg gctgccgtcc aacccaatat tggccggacg gcagccctca
     3541 tcgagtttcc cagatagcag gaggcggcga tggcaactca gcaaacacag gtgctcggaa
     3601 gggcgctgct gactccggcc gtcgcgttgc tgttcatctg gatgatcgtc ccgctggcga
     3661 tgacgatcta tttttcgacg cttcattaca gcctgctgga tccgggtacg gagagcttcg
     3721 tcggcctgga gaactaccga tattttctga ccgatcccgc ctttctgaca tcgctgcgga
     3781 acaccctggt gctggtcggt tcggtgctcg ccatcacgat ctttctcggc accccgctgg
     3841 cgctgctgct cgaccagccg gtgatcgggc gcagcgtcgt caggctgatg gtgatcgcgc
     3901 cgtttttcgt gatgccgact gtcagcgcgc tggtctggaa aaacctgctg atgcatccgg
     3961 tctccggact gttcgcgtgg ggcgcgacgc tgatcggtgc gacgccgatc gattggttca
     4021 ccgacgcgcc tcttctcgcg gtgatcctga tcgtcgcttg gcaatggctg ccgtttgcga
     4081 ccctcatcct gctcaccgcg ctgcagtccc aggacgaaga gcagaaggaa gcggccgaga
     4141 tggacggtgc gagcgcgttt tcgacgttcc tctatctcac actgccgcac ctcgcgcgac
     4201 ccattacggt cgtcatcctg attgaaacga tattcctgct gacggtgttc gccgagatct
     4261 tcgtgacgac cggtggcggc cccggcttgc aaacgaccaa tatcgcattc ctgatctact
     4321 cgcaggcgct gatccagtac gacgtcggaa acgcctccgc cggcggattg gttgcggtcg
     4381 tgatcgccaa tatcgtggcc ttcttcctgg tgcggatcat cggacggaac ctggaggcgt
     4441 aagcgatggc acgcaaggta acttcaagac gggtgtgggt gtccaccggt gctgcgtggt
     4501 tcttcgggtt tttgattttc ctgccgatcc tgtggatgtt cctgacaagc ttcaagaccg
     4561 agctcgaggc gttcgccatg ccgccggtgt tcctgttttt ccactggacg acggagaact
     4621 acgccacggt gcaaggccgc agcgactaca ttcaccatgc gctcaattcc atcctcatcg
     4681 ccggcggatc gacgctgatc gccatgctga tcgcggtgcc ggcggcctgg tcgatggcgt
     4741 ttgcgccaac aaagcgcacc aaggacgttc tgctgtggat gctctcgacc aagatg
//
LOCUS       DASWGM010000267         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1822879,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000267 DASWGM010000000
VERSION     DASWGM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822879"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGA15_07885"
     CDS             complement(<1..365)
                     /locus_tag="VGA15_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX9587649.1"
                     /translation="MVWSVRIILVVLALVGATPLHAVQVDEILPDPALETRARAISHD
                     LRCMVCQNQSIDDSEAPLARDLRLLVRERLKAGDSDTQVVDFMVARYGEFVLLRPRMS
                     WHTAILWGAPLTILVVGFFA"
     gene            complement(428..2410)
                     /locus_tag="VGA15_07890"
     CDS             complement(428..2410)
                     /locus_tag="VGA15_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX9587650.1"
                     /translation="MIAELGHYALVLALALALIQSSSPVIGARTRDVALMKLADSTAL
                     AQFAFVALAFAALTICYVTSDFSVANVYENSHSQMPLVYKFTSVWGNHEGSMLLWVLI
                     LAIFGALVALFGANLPATLKANVLAVQSWIASAFYLFILFTSNPFRRLAEVPVEGRDL
                     NPILQDFGLAVHPPMLYLGYVGFSIAFSFAVAALIDGRIDAAWARWVRPWTLLAWMCL
                     TLGIAMGSYWAYYELGWGGWWFWDPVENASLMPWLAGTALLHSAVVMEKRNALKVWTI
                     LLAILTFSLSLIGTFLVRSGVLTSVHTFSSDPARGVFILLILALFIGGSLALFAWRAP
                     LLKQGGLFAPISRESALLFNNLFLATACATVFVGTLYPLVLEALTGDKISVGAPFFNL
                     TFGPLFVPLLMAVPFGPLLAWKRGDLYGVAQRLLAAGGVAAVGLAATFAFAGTKAVFA
                     PFGIGLALFVMAGAITDIAERIDLRSMSLETIGRRAAGLPRSAWGTAVAHFALGITLL
                     GIVCETNWSSERIVALKPTQTVSISGYDLSFDGMVSRDGSNYRETAARFTVRKAGAVI
                     GVMEPSKRTFPSRDGMSTTEAALLTRGASQLYLSLGDNNADGSIAMRLYYKPLVLMIW
                     LGAMVMAMGGALSLSDRRLRIGAPKPAARILAQPAE"
     gene            complement(2448..>2909)
                     /gene="ccmE"
                     /locus_tag="VGA15_07895"
     CDS             complement(2448..>2909)
                     /gene="ccmE"
                     /locus_tag="VGA15_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX9587651.1"
                     /translation="QRRLILIGSSLGVLAFAVALVLSSLRESIVFFNSPTDIAEKKSA
                     AGTRVRLGGMVKVGSLQRGDNLQLRFEVTDGNKDIPVSYRGIVPDLFREGQGVVAEGR
                     IEPGGTFMADTVLAKHDENYMPREVVETLKKQGHWQETAAPNPVPIKASPK"
BASE COUNT          647 a          966 c          871 g          425 t
ORIGIN      
        1 gcaaagaagc cgacgaccaa tatcgtcaac ggcgcccccc acaggatggc cgtatgccag
       61 ctcatccgcg gcctcagcaa gacgaattca ccgtagcggg caaccatgaa atcgaccacc
      121 tgggtgtcgc tgtcgccggc cttgagccgc tcgcgcacga gcaggcgcag atcgcgcgcc
      181 agcggcgcct cggaatcgtc gatcgactgg ttctggcaca ccatgcagcg caggtcgtga
      241 gagatcgcgc gggcgcgcgt ttccagcgcc gggtcgggga gaatctcgtc cacctggacg
      301 gcatgcagcg gcgtcgcgcc cacaagcgcc agcacgacca aaataatccg aacgctccag
      361 accattcgaa gtccttaacc cctgccccag ccggccgcgc cgcaaccacc gcgactgaga
      421 gagaaaacta ttctgcaggc tgcgccaaaa tcctcgccgc cggcttcggc gcaccaatcc
      481 gcaatctgcg gtccgacaac gacagcgcgc cgcccatcgc catcaccatc gcgccgagcc
      541 agatcatcag caccagcggc ttgtaataga gccgcatcgc gatcgacccg tcggcattgt
      601 tgtcgccgag cgacaggtaa agctggctgg caccgcgcgt cagcagcgcg gcctcggtgg
      661 tcgacatgcc atcgcgtgac ggaaaggtgc gtttcgatgg ctccatgacg ccgatcaccg
      721 cgccggcctt gcgcacggta aagcgcgcgg ctgtctcgcg atagttcgac ccatcccggc
      781 tcaccatgcc gtcgaaactc agatcgtagc cgctgatgga aaccgtctgc gtcggtttga
      841 gcgcgacgat acgctcgctg ctccagttgg tctcgcaaac aattccgagc agcgtaatgc
      901 cgagcgcaaa atgcgccacc gcggtccccc aggccgaccg gggcagtccc gccgcacgac
      961 gcccgatggt ctcaagagac atactgcgca gatcgatgcg ttcggcaatg tcggtgatgg
     1021 cgccagccat cacaaaaagg gcaagcccta ttccgaacgg cgcgaacacg gccttggtgc
     1081 cggcgaacgc gaaggtcgcc gcgaggccaa cggccgcaac acctcccgcc gcgagcaaac
     1141 gctgcgcgac cccgtaaaga tcgccgcgct tccatgccag cagaggcccg aacggcaccg
     1201 ccatcagcag cggcacgaac agcggcccga atgtaagatt gaagaacggc gcgccgacgg
     1261 aaatcttgtc cccggtaagc gcctccagta ccaacggata aagtgtgccg acgaacaccg
     1321 tggcgcaagc ggtcgcgaga aacaggttgt tgaacagcag cgcactttcg cgcgagatcg
     1381 gcgcaaaaag gccgccctgc ttgagcagcg gcgcgcgcca ggcaaaaagc gcgaggctgc
     1441 caccgataaa cagcgcaagg atcaacagga tgaaaacgcc gcgcgctgga tcgctcgaga
     1501 aagtgtggac cgacgtaagc actcccgagc gcaccaggaa ggtgccgatc agcgacagcg
     1561 agaaggtgag aatggccagc agaatggtcc acaccttcag cgcattccgc ttttccatca
     1621 ccaccgccga atgcagcaat gcagtgccgg cgagccacgg catcagcgag gcgttctcca
     1681 ccggatccca gaaccaccag ccgccccagc cgagttcgta ataggcccaa taggagccca
     1741 tcgcgatgcc cagcgtcagg cacatccagg cgagcagcgt ccacggccgg acccagcgtg
     1801 cccacgccgc atcgatgcgg ccatcgatca gcgccgccac ggcaaaggag aatgcaatgg
     1861 agaaaccgac atagccgagg tagagcatcg gaggatgcac ggcgagaccg aaatcctgca
     1921 ggatcggatt gagatcgcgg ccctcgaccg gcacctcggc cagccgtcga aacggattcg
     1981 aagtgaagag gataaaaagg tagaaggcag acgcgatcca ggactgcacc gcaagcacgt
     2041 tggccttcag ggtcgccggc agattggcgc cgaacaacgc gaccaatgcg ccgaatatcg
     2101 cgaggatcag cacccacaac agcatggagc cctcgtgatt gccccaaacg ctggtgaatt
     2161 tgtagaccag cggcatctgc gaatgcgaat tctcgtagac attggcgacc gagaaatccg
     2221 acgtcacata gcagatggtg agcgcggcaa acgccagcgc aacgaaggca aattgcgcca
     2281 gcgcggtgga gtcggcgagt ttcatcagcg caacgtcgcg cgtgcgcgcg ccgatgaccg
     2341 gcgacgacga ttggatcagc gccaatgcaa gcgccagcac cagcgcgtag tgaccaagtt
     2401 cggcgatcat ggcgatggct cccagttcac gtccggcgtc tcgccgctca ctttggactt
     2461 gccttgatcg ggacagggtt tggtgccgcg gtctcctgcc agtgtccctg cttcttcaag
     2521 gtttcgacca cctcccgcgg catgtaattc tcgtcatgct tggcgagcac ggtatccgcc
     2581 ataaaggttc ctcccggctc gatgcgtccc tcggcgacaa cgccctgccc ttcgcgaaac
     2641 aggtcgggca cgatgccgcg gtagctgacc ggaatgtcct tgttgccatc ggtcacctca
     2701 aaacggagct gcagattgtc gccgcgctgc aggctgccga ccttcaccat gccgccgagg
     2761 cggacgcgcg tgcctgccgc ggactttttc tcggcgatgt cggtcggcga attgaaaaac
     2821 acgatcgact cgcgcagcga gctcagcacc agcgccaccg cgaaggccag cacgccgaga
     2881 cttgagccaa tcaagatgag gcgccgctg
//
LOCUS       DASWGM010000268         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2242655,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000268 DASWGM010000000
VERSION     DASWGM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242655"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1691)
                     /locus_tag="VGA15_07900"
     CDS             complement(<1..1691)
                     /locus_tag="VGA15_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HEX9587652.1"
                     /translation="MTGHRKTPAKRQAGKEILQFAPNFTAYVLPPDVVCLYSEDRKFF
                     LHGELYCALASAIGKNGRSLRELVGQLELDFPSDKINEALKRLLERRYVVSLSSSSAS
                     TVAGYWSSLGLPPEMAEKNLANCRVRIQSIDVQGAAELGAALSELGVRVVKSSPDLTV
                     TLVNDYLERQLAELNRQRVSDKTPWLLVQPSGAFPLVGPVFIPGESACWTCLFDRMIR
                     NREVKGFLDRGPARPVAVSSLVRNAVGQSGIQLAALEIAKAIATGFRTELRDHIVSLD
                     LPGATIAKHYVAARPQCPTCGRKKLRDPRRAPVPIEPGPGAKLVMTSGGYRTVSSRAT
                     VARFRKHVSPLTGVVTRLERIEADLPMNTNFFAQHNFSAPARNVDELRAGLSGGSFGK
                     GSTAEQGEASALMEAIERYSGIYQSDEIRLTRRFTDFAPGDAILPNDVLLFSEAQSQP
                     DETQTDDSHQQMIPARFDQSAKIEWSPVWSLRDQRFRYLPTSILYFFYSGPAAFHADS
                     NGCAAGNTLEEAIVQGFLELVERDSYAIWWYNRLQRPEVDLSQFDDSYVRDLQTQL"
     gene            2666..>3521
                     /locus_tag="VGA15_07905"
     CDS             2666..>3521
                     /locus_tag="VGA15_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9587653.1"
                     /translation="MPEKKPSRLDAALRLYVGSNGRTDMVDYHGRFAWYELMTTDMAA
                     AKAYYAEVVGWGTQDASTPELAYTLFTTGRASVSGLMDLPEEARKMGATPRWMGYVGV
                     NDVDAAADRIKRLGGAVYVPPTNSNIGRISVVADPQTATLALVKGLKPGQRRPDEFGK
                     PGRVGWHELLAADWQKAFAFYGELFDWQRADAEIDTANTYQLFSAGGQTIGGMFTKHE
                     FEPIPFWLFYFNIDDIDAAAERVTTGGGHIFEGPVELPGGIWIARCRDPQGAAFALQG
                     RRSQQGIAR"
BASE COUNT          674 a         1067 c         1082 g          698 t
ORIGIN      
        1 agctgggttt gcagatcgcg aacgtaggaa tcgtcgaatt ggctgaggtc cacttccggg
       61 cgttgcaatc ggttgtacca ccagattgca tatgaatccc gttccaccag ttcgaggaat
      121 ccctgcacga tcgcttcctc gagcgtgttg ccggcggcgc agccgttgga atccgcgtgg
      181 aaggcggccg gaccgctgta gaaaaaatac aatatgctgg tcggcagata tctgaagcgc
      241 tggtcgcgca gcgaccagac cggcgaccac tcgatcttgg ccgattgatc gaagcgagcc
      301 gggatcattt gctggtgtga atcgtccgtt tgcgtttcgt ctggctggct ctgcgcctcg
      361 ctgaacagca agacgtcgtt gggaagaatg gcatcgcccg gcgcaaaatc cgtgaaccgc
      421 cgtgtcaatc tgatctcgtc gctttgataa attccggaat aacgctcgat cgcctccatc
      481 agcgcgctgg cttcaccctg ctcggcggta gagcccttgc cgaagctgcc gccgctcagt
      541 cccgccctga gctcatcgac gttccgggcc ggcgccgaaa aattgtgctg ggcaaagaag
      601 ttggtgttca tgggcaaatc ggcttcgatc cgctcgagcc gcgtgaccac gccggtgaga
      661 gggctcacat gctttcggaa acgtgccacg gtcgcccgcg acgacacggt ccggtatccc
      721 ccgctcgtca tcaccagctt ggcgccgggg ccaggctcga tcggcaccgg cgcccggcgc
      781 gggtcccgca gcttcttgcg gccgcaggtt ggacattgcg ggcgggccgc cacgtaatgc
      841 tttgcgatgg tcgcgcccgg caggtcgagg ctgacaatgt gatcgcgcag ctcggtgcga
      901 aaaccagtgg cgattgcttt cgcgatttcg agggccgcaa gctggatgcc gctttgcccg
      961 accgcgttgc ggacgagcga tgaaacggcg accgggcgcg ccggtccgcg gtcgagaaac
     1021 cccttgacct ccctgttccg tatcatgcga tcgaaaagac aggtccaaca ggcgctttcg
     1081 cccgggatga acacgggtcc caccagggga aaagccccgg agggttgcac gagcagccac
     1141 ggcgttttat ccgatacgcg ctgccggttc agctcggcca gttgccgttc gagatagtcg
     1201 ttcacgagcg tgacggtcag gtcgggagaa ctcttgacga cgcgaacgcc aagttcgctc
     1261 agcgccgcac cgagttcggc ggcaccctgc acgtcgatcg attgaatgcg cacacgacaa
     1321 ttcgcgagat ttttctcggc catctccggc ggcagaccga gactagacca gtagccggcc
     1381 acggtgcttg cggaagaact cgacagcgac acgacatagc ggcgctcgag caaccgcttg
     1441 agcgcttcgt tgattttgtc ggatggaaaa tccagctcca gctggccaac cagctcgcga
     1501 aggcttcttc cgttttttcc gattgcggag gcgagggcac aatagagctc gccgtgaagg
     1561 aagaacttcc ggtcctcgga atagaggcac acgacatcgg ggggcagcac ataggctgtg
     1621 aaattcggcg cgaactgcag aatttctttg ccggcttgcc gcttggcggg ggtcttgcga
     1681 tggcctgtca ttcggtcatt acacgcatgt tgcttgccac tccggatcgc ccttgcgagg
     1741 cgcactctcc gggcaccgga gctccctggt ccagcgcgcg ctggccgttc tggattgggt
     1801 tgaagatcag ctcggccgtc atgcgcgcgt ctggctagcc acacatttgg aggaccgcca
     1861 agaagacacg aattggccgg gtcgacctcg tcgaacccgg ccatgacgcc ttgcgtttgt
     1921 caatgtattc ggaacgcggc ctagcctagc acaggcggca agcgccccac gacaggcagc
     1981 agcctccgca gccgccgccg caaccacagc caccgccgca tctacaacct ccgcagccgc
     2041 ggcatcctct gcatcctctg cagcctctgc accctccgca tccgcatccg cgggcttcct
     2101 gtaccatgcc gcgaagcgat cctgtgttct ccttgtcgaa gacatggaac gtcgccaggc
     2161 tgacgtcgga gatttcctct tcaccaagca tgatttcgtg acttggtgca acgttcggca
     2221 cccgcggcac gtcctgtgtc ggcacggccg ctgcggatgc accccccact aatgaaaagg
     2281 tcaatccggc ggctcccaac gccgggacgg ctttcgttcc acgcttgcgc ttcgaagctt
     2341 gcttcgcccg tggcatagtt gcatcctcca tgcattgaat tgcagaaatt tttcgcgtcc
     2401 agcgcggcga ttgtaggctt tgcgtctgcg ccgtgcaaga ccggtaaggc actcaagcgc
     2461 caacgacata tcaagttctc gtagttcagg ttcatgaacg acatattcat aaaacgtgtt
     2521 cataaaacat gttcataaac gacgtgtgcg ccgcatattc atcacaccat ctccatgatc
     2581 ctctattgcc ttgacggtgc gtgcgtcctt gcattcgcgc caccccgcca ttattcaggg
     2641 ccgacgggct tgacctctcg atcatgtgcc ggaaaagaag ccgagtcggc tcgatgctgc
     2701 gctgcggttg tatgttggct cgaatggaag aaccgacatg gtcgactatc acggacgttt
     2761 tgcctggtac gagctgatga cgacagacat ggcggccgcc aaggcctact acgctgaagt
     2821 cgtgggctgg ggcacgcagg acgcgtcaac gcctgagctg gcctatacct tgtttacaac
     2881 cggaagagcc tcggtaagcg gactgatgga cttgccggag gaggcgagga aaatgggcgc
     2941 gacgccgagg tggatggggt atgtcggcgt caacgacgtg gacgccgctg ccgaccggat
     3001 caagcgtctt ggcggcgcgg tatatgtccc accgacgaac agcaacatcg gtcgcatttc
     3061 ggtcgtcgct gatccgcaaa cggcgacgct tgcgttggtc aaggggctga agcccggcca
     3121 acggcggccg gatgagttcg gcaagccggg gcgcgtgggc tggcatgagc tgctcgccgc
     3181 cgactggcag aaggcgtttg ctttttacgg cgagcttttc gactggcaga gagcggacgc
     3241 cgaaatcgat acggcgaaca cgtatcagtt gttctcggcc ggcgggcaga cgatcggcgg
     3301 catgttcacc aaacatgaat tcgagccgat cccattctgg ctcttttatt tcaatatcga
     3361 cgatatcgac gcggccgcgg aacgggtgac gacgggtggc ggccatatct tcgaggggcc
     3421 agttgaattg ccgggcggca tctggatcgc ccgatgcagg gatccccagg gcgccgcgtt
     3481 cgcgctgcag ggaaggcgaa gccagcaggg tatcgctcga g
//
LOCUS       DASWGM010000269         3448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2519130,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000269 DASWGM010000000
VERSION     DASWGM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2519130"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VGA15_07910"
     CDS             <1..1456
                     /locus_tag="VGA15_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9587654.1"
                     /translation="TDSSSASGRSVAWTFQPMAGGGTVVLVEDITERKNAEARISHLA
                     RYDELTALPNRVNFRDEIERLLAISHSAERLSALLFVDLDQFKQVNDTLGHPCGDQLL
                     CAVADRLREMLRPEDFVARFGGDEFVVFQQNILSNEDAAGLAQRIVDRLSERYKIGNH
                     LVEIGASVGIAMTSPAGISADTLLKNADMALYRAKADGRGTFCFFRDEMAQTVEARRI
                     LELDLRKALAHEEFELFYQPLVNLKSGRIATCEALLRWNHPVRGTVSPIDIIPVAEDM
                     GLIVDLGRWILRKACMECMKWPQGVSVAVNFSPQQFHQRDVLSEVRYALEVSGLPAHR
                     LEIEITESSLLRNTQLTHDVLSQLHMIGVRISLDDFGTGYSSLSYLHNFPLQKVKIDR
                     SFLEGIDSDRPLTLLRGVARLSADLGMSVVVEGIETNEQLELISVDGTVTEAQGYLFS
                     RPVPAVRIRQLLNASHGRRLTDERLLELPSRSIA"
     gene            1660..2394
                     /locus_tag="VGA15_07915"
     CDS             1660..2394
                     /locus_tag="VGA15_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587655.1"
                     /translation="MRPAAEAIALVPERTADPLEHVDYRLAETPEDKDRIYRLRYRAY
                     LREGAILPSESERVTDRYDDLPNNFTFGVYVRGELCSSIRISVLTSEYRGSPSSEMFT
                     KLLHPELDRGKVIIDPTRFVADPEKAKTFPELPYVTVRLGYVACGHFNADIGLANVRP
                     EHRAFYRKVFLQEPWGEPKLYPGLIKPVGLVAAHYPSIREKVFQRFPYMRSSAFERRT
                     LFQRRGERDSLSPGAMAQPTSILPRS"
     gene            2741..3232
                     /gene="pal"
                     /locus_tag="VGA15_07920"
     CDS             2741..3232
                     /gene="pal"
                     /locus_tag="VGA15_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX9587656.1"
                     /translation="MKHQMRILQGLKLAAVLAVALSMGACANKNLGADGAMASAATPG
                     SQQDFVVNVGDRVFFESDQTDLSPQAIATLEKQAQWLQSYNRYSFTIEGHADERGTRE
                     YNIALGARRAQSVRTYLASRGIDPGRMRTISYGKERPVAVCNDISCWSQNRRAVTVLN
                     ASS"
BASE COUNT          638 a         1040 c         1059 g          711 t
ORIGIN      
        1 caccgactcc agttccgcga gcggccggtc ggtggcctgg acgtttcaac cgatggccgg
       61 cggcggcacc gttgtgctgg tggaagacat caccgagcgg aagaatgcgg aagccaggat
      121 cagccatctc gcgcgttacg atgaactgac cgcgctgccc aacagggtca atttccgcga
      181 cgaaatcgag cgcctgctgg cgatttcgca cagcgccgag cggttgtcgg cattgctgtt
      241 tgtcgatctc gatcaattca agcaggtcaa cgacaccttg ggtcacccgt gtggcgacca
      301 gctgttgtgc gccgtcgccg acagattacg cgaaatgctg cgcccggagg atttcgtggc
      361 gcggttcggc ggcgacgaat tcgtggtgtt ccagcagaac atcttgtcca acgaggacgc
      421 cgcgggcctc gcccagcgca ttgtcgatcg cctcagcgag cgttacaaga tcggcaacca
      481 tctggtcgaa atcggcgcca gcgtcggcat cgcgatgacg tcgcccgccg gcatcagcgc
      541 cgatacgctc ttgaagaacg cggacatggc gctctatcgc gccaaggcgg acggccgtgg
      601 caccttctgc ttcttccgcg acgagatggc gcaaaccgtc gaggcccgcc gcattctgga
      661 gttggacctg cgcaaggcgc tggctcatga ggaattcgag ctgttctatc aaccgctcgt
      721 caatctcaag tccgggcgga tcgcgacgtg cgaagcgctg ctgcgatgga atcatccggt
      781 gcggggcacg gtttccccga tcgatatcat tccggtcgcc gaagacatgg gcctcatcgt
      841 cgatcttggc cgctggatcc tgcgcaaggc ctgcatggaa tgcatgaaat ggccgcaagg
      901 cgtcagcgtc gcggtcaatt tttcgccgca gcagttccat cagcgcgatg tgctgagcga
      961 ggttcgttac gcgctcgagg tctcggggtt gccggcgcac cgcctcgaaa tcgaaatcac
     1021 cgaatcgtcg ctgttgcgca acacgcaatt gacccacgac gtgctgtcgc aattgcacat
     1081 gatcggcgtg cgaatatcgc tggacgattt cggcaccggc tattcgagcc tgagttatct
     1141 gcataatttt ccgctgcaga aagtgaagat cgatcgttcc ttcctggaag gcatcgatag
     1201 cgatcgtccg ctgacgctgt tgcgcggcgt ggcgcggctc agtgccgatc tcggaatgtc
     1261 ggtcgtggtc gaaggcatcg agaccaatga gcagctcgag ctgatcagcg tcgacggcac
     1321 ggtgaccgag gcgcagggct atttgttcag ccggccggtg ccggcggtgc ggatacgcca
     1381 gttgctgaac gcttcgcacg gccgccgcct gacggacgag aggttgctcg aattgccgtc
     1441 gcgatcgatc gcctagaaaa taaaataacg acaattcgtc ggtgtgattt tccgcgccgg
     1501 tctggcgcgc gtcgcggaat ttgagccgtc ggattaaccc tatcgccgcg atccctttgc
     1561 atcggtgtta ataagctgtt aacattcttc cctgcccgca taagttgttg ataagtcgta
     1621 acgcgcggaa gttgttggga agtttgtaag gagtagcata tgaggccggc ggccgaagca
     1681 atcgcccttg tccctgaacg caccgccgat cccctcgaac atgtagacta ccgtctcgcc
     1741 gaaacgcctg aggacaagga caggatttat cggctgcgct accgtgcgta tctccgtgag
     1801 ggcgcgatcc tgccatcgga atccgagcgc gtcaccgatc gttacgatga cctgcccaat
     1861 aactttacat tcggggtgta tgttcggggc gagctttgca gttcgattcg catcagcgtc
     1921 ctgacctcgg agtaccgggg atctccctcg tctgaaatgt ttaccaagct tctgcatccg
     1981 gagctcgacc ggggcaaggt cattatcgat ccgacccgct tcgtcgcgga tcccgagaaa
     2041 gcgaaaacgt ttcccgagct tccctatgtc acggtgcggc tgggctatgt cgcgtgcggc
     2101 catttcaatg ccgatatcgg gcttgccaac gtccgccccg aacatcgggc cttctaccgc
     2161 aaggtctttc tgcaagagcc ctggggcgaa ccgaaattgt acccgggcct gatcaagccg
     2221 gtcggactgg tggccgccca ttatccgagc attcgggaaa aggtgttcca gcgcttcccc
     2281 tatatgcgtt ccagcgcgtt cgagcgacgg acgttgtttc agcgccgcgg cgagcgcgat
     2341 tcattgtcgc cgggcgcgat ggcccagccc acctcgatcc tcccgcggtc ctgacgaatc
     2401 ggggcggcgc cacgtcctgt ccagttttgc gccaataggc gctgatggcg gtttccgcgg
     2461 caggttcccg ctcggcaggc gcgcattgcg cgaggcgggc gcgcattgcg cgaaatgggt
     2521 ggatttcgtt gacctgaaaa ggggttatcc ccaccgcgca ttcgcccgcc ttcaccgtcg
     2581 cgccacattt acaactcatt aactatcgcg gttgcttttc ggtatttgct ggcatttgat
     2641 cgggtctggc aacatcccat caaggttgac ggaacgttcg cttaaccatc gccctgtaga
     2701 ccgggtaaca gtcgaaaaac gcgagcgtgg aggctcccga atgaaacatc agatgcgcat
     2761 cctccaggga ttgaagctgg ccgcggtgct cgcggtggcg ctgtcaatgg gcgcctgcgc
     2821 gaacaagaac ctcggcgccg atggcgccat ggcaagtgcc gcgaccccgg gtagccagca
     2881 ggatttcgtg gtcaatgtcg gcgaccgcgt gttcttcgaa agcgaccaga ccgatctgtc
     2941 gccgcaggcg atcgcgaccc tggagaagca ggcgcaatgg ctgcaaagct acaaccgcta
     3001 ttccttcacc attgaaggcc atgcggacga gcgcggcacc cgcgaataca atatcgcgct
     3061 gggtgcgcgg cgtgcccaat cggttcgtac ctacctcgca tcgcgtggca tcgatcccgg
     3121 ccggatgcgg acgatttctt acggcaagga acgcccggtc gcggtgtgta acgacatctc
     3181 gtgctggtcg cagaaccgcc gcgccgtcac ggtgctgaac gccagctcgt aaagggttgc
     3241 tctttcgtca ggtttatcgc cggcgccttc gggcgccggt tttgcttggc ggtcttgctc
     3301 atttgcgtcc gatgaagtcc cggatcacgg ccatggcgcg ttccggcgca tcgggaatca
     3361 ccgcgtgtcg gcaattcgca aaccgttcga accgcacgag atgcgccggc agcgcggctg
     3421 cgatgtcggc ctggctctcg atcgggtg
//
LOCUS       DASWGM010000270         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1688516,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000270 DASWGM010000000
VERSION     DASWGM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688516"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1250)
                     /locus_tag="VGA15_07925"
     CDS             complement(<1..1250)
                     /locus_tag="VGA15_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX9587657.1"
                     /translation="MFGHPKGLSFLFATEMWERFSYYGMRALLVLYMVDYLLKPEIAH
                     DVWGLATLKRGLEAVSGPLGIQPLASQIYGLYTGLVYLTPIFGGILADRWIGRTPTVI
                     LGAMLMVAGHFMMAFEHLFLFALVLLILGNGAFKPNISTQVGDLYAPDDLRRDRAYSV
                     FYVGINVGAFFSPLVCGSLGENVGWHYGFASAGVGMAIGLATYLAGLPRLPKQQFQDP
                     PVEAGKPANPDLRKSLLGLAILLIPSTLFWAAYEQQGNTIALWIEHSTDRRLNLLLWQ
                     GEIPVTWFQAINPLMIFAFTPPLIACWSRWAKVGREPSTIGKMSIGCVLLALAYLVMA
                     AAAWSSASASASSLWLLLYFAVLTIGELYFSPIGLSLVSNVASNKSRSTLMGIWFTTS
                     FVGNIAAGWIGSFWSSLSNTSFFLL"
     gene            complement(1451..2119)
                     /locus_tag="VGA15_07930"
     CDS             complement(1451..2119)
                     /locus_tag="VGA15_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9587658.1"
                     /translation="MMAARSTSKAGPDAADEPPARERILDAAFSAFMESGFAEASTLE
                     IATRARVSKRELYALVGSKQDMLVACIAARAKRLQAPADLPEPRDRETLAQVLTAFGA
                     QLLRETSDPTVIAVFRLAIAEAARAPEVARALTSVGIETSRAALRDIMSRAQSAGLLG
                     GQPAEMTEHFAGLLWGNLMMNLLLRTVDRPDPREIMRRAHDATAAFLKIYPRPDKAEM
                     GRSA"
     gene            2215..3132
                     /locus_tag="VGA15_07935"
     CDS             2215..3132
                     /locus_tag="VGA15_07935"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEX9587659.1"
                     /translation="MAKPVLVTSAAGGRQGKTGRHVSEMLLKRGIPVRAFVHKIDERS
                     EALRAQGAEIFEGDFLDVRSVKRAVEGTSSIYFAYPVQDGLLDATAAMAVAAREAGIS
                     RLVNLVMLQSSVDAPTPRMRQNYLSEQVFEWAGIGAVHVRATVFYENLGSQVRLSLPA
                     QGAVRLPWGKEQTVLPLVAGEDVARVAIGLLTSPSLTAGTAYPVVGTVISLKDIIATF
                     GRVLAKDVRYEEISDDEWRRDALARGYNTHALEHLSALWKALRAAGLDPQTARFAVTD
                     TIEKLGGAKPKSFEAFVREGQGELLAKTA"
     gene            3297..4295
                     /locus_tag="VGA15_07940"
     CDS             3297..4295
                     /locus_tag="VGA15_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587660.1"
                     /translation="MSEPTQRMIESNGIRLNIAELGKGPLVLLCHGFPESWYSWRHQM
                     DALAAAGFHAVAPDMRGYGKSDRPEAIDQYTMFHLVGDLVGLLDALKAPTAVVVGHDW
                     GAGVAWQAARLRPDRFRAVACLSVPFRPRGKARPTSVMPRTADAQFYQLYFQEPGVAE
                     AELERDPRATVRNMLFGGSGEGAAAIRAAAASGGTAPSIGMVPHGGGFLRGAGAPAML
                     PAWLSEADIDFYAGEFKRSGFRGPLNYYRNIDRNWELLAAFEGVPVTVPALYVAGDRD
                     MVVSFPGTDQLLANFKHFVPALRNILMLPGCGHWTQQERPSEVNAAIIDFIRGLPN"
     gene            complement(4632..4949)
                     /locus_tag="VGA15_07945"
     CDS             complement(4632..4949)
                     /locus_tag="VGA15_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587661.1"
                     /translation="MNAASHHAGWCHWKLGEFGKMEAAACSAIELYSDAPAQWIELTC
                     ALGLQGKTAEAKAAAKALMKLSPTFTPDRFFEIARQFYGTRVAGPVKADYKALCSTLH
                     RAM"
     gene            5234..5383
                     /locus_tag="VGA15_07950"
     CDS             5234..5383
                     /locus_tag="VGA15_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587662.1"
                     /translation="MPTFIRMVAKLKEPAEFFGDDFRAVALFCGIGLVLTLIAVSSGV
                     QGVWL"
     gene            5491..>5768
                     /locus_tag="VGA15_07955"
     CDS             5491..>5768
                     /locus_tag="VGA15_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587663.1"
                     /translation="MFKTNRRIFLQSFAAGAGTLAASQSFAAGSDGHAAGYDVSAASE
                     YLKTVPRKSGDPVVFTASLDKGAIKATSGGWAREITTRGLPIATDIAGA"
BASE COUNT         1138 a         1792 c         1771 g         1067 t
ORIGIN      
        1 agcaggaaga agctcgtgtt cgacaagctg gaccagaagc tgccgatcca gccggcggcg
       61 atatttccaa caaagctcgt cgtgaaccag atccccatca acgtcgagcg cgacttgttg
      121 gacgccacgt tcgataccag cgagaggccg atcggcgaaa aatagagttc gccgatcgtg
      181 agcacggcga aatagagcag cagccacagc gaactggctg aagcggacgc gctggaccac
      241 gcggcggcgg ccatcaccag ataagccagc gcaagcaaga cgcagccgat cgacattttc
      301 ccgatcgtcg acggttcgcg cccgaccttc gcccatcgcg accagcacgc gatcaatggc
      361 ggcgtgaaag cgaagatcat caagggattg atggcctgga accaggtcac cggaatctcg
      421 ccctgccata atagcaagtt cagccggcga tcggtcgagt gctcgatcca aagcgcgatg
      481 gtgtttccct gctgttcgta ggcggcccag aacaacgtgg agggaatgag caggatcgcc
      541 aagccgagaa gcgacttcct caggtccgga ttcgccggtt tcccggcttc gacaggcgga
      601 tcttgaaact gttgtttggg caggcgggga aggccggcca ggtaagtcgc aagcccgatc
      661 gccatcccaa cgcccgcgct ggcaaagccg tagtgccaac cgacgttttc gccgagagaa
      721 ccgcagacca gcggtgaaaa gaacgcaccg acgttgattc cgacgtagaa aaccgaataa
      781 gcgcggtctc tgcgcagatc gtcgggtgca taaagatcgc ccacctgcgt cgaaatattc
      841 ggcttgaaag cgccgttgcc cagaatgaga agcaccagcg cgaacaggaa aaggtgctcg
      901 aaggccatca tgaaatggcc ggcgaccatc agcatcgcgc caaggatgac ggttggggtt
      961 cggccgatcc atcggtcggc gagaattccg ccgaatatcg gggtcagata gacgaggccg
     1021 gtgtagagcc cataaatctg cgatgccagc ggctggatgc caagcggtcc ggataccgct
     1081 tcgagtcccc gcttcaaggt cgcgaggccc catacgtcgt gagcgatttc cggcttcaac
     1141 agataatcga ccatgtacag aacgagcagc gcccgcattc cgtaatagga aaagcgttcc
     1201 cacatctccg ttgcgaacag aaacgacaga ccctttgggt ggccaaacaa ctgatcgccg
     1261 cgttcgccgc cgcgaaccgg atcgctcgat gttggatcgg tctgagctat gactctctcc
     1321 cattcggcga tttagtcgct atcgccgcca gtcctgggat cgcagtccgc agcgacacct
     1381 tcagcgcctt cgatccaatt ggtgctgggg agtttatcac cgggcgcgga ttcgcgcgac
     1441 ccgttggcgg ttacgcgcta cgccccatct cggctttgtc cggtcgcgga tagattttca
     1501 ggaaggccgc ggtggcgtcg tgggcacgcc gcatgatctc gcgggggtca ggccgatcga
     1561 cggtgcgaag cagcaggttc atcatcaggt tgccccacaa tagcccggca aaatgctcag
     1621 tcatctctgc cggctggccg ccgagaagtc cggccgactg ggccctgctc atgatgtctc
     1681 tcaaggcggc gcggctggtc tctattccga ccgaggtcag cgcccgcgcg acctcgggcg
     1741 cgcgggccgc ttcagcgatc gccaaccgga ataccgcgat gacggtggga tcactggttt
     1801 cgcgcagcag ctgcgcgccg aacgccgtca gcacctgcgc gagggtctcc cggtcacgcg
     1861 gctccggcag gtcggcaggt gcctgcagcc gtttggcgcg cgcggcgatg cacgccacca
     1921 gcatatcctg tttgctgccg accagcgcat aaagctcgcg cttggagacg cgcgcgcggg
     1981 tcgcgatctc aagcgtgctc gcctcggcaa atccgctctc catgaacgcc gaaaaagccg
     2041 catcgaggat gcgctcgcgc gccgggggtt cgtcagccgc atccgggccc gccttcgacg
     2101 tcgatctcgc cgccatcatc tccctcttga cgcggtacca cacggtacca tatgttcaaa
     2161 acaattggta ccgtatagta ccaacccacc gcagaatgta aaggagccat cgccatggca
     2221 aaaccagttc tcgtcacttc agcggccggc ggcaggcaag gcaagaccgg acggcacgtg
     2281 tcggaaatgc tgctcaaacg cggaattccc gtccgcgcct tcgtccacaa gatcgacgaa
     2341 cgctcggagg cgctgcgtgc acaaggtgcc gaaatattcg aaggcgactt cctcgacgtc
     2401 cgctccgtca agcgcgcggt tgagggcacc tcgtcgatct attttgccta tccggtgcag
     2461 gacggcctgc tcgacgcgac cgcggccatg gcggttgcgg cgcgcgaagc gggtatctcc
     2521 cggctcgtta acctggtgat gctgcaatcg tcggtcgatg ccccgacccc gcggatgcga
     2581 cagaactatc tgtccgaaca ggtgtttgaa tgggccggaa tcggcgcagt gcatgtcagg
     2641 gctacggtat tttatgaaaa tctcggctcc caggtgcgct tgagcctgcc ggcgcagggg
     2701 gcagtccggt taccctgggg caaggaacag accgtgcttc cgctggtcgc cggcgaggat
     2761 gtggcgcgcg tcgccatcgg cctcttgacc agcccctcct tgacggcggg caccgcctat
     2821 ccggtggtcg gcaccgtcat ttcgctgaag gatatcatcg caaccttcgg gcgcgtgctg
     2881 gccaaggacg tgcgctatga agaaatttcg gacgacgagt ggcgccgcga cgcgctcgca
     2941 cgcggctaca acacgcacgc gctcgaacat ctctccgcgc tctggaaagc gctccgcgcc
     3001 gccggcctcg atccgcagac cgcacgattc gcggtgaccg atacgatcga aaaactcggc
     3061 ggcgccaagc ccaagtcgtt cgaggcgttc gtgcgcgagg gacagggcga attgttggcg
     3121 aagacagcct gaccaagcca gcaaatggag actgtcatgg ataggcgccg tttacgaagc
     3181 gcggtaggat tgccgctgtt gcgcggcccg cgttgaacgt ccttcattcc aaggcgttcg
     3241 caaacgcctt cgcaaacgcc ttggagcgag aaagaaactt ggagcgagat agaaacatga
     3301 gcgaaccgac ccagcgaatg atcgagagca acggcatccg cctcaacatc gccgagctgg
     3361 gcaaagggcc cttggtgctg ttgtgccatg gctttcccga atcctggtat tcctggcgcc
     3421 atcagatgga tgcgctggcc gcggcgggct tccacgccgt ggcccccgac atgcgcggct
     3481 atggcaagag cgatcggcct gaggcgatcg atcaatacac gatgttccat ctggtcggcg
     3541 acctcgtggg cctgctcgac gcgctcaagg ccccgaccgc cgtcgtcgtt ggccatgact
     3601 ggggcgcggg cgtcgcctgg caagcggcgc gcctgcgccc cgaccgcttc cgcgccgtcg
     3661 cctgcctcag cgtgccgttc cggccgcgcg gcaaggcgcg cccgaccagc gtcatgccgc
     3721 ggaccgcgga cgcccaattc taccagctgt attttcagga acccggcgtc gccgaggccg
     3781 agctggagcg ggatccgcgg gcgaccgtgc gcaacatgct gttcggcggg tcgggcgaag
     3841 gcgctgctgc aatccgcgcc gccgcggcga gcggtgggac cgctccaagc atcggcatgg
     3901 tgccgcacgg aggtggtttc ttgcggggag ccggggcgcc ggcgatgctg ccggcgtggc
     3961 ttagtgaagc cgacatcgat ttctacgccg gcgaattcaa gcgctcaggc ttccgcggtc
     4021 cgctcaatta ttaccgcaac atcgaccgca actgggagct gctggcggct ttcgaaggcg
     4081 ttccggtgac ggtcccggca ctctatgtcg ccggtgaccg cgacatggtg gtctcgtttc
     4141 ccggcacgga ccagctcctc gccaacttca aacatttcgt ccccgcactg cgcaacatat
     4201 tgatgctccc cggctgcggc cactggactc agcaggagcg ccccagcgag gtcaacgccg
     4261 cgatcatcga tttcatccgc ggcctgccga actgaagatg gcggtcgcgc ttcagcgaag
     4321 ccgttataaa gcgtgtcggg ccgatctggg cctttgatga cgagaggcaa gaacttcgcg
     4381 acatgtgggt ccgcacacca cagccgggtc tatggtttac cggcggcgtc ttttctcagt
     4441 gccgcgtcta cagctgctac attgcgttgc agatcgacgc catcgaagcg ggaaagcttg
     4501 caaaagagct gatagctctg agcgacggcg ccaaacccaa gactttcgag gcgtcccaac
     4561 gcaaacgaaa acccacttgg aactgtgccg aaactgcctc ttcgcgacag cgctgctcta
     4621 tggttcatcg cctacatcgc gcgatgcagc gtcgagcaga gggccttgta gtcggccttg
     4681 acggggcctg cgacgcgcgt gccgtaaaat tgccgcgcga tttcgaagaa cctgtcgggc
     4741 gtgaaggttg gcgacagttt catcagcgcc ttcgcggcgg cttttgcctc agcggtcttt
     4801 ccctgcagcc cgagggcgca ggtcaactcg atccactgcg cgggagcgtc gctatagagt
     4861 tcgatcgcgc tgcacgccgc cgcttccatt ttgccaaatt cacccagttt ccaatgacac
     4921 cagcctgcat gatgggacgc agcattcatt ttgctcgccg ggcgggggaa atcctgtggt
     4981 cgttgcgctg cgcggtccgg cgaaggagct aatgaacgtt acacctgaaa catcgcaacg
     5041 gtgaacacaa tcaccgtcaa aaccgtcgcg agcattattg acgctgattg acgaagacgc
     5101 ctgacgtctt ccgaattccg ttggtttcga agtgtgaacg cgctcataaa gcaaaaccac
     5161 caggctgata atatgagatc ggagtttgtc gttcgcgccc gctttgccga aacaacaacc
     5221 gtgcaagggt accatgccca cattcatcag aatggtcgcc aaactgaagg agccggcgga
     5281 gttttttggc gatgattttc gtgcggtcgc tctgttctgc ggcatcggat tggtgttgac
     5341 gctgatcgcg gtgagcagcg gggtgcaggg cgtctggctc taggttatcg cgcgtgacgc
     5401 gcgagcaccc gggttttatt ttctcaagct gtttcgcgtt ctgccaggct tcggattttt
     5461 tgaaatcaca acgcatttgg agcgcaaatc atgttcaaga caaatcgccg gatcttcctg
     5521 cagtcatttg ccgccggtgc cggcacactc gccgcctcgc aatcctttgc cgcgggaagc
     5581 gatggccacg ccgcgggata cgatgtctcc gccgcatcgg aatatttgaa gacagttccg
     5641 cgcaaatccg gtgatcccgt tgtcttcacg gcttccctcg acaagggagc gatcaaggca
     5701 acgtccggcg gatgggcgcg tgaaatcacc acgcgcggac ttccgatcgc caccgatatc
     5761 gcgggtgc
//
LOCUS       DASWGM010000271         5251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2100854,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000271 DASWGM010000000
VERSION     DASWGM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100854"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..287)
                     /locus_tag="VGA15_07960"
     CDS             complement(33..287)
                     /locus_tag="VGA15_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587664.1"
                     /translation="MTPSSIRVTLLAGIAAVAMTVPAAASPTASGSIDTRPHAAATQQ
                     YAQDFSHGSRAVDYGYKSKKCKKSDKKCKAKTKPKSETKG"
     gene            605..1705
                     /locus_tag="VGA15_07965"
     CDS             605..1705
                     /locus_tag="VGA15_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9587665.1"
                     /translation="MAFHVALLTGGLAVSVSAQEREPAAVPVVTVSAERKPIAQSLDF
                     VGRVEATNRVEVRARVEGYLEAVLFKEGDLIKEGDPLYRIEQGLFKAAVEQAQGALER
                     SKASKILTVLQLQRAEDLLTKQAGTAVARDQAVAADQQAKGAIMTDEANLQTAKINLG
                     YTEIIAPISGKIGRTNVTKGNVVGPSAGPLATIVSQDPMYVAFPVSQREFLRAREAGR
                     QADVQSIKVRIRFADGTMYDQTGSINFVDVTVDRSTDTVLVRATVANPKGGLIDGQLV
                     RIVVESGAPEEKVVVPQSALIADQQGVYVFVVEGGKAAVKRLKVGGEIGANVVVDQGL
                     SGGEQVIVEGLQGVRPGIPVRAAPLPPALGRS"
     gene            1709..4891
                     /locus_tag="VGA15_07970"
     CDS             1709..4891
                     /locus_tag="VGA15_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX9587666.1"
                     /translation="MLSSLFVDRPRFAIVIAIVTTIAGLLALFSIPIAQYPDIVPPQV
                     SVSTSYPGASGAVVDATIAQPIEAQVVGVDKMIYMKSVSGDDGSYALTASFELGTDPD
                     INTVNVNNRVQVALSSLPQDVQRQGVTVKKRSSALLAVIALYSPKHTHDPLFISNYVT
                     INLLDQIKSTAGVGDAALWGPQDYAMRAWVRTDRLTGLGLTTGDIVNAIKAQNVQAAV
                     GRIGARPISDEQQLQLNIQTQGRLSSADEFANIVVRTNPDGSVLRLRDVARVELGAAN
                     LDRETRLNGGPAAVVAIYQSPGANAIATLQAVRGRFAELEKRFPEDLAWKVTYDPTSF
                     VTATIHEVQKTLVEAFILVVLVVFLFLGSVRATLIPTFAVPVSLIGTFVVLKAVGYSA
                     NSVSLLAIVLAIGIVVDDAIVVVENVERVMEEHPELSPAEATKRAMREITAPIIAITL
                     VLLSVFVPVAFIPGISGELFRQFAVTVAVAMFLSAINALTLSPALCAVLLRPHHGPRR
                     GLIGGVMRSIDRVRDAYGSVVARIVRFSVIGLVMVGVAGFGVVGLSRITPTGFLPEDD
                     QGGLFVVVQLPGGASIVRTADAVRQAEAIVREEQAVADFTSIIGLNFIDNYSQANAAF
                     MVVTLKSFEERNDPSLGAPAVIARLGGKFRQIQGGTVVPLAPPPILGLGSGGGFAYVL
                     EDLRGGDPKALAQVLRGLVVAANQDPQLNRVFSTFSATNPSIYLDIDRDKVQILGVQL
                     NDVFQALQTSLGGFYVNDMNLFGRRWQVQVQAEADDRANVDDIYRINVRNAEGKMIPL
                     RSLLEVRVVVGPPALIRYNNLRAVTVQGSAAAGVSSGQALKAMETVAARTLPPGYAGE
                     WTDTAFQEKRAEGKTTLILGFAVLFAYLFLVALYESWTIPVPVLLSVSIGVLGSFAAM
                     VIEHLTLDLYAQIGMVVLIGLAAKNGILIVEFAKEKRDQGVPLLEAATEGARLRFRPV
                     MMTSFAFILGLLPLVVAVGASQLARRDVGTPVFGGMILASFIGIFAIPPLYVAFQAAR
                     EKLRPSARPKAPSISPEHGAVQPSAPEAAE"
     gene            5114..>5251
                     /locus_tag="VGA15_07975"
     CDS             5114..>5251
                     /locus_tag="VGA15_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002970068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9587667.1"
                     /translation="MLPDYSMRQLLEAGVHFGHQAHRWNPKMAQFIFGTRNNIHIIDL
                     AQ"
BASE COUNT          906 a         1637 c         1663 g         1045 t
ORIGIN      
        1 gcgcccacca aaagagttgc gccggctgct agtcagccct tggtctcgct cttcggtttc
       61 gttttagcct tgcacttctt gtcggacttc ttgcacttct tggacttgta tccgtagtcc
      121 accgcacgac tgccgtggga aaagtcttgc gcatactgct gagtcgcagc cgcgtgggga
      181 cgggtatcta tgctgccgga agcagtcgga gatgctgccg ccgggacagt catcgcgacg
      241 gctgcgattc ccgcgagcag cgtaacgcga atggatgacg gggtcatggg ttcctccctt
      301 tcagtttgag cgccccccgc gagttcgctc ggagggtcga tttatcctta taccacaggt
      361 agccccaagg tgcgagtagc cttgggtgtc attttagctc gggggtggag cgggtcgcga
      421 tcgatctcgc tgatggttaa ccctggacat ttgtttgtgc gccgggtctt ggtcggtcgg
      481 atcacgagag cggccccgaa acttcgcagg tcgcgcttgg gtcctgcctt gccctgatct
      541 acaatgcctg cgttcttctc cacgtaggac agaatgcggg gttgagatgc gcttggcagt
      601 ccggttggcc tttcacgtgg ctctgttgac cggcggactt gccgtctcgg tcagtgcaca
      661 ggagcgcgaa cctgccgccg ttcctgtcgt tacggtcagc gccgaacgca agccgatcgc
      721 gcagtcgctc gacttcgtcg ggcgagtcga ggccaccaat cgcgtcgagg tcagggcgcg
      781 cgtggaaggc tacctcgaag cggtgctgtt caaggaaggc gacctgatca aggaggggga
      841 tccgctctat cggatcgaac agggcctctt caaggccgcc gtcgaacaag cgcagggagc
      901 cctggagcgc agcaaggcat cgaagattct cactgtgctg cagcttcagc gcgccgagga
      961 tctgctaacg aagcaggcgg gcacggcggt ggcacgtgac caggccgttg cggcggatca
     1021 gcaggccaag ggcgccatca tgacggatga ggccaacctg caaaccgcca agatcaacct
     1081 cgggtatacc gagataatcg ccccgatttc cggcaagatc ggacgtacca atgtgaccaa
     1141 gggcaacgtc gtgggcccga gcgccggtcc tctcgccacg atcgtcagcc aggatccgat
     1201 gtacgtcgca ttcccggtaa gccagcgcga attcctgcgc gcccgagaag ccggccgtca
     1261 ggcggacgtc cagagcatca aggtccggat ccgctttgct gacggtacga tgtacgacca
     1321 gacgggtagc atcaatttcg tcgacgtgac ggtggatcgc agcaccgata ccgtcctcgt
     1381 gcgcgccacg gtagcgaacc ccaaaggcgg gctcatcgat ggtcagctgg tgcggatcgt
     1441 ggtggagagc ggtgcccccg aggaaaaggt ggtggttccg cagtcggcct tgatcgccga
     1501 ccaacagggc gtctatgtct tcgtggtcga gggcggcaag gcggcggtga aacggctcaa
     1561 ggtcggcggc gaaatcggtg cgaacgttgt cgtcgatcaa gggctgtccg gcggcgagca
     1621 ggtcatcgtc gagggactgc agggcgtgcg gcccggcatt ccggtgcgag cggctccgtt
     1681 accgccggcg ttgggccgga gttgatcgat gctctcctcc ctcttcgtcg accggccacg
     1741 gtttgccatc gttatcgcca tcgtcaccac gatcgccggt ttgctggcgc tgttctccat
     1801 tcctatcgcg caatatcccg atatcgttcc gcctcaggtc tcggtgagca ccagctatcc
     1861 gggcgcttcc ggagccgtcg tcgacgcgac catcgcccag ccgatcgaag cgcaggtcgt
     1921 gggcgtcgac aagatgatct acatgaagag cgtgagcggc gatgacggca gctacgcgct
     1981 cactgcttcg ttcgagctcg gcaccgatcc cgatatcaac accgtcaatg tcaacaaccg
     2041 cgtccaggtg gctctttcga gcttgccaca ggatgtccag cgccaaggcg tcaccgtcaa
     2101 gaaaaggtcg tcggccctgc tcgccgtcat cgcactctat tcgccaaagc atactcatga
     2161 tcctctgttc atttccaact acgtcaccat caatcttctc gatcagatca agagcacggc
     2221 cggcgtcggc gacgccgcgc tgtggggtcc tcaggattac gccatgcggg cctgggtgcg
     2281 gaccgataga ctgacgggtc ttggactgac gaccggcgac atcgtcaacg cgatcaaggc
     2341 acagaacgtt caagctgccg tcggccgaat cggcgcccgt cccatatccg atgaacagca
     2401 gctgcaacta aacatccaga cgcagggtcg tctcagctcc gccgacgagt tcgcgaacat
     2461 cgttgtgcgc accaatcccg acggatccgt gctgcgcctg cgcgacgtcg cacgggtgga
     2521 actgggggcg gctaacctcg accgcgaaac gcgattgaac ggcggacccg cggccgtggt
     2581 ggccatctat caaagccccg gtgcgaacgc catcgccacg ctgcaagcgg ttcgcggccg
     2641 gttcgccgaa ctggagaaac ggttccccga ggatctggcc tggaaggtca cctacgaccc
     2701 caccagtttc gtcaccgcga ccattcatga ggtgcagaag acccttgtcg aagccttcat
     2761 tctggttgtt ctggtggttt ttctctttct cggcagcgtg cgcgccactt tgataccgac
     2821 ctttgcggtg ccggtgagcc tgatcggcac tttcgtcgtg ctcaaggcgg tcggctattc
     2881 ggccaattcc gtctcgttgc tggccatcgt gctggcgatc ggcatcgtcg tcgacgacgc
     2941 cattgtcgta gtcgagaacg tcgagcgggt gatggaagag catccggagc tgtcgccggc
     3001 ggaggcgacc aaacgggcga tgcgcgaaat caccgcgccg atcattgcca tcaccctggt
     3061 gctgctctcg gtgttcgtgc cggtcgcatt catcccaggc atttccggcg agctgttccg
     3121 gcaatttgca gtgacggtgg cggtggcgat gttcctttcc gccatcaacg cgctgacgct
     3181 gtctccggcg ctttgcgctg tcctgctgcg acctcaccac gggccgcggc gcggtttgat
     3241 cggcggggtc atgcgctcga tcgatcgcgt gcgcgacgcc tatggaagcg tcgttgcccg
     3301 catcgtgcgt ttctcggtca tcggattggt gatggtcggg gttgcgggct tcggggttgt
     3361 cggtctgtcg aggattactc cgacggggtt cctgccggaa gacgaccagg gcgggctgtt
     3421 cgtggtggtg cagctccccg gcggcgcctc gatcgtgcga accgccgatg cggttcgcca
     3481 agccgaagcc atcgtgcgcg aggagcaggc cgtcgcagat ttcacgtcca tcatcgggct
     3541 gaatttcatc gacaactact cgcaagcgaa tgccgccttc atggtggtca cactcaaatc
     3601 gttcgaggag cgcaacgacc cttcgctcgg cgcgcccgcg gtgattgcca ggctcggcgg
     3661 gaagttccgc caaatccaag gggggaccgt cgttcccctc gcgccgccgc ccattcttgg
     3721 cctgggaagc ggcggcggat ttgcctacgt gctggaggat ctgcgcggcg gtgatccgaa
     3781 ggcgctggcc caggtcttgc gcggcctcgt cgtcgcggcg aaccaggatc cgcagctcaa
     3841 tcgggtattc agcacctttt ccgccacgaa tccctcgatc tacctcgata tcgatcgcga
     3901 caaggtgcag atcctcggcg tgcaattgaa cgacgtgttc caggcgctcc agacctcgct
     3961 cggcggcttc tacgtcaacg acatgaacct gttcggccgc agatggcagg tacaggttca
     4021 ggccgaggcc gacgaccggg cgaatgtcga cgacatctac cgcatcaatg tccgaaatgc
     4081 cgagggcaag atgattccgc tccgcagcct gttggaggtg cgcgtcgtcg tcggcccgcc
     4141 ggcactgatc cgctacaaca atctgcgcgc cgttacggtt cagggcagtg cggcggcggg
     4201 ggtatcctcc ggccaggcgc tgaaggcgat ggaaaccgtc gcggcacgca cgctgccgcc
     4261 cggttatgcc ggcgagtgga ccgataccgc tttccaggaa aagcgcgccg agggaaaaac
     4321 taccctcatt ctcgggttcg cggtgttgtt cgcctatctg ttcctggtcg ccctgtacga
     4381 aagctggact attccagttc cggtgttgct gtcggtatcg atcggcgttc tcggctcctt
     4441 tgccgccatg gtcatcgaac acctgaccct cgatctctat gcgcagatcg gaatggtggt
     4501 gctgatcgga ctcgccgcga aaaacggcat actgatcgtc gagttcgcca aggagaagcg
     4561 ggaccagggc gtgccgcttc tcgaggcagc cacggagggt gcacgtctgc gcttccggcc
     4621 ggtcatgatg acgtcgttcg ccttcattct gggcctcttg ccgctggtcg tggccgtcgg
     4681 cgcgtcacag cttgcgcggc gcgacgtcgg cacgccggtg ttcggcggca tgatcctcgc
     4741 gtcgttcatc gggattttcg ccatcccgcc gctctatgtc gccttccagg cggctcggga
     4801 gaagctgaga ccgtccgctc ggccgaaggc gccctcgata tctcccgagc atggagcggt
     4861 gcagccctcc gcaccggaag cagcggaata acgctggagg ctgcgaagca ggaagagcag
     4921 ggagcatcgc gctgtcgacg cggtgcggga attccttgct cgatccgcgg atgcttgcat
     4981 ccgccattgc gcgccgctat aagcgcgccc atcccacacg cggaccgagg cctttggcca
     5041 ttccggtggt tcccgccgat gccaagtcgg ggaggaactt acgggtccgc ggcggttcga
     5101 accggagaat gccatgctgc ccgactattc gatgcgccag ctgttggagg ctggcgtcca
     5161 tttcggccat caggcccacc gctggaaccc gaagatggcg caattcatct tcggaacccg
     5221 caacaacatc cacatcatcg acctcgccca g
//
LOCUS       DASWGM010000272         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2382132,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000272 DASWGM010000000
VERSION     DASWGM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382132"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGA15_07980"
     CDS             <1..267
                     /locus_tag="VGA15_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX9587668.1"
                     /translation="SVARKHGIVTGLLFRWRVQFGVTQKKRAKLAPVMLVDSTPAALL
                     LRDLVQPPDGMTAVDLADGRRVFAPVGSDPDIVRARVESGEIAP"
     gene            264..617
                     /gene="tnpB"
                     /locus_tag="VGA15_07985"
     CDS             264..617
                     /gene="tnpB"
                     /locus_tag="VGA15_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX9587669.1"
                     /translation="MMIVPAGVKVHLALGFTDMRKGLDGLAMLVQEALKKDPFSGHLF
                     AFRGKKASMLKILFWDGNGLCLFTKRIDQGGFVWPTMTGFDGSITLTPAQLAMLIEGI
                     DWRLPERVWRPAIAG"
     gene            690..>1675
                     /locus_tag="VGA15_07990"
     CDS             690..>1675
                     /locus_tag="VGA15_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9587670.1"
                     /translation="MRFELENLPSDTALLHRLVREMAVVVDSRDGEIERLQSIIKKLQ
                     RAQFGRRSERLDADQLALALEDLDADIARIRESRPAIATDTLEARSRRKPLPDHLPRE
                     DVLLDVSGDICGCCGGALHAIGESISEMLDWVPAQLRVVRITRPKYACRSCNKVVQAV
                     APERLIAGGLATPALLAQVLVSKYCDHTPLYRQSQIFARHDVDLPRSTLVGWVGGACW
                     WLETLHERLCKNVFASDHLFADDTPIPVLDPGRGRTKTGRLWVYAREQRPWAGPEPPA
                     AVYLFAPDRKAERPVAHLERFKGVLHVDGYAGFERLTGNGDIILAACWAHTRR"
BASE COUNT          308 a          492 c          553 g          322 t
ORIGIN      
        1 agcgttgcac gcaagcacgg gatcgtcacc gggttgctgt ttcgctggcg cgtgcagttc
       61 ggcgtgacgc agaagaagcg cgcaaagctc gcccccgtta tgctggtgga cagcacgccg
      121 gcggcgctcc ttctgcggga ccttgtacag ccgcctgacg ggatgacggc tgtcgatctg
      181 gcagacgggc gccgcgtctt cgcgcccgtg ggtagcgatc ccgatatcgt ccgggcgcgt
      241 gtcgagagcg gggagatcgc gccatgatga tcgttccggc tggtgtgaag gtgcatctgg
      301 cgttgggctt caccgacatg aggaagggcc tggacggact cgctatgctg gttcaggagg
      361 cgctcaagaa agatccattc tcgggtcatc tgtttgcctt ccgaggcaag aaggcgtcga
      421 tgttgaagat cctgttttgg gacggaaacg ggctttgcct gttcaccaag aggatcgacc
      481 agggcggatt tgtctggccg acaatgacag gcttcgatgg ctcgatcacg ctgacgccag
      541 cgcaactcgc gatgctgatc gagggtatcg attggcgtct gccggaacgg gtttggagac
      601 cggcgatcgc cggctgaaac accgcataaa cccgcataaa atatagcgaa cttgcttcct
      661 cgaatcagcc gattcgagta gaatcgcaca tgcggtttga gcttgaaaat ctgccgtccg
      721 acacggcttt gctgcatcgt ctggtgcgcg agatggctgt tgtcgtcgac agccgcgacg
      781 gtgagatcga gcgcctccaa tccatcatca agaagctgca gcgagcgcag ttcgggcgtc
      841 gctccgaacg ccttgatgcc gatcagctgg cgctcgcgct tgaagacctt gacgccgaca
      901 ttgcccgcat ccgggagagc cgtccggcca tcgcaacaga cacgctcgaa gcgcggtcgc
      961 ggcgcaaacc gctgcctgat catctgcccc gcgaggatgt tttgctcgac gtcagcggcg
     1021 acatctgcgg ctgttgtggc ggtgcgctcc atgcgatcgg cgaaagcatc agcgagatgc
     1081 tggactgggt gccggcgcag ttgcgcgtcg tgcgcatcac ccgcccgaaa tacgcctgcc
     1141 gcagctgcaa caaggtcgtg caagcggtgg cgcctgaacg actgatcgcc ggcggattgg
     1201 ccacgccagc gctgctcgca caggtgctgg tcagtaaata ttgcgatcac actccgcttt
     1261 atcggcagtc gcagatcttc gcccgccacg acgtcgatct gccacgctcg acgttggtcg
     1321 gatgggtggg cggcgcatgc tggtggctgg aaacgttgca cgagcgattg tgcaaaaacg
     1381 tattcgcctc cgaccatctg ttcgccgacg acacgccgat tccggtgctt gatcccggcc
     1441 gcggacgaac gaaaacggga cggctgtggg tctatgcccg tgagcaacgg ccgtgggccg
     1501 gaccggagcc gccggcagcg gtctacctgt tcgcgccgga ccggaaagcg gagcgaccgg
     1561 tggcgcatct cgaacggttc aagggcgtcc tgcacgttga tggttacgcg ggattcgagc
     1621 ggctgaccgg caatggagat atcattctcg ccgcctgttg ggcgcataca agacg
//
LOCUS       DASWGM010000273         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2243404,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000273 DASWGM010000000
VERSION     DASWGM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243404"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="VGA15_07995"
     CDS             <1..1565
                     /locus_tag="VGA15_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9587671.1"
                     /translation="TAEAEAARAVAQISSTEAIIASLRLEIEKLRREIYGQRSERKAR
                     LLAQMEFQLEELEATASADELTAEQAAAKTTAVKAFTRQRSSRKPFPAHLPRERVVVP
                     APSACACCGSTKLAKLGETITETLESIPRQWKVIQTVREKFTCRECEKITQPPAPFHT
                     IPRGWAGPSLLAMILFEKFGQHQPLNRQCERFAREGVEVSLSTLADQVGTCAGVLKPL
                     YSLIETHVLAAERLHGDDTTVSVLAKGKTITGRLWTYVRDDRPFGGPAPPAAVFYYSR
                     DRTGEHPQRHLHTYAGIFQADAYGGYNKLYEEGRQPSLVLEAACWVHARRRFFVLADL
                     AASARRKAQGKTPAVISPIALEAVCRIDALFDIERQINGQDAGQRRAVRQELSAPLVA
                     ELEAWMRQERARLSRHNDVAQAMDYMLKRWAAFARFLDDGRICLSNNAAERAVRGIAL
                     GRRAWLFAGSDRGGERAAFMYSLIVTAKMNNIDPQAWLADVLCHIADHPVHRLDELLP
                     WNWKRQSAKLAA"
BASE COUNT          367 a          599 c          587 g          316 t
ORIGIN      
        1 ctaccgcgga ggccgaggcg gcacgggccg tggcacaaat ctccagcacc gaagccatca
       61 tcgcgtcact ccggctggag atcgagaagc tgcgtcggga gatctacggc cagcgctccg
      121 aacgcaaggc ccggctgctg gcgcagatgg aattccagct cgaggaactc gaggccaccg
      181 ccagcgcgga tgaactgacg gccgagcagg cggcggcgaa gaccaccgcg gtcaaagcgt
      241 tcacccgcca gaggtcgtcg cgcaaaccgt ttccggctca tctgccccgt gagcgcgtcg
      301 tcgttcccgc cccgagcgcg tgcgcgtgct gtggatcgac caaactcgcc aagctgggcg
      361 agaccatcac ggagaccctg gaaagcattc cgcgccagtg gaaggtaatc cagacggtgc
      421 gggagaaatt cacctgccgg gaatgcgaga agatcacgca gccaccggca ccgtttcata
      481 cgatcccgcg gggctgggct gggccgagcc tcttggccat gatcctgttc gagaagtttg
      541 gccagcacca accgctcaat cggcagtgtg aacgcttcgc ccgtgagggt gtcgaagtga
      601 gcctgtcgac cctggccgac caggtcggga cctgcgccgg cgtgttgaag ccgctctata
      661 gcctgatcga aacccatgtg ctggcggccg agcgcctgca tggcgatgac accacggtgt
      721 cggtcctggc taagggcaaa acaatcaccg gccgattgtg gacctatgtc cgcgatgacc
      781 gcccgttcgg tggcccggca ccgccggcgg ccgtgttcta ttactcccgc gaccgcaccg
      841 gcgagcaccc ccagcgccat ctgcacactt atgccggcat cttccaggcc gacgcgtatg
      901 gtggatacaa caagctttat gaggagggac gccagcctag cctggttctg gaagcggcgt
      961 gttgggttca tgcccgccgc aggttcttcg tcctggccga cttggctgcc agcgcccggc
     1021 gcaaagcgca gggcaagacg ccggccgtca tctcgcccat cgctttggag gcggtctgtc
     1081 gcatcgacgc cctgtttgac atcgaacgcc agatcaatgg ccaggacgcc ggtcagcgca
     1141 gggccgtgcg ccaggaattg agcgcaccgc tggtcgccga gctggaagcc tggatgcgtc
     1201 aggaacgcgc caggttgtcg cgccacaacg acgtcgccca ggcgatggac tacatgctca
     1261 agcgctgggc ggcgtttgca cggttccttg atgatgggcg catctgcctc tccaacaacg
     1321 ccgccgagcg cgcggtgcgc ggaattgctc tcggacgtcg ggcatggtta ttcgccggct
     1381 ccgatcgcgg aggcgagcgg gcggcgttca tgtacagcct gatcgtcacc gccaagatga
     1441 acaacatcga tccgcaggct tggctcgccg acgtgctgtg ccacattgcc gatcacccgg
     1501 ttcacaggct cgacgaactc ctgccttgga attggaagcg ccaatctgcc aagctcgcgg
     1561 catgaaaaac aacgacattg ccgaagcggc aaggatgcgg caggggtaga agtgcaaaga
     1621 aggcgatcaa cgggcatatc agaggtagaa aaggcggccg tcaccgtcgc ctgtcagagg
     1681 tttatcgacg aagtcctaaa gccgcgattt ctgcccgtca tccaccgaac cgaattcaac
     1741 taccccatcg acattcacgg caaatggcat ggcgcacgat atcgcttcat ccagcgctac
     1801 cgttccggct tccccgagac gatgaatcag gagttcgata gccccttcgt gcggttggac
     1861 tgggtcgcc
//
LOCUS       DASWGM010000274         5087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_26049,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000274 DASWGM010000000
VERSION     DASWGM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26049"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VGA15_08000"
     CDS             complement(<1..1026)
                     /locus_tag="VGA15_08000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587672.1"
                     /translation="MSKTEDAFDVPAFINSRPIGAVQYGIVVLCGLVMFLDGLDTQAI
                     SYMAPMISKEWGLSREVLGPIFSSALTGLMFGYLVLSPLSDRFGHRRLILFSTVTFGL
                     LTLVTLVATNVTELMILRFLTGIALGAAIPSTVALTTEYMPKRLRATFVLAIYCGFSL
                     GFVAAGGLAASMIPLYGWRSMLWIGAATPLALAVLVYVFLPESLDFLVRTQAKPHGIW
                     RIVRRLDRALPSEGPRQFVTEVEAKRSAVGSLFQSGRTLGTLVLWLVFGLNLAEFYAL
                     QSWLPTILAGHGFSPNAVALATSMTTIGGIVAAFAIGPAMDKFGAYRSLAVVYFAGVV
                     FVALMGPA"
     gene            complement(1073..2083)
                     /locus_tag="VGA15_08005"
     CDS             complement(1073..2083)
                     /locus_tag="VGA15_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9587673.1"
                     /translation="MTFTVSVDASDIEFPCETGETVLDAAERAGYSLPYSCRKGVCAT
                     CEGDLCRGEVAVGSKHFAGPSDDVLLCQARPRSDVLIHPRRIQRRDPDARKTVTAKVY
                     RIHRPVDDVAILQLRFPAGIRAKFLAGQYLRIKMPDGDTRNFSMANPPHESDGVQLHI
                     RHVPGGRFSEGVLAELRPGDTLDVELPYGDFHLRPDSDKPVVCLATGTGFAPLKSIVE
                     DLIKRGNKRPVHLYWGGRRQRDLYLADLPRKWAARAGWFKFIPVLSEPDLDWNGAVGL
                     VHHAVLRDLTDLSGWEVYACGNPLMIRHARRDFRTTAGLSDGDFFADPFVPSGNDPAV
                     PG"
     gene            complement(2080..3075)
                     /locus_tag="VGA15_08010"
     CDS             complement(2080..3075)
                     /locus_tag="VGA15_08010"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX9587674.1"
                     /translation="MTSPAIALTIGDPNGIGPEIAVKAAAKLAREGGPAVVLIGDEFV
                     VRSYAESCAKDFSIQKFDGSPARAGALQFLAVDALSREDFRPGTPLAAGGRATVDYVA
                     AAMRFVSEGHAAAIVACPHSETNVNAAGIPFSGYPGLLARLMKVPEDEVFLMLVGGGL
                     RIVHVTLHERLHAALARITPDLVERATRTAEAALQRLQIKRPRIGLFGINPHAGEGGL
                     FGDDDDRITVPAARRLRESGLDVEGPLGADLMLGKQGFDAFVAMYHDQGHIPVKLLAG
                     RNSAALSIGTGLLFSSVGHGSAFDIAGRGVAESDAVLRSIQLVCGASRLDDAATA"
     gene            complement(3072..3542)
                     /locus_tag="VGA15_08015"
     CDS             complement(3072..3542)
                     /locus_tag="VGA15_08015"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX9587675.1"
                     /translation="MIDSVYQMIARAQAEYARCIDDDRLEEWPNHFHGQCIYRVTTAA
                     NHREGLEAGLIFANSRGMLADRVSALREANIYERQAYRHILGQPSILSEQNGEVRSET
                     PFLVARILQDGDTDIFATGRYLDLYKVEDGQVKLNERTVICDSSRIDTLLALPL"
     gene            complement(3559..4812)
                     /locus_tag="VGA15_08020"
     CDS             complement(3559..4812)
                     /locus_tag="VGA15_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX9587676.1"
                     /translation="MAQRQEIPSGGQAWPRDGLTRVPYRVFQTEEAYKSEQKLIFQGP
                     SWHYLALEVEVPEPGDFRTTKVGDTPVIVTRDADGEIYAFENRCAHRGALICLDNHGK
                     GKKAFSCVYHAWTYDRQGNLKGVAFKDGVKGKGGMPDSFRMEDHGPRKLRIATLHGLI
                     FGSFSEQVPPIEEYLGEEISSRIERVLGGRIPVVIGRVTQVLPNNWKLYMENVKDSYH
                     ASILHLFFTTFELNKLSQKGAIIVDESGGHHVSYSAIDREAQKDAEYAKQQLRSESQY
                     KLADPSLLQGFDEYHDGVTLQILSVFPTFVLQQIQNAIAVRQIVPRAIDKTDLNWTYI
                     GFADDTPEQRLTRVKQGNLVGPAGYISMEDGCVGGFVQRGIAGASDQEAVLEMGGGEA
                     RSSESRVTEASVRGFWKAYRSNMGV"
BASE COUNT         1036 a         1608 c         1514 g          929 t
ORIGIN      
        1 ggctggcccc atcagggcaa caaacaccac accggcgaaa taaaccaccg caagcgagcg
       61 gtaagcgcca aacttgtcca ttgcgggtcc gatcgcaaaa gccgcgacga ttccaccgat
      121 cgttgtcatc gaggtggcga gtgcgaccgc gttcggcgaa aagccatgcc ctgccaaaat
      181 ggtcggcaac cagctttgaa gcgcgtagaa ttcggcgagg ttcagaccga ataccagcca
      241 gagtacgagc gtcccgagcg tcctgcccga ctggaacaga ctgccgaccg cgcttctttt
      301 tgcttccact tcggtcacga attgccgcgg gccttcgctc ggcagcgccc ggtccagcct
      361 gcggacaatc cgccagatgc cgtgcggttt tgcttgggtg cggaccagaa aatcgagcga
      421 ttcgggcagg aagacataaa ccaacaccgc aagcgcgagc ggagtggccg ctccgatcca
      481 aagcatcgaa cgccagccat aaagcggaat catcgatgca gctaatccgc ccgcagcaac
      541 gaatcccagt gagaagccgc aataaatagc gagtacgaac gtcgccctca ggcgcttggg
      601 catgtattcg gtcgtcagag cgaccgtact cggtatcgca gcccccaaag cgatgcccgt
      661 gagaaaccgc agaatcatca gctcggtcac gttggtggcg acgagcgtca ccagcgtcag
      721 caacccgaac gtcactgtac tgaagaggat gagccgccga tgcccgaacc tgtccgacag
      781 aggcgacagc accaaatagc cgaacatcag acccgtgagc gcagaggaaa agatcggtcc
      841 cagcacttcc cgggagagtc cccactcctt tgagatcatg ggcgccatat agctgatggc
      901 ttgggtatcc aacccgtcca ggaacatcac cagcccgcaa agcacaacga tgccgtactg
      961 cactgccccg atcgggcggg aattgataaa ggcaggaacg tcgaacgcgt cctcggtctt
     1021 gctcacggtt ttttcctcga attatttgct gttgttttct ggggcgagcg gttcaaccgg
     1081 ggaccgccgg atcgtttccc gacggcacga agggatctgc gaagaagtcg ccgtcggaga
     1141 gaccagcggt cgtccggaaa tcgcgtcgcg catggcggat catcagtggg ttgccgcagg
     1201 catagacctc ccaccccgac aaatcggtca gatccctcag cacggcgtga tggaccaggc
     1261 ccacagctcc gttccaatcc agatcgggct ccgacaagac cgggatgaac ttgaaccagc
     1321 ccgcgcgcgc ggcccacttc cgcggcagat cggccaggta cagatcgcgc tgacggcggc
     1381 caccccaata caggtgcacc ggacgtttgt tgccacgctt gatcagatct tcgacgatcg
     1441 acttcaacgg cgcgaagccg gttccggtcg caaggcaaac aaccggcttg tccgaatccg
     1501 ggcgcagatg gaaatctccg tacggcaatt cgacgtcgag cgtgtcgcct gggcggagtt
     1561 cggcaagcac cccctccgag aaacgtcccc ccggcacgtg ccggatatgc aactgcacgc
     1621 cgtcgctctc gtgcggcggg ttcgccatcg aaaaattgcg ggtgtcgccg tcaggcattt
     1681 tgatgcgcag atattggccg gccaggaatt ttgcgcgaat gcccgccgga aaacgcaact
     1741 gcaggatggc cacatcgtcg accggccgat ggatgcgata gaccttggcg gtcaccgtct
     1801 tgcgcgcgtc gggatcgcgg cgctgaattc tccgggggtg aataagaaca tcggaccggg
     1861 gcctagcctg gcagagcaga acgtcatcgc tgggaccagc gaaatgcttc gaacccacgg
     1921 cgacttcgcc ccggcaaagg tcgccctcgc acgtcgcgca gacgcctttc ctgcatgaat
     1981 agggcagcga gtagccggcg cgctcggccg catcgagaac cgtttcgccg gtttcgcagg
     2041 ggaattcgat atcgctggcg tcaacagaaa ccgtgaacgt catgctgtcg ccgcatcgtc
     2101 gaggcgagac gctccgcata cgagctggat cgatcgcaac accgcgtcag actcggcgac
     2161 gccgcggccc gcgatgtcga aggcgctgcc gtggccgaca cttgaaaaca aaagccctgt
     2221 cccgatggat agcgctgccg aattgcggcc ggccaggagt tttaccggaa tatgtccctg
     2281 atcgtggtac atcgcgacaa aggcatcgaa accctgcttg ccgagcatga gatcggcgcc
     2341 gagcggtccc tcgacatcga gaccggattc ccgcaaccgc ctggccgccg gaacggtgat
     2401 cctgtcatca tcgtcgccga acagcccccc ttccccggcg tgaggattga taccgaacag
     2461 tccgatgcgc ggccgcttga tctgcagcct ttgcagagcg gcctcggcgg ttcgcgtcgc
     2521 gcgctccacc aggtcgggcg tgatccgggc cagcgcggca tgtaaccgct catgcagcgt
     2581 gacatgaacg atccggagac cgccgccgac cagcatcagg aagacctcat cctccgggac
     2641 cttcatcagc cgcgcgagaa gacccggata gccggaaaac gggatgccgg ccgcgttgac
     2701 gttggtctcg gaatgcggac aggccacgat cgcggcggcg tgaccctccg acacaaatcg
     2761 catcgccgcc gcaacatagt cgacggtcgc ccgcccacct gcggccaacg gcgttcccgg
     2821 gcggaagtcc tcccgggaca aggcatcgac ggccaggaat tgcaaggcgc cagcgcgcgc
     2881 tggcgatccg tcgaacttct gaatggaaaa atccttcgcg caggactccg cgtaggatcg
     2941 gacgacaaac tcgtcgccga tcagaacgac cgccggacca ccctcacgcg caagtttggc
     3001 ggccgccttt accgcgatct ccggcccgat gccgttcgga tcgccaatgg tcaacgcaat
     3061 ggcgggagac gtcacaacgg cagcgccagt agcgtatcga tgcgcgagct gtcgcagatg
     3121 acggtccgct cgttcagctt cacctgaccg tcctcgacct tgtaaaggtc gagatatcgc
     3181 ccggttgcaa agatgtccgt atcgccgtcc tgcaagattc gcgcgaccag gaacggcgtt
     3241 tcgctgcgca cttcgccatt ctgttcggaa agtatcgagg gctgccccag gatatgacgg
     3301 tacgcctgac gctcgtaaat atttgcttcg cgcagcgccg aaacccgatc ggccagcatg
     3361 ccgcgcgaat ttgcgaagat aagtcctgcc tcaaggcctt cgcggtggtt ggccgccgtc
     3421 gtgaccctgt agatacattg tccgtggaaa tgattgggcc actcctccag ccggtcgtcg
     3481 tcgatgcatc gcgcgtattc ggcctgggcg cgggcgatca tttgatacac actatcgatc
     3541 atgtcttccg tcccgctttc acacgcccat attgctgcga tacgccttcc agaatccgcg
     3601 aacggaagct tccgtaaccc ggctctcgct gctgcgggcc tcaccgccgc ccatttcgag
     3661 aaccgcctcc tgatccgatg cgccggcgat gccgcgctga acgaaaccac cgacgcaacc
     3721 atcctccatg gagatgtagc ccgcgggccc gacgagattg ccctgcttga cgcgggtcaa
     3781 tcgctgttcg ggggtgtcgt cggcgaatcc gatataggtc cagttgagat cagtcttgtc
     3841 gatggcgcgc ggcacgatct gccggaccgc gatagcgttt tgaatttgct gcagaacgaa
     3901 ggtgggaaag accgataata tctgcagcgt gacgccgtcg tgatattcgt cgaacccctg
     3961 cagaagcgag gggtcggcga gcttgtattg gctttccgag cgcagctgct gcttggcgta
     4021 ttccgcatcc ttctgggctt cgcgatcgat ggcggaataa ctgacgtgat ggccgccgct
     4081 ctcatcgacg ataatggcgc ccttctgcga cagcttgttc agctcgaacg tcgtgaagaa
     4141 caggtgcaga atgctggcat ggtaggagtc cttgacgttc tccatgtaga gcttccagtt
     4201 gttgggcagc acctgcgtga cgcggccgat gacaaccggg attcgaccac ccaacacgcg
     4261 ttcgatgcgg gatgaaattt cctccccgag atattcttcg atcgggggaa cctgttccga
     4321 gaacgatccg aagataagac catgcaacgt ggcgatccgc agtttgcgcg ggccatggtc
     4381 ttccatccgg aaactatcgg gcatgccgcc cttgcctttg acgccgtcct tgaatgccac
     4441 gcccttcagg ttgccctgcc ggtcgtaggt ccaggcgtga tagacgcagc tgaaggcctt
     4501 tttgccctta ccgtggttgt ccagacagat caatgcgccg cgatgcgcgc agcggttctc
     4561 gaaggcgtag atctcgccat cggcgtcgcg ggtgacgatc accggcgtgt cgccgacctt
     4621 cgtggtgcgg aaatcgccgg gctccggaac ctccacttca agcgcgaggt agtgccagct
     4681 cggtccctgg aagatgagct tttgctccga cttgtaggcc tcttcggtct ggaacacccg
     4741 gtagggcacg cgtgtcagcc cgtcccgcgg ccacgcttgc ccaccgctcg ggatctcttg
     4801 acgctgcgcc atgatcaaac gcctctgctg gccggattgg ttgactttcg aagcccgaag
     4861 tgttcggtta tcgaacatat agctctaatc cgaacagatt tgaccgtttc caaacacctg
     4921 tcaagggtaa cctgatgccg caaaagaccg aaaggcccgc gcgcaaggcc gaaggaatgg
     4981 gcggcctcgc caaggggctt gcgattgtcg aggcgttttc tgctcatccc gtcatgagcg
     5041 tcgccgacgc tgcgcgagcg gcgggcgcga cgcgcgcatc cgctcgc
//
LOCUS       DASWGM010000275         2928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1689171,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000275 DASWGM010000000
VERSION     DASWGM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689171"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGA15_08025"
     CDS             <1..370
                     /locus_tag="VGA15_08025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9587677.1"
                     /translation="IEWLNDSLPELALVRTRGESFDLVMLTAVWMHLDARQRQRAMPN
                     LAALAREGGTVIMKIRHGPVPPGRRMFEITPEETIGLARMQGLHPVVHLRTESSQERN
                     RAAGVTWTNLAFVKAKVPAA"
     gene            complement(367..1047)
                     /locus_tag="VGA15_08030"
     CDS             complement(367..1047)
                     /locus_tag="VGA15_08030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEX9587678.1"
                     /translation="MPAIPSNKPYRVGRSRTGLGLFATKPIKKGAKIVRYFGPLLDSR
                     KKKDDAIENKYLFELNNRWTIDGSVRKNIARYINHACKPNAESDVKPRKRKVVIRAIK
                     NIEPGDEINYDYGTDYFKAYLKPIGCKCDACEKKRKKKRAEARAEKLRLKAKAEKKAL
                     RKAEKQAAKAREKLEQNSKQKLNGKSNGELLNGHGGKSAAGKMIAAKSGIFAKPGGKP
                     DRETGASA"
     gene            1243..1854
                     /locus_tag="VGA15_08035"
     CDS             1243..1854
                     /locus_tag="VGA15_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587679.1"
                     /translation="MQKGLGIALAALCYADSGLISPAEAQTFGSTYTSTAPRDCRVSS
                     AGNGVDDSTTRVCPGKAGLVVLVSEDDLRETVSVGRNRAAATREPAAEVWFGPFNSTT
                     TTVEWRAVDGKPFAIIQRWHLADNSNADKDGRPVAKPMLAVTRLPPGAVCHVAYVDVK
                     ANPNANELARKAADETARDFKCGSDAVKVIGESGRAVELATGR"
     gene            complement(1856..2587)
                     /locus_tag="VGA15_08040"
     CDS             complement(1856..2587)
                     /locus_tag="VGA15_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HEX9587680.1"
                     /translation="MAKKPSNARTRTAPTKAASPARGRRKPGAPRSGGASEEAPYHHG
                     ALRDALLEAAERVLERDGLAGLTLRAVAREAGVSHAAPTHHFGDLTGLVSELAAIGFR
                     QFNTAMAAAGSAAGPALMEKALARAKAYVAYAQAHPGMYGLMFRTERLDMKRPALHEA
                     AEASFAGLAGAIGASRQEQISETALSLDQAAAIARAWSLVHGFTMLLLDDRLCDILRR
                     LPEGTDAETLLDAMLKSTIGRPPGL"
     gene            2698..>2928
                     /locus_tag="VGA15_08045"
     CDS             2698..>2928
                     /locus_tag="VGA15_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX9587681.1"
                     /translation="MQQEAATRAARRNLAPIPMECDAPFLKVQGELPRELNGTLYRNG
                     PNPQFDAPGAHWFVGDGMLHAFHLENGRASYRN"
BASE COUNT          510 a          925 c          929 g          564 t
ORIGIN      
        1 gatcgaatgg ctcaacgaca gcctgccgga gctcgccctg gtgcggacgc gcggcgagag
       61 ttttgacctc gtcatgctca ccgcggtctg gatgcatctc gatgcgcggc agcgccaacg
      121 cgccatgccc aaccttgccg cgctggcgcg cgaaggcggc actgtcatca tgaagatccg
      181 gcacgggccg gttcctccgg gacggcggat gttcgaaatc acgccagagg agacgatcgg
      241 gcttgcgcgg atgcaaggct tgcatccggt tgtccatctg cgcacggaat ccagtcagga
      301 acgaaatcgc gccgctggcg tcacctggac aaatctggcg ttcgtgaagg cgaaggtgcc
      361 cgcagcctaa gccgaagcgc cggtttcccg atctggcttg cccccgggct tggcgaatat
      421 cccggatttc gcggcaatca ttttgcccgc ggcggatttc ccgccgtgcc cgttcagaag
      481 ctcgccattc gacttgccgt tcagcttttg cttggaattc tgctcgagct tctccctggc
      541 cttcgccgcc tgcttctcgg ctttcctgag cgctttcttc tcggccttgg ccttcagccg
      601 cagtttttcg gccctcgcct cggcgcgctt tttcttgcgc ttcttctcgc aagcgtcgca
      661 tttgcagccg atcggcttca gatacgcttt aaaatagtcg gtgccgtaat cgtagttgat
      721 ctcgtcgccg ggttcgatgt tcttgatggc gcggatcacg accttgcgct tgcgcggctt
      781 gacgtcggac tccgcatttg gcttgcaggc gtgattgatg tagcgcgcga tgttcttgcg
      841 caccgagccg tcgatggtcc agcggttgtt gagttcgaac agatatttgt tctcgatcgc
      901 gtcgtctttc ttcttccggg aatccagcaa cgggccaaaa tagcggacga ttttggcgcc
      961 cttcttgatc ggtttggtgg cgaacaggcc aagcccggtg cgggaacggc cgacgcgata
     1021 gggtttattc gagggaatgg caggcatgat gattgaggaa ctgacacgct agttgagggc
     1081 gacgggtcgc ggagcgctgt tctaaaacga ttccagtgga atgtcaggcc tttcgcgcat
     1141 ccggcttgaa ccttccccag attgcgtacg tcgaaactat cagcggttcc gcaaaaaggc
     1201 ggcaggatga agcacgcacc caaccacccg atctcctcag ccatgcagaa ggggcttgga
     1261 atagcgctgg cggctctgtg ctatgccgat tccggcctga tttcgccagc agaagctcag
     1321 acctttggca gcacctacac ctctaccgcg cccagggatt gccgcgtgtc gagcgccggc
     1381 aacggcgtcg atgactcgac gacaagggta tgcccgggca aggccgggct cgtggtgctc
     1441 gtgagcgagg atgatctgcg cgaaaccgtc tcggtcggcc gcaaccgcgc tgccgcgacc
     1501 cgggaaccgg ccgccgaggt ctggttcggt ccattcaatt cgaccaccac gaccgtcgaa
     1561 tggcgcgcag tggacggcaa gccgtttgcg atcattcagc gctggcacct cgccgacaac
     1621 agcaacgcgg acaaggacgg tcggccggtc gccaagccga tgctggcggt gacgcggctg
     1681 ccacccggcg cggtgtgcca tgtggcctat gtcgacgtga aggccaaccc caatgccaac
     1741 gaactggcgc gcaaggccgc cgacgaaacc gcgcgcgatt tcaaatgcgg aagcgacgca
     1801 gtcaaagtga tcggtgaaag cggccgcgcg gtcgaactgg caacggggcg ctgattcaaa
     1861 gccccggcgg ccggccgatc gtcgacttga gcatggcatc cagcagggtt tcggcgtcgg
     1921 tcccctcggg cagccgccgc agaatatcgc agagccggtc atcgagcagc agcatggtaa
     1981 aaccgtggac cagcgaccag gcccgcgcga tcgccgcggc ctgatcgagc gacagcgccg
     2041 tttcggagat ttgctcctga cggctggcgc cgatcgcgcc ggcgagcccc gcgaacgagg
     2101 cctcggcggc ctcgtgcagc gccggccgct tcatatcgag gcgctcggtg cggaacatca
     2161 gcccgtacat gccgggatgc gcctgggcat aggcgacata ggccttggcc cgggccagcg
     2221 ccttttccat cagcgcaggg cccgcagctg aacctgcggc cgccatcgcc gtgttgaatt
     2281 gccggaaccc gatcgcggcg agttcgctga caaggccggt gagatcgcca aagtgatgtg
     2341 tcggcgcagc atgcgaaacc ccggcctcgc gcgccaccgc gcgcagggtc agtcccgcca
     2401 gcccatcgcg ttcgagcacc cgctcggccg cctccagaag tgcgtcgcgc aacgcgccgt
     2461 gatggtacgg agcctcttcg ctcgcaccgc cgctccgcgg cgcgcctggt ttccgcctcc
     2521 cgcgcgccgg cgacgcggcc ttggttggag cggtgcgggt tcgggcattg ctgggttttt
     2581 tcgccatttg cgccttatag gctccgattt ttacactgta aagattttgc ttgacggctg
     2641 cggtcacgcc gcatagccta tcttgacgat gtaaagatag ggtaaaggga gcaatcgatg
     2701 cagcaggaag cagcgacaag ggcggcgcgg cgcaatctgg cgccgatacc gatggaatgc
     2761 gacgcgccgt ttcttaaagt gcagggtgag ttgccacgcg aactcaacgg cacgctctat
     2821 cgcaatggcc ccaatccgca gttcgacgcg ccgggcgcgc actggtttgt cggcgacggc
     2881 atgctgcacg ctttccatct cgagaacggc cgcgccagtt atcgcaac
//
LOCUS       DASWGM010000276         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1689259,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000276 DASWGM010000000
VERSION     DASWGM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689259"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGA15_08050"
     CDS             complement(<1..513)
                     /locus_tag="VGA15_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9587682.1"
                     /translation="MTKPAPSPSVQKLPVSLAANPTLSSWVRFSPEGHVKVSPGKVEI
                     GQGIVTALAQIAADELDLDIGRVQMVRASTAASPNEGVTSGSLSVQQSGRAIRHVCAE
                     VRQIFLGLASDRLGVAIDALAIEDGTISGPGNIRTSYWELADEVSLERDAMPGAVPTP
                     STQRTLAGHSI"
     gene            complement(510..980)
                     /locus_tag="VGA15_08055"
     CDS             complement(510..980)
                     /locus_tag="VGA15_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9587683.1"
                     /translation="MPSIRFRLNGVETDVDADPDSSLLSILRGPMRMTGPHFGCGANE
                     CGACHVIVGDHAVASCDTPLWSVADKDITTIEGLGSAEKPHPLQRAFIAEQALQCGYC
                     VSGILMSAAALLMRNPAPSSDDVRAALDRNLCRCGSHNRMVRAVLRAAAEIATQ"
     gene            complement(999..1991)
                     /locus_tag="VGA15_08060"
     CDS             complement(999..1991)
                     /locus_tag="VGA15_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9587684.1"
                     /translation="MTAPRFALAFAATLLATPILVATPILVATQCLAQDYPTRPVRVI
                     VPFGAGGPADVTARLIGNVLQENFGQPFVVENRTGAGGVIGTLEAAKSPPDGYTLLMM
                     SNTQTANESLVPQRKYELMRDLAPIAPVNYSDLVIVVHPQVAAKTLAEFIALAKSQPG
                     KLNYASSGQGTPYHMAGELFKTMAGIDVVHVPYRNSGEARSGVIGGQVQMMIDAVPAM
                     VPNIGENQVRALATTGKTRSGVLPNVPTASEAGVPGYEATIWLGLMAPAGTPKPIIDK
                     LNAAVNAAIKRPDIVKLWTEQGAVPMSMTPEEFDKYLRGDIVKWAEVVKKFDKP"
     gene            2167..>3212
                     /locus_tag="VGA15_08065"
     CDS             2167..>3212
                     /locus_tag="VGA15_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9587685.1"
                     /translation="MNNPNISRRTLLKASGALLAGAAFSTRVIAAAPPPEPITPALIE
                     AAKKEGQVIYYTSTDLPVAEKLAKAFEAKFSGIAVRVERTGAERVFQRIGQEYSSNIH
                     AVDVVNSSDAAHFIVWKRDGILAPYVPEDVAKFYPPEHKDTDGQFASWRVWLSIIAYN
                     TNLVKPEEAPKSFADLLDARWKGKIVKAHPGYSGTIMTATYQMQRDLGWAWFEQLAKQ
                     NIMQVQSSADPPKKLDLGERAVMADGNEYNIFQMKEAGRPVAPVYASEGSPLIIGPNG
                     IFKASPNPNAAKLFQSFCFSRDAQQLIIDVGGLRSAHPQTQEKAGRTPFKDIKTMKDD
                     AAAVEKEGDAIKAR"
BASE COUNT          635 a          974 c         1003 g          600 t
ORIGIN      
        1 aatggaatgc cctgcaagcg tgcgctgcgt cgatggtgtt ggcacagcgc ccggcatcgc
       61 gtcgcgctcc agcgagactt catcggccaa ttcccagtag ctggtcctga tattgccggg
      121 tccggagatg gtgccgtcct cgatggcgag cgcatcaatc gcgacgccga gccgatccga
      181 cgccagccca agaaagattt gccggacctc ggcgcagacg tgcctgatgg cgcgtcccga
      241 ctgctgaacg gaaagactgc ccgacgtcac gccctcattg ggactggcag ccgttgacgc
      301 gcggaccatc tgcacccggc cgatatcgag atcgagctcg tcggcggcga tctgggccag
      361 tgccgtcacg atgccttgcc cgatctcgac ctttcccgga gaaactttca cgtggccctc
      421 cggcgagaac cggacccatg acgacagcgt cgggtttgcg gcaaggctga ccggcaattt
      481 ttgcacggag ggcgagggcg cgggctttgt cattgggttg cgatctcggc agcggcgcgc
      541 aacaccgccc gcaccatgcg gttatgtgag ccgcaacggc aaagattgcg gtccagcgca
      601 gccctcacgt cgtcgctgga aggcgcagga tttcgcatca aaagtgccgc ggcgctcatc
      661 aagatgccgg acacgcaata gccgcattgc agggcctgtt cggcgatgaa cgcgcgctgc
      721 agcggatgcg gcttttccgc gcttccaagc ccctcgatgg tggtgatgtc cttatccgcc
      781 acggaccaca gcggcgtgtc gcatgacgct accgcgtggt cgccgacgat gacgtggcag
      841 gcgccgcatt cattggcgcc gcagccgaaa tgcgggccgg tcatgcgcat tggaccacgc
      901 aggatactga gcaacgagga atcgggatcg gcgtcgacat cagtctcgac gccattgagg
      961 cggaatcgaa tactcggcat tcaggctgcg accttgattt acggcttgtc gaacttcttg
     1021 acgacctcgg cccattttac aatatcgccg cgcagatatt tgtcgaattc ttccggggtc
     1081 atcgacatcg gaaccgcgcc ctgttcggtc cagagcttga cgatgtcggg ccgtttgatc
     1141 gccgcgttga ccgcggcatt gagcttgtcg atgatcggct ttggcgttcc cgcgggcgcc
     1201 atcaatccga gccagatggt cgcctcgtat ccggggacgc cggcctcgga ggccgtcggc
     1261 acgttgggca gcacgcccga acgggtcttg ccggtggtgg cgagtgcccg cacctggttt
     1321 tccccgatgt tggggaccat cgccggaacc gcgtcgatca tcatctgcac ctgtccgccg
     1381 atgacgccgc tgcgcgcctc gccgctgttg cggtaaggga catgcacgac atcgatgccg
     1441 gccatcgttt tgaacagttc gccggccatg tgatagggcg tgccctggcc ggatgaggcg
     1501 tagttcagct ttcccggctg cgatttggcg agcgcaatga attcggccag cgtcttggcc
     1561 gcgacctgtg ggtgcacgac gatgacgagg tccgagtaat tgaccggtgc gatgggcgca
     1621 agatcgcgca tcaattcata tttccgctgc ggcaccagcg attcgttcgc ggtctgggta
     1681 ttcgacatca tcaatagcgt atagccgtca ggcggcgact tggcggcctc cagcgtcccg
     1741 atcacaccgc cggcgccggt gcggttctcg accacgaagg gctgaccgaa attctcctgc
     1801 aatacgttgc cgatcaggcg ggccgtcacg tcggccgggc ccccggcgcc aaagggcacg
     1861 ataaccctga ccggccgcgt cggataatcc tgcgccagac attgcgtcgc aacaaggatt
     1921 ggcgtcgcaa caaggattgg cgttgcaaga agagttgccg cgaacgcaag cgcaaacctt
     1981 ggggccgtca tttcaatctc ccgaatccag gtcttgttct acgttggact gtggttggcc
     2041 aatgcagcta tcaagtctag ctgcaacggc aatgagctgt cgacgataga ctaggtgtgc
     2101 gccgtaggaa taaaagataa cctcagctat accgaaccat gccagaacaa gcggatggaa
     2161 gcgccaatga ataatcccaa tatatcgcgc cgcactttgt tgaaggcgtc gggcgcattg
     2221 cttgcgggtg ctgcgttctc gacccgcgtc atcgcggcag cgccgccgcc tgagccaatc
     2281 acgccagcgc tgatcgaggc ggcgaagaag gaaggccagg tcatctacta cacctcgact
     2341 gatctgccgg tcgcggagaa actggcaaag gcgttcgagg cgaaattttc cgggatagcg
     2401 gtgcgcgtcg agcgcaccgg ggccgagcgt gtgttccagc gcatcggcca ggaatattca
     2461 agcaacattc acgcggtcga cgtggtgaat tcgtcggacg ctgcgcattt catcgtctgg
     2521 aaacgcgacg gcattctggc gccctacgtg cccgaagatg tcgcgaaatt ctatccgcct
     2581 gagcacaagg acaccgacgg ccagtttgcg agctggcggg tctggctcag catcattgcc
     2641 tacaacacta atctggtgaa gccggaggag gcgccgaaga gttttgccga tctgctcgat
     2701 gccagatgga agggcaagat cgtcaaggcg catcccggct atagcggcac catcatgacc
     2761 gcgacctatc agatgcagcg cgatctcggc tgggcctggt tcgagcaact tgccaaacag
     2821 aacatcatgc aggtgcaatc ctcagccgat ccgccgaaga agctagatct cggcgagcgg
     2881 gcggtcatgg ctgacggcaa cgagtacaac attttccaga tgaaagaggc cggccggccg
     2941 gtcgcgccgg tctatgccag cgaaggttcg ccgctgatca tcggaccgaa cggcatcttc
     3001 aaggcctcgc ccaatccgaa tgcggcaaaa ctgtttcagt cgttttgctt cagccgggac
     3061 gcccagcagc tcatcatcga tgtcggcggc ctgcgctcgg cgcatccgca gacccaggag
     3121 aaggcggggc gcacgccgtt caaagacatc aagaccatga aggatgacgc cgccgcggtg
     3181 gaaaaagagg gcgacgcgat caaggcgcgc ta
//
LOCUS       DASWGM010000277         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2382975,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000277 DASWGM010000000
VERSION     DASWGM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382975"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGA15_08070"
     CDS             <1..282
                     /locus_tag="VGA15_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587686.1"
                     /translation="VGGIAGAMIGAAIGVLSPLLSLPLTTKGLIVTVIGGLGSIPGAI
                     IAGLLVGGFENLFQYFRGVTERDLYVMLLLFAFLVFRPGGIFSPAAGRD"
     gene            292..1221
                     /locus_tag="VGA15_08075"
     CDS             292..1221
                     /locus_tag="VGA15_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9587687.1"
                     /translation="MDFYLGLAQIVGVHTLLGLSAYCVLLTGQVSLAQAGFFAIGAYV
                     AAMLTVLAGWHIAPAIAAASFVAGAIACAIGFPALRVKGLMLVVATLAFGEAVRLFFF
                     NFDYQVARGEAKIGPAGGEGFRQIRFFPEHGWSTLDVLMFIWSFVALVMAALWWMDRS
                     RAGAVLRAVGEDEVAAQSAGINVTAVKVAAMTVGGFIAGLGGGIFAHYTAHIEHAQFG
                     VALATFAIAYPILGGLSSVFGTLIAVIFVQGFLIEGLRFLGDWRNLLFGGLILLTMNV
                     RPRGLIDAATIAAIKRMRIRNREPGNARTQGAE"
     gene            1196..>1852
                     /locus_tag="VGA15_08080"
     CDS             1196..>1852
                     /locus_tag="VGA15_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9587688.1"
                     /translation="MLELKGLSKHFGGVRAVDGLDLNVPQGEVFGLIGPNGSGKSTVV
                     NLVCGLFPVTAGQVLLRNEDITELASHMRVVRGVTRTFQNIRLFGQLSVWQNLWVAQN
                     AIEHRSEGFLRRWLGGPGRAREEIDRILEFSDLSGKRDELAGNLAFGEQRRLELARAI
                     VARPALLLLDEPAAGMNAEEIDQLDARIRKLKQDGLTVLLIEHHMELVMAVTDRIAVL
                     N"
BASE COUNT          274 a          552 c          607 g          419 t
ORIGIN      
        1 gtcggcggca tcgcgggcgc catgatcggg gccgcaatcg gcgtgctgtc gccgctgctg
       61 tcgctgccgc tgaccaccaa gggactgatc gtcaccgtga tcggcggatt aggctcgatt
      121 ccgggcgcga tcatcgccgg cctgcttgtc gggggtttcg agaacctctt tcagtatttt
      181 cgcggcgtca ctgagcgcga cctctatgtc atgcttctgc tgttcgcgtt tctggtattc
      241 cggccgggcg gcattttttc ccccgccgct gggcgggatt gaggagaccg catggatttc
      301 tatcttgggc tcgcccagat cgtcggtgtc cacacattgc tggggctttc tgcctattgt
      361 gttctgctga ccggacaggt cagtcttgcg caggctggct tctttgctat cggcgcttat
      421 gtcgcggcga tgcttacggt gcttgctggc tggcatatcg cccccgcgat tgccgccgct
      481 tcgttcgtcg ccggcgcaat cgcctgcgcg atcggcttcc ctgcgctgcg cgtcaaaggc
      541 ctgatgctgg tcgtcgcgac gcttgcgttc ggcgaggccg tgcggttgtt tttcttcaac
      601 ttcgactatc aggttgcgag gggcgaagcg aagattggcc cggctggcgg cgaaggcttc
      661 cgacagatca gatttttccc ggagcacggc tggtcgacgc tcgacgttct gatgttcatc
      721 tggtcgtttg tcgcgctcgt gatggcggca ttgtggtgga tggatcgctc gcgcgccggc
      781 gccgtgctgc gcgccgtggg tgaggatgaa gtcgccgcac agagcgccgg gatcaatgtg
      841 acagcggtca aggtggcggc gatgaccgtc ggcggcttca ttgccggtct cggcggcgga
      901 atcttcgccc actacaccgc ccatatcgag cacgcccagt tcggcgtcgc gctcgccacg
      961 tttgcgatcg cctatcctat cctcggcggt ctctcgagcg tgttcgggac tttgatcgcc
     1021 gtcatcttcg tccagggctt tctgatcgag gggctgcgct tccttggcga ctggcgcaac
     1081 ctgttgttcg ggggcctcat tctgctcact atgaatgtca ggccgcgtgg actcatcgat
     1141 gcggcgacga tcgccgctat caaacgcatg cggattcgca acagggagcc gggcaatgct
     1201 cgaactcaag gggctgagta aacatttcgg cggcgtgcgc gccgtcgacg gtctcgacct
     1261 caatgttccg cagggtgagg tgttcggcct catcgggccg aacggctccg gcaagtcgac
     1321 tgtcgtcaat ctcgtctgcg gcctgtttcc ggtcacggcc ggccaggttc tgttgcgcaa
     1381 cgaggacatc actgagcttg cctcgcatat gcgggtagtg cgcggggtta cccgcacctt
     1441 tcagaacatc agattgtttg gccaactcag cgtctggcag aatttgtggg tggcgcagaa
     1501 cgcgattgaa caccggagcg aaggctttct gcgccgctgg cttggcggtc cgggccgcgc
     1561 gcgcgaggaa attgaccgaa tcctggagtt ttccgatctg tcgggcaagc gcgacgaact
     1621 cgcgggtaat ctcgcgttcg gcgaacagcg ccgcctcgaa ctcgcccggg ctatcgttgc
     1681 aaggccggcc ctgctgctgc tcgacgagcc agccgcggga atgaacgccg aggagattga
     1741 ccagctcgac gcgcgcatcc gcaagctgaa gcaggatgga ctcacggtgc tgctgatcga
     1801 gcatcacatg gagctggtga tggcggtgac ggatcgcatt gcagttctca ac
//
LOCUS       DASWGM010000278         4643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2244346,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000278 DASWGM010000000
VERSION     DASWGM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244346"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..897)
                     /locus_tag="VGA15_08085"
     CDS             complement(223..897)
                     /locus_tag="VGA15_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9587689.1"
                     /translation="MSKAAEFAVILKMNPMFADLGTDELARISGLCHTQQLGLGEVLF
                     QKGDSGDALFGVRRGQIRIETGAADGSRLTLNFMGPGDLFGEVAVLDGQSRTADATAG
                     EPTELFVLRREDFLSHLEREPRVAIKIIQLLCQRIRWQSERMEESVLQPLPVRLARRL
                     CALATDYGSEVHISQEQLGVFVGAARESVNRQLQLWRKDGILDLQRGRILLLNMNRLT
                     AVARNE"
     gene            1141..3144
                     /locus_tag="VGA15_08090"
     CDS             1141..3144
                     /locus_tag="VGA15_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX9587690.1"
                     /translation="MRALKIAGAAVAAVIVIVAVLLILGIPSGFLTSAIQDRVERETG
                     YRLTIAGSTKIGLWPSLNVRVNDVTLEAPKDRDASNRVTVGSVQADMTLKSVWSGHPQ
                     ITELVITRPVLHHPLLRERERLPAPATSPAASSDGTDANALAIDRVTIVDGTMVMFNL
                     RDRVENRIEGINAEALIGGDRKIKVSGRARAGEQPLKFAITATAPAPPLQRQNIPVEL
                     TLDASGLLQAPLAAKAEVRLNGSIVMINGVTGTLGDGAFNGWASVDLSSKPLVKLDLD
                     FQRLDVATSRERATSASLGSQPWSNASIDLTGLNYVDAQVRVSAAELNIGEAHFAPVA
                     IDTAVAAGVLKTSFSNLGVYGGQASGQLVVDVTSGSPGYAFSSDLVGVRALPLLRSAA
                     DFDKLDGKMQAKMTLRSAGNSQHLVMSNLSGTVFVVFQDGAIRGLNVAAMIRSLTSGT
                     LAGWQEGKEQITDLTQLSASFRIDKGQATTADLNLVGPLVKMTGTGTIDLAAKALALR
                     VEPKLVLTTEGQGRTSDPVGLGIPVVIDGPWAEPRIYPDMAGILDNPDAAYAKLKEMG
                     KGLFGSSGGLGSILGGLGGSGGSGSSGSGSGSNGLSDQIGSTLGNLLQQGLGQRRSQN
                     RTLAAPASPTPDAPPASAQNDPAPEVSQDSQPMNDVLKQLFNR"
     gene            3483..>4643
                     /locus_tag="VGA15_08095"
     CDS             3483..>4643
                     /locus_tag="VGA15_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9587691.1"
                     /translation="MIGVKDKIWFLREGLFAKYVMSLVGLVVFVLAVNGATETWISYR
                     ATKTTLIDAMSEKAEATARRIEQSISELERQISWVTRASAKTYSGAVTLEDHHADYVQ
                     LLNQVPAVSQLTLLDQNGRGQLRLSRTTIAVGSKADLSRDVSLTETIARGGVSYAPAF
                     FQGSRPLMSIAVSHSGSDPSAAATVAEIDLGLLSDFLGDAQVGKTGFAYVIDPKGQVL
                     ASSARGPDVAKDLSKLPQVAALIRPNSQALASGTDVDRHAVLTASNAIPKLGWFVCFE
                     QPTAQALAPIRDQLVRIALLISLGLGVAILAGTVLARRMLVPITALRTGARRLGAGDF
                     SHRIDVHTKDELEELADQFNSMAGQLHETYSGLETKVEERTRDLAQSVNELKV"
BASE COUNT          865 a         1508 c         1459 g          811 t
ORIGIN      
        1 ccccgtcgca cggcgtacga cggcggcaac ccgttttgca tggcgaggct gcgtgttgag
       61 gattgttaaa aaatattaat gtcccaaatt taaaatattg gtggctcaat tgaggtcatg
      121 ttcgagcgcg ctgcgagctg aaagatgttc gcgcgccgca gttggcccac aattgcaagg
      181 cggagcgcag caaaatcagg gcaacgatgt ggtgcggctt tgctactcgt tacgcgccac
      241 tgcggtgagc cggttcatgt tcagcagcag aatgcggccg cgctgcaggt cgagaatgcc
      301 gtccttgcgc cagagctgca actgacggtt gacgctttcg cgcgcggcgc cgacgaagac
      361 cccgagctgc tcctgcgaga tgtgcacctc ggatccgtaa tcggtagcca gcgcgcagag
      421 ccggcgcgcc agacgcaccg gcagcggctg cagcaccgat tcctccatcc gctcgctctg
      481 ccagcgaatc cgctgacaca acagctggat gattttgatc gcgacgcgcg gctcgcgctc
      541 gagatggctg agaaaatcct cgcgccgcag cacgaacagt tcagtcggct cgccggcggt
      601 ggcgtccgcg gtgcggctct ggccgtcgag caccgcgacc tcgccgaaca gatcgcccgg
      661 gcccatgaaa ttcagggtca ggcggctgcc gtcggcggcg ccggtttcga tccggatctg
      721 gccgcggcga accccgaaca gcgcgtcgcc gctgtcgccc ttctggaaca gcacctcgcc
      781 cagcccaagt tgctgggtgt ggcaaaggcc ggatattcgc gctaattcgt cggtacctaa
      841 gtccgcgaac atcggattca tcttcagaat gaccgcaaat tcggccgctt tactcatggc
      901 aatgccttcc acgctagttc gaaaccgtca aaatgggctg ttatatctta tttcgtaaaa
      961 atcatctcgt ttcgaagtgt gtcataagtc acagaccttt gcgacaaccc tggattattt
     1021 ttggcggctt tgggccaatc cggttccagg atagacttgt ttcaggcgta gacttgggca
     1081 atccatcacg ccctgatatg gcgtggcgag gtcagacgcc gcccggtttg gaacatcgtc
     1141 atgagagcac tgaaaatcgc cggcgccgcc gtcgcggccg tcatcgtcat cgtcgcggtg
     1201 ctgcttatcc tcggcatccc ctcgggtttc ctgacctcgg cgatacagga ccgggtcgag
     1261 cgcgaaaccg ggtatcggct gacgatcgcg ggctcgacca agatcgggct gtggccgtcg
     1321 ctgaacgtga gggtcaatga cgtcacgctc gaggcaccga aagaccgcga cgcctctaac
     1381 cgcgtgacgg tcggcagcgt tcaggccgac atgacgctca aaagcgtgtg gtcgggccat
     1441 ccgcaaatca ccgaactcgt catcacccgc ccggtgctgc accacccgtt gctgcgcgaa
     1501 cgcgagcggc tgccggctcc ggctaccagc ccggcggcat cctctgacgg cacggatgcg
     1561 aatgcgctcg cgatcgatcg ggtgacgatt gtcgacggca cgatggtgat gttcaacctg
     1621 cgcgaccgcg tcgaaaatcg catcgaaggc atcaacgccg aagccttgat cggcggagat
     1681 cgcaagatca aggtcagcgg ccgcgcgcgc gccggcgagc agccgctcaa attcgcgata
     1741 acagcgacgg cgcccgcccc gcccctgcag cgccagaaca ttccggtcga gctcacgctc
     1801 gacgcatccg gcctgttgca ggccccgctt gcggccaagg ccgaggtccg gctcaacggt
     1861 tcgatcgtga tgatcaacgg cgtcaccgga acgctcggcg acggcgcctt caatggctgg
     1921 gcatcggtcg atctttccag caagccactg gtcaagctcg atctcgattt tcagcggctt
     1981 gatgtcgcga cgtcgagaga gcgcgccacg tccgcttcgc tgggttcgca accctggagc
     2041 aatgcctcga tcgacctcac cggcctgaat tatgtcgacg cgcaagtgcg ggtttccgct
     2101 gccgagctca acatcggcga agcgcatttc gcgccggttg cgatcgacac ggcggtcgcc
     2161 gccggcgtgc tcaaaaccag cttttcgaat ctcggtgtct atggcggcca ggcttctggc
     2221 cagctcgtcg tcgatgtgac cagcggcagc cctggctatg cgttctccag cgatctcgtc
     2281 ggcgtccgcg ccttgccgct gctgcggagt gctgccgact tcgacaagct cgacggcaag
     2341 atgcaggcga aaatgacgtt gcgctctgcc ggcaacagcc agcacctggt catgtcgaac
     2401 ctgagcggaa cggtcttcgt cgtgttccag gatggcgcga tcagaggcct caacgtcgcc
     2461 gcgatgatcc gctcgctcac ctcgggcacg ctggcgggat ggcaggaggg caaggagcag
     2521 atcaccgacc tcacgcagtt gtcggcctcg ttccgcatcg acaagggcca ggcgaccacc
     2581 gccgatctca atctggtcgg cccgctggtg aagatgaccg ggaccggcac catcgatctc
     2641 gccgcgaaag cgctggcgct ccgggtcgaa ccgaaactcg tgctgaccac cgaaggccag
     2701 ggccgcacgt ccgatccggt cgggctcggc atccccgtgg tgatcgacgg accgtgggcc
     2761 gagccgcgga tctatccgga tatggccggc atcctcgaca atcccgatgc ggcctacgcc
     2821 aagctgaagg aaatgggcaa gggcctgttc ggcagcagtg gcggattggg aagtatcttg
     2881 ggcggattgg gcggatccgg aggaagcgga tcgagcggca gcggatcggg aagcaatggg
     2941 ctgtctgacc agatcggctc gactctcggc aacctgctgc agcaaggcct cggccagcgt
     3001 cgcagtcaaa accgcacgct cgcagcgccc gcatctccca ctccggacgc accgcctgca
     3061 tcggcgcaaa acgatccggc cccggaagtg tcccaggaca gccaacccat gaacgacgtg
     3121 ctgaagcagc tctttaatcg ctaacatcgc actccgtcgc tccgttgctc cctctcccct
     3181 tgtgggagag gggtggccgc aagcgatgac gtcgcgatct ggcacgtgga cgcccttcat
     3241 ctgcaccatg cccgcggccc ctctccccga ccctgcccca caaggcgggg agggagcgat
     3301 aggccggtgg ccgcaaccct cacccccgaa aactgcccaa aatcgtggct gaccacgctg
     3361 gcagaggccc ggtccaggcc ctcaaattgt gctaaacaga actccggctg ggccaatgct
     3421 gttccgccga tagggcaacg gatgcgcaac gcctcgcagg ggtggcgcgc acgagggtct
     3481 ggatgatcgg ggttaaagac aagatctggt tcctccgcga aggcctgttc gccaaatacg
     3541 tcatgtcgct ggtgggcctc gtggtattcg tgctggcggt gaacggcgcg acggagacct
     3601 ggatcagcta ccgcgccacc aagaccacgc tgatcgacgc catgagcgag aaggccgagg
     3661 cgacggcgcg gcggatcgag cagtcgatct ccgagctgga gcggcagatc agctgggtga
     3721 cgcgcgccag cgccaagacc tattccggcg cggtgacgct ggaggatcac cacgccgact
     3781 atgtgcagct cctgaaccag gtgcccgccg tcagccagct cactttgctc gaccagaacg
     3841 gccgcgggca gctgcggctg tcgcgcacca cgatcgcggt cggcagcaag gcggaccttt
     3901 cccgcgacgt cagccttacc gagaccatcg cccggggggg cgtcagctac gcaccggcat
     3961 tttttcaagg ttcgcggccg ctgatgtcga tcgcggtatc gcattcgggc tccgacccca
     4021 gcgcggccgc caccgtcgcc gaaatcgatc ttggcctgct gtcggatttt ctgggcgacg
     4081 cccaggtcgg caagaccggc ttcgcctatg tgatcgaccc aaaaggccag gtgctggcga
     4141 gttccgccag aggccccgac gtggccaagg atctgtcgaa gttgccgcag gtcgccgccc
     4201 tgatccgacc gaacagccag gctttggcat cgggcaccga cgtcgaccgc catgcggtgc
     4261 tgacagcatc gaacgccatt ccaaaactcg gctggttcgt gtgcttcgag cagccgaccg
     4321 cgcaggcgct ggcgccgatc cgcgatcagc tggtgcggat cgcgctgttg atctcgctcg
     4381 ggcttggggt cgcgattctc gccggcaccg tgctggcgcg gcggatgctg gtgccgatca
     4441 ccgcgctgcg caccggcgca agacggctcg gcgccggcga tttcagccat cgcatcgacg
     4501 tgcacaccaa ggacgagctg gaagaactcg ccgaccagtt caactccatg gcgggccaac
     4561 tgcacgagac gtattcgggc ctcgaaacca aggtcgaaga gcgcacccgc gacctcgcgc
     4621 agtcggtcaa tgaactgaag gtg
//
LOCUS       DASWGM010000279         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_156405,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000279 DASWGM010000000
VERSION     DASWGM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156405"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VGA15_08100"
     CDS             <1..308
                     /locus_tag="VGA15_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX9587692.1"
                     /translation="QLREKARADVVSVFILPPSAADLERRLHSRAQDSDQVIRGRMSR
                     ASLEMSHWAEYDYIVINHDIDAAFAEVQSILKAERLKRERRTGLSTFVRELQRQLEK"
     gene            complement(311..1309)
                     /locus_tag="VGA15_08105"
     CDS             complement(311..1309)
                     /locus_tag="VGA15_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587693.1"
                     /translation="MPNRTAKFASAIFAGLLAGTPLTTISHSAAAAEDECLSGPNDKT
                     PGSGHWYYRIDRATKRHCWYQREQGEKPSQITAPNSAASARPVAPKPAPAAQRSVADA
                     HAELPWQSRIEQPRRDRPVPAITADAAVGEINPDVKPPVAEGQQPNMVSRWLGQSDTD
                     PLNNPIFLTSPTPLAGPLPNGAAPISPIANKDAPDTNVSAAPPTQPLPPPAAGQFAAA
                     DLSSPTHSVQMLLAAILGALALAGVMGRAIFKFVGPRSPAKLKGRKRRGAIWKSADGG
                     RTTSNAYSAADALQPRPDFPRDLDQVDDPGDRIAELLRDFSRKGPNGRKARQPSPT"
     gene            complement(1478..2692)
                     /locus_tag="VGA15_08110"
     CDS             complement(1478..2692)
                     /locus_tag="VGA15_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587694.1"
                     /translation="MAWRNRSIPGAVAALGLMLAATPALAQKQYGPGVTDTEVKIGTT
                     TPYSGPASAYSAGAVSATAYFTMINEQGGVNGRKINYISLDDAYSPPKTVEQIRRLIE
                     SDEVLFLVNPVGTATNMAVLKYINQKRVPHLFIGTGATVFNDPEHYPWTMSWTPHYAS
                     EGEIYARYILSAKPDAKIGILSQNDDLGRDYLLGFRRALGDKASAMIVSSLTYNTSDP
                     TIDSQVVSLKASGADVFFIVTVPKFSAQAIRKAYDIDWHPQEFVVSVGSSIAGAIRPA
                     GFEAAKGIISAAYQKDPADPQWRDDPAMQAWNVWMDKYNPRVDKSDYYAPYGYNIGFA
                     VVRLLKQCGDNLTRENIMKQASHLDMELPLLLPGIRLKTSPTDLRPIKQMRLVRFDGQ
                     RYVLFSDVLASE"
     gene            complement(2784..>3351)
                     /locus_tag="VGA15_08115"
     CDS             complement(2784..>3351)
                     /locus_tag="VGA15_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9587695.1"
                     /translation="AYGALKRLVDRPRQRNLLLIGLGGVGMMGLSFAQARFKQQISVA
                     DLSPAAREAALKNGADVAYDPAEADVAKRIIKETEGGFDAIVDFAGNEKSMAFAVAAV
                     ARGGKIVVSGLMGGNFSLPMVQWIYKRMTIEGFMVGTLAEAHELMALARSGKIKPTPM
                     TEEPMDQVQKWIDELRAGKVVGRIVLKN"
BASE COUNT          618 a          992 c         1034 g          707 t
ORIGIN      
        1 agcaattgcg ggaaaaggcg cgggcggacg ttgtcagcgt tttcatcctg ccgccctccg
       61 ccgccgacct ggaaagacgc ctgcactccc gcgcgcagga ttccgatcag gtcatccggg
      121 ggcggatgag ccgcgccagc cttgaaatga gccactgggc cgaatacgat tatatcgtca
      181 tcaatcacga tatcgatgcg gcgtttgccg aggtgcagtc gattctgaag gcggaacggc
      241 tgaagcgcga acgccgcaca ggtttgtcga cgttcgtgcg cgaattgcag cggcaattgg
      301 agaaatagct ctaggttggg ctgggttggc gcgcctttcg gccgttggga cccttgcggg
      361 aaaaatcccg caacaattcc gcaatcctgt caccgggatc atcgacctga tcaaggtcgc
      421 gagggaaatc cggtcgcggt tgcagggcat ccgctgccga ataggcgttt gatgtcgtgc
      481 gaccgccgtc agccgatttc cagatcgcgc cccggcgctt acggccttta agctttgcag
      541 ggcttcgagg gccgacgaat ttgaaaatgg cgcgtcccat cacgccggcc agcgccagcg
      601 cgcctaaaat agcggccaac agcatctgca cggaatgggt tggagacgac aggtcggccg
      661 cggcgaactg accggcagcg ggcggcggca gcggctgagt tggcggggcc gcgctcacat
      721 tcgtgtcggg agcgtccttg tttgcgatgg gactaattgg cgcggcacca tttggcaaag
      781 gaccggccag cggggttgga ctggtcagga aaatcgggtt gttcaaagga tccgtgtccg
      841 actgccccaa ccaccgcgaa accatgttcg gttgttgtcc ctctgcaacc ggtggtttca
      901 catccgggtt aatttcgccg accgctgcat cggcggttat tgcaggaacc ggccgatcgc
      961 ggcgcggctg ttcgatacgc gactgccagg gtagttcggc gtgagcgtcg gcgaccgaac
     1021 gctgcgccgc tggtgccggt ttcggcgcaa ccggacgcgc cgatgcagcc gaattgggcg
     1081 cggtgatttg agaaggcttt tcgccctgtt cgcgctggta ccagcaatgg cgtttggtgg
     1141 cgcgatcgat acgatagtac caatggccgc tcccaggcgt tttgtcgttc gggccggaga
     1201 ggcactcgtc ctccgccgct gcggcgctgt gtgaaattgt cgttagcgga gtccccgcga
     1261 ggaggccggc gaagatcgct gacgcgaatt tcgcggttcg gtttggcatg ggtatctccg
     1321 gcgttacgca acactccgtg ccccaattct taacaaaaac ttgggtcgta atgagccgca
     1381 atttcggaca gcatgaggct taattcaggc ttgcgcttgg atttgcgcga ccgacagttt
     1441 caggagaaac aaaagttgat gctgttgttt tggtgcatca ttcgctcgcg agcacgtcgc
     1501 taaacagcac gtagcgctgg ccatcgaagc gcaccagccg catctgtttg atcgggcgca
     1561 ggtcggtcgg gctggtcttc aggcggattc cgggcagcag caatggcagc tccatatcca
     1621 gatgggaagc ctgcttcatg atgttctcgc gcgtcagatt gtcgccgcat tgcttaagca
     1681 gccggaccac cgcgaaaccg atattgtagc cgtagggggc gtaatagtcg ctcttgtcga
     1741 cgcgcgggtt gtacttgtcc atccagacgt tccaggcctg catggccgga tcgtctctcc
     1801 actgcgggtc ggccgggtcc ttttgatagg ccgccgagat gatgcccttg gccgcttcaa
     1861 acccggccgg cctgatcgcg cccgcgatcg aggagccgac gctgaccacg aattcctgcg
     1921 ggtgccagtc gatgtcatag gctttgcgaa tggcctgcgc ggagaattta ggtaccgtga
     1981 caatgaaaaa tacgtccgcg cccgacgcct tcagcgaaac cacctgggaa tcgatggtcg
     2041 ggtcgctggt gttgtaggtc aacgacgaca cgatcatcgc cgatgccttg tcaccgagcg
     2101 cgcgcctgaa tcccaagaga tagtcgcggc caaggtcgtc gttctgcgac aggataccga
     2161 tcttcgcgtc gggtttcgcc gagagaatat atctggcgta gatctcgcct tccgatgcat
     2221 aatgcggagt ccagctcatg gtccacggat aatgctcggg gtcgttgaag accgtagcac
     2281 ccgtgccgat gaagagatgc ggcactcttt tctggttgat gtattttagc acggccatat
     2341 tggtcgccgt gcccacggga ttgacgagaa acaacacctc gtcgctttcg atcagccgtc
     2401 tgatctgctc caccgttttg ggcgggctgt aggcgtcgtc gaggctgatg tagttgatct
     2461 tgcggccgtt gacgccgccc tgttcgttga tcatcgtgaa ataggccgtc gcggagaccg
     2521 caccggccga ataggccgat gccgggccgc tatagggcgt ggtggttccg atcttgactt
     2581 cggtatcggt gacgccggga ccgtattgct tctgcgcgag ggccggcgtc gcagccagca
     2641 tcaacccaag cgccgcaacc gcgcccggta tcgaacgatt cctccaagcc atcagaagtc
     2701 ctccgctgtt caagcgaggc ttacatggca ctccgcctct tttggtgagg cttaaagatg
     2761 ctctgcctcg aagatgctcc gcctcagttt ttcagcacga tgcggccgac gacttttccg
     2821 gcccgcaact cgtcgatcca tttctggact tggtccatcg gctcttccgt catcggcgtc
     2881 ggcttgatct tgccggagcg cgcgagcgcc atcaactcgt gtgcctcggc cagcgtcccg
     2941 accatgaagc cctcgatggt catgcgcttg tagatccatt gtaccatggg caggctgaaa
     3001 ttaccgccca tcaatccgga aaccacgatc ttgccgccac gcgcgaccgc agcgaccgca
     3061 aaggccatcg atttctcatt gccggcaaaa tcgacgatcg catcgaatcc gccctcggtt
     3121 tccttgatga tgcgtttggc cacgtctgct tccgcgggat cgtaggcgac atcggcaccg
     3181 tttttcagcg ccgcctcgcg cgcggcggga ctcaaatccg caaccgagat ttgctgcttg
     3241 aacctggctt gcgcgaacga aagccccatc atgccgacgc cgccgaggcc gatcagcagc
     3301 agattgcgct gccgcggccg gtcgaccagg cgctttaacg caccgtaggc g
//
LOCUS       DASWGM010000280        10826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2521372,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000280 DASWGM010000000
VERSION     DASWGM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10826
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2521372"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(598..936)
                     /locus_tag="VGA15_08120"
     CDS             complement(598..936)
                     /locus_tag="VGA15_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587696.1"
                     /translation="MRKVLALVLVLHSAAAMAQGSPPTVGGKLLVQVKPRGAPAKPQS
                     IAAKLQACLEIDDATKERLNCYDAIFPPKPNPKAPAAKAVTDCRFAKEEDERLTCFNG
                     FAEKIPKLPQ"
     gene            complement(1236..2477)
                     /locus_tag="VGA15_08125"
     CDS             complement(1236..2477)
                     /locus_tag="VGA15_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587697.1"
                     /translation="MTLRTFPVAILVLICGPALAASSAIRPAPREAPTPPMVFYVAKG
                     APDACGRGCDSWIAVEGQVDSGAALRFRKFLRLIGNRHLPMYFSSPGGNLDQALAMGA
                     MLRERPTVARVARTVVKECGFEAQDGEACLKLKQSGRELHGDLWTHGAMCNSACPYLM
                     LGAATREIAPDAVLAVHSPKVVVNFSGGVPTQQMRATATQRGIERADLMLSRYVLKMG
                     AEGALLDLAKTVKFESMHILTREEIVRFGIDRREFVETPWTFENSTRSMVRKSAVAKS
                     DAGDTFRTLQWRLFCFTTGQFELDFQRQIARAPTFATVSISNGGAKPLYFALPPVKPQ
                     GFEVWGLRMSRVSLQSLAGLPQFDFTETTLAPDGHRLAHPEKFSTEGLAGALDGLLAT
                     CPAQKDMAPLQASGPQDSAAK"
     gene            2746..4788
                     /gene="thrS"
                     /locus_tag="VGA15_08130"
     CDS             2746..4788
                     /gene="thrS"
                     /locus_tag="VGA15_08130"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9587698.1"
                     /translation="MPDKNTPASGFQFSLTNLKPVESSKIALTFPDGAKREFSKNTTG
                     LDIAKGISPSLAKRTVAMALDGVVTDLADPIEHDAKIEFIARDDPRALELIRHDAAHV
                     LAEAVQSLWPGTQVTIGPVIENGFYYDFFRNEPFTPEDFSAIEKKMREIIARDKPFTK
                     EIWTREQTKQVFRDNGEMFKVELVDAIPADQTIKIYKQGDWFDLCRGPHMTSTGKIGN
                     AFKLMKVAGAYWRGDSNNPMLTRIYGTAFAKQEELDAYLKQIEEAEKRDHRKLGRELD
                     LFHFQEEGPGVVFWHAKGWSLFQSIIAYMRRRLEGDYDEVNAPQMLDKSLWETSGHWD
                     WYRENMFAAQSAGDEAEDKRWFAIKPMNCPGHVQIFKHGLKSYRDLPLRLAEFGVVHR
                     YEPSGAMHGLMRVRGFTQDDAHIFCTEDQLAEECLKINDLILSVYADFGFESDIVIKL
                     ATRPEKRVGTDAMWDHAERVLRGLLDKIAARSGNRIKTGVNPGEGTFYGPKFEYVLRD
                     AIGRDWQCGTTQVDFNLPERFGAFYIDADGSKKTPVMVHRAICGSMERFIGILIEHYA
                     GNFPLWLAPIQAVVTTITSEGDEYAKVVAAAARRAGLRVELDLRNEKINYKVREHSLA
                     KIPALLVVGKREAETHSVSIRRLGSEGQTVMPTDEALAALVEEATPPDIKRARLEA"
     gene            4788..4994
                     /locus_tag="VGA15_08135"
     CDS             4788..4994
                     /locus_tag="VGA15_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587699.1"
                     /translation="MEDEQELPAYPIRAYQLIPDPNRPTVVALAIETDQGHSLFLATR
                     EILEDLGKDVLERAAKMPLKKDQA"
     gene            5147..5710
                     /locus_tag="VGA15_08140"
     CDS             5147..5710
                     /locus_tag="VGA15_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX9587700.1"
                     /translation="MPDAIDLLKTRRSIKPREMGEPGPSPAELETILTIGARVPDHGK
                     LTPWRFIVFEGDARVRAGEVIAKVFARKNPQAPPAEIDIEKRRLTDAPLVIAVVSLTK
                     PHPKVPPWEQELSAGASAMNIVTAATALGYGACWLTGWFAFDRDVLDGLGLKADEKLA
                     GFIHIGTPSKPSEDRPRPALSDIVTRF"
     gene            complement(5854..6933)
                     /locus_tag="VGA15_08145"
     CDS             complement(5854..6933)
                     /locus_tag="VGA15_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587701.1"
                     /translation="MTPWGKGIDRFAARGRLLRALVLAAMLIQVLAGPATAQMFSDQP
                     PPVPPGSVPDPGGAISLAPPSGPGSVPTMPAPLTQPSLTQPSLTQPSIAALPPVAAPQ
                     GAAPPGQAVLSLTARYGKDLPAINAGLVWRVFTDRPDENGTFKLIREERGATPNIVLP
                     PGGYVVHVTFGLVSAVRPVTLKSETDREAFLLPAGGLRIEGRVGTSKIPQNQISFAIY
                     KGSQFEVGERAPLVPSVAAGDVALLPEGTYYIISNYGDANSVVRSDIRVQAGKLTDVI
                     ISHRAAVITLKLVGDKGGEALANTAWSVITPGGDVIKESIGAFPRVVLSEGEYRAIAK
                     NEGKVFERPFNVVNGVDGEVEVIAR"
     gene            7155..8207
                     /locus_tag="VGA15_08150"
     CDS             7155..8207
                     /locus_tag="VGA15_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9587702.1"
                     /translation="MLDILIGRGQNGQDSRDKVGLGSIRRPVIGLALGGGAARGFAHI
                     GIIRTLIAHGIVPNVVVGTSIGAVVGGAYAAGHLDTLEDWARSLQPRNILGYLDIRLN
                     GSGLIGGDKLAAQLEAAIGQTAIEELPLKFATVATEVRTGHEIWLTHGRVVDAMRASY
                     ALPGIFSPVLVGDRWLVDGALVNPVPVSAARAFGAEIVIAANLSSDIFTHSTTIYSHG
                     PSAEVSVSLAPDAEIEPPRPKRGFGKFFSPERTMKREFFGSSGRPGISTVMVDAFNIM
                     QDRITRARLAGDPPDLLISPRVGQIGWFDFHRASDLIAHGARAAERAIDSIQEAIHIL
                     APAAAHRPAAAVEKKV"
     gene            complement(8221..8649)
                     /locus_tag="VGA15_08155"
     CDS             complement(8221..8649)
                     /locus_tag="VGA15_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9587703.1"
                     /translation="MTVRAILDSKGHQIQSVEPGTKLSAAIKILSERKIGAVLAMSEG
                     RIEGILSERDIVRVLGERGAAVLDEPISAVMTRKVVSCRQADTVAAIMEMMTLGKFRH
                     LPVVEDGKVVGLISIGDIVKWRVKEFEMEQEALRDYIKTA"
     gene            complement(8856..9644)
                     /locus_tag="VGA15_08160"
     CDS             complement(8856..9644)
                     /locus_tag="VGA15_08160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX9587704.1"
                     /translation="MESPPELPPDGPREPILTLPGALTAYVLLLAVIHVVRMPLPFDI
                     DDVVIQMFGFIPKRYDSTLLAITFPGGTGAKVWTFVTYSLLHANLTHIGFNVLWLLPF
                     GSALARRFGAVRFFLFMAVTAVAGALAHLVTHEHAVAPMIGASASVSGTMAAAIRFAF
                     VRGSFLSFSRGDADAAARVPALSLLRALRNTRVLGFLAVWFGVNIIFGIGSIAIGADG
                     ASVAWQAHIGGFFAGLLLFSLFDPIPRAAPNAADASSSDASERG"
     gene            9995..10303
                     /locus_tag="VGA15_08165"
     CDS             9995..10303
                     /locus_tag="VGA15_08165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX9587705.1"
                     /translation="MNSNGSNQGFVITAFWETKPGEEDSVAGLLRQFLPEARKEAGVK
                     EFQIHQSVTKPQEFFFYEVFSAETAFAAHQQTEHFKKFILGEAIPKLAKRERSQFRFI
                     "
     gene            complement(10345..10590)
                     /locus_tag="VGA15_08170"
     CDS             complement(10345..10590)
                     /locus_tag="VGA15_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587706.1"
                     /translation="MAYRFACLGVRQIAQKRSPLSARQQDQAMSAYATRHDKLAANYL
                     AFIKLASIRIWLRANESAAQSDHDFIALFEHDLRANA"
BASE COUNT         2162 a         3355 c         3340 g         1969 t
ORIGIN      
        1 ccttcgccct tggcttgtca acaatcataa tgaccatcca tcgttggact taattgcgac
       61 cggctaggcg atcgtcaaaa acaggcatca gaatgaaggt gatttcgtaa agctcctgtt
      121 aggacattgc cttgaaattt actcgtttac cctgttcagc ctgtgatgac gtgaatggcg
      181 atgcggcgtt ccaaatggcg gacgtccggt tttggatcgt ccgtagagca tttacagagc
      241 ggcgaactgg aaggggatcg ttccggtttg tgtcaggggt tcggattctc ctcctccgga
      301 cccccgcctt agttggcggc ctctttcagt tcaagtgagc gttcgatgtc gccgattggc
      361 acagcgcgtc atttcgctgc gatgcaacga ctagtcggct atcggggaca aggcggaccc
      421 gggcagtcag tccgatcggc gcatttatga gttcacggcc cagagccgtt tccgtttcga
      481 tagaatcgaa acggggctct ggattcttga tatgacgcgt tttcttgacg cgagccggta
      541 tccaccccgg atcaagtccg agggtaggct tcgctggaaa acgctccagg cgggattcta
      601 ctgcggaagc ttgggaattt tttctgcgaa gccgttgaaa catgtcagcc gttcgtcttc
      661 ttccttcgcg aaacggcaat ccgttacagc cttcgcggcg ggcgccttgg gatttggttt
      721 gggcgggaaa atggcgtcgt agcaattcag ccgctctttc gttgcatcgt cgatctcgag
      781 acaggcctgc agcttcgcgg caatcgattg gggttttgca ggcgcgccgc gcggcttcac
      841 ctggaccagc agctttcctc cgacggtcgg cgggctacct tgcgccatcg cggcggctga
      901 atgcaggacc aggaccaacg caagtacttt tctcatgcca ctttaacctt cgttttcacg
      961 atcgcctcgc caaattcccg ctcaagtctt attcaactgg aggtgaataa gacccaagcg
     1021 ttatattttt gtttaagcat aaatcgtttc ggaaaaccgg tttccaccct cgggtcatct
     1081 agcgcgaaga cgcgcttcgc gcttctgccc gagagcgtgc ttttccggat catgctcgcg
     1141 cccgacaaaa gcatgcgacg aaaatcttgt cgatggtctc ggtgcccgct ggctgcggcg
     1201 caaagagcgt gccgaccgcc aacgacgcgg ggatgctatt tcgccgcgct gtcctggggt
     1261 ccgctcgcct gcaagggagc catgtccttt tgcgccggac aggtcgcgag caggccgtct
     1321 agtgcacccg ccaatccttc ggtggagaat ttctcagggt gtgccagccg gtgcccatcc
     1381 ggcgccaggg tggtctcggt gaaatcgaac tgaggcagcc cggcaagcga ctgtagcgat
     1441 acccggctca tccgcaggcc ccaaacctcg aaaccctgcg gcttgaccgg cgggagcgcg
     1501 aagtacagcg gcttcgctcc gccatttgaa atcgataccg tggcaaaggt cggcgccctt
     1561 gcgatctggc gctgaaaatc cagctcaaac tggcctgtgg tgaagcagaa cagtcgccac
     1621 tgcaaggttc ggaaggtgtc tcccgcgtcg ctcttggcaa cggctgactt gcgcaccatg
     1681 ctgcgggtac tgttttcgaa cgtccacggc gtctcgacga actcgcgccg gtcaatgccg
     1741 aagcggacga tctcttcgcg ggtgaggata tgcatgctct cgaatttgac ggtcttggca
     1801 agatcgagca atgcgccctc ggcgcccatt ttcaggacgt accttgaaag catcagatcg
     1861 gcgcgttcga tgcctcgttg cgtcgccgtg gcgcgcattt gctgggtcgg tacgccgccg
     1921 ctgaaattta ccactacttt cggcgaatgg accgcaagca cggcatcggg cgcgatctcg
     1981 cgggtggccg cgccgagcat gagatacgga catgccgagt tgcacatcgc gccatgcgtc
     2041 cagagatcgc catggagctc gcggcccgac tgcttcagct tgaggcaggc ctcgccatcc
     2101 tgggcttcga agccgcactc cttcaccacc gttcgagcaa ccctggcgac ggtgggccgt
     2161 tcgcgaagca tggcgcccat cgccagcgcc tgatcgagat tgccgccggg cgaggaaaaa
     2221 tacatcggca gatggcgatt gccgatcagc cgcagaaatt ttcgaaagcg gagcgccgcg
     2281 ccggaatcga cctggccttc gaccgcgatc cagctgtcgc agccgcggcc gcacgcatcg
     2341 ggcgcgcctt tggcgacata gaacaccatc ggtggcgtcg gcgcctcccg gggcgcaggc
     2401 cggatggcgg aggaagcggc cagtgccggt ccgcagatca acaccaggat ggcgacggga
     2461 aatgtacgca gcgtcatggg cccggggcgt tacgcgattg cgaagaagac cgctggacaa
     2521 cgacgaaaag ctaacaagcc cgttgtgcca agtccatggc ggccccgcgg ggacctcgcg
     2581 aatttcatcg ggtggtcaca tgatggaaca acagtttcgt gcaaagccgc ctatcgcaac
     2641 ccgattttct tgagggatcc ggattgcgtc acgccaatcc cctgctatac cgctgcttcc
     2701 cgcttacctg cgctcgttcg caggagtgag ccacaggaga cactaatgcc cgacaagaac
     2761 acgcccgcgt caggattcca gttcagcctc accaatctga agccggtcga atcctcaaag
     2821 atcgccctca ccttccccga cggcgcgaaa cgcgaattca gcaaaaacac caccggcctg
     2881 gatatcgcca agggcatctc gccgtcgctg gccaagcgca ccgtggcgat ggcgctcgat
     2941 ggcgtggtca cggatctcgc cgacccaatc gagcacgatg cgaaaatcga attcattgcc
     3001 cgcgacgacc cgcgcgcgct ggaactgatc cggcatgacg ccgcccatgt gctggccgaa
     3061 gcggtgcaat cgctgtggcc gggcacgcag gtgacgatcg gcccggtgat cgagaacggt
     3121 ttttattacg atttcttccg caacgaacca ttcacgccgg aagatttctc ggcgatcgaa
     3181 aagaagatgc gcgagatcat cgcgcgcgac aaacccttca ccaaggaaat ctggacgcgc
     3241 gaacagacca aacaggtgtt ccgcgacaac ggcgagatgt tcaaggtcga actggtcgac
     3301 gcgatcccgg ccgaccagac catcaagatc tacaagcaag gcgactggtt cgatctgtgc
     3361 cgcggtccgc acatgacgtc gaccggcaag atcggcaacg cgttcaagct gatgaaggtc
     3421 gcgggcgcct attggcgcgg cgacagcaac aatccgatgc tgacgcggat ctacggcacg
     3481 gcgttcgcca agcaggaaga gctcgacgct tacttgaagc agatcgagga agccgagaag
     3541 cgcgaccacc gcaagctggg acgcgagctc gacctgtttc acttccagga ggaaggcccc
     3601 ggcgtggtgt tctggcacgc caagggctgg tcgctgttcc agtcgatcat cgcctatatg
     3661 cgccggcgtc ttgaaggcga ttacgacgag gtgaacgcgc cgcagatgct cgacaagtcg
     3721 ctgtgggaaa cctcaggcca ctgggactgg tatcgcgaga acatgttcgc cgcgcagtcg
     3781 gccggcgacg aagccgaaga caagcgctgg ttcgccatca agccgatgaa ctgccccggc
     3841 catgtgcaga tcttcaagca cgggctgaag agctaccgcg atctgccgct gcggctggcc
     3901 gaattcggcg tggtgcatcg ctatgagccg tcgggcgcga tgcatgggct gatgcgggtg
     3961 cgcggtttca cgcaggacga cgcacacatc ttctgcaccg aggaccagct tgcggaagag
     4021 tgcctgaaga tcaatgatct catcctctcg gtttacgcgg acttcggctt cgagagcgac
     4081 attgtgatca aactcgcgac gcggccggaa aaacgcgtcg gcaccgatgc gatgtgggac
     4141 cacgccgaac gtgtgctccg tgggctgctt gacaagatcg cggcgcgatc cggcaaccgc
     4201 atcaagaccg gcgtcaatcc gggagaaggc accttttacg gcccgaagtt cgaatatgtg
     4261 ctgcgcgacg ccatcggccg cgactggcag tgcggcacca cgcaggtcga ttttaatctg
     4321 ccggaaagat tcggcgcctt ttacatcgac gccgacggct cgaagaaaac gccggtcatg
     4381 gtgcaccgcg cgatctgcgg ctcgatggag cgttttatcg gcatcctgat cgaacactat
     4441 gcgggtaact ttccgctttg gctggcgccg atccaggccg tcgtcaccac catcacgtcg
     4501 gaaggcgatg aatacgccaa ggtggtcgcg gctgcggcgc gtcgcgccgg cttgcgcgtc
     4561 gaactcgacc tgcgcaacga aaagatcaac tacaaggtcc gcgaacattc gctggcgaag
     4621 attccggcac tgctggtggt cggcaagaga gaggccgaga cccattcggt ctcgatccgg
     4681 cgcctgggca gcgaaggcca gaccgtgatg ccgaccgatg aggcgctggc cgcgctggtc
     4741 gaggaagcga ccccgccgga catcaagcgg gcgcggctgg aggcgtgatg gaagacgaac
     4801 aggaactgcc ggcctatccg atccgggcct accagctgat cccggatccc aatcggccga
     4861 ccgtggtggc gctggcgatc gagaccgatc agggccacag cctgttcctg gccacgcgcg
     4921 aaattctgga agatctcggc aaggatgtgt tggagcgggc ggcgaagatg ccgctcaaga
     4981 aggatcaagc ctagcggttg gatcggccgt atcgtttgcg tgccggcctc gccgaggcgc
     5041 aaaccatctc ccgatcacaa tcggtctaaa atgcccgtcc accgctcgtg gccggcggca
     5101 ccgcgtgcta tgcgatcccc cagcattccc gatgaaagag cccgcattgc ctgacgccat
     5161 cgacctcctc aaaacccgcc gctcgataaa accgcgcgag atgggcgagc ccggcccctc
     5221 gccagccgag cttgaaacca ttctgacgat cggtgcgcgg gtgcccgatc acggcaagct
     5281 gacgccgtgg cgttttattg tgttcgaggg cgacgcgcgc gtccgcgccg gcgaggtgat
     5341 cgcaaaggtg ttcgctcgga aaaacccgca agccccaccc gccgagatcg atatcgagaa
     5401 acgccggctc accgatgcgc cgctggtgat cgccgtggtc agcctgacaa aaccgcatcc
     5461 caaggtgccg ccctgggagc aggaattatc cgcgggcgcc agcgcgatga acatcgtcac
     5521 cgccgccacc gcgctcggct acggcgcctg ctggctgacc ggctggttcg cgttcgaccg
     5581 cgacgtgctc gacgggctgg gattgaaggc cgacgaaaaa ctcgcgggct tcatccatat
     5641 cggcacgcca tcgaagccga gcgaagaccg cccgcgcccg gctttgtcgg atatcgtgac
     5701 gcggttttga caatgctgtc attgccgggc gtagtagtct gcctggcgca gactacgtaa
     5761 acttgtctgc gctcccggca atccatcatt gaaaaaacac gtgtgaagat tgatggatgc
     5821 ccggatcaag tccacgggca tgacggcgtt ggatcagcgc gcgatgacct cgacctcgcc
     5881 gtcgacgccg ttgacgacgt tgaaggggcg ctcgaacacc ttgccctcgt tcttggcgat
     5941 ggcgcgatat tcgccttcgg acagcacgac gcgggggaat gcgccaatcg attccttgat
     6001 gacgtcgccg cccggcgtaa tcaccgacca cgcggtgttg gccaaggcct cgccgccctt
     6061 gtcgccgacg agcttcagcg taataaccgc ggcgcgatgg ctgatgatca cgtcggtcag
     6121 cttgccggcc tgcacgcgga tatccgagcg caccacggag ttggcgtcgc cgtagttgga
     6181 aatgatgtag taggtcccct ccggcaatag cgcgacatca ccggcggcga cgctcggcac
     6241 caagggcgcg cgctcgccga cttcaaactg gctgcccttg tagatcgcga acgaaatctg
     6301 gttctgcggg atctttgacg tgccgacgcg gccttcgatc cgcaggccgc ccgccggaag
     6361 caggaaggcc tcgcggtcgg tttcggactt cagggtcacc ggccgcaccg cgctgaccag
     6421 tccaaaggtg acatggacga catagccgcc cggcggcagc acgatgttgg gcgtcgcacc
     6481 gcgttcctcg cggatcaatt tgaaggtgcc gttctcatcg ggacgatcgg taaaaacccg
     6541 ccacaccagc ccggcgttga tggccggcaa gtccttcccg taacgcgccg tcagcgacag
     6601 cacggcctgg ccggggggcg ctgcaccctg aggcgccgca accggtggca gcgcggcgat
     6661 cgaaggttgc gtcagggaag gctgcgtcag ggaaggctgc gtcaacggcg ccggcatcgt
     6721 gggtacggat cccggaccgg acggcggggc caggctgatg gcgccgccgg ggtcgggaac
     6781 cgatcccgga ggcactggtg gcggttgatc ggagaacatc tgggccgtag ccggacccgc
     6841 cagcacctgg atcagcattg ccgcaagcac aagcgctcgg agcaagcgtc cgcgcgccgc
     6901 gaatcggtcg atgcctttac cccatggtgt catggaacat gcttttcacc gaaaacgcgg
     6961 caaattcaag cctgcaggcg cggcagatgg cggctccacg gaaccggcaa aaacccccga
     7021 tgctatcccg agcgtcgcgg tccagtcaca atccctgcct acgcccatcg gcaccgcagc
     7081 ataaaccatt cttgtggtac gttctggcgg gaccgggctt tagtgcatag tcttgtgctc
     7141 ccaggagtta tcaggtgctg gatatattga tcgggcgcgg ccaaaacggc caggacagtc
     7201 gcgacaaggt gggtctcggg agtatccggc ggccagtaat cgggctcgca ctgggcggcg
     7261 gcgccgcccg cgggttcgcc catatcggca tcatcaggac attgatcgcc cacggcatcg
     7321 tccccaatgt ggtggtcggc acctcgatcg gcgcggtcgt tggcggcgcc tatgccgcgg
     7381 ggcatctcga tacgctggaa gactgggcgc gcagcctgca gccacgaaat atcctgggct
     7441 atctcgatat ccggctcaac ggttcgggcc tgatcggcgg cgacaagctg gcggcccagc
     7501 tggaagccgc gatcggccag accgcgatcg aggaattgcc gctgaaattt gcaaccgtcg
     7561 cgacggaagt ccgcaccggg catgagatct ggctgaccca cggccgggtc gtcgatgcga
     7621 tgcgcgcctc ctatgcgctg cccgggatat tttcgccggt gctggtgggc gaccgctggc
     7681 tggtcgacgg cgcgctggtg aatccggtcc cggtgtcggc cgcgcgggcg tttggcgcgg
     7741 aaatcgtcat cgccgccaac ctctcaagcg acattttcac ccacagtacg acgatctact
     7801 cgcacggccc gtcggccgag gtatcggtca gcctcgcgcc ggacgccgag atcgagccac
     7861 cgcggccgaa acgcggcttc ggcaaattct tctcgcccga gcgcaccatg aagcgcgagt
     7921 ttttcggcag cagcggacgg ccgggcatct ccaccgtcat ggtcgatgcc ttcaacatca
     7981 tgcaggaccg cattacccgg gcgcgcctgg ccggcgatcc acccgacctg ttgatctcgc
     8041 cccgggtcgg ccaaatcggc tggttcgatt tccaccgcgc cagcgatctg atcgcgcatg
     8101 gcgcgcgcgc cgccgagcgc gccatcgatt ccatccagga agcgattcac attctcgcgc
     8161 cggccgcggc ccaccgaccc gccgctgcgg ttgagaagaa ggtctgagcg aacgcgtccg
     8221 ctaggcggtc ttgatgtaat cgcgcagcgc ttcctgctcc atctcgaatt ccttgacacg
     8281 ccatttgacg atgtcgccaa tcgaaatcag gccgaccacc ttgccgtcct cgaccaccgg
     8341 cagatgcctg aatttaccca gggtcatcat ctccatgatg gcggcgacgg tgtcggcctg
     8401 cctgcaactg acgaccttgc gcgtcatcac cgcgctgatc ggttcgtcca gcaccgcagc
     8461 accccgctca cccagcacgc gcacaatatc gcgctccgac aggatgcctt cgatgcgacc
     8521 ttcgctcatc gccagcacag cgccgatctt gcgctccgac agaatcttga tcgctgccga
     8581 aagcttcgtg cccggctcga cgctctggat ctgatggcct ttggaatcga gaattgcgcg
     8641 taccgtcatg gtcgtctccc taaatggttg tctccctgaa gccgatcgcg cccttgagcg
     8701 agcgcggtcg tggttgcctt ttgagtctgc aatcaacagt tttagcgccg attggttgca
     8761 ggcgcttcgt aagccgcgga tattaaagca cgctcacggg cttatgcact aactctgaca
     8821 tgatggatga aaccgacttg cgccgcaagc ggcgatcagc cgcgttccga tgcatcggag
     8881 gacgacgcat ctgcagcatt gggcgcggca cgcgggatcg ggtcgaacag cgaaaacaac
     8941 agtagccccg cgaagaaccc gccgatatgc gcctgccagg cgacgctggc gccgtcggcg
     9001 ccaatcgcga tcgatccaat gccgaagatg atgttgacgc cgaaccagac ggcaagaaaa
     9061 cccaggacgc gcgtgttgcg caacgcgcgc agcagcgaca gcgccggcac ccttgccgcg
     9121 gcatcggcgt cgccgcggct gaatgacaga aaactgcccc gcacgaaggc gaagcggatc
     9181 gcggctgcca tcgtgccgga caccgacgcc gacgcgccga tcatcggcgc caccgcgtgt
     9241 tcatgggtga cgagatgcgc cagcgcgccg gccacggcgg tcaccgccat gaacaggaaa
     9301 aagcgcaccg cgccaaagcg gcgcgccagc gcgctgccga acggcagcag ccacagcacg
     9361 ttgaagccga tatgggtgag attggcgtgc agcagcgaat aggtcacgaa ggtccagacc
     9421 ttggcgccgg tgccgccggg aaacgtgatc gccagcagcg tcgaatcgta gcgcttcgga
     9481 atgaagccga acatctggat gacgacgtcg tcgatatcga acggcaacgg cattcggacg
     9541 acgtgaatca ccgcaagcag caggacatag gcggtcaagg cgccaggcag cgtcaggatc
     9601 ggttcgcgcg ggccgtccgg cggcaattct ggcggggatt ccaagggctc aaacacctca
     9661 aacggctggc tcacgtgacc gattgcgccc cattaggcgg cttggcgctg cggcgcaagg
     9721 gcgcaaaccc gtccgggggg cgcggtggcc gaatgcatcg atcgccccca gaaccgtcct
     9781 ccatcgccaa agataccatc ttttggattt tccgtccgaa atgcccggca gcccggcaag
     9841 cgcggcagcc cggcaagcgc gggatcactt ccgcggcctg tttggggtga cgttggttcg
     9901 gcgcgcaccg gagcattcgg accagggggc gtgccaatct ccgccggatc gccccttgag
     9961 gcgaatggga cgtcagcatc gacggagaaa gaccatgaat tcgaatggct ccaaccaggg
    10021 tttcgtcatt accgcatttt gggaaaccaa gccgggcgag gaggattccg ttgcaggctt
    10081 gttgcgacaa tttcttcccg aggcgcggaa agaagcgggc gtgaaggaat tccagattca
    10141 tcagagcgta accaaaccgc aggagttctt cttctacgaa gtattttcgg cggaaaccgc
    10201 gttcgctgcc caccagcaga ccgagcattt caagaaattt atcctcggcg aggccatccc
    10261 gaagcttgcc aagcgtgaac ggtcgcagtt tcgatttatc tgagccgtgt cacgcacaga
    10321 gcatgatccg cctcgggaca aacgctaagc gtttgcccgg agatcatgct caaacaaagc
    10381 gatgaagtca tgatccgatt gggctgcgga ctcattagcg cgcagccaaa tgcggattga
    10441 tgcgagtttg atgaaggcca gataattggc cgcgagcttg tcatgtcggg tcgcatacgc
    10501 cgacattgct tgatcttgtt gccgggctga aaggggcgag cgcttttgcg cgatttgacg
    10561 cacccccagg caggcaaatc tgtaagccat aacagaaaca tgaactcaac cgagtccgta
    10621 ctgcaacccg ttcgctcctc aaggctgcgg gcctttatcc ttgcccagat gttcggcgag
    10681 gcatagcgtt tggtggcggc aaagagtttg tcgtgtgaac caagccatca acagcgatta
    10741 cgatggcatc ggtcaagtcg ttggccacaa gaaagggacg tacaagatca tcaaaacgaa
    10801 ctgatgcagc cgaccgaagg aggtct
//
LOCUS       DASWGM010000281        11333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2245098,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000281 DASWGM010000000
VERSION     DASWGM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11333
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245098"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VGA15_08175"
     CDS             complement(<1..396)
                     /locus_tag="VGA15_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX9587707.1"
                     /translation="MSGVHDFTAKSLAGEDIPLKRFEGQVLLIVNTASACGFTPQYKG
                     LETLHQALNPRGFSVLGFPCNQFGSQEPGDSRQIEQFCTSNYAITFPMFAKIDVNGTG
                     AHPLFNYLKSAKSGLLGSSIKWNFTKFLVD"
     gene            complement(429..1829)
                     /locus_tag="VGA15_08180"
     CDS             complement(429..1829)
                     /locus_tag="VGA15_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9587708.1"
                     /translation="MEDLWRLSAADIAALIRSKKVSAKQTASAALARLDAVNPSINAV
                     VDHRPEDVLAQAAAIDAAIARGEAVGPLAGVPVTVKVNIDQQGFATTNGLKLQRDVIA
                     KTNNPVVDNLRKAGAVILGRTNCPAFSYRWFTTNLIHGDTKNPRDASITPGGSSGGAG
                     AAVAAGIGHIALGTDIAGSIRYPAYACGVHGLRPTVGRIAAFNASLPERPIGPQISAV
                     SGPLARTVGDLRMALAAMSAHDARDPWWVPAPLEGPPMPKRAALCLRPDDLETVAEVK
                     AAVADAGKRLERAGWVVEEIEDTPPLREAADLQTKLWLGDGFEAQLEAAEREGDPGAL
                     ACLRGNRAKVFPFDAAAYSKALTRRATLAREWLAFFETYSVLLMPVSAELPFPDGLDL
                     RDDVSFTRVWRAQLPQIAIPFMGLPALTVSTGLLGRVPVGVQLVSTRYREDLCLLAGE
                     AIEAGGTPSAPVDPVS"
     gene            complement(1914..2480)
                     /locus_tag="VGA15_08185"
     CDS             complement(1914..2480)
                     /locus_tag="VGA15_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9587709.1"
                     /translation="MRKIIAALAFAFIATSVNAETIAERAAPCLACHGEKGQSETENT
                     PSLGAQQAPYALIQLFMFREKLRVFEPMNEMAKALSDDDLRTFSDFLATLPKPAPPAD
                     TGDPARLQRALALVQQHRCNSCHNTDFSGKDNVPRIADQREDYLAKTLREYKDNSRHG
                     YDATMAEVLQPVTPEQIADLAYFLARVR"
     gene            complement(2787..4055)
                     /locus_tag="VGA15_08190"
     CDS             complement(2787..4055)
                     /locus_tag="VGA15_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9587710.1"
                     /translation="MMICSSAPRSVLLAGAFVGVFACVAVAQQPADPAPAATAAPALP
                     PGSPLIGRPASEAAAKLAPVAPPPIPAAPDKLPTAKLKVPAGFNLEVYAAGMANARSL
                     AEGDKGTVFVGSRLVDKVYAVVNKDGKRSVKVLASGLYRPNGVAFKNGTLYIAELSKV
                     SKIDKVEEVIDNPPKPTMIYDNLPKDEAHGWKFIGIGPDNKLYVPVGQPGNNVLHDDA
                     HGQIRRINLDGTGAEVVALGVRNSVGFDWHPETKQLYFTDNGRDWMSEDVPQDELNRV
                     TKAGEDFGAPYCYQGNISDPEFGWGHSCSEFTAPVGLMGPHAAALGMRFYTGGMFPKS
                     YKNAIFVARHGSWNRSKKFGGDVVVVKLNKDGTVKSVEPFITGFLEDNKYVGRPVDVL
                     PMKDGSLLVSDDWNGAVYRISYGKPKVAAR"
     gene            complement(4269..4526)
                     /locus_tag="VGA15_08195"
     CDS             complement(4269..4526)
                     /locus_tag="VGA15_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587711.1"
                     /translation="MPRYFFNTQIGDELISDPEGEELRDLDRAWEVARAMIRELLKTE
                     GAERGLLSAILEVTDDEGEVVLEFPFAEAMLDMTDGPVTKH"
     gene            4825..4905
                     /gene="pqqA"
                     /locus_tag="VGA15_08200"
     CDS             4825..4905
                     /gene="pqqA"
                     /locus_tag="VGA15_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HEX9587712.1"
                     /translation="MAWKTPKIVEVPVGMEINMYACAARK"
     gene            5063..5992
                     /gene="pqqB"
                     /locus_tag="VGA15_08205"
     CDS             5063..5992
                     /gene="pqqB"
                     /locus_tag="VGA15_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEX9587713.1"
                     /translation="MLRVVVLGAAAGGGVPQWNCGCPVCRIARTEHPELQSTQASIAI
                     SADGDHWFLINASPDLRQQLIATPQLHPKAGRLRHSPISGVILTNGEIDAVAGLLSMR
                     EGWPFTIYAHTRVLSILNSNSIFNVLNEKNVRRRPIEVSQTFEPTLPDGSPSGIEILP
                     FAVPGKSAWYLEGKDHPAGGDGAGDTLGLRVSDRATGKYLYFLAACAHVTDDLKSRLG
                     GASLIFFDGTVWRDDELITAGLGNKTGQGMGHIAMSGTTGAIAALAGLDIDRKIFLHI
                     NNSNPALLRDSAEREIVERAGWQIPADGTEITL"
     gene            6013..6768
                     /gene="pqqC"
                     /locus_tag="VGA15_08210"
     CDS             6013..6768
                     /gene="pqqC"
                     /locus_tag="VGA15_08210"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEX9587714.1"
                     /translation="MTAFSRGGSPPLNSAEELEAALRHIGATRYHNLHPFHRLLHGGK
                     LNKGQVQAWALNRYYYQSTIPIKDAVVISRFRDRGIRLEWRHRIEDHDGDLGTEGGIE
                     RWLKLTEGLGLDSTYVESTEGILPATRFAVEAYVHFVRDKTPLEAIASSLTELFAPNL
                     HEERISGMLAHYDFVNPDIMSYFSRRMQQAPRDANFALDYVKTHANTPEQREAVCNAL
                     LFKTNVLWVQLDALYHAYVDGHVPPGAFVPKAS"
     gene            6808..7125
                     /gene="pqqD"
                     /locus_tag="VGA15_08215"
     CDS             6808..7125
                     /gene="pqqD"
                     /locus_tag="VGA15_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEX9587715.1"
                     /translation="MAGLRSRNISVSEASRPVLPRHAKLRFDETRQRWVILAPERVLA
                     PDDVAVEVLQLCDGVRSVEAMIDLLAEKYAADRAAIATDVIAMLQDLADKGFLTEARE
                     KTS"
     gene            7122..8330
                     /gene="pqqE"
                     /locus_tag="VGA15_08220"
     CDS             7122..8330
                     /gene="pqqE"
                     /locus_tag="VGA15_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEX9587716.1"
                     /translation="MSPTLTDAAVAASDPSDGLAVLEQRGSTAETFGIPLAVLAELTH
                     RCPLQCPYCSNPVELERGGSELTTAEWKKVLTELAEIGVLQIHFSGGEPTARKDLVEL
                     VQHASDVGLYSNLITSAVLLTRERLAALAEAGLCHVQISFQGNEPVVADRVAGLKNAH
                     EKKIEVAKWTRELDLPLTVNAVMHRQNLHQLPDIIQMAVDLDADRLEVANVQYYGWAL
                     KNRAALMPTLEQIEETSRIVEEAEARLKGILAIDYVVPDYYALRPKKCMGGWGRQFFN
                     ISPAGKILPCHAAETITGLEFLSVRSNHSIAWIWQNSEAFNRYRGTGWMPEPCKSCEF
                     REIDFGGCRCQAFALTGDAANTDPACTLSPLHEQIFKLAESEAAADNKRFIYRNFAGG
                     TLEAGGDHGA"
     gene            8320..9309
                     /gene="pip"
                     /locus_tag="VGA15_08225"
     CDS             8320..9309
                     /gene="pip"
                     /locus_tag="VGA15_08225"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX9587717.1"
                     /translation="MAPDADAGKASGNRSDPFAPLTSQLLPVGDGHEIYVETVGRADG
                     IPAVYLHGGPGSGCQPDHRRLFDPERFHAVLFDQRGAGRSRPKGRREHNTLPHLIADM
                     EIIREKSGFERWIIVGGSWGATLALAYAQAHPNRVTGIVLRATFLGTRAELEGAFLDA
                     LPRFYPDLSDDFLKLLPPEERAQPLDAYWRRILDSDPAVHGPAAHAWGDTERILSGHV
                     SGRVRLDPHSPNSTRALPATPFMEAHYFQNNCFMRPGQLLAEAHKLAGIPAIIVQGRY
                     DLLCPPSMSHALAAVWAGSEVRSVEGAGHQLYDPGVRDAVMKAIADLTSKITR"
     gene            9323..9997
                     /locus_tag="VGA15_08230"
     CDS             9323..9997
                     /locus_tag="VGA15_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587718.1"
                     /translation="MPLAGKGMLLTSMDIDPSDEVEFNRWYDREHLLERVAIDGFLEA
                     RRYVAHAGSPKYLCLYSTATFEVLDSPTYRTVLMNPTDWSKTTLARFKNMIRAIARIT
                     VSRGAGRGAALGIVRLRPAGDKGKLRTALRDRLDPEKLDGIISMHLIESDPALSRPIT
                     DDPPVSDPGSGDWFVLIDATDVRAVSAAITDRLGDVPAPMAAETISTGVYKLMWDLAK
                     SDIRSS"
     gene            10252..>11333
                     /locus_tag="VGA15_08235"
     CDS             10252..>11333
                     /locus_tag="VGA15_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9587719.1"
                     /translation="MKPTDISAPDYFHKVVDCQWACPAHTPVPEYIRLIAEGRYSDAY
                     MINWRSNVFPGILGRTCDRPCEPACRRGRVEETPVAICRLKRVAADFKDDVKHRMPRP
                     SPKNGKRIAIVGGGPASLTVARDLAPLGYHCTVFDSDPKAGGMMRTQIPKFRLPDSVI
                     DEETDYILNLGIEFKGGTRIDSLKKLLGENFDAIFVGSGAPRGRELKIPGRKEAASHI
                     HIGIDWLSSVSFGHVEKIGKRVIVLGGGNTAMDCCRTARRLGGQDVKVIVRSGFEEMK
                     ASPWEKEDALHEDIPILNFMVPKAFTHDNGKLTGVTFQKVKAEYDDKGRRNLVPAGDP
                     DQTIACDDVLVAVGQENAFPWIERDCG"
BASE COUNT         2226 a         3518 c         3450 g         2139 t
ORIGIN      
        1 atcgaccagg aatttggtaa aattccattt gatcgacgag ccgagcaacc cggacttcgc
       61 actcttcaga tagttaaaca acgggtgcgc gcctgtgcca ttgacgtcga tcttggcaaa
      121 catcggaaaa gtgatggcat aattgctggt gcagaactgc tcgatttgcc gggagtcgcc
      181 gggttcctgg cttccgaact ggttacacgg aaacccgaga acggaaaagc cacgtgggtt
      241 aagcgcctga tgcaacgtct cgaggccctt gtattgcggg gtaaatccgc acgcgctcgc
      301 ggtgttgacg atcaggagca cctgaccctc gaaccgcttc aggggaatgt cctcgccggc
      361 aagcgatttt gccgtgaaat catgaacgcc ggacatcggt tttctttcag cttgaaacgg
      421 aaattcactt aactcacggg atcgaccggc gccgacgggg ttccgccagc ttcgatcgcc
      481 tcgccggcca gcaaacacag atcctcgcgg tagcgggtgg agacgagctg aacgccgacg
      541 ggcacacgcc ccagcagtcc tgtggagacg gttagcgcag gcagccccat gaagggaata
      601 gcaatttgcg gcaattgcgc ccgccaaacc cgcgtgaacg agacgtcgtc gcgcagatcg
      661 aggccgtcgg ggaacggcaa ctcggcggag accggcatca gcagcaccga ataggtctcg
      721 aagaacgcca gccattcgcg cgccagtgtc gcccgccgcg tcagcgcctt cgaatacgcg
      781 gccgcatcga atgggaaaac cttggctcgg ttgccgcgca agcacgccag cgcgccggga
      841 tcgccctcac gctcggcggc ctccaattgc gcctcaaagc catcgccaag ccacaacttg
      901 gtttgcaggt ccgcggcctc ccgcagcggc ggcgtgtctt cgatctcttc gaccacccat
      961 cccgcgcgct ccaggcgctt gccggcgtcg gcgacggccg ccttgacttc agcgacggtc
     1021 tcaagatcgt cgggacgaag acacagtgcg gcccgcttgg gcattggcgg cccttccagc
     1081 ggcgccggga cccaccaggg atcgcgcgcg tcatgggcgg acattgccgc cagcgccatc
     1141 ctgaggtcgc cgacggtgcg cgcgagcggg ccggataccg cagagatctg cggcccgatc
     1201 ggacgttccg gcagtgacgc attgaaggct gcgatacgac cgacggtagg ccgcaggcca
     1261 tgaacaccgc aggcataggc gggatagcgg atcgagcccg cgatatcggt gccgagcgcg
     1321 atatgcccga tgcccgcggc gaccgcggca cctgcgccgc cggacgagcc tcccggcgtg
     1381 atgctcgcat cgcgcggatt cttggtgtcg ccatggatca gattggtggt gaaccagcgg
     1441 tacgaaaatg ccgggcaatt ggtgcgtccg aggatcaccg cgccagcctt gcgcagattg
     1501 tccaccaccg gattattggt cttggcgatg acatcgcgct gcagcttgag gccgttggtg
     1561 gtggcaaacc cttgctggtc gatattgacc ttgacggtga ccggcacgcc cgccagcggg
     1621 cccaccgctt cgcctcgcgc gatcgccgca tcgatggccg cagcctgcgc cagcacgtct
     1681 tccgggcggt gatcaaccac ggcgttgatc gacgggttga ccgcatccaa tcgcgccagc
     1741 gctgccgatg cggtctgttt cgccgaaact tttttcgacc ttatcagggc ggcgatatcc
     1801 gcagccgaca gacgccaaag atcttccatt gccaactccg tttgaatgcc cgtcttttag
     1861 cgctgcctac aggccggcgc catcgggaaa caacggtgtc agcgactaca agctcagcgc
     1921 acgcgggcga gaaaataggc aagatcggcg atctgctccg gcgtcaccgg ctgcagcacc
     1981 tccgccatgg tggcatcgta gccgtggcgg ctgttgtcct tgtattcccg caaggtcttg
     2041 gcgagatagt cctcacgctg gtcggcaatg cgcgggacgt tgtctttgcc ggaaaagtcg
     2101 gtgttgtggc aggaattgca gcgatgctgc tgcaccagcg ccagggcgcg ctgcagccgc
     2161 gccggatcgc cggtatcagc cggcggcgcc ggcttcggca aggtggcaag aaaatcggag
     2221 aacgtgcgaa ggtcgtcgtc gctgagcgcc ttcgccatct cgttcatcgg ctcgaacacg
     2281 cgcagctttt cacgaaacat gaaaagctgg atcagcgcgt aaggcgcctg ctgcgcgccg
     2341 agcgacgggg tattttcggt ttcggattgt cccttttcgc catgacaggc aaggcacggc
     2401 gcggcacgct cggcgatcgt ctccgcgttg acggaagttg cgatgaaggc gaacgccaaa
     2461 gccgcgatta ttttacgcat tactggtccc ggcggcgacg atcaagaatt gaaacgtttt
     2521 cgtcattgcg agttccgtac cggccttggg gccgaccctg atggatgaaa gtgtggtgaa
     2581 tcaaggcgat agggctcggg gagttttccc actttcttcc taggagcaac gcgacgaaga
     2641 tccagctttt tggtgcagct atggattgct tcgcggagcc agtcatcggg cggcgcggcg
     2701 cctgtcatcg ggcgcgcatt cgcgcgaccc gttggcgccg acccgtcggc tcgcaatgac
     2761 gaattcaagt tttgttgcta ccgattttac cgcgcggcca ccttcggctt gccgtagctg
     2821 atgcgataga cggcgccgtt ccagtcgtcg gagaccagca acgaaccgtc cttcatcggc
     2881 agcacgtcga ccggccggcc gacatatttg ttgtcttcca ggaagccggt gatgaacggc
     2941 tccacggact tgaccgtgcc gtccttgttc agtttgacga caacgacatc gccgccaaat
     3001 ttcttcgacc tgttccatga accgtgccgc gccacgaaga tggcgttctt gtaactcttt
     3061 ggaaacatgc cgccggtata aaaccgcata ccgagcgcgg ccgcatgcgg acccatcaat
     3121 ccgaccggcg ccgtgaattc gctgcaggaa tgtccccagc cgaattcggg atccgagata
     3181 tttccctgat aacagtaagg tgcgccgaaa tcctcacccg ctttggtgac gcgattaagt
     3241 tcatcctgag gaacatcctc cgacatccag tcgcgtccgt tgtcggtgaa gtagagctgc
     3301 ttggtttccg gatgccaatc gaaaccgacg gagttgcgaa cgccaagcgc cacgacctcg
     3361 gccccggtgc cgtcgagatt gatccgcctg atctggccgt gcgcgtcgtc atgcaacacg
     3421 ttgttgccgg gctggccaac cggaacgtac aatttgttgt caggcccgat gccgatgaac
     3481 ttccagccat gggcttcgtc cttgggcagg ttgtcgtaga tcatggttgg ttttggcgga
     3541 ttgtcgatga cctcctcgac cttgtcgatc ttggacacct tcgagagttc ggcgatgtag
     3601 agcgtgccgt tcttgaacgc cacgccgttg ggccggtaga ggccagaggc aagcaccttc
     3661 accgatcgct tgccgtcctt gttgacgacg gcgtagacct tgtcgacgag gcggctgccg
     3721 acgaacaccg tacccttgtc gccttcggcc agtgaccgcg cattcgccat gccggcggcg
     3781 tagacctcga ggttgaagcc ggccgggacc ttaagcttgg cggtcggcag cttgtccggg
     3841 gctgctggaa tcggaggcgg cgccaccggc gcaagttttg cggcggcttc gctggccggc
     3901 cgcccgatga gcggtgaacc cggtggaagc gccggcgcgg cagtcgccgc tggagccgga
     3961 tcggccggct gctgcgcgac cgcaacgcac gcaaatacac caacaaaagc gccagcgagc
     4021 aaaacgctgc gcggcgccga cgagcagatc atcatgatgt cctccccttg agtggaagcc
     4081 ttatgtcctt gcggcacccg cgcgaggtga cttcctaaag cggcactctc cgatattttt
     4141 ccgtcgatgc gcaatcggaa aacatgcagg ggagtcattc ccggatggtc ccaacgggtc
     4201 ggcgctttgc gcggacccgt tggcgtgccc cggaatgacg gggtgtcgca gaataactgc
     4261 ggctaacgtc agtgcttggt gacgggcccg tcggtcatgt cgagcatcgc ttcggcaaag
     4321 ggaaactcca gcacgacctc gccttcatcg tccgtcactt cgaggatcgc gctcaaaagg
     4381 ccacgctcgg cgccttcggt cttcagcagt tcgcggatca tcgcccgcgc gacttcccat
     4441 gcacgatcga gatcccgcag ctcctcgcct tcggggtcgg aaatcaattc gtcgccgatt
     4501 tgcgtgttga aaaagtatcg cggcatgggt caaaaaccac ttccgttaag ggctccaacc
     4561 tgagccgggc gacatctcac aaggtcttta tctcaaactt cgccgcgcaa cgccgagaag
     4621 tctgcattcg gggtggatgg acggcatttt gaagcggttg atccgccttt gttttgacgc
     4681 ccgcccgcac aaataagtga aatccttttg cgttgcagca taaaaatgga gggacccatg
     4741 acaaaagatt tcactggcga acttttctta tccgactagt ttatctgaag ggcggagttc
     4801 gtcccatttt caaggagagc caaaatggcc tggaaaaccc cgaagatcgt cgaagtgccg
     4861 gttggcatgg aaatcaacat gtatgcgtgc gccgcccgca agtaagcggc tgccacgact
     4921 tcacgtttaa cgcaggtgga cctcccggcg tgacacgctc ctgatcggga gcgtgtgtat
     4981 gtcggagtcc accttcgcag tttgaatatg aagcctcgaa tatcgcgaat atcaaggctt
     5041 tggaatctcc aggagacgga tcatgcttcg cgtcgtcgtc ctgggcgccg cggcaggtgg
     5101 tggcgttccg caatggaact gcggatgtcc ggtttgccgg atagctcgaa ccgagcatcc
     5161 cgaacttcaa agcacccagg cttcgatcgc gatcagcgcc gatggcgatc actggttttt
     5221 gatcaacgcg tctcccgatc tgcgtcagca actgatcgcg acgccgcagc tgcaccccaa
     5281 agccggaaga cttcgccata gtccgatatc cggcgtgatc ctgaccaacg gcgaaatcga
     5341 tgcggtcgcg gggctgttgt cgatgcgcga aggctggccg ttcacgatct acgcgcacac
     5401 gcgggtgctt tcgatcctga acagcaacag catcttcaac gtgctgaacg agaagaacgt
     5461 gcggcgccgg ccgatcgagg tgagccagac gttcgagcca accttgccgg atggctcgcc
     5521 gtccggcatc gagatcttgc cgttcgccgt gcccggcaag agcgcgtggt acctggaagg
     5581 caaggatcac cccgcgggag gcgatggcgc gggcgacacg ctcggacttc gcgtcagtga
     5641 cagggcaacc ggcaaatatc tgtattttct ggctgcctgt gctcacgtca cggacgatct
     5701 caagtcccgg cttggcggcg catcgctgat attcttcgat ggcacggtgt ggcgcgatga
     5761 tgagttgatc acggccggcc tcggcaacaa gacagggcaa gggatgggac atattgcaat
     5821 gtcaggcacc actggcgcta tcgcagcctt ggccggactc gatatcgacc ggaagatctt
     5881 tctgcatatc aacaattcca acccggcgct gctgcgcgac tccgcggaac gcgagatagt
     5941 cgagcgcgcc ggctggcaaa ttcccgctga tggaacggag atcacacttt gaacgccatc
     6001 acagcaaaca ccatgacggc cttctcgcgc ggcggcagcc cgcccttgaa cagcgctgag
     6061 gaactggaag cagcgcttcg ccacatcggc gcgacgcgct atcataatct gcatccgttt
     6121 caccggctgc tgcacggcgg caaactgaac aagggacagg tgcaggcctg ggcgctgaac
     6181 cgctattatt accagagcac cattccgatc aaggacgccg tggtgatctc gcgcttccgc
     6241 gaccgcggca tccggcttga atggcggcac cggatcgagg accatgacgg cgatctcggc
     6301 accgaaggcg gcatcgagcg ctggctgaaa ctgaccgaag gcctggggct ggacagcacc
     6361 tacgtcgaat ccaccgaggg catcctgccg gcaacgcgct tcgccgtgga agcctatgtg
     6421 catttcgtcc gcgacaagac accgctcgag gccatcgcct cctcgctgac cgaactgttc
     6481 gcgcccaatc tgcatgaaga gcgtatttcg ggcatgctgg cgcattacga tttcgtcaat
     6541 cctgatatca tgagctattt cagtcgccgc atgcagcaag cgccacgcga cgccaacttc
     6601 gcgctcgact acgtcaagac ccatgcgaac acgcccgaac agcgcgaagc ggtctgcaat
     6661 gcgctgcttt tcaagaccaa tgtgctatgg gttcagctcg acgcgctgta tcatgcctat
     6721 gtcgacggcc acgtgccgcc gggggcgttt gtgcccaaag cgagctaagg ataactaagg
     6781 ccaaaccggc aaaccagaga cgaagcgatg gcaggcctac gcagccgcaa tatcagtgtc
     6841 agcgaggcga gccggccggt tctgccgcgc catgccaagt tgagattcga tgagacgcgg
     6901 cagcgctggg tgattctggc gccggagcgc gtgctggcgc cggacgatgt cgcggtggaa
     6961 gtgcttcaac tctgcgacgg cgtccgcagc gtcgaagcaa tgattgatct gctcgccgaa
     7021 aaatacgccg ctgaccgtgc ggcgattgcc accgacgtca ttgccatgct gcaggacctt
     7081 gccgacaagg gatttctgac cgaagcgcgc gagaaaacgt catgagtcca acgttgacag
     7141 atgccgctgt ggccgccagc gatccctcag acgggctcgc ggtgcttgag cagcgcggct
     7201 cgacggcgga aaccttcggc attccgctcg ccgtgctcgc cgaattgacg catcgctgtc
     7261 cgctgcaatg tccgtattgc tccaacccgg tcgaactgga gcgcggcggc agcgaattga
     7321 ccacggcgga gtggaagaag gtcctcaccg agttggccga aatcggcgtg ctgcagatcc
     7381 atttctccgg cggcgaaccc accgcgcgca aggatctggt cgagcttgtc cagcacgcca
     7441 gcgatgtcgg gttgtactcc aacctcatca cctccgccgt gctgctgacc cgcgaaaggc
     7501 ttgccgcgct ggcggaagcc ggactgtgcc atgtccagat cagttttcag ggcaacgagc
     7561 ctgtggtcgc cgaccgcgtc gccggtttga aaaatgcaca cgagaaaaag atcgaagtcg
     7621 cgaagtggac gcgcgaactg gatttgccgc tgacggtcaa tgcggtgatg catcgccaaa
     7681 acctgcatca gctcccggac attatccaga tggcggtcga tctcgacgcc gaccggctgg
     7741 aggtcgccaa tgtgcagtat tacggctggg ccctgaagaa ccgcgcggcg ctgatgccga
     7801 ccctcgaaca gatcgaggag accagccgca tcgtcgagga agccgaagcc cggctcaagg
     7861 gcattctggc gatcgactat gtggtgccgg attactacgc gctgcggccg aaaaaatgca
     7921 tgggcggctg gggccggcag ttcttcaaca tttcacccgc cggcaagatc ctgccctgtc
     7981 atgccgccga aaccattacc ggactcgaat tcttgtcggt gcgatcaaat cattcgatcg
     8041 cctggatctg gcagaactcc gaggcattta atcgctaccg cggcaccggc tggatgccgg
     8101 agccgtgcaa aagctgcgag ttcagggaaa tcgatttcgg cggctgccgc tgccaggcct
     8161 tcgcgctgac cggcgacgcc gccaataccg atccggcctg cacgctgtcg ccgctgcacg
     8221 agcagatttt caaactggcc gagtccgaag ccgcggctga caacaagcgt ttcatctatc
     8281 gcaattttgc cgggggtacg ctggaggcgg gcggcgatca tggcgcctga cgccgacgcg
     8341 gggaaagcca gtggcaaccg ttccgatccg ttcgcaccgc tgacatcgca gctgctgccg
     8401 gtcggcgacg gccacgagat ctacgtcgaa accgtcggcc gggccgacgg catccccgcg
     8461 gtttatctgc acggcgggcc cggcagcggc tgccagcccg atcaccggcg gctgttcgat
     8521 cccgagcgtt tccacgccgt cttgttcgat cagcgcggcg ccggtcgtag ccgcccaaaa
     8581 ggccgccgcg agcacaatac gctgccgcat ctgatcgcgg acatggagat catccgcgag
     8641 aaatctggtt ttgagcgctg gatcatcgtc ggcggctcct ggggcgcgac gctggctttg
     8701 gcctatgcgc aggcccaccc caaccgggtc accggcatcg tcctgcgcgc gaccttcctc
     8761 ggcacccgcg ctgagctgga gggtgcattt ctcgacgcgc tgccgcgctt ctatcccgat
     8821 ctcagcgatg attttctcaa attgttgccg ccggaggaac gcgcgcagcc gctcgatgct
     8881 tactggcgcc gcattctcga ttccgatccc gcggtgcacg gtccggccgc ccacgcgtgg
     8941 ggcgacaccg aacgaatact ttccggacat gtttcggggc gtgtcaggct cgatcctcat
     9001 tcgccaaact cgacacgcgc cctgcccgcc acgccgttca tggaagcgca ttactttcag
     9061 aacaactgct tcatgcggcc ggggcagctg ctggcggaag cccacaagct ggctggcatc
     9121 cccgcgatca tcgtacaagg ccgctacgat ctgttgtgtc cgccgtccat gtcgcatgcg
     9181 ctggcggcgg tgtgggccgg ttccgaagtc cgctccgtcg agggcgccgg ccatcagctg
     9241 tacgatcccg gcgtccggga tgccgtgatg aaggcgatcg cggatctcac ctccaaaatc
     9301 accagataag gacgtttgca aaatgccatt ggccggtaaa ggaatgctgc tgacatcgat
     9361 ggacatcgat ccatcggacg aagtcgagtt caatcgatgg tacgaccgcg agcatctgct
     9421 cgagcgcgtc gcgatcgacg gctttcttga agcccggcgc tacgtcgcgc atgccggctc
     9481 cccgaaatat ctctgcctct attccaccgc gactttcgag gtgctcgaca gccccaccta
     9541 tcgcaccgtt ctgatgaacc ccaccgactg gtcgaagaca actctcgcgc gtttcaagaa
     9601 catgattcgc gcgatcgcac gtatcacggt gagccgcgga gcgggtcgcg gcgcggcgct
     9661 cggtatcgtc cgcctccgcc ctgccggcga taagggcaag ttgcgcaccg cgctgcggga
     9721 ccggctcgac cccgaaaagc tggacggcat catctccatg catctgatcg agagcgatcc
     9781 ggcactttcc aggccgatca ccgacgatcc cccggtttcc gatccaggtt ctggcgactg
     9841 gttcgtgctg atcgatgcaa cggatgtgcg tgcggtatcg gcggccatca ccgaccgcct
     9901 cggggatgtg ccagcgccga tggccgccga aacgatctcg accggcgtct acaaactgat
     9961 gtgggacttg gcgaagagcg acattcggtc gtcctagatc aaggcacgaa cctcagaaga
    10021 tgatatttgc tgcagtgcca tatttgtccg gtcggcatta aagctggggg cccagaccaa
    10081 tggcgaatgg cgattaagca aaaatggctt ttcagtggat cgtcgatcat attgaatggc
    10141 cggattcatt aagtccatga tatggcagca cacccataac aacgccgtga tgggcgttca
    10201 caacagaaat caaaggccgc gcacgctcgc acccacgcgg acatggtagg aatgaaacca
    10261 actgatatct cggctcccga ttactttcat aaagttgtcg actgccagtg ggcctgtccc
    10321 gcgcatacgc cagttcccga atacatccgg ttgattgctg aaggccgcta cagcgacgcc
    10381 tacatgatca attggagatc gaacgtgttt ccgggaattc tcggacgcac ctgcgatcga
    10441 ccgtgcgagc cggcatgtcg ccgcggccgc gtcgaggaga cgccggtcgc aatctgccgg
    10501 ctgaagcgcg tcgccgccga cttcaaggac gatgtcaaac accgcatgcc gcggccctcg
    10561 ccgaaaaacg gcaagcgtat cgcgatcgtc ggtggcggcc ccgcctcgct gaccgtggcc
    10621 cgcgatctcg cgccgctcgg ctaccactgc accgtgttcg attccgaccc gaaggccggc
    10681 ggcatgatgc ggacgcagat tccgaaattc cggctgccgg attcggtgat cgacgaggaa
    10741 accgattaca tcctcaatct cggcatcgag ttcaagggcg gcacgcgcat cgacagcctg
    10801 aagaagctgc tcggcgaaaa ttttgatgcg atctttgtcg gctccggcgc gccgcgcggc
    10861 cgcgagctca agattcccgg ccgcaaggaa gccgctagcc atattcatat cggcatcgac
    10921 tggctctcca gcgtgtcgtt tggccatgtc gaaaagatcg gcaagcgtgt gatcgtgctc
    10981 ggcggcggca acaccgcgat ggactgctgc cgcaccgcgc gccgtctcgg cggccaggac
    11041 gtcaaggtga tcgtgcgttc cggcttcgag gagatgaagg cgagcccctg ggaaaaggag
    11101 gacgccctgc acgaggacat tccgatcctc aatttcatgg tgccgaaggc gttcacccac
    11161 gacaatggca agctcaccgg cgtcaccttc cagaaggtga aggccgaata tgacgacaag
    11221 ggccggcgca atctggtgcc cgcgggcgac ccggaccaga ccatcgcctg cgacgacgtg
    11281 ctggtggcgg tcggccagga gaacgccttt ccctggatcg agcgcgactg cgg
//
LOCUS       DASWGM010000282         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1265939,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000282 DASWGM010000000
VERSION     DASWGM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265939"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VGA15_08240"
     CDS             <1..822
                     /locus_tag="VGA15_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9587720.1"
                     /translation="FMAGVVPGMMIGFGLMIYCYFFGPSGMRKSRAPLRQVVFAAGDA
                     ALPLMIPVILLGGILTGWFTPTEAGVVAVIWIIAVVIPALNRGHLRKIPYDFCLAGLI
                     FSLPLITIGAANAFGWMLAYLRGAIVIADWITSIAGSDPHIMMLLLVLLFTVVGDFIE
                     PVPTIIIFMPLVNALTQAGDINGVHMGVVLIATLAFGLITPPYGLVLLMASKFVGVSF
                     GKALRAALPIYVVFLVTICFTIYFPKVVLWLPKQVIPESVGCFKSPAGTGYICPN"
     gene            complement(1000..1806)
                     /locus_tag="VGA15_08245"
     CDS             complement(1000..1806)
                     /locus_tag="VGA15_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9587721.1"
                     /translation="MPTPDHATVSLPDDPALLRIEGPIATITLNRPAAFNSIDLSIAK
                     KLEQLGAEVESSDDIRVLVIEGVGRAFCAGGDLQTIGAAAAADTIAPVVGELLKHYHA
                     FIETVRRMPKIVLSSVHGSAAGAGMGLAFVTDLCIAADDARFTPAYAKIGVSPDGGST
                     VGMVGTVGIRRALQIFLAEDSFSAQQAYDWGLVAKIVPAAELKAATQTFAERLAQNPS
                     AAIGGTKSLVYQAAVTPTKQQLDAEEEKIIDCMHTDEFRVAVKKFTSKAK"
     gene            1956..3158
                     /locus_tag="VGA15_08250"
     CDS             1956..3158
                     /locus_tag="VGA15_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9587722.1"
                     /translation="MSIDTTVDRPVSAIDPFSHEFLRDPYPHHEALREAGPVVWLEQY
                     GIWTMARHQEVRDALTDWQTYCSSAGVGLSDFRKEPPWRPPSIILEADPPLHTRTRAV
                     LTRILSPSAINILRATFEREAELLVARLVEQREFDGVRDLAEAYPLKVFPNAVGISED
                     GRENLLPYGSMVFNSFGPRNDLFDLAMANAGPVRDWIMSKCSRAALAPGGLGMQIFGA
                     VDAGELTEAEADMLVRSFLSAGIDTTVYGLGNALHCFARYPEQWAVVRENPQLIRGAF
                     EEVLRFEAPVQTFFRTTTRDVEVGGVRLGDGEKVLLFLAAANRDPRRWENAEKFDVRR
                     RATGHMTFGTGIHGCVGQAVARLESEAIFGALARRVASFELTGEPTRRLNNTLRGLDT
                     LPLRIVPA"
     gene            3163..>3755
                     /locus_tag="VGA15_08255"
     CDS             3163..>3755
                     /locus_tag="VGA15_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9587723.1"
                     /translation="MTQGTVLIVGAGHAGFQLAASLRQHGFGGRIGLINDEAHLPYQR
                     PPLSKAYLKGAGGADSLMFRPDKFYRDQNIELIADRAVSIDRGARRLLLASGASLDFG
                     HLVLATGARNRLLDIPNANLADVRYLRILDESEALRRRITSGQRVVVVGAGFIGLEFA
                     ATARAKGLEVDVVELGARVMARAVTAEISDYFQERHTA"
BASE COUNT          638 a         1158 c         1221 g          738 t
ORIGIN      
        1 ttcatggccg gcgtggtgcc gggaatgatg atcggctttg gtctcatgat ctactgctat
       61 ttcttcgggc cctccggcat gcgcaagtcg cgggcgccgt tgcgacaggt cgtgttcgcc
      121 gccggcgatg ccgcgttgcc gctgatgatc ccggtgatcc tgctcggcgg cattttgacc
      181 ggctggttca cgccgacgga ggccggcgtc gtcgcggtga tctggatcat cgcggtggtg
      241 atccccgcgc tcaatcgcgg gcatcttcga aaaattccct acgatttctg cctggccggg
      301 ctgatcttct cgctgccgct gatcacgatc ggcgccgcca atgcgttcgg ctggatgctg
      361 gcctatctgc gcggcgccat cgtcatcgcc gactggatca cctcgatcgc cggcagcgat
      421 ccgcatatca tgatgctgct actggtgctg ctgttcaccg tggtcggcga tttcatcgag
      481 ccggtgccga ccatcatcat cttcatgccg ctggtcaatg cgctgacgca agccggcgac
      541 atcaacggcg tgcatatggg cgtggtgctg atcgccacac tggcattcgg actgatcacg
      601 ccgccctacg ggctggtgct gctgatggcg tcgaaattcg tcggcgtcag tttcggcaag
      661 gcgctgcgcg cggcgctgcc gatctacgtc gtattcctgg tgacgatctg cttcacgatc
      721 tatttcccga aggtggtgct gtggctgccg aagcaggtga tcccggaatc ggtcggctgc
      781 ttcaagtcgc cggcggggac ggggtatatt tgtccgaatt gaaaatcccc ggtggtcgtc
      841 ccggcgaacg ccgggaccca taacccctgg cgttagtttt tgacaaaagg cgtccacctc
      901 attgctaaaa cgagacggcg cggcgccgcg atgagcgcaa aagcgctcac gctagggatc
      961 ccggcgttcg ccgggacgac tcgtgaagat gtctcaacct tacttcgcct tgctcgtgaa
     1021 cttctttacc gcgactcgga attcgtcggt gtgcatgcaa tcgatgatct tctcttcttc
     1081 ggcgtcgagt tgctgcttgg tcggggtgac cgcggcctgg tagaccagcg acttggtgcc
     1141 accgatcgca gccgacgggt tctgcgccag tcgctcggcg aatgtttgcg tcgccgcctt
     1201 caattccgcc gccggcacaa tttttgcgac cagaccccaa tcgtacgcct gctgcgcgga
     1261 gaagctgtct tcggcaagaa atatctgcaa cgcgcggcgg ataccgacgg tgcccaccat
     1321 gccgacggtg ctgccgccgt ccggcgacac gccgatcttg gcgtaggccg gcgtgaagcg
     1381 agcatcatcg gcggcgatgc agagatcagt gacgaaggcc agtcccatgc ccgcgccggc
     1441 cgcggagccg tgaacgctgg acagcacgat cttcggcatc cgcctgacgg tctcgataaa
     1501 cgcgtggtag tgcttgagca actcgccgac caccggcgcg atcgtatcgg cagcggctgc
     1561 agcaccgatg gtttgcagat cgccgccggc gcagaatgca cggcctacgc cttcgatcac
     1621 gagcacccgg atatcgtcgg atgactcgac ctcggcgccg agctgttcga gtttctttgc
     1681 gatcgaaaga tcgatcgaat tgaacgcggc cggccgattg agcgtgatgg tggcgatcgg
     1741 gccttcgatc cgcaacagcg ctggatcgtc gggaagggaa actgtggcgt gatcaggtgt
     1801 cggcatttga aaaaggcctt ggatcgaaag gatcgcaggc atttaaatag cagaagggaa
     1861 cgggtgacaa tccccggcca agtcggcaag atgcggacaa acggccaggc gggatcaccg
     1921 catcgcaaca acagccagag gcgggagcag gcgccgtgag tatcgatacg acagtcgacc
     1981 gtccggtctc cgccatcgat ccgttttcgc atgaattcct gcgcgatccc tatccgcacc
     2041 atgaggcgct gcgcgaggcc ggccccgtgg tctggctcga gcaatacggg atatggacca
     2101 tggcccggca tcaggaggtc cgcgacgcgc tgaccgactg gcagacctat tgctcgtcgg
     2161 cgggcgtcgg cttgagcgat ttccgcaaag aaccgccatg gcgcccgcca agcatcattc
     2221 tggaggccga tccgccgctg cacacgcgca cccgcgccgt gctgacacgg atattgtcgc
     2281 catccgcgat caatattctg cgcgccacgt tcgagcgcga ggccgaactg ttggtcgcgc
     2341 gcctcgtcga gcagcgcgaa ttcgacggcg tcagggatct cgccgaggcc tatccgctaa
     2401 aagtgtttcc gaacgctgtc ggtatttccg aggacggccg cgagaacctg ctgccctatg
     2461 gcagcatggt gttcaactcg ttcggcccgc gcaacgacct gttcgatttg gcgatggcca
     2521 atgccggtcc ggttcgcgac tggatcatgt cgaaatgcag ccgcgcggcg ctggcgccgg
     2581 gcgggctcgg catgcagatt ttcggggccg tcgacgccgg cgaactgacg gaggctgagg
     2641 cggacatgct ggtgcgctcg tttctttcgg ccggtattga tactaccgtc tacgggctcg
     2701 gcaacgcgct gcattgtttt gccaggtatc cggagcaatg ggctgttgtt cgcgagaatc
     2761 ctcaactgat ccgcggcgcc ttcgaagagg tattgcgctt cgaggcgccg gtgcagacgt
     2821 tcttccggac cacgaccagg gatgtcgaag tcggcggggt gcggttaggc gacggtgaga
     2881 aggtcttgct gttcctcgcc gccgccaacc gcgacccgcg ccgctgggaa aatgcggaaa
     2941 agttcgacgt cagacgccgc gccaccgggc acatgacctt cggcaccggc attcatggct
     3001 gcgtcgggca ggcggtggcg cggcttgaaa gcgaggcgat tttcggcgca ctcgccaggc
     3061 gcgtcgcgtc gtttgaactg acgggcgaac cgacgcggcg gctcaacaac acgctgcgcg
     3121 ggcttgacac cttgccgctg cgcatcgtac ccgcctgatg gtatgacgca gggaacagtt
     3181 ctgattgtcg gcgccggcca cgcgggtttt cagctcgcgg catcgttgcg gcagcatggg
     3241 tttggcgggc gcattggcct gatcaatgat gaggcacacc tgccgtatca gcggccgcct
     3301 ttgtcgaaag cctatctgaa aggcgcgggc ggggccgaca gcctgatgtt ccggccggac
     3361 aaattttatc gcgaccagaa catcgagctg atcgcggatc gcgccgtctc gatcgaccgc
     3421 ggcgcgcgcc ggctgttgct ggcgtccggc gcgtcgctcg actttgggca tctggtgctg
     3481 gccaccggcg cgcgcaatcg cctgctcgac atccccaacg ccaatcttgc ggacgtgcgg
     3541 tatctgcgaa ttctcgacga gagcgaagcg ctgcggcggc ggatcacctc cggacagcgt
     3601 gtcgtggtcg tcggcgcagg attcatcggc ctggaattcg ccgccaccgc ccgggccaag
     3661 ggcctcgagg tggatgtggt cgagctcgga gcccgcgtga tggcgcgcgc ggtgaccgcg
     3721 gaaatctcgg actactttca ggagcgccac accgc
//
LOCUS       DASWGM010000283        17806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1404569,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000283 DASWGM010000000
VERSION     DASWGM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17806
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404569"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..489
                     /gene="rplM"
                     /locus_tag="VGA15_08260"
     CDS             25..489
                     /gene="rplM"
                     /locus_tag="VGA15_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX9587724.1"
                     /translation="MKTFSAKPAEVTKKWVLIDAKGLVVGRLASLVAMRLRGKHLPIY
                     TPHVDCGDHVIIINAAHVVLTGRKRDNKVYYNHTGFIGGIKERTAKSILEGRFPERVV
                     EKAIERMIPRGPLGRVQLGNLRVYPGPEHPHEAQQPETVDVASMNRKNMRAA"
     gene            491..967
                     /gene="rpsI"
                     /locus_tag="VGA15_08265"
     CDS             491..967
                     /gene="rpsI"
                     /locus_tag="VGA15_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX9587725.1"
                     /translation="MSDTMQSLDQLSQLKPATPEAPKYVKKVDKLGRAYATGKRKDAV
                     ARVWIKPGAGKVTVNTREVEVYFARPVLRMMIQQPLVAAARAGQYDVVCTVAGGGLSG
                     QAGAVRHGISKALTYFEPDLRGVLKKGGFLTRDSRVVERKKYGRAKARKSFQFSKR"
     gene            1197..2423
                     /locus_tag="VGA15_08270"
     CDS             1197..2423
                     /locus_tag="VGA15_08270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9587726.1"
                     /translation="MSLDTTTLYFVATMVAALLGAMLLFFGKQEKIPALNWWGTAYLL
                     GAASIALWTLAGSTLGAMLSLALNALGFVACGMVWNAARVFHGRKPNLPGLMLGAVAW
                     VAAVMALRPEASAIRLTIGAGIVAIYAALTAAELWSERRKTMQKRWPAIAVPVLHGLV
                     LMLPILLGGLLHPHDNAFASSVWVTVFAIELVLYAVGTVFVIFMLVSERTVSAHKTAA
                     SMDPLTGLFNRRGFAEATSRVIEREANAGRPVTVMIFDLDHFKSINDRFGHPAGDEIL
                     KLFATVVINTLRITDLSGRIGGEEFAALLPCPLEEGVIAAERVRETFAASGIVVQEGP
                     VETSVSIGVAGGPAGTELEVLLAAADSALYQAKRNGRNRVEAAEELPLSLEKWRRKSA
                     GLPGHLQQQPGVARPA"
     gene            2481..2717
                     /locus_tag="VGA15_08275"
     CDS             2481..2717
                     /locus_tag="VGA15_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587727.1"
                     /translation="MYRPRSQSPHAALTSLEAAAVAARGGFACMFSTSDEYETALILE
                     RRAQGRYDRRRSPWPAVMFVGCALMAAGTVFLLH"
     gene            2841..3143
                     /locus_tag="VGA15_08280"
     CDS             2841..3143
                     /locus_tag="VGA15_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX9587728.1"
                     /translation="MITEIAQIDVKPGTEKDFEAAVAKARPLFLRAKGGKGFELHKSI
                     EKPSRYRLMAKWDTLENHTVDFRGSEDFTAWRGLVGQYFASPPEVEHTETVLTSAG"
     gene            complement(3164..4291)
                     /locus_tag="VGA15_08285"
     CDS             complement(3164..4291)
                     /locus_tag="VGA15_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9587729.1"
                     /translation="MTISADHRPAARNDLAWAISVGGIGVVAFAALLVFAWHFAATLF
                     LIFAGMLLGVALNAMTNMLGRWVRLPHSLRLTIVCLVLAGLLSGVVFLGGSTIVQQAT
                     ALSGTIKSQLVGVKAFLERNGVDTSYFELGNPAATATDSSTPATSGAPATHNLPSAGA
                     IASGGGAIVSQTLKLLLGTVSAVGNFFIVLFLGLTFAAQPSVYRKGLLFMAPVRHRAR
                     ATAIVDRIGETLERWLIAQIITMFAVFLVTWIGLSIIGIQSSFILGIQAGLLAFIPTV
                     GALLGGLIVVLASLASGWVAAASAFALFLGIHALESYILTPIIQRQALDIPPATLFAF
                     QILLGVVFGIWGLALALPLMAIVKVMIDHFKANEPPEAVAA"
     gene            4442..5140
                     /locus_tag="VGA15_08290"
     CDS             4442..5140
                     /locus_tag="VGA15_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587730.1"
                     /translation="MNFKWVGPLALLAALVIGDQIRINRPGHKYRLTVEVETPEGGKS
                     SSGVLAVHPDRSYSRGGHTLTRGDAVLVDLGGGKNLLALLAHIDNKSVDLDGMNYVAL
                     RAYNAAGHKASFNEMSRMTGAVPVKGALVPVLVTFTDLNDPGTAREVLPDEAEAGLEK
                     GFHVSSISVEVVPNGLWPLDFGGPLGEPVTRGIEAKLPWWNAADNPAARALQAAGLQT
                     GDAIDPKEVFTRKQ"
     gene            complement(5149..6570)
                     /locus_tag="VGA15_08295"
     CDS             complement(5149..6570)
                     /locus_tag="VGA15_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9587731.1"
                     /translation="MGLLNRLGLHRPGPPRCSAEGRFQIAGVTVINPMHDRRARATLD
                     ISSGAIAGISDTHAVPPSELAGCFALPGLVDMHVHLPPDNALKLTQGAALLYLLHGVT
                     SIREAGDLDGTAVAAARRLSSEAAHPVPRVFSCGPFVGAGRATFKNTILLKDASPAAA
                     DAAACRVKADGASFMKFYEGLTEPMIRALEQACAKHGLKMMGHVPAGITYEDARIAEV
                     QHFFGVPEPQTLERDTLVNRSCDWHAVDERRMDRIVEATLEHGIANTPTIVTNQKMLC
                     YRDFEAARRQSDMSKVPPFYLDVIWHPERGRALNRMQRDYLERQVVPAIAKKQQLTRK
                     LFDAGAQLYLGTDVAQPFLIPGASLLEEMALFVEAGIGVEQVWKLATSDAGDRLGLRG
                     LGRVETDAPADILLFRRDPTEAIGNISSLEAVIAAGKLYRVANLNRALQSSQAYFTSP
                     LIKPLARRGAERALARTFSRNQG"
     gene            6681..7325
                     /locus_tag="VGA15_08300"
     CDS             6681..7325
                     /locus_tag="VGA15_08300"
                     /inference="COORDINATES: protein motif:HMM:NF037005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9587732.1"
                     /translation="MAKPASDKRTGPRKPPTQARSRARVENILKVAGALLGEVGYDGL
                     STNLIAERADVPVGSIYQFFQGKDDIVAALVGQFQDRILRLAGEQLDAASAIQDRRAF
                     IAKLVDGIAGIQAEASAFVCVFSGSQSHARFDGLARDLRRTLTRHLDRVFAEAFPRLP
                     KDDRGRMLAAWSDITGAIIANLDRSKPGERGKLLEELKTVLAAYLDAKLAASPA"
     gene            7453..8223
                     /locus_tag="VGA15_08305"
     CDS             7453..8223
                     /locus_tag="VGA15_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX9587733.1"
                     /translation="MRRPVVGVIGNAYRIEDRFATQLVGERNLRAVAEVSGALPLMFA
                     GSPDITDIGALLDAVDGVVLTGARANVHPTRFRTEPDPRHEPYDLHRDDLALALSEAC
                     VDRGIPLFGICRGLQEMNVAFGGSLHPEIRDLPGRINHRMPRLENGEIHPDPNVIFAD
                     RHEVSLVPGGAFATLLGCETIRVNSLHGQGILEPGKRVVVEGVAEDGTIEAIRIADAT
                     GFALGVQWHAEYDPQRNPINRALFQAFGDALLVRKHRS"
     gene            8533..11712
                     /locus_tag="VGA15_08310"
     CDS             8533..11712
                     /locus_tag="VGA15_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9587734.1"
                     /translation="MSLLLGRQAQNIEHKPKQAVSGIKLSTRLTLAMVSLVVVTTSVL
                     SFITYHSITQAAVPRALDRLATKAVLCASKLESALNVARQDVMVVQGSTGVIQMGVAR
                     AANPLKPVSDLPLRESIAARLLAALAAKPEYSQLRVIGVANGGRELLRVDRRGPGGAP
                     RIVPEAELVQTGERDYFKRAIGQSKSDVFVSPIELQKEGATEGPAVPLLHVGVPLLTP
                     SGQPWGISVIDFDIGPKFDRIRVEGGRENQVFVTSATGDYLLHPDRSREFAFETGAAV
                     RIQHDFPGFDEAIAGGAANDSGIWQDRSGVRFGVGWARVPLAGGAGLTILVAATYSNL
                     TVGLAAVNSSALIGGAVAILLAICLAIAIARSLSKPLVQITRAAEGLSRGELMAMPSD
                     GGREISALSATFAEMATQIRTKQSLLENTIESIGDSVLVADERGQIVVANAAAKRLLQ
                     IVPGAGVRGKFSYFYPDGVTPMPASRLALVRALGGESVDDQELFVVPEAPCVPAYVVA
                     NARPLRDETGAIRGALTVLRNITEHKRAHQSLVDSQQMAQAIINTALDAFVQTDEAGV
                     ILDWSPHAEAMLGWTRSEAIGAKAEDLIVPELQRDSNNQWVKQFLHDVGIGAKGWRFE
                     APLLHRDGSEIFTEMSLTELRRGEGHIINAFIRDITQKRAAEEQLIQAQKMESVGQLT
                     GGIAHDFNNMLTVITGTIEILAEAVRNQPHLARIVTLISEAADRGSELTANLLAFARK
                     QPLQPVEIDVNALVNEVGRLLSPTLGRQIEIKTALGGDVWPALVDPGQLSSALVNLAI
                     NARDAMPDGGTLTFATSNVTLDGRDAAASGIDRVDDYVVVEVTDTGTGIPEAIRDKIF
                     DPFFSTKETGQGTGLGLSMVFGFAKQSGGNIEVDSEEGHGTTFRIYLPKADFEASQLP
                     SADDLQSTGGTETILCVEDDASVRAYVIAQLESLGYKAIAASNAAEALAIADGGAEFD
                     LLFTDVVMPGRMNGKQLAERMRLRRPSLRVLFTSGYTDHTIIRDGRIMRDVFFLSKPY
                     RRPQLARMVRRSLDAPLVAFVVPSQPKSA"
     gene            11751..12938
                     /locus_tag="VGA15_08315"
     CDS             11751..12938
                     /locus_tag="VGA15_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9587735.1"
                     /translation="MPSRQKTALPEQVEFIDICYNASANIAAVREWSRNHGEDAVQDR
                     KWSRRKVLKVSAVSAAATLFAEPLVAAAPPATEVTSALVEAARKEGKVSFYTALELNV
                     AERLSRMFEAKYPGIAVRVERSGAERIFQRIAQEQASGINAVDVANSTDPAHYPDRKN
                     HGWLAPYVPDDVARHFPADQIDADGMHATSCAWTEAIGYNTSLVKQEDAPKSYADLLD
                     PKWQGRIVKGHPGYSGAIMTATFVLARDLGWSYLEKLAQQKVMQVQSAADPPKKILLG
                     ERAIMADGNDYNLVLLKDQGKPVEVVYPTEGSPLIIVPSGIFQGAPNPNAARLFQSFF
                     FSAEGQQLLVDGFAHRSFHALIKEKAGHTPLSTLKLLKADPAAVQAQSEEIKARYAKV
                     FGV"
     gene            13177..14250
                     /locus_tag="VGA15_08320"
     CDS             13177..14250
                     /locus_tag="VGA15_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9587736.1"
                     /translation="MSASFALAAAAGGFSCIETASAGPIDVPTIDKLSVRVLVDSASD
                     IFFRPQEANGVKTEPGRSADSTRPLHSEWGLSLLLEPQRGDDKRTFLLDYGWTPEVIN
                     GNMDLLKVDASKIDTLIMSHGHFDHWGGLLGFLDKHRKDLPADLTMYAGGEDNFCQRY
                     ARAGQGELSDFGMLDRRDIARQNVKLVLCESPVVIGGQAFTTGKIKRSSIEKVLPNTM
                     VEFKLKDGAGCNASHYLPAEMEGKIVPDEHIHEHATCFNLKDKGLIVISSCGHVGIVN
                     SVRQAMEVSGVSKIHAIMGGFHLGPAPADYLTQVVSEIGKFNPDVLIPMHCSGLNFTQ
                     EAQRQMPGKVLTTTTGTRISFGT"
     gene            14373..14867
                     /locus_tag="VGA15_08325"
     CDS             14373..14867
                     /locus_tag="VGA15_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9587737.1"
                     /translation="MYGRGSVGGPFTLTDQAGKQRSDTDFRGKLMIVYFGYTYCPDVC
                     PTDLMAITQALDALGSAAEGVQPIFITIDPERDTNLLADYVSAFHHSFVGLTGPAEEI
                     RKVANSYKAFYVKVPDERSGNYSIDHAGVIYLMGRNGEYLGFMPPRTNPDRLTEVLRK
                     NLAN"
     gene            complement(15089..16498)
                     /gene="glnA"
                     /locus_tag="VGA15_08330"
     CDS             complement(15089..16498)
                     /gene="glnA"
                     /locus_tag="VGA15_08330"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX9587738.1"
                     /translation="MKTAKDVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDIGMIEEDT
                     FAEGLMFDGSSIAGWKAINESDMCLMPDPVTATIDPFFAETTMVITCDVLEPTTGEPY
                     NRDPRGMAKKAEAMVKSMGVGDTVYFGPEAEFFVFDDVRFQTTPYNTGFKLDSSELPI
                     NSDTEYEGGNLGHRIRTKAGYFPVPPQDSVQDMRSEMLGAMAKMGVKVEKHHHEVASA
                     QHELGMKFDTLTLMADQMQIYKYCIHQVAHIYGKTATFMPKPVYGDNGSGMHCHQSIW
                     KEGKPVFAGNKYADLSETCLFYIAGVIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLA
                     YSARNRSAACRIPYTSNPKAKRVEVRFPDPMANPYLGFAAMLMAGLDGIKNKLDPGPA
                     MDKDLYDLPKEELKTIPTVCGSLREALECLDKDRAFLKNGGVFDDDFIDSYIELKMTE
                     VERFEMTPHPVEFEMYYSL"
     gene            complement(16650..16988)
                     /locus_tag="VGA15_08335"
     CDS             complement(16650..16988)
                     /locus_tag="VGA15_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9587739.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHAE
                     LYRGAEYIVDFLPKVKIEIVIGDDLVERAIDAIRRAAQTGRIGDGKIFVSNIEEAIRI
                     RTGESGLDAI"
     gene            17367..>17806
                     /locus_tag="VGA15_08340"
     CDS             17367..>17806
                     /locus_tag="VGA15_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX9587740.1"
                     /translation="MEVLDTAEMERADRLTIAAGTPGFALMLSAGQAVAEAAIDLVAE
                     GPMLVVAGSGNNGGDGFVAAAELAARGREVSVILLCERDSLRGDAASAARGWKYPVLP
                     CNPQAIGKPALIIDALFGAGLNRPVKGDPYDMIEAINANGAPVL"
BASE COUNT         3499 a         5227 c         5623 g         3457 t
ORIGIN      
        1 ttcccctttc acggatttcc tcacatgaaa acgttttcgg caaagcctgc cgaggtgacg
       61 aagaagtggg tgctgatcga cgccaaaggt ctggtggtcg gacggctcgc ctcactggtc
      121 gcgatgcgcc ttcgcggcaa acacctcccg atctatacgc cccatgtcga ttgcggtgac
      181 cacgtcatca tcatcaacgc ggctcacgtg gtgctcaccg ggcgcaagcg cgacaacaag
      241 gtctattaca atcacaccgg cttcatcggc ggcatcaagg aacgcacggc gaagtcgatc
      301 ctcgagggcc gtttccccga gcgcgtggtc gagaaggcca tcgagcgcat gattccgcgc
      361 ggaccgcttg gccgggtgca gctcggcaac ttgcgcgttt accccgggcc cgagcatccg
      421 cacgaggccc agcaacccga gacggtggac gtcgcttcga tgaatcgcaa gaacatgagg
      481 gccgcataag atgtccgaca ccatgcagtc gctcgatcag ttgtcgcagc tgaagccggc
      541 cacgccggaa gcgccgaaat acgtcaagaa ggtcgacaag ctcggccgcg cctacgccac
      601 cggcaagcgc aaggacgcgg tggcccgcgt ctggatcaag ccgggcgccg gcaaggtcac
      661 cgtcaatacc cgtgaggtcg aagtctactt tgcacggccg gtgctgcgga tgatgatcca
      721 gcagccgttg gtcgcggcag cccgcgcggg gcagtacgac gtggtctgca ccgtggccgg
      781 tggcggtctg tcgggccagg ccggcgcggt gcgtcacggc atctcgaagg cgctgaccta
      841 tttcgaaccg gatctgcgcg gcgttctgaa aaagggcggc tttcttaccc gcgactcgcg
      901 cgtggtcgag cgcaagaagt acggccgcgc caaggcgcgc aagtcgttcc agttctcgaa
      961 gcggtaaccg caccggtaaa attagctgcg aatccagcag agatgaaact tcaaaaggcg
     1021 ccttcgggcg cctttttttg atcatttgcc gaagagttga ggcagttttt ccggaaaact
     1081 tgcacgtgca aacaaaggga tttcaaacgc ggtcgacgta gatctaaaaa gaccaaggcg
     1141 cgtgctctct ttcaagcgcg ccggatacgc tgcattgcaa tcttattcgg tggtccatgt
     1201 cgctcgatac cacgacgctt tatttcgttg ccacgatggt cgcggcactg ctgggagcca
     1261 tgctgctgtt tttcggaaaa caggagaaga tccctgcact gaactggtgg ggaaccgcct
     1321 atctgctcgg cgccgcatcg atcgcgctct ggacacttgc cggttccacg cttggcgcga
     1381 tgctttcgct ggcgctgaat gcgctcggtt tcgtggcctg cggcatggtc tggaatgcag
     1441 cgcgcgtgtt ccacggccgc aagcccaatc tgccgggact aatgctcggg gccgtggcct
     1501 gggtcgctgc tgtcatggcg ctgcggccgg aggcgtcggc gatacgcctg acgatcggcg
     1561 ccggcatcgt tgcgatatat gcggcgctga cggctgccga attgtggtcc gagcgtcgca
     1621 agacaatgca gaagcgctgg ccggcgatcg cagtgccggt gctgcacggt ctcgtgctga
     1681 tgctgccgat cctgcttggc ggcctgctgc acccgcatga caacgcgttc gcgagcagcg
     1741 tctgggtgac cgtgttcgcg atcgagcttg tgctctatgc ggtcggcacc gtgttcgtga
     1801 tcttcatgct ggtgtcggag cgcacggtga gcgcgcacaa gacggcggcg tcgatggatc
     1861 cgctgaccgg cctgttcaat cgccgcggct ttgcggaggc gacgtcgcgg gtgatcgagc
     1921 gcgaagccaa cgccggccgc ccggtgacgg tgatgatctt cgaccttgac catttcaagt
     1981 cgatcaacga ccgcttcggc cacccggcgg gcgacgagat cctgaaactg tttgccacgg
     2041 tggtgatcaa cacactgcgg atcaccgacc tgtcgggacg aatcggtggc gaggagttcg
     2101 ccgcattgct gccctgtccg ctggaggaag gtgtgatcgc cgccgaacgc gtgcgggaaa
     2161 cgttcgcagc ttccggaatc gtggtccagg agggaccggt cgaaacgtcg gtcagcatcg
     2221 gcgtggccgg tggcccggcc ggcaccgagc tcgaggtgct gctcgcggcc gccgacagcg
     2281 cgctttatca ggccaagcga aatggccgca accgcgtcga ggcagcggaa gagctgccgc
     2341 tgtcgctgga aaaatggcgg cgaaaatctg cgggccttcc gggtcacctg caacagcagc
     2401 cgggtgtcgc ccgtccggcg tagggcggtg gcgcgcggcg ataacctccc gtttaccatt
     2461 cggttcatat cattggggtc atgtacaggc cccgcagcca aagccctcac gcggctctga
     2521 cctcgcttga agccgcggcc gtagcggcgc gcggcgggtt tgcctgcatg ttctcgacgt
     2581 cggacgaata tgagacggcg ctgattctgg aacggcgcgc gcagggccgc tatgatcggc
     2641 ggcgaagccc ttggccagcc gtcatgttcg tcggctgcgc gctgatggcg gcaggcacgg
     2701 tgtttctttt gcactgagga gcctcgggaa gtggggcggg tgccgcgagg caccgtttta
     2761 gttttgaacg ttccgtgact tcaataatgc ggccggctgg gttagggatg tgaattctca
     2821 acgggaggcc gagaacccaa atgatcaccg aaatcgcaca aatcgacgtc aagcccggca
     2881 ccgagaaaga ctttgaggcc gcggtcgcca aggctcgccc gctgttcctg cgcgccaagg
     2941 gcggcaaggg ttttgagctg cacaagtcga tcgagaagcc gtcacgctac cggctgatgg
     3001 caaaatggga cacgctggaa aatcacaccg tcgatttcag gggttcggag gatttcacgg
     3061 cgtggcgcgg gctggtcggg cagtattttg cctcgccacc ggaagtcgaa cacaccgaga
     3121 cggtgctgac ctcggcaggc tgattaaccg atgacgggcg gcctcaggcc gccaccgctt
     3181 cgggcggttc gttggccttg aaatgatcga tcatgacttt cacgatcgcc atcaacggca
     3241 aagccagcgc caaaccccag atgccgaaca cgacgccgag caggatctgg aacgcgaaca
     3301 gcgtcgctgg gggaatgtcg agcgcctgac gctggatgat cggcgtgaga atgtagcttt
     3361 ccagcgcgtg aatgccgagg aacagtgcga atgcgctggc cgcggcgacc cacccggatg
     3421 cgaggctcgc gagcaccacg atcaacccgc cgagcagcgc gccgaccgtc ggaatgaagg
     3481 cgagcagtcc ggcctggatg cccagaatga aggaactctg gatcccgatg atcgagagac
     3541 cgatccaggt taccaggaaa accgcgaaca tggtgatgat ctgcgcaatc agccatcgct
     3601 ccaacgtttc gccgatgcga tcgacgatcg cggtcgcgcg tgcgcggtgc cgaactggcg
     3661 ccatgaacaa cagccccttt cggtagacgc tcggctgcgc cgcgaacgtt agcccgagaa
     3721 acagcacaat aaagaaattt ccaacggcgc tcaccgtacc cagaagcaat ttcagggtct
     3781 ggctcacgat cgcgcccccg cctgaagcga tcgcgccggc gctgggaaga ttatgtgtcg
     3841 ccggcgcacc tgatgttgcc ggtgtcgatg agtccgtagc ggtcgcggcc ggattgccga
     3901 gctcgaaata gctggtatcg accccgtttc tctcgaggaa cgctttcacg ccgaccagtt
     3961 gcgacttgat cgtgccgctc agcgccgtgg cctgctgcac gatggtggag ccaccgagaa
     4021 agaccacgcc cgacagcagg ccggcgagca ccagacagac aatcgtcagc cgcagcgaat
     4081 gcggcagtct gacccagcgg ccgagcatgt tggtcatggc gttgagcgcg acaccgagca
     4141 gcattccggc gaagatcaaa aacagcgtcg cggcgaaatg ccaggcgaaa accaacaaag
     4201 cggcaaatgc gacgacgccg atgccgccaa ccgatatcgc ccatgccaga tcgttgcggg
     4261 ccgcagggcg atggtcagcc gagatggtca cattgattcc ttgtcaattt aattctccac
     4321 agcactcttt cgtcaaatgc gcccgggatc aagcggagaa ggcgcgccgg tttgacgctg
     4381 gcatgttggt tgtgcgaagc gatcaatgaa tgggatcgcg gcgtggcgag aggctgctag
     4441 catgaatttc aaatgggtcg ggccgcttgc tttattggcg gcgcttgtga tcggcgacca
     4501 gattcgcatc aaccggcccg gtcataaata ccgtctgacg gttgaggtgg aaacgcccga
     4561 agggggcaaa tcgtcctccg gggtcttggc ggtccatccc gacagaagct acagccgtgg
     4621 cgggcacacc ctcaccaggg gcgatgccgt attggtcgat ctcggcggcg ggaaaaacct
     4681 cctcgcattg ctggcgcaca tcgacaacaa atcggtcgat ctcgacggca tgaactatgt
     4741 ggcgttgcgc gcctacaacg cggccggcca caaggcgtca ttcaacgaga tgagccggat
     4801 gaccggcgcg gtgcctgtga aaggcgcgct ggtcccggtt ctcgtcactt ttacggatct
     4861 gaacgatccc gggaccgcgc gcgaggtgct gccggatgaa gcggaggcgg ggttggagaa
     4921 aggctttcac gtcagctcga tttcggtaga ggtggtgccg aacggtttgt ggccgctcga
     4981 cttcggcggt ccgctcggcg agccggtgac acgcgggatc gaggcgaaat tgccgtggtg
     5041 gaatgctgcg gacaatccgg cggcgagggc acttcaggca gcgggattgc agaccggcga
     5101 tgcgattgat ccaaaggagg tcttcacgcg aaaacaatga ccgcgaaatt acccctggtt
     5161 gcggctgaat gtgcgcgcca gcgcacgctc ggcgccgcgc cgcgcgagcg gcttgatcag
     5221 cggcgaggtg aaatacgcct ggctcgattg gagcgcgcga ttgagattgg cgacgcgata
     5281 tagtttgccc gccgcgatca cggcctccag gctcgaaatg ttgccgatgg cttcggtcgg
     5341 atcgcgccga aacaatagga tgtcggccgg agcgtcggtt tcgacgcggc caaggccgcg
     5401 caggccgagc cggtcgcccg catctgatgt cgcgagcttc cagacttgtt cgacgccgat
     5461 cccggcctcg acaaacagcg ccatttcctc aagcaggctg gcgccgggga tgaggaacgg
     5521 ttgcgcgaca tcggttccga gatacagctg cgcgccggca tcgaagagtt ttctcgtcag
     5581 ctgctgcttt tttgcgatcg ccggaaccac ctgccgctcc agataatcgc gctgcatgcg
     5641 gttgagagcg cggccacgct cgggatgcca gatcacgtcg agatagaacg gcggaacctt
     5701 tgacatgtcg gattgccggc gcgctgcctc aaagtcccga taacagagca tcttttggtt
     5761 ggtgacgatg gtcggcgtat tggcaatgcc gtgctccagc gttgcttcga cgatcctatc
     5821 catccgccgt tcgtcgaccg cgtgccagtc gcacgagcgg ttgacgagcg tgtcgcgttc
     5881 cagcgtctgt ggctccggca cgccgaagaa atgctgcacc tcggcaatgc gcgcgtcctc
     5941 gtaagtgatg ccggcgggga cgtgccccat catcttgagg ccgtgcttgg cacatgcctg
     6001 ctccagggcg cgtatcatcg gctcggtcaa accttcgtag aatttcatga agctcgcgcc
     6061 gtcagccttg acgcggcacg cggcggcatc ggccgccgcc gggctcgcgt ccttcagcag
     6121 gatcgtgttc ttgaaagttg ccctgccggc gccgacaaag ggaccgcagg agaacacccg
     6181 cggcaccgga tgcgctgctt cgctgcttaa tcttcgcgcc gcggcgacgg ccgttccatc
     6241 gaggtcaccg gcttcgcgaa tgctggtcac gccgtgcaag aggtagagaa gcgctgcacc
     6301 ctgggtgagc ttcagggcgt tgtccggcgg caaatggaca tgcatatcga caagtccggg
     6361 cagggcgaaa catccggcaa gctcggacgg cggcacggcg tgcgtgtcgg aaattcccgc
     6421 aatcgcgccg ctggatatat caagagtcgc gcgggcgcgc cggtcgtgca tcgggttgat
     6481 gacggtgacg ccggcgatct ggaagcgacc ctcggcggaa caccggggag ggccaggtcg
     6541 gtgcagtcca agccggttga gaagacccat tgcaagaccc tccgaatcgc acctctgata
     6601 atcatgaata tgaatatata ttcatgttga agacaatgtg agcttgcatt catattgtca
     6661 acagggcaca cggaggcgtg atggccaagc cagcgagcga caagcggacc ggaccgcgca
     6721 aaccgcccac ccaggcgcgc agccgggcgc gcgtggaaaa cattctcaag gtggccgggg
     6781 cgttgctggg cgaagtcggc tacgacggcc tctcaaccaa tctgatcgcg gaacgcgctg
     6841 atgttccggt aggctcgatc tatcagttct tccagggcaa ggacgatatc gtcgcagcgc
     6901 tggtcgggca gtttcaggac cggattctcc gccttgcggg cgagcaactg gacgcggcaa
     6961 gcgctataca agaccgccgg gctttcatcg cgaagcttgt cgatggaata gccggcattc
     7021 aggccgaggc gtcggctttc gtgtgcgtgt tctccggcag ccagtcgcac gcgcgattcg
     7081 atggcctggc gcgggacctt cgccgcacgc tgacccgtca cctcgatcgc gtctttgccg
     7141 aagcgtttcc acggctgccg aaagacgacc gcggtcggat gcttgccgcg tggtcggata
     7201 tcacaggcgc cattatcgcc aacctcgatc ggagcaagcc gggagagcgc ggcaagctgc
     7261 tcgaagaact gaaaaccgtc cttgccgcgt atctcgacgc caaactggcg gcgtctcccg
     7321 cttgatccgg tcatcgctgc tctgcattcg acctatgcag gcgaacgctt ggggcaggca
     7381 atatgttgat ccagcgtcgg ccgcaaggca tgatctgccc gagatttgcg atgtgagatt
     7441 ggataacagg cgatgaggag gccggtggtc ggtgtgatcg gaaacgccta tcgcatcgaa
     7501 gatcgattcg cgacgcagct ggtcggagag cgaaatcttc gcgcggtggc cgaagtgtcg
     7561 ggcgcgctgc cgctgatgtt tgccgggtcc cccgacatca cggatatcgg cgcattgctg
     7621 gatgcggtcg acggcgtggt gttgaccggc gcccgcgcca atgttcaccc gacacgcttt
     7681 cgcaccgagc cggacccccg gcacgaaccc tacgatctcc atcgtgacga tctggcgctg
     7741 gctttgtcgg aagcatgcgt cgaccgcggc atacccctgt tcggtatctg ccgtggcctg
     7801 caggagatga acgtcgcctt tggcggctcg ttgcatccgg aaatccggga tttgcccggg
     7861 cgcatcaacc atcggatgcc gaggctggag aacggcgaaa tccaccccga tcccaacgtg
     7921 atctttgccg accggcatga agtcagtctg gttcccggcg gcgcctttgc gacgctgctc
     7981 ggttgcgaga ccatccgcgt caattcgctt cacggacagg gcatcctcga accgggcaag
     8041 cgggtcgtcg tcgagggcgt ggcggaagac ggaaccatcg aagcgatccg gattgctgac
     8101 gcaacgggtt ttgcgctcgg cgtccaatgg cacgcggagt acgatccgca gcgcaacccg
     8161 atcaaccgcg cattgttcca agcgttcggt gacgcattat tggtacgcaa gcaccgcagc
     8221 taggtttttg atccaacatg gatggatcag gaaccgccgt ttttcctctg ggtgagcacg
     8281 actttttccc gaaaaccgat agccactcca tcgatcgtgg cccgagggct tgcttttcgc
     8341 gattttgttc cagccgtcta cgccgcgctc gcttcagcct gagctctctg cccgcgcgcc
     8401 gcggatgttt ttgcacacga ttcttgtgcg cggggggcaa tcgccgcaat tgcacggagc
     8461 ggatcggtta tgctgattgc gctggtttga gtttgactga acactcaatg ttggacgaag
     8521 cttcgcattg gaatgtcttt attgttgggc cgccaagcgc aaaacatcga gcacaagccg
     8581 aaacaggcgg ttagcggcat caaactgtcg acccggctga cgctggcgat ggtctcgctg
     8641 gtcgtggtga cgacgagcgt gctaagtttc atcacatatc attccattac ccaggctgcg
     8701 gttccgagag cgcttgatcg gcttgccacc aaggccgtgt tgtgcgcgag caaactggag
     8761 tcggcgctga acgttgcgcg ccaggacgtc atggtcgttc aaggctcgac cggagtgata
     8821 caaatgggcg tggcgcgggc cgcaaacccg cttaagcctg tgtcggattt gccattacga
     8881 gaaagcatcg ccgcgcggct tttggcggcg ctggcggcca aaccggaata ttcccagttg
     8941 cgcgttatcg gcgtcgccaa tggcgggcgc gaactgctgc gcgtcgaccg caggggtccg
     9001 ggcggcgcac cccgcatcgt tcccgaagcc gaactcgttc agacgggcga acgcgactat
     9061 ttcaagcgcg caatcggcca gtcaaagtcc gacgttttcg tatctcccat cgagctgcaa
     9121 aaagaaggcg ctacggaagg tcctgcggtt ccgctgctgc acgtcggcgt acctctgttg
     9181 accccttccg gacaaccatg gggaatcagc gtgatcgatt tcgacatagg accgaaattc
     9241 gatcggattc gtgtcgaggg tggcagggag aatcaggtct ttgtgacgag cgcaaccggc
     9301 gactatctgc tccatccgga ccgaagtcgc gaattcgcgt ttgagaccgg agcggcggtt
     9361 cgcattcaac acgattttcc gggattcgat gaggcgatag cgggcggagc cgccaacgat
     9421 agcggaatct ggcaggatcg gagcggcgta cggttcgggg tgggttgggc gagggtgccc
     9481 ctcgcgggcg gggccggact tacgatcctg gtggccgcca cctattcaaa cctcacggtt
     9541 ggcctggcgg ccgtcaacag ttcggcgctg ataggcggcg cggtcgctat tctgctggcg
     9601 atttgcctcg cgatcgcaat tgcaaggtcg ctgtcgaaac cgttggtgca aataacccgt
     9661 gccgctgaag gtttgtcgcg gggagaattg atggctatgc cgtccgacgg cggccgggag
     9721 atcagcgctc tttccgcgac atttgccgag atggcaacac agatcaggac aaagcaatca
     9781 ctgctcgaaa ataccatcga aagtatcggc gactcggtcc tggtcgcgga cgaacgcggg
     9841 caaatcgtcg tcgccaatgc ggctgccaaa cgactgctcc agatcgttcc tggcgccggc
     9901 gtgagaggaa agttcagcta tttctatcct gacggcgtga cgcccatgcc ggcttcgagg
     9961 ttggcgctgg tgcgggcgct gggcggcgaa agcgttgacg atcaggaact cttcgtggtg
    10021 ccggaagctc cctgcgttcc ggcatatgtc gtcgcgaacg cacggccgct gagagacgaa
    10081 accggcgcca tacgcggggc gttaacggtt cttcgcaata tcaccgaaca caagcgagcc
    10141 caccagtctc tggtcgacag ccagcaaatg gcacaggcca tcatcaacac cgcactcgac
    10201 gccttcgtcc agaccgatga ggcgggcgtc atcctcgact ggagcccgca cgccgaggcg
    10261 atgctcggat ggacccgatc ggaagccatt ggtgcgaagg cggaagactt gatcgttccg
    10321 gagttgcagc gcgactcgaa caaccaatgg gtcaagcaat ttctgcatga cgtcggtatc
    10381 ggcgcgaagg gctggcgatt tgaagcaccg ttgctgcacc gcgacggcag cgagattttc
    10441 accgagatgt ctcttacgga gctgcgccgc ggcgaaggtc acatcataaa tgcgttcatt
    10501 cgggatatca cccagaagcg cgcggccgag gagcagctca tccaggcaca gaagatggag
    10561 tcggtaggcc agctgacggg cggtatcgcc catgacttca acaatatgct taccgttatt
    10621 acgggcacga ttgaaatcct ggcagaggct gttcggaacc aacctcatct cgcgcggatt
    10681 gtgacgttga tcagcgaagc ggccgatcgg ggatccgagc tcaccgcaaa tctgctcgca
    10741 ttcgcgagaa agcagccctt gcagcccgta gagatcgacg tcaatgcgct tgtgaacgaa
    10801 gtgggcagat tattgtcgcc gacgctgggc aggcagatcg agatcaagac cgctctgggc
    10861 ggcgacgtgt ggccggccct ggtcgatccg ggccaactca gttccgcgct cgtcaatctc
    10921 gccatcaatg cccgcgatgc catgcccgat ggcggaacgt tgacgtttgc gaccagcaac
    10981 gtcaccttgg acggacgtga cgcagcggca agcggcatcg atcgtgtgga cgactacgtc
    11041 gtcgtcgagg tgaccgacac gggcaccggg attccggaag caatccgcga caagattttt
    11101 gatccgtttt tttctaccaa ggaaaccggt caaggcaccg ggcttgggct gagcatggtc
    11161 tttggcttcg ccaagcagtc cggcggaaat atcgaagtcg acagcgagga aggccatggc
    11221 acgaccttca ggatttatct gccaaaggcg gactttgaag cctcgcagct tccgtcggcg
    11281 gacgatttgc aatcaacggg tggaaccgaa accatcctgt gcgtcgagga cgacgccagc
    11341 gtccgggcct acgttatcgc ccaactcgag agccttggct acaaggccat cgcggcctcg
    11401 aacgcggccg aagctcttgc aattgccgac ggaggcgcag agttcgatct tctgttcacc
    11461 gacgtcgtca tgccgggaag aatgaacggc aaacagctgg ccgaaaggat gaggctgcgc
    11521 cggccttcgc tgagggtcct gttcacctcc ggctacaccg atcacacgat cattcgggac
    11581 ggccgaatta tgcgagacgt ctttttcctg tcaaagccgt accgaaggcc gcagcttgcg
    11641 cggatggtgc gccgcagcct cgatgcacca ctggtcgctt tcgtcgtccc gagccaaccc
    11701 aagagcgcgt gaggcgccgc aaagccgccg gtccggaacc tcctcattca gtgccgtcac
    11761 gtcagaaaac agccctgccg gagcaggtag aattcatcga tatctgctac aatgcatccg
    11821 ccaatatcgc agcggtcagg gaatggtcgc gcaaccacgg ggaggatgcc gtgcaagacc
    11881 ggaaatggtc gaggcgcaaa gtgctcaagg tgtcggcggt atcggccgct gcgacgcttt
    11941 tcgccgagcc gcttgttgcc gccgcgccgc cggcgacgga ggtcacatca gcgttggtgg
    12001 aggcggcacg caaggaaggc aaggtctcgt tttacaccgc gctggagctc aacgtcgccg
    12061 aacgcctgag caggatgttc gaagctaaat atcccggcat tgccgtgcgc gtcgagcgtt
    12121 cgggcgcgga gcggattttc cagcgcatcg cgcaggagca ggccagcggc atcaatgcgg
    12181 tcgacgtcgc caacagcacc gatcccgcgc actatcccga ccggaaaaat cacggctggc
    12241 tcgcgcctta cgttcccgat gacgtcgcgc ggcattttcc cgccgatcag atcgacgcgg
    12301 acgggatgca cgccacctcc tgcgcatgga ccgaagcgat tggctacaat accagcctgg
    12361 tcaagcagga ggatgcgcca aagagctacg ccgacctgct cgatccgaag tggcaaggca
    12421 ggatcgtcaa gggtcatccg ggctacagcg gtgccatcat gaccgcgacc ttcgtgctcg
    12481 cgcgcgacct cggctggtcc tatctcgaaa agctcgcaca gcagaaggtg atgcaggtgc
    12541 agtcggcagc cgatccgccg aagaagatcc tgctcggcga gcgcgcgatc atggccgatg
    12601 gcaacgatta caatctggtg ctgctcaagg accagggtaa gccggtcgag gtggtgtatc
    12661 cgacagaagg atcgccgctg atcatcgttc cctccgggat attccagggc gcgcccaatc
    12721 caaatgccgc gcggctgttc cagagtttct tcttcagcgc cgagggccag caattgctgg
    12781 tcgacggttt cgcccatcgt tcgtttcatg cgctgatcaa ggagaaggcg ggccacacgc
    12841 cgctgtccac cttgaaattg ctcaaggccg accccgccgc ggtgcaggca caaagcgaag
    12901 agataaaggc gcgatacgcc aaagttttcg gagtgtgatc ttatgtgatg gggcgctcgg
    12961 tgcgaacgac acagaagcgt gttgccgcaa aataaaggct ctccttcagc agattgtgct
    13021 gcgacgcggc tagcatttgg gcgccgttgt cgttgacacg acagtcaatc ggctttgtat
    13081 tctggaataa tatatcatcc gacgaccgca ttcaaattgg cctttgaccg acgagagaat
    13141 tccatgtccc atatcaatcg aagagacgct ttgaagatgt ctgccagttt cgcgctggcg
    13201 gccgcggcag gcggattctc ctgcatcgag acggcctccg caggtcctat cgacgtgccg
    13261 acgatcgaca agctcagcgt ccgcgttctg gtcgacagcg ccagcgatat ctttttccgg
    13321 ccgcaggagg cgaacggcgt gaagacggag cccggccgct cggcggattc gacgcgcccg
    13381 ctgcatagcg aatgggggct gtcgctgctg ctggagccgc agcgcggcga tgacaagcgt
    13441 accttcctgc ttgactatgg ttggacgccc gaggtcatca acggcaacat ggacttgttg
    13501 aaagtcgacg cgtcgaagat cgacacgctc atcatgagcc acggccactt cgaccactgg
    13561 ggcggtctgc tcggatttct ggacaagcac cgcaaggatt tgcctgccga cctcacgatg
    13621 tatgccggcg gcgaggacaa tttctgccag cgctacgccc gggccgggca aggcgaactt
    13681 agcgactttg ggatgctcga ccgccgcgat atcgccaggc agaacgtcaa gctcgtgttg
    13741 tgtgagtcgc ccgtcgtgat cggcggccag gcattcacga caggcaagat caagcgcagc
    13801 agcatcgaaa aggtgcttcc gaacaccatg gtggagttca agctcaagga cggcgccgga
    13861 tgcaacgcga gccattattt gcccgctgaa atggaaggca agatcgtgcc cgatgagcac
    13921 atccatgagc acgccacgtg cttcaatttg aaggacaagg gtctcatcgt catcagctcc
    13981 tgcggccacg tcggtatcgt caattccgtg cgccaggcga tggaagtgtc gggcgtttcg
    14041 aagattcatg ccatcatggg aggctttcac cttgggcccg caccggcgga ttaccttacg
    14101 caggtcgtca gcgagatcgg caagttcaat ccggacgtcc tgattccgat gcattgcagc
    14161 gggctgaact tcacccaaga ggcgcagcgg cagatgccgg gcaaagttct gacaacgacg
    14221 accggcactc gaatcagctt cggcacctga ttcaggtcga agccatggtg ggttcaaggg
    14281 cgtgcgcatt ggttgtcatc gcgtctttgt tcagctgcgg cttgaccgcg gcggcggaac
    14341 agccatcagc cgcgcagatg atggatgatt taatgtacgg ccgtgggtcg gtcggtggac
    14401 cgttcacatt gaccgaccag gccggcaagc aacgaagcga caccgatttc cgcggcaagc
    14461 tgatgatcgt gtatttcggc tatacctatt gccccgatgt gtgcccaacc gatctgatgg
    14521 ccatcacgca ggcgttggac gccttgggtt cggccgccga aggggtccag ccgattttca
    14581 tcacgatcga tccggaacgg gacaccaacc tgctcgcgga ctacgtctct gcgttccatc
    14641 acagttttgt cggccttacc ggtccagcag aagaaattcg aaaggtcgcc aactcctaca
    14701 aggcgttcta cgtgaaggta ccggacgaac ggagcgggaa ttactcgatc gaccacgccg
    14761 gcgtcatcta cctgatgggg cggaacggcg agtatctggg gttcatgcct ccgcggacca
    14821 atcccgaccg gttgaccgag gtgttgcgca agaatcttgc caactagagc cttttccgtt
    14881 tcgatagaat cgaaacgggc tttagattct tcatttgacg cgttttcttg acgcgaaccg
    14941 gtctccaccc cggatcaagt ccggggcagg ctttcgctcg aaaacgctct gacgacgtcc
    15001 tcccgcaaaa caaaaggcgc ccccaccgga agcgcctttt gcgttttcag gccgtgagat
    15061 tcgcgggcgc gaaacccgcg aacgaacatc acagcgaata gtacatttcg aattcgaccg
    15121 gatgcggggt catttcgaag cgctcgactt cggtcatctt cagctcgata tagctgtcga
    15181 taaaatcgtc gtcgaacacg ccgccgttct tgaggaacgc gcgatccttg tcgagacatt
    15241 ccagcgcctc gcgcagcgaa ccgcacaccg tcgggatcgt cttgagctct tccttgggga
    15301 gatcgtagag gtccttgtcc atggccggac ccggatcgag cttgttcttg atgccatcca
    15361 gaccggccat cagcatcgcc gcaaagccga gatacggatt ggccatcgga tcggggaaac
    15421 gcacctcgac gcgcttggcc ttcggattgg aggtgtaggg aatccggcat gcggccgagc
    15481 ggttgcgcgc ggagtaggcg agcagcaccg gcgcttcata gcccggaacc agacgtttgt
    15541 aggagttggt cgacgggttg gtgaaggcgt tgatcgcctt ggcgtgcttg atgacgccgg
    15601 cgatgtagaa caggcaggtt tccgagaggt cggcgtattt gttgccggcg aataccggct
    15661 tgccttcctt ccagatcgac tggtgacagt gcatgcccga gccgttgtcg ccatagaccg
    15721 gcttcggcat gaaggtggcg gtcttgccgt agatgtgcgc gacctgatgg atacaatatt
    15781 tgtagatctg catctggtcg gccatcagcg tcagcgtgtc gaacttcatg ccgagttcgt
    15841 gctgggcgga ggcgacttcg tggtgatgct tctcgacctt gacgcccatc ttggccatcg
    15901 cgccgagcat ttccgagcgc atgtcctgca ccgaatcctg cgggggaacc ggaaagtagc
    15961 ccgctttggt ccggatgcgg tggccgaggt tgccgccctc gtattcggtg tccgagttga
    16021 tcggcagttc cgacgaatcc agcttgaagc cggtgttgta gggcgtggtc tggaaccgga
    16081 cgtcgtcgaa cacgaagaat tccgcctcag gtccgaagta aaccgtatcg ccaacgccca
    16141 tcgacttgac catcgcctcg gccttcttgg ccatgccgcg cgggtcgcgg ttgtagggct
    16201 cgccggtggt cggctccagc acgtcgcagg tgatcaccat ggtggtttcc gcgaagaacg
    16261 gatcgatcgt cgcggtgacc ggatcgggca tcaggcacat gtcggattca ttgatcgcct
    16321 tccagcctgc gatcgacgaa ccgtcgaaca tcaggccttc ggcgaaagtg tcctcctcga
    16381 tcatgccgat atcgaaggtc acatgctgcc acttgccgcg cggatcggtg aaacgcaggt
    16441 cgacgtactt gacgtcgttg tccttgattg atttcaggac atctttggcg gtcttcatga
    16501 ataccccttc tggcttacag gtcgtcgtga tccggccgcg ggcagcgacc ttgggtaatg
    16561 ctcattacaa ttggggcaag ctcaatacaa tatcctcaag acaatatcgg gatcctcaag
    16621 acaatatcgg gggtgtcggc gggctcgatt cagatggcgt cgagcccgga ttccccagtt
    16681 ctgatccgaa tggcttcctc gatgttcgaa acgaagatct tgccgtcgcc gatacgcccg
    16741 gtctgggccg cacgcctgat cgcgtcgatc gccctctcga ccaggtcatc cccgatcacg
    16801 atctcgattt tgaccttggg cagaaagtcc acgatgtatt cagcgccgcg atagagttcg
    16861 gcgtgcccct tctgccgtcc gaaacctttg gcttcggtga cggtgatgcc ctgcagtccg
    16921 acctcctgaa gtgcctcttt cacctcgtcg agcttgaatg gcttgatgat ggcttcgatc
    16981 tttttcactg aacgcctccc gggcacttcc atatttcaga ttgcaggttt gtttccacgt
    17041 ggcgcgtgga gcgcgggttg ttcgaccgtt gtccttgcga attgtcctgg cgggcagccg
    17101 gggaaacgtc aataaattgc cattgagcat gatcgtccaa agccggcttc ctcaaaagca
    17161 gggtctatgc caagttgcca aaacgcctga tttcgaaatg ttatcagact tttaacaggt
    17221 attcacatgg ggtcaggcgg ggcggtttgc cacagtcgag cctatgaatt aggcaaaaag
    17281 gtcaatcggc gtgcaatacc catgggtttg gtacggcgcg ggatggcaaa tgcctgagga
    17341 tgaggcgctt ggatcggaaa tttggcatgg aagttcttga tacggctgag atggagcgcg
    17401 ccgatcggct caccatcgcc gcgggtactc ctggctttgc actgatgcta agtgcaggtc
    17461 aggccgttgc ggaggccgcg atcgacctgg tggcggaggg gccgatgctg gtggtcgccg
    17521 gcagcggtaa taatggcggc gacggtttcg tggctgcggc tgagctcgcg gcgcgcggcc
    17581 gcgaggtctc ggtcatcctg ctgtgtgagc gggacagctt gcgaggcgac gcagcctcgg
    17641 cggcgcgcgg atggaaatat ccggtgctgc cgtgcaatcc ccaggcgatc ggcaagcccg
    17701 cgctgatcat cgacgccttg ttcggtgccg gtctgaaccg gccggtgaag ggcgatccat
    17761 acgacatgat cgaggcgatc aatgccaacg gcgcgccggt tctcag
//
LOCUS       DASWGM010000284         3607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_436945,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000284 DASWGM010000000
VERSION     DASWGM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436945"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /gene="pgk"
                     /locus_tag="VGA15_08345"
     CDS             complement(<1..161)
                     /gene="pgk"
                     /locus_tag="VGA15_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX9587741.1"
                     /translation="MSKTFRTLDDVDVKGKRVLLRVDLNVPMENGRVSDATRLERVAP
                     TITEISDKGG"
     gene            complement(315..1322)
                     /gene="gap"
                     /locus_tag="VGA15_08350"
     CDS             complement(315..1322)
                     /gene="gap"
                     /locus_tag="VGA15_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX9587742.1"
                     /translation="MAVRVAINGFGRIGRNILRAIAESGRKDIEVVAINDLGPVETNA
                     HLLRYDSVHGRFPGTVTVDGDSISLGNGKIKVTAVRDPNTLPWKDLGIDIALECTGIF
                     TAKDKASSHLTAGAKRVLVSAPADGADATIVFGVNHDKLNKDHLVVSNGSCTTNCLAP
                     VAKVLHDTVGIETGFMTTIHAYTGDQPTLDTLHKDLYRGRAAAMSMIPTSTGAAKAIG
                     LVMPELAGKLDGVSIRVPTPNVSVIDLKIVAQRKTDVKEINEAMKRASEQQLKGILGY
                     TTAPNVSIDFNHDPHSATFHMDQTKVQNGTLVRVMAWYDNEWGFSNRMADTAVAMGKL
                     L"
     gene            complement(1468..3450)
                     /gene="tkt"
                     /locus_tag="VGA15_08355"
     CDS             complement(1468..3450)
                     /gene="tkt"
                     /locus_tag="VGA15_08355"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9587743.1"
                     /translation="MTQVDPSRMANAIRGLAMDAVEKAKSGHPGLPMGAADIATVLFT
                     QFLKFDAADPKWPDRDRFILSAGHGSMLLYSLLYLTGNTDMTLDQLKHFRQLGSLTPG
                     HPENFHTKGIETTTGPLGQGIATSVGFALAERMLAAEYGRKVVDHHTYVLASDGDLME
                     GVSQEAIALAGHWKLNKLIVLYDDNGITIDGPTSISDSVDQVKRFKSAGWQAELIDGQ
                     DQQAIAAAITRAQKSNKPSMIACKTTIGFGAPTKAGTAKAHGEALGAEELKGAKEKLG
                     ISLEPFSVPDDVLKSWREAGSRGDAARKEWEARFAELPARRRTEFERRLRHERPAALA
                     KAFKAHKKALLETPQNIATRKSSESVIDAIVLAMPEFVAGSADLTGSNNNKAKSAIAF
                     SAKTPKGRFIHYGIREHGMAAAMNGIFLHGGFAPNGATFLVFTDYARPAMRLAALMGT
                     GVVYVMTHDSIGLGEDGPTHQPVEHLAALRAIPNMRVFRPCDAVEVAECWELALNRID
                     GPTVLALTRQNLPQLRTDAPADSPCSHGAYQLVAPQGEAKVSLFASGSEVEIAVAAQK
                     QLGERGIASRVVSVPSLELLLAQPVEKRKAIIGNAPVKVAIEAAVRFGWDAVIGPDGA
                     FVGMHGFGASAPVKDLYQHFGITAEAAVNAALKLLG"
BASE COUNT          618 a         1122 c         1162 g          705 t
ORIGIN      
        1 ccgcccttgt cggaaatctc ggtgatggtg ggtgcgacac gttcgagccg ggtcgcgtcg
       61 ctgacgcggc cattctccat cggcacgttg agatcgacgc gcagcagcac gcgcttgccc
      121 ttcacgtcga cgtcatcgag ggtgcggaat gttttggaca tctctgactc ttgtcccggg
      181 cgcagcgcag cgcgaagcgg tgcgctgcag aaccgggacc catgttggtg ccaggtcccg
      241 gctcagcgga acagcgtact ggacgatgct acgcatcgcc aggagtacgc tgcaccgcgt
      301 ccgggacgcg agacttacag cagcttcccc atcgccacgg cggtgtcggc catgcggttg
      361 gaaaaacccc attcattatc gtaccacgcc atcacccgca ccagcgtgcc gttctgcacc
      421 ttggtctggt ccatgtggaa ggtggcggaa tgcggatcgt ggttgaaatc gatcgagacg
      481 ttgggcgcgg tggtatagcc caaaatgccc ttgagctgct gctcggaagc gcgcttcatc
      541 gcctcgttga tttccttcac gtcggtcttg cgttgcgcga cgatcttgag atcgatcacc
      601 gaaacgttcg gcgtcggtac acggatcgat acgccgtcaa gtttgccggc aagctccggc
      661 atcacgaggc cgatcgcctt ggcggccccg gtggaggtcg ggatcatcga catcgcagcc
      721 gcccggccgc gatagagatc cttgtgcagg gtatcgagcg tcggctgatc gccggtatag
      781 gcatggatcg tggtcatgaa gccggtttcg atgccgacgg tgtcgtgcag cacttttgcg
      841 actggcgcga ggcagttggt cgtgcacgaa ccattggaga cgaccaggtg atccttgttc
      901 agcttgtcgt ggttgacccc gaacacgatg gtcgcatcgg cgccatccgc gggcgccgag
      961 accaacactc gcttggcgcc ggcagtcaga tgcgaggagg ccttgtcctt cgccgtgaag
     1021 atgccggtgc attccagcgc gatgtcgatg ccgagatcct tccacggcaa ggtgttggga
     1081 tcgcgcaccg cggtaacctt gatcttgccg ttgccgaggc tgatcgagtc gccatccacg
     1141 gtcacggtgc cggggaagcg gccatgcact gaatcgtagc gcaacagatg ggcgttggtc
     1201 tcgaccgggc cgagatcgtt gatggcgacc acttcgatat ccttgcggcc ggactcggcg
     1261 atggcccgca gtatgttgcg gccgatgcgg ccgaacccgt tgatcgcgac ccggactgcc
     1321 atctgtctct ccttcaatta ttttgttacc ccccgcggaa ggcgcttgag ccgtccacgc
     1381 gagtgcttgc ggcggacgcc cggttcggcc gggcgggagc ggataatatg cccgttaggt
     1441 agtctttttt tcggccaacc tcaaccgtca gcccaatagc ttcagcgcag cgttaactgc
     1501 cgcctcggcg gtaattccga aatgctggta aaggtccttg accggcgcgc tggcgccgaa
     1561 accgtgcatg cctacaaatg cgccatccgg cccgatcacc gcatcccagc cgaaccggac
     1621 cgcggcctcg atcgcaacct tgaccggggc gttaccgata atggccttcc gtttctccac
     1681 aggttgcgcc agcagcaatt ccagcgaagg caccgagacc acccgcgacg cgatgccgcg
     1741 ctcgccgagc tgcttctgcg cggcaaccgc gatctcgact tcggagccgg aggcgaacag
     1801 cgataccttg gcctcgccct gcggtgcgac cagttgatag gccccgtggc tgcaggggct
     1861 atcggcaggc gcatcggtcc gcaattgcgg caggttctgg cgggtcagcg ccagcaccgt
     1921 cggaccatcg atccggttga gcgccagctc ccagcattcc gccacctcga ccgcgtcgca
     1981 gggacggaac acgcgcatgt tcggaatggc gcgaagggct gcgagatgtt cgaccggctg
     2041 atgcgtcggt ccgtcttcgc cgagaccgat cgaatcatgg gtcatcacat agacgacgcc
     2101 ggtgcccatc aacgccgcga gccgcatcgc cggccgggca taatcggtga agaccaggaa
     2161 agtcgcgcca ttcggtgcga atccgccatg caaaaagatt ccattcatgg ccgccgccat
     2221 gccgtgctcg cggatgccgt aatggatgaa acggcctttc ggcgtcttgg ccgaaaaggc
     2281 gatcgccgat ttcgccttgt tgttgttgga gccggtgaga tcggccgagc ccgccacaaa
     2341 ttccggcatc gccagtacga tcgcgtcgat gacggattcg gacgatttgc gggtggcgat
     2401 gttctgcggc gtttcaagca gcgccttctt gtgggccttg aaggctttcg caagggctgc
     2461 cggccgctcg tgccgcaagc gacgttcgaa ctcggtgcgc cggcgggcgg gcagttcggc
     2521 aaagcgagcc tcccactcct tgcgcgcggc gtcgccgcgg ctgccggctt cgcgccagct
     2581 tttcagcacg tcgtcgggca ccgagaacgg ctccagcgaa atgccgagtt tttccttggc
     2641 gcccttgagc tcctcggcgc ccagcgcctc gccatgcgcc ttggcggtgc cggccttggt
     2701 cggcgccccg aaaccgatgg tggtcttgca ggcgatcatc gaaggcttat tggatttctg
     2761 cgcccgcgtg atcgcggcgg cgatcgcctg ctgatcctga ccgtcgatga gttcggcctg
     2821 ccagccggcg gacttgaacc gttttacctg gtcgaccgag tcggagatcg aggttggacc
     2881 gtcgatcgta atgccgttgt cgtcatacag cacgatcagc ttgttgagct tccagtgccc
     2941 ggcgagcgcg atcgcttcct gcgacacgcc ttccatcagg tcgccgtcgg acgccagcac
     3001 ataggtgtga tggtcgacaa ctttcctgcc gtactcggcg gcgagcatcc tctccgcgag
     3061 cgcgaagccg accgacgtcg cgatgccctg gccgagcggg ccggtcgtgg tttcgatgcc
     3121 cttggtgtgg aaattttccg gatggcccgg cgtcagcgat cccagctggc ggaagtgctt
     3181 gagctggtcg agcgtcatgt cggtattgcc ggtcaggtac aacagcgaat acagcagcat
     3241 cgaaccgtgc ccggccgaca ggatgaatcg gtcgcggtcc ggccatttcg ggtcggcggc
     3301 gtcgaatttc aggaactgtg tgaacagcac ggtggcgatg tcggccgcac ccatcggcag
     3361 gccgggatgg ccggatttcg ccttctcgac ggcatccatc gccagcccgc gaatcgcatt
     3421 ggccatacgg gaaggatcga cctgcgtcat gatgaaaatc cgcctgaaat gagctgccct
     3481 ggaggtgatt cgcgctggcg gcgaatagcc gggcaggact gtggcaacgg gaagccgggg
     3541 ctggatagca tctcggttcc cggagtccaa ggcaggcaaa cgcgccatgg ggccgaaaag
     3601 ttgcttt
//
LOCUS       DASWGM010000285         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_857909,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000285 DASWGM010000000
VERSION     DASWGM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857909"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VGA15_08360"
     CDS             complement(<1..557)
                     /locus_tag="VGA15_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEX9587744.1"
                     /translation="MSDDNKQGLQSAFRRKLLMGMAAIPALTMLPRPSFAEAPATSAI
                     NTTGLAVTDTEVTCGILHSATGTMAISETGSIEAEKLAIEQINATGGILGRKIKFIQE
                     DGASDWPTFAEKAKKLLVNDKVAAIMGCWTSASRKAVLPVVEQYNGMLYYPTFYEGLE
                     QSKNVIYTGQEATQQILAGLNWIAK"
     gene            712..>2024
                     /locus_tag="VGA15_08365"
     CDS             712..>2024
                     /locus_tag="VGA15_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX9587745.1"
                     /translation="MAGRQRIDRVRRQYNQWVANQTLEDYALRFTAKSARRWSAARVA
                     NTALGAISFLALEAIGGTITLNYGVTNAAAAILVVSVIIFCCGLPIAYHAAKCGIDID
                     LLTRGAGFGYIGSTITSLIYASFTFIFFAIEAVILATALEMCFGIPRPLGYLISAVVI
                     IPLVTHGITLISRFQLWTQPVWIILHILPFAAIAYANPYSFAEWTKFPGDHGDPAGHL
                     DLLLFGTAASVVFALVAQIGEQVDFLRFLPRDRRTSKRSWWIALLSAGPGWIILGALK
                     LLAGSFLAYFALNHGVSAEHAAEPAHMYLEAFRYVLSQPDLALALTGTFVILSQLKIN
                     VTNAYAGSIAWSNFFSRLTHSHPGRVVWLVFNVIVALLLMEIGVYKALEQTLALYSNV
                     AIAWVGALVSDLVVNKPLGLRPPHMEFKRAHLYDINPVGVGAMTIA"
BASE COUNT          333 a          632 c          608 g          451 t
ORIGIN      
        1 tccttggcga tccagttcag gccggcgagg atttgctggg tcgcctcctg gccggtgtag
       61 atgacgttct tggactgctc gagaccttca tagaaggtcg ggtagtagag catgccgtta
      121 tattgctcga cgacgggaag caccgccttg cgggaggccg aggtccagca gcccatgatc
      181 gccgcgacct tgtcgttgac gagcagtttc ttggcttttt ccgcgaaggt cggccagtcg
      241 ctggcgccgt cctcctggat gaacttgatc ttgcggccga gaataccgcc ggtggcgttg
      301 atctgttcga tggcgagttt ctcggcttcg atcgagccgg tttccgaaat cgccatcgtg
      361 ccggtggccg aatgcaggat gccgcaggtg acttcagtat cggtaaccgc gagacccgtg
      421 gtgttgatcg cggaggttgc cggagcttct gcaaaagacg ggcgaggcag catggtcaag
      481 gcgggaatcg ccgccattcc catcagcagc ttgcggcgaa atgccgactg cagcccctgt
      541 ttgttgtcgt ctgacatatt caccccattg ctgttcgagt acgcgtgagt ggcggggttg
      601 aggattgctg aaattgtgca gtgcacagat acgcaagatc gcgtatattg caccgcaaaa
      661 tagcgacgta ggttttcgtt cgggttttcc ggcggggtca ggagtgcaga agtggcaggg
      721 cggcagcgga tagaccgcgt caggcgccag tataatcaat gggttgccaa ccagacgctg
      781 gaagactacg cgctgcgctt cacggccaaa agcgcgcggc gctggtccgc cgcccgggtc
      841 gccaacaccg ctctgggcgc gatctcgttc ctggccctgg aggcgatcgg cggaacgatc
      901 acccttaact atggcgtgac caacgccgcc gcggccatcc tcgtcgtcag cgtcattatc
      961 ttctgctgcg gtttgccgat cgcctatcat gccgccaaat gcggcatcga catcgatctg
     1021 ttaacgcgcg gggcgggctt cggctacatc ggctcgacca tcacgtcgct gatctacgcg
     1081 tccttcacct tcatcttctt tgcgatcgag gcggtcattc tcgcaaccgc gctggagatg
     1141 tgcttcggaa tcccgcgtcc cctcggctat ctcatcagcg ccgtcgtcat catcccgctg
     1201 gtgacgcacg gcatcacgct gatcagccgc ttccaattgt ggacgcagcc ggtctggatc
     1261 atccttcaca tcctcccctt tgcggcgatc gcctatgcca atccctactc ctttgcggag
     1321 tggaccaaat ttcccggcga ccacggcgat cccgcgggtc atctcgatct cctgctgttc
     1381 ggcaccgccg cgtcggtggt gttcgcgctg gtggcgcaga tcggcgaaca ggttgacttc
     1441 ctgcgcttcc tgccgcgcga ccggcgaacc tcgaagaggt cgtggtggat tgcgctgctc
     1501 agcgccggtc cgggctggat cattctcggc gcgctcaagc tattggccgg atcgttcctc
     1561 gcttacttcg cgctcaacca cggcgtctcc gccgagcatg cggccgaacc cgcgcacatg
     1621 tatctcgagg cttttcgcta cgtgctgtcg caaccggatc tcgcattggc gttgaccggc
     1681 acctttgtga tcctctcgca gttgaagatc aacgtcacca acgcctatgc cggctcgatc
     1741 gcatggtcga acttcttttc ccgcctgacc cacagccatc cggggcgtgt ggtctggctg
     1801 gttttcaacg tgatcgtcgc gctgctgctg atggagatcg gcgtctacaa ggcgctcgag
     1861 cagacgctgg cgctttactc caacgtcgcc atcgcctggg tcggagcgct ggtctccgat
     1921 ctcgtcgtca acaaaccgct cggcttgcgc ccgccccaca tggagttcaa gcgcgcgcat
     1981 ctctacgaca tcaatccggt cggcgtcggc gcgatgacga tcgc
//
LOCUS       DASWGM010000286         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2522154,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000286 DASWGM010000000
VERSION     DASWGM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522154"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="VGA15_08370"
     CDS             <1..97
                     /locus_tag="VGA15_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEX9587746.1"
                     /translation="EAFDGERRIGEGRHARGLVNLATFNKRLGAR"
     gene            complement(408..1754)
                     /locus_tag="VGA15_08375"
     CDS             complement(408..1754)
                     /locus_tag="VGA15_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX9587747.1"
                     /translation="MADERLFEDLPEQERPQAEIRGAVRLRVPERSQIGMQVAALDDL
                     ISEDHPVRSVWAFVEGLDLSALHDVIKAREGQPGHPPAAPELMLALWLWATVDGVGSA
                     RQLDRLCRDHLVYRWLCGGVSMNYHSLSTFRIAHMAVLERLLAQGVASMVQAGLVSLD
                     TLAQDGLRVRASAGASSFRRHARLDDLLTAAQTRVERLRAEVETDPTAGDRVRRAAKE
                     RGAREREARIAAAQQRMKELEAERERREKTNKKEVAKQKEPRASTTDAEARVMKMADG
                     GWRPAYNMQIISSPEHQVIVAVDIDTTGSDRGLAQPGIETVHAEGYTPSNYLVDGGFT
                     KNDDIEWAHAKDIKMWCPPGQTRHGTDPYAPKKDDGVGVADWRQRMKGEEDKKGEALY
                     RLRAEHECINAHARRMGLRQLSLRGKIKARIHLLWFAVARNMMRFLALHAKAQAAA"
     gene            complement(1959..>2364)
                     /locus_tag="VGA15_08380"
     CDS             complement(1959..>2364)
                     /locus_tag="VGA15_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9587748.1"
                     /translation="YDAAAEQDGTRLTVVGDAEVLVTGDRDLIFDAIANLVDNAIKHG
                     RPGGQVVVANENIDGGPVISIADDGPGIPAGEYEHVFKRFYRLEHSRYTPGNGLGLSL
                     VAAVARLHGARIEMLDNSPGLKFKLWFSAPAG"
BASE COUNT          421 a          800 c          698 g          445 t
ORIGIN      
        1 tgaagccttc gacggcgaac gccggatcgg cgagggccgt catgcgcgcg gtctggtcaa
       61 tcttgcgacc ttcaacaagc ggttgggggc caggtagccg aatacaccga cctcatcctg
      121 aggagcggcc tcttggccgc gtctcgaagg atggcaacag cccgtggccc atccttcgag
      181 acgccgcttc gcggctcctc agagtttgag aatgtttcgc gttcgctgcg agaatcttgc
      241 atctgatggg ccggattatg ccgcccccaa atgtttcggc tgagccgggc gcccgaacga
      301 cggtgatctg gccgaagctc cgcaacctgc aagagccagg ctatttcagc gccaagccgg
      361 ctgacggccc atgatagatg atagatgcct gctggcgtgc tccccggtta tgctgccgcc
      421 tgcgccttgg cgtgcaaggc gaggaagcgc atcatgttgc gagcgaccgc gaaccacaac
      481 agatggatgc gggccttgat cttgccgcgc agcgagagct ggcgcaggcc catgcggcgg
      541 gcatgggcat tgatgcattc gtgctcggcg cgcagtcggt agagcgcctc gcctttcttg
      601 tcttcctcgc ccttcatgcg ttgacgccaa tccgcgacgc cgacaccgtc atctttcttc
      661 ggcgcatagg ggtcggtgcc atgcctggtc tgccccggcg ggcaccacat ctttatatcc
      721 ttggcatggg cccattcgat gtcgtcattt ttggtgaagc cgccatcgac cagataattg
      781 gaaggtgtgt agccttcagc gtgcacggtc tcgatccctg gttgggccag gccgcgatcc
      841 gaaccggtgg tgtcgatgtc gaccgcaacg atcacctggt gttccggcga cgatatgatc
      901 tgcatgttgt aagccgggcg ccatccgcca tccgccatct tcatcacccg cgcctcggcg
      961 tcggtggtcg aagcgcgcgg ctccttctgc ttggccactt ctttcttgtt ggtcttctcg
     1021 cgccgttccc gctcggcctc cagttccttc atgcgctgct gcgccgccgc gatccgcgcc
     1081 tcgcgctcgc gggcgccacg ctcttttgcc gcacgccgga cacggtcgcc ggccgtcgga
     1141 tccgtctcga cttcagcacg caggcgctcc acccgcgtct gcgccgcggt gagcagatca
     1201 tcaagccgcg cgtgccggcg gaacgaactc gccccggcac tcgcgcgcac ccgcaagcca
     1261 tcctgcgcca acgtgtcgag cgacaccaac ccagcctgca ccatcgaggc gacgccctgc
     1321 gccagaagcc gctccagaac cgccatatgg gcgatccgga aggtcgacag gctgtgatag
     1381 ttcatcgaca ccccgccgca taaccagcga tagaccagat gatcgcggca cagccggtcc
     1441 agttggcggg cgctgcccac cccatcgacc gtggcccata gccaaagcgc caacatcagt
     1501 tccggcgctg ctggcggatg tccgggctgg ccctcgcgcg ccttgatgac gtcgtgcaac
     1561 gccgacagat ccagcccctc gacgaacgcc caaaccgagc gtaccggatg atcctccgaa
     1621 atcagatcgt ccaatgccgc cacctgcata ccgatctgac tgcgttccgg cacccgcagc
     1681 cgcaccgcgc cgcgtatctc ggcctgcggt ctctcctgct cgggtaaatc ctcgaacaat
     1741 cgttcgtcag ccatcgccag cccctgcttc gcgctcaatg ccatggaatc acagcacggc
     1801 aactctggca aatgcacttg ccgaaagatt cacaagctct caggatgagg acccaaaccc
     1861 tcatggtgag gagcgcggca acgccgcgcg tctcgaaccc atgaggccgt tggatctccg
     1921 cgtgcgccac aggcggcttt cgcttgaaaa cgctctaact aaccggctgg cgctgaaaac
     1981 cagagcttga acttgagacc gggcgagtta tcgagcattt cgatccgcgc gccatgaaga
     2041 cgggcgacgg cggcgaccag actgagcccg agaccgtttc caggcgtgta gcgactgtgt
     2101 tcgagccggt aaaagcgctt gaaaacatgc tcgtattcgc cggcgggaat tccgggaccg
     2161 tcatcggcga tcgagatcac cggcccacca tcgatgtttt cgttggcaac gacaacttgc
     2221 cctccggggc ggccatgctt gatcgcattg tcgacgagat tggcgatggc atcgaagatc
     2281 agatcgcgat cgccggtcac cagcacctca gcgtcgccga caacggtgag acgcgtgccg
     2341 tcctgttcgg cggcggcatc gtac
//
LOCUS       DASWGM010000287         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1133241,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000287 DASWGM010000000
VERSION     DASWGM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133241"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VGA15_08385"
     CDS             complement(<1..125)
                     /locus_tag="VGA15_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEX9587749.1"
                     /translation="MCEPMAKTDQPTTIKKYANRRLYNTGTSTYVTLEDLASMVK"
     gene            420..1598
                     /locus_tag="VGA15_08390"
     CDS             420..1598
                     /locus_tag="VGA15_08390"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9587750.1"
                     /translation="MSDDVVIVSAARTPVGSFNGAFATMPAHDLGAIAIKAALERGGI
                     EPARVSEVIMGQILTAAQGQNPARQASIAAGIPVESPAWGVNQLCGSGLRTVALGYQA
                     LLNGDSEIVVAGGQESMSMAPHAQYLRGGVKMGPVEFIDTMIKDGLWDAFNGYHMGNT
                     AENVARQYQITRAQQDEFAVGSQNKAEAAQKAGRFKDEIVPVTIKTRKGDIVVSDDEY
                     PRHGATIEAMGKLKPAFEKEGTVTAGSASGINDGAAAVVLMTAKQAAKEGKKVLGRIV
                     SWGQAGVDPKIMGTGPIPASRAALKKAGWSIGDLDLIEANEAFAAQACAVNKDLGWDT
                     TKVNVNGGAIAIGHPVGASGARVLVTLLHEMQKRDAKKGLATLCIGGGMGIAMCIARD
                     "
     gene            1773..2498
                     /gene="phbB"
                     /locus_tag="VGA15_08395"
     CDS             1773..2498
                     /gene="phbB"
                     /locus_tag="VGA15_08395"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX9587751.1"
                     /translation="MARVALVTGGTRGIGAAISKALKAAGYKVAASYAGNDAAAEKFK
                     AETDIPVYKWDVSSFDACAAGVKQVEADLGPVDVLINNAGITRDGAFHKMTLEQWNAV
                     INTNLGSLFNMTRQVIEGMRARKFGRVINISSINGQKGQFGQVNYSAAKAGDIGFTKA
                     LALENAKGGVTVNAICPGYINTEMVQAVPKEVLEKSILPLIPVGRLGEPDEIARAVVF
                     LAADEAGAITGSTLTINGGQYMV"
     gene            2717..>2908
                     /locus_tag="VGA15_08400"
     CDS             2717..>2908
                     /locus_tag="VGA15_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9587752.1"
                     /translation="MTNLTTSRTATLIGLTAILMWSLLTVLTVATGKIPAFQLAAMTF
                     AIGALVGSLTWIGRPDAISA"
BASE COUNT          609 a          870 c          916 g          513 t
ORIGIN      
        1 tccttcacca tcgacgcgag gtcttcgagc gtcacatagg tgctggtgcc ggtattatag
       61 agccgccggt tcgcatattt cttgatcgtg gtcggttggt ctgttttcgc catgggctca
      121 cacatgcaca ccgaggagga cgggaacccg acggcatcgc cgaagggcaa gcgagcaacg
      181 caatcgcaac aaagtaagca ttttcaactg agttcggcta ccgttttgtg cgacacggtt
      241 aattccaaag cataggcact gctcaggaaa atgccaagaa cgctattttg aatgcgccgc
      301 ggcgggaatt cgccctctgg ccgattgaca tgcctcaact acgttaacag gataacgtga
      361 gagacaggcc tgccatccgg ccgcccgccg tcaagaatcc tcaagcccag gagatgtcca
      421 tgtcagacga tgtcgtcatc gtcagcgccg cccgcacccc ggtcggcagt ttcaacggcg
      481 cgttcgccac catgcccgcc cacgatctcg gcgcgatcgc catcaaggca gcccttgagc
      541 gcggcggcat cgagccggcg cgggtgtccg aggtgatcat gggccagatt ctgaccgcgg
      601 cgcagggcca gaacccggcg cgtcaggcct cgatcgccgc cggtatcccg gtggaaagcc
      661 cggcctgggg cgtcaatcaa ttgtgcggtt cgggcctgcg cacggtggcg ctgggctatc
      721 aggcgctgct caacggcgat tccgaaatcg tcgtcgcggg cggccaggaa tccatgagca
      781 tggccccgca cgcccaatac ctgcgcggcg gcgtcaagat gggcccggtc gaattcatcg
      841 acaccatgat caaggacggc ctgtgggacg ccttcaacgg ctaccacatg ggtaacaccg
      901 ccgagaacgt cgccaggcag tatcagatca cgcgcgccca gcaggacgaa ttcgcagtcg
      961 gctcgcagaa caaggccgag gccgcgcaga aggccggccg cttcaaggac gagatcgttc
     1021 cggtcaccat caaaacccgc aagggcgata ttgtcgtcag tgacgatgaa tatccccgtc
     1081 acggtgcgac gatcgaagcc atgggcaaac tcaagccggc cttcgaaaag gaaggtaccg
     1141 tcaccgccgg cagcgcttcc ggcatcaatg acggcgccgc cgcggtggtg ctgatgaccg
     1201 cgaagcaggc ggccaaggaa ggcaagaaag tactggggcg gatcgtgtcg tggggccagg
     1261 ccggcgtcga tcccaagatc atgggcaccg gccccatccc ggcctcgcgc gccgcgctga
     1321 aaaaggccgg ctggagcatc ggcgacctcg atttgatcga ggcaaatgag gccttcgcag
     1381 cgcaggcctg cgcggtcaac aaggacctcg gctgggatac gaccaaggtc aacgtcaacg
     1441 gtggcgcgat cgcgatcggc caccccgtag gcgcctccgg cgcccgcgtg ctggtgacgc
     1501 tgctgcatga aatgcagaag cgcgacgcca aaaagggcct cgccactttg tgcatcggcg
     1561 gcggcatggg catcgcgatg tgcattgcac gcgactaatc atgcggcgtt gcctgatgaa
     1621 aagatcatct cgcagctgcg taaagtcttg ctaaagtgaa acgcccggct cttgtgccgg
     1681 gcgttttatt cttgatgttg gtcaactggc gcatttaagt cgacgttaca aaagaagtgt
     1741 agtccaaaaa tacgccgagg gaaggattcg acatggcacg tgtagcattg gtgacggggg
     1801 gaacgcgggg cataggtgca gccatcagca aggcgctgaa ggccgccggc tacaaggtcg
     1861 ccgccagcta tgccggcaac gacgccgcgg cggaaaaatt caaggccgag accgacattc
     1921 ccgtctacaa atgggatgtc agttcgttcg atgcctgcgc cgcgggcgtc aagcaggtcg
     1981 aggccgacct cggcccggtc gacgtgctca tcaacaatgc cggcatcacc cgggatggcg
     2041 cattccacaa gatgacgctc gagcaatgga acgcggtgat caacaccaat ctcggctcgc
     2101 tgttcaacat gacgcgtcag gtgatcgaag gcatgcgcgc gcgaaaattc ggccgcgtca
     2161 tcaacatctc ctcgatcaac ggccagaaag gccagttcgg ccaggtcaat tattccgccg
     2221 ccaaggccgg cgatatcggc ttcaccaagg cgcttgcgct ggagaatgcc aagggcggcg
     2281 tcacggtgaa tgcgatctgc cccggctaca tcaacaccga gatggtgcag gcggtgccga
     2341 aggaggtgct ggagaaaagc attctgccgc tgatcccggt gggtcgcctc ggcgaacccg
     2401 acgagatcgc ccgcgccgtg gtgttcctcg ccgcggacga agcgggagcg atcaccggct
     2461 cgacgctcac gatcaatggc ggacagtata tggtgtgaat tgttgtgctc gtcattgcga
     2521 gttccgtacc ggccttgggg ccgaccctga tggatgaaag tgtggtgaat caaggcgata
     2581 gggctcgggg agttttccca ctttcttcct aggagcactt gcgacgaagc aaaccagcta
     2641 tctttacgcc tgaaagatgg attgcttcgc ggagcttgtc atcgggcggc gcgttgcgcc
     2701 gacccgttgg cccgcaatga cgaatttgac gacctcacgc accgcgacgc tgatcggcct
     2761 gaccgcgatc ctgatgtggt cgctgctgac ggtgcttacg gtggctaccg ggaaaattcc
     2821 cgcatttcaa ctggccgcga tgacgttcgc gatcggcgcg ctggtgggat ctctgacatg
     2881 gatcgggcgg ccggatgcca tcagcgca
//
LOCUS       DASWGM010000288         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2384777,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000288 DASWGM010000000
VERSION     DASWGM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2384777"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VGA15_08405"
     CDS             complement(<1..710)
                     /locus_tag="VGA15_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587753.1"
                     /translation="MKVNRRRFLQGTAAAAATGAFGSSAWAEDPINVASIHDLSGGLD
                     IYGKPMVDAITLAVEEANAAGGLLGRQIKLINYDTQSNMQLYTQFAQQAALKDKVAVV
                     HGGITSASREVIRPVLDRFKTLYFYNTQYEGGVCDRNQFDTGVTPAQTVEKLVPYAMK
                     KWGKKVYVIAADYNYGQITSQWVKKYVTENGGEVPSIDFFPLDVTNFGSTISKIQAAK
                     PDFVWSALVGGAHISFYR"
     gene            complement(739..1737)
                     /locus_tag="VGA15_08410"
     CDS             complement(739..1737)
                     /locus_tag="VGA15_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX9587754.1"
                     /translation="MTSASWLKTSIMAKRGLAKGEAGKRHELSIAAQGDFHYVYGPYA
                     RPVLTINPGDVVVVETEDAFGGVITGEQDSPTAKLRFPFLNPQCGPVAVSGVEKGDCL
                     AVQIHAVETRGAQPAGTTCIIPEFGGLVGTASTAMLNAPLPERVRKMHVDRDGVRWSD
                     KIILPYEPFIGTIGVSPEIEAISSLQPDYHGGNMDLPDVAPGAILYFPVHTKGGLLYV
                     GDCHATQGDGELSGVAIEQRATVTLQVDVIKNWSFAWPRLETENFLMTIGSARPLEDA
                     ARIAYRELVRWMSADYGFDEIDAYMLLSQAGRIRLGNMVDPKYTMGASILKKYLVS"
     gene            complement(1752..>2114)
                     /locus_tag="VGA15_08415"
     CDS             complement(1752..>2114)
                     /locus_tag="VGA15_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9587755.1"
                     /translation="SGKDYQKIVLRREAFKGMVKALFEKVDLLAVPAQTFAAPTLTKM
                     ATLGENPELIAGLLRFSCPFDMTGSPTITLSGGFTESGGPVAFQFVGRHLDEARLVAA
                     GDAFQRVTDWHRCHPSLG"
BASE COUNT          404 a          637 c          641 g          432 t
ORIGIN      
        1 tgccgataga acgagatatg cgcgccgccg accagcgccg accagacgaa atcaggcttc
       61 gccgcctgga tcttggagat ggtcgagccg aaattggtga cgtcgagcgg gaagaagtcg
      121 atcgagggta cctcgccgcc gttctcggtg acgtatttct tcacccattg cgaggtgatc
      181 tgcccgtaat tgtagtcggc ggcgatgaca tagaccttct tgccccactt cttcatggcg
      241 taaggcacga gcttttcaac cgtttgcgca ggcgtaacgc cggtatcgaa ctggttgcgg
      301 tcgcagacgc cgccttcata ttgcgtgttg tagaaataca gcgtcttgaa gcggtcgagc
      361 accggccgga tcacctcgcg cgaggcggag gtgataccgc catgcacgac ggcaaccttg
      421 tccttcagcg ccgcctgctg cgcgaactgc gtatagagct gcatgttcga ttgggtgtcg
      481 tagttgatca gcttgatctg ccgtccgagc agaccgccgg ccgcattggc ctcctcgacc
      541 gcaagcgtaa tcgcatcgac catcggcttg ccgtagatgt cgagaccacc cgagagatcg
      601 tggatgctgg ccacgttgat cgggtcttcc gcccaggcgc tggaaccgaa cgcgccggtg
      661 gcggcggccg cggccgtgcc ctgtaggaat cttctgcgat tgactttcat tgccgtactc
      721 ctcgttgttg ccgatacgtc atgagaccag atattttttc agaatcgacg cgcccatcgt
      781 gtattttgga tcgaccatgt ttccgagccg gatgcgaccg gcctgactga gcagcatgta
      841 ggcgtcgatc tcgtcgaagc cgtagtccgc gctcatccag cgcaccagtt cgcgataggc
      901 gatgcgggcc gcgtcttcca gcggccgggc gctgccgatc gtcatgagaa agttttcggt
      961 ctcaagccgc ggccacgcaa aactccagtt cttgatgaca tcgacctgca aggtgacggt
     1021 ggcgcgctgt tcgatcgcaa cccccgacaa ttcgccatcg ccctgggtgg catggcaatc
     1081 gccaacatag agcaggccgc ctttggtgtg caccggaaag taaaggatcg cgccaggagc
     1141 tacgtccggc aaatccatgt tgccgccgtg gtagtccggt tgcagcgagg aaatcgcctc
     1201 gatctcgggt gaaaccccga tggtgccgat gaacggctcg tagggaagga tgatcttgtc
     1261 gctccagcgc acaccgtccc tgtcgacatg cattttccgc acccgctccg gcaacggcgc
     1321 attgagcatg gccgttgacg ccgttccgac gaggccgccg aattccggga tgatgcaggt
     1381 ggtgccggcg ggctgcgcgc ctcgtgtctc cacggcatgg atctggacgg ccaggcaatc
     1441 acccttctcg acgccgctga cggccaccgg gccgcactgc ggattgagaa agggaaaccg
     1501 caatttcgcg gtcggactgt cctgctcgcc ggtgatgacg ccgccgaagg cgtcttcggt
     1561 ctccaccacg acaacgtcgc caggattgat cgtcagtacc ggcctggcat agggcccata
     1621 gacgtaatga aaatctcctt gtgccgcgat cgagagttcg tggcgttttc cggcctcgcc
     1681 tttggcaagt ccgcgctttg ccatgatcga agttttcagc caagaggcgc tggtcatcgg
     1741 catctcctca atcacccgag tgaaggatgg caacggtgcc aatcggtgac gcgttggaac
     1801 gcgtcaccgg cggctactaa acgagcttca tcgagatggc ggccgacgaa ctggaacgcg
     1861 accggaccgc cgctctcggt aaagccgccc gaaagcgtaa tggtcggact tcctgtcatg
     1921 tcaaagggac agctgaagcg taacaggccg gcgatcagct cagggttttc gcccaaggtc
     1981 gccattttgg tgagcgtcgg cgcggcgaat gtctgggccg gcacggcaag aagatcgacc
     2041 ttttcgaaca gcgccttcac cattcccttg aaagcttcgc ggcgcagaac gatcttctga
     2101 tagtccttgc cgga
//
LOCUS       DASWGM010000289         6581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2654146,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000289 DASWGM010000000
VERSION     DASWGM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2654146"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VGA15_08420"
     CDS             <1..941
                     /locus_tag="VGA15_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX9587756.1"
                     /translation="GEWMVVEADESDGTFLKLPADVAIVTNVDPEHLDHFKTFEAVQE
                     AFRNFVENVPFYGFAVMCIDHPVVQSIVGKIEDRRIVTYGENPQADARLIDLVPMGSG
                     SSFKVAFRDRKTEVAHEIADLHLPMAGRHNALNATAAIAVAHELGLSDAMIRKAIAGF
                     GGVKRRFTRTGEWNGVTVIDDYGHHPVEIAAVLRAARESTNGKIVAVVQPHRFTRLQS
                     LFEEFCTCFNDADTVVVAEVYPAGEAPIAGIDRDHFVLGLRAHGHREVIALQKSTDLG
                     RVVHGLAGSGDFVVCLGAGNITQWAYALPGELKALG"
     gene            complement(1010..1345)
                     /locus_tag="VGA15_08425"
     CDS             complement(1010..1345)
                     /locus_tag="VGA15_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9587757.1"
                     /translation="MAGLVPAIHVLQNFGMAGGYVYILTNRPNGILYVGVTNDLVRRV
                     FEHRSGFVDGFTKQYGLKRLVYFERFDDIRDAIQREHNIKHWPRAWKVRKIVAANPDW
                     NDLFETITE"
     gene            1375..2298
                     /gene="murB"
                     /locus_tag="VGA15_08430"
     CDS             1375..2298
                     /gene="murB"
                     /locus_tag="VGA15_08430"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX9587758.1"
                     /translation="MTFPDITPDLKAAMPQLRGRLLANQSLAELTWFRVGGPAQLLFT
                     PSDEDDLAYFLEHLPNEIPVYVVGVGSNLIVRDGGMPGVVIRLGPRGFGETSASRDIV
                     SAGAAALDKRVAEAAAAANIGGLEFFFGIPGTIGGALRMNAGANGSETKDVLVEATGF
                     GRDGRKQIFDNAGMKFIYRNSGVDPSFIFTAARFRGLMAAPEAIRARMNEVQNHRETA
                     QPIREKTGGSTFKNPPGESAWKLIDAAGCRGLRVGDAQVSEMHCNFLINTGAATGHDI
                     ETLGETVRARVKENSGIELHWEIKRIGIERT"
     gene            2298..3284
                     /locus_tag="VGA15_08435"
     CDS             2298..3284
                     /locus_tag="VGA15_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX9587759.1"
                     /translation="MRVTILFGGTNTERLVSVASAQAMHAALPEADLWFWDVSDTVHE
                     VQPQALRSHARPFEDAFKPGNRGIGLEPALDKAMAEERLLVLGLHGGRAENGELQAMC
                     EMRGIPFTGSGSASSHLTFDKVAAKRFASIAGVKSPVGLVLEDIDAALAEYGRLIAKP
                     ARDGSSYGLIFVNAKQDLVAVRNAAKTEDYVIEPFIAGVEATCGVLEQLDGSVRALPP
                     IEIVPAEGGFDYTAKYLLKSTQEICPGRFAPEITAQIMDQAVKAHQAMSCSGYSRTDF
                     IISEKGPIFLETNTLPGLTKASLYPKSLKAQGIEFVDFLQGQIALAKKRTYK"
     gene            3347..3667
                     /locus_tag="VGA15_08440"
                     /pseudo
     CDS             3347..3667
                     /locus_tag="VGA15_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663540.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
     gene            3634..4620
                     /locus_tag="VGA15_08445"
     CDS             3634..4620
                     /locus_tag="VGA15_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX9587760.1"
                     /translation="MDGGGRLARSLRSSRPQTDLKAAAIGAAVLLRERLGHHRAGARA
                     RPEREQHPHRLIALLERYIPHRAGVAATVLMLLGSAGFGIVRGGHVEELSSALSDTRN
                     AIANSAGFRITAVAITGRKQLTQDEVLAIGGVNGRSSLLFLDAAAVREKLKANPWISE
                     ATVLKLYPGHLQIDIVERSAFALWQQGGRLSVISDDGAVLEPYMSQRFISLPLVVGKG
                     ADTRARDFLALLARYPQVTAATKAAILVGERRWNLRLKDGLDIRLPENDVGNALATLS
                     KLDKEDRLFSRDIVAVDMRLPDRLTVQLSEDAAKAREDLFKDKKPKKKAGDA"
     gene            4617..5939
                     /gene="ftsA"
                     /locus_tag="VGA15_08450"
     CDS             4617..5939
                     /gene="ftsA"
                     /locus_tag="VGA15_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX9587761.1"
                     /translation="MTGLDRNQTPKTRPMPQNRTALVASLDIGTSKIACMIARLRPCP
                     PSDALRGRTHAVELIGYSQIQSRGVKAGAVVDLAECEQAVRHAVALAERMAKVRVESV
                     LLSVSGGRLHGQLVEASADIRGGAVTSADVIRVTSTGMRHATGEGRTVLHALPVGYAL
                     DGVKGIRDPRGMVARQFGVDMNVVTVEATVAKNLMLVVERCHLNVEAMAASPYVAGLS
                     VLTDDEADIGAAVVEMGAGTTTIATYAGGRFVHASGFAVGGQHITMDLARGLSACIAD
                     AERIKTLYGTVLTGGSDARELMSVPAAGDNERDAPQIVSRATIANIVRQRAEEIFEMV
                     RDRLADSPFAAEPKARVVLSGGASQLSGTLELATQILGRPVRIGRPLGFGRLPSEAKN
                     ASFAVPSGLLVYPQYAHLEHVEPRHTRQIRTGTDGYFGRVGRWLREGF"
     gene            6030..>6581
                     /locus_tag="VGA15_08455"
     CDS             6030..>6581
                     /locus_tag="VGA15_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX9587762.1"
                     /translation="MALNLTPPDISELKPRITVFGVGGAGGNAVNNMITAGLQGVDFV
                     VANTDAQALTMSKAQRIVQMGTQVTQGLGAGSQPDVGAAAAQEVIDELRDHLSGANMV
                     FVTAGMGGGTGTGAAPVIAKTARDMGILTVGVVTKPFHFEGQRRMRTAEAGIAELHKV
                     VDTLLIIPNQNLFRVANEKTTFAD"
BASE COUNT         1246 a         2008 c         2108 g         1219 t
ORIGIN      
        1 ccggcgaatg gatggtggtg gaggccgacg agagcgacgg aacgtttttg aagctgccag
       61 ccgacgtcgc catcgtcacc aatgtcgacc ccgaacatct cgatcatttc aagacttttg
      121 aggcggtgca ggaagcgttc cgcaatttcg tcgagaacgt gccgttctac ggttttgccg
      181 tgatgtgcat cgaccatccg gtggtgcaga gcatcgtcgg caagatcgag gaccgccgca
      241 tcgtcaccta tggcgaaaat ccgcaggccg acgcgcgcct gatcgatctg gttccgatgg
      301 gcagcggttc cagcttcaag gtcgcatttc gcgatcgcaa gaccgaggtg gcgcacgaga
      361 tcgccgatct ccacctgccg atggcgggac ggcacaacgc gctcaacgcc accgcggcga
      421 tcgcggtcgc ccatgaactc ggactgtccg acgcgatgat ccgcaaggcg atcgcgggtt
      481 tcggcggcgt caagcggcgc ttcacccgga ccggcgaatg gaacggcgtc accgtcatcg
      541 acgactacgg ccatcacccg gtcgagatcg cggcggtgct gagggcggcg cgggaatcca
      601 ccaacggcaa aatcgtcgcc gtggtgcagc cgcaccgctt cactcggttg cagtcgctgt
      661 tcgaggaatt ttgtacctgt tttaacgatg ccgatacggt tgtcgtcgcg gaggtttatc
      721 cggccggcga agcgccgatt gccgggatcg accgcgacca tttcgtgctg ggcttgcgcg
      781 cgcacggcca tcgcgaggtg attgcgctgc agaaatccac cgacctcggt cgtgtggtgc
      841 acggcctcgc cggcagcggc gattttgtgg tctgcctcgg cgctggcaac atcacgcaat
      901 gggcctacgc gctgccgggc gaattgaagg cgctggggtg agcgcggcga tgcaacgggc
      961 gccgtattta ggttcgtcat ggccgggctt gtcccggcca tccacgtctt tactccgtaa
     1021 ttgtctcgaa gagatcgttc caatccggat ttgccgcgac gatctttcga accttccatg
     1081 cacgcggcca gtgtttaata ttgtgctccc gctggatcgc gtcgcgaatg tcatcgaacc
     1141 tctcgaaata aaccagtcgc ttcaatccat attgcttggt gaagccatca acaaatccag
     1201 agcgatgctc gaatacccgg cggacgagat cgtttgtcac gccgacatac aggatgccgt
     1261 tggggcgatt ggtcagaatg taaacgtagc cacctgccat gccgaaattc tgcaagacgt
     1321 ggatggccgg gacaagcccg gccatgacga aattaattgc tgtaacaggt acgcatgacc
     1381 ttccccgaca tcacgcccga tctcaaagcc gcgatgccgc aactgcgcgg acggctgctg
     1441 gcgaaccagt cgctggccga gctgacgtgg tttcgggtcg gcggtccggc gcagctgctg
     1501 ttcacgccat cggatgaaga tgacctcgcc tatttccttg agcatctgcc gaatgagatt
     1561 ccggtctacg tcgtcggcgt tggctccaat ctgatcgtac gcgacggtgg catgccgggc
     1621 gtcgtgatcc ggcttggccc gcgtggtttc ggcgaaacca gcgctagtcg cgatatcgtc
     1681 agcgccggtg cggctgcgct cgacaagcgc gtcgccgagg cggcggcggc ggccaatatc
     1741 ggcgggctgg aatttttctt cggcattccc ggcaccatcg gcggcgcgct gcgcatgaac
     1801 gccggcgcca acggttcgga gaccaaggat gtgctggtcg aagcgaccgg ttttggccgc
     1861 gacggcagaa aacaaatatt cgacaatgcc gggatgaagt tcatctatcg caatagcggc
     1921 gtcgatccct cgttcatctt tacggccgcg cgctttcgcg ggctgatggc cgcgccggag
     1981 gcgatccgcg cacgcatgaa cgaggtgcag aaccaccgcg aaaccgctca gccgattcgc
     2041 gaaaagaccg gcggctcgac cttcaagaat ccgccgggcg aaagcgcctg gaagctgatc
     2101 gatgccgccg gctgccgcgg cctacgggtc ggcgacgcgc aggtatcgga aatgcactgc
     2161 aacttcctga tcaataccgg cgccgcaacc ggccatgata tcgagaccct gggggagacc
     2221 gttcgtgcaa gggtgaaaga gaattcggga attgagctac actgggaaat caagcggatt
     2281 gggatcgagc gaacctgatg cgggttacca ttcttttcgg cggtaccaac acggaacgtc
     2341 tcgtgtcggt agcttccgcc caggcgatgc acgcggctct gccagaggcg gatctgtggt
     2401 tctgggacgt cagtgacacg gtgcatgagg ttcagcctca ggcgcttcgc agtcatgcaa
     2461 ggccgttcga ggacgctttc aaacccggca atcgcggcat cggactagaa ccggcgcttg
     2521 ataaggcaat ggcggaggag cgcttgctgg tgctcggcct gcacgggggt agggccgaga
     2581 atggcgagct tcaggcgatg tgcgagatgc gcgggattcc ctttaccggc tcaggctcgg
     2641 cgtcctcgca tcttaccttc gacaaggtcg cggccaagcg ctttgcgtct atcgccggcg
     2701 tgaagtcgcc cgtcggcctg gtgctggaag atatcgatgc cgcgctggcc gaatacggcc
     2761 gactgattgc caagccggca cgggacggat cgagctacgg cctgatcttt gtcaatgcca
     2821 agcaggacct cgtcgccgtc cgcaacgcgg ccaagaccga agattatgtg attgaaccat
     2881 tcatcgcagg cgtggaagcg acctgcggcg tgctggagca attggacggc tcggtcaggg
     2941 cgctcccgcc aatcgaaatc gtcccggcgg agggtgggtt cgactacacc gccaagtatt
     3001 tgctcaaatc gacgcaggag atttgcccgg ggcggtttgc acctgaaatc accgcgcaaa
     3061 tcatggatca ggcggtcaag gcgcaccagg cgatgtcgtg cagcggctat tcccgcaccg
     3121 acttcatcat ctcggagaag ggaccgattt ttctcgaaac caacaccttg ccgggtctga
     3181 ccaaggcttc gctctatccg aagtcgctca aggctcaggg aatcgagttc gttgacttcc
     3241 tgcaaggtca gatcgcgctg gcgaagaagc gcacgtataa gtagtaaacg gttgagaaat
     3301 acgttggcaa tccggtcggg gtgatcgaaa tccttaactt tcggtaaaat tccaggattc
     3361 tggggcaaag tactcaaaag gcggatcaaa tcacagccag cctcaaccta atttaccatt
     3421 tgtttaccag gaagcccgaa ggttaacgct ggcggcgcat ttggcgcttg gctgccgggg
     3481 cgtggagccg tgcggatttc cgtgtcgacg cccgcatcga gggaacaggt ggcctctttt
     3541 gccttgaggt cgagacccag cccggcatga ccaggacgtc acttgtgcca gaactcgcag
     3601 tgtttgcggg cacacatttg acgagcttgt gcaatggatg gtggaggacg cctcgcccga
     3661 tcgttgagat cgtcgaggcc ccaaactgac ctgaaagcgg ccgccattgg cgcggccgtg
     3721 ctgttgcgcg agcggctggg ccatcatcgc gccggcgcca gggcgcgccc ggaacgcgaa
     3781 cagcatccgc atcgcctgat cgcgctgctt gagcgttaca ttccgcaccg cgccggcgtt
     3841 gccgcgaccg tcttgatgct gcttggcagc gccggtttcg gcatcgtgag gggcggccat
     3901 gtcgaggaat tgtcctcggc gctcagcgac acccgcaatg cgatcgcaaa ttccgcggga
     3961 tttcggatca cggcggtcgc catcacaggc cgcaagcaat tgacccagga cgaggtcctc
     4021 gccatcggcg gcgtcaacgg ccgctcttcg ctgctgttcc tcgacgccgc ggccgtgcgc
     4081 gaaaagctga aagccaatcc gtggatcagc gaagccacgg tgctgaagct ttatcccggc
     4141 cacctccaga tcgatatcgt cgagcgttcg gcattcgcgt tatggcagca gggtggccgg
     4201 ctgtcggtga tttcagatga tggcgcggtg ctcgagccct acatgtcgca gcgttttatc
     4261 tcgctgccgc tggtcgtcgg caagggcgcc gatacccgcg cgagggattt cctcgcgctg
     4321 ctcgcgcgct acccgcaggt aaccgcagcg accaaggccg cgattttggt gggcgaacgg
     4381 cgctggaacc tgcggctcaa ggacggtctg gatatccggc tgccggagaa cgatgtcggc
     4441 aacgcgctgg cgacgctgag caagctcgac aaggaagaca ggttgttctc gcgcgatatc
     4501 gtcgccgtcg acatgcgtct gccggaccgg ctgaccgtgc aattatcgga agacgcggcc
     4561 aaggcccgcg aagatctgtt caaggacaag aagccgaaga aaaaggccgg tgacgcatga
     4621 ccggcctcga tcgcaatcaa acgccgaaga cgcgtccgat gccgcagaac cgcaccgcgc
     4681 tggtggcgtc gctcgatatc ggcaccagca agatcgcctg catgatcgcg cggctccggc
     4741 cgtgcccgcc gagcgacgcc ttgcgcggcc gtacccacgc cgtcgagttg atcggataca
     4801 gccagatcca gtcgcgcggc gtgaaggccg gcgcggtggt cgaccttgcc gaatgcgagc
     4861 aggcggtgcg tcatgcggtc gcgctggccg agcgcatggc caaggtccgg gttgagtccg
     4921 tgctgctctc ggtatcgggt ggccggctgc acggtcaatt ggtcgaagcc tcggcagata
     4981 ttcgtggcgg cgcggtcaca tcagccgacg tcatccgcgt cacctctacc gggatgcgcc
     5041 acgccaccgg ggagggccgt accgtgctgc acgcgctgcc ggtcggctac gcgctggacg
     5101 gcgtcaaagg cattcgggac ccgcgcggca tggtggcgcg gcaattcggc gtcgacatga
     5161 acgtcgttac cgtagaggcc accgtcgcca aaaacctgat gctggtggtg gaacgttgtc
     5221 atctcaatgt cgaagccatg gcggcgagcc cctatgtggc gggcctgtcg gtgctgaccg
     5281 acgatgaggc cgatatcggc gccgccgtgg tcgaaatggg cgctggaacc accacgattg
     5341 cgacctatgc gggcggccgg ttcgtccatg ccagcggatt tgcggtcggc ggccagcaca
     5401 tcaccatgga tcttgcacgg ggcttaagcg catgcattgc ggatgccgag cgaatcaaga
     5461 cgttatatgg caccgtgctg accggcgggt ccgacgcgcg tgagttaatg tctgtgccgg
     5521 ctgccggtga caacgagcgg gatgcgccgc agatcgtgtc ccgagccacg attgcgaaca
     5581 ttgtccgaca acgcgccgag gagatttttg aaatggtcag ggaccggctc gcggattcgc
     5641 cctttgcggc agaaccgaag gcgcgggtgg tcttgagcgg cggcgcttcg cagctctccg
     5701 gcaccctcga acttgcgacc cagattctcg gccggccggt gcgcatcggc cgtccgctcg
     5761 gctttggccg gctgcccagc gaggcgaaga acgcgtcgtt cgcggttccc tcgggtctgc
     5821 tggtttatcc gcaatacgcg catcttgaac acgtcgaacc gcgacatacg cggcagatca
     5881 ggacagggac tgacggctat ttcggaaggg tcggacgatg gcttcgcgag ggcttctgat
     5941 gaatcttttc cgacccattc gacttccacc agctcccgcg gccgtcggcc gaggcgaacc
     6001 aacgcgcgcg tcgtaatcga gaggcaacca tggcactcaa tcttaccccc cctgacatca
     6061 gcgagttgaa gccgcggatt accgtattcg gagtcggcgg agctggcggt aatgccgtca
     6121 acaacatgat caccgccgga ctacagggtg tcgatttcgt cgtcgccaac accgacgcgc
     6181 aggcgctcac catgtccaag gcccagcgca tcgtgcagat gggcacccag gtcacgcagg
     6241 gcctcggcgc cggttcgcag ccggatgtcg gcgcggccgc ggcccaggaa gtcatcgacg
     6301 agctccgcga ccatctctcc ggcgccaaca tggtgttcgt caccgccggc atgggtggcg
     6361 gcaccggcac cggtgccgca ccggtcatcg ccaagaccgc tcgcgacatg ggcatcctca
     6421 cggtcggcgt ggtgacaaaa ccgttccact tcgaaggcca gcgccgcatg cgcaccgccg
     6481 aggccggcat cgccgagctc cacaaggtgg tcgatacgct gctgatcatt ccaaaccaga
     6541 acctgttccg ggtcgccaac gaaaagacca ccttcgccga c
//
LOCUS       DASWGM010000290         6823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_297668,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000290 DASWGM010000000
VERSION     DASWGM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297668"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VGA15_08460"
     CDS             complement(<1..447)
                     /locus_tag="VGA15_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03808 family TAT-translocated repetitive
                     protein"
                     /protein_id="HEX9587763.1"
                     /translation="MDINRRHLIGASAAGVAGALAMSTDDARAAPLTSALGRDATHYG
                     VRPGSPDDQTQILQRAIDDAAGAQVPLALPPGVYRTGMLRLQSGTQLVGVRGATRFVF
                     AGGASMFQGEGTNSVGLSNLTLDGAGIALPVRRGLVHCLGGRDIRVT"
     gene            complement(604..1272)
                     /locus_tag="VGA15_08465"
     CDS             complement(604..1272)
                     /locus_tag="VGA15_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEX9587764.1"
                     /translation="MTDKLRILITGFGPFPGAPFNPTQPLVARLTRLRRPAFDDVELI
                     GHIFHVTYNTVDRELPELLGKHRPQALLMFGLADRTPHVRIETRARNAVTTLFPDADR
                     NRARKGSIVGGADAMMLGPHTAKLLRAAVGTGIDARASRDAGSYLCNYLSWRAIEAVN
                     SGDGARLAAFVHVPLLARGGASQRKDAAHRITLEDLVDAGEAMLLELVKLARQAVRDR
                     DTSE"
     gene            complement(1274..2263)
                     /gene="meaB"
                     /locus_tag="VGA15_08470"
     CDS             complement(1274..2263)
                     /gene="meaB"
                     /locus_tag="VGA15_08470"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX9587765.1"
                     /translation="MPLPNAPIDIIALTKDLRAGSRAALARAITLIESRRSDHQAAAR
                     ELVQALLPDTGKAIRVGITGSPGVGKSTTIDVLGMFLIERGDKVAVLAVDPSSARTGG
                     SILGDKTRMARLSASDRAYIRPSPASGTLGGVAAKTREAMLLCEAAGFDVVLVETVGI
                     GQSETAVCDMTDFFLALMLPGAGDELQGIKKGLVELADMIAVNKADGDNIKRANLAAA
                     EYRGALHILTPRSEHWHPPVLTYSALTGKAIGELWQKIIDHRAAMNASGEFAARRRQQ
                     QVKWMWSMLEQRMLARLRADPAIRADVKKIEAEVADGRITPALAAEQIAEMLK"
     gene            complement(3067..4110)
                     /locus_tag="VGA15_08475"
     CDS             complement(3067..4110)
                     /locus_tag="VGA15_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587766.1"
                     /translation="MRATVIAGQRYADDYAVIWRGMWIGRILRGAGAPHGTPPWSWSC
                     HLPGRPQGNDDRGNAVDLDDAKQQFKAAWARIRAGLSEADIAGGSPDCRDQRRGAREI
                     RPEVQSLSFSRVGTMQDRYAGDVGDYVKFALLRHLGPGRRWGIAWYLHPDTKDGGHTK
                     YLREPERWRELDHKLFDLLSAIEQRQRSVAALQAASIITANFSNDRLDCSAVDIRERD
                     IWRSKWFAEVLRKLSACDLVFADPDNGLVDDRQDRRRRAVFCKQIPLAEAKALAVGRT
                     AVIYHHNTRRKGGHLAEIGHWMEQLGTGTIAVRANAYTCRTFFILNPDHEIRNRAISF
                     CHRWKEHLVSFVG"
     gene            complement(4145..4351)
                     /locus_tag="VGA15_08480"
     CDS             complement(4145..4351)
                     /locus_tag="VGA15_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587767.1"
                     /translation="MADTVRVFDFEIAGIHGARVVAVKEPLTPGCCDDGEIDANIELL
                     KKDLDAVAVRMKAAVRKQAAQPDF"
     gene            4701..5108
                     /locus_tag="VGA15_08485"
     CDS             4701..5108
                     /locus_tag="VGA15_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587768.1"
                     /translation="MPVATDLTILLVALTNENQIKLSCGRRDQSRNQPTWLCPAGRSS
                     VFAKAPPTSKPNSRSFDVFAPFKYLTAIAAIFLILFGTLPVFVSALYGGNPPPHVARL
                     LDKQMEVFATVAPIIFAPFKAFASNEKPPDDKK"
     gene            complement(5202..5369)
                     /locus_tag="VGA15_08490"
     CDS             complement(5202..5369)
                     /locus_tag="VGA15_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587769.1"
                     /translation="MIRAPKEFRDNEYSKDEAKGRFESALNYGDSALNSIWPFHGVAR
                     VTVLNEDPSSS"
     gene            5368..6123
                     /locus_tag="VGA15_08495"
     CDS             5368..6123
                     /locus_tag="VGA15_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587770.1"
                     /translation="MGTIPLEDAFYSAKRSYERGQYHIQDFDTRLAEFFNTEPWLAVT
                     EIDTDRKQFHKFRWTKKPPEALESIATDAAYNFRAALDQCGYTAAVLFGKNDATHAHF
                     PFGNNGREGVTIPRKSRGRDLPPEILAVFRSFKPYPGGNHALNTLNELCNTNKHSFLV
                     TAEFRDEWAALSDVEGIDPYWDRGKQQIIFSSTDGNTRHHVSVAHQIVFQGIPNLEGR
                     PVVRNLDLMRDVVLQVISETQETCFRCGKLIRR"
     gene            complement(6374..6688)
                     /locus_tag="VGA15_08500"
                     /pseudo
     CDS             complement(6374..6688)
                     /locus_tag="VGA15_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1304 a         2100 c         2061 g         1358 t
ORIGIN      
        1 ggtaacgcgg atgtcgcgtc cgccgaggca atgcaccagg ccgcgccgca ccggcagagc
       61 aatgccagcg ccatccagcg tgaggttgga aagaccaacg ctgttggtgc cttcgccctg
      121 aaacatcgac gcgccgccgg cgaacacgaa cctggtcgcg ccgcgcacgc cgaccaactg
      181 ggtgccgctc tgcaagcgaa gcatcccggt gcgatagacg cccggcggca gcgccagcgg
      241 cacttgcgcg ccggcggcat cgtcgatcgc ccgctgcagg atttgggtct ggtcgtcggg
      301 actgccgggc cggacgccat aatgcgtggc gtcgcgtccg agcgccgagg ttagcggcgc
      361 tgcccgcgcg tcgtcagttg acatcgccag cgcgccggca acgccggccg cggaagcccc
      421 gatgagatgg cggcgattga tgtccatgat cgcgtcttcc tgtcgatacg ctggaggcgt
      481 ttccgcgacc tctgagtaac gcaaaaatgc ggcggagcgt ggcaatggca agggcacaag
      541 agggcgatag tcaggagtag ggttaatcct gacctcgccc cgcttgcgcg gagagcgaga
      601 agatcactcc gacgtgtcgc gatcccgcac cgcttgcctt gccagcttca ccaattccag
      661 cagcatggct tcgccggcat cgaccaggtc ctccagcgtg atccggtgcg ccgcgtcctt
      721 gcgctgcgat gcaccgccgc gcgccagcag cggcacatgc acgaaggcgg cgaggcgagc
      781 accatcgccg ctgttcacgg cctcgatcgc ccgccagctc agatagttgc agagatagct
      841 tccggcatcg cgcgaggcgc gtgcgtcgat gcccgtgccc acggccgcgc gcaacaattt
      901 ggcggtgtgc gggccgagca tcatggcatc cgcgccgcca acaatcgatc ctttccgggc
      961 gcggttgcgg tcggcatcgg gaaacaacgt cgtcacggca ttgcgggcgc gggtttcgat
     1021 ccggacatgg ggtgtgcggt cggctagacc gaacatcaag agcgcctgcg ggcgatgttt
     1081 ccccaacagc tcaggcagtt cgcggtcgac cgtgttgtag gtgacatgaa aaatgtgacc
     1141 gatcagttcg acgtcatcga atgcgggacg gcgcagccgc gtcagccgtg ccaccagcgg
     1201 ctgcgtcgga ttgaagggcg cgccgggaaa cgggccgaag ccggtgatga gaatgcggag
     1261 tttgtcggtc atttcacttc agcatctccg cgatctgctc cgccgccagc gccggcgtga
     1321 tgcggccatc ggccacctcg gcttcgatct tcttcacgtc tgcgcgaatg gcaggatcgg
     1381 cgcgcagccg cgccagcatc cgttgctcca gcatcgacca catccacttt acctgctgct
     1441 gccgccgccg cgcggcaaat tcacctgacg cgttcatcgc ggcgcggtgg tcgataatct
     1501 tctgccagag ctcaccgatg gctttgcccg tcagcgccga ataggtcagg accggcggat
     1561 gccagtgctc cgagcggggg gtgaggatgt gcagcgcgcc gcgatactcg gcggcggcca
     1621 gattggcgcg cttgatattg tcaccgtcgg ccttgttgac cgcgatcatg tcggccaatt
     1681 cgaccaggcc cttcttgatg ccttgaagct cgtcgccggc gcccggcagc atcagcgcca
     1741 ggaagaaatc cgtcatgtcg cagaccgcgg tctcggattg cccgatcccg acggtctcca
     1801 ccagcaccac gtcgaagccg gcggcctcgc acaacagcat cgcctcgcgg gttttggcgg
     1861 cgacgccacc gagcgtgccg gatgccggcg agggccggat gtaggcacga tcggaggcgg
     1921 agagccgcgc catccgcgtc ttgtcgccga ggatcgagcc gccggtgcgc gcggaagagg
     1981 ggtccaccgc gagcaccgcg accttgtcgc cgcgttcgat caggaacatc ccgagtacgt
     2041 cgatggtggt ggatttgccg acgccgggcg agccggtgat gccgacccgg atcgccttgc
     2101 cggtgtcggg cagcagcgcc tgcaccagct cgcgcgccgc ggcctgatga tcgctacgcc
     2161 tgctttcaat cagcgtgatc gcccgcgcca gcgccgcccg gctgccggcg cgaaggtctt
     2221 ttgtcagggc gatgatgtcg atgggggcgt tcggcagggg catgtcttgc tttacaacgg
     2281 cgacgcgtgg cacgcgaggg ggccggactg ccgacgcgcg ccgaacagcc ggtttcagat
     2341 gcaatttcgc gttcccgcgg cgcagcgcgc ccgagttgtg catggtcctt ttccgcccta
     2401 aaaaacagag ggcgtgggga atgccgggtg cccgccgcac ccgcggcctc gtgtgcgctt
     2461 tgaattggta aaaagcacac gagtatacga gtacaccgga atcacccggc attcccgcac
     2521 gcaatggttt taacggttta tgtcgtactc tccccggtga ccgggctctt ttgccaccgt
     2581 cgcctgcgga tatgttttgt ctaagcccgg ttgggccgac gcaactccgc agacttgaca
     2641 ccagcgtcgg ggtgtcagga ccacacgatt ttgccgtacg ctgcagcatc tctcgtccgc
     2701 tcgcttggtg atcactcacg ggcctcatcg acccgccctg cgatcccgtc gcgcgcaaaa
     2761 cgctgccgcg tccaccgcat cccatcccgc gtccgtgacg atcgcgatac gcccctgtgg
     2821 tggggtggga tgcgaaaagt tctagatgtg atttggggtg cgtgaaaaca gaaatatttt
     2881 tgcggaaggg actcgacaca aaactgcccg acgggcaaat caccgcactg cgtgcactcc
     2941 attcgcgtcc cggacgcggt gcagcgtact tcacgccgct ccgcagagcc ggggcccacc
     3001 gccgggcgag cggcgcgatg aatcccggat gtcgcttcgc tcatccgggc tacgcttgct
     3061 gtcccgtcag ccgacgaagg aaactaaatg ttccttccaa cgatggcaga acgagatcgc
     3121 ccgattgcga atttcatggt ctggattgag tatgaaaaaa gtccggcacg tataggcgtt
     3181 tgctctgacc gcaattgtgc ccgtgccgag ctgttccatc cagtgtccga tttccgcaag
     3241 atggccgcct ttccgacggg tattgtgatg gtagattacg gcggttcttc ccacggccaa
     3301 cgccttcgcc tcagcgaggg gaatttgttt gcagaaaacg gctctacgcc gccgatcctg
     3361 ccgatcatcg acgaggccgt tgtcggggtc agcgaatacg agatcgcaag ccgacagctt
     3421 ccgcaatacc tcagcgaacc atttgcttcg ccagatatcg cgctctcgta tatcaacggc
     3481 tgagcaatcc aagcgatcat ttgaaaagtt ggccgtgatg attgaggcag cttgcagggc
     3541 tgcgacagaa cgctgtcgct gctcgattgc ggacaacaga tcaaataatt tgtgatcgag
     3601 ctcgcgccag cgttccggct ctcgcaagta tttggtgtgc ccgccgtctt tagtatctgg
     3661 gtggagatac cacgcgattc cccaacggcg gccgggaccg agatggcgga gcaaagcaaa
     3721 tttgacgtaa tcaccaacgt ctcccgcgta tcgatcctgc attgtcccaa cccttgagaa
     3781 actcaagctc tgcacttccg gtcgtatctc gcgagcgcct cggcgctgat ctcggcaatc
     3841 cggtgagcct cccgcgatgt cggcttcgga caggcccgcg cggatgcgcg cccatgccgc
     3901 cttgaattgc tgcttcgcgt cgtcgaggtc gacggcgttg ccccggtcgt cgttgccctg
     3961 cggcctgccg ggcaggtgac acgaccacga ccatggcggc gttccatgcg gcgcgccggc
     4021 accgcgcaga atgcggccta tccacatgcc gcgccagatc accgcgtaat cgtcggcgta
     4081 gcgctggccg gcgatgacgg tcgcgcgcat ggtgagggat tcgtcgggca tgccccttgc
     4141 aattctaaaa gtccggttgc gcggcctgct tgcggaccgc tgctttcatt ctaaccgcga
     4201 ctgcatcaag gtcctttttc agaagctcga tgttggcatc gatttcgccg tcgtcgcaac
     4261 agcccggcgt caacggttct ttcaccgcga cgactcgtgc gccgtggata ccggcgatct
     4321 cgaaatcgaa tacgcgaaca gtatcggcca tttttgcctc cgtgaggtta gagttgatcg
     4381 ggtcttcgcg ctattaatta ccgcctgttc tttcgcacct gttctacgac gtctaccgct
     4441 cgcttgaact cgtcgaggtc gatctctgga tcgtttggtg atagcgcgta gggcggattg
     4501 agccggaggc gtaatccgcc attccggcgg gcgtaacggc ggatcacgct gcgctgatcc
     4561 gtcctaggcg ctgtcaccgc gattcccccc agcgcgggga tttttttgcc attgaattgc
     4621 catgaactgc catcacctct taggcacaac ggcaagccga gcgcatcggc ggccgttact
     4681 ccccgcaagg aagtgcgggg atgcccgtcg cgacagattt gactatactg ttggtcgcgc
     4741 tgaccaacga aaatcaaata aagctctcgt gcggtcgacg ggaccaatcg aggaaccaac
     4801 caacctggct ttgcccggcg ggtcgttcct cggtatttgc caaggcaccc cccacctcaa
     4861 agccaaacag tcggagcttc gacgtgtttg cgccattcaa atatctaacc gccatcgcgg
     4921 cgatttttct cattctcttc ggcacgcttc ccgtttttgt cagcgctctc tacggcggca
     4981 acccgccgcc gcatgttgcg cgcttgctcg acaagcagat ggaggtgttc gccacggtcg
     5041 cgccgattat cttcgcgcct ttcaaagcgt tcgccagcaa tgagaaacca cccgacgaca
     5101 agaagtagca tggcgtcatg ctgacagctt gtacacgctt ggcagcgaag gcagatggct
     5161 ggtcaactgg gaagttatgg ggaattacgg tgacagtgca cttaacttga tgaaggatct
     5221 tcgttcagca cggtcacccg cgccacacca tggaacggcc aaatcgaatt aagtgcactg
     5281 tcaccgtaat tcaatgctga ttcaaagcga cctttcgctt cgtctttaga atattcgttg
     5341 tcacgaaatt ctttgggagc acgaatcatg ggaacgatcc cgttggagga cgccttctat
     5401 agcgcgaaac ggagctatga gagagggcaa taccacatcc aagattttga tacgcggctc
     5461 gccgaatttt tcaacacgga gccatggctc gccgtcaccg aaatagacac tgacagaaag
     5521 cagtttcata agttcagatg gactaagaag ccacccgaag ctctggagag catcgccact
     5581 gatgctgctt acaactttcg cgctgccctc gatcaatgtg gatatacggc cgcagtcctt
     5641 ttcgggaaga acgacgccac acacgcccac ttcccgtttg ggaataatgg tcgggaaggc
     5701 gtcaccattc cccgcaagtc ccggggacgg gacttgccgc ctgaaatcct cgcggttttt
     5761 cgatcgttca agccataccc aggagggaac catgccctca acacactgaa tgagctttgt
     5821 aatacgaaca aacacagctt tctcgtcaca gcagaattta gggacgaatg ggccgctctc
     5881 agcgacgttg aaggtatcga tccctattgg gatcgcggca agcaacaaat catattctcc
     5941 agcaccgatg gtaatacgcg ccatcacgta agcgtcgccc accaaatagt gtttcagggg
     6001 attcccaacc tcgaaggacg cccagtagtt cgcaatctcg acctgatgcg agatgtcgtc
     6061 ttgcaagtca tctccgaaac tcaagagaca tgtttccgct gcgggaaact tatccgccgc
     6121 taattcggac gcgccagctt cgtccgatag ggccgaagcg gagcatttac tatctgtcat
     6181 cccgctctat gtttttgttt gagcatgatc tccgcgcaaa cgcttcgcgt ttgtcgcgag
     6241 ggaaaaccgg tacccacctt tccggatcgt gctctagaaa ttacggtgac agtgcactta
     6301 acgtaagcat ccgttaaagg ccgcagggtc agcggctcgg ttgatggcgg tcgcggacgc
     6361 gcggcgcgca tgatcaggcg gcgcgattga gttgtgcggg acgccagttc cagggcagca
     6421 gcgcggcgag atcgttgatc ttgtgatcgg cgatgcgcgt cagcacgtcg gcgagccagg
     6481 cttgcggatc gacgccgttg agcttggcgg tctggatcag cgtgagcatg accgcggcac
     6541 gttcgccgcc gcgatcggag ccggcgaaca gccatgcctt gcggccgagt gcgatgccgc
     6601 gcaacgcgcg ctcggctgcg ttgttgctca ggcagatgcg cccatcggcc aggaagcggg
     6661 taaacgcgtc actgcgtttg agcatgtaat accagacttc ggattgatta acctatggag
     6721 gcccagtcgc ggctggcaat ggacgcgatg cggccgagtt cgttgagaag gctacgccaa
     6781 gcgttctgac acgcgtcgag gattgcggtg taggtttcga aca
//
LOCUS       DASWGM010000291         2535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1133504,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000291 DASWGM010000000
VERSION     DASWGM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133504"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGA15_08505"
     CDS             <1..325
                     /locus_tag="VGA15_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9587771.1"
                     /translation="RGAIAHLKNAKVTGYQTYDEIFGLLKSGEVDAFALSRDQLNAMA
                     KKIPGTRVLDETFKQTVTAVAVPPNHPLALAFVTRFMTDAPSNGTLRKAYDNNGLKDN
                     PIRVK"
     gene            complement(428..1786)
                     /locus_tag="VGA15_08510"
     CDS             complement(428..1786)
                     /locus_tag="VGA15_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX9587772.1"
                     /translation="MSNFRQIDRATGFLLPPSVDEWLPERHLARFIVEVIAGLDLRSM
                     SGSYRGSGSASYHPEVLLGLLVYGYATGVFSSRKLERATYDSVAFRFIAANDHPDHDT
                     IATFRRRFLPQIAGLFLQVLVLAREIGVLKLGTIGLDGTKIHANASRHSALSYEHAGN
                     IETQLKAEVADLMAKAEAADQADIPDGFSIPEELERREERLRKLAEARAKIEARAKER
                     HAREQAEHEAKLAARKAREESSGKKTGGRPPSPPASGALPTDQINLTDEESRIMPVAG
                     GGFEQCYNAQAVVTAGSMLVVAVHVVQAPNDKQQLAPMLDQISALPKELGQAETLLAD
                     SGYFSEANVEACVAAGIEPLIPQGRQAHYPPLSERFANVPPAPDNSTPLETMAHRLKT
                     PEGKNLYALRKQTPEPVFGIIKSVMGFRQFSMRGLEKVRGEWSLVTMAWNIKRMFTLM
                     PA"
     gene            complement(1867..>2535)
                     /locus_tag="VGA15_08515"
     CDS             complement(1867..>2535)
                     /locus_tag="VGA15_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9587773.1"
                     /translation="ELVPEFERLTGNTVLTAYGPSMGTTVNAIPIRLERGEPADVLIM
                     VGYALGELVKQGKVVADSRVDLVKSPIGIAVKSGAPKPDISSADAVKRALLATKTIAY
                     SDSASGVYVSTEMFEKLGIADAMKDKARKIPATPVGEIVARGDAEIGFQQVSELLPVQ
                     GIDIVGQLPSELQKVTVFSAGIASVSKEPDAGKALIKFLASPMASAVIVKSGMEPIPA
                     GATN"
BASE COUNT          478 a          824 c          740 g          493 t
ORIGIN      
        1 gcgcggcgcg atcgcgcatc tcaagaacgc caaggtcacg ggctatcaga cctatgacga
       61 aatcttcggc ctgttgaaga gcggcgaggt cgacgcgttc gcgctgtcgc gcgaccagct
      121 caacgccatg gcgaaaaaga ttccgggaac gcgcgtgctg gatgaaacct tcaagcagac
      181 cgtgaccgcg gtggcggtgc cgccaaatca tccgctcgcg ctggcattcg tgaccaggtt
      241 catgaccgac gctccgtcga acggcacgct gcgcaaggcc tacgacaaca acggcctgaa
      301 ggataatccg atccgggtga aatagcggag cttttaattc cgctagcagc ctgtcggact
      361 gaggggggcg gaggcgattt tggggctttt gaagccagcc tttggctctg tgccggctga
      421 gcagctctca ggcaggcatc aaggtgaaca tgcgcttgat attccatgcc atggttacga
      481 ggctccattc gccacggacc ttttcgaggc cgcgcatcga gaactgtcgg aagcccatca
      541 ccgatttgat gatgccgaac accggctccg gggtctgctt gcgcaacgcg tagagatttt
      601 tgccttcggg cgttttcagc cgatgcgcca tggtctcgag cggcgtcgaa ttgtccggag
      661 cgggtggcac atttgcaaag cgttcgctga gcggcgggta atgcgcctgc cggccctgcg
      721 ggatcagcgg ctcgatgccc gccgcgacgc aggcttccac attggcttcg ctgaagtagc
      781 cgctgtcggc cagcagtgtt tcggcctggc ccaattcctt cggcaaagcc gatatctggt
      841 cgagcatcgg cgcaagctgc tgcttgtcgt tgggcgcctg caccacgtgg accgcaacca
      901 ccaacatgct gcctgcggtg actacggcct gcgcattata gcattgctcg aagccgccgc
      961 cggccaccgg catgatccgc gattcctcgt cggtcagatt gatctggtcg gtgggcaaag
     1021 cgccggatgc aggcggtgac ggcggccggc cgcccgtctt tttgccgctg ctctcctccc
     1081 tggccttgcg cgctgcaagc ttggcctcat gctcggcctg ctctcgggca tgacgctctt
     1141 tggcccgcgc ctcgatcttc gcgcgcgcct ccgcgagctt gcgcaaccgc tcttccctcc
     1201 gctcaagttc ttccgggatc gaaaaaccat caggaatatc ggcctgatcg gccgcttcgg
     1261 ccttcgccat cagatcggcc acctcagcct tcagctgcgt ttcgatgtta ccagcgtgct
     1321 cgtaggacaa cgcactgtgc cggctcgcat tggcgtggat cttggtcccg tcaagcccaa
     1381 tcgtgcccag cttcagaacc ccgatctccc gggcaagaac cagcacctgc aaaaacaggc
     1441 ccgcgatctg cggcagaaat cgccgccgaa acgtcgcgat cgtatcgtga tcgggatgat
     1501 cgttcgccgc gatgaagcgg aacgccaccg agtcatatgt cgcacgctcc agcttgcggc
     1561 tcgaaaacac gcccgtcgca tagccataga ccagaagccc cagcaaaacc tccgggtgat
     1621 acgacgccga gcccgaccca cgataacttc cgctcatcga ccgcaggtca agacccgcga
     1681 tcacttccac aataaaacgc gccagatgcc gctccggaag ccactcatcc accgacggcg
     1741 gcagtaaaaa gccggtcgca cggtctattt ggcgaaagtt gctcatcgca aaacccagac
     1801 ggagaatcaa aatcggtctc ccgattctac ccaatatccc ggtaatgcgt taagtccgac
     1861 aggctgctag ttcgtcgcac ccgccgggat cggctccatg ccgctcttga cgatcacagc
     1921 gctcgccatc ggagacgcaa gaaatttgat cagcgccttg ccggcgtcgg gctctttcga
     1981 gacgctggcg atgccggctg aaaacactgt gaccttctgc aactccgatg gtaattgccc
     2041 gacgatgtcg atgccctgca ccggcagcag ttcgctgacc tgctggaagc cgatctcggc
     2101 atcgccgcgc gccacgattt cgccgaccgg ggtggccgga attttcctgg ccttgtcctt
     2161 catggcatcg gcaatgccga gtttctcgaa catttcggtc gagacataga cgccgctggc
     2221 agagtcggaa taggcgatcg ttttcgtggc caacagcgcg cgcttcaccg cgtcggcaga
     2281 gctgatgtcc ggcttcggcg cgcccgattt caccgcgatg ccgatcggcg atttcacgag
     2341 atcgacgcgg ctgtcggcaa cgaccttgcc ctgcttgacc agttcaccca gcgcatagcc
     2401 gaccatgatc aggacgtcgg cgggctcgcc gcgttccagc cggatcggga tcgcattgac
     2461 ggtggtcccc atcgagggcc cgtaagccgt cagcaccgtg ttgccggtca agcgctcgaa
     2521 ctccgggacc agttc
//
LOCUS       DASWGM010000292         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1826754,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000292 DASWGM010000000
VERSION     DASWGM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826754"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VGA15_08520"
     CDS             <1..1072
                     /locus_tag="VGA15_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9587774.1"
                     /translation="KGAMLSHGNLTSAVSIYDVWGKAARAKRDAVERVICVLPLFHIY
                     ALTVILLRSLERGDLISLHQRFEVEAVMRDVEVKRATAFPGVPTMWIAIASLPDLESR
                     DLSSLVSCGSGGAPLPVEVAKIFERKTNMKLKSGWGMTETCSPGTGHPSEGPEKPGSI
                     GLMLPGIEMDVVALDDPRKILPPGEVGEIRIKGPNVTKGYWNRPQETAEAFVGDRFLT
                     GDIGYMDSDGYFYLVDRKKDLIISGGFNVYPQMIEQAIYEHPAVQEVIVIGIPDDYRG
                     EAAKAFVKLRNSGVPFTLEELKVFLAGKLGKHEIPAALDFVDDLPRTSVGKLSRHELR
                     NQLPLQQPAQPQQQQLASGGRS"
     gene            1270..>2074
                     /locus_tag="VGA15_08525"
     CDS             1270..>2074
                     /locus_tag="VGA15_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9587775.1"
                     /translation="MDLSFSKEEVAFRDEVRNFFKDNVPSATRQKLQEGRHLSKQEMI
                     DWWRILNKKGWGVSHWPKEYGGTGWTSVQHYIFNEELQMAPAPSPLAFGVSMVGPVIY
                     TFGSEAQKKHYLPRIANVDDWWCQGFSEPGSGSDLASLKTKAERKGDFYVVNGQKTWT
                     TLAQHADWIFCLCRTDPSAKKQSGISFILIDMKTKGITVRPIQTIDGGFEVNEVFFDD
                     VKVPVENLVGEENKGWDYAKFLLGNERTGIARVGVSKERIRRIRELASKV"
BASE COUNT          426 a          602 c          653 g          393 t
ORIGIN      
        1 gaagggcgcg atgctcagcc atggcaatct gacttccgcg gtgtcgatct atgatgtctg
       61 gggcaaggcg gcgcgggcca aacgcgacgc cgtcgagcgc gtgatctgtg tgctgccgct
      121 gtttcacatc tacgcgctga cggtgatcct gctgcgcagt ctcgagcgcg gcgatctgat
      181 ctcgctgcac cagcgtttcg aggtcgaggc ggtgatgcgc gacgtcgagg tcaagcgggc
      241 gaccgcgttt cccggcgtgc cgacgatgtg gatcgcgatt gcaagtcttc cggatctgga
      301 aagccgcgac ctgtcgtcgc tggtcagttg cggatcgggc ggcgcgccgc tgccagtcga
      361 ggtcgcaaaa attttcgagc gcaagaccaa catgaagctc aagagcggct ggggcatgac
      421 ggagacctgt tcgcccggca ccggccatcc ctcggagggg ccggagaaac cgggctcgat
      481 cggcttgatg ctgcccggca tcgaaatgga cgtcgtcgcg ctcgatgatc cccgaaaaat
      541 cttgccgccg ggcgaggtcg gcgaaatccg catcaagggg ccgaacgtca ccaagggtta
      601 ctggaaccgg ccgcaggaaa ccgcggaagc tttcgtcggc gatcgtttcc tgaccggcga
      661 tatcggctac atggattctg acggctattt ctatctggtc gaccgcaaga aggacttgat
      721 catctccggc ggcttcaacg tctatccgca gatgatcgag caggcgatct atgaacaccc
      781 ggccgtgcag gaagtgatcg tgatcggcat tcccgacgat taccgcggcg aggcggccaa
      841 ggcatttgtc aaattgcgca acagcggggt tccgttcacg ctggaagaac ttaaagtttt
      901 cctcgccggc aagctcggca agcacgaaat tcccgcggcg ctcgacttcg tcgacgacct
      961 gccgcggacg tcggtcggca agctctcgcg ccacgaattg cgcaatcaac tgcctttgca
     1021 gcaacccgcg cagccccaac aacagcaact cgcatcagga ggtcgttcgt gaccggggca
     1081 gttcggtaac ccgtcgtccc cgcgaaggcg gggacccata cggcgaggac ttagtgtttt
     1141 ggcattgtgg tggaggcctt ttgttatcca ccaacgccag gggttatggg tccccgcgtt
     1201 cgcggggacg acgagacgaa agataacgcg taactacgaa tggtattcat tcgctcacag
     1261 gaggatccga tggatctcag cttcagcaag gaagaagtgg cgtttcgtga cgaagtgcgg
     1321 aatttcttca aggacaacgt gccatccgcg acgcggcaga aattgcagga gggccgtcat
     1381 ctctcaaagc aggagatgat cgactggtgg cgcatcctga acaagaaggg ctggggcgtg
     1441 tcgcactggc cgaaggaata tggcggcacc ggctggacct cggtgcagca ttacatcttc
     1501 aacgaggaac tgcagatggc gccggcgccg tcgccgcttg cttttggcgt cagcatggtc
     1561 ggccccgtca tctacacctt cggcagcgag gcgcagaaaa aacattatct gccgcgcatc
     1621 gccaacgtcg acgactggtg gtgccagggt ttttcggagc ccggctccgg ctccgatctc
     1681 gcctcgctga aaaccaaggc cgagcgcaag ggggatttct acgtcgtcaa cggccagaaa
     1741 acctggacca cgctggcgca gcacgccgac tggatcttct gtctctgccg caccgatccc
     1801 tcggccaaga agcagtcggg catctcgttc atcctgatcg acatgaagac caaaggcatc
     1861 acggtgcgtc cgatccagac catcgatggt ggttttgagg tcaacgaggt gttcttcgat
     1921 gacgtcaagg ttcccgttga aaatctggtc ggagaggaaa acaagggctg ggattacgcc
     1981 aaattcctgc tcggcaatga gcgcaccggc atcgcccgcg tcggcgtttc caaggagcgg
     2041 atccgccgaa tccgggagct ggcgtcaaag gtcg
//
LOCUS       DASWGM010000293         5195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_858471,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000293 DASWGM010000000
VERSION     DASWGM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858471"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VGA15_08530"
     CDS             complement(<1..579)
                     /locus_tag="VGA15_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX9587776.1"
                     /translation="MSAVERLEDRRGETPPRRDPAASEAPAVEAELTPLQGGSGARLR
                     PLLALAPYVARYRGRAILALVSLTIAAITTLIVPVAVRRMIDFGFTPEGIALINSYFS
                     VMIAVVAVLAAASASRFYLVMTIGERIVADLRGDVFAHLISLSPAFFDSARSGELISR
                     LTADTTQIKSAVGASVSIALRNLMLFIGAAVMM"
     gene            768..995
                     /gene="rpmE"
                     /locus_tag="VGA15_08535"
     CDS             768..995
                     /gene="rpmE"
                     /locus_tag="VGA15_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX9587777.1"
                     /translation="MKAEIHPEYHMITVVMTDGTEYQTRSTWGKAGDKLNLDIDSKSH
                     PAWIGGAQQLLDRGGRVSRFQKKFSGFLKKE"
     gene            complement(1090..1602)
                     /locus_tag="VGA15_08540"
     CDS             complement(1090..1602)
                     /locus_tag="VGA15_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX9587778.1"
                     /translation="MSERLQGETALVQFSERLTNSAAFGTLFRDGMDLVEETAAYLDG
                     AGRTEAKALDRAVSLTYATESMRLTTRLMQLASWLLLHRAVKEGEMTLSQANREKTKV
                     KLSAADPGPDEMLEKLPEQLQVLIARSMILQTKVRRLDTTIHAPVSERASIGNPLVPQ
                     LNRLKAAFEQ"
     gene            1668..1808
                     /locus_tag="VGA15_08545"
     CDS             1668..1808
                     /locus_tag="VGA15_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587779.1"
                     /translation="MMNKSLRAREPKSSRNRFYGFQTMRWRKEDAEKAHAKKRAAVSG
                     GF"
     gene            2171..3598
                     /locus_tag="VGA15_08550"
     CDS             2171..3598
                     /locus_tag="VGA15_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587780.1"
                     /translation="MKNRTVAIGAALVGAVLIGVLIWDWNRPSTSGLPPAASGETPKA
                     SLSLLSPGMLSIAPGSGGTGAQGLALQGDGTIVVGATFASESAPGLSKSRGVVLRMTS
                     NGQLANPAVTLITDTPTRVEGISTARDGTIVVIGSSEGPKGHFLLARLLPDGALDPAF
                     GGGGAVLANMKASMFPGDTAQAVAIQADGKIVATGVAGYAAGPLAQGGYCAIARFNRD
                     GRFDRSFGDSGRLLTSVEGKQFCGASSVLVAPDGKIIVVGHYHAEHELPHIAVFRYLP
                     DGTPDRQFGRDGIAQLLKISAIAWGGAAFDSQGRIVVVGTEHVGSTNWRFLVVRFDPN
                     GNLDQSFGTDGIASLAGGSFSQQLVAVALQQDGKIVAVGKNGWHSGGRPAEPGKRDEL
                     VVTRLDANGTLDQSFAGGGLLIMASPRYLWGARAIAIQPDGKLLIAGGFVDDANEHAH
                     SAIVLLRLNPDGTPDADFGSGLDTP"
     gene            complement(3833..4063)
                     /locus_tag="VGA15_08555"
     CDS             complement(3833..4063)
                     /locus_tag="VGA15_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX9587781.1"
                     /translation="MHRRAGTHRRWRHGPRISGAPLARCTAHEIGQDLSLLSVEELTE
                     RIALMTSEIERLQVAMTKKRASRDAANNFFKS"
     gene            4222..4536
                     /locus_tag="VGA15_08560"
     CDS             4222..4536
                     /locus_tag="VGA15_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9587782.1"
                     /translation="MAYATALAVLADPTRRKVFERLRAGPRPVNALAAGLPVSRPAVS
                     QHLKALKDAGLVEERSEGVRRIYSLRREGLTELRDWLDTFWDDALAAFKEEAEKTKGV
                     LE"
     gene            4533..5012
                     /locus_tag="VGA15_08565"
     CDS             4533..5012
                     /locus_tag="VGA15_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9587783.1"
                     /translation="MNAPTVIAPVRKSVRVKAPIAHAFDVFTNGLTRWWPLDHGVGKK
                     PIQKVLMEPRLGGRWLEIAEDGTQTSVATIILWEPPHRLVMVWQINAQWKPDLAMKSE
                     VDVRFTADGPNATLVELLHHKFETMGAESGTSMRNDVDRGWPGLIEHYAQEAERSSA"
     gene            5049..>5195
                     /locus_tag="VGA15_08570"
     CDS             5049..>5195
                     /locus_tag="VGA15_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9587784.1"
                     /translation="MSEFTVYSIPGSPFGRAVLATLEEKAATYRLMPVAPGTFRSPEH
                     LARHP"
BASE COUNT         1022 a         1570 c         1624 g          979 t
ORIGIN      
        1 catcatcacg gcggcgccga tgaacagcat caggttgcgc agcgcaatcg ataccgacgc
       61 gccgaccgcg gatttgatct gggtggtatc ggcggtgagc cgcgagatca gctcgccgct
      121 gcgcgcggaa tcgaagaatg ccggcgacag cgaaatcagg tgcgcgaaca cgtcgccccg
      181 cagatccgcg acgatgcgct cgccgatggt catgaccaga taaaaccgcg acgcgctggc
      241 ggcggccagc accgcgacca ccgcgatcat cacgctgaaa tagctgttga tcagggcgat
      301 gccttcgggg gtgaacccga agtcgatcat ccgtcgcacc gcgaccggca cgatcaaggt
      361 cgtaatcgcg gcgatggtca gcgagacaag ggcgagaatc gcgcgtccgc ggtaacgcgc
      421 cacatagggc gccagcgcca gcagcggacg cagccgcgcg ccgctcccac cctgcaacgg
      481 cgtcagttcg gcttcgacag cgggcgcctc cgaggcggcc ggatcgcgcc gcggcggcgt
      541 ttcgccgcgc cggtcttcaa gacgttccac tgcgctcatg agattttgac ccatcgccgt
      601 taccaccccc agataggccc cgccgtcacc actggcaaat cctaagaaag gtacgaaacc
      661 aagaacatac gtggcttgtt ttggcgggcc tgctgcgtta tagagcggcc caaatccgtc
      721 cccatagcca tctcgaagac aggcgccggc gcgccgaagg atctgccatg aaagccgaga
      781 ttcatccgga atatcatatg attacggtcg tgatgaccga tggcaccgag taccagacgc
      841 gctccacctg gggcaaggcc ggcgacaagt tgaacctcga tatcgattcc aagtcacacc
      901 cggcctggat cggcggcgcc cagcagctgc tcgatcgtgg cggccgtgtg tcgcggttcc
      961 agaaaaagtt ttcgggcttc ctcaagaagg agtgaggcct gccgtccctg gcctgtagca
     1021 gacatcgaaa cgcttcggct cgcgccgggg cgtttttcat ttcaggtggt ggatcgaaaa
     1081 agcctgcgct tactgctcga acgccgcctt gaggcgattg agctgcggca ccagcggatt
     1141 gccgatcgac gcgcgttccg acaccggcgc gtggatggtg gtatcgaggc ggcggacttt
     1201 cgtctgcagg atcatcgaac gggcgatcag gacctgcagc tgctccggca gcttctcgag
     1261 catctcgtcc ggacctggat cggcggccga gagcttgacc ttggtctttt cccgattggc
     1321 ctggctcagc gtcatctcgc cttcctttac cgcgcggtgc aggagaagcc acgacgccag
     1381 ctgcatcagc cgcgtggtga gccgcatgct ttcggtggcg taagtgaggc tgacggcgcg
     1441 gtcgagcgcc ttggcctcgg ttcggccagc gccgtcgaga taggccgcgg tctcttcgac
     1501 cagatccatg ccgtcccgga acagggtgcc gaatgcggcc gaattggtca gccgctcact
     1561 gaattgcacg agggcggttt cgccttgcaa acgttccgac atacttaacg ccccacgcaa
     1621 ctgttcacgt ttgtgtacat atagccggcc tcgatacgcg ccggcttatg atgaacaaat
     1681 cattgcgcgc ccgcgagccg aagtccagcc gcaaccgctt ttatggtttc cagacgatgc
     1741 gatggcggaa ggaagatgcg gaaaaagcac acgccaaaaa aagagccgcc gtttccggcg
     1801 gcttttgaag ttgataacag ggaggcgtca aacagagtgg acaggagcca ctcggtgtcc
     1861 aaccaaggac aagccaagtc ataaacgaga aagcttaatc aactgtaaac aagcggtttt
     1921 cttcagaatt cgtttgccat gtcggccatt ggcctaggct ccgaacccgt caggtgaatt
     1981 tccacatcga acgtttcgtg attgaacatc tccgaaggga gaccacggat ggatcgagat
     2041 cacttttggc tggacgatgg aataggttcg cgcggctgga tgcccgtctc gcacccgata
     2101 cccgcggcaa accaagggtc gacgaccgcc gcgcggctcg cccataattc ctcgtcgttt
     2161 ggagccgatc ttgaaaaata ggacagtggc aataggcgcg gcgctcgtcg gagcggtttt
     2221 gatcggcgtg ctgatttggg attggaatcg gccgagcacg tcgggtctac ctcctgccgc
     2281 atcaggcgaa acacctaagg ccagtttgag tctgctctcg ccgggcatgc tgagcatcgc
     2341 acccggatca ggaggtacgg gtgcgcaagg tctggcgctg cagggagacg gaacaatcgt
     2401 cgtcggcgct acgtttgcga gcgagtccgc accgggttta agtaaaagcc gtggtgtggt
     2461 cttgcgaatg acctccaacg gtcagttggc gaatccggcg gttacgctca tcaccgacac
     2521 gccaaccaga gtagagggca tttcgaccgc acgcgacgga acgatcgtgg tgatcggctc
     2581 gtccgagggg cccaaggggc atttccttct cgcgcgcctg ctgccggatg gcgctctcga
     2641 tcccgcattt ggtggcggcg gcgccgtgct ggccaatatg aaagcgagca tgttcccggg
     2701 cgacactgca caagccgttg cgatacaggc cgatggcaag atcgtcgcga caggcgtcgc
     2761 cggctatgcc gctggtcccc ttgcccaagg tgggtactgt gcgatagctc ggttcaaccg
     2821 agacggtcgt ttcgatagga gtttcggaga cagtgggcga ctgcttacgt ctgtggaagg
     2881 gaaacaattt tgtggtgcct catctgtgct cgtcgcgccg gacggaaaaa tcatcgttgt
     2941 cggacactac cacgcggaac atgaacttcc gcatatcgcc gtttttcgat acctcccgga
     3001 cggcacgccg gaccggcagt tcgggcgaga cggcatcgcc caactgttaa aaatttcggc
     3061 gattgcatgg ggcggcgcgg ccttcgactc gcagggcaga attgtggttg tcggaacaga
     3121 acacgtaggg tcaaccaact ggcgattcct ggtcgttcgc ttcgacccca atgggaacct
     3181 cgatcagtcc ttcggcacag atggcatcgc ctccttggcc ggaggtagtt tttctcagca
     3241 gcttgtagcc gtcgcgctgc agcaggacgg caagatcgta gcggtgggca agaacgggtg
     3301 gcatagcggg gggcgtcctg ccgagcccgg aaagcgcgac gagcttgttg tcacgaggct
     3361 agacgcgaac ggtacgttgg atcaatcgtt cgccggtggc ggtttgctta tcatggcttc
     3421 gcctcgatat ctctggggcg cgcgcgcaat cgcgattcag ccggatggga agctgctgat
     3481 tgccggcggg tttgtcgatg atgcaaatga gcacgctcat tcggcaatcg tgctcctcag
     3541 actgaaccca gacggcacgc cggatgcaga ctttggaagc ggcttggaca cgccctgaac
     3601 gactggcgag gccccgttag gctccaatat ctcgccgcga aggctaatcg cgggctagtt
     3661 tgagacaatt tctccgcagt cgtccccgcg acgcggggat ccataagccc ggcggttttg
     3721 aacggcaggc gtccactaca ctgcccgaac gagagggtac ggcgtatggg tcccggcgtt
     3781 cgcctggcgt tcgccgggac gacaactagt gtgccctgct cacgtttcgc attcacgact
     3841 tgaagaaatt gttagcggcg tccctggagg cgcgcttctt ggtcatggcc acctgcagcc
     3901 gctcgatttc cgacgtcatc agcgcgatgc gctcggtcag ttcctcgacc gacagcagcg
     3961 acaggtcctg cccgatctcg tgggctgtgc agcgcgcaag cggtgcgccg ctgatccggg
     4021 gcccatgacg ccacctgcga tgggtcccgg ctctgcgatg catcgctgaa gaagcgctgc
     4081 atcgcgtccg ggacacgtac cgcgcgcgtc ggcggcattt cttgcgactt agatgacagt
     4141 tgcggcctga catcctgggc gacttttggt tgcgcaaaaa tcgttcttga ctatcgcgta
     4201 agccatgact taccgttagt catggcttac gcaactgccc tcgccgtcct cgccgacccg
     4261 acacgccgaa aagtgttcga gcgcctgcgt gcgggaccgc gcccggtaaa tgccctcgcc
     4321 gccggcctgc cggtctcgcg cccggcggtc tcgcaacacc tcaaggcgct gaaagacgca
     4381 ggtctcgtcg aggagcgcag cgaaggggtg cgccggatct attcgctgcg gcgcgagggc
     4441 ctgacggaat tgcgtgactg gctcgacacc ttctgggacg atgcgttggc ggcgttcaag
     4501 gaggaagcgg aaaaaaccaa aggagttttg gaatgaacgc cccgaccgtc attgcccccg
     4561 tgcgtaaatc cgtgcgtgtg aaagcgccga tcgcgcacgc tttcgacgtc ttcaccaacg
     4621 ggcttacccg ctggtggccg ctcgaccatg gcgtcggcaa aaaacccatc cagaaagttc
     4681 tgatggagcc acgcctcggc gggcgctggc tcgagatcgc ggaagacggc acgcagacct
     4741 ccgttgccac gatcatcctg tgggagccgc cacatcgcct ggtgatggtc tggcagatca
     4801 acgcccaatg gaagccggac ctggcgatga aatcggaagt cgacgtgcgc ttcacggccg
     4861 atgggccgaa cgcgacgctg gtcgaactgt tgcaccacaa attcgaaacc atgggcgcgg
     4921 agtccggaac ttcgatgcgc aatgatgtcg atcgcggctg gcccgggctg atcgagcatt
     4981 atgcgcagga agctgaacgc agcagcgcgt gaaacaagca acgctcaatc ttcaacaagg
     5041 aggcagccat gtccgaattc accgtctatt ccattcccgg cagcccgttc ggtcgcgcgg
     5101 tgctggctac gctggaggag aaagccgcga cctatcgcct tatgccggtg gcgcccggca
     5161 cgttccgctc gccggagcat ctcgcccgcc atccc
//
LOCUS       DASWGM010000294         2409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1966059,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000294 DASWGM010000000
VERSION     DASWGM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1966059"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VGA15_08575"
     CDS             complement(<1..867)
                     /locus_tag="VGA15_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HEX9587785.1"
                     /translation="MADDDASDKTEDPTQKRLEDAHERGDVAKSQEINTWFIIAGATL
                     VLSTFSASIGGGILMPLRNLIANSWMIHADGPGLLALAQSLEYALIAALGVPLLMLSL
                     AAIGGNMIQHRLVWSGEQLKPKFSKVSPGAGFKRVFGKQAAANFAKGLFKLVALGAVM
                     LAVLWPERHRLESMVRFDPAAILGATTSLTLQLMGAVVAMLAVVAIADYFFQYRQWYQ
                     RQKMSLQEMKEEFKQSEGDPHVKGRIRQLRHARMKKRMMAAVPKASVIITNPTHYAVA
                     LSYERGMPAPICV"
     gene            complement(880..1650)
                     /gene="fliR"
                     /locus_tag="VGA15_08580"
     CDS             complement(880..1650)
                     /gene="fliR"
                     /locus_tag="VGA15_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX9587786.1"
                     /translation="MRIDISLLPALAAAFMLAFARIGAMVMLLPGLGESNIPVRVKLG
                     IALLLTLVILPLHRAAYHIDMQSLTPLLVLMVHEIVIGVVLGATARVTLSALQVAGSV
                     IAQQLGLGFVTAVDPTQGQQGLLIGNFLTILGLTLLFATDSHHLVIAALNDSYSIFSP
                     GELMPSGDVAALATRAFAAAFRIGMQLSAPFLVFGLVFNIGLGVLARLMPQMQVYFVG
                     VPLSILAGFLIFALVIAAMMGTFLDYFVGVMHDLIPLS"
     gene            complement(1668..1931)
                     /gene="fliQ"
                     /locus_tag="VGA15_08585"
     CDS             complement(1668..1931)
                     /gene="fliQ"
                     /locus_tag="VGA15_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX9587787.1"
                     /translation="MTGAETLDVARDAIWTIVVVSSPLMVVGLVVGVVVSLFQALTQI
                     QEQTLVFVPKILAIFVTLLLALPFMADSLHSHMMRISSRIIGG"
     gene            complement(2043..2354)
                     /gene="fliE"
                     /locus_tag="VGA15_08590"
     CDS             complement(2043..2354)
                     /gene="fliE"
                     /locus_tag="VGA15_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX9587788.1"
                     /translation="MASPTVAANAYASLARILDTGGAGKASEGGGGPSFGALLKDAIG
                     SVLDAGKKSDAQTVAMASGKANVMDVVTAVAETDVAVSTLVSVRDRVIQSYEDIMKMP
                     I"
BASE COUNT          491 a          752 c          728 g          438 t
ORIGIN      
        1 gacgcagatc ggcgccggca tgccgcgttc gtagctcagc gccaccgcgt agtgggtcgg
       61 gttggtgata atcaccgagg ccttgggaac cgcggccatc atgcgcttct tcatgcgcgc
      121 gtgccgcaac tgcctgatcc gacccttgac gtgcgggtcg ccttcggatt gcttgaattc
      181 ttccttcatt tcctgcagcg acatcttctg ccgctggtac cattgccggt actggaagaa
      241 gtagtccgcg atcgcgacaa ccgcgagcat cgccacgacg gcgcccatca gttgcagcgt
      301 caggctggtg gtcgcgccca aaatcgccgc cgggtcgaac cgcaccatcg attccagacg
      361 gtggcgctcc ggccaaagca ccgccagcat caccgcgccc agcgccacca gcttgaacag
      421 gcccttggcg aagttcgccg ccgcctgctt gccgaacacc cgcttgaacc cggctccggg
      481 cgagaccttg ctgaatttcg gcttgagctg ttcgcccgac cataccagcc ggtgctgtat
      541 catgttgcct ccgatcgccg cgagcgacag catcaaaagc ggcacgccga gggccgcgat
      601 cagcgcgtat tcgaggctct gcgccagcgc caacaggccg ggcccgtcgg cgtggatcat
      661 ccaggaattg gcgatcaggt tgcgcagcgg catcaggatc cccccgccga tcgatgcgga
      721 aaatgtcgac agaacaaggg tcgcgccggc aattatgaac caggtgttga tttcctggct
      781 tttggcgaca tcgccgcgct cgtgggcgtc ttccagacgt ttttgcgtcg ggtcttctgt
      841 tttatcggat gcgtcgtcgt cggccatggt tccaggcttt cagctgagcg ggatcaggtc
      901 gtgcatgacg ccaacaaaat aatcgagaaa ggtccccatc atcgccgcga taacgagggc
      961 gaaaatgagg aatccggcaa gaatcgatag cggcacgccg acgaaataaa cttgcatttg
     1021 cggcatcagc cgcgccagca cgccaagccc gatattgaaa accaggccaa aaaccagaaa
     1081 cggcgccgaa agctgcatgc cgatccgaaa cgcggcggcg aaggcgcggg tggccagtgc
     1141 cgcgacgtcg ccgctcggca tcaattctcc cggcgaaaag atcgaatagc tgtcgttgag
     1201 ggcggcgatg accagatgat ggctgtcggt cgcaaacaat agcgtcaggc cgaggatggt
     1261 gaggaaattt ccgatcaaaa ggccctgctg accctgggtc ggatcaacag cggtgacaaa
     1321 tccgaggccg agttgctggg cgatcaccga tcctgcgacc tgcagcgccg aaagcgtgac
     1381 ccgcgcggtg gcgccgagca cgacgccgat gacgatctcg tgaaccatca agaccagcag
     1441 cggcgtcagc gactgcatgt cgatgtgata ggcggcgcgg tgcagcggca gaatcaccag
     1501 cgtcagcaac agcgcaatcc caagcttgac ccgcaccgga atattgctct cgccgagccc
     1561 cggcaacagc atgaccatcg cgccgatgcg ggcgaatgcc agcatgaagg cggcggcgag
     1621 cgcgggcagc agtgaaatat cgatgcgcat gattcgtgca tactgcatca accgccaatg
     1681 attcgcgacg atatccgcat catatggctg tgcagcgagt ccgccataaa aggcaacgcc
     1741 agtagcaggg taacgaagat tgccaggatt ttcggcacga acaccagcgt ctgttcctgg
     1801 atctgggtca gcgcctggaa cagcgacacc acgacgccga ccacaagacc gacaaccatg
     1861 agtggcgatg acaccacgac gatggtccag atcgcatcgc gcgccacgtc gagggtttcg
     1921 gcaccggtca tttttctatc tcgttctatt tgatttgcgt tcgtcattcc ggggcagtcc
     1981 gcaggactga acccggaatc tcgagattcc gggctcgcgc ttcgcgcgcc ccggaatgac
     2041 gatcagatcg gcatcttcat gatgtcttcg taggactgga tgacgcggtc gcgcaccgac
     2101 accagggtcg agaccgcgac gtcggtttcc gccaccgccg tcaccacgtc catcacgttg
     2161 gccttgcccg acgccatcgc caccgtctgc gcgtcggatt tttttccggc gtccaggacg
     2221 cttccgatcg cgtccttgag cagcgccccg aaggatggac cgccgccccc ttcggaagcc
     2281 ttgccggcgc cgccggtgtc cagaatgcgg gcgaggctgg cataggcatt ggcagcgact
     2341 gtgggtgaag ccatggttta aggttccctg ttcaggcctt gaggatgtcg agcgtgcgtt
     2401 gaatcatcc
//
LOCUS       DASWGM010000295         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1133736,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000295 DASWGM010000000
VERSION     DASWGM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133736"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VGA15_08595"
     CDS             <1..195
                     /locus_tag="VGA15_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587789.1"
                     /translation="FDADLDHKNYYIAHETIVRRAVGSAIEPIPFAIFSFLNRIASRA
                     PDFFKIPHDAIIEVGFRVEV"
     gene            complement(241..792)
                     /locus_tag="VGA15_08600"
     CDS             complement(241..792)
                     /locus_tag="VGA15_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587790.1"
                     /translation="MRVAVFVVFVSILATASEASPSCMTRTEAHRHFGSAHIYWHGAG
                     HCWDATSTRQHHQVHHVREKSTHHVREKTEHQAKPKIDHPRWHESMSQMLAADEEPVR
                     TSWLDRWVDIEPAQSPLGARWVDIVQAAPAQMPAQIMESESEPIITPRSVMMVITVLT
                     LTLAIVEFLIIGMRLAGPPRRWL"
     gene            2047..>2991
                     /locus_tag="VGA15_08605"
     CDS             2047..>2991
                     /locus_tag="VGA15_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9587791.1"
                     /translation="MAATRIDCDIHPAVGGTRTTLLPYLDDHWKEQVVSRAIDGLDLT
                     SYPPNMPLSGRADWRPANGKPGSDLAMVQRGAFGQLGASHAICNVLYGAQAVFDPYMA
                     AAFCKAINDWIAAEWLARDPRLRASIVVPMQAPDLAIEEIERRAGDNRFVSVLVLAQG
                     ETLLGRRHYWPVYQVAEKHKLPIAVHAGSQYRMAPSSIGWPSYRYEYYLAEAQAFQAQ
                     VLSLIYEGVFLKFPGLKVVLMESGVSWLPAFMWRANKTWRGVRVEVPWIDREPAAIMR
                     DHIRLTTQPFDAPPDAAGVADIIDMIGSDKMFLFASDYP"
BASE COUNT          548 a          907 c          925 g          611 t
ORIGIN      
        1 ttcgacgccg atctcgacca caagaactac tacatcgccc acgagaccat cgttcgccgc
       61 gccgtcggat cggcgatcga gccgattcct tttgcgattt tctcgtttct gaatcgtatc
      121 gcgagccgcg ccccggattt cttcaaaatt ccccacgacg ccatcatcga ggtgggattt
      181 cgggtcgagg tctgattgca ggcggcctcg cacaagctca tgcgggccaa cggctccgga
      241 tcaaagccag cgacgcgggg gtccggccag tctcatgccg atgatcagaa actcgacgat
      301 cgccagcgtc agcgtcagga cggtgatcac catcatcacg ctgcgcggcg tgatgatcgg
      361 ctcggattcg ctttccatga tctgcgccgg catctgcgcc ggcgcggcct ggacgatatc
      421 gacccagcgc gcgccaaggg gggactgcgc cggctcgatg tccacccagc ggtcgagcca
      481 cgatgtccgc accggttctt catcggcagc caacatctgc gacatcgatt catgccatct
      541 gggatgatcg atttttggct tggcctgatg ctcggttttt tcccgaacgt gatgagtgga
      601 tttttcccga acgtgatgga cctggtgatg ctggcgtgtc gatgtcgcgt cccagcaatg
      661 gccggcgcca tgccaataga tgtgagctga accgaaatgc cggtgcgctt cggtcctggt
      721 catgcaggac ggtgaagctt ccgatgccgt agccagtatc gacacaaata caacaaatac
      781 cgcgacacgc atcggtcgct cctgaaatac gctttactca acgaaatcga atcacttggt
      841 aagctttggc tgacttcaag gcgaaatttg gaaaccgatt gccggttatg catggtttaa
      901 gggaccgaat gtccgcctgt taaggtcatc gactgccaca ttcacgatct atcgtggcgg
      961 caccatcgcc gtcactgtct cgatcgcacc gactcgaatt gttacgtact acatgctgga
     1021 ttgtagagcg gccgcgccaa tcgttccccg gatgctgcgc tgcgccacca gcgcgtttac
     1081 gcgcgtcttt gacgcgccat tagcgcgttt acgcgcgtcg ttgacgcgcc attagcgcgt
     1141 ttacgcgcgt ctttgacgcg ctatgacttg cggcgtggtg cgctgctgat ccggggccca
     1201 tcgctggctc tgctatgggt cccggctctg cggtgcatcg taaggacgct gcgccgcgtc
     1261 cgggacacga gcggaatttg ctctcgctgc cgtgcggtga tttgcccgtc gggcggtttg
     1321 ttgacagggg tgtccagccc tctttgcaaa aatatttctg tttccgtacc ccccaaatca
     1381 catctagaac ttctcgcatc ccaccccaca agaggggcgt atcatgatcg tcacggacgt
     1441 aggggcggga tgcggtggac gcggcagcgt tttgcgcgcg atgggatcgc agggcaggtc
     1501 gaaagacttg tgagcgatta ccggcacgct gacgagggat gctgcagcgg acggcaaaat
     1561 cgtgtggtcc tgacgccccg acgctggcgt caagttttgc ggaaggcaag tcggccctac
     1621 cgggtgcgga caagccagat atccgtgaga tgacggtggc aagagagccc ggtcaccggg
     1681 gagagcacga aataagccgt taaaaccatt gcgtgcggga atgccgggtg tccggcgtac
     1741 tcgtggtgac taacgcgtgt gctttctaca ctgcacacgc ggctgcgggt gcgcggcacc
     1801 cggcattccc cacgccctct gttttcttgg gcggaagata aacgcacggc tcgggcgcat
     1861 cgcgtcgcgg ggtcgcgaac gcatgtctgg atttgtcgtc actgcgagga gcgaagcgac
     1921 gaagcaaaat cctcttgcat ggctgccccg ttttggccgc tcaacccttg cgcttctggg
     1981 ccatcggcgg cacatttgaa gcgcggcatg gttcgccgcc aaaaacgaca tgttagggaa
     2041 atgcgaatgg cagcgacgcg gatcgactgc gatatccatc ccgcggtggg cggcacccgc
     2101 accacgctgt tgccttatct cgacgatcac tggaaggaac aagtggtgag ccgggcgatc
     2161 gatggcctcg atctcaccag ctatccgccg aacatgccgc tgtccggccg ggccgactgg
     2221 cggccggcca acggaaaacc cggcagcgat cttgcgatgg tgcagcgcgg ggcgttcggc
     2281 cagctcggcg ccagccacgc gatctgcaat gtgctctatg gcgcgcaggc ggtgttcgat
     2341 ccctatatgg cggccgcgtt ctgcaaggcg atcaacgact ggatcgcagc cgaatggctc
     2401 gcgcgcgatc cacggctgcg cgcttccatc gtggtgccga tgcaggcgcc cgatctcgcg
     2461 atcgaggaaa tcgagcgccg cgccggcgac aacaggtttg tatccgtgct ggtgctggcg
     2521 caaggcgaga cccttttggg gcggcggcat tactggccgg tctatcaggt agccgagaaa
     2581 cacaaactgc cgatcgctgt tcacgccggc agtcagtacc ggatggcgcc gagctcgatc
     2641 ggctggccgt cctatcgcta cgaatattat ctcgccgagg cgcaggcgtt tcaggcccag
     2701 gtgctcagtc tcatctacga aggcgtgttc ctcaaattcc ccggcctcaa ggtggtcttg
     2761 atggaatccg gcgtgagctg gctgccggcc ttcatgtggc gtgccaacaa gacctggcgc
     2821 ggcgtgcgcg tcgaggtgcc ctggatcgat cgcgagccgg cggcgatcat gcgcgaccac
     2881 atccgcctga ccacgcagcc gttcgacgcg ccgcccgatg cggccggcgt cgccgacatc
     2941 atcgacatga tcggctcgga caagatgttc ttgttcgcgt cggattatcc g
//
LOCUS       DASWGM010000296         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_29218,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000296 DASWGM010000000
VERSION     DASWGM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29218"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGA15_08610"
     CDS             complement(<1..215)
                     /locus_tag="VGA15_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587792.1"
                     /translation="MAAAVETANVKPSYLDIDRNVQRAMAPGGLKYWLWIAFCAFLTA
                     LGISFWTNQIYEGLGVTGYGQPTMWAV"
     gene            complement(215..2827)
                     /locus_tag="VGA15_08615"
     CDS             complement(215..2827)
                     /locus_tag="VGA15_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9587793.1"
                     /translation="MAEVSWDDATKLFNERLAQAAKNGKDRVAFIGASQGPTLDKISR
                     QWLQAWNSSRIIFWERINDEPARAAAEACFGRRDLPVYRLDQAETIISFDADFVETWG
                     SPVEYARQYAEFRSPRMRKNGLTIGRSAYVGARLNATAAKCDEWVAANPGTEGVLAMG
                     VLNVVVTQRWVAPNAGIDAAALKNFVAPYDPDTVSKQTGVSAETIKKLGSWFGQADGA
                     VAIAGTEDQLAHIAAYILNAVTGNVGKTMVFLDGSPPEALSRADDVTALIDAMRNGQI
                     DVLVAAGGNPVYSMPPSWGFRDVAGRVGFVAWLGEVPDESAEMAHLMMPVHHALESWR
                     DTAPRAGIYGLGQPVMQPVFRSRALQDIMIASAHLAAGASAQSMPWENAADAVNAAWQ
                     DLQTKVAPSVPAADFWNQARREGGFFQAAKEVAVTLNSAVFQQTPKLTAEPTSELTLV
                     AFPHLFLYDGRGANKPWLQEMPEPVSQIVWDSWAEMHPDTAAKFGLKQDYESVQLYAG
                     INVIEITTAAGPIELAVTITPTVKPGVIAAPIGQGHTSYGRYAKGRGVNLWAFLPERM
                     RNIPVTVRLTEKRNKLITPMGKSDMMGRSIIEAMSVDDLVKGVAPEVEGEPHLDVPYE
                     MYDPHPYTGHKWGMTIDVNSCTGCSACVAACYAENNIPVVGKDQVDGGRIMSWLRIER
                     FIPNAEEAAKAPPLYIAPILCQQCDHAPCEPVCPVFASHHTPEGLNAQIYNRCIGTRF
                     CENNCPYKVRRFNWYEPEWPEPMNLQLNPEVTVRGAGVMEKCTFCIQRITVAEINARV
                     DERSLVDGEIIPACAQACPSRAISFGDINDPSSVMMKRRVDNKIRIYTMLPEFNALPA
                     ITYLRALYRQRGNA"
BASE COUNT          496 a          917 c          847 g          570 t
ORIGIN      
        1 tacaccgccc acatcgtcgg ctgcccatag ccggtaacgc ccaggccctc atagatctgg
       61 ttggtccaga agcttattcc gagcgccgtc aggaaggcgc aaaacgctat ccacagccag
      121 tatttcaacc cgcccggcgc catcgcgcgc tgaacgttgc ggtcgatgtc gaggtaactg
      181 ggcttgacgt tggcggtttc gacggcggca gccattatgc gttccctctc tggcggtaca
      241 gcgcacgcag gtaggtgatc gccggcagcg cgttaaattc cggcagcatc gtgtaaattc
      301 gaatcttgtt gtccactcgg cgcttcatca tcacgctgct cggatcgttg atgtcgccga
      361 acgagatcgc gcgcgacgga cacgcctgcg cgcacgccgg aatgatttcg ccgtccacga
      421 ggctgcgttc gtcgacccgt gcgttgatct ccgccaccgt gatgcgctgg atgcagaacg
      481 tgcacttttc catcacaccg gcaccgcgca cggtgacttc cggattcagt tgcaggttca
      541 tcggctcggg ccattcgggc tcataccagt tgaatctcct gaccttatac gggcaattgt
      601 tctcgcagaa acgcgtgccg atgcagcggt tatagatctg cgcgttgagc ccctcggggg
      661 tgtgatgcga cgcgaatacc gggcagaccg gctcgcacgg agcatggtca cattgctggc
      721 acaggatcgg cgcgatgtac aaaggcgggg ctttggcggc ctcctcagcg ttcgggatga
      781 accgttcgat ccgcagccac gacatgatgc ggcccccgtc cacctggtcc ttgcccacga
      841 ccgggatatt gttctcggcg tagcaagcgg cgacgcatgc gctgcatccc gtgcacgaat
      901 tcacgtcgat cgtcatgccc cacttgtgtc ccgtatacgg atgcgggtcg tacatctcgt
      961 acgggacgtc gagatggggc tcgccttcga cttcgggggc cactcccttg accagatcgt
     1021 cgacgctcat cgcttcgata atcgagcggc ccatcatatc gctcttgccc atcggcgtga
     1081 tgagcttgtt gcgcttctcg gtcagacgta cggtcaccgg aatattgcgc atccgttcgg
     1141 gcaggaaggc ccacaggtta acgccgcgcc ccttcgcata gcggccgtat gacgtatgcc
     1201 cttgtccgat cggcgctgct atcacgccgg gcttgaccgt aggcgtgatg gtcaccgcga
     1261 gttcgatagg tcccgccgcc gtcgtgattt cgataacgtt gataccggca tagagctgca
     1321 ccgactcgta gtcttgtttg agcccgaatt tggcggctgt atccggatgc atctcggccc
     1381 agctatccca gacgatctgg ctgaccggct ccggcatctc ctgtagccaa ggcttgttgg
     1441 cgcctcgccc gtcgtaaagg aagagatgcg gaaacgccac gagcgtcaat tcggaagttg
     1501 gttcggccgt aagcttgggc gtctgctgaa agacggcgga gttgagggtg actgcgacct
     1561 cttttgcggc ctggaagaac ccaccctccc gccgcgcctg attccagaag tcggcagcgg
     1621 gcacgctcgg agcaaccttc gtctgaaggt cctgccacgc ggcattgacc gcatcggcgg
     1681 cattctccca aggcatcgac tgcgcggacg ctcccgctgc aagatgcgcc gaagcgatca
     1741 tgatgtcctg cagcgcacgg ctccggaaga ccggttgcat caccggctgg cctagtccgt
     1801 agatacctgc gcggggggca gtatcgcgcc agctttcgag cgcatgatgc accggcatca
     1861 tgagatgcgc catctccgcg ctctcgtccg gcacttcgcc gagccacgcc acgaagccga
     1921 cgcggccggc gacgtcgcga aagccccacg acggcggcat cgagtaaacc ggattgccgc
     1981 cggccgcgac caggacgtcg atctggccgt tgcgcatggc gtcgatcagc gccgtcacgt
     2041 cgtccgcgcg gctgagcgcc tccggcggcg aaccgtcgag aaaaaccatc gtcttcccga
     2101 cgtttccggt gaccgcgttt aagatgtatg ccgcgatatg cgcgagttgg tcctcagtgc
     2161 cggcaatcgc cacggcccca tcggcttgtc cgaaccacga gccaagtttc ttgatcgtct
     2221 cagcggagac cccggtctgt ttgctgactg tatccgggtc atacggcgcg acaaaattct
     2281 tgagcgccgc agcgtcaatg cccgcgttgg gcgcaaccca tctctgcgtc acgaccacgt
     2341 tgaggacgcc catcgcgagc acgccctcgg tgcccggatt cgcggcgacc cattcgtcgc
     2401 atttggcggc ggtcgcgttg agccgcgcgc ctacgtaggc cgaacggcca attgtcagcc
     2461 cattcttgcg cattcggggc gagcgaaatt cggcgtattg gcgcgcgtat tcgaccggcg
     2521 agccccaggt ttccacgaaa tccgcgtcga acgagataat cgtctcggcc tgatcgagcc
     2581 ggtacacggg tagatcgcgc cgcccgaagc acgcctcggc cgccgcacgc gccggctcgt
     2641 cgttgattcg ttcccagaat atgatgcggc tcgaattcca tgcttgcagc cattgacgcg
     2701 aaatcttatc cagcgtcgga ccctgcgacg cgccgataaa tgcgacgcgg tccttgccgt
     2761 tctttgcggc ctgtgcgagc cgctcgttga acagcttggt cgcgtcgtcc catgaaacct
     2821 ccgccaactc
//
LOCUS       DASWGM010000297         5543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1266570,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000297 DASWGM010000000
VERSION     DASWGM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266570"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VGA15_08620"
     CDS             complement(<1..251)
                     /locus_tag="VGA15_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX9587794.1"
                     /translation="MTSERYNARESEPRWQRQWDERAIFASKNDDPRPKYYVLEMFPY
                     PSGRIHIGHVRNYTLGDVLARFMRAKGFNVLHPMGWDAFG"
     gene            616..1602
                     /locus_tag="VGA15_08625"
     CDS             616..1602
                     /locus_tag="VGA15_08625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9587795.1"
                     /translation="MSQQRVPPTPNNFHTWFKYSLGTSPDLKRAIDILVGNKRKFDVT
                     TNRDLFAAYVGPHTADDTDLNNVSQQLHSVVASAKHYLKVAIADNRSQIRAISDVADQ
                     SEVKVDPKSLIENLLNELAQAVTRATKLEARFVESTRELDTIRDSLNKSEERARTDTL
                     TGLPNRRALDEFFRKAQIAAMEQGEPLSVLLIDIDHFKTFNDNFGHGVGDQVLRLMAN
                     VLRERLRETDLPARYGGEELIAVLPGADLATCVAVAERIRRLISESRITRRSTGEILP
                     SITVSIGVGQFQFGESMADLIDRCDRALYLAKRTGRDRVVTENELDRQLEAS"
     gene            1914..2900
                     /locus_tag="VGA15_08630"
     CDS             1914..2900
                     /locus_tag="VGA15_08630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9587796.1"
                     /translation="MSQHRVPPTPNNFHTWFKYSLGTSSGLKRAIDILVGNKRKFDAT
                     TNHDLFAAYVAPNTADDTDLNNVSQQLHSVMASAKQFLTVAIADNRSQIRAISDVADQ
                     SEVKVDPKSLIENLMHELAKAATRATKLEASFVESTRELDTIRDSLNKSEERARTDTL
                     TGLPNRRALDEFFRKAQIAAMERGEPLSVLLIDIDHFKTFNDNFGHGVGDQVLRLMSN
                     VLRERLRDIDLPARYGGEELIAVLPGADLPGCEAVAERIRRAISECRITRRSTGEILP
                     SITVSIGVGQFQFGESMADLIERCDRALYLAKRTGRDRVVSENELDRQLEAS"
     gene            complement(2897..4588)
                     /locus_tag="VGA15_08635"
     CDS             complement(2897..4588)
                     /locus_tag="VGA15_08635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9587797.1"
                     /translation="MSRVTLNRKRVKLKKLLGIRARLALLALILVAPLMLERVRSLEN
                     ARSKQIALASQEFSSLAQHSADAQREMISSVETVLKSAAYIRASAGGVDRSCDILRAS
                     LPVNLPWIRTLLVVGGDGRVQCSTNDIFVGVDLSDRAYLKRARESHEIVFSDFLFAKL
                     TNKPVVMAAYPVFAINNDVDSIILAGVNLDWMSKIMSNLGGRPGVSAVLIDSTGTVLA
                     APADQASMIGRSLDAVPLLSAIAAKAIDSDQAEGTLSFVAVDGSRRAVSFARIAGTGS
                     RLIVSIDETKVSAAIDRDIRTAYLQLGFVSLFVLLGALIAAEKLIIQPIEMMAAMAKR
                     FGQGNWSVRAARGRLPSEFIPLARALNAMAARLAERERELVATNDRLTVIASIDMLSG
                     LANRRGFQSRLDFEWMKSQQYDTALSLLMIDVDHFKLFNDTYGHPEGDVCLSRLGETL
                     AGIAAETMGFAGRYGGEEFCLLLPNTDAVRALEIGETVRAAVQALAMPHATSLYQAVS
                     VGVACTKPNDAQRPGDLIEAADAALYAAKHGGRNAVVEHGFVRITDGAGTVALAS"
     gene            4696..5385
                     /locus_tag="VGA15_08640"
     CDS             4696..5385
                     /locus_tag="VGA15_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX9587798.1"
                     /translation="MTSETPSPITPHLPNGLLAVEQDIARACQQARRDRSSVTLVAVS
                     KTFDAGAISPVIEAGQRVFGENRVQEAKTKWPGLISAYPDLALHLIGPLQSNKAREAV
                     TLFDAIHSVDRPSICEALAKEIFSQKRAPQLFVQLNTGEEPQKAGVAPAEADAFIASC
                     REKYGLAISGLMCIPPVDDAPAPHFALTAKIAARNGLKNLSMGMSADFAIAIQFGATH
                     VRVGSAIFGKR"
BASE COUNT         1165 a         1674 c         1672 g         1032 t
ORIGIN      
        1 ccgaacgcat cccagcccat cggatgcagc acgttgaaac ccttggcccg catgaagcgc
       61 gccagcacgt cgcccagcgt gtagttgcgg acatgcccga tatggatgcg cccggacgga
      121 taggggaaca tctcgagcac gtaatatttc ggccgcgggt cgtcgttctt ggaggcgaag
      181 atcgcccgtt cgtcccactg gcgttgccag cgcggctcgg attcgcgggc gttgtagcgt
      241 tcggaggtca tggaatcgta agggcttttt tgtgggattc gcggccgtct tttaggacgg
      301 cggactaggc cataaaagac cgcgatgggt caatgggttg taggcaaggg aaagaccgcc
      361 gtttgcgcga cctcggagcg gaaaattcgg aaaaactaaa ctcaattctc acgcagatag
      421 ctgcagcacc cgggtattaa gccggtccga acatttgcct cgttcggatt gagccacgat
      481 taacggttcc atgggacggt gcggccgaag aatttccgca cggacacggg atcatcttaa
      541 atcgtgacag gcgacgcgtt tgacgagctt gatttcgagt acgcgacgga agtcgcggaa
      601 aaagccgtgc gatcgatgtc gcaacagcgc gtaccaccga cgccgaacaa ttttcacacc
      661 tggttcaaat attccctcgg tacctcaccc gatctcaaac gggctatcga tattctggtc
      721 ggcaacaaac gaaagtttga cgtcaccacc aaccgcgacc tgttcgcggc ctatgtcgga
      781 ccgcacaccg ccgacgacac ggacctcaac aatgtttccc agcagctcca ttcggtcgtg
      841 gcctccgcga agcactatct caaggtggcc atcgccgata accgttcgca aatacgcgcc
      901 attagcgatg tcgccgacca gagcgaagtc aaggtcgatc caaaatcgct tatcgagaat
      961 ctgctgaacg agctggccca agccgtcacc cgtgcgacga aactcgaggc gcgcttcgtc
     1021 gagtcgacgc gagagctgga cacgatccgc gactccctga acaaatcgga agagcgcgcc
     1081 aggaccgaca cgctcaccgg cctgccaaac cgccgtgccc tcgatgagtt ttttcgcaag
     1141 gcccagatcg cagcgatgga acaaggcgag ccgctcagcg tcctgctgat cgacatcgat
     1201 catttcaaga cgttcaacga caattttggg cacggcgtcg gcgaccaggt gcttcggttg
     1261 atggcgaacg tgttgcggga gcggcttcgg gaaaccgatc tgccggctcg atatggcggc
     1321 gaagaactca tcgccgtgct gccgggtgcc gacctcgcca cctgcgtggc ggttgccgag
     1381 cgcatccgcc gcttgatctc ggaaagtcgc atcacccgcc gctcgaccgg ggaaattctt
     1441 cccagcatca cggtttccat cggggtcgga cagtttcagt tcggcgagtc gatggcggat
     1501 ttgatcgatc gatgcgatcg cgcgctgtat ctcgccaaga gaaccggacg cgaccgggtc
     1561 gtgaccgaga acgaactcga tcgccagctg gaggcgagct agctcgccag cgccactatg
     1621 ccggcgccat ccgtcatccg ttatccgtac aaagccacct cgccgacctc caacgcgcgg
     1681 ctcgcgttgg tattcggcgg cagcagacaa gttccttacc gccataacgc acggcaaagg
     1741 ccgtagaatc actgaactct caatgaggtg cggttaacgc atacgtggga cgctggggtc
     1801 gacaacagga attagctcac agacgggaga tcgcttaaat cgtgacaggg cacgcgcttg
     1861 acgagcttga tttcgagtac gcgacggaag tcgcggaaaa agccgtgcga tcgatgtcgc
     1921 aacatcgcgt accgccgacg ccgaacaatt ttcacacctg gttcaaatat tccctcggta
     1981 cctcatccgg tctcaaacgg gctatcgata ttctggtcgg caacaagcga aagtttgacg
     2041 ccaccaccaa tcatgacctg ttcgcggcct atgtcgcacc gaacaccgcc gacgacaccg
     2101 acctcaacaa cgtttcccag cagctccatt cggtcatggc ctccgcgaag cagtttctga
     2161 cagtggccat cgccgataac cgttcgcaaa tacgcgccat tagcgatgtc gccgaccaga
     2221 gcgaagtcaa ggtcgatcca aaatcgctta tcgagaattt gatgcacgag ctggccaaag
     2281 ccgctacccg tgcgacgaaa ctcgaggcga gctttgtcga gtcgacgcga gagctggaca
     2341 cgatccgcga ctccttgaac aaatcggaag agcgcgccag gaccgacacg ctcaccggcc
     2401 tgccaaaccg ccgtgccctc gatgagtttt ttcgcaaggc ccagatcgca gcgatggaac
     2461 gaggcgagcc gctcagcgtc ctgctgatcg acatcgatca tttcaagaca ttcaacgaca
     2521 atttcgggca cggcgtcggc gaccaggtgc ttcggttgat gtcgaacgtg ctgcgggagc
     2581 ggcttcggga catcgatctg ccggctcgat atggcgggga agaactcatc gccgtgctcc
     2641 cgggtgccga cctcccaggt tgcgaggcgg ttgccgagcg catacgccgc gcaatctcgg
     2701 agtgtcgcat cacccgtcgc tcgaccgggg agattcttcc cagcatcacg gtttccatcg
     2761 gggtcggaca gtttcagttc ggcgagtcga tggcggattt gatcgagcga tgcgatcgcg
     2821 cgctatatct cgccaagaga accggacgcg accgggtcgt gagcgagaat gaactcgatc
     2881 gccagctgga ggcgagctag ctcgccagcg ccactgtgcc ggcgccatcc gtgattcgca
     2941 caaagccatg ctcgaccacg gcgttgcggc cgccgtgttt ggcggcatag agcgcggcgt
     3001 cggcggcctc gatcaggtcg cccggccgct gggcgtcgtt cggtttggtg caggcgaccc
     3061 cgacgctgac ggcttggtag aggctggtgg cgtggggcat cgcgagggcc tggaccgccg
     3121 cgcgcaccgt ctcgccgatc tccaacgcgc ggactgcgtc ggtattcggc agcagcagac
     3181 agaattcctc gccgccatag cgcccggcaa aacccatggt ctcggcggca attcccgcca
     3241 gcgtctcgcc gagcctggag aggcagacgt cgccttcggg atggccatag gtgtcgttga
     3301 acagcttgaa atgatcgacg tcgatcatca gcagcgacag cgcggtgtca tattgctggg
     3361 acttcatcca ttcgaagtcg agccggctct ggaagccgcg ccggttggca aggccggaga
     3421 gcatgtcgat cgaggcgatc acggtcagcc ggtcattggt agcgaccagc tcgcgttcgc
     3481 gttcggcaag ccgtgccgcc atcgcgttga gtgcgcgggc cagcgggata aattccgacg
     3541 gcaagcggcc gcgtgccgcg cgcaccgacc agttgccttg gccgaagcgt ttggccatcg
     3601 ctgccatcat ctcgatcggc tggatgatga gcttttccgc ggcgatcaat gcgccgagca
     3661 gcacgaagag gctgacgaac cccagctgca gataggcggt gcgaatatcg cggtcgatag
     3721 cggcggaaac cttggtctcg tcgatgctga cgatcaggcg tgacccagtg ccggcaatgc
     3781 gcgcaaaact gaccgcgcgc ctcgagccat ccacggcgac gaacgaaagc gttccttccg
     3841 cctggtccga atcgatggcc tttgcggcga tggctgatag caatggtact gcatccagcg
     3901 accggccgat catgctggcc tgatcggccg gcgccgccag cacggttccc gtgctatcga
     3961 tcagaaccgc tgaaacgccg ggccggccgc cgagattgct catgattttc gacatccagt
     4021 cgagattgac gccggcaaga ataattgaat ccacatcgtt attgatggcg aagaccggat
     4081 aagccgccat cacgactggc ttgttggtaa gcttggcgaa cagaaagtcg ctgaagacaa
     4141 tctcgtggct ctctcgcgcc ctcttgaggt aggcccggtc gctgagatcg acaccgacaa
     4201 agatgtcgtt tgtcgagcat tgaacgcgtc cgtccccgcc aacaaccagc aaagtgcgga
     4261 tccacggcag attgaccggc aggctggcgc gcaggatgtc gcaactacga tcgacgccgc
     4321 cagccgaggc gcggatatag gccgccgatt tcagcactgt ctcgaccgag gagatcattt
     4381 cgcgctgcgc atcagcgcta tgctgcgcga gactcgagaa ttcctgggag gctagcgcga
     4441 tctgcttgct gcgggcgttc tcaagcgaac gaacgcgttc cagcatcaat ggggccacca
     4501 ggatcagcgc cagcaacgcc agtcgcgcgc gaattccaag aagcttcttg agtttcaccc
     4561 ttttgcggtt gagagtaacc cgtgacatcg tcgtcccccg gccccacatg cagggtagag
     4621 agaggggttc aagaagcctt tcccgaattt ggtaaaattc gaacgatccc gtaaacctac
     4681 gaccgataga atttcatgac gtccgaaact ccatcgccaa taactccgca tttaccaaac
     4741 gggctgctcg cggtggagca agacatcgcg cgcgcgtgcc agcaggcgcg ccgggatcgc
     4801 tcgtcggtga cgctggtcgc ggtatccaag accttcgatg ccggcgcgat ttcacccgtg
     4861 atcgaggccg gacagcgcgt gttcggcgaa aatcgcgtgc aggaagccaa gacgaagtgg
     4921 ccagggttaa tatcggctta ccccgatctc gccttgcacc tgatcgggcc gctgcaatcc
     4981 aacaaggcca gggaggccgt gacgctgttc gacgcgatcc attcggtcga ccgtccgagc
     5041 atttgcgaag cattagccaa agaaatcttt tctcaaaaac gcgcgccgca attgttcgtc
     5101 cagctcaata ccggcgagga gccgcagaag gcgggcgtcg cgccggccga ggcggacgcc
     5161 ttcattgcca gctgccgcga aaaatacggg ttggcaattt ccggcctgat gtgcataccg
     5221 ccggtcgacg atgcgccggc gccgcatttc gcgctgaccg cgaagatcgc ggcgcgtaac
     5281 gggctgaaga acctctcgat gggcatgagc gccgattttg ccattgcgat ccagttcggc
     5341 gcgacgcatg tgcgcgtggg ctcggcgatt ttcgggaagc gctagacctt ggcacgtgcg
     5401 gacgtcatcc tgagagcgtg agaatctttt ggcaatgagc atttgccaca gcggcagtgc
     5461 tgtgattcgc tcgcattgcg cgcgaagcag ggggctgacg atggctgacg agcaattgtt
     5521 cgaaggttta cccgagcagg aaa
//
LOCUS       DASWGM010000298        14510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_989553,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000298 DASWGM010000000
VERSION     DASWGM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14510
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989553"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VGA15_08645"
     CDS             complement(<1..1339)
                     /locus_tag="VGA15_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587799.1"
                     /translation="MAHVEFLDETMRDGQQSLWGMRMRAGMALPVSPIIDRTGFRVID
                     LAGSSMMEVLIRHCRENPWEGLDLLVQSMPRTPIRGGMRSNASVTFGVTPDSLMDAWM
                     RQLNRHGVRSFWIYDVLFNIDKTHRLAKVAKEFGSEVAGAIMYTLSPVHTDEYYADKA
                     DKLSVSPDIDTLLLYDTAGTLDKERLTTLVPAIVAKARGKKIEFHSNNILGMSAKAYL
                     DSIELGVSILHTASRPLANGPSVPSTEIMVKNIELKGHTHNLDISLLKPVADHFEAIG
                     KAAGYLVNQYSEYDILSIEHQIPGGMTGTLKAQLAQHNMSEKLDQVLTEAARVRRELG
                     YPGMATPFSQLVGTLAVLNIVSGKRYAIVPDEVIQYAAGFYGQTVAPIDPNVLDMIMS
                     APRAKEVLANPPEQPTIEELRKRYGTTNDDELILRALVPEADLDRMWAAGPVKT"
     gene            complement(1350..2711)
                     /locus_tag="VGA15_08650"
     CDS             complement(1350..2711)
                     /locus_tag="VGA15_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9587800.1"
                     /translation="MALNRLFIANRGEIAVRILRTAKRLGLETVIGVSEADRESMGAE
                     MADRAVVLGPAPSAKSYLDERLIVHAARMTGCDALHPGYGFLSEKAKLASLCEEEGIV
                     FVGPRPETIDMLGDKLSARTMALQAGVQTVPGTDHVAKIADAKHAAEQLGYPVVMKAS
                     AGGGGRGMFMARSAEELDISFERASREAETAFGDSRLYMERFVERARHVEVQIVGDGE
                     GTIVHFGERDCTVQRRYQKLIEEATSTAIPIRLRTKLHEAAIRITSFAKYRNAGTVEF
                     LYDVDREDFYFIEVNSRIQVEHPVSEEVTGEDLIARQLNVAAGQGIGLSQADVHFRGH
                     AIECRINAEDPFNNFMPAPGRITAWTPPSGEGIRLDTHARMGYLVPPFYDSMIGKLIA
                     KGRDRSEAIERILKAIRDFRLEGPKTTLPLISFVAAHSDFRENRITTRWLEDKGLPDF
                     ARA"
     gene            complement(2714..3220)
                     /locus_tag="VGA15_08655"
     CDS             complement(2714..3220)
                     /locus_tag="VGA15_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX9587801.1"
                     /translation="MTAVVKPEDIESLIELFEASDWDEMYLEIEGLELFLSSDPKARI
                     SAQPQHPTATHASASAHAHATTPQGMGHLAPPPTPAKEVSSAPEGCIAVKAPNLGTFY
                     RAPKPGAASYVELGQSVTADMEICLIEVMKLFTSVRAGTAGVIREICIDDAEMVEYGQ
                     TLFYIEPA"
     gene            complement(3289..5028)
                     /locus_tag="VGA15_08660"
     CDS             complement(3289..5028)
                     /locus_tag="VGA15_08660"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX9587802.1"
                     /translation="MGSNRHRWVISLDGSVLPDRSLIGGKAWSIARMMSLGLPVPPAF
                     VVTTEACKAFLADGAFPAELEAEISDGIAGLEKRTGRSFGKGDRPLLVSVRSGAPVSM
                     PGMMDTILNLGITPDTERALVVECGDPAFARDVHRRFFDLFAHIVLKCGVEDFSKEGN
                     AQSWNRQVAVATGSPLPCTAPERLLAAVRAVFDSWNSRRAKRYREHHGIPHDLGTAVT
                     VQAMVFGNLDDRSGTGVLFSRNPSTGDHKPFGEYLARAQGEDIVSGKFTPDTLDTMKT
                     SVPAAYEALMASSAMLERENGDVQDIEFTVQKGELFLLQSRSAKRAPAAAIRIAIDMA
                     REGAISEETALDRITPEQVRLILAPRLADGQSDNAKVIACGEGACPGVGRGIVVTSSD
                     EAEARASTGEAVILARPTTSPDDVHGMLAAKAIITEAGGSTSHAAVVSRALGLPCVVG
                     CGSGSLEFLSGRIVTVDGALGRIFEGALDVVTPDETSDERLTVLTRWAKARSRLIVHL
                     PSEAPSEDLIDLNTVEGGEDLKRLPGLIRGYRGARGGAIASDEGIRAALQEKLEFVVG
                     EPVLPLLIAAIRS"
     gene            complement(5040..5642)
                     /locus_tag="VGA15_08665"
     CDS             complement(5040..5642)
                     /locus_tag="VGA15_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587803.1"
                     /translation="MSDMHLALHGLAIKKHASPKDIAEIVGMDAERVASLLAEAESRG
                     RVARAGGKFVLTAPAQVALRAEYSRVYAEQRSSAAMNAAYDDFEKVNVLLKQLITDWQ
                     TMDVGGEKLPNDHSNKDRDEKIIDRLGDLHERSEPILKRMAASLPRLCIYLQMLTAAL
                     ERAEDGAIEWVSDARIASYHTVWFEMHEDLLRALGRERVE"
     gene            complement(5644..7104)
                     /locus_tag="VGA15_08670"
     CDS             complement(5644..7104)
                     /locus_tag="VGA15_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9587804.1"
                     /translation="MHFGDAPCLSEGGRTISFREFDAETNRLGNALLDAGLVPGDRVG
                     VLLPNSIECLVAYYALAKSGLVRVAMNTRETIENHRFKIEDSASRGVIHNGISDLGVE
                     IEIGPDALVAMIRDGRAAACAIDRALDAPLRLGYTGGTTGKSKAVTLTTRGELAELTA
                     FLTDLIPDIKQGDTFLHAAPVAHASGAFFLPALARGARSLIMGKFNPAEFLALAESEK
                     ASLTFLVPTMLAMIMEEPELEKSKLNFRRIAYGASPISPTLLQRAEKRFGRVFAQSYG
                     QAESPMVITCLKPEDHDRIGSCGRPFTMVDVAIFDDDDKPQQPGEKGEIVCRGPQTMA
                     HYWNRPEQTAEVFRNGWLHTGDIGWMDRDGFFYILDRKNDMLISGGYNVYPGEVEDVL
                     LAFPGVVEAAVIGLPDERWGDRVHAVVSGRPGLAGAALLDHARASLANYKCPKGIEIW
                     SELPKSSANKILRRTVRDRVIAAGKAGTEIRKEVIS"
     gene            complement(7213..8769)
                     /locus_tag="VGA15_08675"
     CDS             complement(7213..8769)
                     /locus_tag="VGA15_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587805.1"
                     /translation="MPQNRISYAQLLETNNLIQTCSDETYWLCVTRTVQESKLFPVPA
                     YMMLSYLMAFYRYPSLLRKIESRMRAEEVGDRARNMGMKIQNPSMGWALPGFYLLGRE
                     WLINMGLLKPTDAIEDVIYVMDFWKRFQLSWHRNDGHITNKEFGHRAQILPESRLQVF
                     HADMYDCKYGDELHQAAQAFMASASQYGFLISCESRISLHNSGPYKLADDRELLIRDF
                     MDLSESDFPWLDDVAKGVPHNNITVTMAVKDCHFYLVDDWGSFESQPGFKAEQLVGVG
                     LYTSDNLSDGYMPLGMDSREELIKTFKSLNDKIKDATTQLWKRIAGWRRDQMIDAGAI
                     TYFAIIKDLAHVAGVYDVDDWMKIDERAERFRPLLNDEFSRDALGELVGLVSHPSQQV
                     MEYTMAQHSNKPNRMYTSIPYSILKGEPYTVTSGPVYPGVTFLPEKKDAYNTTRGTIT
                     LAEYNKLSQEIVPPLLAEKYRYLCDNWVKYHAGTPLADDLYKADQTHSRRLKGKGSML
                     KRADIEKLRG"
     gene            complement(8818..9567)
                     /locus_tag="VGA15_08680"
     CDS             complement(8818..9567)
                     /locus_tag="VGA15_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX9587806.1"
                     /translation="MARLYVDSDKFGYQAASPTVEAFHSSIKDIVRAKLAKSGGDWDS
                     FVFDKSYDLVTADDMRSVEKLLLGAGHKFDWSAAISPRERPDIYKSAAGTSDADLKTF
                     SFEHADAQTAPADKNGRELRGIGDNVAKFPKNTVGIAHYIRSPDQVLKYLTDGVPADA
                     IAVIDDSGGTLTAPILEHFKGVICAGGSTRSHLGILTREYGVPCLMNAKISGIRNGDT
                     VEIEVTAAAKTAEAYQKGIEMTANIWRLKSA"
     gene            9814..10503
                     /gene="surE"
                     /locus_tag="VGA15_08685"
     CDS             9814..10503
                     /gene="surE"
                     /locus_tag="VGA15_08685"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX9587807.1"
                     /translation="MVRQFTEDVWVVAPDEEKSGASHSISMNIPVRVREIDSRHFAIK
                     GTPTDCALLAIYEIMKMRPTILLSGINRGANLAEDITYSGTAAAAMEGALLGVPSVAL
                     SQVFTPRGQVPWETAEKYTPLILERLLAAEWQPNAFVNVNFPNTAPDAVSGIRVVSQG
                     QRPPGSFLPEGRIDGRGVPYYWIKLAYKDGGFEPGSDLEAIRDDAVSVNPIQLDMTAN
                     PFRERLACSFT"
     gene            complement(10516..11259)
                     /locus_tag="VGA15_08690"
     CDS             complement(10516..11259)
                     /locus_tag="VGA15_08690"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX9587808.1"
                     /translation="MNRRRIEFEGAVNFRDLGGYPAEGGRSTRWRTIYRSDSLADLTE
                     SDVCDLQQLGIKTLCDFRLSYESERKPNRLPNGHRINVVLLPFIPEGTLDMLSAINRG
                     QYGPADVEREVLDHYKKFVNDHASEYRRVFEVILHKDSLPLLMHCTSGKDRTGFAAAA
                     VLLAVGVSREIIVRDYALTNDFRRDIRHLFSNAVSKPTIDVLTSANPRYLEAALDEID
                     ARFGTTEAWLESLGLNPSRRARLRDLLTE"
     gene            11409..12527
                     /locus_tag="VGA15_08695"
     CDS             11409..12527
                     /locus_tag="VGA15_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9587809.1"
                     /translation="MPARNQRGGLHLARHAWAWKWRRRPEAACCVQKIKKMTDHIQGG
                     KNMAARKLIFIVSAAALALSTAASFAADKPKVGIVAVNQNSPTINRMVVTATEAAKAK
                     GWTIEGFDGQGDRVATNNAAMGFIDRGFNAIINIASPNPQMTAVINRAKEKNIPFVST
                     FSGLAPNITADIGSNNTADGVISATELIGRLGGKGHIVKLNWNVLPALRERDHGFKAV
                     IVDYPEIKVTEIEVKVPGQVDDTYNRVTNLLLANPDINAIWTGWDELVAPSVRAIEQA
                     GKQNSIIVVSQDGIPEVFDQIASGGPAKLTVAYDVDRKGTTAVDVTAAALDGKMPASK
                     LLTLAPCLVTKDTAPAKGERPKFGFCVLFSGEALAAKK"
     gene            12602..14170
                     /locus_tag="VGA15_08700"
     CDS             12602..14170
                     /locus_tag="VGA15_08700"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX9587810.1"
                     /translation="MALTETVTSIPGPAYDLAVPVLEVRGLTKEYPGTRALDSVSLHV
                     GHGEIHALLGENGAGKSTLIKCVVGAQRPTAGDILVDGKAVEFSGPYEAVAAGIAVVH
                     QHSNLIPSLAVEENIWLGEPLPRIAGTLVNWRAVRTRAVEIMNYVGLDIPPDAYVGEL
                     RPDQRAMISIAKAIASKARLIILDEPTTALLPHEVDMLFGQMRRLSREGHAFLYVSHR
                     LTEVFNIADRATVLRDGRNAGTFERAAMDRHAVISAIVGSQKSLRKGRPAAVTRERVV
                     MTVDGLSGSRVDTVGFDLRQGEILGIAGLPGSGADETIDLLFGRTHRSGGTISVDGQP
                     IRLRHPKDAISAGIVLVPKDRLAEAIIHGYSVRENITIPSLAKYVRDPIARFINRGAE
                     DRAAKDVVERMRVRARSIETPIESLSGGNQQKAVLGRWLTTGARIFLLNSPTAAVDVG
                     AKAEIYELIRTLASEGAAVIFSSTEVEEFPALCERVLIFSGGRIAGELTGDSVTEANI
                     MTIAVGGAIGGHRD"
     gene            14151..>14510
                     /locus_tag="VGA15_08705"
     CDS             14151..>14510
                     /locus_tag="VGA15_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587811.1"
                     /translation="MEATGIDVTEGSRTARFDMVAFLARYGTILSLVLLLAIFSFARP
                     EVFPSVRNLLNILNQVSILGIIAIGLTVCLVIGQFDLSIGALATFGGYFATRILMETG
                     IDAGYAVSLVCGLVLILA"
BASE COUNT         2818 a         4393 c         4342 g         2957 t
ORIGIN      
        1 tcgtctttac cggacccgcc gcccacatgc gatcgagatc ggcttcaggc actaacgcgc
       61 ggaggatcag ctcatcgtcg ttggtcgtac cgtaacgctt gcgcaattcc tcgatggtcg
      121 gttgctcggg cggattagcc aaaacctcct tggcgcgggg cgctgacata atcatgtcga
      181 gcacgtttgg atcaatcggg gccaccgttt gaccgtaaaa gcccgcagca tactggatca
      241 cttcgtcggg cacgatcgca taacgcttgc cggaaacgat gttgagcacg gccagtgtgc
      301 cgacgagctg gctgaacggc gttgccatgc cgggatagcc gagctcgcgt cggacgcggg
      361 cggcttcggt cagcacctgg tcgagctttt cgctcatatt gtgctgggcg agttgtgcct
      421 tcagcgtgcc ggtcatcccg cccgggatct gatgctcgat ggagaggatg tcgtactcgc
      481 tgtactggtt gacgagatag ccggccgctt ttccgatcgc ttcgaaatga tcggcaactg
      541 gcttcaacaa actgatgtcg aggttgtggg tatggccctt cagctcgatg ttcttgacca
      601 taatctcggt cgagggaaca gaaggaccat tggccaacgg gcggctcgcg gtatgcaaaa
      661 tcgaaacgcc aagctcgatg gaatcaagat aggccttggc cgacatgccg aggatgttgt
      721 tggaatggaa ctcaatcttc ttgccgcgcg ctttggcgac gatggccgga acaagcgtcg
      781 tgagccgctc cttgtctagc gttcccgccg tatcgtagag cagcaatgtg tcgatgtcgg
      841 gcgagaccga cagcttgtcg gccttgtcgg cgtaatattc gtcggtgtgg acgggcgata
      901 acgtgtacat gatcgcgccg gcgacttccg agccgaactc cttggcgacc ttcgcaaggc
      961 gatgcgtctt gtcgatattg aacaacacgt catagatcca gaacgaacga acgccgtggc
     1021 ggttgagttg ccgcatccag gcatccatta gcgaatccgg cgtcacgccg aatgtcaccg
     1081 acgcgttgga tcgcatgccc ccgcggatcg gcgtgcgcgg catcgactgc accagaagat
     1141 ccagtccttc ccacgggttc tcgcggcaat ggcgtatcag cacttccatc atcgaggaac
     1201 cggccaggtc gatgacacgg aaaccggtgc ggtcaatgat cggagacacc ggaagtgcca
     1261 tgcctgcgcg catccgcatg ccccacaggc tctgctgtcc gtcgcgcatc gtctcgtcga
     1321 gaaactcaac atgggccatg gccgaactcc taagctctag caaagtctgg aagacccttg
     1381 tcctccagcc agcgcgtggt gatccggttt tcccggaaat cgctatgcgc cgctacgaag
     1441 gaaatcagcg gcagggttgt cttggggcct tccaggcgga aatcgcggat ggccttcaaa
     1501 atgcgctcga tagcctcgct acggtcccga cctttggcaa tcagcttgcc gatcatggaa
     1561 tcgtagaatg ggggcaccaa ataacccatg cgggcgtggg tatcgagccg gatgccctcg
     1621 ccagaaggcg gcgtccaggc ggttatgcgg cctggagcag gcatgaagtt gttgaacgga
     1681 tcttcagcgt tgatccgaca ttcgatggcg tggccacgga aatggacgtc tgcttgcgaa
     1741 agcccgatcc cctgacccgc cgcgacgttc aactgccgcg cgatcagatc ttccccggtc
     1801 acttcctcgc tgacgggatg ctccacttgg atgcgcgaat tcacttcgat gaaatagaaa
     1861 tcctcccgat cgacgtcata gaggaattcg accgttccgg cgttcctgta cttcgcgaag
     1921 gacgtgatcc tgatcgccgc ttcatgcagt tttgtgcgca gacggatggg tatcgcagtc
     1981 gacgtcgctt cctcgatcag tttctgatag cgccgttgca cagtgcagtc gcgctcgccg
     2041 aaatgaacga tcgttccctc tccgtccccg acgatctgga cctcgacatg gcgcgcacgt
     2101 tcgacaaaac gctccatgta aagccggctg tcgccgaacg cggtttcggc ctcgcgcgat
     2161 gcgcgctcga aggagatatc gagctcttca gccgaacgtg ccatgaacat gccgcgtccg
     2221 ccaccgccgg ccgaagcctt catgacgaca ggatagccga gctgttcggc tgcgtgcttg
     2281 gcgtcggcaa ttttggcgac atggtccgtg cccggaacag tctgcacgcc cgcttgcagc
     2341 gccatggtgc gtgccgacag cttgtcgccc agcatatcga tggtttcggg ccgtggcccg
     2401 acgaaaacga tgccctcctc ctcgcagagc gaagcaagtt tcgccttctc ggaaagaaat
     2461 ccatagccgg gatgaagagc atcgcagccg gtcatcctcg cggcgtgaac gatcaggcgt
     2521 tcatcaagat agctcttggc cgagggtgcc ggcccgagca cgacggcgcg gtccgccatc
     2581 tctgcgccca tgctttcacg atcggcctcg gagactccga tgacggtctc gagaccaaga
     2641 cgttttgcgg tgcgcaggat gcgcacggcg atctcgcccc tgttcgcaat aaagagtcgg
     2701 ttcagtgcca tggtcaggcc ggttcgatgt agaacagcgt ctggccgtac tcgaccattt
     2761 cggcatcgtc gatgcagatc tcgcgaatga cgccggctgt tcccgcacgc acggaggtga
     2821 acagcttcat cacctcgatc agacagattt ccatatcggc cgttacgctc tggccgagtt
     2881 cgacatagga cgccgcgccc ggcttcggcg cgcggtagaa cgtaccgaga ttgggcgcct
     2941 tcaccgcgat gcatccctcc ggcgcgcttg acacttcctt ggccggagtc ggcggaggtg
     3001 cgagatggcc catgccctgc ggggtcgttg catgcgcgtg agcagaagcc gaagcatgcg
     3061 tcgcagtcgg atgttgcggc tgcgccgaga tccgggcctt cggatcggag gacaggaaga
     3121 gttcgagccc ctcgatctcg agatacatct cgtcccaatc agacgcctcg aacagctcga
     3181 tcagtgattc gatatcttca ggtttcacga ccgctgtcat tttcttctcg ttttccgtca
     3241 tgaactagca gtctccgcag ccagcatttc tccggctgct tttttggtct acgaacggat
     3301 ggcggcgatg aggagcggca gaaccggctc accaacgacg aattccagtt tctcttgcag
     3361 cgccgcccgg attccttcgt cgctggcaat ggccccacct cgcgcgccac gatacccgcg
     3421 gatcaggccg ggtagccgtt ttagatcctc gccaccttcg accgtgttga ggtcgatcaa
     3481 atcttccgaa ggcgcttccg atggaaggtg aacaatgagc cggcttctgg ctttcgccca
     3541 tcgggtcagc accgtgagcc gctcgtcgga cgtctcgtcc ggagtcacca catcaagcgc
     3601 gccttcgaag atccggccaa gcgcgccatc gacggtgacg atccggcccg aaaggaattc
     3661 aagactgccc gagccgcagc cgaccacgca gggtagaccg agcgcccgac tgacgaccgc
     3721 ggcgtgcgag gttgagccgc ccgcctcggt gatgatcgcc ttggcggcaa gcatgccgtg
     3781 cacgtcatcg ggactggttg tcggccgagc cagaatgacg gcctcaccgg tgctcgctcg
     3841 cgcctcggct tcgtcggaac tggtcacgac gatgccgcgt ccgacgcccg ggcatgcgcc
     3901 ttcgccacac gcgatcacct tggcattgtc cgactgtccg tcggccagac gcggcgccag
     3961 gatcagccgc acctgttcgg gcgtgatgcg gtcgagcgcg gtttcctcgc taattgcacc
     4021 ttcgcgcgcc atgtcgatgg cgatgcggat cgcggcggca ggcgcccgtt tggccgaacg
     4081 gctctgcagc aggaaaagct cgcccttctg caccgtgaac tcgatatcct ggacatcgcc
     4141 gttctcgcgt tcaagcatcg ccgaagacgc catcagggct tcgtaagccg cggggacgct
     4201 ggtcttcatc gtgtccagcg tatcgggggt aaacttgccc gagacgatat cttcgccctg
     4261 cgcgcgggcc agatactcgc caaacggttt gtgatcgccc gtcgacgggt tacggctgaa
     4321 cagcactccc gtgccgctgc ggtcatccag attgccgaac accatggcct gcacggtcac
     4381 ggcggttccc agatcgtgtg gaatgccgtg atgctcgcga taacgcttgg ctcggcgcga
     4441 gttccaggaa tcgaacacgg cgcgcaccgc ggccagcaga cgctcgggtg ccgtgcaagg
     4501 cagcggcgag cccgtagcta ccgcgacctg cctattccag ctttgcgcgt tgccttcctt
     4561 ggagaaatcc tcgacaccgc acttgagcac gatatgcgca aaaagatcga agaagcggcg
     4621 atggacatcg cgggcgaaag ccgggtcgcc gcattcgacg acgagtgccc tttcggtgtc
     4681 cggtgtgatg ccgagattga ggatcgtgtc catcataccg ggcatcgaga cgggcgcacc
     4741 ggagcgcaca gaaaccagca agggcctatc gcccttgcca aagctgcgtc cggtgcgctt
     4801 ttccaggccg gcgatgccgt cggatatctc ggcttccaat tcggccggaa acgcgccgtc
     4861 cgcgaggaac gccttgcaag cttcggtcgt gacgacgaaa gcaggcggca ccggcaggcc
     4921 aagcgacatc atgcgtgcga tcgaccaggc cttgccgccg atcagcgacc gatcgggcaa
     4981 cacgctgccg tccagactga tcacccatcg atgtctgttc gatcccatgc gacgaaatcc
     5041 tattcgacgc gttcacggcc gagcgcgcga agaaggtctt cgtgcatctc gaaccagacg
     5101 gtgtgatagc tggcgatgcg ggcgtcgctt acccattcga tcgcaccgtc ctcggccctt
     5161 tcgagcgcgg ccgtcagcat ctgcaaatat atgcaaaggc gcggtaggct cgccgccatc
     5221 cgcttcagga tcggttcgga gcgctcatgc agatcgccga gccggtcgat gatcttctca
     5281 tcgcggtcct tgttcgaatg gtcgtttggc agcttctcgc caccgacatc catcgtctgc
     5341 cagtcggtga tgagctgctt caacaagaca ttgaccttct cgaaatcgtc ataggctgca
     5401 ttcatcgcgg cgcttgagcg ctgctcggca tagacgcgcg aatattccgc gcgaagcgcc
     5461 acctgcgccg gcgcggtcag cacgaatttg ccaccggccc gcgccacgcg gccccgactt
     5521 tctgcctcgg ccaacagcga cgccaccctc tcggcatcca tgccgacgat ctcggcaatg
     5581 tccttcggac tcgcatgctt ctttatggcc agcccatgga gggcgagatg catgtcgctc
     5641 atttcaggaa atcacctctt tacgtatttc tgtgccggcc ttgccggccg cgatgaccct
     5701 gtcgcggacg gttcgcctga gaatcttgtt ggccgaactc ttcggcagtt cggaccatat
     5761 ctcgatgccc ttggggcatt tgtaattggc aaggctcgcc cgcgcatgat ccagcagggc
     5821 ggcgccggcg aggcccggcc ggcctgagac gacggcatga acccgatcgc cccagcgctc
     5881 gtcgggaagg ccgatcaccg ccgcctcgac gacacccgga aaagccagaa gtacatcttc
     5941 cacttcgccc ggatagacat tgtacccacc cgagatcagc atgtcgttct tgcgatcgag
     6001 gatatagaag aagccatcac ggtccatcca gccgatgtcg cccgtatgca gccagccgtt
     6061 gcggaagacc tctgcggttt gctccgggcg gttccaataa tgggccatgg tctgcgggcc
     6121 acggcagacg atctcgccct tctcgccggg ctgctggggc ttgtcgtcat cgtcgaatat
     6181 ggccacgtcg accatcgtga acggtcgccc gcatgagccg atgcgatcat gatcttcggg
     6241 cttgaggcag gtgatgacca tcgggctttc ggcctgacca tagctttggg caaagacgcg
     6301 gccgaagcgt ttttccgcgc gctgaagaag agtgggcgaa atcggagacg cgccataggc
     6361 gatacgccga aaattgagtt ttgacttctc caactccggt tcttccatga tcatggcgag
     6421 catggtcggt accaggaagg tcaggctcgc cttctcgctc tcggcaagag cgaggaattc
     6481 ggccgggtta aatttgccca tgattaacga gcgtgcgcca cgtgcgagcg cgggcaaaaa
     6541 gaacgcaccg gatgcatgcg caacgggcgc ggcatgaagg aacgtgtcgc cctgtttgat
     6601 gtcgggtatg aggtcggtga gaaacgccgt cagttccgcc agctcgccgc gagtcgtcag
     6661 cgtcaccgcc ttcgacttgc cggttgtgcc gccagtgtag ccgaggcgga gcggcgcatc
     6721 gagcgcacgg tcgatcgcgc aggctgcggc acgcccatcg cggatcatcg cgacgagcgc
     6781 gtctgggcct atctcgatct caacgccgag atcggaaatg ccgttgtgga tgaccccgcg
     6841 tgatgcactg tcctcgatct tgaagcggtg gttctcgatc gtctcgcggg tgttcatggc
     6901 gacgcgcaca aggccggact tggccagcgc ataataggcc accagacact caatcgagtt
     6961 gggcagcagc acgcccacgc ggtcgccggg caccaggcct gcgtcgagga gcgcattgcc
     7021 gagccggtta gtctcagcgt cgaactcgcg gaagctgatc gttcttccgc cttcgctcaa
     7081 gcatggcgcg tcgccgaaat gcaaggctgc gcgcctgacg agtgatccga tttgcataga
     7141 tctcaatccg tatggcgctg acgcggtgga tgaccggcgg gaggcgaagc aggccccccg
     7201 cgcgcggcag aatcaaccgc gcagtttttc gatgtcggcc cgcttcagca tcgagccctt
     7261 acccttcagc ctgcgggaat gcgtctggtc cgctttgtac aggtcgtcgg ccaacggggt
     7321 gccggcgtgg tatttgaccc aattgtcaca gagataacgg tacttctcgg ccagcaaagg
     7381 cggcacaatt tcctggctga gcttgttata ttcggcgagc gtaatcgtgc cgcgcgtggt
     7441 gttgtaggcg tccttctttt caggaagaaa tgtgacgccg ggatagacgg gtccgctggt
     7501 caccgtatag ggctcgccct tgagtatcga atacggaatg ctggtgtaca tcctgttcgg
     7561 tttgttcgag tgctgcgcca tggtgtattc catgacctgc tggctcggat gggacacgag
     7621 gccaaccagt tcgccaagcg cgtcgcggct gaattcgtcg ttgagcagcg gacgaaagcg
     7681 ttctgcgcgt tcgtcgatct tcatccagtc atcgacatcg tagacgcccg cgacgtgggc
     7741 caaatccttg atgatggcga agtaggtgat cgcgccggca tcgatcatct gatcgcgcct
     7801 ccatccggcg atgcgcttcc agagctgcgt cgtcgcgtcc ttgatcttgt cgttgagcga
     7861 tttgaaggtt ttgatcagct cttcgcgcga gtccatacca agcggcatgt agccatctga
     7921 cagattatcg gacgtataga gcccgacgcc aactaattgc tcggccttga aacccggctg
     7981 cgattcgaac gagccccagt cgtcgaccag atagaaatga caatccttca ccgccattgt
     8041 cacggtgatg ttgttgtgcg ggacaccctt ggctacgtcg tcgagccaag ggaaatcgct
     8101 ctcggaaaga tccatgaagt cgcgaatgag cagctcgcgg tcgtcggcaa gcttgtacgg
     8161 gcctgaattg tgcagcgaga tccggctttc gcacgagatc agaaagccat actgggaagc
     8221 cgaggccatg aaggcctggg cggcttgatg caattcgtcg ccatacttgc agtcgtacat
     8281 gtcggcatgg aagacctgca ggcggctctc gggaaggatc tgagcacgat ggccgaattc
     8341 tttgttggtg atgtggccat cgttgcggtg ccacgacagc tggaaacgct tccagaagtc
     8401 catgacatag atgacatcct cgatcgcgtc tgttggcttg agcaatccca tgttgatcag
     8461 ccattcgcgc ccgagcaggt agaagccagg cagcgcccat cccatcgacg ggttctggat
     8521 cttcatgccc atgttacgag cgcggtcgcc gacctcttcg gcccgcatgc ggctctcgat
     8581 ctttcgcagc agcgacggat agcggtagaa ggccatcaga tagctgagca tcatgtaagc
     8641 gggaactgga aatagctttg attcctgcac agttcgcgtg acgcagagcc aataggtttc
     8701 gtccgaacag gtctgtatca gattgttggt ttccagaagc tgcgcataac ttatgcggtt
     8761 ctgcggcatg gttgttcctc ccgaagaaac agagcttgtc gttggcgttg ctcttaatca
     8821 cgcgctcttc aggcgccaga tattcgcggt catttcgatg cccttctgat aggcttcggc
     8881 ggtcttggcc gcggccgtga cctcaatctc cacggtatcg ccattgcgaa tgccgctaat
     8941 cttggcgttc atcaggcacg gcacgccgta ttcgcgggtt aaaatgccca gatgcgagcg
     9001 ggtcgaaccg ccggcgcaga tgacgccctt gaaatgctcg agaatcggtg ctgtcagcgt
     9061 gccgccggaa tcatcgatca cagcaatggc gtccgccggg acgccgtcgg tcaggtactt
     9121 caggacttgg tcgggtgaac ggatgtagtg tgcgatcccg accgtgttct tcgggaattt
     9181 cgcgacattg tcgccgatgc cgcgcaattc gcgtccgttc ttgtcggcag gcgcggtctg
     9241 cgcgtcggca tgctcgaacg agaacgtctt caggtcggca tccgaggtgc cggctgccga
     9301 cttgtagata tccggacgct cgcgcggcga aattgccgcc gaccagtcga acttgtgacc
     9361 agcaccgagc agcaacttct cgacgctacg catgtcgtcg gcggtgacca ggtcgtagga
     9421 tttgtcgaaa acgaaggaat cccagtcgcc gccggatttg gcgagtttcg cgcggacgat
     9481 gtctttgatc gagctgtgga atgcttcgac ggtcggcgac gccgcctgat agccgaattt
     9541 atcggaatcg acgtaaagcc ttgccacggc ctcctccctg gttttgctct tcttccccgg
     9601 ccgatctttg cagcggcctt gtcatcagac tgcaagggga ggcagccggt gtcaacctat
     9661 gttcatacaa aattgtcaaa caatcctatt ttcctgtatc gtgccgggaa aaagggggaa
     9721 gcagtctcga catggttcag ctttcaaaac accgcgtcct catcatcaat gatgacggca
     9781 tccacgcgac cggcattctt ttgctagaaa aagatcgtcc gccagttcac cgaggatgtc
     9841 tgggtcgttg cccccgacga ggagaaatcg ggcgcctcgc attcgatttc catgaacatc
     9901 ccggttcgcg tgcgcgaaat cgacagtcgg catttcgcga tcaagggcac gccgaccgac
     9961 tgcgcgcttc tcgcaatcta cgagatcatg aaaatgcgtc cgaccattct tctgtcgggc
    10021 atcaatcgcg gcgccaatct cgccgaagac atcacctatt cgggtacagc cgctgccgcg
    10081 atggaaggag ccctgctggg cgtgccgtcg gtggcattga gccaggtgtt cacgccgcgc
    10141 ggccaagtac cttgggaaac agcggagaaa tatacgccgc tcattctgga gcgcctgctt
    10201 gctgcggaat ggcagcctaa cgccttcgtc aacgtcaatt ttcccaacac cgcgcccgac
    10261 gccgttagcg gcattcgggt cgtgtcgcaa ggacagcgtc cgccgggctc gttccttccc
    10321 gaagggagaa tcgacggccg cggcgtgccc tactactgga tcaagcttgc ctacaaggat
    10381 ggcggcttcg agccgggttc tgatctggaa gcgatccggg acgatgccgt ttcggttaac
    10441 ccgatccagc tcgacatgac ggcgaaccct ttccgcgaac gacttgcatg cagtttcacc
    10501 tgacacggga tgggtctatt cggtcaggag atcgcgcaaa cgagcacgcc gggaagggtt
    10561 caggccgagg ctttccagcc aggcttccgt cgtgccgaac cgggcgtcga tctcgtcgag
    10621 agcagcttcg agatagcgcg gattggcgga ggtcagcaca tcgatggttg gtttcgagac
    10681 ggcgttcgag aagagatgcc ggatgtcccg ccgaaaatcg ttagtcagcg cgtagtccct
    10741 gacaatgatt tcgcgggaaa cgccaaccgc cagtagcacg gcagcggccg cgaagccggt
    10801 gcggtccttg ccgctggtgc aatgcataag gagtggcagg ctgtccttgt ggagtatgac
    10861 ctcaaagacg cggcgatatt cagacgcgtg gtcgttgacg aatttcttgt aatggtcaag
    10921 cacctcgcgt tcgacgtcgg ccgggccata ctggccgcgg ttgatcgccg atagcatgtc
    10981 gagcgtacct tccggaatga agggcagaag gacaacattg atcctgtgcc cgttcggaag
    11041 acggttgggc ttgcgctcgc tctcgtagga taagcggaag tcgcagagcg ttttgatgcc
    11101 aagttgctgc aaatcgcaga cgtcgctctc ggtcagatcg gcgagcgagt ccgaccggta
    11161 gatggtccgc cagcgcgtgc tacgcccccc ttctgccgga tagcccccca gatcgcgaaa
    11221 attgacggcc ccttcgaact cgattcggcg tcggttcatt tcggctcgcc tcctatccgt
    11281 tcttgccatg cagagaatac cccaaactcg aacgaagatt gttttacaat tttgttaggc
    11341 aagaatgttg acgcttacct gtcgataaag gacggaggcc ggcagcaccg acccaccgcc
    11401 cgaagggatt gccggcgcgc aaccaaaggg ggggtcttca cttggcgcgg cacgcgtggg
    11461 cctggaagtg gcgcaggcga ccagaggctg catgttgcgt gcaaaaaatc aaaaaaatga
    11521 ccgatcacat ccagggagga aaaaacatgg ctgctagaaa gcttatcttt atcgtgagcg
    11581 cggcagcgct tgcgctgtcg accgccgcga gcttcgccgc ggacaaaccc aaagtcggta
    11641 ttgtcgccgt caatcagaat tcgccgacga tcaatcgtat ggttgtgacc gcgaccgagg
    11701 ccgccaaggc caagggctgg accatcgaag gctttgacgg acagggtgat cgggtcgcca
    11761 ccaacaacgc cgcgatgggc ttcatagacc gcggcttcaa cgccatcatt aatatagctt
    11821 cgccgaaccc gcagatgacg gctgtcatca accgtgccaa ggaaaagaac atcccgttcg
    11881 tatcgacatt ctccggactc gcgccgaaca tcacggccga tatcggctcg aacaacacgg
    11941 ccgacggcgt gatttccgca accgagctca ttggccgcct gggtggcaaa ggtcacatcg
    12001 tcaagcttaa ctggaacgtg cttccagccc tgcgagaacg cgaccatggc ttcaaggctg
    12061 tcatcgtaga ttatcctgag ataaaggtca ccgagatcga ggttaaggtt cccggtcagg
    12121 tcgacgatac ctacaaccgt gtcaccaatc ttcttctggc caatccggat atcaacgcga
    12181 tctggacagg ctgggacgaa ctggtagcac cgtccgttcg cgccatcgaa caggccggca
    12241 agcagaacag catcatcgtg gttagccagg acggcatccc ggaagtgttc gaccagatcg
    12301 ccagcggtgg ccccgccaag ctgacggttg cctatgacgt ggatcgcaag ggaaccaccg
    12361 ctgtcgacgt gacggccgcg gccctcgatg gcaagatgcc tgcatccaag ctgctcacgc
    12421 tggccccgtg tctcgtcacc aaggacacgg caccggccaa gggcgagagg ccgaaattcg
    12481 gcttctgtgt cctcttctct ggcgaagctc tcgccgcgaa gaagtagtgt ttgctggaga
    12541 tctcgctcac gttccgtgcc gcggaacccg cttggacgtc taatgaaacg acggtaacgg
    12601 catggccctc actgaaactg ttacttccat cccgggaccg gcttacgatt tggccgtgcc
    12661 ggtgctcgaa gttcgcggtt tgaccaagga gtatcccggc acgcgcgcgc tcgatagcgt
    12721 cagtctccac gtcgggcatg gcgagatcca tgcacttttg ggcgaaaacg gcgccggcaa
    12781 atcaacgctg atcaaatgcg tcgttggcgc gcaacgtcca accgccggcg acatcctcgt
    12841 cgatggaaag gccgtggaat tttcgggccc gtatgaagca gtcgcggccg ggatcgcggt
    12901 cgtccaccag cattccaacc ttattcccag cctggcggta gaggaaaaca tctggcttgg
    12961 cgaacccctg ccccgcatcg ccggtacact ggtcaactgg cgcgcggtcc ggacgcgggc
    13021 ggttgagatc atgaactatg tcggtctcga cattccgcct gacgcatacg ttggtgaact
    13081 gcgccccgac cagcgcgcga tgatctcgat cgccaaagcc attgcgagca aggcgaggct
    13141 tatcattctc gacgaaccaa cgacggcgct gctcccgcat gaagtggaca tgcttttcgg
    13201 ccagatgcgc cggctgtcgc gcgaaggcca cgccttcctc tacgtcagtc accggctgac
    13261 cgaagtcttc aatattgccg atcgcgccac cgtgctgcgt gacgggcgca acgccggcac
    13321 cttcgagcgc gccgcgatgg accgtcacgc cgtgatctcc gcgatcgttg gttcgcagaa
    13381 atcgctgcgc aaaggccggc cggccgcagt gacaagggaa cgcgtcgtca tgaccgtcga
    13441 tggccttagc ggctcacgag tcgacacggt cgggttcgac ttgcggcagg gagaaattct
    13501 cggcattgcc ggccttccgg gaagcggggc ggacgaaacc attgatcttc tgttcggacg
    13561 cacccaccgc agcggcggca caattagcgt cgacggccag ccgatccgcc tgcgccaccc
    13621 caaggacgca atctcggccg gcattgtgct tgtgcccaag gaccgcctcg ccgaggcgat
    13681 cattcacggc tactcggtgc gcgagaatat tacaattcct tcgctggcta aatatgtccg
    13741 cgacccgatc gcgcgcttca tcaaccgggg cgccgaggat cgtgccgcca aagatgtcgt
    13801 cgagcgcatg cgcgtgcgcg cacgctctat cgaaacgcct attgaatcgc tctcgggcgg
    13861 caaccagcag aaggcggtgc ttggccgctg gctgaccacc ggcgccagga tattcctgct
    13921 gaattcgccg accgcggcgg tggatgttgg cgcgaaggcc gaaatctacg aactcatccg
    13981 caccctcgcg tccgaagggg ccgccgtcat cttctcctcg accgaggtcg aggaattccc
    14041 ggcgctgtgc gagcgcgttc tcatcttttc gggcggcagg atagccggtg agttgaccgg
    14101 agacagcgtc acggaagcta acatcatgac aattgcggta ggaggagcca ttggaggcca
    14161 ccgggattga cgtaaccgaa ggctcgcgaa ccgcccgctt cgacatggtc gcattccttg
    14221 cgcgctacgg gacgatactt tccttggtcc tgcttctagc gatcttcagc ttcgctcgtc
    14281 cggaagtgtt tccgagcgtg cgcaatttgc tcaatatcct aaaccaagtc tctatccttg
    14341 gcatcattgc gatcggtttg accgtctgtc tcgtcattgg ccagttcgat ctatccatcg
    14401 gcgcgctggc tacctttggc ggttatttcg ccacccgcat tctgatggaa accgggatcg
    14461 atgccgggta cgccgtttct ctcgtctgcg gccttgtgct gatcctggca
//
LOCUS       DASWGM010000299         2811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2386038,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000299 DASWGM010000000
VERSION     DASWGM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386038"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1194)
                     /locus_tag="VGA15_08710"
     CDS             complement(277..1194)
                     /locus_tag="VGA15_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587812.1"
                     /translation="MRRIAVVGALAPDDVEIVKRSAAFEQALAALGWTKDDTIAIDYR
                     WIGANPALTEKTAEKTAVEVVALAPDVLLASGNVVIATLSKATGTIPIVMVQVIDPVG
                     SGYVESMARPGGNITGFTQFEYSLAGKWLDLLTEIAPRTSRVAVLREPTRGPGIGQFA
                     VIQAMAPARGVEPIPINPSDVADMERRITAFASVPNGGMIATVGGGIATHRATIIATA
                     NTNHLPATYPYRYYAADGGLVSYGPDTVDQYRRAASYIDRILKGEKPANLPVQGPTKY
                     ELTINLKTAKTLGLTVPPTLLVRADEVIE"
     gene            complement(1573..2415)
                     /locus_tag="VGA15_08715"
     CDS             complement(1573..2415)
                     /locus_tag="VGA15_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEX9587813.1"
                     /translation="MRWCVSIGAVLVAGAMAAGDVAGIAAPGPKSQQADTKENGPSVD
                     VELVIAVDVSYSMDLDELAVQREGYAQAIVSKDFLQAMKTGPNRKVAVTYFEWSASSD
                     QKIIIPWRVVDGPESADAVAAEIMKTPVRRGSRTSISGAINFAMPLFEENPYRGLRRV
                     IDISGDGPNNNGAPVTGARAAALEKGITINGLPIMVKEPSYSMMDIDDLDLYYEDCVI
                     GGPGSFVVAIKDREKFKEAIRTKLMLEVAGRTPEQRIVPVAEKEPRVPCLIGEKIWQD
                     RWGR"
BASE COUNT          546 a          904 c          804 g          557 t
ORIGIN      
        1 cagattcccg cacctgtgac ggaccaatca tgcgccgcaa cgtcgtcagc caacacactc
       61 atcggcgtcg gttgccctca cgcacctccg tcgtgcgcaa acctccatag cgctcggcgc
      121 tcgcggcgcc accgcctgcc atttcccgcg ggttccttca ttgaaggctt tcggacgccg
      181 gccacggtgc aagccgcacc gctgcgatcg gccggcatcc gaaaccctca acacaaccgg
      241 tcgtgagcag atgcagacga cttgcgcaaa tgcggcctat tcgatcacct cgtcggcgcg
      301 gacgagtagc gtcggcggca cggtgagccc cagcgtcttc gctgttttga ggttgatggt
      361 cagttcatat ttggtcggcc cctgcactgg caggttggcc ggcttctccc ctttgaggat
      421 gcgatcgata tagctggctg cacgtcggta ctggtcgacc gtatcgggac cataggaaac
      481 gagaccgccg tcagcggcgt agtagcgata gggataagtc gccggtaagt ggttcgtgtt
      541 ggctgtggca ataattgtcg cgcggtgcgt ggcgatgcct cctcccaccg tcgcgatcat
      601 tcctccgttg ggaacgctcg cgaacgccgt tatccggcgc tccatgtcgg caacgtcgga
      661 agggttgatc ggaatgggct ccaccccccg tgcgggcgcc atcgcctgga tcaccgcaaa
      721 ctgcccgatc ccggggccgc gcgtcggctc gcgtagaaca gcgacgcgtg aagtgcgcgg
      781 cgctatctcc gtcagcaggt cgagccactt accagcgagg ctgtattcga actgtgtgaa
      841 gcctgtgata ttaccgcctg gcctcgccat gctctcgaca tagccggacc cgactggatc
      901 gataacctgc accatcacga ttggaatggt acccgtcgcc ttcgacagtg tcgcaatcac
      961 aacgttgccg gacgcgagga ggacgtcggg cgcgagtgcg accacctcga ctgccgtttt
     1021 ttctgcagtt ttttccgtga gcgctggatt agcgccgatc caacggtagt ctatcgcaat
     1081 ggtgtcgtcc ttcgtccaac ccaatgcagc aagggcctgc tcgaatgccg cgctgcgctt
     1141 gactatttcg acgtcatccg gggcgagcgc gcccaccacg gcgatgcgcc gcatcgcgcc
     1201 ggatcgccgc gcccgcgccg cgatcggcca agccgccgcg acacccaatc aaagtgacga
     1261 actcgcgacg cttcattcga ccacctcgtc ggcagtcatg aatcgagcca gggcctgcac
     1321 tcccgtgctt ccgaccgaaa ggatagcaca cctcggcagg ggcgtgagat tgctgcgctg
     1381 cgggatttca aaccagctta tgtcgcagat gggtcaaact gagaagaact cagtgtgagc
     1441 aaatgtcttc cgagttaccg ctgaaagcgg acatcgctca atacagccgg catccgaaac
     1501 cctcaacacg atcggacatg acaggtccga aagcgacgtg actggctttg ttgaacaaaa
     1561 cattttcgac gcctagcgtc cccagcgatc ttgccagatt ttctcgccga tgagacaggg
     1621 cacccgcggc tctttctcgg ccaccgggac gatccgctgc tccggcgtcc ggcccgcgac
     1681 ctcgagcatg agcttggtcc ggatcgcctc cttgaatttt tcgcggtcct tgattgccac
     1741 cacaaaggag cccggccctc caatcacgca atcctcgtaa tataaatcga ggtcatcgat
     1801 gtccatcatc gaataggacg gctctttcac catgatcggc aggccgttga tggtgatgcc
     1861 cttctccagc gccgcggcgc gcgcgccggt caccggcgcg ccattgttat tggggccatc
     1921 gccggaaata tcgatgacgc gccgcaaccc gcgatagggg ttttcttcga acagcggcat
     1981 tgcgaaattg atcgcgccag aaatcgaagt gcgcgagccc cggcgaaccg gcgttttcat
     2041 gatctcggcg gcgacggcgt cggccgattc cggaccgtcg accacccgcc aggggataat
     2101 gatcttctgg tcgctcgatg ccgaccactc gaaataggtc acggccacct ttctgttcgg
     2161 accggtcttc atcgcctgaa ggaaatcttt tgagacgata gcctgcgcgt agccctcgcg
     2221 ctgaacggca agttcgtcca aatccatcga ataggagaca tcgacggcga tcacgagctc
     2281 gacatcgacg gaaggcccat tttctttggt gtcggcctgt tgagatttgg ggcccggcgc
     2341 ggcaatgccc gcgacgtcac ctgcggccat cgcccccgcg acaagcaccg ccccgatcga
     2401 gacacaccag cgcatagctg ccctcccgtc gtttcacggg gatggtgaca cgcaagacgc
     2461 gccgcgcaag gcgggaacgt gcgttgccgt tcacattcgg gttagtcgcc gttacaggcc
     2521 gcccgctctg atcggatgtc cgagttgggt caaaatccga agaactcact atgagcacat
     2581 gttttccgct ttgccccgaa ctcggacatt gcccgacgca gtcggcactt cgcatttgtg
     2641 ccccacaagc agacataagc tcacttaatc gatcaactcg tctgctcgac cgagcaagga
     2701 gagcggaacg gttagaccga gcgccttcgc cgtcttgata ttgatcacta actcgaccag
     2761 cgtgggctgc taaaccggca agtcagcggg ctactcgcct ttgagaatgc g
//
LOCUS       DASWGM010000300         6387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1134564,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000300 DASWGM010000000
VERSION     DASWGM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134564"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VGA15_08720"
     CDS             complement(<1..251)
                     /locus_tag="VGA15_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX9587814.1"
                     /translation="MSAKEVKFGVDARDRMLRGVEILNNAVKVTLGPKGRNVVLDKSF
                     GAPRVTKDGVTVAKEIELEDKFENMGAQMVREVASKSADA"
     gene            complement(341..658)
                     /locus_tag="VGA15_08725"
     CDS             complement(341..658)
                     /locus_tag="VGA15_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9587815.1"
                     /translation="MKFRPLHDRVVVKRIDAEEKTAGGIIIPDSAKEKPSQGEITAVG
                     PGGRDEAGKLIPIDLKVGDRVLFGKWSGTEVKLDGEELLIMKESDIMGVLTDVPASKK
                     KAA"
     gene            1039..1323
                     /locus_tag="VGA15_08730"
     CDS             1039..1323
                     /locus_tag="VGA15_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HEX9587816.1"
                     /translation="MVSRVGVSEDFRKQVLGYGLTTAQILYRMPDHPSLLQTYVWQNY
                     DLFPNFPALKDFLSFWEQKLEGPLFAVTVAHSKLIKPAELRAVDGVFRLH"
     gene            1465..2040
                     /locus_tag="VGA15_08735"
     CDS             1465..2040
                     /locus_tag="VGA15_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9587817.1"
                     /translation="MARKKPTKKPTSRTAARSAAQKKSPARKSAAKSTARKHIKAKDR
                     KTDRKTKARGLPAAKPRARPKQRIAISHYRDEDFKADGLRAYAHYRDLGIAEASHGLA
                     QAHVIRLIGPCNPAEVSKLHFHDVEFQMVYVLKGWVKTYMEGQGETLMKEGSAWTQPP
                     RIKHLIMDYSDDVELLEVILPAEFKTVELAS"
     gene            2099..4018
                     /locus_tag="VGA15_08740"
     CDS             2099..4018
                     /locus_tag="VGA15_08740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX9587818.1"
                     /translation="MNSAYREDIDWLRAIAVLAVVAFHFEAPAVFGGFVGVDIFFVIS
                     GYLITGIIQAEVRSGTFSFARFYERRARRLLPALYVMVALTAIPSLHYLLSSERLEFF
                     RSVAAVVTFTSNFFFWLQSGYFDHAAVEKPLLHTWSLAVEEQFYLALPVVLWVLLRFL
                     RVGRIALAVALGAMALVSFALCLWLMHTERSANAFFMSPPRAWEFLIGGLVAAPGLPV
                     LRNALAQQIARGIALVLLAIPIFSLRPGPGFPGVNALLPCIGAAVFIWSGIGVATQIR
                     SPYSHHSVIRFFGQISYSLYLWHWPLFTFARFSKTSLVLDPLDKMELFALTVAISYLS
                     WRFVEQPFRLRTPAPANRGAFRMAGLATAVLLAGSAGGIILSQMPSGADRDAMMLDVY
                     NSYDVRAIYRAGSCFRYDGGVLDDARCLSAVADKQNVLLWGDSYAAQYYHGLDRTIDR
                     EHVNLLQATQAACMPTLNASAQGNASCRSFAGQTDALFHDRKPDLVILSADWLEYARP
                     PRFDGMIADLNQTIARINAMGVAVVLLGPGVQFRSRLPSMLMRAQLRHIDPLPQDFVL
                     PDIFGLDRMMKAALPAHEKFSYISVVDAICPARRCPLTIDGGIPLAWDHAHLTAEGSV
                     YVMESMMPMLGVKKF"
     gene            complement(4124..5374)
                     /locus_tag="VGA15_08745"
     CDS             complement(4124..5374)
                     /locus_tag="VGA15_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEX9587819.1"
                     /translation="MLQLQSAFGVLALLALAWAFGENRGAVSLRQAAIGLAVTFVTAL
                     TLIKLPFVAHAFGIINDAVGAISAASRAGTAFVFGYLGGGALPFDLIAPGADFILAFQ
                     ALPVVLVMSVLTTLLFYWRILPPVVRGMAWLLERTLGVGGAVGLSTAANIFLGMVEAP
                     LFIRPYLAQLTRSELFLVMTGGMAGIAGTVLVLYATLLAPLIPDAAAHFVIASVLGAP
                     AAILVSLIMVPETSDKRTGGALADPDMHAASTMDAIVKGTVAGLELLLNIVAMLIVLV
                     ALVYLANAILGLLPNVGGVGISLQRMLGYAMAPVCWLMGLPWPQAITAGSLMGVKTVL
                     NELIAYVDLSKLGPDALDPRSRLIMLYAMCGFANFGSLGIMIGGLGTMAPDRRDEINS
                     LGLKSIVSGTLTTCLIGAIVGVLT"
     gene            5567..>6387
                     /locus_tag="VGA15_08750"
     CDS             5567..>6387
                     /locus_tag="VGA15_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587820.1"
                     /translation="MISADQGSPLKQRSFLLFLLTRSFSRFSSQVAAVAIGWQIYDLT
                     GSAFDLGMVGLVQFLPTALLVFVAGHAADRYERKRVVQICQLAQALTALFLAWGAYSG
                     SLTALQIFIATGVLGTATAFESPAIAALLPLIVPPGALQRATALSSGSAQIATITGPA
                     LGGLAYAVAPGMPYGMMVVFWLSGAILTGAIRLAQLVVVRDSATPADLFAGVRFVRSN
                     SAILGTISLDLFAVLLGGATALLPIYARDILQTGPWGLGILRAAPAVGALVMTAVL"
BASE COUNT         1166 a         1965 c         1988 g         1268 t
ORIGIN      
        1 gcgtcggccg acttggaggc cacttcgcgc accatctggg cgcccatgtt ctcgaacttg
       61 tcctcaagct cgatttcctt ggcgacggtg acgccgtcct tggtgacgcg gggtgcgccg
      121 aacgacttgt cgagcacgac gttgcgaccc ttggggccga gcgtcacctt caccgcgttg
      181 ttgaggattt cgacgccgcg cagcatgcga tcgcgggcat cgacgccgaa tttgacttct
      241 ttagctgaca tatttggttt tccctgagtt gatctttggt ctctcaccct tcgagatgcg
      301 ccgtgcaggc gctcctcagg gtgagggcgt gcagcgctgc ttaagcggcc ttcttcttgg
      361 aagcggggac gtcagtgaga acgcccatga tgtcgctctc cttcatgatc aggagctcct
      421 cgccatcgag cttgacctcg gtgcccgacc atttgccgaa cagcacgcgg tcgccgacct
      481 tgaggtcgat cgggatcagc ttgccggctt cgtcacggcc gcccggaccg actgccgtga
      541 tttcgccctg cgagggcttt tccttggcac tgtccggaat gatgatgccg ccagctgtct
      601 tctcttcggc gtcgatgcgc ttgaccacaa cgcggtcgtg aagcggacgg aatttcatgc
      661 agtcctccta agtttttgaa gatgttctcg aattttgaat tttggcagtc aatgccagcg
      721 agtgccagcg cgaccgaact ggacataagc caaacttgtg cggggaacaa gggcttctag
      781 cagaaaactt ggcactcaaa tatgacgcct gccaaaatcg tccatcatca ttaattcagc
      841 ggaacagaca cggaaaccca gtattagcac gtgcggtaag ctgctgctaa cgcgacattt
      901 ttcaacagat cattaaacat ttaggcttgt gatgagttgg tatcgtgcgt cacatttctc
      961 tcatgtgagc gcatctccac cgggtggctc ccggttggca gccacttcgt gaaatgcgct
     1021 ccagcaatgg aggtaagcat ggtctcgcgg gttggggttt ccgaggattt ccggaagcag
     1081 gtgctgggtt atgggctgac gacggcacag attttatatc ggatgccgga tcacccctca
     1141 ctgctgcaga cctacgtctg gcaaaattac gatctgtttc cgaattttcc ggcgctgaag
     1201 gattttctct ccttctggga gcaaaagctc gaagggccgc tgttcgctgt caccgtcgcg
     1261 cattccaagc tgatcaagcc cgccgaactg cgcgccgtcg atggcgtgtt ccggctgcac
     1321 tgatcgaact ctgtagggtg ggctaaggcg cgcttgcgcc gtgcccaccg cgtcgttaag
     1381 cggtgggctc gctatcgctt agcccaccct acgaagcacg aattgtgcta acccttccga
     1441 acgagacgga aagggagaac gtcgatggcc aggaaaaagc cgacgaaaaa gccgacatcg
     1501 cggaccgcgg ctcgctccgc cgcgcagaaa aaatcgccgg cgcgcaaatc cgctgccaaa
     1561 tccaccgcgc gaaaacacat caaggccaag gatcgcaaga cggatcgcaa gaccaaggcg
     1621 cgggggctgc cggccgcaaa accgcgcgcg cggccgaagc aacgcatcgc catcagtcac
     1681 tatcgcgatg aggatttcaa agccgatggc ctgcgcgcct acgcgcacta tcgcgatctc
     1741 ggcatcgcgg aggccagcca cggcctggcg caggcgcatg tgatccggtt gatcgggccg
     1801 tgcaatccgg ccgaggtttc aaaactgcat ttccacgacg tcgagtttca gatggtctat
     1861 gtcctgaaag gctgggtcaa aacctacatg gaagggcagg gcgaaacctt gatgaaggag
     1921 ggcagcgcat ggacccagcc gccccggatc aagcatctga tcatggacta ttccgacgac
     1981 gtcgaattgc tcgaagtgat tctgccggct gagttcaaga cggtcgaact cgcttcatga
     2041 tctgcatagg tgttgttgca cgcgcgacgg gccgccggtc agaggcggcc cggccattgt
     2101 gaacagcgcc tatcgtgagg acatcgactg gctgcgcgcc atcgcggtac tcgcggtggt
     2161 cgccttccat ttcgaagcgc cggcggtgtt tggcggcttt gtcggcgtcg atatcttctt
     2221 cgtgatctcc ggttacctca tcaccgggat catccaggcg gaagtacgga gcggaacgtt
     2281 ctcctttgcg cgattctacg agcgccgggc acgccgcctg ctgcccgcgc tgtacgtgat
     2341 ggtggcgctg acggcgatac cgtccttgca ctatctgctc agctccgagc ggctggaatt
     2401 tttccgctcg gtcgccgcgg tcgtgacctt cacctcgaat ttcttcttct ggcttcaatc
     2461 cggctatttc gatcatgcgg cggtcgaaaa gccgctgctg catacctggt cgctggcggt
     2521 cgaggagcag ttctatctgg cgctgccggt ggtgctgtgg gtgctgttgc gctttttgcg
     2581 cgttgggcgc atcgcgctgg cggttgcgct cggcgcgatg gcgctggtct cgtttgcact
     2641 ctgcctctgg ctgatgcata ccgaacgttc cgccaatgca ttcttcatga gcccgccgcg
     2701 ggcgtgggag ttcctgatcg gcggactggt cgcggccccg ggcttgccgg tgctgcgcaa
     2761 cgcgctggcg caacaaatcg cgcgcggcat cgcgctggtg ctgctcgcca tcccgatatt
     2821 ctcgctgcgt ccgggaccgg gatttcccgg cgtcaacgcg ctgctgccgt gcatcggggc
     2881 tgcggtcttc atctggagcg gcatcggcgt tgcaacgcaa atccgaagcc cctattcgca
     2941 ccacagcgtc atcaggtttt tcgggcagat ttcctattcg ctgtatctct ggcactggcc
     3001 gctgttcacc tttgcgcggt tttcgaaaac cagcctggta ctggatccgc tcgacaagat
     3061 ggagctgttc gcgctgacgg tcgcgatctc atatctgtca tggcgatttg tcgaacagcc
     3121 gtttcgcctg agaacgccgg cgccggcaaa ccgcggcgcg tttcgcatgg cgggcctcgc
     3181 gacggcggtc ctgttggccg gcagcgcggg cggaatcatc ttgagccaga tgccgtcggg
     3241 tgccgaccgc gacgcgatga tgctggatgt ctacaacagt tacgacgtgc gggcgatcta
     3301 tcgcgcaggc tcctgcttcc gctatgacgg aggcgtcctc gacgatgcac gctgcctcag
     3361 cgcggttgcc gacaagcaaa acgtgctgct ttggggcgac agctatgcag cgcaatacta
     3421 tcacggcctc gaccgcacga tcgatcgaga gcacgtcaac ctgctgcagg cgacacaagc
     3481 ggcgtgcatg ccgacgctga acgcatcggc tcagggcaat gcgtcatgcc gcagctttgc
     3541 cgggcagacg gacgcattgt tccacgaccg caagcccgat ctcgtcattc tgtccgccga
     3601 ctggctggaa tatgcccgcc cgccaaggtt cgacggcatg atcgccgatc tcaaccagac
     3661 cattgcgcgg atcaacgcga tgggcgttgc cgtggtgctg ctcggcccag gcgtgcaatt
     3721 caggagccgg ctgccgtcca tgctgatgcg cgcgcagctg cggcatatcg atccgctacc
     3781 tcaggatttt gtgctgccgg atattttcgg gctcgaccga atgatgaagg cggcgctgcc
     3841 ggcgcacgag aagttttcct acatttcggt ggtcgatgcg atctgtccgg cgcggcgatg
     3901 cccgctcacg atcgacggcg gcattcccct ggcgtgggat cacgcgcatc tgaccgcgga
     3961 agggtcggtg tatgtgatgg aaagtatgat gccgatgctg ggggtgaaga aattttagct
     4021 tgtcattccg gggcagcccg cagggctgag cccggaatcc atagtcacga cagggagtat
     4081 ggattccggg cctgcgccaa gcggcgcgtc ccggaatgac aggtcacgtc agcactccca
     4141 cgatcgcacc gatcaggcag gtcgtcaggg tgcccgagac gatcgatttc aatccgagcg
     4201 aattgatctc gtcgcggcga tccggcgcca tggtgccgag gccgccgatc atgatgccga
     4261 ggctgccgaa attggcaaag ccgcacatcg catagagcat gatcagccgc gagcgcggat
     4321 cgagtgcatc gggaccaagc tttgataaat cgacataggc gatcagctcg ttgagcacgg
     4381 tcttgacgcc catcagactg cccgcggtga tcgcctgcgg ccacggcagg cccatcagcc
     4441 agcacaccgg cgccatggcg tagccgagca tgcgctgcag tgaaattccg acgccgccga
     4501 cattgggcaa cagtccgagg atggcgttgg cgagatatac cagcgccacc agcacgatca
     4561 gcatcgcgac gatgttgagc aggagttcga ggcccgcgac ggtgcccttg acgattgcat
     4621 ccatggtgga ggcggcgtgc atgtcgggat cggcaagtgc gccgccagtg cgtttatcgc
     4681 tggtctccgg caccatgatc aggctgacca ggatcgctgc cggcgcgccg agaaccgagg
     4741 caatgacaaa atgcgccgcg gcatccggaa tcagcggtgc cagcaaggtg gcgtagagca
     4801 ccagcacggt gccggcgatt ccagccatgc cgccggtcat caccagaaac aactcgctgc
     4861 gggtcagctg cgcaagataa ggccggatga acaagggtgc ttccaccatg ccgagaaaga
     4921 tgttggcggc ggtcgacagc ccgaccgcgc cgccgacccc gagcgtgcgc tccagcagcc
     4981 acgccatgcc gcgcacgacc ggcggcagga tgcgccagta gaacagcagc gtggtcaaca
     5041 cgctcatgac cagcaccacc ggcagcgcct gaaacgccag gataaaatcc gcgcccggcg
     5101 ctattaaatc gaacggtagc gcgccgccgc cgagatagcc gaacacgaag gccgtgcccg
     5161 cgcgcgaggc cgccgaaatg gcaccgacgg catcattgat gatgccgaag gcatgcgcga
     5221 cgaagggcag ttttatgagt gttagggctg taacgaaggt gacggcgagg ccgatagcgg
     5281 cttgtctgag cgaaaccgcg ccgcggtttt cgccaaacgc ccacgccagt gcgagcaggg
     5341 caagaacgcc gaacgccgat tgcaattgta gcattaatcc ccccgaggcc agcgcgatga
     5401 tgacggcggc acgctgcaag gcgcatgccg cgcgttttcg atcgggaggc ggccgaacgc
     5461 ccgacattga caaccggtca gggctcagcc acgcgtcact aatgacgtgc agcgggcgac
     5521 tgcccgccat tcgaaaaccg ggcagaggcc gttgaccata tcgaccatga tatcagcaga
     5581 ccagggctcg ccgctcaagc agcgatcttt tctgctcttt cttctcaccc gaagtttctc
     5641 gcggttctcc agtcaggtcg cggccgttgc catcggctgg cagatctacg acctgacggg
     5701 cagcgcgttc gacctcggga tggtggggct ggtgcagttt cttcccacgg cgctgctggt
     5761 tttcgtggcc ggccacgccg cggaccgcta cgagcgcaag cgtgtcgtgc agatctgcca
     5821 gcttgcccag gcgctgacgg cgttatttct cgcatggggc gcctattcgg gctcgttgac
     5881 ggcattgcag atctttatcg cgaccggcgt gctcggaacc gccaccgcgt tcgaaagccc
     5941 cgcgattgcg gcgctgctgc cgctgatcgt tccgccgggc gcgctgcagc gcgcgacagc
     6001 cttatccagc ggctcggctc aaatcgccac gatcaccgga cccgcgctcg gcggtctggc
     6061 ctatgccgtc gcgcccggca tgccctacgg catgatggtt gtgttctggc tatcgggggc
     6121 gatactgacc ggcgcgatcc gtctggcgca attggtcgtc gtcagggatt ccgcaacgcc
     6181 ggccgatctg ttcgccggcg tcagattcgt ccgcagcaat tccgccatcc tgggaacgat
     6241 ctcgctcgat ttgttcgcgg tgctgctggg cggtgcgacg gcgctgttgc cgatctatgc
     6301 gcgcgatatc ctgcagaccg gcccctgggg cctcggcatc ctgcgtgccg cgcccgcggt
     6361 cggcgcgctc gtgatgaccg ccgtcct
//
LOCUS       DASWGM010000301         3788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_158030,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000301 DASWGM010000000
VERSION     DASWGM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158030"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /gene="sucA"
                     /locus_tag="VGA15_08755"
     CDS             <1..460
                     /gene="sucA"
                     /locus_tag="VGA15_08755"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845089.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX9587821.1"
                     /translation="ELGADTTFHRLLYDDAAMLPDEKIKLVPDDKIRRVVLCSGKVYY
                     DLYEEREKRGIDDVYIMRVEQLYPVPLKALVQELGRFKSAELVWCQEEPRNMGAWHFI
                     EPYLEWVLNQINAPNKRPHYAGRAASAATATGLMSKHLAQLKALLDEALS"
     gene            601..1860
                     /gene="odhB"
                     /locus_tag="VGA15_08760"
     CDS             601..1860
                     /gene="odhB"
                     /locus_tag="VGA15_08760"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX9587822.1"
                     /translation="MTEIRVPTLGESVTEATIGRWFKKAGDAVAVDEPLVELETDKVT
                     IEVPAPSAGTLGDIIAKDGETVAVGALLGQINDGAAAAAGKPAAAPAKAAAPPSPPAA
                     AASAAASATTTQRVPAADAPLAPSVRKLSAESGLDAATVPGSGKDGRVTKGDMLAAIE
                     KAASAPTPINQPAAAVQVRAPSPADDAAREERVKMTRLRQTIARRLKEVQNTAAMLTT
                     FNEVDMSHIMAMRSQYKDVFEKKHGIKLGFMGFFTKACVQALKDIPAVNAEIDGTDLI
                     YKNYYHIGVAVGTDKGLVVPVVRDCDLKSIAEIEKSIADFGRRARDGQLKIDEMQGGT
                     FTITNGGIYGSLMSTPILNAPQSAILGMHKIQDRPVAIAGKVEIRPMMYLALSYDHRV
                     IDGKEAVTFLVRVKESLEDPARLVLDL"
     gene            1873..2304
                     /locus_tag="VGA15_08765"
     CDS             1873..2304
                     /locus_tag="VGA15_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9587823.1"
                     /translation="MAFGPNPPPIQAHICVKDGLEAIAFYEKAFGAKCPFHQLAEDGK
                     RVMHANIELFGSEVMLHDEFPEFGSDVLSPSSRGGASMAINVNLPKPTDVDAAVDRAR
                     SAGAEVVMSAQDTFWGARYGRVRDPFGHVWAFNAPLKENAA"
     gene            2345..3091
                     /locus_tag="VGA15_08770"
     CDS             2345..3091
                     /locus_tag="VGA15_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9587824.1"
                     /translation="MTDRVAVITGGSRGIGRATAIAAAARGFKVCVGYASNEAAARSV
                     VSAIEANNGKAIAVKCDVGNETDILALFKAADKFGTLGALINNAGIVGPTSRVDEMSA
                     ERIQRMMAVNVTGSILCAREAVKRMSTRHGGQGGVIVNLSSVASKLGSPNTYVDYAAS
                     KGAIDSFTIGLGHEVAGEGIRVAAIRPGLIDTEIHASGGEPDRAHRLSHMVPMKRVGT
                     ADEIASAIVWLMSDEASYVTSAILDVSGGR"
     gene            3127..>3788
                     /locus_tag="VGA15_08775"
     CDS             3127..>3788
                     /locus_tag="VGA15_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9587825.1"
                     /translation="MATYDLVVIGTGPGGYVCAIRAAQLGMKVAVVEKNATLGGTCLN
                     VGCMPSKALLYASEMFEEAGHSFAKMGVSVSAPKLDLPSMMNFKQQGIDGNVKGVEFL
                     MKKNKIDVISGKGKILGTGKIEVSGSETRIVETKNIVIATGSDIARLKGIEIDEKRVV
                     SSTGVLSLDQVPEKLLIVGAGVIGLELGSVWHRLGAKVTVVEFLDRILPGMDGEVARQ
                     FQR"
BASE COUNT          751 a         1159 c         1213 g          665 t
ORIGIN      
        1 tgagctcggc gccgacacca cgttccatcg cctgctttac gatgatgcgg cgatgctgcc
       61 ggacgaaaag atcaagctgg tgcccgacga caagatccgc cgcgtcgtgc tgtgctcggg
      121 caaggtctat tacgacctct atgaggagcg tgaaaagcgc ggcatcgacg atgtctatat
      181 catgcgcgtc gagcagctct atccggtgcc gctaaaggca ttggtgcagg aactcgggcg
      241 cttcaagagt gccgaactgg tctggtgcca ggaagagccg cgcaacatgg gcgcgtggca
      301 tttcatcgag ccctatcttg agtgggtgct gaaccagatc aacgcgccga acaagcgtcc
      361 gcattacgcc gggcgcgccg cgtccgcggc gaccgccacc ggtctgatgt ccaagcatct
      421 ggcgcagctc aaggcgctgc tcgacgaggc gttgagctga gaaaattgta atccgtcatg
      481 ccccgcgaaa gcggggcatc cagtaacccg ggccgtctac ctcgatcatg atcgccacgg
      541 attactggat cgttcgcttt cacggacgat gatcagacag ggtatctggg gaaccaacca
      601 atgactgaaa ttcgcgttcc gacgctcggt gaatccgtga ccgaggccac catcggccgc
      661 tggttcaaga aggccggcga tgccgtggcg gtcgatgagc ccttggtcga actcgagacc
      721 gacaaggtca ccatcgaagt cccggcgccg tcggcgggca cgctcggcga tatcattgcc
      781 aaggatggcg aaaccgtagc cgtcggcgcg ttgctcggac agatcaatga cggcgcggcg
      841 gcagcggccg gcaagccggc tgctgcgccg gccaaggccg cggcgccgcc gtcaccgcca
      901 gctgccgccg cgtccgccgc cgcatcggcc accacgactc aaagagttcc ggcggctgac
      961 gcgccgttgg cgccgtcggt gcgcaagctc tccgccgaaa gcgggctcga tgccgccacc
     1021 gtccccggct cgggcaagga tggccgcgtc accaagggcg acatgctcgc cgcgatcgaa
     1081 aaggcagcat cggcgccgac gccgatcaat cagcccgccg ccgcggtgca ggtgcgcgcg
     1141 ccgtcgcccg ccgacgatgc cgcgcgcgaa gagcgcgtga agatgacgcg gctgcgccag
     1201 accatcgcgc gccgcctgaa ggaggtgcag aacaccgccg cgatgctcac gaccttcaac
     1261 gaggtcgaca tgagccacat catggcgatg cggtcccagt acaaggacgt gttcgagaag
     1321 aagcacggca ttaaacttgg atttatgggc tttttcacca aggcctgcgt gcaggcgctg
     1381 aaggatattc cagctgttaa cgccgagatc gatggcaccg atctgatcta caagaattat
     1441 tatcacatcg gcgtcgcggt cggcaccgac aagggcctgg tggtgccggt ggtgcgcgac
     1501 tgcgacctca agtcgatcgc cgagatcgaa aagagcatcg ccgatttcgg gcggcgcgcg
     1561 cgcgacggcc agctcaagat cgacgagatg cagggcggca ccttcaccat caccaatggc
     1621 ggcatctatg gttcgctgat gtcgacgccg atcctgaacg caccgcagtc cgccattctg
     1681 ggcatgcaca agattcagga tcgcccggtc gcgatcgccg gcaaggtcga gatccgcccg
     1741 atgatgtatc tggcgctgtc ctacgatcac cgcgtcatcg acggcaagga agcggtgacg
     1801 ttcctggttc gcgtcaagga aagcctcgaa gatccggcgc ggctggtgct ggatctgtaa
     1861 cggagcgcgg ccatggcgtt cggacccaat cccccgccaa tccaggcgca tatttgcgtc
     1921 aaggacggac tcgaggcgat cgctttctac gagaaggcgt tcggcgccaa gtgcccgttt
     1981 catcaactcg ccgaagacgg caagcgggtc atgcacgcaa atattgaatt gtttggcagc
     2041 gaagtaatgc tgcacgacga atttccggag tttggcagcg acgtgttgtc gccatcgagc
     2101 cgtggcggcg ccagcatggc catcaatgtc aatttgccga agccgaccga tgtcgatgcg
     2161 gcagtcgacc gcgctcgatc ggcgggcgcc gaggtcgtga tgagcgcgca agatacgttc
     2221 tggggtgctc gttatggccg cgtgcgcgat ccctttggcc atgtctgggc gttcaacgca
     2281 ccgctaaagg agaatgcggc ttgatcaatc atgcgatcat cgttcgcggc acacgggggc
     2341 aaatttgaca gacagggttg cagtgatcac cggcgggagc cgaggcatcg gccgcgccac
     2401 cgcgattgcc gccgccgcgc gcggcttcaa ggtttgcgtc ggctacgcca gcaatgaagc
     2461 cgccgcccgc agcgtggttt ccgccatcga ggcgaacaac ggcaaggcca tcgcggtgaa
     2521 atgcgatgtc ggcaacgaga ccgatattct cgcgctgttc aaggctgccg acaaattcgg
     2581 aacgctcggc gcgctcatca ataatgcggg catcgttggg ccgacatcgc gggttgacga
     2641 gatgtccgcc gagcgcattc agcgcatgat ggccgtcaac gtcaccggca gcattctgtg
     2701 cgcgcgcgag gcggtgaagc ggatgtcgac gcgccacggc ggccagggcg gcgtcatcgt
     2761 caacctgtcg tcggttgcct cgaaactcgg ctcgcccaat acctatgtcg attacgcggc
     2821 ctccaagggc gccatcgatt cgttcacgat cggccttggc cacgaggtcg ccggcgaggg
     2881 cattcgcgtc gccgcgatcc ggccggggct gatcgatacc gagattcacg ctagcggcgg
     2941 cgagcctgac cgcgcgcatc gattgagcca tatggtgccg atgaagcgcg tcggcaccgc
     3001 cgatgaaatc gccagcgcca tcgtctggct gatgtcggac gaggcctcct acgtgaccag
     3061 cgccatactt gatgtatccg gcgggcgcta atacccgtca cagtttttag ctacaggact
     3121 cccatcatgg ctacctacga tctcgttgtc atcggcaccg gccccggtgg ctacgtctgt
     3181 gcgatccgcg cggcgcaact cggcatgaag gtcgccgtgg tcgagaagaa cgcaacgctg
     3241 ggcggcacct gcctcaatgt cggctgcatg ccgtcaaagg cactgttgta tgcttccgag
     3301 atgttcgagg aggccgggca ttcgtttgcc aagatgggcg tcagtgtctc cgcgccaaaa
     3361 ctcgatctgc cctcgatgat gaatttcaag cagcagggta tcgacggtaa cgtcaagggc
     3421 gtcgagttcc tgatgaagaa aaacaagatc gacgtcatca gcggcaaggg caagatcctc
     3481 ggcaccggca agatcgaggt gtcaggcagc gagacgcgga tcgtcgaaac caaaaacatc
     3541 gtcattgcca ccggctccga tatcgcgcgc ctgaagggca tcgagatcga cgagaagcgc
     3601 gtcgtgtcgt cgaccggcgt gctgtcgctc gaccaggtgc cggagaaatt attgatcgtc
     3661 ggtgccggcg tgatcgggct cgaactcggc tcggtgtggc accggctcgg cgcaaaggtc
     3721 accgtggtgg agtttctcga ccgcatcctg ccgggcatgg atggcgaagt cgcaagacaa
     3781 ttccagcg
//
LOCUS       DASWGM010000302         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1267247,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000302 DASWGM010000000
VERSION     DASWGM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267247"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VGA15_08780"
     CDS             complement(<1..1334)
                     /locus_tag="VGA15_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX9587826.1"
                     /translation="MTSRSPSALHKSTAPRKSAIKTSTAKAKTENKTGKLPEWNLADL
                     YSGIDAPEVTRDLQKMDAECVAFETDYKGKLAEHTAGEGGGQWLAEAIRRYEAIEDLA
                     GRLGSYAGLIHAGDSVDPAISKFYGDVSERLTSASVHLLFLALELNRVDDATMERAMQ
                     TPELGHYRPWIEDLRKDKPYQLEDRVEQLFHEKSQSGYAAWNRLFDQTISGLRFKVSG
                     RELAIEPTLTLLQDRAPEKRKAAGQALAKTFKANERTFALITNTLAKDKEISDRWRGF
                     ADVAASRHLANRVEREVVDALVGSVRSAYPRLSHRYYQLKAGWFKKKKLAHWDRNAPL
                     PFAATGSIAWPDAKNMVLTAYRGFSPEMADIAARFFTDRWIDAPVRPGKAPGAFSHPT
                     TPSAHPYVLMNYQGKPRDVMTLAHELGHGVHQVLAARNGALMAPTPLTLAETA"
     gene            1480..2991
                     /locus_tag="VGA15_08785"
     CDS             1480..2991
                     /locus_tag="VGA15_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9587827.1"
                     /translation="MAASILIADDDAVARRLVENMVQKCGYETLVVDSGDAAIARLTA
                     PDAPVIDAVVLDLVMPGLDGMGVLAKIREAGLSVPVIVQTAHGGIDNVVSAMRAGAQD
                     FVVKPVGIERLQVSLRNALDASALKGELQRIRHSREGRLTFADIITRSETLAGVLRTA
                     QKAAASTIPVLIEGESGVGKELFARAIHGSGERKSKPFVAVNCGAIPDNLVESILFGH
                     EKGAFTGATERHTGKFVDASGGTLFLDEISELPLAAQVKLLRALQEGAVEAVGGRKPV
                     KVDVRIVSATNRKLLERVKSGQFREDLFYRLHVLPLTIPPLRMRREDIPHLLRHFLAR
                     FCAEENRLISGISGEAMTRLSQLEWPGNIRQLENAVYRAVVMSEGDQLGLADFPQVHT
                     QPQAMPDTHNQVEPLVVGPAFHSTVPAMVSGNEIPIAPLPSGGTLAMLTRSGEMRPLE
                     EMETEIIRFAISHYRGQMSEVARRLKIGRSTLYRKLDEAAADESAADGRDETN"
     gene            complement(3143..>3336)
                     /locus_tag="VGA15_08790"
     CDS             complement(3143..>3336)
                     /locus_tag="VGA15_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587828.1"
                     /translation="SNPARLVEQSRIVTPLGETAPSWNSSWIVYRPSQLSPACNDSHA
                     DDTRATDMQPAPRRQLTTN"
BASE COUNT          625 a          991 c         1084 g          636 t
ORIGIN      
        1 gcggtctccg ccaatgtcag cggcgtcggc gccatcagcg cgccgttcct ggccgccagc
       61 acctgatgca cgccgtgacc gagttcatgg gcgagcgtca tcacgtcgcg cggcttgccc
      121 tgatagttca tcagcacgta agggtgcgcc gacggcgtgg tgggatgcga gaacgcgccc
      181 ggcgcctttc cgggccgcac cggcgcatcg atccagcgat cggtgaaaaa tcgcgcggcg
      241 atatcggcca tttcgggcga gaatccgcgg taggccgtca gcaccatgtt cttggcgtca
      301 ggccaggcga tgctgccggt ggcggcaaac ggcagcggcg cattgcgatc ccaatgcgcg
      361 agtttcttct ttttgaacca gcccgccttc aactgataat agcgatgcga cagccgcgga
      421 taggccgatc gaaccgaccc caccagcgcg tcgaccacct cgcgctcgac gcggttggcg
      481 agatgccgcg aagccgccac gtcggcaaag ccgcgccagc ggtcggatat ctccttgtcc
      541 ttggcgagcg tattggtgat gagcgcaaag gtccgctcgt tggccttgaa ggttttcgcc
      601 agcgcctgcc ccgccgcctt gcgcttctcc ggcgcacggt cctgcagcaa agtgagggtc
      661 ggctcgatcg ccagttcgcg ccccgagacc ttgaagcgca ggccggagat ggtctggtcg
      721 aacagccggt tccaggcggc atagccgctc tgggattttt cgtggaacaa ctgctcgaca
      781 cgatcctcga gctgatacgg cttgtccttg cgcagatctt cgatccaggg acgataatgt
      841 ccgagctcgg gggtctgcat tgcgcgttcc attgtcgcat cgtcgacgcg gttgagctcg
      901 agcgcgagaa acaacagatg caccgacgct gacgtcagcc gctcggaaac gtcgccgtag
      961 aatttggaga tcgcaggatc gacgctgtcg ccggcatgga tcaggccggc atacgagccg
     1021 agccgcccgg cgagatcctc gatcgcctca tagcgcctga tcgcttcggc cagccattgg
     1081 ccgccaccct cgcccgcggt gtgttcggcg agcttgcctt tgtagtccgt ctcaaacgcg
     1141 acgcattcgg cgtccatctt ttgcagatcg cgcgtgactt ccggcgcgtc gatcccggaa
     1201 taaagatcgg ccaggttcca ttccggcagc tttccagtct tgttttcagt cttggctttg
     1261 gcggtggagg tcttgatggc tgatttgcgt ggagcggtcg acttgtggag agcggacggg
     1321 gagcgcgagg tcatcttgct tgagcctgtg tatgcgtttc aaccaggaag gtggggacag
     1381 ccgggcgaga acgggacgtc aacaattaag agggcgttaa tcgccatcgg cgagagtgcc
     1441 ccgattcgag acagatagtt gtaaagttgg gggaaagcca tggctgccag cattttgatc
     1501 gccgatgacg acgcggtagc gcgccgtctg gtcgaaaaca tggtgcagaa gtgtggctac
     1561 gagacgctcg tcgtcgattc cggcgacgcg gcgatcgcga ggctgaccgc gccggacgcg
     1621 cctgtgatcg atgccgtcgt gctcgatctg gtgatgcctg gcctcgacgg catgggcgta
     1681 ttggccaaaa tccgtgaagc cggtctcagc gtccccgtca tcgtgcagac cgcccacggc
     1741 ggcatcgaca atgtggtgtc ggcgatgcgc gccggcgctc aggattttgt ggtcaagcca
     1801 gtcggcatcg agcggctgca ggtatcgctg cgcaatgcgc tggacgccag cgcgctgaag
     1861 ggtgagctgc agcgcatccg gcacagccgc gaaggcagac tgacgtttgc cgatatcatc
     1921 acccgcagcg agaccttggc gggcgtgctg cgcacggcgc aaaaggccgc cgcctcgacc
     1981 ataccggtgc tgatcgaagg cgaatccggc gtcggcaagg aattgttcgc ccgcgccatc
     2041 catggcagcg gcgagcgcaa atcaaagccg ttcgtggccg tcaattgcgg cgcgattccc
     2101 gacaatctgg tggagtcgat cctgttcggc catgagaagg gcgccttcac cggcgccacc
     2161 gaacgccaca ccggcaaatt cgtcgacgcc tccggcggca cgctgttcct cgacgaaatc
     2221 agcgaattgc cgctggccgc ccaggtgaaa ctgcttcgcg cgctgcagga aggcgcggtg
     2281 gaggcggtcg gcgggcgcaa gccggtcaag gtcgatgtcc gcatcgtctc ggcgaccaac
     2341 cgcaagttgc tggagcgggt caagagcgga caattccgcg aagatttgtt ctaccgcctc
     2401 cacgtgctgc cgctgacgat tcctcccttg cggatgcggc gcgaagacat cccgcatctg
     2461 ttgcggcatt ttctggcgag gttctgcgcc gaagaaaacc gtctcattag cggcatcagc
     2521 ggcgaggcga tgacccgtct gtcgcaactc gagtggcccg gcaacatccg ccagcttgag
     2581 aacgcggtct atcgcgccgt tgtcatgagc gagggcgacc aactcggcct tgcggatttt
     2641 ccgcaagtcc acacgcaacc gcaggcaatg ccggacaccc acaaccaggt cgagccgctg
     2701 gtcgtcggtc cggcgtttca ctcgaccgtg cccgccatgg tttcaggtaa cgagatacct
     2761 attgcgccgt tgccgtcagg cgggacgctg gcgatgctga cgagatcagg agaaatgcgg
     2821 ccgcttgaag agatggagac cgagatcatc cggttcgcga tctcgcatta tcgcgggcag
     2881 atgtccgagg tcgcacgccg attgaagatc ggccgctcga cgctttaccg caagctcgat
     2941 gaggctgccg ccgacgagtc ggcggccgac ggaagagacg agacaaactg agacaatgtc
     3001 ggattgtgac actccggtgt ttgaaatgaa gcagaagaca ttggcgattc cagaacattt
     3061 gaacagttgc ttgctggtga cagacaaagc gaagggcgca tggcaaaagc gcgtaatcgg
     3121 ttgaaaccgg aaccgcttga agtcagtttg tcgtgagttg cctgcgtggc gctggctgca
     3181 tgtcggtggc gcgtgtatcg tctgcatgtg aatcgttgca tgccggcgaa agctgtgacg
     3241 gacgatagac gatccaagag ctgttccatg acggagcagt ttcgccaagg ggtgtgacga
     3301 tgcgtgactg ttcgaccaac cgtgctggat ttgacc
//
LOCUS       DASWGM010000303         1926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1828464,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000303 DASWGM010000000
VERSION     DASWGM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828464"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(561..>1926)
                     /locus_tag="VGA15_08795"
     CDS             complement(561..>1926)
                     /locus_tag="VGA15_08795"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HEX9587829.1"
                     /translation="RASEIVTRELGRQSELEVTKQLEREVTADRFTGLDRMLIAEQQG
                     KEFSDLRPDQDMRDTFRQNRALLIERARKLERMGLATEVETGIWMVSPKAEPVLRELG
                     ERGDIIKTMQRALEREGLAEDRHPARYILHRENATERIVGRVLDKGLGGDEMGERVRL
                     VIDGLDGRVHHIEMDAARAEDVGRGMIVVAGSAPPGPRAADRNILDIAGQGGVYRPSQ
                     HLERARTAIDRIGGDPEAFVRSHVRRLEALRRAGHAERIDADHWRVPTDLSARGQAYD
                     LARDRANIRISILSPTGLDSQIRHDGATWLDREMASPSRTTLANTGFGREVTEAMERR
                     RQSLVNMGHAVRLEDGRIRVSKDLIANLERTEVTRVGKAMAAERGLTFTAAKTGEYVS
                     GALVGSTQLASGRFAMIDDGIGFQLVPWQPLLEKRIGQHITGTVRDAGGIEWSFSRKR
                     GLGI"
BASE COUNT          333 a          647 c          584 g          362 t
ORIGIN      
        1 gagcgtcacg tcccaaactc gatcgccacc gtgcgccgac gcatcatcga cgctctcgtc
       61 aggcgcttgc cacgatgccc atgctgtcgc gcttcaaccg cgcgacgcgt gcggatgaac
      121 ttgtgacgca gtagtactag ctcggcatgc ttcggctcat cggccaacgc accataatca
      181 tttttggcac catttccgag ctgcggatca cgtcgcacca acgttacgcc cccttccggg
      241 acttcagtcg aagccgtaac cctgaggcgt gtcgcggatc acgtcaatgg aagcggcaga
      301 gtagaacggc gcacctcagc gaatatcagg aaagactgga atagctccgg cgccgaaagg
      361 cggcgctgga tttttccaac tggcgtcttc tgaatattca catcgccctg gccttctccg
      421 gacacgttcg ttgctggacc cgcaaccacc gatccttgcg attggttgaa aaagttggtt
      481 aagcgccgaa aagtgacgtc tttgccggat ggcgctggtc tggccccatg gcgataagca
      541 ggtcacgatg ttagtgaccc tcatatcccc agcccccgtt tcctgctgaa actccattcg
      601 atgccgccag catcgcgcac tgtgccggtg atatgctggc cgatgcgctt ttcaagaagg
      661 ggttgccacg gcaccaactg gaatccgatg ccgtcatcga tcatggcgaa gcggccgctc
      721 gcgagctggg tggagccgac aagcgcgccg ctgacgtatt ccccggtctt ggcggcggtg
      781 aacgttagac cgcgttcggc agccatcgcc ttgccgacac gagtcacctc cgtacgctcc
      841 agattggcaa taagatcctt tgacacacgg atacgtccgt cctccagccg gacggcgtgg
      901 cccatgttca caaggctctg cctgcggcgt tccattgcct cagtgacctc ccggccgaac
      961 ccggtattgg caagcgtggt acggctgggc gaggccattt cccggtcgag ccaagtcgcg
     1021 ccgtcatggc gaatctggct gtcgaggccg gtaggagaca ggatgctgat gcggatgtta
     1081 gcgcggtccc gcgccagatc gtaggcttga ccgcgcgcgg agagatcggt ggggacgcgc
     1141 cagtgatcgg cgtcgatgcg ctcggcatgg cccgcccggc gcagcgcctc cagccggcgg
     1201 acgtgggagc gaacgaaggc ctcgggatcg ccgccgatac ggtcgatggc agttcgcgcc
     1261 cgctccagat gctgggacgg gcgatagacg cctccctgac ctgcgatatc caggatgttg
     1321 cggtccgcgg cgcgcggacc gggaggggcg gagccggcca cgacgatcat gcctcggccg
     1381 acgtcctcag cgcgggctgc atccatctcg atgtgatgca cgcgcccatc caagccgtcg
     1441 atcaccagcc gaacccgctc gcccatctcg tcgccgccaa gccctttgtc gagcacgcgg
     1501 ccgacgatgc gctccgtcgc gttctctcgg tgcaggatgt agcgcgcggg atggcggtcc
     1561 tccgccagcc cttcgcgctc cagtgctcgt tgcatggtct tgatgatgtc gccgcgctcc
     1621 ccaagctccc gcagcaccgg ctcggccttt ggcgaaacca tccaaatgcc ggtctcgacc
     1681 tcagtcgcca gccccatacg ctccagcttg cgcgcgcgct cgatcagcag ggcgcgattc
     1741 tgacgaaagg tgtcgcgcat gtcctggtcg ggacgcagat cggaaaactc cttcccctgc
     1801 tgctcggcga tcagcatccg gtcgagcccg gtgaagcgat cggcagtcac ctcccgctca
     1861 agctgcttcg tgacctcgag ctcgctctgc cggcccagtt cccgggtaac aatctcgctg
     1921 gcccgc
//
LOCUS       DASWGM010000304         9510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1828489,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000304 DASWGM010000000
VERSION     DASWGM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828489"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VGA15_08800"
     CDS             <1..804
                     /locus_tag="VGA15_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX9587830.1"
                     /translation="AYLAAGPVNSKITADAIAASTRDGGTPTFLAIDSAEAIAQNHPM
                     YESAEIPAGAFGGAPGRPDEEVKTISFAHHIVARKGLSEATVAAFTRQLFAIRQAVMA
                     EFPLAAKIETPDTDKDAVIPVHPGAAAYVDGEEKTFLDRYSDFIWWGLMGLSAMGSAG
                     AWFAGYLKKDVRSNNSSLRERLLDMLGAARRSDSTEELDQMQTEADDILRDTLQCFEH
                     GAIEEGALTAFNIALEQFHNAVADRKALLMSLPQNLQRANVALRASGTL"
     gene            complement(808..2259)
                     /locus_tag="VGA15_08805"
     CDS             complement(808..2259)
                     /locus_tag="VGA15_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX9587831.1"
                     /translation="MARRAVIRRLRRWLYIGHRWIGIATCLLFAMWFVSGLVMMYVAF
                     PTLTDGERLAALPDIAWDRVRLTPDRAMAAAGLTHYPRDLRLNMLNDEPVYRLLDGDG
                     RRQTVSAVDGRVIDSVSLEQALAVARRYPAAASVQLAGSVDRDQWSVTARFDSVRPFF
                     LVSLGDADGTELYVSSRSGEIVLDTTRTERIWNWLGSIPHWIYPTVLRKGGALWRQVV
                     LWISGICMLVAASGIWIGILRVRLRRRYSSGGITPYRGWMAWHHVTGFFAGVFVLTWM
                     FSGWLSLDPGEFFARRGPSREMVLRYAGHDAPTMAGEFLSRPPIAAVEARFAWLGGKP
                     LMLLADRSGQQTSADPATGMAAPLSDAMIFEAARRLMPQAAMTMQRRLDRYDAYWYAH
                     HQPRALPMLRAGFDDAAQTWFHIDPRSGEILGRTDSSGRSYRWLFNALHSFDFPWLLA
                     WRPAWDIVVWLLSLLGLTIAVSGIVIGCRRLVR"
     gene            complement(2259..4514)
                     /locus_tag="VGA15_08810"
     CDS             complement(2259..4514)
                     /locus_tag="VGA15_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX9587832.1"
                     /translation="MTSVPLRASRRTSLLCCFALCTFWPVDAWTQGAPVSRDLDPVVV
                     TSPAPPAATKRRGGDKRAASSARSRRQRAAVATAAAPAPSPAASAAPTLNLTGPASSG
                     SRLNLTRLQTPASVEVITAETIAERGQHNVLDAVTQNATGFTASPAPGNGSLAFNTRG
                     FSGNGTVMTLYDGTRLYVGAGTLTFPFDTWSAQRIEVLRGPASVMYGEGAIGGAINVI
                     SKMPLTTPRNEGEISLDTNLTRRLAVDSGGPINKDVSYRVTAIGNMSDGWVDRDKTSN
                     VAVSAAVRVQASDTLAWTLSTDYGDRGPLRYFGTPLVNGRLDESLRFKNYNVNDSNIR
                     YRDSWNQLKTEWDVTDAISIHNVVYYLNSQRHWKDVESYAWNPVTGLIDRKSYIEIFH
                     NQQQTGDRMDATFHGHVLGMMNEFVAGFDVNRIDFAHTNNSPFSGASSVNPVNFDPGL
                     FLSPIATVPSFSSVTNQYALFAEDRLSVTDQFSLVGGVRQDQPAIARTDLITPANGFE
                     KSFSATSWRAGAVYTPVKDLAFYGQFSTAVDPVGNLITLTLPNKDFQLASGKQAEIGV
                     KQSFWGGRGEWTLAGYRIVKNNLLTPDPNDPTKVQQVGQQSSRGIEATAGLALAGGWR
                     IDANTAWLRAKYDDFVQSRTVNFAGNIPVNVPQNVSNIWLTFAFAPDWSVNGGVQMVG
                     NTFADNANTLTRPSYTLVNAGLQWRPDAKTTMALRVYNLFDTIYATGGPTTQWLLGMP
                     RTAELSLNVKF"
     regulatory      complement(4622..4830)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4998..6548
                     /locus_tag="VGA15_08815"
     CDS             4998..6548
                     /locus_tag="VGA15_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX9587833.1"
                     /translation="MAIRLSRRRFLSTTAATGFATLAMPYLSRAADRPQITHGVQSGD
                     VGADGGVVWARADRPSQMMVEVATTESFGNARALPPIAALPESDFTAKMLLENLPAGQ
                     DIFYRVKFRDLSHTDVSSEPVVGRFRTAPADRRDVSFVWGGDVAGQGWGINPDDGGML
                     TYATMRKHKPDFLLHSGDTIYADGIIPAEVKLPDGKVWKNVTIPEKAKVAETLDEFRA
                     AHKYNFLDDNVRAFNSEVPIFVQWDDHEVTNNWSASKQLPAAYKERDIALLAARAGRA
                     FHEMYPMRESIVEPGRVYRTLSYGPHLDLFMLDERSYRGPNGPNLQTAYGPDSYFIGP
                     DQLLWLKRALLNSRATWKVIASDMPLSLVVYDDATNKKGSEAFAQGDGPPRGRELEIA
                     EILRFIKTSGVTNTVWLTADVHYTAAHYYNPDKAQFQDFDPFWEFVSGPLHAGTFGPN
                     ELDNTFGPEVKFIKAPGLDKQNLPPSAGMQFFGHVKIEGATGQMTVTLRDRADVALWS
                     TTLDPKLG"
     gene            complement(6597..6995)
                     /locus_tag="VGA15_08820"
     CDS             complement(6597..6995)
                     /locus_tag="VGA15_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9587834.1"
                     /translation="MKPASADGTAALPNDVLIVEDDPLIALDFEDTILGFGVKTVRTA
                     GNVARALEMIADRAPDFALLDVGLVREKSFAIAERLDALKIPFVFVTGYGAAGTFPAA
                     FARRPRLPKPCSAEELKALLYRQSEGAAKG"
     gene            7164..7934
                     /locus_tag="VGA15_08825"
     CDS             7164..7934
                     /locus_tag="VGA15_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9587835.1"
                     /translation="MATQRGATPEGIERPFNNLLRRLSASDFALIGPHLAHEEAAAND
                     LLYNPGDDVEIVHFPCGPSLATYLVSNEDGRDVETILVGREGAVGGIVSQGYLPAYTR
                     IMVRFGGPFVRLHVGKLDAAKTKSATLRNVFARYADCMLAQIFQSTACNAIHSIEQRT
                     AKWIISAMERTDDADTVPLTHEQLATMLGVGRSYVSRVIQTFKAEGVLETRRGSILVR
                     DREALRVRACLCNESVKSHFEEVLRGVYPSDEARGASK"
     gene            8295..>9510
                     /locus_tag="VGA15_08830"
     CDS             8295..>9510
                     /locus_tag="VGA15_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9587836.1"
                     /translation="MKLRNVAIIAHVDHGKTTLVDRLLQQSGTYRDNQRVVERAMDSN
                     DLERERGITILAKAASVQWKDTRINIVDTPGHADFGGEVERILNMVDGALVLVDAAEG
                     PLPQTKFVVSKALKVGLKPIVVINKVDRPDARPTEVINEVFDLFAALDASEEQLDFPI
                     LYGSAKQGWMAESPDGPKDAGMQPLFDLILRHVAPPTVEDGPFRMIGTILEANPYLGR
                     IITGRITSGTIKPNQAVKVLGGDGKLIETGRITKIIAFRGIERTPLDEAEAGDIVAIA
                     GLTKGTVADTFCDPSVDTPLRAQPIDPPTVSMSFIVNNSPLAGTEGDKVTSRMIRDRL
                     LREAEGNVALRVTEAADKDSMEVSGRGELQLAILIETMRREGFELSVSRPRVVLKKDE
                     ATGEWQEPVEEVV"
BASE COUNT         1856 a         3031 c         2972 g         1651 t
ORIGIN      
        1 gcctatctcg ccgccggacc ggtcaacagc aagattaccg ccgatgcgat cgcggcatcg
       61 acacgcgacg gcggcacgcc gacctttctc gcgatcgatt cggccgaggc gatcgcgcaa
      121 aaccacccga tgtacgaatc cgccgaaatc cccgccgggg cgttcggcgg cgcgcccggc
      181 aggccggacg aagaggtgaa gacgatcagc ttcgcccatc atatcgttgc acggaaaggt
      241 ctgtcggaag cgaccgtcgc cgcctttacc cggcagttgt ttgcgatcag gcaggcggtg
      301 atggcggaat tcccgctggc ggcgaagatc gagacacccg acaccgacaa ggacgccgtg
      361 atccccgtgc atccgggtgc ggcggcctac gtcgacggtg aggaaaaaac cttcctggac
      421 cgctacagcg atttcatctg gtgggggctg atgggtctgt ctgcgatggg ctcggccggc
      481 gcgtggttcg cgggctatct gaaaaaggat gtgcgcagca acaacagttc attgcgcgaa
      541 cggttgctgg acatgcttgg cgccgcgcgc cgcagcgatt ccaccgaaga gctcgaccag
      601 atgcagaccg aggcggacga tatccttcgc gatacgctgc agtgcttcga gcacggcgcc
      661 atcgaggaag gggcgttgac cgctttcaat atcgcgctcg agcaattcca caacgccgtg
      721 gccgaccgca aggcattgct gatgagcctg ccgcagaacc tgcaaagagc caacgtcgcg
      781 ctgcgcgcca gcggcacgct gtagaaatca gcgcaccagc cggcggcagc cgatcacgat
      841 cccgctcacc gcgatcgtca accccagcag cgacagcagc cacaccacga tatcccacgc
      901 cggacgccag gccagcagcc atgggaaatc gaagctgtgc agcgcgttga acagccagcg
      961 ataggagcgc ccgctggaat cggtgcggcc caaaatctcg ccgctgcgcg gatcgatgtg
     1021 aaaccaggtt tgcgcggcat cgtcgaagcc cgcgcgcagc atcggcagcg cgcgcggctg
     1081 gtggtgcgca taccaataag catcgtagcg atcgagacgc cgctgcatcg tcatcgcggc
     1141 ctgcggcatc agccggcgcg cggcctcgaa aatcatggcg tcagacaacg gcgccgccat
     1201 gcccgtcgcc ggatcggcgg acgtttgttg gccgctccga tccgccagca gcatcaacgg
     1261 cttgccgcca agccaggcga agcgcgcttc caccgctgcg atcggtgggc gcgaaaggaa
     1321 ttccccagcc atggtgggcg cgtcatggcc ggcataacgc agcaccatct cgcgcgacgg
     1381 tccacggcgg gcgaagaatt cgccgggatc gagcgacagc cagccgctga acatccaggt
     1441 cagcacgaac acgcctgcaa agaatcccgt gacatgatgc cacgccatcc agccgcgata
     1501 gggcgtgatc ccgccgctgg aatagcgccg ccgcagccgc acgcgcaaaa tcccgatcca
     1561 gatgccgctg gccgccacca gcatgcagat gcccgagatc cacaacacga cctgccgcca
     1621 cagcgcaccg cccttgcgca gcaccgtcgg atagatccag tgcggtatgg atccgagcca
     1681 gttccagatg cgctcggtcc gcgtggtatc gagcacgatc tcaccgctgc gggaggaaac
     1741 gtagagctcc gtgccatcgg cgtcgccaag cgagacaaga aagaacggcc gcacggaatc
     1801 gaatcgcgcc gtgacgctcc attggtcgcg atcaacgctg ccggccaatt gcacgctcgc
     1861 cgcggcagga tatcgccgcg ccaccgccag cgcctgttcg agcgagacgc tgtcgatgac
     1921 gcggccatca actgccgaga ccgtttgccg ccggccatcg ccgtcgagca accgatagac
     1981 cggctcatcg ttcagcatgt tcagccgcag atcgcgcgga taatgcgtga gcccggcggc
     2041 cgccatcgcg cgatccggcg tgagccgtac cctgtcccag gcgatatcgg ggagcgccgc
     2101 aaggcgctcc ccgtcggtga gggttggaaa cgccacatac atcatgacga gaccggacac
     2161 gaaccacatc gcgaacaata ggcacgtcgc aatgccgatc catcggtggc cgatatacag
     2221 ccaccgccgc aaccgccgga tcaccgcgcg gcgcgccatc agaatttcac attgagcgac
     2281 aattccgcgg tgcgcggcat gccgagcagc cattgcgtgg tcgggccgcc agtcgcataa
     2341 atcgtgtcaa acagattgta gacccgcagc gccatcgtcg tcttggcatc cggcctccac
     2401 tgcaagccgg cattgacgag ggtatagctc gggcgcgtca gcgtgttggc gttatcggcg
     2461 aatgtatttc cgaccatctg cacgccgcca ttgacggacc agtccggcgc aaatgcaaag
     2521 gtcagccaaa tgttggagac gttctggggg acgttgacgg gaatgttgcc ggcgaagttg
     2581 acggtcctgg actggacaaa atcgtcgtac ttagcccgca accacgcggt gttggcgtcg
     2641 atacgccagc cgccagccag cgcaagacct gccgtggctt cgataccgcg cgacgattgc
     2701 tggccaactt gctggacctt ggtgggatca ttgggatcgg gagtcaggag gttgttcttg
     2761 acgattcggt agccggccag cgtccactcg ccgcggccgc cccagaacga ttgcttgacg
     2821 ccgatctcgg cctgcttgcc gctggcaagc tggaaatcct tgttcggcaa agtcagcgtg
     2881 atcaaattgc cgactggatc gaccgcggtg gaaaattgtc cgtagaacgc gaggtctttc
     2941 accggggtgt agaccgcgcc ggcgcgccag ctcgttgccg agaacgattt ctcgaaaccg
     3001 ttcgccggcg tgatcaggtc ggtgcgagcg atggccggct gatcctgccg cacgccgccg
     3061 accagagaga actgatcggt gaccgacagc cgatcctcgg caaacagcgc gtactgattg
     3121 gtcacgctgc tgaatgacgg aacggtggcg atggggctga gaaacaggcc aggatcgaaa
     3181 ttgaccggat tgaccgacga cgctccgcta aagggcgaat tgttggtgtg ggcaaaatcg
     3241 atccgattga cgtcgaagcc tgcgacgaat tcgttcatca tgccgagaac atgaccgtga
     3301 aaggtcgcgt ccatccgatc gccggtctgc tgttgattgt gaaagatctc gatataggat
     3361 ttgcggtcga tcaggccggt gacgggattc caggcgtagc tctcgacgtc cttccagtgc
     3421 cgctggctgt tcaggtaata caccacgttg tggatcgaga tggcgtcggt gacatcccac
     3481 tcggtcttga gctgattcca gctgtcgcga tagcggatgt tgctgtcatt gacgttgtag
     3541 ttcttgaacc gcagcgattc gtcgagacgg ccattgacca gcggcgtgcc gaaatagcgc
     3601 aatgggccgc ggtcgccgta atcggtggac agcgtccatg ccagggtgtc gctggcctgc
     3661 acgcgcaccg cggcggacac cgcgacgttc gacgtcttgt cgcgatcgac ccagccatcc
     3721 gacatgttac cgatcgcggt gacccggtag gagacatcct tgttgatcgg gccgccgctg
     3781 tcgaccgcga gccgtctcgt caaattggta tcgagcgaaa tttcgccctc gttgcgtggc
     3841 gtggtcaacg gcattttgga gatcacgttg atggcgccgc cgatcgcgcc ttcgccatac
     3901 atcaccgacg ccggcccacg cagcacttcg atgcgttgcg ccgaccaggt gtcgaacgga
     3961 aacgtcagcg tgccggcgcc gacatagagc cgcgtgccgt catacagcgt catcacggtg
     4021 ccgttgccgg agaaaccgcg ggtattgaac gcgaggctgc cattgccggg cgcgggcgac
     4081 gcggtgaagc cggtcgcgtt ctgggtgacg gcgtcgagca cattgtgctg gccgcgttcg
     4141 gcgatggtct ccgccgtgat cacctcgaca ctggctggcg tttgcagccg cgtgaggttg
     4201 agccggctgc cgctcgatgc tggtccggtg agattgagcg tcggagcggc cgacgctgcc
     4261 ggggacggcg caggcgccgc agccgtggcg acggcggcgc gttgccgccg cgaccgtgcc
     4321 gacgatgcgg cccgcttgtc gccgccgcgg cgcttggtgg ccgctggcgg cgccgggctg
     4381 gtcaccacga ccggatcgag atcgcggctc acgggcgcgc cttgtgtcca ggcatcgacc
     4441 ggccagaagg tgcagagcgc aaagcagcac agcaaagacg tgcgtcggga cgcacgaagc
     4501 gggacggagg tcatcgaatg gactcgcggt taacgtggca cgtcaccgcg aacgaagtct
     4561 ccccacagcc cgattcggcc gaggttcggc taccaccagg acaccccgcc caatgtgctc
     4621 gcgtgtgacc acgactggcg gcaggtctcc tggctcgcgg gtcgctgcct ttcaccgcct
     4681 tcccagacag atatgtccag tggctaatgg cgaaaggctc gccgcttaca gttgcggggg
     4741 cagccgcgga atcgggacca tgttcgaaga actggccctc accgcattcc cttttgatcc
     4801 ccggggggaa ccgtcaccat gacgttatgt tgaaatcgca aaagctgtca acgacgctgg
     4861 gtttgataac gaaacctgga tcattcggac gggcgttcga tcaatccaga ttccaacgtc
     4921 aaacctgtca tcgccgcgtc aaccaacttc cgtaagtgga ggcgatttct ttcgagatct
     4981 tcgatcagga gctcgctatg gccattcgac tgtcccgccg ccgttttctc tcgaccaccg
     5041 cagctaccgg cttcgccacg ctggcgatgc cctacctcag ccgcgccgcc gaccggccgc
     5101 aaattaccca tggcgtgcaa tccggtgacg tcggcgcaga cggcggggtg gtgtgggcgc
     5161 gcgcggaccg gccgtcgcaa atgatggtcg aggtcgccac aacggaatcg tttggcaatg
     5221 cgagggcttt gccgccgatc gcggcgcttc cagagagcga tttcaccgcc aaaatgctgc
     5281 tggaaaatct cccggccggc caggacattt tttatcgggt caaattccgc gacctgtcgc
     5341 ataccgacgt ttcgagcgaa ccggtggtcg gccgcttccg caccgcgccg gccgaccggc
     5401 gcgatgtcag tttcgtctgg ggcggcgatg tcgccggaca aggctggggc atcaatcccg
     5461 acgacggcgg catgttgacc tacgccacca tgcgcaagca caaaccggat ttcctgctcc
     5521 attccggcga caccatctat gccgacggca tcattcccgc cgaggtgaaa ttacccgacg
     5581 gcaaggtctg gaagaacgtc acgatcccgg aaaaggccaa ggtggcggag acgctcgacg
     5641 aattccgcgc cgcgcataag tacaatttcc tcgacgacaa tgtgcgtgcc ttcaactccg
     5701 aggtcccgat cttcgtgcag tgggacgatc acgaagtcac caacaactgg tcggcgtcga
     5761 aacagcttcc cgccgcctac aaggagcgcg acattgcctt gcttgccgcg cgcgccggcc
     5821 gcgcgttcca cgagatgtat ccgatgcgcg aaagcatcgt cgagccgggc cgggtctatc
     5881 gcactctgag ctatggtccg catctcgacc tgttcatgct ggacgagcgc agctatcgcg
     5941 gtccgaacgg cccgaatctg caaaccgcct acggtcccga cagctacttt atcggcccgg
     6001 atcagctgtt gtggctgaag cgagcattgc tgaattcacg cgccacctgg aaggtgatcg
     6061 catccgacat gccgctcagc ctcgtcgtct atgacgacgc gaccaacaaa aagggttcgg
     6121 aagcattcgc gcagggcgac gggccgccgc gcggccgcga gctcgaaatc gccgagatcc
     6181 tgcgcttcat caagacgtcg ggcgtcacca acacggtatg gctgacggcg gacgtgcact
     6241 acaccgctgc gcactactac aatcccgaca aggcgcaatt tcaggacttc gatccgttct
     6301 gggaattcgt ctccggacca ttgcatgccg gaactttcgg gccgaacgaa cttgacaaca
     6361 ccttcgggcc cgaggtgaag ttcatcaagg cgccgggcct cgacaaacag aacctgccgc
     6421 catcggccgg catgcagttc ttcggtcacg tcaagatcga gggtgcaacc gggcagatga
     6481 cggtgacctt gcgggatcgc gccgatgtgg cgctgtggtc gacaacgctc gatcccaaac
     6541 tcgggtagcc aagcggccgt tgatttcgct cgcagcgcgc gaccaaacgt cgccggtcag
     6601 cccttcgctg cgccttctga ctggcgatac aacaacgcct tcagctcttc ggccgaacag
     6661 ggtttcggca gccttggcct gcgcgcgaac gccgccggaa acgtgccggc agcgccgtac
     6721 ccggtgacga acacaaaagg aattttcagc gcatccagcc gctcggcaat ggcaaaactc
     6781 ttttcccgga ccagcccgac atcgagcagg gcaaaatcgg gggcgcggtc ggcaatcatt
     6841 tcgagcgctc tggccacgtt gcccgcggtc cgcaccgtct tcaccccgaa tccaagaatc
     6901 gtgtcctcga agtcgagcgc gatgagcgga tcatcctcga caatcaacac gtcattgggc
     6961 aaggcggccg tgccgtcggc agaggcgggc ttcatgatct cgtcgagcaa gaggaaagga
     7021 cccaggcctg acgcctatgc ggcgcaccgc ccggtttccg cgccggaacc caccacacct
     7081 cgcttcccct gtctgtgcac ttgtgcacac atggggttgg tgaagatggt attggtgggg
     7141 ccagggagga gtgaggttcg ggaatggcca cgcagcgcgg agcaacacct gagggcattg
     7201 aacggccctt caacaacctg ttgcggcggc tcagcgccag cgactttgcg ctgattgggc
     7261 cgcatctcgc gcacgaagag gctgcggcaa acgacctgct ctataatccc ggcgacgacg
     7321 tcgagatcgt ccactttccg tgcggaccga gtcttgcgac atatctcgtc tccaacgaag
     7381 acggccgcga cgtcgaaacc attctggtcg gccgcgaagg cgcggtcggc gggattgtca
     7441 gccagggata tctgccggcc tatacccgta tcatggtcag gttcggcggg ccgttcgtac
     7501 gcctgcatgt cggaaagctg gatgcggcga agacaaagtc ggccaccttg cgcaacgtgt
     7561 tcgcgcgcta tgccgactgc atgctggccc agatcttcca gtcgacggcc tgcaacgcga
     7621 tccattcgat cgagcagcgc acggcgaaat ggatcatctc ggcaatggag cgtaccgatg
     7681 acgccgacac cgtgccgctg acccatgagc aacttgccac catgctgggg gttggccgca
     7741 gctatgtcag ccgcgtgatc cagacgttca aggcggaagg ggtcttggag acccgccgcg
     7801 gctcgatcct ggtgcgcgac cgcgaggcgc tgcgggtccg ggcctgcctc tgcaacgaat
     7861 cggtcaagag ccattttgag gaagtgctgc gcggggtata tccgagcgat gaggcccgtg
     7921 gcgcttccaa gtaatggcgc cggcgccgcc tgcctggcca gttcacgcgg cgcaatagcg
     7981 ctgtcctgat caggctcgcg ctccgcggac agcgacgtta actgtttttg cgcagaagcg
     8041 ccgggggcaa cacctgaagt gccggaaatg cctatgaatt ttgcctgatg cgataaggtt
     8101 acccgttgtt tttcggcgat ttatgactat attgcgccgc aacgagtaaa ggtatgcccg
     8161 gtcctttggc cgggcttcct ttttgggcgt catgcgccct tcgaattcca ggttttgagc
     8221 gtgatccgga aaagtgggtc ccggttttcc ggcgagatca tgctccagcc attgcctgac
     8281 ccagaagaag agctatgaaa cttcgcaacg tcgccattat cgcccacgtc gaccacggca
     8341 agaccacgct ggtcgatcgc ctgctgcagc aatccggcac ctaccgcgac aatcagcgcg
     8401 tggttgagcg cgcgatggat tccaacgacc tggagcgcga gcgcggcatc accattctcg
     8461 ccaaggccgc ctcggtgcag tggaaggaca cccgcatcaa catcgtcgat accccgggcc
     8521 acgccgattt cggcggcgag gtcgagcgca tcctcaacat ggtggacggc gcgctggtgc
     8581 tggtggacgc cgccgaaggc ccgctgccgc aaaccaaatt cgtggtgtcg aaggcgctga
     8641 aggtcgggtt gaagccgatc gtcgtcatca acaaggtcga ccgccccgac gcgcggccga
     8701 ccgaagtcat caatgaagtg ttcgatctgt tcgcagcact cgacgcgtcc gaggagcagc
     8761 tcgactttcc gattctgtac ggatcggcca agcagggctg gatggcggaa agccctgatg
     8821 gtccgaagga cgccggcatg cagccgctgt tcgacctgat cctgcgccat gtcgcgccgc
     8881 cgacggtcga ggacggcccg ttccgcatga tcggcaccat tctggaggcc aatccctatc
     8941 tcggccgcat catcaccggc cgcatcacct ccggcaccat caagcccaac caggccgtca
     9001 aggtgctagg cggcgacggc aagctgatcg agaccgggcg gatcacaaaa atcatcgcgt
     9061 tccgcggcat cgagcgcacg ccactcgatg aggccgaagc tggcgacatc gtcgccattg
     9121 ccggtctcac caagggcacc gtcgccgata ccttctgcga tccttcggtc gacaccccgc
     9181 tgcgggcgca gccgatcgat ccgccgacgg tgtcgatgtc gttcatcgtc aacaactcgc
     9241 cgctggccgg caccgaaggc gacaaagtca ccagccgcat gatccgcgac cgcctgctgc
     9301 gcgaggccga aggcaatgtc gcgctgcggg tgaccgaagc cgccgacaag gattccatgg
     9361 aagtctcggg ccgcggcgaa ttgcagctcg cgatcctgat cgagaccatg cgccgcgaag
     9421 gctttgagct gtcggtgtcg cgcccgcgcg tcgtcctgaa gaaggacgag gcgaccggcg
     9481 agtggcagga gccggtcgaa gaggtcgtga
//
LOCUS       DASWGM010000305         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2524545,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000305 DASWGM010000000
VERSION     DASWGM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524545"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /gene="rsfS"
                     /locus_tag="VGA15_08835"
     CDS             <1..202
                     /gene="rsfS"
                     /locus_tag="VGA15_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX9587837.1"
                     /translation="GAIAENVAKGLKETGIKSIHIEGLPNCDWVLIDSGDVIVHVFRP
                     EVREFYNLERLWTQSPTAAKVI"
     gene            432..914
                     /gene="rlmH"
                     /locus_tag="VGA15_08840"
     CDS             432..914
                     /gene="rlmH"
                     /locus_tag="VGA15_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX9587838.1"
                     /translation="MRLVVISIGRLKQGPERELAERYRERFDDIGRKLGFRGLEIQEI
                     PESRARDAATRMTDEATAISAAIPAKSVLVALDERGDSIDSAAFARHLGRWRDEQVAN
                     TIFVIGGADGLSPDLRRKAKLSIAFGSATWPHQMVRVMLLEQVYRAATILAGHPYHRV
                     "
     gene            1068..>2236
                     /locus_tag="VGA15_08845"
     CDS             1068..>2236
                     /locus_tag="VGA15_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX9587839.1"
                     /translation="MSLLPAAAQVLPAAAQVLPAAAQVLPAAQGAASTQQTTASSPDA
                     IKQREQELEAARAQQKSAAELQMKLQTDLAAIGQDHSKLNQRLIDIAGQVRSVEARIG
                     DAETRLRPLDSREQQIRSSLGSRRSEIVEVLATLQRAGRRMPPALLVRPEDALQSLRT
                     AMLLGSVVPELRGRADRLATDLGELVSVRKNIATERDQLAVDRDKLRDDQTRLAALVD
                     ERQRKQSSIEKDMAAESARAITLSQQVDGLEGLIAKMEQDLKSAARAAASASLRGAPV
                     APNGKPNLGGLKDSARLSPAIAFASAKGLFAFPVNGVKIREFGGSDGSGGVEKGISLS
                     TRAGAQVTTPCDGWVVYAGPFRSYGQLLILNAGGGYHVLIAGMERISVNIGQFVLT"
BASE COUNT          432 a          670 c          716 g          418 t
ORIGIN      
        1 cggcgcgatc gcggaaaatg tcgccaaagg tctcaaggaa accgggatca aaagtatcca
       61 catcgagggc ttacccaact gcgactgggt gctgatcgat tccggggacg tgatcgtgca
      121 cgtattcagg cccgaggtgc gcgagttcta caatctggag cgattgtgga cccagagccc
      181 gacggcggcc aaggttatct aagctcaggg ctatctgagc tgacgatcgg gccgatggga
      241 ttgggcttag gcgcatgctg gagccggctg cgcatgccgc cgacgcatgt ccttggactt
      301 gatccgggga atggatttgc ggttttgcgg gcggaacgcg cgcgaattat tgatttggaa
      361 cattttctgc ggcaaaccgg attgcacctt cggatcaagt ccaagggcat gctttgtcgg
      421 aagatgcgct gatgcggctc gtcgtgatct caatcggccg gctgaagcag ggcccggagc
      481 gggaattggc cgagcgctat cgcgagcgat tcgacgacat cggccgcaaa ctcggttttc
      541 gtggtcttga gatccaggaa attcccgaaa gccgcgcccg cgacgccgca acccggatga
      601 ccgacgaagc caccgctatt tcggcggcaa ttcctgctaa atcggtgctg gtggcgctcg
      661 acgaacgcgg cgacagcatc gacagcgccg cttttgcccg gcatcttggc cgctggcggg
      721 atgaacaggt cgccaacact attttcgtga ttggcggcgc ggacggactt tcgcccgatt
      781 tgcggcgcaa ggcaaaactg agcattgcgt tcggctcagc tacctggccg caccaaatgg
      841 ttcgcgtcat gcttctggaa caggtttatc gggccgccac tatattggcg ggccacccct
      901 atcaccgcgt ttgagccgcg ccagcggaag cattgaaaaa cactgattcg gactaaatgc
      961 aggcaccgcc cgacattcat tcgtaccctg acaccgcccg ccgcggtcag ctatcctcag
     1021 cccgtcttcc gctttcgatc gccttgttgt ccgcagcctt tgccggcatg agcctgttgc
     1081 cggcggcggc ccaggtcttg ccggcggcgg cccaagtctt gccggcggcg gcccaagtct
     1141 tgccggcggc gcagggcgcg gcatcgacac agcagacgac ggcctcgtca cctgacgcga
     1201 tcaaacagcg cgagcaggaa ctcgaagccg cccgcgcgca gcaaaagagc gccgcggaat
     1261 tgcagatgaa gctccagacc gatctcgcag cgatcggtca ggatcacagc aagctcaatc
     1321 agcggctgat cgacatcgcc ggacaggtgc gcagcgtcga ggccaggata ggcgacgccg
     1381 aaacgcggct gcgcccgctc gactctcgcg agcaacagat tcgcagttcg cttggttcgc
     1441 gccgttctga aattgtcgag gtgctggcta cgctgcagcg cgcgggacgg cggatgccgc
     1501 cagccttgct ggtaaggccg gaagacgcgc tgcagtcgct tcgcacggcg atgctgctcg
     1561 gatccgttgt gcccgaattg cgcggacgtg cggacagact ggccaccgac ctcggcgaac
     1621 tggtctcggt tcgcaagaac atcgctaccg agcgcgacca gcttgcggtt gatcgcgaca
     1681 aactcaggga tgaccaaacc cggctcgccg cgctcgtcga cgagcggcag cgaaaacaga
     1741 gttccatcga aaaagacatg gcggcggaaa gcgcgcgcgc catcacgctg tcccagcagg
     1801 tcgacggcct cgagggcctg atcgccaaga tggagcagga cttgaagagc gccgcgagag
     1861 cggccgcgag cgccagcctg cggggtgctc ccgttgctcc gaatggcaaa ccgaatctcg
     1921 gagggctgaa ggactccgcc cggctgagcc cggcgattgc ttttgcctcc gccaagggtt
     1981 tattcgcgtt cccggttaat ggcgtcaaga ttcgcgagtt tggcggttcc gatggttccg
     2041 gcggggttga aaaaggcatt tctttgtcga cccgggccgg agcccaagtc acaacaccgt
     2101 gtgatggctg ggttgtttac gccggaccgt ttcgcagcta cggacaactc ttgatcctga
     2161 atgccggggg cgggtatcat gtgttgatcg ccgggatgga gcgcatttcg gttaacatcg
     2221 gccagtttgt acttac
//
LOCUS       DASWGM010000306         7630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_158308,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000306 DASWGM010000000
VERSION     DASWGM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158308"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VGA15_08850"
     CDS             <1..476
                     /locus_tag="VGA15_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEX9587840.1"
                     /translation="LLLSSSLLSARAETPLPDAIAAPGEATVLTVHAEGAQVYECRAG
                     ADGKPAWAFREPIATLLVDGKTVGRHYAGPNWEHSDGSAAVGKAAGNAPGATPNDIPW
                     LKLEVISHRGSGILTAATTVQRINTIGGKLEGACDKAGAFKSAPYSADYVFLRKG"
     gene            559..843
                     /locus_tag="VGA15_08855"
     CDS             559..843
                     /locus_tag="VGA15_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEX9587841.1"
                     /translation="MLFMVIERFKDRDPIPVYKRVRDEGIKFPEGLRYVGSWIEPNFD
                     RCFQLMECDDARLLQDWILNCQGFGMTFEIVPVVPSKETREVVAPFLDSK"
     gene            1260..1526
                     /locus_tag="VGA15_08860"
     CDS             1260..1526
                     /locus_tag="VGA15_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587842.1"
                     /translation="MMRLKVETTGKGLHTSEVVVKVKTSSGVERLVVSERAIDNNEIS
                     IGWPIRTKDDLYLIELPNETQSGAWRVWVNRDQVSEPQPQRVRA"
     gene            1824..2081
                     /locus_tag="VGA15_08865"
     CDS             1824..2081
                     /locus_tag="VGA15_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587843.1"
                     /translation="MPTDARLAARVEGKSSLARLGIAVHLTAPTIHAGFKGRVRLEIV
                     NHGPLPIVLKSGMRICQFIFEMTHGTPEAGYKGQFLGQGQR"
     gene            complement(2548..5229)
                     /gene="alaS"
                     /locus_tag="VGA15_08870"
     CDS             complement(2548..5229)
                     /gene="alaS"
                     /locus_tag="VGA15_08870"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX9587844.1"
                     /translation="MSGVNEIRSAFLNYFEKNGHAIVPSSPLVPRNDPTLMFTNAGMV
                     QFKNVFTGVEKRPYQRATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KDRAIELAWNLVTKEFGLPKDKLTATVYVDDDEAFGLWKKIAGLPESRIIRIAGADNF
                     WQMGDTGPCGPCSEIFYDHGDKIWGGPPGSPEQDGDRFIEIWNLVFMQFEQIEPGVRN
                     PLPKPSIDTGAGLERVAAVLQGKHDNYDIDLFVALIRAISELTGADPKGEQKASLRVI
                     ADHLRASSFLISDGVLPSNEGRGYVLRRIMRRAMRHAQLLGATDPLMHRLVWALVREM
                     GQAYPELVRAENLIEETLRLEETRFRKTLERGLTILDEKSASLKKGDMFDGDTAFTLY
                     DTYGFPLDLTQDALKSRGISVDIASFTDAMDRQRDKARAAWAGSGDIASENVWFPLRE
                     KLGATEFLGYETETAEGVVAALVKDGKDVDSLKAGESGAIVLNQTPFYAESGGQVGDT
                     GVMAGEGVKFRVTDTQKKAGDLFVHLGTVEQGTLKPGTALLLDVDHSRRASIRAHHSA
                     THLLHEALRQVLGDHIAQRGSLVAPDRLRFDFVHQKPITQDELARVEDIANNVVLEND
                     EVTTRLMAVDDARDAGARALFGEKYGDEVRVVSMGKTDSEHGSNALGWSVELCGGTHV
                     RRTGDIGLISITGESAVASGVRRIEALTGNHARKHANDTIALAKTAATELRTTLEDMP
                     ARIASLMEERKKLERDLSDARKKLAMGGAATTNGSAAGASGVREVGNVKLMARSVEGI
                     EMKDLKSLVDDGKKQIGSGVVAIVAVTEDGKAGVVVGVTGDLTSRYNAVDLVRVASEA
                     LGGKGGGGRPDMAQAGGPDGANASAAIAAIEKAMGSG"
     regulatory      5543..5634
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5775..6923
                     /gene="gcvT"
                     /locus_tag="VGA15_08875"
     CDS             5775..6923
                     /gene="gcvT"
                     /locus_tag="VGA15_08875"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX9587845.1"
                     /translation="MLAREPETSLKRTPLHALHVASGGKMVPFAGYDMPVQYTTGVLK
                     EHLHTRQAAGLFDVSHMGQIALRAKSGKVEDAALALERLVPQDILGLAPGRQRYAQFT
                     NGAGGLLDDLMVANFGSHLFLVVNAACKAEDEAHLRANLSETCVIEQLPDRALIALQG
                     PKACSVLAKFCADAAAMRFMDSGPRSIDGIDCFVSRSGYTGEDGFEISVPADHAERLA
                     KALLGDSDVLPIGLGARDSLRLEAGLCLYGHDIDTTTTPVEGALEWSIQKIRRHGGAR
                     AGGFPGAETILSQLAMGAPRRRVGLRPEGRAPVREGAPLFADAASSLRIGAVTSGGFG
                     PSINTPVAMGYLPASHAAIGGLVFAELRGQRLPLRVAAMPFVPNTYKR"
     gene            6948..7313
                     /gene="gcvH"
                     /locus_tag="VGA15_08880"
     CDS             6948..7313
                     /gene="gcvH"
                     /locus_tag="VGA15_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX9587846.1"
                     /translation="MTTLFTPDHEWLRIEGDVATIGVTDYAQSQLGDVVFVELPKVGR
                     SLKKAEAAAVVESVKAASDVYAPISGEVTEVNDAVVAEPALVNSEAAGKAWFFKLKIS
                     DKSELDGLMDEAAYAKHTA"
BASE COUNT         1492 a         2350 c         2294 g         1494 t
ORIGIN      
        1 tgctcctgct gtcaagctca ctcctgagcg cgcgggccga gacgccgctt cccgacgcca
       61 ttgcggcgcc gggagaagcg accgtgctta ccgtgcacgc cgaaggcgcg caggtctacg
      121 aatgccgggc cggcgccgac ggcaagccag cctgggcatt tcgcgagccg attgcgaccc
      181 tgctggtcga cggcaagacc gtcggccgcc actatgccgg accgaactgg gaacacagcg
      241 atggcagcgc cgcggtcggc aaggccgccg gtaacgctcc gggcgcgaca ccgaacgata
      301 ttccctggct caagctcgag gtgatttccc accgcggcag cggcatcctc accgccgcca
      361 ccacggtgca gcggatcaac acgataggcg gcaagcttga gggagcctgc gacaaggccg
      421 gcgccttcaa gagcgcgccc tactccgccg actatgtgtt cctgcgcaag ggctgagcgg
      481 caaggcctaa gcgacaaggc ttgaccggcg aggcgtcctg aacaacgctg cacgtcaccg
      541 atcgataagg ggttgccgat gctgttcatg gtgatcgagc gcttcaagga ccgtgacccg
      601 atcccggtct acaagcgcgt gcgcgacgag ggcatcaaat ttcccgaagg actgcgctat
      661 gtcgggagct ggatcgaacc caatttcgac cgctgctttc agctgatgga atgcgacgac
      721 gcgcggctgc tgcaggattg gatcctgaac tgccagggtt tcggcatgac cttcgagatc
      781 gtcccggtgg tgccgagcaa ggaaacccgg gaagtcgtcg ctccgtttct ggattcgaag
      841 tagcgccgcg ggccggttct ttcgggcatc cggtgaacct tggcagctct ctacagacag
      901 atagctaccg ggggattgca ctgcccggtt cttgccggcg ccgccaagca ttattcagcc
      961 tggcggcgtc gtgcgtttgg gggcccggtt ttgagcggct tttggccggt acgaccaatg
     1021 atattcttta tttgttctgg ctctgtggtt catttagatg aatggaacat ctcattcata
     1081 ttcgttaatt cgtagtggct gataggcagc aacttcaaaa aagttaactt tatccccagg
     1141 atgttcttta tttgttctca attcggagta agttgttaac cagcgggcgt cggatttagc
     1201 cgacaattgt tagctgacga atcgcacttg atttaagcaa gaagaacggg agataatcca
     1261 tgatgcgcct caaagtagaa accaccggaa aaggacttca tacgagcgag gtcgttgtaa
     1321 aggtaaaaac ttcaagcggc gtcgagcgtt tagtggtttc tgagcgcgca attgataata
     1381 acgaaattag tataggttgg ccgataagaa ccaaagatga cctatatcta atcgagcttc
     1441 ccaatgagac ccaatctggc gcgtggagag tttgggtaaa tcgtgaccag gtatctgagc
     1501 ctcaaccaca acgggtaaga gcttgattct cactgaccgc gaaattcaga tttcgttgga
     1561 acgaaaactc atttttattg agcctcttcc atcggaagag gcctttagct ccacgagcgt
     1621 agatctaacg cttgatgcgg aaatcacgcg cttcaatgcg aacccgcaat ttatgcggca
     1681 agccatcgat gttacacatc aagactatga ctacgaagtc gcgctagctc aactagctaa
     1741 gcactaagag ccaaattacg gccaccggct ttgaactacc tcgaaacgaa cttattcttg
     1801 ggtggacggc ggaatatata aacattccaa cggatgcgcg attggcggcc cgcgtcgagg
     1861 ggaagagttc actcgcccgc ttaggaattg ccgttcatct tacagcccca actattcatg
     1921 ccggcttcaa gggcagagtt cggttagaaa tagtcaatca tggccctctt cctatagtcc
     1981 tcaagtctgg catgagaatc tgtcagttta tatttgaaat gactcacggg acgcctgagg
     2041 ccggctacaa aggccaattt ctaggtcaag gtcaaagata atcagactag gctgccgttc
     2101 gcttgcgtcc tcacgggcgt cgatcagcgc gacgatattt tccatgctcc aaagcttgtc
     2161 tgtgactcct gccgccattg ctcgatcagc gagtaaggtt tgttcgtcaa aattcaggaa
     2221 tgtggtcaaa actgacgaca gctatcggca ccggacatga ttgcctggtt gtggatgtcg
     2281 cgggccgctc tgcggcgttc gaatcagatg gcccctgcgt ggtcagccca acgcgggggc
     2341 cgtttctgtt gccaactatt ttgaaagatg agcgatagtg accagctgcc tcaggcgccg
     2401 agggagcatg ctggcccgat catcgcgcgg ccagatcagg cacgcgctcg atcagcgcgt
     2461 gaaaatcctg cgcatcgaac gccagcagtt tcgtttttcg cgtcgccgtc acggtgccgc
     2521 tgcgaagcga atttcagatc catcgcatca gccgctgccc attgcctttt cgatcgccgc
     2581 gatcgccgca ctggcgttgg cgccatccgg cccgccggcc tgcgccatgt cgggccgccc
     2641 gccgccgccc ttgccgccga gcgcttcgga ggcgacccgc accagatcga ccgcattgta
     2701 gcgcgaggtg agatcgccgg tgacgccgac cacaacgccg gccttgccgt cctcggtgac
     2761 ggccacgatg gcgaccacgc cggagccgat ttgtttcttg ccgtcatcga cgaggctctt
     2821 gaggtccttc atttcgatgc cctcgaccga acgcgccatc agcttgacgt tgccgacttc
     2881 gcgcacgccg ctggcgcccg ccgccgaccc attggtggtg gcagcgccgc ccatcgccag
     2941 tttcttgcgc gcatctgata gatcgcgctc cagcttcttg cgctcttcca tcagcgaggc
     3001 gatgcgcgcc ggcatgtcct cgagcgtggt gcgcaattcc gttgccgcgg tcttggcaag
     3061 cgcaatggta tcgttggcat gctttcgcgc gtggttgccg gtcagcgcct cgatgcggcg
     3121 cacgccggag gcaaccgcgc tttcgccggt gatcgagatc aggccgatat cgccggtccg
     3181 gcgcacatgg gtaccgccgc acaattccac cgaccagccg agcgcgttgc tgccgtgctc
     3241 ggaatcggtc ttgcccatcg acaccacccg gacctcgtcg ccatattttt caccgaacag
     3301 cgcgcgggcc ccggcgtcgc gggcgtcgtc caccgccatc agccttgtcg tgacctcgtc
     3361 attctccagc accacattgt tggcgatgtc ctcgacgcgg gcgagctcgt cctgggtgat
     3421 cggcttttga tgcacgaagt cgaagcgcag ccgatccggc gccaccagcg agccgcgctg
     3481 ggcgatatgg tcgccgagca cctgccgcag cgcctcgtgc agcagatgcg tcgcggagtg
     3541 atgggcgcgg atcgaggcgc gccttgagtg atcgacgtcg agcagcagcg ccgtccctgg
     3601 cttcagcgtg ccctgctcca cggtgccgag atgcacaaag agatcgccgg ccttcttctg
     3661 ggtatcggtg acgcggaatt tcacgccctc gccggccatc acgccggtat cgccgacctg
     3721 accgccggac tccgcataaa acggcgtctg gttcagaaca atagcgcccg actcgccggc
     3781 cttgaggctg tcgacatcct tgccgtcctt caccagtgcc gccaccacac cttcggcagt
     3841 ctccgtctcg taacccaaga attcggtggc gccgagtttt tcgcgcaacg gaaaccagac
     3901 gttctcgctg gcgatatcgc cggagcccgc ccaggccgcc cgcgccttat cgcgttggcg
     3961 gtccatcgcg tcggtaaagg acgcaatgtc gacgctgatg ccgcgcgatt tcagcgcgtc
     4021 ctgcgtcaga tcgagcggaa agccataggt gtcgtacagc gtgaacgcgg tgtcgccgtc
     4081 gaacatgtcg ccttttttca agctggcgct cttctcgtcg agaatggtca gcccgcgctc
     4141 cagcgtcttg cggaagcgcg tctcttccag ccgcagcgtt tcctcgatca ggttttcggc
     4201 gcgcaccagt tcgggatagg cctggcccat ctcgcgcacc agcgcccaga ccagccggtg
     4261 catcaagggg tccgtggcgc cgagcaactg cgcgtggcgc atcgcccggc gcatgatcct
     4321 gcgcagcacg tagccgcggc cttcgttcga cggcagcacg ccgtcggaaa tcaaaaacga
     4381 cgacgcgcgc agatgatcgg cgatgacgcg caacgaggcc ttctgctcgc ctttcggatc
     4441 ggcgccggtc agttcggaga tggcgcggat cagggctacg aacagatcga tgtcgtagtt
     4501 gtcgtgcttg ccctgcaaca ccgccgcgac acgctccagc ccggcgccgg tgtcgatcga
     4561 gggtttcggc agcggattgc gcacgcccgg ctcgatctgt tcgaactgca tgaacacgag
     4621 attccagatc tcgataaaac ggtcgccgtc ctgttcaggc gaacccggcg gtccgcccca
     4681 gatcttgtcg ccgtggtcgt agaaaatctc cgagcagggg ccgcaaggtc cagtgtcgcc
     4741 catctgccag aaattgtcgg cgcccgcgat gcggatgatg cgggactcgg gcagacctgc
     4801 gatcttcttc cagagaccaa acgcctcgtc gtcatcgaca tagacggtcg ccgtgagctt
     4861 gtcctttggc aggccgaatt ctttcgtcac cagattccac gcgagctcaa tggcgcgatc
     4921 cttgaaataa tctccaaatg aaaaattgcc gagcatctcg aagaacgtgt gatgccgcgc
     4981 cgtatagccg acattgtcga gatcgttgtg cttgccgccg gcgcgaacgc atttctgcga
     5041 cgtggtggcg cgctgatagg gacgtttttc gaccccggta aagacgttct tgaattgcac
     5101 catgccggca ttggtgaaca tcaaggtcgg gtcgttgcgc ggcaccagcg gtgacgacgg
     5161 cacgatcgcg tggccgttct tctcaaaata gttcaggaac gccgatctga tttcgttaac
     5221 gccgctcatg tcagtccaat cgcaaaacca ttggggattc gccgcaaacg gggccttttc
     5281 agcgcatgat ccgccaaagt gtgaagcggt ttggcgaaga gatcatgcgc ccgttcaaca
     5341 attagagcgc ggccggacgc aaaaccggaa tccacttttg ctgaccgcgc tctaccgcaa
     5401 cgcttttaga cgcgggggct ggcggtgtcc agaaagtgtg cagcggatca tgggcttgcc
     5461 gcaccagcac aggaaccgtt gatatcagcg caagccgcgt tgacaggctt gatgcagccg
     5521 tgttttcctc ctatctgttc aagacagtcg cgtcgggaga gatcggcttt aagagccggc
     5581 gccgaaggag caaccgcccc ggaaactctc aggcaaacgg accgcgtgac tggctagcat
     5641 ctggaaagag acgccgaagg gcttgaagac cctgacgtgt ccgccgacgg gataatactc
     5701 tcaggcacag cgacagatgg ggcttcgaca ggtttctcga ggaatcaccc ttgaagaacc
     5761 gcgagggccc catgatgctt gcgcgcgagc ccgaaacatc gctgaaacgc acgcctttgc
     5821 acgcgctgca tgtcgcctcc ggcggcaaga tggtgccgtt cgcgggctat gacatgccgg
     5881 tgcaatacac aaccggcgtg ctcaaggaac acctgcatac ccgccaggct gccggcctgt
     5941 tcgacgtttc gcatatgggc cagatcgcgc tgcgggcgaa atccggcaag gtcgaggacg
     6001 cagcactggc gctggagcgg ctggtgccgc aggacatcct tggcctcgcg ccgggacggc
     6061 agcgctacgc gcagttcacc aacggcgcgg ggggccttct cgacgatctg atggtggcga
     6121 atttcggcag ccatctgttt ttggtggtca acgccgcctg caaggccgag gacgaggcgc
     6181 atctgcgcgc gaatctctcc gagacttgcg tgatcgagca gcttcccgat cgggcgctga
     6241 tcgcgctgca ggggccgaaa gcctgttccg tgctggcgaa attttgtgca gacgcggctg
     6301 cgatgcggtt catggattcg ggcccgcgaa gcatcgacgg catcgattgc tttgtctcgc
     6361 gctcgggcta caccggcgag gacggttttg agatttcggt cccggccgat cacgccgagc
     6421 ggctggcgaa ggcgctgctg ggggatagcg acgtgctgcc gatcggattg ggcgcccgcg
     6481 acagcctgcg gctcgaggcc gggctttgtc tgtatggcca cgacatcgac accaccacga
     6541 cgccggtcga gggcgcgctg gaatggtcga tccagaagat ccggcgccat ggcggcgccc
     6601 gcgccggcgg ttttcccggt gcggaaacga ttctctcgca gcttgcgatg ggcgcaccgc
     6661 gccgccgcgt cgggttgaga ccggaaggcc gcgccccggt acgcgaaggc gcgccgttat
     6721 tcgcagacgc cgcgtcatcc ctgcggatcg gcgcggtcac ctcgggcggc tttggcccca
     6781 gcatcaatac gccggtcgcg atgggctatc tgcccgcctc gcacgccgcc atcggcggcc
     6841 tggtattcgc cgaattgcgc ggacagcgcc tgccgctgcg ggtcgcagcc atgcccttcg
     6901 ttcccaatac ctacaaacgc tgacttgcaa acgctagagg atttgtcatg accacgctgt
     6961 ttacccccga ccacgaatgg ctccgcatcg aaggcgatgt cgccaccatc ggcgtcaccg
     7021 attatgcgca gtcgcagctc ggcgatgtcg tgttcgtcga actgccgaag gtcggccgca
     7081 gcctgaagaa agcggaagcc gccgccgtgg tcgaatccgt caaggccgca tccgacgtct
     7141 acgcgccgat ttcaggcgag gtaacggaag tcaacgacgc agtcgtcgcg gagccggcgc
     7201 tggtcaattc cgaagccgcc ggcaaagcct ggtttttcaa gctgaagata tcagacaaga
     7261 gcgaactcga cggcctgatg gatgaggcgg cgtatgcaaa gcacaccgcg tgagatgatc
     7321 aagaaaaccc agacaccaaa ctcagcgtca tcgtccgcga aagcggacga tccagtacgc
     7381 cgtgacgttc cggttcaacc actaatgtca cggaatactg gatgccccga tggagcctgt
     7441 catcgggctc gccgaaggcg agacccggtg gctgggcatg acgacccagc gaggaacacc
     7501 ccatgaccat gcaccacaaa cccgccaatg gcgtcgccac cagcttcgcg cgccgccaca
     7561 tcggcccctc gccgcgtgac atttcagcga tgctcgaaac ggtgggcgca aagagcctca
     7621 gcgcgctgat
//
LOCUS       DASWGM010000307         4364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_158392,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000307 DASWGM010000000
VERSION     DASWGM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158392"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VGA15_08885"
     CDS             <1..489
                     /locus_tag="VGA15_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9587847.1"
                     /translation="LLQLEVTESMVMRNVSRAVKVLDAIQRRGIRLAIDDFGTGYSSM
                     SLMKQFPIDTIKIDRSFVRDLPDDSEDKAIAQAIISMGKALGMTVVAEGVETAEQQTF
                     LRDHACDEMQGFLFSKPLPPQQLAELLRPMALVVAPPLQPAAIVAPEKSTPRSRRRSA
                     AV"
     gene            complement(635..928)
                     /locus_tag="VGA15_08890"
     CDS             complement(635..928)
                     /locus_tag="VGA15_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587848.1"
                     /translation="MHQAANVQFGRAVGEFASWRAVPEDARSPAPAWWWGPAFEVLGA
                     PQPMPAEWCAKLELPEGATYATGAAIFLVALAGQTSLPWPGEFPRKAGHSDVA"
     gene            complement(1008..2549)
                     /locus_tag="VGA15_08895"
     CDS             complement(1008..2549)
                     /locus_tag="VGA15_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587849.1"
                     /translation="MVGFIRPPCDAGIIRATTAVPVALSRRRKQLTLAATILGSSLAL
                     IDGSVVNIALPAIQQALHADAASTQWIVNAYLLLLGALVLVGGSAADLYGRRRIFLLG
                     IAIFTSASIACGLSPDIMVLVVSRAVQGFGAALLTPASLAMLGATFDEHERSHAIGIW
                     AGAGALTAAAGPVLGGWLVDHVSWRAIFLINVPLAIAAAGLAIFFACESRDDQAKPLD
                     WNGAVAVAIGLAAITWGLGAIPSLGFRDKAVLGALGTGIASLLSFVAIEARSCERAMM
                     PLALYRSRNFSGTNMLTLLLYFALGGALYFLPFGLIRLGGYSATQAGAALLPFALIMG
                     FGASFAGRVADRFGPRLSLTAGPIIAAGGLALLAFADLGKSYWAGVFPAIGVLSIGMT
                     ITVPPLTSTVMASVGDARAGIASGVNNAIARVAGLFAVAALGAVLFASFSRHLVGITP
                     AGANEALNAVLAGQAEVVAGATAAFDRALRTVMLVTAVCAALGGIAGWLWVRPSDPAE
                     PRNIS"
     gene            complement(2651..3181)
                     /locus_tag="VGA15_08900"
     CDS             complement(2651..3181)
                     /locus_tag="VGA15_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX9587850.1"
                     /translation="MPSSESALVVLVPEAEAVAKPFRDQYDPSAAAGMPAHVTLLYPF
                     KAPDEIDEITLCKLRDCFARFEPIAFSLRIIQRFPVGVLYLAPEPDEPFRQLTLSICN
                     LFPETPPYGGKWPDIIPHLSVAQLANEQRLTAIAEDFAKTSQGELPIHAFASEVALID
                     NRSGRWKVRVMFGLGT"
     gene            complement(3279..3836)
                     /locus_tag="VGA15_08905"
     CDS             complement(3279..3836)
                     /locus_tag="VGA15_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9587851.1"
                     /translation="MILSGRLRLRNWRESDRDAFAGLNAAPEVTQDLGGPLDRNESDA
                     KFDRYVAAFDQCGFCRWALEDLDGQFLGYAGVMPSRPGHPLGPHADIGWRLVRPAWGR
                     GYATEAARASLRDVFERVNLKKVLAYTSADNVRSQAVIKRLNMQRAATLDYSEPLGKG
                     AWHGLMWMVRRGDLVDLDHSDDRAR"
     gene            complement(3866..>4364)
                     /locus_tag="VGA15_08910"
     CDS             complement(3866..>4364)
                     /locus_tag="VGA15_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9587852.1"
                     /translation="SSRNSTEEIMKATNRFWRYLAMPICFAGMLSISSAAELNPAAVI
                     YKLPDQIPWSPVDARGAQNAVVVGDPTKPGFYAVYTKWTKGNHFSRPHFHPNDRYIVV
                     LQGTWWVGSGPKFDPEKGTTPMPAGSFVTHFGKQVHWDGAKDEDAVLLIMGEGPATST
                     AAEEK"
BASE COUNT          885 a         1377 c         1342 g          760 t
ORIGIN      
        1 ctgctgcagc ttgaggtcac cgaaagcatg gtgatgcgca acgtttcccg ggcggtcaag
       61 gtactggacg ccatccagcg tcggggcatt cgtcttgcga tcgacgattt tggaaccggc
      121 tattcgtcaa tgtccctgat gaagcaattt ccgatcgaca ccatcaagat cgaccgttcc
      181 tttgttcgcg accttccgga tgactccgag gacaaggcca tcgcgcaggc gatcatcagc
      241 atgggcaagg cactggggat gacggtcgtt gcagaaggcg tggagaccgc cgagcagcaa
      301 acattcctgc gcgatcacgc ctgcgatgaa atgcagggct tcctgttcag caaaccgctg
      361 ccgccacagc agctggccga actgctccgg cccatggcgc ttgtcgtcgc tccgccgctt
      421 cagcctgcgg caattgttgc gcctgagaaa tccaccccac ggtcacggcg gagaagtgcg
      481 gccgtctaga gccatttccg tttcgataga atcgaaacgg ggctctatag attcttgatt
      541 tgacgcgttt tcttgacgcg aaccggtcgc caccccggat caagtccggg gcaggctttc
      601 gctggaaaac gctttagcaa gctctttgat cgcgctaggc cacgtctgaa tgccctgcct
      661 tgcgtggaaa ttcgccgggc cagggcagcg acgtctggcc ggcgagcgcg acgagaaaaa
      721 ttgcagctcc cgttgcgtaa gtggcaccct cgggcaattc cagctttgcg caccactccg
      781 ccggcatcgg ttgcggcgcg ccgagcacct caaacgccgg tccccaccac catgccggcg
      841 ccggcgatct cgcgtcttcc gggacggcgc gccagctggc gaattcgccc acggcgcgtc
      901 cgaattgcac atttgcggcc tgatgcatcg aaatcctccg gcgcttcgat gatacgcgta
      961 tctgtagccg acgcaacgcg tgccgatgaa atttactgcc cgcgatatca ggaaatgttg
     1021 cgcggctccg ccgggtcgga cggccgcacc cacagccagc ctgcgatgcc gccaagcgcg
     1081 gcgcagacgg ccgtcaccag catcaccgtg cgcagtgcgc ggtcgaacgc tgccgttgcg
     1141 ccggcaacaa cttcagcctg tccagccagg accgcattca atgcctcgtt ggcgccagcg
     1201 ggtgttattc cgacaaggtg acgcgaaaag ctggcaaata aaacggcgcc gagtgccgcg
     1261 actgcgaaga gaccggcgac gcgggcaatg gcattgttga cgccggaagc gataccggcg
     1321 cgagcgtcac ccaccgatgc catcactgtc gacgtcagcg gcggaacagt gatggtcatg
     1381 ccgatcgaga gcaccccgat cgccggaaac acgccggccc agtaggattt tccgaggtcg
     1441 gcaaaggcga gcagtgccaa tccgccggcc gcgatgatgg gccctgcggt caacgacagc
     1501 cgcggcccga aacggtcggc gaccctgccc gcgaacgacg cgccgaaccc catgatcagc
     1561 gcgaacggca gcagcgccgc gccggcctgc gtcgcggaat agccgcccag ccgtatcagg
     1621 ccgaagggga gaaaatacag cgcgccgccg agggcgaagt aaagcagcag cgtcagcatg
     1681 ttggtgccgg aaaaattgcg cgagcggtac agcgccagtg gcatcatcgc gcgttcgcag
     1741 gagcgggctt cgatcgcgac gaatgacagc agcgacgcta ttcccgttcc cagcgcgccg
     1801 agtacggcct tgtcgcgaaa tcccagggat ggaatcgcgc ccagccccca ggtgatcgcg
     1861 gccaacccga tggcgaccgc gactgcgccg ttccagtcca gcggttttgc ctggtcgtcg
     1921 cggctttcgc aggcgaagaa gattgcaagc ccggccgcgg cgatagccaa cggcacgttg
     1981 atcaggaaga tcgcgcgcca cgacacatga tcgaccagcc agccgccgag cacgggaccc
     2041 gccgccgccg tcagcgcgcc ggcacccgcc cagatcccga tcgcatggct gcgctcatgc
     2101 tcgtcgaaag tggcaccgag catcgccaga ctggccggcg tcagcagtgc ggcgccaaaa
     2161 ccttgaaccg cgcggctgac gacgagcacc atgatgtcag gtgagagtcc gcaggcaatc
     2221 gaggcggagg tgaatatcgc aatgcccaga agaaaaatcc gccggcggcc gtaaagatcg
     2281 gcagccgagc ccccgaccag caccagcgcg ccgagcagca acaggtaggc gttgacgatc
     2341 cactgggtgg aagctgcgtc cgcgtgcagc gcctgctgga tcgcgggcag ggcgatattc
     2401 accaccgaac cgtcgataag cgccaggctt gagccgagga tcgtcgccgc cagcgtcaat
     2461 tgcttgcgcc gtcgcgatag cgcgacggga acagccgtcg tcgcgcggat gattccggcg
     2521 tcgcagggcg gccgtatgaa accaaccata tggtcctcag gggagcgtgg ctaacaagct
     2581 gcgatggtgc gcttcgtgcg ctccaatggc aacgcgccac gcgcgatagg gggccgtggt
     2641 cggtcatcgc tcatgtcccc aagccgaaca tgaccctgac tttccaacgc ccggaccggt
     2701 tgtctatcag agcgacctcg gacgcgaatg catggatcgg caactcaccc tgagatgtct
     2761 tcgcgaaatc ctcggctata gcggtgagcc gctgttcgtt cgcaagctgc gccaccgata
     2821 gatgggggat aatgtcgggc cactttccgc cataaggagg cgtttccggg aacaggttgc
     2881 aaatcgacaa ggtgagttgg cgaaatggct catccggctc cggggccaaa tacaatacgc
     2941 cgacggggaa acgctggatg atgcggagcg agaacgcaat cggttcaaag cgcgcgaagc
     3001 agtcgcgtag cttgcaaagg gttatctcat cgatttcatc gggtgctttg aatggatata
     3061 gtaatgtgac gtgcgctggc atcccggcag cagctgaggg gtcgtattga tcacggaacg
     3121 gcttcgcgac cgcctccgct tcaggcacca acaccaccag cgcggattca ctcgacggca
     3181 tatcgtttag tatccatcat aaacacgaag tacggaatac ggctcatgtt atgcgcagaa
     3241 ggacgtgcgc gcatcccaca cgttacctgg aatatcgatt aacgggcgcg gtcatccgaa
     3301 tgatccagat cgactaagtc gccccgtcga accatccaca tcaagccgtg ccacgctcct
     3361 ttgccgagtg gctcggaata atcaagtgtc gcggcacgct gcatgttcaa tcgcttgatc
     3421 accgcttggg atcggacatt gtccgccgag gtataagcga gcactttttt caaattcacc
     3481 cgctcgaaga cgtcccgcaa ggatgctctc gcagcctcgg tggcatagcc ccgaccccag
     3541 gccgggcgca cgaggcgcca gccgatgtcg gcatggggtc cgagcggatg accgggccgc
     3601 gacggcatga cgcccgcata gcccaggaat tgcccgtcca gatcttcgag ggcccatcgg
     3661 cagaagccgc attggtcaaa tgccgccacg taacggtcaa atttggcgtc gctctcgttg
     3721 cgatccaacg ggccgcccaa atcctgcgtg acctcgggag cggcattgag gccagcaaac
     3781 gcgtctctgt cggactcccg ccagttccga agtctgagcc ggccagaaag gatcaccgct
     3841 cgttactccg cagaggctga ggccgttatt tctcttccgc ggccgtcgag gtcgccggcc
     3901 cttcgcccat gatcaaaagc acggcatcct catccttggc gccatcccaa tgcacctgct
     3961 tgccgaaatg ggtgacgaaa cttccggcag gcattggcgt agtacccttt tcgggatcga
     4021 attttggtcc cgagccgacc caccaggtgc cctgcagcac gacgatgtaa cggtcgttgg
     4081 gatggaagtg cggccggctg aaatggttgc ccttggtcca tttggtgtag acggcataga
     4141 agccgggctt ggtcgggtcg ccgacaacga cggcgttttg cgcgccgcgg gcgtcgaccg
     4201 ggctccacgg gatctggtcc ggcagcttgt agatcaccgc ggccggattg agttcggccg
     4261 ccgagctgat gctcagcatg ccggcaaagc atatcggcat cgccaggtat cgccaaaacc
     4321 ggtttgtggc cttcatgatt tcctccgttg aattccgcga gctg
//
LOCUS       DASWGM010000308         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1968191,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000308 DASWGM010000000
VERSION     DASWGM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968191"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VGA15_08915"
     CDS             <1..295
                     /locus_tag="VGA15_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX9587853.1"
                     /translation="TQKEEKNTPIPVVITIYADRSFTFEMKTPPMSFFLKQAAKIQSG
                     SKAPGRDKAGKVTKAQVREIAEKKMKDLNCDTIEAAMKMVEGSARSMGVEVAG"
     gene            301..993
                     /gene="rplA"
                     /locus_tag="VGA15_08920"
     CDS             301..993
                     /gene="rplA"
                     /locus_tag="VGA15_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX9587854.1"
                     /translation="MAIGKRLKKAREGVDREKLYPLADAIKMVKERAKSKFDETVEIA
                     INLGVDPRHADQMVRGVVTLPNGTGRTLRVGVFARGAKAEEAKAAGADVVGAEDLVEK
                     VQGGQIDFDRCIATPDMMPLVGRLGKVLGPRGMMPNPKIGTVTMDVATAVKGAKGGSV
                     EFRVEKAGIVQAGIGKASFSEDKLVQNVKALADAVAKAKPAGAKGTYIQRVAVSSTMG
                     PGVKVEPGSVLG"
     gene            1081..2028
                     /locus_tag="VGA15_08925"
     CDS             1081..2028
                     /locus_tag="VGA15_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX9587855.1"
                     /translation="MPDKVLIYSRFPKAMMVRIGQRFELMDAAGKPPGEVFTAEQLSG
                     VSAMITAGGTPLGAAMMDTMPKLAAIVCYGTGYDGVDLAAAAQRGIAVGHSPAANASA
                     VADLAVTSMLAVTRRLLPADEYVRSGNWSAAKPSPLMSPQPGNPGRRVGVFGMGAIGR
                     KIASRVAAFETEVGYFSRSRHDVPYQYLPSLAALAEWCSVLMIAVRAGVDTHHAVNAS
                     ILQKLGKDGYVVNISRGSVIDQRALIAALTDKTIAGAGLDVYEKEPHAPDALTAFPNV
                     VLTPHIGGHTIESHMAMQDCVIANLEAYFAGKPLAHPVG"
     gene            complement(2065..>2318)
                     /locus_tag="VGA15_08930"
     CDS             complement(2065..>2318)
                     /locus_tag="VGA15_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272148.1"
                     /note="Member of the extracytoplasmic function sigma
                     factors which are active under specific conditions; binds
                     with the catalytic core of RNA polymerase to produce the
                     holoenzyme and directs bacterial core RNA polymerase to
                     specific promoter elements to initiate transcription;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HEX9587856.1"
                     /translation="AALNPIMGADKVARFFIALAAKNAGRDVRIQPTMINGTVGALLY
                     MDGEVDHTLSMAIDGDRIAAIYIVRNPDKLRHAPACEHH"
BASE COUNT          453 a          701 c          769 g          395 t
ORIGIN      
        1 gacgcagaag gaagaaaaga ataccccgat tccggtggtg atcacgatct acgccgatcg
       61 ttcgttcacc ttcgagatga agacgccccc gatgtcgttc tttctcaagc aggccgccaa
      121 aatccagtcc gggtcgaaag ccccgggccg cgacaaggcc ggcaaggtga ctaaagcgca
      181 ggtgcgcgag atcgccgaga agaagatgaa ggatctgaat tgcgacacca tcgaagcggc
      241 catgaagatg gtcgagggct cagcccgttc gatgggcgtc gaggtggcgg ggtaacggtc
      301 atggcaattg gaaaacgttt gaagaaggcc cgcgagggcg tcgatcgcga aaaactttat
      361 ccgctcgccg acgccatcaa gatggtcaag gaacgcgcca agtcgaagtt cgacgagacg
      421 gtcgagattg cgatcaatct cggcgtcgac ccgcgtcatg ccgatcagat ggtgcgcggc
      481 gtggtgacct tgccgaacgg cactggccgc accctgcggg tcggcgtgtt cgcgcgcggc
      541 gccaaggccg aagaagccaa ggcggcaggt gcggacgtgg tcggcgcgga ggatctggtc
      601 gagaaggtgc agggcggtca gatcgatttc gaccgctgca tcgccacccc cgacatgatg
      661 ccgctagtcg gccggttggg taaggtgctc ggcccccgcg gcatgatgcc caatccgaag
      721 atcggcaccg tcaccatgga cgttgccacc gccgtgaaag gcgccaaagg cggttcggtc
      781 gaattccgtg tcgaaaaagc cggaatcgtc caggccggca tcggcaaggc gtcgttctcg
      841 gaagacaagc tggtgcaaaa cgtcaaggcg ctcgcggacg cggtcgccaa ggcgaagcct
      901 gctggcgcca agggcaccta catccagcgc gtcgcggttt cctccaccat ggggccgggc
      961 gtaaaggttg agccgggcag tgtgctcggc taaaactttc gaaaaccgag ccagaggaac
     1021 cagggacgga attgggcaac cgattccgtc ccttatcttt gatatcaggg aaatcagtcg
     1081 atgccggaca aggtcttgat ctattcacgc ttccccaagg cgatgatggt gcgcatcggc
     1141 caacggtttg agctgatgga cgccgcgggc aaaccgccgg gtgaggtttt caccgcggaa
     1201 cagctctccg gcgtcagcgc catgatcacc gccggcggaa cgccgctggg ggctgcgatg
     1261 atggatacga tgccgaagct cgcggcgatc gtctgctacg gcaccggcta cgacggcgtc
     1321 gatcttgccg cagcggctca gcgcggaatc gccgtcggcc acagtccggc ggcgaatgcc
     1381 tccgccgttg ccgatctggc ggtgacctcg atgctggccg tgacgcggcg cttgctgccg
     1441 gcggatgaat atgtccgaag cggaaactgg tcagcggcaa aaccgtcgcc gctgatgagc
     1501 ccgcagcctg gcaatcccgg ccggcgcgtc ggcgtcttcg gcatgggcgc gatcggccgc
     1561 aagatcgcat cgcgggtcgc cgccttcgag accgaggtcg gctatttcag ccgcagcagg
     1621 cacgacgtgc cctaccagta tctgccgagc ctcgcggcgc tggcggaatg gtgcagcgtc
     1681 ctgatgattg cggtgcgtgc cggcgtggac acccatcacg ctgtcaatgc cagcatcctg
     1741 cagaagcttg gcaaggacgg ctacgtcgtc aacatctcgc gcggctcggt gatcgaccag
     1801 cgggccctga tcgcggcgct caccgacaag accatcgccg gcgccggtct cgacgtgtac
     1861 gagaaggagc cgcacgcacc ggacgcgctg accgcttttc caaatgtcgt gctgaccccg
     1921 catatcggcg gccataccat cgagtcgcac atggcgatgc aggattgcgt gatcgccaat
     1981 ctcgaggcgt attttgccgg aaagccgctc gctcatcccg tgggctgacg cgcccgatca
     2041 gtgtgggcaa caggatccga acgttcagtg gtgttcacag gctggcgcgt gacgcaactt
     2101 gtcgggattg cgcacgatgt agatcgccgc gatccggtcg ccatcgatgg ccatgctcag
     2161 cgtgtgatcg acttcgccgt ccatatagag cagggcgccc acggttccgt tgatcatcgt
     2221 cggctggatg cgaacatcgc ggccggcgtt cttggccgcg agagcgatga agaaacgcgc
     2281 gaccttgtcg gcgcccatga tcgggttcag cgctgcgg
//
LOCUS       DASWGM010000309         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1693907,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000309 DASWGM010000000
VERSION     DASWGM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693907"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VGA15_08935"
     CDS             <1..1094
                     /locus_tag="VGA15_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9587857.1"
                     /translation="PLDAGFAAVVDPSVYMRSIKIMIDDPDTDIVIIDAELPKAPHEL
                     RERNLRIVDGMAAAASKPVVYISTMSIGFTEFTKGLRKSLPHIAVMQGLDRAVGAIKS
                     LIDYASLRKEVPELVSSSSASARAMLEKILNNARGAALDEVASKKLLKAYGIPVSREE
                     IAETAADAVKIAKKIGFPVVAKVVSADILHKSDIGGVVLNINSAAEVKKAFNDITARV
                     KKLKGKPKLEGILIAQQVKAELELVVGASLDAEMGPVVLFGTGGVDIELMKDVALAGA
                     PLDEADARELINRTKAGVKLKGYRGKPALHEASAIKAIVGLSNLMADAGTRIASIDVN
                     PFLINTRTGVAVDGLIVLNNAAAESAAGH"
     gene            1178..>1774
                     /locus_tag="VGA15_08940"
     CDS             1178..>1774
                     /locus_tag="VGA15_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587858.1"
                     /translation="MRELLFFIVSSFWAGAAHAATPGHGKTIAAAYIVGARGKPIDAV
                     ILGIFVTLSHTSGIVLVGVLASLGSTWLRPQRIEVYLAVAVGILVIVLGLWMLWAQRD
                     LVALAMGEAGEPGDGTSRAEAHARDHGHEQAHDHPHGHGHGEPVVWHSHGFGKVHAHR
                     LDVVAENRPKLPVLLALGIAGGLLPDPAALALLLGALSS"
BASE COUNT          334 a          560 c          577 g          303 t
ORIGIN      
        1 acccgctcga cgcgggattt gccgcggtgg tcgatccttc ggtctacatg cggtcgatca
       61 agatcatgat cgacgatccc gacaccgaca tcgtcatcat cgacgccgaa ctgccgaagg
      121 cgccgcacga attgcgcgaa aggaatttgc ggatcgtcga cgggatggca gccgcggcga
      181 gcaagcccgt ggtttacatc agcacaatgt cgatcgggtt caccgaattc accaagggct
      241 tgcgcaagtc gctgccgcat atcgccgtca tgcagggcct ggatcgcgcg gtgggcgcga
      301 tcaagtcgtt gatcgattac gcatccttgc gcaaggaagt gcccgaactc gtctcgagct
      361 ccagcgcgtc ggcgcgcgcg atgctggaga agatactgaa caacgcaagg ggcgcggcac
      421 tcgatgaggt cgcgtcgaaa aagctgctca aggcctatgg cattccggtc tccagggaag
      481 agatagccga gaccgcggca gacgccgtga agatcgccaa gaagatcggc tttcccgttg
      541 tcgccaaggt tgtcagcgcg gatatcctgc acaaatccga tattggcggc gtggtgctca
      601 acatcaacag cgccgccgag gtcaagaaag ccttcaacga catcaccgcg cgggtgaaga
      661 agctcaaggg caaaccgaaa ctcgagggca tcctgatcgc gcagcaggtc aaggccgagc
      721 tcgagctcgt ggtcggcgca tcgctcgatg ccgagatggg tccggtggtg ctgttcggca
      781 ccggcggcgt cgatatcgaa ctcatgaagg atgtcgcttt ggccggggct ccgctcgacg
      841 aggccgacgc cagggaattg atcaaccgga ccaaagccgg cgtgaaactg aagggctatc
      901 gcggcaagcc tgcgctgcac gaggcgtctg cgataaaggc gatcgtcggc ctctccaacc
      961 tgatggcaga cgccggcacc cgcatcgctt cgatcgacgt caatccattc ctgatcaata
     1021 ccaggaccgg cgttgccgtc gatggcctga tcgttctcaa caacgccgcc gccgaaagcg
     1081 ccgccgggca ttgatggtga aacgccataa ccagagccga cgtgctactt gtccacgcct
     1141 agcaacggcc ttggccggcc aactgatggt tgcctgaatg cgggaactcc tgttcttcat
     1201 cgtgtcgagc ttctgggcgg gcgccgcgca cgcggcaacg ccggggcacg gcaagacgat
     1261 cgcggccgcc tacatcgttg gcgcgcgcgg caagccgatc gacgcggtga tcctcggcat
     1321 tttcgtcacg ctgtcccaca ccagcggcat tgtgctggtc ggcgtgctcg cctcgctcgg
     1381 ctcgacctgg ctgcggccgc agcgcatcga agtctacctg gcggtcgcgg tcggcatcct
     1441 ggtgatcgtg ctcggcctat ggatgctgtg ggcgcagcgc gatcttgtgg cgctcgccat
     1501 gggcgaagcc ggcgagccag gcgatggcac cagtcgggcg gaagcccacg cgcgggatca
     1561 cggtcacgag caggctcacg accacccgca tggccatggt cacggcgagc ctgtggtctg
     1621 gcacagccac ggcttcggca aggtccacgc ccaccggctg gacgtcgtgg ccgagaaccg
     1681 tccgaaactg ccagtgctgc tggcgctggg tattgcgggc ggcctgctcc ccgatccagc
     1741 cgcgctggcg ctgctgctcg gcgcgctgtc gagc
//
LOCUS       DASWGM010000310         4191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_158549,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000310 DASWGM010000000
VERSION     DASWGM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158549"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGA15_08945"
     CDS             complement(<1..593)
                     /locus_tag="VGA15_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9587859.1"
                     /translation="MTATDAPIAGQSPPDSRRRDDPAEVPALSIDGVSHSYGARRALV
                     DISFTVAPASFTALLGLNGAGKSTLFSLVTRLFGIQAGRIGIFGHDIGLAPGEALRLL
                     GVVFQPRTLDLDLSVMQNLLYHAALHGIDRREARLRAGEVLTRIDLADRAGSKVRDLS
                     GGQMRRLEIARALLHRPRLLLLDEATVGLDVKARADIL"
     gene            complement(590..1576)
                     /locus_tag="VGA15_08950"
     CDS             complement(590..1576)
                     /locus_tag="VGA15_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HEX9587860.1"
                     /translation="MERLRMWHRCLFAGTAVWLAAATPARAVMAYVSNEKSNTVSVID
                     TDTWAVTKTIKVGQRPRGIEFTRDGKFVMVAVGDDDTIQVIDAATQQVVDTLPSGPDP
                     ELFVQDAAGKVLYVANENDNTVTIIDLEKRVRVGDVQVGVEPEGMAISPDGKILINTS
                     ETTNMAHFIDTATRQIVGNVLVDSRPRFAEFKRDGSELWVSSEIGGTVSVIDPAKHEV
                     TGKITFSVPGLRREAIQPVGIGMTQDGKTAFIALGPANRIAIVDAASHQVTKYLLVGQ
                     RVWHMAFTPDEKYLLVTNGVSNDVSIIDVVAQKVIKTIQVGELPWGITIAQP"
     gene            complement(1579..2763)
                     /locus_tag="VGA15_08955"
     CDS             complement(1579..2763)
                     /locus_tag="VGA15_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587861.1"
                     /translation="MIRLLAGAIGLGVLAGSALAADPVEIGIGYLHRGGVKSTLSLVE
                     QPADNDGLAGARLAIEDNNTTGKFLNQHFTLEEVRLKDSDDAAKAAVAFADRNINFII
                     ADLPADALLKAADAVRDRGTVLFNAGSIDDRLREEDCRANVIHAAPTRSMLADGLAQY
                     LVWKQWKRWLLVVGSHQEDKLFAEALRRAASRFGAKIVQERVFEDTGGARRTDSGVTL
                     IQRQMPVFTQQAPTYDVLVAADESEVFAAYLPYRTWDPRPVAGSAGLVPKSWDAAQDQ
                     WGAVQMQNRFLKLNSRHMTALDMQAWTAARMIGEAAARTNSANPKSLLDFLKGPDFSV
                     AAFKGQRLTLRDWNLQLRQPILLADGRMVVSVSPQEGFLHQVSELDTLGVDRPETKCK
                     LH"
     gene            2932..3411
                     /locus_tag="VGA15_08960"
     CDS             2932..3411
                     /locus_tag="VGA15_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HEX9587862.1"
                     /translation="MASVPARADPPKVAVFDFELVDTSLQGEVDGPRTDEQRRLMSTG
                     DQLRKALAESGKFAVLDIAPVNAAAHGSNLQSCGGCDVDYAQQLGADFAITGVVQKVS
                     NLILNMNVYIRDTRAGQLVTSMSADFRGNTDESWSRAASYLLRNRLLAPNYGAAQPQ"
     gene            complement(3428..>4191)
                     /gene="fghA"
                     /locus_tag="VGA15_08965"
     CDS             complement(3428..>4191)
                     /gene="fghA"
                     /locus_tag="VGA15_08965"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX9587863.1"
                     /translation="GMTFSVYVPPHPDGAKLPVVWYLSGLTCTHANVTEKGEFRRACS
                     ELGLIFVAPDTSPRGEGVPGDPAGAYDFGLGAGFYVDATQAPFARNYRMWSYVTEELP
                     ELIAEHFPADSKRQSILGHSMGGHGALTIALRHPDRYRAASAFSPIVAPSQVPWGIKA
                     LRGYLGDNREAWRKHDTVALIEDGARFSDFLVDCGDADPFLVEQLRPELLEHACAKAE
                     IPLVLRLHSGYDHSYYFVSTFMEDHLRWHAARLQT"
BASE COUNT          783 a         1297 c         1341 g          770 t
ORIGIN      
        1 aggatatcgg cgcgggcctt gacgtcgagg ccgacggttg cctcgtcgag cagcagcagg
       61 cgagggcgat gcagcagtgc gcgggcgatt tccagccgtc gcatctggcc gcccgagagg
      121 tcgcgcacct tgctgccggc gcggtcggca aggtcgatgc gcgtgagcac ttcaccggcg
      181 cgcaagcgtg cctcgcgccg gtcgatgccg tgcagggcgg catgatacag caggttttgc
      241 atgaccgaga gatcgaggtc gagcgtgcgc ggctggaaca ccacgcccag cagccgcaag
      301 gcttcgccgg gcgcaaggcc gatgtcgtgg ccgaagatgc cgatgcgtcc ggcctggatg
      361 ccgaacagcc gcgtgaccag cgagaacaat gtgcttttac ccgcaccgtt gaggccgagc
      421 agcgccgtga agcttgccgg cgcgacggtg aagctgatgt cgacgagcgc gcgtcgggca
      481 ccataggaat ggctgacgcc gtcgatcgac agcgccggca cttcggccgg atcgtcgcgg
      541 cggcgactgt ccggcgggct ttggccggcg atgggagcgt cggttgccgt catggctgcg
      601 caatcgtgat gccccagggc agctcgccga cctggatggt cttgatcacc ttttgcgcga
      661 ccacgtcgat gatcgagaca tcgttggata cgccgttggt caccagcaga tatttttcat
      721 ccggggtgaa cgccatgtgc cagacccgct ggccgaccag aaggtatttg gtgacctgat
      781 ggctggcggc atcgacgatc gcgatccggt tggccgggcc gagcgcgatg aaagcggtct
      841 tgccgtcttg ggtcatgccg atgccgaccg gctggatcgc ctcgcgccgc aggcccggga
      901 cgctgaatgt aatcttgccg gtaacctcgt gttttgccgg gtcgatgacg gacaccgtgc
      961 cgccgatctc cgaggacacc cacagttcgg agccgtcgcg cttgaattcg gcaaagcgcg
     1021 gtcgcgaatc gaccagcacg ttgccgacaa tctggcgcgt cgcggtatcg atgaaatgcg
     1081 ccatgttggt ggtctctgac gtattgatca gaatcttgcc gtccgggctg atggccatgc
     1141 cctcgggctc gacgccgacc tggacgtcgc cgacccgcac gcgcttttcg agatcgatga
     1201 tggtgaccgt gttgtcgttc tcgttggcga cataaagcac cttgccggcg gcatcctgca
     1261 cgaacagttc gggatcggga ccggagggca gggtgtccac cacctgctgg gtcgcagcgt
     1321 cgatcacctg aatggtgtcg tcatcgccga ccgcgaccat cacgaacttg ccgtccctgg
     1381 taaactcgat gccacgcggc cgctggccga ccttgatggt cttggtgacg gcccatgtgt
     1441 cggtatcgat caccgagacc gtgttgctct tctcgttcga cacataggcc atgaccgccc
     1501 tcgcgggcgt ggcggccgca agccagacgg ccgttccagc aaagagacaa cgatgccaca
     1561 tccgcaatct ctccatcttc agtgcagctt gcatttggtt tcggggcgat cgacgccgag
     1621 cgtatcgagt tccgagacct ggtgcaggaa accttcctgc ggcgagaccg aaaccaccat
     1681 gcgtccgtcc gccagcagga tcggctgacg caactgcagg ttccagtccc gcagcgtcag
     1741 ccgctggccc ttgaaggcgg cgacggaaaa atccggcccc ttgagaaagt cgagcaacga
     1801 ttttggattc gcggaattgg tgcgcgccgc cgcctcgccg atcatgcgcg ccgcggtcca
     1861 tgcctgcatg tcgagcgccg tcatatgccg cgaattcaac ttcagaaagc ggttttgcat
     1921 ctgcaccgcg ccccattgat cctgggcggc gtcccagctt ttcggaacaa ggccagcgga
     1981 tccggccacc ggccgcgggt cccaggttcg gtagggcaga tacgccgcaa acacctcgct
     2041 ctcatcggcc gcgacgagaa catcataggt gggggcctgc tgggtgaaca cgggcatttg
     2101 acgctggatc agggtcacgc cgctatcggt gcggcgagcg ccgccggtat cctcgaatac
     2161 ccgctcctgg acgatcttcg cgccaaagcg cgaggcggcg cgtcgcaacg cttcggcaaa
     2221 cagcttatcc tcttgatgcg atccgacgac cagcagccag cgcttccact gcttccagac
     2281 aagatattgc gcgagaccat ccgccagcat cgagcgcgtg ggggccgcat ggatcacgtt
     2341 ggcgcggcaa tcctcttcgc gcaagcgatc gtcgatcgag ccggcgttaa acaacacggt
     2401 tccgcggtcg cggaccgcgt cggcggcctt cagcagtgcg tccgcgggga gatcggcaat
     2461 gatgaaattg atgttgcgat cggcgaacgc aaccgccgcc ttggctgcgt cgtcgctgtc
     2521 cttcagccga acctcttcca gggtgaagtg ttgattgagg aatttgccgg tggtattgtt
     2581 gtcctcgatc gcgaggcggg cgcccgccag gccatcattg tcggccggtt gctcgaccag
     2641 cgacagtgtc gacttgacac cgccgcgatg aagatagccg atgccgattt cgaccggatc
     2701 ggccgcaagc gcactgccag ccagcacacc caagccgatc gcgccggcca acaatcggat
     2761 catgtttcct ccctgacatt cctcctcgac gggaccgacg atggagggac ttccggcttg
     2821 aaccatggtc aaattgtcct acgatgcaac cgacattttg tcgccgcgcc gccgcggatt
     2881 tcgcggatcc cgatcatgaa atatctttcg gccctcgtcg cggtgatgct gatggcgtca
     2941 gtgccggcgc gcgccgaccc acccaaagtc gcggtgtttg atttcgaact ggtggacacc
     3001 agccttcagg gcgaagtgga tgggccgcgg accgacgaac aacggcggct gatgagcacc
     3061 ggcgatcagc tgcgcaaggc attggcggaa tccggaaagt ttgcggtcct cgacatcgcg
     3121 ccggtcaatg ctgcggcgca cggcagcaat ctgcagtcct gcggcggctg cgacgtggac
     3181 tacgcacaac agctcggtgc cgatttcgcc atcacgggtg tggttcaaaa ggtctcgaac
     3241 cttattctga atatgaatgt ctacatacgg gacacccgag ccggacagct cgtcacgtcg
     3301 atgagcgccg actttcgcgg caataccgac gaatcctggt cccgcgctgc gagctatctg
     3361 ctgcgaaatc ggctgctcgc gcccaactac ggcgcggcgc aaccgcaata gcggttcggg
     3421 cgggcgctta agtttgcaac cgcgcggcgt gccaacgcag atgatcttcc atgaaggtgg
     3481 aaacgaagta gtagctgtgg tcgtagccgc tgtggagacg aagcacgagg gggatttcag
     3541 ccttcgcaca tgcgtgctcg agcagttccg ggcgaagctg ttccaccagg aaggggtcgg
     3601 catcgccgca atcgacaaga aaatcggaaa accgggcgcc gtcctcgatc agcgcgacgg
     3661 tgtcatgctt gcgccacgct tcccggttat cgccgagata acctcgcaac gccttgatgc
     3721 cccagggtac ttgcgagggt gccacgattg gcgaaaaagc gctggcggcg cggtaacgat
     3781 cgggatgtcg cagcgctatg gtcagcgcgc cgtggccgcc catcgaatga ccgagaatgg
     3841 attgccgttt ggaatcggcg ggaaaatgct cggcgatcaa ttcgggcaat tcttcggtca
     3901 catagctcca catccggtaa ttgcgggcaa acggcgcttg cgtggcatcg acatagaagc
     3961 cggcgccaag cccgaaatca taggcgccgg cgggatcgcc gggcacgcct tcgccgcgcg
     4021 gactggtgtc cggcgcgaca aagatcaatc ccagttcgga gcaggccctt cgaaattcac
     4081 ccttttccgt gacgttggcg tgggtgcagg tcagacccga taaataccag accaccggca
     4141 gcttcgcgcc atccggatgc ggagggacat agaccgaaaa ggtcatgccg g
//
LOCUS       DASWGM010000311         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1829298,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000311 DASWGM010000000
VERSION     DASWGM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829298"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2811
                     /locus_tag="VGA15_08970"
     CDS             <1..2811
                     /locus_tag="VGA15_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NahK/ErcS family hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HEX9587864.1"
                     /translation="RAIEYVPSIGNFLTMTLLSFCAIMLLPRQFHVSVVENSSDAEVG
                     RARWLFPLYLVAINLFVIPIALAGLVTFPFGAVDSDMFVLALPMAGDSPWLSVGVFVG
                     GLSAATAMVIVECVALSIMVSNDIVVPLVLQRGAQTRAGQKDFGDFLLRVRRFAIFAI
                     MAMAYFYYRALGNTQLVAIGLLSFAAIAQLAPAFFGALFWRRATARGAMGGMLVGFAV
                     WIYTLFLPSFLDGNTAGMMLLQHGPFGIEALRPQALFGADLPPLLHGVLWSLALNCVT
                     YVVLSLARRPSSIERLQADLFVPNALAPIAPTFLRWRTTVTVQDIQSTVAQYLGPDRA
                     RHSFEAFAITRHASLEPSAPADFELLQHAEHLIASSIGAASSRLVMSLLLRKRTVSAK
                     AALKLLDDSHAALHFNREILQTALNHVRQGIAVFDADSQLICSNRQFGEILGLPPHLV
                     QIGIPLREILEFMGAVNPLAFGDGDALLEKRLTAYTTEGEPYLERLPDRHMVIEVRAN
                     RMPGGGLVITFFDVTPSFEAAEALERSNATLEKRVRDRTEELTRLNSELAQAKSTAED
                     ANISKTRFLAAASHDILQPLNAARLYVTSLVERQNGGEDSRLVENIDDSLEAIEEILG
                     ALLDISRLDAGAMTTSITSFKIGDLMRSLEIEFAPMARAKALKLTFVPCSLPVESDRS
                     LLRRLLQNLISNAIKYTPRGRVLVGCRRRGESLQIGVYDTGVGIPVQKRGEIFKEFHR
                     LEQGARIARGLGLGLSIVERLGRVLNHSIAIDSNASGGSVFSVTVPIASAINHTTAVT
                     SATPLSRTPMSGSLIVCIENDPAILDGMKTLLTAWDAEVIAVADPDAAIEAIEAAGGR
                     VTGLLVDYHLDRGNGVAAIRDIRRRFGDTIPAILITADRSPHVRAAAREEQITILNKP
                     VKPASLRALLGQWRTQQMVAAE"
     gene            complement(2844..4142)
                     /locus_tag="VGA15_08975"
     CDS             complement(2844..4142)
                     /locus_tag="VGA15_08975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9587865.1"
                     /translation="MSRPPTVLSRRAALATLAAAAYASATAGSMVEGMAAENELGPVF
                     SPTGPDAELYGAAEGFPIADRSLPIQPGNPYQPKYRVGAFSHFDEIYPTRRIKRAATP
                     WMFKRSQAEFSYSYLGDRSSLAEYLSRNPVTGLLIAKDDQIVFEHYQYGRTDRDRLIS
                     QSMAKSITGMLIGIAISEGAIKSVDDTPETYVPGFKGTEYGRTPIRDLLHLSSGVEFG
                     EDEDNGRDLNRLWTNMVLGLGSPTGTVNSIAQFNRRIAPPGTRYAYASIEPDVLGVVL
                     HQAVNKSASDYLQEKVWQPIGAEADATWLLDAEGFELAHFGFNAVLRDYARLGRLLAH
                     DGAWQGNQIIPAQWMMDATTVRASDAYLLPGKAMPTFGYGYLLWLLPGTRRQFALVGD
                     LGQRICVDPASKLVMVQTALETRAEVWRLWSAVVEQLGQR"
     gene            complement(4349..5014)
                     /locus_tag="VGA15_08980"
     CDS             complement(4349..5014)
                     /locus_tag="VGA15_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9587866.1"
                     /translation="MTSAASTHLVIADDHPLFRDALRQAVASVVTSARIDEAGSFDEL
                     TALLEQDSDVDLILLDLTMPGISGFSGLIYLRAQYPAIPVVIVSASDDGGTIRRSLDF
                     GASGFIPKRFGVETLRDAIVKIMDGDVWVPPDTDLTSAADPDMSRLRDRLVTLTPQQV
                     RVLMMLSEGLLNKQIAYELGVSEATIKAHVSAILQKLGVESRTQAVIAAAKIAGGQWR
                     PSA"
     gene            5370..>5767
                     /locus_tag="VGA15_08985"
     CDS             5370..>5767
                     /locus_tag="VGA15_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587867.1"
                     /translation="MSTLAATEVRTGGMTKDERFVILASSLGTVFEWYDFYLYGSLAS
                     IIGAQFFSAYPPATRDIFALLAFASGFLVRPFGAIVFGRVGDIVGRKYTFLVTILIMG
                     LSTFIVGLLPNAATIGFAAPVILIGLRLAQG"
BASE COUNT          993 a         1822 c         1816 g         1136 t
ORIGIN      
        1 cgcgccatcg agtatgtgcc ctcgatcggc aatttcctca ccatgacgct gctgtcgttt
       61 tgcgcgatca tgctgctgcc gcgccagttc cacgtcagcg tggtggagaa ttccagcgat
      121 gccgaggtcg gccgggcgcg ctggctgttt ccgctgtatc tggtcgccat caacctgttc
      181 gtgattccga tcgcgcttgc aggtctggtc acctttccgt tcggcgcggt cgacagcgac
      241 atgtttgtgc tggcgttgcc gatggcgggc gattcgccgt ggctcagcgt cggggtgttt
      301 gtcggcggcc tctcggcggc gaccgccatg gtgatcgtgg aatgcgtcgc gctgtcgatc
      361 atggtttcca acgacatcgt ggtgccgctg gtgctgcagc ggggcgcgca aacgcgcgcg
      421 ggccagaagg attttggcga cttcctgctc agggtccgcc ggtttgcgat ttttgccatc
      481 atggcgatgg cctatttcta ctaccgcgcg ctgggcaaca cccaactggt ggcgatcggc
      541 ctgttgtcct ttgcggccat cgcgcagctt gcgccggcat tcttcggtgc cctgttctgg
      601 cggcgcgcga ccgcgcgcgg cgccatgggc ggcatgctgg tcgggttcgc ggtgtggatc
      661 tatacgctgt ttctgccgag ctttctggac ggcaataccg ccggcatgat gctgctgcag
      721 cacggcccgt tcggcatcga ggcgttgcgc ccgcaggccc tgttcggcgc cgatttgccg
      781 ccgctgctgc acggcgtgct ctggagcctc gcgcttaact gtgtgaccta cgtcgtgctg
      841 tcgctggcgc gccggccgtc ctcgatcgaa cggttgcagg cggatctgtt cgtccccaat
      901 gcgctggcgc cgattgcgcc gaccttcctg cgctggcgta ccacggtaac cgtgcaggac
      961 attcagagca cggtggcgca gtatctcggt cccgatcgcg cccgccattc gttcgaagct
     1021 tttgccatta cccgccacgc cagcctcgaa ccgtcggcgc cggccgactt cgagctgctg
     1081 cagcatgccg agcatctgat cgcttcctcg attggcgccg cgtcctcgcg cctggtaatg
     1141 tcgctattgc tgcgcaagcg taccgtctcg gccaaggccg ccctcaaact gctggacgat
     1201 tcccacgccg cattgcattt caatcgcgag atattgcaga ccgcgctcaa ccatgtgcgg
     1261 cagggcatcg cggtgttcga cgcggattcg cagctgatct gttcgaaccg gcagttcggc
     1321 gaaattcttg ggctgccgcc gcacctcgtg cagatcggga ttccgctgcg cgaaatcctc
     1381 gaattcatgg gagcggtcaa tccgctcgcc ttcggcgacg gcgacgcttt actggagaag
     1441 cggctgaccg cctataccac cgaaggcgag ccgtatctgg aacgcttgcc ggatcgtcac
     1501 atggtgatcg aggtgcgcgc gaaccggatg cccggcggcg gactggtcat cacgtttttc
     1561 gacgtcacgc catcctttga agcggccgaa gcgctggagc gctccaacgc aaccctggaa
     1621 aaacgcgtgc gcgatcgcac cgaggagctc acccgcctga actcggaact ggcgcaggcc
     1681 aagagcaccg cggaggacgc caacatctcg aaaacgcggt ttctggcggc ggcaagccac
     1741 gacatcctgc aaccgctgaa cgcggcgcgg ctctatgtga cgagcctcgt cgagcgccag
     1801 aacggcggcg aggattcgcg gctggtcgaa aacatcgacg attcgctgga ggcgatcgag
     1861 gaaatcctcg gcgcgctgct ggatatttcg cggctcgacg ccggcgccat gacgacctcg
     1921 atcaccagtt tcaagatcgg cgacctgatg cgttcgctgg agatcgaatt cgcgccgatg
     1981 gcgcgcgcca aggcgctgaa gctgacattc gtgccgtgtt cgttgccggt ggaatccgat
     2041 cgctcgttgc tgcggcggct gctgcagaac ctgatctcca acgccatcaa atataccccg
     2101 cgcgggcgcg tgctggtcgg ttgccggcgc cgcggcgaat cgctgcaaat cggcgtttat
     2161 gataccggcg tcggcattcc cgtgcagaag cgcggcgaga tcttcaagga gtttcaccgg
     2221 ctggaacagg gcgcccggat tgcccgtggt ctcgggcttg ggctgtcgat cgtcgagcgc
     2281 ctcggccgcg tcctcaacca cagcatcgcg atcgattcca atgccagcgg cggttcggtg
     2341 ttttcggtca cggtgcccat cgccagcgcc atcaatcaca ccacagccgt caccagcgcc
     2401 accccgcttt cgaggacgcc gatgagcggc tccctgatcg tgtgtatcga aaacgatccg
     2461 gccatcctgg acggcatgaa gacgctgctg acggcctggg atgccgaggt catcgcggtc
     2521 gccgatccgg atgccgccat cgaggcgatc gaggccgcgg gcgggcgggt caccggtctt
     2581 ctggtcgact atcatctcga tcgcggcaac ggcgtcgccg cgatccgcga cattcgccgc
     2641 cggttcggcg acaccattcc ggcgatcctg atcaccgcgg atcgcagtcc gcatgtgcgg
     2701 gctgccgccc gggaggagca aatcacgatc ctcaacaaac cggtcaagcc ggcatcgttg
     2761 cgggccctgc tcggccaatg gcgcacccag cagatggtgg ccgcggaata gctgcggaag
     2821 tcaggatgat cgcaggcgcg gcgtcaccgc tggccgagtt gttccaccac ggccgaccat
     2881 agccgccaga cctcggccct ggtctcgagc gcggtctgga ccatcacaag ctttgaggcc
     2941 gggtcgacgc aaatgcgctg gccgaggtcg ccgacgagcg cgaactggcg ccgcgtaccc
     3001 ggaagcagcc acaacagata gccatagccg aacgttggca tcgccttgcc cggcagcaag
     3061 taggcgtcgg aggcgcgcac cgtcgtggcg tccatcatcc attgcgccgg gatgatctgg
     3121 ttgccttgcc atgcgccgtc atgggcaagc aggcgcccga gccgcgcgta gtcgcgcagc
     3181 acggcgttga agccgaaatg cgccagttcg aacccttccg catccaaaag ccaggtggcg
     3241 tcggcttccg cgccgatcgg ctgccacact ttttcctgca ggtagtcgga cgccgattta
     3301 ttgacggctt ggtgcagcac cacgccgagc acgtcgggtt cgatgctggc gtaagcgtac
     3361 ctcgttcccg gcggcgcgat ccgccggttg aattgcgcga tgctgttgac cgtgccggtc
     3421 ggggacccca atcccagcac catgttggtc cacaagcgat tgaggtcgcg tccgttgtct
     3481 tcgtcttcgc cgaattcaac gcccgaggat aaatgaagca ggtcgcggat cggcgtcctg
     3541 ccatattccg tgcccttgaa tcccggcaca taggtctccg gcgtgtcgtc gaccgatttg
     3601 atcgcgcctt ccgaaatggc aatgccgatc aacatccccg tgatcgattt agccatcgac
     3661 tgcgaaatga gccggtcgcg atcggtacgg ccgtattgat aatgctcgaa gacaatctgg
     3721 tcatccttgg cgatcagcag accggtgacg ggattgcgcg acagatattc cgccagcgaa
     3781 gatcgatctc ccagatacga atagctgaat tcggcttgcg aacgtttgaa catccatggc
     3841 gtcgcggcgc gcttgatccg ccgcgtcgga tagatttcat cgaaatggct gaacgcgccg
     3901 acacgatatt tgggctgata cgggttgccg ggttgaatcg ggagcgagcg atcggcgatc
     3961 ggaaagcctt ccgcagctcc ataaagctcg gcgtcgggac cggtgggcga aaacaccgga
     4021 ccaagctcgt tttcggccgc catgccctca accatcgatc cggccgtggc ggaggcatat
     4081 gcggcggcgg ccagcgttgc gagcgccgcg cggcgagaca agacggttgg cggcctcgac
     4141 atgcgattcc ccctgttgtt gcagcaaggc aagtcgcagt cgctcatacg ccttctgcag
     4201 atccgatgtc gagtcagggg gcgataccgg ttgcagcacc gaacggacgc cgcggcggat
     4261 agtcggaatc ggacgcaccg ccgagcgatc gcggcggcgg aagccttttt cggccgatat
     4321 ctttcgggct ctcagtgagt tgcggacgtc acgcgctggg ccgccactgg ccgccggcga
     4381 ttttcgccgc ggcgatcacc gcctgggtgc ggctctcgac gccgagcttc tgcaggatcg
     4441 ccgagacatg cgccttgatg gtcgcttccg acacaccaag ctcgtaggcg atctgcttgt
     4501 tgagcaggcc ttccgacagc atcatcagca ccctcacctg ctgcggggtc agcgtcacaa
     4561 ggcggtcgcg caggcggctc atatccggat cggccgcgga ggtgaggtcg gtatccggcg
     4621 gaacccagac gtcgccatcc atgattttca cgatcgcgtc gcgcagcgtt tcgaccccga
     4681 accgcttggg aatgaacccc gacgcgccga aatccagcga gcggcggatg gtgccgccgt
     4741 cgtcgctggc cgaaacgatg accacgggga tcgccggata ttgcgcgcgc aggtaaatca
     4801 ggccggagaa accgctgatg ccgggcatcg tcagatcgag caggatcagg tcgacatcgg
     4861 aatcctgctc aagcagcgcg gtcagctcat cgaacgaacc ggcttcgtca atcctggccg
     4921 atgtcacgac actggcaacc gcctgtcgca aggcatcgcg gaacagcgga tggtcatcgg
     4981 caatgacgag atgcgtgctg gcggcggaag tcatttatct cctgcaaaag ggttgcggca
     5041 aagccgtgat cgcgtgtcgt agagggcgcg ccaagcgaat tccttgtcgc aattgtcctc
     5101 tgagcgacgc ttgcatgcaa gcatccaata ttgctatgcc gcaaagcggt taatcgcccg
     5161 gtttgtgcgc cgcaatacgc cggttcccga tggcatgatc ggggttgttc gaggcgcgga
     5221 ctcctcggca tgccgcgcaa agggccaaaa gtcttattgg gggaggtttc acggcgatgt
     5281 taccgtgaac cgaagcctgt agctgtaatc cggcccagtt cggcgcatca ggcgatcttt
     5341 gcatcaacga tttcggggag cgatccaaaa tgtccacatt ggctgcaacg gaagtcagaa
     5401 ccggaggcat gacgaaggac gaacgtttcg tcattctcgc gtcatcgctc ggcaccgttt
     5461 tcgaatggta tgatttctat ctttacggat cgctcgcaag catcatcggc gcgcaattct
     5521 tctcggccta tccgccggcg acgcgcgata ttttcgcgct gctcgccttc gcctccggat
     5581 tcctggtgcg cccgttcggc gccatcgtgt tcgggcgcgt cggcgacatc gtcggacgca
     5641 aatacacctt cctcgtcacc atcctgatca tgggtctgtc gaccttcatc gtcggcctgt
     5701 tgcctaacgc ggcaaccatc ggctttgccg cgccggtcat tctgatcggg ttgcgtctgg
     5761 cgcaagg
//
LOCUS       DASWGM010000312         6599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_575177,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000312 DASWGM010000000
VERSION     DASWGM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575177"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VGA15_08990"
     CDS             complement(<1..1055)
                     /locus_tag="VGA15_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587868.1"
                     /translation="MPPVLNIIALATFAASLSARALDPVLPHVASDFNVSIAAAAGFA
                     SVFAFTFAIIQPVLGAAADLFGKARLVILCLVLLGLANILGALSSSFSLLFATRILAG
                     IGAGGVFPIALGLTSDLIAPDKRQVAIGRTLAGAMTGNLLGASLSGLIGDFLGWRGVL
                     AVLGSLAVVASVAVAVGFRGGALRRAPAKVDLSVLRHGYRTIFANPNARVCYTAVFIE
                     GCCVLGLFPYVASFLFEQGVTSLSISGLVIAGFAVGGLFYTLSVSRFLPRFGVNGMMM
                     SGAVLMGLQLAIVAFGPPWQLQAGNFILMGWGFYMIHGSLQVFASELSVEARATALSL
                     HSFFFFMGQTIGPIAYG"
     gene            complement(1092..1943)
                     /gene="kdsA"
                     /locus_tag="VGA15_08995"
     CDS             complement(1092..1943)
                     /gene="kdsA"
                     /locus_tag="VGA15_08995"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX9587869.1"
                     /translation="MNANISAAPVVTAGHVKFGNELPISIIAGPCQLESRAHALEVAG
                     ALKEIATRLKIGLIYKTSFDKANRTSVSAARGIGLAQALPIFAEIRSSLGMPVLTDVH
                     EAAQCAEVAQAVDVLQIPAFLCRQTDLLLAAAATGKVVNVKKGQFLAPWDMANVVAKI
                     TGAGNPNVLVTERGASFGYNTLVSDMRALPILARTTGAPVIFDATHSVQQPGGKGTSS
                     GGEREFVPVLARAAVAVGVAGVFIETHPDPDRAPSDGPNMVPLREFEGLVKTLMAFDA
                     LAKSMAR"
     gene            complement(2001..2135)
                     /locus_tag="VGA15_09000"
                     /pseudo
     CDS             complement(2001..2135)
                     /locus_tag="VGA15_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(2167..2493)
                     /locus_tag="VGA15_09005"
     CDS             complement(2167..2493)
                     /locus_tag="VGA15_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX9587870.1"
                     /translation="MIYETRVYRCLPGRLPALLKRFETITLKLWEKHGIKQAGFFTTL
                     VGESNQELTYLLAWESLADREKKWAAFQSDPDWIAARAKTEEDGQIVGNVVNQLLVPT
                     SFSSVK"
     gene            2617..2871
                     /locus_tag="VGA15_09010"
     CDS             2617..2871
                     /locus_tag="VGA15_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX9587871.1"
                     /translation="MVDFDPAKEAINLSKHGVSLARWVDLDIAVAFVDDRYDYGEVRY
                     RAYGYIDGVAYCLAFTSRNGKVRPISLRRVHTKEMKRYVS"
     gene            2861..3133
                     /locus_tag="VGA15_09015"
     CDS             2861..3133
                     /locus_tag="VGA15_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX9587872.1"
                     /translation="MSRKLKEPIFDDDNPEWTKADFAKARPPAEVLPPELLAQFKNTR
                     GPQKTPTKVAVSIRLSPEVISHFKAKGPGWQSRIDDALRKIVKKAS"
     gene            complement(3294..4925)
                     /locus_tag="VGA15_09020"
     CDS             complement(3294..4925)
                     /locus_tag="VGA15_09020"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX9587873.1"
                     /translation="MARYIFITGGVVSSLGKGLASAALGALLQARGYKVRLRKLDPYL
                     NLDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGRPATKADNITTGRIYQDILTKE
                     RRGDYLGATIQVIPHVTNAIKDFILDSNDDYDFVLCEIGGTVGDIEGLPFFEAIRQIK
                     NDLPRDHAIYIHLTLLPFIPSAGELKTKPTQHSVKELRSIGIQPDILLCRTDRPIPKE
                     ERRKLGLFCNVRESAVIEARDVDNIYAVPEAYHAAGLDDEVLAAFGIEPKIPPDLQRW
                     HVINERVRNPEGAVTIAIVGKYTGMKDAYKSLIEALSHGGIANKVRVNLDWIESEVFE
                     NEDPAPFLEHVNGILVPGGFGQRGAEGKIRAAQFARERDVPYFGICFGMQMAVIEAAR
                     NLVGIEEANSTEFGPTPEPLVGLMTEWLRGNELEKRSKAGDLGGTMRLGAYPAVLKRG
                     SRVSEVYGGATEISERHRHRYEVNTTYKDRLEQHGLRFSGLSPDGVLPEIVEYEDHPW
                     FIGVQFHPELKSRPLEPHPLFASFVQAAVVQSRLV"
     gene            complement(5092..5490)
                     /gene="secG"
                     /locus_tag="VGA15_09025"
     CDS             complement(5092..5490)
                     /gene="secG"
                     /locus_tag="VGA15_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX9587874.1"
                     /translation="MQTVVIVIHLMIVATLIGAVLLQKSEGGGLGVGGGAGFMSSRGT
                     ANLLTRTTAVLAVGFFITSLFLSWIASYDRKPSSIIGGTPASQSQPAGPATPAPPLSQ
                     GGGILDSLKKADEQQQNQPAPSGPQAPRSQ"
     gene            5724..6287
                     /locus_tag="VGA15_09030"
     CDS             5724..6287
                     /locus_tag="VGA15_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX9587875.1"
                     /translation="MSLLLDDTSSRIAGRLRLERENRGWSLADLAERSGVSKATISKI
                     ERKEVSPTAVILVRLAGAFDLTLAGLLLRAEGQGRLTRATEQPVWRDPDTGYLRKQVF
                     GRPDHPVEIIRVELPAGQRVVLPASSYVHIRQAVWVQAGHLVIIEAGERRELGTGDCL
                     GFGPPSEVTFANETTAWCVYIVVLARS"
     gene            6293..>6599
                     /locus_tag="VGA15_09035"
     CDS             6293..>6599
                     /locus_tag="VGA15_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587876.1"
                     /translation="MSEIDIKPRATEPKSQASGLTAGHNGGPPLHEAHKPEWGEGPIG
                     NYFYWKAAHRTAWHSASRDVILFRIQRAERLGLTYEEYTLEILERGRHLQIEDVERIA
                     "
BASE COUNT         1320 a         2056 c         2044 g         1179 t
ORIGIN      
        1 aacccgtagg cgatcggtcc gatggtctgg cccatgaaga agaagaacga gtgcagcgac
       61 agcgcggtgg cgcgggcttc caccgaaagt tcgctggcaa acacctgcag cgagccgtgg
      121 atcatgtaga agccccaccc catcaggatg aagttgccgg cctgaagctg ccagggcggg
      181 ccgaaggcga cgatggcgag ctgcaggccc atcagtacgg cacccgacat catcatgccg
      241 ttgacgccga accgcggcag aaagcgcgac acgctcaagg tgtagaacag tccgccgaca
      301 gcgaagcctg cgatcacgag cccgctgatg gaaaggctgg tgacgccctg ttcgaacaga
      361 aacgacgcga catagggaaa caggccgagc acgcagcagc cttctatgaa taccgccgtg
      421 tagcagaccc gcgcattcgg attggcaaag atggtgcgat agccatgccg cagcaccgaa
      481 agatcgactt tcgccggtgc ccgcctcagt gcgccgccgc gaaagccgac ggccaccgcg
      541 accgacgcca cgacggcgag cgagccgagg acggcgagca caccgcgcca gccgaggaaa
      601 tcgccgatca ggcccgacag cgacgccccg agcagattgc cggtcatcgc tcccgccagc
      661 gtgcggccga tggcgacctg ccgtttgtcc ggcgcaatca gatcgctggt cagcccgagc
      721 gcgatcggaa atacgccgcc ggcgccgatc cccgccagta ttcgcgtcgc gaacaacagc
      781 gaaaacgacg acgacagcgc gccgagaatg ttggcgaggc cgagcagcac caggcacagg
      841 atcacgaggc gcgccttgcc gaacaaatcc gccgcggcgc ccagcaccgg ctggatgatc
      901 gcgaaggtga atgcaaacac cgacgcaaac cctgccgccg cggcaatgct gacattgaag
      961 tcgctggcga cgtgcggcag taccggatcg agcgcccgcg ccgacaggct cgcggcgaac
     1021 gtcgccagcg caatgatatt caggaccggc ggcatgtgca ggtcatgacg gccgcagccc
     1081 tgctcgcaac atcaacgcgc catgctcttg gcgagcgcgt cgaacgccat cagcgtcttc
     1141 accaaacctt cgaactcgcg cagcggcacc atgttggggc cgtccgacgg cgcgcggtcg
     1201 ggatcgggat gggtttcgat gaacacgccg gcgacgccga ccgcgaccgc ggcgcgcgcc
     1261 agcaccggca cgaattcacg ctcgccgccc gatgaagttc ccttgccgcc gggctgctgc
     1321 accgaatggg tggcgtcgaa gatgaccggc gcgccggtag tccgcgccag gatcggcagc
     1381 gcgcgcatat ccgacaccag cgtgttgtag ccgaacgatg cgccgcgctc ggtgaccagc
     1441 acgtttggat tgccggcgcc ggtgatcttg gcgacgacat tcgccatgtc ccacggcgcc
     1501 aggaattgcc ccttcttgac attgacgacc ttgccggtcg ccgccgccgc caacagcaga
     1561 tcggtctgcc ggcacaggaa cgccgggatc tgcagcacgt cgacggcctg cgccacttcg
     1621 gcgcattggg cggcctcatg aacgtcggtg agcacgggca tgcccagcga cgagcgtatc
     1681 tcggcaaaga tcggcagcgc ctgcgcaagt ccgatgccgc gcgcggcgga gacgctggtg
     1741 cggttggcct tgtcaaagga cgtcttgtag atcagcccga tcttcaagcg cgtcgcgatc
     1801 tccttcagcg cgccggcgac ttcgagcgca tgcgcccggc tttcgagctg acatggcccc
     1861 gcaatgatcg aaatcggcaa ctcattgccg aacttaacgt ggcccgcagt aacgaccggc
     1921 gcagctgaga tgttggcgtt caaggcacta tcctttttcc ggcgcgacca tgccgagcag
     1981 cgcccggggg atcaaccctc ttgcacaatg cggttgtggg ggccccatgc atggcggttg
     2041 cgtgcgccga cggtaaagcc ggcgctcgca tagaattccg cgaccgcgtc gagatcgcgg
     2101 acggcgtgca cgatatggtc gagaccgtgg ggcatcggcg gcgacctcaa cccttcgaaa
     2161 tgccggtcac ttcaccgacg agaacgaggt cggcaccagc aattgattga cgacgttgcc
     2221 gacgatctgg ccgtcttcct cggtcttggc gcgcgccgcg atccagtcgg ggtcgctctg
     2281 gaatgccgcc catttcttct cgcgatcggc gagcgattcc catgccagca gataggtcag
     2341 ttcctgattg gattcgccga ccagcgtggt gaagaacccg gcctgcttga tgccgtgctt
     2401 ctcccacaac tttagcgtga tggtctcgaa gcgcttcaag agcgccggca gccggcccgg
     2461 caggcagcga tagacgcggg tttcgtagat catggatttc ctccgttttc ttgcggaggc
     2521 gttatagcga ctggacggaa agttgtcttg aggggacgat cacatggcac ccctgcccca
     2581 gcttgacgca tttgcaaaca aattgtatat acaataatgg ttgactttga tccggccaag
     2641 gaagcgatca atctatcgaa gcatggcgta tcgctggcgc gctgggtcga tctcgatatc
     2701 gcggtggcct ttgtagacga ccgctatgac tacggcgagg tccgctatcg cgcttacggc
     2761 tacattgacg gcgtcgcata ttgcttggcc ttcaccagtc gaaatggaaa ggtgcgtccg
     2821 atcagccttc gacgcgtgca tacaaaggag atgaagcgct atgtctcgta aattgaaaga
     2881 gccgatattc gacgacgaca atccggaatg gaccaaagcg gatttcgcca aggcgagacc
     2941 tcccgcagag gtccttccgc cggaattgct tgctcagttc aagaacacgc gcgggccaca
     3001 gaagacgcca actaaggtcg cggtgtcgat caggctaagc cccgaggtca tttcgcattt
     3061 caaagccaaa ggtccgggct ggcaatcgcg gatcgacgac gccctgcgca aaatcgtcaa
     3121 gaaggcaagc tgatctgccg gctcaaacca accggccctg ccccaccgtc gcttggatga
     3181 aggaagcgaa cagcggatgc ggctcgaacg gccgcgattt cagttcggga tgaaattgca
     3241 cccccgacaa ccaaggtaaa caccgattca gctacagacg caacctccaa cggctaaacc
     3301 aaccggctct gcaccaccgc ggcctgaacg aacgacgcga acagcggatg cggttcgagc
     3361 gggcgcgatt tcagttcggg atggaattgc acgccgatga accacgggtg atcttcgtat
     3421 tcgacgattt ccggcagcac gccatcgggc gaaagacctg aaaaacgcag accatgctgt
     3481 tcgagccggt ccttgtaggt ggtgttgact tcgtaacgat ggcgatggcg ctccgaaatc
     3541 tcggtggcgc cgccataaac ctccgacacc cggctgccgc gcttgagcac cgcaggatat
     3601 gccccaaggc gcatggtgcc gccgagatcg ccggccttcg atcgcttctc cagctcgttg
     3661 ccgcgcagcc actccgtcat caggccgacc agaggttcgg gggtcgggcc gaactcggtg
     3721 gaattggctt cctcgatgcc gaccagattg cgcgcggcct cgatcaccgc catctgcatg
     3781 ccgaagcaga tcccgaaata cggcacgtcg cgctcgcgtg cgaactgcgc cgcgcggatt
     3841 tttccttcgg cgccgcgctg accaaaaccg ccgggcacca gaatgccgtt gacgtgctcg
     3901 agaaacggcg cgggatcctc gttctcgaac acttcactct cgatccagtc gagattgacc
     3961 ctgaccttgt tggcgatgcc gccgtgcgac aaggcctcga tcagcgattt gtaggcgtcc
     4021 ttcatgccgg tgtatttgcc gacgatggcg atggtcaccg caccctcggg attgcgcacc
     4081 cgctcgttga tgacgtgcca gcgctgcagg tcgggcggaa tcttgggttc gatgccgaac
     4141 gccgccagca cctcgtcgtc gaggccggcg gcgtgatacg cctcgggcac ggcgtagatg
     4201 ttgtcgacgt cgcgcgcctc gatcacagca ctttcgcgca cgttgcagaa caggccgagc
     4261 ttgcgccgct cttccttcgg gatcggccgg tcggtgcggc acagcaggat gtccggctgg
     4321 atgccgatcg agcgcagttc cttgaccgag tgctgggtcg gctttgtctt taattcgccg
     4381 gcactcggga tgaacggcaa cagcgtcaaa tgaatgtaga tggcgtggtc gcgcggcagg
     4441 tcgttcttga tctggcggat cgcctcgaag aacggcaacc cctcgatatc gccgacggtg
     4501 ccgccgatct cgcacagcac gaagtcgtaa tcgtcattac tgtcgagaat gaaatccttg
     4561 atggcattgg tgacatgcgg gatcacctgg atggtggcgc cgagataatc gccgcgccgt
     4621 tccttggtga ggatgtcctg gtagatgcgc ccggtggtga tgttgtcggc cttggtggcc
     4681 gggcgtcccg taaaacgctc gtagtgtccg agatcgagat cggtctcggc gccgtcgtcg
     4741 gtcacgaaca cttcgccgtg ctgatacggc gacatcgttc cgggatcgag attgagatag
     4801 ggatcgagct tgcgaagccg aaccttgtat ccgcgtgcct gcagcagggc gccgagcgcc
     4861 gctgaagcca gaccttttcc gagcgaggag accacgccgc cggtgatgaa gatataccgc
     4921 gccatgggac ccaaccttta aggctaccgg tgcgattcgc caaaacgaat cgtacccctc
     4981 agcgaacatt tcgtttggct gtgggtgacg tgaaattgaa aacaatatca ggatgttgat
     5041 ggaggctcct catgcacgac ggtaggcgtc gtcatgcatg gcccccctgc tttattgcga
     5101 acgtggcgct tgcggacccg atggcgccgg ctgattctgc tgctgctcgt ccgctttttt
     5161 cagcgaatcc agaatgcccc caccctgcga cagcggcggg gcgggcgtcg ccggacctgc
     5221 cggctgagac tgcgaggcgg gagtgccgcc aatgatcgag ctcggcttgc ggtcgtagct
     5281 ggcgatccag gacaggaaca ggctggtgat aaagaagccc accgcgagaa ccgccgtcgt
     5341 gcgcgtcagc aaattggcgg tgccgcggct cgacatgaag ccggcgccgc cgccgacgcc
     5401 gaggccgccg ccctcggatt tctgcagcaa caccgcgccg atcagcgtcg cgacgatcat
     5461 gaggtgaatg acgataacga cagtctgcat ctctaccttc cgtcgcaaac caaaagcgcc
     5521 gaggtccggc tgctcatttg cgcttgcggg gtttgaagtc gcgcggtgtt acacgattgg
     5581 acggggcgtt gtcacccctg ccgcgaccac cctaatataa tcacttcatc gctgtttggc
     5641 ctggttgcga tgacgaaatc cgccccaggg ccaatcacac ttattgtatt gccttctcac
     5701 caatcgtcta ctataataga cgaatgtctc tcttgctaga cgatacatcc tcccgcattg
     5761 ccgggcgttt gcgacttgaa cgcgaaaatc gcggctggtc tctggcggat ctcgccgaac
     5821 gctcgggcgt atccaaggcg accatcagca agatcgaacg caaggaggtc agcccgaccg
     5881 ccgtgatcct ggttcggttg gcgggcgcgt tcgacctgac cctggcaggc ttgctgctgc
     5941 gggcagaggg ccagggacgg ctcacccggg ccacagaaca gccggtgtgg cgcgaccccg
     6001 acaccggcta tttgcgcaag caagtgttcg gccggcccga ccatcctgtc gagatcatta
     6061 gggttgagct gccggccgga cagcgggtgg tgctcccggc ttcgtcttac gtgcatattc
     6121 gtcaggccgt ctgggtgcag gcaggccatc tcgtcatcat cgaagccggc gagcggcgcg
     6181 aactcggaac gggcgactgc cttggatttg gcccgccgtc cgaagtcacc ttcgccaacg
     6241 aaacgaccgc ctggtgcgtc tatatcgtcg tcctggctcg gagctgacgg tcatgtcgga
     6301 gatcgacatc aagccccgtg caaccgaacc aaagagtcaa gccagcggcc ttaccgccgg
     6361 ccacaatgga gggccgccgc tgcacgaagc acacaagccg gagtggggcg aagggccgat
     6421 cggaaactat ttctactgga aagctgcgca tcgcacggct tggcacagcg cgtcgcgtga
     6481 cgtgatcctg tttcgcattc aaagagccga acggctgggg ctcacatatg aagagtacac
     6541 tttggaaatt ctcgaacgag gccgccatct tcaaatcgag gatgtcgaaa gaattgccc
//
LOCUS       DASWGM010000313         3869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2249165,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000313 DASWGM010000000
VERSION     DASWGM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2249165"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VGA15_09040"
     CDS             <1..952
                     /locus_tag="VGA15_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587877.1"
                     /translation="LIMLGLHLSYPFLIASAVLLGLANSVYHPADYAILSAHMDEARM
                     GRAFSIHTFAGFLGGAVAPAIVAALVATTGGLGALIVAGAVGPLVALLLLIVGIPDAS
                     AADRHVDGAPAPQQNIITPALMVLTIFFMLLSLSSAGIGNFGVVALMSGYGASFSSAN
                     IALTAFLGFSAIGVLAGGFLADRTRRHGQVAAACFAINAAIVFIIATATLPSLPLMTA
                     MATAGFLGGVIAPSRDMLVRSAVPAGAAGRAFGIVSTGFNLGGIVSPLLFGWIMDQNM
                     PHWVFGASVIFMILTVLLALFSDRGPQQRPAGADARLMHS"
     gene            1030..2772
                     /locus_tag="VGA15_09045"
     CDS             1030..2772
                     /locus_tag="VGA15_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9587878.1"
                     /translation="MADHPDLVIRGGNIADGLGGELFEADVAIADGGIVEVGRVLSKG
                     AEEIDARGKLVAPGFVDLHTHYDGQVTWSHDITPSSQNGVTTAVMGNCGVGFAPCRPS
                     DHQRLIQLMEGVEDIPEPVLSAGLPWAWESFPDYMDWLSQRNFDMDIGAQLPHAALRV
                     YVMGERGARRDPSTPQDNGAMAALAGDAVRAGALGFSTSRTLNHRTSTGDFTPTLKAG
                     EDELTAIAGAMHKVGRSVLQFVLDQSTIDEDLPMMLRVAENTKCPISFSIVQADKAPR
                     RWRQTLDTINEASKRGLSITAQIAARPVGLLLGLELSRNPFQTHPSYKAIARLPLAER
                     VARLRQPEVRAAILSETATSTDDPLFFRPNYDKMYLLGDPPDYEQPLENALGAQARRQ
                     GRQPEELAYDAMLSDNGRGMLYVPFLNYADGNLDAVREMLRDPHSVPGLSDGGAHCGI
                     ICDASFPTYLLTHWTRDRTRGEKLSIPFVVAAQSRKTALSVGLYDRGVIAPGYKGDVN
                     VIDYDRLHLHPPKVHYDLPVGGRRLLQQVDGYDATIVSGVVTQRRGSATGARPGRLVR
                     GAQGSAPRIEAAAS"
BASE COUNT          687 a         1257 c         1205 g          720 t
ORIGIN      
        1 gctgatcatg ctcggcctgc atctgagcta tccgttcctg atcgccagcg ccgtgctgct
       61 ggggctcgcc aacagcgtct atcatccggc cgattatgcg atcctttcgg cccacatgga
      121 tgaggcgcgc atgggccgcg ccttttcgat ccacaccttt gccggtttcc tcggcggcgc
      181 ggtcgcgccc gccatcgtgg cagccctggt tgcaacgacc ggaggcctcg gcgccctgat
      241 cgtggcaggt gcggtgggtc cgctggtggc gttgctgctc ctgatcgtcg gcattcccga
      301 tgccagtgcg gccgatcgcc acgtcgatgg ggcgccggca ccgcagcaaa acatcatcac
      361 ccccgccctg atggtgctga cgatcttttt catgctgctc agtctttcca gtgccggcat
      421 cggcaatttc ggcgtggtgg cgctgatgag cggctatggc gcatcgttct cctcggccaa
      481 tatcgcgctg accgcgtttc tcggctttag cgccatcggt gtgctggcgg gcggctttct
      541 cgccgaccgc acccggcgcc acgggcaggt cgccgccgcc tgctttgcga tcaacgcggc
      601 catcgtgttc atcatcgcga cggcgacgtt gccttcactg ccgttgatga cggcgatggc
      661 aacggccgga ttcctcggcg gcgtgatcgc gccctcgcgc gatatgctgg tccgcagcgc
      721 cgtgcccgca ggcgcggccg gccgggcctt cggcatcgtc tcgaccggtt tcaacctcgg
      781 cggcattgtc agcccgctat tgtttggctg gatcatggac cagaatatgc cgcactgggt
      841 gttcggcgcg tccgtcatat tcatgattct cacggtcttg ctcgcgcttt tctccgaccg
      901 cggcccgcaa cagcgccccg caggggcaga cgcaaggctt atgcattcct gagcctcatc
      961 ccgggcggtt gacagcgacg cgtggcaacc gatgatgcgg caacaaaatt cagctcggga
     1021 agaaacacca tggccgatca tccagacctc gtgatccgcg gcggcaacat cgccgacggg
     1081 cttggcggcg aactgttcga agcggatgtc gcgatagcgg acggcggcat tgtcgaagtc
     1141 ggccgtgtac tcagcaaagg tgccgaagag atcgacgccc ggggtaaact ggtggcgccg
     1201 gggtttgtcg acctgcacac ccattatgac ggccaggtca cctggagcca cgacatcacg
     1261 ccctcgtcgc agaatggcgt caccaccgcc gtgatgggca attgcggcgt cgggttcgcg
     1321 ccctgccgtc cgtccgacca tcagcggctg attcaattga tggaaggcgt cgaggatatt
     1381 cccgagccgg tgctcagcgc cggcctgccc tgggcctggg aaagttttcc ggactacatg
     1441 gactggctgt cgcagcgcaa cttcgacatg gacatcggcg cccaattgcc gcacgccgca
     1501 ctgcgcgtct acgtcatggg cgaacgcggc gcgcgccgcg atccctcgac accgcaggac
     1561 aacggcgcga tggcggcgct cgcgggcgat gccgtgcggg ccggcgcgct gggcttctcg
     1621 acctcgcgca ccctcaacca ccgcacctcg accggcgact tcacgccgac gctgaaggcc
     1681 ggcgaggatg aactgacggc gatcgccggc gccatgcaca aggtcggccg cagcgtgctg
     1741 caattcgtgc tcgatcagag caccatcgac gaagacctgc cgatgatgct gcgggtggcg
     1801 gaaaatacca aatgtccgat ttcgttttcc attgtgcagg ccgacaaggc gccgcgacgc
     1861 tggcgccaga cgctggacac gattaatgag gcctccaagc gcggtctgtc gatcacggca
     1921 cagattgccg cgcgcccggt ggggctgttg ctgggactgg aattgtcgcg caacccgttc
     1981 cagacccatc caagctacaa ggcgatcgcc aggctgccgc tggctgaacg cgtggcgcgc
     2041 ctgcgtcagc ccgaggttcg cgcggcgatc ctgagcgaga cagcgacctc gaccgacgat
     2101 ccgctgttct tccgtccgaa ctacgacaag atgtacttgc tcggcgatcc cccggattac
     2161 gaacagccgc tggaaaatgc gctgggcgcg caggcccgcc gccagggacg gcagccggaa
     2221 gaactggcct atgacgcgat gctgtccgat aacggccgtg gcatgctgta tgtgccgttc
     2281 ctcaattacg ccgacggcaa tctcgatgcc gtgcgcgaaa tgctgcgcga tccgcattcg
     2341 gtgccgggac tgagcgatgg cggcgcccat tgcggcatta tctgcgatgc cagcttcccg
     2401 acctatctgc tgacgcactg gacccgcgac cgcacccgcg gcgaaaagct gtcgatcccc
     2461 ttcgtggtgg cggcgcagtc gcgcaagacc gcactttcag tcgggctgta cgatcgcggc
     2521 gtgatcgcgc ccggctacaa gggcgatgtc aatgtcatcg actacgacag actgcatctg
     2581 catccgccaa aagttcatta cgatcttccg gtgggcggcc gccgcctgct gcaacaggtc
     2641 gatggctatg acgccaccat cgtctccggc gtggtgacgc aacggcgtgg gtcggcgacc
     2701 ggcgcgcgtc ccggccggct ggtgcgcggc gcacagggca gcgcgccgag gattgaagcc
     2761 gcggcttcgt agcgcatggt ccagctggat tgaagctggc tcgatgtccc ggcaatgacg
     2821 gcgccctttc tctcctcttg gaccccacaa gaggagagag cgcgcgccgt tgcggcggcc
     2881 tcccatcgga gctttatccg gatggatcag cctcggtgca aattccagat tcaattttca
     2941 aacagccaag agaagaccgt cattgcgagc gcagcgaagc aatccatggc cgccgcgaaa
     3001 aagaaagatg gattgcttcg ctgcgctcgc aatgacggca aatcgcagtt ccaaatacac
     3061 gacttcgctc tcgcgacgcg aagcgcccga gtcgttgcat ttatctttcg cccccggaga
     3121 aaatagaggg cgtggggaat gccgggtgcc cgctgcaccc gcagcctcgt gcgcgctttg
     3181 tagtagtaga agcgcacgag taacgacgag tacaccggaa tcgcccggca ttcccgcacg
     3241 caatggtttt aacggcgtat gtcgcgctct ccccggtgac cgggctcttt tgccaccgtc
     3301 gtcttgcgaa tatggaatgt ccgcgcccgg tagggccgac atgcctccac aaaacttgac
     3361 accagcgtcg gggcgtcagg accacacgat ttggccgtcc gcgacaacat ctctcgtccg
     3421 cgtgcttaag gcgtcgctca cggatctcgt cgatccgccc tgcgatccct cgcccgcaag
     3481 acgctgccgc gtccaccgca tcccgcaccc aacgtccgtg acgatcgcga tacgcccctc
     3541 tctcgggccg ggatggtgag cgtgatagag atgatttggg gggaacggga agcggaatat
     3601 ttttgcggag ggggactgga caggtagaaa caggttgatc ctgttcaaga aattaggctg
     3661 cgcgcgcacg tggtgtaggc ggggcggagc gccgaaaaat ccaggatctc aacttccaaa
     3721 gccgattgaa tgcagcgtca ccgtaattcg agaccttgaa acacgtccaa ggtggcccca
     3781 tcaacccgcg aaggcgtttc tcggcagccg cgaacgctct aaactcactt ccgctctcag
     3841 tccacagcgg acatagccgg acttgttgc
//
LOCUS       DASWGM010000314         1986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2388028,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000314 DASWGM010000000
VERSION     DASWGM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388028"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="VGA15_09050"
     CDS             <1..1613
                     /locus_tag="VGA15_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587879.1"
                     /translation="AEKTNAVPKPRPITSARIAALKVVIALPSIRQAGYDCPAILSIF
                     MIRAACKTIAMTTVAMSTKLLNPSRSLVVAVGLTAYASVLARGGEVLIDPDVYMHIVV
                     GRWILDHGALPYADTFSHSMHGMPWVPHEWLAEVVFAWLYDHFGWTSQVVVTALAVGL
                     AVALLARALIYYLTPVYAVIGAMLAWGMCFPHLLARPHVFVYPLVVLWAAGLVAARQE
                     NRAPSPLLLIVLVLWANVHGSFLFGIVLAALFAAEALLEAADWPAARRVVYGWGLFGL
                     LSLVAAFATPNGLAGFLLPIKTVRMSFAMSWISEWQSPNFQQFQPLELWLMLVLVGAL
                     LLGVRLPITRIAIMLVLLHMTLQHLRHGEYLGFITPLLVAPALALQLPRSLEDLGMPA
                     LRVKLPSGATFALGALALAMVATGARMGMMRTIDRVTPSAALAAAREHQVAGPVFNDF
                     NFGGFLIFSGVPTMIDGRTDLYGDAFNRRYSRIEELPRLLGEYRIGWTLLRAGTPGAV
                     LMDYLPGWQRLYADDIAVLHARKGAADR"
BASE COUNT          305 a          617 c          632 g          432 t
ORIGIN      
        1 aggcggagaa aacgaacgcc gttcccaaac ccagaccgat taccagtgcg agaattgcgg
       61 ccctcaaagt ggtcatagct ctgccctcca tccgacaggc agggtatgat tgccctgcca
      121 tcttgtcaat attcatgatt cgggcagcat gtaaaaccat tgcaatgacg accgtcgcaa
      181 tgtctacgaa gctcctgaat ccctcgcgca gcctggttgt cgccgttggg ctcaccgcct
      241 atgcgagcgt tcttgcccgc ggcggcgagg tgctgatcga tccagacgtc tatatgcaca
      301 tcgttgtggg gcgctggata ctcgatcacg gcgccctgcc gtatgcggat acgttctccc
      361 acagcatgca cgggatgccg tgggtgccgc atgagtggct cgccgaggtc gtgttcgcgt
      421 ggctttacga tcatttcggc tggaccagcc aggtggtggt gacggcgctg gcagtcgggc
      481 ttgcagtcgc gcttctcgcc cgcgcgctca tctactacct gacgccggtt tatgcggtga
      541 tcggagcgat gctcgcctgg ggcatgtgtt tcccgcatct gctggcgcgc ccgcacgtgt
      601 ttgtctaccc gctggtggtg ctctgggcag ccgggcttgt cgccgcgcgc caggagaacc
      661 gggcaccttc gccacttctt ctgatcgtgc tggtgctatg ggcgaatgtg catggcagct
      721 tcctctttgg gatcgtgctc gccgcgctgt ttgctgccga ggcgttgctg gaagctgccg
      781 attggcccgc cgcccgccgc gtcgtgtacg gctggggcct gttcggcctg ctgtcgctcg
      841 tggccgcgtt cgccactccc aacggccttg cgggattcct tttgccgatc aagaccgtcc
      901 gcatgagctt tgcgatgtcg tggatttccg aatggcagag tcccaacttc cagcaattcc
      961 agccgctcga gctgtggctg atgctggtac tggtcggcgc gctgttgctc ggcgtgaggc
     1021 tgccgatcac gcgcattgcc atcatgttgg tgcttctcca catgacgctg cagcatctgc
     1081 gccacggcga atatctcggc ttcatcacgc cgctgctggt cgcgcccgcg cttgccctgc
     1141 agctcccacg cagcctggaa gaccttggca tgccggcgtt gcgcgtgaag ctgccgtcgg
     1201 gcgcaacctt cgctttaggc gcgctcgcgc ttgccatggt cgcgaccggc gcgcgcatgg
     1261 ggatgatgcg caccatagac cgggtcactc cgtctgcggc gcttgcggcc gcccgagagc
     1321 atcaggtggc cggtccggtg ttcaacgatt tcaatttcgg cggctttctg atcttctcgg
     1381 gggttccgac gatgatcgat gggcgcaccg atctttatgg cgatgccttc aacaggcgtt
     1441 acagccggat cgaggagctc ccgcggttgc tcggtgaata caggatcggc tggacgctgc
     1501 tgcgcgccgg caccccgggc gcggtgctca tggactatct tcccggatgg cagcggcttt
     1561 atgccgacga catcgccgtg ttgcatgccc gcaagggtgc agcggatcgc tagcgcatga
     1621 cgtactctag cggcatgcgg ccgcggggat ttcccgccgc gactttctca aggtgtggcg
     1681 ctgacgaacg cgtcctagct ggcgcgtttg aaccaaggta agaaacgtcg ttcccgtgaa
     1741 agcgaggacc tatatccgca ggcggcggtg ttatgcgtcc cgacgcgcgc ggggacgacg
     1801 gctgcggagc gatcgtggta gatttaaagt tccttcgcgc ctgacccgat cctgaatccg
     1861 aagtgcgttt cgataggcgg cgatcagtga tcacctcgga cccgtctacg tcggggtgag
     1921 aaccgccgaa cgatgctcgc acagcaacgc ccgcggcgct caccaggacc tgagaacgtt
     1981 ttgcgt
//
LOCUS       DASWGM010000315         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_575321,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000315 DASWGM010000000
VERSION     DASWGM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575321"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VGA15_09055"
     CDS             complement(<1..1244)
                     /locus_tag="VGA15_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9587880.1"
                     /translation="MPTECIPDLFGFEPVEGRDVVAAFDAGMVTSDAGALLLGATDRA
                     IKMMERFASCFHDVRRAELIEHEVATLVGQRVFGIALGYEDLNDHDELRHDPMMAVLA
                     GKLEARRADCAPVAGKSTLNRLELSRLEPTRYHKISHNPMAIKRLFVDLFLEAHGRPA
                     KQIILDLDATDDPVHGNQEGRFFHGYYDCYCYLPLYVFCGRDLLAAKLRRASVDAAAG
                     AVEEVARIVAHIRRQWQRVRILVRADSGFAREELMAWCEANGVDFLFGLAKNDRLNAM
                     IKNELEQAAAISQRTGKPARRFKQFMWTTRNSWSHQRRVVAKAEWTQGEANPRFVVTS
                     LTQAECKARYLYEKIYCARGDMENRIKECQLDLYADRTSTATMRANQMRLWFASMAYV
                     LLCALRRIGLHDTEYAKATCGT"
     gene            complement(1371..>1681)
                     /locus_tag="VGA15_09060"
     CDS             complement(1371..>1681)
                     /locus_tag="VGA15_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587881.1"
                     /translation="RHGAHVCVSLFPLFWRPRRRRFDALGSDSQHFPSLAKPVRREMT
                     FSPARARSSVLIDMPLAFAFSSRSVAATSPSASSRRRKAMAWSIRFGSFRFWPIDFTL
                     "
BASE COUNT          340 a          546 c          496 g          299 t
ORIGIN      
        1 atggtgccgc aggtcgcctt ggcgtattcg gtgtcgtgga gtccgatgcg gcgcaaggca
       61 cagagcagca cgtaggccat cgaggcaaac cacaggcgca tctggttggc tcgcatcgtg
      121 gcggtcgagg tacgatcggc atagagatcg agttggcatt ccttgatgcg attttccatg
      181 tcgccgcggg cgcaatagat tttctcgtaa agatatcgag ccttgcactc cgcctgcgtc
      241 agcgaggtga ccacgaagcg ggggttggct tctccttgcg tccactcggc cttggccacg
      301 acgcggcgct ggtggctcca actgttgcgc gtcgtccaca tgaactgctt gaagcgccgc
      361 gccggcttgc cagtccgctg gctgatcgcc gccgcctgtt caagttcgtt cttgatcatg
      421 gcgttgagcc ggtcgttctt ggccaggccg aacagaaagt ccacaccgtt ggcctcgcac
      481 caggccatga gttcctcccg cgcgaacccg ctgtcggcgc gcaccagaat gcgcacccgc
      541 tgccattggc ggcggatgtg agcaacgatg cgcgccacct cctccaccgc acccgccgcg
      601 gcatctacgc tcgcgcgccg cagcttggcg gccaacagat cgcgcccgca gaacacgtaa
      661 agcggcaggt agcagtagca gtcgtaatat ccgtggaaga accgaccctc ctggttcccg
      721 tgcacaggat catcggtcgc atcaagatca aggatgatct gctttgcagg tcgcccgtgc
      781 gcctccagga acaggtccac gaaaagccgc ttgatggcca tcgggttgtg actgatcttg
      841 tgatagcgcg tcggctccaa tcggctcagc tccagccggt tcagcgtcga cttgccggcc
      901 accggcgcac aatccgcgcg ccgcgcttcg agcttgcccg ccagcaccgc catcatcgga
      961 tcatggcgca gttcgtcatg atcgttcaga tcctcgtatc caagcgcgat cccgaacacc
     1021 cgctgcccca ccagggtcgc gacctcgtgc tcgatcaatt ccgcacgccg cacatcgtga
     1081 aaacacgacg caaaccgctc catcatcttg atggcgcgat ccgtcgcgcc gagcagcagc
     1141 gctccggcat ccgacgtgac catgccggca tcaaacgccg ccaccacatc gcgtccttca
     1201 accggttcaa atccaaacaa atccggaata cactctgtcg gcatcggggc gctccgcgat
     1261 tcggcgagaa atagttgtag cacaacactt ttcgctgatt cgccgccccg atgcactcaa
     1321 ttcggtgaga aatccgcgct aggtcgcggc acagccgatc gagcggcgcc tcacagcgtg
     1381 aagtcaattg gccagaagcg aaaggagcca aagcggatcg accaagccat agccttcctg
     1441 cgtcgcgagc ttgctgacgg tgaagtcgcc gcgaccgacc tggaggaaaa ggcaaaggcc
     1501 agcggcatat caatgaggac gctcgatcgc gcacgtgccg ggctgaaggt catctcgcgg
     1561 cgcacgggct tcgccaaaga ggggaaatgc tggctgtcgc tgcctagggc gtcaaaacgg
     1621 cggcgcctag gacgccaaaa aaggggaaag agagagacac acacatgtgc accgtgtcgg
     1681 c
//
LOCUS       DASWGM010000316         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_32285,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000316 DASWGM010000000
VERSION     DASWGM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32285"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VGA15_09065"
     CDS             <1..863
                     /locus_tag="VGA15_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX9587882.1"
                     /translation="RHALGRLLTALSTVGICSRVGADGYSLTEVGAALDSSAEKSFKA
                     WAIFEGDMLFKSWHGMLETVMTGKTAAQLLGAGNSFELMARTPANVGIFNAAMADLTR
                     LVTPDVLGAYDFSRIDCLMDVGGGSGELIGAVMKEYPGIRGIVFDLPRCTEAANAHLE
                     RVGVSDRASFVAGDFFDSIPKLADAIILKSVIHDWNDERSAVILRNCRRALPENATLL
                     LVERLMPALPGTGDEDRAHAMNDLNMLRGPGGLERTEQEYRRLLDDCGFHPIAVYPAG
                     RFSVIEARVR"
     gene            complement(885..>1821)
                     /locus_tag="VGA15_09070"
     CDS             complement(885..>1821)
                     /locus_tag="VGA15_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587883.1"
                     /translation="CLLLLTSTRACVGDAEAEFSQRLAAPIVKAGLVTDDSSPAPVDP
                     AVEEQKACAGVAFSRSLKYGESELNVLDVATGDTKEPARRPVLLFVAGESFVGESSTP
                     DVAGSMQDEAMCFAARHGMVGAKMAYRLAPAHPWPAGARDVAAAISWVHQNIDLFGGN
                     PDEIVAVGYSAGAFHVASFLGHPEFQERDSNVAGAVLVSGLYRLSADAGAAEKSYFGA
                     DPSKYAERSAFPGILDTETPLLVAWSATDPPHLVAQGEKLRELLCKAPAHCPHTTVLS
                     ARDSLASVFGLDASSSSLAEPTLELVREIEARGLP"
BASE COUNT          339 a          549 c          585 g          348 t
ORIGIN      
        1 atcgccacgc actggggcgt ctgctcaccg cgctttcgac cgtcggcata tgttcacggg
       61 tcggcgcaga tgggtattcg ttgacggaag tgggcgcagc actggacagc tccgcggaga
      121 agtccttcaa ggcctgggcg atcttcgaag gcgacatgct tttcaagtcc tggcatggaa
      181 tgctggagac cgtcatgacc ggcaagaccg cggcgcagct cctcggcgcc ggcaacagtt
      241 tcgaactgat ggcgcgcacg ccagcgaacg tcggcatatt caatgcggcg atggccgacc
      301 tgacgcgtct cgtgacgccg gacgtgcttg gggcctatga tttcagccgc atcgattgcc
      361 tgatggatgt aggcggcggc tccggcgaac tcatcggtgc cgtgatgaag gaatatcctg
      421 gtatccgcgg gattgttttc gatcttccgc gctgcactga ggccgcgaac gctcatttgg
      481 aacgggtggg cgtcagtgac cgcgcgagtt tcgtcgccgg cgatttcttc gattccattc
      541 caaaactcgc cgatgccatc atcctcaaaa gcgtgattca cgattggaac gatgaacgta
      601 gtgcggtgat cctccggaat tgccgtcggg ccctgccgga aaatgcaacg ttgctgctgg
      661 ttgaaagact gatgccggca ttgcctggca ccggcgacga ggacagggca catgccatga
      721 acgatctgaa tatgctgcgc gggcccggcg gccttgagcg caccgaacag gagtaccgcc
      781 gactactcga tgattgcggc tttcatccaa tcgcggttta tccggctggc cgcttcagcg
      841 tgatcgaagc gcgcgtgcgt tgatacctca gggaaggcgc cttttcaggg aaggcctcgg
      901 gcttcgatct cccggaccag ttccagcgtc ggctcggcta ggctgctgct cgaggcatcg
      961 agcccaaaca cggaagccag gctgtcgcgg gcgctcagca ccgtcgtgtg cgggcaatgg
     1021 gcgggtgcct tgcacaaaag ctccctgagt ttttcgcctt gcgcaacgag gtgtggcggg
     1081 tcggtcgcgg accaggcgac cagcagcggc gtttcggtgt cgaggatgcc gggaaatgcc
     1141 gatcgttccg cgtatttgct ggggtcggcg ccaaaatagg atttttcggc ggcaccggcg
     1201 tctgcgctca aacgataaag gcctgatacc agtaccgcgc cggcgacatt ggaatcgcgc
     1261 tcctgaaatt ccgggtggcc aaggaaactc gccacgtgga acgcgccggc ggaataaccg
     1321 accgccacga tctcgtcggg attgcccccg aacaggtcga tgttctggtg gacccacgag
     1381 atcgccgccg cgacatcccg ggccccggcg ggccacgggt gtgccggagc gagccgatag
     1441 gccattttcg cgccaaccat tccgtggcgc gcggcaaagc acatggcttc gtcctgcatt
     1501 gagcccgcga catcgggagt gctgctttcg ccgacaaaac tttcgcccgc cacgaacaac
     1561 agcacggggc gccgggctgg ttccttggta tctccagtcg cgacgtcaag cacgttgagc
     1621 tcgctttcgc cgtacttcag gctgcgggaa aacgccacac cggcgcaggc cttctgttct
     1681 tccacggcgg ggtcaaccgg cgcggggctg gaatcgtcgg tgacgagtcc agccttcacg
     1741 attggagccg caagccgttg cgaaaactcg gcttcggcgt cgccaacgca agcacgcgtc
     1801 gacgtcagga gtaaaagaca a
//
LOCUS       DASWGM010000317         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2388379,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000317 DASWGM010000000
VERSION     DASWGM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388379"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VGA15_09075"
     CDS             complement(<1..516)
                     /locus_tag="VGA15_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9587884.1"
                     /translation="MKNKKRSLLASHLIAVAAATALSVLATQGALAQKKYDTGATDTE
                     IKIGNVEAYSGPASAYGIIGKTEDAYFRMINDQGGINGRKINFISYDDGYSPPKTVEQ
                     VRKLIESDEVFLVFNALGTPTQNAVQKYHNAKKVPQLFLATGASKWNDPKEYPWTMGF
                     QPSYRVEARIFA"
     gene            complement(576..2129)
                     /locus_tag="VGA15_09080"
     CDS             complement(576..2129)
                     /locus_tag="VGA15_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9587885.1"
                     /translation="MPVRHYDWIAHFARRTPDKLAVVDLASERRLDYAQFDARISHLA
                     THLRDGVKIARGDRVAVLTHNTTDTLEVQFACGRIGAVFLPLNTRLTVPELQYIVGDA
                     APKLMIHDAALADLALAVAKRCQVPSALLLGAGGSYEAAIAAARPLDHAEIVTLDDMS
                     TIMYTSGTTGQPKGAIITHGMTFWNCVNLGGPAYISPSSVLLTVLPLFHTGGLNCYTN
                     PVLHAGGTVLIMRAFDPGLALQLMSDPSHRINTFFGVPSIYQFMAQHPAFAGSDFSRL
                     VICGVGGAPMPVPLLKVWEERGVALQQGYGMTETSPAVLTLDREDAARKAGSSGKPVL
                     HTEVRIVRPDGSDADIGELGELWVKGPNITPGYWNRPDANQSSFTDGWLHTGDAARID
                     QEGFYYIVDRWKDMYISGGENVYPAEVENVLHQLAAIAEAAVIGIADAQWGEVGMAIV
                     AVKPGHTLTEAEIQAHCEANLARFKRPSVIRFVDALPRNATGKIHKPTLRKNFGLPTA
                     TDSANAGVP"
BASE COUNT          435 a          782 c          823 g          502 t
ORIGIN      
        1 cgcgaaaatc cgggcctcga cgcggtagct cggctgaaag cccatggtcc agggatactc
       61 tttcgggtcg ttccacttgc tggcgccggt ggcgaggaag agttgcggga ccttcttggc
      121 gttgtgatat ttctgaaccg cattctgggt cggcgtgccc agcgcgttga agaccaaaaa
      181 tacttcgtcg ctctcgatca gcttgcggac ctgctccacc gtctttggcg ggctgtagcc
      241 gtcgtcgtaa gagataaagt tgatcttgcg gccgttgatg ccgccctggt cgttgatcat
      301 cctgaaatag gcgtcctcgg tcttgccgat gatgccgtag gccgaagccg gcccgctata
      361 ggcctcgaca ttgccgatct tgatttcggt atcggtggcg ccggtgtcgt atttcttctg
      421 cgccagcgcg ccttgcgtgg ccagcaccga aagcgccgtg gcggccgcga cagcgatcag
      481 gtgcgaggcg agcagggacc gtttcttgtt cttcatggcg agtacctttt cttgattgga
      541 gattggcgag ggacgggctg ttgggaagcg agcggtcacg gcacgccggc gttcgcgcta
      601 tcggtagctg tgggcaaacc aaagttcttg cgcagcgtcg gcttgtggat cttgccggtg
      661 gcgttgcgcg gcaaggcgtc gacgaaccgg atcacgctag gccgcttgaa gcgcgccaga
      721 ttggcctcgc aatgcgcctg aatctccgcc tcggtcaggg tgtgccccgg tttgacggcg
      781 acgatcgcca tgccgacctc gccccactgc gcatcggcga tgccgatgac cgcggcttcc
      841 gcgatcgcgg cgagctggtg cagcacgttc tcgacctcgg ccggatagac attttcgccg
      901 ccggaaatgt acatgtcctt ccagcggtcg acgatatagt aaaaaccttc ctggtcgatg
      961 cgcgccgcgt cgccggtgtg cagccagcca tcggtgaagg aagattgatt ggcgtccggc
     1021 ctgttccagt agccgggcgt gatgttcggt cccttgaccc ataattcgcc gagctcgccg
     1081 atgtcggcgt ccgacccgtc gggccggacg atccgcactt cggtgtgcag caccggcttg
     1141 ccggaggagc ccgccttgcg cgcggcgtcc tcgcgatcga gcgtcaatac cgccgggctg
     1201 gtctcggtca tgccgtaacc ctgttgcaag gcgacgccgc gctcttccca cactttcagg
     1261 agcggcaccg gcatcggcgc gccgccgacg ccgcagatga cgaggcggct gaagtcggac
     1321 ccggcgaacg ccggatgctg cgccatgaac tgatagatcg agggcacgcc gaaaaacgtg
     1381 ttgatgcggt gagatgggtc actcatcaat tgcagcgcca ggccgggatc gaacgcgcgc
     1441 atgatcagaa cagtgccgcc ggcatgcagc accgggttgg tgtagcaatt cagcccgccg
     1501 gtgtggaaca gcggcagcac ggtgagcagc accgacgacg gcgagatata ggccgggccg
     1561 ccgagattga cgcaattcca gaaggtcatg ccgtgggtga tgatcgcgcc cttcggctgg
     1621 cccgtggtgc ccgaggtgta catgatcgtt gacatgtcat cgagggtgac gatttccgcg
     1681 tggtcgagcg gtcttgccgc cgcgatcgcg gcttcatagg agccacctgc accaaggaga
     1741 agcgccgagg ggacctggca ccgttttgca acggcaagcg cgagatccgc caacgcggcg
     1801 tcatggatca tcagcttcgg cgcggcatcg ccgacaatgt attgcagctc gggcacggtg
     1861 aggcgggtgt tcaacggcag gaacaccgcg ccgatccgcc cgcaggcgaa ctgcacctcc
     1921 agcgtatcgg tggtgttgtg cgtcagcacc gcgacccggt cgccgcgcgc gattttcacc
     1981 ccgtcgcgca gatgggtggc cagatgcgaa atccgcgcgt cgaactgcgc gtagtcaagc
     2041 cggcgctcgc tggcaagatc gaccaccgcc agcttgtccg gcgtccggcg ggcaaaatgc
     2101 gcgatccagt catagtggcg aaccggcaaa atctcctccg aaggtccggc ggtctggagc
     2161 cgcccgatcc tgtttgatcc tatgccatat cgcgccagtt gggacgctgt actgggatgc
     2221 tgtcttgcgt taagtggctg cgtcacttgc agacacagct tcgtcacttg cagacacagc
     2281 ttcgcgatct cgcggcgcat ttcgcccgag gtttgccagt aatttcctta ccctccgatc
     2341 agagggcgca gggaatgccg ggcgcccgat gcgcccgcag ccgcgcgtgt agtgtagtaa
     2401 gcacgcgcgt tagtcaccac ggtcacaccg gaaaacgccc ggcattcccc gcgcaatggt
     2461 tttaacggtt tgcttcgtgc tctccccggt gaccgggctt gtttgccacc gtcatcgacg
     2521 gattgctccg ccaacttgac ac
//
LOCUS       DASWGM010000318         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_159236,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000318 DASWGM010000000
VERSION     DASWGM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159236"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..611)
                     /locus_tag="VGA15_09085"
     CDS             complement(165..611)
                     /locus_tag="VGA15_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587886.1"
                     /translation="MRFPAPLHRKKELKDRIMKTGKRIVISAFGGALCLAASTGLSAA
                     ESWSPTTMKPLMAKSLDVGSKHVVSYFLNADGRCKLTLMIADAARDENSERPAQILRL
                     RLMVEPGRAALVDTTDGKLLQFACEPDALAMHATLLDQIAAGRDIK"
     gene            complement(888..1394)
                     /locus_tag="VGA15_09090"
     CDS             complement(888..1394)
                     /locus_tag="VGA15_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587887.1"
                     /translation="MRAKLPSKSGFVDRDGVKIYYEIYGDGPETMVFLPPWPIVHSRI
                     YKAQLPYFSERFRCITFDARGNGKSDRPADAAAYSLEQYVADALAVMDATEVGNAILV
                     GLSSGGMHASVLAAHHPERVKAAILVGTVASIGPANFPCLATKHFEAKREQFDGWDKF
                     NREYWMAN"
     gene            complement(1399..2037)
                     /locus_tag="VGA15_09095"
     CDS             complement(1399..2037)
                     /locus_tag="VGA15_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9587888.1"
                     /translation="MARESAKKIENKVEKKGETSTTLLEAAKKVLRQNGYAGLSTRDV
                     AAAAGVPLSQIHYHFGSKQGMVLALFEYLNAQLLDRQNAMFGDASLKLSEQWDRACDY
                     LDDDIASGYVRVLQELITASWADAAVAQVVRTALMAWVDLIVGAARKAERELGGLGPL
                     TPEEVGAFAANAFIGAESLYLLGFEKKGSPIRQALRRIGDLVRSAEASSSKR"
     gene            2244..2588
                     /locus_tag="VGA15_09100"
     CDS             2244..2588
                     /locus_tag="VGA15_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEX9587889.1"
                     /translation="MRLKSSIWVAAYLRRCQTQGIFGAVRRRGAEEAGAVFVKVVLLD
                     GNAMLYVPAPQTVYDDSRPIERIFTPTSREPVPEPSVEERLVKEIRFDPDAWIVETED
                     KAGRHFLDLAKA"
     gene            complement(2717..3673)
                     /locus_tag="VGA15_09105"
     CDS             complement(2717..3673)
                     /locus_tag="VGA15_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX9587890.1"
                     /translation="MFPGFDGLMTLSRREGVDIRPTLLRVLTDLYVQASVHSDDEERQ
                     FVELTSRLIDEVDDATRAVVRARLAIYPGTPVEVLRRLGLQRSSTNEIVPPAPAIAAP
                     PSAAPPVKAPTEAQSRMSSNLTMQPKDAAEITDLFFGASSSERALILQNLLETPLKAS
                     ARIPTARAARAIETLEMAAFAADVENFTLELGETLILPWRLAAQVVNDAGGEPLACAA
                     RALDMPSPVFQRVLLFLNPEIGSSVNHVYRLSRLYDGLHERSALIMLAAWRGATMAVA
                     RAKYRPGLYDDERNRVRSAPAQTRPATQWPGTGGVVRTRSQG"
BASE COUNT          694 a         1109 c         1126 g          747 t
ORIGIN      
        1 gcatcgtcgg cgacttcata gcccaggtcg atgccggccg ctggtccacg cgtgacccgc
       61 gcgccatgtc ggactcgata accggcattc gctgatgatg cggacaaggg ctcaacgtgc
      121 acccgcgcgg cgcggcgccc attctggatg ccgcgaatga aggattattt gatgtcgcga
      181 ccagcggcga tctgatcaag cagagttgcg tgcattgcca gcgcatcggg ctcgcaggcg
      241 aactgaagca atttgccatc cgtggtgtcg actagcgccg cccttccggg ctcgaccatc
      301 aatcgaaggc gcaagatttg cgccggacgt tcgctgttct cgtcacgagc ggcgtccgcg
      361 atcatcaacg tcaatttgca tcgaccgtca gcattgagga aatagctcac gacatgcttc
      421 gatcccacat cgagactttt tgccatcagc ggcttcattg tcgtagggga ccaggactcg
      481 gcagcggata ggcccgtgct tgctgcaaga cacagcgctc ccccgaacgc cgagataacg
      541 atacgctttc ctgttttcat tattctatcc ttcaattcct tttttcgatg aagcggggcg
      601 gggaaacgca ttcatgactt ctatccccac gaatccgcgt catcgccatt ccatcctagg
      661 cggctgccat cattatgctg cagtgcatca tatcttaaat tgcattgcaa catattaatg
      721 ttgcggcgcg aaaaatttcg gcgtccctag ggtttcgggt tacagaggtc tgtttgaaaa
      781 ttacgttcac gaaacaacgc tcgccatggc ttatattagc cacgcgtgtt gaagacgatt
      841 gtgaggaaaa gggagatcga atccctagct gcaattccgg ctgaaggcta gtttgccatc
      901 cagtattcgc ggttgaattt gtcccaaccg tcgaactgct ctcgcttggc ctcgaaatgc
      961 tttgtcgcca gacaggggaa attggcgggg ccgatgctcg ctacggtgcc gaccaggatc
     1021 gcggccttca cacgttccgg atgatgcgcg gccagcacgc tggcgtgcat gccgcccgag
     1081 gacaggccca cgaggattgc gtttcccact tcggtggcat ccatgacagc gagcgcgtcc
     1141 gccacgtatt gctccagcga ataggccgcc gcatccgccg gccggtcgga cttgccgttg
     1201 ccgcgggcat cgaaggtgat gcagcggaag cgctcgctga aatacggtaa ttgcgccttg
     1261 tagatccgcg agtggacaat cggccagggc ggcaggaaca ccatggtttc cgggccgtcg
     1321 ccgtagatct cgtagtagat cttcacgccg tcccgatcga caaaaccgct tttgctgggc
     1381 aattttgcgc gcatcacatc accttttcga tgagctggct tcggcactac gaacgagatc
     1441 gccgatccgc cgcaaagcct gtctgatggg cgagcctttc ttctcaaaac cgagcagata
     1501 gaggctttcc gcgccgatga acgcgttggc ggcgaaggct ccgacctcct cgggcgtaag
     1561 cggaccgagg ccgccgagct cacgttccgc cttgcgcgcg gcgccgacga tgagatcgac
     1621 ccatgccatg agcgcggtgc gaacgacctg ggcgaccgcg gcgtcggccc agcttgcggt
     1681 gatcagttcc tgcaggacgc gcacgtagcc cgaggcgata tcgtcgtcga gatagtcgca
     1741 ggcccgatcc cattgctccg acagttttag actggcgtcg ccgaacatgg cgttctggcg
     1801 gtcgagcagc tgcgcgttga ggtattcgaa cagcgccagc accatccctt gcttggatcc
     1861 gaagtgatag tggatctggc tcaaggggac gccggcggcc gccgccacgt cgcgagtcga
     1921 caaaccagca tagccgttct ggcgcagcac cttcttggca gcttcgagca gcgtcgtgct
     1981 ggtctcgcct ttcttctcaa ccttgttctc gatcttcttg gccgactcgc gcgccatcgt
     2041 ggaatctcct gagcctgcga ggatcgtcgc aggcctccga tctcataatt cggtcgaccg
     2101 accgagtcga tgttttcgtc gcatggtcac ggttttgtgc accgaggacg tccgcagcag
     2161 gttggatgct ctcggccggc cacaattgct tgcggtggca agccgctctg gcttcgactt
     2221 tggcgctggc ctatcgtcac cttatgcgat tgaaatccag catttgggtg gcggcttatt
     2281 tgcgccgctg ccagacccag ggaatattcg gcgcggtgcg ccggcgcggc gcggaagagg
     2341 ccggcgcggt gttcgtcaag gtggtgctgc tcgacggcaa tgccatgctt tacgtgccgg
     2401 caccgcagac ggtctatgac gacagccggc cgatcgagcg tatttttacc ccgacatcgc
     2461 gagagcccgt gccggaaccg tccgtggaag agcgtctggt caaggaaatc cggttcgacc
     2521 cggacgcctg gatcgtcgag accgaggaca aggcaggacg gcattttctc gatctggcga
     2581 aggcttagct tactttgata atttgcgcgt attctaccgc agataagtct acgcaatctg
     2641 cgtaaacttg attgcgaagc aaaaccggta tccacccccg gatcaagtcc gagggcatgc
     2701 ttttgccgaa tacgcgctaa ccctgcgaac gcgtgcgcac aacgccgcca gtaccaggcc
     2761 attgcgtcgc cgggcgggtc tgggcgggcg ccgagcggac gcggttgcgt tcgtcgtcgt
     2821 acagcccggg acgatatttg gcgcgggcga ccgccatggt ggcgccgcgc cacgcggcca
     2881 gcatgatgag ggccgatcgc tcgtgcaagc cgtcgtacag gcgcgacagc cggtagacat
     2941 ggttcaccga ggatccgatt tcgggattga ggaacagcag cacccgctga aaaaccgggc
     3001 ttggcatgtc cagcgccctg gccgcgcaag ccagcggctc gccgccggca tcgttgacca
     3061 cctgggcggc aagccgccac ggcaatatca gggtttcgcc gagttccagc gtgaagtttt
     3121 cgacatcggc ggcgaacgcg gccatttcca gcgtctcgat ggcacgtgcc gcgcgtgctg
     3181 tgggaattcg cgcggaagcc ttcagcggtg tctccaggag attttgcaga atcaacgcgc
     3241 gctcgctgga ggaggcgccg aaaaacaggt cggtgatctc ggccgcgtcc ttcggctgca
     3301 tcgtcaaatt cgatgacatc cgtgattgcg cttcggtcgg tgccttgacc ggcggtgccg
     3361 cggatggcgg cgcggcaatc gcgggcgccg gcggaacgat ctcgttggta ctggaacgtt
     3421 gcagcccgag ccttctcagg acttcgaccg gtgtgccggg ataaatcgcc agtcgcgccc
     3481 gaacgaccgc gcgcgtggcg tcatcgacct catcgatcag gcgggaggtg agttcgacaa
     3541 actgccgctc ctcgtcgtcg ctgtgcacgc tggcctgaac gtaaagatcg gtcaggaccc
     3601 gcagcaaagt gggtctgata tcgacaccct cgcgacggga gagcgtcatc aagccgtcga
     3661 agcccggaaa caggag
//
LOCUS       DASWGM010000319         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1830374,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000319 DASWGM010000000
VERSION     DASWGM010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830374"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VGA15_09110"
     CDS             <1..363
                     /locus_tag="VGA15_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9587891.1"
                     /translation="AKFPDLDMVLIESGGDNLAATFSPELADLTIYVIDVAAGDKIPS
                     KGGPGITRSDLLVINKIDLAPHVGASLEKMDTDARRMRGERPFVMTNLRNSEGLDRII
                     GFIEAKGGLRPPAQAKVG"
     gene            669..>2221
                     /locus_tag="VGA15_09115"
     CDS             669..>2221
                     /locus_tag="VGA15_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX9587892.1"
                     /translation="MVRLGFIIGFIALIGVLLSGLAAYRVHDQELTVDGIALARAIDV
                     HASLVQDRLTERELLARVASGLFRAPSVIKANMLQPLRSSIYAFKTDFIVASWIARLK
                     PNELDAARKELATAGFSNPVIRGFDDQPLNTRSLDKPIDVLMDVEPRDAETMRFPGRS
                     YDRDPVLGPTLAQAMADGKPVASDPAPLLRPDGQIGLVLATPVLQETDASPAGFVTFS
                     YELAPLMLANDEMSLFSVVLKDPRHADGELVSDDRGIVTSRTVAPEAPTPSSIRTVTF
                     GGRDWGLAYYAKTNAVKRAEQTAAIVAAIGLALTGIVCGLFGYVAYNNLRLSREIQVR
                     IGFERRLTAVIDELNHRVKNILAVIQSIVTRTLRHGSDIDVARDLLIGRIHAMSNVVS
                     LLSESQWQGVKLKGLFEARAIPHAERIAVSGPDVAVSARAAQSLSLLFFELASHSDEG
                     LSLVGKHPHIVAHWEVTGEEPDTTFHFRWEEFNTSAATRREDSDFGVILLDRVAPEAL
                     GGVSKRYFT"
BASE COUNT          402 a          697 c          670 g          452 t
ORIGIN      
        1 gcgaaatttc ccgatctcga catggtgctg atcgaatccg gcggcgacaa tctcgccgcc
       61 acgttctcgc ccgagctcgc cgatctcacg atttacgtca tcgacgtcgc ggcgggcgac
      121 aagatccctt ccaagggcgg ccccgggatc acgaggtcgg atctcctcgt catcaacaag
      181 atcgacctcg cgccgcatgt cggcgcgtcg ctggaaaaga tggataccga tgcgagacgg
      241 atgcgcggcg aacggccgtt tgtcatgacc aatctcagaa acagcgaagg cctcgaccgc
      301 attatcggct ttatcgaggc taaaggcggt ctccgcccgc cggcgcaagc caaagtcggc
      361 tgacgcaatt cgttaacgct tgtggccacg agtcccggat gcaagcaagg ttaaaaactt
      421 ccgcgcttcg tcggaaccaa atcggctctt tgtgattaac atccttcggg cttcgccacc
      481 acgacgcgcg cggcgaccat attgatcttc cgaggcgtca tattaatcat cgccgccaac
      541 cttagttgct tagagatgat cgctgcttca ttatccccga cggttggctt tcatcctgtt
      601 tcggtttgtt gccgccccgg cggatggcag atatttcttc gccagctcct cattttctga
      661 gtaagcgagt ggttcggctg ggtttcatca tcgggtttat cgcgctgatc ggagtgctgc
      721 tctccggtct cgcggcttat cgcgtccacg atcaggaact gacggtcgac ggcatcgcgc
      781 tggcgcgcgc catcgacgtt cacgccagcc tggttcagga ccggctcacc gagcgtgaac
      841 tgctcgcccg tgtcgcatcg ggactgttcc gtgcgccttc ggtgatcaag gccaatatgc
      901 tgcagccgct gcggtcctcg atctacgcgt tcaagaccga tttcattgtc gcaagctgga
      961 tcgcgcggct gaagccgaac gaactggatg cggcgcgcaa ggaactggcg accgctggat
     1021 tttcgaatcc cgtgatccgc ggcttcgatg atcagccttt gaatacgcgg tcgctcgata
     1081 agccaatcga cgtcctgatg gacgtcgagc cacgcgatgc cgagacaatg cgtttccccg
     1141 gccgttccta tgatcgggac ccggtcctgg gaccgacgct ggcgcaggcg atggccgacg
     1201 gaaagccggt agcgtccgat cccgcaccgc tgttgcgccc ggatgggcag atcggcctcg
     1261 ttcttgccac gccggttctg caggagaccg atgcttcgcc agcaggcttc gtgacctttt
     1321 cctatgaact ggcgccgctg atgctggcga atgacgagat gtcgctgttc tcggtggtgc
     1381 tgaaagaccc gcgccacgcc gacggcgaac tggtttcaga tgatcggggc atcgtgacgt
     1441 cgcggacggt agcgccggag gcgcccacgc cgtcgagtat cagaacggtg acgttcggag
     1501 gtcgcgactg ggggctcgcc tattacgcca agaccaacgc ggtgaagcgc gccgagcaga
     1561 ccgccgccat cgtggccgcc atcggcctgg cgctgaccgg tatcgtctgc gggcttttcg
     1621 gctatgtcgc ctacaacaat ttgcggctca gccgcgaaat ccaggtgcga attggcttcg
     1681 agcgccgctt gaccgcggtc atcgacgaac tcaatcaccg ggtcaagaac atcctcgcgg
     1741 tgatccagtc gatcgtgacg cgcacgctgc gccatggttc cgacatcgat gtcgcccgtg
     1801 acctcttgat cggccggatc catgcgatgt cgaacgtggt ctcgctgctc agcgaaagcc
     1861 agtggcaggg tgtgaagctg aaagggctgt tcgaggcgcg cgccattccc cacgccgagc
     1921 gcatcgcggt cagcggtccc gatgtcgccg tgagcgcgcg cgccgcccaa agcctttcgc
     1981 tgctgttctt cgagctggca tcgcactccg acgaggggct ctcgctggtc ggcaagcacc
     2041 cccacatcgt cgcgcattgg gaagtgaccg gagaggagcc cgacaccaca ttccattttc
     2101 gctgggaaga attcaacacc agtgctgcga cccgccgtga agacagcgat ttcggcgtca
     2161 tcctgctcga tcgcgtcgca ccggaggcgc tcggcggcgt atcgaagcga tattttaccg
     2221 a
//
LOCUS       DASWGM010000320         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1695661,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000320 DASWGM010000000
VERSION     DASWGM010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695661"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VGA15_09120"
     CDS             <1..841
                     /locus_tag="VGA15_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX9587893.1"
                     /translation="DPAYHVNAGDTITIDVPEAVAAEPSGEDIALDIVYEDDDIIVID
                     KPKGLVVHPAAGHETGTLVNALIAHCGTSLSGIGGVKRPGIVHRLDKDTTGLMVAAKN
                     DRAHKSLTEQFADHGRTGAMRRGYMAFVWDLPNRQRGTVDAPIDRHPYAREKMAVRES
                     GREAITHWEIQESFNGRDAKPVAALLACQLETGRTHQIRVHLAHIGHPLLGDGVYGPH
                     FKTKASHLGPESQKALAALGRQALHAYLLALEHPRNGAVLEWISDLPEDLACLRDSLR
                     ATQ"
     gene            1060..1959
                     /gene="rpoH"
                     /locus_tag="VGA15_09125"
     CDS             1060..1959
                     /gene="rpoH"
                     /locus_tag="VGA15_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX9587894.1"
                     /translation="MARTATLPILNGESGLSRYLSEIRKFPMLEPQQEYMFAKRWREH
                     DDRDAAHHLVTSHLRLVAKIAMGYRGYGLPISEVVSEGNVGLMQAVKRFEPEKGFRLA
                     TYAMWWIKASIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDLHPDQVK
                     LIAKRLGVTDQDVVDMNRRLGGDASLNAPIRDDGEAGEWQDWLVDTSPNQEAIMAEHE
                     EFDHRREALNGAIGVLNPRERRIFEARRLAEDPMTLEDLAAEFGVSRERVRQIEVRAF
                     EKVQSAVKGTIARQEEATLHAAH"
BASE COUNT          451 a          717 c          702 g          377 t
ORIGIN      
        1 cgaccccgct tatcatgtca atgccggcga tacgatcaca atcgacgtgc cggaggcggt
       61 cgccgccgag ccatccggcg aagatatcgc gctggatatt gtctatgagg acgacgacat
      121 catcgtcatc gacaagccga agggcctcgt ggtgcatccc gccgccgggc atgagaccgg
      181 cacgctggtc aacgcgctga tcgcccattg cgggaccagc ctttccggga tcggcggcgt
      241 caagcgcccg ggcatcgtgc accggctcga caaggatacc accgggctga tggtggcggc
      301 gaagaacgac cgggcgcaca aatcgctgac cgagcaattc gccgaccacg gccgcaccgg
      361 cgcgatgcgg cgcggctaca tggccttcgt atgggacctg ccgaaccgcc agcgcggcac
      421 cgtcgatgcg ccgatcgacc ggcatcccta cgcccgggag aaaatggcgg tgcgcgagag
      481 cggccgcgag gccatcaccc actgggaaat tcaggaatcc ttcaacgggc gcgacgccaa
      541 gccggtcgcg gccctgctgg cctgccagct cgagaccggc cgcacccatc agatccgggt
      601 gcatctcgcc catatcggcc atcccttgct gggcgacggc gtctatggcc cgcatttcaa
      661 gaccaaggcc agccatcttg gcccggaaag ccagaaggcg ctggccgccc tcggacgcca
      721 agcgctgcac gcataccttc tggcgctgga acacccccga aacggggccg ttttggagtg
      781 gatttcggat ttgcccgaag atctcgcttg cttgcgggat tcgctacggg cgacgcaatg
      841 acgcaggacc cctgaaaaag cgctgttatg ccaacaggtt atagcagccc cacggagacg
      901 tgacccccgc catccttcca aatgcccccc ggtttagtgt atgttgcacc tgcgttaaat
      961 atccaacgcg gaacatcgca gcccgggcgg ctttaacaca gcgtccgtct gccgggcccg
     1021 ccgctatcgg gggcctgaac cactggaggg cgctagcaaa tggcccgtac agctaccctg
     1081 ccgattctca acggagagtc cggcttatct cgttacctca gcgaaatccg caaattcccg
     1141 atgctggagc cgcagcagga atacatgttc gccaagcgct ggcgcgagca tgacgatcgc
     1201 gacgcggcgc accaccttgt caccagccat ctccggctcg tggccaagat agccatgggc
     1261 tatcgcggct acggattgcc gatctccgag gtcgtctcgg aaggcaatgt cggcctgatg
     1321 caggcggtga agcggttcga gcccgaaaag ggtttccgtc tcgctaccta cgccatgtgg
     1381 tggatcaagg cgtcgataca agagtacatc ctgcgttcgt ggtcgctcgt gaagatgggc
     1441 accaccgcga accagaagaa actgttcttc aacctgcgca aggccaagag caagatctcc
     1501 gcgctggacg agggtgatct tcatcccgac caggtgaagc tgattgccaa gcggctcggc
     1561 gtgacggacc aggacgtggt cgacatgaac cgccgtctcg gcggcgatgc ctcgctcaac
     1621 gcgccgatcc gtgacgacgg cgaagccggc gaatggcagg actggctggt cgatacctcg
     1681 cccaaccagg aagccatcat ggccgagcac gaggagttcg atcatcgccg cgaggcgctg
     1741 aacggcgcga tcggcgtgct caaccccagg gaacgccgca tcttcgaggc gcggcgtctg
     1801 gcggaagacc cgatgacgct ggaagacctc gccgccgaat tcggcgtgtc gcgcgaacgc
     1861 gtgcggcaga tcgaggtccg cgctttcgag aaggtgcagt cggccgtgaa aggcacgatt
     1921 gcccggcagg aagaagccac gctccacgcc gcgcattgat tttcgcggta aggcaatgag
     1981 agacaaaagc cggcggcaac gccggctttt ttgttgaggc gagagcagac gatgatctcg
     2041 catggggctg gagccgtcgc aattcgtgat ctccaaggac cgcgcgcgcc ggccacggtg
     2101 ccggtgatcg ggccgttctt atgcgactac acggtgttcg attatttttt tgccagcgcg
     2161 gaggatgcgc cgccgaaaac ctgcaatacg atctggcgat attcttcccg gccagcaatc
     2221 ggccgaccga gctgcagatg ttctgat
//
LOCUS       DASWGM010000321         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_991506,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000321 DASWGM010000000
VERSION     DASWGM010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991506"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1527)
                     /locus_tag="VGA15_09130"
     CDS             complement(241..1527)
                     /locus_tag="VGA15_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX9587895.1"
                     /translation="MTVSGTFSFDTPGDAARAAELRRVKALATLVLASTLAVFITAKA
                     LLHLHPAFGFVAAFAEAATIGGLADWYAVVALFRRPLGLPIPHTAIIQGNQHRIADKL
                     GEFIEVHFLEAAPVDVKLRQIDFATFIADWLRDRKRSTDLARFTLRLLPEAFSATETS
                     GLMTFITRRITTQLQSIDLAPLAAGTLRAFVAEGRHQGLLDDILRGVHQALTQPETLA
                     MIRAKIRAELPTLLKLYRTDKFLVNKIVASATAFFEEVRGDPKHPFRGEFDRMLLSFV
                     DRLGSDPSYADRIDGLKRELLARPELGDLARNIWSNARSFIERSASGETQVLQQHLAR
                     MFMGAGEALAGDSELRGEINQGLVTVLRSLIADQKSGVSSFISDQVKAWDMGQLISLI
                     EINIGKDLQYIRFNGSLIGGLAGLGLYTIEVLLRLL"
     gene            complement(1803..2285)
                     /locus_tag="VGA15_09135"
     CDS             complement(1803..2285)
                     /locus_tag="VGA15_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HEX9587896.1"
                     /translation="MTTDTNSVLNSVKEAFAPVTDALKNLQNLEVPEAARDFVKKTAG
                     TAKERAADLHAGTEKVTDAIETAVAGSVSEAAKISRNIQQAIYQDTEAFFAGIDKLAS
                     AKSLNEAVQIQSDMVRARGEVFMSRAKSTTEYLGKLVADGAKTAQDNLSKVATYGKTA
                     "
     gene            complement(2466..2843)
                     /locus_tag="VGA15_09140"
     CDS             complement(2466..2843)
                     /locus_tag="VGA15_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX9587897.1"
                     /translation="MTQKLTVWYNTKCPVCNAGIDWQQHRLVRAARSGAIDFRDINLE
                     PDALSGFRLGVNDVRRRLHAVDADNRLYAGIDCAIAIWLRTPGDIWLGRLVGLPVIRP
                     VAGFAYDRFADLLYAWNRWKGRW"
     gene            complement(2917..>3507)
                     /locus_tag="VGA15_09145"
     CDS             complement(2917..>3507)
                     /locus_tag="VGA15_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9587898.1"
                     /translation="SEMRSYPLHIATEAGDIEFRMMGPADEAAVLDFARKLPGHDLLF
                     LPRDISEPKVLAAWIKEIERGSIISLLAVKSGTVVGCGTIVRDPLSWSPHVGEIRMVV
                     STDVRGLGVGRALSQETFALALGIGLEKLLVQMTVDQTGAIAIFEGLGFKAEALLHDH
                     VRDLTGKKHDIVVLAHNVAQVRAQMEAYGLPDAVQH"
BASE COUNT          682 a         1086 c         1080 g          659 t
ORIGIN      
        1 agggttttgg acggcgggcg cagcgtctgg gcagtggcga acattgtagc tcctttcaga
       61 gccggcccct taagtttcgg cggccggcta atggaccata ggaagacgta aacctcatca
      121 aatcgttccg cgatgcaaca acctgtagtc aaagctaggg aaccgctttt gacctttcaa
      181 gctgctcatg ttgacgcggc cagggacgcc gcgtttagaa gccatgccgt gattaaatag
      241 tcacagcagc cgcagcaaaa cttcgatcgt gtagaggccc aatcctgcaa ggccgccgat
      301 cagcgagccg ttgaagcgga tgtattgcag atccttgccg atgttgattt cgatcagcga
      361 aatcagctgc cccatatccc aggccttgac ctggtcggag atgaagctcg agactccgct
      421 cttctgatcg gcgatcaagc ttcgcagcac ggtcaccaga ccctggttga tctcgccccg
      481 caactccgaa tcccctgcaa gcgcctcgcc cgcgcccatg aacattcggg cgagatgttg
      541 ctgcagcacc tgggtttcgc cgctggcgct gcgttcgata aacgatcgtg cgttcgacca
      601 gatgttgcgc gcgaggtcgc cgagttcggg gcgcgccaag agctcgcgct tcaatccatc
      661 gatccgatcg gcataggacg ggtcgctgcc gagccggtcc acgaacgaga gcagcatgcg
      721 atcgaactcg ccgcggaacg gatgctttgg gtcgccgcga acctcctcga agaacgcggt
      781 cgcggacgcc acgatcttgt tcaccaggaa cttgtcggtg cgatagagtt ttaacagcgt
      841 cggcaattca gcgcggattt ttgcgcggat catcgccagc gtctccggct gggtcagcgc
      901 ctgatgtacc ccacgcagga tgtcatcgag taaaccctgg tgccgcccct ccgccacgaa
      961 cgcacgaagc gtgccggcgg caagcggcgc gagatcgatc gactgcaact gggtggtgat
     1021 gcggcgggtg atgaacgtca tcaggcccga cgtttccgtc gccgaaaacg cctcgggcaa
     1081 aaggcgtagc gtgaaacggg cgagatccgt gctgcgcttg cggtcacgca gccagtcggc
     1141 gatgaaagtg gcaaaatcga tctggcgcag cttgacgtcg accggcgcgg cctcgagaaa
     1201 atgcacctcg atgaattcgc cgagcttgtc ggcgatgcgg tgctgattgc cctggatgat
     1261 cgcggtatgc gggatcggca atcccagcgg ccggcggaac agcgccacca ccgcgtacca
     1321 gtcggcgagg cccccgatgg tcgcggcttc cgcgaacgcc gcgacgaagc cgaaggccgg
     1381 atgcaggtgc agcaacgcct tggctgtgat gaacaccgcg agcgtgctcg ccagcaccag
     1441 cgtagccagc gccttgacgc gccgcaactc ggccgcgcgc gcggcgtcgc cgggcgtgtc
     1501 gaacgagaat gtgccggaga cggtcatggc ggctttcgga acggatggaa tctgtacata
     1561 ggggctgtgc acaccgtcgt catcctgacg cgagcaatgc atcgtgtccc ggacgcggtg
     1621 cggcgttctt cgcgccgctc cgcagagccg ggacccacgc gcaagtgata gaccccggct
     1681 ctgctgcgca ccgctacgcg ctgcgcagcg tccggggaac gtaacccgcc catcaaaaag
     1741 aaggcccgca tgaagcgggc ctctaaatga tctgccgatc gactgatcct aaagcaaagc
     1801 gatcaggcgg tcttgccgta agtcgcgacc ttggacaggt tgtcctgcgc ggtcttcgca
     1861 ccgtcggcga cgagcttgcc gagatattcg gtggtggact tggcccgcga catgaacact
     1921 tcgccgcgcg cacggaccat atcggactgg atctgaaccg cctcattgag cgacttggcg
     1981 gaagccagct tgtcgatgcc tgcgaagaag gcttcggtgt cctgataaat cgcctgctga
     2041 atgttgcggc tgatcttggc ggcctcagag accgaaccgg caacggcggt ttcgatcgcg
     2101 tcggtcacct tctcggtacc ggcgtgcagg tcggcagcac gctctttggc ggtgccggca
     2161 gtcttcttga cgaagtcacg ggcagcctcg ggaacttcca ggttttgaag attcttgagc
     2221 gcgtcggtga cgggtgcgaa ggcttccttg acggaattca gcacggaatt ggtatcggtg
     2281 gtcattgggt ttctccatcc tcaggttttg agctatgggc tcaccccgtc cggattggcc
     2341 cggggcgaga tggttgtgcc atagttaatg gtgcatcgca acatcaatgt tgcggtgcga
     2401 tatcactaat ttgtgatcag ccaatgattt gagcggccca gcccaacaat caggctccct
     2461 tcgccctacc agcggccttt ccagcgattc cacgcgtaca acaaatcggc gaaccggtcg
     2521 tacgcgaagc cggcaaccgg ccggatcacc ggcaggccga cgagccgccc aagccagatg
     2581 tcgccgggcg ttcgcagcca aatcgcgatc gcgcagtcga ttccggcgta gaggcgattg
     2641 tcggcatcga cggcatgcag ccggcgcctg acgtcgttga ccccaaggcg gaagccagag
     2701 agcgcatccg gctcgagatt gatatcgcga aagtcgatcg cgccggatcg ggcggcgcga
     2761 accagccgat gctgctgcca gtcgatgccg gcgttacaca ccggacattt ggtgttgtac
     2821 caaaccgtga gtttttgggt catcgcaccg gcacattcga gacgcgccga tcatgatccg
     2881 aatatgtgac cttcttgcgc ctgaccgccc ggcgacctag tgctgcacgg catccggcag
     2941 cccataggct tccatctggg cccgcacctg ggcgacgtta tgcgcgagca cgacaatgtc
     3001 gtgctttttg ccggtcaggt cgcggacgtg gtcgtgcagc agcgcttcgg ccttgaatcc
     3061 gaggccttcg aagatcgcga tggcgccagt ttgatcgacc gtcatctgaa ccagcagctt
     3121 ttccagcccg atgcccaaag cgagcgcaaa agtttcctgg gacagcgccc gcccaacgcc
     3181 gagaccgcgg acgtcggtgg acaccaccat ccggatttca ccgacatgcg gcgaccatga
     3241 cagcggatcg cgcacgatgg ttccgcatcc gaccaccgtt cccgatttca ccgccaacag
     3301 gctgatgatc gaaccgcgct cgatctcctt gatccaggcg gccagcacct tcggctcgct
     3361 gatgtcgcgc ggcaggaaca acaaatcatg gcccggaagt tttcgcgcga agtcgagcac
     3421 cgccgcctcg tcggccggtc ccatcatccg aaactcgatg tcgccggcct cggtggcaat
     3481 atgcagcgga tagctgcgca tttcgct
//
LOCUS       DASWGM010000322        16707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_159361,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000322 DASWGM010000000
VERSION     DASWGM010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16707
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159361"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VGA15_09150"
     CDS             <1..186
                     /locus_tag="VGA15_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEX9587899.1"
                     /translation="FMSAAETNDNPQPLEIRQNIANIGVFVMKQTVDMQMNPDPAKLK
                     SLIDINCNLAAGLSGRV"
     gene            213..578
                     /locus_tag="VGA15_09155"
     CDS             213..578
                     /locus_tag="VGA15_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587900.1"
                     /translation="MADIMSTLSANYKTVGLTVPSKTRYVAVDPALYQGSWTGKYANN
                     KSFTITVSDVSGFHAKVKYQSDSTVKYQDVLIKDLSFRVGDTKFTLTKKLGTAQIKNV
                     VTDPATGSTYLDTATATRS"
     gene            complement(581..946)
                     /locus_tag="VGA15_09160"
     CDS             complement(581..946)
                     /locus_tag="VGA15_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587901.1"
                     /translation="MSTDFSIRPVGAPVATPIAPPVSEAANSAVATELPASQSVTVAD
                     ASAAARNDSYAVNISISRQVILDQAAASIVYQVVDTKTDTVVTQFPDEAVLRRRAYFR
                     ALDLTKDAPARVLATDRRA"
     gene            complement(1112..1597)
                     /locus_tag="VGA15_09165"
     CDS             complement(1112..1597)
                     /locus_tag="VGA15_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587902.1"
                     /translation="MTKHPPSQPAPIPVASTPGEARKLAENLMEVMSALLGVIERETE
                     LVRAGKVREAMALGPNKTDFSRRYVGAIAHLKASQKYLSQVAPDLLATLHRHHDVFRA
                     MLQINLTVLATAHAVSEGIVRGVNTEMQRRNIPNTYTSTGARAVPGPRHMTPLAVSRS
                     L"
     gene            complement(1615..1938)
                     /gene="flgJ"
                     /locus_tag="VGA15_09170"
     CDS             complement(1615..1938)
                     /gene="flgJ"
                     /locus_tag="VGA15_09170"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEX9587903.1"
                     /translation="MPAKAMMPLLNGRPDYQLADALTKVSPQAQAKAKGTATDFEAMF
                     LNSMFSQMTSGIKGEGPFGDTPGTGVWRSMLTEQYSKSFAKAGGVGISNDVYRTLILQ
                     QANHS"
     gene            complement(1974..3098)
                     /locus_tag="VGA15_09175"
     CDS             complement(1974..3098)
                     /locus_tag="VGA15_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX9587904.1"
                     /translation="MPSVYSARVLQVAGAALLALALSALPAGATSRIKDLANIEGVRQ
                     NQLIGYGLVVGLNGTGDTLNNIPFTKQSLQAMLERMGVNIRGATIRTGNVAAVMVTGN
                     LPAFGTQGTRMDVTVSALGDSKNLQGGTLLVTPLLGADGNVYAVAQGSVAVAGFAAEG
                     AAASVVRGVPTVGRIANGAIIEREIEFALNRLPNVRLALRNADFTTAKRIAAAVNDFL
                     GVKTAEPIDPSTVQLSIPAEFKGNVVAFLTEIEQLQVEPDLAAKIVIDERSGIIVMGR
                     DVRVATVAVAQGNLTVTISESPQVSQPNPLSRGRTTVTPNSRVGVTEDGKKFALVKDG
                     VSLQQLVDGLNGLGIGPRDLIGILQAIKAAGAIQADIEVM"
     gene            3363..3779
                     /locus_tag="VGA15_09180"
     CDS             3363..3779
                     /locus_tag="VGA15_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HEX9587905.1"
                     /translation="MRIYGPNGTTLGTPSANVRRTSSTGFSLPDAAAATQESRAAVAP
                     KAAANIDALLALQGIEEDPAERRKRSVQRGKGALDVLDDLKIALLSGNFDASTMSRLR
                     DAAANLKSSSGDPGLDSVLSEIELRVEVELAKAGQF"
     gene            4007..4417
                     /gene="dksA"
                     /locus_tag="VGA15_09185"
     CDS             4007..4417
                     /gene="dksA"
                     /locus_tag="VGA15_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX9587906.1"
                     /translation="MEKLKSYRPSEKEPFMSDRQRDYFRAKLLAWKDEILRESRITLQ
                     TLQEENVNHPDLADRASSETDRAIELRARDRQRKLISKIDAALQRIDDNTYGYCEETG
                     EPIALKRLEARPIATLSVEAQERHEKREKVYRDE"
     gene            4686..5471
                     /locus_tag="VGA15_09190"
     CDS             4686..5471
                     /locus_tag="VGA15_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9587907.1"
                     /translation="MNDTFAAAEAIAAARRNRTPLHPLAGDSAPQTEAEGYRIADAVH
                     GLLSRDFGEMVGHKIGCTSAVMQQYLNIPHPCSGGVFANGVHPSGAALRAKDFIRVGV
                     ECEIAVRLARDLMPLDAPFTADKVVQAIDAYLPAIEIVDDRYSDWQTLGAPTLVADDF
                     FAAGCVLGNPVARSAAPDLLEVIGRALINGVEVGQGTGADVLGHPHHALAWLANHLAS
                     DGNGLSAGEIVLTGSLVKTIWLTAGDSVVMELSGLGSVAATFS"
     gene            complement(6198..6710)
                     /locus_tag="VGA15_09195"
     CDS             complement(6198..6710)
                     /locus_tag="VGA15_09195"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587908.1"
                     /translation="MGLSAGDELNIVDVVERTLVVQKQDRRKAALGTPGLPQLDASPR
                     LQIRSGRGQRAVKAFFDTDIRERANRPSTAPRMLRSALGSAHRAARAQAPRCAADPGP
                     TLPAPIWVPALRSSAKSAAPRPGHGWSMLSPPFGDAPAGRFVVTFQADLGSPVLFAKI
                     FRFPLPPNHL"
     gene            6597..7166
                     /locus_tag="VGA15_09200"
     CDS             6597..7166
                     /locus_tag="VGA15_09200"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9587909.1"
                     /translation="MREARRSERSLAPVLLLYDQRSFDHIDDVEFVARAQAHLLDQRF
                     GQPHGQAVAPFQNLHGKLHSLSRDDIISLPRELRKRREEMSNPKKVTASSGNVFADLG
                     FDNPEEQLLKAKLVREIRGIIKRRKLTQTKAAAMLGLKQPDVSALVTGRVGKFSIDRL
                     VRCLDRMDYKVDVVIRHKPRRASSRAVAA"
     gene            7493..8854
                     /locus_tag="VGA15_09205"
     CDS             7493..8854
                     /locus_tag="VGA15_09205"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEX9587910.1"
                     /translation="MPDKNDFDRYLLALRKTKIDEKTEHTDRAALQVLLQAFANESSN
                     RTTVQHEPKRVADKGAPDFKITSRGLILGYVENKAIGEHLEKVLKSNQIARYKTLSQN
                     IILTDYLHFIWLSKDGIQRETLCHATDLENPGFRLHEDRIAAVSVLLQAFFSTAPEGI
                     GRAEQLALALATRSKLLHDYLGEELVRQEREHKEGRLYGLFQIFRDQVFHELTLNEFA
                     DAFAQMLAYGLFLARLNSGLEAVTLHNAREHVPGSFRLIRELVDFLTELEKFEYRDVR
                     WVVEEVLSIVNALNLPAIHEDLSFRHRKAISRKVRAGDEEEHRLFERDPFIYFYEDYL
                     KAYDATARKARGVYYTPPPVVNFIVRAVDDILKQSFGIRDGLADHTRVTVLDFACGTG
                     TFLLEVCQRIFDNIGGPDVGSADLIVRDHLLKNLFGFEYLIAPYTIAHLKLSQYLRDR
                     GIR"
     gene            8902..10629
                     /locus_tag="VGA15_09210"
     CDS             8902..10629
                     /locus_tag="VGA15_09210"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEX9587911.1"
                     /translation="MEPQANLLLPAISAEVQAAQKVKDQPILVITGNPPYAARSKNRG
                     KWIKKQIKAYEIVDGEHFGERKHWLNDDYVKFLRFAQLKIEARGDGVVGVITNRWWLE
                     NVTFRGMRQSLLNTFDQIHIFDLLGEAGQKDDKNVFDITKGVAIALLVRNSQAEPRIR
                     YASIRGTRLQKYKAAADSSLNDLDWTDLNPSSPNYFLFPRTEVGRELYESFWSVGKIF
                     EEGSTGVLTGRDRLSVAETDGALRDHLEALEGSAVDREIETRFNLDQVEHWSLSDTRQ
                     LIREEGIRRNLIRKLAYRPFDTQYYYDHDALVFRRRHKVMQQMTGGQVGLAVCRLTKG
                     GDWRHCMIAGDPSDDSYVSDKSKERAYLYPLYFTQQPVRESKNAVVRESSINVEPPKN
                     ENLSSDFRAFLDSRYDHHYTPEEILGYIYAVLHAPAYRLLYAEFLSIDFPRVPFPESS
                     EEFETLSGLGWALVQAHLLRELPRQGLAVYHGKGDHVVETVRYSPEERAIAINKTQSF
                     KSVPETVWEFRVGSHQVLDKYLKSRKGRTLSLDEINHVAAIVDSLAFTIEQMAKIDEA
                     YASAFPNRG"
     gene            10704..10988
                     /locus_tag="VGA15_09215"
     CDS             10704..10988
                     /locus_tag="VGA15_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587912.1"
                     /translation="MARDHQPDMFSESTQSDLFGAEPIPAYRPDPEKVRARLHKILAE
                     ARAARTFPWEPTRVSLYRTIFPQMSLWLPEDESAQLRFEFEAEIKRLEAA"
     gene            complement(11298..11675)
                     /locus_tag="VGA15_09220"
     CDS             complement(11298..11675)
                     /locus_tag="VGA15_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587913.1"
                     /translation="MRITSCAIAAAAVFAATAASAQTVNLTGMYRCIQGCRAAPVGSV
                     AYVTQNGWNLNLVNEAGQPSRAWPDAFAPMTKIWADDWNVSAVYSPDGMTIQFDNGTI
                     WQRDMGPPPPLRVVRVRRQAALR"
     gene            complement(11988..12743)
                     /gene="flgH"
                     /locus_tag="VGA15_09225"
     CDS             complement(11988..12743)
                     /gene="flgH"
                     /locus_tag="VGA15_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX9587914.1"
                     /translation="MYRSGFNRLLVNGTLLVILSVASGCSSIDRLSQIGEKPKLTEIE
                     NPTTQPGYKPVQMPMPKPETASYNANSLWRNGSRAFFKDQRAARIGDILTVTVNITDK
                     ANIANETQRSRTAKDDSGLTDFAGSKLLGGDAAKVLPGRLLTADSTSSSDGKGSVNRQ
                     EALQTNVAAVVTQLLPNGNLVVEGKQEIRVNFEIRELIVAGIVRPEDIQSDNTIDSSK
                     IAQARIAYGGRGQITDVQQPRYGQQVMDVLLPF"
     gene            complement(12749..13873)
                     /gene="flgA"
                     /locus_tag="VGA15_09230"
     CDS             complement(12749..13873)
                     /gene="flgA"
                     /locus_tag="VGA15_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEX9587915.1"
                     /translation="MTLRSLLLATALLAAASTAASAQVSDDTIAAPVLRASVNVTADV
                     VRIGDVIDNAGTSAQIAIYRAPDLGTTGSLKVASVLSALQAHQVIGVDTRDLKEISVT
                     RLARTLESKDIELQVARALEHRGGLGEAANLSLTFDRDVQDLRLDASNSGAMQATSAR
                     FDPRSGRFDVSFEIANDNVSTPTRLRFSGSAIETVEAAVLARGIERNEVIKSSDVVVE
                     RRPKAEVGGDVASRDRAIGMQARKQLRAGQALRVADIAKPDLVQRDQNVTLTYEATGL
                     YLTIRGKALDNGTEGDVVSVLNLQSKRTVTGVVSGRGQVTVSAPAARLPPAADATSSI
                     GSSEVAAPVSVAVNSTSQVTQQVTQQAAQQVAQHAAPKAE"
     gene            complement(13886..14674)
                     /gene="flgG"
                     /locus_tag="VGA15_09235"
     CDS             complement(13886..14674)
                     /gene="flgG"
                     /locus_tag="VGA15_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX9587916.1"
                     /translation="MRALYTAATGMAAQELNVQVISNNIANMRTTGFKKQRAAFQDLI
                     YDHVRRVGAAASDQGTILPVGVDIGGGVKTVGTPRLMSQGTLAQTGNDLDIAIRGEGF
                     FKIQMPDGSFTYTRDGSFQMDAQGRVVNAQGNPVQPTITIPQNASGIAINAQGQLSVT
                     LPGSTTPTTVGQIGLTRFINKAGLQPIGDNLFTDTPASGPPQDGIANTDGFGDMQQSN
                     LEQANVEVVTEISDLIAAQRAYEMNAKVISAADQMLQSASALFR"
     gene            complement(14687..15451)
                     /gene="flgF"
                     /locus_tag="VGA15_09240"
     CDS             complement(14687..15451)
                     /gene="flgF"
                     /locus_tag="VGA15_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEX9587917.1"
                     /translation="MENMLLVGLSRQMTLERQMDVVANNVANVNTNGYKADRSLFSEY
                     LNSGAHEDNFVGRDRAVSYVLDNGTFKDFTQGPTEQTKNPLDVAIDGGGFLVVQTRAG
                     ERYTRDGGLQINNQGQLVTAAGDPVLGTSGPIVFQPTDHDINIAADGNVTVVEGVGHT
                     DSVRGKLRLVSFAQAQRLLKEGSNLYAAGEGGAPQPDTASKVRQGFVEKSNVNSVAEM
                     SRMIEVTRTYTQIATMLQTQSDLHKSAIEKLADVPA"
     gene            15814..16308
                     /gene="fliL"
                     /locus_tag="VGA15_09245"
     CDS             15814..16308
                     /gene="fliL"
                     /locus_tag="VGA15_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HEX9587918.1"
                     /translation="MSDTDSADGGAAEPVGAPKGKLKLIIAAVGLFVILGGGAATWFM
                     FFRGHSEEVHAEAAAAPKPAIYIDVPEMLVNLVGAPGDRVQYLKARIVLEVKEEKQVE
                     AIKPTMPRVTDIFQTYLRELRPADLTGSAGLFRLKEELTKRVNTAISPSQVNAVLFKE
                     IVVQ"
     gene            16328..>16707
                     /gene="fliM"
                     /locus_tag="VGA15_09250"
     CDS             16328..>16707
                     /gene="fliM"
                     /locus_tag="VGA15_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642814.1"
                     /note="with FliG and FliN makes up the switch complex
                     which is involved in switching the direction of the
                     flagella rotation; Bradyrhizobium has one thick flagellum
                     and several thin flagella; the protein in this cluster is
                     associated with the thick flagellum; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX9587919.1"
                     /translation="MAGNSIDDQDAIAAQWEASLDSEDPAEAAAAAAANELSGTMALQ
                     WAAMVEDGSRDFGGGKNGGERVLSQEEIDNLLGFNVGEVNLDDNSGIRAIIDSAMVSY
                     ERLPMLEIVFDRLVRLMTTSLRNFT"
BASE COUNT         3210 a         4992 c         5109 g         3396 t
ORIGIN      
        1 ttcatgagcg cggcggaaac caacgacaat ccgcagccgc tggaaattcg tcagaacatc
       61 gccaatatcg gggtgttcgt gatgaagcag acggtcgaca tgcagatgaa tccggatccg
      121 gcgaagctga aatcgctgat cgacatcaat tgcaatctcg cggcgggcct gtcgggccgc
      181 gtttgatggc ttgcggctag cggagggcta ccatggctga cattatgagt accctgtctg
      241 cgaactacaa gaccgtggga ttgaccgttc caagcaagac ccggtatgtc gcggtcgatc
      301 ccgcgctcta tcagggcagc tggaccggaa aatacgccaa caacaaatcg ttcacgatca
      361 cggtctcgga cgtcagcggg tttcacgcca aggtcaaata tcagagtgat agtaccgtca
      421 agtaccagga cgtcctgatc aaggatctgt cgtttcgggt tggcgatacg aaatttacgc
      481 tgaccaagaa gctcggaacc gcgcaaatca agaacgttgt cacggatcca gcgaccggct
      541 caacctatct cgatacagcc accgcgacac ggagctgatt tcaggccctg cggtcggtgg
      601 cgagaacgcg ggcgggggcg tccttggtca gatcgagggc gcggaaatag gcgcggcggc
      661 gcagcaccgc ttcatcggga aactgcgtta ccaccgtgtc ggtcttggta tcgacgacct
      721 gatagacgat cgaagccgcg gcttgatcga ggatcacctg acgcgatatc gaaatgttca
      781 cggcatagga atcgttgcgc gcggcagcgc tggcgtccgc aaccgtgacg ctctggctcg
      841 ccggcagctc ggtcgcgacc gcgctattcg ccgcttcgct cacgggagga gcgatcggcg
      901 tggcgaccgg tgctcccacc ggtctgatgc tgaaatccgt actcatggca gcctcctggc
      961 ttccctcgag tcaaatccca acccctctcc catcgcaact atgcgcttca cctctcaacg
     1021 tggtgttaga gaatgcactg aatatcgcgc tgatgccggc gcgatagttt gattaaaatt
     1081 gtcgccattc aaacccgcgc cgcggcgatt ttcacagcga acggctgacc gccagcggcg
     1141 tcatgtgccg cggtcccggc accgcgcgcg ctccggtcga cgtgtaggtg ttgggaatat
     1201 tgcggcgctg catctcggtg ttgacgccgc gcacgatgcc ttccgacacc gcatgcgcgg
     1261 tcgcgagcac cgtgaggttg atctgcagca tggcgcgaaa cacgtcatgg tgacgatgca
     1321 gcgtggccaa cagatccggc gccacttggc tgagatattt ctggctggcc ttcagatggg
     1381 cgatcgcgcc gacatagcgg cgggaaaaat cggtcttgtt cggcccgagc gccatcgcct
     1441 cgcgcacctt gccggcgcga accagttcgg tctcgcgctc gatgacgccg agcagcgcgc
     1501 tcatgacctc catcaggttt tcggcgagct tgcgggcctc gccgggcgtc gaggcgaccg
     1561 ggatcggcgc gggctgcgat ggaggatgct tggtcatgtc tggttttccc ggatctagct
     1621 gtggttggcc tgctgcagaa tcagcgtgcg atagacgtcg ttggaaatgc cgacgccgcc
     1681 ggccttggcg aaggatttcg aatattgttc ggtcagcatc gaacgccaca ccccggtgcc
     1741 cggggtatcg ccgaacgggc cttcgccctt gatgccgctg gtcatctgcg aaaacatcga
     1801 gttgagaaac atcgcctcga aatcggtcgc cgtgcccttg gccttggctt gcgcctgcgg
     1861 agataccttg gtcagcgcat cggcgagctg ataatccggc cggccgttga gcagcggcat
     1921 catggctttg gcgggcatgc tgccgtgggg gtggttggtc agaccgctct gcatcacatc
     1981 acctcgatgt cggcctggat cgcgccggcg gccttgatcg cctgcaggat gccgatcagg
     2041 tcgcgcgggc cgatgccgag cccgttgagg ccgtcgacca actgctgcag cgatacgccg
     2101 tctttcacca gcgcgaattt cttgccgtcc tcggtgaccc cgacgcgtga gttcggcgtc
     2161 accgtggttc gtccgcgcga cagcggattg ggctggctga cctgcgggct ctcggaaatc
     2221 gtgacggtga gattgccttg cgcgaccgcg acggtggcga cgcggacatc gcggcccatc
     2281 acgataatgc cggagcgctc gtcgatcacg atcttggcgg cgaggtccgg ctcgacctgc
     2341 agctgctcga tctcggtcag gaaggcgacc acgttgccct tgaattccgc cgggatcgac
     2401 aactgcacgg tggagggatc gatcggttcg gcggtcttga cgccgagaaa atcgttgacc
     2461 gcggccgcga tgcgcttggc ggtggtgaaa tcggcattgc gcagcgccag ccgcacattg
     2521 ggcaggcggt tcagcgcgaa ctcgatctcg cgttcgatga tggcgccatt ggcgatgcgg
     2581 ccgacggtcg gcacgccgcg gaccacgctg gcggcggcac cttcggccgc aaagccggcg
     2641 accgcgaccg aaccctgcgc cactgcatag acgttgccgt cggcgccgag caggggggtg
     2701 acgagcaggg tgccgccctg cagattcttg gaatcgccga gcgcggacac cgtgacatcc
     2761 attcttgtgc cctgggtgcc gaacgccggc aggttgccgg tgaccatcac ggcggcgacg
     2821 ttgccggtgc ggatggtggc gccgcggata ttgacgccca tccgctccag catcgcctgc
     2881 agcgattgct tggtgaaggg gatgttgttc aaggtgtcgc cggtgccgtt gaggccgacg
     2941 acgaggccat agccgatcag ctggttctgc cgcacgcctt cgatgttggc gaggtccttg
     3001 atccgcgagg tcgcgccggc aggcagtgcc gacagcgcca gcgccaacag cgcggcaccg
     3061 gccacttgca acactcttgc cgaatagacg cttggcatcc tctgctcccc gctgaggttt
     3121 tgggctggac ctgcgcatca ctcccatgac ggcgttccgt ccatccctat gcgagcctcg
     3181 tgccatgcca tttccttcac ttaattcatt gatagtgtta tctaaattta gaaagcttgg
     3241 tcgtgcttcc gctgctcccg ggcggcgaaa ctgccgtcgg cggcagattt tgccgggtcg
     3301 tttacactcc gttaaccaca gcggggcaaa gctgccggtg acacaccccg cgagatccga
     3361 cgatgcgcat ctatggaccc aacggcacca cgctcggcac gccctcggcc aacgtccggc
     3421 gaactagctc gaccggcttt tcgctgccgg acgccgccgc cgccacccag gaaagccgcg
     3481 cggcggtcgc gcccaaggcg gcagccaaca tcgatgcgct gctggctttg cagggcatcg
     3541 aggaggatcc ggccgagcgc cgcaagcgct cggtgcagcg cggcaagggc gcgctcgacg
     3601 tgctggacga cctcaagatc gcgctgttgt ccggcaattt cgacgcttcc acgatgagcc
     3661 ggttgcggga tgccgccgcc aacctgaaat cctcctccgg cgaccccggc cttgattcgg
     3721 tcttgtccga gatcgagctg cgggtcgaag tcgaactggc caaagccggg cagttctgag
     3781 ccggatcgtt ctgacagcct ttgggcgatc ctttgggcaa tccttgagca ttgctttggg
     3841 caattctttg ggcttccttg gggcttcgcc acgggttccg gccgggaaac cttcgcggtg
     3901 tagcgtttcc cggttgcagg cgatattgtc tgtcacaaca caccgctata taaggcgcgc
     3961 gcggccgacg cttttcggac cgccttgcat cacagacggg ctgaacttgg aaaagttgaa
     4021 aagctatcga ccctccgaaa aagagccttt catgagcgat cgccagcgcg attattttcg
     4081 cgccaagctg ctggcatgga aggacgagat cctgcgcgaa tcgcggatca ccctgcagac
     4141 cctgcaggaa gagaacgtca atcatcccga tctcgccgat cgcgcatcgt ccgagaccga
     4201 ccgcgcgatc gaactgcgcg cccgcgaccg gcaacgcaag ctgatctcca agatcgacgc
     4261 ggcgctgcag cgcatcgacg acaacaccta cggctattgc gaggagaccg gcgagccgat
     4321 cgcgctgaag cggctggaag cccgcccgat cgcgacgctg tcggtggaag cgcaggagcg
     4381 ccacgaaaaa cgcgaaaagg tttatcgcga cgaataaggt ctgaccgcca cgtgcggtca
     4441 ttttgctgat tccctcctca tagtgagggg cggcaatcga tttcatcgtc atggccgggc
     4501 ttgtcccggc catccacgtc ttgacggcgt agcagcaaga aagacgtgga tggccgggac
     4561 gaagcctggc catgacgaaa aaacgaatcc ttttcaagtc gttagggggc atcaggaatg
     4621 ctcgctctca ggatcaggtg cgtcgtgatg caagcccttt catcaaacac cggaggctgc
     4681 cgccgatgaa cgacactttc gcggcggccg aagccatcgc cgcagcgagg cgcaatcgta
     4741 cgccgctgca tccgcttgcc ggcgatagcg cgccgcagac cgaagccgaa ggctaccgga
     4801 ttgcggacgc cgtccacggc ctgctgtcgc gcgatttcgg cgagatggtc ggccacaaga
     4861 tcggctgcac cagcgcggtg atgcagcaat atctcaacat tccccatccc tgcagcggcg
     4921 gcgtgtttgc caacggcgtt catcccagcg gtgcggcgtt gcgcgcaaag gatttcatcc
     4981 gcgtcggcgt cgaatgcgaa atcgccgtcc ggctcgcccg cgatctgatg ccgctcgacg
     5041 cgccgttcac cgcggataag gtggtgcagg cgatcgacgc ctatctgccg gcgatcgaga
     5101 tcgtcgatga ccgctacagt gactggcaga ctctgggcgc gccgaccctg gtggcggacg
     5161 actttttcgc ggccggttgc gtgctcggca accccgtggc gcgatccgcc gcgcccgatc
     5221 tgcttgaagt gatcggccgc gcgctgatca acggcgtcga ggtcgggcag ggcaccggcg
     5281 ccgacgtgct cggccatccc caccacgccc tggcctggct cgccaatcat ctcgcctccg
     5341 acggcaacgg cctctccgca ggcgagatcg tgctgaccgg aagtctcgtc aagacaatct
     5401 ggctcaccgc cggcgacagc gtggtgatgg agctctccgg gctcggcagc gtggccgcga
     5461 cgttttcctg agcggggcca tgcaggtggc gcgctcgctt caccgtcatg gcgagcgaac
     5521 gggtcggcgt aaagaggctc cgcgtgcaat ccataaccgt cattccgggg cgatgcgaag
     5581 catcgaaccc ggaatctcga gattccgggc ctgcgcctga cggcgcatcc cggaatgacg
     5641 gagcctgtgg tgagtcgtca cttcgcttca caaagattac agcgcactcg tactctccaa
     5701 acacgcgtcc gcggcctcgc cgcgcgacgc gcccgagccg ttgatgaatc tctcgccccc
     5761 ttgagggcgt ggggaatgcc gggcgcccgt tgcacccgcg gcctcgtgtg cacttgtagt
     5821 gatagaacgc acacgagtaa caacgagtac accggcatca cccggcattc ccgcacgcaa
     5881 tggttttaac ggtttccttc gcgctctccc cggtgaccgg gctcttttgc caccgtcgcc
     5941 tgcggattaa gttttgtcta agcccggtag ggccgacgca actccgcaaa acttgacgcc
     6001 agcgtcgggg cgtcaggacc acacgacttc gccgtccgcg actgcatctc tcgtccgcgc
     6061 gctctttgat cgctcacaag tctttcgact caccctgcga tcccatcgcg cgcaaaacgc
     6121 tgccgcgtcc accgcatccc gccccgcgtc cgtgacgatc gcgagccgcc cctctgtggg
     6181 acgagacggg aaaagttcta gagatgattt ggggggagcg gaaagcggaa tatttttgca
     6241 aagaggactg gactgcccaa atcagcttga aaagtcacaa caaatcgacc tgcgggcgca
     6301 tcaccaaacg gcggcgaaag catactccac ccgtgtcccg gacgcggtgc agcgctcttc
     6361 gcgctgctcc gcagagccgg gacccatatc ggcgctggca aggtgggccc cggatcagca
     6421 gcgcaccgcg gagcctgtgc tcgggccgcg cgatgcgcgg acccgagtgc gctgcgcagc
     6481 atccggggag cggttgaagg gcggtttgcg cgttcacgaa tgtcggtatc gaaaaacgcc
     6541 ttcaccgctc gttggcctcg tcccgatcga atttgtaatc ggggggaagc gtccagttgc
     6601 gggaggccag gcgttccgag cgcagccttg cgccggtctt gcttctgtac gaccagcgtt
     6661 cgttcgacca catcgacgat gttgagttcg tcgcccgcgc tcaagcccat cttctcgacc
     6721 agcgcttcgg gcaaccgcac ggccaggcag tcgccccatt tcaaaacctg catggcaaac
     6781 tccatagttt gagccgagat gatatcatct cattaccgcg agaactacgg aagaggaggg
     6841 aagaaatgag caaccccaaa aaagtgaccg cgagcagcgg caatgttttt gccgaccttg
     6901 gttttgacaa cccggaagaa cagctgctca aggccaagct tgttcgcgaa atccgtggaa
     6961 ttatcaaacg ccggaagctt acccagacca aggctgctgc catgctcggt ctcaagcagc
     7021 cggacgtctc tgctcttgtt accggtcgcg tcggcaaatt ctcaatcgat cgtctggttc
     7081 ggtgcctgga ccgaatggac tacaaggtag atgtcgttat acgccacaag ccgcgccggg
     7141 cttcttcgcg ggccgttgcg gcctaaagca tgatccggaa aagtggaaac cggttttccg
     7201 aaaagatcaa gctcgaacaa aaagataacg ctagggtctg attcaactca gttgaatcag
     7261 accctagcgc cgtcgaagcg ggaatgattc gcgatcgccg ggaatttgtt tgcacgagac
     7321 ggcctacctg aaggcatcac ctttggcgag ccgtggcgcg ccctttcaga tgcgccagtc
     7381 gccatatctt acccgctctt caaacctaag aagtggttgt ggggcatgcg gctttcgagt
     7441 agcgattgcg gactaaaccc gcaactattg gatcgcggga tggaaacgcc tgatgcccga
     7501 taagaacgac ttcgatcggt acctccttgc actgcgcaag accaagatcg acgagaaaac
     7561 cgagcacaca gatcgcgcgg cattacaggt gctcttgcag gcctttgcta atgagagcag
     7621 caaccggacc acggttcagc acgaacccaa gcgcgtggcc gacaaaggtg ctccggattt
     7681 caagatcacg agccgcggcc tcattctcgg ttacgtcgag aacaaggcga ttggagagca
     7741 cctcgaaaag gtcctgaaat ccaatcagat cgcgcgctac aagactctct cacaaaacat
     7801 cattcttact gactacctcc acttcatctg gctaagcaag gacggcatcc agcgtgagac
     7861 gctgtgccat gccaccgacc tcgaaaatcc cggctttcgc ctgcatgaag atcgcatcgc
     7921 ggctgtatcc gtattattgc aggccttctt ttcaacagcg ccggagggca tcggccgtgc
     7981 ggagcagctt gctttggcgc tcgcgacgcg aagtaagctg ctgcacgatt atctcggcga
     8041 agaactagtg cggcaggagc gtgagcacaa ggaagggcgt ctttacggat tatttcagat
     8101 ttttcgggat caggtgttcc atgagctgac gctgaacgaa tttgcagatg ccttcgcgca
     8161 aatgctcgct tatggattgt ttctagcacg gctcaattca ggtttagaag cggtcacgct
     8221 acacaatgca cgcgaacatg tccctggctc gtttcgtctc attcgcgaat tggttgattt
     8281 tctcaccgaa cttgagaagt ttgaataccg cgatgtccgc tgggtggtcg aggaggtgtt
     8341 atcgatcgtc aatgcattga accttccggc aattcacgag gacttgtcat tccggcaccg
     8401 taaggcgatc agccgcaagg tgcgcgcagg cgatgaagaa gagcatcggc tgttcgagcg
     8461 cgatccattc atctattttt acgaggatta cctgaaggcc tatgacgcga cggcacgcaa
     8521 ggcccgtgga gtctactata caccgccgcc ggtcgtgaat ttcatcgtgc gcgcggtgga
     8581 tgacattctg aaacagagtt tcggcatccg cgacgggttg gccgaccata cgcgcgttac
     8641 tgtcctcgac tttgcttgtg ggactggaac attcctcttg gaggtgtgtc agcgtatttt
     8701 tgataacatc ggtgggccag atgtcgggag cgccgacctc atcgtccggg atcatctttt
     8761 gaagaatcta tttggcttcg agtatttgat agcgccgtac acgattgcgc acctgaagtt
     8821 gtcccagtat ctccgagaca ggggcatccg ttaaagagcg acgagcgtct gcagatcttt
     8881 ctgaccaaca cgctggagcc ggtggagccg caagcgaacc ttttgcttcc agcgatcagt
     8941 gctgaagtac aggctgcgca aaaggtaaag gatcaaccca ttttggttat aactggcaac
     9001 ccgccgtacg cggcgcgttc caaaaacagg ggcaagtgga taaaaaaaca gatcaaggcc
     9061 tatgagattg tggacggaga gcattttggt gaacgtaagc attggctcaa tgacgattac
     9121 gtgaaatttc ttcgctttgc tcaactaaaa attgaggccc gcggcgatgg cgttgttggg
     9181 gttatcacca atcgttggtg gcttgaaaat gttacgtttc gagggatgcg ccaatcccta
     9241 ctgaatacgt tcgatcaaat acacattttc gatttactgg gagaagctgg acagaaggac
     9301 gataaaaacg tctttgacat aacaaaaggg gtagcgatag cgcttcttgt tcgtaactcg
     9361 caggcagagc cgcgcataag atacgcctcg atccgcggaa caagattgca gaaatataaa
     9421 gcagctgccg attctagctt aaacgatctc gattggacgg accttaaccc atcctccccg
     9481 aactactttc ttttcccgag gactgaagtc ggccgagaac tttatgagtc attttggtct
     9541 gtcggaaaaa tctttgaaga agggtcaacc ggagttttga caggcaggga tcggctttcg
     9601 gttgcagaaa ccgatggcgc gttaagagat cacttagagg cattggaggg ttctgctgtt
     9661 gatcgcgaga tagaaactcg gttcaatctc gaccaagtcg aacactggtc acttagcgat
     9721 acgcgacagc taattcgaga agagggtatc aggcgcaatc taattcggaa attggcatac
     9781 cgtccctttg atacgcagta ttactatgac cacgatgctc tggtgttccg ccgaagacac
     9841 aaagtgatgc aacagatgac tggcggccaa gtaggtcttg ctgtctgtcg gcttacaaag
     9901 ggtggcgatt ggcgccattg tatgattgcc ggcgatccct cggatgactc ttacgtttcg
     9961 gataagagta aggaacgcgc atatctgtac cccctttact ttactcagca gcccgtccgc
    10021 gagtcgaaaa atgcagtagt ccgggaatct tcgatcaacg tcgaaccacc caagaacgaa
    10081 aatctgtcat ctgactttcg cgcatttctc gattctcgct atgatcacca ctatactccc
    10141 gaggaaatac taggctacat ctatgcggtc ttgcacgcgc ccgcataccg cttgctctac
    10201 gccgagttct tgagcatcga tttcccccgc gtgccgtttc ccgagtcaag cgaggagttt
    10261 gaaacccttt ccggcctggg ttgggccttg gtgcaggctc atctcttgcg cgagctgcca
    10321 cgccaaggtt tggccgtcta tcacggcaag ggtgaccacg tggtcgagac cgtgcggtat
    10381 tcgcccgagg agcgagcgat tgcgatcaac aaaacccaat ctttcaaatc agtgccggag
    10441 acagtgtggg agttccgcgt cggcagccat caggtgctcg acaaatactt gaagtcgcgc
    10501 aaaggccgca cactatccct cgacgaaatc aatcatgtcg ccgcaattgt ggatagtttg
    10561 gccttcacga ttgagcagat ggcgaaaatc gacgaggctt acgcatccgc gttccctaac
    10621 cggggataaa tccgggccaa cgctcgcgtc gcgcctcgcc ttggcgcata atgtgctcga
    10681 ttcgtctttt ttgagtggca ttcatggcgc gcgaccacca accggacatg ttttcagaga
    10741 gcacccaatc cgacctgttc ggtgcggagc ctattcctgc gtatcgtccc gatcccgaaa
    10801 aagtgcgcgc gcggctgcac aagattctcg cggaggcgcg ggcggcccgg acattccctt
    10861 gggagccgac gagggtgtcg ctctaccgga ccatctttcc gcaaatgtcg ctctggctgc
    10921 ccgaagatga aagcgcacaa ctgcgctttg agttcgaggc ggaaatcaag cggctggaag
    10981 cggcatgaca ggcgacgtgg actgaacttc aaccgccaat tatgcttcgt cgttccgggt
    11041 gcaggatcga tactgccggt cgtggttatg gatttctgcc tcgctttcct ctgacgtcgc
    11101 tgcctccccc aaccaaacgc ggcctccgct gacggaggcc gcgttagaac acttcatcgc
    11161 acctgggttc gtaacgaagt gtgggagctc ttgaaagcaa ctagaacagc agcagcacgt
    11221 ccatcacctg ttggccgtag cgcggctgtt gcacgtcaga gatctggcag ctgccgccac
    11281 ggcttgccgg ctgcgcatca cctcagcgct gcttgtcttc gcacgcgaac cacccggagc
    11341 gggggcgggg gtcccatatc gcgctgccag atcgttccgt tatcgaactg gatcgtcatg
    11401 ccgtcaggcg aatagacggc gctcacgttc cagtcatcgg cccaaatctt ggtcatcggc
    11461 gcaaacgcat cgggccaggc acgggaaggc tgaccggctt cgttgacgag atttagattc
    11521 catccgttct gcgtgacata ggcaacgctg ccgaccggag ccgcccgaca cccttggatg
    11581 catcgataca tgccggtcag attgaccgtc tgtgccgatg ctgcggttgc agcgaacacg
    11641 gccgcggccg caatggcgca tgatgttatt ctcatgaaat cactccatag ggttacgctg
    11701 atcccaggat ttggggttgc agagggatcc ttaattattc gcatcgcgag cgtttggttc
    11761 catcaactac gacggtaatt cggttccgtt tagcgccacc cctgacggaa cttccccggc
    11821 gatgtcgtcg gatttaggtt ttctactgcg cgggaaggcc agccggccga tcctggaggt
    11881 gaacgccaca aacgaccgcg gcctccgcca ggggagctga cggaggccgc gttggaacac
    11941 ttcatcacgc ccagggttcg cgatgaagtg tgggagctct tgaaaggcta gaacggcagc
    12001 agcacgtcca tcacctgttg gccgtagcgc ggctgttgca cgtcggtgat ctggccgcgg
    12061 ccgccatagg cgatgcgggc ctgcgcgatc ttgctcgaat cgatggtgtt gtcgctctgg
    12121 atgtcctccg ggcgcacgat gccggcgacg atcagttcgc ggatttcgaa attgacccgg
    12181 atctcctgct tgccttcgac cacgagattg ccgttcggca ggagctgcgt caccaccgcg
    12241 gcgacgttgg tctgcagcgc ctcctggcgg ttgaccgaac ccttgccgtc gctggatgac
    12301 gtcgaatccg ccgtcaacag acggccgggc aacacttttg cagcatcccc gccgagcagc
    12361 ttgctgccgg caaaatcggt gaggccggaa tcatccttgg cggtgcggct gcgctgggtc
    12421 tcgttggcga tgttggcctt gtcggtgata ttcaccgtca ccgtcaagat gtcgccgatc
    12481 cgcgccgcgc gctgatcctt gaagaacgcc ctggagccgt tccgccacag cgaattggcg
    12541 ttgtaggacg cggtctccgg tttcggcatc ggcatctgca ccggcttgta gccgggctgc
    12601 gtcgtcggat tttcgatctc ggtcagtttt ggcttttcgc cgatctgcga cagccgatcg
    12661 atcgacgagc atccgctcgc gacactgagg atgaccaaga gcgttccgtt gacaaggagg
    12721 cggttgaacc cagacctgta cattaccatt actctgcttt tggagcggcg tgttgcgcga
    12781 cttgttgcgc ggcttgttgg gtgacttgtt gggtgacttg cgaagtgctg ttgacggcga
    12841 ccgagaccgg ggcggccact tcgctcgacc cgatcgagga ggtggcatcg gcggcagggg
    12901 gaagccgggc cgccggcgcc gataccgtca cctggccgcg gccggatacg acgccggtca
    12961 ccgtgcgctt ggattgcaga ttgagcacgc tgacgacgtc gccttcggtg ccgttgtcca
    13021 gcgccttgcc gcgaatggtc agatagagcc ccgtcgcctc gtaggtcagc gtcacgttct
    13081 ggtcgcgctg caccagatcg ggctttgcga tatcggcgac tcttaaggcc tggccggcgc
    13141 gcagttgttt tcgggcctgc atgccgatcg cgcgatcgcg gctcgcgacg tcgccgccga
    13201 cctcggcctt cggccgccgc tcgaccacca cgtcggagga ttttatcact tcattgcgtt
    13261 cgatgccgcg tgccagcacc gcggcttcca cggtctcgat ggcgctgccg ctgaaacgta
    13321 agcgcgtcgg cgtcgaaaca ttgtcgttgg cgatctcgaa gctgacgtcg aagcggccgc
    13381 tgcgcggatc gaaccgtgcg gaagttgcct gcatggcgcc gctgttggag gcgtcgagcc
    13441 gcagatcctg cacgtcgcgg tcgaaggtca gcgagaggtt ggcggcttcc ccaaggccac
    13501 cccgatgttc cagcgcgcgg gcgacctgca attcgatgtc cttgctctcc agcgtccgcg
    13561 ccagccgggt caccgaaatt tccttcaggt cccttgtatc gaccccgatc acctgatgcg
    13621 cctgcagcgc gctgagcacg cttgcgacct tcagcgagcc ggtggtgccc aaatcgggcg
    13681 cgcgatagat cgcgatctgc gccgaggtgc cggcattgtc gatgacgtcg ccgatccgca
    13741 ccacgtcggc ggtgacgttg acgctggcgc gcagcacggg tgctgcgatg gtgtcgtcgc
    13801 tgacttgcgc ggatgccgcg gttgatgcag ctgcgagcaa agcggtggcg aggaggaggg
    13861 aacgcagggt catcacaaaa ctcccttagc gaaatagcgc ggaggcggat tgcagcatct
    13921 ggtcggcggc gctgatcacc ttggcgttca tctcataggc gcgctgcgcc gcgatcaggt
    13981 cggaaatctc ggtgacgacc tcgacattgg cctgctcgag attgctctgc tgcatgtcgc
    14041 caaagccgtc ggtgttggcg atgccgtcct gcggcgggcc cgatgccggg gtgtcggtga
    14101 acagattgtc gccgatcggc tgcaggccgg ccttgttgat gaaccgcgtc agcccgatct
    14161 ggccgaccgt ggtcggcgtg gtcgatcccg gcaaggtcac cgacaactgg ccctgcgcgt
    14221 tgatggcgat gccggaggca ttttgcggga tggtgatggt cggctgcacc ggattgcctt
    14281 gcgcgttgac gacgcggcct tgcgcgtcca tctggaacga gccgtcgcgg gtataggtga
    14341 agctgccgtc gggcatctgg atcttgaaga aaccttcgcc gcggatggcg atgtcgagat
    14401 cgttgccggt ttgcgccagc gtgccctggc tcatcagccg cggcgtgccg acggttttca
    14461 cgccgccgcc gatgtcgacg ccgaccggca ggatggtgcc ctggtccgac gccgccgcgc
    14521 cgacccggcg cacatggtca tagatcaggt cctggaatgc cgcgcgctgc ttcttgaaac
    14581 cggtggtgcg catgttggcg atgttgttgg agatcacctg aacgttgagt tcctgtgccg
    14641 ccattccggt cgccgcggta taaagagcgc gcatgtcagt gtttccttac gccggaacgt
    14701 cggcgagttt ttcgatcgcg gatttgtgca gatcgctctg ggtctgcagc atggtggcga
    14761 tctgggtgta ggtgcgcgtc acctcgatca tgcggctcat ctcggccacc gagttgacgt
    14821 tggacttttc gacaaaaccc tggcgcacct ttgaggcggt gtcgggctgt ggcgcgccgc
    14881 cttcgccggc cgcatagagg ttggagcctt ccttcagcag cctttgcgcc tgcgcgaaac
    14941 tgaccagccg cagcttgccg cggaccgagt cggtgtggcc gacgccctcg acgacggtga
    15001 cgttgccgtc ggcggcaatg ttgatgtcat ggtcggtcgg ctggaacacg atcgggccgc
    15061 tcgtgccgag caccggatcg ccggcggcgg tcacgagctg gccctgattg ttgatctgca
    15121 agccgccgtc gcgggtgtag cgctcgccgg cgcgcgtctg caccaccaga aagccgccgc
    15181 cgtcgatcgc gacgtcgagc ggattcttgg tctgctcggt cgggccttgg gtaaaatcct
    15241 taaacgtgcc gttgtccagc acatagctga cggcgcggtc acggccgacg aagttgtctt
    15301 cgtgcgcgcc cgagttcaga tattcggaaa acagcgacct gtcggccttg tagccgttgg
    15361 tattgacgtt cgcgacgttg ttggcgacga catccatctg ccgttccagc gtcatctgcc
    15421 gcgaaagtcc gacgagaagc atattctcca tcggtggttc tccccttcaa atgatccgcg
    15481 cggccctccc aggctggttg cggatgatgc gaacctgaag gctctcccaa gccgcggttc
    15541 gctataccca cagcgaaacc cgtgccagtt gagaaaatat cgattcgaca atgggttagg
    15601 tgttttcgcg gacgccgacg gggcggcaat aagggcttgt tgaccatgtt tgcccggcaa
    15661 ctttttccta gctgcgggtc aaacgaaaga atccattaac cattgttacc tagcgtctgc
    15721 atcaagtgag cgcgtgtgcg ccggcggccc tttgtatcgc gtcatcaagc ctgtctcgcg
    15781 catcgaggtt ttttccaacc ggacgggcag gcgatgtcag ataccgattc tgcagatggc
    15841 ggcgcggcgg agccagttgg tgctcccaag ggcaagctca agctgatcat cgccgcggta
    15901 ggcctgttcg tcattctcgg cggcggcgct gcgacctggt tcatgttctt tcgcggccac
    15961 agcgaagaag tgcacgccga ggctgcggcg gcgccgaagc cggcgattta catcgacgtg
    16021 ccggaaatgc tggtcaatct ggtcggcgcg cccggcgatc gcgtgcaata tctcaaggcc
    16081 aggatcgtgc tcgaggtcaa agaggaaaag caggtcgagg cgatcaagcc gacgatgccg
    16141 cgcgtcaccg atatcttcca gacctatctg cgcgagctgc ggccggccga tctcaccggc
    16201 tcggcgggcc tgtttcgtct caaggaagag ctgaccaagc gggtcaatac ggcgatctcg
    16261 ccaagtcagg tcaacgccgt gctgttcaag gaaatcgtcg ttcagtgacg agacggattt
    16321 aaaaaccatg gccggcaaca gcatcgacga ccaggacgcc atcgcagccc aatgggaagc
    16381 gtcgctggat tccgaggatc cggcggaggc cgcggccgcg gccgcggcca atgaactctc
    16441 cggcaccatg gcgctgcaat gggccgccat ggtcgaagac ggcagccgcg acttcggcgg
    16501 cggcaagaac ggcggcgagc gggtgctgtc gcaggaggaa atcgacaacc tgctcggctt
    16561 caatgtcggc gaggtcaacc tcgacgacaa ttccggcatt cgcgccatca tcgattcggc
    16621 gatggtgtcc tacgagcgtc tgccgatgct cgaaatcgtg ttcgaccggc tggtgcggct
    16681 gatgaccacg tccttgcgca acttcac
//
LOCUS       DASWGM010000323        11191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2656564,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000323 DASWGM010000000
VERSION     DASWGM010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2656564"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VGA15_09255"
     CDS             complement(<1..530)
                     /locus_tag="VGA15_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEX9587920.1"
                     /translation="MRTVFALPAAAISFLVSSAPAYADLKLCNRMSYVVEAAIGIDDK
                     SATATRGWFRIDPAACRVVAQGTLTADRILLTARALGVYGSSPIPQSGGDTLCIARDN
                     FVIAAARQCRGGQSPAPFTQINPTRADDGNQVAYLAEDSEYDDEQARLAGIQRLLVIA
                     GYDAAPIDGVDGPKTQA"
     gene            complement(596..1492)
                     /gene="argB"
                     /locus_tag="VGA15_09260"
     CDS             complement(596..1492)
                     /gene="argB"
                     /locus_tag="VGA15_09260"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX9587921.1"
                     /translation="MTDAPHISPLDQARILSEALPHMQRYDEETIVIKYGGHAMGAED
                     LAKAFARDIVLLEQTAINPVVVHGGGPQIAQMLQRLGIKSEFAAGLRITDAATIEIVE
                     MVLAGSINKALVGYINEAGGKAVGLCGKDGNMVTASKVTRTMVDPDSNIEKVVDLGFV
                     GEPEKVDLTLLNQLIGHDLIPVLAPLAISRTGQTFNVNADTFAGAVAGALKAKRLLLL
                     TDVPGVLDKSNKLIPELSVKDARKLIADGTISGGMIPKVETCIYALEQGVEGVVIIDG
                     KTQHAVLLELFTNQGTGTLIHK"
     gene            complement(1732..2109)
                     /locus_tag="VGA15_09265"
     CDS             complement(1732..2109)
                     /locus_tag="VGA15_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX9587922.1"
                     /translation="MMPSRLFRILIVLAGIMGADGVVLAAASAHQADASRLASASSML
                     LFHATAVLAVVALAERGVIHLRIGIAAAFGFVIAAALFAGDLTLRQYAGHSLFSMAAP
                     TGGTLLIVSWLVLALAAAWPRRG"
     gene            complement(2106..2759)
                     /gene="yihA"
                     /locus_tag="VGA15_09270"
     CDS             complement(2106..2759)
                     /gene="yihA"
                     /locus_tag="VGA15_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX9587923.1"
                     /translation="MTADIDADLIEQGRKLFAGDWQFFWASPSIETLPPMAGMEVAFA
                     GRSNVGKSSLINALTGRNALARTSHTPGRTQELIFFEGPDKAGFRLVDMPGYGYASAP
                     KAKVASWTALIHKFLQGRGSLARVYVLIDARHGFKEVDLDVLKTLDKSAVSYQVVLTK
                     ADQVKPAELEQRAAEVQAALAKHPAAFPELLVTSSRTGAGMPELRAAMVRLLGERSR"
     gene            complement(2957..4831)
                     /gene="yidC"
                     /locus_tag="VGA15_09275"
     CDS             complement(2957..4831)
                     /gene="yidC"
                     /locus_tag="VGA15_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX9587924.1"
                     /translation="MTDNRNTILAVILSGLVLIAWQYFYNMPQMEKQRAAQQAQSELV
                     KPTPQAGSTATSTTPQTGAAPSPSANAPTVNQPASAAPVVSRDTAIAAGPRIKIDTPR
                     ISGSISLKGARIDDLALVQFRETVDPSSPAIVLFSPSGTANPYYAEFGWVPASGSTVK
                     LPDQNTLWQQEGANSLSPNLPVTLKYDNGDGLTFHRTIAIDDRYLFTIKDEVTNVGNA
                     PVTLYPFALISRHNTPQVAGYYILHEGLVGYLGDQGLQEYGYKKIDDAKTVSFKVTNG
                     WLGITDKYWASALLPDTNAQLQARYSSNLVGTVRTYQTDYLQDQQTIAIGGTGSANAR
                     LFAGAKEASVVGINFPIGGLGGYNKELGLNHFDLLIDWGWFYFITKPMFLALDFFYHL
                     VGNFGISILLVTVLVKLLFFPLANKSYASMAKMKSVQPQLAALKERYPDDKVKQQQEM
                     MEIYKKEKINPIAGCLPVALQIPVFFSLYKVLFVTIEMRHAPFFGWVKDLSAPDPTNL
                     FNLFGLLHFDPTTVPLFGHYLALGVWPIIMGITMWFQMKLNPTPPDPTQKMIFDWMPL
                     IFTFMLAGFPAGLVIYWAWNNTLSVLQQSFIMRRNGVKVELFDNLRATFASKKAESKT
                     "
     gene            complement(4950..5303)
                     /gene="rnpA"
                     /locus_tag="VGA15_09280"
     CDS             complement(4950..5303)
                     /gene="rnpA"
                     /locus_tag="VGA15_09280"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX9587925.1"
                     /translation="MDRLRQRADFLAVAGGARADCAAFVLQSRRRDDDGPIRVGFTVT
                     KKNGTATERNRIRRRLRELVKRLDVISMRPHSDYVLVGRRAALNRDFATMLDDLRSAL
                     DRLDRQPKRPRGETS"
     gene            complement(5328..5462)
                     /gene="rpmH"
                     /locus_tag="VGA15_09285"
     CDS             complement(5328..5462)
                     /gene="rpmH"
                     /locus_tag="VGA15_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX9587926.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARLATTGGRKVLAARRARGRKRLSA
                     "
     gene            6005..7483
                     /locus_tag="VGA15_09290"
     CDS             6005..7483
                     /locus_tag="VGA15_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9587927.1"
                     /translation="MSVTEEQLITGSPRPLPSRRLGLSGKLLLLTIPLVMIVGFLIYV
                     PAIANFRMNRLNDRLAAANTAALVLDAAPSGMVPDSLARQILTSIGARAVAIKMGQQR
                     RLLASAGLPPAIDHDVDMRSLTAWSAIVDSFETMLETGNEAIRVIGETPGGGQFIEVV
                     IDELPLRQAMYRFSSNLLLVSLGLATLTAGLVYFALHYLFVRPMRRLTANLVGFHENP
                     ESSARIIAPTQRGDEIGVAERELSDMQRDLVSMLHQKSRLAALGLAVSKINHDLRNLL
                     ASSQLLSDQLASVPDPRVQRFAPKLMRSLERAIAFCQSTLSYGRAQEAAPDRRMILIE
                     PVVVEVRESAGLASDASIVWISAIERGLAIDADPDQLFRVLLNLVRNAAQALENRPRS
                     DAAIQQIRITGRREGSVAIVEVSDTGPGVPAKARDHLFEAFQTSGRPGGSGLGLAIAA
                     ELVRAHDGDIHLVEGTIGATFRIAIPDRPVELLSVRNERALA"
     gene            7849..7925
                     /locus_tag="VGA15_09295"
     tRNA            7849..7925
                     /locus_tag="VGA15_09295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7883..7885,aa:Arg,seq:acg)
     gene            complement(7896..9530)
                     /locus_tag="VGA15_09300"
     CDS             complement(7896..9530)
                     /locus_tag="VGA15_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9587928.1"
                     /translation="MAAAVRAALYLRVSTGRQADNDLSIPDQRRQAKAYCASRGWEIA
                     ADYVEPGASATDDRRPEFQRMIDAATTKPAAFDVIVVHSFSRFFRDQFQLEFYVRRLA
                     KAGVRLVSITQELGDDPMSNMIRQIMALFDEYQSKENAKHTLRAMKENARQGFWNGAL
                     PPIGYRIVEAAEQRGHRTKKSLEIDPIQAETVRSIFRFAREGNGSSGPMGVKSIAKHL
                     NEARIRTRDGGRWGVDAVHKVLTRTTYIGRHRFNTKYWKTRERKPEAEVVEMIVPPII
                     DVAEFEAVQTLLKARSPAMTAPRVVSGPTLLTGICFCAACGGAMTLRTGKSGRYRYYT
                     CCTKARQGETGCKGRTIPMDKLDSVVAEHIEHRLLQPKRLEQILSRVLDRREELAERR
                     TAHIAELRKRASEAEAKLKRLYYAIEDGIADVSDPMLKERVTELKAVRDQARADAERA
                     EGALDRLGPCITPQALKTFASQARKRMRIEGGGYRRDHLRALAQRIEVDAKEVRIMGS
                     KSELLRTLVAASSAKTAGFGVPSFVPKWRARRDSNS"
     gene            complement(9544..9897)
                     /locus_tag="VGA15_09305"
     CDS             complement(9544..9897)
                     /locus_tag="VGA15_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587929.1"
                     /translation="MLCAIAPKLQRKREVAALQRDMAWSSAVLWIGQACHTGAMAPAQ
                     ESRRRRVPADASLDDLYDYVAAPARPGRRRANRASVTLTVTDNWPEDVPVTEAEIAVF
                     EAWFGELFDELFGEG"
     gene            complement(10360..>11191)
                     /locus_tag="VGA15_09310"
     CDS             complement(10360..>11191)
                     /locus_tag="VGA15_09310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9587930.1"
                     /translation="ATLVGAALHDGAIGSLDDRCEQYLPRLRGSAYEGVTIRNLLRMC
                     SGVAWREENDADGRSEVFRLSKAMVSRRPGSVCKLPSAQPQGVVFNYSTGESCLIGAL
                     VVAATGRPLADYCAEKIWRPAGMEADGYWQLESDDGLELGGLGVSARLRDFGRFGQLM
                     LEDGEAFSGRRVLPPGWRDLAGQPDSAPTGFGRLMPGSPAGYGYHWWAVPPLPGGVNN
                     GAFSARGTFGQFIFVNPTEQVVIAIQSAWRQPQDSDAEVEIVAMIRAAVSALRTEPAS
                     "
BASE COUNT         1974 a         3516 c         3599 g         2102 t
ORIGIN      
        1 gcctgggtct tcggaccatc gacgccatcg atcggcgcgg catcataacc tgcgatcacc
       61 agcagccgct gaatgccggc gagccttgcc tgctcgtcat catattcgct gtcctcggca
      121 agataggcca cctgattgcc gtcgtcggcc cgcgtcgggt tgatctgggt gaagggcgcc
      181 gggctttgcc cgccgcggca ctgacgcgcg gcggcgatca cgaaattgtc gcgcgcgatg
      241 caaagcgtat ccccgccgct ctgcggtatc ggcgacgagc cgtagacgcc gagcgcgcgg
      301 gcggtaagca ggatgcggtc ggcggtcagc gtgccctgcg cgacgacgcg gcacgccgcc
      361 ggatcgatgc gaaaccagcc gcgcgtcgcg gtggcggact tgtcgtcgat gccgatcgcg
      421 gcctcgacca catagctcat gcgattgcag agtttcaggt cggcgtaggc cggcgctgag
      481 gagacgagaa acgagatcgc cgcggcggga agcgcgaaga ccgtgcgcac cccgtatcgc
      541 cgcatcgctg cgctgaagcg gggcgtcaac ctggggagcg gtgaaatcgc gcgagtcact
      601 tgtggatcag cgtgccagtg ccttggttgg tgaacaattc gagcaacacc gcgtgctggg
      661 tcttgccatc gatgatgacg acgccctcga cgccctgctc gagcgcgtag atgcaggttt
      721 cgaccttggg gatcatgccg ccggaaatcg tgccgtcggc gatcagcttg cgggcgtcct
      781 ttaccgacaa ttccggaatc agcttgttcg acttgtcgag cacgccgggg acgtcggtca
      841 aaagcaagag ccgcttggcc ttcagcgcgc cggcgaccgc gccggcgaag gtgtcggcgt
      901 tgacgttgaa agtctggccc gtcctcgaaa tcgccaatgg cgccagcacc gggatcagat
      961 catggccgat cagctggttc agcagcgtca ggtcgacctt ttccggctcg ccgacgaaac
     1021 cgagatcgac caccttttcg atgttcgaat ccggatcgac catggtgcgc gtcaccttcg
     1081 acgccgtcac catgttgccg tccttgccgc acagcccgac cgccttgccg ccggcctcgt
     1141 tgatgtagcc gaccagcgcc ttgttgatcg aacccgccag caccatctcg acgatctcga
     1201 tggtcgcggc atcggtgatg cgcaggccgg cggcgaattc cgatttgatc ccgagccgct
     1261 gcagcatctg cgcgatctgc ggtccgccgc catgcaccac caccggattg atggcggtct
     1321 gctccaatag aacgatgtcg cgcgcgaacg ccttggcgag gtcttcggcg cccatcgcat
     1381 ggccgccata tttgatgacg atggtttcct cgtcatatcg ctgcatgtgc ggcagcgcct
     1441 cggacaggat gcgggcctga tcgagcggac tgatatgggg cgcgtcggtc atgaagcggg
     1501 tctcgtgggt cagcggggtg gggcgggttc tatctgattg gcggccacgg cgcaaagtgc
     1561 cgttgctttc ttcccctctc cccttgtggg agagggtggc tcgaatgagc ggagcgaatt
     1621 cgagacgggt gaggggttcc ggtctatcaa taagcgccgt aacccctcat ccggcacgct
     1681 tcgcgcgcca ccttctccca caaggggaga agggagaaag agtgttccgc ctcaaccccg
     1741 ccgcggccat gccgcggcaa gcgcgagcac cagccagctg acgatcagaa gcgtgccgcc
     1801 ggtcggcgcg gccatcgaaa acaggctgtg gccggcatat tgccgcagcg tcagatcgcc
     1861 ggcgaacagg gccgccgcga tgacaaagcc gaatgcggcg gcgatgccta ttcggagatg
     1921 gatgacgccg cgctcggcca acgccaccac ggcgagcacg gcggtagcat gaaacagcag
     1981 catcgatgag gccgacgcca gccgcgaggc atcggcctga tgcgcggagg ccgcggccag
     2041 gacgacgccg tcggcgccca tgattccggc caggacgatc agaatgcgaa ataggcggga
     2101 tggcatcatc ggctgcgctc gccgagcagg cgcaccatgg cggcgcgcaa ttccggcatt
     2161 ccggcgccgg tgcgcgacga tgtcaccaat agctctggga aggcggcagg atgcttggca
     2221 agcgctgcct gaacctcggc ggcgcgctgc tccagttcgg ctggcttcac ctgatcggcc
     2281 ttggtgagga ccacctgata gctgacggcc gatttgtcga gcgtcttcag gacgtcgaga
     2341 tcgacctcct taaaaccatg ccgggcgtcg atcagcacat agacccgcgc cagactgccg
     2401 cggccctgca ggaatttgtg gatcagcgcg gtccatgacg cgaccttggc tttcggcgcg
     2461 gaggcgtaac cgtagccggg catatcgacc agccgaaagc cggccttgtc agggccttcg
     2521 aagaagatca attcctgggt gcggcccggg gtgtgggagg tgcgcgccag cgcgttgcgc
     2581 ccggtcagcg cgttgatcag gctcgatttg ccgacattgg agcggccggc gaacgcgacc
     2641 tccatgccgg ccatcggtgg cagcgtttcg atcgagggcg aggcccagaa gaactgccag
     2701 tcgccggcga acaacttgcg accctgctcg atcaggtcag catcgatatc cgcggtcatg
     2761 cgaaggcttt ctgttcctcg tcatggccgg gcatagcatg tctgcgttgc gcagactgcg
     2821 taaacttgtc tgcgatgccg gccatctacg tctttccttc tcgctaacgt cgtaaagacg
     2881 tggatgccca gcacaaggcc gggcatgacg agttggggtt ctcgtgtcct gcaccaagca
     2941 gcagcaagcg tggccgtcag gttttcgact cggccttctt cgaggcaaac gtcgccctga
     3001 gattgtcgaa caattcaacc ttcacgccat tgcggcgcat gatgaagctc tgctgcagca
     3061 ccgacaacgt gttgttccag gcccagtaga tcacgagacc ggcggggaag cccgccagca
     3121 tgaaggtgaa gatcagcggc atccagtcga agatcatctt ctgggtcgga tccggcggcg
     3181 tcggattgag cttcatctgg aaccacatcg tgatacccat gatgatcggc cagacgccga
     3241 gcgcgaggta gtgtccgaac agcggcacgg tggtcggatc gaaatgcaac aggccgaaca
     3301 ggttgaacag gttggtcgga tcgggcgccg acagatcctt gacccagccg aagaacggcg
     3361 catgccgcat ctcgatggtg acgaacagca ccttgtagag tgagaagaac accggaatct
     3421 gcaaggcgac gggaagacag ccggcgatcg ggttgatctt ctccttcttg tagatttcca
     3481 tcatctcctg ctgctgctta accttgtcgt cgggatagcg ttccttcagc gcggccagtt
     3541 gcggctgcac cgatttcatc ttggccatcg acgcgtagga cttgttggcg agcgggaaga
     3601 acaacagctt caccagcacc gtcaccagca ggatagagat gccgaaattg ccgaccaggt
     3661 gatagaaaaa gtccagcgcc aggaacatcg gcttggtgat gaagtagaac cagccccagt
     3721 cgatcagaag atcgaagtgg ttgagcccga gctccttgtt gtagccgccg agacccccga
     3781 tcgggaaatt gatgccgacc acgctggctt ccttggcgcc ggcgaacagc cgcgcattgg
     3841 cgctgccggt gccgccgatc gcgatggtct gctgatcctg caggtaatcg gtctgatagg
     3901 ttcgcacggt gccgacgaga ttggacgagt agcgcgcctg cagctgcgcg ttggtgtccg
     3961 gcagcagcgc cgacgcccag tatttgtcgg tgatgccgag ccagccattg gtgaccttga
     4021 agctgaccgt cttggcgtcg tcgattttct tgtagccgta ttcctgcagg ccctgatcgc
     4081 cgagataacc gaccaggcct tcgtgcagga tgtagtagcc ggcgacctgc ggggtgttgt
     4141 gccgcgaaat cagcgcgaac ggataaagcg tgaccggtgc attgccgacg ttggtgactt
     4201 catccttgat ggtgaagaga tagcgatcgt cgatcgcaat ggtgcggtgg aaggtgaggc
     4261 cgtcgccatt gtcgtatttc agggtcaccg ggaggttcgg cgagaggctg ttcgcgcctt
     4321 cctgctgcca cagcgtgttc tggtcgggaa gcttcaccgt cgaaccgctg gcaggcaccc
     4381 agccgaattc ggcataataa ggattggccg tgccagaggg cgagaacagc acgatcgcgg
     4441 gcgaagacgg gtcgaccgtt tcgcggaatt gtaccagcgc caggtcgtcg atgcgcgcgc
     4501 ccttcagcga aatgctgccg ctgatgcgcg gggtgtcgat cttgatgcgg gggcctgcgg
     4561 caatcgcggt atcgcggctg acgacaggcg ccgccgaagc aggctgatta accgtaggcg
     4621 cattcgcgga gggcgagggc gccgcgccgg tctgtggggt tgtggaagtg gccgtgctgc
     4681 cggcttgcgg ggtgggcttc accaattcgc tctgcgcctg ttgcgcggcg cgctgctttt
     4741 ccatctgcgg catgttgtag aaatactgcc aggcgatcag cacaaggccg gacagaatga
     4801 cggcgaggat ggtgttgcga ttatcggtca tcgttcaagg tctcgtcatt cagttcggtt
     4861 tgcggcttgc gctagagtct ttacgtcttg acgcgttttc tttacgcgaa ccgtgtccac
     4921 ttcgctcgaa tacgctttag acggtctcgt cacgaggtct caccacgcgg tcttttgggc
     4981 tgccggtcga ggcgatcgag cgccgagcgg agatcgtcga gcatggtcgc aaaatcacgg
     5041 ttcagtgcgg cccgacggcc gactagcaca taatcactgt gcggtcgcat tgatatgacg
     5101 tccagccgct tcaccagttc gcgaagcctg cgccggatgc gattgcgctc ggtggcggtg
     5161 ccgttctttt tggtaacggt gaagcccacc cggatcgggc cgtcatcgtc gcggcggcgg
     5221 ctttgcagca caaaggccgc acaatcggcc cgcgcgccgc cggcaacggc gaggaagtcc
     5281 gcccgctgcc tcagccgatc catggtggag tctccgggaa gcccggttca ggcgctcaaa
     5341 cgcttgcggc cccgggcgcg gcgcgcggcg aggaccttgc ggccgccggt ggtcgcgaga
     5401 cgggcgcgga agccgtgacg gcgcttgcgc accagtttgc tgggttgata agtccgcttc
     5461 acgggtcgtt ctccgctgac cgggcaattt gcctgattaa tgtgatgagt ccggtgatgt
     5521 tggccggccc aaagcgggcc atttcgcagc ccaaacgagc tgccccggtc ggaccgggtc
     5581 atcgcggaca gttggcgcgg cttatacggg agcgacccgt tttcgtcaat ctggccggag
     5641 gtgtggtcgt tcggaagttc tcttcatatc cgccgcgcgg ccttgaggag agatttcgaa
     5701 ccaaaaccgc actggaatca tgggtttcca gtgtcctttc gcgtccggcg ctcgcgacgg
     5761 catcgctgcg atggtgcgaa agctcgggtc cgggacacta ggccacatcc gaatttccgc
     5821 aaaaccgtgc aaatcgtccg aatatgtcaa cggtaacggt gtttttcagt gtgagcatgg
     5881 cggctcgccg gtccgcgcat tagatcggga agaggcagac attagcgatc cgtaatttga
     5941 ccggtcccgg ggcaaaacgc atcctgaacc gcgcggctaa gaaaggcata taaaggcata
     6001 tatcgtgtca gtgaccgagg agcagctgat aaccggatcg ccgcggcccc tgccgtcgcg
     6061 ccggcttggt ctgtccggca agctgctgct gctcaccatt ccgctggtca tgatcgtcgg
     6121 attcctgatc tatgtgccgg cgattgcgaa tttccggatg aaccggctga acgaccgtct
     6181 ggcggcggcg aacaccgccg cactggtgct ggatgccgcg ccctccggca tggttccgga
     6241 ttcgctggcc cggcaaatcc ttaccagcat tggcgcgcgc gcggtcgcca tcaagatggg
     6301 gcagcaacgc cggctattgg cgagcgccgg tttgccgccg gcgatcgatc acgatgtcga
     6361 catgcggtcc ctgaccgcgt ggtccgcgat cgtcgattcg ttcgagacca tgcttgaaac
     6421 cggcaacgag gcgatccgcg tgatcggtga gacgcccggc ggcggacagt tcatcgaagt
     6481 cgtgatcgac gaactgccgc tgcgtcaggc gatgtaccgg ttttcgagca acctgctctt
     6541 ggtttcgctg gggctcgcga ccctcaccgc gggactggtt tatttcgccc tgcattatct
     6601 gttcgtgcgg ccgatgcgac ggctgaccgc caatcttgtc ggctttcacg agaatcccga
     6661 gagttcggcg cggatcatcg cgcccactca gcgcggcgac gagatcggcg tcgccgagcg
     6721 cgaattgtcc gatatgcagc gcgacctggt gtcgatgctg catcagaaga gccggctcgc
     6781 ggcccttggc cttgcggtat cgaagatcaa ccatgatttg cgcaacctcc tggcttcctc
     6841 gcaactgctg tcggaccagc tcgccagcgt gccggatccc agggtgcagc gctttgcgcc
     6901 gaaactgatg cgctcgctgg agcgcgccat cgcgttctgc cagtcgacgc tgtcctatgg
     6961 ccgggcgcag gaggccgccc ccgatcgccg catgatcctg atcgagcctg tggtcgtcga
     7021 agtccgcgaa tccgcggggc tggcctcgga tgcgtcgatc gtctggatct ccgcgatcga
     7081 gcgcgggctt gccatcgacg ccgaccccga tcagctgttt cgcgtgctgc tcaacctggt
     7141 gcgcaacgcg gcgcaggcgc tcgaaaaccg cccgaggagc gacgccgcca tccagcagat
     7201 ccgaatcacc ggccgccgcg aaggctcggt cgccatcgtc gaggtttccg acaccggccc
     7261 tggcgtgccg gccaaggccc gcgaccatct gttcgaggcg ttccagacct cgggcaggcc
     7321 cggcggcagc gggctcggcc ttgcgatcgc cgccgaactg gtccgcgccc atgacggcga
     7381 catccatctg gtggaaggaa ccatcggcgc gaccttccgg atcgccatac cggaccggcc
     7441 ggtggaacta ttgagcgtgc gcaacgagcg ggcgctggca taaaacaccg cgcgccttct
     7501 gttttcgtca ttccggccct gtttccgtca ttccggggcg aggccaacgg gtccgcgcaa
     7561 agcgcggccc gatgacaggc tccgcctcga gcccggaatc catactcccg atcgtggtca
     7621 tggattccgg gcccgcgcca agcggcgcgt cccggaatga cgaagagagc cttttgagga
     7681 acttgctcgt gttttcccac gcaaatcgac cggcccacgc cccttaccgg accttgccaa
     7741 gcccgaccgg agccggtagc tatggcgcgc tttcgcgacc tctcacatgt ttgtcgaatg
     7801 caggcggcat cggcgggcct taacgcccct cgaacgccag cgaaaaacgc gcccgtagct
     7861 cagctggata gagcactaga ctacgaatct aggggtcagg agttcgaatc tcttcgggcg
     7921 cgccacttcg gtacaaaact gggcactcca aaacccgccg tttttgcgct tgaagcggcg
     7981 acgagcgtgc gcagtagttc gcttttcgag cccatgatgc gaacttcttt cgcatcgact
     8041 tcgatgcgct gagccagcgc gcgcaggtgg tcgcggcggt agccgccgcc ctcgatccgc
     8101 atgcgcttgc gggcctggct ggcgaaggtt ttgagcgcct gcggcgtgat gcacggtcca
     8161 aggcggtcaa gcgcgccctc ggcccgctct gcgtcggcgc gggcctgatc gcggacggcc
     8221 ttcaactcgg tcacacgctc cttgagcatc ggatcggaca cgtcagcgat tccgtcttcg
     8281 atggcatagt acagccgctt gagcttggct tccgcttcgg atgcacgctt gcgcaattcg
     8341 gcgatgtgcg ccgttcggcg ctcggcgagc tcctcacgac ggtcaagcac acgcgacagg
     8401 atttgctcga gacgtttggg ctgcaaaagg cgatgctcga tgtgctcggc taccacacta
     8461 tcgagcttgt ccatcggaat ggtgcggccc ttgcagccgg tctcgccttg ccgggccttg
     8521 gtgcagcaag tgtaatacct gtatctgccg ctcttcccgg ttctcagtgt cattgccccg
     8581 ccgcaggcgg cgcaaaagca gatgccagta aggagcgtcg gtccgctgac gacgcgcggc
     8641 gcggtcattg cggggctgcg cgctttgagc aacgtctgca cggcctcgaa ctcggccacg
     8701 tcgatgatcg gcggcacgat catctcgacc acctcggcct cgggtttgcg ctcgcgcgtt
     8761 ttccaatact tggtgttgaa gcggtggcgg ccgatatacg tcgttcgcgt gagcaccttg
     8821 tgcacggcat cgacgcccca gcgcccgccg tcgcgcgtgc ggatgcgggc ttcgttcaga
     8881 tgtttggcga tcgatttgac gcccatcggt cccgatgagc cgttgccttc gcgtgcgaag
     8941 cggaagatcg accgcacggt ctcggcctgg atgggatcga tctcaagcga cttcttggta
     9001 cggtggccac gctgctcggc ggcctctacg atgcggtagc cgatcggcgg cagcgcgcca
     9061 ttccagaagc cttgtcgggc gttctccttc atcgcccgca gcgtgtgctt ggcgttttcc
     9121 ttggactggt attcgtcgaa cagcgccatg atctgcctga tcatgttgct catcgggtcg
     9181 tcgcccagct cctgcgtgat cgacaccagc cgcacgccgg ccttggcaag ccggcggacg
     9241 taaaactcaa gctggaactg gtcgcggaag aagcggctga agctgtgcac cacgatcaca
     9301 tcgaacgccg ccggcttggt ggtcgcggcg tcgatcatgc gctggaactc cggccgccga
     9361 tcgtcggtcg cggacgcgcc gggctcgaca taatccgcgg cgatctccca gccgcgcgac
     9421 gcacagtagg ctttcgcctg gcggcgctga tccgggatgg agagatcgtt gtcggcttgc
     9481 cgccccgtcg agacccgcag gtaaagagcc gcccgaactg ccgctgccat ggtgatgtcc
     9541 ccctcagccc tctccaaata gctcgtcgaa caattcgcca aaccacgcct cgaacacggc
     9601 gatctcggcc tcggtcaccg gcacgtcttc cggccagttg tccgtcaccg tcaatgtcac
     9661 cgaagcgcgg ttcgcccgtc gacggccagg acgagcgggc gcggccacat agtcatagag
     9721 atcgtcgagt gacgcgtcgg ccggcacgcg ccgtcgccgc gattcttgag cgggagccat
     9781 ggcgccagtg tggcaagcct ggccgatcca cagaacggcc gaactccacg ccatatcacg
     9841 ctgcagtgct gcgacctcgc gcttcctctg cagcttgggc gcaatcgcgc acagcaagcc
     9901 atgatcgcat ggcggcaaag agccgggcca gcggagcgca gtcaaggccg caagccggcg
     9961 gagccggtcg cgcgaagcgc gagccttgac ggtgcaccgt tggtccggca gcggcctccc
    10021 atgcgatcat ggccggacat catgccgagc acgcgcggca aaccgctacg ctgcgatggg
    10081 tggcgctcgg gatattgatt ccgaacacct ttttcgttga agcggcactg gggcgttttg
    10141 cgcggtgccg ttacagtgcc atccaaaaca atgtgcagac aaacacttac gaccctcgac
    10201 ggcacggagc gacaccgtcg cttttatctt gccgtcctaa tccgtcggtc tgcacagaaa
    10261 aaattagccc acatgaagtt gcttgacccg cacatctgga gtgtaggaag cgatctgtcg
    10321 gtatcgcgag cggtcagacg ggagcatcgg gctgagggcc tacgacgctg gctccgttcg
    10381 cagcgcgctt accgcagctc tgatcatcgc gacgatttcg acctcggcat cgctgtcctg
    10441 aggttgacgc cacgcgctct ggattgcaat gaccacctgc tcggtcggat tgacgaagat
    10501 gaactggcca aaggtgcctc tcgccgagaa ggcaccgttg ttgacgccgc cgggcaaggg
    10561 cggcaccgcc caccagtgat agccgtatcc cgccggtgaa ccgggcatga gccgaccgaa
    10621 gccagtgggc gcgctgtcgg gctgtccggc gagatcgcgc cagccgggcg gtagcacccg
    10681 tcggccactg aacgcttcgc catcctccaa catcagctgg ccgaagcggc cgaagtcgcg
    10741 caggcgcgca ctgacgccaa gcccgcccag ctccaggccg tcatcggatt cgagttgcca
    10801 atacccgtcg gcctccatgc cggcaggtct ccaaatcttc tcagcgcagt agtcggccaa
    10861 gggccggccc gtagcggcca cgaccagcgc gccgatcagg cagctctcgc cggtcgaata
    10921 gttgaagacg acgccctgcg gctgagcgct cggcagtttg cacacggagc ctggccgtcg
    10981 gctcaccatg gccttgctca gtcgaaagac ctctgagcgt ccgtctgcat cgttttcctc
    11041 gcgccaggct acaccggagc acatccgcag gagattacga atcgtgacgc cctcgtaagc
    11101 cgagccgcgc agccgcggca ggtattgctc gcagcgatcg tcaaggctgc cgatcgcgcc
    11161 atcgtggagc gcagccccga cgagcgtcgc g
//
LOCUS       DASWGM010000324         7421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_862800,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000324 DASWGM010000000
VERSION     DASWGM010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862800"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VGA15_09315"
     CDS             <1..343
                     /locus_tag="VGA15_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269553.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEX9587931.1"
                     /translation="QILVNNEPVDALSMLVHRQRAEGRGRAMVEKMKELIPPHMFQIP
                     IQAAIGGKVIARETVRALRKDVTAKCYGGDITRKRKLLEKQKEGKKKMRQFGKVDIPQ
                     EAFIAALKVDS"
     gene            583..702
                     /locus_tag="VGA15_09320"
                     /pseudo
     CDS             583..702
                     /locus_tag="VGA15_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(720..1148)
                     /locus_tag="VGA15_09325"
     CDS             complement(720..1148)
                     /locus_tag="VGA15_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587932.1"
                     /translation="MTEELFKFWLLVWGAGLSTVLGLIKLWETLWRDRIKLSTTYSFT
                     GQKGVDDEITIVNLSDRTVQVAHWSLAWQGAFSAAVTDLTPDNGGYRFTIDPKSEHTI
                     AFSDENKFNWGSKAANGRKMYLTLLLFGRRRPMKLLVKAD"
     gene            complement(1270..2184)
                     /locus_tag="VGA15_09330"
     CDS             complement(1270..2184)
                     /locus_tag="VGA15_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9587933.1"
                     /translation="MAKIDPSVLKRLREAKGWTQEALSENTKFGRDPKIDKRTISRLE
                     RGKQANTHGRTIRQLARALAVEPPVLMGEVPIPETRLQDQSASQSNIQISSHASNALY
                     LIYERYHVRPWQVIEFAPFLLCWVAEASLRQRRSRLEELERSYQVTRDRERQIEHTRK
                     SDFDDLEEKIRAERESIESRDLFGYQLDEAGFVQYRHEWTENPFARFLSGLADDFEGV
                     IDFEGFSTIDYPEYTVCPDEAERLVGGDQSLAKRILDGTVALNEMPEEIRDSYNSAVR
                     AQWVSGKVAEFLERMERQRRPVRKEAST"
     gene            2427..2615
                     /locus_tag="VGA15_09335"
     CDS             2427..2615
                     /locus_tag="VGA15_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HEX9587934.1"
                     /translation="MASRRERLRAQGLRPVQHWVPDLRDPKVLAEIRREIKLMAQHPE
                     NDAIDDWIESVYDWESWK"
     gene            2636..2941
                     /locus_tag="VGA15_09340"
     CDS             2636..2941
                     /locus_tag="VGA15_09340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX9587935.1"
                     /translation="MVVPSDPGRPRPGIVVQGDKFNAGLSTIFVCPVSSDLQDGLSLR
                     PLIEVAPENGLRLRSQIMTDKLVALRRDRIRRVIGRIDAETSEHLDRTLLVVLGLAP"
     gene            3059..4234
                     /locus_tag="VGA15_09345"
     CDS             3059..4234
                     /locus_tag="VGA15_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9587936.1"
                     /translation="MNKLPGFGLIERARALAPLITREADEIERTRRLTEPVVSALIEN
                     GLYRVLLPQSLGGTEAPPEIFMQMLEEVAKADASTAWCLGQCSVCAMTAAYLDPDAAN
                     QIFNAPPGILAWGAIAHEAQAVPGGYRANARWDFASGSRQASWLGAHVQIIEADGTRR
                     LKPNGAPEVRTILFPLASATMYDVWDVIGLNGTGTDSYSVDNLFIPEKFTALRDDPTA
                     LREKGPLYRLTTNMVFGLGFAAVSVGVARATLDAAVDLARSKTPAGLKKMHENNAVQG
                     AIGRNEASLRAARAYLYATAAEVWRDLTRNGNFTEEHRAALRLASTWTIHQAASVVDA
                     AYHMAGATAVFSANRFERRFRDMHAIAQQIQARDTHYEDVGKMILTADPGTSANNAQ"
     gene            4445..6412
                     /locus_tag="VGA15_09350"
     CDS             4445..6412
                     /locus_tag="VGA15_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9587937.1"
                     /translation="MFKLRSISARLILTISVTVAVVCGILGTFSVVQQRSLTRLALDQ
                     QLKLQYDSVMAALDYEGRAALAVSAVIANLPPVAEAVAKGERDPLMTLLQAPLAAIKP
                     QGMPLISFQTPPATNFFRIHEPKIFGDDISARRKTVVESNQTGKPIVGVEPGRDSLGV
                     FAMTPIMRDGKSIGVVDIGVSFGKPFVDNAKLRFGVDLAVYRFDGKAFTSIASTLGDG
                     SVATQDELKSVFDGNVLRRDATLNGHPAALYLGQIKNYAGQPVAVIELVKDTTEYEAA
                     AASSQRNFILGTFVILAGAALLAFLLGRGLSRPLAAITAVMNRLSSGDTDVTILGSDR
                     KDELGTMAVALDVFRRNMIEARTLREAQEAAKYQAELEKKVLQREMADRFESDVKSVV
                     GAVAQATKDMQRVAGEITTSVNGTSERAAAAAAAAEQASASVGTVATATEELASSVTE
                     IGRQVTFSSGVADEAVAKAAKTTEMVKSLAAAAEKIGDVLRLIGAIASQTNLLALNAT
                     IEAARAGDAGRGFAVVASEVKELASQTAKATEEIAGQVTAIQSATADCALAIGGISDT
                     IREISGVATTIASAVEEQGSATREIARSVQRVATGTGEVSLNVAGASQAADQSRALAD
                     NVLVASGELSQHATALFTSVDTFLAGLRNAA"
     gene            6658..7149
                     /locus_tag="VGA15_09355"
     CDS             6658..7149
                     /locus_tag="VGA15_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX9587938.1"
                     /translation="MKRPTTSVGKRSAATATRARSSVRASSPAVDAVFSAYPKPIKAK
                     LLALRRLIFDTAKTTKGVGALQETLKWGQPSYLTAETKSGSTIRIDQVKSAANQYAVY
                     FHCQTDLVATFRELYPTELSYGGNRSILLNAADDIPEPALRHCVALALTYHLNKRKAA
                     RGS"
     gene            complement(7236..>7421)
                     /locus_tag="VGA15_09360"
     CDS             complement(7236..>7421)
                     /locus_tag="VGA15_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587939.1"
                     /translation="AEGVETEAELSCLRAEGCHEGQGFLFSRARPNAEIVGLLKAQRG
                     ADFMVPVDAPEKAVLVA"
BASE COUNT         1572 a         2277 c         2169 g         1403 t
ORIGIN      
        1 gcagatcctg gtcaacaacg agccggtcga tgcgctgtcg atgctggtgc atcgccagcg
       61 cgccgagggc cgcggccgcg ccatggtcga gaagatgaag gaattgatcc cgccgcacat
      121 gttccagatc ccgatccagg cggcgatcgg cggcaaggtg atcgcgcgcg agaccgtgcg
      181 cgcgttgcgc aaggacgtca ccgcgaaatg ctacggcggc gacatcaccc gcaagcgcaa
      241 gcttctggaa aagcagaagg aaggcaagaa gaagatgcgg cagttcggca aggtcgacat
      301 cccgcaggaa gcgtttattg cggcgctgaa ggtggatagc tgaggcgaat gctcaaccgc
      361 taaagcatga tggattaggt ccggtctgag gcttccgtga ccggacaaca gaaatatcga
      421 aaacaacccc atgcaaagta gccgatgact gccggcgcga tgcctgacgc atcctacgaa
      481 accatttgac acgtcgggca aatcagcggc acgacgccat catcgcggcg cgtgcgatcg
      541 gtggggccct tgccaagccc gtcgaagccg gcgctatcca gcctcacgcg caagcgcaaa
      601 cttctcgaga agcagaagga aggcaagaag aagatgcggc agttcggcaa ggtcgatatt
      661 ccgcaggagg cgtttattgc ggcgctgaag gtggatagtt gaactgattt ggtggccaac
      721 taatcagcct tcacaagaag cttcatcggt cgccttcgcc cgaataaaag gagtgtgagg
      781 tacatctttc gtccgttcgc ggctttcgat ccccagttga atttattctc atcggaaaac
      841 gcaattgtat gttctgattt tggatcgatc gtaaaacgat atccaccgtt gtccggtgtc
      901 aaatcggtaa ctgcggctga aaacgcgcct tgccacgcta acgaccagtg tgcgacttgc
      961 accgtccggt cggaaagatt tacgatggta atttcgtcgt cgactccttt ttggcccgta
     1021 aagctgtagg tagtcgagag tttgatgcgg tcgcgccaga gcgtctccca taatttgatt
     1081 agccccaaga ctgtagaaag tccggcgccc caaaccaaaa gccagaattt aaatagttct
     1141 tccgtcataa gagccctatt tgcttagtaa acggtgacac ggggaggtcc aataaactaa
     1201 tcgctctctc gaaaactgta gcggcactag aatatgcgcg atacgagctg agttcggccg
     1261 tgaacgcgtt catgttgagg cctccttcct gacaggacgt ctttggcgct ccattctttc
     1321 gaggaattcc gcgactttac cgctaaccca ttgagctcga acggcgctat tgtaggaatc
     1381 tcgaatctct tcaggcattt cgttgagtgc aactgtgcca tcaaggattc tcttggctag
     1441 cgactgatcg ccgccaacaa gtcgctcagc ctcatccgga cacaccgtat attctgggta
     1501 gtcaattgtt gaaaatcctt cgaaatcgat cactccctcg aagtcatcag cgagaccact
     1561 caaaaatctc gcaaacggat tttccgtcca ctcgtgacgg tactgcacaa agccagcctc
     1621 atctaactgg tagccaaaca agtctcgact ttcgatcgac tcgcgttccg cccgaatttt
     1681 ttcttctagg tcgtcgaagt ctgatttcct cgtgtgctca atttgccgct ctcgatctcg
     1741 agtgacctga taagagcgct ccaactcttc aagacgacta cgacgttgac gaaggctcgc
     1801 ctcagcaacc cagcacagca gaaacggcgc gaactctata acttgccagg ggcgaacgtg
     1861 gtaacgctca tagattaggt agagagcgtt gctcgcgtgt gagctgattt gaatgttgga
     1921 ttgcgacgct gactgatctt gaagtctagt ctctggaatt gggacttcac ccatcaacac
     1981 agggggttca acagcgagcg cccgtgctag ctggcggatg gtgcgaccat gggtattggc
     2041 ctgcttgcct cgctcaagtc gcgaaatcgt acgtttgtcg attttcggat cgcgcccgaa
     2101 cttcgtattc tccgatagcg cttcttgggt ccaccccttc gcttcacgta accgctttag
     2161 tactgaggga tcaattttgg ccataggcag gaatctccat tatttggaga atgccgtcag
     2221 atggcctatt attaaataga caaattatct acatgatcaa atatgcctat gtattttcgt
     2281 ccatcacaag acatttactg gacgagcgcg aattatctat ttgtttctat tgggaaatcc
     2341 gcccttgata agcgcccgta acatgttacg attctcccat ggccacgacc ccaaaaaagg
     2401 caaaacctcg aacaccaaag cagcgcatgg cttcgcgccg cgagcgcctg cgcgcgcagg
     2461 ggctgcggcc ggtacagcat tgggtgccgg atttgcgcga tccgaaggtg ctcgcggaga
     2521 ttcgccgtga gataaagcta atggcgcagc atccggaaaa tgacgcgatc gatgattgga
     2581 tcgagtcggt ctacgattgg gaatcgtgga aataacgcga ggcgatgtcg tgctgatggt
     2641 cgtcccgagc gatcccggga ggccgcgacc tggcatcgtc gttcaaggcg acaagttcaa
     2701 cgccggcctt tcgacaatct tcgtttgtcc ggtctcttcg gatctacagg acggactttc
     2761 gctccgaccg ctgatcgaag ttgcacccga gaacggtctt cgccttcggt cgcagatcat
     2821 gacagacaaa ctagtcgcgc tccgccgcga ccgcattcgg cgggttattg gtcgcatcga
     2881 cgcggaaacg tccgaacacc tcgatcgcac gctgctcgtc gtgctcggac tggccccgtg
     2941 acgaaaatat ggccggcatc tgcatggctc gtatcactcc cccatcttga tcggcggcgg
     3001 cggatgaacc atcatcgcgg catcgccaat cagaacccca aaaagggcga ggaaacgaat
     3061 gaacaagctt cccggtttcg gccttatcga gcgcgcgcgg gcgcttgcgc cactgatcac
     3121 gcgcgaggcc gatgagatcg agcggacgcg gcgtctcacc gagcctgtcg tgtcggccct
     3181 gatcgagaac gggctttatc gcgtgctgct gccgcaaagc cttggtggca ccgaggcgcc
     3241 gcccgaaatc ttcatgcaga tgctggagga agtggcgaag gccgacgctt caacggcgtg
     3301 gtgcctcggc caatgcagcg tctgcgccat gaccgcggcc tatctcgatc ccgacgccgc
     3361 gaaccagatt ttcaatgcgc cgcccggcat tctggcctgg ggtgcgatcg cccacgaggc
     3421 gcaggccgtt cccggcggct accgcgccaa tgcgcgctgg gacttcgcca gcggctcgcg
     3481 tcaggcgagc tggctcggcg cccatgtcca gatcatcgaa gccgatggca cgcggcgttt
     3541 gaagcccaat ggcgcgccgg aagtgcgcac catcctgttt ccgctggcga gcgccacgat
     3601 gtacgacgtc tgggacgtga tcggcctcaa cggcacgggc accgacagct attcggtcga
     3661 caacctgttc attccagaga aattcacggc attgcgtgac gatcccacag cattgcgtga
     3721 aaagggcccg ctctaccggc tcaccaccaa catggtattc ggcctcggct ttgccgcggt
     3781 ctcggtcggg gtcgcgcgcg ccacgctcga cgccgccgtc gatttggcgc gcagcaagac
     3841 gccggccggt ctcaagaaga tgcacgagaa caacgccgtt cagggcgcga tcgggcgcaa
     3901 cgaggccagt ttgcgcgcgg cgcgcgccta tctctatgcg acggccgcag aggtgtggcg
     3961 cgatctgacg cgcaacggaa acttcacgga ggagcatcgc gcagcgcttc gcctggcgtc
     4021 gacctggacc atccatcagg cagcctcggt ggtcgatgcc gcctatcaca tggccggcgc
     4081 cacggcggtg ttcagcgcca acagattcga gcgccgcttc cgcgatatgc acgccatcgc
     4141 gcagcagatc caggcccgcg acacgcatta cgaagacgtc ggcaagatga tcctcaccgc
     4201 cgatcccggc acatcggcaa acaacgccca atagatgacc cgagggcatg agcggcttgg
     4261 cgatcggatc acgagcaatc gaataaatgg agcgcgaccg gccaaaacca cttcccactg
     4321 ttgctgatcg cgctccaggg gccgcgagca tggtagtact gcggaccaac gggttaacct
     4381 caaattgtta aacattatct gggacgttcc gaactctcct ttctgacgga gcttcggtgt
     4441 tcccatgttc aaacttcggt ctatctctgc gcggctgatt ctgacgattt cagtgaccgt
     4501 cgcggtggtc tgcgggatcc tgggaacttt ttcggttgtt caacagcgct cgttgacaag
     4561 gctcgcgctc gaccagcagc tcaagctgca atatgacagc gtcatggccg ccctcgacta
     4621 tgaagggcgc gccgcattgg cggtcagcgc cgtcatcgcc aatctgccgc cggtggcgga
     4681 ggccgtcgcc aaaggcgagc gcgacccgct catgacgctg ctgcaagcgc ccctggcggc
     4741 gatcaagccg cagggcatgc cgttgatcag cttccagacg ccgcccgcca ccaatttctt
     4801 ccgtattcac gagcccaaga tcttcggcga tgacatctcc gcgcgacgca agaccgtggt
     4861 ggagtcgaac cagaccggca agccgattgt tggcgtcgaa cccggccggg attcgctcgg
     4921 cgttttcgcc atgacgccga tcatgcgcga cggcaagagc atcggcgtcg tcgacatcgg
     4981 cgtttccttt ggcaagccat tcgtcgacaa cgccaagctg cgcttcggcg tcgatctggc
     5041 ggtgtatcgc ttcgacggca aggcgtttac cagtatcgca tcgacgctag gtgacggctc
     5101 ggtcgccacc caggacgaac ttaaaagcgt atttgatggc aacgtcctgc gtcgcgacgc
     5161 cacgctcaac ggccatccgg cggcgctcta tctcggtcag atcaagaact atgccggaca
     5221 acccgtcgcg gtgatcgaac tggtcaagga caccaccgaa tacgaggccg cggcagcgag
     5281 ctctcagcgc aatttcattc tcggcacgtt cgtgatcctt gccggcgccg cgctgctggc
     5341 gttcctgctc ggccgcggcc tgtcgcgccc gctggcggcg atcaccgccg tgatgaaccg
     5401 cctctcgagt ggcgataccg acgtaactat actcggcagc gaccgaaaag acgagctcgg
     5461 caccatggcc gtggccctcg acgtgttccg ccgcaacatg atcgaggcac ggacgctgcg
     5521 cgaggcgcag gaagctgcca aatatcaggc cgaactggag aagaaggtgc tgcaacgcga
     5581 aatggccgac cgcttcgaat ccgacgtcaa gagcgtggtc ggcgccgtcg cgcaagcgac
     5641 aaaggacatg cagcgcgtgg ccggcgagat caccacgagc gtcaacggaa cttcggaacg
     5701 ggccgccgcg gcggccgccg ctgccgagca ggcctcggcc agtgtcggca cggtcgccac
     5761 cgccaccgag gaacttgcct cttccgtgac cgagatcggc cgacaggtta cattttccag
     5821 cggcgttgcc gacgaggccg tggccaaggc cgcaaagacc accgaaatgg tcaagagcct
     5881 cgccgccgcc gccgaaaaga tcggcgatgt gctgcgcctg atcggcgcga tcgccagcca
     5941 gaccaatctg ctggcgctca acgcgaccat tgaggcggcg cgggccggcg atgccggcag
     6001 gggctttgcc gttgtcgcat ccgaggtcaa ggaactcgcc agtcagaccg cgaaggcaac
     6061 cgaggagatc gccgggcagg tgaccgccat acaatccgcc accgccgatt gtgctttggc
     6121 gatcggcggc atcagcgaca ccatccggga gatcagcggg gtcgccacca ccatcgcttc
     6181 ggctgtcgaa gagcagggct cggcgacacg cgagattgcg cgaagcgtgc aacgggtcgc
     6241 cacgggcacc ggcgaagttt cgctcaatgt cgcgggcgca agccaggcgg ccgaccagtc
     6301 gcgcgccctg gccgacaacg tcctggtcgc gtccggcgag ctcagccagc acgccaccgc
     6361 gctgtttacg agcgtcgata ccttcctcgc cggcctgcgc aacgccgcct aggcgtgcgc
     6421 ggtttcaccg gcgctgaaat tcacggctcg gcgaaagctc tcgggaagct ggtccgggcc
     6481 aatcaactgg acgattcaac aactaaaagc cgccactacg cgcaagcgaa atgcgacggc
     6541 aaagtcgcgc aaaaccgggc aaacgaaata gcactccgga aaatctggct ttcggtcctc
     6601 gggcagctgc tctaatctgt ccgccagact gcatcggcaa tgtacggacg gaccgcgatg
     6661 aagcgcccga caacgagcgt tggaaaacgg agtgctgcaa cggcaacccg cgccaggtct
     6721 tctgtcaggg cttcaagtcc cgcggtcgac gcggtcttca gcgcctatcc aaagccgatc
     6781 aaggcaaaat tactggcgtt gcgccggctg attttcgaca ccgcaaagac caccaaaggc
     6841 gtcggcgcgc tgcaggaaac cctgaaatgg ggacagccga gctatctcac cgcagagacg
     6901 aaaagcggca gcacgattcg aatcgaccag gtgaaatccg cagccaacca atacgcggtc
     6961 tatttccatt gccagaccga tctggtcgcg accttccgcg aactgtaccc gacagagctg
     7021 agctatggcg gcaaccgcag cattcttctg aatgcggcgg atgacattcc tgaaccggca
     7081 ctgcgtcact gcgtggcgct ggcactgacc tatcatctga acaagcgcaa ggcggcgcgg
     7141 ggctcgtagc ccggatgaag cgaagcgcaa tccggggtcg gcgccaatta atccccggat
     7201 tgcgcttcgc ttcatccggg ctacgctcca gctcttcagg ccacgagcac ggctttttcg
     7261 ggggcgtcca ccggcaccat gaaatccgcg ccgcgctgcg ccttcagcaa cccgacgatc
     7321 tcggcgttgg gccgcgcgcg gctgaacaga aagccctggc cctcgtggca cccctcggcg
     7381 cgcaggcagc tcaactcggc ttcggtctcg acgccttcgg c
//
LOCUS       DASWGM010000325         4640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2527230,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000325 DASWGM010000000
VERSION     DASWGM010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527230"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VGA15_09365"
     CDS             <1..237
                     /locus_tag="VGA15_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9587940.1"
                     /translation="CVVAGMGIALIPKSVLATFPERKRLSIHLLARTENRAQTVLICR
                     KGAGSANVKALQEILQGRKLASSSKIRITGMELS"
     gene            311..2184
                     /locus_tag="VGA15_09370"
                     /pseudo
     CDS             311..2184
                     /locus_tag="VGA15_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
     gene            complement(2336..3115)
                     /locus_tag="VGA15_09375"
     CDS             complement(2336..3115)
                     /locus_tag="VGA15_09375"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9587941.1"
                     /translation="MSTFEHIIVESKGAVGIITLNRPKMLNALSFGVFGEIAAAVDDL
                     EADDGIGCILLAGGEKAFAAGADIKEMQPKTFIDMFSSDFIGIGADRVARCRKPTIAA
                     VSGYALGGGCELAMMCDIIIAADTAKFGQPEITLGTIPGIGGTQRLTRAIGKSKAMDL
                     CLTGRMMDAAEAERSGLVSRVVPTDKLMGEALAVAEKIASMSRPAAEMAKSAINRAFE
                     TPLTEGLNVERDLFHATFALEDRSEGMAAFIEKRKPVNKNR"
     gene            complement(3262..4002)
                     /locus_tag="VGA15_09380"
     CDS             complement(3262..4002)
                     /locus_tag="VGA15_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587942.1"
                     /translation="MTIKASGRSVSILSLLILATGLFVCFAGPLPAAAGADDNAAADS
                     QSANSEPNGAAAEPARHHKRTRHVSRHWRSYAHHKSGKVALKSATARKDDAIDSDIST
                     AIPPSVANANALLATADTPDNNARAMTARANDMLQVAAQDNVPQAAAEKPADAQPAAE
                     TQVLPPDQLNDVDRTLPASAASAPAPATTSADAPAQAMASADSPDEPVVANSDDSSSV
                     DRTSLIGKIFMACGGVLTLASAVRMFMA"
     gene            4285..>4640
                     /locus_tag="VGA15_09385"
     CDS             4285..>4640
                     /locus_tag="VGA15_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX9587943.1"
                     /translation="MRELDEEWTSLMRSAISGDSAAYHRLLKAVAPVLRAAARRGLAR
                     AGQPVDQSEDIVQDILLAVHLKRHTWDVNAPFAPWLFAIARNKLIDALRRRGRRVFVN
                     IDDFAETIPSEPAAETA"
BASE COUNT          822 a         1434 c         1472 g          912 t
ORIGIN      
        1 tgcgtcgttg cgggcatggg gatcgccctg atcccgaaga gcgtcctggc gacatttccg
       61 gagagaaagc gtttgagcat tcatcttctg gctcgaacgg aaaatcgtgc gcagaccgtt
      121 ctgatctgcc gcaagggagc aggttcggcg aacgtcaagg ctttgcagga gatattgcaa
      181 ggccgcaagc tcgcatcttc atcaaaaatc cggataacgg gtatggaatt atcatagttt
      241 cgataggcaa cgttcgattc tcgggggatt gccggatgtt ccggttcgcg ccacgccaga
      301 cggaatattc atgccgaaaa agttcaccga ccccgatgcg attgcggagc atatcatccg
      361 cgatgtcggc acccatctgg tggtcggcct gccgctcggg ctcggcaagg ccaaccacat
      421 cgccaacgcg ctctacgcgc gcgccgccgc cgaccggacg atcagcctga ccttcttctc
      481 cgcgctgacg ctggaaaagc caaagccttc aaatctgctt gagcggcgct tcatcgcgcc
      541 ggtgatcgag cgcctgttcg gctcctatcc cgatctcgct tatgccgacg cgctgcacgc
      601 gggtgcgctg ccgcccaaca tacaggtgat cgagtttttc tttctggcgg gaagatggct
      661 gcgcgtgccg ttcgcgcaac aacattatat ttccgcgaat tatacccacg cttcgtccta
      721 tctgctggcg cgcgggctca atgtcgtcac ccaactggtg gccaagcgca ttgtcgatgg
      781 cgtgacgcgc tacagcctga gctgcaacac cgacaccacg ctggacatct tgcgcgcccg
      841 cgcagaaaac cgcgcgtcgt tcaaactggt cggccaggtc aattccgaac tgccgttcat
      901 gccgggcgcc ggcgaccttc cggcggatga attcagcgcg gtgctcgata gccccgagac
      961 cgattttccg ctgttcgcgc cgccgtccga gccgatcacc gacaccaaat atgccgtcgg
     1021 ccttcacgcc gcgcgcctcg tgcgcgacgg cggcacgctg cagatcggca tcggacaagt
     1081 cggcgacgcg ttggcgcagg gcctcatcgt gcgccaccgc gacaacccgc ggtttcatgc
     1141 gatcatgaag cggctggcgc cggacgcgcc ggttgtcgcc gagaacaacg gaccgttcga
     1201 acagggtctc tatggcgtca gcgaaatgct gttcgaggca tttctcggcc tgatcgacgc
     1261 cggcattctc aaacgcgaag tcgacggcgt gttgctgcac ggcgcgttct ttctcggacc
     1321 gaaatcgttc tatcgcgcgc tgcgcgagat ggcgccgcag caactcgcgc gcatccggat
     1381 gatgccggta tccttcacca acgagctcta tggcgatgag gacttgaagc ggcgcgcccg
     1441 catcgacgcc cgcttcgtca acaatgcgat gatggcgacg ttgatggggg cggcgatttc
     1501 cgatggcctt gaaaatggac aggtcgtaag cggtgtcggc ggccagtaca atttcgtggc
     1561 gcaagcgttc gcgctgcagg gtgcgcgttc gattctcacg gtcgaagcga cccggcaggc
     1621 cggcgccaaa gcgcaatcga acatccgctg gagctatggc catgagacca ttccgcggca
     1681 tctgcgcgat gtcttcatca ccgaatatgg tgtcgccgat gtcaggggaa aatccgacgc
     1741 cgatgtgatc gcggcgatgc tggccgttgc ggattcgcgc tttcaggccg aactcgcgcg
     1801 gcaggccaag gacgccggca agctgccgaa ggagtttgaa ataccggcgg cgcaccgcga
     1861 caatcacccg gatcggattg cagctgcgct gaagcctgcg cgcgaggccg gcctgctgcc
     1921 gcagtttccg ttcggcagcg attttaccga caccgagcag cggcttattc cggccttgca
     1981 attgttgcag gacgcgcaac gcgcgccgca gcgcttttgc ccgggatgct gtggcaaggt
     2041 ttgacgcgca ccccggatgc tgccgacagc gaaggcctgg cgcgactcgg cctcgatgtg
     2101 cccaagacat tgtccgaacg cgcctaccgc gcgctggtga gtgccgcgct ggcgaggagc
     2161 agggcgggcc gtacgtcatc ttaacgacat ctgccacaca ctcgtgtccc ggacgcggtg
     2221 cagcgttctt tacggtgcac cgcagagccg ggacccatct aaatcgggtg ccgaaatatg
     2281 ggccccggct cagcagcgca ccgctacgcg ctgcgcagcg tccggggcac gagatctacc
     2341 tgttcttgtt caccggcttg cgcttctcga taaacgccgc catgccttcg gagcgatcct
     2401 ccagcgcgaa ggttgcgtga aacaggtcgc gctcgacgtt aagtccctcg gttagcggcg
     2461 tctcgaacgc gcggttgatg gcgcttttcg ccatctcggc cgcggggcgc gacatcgagg
     2521 cgatcttttc cgcgaccgcc agcgcttcac ccatcaattt gtccgtgggc acgacgcggc
     2581 tgaccaggcc cgagcgctcc gcttcggcgg catccatcat tcgcccagtg agacacaggt
     2641 ccatcgcctt ggatttgccg atcgcacggg tcagccgctg cgttccaccg atgccgggga
     2701 tggtgccgag cgtgatttct ggctggccga atttcgcggt gtcggcggcg atgatgatgt
     2761 cgcacatcat cgccagttcg cagccgccgc cgagcgcata gccgctgacg gcggcaatgg
     2821 tcggtttgcg gcagcgggcg acgcggtcgg cgccaatccc gatgaaatcg ctggaaaaca
     2881 tgtcgatgaa ggttttcggc tgcatttcct tgatgtcggc gcccgcggcg aaagcctttt
     2941 cgccgccggc cagcaggatg catccgattc cgtcgtcggc ttcgagatca tcgaccgccg
     3001 cggcgatctc gccaaacacg ccgaacgaca gcgcattgag catcttcggg cgattgagcg
     3061 tgataatgcc gaccgcgcct ttgctttcga caatgatatg ttcgaacgtg ctcatggttc
     3121 accccgcgct tgatttcgcg caatgtgccc gctggcgggg tgagcttcaa gtggggcgaa
     3181 tctggagcca aagtgagcgc gactgcgtca cccccaggtg aatgcaactg cgttcacccg
     3241 gggatgacgg tcatcgtcag ctcacgccat gaacatgcgt acggccgagg ccagcgtcag
     3301 aactccgccg caggccatga agatcttccc gatcagggag gtccggtcca cgctcgaact
     3361 gtcgtcgcta ttggccacca cgggttcgtc gggcgaatcg gccgaggcca tcgcctgggc
     3421 cggcgcgtcg gccgatgtcg ttgccggggc cggcgcggac gccgcgcttg caggcagcgt
     3481 gcgatcgaca tcgttgagct gatcgggggg aagaacctgg gtctcggcgg caggctgtgc
     3541 gtcagccggt ttttctgccg cggcctgcgg aacattgtcc tgcgccgcca cctgcagcat
     3601 atcgttggcc cgcgcggtca tggccctggc gttgttgtcc ggcgtgtcgg cggttgccag
     3661 cagcgcattg gcgttggcca ccgacggcgg aatcgcggtt gaaatgtcgc tatcgattgc
     3721 gtcgtctttc ctggcggttg cggatttcag cgccaccttg ccggatttat ggtgcgcgta
     3781 gcttctccag tggcgcgaga catgcctggt gcgtttgtgg tgcctggccg gttccgcggc
     3841 ggcgccgttc ggttccgaat ttgccgattg cgaatctgct gccgcgttgt cgtccgcgcc
     3901 cgccgccgct ggtaatggcc ctgcgaaaca cacaaagagc cctgtcgcca gtatcaacaa
     3961 cgagagtatc gacaccgagc gcccgctggc tttgatggtc atttgagaat ctccccattt
     4021 gccagccccg aaaacgcgta tgagagctgc cgggatgtcg gcatcggggc agaaaaaggg
     4081 aatcttcggg cgagtgctgg cgaaagcagg gcaggagtat tgcggtaatc tttttggaat
     4141 ggatggggcg ggtgcctgcg ggaccgatcg atgttatgag cctcccagtg cctgactccg
     4201 aagatcccga tcacgacctc gtgattgaaa gccttcagac gtaacaaatt gaaagatcga
     4261 ccgaattgca gtgtgggagc gcgcgtgcgc gaacttgacg aagaatggac cagcctgatg
     4321 cggtcggcca tttccgggga cagtgctgcg tatcaccgtc tgctcaaggc cgttgcgccg
     4381 gtcttgagag ccgcggcgcg gcgcggtctg gcgcgggcgg ggcaaccggt cgatcaatcc
     4441 gaggacatcg tgcaggacat cttgttggcg gtgcatctga agcggcatac ctgggacgtc
     4501 aatgcgccgt tcgcgccatg gctgtttgcg atcgctcgca acaaattgat cgatgcgctg
     4561 cgccgtcgcg gccggcgggt tttcgtcaat atcgacgatt tcgccgaaac gatcccaagc
     4621 gaacccgccg cggagaccgc
//
LOCUS       DASWGM010000326         5224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_33774,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000326 DASWGM010000000
VERSION     DASWGM010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33774"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGA15_09390"
     CDS             complement(<1..528)
                     /locus_tag="VGA15_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587944.1"
                     /translation="MRNPVKERVSLVTKHDFDSLPTDSEIEVRPARYSELAIVADMGH
                     RLVPGVQISELMLGDYLTFDSECILTFGRHEKLLGAMAFLYLNSRGHDALMLGEISLA
                     HPDIGMLAGRNDAVSAIYIWAIAANGRGIAGLGKAAAHLRAPRYVNANCFAQPSSRAG
                     RDLLIATGFRQTPSFQ"
     gene            581..1642
                     /locus_tag="VGA15_09395"
     CDS             581..1642
                     /locus_tag="VGA15_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9587945.1"
                     /translation="MQQLLHRGAAVTQHRNEEILDASWDDLKSFLVCAKHKSFRNAAE
                     MLGLTSTTLMRRIDRLEERIGCKLFLRDQSGLTLSEEGLAMIGDVTEMERHAFNIFRR
                     VSHSTNDGSGTVRVSVTEGPGNFWILPRLIDFQKTYRKITVDLHCAMEHADVARMESD
                     IAIQLEPPTNPDLIVAKIGRLHIYPFVSEGYRKLYGVPASLSELKNHRIIKQNAPQVD
                     DTAYARILGLTSLEGVVGIKTNSSISVLYAVERGAGVGFLPTSSIALGAPLIAVDMGA
                     SHHADLWLAYHKEFRNSDRHKIVIAWLKQIFDPKTYPCFRDEFIHPNDLVPLMTGPRS
                     NFGLEGYAAAGLAQGREFD"
     gene            complement(1747..2886)
                     /locus_tag="VGA15_09400"
     CDS             complement(1747..2886)
                     /locus_tag="VGA15_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEX9587946.1"
                     /translation="MCCRSRLSQAKETGTFRRLVVAAAIAFVLAVATASSSVQAQTLT
                     PNGSLAPRPTPSPTPTAINSDLSAGAAVTNLGSNFLERLGNQATNGFGGALRSNPAGG
                     GASESTDAPRFRTWAEAYGISARTRAQGDFVGDHRQTWGGVAGFGARVAPGVNVGVSV
                     DQSRTAIDVPLALQSATLDLTQLGFNASVDKGPWTWAVALVHGFGKINSSRDTGFGTA
                     SAGYNAHIDGALTELSYYWDIDQSRVVPKAAFEYVRAVTGSLQEFGGLDPVMASGATA
                     ERSRLLMGAEVGHYWILGRKIFDLSAYGKFVDNVAQDFSAVTVSLGTQSITVQGIGES
                     RYGADAGASASLSLTNTARLYLNYDGKFRAAAQSHQGTVGVELKW"
     gene            3261..3464
                     /locus_tag="VGA15_09405"
     CDS             3261..3464
                     /locus_tag="VGA15_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9587947.1"
                     /translation="MQKGTVKWFNPTKGYGFIKPMAGDKDVFVHISAVERAGLSTLNE
                     NQVVEYDLVENRGKSSAENLKVS"
     gene            complement(3553..4479)
                     /locus_tag="VGA15_09410"
     CDS             complement(3553..4479)
                     /locus_tag="VGA15_09410"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9587948.1"
                     /translation="MAKIAVVGCGAMGSVYAALMVDAGHEVHAVTLWPDHAEAMASRG
                     LRCEGASGDRTVPIHASTTTGGIGPCDLVIIATKSFDVEAAAHSCLPLLGPETIVQTI
                     QNGLGSPEIAAPILGADRIAVGVVGGFGASIRAPGHAHHNGMEMIRFGAFAGLPKQLL
                     QASAKVWESAGFKVALFDDIKQMVWEKLIMNVAFSGTSCTTGLTVGEILGDADAWSVA
                     RGCAEEAIAVAKAANVRLNVGDPIEHIRKLGGKIPDARPSMLLDYNAGRRCEVDAING
                     AIPRVGKPLGVATPINDAVVGIIRARERRMLA"
     gene            complement(4516..5169)
                     /gene="purN"
                     /locus_tag="VGA15_09415"
     CDS             complement(4516..5169)
                     /gene="purN"
                     /locus_tag="VGA15_09415"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX9587949.1"
                     /translation="MKRRVAILISGRGSNMAALIQAATAEDFPAEIVVVISNRADAGG
                     LERAKASGIPTLSIESKPFGKDRAAFEAVLQSALDQHKVELICLGGFMRLFTAEFVQR
                     WYGRMLNIHPSLLPSFPGLDPQGQALRAGVKISGATVHFVIPETDAGPILMQGAVAVR
                     DDDTPATLAVRILAIEHRIYPDALRLVASDKIRLEGGICKTAGRGVSDDVLISPTVT"
BASE COUNT         1063 a         1596 c         1564 g         1001 t
ORIGIN      
        1 ctggaagctc ggcgtctgcc tgaagcccgt ggcgatcagc aaatcgcgac cggcccttga
       61 cgatggttgg gcgaagcaat tggcattgac ataacgtggc gctctcaaat gagcagcagc
      121 tttgcctaag ccggcaatac cgcgaccgtt tgccgcaatt gcccagatgt agatcgcaga
      181 caccgcatca tttctcccgg ccagcattcc gatgtccggg tgcgccagag aaatctcgcc
      241 gagcatcaga gcatcgtgtc cgcggctgtt gagatagaga aacgccattg cgccaagcaa
      301 cttttcgtgc cggccgaacg tcaggatgca ttcagaatcg aaagtgaggt agtcgccaag
      361 catcagttcc gagatctgaa ctcccggcac caggcgatgg cccatatccg caacgatcgc
      421 aagttcggag tatcgcgcgg gacgcacctc aatctcagag tcggtcggca gcgaatcgaa
      481 atcgtgcttt gtgaccaaag acaccctttc cttaaccgga ttgcgcatct atcgttactc
      541 ctgcccgcag caagcaccat accgggtggg gatttagggt atgcagcaac tattgcaccg
      601 gggtgctgcg gtgacgcagc accgaaacga agagattctg gatgcgtcct gggacgatct
      661 gaaatcgttt ctcgtctgtg cgaagcacaa gagttttcgt aacgctgccg aaatgcttgg
      721 tctgacgagc acgaccctga tgcgaaggat cgaccggctt gaggaacgga tcggctgcaa
      781 gttgttcctg cgcgatcaaa gcggcttgac gctgagcgaa gaagggctgg cgatgatcgg
      841 cgacgtgaca gagatggaac gtcacgcctt caatattttt cggcgcgtgt cgcactcaac
      901 gaacgacggt tccggtaccg tgcgggtttc agtgaccgag gggccgggca atttctggat
      961 tctgccgcgg ctgatcgatt tccagaaaac ctaccgcaag atcacggtcg acctgcactg
     1021 cgccatggaa cacgcggatg tcgcgcggat ggaatccgat atcgccattc agctcgaacc
     1081 gccgacaaat cccgacctta tcgtcgcgaa aatcggcaga cttcacatct atcctttcgt
     1141 gtccgaagga taccggaagc tctacggtgt tcctgcatcg ctgtccgagc tcaagaacca
     1201 ccgaattatc aagcaaaacg cgccgcaggt ggacgatacc gcatatgctc gaattttggg
     1261 actgacctcg ctggaaggcg ttgtcggtat caagacgaac tccagcatca gcgttctcta
     1321 tgcggtggaa agaggcgccg gcgtaggttt tctgccaacc tcgtcgatcg ccctgggtgc
     1381 acctttgatc gcggtcgata tgggcgccag tcatcatgcc gatctttggc tcgcctatca
     1441 caaagagttt cgcaactccg atcgacacaa aatcgtcatt gcctggctca aacagatatt
     1501 cgatcccaag acctacccgt gctttcggga tgaatttatt catccaaacg atctggtgcc
     1561 gttgatgacg gggccgagaa gcaacttcgg cctggaggga tacgccgccg ccggattggc
     1621 tcaggggcgc gagttcgatt gatccggggt cggcgctgca gggctgatcc cagtttcgct
     1681 ggtatcccac ccagcgggtc gtggccggag ggcacggttt tcggaactag gctccaaccc
     1741 gccgcattac cacttcagct cgacgccgac ggtcccctga tgcgactgcg ccgcggcgcg
     1801 aaatttgccg tcatagttga gatagaggcg agccgtattg gtcaggctga gcgacgcgga
     1861 ggcgccggca tctgcgccat atctgctttc gccgatgccc tgcaccgtga tgctctgggt
     1921 gccaagactg accgtgaccg cactgaaatc ctgcgcgaca ttgtcgacga acttgccgta
     1981 ggcggacaga tcgaaaatct ttcgacccaa gatccagtaa tgcccgacct ccgcgcccat
     2041 cagaagccgc gaacgctccg cggtcgcgcc gctcgccatg accggatcaa ggccgccgaa
     2101 ctcctgcagc gatccggtca cggcgcgcac atattcgaac gcggccttcg gcacgacgcg
     2161 gctctggtcg atgtcccagt agtagctgag ctcggtcaac gcgccgtcga tgtgggcgtt
     2221 gtaaccggcg ctggcggtgc caaagccggt gtcccggctt gaattgattt tcccaaaacc
     2281 gtggaccagc gcaacggccc aggtccacgg acccttgtcg accgaggcgt tgaagccgag
     2341 ctgggtgaga tcgagcgtgg ccgactgcag cgccagcgga acgtcgatcg cggttcggct
     2401 ttggtcgacc gacacgccga cgttaacgcc aggcgcaacg cgcgcgccga accctgcgac
     2461 gccgccccag gtctgccgat gatcgccgac gaaatcgccc tgcgcgcggg ttctggccga
     2521 gattccgtag gcctctgccc aggtccggaa gcgcggcgcg tcggtcgatt cggaggcacc
     2581 gccaccggcc ggattgctcc gcaaggcgcc gccgaaaccg ttcgtcgcct ggtttccaag
     2641 gcgctccagg aaattgctgc cgagattcgt cacggccgcg ccagccgaca gatcggaatt
     2701 gatggccgtt ggcgtcggcg acggagttgg cctgggagcc agcgaaccgt tgggcgtcag
     2761 cgtttgtgcc tggacggacg acgacgccgt ggcaacggcg aggacgaaag cgatcgcagc
     2821 agccaccaca agcctgcgaa aggtcccggt ctccttcgct tgcgaaagcc gcgatcggca
     2881 gcacatgaac gctatttcca aaaagcagcg ccgactcggc aagaaatgca aaataccacg
     2941 ggctcgatag tcgatttgcg ccgagttccc cggaagcgtc aaccggcctt ggtccttaaa
     3001 aatgggcgat tgccgcacat tgttgttgca atgccacagg tgattcgggc gttgtcattg
     3061 atgactagat ttcctgagct gtgccaaaag cttgctaatg cgcaaaattc gtgttgcaat
     3121 atccggcaca atcggatcta ggccgttcga ctgtgcgttt cgcgactagg aaattcagca
     3181 gacatccgga agcccgcttg tggcgtggca cgcaaaatgc gtggccgcgt cttttttatg
     3241 tctagactag gagacaggcg atgcaaaaag gcaccgtcaa gtggttcaac ccgacgaagg
     3301 gttatgggtt catcaagccg atggccggcg ataaggacgt gttcgtccat atctcggcgg
     3361 tcgagcgtgc ggggctctca accctcaacg agaatcaggt tgtcgaatat gaccttgtgg
     3421 aaaatcgcgg caaaagctcc gcggaaaatc tcaaggtctc ttaaacttcg tcacgatccg
     3481 cgcaagcaat caggatgtac ccccggcctt cgccggggga atttgttttc atgttgtcgg
     3541 tttcgtgacc catcacgcga gcatccggcg ttcgcgagcc ctgatgatgc cgaccacggc
     3601 gtcattgatt ggcgtcgcca ccccgagcgg cttgcccacc cgcgggatcg cgccgttgat
     3661 ggcgtccacc tcgcatcggc gcccggcatt atagtccagc agcatcgagg gacgcgcatc
     3721 gggaattttg ccaccgagtt tgcggatgtg ctcgatcgga tcgccgacgt tgagcctgac
     3781 attggcggcc ttggctaccg cgatggcttc ctcggcgcag ccccgcgcca cgctccaggc
     3841 atcggcgtcg ccgaggattt cgccgaccgt cagcccggtg gtgcatgacg tgcccgagaa
     3901 ggcgacgttc atgatcagct tttcccagac catctgcttg atgtcgtcga acagcgcgac
     3961 cttgaagccg gccgactccc agaccttggc ggaggcctgc agcaactgct tcggcagccc
     4021 ggcaaatgcg ccgaaccgga tcatctccat tccattatgg tgggcatggc ccggcgccct
     4081 tatcgacgcg ccgaaacctc cgacgacgcc gacggcaatc cgatccgcgc ccaggatcgg
     4141 tgctgcgatc tccggcgagc ccaagccgtt ctggatggtc tgcacgattg tctcggggcc
     4201 gagcagcgga aggcaggaat gcgccgccgc ctcgacgtcg aacgacttgg tggcaatgat
     4261 gacgagatcg cagggcccga ttcccccggt cgtggtggag gcatgaatgg gcaccgtgcg
     4321 gtcgccgctg gcgccttcgc aacgcaggcc cctggaagcc atcgcctcgg cgtgatcggg
     4381 ccacaacgtg accgcgtgaa cctcgtgacc ggcgtcaacc atgagcgcgg catagaccga
     4441 ccccatcgcg ccacagccga caaccgcaat tttcgccatt ttactctccg tgcaagcagc
     4501 ccgacggcaa caagtctagg tgacggtggg cgaaatcaag acgtcgtcgg aaacgccacg
     4561 gcctgcggtc ttgcagatac cgccttcgag ccggatttta tcactggcga ccagccgcag
     4621 tgcgtcgggg tagatccggt gttcgatcgc aaggatccgc accgccagcg ttgccggcgt
     4681 gtcgtcgtcg cgaaccgcga ccgcgccttg catcagaatc ggaccggcat cggtttccgg
     4741 aatgacgaaa tgcacggtcg cgccggagat tttcacccca gcgcgcaacg cctggccttg
     4801 cggatcaagc ccgggaaacg acggcagcag cgagggatga atgttgagca tccggccgta
     4861 ccagcgctgc acgaattcgg cggtgaacaa ccgcatgaag cctccgaggc aaatcagctc
     4921 gaccttgtgc tggtccagcg ccgactgcaa aaccgcctcg aacgccgcgc ggtccttgcc
     4981 gaacggcttg ctttcgattg acaaggtcgg gatgccgctg gcctttgccc tttcgagacc
     5041 gccggcatcg gcgcgattgg agatcacgac gacgatctcg gccgggaaat cctctgccgt
     5101 cgcggcctgg atcagcgcgg ccatgttcga tccccgcccg gaaatcagga ttgcgacacg
     5161 gcgtttcatg gcctcaccat gcgagatcga tctcaccatg tgaggtcgag atgaccatta
     5221 taga
//
LOCUS       DASWGM010000327         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2108679,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000327 DASWGM010000000
VERSION     DASWGM010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108679"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="VGA15_09420"
     CDS             complement(<1..1581)
                     /locus_tag="VGA15_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9587950.1"
                     /translation="MTIKFNRIGNKLGLASLVSILLSVGMVANQSLTEQSITDANQRA
                     DVDQEIADNALKAESEMRNMQLAVRGVRLARTKPDVEKLRGEFSAAQAAQAKWLDAAI
                     SLALLPETRELFTKLRASTQKYAEGADEQAQLHTKLLDLNGKRITLSAEWDSRIEKLY
                     ALLGKSEAANRSAIEANLRDSDGALNAVRAEGWRFFSTGEASQKEKLVRAATMLSESL
                     KRARGFADDKALRDGIESLEPLVKEFVEASAQAIDADEAKNAISKTRTIPLANQVADL
                     LRTSVPVAQKAAADARANAADKLLQAGRVNLGFGTVIIVVLLGSMIFSFMAIARPMTR
                     LNAAMGRMAAGALDVVIPGAERGDEIGDIAKTISVIRANAEQTARDEAETKVNQDRMV
                     AKQRKADMTRLADDFENAVGEIVETVSSASTELEASATTLTSTAERAQELATMVAAAS
                     EEASTNVQSVASATEEMSSSVNEISRQVQDSARMASEAVDQARATNDRVGELSKAAAR
                     IGDVVELINTIAGQTNLLA"
     gene            complement(1909..2142)
                     /locus_tag="VGA15_09425"
     CDS             complement(1909..2142)
                     /locus_tag="VGA15_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587951.1"
                     /translation="MSLYFFRVSHGRYSGAPDHGSEFESREDAWSEMTKVCGNLVGGV
                     SRSLKQNGEWQMELLDEAKKPVFRIRLVAESFG"
     gene            complement(2321..2914)
                     /locus_tag="VGA15_09430"
     CDS             complement(2321..2914)
                     /locus_tag="VGA15_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587952.1"
                     /translation="MLARFVLGALTASAVMAPALAGQIMNGEEARKFVSGKVFAFTCF
                     DGTRGAGRVLDDGSAMGAVQFSGSGPVRHMRLPGNTLQVRGQSVCASIKGLPFEPCFN
                     LDKKDERNFRGSVSGMGFAYCDFRHQGAAQMLMARAVARPRVLHPPEEQTRSADASRT
                     TGSVRVETPPVEHSAIEPVKSEPAKSENVLELRRSTD"
BASE COUNT          552 a          935 c          899 g          603 t
ORIGIN      
        1 cgccaggagg ttggtctgtc ctgcgatggt gttgatcaac tcgaccacgt cgccgatccg
       61 ggccgccgcc ttcgacaatt caccgacgcg atcgttggtg gcgcgggcct gatcgaccgc
      121 ctcgctggcc atccgggccg aatcctgaac ctgacggctg atctcgttga cggatgagga
      181 catctcctcg gttgccgacg ccaccgactg tacgttggtg gaggcttcct cggacgccgc
      241 cgcgaccatg gtggcaagct cttgcgcacg ctccgcggtc gaggtgagcg tggtggcgga
      301 ggcttccagc tcagtcgaag ccgaagacac ggtttcgacg atttcgccga ccgcattttc
      361 gaaatcgtcg gcgagcctgg tcatatcagc cttgcgttgt ttggccacca ttcgatcctg
      421 attgaccttg gtttccgctt catcccgggc cgtctgttcg gcgttcgcgc gaatgacgct
      481 gatagtcttg gcgatgtcac cgatctcgtc gccccgctcg gcaccgggga tgacaacgtc
      541 gagcgcgcct gcggccatcc ttcccatcgc ggcgttcagc ctggtcatcg gccgggcgat
      601 cgccatgaag gagaagatca tcgatccgag cagcaccacg atgatcaccg tgccgaaccc
      661 cagattgacg cggccggcct gaagcaattt atccgccgcg ttcgcccgcg cgtcggcggc
      721 cgctttttgc gcgacgggca cgctcgtccg cagcaggtcg gcgacctgat tggcgagcgg
      781 aatcgtgcgc gtcttgctga tggcgttctt cgcctcatcg gcatcgatcg cctgcgcgct
      841 ggcttcgacg aactccttga ccagcggttc gaggctctcg ataccgtcgc gcagggcttt
      901 atcgtcggca aatccgcggg cgcgcttcaa tgactcggac agcatcgtgg cggcgcgaac
      961 aagcttttcc ttttgactgg cttctccggt cgagaagaac cgccagcctt cggcgcggac
     1021 ggcattcagc gcgccatcgg aatctcgcaa attcgcctct atggcgctgc ggttcgcagc
     1081 ttcgcttttg ccaagcaacg catacagctt ctcgatgcgg ctgtcccact cggcggacag
     1141 cgtgatgcgc ttgccattga gatcgagaag cttggtgtga agctgggcct gttcgtcggc
     1201 gccttcggcg tatttctgtg tcgaggccct gagcttggtg aatagctccc gcgtctccgg
     1261 caacaaagcc agcgagatgg cggcatcgag ccatttcgcc tgcgccgctt gcgccgccga
     1321 aaactcgccc ctgagcttct ccacgtcagg ctttgtcctc gcaaggcgca cgccgcgaac
     1381 cgcgagttgc atgtttcgca tttcggattc ggccttgagc gcgttatcgg caatctcctg
     1441 atccacgtca gcgcgctgat tggcatcggt gatggactgc tccgtcaagc tctggttggc
     1501 caccatgccg accgacagca aaatgctgac gaggctggca aggccgagct tgttgccgat
     1561 gcggttgaac ttgatggtca tcgcaggatt gcttttcgat ggtgggcaat ggtggcggag
     1621 acggaatcgc gggaaatctg cgtttgaacg acgaacgctc tccccggtaa ttattaccgt
     1681 taacggttcc atattcgttt aggcgcggca ccgtagaact acggagaatt gcgtgatttc
     1741 acgggccgcc gcacgcagcg cgcgcccgcg gatggcaacg gtacccatcg acgatgggaa
     1801 atcgcgatac ggaatctgac gatgattgaa acgataatcg gcgaaccggt agcagcctac
     1861 cgaatgacag caaaggcagc ggtcgcgacc cgcgcttggc gagttggcct acccgaacga
     1921 ttccgccacg aggcggatcc tgaacaccgg ctttttggcc tcgtccaaca gctccatctg
     1981 ccattcgccg ttttgtttca ggcttcggga aacgccacca acaaggttgc cgcaaacctt
     2041 ggtcatctcg gaccaggcat cttcacggct ctcgaattcg gacccgtggt cgggagcgcc
     2101 cgaatagcga ccatggctga cccggaaaaa atacagcgac atcattgaac ccatgcgttg
     2161 ggccgccccc aggcctgtcg aagatcggca ggttggatcg ggagtcgcta ccaacatatg
     2221 aatgtattca cccgtgtgac tacgggatga cgatgcgtca cgcgccagta atttcttgat
     2281 taaaacttgg ttgcgtgcgc cggtcagcac cggcgtcgtg tcagtcggtc gagcggcgaa
     2341 gctctagcac gttttccgat ttcgcaggct cggatttgac cggctcgatc gcgctgtgct
     2401 cgactggcgg tgtctcgaca cgcacggatc ccgtggtccg cgacgcatcg gccgagcggg
     2461 tctgctcctc cggcggatgc agcacacgcg gccgggccac cgcccgcgcc atcagcatct
     2521 gggcggcgcc ctgatgacgg aagtcgcaat aagcaaagcc catgcccgag accgaaccgc
     2581 ggaagttgcg ttcgtccttc ttgtcgagat tgaagcacgg ctcaaacgga aggcccttga
     2641 tcgaggcgca gacggactgg ccgcggacct gaagcgtatt gccgggaagg cgcatgtggc
     2701 gcaccggacc ggaaccgctg aattgtacgg cgcccatggc gctaccgtca tcgagcacac
     2761 ggcctgcgcc gcgggtgccg tcgaaacagg taaacgcgaa caccttgccg gagacgaact
     2821 tgcgggcttc ctcgccattc atgatctgtc cagcgagggc gggcgccatc accgcactgg
     2881 ccgtcaacgc acccaacaca aaacgcgcaa gcatgctaaa ctccaactta ccgagcaggg
     2941 gcaaccgctt gatatcttta cccgatgctt accataccaa ccgtggcaa
//
LOCUS       DASWGM010000328         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2527634,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000328 DASWGM010000000
VERSION     DASWGM010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527634"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VGA15_09435"
     CDS             <1..694
                     /locus_tag="VGA15_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9587953.1"
                     /translation="LAHALHHARHRSVFQKHLADQPLMQAVLSDMALHVEASTALVMR
                     LCRSFDHAPDDPGEAAFMRLLTPAIKYWVCKSAPGFLYEAMECLGGNGYVEEGILARH
                     FRESPVNAIWEGSGNVMCLDVLRALSREAEAASAVLQGLVEETTGLPGAADAAVFIGK
                     AFRRPDSERVARLAVEKLALLAAGAALNKVSPHQAELFAATRLAGNHASMYGAVDLAG
                     DDIRGLLERALP"
     gene            837..1622
                     /locus_tag="VGA15_09440"
     CDS             837..1622
                     /locus_tag="VGA15_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX9587954.1"
                     /translation="MDSLESTVLPAAPIAVIPAERQPRIWKFWGTALWGLFVFAAMFV
                     GQIAVVAWFVWRQEGPLDVAAAIHVLGGGLTISLSVIMGLPAVLAALWIAIRVSRTPF
                     ADYLALRWTSWTNFLIGTIALFVLVMGWDLLSRATGREVEPGFMGDVLKSASADGALW
                     LLVIAFCVAAPISEEFFARGFLYRGWSESFLGPAGAIVLSSVVWTGLHLQYDWFFLGE
                     VFSIGLLFGYLRYRSNSIWLTVVLHGLNNLAATLQTMWLAASS"
     gene            complement(1752..2243)
                     /gene="ruvX"
                     /locus_tag="VGA15_09445"
     CDS             complement(1752..2243)
                     /gene="ruvX"
                     /locus_tag="VGA15_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX9587955.1"
                     /translation="MPALILPLIEAAAHWPERGALVGLDLGTKTIGVAASDPDRRLAT
                     GVETIQRKGFKADAARLLAIAGERNAVGFVLGLPINMDASEGPRAQSTRAFARNFSKL
                     TDRAIALWDERLSTAAVERELIGMDMSRARRAEVIDEHAAIFILQGALDRLTKLHDMR
                     GER"
     gene            2300..2524
                     /locus_tag="VGA15_09450"
     CDS             2300..2524
                     /locus_tag="VGA15_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587956.1"
                     /translation="MDRLTLFGLFSVSAMLVCYALEERSHWFVLAFAAACALGSAYGF
                     LQGAWPFGLVEAIWAVVALRRWIVSQRRIA"
     gene            2613..>3301
                     /locus_tag="VGA15_09455"
     CDS             2613..>3301
                     /locus_tag="VGA15_09455"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9587957.1"
                     /translation="MWPDRRLLDLFKTEFPIVLAPMAGIMDADLVIAAAQGGALGSLP
                     CAMMSAEKAREQINIIRQRVSAPVNMNFFCHKAVDADSQREAGWKKRLGAYYEELGLD
                     PVAPVNAANRAPFDAAMCELVEELKPEVVSFHFGLPDQALLRRVKAAGCVVISSATIV
                     KEAIWLEENGADAVIAQGAEAGGHRGMFLTEDIAEQPGTFALVPQVVDAVKVPVIAAG
                     GIADGRGIAAA"
BASE COUNT          527 a          978 c         1121 g          675 t
ORIGIN      
        1 gctcgcgcat gcgttgcacc acgcccgcca tcgcagcgtg ttccagaaac atctcgccga
       61 tcagccgctg atgcaggcgg tgctttccga catggcgctg catgtcgagg cctcgaccgc
      121 gctggtgatg cggctctgcc gatcgttcga ccacgcgccg gacgatccgg gcgaagccgc
      181 cttcatgcgg ctgctgacgc cggccataaa atactgggtc tgcaagagcg cgccgggatt
      241 tctctacgag gcgatggagt gcctcggcgg caacggttat gtggaggagg gcattttggc
      301 acggcacttc cgcgagtcgc ctgtcaatgc gatctgggaa gggtctggaa acgtgatgtg
      361 cctcgacgtg ctgcgggcgc tgtcgcgcga ggcggaggcg gcatcggcgg ttttgcaggg
      421 ccttgtcgag gaaaccacgg gattgcccgg cgctgcggac gcggccgtgt tcatcggcaa
      481 ggcatttcgc aggccggact ctgagcgcgt ggcgcggctc gcagtcgaga agctggcgct
      541 gctcgcagca ggggccgctt tgaataaggt atcgccgcat caagcggaat tgttcgcagc
      601 aacgcgtctt gccgggaatc acgccagcat gtacggcgcc gtcgatctcg cgggcgacga
      661 tatcaggggt ttgctggagc gggcgctgcc ttgacgctcg accaatattt agttcgtcat
      721 ggccgggctt gtcccggcca tccacgtctt cgtcgccgag atagtaaggc gtggataccc
      781 ggcacaaggc cgggcatgac gaccacaaat cgctcacgtc aattgaaagc cgtttgatgg
      841 attcacttga aagtacagta cttccagccg cgcccattgc ggtcatcccc gccgagcgcc
      901 agccgagaat ctggaaattc tggggcaccg ccctgtgggg gctgttcgtc ttcgccgcga
      961 tgtttgtggg gcagatcgcg gtggtggcct ggtttgtgtg gcggcaggaa ggaccacttg
     1021 atgttgccgc ggccatccac gtcctcggcg gcgggctgac gatttcgctc tccgtgatca
     1081 tgggtttgcc tgctgttctc gcagcacttt ggatcgccat acgggtttcg cgcacgccct
     1141 tcgcggacta tctggcgctg cgctggacct cgtggaccaa ttttctgatc ggcaccatcg
     1201 cgctgtttgt tttggtgatg gggtgggacc tgctgtcgcg cgcgacgggg cgtgaggtcg
     1261 agcccggatt catgggcgac gtgctgaaat cggcgagcgc cgatggcgcg ctgtggcttc
     1321 tggtgattgc tttttgcgtc gcagccccga tctcagaaga gtttttcgcg cgcgggtttc
     1381 tctaccgcgg ctggtcggag tcgtttctcg ggcccgccgg cgccatcgtg ctgtcgtcgg
     1441 tggtgtggac ggggctgcat ctgcagtatg actggttctt tctcggcgaa gtgttttcga
     1501 tcggcctctt attcggctat ctccgctacc gcagcaattc gatctggctg acggtggtct
     1561 tgcatgggct caataatctg gctgcgacgc tgcagaccat gtggctcgcc gcatcaagct
     1621 gacggccgta atcagatcgc caccaacgcg atggcgatcg gcgtcgtcag cgcggatagg
     1681 tctgccagcg tgtcgcagcc tggaagatca aggtgaaggc cggccgccga ccgcgcattt
     1741 ggcggcgatc gctagcgttc tccacgcatg tcgtgcagct tcgtcaagcg atcgagggcg
     1801 ccctgcagga tgaagatcgc ggcgtgttcg tcgatcacct ccgcgcgccg ggcgcggctc
     1861 atatccatgc cgatcaattc gcgctcgacg gccgccgtgg agagccgctc gtcccacagc
     1921 gcgatggcgc gatcggtgag tttcgaaaaa ttgcgggcga atgcccgcgt cgattgcgcg
     1981 cgtgggcctt cgctcgcgtc catattgatg ggcaggccga gcacgaagcc caccgcgttg
     2041 cgttcaccgg caatggcgag caggcgcgcc gcatcggcct tgaaaccctt gcgctggatg
     2101 gtttcgacac cggtcgcgag cctgcgatcg ggatcggatg ccgcgacccc aatggtcttg
     2161 gtgcccaaat cgaggcccac aagtgcgccg cgttcaggcc agtgcgcggc cgcctcgatc
     2221 agcggtagaa tgagtgcagg catggcgttc gcataccacg cgggttgcaa tgggcgcaaa
     2281 gcgggaaagg ttaacgacaa tggaccggct gacgttgttt gggttgtttt cggtgtcagc
     2341 gatgctggtg tgttacgcgc tggaggagcg cagccattgg ttcgttctcg cgttcgcggc
     2401 ggcttgtgcg ctgggctcgg cctacggatt tctgcagggt gcctggccgt tcgggctggt
     2461 tgaggcgatc tgggcggttg tcgcgctgcg gcgctggatt gtcagccagc ggcgcattgc
     2521 atagccaggc cgccttgtga aatgctagtc ttgccaacgc cccggcaacg acgcccgcgc
     2581 cgaatcccct tttcgcctga atgagcacca ccatgtggcc ggaccgccga cttctcgacc
     2641 tcttcaagac tgaatttccc atcgttctcg cgccaatggc gggaatcatg gatgcggatc
     2701 tcgtgatcgc ggcggcgcaa ggcggagcgc tgggatcgct gccctgtgcc atgatgtcgg
     2761 cggaaaaggc acgggagcaa atcaacatca tcaggcagcg ggtgtcggcg ccggtcaaca
     2821 tgaacttttt ttgccacaag gcggtcgatg ccgattctca gcgcgaggcc ggatggaaaa
     2881 agcggcttgg ggcctactat gaagagttgg gtctcgaccc cgtggcgccg gtcaacgccg
     2941 ccaaccgcgc gccgttcgat gccgcgatgt gcgagctggt ggaagagctg aagccggaag
     3001 tggtcagctt ccatttcggc ctgccggatc aggcgttgct caggcgcgtc aaggccgcgg
     3061 gctgcgtcgt gatctcgtcc gcgaccattg ttaaggaggc gatctggctc gaggaaaacg
     3121 gcgccgacgc cgtcatcgcg caaggcgccg aagccggcgg ccatcgcggc atgttcctga
     3181 ccgaagacat cgccgagcag cccggcacct tcgcgctggt gccgcaggtg gttgacgccg
     3241 taaaggttcc agtgattgcg gcgggcggca tcgccgacgg gcgcggcatc gctgccgcgt
     3301 t
//
LOCUS       DASWGM010000329         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1545785,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000329 DASWGM010000000
VERSION     DASWGM010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545785"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VGA15_09460"
     CDS             complement(<1..1007)
                     /locus_tag="VGA15_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9587958.1"
                     /translation="MLGFAVVLAISAASMGIAYLGFERVSAGVASYRHSVSEADLARN
                     IDRELISYRALARYYVATGKEDDGKAALAAEASLKDAIDQSMKGTTNPAGLDQLTRLA
                     REFRAFTKIFAEILKVKAESALVTQNQLTRGGNMLRYKLDDLPSNAADTELQAIEFGV
                     KKVAAQFQTVTALASTFVINSDQTVAASALARIKFVENSLHAIPSTDEKIVQGLKEAS
                     TLLEEYRQALTKLIENSKSIDELTTEMTESAAAIMQGSGAMKAALLSDQQRLESESDA
                     TIGETERLIVMLAAGGFLLGGVLALMLGKGISRPMTAMCKAMRELAGGNFDVVLPGLG
                     R"
     gene            complement(1344..>1724)
                     /locus_tag="VGA15_09465"
     CDS             complement(1344..>1724)
                     /locus_tag="VGA15_09465"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9587959.1"
                     /translation="GAFEVSEKGDLANWTTEDPAFPPGIGGAMDLAVGAKEIRIIMEH
                     TSKAGEPRILMHCTYPLTAAGCVKRIYTNLAVIDVTPHGLVVIEMIPGMTVEKLQTLT
                     EPKLQLAPNWKALAPPEAPEACAA"
BASE COUNT          311 a          540 c          526 g          347 t
ORIGIN      
        1 ctgcggccga ggcccggcag cacgacatcg aaattgccgc cggcgagttc gcgcatggcc
       61 ttgcacatcg ccgtcatcgg gcgggaaatt cccttgccaa gcatcagcgc cagcacgccg
      121 ccgagcagga agccgccggc cgcgagcatc acgatcagcc gctcggtctc gccgatggtc
      181 gcgtccgatt ccgattcgag tctttgttga tcggaaagca gcgccgcttt catcgcgccc
      241 gagccctgca tgatggcggc cgccgactcg gtcatttcgg ttgtcagctc atcgatcgat
      301 ttcgaattct caatcagttt ggtcagggct tgccgatact cttcaagcaa tgtcgaggcc
      361 tccttgaggc cctgcacgat cttttcatcg gtcgacggta tcgcgtgaag cgagttttcg
      421 acaaacttga tgcgcgccag cgcgctggcg gccaccgtct ggtcggaatt gatgacgaag
      481 gtgctggcta gcgccgtcac tgtttgaaat tgcgccgcca cttttttcac gccgaattcg
      541 atcgcctgaa gctccgtgtc ggcggcgttg ctggggagat catcaagctt gtaacgcagc
      601 atgttgccgc cacgcgtgag ctggttctgc gtcaccagcg cgctctcggc cttgaccttg
      661 agaatctcgg caaaaatctt ggtgaaggcg cggaattcgc gggcgaggcg cgtgagctga
      721 tcgagccccg ccggattggt agtgcccttc atcgattgat cgatggcgtc cttgagactg
      781 gcttcggctg ccaacgccgc cttgccgtcg tcttcctttc cggtcgccac gtaatatcgc
      841 gccagcgccc ggtacgagat cagttcgcgg tcgatattgc gcgccagatc ggcctccgac
      901 acgctgtgtc ggtaggacgc cacgccggcg gagacgcgct caaatccaag ataggcgatg
      961 cccatgctgg cagccgagat cgccagcacg acggcaaagc caagcatgac tttcgcgcga
     1021 aagcgcaagg tcgggagttt tggcgatata gatcgcggcc agacacgtcc agacaatcgc
     1081 acgtcgaccc ccgtcttctt tcaacagcca gcagtacccg ggaacgtgag ttcccgggag
     1141 ccgatgccaa aaattagagc agaatggata aagggccgta aatttgcgcc gacttttccc
     1201 ccgtcatggc cgggcatagc cgtccgaagg acggcgtcgc ttccgctcgc ctatgcccgg
     1261 ccatccacga cttatcctcg ctgctagcct cttaagacgt ggatgcccgg gacaagcccg
     1321 ggcatgacga gcataaggag cgcctacgcc gcgcaggcct cgggggcctc cggcggcgcc
     1381 agggccttcc aattgggggc cagttgcagc ttcggctcgg tcagcgtttg cagcttctca
     1441 accgtcatgc ccggaatcat ttcgatcacg accaatccgt gcggggtcac atcgatcacc
     1501 gcgaggttgg tgtaaatgcg cttgacgcag ccggccgcgg tgagcggata ggtgcagtgc
     1561 atcaggatac gcggctcgcc cgccttgctg gtgtgctcca tgatgatgcg gatttccttg
     1621 gcgccgaccg cgagatccat ggcgccgccg atgccgggcg ggaatgccgg atcctcggtg
     1681 gtccagttcg caagatcgcc cttttcggag acctcgaagg cgcc
//
LOCUS       DASWGM010000330         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1831753,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000330 DASWGM010000000
VERSION     DASWGM010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831753"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGA15_09470"
     CDS             <1..693
                     /locus_tag="VGA15_09470"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964816.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9587960.1"
                     /translation="GAIHLGERDCSLQRRHQKVWEEGPSPALDAASRTKIGATCAKAM
                     QQMKYLGVGTVEFLYEDGEFYFIEMNTRIQVEHPVTEMITDIDLVLEQIRIAAGGDLP
                     VTQDEVIINGHSIECRVNAENPVTFRPSPGKILQYHPPGGLGVRIDSAVYQGYVIPPY
                     YDSLVGKLIVHGKTRAECLMRLRRALDEMVVEGIETTLPLFRALVREPAIIDGDYHIH
                     WLEQYLAGQPAA"
     gene            828..>2075
                     /locus_tag="VGA15_09475"
     CDS             828..>2075
                     /locus_tag="VGA15_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX9587961.1"
                     /translation="MMPDAPRWRATGQILLLAAGLVVLTVISAGSVYLVNKARDDARW
                     VVHTVEAENQISFTQLQLRRAESAQRGFLLTLQPNFQTDFEEAASQLAPALTRLGQLI
                     SDNPVERQLLDQMVALSNQRIEEFRQTIELARTQRLNDAAKIVREDVGREAMNRIYNL
                     AGGMRAEEDRLFVLRTTSADRSQTLAASMTGIGSGFVVVLAGISIFLVRRSARARDDA
                     EALLRDNNLNLETTIDERTTDLREANNEIQRFAYIVSHDLRSPLVNIMGFTSELEELR
                     GDIFRRIGTLGRAAASASPVAMIAGENEPVLGDEDKQLCQDFTEALGFIKSSIAKMDR
                     LISAILNLTREGRREFEPVRIETRELIEGIVATVAHQAAEAEAQIRIEPLPDIVSDRL
                     ALEQIFSNLIDNALKYLKPGVPGD"
BASE COUNT          410 a          666 c          623 g          376 t
ORIGIN      
        1 ggcgccatcc atctcggcga gcgcgactgc tcgttgcagc ggcgccacca aaaagtctgg
       61 gaagaaggcc cctcgccggc gctcgatgcg gcgtcgcgca caaaaatcgg cgcgacctgc
      121 gcgaaggcga tgcagcagat gaaatatctc ggcgtcggca ccgtggaatt tctctacgag
      181 gacggcgaat tctatttcat cgagatgaac acccgcatcc aggtcgaaca tcccgtcacc
      241 gagatgatca ccgatatcga cctcgtgctg gagcagatcc gcatcgcggc cggcggcgat
      301 ctgccggtga cgcaggacga agtcatcatc aacggccact ccatcgagtg ccgcgtcaac
      361 gccgaaaacc ccgtgacctt ccgcccctcg cccggcaaga tcctgcaata ccatccgccc
      421 ggcggcctcg gcgtgcggat cgattcggcg gtctaccagg gctacgtcat cccgccttat
      481 tacgactcgc tggtcggcaa gctgatcgtc catggcaaga cccgcgccga gtgcctgatg
      541 cggctgcgcc gcgcgctgga cgaaatggtg gtggaaggca tcgagaccac gctgccgctg
      601 ttccgggccc tggtccgcga acccgccatt atcgacggcg actaccacat ccactggctt
      661 gaacagtatc tagccggcca gccggccgct tgagccgctg tttagcccac atcggttgca
      721 ggccccctgg aaccaattgg ccgaaccggc gttctggttg gctgggaaac atctttcctg
      781 ttcgggggga ctgcttgaac ttcgttcgct tatgacgcga gacggtcgtg atgcctgatg
      841 ccccgcgctg gcgtgcgacg ggccaaatcc tgctgcttgc ggcgggcctt gtggtgctga
      901 cggttatcag cgccggttcg gtctatctgg tgaacaaggc gcgggatgac gcgcgctggg
      961 ttgtgcacac cgttgaagcc gaaaatcaga tatccttcac gcaattgcaa ttgcgccggg
     1021 ccgaaagcgc gcagcgcggt ttccttctga cattgcagcc caattttcaa accgatttcg
     1081 aggaagctgc atcccagcta gcgccggcat taacgcggct gggccaactc ataagcgaca
     1141 atcccgttga aaggcagcta ctggaccaga tggttgccct cagcaatcag cggatcgagg
     1201 aattccgtca aaccatcgaa cttgcccgga cccagcgctt gaatgatgcc gcgaagatcg
     1261 tgcgcgagga cgtcggccgc gaggcgatga accgcatcta caatctggcc ggtgggatgc
     1321 gcgcggaaga agatcgtctg ttcgtgctgc gcacgacgag tgcagaccgc agtcagaccc
     1381 tggccgcgtc gatgaccggc atcggttcgg gtttcgttgt cgtgctggcc ggcatctcga
     1441 tctttctggt acggcgctcc gcgcgcgccc gcgacgacgc cgaagccctg ttgcgcgaca
     1501 acaacctcaa tctcgaaacc acgatcgacg agcgcaccac cgatcttcgc gaggccaaca
     1561 acgagatcca gcgcttcgcc tatatcgtca gccacgatct gcgctcgccg ctggtgaaca
     1621 tcatgggctt taccagcgaa ctcgaggaac tgcgcggcga cattttcagg cggattggga
     1681 cactcggccg tgccgcggct tcggcttcgc cggtggcaat gatcgccggc gagaacgagc
     1741 cggtccttgg cgacgaagac aagcagcttt gccaggattt caccgaagcg ctgggattca
     1801 tcaagtcgtc gatcgccaag atggaccgcc tgatatcggc gatcctcaac ctcacccgcg
     1861 aggggcggcg ggaattcgag ccggtgcgga tcgagacccg cgaactgatc gaaggcatcg
     1921 tggccaccgt cgctcaccag gccgccgagg ccgaagccca gattcgcatc gagccgctgc
     1981 cggacatcgt cagcgaccgc ctcgcgctgg aacagatttt ctccaatctg atcgacaacg
     2041 cgctcaaata tctcaagccg ggagttcccg gcgac
//
LOCUS       DASWGM010000331         8379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2527991,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000331 DASWGM010000000
VERSION     DASWGM010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2527991"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VGA15_09480"
     CDS             complement(<1..593)
                     /locus_tag="VGA15_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587962.1"
                     /translation="MDAVMLPSSTAGAMAQAPSPQAIAEYRRKLKEYLEARAAFDQEA
                     GAYWNSIADKRRVRNAKRRDRQSIALDDYVLTQPPVYSGPKRPVDPSPAPPEPEEPRR
                     ERKYIPVVADLLKAAAEQFQFTPQRPANEVDFKRAYARVAAAAGLTREQAVRVYSFET
                     GGNGNHDMQSGLSASRPGSRAISTAIGYNQLLTTNTV"
     gene            complement(743..1375)
                     /locus_tag="VGA15_09485"
     CDS             complement(743..1375)
                     /locus_tag="VGA15_09485"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9587963.1"
                     /translation="MTRPFDDTTRRNIAERCAAFARLPASEPAPALKRAAVAIALTEA
                     ADAADGTAVLLTLRTAGLRAHRGQWALPGGRCDEGETAVTAALRELHEELGLELGEDN
                     VLGWLDEYPTRSGYLITPVVVWAAAGAVISPNPEEVASVHRIALFDIERTDAFDFVAI
                     AESTRSVIRFRHAGQFIHAPTAALIYQFREVLAGRDTRVAELEQPVFAWK"
     gene            1456..1881
                     /locus_tag="VGA15_09490"
     CDS             1456..1881
                     /locus_tag="VGA15_09490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9587964.1"
                     /translation="MTDKAAEQLKSSGWTIVDTTGFLHLVGPLWQRVVDNEHEYALVA
                     QDKHHNRRGLVQGGLLMTFADRSCGMTARYVSGRPTLATIQLDTHFVDSGKIGDILIS
                     RPRVVRSTRSLIFMTTEVTADKRCIVMASGVFKILKNDT"
     gene            2042..3055
                     /locus_tag="VGA15_09495"
     CDS             2042..3055
                     /locus_tag="VGA15_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9587965.1"
                     /translation="MQYRQLGRSGLKISPICLGTMMFGGPTDEATSTRIVAKAREAGV
                     NFIDTADAYSSGNSEQVVGRAISNNRQNWVLATKLANPMGDDPNRGGLSRRWVLQAAD
                     ESLKRLGTDYIDIYYLHKEDHSTPLHETVRAMGELIQNGKVRYFGVSNYRAWRVAEIC
                     NICDNIGIDRPIASQPYYNAMNRMPEVEHFPACGYYGLGIVPYSPLARGVLTGKYSPD
                     APPDKDSRAGRNDTRMMQTEWRPESLQLARQIRQHAEAKGITAGQFAVSWVLNSSFVS
                     AVIAGPRTEEQWDDYLRALDYRFTAEDEALIDRLVTSGHPSTPGYNDPAYPIEGRRAR
                     TSI"
     gene            3246..3509
                     /locus_tag="VGA15_09500"
     CDS             3246..3509
                     /locus_tag="VGA15_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587966.1"
                     /translation="MTRSRRETVTFRHPFRIKGIDRQLQAGAYEVVTDEELIEGLSFP
                     CFRRVATLIMVPGAPPHHQSTEMLAISSVDLADAQQADAVASL"
     gene            3580..3759
                     /locus_tag="VGA15_09505"
     CDS             3580..3759
                     /locus_tag="VGA15_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587967.1"
                     /translation="MQILSARHVKTEESQRLGVVSGWYSTKVSGTFVTGPHTTQEDCL
                     TKIAELNPAPAKKKF"
     gene            3793..4077
                     /locus_tag="VGA15_09510"
     CDS             3793..4077
                     /locus_tag="VGA15_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 family protein"
                     /protein_id="HEX9587968.1"
                     /translation="MALTQGSFQEYDFNRMVVLFTMKNDEANVACAISTDAMDQLEGS
                     SRTPPGQREQQFLRLRDRIEARAARKFSDVELEGNPPGVILRAIDFRAPR"
     gene            complement(4635..5261)
                     /locus_tag="VGA15_09515"
     CDS             complement(4635..5261)
                     /locus_tag="VGA15_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587969.1"
                     /translation="MDILVILGGLLLALVVAGFSSARSYFEKGRLQGMEEATRELSRG
                     ISSHYELEGQAVPESVAKALKGFNAVARKRWKKRKGSTDPYHAQLWTVGDAIGEACWT
                     KGHSAGIRRRAPAEGKIRVDLSLTELLQLSWLAHLGFQHMMPNHRGFEIHRFSGEEDA
                     LEGAKAVGKIESVIPAKERPFADLAAQLKSRQKLIFDWWQPVPDRLTA"
     gene            complement(5500..5727)
                     /locus_tag="VGA15_09520"
     CDS             complement(5500..5727)
                     /locus_tag="VGA15_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587970.1"
                     /translation="MKKSVYVSLVLLLAAGSSATAQSGPTQEEKMAWRSDATAFCAEH
                     IGKPPQMNACLRENKAKLSVSCRKVVEARGG"
     gene            6004..6183
                     /locus_tag="VGA15_09525"
     CDS             6004..6183
                     /locus_tag="VGA15_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587971.1"
                     /translation="MLKKKRWVSAALALTALSTLASVSLGAGSAEAAVVYCKTAGVPQ
                     GCVVRPVVPAACPED"
     gene            6268..6588
                     /locus_tag="VGA15_09530"
     CDS             6268..6588
                     /locus_tag="VGA15_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587972.1"
                     /translation="MKRSKQNAALSRGVAGSGRLWAPRLAQESRLRQLEDSFARHRVE
                     GEAVEPEIERAVLLGALNRRADAQQAFIDILRRAPANFSALNEFGALLTDMGAIDAAC
                     RVPL"
     gene            6659..7357
                     /locus_tag="VGA15_09535"
     CDS             6659..7357
                     /locus_tag="VGA15_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587973.1"
                     /translation="MMKTGRISNARRLGAVAGVVTPRTIAMPRDLLAGADGASSIADR
                     QFTFPLLLRSPGYHTGRNFILVASAAELAAAAVSLPGADLLVIEYLDARGKDGNARKY
                     RVMMIGGQIYPLHLAISRQWKVHYFTSDMADKPDHRLEEASFLGDMPAALGDKAIAAL
                     EQIRDALGLDYAGIDFGLGPDGDLLLFEANATMVIVPPNPDERWAYRRAAIGKILDAV
                     VAMIVQKSAQAGRA"
BASE COUNT         1625 a         2653 c         2555 g         1546 t
ORIGIN      
        1 tcgacggtgt tggtggtgag cagctgattg tagccgatgg cggtcgagat cgcgcgcgag
       61 cccggccgcg aggcgctcaa tcccgattgc atgtcgtgat tgccgttgcc gccggtctcg
      121 aacgaataga cccgcaccgc ctgctcgcgc gtgagcccgg ccgcggcggc cacgcgggca
      181 taggcccgct tgaaatcgac ttcgttggca ggccgctgcg gagtaaactg gaactgctcc
      241 gcagccgcct tcaacagatc ggcgacgact ggaatatatt tgcgctcgcg gcgcggttcc
      301 tcgggctcgg gcggcgctgg cgacgggtcc accggccgct tcggcccgga atacaccggc
      361 ggttgcgtca gcacataatc atcgagcgcg atcgattggc gatcgcgccg cttggcgttg
      421 cggacccgcc gcttgtcggc gatcgaattc cagtaagcgc cggcttcctg gtcgaacgcc
      481 gcgcgcgctt cgagatactc cttcagcttg cggcgatatt ccgcaatcgc ctggggcgaa
      541 ggcgcctgcg ccatcgcgcc cgccgtcgat gacgggagca taacggcatc catcgcgacc
      601 gcctgctgag cgatcagcgg cagcgcgagt gcaaaaaacc tgcggcaaaa gcgaatcgca
      661 tcggggcgca tcgggttgtt gtagagcaag cgtggcgcgc aagtcagccc ccgaaacggc
      721 gcgattgcgg cgtggcaggc gcctacttcc aggcaaatac cggctgttcc agctcggcaa
      781 cgcgggtatc gcggccggcc agcacttcgc ggaactgata gatcaaggcc gccgtcggcg
      841 cgtggatgaa ctggccggcg tgacgaaacc ggatcacgct ccgcgtgctt tcggcaattg
      901 cgacgaagtc gaaggcgtcc gtccgctcga tatcaaaaag cgcgatgcga tgcaccgagg
      961 cgacctcttc cggattgggc gaaattacgg cgcccgcggc cgcccacacc acgaccggcg
     1021 tgatgagata gccggagcgc gtgggatatt cgtcgagcca acccagcaca ttgtcttcgc
     1081 cgagctcaag gccgagctct tcgtgaagtt cgcgcagcgc cgccgtgacc gctgtctcac
     1141 cctcgtcgca gcgtccgccc ggcagcgccc attgaccgcg atgggcgcgc aagcctgccg
     1201 tgcgcagggt caacagcaca gccgttccgt ctgccgcatc ggcggcttcg gtgagcgcga
     1261 tggcgacggc ggcccgcttc agtgccggcg ccggttcact tgccggcagc cgtgcaaacg
     1321 ccgcgcaacg ctctgcgata ttccgccggg tggtatcatc aaatggccgg gtcatcccgc
     1381 ttgactacaa cacgtcggcc attgatgaaa tgaccacaag acctgaattc gccgccgaga
     1441 ctggaaacac ccgacatgac cgacaaggcc gcggagcagc tcaaatcctc cggctggacc
     1501 atcgtcgaca caactgggtt cctgcatctg gtcgggcctt tgtggcagcg ggttgtcgat
     1561 aacgagcacg aatatgcgct cgtcgcgcag gacaagcacc acaaccgccg cggcctggtc
     1621 cagggcgggc tgttgatgac attcgcggat cgcagctgcg gcatgacggc acgatacgta
     1681 tcgggccggc caacactggc gacgatccag ctcgacaccc attttgtcga cagcggcaag
     1741 atcggcgata tcctgatctc caggccgcgc gtggtccgct cgacccgcag cctgattttc
     1801 atgaccaccg aggtaaccgc cgacaagcgt tgtattgtga tggccagcgg cgtgtttaag
     1861 atattgaaga acgatacgta gcgcttgcgg ccatcgcgag gggccacgaa acacactccg
     1921 tcattccggg atggtccgaa ggaccaggcc cggaatctcg agattccggg ttcgcgctgc
     1981 gcgcgccccg gaatgacggc gcataaccgg aatgacggcg cataccaaga ggaccaaccc
     2041 catgcaatat cgtcaactcg gccgcagcgg cctgaaaatc tcgccgatct gtctcggcac
     2101 catgatgttc ggcgggccga ccgatgaagc gacctcgacg cggatcgttg cgaaggcgcg
     2161 cgaggccggc gtcaatttca tcgataccgc ggacgcctat tcaagcggca attccgagca
     2221 ggtggtcggc cgcgccattt ccaacaaccg ccagaactgg gtgctcgcga ccaagcttgc
     2281 caaccccatg ggcgacgatc ccaatcgcgg cgggctgtcg cggcgctggg tattgcaggc
     2341 cgccgacgaa agcctgaaac ggctcggcac cgactacatc gacatctatt atttgcacaa
     2401 ggaagatcat tcgacgccgc tgcacgagac cgtgcgcgcg atgggcgaac tgatccagaa
     2461 cggcaaggtg cgttacttcg gcgtctcgaa ctatcgggcc tggcgggtcg ccgaaatctg
     2521 caacatctgc gataacatcg gcattgaccg cccgatcgcc agccagccct attacaacgc
     2581 gatgaaccgc atgcccgaag tggagcactt tccggcctgc ggatattacg gcctcggcat
     2641 cgtgccctac agccccttgg cgcgcggcgt gctgaccgga aaatacagcc ccgatgcacc
     2701 gcccgacaag gacagccgcg ccggccgcaa cgacacccgc atgatgcaga ccgagtggcg
     2761 gccggaatcg ctgcaactgg cgcggcaaat caggcagcat gccgaggcga agggaatcac
     2821 cgccgggcag ttcgcggtgt cgtgggtgct gaattcttcg ttcgtctcgg ccgtgatcgc
     2881 aggcccgcgc acggaagagc aatgggatga ttatttgcgc gcgctcgact atcgcttcac
     2941 cgccgaggac gaagccctga tcgatcgcct cgtcacatcg ggccatccct ccacgccggg
     3001 gtataacgat ccggcgtatc cgatcgaagg gcgaagggca cgcacctcga tctaaaaaag
     3061 aaagtcgtgg ctatttgggg ctaaaaggcg cacacgtccg gtgttcgcct cacgcgccgg
     3121 gtgcacggct gtcttttcgc ggggctgcga acagacgatt tgcaacattt gctggcgaaa
     3181 cagcgcgaaa gcggttcaat tttcattgaa tcggctgggc ccgcgccaat tttggggtgg
     3241 tgagcttgac gaggtcgcgg cgcgaaacgg tcactttcag gcatccgttc cggatcaagg
     3301 gcatcgatcg ccagctgcag gcgggagcct atgaagtcgt caccgacgaa gagctgatcg
     3361 aaggcctgtc gtttccctgc ttccgccgcg tcgccaccct gatcatggtt cccggcgcac
     3421 cgccgcacca tcagtcgacc gagatgcttg ccatcagttc ggtcgatctc gccgatgcgc
     3481 agcaggccga tgcggtggca tcgctatgaa gacgcatccc gcgccaatcg aaaccactcg
     3541 ctgcgtccag aaaataacat cgcacctcaa ggaatcccca tgcaaattct ctccgcgcgt
     3601 cacgtcaaaa ccgaagagtc acagcgtctc ggcgtcgtct ccggctggta cagcaccaag
     3661 gtgagcggca ccttcgtcac cggtccccat accacgcagg aagattgcct gacgaaaatc
     3721 gccgagctca atccggcacc agccaagaag aaattctgaa ctgacagtct gttcaatcga
     3781 ggagacttga agatggcgct cacccaaggc agctttcagg agtacgactt caaccggatg
     3841 gtcgtgctgt ttacgatgaa gaatgacgag gccaatgtcg cctgcgccat ttccaccgac
     3901 gcgatggatc agctcgaggg ctcgtcgcga acaccgccgg gccagcgcga gcagcaattc
     3961 ctgcgcctgc gcgaccggat cgaggcgcgc gcggcgcgga aattcagcga tgtcgaactg
     4021 gaaggcaacc ctccgggcgt gatcctgcgc gcgatcgatt tccgcgcgcc gcgctgaatg
     4081 atcgggcaac gcgaggccgg gcaggatccc accgccctca cttacggaag atgccgcccg
     4141 acatccgaaa actaattagc gcttcttctc cggcgctccg gaatcccgat gcaggtggtc
     4201 gtaccgtcgg tttccctggc ggccgtgctg caaggcacag cctttactat cttggtggag
     4261 acaccgtcac tggcgacgat gacccggccg gtttggctat tcggatccag cgggccgcct
     4321 acctccggaa ccacgttcgg gttcacgggt gttcccattg cgggcggcgc caatccgaga
     4381 ttcgagggcg gcggactggc tgtaaccgtc cccgttgtct gttgggcaag tcccgccaac
     4441 ggtagcatgg taaccgcgac ggcagcgata agggtgattc tgcgcattga agcctcccta
     4501 tggggtgcat caatagaatg gaatgttccc gcaaaagttc aacctcggcc aaggattttg
     4561 aaggattttg ccgggcgcat cgcgatgaga tcggggagcg gcttttccgc gatgccgcga
     4621 atgcttcgct gctatcaagc cgtcagccga tccggcaccg gctgccacca atcgaagatc
     4681 agcttctgcc ggctcttgag ctgagccgcg agatcggcga acggccgttc cttcgcagga
     4741 atgacgctct cgatctttcc gacggctttg gcgccttcca gcgcatcttc ctcgccgctg
     4801 aagcggtgaa tttcgaagcc acgatggttc ggcatcatgt gttgaaagcc gagatgcgcc
     4861 aaccagctga gttgcaatag ttcggtgagc gagagatcga cgcggatctt tccctccgcg
     4921 ggcgcccttc ttctgatgcc ggcggaatgt cccttcgtcc agcaggcctc gccgatcgcg
     4981 tcgccaaccg tccacagttg cgcgtgatac ggatcggtcg agcccttgcg ctttttccaa
     5041 cgtttccgag caacggcgtt gaaacccttg agggcttttg cgacgctctc gggaacagcc
     5101 tgaccttcga gttcgtagtg cgagctgatc ccccgggaga gttcgcgggt ggcttcctcc
     5161 atgccctgga gccgcccctt ctcaaaatac gatcttgcgc tggagaagcc ggcgacgaca
     5221 agcgccaaca gcaagccacc cagaatgacc agaatatcca cggccccaaa ccccactttg
     5281 gcccgaagca cattcgagcc gatcgcccca cgtcaaattt aaaccaactt cgttaatgca
     5341 accgtaaggg gcggtggcgc agccgcgccg gcgttgccga tcgttccgtt ggcccgccgc
     5401 tcacgcggcg gatcggacct ttggcggagg aagcctcgtc gcgacccgct acagccgcgc
     5461 cgcttcgatg gccgcatccg ggacgcggcg gataaacgct cacccgccgc gggcttccac
     5521 aaccttgcgg caggagacgg agagtttggc cttgttctcg cgcagacatg cgttcatctg
     5581 cggcggcttg ccgatatgct cggcgcagaa cgcggtcgca tcggaacgcc aggccatctt
     5641 ctcttcctga gtaggtccgg attgcgcggt cgccgacgag ccggcagcca gcagcaggac
     5701 aagcgagaca tagactgact tcttcatgga tcacctttcg acacgttgat ttgtggggtt
     5761 tgaagttaag catccggaac atcgtcgagg tgaagatcac ggtcgatgcc atgttcatgg
     5821 caccgcatga tcggttcact gtcgcatctc tcacgcatga ttcgatccaa actccgcgcg
     5881 gcggccgaca cccccatcta catgggatgt tacggcgcgc ggccatgtcc catgcacctc
     5941 tcgcgtcggc tgcggagacc gatcggtcca tcgcaaggcc gcacgtttaa caggagagat
     6001 gacatgttga aaaaaaagcg atgggtgagc gccgctctgg cattgacggc actttcgacc
     6061 ctggcgtcgg tttcactggg cgccggctca gccgaagccg ccgtggtcta ttgcaagacg
     6121 gccggcgtgc cgcagggctg cgttgttcgg ccggtcgtac cggcggcctg tcccgaggac
     6181 tgacgatcaa gctgtcggat gcgcgggtgc gaatcagcag gcaagtggta tgattgcaaa
     6241 tctccgacgt cgagccttgc acgcaccgtg aaaagatcga agcagaacgc ggcactctcg
     6301 cgcggcgttg ccggcagcgg tcgcctgtgg gcgccccgat tggcgcagga atcgagactg
     6361 agacagcttg aggattcttt cgcgcgtcac cgcgtcgagg gcgaggccgt tgagcccgag
     6421 atcgagcgcg cggttctcct tggcgccctc aatcggcgag cggatgccca gcaggccttc
     6481 atcgatatcc tgcgaagagc gcccgcgaat ttcagtgccc tcaacgaatt cggcgccctg
     6541 ctgacggata tgggcgcgat cgacgcggcc tgccgggtcc ctctgtgaac ccgctctgga
     6601 ggcggccgct ggcctgatcg ctcggaccag cgcaccggtt atcaacgatc cccgcgcggt
     6661 gatgaaaacc ggccgtatca gcaacgcccg gcggctcggc gccgtagcgg gggtggtgac
     6721 gccgcgaacc atcgcaatgc cgcgcgacct cctcgccggg gcggatggcg ccagttcgat
     6781 cgcggaccgt cagtttacgt ttccgctgct gttgcgttcc cccggctatc ataccgggcg
     6841 caacttcatc ctcgttgcct cggcagccga gttagccgcg gcggcggtga gcctgccggg
     6901 agccgatctg ctggtgatcg agtatctcga cgcccgcggc aaggatggca acgcgcgaaa
     6961 ataccgcgtg atgatgatcg gaggtcagat ctatccactg catctcgcga tatcccggca
     7021 atggaaagtg cattatttca cttccgatat ggccgacaag ccggaccatc ggctggagga
     7081 agcgagcttt ctcggcgata tgccggccgc cctcggagac aaggcgatcg cggcgcttga
     7141 acagatccgg gacgccctcg gcctcgacta tgctggaatc gatttcgggc tcggtcccga
     7201 cggcgatcta ttgctgttcg aggccaatgc aaccatggtg atcgtcccgc ctaaccctga
     7261 cgagcgctgg gcctatcgcc gcgctgccat cggcaaaata cttgacgccg tcgtcgccat
     7321 gatcgtgcaa aaatcggcgc aggcggggcg agcctaggtc tccctggcaa ctgatggctc
     7381 gacgggtgcc ctgggggcgg gatcgaatcg cgacaccgcg gcgttcgacc gcatgatcaa
     7441 cggcattttg ccgcggttgc tggatattgg cccctcacct ctccaaaact tgatcgtcgc
     7501 aatcaaggct cggaattccc gaacgcctta ttagattcaa tttaatggcg tcgacggatt
     7561 ggggaaccta catcaactgc gcacgaatca cccttatgct gaaatcatca tggagctttc
     7621 ccgatgtcga acacctcggt aaaagtcgct ctcgcattaa tcgtggtctt ggccctccct
     7681 gccgcattat ttgcccaaca cgccggctcg gcggcaacgg gaaacgttcc catcacgaca
     7741 atcgatccca gcggggcggg gaaatgcttc caaggtggcc ccgctaccgc ccccgcgcat
     7801 aaccgtcacc gtcccgaaat tgttcattcc aacggcgttt gctgggcccc gcggaatacc
     7861 gctaacggtc cagaagttta agggctgcga agatgagaaa acccgccgcg cgagcgggcg
     7921 agttcaccgt tcctcgtccg gatgcatctg gtcgcatatg ggcgccctac cctttgaatg
     7981 atgatgcgag gcctagcgtg cgaatgtagc cgacgctgtc agggcaacga tcagggtcgc
     8041 cataagcgtt tgcccactcg gcccctcact tcatccgcgg gtttttccgc tcggccgtcc
     8101 gacgcgataa gaccaatagc cagagccccg tttcgatgga atcgaaacgg ggctctggat
     8161 tcttgatttg tcgcgttttc ttgacgcgaa ccgatctcca cccccggatc aagtccgagg
     8221 gcatgcttcg cgggaaaacg ctctagtact actgcgtcac aagttcatcc gcacgcgtcg
     8281 cgcggttgaa gcgcgacagc atgggcatcg tggcaagcgc ctgacgagag cgtcgatgat
     8341 gcgtcggcgc acggtggcga tcgagtttgg gacgtgacg
//
LOCUS       DASWGM010000332         6890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2528031,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000332 DASWGM010000000
VERSION     DASWGM010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528031"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VGA15_09540"
     CDS             complement(<1..776)
                     /locus_tag="VGA15_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9587974.1"
                     /translation="MTFATVMVSLALDQSNAARLEAAGQMAERFDAGIVGIAAAQFSP
                     PLYFMSGEQAQNLIDQGEASIKKRMSELELQFREATKNRARHVEWRSAIDFPARYIVQ
                     QARCADIVVSGGQGKNFSDPFALASPKDLVMQAGRPLFIAPDNVSWLDLRSVLVAWKD
                     TPEARRAIADSLPMLRKAKDVTVAEVVEAGDSRPAAVSRVRDVVAWLSRHGVSASELV
                     PERSGDAIAQLEAIAANVGAGVVVAGAYGHSRFRELVLGG"
     gene            1073..2593
                     /gene="fixL"
                     /locus_tag="VGA15_09545"
     CDS             1073..2593
                     /gene="fixL"
                     /locus_tag="VGA15_09545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein FixL"
                     /protein_id="HEX9587975.1"
                     /translation="MPGTIRSSPDDPVRNEHLRLRIEGFGVGTWDLDLSTRALEWSDT
                     TRNLFGVALDRPVTYELFLSLLEPRDRERTEQAIKRTAEVGGNFDVAFRVGRTPGSGQ
                     WMRLRGGLVKDDAGVSRHLSGIVLDIDEEKQLEEALRTRESHLRSILDTVPDAMIVID
                     GQGIVQFFSTAAERLFGYTEQEAIGKNVSALMPNPDRERHDGYLARYRSSGERHIIGI
                     GRIVTGQRRDRTIFPMHLTIGEMQSGGEPYFTGFVRDLTEHQQTQARLQELQSELVHV
                     SRLSAMGEMASALAHELNQPLAAISNYMKGCRRLLAGSTDPNTQKIENAMDRAAEQAL
                     RAGQIIRRLRDFVSRGESEKRVESLAKLIEEAGALGLAGAREQNIQLRFNLNPEVDLV
                     LVDRVQIQQVLVNLFRNALEAMAQSRQRELSASNAKVADDMIEIAVSDTGTGFNDDVK
                     PNLFQTFFTTKETGMGVGLSISRSIIEAHGGRMWAENNAAGGATFRFTLPAAPGES"
     gene            2597..3214
                     /gene="fixJ"
                     /locus_tag="VGA15_09550"
     CDS             2597..3214
                     /gene="fixJ"
                     /locus_tag="VGA15_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HEX9587976.1"
                     /translation="MSSKTKVYVIDDDEAMRDSLDFLLVSADFNVTLFETALDFLDAL
                     PVLEFGCVVSDVRMPGLDGIELLKRMKAANSPFPIVIMTGHGDVPLAVEAMKLGAADF
                     LEKPFEDDRLIGMIEAAIRQAEPAAKSEVVTLNIAARIATLSPRERQVMEGLIAGLSN
                     KLIARDYDISPRTIEVYRANVMTKMQANNLSELVRLAMRAGMLND"
     gene            3300..3692
                     /locus_tag="VGA15_09555"
     CDS             3300..3692
                     /locus_tag="VGA15_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9587977.1"
                     /translation="MASLAKPTVYVVDDDADVLGSLRFLLEADGFAVRTFRNGPALLN
                     AVRLTGVDCFVIDYKMPDINGIDLASRLRNRDITAPIILITGYPDENISARASAAGIR
                     HVVQKPLLEESLLTHIRGAIQGDHPVTG"
     gene            3812..4507
                     /locus_tag="VGA15_09560"
     CDS             3812..4507
                     /locus_tag="VGA15_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9587978.1"
                     /translation="MLTQPLSTSVIGSKIGRVACPTPASDQFGAITGHAGLVATEFTY
                     KKDEEIYGEDEPSEYVYQVISGAVRSYKLLSDGRRQIGTFHLPGDVFGLESGAVHRLA
                     AEAIIDTTVRLVKRRSLEQAAGVDVNVARSLWTMTAGELRHAEDHMLLLGRKTAMERV
                     ATFLLEMDRRLAVAGMMALPMCRRDIGDYLGLTLETVSRALSQLHAQGVLGFSGARQI
                     VLRNRQRLRNMDA"
     gene            complement(4517..5695)
                     /locus_tag="VGA15_09565"
     CDS             complement(4517..5695)
                     /locus_tag="VGA15_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9587979.1"
                     /translation="MRGLDWFIFFLADVQTGFGPFIAVYLTTQKWTQVEIGLVLSIGG
                     LVGLIGQMPGGAIVDAARSERLVAALAVATIGVSALGYAAWPIFPVVVVAATLHAAAS
                     CVLGPAIAAISLGLVGPLAIGERFGRNARYASLGNGVAAAVMGTCGYLLSSRSVFLVT
                     FILAIPTLLALARIREWEIDAAQAHGAVPREVPDANATSVLNLMCRRPLLIFAGSVFL
                     LQLANAAMLPLMAGVVTTRSSQWAPVLIAACIVVPQAIVALTSPSVGRKAQQWGRRPL
                     LLLGFATLAIRGVLFATVSDPYLLVAVQVFDGITAAVFSVMVPLIIADVAFGSGHFNL
                     AQGIVGTATGIGASLSTVLAGYVSDRFGSSIAFTGLAAVAATGLVLIWLVMPETRRTT
                     "
     gene            complement(5852..6361)
                     /locus_tag="VGA15_09570"
     CDS             complement(5852..6361)
                     /locus_tag="VGA15_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX9587980.1"
                     /translation="MRAKRLSMFIAIAVVASTAAISWAADDKGLPQAGRDAPPPTTAP
                     AVPSKEAVPPPSVTIIRAADAHGVLGREVRSAADENMGRIVDIIVDRSGSVRAAVIDF
                     GGFLGVGSRKIVVDWNALHFGRIANKSDSITLELTKDQVTAAPEYKEDTPIVVLGASG
                     SLQPLQFGP"
     gene            complement(6542..>6890)
                     /locus_tag="VGA15_09575"
     CDS             complement(6542..>6890)
                     /locus_tag="VGA15_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX9587981.1"
                     /translation="SYMDLRRLPELFCGFQRERRRGPTLYPVACAPQAWASATPFTLL
                     EAALGLEFDAQRGEIRLRNPRLPAFLNEVFLRDLQLGPSSVDLRVRRHGDDVSLEVLR
                     TRGQIQVSIVLAH"
BASE COUNT         1408 a         2149 c         2114 g         1219 t
ORIGIN      
        1 atgccgccga gaaccaattc gcggaatcgc gaatgaccgt aggcgccggc gaccacgaca
       61 cccgccccga cattggcggc tatggcctcc agctgagcta tcgcgtcccc gctcctctcc
      121 ggtacgagct cggaggcgga aacgccatga cgtgacagcc acgccaccac atccctgacc
      181 cgcgacacgg cggccggccg actgtcgccg gcttccacga cctcggcgac ggtgacgtcc
      241 ttggccttgc ggagcatcgg cagggaatcg gcgatcgccc gccgcgcctc cggcgtgtcc
      301 ttccacgcca ccagaacgct tcgcaagtcg agccaactga cattatccgg tgcaatgaac
      361 agcggccggc cggcctgcat caccagatcc ttcgggctgg ccagcgcgaa tgggtccgaa
      421 aaatttttac cctgtccgcc gctcacgacg atgtcggcgc aacgcgcttg ttgcacgatg
      481 taccgtgccg ggaaatcgat ggcgctgcgc cactcgacat gcctggcgcg atttttggtc
      541 gcctcgcgaa attgcagttc cagttccgac atgcgtttct tgatcgatgc ctcgccctgg
      601 tcgatcagat tttgcgcctg ctcccccgac atgaaataaa gcggcggact gaactgcgcc
      661 gccgcgatcc cgacgatccc ggcatcgaat cgctcggcca tctggccggc ggcttcgaga
      721 cgcgccgcgt tcgactgatc cagcgccagg ctgaccatga cggtcgcaaa tgtcatccga
      781 tctctccgtt gtcgcagccg acaactcctt ctaggcccga atcccggggc caggatgaga
      841 tagatcaaag tccccaaacc gagggttgag ccgaacatcg agggatattc gggttggata
      901 tcgatccgcc ggaacttgcg tccacgtcgc cgtcaggcga aattgccggc tcggcagcgc
      961 cgtttcaggg ctatggcagc ttcctgacgt caatctcgcg taggttgttc gacttggagc
     1021 tcgccggccc agccggcggc ggctgagaca gatggaagtg aaggcttaac gcgtgcccgg
     1081 cacaattcga tcatcgcctg acgaccccgt acgcaacgag catctaaggc tgcgaatcga
     1141 gggatttggc gtcgggacct gggatctcga cctttcaacg cgagcgttgg agtggtccga
     1201 taccacgaga aaccttttcg gcgtcgccct cgaccggccg gttacgtacg agctgtttct
     1261 ttctctgctc gaaccgaggg atcgcgagcg caccgagcag gcgatcaagc ggacagccga
     1321 ggtcggcggc aatttcgacg ttgctttcag ggttggccga acgccgggat ccggtcagtg
     1381 gatgagattg cgaggcggcc tcgtcaagga tgacgccggg gtttcgcgcc atcttagcgg
     1441 gattgttctc gatatcgacg aggagaagca actcgaggag gcgctgagaa cgcgggaaag
     1501 ccacctgcgc tcgatcctcg acacggtgcc ggatgcgatg atcgtgatcg acggccaagg
     1561 tatcgtgcaa tttttcagca ccgcggccga aagactgttt ggctacaccg aacaggaggc
     1621 catcggcaag aacgtcagcg ccttgatgcc caacccggat cgggaacgtc acgacggcta
     1681 tcttgcgcgc tatcgatcct cgggcgaacg gcacatcatc ggcattggcc ggatcgtgac
     1741 cggccagcgc agggatcgaa ccatctttcc gatgcatctc acgatcggcg agatgcagtc
     1801 cggcggggaa ccctacttca ccggcttcgt gcgcgacctc accgagcacc agcaaaccca
     1861 ggcacggctc caggaactgc agtccgaact ggttcacgtc tctcgcctga gcgcgatggg
     1921 ggagatggcg tcggcgctgg cgcacgaact caatcaaccc ctcgccgcca tcagcaacta
     1981 catgaaaggc tgccgccggc tattggccgg cagcaccgat ccaaacaccc aaaaaatcga
     2041 aaatgccatg gaccgtgccg ccgaacaggc gttgcgcgcg ggccagatca ttcgtcgcct
     2101 gcgcgacttc gtatcacgag gggaatccga gaagcgggtc gagagcctcg caaaactgat
     2161 tgaagaggca ggcgcgctcg ggctggccgg cgcccgggag cagaacatac agcttcgctt
     2221 caatctgaac ccggaggtcg atctggtcct ggtggatcgg gtccagattc agcaggttct
     2281 ggtcaatctg ttccgcaatg ccctggaggc tatggcgcaa tcgcggcaac gggagcttag
     2341 cgcatcgaac gccaaggtcg cggacgacat gatcgaaatc gcggtgtccg ataccgggac
     2401 aggattcaat gatgacgtca agcccaatct gtttcagact ttttttacga cgaaagagac
     2461 cggcatggga gtaggactgt ccataagccg ttcgatcatc gaagcccatg gcggccggat
     2521 gtgggccgaa aacaatgctg cgggaggggc gacttttcgc ttcacattgc ccgccgcacc
     2581 aggtgagagt tgacagatgt cgagcaagac aaaggtttac gtgatcgacg acgacgaggc
     2641 gatgcgggat tcactcgatt ttctgctcgt ctctgccgat ttcaacgtga ccctgttcga
     2701 gaccgcgctg gattttctcg atgccctccc cgtccttgaa tttggatgcg ttgtctccga
     2761 cgtgcgcatg cccggcctcg acggcatcga gcttctgaaa cgcatgaagg cggccaacag
     2821 cccgtttccg attgtgatca tgaccgggca tggcgatgtg ccgcttgccg ttgaagcgat
     2881 gaaactgggg gcggccgatt ttctcgagaa accgttcgaa gatgaccgcc tgatcggcat
     2941 gatcgaggcg gccatccggc aggcggagcc ggcggcgaag agcgaggtgg tcacgctcaa
     3001 catcgcggca cggatagcca ctctcagccc gcgcgagcgg caggtgatgg aaggcctcat
     3061 cgccgggctt tccaacaaac tgattgcgcg cgactacgac atcagccccc gtaccattga
     3121 agtctaccgg gcaaacgtga tgacgaagat gcaggccaac aacctctcgg aattggtgcg
     3181 gcttgcgatg cgcgccggga tgctcaacga ttgaggtaaa tcaagccgcg actgtcatga
     3241 cgctgttatg ttggacaatg ccctactcag aaacagcacc agatgcgcgg gttttcgtca
     3301 tggcttcact cgcaaagccc acagtctacg tggtcgacga cgacgccgat gttctcggtt
     3361 cgttgcggtt tcttcttgaa gcggatggat tcgcggtacg aacgttcagg aacggcccgg
     3421 cgctgctgaa cgccgtccgc ttgaccggcg tcgactgctt cgtgatcgac tacaagatgc
     3481 ctgatatcaa tggcatcgat ttggctagcc gtttgcgtaa ccgcgatatt accgcaccga
     3541 ttatcctcat tacgggttat ccggacgaaa atatctcggc aagggcttca gccgcgggca
     3601 tccggcatgt agtgcagaaa cctctgcttg aggaaagcct tctgacgcat attcgcgggg
     3661 caattcaggg ggatcacccg gtcactggct aggaacggag ccatgacctc cggtatagta
     3721 cgtaagtaaa cccccttaag atatcgcacg aaatttccaa gccccgcttt cccaactagc
     3781 aattggccat ccgccgcaca ggagatggct catgctcacc caaccgctca gcacttccgt
     3841 tatcggcagc aagatcggcc gcgttgcctg ccccaccccc gcttcggacc agtttggtgc
     3901 gatcaccggg cacgccggcc tcgtcgccac ggagttcacc tacaaaaaag atgaagaaat
     3961 ctacggggag gatgagcctt cggaatatgt ttaccaggtg atcagcggcg cggtgcgcag
     4021 ctacaaactg ctatccgacg gacggcgcca gatcggcaca tttcatctgc ccggcgacgt
     4081 cttcgggctt gaatcgggag cggtccatcg gctggcagcg gaagccatta tcgataccac
     4141 cgtgcgcctg gtgaaacgcc gcagtctcga gcaggccgcc ggggtcgatg tcaatgtcgc
     4201 gcgcagcctt tggaccatga ccgctggcga gcttcgacac gcggaagatc acatgctgct
     4261 gctcgggcgc aagaccgcga tggagcgcgt tgccaccttc cttttggaaa tggatcgccg
     4321 gcttgcggtc gcgggaatga tggcgctgcc gatgtgccgc cgggatatcg gcgattatct
     4381 cggcctcacg ctggaaaccg tctcgcgagc gctttcgcag ctgcacgcgc aaggggtcct
     4441 cggattttcc ggcgcgcggc agatcgtgct ccgcaatcgt cagcggctgc gcaacatgga
     4501 tgcgtgagaa tgacagctat gtagtgcgcc gcgtttccgg catcacaagc caaatcaata
     4561 ccaggcccgt cgccgcgacg gcggcaagcc cggtgaaagc gatactgctg ccgaacctgt
     4621 cgctgacgta gccggccagc acggtgctaa gggaagcacc gatgccggtt gccgtgccga
     4681 cgatgccctg ggcgagattg aagtggccgc ttccaaacgc gacgtcggcg ataatcaacg
     4741 gcaccatgac gctaaagacg gccgcggtga tgccatcgaa cacctgcacc gcgaccagca
     4801 aatacggatc gctcacggtg gcgaacagca cgccgcggat cgccagcgtg gcgaagccca
     4861 gcagcagcag cggccgacgc ccccattgct gggccttgcg gccgaccgac ggagacgtca
     4921 gtgcgacgat ggcttgcggc acgacgatgc atgcggcgat cagcaccggc gcccactggc
     4981 tggaccgggt ggtaacgacg cccgccatca acgggagcat cgccgcgttg gcgagttgca
     5041 gcaagaacac gctgccggcg aagatcaaca gggggcgccg gcacatcaga tttagaacgc
     5101 tcgtcgcgtt ggcgtcgggc acctcgcgcg gcaccgcgcc atgcgcctgc gcggcatcga
     5161 tctcccattc gcggattcgc gccagcgcga gcaaggtcgg aatcgccaga atgaaggtga
     5221 cgagaaacac cgagcgactc gagagcagat agccgcacgt gcccatcacg gccgcggcga
     5281 cgccgttgcc gagtgacgca tagcgggcgt tgcgtccgaa ccgctcgccg atcgccagcg
     5341 gtccgaccag gccgaggctg atcgcggcaa tcgccggacc aagcacgcaa cttgcggcgg
     5401 cgtgcagggt cgcggcgacc accaccaccg gaaagatcgg ccaggcggca tatccgagcg
     5461 cactgacgcc aatcgtcgcc accgcaagag cggccaccag ccgctcggag cgggcggcgt
     5521 cgacgatggc gccgccgggc atttgtccga tcagcccgac gaggccgccg atggaaagca
     5581 caagaccgat ctcgacctgg gtccactttt gcgttgtcag gtaaaccgcg atgaatggac
     5641 cgaatccggt ctgcacatcc gcgagaaaaa agatgaacca atcaagtccg cgcaagctct
     5701 ggcgcgaggg agttggcgcc gccgcaggcg ccttcggctc tgaaacgaac ggcgcaacgc
     5761 tttcagcacc gaccggccgc tcacggcggt cacgatcaat tgggtcgcgc gaatgtcggg
     5821 atgaacgtgc gaacaggata tctctccgcc cttaaggacc aaactgcagt ggctgcaggc
     5881 tccctgatgc acccagcacg acgatcggcg tatcctcctt gtactctggc gccgccgtca
     5941 cctgatcctt ggtcaattcg agcgtgatac tgtcgctctt gttggcgatg cgtccgaaat
     6001 gcagcgcatt ccagtcgacg acgattttcc ggctgccgac gccgagaaaa ccgccgaagt
     6061 cgatcacggc cgcgcgcacc gaaccgctcc gatcgacgat tatatcgacg atccgcccca
     6121 tattctcgtc cgccgcgctg cggacctcgc ggccgagcac gccatgggca tccgccgccc
     6181 ggatgatggt gacggagggt ggcggcaccg cctccttgga tgggaccgcg ggggcggtag
     6241 tcggcggtgg ggcgtcgcga cccgcttgcg gcaaaccttt gtcatcggct gcccaggata
     6301 ttgcggccgt cgacgcgaca acggcgatcg cgatgaacat tgagagacgt tttgcgcgca
     6361 taaggactcc tgaagcgctt gatcgaaggg cataaattga cttgcggagg cctgacacaa
     6421 gcagctggcc ggtcggcttc caggtggaac ccaatgacgc ccgggaccaa tccggatcga
     6481 ccctcaaagt tcgaatcttt tgcgcatcaa atctcacgga cgccgataaa tgtccgcgcg
     6541 atcagtgcgc gagcacgatg gacacctgga tctggccgcg cgtacgcaaa acttccagcg
     6601 acacgtcatc gccgtggcga cggacccgca gatcgacgct cgaaggaccc agttgcaggt
     6661 cgcgcaggaa cacctcgtta aggaatgccg gcaggcgtgg attgcgcagg cgaatttcgc
     6721 cgcgctgggc gtcgaattcg agaccgagtg ctgcttccag cagggtgaac ggcgtcgcgc
     6781 ttgcccaggc ttgcggcgcg caagcgaccg ggtacagcgt cgggccgcgg cgcctttcac
     6841 gctgaaatcc gcagaacaat tcgggcagcc gccgcaaatc catatagctc
//
LOCUS       DASWGM010000333        11507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_440028,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000333 DASWGM010000000
VERSION     DASWGM010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440028"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VGA15_09580"
     CDS             complement(<1..1058)
                     /locus_tag="VGA15_09580"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9587982.1"
                     /translation="MPEAYIYDHVRTPRGRGKADGALHEVTALALATVPLKALKERNN
                     LAEDVVDDVILGVVDPVGEAGSDIARFAALKAGLGEAVPGVQISRFCASGLDAVNFAA
                     AQIMSGQHELTIGGGAESMSRVGIGASGGAWPMDPSIAVPAYFMPQGVSADLIATKYG
                     FSRDDVDAYAVQSQQRAAKAWDEGRFKNSVVPVKDVNGLTILAKDEHMRPTTTMQSLG
                     QLQASFAAVGAMGGFDAVAIQSHPEIERINYVHHAGNSSGIVDGAGAVLLGSKEAGAK
                     HGLKPRARIRAFTNIGSEPAMMLTGPVDVTEKLFERSGMKKSDIDLFELNEAFASVVL
                     RYIQAFDIDNAKINVNGGA"
     gene            complement(1148..2938)
                     /locus_tag="VGA15_09585"
     CDS             complement(1148..2938)
                     /locus_tag="VGA15_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9587983.1"
                     /translation="MPTYKAPLEDVSFLLNDVFQIDRYDNLPGFSDASADVREAVLNE
                     AAKLSEQVLQPLNRVGDLEGCIRHDDGSVTTPKGFKEAYKQVAEGGWLGLSAPTEYGG
                     QGLPVTLSQTVTEFQCSANMAFSMYGGLTMGATAALIVHGKPEQKAMFVPKMVAGEWT
                     GTMNLTEPQCGTDLGLLRTKAVKQADGSYKISGTKIFISAGEHDLTENIIHLVLARIE
                     GAPSGIKGVSLFVVPKILVNTDGSLGERNGVTCGSIEHKMGIHGNSTCVMNYDSATGW
                     LIGEENKGMQGMFVMMNEARLGVAVQGLAQSEVAYQNAVAYARDRLQGRSLSGAKAPD
                     KPADPIIVHPDVRRTLLTIRAFNEAARAMVVWTALKSDVAHRSSDPKDRQEADDHMGL
                     MTPVMKGVLTDVGFSNAVLAQQVFGGHGYIAETGMEQFVRDARIAMIYEGANGIQALD
                     LVGRKLPRDGGRAVMAFFGEVAAFAKDHGSDEAMKPYVAPLSTALGHLQQATTWLMQN
                     AMMKPDNAGAGATDYMQLFGLVTFAYMWARMAKVALDKIAASGATPYLTTKLVTGRFF
                     MERMLPETALHLARIQTGSATTMELAAEAF"
     gene            3306..6590
                     /locus_tag="VGA15_09590"
     CDS             3306..6590
                     /locus_tag="VGA15_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587984.1"
                     /translation="MNSRLSWSAEGIDPSVRERAEAAARRAGMSLSDWLNSTVGDFTP
                     PDFRSPTDQPPVPASRETPDVLDIHQRLDSITRQIEQISRPAPHSEALRNDAVRSESA
                     VVARQLNEAISRLDARLSQMSNPLPAKPAKLADDQLDLVERAAAQVYRPSPPISPASL
                     DFAVAEIAARQNELDSAPRFLPPRSAPPMVPATPAGPDFSSLERHLIQITGQIEALQR
                     PDGIEQSIAAFRGELAEIRHAITEAMPRQAIESIENEIRTLSRRIDDTRQSGIDDQAL
                     AGIERALGEIREVLRSLTPAEQLAGYDDAIRNLGAKLDLILRASEDPSTVHKLESAIA
                     ALRAIVSNIASNDALARLSDDVHTLSSKVDQLARSEGPSDSFTILEQRIAALALTLES
                     REPPGPSESSEQLEGAVRALSDRLDRMPVGNDNASAFAHLEQRVSYLLERLEASTDHR
                     AGNLGRVEDGLQDILRQLENQQASFAAVAESSRRPAEPADSGVIDVVKRELSDIRFSQ
                     TEAGRHTQESLEAVHSTLGHVVDRLAIIEGDLRTVRVQPVAPQAETTPAPPSAPAAAP
                     AIATAQQPKPELPNPAASQSHFATAPREFQAGPIQLPALAAAAPKAISEILDQHAAAP
                     RAAIEPGLPPDHPLEPGTRPSGRVPSPSERIAASESAISEIAAGPREQSSTSNFIAAA
                     RRAAQAAAAAPPVDKPARGPLKDPARDKAKVTSQEASTVTSKIRSVLVGASVVIIVLG
                     TFKLAMTLLDGGSVPAQPATESSGEPAPSIQWPADDAAKPASPAPVMPSMTSPTPIGR
                     QSFDGSTPNASDGTASAEILPNPAIPSASPLSASDITGSVPVAQVAPIGRKLVMVRVP
                     PTERLPDAIGGPVLRTAALKGDPTAAYEIGLRFAEGKGVAQNLDEAAKWYDRAAQAGV
                     VPAVFRLGTFYEKGLSVKKDIDIARRYYMQAAERGNAKAMHNLAVLDADGGGKGANYK
                     DASQWFRKAADRGVADSQFNLGILYARGIGVEQNLAESFKWFSLAAAQGDADSIRKRD
                     DIAKRLDAQSLAAAKLAIQTFTREPQPDDAVNVASPVGGWDVAPAQAGVAKPAAKPVL
                     TKRADR"
     gene            6817..7743
                     /locus_tag="VGA15_09595"
     CDS             6817..7743
                     /locus_tag="VGA15_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9587985.1"
                     /translation="MQLYLPIADIPVNVFLILAMGAAVGFVSGMFGIGGGFLMTPLLI
                     FIGIAPAVAVASVASHIAASSFSGAISYWRRRAIDPALAVVLLSGGTIGTALGVWTFT
                     LLRSLGQLDLMIALSYVVLLTAVGGLMFWEGLRAILRTRRGMAVPMRRSGSHGWIHGL
                     PLKMRFKRSKIYLSVIPVVVVGLIIGFIGAVMGIGGGFILIPIMIYLLRVPTSTVIGT
                     SMVLTLVTMVFATMLHAVTNHLVDAVLALILMVGGVTGAQFGARAGQRIRGEHLRLLL
                     GLLILAVGIRFAIELVIRPDDLFTIRETGGAG"
     gene            7740..8531
                     /locus_tag="VGA15_09600"
     CDS             7740..8531
                     /locus_tag="VGA15_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEX9587986.1"
                     /translation="MIARISLAAVGLGLALSAAFALSPAHAERLIVSVSNHRVTVTPN
                     YSGEELVLFGSVEKDATTPSTRTNYDLVVTVSGPRADMVTRRKERKFGIWINTDYRQF
                     LGVPTYLALFANRPFDAIASPEVQRRQQLGLNNVLLTQRVGPDYADVVPNDAFRSAFV
                     RLRSEHGLYREATSAVTFLTPTLFRTGIPLPAEVPIGTYDVEIKLFSDGALVTRAESA
                     FEIVKVGFEQFVANTARQSGLVYGLVTAFMALMTGWMASIVFRKD"
     gene            complement(8670..10025)
                     /locus_tag="VGA15_09605"
     CDS             complement(8670..10025)
                     /locus_tag="VGA15_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX9587987.1"
                     /translation="MANRKKTDRKNDNKNNTKSGGLKPTPRTAPRQPFSLRVLLKALG
                     PGLITGASDDDPSGIGTYSQAGAQLGYGIGWTMLLTFPLMAAIQEISARVGRVTGHGI
                     AGNVCRYYSVLLLNVVVTLLFIANTINIAADLGAMADATKLLIGGHGIIYVVAFGATS
                     VVAQIFLDYKRYVSVLKWLTLSLFAYVGALAFAHISWGQALAGVLVPRITWSADYFTT
                     IVAIFGTTISPYLFFWQASQEAEDQRVDAKKHPLIERQYGAQKEFHRIRADTVVGMAF
                     SNLIALSIIVTAAATLHAAGKTDIQTSAQAAEALRPLAGTFAEVIFALGIVGTGLLAI
                     PVLAGATAYAVGEGRKWPVGLARKPKEAVAFYSVLALSAAIGIALNFTSINPISALYW
                     SAVVNGVLAVPVMVLLMIMSRRKVVMGRFVIGGPLYWLGWLSTAAMLLSVVAMGVGLF
                     L"
     gene            complement(10161..11069)
                     /locus_tag="VGA15_09610"
     CDS             complement(10161..11069)
                     /locus_tag="VGA15_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9587988.1"
                     /translation="MIELRQFRQFIAVAEELSFRRAADRLHMAQPPLTVTIKQIEEEL
                     GTLLIERTNRIARLTPAGRVFLEECKRTVIQAERAIVAARRAGAGLNGALRVTFVASA
                     AREILPPILRAFREQYPEVELMLTEAMTAQQVTKIQTDDADVGFVIPPLRDADGLSVE
                     VLAKNQMVAALPENHPLAQLQSISLSDLASEDWILFPERQGPGLHERVLTACAQAGFV
                     PRVGQQASQMDTIASLVAGGMGVGLVTRIIATANRPGVVFRDLTGIGTPVEYELAIAY
                     GRSTPLVDAFMAVIRSRANWVGSAAN"
BASE COUNT         2061 a         3791 c         3584 g         2071 t
ORIGIN      
        1 gcgccgccat tgacgttgat cttggcgttg tcaatgtcga aggcctggat atagcgcagc
       61 accaccgagg cgaacgcctc gttcagttcg aacaggtcga tgtcggattt cttcatgccg
      121 gagcgctcga acagcttttc agtgacatcg accggaccgg tcagcatcat cgccggctcc
      181 gagccaatat tggtgaaggc gcggattctt gcacgcggct tcagaccgtg cttcgcaccc
      241 gcttccttgc tgccgagcaa gaccgcgccg gcgccgtcaa cgatgccgga ggaattgccg
      301 gcgtggtgca cgtagttgat gcgctcgatt tccggatgcg actggatcgc caccgcatcg
      361 aagccgccca tcgccccgac cgccgcgaac gaagcctgca actggccgag cgactgcatc
      421 gtcgtggtgg gccgcatgtg ctcgtccttg gcgagaatgg tgaggccgtt gacgtccttc
      481 accggcacca ccgaattctt gaagcggccc tcgtcccacg ccttcgcggc gcgctgctgg
      541 ctctgcaccg cgtaggcatc gacgtcgtcg cgcgaaaaac cgtacttggt ggcgatcaga
      601 tcggccgaca cgccctgcgg catgaaatag gccggcaccg cgatcgaggg atccatcggc
      661 caggcgccgc cagaggcacc gatgccgaca cggctcatcg actcggcacc gccgccgatg
      721 gtgagttcat gctggccgct catgatctgg gcggcggcga aattcacggc atcgaggccg
      781 gaggcacaga agcggctgat ctgaacgccg ggaacggctt cgccgagacc cgccttcagc
      841 gccgcgaagc gcgcgatgtc ggagccggcc tcgcccaccg gatcgaccac gccgagaatg
      901 acgtcgtcga cgacgtcttc ggcaagattg ttgcgctcct tgagcgcttt cagcggcacg
      961 gtcgccagcg ccagcgcggt cacctcatgc agcgcgccat cggccttgcc gcggccgcgc
     1021 ggcgtgcgaa cgtgatcgta gatatatgcc tcaggcatga cgccctcctg gtatgaggat
     1081 cataatatat aggcgaatgg gaagatgtcg cggatctagt caaaggacgt tgggaagatt
     1141 tctccggtca gaacgcttcc gccgccaatt ccatggtggt cgcgcttccg gtctggatgc
     1201 gcgcaagatg cagcgcggtt tccggcagca tccgttccat gaagaagcgg ccggtcacca
     1261 gcttggttgt cagatagggc gtcgcgccgc tggccgcaat cttgtccaat gctactttcg
     1321 ccatccgcgc ccacatatag gcgaaggtga cgaggccgaa caattgcatg taatcggtgg
     1381 cgccggcacc ggcattgtcc ggtttcatca tcgcattctg catcagccag gtggtagcct
     1441 gctgcagatg gccgagcgcc gtgctgagcg gcgcgacata gggcttcatc gcttcgtcgg
     1501 agccatgatc cttggcgaac gctgcaacct cgccgaagaa ggccatcacg gcgcggccgc
     1561 cgtcgcgcgg caatttgcgc ccgacgaggt cgagcgcctg aatgccgttg gcgccctcat
     1621 agatcatggc gatccgggca tcgcgcacga actgctccat gccggtttcg gcgatatagc
     1681 catggccgcc gaacacctgc tgcgccagca ccgcgttgga aaatccgaca tcggtcagaa
     1741 cgcctttcat gaccggcgtc atcagcccca tgtgatcgtc ggcctcctgg cgatccttgg
     1801 ggtcgctcga tctgtgggcg acgtcgcttt tcagcgcggt ccacaccacc atcgcgcggg
     1861 ccgcctcgtt gaaggctctg atcgtgagca atgtgcggcg cacatcggga tgcacgatga
     1921 tcggatcggc cggcttgtcc ggcgccttcg cacccgaaag cgagcggccc tgcagccgat
     1981 cgcgggcata ggcgacggcg ttctgatagg cgacctcgga ttgcgccagt ccctgcaccg
     2041 cgacgcccag ccgcgcctcg ttcatcatca cgaacatgcc ctgcatgccc ttgttttcct
     2101 cgccgatcag ccagccggtg gcgctgtcgt aattcatcac gcaggtggaa ttgccgtgaa
     2161 tgcccatctt gtgttcgatc gagccgcagg tcacgccgtt gcgctcaccg agcgatccgt
     2221 cggtattgac caggatctta ggcaccacga acagcgacac gcccttgatg ccgctgggcg
     2281 cgccctcgat tctggccagc accaggtgga tgatgttttc agtgagatcg tgctcgccgg
     2341 ctgagatgaa aatcttggtc cccgatatct tgtagctgcc gtcggcctgc ttgacggcct
     2401 tggtccgcag caggccgaga tcggtgccgc attgcggctc ggtcaggttc atggtgccgg
     2461 tccattcgcc ggccaccatc ttcggcacga acatcgcctt ctgttcgggc ttgccgtgca
     2521 cgatcagcgc cgccgtcgcg cccatggtca ggccgccata catcgaaaac gccatgttcg
     2581 ccgagcactg gaattcagtg acggtctggc tcagcgtgac cggcaggccc tgcccgccat
     2641 attcggtcgg cgccgaaagc ccgagccagc cgccctccgc cacctgcttg taggcttcct
     2701 tgaaaccttt cggggtggtc acgctgccat cgtcgtggcg gatgcagccc tcgagatcgc
     2761 cgacgcgatt gagcggctgc aacacctgtt cgctgagttt cgcagcctcg ttcaaaacag
     2821 cctcccgcac gtcggcggag gcgtcgctga aaccgggcag attgtcgtag cggtcgatct
     2881 ggaacacatc gttgagcagg aagctgacgt cttccaaggg ggctttgtag gtcggcatga
     2941 tgtcctctcg gctcgctggc gggcctcgtt taaggaagat tttgcagcgc agcaatcgtc
     3001 cggttgcaga aagccgacct tagacggttc cggacgtcca ggcagccgta aggcagctat
     3061 cgcgccgcca ttgcgacaaa ataaccgccg cgcccggcgg cggccgcgcg ccaaacggtc
     3121 aattttgctc gccacgcaac tttcagctcc tgcgggctga ttctttaacc cgttctttac
     3181 cgtatcacga aaaagtcgat gccagaggca gacgacctgc gcggaattcg attgtctcct
     3241 cacgagggac gcgcggggag gctgaaggcg caggtgtcgc gccggattcg gaaccggacg
     3301 agagcatgaa ttcgcgccta tcgtggagtg cagagggcat cgatccatcc gttcgcgaga
     3361 gggccgaggc cgccgcgcgg cgcgctggca tgtcgctcag cgactggctc aattccaccg
     3421 tcggtgattt cacccctccc gactttcgta gccccaccga tcaaccgccg gtgccggcga
     3481 gccgggaaac ccctgatgtc ctggacattc atcagcggct ggattcgatc acgcgccaga
     3541 tcgagcagat ctcgcgcccg gcgccgcaca gcgaggcgct tcgcaacgac gccgtgcgca
     3601 gcgagtcggc cgttgtcgcc cggcaattga acgaagccat ctcgcgcctc gatgcgcggc
     3661 tgtcgcagat gtccaatccc ctgccggcga aaccggccaa gcttgcggac gaccagctcg
     3721 atctggtcga gcgcgcggct gcccaggtct atcgcccctc tcccccgatc agccccgcgt
     3781 cactcgactt cgcggtcgca gagatcgccg cacgccagaa cgaactcgac agtgcaccac
     3841 gattcctgcc gccgcgcagc gcgccgccga tggtgcccgc aacgccggcg gggccggact
     3901 tttcctccct cgagcgccat ctgatccaga tcaccggcca gatcgaagcg ctgcagcgtc
     3961 cggacggcat cgagcagtcg atcgccgcat tccgcggcga attggccgaa attcgtcacg
     4021 ccattaccga ggcgatgcca cgccaggcga tcgaatcgat cgaaaacgaa atccgcacgc
     4081 tgtcccgccg cattgacgac acccgtcaga gcggcatcga cgaccaggcg ctggcaggca
     4141 tcgaacgcgc gctcggcgaa attcgcgagg tgctgcgctc tctgacgccc gcggagcagc
     4201 tggcgggcta cgacgacgcg atccgcaatc tcggcgccaa gctcgacctg attttgcgcg
     4261 ccagcgaaga tccctcaacg gtccacaagc tcgaaagcgc catcgccgca cttcgcgcca
     4321 tcgtctccaa tatcgcgtcc aacgacgcgc tggcgcggct cagcgacgac gtgcacacct
     4381 tgtcgtccaa ggtcgatcag cttgcccgtt ccgaaggccc cagcgattcg tttacgatcc
     4441 tcgagcagcg catcgccgcg ctcgccttga cgctggaaag ccgcgagccg ccggggccaa
     4501 gcgaaagctc cgagcagctc gagggcgccg tgcgggcgct gtcggaccgc ctcgaccgca
     4561 tgccggtcgg caacgacaac gcctcggcct tcgcccatct cgaacagcgc gtgtcttatt
     4621 tgctcgaacg cctggaggcg tccaccgatc accgcgccgg caatctcgga cgggtcgagg
     4681 acggattgca ggacattctg cgccagctcg aaaaccagca ggcgagcttt gccgcggtgg
     4741 ccgaaagcag ccgacgcccg gccgagccgg ccgacagcgg cgtgatcgat gtcgtcaagc
     4801 gcgaattgtc cgacatccgc ttcagccaga ccgaagccgg ccgccatacc caggaatccc
     4861 tcgaagccgt ccacagcacg ctcggccatg tggtcgatcg gctggcgatc atcgaaggcg
     4921 atctgcgcac ggttcgcgtc cagccggttg cgccgcaggc ggaaacgacg ccggcacctc
     4981 catccgcacc agcggccgcg cctgccatcg cgacagcgca gcaaccgaag cctgaattgc
     5041 cgaatccggc tgcgtcgcag agccatttcg ctacagcccc gcgggaattc caggccgggc
     5101 cgatccagct gccggcgctc gccgctgcgg cgccaaaggc aatcagcgag attctggacc
     5161 agcatgcggc ggcaccgcgc gccgcgatcg agccgggcct gccgcccgat cacccgctcg
     5221 aaccgggaac acgtccttcc gggcgggtgc cgtcgccgtc ggagcgcatt gccgcgtccg
     5281 aaagcgcgat cagcgaaatt gccgcgggcc cgcgtgagca aagcagcact tcgaatttca
     5341 ttgccgccgc gcgtcgtgcc gcccaggccg ccgcggccgc gccgcctgtc gacaagcccg
     5401 cccgcggccc actcaaggat cccgcccgcg acaaggccaa ggtgacatcg caggaggcat
     5461 cgacggtcac gtccaagatt cgctcggtgc tggtcggcgc cagcgtggtg atcatcgtgc
     5521 tcggcacttt caagctggcg atgacgctgc tcgacggcgg cagcgttccg gcgcagcctg
     5581 cgacggagag ttcaggcgag ccggcgcctt caattcaatg gcccgcggat gacgccgcca
     5641 agccggcgtc accggcaccg gtcatgcctt cgatgacgtc gccgacgccg atcggccggc
     5701 aatcgttcga tggctccacg ccgaatgcct ccgacggcac cgcgtcggct gaaatcctgc
     5761 cgaacccggc gataccatcc gcttcgccgc tctccgccag cgatatcacc ggctcggttc
     5821 cggtggcgca ggtggcgccg atcggtcgca aactcgtaat ggtacgggtc ccgccgacgg
     5881 aaagattgcc ggacgccatc ggcggccccg tattgcgcac tgccgcgctg aagggcgatc
     5941 ctacagccgc ctatgagatc ggcctgcgct ttgccgaagg caaaggcgtt gcacagaatt
     6001 tggatgaggc tgcgaaatgg tatgaccgcg cggcgcaagc cggcgtggtg ccggcggtct
     6061 tcaggctggg gaccttctac gaaaagggtc tcagcgtgaa aaaggacatt gatatcgcgc
     6121 gccgctacta catgcaggcg gccgagcgcg gcaacgccaa ggcgatgcat aatctggccg
     6181 tgctcgatgc cgacggcggc ggcaagggcg ccaactacaa ggacgcgtcg caatggttcc
     6241 gcaaggcggc cgaccgcggc gtcgcggaca gccaattcaa cctcggcatc ctctatgccc
     6301 gcggcatcgg cgtcgaacag aacctggccg aatcgttcaa atggttcagt ctggcggcgg
     6361 cgcagggcga tgcggactcg atccgaaaac gtgacgatat cgccaagcgg ctcgacgccc
     6421 agtcgctggc tgcggccaag ctcgcgattc agaccttcac gcgagaaccg cagcccgatg
     6481 acgccgtcaa cgtggcgagc cctgtgggcg gttgggacgt cgcgccggcg caggcaggtg
     6541 tggcaaagcc cgcggccaag ccggttttga ccaaacgcgc cgaccgttaa tctgaatcgt
     6601 tcaccacact cgaaggcgcg cggccggctg cgtcaaaaaa gccgtttcaa gcagattctt
     6661 tagtccctcc tttggtcgaa ttcggttatg caggggcgcg gcacttgacc ccgcgttcgc
     6721 gcctatcccg tgtggacgaa tcccactgcc cgatcagaaa ccttgccgtg agcggtttcg
     6781 gccagcttca aggctaaatc caagaagcaa acgcgcgtgc agctttatct tccaatcgcc
     6841 gacattccgg tcaatgtttt cctcatcctg gcgatgggcg ctgcggttgg tttcgtgtcc
     6901 ggcatgttcg gcatcggcgg cggatttctg atgacgccgc tgttgatttt catcggcatc
     6961 gcgcccgcgg tcgcggtcgc ttccgttgcc agccacatcg cggcctcgtc gttctccggc
     7021 gccatctcct actggcgccg ccgtgccatc gatccggcgc tggcggtggt gctgttgagc
     7081 ggcggcacta tcggcaccgc gcttggcgtc tggaccttca cgctgctgcg ctcgctcggc
     7141 cagctcgacc tcatgattgc gctgtcctac gtcgttttgc tgaccgccgt cggcggcttg
     7201 atgttctggg aaggcctgcg cgcgatcctg cggacgcggc gcggcatggc ggtaccgatg
     7261 cgccgctccg gcagccatgg ctggatccat ggcctgccgc tgaagatgcg cttcaagcgc
     7321 tccaagatct atctgtcggt gatcccggtc gtggtggtcg gcctgatcat cggctttatc
     7381 ggcgcggtga tgggcattgg cggcggcttc atcctgatcc cgatcatgat ctatctgctg
     7441 cgggtgccga cctcgaccgt gatcggcacc tcgatggtgc tgacgctggt caccatggtg
     7501 tttgccacca tgctgcatgc ggtcaccaac catctcgtcg atgccgtgct ggcgctgatc
     7561 ctgatggtcg gcggcgtgac cggtgcgcag ttcggcgccc gcgccggtca gaggattcgc
     7621 ggcgaacatc tgcggctgct gcttggactt ttgattctgg cggtcggcat ccggtttgcg
     7681 atcgaactgg tgatccgtcc cgacgatctc ttcaccatcc gggaaaccgg aggcgccgga
     7741 tgatcgcgcg tatctcactt gcggcggtcg gccttgggct ggcgctgagc gcggctttcg
     7801 cgctatcgcc cgcgcatgcc gagcggctga tcgtctcggt ctcgaaccac cgggtgacgg
     7861 tgacgccgaa ctattccggc gaggagctgg tgctgttcgg ctcggtcgaa aaggatgcaa
     7921 ccacgccttc gacccgcacc aattacgatc tggtggtgac cgtctccgga ccgcgcgccg
     7981 acatggtgac gcggcgcaag gaacgcaaat tcggcatctg gatcaacacc gactaccggc
     8041 agtttctcgg agttccgacc tatctggcgt tgtttgccaa ccggccgttc gatgccatcg
     8101 cttcgccgga agtgcagcgg cgccaacagc tcggcctcaa caatgtgctg ctcacccagc
     8161 gcgtcggacc cgactatgcc gacgtggtgc cgaacgacgc gttccgcagc gcctttgtgc
     8221 ggctgcgctc cgaacatggc ctctatcgcg aggcgacttc ggcggtgaca ttcttgacgc
     8281 cgacattgtt tcgcaccggc attccgctgc cggccgaggt gccgatcggc acctacgacg
     8341 tcgaaataaa actgttttcc gatggcgcat tggtgacgcg agccgaatcc gcgttcgaaa
     8401 tcgtcaaggt cggattcgaa cagttcgtcg ccaacaccgc gcggcagagc ggcctggtct
     8461 atggattggt cacggccttc atggcgctga tgaccggctg gatggcctca atcgtgtttc
     8521 ggaaggattg aaggttcccc ggacgcggcg cagcgcgccg cactggcggc gtgatgcgct
     8581 gctgatccgg ggtccatttt gtttagttgc gaatgggtcc cggctctgcg gagcagcgtg
     8641 aagaacgctg caccgcgtcc gggacacgat caaagaaata accccacgcc catcgccaca
     8701 acgctgagca gcatcgccgc ggtggagagc cagcccagcc aatacagcgg gccgccgatc
     8761 acaaaacggc ccatcaccac cttgcgacgc gacatgatca tcagcagcac catcacaggg
     8821 acggcaagca cgccgttgac cactgcgctc caatacagcg ccgagatcgg attgatcgag
     8881 gtgaagttga gcgcgatccc gattgctgcc gacagcgcca gcaccgaata gaacgcaacc
     8941 gcctccttcg gcttgcgcgc gagtccgacc ggccatttcc gcccctctcc cacggcgtag
     9001 gcggtggccc ccgcgagcac cggaatggcc aacagcccgg tgccgacgat gcccagcgca
     9061 aaaatcactt ccgcgaatgt tccggcgagc gggcgcagcg cttcggccgc ttgcgccgag
     9121 gtctggatat cggttttgcc ggcggcatgc agcgtcgccg cggcggtgac gatgatcgac
     9181 agcgcgatca ggttcgagaa tgccatgccg acgacggtgt cggcgcgaat gcgatgaaat
     9241 tccttttgcg cgccatattg ccgctcgatc agcggatgct ttttggcatc gacgcgctga
     9301 tcctcggctt cctgcgacgc ctgccagaaa aacagatagg gcgagatcgt ggtgccgaaa
     9361 atggcgacga tggtggtgaa atagtccgcg ctccaggtga tgcgtgggac aagaacgccg
     9421 gcaagcgcct ggccccagga aatgtgcgca aaggcgagcg ccccgacata ggcgaacagg
     9481 ctcagcgtca gccatttcag caccgacacg tagcgtttat agtcaagaaa gatctgcgca
     9541 acgaccgacg tcgccccgaa cgccacaacg taaataatgc cgtgcccgcc gatcaggagt
     9601 tttgtcgcat cggccatcgc gcccagatcg gccgctatgt tgatggtgtt ggcgatgaac
     9661 agcagcgtta ccaccacgtt gagcagcagc accgaataat accggcagac gttgccggca
     9721 atgccgtgcc cggtgacgcg tcccaccctt gccgagattt cctggattgc cgccatcaac
     9781 gggaacgtca acagcatggt ccagccgatg ccgtagccga gctgcgcgcc ggcctggcta
     9841 taggtgccga tgccggacgg atcgtcgtcg gaggcgccgg tgatcaatcc cggtccgagc
     9901 gctttgagca agacgcgcaa gctgaacggc tggcggggcg cggtcctcgg cgtcggtttc
     9961 aggccaccgg acttcgtatt attcttgttg tcgttcttgc gatcggtctt tttgcgattt
    10021 gccatgccca ggtccagccg ccgcgacaac gatcccgaca ggtttcggtt ccgggcggcg
    10081 aacttgcccg agggcgcccc gcgcggcaag caaacccggc gtggagctta tcaacggcgc
    10141 acgcggcttt ggcgcgaaag ctagttggca gccgatccca cccagttcgc gcgggatcgg
    10201 atcaccgcca tgaacgcatc caccaggggc gtcgagcggc cataggcgat cgccagttcg
    10261 tattcgaccg gcgtgcctat tccggtgaga tcgcgaaaca ccacgcccgg ccggttggcg
    10321 gttgcgatga tgcgggtgac gagcccgacc cccatgccgc cggccaccag actggcgata
    10381 gtgtccatct gcgaggcttg ctggccgacc cgcggcacga aaccggcctg cgcgcaggcc
    10441 gtgagcacgc gctcatgcag gcccggcccc tgccgctccg gaaagaggat ccagtcttcg
    10501 gacgccagat ccgacaacga aatcgattga agctgcgcga gcggatggtt ttcgggaagt
    10561 gccgcgacca tctggttttt cgccagcacc tcgacgctta gcccgtcggc atcgcgaagc
    10621 ggaggaatca cgaagccgac gtcggcgtca tcggtctgga tttttgtgac ctgctgcgcc
    10681 gtcatcgcct cggtgagcat cagctcgact tcgggatatt gctcgcgaaa cgcacgcagg
    10741 atcggcggca ggatttcgcg ggccgcgctc gccacaaatg tcacccgcag cgcgccgttc
    10801 aatcccgcac cggcgcggcg cgccgcgaca atcgcgcgtt cggcctggat cacggtgcgt
    10861 ttgcattcct cgagaaacac ccggccggcg ggggtcaggc gcgcgatgcg attggtgcgc
    10921 tcgatcagca gcgttcccag ttcttcctcg atctgcttga tcgtgaccgt gagcggcggc
    10981 tgcgccatgt gcaaacgatc ggcggcgcgg cgaaaactga gttcttctgc gacggcaata
    11041 aactgtcgaa actgacgcaa ctcgatcatg ccctggcccg acgtccccca tttcaattgt
    11101 gaacagggta tcggatcaag gcaaaaaaca cgaacgaatt caggatcgcg agcaggctag
    11161 gcgccggttc ggatcgctcg aggaaaatga gagcccccac cccggccttc ccccgcaagc
    11221 gggagagcgg gaagaaggtg tgtcgtgcga cttgagcaac cgccccggag ttgagcacca
    11281 actgcacaac gcgcaacggc ccgttccctc tcccgcttgc gggcgcttcg gaggctggcc
    11341 tggccttgcc ttgcttaggt aaccgtcaat ttcttcaccg gcgtgggatc gaggttgaaa
    11401 atgcgcgccg cgttcagccc gagaatgttg cgtttggcct gttcggtcaa aaacggcaga
    11461 tcgcagattt cctgcggcag gtcgaaatcg aaatgcggcc agtccga
//
LOCUS       DASWGM010000334         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1832000,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000334 DASWGM010000000
VERSION     DASWGM010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832000"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGA15_09615"
     CDS             <1..197
                     /locus_tag="VGA15_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587989.1"
                     /translation="GFDSAARQKDVGVADSMALLEQLLNQRRRTRAPYDVVAGADYRR
                     EVESFQPDFLRHSMNGRSRD"
     gene            complement(194..1024)
                     /locus_tag="VGA15_09620"
     CDS             complement(194..1024)
                     /locus_tag="VGA15_09620"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9587990.1"
                     /translation="MGKLDGKVAVITGAASGMGRATAIRFAKEGAAVVVADLNSQGGE
                     ATISEIAATGGRAVFQRTDVGVENDIKSLIDRAVKEYGRLDITYNNAGIGGATGRLTD
                     INAEDWDKTFVILTRAVFLGIKHSIEPMRKAGGGSIISTASVAGLKGAAGLHAYSAAK
                     AAVVNLTESAAIELGHDRIRVNCICPGGIVTPLVYRGMRGGEEEAQRNMARMQPIPRA
                     GRPEDIAAMALFLASDDAEWVTGTAMIVDGGMNTGNVRVKFGTGFSGPSFERPDRTNS
                     "
     gene            complement(1242..>1534)
                     /locus_tag="VGA15_09625"
     CDS             complement(1242..>1534)
                     /locus_tag="VGA15_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9587991.1"
                     /translation="LVLMDMQMPVMDGLAATRAIRAWETLHRLPATPVIVLTASAFGE
                     DVDQCLAAGATLHLAKPIKKAVLLATIRDLTADARGSLHASAVDELPSPSTK"
BASE COUNT          265 a          506 c          460 g          303 t
ORIGIN      
        1 tcgggtttga ttcggccgcc cgccagaaag acgttggcgt ggccgattcg atggcgctgc
       61 tcgagcagct tctcaaccag cgccggcgga cccgcgcccc atacgatgtt gtcgccgggg
      121 cggattatcg acgcgaggtc gagagcttcc agccggattt cctgcggcat tcgatgaacg
      181 ggcggtcgcg cgactagcta ttggttctgt cgggacgctc gaacgacggt cccgagaaac
      241 ccgtgccgaa tttcacccgc acgttgccgg tgttcattcc gccatcgaca atcatcgcgg
      301 taccggtcac ccattcggcg tcgtcgctgg caaggaacaa cgccatggcg gcgatatcct
      361 cgggccggcc cgcgcgcgga atcggttgca tccgcgccat gttgcgctgc gcctcttcct
      421 ctccgccccg catcccgcga tataccagcg gggtgacgat gccgcccggg catatgcagt
      481 tgacgcgaat acgatcgtgg ccgagttcga ttgccgcgct ttcggtgaga ttcactacgg
      541 ccgctttggc cgcgctgtac gcatgcaatc ccgcggcacc cttgagcccc gccaccgatg
      601 cggtcgagat gatcgacccg ccgccggcct tgcgcatcgg ttcgatcgaa tgcttgatcc
      661 cgaggaacac tgcgcgtgtc aggatcacga acgtcttgtc ccagtcctcg gcgttgatat
      721 ccgtgaggcg gccggtggcg ccgccgattc cggcattgtt gtaggtgata tcgagccggc
      781 cgtattcctt cacggcgcga tcgatcagcg actttatatc gttctcgacg cctacatcgg
      841 tgcgttgaaa gaccgcgcgt ccaccggtcg cggcaatttc cgagatcgtg gcctcgccgc
      901 cctgcgagtt aaggtcggcc accaccacgg cagcgccttc tttagcgaac cgaatcgcgg
      961 tagcgcgtcc catcccgctc gccgcgccgg ttatcactgc aacttttccg tccagcttgc
     1021 ccatagtagt ggctcctgta tctccgtatt tccctggccg ggtcttgcaa ccacgaggcg
     1081 tcgacatacg aatttacctc gaacgccgtt aatcgcgccg cgatggcgat gacccgctca
     1141 tcacgtttag cacaagcacc tcacggcatg gcagccaacg tgctgaatcg aaccgatgag
     1201 gcacgggaga agccacgtgc ccgcgacgcc agcggtggcg atcacttcgt tgacgggctg
     1261 ggcagttcgt caaccgccga agcgtgcaga gatcctctcg catcagcggt caaatcacgg
     1321 atggtcgcca gcagaacggc cttttttatc ggtttggcca gatgaagggt ggcgccggcg
     1381 gccaggcact ggtccacatc ctcgccgaaa gccgacgcgg tcaagactat aaccggggtc
     1441 gccggcagtc ggtggagcgt ttcccacgcg cggatcgcac gggtcgctgc cagcccatcc
     1501 atgaccggca tctgcatgtc catcaggacc aggt
//
LOCUS       DASWGM010000335         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2252295,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000335 DASWGM010000000
VERSION     DASWGM010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2252295"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VGA15_09630"
     CDS             <1..635
                     /locus_tag="VGA15_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9587992.1"
                     /translation="MQALIQKMREPHAADYAEKIATADRLLYRRGNFFGTVDQLICDA
                     LRNELNAEIALSPGFRWGSSMLPGQPVTMEDVLSETAITYPETYVQSLTGSQIKDILE
                     DVCDNLFNADPYYQQGGDMVRVGGMAYTCTPAESVGRRISELKLDSGRALSAGKSYRV
                     AGWASVNAQQGMPVWDVLAKYLRSGKAPDPRGAGVTLKGVEDNPGIAGQG"
     gene            954..1994
                     /locus_tag="VGA15_09635"
     CDS             954..1994
                     /locus_tag="VGA15_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9587993.1"
                     /translation="MIFRQLFDSVSGTYSYLLASRTGGEALILDPVLEKVDRYCQLLR
                     ELDLKLVKAVDTHLHADHVTGLGELRDRTHCVTIMGEQSKADVVAMRVADGDKVMIEG
                     LGLDVMYTPGHTDDSYSYLMGDRVFTGDTLLIRGTGRTDFQNGSARAQYDSIFGRLLK
                     LPDETMVFPAHDYKGDTVSTIGEEKRFNPRLQVRSVDEYVELMGNLNLPNPKLMDVVV
                     PANMHVGLHQDDLAKQGLAFSAREGIESLGRPDILLVDLRENSERAKHGMLPGALHAP
                     YPSIDESLSPGGMLREVAAATGRRIVFFCAFGERSAMAVAAAREAGLANTAHIAGGMD
                     AWKKAGGPVLTG"
     gene            2258..2491
                     /locus_tag="VGA15_09640"
     CDS             2258..2491
                     /locus_tag="VGA15_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9587994.1"
                     /translation="MDDEPKSAAELKEIRLNRKLAQDAEGIKAMADIAAADIAIRKRT
                     ENLRAIRLAKEAADRDKPAEPKAKAKPRKKKSL"
     gene            2492..2773
                     /locus_tag="VGA15_09645"
     CDS             2492..2773
                     /locus_tag="VGA15_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9587995.1"
                     /translation="MTAEDRNRADARFNKAARAASEAKTGKTDRDVAAKAVLDNMAKL
                     KALRLAREAAAPPPAATVKKTRAKPKKPGEKGPALADWLAGQQSGGRRT"
BASE COUNT          631 a          917 c          972 g          526 t
ORIGIN      
        1 cgatgcaggc gctgatccag aaaatgcgcg agccgcatgc ggcggactac gccgagaaga
       61 tcgccaccgc cgatcgcctg ctctatcgcc gcggcaattt ctttggcacc gtggatcaat
      121 tgatctgcga cgcgttgcga aacgaactca acgccgagat cgcgttatcg ccgggctttc
      181 gctggggctc aagcatgctg ccgggccagc ccgtgacgat ggaagacgtg ctgtccgaga
      241 ccgcgatcac ctatccggaa acctacgtcc agagcctgac cggcagccag atcaaggata
      301 tcctcgaaga cgtctgcgac aatctcttca atgccgatcc ctactatcag cagggtggcg
      361 atatggttcg ggtcggcggc atggcctaca cttgtacccc cgccgaaagc gtcgggcggc
      421 gaatttccga gctgaagctc gatagcggcc gcgcgctctc ggccggcaag agctacaggg
      481 tggccggctg ggcctccgtc aacgcgcagc agggaatgcc ggtgtgggat gtccttgcca
      541 aatatctgcg ttccgggaag gcgccggatc cacgtggcgc gggcgtcacc ttaaagggcg
      601 ttgaagataa cccgggcatc gcaggacaag gatgaagaat ttcctgggac atcgacggtc
      661 gttcgttcgc gcggtatgca acgccgtgat cgcagcgacg atggcggcaa cggtcccttc
      721 tgccgcccgc gccgggcagt ctggtcgcgc aaggcgtgcg gtttgatgcc tgcctcaata
      781 cccgcgggat gcgatcgagc gcgaaaccgg caagcggccg gagttcgtta ccgtcgcgac
      841 gccggtccag gtcggcgtcg ggcagatcct ggcgctgacc gaaaacggat atacgttggt
      901 tcggccgtag aatttctaag gtagtatcgg gaatcaattg gaggcgggag gccatgattt
      961 ttcgccagtt gttcgacagc gtttcaggaa cttacagcta tctgctggcc agccgtaccg
     1021 gcggcgaggc gctgatcctc gatcccgtgc tggagaaggt cgatcgctac tgccagctgc
     1081 tgcgtgaact cgatctcaaa cttgtgaagg ccgtcgatac ccatctgcac gccgaccacg
     1141 tcaccgggct cggcgagctg cgcgaccgca cccactgtgt caccatcatg ggcgagcaga
     1201 gcaaggccga cgtggtggcg atgcgggtcg ccgacggcga caaggtgatg atcgagggcc
     1261 tcggtctcga cgtgatgtac acgcccggtc ataccgatga ttcctacagc tatttgatgg
     1321 gcgatcgggt gttcaccggc gatacgcttc tgatccgcgg caccggccgc accgatttcc
     1381 agaacggcag cgcgcgggcg caatacgatt ccatcttcgg ccggctgctg aaactgcctg
     1441 acgagaccat ggtttttccc gcgcacgact acaagggcga tactgtttcc accatcggcg
     1501 aggaaaaacg cttcaatccg cggctgcagg tgcgctcggt ggatgaatat gtcgagctga
     1561 tgggcaatct gaatttgccg aatccgaagc tgatggatgt cgtggtgccg gcgaacatgc
     1621 atgtcggcct gcatcaggac gatctggcga aacaaggtct ggcgttcagc gcccgggagg
     1681 gaatcgagag cctcggccga cccgacattc tgctggtcga tctcagagaa aacagcgagc
     1741 gcgccaagca cggcatgctg ccgggcgcgc tgcatgcgcc ctatccttcg atcgacgaga
     1801 gcctttcgcc cggcggcatg ttgcgcgaag tggctgcggc gaccgggcgg cgcatcgtgt
     1861 tcttctgcgc gttcggcgag cgctccgcca tggccgtcgc cgccgccagg gaagccggcc
     1921 tcgccaacac cgcgcacatc gccggcggca tggatgcgtg gaagaaagcc ggcggcccgg
     1981 tgctgacggg ttgacgaaag ctgcgtcgtt tgccgtcaaa tccagcatgg taaaggccgg
     2041 ggcgacgttc tgatcggctt ggtgcgatgc ctgcgcgagc ggcttgagcc ggtcgcgcgc
     2101 gagcatccct tcaccacgat tgccgatttc ggctcaaaaa ggggcgcttt accggagatt
     2161 ctggccaaaa gatgcccctt tggcggtcta aggcgtggta ggatcgaaca aaaggtcgcc
     2221 gcccaatcgt gcgcgccgtg agtttttgag gtattcgatg gatgacgaac cgaagagcgc
     2281 cgccgaactg aaagagatcc ggctgaaccg caagctcgct caggatgccg aaggcatcaa
     2341 ggcgatggcg gatatcgcgg cagccgatat cgcgatacga aagcggaccg agaacctgcg
     2401 cgcgatccga ttggcgaagg aagccgccga ccgcgacaag ccggcggaac ccaaagccaa
     2461 agctaaaccc aggaagaaga agtcgctcta aatgacagca gaagatcgca accgcgccga
     2521 tgcgcgcttc aacaaggccg cacgcgccgc gagcgaagcc aagaccggca agaccgaccg
     2581 cgatgtcgcc gcgaaagccg tcctcgacaa catggcgaag ctgaaggcat tgcggctcgc
     2641 ccgcgaggca gccgcccctc cgcctgccgc aaccgtcaaa aagacgcgcg ccaagccgaa
     2701 gaagccagga gaaaagggcc ccgcattggc agattggctg gcgggacagc aaagcggcgg
     2761 gcggcggact tagtccgagc accagagcgt tttccagcga aagccggccc cggacttgat
     2821 ccggggtgga gaccggttcg cgtcaagaaa acgcgtcaaa tcaagaatct agagtcccgt
     2881 ttcgattcta tcgaaacggg aaagactcta gactggcgcc ggccattcga ccatcgatcg
     2941 cgtcttggtc gcggcatcgt aaacctgcac ctgaagcatc tggtactgat tcttcaaagc
     3001 catgcccgcc tcttcggcgg cctcgaccgt atcatggtgc gacttg
//
LOCUS       DASWGM010000336         5512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1832510,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000336 DASWGM010000000
VERSION     DASWGM010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832510"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /gene="boxB"
                     /locus_tag="VGA15_09650"
     CDS             complement(<1..833)
                     /gene="boxB"
                     /locus_tag="VGA15_09650"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX9587996.1"
                     /translation="MNVDYSTKIPNNVNLSEDRQVLKALEGWHPGYMDWWSDMGPEGF
                     QQSLVYLRTAYSVDPRGWAKFDYVKMPDYRWGILLAPQEENRVIPFGENFGKPAWQEV
                     PGEHRATLRRLIVIQGDTEPASVEQQRHLGKTAPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLQKYFGRDGREEADELLRRRSGDADSPRMLGAFNEATPDWLSFFMFTYFTDRDGKMQ
                     LHSLAQSGFDPLSRTCRFMLTEEAHHMFVGETGISRVVQRTVEAMKAAGISDPSDIAK
                     VR"
     gene            complement(990..2678)
                     /gene="boxC"
                     /locus_tag="VGA15_09655"
     CDS             complement(990..2678)
                     /gene="boxC"
                     /locus_tag="VGA15_09655"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEX9587997.1"
                     /translation="MAGEDRVLAGGAKHIDFQTDPSRYRHWKLDVDGEVATLTMDVDE
                     NGGLFEGYQLKLNSYDLGVDIELADAVQRLRFEHPGVKVVVVRSGKNRVFCAGANIRM
                     LAGATHAHKVNFCKFTNETRNGLEDSSENSGQRFVTVINGTAAGGGYELALATDHIIL
                     ADDGSAAVALPEVPLLAVLPGTGGLTRVVDKRKVRRDHADYFCTIEEGIKGKRAVQWR
                     LVDEIVPNSRLEAKVAERATEFAAASKRNGTGKGMTLTPLTRRFDDNGIRYGHVSVDI
                     DRAARIATISIQAPDAAPPADIDGLTAQGASFWPLQVARELDDAILHLRINELETAML
                     VFKSHGERAGVLACDAFLEANKAHWLVNEIRQYWKRVLKRIDVTSRTLVTLVEPGSCF
                     VGTLAELVFAADRSYMLIGSRQGDNRAPPTIELSAMNFGPYPMSHGLTRLQSRFQADA
                     EDVLRARKSIGEALDAEQAEALGLVTFALDDIDWDYEIRVFFEERTSFSPDSLTGMEA
                     NLRFVGPETMESKIFSRLTAWQTWIFQRPNAVGETGALRRYGTGQKAQFDMTRV"
     gene            complement(2816..3634)
                     /locus_tag="VGA15_09660"
     CDS             complement(2816..3634)
                     /locus_tag="VGA15_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9587998.1"
                     /translation="MQGLNSSGFLTIGTSDLEYRMIGPAPADAPTIVMLHEGLGCAGL
                     WGDFPDRLAAATGAGVLVYSRAGYGASTTVALPRPLDYMHIEALDTLPRLLDQIGFRR
                     GLLLGHSDGASIAAIYAGGVQDHRVRGVAMIAPHFIVEDMGLVSIAEARKAYEAGGLR
                     EKLARWHGDVDNTFYGWSGAWLDPKFRNWDISEYLAYIRVPVAILQGAEDQYGTIRQI
                     EIAQQECYCPVDVTIIPGAGHSPHREAPEATLDTIADFVGRILDADEGSRGQAA"
     gene            complement(3829..5367)
                     /locus_tag="VGA15_09665"
     CDS             complement(3829..5367)
                     /locus_tag="VGA15_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX9587999.1"
                     /translation="MSVYNAVSWLLDRNVDEGRGKKLAFTDTISELTYGELQQQSCRI
                     ANMLRRLGVRREERVAMIMLDTVDFPAVFLGAIRAGIVPVPLNTLLTSDQYAYVLADC
                     RARVLFISEALLPVVKDMLGRMQDLDHVIVAGKDAHGYKKLSDELASESDTFATAATH
                     ADEPAFWLYSSGSTGMPKGVRHLHSNLAATADTYAKQVLGIREDDVGLSAAKLFFAYG
                     LGNALTFPMSVGATTVLNSERPTPATMFALMNKYNPSIFFGVPTLFSAMLNDETTKHE
                     RCGSRLRICTSAGEALPESVGNNWKSRFGVDILDGVGSTELLHIFLSNAPGDIKYGCS
                     GRPVPGYKVRLVNEAGDDVPDGEVGELLVDAPSAGEGYWNQRSKSRQTFAGHWTRTGD
                     KYVRDADGRYTFCGRSDDMFKVSGIWVSPFEVESALITHPAVLEAAVVPEADPEGLLK
                     PKAFVVLRADADTDGLHEALKEHVKQKIGPWKYPRWIDVVDSLPKTATGKIQRFKLRD
                     GAAS"
BASE COUNT         1009 a         1754 c         1679 g         1070 t
ORIGIN      
        1 cggactttcg cgatatcgga gggatcgctg atccctgcgg ccttcatcgc ctcaacggtc
       61 cgctgcacca cgcggctgat gccggtctcg ccgacgaaca tgtgatgcgc ctcttcggtc
      121 agcatgaagc ggcaggtgcg cgacaacgga tcgaagccgg actgcgccag cgagtgcaac
      181 tgcatcttgc catcgcggtc agtaaaatag gtgaacatga agaacgacag ccagtccggc
      241 gtggcctcgt tgaaggcccc cagcatgcgt ggcgaatccg catcgcccga gcggcggcgc
      301 aagagttcat ccgcctcctc gcgcccgtca cgcccaaagt atttctgcag caggtagacc
      361 atcgcccaga gatggcggcc ttcctcgaca ttgacctgga acaggttgcg caggtcgtag
      421 agcgacggcg cggtcttgcc gagatgacgc tgctgttcga ccgatgccgg ttcggtgtcg
      481 ccctggatca cgatcagccg gcgcagcgtg gcgcggtgtt cgcccggtac ttcctgccat
      541 gccggcttgc cgaaattctc gccgaacggg atgacgcggt tttcttcctg cggcgccagc
      601 aaaatgcccc agcgataatc cggcatcttc acatagtcga acttggccca gccgcgcgga
      661 tcgaccgaat aggcggtgcg caagtaaacc agcgattgct gaaaaccttc cggccccatg
      721 tcgctccacc agtccatgta accgggatgc cagccttcca gcgccttgag cacctgccga
      781 tcctcgctga gattcacgtt gttgggaatc ttggtgctgt agtcgacgtt catgatgttc
      841 atggcgtgct ccttgttcgg tctcgtgtcc cggacgcggt gcagcgcgaa gcggtgcgcc
      901 gcagagccgg gacccattca gtggccgtca tgtgggtccc ggttctgcag cgcgtcacaa
      961 gcgtgccgcg cagcgcccgg gacacgaact cacacccgcg tcatatcgaa ctgcgccttc
     1021 tggccggtgc cgtaacggcg cagcgcgccg gtctcgccga ccgcgttggg acgctgaaag
     1081 atccaggtct gccacgccgt caggcgggag aagattttcg attccatggt ctcaggtccg
     1141 acgaagcgca ggttggcttc catgccggtc aggctgtcgg gcgagaagct ggtgcgctcc
     1201 tcgaaaaaca ccctgatctc gtagtcccag tcgatatcgt cgagcgcgaa ggtgacgagg
     1261 ccgagcgctt cggcttgctc cgcatcgagt gcctcaccaa tgctcttccg cgcgcgcaag
     1321 acatcttcag catcggcctg aaagcgcgat tgcagccgcg tcaggccgtg gctcatcggg
     1381 tacggcccga aattcatcgc ggacagctcg atggtcggcg gcgcgcggtt gtcgccctgc
     1441 ctcgaaccga tcagcatgta ggaccggtcc gccgcgaaca cgagttcggc cagcgtgccg
     1501 acaaagcacg agcccggctc gaccagcgtc accagcgtgc gcgaggtgac gtcgatgcgc
     1561 ttcagcaccc gcttccagta ttgcctgatt tcgttgacga gccagtgcgc cttgttggct
     1621 tccaggaatg cgtcgcaggc cagcacgcct gcgcgctcgc catgcgactt gaacaccagc
     1681 atcgcggttt cgagttcgtt gatgcgcaaa tgcaggattg cgtcgtccag ttcgcgcgcc
     1741 acctgcagcg gccagaacga cgcgccttgc gcggtcaagc cgtcgatatc ggcgggtggg
     1801 gctgcgtccg gcgcctgaat cgaaatggtc gcgatgcgcg cggcgcggtc gatatcgacg
     1861 ctgacatggc catagcggat gccgttgtcg tcgaagcggc gcgtcagcgg ggtcagcgtc
     1921 atgccttttc cggtgccgtt gcgcttcgag gccgcggcga attctgttgc acgttcggcg
     1981 actttggcct ccagtctgct gttgggcacg atctcgtcga cgaggcgcca ctgcaccgcg
     2041 cgcttgcctt tgatgccttc ctcgatggtg cagaaataat cggcgtggtc gcggcgcacc
     2101 ttgcgcttgt cgaccacgcg ggtcagtccg ccggtgcccg gcaataccgc cagcagcggc
     2161 acttccggca gcgcgaccgc cgccgagccg tcgtcggcga ggatgatgtg atcggtcgcc
     2221 agcgccagtt catagccgcc gccggccgcg gtgccgttga tgacggtgac aaaacgctgc
     2281 ccggaattct cggatgaatc ttccagcccg ttgcgggtct cgttggtgaa cttgcagaag
     2341 ttgaccttgt gggcgtgggt cgcccccgcc agcatgcgga tgttggcgcc ggcgcagaac
     2401 acccggttct tgcccgaccg caccacgacc accttcacgc cgggatgttc gaagcgcagc
     2461 cgctgcaccg cgtcggccag ctcgatgtcg acgccgaggt cgtaggaatt cagcttgagc
     2521 tgataacctt cgaacagccc gccgttctcg tcgacgtcca tggtcagcgt cgcgacctcg
     2581 ccgtcgacat ccagtttcca atgccggtag cgcgacggat cggtctgaaa atcgatgtgc
     2641 ttcgcgccgc ccgcgaggac gcgatcttca ccggccatgg gccacccttg agttcctatg
     2701 agttattttg atatgcagaa taatgcatgt tttgggagcg gtccagtcct gaaacgtcga
     2761 aatacggcat tttgtttcat gtccggaccg gccgactcta gccgcggttg acgtcctacg
     2821 cagcctgtcc cctcgagcct tcgtctgcat ccaaaatgcg tccgacaaaa tccgcgatcg
     2881 tatcgagcgt tgcctcaggc gcttcacgat gcggcgaatg tccggcgcct ggaatgatcg
     2941 ttacatcgac cgggcagtag cattcttgct gcgcgatctc gatctggcgg atcgtcccat
     3001 attggtcttc ggcgccctgc aggatcgcaa ccggcacccg gatgtaggcg agatattccg
     3061 aaatgtccca gttgcgaaat tttgggtcga gccaggcgcc gctccagcca tagaaggtgt
     3121 tgtcgacatc gccgtgccag cgcgccagct tttccctgag gcctccggcc tcataggctt
     3181 tccgggcctc cgcgatcgac accagtccca tatcttccac gatgaaatgc ggcgcgatca
     3241 tcgcgacgcc gcgcacgcgg tgatcttgaa caccacccgc atagatcgcc gcaatcgaag
     3301 cgccatcgga atggccgagc aagaggccgc ggcgaaagcc gatttggtcg agcagccttg
     3361 gcaaggtgtc gagcgcctcg atatgcatgt aatcgagcgg gcgcggcaac gcgaccgttg
     3421 tcgaggcgcc atagcccgct cgcgaataca ccaacacgcc ggcgccggtg gcggcggcaa
     3481 gccggtccgg aaaatctccc cacagcccgg cgcacccgag gccctcgtgc agcatgacga
     3541 tggtcggcgc gtccgcaggc gcgggaccga tcatgcggta ttcgaggtcg ctagtgccga
     3601 tggtgaggaa gcctgaagag tttaggcctt gcatgatatt tattttccaa agtgatccag
     3661 atcgcaatcc tcccgcgggt cgtcccggcg aacgccggga cccatagcca ccaacgttag
     3721 taattaacgg gagcgtctct ccgcactgtg ttcgatcatt aacgacacgg agtatgggtc
     3781 ccggcattcg ccgggacgac tcgcggagaa aatcttgctt gcttccttct atgacgccgc
     3841 cccatcgcgc aatttgaacc gctggatctt tccggtcgcg gtctttggca agctatcgac
     3901 gacatcgatc cagcgcggat atttccacgg gccgatcttc tgcttgacgt gctccttcag
     3961 cgcctcatgc aggccgtcgg tgtcggcgtc cgcgcgcagc accacgaatg ctttcggctt
     4021 cagcaagcct tccggatcgg cttcgggcac cacggcagct tccagcaccg cgggatgggt
     4081 gatcagcgcg ctctcgacct cgaacggcga cacccagatg ccggacacct tgaacatgtc
     4141 gtccgagcgg ccgcagaaag tgtagcgccc gtcggcgtcg cgaacatatt tgtcgccagt
     4201 gcgggtccag tggcccgcaa aggtctggcg gctcttgctg cgctggttcc agtagccttc
     4261 gccggcggag ggcgcatcga ccaggagttc gccgacctcg ccatcgggca catcgtcacc
     4321 ggcttcgttg accagccgca ccttgtagcc aggcaccgga cggcccgagc agccgtattt
     4381 gatatcgccc ggcgcattgg agagaaaaat atgcaacagc tcggtcgagc cgacgccgtc
     4441 gagaatgtcg acgccgaaac gtgacttcca gttgttgccg accgattccg gcagcgcctc
     4501 gcccgcggac gtgcagatgc gcaatcgtga gccgcagcgt tcgtgcttcg tggtctcgtc
     4561 gttcagcatc gccgaaaaca gcgtcggcac gccgaagaag atgctcggat tgtacttgtt
     4621 catcagcgcg aacatggtcg caggcgtcgg gcgttcggaa ttgagcacgg tggtggcgcc
     4681 gaccgacatc gggaacgtca gcgcgttgcc gaggccatag gcgaaaaaca gttttgccgc
     4741 tgacagcccg acatcgtcct cgcgaatgcc gagcacctgt ttggcatagg tgtccgccgt
     4801 cgccgccaga ttggaatgca gatgccgcac acccttgggc atgccggtcg agcctgacga
     4861 atacagccag aacgccggct cgtcggcatg ggtcgccgcc gtcgcgaacg tatcgctctc
     4921 gcttgcgagc tcatcggaca gtttcttgta accatgcgca tccttgccgg cgacgatgac
     4981 atgatcgagg tcctgcatgc gcccgagcat atccttgacc accggcagca gcgcctcgga
     5041 aataaacagc acccgggccc ggcaatccgc cagcacatag gcgtattgat ccgaggtcag
     5101 cagcgtattg agcggcaccg gcacgatacc ggcgcggatc gcgccgagaa acaccgctgg
     5161 aaaatcgacg gtgtcgagca tgatcatggc cacgcgctct tcgcggcgga caccgagacg
     5221 gcgcagcata ttggcgatgc ggcagctctg ttgctgcaat tcgccgtagg tcaattcgga
     5281 aatggtatcg gtgaaggcga gctttttgcc gcgcccttca tcgacattgc ggtcgagcag
     5341 ccagctcact gcgttgtaga cactcacgcg tcttctcccc gaattttaag aattataatt
     5401 catagaaagc caccgcacgc gcttgctgtc aatccttgcc ggcattatgt ttccggagcg
     5461 tgcggattcg cggccaaaaa ctgaccaggg gacatgaccg aagatcccga tc
//
LOCUS       DASWGM010000337        10614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1832626,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000337 DASWGM010000000
VERSION     DASWGM010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832626"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGA15_09670"
     CDS             <1..336
                     /locus_tag="VGA15_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588000.1"
                     /translation="DLATGWHQYRGDPAVSTTVWYRSGSPKGAPWTNQWGWQSDAVDK
                     LIDEAASELDTGKRKLLYADWVKVINEELPIWMVTERQFIAATSKQVQNHHTSPRWDS
                     GDWHDTWIS"
     gene            537..1508
                     /locus_tag="VGA15_09675"
     CDS             537..1508
                     /locus_tag="VGA15_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588001.1"
                     /translation="MRFFLFAIRRLAASVPTLLIVLAGLFMLLQFAPGDTVDALVAQM
                     GGGDSAMMEQLRKYYGLDATATVQLIRYLTRLVQLDLGFSAIYGKPVIDVILERLPAT
                     LLLMASSLSFAFAGGLVLGVLAARRVNQWPDGVISTIGAIFYATPAFWLGLMGIIIFA
                     VNLRLLPAGGLEEVGAGHTGLRRAVDIVWHLALPTATLGLIFLATYLRIMRASMLEVM
                     TLDFVRTARAKGLAKATVLRRHVLRNALLPMVTLVGLQAGTMLGGSVVVESVFALPGL
                     GRLAYEAVVQRDLNTLLGIVFVSALLVICVNFLVDILYARLDPRIAN"
     gene            1514..2344
                     /locus_tag="VGA15_09680"
     CDS             1514..2344
                     /locus_tag="VGA15_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588002.1"
                     /translation="MQTLTRFLGNPVALIGLSLLVLVAAMALSAASLFPNNPLDLAGR
                     PLQWPLSNPRFWLGTDVQGRDIAAVMFYGARVSLLIGVVATLIAMTIGILIGALAGYY
                     GGIADEMLMRLTEAFQTLPNFLLLLVLVAVFGSDIKTVTIAIGLVSWPASARLTRAEF
                     LSLRNREFVEACRTIGMRDMQIIFREILPNALPPVIVYASVVMAVAILLESALAFLNL
                     SDPNVPSWGNLIGGGRAVLRTQWYVSAIPGVAILMTVLAVSLIGQGLNDALNPRLKRR
                     "
     gene            2341..3978
                     /locus_tag="VGA15_09685"
     CDS             2341..3978
                     /locus_tag="VGA15_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588003.1"
                     /translation="MTGLLAISGLTVSLPPGADRPFALNDVSIDLRSGEIHCLIGESG
                     SGKSMIANAILALLPENVRITAGDIRFEDRSLLTLGEQGMRKIRGARIGMIFQEPMTA
                     LNPLRTIGDQIGEMFWTHTDLDSATIRTRVLSLLSDVQIAHPKEIERAYPHELSGGQR
                     QRAMIAMALALEPAILVADEPTTALDVTTQAQILALIRDLQARKGTSVLFITHDFGVV
                     AEIADRVTVLQHGRVVEHGARDQLLNHPSEPYTKELIAAVPSYLPPPSSAADGEPILT
                     ISHVSKTYRHFGLLRRSRRTTQALKDVSVSLTKGTTLGIVGESGSGKSTLARCIVRLI
                     DPDRGVIRLGDSELTSMSTRDLRAQSRHIQMVFQDPYGSLNPRRTAGELVTQGLILHG
                     MPGPQARAEAKQMFGVVGLDPQALDRYPHEFSGGQRQRIGLARALALRPDVLVADEPV
                     SALDVSVQAQILRLLANLRKTFGLSMIFITHDLRVAAQICDRVAVMKDGEIVEHGVTA
                     RVFASPEHDYTRLLLRSIPGQRWVPQAAGSYLAATAR"
     gene            complement(3996..4745)
                     /locus_tag="VGA15_09690"
     CDS             complement(3996..4745)
                     /locus_tag="VGA15_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9588004.1"
                     /translation="MAFALDHVVIAVDDLDRAVADYQSLGFTVYPGGVHHGGVSHNAL
                     IVFEDASYFEIIAYREAAPANRWWHVLTTAGEGIVDFAVLPENTEKDLQAARARGLNL
                     EGPTPGGRLRLDGVRLDWQIVRPTTTDLPFWCGDVTPRHLRVPEGAVRHHANGVIGIS
                     RVRILVSDAATSAERYRALVGPDAVGLEQGVARINLKKTVFELVGPDNDRARSHLDSR
                     GEGVLSLELCGSAVQALDRKLLHGADLAIAG"
     gene            complement(4801..5409)
                     /locus_tag="VGA15_09695"
     CDS             complement(4801..5409)
                     /locus_tag="VGA15_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase domain protein"
                     /protein_id="HEX9588005.1"
                     /translation="MSESIVVRASKDQPVTFTQEELGWLPWLDPLPEAEFTERHWAGL
                     VERSRARSDYFRLLVRDPQILEARTKTDKDIFYNIADGLPRAERELAAAATSRYNGCI
                     YCTSVHARFATVHSKRRDDIQRLLDHGVGADLGERWNAIVSASVALASTPIAFGQEHI
                     EQLRGVGLDDAEIVDVINGAAFFNWANRLMLSLGEPSTVVDA"
     gene            complement(5425..6060)
                     /locus_tag="VGA15_09700"
     CDS             complement(5425..6060)
                     /locus_tag="VGA15_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain protein"
                     /protein_id="HEX9588006.1"
                     /translation="MSTEPKDVIDLLAGIEAASSLDGIRARRLQARENAQKSYLALFE
                     PADIGDVAVAERYAVAAFVAGIHAEPAAASFYSAGFARSADRSELLDVLGAEIERAKT
                     EGPYGAFPAGLLSVEDKGGLIHRIAGKNRSVLGGRLAAALEHAHLLVFHPRDANPAAI
                     RVLLEAGWSNTAIVTLSQLVAFLCFQIRTIAGLRTLGASLGHGASVSAKAS"
     gene            complement(6100..7137)
                     /locus_tag="VGA15_09705"
     CDS             complement(6100..7137)
                     /locus_tag="VGA15_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative FMN-dependent luciferase-like
                     monooxygenase"
                     /protein_id="HEX9588007.1"
                     /translation="MKGSVSSKRLGFFTRLLDDAAPADRYRLAAAQIRHAECAGFDSA
                     WIAQHHFHESEGGLPAPFAFLGFVAAQTWRIRLGTGIVTLPLENPVRVAEDAAVLDLL
                     SNGRLELGVGTGGNPSAFAAFGLDSAERAEIFARNLDIVRNALAGGPLAGGDTLYPAR
                     PELCDRIWQATFSVGGGTRAGKAGDGLLLSRTQPRSRDAPRASLAEIQHPIIDAYLDA
                     LPPGREPRIMASRSVFAADDRQEAFRLADIGLRRARDHFVASGHASPGNALTDMIAAF
                     DVHVGTPDDVIASLHADSTLPRVTDLVFQAHSIDPPHRYILRSIELIAETVAPALGWT
                     KRTRADAALVE"
     gene            7656..9230
                     /locus_tag="VGA15_09710"
     CDS             7656..9230
                     /locus_tag="VGA15_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9588008.1"
                     /translation="MTRIVSTAAVRAALIEGREIALLDVREEGPYSRGHPLFAVSLPL
                     SRLELKVLDLIPRFDAPIVLYDDGGGLVERAEARLRALGYPDVARLDGDLDGWRRAGG
                     EVFIDVNVPSKAFGELVESKRHTPSLSAKEVDRLIAEKADVVVLDSRRFEEYRNMSIP
                     TGTSVPGGELVLRLHDLAPRPEMTVIVNCAGRTRSIIGTQSLINAGIPNPVFALRNGT
                     IGWTLAGLDLDRGAGRRFPKLSPAARAAAQSRASALSERAGVRSVSRDTVEAWRREPR
                     GFTLFLLDVRNPGEFEAGHLPGFRSAPGGQLVQATDEWVGVRHARLVLADDDGVRATL
                     AASWLIQMGWRNVHVLDGGITGGDLEIGPRPPRRPPFPATDVLLLAPEALAAELDETD
                     VMDLATSPRHTAGHVPGAWFVVRARLAEALPRLPAGRRLVLTSCDGTLARYAAAGLTD
                     RPVAVLAGGTRAWEAAGLPIERGLLRAADEVDDVYKRPYEGTDNAATAMQAYLDWEFG
                     LVEQLARDGTHGFQVF"
     gene            complement(10017..>10614)
                     /locus_tag="VGA15_09715"
     CDS             complement(10017..>10614)
                     /locus_tag="VGA15_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9588009.1"
                     /translation="YQLTSAQYTTMILNIMTALELDRPILIGCSIGGRIALHLALEHP
                     QEFRAIIGLQAGAHVDPYYDLNFLHRPDVHGGEVCAAIVSGLVGPDAPDAERWETLWH
                     YMQGGPGVFKGDLYFYKLDGDIRGRVAEIDTSRCPLFLLSGEYDYSCTPEETLSVASS
                     IQGCEVTIMKGLGHFPMSENPTEFLKHLLPVLEKIKGR"
BASE COUNT         1968 a         3351 c         3309 g         1986 t
ORIGIN      
        1 gatctggcca ccggctggca tcaatatcgc ggcgatcctg ccgtttccac cacggtctgg
       61 tatcggtcgg gctcgccgaa gggagcgccg tggaccaatc aatggggctg gcagtccgac
      121 gcggtcgaca agttgatcga cgaagctgcc tccgaacttg ataccgggaa acgcaagcta
      181 ttgtacgcag actgggtcaa ggtcatcaac gaagagctgc cgatctggat ggtcacggaa
      241 cgccaattta tcgcggccac cagcaagcaa gtgcaaaatc accatacctc accacgatgg
      301 gattccggcg actggcatga cacatggatc agttgaccgg atcagttgac ggcggccatc
      361 tgatcaggaa cgccgtttca ttctttttgg cgatggtccg gcgcgttttc aagcgaagtg
      421 gaaaccggtt cgcgtcaaga aaacgcgtca aatcaagaat ctagagcccc gtttcgattc
      481 tatcgaaacg gaaaaggctc tagaccaaaa cgtccacgga ggtcgggccg tcagccatgc
      541 gttttttcct gttcgcaatt cgtcggctcg cagcgagcgt gccaacgctg ctgattgtgc
      601 tggccggctt gttcatgctg ctccaattcg cgccgggcga cacggttgat gccctcgtgg
      661 cgcagatggg cggcggcgac agcgcgatga tggaacagct gcgcaagtat tatggcctgg
      721 acgcaacggc gacggttcag cttattcgct atttgacgag gttggttcaa ctggacctcg
      781 gattttccgc gatttacggc aagccggtga tcgacgtcat tctcgaacgt ctccccgcga
      841 cgctgctgtt gatggcatcg tcgctgagct tcgcgttcgc gggaggactt gtgctgggcg
      901 tgctggctgc gcggcgcgtc aatcaatggc cggacggggt catctcgacg atcggcgcga
      961 ttttctacgc gaccccggcg ttctggcttg ggctgatggg tatcatcatc tttgccgtca
     1021 atcttcgctt gttgccggcc ggcggcctgg aagaagtcgg cgccggtcac accggccttc
     1081 gccgcgccgt cgatattgtc tggcatcttg cgctgccgac ggcgacgctt ggcctgattt
     1141 tcctggcgac ataccttcgg atcatgcgtg cctcgatgct tgaggtgatg acactggatt
     1201 tcgtgcggac ggcgcgcgcc aagggactgg ccaaggcaac ggtgctgagg cgacatgtgc
     1261 tgcgcaacgc actgcttccg atggtcacgc tggtcggcct gcaggcgggc accatgctgg
     1321 gaggctccgt tgtcgtggaa agcgtctttg cgctgcctgg ccttggacgt ctggcctatg
     1381 aggccgtcgt gcagcgcgac ctcaatacgc tgcttggtat cgtgtttgtt tcggcgctgc
     1441 tcgtcatctg cgtcaacttc ctggtggaca ttctctacgc gcgtctggat ccgcgcatcg
     1501 cgaattgacc gcgatgcaga ccctcacccg attcttgggg aatcccgtgg cgctgatcgg
     1561 gctctccctg ctcgtgttgg tggccgccat ggcgctgagc gcggcaagcc tgtttccgaa
     1621 caatccgctg gatctcgcag gaaggccgct gcaatggcct ctcagcaatc cgcggttctg
     1681 gctcggcacc gatgtgcagg gccgcgacat cgcggcggtg atgttttacg gcgcgcgggt
     1741 ttccctgctg atcggcgtcg tcgcgacgct gatcgcgatg acgatcggca ttttgatcgg
     1801 agcgctggcc ggttactacg gcggaatagc cgacgaaatg ctgatgcgcc tgaccgaggc
     1861 ctttcagacg ctgccgaatt ttttgctgtt gctggtgctg gtcgcggttt ttggctccga
     1921 tatcaaaacc gtcacgatcg cgatcggatt ggtatcctgg ccggcctcgg cccggctgac
     1981 gcgggctgaa tttctctcac ttcgcaatcg cgaattcgtc gaggcgtgcc gcaccatcgg
     2041 catgcgcgat atgcagatca tctttcgcga gatcttgccg aacgcgctgc cgccggtgat
     2101 cgtctatgcc agtgtcgtca tggcggtcgc catcctgctc gagagcgcgc ttgccttcct
     2161 caatctctcg gacccgaacg tcccgtcctg gggtaacctg atcggcggcg ggcgggcggt
     2221 gctgcgcacc caatggtatg tatccgccat tccgggcgtc gcaatcctga tgaccgttct
     2281 ggcggtgtcg ctgatcggac agggattgaa cgatgcgctc aacccgcggc tgaagcgcag
     2341 atgaccggac tgcttgccat ttcaggcttg acggtttcgc ttccgcccgg agccgaccgg
     2401 ccgtttgcgc tcaacgatgt cagcatcgat ctgcgtagcg gggagatcca ctgcctgatc
     2461 ggcgaaagcg gatccggaaa gtcgatgatc gcgaatgcga tcctggctct gttgccggaa
     2521 aatgtccgga ttaccgccgg cgatatccgg ttcgaggaca gatcgctgct cacgctcggt
     2581 gagcagggca tgcgcaagat ccggggcgcg cgcatcggca tgatctttca ggaaccgatg
     2641 accgcgctta atccgttacg caccatcggc gatcagatcg gcgagatgtt ttggacccac
     2701 accgaccttg acagcgcgac gatccggacg cgcgtgctgt cattgctttc ggatgtccag
     2761 atcgcgcacc ccaaagaaat tgaacgcgcc tatccgcacg agctgtccgg cggccagcgg
     2821 caacgcgcga tgatcgcgat ggcgctcgcg ctcgaaccgg ccattctggt tgccgacgag
     2881 ccgaccacgg cgctcgatgt caccacgcag gcacagatcc tggccttgat cagggacctg
     2941 caggcgcgca aaggcacctc tgtcctgttc atcacccacg atttcggcgt cgtcgccgag
     3001 atcgcggatc gcgtgacggt gctgcagcat ggccgtgtcg tcgagcacgg cgccagggac
     3061 caattgctga atcatccgtc cgagccctac accaaggagc tgatcgccgc ggttccgtcg
     3121 tatttgccgc cgccttcgag tgcagccgat ggcgagccaa tcctgacgat ctcgcatgta
     3181 tccaagacct atcggcactt cgggctgctg cggcgcagtc gccgcaccac ccaggcactc
     3241 aaggacgtca gcgtgtcgct gaccaagggc accacactcg ggatcgtcgg cgaaagcggc
     3301 tccggaaaat cgacgctggc gcgctgcatc gtacgcctga ttgatccgga tcgtggcgtg
     3361 atccgcctcg gcgactccga gctgacatcg atgtccacgc gcgatctgcg tgcgcaatcg
     3421 cgccacatcc agatggtttt ccaggaccct tacggctccc tcaatccgag gcggaccgcc
     3481 ggcgaactgg tgacgcaggg attgatcctt catggcatgc ccggcccgca ggcacgggcg
     3541 gaagcaaagc aaatgtttgg cgtggtaggc ctcgatccgc aggccttgga tcgctatccg
     3601 catgaatttt ccggcggtca gcgtcagcgt atcgggcttg cgcgcgcgct ggcgctgcgt
     3661 cccgacgtgc tggtcgccga cgagccggtt tcggcgctcg atgtctccgt tcaggcgcaa
     3721 atcctccggc tgctggccaa tctgcggaag acgtttggcc tgtcgatgat cttcatcacc
     3781 cacgatttgc gcgtggcggc ccagatctgc gaccgtgtgg ccgtcatgaa ggacggcgaa
     3841 atcgtcgaac acggggtaac agccagggtg tttgcctcgc ccgaacatga ctacacgaga
     3901 ctgctgctcc gatcgattcc gggccaacgc tgggtgccgc aagcggccgg gtcatacctg
     3961 gcggcgaccg cgcggtgaag cgtcggctgc gacgctcacc cggcgatggc gaggtcggcg
     4021 ccatgcagca gcttccgatc gagcgcttgc accgctgaac cgcacagctc gagcgacaac
     4081 acgccctccc cgcgtgaatc gaggtgactg cgcgcacggt cgttgtcggg ccccaccaac
     4141 tcaaacaccg tctttttgag atttatccgg gcgacgccct gttccagacc gaccgcatcc
     4201 ggaccaacga gcgccctgta acgttcagcg ctcgtcgcgg cgtccgagac caaaatccgc
     4261 acccgcgaaa tgccgatgac cccgttggcg tgatggcgaa cggcgccctc ggggacgcgc
     4321 aaatggcgcg gcgtgacgtc gccgcaccag aacggcaggt cggtcgtggt tgggcgcacg
     4381 atctgccaat cgagccgcac accgtccagc cgaagccggc cgcctggcgt cggtccctcg
     4441 agattcaacc cccgcgcccg cgccgcctgg agatcttttt cggtgttttc cggcaagacc
     4501 gcgaagtcga cgatgccttc gccggctgtg gtcaggacat gccaccagcg gttggccggc
     4561 gccgcttccc gataggcaat gatctcaaaa tacgaggcgt cttcaaacac gatgagcgca
     4621 ttatgcgaca ccccgccatg gtggacgccg cccggataca ccgtgaaccc gagcgattga
     4681 tagtccgcga cggcgcgatc gaggtcgtca acagcaatga cgacatggtc gagagcgaaa
     4741 gccatgtttt gcattccgga tggcgggact agcggaaaaa cgacaatcgg gccgatgcgg
     4801 tcatgcgtcg actaccgttg agggctcccc gagggagagc atcagcctgt tggcccaatt
     4861 gaagaatgcc gcaccgttga tcacgtcgac gatttcggcg tcatcgagac ccacgccgcg
     4921 caattgttca atatgctctt gaccaaaggc gatcggcgtc gaggcaagcg caaccgaggc
     4981 gctgacaatc gcgttccagc gctcgccgag atcagcaccc acgccgtggt cgagcaggcg
     5041 ctgaatgtca tcgcggcgct tggagtggac ggtggcgaaa cgcgcatgca cggacgtaca
     5101 atagatgcat ccattgtagc gggacgttgc cgcggccgcg agttcgcgct ccgcccgcgg
     5161 caagccgtcc gcgatgttgt agaagatgtc cttgtcggtc ttggtcctgg cttcgagaat
     5221 ctggggatcg cgcaccagga gccgaaagta atccgacctt gcgcgcgatc gctcgaccaa
     5281 tccggcccaa tgccgctcgg taaactcggc ttcgggaagc ggatcaagcc agggcagcca
     5341 gcccaactcc tcctgggtga aggtcacggg ctgatccttg ctggcgcgaa cgacgattga
     5401 ctccgacatg atcctgtcgc cccctcaact ggctttggct gaaacgcttg cgccgtgccc
     5461 gagtgacgcg ccaagcgtgc ggagacccgc gatcgttcga atctgaaaac acaggaacgc
     5521 aacgagttgc gacaacgtca cgatcgccgt gtttgaccaa ccggcttcca gcagcacccg
     5581 gatggccgcc ggattggcat cacggggatg gaagaccagc aaatgtgcgt gctcaagggc
     5641 cgcggctaat cgtccgccaa ggacactccg gttttttccg gcaatgcgat ggatcagacc
     5701 ccccttgtcc tcgaccgaga gcagcccagc gggaaaagcg ccgtacggac cttccgtctt
     5761 tgctcgctcg atttcggctc cgagcacatc gagcagttcc gatcgatctg cggatcttgc
     5821 aaacccggcc gagtagaagc tggcggcggc cggctcggca tggatgccag ccacgaaagc
     5881 cgcaacagca tatcgttccg cgaccgcgac atcgccaata tcggcgggct cgaacaaggc
     5941 aagatagctc ttttgagcat tctcccgcgc ctgcaagcgc cgggcgcgga tcccgtccag
     6001 gctggatgcg gcttcgatac cggcgaggag atcgatgaca tcttttggct ctgtactcat
     6061 agctgtactc atagttcggc ttctttctcc cgcgtcttgt tactcaacca gcgcagcatc
     6121 ggcccgcgtc cgttttgtcc agcccaatgc gggcgcaacc gtttcggcga tcaactcgat
     6181 ggaccgcaga atatatcgat gcggcgggtc gatcgaatgc gcctgaaaga cgaggtcggt
     6241 cacccgcggc agcgtgctgt cggcatgcag cgacgcgatg acatcatccg gggttcccac
     6301 atgaacgtcg aacgcagcga tcatatcggt cagcgcgttt cccggcgatg cgtgtccgga
     6361 agctacgaaa tgatcgcggg cgcgccgcag tccgatgtcg gccaggcgga atgcttcctg
     6421 acgatcgtcg gcggcaaaga cgctgcgcga cgccatgatt cgcggctcgc gtcccggcgg
     6481 cagcgcgtcc aggtaagcgt caataatggg atgctgaatc tcggcgagcg acgcccgcgg
     6541 ggcgtctctg gatcgcggct gggtccgaga caacagcaag ccgtcgcccg ccttgccggc
     6601 tcgcgttccg ccacccactg aaaaggttgc ttgccagatc cggtcgcaca attccggccg
     6661 cgctggatag agcgtatcgc caccggcgag cggcccacca gccagggcgt tgcggacgat
     6721 atcaagattg cgcgcgaaaa tttccgcgcg ctccgcgctg tccaagccaa aggctgcgaa
     6781 agccgacggg tttccgccgg tcccgacgcc gagttcgaga cggccgttcg acagcaaatc
     6841 gagcacagcg gcgtcctccg ccaccctcac gggattttcc agcggcagcg tgacgatgcc
     6901 ggtcccgagg cgaatgcgcc aggtctgtgc agcaacgaaa ccgagaaagg cgaaaggagc
     6961 aggaagaccg ccctcgctct cgtgaaaatg gtgctgagca atccatgcgg aatcaaagcc
     7021 tgcgcattcg gcgtgcctga tctgcgctgc agccaggcga tatcggtccg ctggagcggc
     7081 atcatcgaga agccgcgtga agaagcccag tctcttcgaa cttaccgaac ccttcatcga
     7141 acccttcgaa ccggaacaac ggaagatgca ggcccagccg ccttttgccc gtcgtgtgaa
     7201 acgcgaagtc cgacgatcca ctggtcccgc gcatcgacca caggacgatc aggcaagaga
     7261 ttatctgaac caaggcaacc tcccaagtta tggttctgac gctttttaca aggtgtcaac
     7321 gagaatgttc tctcacgggt ccagtcgcct gcagcaagtt tgttcttaag aacgatcacg
     7381 atcggcgcac tgtgataact ctgtggccgc gctcgaatcg agccatgcga acgccgttaa
     7441 ttgaacacgg ttgcgcaaaa caaaactggt tggcccgtcg tcttcatgcg aaaatacaga
     7501 catcgccgaa gtggcgatcc agagaacgcc cttccatatc catttcgtgc caagcatgaa
     7561 ctccctacac tcgccgtcgc aatctgtagg atcgctccga gggctgagcc ggcccactgg
     7621 tgcgagcccg ccagcgccca ccaggaagat ccgtcatgac ccgcattgtc tccaccgcgg
     7681 ccgttcgcgc cgccctgatc gaagggcgcg agattgcgtt gctcgacgtg cgcgaagaag
     7741 ggccctactc gcgcggtcat ccgctgttcg cggtgtcgct gccgctcagc cggttggagc
     7801 tgaaagtgct cgatctcatc ccgcgcttcg atgcacccat cgttctctac gatgacggcg
     7861 gcggactggt ggagcgcgcc gaggcccgtc tgcgcgcgct cggctacccc gacgtcgcgc
     7921 ggctcgacgg cgatctggac ggatggcggc gcgccggcgg cgaggtgttc atcgacgtca
     7981 acgtgccgag caaggccttt ggcgaattgg tcgagtccaa acgccatacg ccatcgctct
     8041 ccgccaagga agtcgatcgg ctgatcgccg agaaggccga cgtcgtcgtg ctcgattccc
     8101 gtcgtttcga ggaatatcgc aatatgtcga ttcccaccgg caccagcgtg cccggcggcg
     8161 aactggtgtt gcgcctgcac gacctggcgc ctcggccgga gatgacggtg atcgtcaact
     8221 gcgccgggcg gacccgctcg atcatcggca cccagtcatt gatcaatgcg gggattccca
     8281 acccggtttt cgccctgcgc aacggcacca tcggctggac gctcgccgga ctggacctcg
     8341 atcgcggcgc cggccggcgg tttccaaagc tctcgccggc ggcacgcgcg gcagcgcaaa
     8401 gccgggccag cgcgctttcg gaacgagcgg gggttcgctc ggtcagtcga gatacggtcg
     8461 aagcatggcg gcgggagccg agggggttca cgctgttcct gctcgacgta cgcaatcccg
     8521 gggaattcga agccggtcat ctgccgggct tccgctcggc accgggtggg caattggtgc
     8581 aggcgaccga cgaatgggtg ggggtacgcc acgcccgcct cgtcctggcc gacgacgacg
     8641 gagtgcgcgc aacgctggcc gcctcctggt tgatccagat gggatggcga aacgtccacg
     8701 ttctggacgg cggcatcacc ggcggcgacc tcgaaatcgg cccgcgcccg cctcgccgcc
     8761 cgcccttccc ggcgactgac gtgttgctgc tcgcgcccga ggcgctggcg gcggagctcg
     8821 atgagactga cgtgatggat ctggccacca gcccgcgcca taccgccggg cacgtgcctg
     8881 gcgcctggtt cgtcgttcgc gcgcggctcg ccgaggcgct gccccgattg cccgccggcc
     8941 gccggctggt gttgacctcc tgcgacggca ccctggcgcg ttacgccgct gccggcctca
     9001 ccgatcgacc ggtcgccgtt ctggccggag gaacccgggc gtgggaggcg gccggattgc
     9061 cgatcgagcg cggtctgctt cgcgccgccg acgaagtcga cgacgtctac aagcggccct
     9121 acgagggcac cgacaacgcg gcgaccgcga tgcaggccta tctcgattgg gaattcggcc
     9181 tcgtcgagca gctcgctcgc gacggaaccc acggctttca ggtgttctga gcaaaagggc
     9241 gaggggggcc ggtcgacgtc gcgcgttccc aaaccgaagg tcgggagttc aattctctcg
     9301 aaaccggcac gctcgaagcc gagcagctcg ctgcatgtct cacgaccatc gccgtcgcgg
     9361 acaagactga agccacgtcg ttgaagtcca agtcgtcgaa gtccaagtcg tcgaagtcga
     9421 aatgagccca cgttatccac agcgttcagc gaccgcacac gtcacgtcgc ttgcttccgc
     9481 actgcgttct cggtgaagtg tttgtgggcg aggtgtgtga tggaatgggt aagagagcgc
     9541 gaacttttga tcgcgcaaac attggcgttc gttcaatcgg tctccgacag ggaacgagac
     9601 gacaagttgc gagacgccag gaaactggac gccagaaaac tggacgcatg gaaactagac
     9661 gcgaggaaat caggcgcgga gcctgataga atgccttgcg ttgaggccgc gccgatcgac
     9721 gcgatcaagt tcgtcgaacc gccggccagc accccattca acatccaggc cccgcggcca
     9781 acagtctccc gcgaagtccg aaccgaaata cagaaccggg tggcaagctt tcgcgccaac
     9841 cagcagcggt tccatcggga gcgggaagcg tattgccgcg ccacgttggc caggatgcgc
     9901 gtcgccatcg ggactgattc cgcgccccct ccactgcgga aataaaccag cctcaacggg
     9961 cacgcacatg aaggccgcca ggccccgctt cgggcgctgc gccgccggcg caaaaatcac
    10021 cggcctttga tcttctccag caccggcagc aggtgtttta gaaactccgt cgggttctcg
    10081 ctcatcggaa aatgcccgag gcccttcatg atggtgactt cgcacccctg gatgctgctt
    10141 gctacgctga gcgtttcctc cggcgtgcag gaatagtcgt attcgcccga cagcagaaac
    10201 agcggacagc ggctggtgtc gatctccgcg acgcggcctc ttatgtcgcc gtcgagcttg
    10261 tagaaataca gatcgccttt gaagacgccc gggccaccct gcatgtagtg ccacagcgtc
    10321 tcccagcgct ccgcatcggg cgcgtcgggc ccgaccagtc cggaaacgat ggcagcgcag
    10381 acttcgccgc catggacgtc ggggcggtgc aggaaattca gatcgtaata gggatcgaca
    10441 tgggcgccgg cctgcaagcc gatgatggcg cggaactctt gcggatgttc gagcgccaga
    10501 tgcagcgcga tgcggccgcc gatcgagcat ccgatcagga tcggccggtc gagttcgagc
    10561 gcggtcatga tgttgaggat catcgtcgtg tattgcgccg aggtgagctg atat
//
LOCUS       DASWGM010000338         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_864848,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000338 DASWGM010000000
VERSION     DASWGM010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864848"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VGA15_09720"
     CDS             <1..1299
                     /locus_tag="VGA15_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HEX9588010.1"
                     /translation="ELPRELNGTLYRNGPNPQFDAPGAHWFVGDGMLHAFHLENGRAS
                     YRNRWVRTPKWQAEHDAGRALFGGFGRKLPDAPASTTQDGGVANTNIIFHAGRLLALE
                     EGHLPTEIEPGTLNRRGYCDYDGSIAGPFTAHPKIDPVSGEMVFFGYNAAGPLTPALS
                     FGSVNASGVVTRFDRLTAPYASYVHDFIVTEHHLLFPILPITGSMERAMRSQPAYAWE
                     PDKGAYVGVMKRNGPAGDIVWFRAQNCYVFHVMNAWEDGTRIIADVMQFEEPPLFTHP
                     NGLPTDPKKSRARLCRWIFDLAGNTDRFTQTYLDDVTGEFPRIDDRRAGRASAHGWYA
                     CANPDLTGIGTLCGVVHVDGKGSRRGQYRLPAGDTISEPVFVPRGDEAAEGDGWPLAS
                     VWRAGENRSDLAVFNATDVEAGPVALVQLGHRVPDGFHGN"
     gene            1480..2052
                     /locus_tag="VGA15_09725"
     CDS             1480..2052
                     /locus_tag="VGA15_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588011.1"
                     /translation="MTIFLILAPFGAFTMLMLVTSATVSLFAAAAVGLAVIAFDIVRG
                     RAIKILGAGSVILFAALGFYLTLVDPGLSNSAVKLTVDAGVLAISLMSMAIRRPFTLQ
                     YALEVVDADTAKLPGFMKANYVITGAWTGAFLLMMIANALMIYVPGLPLWSGLLIAFA
                     ARNSAIYFTKWYPQYRRAKFGPPAGALSRS"
     gene            2095..>2612
                     /locus_tag="VGA15_09730"
     CDS             2095..>2612
                     /locus_tag="VGA15_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEX9588012.1"
                     /translation="MKDVFARLASDFFSTIVFLALYLTTGDVLLATGVAIAGAVGQVI
                     YARVKGQQLGFMTYASLALVIVLGSATLLTNDPRFVLAKPAVGHFAIGAIMLKRGWML
                     RYMPPIVSETIPEYITVAGYAWAALMFALGLGTIAVASTGDLKLWALYVSVVLVGAKV
                     AAFAIQYVAFRLL"
BASE COUNT          435 a          881 c          773 g          523 t
ORIGIN      
        1 gagttgccac gcgaactcaa cggcacgctc tatcgcaatg gccccaatcc gcagttcgac
       61 gcgccgggcg cgcactggtt tgtcggcgac ggcatgctgc acgctttcca tctcgagaac
      121 ggccgcgcca gttatcgcaa ccgctgggtc cgcaccccga aatggcaggc cgagcatgac
      181 gccggccgcg ccctgttcgg cggttttggg cgcaagctgc ccgacgcacc agcctcgacc
      241 actcaagacg gcggcgtcgc caacaccaac atcatttttc atgccgggcg cctgctcgcg
      301 ctcgaggaag gccacctgcc gaccgagatc gagccgggca cgctcaacag gcgcggctat
      361 tgcgactatg acggcagcat cgccggaccg ttcacggcgc atcccaaaat cgatccggtc
      421 agcggcgaga tggtgttctt cggctacaat gccgccggac cgctgacccc tgccctttct
      481 ttcggctcgg tcaacgcatc cggcgtggtg acgcggttcg atcgcctcac agcgccctat
      541 gccagctatg tgcacgactt catcgttacc gaacatcatc tgctgtttcc gattctcccg
      601 atcaccggca gcatggaacg cgcgatgcgc agccagccgg cctacgcctg ggagccggac
      661 aagggcgcct atgtcggcgt catgaagcgc aatgggccgg ccggcgacat cgtctggttt
      721 cgcgcccaaa actgctacgt gttccacgtc atgaacgcct gggaagacgg cacgcgcatc
      781 atcgccgacg tcatgcagtt cgaagagccg ccattgttca cccatcccaa tggtttgccc
      841 accgatccaa agaagtcgcg ggcacggctg tgccgctgga tcttcgatct cgccggcaat
      901 accgaccgtt tcacgcaaac ctatctcgac gatgtcaccg gcgaattccc gcgcatcgac
      961 gatcgccgcg ccggtcgtgc cagcgcccac ggctggtacg cctgcgccaa tcccgacctg
     1021 acggggatcg gcacgttgtg cggcgtggtg catgtcgacg gcaagggctc gcgccgcggc
     1081 cagtaccggc tgccggcggg cgataccatc tccgagccgg tgtttgtccc ccgcggcgat
     1141 gaggccgccg agggcgatgg ctggccgctg gcgtcggtct ggcgcgccgg cgaaaaccgc
     1201 agcgatcttg cggtgttcaa cgcgaccgac gtcgaggccg gcccggtggc gctggtgcag
     1261 ctcgggcacc gggtgcccga cggctttcac ggcaactgaa gcctcgaacc cggaatccag
     1321 agatgatcag ctccagattc cgggttcgcg cttcgcgcgc cccggaatga cgttgaacat
     1381 cactctattt tgacactgta aagatttcgc ttgacctttc cttctttggt ctctacttcc
     1441 atctttacga tgtaaagatt aaccgcccgc gaggctgcca tgacgatttt tctgatcctt
     1501 gcccccttcg gtgcctttac gatgctgatg ctggtgacct ccgccaccgt aagcctgttc
     1561 gcggcggccg ccgtcggcct cgccgtcatc gccttcgata tcgttcgcgg ccgcgcgatc
     1621 aagatactcg gtgcgggttc ggtcatcctg ttcgccgcac tcggcttcta cctcactttg
     1681 gtcgatcccg gcctgagcaa ttcagccgtg aagctgacgg tcgacgccgg cgtgctcgcg
     1741 atctcgctca tgtcgatggc gatccgccgg ccgttcacgc tgcaatatgc gcttgaggtg
     1801 gtcgacgctg acacggcaaa actgcccggc ttcatgaagg caaactacgt catcaccggg
     1861 gcctggaccg gcgcgtttct gctgatgatg atcgccaacg ccctgatgat ctatgtgccg
     1921 ggcctgccgc tgtggtccgg actcctgatc gcattcgccg cccgcaacag cgcgatctat
     1981 ttcaccaaat ggtatccgca ataccggcgg gcaaaattcg gcccgcccgc cggcgcacta
     2041 tcccgttcct gattttcgca agtaggattt aacgataccc agcggagcgc gacgatgaag
     2101 gacgtatttg ccagactcgc cagcgacttt ttctccacca tcgtgttcct ggcgctgtac
     2161 ctcacgacgg gcgacgtgct gctggcgaca ggtgtcgcga ttgccggcgc ggtgggccag
     2221 gtgatctatg cccgcgtcaa gggccagcaa ctcggcttca tgacctatgc cagcctggcg
     2281 ctggtgatcg tgctcggcag cgcgacgctg ctgaccaacg atccacgttt cgtgctggcc
     2341 aagccggccg tcgggcattt cgccatcggc gccatcatgc tcaagcgcgg ctggatgctg
     2401 cgctacatgc cgccgatcgt gtcggaaacc attccggaat acatcaccgt tgcgggctac
     2461 gcctgggcag cgctgatgtt cgcgctcggc ctcggcacca tcgcggtggc ctcgaccggc
     2521 gatctcaagc tatgggcgct ctatgtgtcg gtggtcctgg tcggcgccaa ggtcgcggcc
     2581 ttcgccattc aatatgtcgc gtttcgcctt ct
//
LOCUS       DASWGM010000339        15563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2529741,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000339 DASWGM010000000
VERSION     DASWGM010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15563
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2529741"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..647
                     /locus_tag="VGA15_09735"
     CDS             105..647
                     /locus_tag="VGA15_09735"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9588013.1"
                     /translation="MIAHGFALFDTAIGRCAIAWSGRGILAVQLPEADERKTRRRLIR
                     RFPQAHEAPPPPAVARALEGIAALLRGERIDLSAVALDLDGVPPFHRRVYEVTRTIAP
                     GVTRSYGEIAARLGAPRSARAVGQALGRNPFAILVPCHRVLAAGGKPGGFSANGGVAT
                     KLRLLAIETAQNNGAPALPL"
     gene            complement(905..1276)
                     /locus_tag="VGA15_09740"
     CDS             complement(905..1276)
                     /locus_tag="VGA15_09740"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEX9588014.1"
                     /translation="MKFKLDENIGRRGLEFLTSSGHDVKTARGQELGGATDEELFKAC
                     AVEGRALVTLDRDFGQVLRFPPEKTAGVVILEVGPHATLRAILDRLRDLLAVLETRPL
                     AGALWIVEPGRLRIHIGDHEE"
     gene            complement(1273..1515)
                     /locus_tag="VGA15_09745"
     CDS             complement(1273..1515)
                     /locus_tag="VGA15_09745"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX9588015.1"
                     /translation="MNRLLERISIDPEICGGRPCIKDTRIWVSLILDFLASGMTESQL
                     LAEYPQLVHEDVLAAIAYGAEASRERVVPVPIGGAA"
     gene            1765..1998
                     /locus_tag="VGA15_09750"
     CDS             1765..1998
                     /locus_tag="VGA15_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588016.1"
                     /translation="MIKNDFHTAQYEPFLNDATAALFKLTHCPSANRASSLSVLRYCV
                     GTLGSFAWGEVSADDGATWRLAVEFFARRVDAA"
     gene            2062..2703
                     /locus_tag="VGA15_09755"
     CDS             2062..2703
                     /locus_tag="VGA15_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9588017.1"
                     /translation="MALAFYYASGSPYAWRVWLALEHKGIPYERKTLSFDAGDLKTPE
                     FAALNPRRKVPVIVDEGFALYESGAIVEYLEDKRPGEPRLFSADLRQRALQRRMVREA
                     DQYFAAPMERLVEAVLFTPPERWSEERIAAARADAHRELAIWETAIAGEYLAGALSAA
                     DFTLYPYVALVLRMGKRRPDLVTVDLIGPSISAWMRRMEALPVTRKTWPPHWK"
     gene            3042..4040
                     /locus_tag="VGA15_09760"
     CDS             3042..4040
                     /locus_tag="VGA15_09760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9588018.1"
                     /translation="MYGALPDLITITVADVILFGSYAVTWTGARIFDGRGPRPGSLIT
                     GATVWLLACQFAEFAQAPAIRASLSAAIIAAFMWLTAYEFWRSRAERLLSRWPAIFIL
                     FANGAVFLLRSPPIARLAWPTDEQVFSSAWLTVISSESLLATISTAFILLAMAKERAE
                     LRHKTAATKDPLTGLANRRSFFQEASRLMQTSDRPVALLMVDLDHFKSINDQFGHAAG
                     DEILRIFAKTASANLRPSDLVGRLGGEEFAVLLPAASQEGAWFVAERLRSVFAAAAGT
                     VNGLSIQATASVGISILDPGQDMSALLDRADQALYRAKASGRNRVAGPQPQFSPAA"
     gene            complement(4121..4633)
                     /locus_tag="VGA15_09765"
     CDS             complement(4121..4633)
                     /locus_tag="VGA15_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588019.1"
                     /translation="MDVADGERFFTKIAPLATAMVRFQSNGGSVVTGIQMGEMIRLKQ
                     FHTLVPAGARCASACALAWLGGARRFMGPGARIGLHAASDPKSGQVTGVGNALLGAYL
                     NRVGLSYSAVIYVAQARPDSVTWLSFADAKRLGIEVTLLKSAAGKRDPPVQTRVGAGV
                     SDGLSGPALR"
     gene            4943..5188
                     /locus_tag="VGA15_09770"
     CDS             4943..5188
                     /locus_tag="VGA15_09770"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9588020.1"
                     /translation="MLKERRRHARHSINRVAKVQSAGSLPRDCLVTDISDAGARLYAE
                     GIVIPDQFVLLITGSQTVRRQCRVVWRLGNEIGVEFA"
     gene            5368..6093
                     /locus_tag="VGA15_09775"
     CDS             5368..6093
                     /locus_tag="VGA15_09775"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX9588021.1"
                     /translation="MPNIALDWTTVDWANVGLLSAIAFLAALLGNILIFKHRLWAAIL
                     AGLLFAAAYVFLAYYPHGLTVPGLKTGARKAAVVVGLNLTSPEIAPGATIQNEQVYRG
                     FDCNGTNISPELRWSGAPSTAKSFTVTLYDPDAPTGSGWWHWVIVNIAPTTTFLPRNA
                     GDPKMGLAPPGTIQIRTDFGTVGYGGPCPPQGDRPHRYVFTIFAVDVDKLPVDESASA
                     ALVGFNLHSHTLTKATLTGFYRR"
     gene            complement(6242..7075)
                     /locus_tag="VGA15_09780"
     CDS             complement(6242..7075)
                     /locus_tag="VGA15_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX9588022.1"
                     /translation="MAEGSLEILLRRDRVVVAGALAVLTALAWAYVLWLAFGMRMPAT
                     EANGMPMGDMGAMLAPSPRPWSVAEFLFVLTMWAVMIVGMMTPAAAPMILIYARVARQ
                     AALQGHPFAPTGWFASGYLLCWIGFALIATSAQWALERAALRAPMSGAAGSALGGLVL
                     LAAGIYQLTPLKTACLSQCQSPLFFIHRHGGFRADAPGSFRLGLAHGAYCVGCCWALM
                     TLLFVGGVMNVLWVVGLTALVLLEKLSPPGRTLPVLVGCALSAGGVWLVATSGLVPSF
                     R"
     gene            complement(7081..7716)
                     /locus_tag="VGA15_09785"
     CDS             complement(7081..7716)
                     /locus_tag="VGA15_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX9588023.1"
                     /translation="MPDASWMIKGREFGHCNCAYGCPCQFNALPTYGDCQAVVGMQID
                     EGYHGPTRLDGLRTVSILKWPGPIHEGKGEASIIIDKRATAAQREALLRILGGQDTVP
                     GATMFQVFSTTFDKVHDPIFADIEFEVDVDARKARLKVADLVEARGEPIRNPVTGNEH
                     RARFDMPNGFEFAAAEVGRGWAKTNGPIRLELADSHAHFADLHLCQTGVIR"
     gene            7912..8742
                     /locus_tag="VGA15_09790"
     CDS             7912..8742
                     /locus_tag="VGA15_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9588024.1"
                     /translation="MKRQCPALVALAGLLVASVALAQERSITVASTTSTEQSGLFGYL
                     LPQFTAKSGIRVNVVAVGTGQALDIGRRGDADVVFVHDKAAEEKFLAEGFGVKRFDVM
                     YNDFVVIGPGSDPARIAGGEDVLEALRKIAAIKAPFISRGDRSGTHAAELRYWHDAGI
                     DIGAVKGAWYREIGQGMGAALNMAAAANAYLLCDRGTWLSFQNRGELSILVAGDRRLF
                     NQYGVMLVNPAKHPNVRTREGQAFVDWLVSPEGQRTIAAYKVGGEQLFFPDAGDLKGS
                     "
     gene            complement(8775..8927)
                     /locus_tag="VGA15_09795"
                     /pseudo
     CDS             complement(8775..8927)
                     /locus_tag="VGA15_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9373..10152
                     /locus_tag="VGA15_09800"
     CDS             9373..10152
                     /locus_tag="VGA15_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9588025.1"
                     /translation="MLDRRTVAAALLAGAATSIIGAGKARAQTQPLTPARNVVLVHGL
                     YADGSCWLRVIPLLQQVGLNVTAVQNPLRSLDEDCEFTRRTLTLQDGPTVLVAHSYGG
                     EVVTQTGGDPKVSALVYCSARAPDANEDYTALAKAYPTPPASAGLVHANGYVQLSEEA
                     FLRDFAGDIDPGTARALYAVQGRAAEPIFGDRVTQAAWRTKPSFYQVSSKDRTINPDL
                     QRFMAKRMKAKTIEIESSHLGIISHPRKIANLILEAAGHAT"
     gene            complement(10442..10897)
                     /locus_tag="VGA15_09805"
     CDS             complement(10442..10897)
                     /locus_tag="VGA15_09805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX9588026.1"
                     /translation="MNRFTIIALSAVMLAYAPGPSLAAPADEVNVLIGRWAEAFNSND
                     AQALAKLYAQDATLLGTLSPTLAENSESVQKYFSFLPGSSAKVSVGELRIIAISDTSA
                     LATGFYDFAETQDGKPLEVPARFTFVAIKRGNDWLIIHHHSSRRPEPPK"
     gene            complement(11026..12537)
                     /locus_tag="VGA15_09810"
     CDS             complement(11026..12537)
                     /locus_tag="VGA15_09810"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9588027.1"
                     /translation="MAPISDARRDVCCACGSSRTLSRRGFLCTGVAAAAAVPALVAST
                     VASAQAQPSRAPGSDRPILIRNGCVLSLDPTVGDFERADVLISGSKIAAVGPDLSAPD
                     AEVIDAGRAIVMPGFIDTHRHMWEGILRNMLPDASLDDYLKIVQLQLGPHYRPEDVYA
                     ANLVSALGAIEVGITTILDWSHIQNSPEHTDAAIEALAHSGIRAVFAYGIPSNGLPDF
                     RDDPRQKYPDDIRRLRKQCFSSSDQLLTLALASPSNPPDRVAEAWAVAREVGAPITIH
                     VGGGNAITGVVQKLGDKGVLKADTTYIHCCTLSDTEFKMIADSGGTFSLAGYVEMIMG
                     DGTPPVQKVLDLGLRPSLSIDVETTAPGDLFTQMRTILALQHNNLWIRGLAGDKNLPK
                     LLTARDVIEFATVEGARANHLDRKVGTLTPGKEADLILLRTDRFSVMPMNSATGAIVA
                     SMNASNVDTVLIAGKVMKRNNQMVGVDYHKMVNLVEASRDYIMRNANYERAKL"
     gene            complement(13087..14226)
                     /locus_tag="VGA15_09815"
     CDS             complement(13087..14226)
                     /locus_tag="VGA15_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX9588028.1"
                     /translation="MSVTLPPKGRDLRLDFFRGVANWWIFLDHIPNNIVNWITPRNYG
                     FSDAADLFVFISGYTASFVFARVMLERGFLIGATRLGRRAWQIYVAHVLLFVIYVAEI
                     GWLATRYDNPVLAQKFNVFGFIRDPFTTLYEGLILKFKPVNMDVLPLYIVLMACFAPV
                     LWMMLRRLNLVLAISALLYFAARHFDWNLPSYPIGVWYFNPFCWQLLFVFGAWFALGG
                     TSQSMPLIRSKALPALGCAYLLFALVMTMAGRFPELRELLPAWLYETFNPNDKTNLAP
                     YRFLHFVVIAFLIVRFVPRDWRALERPVAWPLIVCGQQSLEVFCVGIFLSSLAYFVLV
                     EVSNEIWMQVLVSLVGVALMTGVAAYRSWSKKVEKTKASPAPTPV"
     gene            14216..14488
                     /locus_tag="VGA15_09820"
     CDS             14216..14488
                     /locus_tag="VGA15_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588029.1"
                     /translation="MTDIGALPDDGGYELAARRRGLHHYFLYPEVAAHVMRVTETAHW
                     LEWQDWADPILQKPYDIKNGLLHIPEVPRVSLEWDEDAMAANQDRL"
     gene            14925..15344
                     /locus_tag="VGA15_09825"
     CDS             14925..15344
                     /locus_tag="VGA15_09825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9588030.1"
                     /translation="MDKNSFLWKMVDGRLPLSPCDKTLGFEFVQIDGKRGTVEIKFEA
                     KPEFLNPAGNVQGGFLAAMLDDTMGPALAAMLDAGEFAPTLNLNVQFHRPAKVGPLRG
                     VGRVVLRGKEICQLSSELFQNDKIVATATATAVIRKM"
BASE COUNT         2915 a         4954 c         4884 g         2810 t
ORIGIN      
        1 cccactaccc gatggtacca taaggggcat gcggcggcac cgcggtgccg gaatcccggg
       61 agcaagatta agtgcggcgc gcgcggcggg gctaggatcc cggcatgatt gcccatggat
      121 ttgcgttgtt cgatacggcg atcggccggt gcgccatcgc ttggagcggg cgcggcatcc
      181 tcgccgtgca gcttccggag gcggacgagc ggaaaacgcg caggcgtctc atccggcgct
      241 ttccgcaggc gcacgaagcg ccgccgccgc ccgccgtcgc gcgtgcgctc gagggcatcg
      301 ccgcgctgct gcgcggcgag cgcatcgatc tttccgccgt cgcgctcgac ctcgacggcg
      361 tgccgccgtt ccatcgccgc gtctacgagg ttacgcgcac gattgcgccg ggcgtgacgc
      421 gctcgtacgg cgagatcgcg gcgcggctcg gcgcgcctcg ttcggcgcgc gccgtcgggc
      481 aggcgctcgg cagaaatccg tttgcgatcc tggtgccgtg ccatcgcgtg ctcgcggccg
      541 gcggcaagcc cggcgggttt tccgcgaacg gcggagttgc gaccaagctc cgcctgctcg
      601 cgatcgaaac cgcgcagaac aacggcgcgc ccgcgctgcc gttgtgatcg caagcgccgc
      661 ggtccccaag cgacgaacgc cggccgaccg cctgcggctt tcaaattgct cgaccttcgg
      721 gccgcagata gaatccgaaa aatgccggcc acgaccctat ttcgacgcgg atcgatctcg
      781 cgcggggcga ggtgtcggcc gatgacggcg cgacttggcg tctcgccgtg gagttcctcg
      841 cgcgccgcgt agaacctacc gagcgagggt gctcgctgct gttcaggccc gcggcttcga
      901 gcgactactc ctcgtgatcg ccaatatgaa tccgcagcct gccaggctcg acgatccaaa
      961 gggcgccggc caaagggcga gtctccagaa cggccaatag gtctctcagg cggtcgagaa
     1021 tggctcgaag tgtcgcgtgc gggccgactt ccaaaatgac cacgcctgca gttttctctg
     1081 gcgggaagcg cagcacttgg ccaaaatctc gatcgagagt gaccagcgct cgaccctcga
     1141 cggcacaggc tttgaacaat tcctcatcgg ttgcgcctcc gagttcctga ccgcgggcgg
     1201 tcttaacatc gtggccagag gatgtgagga actccagtcc gcgccgtccg atattttcgt
     1261 cgagcttgaa cttcacgcag ctccgcctat cggcaccggc acaacacgtt cgcgcgaggc
     1321 ttcggctcca taggcgatcg ccgccagcac gtcctcatgg acgagttgcg gatactctgc
     1381 caagagctgg ctttcggtca tgccactggc cagaaagtcc agaatgagtg aaacccagat
     1441 gcgggtgtct ttgatgcagg gtctacctcc gcaaatttcc ggatcgatgc tgattcgttc
     1501 aagtagcctg ttcatggcca atctctcaac ggtcgtttcg ccactatgaa accgttttgc
     1561 gctattgcgc aatgatctcg tccgccggta gtgggtcagc ggcggcggcg cgagcaatcc
     1621 cggaacgttg ctccggagtg atcaagcgcc cgccgggggt ccgccattaa aattgctcca
     1681 aaagtcgtcc cagaacctcg tccactcatc cggatgcgcg cagaagtaca aggcgataac
     1741 ggatagcggg atagcagtca gccggtgata aaaaacgact tccatactgc acaatatgag
     1801 ccattcctca acgatgccac cgctgcctta ttcaaactga cccactgtcc gtccgccaac
     1861 cgggcctcgt cgctttccgt tttgcgctat tgcgtcggga cgctcggcag cttcgcgtgg
     1921 ggcgaggtgt cggccgatga cggcgcgacc tggcgtctcg ccgtggagtt cttcgcgcgc
     1981 cgcgtggacg ctgcctgaac gatggtgcaa ggtgccgctg ctcgaaatcg ccggctctcg
     2041 caagacgccc tggggaacgc aatggctctc gccttttatt acgcatcggg ctcgccctat
     2101 gcgtggcgcg tttggctcgc gctggagcac aagggcatcc cgtacgagcg caagacgctg
     2161 tcgttcgacg cgggcgatct caagaccccg gagtttgccg cgctcaatcc gcgccgaaaa
     2221 gtgccggtga tcgtcgacga gggcttcgcg ctctacgaat ccggcgcgat cgtcgaatac
     2281 ctcgaggaca agcggcccgg cgagccgcgg ctgttctccg ccgatctacg tcagcgtgct
     2341 ctgcaacggc gtatggtgcg ggaagcggac cagtatttcg ccgcgcccat ggagcgcctg
     2401 gtcgaggccg tgctgttcac gccgccggag cgctggtccg aggagcggat tgcggccgcc
     2461 cgcgccgacg cgcaccgtga gctcgcgatc tgggagacgg cgatcgccgg cgagtatctg
     2521 gccggcgcgt tgtccgccgc ggatttcacg ctgtatccct atgtcgcgtt ggtgctgcgc
     2581 atgggcaagc gaagacccga tctggtaacg gtcgatttga tcggcccctc gatcagcgcc
     2641 tggatgcgcc gcatggaggc gctgcccgtc acccgaaaga cctggccgcc gcactggaaa
     2701 tgagctgtgg ggcgcagttc ggcgagcctc gcgctgcccc gccacgccag caattttagc
     2761 gcaaacagca acgcggcgtg aaccatttcg atacaaccac ggcattcgac cgatgcggtt
     2821 caccgaacgt tggcaacaac gacatagtaa ggggagagcc gcaccatcgg gacttggaca
     2881 tgtccatcga tttcaagact ctgtttctgc tcacgctcga cgtcgaggcg atgttggggg
     2941 ttctgctgct gttctcgtgg atgcagaaca ccgcggtccg ggccgtcgcc tggtggggct
     3001 gcgcgtatct gctgcgatcg ctatcgatcg ggctctacgg aatgtacggc gcgctgcccg
     3061 acttgatcac gatcaccgtc gccgacgtca tcttgttcgg ctcgtatgcg gtaacctgga
     3121 ccggcgcacg catcttcgac ggccgcggcc cgcggcctgg ctcgctgatc accggggcca
     3181 cggtctggtt gctggcgtgc cagttcgccg aatttgctca ggctccggcc atccgtgcct
     3241 cactcagtgc ggccatcatt gccgcgttca tgtggctcac ggcctatgaa ttctggcgga
     3301 gccgggcgga gcggctgctg tcacgctggc cggcgatatt catcctgttc gccaacggcg
     3361 cggtgttcct gctgcgcagt ccgccgatcg cccggctggc atggccgacc gacgagcagg
     3421 tgttctccag cgcctggctc accgtgatca gcagcgaatc gctgctggcg acgatttcga
     3481 ccgccttcat cctgctcgcg atggccaagg agcgcgccga gctccgccac aagacggcgg
     3541 ccaccaaaga tccgctgacg gggctcgcga accgacgctc cttcttccag gaagcctcca
     3601 ggctgatgca gaccagcgat cgtccggtcg ctctgctgat ggtcgacctg gaccatttca
     3661 aatcgatcaa cgatcagttc ggccacgcgg cgggagacga gatcctgcgg atttttgcga
     3721 agacggcgag cgccaatctg aggccgtcgg acctcgtcgg ccggctgggc ggcgaggagt
     3781 ttgccgtcct gctgccggcg gcgtcccagg agggcgcatg gttcgtcgcc gagcggctgc
     3841 gctccgtatt tgcggccgct gctggtactg tgaatggatt gtccattcag gcgacggcga
     3901 gcgtcgggat atcgatcctc gaccccggac aggacatgag tgcactgctg gatcgcgccg
     3961 atcaggcgct ctaccgggca aaagccagcg ggcgcaaccg ggtcgccggt cctcagccgc
     4021 aattctcccc ggccgcctga gcgcgagccg ccgcccgcgg cgcggaacac gacgggcgcc
     4081 gcccgcttgc tctctgggcc ttggctgcgg ggtgcggacg ttaccggaga gccgggccgg
     4141 ataagccgtc ggaaacgcca gcgccaactc gggtctgcac cggcggatcg cgttttcctg
     4201 ctgcggactt cagcagcgtc acctcgatgc ccagccgctt ggcgtcggca aagctcagcc
     4261 aggtgacgga atccggccga gcttgcgcga cgtagatgac ggcggagtac gaaagcccga
     4321 cgcgattaag gtaggcaccc agaagcgcgt tcccgacgcc ggtcacttgc ccggatttcg
     4381 ggtcggaagc cgcatgcagc ccgatccgcg ctcccggtcc catgaagcga cgggcgccgc
     4441 ccagccatgc cagcgcgcat gcggaagcgc atcgcgcgcc cgcaggcacg agggtgtgga
     4501 actgcttcaa ccggatcatt tctcccatct gaatgcccgt taccacgctg ccgccgttgc
     4561 tttgaaagcg gaccatggcc gtcgcgagcg gggcgatttt cgtaaaaaac cgttcgccgt
     4621 cggcgacgtc caacgtgcct tgcaccacga caaccgcggg cttctccgat ccggcggatt
     4681 gcacggagat cgcggcggcc cgggcgggtc ccgccgacaa caccgcgcaa gccaggatcg
     4741 caaatcgcat cgagctttac ctgtccggca ccggatgggg acctggccac cgagccgcgc
     4801 ccgagagtct aatccccggg acgacgccgg gccagcggcc gaagcgcagt cccggccgcc
     4861 gccggtccgt gcactaagga tctctgaacc actttgcggc taggtggggg cctcggcccc
     4921 gcatccagcg gctgaaagcg ccatgttgaa ggaaagacgc cggcacgcgc gccacagcat
     4981 caatcgcgtg gccaaggtcc aatcggcggg ctcgctgccg cgagactgtc tcgtcaccga
     5041 catctcggac gcaggcgcaa ggctctacgc cgagggcatc gtgattccgg atcaattcgt
     5101 tctactgatc accggctccc agaccgtgcg tcggcagtgc cgggtggtct ggcgcctggg
     5161 caacgagatc ggcgtcgagt tcgcctgaac gcgccgcttc gcccgcgcga ggaccacccg
     5221 acatttcccg ccgcaggaat gcccggcaat tccgcgtgta tttatcggat atttttaact
     5281 cgcgcctgtc gccccatggc gggtttttcg ctacagtgga aacgaccggt atgtttcgcc
     5341 ggtgatgggg cattggggag gcacaacatg ccgaacatag cactcgattg gacgacggtg
     5401 gactgggcca atgtgggcct gctctcggcg atcgcgttcc tggcggcgct gctcggcaac
     5461 atcctgatct tcaagcaccg gctctgggct gccattctgg ccgggttgct gttcgccgcg
     5521 gcctatgtgt tcctggcgta ctatccgcac ggtcttacgg tgcccgggct caagaccggc
     5581 gcccgcaagg cggccgtggt ggtgggactg aacttgacga gccccgagat cgcgccgggc
     5641 gcgaccattc aaaacgagca ggtctaccgc ggcttcgact gcaacggcac caacatctcg
     5701 ccggagctgc gctggagcgg ggctccgagc acggcgaaaa gcttcaccgt gacgctctat
     5761 gatcccgacg ctccgaccgg cagcggctgg tggcactggg tgatcgtcaa catcgcgccg
     5821 accaccacgt tcctgccgcg caacgccggc gacccgaaaa tgggcttggc gccgccgggc
     5881 acgatccaga tccggaccga tttcggcact gtaggctacg gcgggccctg cccgccgcag
     5941 ggcgacaggc cgcatcgtta cgtgttcacc attttcgccg tcgacgtcga caagctcccg
     6001 gtcgacgaga gtgcgtccgc ggcgctcgtc ggtttcaatc tgcactccca tacgttgacc
     6061 aaggcgacgc tgaccggctt ttaccgccgc tgaccgcgag cgcgcccccg gctttttcgt
     6121 cgggcgaccc agaagatccc tcacgtcaga tcgagtagag ttcgttccgt ctcgcggcgc
     6181 agcgcgcgag aacgcggcgg ttcttgcgac ctggactacc acgcgtcgac gaccatgcgc
     6241 gtcaccgaaa tgacggaacg agccccgagg tggcgacgag ccacacgccc cccgcgctca
     6301 aggcgcaccc gacgaggacc ggcaaggtcc gtccgggcgg cgacaatttt tccagcagca
     6361 ccaatgcggt gagcccgacg acccacagca cgttcatcac cccgccgacg aacaacagcg
     6421 tcatcagcgc ccagcagcat ccgacgcagt aagccccgtg cgcgagcccg agccggaacg
     6481 atccgggcgc atcggcacgg aacccgccgt ggcggtggat gaagaacagc ggcgactggc
     6541 attgcgacag gcacgcggtt ttgagcggcg tgagctgata gatgcccgcc gcgagcagca
     6601 cgagcccgcc gagcgcactg ccggcggctc ccgacatcgg cgcgcgcagg gccgcgcgct
     6661 ccagcgccca ttgggcgctt gtcgcaatga gcgcaaagcc gatccagcac agcagatagc
     6721 cagatgcgaa ccatccggtg ggcgcgaacg gatgtccctg cagcgccgcc tgtctagcca
     6781 cccgggcata gatcaggatc atcggcgcgg cggccggcgt catcatgccg actatcatca
     6841 ccgcccacat cgtcagcacg aacaggaact cggcgacgct ccacggcctg ggcgacggtg
     6901 ccagcatggc gcccatgtcg cccatgggca tgccgtttgc ctcggtggcc ggcatcctca
     6961 tgccgaaggc cagccacagc acataggccc aggcgagcgc ggtcagaacg gcgagcgcgc
     7021 ccgccacgac cacgcggtcg cggcgaagca ggatctccag cgatccttcc gccattcgct
     7081 tcagcgtatg acgccggtct ggcacaagtg cagatcggcg aaatgcgcgt gggaatccgc
     7141 gagctcgagc cggatcgggc cgttggtctt ggcccagccg cggcccactt cggctgccgc
     7201 gaactcgaag ccgttcggca tgtcgaagcg ggcgcggtgc tcgttgcccg tgaccgggtt
     7261 gcggatcggc tcgccgcgcg cctcgaccag gtccgcgacc ttcaggcgag ccttgcgcgc
     7321 gtccacgtcg acctcgaact cgatgtcggc aaagatcgga tcgtggactt tgtcgaacgt
     7381 cgtggaaaac acctggaaca tcgtcgcgcc gggcacggta tcctggccgc cgagaatccg
     7441 aagcagcgcc tcgcgctgcg ccgcggttgc gcgcttgtcg atgatgatgc tggcttcgcc
     7501 cttgccttcg tggatggggc cgggccattt gaggatgctc accgtgcgca acccgtcgag
     7561 ccgtgtcggg ccgtgatatc cttcgtcgat ctgcatgccg accaccgcct ggcagtcgcc
     7621 atatgtcggc agggcgttga actggcaggg acagccgtag gcgcaattgc agtggccgaa
     7681 ctcccggccc ttgatcatcc agctcgcgtc cggcatcgca atctccatcc ccgcccatcg
     7741 atggcggatt cgtggagaat ccaccatact atccgccgca agggaagctg ccgacgcggc
     7801 ccgcgccggc gcatcgggcg agccgacgcg cgccgatatg tccgggcgcc gtcccggtga
     7861 gcgcccgata ggttgcgcgt tccacgacct gtccgcactg cagggatccc gatgaaacgc
     7921 caatgccccg ccctagtcgc gcttgccggc ctgcttgttg cctccgtggc gctcgcgcag
     7981 gagcgctcga tcacggtcgc ctcgacgacc tccaccgagc aatcgggtct gttcggctat
     8041 ctgctcccgc aattcaccgc caaaagcgga atccgcgtga acgtggtcgc cgtcggaacc
     8101 ggtcaggcgc tcgacatcgg ccggcgcggc gatgccgacg tggtgttcgt ccacgacaag
     8161 gcggcggaag agaaattcct cgccgagggt ttcggcgtga agcgcttcga cgtgatgtac
     8221 aacgacttcg tcgtgatcgg cccgggcagc gaccccgcgc ggatcgccgg cggcgaggac
     8281 gtgctcgagg cattgcgcaa gatcgcggcc atcaaggcgc cgttcatctc gcgcggcgac
     8341 cggtccggca cccacgccgc ggagctgcgc tattggcacg acgcgggcat cgatatcggc
     8401 gcggtcaaag gcgcctggta ccgcgagatc ggccagggca tgggcgccgc gctcaacatg
     8461 gcggcggccg ccaatgccta tctgctctgc gaccgcggga cctggctgtc gttccaaaac
     8521 cgcggcgagc tttcgatcct ggtggcaggc gaccgccggc tgttcaatca atacggcgtg
     8581 atgctggtca atccggcaaa gcaccccaac gtgaggacga gggaagggca ggccttcgtg
     8641 gactggctcg tctccccgga gggccagcgg acgatcgccg cctacaaggt cgggggcgag
     8701 cagctgttct ttcccgatgc cggcgatctg aagggatcgt gattgtaata ggccgtatag
     8761 gggccaaagc tctacggcag gtcgcgccat ggtgcgcctg atcgcaagat ccgaccggct
     8821 gccgttgcgg acacgacggt catctacacg cggcacgcgc cgtggtttat tcgggaggaa
     8881 cggcctgatg gcggccactc atagtccgtg agttcatacc gcacgattcg agcctcccgt
     8941 cttggagcct gaatcacgcc aatcccgata ccttcaacac cgaactggca tttcccagat
     9001 tacccgctaa ccggacatcg tgcgggcatc gcaaaagcga cgcgattgac ccgaagcgga
     9061 acttgcgctc cctgatatga tgggatttat cgaatccaac tggcgtcgat agatcgagtc
     9121 acgtttggcc ggttgcctaa gtgcaaccgc ctttccggcg gcccgctagg tcgcgaggca
     9181 tctttgggca agattcaacg aagcacgcca attgagagca tggctttgat cgacgtgctg
     9241 tgaacacgat agcgattacc gtcgcggtta aacggtgtag cagttcgaaa taaagaacaa
     9301 tacaaccaga ctgaccccac tcgataccgt gatggcgaca aaggtgcatg gattcttcaa
     9361 aggacaagca ggatgctcga caggcgcaca gtagccgccg cattgctcgc tggagcggca
     9421 acctcaataa ttggcgcggg caaggcgcga gcgcagactc agcctctcac gcccgcacgt
     9481 aacgtggttt tggttcatgg cctctatgct gacggatctt gctggctaag agtaatcccg
     9541 cttttgcagc aggtcggcct aaacgttacg gcggttcaaa atcctctgcg atcgttagat
     9601 gaggattgtg aattcacgcg ccgcaccctg accttgcagg atggcccgac agttttggtc
     9661 gcccactcct atggaggcga ggttgtcacg cagacggggg gcgacccgaa ggtatcggcc
     9721 ttggtgtact gctcggcacg tgcgcctgac gcgaacgagg attatacggc gctcgccaag
     9781 gcctatccta ccccgccggc gagcgcgggc ctcgttcacg ccaacggcta tgttcagctg
     9841 agtgaggagg ccttcctccg cgattttgcc ggcgacattg acccgggcac ggcccgcgca
     9901 ttgtacgcgg tccagggccg ggccgccgag cccatcttcg gcgaccgcgt gacgcaagcg
     9961 gcttggcgca caaagccatc tttctatcaa gtctcaagca aggaccgcac tatcaacccg
    10021 gaccttcagc gcttcatggc gaagcgaatg aaggccaaga ccatcgagat cgaatccagc
    10081 catcttggca ttatttcgca tccacgtaag atcgccaatc taatattgga ggcggccggt
    10141 cacgccacct gatcgcttgc gccggcccct gcatggtgtc gggaggcatc acgactctgg
    10201 cgggccccgc ctcgtcgaga cctcgcacaa acggcagcgt ctacaagccc gatgtcggtt
    10261 gtcggcacga ctgcgacatc gcaccgagcc gcctgcaatt ccgcttgcgg tgcctgagtg
    10321 gacatgtgcc ggaaacgctc ggaggaccgg gttcgaccca aaccggactc caattggggc
    10381 ggcgcataaa ggcagtcctg acaatttgag gaggcgcaac agatcgtctt acctatagcc
    10441 atcacttcgg tggttccggg cgccgcgatg aatgatggtg gataatgagc cagtcattcc
    10501 cacgcttgat agccacgaag gtaaatctag cagggacctc tagcggcttc ccatcctgcg
    10561 tctctgcaaa atcgtaaaat cccgtagcca atgccgaagt gtcgctaatg gcgatgatcc
    10621 gaagctcgcc gacgctcact ttagcgctgc ttccgggcag aaaggaaaag tacttctgaa
    10681 cggactcact gttctccgca agcgtcggac tcaacgtgcc gagcaaggtc gcgtcctgtg
    10741 catataattt tgccagtgct tgtgcatcat tggagttgaa ggcctcagcc caacgcccga
    10801 ttaacacatt tacttcatct gccggcgctg cgagcgacgg tccgggtgca tatgccagca
    10861 tcactgcgct cagcgcaatg atcgtaaacc ggttcatcgt ttccatcctc cctgccagcc
    10921 agcaagtcta agcgcccgcc gctgcatcgc ctgtaccgcc gtgatcactc cgatagagag
    10981 cggctaccga tggaccgggc gactatcgct acgatgttct cgtcattaca gtttcgctcg
    11041 ttcataattc gcgttacgca taatgtagtc acgcgacgcc tcgacgaggt tcaccatctt
    11101 gtgatagtcg acgccgacca tctggttatt gcgcttcatc accttgccgg cgatcagaac
    11161 cgtgtccacg ttggaggcgt tcatgctcgc gacgatcgcg ccggtggcgc tgttcatggg
    11221 catgacgctg aaacgatccg tgcgcaacag gattaggtct gcctccttgc ccggcgttag
    11281 ggttccaacc ttgcgatcga ggtgattggc gcgcgcaccc tcgaccgttg caaactcgat
    11341 cacgtcgcgc gccgtcagaa gcttgggcag gttcttgtca cccgccagtc cgcgtatcca
    11401 gagattgttg tgttggagcg ctaggatcgt tcgcatctgc gtgaacaagt cccccggagc
    11461 ggtggtctcc acgtcaatcg agagtgaagg ccgcagtcca aggtcgagca ccttctggac
    11521 cggcggcgtg ccgtctccca tgatcatctc gacatagccg gccagcgaaa acgtgccgcc
    11581 gctgtcggcg atcatcttga actcggtgtc gcttaaggtg cagcagtgga tgtaggtcgt
    11641 atcggctttc agcacgccct tgtccccgag cttttgcaca acgccggtga tcgcattgcc
    11701 gccccctaca tggatagtga tcggcgcccc tacttcgcgc gcgaccgccc acgcctctgc
    11761 cacccggtcg ggcggattgg acgggctcgc aagtgccagc gtaagcaact gatcgctgct
    11821 ggaaaaacac tgtttgcgca gacggcggat atcgtccgga tatttctgcc gcggatcatc
    11881 tcgaaaatcg ggaaggccgt tggatgggat gccataggcg aacacagcgc ggataccgga
    11941 atgtgcaagc gcttcgatcg cggcatcggt gtgctcgggc gaattttgga tatgtgacca
    12001 atcgaggatc gtagtgatgc cgacttctat ggcgccgagc gcgctgacca ggttcgccgc
    12061 gtagacatcc tcggggcgat agtgcgggcc gagctggagc tgcacgatct tgaggtaatc
    12121 gtcgagggat gcatccggaa gcatgttgcg caggatgcct tcccacatgt gccgatgcgt
    12181 atcgatgaac ccgggcatga cgatcgcgcg gccggcgtcg atgacttccg catcgggcgc
    12241 gctgaggtcg ggcccgactg cggcgatctt gctgccggaa atcagcacgt cggcgcgttc
    12301 gaagtcgcca accgtgggat cgagtgaaag cacgcaaccg ttcctgatca ggatcggccg
    12361 atccgatccc ggcgccctgc tcggctgtgc ctgcgcgctc gccacagtgc tcgccacgag
    12421 ggccggaacc gcggccgcag ccgcgactcc cgtgcacagg aacccgcgcc gcgaaagcgt
    12481 acgggatgat ccgcaagcac agcaaacatc gcgtcgggca tccgatatcg gcgccatcgt
    12541 ctcctcctga gagcgctggc cgtaagggct tgcagcgcta tggctgttgt ccagttcagc
    12601 ttgtgatgtt tagggctctt ttatatcagc gacccgccac aatcatctga aaattggctc
    12661 gtgaaatcaa cgcccagtac gtctcccctt tcccggcaac cccgctcttc acttagccag
    12721 ccacggacgc tcccacgagc gtggctgttg cgttttgagg caagtctggc ggcggccccg
    12781 gatcgtaatg ctgcgcggcg ccggtgccgg cctacatacg cccgagggca gcgactcgtg
    12841 cgatttacct gcggtgcccc tggcccctcg ctccacacac ggcaacttgc ctggtgggca
    12901 aagggtgctg aagacccgga tcgctggagc tggggttatc tggggtcatg ggtaatccga
    12961 agcgcctggg cgagatcagc cagccttcgc acgctgcgcg cgtggaaacc ggaggggctg
    13021 acccgtccag caccggtcgg ccgcgccacg gccgggatcg gcctgatgtg aattagacgg
    13081 aggcgtttag acgggtgtcg gtgcggggct ggccttcgtc ttctcgacct tctttgacca
    13141 ggaccggtag gcggcaacgc cggtcatcag cgcgacgccc acgagactca ccagcacctg
    13201 catccagatt tcattcgaca cctcgacgag cacaaaatac gccagggacg acagaaagat
    13261 tccgacgcag aacacttcga gcgactgctg cccgcacacg atcaacggcc acgctaccgg
    13321 cctttcgagt gcgcgccagt cgcgaggcac gaaccggacg atgagaaagg cgatcaccac
    13381 gaaatgcagg aagcgataag gggcgagatt ggtcttgtcg ttgggattga acgtctcgta
    13441 gagccacgcc ggcagcagct ctcgtagctc cggaaaccgg ccggccatgg tcatgacgag
    13501 ggcgaacagc agataggcgc agcccaatgc gggtagcgcc ttcgagcgga tcagcggcat
    13561 cgattgtgac gtcccaccca gcgcgaacca tgcaccgaac acgaacagga gctgccagca
    13621 gaacgggttg aaataccaga ccccgatcgg gtacgacggc aggttccagt cgaaatggcg
    13681 cgcggcgaaa tacagcagcg ccgaaatcgc gagcacgagg tttagccggc gcagcatcat
    13741 ccacaatacc ggcgcgaagc acgccatcag cacgatatag aggggaagca cgtccatatt
    13801 gacgggcttg aatttaagga tcagcccctc gtaaagcgtg gtgaacgggt cgcgtatgaa
    13861 gccgaagaca ttgaatttct gggccagcac gggattgtcg taccgggtcg cgagccaccc
    13921 gatctccgcg acatagatca cgaacaacag aacgtgcgcg acatagatct gccacgcgcg
    13981 ccgccctagt cgcgtggcgc cgatgaggaa gccgcgttcg agcatgaccc gcgcgaaaac
    14041 gaatgaggcg gtgtagccgg aaatgaacac gaacaggtcg gccgcatcgc tgaaaccgta
    14101 gttccgcggc gtgatccaat tcacaatgtt gttggggatg tgatcgagga agatccacca
    14161 gttggcaaca ccgcggaaga agtcgagcct gagatcgcgg cccttaggcg gcagggtgac
    14221 ggacatcggg gcgctcccgg acgatggagg atacgagctt gccgcgcggc gccgcggatt
    14281 gcaccattat tttctctatc cagaggtcgc cgcacacgtg atgcgagtga cggagaccgc
    14341 gcactggctc gagtggcagg actgggccga tcccattttg cagaagccct acgatataaa
    14401 gaacggcctg ctgcacattc ccgaagttcc cagagtaagc ctggagtggg atgaagacgc
    14461 aatggcggcc aaccaagacc ggctctagcc gcgctgaggc gaacacgagg gattagtctg
    14521 gaaagacagc tgttggcacg actgcgacat cgcactgtgc ggcccgcaat tccgcttaag
    14581 gtccctgagc ggacgtgtag cggaaacacc cggaggaccg ggtttgaccc atctcggaca
    14641 ttggcggact cgaagtaatg tcgtgccggt ttgatgatat gcttccattg cgatacctga
    14701 acagccgcgt catactgcaa gatatcgcac cattcatcgt agaaagtcgt gagaaagttg
    14761 gcgagccaca gggggaagct tatgcttaag acgccgatgt cgccttcacg cgcattggcg
    14821 cgacctgacg tttccgaccg atgtatcgat cccgcttctc aaggacgtca aggcacaggc
    14881 gccgaacgcg gtgtgcgtgg tcggggcaga tcgggagcag cgtcatggac aagaattcgt
    14941 ttttatggaa aatggtcgat ggccggctgc cgctgtctcc ttgcgacaag acgctgggct
    15001 tcgagttcgt ccagatcgat ggcaagcgcg gcacggtcga aataaaattc gaagcgaagc
    15061 cggaattcct aaaccccgca ggaaacgtgc agggcggatt tctcgccgcc atgctcgacg
    15121 acaccatggg accggcgctg gcggcgatgc tggacgccgg cgaattcgcg ccaaccctaa
    15181 atttgaacgt gcagtttcat cggccggcca aggtagggcc gttgagaggt gtgggacgcg
    15241 tggtattgcg tggcaaggaa atttgccagc tgagcagtga attgttccag aacgacaaga
    15301 tcgtcgcgac cgcgacggcc acggccgtga ttcgtaaaat gtaggggaat gagatggcgg
    15361 ctatggggcg cctcattccc gcgcgtttcg agtgatctgg ctggcaaacg aggtcagcat
    15421 cccgccaatg ttcgccagtt cgcatcgctt ccgatgttgg cacttttcgg accttgcgcg
    15481 atgtccgact tgagtccgga atgcgctcca aagcggacgt tcgcccaacg cctctgaatt
    15541 tatgggttca cgccctaaat aca
//
LOCUS       DASWGM010000340         6442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_161221,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000340 DASWGM010000000
VERSION     DASWGM010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161221"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VGA15_09830"
     CDS             complement(<1..223)
                     /locus_tag="VGA15_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588031.1"
                     /translation="MLQSQVEKVPGAPKLALVKVADGFHDPVGVTAANDGSGRIFVVE
                     RAGRVRIVGKDGKVLPDPFIDLTNFNPLGS"
     gene            complement(477..908)
                     /locus_tag="VGA15_09835"
     CDS             complement(477..908)
                     /locus_tag="VGA15_09835"
                     /inference="COORDINATES: protein motif:HMM:NF040028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6220 domain-containing protein"
                     /protein_id="HEX9588032.1"
                     /translation="MARSGMSERHGLPATANLLLRFLSGLLTVSILIQFFIAGMSSLT
                     APDWWNYHKIWVNIFQWLVLPLPVFAWFCGKPRGGRVLFASLPILQIALQYVLVHRAL
                     DGRLPNGIGLHAVNAALMLVVATLLTLGWHDGNNLFKNRQI"
     gene            complement(890..1747)
                     /locus_tag="VGA15_09840"
     CDS             complement(890..1747)
                     /locus_tag="VGA15_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EboA domain-containing protein"
                     /protein_id="HEX9588033.1"
                     /translation="MLNEFLALADGWISERTDERGAAWFRGAINEVAKASNERPLSVA
                     IGLAPRRLGKADLALPAADLARAEALRPGLDLSDWSIDQLARVALMAGSHGGNDADFA
                     ARFDSFCTTAEINELIALCRGLPLYPNAALIEPRAREAVRSGMKPVFEAVAHRNPYPR
                     ETFAEDAWNQMVVKAFFIGSTLWPIQGLDERANPRLARMLVGLAQERWAAERPVSGEL
                     WRCVAPHADDEGVAALAHGWNAGNDRERLAIALALRGAPNARSLPPEEVAKFQARLES
                     EKPEWRDLA"
     gene            complement(1797..2675)
                     /locus_tag="VGA15_09845"
     CDS             complement(1797..2675)
                     /locus_tag="VGA15_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX9588034.1"
                     /translation="MSMTATAMPGLSTLLRLGRISNVPTVWTNVLAGAVIAGGAQSPN
                     EVALIVLAMTAFYVGGMYLNDYFDRAIDARDRPGRPIDAGEIRAGSVLSIGFGLLALG
                     VALMMSFGPRAILCGLLLAGVIVLYDVWHKGNVLSPVVMGLCRALVYIGTGVAATGGV
                     SNATIIGAVALACHVAGITYAAKQESLDRVENLWPLALLAVPLFAALPVLFSGWIVIA
                     AFVLLLAADAFAIWLLAKQPVQGAVPLAVSGLIAAICLVDALAIAAAGGGIFIVAVCA
                     VGYPLTRLFQRSIPGT"
     gene            complement(2672..3874)
                     /gene="eboE"
                     /locus_tag="VGA15_09850"
     CDS             complement(2672..3874)
                     /gene="eboE"
                     /locus_tag="VGA15_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HEX9588035.1"
                     /translation="MKLDQLAGNVNLTYCTNIHAGESWPDIRASLDAHVPAIKAAVAP
                     DRPLGIGLRLSGEAAAVARQPEQLAAFREQLATLGGYVFTINAFPFGPFHGVRVKEHV
                     YLPDWRSAERVAFTADSAAVLAAVLPDGVDGSISTVPGAFKANGRADGAVAAIAANMM
                     KAVADLVLTERRTGKRIALALEPEPCCFLETTDESLAFFQEVLLQPEALDLLAAGIGE
                     NRGGAEAMLRRHLGICYDICHGSVEYEDPVAALDRLLAAGISVPKIQLSAAMRVPAMT
                     DNLIGAVMRYDDGVYLHQTIVRGPSGLTRYVDLPDAVAAFKEGRANGEWRIHCHVPLF
                     LSDLGEIGSTRADLVSTLAAFRRKTRSSHLEVETYTWDVLPENLRTGSKAADIAREIS
                     FCMQELVG"
     gene            complement(3871..5064)
                     /locus_tag="VGA15_09855"
     CDS             complement(3871..5064)
                     /locus_tag="VGA15_09855"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX9588036.1"
                     /translation="MLDTPSNTISDVRVQTFNIAYEYPVVFTRDAFSSGNRSLIDVLA
                     RRESGKVHRCGIFVDEGVLSAIPDLTDRIADYASFHAKHLKIIGEPVAVPGGERCKND
                     PNTVPRLLAKFSELAIDRHSFVIAIGGGAVLDAVGYAAAIFHRGVRHIRFPTTVLAQD
                     DSGVGVKNAVNALGSKNLVGTFAPPWAIINDSAFIDRLPLREKRAGMAEAVKVALIRD
                     GNFFRWIESNTVALAQFSPPHLDRLIKDCAELHMRQIRLGGDPFEAGSARPLDFGHWS
                     AHKLEHLTRNEVSHGEAVAIGIALDTRYSVMTGLLAEGEDRRVLGVLERLGFTLWHGA
                     LRQQNAKGKPALLQGLADFQEHLGGELTVTLLADIGHGIEVHEMNAEMVGRSIDWLQQ
                     QAQAR"
     gene            5316..>6442
                     /locus_tag="VGA15_09860"
     CDS             5316..>6442
                     /locus_tag="VGA15_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase/phosphodiesterase
                     family protein"
                     /protein_id="HEX9588037.1"
                     /translation="MKSVLAMIVVGLTPRHLGALTPRLSALAREGAMAPLQTVTPAVT
                     CSVQASFMTGLTPREHGIVANGWLFRDLMEVMLWRQSNRLVEGEKVWEAAKRRDPAFT
                     CANMFWWYNMASSHDFGATPRPIYKADGRKLPDCYTVPDSLRNRLTERLGPFPLFQFW
                     GPATTIASTRWIADATKLVMAENNPTLTLAYLPHLDYDLQRFGPDETSPAVKKSLAEI
                     DTVAGDLADAARASGRSVVILSEYGITKVDRPIHINRALRDAGFIAVREEDGAELLDP
                     MASKAFAVADHQIAHVYVSDPRSLPKVREVLEDLPGIERVYDATDKHSLDLDHPRSGE
                     LVALADARSWFTYYYWNDDRRAPDFARTVEIHRKPGYDPVEL"
BASE COUNT         1301 a         2026 c         1927 g         1188 t
ORIGIN      
        1 ccgagcccag cggattgaag ttggtgagat cgataaacgg atccggcaga accttgccat
       61 ccttgccgac gatcctgacg cgcccggcgc gttcgacgac aaagatacgc ccggagccat
      121 cattggctgc ggtcacgccg accggatcgt gaaatccatc ggccaccttg acgagcgcca
      181 atttcggggc gcccggtact ttttccacct ggctttgcaa catccgccgg cgtcgccggc
      241 gcctgctgtg cataggccgc aggtatgccc atcgttgtcg ctgccaaaaa gacagcgagc
      301 gtcatcgctc tcacgatgat tgctctcctc gccctgttcg tttcccggcc tgcaattttt
      361 aattttctct gcggccaact tagaacgata tcgagctccc tctaacctgc gatagaaaat
      421 gttttgggaa gccgaccatc aattgtttta aatttaagat caggaaaata tcccgactat
      481 atttgccgat tcttgaagag attattgccg tcatgccagc ccagcgtaag caaggtcgcg
      541 acgaccaaca tcaatgcggc gttgaccgca tgaagtccga tgccgttcgg taagcggcca
      601 tcgagtgcgc ggtgtacgag aacgtattgg agcgcgatct gtaaaattgg caacgatgcg
      661 aagagaacgc ggccgccgcg gggcttcccg caaaaccagg cgaataccgg caacggcaat
      721 accagccact gaaaaatgtt gacccaaatc ttgtgatagt tccaccaatc cggcgccgtc
      781 agcgacgaca tgcctgcgat gaagaattga atcagaatgg aaacggtcaa caatcctgac
      841 agaaatctca ggagcaggtt cgccgttgcc ggaagaccat gccgctcgct catgccagat
      901 cgcgccattc gggtttttct gattctaatc tcgcctgaaa cttcgcgact tcttccggcg
      961 gcaagcttcg agcgttcggc gctccccgca gcgcgagagc gatcgcgagg cgttctctgt
     1021 cgttacctgc attccagcca tgcgctaatg ccgcaacgcc ttcgtcatcg gcgtgcggcg
     1081 cgacgcatcg ccataactct ccgctgacgg ggcgctccgc cgcccaacgc tcttgtgcca
     1141 gtccgaccag catgcgcgcg agccggggat tagcgcgttc gtccaatccc tggatcggcc
     1201 aaagcgtcga gccgatgaaa aacgctttca ccaccatctg attccacgcg tcttccgcaa
     1261 aggtctctcg cggatacgga ttgcgatggg cgacggcctc gaacaccggc ttcattccgg
     1321 agcgcaccgc ttcgcgcgcg cgcggttcga tcagcgccgc gttggggtaa agcggaagcc
     1381 cgcggcacag tgcaatcagt tcattgattt cggcggtggt gcagaagctg tcgaaccgcg
     1441 ccgcgaaatc ggcatcattg ccgccatgac tgcccgccat cagcgcgaca cgcgccagtt
     1501 gatcgatgct ccagtcgctg agatctagcc ctggacgcaa cgcttccgca cgcgccagat
     1561 cggccgccgg cagcgccaga tcggctttgc cgagccggcg tggcgccaga ccgatcgcaa
     1621 cgctgagcgg ccgctcattc gaggccttcg ccacttcatt gatggcgcct cgaaaccagg
     1681 cagcgccgcg ctcgtccgtt cgttcggaga tccacccatc ggcaagcgcg agaaattcat
     1741 tcagcacggc agtctcttgg gtttttggga ggatccgaac gcgggcatga cacccttcat
     1801 gtgccgggaa tcgacctctg aaaaagccgc gtcagcggat agcccactgc gcagaccgcg
     1861 acgatgaaga taccgccgcc ggcggccgca atcgccagcg catcgaccag acagatcgcc
     1921 gcgatcaatc cggatactgc cagcggcact gcgccctgca cgggctgctt cgccagcaac
     1981 caaatcgcaa acgcgtcggc cgccaacagc aggacgaaag ccgcgatgac tatccatccg
     2041 ctgaacagaa ccggcagcgc tgcgaacaac ggcacggcca gcagcgccag cggccagagg
     2101 ttttccaccc ggtcgaggct ttcctgtttg gccgcatagg taataccggc cacatggcac
     2161 gccagcgcaa ccgcgccgat gatggttgca ttcgagacgc cgccggtcgc cgcaacgccg
     2221 gttccgatat agaccagcgc tcggcacaat cccatcacga ccgggctcag cacatttccc
     2281 ttgtgccaga catcgtagag cacgatcacg ccggccagca gcaggccgca caggatcgca
     2341 cggggaccaa acgacatcat cagcgcgacg ccgagcgcca gcaggccaaa accgatcgac
     2401 aacaccgagc cggcgcgaat ttcgccggca tcgatcggac ggccggggcg gtcgcgcgca
     2461 tcgatggcgc ggtcgaagta gtcattgaga tacatgcccc cgacgtagaa ggcggtcatc
     2521 gcgagaacga tcagcgcgac ttcgttcggg gactgcgcgc cgccggcgat gacggcgccg
     2581 gccagaacgt tggtccagac cgttggcacg tttgaaatcc ggccgagacg cagcagcgtc
     2641 gacaaaccgg gcattgcggt ggccgtcatg ctcatccgac gagttcctgc atgcagaatg
     2701 aaatctcgcg cgcgatatcg gctgccttgg agccggttcg tagattctcc ggcagcacgt
     2761 cccaggtata ggtctcgact tccagatgcg acgagcgtgt tttccgtcga aacgccgcca
     2821 gcgtcgacac gaggtcggca cgggtcgatc cgatctcgcc aaggtcggat aggaacagtg
     2881 gcacatggca atgaattcgc cattcgccgt tggcccggcc ctctttgaat gcagcgacgg
     2941 catccggcag atcgacatac cgcgtcagcc ccgaaggccc tcgcacgatg gtttggtgca
     3001 aataaacgcc gtcgtcatag cgcatcaccg cgccgatcag gttatcggtc atcgcgggaa
     3061 cgcgcatcgc cgcggagagc tggatcttgg gcaccgagat gccggcggcc agcaggcggt
     3121 cgagtgctgc gacaggatct tcatattcga ccgacccgtg gcagatatcg tagcagatgc
     3181 cgagatgacg gcgcagcatg gcttcggcgc cgccgcgatt ctcgccaatg ccggctgcga
     3241 gcaggtcgag cgcttcgggc tgcagtagca cctcctggaa gaacgcgagg ctttcatccg
     3301 tggtctcgag gaaacagcac ggttcgggtt ccagcgcgag ggcgatgcgc ttgccggtcc
     3361 gccgctcggt cagaacgaga tcggcgaccg ccttcatcat gttggcggcg atcgcggcga
     3421 cggcgccatc tgcgcgccca ttggccttga atgcgcccgg cacagtggag atgctgccgt
     3481 cgacgccatc cggcagcacc gcggcaagga cggcggcgct gtcggcggtg aaggcgacgc
     3541 gctcggcgct gcgccaatcc ggcagataaa catgctcctt gacgcggacg ccgtggaagg
     3601 ggccgaacgg aaaggcgttg atggtgaaga cgtagccgcc gagcgttgca agctgctcgc
     3661 gaaacgctgc cagttgctcc ggctgccgcg caaccgcggc cgcttcgccg gacaggcgca
     3721 ggccgatgcc aagcgggcga tccggcgcca ccgcggcttt gatcgcaggg acatgtgcat
     3781 cgaggctggc acggatgtcg ggccagctct cgccggcatg gatatttgtg caataggtca
     3841 ggttcacatt acctgcgagt tggtcaagtt tcatcgggcc tgcgcctgtt gctgaagcca
     3901 atcgatactg cggccgacca tctctgcatt catctcgtgc acttctatgc catggccgat
     3961 gtccgcaaga agcgtgacgg tcaattcgcc gccgagatgc tcctggaaat ccgccagccc
     4021 ctgcagcaaa gcgggtttcc ccttggcgtt ttgctgtcgc aacgcaccat gccacagcgt
     4081 gaagcccagc cgttcgagaa cgccaagtac gcgccggtct tcgccctccg ccagcaatcc
     4141 cgtcatgacc gaataccggg tatcgagcgc gatgccgatc gcgaccgcct cgccatggct
     4201 gacttcgttg cgcgtgagat gttcgagctt gtgggcggac cagtgtccaa aatccagcgg
     4261 ccgcgctgac cccgcttcga acggatcgcc accgagcctt atctggcgca tatgcaattc
     4321 cgcgcaatcc ttgatcaggc gatcgagatg gggcggcgaa aattgcgcca gcgccaccgt
     4381 attgctttcg atccagcgaa agaaattgcc gtcgcggatc agcgccacct tgacggcctc
     4441 ggccatcccg gcgcgttttt cgcgcaacgg cagacgatcg ataaaggcgc tgtcgttgat
     4501 gatggcccag ggcggcgcga aagtgcccac cagatttttc gagcccagcg cgttgactgc
     4561 attcttgacg ccgacgccgc tgtcatcctg cgccagcacg gtggtgggaa aacggatatg
     4621 gcgaacgcca cggtgaaaga tcgctgccgc atagccgact gcgtccagca ccgcgccgcc
     4681 gccgatcgcg atcacaaagg aatgccgatc gattgccagt tcggagaatt tcgccagcag
     4741 ccgcggaacg gtattgggat cgttcttgca gcgctcaccg ccgggcacag cgacgggttc
     4801 gccgatgatc ttgagatgct tggcatggaa cgaggcataa tctgcaattc gatccgtaag
     4861 atccggtatc gcggacaaca cgccctcatc gacaaaaatg ccacagcggt gaaccttgcc
     4921 gctttcccgg cgcgccagaa cgtcgatcag gctgcgattg ccggaactaa aggcgtcgcg
     4981 cgtgaacacc acgggatatt cgtaagcaat gttgaatgtt tgcacgcgaa cgtcgctgat
     5041 cgtgttcgac ggtgtgtcga gcatggcact ctccctgata cgacacctcg tcttgtccag
     5101 gaacgggtgt cgccactcgc ttttttaggc actggcgggt tcggtagcgc cgccagtgat
     5161 cgcgggtttg ttttttcaat caacaaggtg ccacttcacg gatggacttg gcaagccggt
     5221 ttgcgcggcc gaaaaaacct gcaaggatga cattgcccgt tctgaacgac ggccaaagac
     5281 caagaaaatg cgaattaccc ccggggagag atgatttgaa atcggttctg gcgatgatcg
     5341 tggtcgggct cacgccgcgc catctggggg ctctgacgcc gcggctttct gcactcgccc
     5401 gcgaaggcgc catggcgccg ctccaaaccg tgacgcccgc cgtgacgtgc agcgttcagg
     5461 ccagtttcat gaccgggttg acgccgcgcg agcacggcat cgtcgccaat ggctggctgt
     5521 tccgcgacct gatggaagtg atgctgtggc ggcaatccaa ccgcctggtc gaaggcgaaa
     5581 aggtatggga ggccgcaaag cggcgcgacc cggcgttcac ctgcgccaac atgttctggt
     5641 ggtacaacat ggcatccagc catgattttg gcgcaacgcc gcgaccgatc tacaaggcgg
     5701 acgggcggaa gctgccggat tgttacaccg tgccggacag cctgcgtaat cgtctgaccg
     5761 aacggctcgg gccgtttccg ctgtttcaat tctgggggcc ggcgacgacg atcgcatcga
     5821 cccgctggat cgctgacgcg accaagctag tgatggccga gaacaatccg acgctgacct
     5881 tggcctacct gccgcatctg gactacgact tgcagcgttt cgggcccgat gaaaccagtc
     5941 ccgcggtcaa aaaatctctc gccgaaatcg acaccgtggc tggcgacctt gccgatgcgg
     6001 cgcgtgccag cgggcgttcc gtcgtgatcc tgtccgaata cggcatcacc aaagtagacc
     6061 gtccgatcca cattaaccgc gcgctgcgtg acgccggatt tatcgcagtg cgggaggaag
     6121 acggcgccga attactcgac cccatggctt cgaaagcgtt tgccgtcgcg gaccatcaga
     6181 ttgcgcatgt ctatgtgtcc gacccacgct cgctgccgaa agtgcgagaa gtgctagaag
     6241 atctgcccgg aattgaacgc gtttatgatg ccactgacaa gcatagcctc gatctcgatc
     6301 atccgcgctc gggcgaactg gtggcgttgg cggatgcgcg ttcctggttc acctattatt
     6361 attggaacga cgatcgccgc gcgccggatt ttgcccgcac cgtcgagatc caccgcaagc
     6421 ccggctatga tccggtggag ct
//
LOCUS       DASWGM010000341         6306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_865964,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000341 DASWGM010000000
VERSION     DASWGM010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865964"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VGA15_09865"
     CDS             complement(<1..572)
                     /locus_tag="VGA15_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX9588038.1"
                     /translation="MCGRFVITSPPAALRQIFGYIEQPNFPPRHNIAPTQPIPVVIVE
                     NGIRHFRLMRWGLLPAWVKDPSKFALLINARGETVLEKPAFKNAMKRRRCLIPADGYY
                     EWQVSEKRKRPYFIHRRDGQPIGLAGLAETWIGPNGEELDTVAIVTAPASPDLAVLHH
                     RVPVTIEPDDFERWLDCRDDDAEKVMALLVA"
     gene            635..1150
                     /locus_tag="VGA15_09870"
     CDS             635..1150
                     /locus_tag="VGA15_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588039.1"
                     /translation="MTSTASQPVRSDRPMPQPTGLDKEARAAQLRAANINPRTGLATD
                     YLNHFNEAIMLLEMIPDMPECAEDFLSWHPLSYAEHFTASHFKARDLAIEAYESADAT
                     VRAEFDNITGAMTSILTAVSAAMREAKQDKTRATLAEQATGWVKPLVALAGGIINGRG
                     DETDVDYIMTH"
     gene            1288..1719
                     /locus_tag="VGA15_09875"
     CDS             1288..1719
                     /locus_tag="VGA15_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9588040.1"
                     /translation="MSTASPAQPSHPQLAVSAAIFRDGKVLLVRRARSPAKGFYSLPG
                     GRVEFGESLHTALHREVDEETALKIEIIGLAGWREVVPGTSGGGHYLIMSFAARWIAG
                     EPVLNDELDDFRWLEPDGLSDLKVTGGLFEVVQSARGLIRG"
     gene            1801..2178
                     /locus_tag="VGA15_09880"
     CDS             1801..2178
                     /locus_tag="VGA15_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HEX9588041.1"
                     /translation="MFKHFLAALILISACHAGPARAQDAAAPFDGDLQRLAEILGTLH
                     YLRGICGTNEGPKWRNEMQALIDAETPSGDRRARMIAGFNRGYNGFQQTYRTCTPAAS
                     VAIRRYIEEGSKISRDLTARYAN"
     gene            2206..2676
                     /locus_tag="VGA15_09885"
     CDS             2206..2676
                     /locus_tag="VGA15_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588042.1"
                     /translation="MTGVAGPWTAEKLAALAPVDTVVQLARKMILAKNYPGVDVLTAR
                     SRLLDDHRVDAYHLDIADRARRAIAALRIAEETGNSAADSILLERARKLQAFFSQPFF
                     VAEPFAKRPGSYVSRADAVRGCAEILNGRHDDLPVDAFYFTGSIEEIRGGRAPA"
     gene            2830..3096
                     /locus_tag="VGA15_09890"
     CDS             2830..3096
                     /locus_tag="VGA15_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588043.1"
                     /translation="MSLPASFPAARDPLPDQEQKQAALGYLNEAWAEARHDGVDGDCL
                     AQVSLFAAFAELVGSYGEDAVATYVEGLPARIRNGEFSTARARQ"
     gene            complement(3122..3253)
                     /locus_tag="VGA15_09895"
     CDS             complement(3122..3253)
                     /locus_tag="VGA15_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588044.1"
                     /translation="MTPTSDVVTRLDRVIQYAAASMLKYEGSGILGRPVKPGDDSFA"
     gene            complement(3290..4624)
                     /locus_tag="VGA15_09900"
     CDS             complement(3290..4624)
                     /locus_tag="VGA15_09900"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX9588045.1"
                     /translation="MNQRFDTILKSGTVVNQDGEGVRDIAVTDGRIAAIGGLGQASAA
                     EVIDCKGLHILPGVIDTQVHFREPGLTQKEDLETGSRSAVMGGVTAVFEMPNTDPLTV
                     TEQTFADKVKRGHHRMHCDFAFFIGGTRDNVQDLPELERAPGCAGVKVFIGSSTGALL
                     VEDDESLRRIFKVIRRRAAFHAEDEYRLNDRKGLRIEGDPRSHPVWRDETAALLATQR
                     LVNLAHETGKRIHVLHISTKEEIEFLRDNKDVASCEATPHHLTLVAPECYERLGTRAQ
                     MNPPVRSADHRDGIWRGIEQGIIDVLGSDHAPHTLQEKAKTYPASPSGMTGVQTLVPL
                     MLDHVHAGRLSLARFVDLTSAGPARLYNIAVKGRIAAGYDADFTIVDLKRSETITNKW
                     VASRAGWTPYDGVRVTGWPVGTFVRGRRVMWQGELMTPSTGEPVRFLETLKA"
     gene            4799..5680
                     /locus_tag="VGA15_09905"
     CDS             4799..5680
                     /locus_tag="VGA15_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX9588046.1"
                     /translation="MKAAFLPDRGVVKVSGIDARDFLNGLVTTDVALLRPGLGRFGAL
                     LTPQGKITVDFLITEAPSGHGGGFLIDCPRALAQGFADKLGFYKLRAKVAVENLSDSL
                     GVLAAWDGDPSVMPDLSFADPRHAALGWRILIPVELAQKVADLIGSELVDSGAYDAHR
                     IASGVPRGGLDFMYGDAFPHETNMDRLHGVDFEKGCYVGQEVVSRMQHRGTARTRTIR
                     VILESASPEPGAAILAGDKSVGTMGSTAGPNGLALIRVDRVAEAVAAGLPLTSGGLAI
                     RVAEPDDLLIAPKQTVA"
     gene            5677..6303
                     /locus_tag="VGA15_09910"
     CDS             5677..6303
                     /locus_tag="VGA15_09910"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX9588047.1"
                     /translation="MTRSARLHADGLVRCPWPGEDPFYMAYHDTEWGVPEYDDRALYE
                     KLILDGFQAGLSWITILRKRDNFRRAFDDFRPEKIARYNAKKLQALMNDAGIVRNRAK
                     IEGTVASAKGYLKIMEDGPGFSKFMWDFVDGRPKVNNFKTTASVPASTPLSIKMSKEL
                     ASRGFKFVGPTIVYAFMQATGMVNDHLVTCFCHETCAGKHRIPRLKVK"
BASE COUNT         1171 a         2008 c         1944 g         1183 t
ORIGIN      
        1 gcgaccagca gcgccattac cttctctgca tcgtcatcac ggcaatcgag ccagcgctcg
       61 aaatcatcgg gctcgatggt cacgggcacg cggtgatgta ataccgcgag atcagggctc
      121 gcgggcgcag taacgatcgc cacggtatcg agttcctcgc cgttggggcc gatccaggtt
      181 tccgccaacc cggcaaggcc aatcggctgt ccgtcgcggc gatggatgaa ataaggccgc
      241 ttgcgctttt ccgagacctg ccattcataa tagccgtccg ccgggatcag gcagcgccgc
      301 cgcttcatgg cgttcttgaa ggccggtttt tccagcacgg tttcgccgcg tgcgttgatc
      361 agcagggcga atttactggg atccttgacc caggccggca gcaggcccca gcgcatcagg
      421 cggaaatgcc ggatgccatt ctcgacaata accaccggga tcggctgggt cggcgcaata
      481 ttatgccgtg gcgggaagtt gggctgctcg atatagccaa agatctgtcg caaagccgcc
      541 ggcggcgagg taattacaaa gcgtccacac attctgaggc ccagataggc atgcgttcag
      601 caccttttaa ccccaaccgt taacactgcg gcagatgaca tcaacggctt cccaacccgt
      661 gcgatccgat cgcccgatgc cgcaaccgac gggccttgac aaggaggcgc gggctgcgca
      721 gttgcgagct gccaacatca acccccgcac tggtctggcc accgattacc tgaatcattt
      781 caacgaagcc atcatgctgc tcgagatgat tccggacatg ccggaatgcg cggaagattt
      841 cctgagctgg caccctctgt cctatgccga gcatttcacg gcgtcccatt tcaaggcgcg
      901 cgatctcgcg atcgaggcct atgaatccgc cgacgcaacc gttcgtgccg aattcgacaa
      961 tatcaccggc gcaatgacct ccattctcac cgcggtgagc gccgccatgc gcgaggccaa
     1021 gcaggacaag acccgcgcca cgctggccga acaggccacc ggctgggtca agccgctggt
     1081 cgcactggcc ggcggcatca tcaatggccg cggcgacgag accgacgtcg actacatcat
     1141 gacgcattag atcatgatcc gattaggtag aatcgaatca ttgagcatga tctccgcgca
     1201 aacgcgttcc gcgtttgtcg cgagggaaaa ccggcttcca cccatcgggg cgtggcccga
     1261 gggcatgctt ttccggatca tgctctgatg tccacggcgt ccccggccca gccgtcgcat
     1321 ccccagcttg ccgtcagtgc ggcgatcttt cgtgacggca aggtcctgct ggtacggcgc
     1381 gcgcggtcgc cggccaaggg tttctattcg ctgcccggcg gccgggtcga attcggcgaa
     1441 tcgctgcaca cggcgctaca tcgcgaagtc gacgaggaaa ccgcgctgaa aatcgagatc
     1501 atcggcctcg ccggatggcg cgaggtggtg ccgggaacca gcggcggcgg gcactatttg
     1561 atcatgtcat tcgcggcgcg ctggatcgcc ggcgagccgg tcctgaacga cgaactcgac
     1621 gatttcaggt ggcttgagcc ggatggctta agcgatctca aggtcaccgg aggcctattt
     1681 gaggtcgtcc agtcggcccg cggcctgatc cggggctgac tctggatcgc gtcccaagcc
     1741 cttcgagcct tgcttccagc gcattgagaa ggcatatcac ggacgtcccg gatccccttc
     1801 atgttcaagc actttctggc cgctctcatc ctcatctcgg cctgccatgc cggcccggcg
     1861 cgggcccagg atgcggccgc gccgttcgac ggcgacctgc agcggctggc cgaaatcctc
     1921 ggcaccctgc actatttgcg gggcatttgc ggcaccaatg aaggccccaa atggcgcaac
     1981 gaaatgcagg cgctgatcga tgccgaaacc ccctccggcg atcgccgcgc ccgtatgatc
     2041 gcgggcttca accgcggtta taacggcttc cagcagacct accggacctg cacgccggcg
     2101 gcttcggtcg cgatccgcag gtacatcgag gaaggttcga agatctcgcg ggatctgacc
     2161 gcccgttacg ccaactaggc gcgttttcaa ggctgcgtgc gctttttgac cggcgtggca
     2221 ggaccgtgga cggccgagaa gctggcagca ctggcgccgg tcgatacggt ggtgcaactc
     2281 gcgcgcaaga tgatcctcgc caaaaactac ccgggagtgg atgtgctgac ggcgcgctcg
     2341 cgtctgctgg acgatcatcg cgtggacgcc tatcatctgg atattgctga ccgtgcccgc
     2401 cgggccatcg cggcgttgcg catcgccgaa gaaaccggaa attccgccgc agattccatc
     2461 ttgctggaaa gggcgcgaaa gctgcaggcc tttttctcgc agccgttctt cgtcgcagaa
     2521 ccctttgcca agcggccggg ctcgtatgtg agccgtgccg acgccgtgcg cggctgtgcc
     2581 gaaattctca acggccggca cgacgatctg ccggtcgacg cgttttattt tacgggaagt
     2641 attgaggaaa tccgcggcgg cagggctccg gcctgagcag gtcaggcgcc gttcggaaac
     2701 ccgatcaaaa tatccaaagc gccgttaacc tttcctaaag aactggccta ggcggccccg
     2761 aatcgcatgg taaacaccgc gcgttcgatg cgttccgccc tggatcgcgc ttccagcctg
     2821 ttgagtttca tgagccttcc agcgtcattc ccagccgccc gcgacccgtt gcccgatcag
     2881 gaacagaagc aggccgcgct cggctatctc aacgaggcct gggcggaggc gcgccacgat
     2941 ggcgtcgatg gcgactgcct ggcgcaggtg agcctgttcg cggcgttcgc cgaactggtc
     3001 ggaagctacg gcgaagacgc ggtcgcgacc tatgtggaag gcctccctgc ccgcatccgc
     3061 aacggcgagt tctcgaccgc gcgagcgagg cagtgagttt gcacgaagca gtaagcttgc
     3121 atcaagcaaa gctgtcatcg cccggcttga ccgggcgacc cagtattcca gagccttcgt
     3181 attttaacat cgacgccgcg gcgtactgga tcacccggtc aagccgggtg acgacgtccg
     3241 aggttggcgt catgtcccgc tttaacgtga aacatgagag cgcaacgaac taagccttca
     3301 gcgtctccag aaaccgcacc ggctcgccgg tcgagggcgt catcagctcg ccctgccaca
     3361 tcacgcggcg gccgcgcacg aaggtgccga ccggccagcc ggtgacgcga acgccgtcat
     3421 aaggcgtcca gcccgcacgc gaagccaccc atttgttggt gatggtctcg ctgcgcttga
     3481 gatcgacgat ggtaaagtcc gcatcatagc ccgccgcgat ccggcccttg acggcgatgt
     3541 tgtacagccg cgcgggcccg gcgctggtga ggtcgacaaa gcgcgccaac gacaatcgcc
     3601 cggcatggac gtgatcgagc atcagcggca ccagcgtctg caccccggtc atgcccgacg
     3661 gcgaggccgg ataggttttg gccttttcct gaagcgtatg cggcgcgtga tcggagccga
     3721 gcacgtcgat gatgccctgc tcgatgccgc gccagatgcc gtcgcggtga tcggccgagc
     3781 gcaccggcgg attcatctgc gcgcgggtgc cgagccgctc gtagcattcg ggagcgacca
     3841 gcgtgaggtg atgcggcgtc gcttcgcagg aggcgacgtc cttgttatcc ctcaggaatt
     3901 cgatctcctc cttggtcgag atatgcagca cgtgaatccg cttgccggtt tcatgggcga
     3961 gattgaccag acgctgggtc gccagcagcg ccgcggtctc gtcgcgccac accggatgcg
     4021 agcgtggatc gccttcgatg cgcaacccct tgcgatcgtt gaggcggtac tcgtcctcgg
     4081 catgaaaggc ggcgcggcgc cggatcacct tgaagatccg ccgcaggctt tcgtcatcct
     4141 cgaccaacag cgcgccggtt gacgagccga tgaacacctt gaccccggcg cagcccggcg
     4201 cgcgctccag ctccggcaga tcctgcacat tgtcgcgggt gccgccgatg aagaacgcaa
     4261 aatcgcagtg catgcgatga tggccgcgct tcaccttgtc ggcaaaagtc tgctcggtga
     4321 cggtcagggg atcggtgttc ggcatttcga acaccgccgt gacgccgccc atgacggcgc
     4381 tgcgcgaccc ggtttccagg tcctcctttt gggtcaggcc gggctcgcga aaatgcacct
     4441 gggtgtcgat cacgccgggc aggatgtgta ggcccttgca gtcgatcacc tcggcggcgg
     4501 aggcctgccc aagcccgccg attgctgcaa tgcgaccatc cgtgacggcg atgtcgcgca
     4561 cgccctcgcc gtcctgattg accacggtgc cggatttcag aatggtatca aatcgttgat
     4621 tcatgggtcc tcagcacccg ctttacggaa ccgatgtcgc gcgggccttg ttcccgatac
     4681 cttagcgtct tacgttgcca tgtgatatcc ggcgctggcg tttccccaag aacttcgaag
     4741 agcacttcga gcgaacttcc aagcgaacct tcaaaagaac tttgtcagag aactccttat
     4801 gaaagcagcg tttcttcccg accgcggcgt ggtcaaggtg agcggcatcg acgcccgcga
     4861 ctttctcaat ggccttgtca ccacagacgt tgcgctgctt cgcccgggcc tcggccggtt
     4921 cggcgcgttg ctgacgccgc agggcaagat cacggtggat ttcctgatca ccgaagcgcc
     4981 ttcgggccat ggcggcgggt tcctgatcga ctgcccgcgc gcgctcgcgc aaggttttgc
     5041 tgacaagctt ggcttctata aattgcgcgc caaggtcgcg gtcgaaaatc tctcggacag
     5101 tctcggtgtg ctggcggcgt gggatggtga tccttcggtg atgcccgatc tttcgttcgc
     5161 cgatccgcgc catgccgcgc tcggctggcg gattctgata cccgtggaac tcgcgcaaaa
     5221 ggtcgccgat ctgatcggct cggagctggt cgacagcggc gcctacgacg cccatcgcat
     5281 cgcctcgggc gttccgcgcg gtgggctcga ctttatgtat ggcgacgcgt ttccgcacga
     5341 aaccaacatg gatcgcctgc acggcgtcga tttcgagaag ggctgttacg tcggtcagga
     5401 ggtggtgtcg cggatgcagc accgcggtac cgcccgcacg cgaacaatac gggtcattct
     5461 cgagagcgcg tcgccggagc cgggtgctgc cattctggcc ggcgacaaat cggtgggcac
     5521 catgggctcg acggcaggcc ccaatggcct cgcgctgatc agagtggatc gcgtggccga
     5581 ggcggttgcc gccggactac cgctgacatc gggcggcctc gccatccgcg ttgccgagcc
     5641 ggacgatttg ctgatcgcac cgaagcagac cgtggcatga cgaggtcggc gcgcctgcat
     5701 gccgatggcc tggtgcggtg tccgtggccc ggcgaagatc cgttttacat ggcttatcac
     5761 gacaccgaat ggggcgtgcc ggaatatgac gatcgcgcgc tgtacgaaaa gctcattctc
     5821 gacggttttc aggccgggct gtcctggatc acgatcctgc gcaagcgcga caactttcgc
     5881 cgcgccttcg acgatttccg ccccgaaaag attgcgcgct acaacgcgaa aaagcttcag
     5941 gcactgatga acgacgccgg catcgtccgc aaccgcgcca agatcgaggg cacggtcgca
     6001 agcgccaagg gctatctgaa gatcatggaa gacggcccgg gcttctcgaa attcatgtgg
     6061 gatttcgtcg acggcaggcc gaaggtcaat aacttcaaga ccaccgccag cgtgccggca
     6121 tcgacgccgc tgtcgatcaa gatgtcaaag gaactggcgt cgcgtggatt caaatttgtc
     6181 ggcccgacca tcgtctatgc cttcatgcag gccaccggca tggtcaacga ccatctggtg
     6241 acctgctttt gccatgaaac ctgcgccgga aaacatcgca tcccgcgcct caaagtcaaa
     6301 tgacgg
//
LOCUS       DASWGM010000342         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_577670,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000342 DASWGM010000000
VERSION     DASWGM010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577670"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VGA15_09915"
     CDS             complement(<1..254)
                     /locus_tag="VGA15_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588048.1"
                     /translation="MMTMISPPKLSIPIAAAAVTFCLVCGLGLGLCFGGVGAVAAQSM
                     PDTENGRYTLSPVADGVLRLDTRTGAVSTCNNNGTGWACYA"
     gene            complement(305..400)
                     /locus_tag="VGA15_09920"
                     /pseudo
     CDS             complement(305..400)
                     /locus_tag="VGA15_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
     gene            complement(480..1049)
                     /locus_tag="VGA15_09925"
     CDS             complement(480..1049)
                     /locus_tag="VGA15_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX9588049.1"
                     /translation="MKTALVSRYHPLLVTLHWLLAVLIIAMLCIGFVVLAPMPNTDPQ
                     KIGILLVHMSVGMAILALMVARFIVRMRTLRPVDATTGYPRLDRLAPITHYGFYVLVL
                     LMVGSGYATAILAGLNKSVFQGSGDPLPPDFTVYPTFVAHGTLAALLAGFIVLHVLAA
                     FFHQFVLNDGLLRRMLFGRRGPNSSAQVK"
     gene            complement(1046..1489)
                     /locus_tag="VGA15_09930"
     CDS             complement(1046..1489)
                     /locus_tag="VGA15_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9588050.1"
                     /translation="MTDAGTATHSIVIERVMPHPPERIWRALTQGPLIEAWLMKNDFQ
                     PVVGHRFNLRSTPMPHWNGVVDCEVLVVEPNARLSYRWNASGEEAATGIKTIVTWTLT
                     PANSGTLVRMEQSGFRPEHAGNYQGAKYGWQKYFAGLEQVAAGVP"
     gene            complement(1486..1809)
                     /locus_tag="VGA15_09935"
     CDS             complement(1486..1809)
                     /locus_tag="VGA15_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9588051.1"
                     /translation="MPIASTELFSALADPTRRAIFERLARDGEQTVRVPTGQAGVSQP
                     AVSKHLAVLKLAGLVLDRRDGRQTHYSAQPQGLTPLLDWISLYGAFWRDRFDRLETLL
                     NRMDQ"
     gene            2079..2459
                     /locus_tag="VGA15_09940"
     CDS             2079..2459
                     /locus_tag="VGA15_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX9588052.1"
                     /translation="MAEGTDDSGSGSRDQSSSDEAALSARLGSLDQRLSEIRGSRKIG
                     TDPSGNEQDTAQARASAMAVGLRLSSELVAGVLVGAALGWGFDRLLSTSPWGLIVFLL
                     LGFVAGVVNVMRAAGVMAKQSERL"
     gene            2536..3288
                     /locus_tag="VGA15_09945"
     CDS             2536..3288
                     /locus_tag="VGA15_09945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9588053.1"
                     /translation="MAADPIHQFQITKLFTIGHIGGQEIAFTNSSAYMFLSVAVISLL
                     MIGGSAGRQLVPGRIQSMAELSYEFVASTIRSNCGSEGMKFFPLVFSLFMFIMVSNLV
                     GIIPYNFTVSSHLIVTAALALLVFLTVLFYGLYKNGLKFFKIFVPSGVPIYILPLVVF
                     IEIISFFLRPVSHSVRLFANMLAGHIALKVFASFVAMLGALGILGWAGAVLPLGLTMA
                     LTALELLVAFLQAYVFAILTCIYLNDAIHPGH"
     gene            3330..3557
                     /locus_tag="VGA15_09950"
     CDS             3330..3557
                     /locus_tag="VGA15_09950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX9588054.1"
                     /translation="MDPIAAKYIGAGIACIGMGGAGAGVGIIFGNYLAAAVRNPSAAQ
                     GQFGNLIFGFAVTEALGIFSLLIALLLLFAL"
     gene            3624..>3975
                     /locus_tag="VGA15_09955"
     CDS             3624..>3975
                     /locus_tag="VGA15_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HEX9588055.1"
                     /translation="MAESHGDAKGATAHTEAEGGHKAPFPPFQKDTYASQLVSLLIAF
                     VALYLIVSKIALPRVGGLLEERQKKIEGDLAAAQKLKDESDAALKAYESELAAARSRA
                     QAIGTETREKLNAAS"
BASE COUNT          788 a         1215 c         1188 g          784 t
ORIGIN      
        1 gcatagcagg cccatcccgt cccgttgttg ttgcaggtcg aaaccgcgcc ggtcctggta
       61 tcgagcctga gcacgccatc ggcgaccggc gacagcgtat aacggccgtt ctcggtatcc
      121 ggcatcgatt gcgcggcgac ggcgcctact ccgccaaagc aaagaccaag acctagacca
      181 cagacgagac aaaaggtcac cgccgcggcc gcgatcggga tggagagctt cgggggcgag
      241 atcattgtca tcatgggggt tgctccgcct caaaccgatc aagtttgcta ttccaggctt
      301 gacctcacgc ttgcgagcga tagggcgcac ggccggactt caacgcgtca gacagcggtt
      361 cgatccgatc ctggccccag aatacctcgc cgtcgagcac gttttcggct ttcatcccaa
      421 cagacccgac agtccgatgc aagatcgatc atcggacggc agttcgctgt tttggtcgat
      481 tatttcacct gagccgagga gtttggcccg cgccggccaa acaacatccg ccgcaacaat
      541 ccgtcgttga ggacgaactg gtgaaaaaac gcggcaagca cgtgcaagac aatgaagccg
      601 gcaagaagtg cggccagcgt gccatgcgcc acgaaggtcg gatagaccgt aaagtccggc
      661 ggcagcggat cgccggaacc ctggaacacg cttttgttta acccggccag gatcgccgtc
      721 gcatatccgc tgccaaccat caggagaacc aggacgtaga agccgtagtg cgtgatcggc
      781 gccagccggt cgaggcgcgg ataacctgtc gtcgcatcca ccggccgcaa tgtccgcatt
      841 cgcacgatga agcgcgcgac catcagcgcc aggatcgcca ttccgaccga catatggacg
      901 agcagaatgc cgatcttctg cggatcggtg ttcggcatcg gcgcaagcac gacaaacccg
      961 atgcacagca tggcaatgat gagcacggca agcagccaat gcagcgtcac cagcagcgga
     1021 tgatagcggg ataccagagc cgttttcatg gcacgcccgc cgccacctgc tccaggcctg
     1081 cgaaatattt ctgccagcca tatttcgcgc cctggtaatt gccggcgtgc tccggccgga
     1141 agcccgactg ctccatacgc acaagggtgc cgctgttcgc cggcgtcagc gtccaggtga
     1201 cgatggtctt gatcccggtc gccgcctctt cgcccgaagc attccagcga taggagagcc
     1261 gcgcattggg ttcgacaaca agcacctcgc aatcgaccac gccattccag tgcggcatcg
     1321 gggtgctgcg caggttgaac ctgtggccga cgaccggctg gaaatcattc ttcatcagcc
     1381 acgcctcgat caacggacct tgcgtgagcg cgcgccagat tctttccggc ggatgcggca
     1441 tcacccgttc gataacgatt gaatgcgtag ccgtgccggc atcggtcatt ggtccatcct
     1501 gttcaacaag gtttcgaggc gatcgaaccg gtcgcgccag aacgctccgt aaagactgat
     1561 ccagtcgagc agcggcgtca gaccttgcgg ttgggcgctg tagtgagtct gacgtccgtc
     1621 gcggcggtcg agcacgaggc cggccagttt caaaaccgcc agatgtttgg acaccgcagg
     1681 ctgcgaaacg ccggcctgac cggtcggcac ccgcaccgtc tgctcgccgt cgcgcgccag
     1741 ccgttcgaag atcgcgcgac gcgttggatc ggcaagggct gaaaaaagct cggtcgatgc
     1801 aatgggcata aaaacccata accaagcagt tatgaataag tcaagccgga ttgaaaaatc
     1861 gcgaaccagc atttgccgat cgcccgcggc gctattgcac cgcggcgacc acttcaggcc
     1921 ggcgggcgaa aagtggaata aatcaactat atcagatgga tgcgtggtta tgacgcaatc
     1981 ttgacttggt tagatgcggt cagtatggtc cgcgcggctt ttgagggtga cggggcgtaa
     2041 tttccatcaa ccgcagcatt gtttcgggtc tctacagcat ggctgaaggc acggacgaca
     2101 gcggaagtgg cagtcgcgac caatcgtcgt ccgacgaagc cgcgctttcc gcaaggctcg
     2161 gaagtctgga tcagcggttg tccgaaattc ggggcagccg aaagatcggg actgacccgt
     2221 ccggaaacga gcaggacacg gctcaggcca gggcatccgc catggccgtc gggcttcgcc
     2281 tctcctccga actggtcgcg ggtgttcttg tcggagcggc tctcggctgg ggtttcgatc
     2341 ggttgctgtc gacttcgccc tggggcctca tcgtattttt gctgcttggc tttgtcgccg
     2401 gggtggtcaa tgtgatgcgg gcggcgggcg tgatggcgaa gcagtctgaa aggctctgac
     2461 gcgtgcctgt tgaagcgccg ctgaacgttc gacacgaccc gcgagagatc gcgaaaccga
     2521 cagaccgaga gacgaatggc cgccgatcca attcatcagt tccagatcac caagctcttc
     2581 accatcggtc acattggcgg acaggaaatc gcgttcacca attcatcggc ctacatgttt
     2641 ctttcggtgg cggtgatctc gctgttgatg atcggcggct ccgccggccg gcagctggtt
     2701 cccggccgca ttcagtcgat ggctgaactg agctacgaat tcgtggcgag caccatcaga
     2761 agcaactgcg gctcggaagg catgaagttc ttcccgctgg tgttctcgct gttcatgttc
     2821 atcatggtct cgaacctggt cgggatcatt ccctacaact tcacggtttc aagccatctc
     2881 atcgtcaccg cggcgctggc gctgctggtg ttcctcaccg tgctgtttta cggcctctac
     2941 aagaacggcc tgaagttctt caagatattc gtgccgtcgg gtgttccgat ctacatcctg
     3001 ccgctggtgg tgttcatcga gatcatatcg ttcttcctca ggcccgtttc gcattcggta
     3061 cgactgttcg ccaacatgct ggcgggccac atcgcgctga aagtgttcgc gagcttcgtc
     3121 gcgatgctcg gcgccctggg aatcttgggc tgggccggcg ccgtgttgcc gctcgggctg
     3181 accatggcgc tgaccgcgct cgagttgctg gtcgcgttcc tgcaggccta cgtcttcgcc
     3241 atcctcacct gcatctacct caacgatgct atccatccgg gccactgatt aaggctcatc
     3301 tcgacaaacc caaagaagga gttcgtttca tggatccgat tgccgcgaag tatattggcg
     3361 ctggtattgc ctgcattggc atgggtggcg ccggcgcagg cgtgggcatc attttcggca
     3421 actatctcgc cgccgcggtg cgcaatccgt cagcagccca gggccagttc ggcaacctga
     3481 ttttcggctt cgcggtgacc gaagcgctcg gcatcttctc gctgctgatc gcgctgctgc
     3541 tgctgttcgc gctctgatcc atcatcgatc tggccagtga gattctaagg ttcgaatccg
     3601 cattgaccga acaggagaag cccgtggctg agagtcatgg cgacgccaag ggcgcaaccg
     3661 cccacaccga ggcagagggc ggccacaagg cccccttccc gccgttccag aaggacacct
     3721 atgcttcgca actggtgtcg ctgctgattg cgttcgtcgc actttatctg atcgtctcga
     3781 aaattgcgct gccgcgcgtc ggcggcctgc tcgaggaacg ccagaaaaag atcgagggcg
     3841 atctggccgc ggcgcaaaag ctgaaggacg agtccgacgc tgcgctgaaa gcctatgaga
     3901 gcgaactcgc cgccgcgcgc tcgcgcgcgc aggcgatcgg caccgagacc cgcgaaaagc
     3961 tcaacgcggc ctccg
//
LOCUS       DASWGM010000343         8692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_577806,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000343 DASWGM010000000
VERSION     DASWGM010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577806"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGA15_09960"
     CDS             <1..373
                     /locus_tag="VGA15_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HEX9588056.1"
                     /translation="TIEAKVSYLSPVGSSDTQSALARAVVPNSEQRLRPGLFITGRLT
                     LSAKQVGVAVKSSALQTIENRTVVFVRDGEKFEARDVEIGERDSELVEITFGVLEGDV
                     YAARNSFIVKAELAKGSPDND"
     gene            427..3636
                     /locus_tag="VGA15_09965"
     CDS             427..3636
                     /locus_tag="VGA15_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX9588057.1"
                     /translation="MIDGMLSFAIRQRWLVMIGVLAMAAFGAWNFTRLPIDAVPDITN
                     VQVQINSRAPGYSPLEVEQRITFPIETAMGGLPNLENTRSQSRYGLSQVTAIFKDGTD
                     IYFARQLVNERIQQVRDQLPAGIETAMGPVSTGLGEIYMFTVEARPEARKTSGEPYTP
                     SDLRTIQDWIIRPQLRTVPGLSEVNTVGGFEKQFHVLPDPAQLMAYKLSFRDVMTALA
                     ANNANVGAGYIERNGEQYLVRTPGQVGNIDEIKEIVIGSRNGVPVRVSDIADVREGTD
                     LRTGAATLNGKEVVLGTAMLLIGENSRTVAQRVAAKLMEIGRSLPDGVIAHTVYDRTR
                     LVEATVATVEKNLVEGALLVVVVLFLTLGNFRAAIATACVIPLSMLFAITGMVENRVS
                     ANLMSLGAIDFGIIIDGAVIIVENCLRVLAAEQHRLGRLLSRQERFETILAGSREVIR
                     PGLFGTFIIAVVYLPILTLTGVEGKMFTPMALTVLMALTGASILSLTFVPAAVALLVT
                     GNVSKHENFFMRGARRIYAPLLAASIRNGFGITVIAALLVAVCSIAASRMGGEFIPGL
                     DEGDVSMEVIRIPGTSLTQSVEMQAMLERRLLKVPEVKEAFARTGSAEVATDPMPPSG
                     SDGYVMLKPRKEWPDPEKSKAVVVSDIQEACEDVPGSVYGISQPIQQRMNETVSGIRS
                     DVAVKIFGDDLDVLIQSAARIQAILKNVQGAEDVKTEQTSGLPVLTVNLNRQALSRYG
                     ISVGDVQNLVEIAVGGKGAGRVFEGDRRFDIVVRLPEHLRSDIKAIKSLPVPLPPIDN
                     QAKATPALWGNSPLAQIRYVPLSELAQINLAPGPNLISREDGKRRIVVTANVRGRDLS
                     SFIADAQNQVMGKVKLPAGYWIGWGGQFEQLVSATQRLTIVVPLALFLIFLLLFISLG
                     SVPDALLVFSGVPLALTGGVAALLLRGIPLSISAGIGFIALSGVAVLNGLVIITFIEK
                     LRREGHALVDAVREGALTRLRPVLMTALVASLGFVPMAIATGAGAEVQRPLATVVIGG
                     VISSTILTLLVLPALYVLFRRENRSNTAAVADANQGERP"
     gene            3633..4004
                     /locus_tag="VGA15_09970"
     CDS             3633..4004
                     /locus_tag="VGA15_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588058.1"
                     /translation="MRFILAIIAALSMASPAVAEEYEKGPNGGLMLDVAGIDAELLTS
                     GNSVTINVFEAFSPKPISTKGYAAAVLIVSGANREPLTLTQSGENSLKGEAKKPIPPD
                     ATITLTIKTEAGKSGQVRFKK"
     gene            4365..4721
                     /locus_tag="VGA15_09975"
     CDS             4365..4721
                     /locus_tag="VGA15_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588059.1"
                     /translation="MLATVLGLIFEFASGRIIDIGAVVFGLATWIVPPLLAALSWNKK
                     RSSLASVGFTIYLFVAAACLSAVGLSLALLTLIFAQDANWAWLALLAIAAFWLSGIIL
                     ISVGERRHRRAREVSK"
     gene            complement(4757..5194)
                     /locus_tag="VGA15_09980"
     CDS             complement(4757..5194)
                     /locus_tag="VGA15_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9588060.1"
                     /translation="MTRYLLDTNIISNVTKPTPSAALLAWMAEQADSDLFISALTVGE
                     IRRGLLEQPKGKKRAVLEAWFAGPDGPQALFAGRVLPFDEKAGLIWARLMADGTAKGR
                     PRSALDMIIAAVAEANGCLVVTDNEKDFIGLKFVNPLRSRRAE"
     gene            complement(5191..5493)
                     /locus_tag="VGA15_09985"
     CDS             complement(5191..5493)
                     /locus_tag="VGA15_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588061.1"
                     /translation="MANTAQKRALRNYRTRLVKRGMARFEVLGLDSDRDLIRSLAKRL
                     AEDDPDALRIRAAVSRTITGEPPKKGGILAALRRSPLVGADLSLARSHEAGRKVDL"
     gene            complement(5624..>8692)
                     /locus_tag="VGA15_09990"
     CDS             complement(5624..>8692)
                     /locus_tag="VGA15_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="negative regulator of septation ring formation"
                     /protein_id="HEX9588062.1"
                     /translation="AQAIYSIDSRLTKLTSSLTDGTAQAIEAVDRRISNVTETIDGRS
                     AQLTNTITARFQEIHQGIETRVGAVATDIDMRVSQFEDLLGSRVEAVAGRIESSGRQA
                     SDDLMARAEELSLGIKSHVEDAERSLTNLVVNTSETIQTGARAAQQSLLTVSSDVGAQ
                     LKMTSAEVERALTAVGSGAANSILTSARDAQTTLTTASGEAAAQIKSLSADVERTLSA
                     AGTATASSVVAGAREAQTTLVSASADAASQVKSLAADVERTLSAAGTATASSVVAAAR
                     EAQSTLVAASSEAANHVKSLAADTAASILGSAREAHLSLTATSGEAANQIKAISADIE
                     RSLGAVTASTTDNIQTSALNAQIALVAVSNEVSSKVKSTSAEVERSVLAASGTFSSTM
                     TGKTDEVVTYVQQQTDRLAQMIDGKRGSLVEAIGAKANQLTVDIDRVTSDSLKSIETR
                     GLAFSQSMTTSGSDVARTITNAGELATTAVSKSLKDLEQASRTAIDQSRQVSIAAVTE
                     MQETSKILRTDTVALFERLREGNILLQEVLTGAHDNLNSLERALVTRVADFVSAMNDV
                     TSRNGIATQTLEDQLSIFNTKTSRALEDLGSLSSQFDSHGKALVEAAGLVEQSNRDTS
                     ISVAERKAELESLVTTIDLRTADLDHRLSRFTGLLDESLAAAESRARDIARVVAETAG
                     AGSAAISRQFESVRASAEDERRRTTDAMSEMYQQTTQEADAMFKQSADKFAAMVSSMK
                     QMALEMHNELEATRNELRRGVLEMPQEAAESTSQMRKVIVDQIEALAELNRIVARHGR
                     GLDVVSAGRTSAPRDEEPMQMHAVAAAGRSEAPAPVRTAPPRPREAASSASSLPPPDL
                     GAPASRRTEAPPVSPANPEQGRDGWLSDLLNRADVPGSNREAPRGRAPQTPGVGANPL
                     ESLSLDISRMMDRNLASEMWDRYQRGESKAFTKRLYTPAGQKAFDEVARKYRADRTFK
                     QTVDRYITEFERLLDEIARDDRGPAALRSHLVSETGMVYTLLAHAAGRLG"
BASE COUNT         1521 a         2657 c         2776 g         1738 t
ORIGIN      
        1 gacgatcgag gccaaggtgt cgtatctgtc gccagtcggc agcagcgaca cccagagcgc
       61 gcttgcaaga gcggtcgtgc cgaattccga gcaacggctg cggcccggcc tgttcatcac
      121 cggccggctg accctatcgg cgaagcaggt gggagtcgct gtgaaatcct cggcattgca
      181 gaccatcgag aaccgcaccg tcgtcttcgt ccgcgacggc gagaagttcg aggctcgcga
      241 cgtcgagatc ggcgagcgcg attcggagct cgtggaaatc acatttggtg tgctggaagg
      301 tgacgtctac gcggcacgga acagtttcat cgtgaaagcg gaattggcga aaggctcgcc
      361 ggacaatgat taatgtcacc ccgatgctgg ccaccgttct cataaacaga tgttcggaga
      421 agtgcgatga ttgacggcat gctttccttc gccatccgtc agcgatggct ggtgatgatc
      481 ggcgttcttg ccatggcagc ctttggtgca tggaatttca cgcgcctgcc gatcgacgct
      541 gttcccgaca tcaccaatgt tcaggtccag atcaacagcc gtgcaccagg ctactctccg
      601 ctggaagtcg aacagcgcat cacgtttcca atcgagaccg ccatgggcgg gttgccgaac
      661 ctcgagaata cacggtcgca atcgcgctac ggtctcagcc aggtcacggc catcttcaag
      721 gacggcaccg atatctattt cgccagacaa ttggtcaacg aacggattca gcaggtcagg
      781 gatcagttac ccgccggcat cgaaaccgcg atggggccgg tgtccaccgg actgggtgaa
      841 atctacatgt tcacggtcga agccaggccc gaggctcgca agacgagcgg ggaaccctac
      901 actcccagcg acctgcgcac gattcaggac tggatcatca ggcctcagtt gaggaccgta
      961 cccggcctca gcgaggtcaa cactgtcggc ggattcgaaa agcagtttca cgtgctgccc
     1021 gatccggcgc aactgatggc ctacaaactc agcttccgcg acgtcatgac ggcgcttgcg
     1081 gcaaacaatg cgaacgttgg cgccggctac atcgagcgca acggcgagca atacctcgtc
     1141 cgcaccccgg gacaggtcgg aaacatcgac gaaatcaagg aaatcgtgat cggatcgcga
     1201 aacggcgttc cggtgcgggt gtccgatatc gccgacgtca gggaaggcac ggatctgcgc
     1261 accggtgcgg caacgttgaa cgggaaggaa gtcgtgctgg gcaccgcaat gctgctgatc
     1321 ggcgaaaaca gccgcaccgt ggcgcagcgc gtggccgcca aactcatgga gatcgggcgt
     1381 tcgttgccgg acggcgtgat cgcccatacc gtctacgaca ggacgcgcct cgttgaggcg
     1441 acagtcgcga ccgtcgagaa gaatcttgtg gaaggcgctc tcctggtggt cgttgtcctg
     1501 ttcttgaccc ttggaaactt cagggctgcg atcgcaaccg cgtgcgtcat tccgctgtcc
     1561 atgctgttcg ctatcaccgg catggtggag aacagggtca gcgccaactt aatgagcctt
     1621 ggcgccatcg acttcggcat catcatcgac ggtgcggtca tcatcgtcga gaattgcttg
     1681 cgtgtgttgg cggcggagca gcaccggctc ggccgccttc tcagccggca ggaacgtttt
     1741 gaaaccattc tggcgggatc gcgcgaggtc atccgccccg gcctatttgg aacattcatc
     1801 attgccgtcg tttacctgcc gatcctgacg ctcaccggcg tcgaaggaaa gatgttcacg
     1861 ccgatggcct tgacggtcct gatggcgctg actggggcca gcatcctgtc gctaacgttc
     1921 gtgccggcgg ccgtagctct gttggtcacc ggcaatgtat ccaagcatga gaatttcttc
     1981 atgcggggcg cgcgccggat ctatgcgccg ctgctcgccg cctcgatccg caacggattc
     2041 ggcatcaccg tgatcgcggc cctgctggtt gccgtctgca gtatcgccgc ctcccgcatg
     2101 ggcggtgaat tcattcccgg tctcgacgaa ggtgatgtgt cgatggaggt catacgcatc
     2161 ccgggtacaa gcctgacgca atcggtggag atgcaggcga tgcttgagag gcgactgctc
     2221 aaggtgccgg aagtcaagga ggcattcgcg cgcacgggct cggcggaggt tgcgaccgac
     2281 ccgatgccgc cgtcggggtc ggatggctac gtcatgctga agccacggaa ggagtggccc
     2341 gatcccgaga agtcgaaggc cgtcgtcgtt tcggatattc aggaggcgtg cgaagatgtt
     2401 cccggaagcg tctatgggat ttcgcaaccg atccagcaac gcatgaacga aacggtatcg
     2461 ggtatccgca gcgacgtcgc cgtcaagata ttcggtgacg atctggacgt gctcatccaa
     2521 tccgcggctc ggattcaggc gattctgaag aacgttcaag gggcggagga tgtcaaaaca
     2581 gaacagacct cgggattgcc ggtgctcact gtcaatctga accgtcaggc cctgtcacgc
     2641 tacgggatca gcgtcggcga tgttcagaac ctggtggaaa tcgcggttgg cggcaagggc
     2701 gcaggccgtg tgttcgaagg tgaccgccgt ttcgatatcg ttgtcaggct tccagagcat
     2761 ctgcgctcgg acatcaaggc gatcaagtcg ctgccggtcc cgctgccgcc catcgacaat
     2821 caggccaagg caacgcccgc tctgtggggc aattccccgc tcgcccaaat ccgttatgta
     2881 cccttgtcag aactcgccca aatcaatctg gcgcccggcc ccaatctaat cagccgtgag
     2941 gatggcaaac ggcggatcgt ggtgactgcg aatgtgaggg gccgcgacct cagttcgttc
     3001 atcgccgatg cccagaacca ggtcatggga aaggtgaaac tgccggccgg ctactggatc
     3061 gggtggggcg gccagttcga gcaactggtc tcggcgacgc agcggctgac gatcgtcgtg
     3121 ccgctcgcgc tgtttctcat tttcctgctg ctgttcatca gcctggggtc ggtgcccgac
     3181 gcgctgctgg tgtttagtgg cgtgcccctt gcgctgaccg gaggggttgc agcgcttttg
     3241 ctgaggggaa tcccgctctc catcagtgcc gggatcggct tcatcgcgct gtccggcgtg
     3301 gcggtgctca acggcctggt catcatcacg ttcatcgaaa aattgcggag ggaaggacat
     3361 gcgctcgtcg acgcggtccg ggaaggcgcg cttacgcgct tgcggccggt cctgatgact
     3421 gcgctcgttg cctcgctggg gtttgttccg atggcaattg ccaccggtgc cggcgcggaa
     3481 gtgcagcggc cgctcgcgac cgtcgtgatc ggcggggtta tttcgtcgac catccttaca
     3541 ttgctggtgt tgcccgccct ctatgtgctg ttcaggcgcg agaaccggtc gaacaccgct
     3601 gctgtcgcag atgccaacca aggagaacga ccatgagatt catcctggca atcatcgcgg
     3661 ccctctcgat ggcttcaccg gccgtggccg aggaatatga aaaaggcccg aatggcggcc
     3721 tgatgctgga cgtcgccggc atcgatgccg aacttctgac atccggcaat tccgtcacga
     3781 tcaatgtctt tgaggcgttt agcccaaagc cgatctcgac caagggctat gcggcagcgg
     3841 tgctcattgt cagcggcgct aatcgtgagc cgctgacgtt aacccagtcg ggcgagaaca
     3901 gcctgaaggg cgaggcgaag aagccgatcc cgcccgatgc aacgatcacc ttgacgatca
     3961 agaccgaggc cgggaagtcc ggtcaagtcc gcttcaagaa gtaaagatag atatgacagg
     4021 tttgtcggac caaccatgaa ctatcaaatt cgcgatgaat accgcgcgcg agatggccac
     4081 ggcaggtcct cgtgcttgcc aattccacgg cggccccaag ctgcctgcga gagcccgctc
     4141 ataggcctgt gaaccgattt gtcctggctt gcgacgaagt tacgtgagcc ttttccgttt
     4201 cgatagaatc gaaacggggc tccagattct tgatttgacg cgttttcttg acgcgaaccg
     4261 gtctccaccc cggatcaagt ccggggcagg ctttcgcttg aaaacgctct agccaggagg
     4321 catcgcgcaa cgtggcagtc gtgagaccaa tctttggggc cgcattgctg gcgacggtgc
     4381 tcggcctgat cttcgaattc gcgtccggtc gcatcatcga tatcggcgct gtggtgttcg
     4441 gcctcgcgac ctggatcgtt cctccgcttc tggcagcttt atcctggaac aaaaagcggt
     4501 catcgctggc gagcgtcgga ttcacaatct atctttttgt ggcggccgcg tgcctctctg
     4561 ctgtcggtct ttcgctcgcg ctcctcaccc tcatctttgc gcaagatgca aactgggcct
     4621 ggctcgctct gctggccatt gcggcgttct ggttatcagg gataatcttg atctctgtcg
     4681 gtgaacgtcg ccatcggcgg gctagagagg tttccaagta gcgccgcatg ggcggcgact
     4741 ggcggattac gcctcgttat tccgccctgc ggcttcgaag cggattgacg aatttcagtc
     4801 caatgaaatc cttctcgttg tcggtcacga cgaggcagcc gttggcctca gctacggcgg
     4861 cgatgatcat gtcgagcgca ctccgtggcc gccccttggc cgtaccgtca gccatcagcc
     4921 gcgcccagat gaggcccgcc ttctcgtcga acgggagcac gcggcccgcg aaaagtgctt
     4981 gtggtccatc cggaccggcg aaccaggctt ccaacacagc gcgttttttt cctttgggct
     5041 gttccagcaa gccgcgccgg atttctccaa cggtcagcgc cgagatgaac aggtcactgt
     5101 cagcctgttc cgccatccac gcgagaagag ctgcagatgg agtaggcttc gtgacattgc
     5161 tgatgatgtt ggtgtcgagg agatagcgtg tcacaagtcg accttgcggc cggcttcatg
     5221 cgaacgggcg agactgagat cggccccgac cagcggcgaa cgacgcaacg cggcgaggat
     5281 gccgcccttc tttggtggtt caccggtgat ggtccggctg acggccgctc ggatccgtaa
     5341 cgcatccggg tcgtcctcag caagccgctt ggccagggac cggatcaggt cgcggtcgga
     5401 atccaggcca agaacctcaa atcgcgccat gccgcgcttg accaaccggg tccgatagtt
     5461 ccggagcgct ctcttctgcg ccgtattggc catggccgac tcccaagctt ccgagctatc
     5521 cagaaatata tccagaattt tgcctgttgc aagtgatcgc ccccaaaaac aaaaaaaacg
     5581 gggcatcgct gcctccgttt ttatgccgcg atccgcctgg agcctagccg agccgtcccg
     5641 ccgcgtgggc gagcagggta tagaccatcc cggtctcgga gacgaggtgg ctgcgcagcg
     5701 ccgcgggtcc ccgatcgtcg cgcgcgatct cgtcgagcaa gcgctcgaac tcggtgatgt
     5761 agcggtcgac cgtctgcttg aaggtgcggt cggcgcgata tttgcgggcg acctcgtcga
     5821 aggccttttg gccggccggc gtgtagagcc gcttggtgaa cgccttgctc tcgccgcgct
     5881 gatagcgatc ccacatctcg gatgcgagat tgcggtccat catgcggctg atgtcgagcg
     5941 acagcgactc cagcggattg gcgcccactc ccggcgtctg cggcgcacgc ccgcgcgggg
     6001 cttcgcggtt gctgccgggg acgtcggcgc ggttcaacag gtcggagagc catccatcgc
     6061 gtccctgctc gggattggcc gggctgaccg gcggcgcttc ggtgcggcgc gaagccggcg
     6121 cgccgaggtc cggcggcggc aagctcgatg cgctgctggc ggcttcgcgc gggcgcggcg
     6181 gcgcggtccg caccggcgcc ggcgcctcgc tgcggccggc ggccgccacg gcgtgcatct
     6241 gcatcggctc ttcgtcgcgc ggcgcgctgg tgcggccggc gctgaccacg tccagcccgc
     6301 ggccgtgacg cgcgacgatc cggttcagtt cggccagcgc ctcgatctgg tcgacaatga
     6361 ccttgcgcat ctgcgaggtg ctctcggcgg cttcctgcgg catttccagc acgccgcggc
     6421 gaagttcgtt gcgcgtggct tcgagttcgt tgtgcatttc cagagccatc tgcttcatgc
     6481 tcgagaccat cgcggcgaac ttgtccgccg actgcttgaa catggcgtcg gcttcctgcg
     6541 tggtctgctg atacatttcg ctcatggcgt cggtggtccg ccggcgttca tcttcggccg
     6601 atgcgcgcac cgactcgaac tgccggctga tcgccgcaga acccgcaccg gcggtttccg
     6661 cgaccacccg cgcgatgtcg cgcgcgcgcg attcggcggc ggccagcgat tcatccagca
     6721 agcccgtgaa ccgcgacaac cgatgatcga gatcggcggt tcgcagatcg atcgtcgtca
     6781 ccagcgattc aagctctgcc ttgcgctcgg cgacggagat cgaggtgtcg cggttgcttt
     6841 gctcgaccag accggcggct tcgaccagcg ccttgccgtg gctgtcgaac tggctggaaa
     6901 gcgagccgag gtcctccagc gcccgcgagg tttttgtatt gaagatcgac aactggtctt
     6961 ccagcgtctg ggttgcgatg ccgttgcgcg aggtgacatc gttcatggcg gagacgaagt
     7021 cggccacgcg cgtcaccagg gcgcgctcga gcgaattgag attgtcgtgg gcgccggtga
     7081 gcacctcctg cagcaggatg ttgccttcgc gcaggcgctc gaacaaagcg acggtgtcgg
     7141 ttcgcaggat cttgctggtt tcctgcatct cggtcacagc cgcgatcgac acctggcgcg
     7201 actggtcgat cgccgttcgc gaagcctgct cgaggtcctt cagcgacttg ctcaccgcag
     7261 tggttgcgag ctcgccggcg ttggtaatcg tgcgcgcgac gtcggacccg ctggtcgtca
     7321 tcgattgcga gaacgccagt cccctggttt cgatggattt cagcgaatcc gacgtgacgc
     7381 ggtcgatatc gaccgtcagc tggttggcct tcgcgccgat ggcctcgacc agcgatccac
     7441 gcttgccatc gatcatctgc gcgagacgat cggtctgctg ctggacgtag gtaacgactt
     7501 cgtcggtctt gcccgtcatg gtcgagctga atgtgccgct ggcggcgaga accgagcgtt
     7561 cgacttcagc cgaggtcgac ttgaccttcg aactgacttc gttggatacg gccaccagag
     7621 cgatctgcgc attgagcgca ctggtctgga tattgtcggt ggtactggcg gtgaccgcgc
     7681 cgagcgaccg ctcgatgtcc gcggagatcg ccttgatctg gttcgctgcc tcgcccgacg
     7741 tcgctgtcag cgaaaggtgt gcctcacgcg cactgcccag aattgaagcg gcggtgtcgg
     7801 ccgcgagcga cttgacgtgg ttggcggctt ccgacgatgc ggcgaccagc gtgctctgcg
     7861 cctcgcgtgc cgcggcgacc acggaactgg cggttgcggt gccggcagcc gacagggttc
     7921 gttcgacgtc ggcggccagt gacttgactt ggctcgcagc atccgccgag gccgagacca
     7981 gtgtggtctg ggcctcgcgc gcgccggcga ccaccgaact ggcggtcgcg gtgccggccg
     8041 ccgagagggt ccgttcgacg tcggcggaca acgatttgat ctgggcggcg gcctctccgg
     8101 aggccgtcgt caatgtggtt tgcgcatcgc gggcgctggt caaaatcgag tttgcggcac
     8161 cgctgccgac cgcagtgagg gcgcgctcga cttccgccga ggtcatcttg agctgcgcgc
     8221 cgacgtcgga ggaaaccgtc aacagcgact gctgcgcggc gcgcgcgccg gtctgaatgg
     8281 tctcgctggt gttgaccacc aggtttgtca gcgagcgttc cgcatcctcc acgtgggact
     8341 tgatcccaag cgacaattcc tcggcccgcg ccatcagatc atcgctggcc tggcgtccgc
     8401 tgctctcgat gcggcccgcc acggcttcga cgcgtgaacc cagcagatct tcgaactgcg
     8461 agactcgcat atcgatatcg gtggcaaccg cgccgacgcg ggtttcgata ccctgatgga
     8521 tttcctgaaa ccgcgccgtg atggtgttgg tcagctgggc gctgcgaccg tcaatggttt
     8581 cagtcacatt ggagatgcgc cggtcgaccg cttcgatcgc ctgcgcggtg ccgtcggtca
     8641 gcgaagaggt gagcttggtg aggcgcgagt cgatcgagta gatcgcctgc gc
//
LOCUS       DASWGM010000344         3912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_718979,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000344 DASWGM010000000
VERSION     DASWGM010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718979"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VGA15_09995"
     CDS             <1..1050
                     /locus_tag="VGA15_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9588063.1"
                     /translation="GVLLGNGDRMMPDPILVGRDADKVERLAKKFNVTRWTTDLAKAL
                     LEPSDTIFFDAATTQARPSLLTKAINAGKHVYCEKPIATNLEEAVAVLRLANAKGIKH
                     GTVQDKLFLPGLKKLAFLRDSGFFGRMLSVRGEFGYWVFEGDWQEAQRPSWNYRSEDG
                     GGIILDMVCHWRYVLDNLFGEVESVSCLGTTDIPERWDEKGKKYQATADDSAYATFRL
                     KGGVIAHINMSWVTRVHRDDLVTFQVDGTHGTAVAGLSDCVIQARQATPRPVWNPDEK
                     RTHDFYADWQKLPENSSYDNGFKEQWEMFIRHVCEDAPYKYTLLEGAKGVQLAECALQ
                     SWRERRWIDVAPIKV"
     gene            1062..2255
                     /locus_tag="VGA15_10000"
     CDS             1062..2255
                     /locus_tag="VGA15_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9588064.1"
                     /translation="MNKPVLPASSLSLKLPKADRGIETYRLAASRTFPSKLDGTLNRV
                     AFSAVHVVSDSMADVDPWLNAAVDWDRTIAFREHVWDLGLGVAEAMDTAQRGMGLDWP
                     TSLELIQRSVRAAKARGDALVFSGAGTDHLAVEDARTLDNVIRAYEEQIAAVEKAGGR
                     IILMASRALAKIGRSALDYARVYGRVLSQVREPVIIHWLGDMFDPALANYWGTQNVDQ
                     AMDVAVGIINAHASKVDGVKVSLLDRQREIDMRRRLDRNVKMYTGDDFNYAELIAGDE
                     QGFSHALLGIFDAIAPAASYALSRLAVGDAAGFHDVLGPTVPLSRHIFQAPTRFYKTG
                     IVFMAYLNGHQDHFSMVGGQESTRSTLHLAELFRLADKAGLLANPEQATRRMKAVLAT
                     RGIEP"
     gene            2255..3118
                     /locus_tag="VGA15_10005"
     CDS             2255..3118
                     /locus_tag="VGA15_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX9588065.1"
                     /translation="MRNFSGDHRWLSLNTATVRKQGDLADIIEACARHGIRAIDPWRD
                     QVAAIGLDRAVRAVRDAGLELSGYCRGGMFTSDAAHRMAARDDNRRAIDEAKALGAPC
                     LVLVVGGLPQFSRPGSAASNDITAARAQVHDAIAEMMDYARAVKMPLAIEPLHPAYAA
                     DRACVNTTKQALDLCDALDPKRTGLLGVALDVYHIWWDPELLPQIERAGKDRLLAFHV
                     CDWLVPTKDILNDRGMMGDGVIDIRSIRNAVEAQGFAGYSEIEIFSEGWWEKPMDEVL
                     RTCIERHRSAV"
     gene            3223..>3912
                     /gene="yghU"
                     /locus_tag="VGA15_10010"
     CDS             3223..>3912
                     /gene="yghU"
                     /locus_tag="VGA15_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEX9588066.1"
                     /translation="MTDPSEYTPPKVWSWNKESGGRFASINRPIAGPTHDKELPVGRH
                     PLQLYSLATPNGVKVTVMLEELLALGHRGAEYDAWLIRIDGNQFGSGFVSVNPNSKIP
                     ALMDRSGPTPTRVFESGAILMHLAWKFGALLPTDVAKRAECLSWLFWQIGSAPYLGGG
                     FGHFYAYAPTKIEYAIDRFAMEVKRQLDVLDRQLADNEYVAGDNYTIADIAIWPWYGS
                     LAKGLLYGGGEF"
BASE COUNT          737 a         1176 c         1279 g          720 t
ORIGIN      
        1 ggcgtgctgc tcggcaatgg cgaccgcatg atgccggatc cgatcctggt gggccgcgac
       61 gccgacaagg tcgagcgcct cgccaagaaa ttcaatgtga cgcgctggac caccgacctc
      121 gccaaggcgc tgcttgagcc gagcgacacc atctttttcg atgccgcgac cacccaggcg
      181 cgcccgtcgc tgctgacgaa agcgatcaat gccggcaagc acgtctattg cgagaagccg
      241 atcgcgacca acctcgaaga ggcggtcgcg gtgctcagac ttgccaacgc caagggcatc
      301 aagcacggca cggtgcagga caagctgttc ctgccgggcc tgaagaagct ggccttcctg
      361 cgcgattccg ggttcttcgg ccggatgctc tcggtgcgcg gcgaattcgg ctactgggtg
      421 ttcgaaggcg actggcagga ggcgcagcgg ccgtcatgga actatcgcag cgaagacggc
      481 ggcggcatca ttctcgacat ggtctgccac tggcgctacg tgctcgacaa tctctttggc
      541 gaagtggaaa gcgtgagctg cctcggcact acggatattc ccgagcgctg ggatgagaaa
      601 ggcaaaaagt accaggcgac ggcggacgat tccgcctacg cgaccttccg gctcaagggc
      661 ggcgtgatcg cgcatatcaa tatgtcctgg gtgacgcggg tccatcgcga cgatctcgtc
      721 acctttcagg tcgatggcac ccatggtacg gcggtggccg ggctttccga ttgcgtcatc
      781 caggctcgcc aggcgacgcc gcggccggtg tggaaccccg acgagaagcg cacccatgat
      841 ttctatgccg actggcagaa gctgccggag aacagctctt acgacaacgg ctttaaagag
      901 cagtgggaaa tgtttatccg ccacgtctgc gaggacgcgc cctacaaata cacgctgctg
      961 gaaggcgcca agggcgtgca attggcagaa tgcgcgctgc agagctggcg cgagcggcgc
     1021 tggatcgacg tcgctccgat caaggtgtga ggacgagaac catgaacaaa ccggttttgc
     1081 ctgcctcgtc gctatcgctg aaactgccga aggccgatcg tggaatcgag acctatcgcc
     1141 tcgcggcgtc gcggacgttc ccttcaaaac ttgacggcac gctgaaccgc gtggcgttct
     1201 ccgcggttca cgtggtgtcc gattccatgg ccgatgtcga tccgtggctc aatgccgctg
     1261 ttgattggga ccggacgatc gcctttcgcg aacacgtctg ggatctcggg ctcggcgtcg
     1321 ccgaagcgat ggatactgcg cagcgcggca tgggactgga ctggccgacc tcgctggaat
     1381 tgatccagcg ttcggtgagg gccgcgaagg cgaggggcga tgcgctggtg ttttccgggg
     1441 ccggaaccga tcacctcgcg gtggaggatg ccaggactct cgacaacgtg atccgcgcct
     1501 atgaagagca gatcgcggca gtcgaaaaag ccggcggacg catcatcctg atggcgtcgc
     1561 gggcgctggc aaaaatcggc cgcagcgcgc ttgactatgc cagggtatac ggtcgtgtgc
     1621 tgtcgcaggt ccgcgagccg gtgatcatcc actggctcgg cgatatgttc gatccggcac
     1681 tggcgaatta ctggggcacg caaaacgtcg accaggcgat ggatgtcgcg gtcgggatca
     1741 tcaacgccca cgcgtcgaag gttgacggcg tgaaagtctc gctgctcgac aggcagcgcg
     1801 agatcgacat gcgccgccgt ctcgacagaa acgtaaaaat gtataccggc gacgatttca
     1861 actatgccga actgatcgcc ggcgacgaac agggcttttc gcatgcgctg ctcggcatct
     1921 tcgatgccat cgcgccggcg gcatcctacg cgctgtcgcg gttggcggtc ggcgatgcgg
     1981 ccggtttcca tgacgtgctg gggccgacgg tgccgctgtc gcggcatatc ttccaggcac
     2041 cgacaaggtt ttacaaaacc ggcatcgtgt tcatggcata tctcaacggc catcaggatc
     2101 atttttccat ggtcggcggg caggagagca cgcgctcgac cttgcacctc gccgaactgt
     2161 ttcggctggc ggacaaagct gggttgctcg ccaatcccga gcaggcgacg cggcgcatga
     2221 aagccgtgct cgctacgcgc ggcattgaac cgtgatgcgg aatttttctg gagatcatcg
     2281 ctggctgtcg ttgaataccg cgacggtccg caagcagggt gatctggctg atatcatcga
     2341 ggcctgcgcg cggcatggca tccgcgccat cgatccatgg cgcgaccagg tcgcagccat
     2401 cggcctcgat cgcgccgtgc gtgccgtgcg cgacgccggg cttgaattgt caggctattg
     2461 ccgcggcggg atgttcacct cggacgccgc gcatcgcatg gcggcgcggg acgataatcg
     2521 ccgcgccatc gatgaggcca aggcgctggg cgcgccgtgt ctcgtgctgg tggttggggg
     2581 tttgccgcaa ttttcgcggc cgggaagtgc tgcctcaaac gacatcacgg cagcccgcgc
     2641 gcaggttcac gacgccatcg ccgaaatgat ggactatgcg cgcgctgtaa aaatgccgct
     2701 ggcgatcgaa ccgctgcatc cggcctatgc cgccgaccgc gcctgcgtga acacgaccaa
     2761 acaggcgctc gatctttgcg acgcgctgga tcccaagcgg accggtttgc tcggcgtcgc
     2821 gctcgacgtc tatcacatct ggtgggaccc ggaattgctg ccgcagatcg aacgcgcagg
     2881 aaaggatcgc ctgctggcgt ttcacgtctg cgactggctg gtccccacaa aagacatcct
     2941 taacgaccgc ggcatgatgg gcgacggcgt gatcgatatc agatcgatac gcaacgcagt
     3001 cgaggcgcag ggttttgccg gctattccga aatcgagatt ttttctgaag gctggtggga
     3061 aaagccgatg gatgaagtgc tgcggacctg catcgaacgg catcggtcgg cggtctgagc
     3121 tgtcctcatt ctgcatcgcg gcattgccct tgcgggcctc gtccggtctt gcgatgttcg
     3181 cccgaacatt gaaacatccg ccacaggcct ggagtcgagg caatgaccga cccatccgaa
     3241 tacacgccgc ccaaagtctg gagctggaac aaggagagcg ggggccggtt cgcgagcatc
     3301 aaccgcccga tcgccgggcc gacgcacgac aaggagctgc cggtcggccg ccatcccctg
     3361 cagctttatt cgctggccac accgaacggt gtgaaggtca cggtgatgct ggaggagttg
     3421 ctggcgctcg gccatcgcgg cgcggagtac gatgcgtggc tgatccggat cgacggcaat
     3481 cagttcggca gcggcttcgt gtcggtcaat ccgaactcca agatcccggc gctgatggat
     3541 cgcagcgggc cgacgccgac ccgggtgttc gaatcaggcg cgatcctgat gcatctcgcc
     3601 tggaagttcg gggcgttgct gccgaccgac gtcgcgaagc gcgccgaatg cctgtcgtgg
     3661 ctgttctggc agatcggcag cgcgccctac ctcggcggtg gattcggtca cttctacgcc
     3721 tacgcgccga ccaagatcga atacgcgatc gatcggttcg cgatggaggt gaagcgtcag
     3781 ctcgacgtgc tcgaccggca gcttgcggac aacgagtatg tggcaggcga caactacacg
     3841 atcgcagaca tcgcgatctg gccctggtac ggcagtttgg cgaagggcct gctctatggt
     3901 gggggcgaat tc
//
LOCUS       DASWGM010000345         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_866341,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000345 DASWGM010000000
VERSION     DASWGM010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866341"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1677)
                     /locus_tag="VGA15_10015"
     CDS             complement(19..1677)
                     /locus_tag="VGA15_10015"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX9588067.1"
                     /translation="MSTEELPPRESMEFDVVIVGAGPSGLAAAIRLKQLNADLTVVVV
                     EKGSEVGAHILSGAVIDPVSLDKLIPDWREDADCPLKTQVKADKFYWMTKGMAIPLPA
                     IAMPPLMNNHHCYIGSLGNVCRWLARKAEALGVEIYPGFAATEVLYDEKGAVRGIATG
                     DMGVAKDGSHKSSYTRGMELVGKYTLFAEGARGSLTKQLIAKFSLDAGSEPPKFGIGL
                     KEVWQVDAAKHQKGLIQHSFGWPLNNSTGGGSFLYHYDDNLVAVGFVVHLNYDDPYLS
                     PFDEFQRFKTHPAIRTLFEGGKRLAYGARAITEGGYQSVPRLSFGGGALIGCAAGFVN
                     VPRIKGVHNAVGSGMLAAEHVNTALGSGRANDELVDYENAWRDSPVGKDLYPVRNVKP
                     LWSKFGTVVGVALGGIDMWCNTLGFSLFGTQSHAKPDRKTLDPAKAHAPIAPPKPDGK
                     LTFDKLSSVFLSNTNHEEDQPVHLKVADMNLQKTSEHDVYAGPSNRYCPAGVYEWVEE
                     SAGPRFQINAQNCVHCKTCDVKDPNGNITWVPPEGGGGPNYEAM"
     gene            1823..2641
                     /locus_tag="VGA15_10020"
     CDS             1823..2641
                     /locus_tag="VGA15_10020"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9588068.1"
                     /translation="MTPDSAPTVQQLLAFYLEAGVDCALTEAPVNRLSDPDIVPLSSE
                     TAQPKPVRTIPAAIPATRGEAAPAPEAAILSARDAARTAPSLEVLRALLENFDGCALK
                     STATRLVFADGNPQARIMFVGEAPGREEDIEGLPFVGRSGKLLDRMISAIGLDRSSAY
                     IANVIPWRPPGNRTPTPQETQICLPFIRRQIELVNPDVLVTLGNPSTQTLLSTREGIM
                     KTRGRWFDYDTGTRMIRALATFHPAYLLRSPSYKRMAWLDLRAIAKALENSSNR"
BASE COUNT          503 a          864 c          824 g          518 t
ORIGIN      
        1 cggctcacgc aatcgtggtt acatcgcctc ataattcggt ccgcccccac cctccggcgg
       61 aacccaggtg atattgccgt tcgggtcctt cacgtcgcag gttttgcaat ggacgcaatt
      121 ctgggcgttg atctgaaagc gcggcccggc gctttcctca acccattcat agacgccggc
      181 cgggcagtaa cgattggacg gaccggcata gacgtcatgt tcggatgtct tctgcaggtt
      241 catgtcggcg accttgagat gaaccggctg gtcctcttca tggttggtat tggacaggaa
      301 caccgaggaa agcttgtcga aggtcagctt gccgtccggt ttcgggggcg cgatcggcgc
      361 atgggctttc gccggatcga gcgtcttgcg gtcaggcttg gcatgcgact gggttccgaa
      421 cagcgagaag ccgagcgtgt tgcaccacat gtcgatgccg ccgagtgcca cgccgacaac
      481 ggtgccgaac ttcgaccata gcggcttgac gttgcggacc gggtacagat ccttgccgac
      541 gggagaatcg cgccaggcat tctcataatc gaccagttca tcgttggcac ggcccgagcc
      601 cagcgcggta ttgacgtgtt cggcagccag catgccgctg ccaacggcat tgtggacgcc
      661 cttgatgcgc ggcacattga caaagcccgc cgcgcagccg atcagcgcgc cgcccccaaa
      721 gctcaggcgc ggcaccgatt gataaccgcc ctcggtgatg gcgcgcgcgc cataagcgag
      781 ccgcttgccg ccttcgaata atgtgcggat cgcgggatgg gtcttgaagc gctggaattc
      841 atcgaacggc gacagatacg gatcgtcata gttgagatgc acgacaaaac cgaccgcgac
      901 cagattgtcg tcgtagtggt acaggaacga gccgccgccg gtcgaattgt tcaacggcca
      961 gccgaacgaa tgctggatca gacctttttg atgcttggcg gcgtcgacct gccagacctc
     1021 cttgaggccg atgccgaatt tcggcggctc gctgccggca tcgagggaaa attttgcgat
     1081 cagctgtttg gtcaggctgc cgcgcgcgcc ctcggcgaac agcgtgtatt tgccgaccag
     1141 ctccatgccg cgcgtgtacg acgatttgtg cgagccgtcc ttggcgacgc ccatgtcgcc
     1201 ggtggcgatg ccgcgcaccg cgcccttttc atcgtacagc acttcggtgg cggcaaagcc
     1261 cggatagatc tcgacgccga gcgcctcggc cttgcgcgca agccagcggc acacattgcc
     1321 gagcgagccg atatagcaat ggtgattgtt catcagcggc ggcatcgcga tcgccggcag
     1381 cggaatcgcc atgcccttgg tcatccagta gaacttgtcg gccttgacct gggtcttcag
     1441 cggacagtcg gcgtcctcgc gccaatccgg aatcaatttg tcgagcgaca ccggatcgat
     1501 caccgccccg gacaagatat gcgcgccgac ttccgacccc ttctccacca cgacgaccgt
     1561 cagatcggcg ttgagctgct tgagccggat cgcggcggca aggcccgagg gtccggcgcc
     1621 gacgatcacg acgtcgaatt ccatggattc gcgcggcggc aattcttcgg tgctcatgat
     1681 ctgatctcag cccggttgag aaagcgatga taagtcgttt gtttccgatt tttcgcctga
     1741 gaacaaccac ggaaatgcgc gttgagacgt ttcgctgcag cgcaatccat cctagattgg
     1801 cctgatgccc caacatggaa ccatgacgcc tgatagcgcc cccactgttc agcaattgct
     1861 ggccttttat ctggaggccg gagtcgactg tgcgttgacg gaggcgccgg tcaaccggct
     1921 gtccgatccc gacatcgttc ccctatcaag cgaaaccgcg cagcccaaac cggtcaggac
     1981 cattcctgcc gcgatcccgg cgacacgcgg cgaggcagcg ccagcgccag aggccgcgat
     2041 cctgtcggcg cgggacgcgg cgcgaacggc accttcgctc gaggtgctgc gcgccctgct
     2101 ggagaatttc gatggctgcg cgctgaagtc caccgcgacg cgcctggtat tcgccgacgg
     2161 caacccgcag gcgcgcatca tgtttgtcgg cgaagccccc gggcgcgagg aggacatcga
     2221 ggggctgccg ttcgtcggac gttccggcaa gctgctggac cggatgatat cagccatcgg
     2281 gctcgaccgt tccagcgcct acatcgccaa tgtcattcca tggcggccgc cgggcaaccg
     2341 cacgccgacg ccgcaggaga cgcaaatctg cctgccgttc atccggcggc aaatcgaact
     2401 ggtgaatccg gatgtgctgg tcacgctcgg caatccctcg acccagaccc tgctgtcgac
     2461 gcgcgagggc atcatgaaaa cccgtgggcg ttggttcgac tatgacaccg gcacgcggat
     2521 gatccgcgcg cttgccacct tccatccggc gtatttgctg cgctcgccat cctacaagcg
     2581 catggcgtgg ctggatttgc gcgcgatcgc caaggcgctg gagaattcct ccaatcgatg
     2641 aggcatcggt tctacatcac ggcaacggaa gcgcgctccc tctccaccca actcgggtct
     2701 acccgagtt
//
LOCUS       DASWGM010000346         5302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2393130,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000346 DASWGM010000000
VERSION     DASWGM010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393130"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VGA15_10025"
     CDS             complement(<1..656)
                     /locus_tag="VGA15_10025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX9588069.1"
                     /translation="MAEFWTSTLWPLIIMIAQSVLLLVVLLIAIAYILLADRKIWAAV
                     QIRRGPNVVGPWGLLQSFADLLKFVLKEPIIPSGANKGVFLLAPLVSCVLALAAWAVI
                     PMDLGWVISDINVGVLYIFAISSLSIYGIIMAGWSSNSKYPFLAALRSAAQMVSYEVS
                     IGFVIITVLLCVGSLNLSTVVEAQNTRGLASLIGLPWLTFLNWYWLPLFPMFVVFYVS
                     A"
     gene            complement(667..2742)
                     /gene="nuoG"
                     /locus_tag="VGA15_10030"
     CDS             complement(667..2742)
                     /gene="nuoG"
                     /locus_tag="VGA15_10030"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX9588070.1"
                     /translation="MTKLIVDGKEIDVPAEFTLLQACEAAGAEIPRFCYHERLSIAGN
                     CRMCLVEVKGGPKPVASCAWAVRDCRPGPNGEPPEISTRSPMVKKAREGVMEFLLINH
                     PLDCPICDQGGECDLQDQAMGYGVDTSRFAENKRAVEDKYLGALVKTSMNRCIQCTRC
                     VRFAAEVCGVPEMGATGRGEDMEITTYLESALTSELQGNLVDICPVGALTSKPYAFAA
                     RPWELGKTQSIDVMDGVGSAIRVDTRGREVMRILPRVNEAVNEEWISDKTRHIVDGLR
                     TQRLDRPYIRENGQLRAASWSEAFGAIAAKAGRIDGKRIGAIAGDLAAVEEMFALKEL
                     LAKCGSANLAVQGGDAFNPKAGRASYIFNPGIAGIEQADALLIVGSNPRKEAAVLNAR
                     IRKRWRSGQLKIGVIGAKADLTYDYVHLGAGTDSLSALAAGKHSFADVLKAAKNPIVL
                     VGAGAAARHDGAAILALAAKLALDFGAVKDGWNGFGVLQDTASRVGALDIGFAGGAGG
                     LSAAQMTTFGTLDVLFLLGADEIKVPDGTFVVYIGTHGDRGAHRADVILPGAAYTEKS
                     GIYVNTEGRVQMASRASFPPGEAREDWAIIRALSDVLGKKLPYDSLGALRQALFKAAP
                     HLMRVDQIEAGKAADINTLAAKGGTLEKTPFKTSVEDFYLTNPIARASAVMAECSRLA
                     SGQMLTAAE"
     gene            complement(2750..3241)
                     /locus_tag="VGA15_10035"
     CDS             complement(2750..3241)
                     /locus_tag="VGA15_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588071.1"
                     /translation="MSQPSISWWQKYGTLAQMAQAAVALLGFMAILFQINEIRSNNRA
                     ASARQAFLGYTDLAFRNPKFSAPDYDAIKVSGRDEQVQYESFVSYFLYACEEATAAFA
                     DRREWLASCDYDLKPHLPFLCEKNKAEPAYLATYSADTQQWVKASMKTASVTPPDCKL
                     GKT"
     gene            complement(3247..4572)
                     /gene="nuoF"
                     /locus_tag="VGA15_10040"
     CDS             complement(3247..4572)
                     /gene="nuoF"
                     /locus_tag="VGA15_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX9588072.1"
                     /translation="MLDDKDRIFRNLYGFQDWGLEAARRRGAWDGTKAIIDKGRDWII
                     NEMKASGLRGRGGAGFPTGLKWSFMPKESTDGRPNYLVVNADESEPGTCKDREIMRHD
                     PHLLVEGCLLASFAMGAHACYIYVRGEFIREREHLEAAVAQAYEAKLIGKDNINGFPF
                     DLYVTHGAGAYICGEETALLESLEGKKGMPRLKPPFPANVGLYGCPTTVNNVESIAVA
                     PDILRRGAAWFAAIGRPNNVGTKLFCISGHVERPCNVEEAMGIPFRELIERHCGGVRG
                     GWDNLKAVIPGGSSVRMVPADQIIDTPMDFDSLGKLRSGLGTAAVIVMDKSTDLIRAI
                     ARISYFYKHESCGQCTPCREGTGWMWRVLTRMAEGRAHKREIDMLLEVTKQIEGHTIC
                     ALGDAAAWPIQGLIAHFRHEIEERIDQYSHKADVDDAGILDPAHMVAAE"
     gene            complement(4594..4809)
                     /locus_tag="VGA15_10045"
     CDS             complement(4594..4809)
                     /locus_tag="VGA15_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588073.1"
                     /translation="MKRDRILEAVGSGSVGAMKNWRYIALHTAAAAAFIFLLQRYALN
                     ASLDSSLLWALTIGGCAAGLAYAQSNR"
     gene            complement(4806..>5302)
                     /gene="nuoE"
                     /locus_tag="VGA15_10050"
     CDS             complement(4806..>5302)
                     /gene="nuoE"
                     /locus_tag="VGA15_10050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX9588074.1"
                     /translation="SAVIPILWRVQEQHEGWVSEAAIRAVADLLDMPYIRALEIATFY
                     TMFQLQPVGKKAHVQVCGTTPCRLRGAGDIIEVCQSRIHHDPFRLSKDGNFSWEEVEC
                     LGACVNAPMVLIWKDTYEDLTKENFGKVLDGFASGNLPKPGPQVDRQFSAPVGGPTTL
                     KEEA"
BASE COUNT          999 a         1706 c         1638 g          959 t
ORIGIN      
        1 gccgagacat agaacaccac gaacatcggg aacagcggca gccagtacca gttcaggaac
       61 gtcagccacg gcaatccgat caggcttgca agaccacgcg tgttctgcgc ctcgaccacg
      121 gtcgagaggt tcagcgatcc cacgcacagc aagacggtaa tgatcacaaa gccgatcgag
      181 acttcgtaac tcaccatctg cgccgccgag cgaagggcgg cgagaaacgg atatttcgaa
      241 ttcgacgacc agccggccat gatgatgccg tagatcgaca gcgacgagat tgcgaagatg
      301 tagagcacgc cgacattgat gtcggagatc acccagccga gatccatcgg aatcacggcc
      361 caggccgcca gcgccagcac gcaacttacc agcggcgcca gcaggaacac gcccttgttg
      421 gcgccggagg gaatgatcgg ctccttcagc acgaacttca acaggtcggc gaaggattgt
      481 aacaggcccc aggggccgac gacgttgggt ccgcgccgga tctgcacggc ggcccagatc
      541 ttgcggtcgg ccagcaggat gtaggcgatc gcgatcagaa gcaccacgag cagcaggacg
      601 ctctgcgcga tcatgatgat cagcggccac aacgtgctgg tccagaattc agccatcagg
      661 tcacgctcac tccgctgccg tcagcatttg cccggaggcc aggcgggaac attccgccat
      721 caccgccgac gcgcgcgcga tcgggttggt caggtaaaag tcttcaaccg aagtcttgaa
      781 cggcgtcttc tcgagggtac cgcctttggc cgcaagcgta ttgatgtcgg ccgctttgcc
      841 ggcttcgatc tgatcgacgc gcatcagatg aggtgctgcc ttgaacaggg cctgccgcag
      901 cgcgccgaga gaatcgtaag gcagtttctt gcccagaaca tccgaaagcg cgcgaatgat
      961 cgcccaatcc tcacgcgcct ctcccggcgg gaacgaggcg cggctggcca tctgcacccg
     1021 gccctcggtg ttgacataga tgccggattt ctcggtatag gccgcaccgg gcaggatgac
     1081 gtcggcgcga tgggcgccgc ggtcgccatg ggtgccgatg taaaccacga aggtgccgtc
     1141 cggcaccttg atctcgtcgg cgccaagcag gaacaagaca tcaagcgtgc cgaatgtcgt
     1201 catctgcgcc gcgctgaggc cgcccgcgcc cccggcaaag ccgatatcga gcgcgccgac
     1261 ccgcgaggcg gtatcctgca gaacgccgaa accgttccag ccgtccttga ccgcgccgaa
     1321 atccagcgcc agcttcgccg ccagcgccaa tatcgcagca ccgtcatgcc gcgcggccgc
     1381 accggcgcca accagcacga tcggattttt cgcagccttg agcacgtccg cgaaggaatg
     1441 cttgccggcg gcgagagcgc tgagcgaatc cgtgcccgcg ccgagatgga cgtagtcata
     1501 ggtgagatcg gccttggcgc cgatcacgcc gatcttgagc tggccgctgc gccagcgctt
     1561 gcggatgcgc gcgttgagaa cggcggcctc cttgcgcgga ttcgagccga cgatcagcaa
     1621 agcgtcggcc tgctcgatcc cggcaatacc aggattgaag atataggagg cgcgaccggc
     1681 cttcgggttg aaggcgtcgc cgccctggac cgcgagattg gctgagccgc atttcgccag
     1741 cagttccttc aacgcgaaca tctcttccac cgcggcgaga tcaccggcga tggcgccgat
     1801 ccgcttgccg tcgatgcggc cggccttcgc cgcgatggcc ccgaatgctt ccgaccatga
     1861 cgccgcacgg agctgaccgt tctccctgat gtagggacgg tcgagccgtt gcgtgcgcag
     1921 gccatcgacg atgtggcggg tcttgtcgga aatccattcc tcgttcacgg cctcgttgac
     1981 gcgcggcaga atccgcatca cctcgcggcc gcgggtatcg acccggatcg ccgaaccgac
     2041 gccgtccatc acatcgatcg actgggtctt gccgagctcc cagggccgcg ccgcgaacgc
     2101 atagggtttt gaggtcagcg cgcccaccgg gcagatgtcg acgagattgc cctgcagttc
     2161 cgaagtcagc gcggactcca gataggtggt gatttccatg tcctcgccgc ggccggtcgc
     2221 gcccatttcg ggcacaccgc agacttcggc ggcgaagcgg acgcagcgcg tgcactggat
     2281 gcagcggttc atcgaggtct tgaccagcgc gcccagatat ttgtcctcga ccgcgcgctt
     2341 gttctcggcg aagcggctgg tgtcgacgcc gtagcccatc gcctgatcct gcaggtcgca
     2401 ctcgccgccc tggtcgcaaa tcgggcaatc cagcggatgg ttgatcagca ggaattccat
     2461 cacgccttct cgggccttct tcaccatcgg cgagcgcgtc gagatttccg gcggttcgcc
     2521 gttggggccg ggacggcaat cgcgaaccgc ccaggcgcag ctcgcaaccg gtttcgggcc
     2581 gcccttcact tcgacaaggc acatccggca attgccggcg atcgacagcc gttcgtggta
     2641 gcaaaaccgc ggaatttccg cgcccgcggc ctcgcacgcc tgcagcagcg tgaattccgc
     2701 ggggacatcg atctctttgc catcgacgat gagtttggtc atagctttct caagtctttc
     2761 cgagcttgca gtcgggtggt gtgacgctgg cggtcttcat ggaagccttc acccattgct
     2821 gggtgtcggc gctgtaggtc gcgagatagg caggctcggc cttgttcttc tcgcacagga
     2881 atggcagatg cggcttcagg tcgtaatcgc aggaagccag ccattcgcgc cggtcggcga
     2941 atgcggcggt cgcttcctcg caggcataga ggaaatagga gacaaaactc tcatattgga
     3001 cctgctcgtc gcggccgctg accttgatcg cgtcgtagtc cggcgccgag aatttcggat
     3061 ttctgaacgc cagatcggta tagcccaaaa acgcctgccg tgcactggcc gcccgattgt
     3121 tggagcggat ttcgttgatc tgaaacagga tagccataaa gccgagcagc gccacggcag
     3181 cctgcgccat ctgcgccagc gtcccgtatt tctgccacca ggagatgctc ggttgcgaca
     3241 tcgatatcac tccgccgcga ccatatgcgc ggggtccaga atgccggcat catcgacatc
     3301 cgctttatgc gaatattgat cgatgcgttc ctcgatctcg tgacggaaat gcgcgatcag
     3361 gccctggatc ggccacgccg cggcgtcgcc gagcgcgcag atggtgtggc cctcgatctg
     3421 tttggtcact tccagcagca tgtcgatctc gcgcttgtgg gcgcggcctt cggccatgcg
     3481 ggtgagcacg cgccacatcc acccggtgcc ctcgcggcac ggcgtgcact ggccgcagct
     3541 ctcgtgcttg tagaaatagg aaatccgcgc gatcgcccgg atcagatcgg tcgacttgtc
     3601 catcacgatg acagcggcgg tgccgagacc ggagcgcagt ttgcccaggc tgtcgaaatc
     3661 catcggggta tcgatgatct ggtcggccgg caccatgcgc accgaggagc cgccggggat
     3721 cacggctttg agattgtccc agccgccgcg gacaccgccg caatggcgct cgatcagttc
     3781 gcggaacgga atccccatgg cttcttcgac attgcaggga cgctcgacat ggccggaaat
     3841 gcaaaacagt ttggtgccga cattgttggg ccggccgatg gcggcgaacc aggccgcacc
     3901 ccgccgcaga atatccggcg cgaccgcgat cgactcgaca ttgttgaccg tggtcgggca
     3961 gccgtagaga ccgacattgg ccgggaatgg cggtttgagg cgcggcatgc ctttcttgcc
     4021 ttccaggctt tcgagcagcg cggtctcctc gccgcagata taggcgccgg cgccgtgggt
     4081 cacgtaaaga tcgaacggaa aaccgttgat attgtccttg ccgatcagct tggcctcata
     4141 ggcctgagcg acagcggcct ccagatgctc gcgctcccgg atgaattcgc cgcgcacgta
     4201 aatgtagcag gcgtgcgcgc ccatcgcgaa gctcgcgagc aggcagccct cgaccagaag
     4261 atgcggatcg tgccgcatga tctcacggtc cttgcaggtg ccgggctcgg actcgtcggc
     4321 attgaccacg aggtaattcg gccggccgtc ggtcgattcc ttcggcatga acgaccattt
     4381 caggccggtc ggaaaaccgg ccccgccgcg gccgcgcagc cccgaggctt tcatttcgtt
     4441 gatgatccag tcgcgaccct tgtcgatgat cgccttggtg ccgtcccagg cgccgcggcg
     4501 gcgcgcagct tcgaggcccc agtcctggaa gccgtagagg ttgcggaaga tgcggtcctt
     4561 gtcatcgagc atgacgtgaa gcttccctag ggatcaacga ttggactgcg cataagcgag
     4621 cccggcggcg catccgccga tggtcagcgc ccatagcaga cttgaatcaa gcgacgcatt
     4681 cagcgcgtag cgctgcagca gaaaaatgaa tgcagcggcg gctgccgtgt gcaaagcgat
     4741 atagcgccag ttcttcatcg caccgacact cccgctgccc accgcttcca atatgcggtc
     4801 gcgcttcatg cttcttcctt caacgtggtc ggtccaccca ccggcgcgga aaactgacgg
     4861 tcgacttgcg gtcccggctt cggaagattg ccggacgcaa agccgtccag caccttgcca
     4921 aaattttcct tggtcagatc ctcgtaggta tccttccaga tcagcaccat cggcgcgttc
     4981 acgcaggcgc caaggcactc gacttcttcc cagctgaaat tgccgtcctt ggagagacga
     5041 aagggatcgt gatggatgcg gctctggcac acctcgatga tgtcgccggc gccgcgcagc
     5101 cggcacggcg tggtgccgca gacttggacg tgcgccttct tgccgaccgg ctgcagctgg
     5161 aacatcgtgt agaaggtcgc gatctccagc gcgcggatgt agggcatgtc cagcaaatcc
     5221 gcgaccgcgc ggatcgccgc ttccgaaacc catccctcat gctgttcctg gacccgccac
     5281 aggatcggga tcaccgcgga cg
//
LOCUS       DASWGM010000347         5223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2658770,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000347 DASWGM010000000
VERSION     DASWGM010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658770"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1077)
                     /locus_tag="VGA15_10055"
     CDS             complement(292..1077)
                     /locus_tag="VGA15_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9588075.1"
                     /translation="MLGNDQIVAASRTLHDHWRAGTKLQGLEATQRPRDRAEGYAIQA
                     RIENYTTKTLFGWKIAATSEAGQRHINVDGPMAGRILPETVIPDGGTASMAGNEMRVA
                     EPEFAFRMHADLPPRPSAYTVQEVVDAVDTLHPAIEIPDSRFRDFVNAGAAQIIADNA
                     CAHLFVLGAPTTSNWRALDLVEERPVITLRDRQFTGHGKNVLGDPRVALTWLANELRG
                     LGVTLRAGQVVTTGTCHPPLPIQSGDVVEADFGLIGRVSVVFK"
     gene            complement(1100..1669)
                     /locus_tag="VGA15_10060"
     CDS             complement(1100..1669)
                     /locus_tag="VGA15_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9588076.1"
                     /translation="MGIPDISITYQRTVRDRPLSAVRIRAAAGDPRRGWLTADGQAIP
                     VALGRGGIRANKREGDGGTPRGTFRPRQLWWRADRHPRPCTFLPIRAIRPQDAWCEDP
                     ASRHYNRPVRLERKSGGDRLTRDDHLYDFIVEIDHNSSPRIAGRGSAVFLHLARDNFG
                     PTAGCVSMTKSAMLRLLRRLGPETRIVIG"
     gene            1894..2580
                     /locus_tag="VGA15_10065"
     CDS             1894..2580
                     /locus_tag="VGA15_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9588077.1"
                     /translation="MANARKILIVDDDTDLRDTLVEQLSLHDEFEASAVDTGAKGASA
                     AKADAPDLVLMDVGLPDTDGREVVRSLRKGGFKAPIIMLTGHDTDSDTILGLESGAND
                     YVAKPFRFAVLLARIRAQLRQHEASEDAVFSVGPYSFRPGSKMLTGANARKVRLTEKE
                     TAILRFLYRAGQLPVSRETLLQEVWGYNSGVTTHTLETHIYRLRQKIEKDAANPEILV
                     TEAGGYKLVP"
     gene            2788..3231
                     /locus_tag="VGA15_10070"
     CDS             2788..3231
                     /locus_tag="VGA15_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9588078.1"
                     /translation="MSIEDDVALLERVPTLHLLGTAALRMLAIGSEQRDVARGDLLFN
                     EGDDADAGFIVQRGALRVEDSSGAEIVVGPGALIGELALVVAMQRPSTVTALEHCSVI
                     RITRSLFQRVLESDPAAAQRLRDEFTSRTSQIASDILMAGAKLSI"
     gene            complement(3451..4266)
                     /locus_tag="VGA15_10075"
     CDS             complement(3451..4266)
                     /locus_tag="VGA15_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9588079.1"
                     /translation="MRFSLTTWNINSVRLRIDLVAKFLKSVRPDVLCLQETKCVDDAF
                     PLKRFRRLGYEHVALNGQKGYHGVAVISKLPFETTDIRTFCDKIDSRHISVQFGEKAQ
                     LSKPLVLHNFYVPAGGDIADPALNPKFEHKLRFLDEMKACEPLHPRGDDRHILVGDLN
                     VAPHENDVWSHKQLLKVVSHTPIECEKLLAAQTHGEWIDVARERIPLSEKVYTWWSYR
                     AADWTLADRGRRLDHIWVSRALKERVSDFRITRDARGWERPSDHVPVTVTLEV"
     gene            complement(4360..5205)
                     /locus_tag="VGA15_10080"
     CDS             complement(4360..5205)
                     /locus_tag="VGA15_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX9588080.1"
                     /translation="MARDFVVPGLRTALALLIAAAVASVSTPSAAQLFSTTPSPPAVA
                     VPMPKPAPKARRLQMTASDPQKAQMTTGATPAAPPNPVIPDPHRNIPANLFATFDANQ
                     KAQAAKVSSYLSSLQTLVGNFVQVGPDGSKTKGDFYIQKPGKVRFEYDDPSPIAIIAD
                     GSSLAVRDRKLATQDIYPLSQTPLRFLLSDRIDLLKDTNVVSVTSDEVYISVTIEEKQ
                     ALIGTSRLMLMIGTKDGQLKQWTVTDPQGYDTTVAVYNLDANKKVDPGLFKIDFTNYP
                     TTPPG"
BASE COUNT          981 a         1581 c         1602 g         1059 t
ORIGIN      
        1 cggcctagca tgggggaggt ttggcaagat cgcggtcatt cggcgccggc tcaaccgaaa
       61 aactttgcgg tcgccgccct caagcccatc aaatgcaaaa ttctccgggc gagagcaaaa
      121 cattctcacg ctctgaggag cgcgctcttg cgcgcgtctc gaaggatggg ccgcataccc
      181 cgctcatcat ccttcgaggt tcgctcaacg gcgcaaggcg ccgtcgggct cgcacctcca
      241 gcgatgagcg caattgcgct cacgcgggga tgacggcatc atttgggtct cctacttgaa
      301 cacgaccgac accctgccga tcaaaccgaa atccgcctcg accacatcgc ccgactgaat
      361 cggcagcggc gggtggcagg tgcctgtggt cacgacctgg cccgctctca gcgtcacgcc
      421 aagcccgcgc aattcattgg cgagccacgt caaggcaacc ctggggtcgc cgagcacgtt
      481 cttgccatga ccggtgaatt gcctatcccg cagcgtgatg accggcctct cctcgacgag
      541 atcgagcgct cgccagttcg acgtcgttgg cgcaccaagt acgaacaggt gcgcgcatgc
      601 attgtccgcg atgatttgcg cggcgccggc gttgacgaaa tccctaaaac gcgaatcggg
      661 aatctcgatc gccggatgaa gtgtgtcgac cgcatcgaca acctcttgta cggtgtaggc
      721 tgacggtcgc ggcggcaagt ccgcatgcat gcgaaaggcg aattcgggct cggcgacgcg
      781 catttcattt cccgccatgg aagcggtgcc gccatccgga atgaccgttt cgggcaggat
      841 gcgtcccgcc atcgggccgt cgacattgat atgcctttgc cccgcttcgc tggtggctgc
      901 gattttccag ccgaacagag ttttggtcgt gtaattttcg attctcgcct ggatcgcgta
      961 accttcggcg cggtcgcgcg gccgctgcgt cgcttcgaga ccttgaagct tggtgccggc
     1021 gcgccagtga tcgtgcaagg tgcgtgatgc ggccactatc tgatcattgc caagcattat
     1081 tcttgtcccg gaatggtgtt catccaatca cgattctggt ctcgggaccg agtcgtcgca
     1141 gtaagcgcag catggcggat tttgtcatcg agacgcatcc tgcggtcggc ccaaaattgt
     1201 cgcgcgccaa gtgcaagaat accgcgctgc cgcggccggc aatgcgcggc gagctgttgt
     1261 gatcgatctc gacgatgaaa tcataaagat ggtcgtcgcg cgtcagccga tcgccgccgc
     1321 tcttgcgttc cagccgaacc ggccggttgt aatggcggct cgctggatct tcgcaccagg
     1381 catcttgggg ccgaatggcg cgaatcggca ggaatgtgca tggccgcggg tggcggtcgg
     1441 cgcgccacca taattgccgc ggtcggaacg ttcccctcgg ggttccgcca tccccttccc
     1501 gcttgttggc cctgatgccg ccgcgtccaa gcgcgaccgg tatggcctgc ccgtctgcgg
     1561 taagccatcc gcggcgtgga tccccggcgg cggcgcgaat cctgacggcg gaaagcggtc
     1621 gatcacgcac ggttctctga taagtgattg aaatgtcggg aattcccatg cgatacgcga
     1681 atctctatgg tttgacgcta gctctaaatg ttctatttca cgccattaca ggacattcat
     1741 ccaaaacccc gggaattctg gagtacactg gtgcaacttg tgaatctgta acagccccct
     1801 tacctcaata tgaatcaaga ccaaccgacc acgattttac cgaccaaaac tcgactgata
     1861 gcgctccgcg cctgccccca gaggattgta cccatggcca atgcccgcaa gatcctgatc
     1921 gtggacgacg ataccgatct gcgcgatacg ctggtggagc aattgtcgct gcatgacgaa
     1981 ttcgaagcct cggcggtcga taccggcgcc aaaggtgcca gcgccgccaa ggccgatgct
     2041 cccgatcttg tgctgatgga tgtcggactg cccgataccg atggccgcga agttgtgcgc
     2101 agcctgcgca agggcggctt caaggctccc atcatcatgc tcaccggaca cgataccgat
     2161 tcggacacga tcctcgggct cgaatccggc gccaacgact acgtcgcaaa gccgtttcgc
     2221 ttcgccgtgc tgctggcgcg gatccgggcg cagctccgcc agcatgaagc cagcgaagac
     2281 gcggtgttct ccgtcggtcc ttacagtttt cgacccggat ccaagatgct gaccggcgcc
     2341 aacgcccgga aggtgcgcct gaccgaaaag gaaacggcga tcctgcggtt tctgtaccgg
     2401 gccggccagc tgccggtatc gcgcgagacg ctgctgcagg aagtctgggg ctataattca
     2461 ggcgttacca cccacaccct ggaaacccac atttaccggc tgcgtcaaaa aatcgagaaa
     2521 gatgcagcca atcccgagat attggtaacg gaagccgggg gctacaagct ggtaccttga
     2581 tactattgat atggtgcttt gaatcgcctg gtgcggcggc tattgcctgg aagagtttcg
     2641 gtctctgaag acaccgtcaa tcgatgattt cactgcgatt ttggttcaga agttttctcg
     2701 acgttgtctt gaagctttcc tggaagactt gcggcggaag cgaagcgaac ttctgcaccg
     2761 ccgcgttagc ccgatcaccg gttctgcatg tcgatcgaag atgatgttgc cctgctcgaa
     2821 cgcgtcccga cattgcacct gttgggaacg gccgcgttgc gcatgctggc gatcggctcc
     2881 gagcagcgcg atgtcgcgcg gggcgatctg cttttcaatg aaggcgacga tgcggacgcg
     2941 ggatttatcg ttcagcgcgg cgcgctccgg gttgaggata gcagtggcgc cgagatcgtc
     3001 gtcggcccag gcgcattgat cggcgagctt gcgcttgtcg tcgcgatgca gcggccctcc
     3061 acggtaacgg cgctggaaca ctgctcggtg atccgcatca cgcgcagcct gtttcagcgc
     3121 gtgctggaaa gcgaccccgc cgccgcgcaa cggctgcgcg acgaattcac cagccgcacc
     3181 agccagatcg ccagcgatat cctgatggcc ggcgcgaagc tgagtatttg agccctcatg
     3241 accacgccct ccgggagtcg tcccggacaa gtgagccaac gggtccggcc tttggccggc
     3301 ccgatgacag gctccgcgaa cgcgatccgg gatccatacg ccgtgttgtc tcgtttgggc
     3361 cgtgggtcag atgccttctg caacaatcaa cgcggcggtt atgggtcccg gcgttcgccg
     3421 ggacgacccg tggaaaggcg gtctgtaaac tcacacctcc agcgtcactg tcaccggcac
     3481 gtggtcggac ggccgttccc agccgcgcgc atcccgcgtg atccggaaat cgctgacacg
     3541 ctctttgagg gcgcgcgaca cccagatgtg atcgagccgg cggccgcgat cggccagcgt
     3601 ccagtctgcg gcgcgataac tccaccacgt atagactttt tccgagagcg ggatgcgctc
     3661 gcgcgcgaca tcgatccatt cgccgtgagt ctgcgccgcc agcagtttct cgcattcgat
     3721 cggggtgtgc gacacgactt tcagtaattg cttgtgcgac caaacgtcat tctcatgcgg
     3781 cgccacattg agatcgccga ccaggatatg gcggtcatcg ccgcgcgggt gcagcggctc
     3841 gcacgccttc atctcgtcga ggaatcggag cttgtgctcg aacttcggat tgagggcggg
     3901 atccgcgata tcgccgccgg ccggaacata gaaattatgc agcaccagcg gctttgacag
     3961 ctgcgccttt tcgccgaact gaaccgagat gtggcgggaa tcgatcttgt cgcagaaggt
     4021 gcggatgtcc gtggtctcga acggcagttt cgagatcacg gccacgccgt gatagccctt
     4081 ctgcccgttc agcgcgacgt gctcatagcc gaggcgcctg aaccgcttca gcggaaatgc
     4141 gtcatcgacg catttggttt cctgcaggca cagcacgtcc ggcctcaccg acttgaggaa
     4201 tttggcgacg aggtcgatgc gcaggcgcac cgaattgata ttccacgttg tcaaggagaa
     4261 gcgcatctct gctggggggc cttgcgacgc gaggatacgc tcatccggcg atgacgctgt
     4321 ccactgtgaa gcgcgatcag gagcaatcaa gaatggtttt cagcccggtg gcgtggtcgg
     4381 gtagttggtg aagtcgatct tgaacaggcc gggatcgact tttttgttgg cgtccaggtt
     4441 gtagaccgcc acggtggtgt catagccttg cggatcggtg accgtccatt gcttgagctg
     4501 accgtccttg gtgccgatca tcaacatcag acggctggtg ccgatcagcg cctgcttctc
     4561 ctcgatggtg acgctgatgt agacctcatc cgaggtcacg ctcacgacgt tggtgtcctt
     4621 caagagatca atgcgatccg atagcagaaa ccgcagcggc gtctgcgaca gcggatagat
     4681 gtcctgggtc gcgagcttgc ggtcgcgcac cgccagcgac gatccgtcgg cgataatggc
     4741 gatcgggctc gggtcgtcgt attcgaaacg caccttgccg ggcttctgga tgtaaaaatc
     4801 gcccttggtc ttgctgccgt cgggcccgac ctggacgaaa tttccgacca gcgtttgcag
     4861 cgacgacaga taggagctca ccttggcggc ttgcgccttt tgattcgcat cgaaggtcgc
     4921 aaaaagattg gcgggaatat tcctgtgcgg atcgggaatc accggattcg gcggggcagc
     4981 cggggtcgcc ccggtcgtca tctgggcctt ctgcgggtcc gacgcggtca tttggagacg
     5041 gcgggccttt ggcgccggtt ttggcatggg cacggccacg gccggcggag atggcgtcgt
     5101 cgagaagagc tgcgcagcag acggtgtcga cacgctcgcc acggccgccg cgatcagcaa
     5161 cgctaaagcc gtgcggaggc cgggaaccac gaagtctctt gccattcgaa ttttcggaat
     5221 ctg
//
LOCUS       DASWGM010000348         3746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2112517,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000348 DASWGM010000000
VERSION     DASWGM010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112517"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..>3746)
                     /locus_tag="VGA15_10085"
     CDS             complement(38..>3746)
                     /locus_tag="VGA15_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9588081.1"
                     /translation="YYGPVYFRRESEPYMTIALAGTRKDAGVSIAEVNLKLIWDVVSQ
                     IKVGERGHAYVVGAEGRLIAHPDISLVLRNTDMSKLVQVQAAQSGSTDAEALQGALNI
                     QGQEVLTASAPIAPLGWTMFVELPVKEAYASLYAALQRLATVLAAALIFAVLAGIFLA
                     RRMVGPIQALRAGAERIGGGDFAQRISIRTGDELEGLANQFNDMGARLQESYADLEKK
                     VETRTAELTESLEQQTATSEVLEVISSSPGELEPVFQKMLENATRVCGANFGVMNLWD
                     GDSFNIAAGYNIPPAFAASRQHTSIRPHPESSLAALVRTHQVAHIDDLRNSPAYLAGA
                     PGAVEMADVAGARTIVFVPMLKENELIGTITIYRQEVRPFTDKQIALVENFTKQAVIA
                     IENTRLLKELRQRTDDLTESLEQQTATSEVLEIISSSAGELEPVFQKMLENATRICGA
                     NFGTMTLYDQGSYRTIAVYNAPAYAATQLNKLIHPHPMSGLGTIARTHQTVHIEDLRT
                     QPPYLEGNPTVVALSDLAGARTVVMVPMLKEDELIGVITIFRREVKPFTEKQTELVAN
                     FAKQAVIAIENTRLLKELRARTDDLSESLEQQTATSEVLEIISSSPGELEPVFQKMLE
                     NATRVCGANFGVMHLWDGDSFNIAADYNVPPAFAASREHMLIRPHPQSGLAAVVRTHQ
                     VVHIDDLRNGPAYLAGAPAVVEMADIAGARTIVIVPMLKENELIGTITIFRQEVRPFT
                     EKQIALVENFTKQAVIAIENTRLLKELRQRTDDLSESLQQQTATADVLKVISRSAFDL
                     KSVLTTLAESAKSLCGAAFGVIFLRDGDVLRDQAESGCPPALMNFLRDHPIRKSRATF
                     TGRVFMDGKVAHLPDVLLDPEYNFGAAPELGGYRAALAVPLMRDGGVEGVLSLARPDP
                     GPFTSRQIDLVQTFADQAVIAIENARLFGQVQERTRELSLSLDDLRTAQDRLVQTEKL
                     ASLGQLTAGIAHEIKNPLNFVNNFSALSAELIDELNDVLKPAALDGKIREEIDELTHM
                     LKGNLEKVVQHGKRADSIVKNMLLHSREGSGEHRPVDINTIVEESLNLAYHGARAERS
                     GFNITLRRDLDPEAGLIDLYPQEITRVFLNLISNGFYAATKRKEAGDDTFEPTLSATT
                     KSLGNQVEIRIRDNGTGIPLEVKEKMFNPFFTTKPAGEGTGLGLSMSHDIVVKQHGGK
                     IDVDTMPGVFTEFIITLPRTAAAQAQTGVSN"
BASE COUNT          719 a         1130 c         1112 g          785 t
ORIGIN      
        1 acatcgggct cgtcatcgac gacgaggatg taaacgctca atttgaaact ccagtttgcg
       61 cttgtgctgc cgccgtccgc ggcagcgtaa taatgaattc agtaaatacc ccaggcatcg
      121 tatcgacgtc gatcttgccg ccgtgttgtt tcaccacgat gtcgtggctc atcgacaggc
      181 caagtccggt gccctcgccc gcgggcttgg tggtgaagaa gggattgaac attttctcct
      241 tcacttccaa tgggatgccg gtgccattgt cgcgaatccg gatttccacc tggttgccga
      301 ggcttttggt ggttgcgctc agcgtcggtt caaatgtgtc gtcgccggct tccttgcgct
      361 tggtggcggc gtaaaagccg tttgaaatca gattgagaaa cacccgcgta atttcctgcg
      421 ggtagaggtc gatcaagccg gcttcgggat cgaggtcgcg ccgcagcgtg atgttaaagc
      481 cggagcgttc ggcgcgggcg ccgtgatagg ccagattgag gctctcctcg acaatcgtat
      541 tgatatcgac gggccggtgt tcgcccgacc cttcgcggga atgcagcagc atgttcttga
      601 cgatggagtc cgcacgcttg ccatgctgca ccaccttttc gagattgccc ttcagcatat
      661 gcgtcagctc gtcgatctcc tcccggatct tgccgtcgag cgcggccggc ttgagcacat
      721 cgttcagttc gtcgatcagt tccgccgaga gtgccgagaa attgttgacg aagttgagcg
      781 gattcttgat ttcatgggcg atgccggcgg tgagctgacc gagcgaggcc agcttctcgg
      841 tctgcaccag gcgatcctgc gcggtgcgca aatcatcgag cgagagcgac aattcacggg
      901 ttcgctcctg aacctgcccg aacaatcggg cgttctcgat ggcgatcacg gcctggtcgg
      961 cgaaggtctg caccaggtcg atctgtctgg atgtaaacgg gccagggtcg ggccttgcca
     1021 gcgagagcac gccttcaacg ccgccatcgc gcatcagcgg cacggcgaga gcggcgcgat
     1081 agccgccgag ttccggcgcc gccccgaaat tgtactctgg atccagcagt acgtccggta
     1141 ggtgcgcaac cttgccatcc atgaagaccc ggccggtaaa ggtcgcacgg ctcttgcgga
     1201 tcggatggtc gcgcagaaaa ttcatcagag cgggcggaca gcccgattcc gcctgatccc
     1261 gcagcacgtc gccgtcgcgc aggaagatca cgccgaaagc cgcaccgcac agcgacttcg
     1321 ccgactccgc cagtgtcgtg agcacggatt tcaggtcgaa tgccgagcgg ctgatgacct
     1381 tgagcacatc ggcggtcgcg gtctgctgtt gaagcgattc gctcaaatcg tcagtgcgct
     1441 gacgcagctc cttaagcagt cgtgtgttct caatggctat gacggcctgc ttcgtgaaat
     1501 tctcgaccag cgcgatctgc ttttcggtga acggccgaac ctcctggcgg aagatcgtaa
     1561 ttgtgccgat cagctcgttt tccttcaaca tcggcacgat gacgatcgtg cgagcacccg
     1621 cgatatcggc catttcgaca acggccgggg caccggcaag gtaagctgga ccgtttcgca
     1681 agtcatcaat gtgtacaacc tgatgggtcc tgacgacggc ggcgagaccg ctttgcggat
     1741 gaggacggat cagcatgtgc tcccgcgaag ccgcgaacgc aggcgggacg ttgtagtccg
     1801 cagcgatatt gaaactgtcc ccgtcccaca gatgcatcac gccgaaattc gctccgcaga
     1861 cgcgcgtggc gttctccagc attttctgaa aaaccggctc cagctcgcca ggcgaggaac
     1921 tgatgatttc aagaacctcc gaagtagccg tttgctgttc cagcgactcg ctcagatcgt
     1981 cggtgcgcgc gcgcagctcc ttgagcagtc gcgtattctc gatggcgatg acggcttgct
     2041 tggcgaagtt ggcgacaagc tccgtctgct tttcagtaaa tggcttgacc tccctgcgga
     2101 aaatcgtgat gacgccgatc aattcgtctt ctttgagcat cggcaccatg acaactgtcc
     2161 gggcgccagc aagatcggat agcgcgacaa cggttggatt gccctcgaga tacggcggct
     2221 gcgttcgaag atcctcgatg tgaaccgtct gatgggtcct cgcaattgtc ccaaggccgc
     2281 tcatcggatg cggatggatc agcttattca actgggttgc tgcgtaggcc ggcgcattgt
     2341 acaccgcaat cgtccggtag ctgccttggt cgtacagggt catcgtacca aaatttgctc
     2401 cgcagatacg cgttgcgttt tcgagcatct tctggaaaac cggctccagt tcgccggccg
     2461 aagaactgat gatttcgaga acctccgagg tcgccgtctg ctgctccagt gattcggtca
     2521 aatcatcggt gcgttgacgc aattccttca gcaatcgcgt attctcgatg gcgatgacgg
     2581 cctgcttggt gaaattctcg accagcgcga tctgcttgtc ggtgaaaggc cggacctcct
     2641 ggcgatagat ggtaattgtg ccgatcagct cgttttcctt caacatcggc acgaagacaa
     2701 tggtgcgagc accagcgaca tcggccatct caacagcgcc gggggcacca gcaaggtaag
     2761 ccggactgtt tcgtaggtca tcaatgtgtg cgacctgatg agtcctgacg agggcggcga
     2821 gactgctttc cggatgggga cggatcgacg tgtgttgccg tgaagccgcg aacgcaggcg
     2881 ggatgttgta gcccgcagcg atattgaagc tgtccccatc ccacagattc atcacgccga
     2941 aattcgctcc gcagacgcgc gtggcattct ccagcatttt ctggaacaca ggctccagtt
     3001 cgcccggcga cgaactgatg acttccagca cttccgaggt cgcggtctgc tgctccagtg
     3061 actcggtcaa ttccgccgtc cgcgtctcga ccttcttctc caaatctgca taggactcct
     3121 gcagccgcgc gcccatgtcg ttgaactgat tggcgaggcc ttccaactcg tcgccggtcc
     3181 gaatcgagat gcgctgggcg aaatcgccgc cgccgatacg ctcggcgccg gcgcgcaagg
     3241 cctggatcgg gccgaccatg cgccgagcca aaaatatccc ggcgagcacc gcgaagatca
     3301 acgcggccgc cagcaccgtg gccagcctct gcagcgccgc gtacagcgac gcataggctt
     3361 ccttgaccgg aagttccacg aacatggtcc agccaagcgg cgcgatcggc gcggatgccg
     3421 tcagcacctc ctgcccctgg atattcagcg cgccctgcag cgcctcggcg tccgtgctgc
     3481 cgctttgtgc cgcctgcacc tgcaccagct tcgacatatc ggtgttacgc agcacaagag
     3541 agatgtcggg atgcgcgatc aggcgtcctt ccgcgccgac aacataggcg tgaccgcgct
     3601 cgccgacctt gatctgggag accacgtccc agatcagctt cagattgacc tcggcaatgc
     3661 tgacgccggc atccttgcgg gtgccggcca gcgcgatcgt catgtagggc tcggactccc
     3721 ggcggaaata gaccggcccg taatag
//
LOCUS       DASWGM010000349        11172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_441554,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000349 DASWGM010000000
VERSION     DASWGM010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441554"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..2897
                     /locus_tag="VGA15_10090"
                     /pseudo
     CDS             136..2897
                     /locus_tag="VGA15_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            3128..4324
                     /locus_tag="VGA15_10095"
     CDS             3128..4324
                     /locus_tag="VGA15_10095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9588082.1"
                     /translation="MKDSIGGRPQAGAASPDRDRLAPLGDIAASQWRRLAERAVEPNG
                     YYLPDWELAVNAFARGRTGVSALGAWADAPTAPNGTTSLIGLVPVISLWRACRIPLPA
                     LVSADPYGTLSTPLIDRDMANDAVAGLMLEARKAGAHALILRDVPLDGAAIKAFTELL
                     QKDGMRPLVLQSHVRACLDATRDADELLRNALGPKKLKELRRQRHRLAEHGAVRFDVA
                     RTPTDVASAVEIFLHLEASGWKAKRGTALMQDEGDASFVRRAAKALAEIGQCEIVTLR
                     AGDTAVAAAIVLRHLDRAFYFKLGVDERFAKLSPGVQLTLDLTRHLCADPAMAMADST
                     AGADHPMINPIWRGRLAIGDVLIPLRRNDLMVSLIHAAMALRRLIREPVRRAVHFVRR
                     RQETSS"
     gene            4321..5266
                     /locus_tag="VGA15_10100"
                     /pseudo
     CDS             4321..5266
                     /locus_tag="VGA15_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(5301..5507)
                     /locus_tag="VGA15_10105"
     CDS             complement(5301..5507)
                     /locus_tag="VGA15_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9588083.1"
                     /translation="MKSVSGRDFARIIERRGWTLLRISGSHHIYGKSDSVVRLSIPIH
                     GNKPLKTGLLRHLAKLADLSDEDL"
     gene            complement(5504..5707)
                     /locus_tag="VGA15_10110"
     CDS             complement(5504..5707)
                     /locus_tag="VGA15_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9588084.1"
                     /translation="MKIKIVVHEAEEGGFWAEVPAIPGCASQGDSMDELMKNLQEAIE
                     GCLSIEVAPREPGGTERMLELPA"
     gene            complement(5774..6439)
                     /locus_tag="VGA15_10115"
     CDS             complement(5774..6439)
                     /locus_tag="VGA15_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX9588085.1"
                     /translation="MSSLKPLQIDIVSDVVCPWCYIGKRRIENALALAPDVPVEINWR
                     PFFLNSWVPREGISRDEYLTAKFGSPEAYKGIAGRVVAAASEEGLTYRPDLVKRQPNT
                     IDCHRLIHWADAIGKSAEMKQRLMELYFRDGGDLTDRNVLVQAAADCGLDADDIRERL
                     AADEDVDLISAQAKDASDKGISGVPTFVFAQKYAVSGAQPAEQLARAIRQVSGEINAQ
                     AAE"
     gene            6600..7382
                     /locus_tag="VGA15_10120"
     CDS             6600..7382
                     /locus_tag="VGA15_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9588086.1"
                     /translation="MTAIFMPEGHLFRATDEAGGPWSADMLQGSATTALMVREVERLA
                     ASSGFAVRRLTFDLWRPAGLRAFRAQTEMLRDGRKAKTIQVRPMDGEVEIGRCTALLT
                     APGESPADPFATPAAIDPPPESGTPPPAFAQKWSRYFQNVSVRLIEGALEKPGPAAAW
                     MRLDVPLVEGEANTALLQAVQAADFSSGVAQIVDMRVWTFVNPEISLYFFRAPEGEWI
                     LIRSRTRVGAHGAGLTTASLSDRQGPFAEVMQAMTFEKRAAA"
     gene            complement(7389..7523)
                     /locus_tag="VGA15_10125"
     CDS             complement(7389..7523)
                     /locus_tag="VGA15_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588087.1"
                     /translation="MGLIGAVLTLGTPQDVTLQELRVEMFMPADAESEAVLVALANLD
                     "
     gene            complement(7514..8212)
                     /locus_tag="VGA15_10130"
     CDS             complement(7514..8212)
                     /locus_tag="VGA15_10130"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9588088.1"
                     /translation="MDSRSARSASLRPHPVGDLLRDWRTRRAMSQAGLAFEAGISTKH
                     LSCVETGKAAGSREVLLRLAAALGLSLRDRNALLEAGGFARQYGERDLSAPEVADAKR
                     AIDLLLRRHEPFPAIVTDRRWNVMQANRAATGLMTLLLGTERMSRPLNHMKMFLAPDE
                     LRPFVENWPEVAAALMVRARHEAMSAPLDLALQSTWRELTGLPDVAPPQFNEGEAPGP
                     LCRYVCAKAMSAWA"
     gene            complement(8359..8529)
                     /locus_tag="VGA15_10135"
     CDS             complement(8359..8529)
                     /locus_tag="VGA15_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX9588089.1"
                     /translation="MKARHPEIPWRKVSGSGNIYRHEYEDVAHRLVWGTVHEFLPALR
                     VMAKTELAAEGC"
     gene            complement(8594..8707)
                     /locus_tag="VGA15_10140"
                     /pseudo
     CDS             complement(8594..8707)
                     /locus_tag="VGA15_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8697..8993)
                     /locus_tag="VGA15_10145"
     CDS             complement(8697..8993)
                     /locus_tag="VGA15_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9588090.1"
                     /translation="MTRDEIIEKLKEREADLRAQGVAHAALFGSIARGEDRPDSDIDI
                     LVDLDPAIVVTIFDYAGVKDFIADMFDRPVDVVSRESLKPRLRPKATADAIYAF"
     gene            complement(9183..9998)
                     /locus_tag="VGA15_10150"
     CDS             complement(9183..9998)
                     /locus_tag="VGA15_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9588091.1"
                     /translation="MNAHTATARADRVKPAHIGDHLREWRQRRHLSQLDLAGDAEISA
                     RHLSFVETGRAAPSRDMVLKLAERLDVPLRERNVLLVAAGFAPAFPQRSLDDPALKSA
                     RAAIDLVLRAHEPNPALAYDRHWNLVSANRMVMPLLEGIPARLLGQPFNILRLAFHPE
                     ALAARTVNLAEWCGHLLERLHRQCEATADPDLIKLYSELKAYPIPARSAPLSADSVAL
                     PFKMRLDGETLSFFSTTMIFGTPVDITLSELAVETFFPADDLTAERMRAMQPR"
     gene            10099..10350
                     /locus_tag="VGA15_10155"
     CDS             10099..10350
                     /locus_tag="VGA15_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588092.1"
                     /translation="MRTPSRSSTVSPAASMIPWLLNLATRMLACALNMPEPLLQNTGV
                     FVFAHVQAVENSIGKTLCPIRLLRQFRSLLTSLRRQPAR"
     gene            10390..10788
                     /locus_tag="VGA15_10160"
     CDS             10390..10788
                     /locus_tag="VGA15_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588093.1"
                     /translation="MIHPSQFLCRAILGDAIFSGASAVLLTFGAGELAPLFDLPEALL
                     LETGLFLIAYAVGVGWLGTRQAMPKALVVIVIAGNAAWTLASIALLFSGAVTPNLLGE
                     IFIAAQAVAVGALAELQYIGLRRSGGALAA"
     gene            10980..>11172
                     /locus_tag="VGA15_10165"
     CDS             10980..>11172
                     /locus_tag="VGA15_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9588094.1"
                     /translation="MTSVQVSGTEGYAEEAEDLFKRYESIPAADAHRAVLHLIPAAPS
                     RILDIGSGTGRDAAWLASMG"
BASE COUNT         2027 a         3554 c         3476 g         2115 t
ORIGIN      
        1 aatggaaatg gcaacggcaa tggccacggc ctcgcgaagg ggcacaacaa gtaaggccga
       61 accatttttt cgaaccgctg caccgagcat ggcagtgcgc ctggccggtc gcgaaactta
      121 ggggattggg acaacttgaa aaattatcga ccgcatatcc ttgtggtggt tgcgctggcc
      181 atcgtgctgt cgtccggctg gcacggcatg ctccgcaatg ccctgaccga tttgcgcttc
      241 gcctgggtgc cgcgtcaggc caccggcaac gttgtcgttg tcgcgatcga tgctccatcg
      301 atcgagaaaa tcggcgtctg gccatggcca cgccgcctcc atgccgactt gctgcggcgg
      361 ctggaaagcg ctgatgttcg ggacatcgta ttcgacgtcg atttcagcac gccgtccgac
      421 gctgcggcag accaggcatt tgccgatgcg ctgcgaagcg cgggcggatc cgtggtgttg
      481 ccgtccttca agcagccggg agcctacggc ggcgatgcaa cggctgttca tatcaatcgt
      541 ccgctgaagc tatttggcga tcattcatgg ccggcggtcg tgaatgtcgc cattgagccg
      601 gatggccttg tccggcacta cccctttggc gagaacctcg acggacaatt tctgccatcg
      661 atgggcgcgg tattggcggg gcaatatgcg gtaaagcgcg agccattcct gatcgatttc
      721 agtatcctga ccgcgtccat cccgaaggtg tcctacatcg acgtgctgcg tggcgatgag
      781 gcgacgctgg ccaagctccg ggacaagaag gtcgtcatcg gcggcaccgc ccttgaactc
      841 ggcgatcgct tcagcgttcc gaatggcaac ctcgtctccg gacctctgct gcaggccctc
      901 gcggccgaat cgatcctgca gaaccgcatc ctgcgccaga cctcggatat cgtgacgctg
      961 gctggactat gcgtcatcgc gctgatcgtg atggtctcgt ggcgccgcct ccccgccggc
     1021 accagggttg tcgtgcttgc cggaatggct gccgcggttg aagccggcgc aaccgtgctg
     1081 caggccaagc tgcccctcgt tctcgatacg tcgctgtttc ataccgcgat cgccgtctac
     1141 acggccgcca ttgcactgga tgaaattgat ttccggggtt tgctgggccg catcgccgag
     1201 aaccgctttc agcgcatcgc gatgtcgctg ggcgacggcc tggtgtgcac cgactcgaac
     1261 cacctgatca cggtctggaa tcccggagcg ttgcgatttt cggctacgac gctgcggaaa
     1321 tgatcggcca gccgttcgag cggatttgcg ccgcccacac caaggccccg cccgaagcaa
     1381 tttcggcctg cgaggaagcg cgcgcccgat cgcagcgcgc gggcggcata gtcatggaat
     1441 tcgacagccg ccacaagaac ggcgatgtgt ttccggtcga ggcctgcttg tccggttggc
     1501 tgggcaccga tggctttcaa tatggcgtga tcctgcgcga catatcggtg cgaaagcgcg
     1561 aagccgaacg gatcagatat ctggcggaat acgatacgct gaccggactg gccaatcgca
     1621 acacccttca ctcggaactg gctgcgatgc tagccagcgc cgaaaacgcg gcgggcgaag
     1681 tagcgctgct ggtggttggc ctcgacggct tccagcaaat caacgacatg ctgggccatg
     1741 cctgcggcga cctcgtgtta cgtgccgtgt ccgaacgatt gaaggccgag atggagggcg
     1801 caggagtagt cgcacggctg agcggcgatg aatttgccat cgcgatcccg tccgtcgaaa
     1861 tgaccgaaac cgtcgcacaa ttatccgaac gcatctcgct cacgttcaag acgccgcttt
     1921 tgactggaac gcgtctgcac cgggtcaagg ttaatatcgg cgtagccatc tatccaggcg
     1981 gcggcatgac ggccgacgaa ctgctgggca acagccatct ggcgttttgt cgcgccaagg
     2041 caaccaagcg cggcggtcac gtgctattcg atggcagcat cagagcggaa cttgaggcac
     2101 ggctgacgct ggaggccgaa ttggttcgcg ccgccgagcg caaggaattc gaattgttct
     2161 atcagccaca ggtccgtctg gcggacggcg gcctgatcgg cgcggaagcc cttattcgct
     2221 ggcgccatcc cgttcgtggc ctggtctcac ccgcggaatt catgccggtc gtcaatactt
     2281 catcggcctc ggatcggatt gcgggctggg ttctcgaaac cgcgtgcaga caggcgcggg
     2341 tttgggagca agccggacat gacgtccgcg tcggcatcaa cctttcgccc tcgcaacttc
     2401 ggtcgggcga ccttgcaaag tcggtcgcgg aggtccttga ggtcaccggc ctgacgcctt
     2461 ctctgctcga actggaggtg accgaggaca tcctgctgtt ggacgaggag agagtgctcg
     2521 acatatttcg acggattcaa gaactcggcg ttcgcgttgt tttcgacgac ttcggcaccg
     2581 gttatgccag cctcagctat ctcaagaaat ttccgctcga tggactcaag atcgacaggt
     2641 cattcgtgat cgagctgcgc gccgattccg acgatgcggc gatcgtcgga tcgaccgtcg
     2701 gtctttccag gcaactgggc ttgtcggtga tcgcggaagg catcgagacc cgggccaccg
     2761 ccgatttgct gatgagcatg ggctgcgaag agggacaggg ttttttcttc ggccggccga
     2821 tgccggccgc cgcattcgaa agccaattcc tggcgacgca cgacgccgaa gcgctcgaac
     2881 gcggccacgc agcctagcaa cggttttgct cgccgctacg gcggtccggc gcccgaatga
     2941 taaacgcccg gttaacaata tcccttgaat tgaatctccg caccaacggg attttccaat
     3001 ctttccgcta gcatttccct gacgacgcgg tggcaggagc ggcgctattt ggctccgcgg
     3061 gttccgcaaa cggggaaccg gggacgtggt cgagattgcg aattcagcgg tggtccgccg
     3121 cgcattgctg aaggatagca tcggtgggcg cccgcaggcg ggagcagctt cgcccgatcg
     3181 cgataggctc gcgccgctcg gcgacatcgc cgcgagccaa tggcgccggc ttgccgaacg
     3241 cgccgtcgag ccgaacggct attatctgcc cgactgggaa ttggcggtca acgccttcgc
     3301 acgcggccgc accggcgtat ccgcgctcgg cgcctgggcc gatgcaccga cggcgccaaa
     3361 cggaacgacg agcctgatcg gcctggtgcc ggtgatttcg ctgtggcgcg cctgcaggat
     3421 cccgctgccc gctttggtga gcgccgatcc ctacggcacc ctttccacgc cgctgatcga
     3481 tcgcgacatg gcgaacgacg ccgtcgccgg gctgatgctt gaggcgcgaa aagcaggcgc
     3541 ccatgcgctg atcctgcgcg acgtgccgct ggatggcgcg gccatcaagg ctttcacgga
     3601 actattgcag aaagacggca tgcgtccgct ggtgctgcaa tcccatgtcc gcgcctgtct
     3661 cgatgcgacg cgggatgccg atgaactgct gcgcaatgcg ctgggaccca agaaactcaa
     3721 ggagctgcgc cgccagcgcc atcgcctcgc cgaacacggc gccgtccgtt tcgatgtggc
     3781 gcgtacgccc acagacgtcg catccgcggt cgagatattc ctccacctcg aagccagcgg
     3841 ctggaaggcc aagcgcggta ccgcgctcat gcaggacgag ggcgatgcca gctttgtccg
     3901 gcgtgcggcc aaagcgctcg ctgaaatcgg ccaatgcgag atcgtaacgc tgcgggccgg
     3961 cgatacggcg gtcgcggctg cgattgtgct ccggcacctg gatcgcgcat tctacttcaa
     4021 gctcggcgtc gacgagcggt ttgccaaact gtcgcccggc gtgcagctga cgctggatct
     4081 gacgcgacac ctgtgcgccg atcccgcgat ggcgatggcc gattccaccg cgggcgccga
     4141 tcatccgatg atcaatccga tctggcgcgg ccgccttgcg atcggcgacg tcctgattcc
     4201 gctgcggcga aacgatctca tggtgtcgct gatccatgcg gcgatggcgc tgcgccggtt
     4261 gatccgcgaa ccggtgcgcc gcgccgttca tttcgtgcgc agacgccagg agacatcgtc
     4321 atgaacgagg tcaccgccat tgcaccggtg attaccgccg gcaacgccgc gctgaggcgc
     4381 gactttcccc taaaaccatt cgcgatccgc cacaagctgg caggccatcc gctgctgacg
     4441 ctgccgcgca tcgcgcagct tgccgccgag ctgccgcgcg acctgatcga atacaattca
     4501 ggcaaggtcg cgatcagcca ggatccggat gcgatccctc acctcgatct cgatccggtc
     4561 gaagtggtga agagcatcga gacctcgggc gcatggacgg tgctcaagcg cgtggagaat
     4621 tcgccgcaat atcgccggct gctggaagac acgctgcttt tcggtggccc gcgcgcgcgg
     4681 cttcaacagc ctgctcgacg ccgacttcga gcaggtcgag ggtttcttgt ccgtgtcctc
     4741 gccgaattcg accacgccgt tccatctcga cagcgaagac tgtttcttcg tgcacatcca
     4801 cggcgaaaag ttcttcacga tcgtcgacaa cagcgaccgc gccatcgtcc ccgacgacga
     4861 aatcgagcgc tcgatgacca ggcatcgcta cctgaaatac gacgagcgtt ttgcgtcgcg
     4921 cggcgtcgaa tttcacctgt tcgcgggcga tggctgcttt gtgccgtatc agtggccgca
     4981 ctgggtgcgg accgcggatt catattcgat ctcgatggcg atcacctgga agacccgcga
     5041 agtgcgccgc cgcaacgatt tgcatttctt caattcaatg ctgcgcggac tgggcctgcc
     5101 gcaacagccg ccgggcgtcc agccggtgcg ggatgcgtta aagctcacct tctatcgcac
     5161 cgtgaccacg gcgatccgcc ccttgcgcgc ctcgctggcg atgcgccgtg tgctccgcag
     5221 gatagcgctc ggcaaacgcg cgaattatta tttgaagggg gcgtgaggct cgccgaccct
     5281 cggcgttggt gcgcccgccg ctaaagatct tcatccgaaa gatctgcgag tttcgccaaa
     5341 tgccttagta ggcccgtctt cagtggcttg ttgccatgga ttggaattga aagccgcacg
     5401 acgctgtcgc ttttgccgta gatatgatgg ctgccgctaa tgcgaagcaa ggtccaaccg
     5461 cgccgctcaa taatccgggc gaagtcgcga ccggataccg atttcatgca ggcaattcca
     5521 gcatccgctc ggtgccgccc ggctcgcgag gggcaacttc aatggaaaga catccttcaa
     5581 tcgcttcctg gagatttttc atgagttcat ccatgctgtc gccttgacta gcacagcccg
     5641 gaatggcggg gacttcggcc caaaagcctc cctcctcagc ctcatgcacc acgatcttaa
     5701 tcttcatcgt caatctccaa atccgattgt cgcgcggaca cccggccaat tcaagtcttg
     5761 cggaagcttt atgtcactcc gccgcctgtg cgttgatctc tcccgacacc tgacggatgg
     5821 cgcgggcgag ttgctcggcc ggctgggcgc cggagaccgc gtatttctgc gcaaacacaa
     5881 atgtcggcac gccggagatc cccttgtcgg aggcgtcctt ggcctgcgcc gagatcaaat
     5941 cgacgtcctc atcggcggcg agacgctcgc ggatatcgtc ggcgtcgagc ccgcaatcgg
     6001 ccgccgcctg caccagcacg ttgcggtcgg tgagatcgcc gccgtccctg aaatacaatt
     6061 ccatcagccg ctgcttcatc tcggccgatt tgcctatcgc atcggcccag tgaatcaggc
     6121 gatggcagtc gatcgtgttg ggctggcgct tgacgagatc gggccggtac gtaaggccct
     6181 cttcgctcgc ggcggcaacg acgcgcccgg cgatgccctt gtaggcctcg ggcgagccga
     6241 atttcgcggt gaggtattcg tcgcggctga tgccctcgcg cggcacccag gaattgagaa
     6301 agaacggccg ccagttgatc tcgaccggca cgtccggcgc cagcgccagc gcgttttcga
     6361 tccggcgctt gccgatgtag caccacgggc acaccacgtc ggagacgata tcgatctgaa
     6421 gcggttttag gctgctcatc gggagtgtcc tcgctgcaac ggtggctcaa aaataggccg
     6481 aaccggctgc gaaacaagtg cgcaggaagc atgcccagcc ccacataacc tccggggtta
     6541 ttgtggatgc gggcgggcgt tcgtagaagg tagccaacga taggccgcgg agcccagcga
     6601 tgaccgccat cttcatgccg gaaggccatc tgtttcgcgc caccgacgag gccggcggcc
     6661 cctggtcggc ggacatgcta cagggcagcg ccaccaccgc gctgatggtg cgcgaggtcg
     6721 agcggctggc ggcctcgtct ggtttcgcgg tgcgccgcct caccttcgat ctatggcggc
     6781 cggccggatt gcgtgccttc cgcgctcaga ccgagatgct gcgcgacggc cgcaaggcca
     6841 aaacgatcca ggtccggccg atggacggcg aagtcgagat cggccgctgt acggcattat
     6901 tgaccgcgcc gggcgagtcg ccggcagatc ccttcgcgac accagcggcc atcgatcctc
     6961 cgcccgagag cggcacgccg ccaccggcct tcgcgcagaa atggagccgt tacttccaga
     7021 acgtatcggt tcggctgatc gagggtgcgc tggaaaagcc ggggccggcg gcggcctgga
     7081 tgcgcctcga cgtgcccctg gtcgagggcg aagccaacac ggcgctattg caggcggtgc
     7141 aggcggcgga cttctccagc ggcgtcgcgc agatcgtcga catgcgcgta tggacttttg
     7201 tcaatccgga gatcagcctg tatttctttc gtgcccccga gggcgagtgg attctgatcc
     7261 gctcgcgcac ccgcgtcggc gcccacggcg cggggttgac gaccgcgagt ttgagcgatc
     7321 ggcaaggtcc gttcgcggaa gtcatgcagg cgatgacgtt cgagaagcgg gccgcagctt
     7381 aagcttgctc aatcaagatt tgcgagcgcg acaagcacgg cctcggactc cgcgtcggcc
     7441 ggcatgaaca tctccacgcg caattcctgc agcgtcacat cctgcggcgt acccagcgtc
     7501 agcaccgcgc cgatcaggcc catgctgaca tcgcctttgc gcaaacgtac ctgcacagcg
     7561 gccccggcgc ttcgccctcg ttgaattgcg gcggcgccac atcaggcagt cctgtcagtt
     7621 cgcgccaggt cgattgcagc gcgagatcga gcggtgctga catcgcctca tggcgcgcac
     7681 gaaccatgag cgccgcggcg acctccggcc agttctccac gaacgggcgc aattcgtccg
     7741 gggcgagaaa catcttcatg tgattgagcg gacgactcat gcgctcggtg ccgagcagca
     7801 gggtcatcag tcccgttgcc gcgcggttgg cttgcatcac attccatcgc cggtcggtga
     7861 cgatagccgg aaacggctcg tgacgccgca gcagcagatc gatggcgcgt ttggcgtcgg
     7921 caacctccgg cgcggacaga tcgcgctcgc catattgccg ggcgaagccg ccggcttcca
     7981 atagcgcgtt gcggtcgcgc agcgacagcc cgagcgctgc ggcaagcctg agcagaacct
     8041 cgcggctgcc agccgccttc ccggtctcga cacagctcag atgttttgtc gagatgccgg
     8101 cctcgaaggc gagaccggcc tggctcatgg cccgccgggt gcgccagtcg cgcaggagat
     8161 cgccgaccgg atgaggccgc aagctggcac ttcgcgcgga gcgtgaatcc atcgggcgat
     8221 tatgcgaact gcgcacccgg ctgtaaacgg ccgtctcagg gtcgctgccg gcgtgccacc
     8281 ccagcacgca gcgcaactgt tgccgatcgc gcgtgtgctg gttaccgata cgcgagctta
     8341 gccgtagatg gccatggcct aacagccttc ggctgcgagc tcggttttgg ccataacccg
     8401 cagcgccggc aaaaactcat gcacggtgcc ccatacaagg cgatgggcca cgtcctcgta
     8461 ctcgtgccgg tagatgttgc cggagccgga caccttgcgc caagggatct cgggatggcg
     8521 cgccttcatt tccgaaagac aggcggcgcg acgcttccga gatgatctcg agacatcgcg
     8581 tgatggcgta aaacgtccgc atatccgcca ggaagtgctc gtagtcgaac ccctgggcaa
     8641 aggaatttgc gagatcgatg ttgaaaagaa tgtccgacag gctgtcgcgt tcggtttcag
     8701 aaagcataga tcgcgtcggc cgtagccttg ggccggagac gcggtttcag actttcgcgg
     8761 ctgaccacat cgaccgggcg gtcgaacatg tcggcgatga aatccttcac gccggcataa
     8821 tcgaaaatcg tcacgacaat tgcgggatcg agatcaacca ggatgtcgat atcgctgtca
     8881 gggcggtcct cgccgcgcgc gatggaaccg aacagcgcag catgggcgac gccttgcgcc
     8941 cgcaagtcgg cctcgcgctc tttcagtttt tctatgatct cgtcgcgtgt catggacaac
     9001 attgtacggt aaagagagta aaaaaacacg ttctcttttg cctcctttgc acgtcattta
     9061 tatatagaaa acgcctttgt ccgccagtgg cccagctgtt gtgcatattg cctgaaggcc
     9121 gccccgagcg tgaatccatg ttgcgattat gcgaactggg ttcacttctg taaacggccg
     9181 tctcagcgcg gttgcatcgc ccgcatccgc tcggcggtca gatcatcggc cggaaaaaac
     9241 gtctcgacgg cgagttccga cagcgtgata tcgaccggcg tgccgaaaat catcgtggtg
     9301 gagaaaaagc tcaaggtctc gccgtcgagc cgcatcttga agggtaatgc gacgctgtcg
     9361 gccgacagcg gcgcggaccg cgccggaatc ggataggcct tcagctcaga atagagtttt
     9421 atcaagtcgg gatccgccgt cgcctcgcac tggcgatgca gccgctccag gagatgcccg
     9481 caccattcgg cgagattgac ggtgcgcgcc gccagcgcct ccgggtgaaa ggcgagccgc
     9541 agaatgttga agggctggcc gagcagtcgc gccggaatcc cttccagcag cggcatcacc
     9601 atgcgatttg ccgagaccag attccagtgc cggtcatacg ccagcgccgg attgggctca
     9661 tgcgctcgca gcaccagatc gatcgcagcc cgcgccgatt tcagcgcggg atcgtcgagc
     9721 gatcgctgcg gaaacgccgg cgcaaatccc gccgccacca gcagcacatt gcgctcacgc
     9781 aacggtacgt cgagccgctc cgccagcttc agcaccatgt cgcgcgacgg cgccgcgcgg
     9841 ccggtttcca cgaagcttaa atggcgcgcg gaaatctcgg cgtcgccggc aagatcgagc
     9901 tggctgagat ggcggcgttg ccgccattcg cgcaagtgat cgccgatatg ggcgggctta
     9961 actcggtcgg ctcgcgccgt ggctgtgtgt gcgttcatgc tgcaaaacct atcacgcgaa
    10021 tttcatgcgt tccattacct ccgacgtaat cgaactccct atccgccccc gtcatcttcg
    10081 ccttcacagg agatgaccat gcgcacgcct tcccgatcca gcaccgtatc cccagccgcc
    10141 agcatgatcc cgtggcttct caatctggca acccggatgc tggcgtgtgc gctcaacatg
    10201 cccgagccgc tgctccaaaa caccggcgtc ttcgtgttcg cgcatgtgca agcggtcgag
    10261 aacagcatcg gcaagacgct gtgcccgatc cgcctgctgc gtcagttccg gtctctgctc
    10321 acgtccttgc gccgccagcc ggcgcgttga ccctcaccgt ccactcaacc ccgaaaaagg
    10381 agaactgcca tgatccatcc atcccagttt ctgtgccgtg ccatcttggg cgacgccatc
    10441 ttcagcggcg cgtcggccgt gctgctcacc ttcggtgccg gcgagctcgc gcccttgttc
    10501 gacctgcccg aagcgctgct gcttgagacc ggcttgtttc tgatcgccta cgccgtgggg
    10561 gtcggctggc tcggcacccg gcaagccatg ccgaaggcgt tggtggtcat tgtgatcgcc
    10621 ggcaacgcgg cgtggaccct tgccagcatc gcgctgttgt tctctggcgc ggtaacaccg
    10681 aatctgcttg gcgaaatctt tatcgcagcg caggcggttg cggtcggcgc cttggccgag
    10741 ttgcagtata tcggcctgcg gcgaagcggc ggcgcgctcg cggcctaaaa tcgtcgttgc
    10801 gaacgcaagc gaagcatccg aagctgtcat tccggggcga tgcgaagcat cgaacccgga
    10861 atctcgagat tccgggtccg gtgctacgca ccatcccgga atgacggagg atgggattgc
    10921 ttcgtcgttg cgctcctcgc aatgacggga agcacggatc ggacgttcgg agatatcgcg
    10981 tgacgtcagt tcaagtgagc ggcaccgaag gctatgccga ggaggccgaa gaccttttca
    11041 agcgctatga aagtatcccc gccgccgacg cgcaccgggc ggtgctgcat ctgatcccgg
    11101 cggcgcccag ccgcattctc gacatcggtt ccggaaccgg gcgcgatgcg gcgtggctcg
    11161 cgtcgatggg cc
//
LOCUS       DASWGM010000350         6318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2393522,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000350 DASWGM010000000
VERSION     DASWGM010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2393522"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VGA15_10170"
     CDS             complement(<1..628)
                     /locus_tag="VGA15_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX9588095.1"
                     /translation="MSATIGALGVVYGDIGTSPLYALKEAARAAAHGGPIAPEAILGV
                     VSLILWALILIISLKYALLILRADNRGEGGIVALLALLSARNARPGTWRAQLLVVGLV
                     GAALLYGDGAITPAISVLSAIEGLKVDAPSLAPAVVPLTIAILIGLFIVQKKGAGFIG
                     NIFGPVMLAWFVVIALLGVHGIVRAPGVLAALSPLYAFDLLIHEDFHIS"
     gene            complement(826..1086)
                     /locus_tag="VGA15_10175"
     CDS             complement(826..1086)
                     /locus_tag="VGA15_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588096.1"
                     /translation="MIKALSAIAIAAFVAAALTVLPGFAPQVEASVPVPLAKADRLDI
                     HTVGPNCSQKAWPNFEASCLRAAGKKTVTIKEARLVTADRAP"
     gene            1369..1866
                     /gene="coaD"
                     /locus_tag="VGA15_10180"
     CDS             1369..1866
                     /gene="coaD"
                     /locus_tag="VGA15_10180"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX9588097.1"
                     /translation="MPRIALYPGSFDPVTDGHLDVVRHAVGLCDRLVVAIGVNSSKKP
                     MFSIDERLEMVEEVFAPVAAAAGCAFDCTTYDNLTVTAAQKHGATIMIRGLRDGTDLD
                     FEMQIAGMNETMAPDVHTVFVPASAAVRPITATLVRQIATMGGDVSAFVPPSVAARLK
                     AKFKG"
     gene            1918..2460
                     /locus_tag="VGA15_10185"
     CDS             1918..2460
                     /locus_tag="VGA15_10185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9588098.1"
                     /translation="MIRILAIVGALMCAAPAIAQPLPANLDKPNAIVIDTTKGRIVLK
                     LRTDLAPKHAERIKQLARDGYYNNVPFHRVIEGFMAQTGDGKNFNGTGGSKYPNLPAE
                     FSSVPFKRGIVGMARASDPNSANSQFFIMFADGSSLNGQYTVIGEVVSGMDVVDKLKR
                     GEPVTDPDKMVKVQVASDLK"
     gene            2509..2907
                     /locus_tag="VGA15_10190"
     CDS             2509..2907
                     /locus_tag="VGA15_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588099.1"
                     /translation="MLFASSSLMAQAQPAQVNTLKEVFAKLGSCWKPPPASQANPGID
                     ITVIVSFNRAGEILGHPKITYESEQATDNDRLAYRVAVMEALQRCTPMPFTETMAGAV
                     AGRPFAVQFRNRKSPPKPIEKRAWLTPKTL"
     gene            2883..3350
                     /locus_tag="VGA15_10195"
     CDS             2883..3350
                     /locus_tag="VGA15_10195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX9588100.1"
                     /translation="MADTENTLILETTQGPVTIEMRPDLAPGHVARIKELVREGFYDG
                     IVFHRVIEGFMAQTGCPQGTGTGGSGKKLEAEFNKEPHVRGTTSMARASSPDSGDSQF
                     FICFDDASFLNNQYTVWGKVTEGMENVDKIKRGEPVQNPDKIVKARMALDDAA"
     gene            complement(3697..4185)
                     /locus_tag="VGA15_10200"
     CDS             complement(3697..4185)
                     /locus_tag="VGA15_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9588101.1"
                     /translation="MKRIFAICAAVAAIGLMASSARAEISTEAARDSVAPFYKALNAE
                     FAKDSPELIKQSTAPQWISCRGNDVCNSRDEVIAGIGQRLKSVPDLKWEIKEILVSGN
                     QVTVRGEATGTPAGEFMGTPHTGKSFKLMSIDVHTLEGGKIVRSYHIEDWLGAVRQLS
                     AK"
     gene            4269..5195
                     /locus_tag="VGA15_10205"
     CDS             4269..5195
                     /locus_tag="VGA15_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9588102.1"
                     /translation="MNEIDLRRFDLNLLVVFEVLMAERNVTRAAERLGRTQSAVSHSL
                     SRLRSQLGDPLLIKGGVRMQPTAFAIDLMEQARPMLRGIERVLSPRHVFDPACSQRVF
                     RLAAPDFASTLFTSLLARLRSDAPGVSVEWTTAREPTLLDVAEGQVDVAIVPAQLRMP
                     DGVNSEAIGALKWRCFGRQGHPAFSKWGRKSWSHWPHLAVRVGDSLTSPVNLAAGAAR
                     LERRIAGWVPHFSAIAPILAGSDLLATLPSIAMAATLHPYRLDSREVPFPIAPLPHAM
                     IWSTGRSSHPEVRWLRDRLRPVVKSKFTDLAG"
     gene            5319..>6318
                     /gene="queA"
                     /locus_tag="VGA15_10210"
     CDS             5319..>6318
                     /gene="queA"
                     /locus_tag="VGA15_10210"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX9588103.1"
                     /translation="MRTDLFDFELPTGHIALRPASPRDGARMLIVQPDGVLRDDIVAD
                     LPHWLEPGDQLVVNDTRVISAQLSGRRIGRETEPKIEATLIKRLDGSRWQALVRPAKK
                     LSRGDVVRFGHEGRVCLLGHLDAQVEHKGDDGEVTLSFSFHGPALDSAIADLGRPPLP
                     PYIAGKRVPDERDAADYQTMFAANEGAVAAPTAGLHFTPALETALRQRGVGIHRVTLH
                     VGAGTFLPVKVEDTSAHKMHSEWGTISRDTSDALNTARAKGGRIVAVGTTSLRLLESA
                     TARDGTIEPFAGETAIFITPGYGFRAVDILLTNFHLPRSTLFMLVSAFSGLDTMKRA"
BASE COUNT         1194 a         1985 c         1963 g         1176 t
ORIGIN      
        1 agctgatgtg gaagtcctcg tgtatcaaaa gatcgaaagc ataaagcggg cttagcgcgg
       61 caagcacgcc cggcgcccgc acaatgccgt ggactccaag cagtgcaatc accacgaacc
      121 aggcgagcat gaccggcccg aagatattgc cgataaaacc tgcgcccttt ttctggacga
      181 tgaaaagccc gatcaggatg gcgatggtca gcggcaccac cgcgggcgcc agcgaaggcg
      241 catcgacctt caagccctcg atcgcgctca gcaccgagat cgccggcgtg atggcgccgt
      301 cgccgtagag cagcgcggcg ccgacaaggc cgacgaccag tagctgcgca cgccacgtcc
      361 ccggccgggc attgcgggcg gaaagcagcg ccagcagcgc gacgatgccg ccttcaccgc
      421 ggttgtcggc gcgcaggatc aacagcgcat atttcagcga aatgatcagg atcagcgccc
      481 acaggatcag cgataccacg ccgaggatcg cttcaggggc aattggcccg ccatgggcgg
      541 ccgctctggc ggcctccttc agcgcgtaga ggggactggt accgatatcg ccatagacga
      601 cgccaagcgc tccgatcgtt gccgacagcg gaagcgcgct ggaaccgggt gatgttgcgg
      661 cgggaacggc agtcactggc ggagcccccc aagggtgtcc aagggtgtcc aagggtgtcc
      721 acggatatcg cgcccgtttg ctagaggggc gcgatcgaat gccagtctgc gacgcggcgc
      781 cggaaattac tatgagattt gtctggttcc ggaaagcagt cgcggttacg gcgcgcggtc
      841 ggcggttacc agccgggctt ccttgatggt cacggtcttt ttgccggctg cgcgcaggca
      901 ggatgcttcg aagttcggcc aggccttctg cgaacagttc gggcccacgg tgtggatatc
      961 cagccggtcg gctttggcca acggtacggg cacgctggct tcgacctgag gggcaaaccc
     1021 gggaagaacg gtgagtgcgg cggcgacaaa cgcagcaata gctattgctg aaagagcctt
     1081 gatcatgacg gtcccctgtc actaggcctt tacggcccgt cgtttcgtta accatttgat
     1141 aaccagcgtc ggtttccgga tgtcttcgcc gattgccaga atggtttcgt cccgcctgag
     1201 ttttgtgtcg tcgcccattc cggacgaaac acagccggct cgcatagaag acgcgccgac
     1261 cccgcaagtc gttcagacgc ctgtgaaata taaaaacccg ggtattagcc tcggaaccgg
     1321 gaaccagtcc ggcttgccgg gccgctccgg gcgcggtaga gggggcctat gccccgcatc
     1381 gcgctttatc ccggttcctt cgatcccgtc actgatggcc acctcgacgt ggtccggcac
     1441 gctgtcggct tgtgtgaccg gctggttgtc gccatcgggg tcaattccag taaaaagccg
     1501 atgttttcga tcgacgagag gttggagatg gtcgaggagg tatttgcccc ggtagccgcc
     1561 gccgccggct gcgccttcga ctgcacgacc tacgacaacc tcacggttac cgccgcgcaa
     1621 aagcatggcg cgaccatcat gatccgtggc ctgcgcgacg gcaccgacct cgatttcgag
     1681 atgcagatcg ccggcatgaa cgaaaccatg gcgcctgacg tgcataccgt gttcgttccg
     1741 gcctcggcgg cggtccgccc gatcaccgcc acactggtgc gccaaatcgc aaccatgggc
     1801 ggcgacgtct cggcattcgt gccgccatcg gtcgctgccc gcctcaaggc caaattcaag
     1861 ggctagctga cacgctcgcc ggcattctct gacctcattc cctgacttgg agttttcatg
     1921 atccgaattc tagctattgt tggtgcgctg atgtgcgcgg cccccgcgat cgcacagccg
     1981 ctgcctgcca atctcgacaa gccgaatgcg atcgtcatcg acaccaccaa gggccgcatc
     2041 gtcctcaagc tgcgcaccga cctcgcgccg aagcatgccg agcggatcaa gcaactggcg
     2101 cgggacggct attacaacaa cgtgccgttc catcgcgtca tcgaaggttt catggcgcag
     2161 accggcgacg gcaagaattt caacggaacc ggcggctcga aatatccaaa tcttcctgct
     2221 gaattctcca gcgtgccgtt caagcgcggt atcgtcggca tggcgcgtgc cagtgatccc
     2281 aactcggcga attcgcagtt cttcatcatg ttcgccgacg gttcgtccct gaatggccaa
     2341 tacacggtga tcggcgaggt ggtgtcgggc atggatgtgg tcgataagct caagcgcggt
     2401 gagccggtca ccgatcccga caagatggtg aaggtgcagg tcgcatccga cctcaagtga
     2461 gcggcggtgg cctggcgtcg ccaaccgctg acatggctgg caggggcgat gctgttcgcc
     2521 tcatcgagtt tgatggcgca ggcccagccg gcgcaggtca ataccctcaa ggaggtattt
     2581 gccaagctcg ggagctgctg gaagccgccg ccggcgtcac aagccaatcc cggaatcgac
     2641 atcacggtga tcgtcagttt caaccgcgcc ggcgagatcc ttggccatcc gaagatcacc
     2701 tatgaatcgg aacaggccac cgacaatgac cggctggcgt accgcgtcgc cgtgatggag
     2761 gcattgcaac gctgcacgcc gatgccattt accgagacaa tggccggcgc cgttgccggc
     2821 cggccgttcg cggttcagtt tcgcaatcgg aaatctccac ccaaacccat agagaagaga
     2881 gcatggctga caccgaaaac actttgatcc ttgaaaccac ccaggggccg gtcacgatcg
     2941 aaatgcggcc cgatctcgca cccggtcacg tcgcccggat caaggaactg gtacgcgagg
     3001 gattctacga tggtatcgtg ttccaccgcg tgatcgaagg cttcatggcc cagacaggat
     3061 gtccgcaggg aaccggcacc ggcggctccg gcaagaagct cgaggccgag ttcaacaagg
     3121 aaccgcatgt gcgcggcacc acctcgatgg cgcgcgcctc cagcccggat tccggcgaca
     3181 gccagttctt catctgcttc gacgatgcct cgttcctcaa caaccagtac acggtctggg
     3241 gcaaggtgac cgagggcatg gaaaacgtcg acaagatcaa gcgcggcgag ccggtacaaa
     3301 accccgacaa gattgttaag gcccgcatgg cgctcgacga cgcggcttga gtttcgtgcg
     3361 ctgaatttct tcacctctcc ccgccgggga gaggtcgcga acgaagtgag cgggtgaggg
     3421 gtcttgctcc cacatgatcc gtactccctc acccggagcc ttcgctgcgc tcaggctccg
     3481 acctctcccc acgggagagg tgaaggaccg tgcttcacag tcctggctca cgcgaggacg
     3541 ttcgagtccc gaattccacg cgcaccgacc tcttcgactt cgagcttccc gccggccaca
     3601 tcgcgctgcg cccggcgaac ccgcgcgatg gcgcgccaat gctggaaacg catcgcgcca
     3661 tgcgcaagag cctttgccct ttcaagcagt ggacgcttac ttcgccgaga gctgacggac
     3721 cgcacccagc caatcctcga tatggtagga gcggactatc ttgccgcctt caagcgtgtg
     3781 aacgtcgatc gacatcagct tgaatgactt gccggtgtga ggcgtcccca tgaattcgcc
     3841 cgccggcgta ccggttgctt cgccgcgcac ggtaacctga ttccccgaga ccaggatctc
     3901 cttgatttcc catttcaggt ccggaacgga tttgagacgt tgtccgatcc cggcgatgac
     3961 ttcatcgcgg ctgttgcaga catcgttgcc ccggcaggag atccactgcg gcgcggtcga
     4021 ttgcttgatg agttcggggc tgtctttggc gaattcggcg ttcaaggctt tgtagaacgg
     4081 cgcgacgctg tcgcgtgccg cctcagtgct gatttcagcc cgggcggaag atgccatcaa
     4141 gcctatggcg gcaacagcgg cgcagatggc gaaaattcgt ttcatggggg ctccggtgct
     4201 tgattgaggg ggcaccggtg atatgaaccc cattcgataa tcgatccaat gagtaatcat
     4261 agcccatcat gaatgaaatc gatttacgtc gcttcgacct caacctcttg gtcgtgtttg
     4321 aagtcttgat ggccgaacgt aacgtcaccc gggcggccga gcggctggga cggacgcaat
     4381 cggctgtcag tcattcgctg tcgcgattgc gcagccagtt gggcgatccg ctgctgatca
     4441 agggcggcgt ccggatgcag ccgacggcct tcgccatcga tctcatggag caggctcggc
     4501 caatgctgcg ggggatcgag cgcgtgcttt cgccgcgaca tgttttcgat ccggcatgtt
     4561 cgcagcgggt tttccggttg gcggcccccg atttcgcgtc gaccctgttc acaagcctgc
     4621 ttgcacgact acggtcggac gcgcccggtg tttccgtcga gtggacgaca gcgcgagagc
     4681 ccacgctgct ggacgtggcg gaggggcaag tcgacgtcgc gattgtgccc gcccaacttc
     4741 gcatgcctga tggcgtcaac agcgaagcca tcggggcgct gaaatggcga tgcttcggcc
     4801 gtcagggaca cccggcgttt tccaaatggg ggcgcaagag ctggtcgcac tggccgcatc
     4861 tcgccgtacg tgtcggcgac agcctgacga gcccggtgaa tctcgcggcg ggcgcggcca
     4921 ggctggagcg aaggatcgcc ggctgggtgc cgcatttctc cgccatcgct cccattctgg
     4981 ctggttcaga tttgctggcc accttgcctt cgattgcgat ggccgcgacg ttgcatccct
     5041 accggctgga cagcagagag gttccatttc cgatcgcgcc gctcccgcac gcgatgattt
     5101 ggagcaccgg ccgaagcagc catcccgagg tgaggtggct gcgcgatcgg ttgcgacctg
     5161 tcgtcaaaag caaatttacc gatctcgccg gttagaagga tcccgcgcga gacccccctg
     5221 cgtttgacgg tcgaaatgtc cgtgcggcgt gctacaccgg cggcccgtca tgcgacgcgc
     5281 tcaggtgcat cgcgaagatg atcaagtccc ggacaagcat gcgcaccgac ctcttcgact
     5341 tcgagcttcc caccggccac atcgcgctgc gcccggcgag cccgcgcgat ggcgcgcgga
     5401 tgctgatcgt gcagcccgac ggcgttttgc gtgacgacat cgtggccgat cttccgcact
     5461 ggcttgagcc cggcgatcag ctggtggtga acgataccag ggtgatatcg gcccagctga
     5521 gtggccgccg catcggccgg gaaaccgagc cgaagatcga ggcgacgttg atcaagcgcc
     5581 tcgatggctc gcgctggcag gcgctggtca ggcctgccaa aaaactgtcg agaggcgatg
     5641 tggttcgctt cggccatgag ggcagggtat gcctgctcgg ccatctggac gcgcaggtcg
     5701 aacacaaggg cgacgatggc gaggtgacgc tgtcgttttc atttcacggc cccgcgctcg
     5761 attccgccat cgccgatctg ggcaggccgc cgctgccgcc ttacatcgcc ggcaagcgcg
     5821 tgcccgacga gcgcgatgcc gccgactacc agaccatgtt cgcggccaac gaaggcgcgg
     5881 tcgcggcccc gaccgcggga ctgcatttta cccccgcgct ggagacggcg ctgcgccagc
     5941 ggggcgtcgg gattcacagg gtaacattgc atgtcggggc agggaccttt ttgccggtca
     6001 aggtggaaga tacgagcgct cacaaaatgc attccgaatg gggcaccatt tcccgcgaca
     6061 catcagatgc gctgaacaca gcaagggcca aaggtggccg cattgtcgcc gtcggcacga
     6121 cctcgctgcg gctattggaa agcgccaccg cgcgagatgg cacgatcgag ccgttcgccg
     6181 gggaaaccgc gatcttcatc acgccgggtt atggttttcg cgccgtcgat atcctcctga
     6241 ccaattttca cctgccgcgc tcgacgctgt tcatgctggt gtcggccttc agcggcctcg
     6301 acaccatgaa gcgggcct
//
LOCUS       DASWGM010000351         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_441663,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000351 DASWGM010000000
VERSION     DASWGM010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441663"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VGA15_10215"
     CDS             complement(<1..718)
                     /locus_tag="VGA15_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9588104.1"
                     /translation="MTTSLRLRAACVSIALFLAFVGIWHIATRGTGTVATMSPEYARL
                     MGLTATQGKSAMPGPFDVGAKLWEHLKRPFYDNGPNDKGLGIQLGYSIARVGLGYFLA
                     VMVAIPLGFLIGMSPLINKALDPFIQILKPISPLAWMPLALYTIKDSGLSAIFVIFIC
                     SVWPMLLNTAFGVAAVRKEWINVARTLEVGTVRRAFTVILPAAAPTILTGMRISIGIA
                     WLVIVAAEMLVGGTGIGYFVW"
     gene            complement(912..2297)
                     /locus_tag="VGA15_10220"
     CDS             complement(912..2297)
                     /locus_tag="VGA15_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9588105.1"
                     /translation="MSTFDDPFDADRKLSRSGCVCGQHRFAAEHDHATLMLRCEPVPT
                     EEQRYEGVVASAVMRAMFPKDAARRAFLKSVGASTALAAISQFFPIKTATEVFAEERA
                     PEKKDLKVGFIPITCATPIIMAAPLGFYAKHGLNVEVVKTAGWAVIRDKTLNKEYDAA
                     HMLAPMPIAITLGLGANAIPFTVPAIENINGQGITLAMKHKDKRDPKDWKGMKFAIPF
                     DYSMHNYLLRYYLAEHGLDPDTDVQLRSVPPPEMVANLRADNIDGFLAPDNICQRAIY
                     DGVGFLHILSKEIWDGHPCCSFAASREFITGSPNSFAALTRAIVDATAYASKSEHRKE
                     IAEAIAPANYLNAPVTVLEQVLTGTYADGLGDIKTDAKRVDFDPFPWQSFAVWMLTQM
                     KRWGQIKGDVDYKQVAEQVYLATDTRKVMTDMGLAPPETSYKSFSVMGKTFDPAKPED
                     YLASFKIRKAS"
     gene            complement(2421..>2613)
                     /locus_tag="VGA15_10225"
     CDS             complement(2421..>2613)
                     /locus_tag="VGA15_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9588106.1"
                     /translation="ENTPVYLYCFKGARASNTFLALKNAGVKDVRMYFGSWNEWSRDP
                     SLPIEEGLPTEAKLTGKAA"
BASE COUNT          502 a          806 c          817 g          488 t
ORIGIN      
        1 tccagacgaa gtagccgatg ccggtgccgc cgaccagcat ctcggcggcg acgatcacga
       61 gccaggcaat gccgatcgag atccgcatgc ccgtcaaaat cgtcggcgcc gccgccggca
      121 ggatcacggt gaaggcgcgc cggacggtgc cgacttccaa cgtccgcgcg acgttgatcc
      181 attccttgcg cacggcggca accccgaacg cggtattgag cagcatcggc cacaccgagc
      241 agatgaaaat gacaaaaatc gcggacagcc cggaatcctt gatggtgtag agcgcgagcg
      301 gcatccaggc cagcggcgag atcggtttta aaatttgtat gaacgggtcg agcgccttgt
      361 tgatcaaggg cgacatcccg atcaggaagc cgagcggaat cgcgaccatc acggcgagaa
      421 aatagccgag cccgacgcgg gcgatcgagt agccgagctg gatacccagg cccttgtcgt
      481 tgggaccgtt atcgtagaac ggtcgcttga ggtgctccca caattttgcg ccgacatcga
      541 acggacccgg catcgcggat ttgccttgcg tcgcggtcag ccccatcaac ctggcatatt
      601 cggggctcat ggtggccaca gtgccggtgc cgcgcgtggc gatgtgccag ataccgacga
      661 aggcaaggaa cagcgcgatc gacacgcaag ccgcgcgaag gcggagggag gtggtcatcg
      721 gatacggccc gtcagcgagt cgtcccggcg aacgccggga cccataaccc ccgccgctgc
      781 attttgctga aggcatctgc cccaatgccg aaacgcgaat ccgcgccgcc gcgacgagcg
      841 caatttgcgc tcacgctggg gtcccggcgt gcgccgggac gaccggtgca aatgtattcg
      901 cgccctcgct gtcacgacgc cttcctgatc ttgaagctcg cgagataatc ctccggcttc
      961 gccgggtcga aggtcttgcc catcaccgag aatgacttgt acgacgtctc cggcggcgcc
     1021 agccccatgt cggtcatgac cttgcgggtg tcggttgcga gatagacctg ctccgccacc
     1081 tgtttgtaat ccacatcgcc cttgatctgg ccccagcgtt tcatctgggt gagcatccag
     1141 accgcgaagg attgccaggg gaacggatcg aagtcgacgc gtttggcgtc ggttttgatg
     1201 tcgcctaatc cgtcggcata ggtgccggtc aaaacctgct ccagcacggt gaccggcgcg
     1261 ttgagatagt tcgccggcgc gatggcctcc gcgatctcct tgcggtgttc ggatttcgag
     1321 gcgtaggcgg tggcatcgac gatcgcccgc gtcaaggccg caaaactgtt gggcgatccg
     1381 gtgatgaact cgcggctcgc ggcaaaactg cagcagggat gaccgtccca gatctccttg
     1441 gacaggatgt gcaggaaacc gacgccgtca tagatcgcgc gctggcagat attgtcggga
     1501 gcgaggaagc cgtcgatatt gtcggcgcgc agattggcga ccatctccgg cggcggcacc
     1561 gaacgcagtt gcacgtcggt atcgggatcg aggccgtgct cggcgagata ataccgcagc
     1621 agataattat gcatcgagta gtcgaacgga atggcgaatt tcatgccctt ccaatccttc
     1681 gggtcgcgct tgtccttgtg cttcatcgca agcgtgatgc cctgcccgtt gatgttctcg
     1741 atcgccggca ccgtgaaggg gatggcattg gcgccgagac ccagcgtaat cgcgatcggc
     1801 atcggcgcca gcatatgggc ggcgtcgtat tccttgttga gggtcttgtc gcggatcaca
     1861 gcccatccgg cggttttgac gacctcgacg ttgagaccgt gttttgcgta gaacccgagc
     1921 ggtgccgcca tgatgatcgg cgtcgcgcag gtgatcggaa taaagcctac cttcaaatct
     1981 ttcttctcgg gcgcgcgctc ttccgcgaaa acctcggtcg ccgtcttgat cgggaagaat
     2041 tgcgagatcg ccgcaagcgc cgtcgaggcc ccgaccgatt tcaggaaagc ccggcgcgcg
     2101 gcatccttgg gaaacatcgc gcgcatgacc gctgaagcga cgacgccctc gtagcgctgc
     2161 tcttccgttg gaaccggctc gcacctcaac atcagtgtcg cgtgatcgtg ctcggctgcg
     2221 aaccgatgct ggccgcaaac gcagccggac cgcgacagct tgcgatcggc atcaaacggg
     2281 tcgtcgaagg tggacatgcg ccattccttt gcgtggtgcc gagctaacca aaaggcaaaa
     2341 cccgcccgcg cccgcggctt caccgcccgg ccgcgtggcg cggccgggcg atgaaaaaac
     2401 atgcactttt tatgccgctg ctatgccgcc ttgccggtca gcttggcctc cgtgggcagg
     2461 ccttcttcga tcggcagcga cggatcccgg gaccattcgt tccacgagcc gaaatacatc
     2521 cggacatcct tcacgccggc gtttttgagc gcgaggaagg tgttcgatgc ccgcgctccc
     2581 ttgaagcagt aaagatagac cggggtgttc tcc
//
LOCUS       DASWGM010000352         3936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1409373,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000352 DASWGM010000000
VERSION     DASWGM010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409373"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VGA15_10230"
     CDS             <1..1060
                     /locus_tag="VGA15_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9588107.1"
                     /translation="KTVPRKSGDPVVFTASLDKGAIKATSGGWAREITTRGLPIATDI
                     AGAHLFMNAGGAREMHWHNSAEWAYIVDGHCQVTVVDPESVAEVVNLGPGDLWYFPKG
                     HGHAIQTLGPAACHAILAFDDGLYSEHGTFGISDWMSRYDTEALSQAFGVSAEAFAQI
                     PKAETYIMQGEVLPLDGPQARSARELDRARTHRYALMAQKPRVSTPGGALYIASSKEF
                     PMSTTLTGMVLKLKRGAMHEPHWHTDANEWHYVLKGRTRVTLFAPDKRVAVAELSPGD
                     CAYIPRNCGHSIQSIGAEDAEIVGVLDSSTYHESSLSDWLAKAPRHLLANNFGMPEKS
                     IANFNKKRMVISAAATPT"
     gene            1186..1491
                     /locus_tag="VGA15_10235"
     CDS             1186..1491
                     /locus_tag="VGA15_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEX9588108.1"
                     /translation="MALLLVATSATWAADQTITLRLGIGSPLGLERSFKTVLIGDPNV
                     VDVHTRNDRSVMLEPLNPGATNLIFVDAKSIAITNVRILVCGAGAIPIKYQDGADCE"
     gene            complement(1549..2562)
                     /locus_tag="VGA15_10240"
     CDS             complement(1549..2562)
                     /locus_tag="VGA15_10240"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HEX9588109.1"
                     /translation="MLEKFERYPLTFGPTHIEKLERLGAHLGGKVELYAKREDCNSGL
                     AFGGNKLRKLEYIIPDAIASNADTLVSIGGVQSNHTRMVAAVAAKIGMKCRLVQESWV
                     PHEDAVYDRVGNILLSRIMGADVEMVDEGFDIGIRESWEAAIADVKAKGGKPYAIPAG
                     ASMHKYGGLGYVGFAEEVRAQEKELGFAFDYIVVCTVTGSTHAGMLVGFARDGRERKV
                     IGIDASFTPAQTKAQVLEIARNTAKLVELGKEIVEDDVILVEDYAYPIYGVPSQETKD
                     AIRLCARLEGMITDPVYEGKSMQGMIDLVKKGYFPEGSKVLYAHLGGAPAINGYAYAF
                     RNG"
     gene            2725..3189
                     /locus_tag="VGA15_10245"
     CDS             2725..3189
                     /locus_tag="VGA15_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX9588110.1"
                     /translation="MRGEIDRIGLKMLRLLQGNGRLTNAELAQLVDVSPATCHRRTQA
                     LFKQGYISRVRAMIAPQKVDRGALVMVGVVLDRSTPESFAAFEKAVAKLKFILDCHLV
                     AGDFDYFLKIRAEDIADFKRIHGDQLIALPGVRQTRTFFVMKEVVDNAPLEF"
     gene            3286..>3936
                     /locus_tag="VGA15_10250"
     CDS             3286..>3936
                     /locus_tag="VGA15_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9588111.1"
                     /translation="MTTTEMMQAAVLDSPGAAFRVAPMPRPHPKPGQVLVRVEASAVN
                     PLDTKIRAGQAAHARQPLPAILGIDLAGVVEAVGSGVTSFRNGDEVFGMTGGVGGLQG
                     SLAEYAAVEADLLAPKPASLGMRDAAAVPLIFITAWEGLVDRAAVHSGQKILIHGGAG
                     GVGHIAVQIARAFGAEVFATGSAASKTFIERLGAIPIDYRNMSVEDYVAQYSGGRGF"
BASE COUNT          775 a         1198 c         1168 g          795 t
ORIGIN      
        1 gaagacagtt ccgcgcaaat ccggtgatcc cgttgtcttc acggcttccc tcgacaaggg
       61 agcgatcaag gcaacgtccg gcggatgggc gcgtgaaatc accacgcgcg gacttccgat
      121 cgccaccgat atcgcgggtg cgcatctgtt catgaatgcg ggcggcgccc gcgagatgca
      181 ctggcacaat tcagcggagt gggcctacat cgttgatggc cattgccagg ttaccgtggt
      241 cgaccctgaa agcgtggccg aggtcgtcaa tctcggtccg ggcgatctgt ggtatttccc
      301 caaaggacat ggccatgcga tccagaccct cggtccggcg gcgtgtcacg cgatccttgc
      361 ctttgacgat gggctctatt cggaacacgg aacgttcggc atcagcgact ggatgagccg
      421 ttacgataca gaggcactct cacaggcctt cggcgtctcg gcagaagcgt tcgcccagat
      481 tcccaaggcc gagacctaca tcatgcaggg cgaggtgctg cctctcgatg gtccgcaggc
      541 gcgttccgcc cgcgagttgg atcgcgcgcg aacccatcgt tatgccctga tggcgcagaa
      601 gccgagagtg agcacgcccg gaggcgcgct ctacatcgcg tcgtcaaagg aatttccaat
      661 gtcgacgacg ttgacgggaa tggttctgaa actgaagcgt ggggcgatgc acgagccgca
      721 ttggcatacc gacgccaatg aatggcatta tgtgctgaag ggacgaacgc gggtcaccct
      781 cttcgcacct gacaaacgcg tcgccgtggc cgagctttca ccgggcgact gcgcctacat
      841 cccgcgcaat tgcgggcact ccattcaaag catcggtgcg gaagatgccg agatcgtcgg
      901 cgtactggac agcagcacct accacgagag ttcattgtcg gattggctgg cgaaggcgcc
      961 gcgtcacctg ctggccaata atttcggcat gcccgaaaaa tccatcgcga acttcaacaa
     1021 gaaacgcatg gtgatctccg cagcggctac cccgacgtga cgttggcctg cttggccgaa
     1081 atccggccag tcttatcgcg gcgtttcgtc cgcacaggtc gggggcttca acgcatcgga
     1141 ggaatcacaa tgaccgcaaa ccttgcacgc ctattcgccg ctgcgttggc gctgctgttg
     1201 gtcgctactt ccgcaacgtg ggccgcggat cagacgatca ccctcaggct cggtatcggc
     1261 tcgcccctcg gtcttgagag atccttcaaa accgtcctga ttggcgatcc gaatgtcgtc
     1321 gatgttcata cccgcaacga ccggtcggtc atgctcgagc cactgaatcc cggagccaca
     1381 aacctcatct tcgtcgacgc caaaagtatc gcgatcacca acgtcagaat cctggtttgc
     1441 ggcgcaggcg cgatcccgat caagtaccag gatggagccg attgcgagtg aattgacctg
     1501 aggcgaacgc cgcgggcatg tcgcaaggat catggccggc gttgccgctc acccgttgcg
     1561 gaatgcatag gcgtagccgt tgatcgccgg cgcgccgccg agatgggcgt agagaacttt
     1621 tgacccttcc gggaaatagc cctttttcac gaggtcgatc atgccctgca tggatttacc
     1681 ttcatagacg ggatcggtga tcatgccttc gaggcgtgcg caaagccgga tggcgtcctt
     1741 cgtctcctgc gacggaacgc catagatcgg gtaggcataa tcctcgacca gaatgacatc
     1801 gtcctcgacg atctccttgc cgagttcgac gagttttgcg gtgttccggg cgatctcaag
     1861 cacctgagcc ttggtttgcg ccggcgtgaa agacgcgtct atgccgatca ccttgcgctc
     1921 ccggccatcc ctggcaaagc cgaccagcat gccggcatgg gttgagccgg tgacggtgca
     1981 gacgacgata tagtcgaagg caaagccgag ttccttctcc tgcgcgcgca cctcttcggc
     2041 aaagccgaca taaccgagac cgccatattt gtgcatcgag gcgccggccg ggatcgcgta
     2101 aggcttgccg cccttggcct tcacatccgc gatcgccgcc tcccagcttt cccggatgcc
     2161 gatgtcgaat ccctcgtcga ccatctcgac atcggcaccc atgatccggc tcaagaggat
     2221 gttgccgacg cgatcataga ctgcgtcctc gtgcggcacc cagctttcct ggaccaaccg
     2281 gcatttcatt ccgattttcg ccgcgaccgc ggccaccata cgggtgtggt tcgactgcac
     2341 gcccccgatc gagaccagcg tatcggcgtt ggaggcaatc gcatcgggaa tgatgtattc
     2401 gagcttgcgc agcttgttgc cgccgaaggc cagtcccgaa ttgcagtcct cgcgtttggc
     2461 gtagagctcg accttgccac ccagatgggc gccgaggcgc tccagcttct cgatatgcgt
     2521 gggtccgaaa gtgagtggat aacgttcgaa tttctccagc atgcgagatg cccttctgtc
     2581 agccgcttcg atggcttgga aattaacatt gtcggtcaga aaggtgcttt ctaagacatc
     2641 cggtaaattt actacttcga atatcttctg tataaaacat gtggttatct tcggatattt
     2701 cacaaagata cggaagattc tcccatgcgc ggcgaaattg accggatcgg cctgaaaatg
     2761 ctgcgcctgc ttcagggcaa tgggcggctc accaatgccg aactggcgca gctggtcgac
     2821 gtcagtcccg cgacctgtca ccgccggacc caggcgctgt tcaagcaagg ttacatcagc
     2881 cgcgttcgcg cgatgatcgc gccgcaaaag gtcgacaggg gcgcgctggt gatggtcggc
     2941 gtcgtgctcg atcgctcgac accggagagt ttcgcggcgt tcgaaaaggc cgtcgcgaaa
     3001 ttgaaattca tcctcgattg tcacctggtc gcaggcgatt tcgattattt cctgaaaatc
     3061 agggccgaag acatcgcgga tttcaaacgg attcacggcg accagttgat cgcgctgccc
     3121 ggtgttcggc aaaccagaac gttctttgtg atgaaagagg tcgtcgataa cgcgccgctt
     3181 gaattttgat ccattgatct gttgcgccga gccgcgcgcc gttgtcattt tccaaacaag
     3241 ccaaatatac cgggctacgt ctcagcaacg ggaggcagac ccgccatgac aacaacggaa
     3301 atgatgcagg ccgcggtgct cgacagtcct ggtgctgcgt tccgggttgc gccgatgccc
     3361 cggccgcatc ccaaaccggg gcaggttctg gtccgggtcg aggccagcgc agtcaacccg
     3421 ctcgacacca agatccgcgc cggccaggcc gctcatgcgc gccagccatt gcccgccatt
     3481 ctcggcatcg accttgcggg cgtggtcgaa gcggtcggtt cgggcgtgac aagctttcga
     3541 aacggcgacg aggtttttgg aatgacgggc ggtgtcggcg gcctgcaagg gtcgctggcg
     3601 gaatatgccg ctgtcgaggc cgatctgctc gcgccaaaac ccgccagtct cggcatgcgt
     3661 gacgccgcgg ccgttccgct gatcttcatc accgcgtggg aaggactggt cgatcgcgct
     3721 gccgtgcatt ccgggcaaaa gatcttgatt catggcgggg ccggcggcgt cggtcacatc
     3781 gccgtccaga tcgcccgcgc cttcggagcg gaggtgtttg caaccggctc ggccgccagc
     3841 aagactttca tcgaacggct cggcgcgatc ccgatcgact atcgaaacat gtcggtcgaa
     3901 gactatgtcg cccaatacag cggcggccgc ggcttc
//
LOCUS       DASWGM010000353         7881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2254926,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000353 DASWGM010000000
VERSION     DASWGM010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2254926"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..614
                     /gene="fdxA"
                     /locus_tag="VGA15_10255"
     CDS             276..614
                     /gene="fdxA"
                     /locus_tag="VGA15_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX9588112.1"
                     /translation="MTYVVTEACIKCKYTDCVEVCPVDCFYEGDNMLVIHPDECIDCG
                     VCEPECPADAIKPDTEPGLEKWLEVNTEYAKTWPNITQKKESPADAKDFEGMEGKFEK
                     YFSPAPGTGD"
     gene            1031..1867
                     /locus_tag="VGA15_10260"
     CDS             1031..1867
                     /locus_tag="VGA15_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX9588113.1"
                     /translation="MPNKTAKTAAKATGSKASASKVAPAKTPPNKTPPNKTPPNKTAP
                     KPVAKVAAKPVVAAKPLVAKPVAAAPRVEEPKKVLTQRQGFKTNEFVVYPAHGVGQIL
                     AIEEQEIAGAKLELFVINFMKDKMTLRVPTAKVANVGMRKLSEPALVKRALETLKGRA
                     RVKRTMWSRRAQEYEAKINSGDIVAIAEVVRDLYRSESQPEQSYSERQLYEAALDRLS
                     REIAVVQHGTETEAVKEIEGQLAKSPRRGAKAETEVEADGEADVDADADGDDAAVADE
                     AA"
     gene            complement(2084..3535)
                     /locus_tag="VGA15_10265"
     CDS             complement(2084..3535)
                     /locus_tag="VGA15_10265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9588114.1"
                     /translation="MSRRLWAAPMLLCAAFALAACSDVAKFQTPIPTASPLKARTVAQ
                     TPAVEREHERILSAYGGSYDDPNLEALIGKTVDRLVAASDRPDQAYKVTILNSGAVNA
                     FALPTGQLYVTRGLIALASDTSELSSVLSHEMAHVLAKHAAIREDQARQAAVVTRVVT
                     DMGNDPDLTALALAKTKLTMASFSRAQEFEADAIGVSISARAHFDPYGAARFLTSMER
                     NAELKAGKTSLDPRAQDFLSSHPATPERVENAQASARQYTSPASGERDRETYLSAIDN
                     IVYGEDPSEGFVRGRRFLHPKLGFTFAAPENFTLDNTAQAVIGVREGGAQAMRFDVVR
                     VPAEQSLGDYLNSGWMENVDKASTEDLTINGFPAASAQARGDQWQFKVYALRFGSDVY
                     RFIFATKEKTTESDRNARETVSSFRRLSLEEIQAARPLRIKIITAQPGDTVESLSHRM
                     AAVDRPTERFRVLNGLDAHAQVKVRDLVKIVVD"
     gene            complement(3596..4405)
                     /locus_tag="VGA15_10270"
     CDS             complement(3596..4405)
                     /locus_tag="VGA15_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX9588115.1"
                     /translation="MEPATRAEPRRGGFVAVALPCGLLLVAAAAGPADAAGCAFEPQG
                     EGRVAAVIDARSFRLQDGREVRLAGIEPVAVQKENANRPSALAAIVIGRDVTLRGEDD
                     TPDRYGRQPAFVFLDGSEASVQGLLLTRGEALVSATVADKDCALSLMAAETAARDAKR
                     GTWADPAAIKNAESPGDILTGIGRFTVVEGKVLSVRQAGATTYLNFGRSWTQGFAVTI
                     SRRATAALEIAGMVVKSLENRRIRVRGWVEARGGPRIEVLRAGQIELLGEN"
     gene            4601..4909
                     /locus_tag="VGA15_10275"
     CDS             4601..4909
                     /locus_tag="VGA15_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9588116.1"
                     /translation="MDTDAVYYCVGGRTAVAIRAPAAEHAGTAFEQNRVGLHHLCFRA
                     RERADVDELHGFLCALGVTIVRAPREDQWAPGYYSLLFEDPDGIRLELNHVPGKGLLG
                     "
     gene            5104..6309
                     /locus_tag="VGA15_10280"
     CDS             5104..6309
                     /locus_tag="VGA15_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588117.1"
                     /translation="MNRVLSGVFASALVLAATAAMAQSKPPLKLGGILDMSGLYADIT
                     GPGSEIAANMAAEDFGGEVLGRKIEIIAADHLNKADLAANIARDMLDNQGVEMIFDVA
                     ASATALAAGEIAKTRNKIVIFNGPGSIRLSNEACGPYTVHYVFDTFAQANVTGLAAVK
                     QGLDSWFFLTADYAFGQDLEKDTTNVVRKSGGKVLGSVRHPLNTSDFSSYLLQAQSSK
                     AKVIGLANAGGDTINAIKQAAEFGLTKSGQKLSPLLAFVTDIDSVGLETAQGLLLAEA
                     FYWDLNDDTRAFSKRFMARVKRVPTAAQVGVYSSVTHYLKAVKQAGTTDSAVVMKIMK
                     DTPINDVFATNGRIREDGRMVHDMYLFEVKKPSESRGRWDDYKLLATIPGNEAFQSLE
                     LSRCPLVKK"
     gene            6516..7308
                     /locus_tag="VGA15_10285"
                     /pseudo
     CDS             6516..7308
                     /locus_tag="VGA15_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713636.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
     gene            7310..>7881
                     /locus_tag="VGA15_10290"
     CDS             7310..>7881
                     /locus_tag="VGA15_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588118.1"
                     /translation="MASYMRLRQICLVAPHLEPVISDIAGIMGLHVCYRDGNVAKYGL
                     ENALLPVDTILLEVVAPFQPGTAAGRFIDKTGGRGGYMAIFCCDDPDERGEHAKAIGV
                     RVANVIDHAPYHGVQLHPRDCRAAFIEFNHTDGSDDILGPYPPAGPDWQKSIAKDVTR
                     ALIGVEMESPEPRALAEHWGRIIGIPVTQN"
BASE COUNT         1565 a         2470 c         2412 g         1434 t
ORIGIN      
        1 gacgtagatc tgctgccgta aaatcatccc gaagctcaac cgctgcgctc atttgttgct
       61 ccccgaatca aatcgtgaag cccaaatgaa tcacaacccg ccgaaaactc cgagagtcag
      121 tccctacgca atctggtata actatctgta ttcgggcgaa ttcagctcgc gccgaagcgc
      181 ctgaaaacac cgcttttcag tgctctgcgt cacttgcgac cacgcagttc ctgcgctacg
      241 caaaccactg aaattgaccc gttccggagt cttggatgac ttacgtcgtc actgaagcct
      301 gcattaaatg caaatacacc gattgcgttg aggtctgccc cgtagattgc ttctacgaag
      361 gcgacaacat gctggtcatc catccggatg aatgtatcga ctgcggtgtc tgcgagcccg
      421 aatgtcccgc cgacgccatc aagcctgata ccgagcctgg actggaaaaa tggctcgagg
      481 taaataccga atatgccaag acttggccga acatcaccca gaagaaggaa tccccggccg
      541 acgccaagga ttttgagggg atggaaggca aattcgagaa atatttttct ccggctccgg
      601 ggaccggcga ctgatccgat acgatgctta tcggcttgag cggcggccct ttttggcctt
      661 attcaacgac aaacggctaa ccctaatacc tttgatatcg ccgaaactac cctgcggagc
      721 ccccgaacgg ggcgtaaatc attgattttc acggaaaatg tgctatattg gccatattca
      781 agccgaaccc agccgtgcgc gtacttgtgg cctcaggggg ttcccgtgaa aacatcgaac
      841 aggggcgtgg cagttccgcg cgcaggctgt gtcacagaaa acgcgtaaaa agagtgcttc
      901 caaagattct aaaaagtctg ctgcggccag ccgtggcgca accaaaagcc gcgtccggtc
      961 ttctccaagg gatacccgga aggccaccag ggcctcggct gcaaaatcct cgaaaaacaa
     1021 aagaagcgca atgccaaaca agactgcgaa aactgccgcg aaagcgaccg gttcgaaggc
     1081 ctctgcttcg aaggttgctc ccgccaagac tcctccgaac aagactcctc cgaacaagac
     1141 tcctccgaac aagactgctc cgaagcccgt cgcgaaggtt gcggcgaagc ccgtggtcgc
     1201 cgccaagcct ttggtcgcga agcccgtggc tgctgcgccg cgggtcgaag agccgaagaa
     1261 ggttttgacc caacgtcagg gcttcaagac gaatgaattc gtggtgtatc ccgcgcacgg
     1321 cgttggccag atcctggcga tcgaagagca ggagatcgcc ggcgccaagc ttgagctgtt
     1381 cgtcatcaat ttcatgaagg acaagatgac gctgcgggtg cccaccgcca aggttgccaa
     1441 tgtcggcatg cgcaagctgt cggaaccggc cttggtcaag cgcgcgctgg aaaccctcaa
     1501 aggccgcgcg cgcgtcaagc gcacgatgtg gtcgcgccgg gcgcaggaat atgaagccaa
     1561 gatcaattcc ggagacattg tcgcgatcgc cgaagtggtg cgcgatctgt atcgctcgga
     1621 atcgcagccc gaacagtcct acagcgaacg tcagctctac gaagcggcgc tcgatcgcct
     1681 gtcgcgggaa atcgccgtcg tgcagcacgg gacggaaacc gaggcggtga aggaaatcga
     1741 aggccagctc gccaagagcc cgcgccgcgg cgccaaggcg gagaccgaag tcgaagcgga
     1801 cggtgaagcc gacgtcgatg ccgatgcaga tggtgacgac gcggcggtcg cggacgaagc
     1861 ggcgtaaggc agcttcggaa ggttcagata ttcaaaagcc cggtcgatga gaccgggctt
     1921 ttttgttggt tgtgatttag cgaagtctat caacacgtcg tcccggcgac cgccgggacc
     1981 catacgccgt gtgctatcgt tctggcattg gagcagaggc cttcttcaac aatcgacgcc
     2041 ggtgactatg ggtcccggcg ttcgccggga cgacgatggt ggatcaatcc accacgatct
     2101 tcacgagatc ccgcaccttc acctgggcat gggcgtcgag gccgttgagg acgcgaaaac
     2161 gctccgtcgg ccggtcgacc gcggccatgc ggtgcgacag cgattcaacg gtatcgccgg
     2221 gctgcgcggt gatgattttt attcgcagcg gtcgcgccgc ctgaatttcc tccagcgaca
     2281 atcggcggaa cgaactcaca gtttcacgcg cgttgcggtc gctctcggtg gtcttttcct
     2341 tggtggcgaa aatgaagcgg tagacgtcgc tgccgaagcg cagcgcatag accttgaact
     2401 gccattgatc gccgcgcgcc tgagcggatg ccgccgggaa gccgttgatc gtcagatcct
     2461 cggttgacgc cttgtcgaca ttttccatcc agccggaatt gagatagtcg ccgagcgatt
     2521 gttcggccgg caccctcacc acatcgaagc gcatcgcctg ggcgccgccc tcgcgcaccc
     2581 cgatcacggc ctgtgcggta ttgtccagcg tgaagttttc aggagcggcg aacgtgaagc
     2641 cgagcttggg atgcaggaag cgccggcccc ggacgaagcc ctcgctcggg tcttcgccgt
     2701 agacgatgtt gtcgatcgcg gacagatagg tctcgcgatc gcgttcacca ctcgccggcg
     2761 aggtgtactg ccttgcgctc gcctgggcgt tctccacgcg ctccggcgtt gccgggtgcg
     2821 acgacagaaa atcctgggcg cgcggatcga gcgaggtctt tcccgccttc agctcggcgt
     2881 tgcgctccat cgacgtcagg aaacgcgccg cgccgtaggg atcgaaatgg gcgcgcgccg
     2941 agatactgac gccgatagcg tccgcttcga attcctgggc gcgggaaaag ctcgccatcg
     3001 tcagcttggt tttggcgagc gcaagggcgg tgagatcggg atcgttgccc atgtcggtga
     3061 cgacgcgggt gacgacggcg gcctgacgcg cctggtcctc gcggatcgcc gcgtgtttgg
     3121 ccagcacatg ggccatctcg tggctcagca ccgaggacaa ttccgaggta tcgctggcca
     3181 gcgcgatcag gccacgcgtc acgtaaagct gtccggtcgg aagcgcgaac gcattcaccg
     3241 cgcctgaatt gaggatcgtc accttgtagg cctggtcggg acggtcggat gccgcaacca
     3301 gccgatcgac cgtcttgccg atcagggctt ccaggttcgg atcgtcatag gaaccgccat
     3361 aggcggacag aatccgttcg tgctcgcgct cgacggccgg ggtttgcgcg accgtccggg
     3421 ccttgagcgg gcttgcggtc gggatcgggg tttggaactt ggcgacatcg ctgcaggcgg
     3481 cgagcgcaaa agccgcgcac agcagcatgg gcgcagccca aagccggcgg ctcatccagg
     3541 tcttgtgccg tcccgcgcat tctgccacgt cagctactcg tatttgtcca gaaagctaat
     3601 tctcgcccag caactcaatc tgccccgcgc ggagcacttc gatccgcggc ccgccgcgcg
     3661 cctcgaccca gcctcgtaca cgaattcgtc gattctcgag agatttaacg accataccgg
     3721 caatctcaag cgccgcggtc gcgcgccttg aaatagtcac agcaaagccc tgtgtccagc
     3781 tccgtccgaa attcaggtaa gtcgtcgccc cagcctgtcg taccgacaaa accttgccct
     3841 cgaccaccgt gaatcgcccg atccccgtca agatatcgcc cggactttcc gcgtttttta
     3901 tggcggctgg atcggcccag gtgcctcgtt ttgcgtcgcg agcggccgtt tccgcggcca
     3961 tcaggctcaa ggcacagtct ttatccgcca cggtggccga aaccagcgcc tcgccccgcg
     4021 tcagcagcag gccctgcacc gacgcctcgg agccatccag aaacacgaag gcgggctgac
     4081 ggccgtagcg gtcgggcgtg tcgtcctccc cgcgcaacgt cacatcgcgc ccgatcacga
     4141 tggccgccag cgccgatggg cggttggcat tttctttttg gaccgcgacc ggctcgatgc
     4201 cggcgaggcg gacctcgcga ccgtcctgca ggcgaaagct gcgcgcatcg atcacggcgg
     4261 cgacgcggcc ctcaccctgc ggctcgaacg cgcatccggc tgcgtcagcc ggaccggccg
     4321 cggcggcaac aagcaacaag ccgcacggca atgctaccgc gacaaatccg ccgcgccgcg
     4381 gttccgctct ggtcgccggt tccatgactc tacgtccggt cgaatttctg tatcttgacc
     4441 atctgccaac cagctccgta tccgatatcc tttcgctgac aaagggggaa gtcgatggaa
     4501 gtcaacggca tcgcgcatat tttcctgacc gcatcgaatt tcgaacccac cccgcgaatt
     4561 ctaccgcaag ctgctgccgt ttcttgggct gaagcctgtc atcgacaccg acgccgtcta
     4621 ttattgcgtc ggcggtcgca ccgcggtcgc gatccgagcg ccggcggccg agcacgccgg
     4681 cactgcgttc gagcagaatc gcgtcggcct gcaccatctg tgttttcgtg cccgcgagcg
     4741 cgccgacgtt gacgagttgc acggcttcct gtgtgcgctc ggtgtgacga tcgttcgcgc
     4801 gccgcgcgag gatcagtggg cgccgggata ttattcgctg ctgttcgaag atcccgacgg
     4861 catcaggttg gagctcaacc atgttccggg aaaggggctt ctgggctgag cggcatgcaa
     4921 attcggccgg gctggcgatg aggcgatgat cattccgctt tgcggaaaat gcttggaatt
     4981 caaaagcgca gtgcggggcc gcgcgggtga ctgcgcttgc cgctgcggat cgcttgcggc
     5041 atgatcgcgg caacagaaat cgaggccgct ggcgaatgcg gcgcgttcgc gagggaggct
     5101 gcaatgaacc gcgtactgtc cggtgttttc gcatcggcgc tggtgcttgc cgccaccgcg
     5161 gccatggcgc aaagcaagcc gccgctgaaa ctcggcggca tcctcgatat gtcaggtctc
     5221 tacgccgaca tcaccgggcc gggcagcgag atcgccgcga acatggcggc cgaggatttc
     5281 ggcggcgagg tgctgggccg aaagatcgaa atcatcgcgg ccgatcatct caacaaggcc
     5341 gatcttgccg ccaatattgc ccgcgacatg ctcgacaatc agggcgtcga gatgatcttt
     5401 gacgtggcgg cctccgcgac cgcgctcgcg gccggcgaga tcgcgaagac gcgcaacaag
     5461 atcgtgatct tcaacggtcc gggatcgatc cgcctcagca acgaagcctg cggtccctat
     5521 accgtgcact atgtgttcga cactttcgcg caggccaacg tcacgggtct cgccgccgtc
     5581 aagcagggcc ttgacagctg gttcttcctc accgccgatt acgccttcgg acaggatctg
     5641 gaaaaagaca ccaccaatgt cgtgcggaag tccggcggca aggtgctggg cagcgtccgc
     5701 cacccgctca acacctcgga tttttcgtcc tacctgctgc aggcgcaaag ctcgaaagcc
     5761 aaggtgatcg gattagccaa tgccggcggc gacaccatca acgcgatcaa gcaggccgcc
     5821 gagttcgggc tgaccaagag tggccaaaaa ctgtcgccgt tgctggcctt cgtcaccgat
     5881 atcgacagcg tcggtcttga gaccgcgcag ggcctattgc tcgccgaagc gttttattgg
     5941 gacctcaacg acgacacccg cgctttctca aaacgcttca tggcacgcgt caagcgggtg
     6001 ccgaccgcgg cccaggtcgg cgtctattcg tcggtcacgc attatctgaa ggcggtgaag
     6061 caagccggca ccaccgactc ggccgtcgtc atgaagatca tgaaggacac cccgatcaac
     6121 gacgtgttcg ccacgaacgg gcggattcgc gaggacggcc gcatggtgca cgacatgtat
     6181 ctgttcgagg tcaagaagcc gtcggaatcc aggggccgct gggacgacta caagctgctg
     6241 gcgaccatcc ccggcaacga ggcgttccag tcgctcgaac tgtcacgctg tccgctggtg
     6301 aagaagtagc cgcacgccgt cattgcgagg agcgtaagcg acgaagcaat ccatccttcc
     6361 gcagtgctcg ctatggattg cttcgcggag cctgtcatcg ggcggcgcgg cgcctgtcat
     6421 cgggcgcgca ttcgcgcgac ccgttggcgc cgacccggtg gctcgcaatg acggctttga
     6481 aacacaacga ataacaacac aaagggcaaa aacccatgaa cgacatcgtc ctgcaaaaac
     6541 tcgaccaggg cctgctcacc atcaccatga accgccccga tcggcgcaac gcgctcaatc
     6601 cggacatgac gcggggtctg gtggaagcgg cgcggcgcgc ggcggaggac cacgaggtcc
     6661 gcgcggttct gctcaaaggc gccggcggca ccttctgcgt cggcggcgat gtgaaatcga
     6721 tggcggaggg ccgggcgccg ctgccgttcg aagccaagat ggccaatctc cggcgcggca
     6781 tggaagtctc ccgcattctg cacgacatgc cgaagcccgt ggtggcgcaa gtcgatggcg
     6841 cggccgctgg cgcgggctta tcaattgcgc tggcctgcga tctccgcgtc gcctctgctt
     6901 ccgtcaagat caccaccgcc ttcgccaagg tcgggctgtc cggcgattac ggcggcacct
     6961 attttctcac tcatctgctc ggcagcgcca aggcgcggga gctttacttg atgtcgccgg
     7021 tgctgtcagc gcaggaagcc ttcgcactcg gcatcgtgac aaaagtcgtg cccgacgccg
     7081 aggtcgaggc ggcggcgcat gagctcgcga tgtcgctggc gcagggcccg tcggtgacgc
     7141 tcggttacat caagcgcaac atcaacaacg ccgagcacct gtcgctggaa gcctgtttcg
     7201 acgccgaggc gatccatcac tcgcgcgccg gcgagcgaga ccgccgacca caaggaagcg
     7261 gcaaaagcct tcgtcgaaaa gcgcaagccc atctttcagg ggcattgagg tggcgagcta
     7321 tatgaggctg cggcagatct gcctggtggc gccgcatctc gaacccgtca tctccgatat
     7381 cgccggcatc atgggcctcc acgtctgcta tcgcgacggc aatgtcgcca aatatggcct
     7441 ggagaacgcg ctgctgccgg tcgataccat tttgctggaa gtggtggcgc cgtttcaacc
     7501 gggcacggcg gcgggacgct ttatcgacaa gaccggcggc cgcggcggct atatggcgat
     7561 tttctgctgc gacgatcctg atgagcgcgg ggaacatgcc aaggccatcg gcgtgcgcgt
     7621 cgccaacgtc atcgatcacg cgccctatca cggcgtgcaa ctgcatccgc gcgattgccg
     7681 cgcggcgttc atcgaattca accacaccga cggcagcgac gacattttgg gtccctatcc
     7741 gccggcaggt ccggactggc agaaatcgat cgctaaggat gtgacgcggg cgctcatcgg
     7801 cgtggagatg gaaagtccgg agccgcgggc gttggccgaa cactggggca ggatcatcgg
     7861 cattcccgtc acgcaaaatc a
//
LOCUS       DASWGM010000354         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1700822,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000354 DASWGM010000000
VERSION     DASWGM010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700822"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGA15_10295"
     CDS             complement(<1..463)
                     /locus_tag="VGA15_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588119.1"
                     /translation="MIVITGLDPVIHLRTKAFCEDGWMPGSSPGMTSAFVSPTIPQIR
                     RRDFTISRREAPELCKNLSPPWRAWGMPGARCTRSPLCNVESTGVEATGTPESPGIPA
                     RNGFNGFLRALPGDRALLPPSSADMVLSKPGRADLTSAKLDASVGASGPHDF"
     gene            722..1876
                     /locus_tag="VGA15_10300"
     CDS             722..1876
                     /locus_tag="VGA15_10300"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX9588120.1"
                     /translation="MHKGRVVFGAMDEVVFGRPAADAIVEQMDRLRADRAFLMVSGTL
                     NRQTDEIKKIRKALGVRCAAIFDTMPPHTPREAVIAAAAQARVANVDLIVTVGGGSIT
                     DGAKAVQLCLANNISTVEGIDTIRANKGVAPQLTAPSVRQISVPTTIAGGEFSAIAGV
                     TNQRTKVKEMLRHDLVMPRAAILDPAVTVHTPEWLFLSTGIRAVDHCVEGLCAREAHP
                     YADAQAMKGLSMLAQALPRVKADARDLDARMDCQIGTWLSMGALSAGIPMGASHGIGY
                     VLGAAFGVPHGYTSCVMLPSVMRWNKPDNGERQELVSAAMGHPGEDAAEVLDWFIRDL
                     GMPRSLHAVKIGPEHFEAIAEQAMATPWIPRNPRKIDGPAQVREILLLAA"
     gene            1893..>2387
                     /locus_tag="VGA15_10305"
     CDS             1893..>2387
                     /locus_tag="VGA15_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9588121.1"
                     /translation="MYTGKHAHLRPLQPAFIMANSGEAVSYAELEARSNRLAHLFRNR
                     GLKRLDHYAIFMENNSRYLEACGAGERSGLYFTCVNSYLTGSELAYIVNNSESRILIT
                     SMAKLDVAREALEECPKVELCIVVDGPSEGERIVNLLEATSGLPKTPISDECVGTAML
                     YSSGT"
BASE COUNT          471 a          755 c          742 g          419 t
ORIGIN      
        1 cgaagtcgtg tggtcctgac gccccgacgc tggcgtcaag ttttgcggag gtcaagtcgg
       61 cccgaccggg cttagacaag accatatccg ctgacgacgg tggcaaaaga gcccggtcac
      121 cggggagagc acgaaggaaa ccgttaaaac cattgcgtgc gggaatgccg ggtgattccg
      181 gtgtacctgt ggcttctacc cctgtgcttt ctacattgca cagggggctg cgggtgcagc
      241 gggcacccgg cattccccac gccctccaag ggggcgagag atttttgcac aactcgggcg
      301 cttcgcgccg cgagatcgtg aagtcgcgtc tgcggatctg cggaatggtg ggcgacacaa
      361 atgcgctcgt catgcccgga cttgatccgg gcatccatcc atcttcgcaa aaagcctttg
      421 ttcgaagatg gatcaccggg tcaagcccgg tgatgacgat catgatgggt gcgacgacgc
      481 aatccagcaa tgacggtatc aagacaggag cactcagctt gccgcactca cagctctctt
      541 accttgctga aagcaagcaa cgacaccccc cggaacttct cgtcaaggat cgatgtgcgg
      601 gtgaccggcg gctcggcgcg cttgcgcgtc gatcattttt tggttcccct ccccttgttc
      661 actccgctag attgctgcca aagctgcaac aaaacaaaat cgtcctggga ggacaatcgt
      721 catgcacaag gggcgtgtcg tattcggcgc catggacgag gtcgtgttcg gacggccggc
      781 cgcggacgca atcgtcgagc agatggaccg gctgcgcgcc gaccgcgcgt ttctgatggt
      841 gagcggcacg ctcaaccggc agaccgacga aatcaagaag attcggaaag ccctcggggt
      901 ccgttgcgcg gccattttcg acacgatgcc gccgcacacg ccgcgcgagg ccgtgatcgc
      961 tgccgccgcg caggcccgtg tcgccaacgt cgatctcatc gttacggtgg gcggcggctc
     1021 gatcaccgac ggcgccaagg cggtgcagct ttgcctcgcc aacaatatca gcaccgtcga
     1081 gggcatcgat acgatccgcg ccaacaaggg cgtcgcgccg cagctaaccg caccatcggt
     1141 acgtcagatc agcgtgccga cgacgattgc cggcggagag ttcagtgcca tcgccggcgt
     1201 caccaaccag cgcaccaagg tcaaggagat gctgcgccat gacctcgtga tgccgcgcgc
     1261 ggcgatcctc gatcccgccg tgaccgtgca cacgccggaa tggctgtttc tgtcgaccgg
     1321 catccgcgcc gtcgaccatt gtgtcgaagg cctgtgcgcg cgggaagccc atccctacgc
     1381 cgacgcccag gcgatgaagg gactgtcgat gctggcgcag gcgctgccgc gcgtgaaggc
     1441 cgacgcccgt gatctcgacg ccaggatgga ctgccagatc ggcacctggc tctcgatggg
     1501 agcgttgtcc gccggcatcc cgatgggcgc cagccacggc atcggttatg tgctcggcgc
     1561 cgcgttcggc gtgccgcacg gatatacatc ctgcgtgatg ctgccatcgg tgatgcgctg
     1621 gaacaagcct gacaatggcg agcggcagga actggtatct gctgcgatgg gacaccccgg
     1681 cgaggacgcc gccgaggtgc tggactggtt catccgcgac ctcggaatgc cgcgcagcct
     1741 gcacgccgtc aaaatcggcc cggagcattt cgaggcgatc gcggagcagg cgatggcaac
     1801 gccatggatt ccgcgcaacc cgcgcaagat cgatggcccg gcgcaagtgc gcgagattct
     1861 tcttctggcc gcatgagaac attggagaca cgatgtacac cggcaagcat gctcatctcc
     1921 gcccgcttca gcccgctttc atcatggcga actccggcga agccgtgagc tatgccgaac
     1981 tcgaggcgcg cagcaaccgt ctggcgcatc tgttccgcaa tcgcggcctg aagcggctcg
     2041 accattatgc gatcttcatg gagaacaaca gccgctatct cgaagcctgc ggcgccggcg
     2101 agcggagcgg attatacttt acgtgcgtga attcatatct gaccggaagc gagctcgcct
     2161 acatcgtcaa taacagcgag tcgcgcatcc tgatcacgtc gatggcaaaa ctcgacgtcg
     2221 cgcgcgaggc cctcgaggaa tgccccaagg tcgagctctg catcgtcgtc gatggtccca
     2281 gcgaaggcga acgcatcgtc aatctgctgg aggcgacgag cggactgccc aagacgccaa
     2341 tatcagacga atgcgtcggt accgcgatgc tctattcgtc cggcacc
//
LOCUS       DASWGM010000355         9339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_302657,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000355 DASWGM010000000
VERSION     DASWGM010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302657"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGA15_10310"
     CDS             complement(<1..218)
                     /locus_tag="VGA15_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588122.1"
                     /translation="MVGHQRTASKTRKTLASTGKISMLQHETASLASGRPCPDRSRAR
                     ISGKTALKVIASSIRKGNVIEQDGKLYVV"
     gene            207..1259
                     /gene="epmA"
                     /locus_tag="VGA15_10315"
     CDS             207..1259
                     /gene="epmA"
                     /locus_tag="VGA15_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEX9588123.1"
                     /translation="MADHDQLSPWWSATRHADIRPFLAARSAITKAIRAWFDEQGFAE
                     VETGILQVSPGNETHLHAPRTELTSATGALATRYLRTSPEFACKKLLAAGEPKIFEFA
                     RVFRDRERGDLHLPEFTMLEWYRADAAYDAVMADSVVIIAHAAQATGIGRFSFRGRSA
                     DPFAEPELLTVAAAFERFAGIDLLATIAGGEGDRAGLAAAARTRVRIADDDTWSDIFS
                     KVLVEHIEPNLGQGRLTVLFEYPAPEAALARAKTSDPRVAERFEIYACGVELANGFGE
                     LTDAEVQRRRFMQAMDEKERRYGERYPLDEDFLDAVAKMPEASGVALGFDRLVMLAGG
                     ALRIDQVVWTPPAGEA"
     gene            1256..2386
                     /locus_tag="VGA15_10320"
     CDS             1256..2386
                     /locus_tag="VGA15_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HEX9588124.1"
                     /translation="MSKINLKPAMTLREPAELVAHGLAPAADLADLEQVAARYAVAVT
                     PDIAALIDMDDSDDPIARQFIPRAEELAVQPGESADPIGDHTHSPVAGIVHRYPDRVL
                     FKLVHVCAVYCRFCFRREMVGPGKATALSSAAYRGALDYIREHREIWEVILSGGDPLM
                     LSPRRLAEIMGDLAAIDHVKIIRIHTRVPVADPARISAEMIAALRVKGAATWVALHAN
                     HAKELADKARAACAAMIDAGIPMVSQSVLLRGVNDNAAALEALMRAFVECRIKPYYLH
                     HGDLAPGTAHLRTTLAEGRELMRALRGRVSGLCQPEYVLDIPGGHGKAPVGPNYLSHA
                     NSFSETNSFSEQRERQAETRYRIVDYCGDVHLYPPEPSLSDD"
     gene            2461..2721
                     /locus_tag="VGA15_10325"
     CDS             2461..2721
                     /locus_tag="VGA15_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588125.1"
                     /translation="MRKLIMSMALLALIGAPAALAQTGSKTGTSGAANQQPPPFREAP
                     IGHRQPRADDVPSEKNLSNPNTPANREDAALDRKIKSICRGC"
     gene            complement(2851..3426)
                     /locus_tag="VGA15_10330"
     CDS             complement(2851..3426)
                     /locus_tag="VGA15_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588126.1"
                     /translation="MFDLDQFTADCRAALAADRSHRHVRDVVARAVADPISTLKGLGE
                     PKCAGVHTLYRSGDLTILNVVWAPMMTIMPHNHSMWAVIGIYTGREDNIFWRRLPGNP
                     GKVEAAGAKALCVRDAAPLGPDIIHSVTNPIPRLTGAIHVYGGDFFDPTHRSEWNPET
                     LLEGPFDPERAVRRFEEANASFANGQGHLKQ"
     gene            complement(3566..5194)
                     /locus_tag="VGA15_10335"
     CDS             complement(3566..5194)
                     /locus_tag="VGA15_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588127.1"
                     /translation="MAEFRSARVLFASTLIVLASQGLARAAEERLGEVNFPISCSPAA
                     QQQFNRGLAMQHSFFFPETVKAFTAIAEQEPSCAMAYWGIAISQRPNPLVGPFPGDVL
                     KRGWEAIEKARAASQKTEREIAWIEALAAYYQDYATVPQQKRTANYEAAMARLSARYP
                     DDAEAAIFYALALNEAADPVDKTYVRQLKAADILERLEAKYPDHPGIPHYIIHSYDYP
                     ELAMRGVFAASRCARLAASAPHALHMPSHIYSTLGMWQEAISSDRAADDETIAYTVRV
                     NPQAAADPAKNPGRYHFLDFLTNAYLQLGQDQQAKRIVDARNSVAEYPAAFRYSGHTA
                     FAAIPVRYAFERAAWVEATALAIPKTPFAQAEAIAWFGRAIGAARSGDMTKAREAIDQ
                     LGVLKDRLAKANDDYWTGQVVIQQQAAGAWPALAEGRKADAIAAMRQAADLEDRSGKT
                     VAMENRLSPIRELLGELLLDANEPAQALKEFEASLRNNPNRYRSFAGAAKAAERAGDR
                     AQARSYYEKLVALAAHADGARPDLVAAKQYLAGN"
     gene            5302..6246
                     /locus_tag="VGA15_10340"
     CDS             5302..6246
                     /locus_tag="VGA15_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9588128.1"
                     /translation="MNLRFLRTFVAIADNAGFARAAARLNLTQSAASRQIRSLEDELG
                     LRLFARIGRNVRLTSEGEDLLVRSRRLLFDAEALGQRASALKTGEVGVLRVGATPQVI
                     ENLLADFLSGYRKRHAGVEVHLVEDGGSRLPGRLERGDIDIAQMPAGEDRFSGRLLFP
                     MHVLAVLPQGHRLSRRAVLDVTELADEPLLRLGSSFASHGWFEAACQVAHIRPRVLLE
                     SVAPQTLIALARTGHGVAVVPSPVRIPRAGVRAAVVVHRGVSIGRWAVAAWDPQRFLP
                     PYAVQFVEELVAHCRRGYPGHEFSRRAPPLPRPREAND"
     gene            complement(6297..7382)
                     /locus_tag="VGA15_10345"
     CDS             complement(6297..7382)
                     /locus_tag="VGA15_10345"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX9588129.1"
                     /translation="MLSTTDDLRIEQIKELSTPAEVMREIPRTLTATRVVMAARNAIH
                     AILHGTDDRLLVVVGPCSVHDPAAAMDYAERLATLRERLVERIEIVMRVYFEKPRTTV
                     GWKGLVNDPDLDGSFNINKGLRLARSVLSAVNNLGLPAATEFLDMTTPQYIADLVAWA
                     AIGARTTESQIHRELASGLSCPVGFKNGTDGNVRIAAEAVKSASHPHHFMAVTKGGRS
                     AIAATSGNEDCHIILRGGSKPNYDSAGVASAAAELARAGVAQRMMIDTSHANSSKKPE
                     NQPPVIADIARQISNGEQRIVGVMIESNLVAGHQDVLTDTALTYGQSITDGCIDWNTT
                     VPALNLLADAVASRRDMLRRKCREYSA"
     gene            complement(7605..8177)
                     /locus_tag="VGA15_10350"
     CDS             complement(7605..8177)
                     /locus_tag="VGA15_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX9588130.1"
                     /translation="MTVPQDITKLQSEVSAAVREHWKAFLFEGILLVVLGLAAMILPP
                     LASLAVTIFLGWMFLISGVAGLALTFWARQMPGFWWSLVSAVLAVLAGIVLLTRPVQG
                     VLTLTIVIGAYFLAEGVTTIMYALEHRRELSERWSWLLFAGVLDILIAAMIITGLPGS
                     AEWAIGLLVGINLLFGGASLIGMALAARKG"
     gene            complement(8279..9316)
                     /locus_tag="VGA15_10355"
     CDS             complement(8279..9316)
                     /locus_tag="VGA15_10355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX9588131.1"
                     /translation="MNLLASLPTVIGTAAVAPALLILWLVIAAAERPGPPAKVWTAFL
                     LGAASISLLGIARAPFASILAAPENPWVAQALHSIFGVAAPEETVKILVIVAVSTRRR
                     AFAAPMDTVVYGAAAGLGFAAYENLAYLEQHTDMWRSLAALRSVLTVPFHGALGIIAG
                     AYLALGRAGTALGAHRHNRDWARISSRILMLFAPIALHAGFDFPLLTLQQNPDFDAST
                     RLLLGSASVLIGFSSIAFAARLVQRVARHQAPRTDIARERLSQLRRMWALLVVGGGAG
                     FAGLAFVLSSIHHWWVNPERNPALVLIPVGLVSIAVGIALLVVTTAIYILGRNRMRTT
                     SRDFSSAPGRS"
BASE COUNT         1722 a         2852 c         3016 g         1749 t
ORIGIN      
        1 accacgtaaa gcttgccgtc ttgctcgatg acgttgccct tgcgaataga actggcgatg
       61 actttcaaag ctgtttttcc tgatattctg gcccgggacc gatccggaca agggcgtccc
      121 gaggccagcg aagcggtttc gtgctgcaac atactgattt tgccagtcga tgccagcgtt
      181 ttgcgggttt ttgaggcggt tcgctgatgg ccgaccacga tcagctttcg ccatggtggt
      241 cggcgacacg gcacgccgac atcaggccat tcttggcggc gcgaagcgcc atcaccaagg
      301 cgatcagggc atggttcgac gaacagggtt ttgccgaggt cgaaaccggg attttgcagg
      361 tctcgccggg gaatgagact cacctgcatg cgccgcgcac cgaattgacc tccgccactg
      421 gcgcgcttgc gacgcggtac ttacggacct cgcccgaatt cgcctgcaag aaattgctgg
      481 ccgcaggcga gcccaagatc tttgagtttg cgcgggtgtt tcgcgaccgg gagcgcggcg
      541 atctccatct tcccgaattc accatgctgg aatggtaccg cgccgatgcg gcctatgacg
      601 ccgtgatggc cgatagcgtc gtcattatcg cccatgccgc gcaggcaacc ggcatcgggc
      661 gattttcgtt tcgcggccgc agcgccgatc catttgccga gccggaactg ttgaccgtgg
      721 cggcggcgtt cgagcgcttt gccggtatcg atctgctcgc caccatcgca ggaggcgagg
      781 gcgatcgcgc tggcctggcg gcagcggcca ggacacgggt gcggatcgcg gacgacgaca
      841 cctggtcgga tatcttcagc aaggtgctgg tcgagcacat cgaacccaat ctggggcagg
      901 ggcgcttgac ggtgttgttt gaatatccgg cgcccgaggc ggcgctggcg cgcgccaaaa
      961 cgtccgaccc acgcgtcgcc gagcgttttg agatttatgc ctgcggcgtt gaacttgcca
     1021 atggatttgg cgaattgact gatgccgagg tgcagcgccg gcgtttcatg caggccatgg
     1081 acgaaaagga gcggcgctac ggcgagcgct atccgctcga cgaggatttt ctcgacgccg
     1141 tggcgaagat gccggaagcg agtggcgtcg cgctcggctt cgaccggttg gtgatgctgg
     1201 ccggcggcgc gctgcggatt gatcaggtgg tgtggacgcc gccggccggt gaggcatgag
     1261 caagatcaat ctcaagcccg ccatgacgct gcgtgagccc gcagaactgg tcgcccacgg
     1321 cctggcacct gcggctgatc ttgccgatct ggaacaggtc gcggcgcgct atgcggtggc
     1381 ggtgacgccg gatattgccg ccttgatcga catggatgat tccgacgatc cgatcgcgcg
     1441 acaattcatt ccacgtgctg aggaattggc ggtgcagccc ggcgagagcg ccgatccgat
     1501 tggcgatcac acgcattcgc cggtcgccgg catcgtgcat cgctatccgg atcgcgtgtt
     1561 gttcaagctg gttcacgtct gcgcggtgta ttgccggttc tgttttcgcc gcgaaatggt
     1621 cgggccgggc aaggcgaccg cgctctcgtc cgccgcctac cgcggcgcgc tcgactatat
     1681 ccgagagcat cgcgagatct gggaggttat cctgagcggc ggcgaccccc tgatgctatc
     1741 gccacggcgg ctcgctgaga tcatggggga cctcgccgcc atcgatcacg tcaagatcat
     1801 ccggatccac acccgcgtgc cggtggcgga tcccgcccgc atcagcgcgg agatgatcgc
     1861 ggcgctcagg gtcaaggggg ctgcgacctg ggttgcactg cacgccaacc atgccaaaga
     1921 attggccgac aaagcccgcg ccgcgtgcgc tgcaatgatc gacgccggaa tccctatggt
     1981 cagccagtcg gtgctgctgc gcggcgtcaa tgacaatgcc gcggcgctgg aagcgctgat
     2041 gcgggccttt gtcgaatgcc ggataaaacc ctattacctg caccacggcg atctcgcgcc
     2101 cggcacggcg catctgcgca cgacgctggc ggagggccgg gaattgatgc gtgcattgcg
     2161 ggggcgggtt tccgggctgt gtcagccgga atacgtgctc gatattcccg gcggccatgg
     2221 caaggcgccg gtcggtccga attatttgtc acatgcaaat tctttttcgg agacaaattc
     2281 tttttcggaa caacgtgaac ggcaggcgga aacgcgctat cgtatcgtcg actattgcgg
     2341 cgacgttcat ctctatcctc cggagccatc attgagcgat gactgacgga ggaggggcag
     2401 gaccggagag gctcccgatc ctgccaggtc gatgcgtttc tctgttggtg gagaattcag
     2461 atgcgaaagc tgatcatgtc gatggcgcta ctggccctga ttggcgctcc agcggcgttg
     2521 gcgcagacgg gtagcaaaac cggaacatcg ggcgctgcaa atcagcagcc cccgcccttc
     2581 cgcgaggccc cgatcggcca ccggcaaccg cgcgccgatg acgtgccgtc ggagaaaaac
     2641 ctgagcaatc cgaacactcc cgccaaccgc gaggatgcgg cactcgaccg caagatcaag
     2701 agcatctgcc gcggctgctg aaacggccag ggcgtgtact cataagttca actccggcaa
     2761 tgcttggccg atgtccgggt tggcgcgtat gacggcgtta acgtcagaca tcgcgccagg
     2821 tccgaaaaac cgcggggaac ggacaatccg ttattgtttc aggtgccctt gtccgttcgc
     2881 aaaactagca ttcgcctctt cgaagcggcg aaccgcgcgt tccggatcga acgggccttc
     2941 cagcagggtt tccgggttcc actcgctccg atgcgtggga tcgaagaaat caccgccata
     3001 tacatgaatg gcaccggtta ggcggggtat aggattggtg acggagtgaa tgatgtcggg
     3061 tccgagcggc gctgcatccc tcacgcatag cgccttggcg ccggccgcct cgaccttccc
     3121 cgggttgccg ggcaggcggc gccaaaagat gttgtcctcg cgtccggtat agatgccgat
     3181 caccgcccac atggaatgat tgtggggcat gatagtcatc atcggcgccc atacgacatt
     3241 gagaatggtc aggtcaccgg accgatagag tgtgtggacg ccagcgcatt ttggctcgcc
     3301 caaccctttc agcgtggata taggatcggc gactgcccgc gcgacgacgt cgcgcacatg
     3361 cctgtgcgat ctgtcggccg cgagcgcagc ccggcaatcg gcggtgaatt ggtcgagatc
     3421 gaacatcgtc gtccctggtt cgatagcaaa cccgacctgg ccaattctct ctggcggtcg
     3481 gccgcatcat tgcgtttgcc gtggcgctag cagcaatagc aggcgcccgt aagcgacgac
     3541 agccagaaaa cggcgcgctc gctggtcaat tccccgcgag atactgcttc gctgcgacaa
     3601 ggtcgggccg tgcaccatcg gcatgggccg caagtgctac gagcttctcg tagtagctcc
     3661 gggcctgcgc tcgatcgccg gcccgttcgg cggcctttgc ggcgcccgca aacgagcgat
     3721 aacggttcgg attgttgcgc agcgacgcct cgaattcttt cagggcctgc gcgggctcat
     3781 tggcatcgag cagcaactcg ccgagcagtt cccggattgg cgagaggcga ttttccatcg
     3841 cgacagtctt gccgcttcga tcttccaggt cggccgcctg gcgcatcgcc gcaatggcat
     3901 ccgccttgcg gccctcggcg agcgcaggcc aggccccggc agcctgctgc tggatgacaa
     3961 cttgtccggt ccagtaatcg tcgttggcct tggcaagccg gtccttgagc acgccgagct
     4021 gatcgatggc ctcccgggcc ttcgtcatat cgccgctgcg ggcagcaccg atcgcgcggc
     4081 cgaaccaggc aatcgcctcg gcttgcgcga atggcgtttt cggaatggcc agcgccgtgg
     4141 cttccaccca ggcggcgcgc tcgaaggcgt agcggacggg gatggctgca aaggccgtgt
     4201 gcccgctgta acggaaggct gccggatatt cggcgacgct gttgcgcgca tcgacgatgc
     4261 gtttcgcctg ctggtcctgt cccaactgca ggtaggcgtt ggtcaggaag tcgaggaaat
     4321 ggtagcggcc agggtttttc gcagggtcgg cggcggcctg cggattgacg cgcaccgtgt
     4381 aggcgatggt ctcgtcgtcg gccgcgcgat ccgaactgat cgcctcctgc cacatgccga
     4441 gcgtggagta gatgtgcgag ggcatgtgta gtgcgtgcgg cgccgaagcc gccagtcgcg
     4501 cgcaacgcga agcggcgaac accccacgca tggcgagctc gggatagtcg tagctgtgga
     4561 tgatgtaatg cgggatgccg ggatggtctg gatacttcgc ctccagtctt tcgagaatat
     4621 cggcggcctt gagttgcctg acataggtct tgtcgaccgg atccgccgct tcgttgagcg
     4681 ccagcgcata gaaaatcgcc gcctcggcgt catcgggata gcgcgccgac agccgcgcca
     4741 tcgccgcttc gtagtttgcg gtgcgctttt gctgcgggac cgtcgcgtag tcctggtagt
     4801 aggcggcgag cgcctcgatc caggcgatct cacgctcggt tttctgtgaa gctgcgcgcg
     4861 ctttctcgat cgcttcccag ccacgcttga ggacatcgcc ggggaacgga ccgaccagcg
     4921 ggttagggcg ctggctgatg gcgatgcccc aataggccat ggcgcaagac ggctcctgct
     4981 cggcaatggc ggtgaacgcc ttcacggtct cgggaaagaa gaaggaatgc tgcatggcga
     5041 ggccgcgatt gaattgctgc tgcgccgccg ggctgcatga gatcggaaaa ttgacctcgc
     5101 caagccgttc ctcagccgcg cgcgcaagcc cctggcttgc cagcacgatc agcgttgagg
     5161 caaacagaac tcgcgcactc cgaaactcgg ccatgacgat tttcccgttt ggatttctat
     5221 cacgaaggct gtgcacgatc tcattgatgt gtctaattca tttattgcat gctaaatgat
     5281 gataaattgg aatgttttcc gatgaacctc cggttcctgc gcacgttcgt cgcgatcgcc
     5341 gacaatgccg gttttgcgcg cgcggccgcg cggctgaatc tgacccagtc ggcggcgtcg
     5401 cggcaaatcc ggtcgctcga ggatgaactg ggtttgcggc tgttcgcgcg gatcggacgg
     5461 aacgtgcggc tgacgtcgga gggcgaggac ttgctggtgc gcagccggcg gctattgttt
     5521 gatgccgagg cgctcggcca gcgcgccagc gcgctcaaga ctggagaggt cggagtgctg
     5581 cgggttggtg ccacaccgca ggtcatcgag aatctgctgg cagattttct gtcgggttat
     5641 cgcaaacgcc acgccggcgt cgaggtccat ctggtggagg atggtggctc gcgcctgccg
     5701 ggccgcctcg agcgcggcga catcgacatc gcccaaatgc ctgccggcga agatcgcttc
     5761 tcggggcggc tgctctttcc gatgcacgtt ctggccgtgc tgccgcaggg tcaccggctg
     5821 agccgtcgtg ccgtgctcga cgtgacggag ctcgccgacg aaccgttgct gcgtttgggc
     5881 tcaagcttcg cgtcgcatgg ctggttcgag gccgcctgcc aagtcgcgca cattcggccg
     5941 cgggtactcc tcgaaagcgt cgcgccgcag acgctgatcg cattggcgcg gaccggccac
     6001 ggcgttgccg tggttccgtc tccggtacgg attccccgcg cgggggtccg ggcggcggtg
     6061 gtggtgcatc gcggcgtgtc gatcggacga tgggcggtcg ccgcctggga cccgcagcgc
     6121 ttcctgccac cctatgccgt gcaattcgtc gaagagctgg tcgctcattg tcgacgcggc
     6181 tatcccggcc acgaattcag tcgtcgcgct ccgccgctac cgcggccaag agaggcgaac
     6241 gactagacca agagaagagg ccgagcgccc gatgaccatc gatcagcata accgactcag
     6301 gccgaatact cccggcattt gcggcgcagc atatcgcgtc tcgacgcgac cgcgtcggcc
     6361 agcaggttta gtgctggcac ggtcgtgttc caatcaatgc agccgtccgt aatgctctgc
     6421 ccataggtca gcgccgtgtc ggtcagcaca tcctggtggc ccgccaccag attgctttca
     6481 atcatgactc cgacgatgcg ctgctcgccg ttggatatct ggcgggcaat atcggcaatc
     6541 acgggtggct ggttctccgg cttcttgctg ctgttggcgt ggctggtgtc gatcatcatc
     6601 cgctgagcca cgcctgcacg ggcgagttcg gccgcggcgg aagcgacgcc ggcggaatca
     6661 taatttggct tgctgccgcc acgcagaatg atgtgacagt cttcattgcc tgatgtcgcg
     6721 gcgatcgcgg aacgtccacc cttggtgacg gccatgaaat ggtggggatg cgatgccgac
     6781 ttcaccgcct cagcggcgat gcgcacattg ccgtcggtgc cgttcttgaa gccgaccgga
     6841 caggaaagtc cggaagcaag ttcgcgatga atttggcttt cggttgtacg cgcgccgatg
     6901 gctgcccacg caaccagatc ggctatgtat tggggcgtcg tcatatcgag gaattcggtc
     6961 gccgccggca gaccgagatt gttcacggcg gaaagcacgc tgcgggcgag acgcaggccc
     7021 ttgttgatgt tgaagctgcc atccaggtcg ggatcgttga cgaggccctt ccaccccacc
     7081 gttgtgcgcg gcttctcgaa atagacgcgc atgacaattt cgatccgctc gacgagccgc
     7141 tcgcgcaagg tagcgaggcg ctcggcgtag tccatggccg cggctggatc gtggaccgag
     7201 cagggaccga cgacgacgag aaggcgatcg tccgttccgt gaaggatcgc gtggatggcg
     7261 ttgcgcgccg ccatcacaac tcgcgtcgcg gtcaaggtgc gcggtatttc gcgcatgacc
     7321 tctgccggcg tactcagctc ttttatttgc tctattcgaa gatcgtctgt ggtgctcaac
     7381 acggctgtgg ctcctgtgtg ttttaaggac ctgccggcca aacaaaaagc cgccaagtcg
     7441 ggcggctgtt cggacttcct gctgcaattc ttcagattga gcgtgatcct cccgccgcca
     7501 gcgagctgtc gaagctaaaa taccaaaaat tgctggcggc aacggtgatc atggcacgcc
     7561 tatagcgcat gcattgtctg ttgtcatctc aaaatcgggc tggttcagcc cttgcgcgcg
     7621 gcgagcgcca ttccgatcag cgacgcgccg ccgaacaaaa gattgatgcc gaccagcagg
     7681 ccgatcgccc attccgccga acccggaagc ccggtgatga tcatggcggc aatcaggatg
     7741 tccaggacgc cggcgaacag cagccaactc caccgttccg acaattcgcg gcgatgttcc
     7801 agcgcgtaca tgatggtggt gacgccttcg gccaggaaat acgccccaat cacgatcgtg
     7861 agggtgagta cgccctgcac gggcctggtc agcagcacaa tgccggcgag aacagccagc
     7921 accgccgaaa ccagcgacca ccagaagcca ggcatttgcc gggcccagaa ggtgagcgcc
     7981 agccctgcga cgccgctgat cagaaacatc cagcccagaa agatggtgac ggcaaggctg
     8041 gcgagcggcg gcaggatcat ggccgcgaga cccaacacca cgagcaggat gccttcaaac
     8101 aaaaaggcct tccaatgctc gcgcacggca gcgctcactt cggattgcag cttggtgatg
     8161 tcctggggaa cggtcattgg aaaatctccc gatgctggta tcgaagaacc acaatatagc
     8221 ccccgccaac gccggccggt caaatttctt caggtcgttt aagcgaaacg gttctgtcct
     8281 agcttcggcc gggcgcggac gaaaagtccc gggacgtcgt gcgcatccga ttgcgaccga
     8341 gaatatagat cgccgtggtg accaccagca gggcgatgcc gaccgcaatc gaaacaaggc
     8401 ccaccggaat aagcacgagt gcggggttgc gctcggggtt tacccaccag tgatggatcg
     8461 acgacagcac aaaggccaga ccggcaaatc ccgcacctcc gcccaccacc aataatgccc
     8521 acatccgccg caactggctg agccgctcgc gggcaatgtc ggtacgcggc gcctgatggc
     8581 gggcgacccg ctgcaccagc cgcgcggcaa acgcgatcga gctgaagccg atcagcacgc
     8641 tcgccgatcc cagcagcagt ctggtggatg catcgaagtc cggattttgc tgcagcgtca
     8701 gcaatgggaa atcgaacccg gcatgcagtg cgatcggcgc aaacagcatc aggattcggc
     8761 tggagatgcg ggcccagtcg cggttatgcc ggtgcgcgcc gagcgcggta ccggctcgcc
     8821 ccagcgccag gtaggcgccg gcgatgatgc cgagcgcgcc atggaacggc acagtgagaa
     8881 cgctgcgcaa cgccgccagc gatcgccaca tatcggtatg ttgctcgaga taagcgaggt
     8941 tttcgtaagc cgcaaagcca agccccgccg ccgcgccata aaccacggtg tccatcgggg
     9001 ctgcgaaagc ccggcgacgc gtcgagacgg cgacgatgac gagaatcttg acggtttcct
     9061 cgggtgccgc gacgccaaat atcgaatgca gcgcctgcgc aacccagggg ttctcgggcg
     9121 ctgcgaggat cgaggcgaac ggcgcacgtg caatccccag cagcgagata ctggcggcgc
     9181 caagcagaaa cgcggtccac accttggccg gcggtccggg gcgttcggcg gcggcgatca
     9241 caagccacaa tatcagcagc gcgggtgcca ccgctgcggt gccgatcacg gtcggaaggg
     9301 atgcaaggag attcatctaa ggcagcagca ctttcaaaa
//
LOCUS       DASWGM010000356         1662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2113061,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000356 DASWGM010000000
VERSION     DASWGM010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113061"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGA15_10360"
     CDS             <1..268
                     /locus_tag="VGA15_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588132.1"
                     /translation="RIFLPLSLPGVLAGTTLVFVLCLGFYITPELLGGGRTVMVSMLV
                     SRNVELYNQWGAASAVGVVLLISVFVIFLIVGRFIPLDRILGQK"
     gene            265..1068
                     /locus_tag="VGA15_10365"
     CDS             265..1068
                     /locus_tag="VGA15_10365"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588133.1"
                     /translation="MKTFSPGRIALVLFCSLVLLYLILPVLIIVPMSFSSTRFLTFPP
                     PSLSLRWYQEYIGNAAWMQATRVTFAVAALTVLIATPLGVAAAYAISNSKLRIMRIIH
                     MMLMLPLVVPIIITAVGIFFVYAKVGLVATMSGLVLANVMLGLPYVITSVVAGLQSFD
                     PAQEMVARSLGMSRLRSFFAVTLPQIKASVLTGSIFAFISAMDETVVGLFISGGQYQP
                     LTKRMFTALRDEIDPTIAAISTLMTAASFILVLIASTRQKKTPDGGLPA"
     gene            1093..1239
                     /locus_tag="VGA15_10370"
     CDS             1093..1239
                     /locus_tag="VGA15_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588134.1"
                     /translation="MSDLPKLDRININILVQLQKDGRSATCCLQSTKAAAGVAARLGV
                     ARAD"
     gene            1323..1514
                     /locus_tag="VGA15_10375"
     CDS             1323..1514
                     /locus_tag="VGA15_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588135.1"
                     /translation="MTTPLEQAVAAFFPACGPISTRSPEPSPAIDPGFTISLRMPKFR
                     DAAFQQKYNGSLQVAGRPE"
BASE COUNT          277 a          525 c          495 g          365 t
ORIGIN      
        1 gcggatcttc ctgccgctgt cattgccggg cgtgctcgcc ggcacgacgt tggtgttcgt
       61 gctgtgtctg ggcttctaca tcacgcctga actgctcggc ggcggccgca ccgtcatggt
      121 atcgatgctg gtgagccgca acgtcgagct ctacaaccaa tggggcgcgg caagcgccgt
      181 cggcgtcgtg ctgctaatca gcgttttcgt aattttcctc atcgtcggcc ggttcattcc
      241 gctcgatcgc atattggggc aaaaatgaag acgttctcgc ccggccgcat cgccctcgtg
      301 ctgttctgtt cgctggtgct gctttacctg atcttgccgg tgctgatcat tgtgccgatg
      361 tcgttctcga gcacccgctt cctgacgttc ccgccgccgt cgctatcgct gcgctggtac
      421 caggaatata tcggcaatgc ggcctggatg caggcgaccc gcgtgacctt cgcggttgcg
      481 gcgctcaccg tgttgatcgc gacgccgctc ggcgtcgccg cggcctacgc catcagcaac
      541 tcgaagctgc gcatcatgcg aataatccat atgatgctga tgctgccatt ggtagtaccg
      601 atcatcatca ccgcggtggg catcttcttc gtctatgcca aggtcggact ggttgcgacc
      661 atgtcgggac ttgtgctggc caatgtcatg ctcggcctgc cctatgtcat cacctccgtc
      721 gtggcgggct tgcagagttt cgatcctgcg caggagatgg tggcacgcag tctcggcatg
      781 agccgcttgc gcagcttttt cgccgtcacg ctgccgcaga tcaaggcgag cgtgctgacg
      841 ggcagcatct tcgccttcat ctctgcgatg gacgagaccg tcgtgggcct gttcatctcc
      901 ggcggccagt accagccgct caccaaacga atgtttaccg cactccgcga cgagattgat
      961 ccgaccatcg ccgcgatcag cacgctgatg acagccgcat cgttcatcct ggtactgatc
     1021 gccagcacgc gacaaaagaa aacgcctgac ggcggtctcc ctgcctgaga ttgcgttact
     1081 cggagatcgc ccatgtctga ccttccaaaa ctggatcgta tcaacatcaa tatcctggtc
     1141 cagttgcaga aagacgggcg atcagcaacc tgttgcttgc agagcaccaa ggcggcggca
     1201 ggtgtagccg cccgccttgg cgtggcgagg gccgactagg cgccttcacg ccaggtacgc
     1261 tcttcggacc ggagctcgcc gtggagacgg gtcggagagt tcgttctcga ccgagcgcag
     1321 gcatgaccac tcccctggag caggcggtgg ctgccttttt tccggcgtgc gggcccatct
     1381 cgacgagatc gcccgaaccg tcgcccgcga tcgatcccgg cttcacaatc tctttgcgca
     1441 tgccgaagtt ccgcgatgct gccttccagc aaaagtacaa tggttcgctg caggtggcgg
     1501 ggcgccctga atgagcacgt agccgctttg gcctcgcgat gccggcaacg aatccgcggt
     1561 cgccggcgag gtgatcgaat agggtcgctt acttccgtga gcgctgcgaa atccgcctcg
     1621 ggcgcggtga aatcagtccc ggatgttgct acgctgatcc tg
//
LOCUS       DASWGM010000357         8188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1834677,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000357 DASWGM010000000
VERSION     DASWGM010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834677"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGA15_10380"
     CDS             complement(<1..417)
                     /locus_tag="VGA15_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9588136.1"
                     /translation="MDEPAAVKTSLQFPVPAQMKAWVLGNPDELFPRDKPTPVPGRAE
                     VLVRIDAVAICATDLQIIHGGSPASIQGGLPFNKNFTPGHEYMGTVAALGPSVDEFEI
                     GERVSVEIHAGCGQCKRCRQGMYTSCLNYGRAEKGHR"
     gene            complement(496..1407)
                     /locus_tag="VGA15_10385"
     CDS             complement(496..1407)
                     /locus_tag="VGA15_10385"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9588137.1"
                     /translation="MAIARGTDQGSTEKLGYVGLGMMGFPMTQRLVDAGHDVTVWNRS
                     SGKAAALVEAGARLASFPREVATNASIIFMCLTDAAAVEDVVFGPDGLATADGAGKLV
                     VDFSSIHPDAARSIAARLKLANGMGWIDAPVSGGTIGAEQGTLAVMAGGDAADIERAR
                     PYVLAMARRLTHMGPTGAGQTSKLCNQVIVGCAMAVLAEATRLAVNAGIDARRLPEAL
                     AGGFADSIPLQLFVPRMVQGIHSPPLGHIATMLKDLDTVIDVARDTSSPVPMSALAAQ
                     LFRLAKSARGADADALEIYKLSASQVG"
     gene            1595..2581
                     /locus_tag="VGA15_10390"
     CDS             1595..2581
                     /locus_tag="VGA15_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588138.1"
                     /translation="MMRIPSKWIVAAGAAVAVLAGVNSSHAQQTIRVGWTIPAEESKY
                     WMMRRPAEFPDLGKTYNVEWTQFQGTAPMTQALAAGALDCATQAPLSLANGVVGGNLK
                     AYIVAQHVFEKPGGFSVYWAVKDDSPIKTIADLKDKTVGISVIGGGTYGPFAMLLQKN
                     GVDPKKDIKLVEVGFAVSEDALRQGRVDAVNMNQPFAARAEAKGGTRKLFSLSEEMPN
                     IVHILEACRADFVDKNPDLVKAYVRDITSGMKKALANREETLKVVSEVLKAPVPVLDT
                     YLLKDNDFGRDPGAAPNFDAIQKMLDIYAATGMLPKMDVAQFKHPTIVAPLQ"
     gene            2644..4338
                     /gene="ilvD"
                     /locus_tag="VGA15_10395"
     CDS             2644..4338
                     /gene="ilvD"
                     /locus_tag="VGA15_10395"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9588139.1"
                     /translation="MKKWRSRVTTDGLDRAPHRAFMRAMGLDDEALAKPMIGVVSMKG
                     EQTPCNMTHDFQVDAAKAGIAEAGGTPREFTTISVSDGISMNHEGMKFSLLSRELIAD
                     SIEAVVHGLAYDALIGFGGCDKTLPGVMMGMIRCNVPSIFIYGGSALPGRFEGKTLTV
                     LDSYEAVGGFMTGEIDQATLAGIERACLPTIGACAGQFTANTMAMVSEAMGLTMPNVS
                     MVPGVYAERAQVARRAGRLVMQMLQQGGPLPREVVTRKALENGAAIVAATGGSTNAAL
                     HLPALANEAGISFTIDDVGRVFARTPLIGNLRPGGKYTAKDVYDIGGTAVVVRALVES
                     GHIDGSCLTITGRTIAEEYGEANLPDGEIITRTNAPIMPDGGVAVLKGNLCPDGAVIK
                     VAGLKKLVFEGTARVFEDEEECVDAVRNRKYAAGDVLVIRNEGPVGGPGMREMLGVTA
                     LIYGQGMGEKVALITDGRFSGATRGMCIGYVSPESFIGGPLALVREGDHIRIDAGARR
                     MDLLIDDNEFEKRHQAWKQRPPRHRAGALAKYARLVGQAPGGAVTHEGAADWPWFG"
     gene            complement(4366..5664)
                     /gene="kynU"
                     /locus_tag="VGA15_10400"
     CDS             complement(4366..5664)
                     /gene="kynU"
                     /locus_tag="VGA15_10400"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX9588140.1"
                     /translation="MSFGFDHEVLPMSSASMISAPMTSRDECVARDCVDPLRGFRDRF
                     MIPQGLIYLDGNSLGPMPRAAASVLNRTIEQEWGRDLIRSWNTAGWFDMPVRLGDRVG
                     TLIGAAPGQTLVCDTTSINLYKAIHAAIGLRPDRDVVIAEDESFPTDLYVVEGAMKSA
                     GRPMKRRLIGTDGPSIEALLDRKVAVAVLSHVNYRTGALLDIAVITKQLHDAGALVVW
                     DLCHSIGVVEIAFDLHAVDFAVGCTYKYLNGGPGSPAFISVATAHQATAQHPLSGWWG
                     HAAPFAFDRDFRPDAGIKRFLCGTQPIISLRGVDVALDAMEGVEVAALRQKSLALTEL
                     FMARVAALLPDLDVVTPRQPSLRGSQVGITFDKGYAVVQAMIERGVIGDFRAPDLMRF
                     GFAPLYVRFQDVWDAAEILAGCISAEVWRDPRFNQVLDVT"
     gene            5918..6715
                     /gene="kynA"
                     /locus_tag="VGA15_10405"
     CDS             5918..6715
                     /gene="kynA"
                     /locus_tag="VGA15_10405"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEX9588141.1"
                     /translation="MSQLKEISYSGYLQLDKILQAQLPLSAAHDEMLFIIQHQTSELW
                     LKLAIHELRATCRLIATDDLQPAFKTLSRVSRILEQLNSAWDVLRTMTPSEYTEFRGS
                     LGQSSGFQSWQYRMIEFLVGNKNAAMIHTHQHDQAVVGQLEAARLAPSIYDLTIQLLD
                     RRGLCIDRAIIERDVSVSHVESDSVVSAWLSVYQNPARFWELYELAEKLVDFEDYFRR
                     WRFNHVTTVERIIGFKRGTGGTSGVSYLRKMLDVVLFPELWRVRTQL"
     gene            6712..7374
                     /gene="kynB"
                     /locus_tag="VGA15_10410"
     CDS             6712..7374
                     /gene="kynB"
                     /locus_tag="VGA15_10410"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HEX9588142.1"
                     /translation="MTGLIDISPSLRVGMGVFPGDAVFSVSQTFTIGPNCPVNVASFA
                     MSTHCGAHVDAPLHYDSSGASVDMLDLSDFIGPARVIDARGNDPLCAFEEIASALDGA
                     PTRLLFKLMDRGDQLSWPTGFRTLAPETVERLALRGTRLIGVDVPSVDPETSKSLPSH
                     MVCRRHDIRIVENLVLADVVPGDYELIALPLKLRGLDAAPVRAVLRRSGPHEVDAGAG
                     NA"
     gene            7483..>8188
                     /locus_tag="VGA15_10415"
     CDS             7483..>8188
                     /locus_tag="VGA15_10415"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588143.1"
                     /translation="MKALPSLRGVQAESAPARGPASAMIEIDRVSQVFRTSGRQDHLA
                     LSEISLTIEDGAFVSILGPSGCGKSTLLYIVGGFVSPTKGVARMKGKAIAGPGPDRGP
                     VFQEFALFPWQSVLGNVMYGPRQQGVKRAEAEAQSRALIEMVGLKGFENFYPKELSGG
                     MKQRVALARTLAYHPAVLLMDEPFGALDAHTRTRLQNDLLNIWERDRKTVLFVTHSVD
                     EAVFLSDKVVVMTRSPG"
BASE COUNT         1627 a         2415 c         2526 g         1620 t
ORIGIN      
        1 gcgatgaccc ttctcggccc tcccgtagtt caggcatgag gtgtacatgc cctgacgaca
       61 tcgcttgcac tggccgcagc ccgcgtgaat ttccacgctg acgcgttcgc cgatttcgaa
      121 ttcgtcgacg ctgggtccga gcgctgcaac ggtgcccata tattcatggc cgggcgtgaa
      181 attcttgttg aagggcaggc cgccctgaat gctcgctggg gagccgccat ggatgatctg
      241 gagatcggtc gcgcagatcg caaccgcgtc gatgcgcacc aggacttctg cgcggcctgg
      301 aaccggtgtc ggtttgtcgc gcgggaaaag ctcgtccggg ttacccaaaa cccaggcttt
      361 catttgcgca ggcacgggaa actgtagcga tgtcttaaca gcggcaggtt cgtccattgc
      421 gatgggtcct tgtgagccga agaacctacc tgaaattgcc ggaagatgaa aatccccgtt
      481 atcggcccgg tcgcctcaac ccacttgtga ggccgacaac ttatagattt cgagcgcatc
      541 ggcatccgcg ccccgcgcgg atttcgccag cctgaacaat tgcgcggcca gtgccgacat
      601 cggcaccggg ctcgatgtat cgcgggcgac gtcgatcacc gtgtccagat ctttcagcat
      661 ggttgcgata tgcccgagcg gcggcgaatg aatgccctgc accatgcgtg gcacgaacaa
      721 ttgcagcgga atagaatcgg caaagccgcc ggcgagcgcc tccggcagcc gccgggcgtc
      781 gatcccggca tttaccgcga ggcgggtcgc ctccgcaagt accgccatcg cgcagccgac
      841 gatcacctga ttgcagagtt ttgaggtctg tccggcgccg gtcgggccca tatgggtaag
      901 cctgcgcgcc atcgctagaa catagggcct cgctctttcg atatcggcgg catcgccgcc
      961 ggccatcact gcgagtgtgc cttgttcggc gccgatggtg ccccccgaga ccggtgcatc
     1021 gatccacccc ataccgttgg ccaatttcag ccgcgccgcg atcgagcgcg ccgcgtccgg
     1081 gtggatcgat gagaagtcaa cgacgagctt tccggcgcca tccgccgtcg caaggccgtc
     1141 ggggccgaac accacgtcct ccaccgcggc ggcatcggtg aggcacatga agatgatgct
     1201 ggcgttggtg gcgacctcgc gcggaaagct tgcgagcctc gcgccggcct cgaccagtgc
     1261 ggcggccttg ccggaagacc ggttccagac cgtgacatca tggccagcat ccaccaggcg
     1321 ctgcgtcatc ggaaatccca tcatcccgag cccgacataa ccgagctttt ccgtgcttcc
     1381 ttggtccgtc ccgcgcgcaa tcgccattcc gtttctcctt cactgccgtc aagctctgtc
     1441 cggcatgctg gccttaccat acattccggc cttgctggtg gaatgcgcag tgcccaagcg
     1501 agccccgatg gcttgcctga ctgtttgaac catgaaaggc ttggcgcatg taattttggg
     1561 ctggcgcctg ttggcatcga aaaggtggag tggcatgatg cggataccat cgaaatggat
     1621 cgtagcggcc ggggctgcgg tcgccgttct ggcgggagtg aactcttctc acgcgcagca
     1681 gacgatccgt gtcggctgga ccattccggc cgaggaatcg aaatactgga tgatgcgcag
     1741 gcccgcggaa tttcccgatc tcggcaagac ctacaatgtc gaatggacgc aattccaggg
     1801 caccgcgccg atgacgcagg cgcttgccgc gggtgcactc gattgcgcga cgcaggcgcc
     1861 gctgtcgctg gcgaacggtg tggtcggagg caatctgaag gcctacatcg tggcgcagca
     1921 cgtcttcgaa aaacccggtg gcttttcggt ctattgggcg gtcaaggacg attcgccgat
     1981 caaaaccatc gccgatctca aggacaagac cgtgggcatc tcggtgatcg gcggcggcac
     2041 ttacgggcca ttcgcgatgc tgctgcagaa gaacggcgtg gacccgaaga aagacataaa
     2101 gcttgtggag gtcggcttcg cggtgtccga ggacgcgctg cgtcagggtc gcgtcgatgc
     2161 cgtaaacatg aaccaacctt tcgcggcgcg tgccgaggcc aagggcggca cccgcaaatt
     2221 gttttcgctg tccgaggaaa tgccgaacat cgttcacata ctggaagctt gtcgcgccga
     2281 ttttgtcgac aagaatccgg atctggtaaa agcctatgtg cgcgatatca cctccggtat
     2341 gaagaaagca ctcgccaatc gcgaagaaac actcaaggtc gtctctgagg ttctgaaggc
     2401 gccggtgccg gtgctggata cgtatctgct caaggacaat gacttcggca gggatccagg
     2461 cgctgcgccg aatttcgacg cgatccagaa gatgctcgac atttatgccg cgacagggat
     2521 gctgccgaag atggacgtcg cgcagttcaa gcacccgacg atcgtcgcgc cgctgcagta
     2581 gacaggccta tccacgggtc gtcccggcga gcgccgggac gacccggaga gagatttgtg
     2641 cacatgaaga agtggcgatc acgcgtgacg accgatggtc tggaccgtgc gccgcaccgc
     2701 gcatttatgc gcgcgatggg gctggacgac gaggccctcg ccaagccgat gatcggggtc
     2761 gtcagcatga agggcgagca gacgccctgc aacatgaccc acgatttcca ggtagacgcc
     2821 gccaaggcgg gaatcgcgga ggccggcggc acgccgcgcg aattcaccac catctcggtt
     2881 tccgacggca tcagcatgaa ccatgaggga atgaagttct cgctgctctc gcgcgagttg
     2941 attgccgatt caattgaagc tgtggtccac ggcctggcct atgacgcctt gatcggtttc
     3001 ggcggttgcg acaagacgct tcccggcgtg atgatgggaa tgatccgttg caacgtgcct
     3061 tcgatcttca tctatggtgg cagcgcgctg cctgggcgct tcgagggcaa aacccttacg
     3121 gtgctggatt catacgaggc cgtcgggggc ttcatgaccg gcgaaatcga tcaggcgacg
     3181 ttggccggca tcgagcgggc ctgcctaccg acaatcggtg cctgcgcagg acagtttacc
     3241 gccaatacga tggcgatggt ttccgaagcg atggggctga cgatgcccaa cgtgtcgatg
     3301 gttcccggcg tttatgccga gcgcgcgcaa gttgcccgcc gggccggacg tcttgtcatg
     3361 cagatgctgc aacagggcgg accgctgcct cgtgaagtcg taacgcgaaa agcgctggaa
     3421 aacggcgctg ccattgttgc cgcgaccggc ggatcgacca acgcggcgct gcatttgccg
     3481 gcacttgcca acgaagccgg catctccttt acgatcgacg atgtcggtag ggtgtttgcg
     3541 cgcacgccct tgatcggcaa tctccgtccg ggcgggaaat ataccgcgaa agacgtttac
     3601 gacatcggcg gcactgctgt cgtagtccgc gcgcttgtcg agagcggtca catcgacggc
     3661 agttgcctga cgattaccgg cagaacgatt gctgaagaat acggcgaggc gaatttgccc
     3721 gacggcgaga tcatcaccag aacaaacgcg ccgatcatgc cggatggtgg cgtggcggtg
     3781 ctcaagggca acctttgccc ggacggagcc gtcatcaagg ttgcgggcct caagaagctg
     3841 gtgttcgaag gcaccgcgcg cgtgttcgaa gatgaggagg aatgcgtcga tgccgtccgc
     3901 aatcgcaaat acgcagccgg cgacgttctc gtcatccgca atgagggccc ggtcggcggt
     3961 ccgggcatgc gcgagatgct gggcgtcacc gcgctgatct atgggcaggg catgggagag
     4021 aaggtcgcgc tgatcaccga cgggcgcttc tccggagcga cgcgaggaat gtgcatcggc
     4081 tatgtctcgc cagaatcgtt cattggcggc ccgctggcct tggtgcgtga aggcgaccac
     4141 atcaggatcg atgccggcgc gcgccgcatg gacctgttga ttgacgacaa tgaattcgaa
     4201 aagcggcatc aggcctggaa gcaacggccg ccgcggcatc gggccggggc gcttgccaaa
     4261 tacgcgcgcc tggtcggaca ggcccccggc ggcgccgtca cgcatgaggg tgccgcggat
     4321 tggccgtggt tcggttgacc cggaaaccac tattttgacg ccccctcagg taacgtcaag
     4381 aacctgatta aagcggggat cgcgccagac ctctgcgctg atgcacccgg caagtatctc
     4441 ggcggcatcc cagacatcct gaaatcgaac gtaaagtggc gcgaagccga agcgcatcag
     4501 gtctggcgcg cggaaatcgc cgatgacacc gcgttcgatc atcgcctgca cgaccgcata
     4561 acccttgtcg aaggttattc cgacctggct tccgcgcaac gatggctggc ggggtgtcac
     4621 gacatcaagg tccggcaaaa gcgcggcaac gcgcgccatg aaaagctccg tgagagcgag
     4681 actcttctgt cttagagccg ccacttcgac gccctccatt gcgtcgagcg ccacgtccac
     4741 accgcgcagg gagatgatcg gctgagttcc gcacagaaat cgtttgatgc ctgcgtcggg
     4801 ccggaagtca cgatcaaacg caaagggggc ggcatggccc caccaacccg ataacggatg
     4861 ttgcgcggtg gcctgatggg ctgttgcgac cgaaataaag gcgggagatc cgggacctcc
     4921 gttcagatat ttatatgtgc aaccgaccgc gaaatcgacc gcatgaagat cgaatgcgat
     4981 ttctaccacg ccgatggaat gacagagatc ccacaccacc agcgcaccgg cgtcgtgaag
     5041 ctgtttggtg atgacggcga tgtcgagcag tgcaccggtg cgataattca cgtgcgagag
     5101 cacggcgacc gcgacctttc ggtcaagcag ggcctctatg gacgggccat ccgtgccgat
     5161 caatcgccgc ttcatcggac gacccgcgga cttcattgcg ccttcgacga catagaggtc
     5221 tgtcggaaac gactcgtcct cggcaatgac gacgtcgcga tcaggcctca agccgatcgc
     5281 ggcatggatc gccttgtaga gattgatcga tgtggtgtcg caaaccagcg tttggcccgg
     5341 agccgccccg atcagagttc cgacgcggtc gccgagccgg actggcatgt cgaaccatcc
     5401 ggctgtattc caactcctga tgaggtcacg gccccactct tgttcgatcg tacggttcaa
     5461 gacgcttgcc gcggcgcgcg gcatcggacc gagcgaattg ccgtcgagat agatgaggcc
     5521 ttgaggaatc ataaaacggt cgcggaagcc gcggagcgga tcaacgcaat cgcgcgcaac
     5581 gcactcgtcg cgcgacgtca tgggggctga tatcatagag gctgacgaca tcgggagaac
     5641 ctcgtgatca aaaccaaacg acatgtcgga tcatggggca cgagatttaa aatccaggtt
     5701 ctcgaataaa ccaagcatgc ctgttcccaa aacaacggca actgcttgtt tgcggccgcg
     5761 cgcgggcgtt cttgctcaga atccatgaaa tcccaattga cagcaaaaat ctatcctttt
     5821 aagcttaaaa tatcgtcgct tcgctttcgc gaagctaagg ttgagccggt atggcgcgcc
     5881 cgaaaatatc atcgactcgt cgtaggacgc ggacttgatg agtcaactca aagaaatatc
     5941 ctattccggc tatctccagc tcgacaagat tctgcaggcg caactgccgc tttccgccgc
     6001 ccatgacgag atgttgttta ttatccagca ccagacctcg gagctctggc tgaagcttgc
     6061 aattcacgag ctgagagcca cctgccggtt gattgcaaca gacgatctgc agccggcttt
     6121 caagacattg tcgcgcgtgt ctcgcatcct tgagcaactc aactcagcct gggatgtcct
     6181 tcgcacgatg acgcccagcg agtacaccga gtttcgtgga tcgctcggcc agtcgtccgg
     6241 ctttcagtcc tggcaatatc gcatgatcga atttcttgtc ggcaacaaga atgctgccat
     6301 gatccacact caccagcatg accaggccgt cgtcggacaa ctcgaagcgg cccgtctggc
     6361 cccgagtatc tatgatctca cgatacagct gctggatcgc cgcggcctct gtatcgatag
     6421 ggccatcatc gagcgcgatg tctcggtatc tcatgtcgaa agtgacagcg tcgtgagtgc
     6481 gtggttgtcg gtctatcaaa accctgcgcg attctgggaa ttgtacgagc ttgccgaaaa
     6541 gctggtggat ttcgaagatt atttccgccg ctggcgtttc aatcacgtta cgacagtcga
     6601 gcggatcatc ggattcaagc ggggcaccgg cggcacttcg ggagtttctt acctgcgcaa
     6661 gatgctggat gtggtgttat ttcccgagct ctggcgggtc aggacccagt tatgacgggc
     6721 ctgatcgaca tctcgccttc gcttcgtgtc ggcatggggg tgtttcccgg cgacgctgtt
     6781 ttttcagtca gccagacatt tacaattggg cccaactgtc ccgtcaatgt agccagcttc
     6841 gcgatgtcaa cgcactgcgg cgcccatgtc gatgcacctc tgcactacga ttcatcgggt
     6901 gccagtgtcg atatgctgga tctcagcgat ttcattggcc ccgcaagggt gattgatgct
     6961 cgtggaaatg acccgctttg tgcttttgag gaaatcgcat ccgccctcga cggcgctccc
     7021 acgcgactgc ttttcaagtt gatggacagg ggcgatcaat tgagctggcc gacaggtttc
     7081 cggacgctgg ctccggaaac cgtggagcgc ctggctcttc gtggcacacg gctaattggt
     7141 gtggacgttc cgtctgtcga tccggagacg tcgaagagcc tcccgtcgca catggtgtgc
     7201 cggcggcacg atattcggat tgtcgagaat cttgtgctgg ccgacgtagt acccggcgac
     7261 tacgagttga tcgcgctgcc gttgaagctc aggggccttg acgcagcgcc ggtgcgcgcg
     7321 gtccttaggc gatctggccc ccatgaagtc gacgccggcg ctggtaatgc ctaaatcgac
     7381 atcgccaacc cttgcacgtt tccgctatgc tcggaccgtt agcgtccgaa aaccgagaag
     7441 gcagctgtgc ctgacggatc ctcgtccgga agagaagcgg gaatgaaggc attgccatcg
     7501 ctgagaggtg ttcaggcgga atccgcgccg gcacgcgggc cagcgagtgc catgatcgag
     7561 atcgaccgtg tctcgcaggt gtttcgcact tccggccgcc aagaccattt ggcgttgtcg
     7621 gagatttcgc tgacaatcga agatggcgcc ttcgtctcca ttctcggtcc ctccggctgc
     7681 ggaaaatcga ccttgctcta tatcgtcggc ggttttgtca gtccgaccaa aggcgtggcc
     7741 cggatgaagg gcaaggcgat cgccgggccc ggtcccgatc gcgggccggt gtttcaggag
     7801 ttcgcgctgt ttccctggca gagcgtgctc ggcaatgtga tgtacggccc tcgccagcag
     7861 ggcgtgaagc gcgcggaggc cgaagcgcaa agccgtgcgc tgattgagat ggtcggcctc
     7921 aagggctttg agaattttta tcccaaggaa ttgtccggcg gtatgaagca gcgcgtcgcg
     7981 ttggcgcgca cgctggctta tcaccccgcc gtgctgctga tggacgagcc gttcggcgcg
     8041 ctggatgcac atacccgcac acggttgcag aacgacctgc tcaatatctg ggaacgggat
     8101 cgcaagacgg tgctgttcgt tacccattcg gtcgacgaag cggtgtttct gtcggacaag
     8161 gtcgtggtga tgacgcgttc gcccggcc
//
LOCUS       DASWGM010000358         7563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2255616,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000358 DASWGM010000000
VERSION     DASWGM010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2255616"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..644
                     /locus_tag="VGA15_10420"
                     /pseudo
     CDS             423..644
                     /locus_tag="VGA15_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            1497..2723
                     /locus_tag="VGA15_10425"
     CDS             1497..2723
                     /locus_tag="VGA15_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX9588144.1"
                     /translation="MTVEWFKPRGYVHFDAPVGNAFAQKVLDRVFVTKHSWLPLIYYI
                     KRTKRYKASKGKTVFKDRPIMYASHRDACILSKYAWELTTALDAHYEALGLEQHVIAY
                     RKLGRANYDFSADAFNFARAKPVSVVLCYNITGFFDNLDHRILKERLKRVLRVGELAQ
                     DWFAVFRHVTQYKAIERDELAAHSRFGPRLSMKLREPLATISELLAERIQIKRNPKRV
                     GIPQGTPISSVFSNLYMIDIDQAMVGLCKKRGALYQRYSDDILVVCNPEDEASINSTM
                     EALIKSHLLEIKPEKTETIVFDKLAPHAFQYLGFDISPDGAVIRAGSLGRRWRKARRS
                     IARIKKIGLIAVAEGKATKIYTKKLRRRFLPVGVRNFSSYARRAAKAFGNKKIVRQVL
                     RLERMIDTALLDLDPH"
     gene            3087..4232
                     /locus_tag="VGA15_10430"
     CDS             3087..4232
                     /locus_tag="VGA15_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX9588145.1"
                     /translation="MIPTQRLPASLTPLDLALAALLDRLEPVAPIKLPLAQALGCVAA
                     EMPPLRAYPPCDIAAADGFAFCARDLVGASSYSPLPLAKSLAWVEAGDAMPQGCDCVI
                     DADLVDQTGPMAEVLGEAIPGQGVRRAGGDIAEGSLVLASGRRVRPLDLGIARAAGLD
                     HLKVRRPRLRLLNIPATSGTTATAQLISEYARGAGADMACAEATGRDAASIAKALDTA
                     IDAAIEPGSCDLLVTIGGSGVGRTDAAVVALAQRGEVIAHGIALQPGRTAAVGRIGKV
                     PVIALPGAPDQALAAWWTLALPVLDRLSARQSRQTLTLPLARKIASSVGIAEIVLLER
                     NDGTWMPLAVGELSLETIARADAYLAVASGREGFAAGTPVDAYMMSD"
     gene            4248..6200
                     /locus_tag="VGA15_10435"
     CDS             4248..6200
                     /locus_tag="VGA15_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HEX9588146.1"
                     /translation="MSNANQPKSRDSVEQDQFLTILSREDALARFEAALFPRALPCET
                     RGLADALGCALAEDIVAPMDVPPFDRSNVDGFAVRSADLAAAGEGKPVCVMLNDEVIA
                     CGTVPVRPVLSGTATSIATGGPVPRGADAVIMVEHTQPVGHRAIEIRRAASPGQFISY
                     AGSDIARGEALLRAGTMIGSREIGMLAACGIAHVSVARRPRVAVISTGDELVQPGQPL
                     RAASIYDTNGAIVTAAVAENGGEAVFLGAVPDDEEELEAEMREALGTCDMLVLSGGTS
                     KGAGDVSHRIIARLGQPGIIAHGVALKPGKPLCLAVCDGKPVVILPGFPTSAMFIFHD
                     MIVPVLRRMAGLPPRSVAKVTASIPVRIASELGRTEFVMVSLVQGTDGLIAYPSDKGS
                     GAITSFAQADGFVRIEALADQMPAGTKAEVTLFTPHVRVPDLLIVGSHCTGLDLVTAP
                     LARAGLLVRSIAVGSLGGLAAAKRGECDLAPIHLFDEMTESYNAPFLADGLELVPGWR
                     RLQGVVFRLGDARFTGLTTAQDAVRAALADPTCVMVNRNQGAGTRVLIGRLLGNARPD
                     GYWNQPRSHNAVAAAVAQHRADWGMTIAPVADAAGLGFIPLAEEHYDFALVTARKQRP
                     AVQAFLEALASEQSRAALTQAGFRPA"
     gene            complement(6398..7030)
                     /gene="mobA"
                     /locus_tag="VGA15_10440"
     CDS             complement(6398..7030)
                     /gene="mobA"
                     /locus_tag="VGA15_10440"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEX9588147.1"
                     /translation="MTSEAIPRTPGVLLAGGLARRMGGGDKPMRQIGGRTILERVIAR
                     LKPQCDGLILNANGDPARFAAFGLPVIADTVENFPGPLAGILAALDWAAANRPEVSWV
                     LSAAADCPFLPRDLVARLHRARAEQNAELAVAASDGQSHPVIGLWSVALRETLRHALV
                     VEDIRKIDRWTARFRLATVTWPVDELDPFFNANTMDDIATAERLALLDGG"
     gene            complement(7020..>7563)
                     /locus_tag="VGA15_10445"
     CDS             complement(7020..>7563)
                     /locus_tag="VGA15_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX9588148.1"
                     /translation="SGCAQGTAFGDLLEAVESVALPTAELRTSWLYQMTHAINTMPSL
                     YLEAGAIHGCVLCKEGTPVCYTEDVGRHNAVDKIAGWIYRHGVDPADKILYTTGRLTS
                     EMIIKTVRMGIPILVSRSGFTAWGVDLARQVGLTLVGRARGKRFIALSGQERIIYDQN
                     LAFVEEESARHKRKGGDRDE"
BASE COUNT         1359 a         2358 c         2397 g         1449 t
ORIGIN      
        1 ttgacgccag cgtcggggcg tcaggaccac acgattttgc cgtccgcgac aacatctctc
       61 gtccgcgtgc ttgtgatcgc tcacaggctc tttaagaacc cgccctgcca tcacgccgca
      121 cgctcaacgc tgccgcgtcc atcgcatccc gacccaacgt ccgtgacgat catgatacgc
      181 ccctctctcg ggccgggatg ggaaaagttc tagagatgat ttggggtgag tggaaagcga
      241 aatatttttg caaatgggac tcgacacgcc tgtcaacaaa ccgcccgacg ggcaaatcac
      301 ttgagccggt gttgttgtca atccgtagga tgggttgagc tcttcgcgaa acccatcacc
      361 tccacgaaat gcaattgatg ggtatcgctg agtttatcat cgggcgcgct gatctcgttc
      421 aagaacgcgg cgagctcgaa atcgtcgtcg agggcgacaa gcagctgttc aatcaatatg
      481 gcgtcatgct ggtgaatccc gaaaaatatc cgtccgtgaa gaagcaactc ggccaggcat
      541 tcgtcgactg gctggtctca gtggaatgcc aggccgcgat cgccggctac aagatcgatg
      601 gccagcagct gttctttccc aatgcggaga agaaggggac ttgagcaacg ctttcgttcc
      661 gcgcgccgag ctaaccattt ttttcgacca cctatccgcg tcgtcccggc gaacgccggg
      721 atccatacgc cgtgcattct cggcttgggc actgaagtag aaacctgtgc gtctccccaa
      781 cgtcaggggt tatgggtccc ggccttcgcc gggacgacgc agcgcggggc gcgttcgcac
      841 aaagaccgtc atactccgcc accgggtctc gccttcggcg agcccgatga caggctccat
      901 cggagtatcc agtacgccgc agttttgcgc ttccatcact ggcgcctcgg aatactgatc
      961 gcccgccgga acctgtcgtt gttggctgcg gacaggcgcc cggtacagac gggtggaacc
     1021 gacagcgcac ccatttgcta ttgaaaagat tgcctttttc gcccgcgagg ttagagattt
     1081 tctggtattt gccgccacca tttaattcac aactgagagt tctcaccgtt cctcgaatcg
     1141 cttatgggta gctaggaagt cgcgataggt tccttggggg gaacttgttt cgctttccta
     1201 tctcttgccg ccggaattgg ccggacggcg cggacctgtc aacttgttgc gcgaatacca
     1261 tgacgagact gatcaacagt atcggccagg gatgatcctg cagcgatgag acagcgaaca
     1321 tagttcgaac ctccacgatc aagttatacc gccttgcgtt ttgagacccg cgttagacgt
     1381 gcccatttga gacaatgggg atgactatcg gattatggcg tgcgtttggg gggtgttcgg
     1441 ttacggctga ggctcagggt cagagctaac cccccaaact ctgcaatggg tggcgcttga
     1501 ccgtcgaatg gttcaaacct cggggatatg tgcatttcga cgccccggtc ggaaacgctt
     1561 ttgcgcaaaa agtcttggac cgcgtcttcg tcaccaagca ttcatggctt ccacttattt
     1621 actacatcaa gcgcacaaag cggtacaaag ccagcaaagg gaaaaccgtc tttaaagacc
     1681 gacccatcat gtacgcttca catcgcgatg cgtgcattct ctcaaaatat gcttgggagc
     1741 tgacgaccgc gctcgatgct cactatgaag cgctcggctt ggagcagcat gttatcgctt
     1801 atcgtaagct gggacgagct aactatgatt tctcggcaga cgcatttaat ttcgcgcgtg
     1861 cgaagcccgt cagtgttgtt ctttgctaca acataacggg cttttttgac aaccttgacc
     1921 acagaattct caaagagcgc ctgaaacgag ttcttcgagt gggtgaactg gcgcaggact
     1981 ggttcgctgt gtttcgccat gtcacgcaat acaaagcgat tgagcgagat gagctggcgg
     2041 cgcattcaag gtttggcccg cggctgtcga tgaaattgcg agagccactc gcgaccattt
     2101 cggagcttct cgcagagcgc atccagatca agcgcaatcc taagcgcgtc ggtatcccac
     2161 aaggcacgcc aattagcagc gtattctcga acctgtacat gattgacatt gatcaggcga
     2221 tggttgggct gtgtaagaag cggggtgcgc tttatcaacg ctattctgac gacatcctgg
     2281 tcgtgtgtaa tcccgaggat gaagcctcaa tcaattcaac catggaggcg ctcatcaaga
     2341 gccatctcct agaaatcaag ccggagaaga ccgaaacgat cgttttcgat aagctggccc
     2401 cacacgcttt ccaatatctc ggttttgaca tatcgccgga tggtgccgtg atcagagccg
     2461 gttcactggg caggcgatgg cgaaaggcga ggcgaagtat cgcacgcata aagaaaatcg
     2521 gcctaattgc agtggcagag ggcaaggcaa ccaaaatcta cacgaagaag ttgcggcgac
     2581 gatttttgcc ggtcggagtg agaaactttt cctcttacgc gcggcgcgcc gccaaggcgt
     2641 ttggcaataa aaagattgtt cgacaggttc tgcgtctgga acgaatgatc gatacggcgt
     2701 tgcttgacct agacccacat tgaataaaga aagcgcccgc tggcccggct cacgctgcgc
     2761 gaaggcggac aggcgcggcg ggcgccgatg tgatgcaggc ggcaagaact taactctcgt
     2821 cgtccctgtg cacgcaggga cccataaccc ctggccttca aaagtgaaaa aaggcctctg
     2881 ctccaatgcc caaccgagaa tccgcgccgt atgggtcccg gcattcgccg ggacgacgaa
     2941 agtttgttaa acctctccta tgccagcatg tatcgtgtcg ccgccttttc ccaatcttca
     3001 ccggcacggc ggttggtgcc cggcgttcgc cgggacgacg ccagcaacgg ttgccaaggt
     3061 agcgacgcct ggtccaccat cgcctgatga tcccaaccca gcgcctgcct gcttcgctga
     3121 cgccgctcga cctggcgctc gccgcgttgc tggatcggct cgaaccggtg gctccgataa
     3181 aacttccgct ggcgcaggcg ctgggctgcg tcgctgccga gatgccgccg ctgagggcgt
     3241 atccgccgtg cgacatcgcc gcggccgatg gctttgcgtt ttgcgcccgc gatctcgtcg
     3301 gcgcgtcgtc ctattcgccg ctgccgctgg cgaaatcgct cgcttgggtc gaagccggcg
     3361 acgccatgcc gcaaggttgc gactgcgtga tcgatgccga tctggtcgat cagaccggcc
     3421 cgatggcgga ggtgctgggg gaggcgatac cggggcaggg cgttcgccgc gccggtggcg
     3481 acatcgccga aggcagtctt gttctcgcat cggggcggcg cgttcgcccg ctcgaccttg
     3541 gaatcgcgcg cgcggcggga ttggatcatc tgaaggtgcg ccgcccgcgc ctccgcctcc
     3601 tcaacattcc cgcgacctca ggcaccaccg cgacggccca gctgatttcg gaatacgcac
     3661 gcggggccgg tgcggacatg gcctgcgccg aggccacggg gcgcgatgcg gcgtcgatcg
     3721 caaaggcact tgataccgcg attgatgccg cgattgaacc cggctcgtgc gatttgctcg
     3781 tgaccatcgg cggcagcggc gtcggccgca ccgatgccgc ggtggtggcg ttggcccagc
     3841 gcggcgaggt catcgcgcat ggcatcgccc tgcagcccgg ccgcaccgct gccgtcggca
     3901 ggatcgggaa agttcccgtc atcgccttgc ctggcgcgcc ggaccaggcg ctcgccgcgt
     3961 ggtggacgct ggccctcccg gtgctggacc gtttgtccgc ccggcagtcg cggcaaacgt
     4021 taacgctgcc gctggcgcgc aagattgcct cgagcgtcgg catcgccgag atcgttctgc
     4081 tggaaagaaa tgatggaacc tggatgccgc tcgcggtcgg cgagctctcg cttgaaacca
     4141 tcgcccgcgc cgatgcgtat ctggccgtgg cgagcggccg cgaaggattt gccgcgggca
     4201 cgccggtgga tgcttatatg atgtcggact gatattatga gttccggatg agcaatgcga
     4261 atcagccgaa aagccgcgac agcgtggagc aggaccagtt cctgaccatc ctgtcgcgcg
     4321 aagatgcgct ggcgcgcttc gaggccgcgt tgtttccgcg cgcccttcct tgcgaaacgc
     4381 gcgggctggc cgacgcgctg ggctgcgcgt tggccgaaga tatcgtggcg ccgatggatg
     4441 tgccgccgtt cgatcgctcc aatgtcgacg gctttgcggt gcgatccgcc gatctggcgg
     4501 cggccggcga aggcaagccg gtctgcgtca tgctgaacga cgaggtgatc gcctgcggca
     4561 ccgtgccggt gcggccggtg ctgtcgggca cggccacgtc gattgcgacc ggcggtccgg
     4621 ttccgcgcgg cgccgatgcc gtcatcatgg tcgagcacac ccaaccggtt ggccaccgcg
     4681 cgatcgagat caggcgggcc gcctcgccgg gacaattcat ctcctatgcc ggctccgaca
     4741 tcgcgcgcgg ggaggcgctg ttgcgcgccg gcaccatgat cggctcgcgc gagatcggca
     4801 tgctcgccgc ctgcggcatc gcgcacgttt ccgtggcgcg tcgcccgcgg gtggccgtga
     4861 tctccaccgg cgacgaactg gttcagcccg gccagccgct gcgcgcagct tcgatctacg
     4921 acaccaacgg tgcgatcgtc acggcggcgg tggctgagaa tggcggcgag gcggtgtttc
     4981 tcggcgcggt gcccgatgac gaagaagagc tcgaagctga gatgcgcgag gcgctaggga
     5041 cgtgcgacat gctggtgctt tccggcggca cctcgaaggg cgcgggcgat gtgtcgcacc
     5101 gcatcatcgc gcggctcggc caacccggca tcatcgcgca cggcgtcgcg ctgaagccgg
     5161 gcaagccgct gtgtctcgcg gtctgcgacg gcaagccggt ggtgatcctg ccgggatttc
     5221 cgacctccgc gatgttcatc ttccacgaca tgatcgtgcc ggtgctgcgg cgcatggcgg
     5281 gcctgccgcc gcgttccgtt gccaaggtca ccgcaagcat cccggtgcgg atcgcttctg
     5341 aactgggccg caccgaattc gtgatggtgt cgctggtcca aggcacagat ggcttgatcg
     5401 cctatccctc cgacaagggt tctggtgcga tcacctcgtt cgcgcaggcc gacggttttg
     5461 tgcggatcga ggcgctcgcc gatcagatgc cggccgggac caaggccgag gtgacgctgt
     5521 tcacgccgca tgtgcgggtg ccggatctcc tgattgtcgg cagtcattgc acgggcctcg
     5581 atctcgtcac cgcgccgctg gcgcgcgcgg gcctgctggt gcgttcgatc gcggtcggca
     5641 gcctcggcgg attggcggcg gcaaagcgcg gcgaatgcga tctggcgccg atccatctgt
     5701 ttgacgagat gaccgagagc tacaacgcgc cgtttctcgc cgacggactt gaactggtgc
     5761 cgggttggcg gcggctgcag ggcgtcgtgt ttcgtttagg cgatgcgcgt tttacgggat
     5821 tgaccaccgc gcaggacgcg gtgcgcgcgg cgctcgccga ccccacctgc gtcatggtca
     5881 accgcaatca gggggcgggc acgcgggtcc tgatcggccg gctgctcgga aatgcgcgtc
     5941 ccgacggcta ctggaatcag ccgcgttcgc ataacgcggt ggccgcggcc gtggcgcagc
     6001 atcgcgccga ttggggcatg accatcgccc cggtcgctga cgccgccggc ctcggcttca
     6061 ttccgctggc cgaagagcat tatgatttcg cgctggtgac ggcgcgaaaa cagcgccccg
     6121 ccgtgcaggc gtttctcgag gcgttggcgt cggagcaaag ccgcgcggcg ctgacgcagg
     6181 cggggtttcg gccggcttaa gttggcatcg tgtcccggac gcggtgcagc gttctccacg
     6241 ctgctccgca gagccgggac ccatcgtaac gcatgtgggc cccggatcag cagcgcacca
     6301 cgccgcaaga gcggcgcgct gcgcagcatc cggggcacgc ccggctcacg ctcgcaaggg
     6361 ctcgcttggc cgggacgacg ccgagaaaca aattagctca acccccgtcc agcagcgcca
     6421 gacgctcggc ggtggcgatg tcgtccatgg tgttggcgtt gaagaacggg tcgagttcat
     6481 ccaccggcca ggtcacggtg gcgagccgaa agcgcgcggt ccagcgatcg atcttgcgga
     6541 tatcttccac caccagcgcg tggcgcaacg tctcgcgcaa cgcgacgctc cataatccga
     6601 tcaccggatg cgactggccg tcggaggccg cgaccgcaag ctctgcgttc tgttcagccc
     6661 gcgcccgatg caggcgtgcg acgagatcgc gcggcagaaa cgggcagtcc gccgcggcac
     6721 tgagcaccca cgaaacttcg ggccggttgg ccgcggccca gtccagcgcg gccaaaatgc
     6781 cggcgagcgg ccctggaaaa ttctcgaccg tgtcggcaat gacgggcagg ccgaacgccg
     6841 caaagcgggc cggatcgcca ttggcattga ggatcagccc gtcgcattgc ggttttaggc
     6901 gcgcgatgac gcgctccagg atggtgcggc cgccgatctg gcgcatcggc ttgtcgccgc
     6961 cgcccatccg ccgcgccagc ccgccggcca gcagcacgcc gggtgttcgc ggtatcgctt
     7021 cactcgtcac ggtcgccgcc cttgcgcttg tgacgcgcgg attcctcttc gacgaacgcc
     7081 agattctgat cgtagatgat gcgctcctgg cccgacagcg cgatgaatcg ctttccccgc
     7141 gcgcgcccga ccagggtcaa cccgacctgc cgcgccaggt cgacgcccca cgccgtaaag
     7201 cccgaacgcg agaccagaat gggaattccc atccgcaccg tcttgatgat catctccgag
     7261 gtcagtcttc ccgtggtgta gagaatcttg tcggcgggat cgacaccgtg gcgatagatc
     7321 cagcccgcga tcttgtcgac ggcgttgtgg cggccgacat cttcggtgta gcacacaggc
     7381 gtgccttcct tgcacagcac gcagccgtgg attgcgcctg cctcgagata gagcgacggc
     7441 atggtgttga tggcgtgcgt catctgatag agccaggacg tgcgcagctc cgctgtgggt
     7501 agcgcgacgc tttccacggc ctccagcaga tcgccgaacg ccgtgccctg cgcgcagccc
     7561 gaa
//
LOCUS       DASWGM010000359         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_39128,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000359 DASWGM010000000
VERSION     DASWGM010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39128"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VGA15_10450"
     CDS             <1..821
                     /locus_tag="VGA15_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588149.1"
                     /translation="EFPEIMVLGPGTIGDTTFASELVAASAPGGVDAVSYHHYGALSE
                     RCSGSAALEAALSEAWLSRTDQTLSFYRLLRDRFEPGKPIWLTETAEAACGGNRWAAT
                     FLDTFRYLDQLGRLAKAGVQVVMHNTLAASDYGLLDERTLAARPNYWGALLWRQLMGT
                     TVLDPGVPIQAGFHVYAHCQREAPGGVTLLVINTGRDTARSLTLPNASERFTLDAASL
                     QDGIVRLNGQPLQLDATDDLPGIAGVRTAAGTLRFAPATITFLSIPATGNSACR"
     gene            939..1856
                     /locus_tag="VGA15_10455"
     CDS             939..1856
                     /locus_tag="VGA15_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX9588150.1"
                     /translation="MYRQIALLCSIVVALSAASVARAAESEPCRDVRLRFEAIKPQIS
                     AIEVSLTLFSAVDKNCLDLATELLDHGASVDARDRLGARPLSHAARFGHLGMVDLLLA
                     HGAPIDARNLAGATALYFAAEGGHTPVAQRLIERGADVKLAGRSRISPVAAAAYAGND
                     AIVEALLAAGADPQAPDETGKPPIVYAAAGAQLDIVKRLLAQNIDINARYPNDLTLLM
                     WASGPDEKAPEAQAVKVVSYLLDAGARIDDRDDRGRTALMIAAEGGHAEIANLLTGRG
                     ADASLKDKAGKRAADLTVLSSLRERLTRP"
     gene            complement(1863..2450)
                     /locus_tag="VGA15_10460"
     CDS             complement(1863..2450)
                     /locus_tag="VGA15_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9588151.1"
                     /translation="MRGKLNLGTSGVVALAAVLSLCTTPLHAQSIEEKTAVCAGCHGA
                     DGKPVDKTIPNIWGQQAGYIYIELRDFKRGDRKSDIMQPIVSSMQRDDMQAIAEYFSK
                     KPWLDLGQPRASKEITYRASNANRSVGCTGCHLDHFQGDGTVPRLAGLGRKYLTKTMA
                     DFRTRARGNNPGMSDLMLATSPDDLAALAEYLSGL"
BASE COUNT          443 a          772 c          783 g          476 t
ORIGIN      
        1 aagaatttcc ggagatcatg gtcctcggtc ccggcacaat cggtgacaca accttcgctt
       61 cggagctggt cgccgcatcc gcccccggcg gcgtggacgc ggtttcctat catcactatg
      121 gagcgctttc ggagcggtgc agcggctcgg ccgcgctgga ggccgcgctt tccgaagcgt
      181 ggctctcccg gaccgatcag accctgagtt tctaccggtt gcttcgggac cgattcgaac
      241 ccggcaagcc gatctggctg accgaaaccg cggaggcagc atgcggcggc aaccgctggg
      301 ccgcaacctt tctggatacg ttcaggtatc tcgaccagct tgggcgactg gcaaaggccg
      361 gggttcaggt cgtgatgcac aacaccctgg cggcgagcga ttacggcctg ctcgacgaga
      421 gaacgctggc agcccgacca aattactggg gcgcgctgct gtggcggcaa ttgatgggga
      481 ccaccgtgct tgaccccggt gtgccgatac aagcgggctt tcatgtctat gcgcattgcc
      541 agcgcgaagc cccgggcggt gttacgcttt tggtgatcaa cacgggccgc gacaccgcgc
      601 gttcgctgac gcttccaaac gcctcggagc gttttaccct ggatgcggcg agcttgcagg
      661 acggaattgt gcgtctaaac ggccaaccat tgcaactgga cgcgacggat gatctgccgg
      721 gcatcgcagg tgttcggaca gccgccggca cactgaggtt tgcgccggcg acgattactt
      781 ttctgtccat tcccgcaacc ggaaacagcg cctgtcgata aggaaccatc aagtggatgt
      841 tgagcggacg gataccgaga caaccgcctg ttcgcactct tggaattgcg cagccgtcat
      901 gccaccctca cccgacatgc ctgactggga gagaccgcgt gtaccgtcaa atcgctttgc
      961 tatgttcgat tgttgtcgcg ttgagtgcag cctcggttgc gcgcgccgcg gaatccgagc
     1021 cttgccggga cgtccggctt cggtttgaag cgatcaagcc gcagatcagc gcgatcgagg
     1081 tgtcgttgac gctgttttcc gccgtcgaca agaattgtct cgatctcgca accgaactgc
     1141 tcgaccacgg cgcatcggtc gacgcgcgcg accggcttgg cgcccggccc ctcagtcacg
     1201 ccgcgagatt cggtcatctc gggatggttg accttcttct ggcgcacggc gctccgatcg
     1261 atgcccgcaa tctcgccggc gccaccgcgc tgtattttgc cgccgaaggc ggccacacgc
     1321 cggtcgcgca gcggctgatc gagcgcggcg ccgacgtcaa actcgccggg cgcagcagga
     1381 tctctccggt cgcggcggcc gcctatgccg gcaacgatgc gatcgtcgaa gcgctgctcg
     1441 ctgctggagc tgacccgcaa gcgcccgacg aaaccggcaa gccgccgatc gtctatgcgg
     1501 cggccggcgc acagctcgac atcgtgaagc ggttgctggc gcaaaatatc gatatcaacg
     1561 cccgctatcc caacgatctc accttgctga tgtgggcatc gggcccggat gaaaaagccc
     1621 cggaagcgca ggcggtcaag gttgtttcat atctgctgga cgcaggcgcc cgtatcgatg
     1681 atcgggacga ccgcggccgc accgcgctga tgattgcggc cgaaggcggc catgcggaga
     1741 tcgccaatct attgacgggg cgaggagcgg atgcgtcgct caaggataag gccggaaaac
     1801 gcgccgcaga tctcaccgtg ctgtcttcgc tgcgcgaacg gctgacgcgg ccctgaaccc
     1861 cttcagagac ccgacagata ttcggccagc gcggcgaggt cgtccggaga ggtggcaagc
     1921 atcaagtcgg acatgccggg attgttgccg cgggcccggg tgcggaagtc ggccattgtt
     1981 tttgtcaggt acttcctgcc caggcccgcc aggcgaggca ccgtgccgtc gccctgaaaa
     2041 tggtcgagat ggcagcccgt gcagcccacc gagcggttgg cgttcgaggc ccggtacgtg
     2101 atttcctttg acgcacgagg ctggcccagg tcaagccagg gcttcttcga gaaatattcg
     2161 gcgatggcct gcatgtcgtc gcgctgcatc gatgaaacga tcggctgcat gatgtcgctc
     2221 ttgcggtcac cccgtttgaa atcgcgtagt tcgatataga tgtagccggc ttgttgcccc
     2281 caaatgtttg gtatcgtctt gtcgaccggt ttgccgtccg cgccgtggca gccggcgcac
     2341 accgccgtct tttcttcaat gctttgagcg tggagcgggg ttgtgcagag gctcaataca
     2401 gccgccagtg cgacgacgcc ggaagtgccc aaattcaact tgccgcgcat ctatccgatc
     2461 cagtggagac taat
//
LOCUS       DASWGM010000360         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2532698,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000360 DASWGM010000000
VERSION     DASWGM010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532698"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /gene="hemA"
                     /locus_tag="VGA15_10465"
     CDS             <1..921
                     /gene="hemA"
                     /locus_tag="VGA15_10465"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX9588152.1"
                     /translation="RKQAALVFTSGYVSNQTGIATIAKLIPECLILSDELNHNSMIEG
                     VRQSGCEKQIWRHNDVEHLERLLQAAGQHRPKLIVFETVYSMDGDVAPMHCICDLAER
                     YGAMTYADEVHAVGMYGARGAGVAERDGVLHRIDVLEGTLAKAFGCMGGYIAGNSALI
                     DAVRSYAPGFIFTTALPPPICAAATAAIRHLKASNWERQRHQERAARVKAVLNAAALP
                     VMPSDTHIVPVLVGDPERCKAASDLLLTEHGIYIQPINYPTVPRGLERLRITPSPYHD
                     DALVDALAEALLDVWARLGLAPKRWATAAE"
     gene            1096..2571
                     /locus_tag="VGA15_10470"
     CDS             1096..2571
                     /locus_tag="VGA15_10470"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX9588153.1"
                     /translation="MNDQSKRPPLTHASGTPVTDNLNIQTAGPRGPALLQDVWLLEKL
                     AHFHREVIPERRMHAKGSGAHGTFTVTHDITRYTKAKIFSQIGKQTPMFARFSTVAGE
                     RGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFFRDPLRFPDLNHAIKRDPRTGMR
                     SADNNWDFWTLLPEALHQVTIVMSERGIPRSFRHMHGFGSHTYSFINAANERVWVKFH
                     FRTQQGIQNLTDAEAEARVGKDRETHQRDLFENIEAGNFPRWTLFIQVMTDAQAKAFP
                     YNPFDLTKVWPKGDFPLIEVGYFELNRNPENVFAEVEQAAFSPANIVPGISYSPDKML
                     QARLFSYGDAQRYRLGVNHHHIPVNAPKCPVHSYHRDGAMRTDGNLGGTATYFPNSRG
                     EWTDQPQLNEPPLVIEGAAAHWDHRVDDDHYQQPGDLFRKMSPAQRQTLFDNTARQVG
                     GAAKHIQERHIANCAKADPAYGAGVAKALGIDFKSQMIAAE"
     gene            2665..>2938
                     /locus_tag="VGA15_10475"
     CDS             2665..>2938
                     /locus_tag="VGA15_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX9588154.1"
                     /translation="MVISSLANRIAPKLLAEFIGTFSFVFIGAGAAAVVGDGVGLPGI
                     VAIALAHGLAIIAFAYGSVSGGHMNPAVTVGVLAAGAMSAGEAIGYI"
BASE COUNT          579 a          963 c          859 g          537 t
ORIGIN      
        1 cgtaagcaag cggcactcgt ttttacttca ggctatgtgt caaaccagac cggaatcgcg
       61 accatcgcca agctcattcc ggaatgcctg atcctgtccg atgagctcaa ccacaattcg
      121 atgatcgaag gcgtgcgcca atccggttgc gagaagcaga tctggcgcca caacgacgtc
      181 gagcatctcg aacgattgct gcaggcggcc gggcagcatc ggccgaagct gatcgtattc
      241 gagaccgtct attccatgga tggcgacgtg gcgccgatgc attgcatctg cgatctggct
      301 gagcgctatg gcgccatgac ctatgcggac gaggtgcacg ccgtcggcat gtacggcgcg
      361 cgtggcgctg gcgtggcgga gcgcgacggc gtgctgcacc ggattgacgt gctggaaggc
      421 acgctcgcca aggcattcgg ctgcatgggc ggatacattg cgggcaacag cgccctgatc
      481 gacgcggtgc gctcgtatgc gccgggcttc atctttacga ccgctttgcc gccgcccatc
      541 tgcgctgctg ccaccgccgc catccgtcac ttgaaggcgt cgaattggga acgccagcga
      601 catcaggaac gagccgcgcg ggtcaaagcc gtactcaacg ccgccgcgct gccggtcatg
      661 ccgagcgaca cccacatcgt gccggtcctg gttggggatc cggagaggtg caaggcggcg
      721 agcgatctgt tgctcacaga gcacggcatt tacatccagc cgatcaatta tcctaccgtg
      781 ccgcgcggat tggagcggct gcggatcaca ccaagcccct accacgacga cgcgctcgtg
      841 gatgctctcg ccgaggccct cttggatgtg tgggcaaggc tcgggctagc gccgaagcgc
      901 tgggcaacgg cagctgaata acctccggct tccggagatt gaagcaaaac cggtgcaatc
      961 gcagcaatag agccaaagtc atcgcgaagc ggatggatac atttgcattt cgcgcacgcc
     1021 tggcatcgat caccgcattg gccggtccgg ccgccgtgat gatccattca accatatgac
     1081 cctaggagca atccaatgaa cgaccaatcc aaacgaccgc cgctgaccca tgcatcgggc
     1141 acgccggtca cggacaattt gaacatccag actgccgggc cgcgcggtcc tgccctgttg
     1201 caggatgtct ggctgcttga aaagctggcc catttccacc gcgaagtgat ccccgagcgc
     1261 cggatgcacg ccaagggctc cggcgcccac ggtaccttca ccgtcacgca cgacatcact
     1321 cgctacacca aggccaagat cttttcgcag atcggcaagc agaccccgat gttcgcccgc
     1381 ttctccacgg tcgcaggcga gcgtggcgcc gctgacgccg agcgtgacat ccgcggcttc
     1441 gcgctgaagt tctataccga agaaggcaac tgggacgtcg tgggaaacaa cacgccggtg
     1501 tttttcttcc gcgatccgtt gcgttttccc gatctcaacc acgccatcaa gcgtgatccg
     1561 cgaaccggta tgcgcagcgc tgacaacaac tgggacttct ggacgctgct gcccgaagcg
     1621 ctgcatcaag tcacgattgt catgagcgag cgcggtattc cccggagttt tcgccacatg
     1681 catggtttcg gcagccacac ctacagtttc atcaacgccg ccaacgaacg cgtctgggtc
     1741 aaattccact tccgcaccca gcagggcatc cagaacctga cggacgcgga agccgaggcc
     1801 cgtgtcggca aggatcgcga aacccatcag cgggatctgt tcgaaaatat cgaagccggc
     1861 aattttccac gctggaccct gttcatccag gtgatgacgg acgctcaggc gaaggctttc
     1921 ccttacaacc ccttcgacct gaccaaggtc tggccaaagg gcgacttccc gctgatcgag
     1981 gtcggatatt ttgagctaaa ccgaaacccc gaaaacgtct tcgccgaagt cgaacaggcg
     2041 gcgttttctc ctgccaacat cgtaccgggc atcagctatt cgcccgacaa gatgctgcag
     2101 gcgcgcctct tctcctatgg agatgcgcag cgctaccgcc tcggggtcaa tcaccaccac
     2161 atcccagtca acgctccgaa atgtcccgtc cacagctatc accgcgatgg cgcaatgcgc
     2221 acggacggca atctcggcgg aactgctacc tacttcccca atagccgggg cgagtggacc
     2281 gaccagccgc agcttaacga gccgccgctg gtgatcgaag gtgcggccgc gcattgggac
     2341 catcgtgtcg acgacgatca ctaccagcag cctggcgatc tcttccgcaa gatgagcccg
     2401 gcgcagcgtc aaaccctgtt cgacaatacg gctcgccaag tcggcggcgc ggccaagcac
     2461 attcaggagc ggcacatcgc caactgcgcc aaggcggatc cggcgtacgg cgcaggcgtc
     2521 gcgaaggcgc tgggcattga ttttaaatcg cagatgattg ccgcggagta ggcctgttcg
     2581 cttcgccggc cggcggcgct tcccagtgcc gtcggccact cccaacaaac gtatcgttcc
     2641 ctggcgctca cctgatcgcg ccgaatggtc atctcatctc tggctaaccg gattgcgccc
     2701 aagctccttg ccgagttcat cggcaccttc tcgttcgtgt tcatcggcgc cggcgcggcg
     2761 gccgtggtcg gcgatggtgt cggcctgccc ggtatcgtcg cgattgcgct cgcccacggc
     2821 ttggccatca tcgccttcgc ctacggctcc gtgtcgggcg ggcatatgaa cccagccgtc
     2881 actgtcggcg tgctcgccgc cggcgcgatg agtgccggcg aggcgatcgg ctacatcg
//
LOCUS       DASWGM010000361         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1701958,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000361 DASWGM010000000
VERSION     DASWGM010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701958"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /gene="mscL"
                     /locus_tag="VGA15_10480"
     CDS             <1..362
                     /gene="mscL"
                     /locus_tag="VGA15_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX9588155.1"
                     /translation="VGVIIGAAFGGIVTSLVGDIIMPIIGAITGGLDFSNYFTGLSKA
                     VTAGNLADAKKQGAVLAWGSFLTLTLNFIIIAFVLFVVIRFMNKLKRRDEAAPAPPKP
                     SREEELLTEIRDLLKKS"
     gene            429..902
                     /gene="smpB"
                     /locus_tag="VGA15_10485"
     CDS             429..902
                     /gene="smpB"
                     /locus_tag="VGA15_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX9588156.1"
                     /translation="MAEKNERAIKVVAENRKARFNYAIEDTVEAGIALTGTEVKSIRN
                     GKTTIAESYADTKGGEIWLINANIPEYLQANRFNHEPKRPRKLLLHRKQINKLMGAVD
                     RDGMTLIPLKLYFNERGRAKLLLAVAKGKKLHDKRESEKKRDWGREKGRLLRARG"
     gene            1068..1643
                     /locus_tag="VGA15_10490"
     CDS             1068..1643
                     /locus_tag="VGA15_10490"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9588157.1"
                     /translation="MNQRNLLEVDWSKIPAPADDGAAAHLEGMTIPSVSLVATDDSSV
                     TLSALPGRTVVFAYPRTGEPGKIALVDDWDMIPGARGCTPQTCAFRDLHAELKAAGAG
                     HVFGLSTQSNDYQAEMASRLHLPFPVLSDEKLTLRRALDLPTMQVAGLTLMKRLALIV
                     DDARITHVFYPVFPPDRSAGDVLAWLKENPV"
BASE COUNT          351 a          505 c          566 g          308 t
ORIGIN      
        1 cggttggcgt catcattggc gcggcgttcg gcggcatcgt cacttcgctg gtcggtgaca
       61 tcatcatgcc gatcatcggc gcgatcacgg gcgggttgga tttctccaac tatttcaccg
      121 gactgtcgaa ggcggtcacc gccggcaatc tggccgacgc gaaaaagcaa ggcgcggtat
      181 tggcctgggg cagtttcctc acgctgacgc tgaacttcat catcatcgcc tttgtgctgt
      241 tcgtggtgat tcgttttatg aacaagctga agcgcaggga tgaagccgcg cccgcgccgc
      301 cgaagccgag ccgcgaggaa gaactgctca ccgagatccg cgatctgctc aaaaagtctt
      361 gacgtgcgcg cacgcgggcc gagtgttacc gctctccgtc ggagatagat tccaaggtat
      421 ttttcatcat ggccgaaaag aacgagcgcg cgatcaaggt cgtcgccgaa aatcgcaagg
      481 cccggttcaa ctatgcgatc gaggacacgg tggaagcggg gatcgcgctg accggcaccg
      541 aggtcaagtc gatccgcaac ggcaagacca ccatcgcgga atcctatgcg gataccaagg
      601 gcggcgagat ctggctgatc aacgccaata ttccggaata tctgcaggct aaccgcttca
      661 accacgagcc gaagcggccg cgcaagctgc tgctgcacag gaagcagatc aacaagctga
      721 tgggtgcggt cgaccgcgac ggcatgacgc tgattccgct aaagctctat ttcaacgaac
      781 gcggccgcgc caagctgctg ctggcggtgg cgaagggcaa gaagctgcac gacaagcgcg
      841 agagcgagaa aaaacgcgac tggggcaggg agaaggggcg cctgctgcgg gcgcggggat
      901 agagtgccgg ccctcatccc tgcgtaacgg cttcgccgtt atcgctggag gagcgcgttc
      961 ttacgcgcgt ctcgaaggat gagaccggtg gcttcatggt tcgagacggc gcaagggcgc
     1021 ctcctcacca tgaggtccga atgtgttgga cgtcgaggga agctgcgatg aaccagagaa
     1081 atctgcttga agttgactgg agcaaaatcc cggcgcctgc cgacgacggt gcggccgcgc
     1141 atcttgaggg catgacgatc ccgtcagtca gcctggttgc caccgacgac agttcggtca
     1201 cgctgtcagc gctgccgggg cgtaccgtgg tgttcgccta tccccgaacc ggcgagcccg
     1261 gcaagatcgc actggtcgat gactgggaca tgatccccgg cgcgcggggc tgcacgccgc
     1321 aaacctgtgc gttccgcgac ttgcacgcgg aattaaaggc ggcgggcgcc gggcacgtgt
     1381 tcggcctgtc gacgcaaagc aacgactacc aggccgaaat ggcctcgcgg ctgcatctgc
     1441 cgttcccggt tttgtccgac gagaagctaa cgctgaggcg tgcgctggat ttgccgacca
     1501 tgcaggtggc cgggctgacc ttgatgaaac ggctggcgct gatcgtcgac gacgcgcgca
     1561 tcacccacgt gttttatccg gtgtttccgc cggatcgaag tgccggcgac gtgctggcgt
     1621 ggctgaaaga aaatccggtt tgatctgtag ggtgggcaaa gccaacgggt cacgcgaatg
     1681 cgcgcccgat gacaggctct gcgtgcccac cttgacgccg gcaacagcgg
//
LOCUS       DASWGM010000362         6936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2395258,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000362 DASWGM010000000
VERSION     DASWGM010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395258"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VGA15_10495"
     CDS             complement(<1..963)
                     /locus_tag="VGA15_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HEX9588158.1"
                     /translation="MSSNAFFSDLLASISERGRTLLRRAGSSDTKRHASDLIELCEAL
                     LSGRGEASGTAMAREVLDHYHNLDEAGRLAFFETLTRDFGPDREKLAEAIEAWRAQPT
                     DADASDLHFASEPRRQELIRQLNRAPGGTSALVAMRADLLRAMNGHKDLAALDRDVVH
                     LLSSWFNRGFLVLRRIDWSTPANILEQIIRYEAVHEIRDWDDLRRRIDPIDRRCYAFF
                     HPALVDEPLIFVEVALTESIPGAIAPLLAEDRRPVPVERARTAVFYSISNTQRGLGGI
                     SFGNFLIKQVVEELRRELPKLENFVTLSPVPGFMKWLKQASDVPV"
     gene            complement(1262..2062)
                     /locus_tag="VGA15_10500"
     CDS             complement(1262..2062)
                     /locus_tag="VGA15_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9588159.1"
                     /translation="MNIAETPIAAIRGSHRATGDGVHLVARHYRKGVRLDTHMHREAQ
                     LVYAAKGTMQVTTPKGRWLVPPDRAVWVPARLEHSIDVLADIEMRTLYFDLAWLARED
                     RSESLDSEFVVRVSRLLHETILALFDGHGNPDRTGLLVRLAVLELHHAEDSATFIPLP
                     HEPRCRRAADIVLGDPTAAHEIETLAREVGTSARTLSRLFSSETQLSFKSWCQRARIA
                     AAIERLSMRADVSVKQLASDLGYASVPAFSHAFRQVTGKTPTEFSETK"
     gene            2125..3300
                     /locus_tag="VGA15_10505"
     CDS             2125..3300
                     /locus_tag="VGA15_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9588160.1"
                     /translation="MNSPIRVIGFVNAAHFIDHYSMLIFAAAVIVMGPALGMAYSELL
                     PYATPGFVAFGAGSLLTGWLGDRWSRRHMMLIFFAGIGASMIAVGLVQTPLQLGAALL
                     AIGLFASIYHPVGTAMIVSYADRLGREMGLNGVWGNLGVASSALVTGVVGQYLGWRWA
                     FIIPGIVTILIGLAFARTVVHEDRSGFRQAAAQARVAKDDMWRVIVALLIVVIAVSTT
                     FNAVTVALPKLFAERLSDLTKSPALLGVIAACVYVFGAMTQYTIGKLIDRYSLKTVML
                     PLSFMLAPFLYLAATLSNLPLILASIGIVMGAFGQVTVNDAMVGKYTSEEWRSRAYAV
                     RYFIGFTAAGVSVGLVAWLYQQGGFVTMLHAFGALCLLVIAAAIILPQEIKVPAVAH"
     gene            3567..4310
                     /locus_tag="VGA15_10510"
     CDS             3567..4310
                     /locus_tag="VGA15_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9588161.1"
                     /translation="MAVPASADPLRDEIAPTGKLRAAIAISTAGGAFWSTKSEAGGYA
                     GVPVDLGKEMATQLGVPVEYVAYDNSGQITDAAAKGIWDVTFLPQDAVRMTKMGFGPI
                     YQVTDATYIVKAGSSVTNFQTLDQPGIKVAAEYNTTTMRGAVAHLKNAKVIEYQTYDE
                     IFALLKNGEIDAFALSRDQLNDMAKKIPGTRVLDETFKQTVTAVAVPLDHPLALAFVN
                     KFMTDAISNGMLRRAYDNNGLKDNPIRVK"
     gene            complement(4330..5127)
                     /locus_tag="VGA15_10515"
     CDS             complement(4330..5127)
                     /locus_tag="VGA15_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9588162.1"
                     /translation="MKSISANIRSLVIGVVGALLLAGSANAAEVRVMISGGLTAAYKA
                     LVPEFERLTGNKVLTAYGPSMGTTVNAIPVRLERGEPADVLIMVGYALGDLVKQGKVI
                     ADSRVDLVKSPIGIAVKSGAPKPDISTADAVKRALLAAKSIAYSDSASGVYVSTEMFE
                     KLGIADAMKDKAKKIPATPVGEIVAHGDAEIGLQQISELKPVEGIDIVGSLPDDLQKI
                     TVFSAGIATVSKEPDAGKALIKFLASPTASAVIVKSGLEPIPVGATN"
     gene            complement(5383..6606)
                     /gene="phaZ"
                     /locus_tag="VGA15_10520"
     CDS             complement(5383..6606)
                     /gene="phaZ"
                     /locus_tag="VGA15_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX9588163.1"
                     /translation="MMSMMYQAYQNHMDLTAPWRTGAASALKYLNLVPQGVSDKLFGR
                     LAAALELISRTSLTYTRPPYGIDKVLVGNQELEVTEEVVYATPFGSLLHFKKENSPEQ
                     PRMLLVAPMSGHFATLLRGTVKTLLQDHDVYITDWHNPRDIPLDQGKFGLDEYTEHLI
                     TFLDQLGPRAHMVAICQPSVSALAAAAIMSEDNHPARPATLTLMAGPIDTRIQPTKVN
                     DFAKSKPLKWFEENLINYVPFQCKGAFRQVYPGFIQLTAFVSMNLERHIKSHVDLADH
                     LAKGEKEKAEIIKTFYDEYFAVMDLPADFYIETIRDVFHEHLLPLGKLTYRGRPVSPA
                     AIKRMGLMTVEGEKDDICSIGQTLAAQDLCTGVRAYRKVHHMQAGVGHYGVFSGRRWN
                     NEIYPLLRDFVHVNS"
     gene            6770..>6936
                     /locus_tag="VGA15_10525"
     CDS             6770..>6936
                     /locus_tag="VGA15_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9588164.1"
                     /translation="MAKATLTISSKNYSSWSLRGWLLTKFSGLEFEEIITAPDDASAR
                     AEILLLSSSILV"
BASE COUNT         1308 a         2101 c         2179 g         1348 t
ORIGIN      
        1 caccggaaca tcgctcgcct gcttcagcca tttcatgaag cccggcaccg gcgacagcgt
       61 cacgaaattc tccagcttcg gcaattcgcg ccgcaattcc tcgaccacct gcttgatcag
      121 gaagttgccg aaagaaattc caccgagacc ccgttgcgtg ttggagattg aatagaacac
      181 cgcggtgcgg gcgcgctcga ccggcaccgg ccgccggtct tcggcaagca atggcgcgat
      241 cgcgcccggg atcgattccg tcaacgccac ctcgacgaag atcaggggct cgtcgaccag
      301 ggcgggatga aagaacgcat agcagcgccg gtcgatggga tcgatacggc ggcgcagatc
      361 gtcccagtcg cggatctcat gcaccgcctc gtagcggatg atctgctcca ggatgttggc
      421 gggcgtcgac cagtctatcc tgcgcagcac gagaaacccc ctgttgaacc aagacgacaa
      481 cagatgcacg acgtcgcggt cgagcgcggc aaggtccttg tggccgttca tggcgcgcag
      541 caaatcggcg cgcatcgcga cgagcgcgct ggtgccgccc ggcgcgcgat tcagctggcg
      601 gatcaattcc tggcgccgcg gctcggaggc aaagtggaga tcgctggcgt cagcgtcggt
      661 cggttgcgcc cgccaagcct cgatcgcctc ggcaagtttt tcccggtccg gaccgaaatc
      721 gcgggtcaag gtttcgaaga atgcaagccg gccggcctca tcgagattat ggtaatggtc
      781 caggacctcg cgcgccatcg ctgtgccgga ggcctcgcct cgcccggaca agagcgcctc
      841 gcacagttcg atcaggtcgg aggcgtgccg cttggtgtcg gatgaccccg cgcggcgtag
      901 caacgtgcgg ccgcgctcgg aaattgacgc gagaagatcg gagaagaagg cgttgctgct
      961 catgtcgggc gggtcaaatc caggctaggc ggtaacatta catggattta aggcgaagcg
     1021 gatcacaatc aagcgggcat ttagcgcaaa aaacgtcttg cctggcgaag tcaaggccgt
     1081 catggccggg catagtagtc tgccttgcgc agactgcgta aacttgtcgg cgctcccggc
     1141 catccatctt gttcgaaaaa tacgcttaag tgaagatgga tagatacgcg ggtcaagccc
     1201 gcgtatgaca gcggagaatg tcgatgcgca ttgcccgcca tccgtcacca ccgaatcggc
     1261 cttatttcgt ctccgaaaac tcggtcggcg tcttgccggt gacctgccgg aatgcgtgtg
     1321 aaaacgccgg cacactcgca tagccgaggt ccgaggcgag ctgtttgacc gagacatcgg
     1381 cccgcatcga caatcgttcg atcgccgctg cgatgcgcgc gcgttggcac cagctcttga
     1441 agctgagttg cgtctccgat gaaaacagcc gtgacagcgt ccgcgccgac gtgccgacct
     1501 cgcgtgccag cgtctcgatc tcatgcgcag ccgtggggtc gccaagcacg atatccgccg
     1561 cgcgccggca gcgcggctca tgcggcagcg gaatgaacgt cgcggaatcc tcggcgtgat
     1621 gcagctcgag gacggccagc ctgaccagaa gcccggtgcg gtcagggtta ccgtggccgt
     1681 cgaacagcgc gaggattgtc tcgtgcagca gccgcgagac cctgacgacg aactcggaat
     1741 ccaggctttc gctgcggtcc tcgcgcgcca gccatgccag atcgaaatac agcgtccgca
     1801 tctcgatatc cgccagcacg tcgatggaat gttcgagacg ggcgggtacc cagaccgcgc
     1861 ggtccggcgg caccagccag cgccccttcg gcgtcgtgac ctgcatcgtg cccttggcgg
     1921 cgtacaccag ttgcgcctcg cgatgcatgt gggtgtcgag ccggacgccc ttccggtaat
     1981 ggcgcgcgac cagatggaca ccgtccccgg tggcgcggtg gcttccgcgg atggcagcga
     2041 ttggcgtttc ggcgatgttc attgcccaca tcccgtcagg acaagaccct ataacctatt
     2101 tcaggaaatc aagggactag gtctatgaac agccctatca gggtgatcgg tttcgtcaac
     2161 gccgcccact ttatcgacca ttattcgatg ctgatcttcg ccgccgcggt tatcgtgatg
     2221 gggccggcgc tcggcatggc ctattcggag ctgttgcctt acgccacccc gggcttcgtc
     2281 gcgttcggcg ccggatcgct gctgaccggc tggctcgggg atcgctggag ccgccgtcac
     2341 atgatgctga ttttctttgc cggtataggg gcatcgatga tcgcggttgg tctcgtgcag
     2401 acgccgctgc agcttggcgc ggccttgctc gcaatcgggc tgttcgcatc gatctaccac
     2461 cccgtcggca ccgcgatgat cgtgtcctac gccgaccggc tcggccgcga gatgggcctg
     2521 aacggcgtct ggggcaatct cggcgtggcg tcgtcggcgc tggtgaccgg tgtcgtcggc
     2581 cagtatctcg gctggcgctg ggcgtttatc attcccggca tcgtcaccat cctgatcgga
     2641 ttagccttcg cccgaactgt ggtccacgag gatcgctccg gcttcaggca ggccgcggcg
     2701 caggcgcggg tggcgaaaga cgacatgtgg cgggtgatcg tggcgctctt gatcgtggtg
     2761 atcgcggtat cgactacctt caacgcggtc accgtggcgt tgccaaaact ctttgcggag
     2821 cgcttgagcg acctgaccaa gagcccggca ctgctcggcg tgatcgccgc ttgcgtctat
     2881 gtgttcggcg cgatgaccca atataccatc ggcaagctga tcgaccgcta ttcgctgaag
     2941 accgtcatgt tgccgctgtc cttcatgctg gcgccgttcc tgtatctggc ggccacattg
     3001 tccaacctgc cgctgatcct ggcttcgatc ggcatcgtga tgggggcgtt cgggcaggtc
     3061 accgtcaacg acgccatggt cggcaaatac accagcgagg aatggcgctc gcgcgcctat
     3121 gcggtgcgct atttcatcgg cttcaccgcc gcgggggtgt cggttggact ggtggcgtgg
     3181 ctttaccagc agggagggtt tgtcaccatg ctgcatgctt tcggggcgct gtgcctgctg
     3241 gtgatcgcgg ccgcgatcat cctgccgcag gagatcaagg tgcccgcggt agcgcactga
     3301 ttccggcaac gtgcagaagc agttgaactt tgcgcgacca tcgccgctgg caggtcttga
     3361 cgtgccgaca ccgcgcttga tccgacccgg ggtactgcca acaaaattta tcaatcccgc
     3421 cagagaatcc gggtttcaac ggctcccgca atcgaccact atggccggcg aaggctcgac
     3481 cataacgagc ccatgggtgg agaattcgga tgtccaatca atcctggccg cgcatggccg
     3541 gcctcgttct gggtgtttca atcatgatgg cggttcccgc aagcgccgac cccctcaggg
     3601 acgaaatcgc gccgaccggc aagctgcggg cggcgatcgc gatcagcacc gcgggcggcg
     3661 cattctggtc caccaaaagt gaggccggcg gttatgccgg ggtgccggtc gatctcggca
     3721 aggagatggc gacgcagctc ggggtccccg tcgaatatgt cgcgtacgac aattccggac
     3781 agatcaccga cgctgcggca aaaggcattt gggatgtgac gttcctgccg caggacgccg
     3841 tgcgcatgac caagatggga ttcgggccga tctatcaggt cacggatgcg acctacatcg
     3901 tcaaggcggg ctcgtcggtg acgaattttc agacgctcga tcagcccggc attaaggtcg
     3961 cggcagaata taacaccacc accatgcgcg gcgcggtcgc gcatctaaag aatgcgaaag
     4021 tgatcgaata tcagacctac gatgagatat ttgcgttgct caagaacggc gagatcgatg
     4081 catttgcgct gtcgcgcgac cagctcaacg atatggcaaa aaagattccc ggaacgcgcg
     4141 tgctggatga aaccttcaag cagaccgtga ccgcggtggc cgtaccgctc gatcatccgc
     4201 tggcactggc cttcgtgaac aagttcatga cggacgcaat ctcgaacggc atgctgcggc
     4261 gcgcctacga caacaacggc ctgaaggata atccgatccg ggtgaaatag cgcggggtct
     4321 caagctccgc taattcgtgg caccaaccgg gatcggctcc aggccgctct tgacgatcac
     4381 agcgctggcg gtgggcgagg caagaaattt gatcagcgcc ttgccggcgt cgggctcctt
     4441 ggacacggtc gcgatcccgg ccgagaaaac cgtgatcttc tgcaagtcgt cggggaggga
     4501 accgacgata tcgatgccct ctacgggctt gagctcgctg atctgctgca ggccgatctc
     4561 ggcgtcgccg tgcgccacga tttcgccgac cggggtggca ggaatttttt tggccttgtc
     4621 cttcatcgca tcggcaatgc cgagtttctc gaacatctcg gtcgagacat agacgccgct
     4681 ggctgagtcg gaataggcga tcgatttcgc cgccagcaac gcgcgcttca ccgcgtcggc
     4741 tgtactgatg tccggcttcg gcgcgcccga cttcaccgcg atgccgatcg gcgatttcac
     4801 cagatcgaca cggctgtcgg cgatcacctt gccctgcttg acgagatcac cgagcgcata
     4861 gccgaccatg atcaggacat cggcgggctc gccgcgctcg agccggaccg ggatcgcatt
     4921 gacggtggtc cccatcgagg gtccgtaggc cgtcagcacc ttgttgccgg tcaagcgctc
     4981 gaattccggc accagcgcct tataggccgc cgtcagtccg cccgagatca tcaccctgac
     5041 ctcggcggcg ttcgccgagc ctgccagcag cagggcacca acgacgccga taacgaggct
     5101 gcggatattt gcggatatcg atttcatgat cagtctcccg ggacgactct tgattggtcg
     5161 tcgttgagac gatgctatcc tgcaaacgga ggatagaata gggctcgatc tattgggatt
     5221 aaggcgacgg acgcgcggca acggcagcgc gctccctctc cctttgggag agggttgggg
     5281 tgaggggtta cgatctatcg ataggccgta acccctcacc cggcgctgcg cgccgacctc
     5341 tcccgatggg agaggtaaga gaaaccgaac ttcttcgtca gctcacgaat tgacgtggac
     5401 gaaatcccgc agcagcggat agatttcgtt gttccagcgc ctgccgctga atacgccgta
     5461 gtggccgacg ccggcctgca tgtgatgcac cttgcgatag gcgcgcacgc cggtgcagag
     5521 gtcctgcgcc gcgagcgtct ggccgatcga gcagatatcg tctttctcgc cttcgaccgt
     5581 catcaggccc atgcgcttga tcgcggccgg gctcaccggc cggccacggt aggtcagctt
     5641 gcccagcggc aagagatgtt cgtgaaacac gtcgcggatg gtttcgatat aaaaatccgc
     5701 cggcagatcc atcacggcga aatattcgtc atagaaggtc ttgatgattt cggctttttc
     5761 cttctcgccc ttcgccaaat gatcggcgag atcgacgtgg gatttgatgt gacgctcgag
     5821 gttcatcgag acgaaggcgg tgagttgaat gaatccggga tagacttggc ggaacgcgcc
     5881 cttgcactga aacggcacat agttgatcag gttctcctcg aaccatttga gcggcttgct
     5941 cttggcgaaa tcgttgacct tggtcggctg gattcgggtg tcgatcgggc cggccatcaa
     6001 ggtcagggtc gccggccgcg ccggatgatt gtcctccgac atgatggccg cggcggcaag
     6061 cgccgacacc gacggctggc agatcgccac catgtgcgca cgcgggccca attgatcgag
     6121 aaacgtgatc aggtgctcgg tgtattcgtc gaggccgaat ttgccctgat cgagcggaat
     6181 gtcgcggggg ttatgccaat cggtgatgta gacgtcgtga tcctgcagca gggttttcac
     6241 cgtgccgcgc agcagggtgg cgaagtggcc ggacatcggc gccaccagca gcatgcgcgg
     6301 ctgctccggt gagttttcct tcttgaaatg cagcaacgaa ccgaacggcg tcgcgtacac
     6361 gacttcttcg gtcacctcga gttcctgatt gccgaccagc accttgtcga tgccgtaggg
     6421 cggccgcgtg taggtgagcg acgtgcgcga gatcaactcc aacgccgcgg cgagccgccc
     6481 gaacagtttg tcggagacgc cctgcggcac caggttaagg tatttcagcg ccgatgctgc
     6541 cccagtccgc catggcgccg tcaggtccat gtggttctgg taggcctggt acatcatcga
     6601 catcatccgg atccgcccct gcccgtgttg ctatgcaata acgaagccaa agcggagtca
     6661 attagccttg caaactggca cgtcgcttgc tgaataaacg cttgaagcat aaacactggg
     6721 caaggcgcgg cggccctggc gggcgttcag gcgtgaggga aaaagccata tggcgaaagc
     6781 gacactgacc atcagcagca agaactattc gtcgtggtcg ttgcgcggct ggctgctgac
     6841 gaagttctcc gggctggagt tcgaggaaat catcaccgcg ccggacgatg cttcggcgcg
     6901 agccgaaatc ctgctgctgt cgtcatcgat cctggt
//
LOCUS       DASWGM010000363         3751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2659671,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000363 DASWGM010000000
VERSION     DASWGM010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2659671"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VGA15_10530"
     CDS             complement(<1..639)
                     /locus_tag="VGA15_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588165.1"
                     /translation="MPDLTDSVECVRGGFWRRALAVFIDLIAISALLQVVALVLFPLT
                     HGQVQFSGGPLHGLNCQKLASVPEGVSIPPEFNANSVTDCQQSLLGLPTARTLRVSRI
                     TKDGAVTKVAQIRQRLDAEGKPTPGLPLDIFVLPLLIALRFALDRAGGSIGRRICRLR
                     LSNASDGRTPPPTTSVSRRYAALALPLAPAWIWSAYAALFPGQELLGTTLYWL"
     gene            894..1334
                     /gene="aroQ"
                     /locus_tag="VGA15_10535"
     CDS             894..1334
                     /gene="aroQ"
                     /locus_tag="VGA15_10535"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX9588166.1"
                     /translation="MKRLMILNGPNLNLLGVREPHIYGTTTLAQIEQDCVRHAADLGL
                     TLTFRQSNHEGELVDLIQSARQDADAIIINPAGYSFTSIAILDAIKAFEGPVIEVHIS
                     NIHARDEHHRHSKISSVASGVICGLGPYGYIAAMHALAQMAKKA"
     gene            complement(1363..1599)
                     /locus_tag="VGA15_10540"
     CDS             complement(1363..1599)
                     /locus_tag="VGA15_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1902 domain-containing protein"
                     /protein_id="HEX9588167.1"
                     /translation="MSRPVIFTISAAWDQEASVWTGHCDDIPAASDAPTLDELLEKIS
                     AMALDLLPDNHPDVAPESLFLQITALREAEPAAN"
     gene            complement(1702..2139)
                     /locus_tag="VGA15_10545"
     CDS             complement(1702..2139)
                     /locus_tag="VGA15_10545"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX9588168.1"
                     /translation="MAEIRHQIAIEAPPEKVYAALATQAGLRSWWTADTVADHKTGGK
                     AEFGFAQYRVKPSGVDKVKRAIAEFVPYVRANEPGTKLYQAWQQKDDPTRFVHFFIFE
                     DEAAHAAHAGSEAVKRFEAAYRPELSEGDVVFTDYQLVAANRR"
     gene            2299..3633
                     /locus_tag="VGA15_10550"
     CDS             2299..3633
                     /locus_tag="VGA15_10550"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588169.1"
                     /translation="MSGRAETLIRNWETRLHANALASQILATLNERKSEVQRCALDGL
                     ARENPQFERATTKQFREEAVGHCDTILQTMLAIATGRAATLGPDPFAFVQAHAVRRAR
                     QQFPLAGSLNAYRLAHKAYWEVMRNSAADAMVPESEKTDCLMILSEFLLEFFDRISGI
                     MTDAYIAEEKRMIARSTRAHVALIEDLLHGRQPGDLEAQGLSERCGMHSGSPIAVVIG
                     RISHSGNGRFHGAGKLERLSGFFHRALSPLAFGRLIDIRKDEVLAILTAGAQTSKRVR
                     QAFAAIAAELKAELNDLARIGISLDATEIAALPQGYQEAERAVEFAKANRPILHFADI
                     DLMEFLVRRPDAAALRGGGTAVAQTASKSDSKCSQQNRLYCETAFFPAVPSNRDQPTL
                     EPQGRTDQADPPIPAGSFGCRLFEHWTSSAERIRLLASSPKVRSHPSFANSF"
BASE COUNT          748 a         1190 c         1113 g          700 t
ORIGIN      
        1 cagccagtac agcgtggtgc cgagcagctc ctggccggga aacagtgccg cataggccga
       61 ccagatccag gccggcgcca gcggcaacgc cagcgccgcg tagcgccggc tcaccgacgt
      121 cgttggcggc ggggtgcgtc catccgatgc gttcgacaga cgaaggcggc atatcctgcg
      181 gccgatactt cccccggcac ggtcgagcgc gaagcgcaac gcgatcaaca gggggagaac
      241 gaaaatgtcg agtggcaaac cgggcgtcgg cttgccctcc gcgtcgagcc tttggcggat
      301 ttgcgcgacc ttggtcacgg cgccgtcttt ggtgatccgg ctgaccctca aggtgcgggc
      361 tgtcggcagc cccagcaggc tctgttgaca atcggtcacc gaattggcgt tgaattcggg
      421 cgggatcgac actccctcgg gcactgacgc cagtttctgg cagttgaggc catggagcgg
      481 gccaccggaa aactgcacct ggccgtgggt caacggaaac agcaccagcg cgaccacctg
      541 caaaagcgcg ctgatcgcga tcagatcgat aaaaaccgcc agcgcccgcc gccagaatcc
      601 accccgaaca cattcgacgc tgtctgtcag gtccggcaaa atcaaccctt gaaaaatgca
      661 ggtgtcggga tcggaagaac cggcatcctg cgtcgctcgt cccgggtgcg atgctgcccc
      721 ggatcggttg aactgacaag tcgccgcaac cggttttcag aagtgaggtc tctccgtttc
      781 gcccccgatt gcgccacgcg ccatgcgcgc tacggtattg gccggttccc atatgctcac
      841 gaccgaatct gccgaaacgg ctcgacggct aaacccctaa ggagatggac gccatgaaac
      901 gactgatgat cctgaacggt cccaatctca atctgttggg ggtgcgggaa ccccatatct
      961 acgggaccac cacgctggcc cagatcgagc aagactgcgt ccgccacgcc gcggacctcg
     1021 gccttaccct cacctttcgc cagtcgaacc acgaaggcga gctcgtcgac ctgatccagt
     1081 cggcacggca ggatgcggac gccatcatca tcaatccggc cggttattcc ttcacctcga
     1141 tcgccatcct cgatgcgatc aaggcgttcg aagggccggt gatcgaagtg cacatttcca
     1201 acatccatgc ccgcgacgaa catcaccggc attcgaagat ctcgtcggtc gcctcaggcg
     1261 tgatctgcgg gttgggaccc tacggctaca tcgccgcgat gcacgcgctg gcgcagatgg
     1321 cgaagaaggc gtgagcggcg tccgacgcga ctgctgcgcc attcagttcg cggccggttc
     1381 ggcttcgcgt aacgcggtga tctggaggaa cagcgactca ggcgcgacat ccggatggtt
     1441 gtcgggcaac aaatcgagcg ccatcgcgga aatcttttcc agcaattcgt cgagtgtcgg
     1501 agcgtctgag gcggccggaa tatcgtcgca atgcccggtc cagaccgacg cctcctgatc
     1561 ccaggcggca gaaattgtga aaatgacggg tctggacatt tccgctccat tctctgtcgc
     1621 gacgcgcctc aagacgcacg cgggcacgtg cattatatca caaatccggc gggacgtccc
     1681 gcgtttcgag agaggccccc ctcacctccg gttggccgcc accagttgat aatccgtaaa
     1741 gacaacatcg ccttccgaaa gctccggccg ataggcggct tcaaatcgct tcaccgcctc
     1801 cgagccggca tgggcggcat gcgccgcctc gtcttcaaaa atgaaaaaat gcacaaagcg
     1861 cgtcggatcg tccttctgct gccatgcctg ataaagtttg gtgccgggct cattggcgcg
     1921 gacatagggg acgaactccg cgatcgcgcg cttgaccttg tcgacgcctg agggtttgac
     1981 gcggtattgc gcgaagccga actcggcttt cccgccggtc ttgtggtcgg caacggtatc
     2041 cgcggtccac catgaacgca aacccgcttg cgtggcgagc gcggcgtaga ccttttccgg
     2101 cggtgcctcg attgctatct ggtgcctgat ctcggccatt tgattttatt ccctcacatt
     2161 tgcgaggttt ttgcgaacgc tgaatcagtc gagcacttgc agcctaggca catcaggcgt
     2221 tttgactatt gccaatcagc catattaatc tcggctggtt tttgtgaaat ttcacaaccg
     2281 aaaaaagcgg agccagccat gtccggtcgc gccgaaacgc tgatcaggaa ctgggaaacg
     2341 cggctgcacg ccaatgcgct ggcctcgcag attctcgcca cgctgaacga gcgaaaatcc
     2401 gaagtgcagc gctgcgcgct cgacggactg gcacgcgaaa atccgcaatt cgagcgcgct
     2461 accacaaaac aattccgcga ggaagcggtc ggccactgcg acaccatcct gcagacgatg
     2521 ctcgccatcg cgaccggccg ggcggccacg ctcggccccg atccgtttgc cttcgtgcag
     2581 gcgcatgcgg tgcgccgcgc gcgccagcaa tttccgttgg ccggctcgct caacgcctac
     2641 cggctggcgc acaaggccta ttgggaggtg atgcgcaact cggcggcaga cgcgatggtc
     2701 ccggagagcg aaaagaccga ttgcctgatg atcctgtccg aattcctgct ggagtttttc
     2761 gaccgaatca gcggcatcat gaccgacgcg tatatcgcgg aggaaaaacg catgatcgcg
     2821 cgcagcacgc gcgcgcatgt ggcgctgatc gaggacctgc tgcacgggcg gcagcccggc
     2881 gatctcgagg cgcaaggttt aagcgagcgc tgcggcatgc attcgggctc gccgatcgct
     2941 gttgtgatcg ggcgtattag ccattccgga aatggccggt tccacggcgc cggaaaactc
     3001 gaaaggctct cgggtttttt ccatcgagcc ttgtcgccgc tggcgttcgg tagattgatc
     3061 gatatccgca aagatgaagt gctggcgatc ctgaccgccg gcgcgcagac ctcgaagcgc
     3121 gtgcggcagg cgttcgccgc gatcgccgcc gagctgaagg ccgaactgaa cgatctggcg
     3181 cggatcggca tcagccttga tgcgaccgaa atcgccgcgc tgccgcaagg ctatcaggaa
     3241 gccgaacgcg cggtcgagtt tgcgaaagcc aaccggccga tcctgcattt tgccgacatc
     3301 gatctcatgg aatttctggt gcgccgcccc gatgccgcgg cgctgcgagg cggcgggacg
     3361 gcggtagcgc aaacggcctc gaagtctgac tcgaaatgca gccaacagaa caggttgtat
     3421 tgtgagacgg cgttcttccc cgccgtcccg tcaaatcgcg accaacccac gctcgaacct
     3481 caaggccgga ccgatcaagc cgatccgccg atacccgccg gctcgtttgg ttgccggctc
     3541 tttgagcatt ggacatcgag cgccgagcgg attcgattgc ttgcgagcag cccgaaagtc
     3601 cgctcgcacc cttctttcgc caattccttt tgaatctctt tccagctgtc aaatttgccg
     3661 gtattcgcca gaaaaacagc ccgtcgatat atttcgagtc ttcgcctgcg catcacacac
     3721 ccctgcacac tcaatcggtg gttcggcatc g
//
LOCUS       DASWGM010000364        12676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_868575,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000364 DASWGM010000000
VERSION     DASWGM010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12676
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868575"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..1348
                     /locus_tag="VGA15_10555"
     CDS             503..1348
                     /locus_tag="VGA15_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9588170.1"
                     /translation="MVDYPGRFVWYELMTTEVAVAKAFYAKVVGWSAQDASTPDLAYT
                     LFTVGGASVSGLMELPEDARKMGATPRWMGYVGVDDVDVTADRIKRLGGAVYVPPTNT
                     NIGRISVVADPQTATLALIEGPKKPGRQQPAELDRPGHVGWHELLAADWEKAFAFYGE
                     LFDWQKANAEIGPTNTYQLFAAGGQTIGGMFTKRPVEPVPYWLYYFNVDDIDAASERV
                     KTGGGQIFEGPIEVPGGNWIARCIDPQGAIFALQGKRSQDGFGWSTEWDGFSSRGRLV
                     TKPRA"
     gene            complement(1379..2533)
                     /locus_tag="VGA15_10560"
     CDS             complement(1379..2533)
                     /locus_tag="VGA15_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX9588171.1"
                     /translation="MKINATLPEKGRDLRLDLFRGVANWAIFLDHIPDNVVNWITTRN
                     YGFSDAADLFVFISGYTASFVYARMMIERGFVVGATRLTKRVWQLYVAHVILFVIYIV
                     SIGYVAQRYSDPDIINEFNVAGLVDNPIETLRQGLLLKFKPVNLDVLPLYIALMGLFP
                     PVLWMMLRQPNLTMLASIALWLAARQFGWNLPAYPAGAWYFNPYCWQVLFVFGSWCAL
                     GGARKSMSIINAPMTLYFCLGYLVLALVMTMAGKFPDFGALFPHWLYDTFNPNDKTNL
                     APYRFVHFVVIVILVIRFVPKNWPGLEWRIFDPVIVCGQQSLAVFCVGVFLSFVGHFE
                     LMMSSGSLFAQIFVSVTGIAIMTIVAYYISWSKRQDKPLPKPAVAPAKAG"
     gene            2752..3057
                     /locus_tag="VGA15_10565"
     CDS             2752..3057
                     /locus_tag="VGA15_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEX9588172.1"
                     /translation="MTARISKPAKNAMQSGAAKTREWQLDYEPEQPRAIEPLMGWTSS
                     GDMKQQLTLRFDSKEEAVAYCEREGIPYQVIEPKEPARRQAAYADNFAFRRGEPWTH"
     gene            3212..4816
                     /locus_tag="VGA15_10570"
     CDS             3212..4816
                     /locus_tag="VGA15_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX9588173.1"
                     /translation="MPLHSSTDPTSSDFAGNAEAMRLLVAELREKLDQVAGGGGETSR
                     ARHTSRGKMLARQRVDLLVDPGTAFLELSPLAADGLYGGDVHSASIVTGVGRISGREC
                     VIVANDATIKGGTYYPMTVKKHLRAQDIARQNNLPCVYMVDSGGAFLPQQDEIFPDER
                     HFGRIFYNQAQMSAAGIPQIAIVMGSCTAGGAYVPAMSDESIIVRNQGTIFLGGPPLV
                     KAATGEVVTAEELGGADVHSRQSGVTDHYAQNDSHAIGIARRIVATLKQPARANLNMR
                     EPREPLFAREEIYGVVPADGRKPYDVRDIIARVVDGSEFDEFKKLYGQTLICGFAHIW
                     GYPVGIIANNGILFSESALKGAHFIELCCQRNIPLVFLQNITGFMVGKKYEAGGIARD
                     GAKLVTAVATAAVPKFTVVIGGSYGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQAA
                     MVLSQLRRDNIEAKDGSWSIEEENEFRAPMRAQFEAQGNPYYATARLWDDGVIDPADT
                     RLVLGLGLSAASNAPIAPTRFGLFRM"
     gene            4819..6825
                     /locus_tag="VGA15_10575"
     CDS             4819..6825
                     /locus_tag="VGA15_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX9588174.1"
                     /translation="MDRSKQYRRFRTLLIANRGEIACRVIRTAKAMGLRTVAVYSEAD
                     RDAMHVAMADEAVLLGPARARDSYLNIDRVIAAARQSGAEAVHPGYGFLSESAEFAQA
                     CADAGLIFVGPTADMIRAMGSKSGSKMLMEKAGVPLVPGYHGEAQDEATLAKSADKIG
                     FPVLVKASAGGGGRGMRIVRSAGELAAAIVSAKREAKAAFGDDRMLIEKFVQNPRHIE
                     VQIIGDSHGNLLSLFERECTLQRRHQKVIEEAPSPTLNARQRETVCAAARKAAAAVNY
                     VGAGTIEFVSDGKDVFFIEMNTRLQVEHPVTELITGIDLVEWQLRVAFGEKLPLMQDE
                     IKLNGHAIEARVYAENPHKNFMPSVGKIRTWRMPEPSNGLRIDAGYREGDAVSPHYDA
                     MLAKVIAWAPNRDAAIERLNRGLEESDVRGIVSNIPFLAALVTHPAVRANAIDTGFIE
                     RELKNLTPPSAAPGDLELCAAVAAILGEEAKAARAEAHSPWRTAGWMPVGRRRRVFTF
                     RHAPEAAQEICLHYGSGPAALSIGEREFTFASSPREDGGFDLTLNGMKSHVVAVIEGH
                     ELYLRTRNGRFDLHWVDPFGGDDEEQVGEDKIVAPLPGTVVALLAEVGARLEKGAPIL
                     TLEVMKMEQTLRAPFAGVLRALKCKVGDIVQEGVELAEVEPIAS"
     gene            6833..7741
                     /locus_tag="VGA15_10580"
     CDS             6833..7741
                     /locus_tag="VGA15_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX9588175.1"
                     /translation="MSDSVRIVEVGPRDGLQNEKTPVSVADRIGFIGALVGAGLHTVE
                     VGAFVSPKAIPQMVNSDQVLQGLSHHNDSEFHVLVPNVKGYEAARAAGSRVIAVFASA
                     SEGFSRANINCSVAESIDRFRPVVARAKADGIKVRGYVSCVLGCPFDGEVKPQAVVDV
                     ARMLWDLGCYEVSLGDTIGIGTPLKARHLLRAVAGDVPIAHLAMHFHDTYGQALANLY
                     AGIEEGARVIDCAAGGLGGCPFAPGATGNVATEDVVYMLEGMGIATGVDMQKLVAATN
                     EVSKLIGRPPVSRVAAAINAKRGVAK"
     gene            complement(7867..9225)
                     /locus_tag="VGA15_10585"
     CDS             complement(7867..9225)
                     /locus_tag="VGA15_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9588176.1"
                     /translation="MTVLGIGISYGIATLVAMFSGMPIAFALGAVAVVFMGIYMPAAS
                     LDTVTQNVYEEMASITLLSIPLFILKGAAIGKSRAGQDLYSALHAWLHRVPGGLGIAN
                     VFACALFAAMAGSSPATCSAIGSAGIPEMRKRGYSGGFAAGIIAAGGTLGILLPPSIT
                     MILFAVAAEKSLGRLFLAGIGPGLLLVSLFGVYAVIHFRKEYAAASALYAKTGATSAI
                     LARDKFTMAERFNVLPRVLPFVLLLTGVMIALYGGYATPSETAGLGGLLALVLIALIY
                     SVWRPSDLAPILKSTIRESTMLMMIIGMSLLYSYVMSYLHISQSVAESIVAMHLPRWE
                     LLAAILVMVVVLGFFLPPVSIILMTAPIILPPLRAAHFDIIWFGIVMTIVMEMGLIHP
                     PVGLNIFVIRNVAPDIPLSEVIWGTLPFVLLMMLAVVVLCFVPEISTSLPNLVMGPDG
                     GR"
     gene            complement(9222..9776)
                     /locus_tag="VGA15_10590"
     CDS             complement(9222..9776)
                     /locus_tag="VGA15_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9588177.1"
                     /translation="MMHGPIKDQNDRSNVAAGRGLVAAFERALAFCNNVIVVFAALAL
                     IAACLVLSHSVLTRALFHSPNYWQDEAAVFLLVGATFMTAAYVQQQRGHIGIEAFVGL
                     LSPMVNRIRLWLVDVASLLFCGFFAWKSWTLAHEAWVDGQVSNSMWSPPLAIPYGLMA
                     SGMTLLCVQILLQIMIPLCGPARR"
     gene            complement(9773..10795)
                     /gene="dctP"
                     /locus_tag="VGA15_10595"
     CDS             complement(9773..10795)
                     /gene="dctP"
                     /locus_tag="VGA15_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9588178.1"
                     /translation="MLTRRYVLASALAAPAILRFGTGTAHAATTLKISHQFPGGTVDK
                     GDFRDRLTRVFADEVAKRSGGDLVAEIYPNSSLIKTNAQFSAMRKGALDISLVPLSYA
                     GGEVPETNIGLMPGLVSTYDQGLRWKNEPVGKALTDFLADKGIIMLTWIWQAGGVASR
                     SRPVVAPEDAKGLKIRGGSREMDMVLQTAGASVLSLPSNELYAAMQTGACDAGLTSST
                     SLISFRLEEIAKALTSGAGASYWFMLEPVLMSKSIFDALPKNQQDIIVAVGTELEAFG
                     KKGAQADDIEVAHVYEKAGAKVFPLDIPTVGKWRDIARDTAWKDYGAKTAAAANLLKL
                     ASDVSA"
     gene            11151..11387
                     /locus_tag="VGA15_10600"
     CDS             11151..11387
                     /locus_tag="VGA15_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide MXKDX repeat protein"
                     /protein_id="HEX9588179.1"
                     /translation="MTISTRFALGISAATLSLSLALAPVAFAQDKMGKDDGMKKDTMS
                     KDTMKKDTMSKDAMKKDDGMKKDGMMKKDDGMKK"
     gene            complement(11482..11826)
                     /locus_tag="VGA15_10605"
     CDS             complement(11482..11826)
                     /locus_tag="VGA15_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HEX9588180.1"
                     /translation="MSGFKEPSFADRQKAAQEARQNILNKFRAKPGLDDPAVRQRQAE
                     REAQAAERAKARLAREAAKAEKIAREAEAAAQATAQLAREKEEAAAREVALEAERKAA
                     RDARYAARKKRK"
     gene            complement(12084..12338)
                     /locus_tag="VGA15_10610"
     CDS             complement(12084..12338)
                     /locus_tag="VGA15_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588181.1"
                     /translation="MREENPDRSEPTAQAVRALLILSPFLFAFCYFLALVQGASEQAA
                     VVIGLGAFGMYLGAAGLNQLRGPKAAGDLWLLVMIMKLIR"
     gene            complement(12316..12531)
                     /locus_tag="VGA15_10615"
     CDS             complement(12316..12531)
                     /locus_tag="VGA15_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588182.1"
                     /translation="MNQQDSEFDYPRYRKLLAEAVDEPRRLALINLLIDERARDKLAA
                     QATQQRSDLIRRMIAPRNEPDARRKSG"
BASE COUNT         2589 a         3814 c         3993 g         2280 t
ORIGIN      
        1 tgcatttctc catatcttgc aaagatcttt agagtcccgc aggttgattg tacgctttgc
       61 agatttccca agcaaggctg cttacacacg ctgggcgcat cgagttcttg gatttcatgt
      121 tcatgaacga tgtgttcata aacgacttcg gcgccgctta tcaaattaca atgatccagc
      181 gcgtccccga agctcaacgc gcgcaggtca ggaacgaccg ccggctcgag acacctctcg
      241 taaccagtct atcaaacaat tcataaattg catcgaagcc agctatcaac aatcaacggg
      301 ccaagccctt cgtccgaaat cacgaccggg gtgtatcgct ttcgttgcgc tggaaagggg
      361 cgccgtgacc atccttgcat tcgctccgcc ccgccgttat tcaggctaaa gtacttagcc
      421 tcacaatcat gcgccggaaa tgaagccaac tcggctcaat atggcgccgc gactgtttgc
      481 tggctcgagc ggagaaaccg acatggtcga ttatcccggc cgtttcgtct ggtacgagct
      541 gatgacgaca gaagtagcgg tcgccaaggc cttctacgcc aaggtcgtgg gctggagcgc
      601 gcaggacgcg tcgacgccag atctggccta caccttgttt accgttggag gcgcctcggt
      661 aagcggtctc atggagctac cggaggacgc gaggaaaatg ggcgcgacgc cgaggtggat
      721 ggggtatgtc ggcgtcgacg atgtcgacgt taccgccgac cgcatcaaac gtctcggcgg
      781 agccgtgtac gtcccgccga ccaacaccaa tatcggccgc atttctgtcg tcgcggaccc
      841 gcaaacggca acgcttgcgt tgatcgaagg accgaaaaaa cccggccgac agcagcctgc
      901 cgaactggac aggccggggc acgtcggctg gcatgagttg ctcgccgccg actgggagaa
      961 ggcgtttgct ttctacggcg agctttttga ctggcagaaa gcgaacgccg aaatcggtcc
     1021 gacaaacacc tatcagttgt ttgccgccgg cgggcagacc atcggcggca tgttcaccaa
     1081 gcgtccggtg gagccggttc catactggct ctattacttc aatgtcgacg acatcgacgc
     1141 ggcctcggag cgcgtgaaga cgggtggcgg ccagatcttc gagggcccga tcgaagtgcc
     1201 gggcggcaac tggatcgcgc gatgcatcga tcctcagggc gccatcttcg cgctgcaggg
     1261 aaagcggagc caggatgggt tcggctggtc caccgagtgg gacgggtttt catcgagagg
     1321 cagactggtc accaagcccc gcgcatagcg ttcggattcg ggaactagct ggcgtcgatc
     1381 acccggcctt ggccggcgcc accgccggct tgggcagcgg cttgtcctgc cttttcgacc
     1441 aggagatgta gtaagcgacg atggtcatga tcgctatccc ggtcacgctg acgaaaatct
     1501 gcgcgaacag cgagcccgaa ctcatcatca gttcgaaatg cccgacaaag gacaggaaca
     1561 caccgacgca gaacacggcg agcgactgct gaccgcagac gatgaccgga tcgaaaatcc
     1621 gccattccag ccccggccag ttcttgggta cgaatctgat gaccaagatc acgatcacca
     1681 caaaatgcac gaagcgatag ggcgcgagat tggtcttgtc gttgggattg aaggtgtcat
     1741 agagccagtg cgggaacagc gcgccgaaat ccggaaattt cccggccatc gtcatcacca
     1801 gcgccagcac gaggtaccca aggcaaaagt agagcgtcat gggcgcgtta atgatcgaca
     1861 tggactttct ggcgccgccg agcgcgcacc aggagccgaa gacaaacagc acctgccagc
     1921 agtacgggtt gaaataccac gcgccggcgg gataggccgg caggttccag ccgaactggc
     1981 gggcggcgag ccacagcgca atcgaggcca gcatcgtcag gttgggctgg cgcagcatca
     2041 tccacagcac cggcggaaac aggcccatca gcgcgatgta aagcggaagc acgtcgaggt
     2101 tgacgggctt gaatttgagc agcaatccct gacgcagggt ttcgatcgga ttgtcgacca
     2161 ggcctgccac attgaattcg ttgatgatgt ccggatcgct gtaacgctgt gcgacatagc
     2221 cgatcgaaac gatatagatg acgaacagga tcacgtgcgc gacgtagagc tgccagacgc
     2281 gcttggtcag gcgcgtggcg ccgacgacaa aaccgcgttc gatcatcatc cgggcgtaga
     2341 caaaggaggc agtatagcct gagataaaaa caaacagatc cgcggcgtcg ctgaagccat
     2401 agtttcgcgt ggtgatccag ttcacgacgt tgtcgggaat gtgatccaga aaaatcgccc
     2461 agtttgcgac tccgcgaaac aaatcgagcc gcagatcgcg tcctttttca gggagggttg
     2521 cgttgatttt catcgaagtg atgctttcgg gaagatcggc cagcgggcag ggcgcgtcgg
     2581 ccgcgcgacc ggccaagatt gtcacagcgc agcataatga ctataccggt caggacaatg
     2641 taacattcta agctccggaa tcaccggtca cattcctgaa agccgttttt atactatccc
     2701 agccgcttcc accacgcttc catgtcgcat atccaggggg tcctgacatc catgaccgca
     2761 cgtatctcta agcccgccaa aaacgccatg caatcggggg ccgccaagac ccgggaatgg
     2821 cagctcgatt atgagcccga gcagccgcgg gcgatcgagc ctttgatggg ctggaccagt
     2881 tccggcgaca tgaagcagca gctcacgctt cgcttcgaca gcaaggaaga ggccgtggcc
     2941 tattgcgagc gcgaaggcat cccctatcag gtgatcgagc caaaggagcc ggcgcgccgg
     3001 caggcggcct atgccgacaa tttcgcattc cggcgcggcg aaccctggac ccactagggg
     3061 gccccactag ggggcgcatt cgccgaagca tggatccctg gggagtgccg tgggaccgga
     3121 aagttctggg ttgcctaagg cggtggcgca atgacaccat gacgcgagcc ccaatgccca
     3181 gattgcggcg caccataaac cgctgaaggc catgccgctt cattccagta ctgaccccac
     3241 ctcgtccgat ttcgcaggca atgccgaggc catgcggctg ctggtcgcgg aactccgtga
     3301 aaagctcgat caggtcgccg gcggcggcgg cgagacctcg cgtgccaggc acacctcgcg
     3361 cggcaagatg ctggcgcgcc agcgcgtcga tctcctggtc gatcccggca cggcgttcct
     3421 cgaattatcg ccgctggcgg ccgacggcct ctatggcggc gacgtgcatt ccgccagcat
     3481 cgtcaccggt gtggggcgga tatcgggccg cgaatgcgtg atcgtcgcca atgatgcgac
     3541 catcaagggc ggcacctact atccgatgac ggtgaagaag catctgcgcg cgcaggatat
     3601 cgcgcggcag aacaatctgc cttgcgtgta catggtcgat tccggcggtg cgtttctgcc
     3661 gcagcaggac gagattttcc ccgacgaacg gcatttcggc cgcatcttct acaatcaggc
     3721 gcagatgtcg gctgccggca ttccgcaaat tgcgatcgtg atgggctcct gcaccgccgg
     3781 cggcgcctat gtcccggcga tgtcggacga gagcatcatc gtgcgcaatc aaggcacgat
     3841 ctttctcggc ggaccgccgc tggtgaaggc ggccacgggc gaggttgtca cggcggaaga
     3901 gctgggcgga gccgatgtgc attcgaggca gtccggcgtc accgatcact atgcgcagaa
     3961 tgacagccat gcgatcggaa ttgcgcgccg catcgtcgcc accctgaagc agccagcccg
     4021 cgccaacctg aacatgcgcg agccgcgcga gcccttgttt gcgcgagagg aaatctatgg
     4081 cgtggtcccc gccgacggcc gaaaaccgta cgacgtgcgc gacatcatcg cgcgcgtggt
     4141 cgacggttcg gagttcgacg aattcaagaa gctttatggc cagaccctga tctgcggttt
     4201 cgcgcatatc tggggctatc cggtcggcat catcgccaac aatgggatcc tgttcagcga
     4261 gagcgcgctg aagggcgcgc atttcatcga gctgtgctgc cagcgcaata tcccgttggt
     4321 gttcctgcag aacatcaccg gtttcatggt cggcaagaaa tacgaagccg gcggcatcgc
     4381 gcgcgacggc gccaagctgg tgacggcggt ggcgaccgcc gcggtgccga aattcacggt
     4441 cgtgatcggc ggctcctacg gcgccggcaa ttacggcatg tgcggccgcg cctacagccc
     4501 gcggttcctc tggatgtggc cgaacgcccg gatctcggtg atgggcggcg agcaggcggc
     4561 aatggtgctg agccagttgc ggcgcgacaa catcgaagcc aaggacggca gctggtcgat
     4621 cgaagaggaa aacgagtttc gcgcaccgat gcgggcgcag ttcgaggccc agggcaatcc
     4681 gtattacgcc accgcccggc tatgggatga cggcgtgatc gatcccgccg ataccaggct
     4741 ggtgctgggc cttggtcttt cggcagcaag caatgcgccg atcgcgccca ctcgttttgg
     4801 cctgttcagg atgtgacgat ggatcggtcc aaacagtacc ggcgatttcg cactttgctg
     4861 atcgccaatc gcggcgaaat cgcttgccgc gtgatccgca ccgcgaaagc gatgggtttg
     4921 cggacggttg cggtttattc cgaggccgac cgtgacgcga tgcacgtcgc catggccgat
     4981 gaggccgtct tgctgggacc ggcgcgggcg cgcgacagct acctcaacat cgatcgcgtg
     5041 attgccgctg cgcgtcagag cggcgccgaa gccgtgcatc ccggctacgg ttttctgtcc
     5101 gaaagtgccg agttcgcgca agcctgtgcc gacgcgggat taatattcgt cgggccgacc
     5161 gccgacatga tcagggcgat gggctcaaaa tccggctcga agatgctgat ggagaaggcg
     5221 ggcgtgccgc tggtgccggg ctatcacggc gaggcccagg acgaggcgac gctggcgaaa
     5281 tccgccgaca aaatcggctt tccggttctg gtcaaggcct ccgcgggcgg cggcggccgc
     5341 ggcatgcgca ttgtgcgctc ggccggtgaa ctcgcagccg ccatcgtcag cgccaagcgg
     5401 gaagcaaaag ccgccttcgg cgatgatcgc atgctgatcg aaaaattcgt gcaaaatccc
     5461 cgccatatcg aggtgcagat catcggcgac agtcacggca atctgctgtc gctgttcgag
     5521 cgcgaatgca ccctgcagcg gcggcaccag aaggtgatcg aggaagcgcc atcaccgacg
     5581 ctcaatgcca ggcaacgtga gaccgtgtgc gccgccgcgc gcaaggcggc ggccgcggta
     5641 aattatgtcg gcgccggcac catcgaattt gtctcagatg gcaaggacgt gttctttatc
     5701 gagatgaaca cgcggctgca ggtcgaacat cccgtgaccg agctgatcac cggcatcgat
     5761 ctcgtcgaat ggcagctgcg cgtggcgttc ggcgaaaagc tgccgctgat gcaggatgag
     5821 atcaagttaa acggtcacgc catcgaagcg cgagtctacg cggaaaatcc gcacaagaat
     5881 ttcatgccgt cggttggcaa gatacgcacc tggcgcatgc cggagccgtc gaacggcttg
     5941 cgtatcgatg ccgggtatcg cgagggcgat gcggtctcgc cgcattacga cgccatgctg
     6001 gccaaggtga tcgcatgggc gccaaatcgc gacgccgcga tcgaaaggct caatcgcggg
     6061 cttgaggaat cggacgttcg tggcatcgtc agcaacattc cgtttctcgc ggcgctggtg
     6121 acgcatccgg cggtgcgtgc caacgccatc gacaccggct tcattgaacg tgaattgaaa
     6181 aatctcacgc cgccatcggc tgcgcccggc gatctcgagc tttgcgccgc cgtggcggcc
     6241 atcctcggcg aagaagcgaa agctgcgcgc gccgaggcgc attcgccgtg gcgcacggcc
     6301 ggctggatgc cggtcggccg gcggcggcgg gtgtttacgt tccggcatgc gcccgaggcc
     6361 gcgcaggaga tctgcctgca ctacggcagc ggtccggcgg cactgtcgat cggcgagcgt
     6421 gaatttacct ttgcatcgtc accccgggaa gacggcgggt tcgatctcac gctgaacggc
     6481 atgaaatccc acgtcgtcgc cgtgatcgaa ggtcatgaac tgtatttgcg aacccgcaat
     6541 ggccgtttcg acctgcactg ggtcgatccg ttcggcggcg acgacgaaga gcaggtcggc
     6601 gaagacaaga tcgtggcgcc gctgccgggt accgtcgtgg ccttgctggc ggaagtgggc
     6661 gcgaggctgg agaagggcgc acccattctc acgcttgaag tgatgaagat ggagcagacc
     6721 ctgcgcgcgc cattcgccgg cgtgctgagg gcgttaaaat gcaaggtcgg cgatatcgtg
     6781 caggaaggcg tcgaactcgc cgaggtcgaa ccgatagctt cgtgagatca gcatgagcga
     6841 ttccgtacgc atcgtcgaag tcggcccacg cgatggcctg caaaacgaga agacgccggt
     6901 cagcgtcgcc gaccgcattg gctttatcgg ggcgctggtc ggcgccgggc tgcacacggt
     6961 cgaggtcgga gcctttgtat cgccgaaggc gatcccgcag atggtgaatt ccgatcaggt
     7021 gctgcaaggc ttgagccatc acaacgatag cgaattccac gtgctggtgc cgaacgtcaa
     7081 gggttatgaa gccgcgcggg ccgccggctc ccgggtgatc gcggtatttg cctcggcctc
     7141 ggaagggttt tcgcgcgcca acatcaattg ctcggtcgcc gaatccattg atcgcttcag
     7201 gccggtagtc gcgcgtgcca aagccgacgg catcaaggtg cgcggctatg tctcctgcgt
     7261 gctcggttgt ccctttgacg gcgaggtcaa gccgcaggcg gtggtggatg tcgccaggat
     7321 gttgtgggat cttggctgct acgaagtgtc gctcggcgac accatcggca tcggcacgcc
     7381 gctcaaggca cggcatctgt tgcgcgcggt ggccggcgat gtaccgatcg cccatctggc
     7441 gatgcacttt cacgacacct atggccaggc actggccaat ctctatgcgg gcatcgagga
     7501 gggcgcgcgg gtgatcgatt gcgccgccgg cggtctcggc ggctgcccat tcgcacccgg
     7561 cgccaccggc aacgtcgcga ccgaggacgt cgtctatatg ctggagggca tgggcattgc
     7621 gaccggggtc gacatgcaga aattggtggc ggccaccaac gaggtcagca agttgatcgg
     7681 ccgccctcct gtgagccggg tggcggcggc gatcaacgca aagcggggag tggcgaagta
     7741 gccgtgagat gtgaagcgtc atcctgaggt gctcgccgtc ttcggcgagc ctcgaaggat
     7801 gggccacgtt gttgccgtcg cccttcgagg cgcgcgaaga gcgcgcacct cagggtgacg
     7861 gctacctcat cttcccccgt ccggtcccat caccagattc ggcagcgacg tcgaaatctc
     7921 aggcacaaag cacagcacga cgaccgccag catcatcagc agcacgaagg gcagggtgcc
     7981 ccagatcacc tcgctcaagg ggatatccgg cgcgacattg cggatgacga aaatgttcaa
     8041 gccgaccggc gggtggatca ggcccatctc catcacgatg gtcatgacga taccgaacca
     8101 gatgatgtcg aagtgcgccg cgcgcagcgg cggcaggatg attggcgcgg tcatcaggat
     8161 gatcgacacc ggcggcagga aaaagccgag cacgacgacc atgacgagga tcgccgccag
     8221 caattcccat cgcggcaggt gcatcgcgac aatcgactcc gcgaccgatt gcgagatgtg
     8281 caggtagctc atcacatagg aatacaagag cgacatgccg atgatcatca tcagcatggt
     8341 ggactcgcgg atggtcgatt tcaggatcgg cgccaggtcg ctcggccgcc acacgctgta
     8401 gatcagcgcg atcagcacca gcgccagcag tccgccgagc cctgcggtct ccgaaggcgt
     8461 cgcatagccg ccgtagagcg cgatcatcac ccccgtcagc aacagcacga acggcagcac
     8521 ccgcggcaac acgttgaagc gctcggccat ggtgaatttg tcacgcgcga gaatcgccga
     8581 cgtcgcgccg gtcttggcgt aaagcgcgct ggcggcggca tattctttgc gaaaatgaat
     8641 caccgcgtag acgccgaaca acgacaccag cagcaggccg ggcccgatgc cggcgagaaa
     8701 cagccggccc agcgactttt ccgccgcgac cgcgaacagg atcatggtga tcgacggcgg
     8761 cagcaggatg ccgagcgtgc cgccggcggc gatgatgccg gcggcaaatc caccggaata
     8821 accgcgcttg cgcatttccg ggatgccggc ggagccaatg gccgagcagg tggcgggcga
     8881 cgatccggcc atcgcggcga acagcgcgca ggcaaatacg ttggcgatgc cgagcccgcc
     8941 gggcacgcga tgcagccagg cgtggagtgc cgaatagaga tcctggccgg cgcgggattt
     9001 gccgatcgcc gcacccttga ggatgaaaag cgggatcgac aacagcgtga tcgaggccat
     9061 ttcctcgtag acattctgcg tcacggtatc gagcgacgcc gcgggcatgt agatgcccat
     9121 gaacaccacc gcgaccgcac ccagcgcaaa cgcgatcggc attcctgaaa acatcgcgac
     9181 cagcgtcgct atgccgtaac tgattccaat accgagcaca gtcatcgacg tgccggcccg
     9241 cagaggggga tcataatctg cagcaggatc tgcacgcaca ataacgtcat gcccgacgcc
     9301 atcaggccgt agggaatcgc cagcggcggc gaccacatcg agttggaaac ctggccgtcg
     9361 acccacgctt catgggccag cgtccatgat ttccaggcga aaaacccgca aaacagcaaa
     9421 ctggcaacat cgacgagcca taacctgata cgattgacca tcggcgacag cagcccgaca
     9481 aacgcttcga tgccgatgtg gccgcgttgt tgctgcacat aggctgcagt catgaaggtg
     9541 gcgccgacca agagaaacac cgccgcctca tcctgccagt aattcgggct gtgaaacaaa
     9601 gcgcgggtca gcacactgtg gctgagaacg aggcaggctg cgatcagcgc gagcgccgcg
     9661 aagacgacga tgacattgtt gcaaaaagcc agcgcccgtt caaacgccgc cacgagccca
     9721 cggcccgcag cgacgttgga acggtcattt tgatccttga tcggaccatg catcatgcgg
     9781 agacgtcgga cgcgagcttc aacaggttgg cagcggccgc ggtctttgcg ccgtaatcct
     9841 tccacgcggt atcgcgtgcg atgtcgcgcc atttgccgac ggtcgggata tcaagaggga
     9901 agaccttggc gccggccttt tcatagacgt gggcgacttc gatatcgtcg gcctgcgccc
     9961 ccttcttgcc gaacgcttcc aattccgtgc cgacggccac gatgatgtcc tgctgatttt
    10021 tcggcagggc gtcgaaaatc gacttcgaca tcagaaccgg ttccagcatg aaccagtagg
    10081 aggcgccggc gcccgaggtc agggccttcg cgatttcctc gagccggaat gaaatcaggc
    10141 tggtggagga ggtgaggccg gcatcgcagg cgccggtctg cattgccgca tagagttcgt
    10201 tggacggcag cgacagcacc gacgcacccg cggtctgcaa caccatatcc atttcgcgcg
    10261 aaccgccgcg aatcttgagg cccttggcgt cttccggggc gacgaccggg cgcgaacggc
    10321 tggcgacgcc gccggcctgc cagatccagg tcagcatgat gatgcccttg tcggcgagga
    10381 aatccgtcag cgccttgccg accggttcgt tcttccagcg caggccttgg tcataggtgg
    10441 agaccagtcc aggcatcagg ccgatattgg tttccggcac ttcgccgccc gcataggaaa
    10501 gcggaaccag actgatgtcg agcgcgccct tgcgcatcgc ggagaattgc gcgttggtct
    10561 taatcagcga cgagttcgga tagatctcgg cgacgagatc gccgccgctg cgtttggcca
    10621 cctcatcggc gaacacccgc gtcagccgat cgcggaaatc gcccttgtcg accgtgccgc
    10681 cgggaaactg gtgcgatatt ttcaaggtgg tcgcggcatg ggcggttccg gtcccaaaac
    10741 gcaggatggc gggcgcggca agcgcggatg cgaggacata gcggcgtgtc agcatttgta
    10801 tctcccgggt tgtactctac ttagaatgct cgagccggcg ttgagggata ttagcccgtc
    10861 ttcatcagaa gtttcactag tctgatagtt cggggctgaa ttcggcaggg gggcgccggc
    10921 gctgttgcgg cgcacacttg cgggaaagag ttcggcagaa agaagaccaa ccgccaactt
    10981 tagcgagaga aatcataccg aagcaaaagc acgcttcaaa cgctttttat gcgagaaatt
    11041 tctcaagcgg agaaattttt caggcaaagg gtaacaaacc gtgttgtggg atcgttgtca
    11101 tgtggcggca tcagtcgctt tcaaaccagt tttcaaagga atgatcacat atgaccatca
    11161 gcacccgctt tgcgctcgga atttcggccg ccactctttc gctcagcctg gcgctcgccc
    11221 cggtcgcctt cgcccaggac aagatgggca aggacgacgg catgaagaaa gacaccatgt
    11281 ccaaggacac gatgaagaag gacacgatgt ccaaggacgc catgaaaaag gacgacggca
    11341 tgaagaagga cggcatgatg aagaaagatg acggaatgaa gaagtaagcg tcgccgcttg
    11401 gtttcccggc aggctcgcct cgatcagagg cgggcctgtg atgttcgggg gcgtcgcgcc
    11461 gcccgaaatt tgccagtcat cttatttccg cttcttgcgg gccgcatagc gcgcgtcgcg
    11521 cgcggccttg cgttcggcct cgagcgccac ttcccgggcg gcggcctctt ccttttcgcg
    11581 tgccagttgt gctgtggcct gcgccgcagc ctcggcctcg cgggctatct tctcggcctt
    11641 ggcggcctcg cgcgccaatc gggccttggc tcgctcggcg gcctgggctt cgcgctcggc
    11701 ctgccgctgc ctgaccgcgg gatcgtcgag acccggcttg gctcggaact tgttcaggat
    11761 gttctgcctg gcttcctgtg ccgccttctg ccggtctgcg aagctgggtt ccttgaatcc
    11821 actcattgaa cgggccttgt cgctttcatt gtttggtgtg agacgatcga tttgccggtc
    11881 gcgtcggcgc cgcgctcggg gcgtttccac gatcgggagc ggtgtacagc cgcacgagcc
    11941 cggaagccat caattaatcg gttaaccacc cccgtccgaa gcaagaaaaa tgcggttcgc
    12001 ggccccattt caacggcccc gtcgcgtgat ttcgccgatt ttgctgccgc cccagcgcga
    12061 caccggggaa gatgcgcgca ctatcaccgg atcagcttca tgatcatgac aagcagccag
    12121 agatcgccgg cggctttcgg gcctcgcagt tgattgagac cggcggcgcc aaggtacatg
    12181 ccgaacgccc cgaggccgat gaccacagcc gcttgctccg acgcgccctg aacgagcgcg
    12241 agaaaataac agaacgcaaa caggaaagga gagaggatga gcaaggctct gacggcctgc
    12301 gccgtcggtt ccgacctatc cggattttct tcgcgcatcc ggttcatttc gcggtgcgat
    12361 catccggcgg atgaggtcgc tcctttgctg tgtcgcctgc gccgccagtt tgtccctggc
    12421 gcgctcgtcg atcagcaaat tgatcaacgc cagccgcctc ggctcgtcga ccgcttcggc
    12481 cagcaatttt cggtagcgcg gatagtcgaa ttccgaatcc tgctgattca taggcgtgcc
    12541 cccgcacgga ttcccgaacg aagtgtttga ttccccgaac gaagtgtcag ggaatgccaa
    12601 cggccgcgca atgcgattta agttttcccg gtgctctatc caccgatcaa tgcttgctga
    12661 agggccaaag ggagtt
//
LOCUS       DASWGM010000365        15251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2659722,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000365 DASWGM010000000
VERSION     DASWGM010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2659722"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..2041)
                     /locus_tag="VGA15_10620"
     CDS             complement(104..2041)
                     /locus_tag="VGA15_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588183.1"
                     /translation="MSNGPNGLDQLTAGAKPPRAASSFGKAQTPVLSISGLATSFIRD
                     GQWIPVVRNVSLDVAAKETVAIVGESGSGKSVTALSIMRLIPRESGRIQGSIKLAGRD
                     LLTLPERTMQDVRGNEVAMIFQEPMTSLNPVLTIGFQIAEALIYHRGMSRSAAEAETI
                     RLLEKVRIPAAKSRFHEHPHRFSGGMRQRVMIAMALACKPKLLIADEPTTALDVTIQA
                     QILELLKVLQEEEDMSILFITHDMGVVAEIADRTVVMYDGQAVETNSTTQIFAAPSHP
                     YTRALLSAVPRLGSMEGRARPMRFPIVDKVTGTSDEPTETPDTAATAERPVLEVANLT
                     TRFAIRSGLFGKVSGRVHAVENVSFSLHAGETLALVGESGCGKSTTGRSILKLIEPDS
                     GAILVDGADVLGMGTRALRELRKRMQIVFQDPFASLNPRMSVGSAIAAPLYAHGLASS
                     SQARDRVADLLVRVGLTADMASRFPHEFSGGQRQRICIARALALGPKLIIADEAVSAL
                     DVSVKAQVVNLMLDLQASMGLAYLFISHDIAVVERMSHRVAVMYLGEIVEIGPRAAVF
                     GNPQHPYTKKLMAAVPIPDPARRGEKRGVSNDEIRSPVRAPDYQPPVRQYREVTPGHV
                     VQLWGEEWPVCVPKTLSELMT"
     gene            complement(2066..2992)
                     /locus_tag="VGA15_10625"
     CDS             complement(2066..2992)
                     /locus_tag="VGA15_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEX9588184.1"
                     /translation="MTFLYKANATRGMEWARFFAARAPDLPFRLWPDVGDPAQVRYLA
                     VWAPPENIAATFRNLELVFSVGAGVDQFDFSQLPPHVPLIRMLEPGIAESMVEYVTIA
                     VLALHRDLVQFIAQQREQTWREIRITQAGKRRVGVMGLGLLGQAVLERLKLFGFPLAG
                     WNRSPRTIQDVSCYAGGQALSDFLAQADILVCLLPLTSETRGILNTSLFEALPCGAQL
                     VNVGRGGHLVEADLIEALERGLLSAAILDVAEHEPLPAGHPFWSHPRILLTPHIASMT
                     TPEAAVEFVLETIGRHRRGETLPGLVDRERGY"
     gene            complement(3018..4346)
                     /locus_tag="VGA15_10630"
     CDS             complement(3018..4346)
                     /locus_tag="VGA15_10630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9588185.1"
                     /translation="MSPPLSRIASDERLPAQVDVVVIGGGVIGVSAAYHLAKKGHSVA
                     LIEKGHVGAEQSSRNWGWCRQQGRARAEIPLAREALRLWEDMQNDAGVDAGFRRTGVL
                     FLTKNPQELAAWEKWAEIAREQQIHSSVLSPAEIAERMPGNADTWVGGLHTPSDGRAE
                     PSVAVPALAVAARKHGVTIHQGCAARGLETQGGQVSAVVTEKGTIRAQSVLLAGGAWS
                     SLFCRRHGIELPIGLVNATACRTTPAPEITTGALGTDFYCIRRRLDGGFTLALRNRGT
                     VEVSPDLFRYARTFWPTYQHRRKGLKLSFGKSFFDQIMRGTNWSLDKPSPFEAERVRD
                     PAPDMSLVNSALAALIKSNPELKGIEVAEAWGGTIDCTPDTIPVISPVDSLPGFFLAS
                     GFSGHGFGIGPAAGKLAADIVTGDAPLVDPAAYSHVRLIDGRPLAPVTLF"
     gene            complement(4390..5265)
                     /locus_tag="VGA15_10635"
     CDS             complement(4390..5265)
                     /locus_tag="VGA15_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588186.1"
                     /translation="MSTAALQTVQLAPGQAAWQRFRRHRLALAGAVTIVVLVLGAAFG
                     PYLLPFDDTFIDIMKRFAPPLSGAHILGTDELGRDVLARLMMGGRVSLSIGIVAMTIA
                     MAVGILVGAFAGFYGGVLGAVLMRLVDAVLCFPAIFLLLALAALTEPGLMTTTLLIAA
                     TAWMSVARVVEAQVRSLREREFAIAAVAFGSSSLRIMFRELVPNAMAPIVVAATLNVA
                     KAILLESYVSYLGYGIQPPAASWGNMLNNAQIYLTSAPWLAIAPGVAITLAVTSFNFL
                     GDGLRDALDPRLNIP"
     gene            complement(5269..6219)
                     /locus_tag="VGA15_10640"
     CDS             complement(5269..6219)
                     /locus_tag="VGA15_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588187.1"
                     /translation="MARYVANRLMQAIMLLVIVSALGFAILHLAPGGPLSQFAASSQM
                     TQEDLERVTRQLGLDRPLPIQYLDWFGRMLRGDWGRSYRDGEAVLSVIGSHLGATLEL
                     MATATAIAILLGCWIGVLGAVRRYSLFDSLATVGAMIALSIPTFWFGLVTIYLFSVNL
                     GWLPSGNRQTIGDGSLLDLLHHLIAPAMVLALVETAIWGRFMRSSMLEVINQDYIRTA
                     RAKGMPEWRILTLHALRNALLPMITVAGLQFPTLLGGALVTETVFTWPGMGRLFLDSI
                     GYRDYPVVMGILMFSAIMVLIGSLLADILYAVVDPRIRVG"
     gene            complement(6247..7944)
                     /locus_tag="VGA15_10645"
     CDS             complement(6247..7944)
                     /locus_tag="VGA15_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9588188.1"
                     /translation="MADETGKFDIPALGNLTPGIPNRRQFMLLGASAAAGMTVPASAF
                     AQTERPTNPPDKPRGQVIAALSQEPTVFHPLMPGIEVDQGVWWQVFSPLWFIDPDGNF
                     VPDLAREVPTIENGGLSADGLTWKIKLRSDAKWHDGTAFTAEDVKFSLELINNPDFRA
                     RNRVGHNLIKDIKVDAPDEIHWRMEAPYSPYMSILSLTCMVPKHILEKVSDPNSSPFH
                     NAPVGTGPFRWGERVPGDHILLNAHAAYHGKGPYLERVVFKYIPDLTVLYTQFRTGQV
                     DYTGLQGILPNFVQEAKTLRGCKIFVSSTASVEHVAPNLEFGPFVDRSVREALYLAIN
                     KKAIIDALNYGLPTQTESFVPQQAWSFQQGLPQHSYDPAKANALLDAAGWVRGAGGIR
                     EKGGVKLEFTNSTTSGNAVREQTQQLLIQDWRAIGAAMRVNNMPAAVIWGDFWQQSKF
                     NSVLVSVNFMLGSDPDVTPRFGSGAIPAKGGRGFNTYQYQSPEADRLLAEGAKQFDLA
                     QRKVIYGDLQKLIRKDLAVLPLFQGCIAEGVKDGLQGFRHNINASSNCWNIREWYWT"
     gene            8177..8809
                     /locus_tag="VGA15_10650"
     CDS             8177..8809
                     /locus_tag="VGA15_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9588189.1"
                     /translation="MGGGSGASVPKNAQTIENDEAVDQRLGETVRLLRQRAGFSIQDV
                     ANRTGLSTGMISQLERARAMPSVRTLRLLSIALDVPISYFFEASDAGEPQRYIVRKND
                     RRLLRLTASGVVKEALTPAEKGELELYELTLNPGGSSGTDFFQHTGEKAGYILSGSLR
                     LWLDHEAHVLEAGDSFRFPSIVPHMFDNPTQQVARVIWVTTLRRTDPPAS"
     gene            8998..9474
                     /locus_tag="VGA15_10655"
                     /pseudo
     CDS             8998..9474
                     /locus_tag="VGA15_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273253.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(9477..9764)
                     /locus_tag="VGA15_10660"
                     /pseudo
     CDS             complement(9477..9764)
                     /locus_tag="VGA15_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316945.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(9829..10782)
                     /locus_tag="VGA15_10665"
     CDS             complement(9829..10782)
                     /locus_tag="VGA15_10665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX9588190.1"
                     /translation="MRRLLVAEGNASAGRRRIAESAGATPAESYADVLRAIAQDAKVD
                     ICTPADEGAMVPAPLDSYDGVAITGSSLNIYQREVASLRQIDFVREVFARAIPMFGSC
                     WGLQLAAVAAGGEVTLNPRGREVAFARKIILTDAGRSHPMHRRRSPVFDAPAIHSDVV
                     TRLPQGAIITAWNAMSEVQAAEIRLGGGVFWGVQYHPEYRLHDVAAVVRRYGETLVVE
                     GFFKDLSELESYAADLTTLETDQKRRDIAWRLALARIFSTKGCGCQNYRTGSPARCAD
                     EGGLTHDLQRRLMSEEISQIWVVLGKMIRLSGHLQRLADIV"
     gene            complement(10786..11958)
                     /locus_tag="VGA15_10670"
     CDS             complement(10786..11958)
                     /locus_tag="VGA15_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX9588191.1"
                     /translation="MATNIAGAWNFPTRVITGAGRIAELPETCRAVDIAKPLLVTDKG
                     LANTALIAGVVKHLCDAGIEVRIFADVKSNPTETNLNAGIAAFRAGGHDGVIAVGGGS
                     ALDVGKCVAFMVPQSRPVWDFEDVGDWWTRANSKGIAPIIAVPTTAGTGSEVGRAGVI
                     TREETHEKKIIFHPLMMPKVAIEDPELAAGLPPFLTMATGMDALAHCFEAYCVDAFHP
                     LADGVALEGLRIVDTYLPRAVDNGGDLEARAYMFAAASMGATAFQKGLGAIHSVSHPV
                     GARYDTHHGLTNGVIFPYVLVCNKTAIADKIPHIARTLDLPGRDFSAVLDWILALRKK
                     LGVPHTLAELGVREVDVKAIAADAAKDPTAAANPRRLTEAEFEQLTLAAIRGDLGG"
     gene            complement(12012..13379)
                     /locus_tag="VGA15_10675"
     CDS             complement(12012..13379)
                     /locus_tag="VGA15_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX9588192.1"
                     /translation="MASRDVKTAEDARALVDARGLSHVKLGVVDLDGVIRGKYIARDK
                     FFAALEAGFNFCDVIFGWDSQDQLYDKSRFTGWHTAFPDATARVIPNSCRDVPTEGDM
                     LFFLGEFEGAAADLCPRNLLGRVVERAAKLGFTASAAAEFEFFVFDETPHSVREKGYR
                     SLKNLTPGWFGYSMLRSSVESDFHHGLLKLCDDMDMPLEGLHTETGPGVLEAAIKYTD
                     VMAAADRAVIFKTFSKVLAERQGKMLTFMAKWSNSYPGQSGHLHLSLRDLEGKPAFFA
                     ADRPGNMSDTMRWFVGGQQALLPEVLAMVASTVNSYSRLIPGFWAPTDATWGIENRTC
                     ALRVIPGAPSAQRVEYRIAAADINPYLALAAALGSGLWGIQHRIEPGSPIVGNAYDIS
                     HPPERSLPRTLFEAAERLAASSAARSLFGDVFVDHYAMTRCWEQREFQRAITDWELAR
                     YFEII"
     gene            complement(13457..14800)
                     /locus_tag="VGA15_10680"
     CDS             complement(13457..14800)
                     /locus_tag="VGA15_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9588193.1"
                     /translation="MPQEKEILALNAFDQKNASAMSTDLREAVARRLRSFGSASVLFY
                     QEPLRMERAEGVWMYDADGRRYLDLYNNVPSVGHSHPRVVEAICKQAGLLNTHTRYLN
                     DVVDLYAERLLATFPDAIDHLVLTCTGSEANDLALRIAQAATGGTGFIVTETAYHGNT
                     AAVTGVSPSSLPGQALPPHVRAIPAPEMFRCAGSDPGQRFAASVTTAVADLKRNGIRF
                     AGLLVDTIFSSDGVYADPPGFLTAAVEAARAHGGIFIADEVQPGFGRTGASLWGFARH
                     DVVPDIVTMGKPMGNGFPMGGIATRAALLDRFTAEAKYFNTFGGNPVAAAAGLAVLDT
                     IRDEGLTENAFRVGDYLMDGLREIGNRHIQIGDVRGAGLFIGLELVNDRETKAPAPEI
                     ASHLINTLRQRGILLGAAGPYGNTLKIRPPLCFTKDNADMFITACDEVLHQNFPA"
BASE COUNT         3063 a         4765 c         4712 g         2711 t
ORIGIN      
        1 cgcgttctcg gcttcaagcc gcaacttcga cttgaatggc gaggccagga tggccagaac
       61 gaaacaaagc agcccgatca ttccgccagc ttaggcgatt tcttcacgtc atcaactcgg
      121 ataaggtttt cggtacacac actggccatt cttcgcccca aagctggacg acgtggcccg
      181 gcgtgacctc gcggtactgg cggaccggcg gctggtagtc gggggcgcgc acgggactcc
      241 tgatttcgtc gttcgatacg ccgcgcttct cgccgcggcg cgcaggatca gggatcggta
      301 cggcagccat gagcttcttc gtatagggat gctgcggatt cccgaagacc gccgcgcggg
      361 gaccgatctc gacaatttcg ccgagataca tgaccgcgac gcgatggctc atccgctcga
      421 cgaccgcgat gtcatgggat atgaagagat aagcaaggcc catactcgcc tgaagatcga
      481 gcatcagatt gacgacctgt gccttgaccg agacgtcgag agccgaaacc gcctcgtcgg
      541 caatgatcag cttgggacca agcgcaagcg cgcgggcgat gcagatgcgc tggcgctgac
      601 cgcccgagaa ctcgtgcgga aagcgcgacg ccatgtctgc cgtcagcccg acgcgaacca
      661 gcagatcggc aaccctgtcg cgcgcttgcg atgaagaggc cagcccgtgg gcatagagcg
      721 gcgcggcgat cgccgagccg accgacatgc gtggattgag gcttgcgaac ggatcctgga
      781 agacgatctg catgcgcttg cgcaactcgc gcagggcacg ggtacccatg ccaagaacat
      841 ccgcgccatc gaccagaatg gcgccgctgt ccggctcgat cagcttgaga atggatcggc
      901 ctgttgtcga cttgccgcaa cccgattcac caaccagcgc cagcgtttcc ccggcgtgca
      961 ggctgaaaga tacgttctcc acggcgtgaa cgcggcctga gacctttccg aacaggccgg
     1021 aacggatcgc aaagcgcgtt gtcaggttgg cgacctcgag caccggccgc tcggcggtgg
     1081 ccgccgtgtc cggcgtctcc gtcggctcgt ctgacgtccc ggtcactttg tcgacgatcg
     1141 ggaaacgcat cggccgcgcc cgtccctcca tggagccgag acgtggcacg gccgagagaa
     1201 gcgcacgggt ataaggatgc gaaggagcgg cgaagatctg tgtggtgctg ttggtctcca
     1261 ccgcctgtcc gtcatacatg accacggtgc ggtccgcgat ttcggcaacg actcccatgt
     1321 cgtgcgtgat gaagaggatc gacatatcct cttcctcctg cagaaccttg aggagctcca
     1381 ggatctgcgc ctggatcgtg acgtcgagcg cggtcgtcgg ctcgtcggcg atgagcagct
     1441 tcggcttgca ggccagcgcc atggcgatca tcacgcgctg gcgcatgccg ccggagaagc
     1501 gatgaggatg ttcgtggaaa cgcgacttcg ccgccggaat gcggaccttc tcaagcaggc
     1561 gaatggtctc ggcctcggct gccgagcgcg acatgccccg atggtagatc agcgcctcgg
     1621 cgatctggaa gccgatggtc agcaccgggt tgaggctggt catcggctcc tggaagatca
     1681 tggcaacttc gttgccgcgc acatcctgca tcgtccgctc gggcagcgtc aggagatcac
     1741 gacccgcgag cttgatgcta ccctggatgc ggccgctctc tcgcggaatg agccgcatga
     1801 tggagagagc agtcacgctc ttgcccgagc ccgactcccc gacgatcgcc accgtctctt
     1861 tcgcggcaac gtctaacgac acgttccgga cgacgggaat ccattgccca tcgcgaataa
     1921 acgaggtggc aaggccggaa atcgacagga ccggggtttg cgcttttccg aacgaactcg
     1981 ctgcccgggg cggcttcgcg ccggcagtga gttggtcaag tccgttcggg ccattactca
     2041 tcctgaagct ttcaaaagcg ctgtttcaat aaccgcgttc acgatcgaca aggcccggca
     2101 aggtttcgcc gcggcggtga cgaccgatgg tttcgagtac gaattcaacc gccgcttccg
     2161 gcgtcgtcat gcttgcgata tgcggtgtaa ggagaatgcg tggatggctc cagaacggat
     2221 ggcccgcggg cagcggctcg tgttcggcga cgtcgagaat ggcggctgac aacaggccgc
     2281 gctccagagc ctcgatgagg tctgcctcga ccagatgccc gccccgcccg acattgacga
     2341 gttgagcacc acacggcagt gcctcgaaga gacttgtgtt caggatgccg cgggtttcgc
     2401 tggtcagcgg tagcaggcag acaaggatgt cggcctgggc gagaaaatcc gataaggcct
     2461 gcccgcccgc ataacagctc acatcttgaa tggtgcgtgg cgaacggttc cagcccgcaa
     2521 gcggaaagcc gaatagtttg agccgctcaa gcaccgcctg accgagcaag ccgaggccca
     2581 tgacgccaac tcgccgtttc ccggcctgcg tgatccggat ctcgcgccag gtctgctccc
     2641 gctgctgggc gatgaactgc acgagatcac gatgcagtgc gagcacggcg atggtgacat
     2701 attcaaccat gctctccgcg atgccgggct ccagcatgcg gatgagcgga acatggggcg
     2761 ggagctgcga gaaatcgaat tgatcgaccc ccgctccgac cgagaagacg agctcgagat
     2821 tgcggaaggt tgctgcgatg ttttccggcg gcgcccacac cgccaggtag cgcacctgcg
     2881 cggggtcgcc gacatctggc caaagccgga acggtaggtc gggggcgcgc gccgcaaaga
     2941 agcgggccca ttccatgccg cgggtcgcgt tcgccttgta gaggaatgtc atgccgcggc
     3001 cccaatccga caccgcgcta gaacagggtg accggcgcaa gtggccggcc atctatcagg
     3061 cgcacgtggc tgtacgccgc cggatcgacc agcggtgcat cgcccgtcac gatgtcggcg
     3121 gcgagcttgc cggccgccgg cccgatgccg aatccgtgac cgctaaagcc gctcgcgagg
     3181 aaaaagcccg gcagggaatc gacgggagaa atcaccggaa tggtgtctgg cgtacaatcg
     3241 attgtgcctc cccaggcctc cgcgacttcg atgcccttca actcgggatt tgacttgatc
     3301 agcgccgcga gcgccgaatt gacaagcgac atgtccggcg cgggatcgcg cacgcgctca
     3361 gcctcgaacg gcgaaggctt atcgaggctc cagttcgtac ctcgcatgat ctgatcgaag
     3421 aacgactttc cgaacgacag cttcagcccc ttccggcggt gctggtaggt cggccagaag
     3481 gtccgcgcat agcgaaaaag atccggcgac acttccaccg tgccccggtt gcgcagcgcg
     3541 agcgtgaagc cgccatcgag gcggcggcga atgcaataga agtcggtgcc gagagcgccg
     3601 gtggtgattt ccggcgccgg cgtggtgcgg catgcggttg cgttgacgag gccgatcggc
     3661 agctcgatgc cgtggcgccg gcagaacagc gacgaccacg cacctcccgc aagcagcacg
     3721 gactgcgcgc gaatggtccc cttctcggtg acgaccgcgc tcacttgtcc gccttgggtc
     3781 tcgagcccac gcgcggcgca gccctgatga atggtgacgc catgcttgcg cgcagcgacc
     3841 gcgagcgcag gcaccgccac cgaaggctcg gcgcgcccgt cactcggcgt gtgcaaaccg
     3901 ccgacccaag tatcggcatt gccgggcatg cgctcagcga tctcggccgg cgacaagacc
     3961 gacgaatgaa tctgctgttc gcgcgcaatc tcagcccatt tttcccatgc ggccagttcc
     4021 tgcgggttct tggtcaggaa caagacaccg gtacgccgaa agccggcatc aacgccggcg
     4081 tcgttctgca tgtcctccca caggcgcagc gcctcgcggg caagcgggat ctcggcgcgc
     4141 gcgcggccct gctggcggca ccagccccag ttgcggctcg actgctccgc gccgacatgt
     4201 cctttctcga tcagcgcgac ggagtgtcct ttcttcgcca gatgataggc cgccgaaacg
     4261 ccgatcacgc caccgccgat gacgacaaca tcaacctgcg ccggcaagcg ctcatcgctt
     4321 gctatacggc tgagcggcgg ggacatgaga ctctcctgac aataaggttg atcgaagccg
     4381 gttggctctt catgggatgt tcaagcgcgg atcgagggcg tcgcgcagtc cgtcgccgag
     4441 gaagttgaag ctggtcacgg caagtgtgat ggcaacgccc ggcgcaatcg caagccaggg
     4501 cgcactcgtc agatagattt gggcattgtt gagcatgttg ccccagctcg cggccggcgg
     4561 ttgaatgccg tagccgaggt agctgacata ggattcgagc aggatcgcct tggcgacgtt
     4621 gagcgtggcc gcaaccacga tcggcgccat cgcattgggc acgagttcac ggaacatgat
     4681 ccgcagactc gacgaaccga aggccacggc ggcgatcgcg aactcgcgtt cgcgaagcga
     4741 acggacctgc gcttccacaa cgcgggcgac gctcatccac gcggtggccg cgataagcaa
     4801 ggtggtggtc atcaggcccg gctcggtgag cgccgcgagc gccagcagga gaaagatcgc
     4861 cgggaagcac agcacggcat cgacgagccg cattagcacc gcgccgagga cgccgccgta
     4921 gaagcccgcg aaagcgccga caaggatgcc gaccgccatg gcgatggtca tggcgacgat
     4981 gccgatggag agcgacacgc gcccacccat catcaaccgc gccagcacgt cgcggccgag
     5041 ctcgtcggtg ccaagtatat gcgctcccga caacggcgga gcgaaccgct tcatgatatc
     5101 gataaaggtg tcgtcgaatg gcaaaagata agggccaaac gccgcaccca ggacaaggac
     5161 gacaatcgtc acagcacccg ccaaagcgag ccggtgacgg cggaagcgct gccaggcggc
     5221 ttgaccggga gcaagttgga cggtctgcaa ggccgcggtg ctcatcgcct atcccacccg
     5281 gatccgcgga tcgacgacgg cataaaggat gtcggcgaga agtgaaccga tgagcaccat
     5341 gatggccgaa aacatcagga tgcccatcac caccggataa tcgcgatagc cgatggaatc
     5401 gaggaacaac cggcccatgc cgggccaggt gaacaccgtc tcggtgacca gcgcgcctcc
     5461 gagcaacgtt ggaaactgaa ggcccgcaac ggtgatcatc ggcagcagcg cattgcgcaa
     5521 tgcgtgcagc gtcaggatgc gccattccgg catccccttg gcgcgcgccg tgcggatgta
     5581 atcctggttg atgacctcga gcatggaaga gcgcatgaag cgtccccaga tggcggtctc
     5641 gacaagtgcc agcaccatgg ctggtgcgat cagatgatgc agaagatcga gcagtgagcc
     5701 atcaccgatc gtctgccggt tgccggacgg caaccagccg aggttcaccg aaaagagata
     5761 gatcgtgacg agcccgaacc agaaagtcgg aatggagagc gcgatcatgg cgccgacagt
     5821 tgccaacgaa tcgaacaatg aatagcggcg cacggcgcca agcacgccga tccagcagcc
     5881 gagcagaatg gcgatggctg tcgcggtcgc catcagttcg agcgtggcgc cgagatgaga
     5941 gccgatgacg gacagcacgg cctcaccgtc ccgataagag cgaccccagt cgcctctgag
     6001 catgcggccg aaccagtcta gatattggat cggaagcgga cgatcgaggc ccaactgcct
     6061 ggtgacgcgc tcgaggtcct cctgcgtcat ttgcgacgag gccgcgaatt gcgagagcgg
     6121 gccgccaggc gccagatgga ggatcgcaaa gccgagcgcc gagacgatca ccaacaacat
     6181 gatcgcctgc atcaggcgat tggcgacgta acgggccatc tcaggcaatc cccctgatcg
     6241 gacgcgtcag gtccagtacc attcgcggat gttccaacag ttggacgagg cgttgatgtt
     6301 gtgccggaag ccttgcaatc cgtccttgac gccctcggcg atgcagccct ggaacagcgg
     6361 cagcactgcg aggtccttgc ggatgagctt ctgaaggtcg ccgtagatca ccttgcgctg
     6421 tgcaagatcg aactgcttgg cgccctcggc gagcagccgg tcggcctccg gactctggta
     6481 ttgatacgtg ttgaaaccgc gtccaccttt ggcgggaata gcacccgaac cgaagcgtgg
     6541 cgtcacgtcc ggatcgctgc ccagcatgaa gttcacggac accaggaccg agttgaactt
     6601 cgactgctgc cagaagtcgc cccaaattac cgcagccggc atgttgttca cgcgcatcgc
     6661 ggcgccgatc gcgcgccagt cctgaatcag taactgctgc gtctgctcgc gcacggcgtt
     6721 gccggacgtg gtcgaattgg tgaattcgag ctttacgccg cccttctcgc ggatgccgcc
     6781 tgcgccgcgg acccatccgg ccgcatcgag cagagcattt gccttcgcgg gatcgtagct
     6841 gtgctgcggc aggccctgct ggaacgacca ggcctgctgc ggcacgaagc tctcggtctg
     6901 cgtcggcaaa ccgtaattga gcgcatcgat gatcgctttc ttgttgatcg caagataaag
     6961 cgcctcacgc accgagcggt cgacgaatgg gccgaattcc aggttcggcg cgacatgctc
     7021 caccgaggcc gtcgaggaca cgaaaatctt gcagccgcgc agcgttttcg cctcctgcac
     7081 aaagttcggc aggattccct gcaagcccgt gtaatcgacc tgacccgtgc ggaactgcgt
     7141 gtacaggacg gtcaagtccg ggatgtattt gaagacgacg cgctccagat aaggcccttt
     7201 gccgtggtag gcggcatggg cgttcaacaa aatgtggtcg ccaggcacgc gctcgcccca
     7261 gcggaacggt cctgtaccga cgggcgcgtt gtggaacggc gagctgttcg gatccgagac
     7321 cttctccagg atgtgtttgg gcaccatgca ggtcagcgac aggatcgaca tgtagggcga
     7381 atagggcgcc tccattcgcc aatgtatctc gtcgggcgca tcgaccttga tgtccttgat
     7441 caggttatgg ccgacgcggt tgcgcgcgcg gaaatcgggg ttgttgatca gctcgagaga
     7501 gaacttcacg tcctccgccg tgaacgctgt gccatcatgc cacttcgcgt cgctgcgcag
     7561 cttgatcttc caggtcagcc cgtcggccga cagcccgccg ttctcgatgg tcgggacttc
     7621 gcgggccagg tcaggaacga agttgccatc aggatcgata aaccacagcg gcgagaagac
     7681 ttgccaccaa acgccctgat cgacctcgat accgggcatg agcggatgaa agaccgtcgg
     7741 ctcctgcgac agagccgcga tcacctgccc gcgtggcttg tcgggcggat tggtcggacg
     7801 ttccgtctga gcaaatgcgg aagctggaac ggtcattccc gccgcagcgc ttgcgccgag
     7861 cagcatgaac tggcggcggt tcggtatgcc cggggtcagg ttgcccaacg ccggaatgtc
     7921 gaatttgcca gtttcgtccg ccatgagccc tctacgtttt cgcgcgcggc tatcccccgc
     7981 gccccgttct cgaagatccg gcaacggaga gattgactga gcttcagcac ctctaactat
     8041 ttgtatgtaa atttcatcac agctaaataa acgagtcaat cctgttgatg gtatgactcg
     8101 attgttcaat gaaattaatc tccgcttggt gatgtgcgcg aaacggttgg acggcgagac
     8161 gatacgggag aacgacatgg gtggcggcag cggcgcgagc gttccgaaga acgcccaaac
     8221 gatcgaaaac gatgaggcgg tcgaccagcg tcttggcgag accgtgcggc tcctgcgcca
     8281 gcgtgcaggt ttctcgatcc aggacgtcgc caaccgtacc ggcctgtcca ccggcatgat
     8341 cagccagctc gaacgcgcgc gcgctatgcc ttccgtgcgc acgctgcgtc tcctcagcat
     8401 cgctctcgac gtgccgatct cctatttctt cgaagcgagt gacgcaggcg aacctcagcg
     8461 ctacatcgtg cggaagaatg atcgccggct tcttcggctc accgccagcg gcgtcgtcaa
     8521 ggaagcgctc acgccggccg agaagggcga gcttgaactc tatgaactca cgctcaatcc
     8581 tggcggctcg tcgggcaccg acttcttcca gcacacgggt gaaaaggcgg gctacattct
     8641 gtcgggcagc ctacgtctgt ggctagacca cgaggcccat gtcctggagg cgggcgatag
     8701 tttccgcttc ccgagcatcg tgccacacat gttcgacaac ccgacgcagc aggtggcgcg
     8761 tgtcatctgg gtgactacgc tgcgccgaac cgatccgccg gctagttgaa ccgcggtgtg
     8821 gcacgcttac gtcgcaggat cgcgtcagcg tgcaatcgtc gctctgacgc agaattggtg
     8881 caaccacggc ttcacgcggc accgatcggt ctcgcctgaa gcaggtcgac cttcgctctt
     8941 cgctacattt ggcgcttcac aagtttcaac ttcatgattt gacctttggt ctggccgctt
     9001 gacgtcgcgg tcggacaacg gatccgcgat ttgccgcgaa cccgcgccat gtcgctcgag
     9061 acggttgccg cccgcaccga tttatcgatt ggggccagat cgagcgcggc ctgtcctcgc
     9121 cgtcgctacg ggtgctggcg actctcgctg acgtcatggg cgtgggtatc gcagggtttt
     9181 ttccgcgaca aggagaacgc caacgccacg ccaagcggca ttgtcacccg caagcgccag
     9241 cgcgccaatg gagcccggcg gaagcactgg tgacgaattc tatacgcatg acggcgagga
     9301 agccggcctc gtgctcgagg gcgacatgat gctcaccgtc gatgccgaaa gctggacact
     9361 caagtacggc gacagcttcc gcttcgccag ccgctgccca catcggttca gcaatccgtc
     9421 cagtgacacc agggccgtcg tgctctggtc aactgcgtct cggccgcgtc ctgagatcag
     9481 ccgaaacgat tctcgtatct ggccagtgcg agttccgccg tgtcgatgtc gggcttgcgg
     9541 cccgacagta gatccgcaag cacccgcccc gaaccgcacg ccatggtcca gccaagcgta
     9601 ccatgaccgg tgttgagatg aaggttggcg tagagtgtcg cgccgatcac cggcggaccg
     9661 tcgggagtca tcggccgcaa gccgctccag aaggtggcgt ccgccagcct gccgccgcgc
     9721 gggaacagat cggtcagaga atgatcgagc gttgcctgcc gcgccaggcc cagccgggca
     9781 attttccggg cttgtcgaga acaccgcgcc gttccttggt gcggtgaact agacgatatc
     9841 tgcgagccgc tgtaggtgcc cggagagcct tatcatcttt cccaagacga cccagatctg
     9901 cgatatttcc tcactcatca gacgccgctg caagtcatgc gtcaggccgc cctcatctgc
     9961 gcacctggct ggtgatccag ttcgataatt ctgacaaccg catcccttcg tcgagaatat
    10021 cctcgctaaa gccagccgcc atgcgatgtc ccgtcgcttc tggtcggtct caagagtagt
    10081 cagatccgca gcgtaagatt caagctccga gagatcctta aagaagccct cgacgaccaa
    10141 cgtctctcca tagcgtcgga cgacggcggc gacgtcgtgc aaccgatatt cggggtgata
    10201 ctgcacgccc cagaacacac cgccacccaa acggatttcg gcagcctgga cctcgctcat
    10261 cgcgttccag gcagtgatga ttgctccttg cggcagccgc gtcaccacgt cgctgtggat
    10321 cgcgggcgca tcgaaaacgg gcgagcgcct gcgatgcatc ggatggctgc ggccggcgtc
    10381 ggtaagaata attttgcgcg cgaaagccac ctcgcgtcca cgcggattga gtgtgacttc
    10441 cccgcctgct gcgaccgccg caagttgcag accccagcag gagccgaaca tcggaattgc
    10501 gcgcgcgaag acttcacgca cgaagtcgat ctgccgcaat gacgcgacct cgcgctggta
    10561 gatgttcagg gacgatccgg taattgccac gccgtcatac gaatcgagcg gcgcgggcac
    10621 catagctccc tcgtccgccg gcgtgcagat atccaccttt gcatcctgcg cgatcgcgcg
    10681 cagcacgtcg gcataacttt ccgctggcgt ggcgcccgct gactcagcga tgcggcgacg
    10741 gcccgctgaa gcattgcctt cggcgaccag cagccgccgc atcgctcacc ctccaaggtc
    10801 gccgcgaatg gccgccagcg tcagttgctc aaactccgcc tcggtcaacc gccgcggatt
    10861 tgcagccgct gtcggatcct tcgcggcatc ggctgcaatc gccttgacgt cgacttccct
    10921 gacgccgagt tcggccaggg tatggggaac accgagtttc tttctcaaag cgagaatcca
    10981 gtccagtaca gcggaaaaat cccgacccgg caaatccagc gtgcgggcaa tgtgtggaat
    11041 cttgtcggcg atggccgttt tattgcagac cagcacatac ggaaaaatca cgccgttggt
    11101 caggccatga tgcgtgtcgt agcgcgctcc gacgggatgg gatacggagt gaatcgcacc
    11161 cagccctttt tggaacgcgg tcgcccccat cgaggcagcg gcgaacatgt aggcgcgcgc
    11221 ctccagatcg ccgccattgt caacggcgcg cggcagatag gtgtcgacga tcctcaatcc
    11281 ctccagcgcc acgccatcgg caagcggatg aaaggcgtcc acgcaatagg cctcgaagca
    11341 atgcgccagt gcgtccatgc cggtcgccat ggtcagaaac ggcggaaggc cggcggcaag
    11401 ctccggatct tcgatggcga ctttcggcat catcaacgga tgaaagatga tcttcttttc
    11461 gtgggtttcc tcacgcgtga tgacgccggc ccggccaacc tctgaacctg tcccagcggt
    11521 ggtcggcacc gcgatgatag gagcgatgcc ttttgaattg gcacgcgtcc accagtctcc
    11581 gacgtcttcg aaatcccaga ccggccgcga ctgcggcacc atgaacgcca cgcacttgcc
    11641 gacgtcgagc gccgaaccgc cgccgaccgc gatgacgccg tcatggcccc ccgcgcgaaa
    11701 ggcagcaatc cccgcgttaa ggtttgtctc cgttggattg ctcttcacgt ccgcgaaaat
    11761 gcgcacctca attcctgcat cgcagaggtg cttgacgaca ccggcgatca gcgccgtatt
    11821 ggcaagcccc ttgtcggtca ccagcaatgg cttggcgata tccacagcgc ggcaggtttc
    11881 gggcagttca gcgattcgtc cggcgcccgt gataacgcgg gtcggaaaat tccacgcgcc
    11941 agcgatgttg gtagccatgg catgctctca gtgttcgatg gggaggttga atgattcggc
    12001 gagagccgga ttcagatgat ttcgaagtaa cgcgccagct cccagtcggt gattgccctt
    12061 tggaattcac gctgctccca gcagcgggtc attgcgtaat gatcgacaaa gacgtcgccg
    12121 aacaggctgc gcgcggcgga agacgcggca agcctttccg ccgcctcaaa gagcgtgcga
    12181 ggcagcgacc gttcgggcgg atggctgatg tcataggcgt ttcccactat cggagaaccg
    12241 ggttcaatcc ggtgctggat tccccagagg cccgaaccga gagccgcggc cagcgccaga
    12301 taagggttga tatcggcggc agcgattcga tattcgacgc gctgagcgga aggcgcaccc
    12361 ggaatgactc ggagcgcaca ggtgcggttc tcgattcccc acgttgcgtc ggtcggcgcc
    12421 caaaaccccg ggatcaatcg cgaatagctg ttcacggtgg aggccaccat ggcgagaacc
    12481 tcgggcaaga gcgcctgctg cccgccgacg aaccagcgca tggtgtcgga catgttgccg
    12541 ggccgatctg ctgcaaaaaa ggccggcttc ccctccagat cacgcagcga tagatgcaga
    12601 tgtccggatt gtcccggata agagttcgac cacttcgcca tgaaggtgag cattttaccc
    12661 tggcgctcgg ccaaaacctt cgagaacgtc ttgaagatta ccgcgcggtc cgcggctgcc
    12721 atgacatcgg tgtacttgat cgccgcttcg aggactcccg gaccggtctc cgtgtgaaga
    12781 ccttcaagtg gcatgtccat gtcgtcgcag agcttcaaca gcccatgatg gaagtcagac
    12841 tccaccgatg acctcaacat cgaataaccg aaccagcctg gcgtcagatt tttcagactg
    12901 cgatagcctt tctcgcgcac gctgtgaggt gtctcgtcga agacaaaaaa ctcgaactcg
    12961 gccgccgccg aagctgtgaa accaagcttc gcggcgcgct cgaccacgcg gcccagcagg
    13021 ttgcgggggc aaagatcggc ggcagcaccc tcgaactcgc cgaggaaaaa cagcatatcg
    13081 ccttccgtcg gcacatcgcg acaggagttg gggatgacgc gcgccgtcgc atcgggaaac
    13141 gcggtatgcc agccggtgaa cctcgacttg tcgtaaagct gatcctgaga gtcccaaccg
    13201 aaaatcacgt cacaaaaatt gaatccggcc tcaagcgccg cgaagaactt gtcccgcgcg
    13261 atgtatttgc cgcggataac cccgtcgaga tcgaccacgc caagcttgac gtgggacagt
    13321 ccacgggcat ccactaacgc tcgtgcgtcc tcggcggttt tgacgtctct ggaagccatg
    13381 ttttcttcat atcttggtgg gtggttcaag acggtgcacc ggatcggcgg ccggacggca
    13441 cgaaaccgct cctgtgtcag gccggaaagt tctggtgcag cacctcgtcg caggcggtga
    13501 tgaacatgtc ggcgttgtcc ttcgtgaaac agagtggagg gcgtatcttt agcgtattgc
    13561 catatggacc ggccgcgcca agcaggatgc cgcgctggcg cagagtgttg atgagatggc
    13621 tcgcgatctc aggtgccggc gccttggtct cgcggtcatt cacaagttcg agcccgataa
    13681 agagacccgc accacgcacg tcgccgatct ggatgtggcg gttaccgatc tcacggagcc
    13741 catccattag gtagtcgcca actctgaagg cattttcggt gaggccctcg tcgcggatcg
    13801 tatcgaggac ggcaagaccc gcggcggcgg ccaccggatt gcctccgaag gtgttgaagt
    13861 actttgcttc cgccgtgaac cgatcgagca atgccgcgcg tgtcgcaatg ccccccatgg
    13921 ggaatccgtt acccatcggc ttgcccatgg tcacgatatc aggcacgacg tcgtgccgtg
    13981 cgaaacccca caacgaggcc ccggtccggc cgaacccggg ctgcacctcg tcggcgatga
    14041 agatgccgcc atgcgcccgc gcagcctcaa cggcggcggt gagaaaaccg ggcggatcgg
    14101 cgtagactcc gtcacttgag aagattgtgt cgacgagaag accggcaaag cgaatcccgt
    14161 tccgtttcag gtcggcgacc gccgtcgtca ccgacgcggc aaaacgctga ccgggatcgc
    14221 tgccggcgca gcggaacatc tccggcgccg gaatagcacg gacgtgtggc ggcagcgctt
    14281 gccctggaag gctcgacggt gagacacccg tcaccgcggc ggtgttaccg tgataagccg
    14341 tctcggtgac gataaagcct gtgccgccgg tggcagcttg cgcaattcgg agcgcaaggt
    14401 cgttggcctc gctcccggtg cacgtcagaa ccaggtggtc gattgcatcg gggaatgtcg
    14461 cgagcagtcg ttccgcgtac agatcgacaa cgtcgttcag gtagcgcgtg tgggtgttca
    14521 ggaggcctgc ctgtttgcaa atcgcctcaa cgacacgcgg gtgcgaatgc ccaaccgacg
    14581 ggacattgtt gtagaggtcg agatagcggc ggccgtccgc gtcatacatc cagacgcctt
    14641 cggcgcgctc catgcgcaac ggctcctgat agaacaatac ggacgctgaa ccgaaggaac
    14701 gcaaccgccg cgccacggct tcgcgtaagt cggtactcat ggctgacgcg ttcttttgat
    14761 cgaaagcgtt cagtgccaga atttcctttt cctgtggcat ctgcgccttc cccatcggcc
    14821 tcccgcaggc ccaccggaag gcctaacgtc gtcatatcga ccgcccacta ctttgcggca
    14881 atgtgggaat ttccacaaca tgaacacgcc ctgagcaacg acttgcagga gtgtcagccg
    14941 aatgctgatc gacaccaaac ccattgctga attggacgat ctttaacgca attctgcggg
    15001 caatgtgccc agcgcatgag cagagcgccg cataaaatat gagggaagtg ccacaaaatt
    15061 agtgtggaca tccaaatctc gctgcgaaag gattgcgcgc ggtggaatct ggctcggtcg
    15121 gcaccattcc gaggatgcgt caatgaatag cgagcggttc ccggtacccg acgctgcacc
    15181 cgcggagaag cggcttccga ggagacaaga aggcgaccgc ttctcgaagg ttggacgcca
    15241 ccaacacatc a
//
LOCUS       DASWGM010000366         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_720249,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000366 DASWGM010000000
VERSION     DASWGM010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720249"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VGA15_10685"
     CDS             complement(<1..423)
                     /locus_tag="VGA15_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX9588194.1"
                     /translation="MQRITCPERSDWRTTAEETGFDFHTIDGERYWDERAYYAFSLEE
                     IEHQIEAPSGEIDAMCLELVGNAVDDENYLRRLKIPEAFWPLIAASWHHDESSLYGRL
                     DLSYDGRGPAKLLEYNADTPTSIFEAAVFQWTWLEQAIE"
     gene            complement(944..1354)
                     /locus_tag="VGA15_10690"
     CDS             complement(944..1354)
                     /locus_tag="VGA15_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588195.1"
                     /translation="MAEKPNREFGRRKPVAIQTTVPVKRSNHVALLLMGTFAIGGGAY
                     ALMPGENCEQSRPGMAAPSGPQANAECPPRGSSSSGGHGSSGGSSRSSFFGGDTSSGH
                     SSTGTASDSGSGGVTRGGFGGFAHAFTSHFSGGG"
     gene            complement(1357..1761)
                     /locus_tag="VGA15_10695"
     CDS             complement(1357..1761)
                     /locus_tag="VGA15_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX9588196.1"
                     /translation="MILQSLAGLPAFLVYFCTALVAVVAYLFVYTRVTPHDEFQLIRD
                     NDPAAAIALGLSLLGFVLPLVSAIAHSANVLDCLIWSAIALVVQIAVYFIVKIPVPNL
                     SARIANGEVAAAIWLGLASLAAGALNAASMIY"
     gene            complement(1918..>2223)
                     /locus_tag="VGA15_10700"
     CDS             complement(1918..>2223)
                     /locus_tag="VGA15_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9588197.1"
                     /translation="VVNLDTKTVEVGGQRVHLTGKEYQMLELLSLRKGTTLTKEMFLN
                     HLYGGMDEPELKIIDVFICKLRKKLANASEGRNFIETVWGRGYVLREPHEVEERIPA"
BASE COUNT          451 a          671 c          694 g          407 t
ORIGIN      
        1 ctcgatcgcc tgctcgagcc aggtccactg aaacaccgcc gcctcgaaaa tcgaagtcgg
       61 tgtatccgcg ttatattcca gcagtttggc agggccgcgt ccgtcataac tgaggtcgag
      121 ccggccgtag agactgctct catcgtggtg ccagctcgcg gcgatcagtg gccagaatgc
      181 ttccggtatc ttcaatcggc gcaaataatt ttcatcgtcg acggcgttgc cgacgagttc
      241 gaggcacatg gcgtcgatct caccggatgg cgcctcgatc tggtgttcga tctcctccag
      301 cgagaacgca taataggcgc gctcgtccca atagcgctcg ccatcgatgg tgtggaaatc
      361 aaacccggtc tcttccgcgg tcgtgcgcca gtcgctgcgt tcggggcaag taatacgctg
      421 catgggtcga tgttagagca tgatccggaa aagtggaaac cggttttccc tccggacaaa
      481 agcgaagtgc gtgcccaaag atcaggccca aacagaaacc cgagttactc agcgcaaccg
      541 gatcaagcgc tagcctccgc catgcccatg cccgtgccca ccatggccgt gcccgtgccc
      601 gtggaaccga tgcaggccgc cgccaaaacc gccataggcg gggcaacttt caatgagcct
      661 tccatccggg ccgcgcgttc gatagggaac gcgtccgggt ccgcaatcta cccgcggcac
      721 aaaacccatc gatacggtgc cgacggccgc ggtacccatc atggtcagga tgacacggcg
      781 cgaacgtttc ataggatcac ctgctcttgc acatgcgaag agttttggga gagccaagcc
      841 ctgatattca agctctggca ttgaggcaga gttgcgattg acgggcaagc caaagcgaac
      901 cgtttcacag gatcgtgcgg gacgccgccg gtcggtctcg tcgtcagccg ccgccggaaa
      961 aatgcgacgt gaacgcatgc gcaaacccgc cgaatccgcc gcgcgtgacg ccgcctgatc
     1021 cggaatcgga cgcggtgcct gtcgaagaat ggccggacga ggtgtctccg ccgaagaaac
     1081 tgctgcgcga cgatccgccg gaagaaccgt gaccgccact ggatgaagat ccacggggtg
     1141 ggcattcggc gttggcttgc ggccccgacg gcgctgccat tcccggtcgg ctttgttcgc
     1201 agttctcgcc tggcatcagc gcgtaggcgc caccgccaat ggcaaaggtg cccatcagca
     1261 gcagcgccac atggttggag cgcttcaccg gtacggtggt ttggatcgca accggcttgc
     1321 gtctgccgaa ttccctgttt ggtttctcgg ccatgatcaa tagatcatcg aggcggcgtt
     1381 gagcgcgccg gcggcgagcg atgcgagccc gagccagatc gcggctgcga cttcgccgtt
     1441 agcgatccgc gccgacaggt tcggtaccgg tatcttgacg atgaaataga cggcgatctg
     1501 aacgacaagc gcgatcgcgc tccagatcag gcaatccagg acattggcgg agtgggcaat
     1561 ggcgctgacc agcggcagca cgaaaccgag caggctgagg ccgagcgcaa tcgcggccgc
     1621 cgggtcgttg tcgcggatca gctggaattc atcgtgcggc gtgacgcggg tatagacaaa
     1681 gaggtaggcg acgacggcga caagggcggt acagaagtag accaggaacg ccggtagtcc
     1741 cgccagtgat tgaaggatca tttggaagcg cccctaatcg tccgacatca cggtcgcacg
     1801 atccgtcggt cggtgcacgc gaacagccgg ttcaatcccc aaacaaaaac cccgccattt
     1861 gcggcggggt ccagttcggg aggagagagc ccgacgggct ctcgggtaga atagtgatca
     1921 ggcggggatc cgctcttcga cctcgtgcgg ctcgcgcagc acatagccgc ggccccagac
     1981 ggtctcgatg aagttgcggc cttcggaggc gttggcgagc ttcttgcgca gcttgcagat
     2041 gaagacgtcg atgatcttca attccggctc gtccatgccg ccatagagat ggttgaggaa
     2101 catttccttg gtcagcgtcg tgcctttgcg gagcgagagc agctccagca tctggtattc
     2161 cttgccggtc agatggacgc gctggccgcc gacctcgacc gtcttggtgt cgagattgac
     2221 cac
//
LOCUS       DASWGM010000367         5951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_162722,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000367 DASWGM010000000
VERSION     DASWGM010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162722"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VGA15_10705"
     CDS             <1..1436
                     /locus_tag="VGA15_10705"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX9588198.1"
                     /translation="TQQTSLAPLFANLSVAAGLDGLPAPVQQAVGQLLAQRTSLDQGL
                     TGNDIKQAFQNSGLFLEASLASGSLSPSSSSAPDLKAALIVLRQVLTTSLNGATATPS
                     TLATGSAPAPAAVVQQGATPAAAIVPQATTLPTPVVQGAMTPVAIALAQATLLSIQGS
                     MQPVIITVGLETPTMASPTLVPLLAPEISAFGASLQAAAPPVPQDVLGFGAANPVIST
                     ASTPADALRAAASAAALNLLQEAVQAGPLTAGNPSGLGFENSQSVSLLPALTGTRLLK
                     IDDAEFARSNVPPPPISGALPAAQPVMAAKLVSNSSSETAMHHLLADTDGAIARQTLL
                     QVASLPDRADTATARVDHAGPRWNFEIPFATPQGTAMAQFEISRDGGGGHETEAAKRV
                     WRARFSLDVEPAGPVHALVSLSGERTSVRIWAERPATTAQLRAGAAQLSQALSKAELQ
                     PGDIVIRDGTPPHSAPPRAGHFLDRAL"
     gene            1433..1699
                     /locus_tag="VGA15_10710"
     CDS             1433..1699
                     /locus_tag="VGA15_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX9588199.1"
                     /translation="MSVETKHQLAVALHYDKTGAPRVIAKGKGTIGAKIIEVAKAHDI
                     PIEENEVLAGALSNVEIGDEIPAELYKAVAEVLIFVLRLSGRIR"
     gene            complement(1953..2144)
                     /locus_tag="VGA15_10715"
     CDS             complement(1953..2144)
                     /locus_tag="VGA15_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588200.1"
                     /translation="MADIAIRFWDLMARGRDQKLFPHFGEAGTAVFAVEEVEYDRHDR
                     TPSFYHHHAIIFLGVQEVI"
     gene            complement(2427..2672)
                     /locus_tag="VGA15_10720"
     CDS             complement(2427..2672)
                     /locus_tag="VGA15_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HEX9588201.1"
                     /translation="MWAPSGSVLTAYDEEHLVTYLRLLDADVEGADWREVARVVLHLE
                     PEHESDRARGAFESHLSRAKWMTEHGYRLLLRGGASA"
     gene            complement(2672..2836)
                     /locus_tag="VGA15_10725"
     CDS             complement(2672..2836)
                     /locus_tag="VGA15_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588202.1"
                     /translation="MPAGRSVSAMLASYDGDIRAYLVATLGWLKYVVLEAPAAKMRSA
                     TWMSMKAFRL"
     gene            3011..4123
                     /locus_tag="VGA15_10730"
     CDS             3011..4123
                     /locus_tag="VGA15_10730"
                     /inference="COORDINATES: protein motif:HMM:NF022763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HEX9588203.1"
                     /translation="MRTPLIGALAATLVGCSCPLPPQASMESCTDANRFACFDRTAAS
                     WPVEPEQASFETDSETTEIESAIAAKTEKPSSAHARDRAHLVTKTAKPTMIAAKVEPP
                     SSRIPLPTRSLKTQPQPASNAAAYSDTTRANITDSHPTGGAVANSNIRTMQEQVAAAT
                     EVAERMTVGAVVPAPEPKANDKDRSSHSETVLRGDAEKTAPASPNEPPTGSAQNQPSD
                     TAWVVSETTSPVDYSPLITAVIQSMSSVKDAPNTLAVRCRGPRTELLLRTEGVWRASR
                     ASEVQVDYQINDQPFVRLQWTMSADGKTASYKDDAVELLRSLPEDARLKIGVSDRQGP
                     GQEATFQLTGLDSVRKKIGLACKWPPTADKMSSGKR"
     gene            complement(4446..5195)
                     /locus_tag="VGA15_10735"
     CDS             complement(4446..5195)
                     /locus_tag="VGA15_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588204.1"
                     /translation="MGLEIFTLRERPDLRPLIFASDLQSVWPEFMTHSAAAELYFSPS
                     MFDRYLDYAFAGVADGKVVARAFSVPFAFNTDGRAELPDGGWDQVIRWAHHDRMIGRA
                     PNALSALEISMLPEARGSGNSLALLGAMKACAKVKGFGEVFAPVRPNQKHLQPRTSMR
                     DYVNIVRSDGFPIDGWLRTHLRAGGRFVKIAPYSMTIVGRLADWSLWTGMPFDRSGEL
                     LVAGALSPVMVSLEQDYAVYVEPNVWVQHPV"
     gene            5450..5578
                     /locus_tag="VGA15_10740"
                     /pseudo
     CDS             5450..5578
                     /locus_tag="VGA15_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5686..>5951
                     /locus_tag="VGA15_10745"
     CDS             5686..>5951
                     /locus_tag="VGA15_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEX9588205.1"
                     /translation="MINRRHVLRLLAAASVAPRSALANVAPQRSEIREDLAKHFTDEG
                     TAGTFVGYKIDDYLIIASDKERSGEAMLPASTFKIPNSLIALETG"
BASE COUNT         1246 a         1851 c         1762 g         1092 t
ORIGIN      
        1 cgacccagca gaccagtctc gcgccactgt ttgccaatct aagcgttgcc gccgggctgg
       61 acggcctgcc ggcgccggtc cagcaggcgg tcgggcaact gctggcgcag cggaccagcc
      121 tcgatcaagg cctgaccggc aatgacatca agcaggcgtt tcagaattcg ggcctgtttc
      181 tggaagcctc attggcgtcc ggatcgcttt cgccgtcctc atcctccgcg ccggatctca
      241 aggcggcgct gatcgtgctg cgccaggtgt tgacgacgtc gctcaatggc gcgacggcga
      301 cgccaagcac gctggcgacg ggtagcgcac cggcaccggc ggctgtcgtg cagcagggcg
      361 cgacaccggc ggccgccatc gttccgcaag ccacgacgtt gccaacgccg gtggtgcagg
      421 gggcaatgac gccggtggca attgcgctgg cgcaggccac gctgttgtcc attcaaggct
      481 caatgcagcc cgtcatcatt accgttgggc tggaaacgcc gacgatggca tcgcccacgc
      541 ttgtgccgct gctcgcgcct gagatctcag ccttcggagc ttcgctccaa gccgcggcgc
      601 cgccggtccc gcaagatgtg ctcggcttcg gcgctgcgaa ccccgtcata tcgaccgcat
      661 cgacgccggc agatgccctg cgtgccgccg ccagcgccgc cgcgctcaat ttgctgcagg
      721 aagccgtgca ggccgggccg ctgacggcgg gaaatccgtc cgggcttggg ttcgagaaca
      781 gccagtcggt ttcgctgctg ccggcgctga ctggaactcg cctgttgaag atcgatgacg
      841 ccgaatttgc ccgcagcaac gtgccaccgc cgccgatcag cggcgcattg ccggcggcgc
      901 agccggtcat ggcagccaag ctggtatcga attcgtcgtc cgaaacggcc atgcatcatc
      961 tgctcgccga taccgacggc gcgatcgcgc ggcagacatt gttgcaggtg gcctcgttgc
     1021 cggatcgtgc cgataccgca actgcgcgcg tggaccatgc cgggccgcgc tggaatttcg
     1081 aaattccgtt tgcgacgccg cagggaaccg cgatggcgca gttcgagatt tcgcgcgatg
     1141 ggggcggcgg ccacgagacc gaggcggcca agcgggtttg gcgagcccga ttctcgctcg
     1201 acgtcgagcc cgccggtccg gtccatgctt tggtatcgct cagcggcgag cggacatcgg
     1261 tgcggatatg ggctgagcgg cctgcgacaa cagcgcaatt gcgcgcgggt gctgcacaac
     1321 tgagccaggc gctcagcaag gccgagcttc agcccggcga catcgtgata cgagacggca
     1381 cgccgccaca ctccgcgccg ccccgcgcgg ggcatttcct ggatcgcgcg ctatgagcgt
     1441 cgagaccaag catcagctcg cggtcgcgct gcattacgac aagaccggtg cgccacgggt
     1501 catcgccaag ggcaaaggga cgatcggtgc gaaaatcatc gaggtcgcca aggctcatga
     1561 catcccgatc gaagagaacg aggtgctggc cggcgcgctt tcgaacgtcg aaatcggcga
     1621 tgaaatcccg gccgagctgt acaaggcggt ggcggaagtg ttgatcttcg tgctgcggct
     1681 gtccgggcga attcgctagg acgtgaaccc ataaattccg agagttcggc ggacgtccgt
     1741 tttgttgagc attccggact caaatcggac gtcgcgcgag gtccgaaaag ccacaagcag
     1801 actcatgcgc cgcagcaatc cacagctcta ttcgatcgcc ttgtcccggc caactattcg
     1861 acgcgatcga ggactcgcgc gaccgggaca gcggaccatt tgccgtgccc gcgcgccgtt
     1921 ggaatgccta cctcgttgag ccgggctgtg atctagatca cctcttgcac cccgagaaaa
     1981 atgatagcgt ggtgatgata aaacgaaggg gttcgatcat gccgatcata ttcgacttcc
     2041 tctaccgcga atactgccgt gcccgcctcg ccgaaatgcg gaaacagctt ctggtctcgg
     2101 ccgcgagcca tgaggtccca gaagcgaatt gcgatgtcag ccatcctggc ggctccgctg
     2161 gtcgggatag cggttttggc gacgatagga cagccacaca gcacgaagct cgccaaattc
     2221 actgagccgg cgcggctaaa gacggctgag ggtggagtgg ctgacggcat aggaccggcc
     2281 gatgtccacc aaggaccacc ggcctcccgt cgcgcaatgg cttcttggcg ctggtggtga
     2341 gttagtttcg gattgcggcc caaataacca cccctttagc tttggcgcgc tcccggccct
     2401 cggtggtgcg acgtagcagg gtcgctttaa gcggacgcgc caccgcgcag caggagccgg
     2461 taaccgtgct ccgtcatcca tttggcgcgc gataaatggc tctcgaacgc cccccgggcg
     2521 cgatcagatt catgttccgg ctcaagatgc agcaccaccc gtgcgacttc acgccagtca
     2581 gcaccttcca catccgcatc gagcaggcgc agatacgtga cgagatgttc ttcgtcgtaa
     2641 gccgtcaaca cagaaccgga gggcgcccac atcagagacg aaatgccttc atactcatcc
     2701 acgtggcgga gcgcatcttc gccgcgggcg cctccagcac aacatattta agccagccca
     2761 gcgtcgccac gagataggcg cggatatcgc catcgtagct cgcaagcatc gcggagacgc
     2821 ttcgacccgc cggcaaagct actcacctac cgccgcgttg cgccgacatt aaccatgcaa
     2881 gcgcttctca cggcgaaggc cgaacgctta atgcgcgctt aacatctttg cacaaaagat
     2941 agacgaccag cgccaaacat tatgacgcag caagaattgc gagcggaact cagcctctaa
     3001 gggggattgc atgaggacgc ctctcatcgg cgcgctggct gcgaccttgg tcggctgcag
     3061 ctgcccttta ccgccgcaag cgagcatgga atcatgcacg gacgcgaacc ggttcgcctg
     3121 tttcgacagg acggctgcca gttggccggt tgaaccagag caggcgtcat tcgagaccga
     3181 ttccgagaca acagaaatcg agtccgccat cgcggcgaag accgaaaagc cgtcgtctgc
     3241 tcacgcccgc gatagggccc atctcgtcac gaaaacggcg aagcccacca tgatcgcagc
     3301 gaaagtcgag ccaccatcat ctcgcatccc actcccgact cgctcattga agacacaacc
     3361 gcagcccgca agtaacgccg ccgcctattc cgatacgacg cgagcaaaca tcacggattc
     3421 gcatcccaca ggcggagccg ttgcgaattc caatatcaga acgatgcagg agcaggtggc
     3481 agcagccacg gaggtcgcgg agcgaatgac ggtcggcgcc gtggtcccgg cgccggaacc
     3541 gaaagcgaat gataaggacc gttccagtca ctcggaaacc gtgctgcgcg gcgatgccga
     3601 aaagaccgca cctgcgtcac cgaatgaacc gcctacggga agcgctcaaa accagccgtc
     3661 ggatacagct tgggtcgtaa gtgaaacaac ctctcccgtg gattactccc ctctcatcac
     3721 ggcggtgatc cagtcaatgt ccagcgtgaa ggatgcacca aatactctgg ctgttcgctg
     3781 tcgtggacca cgcaccgaac tcctgcttcg cacggaggga gtgtggcgcg cgtcgcgcgc
     3841 cagcgaagtt caagtcgact atcagatcaa cgaccaaccc tttgttaggc tacagtggac
     3901 tatgtccgcg gatggaaaga ctgctagtta caaggacgat gcagttgagc ttcttcggtc
     3961 gctaccagaa gacgcacggc tgaaaatcgg cgtgtccgat aggcagggcc ccggccaaga
     4021 agcgacgttc caactcaccg gattggatag cgtccggaag aaaattggat tggcatgtaa
     4081 gtggccaccg acagcagaca aaatgtcatc ggggaaacga tgaaccacaa ctctgtacct
     4141 gttgtgatcg ggagtcgacg gggctgacgt gaaggacggg tccagcgcgc ctcgccgttg
     4201 gtgtacgcgg ccgatcgagc ccgctactgc gacattctcc aacaggtagg accgcaggga
     4261 gaatgatctc caacctcgca gatgaatcgc tgcccggcag cggccaaatg tgcgagttgg
     4321 gtcaatcgcg tcattcggac catgcgcgga ttatttccgg tctaccccga aagcagacaa
     4381 tccaatggcc gtcggcactt cgcaaaagtg ccaagcagac tcacaccatt tagcgggaat
     4441 ggttgtcata ccgggtgctg aacccaaaca ttcggctcga cataaactgc gtaatcttgt
     4501 tccagcgaaa ccatcaccgg cgagagcgca cccgccacca aaagttcgcc agatcgatcg
     4561 aacggcattc cggtccaaag agaccaatca gccaacctgc cgacgatcgt catagaatag
     4621 ggtgcgatct ttacaaacct tccgccagcg cgaaggtggg ttctcagcca accgtcaatc
     4681 gggaagccat cagaacgcac aatgttaaca tagtcgcgca tcgacgtgcg tggctgtaga
     4741 tgcttttggt tcggtcgcac cggcgcgaac acttctccaa atcctttgac tttcgcgcag
     4801 gctttcatag cacccaatag cgccaacgaa ttgccgctgc cccgggcttc gggtagcatg
     4861 ctgatttcta gcgcgctcag ggcattcggt gcgcgtccaa tcatcctatc atggtgagcc
     4921 cagcggataa cctgatccca gccgccatcg ggcagttcgg cgcggccgtc ggtattgaac
     4981 gcaaatggca cgctaaacgc tcgcgcgact accttgccat cggctacgcc agcgaaagca
     5041 tagtcgagat agcgatcaaa catcgatggc gaaaaataga gctctgctgc cgcactgtgt
     5101 gtcatgaact ccggccatac agactgtaaa tccgaagcaa aaatcagcgg ccgaagatcg
     5161 ggtcgctctc gtaatgtaaa gatttccaga cccatgatgc ctccctagcc tgtcacatga
     5221 tggcatctct gcggcccttc gcctaggtcg tgcggccagc gacaaatggc atctgctcaa
     5281 cgtgagcatc aattaagcgg atgtccgggt tgggggtcat tttggtcgat tttggcatgt
     5341 ccgcggcagg tccgcttaat gggtcatctc ggaagtgtcg gttgtccggt tttgctgttg
     5401 gttttgcttt tggacgtgat tcaagctcca aaacagggag cctcgaaaca tgcggaatga
     5461 actcagcgac catgagtggg atgttatcga cccgatgctt cccaacaagc cgcgtggcgt
     5521 tccccgggtt gacgaccggc gcgtcctgaa cggcatcttt tgggtgttgc gctccggcta
     5581 cgcggtaatg agcccgcgtc acagtctcat ttttccggtt gtcattgttt caccacgatg
     5641 tcatcagcat cgtcgccgcc gttccccgtc atatcggatg gtgccttgat caatcgtcgc
     5701 catgtcctca ggttacttgc cgccgcgagc gtcgcgcccc ggagcgcgct cgccaacgtc
     5761 gcgccgcagc gcagcgagat ccgcgaggat ctcgccaagc atttcaccga cgagggaacg
     5821 gctggcactt ttgtgggcta caagatcgac gactacctga tcatcgccag cgacaaggag
     5881 cgttccggag aggcgatgct gccggcctcg accttcaaga ttccgaattc gctgatcgcg
     5941 ctggagaccg g
//
LOCUS       DASWGM010000368         4860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_720389,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000368 DASWGM010000000
VERSION     DASWGM010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720389"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /locus_tag="VGA15_10750"
     CDS             complement(<1..2231)
                     /locus_tag="VGA15_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX9588206.1"
                     /translation="MTLSVRTTLLLSSSASLLLGLGGAAMSQTTAPAGGGSTALPKIE
                     VIEPRRAQPPRRPRTRVTTGQRRETPAAPPPPTEAQVVAGKNDKFDEVRQNIVAPIGA
                     TSYQINHQAIEALPQGSNASLDKVLLQFPGVTQDSSASGELHVRNEHANIQYRINGIM
                     LPDGVGAFGQILDTGIVGSLALLTGALPAQYGLRTAGVLDIQTKADAFNNSGSVSVYG
                     GSHGTITTSLEYGGTVGQTQYFMSGRYFGSNLGIENPTPANEAIHDRTSQEKAFLYLS
                     TVIDPTSRLTFMSGVSNGTYQIPNRPGQSPAFTAFGVTDFNSALLNEHQNEFNQFNVV
                     AYQKSGDGIDYQLSYFNRYSQLHFMPDPVGDLVFNGVASNVYRQSYVNGIQEDTAYRI
                     GYAHTLRFGFSVSAERSLVTNISTVLPLADPANPAAGTIDAPSTVFDSSSKTGWLLGT
                     YLQDEWKITNNLIMNAGLRFDQMYQYVDANQISPRVSLTWMPFDSTTFHAGYARNFTP
                     PPQVVAAPTNLALVQGTSQQPAVPLNDPVLPERSHVFDVGVIQKILPIPGLEVGIDGY
                     YKIARDLLDDGQFGAAYVLSGFNYDRANNLGLELKSSYTTGNFRIYGSLAWARQLGTN
                     VVSNQYLFDPERLAFIASHYIYTDHAQVLTASAGASYLWNGTRFSASMIYGSGLRSGF
                     ANTDHLPSYTQVNAGVSREFLFPGWTKPTTVRFDVVNLFDKIYAIRDGSGIGVFAPQF
                     GPRR"
     gene            complement(2390..2806)
                     /locus_tag="VGA15_10755"
     CDS             complement(2390..2806)
                     /locus_tag="VGA15_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEX9588207.1"
                     /translation="MKWFRSNIKHGSRLALFALAVQFALSFGHFHGIAVQAAPATQSG
                     PVLSDPLYANSLAAPDAVSEAGQPSPSDQDSDRQPADACAICAVMALANTLLFATPPL
                     LLLPQAVEFLYLTTDAEFVHLNSARVAFQPRAPPAS"
     gene            complement(2970..>4860)
                     /locus_tag="VGA15_10760"
     CDS             complement(2970..>4860)
                     /locus_tag="VGA15_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588208.1"
                     /translation="REDREPLSSVAAVVIDKSPSQNFGERTRETAQAQEALVDSLKKI
                     KGLEVRVVEAGQADGETDGTRLFGALSSALSDVPIDRVAGAFMITDGRVHDIPANATA
                     LGFQAPVHALITGRKDERDRRIAISAAPRFGIVGQTQTITYRLDDQGVSGDRAKVVVR
                     RDGEVINERTVQSGQTVNIEIDIKHAGPNIVEIEASPLDNELTLVNNRAVVAIDGVRD
                     KLRVLLVSGEPHSGERTWRNLLKSDASVDLVHFTILRPPEKQDGTPINELSLIAFPTR
                     ELFQQKINEFQLIIFDRYARQGVLPIAYFDNIARYVRAGGAVLVSAGPDYASTTSIWR
                     TPLDSVLPAEPVGVTEKPFYAHLSDAGKRHPVTRGLEGSASEPPRWSRFFRTVDTRNA
                     SSPPVMTGADGKPLLLLSRFGEGRVALLLSDHIWLWARGYEGGGPHLDLLRRMSHWLM
                     KQPDLDEEALRLQIQGKDLVVLRQTMADSVTPVTVTSPSGATRELTLSASEPGTWRST
                     IPASELGLWQATDGTLKALINVGPTNPKEFSEVTSTTEMLKPLAQATGGDTRRVVDGS
                     SIDLPRIVPVRASGVFRGDGWLGVKMRDASVVKGVGVLPLFAGLIGLLLLLGAFAATW
                     LREGR"
BASE COUNT          918 a         1435 c         1591 g          916 t
ORIGIN      
        1 cgccgtggcc cgaactgcgg cgcgaacacg ccgatgccgg atccgtcccg gatcgcgtaa
       61 atcttgtcga acaggttcac gacgtcgaag cgaaccgtgg tcggtttggt ccatcccggg
      121 aaaagaaatt cgcgagacac cccggcgttg acctgggtgt aggagggcag gtgatcggta
      181 ttcgcaaagc ccgagcgaag cccgctgccg tagatcatcg aggcgctgaa gcgcgtgccg
      241 ttccacagat aagaggcgcc cgctgacgcg gtcaggacct gcgcgtggtc ggtgtagatg
      301 tagtggctgg cgatgaaggc aagacgctcg gggtcgaaca ggtactggtt cgagacgaca
      361 ttggtgccga gctggcgcgc ccaggcgagg ctgccataga tacggaaatt gccggtggtg
      421 taagacgact tgagctcgag gcccagatta ttggcccggt catagttgaa gccggacaat
      481 acataggccg caccgaattg cccatcgtcg agcagatcgc gcgcgatctt gtagtagcca
      541 tcgatgccga cttcgagacc gggtatcggc aagattttct gaatcacgcc gacatcgaac
      601 acgtgggatc gctccggaag caccggatca ttcagcggga cggcgggctg ttgcgatgtg
      661 ccctgcacca gcgccagatt ggtcggggca gccaccacct gcggcggcgg cgtgaagttt
      721 cgcgcatagc cggcgtggaa cgtggtgcta tcgaacggca tccaggtcag gctgacccgc
      781 gggctgatct ggttggcatc gacgtattga tacatctggt cgaagcgcaa cccggcgttc
      841 atgatcaggt tattggtgat cttccactcg tcctgaagat aagtgccaag cagccagccg
      901 gtcttggagc tggaatcaaa cacggtcgat ggcgcatcga tcgtaccggc agcagggttc
      961 gccggatcgg cgagtggtaa tacggtggat atattggtga ccaatgagcg ctcagcgctc
     1021 acggaaaatc caaaccgaag ggtatgcgcg tagccgatac gataggctgt atcctcctga
     1081 atgccattga catagctctg gcggtagacg ttcgaagcca caccattaaa aaccagatca
     1141 ccgaccgggt caggcatgaa gtgcaactgg ctgtagcggt tgaaatagga aagctggtag
     1201 tcgataccgt cgcccgattt ctggtaggca accacgttga actggttgaa ttcgttctgg
     1261 tgttcgttga gcagcgcgga attgaagtcg gtcaccccaa aggccgtgaa ggccggcgac
     1321 tgtcccggcc tgttgggaat ttgataggtc ccattcgata cgccgctcat gaaggtcagg
     1381 cggctggtgg gatcgatcac tgtcgagagg tagagaaatg ctttttcctg cgaggtgcgg
     1441 tcgtggatcg cttcattggc cggcgttgga ttttctattc cgagattgct tccgaaatag
     1501 cgcccggaca tgaaatactg ggtctgtccg accgtgccgc catattcgag actcgtggtg
     1561 atcgtgccgt ggctgccgcc atagacactg acgctgccgc tgttgttgaa ggcgtcggcc
     1621 ttggtctgga tgtccagcac gcctgccgta cgcaagccat attgtgccgg cagcgcacct
     1681 gttagcaggg caaggctgcc gacgatgccg gtatcgagga tttgcccgaa ggcgccgaca
     1741 ccgtcgggca gcatgatgcc attgatgcga tattgaatat tggcgtgctc gttgcggaca
     1801 tgcaattcgc cgcttgcgga tgagtcctgc gttacaccgg ggaactgcag cagcaccttg
     1861 tccagcgacg cattgctgcc ctgcggcagc gcttcaatcg cctggtggtt gatctgatac
     1921 gaggtggcgc cgattggcgc gacgatattc tggcgaacct cgtcaaactt gtcattcttg
     1981 cctgcgacca cttgcgcctc ggtgggtggc ggtggtgcgg ccggcgtttc gcgacgttgc
     2041 cctgtggtca cgcgcgtccg gggacgacgc ggcggctgtg cacgccgcgg ctcgatgacc
     2101 tcgatctttg gaagcgcggt gctgccgccg cccgcgggcg ctgtggtctg cgacattgcg
     2161 gcgccgccca ggccaagcag cagcgacgct gaactggaga ggaggagagt ggttcttaca
     2221 gaaagcgtca ttattcccga tcctgataaa gcggctgcca gattcgtcgt cgtcaacgaa
     2281 ttcgaaaagc gcttgccgtc gatgtgcgac ggaccgatct cgatttaacc ctggggggat
     2341 cggccgcgca tgacgcggcg acccttcagg gatcacaaat caatcgatgt caggaggcgg
     2401 ggggagcgcg gggctgaaag gcgacgcgtg cggagttcag atggacgaac tcggcatcgg
     2461 tggtcaggta caggaattcg accgcctgcg gcagcagcaa cagcggcggc gtcgcaaaca
     2521 acagagtgtt ggcgagcgcc ataacggcgc aaatcgcgca agcgtcggcg ggttgccggt
     2581 cggaatcctg gtcggacggc gacggctgac cggcctcgct gaccgcatcg ggcgcggcaa
     2641 ggctattggc gtaaagaggg tcggatagca ccgggccgga ttgagtagcc ggcgcggcct
     2701 gcacggcgat cccatgaaaa tgcccgaacg acagcgcgaa ctgaacggcc agcgcaaaca
     2761 gcgccagacg cgagccgtgc ttgatgttcg accggaacca cttcatgcag cgatgcacct
     2821 tgcgtcggga ggccacagca tcccggccac cctgacgtta tattataaca tcaggaatat
     2881 cagcagggtg tcaaccgcgg tctcttcgca cgtgcgaaaa ttcgcaaggt ctgtgtgatt
     2941 cacgcaacgt caggcggcgc gagccaccat cagcgtccct cgcgcagcca ggtggccgcg
     3001 aacgcgccga gcagcagcag caagccgatc aggccggcga acaagggcag cacgccgacg
     3061 cctttgacga cactggcatc gcgcatcttc acgcccaacc agccgtcacc gcggaacacg
     3121 ccggacgcgc gcaccggcac gatgcgcgga aggtcgatgc tggagccatc gacgacgcgc
     3181 cgcgtgtcac cgccggtggc ctgcgccagg ggtttgagca tttcggtggt ggaggtgact
     3241 tccgaaaatt ccttcgggtt ggtcgggccg acattgatca gcgccttcag cgtgccgtcg
     3301 gttgcctgcc acaggccgag ctcgctcgcc ggtatggtcg agcgccatgt cccgggctcg
     3361 ctcgcgctca gcgtcagctc gcgcgtggcc cctgacggcg aagtcaccgt caccggcgtg
     3421 acgctgtccg ccatggtctg gcgcagcacc accaggtcct tgccctggat ctgcagacgc
     3481 aatgcttctt cgtcgagatc aggctgtttc atcagccaat gcgacattcg ccgcagcagg
     3541 tcgagatgcg gtccaccgcc ctcatagccg cgcgcccaca accagatgtg gtcggacagc
     3601 agcaaggcga cgcgtccctc gccaaatcgc gacagcagca atagcggctt gccgtcggcg
     3661 ccggtcatga ccggcggact agaggcgttg cgggtgtcga cggtgcgaaa gaaccggctc
     3721 cagcgcggcg gttcgctcgc cgaaccttcc aggccgcgcg tcaccgggtg gcgtttgccg
     3781 gcgtcgctca gatgcgcgta gaacggtttt tcggtgacgc caactggctc ggccggcaga
     3841 accgaatcca gcggcgtgcg ccagatgctg gtggtcgagg cgtagtcggg acccgccgac
     3901 accagcaccg cgccgccggc gcgcacatag cgggcgatgt tgtcgaaata ggcgatcggc
     3961 agcacgcctt ggcgcgcata gcgatcgaag atgatcaact ggaattcatt gatcttctgc
     4021 tgaaacagtt cgcgggtcgg aaacgcgatc agggacaatt cgttgatcgg cgtcccgtcc
     4081 tgcttttcgg gcgggcgcag aatcgtgaaa tgcacgagat cgacgctggc gtcggacttc
     4141 agcaggttgc gccaggtgcg ctctccggaa tgcggctcgc ccgaaaccag cagcacccgc
     4201 aatttgtcgc gcaccccgtc gatcgcaacc accgcgcgat tattgaccag cgttagttca
     4261 ttgtcgagcg gagaggcctc gatttcgacg atgttcggtc cggcgtgctt gatgtcgatc
     4321 tcgatgttga cggtctgccc gctctgcacg gtgcgttcgt tgatcacctc gccgtcgcgg
     4381 cgcaccacga ctttggcgcg gtcgccgctg acgccctggt cgtcgagccg gtaggtgatg
     4441 gtctgggtct gtccgacaat gccgaaacga ggtgcggcgg aaatcgcgat gcgccggtcg
     4501 cgttcatcct tgcgtccggt gatcagggca tgcaccgggg cctgaaatcc gagcgccgtc
     4561 gcgttggcag gaatgtcatg cacccgcccg tcggtgatca tgaaagcacc ggcgacgcga
     4621 tcgatcggga cgtcggacag tgccgacgac agtgcgccga ataatctggt gccatcggtt
     4681 tcgccgtcgg cctggccggc ttcgacgacg cgcacctcca gccccttgat ctttttgagg
     4741 ctgtcgacca gcgcctcctg cgcttgcgcg gtttcgcggg tgcgctcgcc gaaattctgg
     4801 ctggggctct tgtcgatcac gaccgcggcc accgatgaca acggctcgcg gtcctcgcgg
//
LOCUS       DASWGM010000369         6860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1410400,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000369 DASWGM010000000
VERSION     DASWGM010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410400"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VGA15_10765"
     CDS             complement(<1..512)
                     /locus_tag="VGA15_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588209.1"
                     /translation="MSQVKRDMERIEGLHFQAIEVALQAKALKECSFPGHSDWVLDQG
                     DDEANRLAYAIGTNRWENGEIDGEHNLRVRIRNTILELEPGLDAGRADPGAETPVRPM
                     AEPSSPTSTLPPPSVVLTTRLSDGCSAVSNASVAVLACLTKPNPRAGHFGAKIAQAAA
                     GRRANNTASR"
     gene            complement(986..1294)
                     /locus_tag="VGA15_10770"
     CDS             complement(986..1294)
                     /locus_tag="VGA15_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588210.1"
                     /translation="MRLLLGAVAAVGSLALAIAAFAQSTPTPATAVPVQSNPSPAEPV
                     PLGKRFACQTVSQGLKGQEQRDQVQLCVAQAHLDCLKQAIDQKIVGPQRKDFVKSCVQ
                     "
     gene            1727..2050
                     /locus_tag="VGA15_10775"
     CDS             1727..2050
                     /locus_tag="VGA15_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9588211.1"
                     /translation="MTESIQIQLKKGALELCVLALLARRESYAYEIASTLSAGVDMGE
                     GTIYPLMRRMQNDGLVDTRLAESNSGPPRKYYRLTSSGRAAFVAQKREWRAFTDAVNQ
                     LLGET"
     gene            2050..2622
                     /locus_tag="VGA15_10780"
     CDS             2050..2622
                     /locus_tag="VGA15_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEX9588212.1"
                     /translation="MNRVAFLRILRDGLAGLPAHEIDDILADYAAYFDEAHASGRSEE
                     EVATALGDPRRLARELRAETGLRRWENHRSLGNSATALLALGGLAAVDILLLLPLLFA
                     VMLTVLVVGLVIFVLGIVGIGLLLSLLKIGQFASIAAMVLRALAGIGLITGSVGCGAL
                     LLLALNGAVKMLGNYARLHYRLLKPEQHAA"
     gene            2652..3419
                     /locus_tag="VGA15_10785"
     CDS             2652..3419
                     /locus_tag="VGA15_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEX9588213.1"
                     /translation="MNKRLAWIAMVSFCISAACGLLAVLTATAWVGGDDRSWLTTIRI
                     CVTMPWTKTASSYRLTDRNDGVRASEPRVVDLEWRGGDAVKVDIPARVHYQPGEKAQA
                     SVGGDAELVSHVRMRDGRLEWDTVVNCFSADDLVVRITGPAVTAWTLNGSGKLDLSDI
                     KQDALRITMRGSGTVTASGEARQASLDVAGSGRADLGRLVTQQASARIRGGAEIDLAP
                     REDADISIAGSAAVRLHGAVTRMQSHISGSGQIKQVP"
     gene            3591..4604
                     /locus_tag="VGA15_10790"
     CDS             3591..4604
                     /locus_tag="VGA15_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9588214.1"
                     /translation="MNPNKSSSPNVSAAERRRTKATLAGLFGLLLMVAMIPVGVIAFD
                     APTSPPAMVSMARSLDRINVGDVPAPRQFRARDGASLQYYVYPAAPDRVAVLVHGSAG
                     PGTSMHVLAEALRAAGLTAYVLDIRGHGGSGRRGDIDYIGQIDDDLSDFVAQLGPAGS
                     GETRTLVGFSAGAGFSIRFAGGPYGLLFDRYVFLAPILPGAPTWRPNAGGWTNIALPR
                     IVTIAWLDRLGIHWFDGFDVISYAVSRDPSRDTTASYSYRLSVNFGGRGYETYLKNIR
                     RPAAVLVGDADEQVVADQFAPLLQRLGVNIPVTIVPDMKHADMIAAPAALPAVVRAIS
                     AQE"
     gene            complement(4760..5644)
                     /locus_tag="VGA15_10795"
     CDS             complement(4760..5644)
                     /locus_tag="VGA15_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588215.1"
                     /translation="MAFFKRELSPVERFERALREKQAARQKLADRLGLAETALGEQRD
                     EAERLAVAGAANAKLARAEARMRTVEDRAKTLRAALVEFDEQVVATERALADAKAQRD
                     RDRAADEIEALAAAIEQAAPGFGAGAAALVEAVTKSTASVREATMFSTSVDEMRREVL
                     SAADLICWELRSTAVRTRAGNANIALLAPPEPKQLPAPEIERQLIYTLSPLRWREGSE
                     VRRVPAFALVGLPKMLLPVALRHQHVDYLNARRVQTLIHVHGNGEFNSDPHPDDPQLV
                     DLDALAAEENESAQADVA"
     gene            complement(5814..6185)
                     /locus_tag="VGA15_10800"
     CDS             complement(5814..6185)
                     /locus_tag="VGA15_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX9588216.1"
                     /translation="MSLQKRWRSLFVLGAISVLIGGGAHAQNLDQGKPAARLFADGCA
                     TCHHSARGLAKGRFRLTLYLFLQQHYASNSSSAWALTSYLESVDSPQRGRSRAAAAKP
                     SPPATGTSRFSLRPPASVPGH"
     gene            complement(6542..6769)
                     /locus_tag="VGA15_10805"
     CDS             complement(6542..6769)
                     /locus_tag="VGA15_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588217.1"
                     /translation="MILKYPKLRLSDSVVLGQFIRKPIAHEELAAQITKAPGIDGRTP
                     TGDENEVVGNVDALEVKAQYEAMLLKRGKKN"
BASE COUNT         1284 a         2113 c         2070 g         1393 t
ORIGIN      
        1 tgccggcttg ctgtgttatt tgctctacgc cctgctgcgg cctgagcgat tttagcgcca
       61 aaatggcctg cccttggatt tggtttcgtt aggcatgcca acacggccac gcttgcgttg
      121 ctcacggcgc tgcagccatc cgataatctc gtcgtcaaca caacggatgg tggaggcaag
      181 gtgcttgtcg gcgaacttgg ctccgccatc ggcctgactg gcgtttcagc cccaggatca
      241 gcgcggccag catcgaggcc gggctcaagc tctaagattg tgtttcggat tctaactcgg
      301 aggttgtgct cgccatcaat ttcgccgttc tcccagcggt ttgttccgat cgcataggcg
      361 agcctattgg cctcatcatc gccctggtct aacacccaat cagaatgacc gggaaaactg
      421 cattctttca aggcttttgc ctgcagagct acctcgatgg cttgaaaatg gaggccttca
      481 atgcgctcca tgtctctttt gacctgactc atgcgcaact cccacatcga acaggcccgt
      541 gaactaagat gacggggcgg ctttttaatg gcgagcacaa cctcagagtt aggagccgag
      601 aaataatctt agagcttgag cccggcctcg atgctggccg cgctgatcct ggggccgaaa
      661 cgccagtcag gccgatggcc tgaaaccttc cattggcgtg attatggaac atttctgtcg
      721 gcgcgatttt tcttgttcaa tttggaatcc cgacacaaaa gagaagcatc cgcgttgctt
      781 gcccgcgtcg acgaggtagt tgaatagatc ggctaattga tgcgctgcat gagtctcttg
      841 tgggtcaaac gcgtcatctt taccgcgcgc cggtcacttc cgatctacgc cgacgagcag
      901 acattctcag tgtcggtcgg ccggcccaag cgtgactgag aagcacccca aaccactagt
      961 cgtctttggc catgcccaat agggcttatt gcacgcaact cttcacaaaa tccttccgct
     1021 gtggacctac gatcttctga tcaatcgcct gttttaggca gtcgagatga gcctgtgcga
     1081 cgcagagctg cacctgatct cgctgctcct gtcctttcaa tccttgcgaa acggtttgac
     1141 aggcaaatcg ttttccaagg ggcacgggct cagcaggtga aggattggat tgcaccggaa
     1201 cagcggtcgc aggcgtcggc gtggattgag caaatgcggc tatcgccaag gcgagcgagc
     1261 ctaccgcagc gacggcgcca agcagaagtc tcatgagcgg cctcccttga taccttaaac
     1321 ttaatgttcc tctctgaacg tgctgtgaac gggctctgaa accaaattca aaggcggtcg
     1381 tgcgacgctt cgcgaatggc tttactagac acgtttgtgc aatcggggaa aaccgatcgt
     1441 ccgatcggac cggccgtgaa catctcaatg aagcactgat gcaaagcccg cgatttgccg
     1501 cttccgggat tgtgccgagt acattcattg tcgtggcttc gatcggcggc gctgctagcc
     1561 ccgtttcgat tctatcgaaa cggaaaaggc ctggtccggc gaaaccattg tatttggcat
     1621 gacggcaatt gccccgtgag gtagcgatat tgcattgaca ggtaccttgc attagtatgt
     1681 accttgctat atgtcttcga ttgaagccca ctgccgcacg ggtccgatga ccgagtcgat
     1741 ccagatccag ctcaagaaag gcgcgctcga attgtgcgtg ctcgcgttgc tggctcgtcg
     1801 ggaaagctac gcctatgaga tcgccagcac gctttctgcc ggcgtcgaca tgggggaggg
     1861 gacgatctac ccgctgatgc ggcggatgca gaatgacggg ctggtcgata cccgcttagc
     1921 cgaatcgaac agcggcccgc cgcgaaaata ctatcggctc acctcaagcg gccgcgcggc
     1981 tttcgtggcg cagaagcgcg agtggcgggc gttcacggat gccgtcaacc agttgctcgg
     2041 agaaacctga tgaaccgcgt cgccttcttg cgcatcctcc gcgatgggct cgccgggctc
     2101 ccggcccacg agatcgacga catcctcgcc gactacgctg cctatttcga cgaagcccat
     2161 gcgtccgggc gaagcgagga ggaggtggcc acagcgcttg gcgatcccag gcgactggcg
     2221 cgggaattgc gcgccgaaac cgggctgcgc cgctgggaaa atcaccgcag cctcggcaat
     2281 tccgcgactg cattgctggc gcttggcggg ttggcggcgg tcgacattct gttgctgctg
     2341 ccgctgctgt tcgccgtgat gctgaccgtg ctcgtcgttg gcctcgtgat cttcgtcctc
     2401 gggatcgtcg gcatcggatt gttgctgagc ctgctcaaga tcggacagtt cgcctcgatc
     2461 gccgcgatgg tgctgcgcgc cttggcgggc atcgggctga tcaccggcag cgtgggctgc
     2521 ggcgcgctgc tgctgctcgc gctcaacggt gcggtgaaga tgcttgggaa ctacgcgcgg
     2581 ctgcattacc gcctgctcaa accggaacaa cacgccgcct gaggccaacc ggtattcgca
     2641 agaggtgaca catgaacaaa cgtcttgcct ggatcgcgat ggtgtcgttt tgcatttcgg
     2701 cggcatgcgg gttgctggcc gtcttgactg caacggcctg ggtcggcggc gacgatcggt
     2761 cctggctaac cacaatacga atctgcgtta cgatgccgtg gacgaaaaca gccagctcct
     2821 atcgcttgac cgaccgcaat gacggcgtgc gtgcgagcga gccgcgcgtc gtcgatctgg
     2881 aatggcgggg tggcgacgcc gtcaaagtcg atattccggc gcgggtccac taccagcccg
     2941 gagagaaggc ccaggctagc gtcggtggcg atgcggaact cgtcagtcac gtgcgaatgc
     3001 gcgacggcag gctcgaatgg gacaccgtcg tcaactgctt ttccgccgac gatctcgtcg
     3061 tccggattac cggccccgcg gttaccgcat ggacgctgaa cggctccggc aaactcgacc
     3121 tctccgacat caagcaggat gcgttgcgca tcacgatgcg tggcagcggc accgtcaccg
     3181 caagcggcga ggcccgtcag gcgtcgctcg atgtcgccgg ctcaggccgc gccgacctcg
     3241 gcaggctcgt cactcaacag gcgagcgcac ggatccgggg cggcgcggaa atcgatctcg
     3301 cgccgcgcga ggatgccgat atctccatcg ccggcagcgc ggccgtgagg ctgcatggcg
     3361 ccgtaacgcg gatgcaatcg cacatatcgg gctcgggaca aatcaaacag gtgccataga
     3421 gcgttttcaa gcgaaagctt gccccggact tgatccgggg tggagaccgg ttcgcatcaa
     3481 gaaaacgcgt caaatcaaga atccagggcc ccgtttcgat tctatcgaaa cggaaaaagc
     3541 tccaggcccg tcgcaatcaa gcggtcgtgc gcaacacgga ggtgacatcg atgaatccga
     3601 acaaatcgtc ttccccgaac gtgagtgccg cggaacggcg caggaccaaa gcaacgctgg
     3661 ccggtctgtt tggcctgctg ctcatggtcg cgatgatccc cgttggcgtc attgcgttcg
     3721 acgcaccgac atccccgccg gccatggtct cgatggctag atcgctcgat cggatcaacg
     3781 tcggcgacgt tccggcacct cggcaattca gggcgcgcga cggcgccagc ctgcaatatt
     3841 acgtctatcc cgcagcgccg gacagagtcg cggttttggt ccacggctcg gccggtcccg
     3901 gcacaagtat gcatgtcctc gcagaggcgt tgcgcgccgc cggcttgacg gcctatgtgc
     3961 tggatattcg cggtcacggc ggctcgggcc ggcgcggcga catcgattac atcgggcaga
     4021 tcgatgacga tctttcggac tttgttgccc aacttgggcc cgccgggagc ggcgagaccc
     4081 gcacgctggt cgggttctcc gccggggccg ggttcagcat ccgcttcgcc ggcggaccct
     4141 atggcctgct gtttgaccgc tatgtgtttc tcgctccaat cctcccgggc gcgccgacct
     4201 ggcgaccgaa cgccggcggc tggaccaaca ttgcgctgcc gcgcatcgtg acgatcgcct
     4261 ggcttgatcg tctgggcatt cactggttcg acggatttga cgtcatcagc tacgcggtat
     4321 cccgcgatcc gtcgcgagac acgaccgcca gctattccta tcgactgtcg gtgaatttcg
     4381 gcggacgcgg gtatgagacc tatttgaaaa acattcgccg gccggctgcc gtgctcgttg
     4441 gagacgccga cgagcaggtg gtcgcggacc aattcgcacc cctgttgcag cgccttggcg
     4501 tgaatatccc ggtcacgatt gtgccggaca tgaagcacgc cgacatgatc gcagcgcccg
     4561 cagcgttgcc ggcggtggtc cgcgcgatct ccgcgcagga gtagcggcgc ggtggtcgca
     4621 ggaggcagag gcctcattcg gcgatgtcgg ttttgctcga aagtgcggac caccatcagc
     4681 gcgatcgggc caaagtcggc agcctagccc caaaactctg cagcagcggg cgatcgcctg
     4741 tcgttcatga tcagctcgct cacgccacgt cggcttgggc gctctcgttc tcttccgcgg
     4801 cgagtgcatc gagatcaacg agttgcgggt cgtccggatg gggatcgcta ttgaactccc
     4861 cgttgccatg aacgtgtatc agggtctgca cacgtcgggc attcagatag tccacgtgct
     4921 gatgccgcag cgcgaccggc agcaacattt ttggcagccc gaccagcgcg aacgccggaa
     4981 ccctgcgcac ctcgctgccc tcccgccaac gcagcggact gagcgtgtaa atcaattgcc
     5041 gctcgatctc cggagctggc agttgctttg gctcgggtgg cgcgagcagc gcaatattcg
     5101 cattgcccgc gcgtgtgcgc accgcggtgg atcgcaactc ccaacagatc aggtccgcag
     5161 ccgagagaac ctcgcgccgc atctcatcca cgctggtcga gaacatggtc gcttcccgca
     5221 ccgacgccgt gctttttgtg acagcctcca cgagtgccgc ggccccggcg ccaaatcccg
     5281 gggcggcctg ctcgatcgct gcagccagcg cctcaatctc gtcggccgcc ctgtcgcggt
     5341 cgcgctgcgc tttggcgtcg gccaatgcgc gctcggttgc gacgacctgc tcgtcgaatt
     5401 cgaccaatgc ggcgcgcagt gtcttggcgc ggtcctcgac ggtgcgcatc ctggcctctg
     5461 cgcgagcaag cttggcgttg gcggcgccgg ccaccgccaa gcgttcggct tcgtcgcgtt
     5521 gttctccaag tgccgtttcg gcgaggccca accggtcggc cagcttctgc cgcgccgcct
     5581 gcttctccct cagcgcacgc tcgaagcgct cgaccgggct gagctcacgc ttaaaaaacg
     5641 ccatgacaat atcctcgcga atcgagacca tccgccgaat cagattaaat gcgttcaaaa
     5701 cgtagtcgga aatgtggata acattgcggc tttagtcggc ggatagtctc gaccgaccct
     5761 caattcaatc ctccgagcaa agaggcggtg cttcggtcac gcgcctctcc gacctaatgc
     5821 cccggcaccg acgcgggcgg acggagtgag aaccgtgatg tgccggtcgc agggggcgac
     5881 ggcttcgccg cggcggctcg cgatcggccg cgctgtggac tatcgacgga ctccagataa
     5941 gaggtgagcg cccaggccga gctcgaattg ctcgcgtagt gctgttgcag gaacaggtat
     6001 agcgtaagcc tgaagcggcc cttggcaaga ccgcgtgcgc tgtgatgaca tgtcgcgcaa
     6061 ccgtccgcaa acagcctggc ggccggtttg ccctgatcta gattctgagc atgtgcccca
     6121 ccgccgatca gcacggaaat tgcgccgagg acgaatagac tgcgccaccg tttctgcaac
     6181 gacataagct ccgacgctac ttgcatgccc ccatcgccct tcgccgcaac aaaaccgccc
     6241 tcgtcatgtc aaacaaacgc cggcgttccc aacctgtacc ccaaagcagg gttatggtcg
     6301 aacaacgtca aaaaggcggc gacgcgctcg ggtcgcctcg ttggccagcg cgagagatcg
     6361 cggacgatga gcagcgcggt cgggcaagat gggccgctat cccccgtagc tctgcaccaa
     6421 actgcccgcc accagcgaca tttcctccgc cggttcgatg tgcttgggcg atatcgagat
     6481 ttgcggtgac aatctgtgcg ttggaagttg ccatcggcta ttggcgaggc ccctgattta
     6541 cctaattttt cttcccgcgc ttcagcaaca tcgcctcgta ctgagccttc acctcaagcg
     6601 catcgacgtt tccgacgact tcgttttcgt cgcctgtggg cgtacgccca tcgatgccgg
     6661 gcgcctttgt gatttgtgca gcgagttcct catgggcgat cggcttacga atgaattgtc
     6721 ctagtactac tgagtcagaa agccgcagtt tcgggtattt gagaatcatt tatggaggcg
     6781 aatcccactg aaacaagggg gctgggatgg gtctccacgg agcgaaagag attgaattgc
     6841 gttttacccg ctatgtcgag
//
LOCUS       DASWGM010000370         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_40599,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000370 DASWGM010000000
VERSION     DASWGM010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40599"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VGA15_10810"
     CDS             complement(<1..453)
                     /locus_tag="VGA15_10810"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9588218.1"
                     /translation="MTPKSHQPLTREQMAWRAAQDLPEGAYVNLGIGIPTLAASFVPP
                     GREVIFHSENGILGMGPKPEPGKEDLYMVDAGKNLTTLVPGGCFVHHADAFLMIRGGH
                     LDVSLLGAFEVSEKGDLANWTTEDPAFPPGIGGAMDLAVGAKEIRIIME"
     gene            complement(450..1118)
                     /locus_tag="VGA15_10815"
     CDS             complement(450..1118)
                     /locus_tag="VGA15_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEX9588219.1"
                     /translation="MDKQVKTTADAVAGVRDGSTVLVAGFGAVGVADNLLDALHEQGA
                     RELTIVANNSGNGDYGLARLINSGRVRKVICSYPRSGDYSAFLKAYRAKSLELELVPQ
                     GTISERMRCHAAGLGGFFSPVSAGTKLAEGKETREIDGRLHVFEKPLKGDIALLKANK
                     ADRWGNLTYIKSARNFNPVMAMAADLTVVEVDRIVELGEMDPEAVVTPSIFVDRIVVV
                     GAQA"
     gene            1398..>1876
                     /locus_tag="VGA15_10820"
     CDS             1398..>1876
                     /locus_tag="VGA15_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9588220.1"
                     /translation="MSTIKLTVNGKAVSAEVEDRTLLVQLLRENLNLTGTHVGCDTSQ
                     CGACVVHIDGRAVKSCTVLVGQANGANVTTIEGISKGDELHPMQAAFRDNHGLQCGYC
                     TPGMIMSAIDIVHRHSGGLDEDTVRHELEGNICRCTGYHNIVKSVLDAAGRMKVSQAA
                     "
BASE COUNT          367 a          583 c          569 g          357 t
ORIGIN      
        1 ctccatgatg atgcggattt ccttggcgcc gaccgcgaga tccatggcgc cgccgatgcc
       61 gggcgggaat gccggatcct cggtggtcca gttcgcaaga tcgccctttt cggagacctc
      121 gaaggcgccg agcaggctga catcgagatg gccgccgcgg atcatcaaaa acgcatcggc
      181 gtgatggacg aagcagccgc cggggaccag cgtcgtcagg ttcttgccgg cgtcgaccat
      241 atagagatct tccttgccgg gctccggctt tggacccatg ccgaggatgc cgttctcgct
      301 gtggaagatc acctcgcgcc ccggcggcac aaagctcgcc gccaaggtcg gaatgccgat
      361 gccgagattg acataggcgc cttccggcaa atcctgcgcg gcgcgccatg ccatttgttc
      421 acgtgtcaga ggctgatggg atttcggggt catgcctgtg ctccgacaac gacgatgcga
      481 tcgacaaaaa tgctcggggt gaccaccgct tcgggatcca tctcgccaag ctccacgatt
      541 ctgtcgactt ccacgacggt caaatcggcg gccatcgcca tcacagggtt gaaattccgc
      601 gccgacttta tgtaagtgag attgccccag cggtcggcct tgttggcctt gagcagcgca
      661 atatcgccct tcagcggctt ctcgaacacg tgcagccggc catcgatctc ccgggtttcc
      721 ttgccctcgg cgagcttggt ccccgccgaa accggcgaga aaaacccgcc aaggccggca
      781 gcatggcagc gcatgcgttc gctgatggtg ccttgcggga ccaattccag ctcaaggctc
      841 ttggcccgat aggctttcag aaaggcgctg tagtcgcccg agcgcggata ggagcagatc
      901 accttgcgca cgcgcccgga attgatcagc cgggccaggc cgtaatcgcc gttgccggag
      961 ttgttggcga cgatggtcag ctcgcgggcc ccttgttcgt gaagtgcatc gagcagattg
     1021 tctgcgaccc cgacggcgcc aaatcctgcc accaataccg tcgatccgtc gcgaaccccg
     1081 gctacggcgt cggcggtggt cttgacctgc ttgtcgatca cgtcaaaggg ctcctttggg
     1141 cggcattgtc gtttggtgtc cgtggcttat gcagggatca atcggccggg aatagatcat
     1201 caactgcggc gccgagaaag ccacaaattt gcagccgtga cccgcatcga gcccctgtgg
     1261 gtcgtccggg cgaatgccgg gattacagat gtaacgcaaa tggcagcacc gtgaccttcg
     1321 cgacgaaaat cctagttgca aagtccctcc cccgtgcctc taattggaat aattaaaagc
     1381 atcgggaggt atcaagcgtg tctaccatca aattgacggt gaacggtaaa gcggtctcgg
     1441 ccgaggtcga ggaccggacc ctccttgtcc agctcctgcg cgagaatttg aatctgaccg
     1501 gcacccatgt cggctgcgac accagccaat gcggcgcctg tgtcgtgcat atcgatggcc
     1561 gggcggtcaa atcatgcacc gtgctggtcg gacaggccaa cggcgccaac gtcaccacca
     1621 tcgagggcat ctcgaagggg gacgaactgc acccgatgca ggccgcgttc cgcgataatc
     1681 acgggctgca gtgcggttac tgcacgccgg gcatgatcat gtcggcgatc gacatcgtcc
     1741 accgccacag cggcggtctc gacgaggaca ccgtccggca cgaacttgag ggcaatatct
     1801 gccgctgcac tggctaccac aatatcgtga aatcggtgct cgacgccgcc ggccgcatga
     1861 aggtttcgca ggcggc
//
LOCUS       DASWGM010000371         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2115289,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000371 DASWGM010000000
VERSION     DASWGM010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115289"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGA15_10825"
     CDS             complement(<1..365)
                     /locus_tag="VGA15_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX9588221.1"
                     /translation="MTTSEVRRKPFYTSLSFQVLVGIAIAIVLGYLNPAKAIAMKPLG
                     DGFIRLITMIITLVIFCTVVTGIAGMEDMKKVGRVGGKALLYFEAVSTLALLIGLVVG
                     NVVRPGSGFNINPATLDAKA"
     gene            complement(433..>1600)
                     /locus_tag="VGA15_10830"
     CDS             complement(433..>1600)
                     /locus_tag="VGA15_10830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9588222.1"
                     /translation="ALEMAQENGKLLAWRKKEGERVQKGEPLLEIETDKAVVEVEAPG
                     DGLLAGVTAQVGAVIPVGETIAWLVAPGEKPPAKIASVVPAARASSSGARAVAAAPLV
                     AEHPATSSPQISPKARRLAKELGVDIGRVRGTGPGGVITTEDVQAFAASKQTVAPAAP
                     AAGETLSQVARLMAERTAQGWTSVPHFFLVRDVDAGALIDAQKKLDAEADKTHGGRAT
                     ITDLLIALLARTLAKHPRMNSSWTGEGIRPNPDINISVAIAVKDGVVGAVVHNANTLK
                     VSEISTRRLDLSERARAGRLRPADISGGTFTLSNLGMYKVDAFSAIITPPQAAVLAIG
                     AVADRVVATNGQPSVRPIMTMTLSSDHRVVDGARAAAFLGGLADAISQPEKWLN"
BASE COUNT          286 a          516 c          479 g          319 t
ORIGIN      
        1 gctttggcgt cgagcgtcgc cggattgatg ttgaagccgc tgcccggccg caccacattt
       61 ccgacgacca ggccgatcaa cagcgccagg gtcgagacag cctcgaaata cagcagtgct
      121 tttccgccca cgcggccgac ctttttcatg tcctccatgc cggcgatgcc ggtcaccacg
      181 gtgcagaaaa tcaccagcgt gatgatcatg gtgatcagcc ggatgaaacc gtcgccgagc
      241 ggcttcatgg caatggcctt ggcgggattc aaatatccca ggacaattgc gatggcgatg
      301 ccgaccaata cttgaaagga caggctggtg tagaagggct tgcggcgaac ctcggaagtg
      361 gtcataaaga cggctgaacc ctccgcgtcg cgagggtttc ggcgtgctgc ctcagaattt
      421 ttgccaagag tttcaattca gccatttctc cggctggctg atcgcatctg ccagccctcc
      481 gagaaaagct gctgcgcgag caccgtccac cacgcgatga tcgctcgaca gcgtcatggt
      541 catgattgga cgcacactcg gctggccatt tgtcgcgaca acgcgatcgg ccacagctcc
      601 aatggccaga acggcagcct gcggcggcgt gatgatggcg ctgaaggcgt ccactttgta
      661 catgcccaag ttgctgagcg tgaatgtccc gccagaaata tccgcagggc gcagacgccc
      721 ggcgcgcgcg cgttcgctta gatccagccg ccgcgtcgaa atttcggaaa ccttcagggt
      781 gttggcgttg tgaacgacgg cgccgaccac gccatctttc acggcgatgg ccacgctgat
      841 gttgatatcg ggatttgggc gaattccttc gccggtccaa ctcgaattca ttcgcggatg
      901 cttcgccaac gttcgtgcga gcagagcaat cagcaagtcc gtaatggtgg cgcgcccgcc
      961 gtgtgtttta tcggcttcgg cgtcgagctt tttctgtgca tcgatcagcg cgccggcatc
     1021 cacatctcgt accaggaaaa aatgcggcac cgaagtccag ccttgcgcgg tccgctcggc
     1081 catcaagcga gcgacctggc tcagcgtttc gccggcggca ggagccgcgg gcgccactgt
     1141 ttgtttggag gcagcgaacg cctgcacgtc ttccgtggtg atgacaccac cggggccggt
     1201 cccgcgtacg cggccaatgt ccacgccgag ttccttggca aggcggcgcg ctttcggcga
     1261 aatttgcggc gacgatgtag ctgggtgctc ggccacgagc ggggcagccg cgaccgctcg
     1321 tgccccgctg ctggaagcgc gtgccgcggg gaccacggat gcgattttcg ccggtggttt
     1381 ctctccgggc gcgaccaacc acgcgatcgt ttcgccaacc ggtatcacgg cgccaacctg
     1441 cgccgtcacg ccggcaagca gcccatcgcc gggcgcctca acttccacaa ccgccttgtc
     1501 cgtctcgatt tccagcagcg gctcgccctt ctggacgcgc tcgccttctt tcttccgcca
     1561 cgccagtagt ttcccgtttt cctgcgccat ttcgagcgcc
//
LOCUS       DASWGM010000372         8146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1977147,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000372 DASWGM010000000
VERSION     DASWGM010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977147"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1697)
                     /locus_tag="VGA15_10835"
     CDS             complement(297..1697)
                     /locus_tag="VGA15_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX9588223.1"
                     /translation="MSTSDLNESPSVPTRRRKRQNAALLVLAAGIFLFAAAAGGLYYT
                     LRPVTLRIAVGPAGSDDQKLVQALAQTFSRDASSVRLSPITTEGATESIALLAASKAD
                     LAVARGDLDLPANAESVAILRKNVVVLWAPSGLPPKGSKKTPAPKIKGIDDLAGHHVG
                     VIGRTQANVTLLRVILTESGVNAEKVAITQFGTNQIAEMARDPTIDAFMAVGPLDSKI
                     TTDAIAATAAAKGEPKFLPIDVSEALAQRHRLYESEEIPGSTFSSSPARPEDKVETVS
                     VNHLIIAPKTLSDTTVGTFVRQLFAVRQTLAREVPSAAKIEKPDTDKDAALPAHQGAA
                     AYIDGTERTFLERYSDYFWAAILVLSGLGSAGAWLRQYSKRDEKEQSTLHRDKLLAMI
                     SKVRQVESPEELTAMQCEVDGVLREALDSYDDGAIEEGDLSAIGLVLEQFHHAVSDRR
                     MAMGAAAPDLPRRRAL"
     gene            1834..2889
                     /locus_tag="VGA15_10840"
     CDS             1834..2889
                     /locus_tag="VGA15_10840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9588224.1"
                     /translation="MRILIATDAWHPQVNGVVRTLTSLARSASAFGAEINFLTPDGFA
                     SLGVPTYPGLRVALPNRREIARRIEAASPDAIHIATEGPIGWAVRAYCRRRKLAFTTS
                     YTTRFPEYIAARWIIPAGLSYAVLRHFHAAAAMTMVATASLRQELSARGFAKLGTWTR
                     GVDTGLFNPDEPAELDLPRPVFMTVGRVAVEKNLEAFLSLSLAGSKVVIGDGPQRALL
                     ERRYPKVRFLGEKTGKDLTAHLAAADVFVFPSRTDTFGVVQLEALACGTPVAAFPVTG
                     PLDVIADHAVGALDTDLRRACIRALGMSRETCRNFALERSWENSARQFIGNLSALQPS
                     RSLQPVRRVAASGALRS"
     gene            2942..3631
                     /locus_tag="VGA15_10845"
     CDS             2942..3631
                     /locus_tag="VGA15_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9588225.1"
                     /translation="MAEIIKLDGTTQLDFDREMVEQAYDRWAPIYDLVFGGVFSKGRK
                     AAIQTTNRIGGRVLEVGVGTGISLPQYAPHLRIFGTDISEAMLQKAKKRVAELRLKNV
                     EGLAVMDAERLEFPDNSFDVVMAQYVVTAVPNPEVALDEFARVLRPGGELIILTRVSA
                     DAGVRRFIEQRLQPVVRPLGFRTAEFAWSRYARWLAGARGMELVERRLMPPLGHFSLI
                     RFRKTGVAAAA"
     gene            3758..4387
                     /locus_tag="VGA15_10850"
     CDS             3758..4387
                     /locus_tag="VGA15_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588226.1"
                     /translation="MIKNFREQLRTQRWDDHRYYHHSRINQSLHFVSAFSFLVGYALL
                     FVDPVAAALVGWLVSMTTRQAGHFFFEPKGYDHINQATHDHKEEIKVGYNLNRKIVLM
                     TIWALSPLVLFFDPTLFGVFTPWSSPADFMRQVAKVWLAIGIGGLLFRTVHLFFIRDV
                     ETGLVWMTKILTDPFSDLRLYYKAPLFLLRGELIDPGLDLIDQGIEKHA"
     gene            complement(4415..6079)
                     /locus_tag="VGA15_10855"
     CDS             complement(4415..6079)
                     /locus_tag="VGA15_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9588227.1"
                     /translation="MDSPLPILSLAAAATAVAAAGFPKFQARLALSRAKHRSLGGHSK
                     MSRMVARLIPFYEFDINDFFCSDGAPAAVATQRQDGFFRLAHLYQDKFARGRQMTAQA
                     SEKISDLQFTEAYRVPFQYSRLVREHLGTGAFVQSAAGVTVTDLDGNVFYDLTGSYGV
                     NIFGNDFYKECIARAEGRAHALGPVLGPYHPVIVDNVNRLCAISGLDEVSFHMSGTEA
                     VMQAVRLARYHTRRSHLVRFAGAYHGWWGDVQPGVGNPISPHETYTLADMSERTLRVL
                     RTRRDIACVLVNPLQGLHPNGNAPADSALVDSSRTGHFDRAAYTAWLQSLRQVCTERN
                     IVLIFDEVFVGFRLAAGGAQEYFGVRADMVTYGKSLAGGLPVGAVCGRKDLMRRFRED
                     RPVDVCFARGTFNSHPYVMTAMDEFLNRLDDPLFRSIYDGLDDTWNERARRLNDMLTE
                     RDLPVRVCNLSSIWMVHYTEPSRYNWMFQYYLRLEQLALSWVGTGRLIFSLNYTDADF
                     AEVAKRFVSAAEKMKQDGWWWRDTSLTNKSIRRQILKEMLTRRRQR"
     gene            6114..6992
                     /gene="asd"
                     /locus_tag="VGA15_10860"
     CDS             6114..6992
                     /gene="asd"
                     /locus_tag="VGA15_10860"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046080.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEX9588228.1"
                     /translation="MTIRGLISALTQQEDLNFLLTNRIPRAALTRFMGWLSKIEQPLV
                     RDLSIACWRLFSDLDLSEARKTDFKSLHDCFTRELKPGLRPPDPDPSIVVSPCDAIIG
                     AFGAIADSELFQIKGAPYSLLDLLGDPALVEAHRHGRFITLRLTSSMYHRFHAACDCR
                     IEQVTFIHGDTWNVNPIALKRIEKLFCKNERAVIRTRLESGDALTLVPVAAILVASIR
                     LHFLDIVLNAESRGPTVFPCDVSVRKGDELGWFEHGSTIIVLAPDQFEFCDNVVDSAR
                     ILAGQPLMRRSLARPV"
     gene            7086..7865
                     /locus_tag="VGA15_10865"
     CDS             7086..7865
                     /locus_tag="VGA15_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9588229.1"
                     /translation="MGHQMARVPVLAAGGIVLRREKIPLIAVVRLRKRDEWVLPRGKL
                     DDGETPRDAAEREVLEETGHDVSVHEFLGTLVYESGGRSKVVHYWRMETNGGQAYELM
                     DDVRAVDWLPLDAAVERLSRTYERAFLANVGPLALEAAMLARVPRRPKAKPRVPAKPR
                     DRRAVAPRPLTIPISAPPQPDLAPSATLQADRQSVASDVVETKTPVGPTQSGTAPQIV
                     EADTAMPALEPEPSNDDTLEAAQGRRRNLVQKMRDWLRRAA"
BASE COUNT         1523 a         2478 c         2536 g         1609 t
ORIGIN      
        1 tctggacgag tgcaggtcac cggcgctgat gcgattgtcg ccattctcgc cgattctgag
       61 agccaagccg cggcgctcat gcaggaacag ggcatgacgc gctagaggtg acgcgctaca
      121 tcagctatgg catcgccgga ggggatccgg ttttgcgtcg ccgcgagggc ggtgacgatc
      181 gtaagtcgcc gatgcccggg ttgggtcatt agctccgttt ttatggtcag ccgatggatt
      241 ccggtctacc ccgatgaacg gacatgtccc gctggcgagg gtagcgagat ccccgcctaa
      301 agcgcgcgcc ggcgcggcag atcgggcgcg gcggcaccca tcgccatgcg tcggtcggag
      361 acggcgtggt gaaactgttc cagcacgaga ccgatggccg acaggtcgcc ttcctcgatc
      421 gcgccatcat cgtagctatc gagcgcttcc cgcaagacgc cgtccacctc gcattgcatc
      481 gctgtcaatt cttccggtga ttccacctgg cgcactttcg aaatcatggc gagcagcttg
      541 tcgcgatgca gcgtgctttg ttctttttca tcccgcttcg aatattgccg caaccaggcg
      601 ccggcggacc cgagccccga gagaaccagg atggcggccc agaagtaatc gctgtacctc
      661 tcgaggaacg tgcgctcggt gccgtcgatg taggccgccg cgccctgatg cgcgggcaat
      721 gccgcgtcct tgtcggtgtc cggtttttca atctttgcgg cactgggcac ttccctggcg
      781 agcgtttgac gaaccgcaaa cagttgccga acgaacgtac cgaccgtcgt gtcggacaaa
      841 gtctttggcg cgataatcag gtggttcacg ctgaccgtct cgactttgtc ttccggtctg
      901 gcgggcgagg agctgaaggt gctgcctgga atttcctccg attcgtagag ccggtgtctc
      961 tgggcgagcg cctccgacac atcgatcggg agaaatttgg gctcaccctt ggcagccgcg
     1021 gtcgcggcaa tggcgtcggt ggtgatcttg ctgtccagcg gacccacggc catgaacgca
     1081 tcgatggtcg gatcgcgtgc catctcggcg atctgattgg tgccgaactg ggtgatcgcc
     1141 accttttcgg cattgacgcc cgattcagtc aggatcacgc gcagcaatgt gacattcgcc
     1201 tgggttcgac cgatgacgcc gacgtgatgt cccgccagat cgtcgatacc cttgattttc
     1261 ggtgctggcg tttttttgga gcctttgggc ggcaggccgg aaggtgccca cagcacgacg
     1321 acgttcttgc gcaggatggc gacggattcg gcgttggccg gcaggtccag gtcgccgcgc
     1381 gcaaccgcaa ggtcggcttt cgaggccgcg agcagcgcaa tactttcagt ggctccctcc
     1441 gtcgtgatcg gcgacagccg aaccgaactc gcgtcgcggg aaaaggtctg cgccagcgcc
     1501 tgaacgagct tctggtcgtc gcttcccgcc gggccgacgg caatccgcaa ggtgacgggc
     1561 cgtaaagtat aatacaatcc gcccgcggcc gcggcgaaaa ggaatatgcc ggcggccaac
     1621 accagcagcg cggcattctg ccgtttgcgg cgcctggtag gcacgcttgg cgattcgttg
     1681 aggtctgatg tcgacattat ccagccattc tacacgaaat agttctaggc gacccccaaa
     1741 aaaggatgac tacacatcga tgacgtcaca cgcttgaaat atccttagcg gaacgtcggg
     1801 acttgagaat cagctagctc tcaggttccc atgatgcgca tcttaattgc gaccgacgcg
     1861 tggcatccgc aggtcaacgg cgtggttcgc acattaacct cgttggcccg cagcgcttcg
     1921 gcgtttggcg ccgaaatcaa ctttctgacc cccgacgggt ttgcctcgct gggtgtcccg
     1981 acctatccgg ggctgcgggt ggccttgccg aaccggcgcg agattgcccg cagaatagag
     2041 gcggcctcgc cggatgccat tcatatcgcg accgaggggc cgatcgggtg ggcggtgcgg
     2101 gcctattgcc gccgccgcaa gctcgctttc accacttcct atacgacccg cttcccggaa
     2161 tacattgcgg cacgctggat cattccggct ggcctgagct atgccgtgct gcgacacttt
     2221 catgccgctg ccgccatgac gatggtcgcg accgcctcgc tccggcagga actcagtgca
     2281 agaggttttg caaagctcgg cacctggacc cgaggcgtcg acaccggcct ctttaacccc
     2341 gatgagccgg ccgagctcga tttgcccaga cctgttttca tgactgtcgg ccgtgtcgcg
     2401 gttgaaaaaa acctcgaggc gtttctctcc ttgagcctgg ctggatccaa ggttgtgatc
     2461 ggcgatgggc cgcagcgggc gctgttggag cgtcgctacc cgaaggtgag atttttgggc
     2521 gaaaagaccg gtaaggatct gaccgcccat ctggccgcgg cggacgtctt tgtcttcccg
     2581 agccgcaccg acaccttcgg cgtcgttcaa ctcgaagcgc tggcctgcgg cacgccggtc
     2641 gccgcatttc ccgtaacggg tccgctcgat gtgattgccg atcacgctgt tggcgccctg
     2701 gatacggact tgcgacgcgc atgcatccgc gcactcggca tgtcacgcga aacctgccgg
     2761 aattttgccc ttgagcgttc atgggaaaac agcgccaggc agttcatcgg caatttaagc
     2821 gcattgcagc cgagccgctc gctgcagccc gtccgccgcg tggcggcgag cggcgcgttg
     2881 cgaagttgag ctaaacactg agtctgattt cagactctgc aacaaagggt tggagttccc
     2941 catggcagaa atcatcaaac ttgacggtac gacgcagctg gacttcgacc gcgagatggt
     3001 cgagcaagcc tacgatcgct gggctccgat ctacgacctc gtgttcggcg gagtgttcag
     3061 caaaggccgc aaggccgcta ttcaaaccac caacaggatc ggcggccgcg tcctggaggt
     3121 cggcgtcggt accggcatct cgttgccgca atatgcgccg caccttcgca tcttcggcac
     3181 cgatatttcg gaggcgatgc ttcaaaaggc caagaagcgc gttgccgagc ttcggctgaa
     3241 gaatgtcgag ggcctcgcgg tgatggacgc cgagaggctc gaatttcccg acaattcgtt
     3301 cgacgtcgtg atggcgcaat atgtcgtgac cgcggtgccg aacccggagg tcgcgctgga
     3361 tgagtttgcc cgcgtgctgc gcccgggcgg tgaactgatc atcctcaccc gggtcagcgc
     3421 cgatgccggc gtgcgccgtt tcatcgagca gcggctgcag ccggtcgtgc gtccgctcgg
     3481 cttccggacc gccgaattcg cctggtcgcg ttatgccaga tggctggcgg gtgcgcgcgg
     3541 aatggaattg gtcgagcgcc gcctgatgcc gccgctcggc cacttttcgc tgatccgttt
     3601 ccgcaagacc ggggtcgcag ccgccgccta gctcgcgcga gcaggcgttg gctgaccatg
     3661 acgttgtgaa aacgccatat gtcattatga tgttgtcaca cagcgtacat cgaatctcag
     3721 taaacgaatc ccgaacacag ttttggggga gcaggtgatg attaagaact ttcgggaaca
     3781 attgcgaacg cagcggtggg atgaccatcg ctactatcat cacagccgta tcaatcagag
     3841 tctgcatttc gtcagcgcct tcagctttct ggttggctat gcgctgttgt ttgtcgatcc
     3901 ggtggccgca gccctggtgg gctggctggt ctcgatgacc acccggcagg cgggtcattt
     3961 cttcttcgag cccaaaggct acgatcacat caaccaggcg acccacgatc acaaagaaga
     4021 gatcaaggtc ggctataatc tgaaccgcaa gatcgtcctg atgacgatct gggcgctgtc
     4081 gccgctggtg ctgttcttcg atccgaccct gttcggcgtg tttacgccct ggagttcacc
     4141 ggcggatttc atgcgccagg ttgccaaggt ctggctggcg atcgggattg gcggcctgct
     4201 gtttcggacc gtgcatctgt tcttcatccg cgatgtcgag accggcctcg tctggatgac
     4261 gaagatcctg accgacccgt tcagcgatct caggctttac tacaaggccc cgctgttcct
     4321 gctgcgcggc gaactgatcg atcccggtct cgatctgatc gatcaaggta tcgagaaaca
     4381 cgcctgaaga agcacgaacg aggacggcgg cgttttagcg ctgccgtctt cgcgtcaaca
     4441 tctccttcag gatttgccgg cggatgctct tgttggtgag cgaggtgtcg cgccaccacc
     4501 agccgtcctg cttcatcttc tcggccgccg agacaaagcg tttcgccact tccgcaaaat
     4561 ccgcgtcggt gtagttcagg ctgaaaatca ggcggccggt tccgacccag ctcagtgcga
     4621 gctgctcgag gcgcagataa tactgaaaca tccaattgta gcgggacggc tcggtgtaat
     4681 ggaccatcca gatcgacgac aggttgcaga cccgcaccgg caggtcgcgc tcggtcagca
     4741 tgtcgttcaa ccgccgcgcc cgctcgttcc aggtgtcatc gagcccgtca tagatcgaac
     4801 gaaacaaggg atcgtcgagc cggttcagga attcatccat cgcggtcatg acatagggat
     4861 gcgaattgaa ggtgccgcgc gcgaagcaga catcgacggg acgatcctcg cgaaaacgcc
     4921 gcatcagatc cttgcgtccg cacaccgcgc cgacgggaag tccgcccgcg agacttttgc
     4981 cataggtcac catgtcggcg cgcacgccaa aatactcctg ggcgccgccg gcggcgagcc
     5041 ggaagccgac gaagacttcg tcgaagatca ggacgatgtt gcgctcggtg cagacttgcc
     5101 ggagcgactg cagccacgcg gtgtaggccg cgcgatcgaa atggccggtc cgcgagctgt
     5161 cgaccagcgc ggaatcggcc ggcgcattgc cgttggggtg aagcccctgc agcggattga
     5221 ccagcacgca ggcgatgtcg cgccgcgttc tcagcacccg gagcgtccgt tccgacatat
     5281 cggccagcgt ataggtttcg tgcggcgaaa tcgggttgcc gacgccgggc tgcacgtcgc
     5341 cccaccagcc gtgataggcc ccggcgaaac gcaccagatg cgagcggcgg gtgtgatagc
     5401 gggcgagcct caccgcctgc atcaccgctt cggtgccgga catgtggaac gagacctcgt
     5461 cgagccccga aatcgcgcac aacctgttga cattgtcgac aatgacgggg tgatacggcc
     5521 cgagcaccgg gccgagcgca tgggcgcgcc cctcggccct ggcgatgcat tccttgtaga
     5581 aatcgttgcc gaaaatattg acgccatagg agccggtcag atcgtagaac acgttgccgt
     5641 cgagatcggt caccgtgacc cccgccgccg actgcacgaa ggcgccggtg cccagatgct
     5701 cccggaccag ccggctgtac tggaacggca cccggtaggc ctcggtgaat tgcaggtcgg
     5761 agattttttc cgaggcctgc gccgtcatct gccggccgcg ggcgaacttg tcctgataga
     5821 ggtgcgcgag ccggaaaaaa ccatcctgac gctgggtggc gaccgcggcc ggcgccccgt
     5881 cggaacagaa aaaatcgttg atgtcgaatt catagaacgg gatcagccgc gcgaccatgc
     5941 gcgacatctt cgagtgcccg ccgagcgagc ggtgcttggc tcgcgacagc gccagtcgag
     6001 cctgaaattt gggaaaacct gccgcggcga cggcggtcgc ggcggcggcg agagagagaa
     6061 tcggaagcgg agaatccatg ccaccagcgc taatcaatcc cgctgacaga ttcatgacaa
     6121 ttcgaggcct gatctccgct ctgacccaac aggaagacct gaactttctg ctgaccaatc
     6181 gtatcccgcg ggcggccctg acccggttca tgggctggtt gagcaagatc gagcaacccc
     6241 tggtccggga tctctcgatc gcctgctggc ggctgttttc cgatctcgat ctttccgagg
     6301 cgcgcaagac cgacttcaag agcctgcatg attgttttac gcgcgagttg aagcccggct
     6361 tgcgtccgcc cgatcccgat ccgtcgatcg tggtcagtcc gtgcgatgcc atcatcggcg
     6421 cgttcggcgc catcgccgac agcgaactgt ttcagatcaa gggcgcgccg tattcgctgc
     6481 tcgatctgct cggcgatccc gcgctggtgg aagcgcatcg acatgggcgg ttcatcacgc
     6541 tgcggctgac gtccagcatg tatcaccgct ttcatgcagc ctgcgattgc cggatcgagc
     6601 aggtcacgtt catccatggc gatacctgga acgtcaatcc gatcgcgctg aaacgaatcg
     6661 aaaagctgtt ttgcaagaac gaacgggcgg ttatccgcac ccggctggaa agcggcgacg
     6721 cgctgaccct ggtgccggtc gcggccatcc tggttgccag cattcggctg cattttctgg
     6781 atatcgtgct caatgcggaa agccggggcc cgacagtctt tccctgcgat gtcagcgtcc
     6841 gcaagggcga cgaactcggc tggttcgagc acggatcgac gattatcgtg ctggcgcccg
     6901 accaattcga gttttgtgat aacgtggtgg attccgcgcg cattttggcc ggtcagcccc
     6961 tgatgcgaag atcgctggcg cggccggtct agccgcttcg gcacgcgggc ctaaccaact
     7021 cgattttgcg accgcgcgac attatatgga tatggtgacg gccgaaacga ttcgaaatcg
     7081 cagacatggg acatcagatg gcgcgggtgc cggtattggc ggcgggaggc atcgtgctgc
     7141 ggcgggagaa aattccgctg atcgcggtgg tgcgcttgcg caagcgcgac gaatgggtgc
     7201 tgcccagggg caagctcgac gacggcgaga cgccacgcga cgccgccgaa cgcgaggtgc
     7261 tggaggaaac cgggcacgac gtttcggtgc acgagtttct tggaacgctg gtctatgagt
     7321 ccggcggacg gtccaaagtc gtacactact ggcgaatgga aaccaatggc gggcaggctt
     7381 acgagctgat ggacgatgtc agggctgtcg actggctgcc gctcgacgcc gccgtcgagc
     7441 ggctttctcg cacctatgag cgtgcgtttc tggccaatgt cggtccgctc gcgcttgagg
     7501 ctgcgatgct cgcccgggtc ccgcggcggc ccaaggcaaa gccgcgcgtt cccgcaaagc
     7561 ctcgcgaccg gcgggctgtc gccccccgac cgttgacgat accaatatcg gccccgccgc
     7621 agcctgattt ggcgccctcg gcgaccctgc aggctgaccg tcaatccgtc gcgagcgatg
     7681 tagtggaaac caaaacaccg gtcggtccaa cgcagtccgg aacggcgccg cagattgtgg
     7741 aagcagacac cgcgatgccg gcactggagc ccgaaccgag taacgacgat acgttagaag
     7801 cggctcaagg ccggcgcaga aacctggttc agaagatgcg ggactggctg cgtcgcgcag
     7861 cctgatccgg tcgtgcaagg cttgctgctc tgaaaaggct tgctgctccg aaaaggcttg
     7921 ctgctctgaa aagactttct tctctgaaaa aaggctttct actctgacgc gctccgcggg
     7981 gcgggccttc caatgacgcc gcttcccgcg agtcgacaaa actaacttcc gtgtcagaaa
     8041 ctgtcacact tgtcatttga aatcattgga tcctttcggt ttgctggtgg tcttcttgcg
     8101 acggcgtccg tcgaaggaat tcaaccacgg aggattggga tgaaca
//
LOCUS       DASWGM010000373         9328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1142767,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000373 DASWGM010000000
VERSION     DASWGM010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142767"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VGA15_10870"
     CDS             complement(<1..722)
                     /locus_tag="VGA15_10870"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEX9588230.1"
                     /translation="MLAKYSIRTKITAVVAFLLVAMSLMGALNIRQMYAINSSTIDIT
                     TSWLPSVRVLGELRAATITYRAIVRSHLLATEEATRQVQEDLLAKWINNTDKARKAYE
                     PLITSAEERVLYDEFSTSWAEYLAGVKEVLALSRKNEDKEARLLHAKASLAGVHADET
                     LQKDVLLNNKGADAAGSQAEQSSDEAIKMVLASLLLAIILGIGAAILLVRDVSTGIRS
                     IVAPMQALGSGDLTAQVPHQGET"
     gene            complement(898..2175)
                     /gene="otnK"
                     /locus_tag="VGA15_10875"
     CDS             complement(898..2175)
                     /gene="otnK"
                     /locus_tag="VGA15_10875"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX9588231.1"
                     /translation="MTLKLGCIADDYTGASDLANTLTRCGLRTVQTIGVPLSDLALPE
                     VDAVVVSLKSRSIDAGLAVTASRAAEKWLRGRGAPHVLFKICSTFDSTDAGNIGPVMD
                     ALRADCGETIVLVTPAFPETGRTVYQGNLFVGSVPLNESPLKDHPLNPMHDSNLLRVL
                     ARQSKTKVGLVDLAVIARGPDAVRAHLADLSTRGFGAAIADAVFARDLETIGVVALDH
                     RLSVGASGIGLGLARALVASSRVKSNAPDAMADAAVGGPAACLAGSCSQATLQQIANA
                     ERVMPVFHLDSEQAVAGKAEVQRALGWANEHLGKGPVLIASSATPDPVAALQARHGRE
                     AAGHAIEQAMADIAQGLVQSGVRRLVVAGGETSGAVVDRLTIPGFLVGAEIAAGVPVL
                     RAVGAKEGAMLLALKSGNFGGPEFFSDALKLMP"
     gene            complement(2172..2954)
                     /gene="otnI"
                     /locus_tag="VGA15_10880"
     CDS             complement(2172..2954)
                     /gene="otnI"
                     /locus_tag="VGA15_10880"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEX9588232.1"
                     /translation="MPRFAANLSMMFTEVPFLDRFEAAARAGFTAVEFLFPYDHPAEA
                     VGERLHRNGLTQALFNLPPGNWEAGEKGFAALPGRFSDLQESVQTALPYAQATGVKRL
                     HLMAGIADRGNRQAVEAFTKSVVWAAEFLKPHGLDVVIEPINPRNVPGYFLNDFSFAR
                     DLIVELKIPNLKLQFDIYHCQIIHGDVTMRLREMMPIIGHIQIASIPSRHEPDDEELN
                     YPFLFEELDRLDYGGFVGCEYNPRGRTIDGLGWFKSYAGVKP"
     gene            complement(3027..3947)
                     /gene="ltnD"
                     /locus_tag="VGA15_10885"
     CDS             complement(3027..3947)
                     /gene="ltnD"
                     /locus_tag="VGA15_10885"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HEX9588233.1"
                     /translation="MPESISAKPRVAVIGLGSMGFGMATSLRRAGFDVTGCDVSADAV
                     ARFVADGGGGANTPAEAAKAADIVVSVVVNAAQTEAVLFGKDGAAETLAGDAVFVSSA
                     TMDPDVARRLARQLEASGRHYLDAPISGGAQRAAQGELTILASGSAAAFAKARPALDA
                     MAAKLYELGDAAGQGAAFKMINQLLAGVHIAAASEAITFAAKQGLDIRKVYEVITASA
                     GNSWMFENRMPHVLDGDYTPRSAVEIFVKDLGIIQDMARTARFPVPVAAAALQMFLMT
                     AASGMGRDDDASVARIYARVTGTHLPGDPE"
     gene            4074..4736
                     /locus_tag="VGA15_10890"
     CDS             4074..4736
                     /locus_tag="VGA15_10890"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX9588234.1"
                     /translation="MSESKIREEICRLGRSLFERGLTPGSSGNISVGLDDGGWLVTPT
                     NASLGSLDPARLSRLGADGRLLSGDAPTKEVPLHTALYQTRRTARAVVHLHSTHSVAL
                     SMLPEIDPRAALPPMTAYYLMKCGATALVPYYRPGDPAVADAIKGFAGKYASVLLANH
                     GPVVAGETLEAAVFAIEELEETAKLYLLLRGLNPRYLTPAQVADLSKTFGLTLPEHGH
                     EH"
     gene            complement(4888..5592)
                     /locus_tag="VGA15_10895"
     CDS             complement(4888..5592)
                     /locus_tag="VGA15_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588235.1"
                     /translation="MLSVHEVTTAYQGLVAISAVSIEVAKGEIVCVAGANGAGKSTLL
                     KSIAGAERPRSGTVTFDGARIDGMAQHLVTARGIAYVPENRRLFPRLSVRDNLRLGSY
                     LYRGETYRDAPLDLVFKLFPRLSERLDQRAETLSGGEQQILAIGRALMTRPRLLMLDE
                     PSQGIMPKLVDEIFAAVKEIRDAGMTVLIVEQRMSECLEIADRAYILQTGRVLMQGAA
                     SDIMGNPDVRKAYLGL"
     gene            complement(5576..6325)
                     /locus_tag="VGA15_10900"
     CDS             complement(5576..6325)
                     /locus_tag="VGA15_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588236.1"
                     /translation="MTPLLETQSLLEIHGVWQRFGGLIANSDISISVGRGEIVGLIGP
                     NGAGKSTLFNLIAGVLPPTQGSIWFDGEDVTALPAAERCQRGIARTFQVVKSFETMTV
                     IDNVIVGALVRTTVMREARRKAYEVLEFAGLAGRADVSAGELIPSEKRRLEVARALAT
                     EPKLLLLDEVLTGLTPIEAQTGVELVRKVRATGVTVLMVEHVMEIVMPLVDRAIVLDL
                     GKVLVEGKPADIVRDPKVISAYLGDRHAVGA"
     gene            complement(6322..7302)
                     /locus_tag="VGA15_10905"
     CDS             complement(6322..7302)
                     /locus_tag="VGA15_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588237.1"
                     /translation="MDKTFAQRRRRDLIVAVCLAVIAALVPLFVKDVYVQNIMVLTLM
                     YAALSQSWNILGGYCGQISLGHALYFGLGAYTTALLFTKFGVLPWFGMLGGGLISASI
                     AMALGYPCFRLRGHYFVIATIVIAEIALLLFQNWNWAGAALGIDIPVRGDSWLKFQFT
                     RSKLPYFYFALALACVAWLVTWWLEDSKWGYWWRAVKDNPDAAESLGVVVFNSKMGAA
                     AVSAFLTAVGGSFYAQFVSYIDPESVMGFQFSLLMALPAVLGGIGTLWGPVLGAVILI
                     PLTELTRSFIGGSGRGVDLIVYGTLIVLISLARPEGLVGLFSRRRKGVAR"
     gene            complement(7302..8180)
                     /locus_tag="VGA15_10910"
     CDS             complement(7302..8180)
                     /locus_tag="VGA15_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588238.1"
                     /translation="MTAETIIQSLASGLLMGLLYGLIAVGLALIFGLMDVVNFAHGEF
                     LMIAMYATFFLFAFFAVDPLLAAPLVAAALFVFGAVVYLLIVRFAVRAKANAGMVQIF
                     STFGLAIVMRGLAQYFFTPDYRSITHSWLGGKTVSVAGIFLPVPQLAGALVSIAAFGA
                     LYFFINLTDFGRALEATREDAGAVALVGIDKNRVFALGWGLGAALVGLAGAIMAMFFY
                     IYPDVGASFALIAYVTVALGGFGSVFGAFAGGIIVGLVEATTALILPPSLKSVGIYAV
                     YLLVVFIRPRGLFGSL"
     gene            complement(8186..>9328)
                     /locus_tag="VGA15_10915"
     CDS             complement(8186..>9328)
                     /locus_tag="VGA15_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588239.1"
                     /translation="TYPFSGANAQIGVDAQKAYETAAEIINKNYDFDLLLAKGEGLSG
                     LGGAKVRLVFADHQSDPQKGRAETERLITQEKVCAVIGTYQSAVAVTVSQICERYQIP
                     FISADNSSPSLHRRGLKFYFRAAPHDEMYSAAMFDFFDAMKKKGTKIETLALFHEDTI
                     FGTDSGNAQLKLANERGYKVVADIKYRSNSPSLSAEVQQLKAANADVLMPSSYTTDSI
                     LLVKTLAELGYKPNAIVAQDAGFSEKALYDAVGDKLEGVISRGTFSLDLATKRPMVGK
                     FNAMFKEKSGKDFNDLTSRQFMGLIVMADAISRAKSTDGDKIREALVATDIPGEATIM
                     PWKRIKFDEMGQNNDADPVLLQYVGGKFVTISPPQAAVAEATWPMK"
BASE COUNT         1782 a         2979 c         2950 g         1617 t
ORIGIN      
        1 gtctcaccct gatgcggcac ctgcgcggtc aggtcgccgc tgcccagcgc ctgcatcggc
       61 gcaacaatgg acctgattcc agtggagacg tcgcgaacca gcaggatcgc agcgcctatt
      121 cccaggataa ttgccagtag cagcgacgcc agcaccatct tgatggcctc gtcggagctt
      181 tgctcggcct ggctgcctgc cgcatccgcg cccttgttgt tcagcaggac gtccttttgc
      241 agcgtctcgt cggcatggac gccggcaagc gacgccttgg catggagtag cctcgcctcc
      301 ttgtcctcat tcttgcggga cagcgccagg acctccttta cgccggcgag atattcggcc
      361 caggatgtgc tgaactcgtc gtacaatacg cgttcctcgg ccgaggtgat caaaggttca
      421 taggctttgc gggccttgtc ggtgttgttg atccacttgg cgagcagatc ctcctgaacc
      481 tgcctcgtgg cctcttcggt agccagcaga tgggagcgga cgatcgcccg ataggtgatg
      541 gtcgcggccc gcaattcacc gaggacgcgc acgctcggca gccaactggt cgtgatgtcg
      601 atggtggagg aattgatcgc atacatctgc cggatgttca gcgctcccat taacgacatg
      661 gcgacgagca gaaaagcgac gacagccgta atcttggtgc gaatggagta tttggcgagc
      721 atgaacggaa tctctcggct ttgagcctgt tggcgcttcg gtcgtgaacc acgggattcg
      781 gcaggagcgg aggccatgcc tgccggaatc gagccggagc gtatcgcgct tccgttaata
      841 attttccgta ggactacgga gaccgacggg cgccgacaga tgaagccggg cgcgagctca
      901 gggcatcagc ttcagggcat cggagaagaa ttccgggccg ccgaaattgc ccgatttcag
      961 cgccagcagc atcgcgcctt ccttcgcgcc gacggcacgc agcaccggca cgccggcggc
     1021 gatttccgcg cccaccagaa agccgggaat ggtcaggcga tccaccaccg caccggaggt
     1081 ttcaccgccg gcaaccacca gccgccggac gccggactgc accaggcctt gcgcgatatc
     1141 ggccattgcc tgctcgatcg cgtggcccgc ggcctcgcgg ccgtggcgcg cctgcagcgc
     1201 cgcgaccggg tccggcgtcg cactgctggc gatcaggacc ggacctttac cgagatgttc
     1261 attggcccag ccaagtgcgc gctgcacctc ggctttgccc gcgaccgcct gttcggaatc
     1321 aagatgaaac accggcatga cccgctcggc gtttgcgatc tgctgcagcg tcgcctgcga
     1381 acaactgccg gccaggcacg ccgccggtcc gccgaccgca gcgtcggcca tcgcgtcagg
     1441 cgcattcgat ttgacccggc ttgaggccac cagcgcgcgg gcaagaccaa gcccgatacc
     1501 ggatgcgccc acggagagcc gatgatcgag cgccacgacg ccgatggttt cgagatcgcg
     1561 ggcaaatacc gcgtccgcaa tcgctgcacc aaaacccctg gtggaaagat cagccagatg
     1621 cgcgcggacc gcatcgggcc cgcgcgcgat gaccgcgagg tcgacgaggc cgactttggt
     1681 cttgctctgc cgcgccagca cccgcagcag attggaatca tgcatcggat tgagcggatg
     1741 atcctttagg gggctctcgt tgagcggcac cgagccgacg aacagattgc cctgatagac
     1801 ggtgcggccg gtttcgggaa acgccggcgt caccagcacg attgtctcgc cgcaatcggc
     1861 gcgcagcgca tccatcaccg ggccgatatt gccggcgtcg gtggaatcga aggtcgagca
     1921 gatcttgaac agcacatgcg gcgcaccccg cccgcgcagc catttttccg cggcgcgcga
     1981 agcggtcacg gcgaggccgg cgtcgatcga ccggcttttc agcgacacca ccacggcatc
     2041 gacttcaggc agcgcgagat cgcttaaggg cacgccgatg gtctgcaccg tgcgcaggcc
     2101 gcagcgggtc agcgtattgg cgagatcgga cgcgccggtg tagtcgtcgg cgatgcagcc
     2161 cagcttaagc gtcacggttt tactccggca taggacttga accagccgag gccgtcgatg
     2221 gtcctgcccc gcgggttata ttcgcagccg acaaaaccgc catagtcgag ccggtccagt
     2281 tcctcgaaca ggaacggata gttcaattct tcgtcgtcgg gctcgtgacg cgagggaatg
     2341 ctggcgatct ggatgtggcc gatgatcggc atcatctcgc gcagccgcat ggtcacgtca
     2401 ccgtggatga tctggcaatg atagatgtcg aactgcagtt tcaggttggg gattttcagc
     2461 tccacgatca ggtcgcgggc gaatgaaaaa tcgttgagga agtagccggg cacgttgcgc
     2521 ggattgatcg gctcgatcac gacgtcgagg ccatggggct taagaaattc ggcggcccat
     2581 accaccgatt tggtgaacgc ctcgaccgcc tgccgattgc ctctgtcggc aatccccgcc
     2641 atcaggtgca ggcgcttgac gccggtcgcc tgcgcatagg gcagcgccgt ctgaacgctc
     2701 tcttgcagat cggaaaaccg acccggcaac gcggcaaagc ctttttcgcc ggcttcccag
     2761 ttgccgggcg gcaggttgaa caacgcctgg gtcaggccgt tgcgatgcag ccgctcgccg
     2821 acggcctccg ccggatggtc atagggaaac aggaattcca cggccgtgaa gcccgctctt
     2881 gccgccgcct cgaaacggtc gaggaacgga acttcagtga acatcatgct gagattggcg
     2941 gcaaaacgcg gcatcgacgg tcctcttgcg ggttcacggt tatttcggct cgctgctatt
     3001 cggctcgctc ttatttagga tcactcttat tccggatcac ccggcagatg cgtgccggtg
     3061 acgcgggcat atatccgcgc caccgaggcg tcgtcgtcgc ggcccatgcc ggaggccgcc
     3121 gtcatcagaa acatctgcag cgcggccgct gccaccggca ccggaaatct cgccgtgcgc
     3181 gccatgtcct ggatgatgcc gaggtccttg acaaagatct ccacagcact tcgcggggtg
     3241 taatcgccgt cgagcacatg cggcatgcgg ttctcgaaca tccaggaatt gccggcggac
     3301 gcggtgatga cctcgtagac cttgcggata tcgaggccct gtttggcggc aaatgttatc
     3361 gcttccgatg cggcggcaat atgaacgccg gccagcaact ggttgatcat cttgaaggca
     3421 gcgccctgcc ctgcggcatc gccgagttcg tagagcttgg ccgccatcgc gtcgagcgcg
     3481 ggacgcgcct tggcaaacgc ggcagcgctt cccgatgcga gaatggtcag ttcgccctgc
     3541 gccgcacgct gggcgccgcc agagatcggt gcgtcaagat aatgccggcc gctggcctcg
     3601 agctgccttg ccagccgccg cgcgacatcc gggtccatcg tggcggagga cacgaacacg
     3661 gcatccccgg ccagggtttc ggcagcgccg tccttgccga acagaacggc ttcggtctgc
     3721 gcggcgttga cgaccacgct gacgacgatg tcggctgcct tcgcggcctc ggccggtgtg
     3781 tttgcacccc ctccgccgtc agcgacgaac cgcgccacgg catcggccga gacgtcgcag
     3841 ccggtcacgt caaaacccgc gcgccgcagc gaggtcgcca tgccaaaccc catcgagccc
     3901 agcccgatga cggcgacgcg cggttttgcg gaaatggatt caggcataag gcattacctc
     3961 aggtttcctc acggccgaat tcaacaacgg cttggcctct gggtatcacg gcttggccgc
     4021 gctgccaaag catgagacaa ggcgggaaac aggtatgccg ccgggatact gccatgagcg
     4081 aaagcaaaat ccgtgaggaa atctgccgcc tcggccgctc gctgttcgag cgcgggctga
     4141 cgccgggttc ctcgggcaat atcagcgtcg gacttgatga cggcggctgg ctggtgacgc
     4201 cgaccaatgc gtcgctcggc tcattggacc cggcgcggct gtcgcgcctc ggcgctgatg
     4261 gccgcctcct ctccggcgat gccccgacca aggaagtgcc gctgcatacc gcattgtacc
     4321 agacgcgccg caccgcgcgc gcggtggtgc acctgcattc gacccattcg gtggcgctgt
     4381 cgatgctgcc cgagatcgac ccgcgcgccg cgctgccgcc gatgacggcc tattatctga
     4441 tgaaatgcgg ggccaccgcg ctggtgccgt attatcggcc cggcgatccg gcggtggccg
     4501 acgcgatcaa gggttttgcg ggaaaatatg cttccgtgct gctcgccaat cacggcccgg
     4561 tggtggcggg cgaaacgctg gaagccgcgg tcttcgccat cgaggagctg gaagaaaccg
     4621 caaaactcta tctgctgctg cgcgggctca atccgcgtta tctgacgcca gcgcaggtgg
     4681 ccgatttgtc gaagacgttc gggctgacgc tgccggagca tgggcacgag cattgaaggc
     4741 tgcgttcccc ggatgctgcg cagcgcgccg cgcttgcggc gtggtgcgct gctgatccgg
     4801 ggcccacgct acaagcacca ccttgtcatg ggtcccggct ctgcggagca gcgtgaagaa
     4861 cgctgcaccg cgtccgggac acgatcatca caatcccaga tacgccttgc gcacgtccgg
     4921 gttgcccatg atgtcgctgg ccgcgccctg catcagcacg cggccggtct gcaggatgta
     4981 ggcgcggtcg gcgatctcga ggcattccga catgcgctgc tcgacgatca gcacggtcat
     5041 ccctgcgtcg cggatttcct tcacggcggc aaagatctcg tcgaccagtt tcggcatgat
     5101 gccctgcgac ggctcgtcca gcatcaacag ccgtggccgc gtcatcagcg cgcggccgat
     5161 cgccaaaatc tgctgctcgc cgccggaaag cgtttcggcg cgctggtcca gccgctccga
     5221 caatcgggga aacagcttga acaccagatc cagcggcgcg tcgcgatacg tctcgccgcg
     5281 atagagataa ctgccgagcc gcagattgtc gcgcaccgac agccgcggaa acagccggcg
     5341 gttttccggc acataggcga tgccgcgcgc ggtgaccaga tgctgcgcca tgccgtcgat
     5401 ccgcgcacca tcgaaggtga cggtgccgga gcgcggacgt tcggcgccgg cgatggattt
     5461 caaaagcgtc gatttgccgg cgccattggc cccggcgacg cagacgatct cgccttttgc
     5521 cacttcgatg ctgaccgccg aaatcgcgac cagcccctga taggcggtgg tgacttcatg
     5581 caccgacagc atgacgatcc ccaagatagg ccgagatgac tttcggatcg cgaacgatgt
     5641 cggcgggctt gccctcgacc agcaccttgc cgaggtcaag cacgatggcg cggtcgacca
     5701 gcggcatcac gatttccatg acgtgctcga ccatcaatac ggtgacgccg gtggcgcgca
     5761 ctttccgcac cagttcgacg ccggtctgcg cctcgatcgg cgtcaggccg gtcagcactt
     5821 catcgagcag cagcagctta ggttcggtgg cgagcgcgcg ggcgacctcg aggcgacgtt
     5881 tctccgacgg gatcaactcg ccggccgaca cgtcggcgcg gccggcaaga ccggcaaatt
     5941 ccagcacctc gtaggctttg cggcgcgcct cgcgcatgac ggtggtgcgg accagtgcgc
     6001 cgacgatgac attgtcgatc accgtcatgg tctcaaagct tttcacgacc tggaaggtgc
     6061 gcgcgatgcc gcgctggcag cgctccgccg ccggcagcgc ggtaacgtct tcgccgtcga
     6121 accaaatcga accctgggtc ggcggcagca cgcccgcgat cagattgaac aaagtcgact
     6181 tgccggcgcc gttgggcccg atcaatccga cgatctcgcc gcggccgacc gaaatcgaga
     6241 tatcgctgtt ggcgatcagg ccgccgaaac gctgccagac gccatgaatt tccagcaagg
     6301 attgggtttc cagcaagggc gtcatcgggc cactcccttg cgccgccgcg aaaacagtcc
     6361 gaccagacct tcgggccgcg ccagcgagat cagcacgatc aacgtgccgt agacgatcag
     6421 atcgacgccg cggccggagc cgccaatgaa ggagcgcgtc agttccgtga gcgggatcag
     6481 gatgacggcg ccgagcactg gtccccacag cgtgccgatg ccgccgagca ccgccggcag
     6541 cgccatcaat agcgagaact ggaaacccat cacgctttcc ggatcgatgt aggacacgaa
     6601 ctgcgcgtaa aaactgccgc cgaccgcggt gaggaaggcc gagaccgccg ccgcccccat
     6661 cttggaattg aacacgacca cgccgagact ttcggcggca tccggattgt ccttcacggc
     6721 gcgccaccaa taaccccatt tggaatcttc cagccaccag gtcacgagcc aggcgacgca
     6781 ggccagcgcc agcgcgaaat agaagtatgg caatttgctg cgggtgaatt ggaatttgag
     6841 ccagctgtcg ccacgcacgg gaatatcgat gccgagtgcc gcgccggccc aattccagtt
     6901 ctggaacaac agcaaagcga tctcggcaat gacaatggtg gcgatgacga aataatgccc
     6961 gcgcaggcgg aaacagggat agccgagcgc catcgcaatg cttgccgaga tcaggccgcc
     7021 gccgagcatg ccaaaccacg gcagcacgcc gaatttggtg aacagaaggg ccgtggtgta
     7081 ggcgccgagc ccgaaataca gcgcgtggcc gagcgagatt tgcccgcaat agccgccgag
     7141 gatattccag ctctgcgaca acgccgcgta catcagcgtc agcaccatga tgttctggac
     7201 gtagacgtct ttcacgaaca gcggcaccag tgctgcgatc accgccaggc acacggccac
     7261 gatcagatcg cggcggcggc gctgggcaaa ggtcttgtcc atcagagcga tccgaacagg
     7321 ccgcgcggcc ggatgaagac caccagcaga tagaccgcgt agatgccgac cgatttcagc
     7381 gacggcggca ggatcagtgc cgtggtcgct tcgacgagcc ccacgataat gccgccggca
     7441 aaggcgccga acacgctgcc gaaaccgccg agcgccaccg tgacataggc gatcagcgcg
     7501 aacgacgcgc cgacatcggg atagatgtag aagaacatcg ccatgatggc gccggcgagc
     7561 cccaccagcg cagcacccag gccccagccc agcgcaaaca ccctgttctt gtcgatgccg
     7621 accagggcga ccgcgcccgc gtcttcacgg gtcgcttcaa gggcacggcc gaagtcggtg
     7681 agattgatga agaaatacag cgcgccgaac gccgcgatcg acaccagcgc gccggccaat
     7741 tgcggcacgg gtaagaagat gccggcgacc gagacggttt tgccgccgag ccatgaatgg
     7801 gtgatgctgc gataatccgg ggtgaagaag tactgcgcga ggccgcgcat gacgatggcg
     7861 agcccgaacg tcgagaaaat ctgcaccatg ccggcattgg ctttcgcccg gaccgcgaag
     7921 cgcacgatca ggagatagac caccgcaccg aatacaaaca gcgccgccgc gaccaagggc
     7981 gctgccagca gcggatcgac cgcaaagaac gcgaacagaa agaacgtcgc gtacatcgcg
     8041 atcatcagga actcgccatg ggcgaagttc accacgtcca tcaggccgaa gatgagcgcc
     8101 agccccacgg cgatcaggcc gtagagcagc cccatcaaga ggcctgaggc aagactctgg
     8161 atgatggtct cggccgtcac ggcccttact tcatcggcca ggtggcttcg gcgacggcgg
     8221 cttgcggcgg ggagatggtg acgaacttgc cgccgacata ttgcagcagc accggatcgg
     8281 cgtcgttgtt ctggcccatc tcgtcgaact tgatgcgctt ccacggcatg atggtggcct
     8341 cgcccgggat gtcggtggcg accagcgcct cgcggatctt gtcgccgtcg gtggacttgg
     8401 cgcggctgat cgcatccgcc atcacgatca ggcccatgaa ctgccgcgac gtgaggtcgt
     8461 tgaaatcctt gccggacttt tccttgaaca tggcgttgaa cttgccgacc atcggccgct
     8521 tggtggcgag atcgagcgaa aaggtgccgc gcgagatcac gccttcgagc ttgtcgccga
     8581 ccgcatcata cagggctttt tccgagaagc cggcgtcctg cgcgacaatg gcgttcggct
     8641 tgtagccgag ctccgccagg gtcttcacca gcaggatgct gtcagtggtg tagctcgagg
     8701 gcatcagcac gtcggcgttg gctgccttga gctgctgcac ctccgccgac aatgacggcg
     8761 aattggagcg gtatttgata tcggcgacga ctttgtagcc gcgctcattg gcgagtttca
     8821 gttgcgcgtt gccggaatcg gtcccgaaga tagtgtcctc gtggaacagc gccagcgtct
     8881 cgatcttggt gcctttcttc ttcatggcat cgaagaaatc gaacatcgcg gccgagtaca
     8941 tttcgtcgtg gggtgctgcg cggaaataaa atttcaggcc gcggcgatgc aggctcggcg
     9001 aggaattgtc cgccgagatg aagggaatct gatagcgctc gcagatctgg ctgacggtga
     9061 ccgcgaccgc gctctgatag gtgccgatga cggcgcagac cttttcctgc gtgatcaggc
     9121 gttcggtctc ggcacggccc ttttgcggat cggattggtg gtcggcgaac acgaggcgga
     9181 ccttggcgcc gccgaggccg gacaaacctt cgccctttgc cagcagcaga tcgaaatcgt
     9241 agttcttgtt gatgatctct gccgcggtct cgtacgcctt ctgggcatcg acaccgattt
     9301 gtgcgttggc gccggagaac ggataggt
//
LOCUS       DASWGM010000374         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1978119,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000374 DASWGM010000000
VERSION     DASWGM010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978119"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2322
                     /locus_tag="VGA15_10920"
     CDS             <1..>2322
                     /locus_tag="VGA15_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9588240.1"
                     /translation="MTGDGADYVQLKKAAEGMRQRLLKVPGVTKVDLYGTQDERIYVE
                     FSHAKLATLGITPQALFDSLAKQNNVVPAGTVETASQRVPLRVTGALDGVKAVAETPV
                     ESNGRVFRLGDIATVTHGFVDPPTFVARQEGKPALGIGVVTAKGANILELGKDVANAT
                     AEFMQAVPQGINVELIADQPKVVEHAVTEFLHSFIEALAIVLFVSFVALGWRTGIVVA
                     ASVPLVLAIVFIVMNAMSLDLHRITLGALIIALGLLVDDAIIAVEMMVVKMEQGWDRV
                     RAASFAWESTAFPMLTGTLVTAVGFLPIGFANSAVGEYAGGIFWIVAIALVASWFVAV
                     IFTPYIGVKLLPNITVSHNHDPHAVYETRFYRILRSMIQWCVDHRVKVVLATAGVFAL
                     SIVGFGHVQQQFFPLSERPELFLQLRLPEGTAFGVTEQSVKKAEALLKDDKDIATYTA
                     YVGQGSPRFWLGLNPQLPNESFAEIVVVARDVEARERIKARLEKAVNDGALTEARVRV
                     DRFNFGPPVGFPVQFRVIGPDANTVRDIAYKVRDVVRQNDKVKDPQLDWNEQSPYLKL
                     VVDQDRARALGLTPQDVSQALSMLISGAPVTTIRDGIEKVGVVARAVPSERLDLGHVG
                     DLTITSRNGVAVPLTQVAKIEYAHEEPIIWRRNRDMAITVRGDVVDGVQAPDVTSQIW
                     PKLQEIRDSLQPAYRIEAGGAFEESAKGNASIFVLFPLMVVVMLTLLMIQLQSFSRLL
                     LVFLTAPLGIIGASLGLNVANQPFGFVALLGLIA"
BASE COUNT          401 a          727 c          749 g          445 t
ORIGIN      
        1 gatgaccggc gatggcgccg actacgtgca gctcaagaag gcagctgaag gtatgcgtca
       61 gcgcctgttg aaggtaccgg gagtgacaaa agtcgatctc tacggcaccc aggacgagcg
      121 aatctatgtc gagttctcgc atgccaagct ggcgacgctg ggaattacgc ctcaggcgct
      181 gtttgattcg ctggccaagc agaacaacgt cgtgccggcc ggcacggtcg aaaccgcctc
      241 acaacgcgtg ccgctgcgcg tcaccggcgc gctcgatggc gtcaaggccg tcgcggagac
      301 accggtcgaa agcaatggcc gcgtgttccg gcttggcgat atcgcgactg tgacacacgg
      361 cttcgtcgac ccgccgactt ttgtggcgcg tcaggaaggc aaacccgcgc tcggcatcgg
      421 cgtggtcacg gccaagggcg ccaatattct ggaactcggc aaggacgttg ccaatgcaac
      481 cgcggaattc atgcaggcgg tgccgcaagg catcaatgtc gaactgatcg ccgatcagcc
      541 caaggtggtc gagcacgccg tcactgaatt cttgcactcc ttcatcgagg cgctggcgat
      601 cgtgctgttc gtcagtttcg tggcgctggg ctggcgcacc ggcatcgtgg tggcggcgtc
      661 ggtgccgctg gtgctggcga tcgtctttat cgtcatgaat gcgatgtcgc tcgacctgca
      721 tcggatcacg ctcggcgcgc tgatcatcgc gcttggacta ttggtcgacg acgccatcat
      781 cgcggtcgag atgatggtgg tgaagatgga gcagggctgg gaccgcgtcc gcgcggcgtc
      841 ctttgcctgg gaatccacag cgtttccgat gctgacggga accctggtca cggccgttgg
      901 tttcctcccc atcggctttg ccaattcggc ggtcggtgaa tacgccggcg gcattttctg
      961 gatcgtcgcc atcgcgctgg tcgcgtcatg gttcgtcgcg gtgatcttta cgccctatat
     1021 cggcgtcaag ctgctgccga acatcacggt cagccacaac cacgatccgc atgcggtcta
     1081 tgaaacgcgc ttctaccgca ttctccgcag catgattcaa tggtgcgtcg accaccgggt
     1141 caaggtggtg ctggcaacgg cgggcgtctt cgcgctctcc atcgttggct tcggtcacgt
     1201 ccagcagcag ttcttcccgc tgtccgagcg ccctgaactg ttcctgcaac ttcggctgcc
     1261 cgaaggcacg gcgttcggcg tcaccgagca gtccgtgaaa aaggcggaag ccctgctgaa
     1321 ggacgacaag gacatcgcga cctacaccgc ctatgtcggc caggggtcgc cgcgcttctg
     1381 gctcgggctc aatccgcagc tgccgaacga atccttcgcc gaaatcgtcg tcgtcgccag
     1441 ggatgtcgag gcgcgtgaac ggatcaaggc gcggctggag aaggccgtca atgatggtgc
     1501 gttgaccgaa gcgcgggtgc gcgtcgaccg cttcaacttc ggcccgccgg tcggctttcc
     1561 ggtgcagttc cgcgtgatcg gccccgacgc caacaccgtc cgcgacatcg cctacaaggt
     1621 gcgcgatgtg gtcaggcaga acgacaaggt caaggatccg cagctcgact ggaacgagca
     1681 gtcgccctac ctgaagctcg tggtcgatca ggatcgcgcc cgcgcgctcg gcctcacccc
     1741 gcaggatgta tcgcaggcgc tgagcatgct gatttcgggc gcgccggtga cgacgatacg
     1801 cgacggcatc gagaaggtcg gcgtggtcgc gcgcgcggtt ccgtcggagc ggctcgatct
     1861 cggccatgtc ggcgatctca ccatcacctc gcgcaacggc gtcgcggtgc cgctgacgca
     1921 ggtcgccaaa atcgaatacg cccacgagga gccgatcatt tggcggcgca accgtgacat
     1981 ggcgatcacg gtgcgcggcg acgtcgtcga cggcgtgcag gcgcccgacg tgaccagcca
     2041 gatctggccc aagctgcagg aaattcgcga cagcctgcag ccggcctacc ggatcgaggc
     2101 ggggggcgcg ttcgaggagt ccgccaaggg caacgcgtcg atcttcgtgc tgtttccgtt
     2161 gatggttgtc gtcatgctga ccttgctgat gatccagctg cagagttttt cgcgcctgct
     2221 gctggtgttt ctcaccgcac cgctcggcat catcggcgca tcgctcggac tgaacgtcgc
     2281 caaccagccg ttcggcttcg tggcgctgct cggactgatc gc
//
LOCUS       DASWGM010000375         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_996009,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000375 DASWGM010000000
VERSION     DASWGM010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996009"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..>2731)
                     /gene="carB"
                     /locus_tag="VGA15_10925"
     CDS             complement(17..>2731)
                     /gene="carB"
                     /locus_tag="VGA15_10925"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX9588241.1"
                     /translation="TDEVLIEESVLGWKEFEMEVVRDKKDNCIIICSIENLDPMGVHT
                     GDSITVAPALTLTDKEYQIMRDASLAVLREIGVETGGSNVQFGVNPADGRMVVIEMNP
                     RVSRSSALASKATGFPIAKVAAKLAVGYTLDEIANDITGGATPASFEPTIDYVVTKIP
                     RFAFEKFPGASTTLTTSMKSVGEVMAIGRTFQESLQKALRGLETGLTGLDEIEIEGLG
                     RSDDKNAIRAALGTPTPDRLLQVAQAMRLGWSNDEIFTSCKIDPWFLAEMRGIVEMES
                     KIKKSGLPGNAFGMRTLKAMGFSDARLAVLTKTTEAEVTAQRRALGVRPVFKRIDTCA
                     AEFASPTAYMYSTYEAPFAGALADESAPSDKDKVIILGGGPNRIGQGIEFDYCCCHAC
                     FALHDAGYETIMINCNPETVSTDYDTADRLYFEPLTSEDVLEIIATERQNGTLHGVIV
                     QFGGQTPLKLAHALEAADVPILGTSPDAIDLAEDRDRFKRVLDKLRLKQPKNGIAYSV
                     EQARLVAADLGLPLVVRPSYVLGGRAMQIIREETQLGDYLLGTLPELVPGDVKARYPN
                     DKTGQINTVLGTNPLLFDRYLSDAIEIDVDCLCDGKDTFIVGIMEHIEEAGIHSGDSA
                     CSLPPHSLDAQMIGELERQTRELALGLDVVGLMNVQYAIKDGDIYVLEVNPRASRTVP
                     FVAKVMGMPVAKIAARIMAGEKLADFNLKKRKLGHVGVKESVFPFARFPGVDTVLGPE
                     MRSTGEVMGIDRSFEVAFAKSQLGGGTRVPRKGTVFVSVREIDKTRIVDAVRLLFSLG
                     FKVMATSGTQRFLADRGVPTEKVNKVLEGRPHIVDAITNGDIQLVFNTTEGPQALADS
                     RSLRRAALLHKVPYYTTLSGAVAAAQGIRAYLGGDLEVRTLQSYFSET"
BASE COUNT          485 a          847 c          872 g          527 t
ORIGIN      
        1 cggtccggcc aaaagatcag gtttcggaaa agtaactctg caaggtgcgg acctcaaggt
       61 ccccgcccag ataggcgcgg ataccctgcg cggccgccac ggcacctgaa agagtggtgt
      121 aatatggcac tttatgcaag agggcagctc gccttaacga gcggctgtcg gccagggcct
      181 gcggcccttc ggtggtgttg aaaaccagct ggatgtcgcc attcgtgatg gcatcgacga
      241 tatgcggccg gccttccagc accttgttaa ccttttcggt cggcacgccg cgatcggcca
      301 ggaaacgctg cgtgcccgag gtggccatca ccttgaagcc aagcgagaat agcagccgca
      361 ccgcatccac gatccgggtc ttgtcgatct cgcggacgga gacgaatacg gtgcccttgc
      421 gcggcacgcg ggtgccgccg ccaagctggc tcttggcgaa agcgacctcg aacgagcggt
      481 cgatccccat cacctcgccg gtcgatcgca tctccggacc gagcacggta tcgacgccgg
      541 ggaaacgggc gaacggaaac accgactcct tgacgccgac atggccgagc ttgcgcttct
      601 tcaaattgaa gtcggcgagc ttttctccgg ccatgatcct ggccgcgatc ttggccaccg
      661 gcatgcccat caccttggcg acgaacggca cggtgcgcga cgcccgcgga ttgacttcga
      721 gcacgtagat gtcgccgtcc ttgatggcgt actgcacgtt catcagtccg accacgtcga
      781 ggccgagcgc caactcacgc gtttgccgct ccagttcgcc gatcatctgg gcgtcgagcg
      841 aatgcggcgg cagcgagcag gcggaatcgc cggagtgaat gccggcttcc tcgatgtgct
      901 ccatgatgcc gacgatgaag gtgtccttgc cgtcgcacag gcagtcgaca tcgatctcga
      961 tggcgtcgga cagataacgg tcgaacagca gcggattggt gccgagcacg gtgttgatct
     1021 ggccggtctt gtcgttcgga taacgcgcct tgacgtcgcc cggcaccagc tccggcaagg
     1081 tgccgagcag gtaatcgccg agctgggttt cctcgcggat gatctgcatc gcgcggccgc
     1141 cgagcacata ggacggacgc accaccagcg gcagcccgag atcggcggcc accagccgcg
     1201 cctgttcgac cgaataggcg ataccgtttt tcggctgctt caagcgcagc ttgtcgagca
     1261 cgcgcttgaa gcggtcgcgg tcttcggcca gatcgatggc gtcgggcgag gtgccgagga
     1321 tcggcacatc ggccgcctcc agcgcgtggg cgagtttcag cggcgtctga ccgccgaact
     1381 gcacgatcac gccgtgcaag gtgccgttct ggcgctcggt ggcgatgatt tcgagcacgt
     1441 cctccgatgt caggggctcg aaataaagcc ggtcggcggt atcgtagtcg gtcgataccg
     1501 tctccggatt gcagttgatc atgatggttt catagccggc gtcgtgcagt gcaaagcagg
     1561 catggcagca gcaatagtcg aactcgatgc cttgcccgat ccggttggga ccgccgccga
     1621 gaataatcac cttgtccttg tcggaggggg cgctctcgtc ggcgagcgcg ccggcgaatg
     1681 gcgcttcata ggtcgaatac atgtaggcgg tgggcgaggc gaactcggcg gcacaggtgt
     1741 cgatgcgctt gaacaccggc cgaaccccga gcgcgcggcg ctgtgccgtc acttcggcct
     1801 cggtggtttt ggtcaggacc gcaagccgcg catcggaaaa gcccatcgcc ttcaacgtcc
     1861 gcatcccgaa tgcgttgccg ggcaggccac ttttcttgat cttggattcc atttcgacga
     1921 tgccgcgcat ttcagccaga aaccacgggt cgatcttgca ggaggtgaag atctcgtcat
     1981 tgctccagcc gagccgcatc gcttgggcta cctgcaacag ccgatccggc gtcggcgtgc
     2041 ccaatgcggc acggatcgcg ttcttgtcgt cgctgcggcc gaggccctcg atttcgattt
     2101 cgtcgagccc ggtcaacccg gtttcgaggc cgcgcaaggc tttctgcagg ctttcctgaa
     2161 aggtgcggcc gatcgccatc acttcgccga ccgacttcat cgaggtggtc agcgtggtcg
     2221 aggcgccggg aaatttctcg aacgcaaagc gcgggatttt ggtgacgacg taatcgatgg
     2281 tcggctcgaa cgaggcgggg gtggcgccgc cggtgatgtc gttggcgatc tcgtcgaggg
     2341 tgtagccgac cgccagcttg gcggcgactt ttgcgattgg aaagccggtg gcctttgacg
     2401 cgagcgcgga cgaccgcgat acccgcggat tcatctcgat cacgaccatg cggccgtcgg
     2461 ccggattgac gccgaactgc acgttggagc cgccggtctc gaccccgatc tcgcgcagca
     2521 ccgccagcga ggcgtcgcgc atgatctggt attccttgtc ggtcagggtc agcgccggcg
     2581 cgaccgtgat ggaatcgccg gtatgcacgc ccatcggatc gaggttctcg atcgaacaga
     2641 tgatgatgca attgtctttt ttgtcgcgca ccacctccat ctcgaattct ttccacccca
     2701 gcaccgattc ctcgatcagg acctcgtcgg t
//
LOCUS       DASWGM010000376         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1142980,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000376 DASWGM010000000
VERSION     DASWGM010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142980"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGA15_10930"
     CDS             complement(<1..527)
                     /locus_tag="VGA15_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003554581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588242.1"
                     /translation="MLDWLGTIIQGVLLGGLYALFAAGLSLIFGIMRLVNLAHGDLIV
                     LAAFAILSLATWLGFDPFLATLLAMPAMFAAGFALQHVLLNRALGRDLLPPLLVTFGL
                     SIIIQNGLLEVFTADSRRLRLGAIETASIELGGNLAIGVMPLLTLGSAIAVVLLLNLL
                     FYRTALGRAFRATSD"
     gene            complement(530..1225)
                     /locus_tag="VGA15_10935"
     CDS             complement(530..1225)
                     /locus_tag="VGA15_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011648892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588243.1"
                     /translation="MLETRGLTAHYGDFQALYGIDTVIGPGETIAIIGANGAGKSSYL
                     KAITGLIRAESDSVRFGDRPIGALGAASILKLGIAMVPEGRRLFSSLTVEENLLIGSY
                     GRNVAGIWSLDSIYRLFPPLKERRTAPSHTLSGGEQQMTAIGRALMSNPRVLLCDEIS
                     LGLAPIVIRNIYDAMPRIKQTGTSIVLVEQDIAQAMKVADRVYCFQEGRVSLSGRPEE
                     LTREQVHLAYFGH"
     gene            complement(1234..>1883)
                     /locus_tag="VGA15_10940"
     CDS             complement(1234..>1883)
                     /locus_tag="VGA15_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9588244.1"
                     /translation="SVAPGEALGIIGPNGAGKTTLFNLIAGGLAPDAGEIRLNGRNIT
                     AAKPHQRCIAGIGRSYQIPRPFENLTVFENLLVAAVHGGGLAERAAAAPCAEILERLH
                     LLRRANTLAGALTLLERKRLEMARALATNPHLLLLDEIAGGLTEGECLELIETIKQVH
                     AAGMTIIWIEHIVHALVAVVGKLSVLNFGKKVAEGDPQEVMASADVRQFYIGLLA"
BASE COUNT          392 a          599 c          563 g          329 t
ORIGIN      
        1 tcgtcggacg tcgcgcgaaa cgcccggccc aacgcggtgc ggtaaaacaa cagattgagc
       61 agcagcacca ccgcgatggc agagccaagc gtgagcagcg gcatgactcc gatcgccagg
      121 ttgccgccga gctcaattga agcagtctcg atcgcgccga gccgcagccg tcggctgtcg
      181 gcggtaaaca cctcgagcaa gccgttttga atgataatgg acaagccgaa tgtgaccagc
      241 aacggcggca acagatcgcg tcccaaggct cgattcagca acacgtgctg caaggcaaat
      301 ccggcggcga acatcgccgg cattgccaac agagtcgcaa gaaaagggtc gaaaccaagc
      361 cacgtcgcca gcgagagaat cgcgaacgcc gccaacacga tcagatcgcc atgggcgaga
      421 ttgacaagcc gcatgatgcc gaagatcagc gacaggccgg cggcgaacag cgcatacagg
      481 cctccgagca gaacgccctg gatgatcgtg ccgagccaat cgagcatgct cagtgtccga
      541 aataggcgag gtgaacctgt tcgcgggtaa gttcttctgg cctgcccgac agcgataccc
      601 tgccctcctg aaagcaataa acccgatcgg cgactttcat cgcctgcgcg atgtcctgct
      661 cgacgagcac gatgctggtc ccggtctgtt tgatgcgcgg catcgcatcg tagatatttc
      721 ggattacaat cggcgccagg ccaaggctga tctcatcgca tagcagcacg cgcgggttcg
      781 acatcaacgc gcgcccgatc gcggtcatct gctgctctcc gccggatagg gtatggctcg
      841 gcgcggtgcg gcgctctttc agcggcggaa acagccggta gatgctgtcg agcgaccata
      901 tcccggcgac atttcgacca tagctcccga tcagcaggtt ttcctcgacc gtcagtgagg
      961 agaagagccg ccgcccctcc ggtaccatcg cgatcccgag cttgagaata ctcgccgcgc
     1021 ctagtgcgcc gatgggcctg tccccgaacc gcacgctatc ggactcagcg cggatcaggc
     1081 cggtgatggc cttgaggtag gaggacttgc ccgccccgtt ggcgccgatg atcgcaatcg
     1141 tttcgcctgg gccaatcaca gtgtcgatcc cgtacagcgc ctggaagtct ccgtagtgag
     1201 cggtaaggcc acgggtctcg agcatcggag gtctcacgcc aacagaccga tatagaattg
     1261 ccgaacgtcc gcggaagcca tcacctcctg cggatcaccc tcggcaactt tcttcccgaa
     1321 attcaatacg ctcagcttcc cgaccaccgc caccagcgcg tgcacgatat gctcgatcca
     1381 gataatggtc attcccgcgg catggacttg tttgatggtc tcgatcagtt cgagacactc
     1441 cccctcggtt agtcccccgg cgatttcgtc gagcagcaac aaatgggggt tggttgcgag
     1501 cgcgcgcgcc atctccagcc gcttgcgttc gagcaatgtt agcgcgccgg caagagtgtt
     1561 ggcgcgtctg agcagatgca accgctcgag aatttccgcg cagggtgcgg cggcggcgcg
     1621 ctccgccaaa cccccgccat gcaccgcggc aaccagcaga ttctcgaaca cggtgagatt
     1681 ctcgaacggc cgagggatct gatatgagcg tccgattccg gcgatgcagc gctggtgcgg
     1741 cttggccgcg gtaatattgc ggccgttcag ccggatttcg ccggcatcgg gtgcaagccc
     1801 gccggcaatc aggttgaaga gcgttgtctt gccggcgccg tttggaccaa taatcccgag
     1861 cgcctcgccc ggcgcgaccg aca
//
LOCUS       DASWGM010000377         2654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_580375,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000377 DASWGM010000000
VERSION     DASWGM010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580375"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VGA15_10945"
     CDS             <1..1344
                     /locus_tag="VGA15_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HEX9588245.1"
                     /translation="SGMHIQRQQMNDICRRAHSFDLVVAIGGPSVSACPDYYPSFDYL
                     HVGELGDATDDLIARLARDRSRPAQQVVLKTTDRLDMTEFPIPAYELADVKRYFLGSI
                     QYSSGCPYQCEFCDIPGLYGRNPRLKTPRQITAELDRLLECGVTGSVYFVDDNFIGNR
                     KAALDLLPHLIEWQKKTGYVMRLACEATLNIAKRPEILEKMREAYFVTIFCGIETPDP
                     DALKAMQKDHNMMVPILEGIATINRHGMEVVSGIIMGLDTDKPDTGNALLAFVDESRI
                     PLLTINLLQALPKTPLWDRLERENRLIDDETRDSNVDFRLPYDQVISSWRNCMEIAYQ
                     PEKLYARYQYQVDYTYANRLPLPRTAEQRTWANIKRGLTMLGKIFWKVGVLGDYRKVF
                     WKFALSRLRNGDIESLISVTLIAHHLIMFARAASGGQQNASNYSVRLREASVPAE"
     gene            1349..2149
                     /gene="eutC"
                     /locus_tag="VGA15_10950"
     CDS             1349..2149
                     /gene="eutC"
                     /locus_tag="VGA15_10950"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEX9588246.1"
                     /translation="MKTPAPASRSLTDLRDLTPARVGLGRAGASMPTKALLEFTLDHA
                     RARDAVHAAFDVFSLMSGLGDLGLAASDVSSRARNRRDYLRRPDLGRMLDPDSRQLLA
                     GRNGSPCQLAIVIGDGLSPSAVNAHAIELLRSLVPRLAADGIEIGAAVVASGARVALG
                     DEIGGLLGARMIVMLIGERPGLSAPDSLGAYLTFAPRPGLTDAERNCVSNIHGSGLGY
                     DEAASRIGWLIREGLAREITGVALKDESGDQTSRRITTNSTMCHKLET"
     gene            2353..>2654
                     /locus_tag="VGA15_10955"
     CDS             2353..>2654
                     /locus_tag="VGA15_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HEX9588247.1"
                     /translation="MLEKNSDSEVHAGKIEQRPTASIAFGLERLGLIAVQAPILSCII
                     LVALIVGAIFGIQRIKIDDSLSQLFRSNSKEYRQYEAETKRFPATEFDVLVVVEGKT"
BASE COUNT          518 a          803 c          803 g          530 t
ORIGIN      
        1 agcggcatgc atatccagcg tcagcagatg aacgatatct gccgccgcgc gcattcattc
       61 gatctggtgg tcgccatcgg cggaccctcg gtcagcgcct gcccggatta ctatccctcg
      121 ttcgactatc tccatgtcgg cgagctcggc gacgcgaccg acgacctgat cgcgcggctg
      181 gcgcgcgacc ggtcccgtcc cgcccaacag gtggtcctga agaccaccga ccgcctcgat
      241 atgaccgagt ttccgatccc ggcctatgag ctcgccgacg tcaaacgcta ttttctcggc
      301 agcatccagt attcgagcgg ctgcccctat cagtgcgaat tctgcgatat cccagggctg
      361 tacggccgta atccacgtct caagacgccg cggcagatca ccgcggaact cgaccggctg
      421 ctggaatgcg gggtgacggg ttcggtatat ttcgtcgacg acaatttcat cggcaaccga
      481 aaggcggcgc tcgatctgct gccgcatctg atcgagtggc agaagaaaac cggttacgtc
      541 atgcggctgg cctgcgaggc gacactcaac atcgccaagc ggccggaaat tctcgagaaa
      601 atgcgtgagg cctacttcgt cacgattttc tgcggcatcg agaccccgga cccggatgcg
      661 ctgaaggcga tgcaaaaaga ccacaacatg atggtcccca ttctggaggg gatcgcgacc
      721 atcaaccgtc acgggatgga agtggtctcc ggcatcatca tggggctcga tacggacaag
      781 ccggacaccg gcaacgcgct gcttgctttt gtcgatgaat ccaggattcc gttgctgacc
      841 atcaatctcc tgcaggcgct gccgaaaacg ccgctgtggg atcgcctcga gcgggaaaac
      901 cgcctgatcg acgacgagac ccgcgattcg aatgtcgatt tccggctgcc ctatgatcag
      961 gtcatcagtt cgtggcgcaa ctgcatggag atcgcctacc agccggaaaa gctgtacgcg
     1021 cgttaccagt accaggtgga ttacacctat gccaaccggc tgccgctgcc taggaccgcg
     1081 gagcagcgga cctgggccaa tatcaagcgt ggcctgacca tgctcggcaa gattttctgg
     1141 aaggtcggtg tgctcggcga ctaccgaaaa gtgttctgga aatttgcgct gtcgcgcctc
     1201 cgaaacggcg atatcgagag tttgatttcg gtgacgttga tcgcacatca cctgattatg
     1261 ttcgcgcgcg cggcttcggg cggtcagcag aatgcatcga attattcggt gcggctgcgc
     1321 gaggcgtccg tcccagccga gtaacgagat gaaaaccccg gcaccggcgt cccgttcgct
     1381 gacggatctg cgggatttga cgccggcgcg cgtcgggctt ggccgggccg gcgcaagcat
     1441 gccgaccaag gcactgctcg aatttacgtt ggaccatgcg cgcgcccgcg acgcggttca
     1501 tgccgccttc gacgttttca gtctcatgtc aggcctcggc gatcttggtc ttgcggcatc
     1561 ggacgtttcc agtcgtgcgc gcaaccggcg agactatctt aggcgtccgg acctcggccg
     1621 aatgctcgat ccggattccc ggcagctgct ggccggccgc aacggcagcc catgccagct
     1681 tgcgatcgtc attggcgatg gtctgtcacc atccgcggtc aatgctcacg cgatcgagct
     1741 cctccgcagc ctcgttccgc gattggccgc cgatgggatc gaaatcggcg ccgccgtggt
     1801 ggcgtcgggc gcgcgggtcg ctttgggcga tgagatcggc ggccttcttg gggcgcgcat
     1861 gattgtcatg ttgatcggtg agcggccggg cctgtccgca ccggacagtc tgggagccta
     1921 tctgaccttc gcgcccaggc ccggcctcac cgatgccgag cgaaattgcg tctccaacat
     1981 ccatggctcc gggcttggat acgatgaggc cgcgtcgcgg attggatggc tgatccgcga
     2041 aggcttggcc cgggaaatta ccggcgtcgc gctaaaggat gaaagtggcg accaaacgtc
     2101 ccggcgcatc acgacgaatt caaccatgtg tcacaaattg gagacttgaa gccattatcg
     2161 cgcatacgcg acgattttgc gcttgcgtgc gtgcttgcga gacgccgcat agacctgcta
     2221 gacctgtcgc ggtaaattta ccgcgtgttt tcaagggcta gcgccgatca tacgtggcca
     2281 atcgagcctc attcgcgccc ccgaatttaa gctatatgta taaatagagc cgacgtgaga
     2341 actggatagg gcatgctcga aaagaacagc gacagtgagg tccatgccgg gaagatcgag
     2401 cagcggccga cggccagcat cgccttcggt ctggagcgac tggggctgat cgccgttcag
     2461 gcgccgatcc tgtcctgcat cattctcgtt gcgctgattg tcggcgcgat cttcggtatc
     2521 cagcgcatca agatcgacga ttccctcagt cagttgttcc ggtccaattc caaggaatac
     2581 cggcaatatg aagcggaaac caagcgcttc ccggcgacgg aattcgacgt gctggtcgtg
     2641 gtcgaaggca agac
//
LOCUS       DASWGM010000378         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1704743,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000378 DASWGM010000000
VERSION     DASWGM010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704743"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGA15_10960"
     CDS             complement(<1..359)
                     /locus_tag="VGA15_10960"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX9588248.1"
                     /translation="MSQSPPAPPSLEVLRKEIDAIDEQVHRLLMARGDIIDRLISVKQ
                     TQEVGSAFRPAREADMMRRLVERHRGILPLDTVESIWRVIISTFTYVQAPFSVHADIS
                     LGESAMRDSARFHFGFTV"
     regulatory      539..618
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            710..>1645
                     /locus_tag="VGA15_10965"
     CDS             710..>1645
                     /locus_tag="VGA15_10965"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX9588249.1"
                     /translation="MVGVQSIPVPAIHSEERAHEADHPSSLLAVFGADQPLRLDCGID
                     LLPFQIAYQTYGELNADRSNAILIFHALTGDQHVANVHPVTGKPGWWETMVGPGRPLD
                     TDKYFIICSNVIGGCMGSTGPASTNPATGKLWGLDFPIITIPDMVRAQTMLIDRLGID
                     TLFCVVGGSMGGMQALQWTTAYPDRVFSALAVACSTRHSAQNIAFHELGRQAVMADPD
                     WHHGRYFEQGTHPHRGLGVARMAAHITYLSDAALHRKFGRRMQDRDLPTFSFDADFQV
                     ESYLRHQGSSFVERFDANSYLYLTRAMDYFDIAADH"
BASE COUNT          309 a          490 c          517 g          329 t
ORIGIN      
        1 acggtgaagc caaaatgaaa ccgcgccgaa tcccgcatcg cggactcgcc gagcgaaata
       61 tcggcgtgca ccgaaaacgg cgcctggaca taggtgaaag tcgagatgat gacgcgccag
      121 atgctctcga cggtgtcgag cggcaggatg ccgcgatggc gctcgaccag ccggcgcatc
      181 atgtcggcct cgcgcgcggg acgaaacgcc gagccgacct cctgggtctg tttcaccgaa
      241 atcagccggt cgatgatgtc gccacgcgcc atcagcaggc ggtggacctg ctcgtcgatg
      301 gcgtcgatct ctttgcgcag cacctcgagc gagggtggcg cggggggcga ttgagacatc
      361 ttccaaacct gttggatccg cggggcaagg ggcattgagg ggcattgagg ggcattgatt
      421 aggtaagacg gacgctgaaa gcaaagaaaa cgttaaggct tgtggctggg tcaccggcga
      481 tcagggcttc gccaaccaga ggcttgtctt gacgaaaagc cggccaatgg ctattattta
      541 gtcattccgt ggtcatttga gccggccggc ttgcagccac gttaaacaac tcgctaaaca
      601 ggccggggac agtagtgatc ccggccgaac ctatgttcag gccggttttt tttatggcct
      661 gatttcctaa ggtggtctgt gagacctcgt ttaagtggtc ggtgtcaaaa tggtaggcgt
      721 gcagtcgata ccagttccgg cgatccattc cgaggagcgc gcccacgaag cggaccatcc
      781 gagttcgctg ctggctgtgt tcggcgccga tcagccgttg cggctcgatt gcggcatcga
      841 tctcttgccg ttccagatcg cctatcagac ctatggcgag ctcaatgccg accgatccaa
      901 cgcgattttg atcttccacg cgctgaccgg cgatcagcat gtcgccaacg ttcacccggt
      961 gaccggcaag ccgggttggt gggaaaccat ggtcggcccc ggccggccgc tcgataccga
     1021 caaatatttc atcatttgct cgaacgtgat cggcggctgc atgggctcga ccggaccggc
     1081 atcgaccaac cccgccacag gcaagttgtg ggggctggat tttccgatca tcaccattcc
     1141 cgacatggtg cgcgcgcaga ccatgctgat cgaccgcctg ggcatcgata cgctgttctg
     1201 cgtggtcggc ggctcgatgg gcggcatgca ggcgttgcag tggaccaccg cctatcctga
     1261 ccgggtgttc tcggcgctgg cggtcgcctg cagcacgcgt cactcggcgc agaacatcgc
     1321 gtttcacgaa ctcggccgcc aggcggtgat ggccgacccg gattggcacc acgggcgcta
     1381 tttcgagcag ggcacccatc cgcatcgcgg cctcggcgtc gcgcggatgg cggcgcatat
     1441 cacctatctc tctgacgcag cgctgcatcg caagttcggg cggcggatgc aggaccgcga
     1501 tttgccgacg ttctcgttcg acgcggattt tcaggtcgag agctatctgc gccaccaggg
     1561 ttcgtccttt gtcgagcggt tcgacgccaa cagctatctc tacctgacgc gggcgatgga
     1621 ctattttgac attgccgccg atcac
//
LOCUS       DASWGM010000379         8413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1978518,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000379 DASWGM010000000
VERSION     DASWGM010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8413
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978518"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VGA15_10970"
     CDS             <1..588
                     /locus_tag="VGA15_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX9588250.1"
                     /translation="ELQLQQPHVVRLETRPPNIEGEGQVSMRELVRNCLRMRPERIIV
                     GEVRGPEAFDLLQAMNTGHDGSMGTLHANNPREALSRCESMITMGGFSLPSRTIREMI
                     CASIDVIIQAARLRDGSRRITHITEVMGMEGDTIITQDLFLYDMVGEDANGHIIGRHR
                     STGIGRPRFWDRARYYGEEKRLAAALDAAEVTSEV"
     gene            600..1574
                     /locus_tag="VGA15_10975"
     CDS             600..1574
                     /locus_tag="VGA15_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9588251.1"
                     /translation="MNIQALALAFLAAAAVGGISWVFLYPMLSGERKAEHRRASVAKS
                     EPAARNVDKNQRSRREQVEGSLKDLEARRQKEKKIPLSSRLTQAGLTWTTQKFMVASG
                     VLAAVAFAIAMLAGGGLIAAVGLAFAAGFGLPRWALSYLKKRREKAFLKALPDAVDVI
                     VRGIKAGLPLFESIKVVAADAPEPLKGEFLAIIETQAIGMPLGDACARLFDRMPVPEA
                     NFFGIVIAIQQKSGGNLSEALGNLSKVLRDRKKMAEKIQAMSMEAKASAGIIGSLPPI
                     VMLLVYLSTPDYISLLWTHPAGQLMLVGCVVWMSIGIFVMKRMINFDF"
     gene            1584..2558
                     /locus_tag="VGA15_10980"
     CDS             1584..2558
                     /locus_tag="VGA15_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9588252.1"
                     /translation="MFSFLVEKIHDARFVSMVLAAIAVSATAYTLMMPLFAGEGLSKR
                     MKAVASERERIRQRERERLSKNEKVSLRQTPKQLVSKVVEDFNLGKWLAQEAAREKLT
                     MAGYRGHAPYVTFLFFRLVTPIVFFVGAVLYVFVISHMEKSTPVKIGMCIGAAYFGLQ
                     APMMFLKNAISKRQLSIKRAFPDALDLLLICIESGMSIEVAFRKVSTEIVTQSVALSE
                     EFTLTTAELSYLQDRKVAYENLAKRTGLEGVKSVCLALQQSERYGTPLGQSLRVMAQE
                     NRDMRMNEAEKKAAALPPKLTVPMILFFLPVLFVVILGPTFIKVAAMQ"
     gene            complement(2741..3550)
                     /locus_tag="VGA15_10985"
     CDS             complement(2741..3550)
                     /locus_tag="VGA15_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9588253.1"
                     /translation="MRQQSSVVRLLASAAVAAILAVGLGGCQTTMSDITGSLSPKAET
                     VPDADPRRSVDVYGERYRANPKDADAALLYGQALRATGQRSQAVAVLEQATIAHPGNK
                     ALLAGYGRALADNGNFQQGFDVLTRAHSPDNPDWRILSVQGTVLDQLGRHDEARRYYE
                     SALKIVPEEPSVLSNLGLSYVLSRDLPKAEEALRRAYGSTRADARVRQNLGLVVGLQG
                     RFAEAESIVKADLPAEEAAANVAYLKQMLSRKDSPRNGDGTVPVASLSRPN"
     gene            3740..4033
                     /locus_tag="VGA15_10990"
     CDS             3740..4033
                     /locus_tag="VGA15_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588254.1"
                     /translation="MLRNFFRRIVIAALLFAATAAVAAPVQAKRHVVHRWHGYGFLPG
                     YRSPERIEAEQARNRGPVYWYGGPGFYRGRWNGGGFGPCWTQTPIGNVWNCGR"
     gene            complement(4178..4537)
                     /locus_tag="VGA15_10995"
     CDS             complement(4178..4537)
                     /locus_tag="VGA15_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588255.1"
                     /translation="MARHRTSYRDILTRGLAVIALLCIYGLSVIGASALLLGASTTSA
                     LARGGGRGGGGGFRGGGGFRGGGFRGAGFGLGLGYPYYGYGGYYPYYGDAGVCYEQRR
                     RVLTPYGWRIRRVSVCE"
     gene            4810..6192
                     /locus_tag="VGA15_11000"
     CDS             4810..6192
                     /locus_tag="VGA15_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX9588256.1"
                     /translation="MHSVFETAPATTAIPITFATKATWEAICAALPAQARQFALANGF
                     AAKPGACLTLPASDGQIAHVLFGLEDETSKFPDPFRPGALPGLLPAGVYRFANAPHDT
                     RLATLAFALGAYRFGRYRKADAPDVKLVPPDGIDVAAVARMAEAAALARDLINTPSND
                     MGPEELASAAELLAQRFGATFGCIVGDDLIRQNFPLIHAVGMASSHAPRLIDLSWGDP
                     AHPKVTLVGKGVCFDTGGLDLKPSSGMLIMKKDMGGAANVLALALMVMDAKLKVRLRV
                     LIPAVENAVAGNAFRPLDIFKSRKGLNVEIGNTDAEGRLVLADALALACEEKPDLLID
                     LGTLTGAARVALGPDLPPFYTNDETLALDVARCAKQENDPLWRMPLWPPYDAWLDSKV
                     ANINNAPSGTFGGSITCALFLQRFVEDATSWLHVDIYGWTPSAKPARPEGGECQAARA
                     IFQLLGERYG"
     gene            6185..7030
                     /locus_tag="VGA15_11005"
     CDS             6185..7030
                     /locus_tag="VGA15_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX9588257.1"
                     /translation="MDDPRLTPARPDLAAKYLEGKVKAKRFVIGEEFEIVDALAPLRE
                     GPSSDAMLLTQALMGERVTIYDRNGEGFAWGQLNSDGYVGWLPDRTLAKPTGKPTHKI
                     TALRTFAFPGPSIKLPPVDTLVMGATIAVIREDGAFAVTRDGWHLPLPHVGRIDRHAD
                     DFVGVAEQFAGTPYLWGGKSSLGIDCSGLVQVSLNAAGTGCPRDSDMQQDSLGRALDA
                     SESKRLQRGDLIFWKGHVAIVRNADTIVHAHAHHMATTIENTSNAIARIKAAGSEILA
                     IKRLA"
     gene            complement(7258..>8413)
                     /locus_tag="VGA15_11010"
     CDS             complement(7258..>8413)
                     /locus_tag="VGA15_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9588258.1"
                     /translation="GGQRQRVMIAMALANEPDLLIADEPTTALDVTVQAQILTLLAEI
                     RARLGMSLLFITHDLGIVRRIADVVCVMNGGKIVEQGPVEQVFTAPKHPYTRALLAAE
                     PKPDPAPPRPNSSVVISADDLKVWFPVKRGLLRSTVGYIKAVDGVSLKVRKGETLGVV
                     GESGSGKTTLGLALLRLISSDGPIVFLGKNIQGLRFKAMRPFRRDMQIVFQDPFGALS
                     PRMSVGDIVAEGLSVHQRALSEHEREVRVIKALRDVGMDPATRFRYPHEFSGGQRQRI
                     SIARAVVLEPNFVVLDEPTSALDMLFQAQMVDLLRELQRKRDLTYMFISHDLRVVASL
                     ASHLIVMREGKVVEEGPASELFKNPKSDYTRALFAAAFRLEAAPGGVLEI"
BASE COUNT         1611 a         2629 c         2626 g         1547 t
ORIGIN      
        1 gaacttcagc tgcagcaacc tcatgtggtg cgcctggaaa cccgtccacc caacatcgag
       61 ggtgaaggtc aggtatcgat gcgcgaactg gtccgtaact gcctccgcat gcgtcctgaa
      121 cgcatcatcg tcggcgaagt ccgcggaccg gaagcgttcg acctgctgca ggccatgaac
      181 accggccatg acgggtcgat gggaacgctg cacgccaaca atccccgcga agccctgtcg
      241 cgttgcgaat cgatgatcac gatgggcgga ttttcgcttc cgtcacggac gatccgcgaa
      301 atgatctgcg cctcgatcga cgtcatcatc caggccgccc ggctgcgcga cggctcccgg
      361 cgcatcacgc acatcaccga ggtgatgggc atggaaggcg ataccatcat tacccaggac
      421 ctgttccttt acgacatggt cggcgaggac gccaacggcc acatcatcgg ccgacatcgc
      481 tcgaccggca tcggacggcc gcgcttctgg gatcgcgcaa gatattacgg cgaagagaag
      541 cggctcgctg ccgcgctcga tgccgctgaa gtcacctcag aggtttgaag gagcgggcca
      601 tgaacatcca agcgctcgca ctggcgtttc tcgcggccgc cgccgtcggc ggtatctcgt
      661 gggtttttct ttatcccatg ctgtcgggag agagaaaagc cgagcatcgc cgggcgtccg
      721 tagcgaagtc ggagccggct gcgcgcaatg tcgacaaaaa ccagcgctct cgccgcgaac
      781 aggtcgaggg gtcgctaaag gatctcgagg cgcgtcgcca gaaggaaaag aagatcccgc
      841 tcagcagccg tctcacccag gccggcctga cctggacaac tcagaaattc atggtcgctt
      901 ccggcgttct cgccgccgta gcctttgcga tcgcgatgct ggccggcggc ggattgatcg
      961 cggccgtcgg cctggccttc gctgcgggtt tcggtctgcc gcgctgggcc ttgagctatc
     1021 tcaagaagcg ccgggaaaag gcgtttctca aggcactgcc ggatgccgtc gacgtgatcg
     1081 ttcgcggcat caaggcgggc ctgccgctgt ttgaatccat caaggtcgtc gccgccgatg
     1141 cgccggagcc gctcaagggc gagttcctcg ccatcatcga aacccaggcg atcggcatgc
     1201 cgcttggcga tgcatgcgcc cgcctgttcg atcggatgcc ggtgccggag gcgaatttct
     1261 tcgggatcgt gatcgcgatc cagcaaaagt ccggcggcaa cctgtcggag gccttgggca
     1321 acctgtccaa ggtgctgcgc gatcgcaaga agatggccga gaagattcag gcgatgtcga
     1381 tggaagccaa ggcgtccgcg ggcattatcg gctcgttgcc gccgatcgtc atgctgctgg
     1441 tctacctttc gacccccgac tacatctcgc tgctgtggac ccatccggcc ggtcaattga
     1501 tgctggttgg ctgcgtggtc tggatgtcca tcggcatttt cgtaatgaag agaatgatca
     1561 acttcgactt ctgatggtgc agcatgttta gttttctggt cgagaagatc cacgacgcac
     1621 ggttcgtttc catggtgctc gccgctattg ccgtgagcgc taccgcgtac acgttgatga
     1681 tgccgctgtt cgccggcgaa ggactgtcca agcgcatgaa ggccgtcgcc agcgaacgcg
     1741 agcggatccg gcaacgggag cgcgaacgtc tctccaagaa cgaaaaggta tcgctgcggc
     1801 agaccccgaa gcagctcgtc tccaaggtgg tggaagactt caaccttggg aaatggctgg
     1861 cgcaggaggc cgcgcgcgaa aagctgacca tggccggcta tcgcggccac gcgccctacg
     1921 tcacgttcct gttcttccgg ctggttactc ctatcgtgtt tttcgtcggc gcggtgctgt
     1981 atgtcttcgt gatctcgcat atggagaagt cgacgccggt gaaaatcggc atgtgcatcg
     2041 gcgcggccta ttttgggctg caggcgccga tgatgtttct gaagaacgcg atctcaaagc
     2101 gccagctctc gatcaagcgc gcctttcccg atgcgctcga tctgctgctg atctgcatcg
     2161 agtccggcat gtcgatcgaa gtggccttcc gcaaggtctc aaccgagatc gtgacgcagt
     2221 cggttgcgct gtcggaggaa ttcacgctga ccacagccga gctctcctat ctgcaggacc
     2281 gcaaggtggc ctacgagaac ctggcaaaac gcaccggcct cgagggcgtg aaatcggtct
     2341 gtctggcctt gcagcaatcg gaacgttacg gcacgccgct cggccagagc ttgcgcgtca
     2401 tggcgcagga aaatcgcgac atgcgcatga acgaggccga gaagaaggcg gccgcgctgc
     2461 cgccgaaact gacggtgccg atgattctgt tcttcctgcc ggtgttgttt gtcgtcatcc
     2521 tgggtccgac cttcatcaag gtcgcggcga tgcagtgagc cgggccgatc cgataaattg
     2581 gtagcctgcc ccaaaacatt cggttgcgaa gtttcaccat ctgatgccgt cgtcgcccgg
     2641 cttgatgtat ggaccgggcg atccggtaaa cgctgacgcc ggcgattact ggatcacacg
     2701 ctctttcgcg ggtgatgatg cctccgatcg gagcaatcga tcagttggga cggctgagcg
     2761 acgccaccgg cacggtgccg tcgccgttgc gcgggctatc cttgcggctc agcatctgct
     2821 tcaggtaggc gacgttcgca gccgcctcct ccgccggcag atcggccttc acgatgcttt
     2881 cagcttcggc aaagcggccc tgcaggccaa ccaccagacc cagattctgc cgcacccggg
     2941 catcggcgcg ggtgctgccg taagcgcggc gaagcgcctc ctcggccttc ggcagatccc
     3001 tggaaagaac ataggagagg ccgagattgg acagcaccga gggctcctcg ggcacgatct
     3061 tcaacgcgct ctcgtagtag cggcgcgctt cgtcatgccg gccgagctga tcgagcacgg
     3121 ttccctgcac cgaaagaatt cgccaatcgg gattgtcggg cgaatgggcg cgggtcagaa
     3181 cgtcgaagcc ctgctggaaa ttgccgttat cggcaagcgc gcggccgtag ccggcgagca
     3241 gcgccttgtt gccgggatga gcgatggtgg cctgttcgag cacggcgacg gcctggctgc
     3301 gctgccccgt ggcgcgcagc gcctggccat agagcagggc cgcgtcggcg tccttgggat
     3361 tggcgcgata gcgctcgccg taaacatcca ccgaacgccg cggatcggcg tcggggacgg
     3421 tctcagcctt gggtgacaac gatccggtga tgtcggacat ggtcgtctgg cagccgccga
     3481 ggcccacggc caaaatcgcc gccacggccg cagatgcaag aagccggaca acactggact
     3541 gctggcgcat agagctttaa ctcacggtat cagacgaatt gttggagccg aatgcgctca
     3601 gcaataaacc gttaacccta acacccggtt aatccgcagc ttccgcgaaa atcgttcgtc
     3661 gtcagtggat ttacggggcg attgacccac ccgccgtacg ctacgcggtg attgaaccag
     3721 tggaacagga caacccgcaa tgttgcgaaa tttctttcgt cggatcgtga ttgcagcttt
     3781 gctgttcgca gccacagccg ctgttgctgc gcccgtgcag gcaaagcgcc acgtggtgca
     3841 tcgctggcac gggtatggtt tcttgcccgg ctaccgttca cccgaacgga tcgaagcgga
     3901 gcaggcgcga aatcgcgggc cggtctattg gtatggcggc ccgggttttt atcgagggcg
     3961 ctggaacggc ggcgggttcg gcccgtgctg gacccagacc ccgatcggca atgtgtggaa
     4021 ctgcggtcga tagcgggatc gcggccccgt gcgccgctcg gcccaccaaa aagacatggc
     4081 ctcagttgaa gacactgagg ccgatcggtc ccgggatttt cggatagatc gacaattcgg
     4141 tccgtgaccg ttcaggcgca gcgccgcaga gccgccatca ctcgcagacc gagacgcggc
     4201 gtatgcgcca accgtagggc gtcaagacgc gtcgccgctg ctcatagcaa acgcccgcgt
     4261 cgccataata gggatagtat ccgccgtagc cgtaataggg atagcccaga cccagaccaa
     4321 atccggcgcc gcgaaaacca ccaccacgga agccgccgcc gccgcggaaa ccaccgccgc
     4381 cgccacgacc tccaccacga gcaagggcag atgtggtaga ggcgcccaac aagagagccg
     4441 acgcgccaat gacactgagg ccatagatgc aaagcaaagc gatgactgcc aagccgcgcg
     4501 tcaggatatc gcgatagctc gttctgtggc gggccattgg ttctctcact cggtgtcctg
     4561 ttcgcgcaga cagcatttgt tatcaaaagc gtaggtttga gtcacgcttt cggtcaaatc
     4621 acctcgacgt catcgtcgtg aagctcaagg gggtgctcga gtgccggccc tcaaacacgc
     4681 ctcgatattc gaagggttca gactggcttg acattgtaag tcattgtaga agcaccaagg
     4741 ctcgcaacgt ctggctgatt gccactccgg cctctgcccg ccccgtttca attgaagacc
     4801 gagagaagca tgcattccgt attcgagacc gcgcctgcca cgacagccat tcccattacg
     4861 tttgcgacca aggcgacatg ggaggcgatt tgcgctgctc ttcccgcgca ggcccggcaa
     4921 ttcgcgctgg ccaatggctt tgcggccaag ccgggcgcgt gcctcacgct gcccgcttcg
     4981 gatggacaga tcgcgcacgt gctgttcggc cttgaggacg agaccagcaa attccccgac
     5041 ccgttcaggc ccggcgcgtt gccgggcctg ctgccggcgg gcgtctatcg cttcgccaat
     5101 gcgccgcacg acacccgtct cgccacgctg gcgtttgcgc tcggcgctta tcgcttcggc
     5161 cgctatcgca aggcggatgc gcccgatgtc aaactggtgc cgcccgatgg cattgacgtt
     5221 gcggccgttg cgcgaatggc ggaagcggcc gcgcttgccc gcgacctcat caatacgccg
     5281 tcgaacgaca tgggaccgga agagctggcg tcggcggccg agctgctggc gcaacgcttc
     5341 ggcgccacat tcggctgcat cgtcggcgac gatctgatcc gacaaaattt tcccttgatt
     5401 catgcggtcg ggatggcgtc gtcgcacgcg ccgcggctga tcgatttgag ctggggcgat
     5461 cccgcccacc ccaaggtgac gctggtcggc aagggcgtgt gcttcgatac cggcgggctg
     5521 gacctgaaac catccagcgg catgttgatc atgaagaagg atatgggcgg ggccgccaat
     5581 gtactggcgc tggcgctgat ggtgatggat gcaaagctca aggtgcgctt gcgggttttg
     5641 attcccgcgg tcgagaacgc ggtcgccggc aatgccttcc gtccgctcga tatcttcaaa
     5701 tcgcgcaagg gccttaacgt ggagatcggc aacaccgacg ccgagggccg gctggtgctg
     5761 gccgacgcgc tggcgctcgc ctgcgaggaa aagcccgatt tgctgatcga tttgggcacc
     5821 ctgaccggcg cggcgcgggt ggcgctgggt ccggatctac cgccctttta caccaatgat
     5881 gagacactgg cgctggacgt cgcacgttgc gccaaacagg agaacgatcc gttgtggcga
     5941 atgccgctgt ggcctcctta cgatgcctgg ctcgattcga aggtcgccaa tatcaacaac
     6001 gcgccgtcgg gcacgttcgg gggctcgatc acctgcgcct tgttcctgca gcgtttcgtc
     6061 gaggatgcaa cgagctggct gcatgtcgat atctacggct ggacgccatc ggcaaagccg
     6121 gcgcggcccg agggcggcga atgccaggcc gcccgcgcga tcttccaact gctgggcgaa
     6181 cgctatggat gatccgcgcc tgaccccggc acggcccgac ctcgccgcga aatatctcga
     6241 aggcaaggtc aaggccaagc gttttgtcat tggcgaggaa ttcgaaattg tcgatgcgct
     6301 cgcgccgctg cgcgaaggtc cctcttccga tgccatgctg ttgacgcagg cgctgatggg
     6361 cgaacgcgtc acgatttatg accgcaacgg cgaaggattt gcctggggcc agttgaacag
     6421 cgatggctat gtcggctggc ttcccgaccg tacgctggca aaacccaccg ggaagcccac
     6481 acacaagatc acggcgctca gaacgttcgc gtttccgggt ccctcgatca aactgccgcc
     6541 ggtcgatacg ctggtcatgg gagcaaccat cgccgtgatc cgcgaagacg gcgctttcgc
     6601 cgtgacgcgc gacggttggc acctgccgct gccgcatgtc ggccgcatcg atcgccatgc
     6661 ggacgatttt gtcggcgtcg ccgaacaatt cgctggcacg ccgtatctgt ggggtggcaa
     6721 gagcagtctt gggatcgact gttccgggct ggtgcaggtg tcgttgaacg ccgcgggtac
     6781 cggctgcccg cgcgacagcg acatgcaaca ggacagcctc ggccgggcat tggacgcgtc
     6841 ggaatcgaag agattgcagc gcggcgacct gattttctgg aaaggccatg tcgcgatcgt
     6901 gcggaacgcc gacaccatcg tgcacgccca cgcgcatcac atggccacga caatcgaaaa
     6961 caccagcaac gccatcgcgc gcataaaagc cgcgggtagc gaaatcctgg cgatcaagcg
     7021 gctggcttga tctaagaaga tctaagaata tagaatcgaa gcgcgggagt tcggccacct
     7081 ctcccatggg gagaggtcgg atcgctcttg cgatccgggt gaggggttac gacctaacga
     7141 gagacctgaa cccctcaccc caaccctctc ccacaagggg agagggagcg cgctgccgtt
     7201 gcttcaacct catcatgata tctcaaccca agtggaacaa acccagctga acgctgttca
     7261 aatttcgagg acaccacccg gtgcggcttc cagccggaac gcggcggcaa acagcgcgcg
     7321 cgtgtaatcg gatttaggat tcttgaacag ctccgacgcc ggcccctcct ccactacctt
     7381 gccctcacgc atcacgatca gatggctggc gagcgaagcg accacgcgca aatcatgcga
     7441 gatgaacatg taggtcaggt cgcgcttgcg ctgcagttcg cgcaacagat cgaccatctg
     7501 cgcctggaac agcatgtcga gcgcgctggt cggctcatcc agcacgacga aattcggctc
     7561 cagcaccacc gcgcgggcaa tgctgatgcg ctggcgctgg ccgccggaga attcatgcgg
     7621 atagcgaaac cgcgtcgccg gatccatgcc gacatcgcgg agcgccttga tcacgcgcac
     7681 ttcgcgctca tgttccgaaa gcgcgcgctg atgcacgctg aggccttcgg ccacgatgtc
     7741 gccgaccgac atgcgcgggc tcagcgcgcc gaacggatcc tggaacacga tctgcatgtc
     7801 gcggcggaac ggccgcatcg ccttgaagcg caggccctgg atgtttttgc ctaaaaacac
     7861 gatcggcccg tcggacgaga tcagccgcag cagcgccagg ccgagcgtgg tcttgccgga
     7921 gccggattcc ccgaccaccc ccagcgtctc gcccttgcgc accttgagac tgacgccatc
     7981 gaccgccttg atatatccga ccgtactgcg cagcaggccg cgcttgaccg gaaaccagac
     8041 cttcaaatca tccgccgata tcacgaccga cgaattgggc cgcggtggcg ccgggtcggg
     8101 ttttggttcg gccgccagca aggcccgcgt ataagggtgc tttggcgcgg tgaagacctg
     8161 ctccaccggg ccctgctcga cgatcttgcc gccgttcatg acgcagacca cgtccgcaat
     8221 gcggcgaacg atgccgaggt catgggtgat gaacaaaaga ctcatgccga gtcgggcgcg
     8281 gatctccgcc agcagcgtga ggatctgcgc ctgcacggtg acgtcgagcg ccgtggtcgg
     8341 ctcgtccgcg atcagcagat ccggctcgtt ggcaagcgcc atcgcgatca tgacacgctg
     8401 gcgctggccg ccg
//
LOCUS       DASWGM010000380         9600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_42280,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000380 DASWGM010000000
VERSION     DASWGM010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42280"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..87
                     /locus_tag="VGA15_11015"
     CDS             <1..87
                     /locus_tag="VGA15_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX9588259.1"
                     /translation="LSDPHRRNGCAEHVIIATAEAYSKFKIG"
     gene            complement(74..706)
                     /locus_tag="VGA15_11020"
     CDS             complement(74..706)
                     /locus_tag="VGA15_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9588260.1"
                     /translation="MPRRPYTSSVRTAAAAENRERVIKAAARSLREDASIASFSLDAV
                     AKAAGVTRLTVYHQFGSRRGLFEAVFDEIARQGGLAEIPDAMAMSDPRAALDRLVEIF
                     CAFWNRDPAVGRLHEAMATDPEFAQALIGRNERRRETVNVLVGRIAGKTAPPRARRGA
                     VDMIFALTSFAMFAMLRRDRSVDDVCALLKTACRNALEPLLPDRRRSSRS"
     gene            785..1186
                     /locus_tag="VGA15_11025"
     CDS             785..1186
                     /locus_tag="VGA15_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003577166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588261.1"
                     /translation="MQIVLIIALSLHILSSVFWAGTSFTLARTGGVGGVQLFRPQMGA
                     AVIAVLTGGYLGHLVHAGAFGTAEQILSFGALAALAAAGVQGAIGGRAIRSLRNGMDE
                     AGARSHIATAQRIAAALLAVTAVCMGAARYA"
     gene            1967..3883
                     /locus_tag="VGA15_11030"
     CDS             1967..3883
                     /locus_tag="VGA15_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9588262.1"
                     /translation="MTIELVAATAPMTIEPVAARHTRRLMAVMQADVVGYSRMMSRSE
                     DETHARFAGHARELIDPTIKKYGGRLVRSMGDGLLVEFVSAVDAVRCALDIQRGLAAR
                     QPNKPDRMQLRIGINTGDVLVDQHDIYGNNVNIAARLEALALPGTVCVSQGIYDQMRA
                     QPDFFFAYRGARRVKNIPYPVHVYEVANERIRVTLLAWLVAQGGRLAAGASFGAMAIA
                     SIAAVLIFREPHQVVARTNTIVVLPFKNVDGNTADDYLADAITDELTTELSRLRRAWV
                     IASGTAFVYKGKPTDPRQVGRELQVRYALEGSVKRAGLTVQVNAQLIDTESGTNLWAN
                     RFAHETSSLLDLQDNLLERIATSLNDEMAKDAVRHEVGTLAADANPLDERMRAMAANT
                     GFPTPQKSLETRHHAETGLKADPDNARLLGLLANVLASDVLNAWNGAGKPEVDRAEVA
                     AKKAISLDHNTAIAHYALGFVHRLRGNHEAALDAFNEAIKIDSNLAKAYAQAANSMVF
                     IGNARGPIPLAEKAAQLSPQDPSIGVFLWVKGRAYFTLGDYPKAIEALEESVRARPNL
                     WFTQAWLVAAYALSNKDAEARKALGAFKKTPFSTRFDLDWITQYYKEEQYQNPTLQAA
                     SAEVLNGLRKAGLK"
     gene            complement(4080..4487)
                     /locus_tag="VGA15_11035"
                     /pseudo
     CDS             complement(4080..4487)
                     /locus_tag="VGA15_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2778 domain-containing protein"
     gene            5303..5896
                     /locus_tag="VGA15_11040"
     CDS             5303..5896
                     /locus_tag="VGA15_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX9588263.1"
                     /translation="MSSISCARTSHVDIRANRFLLNARLVSAAGVRNVIQLFAVALVA
                     ASLAACAQSSVVTRKSELLTASRQASLEQNRTTSFVTNRRVAVTRKHTPFASHKNAAE
                     TQVASHGLASFYTEGTQTANGEKFDTHELTAAHRTLPFGTRLRVTNVATGRSVTVRVN
                     DRGPFIPGRVVDVSYSAAQTLGMVGGGIAKVKLDVVQ"
     gene            complement(6055..6990)
                     /locus_tag="VGA15_11045"
     CDS             complement(6055..6990)
                     /locus_tag="VGA15_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9588264.1"
                     /translation="MSASDHTSSAVPSGNWLANQPYLLLSITALCWAGNAIVGRLAAG
                     HIPPVTLSFLRWSLALLVILPFAWKHLARDRGAIRSHLGTMIFLSVIGISAFSTLQYW
                     SLEHTTALNTLLLQSAGPLIVAVWSLVLFGVRLTLAQTGGVALSLTGVLVILLHGDLT
                     ALTSIAFNKGDIIFTVALAIFGLYSVLSLKRPAIHGLSFVAFTFGCGALCLVPLWIWE
                     LFARPVMQLNTANLLSLFYVAVFPSALAYLCFNRGVQLIGANRAAPFFHVVPVFGSAM
                     AIVFLGERPQLFHIIGFALVLTGVFVASRKPANVH"
     gene            7263..8438
                     /locus_tag="VGA15_11050"
                     /pseudo
     CDS             7263..8438
                     /locus_tag="VGA15_11050"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
     gene            8506..9258
                     /locus_tag="VGA15_11055"
     CDS             8506..9258
                     /locus_tag="VGA15_11055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX9588265.1"
                     /translation="MSCARRFNYDFQLGKLGHEVDRAEWLTTPQTVNAYYSASRNEIA
                     FPAGILQPPFFDAEADDAANYGAIGSVIGHEISHAFDDHGSRFDGDGNLRNWWTEDDR
                     TRFEGKTRSLVAQYDAFSPLPGHHVNGALTLGENVADNAGLAIAERAYRLSLGGCPAP
                     DIDGYTGEQRLFISFAQIWRDKVREAARIERLKVDPHSPGQFRADGTLRNQSAFADAF
                     AVKPGDAMYLPPEQRISLWQTDSEVVCKEKAR"
BASE COUNT         1996 a         2894 c         2941 g         1769 t
ORIGIN      
        1 ctgtccgatc cgcatcgccg caacggctgc gccgagcacg tgatcatcgc gaccgccgag
       61 gcctacagca aattcaagat cggctagaac ggcgccggtc gggcaacagc ggttcaagcg
      121 cgttgcggca ggccgttttc agcagcgcgc agacgtcatc gactgagcga tcccggcgaa
      181 gcatggcgaa catggcgaag ctggtgagcg cgaagatcat gtcgacggcg ccgcgccgcg
      241 cccggggcgg cgccgtcttc ccggcgatcc gtccgaccag gacattgacc gtttctcgtc
      301 tgcgttcatt gcgcccgatc agcgcctgcg caaattcggg atcggttgcc atcgcctcat
      361 gcagccggcc cacggccggg tcccgattcc aaaaggcgca gaagatctcg accagccggt
      421 cgagcgccgc gcgcggatcc gacatcgcca tcgcgtcagg gatctccgca aggccgcctt
      481 gtcgtgcgat ctcatcgaac acggcttcga acaatcctcg ccgtgatccg aactgatgat
      541 agacggtcag ccgtgtcacg cctgccgcct tcgccacggc atccagcgag aagctggcga
      601 tgcttgcatc ctcgcgcagc gatcgggccg cggctttgat gacgcgttcc cggttctctg
      661 cggcggcggc cgtccgaacg gaactcgtgt aggggcgtct cggcatcccg gcttcccgcc
      721 atcctttcgg ctaattgact atacatattg tatatctaat tacaacccat ccgggagcca
      781 tgccatgcag atcgtactca tcatagcctt gtcacttcat attctatcct ccgtgttctg
      841 ggcgggcacg agcttcacgt tggcgcgaac cggtggcgtc ggcggcgtgc agctttttcg
      901 tccgcagatg ggtgcggcgg tgatcgccgt cctgaccggc ggctatctcg ggcatctggt
      961 ccatgccggt gcctttggca cggccgaaca gatccttagc ttcggcgcgc tcgccgcgtt
     1021 ggccgctgcg ggcgttcagg gcgcgatcgg cggccgcgcg atcagaagct tgcgcaatgg
     1081 gatggacgag gccggcgcgc ggtcgcatat cgccacggcc cagcgtatcg ccgccgcgtt
     1141 gctcgcggtg acggctgtgt gcatgggagc cgcgcgctac gcgtgaaaga cgatttcggc
     1201 gaatggcggc atgctagtgc gtgaacccat aaatccagag gcgtgggcga acgtccgctt
     1261 tggtgtattc cggacgcaac tctgacatcg cgcaaggtcc gaaaagtgcc aatagtgttg
     1321 caaaaatccc aaaatgcggt gcggtcaatt tcccgcaaat agaccaatcg agccacaatc
     1381 gccgacagat gtagcctcca tgccattacc gaagtcgccc gtgggttcgt cgcgagataa
     1441 tgtagtccct caaatgatta ttcgatcgcc gcgcctatag cccggaaaat ttgtggtcga
     1501 tgttgcaaaa agacttttgc aacagtatct gccaatagcg ctcattcggg caagccgacc
     1561 aaacgatgct cggaccgcgc ttaacgttcc ctgcaagccg ctcgttgctc ttacttagcg
     1621 agtctcgcca tcactaacga gatgctcccg aaagaaccta acaacgctcg cgtcgaagtc
     1681 acggtgaaag gccgtcctgt cgaaggcccg gcgggtcggt gcaaacgcgc ggcatgttcg
     1741 ccgccagctg gggcgtgcac ggcggcaaaa aaagcatagc gaccggccag cacgacgtga
     1801 acgtcaggct ggccgggcag gctactggca acgcgcgagg acctcttcgg ggattgattg
     1861 aatcccaaca cacggattta acccagcaaa cgacagcgtt cgtaggactt gttttttgcg
     1921 attccggcgg cttcgagtat agtgacaatc aaaggaggct gcgccgatga caatcgagct
     1981 ggttgccgcg accgcaccga tgacaatcga gccggttgcc gcccggcaca cgcggcgatt
     2041 gatggcagtg atgcaagccg atgtcgtcgg ctacagccgt atgatgagcc gtagtgagga
     2101 cgagacgcat gcgcgctttg cgggccatgc gcgtgaattg attgacccga caattaaaaa
     2161 atatggcggg cgtctcgtgc gcagcatggg cgacggattg ctggtcgagt ttgtgagcgc
     2221 ggtcgatgcg gtacgttgcg ccctggatat ccagcgcgga cttgctgcgc gtcagcctaa
     2281 taagccggat cgaatgcagc tgcggatcgg aatcaacacg ggcgacgtcc tcgtcgatca
     2341 gcacgacatt tacggaaata acgtcaatat cgcggctcgg cttgaggccc tggctttgcc
     2401 gggaacggta tgcgtcagcc agggcatcta cgatcagatg cgcgctcaac ccgacttttt
     2461 cttcgcgtac cgcggggccc gccgtgtaaa aaacatcccg tacccggtcc acgtgtacga
     2521 ggtcgccaac gaacggattc gggtgaccct ccttgcgtgg cttgtcgcgc aggggggcag
     2581 attggcggca ggggccagct ttggggcgat ggcaatcgcc tcgatcgccg cagtgctgat
     2641 atttcgcgag ccgcatcagg ttgttgcgcg gaccaatacg atcgtcgtgt tgccgtttaa
     2701 gaacgtcgac gggaacacgg ccgatgatta tttggccgac gcaatcactg acgagctgac
     2761 gaccgagctt tcccggcttc ggcgcgcttg ggtgatcgcc tccggaacgg cgttcgtgta
     2821 caaaggcaag ccgaccgacc ctcgccaggt cggccgggag ctccaagtcc gctacgccct
     2881 tgaagggagc gtaaagcgcg caggtctgac ggtccaggtg aatgcacagc tgatcgacac
     2941 cgaaagcggt accaaccttt gggccaatcg cttcgcgcac gaaaccagca gcctgctcga
     3001 cctgcaggac aacctattgg agcgaatcgc tacttcgctg aatgacgaga tggcaaaaga
     3061 tgccgtacga cacgaggtcg gcactctggc agcggatgcc aacccgctag atgaaaggat
     3121 gagagcaatg gctgcaaaca ctggtttccc aacgccgcaa aagtcgcttg aaacgcgcca
     3181 ccatgcggag acaggcctca aagccgatcc ggacaatgcg cggcttttgg gtctgctcgc
     3241 caacgttctg gcttcggatg tactcaatgc ctggaatggc gcaggcaagc ctgaggtcga
     3301 tcgcgcagag gtggccgcaa aaaaagcaat aagcctcgac cacaatacgg cgatcgcgca
     3361 ctacgctctc ggattcgtcc atcggttacg tggcaatcac gaagcggcgc tggacgcctt
     3421 caatgaggcg atcaagatcg actccaacct tgccaaagcc tatgctcagg ccgcaaattc
     3481 gatggtcttc atcggcaatg cacgtggccc gattccactg gccgaaaagg cagctcaact
     3541 tagcccgcag gacccgtcaa tcggggtttt cctctgggtc aaaggacgcg cttatttcac
     3601 ccttggtgac tatccgaagg cgatcgaagc gcttgaagaa tcggtacgcg cgcggccgaa
     3661 tctttggttt acccaagcgt ggttggttgc cgcctatgcc ctatccaata aagatgcaga
     3721 ggcgcgcaaa gcgctcggag catttaagaa aacgccattc agcacccgat tcgacctcga
     3781 ttggatcacc caatactaca aggaagagca gtaccaaaat cccacgctac aggcggcttc
     3841 ggcggaggtg ctgaacggcc tacgtaaggc tggcttgaag tagcaacaaa caacgcgaaa
     3901 ctgccaagag agataaaggc gggcttgctt taggccgagc accagcgcaa agccgatgat
     3961 gtggaacagc tgcgggcgct cgccaagaaa cgacgatcgc catggccccg ttgcgcaacg
     4021 agcacgactg atgtcgatgc ggattcgctt tcatgctccg gaaggcaaca gttccagcat
     4081 catgattgtg aaacggatcg tcgcgctgct gacaggctgt cacggaggct cgtcaccacg
     4141 acgaggcgat tgatctcacc gttgttaaag gctttcaaaa atctctcata gtccttgatc
     4201 gacacgcagc cgttagaatc gcctttcgct ccgagcatat agctgtgcgc gagcaatccg
     4261 gaccgcccga gcgtaccttg ggcgccaacc ggaatcatgc ggagcgcttg gacgccgtga
     4321 aacggtcgct cccgcggttt cagatcatag acatttggtg gcgtcgctcc aacatttcgc
     4381 tcgctcacat gacctggatc atccatcaaa ctaccaaatc ctgaatgcgc ctcgagcttc
     4441 aacccgtttg gcatgtaaac agtgcgcgcc gagatatcat agacggccgt gagattgtcg
     4501 tagccaagag acgtgagatc cggccgatcg ccaaggagcc caccatctgg agaaagagac
     4561 gcaaacgtga cacgggccgg gagcagatcg gaaagcttct gcagtagcgt gcgactgtca
     4621 ggttgagccg taagagggag gtctcgtaat tcgacgttcg cctcaactgg acgaggtctc
     4681 ggaaggggaa cggcagcgac gcttgatggc gcgggttcct ccacaggggc tacccgccag
     4741 tctcgagact gcagcttctg cgcgagccgt tctttcgctt gttgaaattt gatctcgagt
     4801 tctgaaggca aagattgaac aacgggtagc gggaagtacg tcggaagcaa attggatcct
     4861 gaatccgaga ggaaccggtc atcgaacgat gacgcgttga tttctttgga ggaaacggaa
     4921 tccgagaccc aagtaaccgg ataggcagtc caggcggcca ctccggatgc caacgcaacg
     4981 atgaccaatg caatcgggcc ggagaacgaa ataccgtgac ccgacggaat cttctcagct
     5041 gtcgctgcga cgtaggtcat ccagtacacc cgtcggacgc tttgcaaccg agccaacatc
     5101 gtggttgttg gaatacggcg aaatcgaggc aaatgccggc agcttgagat cttcgcggcg
     5161 agtagtgaca caatcgccac actcagatga attgtgcgat tgatacccag ccatttcatc
     5221 tggccatggt gccgcttatt gcgttccgcc cctgttacct gttggatttc ggcaggttgg
     5281 gggtacgagt tgggggtacg acatgtcttc catcagctgt gccagaactt cgcacgtcga
     5341 cattagagca aaccgttttc tgcttaacgc gcgtttggtc tcggccgctg gtgttcggaa
     5401 cgtcattcag ctgtttgccg tcgctctggt cgcggcttcg cttgcggcct gcgcccagtc
     5461 ctctgtggtc accagaaaat ctgaattact tactgccagt cgacaggcat cgttggaaca
     5521 gaatcgaaca acatcgttcg tgacgaatag gcgtgtcgcg gtcacgagaa agcatacccc
     5581 gttcgcgtca cacaaaaatg cagccgagac gcaggttgcg tcgcatggac ttgccagctt
     5641 ctacacggaa ggaacacaga ccgcgaacgg tgaaaagttc gatacgcatg aattgaccgc
     5701 cgctcatcgt acgttgccat tcggtacccg gttgcgcgtg acaaacgtcg ctactgggcg
     5761 gtccgtcact gttcgggtca atgaccgcgg tccgttcatt cccggacgcg ttgtcgacgt
     5821 ctcgtattct gcggcgcaaa cgctgggaat ggttggaggc ggtattgcaa aggtcaaact
     5881 tgacgtcgtc caatagtagc ggaagcggcc cagacgtaac gatcatcttg gtcgattctg
     5941 acgaccggcg cgtcctaaat ggcatctttg ggcgatgcgc ccaggcgcac cccggcgcga
     6001 tccgccggag accatggact ccgcaaaacc tgctgcaacc gctccgttcg ttggctaatg
     6061 cacattcgcc ggcttgcgcg acgcgacgaa cacgccggtc agcaccagcg caaagccgat
     6121 gatgtggaac agctgcgggc gctcgccaag aaagacgatc gccatcgccg atccgaacac
     6181 cggcacgaca tgaaagaatg gcgcggcgcg gttggcgccg atcagctgca cgccgcgatt
     6241 gaagcacaga taggccagcg ccgaaggaaa caccgcgacg taaaacagcg acagcaggtt
     6301 cgcggtgttc agctgcatca ccgggcgggc gaacaattcc catatccaca gcggaaccag
     6361 gcacaaagcg ccacagccaa aggtgaaggc gacaaacgac aggccgtgaa ttgccggacg
     6421 cttcagcgac agcaccgaat acagcccgaa gatcgccagc gccacggtga agatgatatc
     6481 gcccttgttg aacgcgatgc tggtgagcgc cgtcaggtcg ccgtgcagca ggatcaccag
     6541 cacgccggtc agcgacagcg cgaccccgcc ggtctgcgcc agcgtcaagc ggacaccaaa
     6601 caggaccagc gaccacaccg cgacgatcag cggtccggct gactgcagca gcagcgtgtt
     6661 cagcgccgtg gtgtgttcga gcgaccagta ttgcaaggtg ctgaaggcgc tgatcccgat
     6721 gacggagaga aagatcatgg tgccgagatg gctgcggatc gccccccggt cgcgcgcaag
     6781 gtgcttccag gcgaagggga gaatgacaag caacgccaac gaccagcgca ggaacgacag
     6841 cgtcaccggc ggaatatgcc cggcggcgag gcggccgacg atggcgttgc cggcccagca
     6901 caacgcggtg atcgagagca gcagataggg ctgattggcg agccaattgc cggaggggac
     6961 ggcgctggag gtgtgatcgg aggctgacat gcggcggtcg ttcttggccc ggttccatga
     7021 cgccgcggcc cggcccggtg ttccccaccg ggccggaatc atggcccggc cgcctcacag
     7081 acacggattc tcaccgcgca tcaatggcgt ggatgcatcg cgaccatgca caaggaggca
     7141 tttcgcggct cgggtccgct caacaccggt tggaaatcgc cggcgccatc gtctatcgtg
     7201 cggcgatgtt gcaaaagttc atcttcgctt tggcgatcgg cgcgggctgt gccagcgtcg
     7261 cgatggccga gccgcgctcc ggcatcgata tcccggcgat cgatccgagc gtgcggccgc
     7321 aggacgattt ctggcaattc gccaacggca aatggctggc ggcgacgtcg attccagccg
     7381 accgcgccgc ctgggacacc ttctccgcgc tgcgcgaaac caaccagcag cagttgcgcg
     7441 acgtgatcga agcgatcgat ccgcgcagcc tggacggcgg cgagccgcgc aagctcgccg
     7501 atttctatgg ctcgttcatg gacgaggcgc gggtggaagc gacgggcctg gacggcttgc
     7561 gtgacgaact gcggcggatc cacgcgttga gcgacaaggc ggctctgccc gaattgttcg
     7621 cgcatctttc ccgactttcg gtgcgcatcc cctgggggct ggagatcgga ccggatgagc
     7681 gcgatgcgac gcgctatgtc gcgcatctgg accagagccg tttgggattg ccggatcgcg
     7741 attactatct caaggacgac gcgcatttcc aggcgatccg gaccggctat cgcgaccaca
     7801 tcgtcaaatt gctggcgctc gccggcgaac cggcggcgga gaccagcgcc ggcgagatca
     7861 tcgcgctcga gaccaggctg gcgcgcctgc agtggacgcg ggtcgagaac cgcgatccgc
     7921 tcaagaccta caacaagcgc gatatcgcgg cgctgccggc gctgacgaca aaagacgact
     7981 ggccgtcgtt tctttccgca ggcggcgtcg gaaccgaaat tcgcagcgtt gtcgtcgcgc
     8041 agccgagcta tttcgacggc gtcggcgaaa ttcttcgcga ggtgccgctg gcaacctggc
     8101 aggcttacct cgcctacaac ctgctcagcg cctatgcgcc ctatctttcg gcgccgttcg
     8161 tcgccgagga atttgccttc gagcagcaca cgctgcgcgg cgtgccggaa atccagccgc
     8221 gctggaaacg cgccgtcgcc atggtcgatc gcttgaccgg ctttgcgctc ggccggctct
     8281 atgtcgaacg ctattttccg cccgccaaca aggcgcgggc cgacgccatg attgcaaatc
     8341 tgacggcggc ctatcgcgag agcattgcga cactcgactg gatgggcccc gagacacgtc
     8401 gcgaggcgct cgccaagctt aaggccatcg ttccgaaaat cgcgtacccg acgatatggc
     8461 gcgactacag cggccttgtc atccggcgcg acgacctggt cggcaatgtc atgcgcgcgc
     8521 cgcttcaact acgatttcca gctgggcaag ctcggccacg aagtcgaccg cgcggaatgg
     8581 ctcaccacgc cgcagacggt caacgcatat tacagcgcca gccgcaacga gattgcgttt
     8641 cccgccggca tcctgcagcc gccgttcttc gacgccgagg cagacgacgc cgccaattac
     8701 ggcgccatcg gctcggtgat cggccacgag atcagtcatg ccttcgacga ccatggcagc
     8761 cggttcgatg gcgacggcaa tttgcgcaac tggtggaccg aagacgatcg tactcgcttc
     8821 gaaggcaaaa cccgcagcct ggtggcgcag tacgacgcct tcagcccgct gcccggtcac
     8881 cacgtcaatg gcgcgctgac gctgggggag aacgtcgccg ataatgcggg ccttgcgatc
     8941 gccgagcggg cctatcgcct ttctctcggc ggctgccccg cgccggacat cgacggctat
     9001 accggcgagc aacgcctgtt catcagtttc gcccaaatct ggcgcgacaa ggtgcgggag
     9061 gccgcccgca tcgagcgtct caaggtcgac ccgcattcgc ccggacagtt ccgcgccgat
     9121 ggcacgctgc gcaaccagag tgcctttgcc gatgccttcg cggtcaagcc gggcgacgcg
     9181 atgtatctgc cgccggaaca gcgcatatcg ctgtggcaga ccgacagtga agtggtgtgc
     9241 aaggaaaagg cgcgttagcg ccgcgcgtcg ggcgacgccc ttcgacctct ggaattcgct
     9301 agagcatgat ggctttgagt tggatcggcg ctgacgttgg ttacctcgcc ccgcttgcgg
     9361 ggagaggtcg gattgcctcg gcgatgcgaa gcatcgtccg gtgcaatccg ggtgaggggg
     9421 ctatccgcga gtccgagcct gtcgagagtc cccccctctc cccgcaagcg gcagtgctgt
     9481 cccgggaaaa gtcacacata gatgagctcc agctgatcga gggagaattt tggttttggc
     9541 ttgctgggat tgactggcgg aggcttgtcg atttctgcga tcggaattcg cgtgaaaagg
//
LOCUS       DASWGM010000381         6829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_304817,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000381 DASWGM010000000
VERSION     DASWGM010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304817"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGA15_11060"
     CDS             complement(<1..384)
                     /locus_tag="VGA15_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588266.1"
                     /translation="MFRETWRSDLTSRKLFWIALLAALVSILPVMSGPATAEGLNPQQ
                     QLAFDIYKELVEINTVTATGDTAQAAEAMAARLRAAGFAEADVHVFSPVPRKGNLVAR
                     LRGTGARKPILLVAHLDVVPASREDW"
     gene            complement(479..982)
                     /locus_tag="VGA15_11065"
     CDS             complement(479..982)
                     /locus_tag="VGA15_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9588267.1"
                     /translation="MKPGAILTLEALLTGALIASLNAAQADVVRYPIPNSTFPISQAV
                     QVPGNATTYYISGQVPPVISKEADPSSPQAYGDTKTQTVGVLNKIKAILEGFGLGMAD
                     VVKMQVFLVHDARAPMDFKAFMEGYTQFFGGSQPNLPARSVVGVAALANAGFLVEIEV
                     VAAKDNK"
     gene            complement(1015..1449)
                     /locus_tag="VGA15_11070"
     CDS             complement(1015..1449)
                     /locus_tag="VGA15_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9588268.1"
                     /translation="MTQGNANLRAAFVMTISAALLLSSFMAYAQSAEPSTSRPALSTG
                     FRFIETSGEELFANVCRGCHMPDGKGAVGAGTYPSLVANKNLEAGGYPVDLVVNGQRA
                     MPPFGAMMSDDQVAAVVNYLRTHFDNNYQDAVTAADVKTVRR"
     gene            complement(1491..3086)
                     /locus_tag="VGA15_11075"
     CDS             complement(1491..3086)
                     /locus_tag="VGA15_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9588269.1"
                     /translation="MSSEPGTVSRRDLLSLIGAVSGSAAMYHAMTSLGFAADSGYKGP
                     IKLDGDPRGASVLILGAGLAGMTAALELRKAGYNVRILEFNSRAGGRNWTLRGGDSFT
                     ELGGFKQTCEFEQGLYINPGPWRIPYHHRALLDYCKRLGVTLEPFIQLNHNALLHATK
                     AFGGMPQRIRDIKTDFQGQISELLAKATQQGRLDEAVSKEDREILMQALRSWGALDGN
                     YAYKANLISADFRGYAKDPGGGIGAAPIAGEPINLSDILKSRVWRYLQNFALHTFQTT
                     MFQPVGGMDMIGKAFAREVGDVIRYEAKVTAIQQDDHGVTVTYTDLKAPATPQQAKAD
                     WCVCTIPLSILSQLPINVSDRMKAAIDAVPYSPSVKIGLQFKRRFWEEDEAIYGGISY
                     TDLPIRQIAYPNTGFNSSAKGVLLGAYLFDGPNAYEFTAMPPAERIARAVEFGAGIHP
                     QYRAEYENGVAVAWHRMPFTLGCAGNWTDKARAEHYDDLCQIDGRIVLAGEHASYIPA
                     WQEGAILSSLNAITRLHDRVIKT"
     gene            3370..4722
                     /locus_tag="VGA15_11080"
     CDS             3370..4722
                     /locus_tag="VGA15_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9588270.1"
                     /translation="MADPNARSAGNSGDGVLELLRLFKGNDAAADRYATTCREMAETI
                     EPVLRAFEYFAEDVARKPGPLSGIPVGIKDIIATADMPTTNGSPVYKDCIPGADAWVV
                     ERLRNLGATIFGKTVSTEFAWRHPGPTVNPWNPKHTPGGSSSGSAAAVAAGVIPLALG
                     TQTLGSVIRPAAFNGVVGLKPSYGAIPRTGVHPLSPSLDHVGFFARRVDDVAYALSLL
                     GGSSDSDVHGRPLPSFQVSVEQGLPPLAAPRLAVVRFAKWSKAEPEQQQLFDTAVATL
                     RDAGAIVQELELPELDQANWDAINTILASEAALIFGDLVERFPDRTSDRLKELVRTGK
                     AQSAYNYLAAKALQEKLRSAFSAQMSGFDAVLTLPAFGEAPEGLGYTGDAEYCAPWTL
                     LGVPAVSLPAGFGKNHLPLGLQIVGAYREDYRMLRVAKWIEGVLMFDMGIPSIDRTEV
                     "
     gene            4813..>6829
                     /locus_tag="VGA15_11085"
     CDS             4813..>6829
                     /locus_tag="VGA15_11085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX9588271.1"
                     /translation="MIAARPQRETFTKDSPGIARAVRHLAAGLAATVVLALLLSGKAV
                     AEPGYSFDTTPGKLPKAVIPLHYAIELTPDLASLKLEGVEAVDIEVREPTARLTLNAV
                     DMTLGAATIDDDAQRADVALDATTETATLTFPKALAAGPHRLRIGFTARINAFGRGLF
                     FVDYPTDNGVKRMLSSQLEPADARRIFPCWDEPAFKATFALTVTVPRTFLAVGNMPVA
                     HEEPVAPDKKQVAFAPTPKMSSYLFVFAAGELERLTAETNGVTVGVVTTAGKREQGRF
                     ALENAVKLLDYYNDYFGIKYPLPKLDLIAVPGGFGGAMENWGGITFFESRLLFDPSTN
                     ADTARRGIFSVLAHEMAHQWFGDLVTMGWWDNLWLNEGFASWMQEKAAEHFYPQWQTW
                     LNGYGQKQYAMALDARRTSHPIQQPVADHSEANAAFDGITYNKGQALIRMLENYLGER
                     AFRSGIGKYMAAHAYGNTTTADLWQALESAAGKPVTGIAASFTEQDGVPLIVAETSCN
                     SDTQQMTLRQDRFVIIPAASSMAGDGATSLPLRSWQVPIALGPPRSTRPTEVLLLNGS
                     SEIAAGSCGEAVKVNLGDVGYYRTEYGPNSRAALAKSLALMTPGDRVNFLADSWALVQ
                     AGRAEPPSYLALIEEISRDDRRAVWDQVIGTLTTLDRLARDRAERPAL"
BASE COUNT         1304 a         2136 c         2089 g         1300 t
ORIGIN      
        1 ccagtcctcg cggctggccg gcacgacgtc gagatgcgcc accagcagga tcggcttgcg
       61 cgcgccggtg ccgcgcaggc gcgcgaccag attgcccttg cgcggcaccg gcgagaacac
      121 gtggacgtcg gcctccgcaa aacccgcggc gcgcaggcgg gccgccatcg cctccgccgc
      181 ttgcgcggtg tcgccggtcg cggtcacggt gttgatctcg acaagctcct tgtagatgtc
      241 aaaggcgagc tgctgctgcg ggttcagccc ttcggcggtc gctggccccg acatcacggg
      301 gaggatcgag accagggcgg cgaggagagc gatccaaaaa agcttccgcg aggtgaggtc
      361 gctacgccag gtttctcgaa acatcccgct ctcccaaaaa agcagccgga tgaattcaga
      421 caggtaacgc gttcgctggc aagctatcga accaaccact tctgcggctc agctatccct
      481 atttgttatc tttggcagcg accacttcga tttcgaccag aaaaccggca ttggcgagtg
      541 ctgcgacgcc gacaaccgac cgtgccggca gattgggctg actgccgccg aagaactgcg
      601 tgtaaccctc catgaatgcc ttgaaatcca tcggcgcgcg cgcgtcgtga acaagaaaca
      661 cctgcatctt gaccacgtcg gccatgccga gcccaaaacc ttcgaggatg gccttgatct
      721 tgttgagaac accaaccgtc tgggttttgg tgtcgccata ggcctgaggg ctggacgggt
      781 cggcctcctt gctgatgacg ggaggcacct ggccgctgat ataataggtc gtcgcgttgc
      841 ccgggacctg cacggcctgg gaaatcggaa aggtcgagtt cggaatggga tatctgacca
      901 cgtcggcctg cgcggcgttg agactggcaa tgagagcgcc ggtcaaaagc gcttcgagtg
      961 tcagtattgc tcccggcttc atctgcctct ccgcgtgttg gatgagaatg atcgttatcg
     1021 gcgaacggtt tttacatccg cggccgtcac cgcatcctga taattattgt cgaaatgggt
     1081 ccgcaggtaa ttcaccacgg cggcgacctg atcgtcgctc atcatcgctc cgaacggcgg
     1141 cattgcgcgc tgtccgttga cgacgaggtc gacgggatac ccgccggctt cgagattctt
     1201 gttggccacc agcgatggat aggttccggc gcccaccgcc cccttgccgt cgggcatgtg
     1261 gcagccccga cagacattgg cgaacaattc ctcgccgctg gtttcaatga acctgaagcc
     1321 ggtgctcagg gctggcctgg atgtcgaggg ctcggcgctc tgcgcgtaag ccatgaacga
     1381 tgacagcaag agtgctgccg atatcgtcat gacaaacgct gcgcgaagat ttgcgttgcc
     1441 ctgcgtcatc cggacaaatg cccgcggaat tctgttttga aaacgtcgca tcacgtcttg
     1501 attacccgat catgcagccg ggtgatggcg ttgagagatg aaaggatggc tccctcctgc
     1561 catgccggaa tgtaggatgc atgctcaccc gccaacacga tcctgccgtc gatctggcat
     1621 agatcgtcgt agtgctccgc ccttgccttg tcggtccagt tgccggcgca gcccaacgta
     1681 aacggcatgc ggtgccaggc aacggcaaca ccattctcgt attcggcgcg gtattgcgga
     1741 tgaatgccgg cgccgaattc gacggcccgc gcgatccgct cggctggcgg catcgcggtg
     1801 aattcgtagg cgttgggacc gtcgaacaga tacgcgccca gcagcacgcc cttggccgag
     1861 ctgttgaagc ccgtgttggg ataggcgatc tggcgaatcg gcaggtcggt gtagctgatg
     1921 ccgccataga tcgcctcgtc ctcttcccag aagcgtcgtt tgaactgcag gccgatcttg
     1981 accgacggcg aatagggcac ggcgtcgatg gctgctttca tccgatcgct cacattgatc
     2041 ggaagctggc tcagaatcga gagcggaatg gtgcagacgc accagtcggc cttggcctgt
     2101 tgcggcgtcg ccggcgcctt gaggtcggtg taggtgaccg tgacgccgtg gtcatcctgc
     2161 tggatcgccg tcaccttcgc ctcgtaacga atgacgtcgc caacttccct ggcaaacgcc
     2221 ttgccgatca tgtccatgcc gccgaccggc tgaaacatcg tggtctggaa ggtatgcagc
     2281 gcgaaattct gcagataacg ccagacccgc gatttgagga tatcagacag attgatcggc
     2341 tcgccggcga tcggcgccgc gccgatgccg ccgcccggat ccttggcgta accgcgaaag
     2401 tcagcggaaa tgaggttggc cttgtaggcg tagttgccat ccagcgcgcc ccatgacctc
     2461 aacgcctgca tcaggatctc gcgatcctct ttcgagaccg cttcgtccag tctgccctgc
     2521 tgcgttgcct tggcaagcag ttccgaaatc tgtccctgaa agtcggtctt gatgtcgcga
     2581 atgcgctgcg gcatgccgcc gaacgccttg gtcgcatgca ggagggcatt gtgattaagc
     2641 tggatgaacg gctcgagcgt aacaccgagg cgcttgcagt aatcgagcag cgcgcgatgg
     2701 tgatagggaa tccgccatgg ccccggattg atatacagcc cttgctcgaa ctcgcaggtt
     2761 tgcttgaagc caccgagttc ggtgaacgaa tccccgcccc gcagggtcca gttgcggccc
     2821 cctgcccggc tgttgaattc cagaatccgg acattgtatc cggccttgcg caactccagc
     2881 gcagccgtca tgccggcaag gccggcgccg agaatgagaa cagaagcgcc cctgggatcg
     2941 ccgtcgagct tgatcggacc tttgtatccg gaatcggccg cgaaccccag gctggtcatc
     3001 gcgtgataca tcgcggcgct gccggaaacc gctccgatca gagacagcag atcacgccgg
     3061 ctaacggttc caggctcgct tgacatgatt ccttattacc cgcgaatccg tccggccagt
     3121 catcaaatcc aagtgtcggg acatcgaccc cgaattcagt tcgcccgcaa ccgaaatgcg
     3181 gaagtgccgg ctttaaattc gacgtgtatc atgaccttgc ataacttcgc ggcgggcggg
     3241 tcacacctgc ctaaagcgca ctgaactcct ttcaacacga tgaaaataaa aggtttttta
     3301 tggctgtgct gatggcatac aagcgcgaca tagatcgaac cggcgctggc attgtcaggg
     3361 ctttccgaca tggctgatcc gaatgcgcgc tccgccggca attctggcga cggcgttctc
     3421 gaattgttgc gtctgttcaa aggcaatgat gccgcggctg atcgatacgc aaccacctgc
     3481 cgcgagatgg ctgagactat cgagccggtg ctcagggcat tcgaatattt tgccgaggat
     3541 gttgcgcgca agccggggcc gctgtccggc attcccgtgg gcatcaagga catcatcgcg
     3601 acggcggata tgccgaccac aaacggctcg ccggtttaca aggattgcat tcccggcgcc
     3661 gatgcgtggg tcgtcgaacg gttgcgcaat ctcggcgcca cgatttttgg taaaacggtt
     3721 tcgaccgaat ttgcctggcg ccatccaggg ccaacggtca atccatggaa tccaaaacac
     3781 acgccgggcg gttcgtcgtc gggttcggcc gcggccgtcg cagcaggggt cattccgctc
     3841 gcgctgggca cccagacgct gggctcggtc atcagaccgg cagcgttcaa tggcgtcgtt
     3901 ggcctcaagc cgagttacgg cgcgatcccg cgcaccggcg tgcatccctt aagtccttcg
     3961 ctcgatcatg tcggattttt cgcacgccgt gtggacgacg ttgcctatgc cttgtctctc
     4021 cttggcggca gcagcgacag cgatgttcat ggccggccgc tcccatcatt tcaggtttcc
     4081 gtcgaacagg gattgccgcc gctggcggcg ccgcgtctcg ccgtcgtgcg gtttgcaaaa
     4141 tggtcgaagg ccgaacccga acagcaacag ctatttgaca cggcggtcgc gacattgcgc
     4201 gacgccggcg caatcgtgca ggaactggag ttaccggaac tcgaccaggc caactgggac
     4261 gcgatcaaca cgatcctcgc cagcgaagcc gcactgattt ttggcgacct cgtcgagcgc
     4321 tttcctgacc gcaccagcga tcgtttgaag gagctcgttc gaaccggcaa agcccagtcg
     4381 gcttacaatt acctcgccgc caaggcgctg caggaaaagc tgcgatcggc attctccgcg
     4441 caaatgtcag gtttcgatgc cgtgctgacg ctgccggcgt tcggcgaggc gcccgaaggc
     4501 ctgggctata ccggcgatgc ggaatattgc gcgccctgga ccttgctggg cgtgcccgcc
     4561 gtgtcgctgc ccgccggatt cggcaaaaac catcttccgc taggcctcca gatcgtcggc
     4621 gcctatcggg aagattatcg gatgcttcgt gtggcgaaat ggatcgaggg cgttttgatg
     4681 tttgatatgg gcattccgag cattgatcgc acggaagttt gaacgctgcg ccgggcgcct
     4741 ttttcctcgc aatctccatc ccgttaggat aactcacggc ggccgttaaa acctgcatgc
     4801 aaggccgagc cgatgatcgc cgcgcgcccg caaagagaaa ccttcaccaa ggattcgccc
     4861 gggatagcgc gcgcggtgcg tcacctcgcc gccgggctgg ccgcgaccgt cgtgcttgcc
     4921 ttgttgctgt ccggcaaagc cgtcgccgag cccggctatt ccttcgacac cacgccgggc
     4981 aaactgccga aagcggttat tcccctccac tatgcgatcg agctcacgcc agatctcgcg
     5041 agcctcaagc ttgaaggtgt cgaggccgtg gacatcgagg tgcgcgagcc caccgcacgg
     5101 ctcacgctca atgcggtcga catgacgctc ggcgccgcca ccatcgacga tgacgcgcag
     5161 cgcgctgacg tcgcgcttga tgccacaacc gaaaccgcga cactcacctt tccgaaagcg
     5221 ctcgcggcgg gcccacaccg gctgcgcatc ggtttcacgg cacggatcaa cgcgttcggc
     5281 cgcggtcttt tcttcgtcga ttacccgacc gacaacggcg tgaagcggat gctctcgagc
     5341 cagctcgagc ccgccgatgc gcgacggatc tttccgtgct gggacgagcc tgccttcaag
     5401 gcgacgtttg cgctgacggt cacggtgccc cgcaccttcc tggcagtggg caacatgccg
     5461 gtggcgcacg aggaaccggt cgcgccggac aaaaagcagg tcgcgttcgc accgaccccg
     5521 aaaatgtcga gctatctgtt cgtgttcgcg gccggcgagc tcgaacgtct cacagcggag
     5581 acgaacggcg tcacggtcgg cgtggtcacg acggcaggca agcgcgagca aggccgcttc
     5641 gcgctcgaaa acgcggtcaa gctgcttgat tactacaacg attatttcgg gattaaatac
     5701 ccgctgccaa aactcgacct gatcgcggtg ccgggcgggt tcggcggcgc catggagaac
     5761 tggggcggca tcaccttttt cgagagccgg ctgctgttcg acccgtccac caacgcggat
     5821 acggcgcggc gcggcatctt cagcgtgctg gcgcatgaga tggcgcatca atggttcggc
     5881 gatctcgtca ccatgggctg gtgggacaac ctctggctca acgagggctt tgccagctgg
     5941 atgcaggaga aggccgcgga acatttctat ccgcaatggc agacttggct caacggctac
     6001 ggccagaaac agtatgccat ggcgctcgac gcgcggcgca cctcgcatcc gatccagcag
     6061 ccggtcgccg accacagcga ggccaacgcc gcgttcgacg gcatcaccta caacaagggc
     6121 caggcgctga tccgcatgct cgaaaactat ctcggcgaaa gagcgtttcg cagcggcatc
     6181 ggcaagtaca tggccgcgca cgcctatggc aacaccacca cggccgatct ctggcaggcc
     6241 cttgaaagtg ctgccggcaa accggtgacg ggcattgccg cctccttcac cgagcaggac
     6301 ggagtgccgc tgatcgtggc cgaaaccagc tgcaacagcg acacgcagca gatgacgctg
     6361 cggcaggatc gatttgtgat aattcccgct gcttcctcca tggcgggcga tggagcgacg
     6421 agcctgccac tccggagttg gcaagttccg atcgcgctcg ggccgccgcg ctcgacgcgg
     6481 ccgacggaag tcttgctgct gaacggcagc agcgaaatcg cggctgggtc ctgtggcgag
     6541 gcggtaaaag tcaatctcgg cgacgtcggc tattaccgaa ccgaatacgg ccccaacagc
     6601 agggcagcgc tggcgaagtc gctggcactg atgacgccgg gtgatcgtgt caacttcctc
     6661 gccgacagct gggcgctggt gcaagccggc cgcgccgaac cgccgtctta ccttgcgctg
     6721 atcgaagaga tcagccgcga cgaccgccgc gcggtatggg atcaggtgat cggcaccttg
     6781 accacgctcg atcgtctggc gcgcgatcgc gccgagcgcc cggcgctgc
//
LOCUS       DASWGM010000382         5718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2535828,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000382 DASWGM010000000
VERSION     DASWGM010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535828"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VGA15_11090"
     CDS             complement(<1..570)
                     /locus_tag="VGA15_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9588272.1"
                     /translation="MSGTLGIKAARIAIVLAVFALWEMLSRTGIVNPRLLPSASDTFA
                     MLGELLQRASVRNDLVVTASEVLTAFVLAVPVGTLIGFLIAENRYFAEVTKPLLFFAF
                     SIPKSIFLPMFILVFGVGFPEKVGFGFFSTIFIVIMSTTTAVESVKVEHLTVARSYGA
                     TAAQTAFRVYLPSMLPVLLEALRISMIFNL"
     gene            complement(581..1552)
                     /locus_tag="VGA15_11095"
     CDS             complement(581..1552)
                     /locus_tag="VGA15_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588273.1"
                     /translation="MTTITRRHLLAALTATALLGAPAASYAETVRIGLPTKTYWPTTV
                     AEAAKSQKLFEKEGITAELTIYRGGAETFEAMAAGAADLILDAPSLPAAGRKKGVMSK
                     VVANGAMGNFGWQLMVLAKSPFDVKDLNGKKVAITSAGSGSDLLALWTIQEKKIDFTR
                     VPVGGGGLVPNLLAGNVDAAVVYSPLSFQIAKSGEARTILDYARDVPPSLTSGWIATD
                     KYAKEKPEALQKALNALYGGVMFMRNNREAAIKLIAELYEMPAEIAAAEYENTILKLE
                     TDGNMSAPNVTEALQLSLDMAKLGGMKDLVPAPDIISTGFKPVATRP"
     gene            complement(1705..2373)
                     /locus_tag="VGA15_11100"
     CDS             complement(1705..2373)
                     /locus_tag="VGA15_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9588274.1"
                     /translation="MAEIDTTADRRCRGRPQVRPDDETRQLIYEAARHEFAGNGYAAT
                     SIETVARRAGVSTKTLYRLIPNKAALFEEMVSDRLDRFLSEVNLHTADHADIEEALRA
                     ALMVCADLVLDEEVVALQRMMLQEAGKFSGIAELFYTNGIQRTVVALADWLRVRQARG
                     LIALDDVEEAAGMLLGMVASAPRRAAIFGGRPLPSRPQIEARVRTCAALFLRGCQVQG
                     IGGH"
     gene            2481..3743
                     /locus_tag="VGA15_11105"
     CDS             2481..3743
                     /locus_tag="VGA15_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007637220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX9588275.1"
                     /translation="MTHAATIPVPTAKEPLPDIRDKDSRNEPARPGFALKNLNFRKLL
                     MAGAALVVLAGAVNYGWDYWTVGQYLVSTDDAYVKADSTTVAPKISGYLREVLVGDNE
                     RVKAGEVLARVDERDFRVALDQAKSDVAAANAAIASKQAQLEVQQAVIDAAKATVDVD
                     KATVTFAAQENKRYTDLASSGYGSVQNAQQAQSHIAGAQAAIQRDTANLASALKQVDL
                     LKAEIAQAKAALARASATQSQAELNLGYTTIVAPIDGIIGNRSLRIGQFVQAGTQLMS
                     VVPVSGAYVVANFKETQLTAVREGQPVEIAVDMFPGQRVHGHVDSIAPASGQEFALLP
                     PDNATGNFTKVVQRIPVKIALNRDSNALIELRPGMSVVPTIETHASAPAGKAVAESKL
                     SSNVSGGSCHVKSSPVGLDNRAPLWQQI"
     gene            3685..5301
                     /locus_tag="VGA15_11110"
     CDS             3685..5301
                     /locus_tag="VGA15_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX9588276.1"
                     /translation="MSNPALSASITAPPSGSRSERADVNPNRASTATWIAVLAAMIGS
                     FMAILNIQITNASLLNIEGGIGTGVDNGSWISTSYLIGEIVVIPLTDYLSRVFSFRRY
                     MLASASLFAAFSVACAFAHDLPSMIAMRGLQGFAGGVLIPMAFTMVLTKLPKPQQPIG
                     LAIFALSVTFAPAIGPTIGGYLTENYGWQTIFFVNVLPTIVMVTALYLTLERQPMQLG
                     LLREGDWTGIVTMAIGLSAFQTVLEEGNKDDWFASPFILRLAIVALVSLSLFVWIELT
                     VEKPLIRLRLLKQRNFGFGTISVTLLGFALFGSVYILPAYLGQTQGYNAEQIGAVLAW
                     TGLPQLLLIPLVPKLMQRFDARYIAITGLLIFAYSCFMNTAMSPDYAGDQLWIPNIVR
                     AIGQAMVLTPLTSVTTGGTAPQDAAAASGISNMFRNLGGAIGTAVLATVITKREQFHS
                     NIIGQSVTLGREEVRNRISQTTDFFLAHGVPDPAAARHQAIVALGNAVKHQALVMGFS
                     DTFAVIAAVLVFAAIAVAFTRRVRGGSAAH"
BASE COUNT         1072 a         1887 c         1702 g         1057 t
ORIGIN      
        1 gagattgaag atcatggaaa tccgcagcgc ctccagcagc accggcaaca tgctcggcag
       61 ataaacccga aatgccgtct gcgccgccgt cgccccataa gagcgcgcca ccgtcagatg
      121 ctcgaccttc acggattcga ccgccgtcgt cgtcgacatg atgacaatga agatggtgga
      181 gaagaagccg aagccgactt tctccgggaa gccaaccccg aacaccagga tgaacatcgg
      241 cagaaagatc gattttggaa tactgaaggc aaagaacagc agcggctttg tcacttcggc
      301 gaagtaacgg ttctccgcga tcaaaaagcc gatcagcgtg ccgaccggca cggcgagaac
      361 aaaggcggtc aaaacttcgc tcgccgtcac gaccaggtcg tttcgtacgc tggcgcgctg
      421 caacaactcg ccaagcatgg cgaaggtatc ggacgccgag ggaagcaggc gcggattgac
      481 gatcccggtc cgcgacagca tttcccacag cgcgaacacg gccaggacga tcgcgatgcg
      541 cgccgccttt attccgagcg tgccggacat cgcgcgcgcc tcagggcctg gtcgctaccg
      601 gtttgaaccc ggtcgagatg atatcgggcg ccggcacgag atccttcatg ccgccgagtt
      661 ttgccatatc gagcgaaagc tgcagcgcct ccgtgacatt gggcgcgctc atgttgccat
      721 cggtctcgag cttcagaatc gtgttctcgt attccgccgc ggcgatctcg gccggcattt
      781 cgtacagttc ggcaatcagc ttgatcgcgg cttcgcggtt gttgcgcatg aacatcaccc
      841 cgccatacag cgcgttgagc gccttctgca aggcttccgg tttttccttt gcgtatttgt
      901 cggtcgcgat ccagcctgat gtcaggcttg gcggcacatc cctggcgtag tcgaggatgg
      961 tccgggcctc gcccgatttc gcgatctgaa agctcaacgg cgaatagacc accgcagcgt
     1021 ccacgtttcc ggccagcagg ttaggcacca ggccgccgcc gccgacgggc acgcgggtga
     1081 aatcgatctt cttctcctga atggtccaca gcgccagcag gtccgagccg gatccggccg
     1141 aggtaatggc aaccttcttg ccgttcagat ccttgacgtc gaacggcgac ttcgccagca
     1201 ccatcaactg ccagccgaaa ttgcccatcg caccattggc gacaactttc gacatcacgc
     1261 ctttcttgcg tccggcggcc ggcagcgacg gcgcgtcgag aatgagatcg gccgcacccg
     1321 cggccatcgc ttcgaacgtc tcggcgccgc cacgataaat cgtgagctcg gcggtaatgc
     1381 cctccttctc gaacaatttt tgcgacttcg cggcctccgc cacggtggtc ggccagtagg
     1441 tcttggtcgg caatccgatc cggacggtct ctgcgtagga tgcagccggc gcgccgagca
     1501 gcgcggtcgc ggtcagtgcc gccaggaggt ggcggcgcgt tatggtcgtc attgcgtttc
     1561 ctctattatt ggttcggcgg ttactgcgga gtcttggtgc tcctggccgc tttcgtcagt
     1621 ctggcttgcc tgtctccgat ggaattcagc ctcatcatcg cgcgtcgatg agccgaacac
     1681 aaccgacctg atggccgccg gcaatcagtg gccaccaatt ccctggacct ggcagccccg
     1741 caagaacagc gccgcacagg tgcgtactcg cgcttcgatc tgcggacgcg agggcagcgg
     1801 ccggccgcca aagatggcgg cgcgccgcgg cgcggaggca accatgccga gcagcatgcc
     1861 ggcggcctcc tcgacgtcgt cgagcgcgat caacccccgc gcctgccgca cccgcaacca
     1921 gtccgccagc gccacgaccg tgcgctgaat gccgttggta tagaacaact cggcgatacc
     1981 ggaaaacttg cccgcctcct gcagcatcat gcgctgaagc gcgaccactt cttcgtcaag
     2041 cacaagatcc gcgcacacca tcagcgccgc acgcagcgcc tcttcgatgt ctgcgtgatc
     2101 cgcggtgtgg aggttgacct cggaaaggaa gcggtcgagc cggtccgaca ccatctcttc
     2161 aaacagagcg gccttgttcg ggatcaaacg atacagcgtc ttggtggaga ccccggcccg
     2221 acgcgccacg gtctcaatgc tggtcgcggc atagccgttg ccggcgaatt cgtggcgcgc
     2281 ggcctcgtaa atgagctggc gtgtttcatc gtcggggcgg acctgggggc ggccccggca
     2341 gcgccggtcg gcggtggtgt cgatctcagc catgctttta tatgatgcct gtcattccat
     2401 tgacggatcc aatatggccc ctatttggaa aacatcaagt ttcctaattc gcccttcgta
     2461 gggccaaagg agcaaccgcc atgacccacg ccgccaccat cccggttccc actgccaagg
     2521 aaccgttgcc ggacattcgc gacaaagaca gtagaaacga accggcccgg cccggtttcg
     2581 cattgaaaaa cctgaacttt cgcaaactat tgatggctgg cgccgcgctt gtcgtgctgg
     2641 ccggcgccgt caactacgga tgggactact ggaccgtcgg tcagtatctc gtctccaccg
     2701 acgatgccta tgtgaaggcg gacagcacca ccgtcgcgcc aaaaatctcc ggatatctgc
     2761 gcgaggtgct ggtcggcgac aatgagcgcg tcaaggccgg cgaggtgctg gcgcgggtcg
     2821 acgagcgcga tttcagggtg gcgctcgacc aggccaaatc cgacgtcgcc gcggccaacg
     2881 ccgcgatcgc cagcaaacag gcccagctcg aggttcagca ggcggtgatc gacgccgcca
     2941 aggcgactgt cgacgtcgac aaggcgaccg tgacttttgc cgcgcaggag aacaaacgct
     3001 acaccgatct ggcgtcgagc ggctatggca gcgtgcagaa cgcgcagcag gcgcagtcgc
     3061 acatcgccgg tgcccaggcc gcgatccagc gcgacacggc caaccttgcc tccgccctga
     3121 agcaggttga cctgctgaag gccgagatcg cgcaggcaaa agcggcgctg gcgcgagcct
     3181 ccgccaccca gagccaggcc gaactcaacc tcggttacac caccatcgtc gcgccgatcg
     3241 acggcatcat cggcaaccgc agcttgcgca tcggccagtt cgtgcaggcc gggacgcaat
     3301 tgatgtcggt ggtgcctgtc agcggcgcct atgtggtcgc gaatttcaag gaaacccagc
     3361 tcaccgcggt gcgcgaaggc cagccggtcg aaatcgccgt cgacatgttc cccggccagc
     3421 gcgtgcacgg ccacgtcgac agcattgcgc cggccagcgg ccaggaattc gcgctgctgc
     3481 cgccggacaa cgccaccggc aacttcacca aggtggtgca gcgcatcccg gtgaagatcg
     3541 cgctcaaccg cgacagcaac gctctcatcg aactgcgccc aggcatgtcg gtggtcccga
     3601 ccatcgaaac gcacgcctca gcgcccgcgg gcaaggcagt tgccgagtca aagctctcaa
     3661 gcaacgtttc cggaggatca tgccatgtca aatccagccc tgtcggcctc gataaccgcg
     3721 cccccctctg gcagcagatc tgagcgcgct gacgtgaatc cgaaccgcgc cagcacggcc
     3781 acctggatcg ccgtcctcgc ggccatgatc ggctcgttca tggcgatcct gaacattcag
     3841 atcaccaacg cttccctgct caacatcgaa ggcggcatcg gcaccggcgt cgacaacggc
     3901 tcctggatct cgacctcgta tttgatcggc gagatcgtgg tgatcccgct caccgattat
     3961 ctcagccggg tgttctcgtt ccgccgctat atgctggcga gcgccagcct gttcgcggcg
     4021 ttctcagtag cctgcgcctt cgcccacgat ctgccgtcga tgatcgcgat gcgcggcctg
     4081 cagggattcg ccggcggggt gctgattccc atggcgttca ccatggtgct gaccaagctg
     4141 cccaagccgc agcagccgat cgggcttgcg atctttgcgc tgtccgtcac ctttgcaccc
     4201 gcgatcggcc cgaccatcgg cggttatctc accgagaatt acggctggca gacgatcttc
     4261 tttgtcaacg tgctgccgac catcgtcatg gtgaccgcgc tgtatctgac gctggagcgc
     4321 cagccgatgc agctcggtct gctcagggaa ggcgactgga ccggcatcgt cacgatggcg
     4381 atcggattgt cggcgttcca gaccgtgctc gaggagggca acaaggatga ctggttcgct
     4441 tcccccttca tcctgcgtct ggctatcgtc gcgctggtca gcctctccct gttcgtctgg
     4501 atcgaactga cggttgaaaa gcccttgatc cggctgcggc tcctgaagca gcgcaatttc
     4561 ggcttcggca ccatctcggt gacgctgctc ggcttcgcgc tgttcggatc ggtctatatc
     4621 ctgccggcct atctcggtca gacgcagggc tacaacgccg agcagatcgg cgccgtgctg
     4681 gcctggaccg gcctgccgca gctgctgctg attccgctgg tcccgaagct gatgcagcgc
     4741 ttcgatgccc gttatatcgc catcaccggc ctgctgatct tcgcctatag ctgtttcatg
     4801 aacacggcga tgtcgcccga ctatgccggc gaccagctct ggatccccaa catcgtgcgc
     4861 gccatcggcc aagccatggt actgaccccg ctgacctcgg tgacgactgg cggcaccgcg
     4921 ccgcaggacg ccgcggcggc atccggtatc agcaacatgt tccgcaatct cggcggcgcg
     4981 atcggcaccg cggtgcttgc caccgtgatc accaagcgcg aacaattcca ctccaacatc
     5041 atcggccagt cggtcacgct cggccgcgag gaagtgcgca accggatttc gcagacgacg
     5101 gactttttcc tggcccacgg cgtgccggat ccggcggcag cccggcatca ggccatcgtt
     5161 gcgctcggca acgcggtgaa acaccaggcg ctggtgatgg gttttagcga cacctttgcg
     5221 gtgatcgcgg cggtgctcgt cttcgccgcc atcgcggttg cgttcacgcg ccgggtaaga
     5281 ggcggcagcg cggcgcattg agatcacgcc gacgacggcc tcccatgccg tcgccacggt
     5341 cgatgaaagt gggcactgcc gcggcccgcc acgcgttcca accggcaaat ctgcgactgg
     5401 ctccgatttt gcactacgca tcgtggacag ctgtcttccc gattgaaacg cggtcctggg
     5461 cgatatacct aacctaagtg gcatccccgc gaaactgtgc gcttcccggc catgttgaaa
     5521 ttgcgcgcgc ggctccagac aaagacaggg aacgtcaatg gcaaggaccg acgataaaaa
     5581 cactgatggt gcacgcgcaa gcacttcgac tgctgcgtat ctccggggta cgcggagggc
     5641 taacccgctc aaatacacgc ccggcttctt gtatgtcttg gttttcttca tagtcgggaa
     5701 attaatcttc gcggatcc
//
LOCUS       DASWGM010000383         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_304940,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000383 DASWGM010000000
VERSION     DASWGM010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304940"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGA15_11115"
     CDS             <1..499
                     /locus_tag="VGA15_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9588277.1"
                     /translation="EAGYEGVVDRAQAELQKTGATWIATTDYRTYAMLRWYFKDRLPV
                     LQINERGRFLGFRDPGMDSIRGHTGLYVAREPDNDNPLWASTTAVREPLERVERSWRG
                     IVMDTYALEKLTGWTPELSPPPDSPLFRWRVLAGDFQDLPCSGVRSVAALADDHDARP
                     FPGEF"
     gene            501..1475
                     /locus_tag="VGA15_11120"
     CDS             501..1475
                     /locus_tag="VGA15_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9588278.1"
                     /translation="MLFRIAALAFSVCALALPASAADYPDHAIKMIVPFAAGGGTDVL
                     ARIIAQNLNSKWGQPVVVENQPGASGGIGTRAVMKAPPDGYTLLMASTGALMAVSGSA
                     DADGPFDVNKVLSPIAVAAAPPYLLVVSPTLPVNSTADLIRYAKQKPEGLTFGSSGVG
                     AASHLSGLLFASATGIKMLHIPYKGTGPAVTDLLGGRIDMMFAPGPVVQQFVQSGQLK
                     ALGVTDTQRSKFYPDVPTVADGVPGYESVGWFGLLAPPNTPHAIIKQLNEVIVAAMQT
                     QEFRDHLATLGAEPKPQTPDEFGRYINADVAKWSKLVKDNDVQLPGGK"
BASE COUNT          295 a          470 c          494 g          304 t
ORIGIN      
        1 cgaggcgggt tatgaagggg tcgtggaccg ggcgcaggcc gaattgcaaa agaccggcgc
       61 gacctggatc gcaaccacgg actaccggac ctacgccatg ctgcgctggt atttcaaaga
      121 ccgtctgccg gtgctccaga tcaatgagcg cggacgattt ttgggctttc gcgatcccgg
      181 catggactcg atccgcggtc acaccggtct ttacgtcgcg cgcgagccgg acaatgacaa
      241 tccgttgtgg gcttcaacga cagcggtcag ggagccgctc gaacgggtgg agcgcagctg
      301 gcgtgggatc gtgatggata cctatgcgct ggaaaaactg accggctgga ccccggagct
      361 ttcgccgccg ccggactcgc cgctgtttcg ctggcgcgtg ctggctgggg attttcaaga
      421 cctaccgtgc agcggagttc ggagcgttgc ggctttggcc gacgatcatg acgcgcgccc
      481 tttcccagga gagttttaga atgctttttc ggatcgccgc attggcattc tcggtttgcg
      541 cgctcgcgct gccggcttcc gccgctgact accccgatca cgcgatcaag atgatcgtgc
      601 cgtttgccgc cggcggtggc accgacgtgc tggcgcgcat catcgcgcaa aacctcaaca
      661 gcaaatgggg ccagccggtc gtggtggaaa atcagccggg cgcatcaggc gggatcggca
      721 caagggctgt catgaaagcg ccacccgatg gctacacctt gctgatggca tcgaccggcg
      781 cgctgatggc ggtatctggt agcgccgacg cagacggtcc gttcgacgtc aacaaggtgc
      841 tttctccaat cgccgtcgcc gcggcgccgc cctatctgct tgtcgtcagc ccgaccttgc
      901 cggttaactc gacggccgat ctgattcggt atgcaaagca aaagccggaa ggattgacgt
      961 tcggttcatc gggcgtcgga gcggcgtcac atctgtccgg cctgttgttc gcaagcgcca
     1021 ccggaataaa aatgctgcac atcccctaca aggggaccgg cccggccgtg actgatttgc
     1081 tcggcggtcg tatcgacatg atgtttgcgc cgggaccggt ggtgcagcaa ttcgtacagt
     1141 ccggccaact caaggcattg ggcgtgaccg acacccagcg atcgaaattc tatcccgatg
     1201 ttccgaccgt cgctgacggc gtgcccggct acgaatcggt cggctggttc ggcctgctgg
     1261 cgccaccgaa tacgccgcac gccatcatca agcagctcaa cgaggtcatc gtcgccgcaa
     1321 tgcagacgca ggaatttcgc gaccatctgg cgacgctggg cgccgagccg aagccgcaga
     1381 cgcccgatga gttcggccgc tacatcaacg ccgatgttgc caaatggagc aagctcgtga
     1441 aagacaacga cgtgcaattg ccgggaggaa agtgaatatg cgtcttgcaa cttttgtcga
     1501 tcctcagctt ggcagggaag cgcgtttcgg cattgtccgt ggcgaaagcg ttgtcgacgt
     1561 cgt
//
LOCUS       DASWGM010000384         9765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1143208,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000384 DASWGM010000000
VERSION     DASWGM010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143208"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..317)
                     /locus_tag="VGA15_11125"
     CDS             complement(117..317)
                     /locus_tag="VGA15_11125"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HEX9588279.1"
                     /translation="MFPADVLADVRDPARRHLIDPARYYFRTTPLFETGAEAYAWLND
                     VVCVATGQLTEPGLTYEVFQVG"
     gene            725..1198
                     /locus_tag="VGA15_11130"
     CDS             725..1198
                     /locus_tag="VGA15_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9588280.1"
                     /translation="MEPERFHYDLENCSIKRALDVLGEKWTLLVLREAIYGVRRFDDF
                     ARALKCGRGVLSARLKTLTEAGILKRVEYRESDQRARAEYHLDERGIDLFTTIYALSQ
                     WGDRWMLPPDGPAAKVTDRGSGRPVKVVLTSEPKLKSLSMRDLKIAPGPGAKRIR"
     gene            1334..2596
                     /locus_tag="VGA15_11135"
     CDS             1334..2596
                     /locus_tag="VGA15_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9588281.1"
                     /translation="MDTLKVRDAKDVEQVVRAALASEQPLEIIGHGGKRLIGQPMATN
                     ALMDVSALNAVTAYEPNELIITVQAGAPLADVLSLIDSRNQQFAFEPVNTSQLLGTPD
                     IGTIGGMIGAGLAGPRRIRAGGARDHLLGAHAVSGFGDSFKAGGRVVKNVTGYDLCKL
                     LAGSWGTLAVMTEVTLKVMPRPESERTLMLRGLDDITANRAMTAALGSPFDVSGAAHV
                     PNSAFRAGAEALADPLADPLVDLGSPREAVTLLRLEGITASAIHRAAALAAVLAPFGT
                     AEILQDTASAAVWSSIRDVQPFAANGSLGGWPVWRIVCPPAAGGALGERLARDTGGDV
                     IYDWGGGLIWAALPPKPDAQAALLRQRVEAVGGHATLIRASEQVRRDVDVFHPQQPGL
                     AALGERVRHSFDPKNILNRGRMARGSAT"
     gene            2593..3912
                     /gene="glcF"
                     /locus_tag="VGA15_11140"
     CDS             2593..3912
                     /gene="glcF"
                     /locus_tag="VGA15_11140"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX9588282.1"
                     /translation="MKTEFSLAQLADPDIAEADKILRACVHCGFCTATCPTYVLLGDE
                     LDSPRGRIYLIKEMLEKNRRPTQEVVKHIDRCLSCLACMTTCPSGVNYMHLVDQARAR
                     IEKDYRRPLTERLLRAVLAWVLPRPGLFRFSMRLARFGRPLAALLPASKAASASPTLW
                     RRIRAMLALAPGRLPASGPSGGTVFPALGARRGRVALLQGCAQQVLAPRINQAAINLL
                     TRHGIEVVLVKDEQCCGALTHHLGRDGDALARARANIRVWLEEAGRDGLDAILVTTSG
                     CGTVIKDYGFMLREDRDFAGPAAKISALAKDITEYLGGIDLRRQQQQQSDIVVAYHSA
                     CSLQHGQKITQLPKELLSKSGFVVKDVPESHLCCGSAGTYNILQPDIAGRLRDRKVAN
                     IAMVKPDVIAAGNIGCMVQIAGGTSVPVVHTIELLDWATGGPRPGLS"
     gene            3988..4443
                     /locus_tag="VGA15_11145"
     CDS             3988..4443
                     /locus_tag="VGA15_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588283.1"
                     /translation="MAKGKKKKDKKSKKTKKKVLTAKKKTAKKSSKKAAKKSAKKATK
                     SAKKAAKKSRAAAPKKTAKKAGAKKRSAKKQAAPSMPPAPEAAVPKPKPKPRPAAPKP
                     AAPVATPAPAPAPVPSWAAPSPAASSPAPSWGHSGGSSSPSPSDGEDDN"
     gene            4908..5903
                     /locus_tag="VGA15_11150"
     CDS             4908..5903
                     /locus_tag="VGA15_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX9588284.1"
                     /translation="MKKLVLAAAVLAMTAGSTFAADMAVKAMKAPPPAPFDPWDVAFG
                     SAIMNDYIFRGVTQSNHKPSVAAYFEPRYNVTKDLQLYVGVSGESISFANRAAAEIDV
                     YGGIRPTFGAFAFDIGVWGYLYPGGTCQFGGNNGFDFAGKFQGTECQVNFLLNGNTMK
                     KDVSFFEVYGKGNYTVNDNWQFGVTEFYTPSFLNSGAWGNYSSVTGKYTAPPTIFGSS
                     GVGMYVSGEFGRQWFGTTDSFYGVAAFPNGIHYADYDTWNIGIGFTYKVFTLDLRYSD
                     TDLSKGNCSAFTGAFNASGNTNVTPINPGGTGSNWCGAAGIAKLSADLTAMTNLK"
     gene            complement(6111..7859)
                     /locus_tag="VGA15_11155"
     CDS             complement(6111..7859)
                     /locus_tag="VGA15_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX9588285.1"
                     /translation="MNNIRSTLATVWRIAAPYFRSEDKWAGCGLLAAVIAIELALVGL
                     DVLLNQWNNRFYNALQDKNWDVFVREIGVFTLLATCNVVLVVYQLYLNQWLQIRWRRW
                     MTTRYLGEWLQGANHYRMQLQGDAADNPDQRITDDVKLFVERTLDIGVGLLSSVVTLA
                     SFVIILWGLSAAAPLHVSGREFEIPGYLVWGALIYAIFGTALTQWIGSPLVNLDFQQQ
                     RLEADFRFSLVRVRENSEQIALLRGEPAERQRLSDRFSRVVGNWYAIMSRTKRLKAFT
                     QSYSQAAVIFPYILVAPAYFANKIELGGMMQTASAFDSVQKALSFFVSVYRTLAEWRA
                     VVARLDGFETAIASAATLAGSAESIKMASSTEGDEIDLQQLLVRLPSGTPLVSADHLS
                     IRTSERTLVTGPSGAGKSTLFRAIAGIWPFGNGSIAIPANATLMMLPQRPYFPIGALQ
                     AAIVYPAEADAFSSDQVRDALIAVGLPQLATRLEEEAHWNRMLSLGEQQRLGLARALL
                     HRPQYLFLDEATASLDEPSEAALYGLLEEKLPATTIVSIGHRSTLVAFHQRNVVLVRD
                     GDQFALQSQSEDAKPS"
     gene            8168..>9765
                     /locus_tag="VGA15_11160"
     CDS             8168..>9765
                     /locus_tag="VGA15_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEX9588286.1"
                     /translation="MWNQIYNPLGNATLSTIAAAIPVVTLLVLIASGKVKAHIAAIIA
                     VILTNLIAIFIFTMPAGMSIRASLLGIVAGFFPIGWIVLNVIFLYQMTVATGQFELLK
                     RAVGGVTEDRRLQLLLIAFSFGAFFEGASGFGTPVAITGSVLIGLGFSPLAASGLSLI
                     ANTAPVAYGALGTPIQGLASVTGLDPYILGAMVGRQLPVFSLIVPFWVVWAFAGWKGM
                     KDVWPAILVTGLSFAIPQFVISNYINPWIVDIGASLISMGCLILFLKVWQPKVLWLSP
                     ALRGKDESAATMTAPKPMDKTPLTQRELWTALLPWIIVCIVMLIWGNGAFKAWANSIF
                     TYSYPVPDLHNMINKVPPVAAKPTPEGAVFGFTYLSFTGTGMLIAAIISSALMGFSPA
                     RMIAVYGRTIKLCAISLITISAMLAIGTLTRLSGVDATLGLAFAATGVLYPFFGTLLG
                     WLGVALTGSDTASNILFGNLQKITSEQLGLSSVLMGAANSSGGVMGKMIDAQSIVVAS
                     TATNWYGHESTILRFVFKHSIALA"
BASE COUNT         1833 a         2974 c         3103 g         1855 t
ORIGIN      
        1 ttccaaccct gcatcgctgc gccgaactcg gccgcgtgca cgtccacggc ggcaagctcg
       61 cctttccgga gctgccacgg ttttgagcag tggggccggc ctctatgacc acagcgtcac
      121 ccgacctgga acacctcgta ggtgaggccc ggctcggtta gctgtccggt ggccacgcag
      181 acgacgtcgt tcaaccaggc ataggcttcc gcgccggttt cgaacaacgg agtagtcctg
      241 aaatagtagc gggccgggtc gatcagatgc cgccgcgccg ggtcgcgcac gtctgccagt
      301 acgtccgccg ggaagatggc gcgccctcat gtggattgtg gcaccgtcgt cggtctgcag
      361 gatcgcccga gcgtcgatca ccatcgagcc gtccctgcgg atcaacatcc agtccgccga
      421 tccgggcacg accgcaccac gcaaacgcgg gccggcgaat tcacccccgg aagcggcatt
      481 gaacactcgg cgccccaggg ggccgtcccc tccgtctaac cgggcgccga gcgcagcccg
      541 catcgtgaac aagtgggtca agcccggaag tgccatcgcg tcacctttgc tcatgacaac
      601 gccaccgaac tgttcattgg gtcctcctcg cagatcgaac cagtgctgcc gattgataga
      661 tactgagtac ggacttagaa tgcagaatct aggtctattt gtcaaaccca gctatgactt
      721 ggccatggag ccggaacgat tccattacga cctcgagaac tgctcgatca aacgggcgct
      781 cgacgtgctg ggcgagaaat ggaccctgct ggtgttacgc gaggcgattt acggcgtccg
      841 caggttcgac gatttcgcgc gcgcgctgaa atgcggccgc ggtgtcctga gcgcgcggct
      901 gaagacgctg accgaggccg ggatcctgaa gcgcgtcgaa tacagggaaa gcgaccagcg
      961 cgcgcgcgcc gagtatcatc tcgacgagag gggcatcgat ctgttcacga ccatctatgc
     1021 gctgtcgcag tggggggatc gctggatgct tccgcctgat ggtccggcag caaaagtcac
     1081 ggatcgcggg tcgggacgcc cggtgaaggt cgtcctcacc agcgagccga agctgaaatc
     1141 gctgtcgatg cgcgatctca agatcgcgcc gggacctggc gcgaaacgga tcaggtgaga
     1201 gtttcatggc gattggccgg cgtgcggcgc ggcgtccggc agcccgacgg gcttgttgtc
     1261 aacgtgcgtg tctgcgtcta ctggaccgtc tgaattggcg ggcgatcaac caactggagc
     1321 ctcgtctgat atcgtggata ccctgaaagt acgtgacgcc aaggatgtcg agcaggtggt
     1381 gcgcgccgca cttgccagcg agcagccgct ggagatcatc ggccatggcg gtaagcggct
     1441 gatcggccag ccgatggcga ccaatgcgct aatggatgtg tcggcgctga atgcggtgac
     1501 ggcgtatgag ccgaacgagc tgatcatcac cgtccaggcg ggcgcgccgc tcgccgatgt
     1561 gttgtcgctg atcgactcca ggaatcagca gttcgcgttt gagccggtca acacctcgca
     1621 gctcctgggc acgcccgaca tcgggaccat cggcggcatg atcggcgcgg gccttgcggg
     1681 gccccgccgc atcagggccg gcggcgcccg cgaccatctc ttgggcgcgc atgcggtgtc
     1741 gggtttcggc gacagtttca aggccggcgg ccgggtggtg aagaacgtca ccggttacga
     1801 tctttgcaaa ttgctggcgg gatcctgggg cacgctcgca gtcatgaccg aggtgacgtt
     1861 gaaggtcatg ccaagacccg agagcgaacg cacgctgatg ctgcgcgggc tggatgacat
     1921 caccgccaac cgggcgatga cggcggcgct gggctcgccg tttgatgtgt ccggggcggc
     1981 gcacgtaccg aattcggcgt ttcgggccgg ggcggaggcg ttggccgacc cattggccga
     2041 cccattagtc gacctgggat cgccgcgaga ggccgtcacc ctgttgcggc tggaaggcat
     2101 taccgcctcg gcgatccatc gcgcagccgc gctggccgcg gttctcgcgc cgttcgggac
     2161 ggcggaaatt ctgcaagata ccgcctccgc ggcggtctgg agttcgattc gggacgttca
     2221 accgttcgcc gccaatggtt cgctgggggg atggccggtg tggcggatcg tctgtccgcc
     2281 ggccgcaggc ggcgcgctcg gcgagcggct ggcgcgcgac accggcggcg acgtcatcta
     2341 tgactggggc ggcggcctga tctgggcggc gctgccgccc aaacccgacg cccaggctgc
     2401 gctgctgcgt cagcgcgtcg aggccgtcgg cggccatgcc acattgattc gcgcctccga
     2461 gcaggtacga cgcgacgtcg atgtgtttca tccacagcag cccggccttg ccgcgctcgg
     2521 tgaacgcgtc cgccacagct tcgatccgaa aaatatcctc aatcgcggcc ggatggcgcg
     2581 aggatccgcg acatgaaaac cgaattcagc ctcgcccaac tcgccgatcc cgatatcgcg
     2641 gaagccgaca agatcttgcg cgcgtgcgtc cattgcgggt tctgcaccgc gacctgtccg
     2701 acctacgtgc tgctcggcga cgaactcgat agcccgcgcg gccgaatcta tctcatcaag
     2761 gaaatgctgg aaaagaaccg gcggccgacg caggaggtgg tcaagcacat cgaccgctgc
     2821 ctctcctgcc ttgcctgcat gaccacctgt ccgtcgggcg tgaactacat gcatctcgtc
     2881 gatcaggcgc gggcaaggat cgaaaaagac tatcggaggc cgctgaccga gcggctgctg
     2941 cgtgcggtat tggcatgggt gctgccgcgg cccggcctgt ttcgcttcag catgaggctc
     3001 gcgcggtttg gccggccgct cgccgccttg ctgccggcct ccaaagctgc gtcggcgtcg
     3061 ccgactttat ggcggcgaat cagggcgatg ctggcgctcg ccccgggccg tttgccggca
     3121 tcagggccgt ccggtgggac ggttttcccg gcgctgggcg caaggcgcgg acgcgtcgcg
     3181 ctgctgcagg gctgcgccca gcaggtgctg gcgccgcgaa tcaatcaggc cgcgatcaat
     3241 ctgctgacgc gtcacggcat cgaggtcgtg ctggtcaagg acgagcaatg ttgcggcgcg
     3301 ctgacccacc atctcggacg cgatggcgac gcgctggcgc gcgcgcgggc caacatccgt
     3361 gtctggctcg aggaagccgg gcgagacggt ctcgacgcca tcctggtgac gacgtcgggc
     3421 tgcggcacgg tgatcaagga ctatggtttc atgctgcgcg aggaccgcga tttcgcaggc
     3481 cccgccgcga agatttcggc gctggccaag gacatcaccg agtatctcgg cggcatcgat
     3541 ctgcggcgtc agcagcagca acagagcgat attgttgttg cctatcactc ggcttgttcg
     3601 ctgcagcatg gacagaaaat cacacagctt ccgaaagaat tgctttccaa gagcggattc
     3661 gtggtgaaag atgtacccga gagccatttg tgttgcggtt cggcggggac gtacaacatt
     3721 ctccagcctg acattgcggg cagattgcgc gaccgaaagg tcgccaacat tgcgatggtc
     3781 aagccggacg tgattgccgc gggcaatatt ggatgcatgg tgcagattgc cggcggcacg
     3841 tcagtccccg tggtgcacac aattgagctt ctcgattggg cgacaggcgg tccccggcca
     3901 ggattgagct gatcgattgg ccccgcgcgg aagcggatga ctgaagcgct cgatagacgt
     3961 tggaccagcg acaacaggag gatcacgatg gctaaaggga aaaagaaaaa ggacaagaag
     4021 agtaagaaga ccaagaaaaa ggttctgacc gcgaagaaga aaaccgcgaa gaaatcttcg
     4081 aagaaggctg ccaagaaatc ggccaaaaag gccacgaagt ctgcgaagaa agccgccaaa
     4141 aagtccaggg ctgcggcccc gaaaaaaaca gccaaaaagg ccggcgccaa gaagcgttcg
     4201 gcgaaaaagc aggcggctcc ttcgatgcca cccgctccgg aagctgccgt gcccaagccc
     4261 aagcccaagc cgaggcccgc ggcgcccaag cctgcggcgc ctgtggctac gccagcccct
     4321 gcgcccgccc cggtcccaag ctgggccgct cccagtcccg ccgcctcaag tcccgcaccg
     4381 tcgtgggggc actcaggcgg ttcgtcgagc ccgtcgcctt cggacggcga agacgacaac
     4441 tagccaagcg cgtcccggtt accggggtca cgttcaaagg ccaaggccag aaggccgcag
     4501 catccgtcgc tgcggccttt cttgtggggg cgcccaaact tgtgggggcg cccaaacctt
     4561 gagcccaagg atgcaatccg gactgctgca atcccggact tcgccgacgt ttgattcggt
     4621 gggatcggtc gatggcggcc gaactgtcgt tcgtcgcctg tgtctcaaat gaccgttaga
     4681 cgacttaaaa atcggtcaaa attgttgtcg gaatgcaaca cccgctgaca acattccacg
     4741 gaacggccat aacgcctaaa aagtcggcag atgcttgtct ttttcgcttc actgatcggc
     4801 aagcacgttc caaattgtgg tcaggttacg aaatttggtt gcagccggtt atcgcttctc
     4861 cgcgggggcg tcggaactgg gctgttttac atgtgatggg gatcgtcatg aagaaactgg
     4921 ttttggccgc agcggtactt gcgatgactg cgggttccac ctttgcggcc gatatggctg
     4981 tgaaggctat gaaggcgccg ccgccggcac cgtttgatcc ctgggatgtc gccttcggca
     5041 gcgcgatcat gaacgactac atcttccgcg gcgtcaccca gtccaatcac aagccgtcgg
     5101 tcgcggccta ttttgagccc cgctacaacg tgaccaagga cctgcagctc tacgtcggcg
     5161 tctccggcga gagcatttcc ttcgccaacc gcgccgcggc cgaaatcgac gtctatggcg
     5221 gtattcgccc gaccttcggt gcatttgcct tcgacatcgg cgtctggggc tacctgtatc
     5281 cgggtggaac ctgccaattt ggcggtaaca acgggtttga tttcgcaggg aagtttcaag
     5341 gcacggagtg ccaagtcaac ttcctgctca atggtaacac catgaagaag gacgtcagct
     5401 ttttcgaggt ctacggaaag ggcaactaca ccgtcaatga caactggcag ttcggcgtga
     5461 ccgagttcta cacgccgagt ttcctgaact caggcgcctg gggcaactat tcgtcggtca
     5521 ccggcaaata caccgccccg cctacgatct tcggcagcag cggcgttggc atgtatgtct
     5581 ccggtgagtt cgggcggcaa tggtttggaa ccaccgacag cttctacggt gtcgcggcgt
     5641 tcccgaatgg catccattat gctgactacg acacctggaa catcggcatc ggttttacct
     5701 acaaggtgtt cacgctggac ctgcgctact ccgacaccga cctgtcgaag ggtaactgca
     5761 gcgccttcac gggtgccttc aacgcttccg gaaacaccaa cgtcactccg atcaatccgg
     5821 gcgggacagg ttcgaactgg tgcggcgcgg ctggcatcgc caagctctcg gccgacctga
     5881 ccgcgatgac caacctgaag taagtcattc tctcgagact tttgggggcg gcagagcgat
     5941 ctgccgcccc ttttgtttgt ggtgatccga atgaggctgg actggctgca ggcggcgaca
     6001 gatgccatcc gccgccacga tcccggacta tcgcggcggg agccgctgct ggtccttggc
     6061 gcggctctgg cgatcggcgg tgtggcgtgg ctctggccca cgacgtcacg tcaggatggc
     6121 ttggcgtcct cgctttggct ctgcaacgcg aactggtcgc cgtcgcggac cagcacgacg
     6181 ttgcgctggt ggaaggccac gagcgtggag cgatgaccga tcgagacgat ggtggttgcc
     6241 ggcaatttct cctcgagcag accatacagc gccgcctcgg agggttcatc cagcgacgcg
     6301 gtcgcctcgt cgaggaacag atattgcggc ctatgcagta gcgcgcgcgc gagtccgagg
     6361 cgctgctgct cgccgagcga gagcattcgg ttccagtgcg cttcctcctc gagccgcgtc
     6421 gcgagctgag gcaggcctac cgcgatgagg gcgtcgcgga cttgatccga gctgaacgcg
     6481 tcggcttccg caggatagac gatcgcagcc tgcagtgctc cgattggaaa atatggccgc
     6541 tgtggcagca tcatcagcgt ggcgttggca gggatcgcaa tcgagccgtt tccgaatggc
     6601 cagataccgg cgatggcgcg aaacagcgtc gatttgccgg cgccggacgg cccggtgacc
     6661 agggtgcgct cacttgtgcg aatgctcaga tggtcggcgg agaccaacgg cgtgccactg
     6721 ggcaggcgga cgagaagctg ttgcaggtcg atttcatcgc cttcggtcga tgatgccatc
     6781 ttgatcgatt cggcgctgcc cgccagcgtg gcggcgctgg cgatcgcggt ttcaaatccg
     6841 tcgagacggg caacgacggc ccgccattcc gccagcgtgc ggtaaaccga aacgaaaaat
     6901 gacaacgctt tttggacgct gtcgaacgcg gatgccgttt gcatcatgcc gccaagctca
     6961 attttgttgg cgaaataggc tggcgccacc agaatgtagg ggaagattac cgcggcctga
     7021 gaatagcttt gggtaaatgc tttcagccgc ttggtccggc tcatgatggc gtaccagttt
     7081 ccgacgacgc ggctgaagcg gtccgacagc cgctggcgtt cggcgggctc gccgcgcaac
     7141 agcgctatct gttcggagtt ttcgcgcacc cgcaccaggc tgaaacggaa atcggcctcg
     7201 aggcgctgct gttgaaaatc gaggttgacc agtggcgagc cgatccattg cgtcagcgcc
     7261 gttccgaaga tggcgtagat cagcgcgccc cacacgaggt agccggggat ttcgaactcg
     7321 cggcctgaaa catgcaacgg cgctgccgcg gagagacccc acaggatgat gacgaaagac
     7381 gccagcgtca ccactgagct cagcaggccg acgccaatat ccagcgttcg ctcgacgaac
     7441 agcttgacgt cgtcggtgat acgttggtcc gggttgtcgg cggcatcgcc ctggagctgc
     7501 atccgatagt ggttggcacc ctgaagccac tcgccgagat agcgggtggt catccagcgc
     7561 cgccatcgga tctgaagcca ctgattgaga taaagttgat agacgacaag cacgacgttg
     7621 cacgtcgcca gaagtgtaaa gacgcctatt tccctgacga aaacgtccca attcttatcc
     7681 tgaagcgcat tgtaaaagcg gttgttccac tggttcaaga gaacgtcgag gccgaccagc
     7741 gcaagctcaa tggcgatcac cgccgcgagc aggccgcagc cggcccattt gtcttcggag
     7801 cggaaataag gagcggcaat ccgccataca gtcgcgagcg tcgagcggat gttgttcaca
     7861 gattgtcatc tccggggaag gggcttcaaa gccctgaaaa aacggaagaa ttgggagcgt
     7921 ctatactaaa gttgtagatt tcatggtctg cgtcggcttg tcgcgacctg cggctagacc
     7981 ctagcatgcg acctaacggc gaggggcggg ggactctcat acttcgcggg gttgtgagcg
     8041 gattttggtg ctgccgaatt cgcaaggcaa tttcgcgtgt agccagagac gctcccgcca
     8101 tcacgccagg cacacccgac ctcccacgct ggtcgggcgc cgcataatgg cgtggggagg
     8161 aaccgcaatg tggaatcaaa tctataaccc gctcggcaac gcaacgttgt cgaccatcgc
     8221 cgccgctatt cccgtggtca cgcttctggt gttgatcgcc agcggaaaag tgaaggcgca
     8281 catcgcagcc atcattgcgg tgattctgac caacctgatc gcgatcttca tcttcaccat
     8341 gccggcaggc atgtcgatcc gcgcctccct gcttggcatc gtcgccgggt ttttcccgat
     8401 cggctggatc gtgctgaacg tcatctttct ctatcaaatg acagttgcaa ccggccaatt
     8461 cgaactgttg aaacgcgccg tcggcggcgt caccgaggac cggcgtttgc agctgctgct
     8521 gatcgcgttc tcgttcggcg cgttcttcga gggtgcttcg ggatttggta cgccggtcgc
     8581 gatcacgggc tcggttctga tcggcctcgg tttctccccg ctcgctgcct ccggcctgtc
     8641 gctgatcgcc aataccgccc ccgtcgccta tggcgcgctg ggtacgccga tccagggcct
     8701 tgcctcggtc acgggtctcg atccctatat cctcggtgcg atggttggac ggcagttgcc
     8761 ggtcttttcg ctgatcgtgc cgttctgggt ggtgtgggcg ttcgccggct ggaagggcat
     8821 gaaagacgtc tggccagcta tcctggttac cggcctgtcg tttgcgatcc cgcagttcgt
     8881 gatctccaac tacatcaacc cctggatcgt cgacatcggc gcatcgctga tctcgatggg
     8941 ctgcttgatc ctgttcctga aagtctggca gccgaaggtg ctctggctgt cgccggcgct
     9001 gcgcggcaag gacgaatctg ccgcgacgat gacggcaccg aagccgatgg acaaaacgcc
     9061 gctgacgcaa cgcgagcttt ggaccgcatt gctgccatgg atcatcgtct gcatcgtgat
     9121 gctgatctgg ggcaacgggg cgttcaaggc ctgggcaaac tcgatcttta cctacagcta
     9181 tcccgttccc gacctgcaca acatgatcaa caaggtgccg ccggtggccg cgaagccgac
     9241 gccggaaggt gccgtattcg ggtttacgta cctgtcgttc accggaaccg gcatgctgat
     9301 cgccgcgatc atctccagcg ccctgatggg cttctcgccg gccaggatga ttgcggtgta
     9361 tggccgcacc atcaagctgt gcgcgatttc gctgatcacg atctccgcga tgctcgcaat
     9421 cggcaccttg acgcggctgt cgggcgtcga cgcgacactg ggtctcgcat tcgcggcgac
     9481 cggcgtgctg tatcccttct tcggcacatt gctcggctgg ctgggcgtgg cgctgaccgg
     9541 atcggatacg gcgtcgaaca ttctgttcgg caatctgcag aagatcacct cggagcagct
     9601 gggactctcg tcggtgctga tgggcgccgc caactcctcc ggcggcgtga tgggcaagat
     9661 gatcgacgcg cagtcgatcg tggtcgcctc caccgcgacc aactggtacg gacacgaaag
     9721 cacgatcctg cgcttcgtgt tcaagcattc gatcgcgctg gcgtg
//
LOCUS       DASWGM010000385         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1705073,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000385 DASWGM010000000
VERSION     DASWGM010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705073"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VGA15_11165"
     CDS             complement(<1..299)
                     /locus_tag="VGA15_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9588287.1"
                     /translation="MSDTEDTNPGEPPGPSDIRPVSILDEMKRSYLDYAMSVIVARAL
                     PDARDGLKPVHRRILYAMYENGFEWNKPYRKSARTVGDVIGKYHPHGDQSVYDAMV"
     gene            complement(525..926)
                     /locus_tag="VGA15_11170"
     CDS             complement(525..926)
                     /locus_tag="VGA15_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX9588288.1"
                     /translation="MSLAPLLDAAPAIPLHAFGAMTAFVLGVVQFAAPKGTLPHRTIG
                     WIWVCLMAAVAVSSFWIHQIRLVGPWSPIHLLSIFTLVVLPLGVWKAHHHRITDHRRI
                     MIFIFSGALVIAGLFTLLPGRIMHSVVFGPS"
     gene            complement(1112..1576)
                     /locus_tag="VGA15_11175"
     CDS             complement(1112..1576)
                     /locus_tag="VGA15_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX9588289.1"
                     /translation="MGNLGADPEIKRTQDGRPIANLRIATSDTWRDKNTGERKEKTEW
                     HRVVIFSEGLCKIAEQYLKKGAKVYIEGQLQTRKWTDQSGVEKYSTEVVLQNFNSTLT
                     MLDGRSGGGGGNFGSDDSGGGDFGSSGPSSAAPRRAVAAGARNSDMDDDIPF"
BASE COUNT          319 a          491 c          470 g          298 t
ORIGIN      
        1 accatcgcgt catagaccga ctggtcgccg tggggatggt atttaccgat cacgtcgccg
       61 acggtgcgcg ccgatttgcg atacggcttg ttccattcga acccgttctc atacatcgcg
      121 tagagaatgc gccgatgcac cggcttgaga ccgtcgcgcg catccggcag cgcgcgcgcc
      181 acgatcacgc tcatggcgta atcgaggtag gagcgtttca tctcatcgag gatggatacg
      241 ggacgaatat ccgagggtcc cggcggctct ccggggttcg tatcttcagt atcagacaaa
      301 ggggaaatcc ggtcagttct tgatccgaat catatagcgt atcgaaggtg cccaaaccac
      361 ccctaactgg tcccgcgcaa gcggattttc ccttcgtttt tccagagact tacgagcccg
      421 gcgatccccc tttgcggggc ccacggaacc gatggctgga ggggctccgg cggagccggt
      481 ctggcaacga aaaacgcctc acgcctgaca gcgttttttc aaggctatga cggaccaaac
      541 acgacgctgt gcatgatccg ccccggcagc agcgtgaaca gtccggcgat cacaagcgcc
      601 ccggaaaaaa tgaaaatcat gatgcggcga tggtccgtga tccggtgatg gtgcgctttc
      661 cacacgccga gcggaagcac caccagcgtg aagatcgaca gcaggtggat cgggctccac
      721 gggccgacca gccggatctg atggatccag aacgaactga ccgccacggc cgccatcaaa
      781 cacacccaga tccagccgat ggtgcgatga ggcagcgttc ccttcggagc ggcgaactgg
      841 accacgccaa gcacgaatgc ggtcatcgcc ccgaaggcat gaagcgggat cgcgggagcg
      901 gcgtccaaca gcggcgcaag cgacattcga aacaccacat ctgaagcagc gggagcacgg
      961 cccccgaaga gatcaatgta atgagtattt acatttatct ttccagtgtc aagttcaatt
     1021 cgcctcgcgg gtcgccgcgc acgtcatcca cccagccgac ggctcggccg gcggcggtat
     1081 tcgccggccg atgcggaaga atatcgcggg ttcagaacgg gatatcgtcg tccatgtcgc
     1141 tgttgcgggc gccggccgcg accgcgcgac gcggtgcggc gctggacgga ccggaggagc
     1201 cgaaatcgcc gccgccggaa tcgtcggagc cgaaattgcc gccaccgccg ccgctgcgac
     1261 catcgagcat ggtcagcgtg gagttgaaat tctgcaggac gacttcggtc gagtatttct
     1321 cgacaccgct ctggtcggtc catttgcgcg tctgcagctg gccttcgatg taaaccttgg
     1381 cgcccttctt cagatactgc tcggcaatct tgcacaggcc ttcgctgaaa atcaccacgc
     1441 gatgccattc ggttttttcc ttgcgctcgc cggtgttctt gtcccgccag gtgtctgacg
     1501 tcgcgatgcg cagattggcg atcggccgcc catcctgcgt ccgcttgatt tccggatcgg
     1561 ccccgagatt gccaatca
//
LOCUS       DASWGM010000386         3890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2398212,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000386 DASWGM010000000
VERSION     DASWGM010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398212"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VGA15_11180"
     CDS             complement(<1..246)
                     /locus_tag="VGA15_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588290.1"
                     /translation="MPDQSLQGSFPVLNQPIDELALAEIKGAILAKLRLAIGKDAGMA
                     TKHDWYKAAALALRDRIVHHWLTGEKQSYDAGSKRVYY"
     gene            456..689
                     /locus_tag="VGA15_11185"
     CDS             456..689
                     /locus_tag="VGA15_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588291.1"
                     /translation="MRLMIEIVIAALLVSCITATSAAFAAGGKAGRSADGLSCSFSVP
                     ANASPAARCAAIHRQCGGKFFANYCGDKMLSAM"
     gene            complement(758..1051)
                     /locus_tag="VGA15_11190"
     CDS             complement(758..1051)
                     /locus_tag="VGA15_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX9588292.1"
                     /translation="MRGAGTYYVYILASRRHGTLYIGVTNNLRKRLEQHRSGHGSEFV
                     KKYCVHRLVHMEVFASPQEAIAREKQLKDWQRDWKIRLIEQNNLDWSDLSHLL"
     gene            1234..1665
                     /locus_tag="VGA15_11195"
     CDS             1234..1665
                     /locus_tag="VGA15_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588293.1"
                     /translation="MSTIAFDSFALTRIADFARSLSRLHQASRRQPIDDDAIDREFNA
                     VCLSVWGYTTDDFSDELFSAADHAWLDSLDEAHARIFAAEHGYDLVDDNGMLTDWWGY
                     CWMILAEKRGLLTPENRSAARAAIEEKYLTAPNVIGVIVGR"
     gene            complement(1668..2450)
                     /locus_tag="VGA15_11200"
     CDS             complement(1668..2450)
                     /locus_tag="VGA15_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HEX9588294.1"
                     /translation="MRRRAILIATAALTASVALAPRIASAEGLFDFLFGGSPKQQQQK
                     APPQANFFADPFGLNQQAAPPPRPVVSGGSGPAFCVRSCDGKYFPLLARGNATPVAMC
                     QAFCPASVTKVFYGSTIEGASSGNGEHYADSENAFAFRKALRADCTCNGRDPAGLAPV
                     DLTLDASLRQGDMIATTNGLVAYSGVRVGAGQTAEFIPIASYPGLTADVRARLGEMKV
                     APVAAEMIAADAPLPEASRDVALPAALMPKAVSPRAKRAEVE"
     gene            complement(2541..2675)
                     /locus_tag="VGA15_11205"
     CDS             complement(2541..2675)
                     /locus_tag="VGA15_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588295.1"
                     /translation="MNALMALRSIRGTRPALRREAMTNNPNFFSAFSALAHTSFTIPH
                     "
     gene            complement(2838..3401)
                     /gene="pyrE"
                     /locus_tag="VGA15_11210"
     CDS             complement(2838..3401)
                     /gene="pyrE"
                     /locus_tag="VGA15_11210"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX9588296.1"
                     /translation="MSKSASRARLAEIIRKRSFGRGEITLASGRKSDFYFNLKPTMLD
                     PEGASLLAELTYEALKDDNLDYVGGLEMGAVPLAGAIAQLSWIKGHPIAAFFVRKKPK
                     EHGARLAVEGLAKGESLQGKRVVIVEDVTTTGGSALKAVEAVRDAGGEVALVFTMVDR
                     DEGAAETFAQAGLPFRSLYKAGEFLKG"
     gene            3600..>3890
                     /locus_tag="VGA15_11215"
     CDS             3600..>3890
                     /locus_tag="VGA15_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9588297.1"
                     /translation="MTIELHTWNTPNGRKISVALEEMGLPYKVFPINITKGEQMAPAF
                     LAISPNNKIPAIVDPDGPDGKRVSVFESGAILLYLGEKTGKFLPKPLSERIPV"
BASE COUNT          734 a         1208 c         1195 g          753 t
ORIGIN      
        1 gtaataaacc cgcttgctgc cggcgtcgta gctctgcttt tccccggtca gccaatggtg
       61 cacgatgcgg tcgcgcagcg ccagcgccgc cgccttgtac cagtcgtgct tggtggccat
      121 gccggcatcc ttgccgatcg ccagccgcag cttcgcaaga atggcgcctt tgatttcggc
      181 cagcgccagc tcgtcgatcg gctggttgag caccggaaaa ctcccctgca aagattgatc
      241 tggcaaatcg tggatcctgg acctttgagt ctctgccaaa caatacgcgt gttgggccgg
      301 gatcgaaacc cgctttgggg cttcgtttgt gccgtgctcg catggtttat ttatgcaagg
      361 actgggccga tttgccgcaa tctcccatgc gcgccgcttt cgtggtaggg taaaatccgc
      421 cggtgtcggg ataattttga gggaaggacc gggccatgag attgatgatc gagattgtca
      481 tcgcagcact gctggtgtcc tgcatcaccg ccaccagcgc ggccttcgcc gccggaggca
      541 aggcgggccg aagtgccgac ggcctgagct gttcgttcag cgtcccggcc aacgcctcgc
      601 ccgccgcgcg ctgcgcagcc atccaccgac aatgcggcgg aaagtttttc gccaattatt
      661 gcggcgacaa gatgctgtcg gcgatgtgag ttttctgggc ggtaatcgcg cctgctctcg
      721 tcgtcccggc gaaggccggg acccataacc accatgctca aagcaggtgg gacaggtcac
      781 tccagtccag attattctgt tcgatcaaac gaattttcca gtcgcgttgc caatccttca
      841 actgtttctc acgtgcgatt gcttcctgcg gcgatgcgaa aacttccatg tgaacgagac
      901 gatgtacaca gtatttcttg acgaattccg agccatggcc gcttcgatgc tgctcaagcc
      961 gcttccgaag gttattggtg acgccgatgt aaagcgtacc gtgtctgcgg ctggcgagaa
     1021 tgtaaacgta atatgtgcct gctccgcgca tggtttggca cgactttgca agaactctga
     1081 catcggtggc tatgggtccc ggccttcgcc gggacgacgt tagacggcga tcggaacaaa
     1141 ttaagaacac tttagcaagt ctttgatata tcgtagtgat tcggtgcaat gccgatacaa
     1201 catctagggt ctatccgaaa tcttgaggac tacatgtcca ccatcgcctt cgattccttc
     1261 gcgctcaccc gcatcgccga cttcgcccgc tcgctgtcgc ggctgcatca ggcctcccgc
     1321 cgccagccga tcgacgatga tgcgatcgac cgggagttca acgccgtctg cctgtcggtg
     1381 tggggctaca ccacggacga tttcagcgac gagctgtttt ccgccgccga ccacgcctgg
     1441 ctcgattcgc tggacgaggc gcacgcacgc atcttcgccg ccgagcacgg ctacgacctc
     1501 gtcgacgaca acggcatgct gacggactgg tggggctatt gctggatgat cctcgccgaa
     1561 aagcgcgggc tgctgacgcc ggaaaaccgc tccgcggcgc gcgcggccat cgaggaaaaa
     1621 tatctgaccg cgccgaacgt gatcggcgtc atcgtcgggc gttgagttta ttccacttcc
     1681 gcccgctttg ccctcggcga aacagccttc ggcatcaagg ccgcgggcag cgcgacatcg
     1741 cggctcgctt ccggcaacgg cgcgtcagcg gcgatcattt ccgcagccac cggtgctacc
     1801 ttcatctcgc ctaatcgcgc gcggacatcg gcggtcaagc ccggatagga tgcgatcggg
     1861 atgaattccg cagtctggcc ggcgccgacc ctgacgccgg aatacgccac cagcccgttg
     1921 gtggtggcaa tcatgtcgcc ctgacgcagc gaggcgtcga gtgtcaaatc gaccggcgcg
     1981 aggcccgccg ggtcgcggcc gttgcaggtg cagtccgcgc gcagcgcctt gcgaaaggcg
     2041 aaggcgttct cgctgtcggc gtagtgctcg ccattgccgg aagaagcgcc ctcgatggtg
     2101 ctgccgtaaa atactttcgt gacgcttgcg ggacaaaacg cctggcacat cgcaaccggc
     2161 gtcgcgttgc cgcgcgccag cagcggaaaa tacttgccat cgcagcttcg cacgcaaaat
     2221 gccggcccgg agccgcccga tacgaccggg cgcggcgggg gtgctgcctg ctggttgagg
     2281 ccgaacggat cggcgaagaa attcgcctgc ggtggggcct tttgctgctg ttgcttcggg
     2341 ctgccgccaa acagaaaatc gaacaggccc tcggcggatg cgatgcgcgg cgccagcgca
     2401 acagacgctg tgagtgccgc agtggcgata agtatcgcgc ggcgccgcat gggttgataa
     2461 ggcaactctt tacgcaacgc ttactccacc ccacgcaacg gaacccgctg tgccgagaag
     2521 tatctcagca ccgcttcaca ttagtgcggg atggtaaacg aggtatgagc gagcgccgaa
     2581 aaagccgaaa agaaattcgg attgtttgtc atcgcttcac gacgaagtgc cggccgcgtg
     2641 ccccggatgc tgcgcagcgc catcagcgcg ttcacgcgcg tcttcgacgc gctatgcggt
     2701 gcgctgctga tccggggccc atgccgcaca atgtaaactg tgggtcccgg acgcggagca
     2761 gcgtgaaaaa cgctgcaccg cgtccgggac acgagtctcg gcctttcact caggcgggcg
     2821 aggaagaaga caagaaatca acccttcagg aattcgccgg ccttgtagag cgagcggaac
     2881 ggaaggccgg cctgcgcgaa ggtttcggcg gcgccttcgt cgcgatccac catggtgaac
     2941 accagtgcga cctcgccgcc ggcatcgcgc accgcctcga ccgccttcag cgccgagccg
     3001 ccggtcgtgg tgacgtcctc gacgatcacc acgcgcttgc cttgcaggct ctcgcccttc
     3061 gccagccctt cgaccgcgag ccgggcgcca tgttccttcg gcttcttgcg gacaaagaat
     3121 gccgcgatcg gatggccctt gatccaggac aactgcgcga tcgcgccggc gagcggcacc
     3181 gcgcccattt ccagtccgcc gacataatcg aggttgtcat ccttgagtgc ctcgtatgtc
     3241 agctccgcga gcaatgaggc gccttcggga tcgagcatgg tcggcttgag attgaagtag
     3301 aaatcgcttt tgcggcccga agccagcgtg atttcgccac ggccgaacga gcgcttgcga
     3361 atgatctcgg cgaggcgggc gcgggaggct gacttggaca aggcgttgca ctcgggggct
     3421 ggagcgggct gcaatctatc ggcggatccc tgcacattcc agagccgatg tggcgccgtc
     3481 aacagcgtgg aggcctaatc atccgcgtgc gtgggcggtc ccctattccg cgacggcggt
     3541 tgtaaggccg tgaggttccg gggtactgga taccccgctt tcgtgagggt atgacaaaaa
     3601 tgacgatcga actgcacact tggaacacgc cgaacggccg caagatcagc gtcgcgctgg
     3661 aggaaatggg gctgccgtac aaggttttcc cgatcaatat caccaagggt gagcagatgg
     3721 cgccggcgtt tctggcgatc agcccgaaca acaagatccc cgcgatcgtc gatccggacg
     3781 gtcccgatgg caagcgcgtc agcgtgttcg agtccggcgc gatcctgctc tatctcggcg
     3841 agaagaccgg caaattcctg cccaaaccgc tctcggagcg catcccggta
//
LOCUS       DASWGM010000387         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2117209,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000387 DASWGM010000000
VERSION     DASWGM010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117209"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VGA15_11220"
     CDS             <1..106
                     /locus_tag="VGA15_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX9588298.1"
                     /translation="QEELWPKFGYEGSSAEHGGYIKRGFNDIDWLPKV"
     gene            146..1714
                     /locus_tag="VGA15_11225"
     CDS             146..1714
                     /locus_tag="VGA15_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9588299.1"
                     /translation="MAKFDLNDDGVVVIVGSGAGGGTLGNELAQKGVKVVILEAGPRV
                     ELQDFINNEWESFTQLAWTDMRTTSGNWRVAKDFANLPAWIVKSVGGSTVHWAGASLR
                     FDEHEFRIKSAYGSVPGANLLDWPITLAEMEPWYAKAEDKMGVTRTNNIPGLPGNNNF
                     KVMEAGAKKLGYKEVHTGRMAINSEPRDGRGSCQQIGFCFQGCKTGAKWSTLYAEIPK
                     GEATGNLEVRPNSMALKIEHDASGKATGVVYADEAGKTQRQKARIVAVAGNSIESPRL
                     LLNSASSMFPDGLANSSGQVGRNYMRHMTGSVYAVFDKSVHMYRGTTMAGIIHDEARH
                     DPSRGFMGGYEMETLSLGLPFMAAFLNPGAWGRSFTSAMEGYPRMAGMWLVGEDMPQE
                     TNRITLDPKAKDKFGLPVASVHYDDHPNDVAMRDHAYKQGSAVYEAIGATVTYPTTPY
                     PSTHNMGTNRMSAKAKDGVVNKFGQTHDIKNLFVSDGSQFTSGAACNPTLTIVALAVR
                     QADHIAGAMQRKEI"
     gene            complement(1785..2093)
                     /locus_tag="VGA15_11230"
     CDS             complement(1785..2093)
                     /locus_tag="VGA15_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX9588300.1"
                     /translation="MCQLFAHQPQRDYESQTRSLRIGGHCTSIRLEMSFWDTLEEIAG
                     KEGLSLPKFLTKLHTEVLDHHGEVNNFASLLRCSCLIYRSKNAVVTPDFRAPVLDAAE
                     "
BASE COUNT          531 a          760 c          762 g          427 t
ORIGIN      
        1 ccaggaagag ctgtggccga agttcggcta cgagggctcc tccgccgagc atggcggcta
       61 catcaagcgc ggtttcaacg acatcgactg gctgccgaag gtctgaggac aacttcgcgc
      121 agaacatcag ttgggaggat acacaatggc aaaattcgat ctgaatgacg atggtgtggt
      181 ggttatcgtt ggctccggcg ccggcggcgg aacgcttggc aatgaactcg cgcagaaggg
      241 cgtcaaggtg gtgatcctgg aggccgggcc gcgcgtcgag ctgcaggatt tcatcaacaa
      301 cgagtgggaa agtttcacgc agctggcgtg gacggacatg cggacgacgt cgggcaactg
      361 gcgcgtggcg aaggatttcg ccaatctgcc ggcatggatc gtcaaatcag tcggcggatc
      421 gaccgtgcat tgggccggcg cgtcgttgcg gttcgacgag catgaattca gaatcaagag
      481 cgcctacggc agcgtgccgg gcgccaatct cttggactgg ccgatcacgc tggctgaaat
      541 ggagccgtgg tacgccaagg ccgaagacaa gatgggcgtc acccgcacca acaacattcc
      601 gggattgcca ggcaacaaca acttcaaggt gatggaagcc ggcgccaaga agctcggcta
      661 caaggaggtc cacaccggcc ggatggcgat caacagcgaa ccgcgcgacg gccgcggctc
      721 ctgccagcag atcggcttct gcttccaggg ctgcaagacc ggcgcaaaat ggtcgacgct
      781 ttatgcggag atcccgaagg gcgaagccac cggcaatctc gaggttcgtc cgaacagcat
      841 ggcgctcaag atcgaacacg acgcatccgg caaggcgacc ggcgtcgtct acgccgacga
      901 ggctggaaag acccagcgtc agaaggcgcg catcgttgcc gtcgccggca attcgatcga
      961 aagcccgcgt ttgctgctca acagcgcctc cagcatgttc cccgatggcc ttgccaacag
     1021 ctccggccag gtcggccgca actatatgcg tcacatgacc ggcagcgtct atgcggtgtt
     1081 cgacaagtcg gtccacatgt atcgcggcac gacgatggcc ggcatcatcc acgacgaggc
     1141 gcggcacgat ccgtcgcgcg gcttcatggg cggctatgag atggaaacgt tgtcgcttgg
     1201 cctgcccttc atggccgcgt tcctcaatcc gggcgcctgg gggcgctcct tcacgtcggc
     1261 gatggaaggc tatccgcgca tggccggcat gtggctggtc ggcgaggaca tgccgcagga
     1321 aaccaaccgg atcacgctcg atccgaaagc caaggacaag ttcggcctgc cggtggcgag
     1381 cgtgcattac gacgaccatc ccaacgatgt cgcgatgcgc gaccacgcct acaagcaagg
     1441 ctccgccgtc tatgaggcga tcggcgcgac cgtgacctat cccaccacgc cctatccctc
     1501 aacgcacaac atgggcacca accgcatgag cgcgaaagca aaagacggcg tggtgaacaa
     1561 gttcggccag acccacgata tcaagaatct gtttgtgtct gacggcagcc agttcaccag
     1621 cggtgccgcc tgcaatccga cccttaccat cgtggcactg gcggtccgcc aggcggacca
     1681 catcgccggc gccatgcaac gaaaggaaat ttaatccatc tgatatgaaa aggctccgtc
     1741 cgcgacacgg gtggagcctt tatcctgccg cccatcgaga acgactattc cgcggcgtcg
     1801 aggacaggcg cgcggaagtc gggcgtcacc accgcatttt tcgagcgata gatcaggcag
     1861 gaacaacgca gcagcgacgc gaaattattg acctcgccgt gatgatcgag cacttcggtg
     1921 tgaagcttgg tcaggaattt cggaaggctc aggccttcct tgccggcgat ctcctccagc
     1981 gtatcccaga acgacatttc cagccggatc gacgtgcaat gccccccgat ccggagcgat
     2041 cgggtctgcg attcatagtc gcgctgaggc tggtgtgcga acagctggca catgggtatc
     2101 ctcccgatct cttttaaaac ttttctaaac gatatacccg gtcggcgcgg gtcacaatgt
     2161 tctcgtcatg gcagctcaag ccgaggcgcg gatcgggtaa atgtggcggt tgaggcttgg
     2221 ttcgccatga acagcgggga gagctgccaa aacccctcgg gtttatcagc cgccgccctc
     2281 ccgccggacg gtaaaagctc ctaaattgct tccccactcc acgagtcgcc gcccatgccc
     2341 gtccgccagc tccccgaaca ggtcgtcaac cgcatcgccg ccggcgaagt ggtcgagcgc
     2401 cccgcgagcg tggtcaagga actggtggaa aacgccatcg atgccggcgc cagccggatc
     2461 gacattttta ccgatggcgg
//
LOCUS       DASWGM010000388        14065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1273574,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000388 DASWGM010000000
VERSION     DASWGM010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14065
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273574"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VGA15_11235"
     CDS             <1..525
                     /locus_tag="VGA15_11235"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX9588301.1"
                     /translation="DAVQAMPKLGFFPGSTLGNFEPHEACAFMRSARDILGEGAQMVI
                     GVDLEKNERVLYDAYNDAAGVTARFNLNVLVRINRELGGNFDTAGFTHRAIYNRDRHR
                     IEMHLISRKTQTVRVLGRSFSFRAGESIHTESSYKYSLERFAALARGSGWSPRHSWTD
                     KAGMFSVHALVASD"
     gene            complement(501..1736)
                     /locus_tag="VGA15_11240"
     CDS             complement(501..1736)
                     /locus_tag="VGA15_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX9588302.1"
                     /translation="MAGDNPRGALFRLSVPNQHSRVTYAELFFDLVFVFAVTQISHTL
                     LGRFTPLGAVQTTLLFLAVWWVWVYTSWITNWLDPETTPVRVLLFLLMLGGLVLSTSI
                     PKAFESRGLWFALAYAAMQVGKTVFLWVSTPSSRTLARMNAVRITAWLSISAIFWIAG
                     GLAEGHSRLLLWLAALGIEYISPAVRFWIPKYGASSIEDWVVEGGHMAERCAGFIIIA
                     LGESIVVTGATFADLAWTTETVVAFASAFIGSLAMWWIYFHKGVEAGSELISKSSEPG
                     RLARLAYTYLHMPIVAGIILSAVADELVLTHPADHSDSRTVLSAIGGPLLFLLGTILF
                     KHTIRGFLQLSHGVGIVALGGLAWFASELSPLMLSIVTTAIMIIVAVWESISLKSGPA
                     VSRTEGSPTAESQSEATSA"
     gene            complement(1760..1996)
                     /locus_tag="VGA15_11245"
     CDS             complement(1760..1996)
                     /locus_tag="VGA15_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX9588303.1"
                     /translation="MPMDAHDIESMIKAAIPDAEVTIRDLAGDGDHYAATVISESFRG
                     KSRVQQHQIVYQSLRGQMGGVLHALALQTGVPEG"
     gene            2169..2528
                     /locus_tag="VGA15_11250"
     CDS             2169..2528
                     /locus_tag="VGA15_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX9588304.1"
                     /translation="MKLPGPDHPITITANPKRIRVSADGVVIADTTHALTLKEATYPA
                     VQYVPREDAKMALLARTDRVTHCPYKGDANYFSIMADGKTIENSIWTYETPFPAMAEI
                     SGHLAFYPDKVKIEEVG"
     gene            complement(2628..2801)
                     /locus_tag="VGA15_11255"
     CDS             complement(2628..2801)
                     /locus_tag="VGA15_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX9588305.1"
                     /translation="MTILKWALIFFLVSIVAGILGFTGVSAASADIARFLFYVFVVIF
                     LVLLILGLTIFRV"
     gene            3023..4207
                     /locus_tag="VGA15_11260"
     CDS             3023..4207
                     /locus_tag="VGA15_11260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9588306.1"
                     /translation="MTSISSSVAPAETRATPRTSARNAALDRARTFLTLVVLVHHAVI
                     PYTYFGHTDPKSWLGFDAIVLATDSFFMAMFFFLSGLFVWPSLAHKAPRIFFRERLLR
                     LGLPFAIAAFTVIPIAYYAIALRQQPDLGFSAFWWKTITVGPWPSGPVWFVWVLLAFD
                     LTASLLYRISPTLLDPINRLSLRGFGRPADFFLFLLAVTAVVYIPARVYFGAGRWFSF
                     GPFDVQASRVLLYAAYFYIGAGIGAANFDRGLLSADGRLAKSGWGWVVATLVPYCLLW
                     VLITIKREILGNPVILPDWYEAAYGLFFVAFSAAILFAILAFFLRFKRSGWSVLDPMQ
                     GDAYGMFLVHYPIVLWLQYWLFDYDLPAIVKALVAFVLTVALSWAVTAALRKIPGATK
                     VL"
     gene            4277..4813
                     /locus_tag="VGA15_11265"
     CDS             4277..4813
                     /locus_tag="VGA15_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9588307.1"
                     /translation="MVRYRRNLVPGGTFFFTVTLADRRSSLLVDHVGLLRAAFRHARD
                     RKSFAIDAIVILPDHLHAILTLPSGDSDFSGRWKAIKATFTRSIVATGAAIVRDDRGE
                     YALWQRRFWEHTIRDDQDFERCVNYVHFNPVKHRLVSTPGEWPFSSLHRYVRAGILPH
                     DWGGDDRAGNTNFGERTG"
     gene            complement(4853..7078)
                     /gene="purL"
                     /locus_tag="VGA15_11270"
     CDS             complement(4853..7078)
                     /gene="purL"
                     /locus_tag="VGA15_11270"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX9588308.1"
                     /translation="MNQPQITPELVASHGLKPDEYERILKLIGRVPSFTELGIFSAMW
                     NEHCSYKSSRLHLKGLPTKAPWVIQGPGENAGVIDIGDGQAVVFKMESHNHPSYIEPY
                     QGATTGVGGILRDVFTMGARPIACLNALSFGAPEHPKTRHLVSGVVAGVGGYGNSFGV
                     PTVGGQVRFHTRYDGNILVNAMAVGLADSDKIFYAAASGVNMPIVYLGSKTGRDGIHG
                     ASMASAEFDDDSAEKRPTVQVGDPFAEKLLLEACLEIMQADCVIAIQDMGAAGLTCSA
                     VEMGAKGDLGVDLDLDAVPTRETGMSAYEMMLSESQERMLMVLKPEKEKQAEAIFRKW
                     GLDFAVVGYTTPSKRFVVKHGGDVMADLPIKELGDEAPLYDRPHVASPSLPVVHARDV
                     KPPTGISAALETLIATPELCSKRWVWEQYDHVILGNTVQRPGGDAAVVRVQDGPKGLA
                     MTVDVTPRYCEADPFEGGKQAVAEAWRNITAVGGRPLAITDNLNFGNPERPEIMGQFV
                     GCLKGISEACRALDFPVVSGNVSLYNETRFPGEELGRGILPTPSIGGVGLLDDFTKSA
                     TLAFKAEGEAILLIGETHGWLGQSVYLRDICGREEGAPPPVDLAAEKRNGDVVRGMIH
                     AGTATSAHDVSDGGLLIALAEMALAGGIGAQLLAAPGEIVPHAYWFGEDQARYIVTVP
                     AEQAGLVLAKMKGAGVPCARIGTTGGDTIAIVGEPEVSVKSLKTGFEGWFPAYMNGKA
                     S"
     gene            7260..7751
                     /locus_tag="VGA15_11275"
     CDS             7260..7751
                     /locus_tag="VGA15_11275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9588309.1"
                     /translation="MDDLTKSAFLNRPDLHVETEGEFKGWRTWSRDNFESHNGPFWHR
                     MEDDGQIRCAFRVEKKHLNGMSNVHGGAFMAFADYCLFAMAAPVLQGPGVTVSFGCEF
                     LDAVREGELVEGTGDIMRAGGSMIFLRGVLKSAERPLFTFSGTIKRVKRRMPAVPGQE
                     SKS"
     gene            9061..9339
                     /locus_tag="VGA15_11280"
     CDS             9061..9339
                     /locus_tag="VGA15_11280"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9588310.1"
                     /translation="MIRSFRSKALRRYAETGDGSKLSVQNPDRIRLILARLDAAIRPQ
                     DMNLPGLRFHGLKGRGKGRFAVDASGHWRITFAWDGEDAIDVDLEDYH"
     gene            9351..9659
                     /locus_tag="VGA15_11285"
     CDS             9351..9659
                     /locus_tag="VGA15_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX9588311.1"
                     /translation="MSGLKPTHPGELLREIVFPALKMPKREIADLLGISRQMLHGILA
                     ERHPITPTMALRIGKLLGTSPESWLRMQEAYDLRKAEQTMRKELKAIPTLRTEATEQA
                     "
     gene            9685..9963
                     /locus_tag="VGA15_11290"
                     /pseudo
     CDS             9685..9963
                     /locus_tag="VGA15_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
     gene            complement(9955..10692)
                     /locus_tag="VGA15_11295"
     CDS             complement(9955..10692)
                     /locus_tag="VGA15_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9588312.1"
                     /translation="MPFALVILLLDITLVYHASRTGRLQPWAFIILMIPGIGALAYIL
                     VELVPEMLSGPGAQQARRRVANRLDPEKLYRELSDRLAVTDTIANRVALAAECLRIER
                     FGEAERHYDHILQLPLGDDPVYALGKAKAEFGRNRPAEALATLDDLQKRWPDFQSAEG
                     HLLYARALGAVGRVDEALEEFHAVAAYFPGAEARVRYGLLLNLVGRTAEARVVFNELL
                     IQMRRAPKYLREAQTEWLSIAEKQLSA"
     gene            complement(10834..12438)
                     /locus_tag="VGA15_11300"
     CDS             complement(10834..12438)
                     /locus_tag="VGA15_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588313.1"
                     /translation="MLIHKATCTVALAAVLLSHQPAMAETTLRIAMTASDIPTATGLP
                     NNGFEGMRFLGYPIFEGLVLWDLTRTDRLAALRPGLAEKWEQDPEDNKTWIFHLRHGV
                     KFHDGTDFNADAVIWNLDRYFNSTSPQFEPPSSAMSRARVPVMGSYKKIDDATVAITT
                     TKPASYFPYMAVYLLFTSPASFEKAGGDWAKVATLPAAGTGPFRITKIVPRQEADLAR
                     WDKYWDPAKMAKVDNVVLMPIPEANSRLAALRSGQVDWIEVPPADGIASLKSAGFAIT
                     TGSYPHVWPWFYNIGAANSPFKDVKVRQALNYCIDREGLVSLLNGTAEPSAGWLKTGD
                     PDFGNPVNRYKFDPAKGKALLAEAGYTPAKPLSFKALISNSGSGQMLPLPMNEFLQQN
                     LKEACGVTVEFDVIEWQVLLTAARATPDSPNLRGAMALNISSPSSDAGVMARYFLAAN
                     FSPNGFNFEQWRDDEFEDALKTISEATDPKVMSTAYRKAHERLVDNPPWLYIVHDLNP
                     RAMSPKVKGFVSPQSWFVDLTLVSVQ"
     gene            complement(12671..13372)
                     /gene="purQ"
                     /locus_tag="VGA15_11305"
     CDS             complement(12671..13372)
                     /gene="purQ"
                     /locus_tag="VGA15_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX9588314.1"
                     /translation="MKSAVLVFPGINRERDMARALKLISGHEPQMVWHAETALPAGID
                     LVVVPGGFSYGDYLRCGAIAARAPIMDAVRAFAAQGGLVLGVCNGFQILCEAGLLPGV
                     LMRNARLKFICHDVHLRVERSDTPFTRGYNAGQVIRVPVAHGEGNYEADEETLKRLEG
                     EGRVLYRYCSAEGVVDEKSNINGAAQSIAGIVNERGNVLGMMPHPENHVEDIMGCTDG
                     RGLFAGLMAHLERAA"
     gene            complement(13386..13628)
                     /gene="purS"
                     /locus_tag="VGA15_11310"
     CDS             complement(13386..13628)
                     /gene="purS"
                     /locus_tag="VGA15_11310"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX9588315.1"
                     /translation="MKARVTVTLKSGILDPQGKAIEGALKSLGVDGVASVRQGKVFDI
                     ELSGSDAAEAEAALKDAADKLLANTVIENYRVEVLS"
     gene            complement(13637..>14065)
                     /locus_tag="VGA15_11315"
     CDS             complement(13637..>14065)
                     /locus_tag="VGA15_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9588316.1"
                     /translation="PRSIIEFYYKNDQLNDPMVSEEHITAFGWATPQEIDDIMALAIR
                     VNDFLTGLFLGIGIRLVDFKMECGRLFENEMMRIIVADEISPDSCRLWDVKSNEKLDK
                     DRFRRDLGGLLEAYTEVAKRLGILMENERPIGSGPVLVKS"
BASE COUNT         2693 a         4415 c         4227 g         2730 t
ORIGIN      
        1 gacgcggtgc aggcgatgcc gaagctcgga tttttcccgg gatcgacgct cggaaatttc
       61 gagccgcacg aagcctgcgc cttcatgcgc agcgcccgcg acatccttgg tgagggcgcg
      121 cagatggtga tcggcgtcga cctcgaaaag aacgagcgtg tgctgtatga tgcctacaac
      181 gacgccgccg gcgtcaccgc acgcttcaat ctcaacgtgc tggtccgcat caatcgcgag
      241 ctcggcggca atttcgacac tgccggattt acccatcgcg cgatctacaa tcgcgaccgc
      301 caccgcatcg agatgcacct gatcagccgc aagacgcaga ccgtgcgcgt gttggggcgg
      361 agtttttcat tccgcgccgg cgaaagcatc cacaccgaga gcagctataa atacagtctc
      421 gagcgttttg cggcgctggc gcgcggctcg ggctggagcc cgcgccactc gtggaccgac
      481 aaggccggca tgttttcggt tcatgcgctg gtggcctcgg actgagactc cgcggtcgga
      541 gacccttcag tcctggatac ggccgggcct gatttcagcg agatcgattc ccacacggcg
      601 acaatgatca tgatcgcggt ggtgacgatc gacagcatca acggcgatag ctcgctggcg
      661 aaccaggcca gcccgccaag ggccacgatg ccgacgccat gcgacagctg gagaaagccg
      721 cgaatggtgt gtttgaagag gatggttccg agcagaaaca gcagcggtcc gccgatcgca
      781 ctgagcaccg tcctcgaatc ggaatgatcc gccggatgcg tcagcacgag ctcatcggcg
      841 accgccgaaa gaatgatgcc ggccacgatc ggcatgtgca gataggtgta ggccagcctc
      901 gccagccgcc cgggctcgct ggattttgaa atcagttcgg accccgcttc gacgcccttg
      961 tggaaataga tccaccacat tgcgaggctg ccgataaagg ccgacgcaaa ggcaaccacg
     1021 gtctccgtgg tccaggccag atcggcgaac gtcgcgcctg tcaccacaat ggattcgccg
     1081 agcgcgatga tgatgaatcc ggcgcagcgc tccgccatgt ggccgccttc aaccacccag
     1141 tcctcgatcg aagacgcgcc gtatttcggg atccagaacc ggaccgccgg cgagatatat
     1201 tcgatgccca gcgccgccag ccaaagcagc agccgcgagt gcccttccgc aaggccgccc
     1261 gcgatccaga agatggccga tatcgacagc cacgccgtga tccggaccgc attcatgcgc
     1321 gccaaagttc gggaagacgg tgtcgacacc cacagaaaga ccgtcttgcc gacctgcatc
     1381 gcggcgtagg ccagcgcgaa ccagagcccc ctcgattcaa aggcctttgg aatcgaggtc
     1441 gagagcacca gcccgccgag catcagcaga aacagcagca cccggaccgg cgtcgtctcg
     1501 ggatcgagcc agttggtaat ccaggaggtg tagacccaca cccaccagac agccaggaac
     1561 agcagcgtcg tctgcaccgc gccgagcggg gtgaaccggc cgagcagggt gtgcgatatc
     1621 tgcgtgaccg cgaaaacgaa caccagatcg aaaaacagct cggcataggt gacgcggctg
     1681 tgctggttgg gcacggagag acgaaacagc gcgccgcgcg gattgtcccc agccatggac
     1741 tacccctgcc ccgcgccgat tacccctcag gcaccccggt ctgcaacgcc agcgcgtgaa
     1801 gcacgccgcc catctggccg cgcagcgatt gatagacgat ctgatgctgc tggacgcggg
     1861 atttgccgcg gaacgactcc gaaatcacgg tcgcggcata gtggtcgcca tcgccggcca
     1921 gatcgcggat cgtcacctcg gcgtcgggga tcgccgcctt gatcatcgat tcgatatcgt
     1981 gggcgtccat cggcatccag gttttctcca atccggcatt tcggcgagcg gccgacaccg
     2041 gccgcaggaa cttagcgcgg acgcccttct aggtcacgct ccgttgcact atattcccga
     2101 tccaatcctt gcaaaaccgc tggatcgcat cttgtgatca caacgggctg accataaggc
     2161 gtacaaccat gaaactccca ggtcccgacc acccgatcac cattacggcc aacccgaagc
     2221 gcatccgtgt ctccgccgat ggcgtcgtga tcgccgacac cacccatgcc ctgacgctga
     2281 aggaagcgac ctaccccgca gtgcagtatg tgccgcgaga ggatgccaaa atggcgctgc
     2341 tggcgcgcac cgaccgggtg acccactgcc cctataaggg ggatgcgaat tatttcagca
     2401 tcatggccga cggcaagacc attgaaaatt cgatctggac ctatgaaacg ccgttcccgg
     2461 cgatggcgga aatctccggc catctggcgt tttacccgga caaggtgaag atcgaggagg
     2521 tgggctgagg gtcagaggat cgtagcccgg acgagcgaaa gcgaatccgg ggtcggggtc
     2581 acgttcgttc ccggatttcg cttcgctcat ccggggctac acccgcttta caccctgaag
     2641 atcgtcaggc cgaggatcaa caggaccagg aagatcacga cgaaaacgta gaacaggaag
     2701 cgggcgatat cggctgatgc ggccgatacg ccggtgaagc cgaggatacc ggcgacgatc
     2761 gacaccaaaa agaagatcag cgcccatttc agaattgtca tcgcccatta ctccagcctg
     2821 ctggccgccg gatccgcagc cctttacaaa aatcgctgat ctaacttcga gcgatggcac
     2881 tggttccaca acgatgaggc gggcggcgtt gagatcgccg ccccgcccgg caggccactg
     2941 gttgcgtcgc gctataattg ggccttgctg aatccggctc gcggcggcaa catctgaagc
     3001 gaccaccctg ccgggggcgc gcatgacgtc aatttccagc tcagtggctc ctgcggagac
     3061 cagggcaaca ccccgcacct ccgcgcgcaa cgcggcgctc gatcgcgccc gaaccttcct
     3121 gaccctggtg gtgctcgtcc accacgccgt cattccctat acttacttcg gtcataccga
     3181 tcccaagtcg tggctcggct tcgacgcgat cgtgctcgcc actgacagct tcttcatggc
     3241 gatgttcttt ttcctctccg gtctgttcgt atggccaagc cttgcccaca aggcgccgcg
     3301 gatcttcttt cgcgaacgct tgctgcggct cggcctgccc ttcgcgatcg ccgcgttcac
     3361 cgtcatcccg atagcctatt acgccatcgc gctgcgccag cagcccgatc tcggcttttc
     3421 ggcattctgg tggaagacga tcaccgtcgg cccctggccc agcggcccgg tatggttcgt
     3481 ctgggtgctt ttggctttcg accttacggc gagcctgctg taccggatct cgcccacgct
     3541 gctcgatccg atcaatcgcc tgtcgctgcg cggcttcggc cggccggccg acttcttcct
     3601 gtttcttctg gcggtcaccg ccgtcgtcta tattccggcg cgggtctatt tcggagcggg
     3661 ccgctggttc agtttcggac cgttcgacgt gcaggccagt cgcgtgctgc tgtatgcggc
     3721 ctacttctac atcggagccg ggattggcgc ggcaaatttc gatcgcggcc tgctgagcgc
     3781 ggacgggcgg ctggcgaaga gcggctgggg ctgggtggtc gcgacgctcg ttccgtattg
     3841 cctgttgtgg gtgctgatca ccatcaagcg cgagatattg gggaatcccg tgatcttgcc
     3901 ggattggtat gaggctgctt acggcttgtt cttcgtcgcc ttcagtgcgg ccatcctgtt
     3961 cgcgatcctg gcatttttct taaggttcaa acgttcgggc tggagcgtgc tcgatccgat
     4021 gcagggcgac gcctacggta tgtttctggt gcactacccg atcgtgctct ggctgcaata
     4081 ctggctgttc gattacgacc tgcccgccat cgtcaaggct ttggtcgcat tcgtcctgac
     4141 cgtggcgttg agctgggctg tgacggcggc gctgcgcaag attccgggtg cgacaaaggt
     4201 gctgtgagcg acgccgttca tataagcgca ttgtagcccg gatgcagcga agcgaaatcc
     4261 gggataggct tctcggatgg tccgataccg ccgcaatctc gtccctggcg ggacgttctt
     4321 tttcacggtg acgctggcgg accggcgatc atccctgctc gtcgatcatg ttggattgct
     4381 gcgcgccgcg tttcgccatg cgcgggatcg caaatctttt gcgatcgatg cgatcgtaat
     4441 tttgccggat catctgcacg ccattctgac tttgccgtcc ggtgactccg atttttccgg
     4501 ccgttggaag gcgatcaagg ctacgttcac ccggagtatt gtcgcaacgg gcgcggccat
     4561 tgtccgggat gaccgtggcg agtatgcgct ttggcagaga cgtttctggg agcacacgat
     4621 acgcgacgat caggatttcg aacgatgcgt gaactatgtt catttcaacc cggtcaagca
     4681 tcgattggta tcgacaccgg gtgaatggcc gttctcgtca ctgcatcgat acgtccgggc
     4741 cggcatattg ccgcacgatt ggggtggaga cgaccgtgcg ggcaatacca attttggcga
     4801 gcgcactgga tgatcccgga ttgcgcttcg cttcatccgg gctacgctct tttcaggacg
     4861 ccttgccgtt catgtaggcc ggaaaccagc cctcgaagcc ggtcttgagc gacttcaccg
     4921 aaacttccgg ctcgccgaca atcgcgatcg tgtcgccgcc ggtggtgccg atccgggcgc
     4981 aggggacgcc ggcgcccttc atcttcgcca gcaccagccc ggcctgctcc gcaggcacgg
     5041 tcacgatgta gcgcgcctga tcctcgccaa accaatacgc gtgcggcacg atctctccgg
     5101 gcgccgccaa gagctgcgcg ccgatcccgc ccgccaacgc catctccgcc agcgcgatca
     5161 agagaccgcc gtcggaaacg tcgtgcgccg atgtcgcggt gcccgcatgg atcatgccgc
     5221 gcaccacatc gccgttgcgc ttttccgccg cgagatcgac tggcggcggc gcgccctctt
     5281 cgcggccgca gatgtcgcgc aggtaaaccg actggccgag ccagccatgg gtttcgccga
     5341 tcaagaggat cgcctcgccc tcggccttga acgcaagcgt tgccgacttg gtaaagtcat
     5401 cgagcagccc gacgccgccg atcgacggcg tcggcagaat cccacggccc aattcttctc
     5461 caggaaatcg ggtctcgttg tagagcgaga cattgccgga cacgaccggg aagtcgagcg
     5521 cgcggcaggc ttccgagata cctttcaagc agccgacgaa ctggcccatg atctccggcc
     5581 gttccggatt gccgaaattg aggttgtcgg tgatcgccag cggccggccg ccgacggctg
     5641 tgatattgcg ccaggcctcg gccaccgcct gcttgccgcc ttcgaacggg tcggcctcgc
     5701 agtagcgcgg ggtgacgtcg accgtcattg ccagaccctt cggtccgtcc tgaacccgca
     5761 ccacggcggc gtcgccgccg ggacgctgca cggtgttgcc cagaatcaca tgatcgtatt
     5821 gctcccacac ccagcgtttc gagcaaagct ccggcgtggc gatcagtgtt tccagcgccg
     5881 ccgaaattcc cgtcggtggc ttcacgtcgc gcgcatggac caccggcaac gacggcgatg
     5941 ccacatgcgg ccggtcgtag agcggcgctt cgtcgcccag ttccttgatc ggcagatcgg
     6001 ccatcacgtc gccgccatgc ttgacgacaa agcgcttgct cggcgtggtg tagccgacca
     6061 cggcgaaatc cagcccccat ttgcgaaaga tcgcttcggc ctgtttttcc ttctcgggct
     6121 tgagcaccat cagcatgcgc tcctggctct ccgacagcat catctcgtag gcgctcatgc
     6181 cggtttcgcg ggtcggcacc gcatcgagat cgagatcgac gccgagatcg cccttggcgc
     6241 ccatctccac cgccgagcag gtcaggccag ccgcgcccat atcctggatc gcgatcacgc
     6301 aatcggcttg catgatctca aggcaggctt ccagcagcag cttttccgcg aagggatcgc
     6361 cgacctgcac ggtggggcgc ttctcggccg agtcgtcgtc gaactcggcc gacgccatcg
     6421 aggcgccgtg aatgccgtcg cggccggtct tggagcccag atagacgatc ggcatgttga
     6481 cgccggaggc ggccgcataa aagatcttgt cgctgtcggc gaggccgacc gccattgcat
     6541 tgaccaggat gttgccgtca tagcgggtgt ggaaacgcac ctgcccgccg acggtcggca
     6601 ccccgaacga attgccatag ccgccgacgc cggcgacgac gcccgacacc aggtgccgcg
     6661 tcttgggatg ctccggcgca ccaaaactca gcgcattgag gcacgcgatc gggcgcgcac
     6721 ccatggtgaa aacatcgcgc aaaatgccgc cgacgccggt ggtcgcgccc tgatagggct
     6781 cgatgtagct cggatggttg tggctctcca tcttgaacac gaccgcctgg ccgtcgccga
     6841 tgtcgatgac gccggcgttc tcgcccggcc cctggatcac ccacggcgcc ttggtcggca
     6901 accccttgag atgcagccgc gaggatttgt acgagcaatg ctcgttccac atcgccgaga
     6961 aaatccccag ctcggtgaaa ctcggcaccc ggccgatcag cttgagaatg cgctcatatt
     7021 cgtcgggctt caggccatgg ctggcgacga gttcgggggt gatttgcggt tggttcatga
     7081 ggcttcttaa agcgattgag gtcccttatt agggagatcg ggaggggccg aaaaggcctt
     7141 ttatggcgca atttcctgct ttccatagat ttgcggacgc ccaatgcata acgggggttt
     7201 gcacatcggg cgtggatcgg atttaagggc tcaaagacct caaatgaagg ccaaatcgct
     7261 tggacgacct caccaaatcc gcattcctca accgccccga tctgcatgtc gaaaccgagg
     7321 gcgaattcaa gggctggcgg acctggagcc gggacaactt cgaatcccat aacgggccgt
     7381 tctggcaccg gatggaggat gacggccaga tccgctgcgc gttcagggtc gagaagaaac
     7441 acctcaacgg catgagcaat gtccatggcg gcgccttcat ggcattcgcg gactattgcc
     7501 tgttcgcgat ggccgcgccg gtgttgcagg ggccgggggt gacggtgtca ttcggctgcg
     7561 aatttctcga cgccgtgcgc gagggcgaac tggtcgaagg caccggcgac atcatgcgcg
     7621 ccggcggctc catgatcttt ttgcgcggcg ttttgaaatc ggcggagcgt ccgctgttca
     7681 cgttttccgg caccatcaaa cgcgtgaagc ggcgaatgcc ggcggtgcca ggccaagaga
     7741 gcaaatcctg atctgcatcg accttacccc cgatcatctc ccgccagggg gaaagggaat
     7801 tcactgccat ggccgcggta ctgagctttc tcttcaacgg cggtcccttc aatttgctaa
     7861 cagcaaacgt aacccggacg caggttcgct ccccgcggcg cactggcgcc cgagctgtta
     7921 aaatctcatc cgcccctgag acagagggcg tggggaatgc cgggtgcccg atgcacccgc
     7981 agcctcgtgt gcgctatggt agttgagatt gcacacgagt attcacagcg gtcgcaccga
     8041 aaatcacccg gcatccccgc acgcaatggt tttaacggtt tatttcgcgc tctcctcggc
     8101 gatcggctct tgatgccgtc gcctgcggat taaaggtttt gccggacccg gttgagccca
     8161 acgtcacctc cgcagacttg acgctcgcat tgaggcgtca ggaccacacg acttcaccgt
     8221 ccgcgggcaa catcgtcgtc ttgcgtgctg tggaccgctc acggagagca atccgccctg
     8281 ccatcccgtc acacgcctaa cgctgccgcg tccaccgcat ctcatcccgc gtgtctgacg
     8341 atcgcgatac gcccctcgtg tgggacaaga cggcacgctt atagaggtga tttggatcgg
     8401 cgtggaagcg gaatattttc gcgaggggga ctggactacc caaatcagct tgaattcgtt
     8461 caacaaattc gcgccagcgt gaattgggca aaagccgaaa cttcatccgt catcgcaacg
     8521 gatgcgaaga tatttgaccc gtcgggcaaa tcaccggcat atcttcatcg tcacagtcga
     8581 aggccgacgg gcaaatcaca gtcggtgttg ccaacaacgc ttctcgcatc aagccactcc
     8641 cttgcatcaa gcagacagac atcgcccggc ttgatcgggc gactcagaat tgcggaagtc
     8701 agggtgacga cgactgaggt tgcggctgca aatagtgata tcggcggccc agaagcccaa
     8761 tgtttaagcg gcttctcgcg agaacaaaga ttttgcgtgg gtttagcgaa tgctggttat
     8821 ccacagtcgc acaagaaaaa cggggctgct gaggcgaccc ttgccctgag ccgaaatcgg
     8881 cgggtcaaac atgatgggga tagctggaac ctaatcttga tcgcggtgct tgcttcattc
     8941 cgttcgtttt acgctacgct ctccacgtca tgcgagggct tgacccgcgc atccatggtt
     9001 tagtcgagca cgacgtaaaa tatcacttga cgaacgtaaa gcgacgtttt acacatcaat
     9061 gtgatccgat cgttccgcag caaggccctt cgccgttatg ccgaaaccgg cgatgggtcc
     9121 aagctgagtg tccagaatcc ggatcgtatc cgcctgattc tggcgcggct cgacgcggcg
     9181 atcaggccac aagatatgaa cctccctggg ttgcgcttcc atgggctcaa gggacgtggc
     9241 aaaggacgat tcgccgtcga cgccagcggt cattggcgca tcacttttgc atgggacggc
     9301 gaagatgcga tcgacgtcga tttggaggac taccactgat gggcgctcaa ttgtccggcc
     9361 tcaagccgac gcaccccgga gagcttttgc gcgagatcgt gtttcccgcc ctcaaaatgc
     9421 cgaagcgtga gatcgcggac ctgctgggaa tctcccggca gatgctgcac ggcatcctcg
     9481 ccgagcgtca tccgatcacg cccaccatgg cgcttcgcat cggcaagctt ctgggcacga
     9541 gccccgagtc ctggctgcgg atgcaggaag cctacgatct gcgcaaggcg gagcaaacca
     9601 tgcggaaaga actcaaagcc attccaacgc tccggaccga ggccaccgag caagcgtaat
     9661 ccgcataagt gccggcgacg cgcgggcggc gccttcatgg cattcgcgga ctatttcctg
     9721 ttcgcgatgg ccgcgcctgt cttgcagggg ccggggctga cggtgtcatt cggctgcgaa
     9781 tttctcgacg cagcgcgcga gggcgaacgg gtcgaaggca gcggcgacat catgcgcgcc
     9841 ggtggctcca tgatcttttt gcgcggcgtt ttgaaatcgg cggagcgtcc gctgttcacg
     9901 ttttccggca ccatcaaacg cgtgaagcgg cgaatgccgg cgccttccgc cggttcaagc
     9961 tgacaactgc ttttcggcga tcgaaagcca ttcggtctgg gcctcgcgca ggtatttggg
    10021 cgcacgtctc atctgaatca gcagttcgtt gaaaactacc ctggcctcag cggtgcgccc
    10081 caccagatta agcaacagtc cgtaacgcac ccgcgcctcc gcgccgggga agtaggctgc
    10141 gaccgcatga aactcctcga gcgcctcatc gacgcgcccg actgcgccca gtgcccgggc
    10201 atagagcaaa tggccttccg cggattgaaa atccggccag cgcttttgca ggtcgtccag
    10261 cgtcgcgagc gcctcggccg gacgattgcg accgaactcg gccttcgcct tgcccagcgc
    10321 gtagaccgga tcgtcgccca gcggcagctg caagatgtga tcgtagtgac gttcggcttc
    10381 tccgaatcgt tcgattctca gacattcggc ggcaagcgca acgcggttgg cgatggtgtc
    10441 ggtgacggcg agccgatcgg ataattcacg atacagcttt tccggatcga gcctattggc
    10501 aacgcggcgg cgtgcctgtt gggcacccgg cccgctcagc atctccggca ccagttcgac
    10561 caatatgtaa gccaatgcgc cgatcccggg aatcatcagg atgatgaagg cccagggttg
    10621 caagcgccct gttctggatg cgtgatagac cagcgtgata tcgagcagga gtatgactaa
    10681 tgcgaacggc acggcgaatt ccagcgaagc ggtcgggtcg tattgtctgt cgccacgata
    10741 acccggcagg ttcgatcgaa caatcccggg ttgtcgctca tcgctcagtc ccgcacgctc
    10801 gtcgtgcgtc ctttcggatc ttctcttctg gttttattgc acgctgacca aagtcagatc
    10861 gacaaaccac gactgcggcg aaacaaaacc cttcaccttc gggctcatcg cgcgcggatt
    10921 gagatcgtgg acaatataaa gccagggcgg gttgtcgacg aggcgttcat gcgccttgcg
    10981 ataggcggtc gacatgactt tcggatcggt cgcctctgaa atcgtcttga gggcgtcctc
    11041 gaattcgtcg tctctccatt gctcgaaatt gaagccgttt ggcgagaaat tggcggccag
    11101 gaaatagcgc gccatgacgc cggcgtccga cgatggcgaa ctgatattga gcgccatggc
    11161 gcctcgcaga ttcgggctgt cgggcgtggc gcgggccgcc gtcagcagaa cctgccattc
    11221 gatcacgtcg aactccacgg tgacgccgca ggcctctttg aggttttgct gcaaaaattc
    11281 gttcatcggc aacggcaaca tttgccccga accggaattc gagatcagcg ccttgaacga
    11341 caatggtttt gctggcgtgt atccggcctc ggcgagcagc gccttgcctt tggcgggatc
    11401 gaatttgtag cggttgacgg ggttgccaaa atcgggatcg ccggtcttga gccatccggc
    11461 cgagggctca gcggtcccat tgagcaagga caccagccct tcccgatcga tgcaataatt
    11521 cagcgcctgg cgaactttca cgtccttgaa ggggctgttg gccgcgccga tgttgtagaa
    11581 ccagggccag acatgaggat atgagcccgt cgtaatggca aagccggccg atttcagcga
    11641 ggcgatgcca tcggccggcg gcacttcgat ccagtcgacc tggcccgagc gcaaggcggc
    11701 cagtcgcgaa ttcgcctcgg ggatcggcat cagcaccaca ttgtcgactt tcgccatctt
    11761 ggcggggtcc cagtacttat cccagcgcgc cagatccgcc tcctgccgcg gcacaatttt
    11821 ggtgatccgg aacggcccgg taccggcggc cggcaaggtc gcgaccttgg cccaatcccc
    11881 accggccttt tcgaacgagg cgggcgacgt gaacaaaagg tacaccgcca tataggggaa
    11941 gtaagatgcc ggcttggtcg tggtgatcgc gacggttgcg tcgtcgatct tcttgtagct
    12001 gcccatcacg ggaacgcggg cccgcgacat cgccgaactc ggcggttcga attgcgggct
    12061 ggtgctgttg aaatagcggt cgaggttcca gatcacggca tcggcattga aatccgtgcc
    12121 atcatggaat ttcacgccgt ggcgcagatg gaagatccag gtcttgttat cctccggatc
    12181 ctgctcccat ttctcagcta acccgggacg caaagccgcc aatcgatcgg tcctggtcag
    12241 atcccacagc accaatccct cgaagatcgg gtagccgagg aagcgcattc cctcaaagcc
    12301 gttgtttggc agtccggttg ccgtggggat atcggacgcc gtcatggcga tgcgcagcgt
    12361 ggtctcggcc attgcaggtt ggtgcgacag caagacggct gcgagcgcga cggtgcaggt
    12421 ggctttatga atcagcatga tatccctctg tcctacgatc ggacgatccg ctttcagcga
    12481 agtgcaggga attcaggagc aagtttcagg ccagccggag gcgccgcggt caatatccag
    12541 cggctgccct ttggtgtggc gccgccggac gatcatggtg atgggcgttt tgggcaatcc
    12601 tgcgcgcagg cagcggtgac aaccaatgcc aacgccgcaa caaccgacag caggcgcgca
    12661 gttgtagcgt tcaagccgcg cgctccagat gcgccatcag gcctgcaaac aggccgcgac
    12721 cgtcggtgca gcccatgatg tcttcgacgt ggttttccgg atgcggcatc atgccgagca
    12781 cgttgccgcg ctcgttgacg atgccggcga tcgattgcgc cgcgccgttg atattggatt
    12841 tttcgtccac caccccctcg gcggagcaat agcgatagag cacccgcccc tcgccttcga
    12901 gccgcttcag cgtctcttca tccgcctcgt aattgccctc gccatgcgcg accggcaccc
    12961 ggatgacctg cccggcatta tagccgcgcg tgaagggcgt gtcggagcgt tcgacccgca
    13021 gatgcacgtc atggcagatg aactttagcc gcgcgttgcg catgagcacg ccgggcaaca
    13081 ggccggcctc gcagaggatc tggaaaccat tgcagacccc gagcacgagg ccgccctggg
    13141 ccgcgaacgc gcgcaccgcg tccatgatcg gcgcccgcgc ggcgatggcg ccgcagcgca
    13201 gataatcgcc ataggaaaat ccgccgggca ccaccacgag gtcaataccc gcaggcagcg
    13261 ccgtctcggc gtgccagacc atctgaggct catggccgga gatcagtttc agcgcgcgcg
    13321 ccatgtcacg ctcgcgattg attccgggaa agacaagaac ggcggatttc atgtggctcg
    13381 cttgcctagc tcagaacttc cacccggtag ttctcgatca cggtattagc cagcagcttg
    13441 tcggcggcgt ctttcagcgc cgcttcggcc tcggccgcgt ccgagccgga tagctcgatg
    13501 tcgaacacct tgccctgccg cacgctggcg acgccatcga cgccgagcga tttcaacgcg
    13561 ccttcgatgg ccttgccctg cggatcgagg atgccggatt tcagagtaac cgtgacgcgc
    13621 gctttcaccg aaaacctcag ctcttcacca gcaccggacc cgagccgatc ggccgctcgt
    13681 tttccatcag gatgcccaaa cgcttggcaa cttcggtata ggcctccagc aggccgccga
    13741 gatcccggcg gaaacggtcc ttgtcgagct tctcgttcga tttgacgtcc cacagccggc
    13801 acgaatcagg cgagatctcg tcggcgacga tgattcgcat catttcgttc tcgaacaggc
    13861 ggccgcattc catcttgaaa tcgaccagac ggatgccgat cccgagaaac agcccggtga
    13921 ggaaatcgtt aacgcgtatc gccagcgcca tgatgtcgtc gatttcctgc ggcgtggccc
    13981 agccgaacgc ggtgatgtgc tcctcggaga ccatggggtc gttgagctgg tcgttcttgt
    14041 agtagaattc gatgatcgag cgcgg
//
LOCUS       DASWGM010000389         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2117402,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000389 DASWGM010000000
VERSION     DASWGM010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117402"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1045
                     /gene="radC"
                     /locus_tag="VGA15_11320"
     CDS             335..1045
                     /gene="radC"
                     /locus_tag="VGA15_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX9588317.1"
                     /translation="MPADTSDPQSPAETPHYHGHRMRLRERFHGAGPDALSDYELLEM
                     VLFAARARGDVKPLAKMLIKTFGSFAEVMHAPEARLREVDGVGDKTVTQLKLIAAAAS
                     RIAKGQLKQRTLLSSWDDVIDYCRTSMAFADKEQFRILFLDKRNQLISDEVQQVGTVD
                     HTPVYPREVIKRTLELSATAIILVHNHPSGDPTPSQADIHMTRAIIDIAGPLGIAVHD
                     HIIVGKNGHASMKGMRLI"
BASE COUNT          330 a          512 c          473 g          306 t
ORIGIN      
        1 caacttgaca ccagcgtcgg ggggtcagga ccacacgact tcgccgtccg caagggttgc
       61 gctctcgtca gcagcgccgc ctgcgtccac cgcattccgc cccgcgtccg tgacgatcgc
      121 gagccgcccc tctgtgggac gagaccgaat cgtaatattc ctgattctac ctggccgtta
      181 agcgaaattc ggatattccg aaatcggtcg ccacccagac ccggttccgt aaagcggcga
      241 ggtgcggaaa attccgcgct tcgcccgcca gccggattcg tcatgcggca attcgcggtt
      301 gcaaaacctg accggcacgg catggttaga accaatgccc gccgatacca gcgatcctca
      361 gtcacctgcc gaaacgccac attatcatgg ccatcggatg cggctgcgcg agcgcttcca
      421 tggcgccggc cccgacgccc tgagcgatta cgaactgctg gaaatggtcc tgttcgccgc
      481 tcgggcgcgg ggcgacgtca agccgctcgc caaaatgctg atcaagacat tcggctcgtt
      541 cgccgaggtt atgcacgcgc cggaagcgcg gctgcgtgaa gtcgatgggg ttggtgacaa
      601 gaccgtcacg caactaaagc tgatcgcggc ggccgcaagc cgcatcgcca aaggccagct
      661 gaagcagcgc actttgctgt cgtcatggga cgacgtgatc gattattgcc ggaccagcat
      721 ggcgttcgcc gacaaggagc agtttcgcat cctgtttctc gacaagcgca accagctgat
      781 atcagacgag gtgcagcagg tcggcaccgt ggaccacacc ccggtctatc cgcgcgaggt
      841 gatcaagcgc acactggagc tatcggccac cgccattatc ctggtgcaca accacccctc
      901 cggcgatccg acgccgtcgc aggccgatat tcatatgaca cgcgcgatca tcgacatcgc
      961 cggtccgctc ggcatcgccg tgcacgatca catcatcgtc ggcaagaacg gccatgcgag
     1021 catgaagggg atgaggctga tctgagatga gtcgccagat cagaagcgat cgcgggcgca
     1081 ccggatcatg atgacatcgc tgcgtcggcc gcctcggcgg tgcgctgcct cagcaggtct
     1141 tgttcgcgcc tgttgccccg ccccccgcag cggatttacg aacagcgcaa cgctttactc
     1201 ggatacctct agcagaattt ggactcctgc agtctgaaaa aatgttcggg cacgacggag
     1261 ggtggctcaa aaaaaatcgg gatagctcgg gatattttac gaccttccgg tgttttgttt
     1321 ggcagcgatc aagccccacc gcctggacgg cgatggtcaa tgcgcacgtt agagcgttag
     1381 ccaatacgct cgacgtaatg tctccaaaca actgtctcca atgcgcaaca tagtcttgaa
     1441 agggagtgca gagatgaaac acgtgttagc ctctctcaca atcggcgctt gcctgctgct
     1501 tccgtcggct ggtgtggttt tcgccacggg acaacccggc acctctgccg gggtgaattg
     1561 cgggctcgga agtgccacca tgacgcccgg ccatgcggct gcgtcgccag gtgctccgtt
     1621 c
//
LOCUS       DASWGM010000390         5496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_871825,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000390 DASWGM010000000
VERSION     DASWGM010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871825"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="VGA15_11325"
     CDS             complement(<1..1619)
                     /locus_tag="VGA15_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588318.1"
                     /translation="MTQPLLRIEDLHVTFSTRRGLVEAVRGVTLSLDAGEMLGLVGES
                     GSGKSVTGFATTRLLDAAGRVTAGRILFRGQDITKISDGDFRMLHGAAISMIFQNPRA
                     ALNPIRAIGQQIADAILSHKRMSREQAAGEALELLRAVQIRDPEKRMAAYPHELSGGM
                     CQRVMIAIAISCAPMLLIADEPTTGLDVTTQKVVMDLLARIAAQRGMATILITHDLGL
                     AARYCRRVVVMEQGRLVEEAAPQALFCNPRHVYTKRLVAASPTATSRIEDLVTDEERS
                     QYLAMLAACKPRPAPPPGTPLLLDVQSLAKRYDQGSPAVTDFSMTMAAGESVGLVGES
                     GSGKTTTSRMICRLIDSSEGVILFDGQSIGHLAARDFHRSPVRKEIQLVFQDPNDSLN
                     PRFTAFDCIAHPLLRLAGMRPGDALRQRVEECAQRVGLPLGLLPRFPHQLSGGQKARI
                     GIARAIACRPRLLVLDEPTAALDVSVQAVVLQLLDRLRREDNLAFLFVSHDLNVVRMM
                     CDHTIVLRNGCIVEQGESRAVFGNPQTAYTR"
     gene            complement(1616..2464)
                     /locus_tag="VGA15_11330"
     CDS             complement(1616..2464)
                     /locus_tag="VGA15_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588319.1"
                     /translation="MTGTTLRHAAWILRGNPLTAIAAAGVVLLIVIAVFGPQIVPYDP
                     IASNVSQALMPPSAAHLAGTDQLGRDTFSRLIVATRLDLVIAVSAVGISFVLGAVIGA
                     FCGYSGGRLDRTVGRFVDVMMAFPLFVLAMAMVAALGNRVENIVIATAVINLPFYIRF
                     ARAEVNIRRNVGWVEAARASGESPISVVLRFLLPNVLPAMAVQMSLNLGWAILNAAGL
                     SFIGLGVKPPTPEWGIMVAEGARFISTGRWWLVAFPGLALMLAVLCFNLLGDGMRDIL
                     DPRMRT"
     gene            complement(2461..3492)
                     /locus_tag="VGA15_11335"
     CDS             complement(2461..3492)
                     /locus_tag="VGA15_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588320.1"
                     /translation="MKSFGATMIWAGRRFLSSLPALFGVLVFTFLLMRVLPGDPAVFF
                     ASGPNAGKEEIAVIRKQMGLDKPVPNQLLLYLYDVGRGNLGRSIMTGQPVTTDLRNRL
                     PASLELTLTALLIALVSAIPLGVLAALKPGSVIDHGVRLFCALGVCVPTFVSGLLLIY
                     VFYYLLGVAPDPTGRLDVFTSLPPPRTGFLMIDFALAGDIDGWWAAFRQLILPAVTMA
                     LFVIAPLARMTRASMLVSLGSDFVRTARSVGLSWWRIVVTYALRNAILPVITIAGIVF
                     STMLGANVLVEKVYSWPGVASYALDALLSSDYAPVQGFVLLMASVFVVVNLLVDILYG
                     IADPRVSIG"
     gene            complement(3507..5099)
                     /locus_tag="VGA15_11340"
     CDS             complement(3507..5099)
                     /locus_tag="VGA15_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588321.1"
                     /translation="MFSAVAVLAFLLAAGPVSAQTRAETLRYVTGASVNTLDPNVPGT
                     TREAFAVSLSTYDRLVSFGRKQLNGKWVFDLDKITGELAESYDVSPDAMKITFHLRKD
                     AQFQDGKPVTAEDVKWSLDRAVTAPVLGKAQLLTGSMTSADQFKVIDPLTFEVTLPKP
                     DKLALPNLATVYPIIINSKVAKANATAEDPWAIAWLKEHTAGSGAYMIESFKPGEQVI
                     MKRNEAWNRGSPEKSAFFKRVIVQSVPEPATRANLVERGDADLVIDLQASDVQSLEGK
                     GTLKVVSTPQYNAVTFISMNNQIPPFDNANVRRAIAYALPYDDMFKAALFGRGTPLFG
                     AMWADGKPTTGAYPFQQPVKLDLERAKEYLKAAGMPDGFSTTFSFNVGQASTAEPMSA
                     LVKESLAKIGIKVDIQKLPDAQMSTQINEKKLPFFTEGIVAWLPSTDYFYRNFYTGEQ
                     RWNYSSIKNAELTAIAQQARFEPDKAKYDEYGKKLNAIHFAEMPQIPLWQPNQDAVMA
                     TSIDGYTYQFHRQVDYRDLHRK"
     gene            complement(5260..>5496)
                     /locus_tag="VGA15_11345"
     CDS             complement(5260..>5496)
                     /locus_tag="VGA15_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9588322.1"
                     /translation="LFAFASTMSFGPSGSSINAMTTAIPKLVHGLTRGLFRADLERHW
                     SSFKAYHVPQATIVRQTQAVEEQGTIGTGMKVRE"
BASE COUNT         1139 a         1685 c         1697 g          975 t
ORIGIN      
        1 tcgcgtgtgt aagcggtctg tgggttgccg aacaccgccc ggctctcgcc ctgctcgacg
       61 atgcagccgt tgcgcagaac gatggtgtga tcgcacatca tgcgaaccac attgaggtcg
      121 tgactaacaa agaggaaggc aaggttatcc tcgcgcctca acctatccag cagttgcagc
      181 accaccgcct gcaccgagac gtcgagcgcg gccgtcggct catccagcac caacaggcgc
      241 ggccggcagg cgatggcgcg agcgatgccg atgcgggcct tctggccgcc cgagagttga
      301 tgcgggaagc gcggcaaaag ccccaatggc aatcccaccc gctgcgcgca ttcctcaact
      361 cgctgtcgca atgcgtcgcc cgggcgcatg cccgcaagtc gcagtagcgg atgggcgatg
      421 caatcaaagg cggtgaaacg cgggttcaga ctgtcgttag gatcctgaaa gacgagctgg
      481 atttcttttc gtaccggcga gcggtggaaa tcccgcgcgg ccagatggcc gatcgattgc
      541 ccgtcgaaca gaatgacgcc ttcgctggag tcgatcaacc ggcaaatcat gcgcgacgtt
      601 gtggtcttcc cggaaccgga ttcgccgacg agcccgacgc tctcgccggc ggccatggtc
      661 atggaaaagt cggtcaccgc cggcgagcct tgatcgtagc gcttggcaag gctctgcaca
      721 tccagcagca gcggcgtgcc cggcggcggt gccggccgag gtttgcacgc ggcgagcatg
      781 gcgaggtact gacttctctc ctcgtccgtc accaggtcct cgatacgcga ggttgccgtg
      841 ggcgaggcgg ccaccaagcg cttggtgtag acgtgccgcg gattgcagaa aagggcctgt
      901 ggcgccgctt cctcgacgag gcggccctgt tccatcacca cgacgcggcg acaatagcgg
      961 gccgcgaggc cgagatcatg cgtgatcaga atcgtcgcca tcccgcgttg tgccgcaatc
     1021 ctggccagca ggtccatcac caccttctgc gtggtgacgt cgaggccggt cgtcggctcg
     1081 tccgcgatca gcagcatcgg ggcgcaggaa atcgcgatgg cgatcatcac ccgctggcac
     1141 attccgccgg agagctcgtg cggataggct gccattcgct tttcgggatc gcggatttga
     1201 accgcgcgca agagctccag cgcttcgccg gccgcttgtt ccctagacat ccgcttgtga
     1261 ctcaggatcg catcggcgat ctgctggccg atggcgcgga tcgggttgag cgcggcgcgc
     1321 ggattctgaa agatcatcga gatggcggcg ccatgcagca tgcggaagtc gccatccgat
     1381 attttcgtga tgtcctgacc gcgaaataga atcctgcccg ccgtcacccg cccggcggcg
     1441 tcgagcagcc gtgtggtcgc aaatccggtg acggatttgc ccgaaccgct ttcgccgacg
     1501 agcccgagca tttcacccgc atcgagcgag agcgtcaccc cgcgcaccgc ttcgacgaga
     1561 ccgcggcgtg tcgagaacgt aacgtgcagg tcctctattc gcagcagcgg ttgggtcatg
     1621 tccgcatgcg gggatcgaga atgtcccgca tcccatcgcc gagcaagttg aagcacagca
     1681 cggccagcat caacgctaac cccggaaatg cgaccagcca ccacctgccg gtcgaaatga
     1741 agcgtgcgcc ctccgcgacc atgatgcccc actccggggt cggaggcttg acgccaaggc
     1801 cgataaagga gagcccggcg gcgttgagaa tggcccaacc aaggttgagc gacatctgta
     1861 cggccatggc gggaagcaca ttcggcagca ggaaacgcag caccaccgag attgggcttt
     1921 cgccgctggc gcgcgccgcc tccacccagc cgacattgcg gcggatgttc acctccgcgc
     1981 gcgcaaagcg gatgtagaat ggcagattga tcaccgcggt cgcgatgacg atgttctcca
     2041 cccggttgcc gagcgccgcg accatcgcca tggccagcac gaacagcgga aaggccatca
     2101 tcacgtcgac gaaacggccg actgtgcgat ccagccgacc gccggaatag ccgcagaacg
     2161 cgccaatgac ggccccaagc acgaaggaga tgcccacggc cgaaacggcg atgacgagat
     2221 ctaatcgtgt cgcaacgatc agccggctga acgtatcgcg gccaagctgg tccgtgccgg
     2281 cgagatgcgc cgcgcttggc ggcatcagcg cctgcgacac attcgaggcg atcggatcgt
     2341 agggtacgat ctgcggccca aacacggcga taacgatcaa aagcacgaca ccggccgcgg
     2401 cgattgcggt cagcgggttg ccgcgcagga tccaggccgc gtgacgaagc gtcgtgcccg
     2461 tcatccgatc gacacccgcg gatcggcgat cccgtagaga atatcgacga gcagattgac
     2521 caccacaaac acactcgcca tcagcaacac aaagccctgg accggcgcat agtcagacga
     2581 cagcagcgca tccagcgcat aggaggcgac gcctggccag gaatagacct tctccaccag
     2641 cacattggct ccgagcatgg tcgagaatac aatgcctgcg atggtgatga ccggcaggat
     2701 ggcgttgcgc aacgcatagg tcacgacaat gcgccaccag gaaagcccga ccgagcgcgc
     2761 ggtgcgcacg aaatcgctgc ccagcgagac cagcatcgac gcgcgcgtca ttcgcgccag
     2821 aggcgcaatc acgaacaagg ccatggtcac tgccggcagg atcagctgtc ggaaagccgc
     2881 ccaccagcca tcgatgtccc ctgccaacgc gaagtcgatc atcaggaaac cggttcgcgg
     2941 tggcggcaac gaggtaaaga cgtcgagacg tccggtcgga tcgggtgcga caccgagcag
     3001 atagtagaag acgtagatca gcaaaagacc cgaaacaaag gttgggacgc agacgcccag
     3061 cgcacagaac aatcgcacac catggtctat gacggagcca ggcttcaacg ccgccagcac
     3121 gccgagagga atcgccgata caagcgcaat cagcagtgca gtgagggtga gctccagcga
     3181 ggccggcaac cgattgcgga gatccgtcgt gaccggctgc ccggtcatga tcgaccggcc
     3241 gagattgccg cggccgacat catagagata aagcagcaat tggtttggta ccggcttgtc
     3301 taatcccatt tgcttgcgga tgaccgcgat ctcctccttg ccagcatttg gaccggaggc
     3361 aaagaacacc gcgggatcgc ctggcagcac ccgcatcagc aggaaggtaa agaccagcac
     3421 accgaacagt gcaggcaggg aagacaggaa ccgcctcccc gcccatatca tcgttgcgcc
     3481 aaagctcttc atgaagcgcc ggccggttac ttgcggtgga ggtcgcgata atcgacctgg
     3541 cggtgaaact ggtaggtgta gccgtcgatc gacgtcgcca tcaccgcgtc ctgattaggc
     3601 tgccaaagcg gaatctgcgg catctcggcg aaatggatgg cgttgagctt cttgccgtat
     3661 tcgtcgtact tggccttgtc gggttcgaag cgggcttgct gtgcgatggc ggtcagctcg
     3721 gcatttttga tgctgctgta attccagcgc tgctcgccgg tatagaaatt gcggtagaaa
     3781 tagtcggtcg acggcagcca ggcgacaata ccttcggtga agaaaggcag cttcttctcg
     3841 ttgatctgcg tcgacatctg cgcatcgggc aatttctgaa tatcgacctt gatgccgatc
     3901 ttggcgaggg attccttgac cagcgccgac atcggttcgg ctgtcgaagc ctggccgacg
     3961 ttgaagctga acgtggtcga gaaaccgtcc ggcatgcctg ccgccttgag atattccttt
     4021 gccctctcga ggtcgagctt caccggctgc tggaagggat aagcaccggt tgtcggtttg
     4081 ccatctgccc acatcgcacc gaacaacggc gtgccgcggc cgaatagagc cgccttgaac
     4141 atgtcatcgt acggcagcgc ataggcgatg gcgcggcgca cgttggcgtt gtcgaatggc
     4201 ggaatctggt tgttcatcga aatgaaggtc acggcattgt attgcggcgt cgagaccacc
     4261 ttgagagtcc ccttgccctc cagtgactgg acgtcgctgg cctgcagatc gatcaccaga
     4321 tcggcatcgc cgcgctcgac caggttggcg cgggtcgccg gctccggcac cgattgcacg
     4381 atgacccgct tgaagaaagc ggatttttcc ggcgagccgc gattccaggc ttcgttgcgc
     4441 ttcatgatca cctgttcgcc cggcttgaag ctctcgatca tgtaggcgcc gctgccggcc
     4501 gtgtgctcct tgagccaggc gatcgcccag ggatcttccg ccgtcgcatt cgccttcgca
     4561 accttggagt tgatgatgat cggatagacg gtggcgagat tgggcaaagc gagcttgtca
     4621 ggtttcggca gcgtcacctc gaaggtcaag ggatcgatca ccttgaactg gtcggcgctg
     4681 gtcatcgacc ccgtgaggag ctgagccttg ccgagcaccg gcgcggtcac ggcgcgatcg
     4741 agcgaccact tgacgtcttc agcggtgacc ggcttaccat cctgaaactg ggcatccttg
     4801 cgcaaatgaa atgtgatttt catggcgtcg gggctgacgt cgtaggattc cgccagttca
     4861 ccggtgattt tgtcgagatc gaacacccac tttccgttga gttgtttgcg gccaaatgaa
     4921 accagccggt cgtatgtgct gagactcacc gcgaaagcct cgcgggtcgt acccggcaca
     4981 ttcggatcaa gcgtgttgac ggacgcgccg gtgacatacc gcagtgtctc ggcgcgagtc
     5041 tgcgccgata ccggccctgc ggcgagcaaa aaggcaagaa cagcgaccgc gctgaacata
     5101 ggcctgcagt acataactcg cattcttcaa tcccctgaat tcagcaagga agcctgcctg
     5161 agcagagtct caggatcgtg cggattctta agtgtgtaat ctcaagcaag taacgcgcca
     5221 accgaggctc cgatggccgg atcaagcgct ggcgccggat cattctcgca ccttcatgcc
     5281 ggttccgatt gtcccctgtt cctcaacggc ttgtgtctgc cgcacgatgg tcgcctgcgg
     5341 cacgtgatat gccttgaaag aactccagtg ccgctcaagg tccgcgcgga acaatccgcg
     5401 ggtcaggcca tgcaccagct tgggtatggc ggtagtcatc gcgttgatcg atgagccgga
     5461 tggaccgaaa ctcatggtgg aagcaaatgc aaacag
//
LOCUS       DASWGM010000391         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_164459,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000391 DASWGM010000000
VERSION     DASWGM010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164459"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VGA15_11350"
     CDS             complement(<1..885)
                     /locus_tag="VGA15_11350"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9588323.1"
                     /translation="MRNFHLAGRSTVHAQNAMVATSHPLASLTAIEVLRSGGTAADAA
                     VAACALLGVIEPQSTGIGGDCFALLQRKGEGKISAYNGSGRAPRAADASWYLERNMHA
                     IPLTSAHAVSIPGAIDAWATILRDHGKLGLDSLLQPAIKAAEEGYVVAPRIAFAWKNE
                     FDKLKKGTNTERYLLPHGKAPVAGDVIHQPELGQTLRAIASGGRDAFYKGAIAEDMVE
                     TLRGIGGLHTLDDFAAHTTETTTPIGTVYKGHDVWQCPPNGPGITMLVMLNILSRFDL
                     TKFPPMSVERFHLEAEAAR"
     gene            1065..1817
                     /locus_tag="VGA15_11355"
     CDS             1065..1817
                     /locus_tag="VGA15_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEX9588324.1"
                     /translation="MPHRVAAFYQFAALPDFRKLREPLRAVCAGLGLKGSVLLAHEGI
                     NGTLAGQPEAIVALVEELQCGALFGGRLDNLELKFSHAAAMPFQRLKIRLKKEIVTLG
                     DSRTDPTRQVGVYVDPADWNALIAGSDTLVIDTRNAFEVAMGTFPGAVDPGIRSFGQF
                     RDFAARHLDPAKHRRIAMFCTGGIRCEKASAYLLARGFTQVYHLKGGILRYLEGVPEA
                     ESRWRGECFVFDDRVALGHGLRERLTEQDSDE"
     gene            1810..>1922
                     /locus_tag="VGA15_11360"
     CDS             1810..>1922
                     /locus_tag="VGA15_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX9588325.1"
                     /translation="MSDMKALSERIDVLETRLAFQDETIETLNKTITAQWQ"
BASE COUNT          342 a          602 c          615 g          363 t
ORIGIN      
        1 gcgcgcggct tctgcttcga gatggaaccg ctcgacgctc atcggcggaa atttcgtcag
       61 atcgaagcgc gacaggatgt tcagcatcac cagcatggtg atgcccggac cgttcggcgg
      121 gcactgccag acgtcgtggc ctttgtacac cgtgccgatc ggcgtcgtgg tttcggtggt
      181 gtgcgcggcg aaatcgtcca gcgtatgcag gccgccgatg ccacgcaaag tctcgaccat
      241 gtcttcggcg atcgcaccct tgtagaacgc gtcgcggccg ccgctcgcga tcgcgcgcaa
      301 ggtctgcccg agttcgggct gatggatcac gtcgcctgcg accggcgcct tgccgtgcgg
      361 gagcagatag cgctcggtgt tggtgccttt tttcaacttg tcgaattcgt tcttccaggc
      421 gaaggcgatg cggggggcta cgacatagcc ctcttccgcg gccttgatcg cgggttgcag
      481 caggctgtcg agcccaagct tgccatgatc gcgcaggatg gtggcccagg catcgatcgc
      541 accggggatg ctgacggcgt gcgccgacgt cagcgggatc gcatgcatgt tgcgctcgag
      601 ataccacgac gcgtcggcgg cccgcggcgc gcggccggaa ccgttatagg ctgatatctt
      661 gccctcgccc ttgcgctgaa gcagcgcgaa gcaatccccg ccgatcccgg tggattgcgg
      721 ctcgatcacg ccaagcagcg cacaggccgc cacggcggca tccgcggcag tgccgccgga
      781 acgcagcacc tcgatcgccg tcaacgacgc cagcggatgc gacgtcgcca ccatcgcgtt
      841 ctgggcgtgg accgtcgacc ggccggcgag atggaaattc ctcatgccaa attgctcact
      901 ttgtcgttct accttgttcc cgaggcgccg ggttacatgg catattcatg aggcgatggg
      961 caatggtcgg gggcgcataa ctcaatatca gcgtcgtccc caaacacgtt cgcagggacg
     1021 aaaccggggt ttcctctccc ccaccctcct gataaaccac cgtgatgccg cacagggtcg
     1081 ctgctttcta tcagttcgcc gcgttgccgg atttccggaa gttgcgcgag ccgctgcgcg
     1141 cggtctgcgc cggacttggg ctgaagggca gcgtgctgct ggcccacgaa ggcatcaacg
     1201 gcacgttggc gggccagccc gaggcgattg tcgcactggt tgaagaattg caatgcggcg
     1261 ctctgttcgg cggccggctc gacaacctcg aattgaagtt ctcgcatgcc gcggcgatgc
     1321 cgtttcaacg cctgaaaatc cggctgaaaa aggagatcgt taccctcggc gacagccgta
     1381 ccgatccgac ccggcaggtc ggcgtgtatg tcgaccccgc cgactggaac gcgctgatcg
     1441 cgggttctga tacgctggtg atcgacaccc gcaacgcatt cgaggttgcg atgggcacat
     1501 ttcccggcgc cgtcgatccc ggcatccgga gttttgggca attcagggat tttgccgcgc
     1561 gccatctcga cccggcgaaa catcggagga tcgcgatgtt ctgcaccggc ggcatccgct
     1621 gcgagaaggc cagcgcctat ctgctcgcgc gcggttttac acaggtctat cacctcaagg
     1681 gcggcattct cagatatctc gagggcgtgc cggaagccga gagccgctgg cgtggcgaat
     1741 gttttgtgtt cgacgaccgc gtcgcgctcg gccacggatt gcgggagcgc ctgaccgaac
     1801 aggattccga tgagtgacat gaaggcgctc agcgagcgca tcgatgtact cgaaacgcga
     1861 ctggcgtttc aggacgaaac catcgagacg ctgaacaaga ccatcaccgc gcagtggcaa
     1921 ca
//
LOCUS       DASWGM010000392        27740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1979738,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000392 DASWGM010000000
VERSION     DASWGM010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 27740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27740
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979738"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VGA15_11365"
     CDS             complement(<1..891)
                     /locus_tag="VGA15_11365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX9588326.1"
                     /translation="MQMPLLKDFAQHVDTAHRQLSRLLSVVWYDLSWRFAPPGTFRQR
                     FEQAIVNMPQGICLYDAQDRLQLVNEQFCRIYGQPMETLHMGMRFYDILANSCSVGNY
                     PGRTVEDIYRERKAHIDSRRPDTFLQELGDGRLIAIYHQPLEDGGWVCTYEDITERRR
                     AESRIKFLAQHDVLTELPNRVLFNERLDEVLAMAREDSPSALLCLDLDGFKEINDDLG
                     HAAGDLLLKEVGRRLLANLRKGDTAARLGGDEFAILLQTASASEAVATAHRVTIALNG
                     KYELDQYGEANVGVSIGIACA"
     gene            complement(1108..1632)
                     /locus_tag="VGA15_11370"
     CDS             complement(1108..1632)
                     /locus_tag="VGA15_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588327.1"
                     /translation="MSPETVFRIHLVLGYVVWLLCFGVYVLPRLKSMDRVEAQRAIAT
                     LHSFRFFGLVFILPGVVGPNLPAGFAVFAAYGDFATGVLAILALLTVRIRPLFWAFAV
                     AFNLVGAADIILDYYHAIQVNLPALAGELGATYAIPIIYVPLLMITHCVAFYLLVRPR
                     PEVAQALVGDASAY"
     gene            complement(1644..1853)
                     /locus_tag="VGA15_11375"
     CDS             complement(1644..1853)
                     /locus_tag="VGA15_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588328.1"
                     /translation="MSEAQDPRQPPEALKPGSPSCASGPPPSPNLGVVMGPDFAQLSA
                     NLGRNLMEGRLGILTAVFEAASINT"
     gene            1931..2872
                     /locus_tag="VGA15_11380"
     CDS             1931..2872
                     /locus_tag="VGA15_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9588329.1"
                     /translation="MKQNFTVRQGALDGVEAFLGVAKHRSFRRAAAELGVSPSAISQA
                     VRALEVRVGAALFIRTTRSVGLTEAGERFLSRAKPAFEELVAASEVARELGQRPAGLL
                     RLSVPRAVVPILLEPLIASFCRAYPEVEVEIAASEELVDLAAEGFDAGLRLGQFIAAD
                     MVAVRLTPPFRFIIVGSPAYLARSDRPERPDDLRQHACVRLRRSGGALALWSLNDNGR
                     AIEIAVSGPLIANDFPTMLGAAVEGVGLAQVPEPIAAEAVTAGKLVHVLEPFAPMAPG
                     VFLYYPSRHQMMPKLRAFIDHMKSRSRAMRLARTKTS"
     gene            complement(3525..3929)
                     /locus_tag="VGA15_11385"
     CDS             complement(3525..3929)
                     /locus_tag="VGA15_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588330.1"
                     /translation="MKTALASGLAALIICLMSSGVGHAQSAGPPTPTTRILAIGTVNP
                     GADPAAARAILPTEVRETVKLYLDGKIDQWFSLQGRSGVAFILNVTDPVAAREMLEKL
                     PLGQAHLMTFELIPLAPLNPLRQLPGMTTGSP"
     gene            complement(3951..4604)
                     /locus_tag="VGA15_11390"
     CDS             complement(3951..4604)
                     /locus_tag="VGA15_11390"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9588331.1"
                     /translation="MKFDFESLNEDMRYKLMLATVLPRPIAWVTSQDRNGLLNAAPFS
                     FFNVFGTAPATVGIGVGRNSPTQPKDTCLNIRATEEFVVNLVPFSAAEKMRASSMPFP
                     SHVSEVAAIELQTSPSERVAPPRITESPASFECKFMQEIRLGNFSLILGRIVMLHVRD
                     EAVLDADRLHIDAANMDLIGRMEGALYTRTRDKFEPNVELAQALLAKHTGYGVCGIR"
     gene            complement(4684..4815)
                     /locus_tag="VGA15_11395"
     CDS             complement(4684..4815)
                     /locus_tag="VGA15_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588332.1"
                     /translation="MTILTIGTPDGFTVSFNLTEELSRELGEANLKGYGHHARTQTH"
     gene            complement(5054..5788)
                     /locus_tag="VGA15_11400"
     CDS             complement(5054..5788)
                     /locus_tag="VGA15_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588333.1"
                     /translation="MQELIKLSIEAHGGLDRWNQIRQISATFAPGGIGLKQRGQEAFT
                     RMPTRVTVDTREQKTIFGPFLAPGQCGIFEPNRTAVETVDGTVLEELKDPRHSFKSMP
                     AGTPWSATQLAYFAGYAMWMYLTVPFSLLRDGIECEEVEPWVEDGETWRALKVTFPKS
                     YVTHSTEQTIYFDDKGLIRRHDYTAEVSGDVQVAHYLYDHQKFDGIMFPTRRRVYPRG
                     PDLKPQKDFVVISADLSDFKLSRATP"
     gene            5753..6292
                     /locus_tag="VGA15_11405"
     CDS             5753..6292
                     /locus_tag="VGA15_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588334.1"
                     /translation="MGFNGQLDEFLHGISFFLNRLVEEAIHHQRNLIERLFNEIEHVR
                     QIATRFDRRAANVVAAVTLAATRAWLRTYESRTWPTPWSLALDQQAKWDERANRHQVV
                     GDKVRGRERQRIQRRFGLFDRLDGHEKNWSDLLSKPELLDLAIKIELKRRRHFARHVL
                     LPLFWCEGFDGSDGHDLHE"
     gene            6564..7529
                     /locus_tag="VGA15_11410"
     CDS             6564..7529
                     /locus_tag="VGA15_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9588335.1"
                     /translation="MRIAILALEGLFDTGLTVALDAFTLANKFSALQMGGAPRFDVSI
                     VGVRRRVRSGQGLVIPVQAITPDLKPDWVIVPALNTGRPEQLIPALERRDVIQAKMQL
                     RKWHAEGASIAASCIGTFLLAEAGLLDHQEATTTWWLAPLFRQRYPNVLLDESRMLVP
                     SDVGVTAGAAMGHLDLALWLIRKASPELASVVSRYLLADIRSSQAPYIIPNHLAQADP
                     LILRFERWAREHLKTGFSLQEAADALATSARTLQRRCEAVLGKSPLSYFQDLRVARAQ
                     SLLHGSGLDLDAIAAEVGYVDGATLRTLLRERLGRGVRELRANLR"
     gene            7962..8246
                     /locus_tag="VGA15_11415"
     CDS             7962..8246
                     /locus_tag="VGA15_11415"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9588336.1"
                     /translation="MATFTQLHSGNSTFTINPAIKKSMPFDTIKDFTPVAFIARSLLV
                     TASNKLPVKSTKELLTLAASKPGQITYASFGEFLSNEGAEAETMTPQQFS"
     gene            complement(8348..8410)
                     /locus_tag="VGA15_11420"
     CDS             complement(8348..8410)
                     /locus_tag="VGA15_11420"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588337.1"
                     /translation="MSDALPYTPDDIATQAIAAA"
     gene            complement(8473..9513)
                     /locus_tag="VGA15_11425"
     CDS             complement(8473..9513)
                     /locus_tag="VGA15_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588338.1"
                     /translation="MSATRFQIVRSTRFSRVGLPLLGIVLVGLALAPYWAGRAELRLL
                     AEIFAYLALASLWNLLAGYAGLASIGQQAYVGLGAYLLFGLAILVGVHPLLAIPLAGL
                     IAGVIAVPVALLIFRLKGAYFTIGTWVVAEVFRLFAFQVAALGGGSGVSLPIGVVTSI
                     APGRELREFALYWLSLVLLLVIMAAIFALLRSRYGLALTSVRDNEVAARSNGVDVWRI
                     KLMVYCACAFGTAMLGALIFLQKLRLSPDTAFSTNDWTAFIIFIVVIGGIGTIEGPIV
                     GTLVFFVLRQAFSDLGSVYLLMLGVVAIVIMMRAPRGLWGLVAARFGWKLFPLERRVQ
                     FVPSIANNLEFR"
     gene            complement(9513..10379)
                     /locus_tag="VGA15_11430"
     CDS             complement(9513..10379)
                     /locus_tag="VGA15_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588339.1"
                     /translation="MQWLNIVVQGVLTGGLYAMFAAGLSLIFGVMRLVNIAHGDLIVL
                     AAYLALVTTTTLGIDPLTSLVIVVPLMAAIGYALQKFLLNATLGDDLLPPLLVTFGIS
                     VIIQNGLLELFTADSQKLQAGPIEVQSLHLTSELAVGVLPLLQFGLAVAVIGGLQFVF
                     YRTAIGRAFRATSDDPAMAQLMGLDNRHIFALAMALSLAVVAIAGVLLAVRTNFDPAI
                     GPGRLIFGFEAVIIGGLGSLWGTLIGGIVLGVAQAIGANIDPGWQLLAGHIAFLIILA
                     LKPSGLFPKVQH"
     gene            complement(10384..11091)
                     /locus_tag="VGA15_11435"
     CDS             complement(10384..11091)
                     /locus_tag="VGA15_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588340.1"
                     /translation="MPDALLDIDGLDAFYGDFQALFGVSLRVETGDVVAVIGANGAGK
                     STLLKSIVGMAPPSRRGRICFAGEETSALPSFEVVKRGIAMVPEGRRLFPSLSVEENL
                     LVGGQLKRSGPWTLKRLYELFPMLGERRRQTPASLSGGQQQMVAIGRALMSNPALLLC
                     DEISLGLAPIVVRDIYTQLSAIAGEGVALVIVEQDIAQALQAARHVSCLQEGHVALAG
                     SSATITREAIATAYFGV"
     gene            complement(11084..11812)
                     /locus_tag="VGA15_11440"
     CDS             complement(11084..11812)
                     /locus_tag="VGA15_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588341.1"
                     /translation="MSSLLTLERISKRFGAIIIADSIDMTLNKGEALGLIGPNGAGKT
                     TLFGIATGTMSPNSGRVLLDNRDITGLRPEQRCRLGIARSFQVPQPFTGMTVFENLLV
                     AAAFGRDMRERDVHAHCVELLEQCGLAAKANWLAGSLPLLDRKRLELSRALATKPRVL
                     LLDEVAGGLTEHECAVLVELIREIRRTGVSIIWIEHIVHALLSVVDRLLVLHGGCFIA
                     QGDPAEVIKSQPVREVYMGIPADA"
     gene            complement(11833..13110)
                     /locus_tag="VGA15_11445"
     CDS             complement(11833..13110)
                     /locus_tag="VGA15_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588342.1"
                     /translation="MTQNTTPSRRKLLTTGLGVLAAPAILRVLPANAQGRTLRIGHVS
                     PRSGPLAAFGEADPFILGEIQRLIGNGIQNNGKTYKIEIISKDSQSNPSRASEVASEL
                     ILRDKVDLLVATSTADTTNPVADQAEVNEVPCITTDCPWQAYFFGRKGDPKKGFNWTY
                     HFFWGSEDLLAAFIALWDSLPTNKVVAGMFSNDADGNAFADPNMGFPPALAQAGYKLI
                     NPGFYQPLSNDFTAQIAAFKAANVEIVTGVFIPPDFATFWSQASQQGFKPKIVTVAKA
                     LLFPSAVAALGGRGNGLTTEVWWSPSHPFKSGLSGQTPKEMTDAYTQATKRPWTQPIG
                     FKHALFELAIDVFKRTKNLEDPKSILEAVRTTDYVSIVGPVRWSGQPVKNVTKTPLVA
                     GQWQGSGDKLDLVITTNKPAPNIPVGGTLQPLA"
     gene            complement(13185..13343)
                     /locus_tag="VGA15_11450"
     CDS             complement(13185..13343)
                     /locus_tag="VGA15_11450"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9588343.1"
                     /translation="MNIAVVGAGLMGHGIAQVFAVAGRIPALAAGAASCNPINRDAPL
                     EKGTNIAR"
     gene            complement(13358..14158)
                     /locus_tag="VGA15_11455"
     CDS             complement(13358..14158)
                     /locus_tag="VGA15_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9588344.1"
                     /translation="MTEWLKNPLSLFDVKGKSAVITGASGAFGALAAQVLAAAGARLV
                     LAAGNAKELAKSASDCQALGAEVETVLLRPSSEENCGAIIAKATERFGGVDILVVASG
                     KNDVAKINDMSPARFLDVMDANVTQSWLIARAAAKQMKEQGGGGKIVLMSSARGLLGH
                     PAGYTAYCASKSAVDGMTKALGCELGETGITVNAIAPTVFRSPLTEWMFEDNDRAKDV
                     RKGFLARVPKGRLGEPSDLAGPLLFLASKASDFYTGHILYADGGYTAG"
     gene            complement(14155..14502)
                     /locus_tag="VGA15_11460"
     CDS             complement(14155..14502)
                     /locus_tag="VGA15_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9588345.1"
                     /translation="MLVRRFNEAKAYEAPNHRNYTSLRLFGVDAGGPSGFVVGLSYFL
                     PGGGAGPDASPTEKVYVILSGELTVIVDGKETVLKANDSCFIGINEAREIINRGNDVV
                     TMLVAMSTSKAVS"
     gene            complement(14502..15347)
                     /locus_tag="VGA15_11465"
     CDS             complement(14502..15347)
                     /locus_tag="VGA15_11465"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9588346.1"
                     /translation="MIKVRGIEFQENANNEMGLEFYNLSHRFGYQSPNWPYFEDVKIE
                     RIHYMAKSGVLSQRITTSMHNTTHIDAPAHVVQGTPFIDEVPLPHFFGTGIVVSIPKK
                     KWEPITYDDLEKAAGKAVRPHDVVIVNTGYHHIYEDSEDYFLRAPGFVPSAADWFIEK
                     EVKVVGHDTQANDHPLATAIGPHGIGPIIQHVDLKKEYYEWSGGRDWKDDHPEWEPVH
                     RQLFKAGILGIENVGGDLDKVTGKRVTFAFFPWNWDRGDGCIIRLVAMIDNKGAYRIE
                     AGEAF"
     gene            complement(15496..16266)
                     /locus_tag="VGA15_11470"
     CDS             complement(15496..16266)
                     /locus_tag="VGA15_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9588347.1"
                     /translation="MKIVDTNEKSQVQVIARAAAILRALENENAGLSLGQIAQRVDLA
                     RSTVQRIIAALEVEKLVIAASPNGKMRLGPTILRLAASVRTDFASLARPFLVALSKEL
                     GETVDLSTIKKDQVVFIDQVTGPHRLQTVSVVGDAYPLYCTANGKAYLAQIDDAAIVE
                     LIGRKYPRRTKHTKTTFADLAVDLKTARQTGVAVDLEEHSSGVCATGVALRDPAGNYV
                     AISVPVPSQRFKAEYKTIARRLLETKGVLEGRIISQNL"
     gene            16537..17487
                     /locus_tag="VGA15_11475"
     CDS             16537..17487
                     /locus_tag="VGA15_11475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9588348.1"
                     /translation="MVRKIALEEHFLCPGFEKYWATTVGDVASDVLQRITARLSDFGD
                     DRIEAMDRAGVARAVLSLAGPGVQSEPDAAVAVRMAAAANDHLARELQKRPDRYAGFG
                     HLAMQDPTAAANELERCMRDLGFCGAMINGHTNGQYLDHPALAPFWERAAALSAPIYI
                     HPADPVTPSPVLDGHPALRRATWEWGFETGSHALRLVFGGIFARFPDARVILGHLGET
                     LPYLLWRFDSRAKLYGVELPRPPSSYIKSNIVVTTSGMFSAEPLNCAIAALGRDRVMF
                     SGDYPFESLEEAGHFMDHVALPDDVRADIAFNNASSILSL"
     gene            17607..18563
                     /locus_tag="VGA15_11480"
     CDS             17607..18563
                     /locus_tag="VGA15_11480"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9588349.1"
                     /translation="MTSRSASAQTAELKVSHFLPPNHTFQKAMVAWSDEIEKQSAGRL
                     KLRIYPAGQLGGGPNRQFDAARNGVVDIAISLHGATPGRYAVTELASLPFVSPSPGNT
                     SAVTSRRLSELAPEYLSKEHEGIRILWMAVTPPLMFHSKTPIRTIADFKGKRIRYAGV
                     QFKNIIDSLGAVPLLVPPQETQDGLAKGIIDAATFPYEGAASFDIATVAKYTLEPGVS
                     AATFAVVMNPAKYASLADDLKALIDKTTGPDAAAKFGASWDEAEQEGKTAMLAKGTQV
                     STLAKDQLELMKATFEPQVETAIGEIEGQGKPGRKFFEAYLK"
     gene            18641..19156
                     /locus_tag="VGA15_11485"
     CDS             18641..19156
                     /locus_tag="VGA15_11485"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9588350.1"
                     /translation="MNQQVSPPPQPPDLIQRLRTAQLRLAAVALVTMMTGTALDVFFR
                     YAFNKPLRFSYDLVESMLVIYVFHGISSVFLERRNIVIDVIDSLVSPRILRLLALIAD
                     IVSVMCLLLFLWGMLEPAMQAFQYGDRKLELGIPLYVLWLFALAGLTGTLACAIAAAA
                     HISLRQLRRQL"
     gene            19153..20457
                     /locus_tag="VGA15_11490"
     CDS             19153..20457
                     /locus_tag="VGA15_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9588351.1"
                     /translation="MSPGIVGALGILTLLVLLFVRIPVWAALALVGFAGNYILSGLQS
                     ALTLTGTSAFDVSSAYTLSVIPLFVLLGEVASNTRLSADLFNAARLLLSGVRGGLAIA
                     TIAASACFGAVCGSSIVTAATMTRIALPEMRKAGYDDRLATGSLAAGGSIGILIPPSI
                     ILVIYASIAEQSVPKLFAAALFPGLILTALYMAVAVGIAIAYPHYAPRMERASARERL
                     KALAGPWQFILLFIVTIGGIYAGIFSPTEAAAVGAFGSILLGALGRRLTWATLWRSIE
                     TSIRTSCMLFVIIIGANIFSSFMVQTQFPTMLADGASALNLSAPVVMVMIVLAYIVMG
                     CFLEGIGMVLITVPVFLPLVVKFGYDPIWFSILVVIVVEVGLIHPPVGMNLFVIQAQA
                     PDVRITSIYRGIIPFLIAPLLLIIILFLFPRLALWLPKLLYG"
     gene            20462..21433
                     /locus_tag="VGA15_11495"
     CDS             20462..21433
                     /locus_tag="VGA15_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX9588352.1"
                     /translation="MTNVLVLETYAEVYRDALRSEFHDLEIQVANSANVATVNFAEVD
                     VLIAFGMGVTDALFTRMPGLKWVQSLATGVDHFLRSKTLPAGVILTSGRGMHGPAMRE
                     TVLHLMLSVSHDSNRLVADKTARRWERRPWPLLAGKTAAVIGTGVAGSAIGAALQALG
                     MRVVGISGTPRDVDGFDVVLPRQELTKVAADADYLVNVLPADAVNLNLIGRKVFAAMR
                     PRAYFINVGRGETVDEAALIEALEQRRIAGAGLDVFATEPLPAASLLWEMPHVFIAPH
                     IGGLFEEYAQMAMPLIIRNMRAFRSGQTDEMINKVDRSRAATAETQP"
     gene            21430..22635
                     /locus_tag="VGA15_11500"
     CDS             21430..22635
                     /locus_tag="VGA15_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX9588353.1"
                     /translation="MSNTVDPASRILTRISDAELMRRWAAVRRVMQERGVDAIVMQNT
                     NDWLGGYVKWFTDLPANNGYPRTVVFHADAPMTLVEMGPSNVRRTYTADETLHRGVGE
                     MLHTPSFTSIAYTDQDDVQLAIEALRRNAKRAIGLVTPGTLPYAFVSALKEQLPATAF
                     VDVTADIDAIKAIKSDEEIALIRRTARVQDEVFAEVLKEIRPGLRDIDVTSIAQRKGQ
                     ILGSEQGIFLGGSSPLGIRSPFVPRYMQGRTLQRGDHLSLLIEINGPGGFYTEIARTI
                     VLGKATSELTDAFAAVRAAQGYSLSIMKPGVSAKEIFAKHNAYMTRNGMPAETRLYAH
                     GQGYDMVERPLIRQDDDMTLQQGMCLAVHPGYETPSLFAVICDNYLIEAHGPGDCLHQ
                     TAKTLFEID"
     gene            22837..23886
                     /locus_tag="VGA15_11505"
     CDS             22837..23886
                     /locus_tag="VGA15_11505"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX9588354.1"
                     /translation="MIPSFPMNAWYAAAWDVDIKHALFPRTICGKHIVMYRQADGQVT
                     ALEDACWHRLVPLSKGRLEGDSVVCGYHGLKYNASGRCTFMPSQETINPSACVRAYPI
                     VERHRFIWLWMGDPAIADPALVPDMHWNDDPAWAGDGKTIHVKCDYRLVVDNLMDLTH
                     ETFVHGGSIGNAAVTEAPFDVTHGDKTVTVTRWMRGIEAPPFWAKQLQKPGLVDRWQI
                     IRFEAPGTVNIDVGVAPANTGAPEGDRSQGVNGFVLNTMTPETETTCHYFWAFVRNHH
                     LTEQKLTTEIREGVAGIFREDEIILEAQQRAITENPDRAFYNLNIDAGAMWARRVIDR
                     MVAREIAPSRMHAAE"
     gene            23888..24646
                     /locus_tag="VGA15_11510"
     CDS             23888..24646
                     /locus_tag="VGA15_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9588355.1"
                     /translation="MSRERDPDRSVSHTVRAQLALRDLILSGRLRPGERISELWAVET
                     TGVSRTPVRMALVRLEEEGLLEAIPSGGFMVKAFTERDILDSIELRGVLEGLAARFAS
                     ERGVSARDLEPLKEALAELDRLVAQDSISVEAFSSYVTMNARFHALLTELSHSGPLIR
                     QIDRASALPFASPSGFVMAQSVLPEAHQILIIAQDHHRIVLDAIENREGARAEAVMRE
                     HARLAARNLRLALRNRTHLDLLPALALIRSPVAE"
     gene            24662..25642
                     /locus_tag="VGA15_11515"
     CDS             24662..25642
                     /locus_tag="VGA15_11515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEX9588356.1"
                     /translation="MRFELKWTKCTVDAIRDVTPAIREIVLRPEGFGIENYAIGSHVN
                     VAVLIDGRPETRCYSLVGDIAPDRYRIAVRRAPDSRGGSLHMWSLRPGATIEVSNPAS
                     LFEIDWSRKSFCLIAGGIGITPIIGISSALTRRNAEVELHYAVKSRHDAAFLDELSGS
                     LGDRLTIHASDEGKRINLDDTFGRLPADALVIICGPMRLLEAARRSWAAAGRVPTDLR
                     YETFGSSGLLPTEAFRVRIDNSGLEIVVSESKSMLAALNEAGYEVMSDCQRGECGVCA
                     IDVVSVDGQIDHRDVFFSDQQKKSNGRICPCVSRAVGAITVDTLYRNDAL"
     gene            complement(25701..26750)
                     /locus_tag="VGA15_11520"
     CDS             complement(25701..26750)
                     /locus_tag="VGA15_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588357.1"
                     /translation="MEPTRYHTETKTRASSVLALAGVVLVLIFAALPAFAGRGLIQDL
                     IFVFYMLALAQCWNMLAGYAGLVSVGQQAFVGLGGYLLFALTIGADIDPIAAVVLSGL
                     IAAAFAVPTAFVVFRLRGPYFAIGSWVVAEVYRLVFAQFKQLGGGTGTSLPPSATNEI
                     FGIEWVKVMFEVRSPAARDIITYWIALALAAGAPLLVYLVLRSRYGLALFAIRDSEAT
                     AETSGVDSFRTKLGVYVLVAAATGMIGALIYLQKARISPDAAFSVLDWSAYVIFIVII
                     GGVGTIEGPIIGVMVFYTMQHYLADFGTWYLILLGALAIVIMLFAPKGIWGYVAQRYH
                     IALFPVRRMLVRPNE"
     gene            complement(26784..27653)
                     /locus_tag="VGA15_11525"
     CDS             complement(26784..27653)
                     /locus_tag="VGA15_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003554581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588358.1"
                     /translation="MLDWLDTIIQGVLLGGLYALFAAGLSLIFGIMRLVNLAHGDLIV
                     LAAFVILALATWFGFDPFLAALLAMPAMFAAGFALQHVLLNRTLGRDLLPPLLVTFAL
                     SIIIQNGLLEAFTADSRRLRLGAIETASIHLGGNLAIGVMPLLTLGSAIAVILLLNLL
                     FYRTALGRAFRATSDDLEIAQLMGIDHHRIYSIAMGIAMVVVTIAALYLGIRANFDPT
                     IGPARLIYAFEAVIIGGLGSLWGTLAGGIIIGLAQTVGAKLNPEWQILAGHIAFLFVV
                     VLRPRGLFPRAVD"
BASE COUNT         5602 a         8502 c         8289 g         5347 t
ORIGIN      
        1 cgcgcaggca atgccaatgc tgacaccaac gtttgcttcg ccgtattggt cgagttcata
       61 tttgccgttc agcgcaatgg tcacgcggtg cgccgtggcg acagcttccg aggcggaagc
      121 ggtttgcaac agaatagcga attcgtcgcc acccagacgg gccgccgtgt cacccttgcg
      181 caaattggcg agaagacggc gcccgacttc cttgagcagc aggtcgccgg cggcgtgacc
      241 aaggtcatca ttgatctctt tgaagccgtc caaatccagg cagagcagcg cggacgggct
      301 atcctctcgc gccatcgcca gcacttcgtc gagacgctca ttgaacagaa ctcgattcgg
      361 cagctcggtc aggacatcgt gttgcgcaag gaacttgatg cgggattctg cgcgccgtct
      421 ctcggtgata tcctcatagg tacaaaccca tccgccgtcc tcgagcggct gatggtagat
      481 tgcaatcaat cgaccgtcgc cgagctcctg aaggaaagtg tccggccgac ggctgtcgat
      541 gtgcgctttc cgctcccggt agatatcttc gaccgtgcgt cccggataat tgcccacgga
      601 acagctattg gcgagaatat cgtagaagcg catccccatg tgcagcgtct ccatcggctg
      661 accatagatg cggcaaaact gttcgttaac caactgaagc cggtcctggg cgtcgtagag
      721 gcagatgccc tgaggcatgt tgacgatcgc ctgctcgaag cgctggcgaa aagtccccgg
      781 tggggcaaag cgccaggaca gatcgtacca cacaacggaa agcagccggg acagctgacg
      841 atgagccgta tcgacgtgtt gtgcgaagtc tttcaatagc ggcatctgca tgagcgaagt
      901 tgcaacacaa tgaaaaaggc aatcgctcaa ctcaatcagg aaaggcttct gatttgctta
      961 attcccgcca gttcgacccc aacggaatcg caagctacgg ttcgaaaccg aaatgaaacc
     1021 aagtcgtgac tattcgtaac gcaaagtgct ctgattatag gcatcgatgc ggccggccgg
     1081 atctgtgaag gacaatggcc atccgatcta gtatgcggag gcatcgccga cgagggcctg
     1141 cgccacctcg ggccgaggac gcaccagcaa atagaacgcg acgcagtgtg tgatcatcag
     1201 caggggcacg tagatgatcg ggatcgcgta tgtggcgccc aactcccccg ccagcgcagg
     1261 aaggttgacc tggatggcgt ggtaataatc gaggatgatg tcggccgccc ccacgagatt
     1321 gaaggcgacg gcgaacgccc aaaacagcgg acgtattctt accgtgagaa gcgccaggat
     1381 ggccagcacc ccggttgcga agtcaccata ggcggcgaag acggcgaagc cagccggcag
     1441 attggggccg acgactccgg gaaggatgaa gacgagcccg aagaagcgga agctgtgtag
     1501 ggtggcgatg gcgcgttgcg cttcgacccg gtccatcgac ttgagcctgg gcaggacgta
     1561 cacgccgaag caaagcagcc acacgacata acctagaacg agatggattc ggaacacggt
     1621 ttcaggcgac atttggcctc ctatcaggtg ttaatggacg cggcctcgaa caccgcggtc
     1681 aggatgccga gccggccttc catgagattt cgtcccaagt tggcggaaag ctgcgcgaag
     1741 tccggcccca tcaccacgcc gagatttggc gaaggcggcg gccccgacgc gcagctgggc
     1801 gatccaggct ttaaagcttc aggtggttgt cgcggatcct gggcctccga catcggcgtg
     1861 gctcctgatt tgcaagttag atgtacgacc gcttcccgcg agcgactatt cgcgataatg
     1921 tcgacaggct atgaagcaga acttcacagt caggcaaggc gctctcgacg gcgtggaggc
     1981 attcctgggc gttgccaagc accgcagttt tcgcagagcg gccgcggaac tcggggttag
     2041 tccatcggcg attagccaag cggtacgcgc acttgaggtg cgcgtcggcg cagcgctgtt
     2101 catacgcaca acgcgcagtg tcggcctgac cgaagccggc gaacgattcc tttcgcgcgc
     2161 aaagcccgcc ttcgaggagc tcgtcgccgc gagcgaggtc gcgcgtgaac tcggacagcg
     2221 gccggccgga ttgttgcgcc tctcggtgcc gcgcgcggtg gtgccgatcc tgctggagcc
     2281 gctgatcgca tccttctgcc gggcctatcc cgaggtcgag gtggagatcg ccgcaagcga
     2341 ggagctggtc gacctcgcgg ccgaagggtt tgacgccggc ctccggctgg gccagttcat
     2401 cgccgccgac atggtcgcgg tgcggctgac gccgccgttt cgcttcatca tcgtcggtag
     2461 ccccgcctat ctcgcccgca gcgaccggcc cgagcgcccg gacgatttgc gccagcacgc
     2521 atgcgtgaga ctgcgacgat caggcggcgc gctcgcgcta tggtcgctca acgacaacgg
     2581 ccgcgcgatc gagattgccg tatcgggacc gctcatcgcc aacgactttc ccaccatgct
     2641 tggcgcagcc gtcgaaggtg tgggtcttgc gcaagtgcct gagccgatcg cggccgaagc
     2701 ggtgaccgcg ggaaaactcg tgcacgtgct ggagccgttc gcaccgatgg cgccgggtgt
     2761 gtttctctac tatcccagcc gccatcaaat gatgccgaag ctgcgagcct tcatcgatca
     2821 catgaagagc cgttcacgcg ccatgcggct tgccagaacg aagacttctt gacagctcga
     2881 gccattgaaa acgatggagc gggtgaagcg ctctgactca aatattttga tctgttttta
     2941 tcaatgatcc caatcgctta tgcttgtcgg caattttcgc gttggacgtt ttgttggacg
     3001 tttcattgga cactccttcg ccgctcaact tggctcgtgt gccactcaaa acccgtttcc
     3061 gtctctatgg aaatccgtga agtattggtg gcaacgcctg tatcagcaac acggcgcttc
     3121 cgcccaacac gctggcacct acgacgacca ggggacagga cgcgccccca aagcatctcg
     3181 ttgctggcga cgccattcga atttggcatc tgtgtgcacc ccgctttttc gtgcgacgaa
     3241 gacgtcatag actgagtcgc tgggtcgccg atgttgacaa tattgagcct acggcgtgga
     3301 agcacgtcgc gggctcgatc gttagcctcc aatcgggtcg ccggtttacg ccgtgtcgtg
     3361 tctcgatgag gttcttaagc tccgcccatt ccggagtgac caccgtcggg gttcttgcag
     3421 aagaaccgcc tccagagatc cgtacgagcc gtaagcctct tcgaacagac aaggggctgt
     3481 tgcgacgcag cggacaaact acggacgatc ggctggcggc ctccctaagg cgaacccgtt
     3541 gtcattcccg gcaactgtcg aagtggatta agcggcgcaa gagggatcaa ctcaaaggtc
     3601 ataagatgag cttggcccaa gggcagtttt tcgagcatct cgcgggcggc tacgggatcc
     3661 gtgacgttta atatgaacgc cacgccgctc cgtccctgaa gcgagaacca ctggtcgatc
     3721 ttgccgtcca ggtagagctt tacggtctcc cggacttcag tcggcaggat cgcgcgcgct
     3781 gctgccggat cggcaccggg gttcacggtt ccgatggcaa ggattcgtgt ggtgggcgtt
     3841 ggcggacctg ccgactgtgc gtgcccgacg cctgaactca tcaggcagat gatcaacgca
     3901 gcaagaccgg aagcgagagc agttttcatt ttgtcctcct catgtgttct ctatcgaatg
     3961 ccacacaccc catatcctgt gtgctttgcc agtagcgcct gcgccagctc gacgttcggt
     4021 tcgaacttgt cacgggtgcg agtgtaaagc gcgccctcca tacggccgat gaggtccatg
     4081 ttcgccgcgt caatatggag acgatcggca tcgagcacgg cttcgtcgcg aacgtgcagc
     4141 atgacaatgc ggccgaggat caggctgaaa tttccgagcc tgatttcctg catgaacttg
     4201 cattcgaagg aggccgggct ttcagtgatc ctaggcggag ccacccgttc actcggcgat
     4261 gtctgaagct cgatggccgc gacctcgctt acatgcgacg gaaacggcat agaactcgcc
     4321 cgcatctttt ccgccgcgga gaacggaacg agattgacca cgaattcctc cgtcgcgcgg
     4381 atgttgagac acgtgtcctt gggctgcgta ggagagttcc ggcccacgcc tatcccgacc
     4441 gttgcaggcg ccgtaccgaa cacgttgaag aaggaaaacg gtgcggcgtt gagcagtcca
     4501 ttcctgtcct gggaggtcac ccacgcgatc ggccgcggca acaccgtcgc cagcatcagc
     4561 ttgtaccgca tatcctcatt gaggctttcg aagtcgaact tcatttgcaa tctcccatgc
     4621 gggcttagga atgtatcgcg gcgctggact gaacgacgac ctgcgattct cgagaatctc
     4681 cggtcagtgc gtttgcgttc tggcatgatg accgtagcct tttaagtttg cctcgccgag
     4741 ttcacgcgaa agttcctcgg tgaggttgaa cgaaaccgtg aatccgtccg gcgtcccaat
     4801 tgtcaggatg gtcattgggg ccaagctcaa ttcgaaactc cctcagcgga tacgtgatgc
     4861 gcaggattgc cgtggaggaa caagatcgga gcgccagtgc cacgctcgtg atatgccatc
     4921 tggcgaccgg tcgcactgac agcgttcttt tcaagcggct gttcggtcat ggtgagatct
     4981 ccaataagca gcttcgtcgg gcgtaaattc gccgtccggc atggcgatcg tgttgattgc
     5041 aattcggcga actctaagga gtcgcgcgcg agagtttgaa gtcgcttaga tcagccgaaa
     5101 tgaccacgaa gtctttttgc ggcttcaggt cggggccacg aggataaaca cggcgcctgg
     5161 tgggaaacat gatcccatcg aacttttggt gatcgtagag atagtgcgcg acctgcacat
     5221 cgccggaaac ttcagccgtg tagtcatgcc tacggatcag ccccttgtcg tcaaaataaa
     5281 tggtctgctc tgtggaatgc gtgacgtagg acttcgggaa ggtgactttc aatgcgcgcc
     5341 acgtttcgcc gtcttccacc cacggctcga cttcctcaca ttcaatccca tctcttaaaa
     5401 gagagaacgg cacggtgagg tacatccaca tcgcgtaacc ggcgaagtaa gccagttggg
     5461 tcgcgctcca cggcgtccca gcgggcatgc tcttgaagga gtgccgtgga tctttcagtt
     5521 cttccagaac ggtgccatcg acagtctcaa cggctgtgcg atttggctcg aaaatgccgc
     5581 actgcccggg agccaggaac ggtccaaaaa tcgttttttg ctcacgcgta tccacggtca
     5641 cccgcgttgg catccgcgta aaggcttctt ggcccctctg cttcaaacca atgcccccgg
     5701 gggcaaaggt cgcggagatt tggcgtatct gattccatcg atcgagcccg ccgtgggctt
     5761 caatggacaa cttgatgagt tcctgcatgg gatatccttt tttctaaatc ggctcgtcga
     5821 agaggcgatc catcatcagc gcaatctcat cgagcggctc ttcaatgaga tcgagcatgt
     5881 caggcagatc gccacgcgct tcgacagacg cgccgccaac gtcgtcgccg ccgtaaccct
     5941 ggcggcgaca cgcgcatggc tcagaactta cgagtccaga acctggccga cgccctggtc
     6001 acttgccctg gatcagcagg ccaagtggga cgagcgggcc aaccggcatc aggtcgtagg
     6061 tgataaggtt cgcggccgtg agcggcagcg tatccagcgc cgctttggcc tgttcgaccg
     6121 actcgacggt catgagaaaa attggtccga ccttctctcg aaaccagaac tgctcgatct
     6181 tgccatcaag atagagctta agcgtcgcag gcacttcgct cggcatgtgc tgctgccgct
     6241 gttctggtgt gagggatttg acggttccga cggccatgat cttcacgagt gatctccttg
     6301 tctgacgttc attgcgttga cgaaagctga tttagctggg gagcccaaag gctggtagtg
     6361 tcgtatcagc caactttaat gtcacttacg acattttaga tttggctctc ataggggaaa
     6421 aaaacctcgt ttcatcagct agatgattgt ctttgttgat ttcttttgct cgtctccgtt
     6481 ccgatgttcg acggagaatt atctagccgg tggatcgcaa aagaggtatc gtcgtaactg
     6541 acaattttga ggccatttac gccatgcgta ttgcgatact cgctcttgaa gggttgttcg
     6601 acacggggct caccgtggcg ctcgacgcgt tcacgctcgc caataaattc tcggctctac
     6661 aaatgggcgg ggcaccccgt tttgacgtgt cgattgtagg cgtgcggcga agggtccggt
     6721 ccggccaggg actggtgata cccgtgcaag ccataacgcc agatttgaag ccggactggg
     6781 tgatcgtgcc ggcgcttaat accgggaggc cggagcagtt gatcccggct ctcgaacgac
     6841 gggatgtcat tcaggccaag atgcagctgc ggaagtggca tgccgagggc gcttctatcg
     6901 ccgcttcatg tatcggcacg ttccttctgg cggaagccgg acttctcgat catcaggaag
     6961 caacaacgac ctggtggctc gctccgctgt ttcggcaacg ctacccgaat gtcttgctcg
     7021 atgagtctcg gatgctagtg ccatcagacg tgggtgttac ggctggcgcg gcgatgggac
     7081 atctcgatct cgcgctctgg ctgatacgca aggccagccc ggaactggca tccgtcgtct
     7141 cacgctacct gctcgccgac attcgctctt cgcaagctcc ctatattatt ccgaatcatt
     7201 tggcgcaggc tgatccactc attctgcgct tcgagcggtg ggcgcgcgaa catctcaaga
     7261 cagggttctc actgcaggaa gccgccgatg cgctggcgac gagcgcacgc acgttgcaac
     7321 gccgctgcga agcggtgcta ggcaaatcgc cgctttccta tttccaggat ctccgtgtcg
     7381 cacgcgcaca atctctgctc cacggcagcg gcctcgacct cgacgccatt gccgccgagg
     7441 tcggttacgt cgacggagca acgttacgaa cgctcttgcg cgaacgcctg ggacgaggcg
     7501 tgcgcgagct tcgcgctaac ctgcgctgac ggcctgctga ctgtttggca acgcagctac
     7561 cctctgcggc gccgaaacgt tcctgcgtta aacagatgcc gccggaatgc ccgcattggg
     7621 tcacgagcgg cgatcaaggc aggtaccagc aaagcccggc ttgcctcggg cggccgaaat
     7681 gatgacccct tgtagcgacg atcaacaagc gaggtcgctt ccgagagtct gaagggcctt
     7741 gtggggacaa gatttgaacc tacgtgaacc tggagagccc tgccgtcgag agttgccgaa
     7801 ctggactggt cgacaagaag tctgcgagtg tttctcccgc ctgagcgagc ttccatatat
     7861 tcgtctttcc cggatcagat ccacccccat ttggaagcca aatcgtcaca tcggcatttt
     7921 cagtgcacac atagtgcaca cgaaaacccg agcgcacaca catggcgacc ttcactcagt
     7981 tgcactctgg aaactcgacc ttcacgatca acccggcgat caagaagagc atgccgttcg
     8041 acacgatcaa ggacttcaca ccggtcgcct tcattgctcg ctcgctcctg gtcaccgcgt
     8101 cgaacaagct gccggtgaaa tccacgaaag aattgctcac tctagctgcc agcaaaccgg
     8161 gccaaatcac ctacgcctcg ttcggcgagt ttctgtcaaa tgagggcgcc gaagccgaaa
     8221 ccatgactcc gcagcaattc agttgattgc tcgatgcgcg gcagacggtg catgaagaca
     8281 tccggcgccg gcgttggact cccgttttgc gggtcgcgcg caaccgatgc aggatggacg
     8341 ctccggtcta agccgctgcg attgcctgcg tcgcaatatc gtcaggagta tagggcaacg
     8401 catcagacat tgtcggcgtg tgaatgccgc gcgtcaccgt gcaggtgatg aagatcttgt
     8461 tgcgcttttt gattatcgga attccaaatt gtttgcaata gatggaacga attgaacccg
     8521 tcgctcgagc gggaacagtt tccagccgaa ccgggctgca acgagtcccc acaagccgcg
     8581 tggcgcccgc atcatgatga cgatagcgac gacgcccagc atcaggagat agacgcttcc
     8641 gagatcggag aaggcctgac gcagcacgaa gaagaccagc gttccgacaa tcggcccctc
     8701 gatcgtgccg atgccgccga tgacgacgat gaagatgatg aaggcggtcc agtcgttggt
     8761 gctgaacgcc gtatctggcg agagccgcag cttctgcaga aagatcagcg ctcccagcat
     8821 cgccgtgccg aaggcgcagg cgcaatagac catcagcttg atgcgccaga catcgacgcc
     8881 attgctgcgc gcggcaacct cgttgtcgcg cacggatgtg agcgcgaggc cgtatcgtga
     8941 gcgcagaagc gcaaagatcg cggccatgat gaccagcagc aagaccagcg agagccaata
     9001 gagcgcaaat tcgcgcaact cgcggccggg cgcgatcgat gtaaccaccc cgatcgggag
     9061 actcactcca gagccgccgc cgagcgccgc gacctgaaat gcaaacagac gaaacacttc
     9121 cgccaccacc caagtgccga tcgtgaaata cgcgcccttc aggcgaaaga taagcaatgc
     9181 caccggcacc gcgatgacgc ctgcgatcag accggccagc ggaatggcga gcaatggatg
     9241 gacaccgacc agaatcgcca ggccgaacaa aagataggcg ccgaggccga cataggcctg
     9301 ctgccctatc gaagcgaggc cggcatagcc ggcaagcaga ttccacaggc tggccagcgc
     9361 cagataggcg aagatctccg ccagcagcct cagttcggcg cgaccggccc agtacggagc
     9421 cagcgcaagg ccgaccagga cgatcccgag caacggcagg ccgacacggc tgaaacgggt
     9481 cgagcgcaca atctgaaatc gagtggcact catcaatgct ggaccttggg aaacagaccg
     9541 ctcggcttga gcgcaaggat gatcaggaat gcgatatgtc cggccagcaa ttgccagccg
     9601 ggatcgatat tggcgccgat cgcctgcgcg acacccagca cgatgccgcc gatcagcgtg
     9661 ccccaaaggc taccgaggcc gccgatgatc accgcctcga acccaaagat cagccgcccc
     9721 ggaccgatcg ccggatcaaa attggtgcgc acggcgagca acaccccagc aatagcgacg
     9781 accgcaagcg agagcgccat cgccagcgcg aagatgtgac ggttgtcgag gcccatcagc
     9841 tgcgccatcg ccggatcatc ggacgtcgcg cggaacgcgc ggccgattgc tgtgcgatag
     9901 aaaacgaact gcaggccgcc gatgaccgcg acggcgagac cgaactgcag cagcggcagt
     9961 acgccaacgg cgagttcgct ggtcagatgc aggctctgca cctcgatcgg cccggcctgc
    10021 agcttctggc tatcggcggt aaacagctcg agaagcccgt tctggatgat caccgaaatc
    10081 ccgaaagtca ccagcaatgg cggcaacagg tcgtcgccga gcgttgcatt cagcagaaat
    10141 ttttgcagcg cgtagccgat cgctgccatc agcggcacga cgatcacaag cgacgtcagc
    10201 ggatcgatgc caagcgtggt tgtggtcacg agggcgagat aggctgcgag cacgatgagg
    10261 tcgccgtgcg caatgttgac taggcgcatc acaccgaaga tcagcgaaag ccccgccgcg
    10321 aacatggcgt acagtccgcc ggtgagcacg ccttggacca cgatattgag ccactgcata
    10381 ctgctagacg ccgaaatacg cagtggcaat cgcttcgcgc gtgatcgtag ctgatgatcc
    10441 ggcgagcgcg acatggcctt cctgcagaca ggagacatgc cgcgccgcct gcagcgcctg
    10501 cgcgatgtcc tgctcgacga tcaccagcgc gaccccctcc ccggcgatgg cggacaactg
    10561 ggtatagatg tcgcgcacca cgatcggtgc gaggccgaga ctgatttcat cgcacagcaa
    10621 tagcgcagga ttcgacatca gcgctcggcc gatcgccacc atctgctgct gcccgccgga
    10681 gagtgacgcc ggcgtctgcc ggcgccgttc gccgagcatc ggaaacagct cgtaaagccg
    10741 cttcaaggtc cacggcccgg atcgcttcag ctgtccgccc accagcaggt tctcctcgac
    10801 gctgagcgaa ggaaacagcc ggcggccttc cggcaccatc gcgatgccgc gtttcaccac
    10861 ctcgaacgac ggcagcgccg atgtctcctc gccggcaaag cagatcctgc cgcgccgcga
    10921 cggcggcgcc atccccacga tgctcttcag cagcgtgctc ttgccggcac cgttcgcgcc
    10981 aatcacggcg accacgtcgc cggtctcgac ccgcagcgag acgccgaaca gcgcctggaa
    11041 gtcgccgtag aaggcatcga ggccatcgat gtcgagaagg gcatcaggca tcggccggaa
    11101 ttcccatgta gacctcgcgc accggctggc tcttgatcac ctctgccgga tcgccctgtg
    11161 caatgaagca gccgccgtgc agcacgagca gccggtcgac caccgaaagc agggcatgca
    11221 cgatgtgctc gatccagatg atcgacacgc cggtccttcg gatctcgcgg atcaattcga
    11281 ccagcacggc gcattcatgt tcggtcaacc cgcccgccac ttcgtcgagc agcaggaccc
    11341 gcggcttagt cgcaagcgcc cgcgacagtt cgagacgctt gcgatcgagc agcggcagcg
    11401 agccggcgag ccagttcgcc ttcgccgcca gtccgcattg ctcgagcagt tcgacgcagt
    11461 gcgcatggac gtcgcgctca cgcatgtcgc gcccgaaggc ggcggccacc agtagatttt
    11521 cgaagacggt cattcctgta aacggctgcg gcacctggaa tgagcgtgcg attcccaacc
    11581 ggcagcgctg ctccggcctc aacccggtaa tatcccgatt gtcgagcaac acgcgtccgg
    11641 aattgggaga catcgtgccg gtcgcgatgc cgaacagcgt cgtcttgccg gcgccattcg
    11701 gcccgatcag ccccagcgcc tcgcccttgt tgagcgtcat atcgatcgaa tcggcgatga
    11761 tgatcgcgcc gaaacgtttc gagatgcgct caagggtgag gagcgaggac atccggcctt
    11821 tcgctgtcgt cgttatgcca gcggctgcaa tgtgccgccg acggggatat tcggcgcagg
    11881 cttgttggtg gtgatgacaa gatcgagctt gtcgccgctg ccctgccatt ggccagcgac
    11941 cagcggcgtc ttcgtaacgt tcttcaccgg ctggcccgac cacctcaccg gcccgacgat
    12001 cgatacatag tcggtggtcc tgaccgcttc gaggatcgat ttgggatcct caagattctt
    12061 cgtgcgcttg aacacgtcga tcgcaagctc gaacagcgca tgcttgaagc cgatcggctg
    12121 ggtccatggc cgcttggtgg cctgcgtata ggcgtcggtc atctccttcg gagtttgtcc
    12181 cgacaggcca gacttgaacg ggtgcgacgg cgaccaccag acttcggtcg tgaggccgtt
    12241 gccgcgcccc ccgagcgccg caaccgcgga cgggaacagc agcgccttgg cgaccgtcac
    12301 gatcttcggc ttgaatccct gctgcgacgc ctgcgaccag aaggtggcga agtctggcgg
    12361 aatgaacacg ccggtgacga tctcgacatt ggccgccttg aaggcggcaa tctgtgccgt
    12421 gaagtcgttc gacagcggct gatagaagcc cggattgatg agtttgtagc cagcctgcgc
    12481 caatgctggc gggaagccca tattcgggtc ggcaaaggcg ttgccgtcgg cgtcgttgga
    12541 gaacatgccg gcgaccacct tgttggtcgg caggctgtcc cacaacgcga tgaacgcggc
    12601 gagcagatct tccgaacccc agaagaaatg ataggtccag ttgaagccct tcttcgggtc
    12661 gcccttgcgg ccgaaaaaat aggcctgcca cggacagtcc gtggtgatgc acggcacctc
    12721 gttgacttcc gcctggtccg caaccggatt ggtggtgtcg gccgtggagg tcgcgacgag
    12781 aagatcgacc ttgtcgcgca ggatcagttc ggacgcgacc tccgaggcac gcgacgggtt
    12841 cgattgactg tccttggaaa taatctcgat cttgtaggtc ttgccgttgt tctggatgcc
    12901 gttgccgatc agcctctgga tctcgccgag gataaaagga tcggcttcgc cgaaggcggc
    12961 gagcggcccg gagcgcgggc tgacgtgccc gatcctgagc gtgcgcccct gggcattcgc
    13021 cggcagaacc cgcaggatgg cgggagccgc gagcacgccc aatccggtgg tcaatagctt
    13081 gcgccgggac ggcgtggtgt tttgtgtcat ctcatcctcc gttggcttgt tgatggtctt
    13141 cctcgggcgt gcgtctttac cgcgcacgtt gtgtctaaag ctcctcagcg ggcgatattc
    13201 gtgccttttt caagtggtgc gtcacgattg atcggattgc agcttgctgc tccggcggcc
    13261 agcgccggaa tccggccggc tacggcgaac acctgggcga tcccatgccc catcagtccg
    13321 gcgccgacga cagcgatgtt catcggcttg gccatcatca tcccgccgta tagccgccat
    13381 cggcatagag gatgtgcccg gtgtagaagt cggacgcctt cgacgccagg aacaacaacg
    13441 gtccggcgag atccgagggt tcgccgaggc ggcccttcgg cacccgggcg agaaacccct
    13501 tgcgcacgtc cttggcgcgg tcgttgtctt cgaacatcca ctcggtcagc ggcgagcgaa
    13561 acaccgtcgg tgcaatggca ttgaccgtga tgccggtctc gccaagctcg caaccgagcg
    13621 ccttggtcat gccatccacc gcggacttcg atgcacaata ggctgtatag ccggcgggat
    13681 ggcccagcag cccacgggcc gacgacatca gcacgatctt gccaccccct ccctgctcct
    13741 tcatctgttt cgccgctgcg cgcgcaatca gccaagactg ggtgacgtta gcatccatca
    13801 cgtcgagaaa gcgggccggc gacatgtcat tgatcttggc aacgtcgttc ttgccggacg
    13861 cgacgaccag aatatcgacg ccgccaaaac gctcggttgc cttggcgatg atggcaccgc
    13921 aattttcctc ggagctcggg cgcaacagga ccgtctcaac ctctgcgccc agcgcctggc
    13981 aatcggaagc cgacttcgcc agttccttgg cattgcccgc tgcgagcacc agcctggcgc
    14041 ccgcggcggc cagaacctgg gccgcaagcg cgccgaaggc gccggaagcc ccggtgatca
    14101 ccgcgctttt gcccttgacg tcgaacagcg acagcgggtt cttgagccac tccgtcatga
    14161 caccgccttc gatgtcgaca tggcgaccaa catcgtcacc acgtcgttgc cgcggttgat
    14221 gatctcgcgg gcttcgttga tgccgatgaa gcagctgtca ttagccttga ggacggtttc
    14281 cttgccgtcg acgatgaccg tgagttcgcc ggacaggatc acatagacct tttcggtcgg
    14341 tgaggcgtcg ggaccggcgc cgccgccggg caggaagtac gacaacccga cgacaaagcc
    14401 gctggggcca ccggcatcga cgccgaacag gcgcagcgaa gtgtagttgc gatgattggg
    14461 cgcctcatag gccttggctt cgttgaatcg ccggaccaac atcagaatgc ctccccggcc
    14521 tcgatgcgat aagcgccctt gttgtcgatc atggcgacga gacgaatgat acagccatcg
    14581 ccgcgatccc agttccacgg gaagaacgcg aaggtcacgc gcttgcctgt gaccttgtcg
    14641 aggtcgccac cgacgttttc aatgccgaga atgccggcct tgaacagctg gcgatgcacc
    14701 ggctcccact ccggatgatc gtccttccag tcacggccgc cggaccactc gtaatattcc
    14761 ttcttgaggt cgacgtgctg gatgatcggg ccgatgccgt gcggaccaat cgcggtcgcc
    14821 agcggatgat cgttggcctg ggtgtcgtga ccgacgacct tgacctcctt ctcgatgaac
    14881 cagtcggcag ccgaaggcac aaagcccggt gcacgcagga aataatcctc gctgtcttcg
    14941 tagatgtggt gatagccagt gttgacgatc accacgtcgt ggggacgcac cgccttgccg
    15001 gcggcctttt ccaagtcgtc ataggtgatc ggctcccact tcttcttcgg gatcgacacc
    15061 acaatgcccg tcccgaagaa gtgcggcaac ggcacctcgt cgatgaacgg cgtgccctgc
    15121 accacatgcg cgggcgcgtc gatatgggtg gtgttgtgca tgctcgtcgt gatgcgctgt
    15181 gacagcacgc cagacttcgc catgtaatgg atgcgctcga ttttgacgtc ttcgaaatag
    15241 ggccagtttg gcgactgata tccgaaccga tgcgacagat tataaaactc caaccccatt
    15301 tcgttgttgg cgttttcctg aaattcgatg ccgcgaactt tgatcacttg agattcctcc
    15361 tcggtccggc gtcgttattc gcgccttgta ccgcattgtg gtacacagtg tattgtatat
    15421 cggtcggatt tgcaagatgc ccgcggtcgt atgcttctag cgacgccgca ggatcggggc
    15481 agtgcagaat tgggatcaaa ggttttgcga aatgatccgc ccttccagaa cgccctttgt
    15541 ctccagcaac cgccgcgcaa tcgttttgta ttctgccttg aaccgctgac tggggaccgg
    15601 aacggagatt gccacgtaat ttcccgcggg atcgcgcagc gcaactccgg ttgcacagac
    15661 gcccgaggaa tgctcctcga gatcgacggc cacgccggtc tgccgcgccg ttttcagatc
    15721 aacggcgagg tcggcgaatg tcgtcttggt gtgcttggtg cggcgaggat atttccggcc
    15781 gatcagttcg acaatggcgg cgtcgtcgat ttgcgccagg taggccttgc cgttcgcggt
    15841 gcagtacagc ggataggcat cgccgacgac agagacggtc tgcaggcgat ggggtccggt
    15901 gacctgatcg atgaagacaa cctgatcttt cttgatcgtg gacaaatcca cggtttctcc
    15961 aagttccttc gacaacgcga ccagaaatgg ccgcgccaac gaggcgaagt cggtgcggac
    16021 ggacgcggca aggcggagta tggtgggtcc aagtcgcatc tttccgttcg gcgatgcggc
    16081 gatgacgagc ttttcaactt ccagcgcggc gatgatgcgc tgcacggtcg agcgcgcgag
    16141 atcaacgcgt tgtgcgatct gccccagact gaggccggca ttttcattct ccagcgcccg
    16201 caggatcgcg gcagcccgcg caatcacctg gacctggctc ttttcgttgg tgtcgacgat
    16261 cttcacttgg gctccaaaca ttttttgcaa tgcatgtggg ctctcttgtc ggggtgctcg
    16321 cttattgata cactgtgtac cacaatacgg tacaataaga caacccggag gaaatcgatg
    16381 tcgacacctg ttcgagctgg cgtatcgacc agatctcctt tttcctatca ccagtccagg
    16441 ccgccaaggc agctgtcggg ccggcagtga actcacccgc caatcgcgaa agttgtcgtc
    16501 gagcggcggc gtccgggcca aatgaggaga gacctgttgg ttcgcaagat tgcactcgag
    16561 gagcatttcc tttgtccggg cttcgagaaa tattgggcga ccacggttgg cgacgtggcg
    16621 tccgatgtgt tgcagcgcat caccgcgcgg ctgtccgact ttggcgacga tcggatcgag
    16681 gcgatggatc gggccggcgt cgcgcgagcg gtgctgtcgc tggccggccc gggcgtgcag
    16741 agtgaacccg atgccgcagt tgcggtcagg atggccgccg cggccaatga ccatctcgcc
    16801 cgcgagctgc agaagcgtcc ggaccgctac gccgggttcg gccacctcgc gatgcaggat
    16861 cccacagccg ccgccaacga gctggagcgc tgcatgcgcg acctcggttt ctgcggcgcg
    16921 atgattaacg gtcacaccaa cggccaatat ctcgaccacc ccgcgctggc gccgttctgg
    16981 gaacgtgccg cagcgttgag cgccccgatc tacattcatc cggccgatcc ggtgacgccg
    17041 agcccggtgc tcgacggaca cccggcgctg cggcgggcga cctgggaatg gggatttgaa
    17101 accgggtcgc atgccctgcg cctggttttc ggcggcattt tcgcacggtt cccggatgcc
    17161 agagttattc ttggccacct tggcgagacg ctgccctatc tgctgtggcg cttcgacagc
    17221 cgcgccaaac tctacggcgt cgagcttccg cgaccgcctt ccagctacat caagtccaac
    17281 atcgtggtga cgacatcggg catgttttcc gccgagcccc tgaattgcgc gatagcggcg
    17341 ctgggccgcg atcgcgtgat gttctcaggc gactacccgt tcgagtcgct ggaggaggcc
    17401 ggccacttca tggatcatgt cgccctgccc gacgacgttc gcgccgacat cgccttcaac
    17461 aacgcatcaa gcatcctgag cctttagacg atcagcaacc gtccgcagcc ggcaaaagac
    17521 cggaaggact tcaacaatcc ctggaggaaa ccctgacgat gagaatgtcc cgccgaacat
    17581 tcgcgctcac cgctgccggc gcgataatga catctcgctc tgcgtccgcg cagactgccg
    17641 agctgaaggt gtcgcacttc ctgccgccaa atcacacgtt ccagaaagcg atggtggcgt
    17701 ggagcgatga aattgagaaa cagtcggcag gacggctgaa gctgcggatc tatcccgcag
    17761 gccagctcgg cggcgggccc aatcgacaat tcgacgcggc gcgcaacggc gtcgtcgaca
    17821 tcgccatcag cctgcacggc gcgacgccgg gccgctatgc cgtgaccgag ctcgccagtc
    17881 tcccgttcgt ctcgccttcg cccggcaata ccagcgcggt cacttcccgc cggctcagtg
    17941 agcttgcgcc ggaatatctg tcaaaggagc acgagggtat ccgcatcctg tggatggccg
    18001 tcaccccgcc gctgatgttt cacagcaaga cgccgatccg aaccatcgcc gacttcaagg
    18061 gaaagcggat ccgctacgcc ggcgtgcaat tcaagaacat catcgacagc cttggtgcag
    18121 tgccgttgct ggtgccgccg caagaaaccc aggacgggct ggcgaagggc attatcgacg
    18181 cggcaacttt cccctatgag ggcgcggcga gcttcgatat cgcaaccgtt gccaaataca
    18241 cgctggagcc tggcgtcagc gcggcaacct tcgccgtcgt catgaatccg gcgaaatacg
    18301 cctcgcttgc cgacgatctc aaggctctga tcgacaagac aaccgggcct gacgccgcgg
    18361 ccaaattcgg cgcttcgtgg gacgaagccg agcaagaggg aaagacggcg atgctggcga
    18421 agggaacgca ggtatcgacg cttgccaagg accagctcga attgatgaag gccacgttcg
    18481 agcctcaagt tgaaactgcg atcggcgaga tcgagggcca gggcaaaccc ggccgtaaat
    18541 tcttcgaagc ctatctgaag tagcgccgcg aaacggagga accgccaccg gtgccttccg
    18601 gacaaagcca gcccacgacg tatcgaggaa acgccgcgac atgaatcaac aagtatcccc
    18661 gccaccgcag ccgccggact taatccagcg gctgcggacc gcgcagcttc ggcttgctgc
    18721 cgtcgccctg gtgaccatga tgacaggcac cgcgctcgac gtgttcttcc gttacgcgtt
    18781 caacaagcca ttgcggtttt cctacgacct cgtcgagagc atgcttgtga tctacgtctt
    18841 ccatggcatt tcaagtgtct tcctggaacg gcgcaacatc gtcatcgacg tcatcgattc
    18901 gctggtaagc ccgcgcatcc tgcgcttgct cgccttaatc gccgatatcg tttcggtgat
    18961 gtgtctgctg ctgttcctgt ggggaatgct ggagcctgcg atgcaggcgt tccagtatgg
    19021 cgatcgcaag cttgaattgg gcattccgct ctatgtgctc tggttgttcg cgctggcggg
    19081 cctcacgggc acgctcgcct gcgccatcgc cgccgctgcc catatttcgc tgcgccagct
    19141 acggagacaa ttgtgagtcc cggtattgtt ggcgccctcg gcattctgac cctgctggtt
    19201 cttctgttcg ttcgcattcc tgtctgggcg gcgctcgccc tggtcggctt tgccggcaac
    19261 tatattctgt ccgggctgca gagcgcgttg acgctcaccg gaacctccgc tttcgatgtc
    19321 tcatccgcct acaccctctc cgtcattccg ctgttcgtgt tgctcggcga ggtggcctcg
    19381 aatacgcggc tgtcggccga cctcttcaat gcggcgcgcc tgctcctatc cggtgtgcgc
    19441 ggcggccttg cgattgcaac catcgctgcc tccgcctgct ttggcgcggt gtgcggatca
    19501 tcgatcgtca ccgccgcgac catgaccagg atcgcgctgc cggaaatgcg caaggccgga
    19561 tatgacgaca ggctggcgac cggatcgctg gcggccggcg gcagcatcgg aattctgatt
    19621 ccgccgtcga tcatccttgt tatctacgcc tcgatcgccg agcaatcggt tccaaaactc
    19681 ttcgccgcag cgttgtttcc cggcctgatc ctgaccgcgc tctatatggc agtcgcggtc
    19741 ggcatcgcca tcgcctatcc gcattatgcg ccacgcatgg agcgggcatc ggcgcgcgag
    19801 cggctgaagg cgctggcagg cccgtggcaa ttcatcctgc tcttcatcgt caccatcggc
    19861 ggaatctatg ccggcatctt cagcccaacc gaagcggcgg cggttggagc gttcggcagc
    19921 atcctgctcg gagctctcgg tcgccggctg acctgggcga cgctgtggcg ctcgatcgaa
    19981 acatcgatcc ggaccagctg catgctgttc gtcatcatca tcggcgcaaa catcttctca
    20041 tcgttcatgg tgcagaccca gtttcccacc atgcttgcag acggcgccag cgccctcaac
    20101 ctttcggcac cggtcgtgat ggtgatgatc gtgctggcct acatcgtgat ggggtgcttt
    20161 ctcgaaggca tcggcatggt gctgatcacc gtcccggtgt tcctgccgct ggtcgtcaaa
    20221 ttcggctacg atccgatctg gttctccatt ctggtggtca tcgtcgtcga agtcggattg
    20281 atacatccgc cggtcggaat gaaccttttc gtcatccagg cccaggcccc cgacgtaagg
    20341 atcaccagta tctatcgcgg gatcatcccg tttttgatcg cgccgctact gctgatcatc
    20401 attctgttcc tgttcccccg cctcgcgctg tggctgccaa agctgctgta tggatgatcc
    20461 gatgaccaat gtccttgtcc tggaaaccta cgccgaggtc tatcgcgacg cgctgcgcag
    20521 cgaattccac gatctcgaga ttcaggttgc gaactcggcg aacgttgcca ccgtcaattt
    20581 tgcggaggtg gacgtactga tcgcgtttgg catgggcgtc accgatgcgc tattcacgcg
    20641 catgccgggg ctgaagtggg tgcagtcgct ggcgaccggc gtcgaccatt tcctgcgcag
    20701 caagaccctg cccgccggag tgattctcac cagcggccgc ggaatgcacg ggccggcgat
    20761 gcgcgagacc gttctgcatc tgatgctctc ggtcagtcat gacagcaacc gtctggtggc
    20821 cgacaagact gctcgtcgct gggagcggcg accatggccg ctgctggcgg gcaagaccgc
    20881 cgcggtgatc ggcaccggcg tcgccggctc ggcaatcgga gccgcgctgc aggcgctcgg
    20941 catgcgcgtg gttgggatca gcgggacgcc gcgcgacgtg gacggcttcg acgtggtgct
    21001 gccgcgacag gaactgacga aggtcgcggc cgatgcggac tatctcgtca acgtgctgcc
    21061 ggccgatgcc gtaaacctga acctgatcgg gcgcaaggtt tttgccgcga tgaggccgcg
    21121 cgcctatttc atcaatgtcg ggcgcggtga aaccgtcgat gaagcggcct tgatcgaggc
    21181 gctggagcag cgccgcatcg ccggcgcggg cctcgacgtt ttcgccaccg agccgctgcc
    21241 ggccgcaagc ctgctgtggg agatgcccca tgtcttcatc gcgccgcata tcggcggcct
    21301 gttcgaggaa tatgcgcaga tggcgatgcc cctgatcatc cgaaacatgc gcgccttccg
    21361 gtccggtcaa accgacgaga tgatcaacaa ggtggaccgc tcgcgcgctg caacggcgga
    21421 gacccagcca tgagcaacac cgtagatccc gcaagccgga tcctgacccg gatttccgac
    21481 gctgaactga tgcggcgctg ggccgcggtt cgccgcgtca tgcaggagcg cggcgtcgac
    21541 gccatcgtga tgcagaacac caacgactgg ctcggcggct atgtgaaatg gtttaccgat
    21601 cttccggcca acaatggcta cccgcgtact gtcgtcttcc acgccgacgc cccgatgacc
    21661 ctggtcgaga tgggcccctc gaatgtgcgg cggacataca ccgccgacga aacgctgcat
    21721 cgcggtgtcg gcgaaatgct gcatacaccg tcattcacat ccatcgcgta tacggaccag
    21781 gatgatgtgc aactagcgat cgaagccctc cgccgcaacg cgaaacgcgc cattggcctc
    21841 gttacccccg ggacgctgcc ttacgccttt gtctctgcac tgaaggaaca gctccccgcg
    21901 acagcattcg tcgatgtcac tgctgacatc gacgccatca aggcgatcaa gagcgacgag
    21961 gaaatcgcgc tgatccggcg aaccgcccgg gtgcaggacg aggtcttcgc ggaagtcctc
    22021 aaggagattc gtccgggcct gcgagacatc gacgtcacct cgatcgccca acgcaagggc
    22081 cagatcctcg gcagcgaaca gggcatcttc ctcggcggct cgtccccgct cgggatacgc
    22141 tcgcccttcg tcccgcgcta catgcagggc cgaacgctgc aaaggggcga tcacctctcg
    22201 ctgctgatcg agatcaatgg ccctggcggt ttctatacgg aaatcgcgcg cacgatcgtg
    22261 cttggcaaag ctacctcgga gctaacagat gcatttgcgg ccgtccgggc cgcgcagggc
    22321 tattcactga gcatcatgaa gcccggggtt tctgcgaagg agatcttcgc gaaacacaac
    22381 gcctacatga cgaggaacgg catgccggcg gaaacccgcc tctatgcaca tggccaaggc
    22441 tacgacatgg tcgagcgtcc cctgatccgt caggacgatg atatgacgct gcagcaaggc
    22501 atgtgtcttg ccgtgcatcc cggatatgaa accccgtcgc tgttcgcggt gatctgcgac
    22561 aactacttga ttgaggccca cggacctggc gattgtctgc accaaacagc gaaaacgctt
    22621 tttgaaatcg attaacgatc agcccagaac ggccgccacc gcacagggca acaccaaggc
    22681 ctccgccaat gcaatagccc aggtaatcgc tcacaaacct acatgtgtat acaggaatac
    22741 cacattattg gcataaacac gggagctatt gcaaatctaa gccaataatg tatacatatt
    22801 ggatcccact agaggcgtta tcgagaagta tttgtcatga tcccatcgtt cccgatgaat
    22861 gcctggtacg ccgcggcgtg ggatgtcgat atcaaacacg cgctgtttcc tcggacgatc
    22921 tgcggcaagc acatcgtcat gtaccggcaa gccgacggac aagtgaccgc gctggaggat
    22981 gcctgctggc atcggctggt gccgctttcg aaggggcggc tcgaaggcga tagcgtggtg
    23041 tgcggctatc acggcctgaa atacaatgcg tccggccgtt gcaccttcat gccgtcgcag
    23101 gaaaccatca acccgtcggc gtgcgtgcgc gcttatccga tagtcgagcg tcaccggttc
    23161 atctggttgt ggatgggcga tcccgcgata gccgaccccg cgctcgttcc tgacatgcac
    23221 tggaacgacg atccagcctg ggccggagac ggcaagacaa ttcacgtcaa atgcgattac
    23281 cggctggtcg tcgataatct gatggacctg acgcatgaga cgttcgtgca tggcgggagc
    23341 atcggcaatg cagccgtcac ggaagcaccg tttgatgtga ctcacggcga caagaccgtg
    23401 accgtgacgc gctggatgcg cggcatcgaa gcgccgccgt tctgggccaa gcaattgcaa
    23461 aagccgggac tggtcgatcg ctggcagatc attcggttcg aggcgcccgg aaccgtcaat
    23521 atcgatgttg gggtggctcc ggccaacaca ggggcgccgg agggcgaccg ctcgcagggc
    23581 gtcaatggct tcgtgctcaa caccatgacg ccggaaaccg aaacgacctg tcattatttc
    23641 tgggctttcg tgcgcaatca tcatttgacc gaacagaaac tcaccaccga aatccgcgaa
    23701 ggggtcgccg gcattttccg cgaagacgag atcattctcg aagcgcaaca gcgggcgatc
    23761 accgaaaacc cggatcgcgc gttctataat ctcaacatcg atgccggcgc gatgtgggcg
    23821 cgacgcgtga tcgaccgcat ggttgcccgg gaaatcgcac cttcacggat gcacgcggcg
    23881 gagtgagatg tccagagaac gtgatcccga tcgctccgta tctcacaccg tgcgcgccca
    23941 gctcgcgctg cgggatctga tcctttccgg ccggctgcgg ccaggcgagc gaatttcgga
    24001 actgtgggcg gtcgaaacca ccggcgtctc gcgaacaccg gtgcgcatgg cgctggtccg
    24061 gctcgaggag gaaggcctgc tggaagcaat tccttccggc gggttcatgg tgaaggcctt
    24121 caccgaacgt gacatcctcg actccatcga gttgcgcggg gtgcttgaag gtcttgcagc
    24181 cagattcgct tccgagcgag gcgtgtccgc gcgcgatctt gagccgttga aagaggccct
    24241 ggcggaactg gacagactgg tcgcgcagga ttcgatctcg gtggaagcgt tctcgtccta
    24301 cgtcacgatg aacgcccgct ttcatgcact gcttacggaa ttgtctcaca gtggcccgct
    24361 gatccggcaa atcgaccgcg cctccgcgtt accgttcgca tcacccagcg ggttcgtgat
    24421 ggcacaatcg gtgctgccgg aagcgcatca gatcctgatc atcgcgcagg accatcaccg
    24481 cattgtactt gacgcgattg aaaatcgaga aggggcacgg gcggaagcgg tgatgcgcga
    24541 gcatgcgcgc cttgccgctc gaaacttgcg gctagcgctg cgcaatcgaa cgcatctcga
    24601 tctgttgccg gcgttggcgc tgatccgttc tcctgtcgcc gaatgaacgt ttgaggactt
    24661 catgcgattt gaacttaaat ggacgaagtg cacggtggac gcgatacgcg acgtaacgcc
    24721 cgcgattcgt gaaatcgtgc tgcggccgga agggttcggc atcgaaaatt acgctatcgg
    24781 cagtcacgtg aatgttgcgg tgttgatcga cggacggccg gaaacccgct gctattcgct
    24841 ggtcggcgac atcgctcccg atcgataccg gattgccgtt cggcgtgcgc ccgacagtcg
    24901 cggcggctcg ctccatatgt ggtcgctcag gccaggcgcg accatcgagg tatcgaaccc
    24961 ggcttcgctg ttcgaaatcg actggtcgcg aaagtccttt tgcctgatcg cgggcggaat
    25021 cggcattacg ccgatcatcg gcatttcgag tgcgttgacg cgcagaaacg ccgaagtaga
    25081 actgcactac gcggtcaaat cacggcacga tgccgcgttt ctcgacgaac tgtccggttc
    25141 gctgggagat cgtctcacta tccacgccag tgacgaaggc aagcggatca atctggacga
    25201 cactttcggt cgtctgccgg cggatgcgtt ggtgatcata tgcggcccga tgcgccttct
    25261 cgaagcggcc cggcgcagtt gggcggcagc cggacgcgtg ccaaccgact tgcgatacga
    25321 gacatttggc tcgagcggtc ttttgccgac ggaagcgttt cgggtccgga tagataattc
    25381 cggcctcgag atcgtggttt ccgaaagcaa atcgatgctc gccgcactca atgaggctgg
    25441 atacgaagtc atgtcggact gccagcgcgg cgaatgcggt gtctgcgcga tcgatgtcgt
    25501 ttccgtggat ggccagatcg atcatcgcga tgtcttcttt agcgatcaac agaagaagtc
    25561 gaacggcagg atctgtccct gtgtctcgcg tgcggttggc gcaatcaccg tggatacgct
    25621 ttatcggaac gacgctcttt agcgggcgaa ctgcctcctc gccaagccct gcctaatcct
    25681 aggtgatcat gacgttcatt tcactcgttt ggtcgcacca acattcttcg cacgggaaat
    25741 agcgcaatgt ggtagcgttg cgcgacatat ccccaaatcc ccttcggggc gaacaacatg
    25801 atcacaatcg caagtgcacc gagcaaaatc agataccagg tgccaaagtc cgcaaggtag
    25861 tgctgcatcg tgtagaacac catcacgccg atgattggac cttcgatcgt gccgaccccg
    25921 ccgatgatga cgataaaaat cacgtaggcg ctccagtcca gcaccgaaaa cgcggcgtcc
    25981 ggcgagatac gcgccttctg caaatagatc agcgcaccga tcatcccggt cgcggccgcg
    26041 accagcacgt aaacgccgag cttggtgcgg aagctatcga cgccggaagt ttcggcggtc
    26101 gcttcggaat cgcggatggc gaaaagcgca aggccgtagc gcgatcgcaa cacaagatag
    26161 accagcaacg gcgctccagc cgccagcgca agtgcgatcc agtaggtaat aatgtcgcgt
    26221 gcggccggcg agcgcacttc gaacatcacc ttgacccatt cgatgccgaa aatctcgttg
    26281 gtggccgatg gcggcagcga cgtcccagtg cctccaccga gttgcttgaa ttgtgcgaac
    26341 acaagccgat acacctcggc aacgacccaa cttccaatcg caaaataggg accgcgaagg
    26401 cggaatacca caaacgccgt cggcactgcg aatgccgcgg cgatcagccc cgacagcacc
    26461 accgctgcga ttggatcgat atcggcgccg atcgtaagtg caaacagcag atagccgccg
    26521 agaccgacaa atgcctgctg cccgaccgag acgagcccgg cataccccgc aagcatattc
    26581 cagcactgtg ccagcgcgag catatagaag acgaaaatca gatcctgaat cagtccgcgg
    26641 ccggcaaagg ccggcagagc ggcaaagatc aggacaagga cgacgccggc gagcgccaag
    26701 accgaagacg cgcgggtttt cgtttcggta tgatatcgtg tcggctccat caattggttc
    26761 ccgtgacccc tcgccatcac cgttcaatcc accgcgcgag gaaacaggcc gcgtggccgc
    26821 agcaccacca cgaacagaaa ggcgatgtga cccgccaata tctgccattc cgggttcagc
    26881 ttggcgccga cggtctgcgc caggccgata atgatgccac ccgcgagcgt gccccacaga
    26941 ctcccgaggc cgccgatgat cacggcctcg aacgcgtaga tcagccgcgc cggaccgatg
    27001 gtcggatcga aattggcgcg gatgccaaga tacagcgctg caatcgtcac caccaccatc
    27061 gcgatcccca tggcgatcga gtagattcga tgatggtcga tgcccatcaa ctgcgcgatt
    27121 tcgagatcgt cggaggtggc gcgaaatgcc cgcccgagcg cagtgcgata aaacaacaga
    27181 ttgagcagca ggatcaccgc gatcgcagag ccaagcgtaa gcagcggcat cacgccgatc
    27241 gccagattgc cgccgagatg aattgaagcg gtctcgatcg cgccgagccg cagccggcgg
    27301 ctgtcggcgg taaaggcctc gagcagcccg ttttgaatga tgatggacag cgcgaaggtc
    27361 accagcagcg gcggcaaaag atcacgtccc aacgttctgt tcagcagcac atgctgcaag
    27421 gcaaaacccg cggcgaacat cgccggcatt gcgagcagag ccgcgagaaa tggatcgaag
    27481 ccgaaccacg tcgccagcgc gaggatcacg aacgccgcca gcacgatcag atcgccatgg
    27541 gcgagattga ccagccgcat gatgccgaag atcagcgaca ggccggcggc gaacagcgca
    27601 tacaggccgc caagcagaac gccctggatg atcgtgtcga gccaatcgag catgctcaac
    27661 gtccgaaata ggcaaggtga acctgttcgc gtgttagttc cgttggccgc cctgacagcg
    27721 acaccctacc ctcctggaaa
//
LOCUS       DASWGM010000393         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2661592,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000393 DASWGM010000000
VERSION     DASWGM010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661592"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1356)
                     /locus_tag="VGA15_11530"
     CDS             complement(211..1356)
                     /locus_tag="VGA15_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588359.1"
                     /translation="MKLTRRDFTAGLAVGISAPYVIGSARAQAATIKIGMCVPVTGPA
                     AEQGLWAQNGAKLALAAVNKAGGVLGKQVELVIEDDQTTNPGIVLAFSKLAAQPDIVA
                     FLGSIRSTQVMAMAPDVLKLGKPVMIGGTNPDLTHMGNQWLFRFRPNDSYSGRVIADY
                     GVNTLGKKKWAIVHSTDAFGTAGGKALSDALGKLGAPPVLDQGYANQSQDFTPVVLAV
                     KQSGADVLGTYFTFENDLGIFARQLRQLGVNIPWVGSPSVVAVSSTKLAGPALFGTYG
                     VADYAEDSSPASKAFGTAYREAYKTAPDNQSAWPYDAVTILSAAINKAGSTDPTKIRE
                     AILAVKKYAGAEGEYNFDQNGDGLHGYNVVRNEKGTIVYDKHIDFND"
     gene            complement(1552..2016)
                     /gene="hpaR"
                     /locus_tag="VGA15_11535"
     CDS             complement(1552..2016)
                     /gene="hpaR"
                     /locus_tag="VGA15_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HEX9588360.1"
                     /translation="MREFSRSLPMSLLRAREAVMRHFRPSLRDHGLTEQQWRILRALA
                     SIDAIEVTELARVAFLLGPSLSRILRDLEARHLIERRAAKADLRRGVVSISAKGLKLI
                     EAVAPSSEAIYAAITRRYGVRKLAELQDMLHALEGSLSGLQVVGGNAGAEFE"
BASE COUNT          437 a          695 c          654 g          463 t
ORIGIN      
        1 atgcctatac cggtgaagag caattgcagt acgagatcca tggaaacgct cggttactgg
       61 aagctgatcg gctctgctgt tggtgattgt tgctgttgcc gcaggccaaa atcggggtgg
      121 atcgatcagc gcaacgcaag ggctgcgcag ataatgactg acgcgatgcg catcggcccc
      181 tccccatcgg gaggggccag caacgcgggg tcaatcgttg aagtcgatat gcttgtcgta
      241 aacgatcgtc cccttctcgt tcctcacgac gttatagccg tgcagaccgt cgccgttctg
      301 gtcaaaatta tactcgcctt ccgcaccggc atattttttc acggcgagaa tggcctcacg
      361 gatttttgtc gggtcggtcg agccggcctt gttgatcgcc gccgacagga tggtgacggc
      421 atcgtagggc caggcgctct gattgtccgg cgcggtcttg taggcttcgc gataggcggt
      481 gccgaaagcc ttcgacgccg ggctggaatc ttccgcgtaa tccgccacgc cataagtgcc
      541 gaacagcgcc ggacccgcca gcttggtcga tgaaaccgcc accaccgacg gcgatcccac
      601 ccagggaatg ttgacgccga gctggcgcag ctggcgcgcg aaaatgccga ggtcgttctc
      661 gaacgtaaag tacgtgccga gaacatcagc tcccgattgc ttcacggcca gcacgaccgg
      721 cgtgaaatcc tggctctggt tggcataacc ctggtcgagc accggcggcg cgccaagctt
      781 gcccaatgca tccgacagcg ccttgccgcc ggcggtgccg aacgcgtcgg tcgaatgcac
      841 gatcgcccat tttttcttgc cgagcgtatt gacgccgtag tcggcgatca cgcggccgga
      901 atagctgtca ttggggcgga aacggaacag ccactggttg cccatatggg tcaggtccgg
      961 attggtaccg ccgatcatca caggcttgcc gagcttgagc acgtcgggcg ccatcgccat
     1021 cacctgggtc gatcggatcg acccgagaaa cgccacgata tccggctgcg cggccagttt
     1081 cgaaaacgcc agcacgatgc ctggattggt ggtctggtca tcttcgatga cgagttcgac
     1141 ttgcttgccg aggacgccac cggccttgtt caccgcagcg agcgcgagct tcgcaccgtt
     1201 ctgcgcccaa agcccctgct ccgccgccgg acccgtcacc ggtacgcaca ttccgatctt
     1261 gatggtcgcg gcttgcgcgc gggcgctgcc gataacgtaa ggtgcgctaa taccaacggc
     1321 caacccggcc gtaaagtcgc gtcgcgtaag cttcatccat tcctcctgtg aagccaactt
     1381 gagtgctggc cttcttaagt ttctgacttt gagcgaaaaa tcgctcgcgt gtctatgccg
     1441 agcgtcagaa agagtaaatt ggcctggcca tcaattccgc gttgcacacg cctgctcaaa
     1501 caatgagcat ccagccatgc ggtctattca cgccgtcatg caaatttgcg ctcactcgaa
     1561 ttcggccccg gcatttccgc caacaacttg caaccccgac aggctgccct cgagcgcatg
     1621 cagcatatcc tgcaactcag cgagtttgcg cacgccataa cgcctggtga tcgccgcata
     1681 gatggcttcc gatgacggcg cgaccgcttc gatcaatttc agaccctttg cggaaatcga
     1741 tacgacgccg cggcgcagat cggctttcgc ggcccgtcgt tcgatcagat gacgcgcttc
     1801 caggtcgcgc aggatccggg aaagacttgg ccccagcaga aaagcaaccc gcgccagttc
     1861 cgtgacctcg atggcgtcga tcgaggcgag cgcgcgcaag attcgccatt gttgttcggt
     1921 cagaccgtga tcgcgcaacg agggacggaa atgccgcatc accgcttcac gcgcgcgcag
     1981 cagcgacatc ggcaacgaac gggagaattc ccgcatcggc acgcgccgga tgccggcctt
     2041 gctcggcggt ttcgaacggg cagttctgcg cggttttttt tgtggcaatg gtttgcgctg
     2101 caacaacgat tgttccgttt gatcggtttc acttaacatg ttaatcagat ttcagcaccc
     2161 ctttttgctg atcccgcgta tgtgggctgg accgtgcatg gccctgacaa agacgatatc
     2221 cgtcgcgccg ccgagcggct tcatcggcg
//
LOCUS       DASWGM010000394         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2537168,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000394 DASWGM010000000
VERSION     DASWGM010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537168"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1452)
                     /locus_tag="VGA15_11540"
     CDS             complement(175..1452)
                     /locus_tag="VGA15_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX9588361.1"
                     /translation="MNTSTMMTTAMPSAPVGKAWEEVSASFDRFCLAAGIETLGAMME
                     QDAEVACGPRHSRGEERRGHRWGRTVGKIGFHAGKVAIERPRVRGLNGKEHSLASWEQ
                     AVGEDWLGQWAMNLMLINVSTRKFRRAVRLPEGDVPAPAGSGVSKSAASRHFVALSAA
                     RMKEWMTSDLSKLDLLVVQIDGIHMTGELVLVAAIGIDGDGVKHPLGLIEGATENAAV
                     VQALIDNLVARGLDPAVPRLFIIDGSKALSKAIRHTFGREALIQRCQIHKARNIMERL
                     PKPLHAAVRRVLRQAWELKDAGKAEKLIRNLARRLEHDAPGVSASILEGLDEILTVTR
                     LGLPKELRRALACTNIVENVMSTVRRVCRNVKRWRSASMALRWTAAAMQEAAKGFRRL
                     KAHKQLPALRAALEEHRRNKSTHGVLARQANAA"
BASE COUNT          282 a          560 c          489 g          310 t
ORIGIN      
        1 acgatcccgg agctggtcgc acaagccaag accgatccgc tcaattatac ctcgcccggc
       61 ccgcgcggcg tcggccatct ggcgggcgag atgttgaaac agcgggccgg gatcgacatg
      121 aagatgtccc gctgattgtt gaacattgcg aagcggacgc tgccgagatt gacgttacgc
      181 ggcgttggct tggcgagcaa ggacgccgtg ggttgacttg tttctgcgat gttcttccag
      241 agcggcacgg agcgctggaa gttgcttgtg agctttcagg cgtcggaagc ctttggcggc
      301 ttcctgcata gcagccgcgg tccagcgcaa tgccatcgaa gccgatcgcc atcgcttcac
      361 gttgcggcag acgcgacgca cggtgctcat gacattctcc acgatgttgg tgcaggcgag
      421 cgcccggcga agttctttcg gcaggccgag ccgcgtgacg gtgaggattt cgtcgaggcc
      481 ctccagaatg ctcgccgaca cgcccggcgc gtcgtgttcc agccgccggg cgagattgcg
      541 gatcagcttc tcggccttgc cggcgtcctt caactcccag gcttgacgca gcacccggcg
      601 cacagcagcg tgcaacggct tcggcagccg ctccatgatg ttgcgcgcct tgtgtatctg
      661 gcaccgttga atgagcgcct cgcgcccgaa ggtgtgccgg atcgctttcg agagcgcctt
      721 cgatccatcg atgatgaaca accgcggcac ggccggatcg agcccgcgcg cgacaagatt
      781 gtcgatcagg gcctgcacga cggcggcgtt ctcggtcgcg ccctcgatca gcccgagcgg
      841 atgcttcacg ccgtcaccgt cgatgccaat cgccgccacc aacacgagtt cgccggtcat
      901 gtggatgccg tcgatctgca ccaccaggag gtccagcttg gacaggtccg aggtcatcca
      961 ctccttcatg cgtgcggccg agagcgctac aaagtggcgc gaggccgccg atttcgacac
     1021 cccactgcct gccggcgccg gaacgtctcc ctccggcaat cggacagccc gacggaactt
     1081 ccgcgtcgat acgttgatca gcatcaggtt cattgcccac tggccgagcc aatcctcgcc
     1141 gaccgcctgc tcccagctcg ccagcgagtg ttccttgccg ttaagcccgc gcacccgagg
     1201 gcgctcgatc gccaccttgc cagcgtgaaa gccgatcttg cctaccgtgc gcccccagcg
     1261 atgaccgcgc cgttcctcgc cgcgcgaatg ccgtggccca cacgccactt cggcgtcctg
     1321 ttccatcatc gcgccgagcg tctcgatccc ggccgccagg cagaaccgat cgaaggacgc
     1381 agacacttct tcccacgcct tacccacagg cgcgctcggc atcgcagtcg tcatcatggt
     1441 agatgtgttc atggcgttgc tttcctttcg tggattcgac accccgagcc taccggctca
     1501 aggcgagcaa cgccgctcct tctatttcaa catcgaccgg gacatttccg cggtaggtcc
     1561 gtcggcaact ctgtactttc gttgcgccgg cggctgatca aaatcgactt cgccaaggcc
     1621 gttccaaaga ccgagcggca g
//
LOCUS       DASWGM010000395         9436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2260234,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000395 DASWGM010000000
VERSION     DASWGM010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260234"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VGA15_11545"
     CDS             <1..330
                     /locus_tag="VGA15_11545"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX9588362.1"
                     /translation="ARLAGLEAPTVAEIMRPKIREFYSVGDKIGPWPIFFIGDNEIVA
                     GRNNKHMDFRLSVLKAMDGDATSVVVSTICMVHNVFGKIYLFFIIPFHRNGVQSLMSN
                     AVVAKRL"
     gene            493..1698
                     /locus_tag="VGA15_11550"
     CDS             493..1698
                     /locus_tag="VGA15_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588363.1"
                     /translation="MRFVTRFSVASALVVLTWPAATPAAEMRGVTATEIKIGQTMPYS
                     GPVSAFGALGKGEVGYFKMVNERGGINGRKINLISLDDSYAPPKTVEQTRRLVESDEV
                     ALIFSSIGTAHNTAIAKYLQGKNIPQLFVGSGASKFADIAQYPQATLGVQGPFRYEAR
                     LYARHALAKNPNAKFAVISQNDDFGRDYLLGLKDVLGEKYDSVVTGATYEISEPTIDS
                     QIVKLKASGADVLVIAATPKFAAQSIRKVFEIGWKPMTFLSNVAVWVSTVMEPAGLQA
                     GVGILSTAYVKDPDDPAWKDDAGVKGWREFMIKYVPDADLHDTNYVNAYNSAMALEAV
                     LKACGDELSTENILKQAYAIEDLELPMLLPGIKVNTSPTDHIPVEQMQFMRFNGKTWE
                     RFGELQTGN"
     gene            1931..2089
                     /locus_tag="VGA15_11555"
                     /pseudo
     CDS             1931..2089
                     /locus_tag="VGA15_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
     gene            2610..3722
                     /locus_tag="VGA15_11560"
     CDS             2610..3722
                     /locus_tag="VGA15_11560"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX9588364.1"
                     /translation="MTSIATIQSPRHPTSAMKLVDRWLLLGSYGPAARLHMASVWITL
                     SAFLVVDTDWLWYSRLSFASANLDCVIRLVLFTAIAFGFCGLVSHRLAHETDRVGRAL
                     REYVRRVELFAVAALVFALLAVAVIVFCGLGTSAALPLQDARLAQIDQWLGFNWVAFV
                     EVANSSALASWLLVKAYQSTAAVLVGTILWFCISGQGNRLAEFLALLCVTSIGIAIGM
                     MILPAAGAYSYYHPPLASYENFGVGAGMWHYDLLMALRTGSTSVIDFSVPNSNCLVTF
                     PSGHTILAIIMTYALRTSRYTLIPALLINGTMLVSTIPEGGHHLFDLIVGGVIAALAI
                     SFVRLPQGVRCCRLAASGEFGLANGAPNSCRNGGKP"
     gene            3805..5025
                     /locus_tag="VGA15_11565"
     CDS             3805..5025
                     /locus_tag="VGA15_11565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9588365.1"
                     /translation="MTRPVSAPTITEPGAIPASLLNLCFVLFVINAVFFPTAFFAHWW
                     IFDENGLGIPTDFVNVWSAGKLVLDGHPAQAYDWDIQKQLQVAVLGQSYQGNFAWHYP
                     PPFLFVASLLAHFPYAVAFIGWAAVSLVPYLAVMRGIVGRPFGLLLALAFPVVLTNTL
                     VGQNGFLTASLIGGTLYLMPARPVLSGICLGLLSYKPQYGLLFPLVLIAASQWTVFFT
                     AGAVAVAMALASWLAFGTESWQAFFHWMPTFSQAFLTEGRAPWGKMQSIFAVVRYFGG
                     TEPLGWLFQWIMSATVAVVLTLVWRGRISYPLKAAALATGTLLITPYLFLYDLMVLAI
                     AMAFLVRIGLRKGFERYELPALGLAAALLMFYPLVGAPTGFVATLIVTVLIARRAGPW
                     WRREQAPSLMAASA"
     gene            complement(5167..6660)
                     /locus_tag="VGA15_11570"
     CDS             complement(5167..6660)
                     /locus_tag="VGA15_11570"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose transferase"
                     /protein_id="HEX9588366.1"
                     /translation="MTETSAAPLNGRASRLTIPIVRFHSLAGLLLLAAAIRLPLAFWP
                     NVLHPDEIFQCLEPAWRMLGHDGIVSWEWRYGMRGWLLPSLMAGPVGLGDWVAPGGTG
                     AFVVPRLVAALASLSIVASAWSLGARVSRTHAIVAAFVAAIWFELVSFAPHRLSEPLA
                     TAVILPAALLLTHASLSQRHLAVGGALLALAFVFRFQYAPAIAVLALGACWRHWRNLI
                     PLVAGASIGLVFGGVADAAHGAVPFAWLIANVQQNLLHDRAAEFGVTPAITYLYSFWM
                     MWSVALVLLLFAIWQGWRHAPLLLATAVANIAVHSLIGHKEYRFIFLSVALFIIIAAL
                     GSADWIQWLRANRAWRRWALPVVAGGVGADLGGACGDRYDAGQLDARHGCGATGGRIT
                     RRSGALRSRALQHAFPPASGAGPIGRPVAALRAPPCRSGRKRRPRGRSKESRAGVQPH
                     PLATGLGNGSSGGLFTTRLRQRRRSRCVYLRAKRRLRRGDRGAICPQ"
     gene            7159..7833
                     /locus_tag="VGA15_11575"
     CDS             7159..7833
                     /locus_tag="VGA15_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9588367.1"
                     /translation="MVYRRTHQVVKRLAARRSAILAAARDAAADGGMAAVQIAPVAIR
                     ANVAAGTVYRYFPSKADLISELIADVSRDELAAIRRAADAAPGPSSALAAAVTTVAVH
                     VLSQRKLAWGILAEPVDVDVTASRLESRREIAGEIAARIEAAVRAGHLPAQDTAVAAT
                     ALLGALHESLVGPLAPDHSEDPARLRDAVQTVTLLALRAVGVMDARARGLVVQAVLPA
                     KTLVGA"
     gene            7938..8363
                     /locus_tag="VGA15_11580"
     CDS             7938..8363
                     /locus_tag="VGA15_11580"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9588368.1"
                     /translation="MTTTFGSAKWQGGIKDGKGAISTKSGALKDYPYGFASRFEGKPG
                     SNPEELIGAAHAACFTMALSLILGEAKLTAEQMETKADVTLEKVGDGFAITSVHLALT
                     AKIPGADKAKFEELAGMAKAGCPVSKLLNTKITLDATLL"
     gene            complement(8660..>9436)
                     /locus_tag="VGA15_11585"
     CDS             complement(8660..>9436)
                     /locus_tag="VGA15_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9588369.1"
                     /translation="PQAIDRLPEIIEVSDALMVARGDLGVELPLERVPSLQKQMTRLA
                     RRAGKPVVVATQMLESMIQSPVPTRAEVSDVATAVYEGADAIMLSAESAAGKFPVEAV
                     STMNRIGEEVERDPTYRGVLTAQRAEPEPTVGDAIADAARQIAETLDLSAIICWTSSG
                     STALRVARERPKPPVVAITPNLATGRKLSAVWGVHCVVAEDAKDLDDMVNRAGSIAFR
                     DGFARAGQRVIIVAGVPLRAPGTTNMLRIASVGPNDDANM"
BASE COUNT         1735 a         2946 c         2927 g         1828 t
ORIGIN      
        1 gccaggcttg ccggtttgga ggctccaacg gtcgccgaaa tcatgaggcc caagatcaga
       61 gagttttaca gcgttggcga caagatcggc ccttggccga ttttcttcat tggcgataat
      121 gaaattgtcg cgggtcgtaa caacaagcat atggacttca ggctgtcagt actgaaggcg
      181 atggacggcg atgcgacgag cgtcgtcgtc tcgacgatct gtatggtgca taacgtcttt
      241 ggcaaaatct atctgttctt catcattccc ttccatcgga acggcgttca gtcgttgatg
      301 tcgaacgccg tcgtggcgaa gcgcttatga gtcttgccgt gttcgatagc cgtcattttg
      361 cgtctgaaat ataactatta tggttggcgt ccatcccggc gtcatggcat gttcccttac
      421 gacaagagcc cgataaatcg cgccagtcgt ggtcggccct ttgggcggtc caaaaaaggg
      481 gagggaacaa cgatgcgttt cgtcacgaga tttagcgtag cgagcgcgct tgttgtgctg
      541 acgtggcctg cggccacgcc ggccgccgaa atgcgtggcg tcacagcaac cgaaatcaag
      601 atcggccaga ccatgcctta cagcgggccg gtgtcggcgt tcggcgcgct cggcaagggc
      661 gaggtcggct atttcaagat ggtcaatgag cgcggtggca tcaacggccg taagatcaac
      721 ctgatttcgc tggatgacag ttacgcgccg ccgaagaccg tggagcagac gaggcggctg
      781 gtggagagcg acgaggtcgc gctgatcttt tcctcgatcg gcactgccca caacaccgcg
      841 atcgccaaat acctgcaggg caagaacatc ccgcagctgt tcgtcggctc tggcgcttcg
      901 aaattcgccg acatcgcgca atatccgcag gcgacactgg gcgttcaggg cccgttccgc
      961 tacgaggcgc ggctttacgc gcgccatgcg ctggccaaga atcccaatgc caaattcgcg
     1021 gtgatttcgc agaacgacga tttcggccgc gactatttgc tgggcttgaa ggatgtgctc
     1081 ggcgaaaaat acgattctgt cgtcaccggc gcgacctacg agatctccga gccgaccatc
     1141 gactcgcaga tcgtcaagct gaaagcctcc ggcgccgatg tgctggtgat cgcggcgacg
     1201 ccgaaattcg ccgcgcagtc gatccgcaag gtctttgaga tcggctggaa gccgatgacg
     1261 tttctgtcca acgtcgcggt ctgggtgtcc accgtgatgg agcctgcggg gctgcaagcc
     1321 ggcgtcggca ttttgtccac cgcctatgtc aaggaccccg acgatcccgc atggaaggac
     1381 gatgccggcg tcaagggctg gcgggaattc atgatcaaat acgttcccga cgccgatctg
     1441 cacgacacca actacgtcaa cgcctacaac agtgcgatgg cgctggaagc ggtgctgaaa
     1501 gcctgtggcg acgaactctc gaccgagaat attttgaagc aggcctacgc catcgaggac
     1561 ctggaactgc cgatgctgct gcctggcatc aaggtcaaca cctcaccgac cgatcacatt
     1621 ccggtcgagc agatgcagtt catgcgtttc aacggcaaga cctgggagcg ttttggcgaa
     1681 ctgcagaccg ggaattaatt catgcccggt cattcccggg cacgcgtctt cgcgcgaacc
     1741 tcagatgtgc aattgcacat cggggaatct caagattcca ggttcgccgc ggagcctgtc
     1801 agcgggccgc gccttgcgcg gacccgttgg cgtcgccccg gaatgacaac gataggatct
     1861 cgtcgcgcag ccaccctttt tggcgcggga cttgcttcga gagacggcca gttgagcgta
     1921 ggagccgccg atgtcaggcc actcgaatgc gccacccgct attgccatcg cgggcgcgga
     1981 tccccggctc tataaccacg atctggcgcc ggtcgcgcct gcggggcgaa cctggggcgt
     2041 gttcagcatc ttcgcgatgt ggatgtcgga tgtgcactcg gtcggcggcg atacgttcgc
     2101 ggcctgatcg gtgccacgct gggcgctgtg ctgtacacgg cgctcatgcg ccgcgaagcg
     2161 ccgtcgctgt cgcggtccgg cggcgcggtg gcgtaattcg tcgacgttcc ggagcgcatc
     2221 gagcgggtcg acgcaaagct tggcccgatg gcgagcccga acccgggatt tcaagcttcc
     2281 ggctctcgcg ccgcgcccga tgagcgcgcg agcttccgaa tcccgaaggt tccgataatt
     2341 ttcagatggg caatcgcaca accggggtcc gttcgttcgg gccagcggga acggcgaatg
     2401 tcttggccca actgagcgcg gcccgaacca gcgaccggcg tttcgaacca agattctcat
     2461 gctcgggtcg ccgccacgat gccgcgaagc attgcaattt aaacaacgga ctaacaaacc
     2521 cttaaaattg cctccgtagt tatacggtgc agccacatgg gcgccgcgcc ctcttccgat
     2581 ccccgaataa ccttgaacct ccggcaaaca tgacatcgat tgcgaccatt caatctcccc
     2641 gtcatcccac ctcggcgatg aagcttgtcg accgctggct gttgttgggg agctatgggc
     2701 ccgccgcgcg tcttcatatg gcgtcggtgt ggattaccct ttccgccttt ctggtcgtcg
     2761 atacggattg gctttggtat tcccgattga gtttcgccag cgctaacctg gactgcgtta
     2821 ttcggctcgt tctattcact gccatcgcgt tcggtttctg cgggctggtt tcgcatcggc
     2881 tggctcacga gaccgaccgc gtcggccgag ccctgcgcga atatgtcagg cgcgttgagc
     2941 tatttgccgt cgcggcgctg gtttttgcac tgcttgcagt tgccgtcatc gtgttctgcg
     3001 gcctcggcac cagcgccgcg cttccgcttc aggatgcccg gctcgcccag atcgaccaat
     3061 ggctcggctt caattgggtt gcctttgtgg aggtcgccaa ctccagcgcg ctggcaagct
     3121 ggcttctggt gaaagcctat caaagcacgg ctgccgtact ggtgggcacc atcctgtggt
     3181 tttgcatttc ggggcagggc aaccgtctcg cggaatttct ggcgctgctg tgcgtgacat
     3241 ccatcggcat cgccataggc atgatgatcc tgcccgcggc cggcgcttat tcatattacc
     3301 atccgccgtt ggccagctac gaaaatttcg gcgtgggcgc gggaatgtgg cactacgatc
     3361 tgttaatggc gcttcgaacc ggctccacgt cggtgattga tttttccgtg cccaattcca
     3421 attgcctggt cacgttcccc tcggggcata cgatacttgc gatcatcatg acttacgcac
     3481 tgcgcacctc acgatatacc ttgatcccgg cgctattgat caacgggaca atgttggtgt
     3541 ccacgatccc ggagggtggc catcacctgt tcgacctgat cgtcggcggc gtgatagcgg
     3601 ctctggccat ttcattcgtc cggctgccgc agggtgtccg gtgttgtcgt ttggcggcca
     3661 gtggcgaatt cggcctggcc aacggcgccc cgaattcttg ccggaacggc ggcaagcctt
     3721 aagatttcct aagggctgtt tgtcctattg ctaggtcggg tgcgcgttcc gccgaggcgg
     3781 cgagcgcgcg caaagggact tcgcatgacc aggcccgtat ccgccccgac gatcaccgaa
     3841 cccggcgcaa tccctgcgtc gctgctgaac ctgtgcttcg ttctgttcgt catcaatgcc
     3901 gtatttttcc cgaccgcgtt cttcgcccat tggtggattt ttgatgaaaa cggcctgggc
     3961 atcccgaccg atttcgtcaa cgtctggtcg gccggaaagc tggtgctcga tggtcacccc
     4021 gcgcaagcct acgattggga tatccagaag cagcttcagg tcgcggtgct cggccagagc
     4081 tatcagggca acttcgcctg gcattatccg ccgccgttct tgttcgtcgc cagcctgctg
     4141 gcgcattttc cctacgcggt agcctttatc ggctgggcgg cggtcagcct cgtgccctat
     4201 ctcgccgtca tgcgcgggat tgtcggccgg ccgttcggct tgttgctggc gctggcgttt
     4261 ccggtggtgc tcaccaacac gctggtgggg cagaatggtt tcctcacggc gtccctgatc
     4321 ggtggcacgc tctatctgat gccggcgcgg ccggtgctat cagggatctg tctcgggcta
     4381 ttgagctaca aaccgcaata cggattgctg tttccgctgg tgctgatcgc ggcctcgcaa
     4441 tggaccgtgt tcttcaccgc gggcgctgtg gcggtcgcga tggcattggc atcatggctc
     4501 gccttcggca ccgagagctg gcaggcgttt tttcactgga tgccgacgtt ttcgcaggcc
     4561 ttcctcaccg agggccgcgc gccctggggc aagatgcaaa gcattttcgc ggtggtgcgg
     4621 tatttcggcg gcaccgagcc gctcggctgg ctgttccaat ggatcatgag cgccaccgtc
     4681 gcggtcgtgc tgacgctggt gtggcgcggc cgcataagct acccgttgaa ggcagccgca
     4741 ctcgcgaccg ggacattact gatcacgcca tacctgtttc tctacgacct gatggtgctg
     4801 gcgattgcga tggctttcct ggtccggatc ggattgagga agggctttga gcgttatgaa
     4861 ttgccggcgc ttggcctcgc cgccgcactt ctcatgttct atccgctggt gggggcgccg
     4921 accggcttcg tggcaacgct gatcgtgacg gtgctgattg cgcgccgcgc cggtccctgg
     4981 tggcggcgtg agcaggcgcc gtcgttgatg gccgcgagcg cctagcgccc gccaatcctg
     5041 accgcggtgg attatatctg ccatgcaatg tggtgtgccg cgccgcgttc actccctgca
     5101 attggcgcta gtcgcctagg cagccgctac gctagcgagc cctatcggtc aatgcgcacc
     5161 agcacgtcat tgaggacaaa tggcgccgcg gtcgccgcgt cgcagccgcc gcttcgcgcg
     5221 aagatacaca catcggctcc gccgacgctg gcgcaatcgc gttgtgaaaa gcccgccgga
     5281 agatccgttg ccgagtcccg tcgcgagagg atgcggttga acgccggcgc ggctttcttt
     5341 gctacggccg cgaggtcgcc gcttgcgacc ggatcggcag ggtggagcgc gtagagcggc
     5401 gaccggccga ccaatcggtc ccgccccgga agcaggtgga aaggcgtgtt gtaaagcgcg
     5461 agaccgcaga gcgccggatc gccgcgtaat tcggccgcca gttgcgccgc acccgtgccg
     5521 cgcatccagt tgtccggcat cgtaccggtc gccgcaagcg cccccgagat cagcgcccac
     5581 ccccccagcg accaccggca gcgcccatcg ccgccaggcg cggttcgcac gcaaccactg
     5641 gatccaatcc gccgagccga gcgccgctat gatgatgaac agggccactg acaggaatat
     5701 gaagcggtac tccttgtggc cgatcaggct gtgaacggcg atattcgcga ctgccgtcgc
     5761 caacaacagc ggcgcgtgcc gccatccctg ccagatcgcg aacaacagca gcacgagcgc
     5821 gactgaccac atcatccaaa aactgtagag ataggttatc gcgggcgtca cgccgaactc
     5881 ggccgcgcga tcatgcagca gattctgttg tacgttcgcg atcagccagg cgaaaggcac
     5941 cgcgccgtgc gcagcatcgg ccacgccacc gaaaaccagc ccgatagatg cgccagcgac
     6001 cagcggaatc aggttgcgcc aatgcctcca acatgcgcca agcgcgagca cggcaatggc
     6061 tggcgcatat tgaaagcgaa aaacaaaggc gagagcaagc aaggcaccgc cgacggcgag
     6121 atgtctttgc gacaaggagg catgggtcaa cagcagcgcc gccggcaaaa tgaccgcagt
     6181 cgccagcggt tcgctcaacc tatgcggcgc aaaggaaacc aattcgaacc agattgcagc
     6241 cacgaaggcc gcaacgatcg catgggttcg cgagacccgg gcgcccaaag accatgcgct
     6301 ggcgacgatc gacagcgacg ccagcgccgc caccagacga ggcaccacaa aggcgccagt
     6361 gccgcccggc gcaacccagt cgcccaagcc taccggcccc gccatcaagc tcggtaacaa
     6421 ccagccacgc ataccgtagc gccattccca agagacgatg ccgtcgtggc cgagcattcg
     6481 ccaagccggc tcgaggcact ggaagatctc gtcgggatga aggacgttcg gccagaacgc
     6541 cagcggaaga cggattgctg ccgccaacaa cagcaaccca gccagcgaat gaaaccgcac
     6601 gatcgggatt gttagtctgc tcgccctgcc gttcaacgga gcggcgctcg tttccgtcat
     6661 gttccgccga ccatcgcgcg catagctgcg gcatcgcgtg gagccgctta ttcgaaacga
     6721 attataccgc cgaagccgtt aaagacgcgt gactggagca gaggttacac gttcaccgaa
     6781 cctgcacggc gtcagcacac ccgtcgccaa cgatctcgta gttcctaagt ggcggtcgcg
     6841 gcatgtgaat gatcgacgcg ttggaagccg tgaatgcgct tatcctcaaa aaggccgatt
     6901 atggtttccc gaccgccgga tggcatccat ctcgatgccg ccggctatgc aatctgagat
     6961 aaggccgcct tgggcggcat cgagtccgct ctctgcaaat cctcctgaaa cggtcgtgaa
     7021 ccatcttgtc aggcggcctg aataggtgtt cagttctttg aagcgaattg cgaaaaattc
     7081 gcccaaaaca ctgaagatgc gcgttctcga tcggctctca accggtgtcg gaatgcgccc
     7141 agggacgaga aacacgccat ggtttaccgg cggacccatc aggtggtgaa gcggcttgcg
     7201 gcgcgccgta gcgccattct ggcggcggcg cgggatgccg ccgctgacgg cggcatggcc
     7261 gcggtgcaaa tcgcgccggt cgcgatccgc gccaatgtcg ccgccggcac cgtataccgc
     7321 tattttccct ccaaggcgga tctgatttcc gagctgatcg ccgatgtctc gcgcgatgaa
     7381 ctggccgcga tccgccgcgc ggcggacgct gcccccgggc catcctcggc gctggcggca
     7441 gccgtcacca ccgtcgccgt gcacgtgctg tcgcagcgca agctcgcatg gggcatcctg
     7501 gccgagccgg tcgatgtcga tgtcaccgcg tcgcggcttg agagccgccg cgagatcgcc
     7561 ggcgagatcg cagccaggat cgaggccgcc gtccgtgccg gacatctgcc cgcgcaggac
     7621 accgctgtgg ctgcgaccgc gctgcttggc gcgctgcatg aatcactggt cgggccgttg
     7681 gcgccggacc atagcgagga tcccgcaaga ttgcgcgacg cggtgcaaac cgtgacgctg
     7741 ctggcgctgc gcgccgtcgg tgtgatggac gcccgcgccc gcggcctggt ggtgcaggcg
     7801 gtactgccgg cgaaaacgct ggtgggagcg taaggtcacg tcatcgttag aagccgatgc
     7861 cgccggcggc tggacatatt ccgccgtatt ggtcgcctaa gagccgctac ttgttcaact
     7921 caccgatgga gcagcgcatg accacgacat tcggatcggc caaatggcag ggtggtatca
     7981 aggacggcaa gggcgcgatc tcgaccaaga gcggcgcgtt gaaggattac ccttacggtt
     8041 tcgccagccg cttcgagggc aagccgggct cgaacccgga agaactgatc ggagcggcgc
     8101 atgccgcctg tttcaccatg gcgctgtcgc tgatattggg cgaagccaag cttaccgccg
     8161 agcagatgga gaccaaggcc gacgtcacgc ttgagaaagt gggcgatggt tttgccatta
     8221 cgtcggtgca tctcgccttg acggcaaaga taccgggcgc ggacaaggcg aaattcgaag
     8281 agctggcggg catggcaaaa gccggatgcc cggtgtcgaa gctgttgaat accaagatca
     8341 cgctggatgc gacgctgctc tgaagcttcg gcaacggaag cgcgatttat ccctccccct
     8401 tgtggcaggg ctatcgcata tgactaaatt gagccgcgcg tgatgcggtc gtggcctcga
     8461 acaaactctc agcgggtcgt ccctgcgaac gcagggaccc ttcacccctg gcctcaaaag
     8521 taaaaaaggc ctttgctcca gtgcaagaac cgagagtccg cggcgtatgg gtccctgcgt
     8581 tcgcagggac gactcgttga gacgccagcc gggaaaattc catatgcgat agccctcccc
     8641 acaaggggga gggaagagct cacatattcg cgtcgtcgtt cgggccgaca gaggcgatgc
     8701 gcagcatgtt ggtggtaccg ggagcgcgaa gcggcacgcc cgcaacgatg atgacgcgct
     8761 ggccggccct ggcaaaacca tcgcgaaatg cgatcgagcc ggcgcggttg accatgtcgt
     8821 ccaggtcttt cgcgtcttcc gcgacgacac aatgcacgcc ccagaccgcg gagagtttgc
     8881 gcccggttgc gaggttcggt gtgatcgcca ccaccggcgg cttcggccgc tcgcgcgcga
     8941 cacgcagcgc ggtcgatcct gaactggtcc agcaaatgat cgcggacaga tcgagcgttt
     9001 cggcgatttg ccgcgccgcg tcggcaatgg cgtcgccgac ggtcggttcc ggttccgcgc
     9061 gctgcgcggt gagcacgccg cgataggtcg gatcgcgctc cacttcctcg ccgatgcgat
     9121 tcatggtcga gaccgcttcg accggaaatt tgccggcggc cgattccgcc gacagcatga
     9181 tcgcgtcggc gccttcatag acggcggttg ccacgtccga gacctctgcg cgcgtcggga
     9241 ccgggctctg gatcatcgat tccagcattt gcgttgcgac caccaccggc ttgccggcgc
     9301 gccgcgccag ccgcgtcatc tgcttctgca ggctcggcac ccgctccagc ggcagctcga
     9361 caccgagatc gccacgcgcc accatcaggg cgtcggatac ttcgatgatt tccggcagcc
     9421 ggtcgatcgc ctgcgg
//
LOCUS       DASWGM010000396         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_305569,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000396 DASWGM010000000
VERSION     DASWGM010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305569"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /gene="sucD"
                     /locus_tag="VGA15_11590"
     CDS             <1..735
                     /gene="sucD"
                     /locus_tag="VGA15_11590"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX9588370.1"
                     /translation="VPVFNTVAEAVRETAANASVIFVPPPYAADAILEAGDAGLPLVV
                     CITEGIPTLDMVRVVAALSGSATRLIGPNCPGIISPGKCKIGIMPGRIHKQGQVGLVS
                     RSGTLTYEAVDQLTRLGIGQSTCIGIGGDPIIGTSFLEAIRLFHDDPETHAIVMIGEI
                     GGNAEEKAAEFIRVSVRKPVVGFIAGQTAPPGRRMGHAGAIISGGQGTAKEKYAAMQA
                     AGIHCVQSPAEIGHTVAAALGKAAAK"
     gene            864..1277
                     /gene="ndk"
                     /locus_tag="VGA15_11595"
     CDS             864..1277
                     /gene="ndk"
                     /locus_tag="VGA15_11595"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9588371.1"
                     /translation="MATERTLAIIKPDAVGRGLEGEILSRIHKAGFRILAMKSLRMTR
                     AEAEGFYAVHRSRPFFAPLTEFMSSGKVVVMVLEAENAIARWRETMGATDPAKAAPGT
                     IRREYGTSIQNNCTHGSDAPETAAFEISYFFPRQI"
     gene            1373..1606
                     /locus_tag="VGA15_11600"
     CDS             1373..1606
                     /locus_tag="VGA15_11600"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX9588372.1"
                     /translation="MELLRELGRILLIFGAVLVAAGAVLLLGARLPFRLGRLPGDIVY
                     QGRHTTFYFPIVTCLLLSVGLTVLFWLLSLFRR"
     gene            1611..>1985
                     /locus_tag="VGA15_11605"
     CDS             1611..>1985
                     /locus_tag="VGA15_11605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX9588373.1"
                     /translation="MTQFQFNLAPERKIWSVSELTGRIRDLLATEFADIWVEGEISNC
                     HAAQSGHLYFTLKDAKAQVRCICFRNQVRLLKFRPEDGLHVTVRGSVGVYEPRGEYQV
                     YVEYIEPVGMGALQLAFEQLKKR"
BASE COUNT          357 a          608 c          646 g          374 t
ORIGIN      
        1 gtgcctgtgt tcaacaccgt cgccgaggcc gtgcgcgaaa ccgccgcgaa cgcttccgta
       61 atcttcgtac cgcctccata tgccgcggac gcgattctcg aagctggcga cgctggcttg
      121 ccgctcgtcg tctgcatcac cgaaggcatc ccgacgctcg atatggtgcg cgttgttgcc
      181 gcactcagcg gcagcgcgac gcgactgatt gggccgaatt gtcccgggat catctcgccc
      241 ggcaaatgca aaatcggcat catgccgggg cgaatccaca agcagggcca ggtcgggctg
      301 gtgagccgca gcggcacgct cacctatgag gcggtggacc agttgacgcg gctcggcatc
      361 gggcaatcca cctgcatcgg catcggcggc gaccccatca tcggcacgtc gtttctcgaa
      421 gccatccgcc tgttccatga cgacccggaa acgcacgcca tcgtgatgat tggcgagatc
      481 ggcggtaacg ccgaggagaa agcggccgag ttcatccggg tgagcgtgcg caagcccgtg
      541 gtcggcttca tcgctggaca gacggcgccg ccggggcgcc gcatggggca tgccggcgcc
      601 atcatcagcg gcggccaggg caccgcgaag gaaaaatacg cggcgatgca ggccgccggg
      661 atccattgcg tccagagccc ggcggaaatc ggacacacgg tcgcagccgc gctgggcaaa
      721 gctgccgcca agtagcgtcg gtgcctcgct gcgatttcat aggtggagcg gcgccctttg
      781 ggccgccatc tgtcatccct tggcagcaca cgacgagcgt cgcgtgataa accgcgttta
      841 ggcatcgcca ggaggggaaa cgattggcga ctgagcgaac gttggcaatt atcaagccgg
      901 atgcggtcgg gcgaggtttg gagggagaaa tcctctcccg gattcacaag gcgggcttcc
      961 gaatcttggc tatgaaatcg ctgcggatga cccgggctga ggccgaaggg ttttacgccg
     1021 tccatcgaag caggccgttt ttcgcgccgc tgacggagtt catgagctcc ggcaaagtgg
     1081 tggtgatggt gctcgaagcg gaaaacgcca tcgcccgctg gcgcgagacc atgggcgcca
     1141 ctgaccccgc caaggccgct cccggcacga tccgccgcga gtacggcacc agcatccaga
     1201 acaactgcac gcacggctct gacgctcccg agaccgccgc ctttgaaatc agctattttt
     1261 ttccgaggca gatttgatcg gctgaccacg ctcggcccgt ctctgggcga agcatctcaa
     1321 ctggtggtgc ctgggacccg ttcggcagta gattcgggta gaatcggcgc caatggagct
     1381 gctgcgcgag ctcggccgga ttttgttgat ctttggcgcg gtgcttgtgg ccgccggcgc
     1441 ggtgctgctg ctgggggcgc ggttgccctt ccggctgggg cggctgcctg gcgacatcgt
     1501 ctatcagggt cggcacacga cgttttattt cccgattgtc acatgcctgc ttctgagcgt
     1561 gggactgacg gtacttttct ggcttctttc attattcagg cgatgaccgg atgacgcagt
     1621 tccaattcaa tctggcgccg gagcgcaaga tctggtcggt cagcgagctg accgggcgga
     1681 tccgcgatct gctggccacc gagttcgccg acatttgggt ggaaggcgaa atctcgaact
     1741 gccatgcagc gcagtcaggg catctctatt tcacgctcaa ggacgcgaag gcgcaggtgc
     1801 gttgcatctg ctttcggaac caggtccgct tgctgaagtt ccgtcccgaa gacggcctgc
     1861 acgtcaccgt gcgcggctcg gtgggggtct acgagcctcg cggcgaatat caggtctacg
     1921 tcgaatacat cgaaccggtc ggcatgggcg ctctgcaact cgccttcgag cagctcaaga
     1981 agcgc
//
LOCUS       DASWGM010000397         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_43982,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000397 DASWGM010000000
VERSION     DASWGM010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43982"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VGA15_11610"
     CDS             <1..342
                     /locus_tag="VGA15_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine synthase"
                     /protein_id="HEX9588374.1"
                     /translation="MKAADNHFRGFPALWNAAAFYLFLLHLSPALSSLAVAALIVLTF
                     VPVHVLHPVRVVRWRGLTLSLIGIWAVLAIFTLASDFDVSASVTIGLCAIAAYIVGSD
                     AAIRLARSFGA"
     gene            339..1091
                     /locus_tag="VGA15_11615"
     CDS             339..1091
                     /locus_tag="VGA15_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9588375.1"
                     /translation="MIELLTSPEAWAALLTLTALEIVLGIDNVIFLSVIVSRIPEARA
                     KRARQIGLALALVFRILLLSLLVWLIGLTQDVISVKGIGFSWRDIILIGGGLFLIGKA
                     TYEIHAEVEARDEENDDAPRASAFFWIIIQIIVIDMVFSLDSIITAIGMAQDLAIMVA
                     AVVIACLIMYLSSGPVARFVGEHPTTKMLALAFLVLIGVALVADGFKFHIPRGYIYFA
                     IAFSAAVEAFNVLAKRNRKKAARPGARRSHGG"
     gene            1155..>1699
                     /locus_tag="VGA15_11620"
     CDS             1155..>1699
                     /locus_tag="VGA15_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9588376.1"
                     /translation="MTKAVRVHKVGGPEALVYEAVDVPAPGPGEVRIRQYAVGLNFID
                     VYYRTGLYKAPGLPFIAGNEAAGEVLAVGPGVTNFHPGDRVAYYFNLGGYASERNIPS
                     EKLVKLPDHITYEQGAVLMLKGLTVWYLLHKTFKVEPGHRVLIHAAAGGIGMLACQWA
                     RALGAHVIGTAGTRAKADLALA"
BASE COUNT          289 a          511 c          532 g          367 t
ORIGIN      
        1 atgaaggccg cggacaatca tttccgcgga ttcccggcgc tgtggaatgc cgcggcattt
       61 tatctgttcc tgctgcattt gagccccgcg ctttcgagcc ttgcagtcgc tgcgctgatc
      121 gtgctgacat ttgttccggt ccatgtgctt cacccggtgc gggtcgtgcg ctggcgcggg
      181 cttaccctgt cgctgatcgg catctgggcg gtgcttgcga tattcacgct ggcaagcgat
      241 ttcgacgtga gcgcgtccgt cacgatcgga ctctgcgcga tcgccgccta tatcgtcgga
      301 agcgatgcgg cgatccggct cgcaagatcg ttcggcgcat gatcgagctg ttgaccagcc
      361 cggaagcctg ggccgcattg ctgaccctga ccgcgttgga aatcgttctc ggcatcgaca
      421 atgttatttt tctgtcggtg atcgtttcgc ggattccgga agcgcgggcg aagcgcgcgc
      481 ggcaaatcgg gctggcactg gcgctggtgt tccgtatcct gctgctgagc ctcctggtgt
      541 ggttgatcgg cctgacgcaa gacgtcattt ccgtgaaggg aattggcttt tcgtggcgcg
      601 atatcatcct gatcggtggc gggctgttcc tgatcgggaa ggcgacctac gaaattcacg
      661 cggaagtgga agcgcgcgac gaagaaaatg atgatgctcc acgcgccagc gcgttcttct
      721 ggatcatcat ccagatcatc gtcatcgata tggtgttctc gctggactcc atcatcaccg
      781 cgatcggcat ggcgcaggac ctcgccatca tggtcgcggc ggtcgtcatc gcctgtctca
      841 tcatgtatct gtcgtccggc ccggtcgcga gattcgtggg cgagcatccg accaccaaga
      901 tgctggcgct ggccttcctg gtgctgatcg gggttgcttt ggtggccgac ggattcaagt
      961 ttcatatccc gcgcggctac atctattttg ccatcgcatt ttcggccgcc gtcgaagcct
     1021 ttaacgtcct ggccaaacgc aaccgcaaaa aggcggccag gccgggggcg agacgctccc
     1081 acggcggttg agttgacaac gcagtcccga tagctttcgt ttgatttcaa gtgaatgcca
     1141 gcggagaacc tgaaatgacc aaggccgtgc gcgtgcacaa ggtagggggc ccggaagccc
     1201 tggtttatga agccgtggac gtgcccgcgc cgggacccgg cgaggtgcgc atccgccagt
     1261 atgccgtcgg cctgaacttc atcgacgttt actatcgcac cggcctctac aaggcgccgg
     1321 ggctgccgtt catcgccggc aatgaagccg ccggcgaagt tttggcggtc gggcccggcg
     1381 tgaccaattt tcaccccggc gaccgcgtcg cctattattt caatctcgga ggctatgcct
     1441 ctgagcggaa tatcccctcg gagaaactgg tcaagctgcc cgaccacatc acctacgagc
     1501 agggcgccgt gctgatgctg aagggactga cggtctggta cctcctgcac aagacattca
     1561 aagtcgagcc gggacatcgg gttctgatcc acgcggcggc aggcggcatt ggaatgctcg
     1621 cgtgccagtg ggccagggcg cttggcgccc atgtcatcgg caccgccggc acccgggcga
     1681 aggccgacct cgcgctcgc
//
LOCUS       DASWGM010000398         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2260640,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000398 DASWGM010000000
VERSION     DASWGM010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260640"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VGA15_11625"
     CDS             <1..541
                     /locus_tag="VGA15_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HEX9588377.1"
                     /translation="QALRKLTASINKSNTMVIFINQIRMKIGVMYGSPETTTGGNALK
                     FYASVRLDIRRIGAIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIMYGEGVSKMGEI
                     LDLGVKAGIVEKSGAWFSYDSQRLGQGRENSKAFLKANPDMTAKIEASIRQNSGLIAE
                     QILAGSPERDAEGEEPAEE"
     gene            complement(967..3840)
                     /gene="gcvP"
                     /locus_tag="VGA15_11630"
     CDS             complement(967..3840)
                     /gene="gcvP"
                     /locus_tag="VGA15_11630"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX9588378.1"
                     /translation="MTMHHKPANGVATSFARRHIGPSPRDISAMLETVGAKSLSALMA
                     ETLPLSIRQTAPLDLGAALSETEALAHMSELAARNQVFTSLIGQGYSGTILPAVIQRN
                     ILENPAWYTAYTPYQPEISQGRLEALFNFQTMICDLTGLDVANASLLDEATAAAEAMA
                     LAERAAPARTKSFFVDREVHPQTLAVLRTRAEPLGWDLVIGDPSLDLDKAEVFGGLLQ
                     YPATSGAIRDLRPAIAALRAKGALAVVAADLLALTLLASPGELGADIAIGSAQRFGVP
                     MGYGGPHAAYMAVRDALKRSLPGRIVGQSIDSRGQPAYRLALQTREQHIRREKATSNI
                     CTAQVLLAVIASMYAVYHGPEGLTQIARNVHRRACVLAAGLRKLGFALQAEAFFDTVT
                     VNMGPRRNEIAARARAGKINLRIGERRLSIALDETTTAATVEAVWRAFGGKLSYADIE
                     ASAPEALPVELRRSTSFLTHPVFHAHRSETELLRYMRKLSDRDLALDRAMIPLGSCTM
                     KLNATSEMIPLTWPQFGSLHPFAPREQAAGYHALFARLEKWLCDITGYDAISLQPNSG
                     AQGEYAGLLAIRGYHAARGEPHRKVCLIPSSAHGTNPASASMAGMEVVVVACDALGDV
                     DVNDLRAKAEKHSNDLAAVMITYPSTHGVFEEHISEICDIVHGHGGQVYLDGANMNAQ
                     VGLSRPGDYGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFLPGHPTIDGAEPR
                     AVGPVSAAPFGSASILTISYIYILMMGGEGLTRASEIAILNANYIATKLDQHFPVLYR
                     NAKGRVAHECIVDPRPLKVSSGVTVDDIAKRLIDYGFHAPTMSFPVPGTLMIEPTESE
                     SKAELDRFCDAMIAIRREIAEIENGRWKVEASPLRHAPHTVHDIADDEWRRAYSRAEG
                     CFPAGTSRTDKYWSPVGRVDNVYGDRNLICSCPPVTEYAQAAE"
BASE COUNT          729 a         1159 c         1259 g          706 t
ORIGIN      
        1 ccaggcgctg cgcaagctca ccgcctcgat caacaaatcc aacaccatgg tgatcttcat
       61 caaccagatc cggatgaaga tcggcgtgat gtacggctcg ccggaaacca ccaccggcgg
      121 caacgcgctg aaattctacg cctcggttcg tctcgacatc cgacgcatcg gcgcgatcaa
      181 ggagcgcgac gaggtggtcg gcaaccagac ccgcgtcaag gtagtgaaga acaagctggc
      241 gccaccgttc aagcaggtcg aattcgacat catgtatggc gagggcgtct ccaagatggg
      301 ggaaattctc gatctcggcg tcaaggccgg catcgtcgag aaatccggcg cctggttctc
      361 ctatgacagc caaaggttag ggcagggccg cgagaactcg aaagccttcc tcaaggccaa
      421 tcccgacatg accgcgaaga tcgaggcgtc gatccgccag aactccggcc tgatcgccga
      481 acagattctg gccggctcgc ctgagcgcga cgccgaaggc gaggagccgg cggaggagtg
      541 agttttatcg ggtttgggtc ggatggcccg ggtttcgaca caggcgggcg ctgaggacgt
      601 tgagttgccg acgtcgccag cgcccgtttt tgtttttgga taaggaagtc cgttaggtgg
      661 accgaattgc ggtgcagggc gcgtgctgac ggtgatgtac tatgagaaga attaatgcag
      721 tcgcgttgtt ggcgacgata tttttcctga attcagtcaa tgtcgggttc acccaggaca
      781 aatgtatcaa gtgcacgcaa aattacgttt acatatgcat gcaaaactat ggtgagtgca
      841 tgaatgcctg cagcagcatc ggaacgaccg ataagaatgc gtgccgacgc cgatgcatcg
      901 cgagcgataa tcggtggaat accagagccg ctttgaagtg cgggacttgt accccaaagc
      961 acttctctat tccgccgcct gcgcgtattc agtaaccggc ggacacgagc agatcagatt
     1021 gcggtcgcca tagacgttgt ccacgcgccc caccggcgac cagtatttat cggtgcgtga
     1081 ggtgcctgcc gggaaacagc cttcggcgcg gctataggcc cgccgccact catcgtcggc
     1141 gatgtcgtgc acggtgtggg gggcgtggcg cagcggcgag gcctcgacct tccagcgccc
     1201 gttttcgatc tcggcaattt ctcgccggat cgcaatcatg gcgtcgcaaa agcggtccag
     1261 ctcggccttg gattcggatt cggtcggttc gatcatcagc gtgcccggca ccggaaagct
     1321 catggtcggc gcatgaaaac cgtaatcgat cagccgcttg gcgatatcgt ccacggtgac
     1381 gccgcttgaa actttaagcg ggcgcggatc gacgatgcat tcatgcgcga cgcggccctt
     1441 ggcgttgcga tacagcaccg gaaaatgctg gtcgagtttg gtcgcgatgt agttggcatt
     1501 gaggatcgcg atttcggaag cgcgggtaag gccttcgccc cccatcatca gaatgtagat
     1561 gtaggagatg gtcaggatcg acgccgaccc aaagggcgcc gccgacaccg gcccgaccgc
     1621 ccgtggctcg gcgccgtcga tggtcggatg tcccggcagg aatggcgcca gatgggcctt
     1681 gacgccgatc ggccccatgc cggggccgcc gccgccatgc ggaatgcaga acgtcttgtg
     1741 gaggttgaga tggctgacat cggcgccgta atcgcccggc cgcgacagcc caacctgcgc
     1801 attcatgttg gcgccgtcga gatagacctg tccgccatgg ccgtgcacga tgtcgcagat
     1861 ctcgctgatg tgctcctcga acacgccatg ggtggaggga taggtgatca ttacggcggc
     1921 aagatcgttc gaatgttttt ctgccttggc gcgcagatca ttcacatcga cgtcgccaag
     1981 ggcgtcgcaa gcgaccacaa cgacttccat ccccgccatg ctggcggagg ccggattggt
     2041 gccgtgggcg gaggagggga tcaggcagac cttgcgatgc ggctcgccgc gcgcggcgtg
     2101 atagccacgg atcgccaaca ggccggcata ttcgccctgc gcgccggaat tcggctgcag
     2161 cgagatcgcg tcatagccgg tgatgtcgca cagccacttt tccagccgcg cgaacagcgc
     2221 gtgatagcct gccgcctgtt cgcgcggtgc gaacggatgc agcgagccga attgaggcca
     2281 ggtcagcggg atcatctccg aggtggcgtt gagcttcatg gtgcaggagc ccagcggaat
     2341 catggcgcgg tcaagcgcga gatcgcgatc cgagagtttt cgcatgtaac gcagcagctc
     2401 ggtctcggag cggtgggcgt gaaacaccgg atgggtgagg aacgaagtcg agcgtctcag
     2461 ctcaacgggc agcgcttcgg gcgcgctcgc ctcgatatcc gcgtagctca gcttgccgcc
     2521 gaatgcgcgc cacaccgcct cgacggtcgc agccgtcgtg gtctcgtcca gcgcgatgct
     2581 gagcctgcgt tcgccaatcc gcaggttgat ttttccggcc ctggcgcggg cggcgatctc
     2641 gttccgccgc ggacccatgt tgaccgtcac cgtgtcgaag aacgcctcgg cttgcagcgc
     2701 aaagccgagc ttgcgcaatc ccgccgccag cacgcaggca cggcgatgta cgttgcgcgc
     2761 gatctgggtc agtccctccg ggccgtgata gaccgcatac atcgaggcga tcaccgcaag
     2821 aagcacctgc gcggtacaga tgttggaagt cgccttttcg cggcggatat gctgttcgcg
     2881 ggtctgcagc gccagccgat aggcgggctg ccctcgcgaa tcgatcgact gcccgacgat
     2941 gcggccgggc agcgagcgtt tcagggcgtc gcgcaccgcc atataggcgg catgcggtcc
     3001 gccatatccc atcggcactc caaaacgctg tgccgaaccg atcgcgatat cggcgccgag
     3061 ctcgccgggc gaggccaaca gcgtcagcgc cagcagatcg gcggcgacga cggccagcgc
     3121 gccctttgcg cgcagtgctg cgatcgcggg gcgcaggtcg cgtatcgcgc ccgaggtggc
     3181 cggatattgc agcaacccgc caaatacctc ggccttgtcg agatccaggg aagggtcgcc
     3241 aatgacaaga tcccacccca acggttcggc gcgggtgcgc agcaccgcca gggtctgcgg
     3301 atgcacttcg cggtcgacaa agaacgattt tgtccttgcc ggcgcggcgc gctcggcgag
     3361 cgccatcgcc tctgccgccg cggtcgcctc gtcgagcagc gaggcgttgg cgacatcgag
     3421 gccggtgagg tcgcagatca tggtctggaa gttgaataaa gcttccagcc ggccctggct
     3481 aatctccggc tgatacggcg tataggccgt ataccacgcc ggattctcca aaatgttgcg
     3541 ctggatcacc gccggcaaga tcgtgccgga atagccttgg ccgatcagcg aggtgaagac
     3601 ctggtttcgc gccgcaagct cgctcatatg cgcgagcgct tcggtttcgc ttaaggcggc
     3661 gccgagatcg agcggcgccg tctgccggat cgacaacggc agggtttcgg ccatcagcgc
     3721 gctgaggctc tttgcgccca ccgtttcgag catcgctgaa atgtcacgcg gcgaggggcc
     3781 gatgtggcgg cgcgcgaagc tggtggcgac gccattggcg ggtttgtggt gcatggtcat
     3841 ggggtgttcc tcg
//
LOCUS       DASWGM010000399         5590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_444785,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000399 DASWGM010000000
VERSION     DASWGM010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444785"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VGA15_11635"
     CDS             complement(<1..1341)
                     /locus_tag="VGA15_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9588379.1"
                     /translation="MLIPIADVPRWYAERKHKDAVAISHGADTLTWEQLERGANRRAR
                     AFAARGVKPGDFVAIGLPNSNAFFETTFAVWKCGATPTSLSWRLPRGEAAAVLEILKP
                     ALVVGGEADWNAPNSVPADFVPEGFSDETLNNPVARYWKAMTSGGSTGRPKVILDHGP
                     AVVDTAADQMLGIPRGVSLLNPGPLYHNAPFILSHMGLFAGGRMTGMVKFDAEEALRL
                     IERNRVQWVNFVPTMMHRIWALPEQVRNAYDLSSLQIVFHMAAPMPPWLKEKWIGWLG
                     PERIWELYGGTERQGATVISGVEWLAHRGSVGRIGESSSLRIIGEDGNDVAPGETGEI
                     YFLPNDGAGSTYHYLGAEPKRRPDGWESLGDIGRLDADGYLYLGDRLADMILRGGANI
                     YPAEVEAAVMAHPHVRSCVAVGLPDPEFGQRVHAILELAGDAEAQSVIDGMGGFL"
     gene            1890..2936
                     /locus_tag="VGA15_11640"
                     /pseudo
     CDS             1890..2936
                     /locus_tag="VGA15_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            3721..5139
                     /locus_tag="VGA15_11645"
     CDS             3721..5139
                     /locus_tag="VGA15_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX9588380.1"
                     /translation="MDNRNDIWRGVDAIKPRFIALSDKVWAMPEVCYTEARSAAEHLA
                     ELRHQGFRITENVAGIPTAAMGEWGEGGPVIAFLGEYDALPGLSQEAGVAEPRPLEVG
                     GHGHGCGHNLLGSAALLAATAAKDWLAAHKVPGRVRYYGCPAEEGGAAKTFMVRSGAF
                     DDADIAITWHPSSFWEVVVTPSLANTRADFIFTGRASHAAASPHLGRSALDAVELMNV
                     GVNYMREHMPSDARVHYALLDTGGIAPNVVQAHARVRYSIRARDLSGMNELVERVHKI
                     AQGAALMTETKMEMRIISAVSNVLPNTPLEQTLHRIMEDLGPPRFDQADQEFAAKIRS
                     TLTEKDIASVYHAIGMDPTDRPLADFIVPLDARRNPLIGSTDVGDVSWIVPTVQVHAP
                     TVAIGTPFHTWQVVAQGKSPAAHKAMVQAAKAMAGLGVKALNEPDLIAAAKADLKKRT
                     ARTPYVSPLPDKVAPPLDMSLT"
     gene            complement(5216..>5590)
                     /locus_tag="VGA15_11650"
     CDS             complement(5216..>5590)
                     /locus_tag="VGA15_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX9588381.1"
                     /translation="QLPSVVSLKSFVKPTTHPAFTRFNVFLRDRFVCQYCSANDDLTF
                     DHIVPRSKGGQTTWENVVAACSPCNLRKGNLTPQQARMFPRQLPFAPTVHQLHRNGRL
                     FPPNYLHDSWLDYLYWDTELDP"
BASE COUNT         1045 a         1776 c         1759 g         1010 t
ORIGIN      
        1 caggaagccg cccatgccgt cgatcaccga ttgcgcctcg gcgtcgcctg caagttccag
       61 gatggcgtgg acgcgttgtc cgaattcagg atcgggcagg cctacggcga cgcaggagcg
      121 cacgtgcgga tgcgccatca cggcggcctc gacctcggcc ggatagatat tcgcgccgcc
      181 gcgcaggatc atgtcggcaa ggcgatcgcc gagatagaga tagccgtcgg cgtccagcct
      241 tccgatgtcg ccaagcgatt cccagccgtc ggggcggcgc ttcggctcgg cgccgagata
      301 atgatacgta ctgccggcgc cgtcgttggg caggaaatag atttcaccgg tctcgcccgg
      361 cgcgacgtca ttgccgtcct cgccgataat ccgcaagctg gaagattcgc cgatcctgcc
      421 gaccgagccc ctgtgcgcca gccattcgac gccggatatc acggtggcgc cctgccgctc
      481 ggtgccgcca tagagttccc agattcgctc agggccgagc caaccgatcc atttttcctt
      541 cagccatggc ggcatgggag ccgccatgtg aaacacgatt tgcaggctcg acaggtcata
      601 ggcgttgcgc acctgctccg gcagcgccca gatccgatgc atcatggtcg gcacaaagtt
      661 gacccattgc acccgattgc gctcgatcag gcgcagcgct tcctcggcat cgaatttcac
      721 cattcccgtc atacgcccgc ccgcgaacaa tcccatgtgc gacaggatga acggcgcatt
      781 gtgatagagc gggccgggat tgagcagcga cacgccgcgc gggatgccga gcatctggtc
      841 cgccgccgtg tccacgaccg cgggcccgtg atcgaggatc accttgggcc gtccggtcga
      901 gccgcccgag gtcatcgcct tccagtagcg cgccacgggg ttgttcagcg tttcgtcgga
      961 aaaaccttcc ggcacgaaat cggcgggcac cgaattgggc gcattccagt cggcttcgcc
     1021 gccgaccacc aaggcgggtt tgagaatctc cagcaccgcg gcggcttcgc cgcgcggcag
     1081 ccgccacgat agcgaggtcg gcgtcgcgcc acacttccac accgcaaagg tggtttcgaa
     1141 aaaagcattg ctgttgggca ggccgatggc gacgaaatca cccggtttca cgcctcttgc
     1201 ggcaaaggcg cgggcgcggc ggttggcgcc gcgctcgagc tgctcccagg tcagcgtgtc
     1261 cgcgccatgg ctgatcgcaa ccgcgtcctt atgcttgcgt tcggcatacc agcgcggcac
     1321 gtcggctatt gggatcaaca tgagggcgtt tccgttacag ggcgtcttga tgccgctctt
     1381 gtgtttcgaa caggtggcgc gttgccgcga caggtgcgtc attcaccaac agggactgcc
     1441 gtgagtcaat gggacacaaa ggatcgtcac gttaccccaa atggggcaga acgatcaggg
     1501 atgtggcgct ttcaaaatat cggggaaatc gatttaatcc cggtttgcaa ccatccgggg
     1561 acgtgccgat gtacccgtgg attgaatgag ccgcgccgat gtcgtcgaca aagcagaatt
     1621 tcaggcgtca cgttacggag aaaaatatgg cgcgccacct gtgctctctg gcgttcattc
     1681 tcgttgtagc tggctccccg ccatcggcga acgcccagga cgcgacgacg gcggggagcc
     1741 gtggcggcga ggcggtgcct ggagccgtat tttcggatac cggccccgat gcagcggcct
     1801 atggcgcagc ggcaggcttt ccggttggca cgcgagcgac ggccagtcag cttgaaaacc
     1861 tcgtgggtac cttcagccat ttcgatgaga tgttttcatc acgcccgata cggcgcgcga
     1921 cgacgccctg gctgttcaag cgggcacctg agccggcgat ctcctacaat ttcggatcgg
     1981 atcggctctc gatcgagaac tatcttggcc gtaacccgac gaccgggctt ttgatcgcaa
     2041 aggacgacac gattctgtac gagcactatc aatacgcacg caccgatcgt gaccgcttcc
     2101 tctcgcagtc catggcaaag acgataacgg cgatgctgat tggcgtcgcg gtctccgaga
     2161 acaagatcag gtcgatcgac gacctcgtct cagtctacgt ccccggcctc gcgaatacag
     2221 aatacggaaa gacgcccatt cgggacctgc tgcacatgtc gtccggcgtc gccttttcgg
     2281 aaaactatga tgggaaggat gatgtcgcgc agctcgcgcg cgatctattc ggtgagcctg
     2341 gcaaggatcc cattgccagc gttgcccaat tcaacacccg catcgctcca ccgggaacca
     2401 aatggcacaa cgccagcgtc gagacggaaa tcctgggcct agtgtagcgg gccgccacgg
     2461 gcgtgcccgt tgcggattat cttggcgaca agatctgggg cgcgatcggc accgaggccg
     2521 acgcttcgtg ggccatcgac ggcgccgggc aggaaactac cttctgctgc ttcaacgcaa
     2581 ccttgcggga ctatgcgcgg ctgggtcggc tgctcgccca tgacggcgcg tgggagggac
     2641 gccaactgat tccacggcag tggctcctgg acgcgacgac ggtgaatccg agcaacgcct
     2701 atctcgctcc gggagccgcc accccatact tcggctatgg ataccaggta tggatcctgc
     2761 ccggcgagca gcggagtttt gccctgatcg gaatccgggg ccaaatgatc ttcgtagatc
     2821 ctgcctccaa gctcgtgatg gtgcacgccg ccgtgcgcaa gaaaccgtcg gacccggcct
     2881 cttatgcgga tcccattgcc ctatggctcg gcgtggtcgc gcagctggga aaatgatcgt
     2941 cggccatcgc ggcttcgcac gcccaagcag atgctcgatc gcgaccggcg ccggcgccga
     3001 agtccagcag gaccctcggc ccatcaccac cacaactgat tgggttgccc gtcgggcaaa
     3061 tcacaccatc ggccggtcca gtcccttatc caaaaatatt cctgttttcg tctccccaaa
     3121 tcagttctag aagcctcgtc atcccgtccc gcaacgaggg gcgtatccgg atcgtcaagt
     3181 acgttgggat gggatgcggt ggacgcggca gcgtcgggcg cgcaagggat cgcagggctg
     3241 actccggtct gtgagcgaac aaccggcgcg acagacgaac gacgttgcgg cgtacggcaa
     3301 agccgtgtgg tcctggcacc cgttgctggt gtcaagttgg cggaggttca cgagcccaac
     3361 cgggctttga tgggccatta atccaccaac gacggtgaca agacgaattc gtcgccgggg
     3421 agagcgcgga ggaaaccgtt aaaaccattg cgtgcgggaa tgccgggtgt ttccggcgtg
     3481 ctcgtggtga ctaccctgtg cttactacac tgcacagggg ctgcgggtgc gcgggcaccc
     3541 ggcattcccc acgccctcat tgggcgagag atagacgcaa acctcgggcg catcgcgccg
     3601 cgagatcgtc gacgcatatc cttcgcgccg gtcgcatggc tggtgccccc ggccggcgct
     3661 aggcggggtc tggcgaagct gataacgatt tcactctcac atcggatcgg gggccggagc
     3721 atggacaacc gcaacgatat ttggcgtggc gttgacgcga tcaagccgcg tttcatcgcc
     3781 ttgagcgaca aggtctgggc gatgcccgag gtgtgctaca ccgaggcgcg ctccgccgcc
     3841 gaacatctgg ccgagctgcg ccatcagggt tttcgcatca cggagaatgt cgccggcatt
     3901 cccacagccg caatgggcga gtggggtgag ggtggacccg tcatcgcttt cctcggtgaa
     3961 tacgatgctc tacccggtct aagccaggag gcgggcgtgg ccgagcctcg cccgctggag
     4021 gtgggaggcc atggccacgg ctgcggccac aatctcttgg gctcggccgc gctgcttgcc
     4081 gccacggccg cgaaggattg gcttgccgct cacaaggtgc cggggcgcgt gcgctattat
     4141 ggctgccccg ctgaggaggg gggtgctgcg aaaaccttca tggtgcgctc aggcgccttc
     4201 gatgacgccg acatcgccat cacctggcac ccgtccagct tctgggaggt ggtggtaacg
     4261 ccttcgctgg ccaacacgcg cgccgatttc atcttcaccg ggcgcgcctc gcatgccgcg
     4321 gcctcgcccc atctgggccg cagcgcgctc gacgcggtgg aactgatgaa tgtcggcgtg
     4381 aactatatgc gcgagcacat gccgagcgac gcccgcgtgc actatgctct gctcgacacc
     4441 ggcggcattg cccccaatgt ggtgcaggcc cacgcccgcg tgcgctattc catccgcgcc
     4501 cgcgatctct ccggcatgaa cgaattggtg gagcgtgtcc acaagatcgc gcagggggcg
     4561 gctctgatga ccgagaccaa gatggagatg cggatcattt ccgcggtctc caatgtcttg
     4621 cccaacaccc cgctcgagca aaccctccac cgcatcatgg aggatctcgg tcccccgcgt
     4681 ttcgaccagg cggaccagga attcgccgcg aaaatccggt ccactctgac cgaaaaggac
     4741 atcgcttcgg tctatcacgc gatcggcatg gatcccaccg accgcccgct ggccgatttc
     4801 atcgtgccgc tggacgccag acgcaatcct ctgatcggct cgaccgatgt gggcgatgtg
     4861 agctggatcg tcccgaccgt tcaggtccat gccccgacgg tcgccatcgg caccccgttc
     4921 cacacctggc aggtggtggc gcagggcaag agcccggccg cccacaaggc catggtgcag
     4981 gccgccaagg ccatggcagg gctcggcgtc aaggcgctta acgagccgga cctgatcgca
     5041 gccgccaagg ccgacctcaa gaagcgcacc gcccgcacgc cctatgtcag cccgctgccg
     5101 gacaaggtcg cgccgccgct ggacatgtcc ttgacctgag ctttgacctg atttggcgaa
     5161 caaatttttc gcaagagagg agcaagagag gagcgacaaa atccgcgggc aaaccttacg
     5221 gatcgagttc ggtatcccaa taaagataat ccagccagct gtcgtgcaga tagtttggcg
     5281 ggaacagccg gccgttgcgg tgcagctgat gcaccgtcgg tgcgaacggc agttgccgcg
     5341 gaaacatccg cgcctgctgc ggcgtcaggt ttcccttgcg cagattgcac ggcgaacatg
     5401 ccgcgaccac gttttcccag gtggtctggc cgcccttgct gcgcgggacg atgtgatcga
     5461 aggtgaggtc gtcgttggcc gaacaatact ggcagacgaa gcggtcgcgc aggaacacgt
     5521 tgaaccgggt gaaggcggga tgcgtggtcg gcttgacgaa ggatttcagc gacaccacgc
     5581 tcggcagctg
//
LOCUS       DASWGM010000400         4821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_44167,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000400 DASWGM010000000
VERSION     DASWGM010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44167"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGA15_11655"
     CDS             <1..217
                     /locus_tag="VGA15_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX9588382.1"
                     /translation="IAAAAMTAFKADGITLQQFNEAAGGQVVFHLHMHVMPRHDGIAL
                     LPPASRKEDAKVLEENAARLIAALKGA"
     gene            complement(324..815)
                     /gene="tsaA"
                     /locus_tag="VGA15_11660"
     CDS             complement(324..815)
                     /gene="tsaA"
                     /locus_tag="VGA15_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX9588383.1"
                     /translation="MVRESEIREGELAVEMPPARDAGLVFIGRIRTPWTSRLDAPRQG
                     RHDGPVCRLEIFEPWVPALKGVDFYSNLEVIYWLHLSRRDLVLQSPKNNKSTRGTFSL
                     RSPVRPNPIGTSIVKLVGIEGNTVLVRGLDCLDETPLLDLKPDRCEFTPLAPPQAGDF
                     ETE"
     gene            complement(819..1541)
                     /locus_tag="VGA15_11665"
     CDS             complement(819..1541)
                     /locus_tag="VGA15_11665"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9588384.1"
                     /translation="MDTRCSWRPVRSLCKIRCSTKSCPTTRQKIFGCWRWSPICPLSW
                     RCIRRCRRKTVAEFVAYAKQNPGKLNFGSAGTGGTIHLAGEMFKQMAGIEMTHVPYKG
                     AGPALTDLLSGNIQVMFDSLSTALPPVKSGLLRPLGISSAQRSPDLPDVPTIAESGYP
                     NYTVSVWYGIAAPSKLPDDVAQKISASLDRALNDDVFRASLEKIGFPVFRPRGAAAIQ
                     EFIDADRARWSAVIKAQNISLD"
     gene            complement(1441..1761)
                     /locus_tag="VGA15_11670"
                     /pseudo
     CDS             complement(1441..1761)
                     /locus_tag="VGA15_11670"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
     gene            complement(1891..2235)
                     /locus_tag="VGA15_11675"
     CDS             complement(1891..2235)
                     /locus_tag="VGA15_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX9588385.1"
                     /translation="MSDFIGDWHALLILLIAGVIPNQIWRMLGLWLGGGIDEGSELLI
                     WVRAVATAILAGVIAQILVQPPGALASVPGWLRYSAVVAGFAVFMATRRSIFAGVVCG
                     GIVMLSGKWWLG"
     gene            complement(2232..2969)
                     /locus_tag="VGA15_11680"
     CDS             complement(2232..2969)
                     /locus_tag="VGA15_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX9588386.1"
                     /translation="MPLPPLDSPKWQSSLRAFAWGMRSVASTVLTLVLFATYLGIGAL
                     AHDTHFSLAWALASTAFVWAGPAQIILISTLGSGATVVQAAIAVTVSAIRLFPMVVSV
                     LPMMRTPQTKRRHLVLVAHLTAVTLWVECFRFLPHVPRERRIAFIHGLGGGLVSVCLV
                     ATAIGYGLAANLSQLFAAAILMLTPLAFLFSTARNCRELADGVALALGLVLFPLAAML
                     NSGIDILVSGVSAGTIAYGVHRWRARP"
     gene            complement(3066..3260)
                     /locus_tag="VGA15_11685"
     CDS             complement(3066..3260)
                     /locus_tag="VGA15_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588387.1"
                     /translation="MGKDHFDTLPAARRDAVRSALAAAFGRALTPGAPETKHILGKMF
                     LMSFLSGVAPPGFDDAVQQG"
     gene            complement(3328..4251)
                     /locus_tag="VGA15_11690"
     CDS             complement(3328..4251)
                     /locus_tag="VGA15_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9588388.1"
                     /translation="MIGGGVARPSVEELAIPLDENNAEPQKPPETARDTDTREAMCLM
                     IESAAKAQDLPLEFFARVIWQESRFQSDAVGPVTRSGQRALGIAQFMPGTANERRLLD
                     PFDPVQALPKSAEFLSELRQQFGNLGLAAAAYNAGPRRVQEWLAGSGPMPQETRNYVI
                     AITGASVDDWAAAAKNGNKNGKTLDRAPTSSCRELMALLKRAPNPFVTQLEQHVKLGA
                     DRLWGVQLAAGFNRDKALAMYSRAMKQLSAVIGDRDPSLLGTMLRSRGTRIFYQVRIG
                     TDTRPAADDLCNRIRRAGGACFVLRNMNVRG"
     gene            4602..>4821
                     /locus_tag="VGA15_11695"
     CDS             4602..>4821
                     /locus_tag="VGA15_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9588389.1"
                     /translation="MLDRRQSTRDKVIYGGVAEIDERGTTTDCVVRNISEQGASLEFS
                     SAVKLPKGRISLTIAQKGRSFLAKIIWWR"
BASE COUNT          964 a         1544 c         1494 g          819 t
ORIGIN      
        1 aatcgcagcc gccgcgatga ccgccttcaa ggccgacggc atcacgttgc agcaattcaa
       61 cgaagccgcc ggcggacagg tggtgtttca tctgcacatg catgtcatgc cgcgccacga
      121 cggcatcgcg ctattgccgc ccgcgagccg caaggaagat gcaaaagtgc tggaagagaa
      181 tgcggccagg ctgatcgcgg cgttgaaggg ggcgtagagg agccgtcacc ctgaggtgcg
      241 cgcttttgcg cgcctcgaag ggcggcggcg gtggcacatc cttcgaggct cgccgaagag
      301 gctcgcacct caggataacg gtctcactct gtctcgaaat ccccggcctg tggcggcgcc
      361 agcggggtga actcgcagcg gtcgggtttg agatccaaca gcggcgtctc gtcgaggcaa
      421 tcgaggccgc gcaccagaac cgtattgccc tcgatgccga ccagcttgac gatcgatgtg
      481 ccgatcgggt ttggccgcac cggcgaacgc aacgagaatg tgccgcgggt cgatttgttg
      541 tttttcgggc tttgcagcac gaggtcgcgg cgcgacagat gcagccagta gatcacttcg
      601 agattggaat agaaatcgac gcccttcagc gccggcaccc acggctcgaa aatctccagc
      661 cggcagaccg ggccatcgtg acgcccttgc cgcggggcgt cgagacgcga ggtccagggc
      721 gtgcggatac ggccgatgaa gaccaggccg gcatcgcgcg ccggcggcat ctcgacggcg
      781 agttcgcctt cgcggatttc actttctcga accatgtttc aatccaatga aatgttttgc
      841 gccttgatca cggccgacca gcgtgcccgg tcggcgtcga tgaattcctg gatggcggcg
      901 gcgccgcgcg gacggaacac gggaaaaccg atcttttcaa gcgatgcccg gaacacatca
      961 tcgttaagag cccgatcgag gctcgcgctg attttttgcg cgacatcgtc gggcagcttg
     1021 gacggcgcgg caatgccata ccagacgctg acagtatagt taggatagcc gctctcggcg
     1081 atggtcggca cgtcaggcag atcggggctg cgttgcgctg agctgatacc gagcggccgc
     1141 agcaatccgg attttaccgg cggcaacgcg gtgctcagcg aatcgaacat cacctggatg
     1201 ttgccggaca acagatctgt caatgccgga cccgcgccct tatagggtac atgggtcatc
     1261 tcgatgcccg ccatctgctt gaacatctcg ccggcgaggt gaatggtgcc ccccgtccct
     1321 gccgagccga aattgagctt gccgggattt tgcttggcgt aggcgacgaa ctccgccacc
     1381 gtttttcgcc ggcaccgacg gatgcacctc catgacaacg ggcaaatcgg tgaccagcgc
     1441 cagcatccga aaatcttttg acgggtcgta ggacagcttt ttgtagagca tcggattttg
     1501 cacaatgatc gaaccggccg ccacgaacag cgtgtatcca tccggttcgg cgcgcgctac
     1561 cgcttccgcg ccgatcgcgc cttgcgcgcc cggcttgttc tcgacgatgg tcgtctgctt
     1621 gaggtcccgg ccgagataat cggccaccac ccggccaaga acgtcggtcg gaccaccggc
     1681 ggcatagggg acgaccagct tgatcgggcg aacgggataa tcaacagcca tcgcgtccgg
     1741 cgcagcaaat gccaccgtca tcgccagacc ccaggcgtag atgaatcttg cttgcgccct
     1801 cacatcgtct cccgttgtct tgttatgttg cggcaacact atccgaggcg ggaccgggag
     1861 caaggccgaa ttgccgcgac gtggtaagac tcagccgagc caccacttgc cactcagcat
     1921 cacgatcccg ccgcagacca caccggcaaa aatcgaccgc cgcgtcgcca taaaaaccgc
     1981 gaagcccgcg accaccgcac tgtaacgcag ccatcccggc acgctcgcca gcgcgcctgg
     2041 cggctgcacc aggatctgcg cgatgacgcc ggcgagaatg gcggtcgcca ccgccctcac
     2101 ccaaatcagc agctcggacc cctcgtcgat gccgccgcca agccaaagcc cgagcatgcg
     2161 ccatatctga ttggggatga cacccgcgat cagcagaatc agcaacgcat gccagtcgcc
     2221 gataaaatcg ctcatgggcg cgctcgccac cggtggacac cataggcgat ggtgccggcc
     2281 gagacgccgc tgaccaggat atcgatgccg ctgttcaaca tcgccgcgag cgggaacagc
     2341 accagcccca gcgccagcgc cacaccatcc gcgagttcac gacagttgcg ggcggtggag
     2401 aacagaaatg ccagcggcgt cagcatcagg atcgcggccg cgaatagttg cgagagattg
     2461 gccgcgagcc cgtagccgat cgcggtcgca accaggcaga cggaaacgag accgccgcca
     2521 agcccatgga tgaacgcgat ccgccgttcg cgcggcacgt gcggcaggaa gcgaaagcat
     2581 tcgacccaca aggtgaccgc cgtgagatgg gccaccagca ccaggtgacg ccgctttgtc
     2641 tgtggcgtgc gcatcatcgg caatacggaa accaccatcg gaaacagcct gatggcgctg
     2701 acagtcacgg cgatggccgc ctgcaccacc gtcgcacccg accccagcgt cgagatcagg
     2761 atgatctgcg ccggccccgc ccagacaaag gccgtgcttg ccagcgccca tgccaggctg
     2821 aaatgcgtgt cgtgcgcgag ggcgccaatg ccgagatagg tcgcgaacag caccagcgtg
     2881 agcaccgtcg aggcaaccga gcgcattccc catgcgaacg cgcgcagcga gctttgccat
     2941 ttaggagagt ccagcggggg aagcggcacg tatatccggg caatgtgagt cgtggcgggc
     3001 ataacgctgg tgaaaccagc cgacgtcaag cgagccgcag gcagatcagc catcctgcca
     3061 cggcttcaac cttgctgaac ggcgtcgtcg aatccgggcg gcgcaacgcc ggacagaaat
     3121 gacatcagga acatcttgcc caggatgtgc ttggtctccg gcgcgccggg cgtgagagcg
     3181 cggccaaagg cggcagcgag cgctgagcgc accgcgtcgc ggcgcgcggc gggcagcgta
     3241 tcgaaatggt ctttgcccat gactgtttct tactacatgg cgcggacgtg tctaagccca
     3301 gttcctgccg gaccgccgcg ctaccgatca gcccctgacg ttcatgtttc gcaacacgaa
     3361 acaggcgcca cccgcgcgac ggatgcggtt gcagagatcg tccgcggcag gacgcgtgtc
     3421 ggtgccgatc cgcacctgat agaagatgcg ggtgccgcgg ctgcgcaaca tcgttccgag
     3481 caaacttggg tcgcgatcgc cgatcacggc gctcagctgc ttcatcgcgc gtgaatacat
     3541 cgccagcgcc ttatcacgat tgaaaccggc ggccagctgc acgccccaca gccggtctgc
     3601 cccgagcttc acatgctgtt ccagctgggt gacgaaggga ttgggtgcgc gcttgagcag
     3661 ggccatcaac tcgcggcagc tggaggtggg cgcgcggtcg agcgtcttgc cattcttgtt
     3721 gccattctta gcggcagcgg cccagtcgtc gaccgatgca ccggtaatgg cgatcacgta
     3781 gttgcgggtc tcctgcggca tagggcctga tccggcgagc cattcctgca cccgccgcgg
     3841 tccggcattg taggcggcgg cggcaagccc gagattgccg aactgctgac gcaattcgct
     3901 caaaaactcc gcggatttcg gcagcgcctg taccggatcg aagggatcga gcagccggcg
     3961 ttcattggcc gttcccggca tgaactgcgc gatgcccagc gcgcgctgac cgctgcgggt
     4021 gacgggtccg accgcgtcgg attgaaagcg gctttcctgc cagatcacgc gggcgaaaaa
     4081 ctccagcggc agatcttgcg ccttggccgc cgattcgatc atcaggcaca tcgcctcgcg
     4141 ggtgtcggta tcccgcgcgg tctccggggg cttttgcggc tccgcgttgt tttcatccag
     4201 gggaatcgcg agttcttcga cgcttggcct tgcaaccccg ccgccgatca tcgcaggcgc
     4261 cgcatcttgc gcactcgccg gcccaaagca cgccgcgaat gcgaagcaaa gtgcggcatg
     4321 cagaccaatg gcagttcgca ttgtcgctct gctttccgtg agaccgagcc tgacaccggg
     4381 aaccgacggt ctagccattt aaaaatgatg gcgcgtgcgt gggaacgcaa gacatgacca
     4441 caacgcttga cgaccatctt cccgccgact ggaagagctc gaaaattcac gctgtcccgg
     4501 accgaaatcg gtttaggtta acgccgcatt ttgcagcttg cggtctttcc cggccgacgt
     4561 agcggcgtat ggtcccgttc aataatcggg gaggaaattc gatgttggat cgacgccaga
     4621 gcactcgcga caaggtgatc tatggcggcg ttgccgaaat cgacgagcgt ggcacaacca
     4681 cagactgcgt tgtccgcaac atttccgaac aaggcgccag cctcgaattc agcagcgccg
     4741 tcaagctgcc caaagggcgg atatcgctga ccatcgcgca aaaaggccgt tccttcctcg
     4801 ccaaaatcat ctggtggcgc g
//
LOCUS       DASWGM010000401         6463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2399658,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000401 DASWGM010000000
VERSION     DASWGM010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399658"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /gene="rpsT"
                     /locus_tag="VGA15_11700"
     CDS             complement(<1..243)
                     /gene="rpsT"
                     /locus_tag="VGA15_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX9588390.1"
                     /translation="MANTTSAKKATRKIARRTTINKSRRTQMRGAVRIVEEAIKSGDR
                     DAALKAMTRAEPELMQAAQRNIIHKNNASRKVSRLAH"
     gene            599..913
                     /locus_tag="VGA15_11705"
     CDS             599..913
                     /locus_tag="VGA15_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588391.1"
                     /translation="MIRGFFRLVGLLLLAGGFIFMVYDGARWVADQTLRFTRFGEFWN
                     DIHQSSQQAFRAWVEATAPWLWTSVKIILDQPVFAVMGVLGILLMLLFRPRKPLIGYS
                     RN"
     gene            1026..1682
                     /gene="msrA"
                     /locus_tag="VGA15_11710"
     CDS             1026..1682
                     /gene="msrA"
                     /locus_tag="VGA15_11710"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9588392.1"
                     /translation="MLFMRKTTALPSAAEALPGRAKPIPTATSHFVNGRPLQPPYPAG
                     LEQAVFGLGCFWSAERKFWQLGDGIYTTAVGYAGGHTQNPSYEEVCSGLTGHTEAVRV
                     VFDPNKVSYDQLLKTFWESHNPTQGMRQGYDVGTQYRSAIYTFNDAQRKAADASKSAY
                     QRALAAKGLGAITTEIAPSGEFYFAEDYHQQYLAKNPTGYCGLGGTGVSCPIGAGVSA
                     "
     gene            complement(1753..2424)
                     /locus_tag="VGA15_11715"
     CDS             complement(1753..2424)
                     /locus_tag="VGA15_11715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9588393.1"
                     /translation="MGQDIKLTASDNFQLGGYRADPATAPKAAVVVIQEIFGVNHHIR
                     AVCDRLAGEGYVAIAPAIFDRVEPNFTSGYSPDEIAVARKFVANPDWAAMLRDTQAGI
                     DAVSHVGPVGIIGFCLGGSIAYAAATKLSGLSAAVGYYGGAIVRFADDKPSVPTQLHF
                     GEKDAGIPLADVETISARRPEVEIHVYPGAQHGFHCDERASYDKTSADIAWPRSLGFF
                     ARHLK"
     gene            complement(2499..2801)
                     /locus_tag="VGA15_11720"
     CDS             complement(2499..2801)
                     /locus_tag="VGA15_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588394.1"
                     /translation="MDAAAAQTFVPAGGSNAPPFTATLSNNTPLVFGMDAEDAARALG
                     TPLNYISGRPGDETLLAFRNVGGSGLFFHKDRLYLQFRKGRLTGWKGDWGHNWMWQ"
     gene            complement(2852..3646)
                     /locus_tag="VGA15_11725"
     CDS             complement(2852..3646)
                     /locus_tag="VGA15_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9588395.1"
                     /translation="MPKINRDGVQIYYEVHGSGPPLLLTHGYSSTSQMWQGQIEALSR
                     HHKLVLWDMRGHGRSDYPDEASAYSEALTVADIAALLDEVGAISAIVGGLSLGGYMSL
                     AFYRAHPERVRALLIIDTGPGFKKDDAREAWNKRALDTGDRFEREDLEVLKSSGRERS
                     MVSHRDASGLARSARGMLTQRDARVIESLPDIKVPALVVVGADDTPFLAAADYMAAKI
                     PGAKKAVIPAAGHAVNIDQPQAFIDAVLPFLDSLPRSAVAERMVPS"
     gene            3996..4484
                     /locus_tag="VGA15_11730"
     CDS             3996..4484
                     /locus_tag="VGA15_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9588396.1"
                     /translation="MPTLNINGRNMSVEAANDTPLLWAIREQLQMTGTKFGCGAGLCG
                     ACTVHVNGEAVRSCQTPLSDVAGKKITTIEGLSAKGDHPLQKAWIAEQVPQCGYCQSG
                     QIMQAAALLSKNANPTKDEVVAHMDGNLCRCMTYSRIQKAIMRAASEMRTASTSTERR
                     AT"
     gene            4481..>6463
                     /locus_tag="VGA15_11735"
     CDS             4481..>6463
                     /locus_tag="VGA15_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9588397.1"
                     /translation="MNTHVKITKDIVDREPADLSRRSFLVGSAAAGLALGYSAVPGLV
                     GADQAFAAASNFDPSVWYSIAPDGIVTVTCGKADMGQHIASTMAQIISEELGSNWKDM
                     RVQLASNDPKFNDPVLGAQITGGSWSTMMNFDAMSRAGAAGRMALTEAASAAMGVPAG
                     ELVVRDSVISHPKSKKSMTFADVVKSGKATKTFTPDELKAIQLKTPDQYTKIGVSVPQ
                     LDIPSKTNGTAKYGIDVMLPGMVYGRVVTPPVRFGATVKSVDDSAAKKVPGFIKAVTL
                     DDQTGSTTGWVVAVANTYSNAIKAADALKITYDGGANAKLSSESLLNEARRLQGLDDS
                     GLFFVKDGDTAAAFGTAAKVMEAEYTTNINIHAPLEPMNATAQLQGDIWHIYSGNQFA
                     TRSGGIAAGAAGVDPKFVVMHQMWLGGGFGRRLDADMMVPAVQAAKAVGKPVKVIYSR
                     ENDMTMDYSRPLTFQKVKAGVDGDGKLIAMNHDVVSAWPTKRWGIPDFLSPSVDKKGP
                     LDAFTVNGADFFYSVPNHNVRAILNEMAHNATPSGQLRSVAPGWTFWAVESMVDEVAH
                     ALGKDPAQYRIDMLDGKGANAGGAQRLRNTLLAAMGMAGYGTKQLPKGEGMGVACVSS
                     QERATASWTACVAHVAVAPSGEVKVRKLTVATDVGTQ"
BASE COUNT         1215 a         1996 c         2035 g         1217 t
ORIGIN      
        1 atgcgcgagc cgcgagacct ttcggctcgc attgttcttg tgaatgatgt tgcgctgcgc
       61 tgcctgcatg agttccggtt ccgcacgcgt catcgccttt agcgccgcat cgcggtcgcc
      121 gctcttgatc gcctcttcga cgatgcgaac cgcgccgcgc atctgggtac gacgggactt
      181 gttgatagtg gtgcgtcggg caatcttgcg cgtcgccttc ttggcggagg tcgtattggc
      241 catgctttca aatatccttc gacggtgacc gatcgcgggt gctcaggctt gagcgttccg
      301 cccgttcaac tgcgtgatcg acgctggtat gtttccgggt gttccacgag tatgccattc
      361 tcggagcata gatcgctcac ccgacggcgc cgctcaaaga acagcggcgg caggattgca
      421 tccgccgcca ttggcggtct tatagagggc gcaaattgca gcgtcaacgc tttccgggcc
      481 cccaaagccc ggaagcctga gcggaaggcg ctgataagat gctgatgagg ttgaatttcc
      541 cgcgctgggc cattaatggc tgactgcggt ttcgggcgcg agagacataa ggtgacgcat
      601 gatccgcggt tttttccgac ttgtggggct gctgctgctg gccggcggat ttatcttcat
      661 ggtctatgac ggcgcgcgct gggtcgccga tcagaccttg cgttttaccc gttttggcga
      721 attctggaac gatatccatc aatccagcca gcaggcgttc cgggcctggg tggaggccac
      781 cgcgccctgg ctctggacca gcgtcaaaat tatcctggac caaccggttt ttgccgtgat
      841 gggcgtgttg ggtatcttgc tgatgctgct gttccggccg cgcaagccgc tgatcggcta
      901 ttcgcggaac tgaccgggat ggggaggtgc cggccttatg ttcggctgcc tggcgcaact
      961 gggctgccgt gacccgcgta gatacgtata atagagtgat gtctgttgcc gccgggagat
     1021 gacccatgtt gttcatgcgc aagaccaccg cattgcccag cgcagccgag gcgctgccgg
     1081 gccgcgccaa gccgatcccc accgctacca gccatttcgt caacggccgc ccgttgcagc
     1141 caccttatcc cgccggtctg gagcaggccg tgttcgggct cggctgcttc tggagtgcgg
     1201 agcgtaaatt ctggcagctc ggcgacggta tctacaccac cgcggtgggt tacgccggcg
     1261 gtcataccca aaaccccagt tacgaggagg tttgttcggg cctcaccggt cataccgaag
     1321 cggtgcgggt ggtgttcgat ccgaacaagg tctcgtacga tcaattgctg aagacgtttt
     1381 gggaaagtca caacccgacg cagggcatgc ggcagggcta tgacgtcggc acccagtatc
     1441 gatccgcgat ctacaccttc aatgacgcgc aacgcaaagc cgcggatgca tccaagtcgg
     1501 cctatcaaag ggcgttggcg gccaagggcc tcggcgccat caccacggag atcgcgcctt
     1561 caggcgaatt ctatttcgcc gaggattacc atcagcagta tctggcgaag aatccgaccg
     1621 gctattgcgg gcttggcggc accggggtgt cgtgtccaat cggcgcgggc gtcagcgcct
     1681 gatcgccatc aagcgagcat atcgagattc cgggttcgcg ctgacgcgcg ccccggaatg
     1741 acgaccctga gattacttca aatgcctggc gaagaagccg aggctgcgcg gccaggcgat
     1801 atcggcactg gtcttgtcgt aactcgcccg ctcgtcgcaa tgaaaaccgt gctgcgcccc
     1861 cggatagacg tggatctcga cctcgggccg tcttgcgctg atggtctcga catcggcgag
     1921 cggaatgccc gcatcctttt ctccgaagtg caactgcgtc ggtacgctcg gtttgtcgtc
     1981 ggcgaaacga acgatggcgc cgccgtaata gccgaccgca gccgacagcc ccgacagttt
     2041 tgtcgcggcg gcataagcga tgctgccgcc gaggcagaaa ccgatgatgc cgaccgggcc
     2101 gacatggctg accgcgtcga tgccggcctg ggtatcgcgc agcatcgccg cccagtccgg
     2161 attggcgacg aacttgcgcg ccaccgcgat ctcatcgggc gaatagccgg aggtgaaatt
     2221 gggctcgacg cggtcgaaga tcgcaggcgc gatggcgaca tagccttcac ctgcgagacg
     2281 atcgcagacc gcgcggatgt gatgattgac gccaaagatt tcctggatca ccacgaccgc
     2341 cgccttgggc gcggtggcgg ggtcggcacg atagccgccc agttgaaaat tgtccgaagc
     2401 cgtcagtttg atgtcttgtc ccacgggtca tccatttgct tgagagttga tcttttgcgg
     2461 agagatttga aagcgagagc caagcgtcag gctgcctgct attgccacat ccagttgtgg
     2521 ccccagtcgc ctttccagcc ggtcagtctg cccttgcgga attgcagata gagccggtct
     2581 ttgtggaaga acaacccgct gccgccgacg ttgcggaagg ccagcaaggt ttcgtcgccc
     2641 gggcggccgc tgatgtagtt caatggcgtc cccagggcgc gcgcggcatc ttccgcgtcc
     2701 atcccgaaca cgagcggcgt attgttcgac agcgtcgcag tgaagggtgg ggcattcgaa
     2761 ccacccgccg gcacgaatgt ctgggctgcc gcggcgtcaa tgcctgcggc tccaaggccc
     2821 acgacgaaag ccgcgatcag cgcgagcctg ttcacgacgg gaccatcctt tcggcgacgg
     2881 cgcttcgtgg caggctatcg aggaacggta acaccgcgtc gatgaacgcc tgcggctggt
     2941 cgatgttgac ggcgtggccg gctgccggga tcaccgcctt tttcgcaccc ggaatcttcg
     3001 ccgccatata gtccgcggcc gcgaggaacg gcgtgtcgtc ggcgccaacc accaccagcg
     3061 caggcacctt gatgtcgggc aaggattcga tcacgcgggc atcgcgctgg gtcagcatgc
     3121 cgcgcgcgga gcgcgccagg cccgacgcat cgcgatggct caccatcgag cgctcgcggc
     3181 cgctggattt caagacctca aggtcctcgc gctcgaagcg gtcgccggta tcgagcgcgc
     3241 gtttgttcca ggcctcgcgg gcgtcgtcct tcttgaagcc cggtccggta tcgatgatca
     3301 acagcgcccg cacgcgctcc ggatgcgcgc gataaaacgc cagcgacatg tagcccccga
     3361 gtgaaagccc gccgacgatg gcgctgatgg ccccgacctc gtcgaggagg gctgcgatgt
     3421 cggcaaccgt cagcgcttcg ctataggccg acgcctcatc ggggtaatcg gaccggccgt
     3481 ggccgcgcat gtcccacaac acgagtttgt ggtgtctcga taaggcctcg atctggccct
     3541 gccacatctg cgatgtcgac gaatagccgt gggtcagcag cagcggcggt ccgctgccgt
     3601 ggacctcata atagatctgg accccgtcgc ggttgatttt tggcattgca gtttcctggc
     3661 gattccccgg cggcttcgta agactaccgc gcaacggtat tctagcgcga tccgttcgtc
     3721 tctgggaacc gcccaacttc gcaagcatca atcgccggct ccgcatggcg tgggagcaat
     3781 tccatcgccc attttccgac ggggtctgtt gccgcagcgc acaaagcgca tttcggatca
     3841 ttttctattc gaccaattcc aatttgcatt gcctctggat tggaacgcga tcattctccc
     3901 gcgcaaggtc ggcatcggcc cggtgggtgc cagatcattc gtcaaacgtg aatcgccgcc
     3961 gtaagcggca tcatgcaccg gcaggggaag agaccatgcc tactctcaac atcaatgggc
     4021 ggaatatgtc cgtcgaggcg gcgaacgata cgccgttgct ttgggccatc cgcgaacaat
     4081 tgcagatgac cggtacgaaa ttcggctgcg gcgccggcct ctgtggcgcc tgcacggttc
     4141 acgtcaatgg cgaagcggtg cgatcctgcc agactccgct cagcgacgtc gccggcaaga
     4201 aaatcaccac catcgaaggg ctcagcgcca agggcgacca tccgctgcag aaggcgtgga
     4261 tcgccgagca ggttccgcaa tgcggctact gtcaatccgg acagatcatg caagcggccg
     4321 ccttgctctc caagaacgcc aatccgacca aggacgaggt ggttgcacat atggacggca
     4381 atctctgccg ctgcatgacc tattcgcgca tccaaaaggc gatcatgcgt gcggcctcgg
     4441 aaatgcgcac cgcgtccact tctaccgagc ggagggcaac atgaatacgc acgtgaaaat
     4501 caccaaggat atcgtcgacc gcgagcccgc cgatctcagc cgccgctcgt tcctcgtggg
     4561 atccgccgcc gcaggtcttg cgctcggtta ttccgcggtt ccgggcctcg tcggcgccga
     4621 ccaggcgttc gccgctgcga gcaatttcga tcccagtgtc tggtattcga tcgcgccgga
     4681 cggaattgtc acagtcacct gcggcaaggc cgacatgggc cagcatattg cctcgaccat
     4741 ggcgcagatc atttccgaag agctcggctc gaactggaag gacatgcggg ttcagctcgc
     4801 atccaacgat ccgaagttca acgaccccgt gctcggcgcg cagatcaccg gcggcagctg
     4861 gagcaccatg atgaacttcg atgcgatgag ccgcgcggga gccgcggggc ggatggcatt
     4921 gacggaagca gcaagcgccg cgatgggcgt accggcgggc gaactggtgg tcagggactc
     4981 cgtgatttcg catccgaagt cgaagaagtc gatgaccttt gccgatgtcg tcaagagcgg
     5041 gaaagcgaca aaaaccttta cgcccgacga actcaaggcg atccagctca agacgcctga
     5101 tcagtacacc aagatcggcg tttcggttcc gcagcttgat atcccgtcga agaccaacgg
     5161 gaccgccaaa tacggcatcg acgtcatgct gccgggcatg gtctacggca gggtcgtcac
     5221 gccgccggtg cgctttggcg cgacggtgaa atcggtcgat gatagtgctg cgaagaaggt
     5281 gcctggcttc atcaaggcgg tcaccctcga cgaccagacc ggaagcacga ccggctgggt
     5341 ggtggcggtg gccaacacct attccaacgc catcaaggcg gcggatgcgc tcaagatcac
     5401 ctatgacggt ggtgcgaacg ccaagctgtc gagcgagtcg ctgctcaacg aggcgaggcg
     5461 gttgcagggc cttgacgatt ccggcctgtt tttcgtcaag gacggcgata cggccgcagc
     5521 cttcggcacc gcggccaagg tgatggaggc ggagtacacc accaacatca acatccacgc
     5581 gccgctggag ccgatgaacg ccaccgcaca gctgcagggc gacatctggc atatctattc
     5641 cggcaatcag ttcgcgacac gctccggcgg gatcgcggcg ggtgctgccg gggtcgatcc
     5701 caaattcgtc gtgatgcacc agatgtggct gggcggaggt tttggccgcc gtctcgacgc
     5761 cgacatgatg gtgcccgcgg tgcaggcggc gaaggccgtc ggcaaaccgg tcaaggtgat
     5821 ctactcgcgc gaaaacgaca tgaccatgga ctactcgcgt ccgctgacct tccagaaggt
     5881 caaggcgggc gtcgacggcg acggcaagct gatcgcgatg aatcacgacg tggtcagcgc
     5941 atggccgacc aagcggtggg gaattcccga tttcctgtcg ccgtcggtcg acaagaaagg
     6001 gccgctcgat gcgttcaccg tgaatggcgc ggacttcttc tattccgtgc ccaatcacaa
     6061 cgtccgcgcc atcctcaacg agatggcgca caacgccacc ccgtcgggtc agctgcggtc
     6121 ggtcgcgcca ggctggacct tctgggccgt cgaaagcatg gtcgacgaag ttgcgcacgc
     6181 gctcggcaag gatccggcgc agtaccgtat cgacatgctc gacggcaagg gcgccaatgc
     6241 cggtggtgcg caacgtctgc gcaacacctt gctcgccgcg atgggcatgg ccggctacgg
     6301 caccaagcaa cttccgaagg gtgaaggcat gggcgtcgcc tgcgtctcgt cgcaggaacg
     6361 ggcgaccgca agctggaccg cctgcgtggc ccatgttgcc gtcgcaccga gcggcgaggt
     6421 caaggtcagg aaattgaccg tggccaccga cgtcggcacg cag
//
LOCUS       DASWGM010000402        10881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1706712,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000402 DASWGM010000000
VERSION     DASWGM010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706712"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGA15_11740"
     CDS             complement(<1..384)
                     /locus_tag="VGA15_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9588398.1"
                     /translation="MTIKVGDKLPEAKFRVMTAEGPQVKSTDDIFNGKKVALFAVPGA
                     YTGTCHKMHLPSIFLNAYAIKDKGVNTIAIVSVNDAFVMNAWKRDTDQRDEATFLADG
                     NAEFTKAIGMELDASGNGLGIRSKRY"
     gene            complement(493..1047)
                     /locus_tag="VGA15_11745"
     CDS             complement(493..1047)
                     /locus_tag="VGA15_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX9588399.1"
                     /translation="MTGACGIAPADILAFWRDAGCERWYKRDDAFDAEVRSRFLELWQ
                     KAAAGELSSWEATDDGALALAIVLDQFPRNMFRDDIGAYSSDGLARAVADGAIARGAD
                     IGVEPGLLEFLYMPLMHSEQLADQQRCVELFRKAGSADNLKYAEDHAEIIRRFGRFPH
                     RNAILGRATTMPEQAFLDSGGFSG"
     gene            complement(1074..2756)
                     /locus_tag="VGA15_11750"
     CDS             complement(1074..2756)
                     /locus_tag="VGA15_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9588400.1"
                     /translation="MERIWLKQYPAGVPADVDVTQYTSLVELLEESFAKFSDRKAFIC
                     MDKSISYRDLDEMSLALAAYLQSKGLQKGARVALMMPNVLQYPVSTAAVLRAGYTVVN
                     VNPLYTPRELEHQLKDSGAEAIIVLENFATTVQQVMARTAVKHVIVGSMGDLLGLKGM
                     IVNLVVRRVKKMVPAYSIPGAVAFNDAVVAGRSVKLNKPKLAPDDVAFLQYTGGTTGV
                     SKGATLLHRNILANVLQNDAWLQPALRKPPHVDQLLIVCALPLYHIFALTACFLLAMR
                     AGGVNLLIPNPRDMAGFIKELMKYQVNSFPAVNTLYNGLLHSPGFDKVDFSKLKTSFG
                     GGMATQKAVAEAWLKTTGCALSEGYGLSETSPTLTCNPADTDKFSGSIGLPVPSTYLS
                     IRDDDGNEVPLGQPGEICAKGPQVMAGYWNRPEETANVTTADGYFRTGDIGVMTPDGY
                     TKIVDRKKDMILVSGFNVYPNEIEEVIASHPGVLECAVIGVVDPRSGEAVKAFVVKKD
                     PNVTAEDIIKFCGTQLTNYKVPKQIEFRTSLPKTNVGKILRRELRDEKKAAA"
     gene            complement(2907..4238)
                     /locus_tag="VGA15_11755"
     CDS             complement(2907..4238)
                     /locus_tag="VGA15_11755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX9588401.1"
                     /translation="MVLLAALAIVVPRVVQAEALLVVEADTGKVLQADNATYPWYPAS
                     VTKLMTAYVTLKAVKDGHITLDTLFTVSPVAASQSPSKMGFRPGTQVTVDNALKMMLV
                     KSANDMAVVLAEGVGGSIDGFSAQMNEAAQRLGMTQSSYVNPNGLPADGQITSARDLA
                     ILARAIIRDLPEYEYFVHIPSIRFGRKVTQNFNKLIGRYPGADGFKTGFICASGYNLV
                     ASATRNGKRLIAVVLGASSGQMRAVRAAQLLERGFANNQLAWLRPSLGTVDNLVPIDA
                     SPPNLRDEMCGGKHKRPASDEDEDTVASNASGPSTGETAITFFTAGLQPPMAKPSDLL
                     AAEAAPSEPIPVYTGPTKTGPALIAAVAMDTEMQTPPRRGKKSRVAAKKPNTAAEPAA
                     ARHANAKADGTPKVAEKPAKPKAVAKPKPAPKSTTSEANPVDQKTATAPHT"
     gene            complement(4496..6301)
                     /locus_tag="VGA15_11760"
     CDS             complement(4496..6301)
                     /locus_tag="VGA15_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HEX9588402.1"
                     /translation="MSLLRLYTRVLHLLGKEARLGWILAGANLLLAGAQFAEPVLFGR
                     IVDVLSGNPSAGPLASSSAWPLLGAWAAFGLFTIGCSAAVALHADRLAHRQRQAVLTN
                     YFEHIMQLPLTFHTGTHSGRLMKVMLNGTDSLWRLWLAFFREHFAAIMSLVVLLPLAL
                     YINWRLAILLFALCVVFTVLTTLVVRKTYGMQSEVEAHYSDLSARASDALGNVALVQS
                     FVRIDAEVQGLRFVADKLLAVQMPVLSWWALVTVITRASTTITVLAIFTVGIALHDQG
                     LTSVGEIVMFVSFATMLIQKLEQVVSFINNVFMEAPRLQEFFHVLDAVPAVRDRPDAI
                     DAGRLSGLVEFNDVSFSYDGKRPAVEDLSFTALPGQTVALVGPTGAGKSTAIALLHRA
                     FDPQSGFIRIDGMDVRGLTLTALRRNIGVVFQEALLFNRSIADNLRVGKPDATDEEMR
                     IAAARAQALEFIERSERGFESNAGERGRMLSGGERQRLSIARALLKDPPILILDEATS
                     ALDAVTEAKLNAALDEVMRGRTTFVIAHRLSTIRNATRILVFDSGRVIESGTFDELVA
                     KGGRFAELARAQFMVQETARASIQAKGTDSAAVKI"
     gene            complement(6399..7457)
                     /locus_tag="VGA15_11765"
     CDS             complement(6399..7457)
                     /locus_tag="VGA15_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588403.1"
                     /translation="MRVNQTNAARCFLASLTGLLVLAIFGLAASRAGSDVRQPVAVQF
                     SLDRPIDASAAPFVMAAARGMFGSEGLAVTTNIASGSQDAIARVAAGTSDFALVDINA
                     LIRFHDRPNAAPIKAVFVLFNQSPYAIVARKNRGIHALSDIEGKNLGVGEGDLSIRLW
                     PALARQNGIKIASVKQNSISAAVREPMLSAGQVDAVTGFSYLSGVNLRDRGVPAEDLA
                     VLRFADYGCEAYGFSVIVNPAFAAAKPEAVKGFLRSVIAGTHLAIKDPGRAADEVVSR
                     MDDGSRDLELERLDTVIRDNILTSEVKRDGIGGIDPARLERSIDQIAEDFKFRKRPSA
                     KDIFDDAFLPPLDGRLIN"
     gene            7670..8077
                     /locus_tag="VGA15_11770"
     CDS             7670..8077
                     /locus_tag="VGA15_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588404.1"
                     /translation="MFARKALFSSLGRAAVMATVAAVALTAVEPSLARAGSAAAGKGV
                     SAAAATSGATDFSARRRYYRGGGGAAAAAAFAGIVGTGLAIAASQNRRDDYYYDRGPA
                     YYGGGPYYGGNPYYGGYGYHRDPYFTGYPNAGW"
     gene            8204..8605
                     /gene="hisE"
                     /locus_tag="VGA15_11775"
     CDS             8204..8605
                     /gene="hisE"
                     /locus_tag="VGA15_11775"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX9588405.1"
                     /translation="MSDSLERLYLAVIAAKDLDPATSRTARLFQRGPTKMAKKLAEEA
                     IEVVIDAVNGNTPAVIRESADLLYNLTVLWASAGVRPEDVWREMERREHLLGIAEKLP
                     KSVVKLPKAAPAPAVRRRIVALEGRSLRKRH"
     gene            8649..9248
                     /locus_tag="VGA15_11780"
     CDS             8649..9248
                     /locus_tag="VGA15_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX9588406.1"
                     /translation="MVVHRAMLRRIYDWCIDAAHKPYALWILGAVAFAESSFFPVPPD
                     VMLIPMSLARPERAWLYASVCTAASVLGGILGYAIGALLYDSIGHWLIQFYGLGGKVE
                     AFRAGYAEYGAWIILLKGLTPIPYKLVTITSGFANYNIWLFVALSVIARGGRFFVVAI
                     LLNRYGEWIRVRIEKHLGLWVALAAGVLVLGFIVAFRML"
     gene            9375..9899
                     /locus_tag="VGA15_11785"
     CDS             9375..9899
                     /locus_tag="VGA15_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588407.1"
                     /translation="MLMIAAGVGWPQTPPSSLGIQTPAPQAAPDRPAPLQGEPAPSSA
                     PPEREDNPGLINEMGKLFEKTLSILPPLKSPQETIDDLNSRAKDAGDGLTGLAKPSSM
                     VTGRMGCPVAANGAPNCKAGADKLCQAKGFKEGKSLDTDAAEKCSAKVYLPGYKRGPG
                     DCRTENYVTRALCQ"
     gene            10034..>10881
                     /locus_tag="VGA15_11790"
     CDS             10034..>10881
                     /locus_tag="VGA15_11790"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX9588408.1"
                     /translation="MSMPGLFKGRLSLPVIGAPLFIISVPDLVIAQCKAGVVGSFPSL
                     NARPPALLDEWLVRIKEELAAHDKAHPDRLSAPFAVNQIVHRSNNRLDQDLALCEKHK
                     VPMVITSLGAREELNQAAHRWGGIVFHDVINQKFAHKAIEKGADGLILVSAGAGGHAG
                     EISPLAFVAETRQWFDGPIALSGAIANGRAIRAARILGADFAYVGSAFIATAEANAVE
                     GYKQMITTSSAEDIVYSNLFTGVHGNYLKPSIVAAGMDPDNLPESDVSKMSFGTDASG
                     ERAKPKA"
BASE COUNT         1961 a         3336 c         3465 g         2119 t
ORIGIN      
        1 atagcgcttg gagcggatgc cgaggccgtt gccggaggcg tcgagctcca tgccgatcgc
       61 cttggtgaat tcggcgttgc cgtcggcgag gaaggtggct tcgtcgcgct ggtcggtgtc
      121 gcgcttccac gcgttcatga cgaaggcgtc attgaccgag acgatcgcga tcgtgttcac
      181 gcccttgtcc ttgatcgcgt aggcgttgag aaaaatgctc ggcaggtgca tcttgtggca
      241 ggtgccggtg taggcgccgg gcaccgcgaa cagcgccacc ttcttgccgt tgaaaatatc
      301 atcggtggat ttgacctgcg gaccttccgc cgtcatcacg cgaaattttg cctcgggcag
      361 cttgtcgccg actttgatgg tcatcttcaa ttctccttga ctgtgcggga atgtcttaaa
      421 ccgggcgggc cgcaggcaca atattcggcg ccacggaaac ggcaatggcc ttgggcgttc
      481 accgttctgt catcagcccg aaaatccgcc gctatcgaga aaggcctgtt ccggcatcgt
      541 cgtcgcgcgc cccagaatag cattgcgatg cgggaaacgc ccgaaccggc ggatgatctc
      601 ggcatgatcc tcggcgtatt tcaggttgtc cgcactgccg gccttgcgga acagttcgac
      661 acaccgctgc tggtcggcca gctgctccga atgcatcaaa ggcatgtaga gaaattccag
      721 caaccccggc tcaacgccga tgtcggcgcc gcgtgcaatg gcgccgtccg cgaccgcgcg
      781 ggccagccca tcgctcgaat aggccccgat atcgtcgcga aacatgttgc gcggaaactg
      841 atcgagcacg atcgccagcg ccagcgcgcc gtcgtcggtt gcttcccagg acgacagctc
      901 gccggcagcg gccttctgcc acagctcgag gaagcggctg cgcacctcgg cgtcgaacgc
      961 gtcgtctcgc ttgtaccagc gctcgcagcc agcgtcgcgc cagaatgcga ggatgtcggc
     1021 cggcgcgatg ccgcaagcgc cagtcatgaa cagccggccg cgatcatctt cgttcaggcg
     1081 gcggccttct tttcatcgcg caattcgcgg cgcaggatct ttccgacatt ggtcttgggc
     1141 aggctggtcc gaaactcgat ctgctttgga accttgtagt tggtcaattg cgtgccgcag
     1201 aacttgatga tgtcttcggc ggtgacgttg gggtccttct tgacgacgaa cgccttgacg
     1261 gcttcgccag accgcgggtc gacgacgccg atcacggcgc actccagcac gccggggtga
     1321 ctggcgatga cctcctcgat ctcgttggga tagacgttga agcccgacac caaaatcata
     1381 tccttcttgc gatcgacgat cttggtgtag ccgtccggcg tcatcacgcc gatgtcgccg
     1441 gtgcggaagt agccgtccgc ggtcgtcaca ttcgcggtct cttccggcct gttccagtag
     1501 cccgccatca cctgagggcc cttggcgcag atttcgccgg gctggccgag cggcacctcg
     1561 ttgccgtcgt cgtcgcggat cgagaggtag gtcgatggaa cgggcaggcc gatcgagccg
     1621 gagaacttgt cggtatcggc cgggttgcag gtcaaggtcg gcgaggtttc cgacaggccg
     1681 taaccttccg acagcgcaca gccagttgtc ttcagccagg cttccgcgac ggccttctgc
     1741 gttgccatgc cgccgccgaa cgaggttttc agcttggaga agtcgacctt gtcgaagccg
     1801 ggcgaatgca acagcccgtt gtagagcgtg ttgaccgcgg ggaaactgtt gacctggtat
     1861 ttcatcagct ccttgatgaa gccggccatg tcgcgcgggt tgggaatcaa gagattgacg
     1921 ccgccggcgc gcatcgccag caggaaacag gccgtcagcg cgaagatgtg atagagcggc
     1981 agcgcgcaga cgatgagtaa ttggtcgaca tgcggcggct tcctcagcgc gggctgcagc
     2041 caggcatcgt tctgcagcac gttggccaga atattgcgat gaagcagcgt tgcgcctttg
     2101 gagacgccgg tggtgccgcc ggtgtattgc aggaaggcga catcgtctgg cgcgagcttc
     2161 ggcttgttca gctttacgct gcggccggcc acgaccgcgt cgttgaaggc gacggcgccc
     2221 ggaattgaat acgccggcac cattttcttg acgcggcgta ccaccagatt gacgatcatg
     2281 cccttcaggc cgagcagatc gcccatgctg ccgacgatga cgtgtttcac cgcggttcta
     2341 gccatcacct gctgcacggt ggtcgcgaaa ttctccagaa cgataatcgc ctcggcgccg
     2401 gaatccttga gctggtgttc aagctcgcgc ggggtgtaga gtggattgac gttcacgacc
     2461 gtatagccgg cgcgcagcac ggcggcggtc gataccggat actgcagcac gttcggcatc
     2521 atcagcgcga cgcgggcgcc cttttgcagg cccttgctct gcagataggc cgcgagcgcc
     2581 agcgacatct cgtcgagatc gcgatagctg atcgacttgt ccatgcagat gaaggccttg
     2641 cggtcgctga acttcgcaaa gctttcttcc agcaactcga cgagagatgt gtattgggtc
     2701 acgtcgacat cggcgggcac gccggccgga tattgcttga gccagatccg ctccatgata
     2761 tttccccttc cggtttgctt aacccgtttt gggacgggag gccttgtttt gggcagtatt
     2821 gagcaatgtg gcggcccaag gcaagcggct gcgctgtcgg catatcgtta actaagacgg
     2881 ggccgtccga gcgaccgaaa tccagctcag gtgtgcggtg cggtggcggt cttttgatcc
     2941 accggattgg cctcgctggt cgtgctcttc ggcgcaggct tgggcttggc gacagccttg
     3001 ggctttgccg gcttttccgc gacctttgga gtgccatccg ccttggcatt cgcgtgtctc
     3061 gcggcggccg gttcggccgc cgtattgggc tttttcgccg ccacccgcga cttcttcccg
     3121 cgacgtggcg gcgtctgcat ttcggtgtcc atggccacgg cggcgatcag ggcaggcccg
     3181 gtcttggtcg gaccggtata gaccgggatc ggctccgagg gcgcggcctc cgccgccagc
     3241 aggtccgagg gcttcgccat cggcggctgc agcccggcgg tgaaaaacgt gatggcagtt
     3301 tcgccggtgc ttggaccgct ggcattgctg gcgacggtgt cttcatcctc gtcgctggct
     3361 gggcgtttgt gttttccccc gcacatctcg tcgcgcaggt taggcggcga ggcgtcgatc
     3421 ggcaccagat tgtcgacggt gccgagcgag ggccgcaacc acgccagctg gttattggca
     3481 aaaccgcgct cgagcagttg ggcggcgcgc accgcgcgca tctggccgga tgacgcgccg
     3541 agcaccaccg cgatcagccg tttgccgttg cgcgtcgccg acgccaccag attgtagccg
     3601 gaagcacaga tgaagccggt cttgaagcca tcggcgccgg ggtagcgccc aatcagcttg
     3661 ttgaaattct gcgtcacctt gcggccgaag cggatcgagg gaatgtggac gaaatactcg
     3721 tattccggca ggtcgcgaat gatcgcgcgg gcgaggatcg ccagatcgcg cgccgaagtg
     3781 atctgaccgt ccgccggcag tccgttcgga ttgacgtagc tcgactgcgt catgcccagc
     3841 cgctgcgcgg cctcattcat ctgagccgaa aagccgtcga tcgaaccgcc gacgccttca
     3901 gccagcacca cggccatgtc gttggccgac ttcaccagca tcatcttgag cgcattatcg
     3961 accgtgacct gcgtccccgg ccggaaaccc atcttggatg gcgactgtga ggccgcgacg
     4021 ggcgataccg tgaacagagt atcgagcgtg atgtgtccat ccttgacggc cttgagcgtg
     4081 acataggccg tcatcagctt ggtcaccgat gcgggatacc agggataggt cgcattgtcg
     4141 gcctgcagca ccttgccggt atcggcctcg acgaccagca gtgcttccgc ctgcacgacg
     4201 cgtggaacaa cgatagcgag cgctgcgaga agaacgatcc agcacgacga tgacttgcaa
     4261 ggcaacgggc gaggaagatg cactatccga ttccggtcct ttgagaccag cctttgaaga
     4321 ggaggtctta tgagaggagg ccttacaaag aggaagcctt ggcgtgtgat gttcgggttt
     4381 caagcctgtc cggcgccgcc ggttgtggcg ggcgcgaatc tataccggct tatcggtcca
     4441 gaacagaggc tgtgcgatta aatcgtggac gaaatgggct gaatttcggc gatccctaaa
     4501 tcttgacagc cgcgctgtcg gttcccttcg cctgaatgct ggcccgcgcg gtctcctgta
     4561 ccatgaactg agcccttgcg agctccgcaa aacgaccgcc tttcgcgacc agttcatcga
     4621 aagttccgct ctcgatgacg cgtccactat cgaacaccag aatccgtgta gcgttgcgaa
     4681 tggtcgacag ccggtgcgcg atcacgaaag tcgtgcgtcc cctcatcact tcgtcgaggg
     4741 ctgcgttaag cttggcttcg gtgaccgcat cgagcgcgct ggtcgcctca tcgaggatca
     4801 ggatcggggg atccttcaac agcgcgcggg cgatcgacaa ccgctggcgc tcgccgccgg
     4861 acagcatgcg tccgcgctcg ccggcattgc tctcgaagcc ccgctcgctg cgctcgatga
     4921 attcgagcgc ctgggcccgg gcggcggcga tccgcatttc ctcgtcggtg gcgtccggct
     4981 tgccgacgcg caaattgtcc gcgatcgaac ggttgaacag caaagcctcc tgaaacacca
     5041 cgccgatatt ccgccgcagc gccgtcagcg tcaggccgcg gacatccatg ccgtcgatcc
     5101 tgatgaagcc cgactgcgga tcgaacgcgc ggtgcagcaa cgcgatggcc gtcgacttgc
     5161 cggctcctgt cggaccgacc agcgcgacgg tctggccggg cagcgcggtg aaggaaaggt
     5221 cttcgaccgc cggccgcttg ccgtcatacg aaaacgagac gtcgttgaat tccaccaggc
     5281 ccgagagtcg tccggcgtcg atcgcgtcgg gccggtcgcg gaccgcgggc acggcatcca
     5341 gcacatggaa gaattcctgc agccgcggcg cctccatgaa cacattgttg atgaagctga
     5401 ccacctgttc gagcttctgg atcagcatgg tcgcgaaact cacgaacatc acgatctcgc
     5461 cgactgacgt aaggccctga tcgtgcagcg cgatgccgac ggtgaagatc gccagcacgg
     5521 tgatggtggt cgacgcccgg gtgatgacgg tgaccaacgc ccaccacgac agcaccggca
     5581 tctgcaccgc caatagcttg tcggcgacga agcgcaaccc ttgcacctcg gcatcgatgc
     5641 gcacgaagct ttgcaccagc gcgacattgc cgagcgcatc cgaagcgcgg gcggacagat
     5701 cgctgtaatg cgcctcgacc tcgctttgca tgccataggt cttgcgcacc acgagcgtgg
     5761 tcagcaccgt gaataccacg cacagggcga acagcaggat cgccagccgc cagttgatgt
     5821 aaagcgccag cggcagcagc accaccagcg acatgatcgc ggcgaaatgc tcgcggaaaa
     5881 atgccagcca cagccgccac agcgagtcgg tgccgttcaa catcaccttc atcaggcggc
     5941 cggaatgggt gccggtgtgg aaggtcagcg gcaactgcat gatgtgctcg aaataattcg
     6001 tcagcacggc ctgacgctgt cggtgcgcca gccggtcggc atgcagtgcg accgcagcgc
     6061 tgcagccgat ggtaaacagg ccgaacgccg cccaggcccc cagcagcggc cacgcggacg
     6121 acgaagccag cggacccgca gacgggttgc cggaaagaac atcgacgatg cggccgaaca
     6181 gcaccggctc ggcgaactgc gcgccggcca gcagcagatt ggcgccggcg agaatccagc
     6241 ccagccgtgc ctctttgccc agcaaatgaa ggacgcgcgt gtaaaggcgg agcagggaca
     6301 tgcgggggga ctcaaggggt cgcagcgggc cgttgatcgc tcgggcgtgc attctagtca
     6361 gggatcgccg ggagatacag cgtcagctga gtttttgctc aattgatcag ccggccgtcc
     6421 aacggaggca ggaaagcatc gtcgaaaata tccttcgcgg atggccgctt ccggaatttg
     6481 aaatcctcgg cgatttgatc gatcgaccgc tccaggcggg ccggatcgat gccgccgatg
     6541 ccgtcgcgct tcacttcgct ggtgaggatg ttgtcgcgga taacggtatc caggcgttcc
     6601 agctcgagat cgcgcgatcc gtcgtccatc cggctgacaa cctcatccgc cgcgcgcccg
     6661 gggtccttga tggccagatg ggtgccggca atcaccgagc gcaggaagcc tttcaccgcc
     6721 tcgggcttgg cggccgcgaa cgccggattg acgatcacgg aaaacccgta ggcttcgcag
     6781 ccatagtcgg caaagcgcag caccgccaga tcttcagccg gaacgccgcg atccctcaaa
     6841 ttgaccccgg acagatatga aaacccggtc acggcatcga cctgacccgc ggacagcatc
     6901 ggttcgcgta cggcggcgct gatgctgttt tgcttcacgc ttgcgatctt gattccgttc
     6961 tgccgcgcca gcgccggcca cagccggatc gaaaggtcgc cttcgccaac gccgaggttc
     7021 ttaccttcga tatcggagag cgcgtggatg ccgcggttct tgcgggcgac gatggcatag
     7081 ggcgattggt tgaacagcac gaataccgct ttgatgggcg cagcgttcgg cctgtcgtga
     7141 aagcggatca gcgcgttgat gtcgaccagc gcgaaatcgc tggtgcccgc ggcgacgcgc
     7201 gcgatcgcgt cctgcgagcc gctggcgata ttggtggtga ccgcgagacc ctccgagccg
     7261 aacatgcctc tcgccgccgc catgacgaac ggggccgcac tcgcatcgat cggtcggtcg
     7321 agggaaaact ggactgcgac cggttgccga acgtcgcttc ccgcccggct cgctgccagg
     7381 ccgaaaattg ctagaaccag taggcctgtc agcgaagcca aaaagcagcg ggctgcgttg
     7441 gtctgattta cgcgcattga gactacatcg cgaccggaca gcagttgcgc cggctatgtc
     7501 ggtttcatgg cgcctggggc gccgggggca ggcattctgc cggatgatat ctgaacggtc
     7561 gatgaccacc acgatttagc cgctcgcatc cggatcgggg aacctaatgt gggctcgctg
     7621 gttggctccg ggaggcctgc gaggccaggc cgtcatggag gattttgaga tgttcgcgcg
     7681 aaaagctttg ttttcatccc ttggccgcgc cgccgtgatg gcgaccgtag cggcggtggc
     7741 gctgaccgca gtcgagccat cgctggctcg tgccggctct gcggccgccg gcaagggcgt
     7801 gtcggccgcg gcagcgacca gtggcgccac tgacttcagc gcccggcgtc gctattaccg
     7861 tggtggcggt ggcgccgcag cggcagctgc ttttgccggg attgtcggca ccggtcttgc
     7921 gatcgcggcg tcccagaacc gtcgcgacga ctattattac gatcgcggcc cggcctatta
     7981 cggtgggggc ccctattatg gcggtaaccc ctattatggt ggatatggtt atcaccgcga
     8041 cccgtatttc accggttatc ccaacgccgg ctggtaaccc tgcattttcc tagccgatag
     8101 atccgccggc tggttgggta gccggcggat tttctttccg gtttgcggct tcgggtaacc
     8161 agccgttaac acgctggaca ctggtttgga ctagttttgc gggatgagtg attcgctcga
     8221 acggctttat ctggcggtca tcgcggccaa ggatctcgat ccagccacgt cgcgaaccgc
     8281 gcggctgttt cagcgcggcc cgaccaagat ggccaagaaa ctggccgaag aggccatcga
     8341 agtcgtgatc gatgccgtca atggcaatac ccccgcggtc atccgcgaga gtgccgatct
     8401 gctctacaat ctcaccgttt tatgggcgtc ggccggcgtc cgccccgagg acgtgtggcg
     8461 cgaaatggag cggcgcgaac atctgctcgg aatcgccgag aagttgccga aatccgtggt
     8521 caagctgccc aaagcggccc cggcccccgc cgtccggcga cgaattgtcg cgctcgaagg
     8581 ccgcagcctg cgcaagcgac actaaaccgc cttcaatcgg catttcccgc catggacaaa
     8641 tccgctccat ggtggttcat cgcgccatgc tgagacggat ttacgattgg tgtattgacg
     8701 ccgcgcacaa gccatacgcg ctgtggattt tgggagcggt ggcgttcgcg gaaagttcgt
     8761 tctttccggt gccgccggat gtgatgctga tcccgatgtc gctggcgcgg ccggagcggg
     8821 cgtggctcta tgcctcggtc tgcacggcgg cctcagtgct gggcggcatt ctcggctatg
     8881 cgatcggcgc gctgctgtat gattcgatcg gacattggct gatccagttt tacggtcttg
     8941 gtggcaaggt cgaggccttc cgcgccggat atgccgaata cggtgcgtgg attatcctgc
     9001 tcaagggctt gacgccgatc ccctacaagc tcgtcaccat cacctcgggt tttgccaatt
     9061 acaacatctg gctgttcgta gcattgtcag taatcgcgcg tggcgggcgc tttttcgtgg
     9121 tcgctattct tctcaaccgc tacggcgaat ggatccgggt gaggatcgag aaacatctgg
     9181 ggctttgggt cgcgctggcg gcaggtgtgc tggtgctggg ttttatcgtt gcatttcgaa
     9241 tgctctgaca atccctggtg aggcgctttg ccggtttgct gcttcaggtt acgctcaacg
     9301 tcatgcttgg tcgaaccgtc agccttatca cgctatccag gacgcatgtc gcgaccgcgg
     9361 gcgtggcgtt gtcgatgttg atgatcgccg cgggcgtcgg gtggccgcaa acgccgccgt
     9421 cttcgcttgg catccagacg ccggccccgc aagctgcgcc tgatcggcct gcgcccctgc
     9481 aaggcgagcc tgcgccgtca tccgctccgc cggagcgcga agacaatccc ggcctgatca
     9541 acgaaatggg gaaattgttc gagaaaaccc tgtcgatcct tccacccctg aaaagccctc
     9601 aggaaaccat cgacgatttg aactcgcgcg ccaaggatgc gggcgatggc ctgactggtc
     9661 tggccaaacc atcatcgatg gtgacggggc gcatgggatg tcccgtggcg gccaatggcg
     9721 cgccgaattg caaggccggc gccgacaagc tctgtcaggc caagggcttc aaggagggca
     9781 agagcctcga caccgacgcg gccgagaaat gctcggcaaa ggtgtacctg ccgggctaca
     9841 agcgcgggcc gggcgactgc cggaccgaaa attacgttac ccgcgcgctc tgccagtagc
     9901 gccgggatcg ggccgttggc cggctgggcc tcctcggtac gattgccttg ttggtatgat
     9961 gtctggcatc tttagatcga cgcatcgcgt ttgccacgaa tcttgccctg aatgaccaaa
    10021 aggatttttc ggaatgtcca tgcctggctt gttcaagggc cgtctgtctt tacccgtgat
    10081 cggtgcgccg ctgttcatta tttccgtgcc cgatctggtg atcgctcagt gcaaggcggg
    10141 tgtcgtcgga tcgtttccgt cgctgaacgc gcgtcccccg gcgctgctcg acgagtggct
    10201 ggtgcggatc aaggaagagc ttgccgcgca cgacaaggcg catcccgacc ggctgtcggc
    10261 gccgttcgcg gtgaaccaga tcgtacaccg atcgaacaac cggctcgatc aggatctcgc
    10321 gctttgcgag aagcacaagg tgccgatggt catcacatcg ctcggcgcgc gcgaagaact
    10381 caatcaggcc gcgcatcgct ggggtggcat cgtttttcac gatgtgatca atcagaaatt
    10441 cgcgcacaag gcgatcgaga agggcgcgga cggtctgatc ctggtatcgg ccggcgcggg
    10501 cggccatgcc ggcgagatat cgccgttggc gttcgtggcg gagacccggc aatggttcga
    10561 cgggccgatc gcgctatcgg gcgcgatcgc caacggccgc gccatccggg cagcgcggat
    10621 actcggcgct gactttgctt atgtcggctc ggcatttatc gccaccgccg aagccaacgc
    10681 ggtagagggc tacaagcaga tgatcaccac gtcatcggcg gaagacatcg tctattcaaa
    10741 cctgttcacc ggcgtgcacg gcaattatct gaagccgtcg atcgtcgcgg ccggaatgga
    10801 tcccgacaac cttccggagt ccgacgtgtc gaaaatgagc ttcggcaccg acgcatcggg
    10861 cgagcgcgcc aaaccgaaag c
//
LOCUS       DASWGM010000403         5765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2118661,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000403 DASWGM010000000
VERSION     DASWGM010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2118661"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VGA15_11795"
     CDS             <1..406
                     /locus_tag="VGA15_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006073438.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEX9588409.1"
                     /translation="AVIITNPEVSSVRDSDRIIGMLDSKTVKAEKGEQVKKHVLITRY
                     DTARAARGEMLTIEDILEILATPLLGIIPESQDVLRASNVGSPVTLSNAASAPARAYT
                     DAARRLMGETVTMLVPVERKGLMDRLLGRRAA"
     gene            403..693
                     /gene="minE"
                     /locus_tag="VGA15_11800"
     CDS             403..693
                     /gene="minE"
                     /locus_tag="VGA15_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEX9588410.1"
                     /translation="MSASLLRFFGRRAASASPSAPVARERLQILLAHERSLRGEPDLL
                     VLLRDEILAVVSRHVTLDPDRVIVKMDRRETVSTLEVDIELPNGFERIAAAG"
     gene            801..1289
                     /locus_tag="VGA15_11805"
     CDS             801..1289
                     /locus_tag="VGA15_11805"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9588411.1"
                     /translation="MSPKRPALPMTGGCPCRAIRYQITSFPLLLYTCNCTDCQRASGS
                     AFALNMPVAARDFHILQGEPKGWRHTSPIGVAVISWFCGDCGGRLYGDRAGREAIVNL
                     RAGTLDDTAWLVPVAHMFTRSAQSWVAPAANAECHETGPDDFRPLAQRWRAMWPEFLP
                     QK"
     gene            1348..2847
                     /locus_tag="VGA15_11810"
     CDS             1348..2847
                     /locus_tag="VGA15_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX9588412.1"
                     /translation="MNLVEPITSKAKTGIWGLDDILSGGFSRGHVFLVEGAPGTGKTT
                     IALQFLMEGAQAGEKCLYITLSETERELREGAASHGWSLEDGIEVFELLPPESLLDSE
                     QQQSLLYSSDLELGETTKQIFEAVERARPSRVVLDSLSEIRLLAQSSLRYRRQILAIK
                     HYFAKFGTTVMLLDDLTTELADKTVHSVAHGVLRLEELAPAYGAERRRARIIKYRGVK
                     FRGGYHDATITTGGLNIFPRLVASEYRSSFVRARMSSGIAELDRLLGGGIEAGSSTLI
                     LGPAGTGKSLLAIVFIAAAIARGEKAALFVFDEELGLLFARMKGLGVDLEEMQRSGNL
                     FVEQVDAAELSPGEFAHRVRKRVDEDDIKTVVIDSLNGYQAAMPEENSLILHMHELLQ
                     YLNRRGAATFMTVAQHGLVGDMKAPVDVTYLADTVMLLRYFEALGTVRRAMSIIKKRT
                     GAHESTIREYRIDGRGLTIGDPLDGFQGVLRGVPTYVGEGQPLLEEQVQ"
     gene            2874..4958
                     /locus_tag="VGA15_11815"
     CDS             2874..4958
                     /locus_tag="VGA15_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9588413.1"
                     /translation="MVLAPTGRDASVAAALIREAGYYANVCGDLAAMLREIEGGAGLA
                     VIADEAIKTADLRGLMRWLNDQPSWSDFPIVLLTHQGGGPERNPDAVRLGQVLGNVTF
                     IERPFHPTTLVSVVGSAVRGRRRQYQTRAILQDLTESEGLLQTALSAGRLGALELHLP
                     ELELEASDTCKTFFGRRPGEPFSYQDLLAAVHPDDRARRLDVLDRTIKTGRDYSIEYR
                     NIWPDGSQHWVDVRARAVRRPDGSIKSLVGVSSDITARKVAEIERENLLAQLAAERTA
                     LAELTATLEQRVEQRTADLMKEVAAREKAQEQLRQAQKMETIGQLTGGVAHDFNNLLM
                     VVMGNLDLLRKRIPDDPRLHRLIDGAVLGAERGASLTQRLLAFARQQDLRAVPVDLRT
                     LIQGMSDLLQRSLGPRVVLRLDIPQGLPAARVDANQLELAILNLAINARDAMPDGGSI
                     DIKVVQYQASNDPALKSGGYLKLSVIDTGVGMTAEILKRAVEPFFSSKPLGKGTGLGL
                     SMVHGLAVQLGGTLQLSSTVGNGTTATLILPVAINLPKMESPVHSVQKVNRSAVILFV
                     DDDPLIAMSTTEMLEDLGHHVIGASSGLHALDIIRSEQPIDLMMTDHVMPGMTGIELA
                     AASRLVRPSLPILLATGYAELPEGAQLDLPRLAKPYHQDQLRDRLDQLLGGDEREPLS
                     DHPLAARPEARL"
     gene            complement(4914..>5765)
                     /locus_tag="VGA15_11820"
     CDS             complement(4914..>5765)
                     /locus_tag="VGA15_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9588414.1"
                     /translation="GFDPDAVFKTIERERINFTLFVPTMIYVLLDHPALDRTDLSSLE
                     LLLYGASAMSPSRLVEGIERIGPVFSQLYGQTECYPVSVLRKADHDPKTPELFLSCGF
                     PIAACDVKILDDGDQEVETGEAGEICVRAPHVMAEYWKRPEQTAETLKNGWLHTGDIA
                     RADERGYMFILDRKKDMIVSGGFNIFPREVEDVLSQHADVAMVAVVGVPDDKWGEAVT
                     AVIVVREGARPDAQELIDLVKARKGSAHAPKHIKFVSELPMTGVGKVDKKLLKAGFWA
                     GRERMVG"
BASE COUNT         1043 a         1786 c         1882 g         1054 t
ORIGIN      
        1 ggctgtcatc atcaccaacc ccgaagtctc gtccgtgcgg gactccgacc gcatcatcgg
       61 gatgctcgat tcaaaaaccg tgaaggccga aaagggcgag caggtgaaga agcacgtgct
      121 gatcacccgg tatgatacgg cgcgcgcggc gcggggcgaa atgctcacca tcgaggacat
      181 tcttgaaatc cttgccaccc cgcttctcgg catcatcccc gagagtcagg acgttctgcg
      241 tgcgtcgaac gtcggatctc cggtcactct gagcaacgcc gcgagtgcgc cggcgcgcgc
      301 ctacaccgac gcagcccggc gcctgatggg cgagaccgtc accatgctgg tgccggtcga
      361 gcgcaaaggc ctgatggacc gattgctggg acggagggcc gcatgagcgc cagtctgctg
      421 cggttcttcg gtcgccgcgc tgcgtccgcg tccccttccg cacctgttgc ccgggaacgg
      481 ttgcagattc tcctggcgca tgaacgcagc ttgcgcgggg agcctgactt gctggtgctg
      541 ttgcgggacg aaattcttgc cgtggtgtcg cggcatgtca cgctcgatcc ggaccgggtg
      601 atcgtcaaga tggacaggcg cgaaacggtg tcgacgctcg aggtcgatat cgaactgccc
      661 aacggattcg aaagaatcgc ggctgccggc tagcgatcgg gcgcaccggc ggcgcttcgc
      721 tctgttgccc ggatatcggc gtcgcaacgc gataaattcc atgataatcc ttcgatcgta
      781 gcttcgatcg gaggtcgatc atgagcccga agcgacctgc gcttcccatg accggcggct
      841 gcccgtgccg tgccatccgc taccagatca cctcgtttcc actgctgctt tacacctgca
      901 attgtaccga ttgtcagagg gcttccggca gcgcgttcgc actgaacatg ccggtggctg
      961 ccagggattt tcatattctg cagggcgagc cgaagggatg gcgccacacg tcgccgatcg
     1021 gggtcgcggt gatatcctgg ttctgcggcg attgcggcgg ccgcctctat ggcgaccgag
     1081 ccgggcgtga ggccatcgtc aacctccgtg ccggcacgct tgacgacacg gcatggctgg
     1141 tcccggtcgc gcatatgttc acgcgaagcg cacagtcgtg ggtcgcaccc gcggccaatg
     1201 ccgaatgtca tgagaccggc cccgacgact tccggccgtt ggcccagaga tggcgggcga
     1261 tgtggcctga attcctgccg caaaaatagt tccgtcttcc cggaaccgtt ggatgccagc
     1321 caagtttagc cccggttagg ggggattatg aacttggttg aacccatcac cagcaaggcg
     1381 aaaaccggga tttgggggct ggacgacatc ctgtcgggcg gattttcgcg cgggcacgtg
     1441 tttctggtgg agggcgcgcc cgggaccgga aaaaccacca ttgcgctaca gttcctgatg
     1501 gaaggcgcgc aggccggcga gaagtgcctc tacatcaccc tgtccgaaac cgagcgcgag
     1561 ttgcgcgaag gtgcggcctc ccatggctgg tcattggagg acgggattga agttttcgaa
     1621 ttgttgccgc cggagagtct gctcgattcc gagcagcagc agagcctgct ttattcttcc
     1681 gatctcgaac tcggcgaaac gaccaaacag attttcgaag ccgtggaacg cgcacggccg
     1741 agccgggtgg tgctggacag cctttccgag atcaggctgc tggcgcaaag ttcgcttcgg
     1801 taccggcggc agatactcgc gatcaagcac tatttcgcaa agttcggcac cacggtgatg
     1861 ctgctcgacg atttgaccac cgagcttgcc gacaaaaccg tgcacagcgt ggcgcatggt
     1921 gtgctgcggc tcgaagaatt ggcgccggcc tatggcgcgg agcggcggcg cgcccgcatc
     1981 atcaaatatc gcggcgtgaa gtttcgcggc ggctatcacg atgcaaccat caccaccggc
     2041 gggctgaata tctttccgcg gctggtggcc tcggaatatc gcagcagctt tgtacgagcc
     2101 aggatgtcca gcggcatcgc cgaattggac cggcttctcg gcggcggcat cgaagcggga
     2161 tcgagcacgc tgattctcgg gcccgcgggc accgggaaat cgcttttggc gatcgtcttc
     2221 atcgccgccg cgatcgcgcg tggcgaaaag gcggcgctgt tcgtgttcga cgaggagctc
     2281 ggcctgttgt ttgcgcgcat gaaggggctc ggcgtcgacc tcgaagagat gcagcgcagc
     2341 ggcaacctct ttgtcgagca ggtcgacgcc gcggagctgt cgcccggcga attcgcgcac
     2401 cgcgtccgca agcgggtcga tgaggacgac atcaagaccg tggtgatcga cagcctcaat
     2461 ggttatcagg ctgcgatgcc cgaggaaaac tcgctgatcc tgcacatgca cgaattgctg
     2521 caatatctga accgccgggg tgctgcgaca ttcatgacgg tggcgcagca tgggctggtc
     2581 ggcgacatga aagcgccggt ggatgtaacc tacctggcgg acacggtgat gctgttgcgc
     2641 tatttcgagg cgctcggcac ggtgcggcgc gcgatgtcga tcatcaagaa gcgcaccggc
     2701 gcgcacgaat ccacgattcg cgaatatcgc atcgacggcc gcggccttac catcggtgac
     2761 ccgctcgatg gctttcaggg cgtgttgcgc ggggttccaa cttacgtcgg cgaaggccag
     2821 cccttgctgg aagagcaagt ccagtgaggc cgggcgcctc ttcggagcgt gccgtggttc
     2881 tcgcgccgac ggggcgcgac gcctccgtcg ccgcggcgct gatcagggag gcgggttact
     2941 acgccaatgt ctgcggcgat ctcgcagcaa tgttgcgcga gatcgaaggc ggcgcgggcc
     3001 ttgcggtgat cgccgatgag gcgatcaaga cggccgatct gcgcggcctg atgcgctggt
     3061 tgaacgatca gccgtcatgg tcggattttc cgatcgtgct gttgacgcat cagggcggcg
     3121 gccccgaacg caatccggac gccgtgcggc ttggacaagt gctcggcaac gtcactttca
     3181 tcgaacgtcc gttccacccc acaacgctgg taagcgtggt cggctccgcc gtcagaggcc
     3241 ggcgtcgcca gtaccagacc cgcgccattc ttcaggatct caccgaaagc gaaggcctgc
     3301 tgcagaccgc gctgagcgcc ggccgtctcg gtgcgctcga actgcatctt ccggagcttg
     3361 agttggaggc ctccgacacc tgcaaaacgt tcttcggccg caggccgggc gagccgtttt
     3421 cgtaccagga tttgctggct gcggttcatc ccgacgaccg cgcgcgacgt ctcgatgtgc
     3481 tggatcggac catcaagacc ggcagggatt acagcatcga gtaccgcaat atctggcccg
     3541 acggatcgca gcattgggtc gacgtccgcg cgcgggcggt tcgcaggccg gacggcagca
     3601 tcaaatcgct ggtcggggtt tcctccgata tcacggcgcg caaggtcgcc gaaatcgagc
     3661 gcgaaaatct gctggcgcag ctggcggccg agcgcacggc gctggcggaa ctgacggcga
     3721 cgctcgaaca acgcgtcgaa cagcgcaccg ccgatctgat gaaggaggtc gccgcgcgcg
     3781 aaaaggccca ggaacagttg cgccaggcac agaagatgga gacgatcggc cagctcaccg
     3841 gcggcgtcgc gcatgatttc aacaatctgc tgatggtggt gatgggcaac ctcgatctgt
     3901 tgcgcaagcg cattcccgac gatccgcgtc tgcaccggct gatcgacggc gcggtgctgg
     3961 gcgccgagcg cggtgcgtcg ctgacgcagc ggctgctggc gttcgcgcgg cagcaggatc
     4021 tgcgggcggt gccggtcgat ctgcgcaccc tgatccaggg catgagcgac ctgctgcaac
     4081 ggtcgctggg gccgcgcgtc gtgctgcggc tggatattcc gcaaggtctg cccgcggcgc
     4141 gcgtcgacgc caaccagctc gagctcgcaa ttctcaacct tgcgatcaac gcgcgcgacg
     4201 cgatgccgga cggcggctcg atcgacatca aggtcgtgca gtatcaggcc agcaacgatc
     4261 cggcgctgaa gtccggcggc tatctgaagc tgtcggtgat cgataccggc gtgggtatga
     4321 cggccgagat attgaagcgg gcggtcgagc cgttcttctc gtcgaagccg ctcggcaaag
     4381 gcaccggcct tgggctgtcg atggtgcacg ggcttgccgt gcaactcggc ggcacgttgc
     4441 agctctcgag cacggtggga aacggcacca ccgcgacgct gatattgccg gtggcgatca
     4501 acttgccgaa aatggaaagc ccggtgcatt cggtgcagaa ggtcaaccgc tcggcggtga
     4561 tcctgtttgt cgacgacgac ccgctgatcg cgatgtcgac caccgaaatg ctggaagatc
     4621 tcggccacca cgtgatcggt gccagttccg gcttgcacgc gctcgacatc atcaggagcg
     4681 agcagccgat cgacctgatg atgacggacc atgtgatgcc cggcatgacc ggcatcgaat
     4741 tggcggcggc gtcgcgcctg gtgcggccgt cgctgccgat cctgctcgcg accggttatg
     4801 ccgagctgcc cgagggggcc cagctcgacc tgccgcggct cgcaaagccg tatcaccagg
     4861 accagttgcg cgaccggctc gatcaattgc tgggtggaga tgagcgcgaa ccgctatccg
     4921 accatccgct cgcggcccgc ccagaagccc gcctttagca gtttcttgtc gaccttgccg
     4981 acgccggtca tcggcaattc gctaacgaat ttgatgtgct tgggcgcatg cgccgaaccc
     5041 ttgcgcgcct tgaccagatc aatcagttcc tgcgcgtcgg gccgggcacc ttcgcgcaca
     5101 acgatcaccg cggtgaccgc ctcgccccat ttgtcgtcgg gcacaccgac gacggcgacc
     5161 atcgcgacgt cggcgtgctg ggacagcacg tcctcgacct cacgcggaaa gatgttgaac
     5221 ccgccggaga cgatcatgtc cttcttgcgg tcgaggatga acatgtagcc gcgctcgtcg
     5281 gcgcgcgcga tatcaccggt atggagccag ccgttcttga gggtttccgc tgtttgttcg
     5341 ggccgcttcc agtattcggc catcacatgc ggcgcgcgca cgcaaatctc gccggcttca
     5401 ccggtttcga cctcctgatc gccatcgtcg agaattttga cgtcgcaggc ggcgatcgga
     5461 aaaccgcagg acagaaacag ttccggcgtt ttgggatcgt gatccgcctt gcgcagcacc
     5521 gagaccggat agcactcggt ctgcccgtaa agctgcgaga acaccggacc gatccgctcg
     5581 ataccctcga ccagccggct cggcgacatc gccgaggcgc catagagcag aagctcgagc
     5641 gaggagaggt cggttctgtc gagcgccgga tgatccagca gcacgtagat catggtcggc
     5701 acgaacagcg tgaagttgat gcgctcgcgc tcgatggtct tgaacaccgc gtccggatcg
     5761 aagcc
//
LOCUS       DASWGM010000404         6015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2662043,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000404 DASWGM010000000
VERSION     DASWGM010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662043"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VGA15_11825"
     CDS             <1..151
                     /locus_tag="VGA15_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX9588415.1"
                     /translation="GPSRKRALLHHFGTLKEIERASIADLGKVPGVSAESARKIFEFF
                     HAQPG"
     gene            244..861
                     /gene="pgsA"
                     /locus_tag="VGA15_11830"
     CDS             244..861
                     /gene="pgsA"
                     /locus_tag="VGA15_11830"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX9588416.1"
                     /translation="MNIATTRGSAKSSLSLPNILTYARIAAIPVVVGCVFAQSIMDGP
                     LWLRWVALAVFIAAGVTDYLDGYYARIWDQHSAFGRMLDPIADKLLVASCLLMLAAEN
                     SIHGWTLWAAIVILCREILVSGLREYLAALRVSVPVTKLAKWKTTVQLVAIGFLIAGE
                     AGEVILPPTTLIGVVLLWISAIFTIYTGWDYFRAGIHHLIKEDEG"
     gene            858..1109
                     /gene="moaD"
                     /locus_tag="VGA15_11835"
     CDS             858..1109
                     /gene="moaD"
                     /locus_tag="VGA15_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX9588417.1"
                     /translation="MKVKYFAWVRERIGKAEETIEPPASVRTVDDLIAWLARRGETYA
                     HAFETPRVIRAAIDHAHVKPDATIAGAREIAFFPPMTGG"
     gene            1140..1607
                     /locus_tag="VGA15_11840"
     CDS             1140..1607
                     /locus_tag="VGA15_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX9588418.1"
                     /translation="MTAAVTIRIQEADFDIAQEIAALTKGRTDIGAVVTFSGICRDSE
                     GDEPIAALTLEHYPEMAEAEIARHVETAMSRWPLTGLTVVHRVGRITPGENIVLVLTA
                     SKHRQAAFQAAEFLMDYLKASAPFWKREEKPAGTSWVDAHDRDDAAAARWTKS"
     gene            1607..2569
                     /gene="prmB"
                     /locus_tag="VGA15_11845"
     CDS             1607..2569
                     /gene="prmB"
                     /locus_tag="VGA15_11845"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX9588419.1"
                     /translation="MARRAKPAARRSAGSKSPKIASGELLTLLDFVRYAVSRFVEAKL
                     VFAHGTTDPVAEAAFLVCETLHLHPDQFEMFATARVTAREAKKIVDVIGRRVTTRKPA
                     AYLVNKIYMRGLPFYVDERTIVPRSFIGELLDSHFRGQRDDDDTSLIDDPLGVKSVLD
                     LCTGSGCLAVLAARAFRNAAIDAVDISRDALEVAARNVAEYGLEDRVTLHRGDLFKAI
                     GDRRYDLIISNPPYVDTEGMAELPRECRAEPKLAFDGGADGLDMIRRILQDAGQHLTP
                     EGGLLCEIGRGRERLEAAFPQLPLLWLDSEESEGEVFWIGAADL"
     gene            2722..4068
                     /locus_tag="VGA15_11850"
     CDS             2722..4068
                     /locus_tag="VGA15_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9588420.1"
                     /translation="MLDKSPRPTAVNVPNDLAAHWMPFTANRAFKKAPRLLAGAKDMH
                     YITVDGRKIIDAAAGMWCCNAGHGRTQISAAIAKQAEALDYAPPFQFGIPQAFELASR
                     IAELAPAGLDHVFFCNSGSEAADTALKIALAYHQLNGQGTRSRLIGRERGYHGVGFGG
                     TAVGGIVNNRKMFGTLLAGVDHLPDTYDREKQAFTKGEPEYGAHFADELERLVNLHGA
                     STIAAVIVEPMAGSTGVLPAPKGYLKRLREITQKHGILLIFDEVITGYGRLGFAFAAE
                     RYGVLPDMLTFAKGITNGAAPMGGVLVRDVIHDAFMSGPEHIVELTHGYTYSAHPLAC
                     AAALATLDIYRDEKLFERANKLESKFADAVMSLKGQPNVVDIRTVGLTAGIDLAPRPD
                     QPGKRGFDGLNSAFHDNDLMLRVAGDTLALTPPLIVTEDQIGEIIEKVTKVIRAVA"
     gene            4560..6005
                     /locus_tag="VGA15_11855"
     CDS             4560..6005
                     /locus_tag="VGA15_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9588421.1"
                     /translation="MIRISTIFIAICMVLVAASLGLVLYSVAGITGTESAIVALTALT
                     FLILYNAVSMRLRDRTDVGGQIADLSRGTADLARQVAEFGRRLAAIEGRVVSANSAGA
                     DRIQAVVGEISELGVLVKQLAVSVAAHDDMLASGVPAAAPAARPEDAAPNEPPPSVAN
                     EKSVAMAAVAPAKIVEEPSVSRSQAQILAAVKSAVEENRIDIYLQPMVTLPQRKVRFY
                     EAVTRLRDDKDQILTADDFIAVAEAAGLIGRIDHMVMLRCVQVLRRLMVRNKDVGIFC
                     NVAAATLGNPTTFTQCLDFLEANRALASSFVLEFKQTTFRNLGPTETEHLAALAQRGY
                     RFSIDHVSDLRIEPRELADRGVRFIKVPAALLLDQKLSSASDIHPSDLSDLLGRFGID
                     LIAERIEGERAVVDLLDFDVRFGQGFLFAPPRPLRPEGASATGGAAPNKAQVSQVSNG
                     SDNIASVNGPAIDPPRATGNAALARRAAGPA"
BASE COUNT         1107 a         1946 c         1865 g         1097 t
ORIGIN      
        1 cggcccgtca cgcaaacgtg ctttgctgca tcatttcgga acgttgaagg agatcgagcg
       61 ggcctcgatc gccgatctcg gcaaggttcc cggcgtcagc gcggaaagcg cgcggaagat
      121 tttcgagttc ttccacgccc aaccgggtta aaggtaggtt tttgattcat attattgtcg
      181 tgtccaaggg atcacgtgct ccgcacggtt gaccttcatg cttcggcggt attggtaaga
      241 cggatgaaca tcgcaaccac aagggggtcg gccaagagct cgctgtccct acccaacatc
      301 ctgacctacg cgcgcatcgc cgccatcccg gtggtggtcg gctgcgtatt tgcgcaatcc
      361 atcatggatg ggccgctgtg gctgcgctgg gtggcgctgg cggtattcat cgccgccgga
      421 gttacggatt atctggatgg ctattatgcg cgaatctggg atcagcattc ggccttcggc
      481 cggatgctgg atccgatcgc cgataagctc ctggtggcat cctgcctgct gatgctggcg
      541 gcggaaaaca gtattcatgg ctggacgctg tgggccgcca tcgtgatcct gtgccgcgaa
      601 attctggtgt cggggctgcg ggaatatctc gccgcgctgc gcgtcagcgt gcccgtgacc
      661 aaactcgcga agtggaaaac cacggttcag ttggtcgcga tcggcttttt gatcgctggc
      721 gaggctggcg aggtgatatt gccccccaca accctgatcg gcgtcgtgct gctgtggatc
      781 tccgcgattt tcacgatcta caccggctgg gattatttcc gcgccggcat tcatcacctc
      841 atcaaggagg atgagggatg aaggtcaagt atttcgcctg ggtgcgcgaa cggatcggca
      901 aggcggagga aaccatcgag ccgccggcca gcgtccgcac agtcgacgat ctgatcgcgt
      961 ggctggcgcg gcgcggcgag acttacgccc atgccttcga aacgccgcgg gtgatccgtg
     1021 cagcgatcga ccacgcccat gtcaagccgg atgcgacgat cgccggcgcc cgcgagattg
     1081 ccttcttccc gccgatgacc ggcggctaag ccgctttgac cgctacccgc gaagctcaga
     1141 tgaccgccgc tgtgaccatc cgcattcagg aagccgattt cgacatcgcg caggagatcg
     1201 ctgcgctcac caaagggcgt accgatatcg gcgccgtcgt gactttctca ggcatttgcc
     1261 gcgacagcga gggcgatgag ccgattgcgg cgttgacgct cgaacattat cctgaaatgg
     1321 cggaggccga aatcgcgcgg cacgtcgaga cagcgatgtc gcgctggccg ctcacaggcc
     1381 tgaccgtcgt tcatcgcgtc ggccgcatca cgcctggcga aaatatcgtg ctggtgctga
     1441 cggcatcaaa acaccggcag gcggcgttcc aggcggcgga gtttttgatg gactatctga
     1501 aggccagcgc gccgttctgg aagcgcgagg aaaagcctgc gggaaccagt tgggtcgacg
     1561 cgcacgatcg cgatgacgca gccgctgcgc gctggaccaa atcctgatgg cgaggcgcgc
     1621 caaaccggcc gcaagacgca gcgctggatc gaaatctccc aaaatcgcct ccggcgagtt
     1681 gctaacgctg ctcgatttcg tccgctatgc ggtgagccgt ttcgtcgaag ccaaactggt
     1741 gttcgcgcat ggcaccaccg atcccgtggc cgaggccgca ttcctggtct gcgaaaccct
     1801 gcatcttcat cccgaccagt tcgagatgtt cgcgaccgcc cgcgtcacag cgcgggaagc
     1861 caaaaagatt gtcgacgtga tcggccgccg cgtcaccacg cgcaagccgg ccgcctatct
     1921 ggtcaacaag atctacatgc gcggcctgcc gttctatgtc gacgagcgca caatcgtccc
     1981 gcgctccttc atcggcgagt tattggactc ccatttccgc ggccagcgcg acgatgatga
     2041 cacttcgctg atcgacgatc ccctgggcgt caagagcgtg ctggacctct gcaccggttc
     2101 cggctgcctt gccgtcctcg ccgcacgcgc cttccggaac gccgccatcg atgcggtaga
     2161 tatttccagg gacgcgctcg aggtcgccgc gcgcaacgtt gccgagtatg gtcttgagga
     2221 tcgggtgacg ttgcatcggg gcgacctgtt caaagccatc ggcgacaggc gttacgacct
     2281 gatcatttcc aatccgccct atgtcgatac ggaaggcatg gcggaactgc cccgcgaatg
     2341 ccgcgccgag ccgaaactgg ccttcgacgg cggcgctgac gggctcgata tgatccgccg
     2401 cattttgcaa gatgccggcc agcacctgac gccggagggc gggctgttgt gcgagatcgg
     2461 tcgaggccgc gaaaggcttg aagccgcttt tccacaattg ccgctgttgt ggctcgattc
     2521 cgaggaatcc gagggcgagg tgttctggat cggcgctgcc gatctataac cgccgcctgc
     2581 tcgtgaaacc gccccaaaaa gacggaaata tagccacccg tcggcgttcc cgtatgcggc
     2641 agaagctgat agcttgggcg aaggccgcta catcctaaaa cttaaaaagc cgccatatcc
     2701 caaaacttta aagaggcccc tatgctggac aagagcccac gacccaccgc cgtcaacgtc
     2761 cccaacgacc tcgccgcgca ttggatgccg ttcacggcca accgggcctt caagaaggcg
     2821 ccgcggctgc tcgctggcgc caaggacatg cactacatca cggtcgacgg gcgcaagatc
     2881 atcgatgccg ccgcggggat gtggtgctgc aatgccggcc acggccgcac ccagatttcg
     2941 gcggcgatcg ccaagcaggc cgaggcgctg gactatgcgc cgccgtttca attcggcatt
     3001 ccgcaggcct ttgagctcgc cagccgcatc gccgagctcg cgcccgccgg gctcgaccac
     3061 gtcttctttt gcaattccgg atcggaagcc gccgataccg cgctcaagat cgcgctggcc
     3121 tatcatcagc tcaacggcca gggcacccgc agccgcctga tcggccgcga gcgcggctat
     3181 cacggcgtcg gcttcggcgg gaccgcggtc ggcggcatcg tcaacaaccg caagatgttc
     3241 ggaacgctgc tggccggcgt cgatcatctg cccgacacct atgaccgcga aaaacaggcc
     3301 ttcaccaagg gcgagccgga atatggcgcg catttcgccg acgagctgga gcgactggtc
     3361 aacctgcacg gcgcctccac cattgccgcg gtcatcgtcg agccgatggc cggatcgacc
     3421 ggcgtgctgc cggcgccgaa gggctatctc aagcgcttgc gcgagatcac ccagaagcac
     3481 ggcattctcc tgattttcga cgaggtcatc accggttacg gccggcttgg ttttgccttt
     3541 gccgcggagc gttacggcgt gctgccggac atgctgacct ttgccaaggg catcaccaat
     3601 ggcgcggcgc cgatgggcgg cgtgctggtg cgcgacgtca tccacgacgc cttcatgagc
     3661 ggcccggagc acatcgtcga gctcacccac ggctatacct attcggcgca tcccttggcc
     3721 tgcgccgcag cccttgccac gctcgatatt taccgcgacg aaaagctgtt cgagcgcgcc
     3781 aacaagctcg agtccaaatt cgccgacgcc gtgatgtcgc tgaaaggcca gcccaacgtg
     3841 gtggacatcc gcaccgtcgg gctgacagcc ggcatcgacc tcgcgccgcg cccggatcag
     3901 cctggaaaac gcggtttcga cgggctgaac agcgcgttcc acgacaacga cctgatgctg
     3961 cgcgtcgccg gcgataccct ggcgctgacg ccgccgctga tcgtcaccga ggaccagatc
     4021 ggcgagatca tcgagaaggt caccaaggtg attcgcgcgg tggcgtagga cctctcttac
     4081 ctctccccgt cctctacggg gagaggtcga aattcgagtg caactcgaat ttcgggtgag
     4141 gggcaaggca ttgcgcttgc ctcatcatca gcattcgcgg gccggagagc ccctcacccc
     4201 aaccctctcc ccgcaagagc ggggcgaggg agtaggctgc cgccgctaaa ccttcgaaac
     4261 caatcccacg tttcgaaggt atctgtcgaa ctcccgaaac gacaacgcaa cctaaggttc
     4321 actgggtgcc gccatcgggt tgatggcgtg gatgtcggcg atcatcgtgc tgtgcggcgc
     4381 tgccatggcc gacaccatga gagccacggt tccagccgtg ccaaaagaac attagcatgg
     4441 cgaatcaaca ttaatgctaa ggtcgccgcg cttgggggaa gcgcaacaat tcgtgcgaac
     4501 agcttccgcg tttctcgcaa gcccggatga ccaagggact tgagggccgc gcgcgcaaca
     4561 tgatccgcat ttccacgatt ttcatcgcca tatgcatggt gctggtcgca gcgtcgctcg
     4621 gtttggtcct gtattccgtg gcgggcatca ctggaacgga atccgcgatc gtggcgctca
     4681 ccgcgctgac cttcctgatt ctctacaacg ccgtgtcgat gcggctgcgg gaccgcaccg
     4741 acgtcggcgg ccagatcgct gatttgtcgc gcggcactgc cgacctcgcg cgtcaggtcg
     4801 ccgaattcgg gcgccggctc gccgccatcg agggccgggt ggtgtcggcc aattcggccg
     4861 gcgccgaccg cattcaggcg gtggtcggcg agatcagcga actgggcgtg ctggtcaaac
     4921 agctcgcggt gtccgtcgca gcccatgacg acatgctggc ttcgggcgtg ccggctgccg
     4981 cgccagccgc caggccggag gatgccgcac cgaacgagcc gccgccgtcc gtcgccaacg
     5041 agaaaagcgt ggcgatggcc gcggtagcgc cggcgaaaat cgtcgaggag ccatctgtct
     5101 cgcgcagcca ggcgcaaatc ctcgccgccg tgaaaagcgc ggtggaggaa aaccggatcg
     5161 acatctacct gcagccgatg gtgaccctgc cgcagcgcaa agtgcgcttc tacgaggcgg
     5221 tgacgcggct gcgcgacgac aaggatcaga tcctcaccgc cgacgatttc atcgcggtgg
     5281 cggaagctgc cggcctgatc ggacggatcg atcacatggt gatgctgcgc tgcgtgcagg
     5341 tgctgcgccg gctgatggta cgcaacaagg acgtcgggat attctgcaac gtcgccgcgg
     5401 cgacgctcgg caatccgacg accttcacgc agtgcctcga tttcctcgaa gccaatcgcg
     5461 cgctggcgtc gtcattcgtg ctcgaattca agcagaccac gttccgcaat ctgggcccga
     5521 ccgagaccga acatctggcg gcgctggccc agcgcggtta tcgtttttcc atcgatcacg
     5581 tcagcgactt gcggatcgag ccgcgcgaac tggccgaccg cggcgtccgc ttcatcaagg
     5641 tgccggctgc cctgctgctc gaccaaaagc tgagctcggc gtccgacatt catccatccg
     5701 acctgtccga tctgctcggg cgcttcggca tcgacctgat cgccgaacgg atcgaaggcg
     5761 aacgcgccgt ggtcgatctg ctcgacttcg acgtgcgctt cgggcaagga tttttgtttg
     5821 cgccgccccg gccgttgcga cccgaggggg catctgctac cggcggggct gccccgaaca
     5881 aggcgcaggt atcccaggta tccaatggct ccgacaacat cgcttccgtc aatggtccgg
     5941 caatcgatcc gccgcgcgcg accggcaatg ccgcgctggc gcggcgcgcc gccggcccgg
     6001 cctgaccggc aatcc
//
LOCUS       DASWGM010000405         4574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_581463,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000405 DASWGM010000000
VERSION     DASWGM010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581463"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VGA15_11860"
     CDS             complement(<1..235)
                     /locus_tag="VGA15_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-carboxymuconolactone decarboxylase"
                     /protein_id="HEX9588422.1"
                     /translation="MDDQQRRDAGMAQRRKVLGNAWVDKSAANTNSFNADFIDLITRH
                     AWADIWTRPHFDERTRRVLVIGTLLALCQWDEFR"
     gene            complement(413..1033)
                     /locus_tag="VGA15_11865"
     CDS             complement(413..1033)
                     /locus_tag="VGA15_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588423.1"
                     /translation="MTRLNTSFVLGYHGCHKAIGLQAVAGKLELIQSEKAYDWLGPGV
                     YFWEGDPLRALEWAQWKKFDEPFVVGAVIDLRNCLDLMVKENLDLLASAYDSYVAQQR
                     KSGLKILENKSPRGSKSEDDKKLRFLDCAVITHLHKILDNEQSAPSSDLPAMVQPFDS
                     VRGLFEEKPEVYPGSGFFKNTHSQIAIRNLDCIVEIFVPRKFHSAA"
     gene            complement(1030..1209)
                     /locus_tag="VGA15_11870"
     CDS             complement(1030..1209)
                     /locus_tag="VGA15_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588424.1"
                     /translation="MTDAQRTAIKILIERHTAANTASPEIARAALIGEGIYTSSGSIA
                     PEYGGGEKKAADGRS"
     gene            complement(1326..2108)
                     /gene="pcaD"
                     /locus_tag="VGA15_11875"
     CDS             complement(1326..2108)
                     /gene="pcaD"
                     /locus_tag="VGA15_11875"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEX9588425.1"
                     /translation="MPMIDADGCLLNVSVEGRDGGPTLMLSNSLGCTLQMWEPQMKAL
                     TQLFRVVRYDRRGHGKSAVPPGPYSMERFGRDVLAILDDLNIKKVHWCGLSMGGMVGQ
                     WLGANAPDRFDRIILSNTACHYPDPTNWLNRIKAVKEGGIAAVADTVIAGWLTADFRE
                     REPQITANMKAMLIASPVQGYLACCEALSTLDQRELLPRIKSPTLVIAGRHDVATPVA
                     AGEFIRSQIPGASLTILDAAHISNVEQPHAFTDAVVGFLTQR"
     gene            complement(2245..3600)
                     /locus_tag="VGA15_11880"
     CDS             complement(2245..3600)
                     /locus_tag="VGA15_11880"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX9588426.1"
                     /translation="MSTSLSPLLAPMLSSAAMRAVCDDATYLQHMLGFKAALARAEAA
                     TGVIPASAADPIAKACKAGSFDLGALAETATRSGNLAIPLVKTLTANVAKADPDAARY
                     VHWGATSQDVIDTAAVLSLRAGIDALLSDVDRAIAGFAKLATKHRDTAMVARTWLQHA
                     LPMPFGLKLAEYAAALHRSRARLQRLRREGLALQFGGAAGTLAALGDKGLLVAERLAQ
                     ELELTLPAAPWHTHRDRIAEAASVFAILAGTCGKIARDLQLMMQTDVAEAFEPSGEGR
                     GGSSTMPHKRNPVAAATALAAATMAPGLAATIFAAQVQEHERSAGPWHAEWPTLPMLL
                     LVTSGALAATVDIAEGLEVDTARMRVNLEATHGLIMAEAVTMALAETIGKSEAHHLIG
                     TASKKAVADKKNLRDVLASDAEVTAHLSADKLTKLFEPMAYQGVSQALIDRLLASLDK
                     K"
     gene            3782..4228
                     /locus_tag="VGA15_11885"
     CDS             3782..4228
                     /locus_tag="VGA15_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX9588427.1"
                     /translation="MAMTMNGEVQLAAPREAVWTKLNDPEVLKVCIPGCEELEKTEDN
                     GFRAVAKMKVGPVSARFKGRVTLSDLDPPNGYKISGEGEGGVAGFAKGGATVGLADKD
                     GGTLLTYNVEAQIGGKLAQLGQRLINGSAKKLADEFFANFAKAVQG"
     gene            4390..>4574
                     /locus_tag="VGA15_11890"
     CDS             4390..>4574
                     /locus_tag="VGA15_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9588428.1"
                     /translation="MAKISLIVNGNPVNANVDPRTLLVQFLRENLRLTGTHVGCDTSQ
                     CGACVVHLDGKAVKSCTT"
BASE COUNT          870 a         1349 c         1411 g          944 t
ORIGIN      
        1 ggcgaaattc atcccattga caaagcgcaa gcagggttcc gatcaccagc acgcggcggg
       61 tgcgctcgtc gaaatgcggt cgggtccaga tgtccgccca cgcatggcgc gtgatcagat
      121 cgatgaagtc ggcgttgaag gagttggtgt tggcggcgga tttgtcgacc caggcatttc
      181 cgagcacctt gcggcgctgc gccatgccgg cgtcgcggcg ttgttgatcg tccattgttc
      241 ctctctccag gttcgaccga cgtatggtgt tcaatgatga cgctcatgcc gcgctctcga
      301 ccaatgggaa aaaagtgaga ttaaattcgc ggcggttggc cgatcggggg cttcgttgcg
      361 ctcggtggat tatgaagcgc gccctccagt attgcgcgaa acataccaaa ggctaggcag
      421 ccgaatgaaa ttttcgcggc acaaaaatct cgacgatgca gtcgaggttt ctgatggcga
      481 tctgtgaatg cgtgttcttg aagaatccgc ttccgggata aacttctggt ttttcctcga
      541 acagacctcg gacggaatca aacggctgga ccatcgcggg aagatcactg cttggagccg
      601 attgctcatt gtccaggatt ttatgtagat gtgtaattac ggcacaatcc aaaaaacgaa
      661 gctttttgtc gtcctctgac tttgagccgc gggggctttt attctcaagt attttcaagc
      721 cggattttct ttgttgtgcg acatagctgt cataagccga tgccagcaaa tccagatttt
      781 cctttaccat cagatcgaga caatttctaa ggtcgataac cgcgccaaca acgaatggtt
      841 cgtcgaattt tttccactgt gcccattcca aggcgcgtaa cggatcaccc tcccagaagt
      901 agactcctgg accaagccaa tcgtaggctt tttcgctctg aattaactct aatttgccgg
      961 ctactgcctg taggccaatg gctttgtggc agccgtgata tcctaatacg aacgatgtgt
     1021 ttagccgcgt cacgaccgtc cgtcagcggc cttcttctcg ccaccaccgt actcaggcgc
     1081 aatgcttcct gaactggtgt agatgccctc tccaataaga gctgcccggg cgatttccgg
     1141 cgatgcagta ttagctgcgg tgtgccgttc aatcaaaatc ttgatcgctg tgcgctgggc
     1201 atcagtcatt ttgggtagtc ctttcaaaaa catatagcat tgagtcctaa tttttccaat
     1261 atacggcatc atattaccgg cggcttatta ccggctacct cccggcaagc attctttgtc
     1321 gcgcgttagc gttgcgtcag gaaaccgacc acggcgtccg taaaagcgtg cggttgttcg
     1381 acattggaaa tatgcgcggc gtcgagaatg gtgaggctgg cgccgggaat ctggctgcgg
     1441 atgaactcgc cggccgcgac cggcgtcgcc acgtcgtggc ggccggcgat caccagcgtc
     1501 gggcttttga tcctgggcaa aagctcgcgc tggtcgagcg tgctcagggc ttcgcagcag
     1561 gcgagatagc cctgtaccgg cgaagcgatc agcatcgcct tcatattcgc tgtgatctgc
     1621 ggttcgcgct cgcggaaatc cgccgtcagc cagcccgcga tgacggtgtc ggccaccgcg
     1681 gcgatgccgc cttccttcac cgccttgatg cgattgagcc agttggtcgg atcgggatag
     1741 tggcaagctg tgttggagag aatgatccgg tcgaagcgat cgggcgcgtt ggcgccgagc
     1801 cactgtccga ccatgccgcc catcgacaaa ccgcaccaat gtaccttctt gatgttgagg
     1861 tcatcgagga ttgccagcac atcgcggccg aagcgttcca tggaataggg gccgggcggg
     1921 acggccgatt tgccatggcc gcgccggtca tagcggacca cgcgaaacag ctgggtcagc
     1981 gccttcatct gcggctccca catctgcaaa gtgcagccca gcgaattcga tagcatcagg
     2041 gtcggcccgc cgtcgcggcc ttcgacggat acgttgagca ggcaaccgtc ggcatcgatc
     2101 atcggcattg tcgggttcct cgtcatgcgc ggactaaaag cgcgaagcgt gtcttcgcac
     2161 tggatgatcc gcgcatccat ctagaaaaga acattctatc caagaagatg gattgccggg
     2221 tcaagcccgg caacgacatc tggactactt tttgtcgagc gaagcgagca gccggtcgat
     2281 cagagcttgc gagacgccct gataggccat cggctcgaac agttttgtca gtttgtccgc
     2341 gctcaaatgc gcggttacct ctgcgtcgct tgccagtacg tcgcgcagat ttttcttgtc
     2401 ggcgacggcc ttcttgctcg cggtcccgat gagatgatgg gcctcgcttt tgccgatcgt
     2461 ctcggccagc gccatcgtca ccgcttcggc catgatcagc ccgtgggtgg cctcgagatt
     2521 gacgcgcatg cgcgcagtat cgacttccag cccttcggcg atatcgacgg tcgcggcgag
     2581 cgcgcctgac gtgaccagca gcagcattgg caaggtcggc cattccgcgt gccacggccc
     2641 ggcgctgcgt tcgtgttcct gcacttgcgc cgcgaatatc gtcgccgcca gaccaggcgc
     2701 catggtggct gctgcgagcg cggtcgcggc agcgaccggg ttgcgcttgt gcggcatggt
     2761 cgaagaaccg ccgcggcctt cgcccgaggg ctcgaacgcc tcggcgacat cggtctgcat
     2821 catcagttgt aggtcgcggg cgatcttgcc gcaggtgccc gctagaatcg cgaacacgga
     2881 agcggcttct gcgatgcggt cgcggtgggt gtgccacggc gctgccggca gcgtcagttc
     2941 aagttcctgt gccagtctct ctgcgaccag caatccctta tcgccgagcg ctgccaacgt
     3001 gccggcggcg ccgccgaact gcagggccag tccctctctg cgcaatcgtt gcaatcgtgc
     3061 gcgcgagcga tgcaatgcgg cggcatattc ggcaagcttg agcccgaacg gcatcggcaa
     3121 ggcatgctgc agccaggtgc gggccaccat cgccgtgtcg cgatgcttcg ttgcgagctt
     3181 ggcaaagccc gcgatggcgc gatcgacgtc ggaaagcagg gcgtcgatgc cggcgcgcaa
     3241 gctcagcacg gcagcggtat cgatcacgtc ctggctggtg gcgccccagt gcacgtagcg
     3301 cgcggcgtcc ggatctgcct tggcgacgtt agcggtgagc gtcttgacca gaggtattgc
     3361 gagattgccg gagcgcgtgg ccgtctcggc cagggcgccg agatcgaacg acccggcctt
     3421 gcaggctttg gcgatcgggt ccgccgcgct cgcggggatc acgccggttg ccgcctcggc
     3481 gcgcgccagc gcggccttga aaccgagcat gtgctggaga tacgtcgcgt cgtcgcagac
     3541 ggcgcgcatg gcggcgctcg acagcatggg cgcgagcaaa ggcgaaagcg aagtgctcat
     3601 gatacgcgcg acctaaccat ccctgccggc cgctgccaat cccaaagcgc aaatcccaag
     3661 gcgcccgcga gcctgttgcg ctgcgaatat gcatcgctca tggtcgcatc atccccttcc
     3721 ttttggggcc cgcgtgcttt atttgggggc cagaccacgc gcaatccggg aggcacccac
     3781 gatggccatg acaatgaacg gcgaggtcca gcttgcggcg ccgcgcgagg cggtgtggac
     3841 caaattgaac gatcccgaag tgctcaaggt ctgcatcccc ggctgcgagg aactggagaa
     3901 gaccgaagac aacggttttc gcgcggtggc caagatgaag gttgggccgg tgtcggcccg
     3961 gttcaagggg cgcgtgacgc tgagcgatct cgatccgccc aacggctaca agatatcggg
     4021 cgaaggtgag ggcggtgtcg ccggcttcgc caagggcggc gcgaccgtgg ggttggccga
     4081 caaggacggc ggtacgctgt tgacctacaa tgtcgaggcg cagatcggtg gcaagctcgc
     4141 ccagctcggg cagcggctga tcaacggttc agccaagaaa ctggccgacg aattttttgc
     4201 gaatttcgcc aaggcggtgc aaggctgatt ccctgaggag gctagttcct ccgaggaccg
     4261 ccgaaccata ccaggccatg ccccaaggtg aggttgctca ttgccgggat ggcccatatt
     4321 atggcgctgg aatgatttta atgactgtgc tgccgtgcaa tgcagcagcg cagatgaaga
     4381 gagtgctgaa tggccaagat ttccctgatc gtgaacggca atcccgtcaa cgccaatgtc
     4441 gacccccgca ccttgctggt gcagttcctg cgcgagaatc tgcggctgac cggcacccat
     4501 gtcggctgcg acacctcgca atgcggcgcc tgcgtcgtgc atctcgacgg caaggcggtc
     4561 aaatcctgca ccac
//
LOCUS       DASWGM010000406         3773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_165124,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000406 DASWGM010000000
VERSION     DASWGM010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165124"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VGA15_11895"
     CDS             complement(<1..701)
                     /locus_tag="VGA15_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9588429.1"
                     /translation="MRDRLIFASQLLFTLLVAAFLVVPAILSISAGVTVNYFRGISSG
                     VTLQWVFQVWDLYAGTILLSFVIAFAALAVTLVVGVPSAYALHVRGGRVSRIVEEIIT
                     LPLAIPGLAIALALLLTYGSFGSFRRSWLFILTGHVIFTMPFMVRSVMAVFATVDIKT
                     LDEGAASLGASPWRRFCDVIVPNAVPGILAGSLMVVTLSLGEFNLTWMLHTPLTKTLP
                     IGLADSYASMRLEVAS"
     gene            complement(706..1521)
                     /locus_tag="VGA15_11900"
     CDS             complement(706..1521)
                     /locus_tag="VGA15_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588430.1"
                     /translation="MPHKSFVWLCLLPLAVVTTAFFLLPMARLVVTGAEGPQGLAGYL
                     AILTEPRYRATLINTVVLATATTIVTLAIATVAGMFLQRHRFPGRAVLIAMLTFPLAF
                     PGVVVGFLIILLAGRQGLIGDLSNRLFGEKLVFAYSIYGLFLGYLYFSIPRVILTIMA
                     AVQKLDVGLEEAARSLGASPWAVQRDVVLPALGPAFVASGAIAFATAMGAFGTAFTLA
                     TNIDVLPMLIYTEFTLAANFSTSAALSVGLGIIAWAILALARSFSGSTVAAAG"
     gene            complement(1561..2568)
                     /locus_tag="VGA15_11905"
     CDS             complement(1561..2568)
                     /locus_tag="VGA15_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588431.1"
                     /translation="MKTVRLVLALLALSLLAPLQSAKAADVICYNCPPEWADWASMLK
                     AIKTDLSYDIPHDNKNSGQALAQILAEKSNPVGDIGYFGVTFGMKAKAEGALEPYKPA
                     NWDQVAPGLKDPDGYWTTIHSGTLGLFVNKDALGGKPVPACWKDLLKPDYRGMVGYLD
                     PSSAAVGYVGAVAINLSLGGSAANFEPAITFFKELRKNDPIVPKQTSYARVGSGEMPI
                     LLDYDFNAYRAKYSEKGNFEFVIPCEGSVVFPYVVGLVKNAPDKEKAKKVIDYLLSDK
                     GQTIWTNAYLRPARQIELPEAVKAKFLPDSDYARAKSVDWGQMETVQKGFVDRYLAEV
                     R"
     gene            complement(2734..>3773)
                     /locus_tag="VGA15_11910"
     CDS             complement(2734..>3773)
                     /locus_tag="VGA15_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BtaA family protein"
                     /protein_id="HEX9588432.1"
                     /translation="IVTISSGGCNALSYLTARPAQVYAVDLNEAHLSLLELKLAGLRA
                     LSDYADFWQFFGEGASAANAGLYRDRLRPVLGADARAYWDKRNSTGRPRYAYFTDGFF
                     RHGVLGRFIGMAHVLAKFARIDLAALLHEKIGSPARVQALDRLHRLFHSPLARLLTRT
                     PALLFSLGIPPRQRALLGGGAPLNEVLHDRLLRLIDVHPNETNYFAWQALSRKYAGPD
                     DRCLPAYLQRSQFERMRDGAGLIIPVHANLRLFLESLPAREVDAVVLLDSQDWMAPEE
                     IRALWNAIDRTGSNNVRVIFRTAGAASPLDSAELAPLQQIWCRDEKRSAIGFARDRSG
                     IYGGFHCYGRR"
BASE COUNT          832 a         1142 c         1174 g          625 t
ORIGIN      
        1 gaggctactt ccagccgcat cgaggcgtag ctatcggcaa ggccgatcgg aagcgttttg
       61 gtcagtggcg tatgcagcat ccaggtcagg ttgaattcgc cgagcgaaag cgtaaccacc
      121 atcaggctgc ctgcaagaat ccccggcacc gcattgggaa cgatcacgtc gcagaagcgt
      181 cgccatggcg atgcgccgag cgacgccgcg ccttcgtcga gcgtcttgat gtcgacggtg
      241 gcaaataccg ccatgaccga tcgcaccatg aacggcatgg tgaagatgac atggccggtc
      301 aggatgaaaa gccacgagcg acggaagctg ccgaagctgc cataggtgag cagcagcgcc
      361 agggcgatcg caagcccggg aatcgccagc ggcaaggtga tgatttcctc gacgatccgc
      421 gagaccctgc cgccgcggac atgcaaggca taagccgagg gaacgccgac gaccagggtg
      481 acggcgagag ccgcgaatgc gatcacgaac gacagcagga tggtgccggc atagagatcc
      541 cagacctgaa atacccattg cagcgtcacg cccgaggata tcccccgaaa gtagttcacg
      601 gtgacgccgg ccgaaatcga caggatggcg ggcacgacca gaaacgcggc gaccagcagc
      661 gtaaacagca gctgactggc aaagatcagg cgatcgcgca tcgtctcatc cggccgccgc
      721 gaccgtgctt ccgctgaacg agcgggccag cgccaggatc gcccaggcga tgatgccgag
      781 accgaccgac aacgccgccg atgtcgaaaa attcgccgca agcgtgaact cggtatagat
      841 cagcatcgga agcacgtcga tattcgtcgc cagcgtgaac gccgtgccga acgcgcccat
      901 cgcggtggca aaggcgatcg cgccagaggc cacgaaagcc ggtcccagcg ccggcagcac
      961 gacatcgcgc tgcacggccc acgggctcgc gccaagcgaa cgcgcggcct cttccaggcc
     1021 gacgtcgagt ttctggaccg cggccatgat ggtgagaatg acacgcggga tcgagaaata
     1081 gagatagccg aggaagagcc cgtagatcga ataggcaaac accagtttct cgccgaacag
     1141 gcggtttgaa agatcgccga tcaagccctg tcggcccgcg agcagaataa tcaggaagcc
     1201 gacgaccacg ccgggaaacg ccagcggaaa ggtcagcatc gcgatcagca cggcccggcc
     1261 cgggaagcga tggcgttgca ggaacattcc cgcgaccgtc gcgatggcca gcgtaacgat
     1321 cgtggttgcg gtcgccagca cgacggtgtt gatcaaggtg gcgcggtagc gtggctcagt
     1381 caggatcgcg agatatccgg ccagcccttg cggaccttca gctccggtca ccaccagccg
     1441 cgccatcggc aacagaaaga atgccgtcgt caccacggcg agcggcagca gacacagcca
     1501 aacgaaggat ttgtgcggca tgaaacaatg gtgcccttaa gagctaggca ccatactgca
     1561 tcagcggacc tcggcgagat agcggtcgac aaaacctttt tgcacggttt ccatctggcc
     1621 ccagtcgacg ctttttgccc gggcgtagtc gctgtccggc aagaacttcg ccttgaccgc
     1681 ctcaggcaat tcaatttgac gcgccgggcg caagtaggcg ttggtccaga tcgtctgccc
     1741 cttgtcggac aacagatagt cgatcacctt cttggccttt tccttgtccg gcgcattctt
     1801 gacgaggccg acgacgtagg gaaataccac cgacccctcg cacgggatca caaattcaaa
     1861 attgcccttc tcggaatatt tcgcgcgata ggcgttgaaa tcataatcca gcaggatcgg
     1921 catctcgccg gaacccacgc gggcatagga cgtctgtttg gggacgatcg gatcgttctt
     1981 gcgcagttcc ttgaagaagg tgatcgccgg ctcgaaattg gccgcggagc cgccgagcga
     2041 cagattgatc gcaactgccc cgacatatcc gacggcggcc gatgagggat cgaggtaacc
     2101 gaccatgccg cgataatccg gcttcagcag atccttccag caagccggca ccggtttacc
     2161 accgagcgca tccttgttca cgaacaggcc gagcgtgccg gaatgtatgg tggtccaata
     2221 accgtccggg tccttcaatc caggggcaac ctgatcccag ttggcgggct tgtacggctc
     2281 cagcgcacct tccgccttcg ccttcatgcc gaaggtgact ccgaaatagc cgatatcgcc
     2341 gacaggattg cttttctccg cgaggatctg cgccagcgcc tggccggagt tcttgttgtc
     2401 gtggggaata tcgtagctga gatcggtctt gatggccttg agcatcgagg cccagtccgc
     2461 ccattccggc gggcagttat agcagatgac gtcggccgct ttggcagatt gcagcggcgc
     2521 caggagcgac agcgcaagca aggccaggac aagacggacg gtcttcatga aggtcgctcc
     2581 gagtaaaaag ggagtgcatc cgcaagatcg ggatttcaac accaacgagg tgaccaagta
     2641 taggtcgcgt atgaaggatt tgcgacggtc ggtaattccg ctgtgcaatc gacctgggcg
     2701 ctacctacgg tattacccat tcgcgcttca ccgtcagcgc cgtccatagc agtgaaatcc
     2761 gccgtagatt cccgatctgt cccgcgcgaa tccgatcgcg cttctttttt cgtcgcggca
     2821 ccagatctgc tggagcggcg caagctcggc gctatccagc ggagacgccg cgcccgcggt
     2881 ccgaaagatc acgcggacgt tgttgctgcc ggtgcggtcg atcgcattcc agagcgcccg
     2941 gatttcctcc ggcgccatcc agtcctgcga atccagcagc acgacggcat cgacctcgcg
     3001 tgccggaagg ctctccagga acagccgaag attggcgtgc accggaatga tcaagccggc
     3061 gccgtcgcgc atgcgttcga actggctgcg ctgcaggtaa gccgggaggc agcgatcgtc
     3121 aggacctgcg tatttgcgac tgagcgcctg ccaggcgaaa taattggtct cgttgggatg
     3181 cacatcgatc aggcgcagca gccggtcatg aagcacttcg ttgagcggag cgcctcctcc
     3241 cagcaaggcc cgctgccggg gcgggatgcc aaggctgaac agcaaggccg gagtccgggt
     3301 tagcaggcgg gccagcggcg aatgaaacag gcgatgcaac cggtccaggg cctgaacgcg
     3361 cgcgggcgag ccgatttttt catgcaacaa tgccgcgagg tcgatccgtg cgaatttggc
     3421 cagtacatgg gccatgccga tgaaccggcc gagcacgccg tggcgaaaga agccgtcggt
     3481 gaaataagca tagcgcggac ggccggtcga attccgcttg tcccaatatg cgcgcgcgtc
     3541 cgcgccgagc accggtctta gccgatcgcg ataaagcccg gcgttggcgg ccgaggcgcc
     3601 ttcgccaaag aattgccaga agtcagcgta atcggacaaa gcccgaaggc cggcgagctt
     3661 gagctcgagc aacgaaagat gagcctcatt gagatcgact gcgtacacct gggccggccg
     3721 cgccgtcaga tacgacagcg cattgcatcc gccggatgaa atggtgacga tgg
//
LOCUS       DASWGM010000407         4916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_722802,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000407 DASWGM010000000
VERSION     DASWGM010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722802"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VGA15_11915"
     CDS             <1..1248
                     /locus_tag="VGA15_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9588433.1"
                     /translation="QFDFFLFGFYATAISKAFFPPGNETTALLLTFTTFWLGALMRPV
                     GAIVLGAYIDRIGRRLGLIVTLAIMAMGTVFIAICPTYASIGIAAPIFVLVGRLLQGF
                     SAGVELGGVSVYLFEISTPGNKGFYTSFQSASQQVAIFAAAIIGFALNEAVQGDTLVA
                     FWTGADGIGKWRIPFFVGCLIIPFIFVLRRTLEETPAFLAMKKHPSTSEVFSSAAANW
                     KIILLGTLMVAMTTVTFYFVTVYTPTFGKNVLKLTTRDALLVTLCVAVTNFIWLPIGG
                     AISDRIGRKPVLLAIAGLSLITAYPMLSWLVVEPTFTKMLIVELVFSFYFGVYNGAMV
                     AALTEVVPAHVRTTCFSLAFSLAAALFGTFTPLAATWLIDRTGDKASPGFWLMFAAAS
                     GIIATLAIFRGGETIEARQAVAA"
     gene            1320..2717
                     /gene="tcuA"
                     /locus_tag="VGA15_11920"
     CDS             1320..2717
                     /gene="tcuA"
                     /locus_tag="VGA15_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX9588434.1"
                     /translation="MVDFNRKFDVLVIGGGNAALCAAVSARRAGASVLVLEGAPKFYR
                     GGNTRHTRNMRCAHDAATDILTGPYTEQEFWEDLLRVTGGETDEELARHMIRESKDIL
                     GWVVEQGVRWQPSLGGTLSLGRTNSFFLGGGRAMLNALYLTAEQLGVEILYDAEVLDL
                     QIDHGMFLSADLKQGDGRVTVRASTLVAAAGGFEANIEWLKEYWGDAADNFLIRGTPY
                     NRGSILKMLLNKGVQEIGDPTQCHAVAIDARAPKFDGGIITRHDSVVFGIVVNKNAER
                     FYDEGEDIWPKRYAIWGRLVAAQPDQIAYIIFDASSRNSFMPTLFPPIEASSIAELAG
                     KFELDPAALEKTVGDFNAAVRPGTFDHTILDDCRTEGLAPPKTHWARKIETPPYYAYP
                     VRPGITFTYLGTRVNKQARMLMKDGKPAANMFAAGEIMAGNVLGKGYAAGMGMTIGSV
                     FGRIAGREAAQNARN"
     gene            2752..3795
                     /locus_tag="VGA15_11925"
     CDS             2752..3795
                     /locus_tag="VGA15_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEX9588435.1"
                     /translation="MSRIPEVILAAALTVAASGAAYAAEPIALRDMGSFHIGGRLVEI
                     SGKPVKEITFTPGGVPARVDPNGTYQVEQMYVQYFLPANETGAYPLLLWHGGGLTGVT
                     YETTPDGREGWLNYFLRKGWSVYNSDAVERGRAGWAQYPDIFKSEPVFLTTSNPFERF
                     RIGDGPGSYDPNPTKRKLMPGSQFPNEGYENFVKQNVPRWTTNDDATLAAYIAEIDRV
                     CPCIILFHSQAGTFGFKAAQARPDKVKALIAVEPAGVGDPAKIDTLKSIPSLLIYGDF
                     IERDSRWPKIRATGIAFADGIKAGGGSADVVDLPKAGISGNSHMMMMDKNNTEVAALI
                     QTWLEGKGLTKKP"
     gene            3825..>4916
                     /gene="tcuB"
                     /locus_tag="VGA15_11930"
     CDS             3825..>4916
                     /gene="tcuB"
                     /locus_tag="VGA15_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HEX9588436.1"
                     /translation="MHGTKILEEANRLMTVCNSCRYCEGLCAVFPAMEMRRSFSDGDL
                     NYFANLCHACGACYTDCQFSPPHEFNVNVPKTLAVARADSYAAYAWPRAFSGVFARNG
                     LAISIIAALSVAAFIFGFAAFNDRQVLFGIHTGPGAFYALMPHNAMAALFGAAFLYAI
                     AALAMGVRAFWRDIGEPIGMLVDPPSLWQAMMDAGQLRYLDGGGVGCVNEDERPTDRR
                     KIFHHFTFYGFLLCFAATCVATLYHYLIAREAPYPWWDLPVVLGTLGGIGLLIGPAGL
                     LAEKWKRDPALVDEKRTGMDVAFIAMLFLASLTGMALLLLRDTAAMGPLLALHLGLVF
                     SLFITMPYGKFVHGIYRYVALVRYARERRM"
BASE COUNT          886 a         1549 c         1487 g          994 t
ORIGIN      
        1 caattcgatt tcttcctgtt cggcttctat gccacggcga tttccaaggc gttctttccg
       61 cccggcaatg aaaccaccgc gctgttgctg acgttcacga cattctggct tggcgcgctg
      121 atgcgcccgg tcggcgccat cgtgcttggc gcctatatcg accgtatcgg ccgccgtctc
      181 ggtttgatcg tgacgctggc gatcatggcc atgggcacgg tcttcatcgc gatctgtccg
      241 acctatgcga gcatcggcat tgcggcgccg attttcgttt tggtcggaag actgctgcag
      301 gggttctccg ccggcgtcga gctcggtggc gtctcggtct atctgttcga gatttcgacg
      361 cccggcaaca aaggcttcta cacctcgttc caatccgcca gtcagcaggt ggcgattttc
      421 gcagcggcga tcatcggttt cgcgctgaac gaggccgtgc agggcgatac ccttgtcgca
      481 ttctggactg gggcagacgg aatcggcaaa tggcggatcc cgttcttcgt cggctgcctc
      541 atcattccct tcatcttcgt gcttcgccga acgctggaag aaacgccggc gttcctggcc
      601 atgaagaaac atccgagcac atcggaggtt ttcagttcag cggcggcaaa ttggaagatc
      661 attctccttg gaacgttgat ggtcgcgatg accaccgtga cgttctattt tgtcacggtc
      721 tacacaccga cgttcggcaa gaacgtgctc aagctgacga cgcgcgacgc gctgctggtg
      781 accttgtgcg tcgccgtcac caactttatc tggctgccga tcggcggcgc catctccgac
      841 cgtatcggcc gcaaaccggt gctgctggcg attgctgggc tgtcgctgat cacggcctat
      901 ccgatgctct cgtggctcgt cgtcgaaccc accttcacca agatgctcat cgtcgagctg
      961 gtgttctcct tctacttcgg tgtctacaac ggcgcgatgg ttgccgcact gaccgaagtg
     1021 gttccagccc atgtgcgaac cacctgcttc tcgctggcgt tcagtctcgc cgcggccttg
     1081 ttcggcactt tcaccccgct ggccgcgacc tggctgatcg acagaaccgg cgacaaggct
     1141 tcgcccggat tctggctgat gttcgccgcg gccagcggta ttatcgccac cctggccatc
     1201 ttccgcggcg gtgaaaccat tgaagcacgc caagccgtcg ccgcctgaaa ccaggcgctg
     1261 gccaggcgct cgcctggccg ccggtttgat ttccatgcaa acaaagataa caagacggca
     1321 tggtcgattt taatcgtaaa ttcgatgtgc tggtgatcgg cggcggcaat gccgcgctgt
     1381 gcgccgcggt cagcgcgcgg cgtgccggcg cctcggtgct cgtgctcgaa ggcgcgccaa
     1441 aattttaccg gggcggcaat acccggcata cgcgcaatat gcgctgcgcc cacgacgccg
     1501 caaccgacat tctcaccggg ccctataccg agcaggaatt ctgggaagac ctcttgcgcg
     1561 tgaccggcgg cgagaccgac gaggagctgg cgcggcacat gatcagggag tccaaggaca
     1621 tcctgggctg ggtcgtcgaa cagggcgtgc gctggcaacc ctcgctgggt ggcacgctga
     1681 gcctcggccg aaccaattcg ttcttcctcg gcggcggccg ggcgatgctc aatgcgcttt
     1741 atctgacggc cgagcagctc ggtgttgaaa tcctctacga cgccgaggtg ctcgatctcc
     1801 agatcgacca cggcatgttc ctctccgctg acctgaagca gggcgacggc cgtgtgaccg
     1861 tgcgcgcctc gaccctggtc gccgccgccg gcggattcga ggccaacatc gaatggttga
     1921 aggagtattg gggcgacgcc gccgataatt tcctgatccg cggcacgccc tataatcgcg
     1981 gctcgatcct caagatgctg ctgaacaagg gcgttcagga gatcggcgat cccacgcaat
     2041 gccacgcggt ggcgatcgac gcccgcgcgc cgaaattcga cggcggcatc atcacccggc
     2101 acgattccgt ggtgtttggc atcgtcgtca acaagaacgc cgaacggttt tacgacgagg
     2161 gcgaagacat ctggccgaag cgctacgcga tctggggccg cctcgtcgca gcccagccag
     2221 accagatcgc ctacatcatc ttcgacgcga gttcgcgcaa cagtttcatg ccgacgctgt
     2281 tcccgccgat cgaggccagc tcgatcgccg agctggccgg caaattcgag ctcgatccgg
     2341 cggcgctgga aaagaccgtc ggcgacttca atgcggcggt gcgccccggc accttcgatc
     2401 acaccatcct tgacgattgc cgcaccgagg gcctcgcgcc gccgaagacc cactgggcgc
     2461 ggaaaatcga aacgccgcct tattatgcct acccggtccg gcccggcatc accttcacct
     2521 atctcggcac ccgcgtgaac aagcaggcgc gcatgctgat gaaggacggc aaacctgcgg
     2581 ccaacatgtt cgccgccggc gagatcatgg ccggcaatgt gctgggcaaa ggctacgcgg
     2641 ccggaatggg catgaccatc ggcagcgtgt tcggacggat cgctggacgg gaagcggcgc
     2701 aaaatgcaag gaactagacg agcaactggg caagcaactc gaggagggat catgtcgcgc
     2761 attcccgaag tcatccttgc cgcggctttg acggttgccg catcaggcgc cgcatatgcc
     2821 gctgagccga tcgccctgcg cgacatgggt tcattccata tcggcggccg gctggtcgaa
     2881 atatcaggca agccggtcaa ggaaatcacc ttcacgcccg gcggcgtgcc ggcgagggtc
     2941 gatcccaacg gcacctatca agtcgagcag atgtacgtgc agtattttct gcccgccaac
     3001 gagaccggcg cctacccgct gttgctgtgg catggcggcg gtctgacagg tgtcacctac
     3061 gagaccaccc cggacggccg cgaaggctgg ctgaattact ttttgcgcaa gggctggagc
     3121 gtctacaatt ccgacgccgt cgagcgtggc cgcgccggct gggcgcagta tcccgacata
     3181 ttcaagagcg aaccggtatt tttgaccacc agcaatccgt tcgagcggtt tcgcattggc
     3241 gacggtccgg gctcctacga tccgaacccg acgaagcgga agctgatgcc cggcagccag
     3301 ttcccgaacg aaggctacga aaacttcgtc aagcagaacg tgccgcgctg gaccaccaat
     3361 gatgatgcga cgctggccgc ctacatcgcc gagatcgatc gggtctgtcc gtgcatcatt
     3421 ctgtttcaca gccaggccgg cactttcggc ttcaaggcgg cgcaagcgcg gcccgacaag
     3481 gtcaaggccc tgatcgcggt cgagcctgcc ggcgtcggcg atccggcgaa aattgatacg
     3541 ttgaaaagca tcccgtcgct gctgatctat ggtgacttta tcgaacgcga ttcgcgctgg
     3601 ccgaaaattc gcgccaccgg cattgccttt gccgatggca tcaaggctgg cggcggcagc
     3661 gccgatgtcg tcgatctgcc caaggccggc atatccggca attcgcacat gatgatgatg
     3721 gacaagaaca acaccgaggt tgccgcgctg atccagactt ggctggaagg caaagggctg
     3781 accaaaaagc cctgagccat ttttcgaaag cgaaactcca caacatgcac ggaaccaaga
     3841 tcctggagga agccaaccgc ctgatgacgg tctgcaattc gtgccgctat tgcgaaggcc
     3901 tttgcgcggt atttccagcg atggaaatgc gccgctcgtt ctccgacggc gacctgaatt
     3961 atttcgccaa tctctgccac gcgtgcggcg cctgctacac cgactgccag ttctcgccgc
     4021 cgcacgaatt caacgtcaat gtgccgaaga cgcttgccgt cgcccgcgcc gactcctacg
     4081 ccgcctatgc ctggccgcgc gccttttcgg gcgtattcgc gcgcaacggc cttgccatca
     4141 gcatcatcgc ggccttgagc gtcgcggcat ttattttcgg ttttgccgct ttcaacgatc
     4201 ggcaagttct atttggaatc cataccgggc ccggcgcctt ctacgcgctg atgccgcata
     4261 acgcgatggc ggctttgttc ggcgccgcgt tcctctatgc gatcgcggca ctggcgatgg
     4321 gtgtgcgcgc cttctggcgc gatatcggcg aaccgatcgg catgctggtg gatccgcctt
     4381 cgctctggca ggcgatgatg gatgccggac agctgcgcta tctcgatggc ggcggcgtcg
     4441 gctgcgtcaa cgaggacgaa cggccgaccg accgacgcaa gatttttcac cattttacgt
     4501 tctacggttt tttgctgtgc ttcgccgcga catgcgtggc gacgctgtat cattacctga
     4561 tcgcgcgcga ggcgccctat ccgtggtggg acctgccggt ggtcttgggc acgctcggcg
     4621 gcattgggct tctgatcgga cccgccggac tgctggccga aaaatggaag cgcgacccgg
     4681 cgctggtcga cgagaagcgc accggcatgg atgtggcgtt catcgccatg ctgttcctcg
     4741 ccagtttgac cggcatggcg ctgttgcttt tgcgcgatac ggctgcgatg gggccgctgc
     4801 tggcgctgca tctaggctta gtgttttccc tgttcatcac catgccctac ggcaagttcg
     4861 tgcacggcat ctatcgctat gtcgcgctgg tgcgctatgc gcgggagcgg cggatg
//
LOCUS       DASWGM010000408         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_581635,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000408 DASWGM010000000
VERSION     DASWGM010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581635"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            519..1715
                     /locus_tag="VGA15_11935"
     CDS             519..1715
                     /locus_tag="VGA15_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588437.1"
                     /translation="MAISRRDVLLGSAGALGAATFSFPKPSIAQSEPIKIGCLAAMTG
                     PSSAPTIGFNRGVNFAVEAINAAGGVKGRKIEIVMRDTQGDPTKAVNATQELISQAKV
                     HAIWGPLNSGEALATTPIMARARIPDIHPCVVESLIDPAKFPNAFRMAPSNSQWDDAV
                     RNYCLNILKAKKVAVIGDTTGYGVTALGASVAAFKKDGAEVVYQANIDSTQPDMTPDM
                     LRAKNAGAEVIVVWSVSTGMEARMFNTRAAMNWDVSFVGHPSMSSGEIAGLVEKPANW
                     KKVYAIGYKSCSYDAAGKLPPKSQDLVDRLLKANVVISDTLLWWIAGGIDAIELIAKG
                     VAESGSTDSEGIIKYWNTLSKYPGYFGDYTFTPTQHNGYPTDEIVMSEASTAKNGTFA
                     VAPGYT"
     gene            1738..2595
                     /locus_tag="VGA15_11940"
     CDS             1738..2595
                     /locus_tag="VGA15_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588438.1"
                     /translation="MLASILASGLAAGAIYALVGVTYNTMFSTSRVMSFTAGQLAMLG
                     GVFGSMFTLKLGMPVVVGLLLTLLGCAIIGIITEFVAVRPVLKSLDQHLYVLSTLALA
                     LMIQQVAAIKWGTEPQPFPRLIGWGSGVFDEKFWLPVVACAITIIGLEYLYRRTLVGR
                     AFLAIAEDNFAARALGLPERNLRVASYALAGVIGGVAGFSGGELLLAFFANGALLNFY
                     GFVPVALGGLGNNRGAILGGIALGLFQQAANFLVGGIFSSVAVFTLFVVVLLAAPQGL
                     FGAATVRRV"
     gene            2595..>2909
                     /locus_tag="VGA15_11945"
     CDS             2595..>2909
                     /locus_tag="VGA15_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588439.1"
                     /translation="MTAVTTALARGEATAFRALLPQLAPFLGIFAVAMILPFVSNDYW
                     ALIGTRAAIYWVLVSGLNLVVGFAGHLAIGYVALLTLGAYTTSVLVAGNVMPPVPVFA
                     ALP"
BASE COUNT          522 a          922 c          890 g          575 t
ORIGIN      
        1 aatttctagc aattagccgc tgcgacagcg attgcagtcg ggcaaacgcg tcgctgggaa
       61 aatttgtcgc acccgggata cgtccacatc aacagcgctt gaatagccct tggcctcccg
      121 tcactgctta atggtcgcga aggaccatac ttggactctc ccattgatcg aatcgagatt
      181 tccgcgttgg tcacgcaatg acgtgatacg ccatctgatg cggcttccct tccattgcgg
      241 ctggatcatc gtaatcgtga ggttcgtcgc catggcgacc ggcgtgaccg caggcgccct
      301 ctcacttcga gtccgtccgg ttctcaaaga aatttgcctt gttcgacgaa atcggcacgc
      361 cttttcccgc aatacgacgg cgattgacca gcccctctgg acaaggcgtc ctcatcaggt
      421 tcaaatttga attgcaaaac caagcttcgg ccaaaagccg aaaccgggat gcccgcggca
      481 aagacgggcg cccgcaaagg aaacaacggg agcatcacat ggccatttca cgtcgtgacg
      541 ttttattggg cagcgcgggt gcgctcggcg ctgcaacgtt ttcatttccg aaaccgtcga
      601 tagcccaatc ggagccgatc aagatcggct gccttgccgc gatgaccggc ccgagttcgg
      661 cgccgaccat cggcttcaac cgcggcgtca acttcgcggt ggaggccatc aacgccgccg
      721 gcggcgtcaa gggccgcaag atcgagatcg tcatgcgcga cacccagggc gaccccacca
      781 aggcggtgaa cgccacccag gaattgatca gccaggccaa ggtccacgcg atctgggggc
      841 cgctcaattc cggcgaggcg ctggccacca cgccgatcat ggcgcgcgcc aggattccgg
      901 atatccatcc ttgcgtggtc gaaagcctga tcgatcccgc gaaatttccc aatgcgttcc
      961 gcatggcgcc ttcgaacagc caatgggatg acgccgtccg caattattgc ctcaatatcc
     1021 tcaaggcgaa aaaggttgcc gtgatcggcg acaccacggg ctatggcgtc accgcgctcg
     1081 gcgcgtcggt ggcggcgttc aagaaggacg gcgccgaagt ggtctatcag gccaacatcg
     1141 attccaccca gcccgacatg acgccggaca tgctgcgcgc caaaaatgcc ggcgccgaag
     1201 tgatcgtggt ctggagcgtc tccaccggca tggaagcgcg aatgttcaac acccgcgcgg
     1261 cgatgaactg ggacgtctcg ttcgtcggtc acccgtccat gtcgtcaggc gaaatcgccg
     1321 gcctggtcga gaaacccgcc aactggaaaa aagtctacgc catcggctac aagagctgca
     1381 gttacgatgc cgcgggcaag ctgccgccga agagccagga tctcgtcgat cgtctcctca
     1441 aggccaacgt ggtgatcagc gatacgttgt tatggtggat cgccggcggc atcgatgcga
     1501 tcgagctgat cgccaagggc gtcgcggaaa gcggttccac cgacagcgaa ggcatcatca
     1561 aatactggaa tacgctgtcg aaatatccgg gctatttcgg cgactacacc ttcacgccga
     1621 cccagcacaa tggctatccg accgacgaga tcgtgatgtc ggaggcctct accgcaaaga
     1681 acggtacttt cgcggtggcg ccgggataca cttaggcggc ggcggagggc gcggccaatg
     1741 ctcgcctcga tactcgcatc cggattggcg gcgggcgcga tctacgcgct cgtcggcgtc
     1801 acctacaaca ccatgttctc gacttcgcgg gtgatgagct tcaccgccgg ccagctcgcc
     1861 atgctcggcg gcgtgttcgg ctcgatgttc acgctgaagc tggggatgcc cgttgtcgtc
     1921 ggtctcctat tgacgctgct cggatgcgcg atcatcggga tcatcaccga attcgtcgcg
     1981 gtgcggccgg tgctgaaaag cctcgaccag catctctatg tgctctccac gttggcgctg
     2041 gcgctgatga tccagcaggt ggcggcgatc aaatggggca ccgagccgca gccatttccc
     2101 cgcctgatcg gatggggcag tggggtgttt gacgaaaaat tctggctgcc ggtagtcgcc
     2161 tgcgcgatca ccatcatcgg tctcgaatac ctctatcgcc gcacgctggt gggccgcgcc
     2221 ttcctggcga tcgcggagga caatttcgcc gcgcgcgcgc tgggcctgcc cgagcgcaat
     2281 ctgcgcgtcg ccagttacgc gctcgccggg gtcatcggcg gggtcgccgg attttccggc
     2341 ggcgagctgc tgctggcgtt cttcgccaac ggcgcgctgt tgaatttcta tggcttcgtg
     2401 ccggtggcgc tcggtggcct cggcaacaac cgtggcgcca tcctcggcgg catcgcgctc
     2461 ggcctgttcc agcaggcggc gaactttctg gtcggcggca tcttctcgtc ggtggccgtg
     2521 ttcacgctgt tcgtcgtcgt tctgctggcg gcgccgcaag gcttgttcgg cgcggctacg
     2581 gtccggaggg tgtgatgacc gcagtcacca cggcccttgc acgcggcgaa gcgaccgcct
     2641 ttcgcgcgct gctgccgcag cttgcgccgt ttcttggaat ttttgcggtc gcgatgatcc
     2701 tgccgttcgt cagcaacgat tactgggcgc tgatcggcac gcgcgcggcc atctactggg
     2761 tgctggtctc cggcctcaac cttgtggtcg gcttcgccgg acatctggcc atcggctatg
     2821 tcgcgctgct gacgctcggc gcctacacca ccagcgtgct ggtcgccggc aatgtcatgc
     2881 cgccggtgcc ggtcttcgcg gcattgccg
//
LOCUS       DASWGM010000409         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_873346,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000409 DASWGM010000000
VERSION     DASWGM010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873346"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VGA15_11950"
     CDS             complement(<1..275)
                     /locus_tag="VGA15_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9588440.1"
                     /translation="MKRTPFRTILIANRGEIALRIMRTARRLGLGVVAVYSDADREAL
                     HVRAADQAIRIGGALPAQSYLRIEAIIAAAKAGGADAVHPGYGFLAE"
     gene            complement(287..1903)
                     /locus_tag="VGA15_11955"
     CDS             complement(287..1903)
                     /locus_tag="VGA15_11955"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX9588441.1"
                     /translation="MTFLESTIAPSSDAYKANRAGMLALIARMRGLEERTRAASAAAK
                     ERFHKRGQLLPRERVALVLDPGSPFIELSTLAGYMFDVPDADKSVPGGGVIAGIGFVS
                     GVRCMVSANDSGIDAGALQPYGLDKTLRVQELALENKLPYVQLVESAGANLLRYRVED
                     FVRGGNIFRNLALLSAAGLPVVTVTHGSSTAGGAYQTGLSDYIVMVRGRTRAFLAGPP
                     LLKAATGEIATEEELGGAEMHTSVSGLGDYLAENDRDALRIARDIMANLRWDRPGTDK
                     TPHRPPRHDPEELLGIMPMDHKRPVDMRQVIARIIDDSDFTEFGANYGPATVCGHARI
                     EGEAIGIITNNGPLDPAGANKATHFIQACCQSRTPLLYLNNTTGYMVGKAYEEAGMIK
                     HGSKMIQAVTSATVPQITIYCGASFGAGNYGMCGRGFHPRFCFSWPNAKTAVMGGEQA
                     AETMAIVTEAAAIRRGKPVEKEKLDAMKAQIIGVFDGQMDVFSTSARVLDDGVIDPRD
                     TRGVLSEVLAICREAEARNPQRMQFSVARP"
     gene            complement(2293..2499)
                     /locus_tag="VGA15_11960"
                     /pseudo
     CDS             complement(2293..2499)
                     /locus_tag="VGA15_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712796.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(2744..>3427)
                     /locus_tag="VGA15_11965"
     CDS             complement(2744..>3427)
                     /locus_tag="VGA15_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX9588442.1"
                     /translation="WEVSVSREIQQAIREACPGVIINHTTGTSGPNYAGALDCVRETR
                     PEMAACNAGSLNYLKVKSDNTWAWPPMMFDNAVEKVQAYLDVMKQVDTIPEFECFDVG
                     IVRCVGMYRQTGMYSGPLEYNFVMGVASGMPADPELLPILLKLKLPEAHFQVTAIGRA
                     EIWPLHQRCAELGGHLRTGLEDTFYQPDGAKVTSNGQLIESIAACARRAGREIASPAE
                     ARQIFGIRR"
BASE COUNT          613 a         1105 c         1057 g          652 t
ORIGIN      
        1 ttttccgcca gaaagccata gccgggatga actgcgtcgg ctccgccagc cttcgcggcg
       61 gcaatgatcg cctcgatccg caaataggat tgcgccggca gcgccccgcc gatgcggatg
      121 gcctggtcgg cggcccgcac atgcaacgcc tcgcggtccg catccgaata gaccgcgacg
      181 acgccaagcc caaggcggcg cgcggtacgc atgatgcgca gcgcgatctc gccgcgattg
      241 gcgatcagga tggtacggaa cggcgtgcgc ttcatcggcc catcgctcat ggccgcgcaa
      301 ccgaaaactg catgcgctgg gggttgcgcg cctcggcctc gcggcagatc gccagcactt
      361 ccgacagaac gccgcgggtg tcgcgcgggt cgatcacgcc atcgtcaagc acgcgcgcgc
      421 tggtcgaaaa cacatccatc tggccgtcga acacgccgat gatctgcgcc ttcatcgcat
      481 cgagcttttc cttctcgacc ggcttgccgc gccggatggc ggcagcttcg gtcacgatcg
      541 ccatggtttc ggcggcctgc tcgccgccca tgacagcggt cttggcgttg ggccatgaaa
      601 aacagaagcg cggatgaaac ccgcgcccgc acatgccgta attgccggcg ccgaacgacg
      661 cgccgcagta gattgtgatt tgcggcacgg tggcgctggt caccgcctgg atcatcttcg
      721 agccgtgctt gatcatgccg gcttcctcgt aggccttgcc gaccatgtag ccggtggtgt
      781 tgttgagata gagcagcggc gtccgcgact ggcagcaggc ctgaatgaaa tgcgtcgcct
      841 tgttggcgcc ggcgggatcg agcgggccgt tattggtgat gatgccgatc gcctctccct
      901 cgatgcgggc gtgcccgcac accgtggcag gaccgtaatt ggcgccgaac tcggtgaaat
      961 cggagtcgtc gatgattcgc gcgatcacct gacgcatatc gaccggccgc ttgtgatcca
     1021 tcggcatgat ccccaacagt tcctcgggat catgccgcgg cggacgatgc ggcgttttat
     1081 ctgtgcccgg ccggtcccat ctgagattgg ccatgatgtc gcgcgcgatg cggagcgcgt
     1141 cgcgatcgtt ctcggcgaga tagtcgccga gtccggaaac gctggtgtgc atctcggcgc
     1201 cgccgagttc ctcttcggtg gcgatttctc cggttgcggc cttcagcagc ggcgggcctg
     1261 cgagaaacgc gcgggtgcgg ccgcgcacca tcacgatgta atccgacaac ccggtctgat
     1321 aggcgccgcc cgccgtcgac gaaccatgcg tcaccgtgac caccggcaag ccggctgccg
     1381 acagcagcgc caggttgcga aagatattgc cgccgcgcac aaaatcctcg acgcggtagc
     1441 gcaacaggtt ggcgccggcg ctttcgacca gttgcacgta aggcagcttg ttctcaagcg
     1501 ccagttcctg cacccgcagc gtcttgtcga ggccgtaagg ctgcagcgcg ccggcatcga
     1561 taccagaatc atttgcgctg accatgcagc ggacgccgga aacaaatccg atgccggcga
     1621 tcaccccgcc gccgggcacg cttttgtcgg cgtcgggaac atcgaacatg taaccggcca
     1681 atgtagaaag ttcgatgaag ggcgagccgg gatcgagcac cagcgccaca cgctcgcgcg
     1741 gcagcaattg tccacgcttg tggaaccgct ccttggcggc ggccgaggct gcgcgcgttc
     1801 gttcttccag accgcgcatg cgagcgatca gcgccagcat gccggcgcga ttggctttgt
     1861 aggcatcgct gctcggggcg atcgtcgatt caaggaaggt cataaggatc aatcaccctc
     1921 gcgcaaacta caacaattgt cattccccga tgtgcaattg cacatcgtgg atgcgccacc
     1981 gaactcgggt ttacccgagt tcggcaatat cattatccaa gtcggctaca gccgacttgg
     2041 atgcgcaggc ccggaatcca tactccctgt cgtggttatg gattccgggc tcgcgctgac
     2101 gcgcgccccg gaatgacgtg gtgttgttgg cttactcggc agcattacgc actcttctcc
     2161 gcaaaatacc cggcaaaagc ctcgcgcagt tcgaccttgc gcagtttgcc ggtcgccgtc
     2221 atcggcattt catccatgat cctgatgaac ttcggcacct ggaagccgcc aaggtgcttg
     2281 gggcaatgct cggccgggtt ttaatccgcg cgcgaccaga taattggcaa agcggttggc
     2341 gtcgcgctcg atctcggtgt acgtcacgcg ccgctcgccg tcggtgacgg cagtccgatc
     2401 ggggaagcgg gccgccgcgc gtttcaacag gtcgccgatc gccacccggc cgatgcggcc
     2461 gggaccggac accccgccgg ttgcagatag atcctgcatt tcgtttcctc caaagcttca
     2521 acggcggcag cgcgctccct ctcccaccca actcgggttt acccgagttg ggcatctaat
     2581 gttgtcgaag tcggatatat ccgacttcga gtgggagaga gttggggtga ggggtcaggt
     2641 ctatcgatag gccgcaaccc ctcacccgga ttgcttcgca atccgacctc tcccacaagg
     2701 ggagaggtga ccgaactcaa ccgcctcgtt tcgtatccat gcactaacgc ctgatcccaa
     2761 agatctgccg cgcttccgcc gggctcgcga tctcgcggcc ggcgcggcgc gcacatgcgg
     2821 cgatgctctc gatcaactgg ccgttcgatg tcaccttcgc gccgtcgggc tgatagaagg
     2881 tatcttcaag cccggtgcgc aggtgaccgc cgagttcggc acagcgctga tgcagcggcc
     2941 agatttccgc tcgcccgatc gcggtgacct gaaagtgggc ttccggcaat ttcagcttca
     3001 agaggatcgg cagcaattcc ggatcggccg gcatgccgga ggcgacgccc atgacgaaat
     3061 tgtactcgag cggtccggag tacatgccgg tctggcggta catcccgacg cagcgcacga
     3121 tgccgacgtc gaagcattcg aactccggaa tcgtgtccac ttgcttcatg acgtcgagat
     3181 aggcctgcac cttctcgacg gcgttgtcga acatcatcgg cggccacgcc caggtgttgt
     3241 cgctcttcac tttaagataa ttcagcgagc cggcgttgca ggcggccatc tccggtctgg
     3301 tctcgcgcac gcaatcgagc gcgccggcat aattcgggcc cgaagtcccg gtggtgtgat
     3361 tgatgatgac gcccggacag gcctcgcgga tcgcctgctg gatttcgcgg gacacgctca
     3421 cctccca
//
LOCUS       DASWGM010000410         6841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1551286,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000410 DASWGM010000000
VERSION     DASWGM010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551286"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2520)
                     /locus_tag="VGA15_11970"
     CDS             complement(<1..2520)
                     /locus_tag="VGA15_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX9588443.1"
                     /translation="MIARLIDWSARNLMLVLIGAVFAAAAGVYSLQHSALDAIPDLSD
                     TQVIVYTEYQGQAPQVIEDQVTYPLTTAMLTVPKSKVVRGFSFFGVSFVYVIFEDGTD
                     IYWARSRVLEYLNAAARKLPSGVTPTLGPDATGVGWVYQYALQSKDKSLADLRSLQDW
                     TIRYGIAKAEGVAEVASVGGFVRQYNIILDPNRMRSLGIPLAKVRDAVRGSNMDVGGR
                     SVELSEFEFIVRGRGYVKSLSDLSNIVLKVDKGTPVLLKDVARVEFGPDERRGITELN
                     GEGEVASGIALQRFGQNALDVIDNVKARLADMASALPKGVEIIPVYDRSNLIHNAIET
                     LKRTLTEESIVVALVCIAFLLHVRSALVAIVMLPVGILMAFAAMKALGIGSNIMSLGG
                     IAIAIGAMIDGAIVMIENAHKHLERAPPDKPRLEVLIEAASQVGPALFFSLLIITVSF
                     MPIFTMESQEGRLFSPLAYTKTFSMAAAAILSITLVPALMVLFIRGKIIPEHKNPINR
                     FLIWVYRPLIRGVLKAKTLTIFLALVALGVSLWPARQLGTEFMPSLNEGTLMYMPTTL
                     PGLSVTKAAEILQTQNKIIKTFPEVASVYGKAGRAETATDPAPTEMFETIVNLKPKQQ
                     WRAGLTVDGLIAELDKALQFPGVSNAWTMPIKARTDMLATGIRTPIGIKVLGKDLTEM
                     EKLARQIETVVKAVPGTSSAFAERVIGGYYLDVVPDREALARYGLMVSDVQDTVSSAL
                     GGETITTTVEGRERYGVNLRYPRDLRSDPQSIARDVLVATPGGGTVPLGEVAAIKLTR
                     GPTSIRTENGQLAVYIFVDIRDRDLGGYVADAKAAVAASVQF"
     gene            complement(2517..3887)
                     /locus_tag="VGA15_11975"
     CDS             complement(2517..3887)
                     /locus_tag="VGA15_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9588444.1"
                     /translation="MVVGRSNGPLPGWLGAVLPAAAKQSPVQPEATGPIIYYRNPDGL
                     PDYSLTPKKTSAGKDYLAVRSSEDVSFEEKAPETMTAKGGDRGKIRFYRNPMGLPDTS
                     PTPKKDSMGMDYLPVYEGEQDDDSSVKVSVGKLQKAGVQTEVAERRTLNTVVRAPGTV
                     QEDERRKAVVSLRFEGFIDSVENVTTGTHVHKGQRLMRIYGPNLSSAAAEYLSALNAR
                     PDAGISNQALKGARRRLENLDAPEKFIADIERTREIPAYVSWPSPQDGEIVERAAVNG
                     MRAAPGDVLFRIVDHDVVWVIVDVAERDLSLIAVGQTVTVRPRAYPNRPFTGTVTLIY
                     PHLMAETRTARVRIELPNPDNVLRPDMYADVEIATGAEAPVLTVSNSAVIDSGERQIV
                     LLDKGDGRFEPRAVKLGRRGDGRVEIKEGLAENDKVVVSANFLIDAESNLKAALSGLN
                     QGEKSP"
     gene            complement(4108..5352)
                     /locus_tag="VGA15_11980"
     CDS             complement(4108..5352)
                     /locus_tag="VGA15_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9588445.1"
                     /translation="MISSVGITRLAAIGATTAALWLSPAAAGDQTLLGATVESVVAIA
                     KRLNPTVAAASLDFDAAVHKIGTAGVLADPTLILEAWDVNRQGVGQRRIGLEQEIKFW
                     SKYGLERDIAQSDADAAKFQGRATVTELIAQVIAAHGEYNAAYEAVAVSVEIKRRDDD
                     ILGLVRSRYGTTSVDQQDVIRAEVEAATAEGDVVRRQGELKSAAARLNALIGRPSLAP
                     LAAPTGFRAVKAISLAQVQALARSANPMLAMAGAQSNSAAQTKSLTDLNYYPDVTLGA
                     KYVQRPGTEDTGEFMLGVKLPLHYEVKDAEQRAAGARLGAAQARNEALRLRVDGQVAD
                     AWFSVDALRKVVQIYASRQVPAARASLDTARNGFQAGTTDLFFVFEAERRLRTVQLDL
                     LKLKVELQMKFAEMERLAGGSL"
     gene            complement(5349..5753)
                     /locus_tag="VGA15_11985"
     CDS             complement(5349..5753)
                     /locus_tag="VGA15_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX9588446.1"
                     /translation="MNSYTCARALQVALIGIAMTGSATLAPADINDYEFQLVDKTVKK
                     GDAIISVRLVHKPDGKAVSDAVIFAKRLDMAPDGMETMKTTIEPMPSTEPGIYKFKAA
                     VTMEGGWQLSLAAKVQGETGTVENKLVIRATP"
     gene            complement(5850..6206)
                     /locus_tag="VGA15_11990"
     CDS             complement(5850..6206)
                     /locus_tag="VGA15_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588447.1"
                     /translation="MNLLRLIGRLFAVFAIVGLVAAPLVTPVAAKRLSVGEMSDMAAT
                     PGDMPCCPDSQKSKSCQDCPLVATCMLTIAQAEPSATNGIQVFFQTRRLSFGLVDLIV
                     DGLIGDPPDHPPRILT"
     gene            complement(6328..6813)
                     /locus_tag="VGA15_11995"
     CDS             complement(6328..6813)
                     /locus_tag="VGA15_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEX9588448.1"
                     /translation="MHMSDTLRSVTRRSALALFAAFLALQRSPAEAEFPAILVHKDPN
                     CSCCAGWVRHLKDAGFAVRVEETTNLQPVRKRLGVPADLAACHTAEVDGYVLEGHVPA
                     AAVRRLLEQRPTAVGLAVPGMPAGSPGMEGGTPQRYDVVLFGPDGRQSFMRFVGSESV
                     R"
BASE COUNT         1348 a         2133 c         2026 g         1334 t
ORIGIN      
        1 aaactgcacg ctggcggcga cggccgcctt ggcgtcggcc acgtagccgc cgagatcgcg
       61 gtcgcggata tccacgaaaa tgtagacggc gagttggccg ttctcggtcc ggatcgaggt
      121 cgggccgcgc gtcagcttga tcgcggcgac ctcaccaagc ggcacggttc caccgccggg
      181 cgttgccacc agcacgtctc gcgcaatcga ttgcggatcg ctgcgtagat cgcgcggata
      241 gcgcagattg acgccatagc gctcgcgacc ctcgacagtg gtggtgatgg tctcgccgcc
      301 gagggcggac gagacggtat cttgcacatc gctgaccatg agaccgtagc gggcaagcgc
      361 ttcccggtcg ggtacgacat ccaggtagta gccgccgatg acacgttcgg caaaggcgct
      421 cgaggtgccc ggaactgcct tcacaaccgt ttcgatctgc cgtgccagct tctccatctc
      481 cgtcaggtct tttccaagaa ccttgatgcc gatcggcgtg cgaattccgg tcgcaagcat
      541 gtcggtgcgg gccttgatcg gcatggtcca ggcgttggaa acgccgggaa actgcaaagc
      601 cttgtccagc tcggcgatca ggccatcgac ggtcaggccg gcacgccatt gctgcttcgg
      661 tttgagattg acgatcgtct caaacatctc ggtcggcgca ggatcggtcg cggtctcggc
      721 gcgtccagcc ttgccgtaaa ccgaggccac ttccggaaac gtcttgatga tcttgttctg
      781 ggtttgcagg atttccgccg ccttggtcac cgacagaccc ggcagcgtcg tcggcatata
      841 catcagcgtg ccttcgttga ggctcggcat gaactcggtt ccgagctgcc gggctggcca
      901 cagactcacc cccaaggcga caagcgcgag aaagatcgtc agggtcttgg cctttagcac
      961 cccacggatc agcggccggt agacccagat caggaaccga ttgatcggat tcttgtgctc
     1021 cggaatgatc ttgccgcgaa tgaacaacac catgagagcc ggaaccagag taatcgacag
     1081 gatcgccgcc gcggccatcg agaacgtctt ggtgtaggcc agcggactga acaggcgccc
     1141 ctcctgcgat tccatggtga agatcggcat gaacgacacc gtgatgatca acaggctgaa
     1201 aaacagcgcg ggaccgacct ggctcgccgc ttcgatcagg acttcgaggc gcggcttatc
     1261 gggcggtgcg cgttcgagat gcttgtgagc gttctcgatc atgacgatgg cgccgtcgat
     1321 catggcaccg atcgcaatcg cgatgccgcc gaggctcatg atgttcgaac cgatgccgag
     1381 cgccttcatg gcggcgaaag ccatcaggat gccgaccggc aacatcacga tcgcaaccaa
     1441 cgcactgcgc acgtgcagca ggaacgcgat gcagacgagc gcaacgacga tactttcttc
     1501 cgtgagtgtc cgcttcagtg tctcgatcgc attgtgaatc agattggagc ggtcatagac
     1561 cgggatgatc tcgacgcctt tcggcagggc cgacgccata tcggcaagcc gcgccttcac
     1621 attgtcgata acgtcaagcg cgttctggcc gaaacgttgc agagcgatac cgctggcgac
     1681 ctcgccctcc ccgttgagct cggtaatacc tcggcgctcg tccggcccga actcgacccg
     1741 tgccacatcc ttcagcaaga ccggcgtgcc cttgtcgact ttcaacacga tattcgatag
     1801 atcggaaagg cttttgacat aaccgcgtcc gcgcacgatg aattcgaatt ccgacaactc
     1861 cacgctacgt ccgccgacat ccatgttgct gccgcggacc gcatcgcgca ctttcgcaag
     1921 cgggataccc agcgaccgca tccggttcgg atcgaggatg atgttgtatt gcctgacgaa
     1981 gccgccgacg cttgccactt cggcgacgcc ctctgccttg gcaatgccgt agcgtatggt
     2041 ccagtcctgc aaggacctga gatcggccag cgatttgtcc ttggactgca gcgcatactg
     2101 gtaaacccag ccaacgccgg ttgcatccgg ccccagcgta ggcgtcacgc cggacgggag
     2161 cttgcgcgct gcggcgttga ggtattcgag gacacgcgag cgcgcccaat aaatatccgt
     2221 accatcctcg aaaatgacgt aaacgaacga cacgccgaag aaggaaaagc cgcgcaccac
     2281 ctttgatttc ggcacggtca gcatcgccgt cgtcagcgga taggtcacct ggtcctcgat
     2341 gacttgcggt gcctgaccct ggtattcggt atagacgatg acctgggtgt cggagagatc
     2401 tggaatggcg tcaagcgccg agtgttgcag cgaatagacc ccggctgccg cagcgaacac
     2461 cgcaccgatc aggaccagca tcaggttgcg agccgaccag tcaatgagcc gcgcgatcac
     2521 ggcgacttct ccccttggtt aaggccgctg agcgcggctt tgagattact ttcggcatcg
     2581 atcaggaaat tcgcggagac gacgaccttg tcgttttcag cgagcccttc ctttatctcg
     2641 acgcgaccgt cgccgcggcg ccccagcttg actgcgcgtg gttcaaaccg gccgtcaccc
     2701 ttgtcgagca gcacaatctg ccgctccccg ctgtcaatga cagcgctgtt ggacaccgtc
     2761 agaaccggag cctcggcccc ggtggcgatc tcgacatcgg catacatgtc ggggcgcaac
     2821 acattgtccg gatttggcaa ttcgattctt acccgcgcgg ttcgcgtctc cgccatcaag
     2881 tgcggataga tcaacgtgac tgtcccggtg aaaggacggt tcggataggc acgcggccgc
     2941 acggtgaccg tttggccgac cgcaatgagc gaaagatcgc gctcggcaac atccacgatc
     3001 acccaaacga cgtcatgatc gacgatacgg aacagaacat cgcctggggc cgcacgcatg
     3061 ccattaacgg cggctcgttc aacgatctcg ccgtcctgcg gcgaaggcca gctcacatag
     3121 gccgggattt cgcgcgtgcg ctcgatgtcg gcaataaact tctcgggggc atccagattt
     3181 tcaaggcgcc gacgtgcgcc tttcaaagct tgattgctga tgccggcgtc cgggcgggca
     3241 ttgagcgctg acagatattc ggcggcggcg ctcgagaggt tcgggccgta gatgcgcatc
     3301 agccgctggc ccttatggac atgcgtgccg gtggtgacgt tttcgacgct gtcgatgaaa
     3361 ccttcgaaac gcagcgatac gaccgctttg cggcgctcgt cttcctgaac ggtgcccggt
     3421 gcacgaacaa cggtgttcag agtgcggcgt tccgcaacct cggtctgaac acctgccttt
     3481 tgcagcttgc cgacactgac cttcaccgac gaatcgtcgt cctgttcgcc ctcatagacc
     3541 ggcaaatagt ccatgcccat cgaatccttc ttcggggtcg gcgacgtgtc gggaaggccc
     3601 atagggttgc ggtaaaagcg aattttgcct cgatccccac ccttcgcggt catcgtttcc
     3661 ggagctttct cctcgaaact cacatcttcg ctactgcgga ctgcgagata gtctttgccc
     3721 gccgaagtct tcttcggagt cagcgaatag tccggcaggc cgtccggatt acggtagtag
     3781 atgatgggcc ccgtcgcttc tggctgaact gggctctgtt ttgcggccgc gggcagaaca
     3841 gcgccaagcc agccgggaag cggaccgttc gagcggccga cgaccaatcc gaccgctccg
     3901 gcagccagaa tcgcggacac aatcaagaaa gcggctatta gccgtctcat agcaaatctc
     3961 ccgccaagcg ctccatctca gcgcgtttga atcaggtagg cgcttgcgca cgagacgtgc
     4021 atcatctgcg cttccccggt gagttcgggg caaatcgcca gccggttaca tgctcacgcc
     4081 gacgtgaagg cgcccaatac gcctctctca cagcgaacct cccgccaaac gttccatctc
     4141 ggcaaatttc atctggagct cgactttgag tttcagcaga tcgagctgaa cggtccggag
     4201 ccggcgctcc gcttcgaata caaaaaacag atcggtcgtt ccggcctgaa agccatttcg
     4261 tgcggtatcg agtgaagcgc gcgccgcggg gacttggcgc gacgcgtaga tctgcacgac
     4321 cttccgcagc gcatcgacgc tgaaccaggc atcggcgacc tgtccatcga cccgcagccg
     4381 cagcgcctcg ttgcgggctt gcgccgcgcc aagacgcgcg cccgccgcac gttgctcggc
     4441 gtccttgacc tcatagtgaa ggggcagctt gacgcccagc atgaactcgc cggtgtcctc
     4501 cgtgccgggc cgctgcacgt atttggcgcc gagcgtcacg tcggggtagt agttgagatc
     4561 ggtaagggat ttggtttggg ccgcggaatt gctttgcgcc ccggccatcg ccagcatcgg
     4621 gttcgcggag cgcgcgagcg cctgcacctg cgcgaggctg atcgccttga cggcccgaaa
     4681 gccggtcggc gcagccaagg gcgccaggga cggacgcccg atcagggcat tgagccgcgc
     4741 tgcggcagat ttcagctccc cctgccggcg aacgacgtcg ccctcggccg tggcagcttc
     4801 cacctcggct ctgatgacgt cctgctgatc gaccgacgtt gtgccgtatc gtgaccgcac
     4861 caatccgaga atatcgtcat cgcgccgttt gatttcgaca gagactgcga cggcttcgta
     4921 cgccgcattg tattcgccat gcgcggcaat gacctgcgca atcagttcgg taacggtcgc
     4981 gcgtccctgg aattttgcgg cgtcggcatc ggattgcgcg atatcgcgct cgaggccgta
     5041 tttgctccag aacttgattt cctgttcaag gccgatgcga cgctggccaa cgccttgccg
     5101 gttgacgtcc cacgcttcga gaatgagggt cggatcggcc aaaacgccgg ccgttccgat
     5161 cttgtggacc gcagcatcga agtcgagcga ggccgccgcc accgtcggat tgagacgctt
     5221 ggcgattgcg acgacgcttt cgaccgtcgc accgagcaga gtctgatcgc ctgccgcagc
     5281 tggagacaac catagcgctg ctgtggttgc tccaattgcg gcgagccggg tgattccgac
     5341 agagctgatc acggcgttgc cctgatgacg agtttattct cgaccgtgcc cgtttcgccc
     5401 tgaactttgg ctgccaagga caattgccag ccgccctcca tcgtcacggc agccttgaac
     5461 ttatagattc caggttccgt gctcggcatg ggttcgatcg ttgtcttcat cgtctccatg
     5521 ccgtccggcg ccatatccag tctcttggcg aagatcaccg catcggagac ggctttgccg
     5581 tcgggcttat ggacgagtcg gaccgaaatg atcgcgtcgc ccttcttgac ggtcttatca
     5641 accagttgga actcatagtc attgatgtcg gcgggcgcga gcgttgccga cccggtcatg
     5701 gcaatgccga tcagcgcaac ctggagcgcg cgtgcgcaag tgtaactatt catatgcaat
     5761 cctcggtgtc ctgacgtgcc gcgacggcgg atgcattgtt ctgcgcggcc ctaggaccgg
     5821 cgcagcagca gacggcatga taatgccgat caggtcagga ttcgaggagg atggtccgga
     5881 ggatcgccaa tgaggccatc aacgatcagg tcgacgagac cgaaggacag tctacgggtt
     5941 tggaagaaaa cctggatacc gtttgtcgcg gacggttcag cctgagcgat cgtcaacatg
     6001 caggttgcga cgagcgggca gtcctggcag ctcttacttt tctgactgtc cggacagcac
     6061 ggcatgtccc ccggcgtagc ggccatatcg ctcatctcgc cgacagatag ccgcttcgcc
     6121 gcaaccggtg ttaccagtgg cgcagcaacc agccccacaa tcgcaaagac cgcgaacagc
     6181 cgaccgatga gtcggagaag attcatgact taacatcaca caaggcgggg aactttggaa
     6241 gcgcacttaa tttcacaatt ttgccagacg atccgcgtca gaaaaaaacc ttgaaagtgc
     6301 atacggttaa ccggaattcc gagcgcctta tctgacactc tccgagccga caaaccgcat
     6361 aaaggattgt cggccatccg ggccaaagag aacgacgtca tagcgctgag gtgtcccccc
     6421 ttccattccc ggcgatccgg cgggcatccc ggggacggct aatccaacag cggtcggccg
     6481 ctgttccagg agtcgtcgca cggccgccgc cgggacatgt ccctccagca catatccatc
     6541 gacctcagcg gtgtggcaag ccgcgagatc ggccggcacc cccagtcgtt tcctgacggg
     6601 ctgaagattt gtggtctcct caaccctaac agcgaagccc gcgtccttga gatgtcgcac
     6661 ccaaccggcg cagcaactgc aatttggatc cttatgaacc aggatcgccg ggaactcggc
     6721 ttcagcggga gaccgttgca aagcgagaaa agcggcgaac aaagctagcg cgcttcgacg
     6781 cgtcacgctg cgcagtgtgt ccgacatgtg cattctccgt gagaggccct gattgtccga
     6841 a
//
LOCUS       DASWGM010000411         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2538486,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000411 DASWGM010000000
VERSION     DASWGM010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538486"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VGA15_12000"
     CDS             complement(<1..371)
                     /locus_tag="VGA15_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588449.1"
                     /translation="MALIGRLFVILFGFLAACLVAGMIVVGAVLFPEFSDLGTGPIDP
                     GALNIVLGFGFIFVSGFALLPAMIVAAITEAFHIRSALAYAVGGGVVGLACYLGLVPF
                     DPATLRFDGIVRRHLEIMTGAG"
     gene            complement(563..1774)
                     /gene="rlmN"
                     /locus_tag="VGA15_12005"
     CDS             complement(563..1774)
                     /gene="rlmN"
                     /locus_tag="VGA15_12005"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX9588450.1"
                     /translation="MQTILETPDAPAPLEKVALETYVPPAKPSLIGLSRAEIAERLGA
                     IGVAPAQRKMRGQQLWHWIYVRGAKNFDEMTSISKEMRAQLEAHFTVARPEVAAEQIS
                     NDGTRKWLLRLPSGDSLEKAHEVECVYIPETDRGTLCVSSQVGCTLNCSFCHTGTQRL
                     VRNLTAGEIVGQIMVARDRLNDWADRETPSGGRLVTNVVMMGMGEPLYNFDAVRDALL
                     IVSDNEGIGISRRRITLSTSGVVPNIVRTGEEIGVMLAVSLHAVRDELRNELVPLNRK
                     YPIAELLQACRDYPGSSNARRITFEYVMLKGVNDSMEDAKLLVKMLKGIPAKINLIPF
                     NPWPGTRYECSDWDQIERFSEYIFNAGYSSPVRTPRGRDILAACGQLKSETEKLSVRE
                     RQALRAMAMTD"
     gene            complement(1931..>2197)
                     /locus_tag="VGA15_12010"
     CDS             complement(1931..>2197)
                     /locus_tag="VGA15_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX9588451.1"
                     /translation="VSIMIGYNLKPGSESTLEVGGASYAMYTQGDGLWIKNAAEEERM
                     VDALRKASDAVVKGVSAKGTETTDTFSLKGLSQALDKLAQDCKR"
BASE COUNT          443 a          688 c          647 g          419 t
ORIGIN      
        1 cccgcgccgg tcatgatttc gagatgacgg cggacgatgc cgtcgaagcg aagcgttgcg
       61 ggatcgaacg gcaccaggcc gagatagcag gccagaccaa ccacgccgcc gccgaccgca
      121 taggccaatg cgctcctgat atgaaacgct tcggtgatgg cggccacgat catcgccggc
      181 agcaaggcaa aaccgctgac aaagatgaag ccgaaaccga gcacgatgtt cagcgcgcca
      241 ggatcaatcg gaccggtgcc gagatcgctg aattccggaa acagcaccgc gccgaccacg
      301 atcatccccg ccaccagaca tgcggcgaga aatccgaaca gaatgacgaa cagccggccg
      361 atcagcgcca tgcagctaag tccattgcga acacgagcac aaacctctca accgtcatgc
      421 ccggccagag cagtctgctt ttgcgcagac tgcgtaaact tgtctgcgat gccgggcatc
      481 cacgtcttcc ttggtgttgc aacaaaataa gacgtggatg gccgggacaa gcccggccat
      541 gacgaacttt accttgtgtg tcctaatccg tcatcgccat ggcgcgcaac gcctgccgct
      601 cgcggaccga taatttttcg gtctcggatt tcaattggcc gcaggcggcc aggatgtcgc
      661 ggccgcgcgg ggtgcgcacc ggcgaggaat agccggcgtt gaaaatatat tccgagaacc
      721 tttcgatctg gtcccagtcc gagcattcgt agcgcgtccc cggccacgga ttgaatggaa
      781 tcaggttgat cttggctgga atgcccttca gcatcttcac caggagtttc gcgtcttcca
      841 ttgaatcgtt gacgcccttc agcatcacat attcgaaggt gatgcgccgc gcattggagg
      901 agccgggata atcgcgacag gcctgcagca attcggcaat cggatatttc cggttcagcg
      961 gcaccagttc gttgcgcaac tcgtcgcgca ccgcgtgcag cgagaccgcc agcatcacgc
     1021 cgatttcttc cccggtgcgg acgatgttcg gcaccacgcc cgaggttgac agcgtgatgc
     1081 ggcggcggga aatgccgatg ccttcattat cggaaacaat cagcagcgca tcgcgcaccg
     1141 cgtcgaaatt atacaagggc tcgcccatgc ccatcatcac cacgttggtg acgagacggc
     1201 cgccggaggg cgtttcgcgg tcggcccagt cgttgaggcg atcgcgcgcc accatgatct
     1261 ggccgacgat ttcacctgcg gtgagattgc gcaccagccg ctgcgtgccg gtgtggcaga
     1321 atgagcaatt cagcgtgcaa ccgacctgcg aggaaacgca cagcgtgccg cgatcggttt
     1381 ccgggatata aacgcactcg acttcatgcg ccttctcgag cgaatcgccg ctcggcaagc
     1441 gtaatagcca tttgcgggtg ccgtcgttgg agatttgttc ggcggcgacc tcgggacgcg
     1501 cgaccgtgaa atgcgcctca agctgcgcgc gcatttcctt ggagatgctg gtcatctcgt
     1561 cgaaattttt cgcaccgcgg acgtagatcc agtgccagag ctgttgccca cgcatcttgc
     1621 gctgcgccgg cgccacgccg atcgccccta accgttccgc gatctcggcg cgtgacagtc
     1681 cgatcagcga cggctttgcc ggcggcacat aggtttcgag cgcgaccttt tccaagggcg
     1741 ccggggcatc aggcgtttcg aggattgtct gcattgcctg gtatctcatt gagaccgtca
     1801 tgcccggcct cgtgccgggc atccacgtct tttcagcggc tggtcaggac gtggatggcc
     1861 gggacaagcc cggccacgac gactaataac gttcccttat atagcaaccg gcaaagcaat
     1921 tgcgaccaag ttagcgcttg caatcctgcg ccagtttatc gagggcctgg gaaaggccct
     1981 tcagcgagaa cgtgtccgtg gtctcggtgc ccttcgccga gacgcccttg accaccgcgt
     2041 cggaggcctt gcgcagggca tccaccatcc gctcttcctc ggcggcattc ttgatccaga
     2101 ggccgtcgcc ctgggtgtac atcgcgtagg aagcgccgcc cacctccagc gtcgattccg
     2161 agcccggctt caggttgtag ccgatcatga tggaaac
//
LOCUS       DASWGM010000412         9811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2538490,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000412 DASWGM010000000
VERSION     DASWGM010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538490"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="VGA15_12015"
     CDS             <1..96
                     /locus_tag="VGA15_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEX9588452.1"
                     /translation="AKDWENLNRKALAFLRLASIRLMLRKLCNPA"
     gene            complement(294..1017)
                     /locus_tag="VGA15_12020"
                     /pseudo
     CDS             complement(294..1017)
                     /locus_tag="VGA15_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648376.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            1246..1416
                     /locus_tag="VGA15_12025"
     CDS             1246..1416
                     /locus_tag="VGA15_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588453.1"
                     /translation="MPLIKVPIENVFNPEQKHQMIQLVIDAIVSIEGENMRGGSRFRK
                     SRAANGASAASL"
     gene            1698..2219
                     /locus_tag="VGA15_12030"
     CDS             1698..2219
                     /locus_tag="VGA15_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX9588454.1"
                     /translation="MKAIVGAGFAIFASLFVVTGIATAQPASMGCSPDNSASTSQTLR
                     CEGGFTIVVENGARFTLLDRDGNGRVDSVELSGKALLLEVPEKPGGNHFEVMTPQAIA
                     AVRGTKWAVDAAEGKTSVFVVNGRVSVARRVGANRVVLGPGEGVDVEATGALTVKRWP
                     PARVAALLARLAR"
     gene            2267..4081
                     /locus_tag="VGA15_12035"
     CDS             2267..4081
                     /locus_tag="VGA15_12035"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9588455.1"
                     /translation="MSGLWGAAVYFVHERGRLGFLDRIETAMTDLRTLARGVRFPPDF
                     VTIVAIDDAMVYPLARTDLARIIDAIARLEPKVIAVDLLLVDRGTNDGDEALEKSLMG
                     RASVLAAAAVFAQASQSIAAEKDEPLSGLPKAEKFLLPLKRFSDHAEIGIVNVTTGRS
                     GTPSSIPMLFRTHDRIEISFSLRAAALAIGKEPTIKPDRVVFGERSVATDVDHTLPVT
                     FYGPRRTIRTISAASVLAGEIAADAIHDRVVVVGATVTGGGDFFPTPFDPVMPGVEVT
                     STAITHLLAGDGILRNQSVRLVDGIIAVMLPMILVTLLAWRRSAIGPIATLIVVLTFA
                     TANVLVFSQGIWLSAALPIAATAPPAILFAAVQLWSGRRRAQYFATRSKLLGQFQAPD
                     IQEWLTRDPGFLKEPIRQNAAVIFIDLSGFTSLSERLGPDPIRDLLKEFHALVDKEAV
                     SSGGMIPSFLGDGAMILFGLPQATPDDALRAAQCATGLCVSAERWLASLPQPINSCLG
                     FKVGAHFGTIVASRLGGGSYHHITATGDTVNVANRLMEVAAGHEVELAVSDELLHAAG
                     RNSSLFDVGVLTGPIETRLRGRSGSLAVWFWRVSSGTS"
     gene            4296..5705
                     /gene="leuC"
                     /locus_tag="VGA15_12040"
     CDS             4296..5705
                     /gene="leuC"
                     /locus_tag="VGA15_12040"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9588456.1"
                     /translation="MEKPGRTLLSKIWDQRVIAHIGDDTDLLHVDRHLLHDLGGSRGL
                     LDLKSRKLSVHNPELTFATPDHAISTARGRAGTSKIGQELLAGLRTETSAVGIEMFDI
                     DEPGQGIVHVIGPELGLSLPGCLIVCGDSHTCTHGGLGALAFGIGSSELTHVLATQAI
                     IQRRPKTMRVSFEGRLPFGVTAKDLILALIGHIGAAGGTGYAVEYAGSAIRDLPIEGR
                     LTICNLSIELGAKMGLIAPDEKTYEYLRGRTYAPQGEMWQRAVEAWRQLPSDADAVFD
                     REVVIDVEAIIPQVTWGISPEHVIGVDGHVPDPQQIADPARRAAVETALDYMGLQPGA
                     PIIGTPVDWVFIGSCANSRLSDLRAAAEVARGRKVAPGVRAWVVPGSETVKRDAVAEG
                     LDKLFTDAGFEWREPGCSMCLAANGETVSPGQRSVSTSNRNFIGRQGPRARTHLASPA
                     TAAATAISGAIADVRMMEH"
     gene            5707..6342
                     /gene="leuD"
                     /locus_tag="VGA15_12045"
     CDS             5707..6342
                     /gene="leuD"
                     /locus_tag="VGA15_12045"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX9588457.1"
                     /translation="MAKPFTKLAAIAAPIMRSNIDTDVIIRIDRLVGNSVRGTLGKWA
                     FGALRYLPDGSENPEFILNREPYRQAEILVTGPNFGCGSSREGAVWSLQEMGIRAIIG
                     SGFGDIFFANCFQNGILPIVVDKEIVDGLAVEVEATQGAGRVSVDLREQTITAPSGKR
                     HSFEIDPRRRTGLLEGLDEVSLTLQRDDEIRAFQVSDRVERPWIHFARKSA"
     gene            6339..7478
                     /gene="leuB"
                     /locus_tag="VGA15_12050"
     CDS             6339..7478
                     /gene="leuB"
                     /locus_tag="VGA15_12050"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX9588458.1"
                     /translation="MNNLSNMIKVAVVGGEGIGPEVTAQSHRILNWFTGRRGVPMILR
                     EAQYGLIPYLATGRVSPEDTAEAMDEADAIVWGATGGPETKEVPAAARKAGSLLSLRS
                     KYDLYANLRPIVANPALAESAPLKASVLKGVDFVIIRELTSGIYFGEPRGIETLADGQ
                     RRGFNTEQYTTNQIRRVARAAFELARTRKGHVCSVDKANVLETSALWREEVITLHAAG
                     FSDIELSHLYVDNAAMQIVRQPSQFDVMVTGNIFGDILSDCAAMASGSLGMLPSASLG
                     PVDRFGRRKALYEPVHGSAPDIAGKGIANPLGSILSVAMMLRMTLNRPDDADLLEKAV
                     DIALAAGARTADIAEPGATKLSTREMGDAVLDALEQVVSKQTEPA"
     gene            7478..8527
                     /locus_tag="VGA15_12055"
     CDS             7478..8527
                     /locus_tag="VGA15_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588459.1"
                     /translation="MTRHFTRRSALTIVAGGGASVAAGRAWAEDKSGRVIYPVAVPVY
                     QTQFVADRIGYFKEAGLDCKLIQGGSGVKTREIIASSQGDIGIGDITHPMQLSNHGRN
                     GRVLMPVDTRSSSVMFIIRKDLKDQGITTLEALTEWKRPDGRKPIISVSSLGGTNHVW
                     ASYYMETMGLDQKVTWIGTGNVDTMLGSLKTKQVDVLVSSLSLLNESAEQGWGALLFD
                     GTDEAIWNKYIGGKVPVTSHFTLQSTIDKDPPKMQAFVTALWRATQWIKMHSPDEIYD
                     SIEPYVGSTSRAANIFEITALQKVADYEATIDAAGFARGEKVWFREMTGIKPLTIADV
                     VSPTFIEVARKAYPG"
     gene            8539..9351
                     /locus_tag="VGA15_12060"
     CDS             8539..9351
                     /locus_tag="VGA15_12060"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588460.1"
                     /translation="MLTPPHRQDVQRIDVRGLSKSFQLAGTAIEAVRDVSFSVHRGEF
                     VALLGPSGSGKSTILNMIATLIKPSSGQILIDGAPVIPGKATPNVGYVFQRDTLFPWR
                     TVADNIGYGLQLAGVPEVVRKERISASVAQAGLQGFEQAYPSALSGGMRQRAALMRTL
                     VVEPQILLMDEPFGALDTHTKIDMHDVLLRIWEREQQTVLFVTHDLGEALTLADRIIL
                     FSARPGMIKDTFEVDFARPRDAVKVRETPRYAELFQHIWHSLGEEFVKGRSS"
     gene            9369..>9811
                     /locus_tag="VGA15_12065"
     CDS             9369..>9811
                     /locus_tag="VGA15_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588461.1"
                     /translation="MSTWAWQALICVAVLSIWQWGYDLHAIVPWLVPDLFDPYFVSKP
                     SEIFQHFLILSCFKSKLGAVNGWFNDDFAKCMARNENNLWIATAITLKNTFFGFVTGV
                     SSGFVVGLILGRSDRLSAIFQPFITAVNSIPRIALAPIIVLAFGIG"
BASE COUNT         1928 a         2838 c         3056 g         1989 t
ORIGIN      
        1 gccaaggatt gggagaatct caaccgaaag gcgctcgcgt tcttgcgcct cgcctcaatc
       61 cgcctcatgc tcagaaaact ctgcaatccg gcctgaagtc tccggacgga ctctaagagt
      121 gcagccctgg cgacatcgtg ctttccagcc atgcccgcgg cggcggtcag cacacgatgc
      181 ccaccgacga aatcgccgcg ctgccttcgc catcggctcg aaccgccgat agcaccgcgc
      241 gctgggcgat cggcgccacc gttgacatat ccgcccagcc cccatgggcg ccgaaaaacg
      301 tcggcaaggt cgcggtcgta acgttccgcc ccagatgtgg cttccggtcg aaacatcgtt
      361 gagctgcgcc atgatccgca cgcgatcacc gcttttgcgc acgctgccct cgaccacata
      421 gccgatccca agctcctcgg cgacctgctc catgtgcacg gccttgccct tgcagatgaa
      481 tgaagagttg cgagcgatca cgaagaacca gcgcagcttg gacagcgccg tgatgatgtc
      541 ctcgctgatc ccatcggaaa aatattcctg ttccggatca ccgctcatgt tggtgaaggg
      601 gagcactgca atggcggggc ggtctggcaa cagcaatgct ggccgcgaca cgtcgcggat
      661 ttcgtccggc ggcggatcgg tggcttggtt cggcgtgttg tcgtcaactt gcgtctgcac
      721 ggcaccgatg aagcgcagac ccttgcgtgg gatggtgcgg atcagctgct gcttccggcc
      781 gctgtcgccg accgcatttc gtgccgcgtt gatacggctg gtcagcgtcg attccgatac
      841 aattcgaccg ccccagaccg aagcgatcag atcatccttg ctgacgacac ggtcgcggtt
      901 ttgtactagg tagaccaaaa ggtcaaacac ctgcggttcg acgtgaacgg cgcgctttgc
      961 gcgccacaat tcgcgccgtt caagatcgat ctcgcagtcg ccaaagccga gggtcatccg
     1021 tggttccaga gcattcggca acggccaaag tcagctgcta cccgcaaaag gattagcatt
     1081 tccagctagc ccccgggtgc gacgagcgct gaaagcaggg ttatccgaag cgcggctcaa
     1141 gccggtccga agaataaatc acaattctct caaggtcttg gccaaccgca acgggtatgc
     1201 tgctgacata tcgctgaggc gaggctacca tttaggagaa cggcaatgcc attgataaaa
     1261 gttcccatcg agaacgtgtt taatcccgag caaaagcatc aaatgattca actagtgatc
     1321 gacgcaatag tgtcgatcga aggcgagaac atgcgcggcg ggtcaagatt ccggaagtcg
     1381 cgagcggcga atggagcatc ggcggccagc ctctgaccac ctagggggtc aaggatctcg
     1441 cggccggacg aaaggcggcg tgaggccccg tccgcggtct ggcatctccc cacaggttgc
     1501 ttacccacct gtgacgcacg ctcgggcgtt acgggcagcc tgctgcaagg tgccgcagcc
     1561 attgagcgat gcgcagatag cggcgtcggc ggcgccgcta ttattttttg ttactttcag
     1621 caccgcaaac tggcagaata gccgcgctag acgatcccaa atctgcatct tcgaaatcgg
     1681 gatcaagagg agttgccatg aaggcgattg ttggcgcggg attcgcgata ttcgcctctt
     1741 tatttgttgt caccggcatc gccacggcgc agcccgccag tatggggtgt tcgccggaca
     1801 attcagcgag cacatcgcag accctgcgat gtgaaggtgg cttcaccatc gtcgtcgaaa
     1861 atggcgcacg atttaccctt ctggaccgcg atgggaatgg ccgcgtggat tcggtcgaac
     1921 tgagcggcaa ggctttgcta ctcgaggttc cagaaaagcc aggcggtaat cacttcgagg
     1981 tgatgacgcc gcaagcgatt gctgcggtgc gcggcacgaa atgggcggtg gatgccgcgg
     2041 aaggcaagac gtcggttttt gtggtcaacg gacgggtgag cgtggcgcgg cgcgtcggtg
     2101 ccaatcgtgt cgtactcggg cccggcgagg gcgtcgatgt ggaagcgaca ggtgccctga
     2161 ccgtgaagcg ctggccaccc gcccgcgtcg ccgcattgct ggcgcggctt gcacgataat
     2221 acagcgaatg ggtagtcgac atctccagat agtgattgct gtgttcatga gcggcctttg
     2281 gggcgcggcc gtgtatttcg tacacgagcg tggtcgcctg ggctttctcg atcgcatcga
     2341 gaccgccatg accgatcttc gaacgttggc tcgcggcgta cggtttccgc ctgacttcgt
     2401 taccatcgtc gcgatcgacg atgccatggt ctatccgcta gcgcgaaccg atcttgccag
     2461 gattatcgac gcgatcgcgc gactggagcc gaaagtcatc gcggtcgatc tcctgttggt
     2521 tgatcgagga accaacgatg gtgacgaggc gcttgaaaag tcgctcatgg gacgtgcgag
     2581 cgtgcttgcc gcggctgctg tgttcgcgca agccagccaa tcgatcgcgg cggaaaagga
     2641 cgaaccgctt tccggtctgc cgaaggccga gaagtttttg ctgccgctca agagattttc
     2701 cgatcatgcg gaaatcggca tcgtcaatgt gacgaccggt cggagcggta ctccaagctc
     2761 gattccaatg ctgttccgaa cacacgacag gatcgaaata tcgttttcgc tccgcgctgc
     2821 agcgttggcg attggtaagg agcccactat caagccggac cgtgtggtgt tcggcgagcg
     2881 atctgttgcc accgacgttg atcatacgct gccggtcact ttctatggcc cgcgccggac
     2941 catccgaacg atcagtgcag cctcggtgct tgccggtgag atcgcggcgg acgccatcca
     3001 tgatcgagtt gtcgttgtcg gggcaacggt caccggcggt ggagattttt ttccgacccc
     3061 gttcgatcca gtgatgccgg gggtggaagt cacctcaacg gcgatcacgc atcttttggc
     3121 cggggatggg atattgcgga accagtcggt gcgcctcgtt gacggtatca ttgccgtaat
     3181 gctgccgatg atcctcgtca cgctgctcgc atggcgccga agcgcgattg gccccatagc
     3241 gacgctcatt gtcgttctga cattcgcgac agcaaacgtc ttggtgtttt cgcaagggat
     3301 ctggctgagc gcggcgctgc cgatcgccgc gaccgcgcca ccggcgattt tattcgccgc
     3361 cgtgcagctc tggtcgggca ggcggcgcgc gcagtatttc gccacgcgaa gcaaactgct
     3421 cggacaattt caggcgcctg acattcagga gtggctgacc cgagatcctg gtttcctcaa
     3481 ggagccgata cgccagaatg cggcggtgat ttttatcgac ctgtccggct tcacctcgct
     3541 cagcgagagg cttggaccgg acccgatccg cgatttgctg aaggagtttc atgccctggt
     3601 cgacaaggag gcagtgagct cgggaggaat gatccccagc tttctgggtg acggcgcgat
     3661 gatcctgttt gggctgccgc aggctacgcc ggacgacgct ctcagggcag ctcaatgtgc
     3721 gacaggactc tgcgtcagcg cggagcggtg gcttgcttcg ttgccgcaac caatcaactc
     3781 gtgtcttggg ttcaaggttg gagctcattt cggtaccatc gttgcatcga gacttggcgg
     3841 cggaagttat caccacatta ccgcgactgg cgacacggtg aacgtggcga acaggctgat
     3901 ggaagtggcg gccggtcacg aagtcgaact tgccgtcagc gacgaattgc tccatgccgc
     3961 aggtcgcaat agttcactgt ttgacgtcgg tgttctcacc gggccgattg agacgcgact
     4021 tcgcggccgt tcgggctcgc ttgcggtctg gttctggcgg gtgagttcag ggacatcgtg
     4081 aacccgcgcc gccaggtctt tgtgagatag agcttggcgc ttacctcaag cggccgccgc
     4141 gttctgcttg gctgctatct tggcgcaggc tctagccgac cggcagcctg ctgtgctttg
     4201 ataggggcag gccggggcct gaaatagcga gccgtacaaa aacggtggtc ggcggcggat
     4261 gttgtcggcc ataaaagaca agggaggata ggccaatgga aaaacccggc cgtacgctgc
     4321 tctccaaaat ttgggaccag cgcgttattg cgcatatcgg tgatgacacc gatctgctcc
     4381 acgtcgatcg tcatttgctg cacgatcttg gcgggtcgcg cggcctgctc gatctcaaaa
     4441 gccgcaagct tagtgttcac aatccggaat tgaccttcgc gacgccggat cacgcgattt
     4501 cgacagcgcg cggaagggcc ggcacgagca agatagggca ggaactgctg gcgggcttga
     4561 ggaccgagac atcagccgtt ggcatcgaaa tgttcgatat cgacgagcca gggcagggca
     4621 tcgtccatgt catcggaccg gaacttggcc ttagccttcc cggttgcctg atcgtttgcg
     4681 gagacagcca tacctgcacg catggcggat tgggtgccct ggcgttcggc atcgggtcga
     4741 gcgaactaac gcatgtgctg gcaacacaag cgatcatcca gcgtcggcca aaaacaatgc
     4801 gggtgagctt cgagggtcgg ctgccattcg gcgtgacagc caaggatctg attctcgcgc
     4861 tgatcggcca catcggggct gccggaggca ccggttacgc agtcgaatat gccggcagtg
     4921 ctatccgcga cctgccgatc gaagggcggt tgacgatatg taatctttcg atcgaactcg
     4981 gtgccaagat gggtctgatc gcgccggacg agaaaacgta tgagtatctg cgtgggcgaa
     5041 cctatgcgcc gcaaggcgag atgtggcagc gcgccgtcga ggcgtggcgt cagcttccga
     5101 gcgatgctga tgctgtgttc gatcgggaag ttgtcatcga tgtcgaggcc attattccac
     5161 aggtgacctg gggcatcagc ccggaacacg tcatcggcgt cgacggtcat gttcctgatc
     5221 cccagcaaat cgcggatcca gcgcgtcgtg ccgcggtcga aaccgcgctc gactacatgg
     5281 gtctccaacc cggagcgccg atcatcggca cgcccgtcga ttgggtcttt atcggatcat
     5341 gcgccaatag ccggttgagc gatttgcgcg ccgctgccga ggtcgcgcgt ggccgcaagg
     5401 tcgcgcccgg cgtgcgcgcc tgggtcgtgc cgggttcgga gacggtcaag cgggatgctg
     5461 tggccgaagg gttagacaag cttttcaccg atgccggatt cgaatggcgc gaaccgggat
     5521 gctcgatgtg tctggccgcc aatggtgaaa cggtatctcc gggccagcgc tcggtctcga
     5581 cctccaatcg caatttcatc ggccgccagg ggccgcgcgc ccgcacccat ctggcaagcc
     5641 cggccacagc ggcggcgacg gcgatatcag gcgcaattgc cgacgtgcgg atgatggaac
     5701 actagcatgg caaaaccctt taccaaactg gcggcaatcg ccgcgccgat catgcgctcc
     5761 aacatcgata ccgacgtcat catccgcatc gatcgtctcg tcggaaattc tgttcgcggc
     5821 actctcggca agtgggcgtt cggcgcgctc cgatatctac ctgacggttc ggagaatccc
     5881 gagttcatcc tgaaccgcga gccctatcgt caggcggaaa tcctcgtcac cggtccgaat
     5941 ttcggctgcg gctcttcccg cgaaggggcg gtgtggtcgc tgcaggagat gggcatccgc
     6001 gccatcatcg gctctggctt cggcgatatt tttttcgcca actgctttca gaacggaatc
     6061 ctgcctatcg ttgtcgacaa ggagattgtc gatggtctcg ctgtggaggt tgaggccacc
     6121 cagggggcgg gacgtgtcag cgtcgatctg cgggaacaga cgatcactgc gccctcggga
     6181 aagcgtcaca gtttcgaaat cgatccgcgc cggcgcactg gactgctcga aggtctcgac
     6241 gaagtctcat tgacattgca gcgcgacgac gaaatccggg cctttcaggt gtcggatcgc
     6301 gtggagcgac cctggataca ctttgcaagg aaatcggcat gaacaaccta tccaacatga
     6361 tcaaagtggc ggtggttggc ggcgagggca tcggtcccga ggtgacggcg cagtcacacc
     6421 gaattcttaa ttggttcacc ggcagacgcg gcgttccgat gatcctgcgt gaagcgcaat
     6481 atgggctcat accttacctg gcgaccggca gagtgtcgcc agaagatacc gccgaggcga
     6541 tggacgaagc cgacgccatt gtatggggag caaccggcgg gcccgagacg aaagaggttc
     6601 ctgcggcggc tcgcaaggcg ggaagcctgc tgtcgctgcg cagcaaatac gatctttatg
     6661 ccaacctcag gcctatcgtc gccaatccgg cgctggcgga gtcggcgcct ctcaaggcaa
     6721 gtgtgctgaa gggagtcgat tttgtgatca tccgtgagct gaccagtggc atctatttcg
     6781 gagagccgcg cggcattgaa acgctcgccg atgggcaacg ccgcggcttc aacaccgagc
     6841 aatacacgac gaaccaaatt cgccgcgtgg cgcgagccgc cttcgaactg gcgcgcaccc
     6901 gaaagggaca cgtttgttcg gtggacaagg cgaatgtcct ggagaccagc gcgttatggc
     6961 gcgaggaagt tatcacgctg cacgctgcgg gattttctga tatcgagctc agccatctct
     7021 atgtcgacaa cgcggcaatg cagatcgtcc ggcagccgtc gcagttcgat gtgatggtga
     7081 ccggcaatat tttcggcgac attctttccg attgtgcggc gatggcgtcg ggttcgctcg
     7141 gcatgctgcc gtcggcctcg cttggaccgg tcgaccggtt tggtcgtcgc aaggcgcttt
     7201 atgaaccggt ccatggcagc gcgcctgaca tcgccggcaa gggcatcgcc aatccgctcg
     7261 gctccattct cagcgttgcc atgatgctgc gcatgacgct taatcggccc gacgatgccg
     7321 atctgctcga aaaggccgta gatatcgcct tggcggcggg cgcgagaacg gccgatattg
     7381 ccgaaccggg cgcaacaaaa ctatcgaccc gggaaatggg cgacgcggtg ctggacgccc
     7441 tcgaacaagt cgtcagcaag cagacggagc ccgcgtgatg acaagacatt tcacaagacg
     7501 ttcggcgctg acgatcgtcg ccggcggtgg cgcgtctgtc gcagcaggcc gagcgtgggc
     7561 cgaggacaag tccggccggg tgatctatcc ggtggccgtg ccggtctacc agacacagtt
     7621 cgtggccgac cgtatcggat acttcaagga agcgggcctc gactgcaaat tgattcaggg
     7681 cggcagcggc gtcaaaacgc gcgagatcat cgcgtcttcg caaggcgaca tcggcatcgg
     7741 cgacatcacc catccgatgc agctcagcaa tcacggccgc aacggtcggg tcctgatgcc
     7801 ggtcgacacc cgaagcagtt cggtgatgtt catcatccgc aaggatctca aagaccaggg
     7861 catcacgacg ctggaggcgt tgacggagtg gaagagaccg gacgggcgca agccgatcat
     7921 ctcggtatcc tcgttaggag gtacaaacca tgtctgggct tcctactaca tggagacgat
     7981 ggggctcgac cagaaggtca cctggatcgg aaccggcaac gtcgatacaa tgctgggttc
     8041 gctgaagacc aagcaagtcg atgttctcgt gagttcgttg tcgttgctga atgaatcggc
     8101 cgagcagggc tggggagccc tgttgttcga tggcaccgac gaagcgatct ggaacaaata
     8161 tatcggcggc aaggtcccgg taacgtctca tttcaccctg cagtcgacga tcgacaagga
     8221 cccgccgaag atgcaggcgt tcgtcaccgc tttgtggcgg gccacgcagt ggatcaagat
     8281 gcattcaccc gatgaaattt acgattctat tgagccctat gttggcagca cgtcgcgggc
     8341 cgccaacatc ttcgagatca cggcgctgca aaaagtcgcc gactatgaag ccaccatcga
     8401 tgcggcaggt tttgcgcgcg gcgagaaagt ctggtttcgg gaaatgaccg gcatcaagcc
     8461 gcttactatc gccgatgtcg tcagccccac gttcatcgaa gtggcacgaa aggcgtatcc
     8521 gggttgaaag cggtggacat gttgacgccc ccgcatcggc aggatgtaca gcgtatcgac
     8581 gtcaggggat taagcaaatc attccagctg gcgggcacgg ccatcgaggc cgtgcgcgac
     8641 gtgtccttca gcgtccaccg cggtgaattc gttgcgttgc tggggccgtc cggctccggc
     8701 aagagcacca tccttaacat gatcgcgacg ctgataaagc cgagcagcgg ccagatcctg
     8761 atcgatggtg cgccggtcat tcccggcaag gcgaccccga atgtcggcta cgtgttccag
     8821 cgcgacacgt tgtttccctg gcgcacggtc gctgacaaca tcggctacgg tctacaattg
     8881 gccggcgttc ccgaagttgt tcggaaggaa cgcatttcgg ccagcgtggc gcaagctggc
     8941 ctgcagggct tcgaacaggc ttacccgtcg gccttatcgg gcggaatgcg tcagcgcgct
     9001 gcgctgatgc gaacactggt tgtcgagccg cagatcttgc tgatggacga gccgtttggt
     9061 gcgcttgata cccacaccaa gatcgatatg cacgacgtcc tgctccggat ctgggagcgc
     9121 gagcaacaaa cggttttgtt cgtgacgcat gatctcggtg aagctttgac gctggccgac
     9181 cgtatcatcc tgttctcggc gcggccgggc atgatcaagg atacgttcga ggtggacttt
     9241 gcgcggccgc gcgacgccgt aaaggtacgg gagacgccgc gctatgccga attgttccaa
     9301 catatctggc actcgctcgg cgaagaattc gtcaagggcc gcagctcatg aggaggtcgg
     9361 gccgacgcgt gagtacatgg gcctggcagg ctctgatttg tgtcgccgtc ctttcgatct
     9421 ggcagtgggg atatgatctg catgccatcg ttccctggct ggtgcccgat ctgttcgatc
     9481 cttatttcgt gtcgaagccc tccgagatat tccagcactt tctcattctg agctgcttca
     9541 aatccaagct gggcgcggtc aatggctggt tcaacgacga ttttgccaaa tgcatggccc
     9601 gtaatgaaaa caatctatgg attgcgacgg cgatcacgct gaagaatacc ttcttcggtt
     9661 tcgtgaccgg cgtatccagc ggcttcgtcg tcgggctgat tctcggacgc tcggatcgcc
     9721 tcagtgccat tttccagccc ttcatcaccg cagttaactc cattcccagg attgctctgg
     9781 cgccgatcat cgtgctggcg ttcgggatcg g
//
LOCUS       DASWGM010000413         4150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_306222,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000413 DASWGM010000000
VERSION     DASWGM010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306222"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VGA15_12070"
     CDS             <1..1231
                     /locus_tag="VGA15_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9588462.1"
                     /translation="QKPLPREVIFVIDNSGSMGGTSIIQAKASLTYALGRLQPNDRFN
                     VIRFDHTMDVLFPHPVPADAEHVANAKSFVGALQAEGGTEMVPAMQAALTDNAAGDAN
                     TVRQVVFLTDGAIGNEQQLFDTITAMRGRSRVFMVGIGSAPNTFLMTRAAELGRGAFT
                     HIGSVEQVEERMRGLFGKLENPAVTGLAAKFSEATADLAPVAIPDLYRDEPLVLAAKL
                     DTLKGSVEIKGRIGDRPWVVTLALANAAEGKGLSKLWARRKIDDAEIARTLRTATPEE
                     ADKTILALALEHQLVSRLTSLVAVDKTPSRPEGEALKLSELPLNLPAGWDFAKVFGER
                     PKLPSPPAERRADAGDARVHLAAAKHMPLAVMPAPGTVKLQKTATDAELKMIAGSILL
                     ALSLILFVFNRRQISPS"
     gene            1463..2065
                     /locus_tag="VGA15_12075"
     CDS             1463..2065
                     /locus_tag="VGA15_12075"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HEX9588463.1"
                     /translation="MTRFTVPLLLALAGLVLFGQGAYIHAKALLAQVLLERAFAETIA
                     SGRATKPWSWADTWPVARIEVRRLGASAIMLAGSSGQALAFGPGHVELTPNAGERGVA
                     VYSAHRDTHFRFLKKVAIGDEIDITRNDGRTFRYRADGAAVVRFDASGIDPLTNGYEL
                     VLSTCWPFDAVAPGPTRYVLHATMIGLAGTSDEQVPNGSN"
     gene            2245..2964
                     /locus_tag="VGA15_12080"
     CDS             2245..2964
                     /locus_tag="VGA15_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HEX9588464.1"
                     /translation="MSSQVHYLPLTPGLFSILVLLFVVLLILIQLRILRYAYMRLGIG
                     PGMALLLLFGSLIGSYFNIPITVLAGPAVKSGQIVEFFGMRYIAPVVVAWPGTVLAIN
                     VGGAVIPTVMSAYLMFRYQFWLKATLVTAAIALVIHSMATPVPGIGIAVPVFAPVMTT
                     AILAFLLSREYAPPLAYIGGSMGTLIGADLMNLDKITGLGAPVASIGGAGTFDGIFLT
                     GILAVLLAGVASPSRPPRPAW"
     gene            3117..>4150
                     /locus_tag="VGA15_12085"
     CDS             3117..>4150
                     /locus_tag="VGA15_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9588465.1"
                     /translation="MEAQVSVASDSAMKWSPSSDASDIVKRIHAMLHPRNIVLVGATD
                     KPGNYAERIWNNLIKYRYEGGLYPVNAKRETIWGVPCYKDFASLPEEPDHVLVLVPAR
                     FAVQVVRDAAAAGARSATIVTSGFSELQDDESQRLAAELQAAIRETGLAVTGPNCLGN
                     LSAGEKLFTNIDDRVVTMEAGPVAIAGQSGAIVMAIRQALEDRGVGVGYMVTTGNEAG
                     LETPDLMAYFAADPSIRVIVVYLEGVRNTQVFRRACKAARAAGKPVIALKLGASEGGR
                     AAAMAHTGALAGSIETFDAISTREGVIRVRGLDELIETTECFVHADPPNGSRIAAVSL
                     SGGKRGLLIDA"
BASE COUNT          737 a         1347 c         1275 g          791 t
ORIGIN      
        1 acaaaagccg ctgccgcgcg aagtgatttt cgtgatcgac aattccggtt cgatgggcgg
       61 cacctcgatt atccaggcca aggcgagcct cacttacgcg ctcggccggc tgcagccgaa
      121 cgaccgcttc aatgtgatcc gtttcgatca caccatggac gtgctgttcc ctcatccggt
      181 gcccgccgat gcggagcatg tcgctaacgc caaatcgttc gtcggtgcat tgcaggccga
      241 aggcggcacc gagatggtgc cggcgatgca ggcggcgctg accgataacg ccgccggcga
      301 cgccaacacc gttcgccagg tggtgttcct gaccgatggc gcgatcggca acgagcagca
      361 attgttcgac accattaccg cgatgcgcgg ccgttcccgc gtgttcatgg tcggcatcgg
      421 ttcggcgccg aacacgttcc tgatgacccg cgccgctgaa ctcggccgcg gagcctttac
      481 ccatatcggc tcggtcgagc aggtcgagga gcggatgcgc ggcctgttcg gcaagctcga
      541 aaatcccgcg gtgacgggcc ttgccgcgaa gttctccgag gccacggccg acctcgcgcc
      601 ggtggcgatc cccgacctct accgcgacga gccgctggtt ctcgcggcga agctcgacac
      661 gctcaagggc tcggtcgaga tcaagggccg gatcggcgac cggccctggg tcgtgacgct
      721 tgccttggcg aacgccgccg aaggcaaggg tctctccaag ctgtgggcgc ggcgcaagat
      781 cgacgacgcc gagatcgccc gcaccctgcg aacggcgacg cctgaggaag ccgacaagac
      841 catcctggcg ctggcgctgg agcatcagct cgtaagccgg ttgaccagtc tggtcgcggt
      901 cgacaagacc ccaagccgtc ccgaagggga agcgctcaaa ctcagcgaat tgccgctcaa
      961 cctgccggca ggctgggatt ttgcgaaggt gtttggcgag cgcccaaaac ttccgtcacc
     1021 cccggccgag cgtcgcgccg acgccggcga cgcccgggtt catctcgcgg cggcgaagca
     1081 catgcccctc gccgtcatgc cggcgcccgg caccgtcaag ttgcagaaga cggcaaccga
     1141 tgccgaactc aaaatgatcg ccgggtcgat cctgctggcg ctcagcctga tcctgttcgt
     1201 gttcaaccga cgtcagatat ccccaagttg acgccggatg aaaggaggag ttgtcccccc
     1261 ctcctcctca aaggcgcgcg cggcctccca cccaccaggg ccgcgcgcgc agctttcctc
     1321 caccgtcatt ccgggatggt ccgaaggacc aggcccggaa tctcgagatt ccgggttcga
     1381 tgcggagcct gtcatcgggc tgggctttgc gcggacccgt tggcatcgcc ccggaatgac
     1441 agaaacaata ttgagattcc tgatgacccg cttcaccgta ccgctgcttc tcgcgctcgc
     1501 cggcctcgtg ctgttcggtc aaggcgctta cattcatgcc aaggctctat tggcgcaggt
     1561 tctgctggag cgcgcctttg ccgagaccat cgccagcggg cgcgcgacaa aaccctggtc
     1621 atgggcggat acctggccgg tcgcacgcat cgaggtgagg cggctcggtg ccagcgctat
     1681 catgctggcc ggaagcagcg gccaagcgct tgccttcggg ccgggtcacg tcgagctcac
     1741 gcccaatgcg ggcgaacgcg gcgtggcggt ctattcggcg caccgcgata cgcatttccg
     1801 ctttcttaag aaagtcgcaa ttggcgacga gatcgatatc acgcgaaacg acggccgcac
     1861 cttccgctat cgtgcggacg gtgctgcggt ggtgcgtttc gatgcctcgg gcatcgatcc
     1921 cctaaccaac ggatacgaat tggtgctgtc gacctgctgg ccattcgatg cggtggcgcc
     1981 agggccgacg cgctacgttt tgcacgccac catgatcggg ctagccggca cttccgatga
     2041 acaggtgccg aatggttcaa attgagttcg gtgctggcca ggacgcgggc cgaggctttt
     2101 tgccgcatgc cagccggcgg cgtcatgctg gttcatggcc aatcgcatat ccgcgcttga
     2161 atgttgttcg gctacactct tgccgtgcgg cggaaaatca ataattgaaa catgacaaac
     2221 aaccgggatt gaaccagcga gatcatgagt tcccaagttc actatttgcc gctgacaccg
     2281 ggattgtttt cgatcctggt cttgctgttc gtggtactgc tcatcctgat tcagcttcgc
     2341 attctcagat atgcctatat gcggctcggg atcggtcccg gtatggccct acttctgctg
     2401 ttcggctcgc tgattggcag ctatttcaat attccgatca cggtgctggc cggacccgcc
     2461 gtcaaatcgg gacagatcgt tgaattcttc ggcatgcgtt acatcgcgcc ggtcgtggtg
     2521 gcgtggcccg gcacggttct cgccatcaac gtcggcggcg ccgttattcc aacggtaatg
     2581 tccgcgtatc tgatgtttcg gtatcaattc tggctcaagg caacgcttgt caccgcggcc
     2641 atcgccttgg tgatccattc gatggcgacg ccggtgccgg gaattggtat cgccgtgccg
     2701 gtgtttgcgc cggtcatgac cacggcgatc cttgcatttc tcctgtcccg cgaatacgcg
     2761 ccgccgctgg cctatatcgg cggctcgatg ggaacgctga ttggcgccga tctcatgaat
     2821 ctcgacaaga tcaccggcct cggcgctccg gtcgcctcga ttggtggcgc cgggacattc
     2881 gacggcatct tcctgaccgg cattcttgcc gttctgctcg ccggtgtcgc ctcgccgtcg
     2941 cgcccaccac gaccggcgtg gtaagtccct gtacgggtgg ccgcgggcat cggcccgttc
     3001 cgcgagttgc caccctcccc gactatcaat aataagaaat caaaaggtca cggggaacgt
     3061 cgatctccag tcgatcgcca ttttccgcat aaaaaaccaa gggtaaggtt acgcctatgg
     3121 aagctcaggt aagcgtcgcg tccgattcgg ccatgaaatg gtcgccttcc tctgacgcaa
     3181 gcgacatcgt caaacgcatt catgcgatgc tgcatccgcg caacattgtg ctggtcggag
     3241 cgacggacaa gccgggcaac tacgccgaac ggatctggaa caatctgatc aagtatcggt
     3301 acgaaggcgg tctctacccg gtcaatgcca agcgcgaaac catctggggc gttccctgct
     3361 acaaggattt cgccagcctg ccggaagagc ccgatcacgt gctggtgctg gtgccggcgc
     3421 gtttcgccgt gcaggtcgtg cgcgacgccg cggcggcggg cgcccgctcc gcgaccatcg
     3481 tcacctccgg cttcagtgaa ttgcaggacg atgaaagcca gaggctcgcc gccgaactgc
     3541 aggcggcgat ccgcgagacc gggcttgcgg tcaccggccc gaattgtctt ggcaatctga
     3601 gcgccggcga aaagctgttc accaacatcg acgaccgcgt cgtcaccatg gaggccggac
     3661 cggtcgcgat cgccggtcaa tccggcgcga tcgtcatggc gatccgccag gccctggagg
     3721 atcgcggcgt cggcgtcggc tacatggtca ccaccggaaa cgaggccggc ctcgaaaccc
     3781 cggacctgat ggcctatttc gcggccgacc cctcgatccg cgtgatcgtg gtttatctcg
     3841 aaggcgtgcg caatacccag gtctttcgca gggcatgcaa ggccgcgcgc gcggccggca
     3901 agccggtgat cgcgctcaag ctcggtgcgt ccgaaggcgg acgcgccgcc gcgatggcgc
     3961 ataccggcgc actcgccggc tcgatcgaga ccttcgacgc catctcgacc cgggaaggcg
     4021 tgatccgcgt gcgcggtctc gacgaattga tcgaaaccac cgaatgcttt gtccatgccg
     4081 atccaccgaa cggttcccgc atcgccgcgg tcagcctgtc cggcggcaag cgcggtctcc
     4141 tgatcgacgc
//
LOCUS       DASWGM010000414         3698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_873665,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000414 DASWGM010000000
VERSION     DASWGM010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873665"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VGA15_12090"
     CDS             complement(<1..1074)
                     /locus_tag="VGA15_12090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9588466.1"
                     /translation="MNKMKAGSATVDSRTAWIVAGASLVILTIAYGAPLLSAVALRPI
                     AAEFGTERAAPAAAPAFTLIGAAFGGIAAGWLSGWLGVRRIVLFGAAMIAAGLVLSAS
                     GGLIHLYVGHGLLMGLFGTSCIFSPVVTYVSRWFDRSRGAAVAMISSGQSLSGALWPI
                     LFQTGITEFGWRRTMLVFGLFVGASILALAAIFLRPPPEARPSLGGGGRDPQAGAPVF
                     GLSPNLAMIVLMIAIFCCCVPMAMPSQHIVAFCGDLGFSSRSGAAMLSVLLGSAFLAR
                     QFWGWLADRIGGLQTLLWSSLAQATALTGFLLTVDEAALFAVSAAFGLGFSGLLPAYV
                     IVIRKFYPVKEANWRVPTIYFAGF"
     gene            complement(1539..1964)
                     /locus_tag="VGA15_12095"
     CDS             complement(1539..1964)
                     /locus_tag="VGA15_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588467.1"
                     /translation="MSATQARADFARYLKSTDFAKAVVHLPNDTHVLFERVFGARHFS
                     WRCVKTSVLFSVAAFAIIVVLNVLNHPKGSLSRTLNVLEMFPGFFRSVVAYAIWSIVP
                     DYFNLLKTRKVLGLITARQMGQVDWNHVVSNFGFDPLPN"
     gene            1983..2096
                     /locus_tag="VGA15_12100"
                     /pseudo
     CDS             1983..2096
                     /locus_tag="VGA15_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021095.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            complement(2373..3431)
                     /locus_tag="VGA15_12105"
     CDS             complement(2373..3431)
                     /locus_tag="VGA15_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9588468.1"
                     /translation="MAEGVRKDEEFSGTKPVEERHRIDEAHLDGWMRDHVEGYAGPLT
                     VLQFKGGQSNPTYRLDTPARSYVMRRRPFGKLLPSAHAVDREFRVIAALSKQGFPVAK
                     AYALCTDDAVIGAFFYIMSMEEGRVFWDPTLPSQTPEARRKIFTSKIETLARLHTYDP
                     QAIGLGDFGKPGNYFARQVDRWTKQYRASETQHIPEFEKLAEWLPKTVPPQQRVSIVH
                     GDYRLDNMIFHAREPRVQAVLDWELSTLGDPMADFTYLLMQWTMPGLANADLAALNIP
                     SMEEAAQIYCNVTGSAVPDLNWYFSYNLFRLAGITQGIAGRIRDGTAANAKAIESAKR
                     TVPLSQASWEYAQKAGAK"
     gene            complement(3482..>3698)
                     /locus_tag="VGA15_12110"
     CDS             complement(3482..>3698)
                     /locus_tag="VGA15_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9588469.1"
                     /translation="MIKVAAPNMALKIIDNAIQAYGGAGVSDEAGLARDYASMRTMRL
                     ADGPDEVHNRAIARLELRKYANSPTTH"
BASE COUNT          760 a         1127 c         1154 g          657 t
ORIGIN      
        1 gaaacccgcg aaatatatcg tcggcacccg ccagttcgcc tccttcaccg gatagaattt
       61 gcggatcacg atcacgtagg ccggcaacag tccggagaag cccagcccga aggccgcgga
      121 aaccgcgaac aaggcggcct cgtccacggt cagcaggaag ccggtcaacg ccgtggcctg
      181 ggccagagaa ctccagagca gcgtctgcaa gccgccgatc cggtcggcaa gccagcccca
      241 gaactggcgc gccaggaacg cgctgccgag cagcaccgac agcatggcag cgccgctccg
      301 cgatgagaag ccgaggtcgc cgcagaacgc cacgatatgc tgcgacggca tggccatggg
      361 cacgcagcag cagaagatcg cgatcatcag gacgatcatg gccaggttcg gcgaaagtcc
      421 aaagacgggc gcgcccgctt gcggatcgcg tccgccgccg cctagagagg gccgcgcctc
      481 gggcggaggg cgcaggaaga tcgcggccag cgccaggatc gaggcgccga cgaacagccc
      541 gaacaccagc atggtccggc gccagccgaa ctcggtgatg ccggtctgga acaggatcgg
      601 ccacaaagct cccgacagcg attgacccga cgagatcatg gccacggcag cgccccggct
      661 gcgatcgaac cagcggctga cataggtcac gaccggcgag aatatgcagg aggtgccgaa
      721 caggcccatc agcaggccgt gtcccacata gaggtggatg agtccgccgg aggcggagag
      781 gacgaggccg gcggcgatca ttgccgcacc gaacagcacg atccggcgga cgccgagcca
      841 gccggacagc cagcccgccg cgatgccgcc gaaggccgca ccgattaagg tgaacgctgg
      901 ggcggcggcg ggcgcggcgc gctcggtgcc gaactcggcg gcaattggcc tgagcgccac
      961 cgcggagagc aacggcgcgc cgtaggcgat cgtcaggatg acgagggacg cgccggcaac
     1021 gatccaggcc gtgcgactgt ccaccgtcgc ggaaccggct ttcatcttgt tcattgtcgg
     1081 atcccgtgca ccgcgtattt gccggcggtg acgagtcgga ggactgccgc aagcctgcaa
     1141 atcgagatta ggccatcctt gcctggcctt tatccaattt gcgacaggcc cgtcctcggc
     1201 gcgtcgtttt caccgcgatc gggtatcaat acgtagaaat tgaaaagcaa catcagaccg
     1261 tcatcgcttg ttttgctgct cgcggttaca aaggttggga cgtgcacctg caatcgttcg
     1321 ctcggcaccg agataaatgc gtgcgcgtta tgtcgcgctc agctgtagaa gcaaacgcac
     1381 tctaaccaac aggtctcaac tcatcggtca gttccgccga cagcacggag aaattgttaa
     1441 cgaaattgag cgggttcttg atcaacagcg gcgaagtccg aaacgggtcc aggctgtgtg
     1501 aaaagtcgca cgagccaagg gcgcgcagaa ttgttttctc aattggggag cgggtcgaaa
     1561 ccgaaattac tgacgacgtg attccaatct acctgcccca tctggcgcgc cgtgatcagg
     1621 ccaagtacct tgcgcgtttt gagcagattg aaataatccg gcacgattga ccagattgca
     1681 tacgccacaa ccgaacggaa gaaaccggga aacatttcca acacgttgag cgtccgagag
     1741 agcgatccct tcggatggtt cagaacattc aataccacta tgatcgcgaa agcagcgaca
     1801 gagaaaagaa ccgaagtttt tacgcatcgc caggaaaaat ggcgtgctcc gaacacgcgc
     1861 tcaaacagta catgtgtatc attaggtaag tgcacgacgg ctttagcgaa gtcggtcgat
     1921 ttgaggtatc gtgcgaaatc ggctcgcgcc tgtgtcgcgc tcatgttggt ggcgccgcgt
     1981 gggcggcgat gggattgttt ctcgccagag acgacgcgtc ctatgtcaat ggacaggcga
     2041 tcccggtcga cggcggcctc accgcctcga tgccgcatgc aggcaagccg atttgatccg
     2101 aagaagccct gccttaacct cgccccgctc tttgcgggag aggtcgaagc ctgagcgcag
     2161 cgatggcttc gggtgagggg gcgaggcagt gcgcacaata cgattgcacg actctcgggt
     2221 cgcgttaggg ctcgcggcat cgttcgtagt caaggatccg acccgcggat gcagagggcg
     2281 gagtgagcgc agtgcctggc ccctcacccg ctcgcttcgc tcgcgacctc tccccgcaaa
     2341 gagcggggcg aggtttagat cacgccgtcg tgtcacttcg cccccgcctt ctgcgcgtat
     2401 tcccaggaag cctgcgacag cggcacggtg cgcttcgccg actcgatggc cttggcgttg
     2461 gccgcggtgc cgtcgcgaat gcgtccggcg ataccctggg taatgccggc aaggcggaac
     2521 aggttgtagg aaaaatacca gttcaggtcg ggaacggcgc tgccggtcac gttgcaatag
     2581 atttgcgccg cctcttccat gctcggaatg ttgagcgccg caaggtcggc attggcaagg
     2641 ccgggcatgg tccattgcat caatagatag gtgaaatccg ccatcggatc gccaagcgtc
     2701 gacagctccc agtccagcac cgcctgcacc cgcggttccc tagcatggaa gatcatattg
     2761 tcgaggcgat agtcgccgtg cacgatcgaa actcgttgct gcggcggcac ggttttcggc
     2821 aaccactcgg ccagtttttc gaattcggga atgtgctggg tttcggacgc gcgatattgc
     2881 ttggtccagc gatcgacctg ccgtgcaaaa taattgccgg gtttaccgaa atcgccgagc
     2941 ccgattgctt gcggatcgta ggtgtgcagc ctggcgagcg tctcgatctt gctggtgaag
     3001 atcttgcgcc gcgcctccgg cgtctggctc ggaagcgtgg gatcccagaa cacccgcccc
     3061 tcttccatcg acatgatgta gaagaaagcc ccgatcacgg catcgtcggt gcacagcgca
     3121 taggctttgg cgaccggaaa gccctgcttg ctcaatgctg cgatgacacg aaattcgcga
     3181 tcgaccgcat gcgccgacgg cagtaattta ccgaacggcc tgcggcgcat cacgtaggat
     3241 cgcgcgggcg tgtcgagccg gtaggtcgga ttggactggc cgcccttgaa ctgcagaacg
     3301 gtcagcggtc ccgcatagcc ttcgacatgg tcgcgcatcc agccgtcgag atgcgcctcg
     3361 tcgatccgat gccgctcctc gacgggcttg gtgcccgaaa actcctcgtc ttttctgacg
     3421 ccttcggcca cgatgacgct ccctcaagtt tttttaagga gcgccatgtg tgcggctccc
     3481 gttagtgcgt agtgggagag tttgcatact tccgaagttc aagtctggca atggcgcggt
     3541 tatggacctc gtccggcccg tccgccaggc gcatcgtgcg catcgaggca tagtcccgcg
     3601 ccagaccggc ctcgtcggag acgccggccc caccgtacgc ctggatggcg ttgtcgatga
     3661 tcttcagcgc catattcggc gccgccacct tgatcatg
//
LOCUS       DASWGM010000415         7033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1144579,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000415 DASWGM010000000
VERSION     DASWGM010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144579"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VGA15_12115"
     CDS             complement(<1..612)
                     /locus_tag="VGA15_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HEX9588470.1"
                     /translation="MATKAKTLQVKDKEKDDKAADAPEKDSPDAPSPLLDLSDAAVKK
                     MIKQAKKRGFVTFDQLNEVLPSDTTSPEQIEDIMSMLSDMGINVSEAEDSDSDEETKE
                     EAEDETDNELVEVTQKAVTEVKKSEPGERTDDPVRMYLREMGTVELLSREGEIAIAKR
                     IEAGREAMIAGLCESPLTFQAIIIWRDELNEGKIFLRDIIDLEA"
     gene            complement(963..2996)
                     /gene="dnaG"
                     /locus_tag="VGA15_12120"
     CDS             complement(963..2996)
                     /gene="dnaG"
                     /locus_tag="VGA15_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX9588471.1"
                     /translation="MRFTPQFLDELRARLPVSEVVGRRVKLKRAGREWKGLSPFQQEK
                     SPSFTVNDQKGFYHDFSSGKHGDIISFLMETEGVGFTEAVERLASMAGVPLPAATPDA
                     ARHEQRRKTLYDVMDLAAKFFSSTLASRNGAKARGYLGDRAISPAVQLQFGIGYAPGE
                     RFALKEHLGALGIPVEDMVEAGLLIAGDDIPVPYDRFRDRVMFPISDLRGRVIAFGGR
                     ALEKDVAAKYLNSPETPLFHKGDNLYNLAPARLTAHNGSALVVVEGYVDVIAMVGAGF
                     PASVAPLGTALTENQLALLWKMADEPILCFDGDKAGQKAAWRAADMALPHLLPGKSLR
                     FALLPEGQDPDDLARSGGRGAVEEVISAARGLADVIWSREIEGGTFATPERRAALEKR
                     INELTNGIRDEVVRRYYRQDLAERLQRTFAPEGGRGGYGRGNFRGGAGNFGRESGRAF
                     SPRGSFTPGAAGRSEPSRGGRPQGSAGSQTINRGPYQVASPQLALSPIMRGQRSAISR
                     REALILQSLINHPWLLHDHLEEVAALELAHPEAHRLRAGIIAAFAHDHHHSPEPSEQS
                     EKMRADLAAGGFLQSLQRVELAITTSAVWGAKGSAAREDVLSTWHQLVSLHRQWHSLL
                     RELKDAELALGDEASEANLAWLRDVKARLAEVDGTEALIEGFGESSGRFQRSV"
     gene            3165..3488
                     /locus_tag="VGA15_12125"
     CDS             3165..3488
                     /locus_tag="VGA15_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX9588472.1"
                     /translation="MADLEFEFLAPAGFEWDEDKSEVNLIKHGIDFDDASEVFYRPVV
                     VWRSNRNNEERWIAIGKSHDQIVSVIFTHRGDLIRIISARRPRSNEKRAYRDASMGRS
                     AQGKD"
     gene            3508..3747
                     /locus_tag="VGA15_12130"
     CDS             3508..3747
                     /locus_tag="VGA15_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX9588473.1"
                     /translation="MTDAEVEASIANDPDWAEFKDIDWSDAVLVIPTRKKAISIRVDE
                     DVLDYFKSEGDGYQRRINAVLRSYMQQKNKPKKRA"
     gene            complement(3960..4355)
                     /locus_tag="VGA15_12135"
     CDS             complement(3960..4355)
                     /locus_tag="VGA15_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX9588474.1"
                     /translation="MMLDRLFVYGTLMRGFDHPMAQLLSRGADLIGEARCRGRLYLVT
                     HYPALVLSGDPADVVFGELYRLRAPDELLREFDMYEACGEGFAAPTEYVRQMLSVTLE
                     GGATEAWTYVYNWPVAHLARIASGRFLAR"
     gene            complement(4378..5190)
                     /locus_tag="VGA15_12140"
     CDS             complement(4378..5190)
                     /locus_tag="VGA15_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9588475.1"
                     /translation="MEIKVGFEIAHTAVQPTPMVIMLSIHPSRYSDIIGTESIVAEPN
                     VPIGFYRDSFGNVCGRLVAPAGGVTLRGSALVRDSGLPDVVVPTAQQLPIDQLPDDLL
                     FYLMASRYCETDKLTDIAWSLFGNTQPGWARVAAICTYVHNHVTFGYEHANYMKSAHD
                     VYEQGAGVCRDFAHLALTFCRCMGIPARYCTGYMGDIGIPPDPAPMDFSGWFEVYLSG
                     QWYTCDARHNHPRIGRILMGTGRDAADVALTTSFGRMDLVKFVVISDEVVAA"
     gene            complement(5192..6082)
                     /locus_tag="VGA15_12145"
     CDS             complement(5192..6082)
                     /locus_tag="VGA15_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9588476.1"
                     /translation="MPLLTIHHKTEYRYAHPVAFGEHRIMLRPRDGHDLRVLSGSLEI
                     VPEPMSLRWIHDVFGNSVAIATFDERAETLTFSSTATVEHNPAEEYVPTPDDPAYFYP
                     FFYNDEEFPDLIQFITPQYGDPDGELSAWARNFLDEEGPTPTFNILSDMTHGIRKAFT
                     YRKRHEHGTQHPLDTLQTRSGTCRDYALFMIEALRRLGIAARFVSGYLFIPGDRSHGY
                     VGGGSTHAWVQAYLPSAGWIEFDPTNGIVGTKDLVRVAVARDPRQAVPLHGTYLGSAD
                     AYVGMEVSINVVSVDMGAEA"
     gene            6183..6986
                     /locus_tag="VGA15_12150"
     CDS             6183..6986
                     /locus_tag="VGA15_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX9588477.1"
                     /translation="MTLDNAGDTSLLLGSTDVPPVLEDNAAGGSPFLLTCDHYGRLTP
                     RSLGDLGLPASELTRHIAWDIGIAGVAEALSKQLDAHLIAQRYSRLVIDCNRPPGAPS
                     SIPRISEATTIPANEALSRDAAEVRRQAIFDPYHRRIAEVIEARLREGVPTVLVSLHS
                     FTPVYAGIARPWHIGTLYQRDIRLPQLLLKQLRGEGDLVVGDNEPYAVSDETDYTIPV
                     HGEARGLMNTGIEIRQDLIADQAGEQQWADRLARIFGEIETALRAQRLV"
BASE COUNT         1352 a         2230 c         2164 g         1287 t
ORIGIN      
        1 cgcttccaga tcgatgatgt cgcgaaggaa gatctttccc tcgttgagtt cgtcgcgcca
       61 gatgatgatg gcctggaagg tcagcgggct ttcgcacagc cctgcgatca tcgcctcgcg
      121 gccggcctcg atgcgcttgg caatggcgat ttcgccctcg cgcgacaaca gctcgacggt
      181 gcccatctcg cgcagataca tgcggacggg atcgtcggtg cgctcgccgg gctcggattt
      241 cttgacttcg gtgacggcct tctgggtgac ctcgaccagt tcgttgtcgg tctcgtcctc
      301 ggcctcttcc ttggtttcct cgtcgctgtc ggaatcctcg gcctcggaga cgttgatgcc
      361 catgtccgag agcatcgaca tgatgtcttc gatctgttcc ggcgaggtgg tgtcggacgg
      421 caagacttcg ttgagctgat cgaaggtcac gaagccgcgc ttcttggcct gcttgatcat
      481 cttcttgacg gcggcatccg acaggtcgag caacggcgac ggcgcgtccg gagaatcctt
      541 ctcaggggcg tccgctgcct tgtcgtcttt ttccttgtct tttacctgca gcgtctttgc
      601 cttggtggcc attcattgct cccgaacgcg ctcatgcagg aagcgtcacc gcgcccgcct
      661 tgaatactga accgacttat gacgcggaaa gggcggcgca tacttgtcgc caccccctgg
      721 tctgtgtcac tggatttcgc acaatggcct gatgcatatt gcgggaaatg tggacaccgc
      781 ctatccgaac agaacatgcg ccaagtcatt cctgacactt ttctgacagc aaagcggcgg
      841 ccacatgacc ccgaaaatac accaaagcca ttacgcttcg attaaccctg tttttgccgc
      901 caaaccatgg gtttggcgag tcatttcccg gctccggcca ggccgtccgc atgaattttc
      961 aatcaaacgc ttcgctggaa ccggcccgac gactcgccga aaccctcgat caatgcctcg
     1021 gtgccgtcca cttccgccag ccgggccttg acgtcccgca gccacgccaa attcgcctcg
     1081 cttgcctcgt cgcccaaggc cagctcggcg tctttcaatt ccctaagtaa tgagtgccat
     1141 tggcgatgca aggagacgag ctggtgccag gtggaaagaa cgtcctcccg cgccgcggag
     1201 cctttcgccc cccacaccgc cgaggtcgtg atcgccagct caaccctttg aagtgattgc
     1261 aaaaatccgc ccgcggcgag gtcggcccgc atcttctcgc tctgctctga gggctccggg
     1321 ctgtggtggt ggtcatgggc gaaggcagcg atgatgccgg cccgcagtct gtgcgcctcc
     1381 gggtgggcca attcgagcgc ggccacctcc tccagatggt cgtgcagcag ccagggatgg
     1441 ttgatcaggg actgcaggat cagcgcctcg cggcgggaga tcgcgctgcg ctggccgcgc
     1501 atgatcgggc tcaatgccag ctgcgggctc gccacctgat agggcccgcg gttgatggtc
     1561 tggctgcccg cagatccctg tggacggccg ccgcgcgagg gctcggaccg gcccgccgcc
     1621 cccggggtga acgatccacg cggcgaaaaa gcccggcctg attcgcggcc aaaattgccg
     1681 gcccccccgc gaaaattgcc ccggccgtag ccgccgcgcc cgccctccgg cgcgaacgtc
     1741 cgctgcaacc gctcggccag atcctggcga taataacgcc gcaccacctc gtcgcggatg
     1801 ccattggtca gctcattgat ccgcttctcc agcgccgcgc gccgctcggg cgtcgcaaac
     1861 gtgccgcctt cgatttcgcg cgaccagatc acatcggcga ggccccgcgc cgccgagatc
     1921 acttcctcga ccgccccgcg tccgccggag cgcgcgagat cgtcggggtc ctgtccttcc
     1981 ggcagcagcg caaaccgcag gctcttgccg ggcaacagat gcggcagcgc catgtcggca
     2041 gcgcgccacg ccgccttctg tccggccttg tcgccgtcga aacaaagaat cggctcgtcc
     2101 gccatcttcc acaacagcgc gagctggttt tcggtcagcg cggtgccgag cggggcaacg
     2161 ctcgccggaa aaccggcgcc gaccatggcg atgacgtcga catagccttc caccaccacc
     2221 agggcgcttc cgttatgggc ggtcagccgc gcgggcgcta aattgtaaag attgtcgccc
     2281 ttgtgaaaca gcggggtttc cggcgagttc aggtattttg cggcaacatc tttttccagc
     2341 gcgcggccgc cgaacgcgat cacccgtccg cgcaggtcgc taatcggaaa catcacgcgg
     2401 tcgcgaaacc ggtcatacgg caccgggatg tcatcgcccg cgatcagcag gccggcttcc
     2461 accatgtcct cgaccggaat cccgagggcg ccgaggtgct ccttcaaggc aaaccgttcg
     2521 ccgggcgcat agccgatgcc aaactgcaat tgcaccgccg gcgaaatcgc gcggtcgccg
     2581 agatagccgc gcgccttggc gccgttgcgc gaggccaacg tgctggaaaa gaacttcgcg
     2641 gcaagatcca tcacgtcata cagcgtcttg cggcgctgct cgtgccgcgc cgcatcgggg
     2701 gtggcggcgg gcagcggcac cccggccatc gatgccaagc gctcgacggc ctcggtaaaa
     2761 ccgacgccct cggtctccat caggaaggag atgatgtcgc cgtgcttgcc ggacgaaaaa
     2821 tcgtgataaa atcccttctg gtcattgacc gtgaacgacg gcgacttttc ttgttgaaat
     2881 ggcgacagtc ccttccattc gcgccccgcc cttttcaact tcacacgccg gcccacgact
     2941 tccgaaaccg gaagccgggc gcgcagctcg tcgaggaatt gaggcgtaaa gcgcataagg
     3001 gattttgtgt gagtcggggg cggctgccaa ccgatcatgg atataggggc ggttggctaa
     3061 aatacgagaa ttgttggctt tctcccggct attcacagga tcaggctgtg acgtcaggat
     3121 tgacaaatga aatacaattg tatatacaag ccaaaaggct caaaatggct gatcttgaat
     3181 tcgaatttct cgcgccggcg ggcttcgaat gggatgagga caagagcgaa gttaacttaa
     3241 tcaaacacgg catcgacttc gacgacgcaa gcgaagtttt ttacagaccg gttgtcgtct
     3301 ggcggtcaaa tcgtaacaac gaggagcgat ggatcgcgat cggaaaatct catgatcaaa
     3361 tcgtatcggt gatttttact cacagaggcg acctgatccg aattatttcg gcgcgccgtc
     3421 cgagatcaaa tgaaaaaaga gcgtatcgtg acgcgtcgat ggggcgatcg gcgcaaggga
     3481 aagactgact gggcgcgttt cgacgcaatg acggacgcgg aagtcgaagc ctcgatcgcg
     3541 aatgatcctg attgggcgga gttcaaggat atcgactggt cggacgccgt tctggtcata
     3601 cccaccagga aaaaggcgat ctcgattcgt gtcgacgaag acgtgctcga ctatttcaag
     3661 agcgaaggcg atggctatca gcgccgcatc aatgccgtgc tgcgttccta catgcagcag
     3721 aaaaacaaac cgaaaaagcg tgcctgacgc cgcaattcac taccgctcct gaattcgccc
     3781 cggcgatccg cgactgagcc gaccgaacac aggcatcgtg ccccggatgc tgcgcggcgc
     3841 gccgcaatag cggcgtggtg cgctgctggt ccggggccca tcgctacacg gcggaagcgt
     3901 gggtcccggc tctgcggagc agcgtgaagt acgccgcacc gcgtccggga cacggggctt
     3961 taccgcgcca gaaaccgtcc cgaggcgatg cgcgcgaggt gcgcgacggg ccagttgtag
     4021 acgtaggtcc aggcctccgt cgcaccaccc tcgagcgtca cggatagcat ctgcctgaca
     4081 tattcggtcg gcgcggcaaa accttcgccg caggcctcgt acatgtcgaa ctcgcgcagc
     4141 aattcgtcgg gcgcacgcag ccgatagagt tcgccgaaca cgacgtccgc ggggtcgccg
     4201 gacagcacca gcgcgggata atgcgtgacc agataaagcc ggccccggca ccgcgcctcg
     4261 ccgatcaaat ccgcgccgcg cgacaagagc tgcgccatcg gatggtcgaa gccgcgcatc
     4321 agcgtgccgt agacgaacag gcgatccagc atcatgcccc acgaacggcg aaaagcctta
     4381 agccgccacg acctcgtcgc tgatgacgac gaacttcacc agatccatcc gcccaaagct
     4441 cgtggtcagc gccacgtcgg cggcgtcgcg gccggtgccc atcaggatgc ggccgatccg
     4501 cggatgattg tggcgggcgt cgcaggtgta ccactggccg gacagataga cctcgaacca
     4561 gccggaaaaa tccatcggtg cgggatccgg cggaattccg atgtcgccca tatagccggt
     4621 gcaatagcgc gccgggatgc ccatgcagcg gcagaaggtc agcgccagat gcgcgaaatc
     4681 gcgacagacg ccggcgccct gctcatagac gtcatgcgcg gatttcatgt agttcgcatg
     4741 ctcgtagccg aaggtgacgt ggttgtgcac gtaggtgcag atcgcggcga cccgcgccca
     4801 gcccggctgg gtgttgccga acagcgacca cgcgatatcg gtcagcttgt cggtttcgca
     4861 atagcggctc gccatcagat agaacagcag gtcgtcgggc agctgatcga tcggaagctg
     4921 ctgcgcggtc ggcaccacga cgtcgggcaa tccggaatcg cgcaccagcg cgctgccgcg
     4981 caaggtcacg ccgccggccg gcgccaccag cctgccgcaa acgttgccga agctgtcgcg
     5041 gtaaaacccg atcggcacat tgggctcggc gacaatgctt tcggtgccga taatgtcgga
     5101 atagcgcgac gggtgaatgc tgagcatgat caccatcggc gtcggctgca cggccgtgtg
     5161 cgcgatttcg aagcctacct tgatttccat gtcaagcctc cgctcccatg tccaccgaaa
     5221 cgacgttgat gctgacctcc atgccgacat acgcatcggc cgaaccgaga taggtgccgt
     5281 gcagcgggac ggcctggcgc ggatcgcgcg ccaccgccac gcgaacgaga tccttggtgc
     5341 cgacgatgcc gttggtcgga tcgaactcga tccagccggc gctcggcagg taggcctgaa
     5401 cccaggcgtg ggtcgagccg ccgccgacat agccatgcga ccgatcgccc ggaatgaaca
     5461 gatagcccga cacgaagcgc gcggcgatgc cgaggcgccg cagcgcctcg atcatgaaca
     5521 gcgcgtagtc gcggcaggtg ccggacctgg tctgcagggt gtcgagcgga tgctgggtgc
     5581 cgtgctcatg ccgcttgcgg taggtgaacg ccttgcggat gccgtgggtc atatcgctga
     5641 gaatattgaa agtcggggtc ggtccctcct cgtcgagaaa attccgcgcc cacgccgaca
     5701 attcgccgtc gggatcgcca tattgcggcg tgatgaattg aatgagatcg ggaaactcct
     5761 catcattgta gaaaaacgga tagaaatacg ccggatcgtc cggggtcggc acgtactcct
     5821 cggccggatt gtgctcgacg gtcgcggtag agctgaacgt cagggtttcg gcgcgctcgt
     5881 cgaaggtcgc aatcgcgacg ctgttgccga acacgtcatg gatccagcgc agcgacatcg
     5941 gctccggcac gatctcgaga ctgcccgaca gcacccgcag atcatggccg tctcgcgggc
     6001 gcagcatgat gcgatgttcg ccgaacgcca ccgggtgggc gtagcgatat tccgtcttgt
     6061 gatggatcgt cagcaggggc atcgtcgaag caatcatcgt gattttgtgc gatctaatct
     6121 ataccgttcc ctgcttattt cgaaatcggt atgctgcttc gctaggcaat agttgaggaa
     6181 aaatgacctt ggataacgcc ggcgacacat ctttactcct tggaagcaca gatgttccgc
     6241 cggttcttga ggataatgcc gcgggcggct cgccgttcct cttgacctgc gatcattacg
     6301 gccggttgac cccgcggagc ctcggcgatc tcgggctgcc ggccagcgag ctgacacggc
     6361 atatcgcctg ggatatcggg attgccggcg tcgccgaggc gctatcgaag cagctcgacg
     6421 cgcatctgat cgcgcagcgc tactcgcggc tggtgatcga ctgcaatcgc ccgcccggcg
     6481 cgccaagttc gatcccgcgc atcagcgagg cgaccacgat accggccaac gaagcgctta
     6541 gccgcgatgc ggccgaggtg cggcgccagg cgatcttcga tccctaccat cgccgcatcg
     6601 ccgaggtcat cgaggcgcgg ctgcgcgaag gcgttccgac ggtgctggtg tcgctgcaca
     6661 gctttacgcc ggtttacgcc ggcatcgccc ggccctggca catcggcacg ctctatcaac
     6721 gcgacatcag gctgccacag ctcctgctca agcagcttcg cggcgaaggc gatctggtgg
     6781 tcggcgacaa cgagccctat gccgtgagcg acgagaccga ctatacgata cccgtgcacg
     6841 gcgaggcgcg cgggttgatg aacacgggaa tcgaaattcg ccaggacctg atcgccgatc
     6901 aggccggcga gcagcaatgg gccgatcggc tggcgcggat ttttggcgag atcgaaacag
     6961 cgttgcgggc gcagcggctg gtgtagctgc taatcgtttc accgcgcccg cgtcagtgcc
     7021 agccagacgc cgc
//
LOCUS       DASWGM010000416         4878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_997736,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000416 DASWGM010000000
VERSION     DASWGM010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997736"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VGA15_12155"
     CDS             <1..339
                     /locus_tag="VGA15_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9588478.1"
                     /translation="MGVLATIGATSPFVGLFGTVWGIMNSFIGISKSQTTNLAVVAPG
                     IAEALLATAMGLVAAIPAVIIYNHFFRVTKGYLELVGRSSGAAGRLLSRDLDRTHVSS
                     AGSAPSRAAE"
     gene            489..1652
                     /locus_tag="VGA15_12160"
     CDS             489..1652
                     /locus_tag="VGA15_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HEX9588479.1"
                     /translation="MRMTRMKTNRQIALSCLAIIAATMVVDGALRSALAQNPFGGPPA
                     APDAQVGGIVGWLLTKQSEFYREMSSTIRAAKADGSAVWTLLAISFGYGMFHAAGPGH
                     GKTVISSYLVANEETARRGIVLSFVSALMQSLIAVLIVGIGAWLLNVTAKTMCGAERV
                     IEIASYTLIAAFGVRLVWTKGGGLIRALRSSQGKSGPRLAPVLAHAAAIQHHHGHHSH
                     GHGDHGHHHHAHAAAHPTVARDHVPGHEHAEHHDHSHVHDEHCGHSHGPEPAQLAGPG
                     GWRRGFSAVLTVGIRPCSGAILVLVFALAQGMFWAGIAATFVMGLGTAITVAAIAILA
                     VSAKGLARRIGAGREGGGALLLRGVEFGAAGLVLLFGVGLLLGYIAAERVTCF"
     gene            1959..2447
                     /locus_tag="VGA15_12165"
     CDS             1959..2447
                     /locus_tag="VGA15_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588480.1"
                     /translation="MANVVNNEIACRSRVGGVPSLGLVIGLSLFSFPAAAQVYPVNGV
                     WTAIDSQFPTAANETCIAVKMFGVEAVSKKSVSQMIIFAKDKRYDVKGDVQIETTIKS
                     IKVADGGFRITESFGKRGSWLGFRKKITYVLKVVDPLTIEIWDAARMTRYAKCGSQRP
                     PI"
     gene            2928..3386
                     /locus_tag="VGA15_12170"
     CDS             2928..3386
                     /locus_tag="VGA15_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588481.1"
                     /translation="MVFRWFKSEKRERRRKVKLDRKHLEARSRRFLKSYLNADETRKP
                     QFYRAVEEASKLCQPVKSGLPPPELEDAQIAEATSGAAMQMVLAREERGALKKDGRIS
                     DFVTDAYATVGIAYHRAAGVYTMDKEMQDLGTAAVHLLTMATSYMRAQND"
     gene            complement(3514..>4878)
                     /locus_tag="VGA15_12175"
     CDS             complement(3514..>4878)
                     /locus_tag="VGA15_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH family hydrolase"
                     /protein_id="HEX9588482.1"
                     /translation="WDRRQTPPPVREDFSKAPPPEKRETVPERQILVLGDAMADWLAY
                     GLEEAYSEQPDMGVVRKHKTVSGLIRYQPKGDPADWAAAAKGVLAIEKPDAIVVMLGL
                     DDRMPMREPAAEKTDGKSAADNKVDKKDNKKDAKAKPGGKPTGKPDDKPADAELSPGD
                     APGADQPPVIVPEKRARSPDGVYEFREERWVELYNKKIEEMIGVLRSKGVPVLWVGLP
                     VVRGPKATADTLFLDSLYRDAAGKAGITYVDVWDGFVDEAGRFLQQGPDFEGQIRRLR
                     SYDGVYFTKAGARKLAHYVEREINRLLAARSAPIALPTEPATPDANALPGQPAPRPLA
                     GPILPLVDSSVGTDQLLGGPGSRPAAVDALAARVLVKGEPLPAPAGRADDFVWPRREV
                     RREQARGETPVASASPDGGVPVTAPASLKPKKHRRQSGGQGHLSMGTRGMAREPALAR
                     SNLW"
BASE COUNT          942 a         1421 c         1504 g         1011 t
ORIGIN      
        1 atgggagtgc ttgcgacgat cggcgcgacc tcaccgtttg tcgggctgtt cggcacggtg
       61 tggggcatca tgaacagctt tatcggcatc tcgaaatcgc agaccaccaa tctggccgtg
      121 gtagcacccg gtattgccga agcgctgctc gccaccgcga tgggcctggt cgcggcgatc
      181 ccggccgtca tcatctacaa tcattttttc cgcgtgacca aaggctacct cgaactggtc
      241 ggccggtcgt cgggcgccgc cgggcgattg ctgtcgcgcg atctcgatcg cacccatgtg
      301 agttctgctg gcagcgcgcc ttcgcgcgcg gcggagtagc cgatcgcgca tctgtcgcgg
      361 caggtcgaag cccttgctct tatccttgag gatcggtgga ttgatggatc gcttccaaag
      421 gaccaaggct ccggcagcaa cagcaatgat ggcgtgatgt tcgccaacaa aatcaccgtg
      481 gattgtccat gaggatgacg cgcatgaaaa cgaaccgaca gattgcgttg agctgccttg
      541 cgatcatagc ggccacgatg gtcgtcgatg gcgctctgcg gagcgcgctg gcgcaaaatc
      601 cgttcggcgg tccacctgcg gcccccgacg cgcaagtagg cggtatcgtc ggctggctgc
      661 tgaccaagca atccgaattc taccgcgaga tgtcatcgac cattcgcgcc gccaaggctg
      721 acggcagcgc cgtctggacg ctgcttgcaa tttcgtttgg ctacggcatg ttccacgcgg
      781 cgggccctgg ccacggcaag acggtgatct cgtcatacct tgtcgccaat gaagagaccg
      841 cgcgacgcgg tatcgtgctg tcgttcgtct cggcgctgat gcagtcgttg atcgcggtac
      901 tgatcgtcgg catcggcgcc tggctgctca acgtcaccgc gaagaccatg tgcggcgccg
      961 agcgcgtgat cgagatcgcc agctacacgc tgatcgcagc tttcggtgtc cggctggtct
     1021 ggaccaaggg cggcggattg atccgggcgc tgcggtcatc ccaaggcaaa tccgggccac
     1081 ggcttgcgcc ggttctggcg cacgcggcgg caattcagca tcatcatgga catcactcgc
     1141 acggtcatgg cgatcacgga catcatcatc acgcccacgc cgctgcgcac ccaaccgtcg
     1201 ctcgcgatca tgtgcctgga cacgagcatg cggagcatca cgatcacagc catgtccacg
     1261 acgagcattg tggacattcc cacggtcctg agcccgcgca actcgccggt cccggcggct
     1321 ggcggcgtgg tttcagcgcg gttctcaccg tcggcatccg gccatgttca ggcgccattc
     1381 tggttctggt gttcgcgctg gcgcagggaa tgttctgggc cggcatcgcc gccacgttcg
     1441 tgatggggct tggcaccgcg atcaccgttg cggccattgc catccttgcg gtctccgcaa
     1501 aaggattggc gcggcggatc ggcgccggcc gcgagggcgg cggtgcactg ctgctgcgtg
     1561 gcgtcgaatt cggggccgcc ggactggtgc tgttgtttgg cgttggattg ctgctgggct
     1621 acatcgccgc cgagcgcgtg acctgctttt gagaggcgaa cgtcgcgcct tcggtccaaa
     1681 aaccgacttc cagggagcgt cgcctcttag cgcaaagcga ccatcgacgt cgtcggcgca
     1741 aatagcctaa cgccaaccac ggggtgtcgg cagtagcgcg accatggcaa gaaaacttag
     1801 tcaccatagg cttcggcaca gtcggtatag cgatgtggta tcatcgattc cgtcttgtgg
     1861 gggttgcgcc ggatcgcagt tacgatccat tgaattcagc aatatttgca atttttgtgt
     1921 tatattgaac gctgggggtt cgatggtgca ggaccctttt ggcgaacgta gtcaacaacg
     1981 aaattgcttg ccgttcccgc gttggtggag tgccttccct tggccttgtg atcggcttga
     2041 gtctattttc gtttcccgcg gcggcacagg tttatccggt caatggggta tggacggcca
     2101 tcgattctca gtttcccacc gccgctaatg aaacctgtat tgctgttaaa atgttcggcg
     2161 ttgaagccgt gtccaagaaa tctgtttcgc agatgattat ctttgcgaag gataagcggt
     2221 acgatgtcaa aggggatgtc cagattgaaa caaccatcaa atcgatcaaa gtggcggacg
     2281 gaggttttcg gataactgag tcgttcggta agcgcggaag ttggcttggg ttcaggaaaa
     2341 aaatcacata cgtcctgaag gtggttgacc cgctgacgat agagatttgg gatgcagcgc
     2401 gcatgacgcg ttacgcaaag tgtgggtctc aaagaccgcc gatctagcac tgatatatcg
     2461 acggattctg tgccatattt gtttgacata gatatcatcg aaagaaatgc ccttcgggcc
     2521 gctgccgagt tgccgttgct cgacgttcag gctgagatag cgcggctgaa tgcattccgc
     2581 gaagagaact acttgatcga cgatttgagc agtggcagcc gaactgtgcc acgaatggtg
     2641 cggcaaccgc gatggttggc tcgcgaacta tggccgatgg tcgctgcagc cagcggatga
     2701 gtgaagaaat acagaaaggt taactggatc cgggcgagcg agcggccatc aaagcaagag
     2761 ctgcaggtgt ctatgagggc ggaccgccct ctgtcgatcg cgaaagtcag agacctaaag
     2821 aaaagcggct tgggcgcctc ggaaatagcg aaggcaccga aaattggccg ggcgtcggtt
     2881 tatcgagcgc tactatctgc gccgtgaggc agtgggagcg aaacgaaatg gtgtttcgct
     2941 ggtttaaatc cgaaaagcgg gagcggcgga ggaaagttaa gcttgatcgc aagcaccttg
     3001 aagcaaggtc gcgccgcttt ctgaaaagct acctaaacgc ggatgaaacg cgaaagcctc
     3061 aattctatcg ggcggtcgaa gaggcgtcta agctatgcca gccagtgaaa tcaggtttac
     3121 cgccccccga gttggaagat gctcaaatcg ccgaagccac ttccggtgca gcgatgcaaa
     3181 tggtcttggc gcgtgaagag cgcggtgcgc tgaagaaaga tggccgcatt tccgactttg
     3241 tcaccgatgc ttacgctacc gtaggcattg cataccatcg cgcagcaggt gtctacacga
     3301 tggacaaaga gatgcaggat ttgggaactg ctgctgttca ccttctcacg atggcgacct
     3361 catatatgag ggcgcaaaac gactgacggc gagttaaccg gttccgaggc cgttcatcgg
     3421 aatcattcga ttgaggtgat gcagcttgat ctacatctta atgtatcgcc aaaccgtctt
     3481 tgccggtgcg gtggatatcc tccaccaggc cgcttaccac aggttgctgc gcgctaaagc
     3541 aggctcccgg gccatccctc tggtgcccat cgaaagatgg ccctggcccc cactctggcg
     3601 gcgatgcttt ttcggcttta gcgatgccgg cgccgttacc gggacgccgc catcgggcga
     3661 cgccgatgcc actggcgtct cacccctggc ctgttcgcgt ctgacttcgc ggcgcggcca
     3721 gacaaaatcg tcggcgcgac cagccggtgc cggcaaaggc tcacctttga ccaagacccg
     3781 cgctgcgagc gcatcgaccg ccgccggccg cgaccccgga ccgccgagca attgatcggt
     3841 gccgacagag gagtccacca ggggaaggat cggtccggcc agcggacgcg gcgccggctg
     3901 acccggcagc gcgttggcgt cgggcgttgc cggttcggtc ggcagcgcaa tcggcgcgga
     3961 acgcgcagcc aacagccggt tgatctcgcg ctcgacatag tgtgcaagct tgcgcgcgcc
     4021 tgccttggtg aagtatacgc cgtcgtaaga gcgcagccgg cggatctggc cttcgaaatc
     4081 gggaccttgc tgcagaaagc ggccggcttc atcgacaaag ccgtcccaga catcaacata
     4141 ggtgatgccg gccttgccgg ccgcatcacg atagagcgaa tcaaggaaca gcgtgtcggc
     4201 ggtcgccttt gggccacgta ccactggcag accgacccac agcaccggca cccccttgga
     4261 cctaagcacg ccgatcatct cttcgatctt tttattgtag agttcgaccc agcgttcctc
     4321 gcgaaactcg taaacgccgt caggcgagcg cgcacgtttc tccggcacaa tgactggcgg
     4381 ctgatcggcg ccgggggcat caccgggtga tagttcggca tctgcgggct tgtcgtcggg
     4441 cttcccggtg ggcttgccgc ccggtttggc cttcgcatcc ttcttgttat ctttcttatc
     4501 gaccttgttg tcggcagcgg atttgccgtc ggtcttctcg gccgcgggct cccgcatcgg
     4561 catacggtca tcgaggccta gcatgacgac aatagcgtcc ggcttctcga tggctaggac
     4621 gcccttggcc gcagcggccc agtcggcggg atcgcccttg ggttgataac gaattagacc
     4681 ggaaacggtc ttgtgcttcc gaaccacgcc catgtcgggc tgctcagaat aggcctcttc
     4741 gagaccgtaa gccagccagt ccgccatggc gtcaccgagc accagaatct ggcgctccgg
     4801 tacagtttcg cgtttctccg gcggcggcgc ctttgaaaaa tcctcgcgca cgggcggtgg
     4861 cgtttgccgc ctatccca
//
LOCUS       DASWGM010000417         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_45434,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000417 DASWGM010000000
VERSION     DASWGM010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45434"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..510)
                     /locus_tag="VGA15_12180"
     CDS             complement(358..510)
                     /locus_tag="VGA15_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588483.1"
                     /translation="MPRRSDELRALLRPFPAERIQAHEIGARIGNVKNDDAALIEWAS
                     AAQLPG"
     gene            complement(658..1086)
                     /locus_tag="VGA15_12185"
     CDS             complement(658..1086)
                     /locus_tag="VGA15_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588484.1"
                     /translation="MRRFFIALVTSLLALLPAMAADQGPDGTIELSGGSVAVGIGYSW
                     GSGKLVFQGKQYPLKVEGLSIADVGVSDYTATGSVYDLKKLQDIEGTYAAATAGATVG
                     GGVSGAAMRNQNGVLIQLSATRQGLQFTLAPAGIKLSLVK"
     gene            complement(1353..2690)
                     /locus_tag="VGA15_12190"
     CDS             complement(1353..2690)
                     /locus_tag="VGA15_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011992959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX9588485.1"
                     /translation="MSKSFRPWRIDETQLLPASVQDFVGKDHLARLVVSLVREQLSLA
                     EISASYSGEKGQPPYDPAMMTALLLYSYCSGVYSSRRIAKACRERTDFLMIVALDPPD
                     FRTVSEFRRRHLAALSGLFIQVLRLCETAGLVKLGHVALDGTKIQANASKHKAMSYER
                     MTRREKELAAEVARWLAAAEAADAAEDRAYGKDKRGDELPDWVADKAKRLAKIREAKA
                     ALEAEAKAAAEAKARADAAAEEQRRAAGRKKPGKKARPPSGAPDGKAQRNFTDPESRI
                     MKSKDGFVQAYNAQAAVDGTAQVIVAHGLTNCQSDQDQLAPRLDDIQAHLGRRPKEAS
                     ADAGYCSEANFAALEERNITGYVAVARAKHPDEAKRQPLRPLTQAMQRRLKRGGWRSR
                     YRLRKQIVEPVFGQIKAARGFRQFLMRGIEKVTAEWAIICTAHNILKLAKAYP"
     gene            complement(2758..>2911)
                     /locus_tag="VGA15_12195"
     CDS             complement(2758..>2911)
                     /locus_tag="VGA15_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007816440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV"
                     /protein_id="HEX9588486.1"
                     /translation="AVFMHMAWERLALMYAILVPPVLVLVFVGTMTFESDYTLFTRVR
                     FFGAGS"
BASE COUNT          484 a         1000 c          848 g          579 t
ORIGIN      
        1 gcctcggcct ctgcacgccg gaaaagaggc gatcctcaac tcgacttatc cgaccggttg
       61 cacctactct cgtaagcgaa acctgccgat ccggcgcgcc gcttcacggt ggtcgccggc
      121 ggcaaggggg atgacgggcc gcctggcact tcgtaaggtt gcgcgatggg cttccacttc
      181 agccgcgccc ttcgcctcgc ggtccgcgtt ttctggatcg ccgtcttcgt cgcggtattc
      241 tttctgttca gcggcgcgct cgactaagat tcaccgtaac ttcgacgctg agggcgattt
      301 gccggccgcc tgatccaggg aagccgggtc tttggcccga cccccccggc gggaccgcta
      361 tcctgggagt tgcgctgcgc tcgcccactc gatcagcgcc gcatcatcgt tcttcacgtt
      421 gccgatccgc gccccgatct catgcgcctg aatccgctcg gccgggaaag ggcggaggag
      481 tgcccgcaac tcgtcgctgc gccgcggcat gccacggtct gcgctcgact acggacggga
      541 aatcgacctt ttccgggcga tgtgtcgggc cgaggttgac tgctcggagg gtgcgagatc
      601 gcggccaatg atcgcaagcg agaccgatac gcggagacct taccgcgccg gccgaaccta
      661 tttcacgaga ctgagtttaa ttccggctgg ggccagggtg aactgaagcc cttggcgggt
      721 tgctgataac tggattaaga ccccattttg attccgcatg gccgcgccgc tcacgccgcc
      781 tcccacggta gctccggcgg tcgccgccgc gtaggtccct tcgatatctt gcagtttctt
      841 gagatcgtag acgctgcccg tggccgtgta atccgaaacg ccaacgtccg cgatcgaaag
      901 tccctcgact ttcagcgggt attgctttcc ctggaaaacg agcttgccgc taccccacga
      961 atagccgata cccactgcca ccgacccgcc tgataattcg atggttccat cgggcccctg
     1021 atcggctgcc attgcgggaa gcagcgccag caaactcgtt accaatgcga tgaaaaatcg
     1081 acgcatggct atctccattc gctgaatcgt ttgccacatc gtcactctgg ttatgcgtct
     1141 cgggatcatt tcttgccgtg atccttgaga ggtgaacgtt cgccacctct tcagcgtgcc
     1201 cttggggtga agcgcgtgcg cccggacttc cctcgcatag gtgcactgtc gacgagcaaa
     1261 ttcgaatgca atgcgacgct aggagcctgt ccgcttagtt atttgaacgg cggttttggg
     1321 gaacgaggga tagatcgtgg agcgacgctc tctcatggat aggcttttgc gagcttgagg
     1381 atgttgtggg cggtgcagat gatcgcccat tcggcggtca ctttctcgat accgcgcatg
     1441 aggaactgcc tgaagcctcg tgccgccttg atctgaccga acaccggctc gacgatttgc
     1501 ttgcgcaagc ggtagcggct gcgccaaccg ccgcgtttga gcctgcgctg catcgcctgg
     1561 gtcagcgggc ggagcggctg gcgtttggcc tcgtccggat gcttcgcccg ggcgacggcg
     1621 acgtagcccg tgatgttgcg ctcctcaagg gccgcgaaat tcgcctccga gcagtagccg
     1681 gcatccgctg acgcctcctt cggtcgccgg ccaagatgcg cttggatgtc atcgagccgt
     1741 ggggcgagct gatcctgatc gctctggcag ttggtcaggc cgtgcgcgac gatgacctgc
     1801 gccgtgccat ccaccgcagc ctgcgcgtta taggcctgaa cgaacccgtc cttgctcttc
     1861 atgatgcggc tttcaggatc ggtgaagttg cgctgcgcct tgccgtccgg ggcgccgctc
     1921 ggcggcctcg ccttcttacc tggcttcttg cgccccgccg cccggcgctg ctcttccgcc
     1981 gcggcgtcgg cccgcgcctt cgcctcggct gccgccttgg cttccgcctc gagcgccgcc
     2041 ttggcctcgc ggatcttggc cagccgcttc gccttgtcgg ccacccagtc tggcaactcg
     2101 tcgccgcgct tgtccttgcc gtaggcgcga tcctccgccg cgtccgccgc ctccgccgcc
     2161 gcaagccagc gcgccacctc cgccgccaac tccttctccc gccgtgtcat gcgctcatag
     2221 ctcatcgcct tgtgcttcga cgcgttcgcc tggatcttcg tcccgtcgag cgccacgtgc
     2281 ccgagcttca ccagcccagc cgtctcgcac agccgcaaga cctggatgaa caaccccgac
     2341 agcgccgcca aatgccgccg ccggaactcg ctcaccgtcc ggaagtcggg cggatccaga
     2401 gcaacgatca tcaggaagtc ggtccgctcc cgacacgcct tggcgatccg tcgcgacgag
     2461 tagacgccgc tgcaatagct gtacaacagc agcgctgtca tcatcgccgg atcgtacggc
     2521 ggctgaccct tctcgccaga gtagctcgcg ctgatctccg ccagcgagag ctgctcccga
     2581 accaggctca ccaccagccg cgccaaatga tccttgccaa cgaagtcctg caccgaggcc
     2641 ggaagcagct gcgtctcgtc gatgcgccag gggcggaagc tcttgctcat ggcaacaact
     2701 tgaatcagac ttccgcccca acgtcgacac caaaatcgcc attactcaga caggctccta
     2761 ggaccctgcc ccgaaaaatc tcactcgcgt gaagagcgtg tagtccgatt cgaacgtcat
     2821 ggttcccaca aacaccaaca ccaataccgg cgggaccaga atggcataca tcagggccag
     2881 ccgctcccag gccatgtgca tgaagaccgc g
//
LOCUS       DASWGM010000418         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1274905,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000418 DASWGM010000000
VERSION     DASWGM010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274905"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VGA15_12200"
     CDS             complement(<1..518)
                     /locus_tag="VGA15_12200"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX9588487.1"
                     /translation="MVDRDILIVGGYGVVGRRIASELGPDYPGRVVVAGRSRARADEA
                     ATAIGHGVLGRSIDIAVPSSIAASLDGVAVVISCIDQPGRTLLWAAVERGLRYTDITP
                     HLTELGRGAEYERIDTAARASGARLVLGTGIVPGISNVMVRALADALGGADEIETALL
                     LNAADVSGPASF"
     gene            complement(561..1232)
                     /locus_tag="VGA15_12205"
     CDS             complement(561..1232)
                     /locus_tag="VGA15_12205"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX9588488.1"
                     /translation="MALTEVDPALRSEPARPRASRAAAQLDLRKITTPILLHLLRGKI
                     RFPRLFLVKCKLTVNRFKKTIDPRFPAEMVDMCALPLWVYINLKRELGQKTAFEIMRV
                     AILTGGVAQWNFAYHAAEEDRTFKNLCDEEIIVNQSGPTRWNTLEVVERTERRFEIKI
                     TRCLYHELTTAMGIPELTPVICQVDNAAFNSYLPDEVVFSRGGPGHRISDGAKACNFK
                     WDFIG"
     gene            1482..1781
                     /locus_tag="VGA15_12210"
     CDS             1482..1781
                     /locus_tag="VGA15_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEX9588489.1"
                     /translation="MSKKTSMAAIAMIAAGLLSSAANAHPELQSAEPAAGAAMTTSPT
                     QIRITFNENVIPKFSGVELKDQTGKTIATGKAAEWHAVSDDTHRVKGSYSFSVAP"
BASE COUNT          395 a          577 c          547 g          367 t
ORIGIN      
        1 tcgaacgacg cgggaccgct gacatcggcc gcattcagca ggagcgcagt ttcgatctcg
       61 tcggctccgc caagcgcatc ggcgagtgca cgcaccatca cgttcgagat gccggggacg
      121 atgccggttc ccaacaccaa ccgcgctccg gacgcccgcg cggcggtgtc gatcctttcg
      181 tattccgcac cgcgcccaag ctcggtcaga tgcggcgtaa tatcggtata tcgcaacccg
      241 cgctcgacgg ccgcccacag cagcgtacgg cccggctgat cgatacagct gatgacgaca
      301 gcgacgccgt ccagcgaagc cgcaatggac gatggcacgg caatgtcgat acttcgtccg
      361 agaacgccat ggccaatcgc agtcgccgcc tcatccgctc gcgcgcggct gcggcctgcg
      421 acgacgacac ggccggggta atctggtccg agttctgacg ctatccgccg cccgaccacg
      481 ccatagccgc cgacgatgag aatgtcccga tcaaccaaat cgtggctcct caaagccttc
      541 ggcgcggaag gcttgcgcct tcatccaatg aagtcccatt tgaaattgca tgctttggcg
      601 ccgtcggaga tccgatgccc gggaccgccc cgcgagaaca cgacttcgtc gggaagatac
      661 gaattgaagg cggcgttatc gacctggcaa ataaccggcg tcagctcggg aattcccatc
      721 gccgtcgtga gttcgtggta gaggcatcgc gtgatcttga tctcgaaccg ccgttcggtc
      781 cgctcgacca cctccagcgt gttccagcgc gttgggccac tctgattgac gatgatctct
      841 tcgtcgcaga ggtttttgaa ggtgcgatcc tcctcggcgg cgtgataggc gaaattccat
      901 tgtgcgacgc ctccggtgag aatcgcgacg cgcatgatct cgaatgcggt tttctgcccg
      961 agttcccttt tcagattgat gtagacccac agcggcagcg cgcacatgtc gaccatttct
     1021 gcgggaaagc gcggatcgat ggttttcttg aaccgattga ccgtcaattt gcatttcacc
     1081 aggaacagcc gcgggaaccg aatcttgcca cgcagcagat gcaacaagat gggcgtggtg
     1141 atcttccgca aatcgagttg agcggccgcg cgcgatgctc gcgggcgcgc gggttcactg
     1201 cggagggcgg ggtcgacctc ggtaagcgcc atgttgttcc tccttcatcc gaaggtggcc
     1261 ttgaattgca atttgcaaaa atactggaaa ggcaatcgag cgccttgatc cagatcacgg
     1321 cccaaaatat ggccgaatat ccgcctatcg agaggcgggt tatttgctgg taaggcttga
     1381 taccaaaaaa cgatggggcc gctacgccgg agatctgatg ctcatcccgg atgtgcgctg
     1441 aaaattacga ttccagcaac tccgcaatca aaggaagtat aatgtcgaag aagacttcaa
     1501 tggccgccat tgcgatgatc gcggcgggcc tgctcagtag cgccgcgaat gctcatccgg
     1561 agcttcaatc ggcagagccc gctgctggcg cggccatgac gacgtcgccc acgcagataa
     1621 ggatcacatt caatgaaaac gtgatcccaa aattctccgg cgttgagctg aaggaccaaa
     1681 ccggcaagac gatcgcgacc ggcaaggccg cggaatggca cgcggtgtcc gacgacaccc
     1741 accgagtcaa aggcagttac tcgttcagcg tagctccctg atgatcgatc ttggactcgt
     1801 catcgcgcgg cttctgcact acgccgcggt gaccacgctc gccggtgtgt cattttttcc
     1861 gctgtacgcg tatgcggacc ccgaac
//
LOCUS       DASWGM010000419         7853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1981931,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000419 DASWGM010000000
VERSION     DASWGM010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1981931"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGA15_12215"
     CDS             <1..592
                     /locus_tag="VGA15_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9588490.1"
                     /translation="WVHAYYNAITKRLKKLADYEINPNMALVSFFMCAKTDEEARARA
                     DGATFFQFALRYYGQGQNRARPAPGTVNMWDEYNKWKRDNPEAQEAALRGGLIGSPET
                     IARKLRRFRSSHIDQVILLNQAGKNSHEHICDSLELFAKEVMPEFRADPEHDAWKAGV
                     TSGAIQLEEIDTEAFKDRYGKLAVNVAPAKAAVAAG"
     gene            complement(626..868)
                     /locus_tag="VGA15_12220"
     CDS             complement(626..868)
                     /locus_tag="VGA15_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588491.1"
                     /translation="MIPFYIVRHLAGEFEELSCFRQLEDESAALYFNIVHGAKVRRRF
                     TTQRSGERCADYVLVARATGAGGFIEEREIALFDAS"
     gene            1388..2125
                     /locus_tag="VGA15_12225"
     CDS             1388..2125
                     /locus_tag="VGA15_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9588492.1"
                     /translation="MTTLQRTAIVVGVGSEQGLGAAIARRFAVEGQRVIVSGRTEAKI
                     AQVVRAIVDSGGNAEAFTADATIESEVVALFDFAGASGHPIDLVVFNAGNNVRHDFRT
                     MPAELFEQTWRVATFGGFLVGREAARRLAPSRQGTIIFTGATASLRGKPPFTAFASAK
                     AGLRSLAQSMAREFGPLGIHVAHVVIDGGIDGEKLNTAAPQLRIERGADGLLNIDAIA
                     AAYWHLHGQHRSAWTHELDLRPFKERF"
     gene            2159..3001
                     /locus_tag="VGA15_12230"
     CDS             2159..3001
                     /locus_tag="VGA15_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX9588493.1"
                     /translation="MGLQNEKIAALSFVTADLNYLSPGLDRPRTFTFEPPSGEPRSNI
                     VPEPHSLPVHDIRPIGETVSLDREGFALVRQNSEVNDFYDEDKVRSVYYPEAERLIKA
                     ATGADRVFVFDHTVRKRVQGAADRDGGLRQPVARVHVDHTERSGPQRVRDLIPDEAEE
                     LLKGRVQIINLWRPIRGPLLDSPLAVCDARTVRPNELVAADLVYPNRVGETYSVKYNP
                     DHQWFYVPKMTADEVLLLKCFDSRTDGRARFAPHSAFIDPTTPPDAPPRESIELRTLV
                     FHRN"
     gene            complement(3065..3952)
                     /locus_tag="VGA15_12235"
     CDS             complement(3065..3952)
                     /locus_tag="VGA15_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588494.1"
                     /translation="MAADPVTLRVGDQKGGNRSLLEIAGLAKDLPYKIEWSEFPAAAP
                     ILEALNAGALDVGYTDDLAFLTVYAAGAPIKAIGGTRADARTQAILVRQDSPIKTAAD
                     LKGKRLAGTRGGWGQFLIDATLEKAGHKIDDATFAPLGPVDAKIALVAGSIDGWAVWE
                     PYVSYATLKDNARIIADGEGLTPTITFLVASDQAIATKRAAVQDLVQRLNTARLWSLD
                     HLPEYAKSTADLTKLPEDVLLSAYTAQRTSPIVIDEGIIREVQEASDRATRYGILAKT
                     LDVSKAVDRSFTAASAGSN"
     gene            4541..5641
                     /locus_tag="VGA15_12240"
     CDS             4541..5641
                     /locus_tag="VGA15_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588495.1"
                     /translation="MSRDMKWDDALLDRRTLLRAGAVAAFAAPLGVFGAQAFPFRSAT
                     PNIDFSEFPICKASSDAAPLAGAPRKLKLSWNAGAVCLAPLPVAIDHGFFQKQNLDVE
                     LVNYSGSTDQLLEAIATGKTDAGLGMALRWLKPLEQGFDVKIAAGTHGGCMRVLSRTD
                     SGVDQLADLKGKIVAVGDLAGPDKNFFSIQLARQGIDPNKDVDWRAYPGNLLQLAVEK
                     REVQAFLASDPIAYLWLKDSAYKEVASNLDGDYKDKSCCIVGLRGSLVREEPQVARAI
                     TQALLDAAMFTSQNPDKAAASFQPYAPKTASLQDLQAMVRYHTHHHHPVGAVLKQELK
                     AYADDLKLVSVFKPSTDTAKFAERIYVDVFGV"
     gene            5619..6659
                     /locus_tag="VGA15_12245"
     CDS             5619..6659
                     /locus_tag="VGA15_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9588496.1"
                     /translation="MSTYSASKTVALPQSAEEGAAFAGRPLSLPTSFLASGTAVSATA
                     AWIGFGLSCLLWEDLGDWSRTHALAIAAFIIAGGIIAATASGKWLGRFGTGLQARAPW
                     LLALGLFLTLWELATAKFAWLPLPFFPPPQAIIEVYTDDLPKLLDSVFASVKLQLGGY
                     AIGAAAGFLTGVSIGWSRSVGYWVHPVLRFIGPLPATAWLPFAFFTFPSSWSASTFLI
                     ALATGFPVTVLTWSGMASVNNAYYDVARTLGAKPSFLVLKVAIPAALPHVFVGLFMGL
                     GSSFAVLVVAEMIGVKAGLGWYLQWAQGWAAYSNMYAALIVMSLLCSGAITLLFRTRD
                     RLLVWQKGVVKW"
     gene            6653..7450
                     /locus_tag="VGA15_12250"
     CDS             6653..7450
                     /locus_tag="VGA15_12250"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588497.1"
                     /translation="MVALASAERAAPAVAGASLDVEAVSHAFDIDGAVLPVLDDISFS
                     VKPGEFVALLGPSGCGKSTLLRLVAGLEPPRSGGLREDGDPITGPFPSRVVVFQDPTL
                     FPWRKVWDNVALGLEAQGILRTRRHRVDAAIDLVGLSTFRNAYPHQLSGGMAQRVALA
                     RALVNDPKILILDEPLGKLDSLTRITMQAEIASLWQRSGFTTLLVTHDVEEAVFLANR
                     VIVFSDRPARIKADIAVDRPYPRHRGDPILAELRRNILGLLGLDATW"
     gene            7444..>7853
                     /locus_tag="VGA15_12255"
     CDS             7444..>7853
                     /locus_tag="VGA15_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9588498.1"
                     /translation="MVSAPGNSMRKEFFTDATPAIFDCAKLLASEFAATAALHDREAS
                     FPFENFNQLSEAGLLALTVPTALGGSGAGALDAARIINIFGKADPSTALVLSMHYIQH
                     LVMARSTRWPGRLARKLARETVEGVALINALRVEP"
BASE COUNT         1487 a         2376 c         2403 g         1587 t
ORIGIN      
        1 ctgggtgcac gcctattaca acgccatcac caaacggctg aagaagctcg cggactacga
       61 gatcaatccg aacatggcac tggtatcgtt cttcatgtgc gccaagaccg acgaggaggc
      121 gagggcgcgc gccgatggcg cgacgttctt ccagttcgcg ctgcgctatt acggccaggg
      181 acagaaccgg gcgcgtcccg cgccgggcac ggtcaacatg tgggacgaat acaacaagtg
      241 gaagcgcgac aaccccgaag cgcaggaggc ggcgctgcgc ggcggcctga tcggatcgcc
      301 cgaaaccatc gccaggaagt tgcgacggtt ccgcagctcc catatcgacc aggtgatcct
      361 gctcaaccag gccggcaaga acagccacga gcacatctgc gactcgctcg aattgtttgc
      421 caaggaggtg atgccggaat tccgcgccga tcccgagcac gatgcatgga aggccggcgt
      481 gacgagcggc gccatccagc tcgaggaaat cgataccgaa gccttcaagg accgctatgg
      541 caaattggcg gtgaacgtcg cgcctgccaa ggcggccgtg gcggcgggat aacggttttc
      601 tcccgggcca tgcgggattg ctcgatcacg aagcatcgaa cagcgctatt tctctttctt
      661 cgatgaaccc gccggctccg gtcgctctcg cgaccagcac atagtccgcg catcgctcgc
      721 ccgacctttg ggtggtgaac cgcctgcgga ctttcgcgcc gtggacgata ttgaaataca
      781 acgccgcgct ttcgtcctcc aattgtcgaa aacaggagag ttcttcgaac tctcctgcga
      841 gatgacgcac gatatagaac ggtatcatgt ttggtcgagc ccggtcggtg gcgttgcttc
      901 tagtgagaca tccagaccag cccggccaat accgatatct ggccaataac cagcgctgcc
      961 attttgatcg gatagagggt gcgtcgttga atcatctgcg atctgacgat gaagttcaca
     1021 tggccgtgat acgcgtcatc tccaaaagcg aggatgcaca attatggtca ctccagcact
     1081 attttgaatt ccaaagttga ttggattgtt cgcacaacat tctccaaggg ccaccagcga
     1141 aagacgataa ttgagcggcc tgctctgatc cgaagatgtt cgcatttcag gttcgaggta
     1201 gccggcctcc tgactcaaca aggacttcac tccgcctgtc cttctccttg gaagaatttt
     1261 tcacggcaca cacgaattgc gcgacagcaa tcttattttt cgcgggccat tggcgggact
     1321 acctggtcga tacagattta gcctcgtagt ttttccgaga ttgaatcgga ccggggagat
     1381 accagcaatg accacgcttc aacgcacggc tattgtcgtg ggggtcggtt ccgaacaagg
     1441 actcggcgcc gcgatcgcgc ggcggttcgc tgttgaaggt cagcgggtca tcgtttccgg
     1501 gcgcacggaa gcaaaaattg cgcaggtcgt tcgcgccatt gtcgacagcg gcggcaacgc
     1561 cgaggctttc accgccgacg ccacgatcga gtcggaggtg gtggcgctgt ttgatttcgc
     1621 cggggcttcc ggtcacccca tcgacctcgt ggtgttcaac gcgggcaaca acgttcgcca
     1681 tgacttccgt accatgccgg ccgagctctt cgagcagacc tggcgtgtcg cgacttttgg
     1741 cggatttctg gtggggcggg aagccgcccg gcggttggcg ccgtcacgtc aaggcacgat
     1801 catctttaca ggcgccacag ccagcctgcg cggcaaaccg ccgttcacgg cgttcgcatc
     1861 cgccaaggcc ggccttcgct cgctggcgca atcgatggcg cgcgaattcg ggccgctcgg
     1921 aattcacgtc gcgcatgtcg tcatcgacgg cggcatcgat ggcgaaaagc tcaacacggc
     1981 agcaccccaa ctcaggatcg aacgtggcgc cgatggactg ctgaacatcg atgcgatcgc
     2041 cgcggcctat tggcatcttc acgggcagca ccggtctgcc tggacgcacg aactggatct
     2101 gcgaccattc aaggagcgtt tctagaagcc ggaccagcca acaaaaaagg agaagaacat
     2161 gggactgcaa aacgaaaaga tcgcggcgtt gtcgttcgtc acggccgatc tcaactatct
     2221 gtcgcccggt ctggacaggc cgcgaacctt tacctttgag ccgccgtccg gcgagcccag
     2281 gtccaatatc gtgcccgagc cgcacagcct gcccgttcat gatattcgcc cgatcggcga
     2341 gacggtttcg cttgatcgag aagggtttgc cctggttcgg caaaacagtg aggtcaacga
     2401 cttctacgac gaagacaagg tcagaagcgt ctattatcca gaggcggaac ggctgatcaa
     2461 agccgcgacc ggcgctgacc gggtattcgt cttcgatcac accgtacgca agcgtgttca
     2521 aggcgcggcc gaccgcgatg gcggcctgcg tcaaccggtt gcgcgcgtgc atgtcgatca
     2581 caccgaacga tcaggcccgc agcgcgtccg cgatctcatc ccggacgagg ccgaagaact
     2641 gctgaagggc agggtgcaga tcatcaatct ctggcgaccg attcgaggcc cgttgctcga
     2701 ctcgcccttg gcggtctgcg atgcccgcac ggtaaggccc aacgaactgg ttgctgccga
     2761 tctggtctat cccaaccgcg tcggagaaac ctattcggtg aaatacaatc ccgatcatca
     2821 atggttctac gtgccgaaga tgacggccga tgaagtcttg ctgctgaagt gctttgattc
     2881 caggaccgac ggacgcgccc gctttgcgcc ccatagcgcg ttcatcgatc cgaccacacc
     2941 gcccgatgcg ccgccgcgcg agagcattga attgcgcacc ctggtgtttc acaggaatta
     3001 aagcgcaaga gagagggccg gcatcggatg ccggcccttt gcctttggcc tggaaccagc
     3061 cgcgtcagtt cgatccggcc gatgccgcgg taaaactgcg atccaccgcc ttgcttacat
     3121 cgagcgtctt ggcaaggatg ccatatcggg tggcacggtc ggaggcctcc tgaacttccc
     3181 tgataatgcc ttcgtcgatg acgatcgggc tggtccgctg cgccgtgtat gcgctgagca
     3241 gcacgtcttc cggcagcttg gtcaagtcgg cagtgctctt ggcatattcg ggcagatgat
     3301 ccagcgacca cagccgcgcc gtgttcagcc gttgaaccag gtcctgcacg gcggcgcgct
     3361 tggtggcgat ggcctggtcg gaggcgacga ggaatgtgat ggtcggtgtc aggccctcgc
     3421 cgtcagcaat aatccgcgca ttgtccttga gcgtcgcgta ggacacgtag ggctcccaga
     3481 ccgcccatcc atcgatggag ccggccacca gcgctatctt ggcatcgaca ggacccagcg
     3541 gcgcgaatgt ggcgtcgtcg attttatgcc ctgccttttc cagcgtcgcg tcgatcagga
     3601 actggcccca gccgccccgc gttccggcca atcgcttgcc cttcaaatcg gccgccgtct
     3661 tgatcggcga atcctggcga accagaatgg cctgcgtcct ggcatcggcg cgggtaccgc
     3721 cgatggcttt gatcggcgca ccggcggcat acacggtcag gaaagccagg tcatcggtgt
     3781 aaccgacgtc gagcgcgccg gcgttgaggg cttcgagaat cggcgccgcg gccggaaatt
     3841 ccgaccattc gattttgtaa ggaagatcct tggccagccc ggcaatctcg agcagcgagc
     3901 gattgccgcc cttctgatcg ccgacacgca gcgtaacggg gtccgctgcc atcgccgatg
     3961 cggtgatggc gaatccgatt gccaccccga acagcgtcgc gccagccttg cgaaggcatg
     4021 cgcgtaattc ggatctgggt ttgactccat tcatgctcgt tactctccgc ttgctttgct
     4081 gcgtcacttc aatcgatcaa cgatgccaaa agcctacgag taaccggatt gcagtcaatg
     4141 aaatggccaa ctgaatattg cggcgcccga gcaatgatcg tctcgatttt caagttgtga
     4201 tggagactgt ctgctgccca gcgtgaatac gcttgaaaca gtcggtatcg aatcgaagtt
     4261 ggagcgatgc gcatttgttg acacggcaaa aactgtttca tcgcgcaacg atatgatggt
     4321 ggagataatc tctccgttcg ctttcacatt caaaattcca tttcattgac gattgcgata
     4381 ccgccgtgca tcatttgaac gtcaattcaa cagagcgaga gttgaaatct cgccgagagt
     4441 gaaccgattt cacggacgcc agtgtgaaac agcgccgggc cgcaacgtca ccgcgttgcg
     4501 ccgacggtgg ccctatgccg cctcacagca ggagaagaag atgtcgagag atatgaagtg
     4561 ggacgatgca ttgctggatc gccgcacatt attgcgcgct ggcgccgtgg cggcgtttgc
     4621 ggcgccgtta ggcgtattcg gcgcgcaggc atttccgttc cgctcggcaa ctccgaatat
     4681 cgatttctcg gaatttccga tctgcaaggc ctcgtccgat gccgcgccgc tcgcgggcgc
     4741 gccacgaaag ctgaaattgt cctggaatgc cggcgcggtc tgcctcgcgc cgctccccgt
     4801 cgccatcgat catggtttct tccagaagca gaatctcgac gtcgagctgg tcaattacag
     4861 cggctcgacc gaccaattgc tggaggcgat cgcgaccggc aagaccgatg caggtctcgg
     4921 catggcgttg cgctggctga agccgctgga gcagggtttt gatgtcaaga tcgccgccgg
     4981 cacccatggc ggctgcatgc gcgttctgtc gcgcaccgac tcgggtgtcg accagcttgc
     5041 cgacctgaaa ggcaagatcg tcgcggtcgg cgatctcgca ggaccggaca agaatttctt
     5101 ctcgattcag ctcgcgaggc aaggtatcga tccgaacaag gacgtcgact ggcgggctta
     5161 tccgggcaat ctgctgcagc tcgccgtcga gaagagggaa gtccaggcct tcctcgcctc
     5221 ggatccgatc gcctatctct ggctgaagga cagcgcctac aaggaagtcg cctccaacct
     5281 cgacggcgac tacaaggaca agagctgctg catcgtcgga ttgcgcggca gtcttgtgcg
     5341 cgaggagccg caggtcgcgc gggcgatcac gcaagcgctg ctcgatgcgg cgatgttcac
     5401 gtcgcagaac ccggacaagg ccgcggcttc gtttcagccc tacgcgccca agaccgcgag
     5461 cctgcaggat ctgcaggcga tggttcgcta tcacacccat catcaccacc cggttggggc
     5521 cgtgctcaag caggagctga aggcctatgc ggacgatctg aagctcgttt cggtgttcaa
     5581 gccgagcacc gacacagcaa aatttgcgga gcggatttat gtcgacgtat tcggcgtcta
     5641 agacggttgc gttgccgcaa tcggcggagg agggcgccgc gttcgccggc cgtccgctgt
     5701 cgcttcccac gtcgtttctg gccagtggaa ccgccgtatc cgcaaccgcg gcgtggattg
     5761 gcttcgggct ctcgtgcctg ctgtgggagg atttgggcga ttggtcgcgt acccatgcac
     5821 ttgcgatcgc cgcgtttatc atcgccggcg gcatcattgc ggcgacagcg agcggtaaat
     5881 ggctcggccg tttcggaacg ggcctgcaag cgcgtgcgcc gtggctgctg gcactcggat
     5941 tgtttttgac cctgtgggag ctcgcgaccg caaaattcgc atggctgccg ctgccgttct
     6001 ttccgccgcc acaggcgatc atcgaagtat ataccgacga tctgccaaaa ctgctggata
     6061 gtgtttttgc ctccgtcaaa cttcagctcg gcggctacgc catcggcgcg gccgccggct
     6121 ttctgaccgg cgtgtcgatt ggatggtcgc gcagcgtcgg ctactgggta catccggtgt
     6181 tgcgattcat cgggccgttg ccggcgacgg catggcttcc gtttgcgttc ttcacctttc
     6241 cgtcgagctg gagcgcgagc acgttcctga ttgcgctcgc gaccggattt ccggtcacgg
     6301 tgctgacctg gtccggcatg gcgagcgtca acaatgccta ttacgatgtg gcgcgaaccc
     6361 ttggtgccaa gccatcgttc ctggtgctga aagtcgcgat ccccgcggcg ctgccgcacg
     6421 tcttcgtcgg cctcttcatg ggcctcgggt cgtcattcgc cgtcctggtc gtcgccgaaa
     6481 tgatcggcgt caaggccggt cttggctggt atctgcagtg ggcccagggc tgggcagcct
     6541 attcgaatat gtatgccgcg ctgatcgtga tgtcgctgtt gtgctcgggc gcgatcacgc
     6601 tgctgtttcg gacgcgcgac cggctgctgg tctggcaaaa aggcgtcgtg aaatggtagc
     6661 gcttgcgtct gccgaacggg ctgcacccgc cgtggctgga gcgtcgctgg atgtcgaggc
     6721 cgtcagccat gcgttcgaca tcgacggcgc tgtcctcccg gtgctggatg acatcagctt
     6781 cagcgtgaag cccggcgaat tcgtcgcatt gctcggtccg tccggatgcg gaaaatcgac
     6841 gttgctgcgc ctggtcgcgg ggcttgagcc gccgcggtcg ggcggcttgc gcgaggatgg
     6901 cgatccgatc acggggccgt ttccgtcgcg ggtcgtggtg tttcaggacc cgacattgtt
     6961 tccatggcga aaggtgtggg acaatgtcgc actcgggctc gaagcccagg gaatcctcag
     7021 gactcggcga caccgggtcg atgcagccat cgatctcgtg ggcctctcga catttcgcaa
     7081 tgcctatcca caccagcttt ccggcggtat ggcgcagcgg gtcgcactgg cgcgagcgct
     7141 ggtcaacgat cccaaaatac tcattctcga cgagcctctc ggcaaactcg actcgctgac
     7201 gcgcatcacc atgcaggcgg aaatcgcctc gctctggcag cgcagcggat tcaccacgct
     7261 attggtgacc catgatgtgg aggaggccgt gtttctggcc aatcgcgtga tcgtgttcag
     7321 cgatcggccc gcgcgcatca aggccgacat cgcggtcgat cgtccctacc cgcggcaccg
     7381 gggcgatccc atattggcgg aactgcgccg gaacattctg gggctgctcg gattggacgc
     7441 aacatggtga gcgcgccagg caactccatg cgcaaggaat ttttcacgga cgcaacgccg
     7501 gcgatcttcg actgcgcaaa actgcttgct tccgaatttg ccgcgacggc tgctctgcat
     7561 gatcgtgagg cgagctttcc gttcgagaat ttcaaccagc tgtccgaggc gggattgctg
     7621 gcgctgaccg tgccgacggc gctgggtggc tcgggcgccg gcgcgctcga tgcggcgcgc
     7681 atcatcaaca tcttcggaaa ggccgatcca tcgacagcgc tggtgctgtc gatgcattac
     7741 attcagcatc tggtgatggc gagaagcacg cgctggccgg gtcgtcttgc acgcaagctg
     7801 gcccgcgaaa ccgtcgaagg cgtggcgctg atcaacgccc tgcgggtcga gcc
//
LOCUS       DASWGM010000420         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_445939,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000420 DASWGM010000000
VERSION     DASWGM010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445939"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VGA15_12260"
     CDS             <1..238
                     /locus_tag="VGA15_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588499.1"
                     /translation="IGRAQILIDRLGFRRRLDNDDIHVIPMAYGENKGRFREIRVGRF
                     EGNMGVAGAPVKSTDVEGGREQGEPVSKKLKSAS"
     gene            395..538
                     /locus_tag="VGA15_12265"
     CDS             395..538
                     /locus_tag="VGA15_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588500.1"
                     /translation="MAFIAENVADLARLARRHNLEMLVRLLEMAQMEAQERIQLRGKR
                     NLS"
     gene            complement(557..1033)
                     /locus_tag="VGA15_12270"
     CDS             complement(557..1033)
                     /locus_tag="VGA15_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9588501.1"
                     /translation="MVKRTSLEKAECPIARSLDALGDWWSLLIIREAFLGKRRFGEFQ
                     KSIGLAKNILTVRLRALVERGILTTAPASDGSAYQEYLLTPKGRGIFPVLVALRQWSE
                     EFSCDGGGFPTLLVDRDKGRPVRKLELRAEDGRLLGNGDTEVRPNSGAKRRRRVLA"
     gene            1154..1927
                     /locus_tag="VGA15_12275"
     CDS             1154..1927
                     /locus_tag="VGA15_12275"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9588502.1"
                     /translation="MRLANKTALITGGNSGIGLATARLFVAEGARVAITGRNQATLEA
                     AAKELGPNALAIVADATDIAATEQAIRQAVEKFGKLDIVFANAGIPGNTPIGGTTLAA
                     FEQVIRTNITSVFFTVQAAAPHLNDGASIILNGSVISVLGNPGYSAYAASKAGVRAMA
                     RVMASELSPRGIRVNVVAPGAARTPIWNGAAPTAEAFAVLDKRISRTIPLGRLGEAEE
                     VAKTVLFLASDDASNVQGAEIFVDGGATGSPAGAPIYRG"
     gene            complement(2379..>2889)
                     /locus_tag="VGA15_12280"
     CDS             complement(2379..>2889)
                     /locus_tag="VGA15_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX9588503.1"
                     /translation="GLEIVYRDLTLTPLAHLSGSHLSAGQGAAPEAALQRDLAAGRAV
                     LDEFLAADIVVLGAPMYNFTLPSQLKAWIDRIVVAGKTFKYGAQGAEGLAGNKRVIIA
                     ISRGGFYGAGTPAAAGEHLETYLRWVFGFIGVTNPEFISADGIQVGPEHREKALANAL
                     QAATDLHAA"
BASE COUNT          565 a          867 c          898 g          559 t
ORIGIN      
        1 cataggccga gcccaaatac ttattgatcg tcttggcttt cgccggcgac tcgacaatga
       61 cgatattcat gtcattccaa tggcttacgg ggaaaataag ggccggttcc gcgagattcg
      121 cgtcggccgt ttcgaaggga acatgggtgt agcgggggcc cctgtcaaat cgacagatgt
      181 tgaagggggg cgggaacaag gggaaccagt atccaaaaag ttgaaaagtg cgtcctagag
      241 ttgcgctctc agaaagggta tccgtcacca atacgcggca ttggatgtgt tttaattgac
      301 caagacagga cgtcggcgga gggccgcatc ttcaggctgg gataagagtt cgcgaaccga
      361 tgatccgccg ggggacggcg ggccggatga ggctatggcc ttcatcgcgg agaacgtcgc
      421 cgacctcgcc cggttggcgc ggcgccataa tctcgaaatg ctcgttcgtc tgctcgagat
      481 ggcgcagatg gaagcgcagg agcgcatcca gcttcgcggc aaacgcaatt tgtcttaaat
      541 gtcaggctgg catcgctcag gccaaaacgc gccggcggcg tttggcgccg gaattcggcc
      601 gcacttcggt atcgccattg ccgagcaagc ggccgtcctc tgcacggagt tcgagttttc
      661 gcaccggtcg gcctttgtcg cgatcgacca gcagggttgg gaagccaccg ccatcgcagg
      721 aaaattcctc gctccactgc cgcagcgcga ccagcaccgg aaatatcccg cgccccttcg
      781 gggtcagcag atattcctga taggcgctgc cgtcggaggc gggcgccgtc gtcaggatgc
      841 cgcgctccac caaggcacgc agccgcacgg tgaggatatt cttcgccagg ccgatgcttt
      901 tttgaaattc gccgaagcgg cgttttccca ggaaggcctc gcggatgatc aacagcgacc
      961 accagtcgcc gagcgcgtcc agcgatcgcg cgatcgggca ttccgccttt tcaaggctgg
     1021 tgcgtttcac catgggcgtc ctttcggttc caaaaaatcg atcccgccga tcacggcgtc
     1081 gtgtagttgc attatgaaac cagatcccct agatagtcca tctagtttaa taatgcaacc
     1141 actggaggcg atcgtgaggc ttgcgaacaa gacggcgctg attacaggcg gcaacagcgg
     1201 catcgggctt gcgacggcgc ggctattcgt ggctgaaggc gcgcgggtcg ccatcaccgg
     1261 acgaaatcag gcgacgctgg aagccgccgc aaaggaactg ggaccgaacg cgctcgcgat
     1321 tgtcgccgac gcgaccgata tcgccgcgac cgaacaggcg attcggcagg ccgtcgaaaa
     1381 attcggcaag ctcgacatcg tcttcgccaa tgctggcatc cccggcaata cgccgatcgg
     1441 aggcaccacg ctggctgcat ttgagcaggt catcagaacc aacatcactt cggtgttctt
     1501 caccgtgcag gcggcggcgc cgcatctgaa cgacggcgcg tcgatcatct tgaacggctc
     1561 ggtcatctcc gtgctcggca atcccggcta ctcggcctat gcggcgagca aggccggggt
     1621 gcgggcgatg gcccgggtga tggcatcgga actctcgccg cgcggcattc gcgtcaatgt
     1681 ggtcgcgccc ggcgccgcgc ggacgccgat ctggaacggt gcggctccca cggccgaagc
     1741 attcgctgtt ctggacaagc gaatttcccg caccattccg cttggacggc tcggcgaggc
     1801 cgaggaagtt gccaagacgg tgttgttcct cgcctccgac gatgcttcga acgttcaagg
     1861 cgcggagatc ttcgtcgatg gcggcgcgac gggttcacct gcgggagcgc cgatctatcg
     1921 cggatagacg caagttccag ccaacatcac tgcatccgag cagcgtgatc tgacgaatag
     1981 tctcacgtcg gttgcgttgt ccggttccat cacctggtgg aggtatttcg cggaattcac
     2041 tgagtgcgtt gaaccttcgt cgcagctgcg acgactgttc cctaggctgg gattttgaac
     2101 cacctacgca ttggagaagc ctgaaatgcc gaaagcctct cgtatgtttg tgccgatttc
     2161 cgcgtcaacc ggttcatccg acagatccga caatagcccg cttgttacga tagcgttgtt
     2221 ttccagcatc ggcctgctgg catccctgat cgccgttctc atgggcgtgc aaggcgtgtg
     2281 gtactaatca cgagctgcat ttcacacaaa tgacatcata aggaagacac cagcccgccg
     2341 tcgccgctcg atggcggcag gcggtgtctc agctcttgct atgccgcgtg gagatcggtg
     2401 gccgcttgca gggcattggc cagcgctttc tcgcggtgtt cgggtccgac ctggattccg
     2461 tcggcggaaa taaattccgg gttcgtgacg ccgataaagc cgaacaccca gcgcaaatag
     2521 gtttccagat gttcgccggc cgccgccggc gtgccggcgc cgtaaaagcc gccgcgcgag
     2581 atcgcgatga taacgcgctt gttgccggcc aatccctccg cgccctgcgc gccatacttg
     2641 aaggtcttgc cggcgaccac gatgcggtcg atccaggcct tgagctggct cggaagcgtg
     2701 aaattgtaca ttggcgcgcc gagcaccacg atgtcggcgg ccagaaactc gtccagcacg
     2761 gcccgaccgg ctgcgaggtc ccgttggagc gcggcctccg gggcggcccc ttgcccggca
     2821 gaaagatgcg agcccgacag gtgcgccaga ggcgtcaagg tcaggtcgcg atagacgatt
     2881 tcgagaccc
//
LOCUS       DASWGM010000421        11210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_723705,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000421 DASWGM010000000
VERSION     DASWGM010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723705"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VGA15_12285"
     CDS             complement(<1..509)
                     /locus_tag="VGA15_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588504.1"
                     /translation="MSLTRRQALASAAAAIASSVVKPAIAAKEPIMIGYLPALTGPSS
                     STGIGINRGIQLAVQEINAAGGIDGRQIELIVRDTQSDPTKAVNGAAELAHREKVSVV
                     FGPVNSGESLAVVPLLARTNTPQLHPCWVDSLTDPKKYPMCFRNAPTNQQIGAAANRY
                     VVDVLKRKRV"
     gene            complement(1053..1499)
                     /locus_tag="VGA15_12290"
     CDS             complement(1053..1499)
                     /locus_tag="VGA15_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007826944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX9588505.1"
                     /translation="MAQLEKEHTTAQNGKVVYTAKTHTSGGRENGVSRSSDGRLDVKL
                     SLPGSARIGTNPEQLFAAGWSACFESAIGLAARKRKIALPADLAIDAEVDLHLAESGY
                     FLSTRLNISLPGIEREVAQSLLDEADQLCPYSKATRGNIDVAINLV"
     gene            complement(1550..2617)
                     /locus_tag="VGA15_12295"
     CDS             complement(1550..2617)
                     /locus_tag="VGA15_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9588506.1"
                     /translation="MDEIKTSETIDRQRRRFFGAAALTLAAAEFGMIGSAGAQAGKAK
                     STGLRTTKPGTHTSFASLKQIDAGVLNVGYGEAGPADGPPVILLHGWPYDIHSYVDVA
                     PLLASAGYRVIVPYLRGYGTTRFQSSDTARNGQQSVVAVDIVALMDALKIAKATIAGF
                     DWGARTANIIAALWPDRCKALVSVSGYLIGNQEAGKMPLPPKAELQWWYQYYFTTERG
                     RVGYDKYRRDFAKLIWQLASPKWDFDDATFDRSAAAFDNPDHVAIVIHNYRWRLGLAE
                     GESKYDDLEKRLAAAPVITVPTITLEGDANGAPHPEPSAYAKMFSGKYSHRTIKGGIG
                     HNLPQEAPQAFAEAVIDVVES"
     gene            complement(2696..3957)
                     /locus_tag="VGA15_12300"
                     /pseudo
     CDS             complement(2696..3957)
                     /locus_tag="VGA15_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
     gene            4320..7163
                     /locus_tag="VGA15_12305"
     CDS             4320..7163
                     /locus_tag="VGA15_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9588507.1"
                     /translation="MTPPALAREVISFGPFSLVTSERPLTREGAPVELSARALDILVA
                     LLSRPNEVVSKNDLLAQVWPDATVEEGSLRFHIVNLRKALARAVHDTSRGYCFVAPIS
                     RSSDRGQVLQGKALAAAVSFPHANLPGRLIGMVGRDDDVLKLSARLNAARFVTIVGSG
                     GVGKTTVAVAVGHHLIEAFSGAVLFVDLSMLNDPVLVATAVASMLGLSVQSADVAPSL
                     IAYLRDKRIFLILDTCEHLIEAVAALASTIFIAAPQVHILATSRETLQVEGEHVYRLD
                     PLACPPDDAGLTAAVAQTFSAPKLFVERAVASGAHLDFSDAEAAIVVAICRKLDGVAL
                     AIELAARRVEAYGLHQTAALLDQRLTLLWVGPRIAPPRQKTLHATLDWSYGLLSDLER
                     QVLRRLAIFVGHFTLDAALAIVTGATLDQAGVFGAIDSLVAKSMVATRPVGAMMRYRL
                     LDTTRAYALEMSIDDTDLADLAVRHATYYRRWLEQAGTEWSTLSTGVERAPHFAGLNN
                     VRAALEWCFGVNDNAEIGVGLAAAAAPVFLTMSLLPECHRWSERAILALDDATRGGSE
                     EMHLQACLGISSMHMLGQSDAARMALDRSLAIAEEHGDVLSQAGLLGMLHMFHFRGGD
                     FNTALHHAKRCRAVAGTIEDPAATALAHSILGRSLHLMGDHSVARVELEASLRNWLRT
                     QRTTIYLAYDRHYRAGVALARTLWLQGYPAQAVERAHEAIRGAERIDHPASLVVVLAW
                     AASVFLWTGDLRSAEEHINACISLAESYSLGPLMAVGRGRQGELAIRRGDASDGFEGL
                     RASLARIHAARYELLTTEFNISLAQGLAAIRRLAEAITLINETIRRVETNGDVLYVPE
                     LLRVKGGLLLSTPRPSVEDAGKCFMQSLELSRRQGARAWELRTATDLAALFANQGQSE
                     RGRALLQPVFEQFVEGWDTADLKAAERVLSNLG"
     gene            complement(7196..9097)
                     /gene="cysN"
                     /locus_tag="VGA15_12310"
     CDS             complement(7196..9097)
                     /gene="cysN"
                     /locus_tag="VGA15_12310"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX9588508.1"
                     /translation="MPTTILDAPVGPPALAETAEKSLLRFLTCGSVDDGKSTLIGRLL
                     YDSKLVLDDQLAAVARASGRYGTLGEETDLALLVDGLEAEREQGITIDVAYRYFATPR
                     RAFIVADTPGHEQYTRNMATGASTADLAVVLVDARKGLLAQSRRHSYICALLGIRSVV
                     LAVNKIDLVAWSEQVFDRIVAEYLDFVRPLNFSRLVPIPMSARYGDNIASRSARTPWY
                     DGPSLLDHLETAEVAQTAARLPLRFPVQWVNRPNLDFRGYAGTLASGRVAVGDNVVVA
                     SSGKVSRVERIVTFNGDRDSAIAGDAVTLTLADEIDIGRGDVLVPPQSRPEVADQFAA
                     HLLWMNEEPLLPGRSYLLRIGTKTLPAQVTMLKHKIDINTLEQRPGRTLGLNEIGFCN
                     LSTASPVAFDAYTDNRRTGAFILVDRFTHATAGAGMIAFGLRRATNIHRQSHLVDKTA
                     RAALKHQTPCVLWFTGLSGSGKSTIANLVEKALHERGIHTMMLDGDNIRHGVNRDLGF
                     TDEDRVENIRRVGETAKLFVEAGLVVLSCFISPFRAERDMVRELLAPGEFAEIFVDVP
                     LEECIRRDPKGLYAKALAGEITNFTGIDSPYEPCQAPDIHVAGVEETPETAAARIVDW
                     FFARQGRRL"
     gene            complement(9097..10140)
                     /gene="cysD"
                     /locus_tag="VGA15_12315"
     CDS             complement(9097..10140)
                     /gene="cysD"
                     /locus_tag="VGA15_12315"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX9588509.1"
                     /translation="MNMHRVEVASRTRAKESAVVEPARSADEIQLASAIRRYAAKASA
                     APRRQLTPHLRRLEAESIEILREVAAEFRKPVMLYSIGKDSGVMLHLARKAFFPASIP
                     FPLLHVDTTWKFREMIAFRDLMARELGLDLLLHVNREGLARGIDPIASGPALHTQVMK
                     TEALKQALDAHGFDAALGGARRDEEGSRAKERIFSFRSVNHVWDPRHQRPELWNLFNT
                     RIHDGESVRVFPLSNWTETDVWSYIAAEAIPVLPLYFARPRPVVRRSGALIVVDDKRL
                     PLELGEIPEMLTVRFRTLGCYPLTGAIESDAADVDDIVVEMQAATRSERQGRLIDHDD
                     AASMERKKREGYF"
     gene            10900..>11210
                     /locus_tag="VGA15_12320"
     CDS             10900..>11210
                     /locus_tag="VGA15_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588510.1"
                     /translation="MATTVFNKTALANGTSTLNVRADALNQSSDLVQAGVVFNDAIRA
                     SLGLFNLSGSGNQNPFLGSYTTDIHAVQNDIAAMLATPAGVTLGGQAFTLNTTDTAVL
                     T"
BASE COUNT         2112 a         3388 c         3515 g         2195 t
ORIGIN      
        1 accctcttgc gcttgagcac gtccaccaca tagcggttcg cagcggcgcc gatctgctgg
       61 ttggtgggtg cgttgcggaa gcacatcgga tatttcttcg ggtcggtcag gctatcgacc
      121 cagcacggat ggagctgcgg cgtgtttgtg cgggcgagca acggcacgac cgcgagcgac
      181 tcgccggaat tgaccgggcc aaagacgacg ctcactttct ctctgtgtgc gagctccgcc
      241 gctccgttca cggccttcgt cggatcgctt tgggtgtcgc gcacgatcaa ctcaatctgc
      301 cggccgtcga tgccgccggc tgcgttgatc tcctgtaccg ccagctggat tccgcgattg
      361 atcccgattc cggtcgatga agagggacca gtgagagctg gaagataacc gatcataatc
      421 ggttcttttg cggcgatcgc aggtttgacg acgctgcttg caatcgcggc ggcggcggac
      481 gccagcgctt gacgacgggt caacgacatt ttgatttcct ccctgagcgt tcttgattca
      541 ctcccaaact tgctgaggtt tctttgacgt cggatttttt cgatgtccgc gacggactat
      601 agcgttggct cgacggttcg catagcgata cccttaaagc tcaacccgga gtttgcgatc
      661 gcgtgatccc gtcatgcggg ccggcctatg atgcagcaca tcatgcgagg ttgatccaat
      721 tccactctcg catcataagg cgtgaactca tgaatccaaa ggtgtctgcg gacgtccgct
      781 tttgatcaag gcacgtcgct ctggctggcc ctttgagcag ggcattccag cgcttgtcgg
      841 ctttggctgc ggacacctcg acgggcggag agcaacgatc gcgccaacac tgagcactgc
      901 gatcgtcgcg gcatcgctgc aaccttcgct ttctcaccgt ggccacgatc cagagcttaa
      961 gcgcaatgat ctcgctagga ccgcaccagt ttcgcaccag aaaggattcc gccaaaccgc
     1021 gagcgttcag ggcgcgcgcg agccggttcg gctcagacca aattgatcgc aacgtcgatg
     1081 ttgccgcggg tggccttcga atacggacaa agctggtccg cttcatccag caggctttgg
     1141 gcaacttcgc gctcgatacc tggcaggctg atgttgaggc gggtgctgag aaagtaaccg
     1201 ctttctgcga ggtgcaggtc cacttcggca tcgatcgcca ggtccgccgg cagagcgatc
     1261 ttcctcttgc gggccgcaag cccaatcgca ctttcgaaac aggctgacca acccgcggcg
     1321 aacagctgct ccggattggt gccgatgcgc gccgagcccg ggagtgaaag cttgacgtcg
     1381 aggcggccat cagagctgcg ggatacgccg ttttctcgcc caccgctggt gtgggtcttg
     1441 gccgtgtaca ctacttttcc gttttgggcc gtcgtgtgtt ccttttcgag ttgggccatg
     1501 gaactctcct gttgcttttc ctgcttctcc ttgcgccagc gacgatcaat caactttcga
     1561 cgacgtcgat gacagcttcg gcgaaggcct gaggggcttc ctggggcaga ttgtgcccga
     1621 tgccgccctt gatggttcgg tgcgaatatt tgcccgagaa catcttggcg taggcgctcg
     1681 gctccgggtg tggcgcaccg ttggcatcgc cttcgagggt gatggtgggc acggtgatga
     1741 ccggcgccgc ggcaagccgc ttttccagat cgtcgtattt cgactcgcct tcggcgaggc
     1801 caagccgcca gcggtaattg tggatcacga tcgcgacatg atcgggatta tcgaaggcgg
     1861 cggcgctgcg atcgaaggtg gcatcatcga agtcccactt cggggaggcg agttgccaga
     1921 tcaacttcgc gaaatcgcgc cgatatttgt cgtagcctac ccggccacgt tcggtcgtga
     1981 aatagtattg ataccaccat tgcagctcag cctttggcgg caagggcatc ttgcccgctt
     2041 cctgattgcc gatcaggtag ccgctcaccg agaccagggc cttgcagcga tccggccaga
     2101 gcgccgcgat aatgttggcc gtgcgcgcgc cccaatcaaa gccggcgatg gtcgccttcg
     2161 cgatcttgag agcgtccatc aaggcgacga tatcgacggc gacgaccgac tgctggccat
     2221 tccgcgcggt atcgctggat tggaagcgcg tggtgccata gccgcgcagg tatggcacaa
     2281 tcacgcggta gcctgccgat gccagcaggg gcgcgacatc gacatagcta tggatgtcgt
     2341 agggccagcc gtgcagaagg atgaccggcg ggccatccgc aggacctgct tcgccgtatc
     2401 cgacattcag aacgccggca tcgatctgct tcagcgaagc gaacgacgtg tgcgtcccgg
     2461 gcttggttgt gcgcagccct gtcgatttgg ccttgccggc ctgggcaccc gcggaaccga
     2521 tcatgccgaa ttctgcggcg gcaagggtga gcgccgcagc gccaaagaaa cggcggcgtt
     2581 gccggtcgat tgtttcggat gtcttgatct cgtccatggt cagcctccgt ctttcgatag
     2641 ggcgtcacgt gtaatggaaa ggcggcgcgc gcaccgcgcg cgcccgcttt tcgtctcaga
     2701 ttgactggcg cagcgatttg aacgcagccc gcatctctgc attgaagagc tccggctgtt
     2761 cccaggcggc gaagtgaccg cctttgtcga gcttgttgta gtaaatgagc ttgggatacg
     2821 cccgctccgc ccaactccgc ggagctgcat agatctcgtc tggaaaggcg ctcacggcga
     2881 ccgggatcgt gacgcccctg acgtcgaaga aacctcctcc cgtcgaaatc tgcgcggtgt
     2941 cccagtagag gcgcgccgag gaaatcgcag tgttcgccaa ccagtagagc gtgacgttgt
     3001 cgaggacgtc gtcccgagtc agcccctccg tctttccatc gaagacgcgt gaaatcatgc
     3061 cgtaactgcg gatgtcgtgg tccagcatcc acgcggcgag gccgaccggc gaatccgcga
     3121 ttccgtaaag cgtctgtggc cggttcgaca tttcttgggc gtaggccagg ccgcgcttgt
     3181 agaaatcatc gagttggtcc cacgcatgtt tttatcggct gagaggccgg cgggttgcgg
     3241 gccgccaacc gaaagagctt ttgaaacatc ggctggaacc gtggccgcca tattggtgtg
     3301 aatgccgagc aatcccggcg gctgctgcag cgccatcacc tccgagacgg cattccccca
     3361 atccccgccc tgcgccacgt accgtgtgta gccgaggcgt tgcatcaacg tcgcccacgc
     3421 tcgagcaatg ctgacggggg tccacccggg cgcagtcggc ttgcccgaaa agccgtggcc
     3481 cggtagcgac gggatcacca gatggaaagc gtccgatgcg ctcgcgccat gtgctgtggg
     3541 attggtcagt ggatcgacaa ttttcagctg ttcgatgatc gagccgggcc acccgtgcgt
     3601 gacgatgagc ggcagcgcat tttcatgttt cgaacgaacg tggatgaaat gagtgtcaag
     3661 cccgtcgatc tcggtcacga attgcggcaa ggcgttcagc ttggcctcga tcttccgcca
     3721 gtcgtagtcc gtctgccaat agcgcgcgag tttctgaatc gtcgcgaact ggacgccctg
     3781 cgattgatcc gtgaccgttt cccgttcagg ccagcgggtc gctgcgatgc gtcggcggag
     3841 atcgacgagc gcttcctctg gaaaactgat gtggaaggga cgaattgcat caccttcggc
     3901 cgcagaggcg ggatgtgccg gaagcaagct cgcagcaccg gcaacagcga tgcccatcgc
     3961 ggcggtgccc aaaagacggc ggcgatcctg gtcgattgct tcggtggtta ctgtcgtgcc
     4021 catcgctgat ctcccttgtt tgacgatcga tcgggtcatc acattgtggg ttgttcgaaa
     4081 gcatccgagg gcattccccg gtttgctgcc cgagacacta acccagcggt cgggaaattg
     4141 cctgctatgg cttgttaggc gctgttagcg attacgagca gcagccatgc gcaagacatg
     4201 gtcttgtaac ttttccgaga gcccgggcgc cgccgttttc gtggcattcc tatagtaggg
     4261 aaccagacat tgacctagaa tgcagccccg gggctgacgg ctacgaagat gacgccatga
     4321 tgacaccgcc ggcgctggcc agagaggtca tatcgttcgg cccgttcagc ctcgtcacga
     4381 gcgaaagacc cctcacaaga gagggcgcgc ccgtggaatt gagcgcgcga gcgctcgaca
     4441 ttttggttgc gctgttgtcc cgtcctaacg aggtcgtcag caagaacgat ctattggctc
     4501 aggtctggcc cgacgccacc gtggaagaag gcagtttgcg gttccacatc gtcaacctgc
     4561 gcaaggcgct ggcaagggcg gtgcacgata catcacgagg ctactgcttc gtggccccga
     4621 tttctcgatc gagcgatcgg ggccaggttc ttcaaggtaa agcgcttgcg gcggccgtca
     4681 gttttccgca cgccaacctg cccggccgcc tgatcggaat ggtcggacgc gacgatgacg
     4741 ttctcaagct gtcggctcgg ctgaacgccg cgcgctttgt cacaatcgtc ggctccggtg
     4801 gcgttggcaa gaccacggtt gcggtcgcgg tcggacatca cctcatcgag gccttttcag
     4861 gcgccgtgct cttcgtcgat ctcagcatgc tgaacgatcc tgttctggta gccacggcgg
     4921 tagcttccat gctggggttg tcagttcaat ccgcggatgt ggcgcccagc ctgatcgcat
     4981 atctgcggga caagcgaatt tttttgatcc tcgatacctg cgagcatctg atcgaggcgg
     5041 tggcagcgct ggcgtcgact atcttcatcg ccgcaccgca ggtccacatc ctggcgacaa
     5101 gtcgcgagac tctccaggtc gagggggaac acgtctacag attggatccg cttgcctgtc
     5161 caccggacga tgcggggctc acggcagcag tggcacagac gttctccgcg ccaaagctgt
     5221 ttgtcgaacg cgcggtggcg agcggcgcgc acctcgactt cagcgacgcg gaagccgcga
     5281 tcgtggtcgc catctgccga aagctcgacg gggtggcact cgcgattgag ctggccgcaa
     5341 gacgggtcga agcctacggt ctgcatcaga ccgccgccct tctcgatcaa cggctgacgc
     5401 tgctgtgggt ggggccgcgc atcgcgccgc cgcgtcaaaa gacgctgcat gccacgttgg
     5461 actggagcta cgggctgctt tccgatctgg aacgccaggt actgcgtcgg ctcgccatat
     5521 tcgttggaca ttttacgctc gatgcggcgc tcgcaatcgt gaccggcgcg accctcgatc
     5581 aagcgggtgt tttcggcgcc atcgacagcc tcgtcgccaa gtcgatggtc gcgacccgcc
     5641 cggtaggggc catgatgcgc taccggctgt tggacactac tcgcgcttac gccctcgaaa
     5701 tgagcattga cgacaccgac ctcgctgact tggccgtgcg ccacgcgact tattaccggc
     5761 ggtggctgga gcaggccggg accgaatggt cgaccttgtc gaccggagtg gaacgggcac
     5821 cccactttgc cggtctcaac aatgtccgcg cggccctgga atggtgcttt ggcgtcaacg
     5881 acaacgccga aatcggcgtt ggacttgccg ccgctgccgc cccggttttt ttgacgatgt
     5941 ctttgctccc tgagtgtcat cgctggtccg agcgagccat tctcgccctc gacgatgcaa
     6001 ctcgcggtgg gtccgaggag atgcatcttc aggcgtgcct gggaatctcc tcgatgcaca
     6061 tgctcggaca aagcgatgcg gcgcgcatgg cattggatag aagccttgcg attgccgaag
     6121 aacacggcga cgtcctcagc caagccgggc tattgggcat gctgcacatg ttccatttcc
     6181 gcggcgggga tttcaacacc gccctgcatc acgcaaagcg ctgccgagcc gttgcgggga
     6241 ccattgagga tcctgccgcc accgcattag cccattccat tttgggaagg tcgctccatc
     6301 ttatgggcga ccatagcgtc gcccgcgtgg agcttgaggc ttcgctacgg aattggttgc
     6361 gcacccagcg gaccacgatc tatcttgcct acgatcgcca ctaccgggcg ggcgtcgccc
     6421 tggcgcggac attgtggttg caaggctatc cggctcaggc ggtggaacgt gcacacgagg
     6481 ccattagggg tgccgaacgt atcgaccatc cggcatcgct agtagttgtt ttggcttggg
     6541 cagcgtcggt attcctctgg accggcgatc ttcgaagcgc cgaagagcat atcaatgcgt
     6601 gtatcagcct tgccgaatcc tattccctgg gacctctcat ggcggtggga cgaggccgcc
     6661 aaggggaact ggccatccgc cggggcgatg caagcgacgg cttcgagggc ttgcgagcgt
     6721 ctctggcgag gatccacgcg gcgcgttacg agttgctcac cacggagttc aacatctcgc
     6781 tcgcccaagg acttgcagcg atccgacggc tcgccgaagc catcacgctg atcaacgaga
     6841 cgatccgacg ggtggagaca aacggagatg tcttgtatgt gccagagtta ctgcgcgtga
     6901 agggaggcct tcttctgtcg acgccgcggc ccagcgttga agatgccgga aagtgcttta
     6961 tgcagtcgct cgaattgagc cgacgtcagg gcgcgcgcgc gtgggaattg cgcaccgcta
     7021 ccgacttggc agctctattt gccaaccaag gacaatccga acgcggccgg gcgctcctgc
     7081 agccggtgtt tgagcagttc gtagagggct gggacacagc ggatctgaaa gccgccgagc
     7141 gcgtgctttc gaacctgggc taaaatggtt cgcttatcat tccggactgg gccgttcaga
     7201 gtcttcgccc ctgccgggcg aagaaccaat cgacgatgcg cgcagcggcc gtttccggtg
     7261 tttcctccac tcccgcgacg tggatatcag gcgcctggca cggctcgtac gggctgtcga
     7321 tgccagtgaa attcgtgatc tcgccagcca aggccttggc atagaggccc ttcggatcgc
     7381 ggcggataca ctcctcgagc ggtacgtcga caaagatctc cgcgaattcg cccggcgcga
     7441 gcagctcacg caccatgtcg cgttcggcgc ggaacggcga aatgaagcaa ctcagcacca
     7501 caagacccgc ttcgacgaat agcttggcgg tctcaccgac gcggcggatg ttttccacgc
     7561 ggtcctcgtc ggtaaagccg agatcgcgat tgacgccgtg gcggatattg tcgccgtcga
     7621 gcatcatggt atggatacca cgctcgtgca gcgctttctc gaccagattg gcgatcgtgg
     7681 actttccgga gccagagagc ccagtgaacc acagcacgca gggcgtctgg tgcttgagcg
     7741 ccgcccgcgc cgttttgtcg acgaggtggc tctggcgatg gatgttggtc gcccggcgaa
     7801 gcccgaacgc gatcatgccg gcgccggcgg ttgcatgggt gaaacggtcg acgagaatga
     7861 aggcgccggt ccggcggtta tccgtgtacg catcgaacgc gaccgggctc gcggtcgaca
     7921 ggttgcagaa gccgatttcg ttgaggccaa gcgtacggcc aggcctttgc tccagcgtat
     7981 tgatatcgat cttgtgcttg agcatggtaa cctgggccgg cagcgtcttg gtgccgatgc
     8041 gcagcaaata ggaacggccc ggcagcaggg gttcctcgtt catccacaaa agatgcgcag
     8101 cgaactggtc cgcgacctcc ggcctgctct gcggcgggac gaggacatcg ccgcgaccga
     8161 tgtcgatctc gtccgcgagc gtgagcgtga ccgcatcgcc ggcgatcgcg ctgtcgcggt
     8221 cgccattgaa ggtgacgatg cgctcaacgc ggctgacctt gcccgaggat gccacgacga
     8281 cgttatcacc gacagcgacg cggcccgatg ccaacgtgcc ggcgtagccg cgaaaatcga
     8341 ggttcggacg gttcacccat tgcaccggga aacgcagcgg gagccgtgcg gcggtctgtg
     8401 cgacctcggc tgtctcgaga tgatcgagca gcgagggacc atcgtaccat ggcgtacgcg
     8461 ccgaccttga cgcaatattg tcgccgtagc gcgcagacat cgggatcggc acgagccggc
     8521 taaaattcag cggcctgacg aagtcgaggt attccgcgac gatacgatcg aagacttgct
     8581 cggaccacgc gaccagatcg atcttgttga ccgcgagcac aaccgagcgg atgccaagca
     8641 acgcgcagat gtagctgtga cgccggctct gggcgagcag gcctttgcgg gcgtcgacca
     8701 gcaccaccgc cagatcggcg gtcgacgcgc cggtcgccat attgcgggta tactgttcgt
     8761 ggcccggcgt gtcggcgacg ataaaggcgc gccgcggcgt agcgaaatag cggtaggcga
     8821 cgtcgatggt gatgccttgc tcacgttcgg cctcgaggcc atcgaccagc agcgcgagat
     8881 ccgtctcctc gccaagcgtg ccgtagcggc cgctggcgcg cgcgacggcc gcgagttgat
     8941 cgtccaacac aagcttggaa tcgtacagca gccggccgat cagggtcgac ttgccgtcat
     9001 cgaccgagcc gcaggttaaa aaacgcagca ggcttttctc cgccgtctcc gccaaagcag
     9061 gcggaccgac tggagcatcc aggatcgtcg tgggcatcag aaataccctt cgcgcttctt
     9121 gcgctccatg gacgcggcgt cgtcgtggtc gatcagccgg ccctgacgct cggacctggt
     9181 agcagcctgc atttcgacga cgatgtcgtc gacgtcggcg gcatcagact cgatggcgcc
     9241 ggtgagcgga tagcagccga gcgtgcggaa gcgaacggtg agcatctccg gtatttcgcc
     9301 aagctcgagc ggcaggcgct tatcgtcgac cacgatcaag gcgccggagc ggcgcaccac
     9361 cggccgcggc ctggcgaaat agagcggcag caccggaata gcctcggccg cgatatagga
     9421 ccacacgtcc gtctcggtcc agtttgacaa cggaaacacc cgaaccgact cgccgtcgtg
     9481 gatccgggtg ttgaacaggt tccacagctc cgggcgctga tggcgcggat cccaaacgtg
     9541 gttgacgctg cggaacgaaa agatccgttc cttggcgcgg ctgccctcct catcgcgccg
     9601 cgcgccgcca agtgctgcat cgaagccgtg agcatcaagc gcctgcttga gcgcctcggt
     9661 tttcatcacc tgggtgtgca acgccgggcc cgaggcgatc ggatcgatgc cgcgggcgag
     9721 cccctcgcgg ttgacgtgga gcagcagatc gaggccaagc tcacgggcca tgaggtctcg
     9781 gaatgcgatc atctcccgga atttccaggt cgtatcgaca tgcagcagcg ggaacggaat
     9841 cgaggcaggg aagaaagcct tgcgtgccag atgcagcatg acgccggaat ccttgccgat
     9901 cgaatagagc atgaccggct tgcggaactc ggccgcgacc tcgcgcaaga tctcgatgga
     9961 ttcggcttcg aggcgacgca aatgcggcgt gagttgacga cggggcgccg cggaggcctt
    10021 tgccgcatag cgacggatgg cggaagcgag ctgaatttcg tccgcgctcc gagcgggctc
    10081 gacgacggca gattcttttg cccgggttct ggaagccacc tcaacgcgat gcatgttcac
    10141 gatcgtgtac tctgtaaaac gcggcggacg acgcacagca agccgaaaca cgacagacag
    10201 gtatcgactg gatcccgaca ggcgtgatcg cggtcaaact tgacctaaac atgcgtcgtt
    10261 tcggacggaa ctgtgactat gttctgacca aacgaggcga tcaggtcgcg cgaacagcgg
    10321 catggagcgg aatgtcagtg tcgctaaggc tggtcgacgc tctgtgcaca tgaagcgcac
    10381 aggcgcggcg accatcacca gactgaaatc aggctcgtga agagtccagg tccgacgaaa
    10441 gagaaagaga aagtatgtcc atgagacatt cctccgccgg aaggacgcag agactcgcca
    10501 acatcaggac aatgcgatga gctagttgtt ggacgcgccg gctaactgcc tatgaggccc
    10561 ggcaaaagca gtcccgccga gagttctgcg acgcgcattc agagctttcc atcggtcaat
    10621 tcggatcagc cgaggcgaca ctggcagccg gtccatgccg cggtttgcgc cacctgatcg
    10681 cctcgtttgg tcggaacata atcacagttc cgtccgaaac gacgcatgtt taggtcaagt
    10741 ttgcctccga tcaatcggtt gcgcggcgca taacaaccgc tcaccagctc aacatgccgg
    10801 cgaacgtcgg ttaaggggcc cttcagtttt gaacatcctc gtaatcctgt gggacgcgtc
    10861 aggcaggaga cgagcgatga aatccgggga gacgatctaa tggcaaccac cgtgttcaac
    10921 aagaccgcac tggcgaacgg tacgtcgact ttgaatgtgc gcgcagacgc gctcaatcaa
    10981 tcgagcgacc tggtgcaagc cggcgttgtc ttcaacgatg ccatccgtgc gtcgctgggc
    11041 ctgttcaatc tgtcgggcag cggcaaccag aaccccttcc tgggcagtta cacgaccgat
    11101 atccatgcgg tccagaacga catcgccgcg atgctcgcca ctcccgctgg cgtgacgctc
    11161 ggcggtcagg cctttaccct gaacaccacc gacaccgcgg tgttgaccaa
//
LOCUS       DASWGM010000422         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_307012,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000422 DASWGM010000000
VERSION     DASWGM010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307012"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VGA15_12325"
     CDS             <1..572
                     /locus_tag="VGA15_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX9588511.1"
                     /translation="SDPAKLAERARAQRHEILLQVPMEPFDYPDNDPGPQTLLTTLGA
                     EQNIDRLYWHLSRFQGYAGIANFMGARFVVTDAAMQPIIREAAKRGLGFLDDGLAPRS
                     VAATLATSQGMPFVKADFTIDAVPTLAEIDRALAKLENLAKEHGTAVGVASALPISIE
                     RIGVWIKALESHGIMLVPLTTAMLKSKSG"
     gene            647..1150
                     /locus_tag="VGA15_12330"
     CDS             647..1150
                     /locus_tag="VGA15_12330"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX9588512.1"
                     /translation="MARYEDLPYRTCVGMMLINAAGLVFIGRRAGGIEHVDQSHVWQM
                     PQGGVDPGEDTWSAAKRELYEETSVRSVEKLGEVAEWLIYDIPRTVAGRTWKGRYRGQ
                     RQKWFAVRFTGEDAEINVASPGDGHKAEFVSWRWEPMKNLPNLIVPFKRPVYERVVKE
                     FSKLAGS"
     gene            1157..1654
                     /locus_tag="VGA15_12335"
     CDS             1157..1654
                     /locus_tag="VGA15_12335"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX9588513.1"
                     /translation="MLDSQPYRPNVGIALFNASGNVLIGRRFRDDGPEIILPGLEWQM
                     PQGGIDANEDPREAVMRELWEETGVVHANYLDETDWLTYEFPPFLVPSSHRLAKFRGQ
                     RQKWFALRFTGADDEIDPLTPRNGQPAEFDQWRWERLDRVADLVVPFRRDVYRTVARR
                     FAEFA"
BASE COUNT          349 a          551 c          568 g          341 t
ORIGIN      
        1 gttcggatcc cgccaaactg gccgagcgcg cgcgcgcgca gcgtcacgag attctattgc
       61 aggttccgat ggagccgttc gattatcccg acaacgaccc cggcccgcaa accctgctca
      121 cgacacttgg cgccgaacag aacatcgacc ggctgtactg gcacctcagc cggttccagg
      181 gctatgccgg gatcgccaat ttcatgggcg cgcgcttcgt tgttaccgac gcggccatgc
      241 agcccatcat ccgcgaggcc gccaagcgcg ggctcggttt cctcgacgac ggcttggcgc
      301 cacgcagcgt cgccgccaca ttggcgacca gccaggggat gcccttcgtc aaggccgatt
      361 tcaccatcga tgcggtcccg acattagcgg aaatcgaccg ggcgctcgcg aagcttgaaa
      421 atctggcgaa agaacacgga accgccgtcg gcgtcgcctc ggcgctaccg atctcgatcg
      481 agcgaattgg cgtctggatc aaggcgcttg agagccatgg catcatgctt gtgccattga
      541 caaccgcaat gctgaaatca aaatcgggct agggcaactt ttgggctggc gagactttcg
      601 gatccaccga tgctcgccca tatggaccgc aggaggcttt cacggaatgg cgcgctacga
      661 agacctgccc tatcgaacat gcgtcggcat gatgctgatc aacgcagccg ggctggtctt
      721 catcggtcgc cgcgccggcg gcatcgaaca tgtcgatcaa tctcacgtct ggcagatgcc
      781 gcaaggcggc gtcgatcccg gtgaggatac ctggtctgcc gcgaagcgcg aattgtatga
      841 ggagaccagc gtccgctcgg tcgagaaact cggcgaggtc gcggaatggc tgatctacga
      901 cattccgcgc accgtggccg ggcgcacctg gaagggccgc tatcgtggcc agcggcaaaa
      961 atggtttgcg gttcgtttca ccggcgagga cgccgagatc aatgttgcca gccccggcga
     1021 cggccacaag gcggaattcg tcagctggcg ctgggagccg atgaagaatc tccccaacct
     1081 gatcgttccg ttcaagcgcc cggtctatga gcgcgtggtg aaggaatttt cgaagctcgc
     1141 gggcagctag ctcgcgatgc tcgacagcca accctatcgt cccaatgtgg ggatcgcgct
     1201 gttcaacgct tccggcaacg tgctgatcgg gcggcgcttt cgggacgacg gaccggaaat
     1261 cattcttcca ggccttgaat ggcagatgcc gcagggcggc atcgacgcca acgaggatcc
     1321 gcgcgaggcg gtgatgcgcg aactctggga agagaccggc gtggtgcacg ccaattatct
     1381 cgacgagacc gattggctga catacgagtt tccgccgttt ctcgtgccgt cgtcacaccg
     1441 gctcgcaaaa ttccgcggcc agcggcagaa atggttcgcg cttcggttca ccggggccga
     1501 tgacgaaatc gatccgctga cgccacgcaa cggccaaccg gcggaattcg accagtggcg
     1561 atgggaacgg ctcgatcgcg ttgccgatct cgtggtgccg ttccggcgcg atgtgtaccg
     1621 gacggtggcg cggcgtttcg ccgaatttgc gtgagttact ctccctcccc ccgcttgcgc
     1681 agggctgtcc cgggaaaaat cacacatagt tgaaccccag ctgatcggga gagaagttgg
     1741 gctttggttt gctcggattg actggtggcg gggtgtcgat ttctgcgatc gattttcgca
     1801 tgaacaagc
//
LOCUS       DASWGM010000423         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2663229,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000423 DASWGM010000000
VERSION     DASWGM010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2663229"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VGA15_12340"
     CDS             <1..1195
                     /locus_tag="VGA15_12340"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9588514.1"
                     /translation="NGASTLEGYTPDTDATIVTRMLDAGGTIVGKVHCEYFCFSGGSH
                     TCAAGPVHNPHKMGYSAGGSSSGSAVVVATGEADMAIGGDQGGSIRIPASFSGIYGMK
                     GTYGLVPYTGVMPIELTIDHTGPMTRNVRDNALLLEVLAGADGLDPRQGAPKVAKYTE
                     ALDGGVRGMRIGIVKEGFGLPVSEPDVDAKVMAGAQLFRKLGATVDEISVPMHLLAPA
                     IWLPIAAEGATEFMMKGNGMGTNWRGLYNTTLLDAHSAWHHRADELSDSLKITMLVGQ
                     YFTKHYRGHFYAKAQNLNRKLRAAYDAALSNYDLLLMPTLPMKATPIPPPDAPRDLYI
                     QRAFEMVPNTAPFNASGHPAMSIPCGMSDGLPIGLMLIGKYYDELAIYRAAAAFESAG
                     DWTKM"
     gene            1254..2009
                     /locus_tag="VGA15_12345"
     CDS             1254..2009
                     /locus_tag="VGA15_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9588515.1"
                     /translation="MDLGLKGKSVLVTGGSKGIGLAIAELFADEGANVAICARNADEV
                     GNVVKALAARRVKSWGGAIDVADPAALKKWVEDAAGELGGVDTMVCNVSALAVGDTAE
                     TWEKSFRTDMMHTVNSVAAAVPYLEKSKSASIAIISSVSGFEVDFAAGSYGAFKAALI
                     HYAKGLSNQLIGKGIRVNAISPGNTYFEGGIWQNIENGMPDLYKMAMSLNPTGRMGTP
                     QEVAAGVVFLASPLASRISGTNLIIDGALTKAV"
     gene            2073..>3215
                     /locus_tag="VGA15_12350"
     CDS             2073..>3215
                     /locus_tag="VGA15_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9588516.1"
                     /translation="MSNNIGRRDFLGTVALVGAAAAAGRASLINSAEAATASSDGITS
                     ASASELAEAVRSKKWSCKEIVEAHLDRIAKVNPKLNAVVQLTAEAARKEADEADAALA
                     RGEIKGPLHGVPVTIKDTLETAGVICTGGTKGRANYVPKADATAVARLRAAGGIILGK
                     TNVPELAGAAETDNLVYGRTNNPYDLARTPGGSSGGEAAIIAAGGSPLGLGTDAGGSI
                     RIPAHYCGLAAIKPTSGRVPRTGQFPMPLGARNPVFHVSLIARKVGDLALALPIVAGP
                     DFRDHSIVGMPLGEPAKVVLRDLKVAYFDDDGVATPTKEIVAAVRDSAKAFTDAGIKV
                     EKSRPPDAAKPGGVYFDISRGDGGAGTRGFLKSIGTNEISPMFERAL"
BASE COUNT          646 a          975 c         1024 g          570 t
ORIGIN      
        1 gaacggtgcc tcgacgctcg agggttacac gcccgacacc gacgcgacca tcgtcacccg
       61 catgctcgat gccggcggca ccatcgtcgg caaggtccat tgcgaatatt tctgcttctc
      121 cggcggcagc cacacctgcg cggctggtcc cgtgcacaat ccgcacaaaa tgggctattc
      181 ggccggcggg tcgtcctcgg ggagcgccgt cgtcgtggcg accggcgaag ccgatatggc
      241 gatcggcggc gatcaaggtg gatcgatccg cattcctgct tccttcagcg gcatctacgg
      301 gatgaagggc acctatggcc tggtgccata taccggcgtg atgccgatcg agcttaccat
      361 cgatcacacc ggcccgatga cccgcaacgt gcgtgacaat gccctgctgc ttgaggtgct
      421 cgcaggagcg gacgggctcg atccccgaca aggtgcgccg aaggttgcaa aatataccga
      481 agcgctcgac ggcggcgtca ggggaatgcg catcggcatc gtcaaggaag gctttgggct
      541 tccggtctcc gagccggacg tcgatgccaa ggtcatggcc ggcgcccagc ttttcaggaa
      601 actcggcgcc accgtcgatg aaatatcggt gccgatgcat ctgctggcgc ccgccatctg
      661 gcttccgatc gccgcggagg gcgccaccga attcatgatg aagggcaatg gcatggggac
      721 caactggcgc ggtctttata acaccacgct gctggatgct cattccgcct ggcaccatcg
      781 cgcggatgag ctgtccgaca gcctcaagat aacgatgctg gtcggtcaat atttcaccaa
      841 gcactatcgc ggtcatttct atgccaaggc gcagaacctg aatcgaaaac tgcgcgccgc
      901 ctacgatgcc gcgctgagca attacgattt gttgctgatg ccgacactgc caatgaaggc
      961 gacgccgatc ccaccgcccg atgcgccgcg cgatctctat atccagcgcg cattcgaaat
     1021 ggtccccaac acggcgccgt tcaacgcatc gggtcatccg gcgatgagca ttccctgcgg
     1081 gatgagcgac ggactgccga tcgggctgat gctgatcggc aaatactacg atgagttggc
     1141 gatctatcga gccgcggcgg cattcgagag cgccggcgac tggaccaaga tgtaagtcaa
     1201 cgacgcggcc tgctgcgcga cagcaggccg cggcatcatc aacggaggcg acgatggatc
     1261 ttggattgaa gggcaagagc gtgctggtga ccgggggaag caagggcatc gggctggcga
     1321 tcgccgaact gtttgcggat gaaggcgcca acgttgcgat ctgtgcgcgc aacgccgacg
     1381 aggtcggcaa cgtcgtcaag gcgctggcgg cgaggagggt caagtcatgg ggtggagcaa
     1441 tcgatgtcgc tgatcctgct gcgttaaaga aatgggtcga ggatgcagcc ggtgaactcg
     1501 gcggtgtcga caccatggtt tgtaatgtca gcgcgcttgc cgtcggcgat acggcagaga
     1561 cgtgggaaaa gtcattccgt accgacatga tgcataccgt caattccgtt gcggcggccg
     1621 tgccttacct ggagaaatcc aaatccgcct cgatcgcgat tatctcgagc gtttcaggtt
     1681 ttgaggtcga ttttgctgcc ggttcctacg gcgccttcaa ggcggcgctg attcattacg
     1741 ccaaagggct cagcaaccag ctcatcggca agggcatccg cgtcaacgcg atttcgcccg
     1801 gcaataccta tttcgagggc ggcatatggc agaacatcga gaacggtatg cccgatctct
     1861 acaagatggc gatgtcgttg aacccgaccg gacgcatggg cacgccgcag gaagtcgccg
     1921 ccggcgtggt gttcctggca agcccgttgg cgagccggat ctccggcacc aacctcatta
     1981 tcgatggcgc gctcaccaag gccgtctagc gaagccggct ggttgccgga aatgccgacg
     2041 ctgtcctcga accaaaacgg atcggattgc aaatgagcaa caacatcgga cgccgggatt
     2101 ttctgggtac ggtcgcgctc gtgggcgcag cggcggcggc cggacgagcc tcgttgatca
     2161 attccgccga agctgccacg gcgtcgagcg acggtatcac ctcggcatcg gccagtgagc
     2221 ttgctgaagc tgttcgatcg aagaaatgga gctgcaaaga gatcgtcgag gcgcatctgg
     2281 accggatcgc caaggtcaac cccaaactca atgcggtcgt tcaactgacc gcggaggcgg
     2341 cacggaaaga ggcggatgaa gccgacgcgg cgctcgcccg cggcgaaatc aagggcccgc
     2401 ttcacggcgt ccccgtcacc atcaaggata cgctggaaac cgccggtgtg atctgcaccg
     2461 gcggcaccaa gggcagagcc aattacgtgc ccaaggcgga tgccaccgcg gtcgcgcggc
     2521 tgcgtgcagc cggtggcatc atccttggca agaccaatgt gcccgaactc gcaggcgcgg
     2581 ccgaaactga caatctggtc tacgggcgga ccaataatcc ctacgacctc gcgcgcacgc
     2641 cgggcggcag cagcggtggc gaggccgcga ttattgcagc cggcggctcg ccgcttggtc
     2701 tcggcaccga tgccggtggc agcatccgca tccctgcgca ttattgcggg ctcgcggcca
     2761 tcaagccgac ctcgggccgc gtgccgcgga ccgggcaatt tccgatgccc ctgggcgcgc
     2821 gcaacccggt atttcacgtc agcctgatcg cgcgaaaggt cggggacctg gccctggcgt
     2881 tgccgattgt tgcgggtccc gatttccgcg atcactcgat cgtgggaatg ccgcttggcg
     2941 agcctgcgaa ggtggtgtta cgcgatttga aagtggccta tttcgacgac gatggcgtcg
     3001 cgacaccgac aaaggaaatt gtcgccgccg tgcgcgattc cgccaaggca ttcaccgatg
     3061 ccgggatcaa ggtcgaaaaa agccgaccgc ccgacgcggc aaaacctggt ggcgtctatt
     3121 tcgatatcag ccgcggcgat ggcggtgccg gcacgcgtgg attcctcaag agcatcggaa
     3181 ccaacgaaat ctcgccgatg ttcgaaagag cgctg
//
LOCUS       DASWGM010000424         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2540449,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000424 DASWGM010000000
VERSION     DASWGM010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540449"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1377
                     /locus_tag="VGA15_12355"
     CDS             331..1377
                     /locus_tag="VGA15_12355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9588517.1"
                     /translation="MSAGGAMQFGIFDQNDRGPSPLAEQYENRLKLIEFYDQTGFRAY
                     HMSEHHSTPLNLTPSPSVFLAAAAQRTRRLRLGALVYILPAHHPLRLAEEICMLDHLS
                     RGRLEVGVGRGASPHELRYFGIDPETAPAMYVEAYTVIKQALTQKQVNFNGKYYNFEN
                     VPVEIKPAQLPHPPFWYAVPVPEGAVWPAQNSINIVCAGPVPKVREITDRYRAEWAAA
                     GNSPDDIPLLGINRFAIAADTDREAMELGRRAWPAFYASFMKLWKKHGTQPRYARIPE
                     DFETMVGNGGALAGSPGTIRDQVRNMADEAGASYFIAQFSFGDLSHQEVMHSAGIFAR
                     EVLPASRKHVARAV"
     gene            1567..1959
                     /locus_tag="VGA15_12360"
     CDS             1567..1959
                     /locus_tag="VGA15_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588518.1"
                     /translation="MRTILGVILITAATISMASAQSFSMDHGHAADAARAARAAANAA
                     ADKAAAIAAGKPLTPSQSTDFSVNGSGVIHAAPPRTPEEQKADAEAQAAWQARCRPTV
                     IEDREGMRRTKYAESNCDLSRFNTAGAQ"
     gene            2276..>2655
                     /locus_tag="VGA15_12365"
     CDS             2276..>2655
                     /locus_tag="VGA15_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9588519.1"
                     /translation="MLTISHAAGANEPVVRDITLGELLRWAAETTPDRMALIAGIPDR
                     DNRRQWTYAELYAQALRTAGALRAHFEPGERVAIWAHNIPEWIMVEFGAAMAGVILVT
                     VNPGLRSGEVEYVLKQSRAAGVMVV"
BASE COUNT          533 a          839 c          797 g          486 t
ORIGIN      
        1 tccacggcgg ggtcaaccgg cgcggggctg gaatcgtcgg tgacgagtcc agccttcacg
       61 attggagccg caagccgttg cgaaaactcg gcttcggcgt cgccaacgca agcacgcgtc
      121 gacgtcagga gtaaaagaca aagcgcgaga tgttttgtca tcacccacca tgcaccaact
      181 gaccgcggac cccatgctag ctagcgatgc acggtcgagg gatcaagcac gatccgtgtt
      241 tttggcgatt tgacggcaga cctgcatggc tgatatcgcc aaaatacgtt cccagtccgc
      301 attgccacct cgtcacggct gatccaaaat atgtcagcgg gtggtgcgat gcagttcggc
      361 atattcgatc aaaacgaccg cgggccttca ccgcttgccg aacagtacga aaaccgtctg
      421 aagctgatcg agttctacga ccagaccgga tttcgggcct atcatatgag cgagcatcat
      481 tcgacgccgc tcaatctcac accatcgccc agcgtatttc tcgctgccgc ggcgcagcgt
      541 acgcggcgtc tccgcctggg cgcgctggtt tatatcctgc ccgcccatca cccgctgcgg
      601 ctcgccgaag agatctgcat gctcgaccat ctcagccgag gccggctcga ggtcggcgtc
      661 ggccgcggcg cctctccgca cgagttgcgg tatttcggca tcgatccgga gactgcgccg
      721 gcgatgtatg tcgaggctta caccgtcatc aagcaggcgc tgacccagaa gcaggtcaat
      781 ttcaacggca aatactacaa tttcgaaaac gtaccagtcg aaatcaaacc ggcgcagctg
      841 ccgcacccgc cattctggta tgcggtgccg gtgccggagg gcgcggtgtg gccggcgcaa
      901 aacagcatca acatcgtgtg cgccgggccg gtgccaaaag ttcgcgagat caccgatcgc
      961 tatcgcgccg aatgggcggc cgcgggcaat tcgcccgatg atatcccctt gctcggcatc
     1021 aaccgcttcg cgatcgcggc cgacaccgat cgcgaagcga tggaactcgg ccgccgcgcc
     1081 tggcctgcgt tttatgcaag cttcatgaag ctctggaaaa agcacggcac gcagccgcgc
     1141 tatgccagga ttccggaaga ttttgaaacc atggtcggga atggcggcgc gctcgcaggc
     1201 tctcccggca cgatccgcga ccaagtgaga aacatggccg atgaggcggg cgcgagctat
     1261 ttcatcgcac agttttcctt cggcgatctg tcacatcaag aggtgatgca ctccgcgggc
     1321 atctttgccc gcgaagtgct tccggcgtcg cggaaacatg tcgcccgcgc ggtttgaatg
     1381 acggcgacag catgcggtcc cgcaagacgc ttcaggcgat cgctaaattc caacgcactg
     1441 tgttgcgcgg gcattaacta gcctgcttca cgtgccctca acaactttca aatagcaaca
     1501 tgcggggcga tagcacctgt ccgaacccgc ttgcaattcg ttgcagttct tggaggagtt
     1561 ttagaagtgc gcaccattct tggcgtaatc ctcattaccg ccgccaccat ctcgatggcg
     1621 tccgcccaat cattttcgat ggatcatggg cacgccgctg acgcggcgcg agcggcgagg
     1681 gcggctgcca acgcggcggc agacaaggcg gcggcgatcg ccgccgggaa accgctcacc
     1741 cccagccaat ccaccgattt cagcgtcaac ggcagcggcg tcattcacgc cgcgccgccg
     1801 cgaacgccgg aagaacagaa ggccgacgcg gaagcacagg ccgcctggca ggcgcgctgc
     1861 cgtccgaccg tcattgaaga tcgtgaggga atgcgccgca cgaaatatgc cgaatccaat
     1921 tgcgaccttt cccgcttcaa taccgccggg gcacaataag caaggccaat aaccgacatc
     1981 gtcgccagcg gcttgatcgc gtcccggcac caccggtgcc gggtgacgcc attcgaaatt
     2041 cataagacgc gcatcgtcat cccggctcga ggcggccggc gtttagccgc attcgcagat
     2101 cgcgataccg gccgtcctta tggggcggcc tttatcttcg tcggcgcatt ccgccgaccg
     2161 gggattctcg ccgcgttgca aggctcttga tccatactcc gtaacattcg gcacgcgcgt
     2221 ctcattgaaa ttggctacgc tgtcaaagcg agcccattcg gtggaggaaa cgatcatgct
     2281 gaccatctct catgctgcgg gtgcgaacga accggtggtt cgcgacatca cgctgggcga
     2341 gctgctgcga tgggcggcgg aaaccacacc cgaccggatg gcgctgattg ccgggattcc
     2401 cgaccgggac aatcgccggc aatggactta cgcggagttg tacgcgcagg cgttgcgaac
     2461 cgcaggagcg ctgcgggcgc actttgagcc gggcgaacgc gtcgcgatct gggcccacaa
     2521 cattcccgag tggatcatgg tggagtttgg cgccgccatg gcgggcgtca ttctggtgac
     2581 ggtcaacccc ggcctccgat cgggcgaggt cgaatatgtc ctgaagcagt cgcgcgcggc
     2641 cggcgtcatg gtggt
//
LOCUS       DASWGM010000425         9339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2540473,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000425 DASWGM010000000
VERSION     DASWGM010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9339
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540473"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VGA15_12370"
     CDS             <1..338
                     /locus_tag="VGA15_12370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9588520.1"
                     /translation="PPAPPQPWTDLAKVLELAKRPNAVIKVSGACTLSREPYPFPDIW
                     DPLARVFDAWGFDRCLWGTDWTRAFAVVNYEQAVEPFLKTGRLSDTERAMLMGGACAK
                     AYGWSPKKG"
     gene            449..1525
                     /locus_tag="VGA15_12375"
     CDS             449..1525
                     /locus_tag="VGA15_12375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9588521.1"
                     /translation="MSVTPRMSVHVKGFGHRRRVGECPLSGEDQTSNLNASIKNATLD
                     PPKRAMPMKAAFIERHGGPEVLKFGEMPNPMATSGEVVVDIVAASVNGADWKVREGKS
                     NPLSRFPHILGRDFSGVVSAVGKGVRDLRVGDEVFGVCDVGQEGAYAEKIAIKAAIVA
                     KKTDRLSHVDAAALALAGLTAICAIEDTLKVKAGETILIQGGAGGVASFAVQLAKHLG
                     ARVITTASASNHDYLRKIGADEIIDYNAVDFTKVVRDCDAAFDTVGGDVAQRSFTVLR
                     AGGRAAFIASGAQAPKPDRSDVVALRPSVGRDRQHLERIVALVALGAVRPLEITCYQL
                     SEAVAAHKVSESRHFRGKLVFQVR"
     gene            complement(1791..2171)
                     /locus_tag="VGA15_12380"
     CDS             complement(1791..2171)
                     /locus_tag="VGA15_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008557256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9588522.1"
                     /translation="MSDSSLFEKGLTVRKEVLGEDYVNKSIAGADEFTRTMAEWSTEF
                     CWGALWTRPGLDRRTRSIVNLAMLGALNRPHELKLHVKGALKNGLTKDEIKEILLQVA
                     VYCGIPSGIDAFRNAREAFEEVEG"
     gene            complement(2321..2773)
                     /locus_tag="VGA15_12385"
     CDS             complement(2321..2773)
                     /locus_tag="VGA15_12385"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9588523.1"
                     /translation="MRQDEGQGAGATLANFAAATSWADVAAQSHEAKRSILNFFATAL
                     GSAYDPAITAALRTLSPFSGAATSAIVGRPERLDALGAAFVNAISANLLDFDDTHLDT
                     IIHPAAPVAALCCFSTRDRGAARLGARRGKAARLFAAQRNRMECKHHY"
     gene            2960..3853
                     /locus_tag="VGA15_12390"
     CDS             2960..3853
                     /locus_tag="VGA15_12390"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9588524.1"
                     /translation="MAVETIGFVGTGRMGGPMAERLLDAGYSLCVYDTQSEATKPLVA
                     RGACLAKSPAEVASAADIVLTSLPTPDIVKAVALGPDGIIAGNRASIVIDLSTTGPSA
                     AKLIAEGLKPRSLTLVDAPVSGGIKGAVNGTLAVMVSCPKATYETVQPILKHFGKLFY
                     IGDKPGTAQTAKLANNLMAAAALVITSEAVAMGVKGGVDAKLLIDIINASSGRNSASE
                     DKFPRAVLPGTFDFGFSTGLSYKDVRLCIDEAEAMGVPMVCGSIVRQMLAITNAKYGA
                     SSDFTSIAKVLEEWSGVEMRG"
     gene            3887..5275
                     /locus_tag="VGA15_12395"
     CDS             3887..5275
                     /locus_tag="VGA15_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9588525.1"
                     /translation="MTIGSTGPSVVSLARQLARESLAVDLGRFGADVVAKAKNCLLDF
                     LSCAFEARNHPWSRQAIGIARGIENGATIIGTRKLSTPGDAAFANATLGHGLVREDMH
                     AASICHHGVVLWPTLLALSERTRTPGTTLLAAAIVGYEAGAQIGRALFTADLARLFRP
                     TGLVAPLGAALAGSRALGLTEDAATSAVSIAANTSSGLNEWPRAGGSEMYFHPGFAAR
                     NAIAAIELAEAGACASETILEGEAGLFAAYRRQPAPVGIRLFAGQPEIMAVYNKPAPA
                     CNFAQTAAQAALRTARELKRSEDIETVSIRVPEAAARYPGCDSKGPFQNALQAKMSIP
                     FSVAAVLARGALEEDNYAHIGHPQILRLVERTDLQSEASLTAAFPANQGAEVLVRLRD
                     GNTICQRLDNVIAATPQEIRARFRRAAVDVVGDKCARHLEELVDRCESLSDSRVIAAL
                     CRLEPSERRPAS"
     gene            5388..6335
                     /locus_tag="VGA15_12400"
     CDS             5388..6335
                     /locus_tag="VGA15_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588526.1"
                     /translation="MSQALEGFLQALTAGLLIGAVYGLMCVGLGLIFGVMRIINFAQG
                     DFMMLGMYAAFYLFTALGIQTTFGNAIGPFVAILLAGPVLAVFGYAIHLALISRVSGT
                     RTSSLEGEGHYAQLILTLGIALVLQNGGLIVFGSVLASIRTPLSSSAWELGPFFDISV
                     FVNKARGIDAVVSFATMILLTLLITRSRIGKSMRAAADNPTAATYMGIDVDRAHRVAF
                     ALGTGITAIAGGLLATNYPFHPFVGVEYVIVMYAGVVLGGMGSIIGAFWGGMTIGLVQ
                     QMSTLVLPTQLQNAAIFAVFLLIIFFRPQGFFGRIVERT"
     gene            6338..8122
                     /locus_tag="VGA15_12405"
     CDS             6338..8122
                     /locus_tag="VGA15_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9588527.1"
                     /translation="MRGLRPLLPVLVFTALYAALSLSVTNSYYQLVMTLVPVWAVFGL
                     SWNLLSGYTGLISFGHAAFFGIGAYVTALGQIYFDLSPWVLIPVAATLGGVAGLLIGF
                     PTFRLQGHYFALAMLAYPLAILYVFEWLGFQEVTLPIKRDNPIAYMQFADPRLYTLLA
                     VAMMLGTILLTRIIENSRFGMALLAIKQNEAAAEAAGIDTLAWKLRAIGLSGAIAGAI
                     GGFYAVVLLVVTPQSVFGMLVSAQALTVAMFGGVGTVWGPVIGSVILIPLAETLNAEA
                     GSRLPGIQGVIFGLAIISVILVAPEGLFWKFRDFLRRRSAPQAAAPSSTPDPRLADAV
                     PSAPQRSRSERTQATGDVVLEVRNLSRSFGGLKAVQNVSFKLRQNEILGIIGPNGAGK
                     TTVFNLMNGFLRPGTGEILINGRDMSGRKPHELCEAGVGRTFQVMRPFLRMSISDNVV
                     VGAYVRAGTDAEARKLAADAIARVGLSHIADRIAGELTTKELRLMELARALAGQPRIL
                     LLDETLAGLGHDEADEVVAVIQRLARDGMTIAIIEHTMQAMVRLVDSFLVLDHGAVIV
                     EGEPEAVTRDSRVIEAYLGKKWMAHAPH"
     gene            8109..8816
                     /locus_tag="VGA15_12410"
     CDS             8109..8816
                     /locus_tag="VGA15_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588528.1"
                     /translation="MLRIEGLTAGYSAIPVLNGVSIKVERGQFVAIVGPNGAGKTTLF
                     KTISGIVRPSSGAITFEDTDLLSVRPARRAHLGIAHVPEGRQVFPSLTVMENLEMGAM
                     TDAGRRDWKRNIERIFEWLPVLAERRGQFAGTLSGGQQQMLAIGRGLASSPKLLMLDE
                     PSMGLAPAIADFIFERLIEIRRQSNLTILLVEQRVAEALESADHGYVLEAGRVALEGN
                     NQTLRADDRVRRAYLGM"
     gene            8854..>9339
                     /locus_tag="VGA15_12415"
     CDS             8854..>9339
                     /locus_tag="VGA15_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588529.1"
                     /translation="MSQQKITDRTSRTSVTRRTVLSGAAAMGLSTAARAQQPAEVKVG
                     LIVPLSGIYTRPGQVMRMGAEMGIEHINAQGGIKSLGGAKLKLVVIDCGDTTEKAKNA
                     AQRMVAQETDLVAATGAYLSSFTLAVTEVTERAELPMLTLSYSDLLTERGFKFIFQTA
                     AP"
BASE COUNT         1764 a         2872 c         2862 g         1841 t
ORIGIN      
        1 tgccgcccgc gccgccgcag ccctggaccg accttgcgaa ggtgctggaa ctcgccaagc
       61 gcccgaacgc ggtgatcaag gtcagcggcg cctgcacgct gtcccgggag ccgtatcctt
      121 tcccggacat ttgggatccg cttgcccgcg tgtttgatgc gtggggtttc gatcgctgct
      181 tgtggggcac ggactggacg cgcgcgttcg cagtcgtcaa ctacgagcag gccgtcgagc
      241 ccttccttaa gaccggccgc ctgagcgaca ctgagcgggc aatgctgatg ggcggtgcct
      301 gcgccaaggc ttatggctgg tcaccgaaga aaggttagca ccggctaata gaggtcgcct
      361 tcgccatgct ggcaatgcct gaccgcagaa gctaagcaag tgctgggcac cgaaccacgc
      421 aagccgccgc agggcggctt actttcatat gagcgtcacg cctcgcatgt ctgttcatgt
      481 gaagggtttc ggacatcgcc gtcgagtcgg tgaatgtccg ctttcggggg aagaccagac
      541 atcgaatttg aatgcttcaa tcaaaaatgc gaccttggat ccgccaaagc gagcaatgcc
      601 aatgaaagct gccttcatcg aacgacacgg cgggccggaa gttctgaaat tcggcgagat
      661 gcctaatccg atggccacat ccggcgaggt cgtggttgat attgtcgccg ccagcgtcaa
      721 tggcgcggac tggaaggtgc gtgagggaaa atcgaacccg ctttcgcgat tccctcacat
      781 tcttggacgg gatttctctg gtgttgtttc ggccgttggg aaaggtgtca gagacctgcg
      841 tgttggcgat gaggtcttcg gcgtctgcga cgttggccag gagggcgctt atgccgagaa
      901 gattgcgatc aaggcagcca tcgtcgcaaa gaaaaccgat cgcctgtcac atgtcgatgc
      961 cgctgctctg gcgctcgctg gtttgacggc gatttgcgca atcgaagaca cattaaaagt
     1021 gaaggccggc gagacgatcc tgatccaagg aggagcaggc ggtgtcgcga gctttgccgt
     1081 acagttggca aaacatctgg gtgctcgcgt gatcacgacc gcaagcgcgt cgaatcatga
     1141 ttatctgcgc aaaattggtg ccgatgagat catcgactac aatgcggtag attttactaa
     1201 ggtcgtgaga gactgcgacg ccgctttcga caccgtcggc ggagatgtcg cgcagcgatc
     1261 gttcactgtg ctgcgagcag gcggccgggc ggcctttatt gcctctggag cacaggcacc
     1321 aaagccggac cgcagcgatg tcgttgctct gcggcccagc gtcggacgtg atcggcaaca
     1381 tctcgagcgc atcgtggcgc tggttgcact aggggccgtc cgtcccttgg aaataacatg
     1441 ctatcagctc agcgaagccg tggccgcgca caaggtaagc gaatcccgcc actttcgcgg
     1501 caagctggtt tttcaagtcc gttgaaaaca gcctcgtttc gccccgccgt ttccgacggg
     1561 aagaaattcg aatcacgcag tcgcttgagc agcggtgagt ggcgacctcc gttcatgtga
     1621 aggttttcgg atgccggccc catgaaggtg gcgccgcgca cgccggggcc ggcgtccgca
     1681 aaccttcaca gaagcggcca caacaaagaa cattcggatt cgaagctcca tcgtattaaa
     1741 gggacctgat ttcacggcgc gaggcttttg acggttcacc agctccgtca ttagccttcc
     1801 acttcctcga acgcctcgcg cgcgtttcgg aaggcatcga ttcctgaggg gatgccacaa
     1861 tagaccgcga cctgaaggag tatctccttg atctcgtcct tggtgagccc gttcttcaag
     1921 gcccctttga cgtgcagctt caattcgtga ggccgattga gagcgccgag cattgcaaga
     1981 ttgacgatgc tgcgcgtgcg gcgatcaagc cctggccggg tccacagcgc gccccagcaa
     2041 aactcggtcg accattccgc catcgtccgc gtgaattcgt ctgccccggc aatggatttg
     2101 ttgacgtagt cctcgccgag tacttccttg cgaaccgtca ggcccttttc aaacaaactg
     2161 ctgtcgctca tcatcaatcc ttccaatgct tgagttctag ttttttctgc gtgccgaacg
     2221 aatcggcgca gatatcgtct ccccatcgcc ggcgttgggc ccgctatgcg agctcatcaa
     2281 gcccgataca ttggcaagcg catccaggtg ccagacattt tcaataatga tgtttacatt
     2341 ccatgcggtt tcgctgagcc gcgaacagtc gcgcagcttt gcctcggcgc gcgcccaacc
     2401 gagcggcccc tcgatcgcgc gtgctgaagc agcatagtgc ggcgacggga gcagccggat
     2461 ggatgatcgt gtccaggtgg gtatcgtcga aatcgagcag gttggccgag atcgcattga
     2521 cgaatgcagc gccgagcgca tccaggcgct ccggtcggcc gacaatggcg gatgttgcag
     2581 cgccgctgaa tggagacaac gtccgcagcg ctgctgtgat ggccggatca taggccgaac
     2641 cgagcgcggt cgcgaagaaa ttcaggatcg accgtttggc ttcatgactt tgcgcggcga
     2701 catccgccca tgatgtggcg gcggcgaaat tcgcaagcgt tgcaccggcg ccctgccctt
     2761 cgtcctgccg cactgagcga attcctcgat tgacgttttt caaatcctat tccggtccgc
     2821 tgcgacctgt cgataccaaa tcttatgctt gacagattaa ctgacaatct tgacattcgg
     2881 ggaaagtggc tcgggagccc gtgctgcgac aagaacaaca agagcgctcg cctcatgcgg
     2941 cgcgacaggg agtgaaacga tggcggtaga gacgatcggc tttgtgggaa cgggccgaat
     3001 gggcgggccg atggccgagc gactactgga cgccggctat tcgttgtgcg tttacgacac
     3061 gcagagcgaa gcaaccaaac cgcttgtcgc gcgtggcgca tgtcttgcaa aatcgcccgc
     3121 cgaggtcgct tctgctgcgg atatcgtgct gaccagtctg ccgactcctg acatcgtcaa
     3181 ggccgttgcg ttgggacctg acggaatcat tgcgggaaac cgcgccagca tcgtgatcga
     3241 tttgtccacc accggaccca gcgcggccaa actgatcgcg gaagggttga agcccaggag
     3301 cctgacgctg gtcgatgctc ccgtgagcgg cggcatcaag ggcgcggtga acggcacgct
     3361 ggcggtcatg gtgtcctgcc cgaaagcaac ctatgaaacg gttcagccga tcctgaagca
     3421 cttcggaaag ctgttttata ttggcgacaa gccgggcacg gcgcagaccg cgaagcttgc
     3481 caacaacctg atggcagccg ccgccctcgt gatcacgtca gaagcggttg cgatgggtgt
     3541 caagggtggc gtcgacgcca agctgttgat cgatatcatc aatgccagca gcgggcgcaa
     3601 cagcgcctcg gaagacaagt ttccccgcgc cgtgcttccc ggcaccttcg atttcggttt
     3661 ttccaccggc ctttcctaca aggacgtgcg actttgcatc gatgaagccg aggcaatggg
     3721 cgtgccgatg gtctgtggtt cgatcgtccg gcagatgctt gcgattacca acgccaaata
     3781 cggcgcgtca tccgacttca cgtcgatcgc gaaagtcctt gaggaatggt ccggcgtgga
     3841 aatgcgcggc tagattcgga cagcagccga gcgaggggcg attgcaatga cgatcgggtc
     3901 gaccgggcca agcgtggtgt ccctggcgcg gcagttggcg cgggaatcgc ttgccgtcga
     3961 tctcggccgt ttcggtgcgg acgtcgtcgc caaggccaag aactgccttc tggattttct
     4021 ctcctgcgcg tttgaggcac gaaatcaccc atggagccgc caggcgatcg gaatcgcgcg
     4081 cggaatcgaa aatggcgcaa ccattatcgg gacccgcaaa ctttccacac cgggcgacgc
     4141 ggccttcgcc aacgcaacgc tgggccacgg tctggtgcgc gaagacatgc acgccgccag
     4201 catctgtcat cacggcgtgg tgctctggcc caccctgctc gcattgtcgg aacgaacgcg
     4261 aacgcccggc accacgttgc tcgcggccgc gatcgttggc tacgaggccg gagcgcagat
     4321 cggccgcgca ctctttaccg ccgatctggc gcgccttttt aggccgaccg ggctcgtcgc
     4381 gccgctcggc gccgcgctcg ctggaagccg cgccctcggt ctgaccgagg atgccgcgac
     4441 aagtgccgtt tccattgccg ccaacacatc gtccggcctg aacgaatggc cgcgcgccgg
     4501 cggctctgaa atgtatttcc atccgggttt tgccgcccgc aacgcgatcg cagcgatcga
     4561 actcgcggaa gccggcgcct gcgcctcgga aacgatcctt gaaggtgagg ccgggctgtt
     4621 cgcggcctac cgccgacagc ccgcgcccgt cggcatccgg ttgttcgccg gccagccaga
     4681 aatcatggcc gtctacaaca agccggctcc tgcctgcaat ttcgcgcaaa ccgccgcgca
     4741 ggcagccttg cgcaccgcgc gcgaactgaa gaggtcggaa gatatcgaga ccgtttcgat
     4801 acgggtcccc gaggccgcgg ctcgttatcc cggctgcgat tccaaaggac ccttccagaa
     4861 tgccctgcag gccaagatga gcattccctt cagcgtcgcc gccgtgctcg cgcgcggtgc
     4921 actcgaagaa gacaattacg cgcatatcgg ccatccgcaa attcttcgcc tcgtcgagcg
     4981 caccgatctg caaagcgagg ccagcctgac tgcggcgttc ccggcaaacc agggcgccga
     5041 agtccttgtt cgtctgcgtg atggcaacac catctgtcaa cgtctcgaca atgtcattgc
     5101 cgccacgccg caggaaatcc gcgcccgctt tcgacgcgcc gccgtcgacg tcgtcggcga
     5161 caaatgcgca cgacatttgg aagagctcgt cgaccgttgc gaatcccttt cggacagccg
     5221 cgtgatcgcc gcactgtgcc ggcttgagcc gtcggaacga cgaccggcgt catgaccaat
     5281 aatggcaaac agaagaaaat ggggggtgca atgttcaagc cgcagacagc ctcgacaccg
     5341 gcaaccggcg tgaggcaagg gaactcgatt cctgcgcgag gccacgggtg agccaagcgc
     5401 tggaaggctt cctgcaggcc ttgaccgccg gacttttgat cggcgcggtc tatggcttga
     5461 tgtgcgttgg gctcgggctg atcttcggcg tcatgcggat catcaacttc gcccagggcg
     5521 atttcatgat gctgggcatg tatgcggcgt tctatctttt caccgcgctc ggcattcaga
     5581 ccacgttcgg caatgccatc ggcccgttcg tcgcaatcct gcttgccggt cctgtattgg
     5641 ccgtcttcgg ctatgcgatc caccttgcct tgatctcgcg cgtatcgggt acgcgcacct
     5701 cctcgctcga gggagaaggc cactatgccc agctcattct gacgctgggg atcgcgctgg
     5761 tcctgcagaa cggcggtctg atcgtgttcg gttcggtgct ggcctcgatc cgcacgccgc
     5821 tgtcgagttc ggcctgggaa ctcggcccgt tcttcgacat cagcgtgttc gtcaacaagg
     5881 cgcgcgggat cgacgccgtt gtttcgttcg caacgatgat actgctgacg ctattgatca
     5941 cgcgatcgcg gatcggaaaa tcgatgaggg ccgcagccga caatcccact gcggcaacct
     6001 atatggggat cgacgttgat cgcgcgcacc gcgtcgcctt cgcgctgggc accggcatca
     6061 ccgcgatcgc cggtggcctg ctcgccacca actatccctt tcatcccttc gtcggcgtcg
     6121 aatacgtcat cgtcatgtat gcgggggtcg tgctcggtgg catgggcagc attatcggcg
     6181 ccttctgggg cggcatgacg attggcctgg tacagcagat gtcgaccctc gtgctgccga
     6241 cgcaactgca gaacgcggcg atcttcgccg tctttctcct gatcatcttc ttccgcccac
     6301 agggattctt cgggcgcatc gtcgagagga cgtgacgatg cggggattgc ggccgctgct
     6361 cccggttctt gtcttcacgg cgctttatgc ggcgctgtcg ctgagcgtca ccaattcgta
     6421 ctaccagctg gtgatgacgc tggttccggt ctgggcggta ttcggcctgt cgtggaacct
     6481 gctcagcgga tacaccggac ttatctcgtt cggccatgct gccttcttcg ggatcggcgc
     6541 ctatgtgacc gcgctcggcc agatctattt tgatttgtcg ccatgggtat tgatcccggt
     6601 tgcggcaacg cttggcggcg tagccggact gctaattgga tttccgacat ttcggctgca
     6661 ggggcactac ttcgcgctgg cgatgctcgc ctacccgctt gccattctct acgtgttcga
     6721 atggctcggc tttcaggaag tcaccctgcc gatcaagcgc gacaatccga ttgcctacat
     6781 gcaatttgcc gatccgcgcc tctatacgtt gctggcagtg gcgatgatgc tagggacgat
     6841 cctgctcacg cggatcatcg aaaattcacg tttcggcatg gcgctgcttg ccatcaagca
     6901 gaatgaggcg gctgcggaag ccgctggaat cgacacgctg gcctggaagc tgcgcgccat
     6961 cggcttgagc ggggcgattg ccggggcgat cggcggattc tacgccgtcg tgctgctggt
     7021 ggtgaccccg caatcggtat tcggcatgct ggtgtccgca caggcgctga cggttgccat
     7081 gtttggtggc gtcggaacgg tctgggggcc ggtgatcgga tccgtgatcc tgatcccgct
     7141 ggccgaaacg ctcaacgccg aagcaggctc gcgccttccc ggcattcagg gggtgatttt
     7201 cggcctcgcg atcatatccg tcatcctcgt cgccccggag gggttgttct ggaagtttcg
     7261 cgatttcctg cgcaggcgat cggcgccgca agccgcggcg ccgtcgagca cgccagatcc
     7321 ccggctcgcg gacgccgtcc catccgcgcc gcagcgatcg cgttccgaac ggacccaggc
     7381 gaccggcgat gtcgtccttg aggtgcgtaa cctgtcgcgt tccttcggcg gattgaaagc
     7441 cgtccaaaac gtcagcttca aactgcggca aaacgagatc cttggaatta tcggccccaa
     7501 tggtgccggc aagaccacgg tgttcaacct gatgaacgga ttcctgcggc caggcacggg
     7561 tgaaattctc atcaatgggc gcgacatgtc aggccgcaag ccgcacgagc tttgtgaagc
     7621 aggcgtcggg cgaaccttcc aagtcatgcg cccgttcttg cgcatgtcga tctcggacaa
     7681 tgtcgtggtc ggcgcctatg tccgggctgg gaccgacgcc gaagcaagga aactagcggc
     7741 ggatgcgatt gcccgtgtcg gcctgtctca catcgccgac cgcatcgccg gcgaactcac
     7801 gaccaaggaa ttgcggctga tggagctcgc ccgcgcgctg gccgggcagc cgcgcatcct
     7861 gctgctcgac gaaacactgg caggcctcgg acatgatgaa gcagacgagg tcgtcgcggt
     7921 gattcaacgg ctcgcccgcg acggcatgac catcgccatc atcgaacaca cgatgcaagc
     7981 catggtccgt ctggtcgaca gtttcctggt tctcgaccac ggcgccgtta tcgtcgaggg
     8041 agagccggaa gccgtcaccc gcgacagccg cgtcatcgaa gcctatctcg gcaagaaatg
     8101 gatggcgcat gctccgcatt gaagggctga ccgccggcta ttcggcaatc cccgtcttga
     8161 acggcgtctc gatcaaggtc gaacggggcc agttcgtcgc catcgttggg ccgaacggcg
     8221 ccggcaagac caccctcttc aaaacgattt ccgggatcgt gcggccgagc tccggagcga
     8281 tcacattcga agataccgac ctcttgtccg tccggcctgc gcgacgcgcg catctcggca
     8341 tcgcccacgt cccggaaggc cgccaggttt ttccatcgct cacggtaatg gaaaatctcg
     8401 aaatgggcgc gatgaccgat gctggccgac gcgactggaa acgcaatatc gagcgcatct
     8461 tcgaatggct tcccgtgctc gccgagcgcc gcggccaatt cgcgggaact ttgtccggcg
     8521 gacagcagca gatgctcgcg atcggccgcg ggctggcgtc gtcgcccaag cttctgatgc
     8581 tggatgaacc ctcgatggga ctggcgcccg cgattgcaga cttcatcttt gaacggctga
     8641 tcgaaatccg ccggcaatcc aacctgacga tcctgctggt cgaacagcgc gtggcggaag
     8701 cgctggagtc cgccgatcac ggctacgtcc tcgaggctgg ccgcgtcgcg ctcgaaggca
     8761 acaatcagac attgcgcgcg gacgaccggg tccggagggc ctatctcggc atgtgaccaa
     8821 cgatcaatca acgaacgaac cgggaggata aaaatgagcc aacaaaaaat aaccgacaga
     8881 acgtcgagga cgtcggtgac acggcgaacc gtgctctccg gcgcagccgc catgggacta
     8941 tcgacggcgg cgcgagcgca gcaaccggcc gaggtcaagg tcggtctgat cgtgccgctt
     9001 tccggcatct atacccgtcc gggccaggtg atgcgcatgg gcgccgagat gggcatcgaa
     9061 cacatcaatg cgcaaggcgg catcaagtcc ctcggcggcg ccaagctcaa actcgtcgtg
     9121 atcgattgcg gagacaccac ggaaaaggcc aagaatgccg cgcagcgcat ggtggcgcag
     9181 gaaaccgatc tcgttgcggc gaccggcgcg tatctcagct ccttcacgct ggccgtgacc
     9241 gaggtgaccg agcgcgccga actgccgatg ctgacgctgt cctattcgga tctactgacc
     9301 gagcgcggct tcaaattcat cttccagacc gcggcgccc
//
LOCUS       DASWGM010000426         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1709529,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000426 DASWGM010000000
VERSION     DASWGM010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709529"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VGA15_12420"
     CDS             complement(<1..232)
                     /locus_tag="VGA15_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9588530.1"
                     /translation="MPDRIHELLHRNLQEVFGESNVVRRRAAIEDLYTEDCVLYAPPG
                     VFVGPEALDRFAGDLRATHPHFVYTPRGEAQAL"
     gene            complement(269..901)
                     /locus_tag="VGA15_12425"
     CDS             complement(269..901)
                     /locus_tag="VGA15_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX9588531.1"
                     /translation="MKLLHIDSSITGAGSASRQLSAALVAALKAAIPGLGVVRRDLDA
                     NPIPHLDSKRLPTVRPANAPEGAVGIADDGGSDALDEFLAADILVIGAPMYNFGIPSQ
                     LKAWIDRIAIAGKTFSYTADGPIGLAGGKHVIVASSRGGLYAPGMPFEANDFQEKYLR
                     AVFAFIGIGDIEIIRAEGLALSPAHREAALNAARATIPTTVAAARPRHAA"
     gene            complement(948..>1581)
                     /locus_tag="VGA15_12430"
     CDS             complement(948..>1581)
                     /locus_tag="VGA15_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9588532.1"
                     /translation="DGPNFCMGGNVPEWPGKSADWFRTFIAECNSSFHAIEALRIPTV
                     AVVRGGAIGGGFELALACDFLVAAEDATFFAVEIRSGNIPLAGGLQRLAERVGRARAA
                     KLALLGEPFSGIIAGDLGIATLVVPEADLDETSRNLVNKLANGPTKAYAAARTLLKAW
                     SAGGVAAADVMMLDVAIDLFKTEDTTKGILNSAKAIQAGVQPSPLTFLGR"
BASE COUNT          294 a          494 c          493 g          300 t
ORIGIN      
        1 gaagcgcctg ggcctcgccg cgcggggtgt aaacgaagtg cgggtgggtc gcccggagat
       61 cgccagcaaa tctgtcgagc gcttcggggc cgacgaatac gcccggcggc gcgtacagca
      121 cgcagtcttc ggtgtagagg tcctcgatcg ctgctcggcg gcggaccaca ttactctcac
      181 cgaagacctc ttgaaggtta cggtggagca gttcgtgaat acggtcgggc ataagatatc
      241 tccgtagagg gatgcgccgc cgtatccgtc atgccgcgtg acggggtctt gccgctgcaa
      301 cggtggttgg aatggtcgca cgggcagcgt tcagcgccgc ttcccgatgc gcggggctga
      361 gcgccagccc ttcggcgcgg atgatttcga tgtcgccgat gccgatgaag gcgaagaccg
      421 cgcgcagata cttctcctgg aagtcgttcg cctcgaacgg cattcccggt gcgtaaagac
      481 caccgcgcga cgaggcaacg atcacgtgct ttcctccggc caacccgatc ggcccgtccg
      541 ccgtatagct gaaggtcttg ccggcaatcg cgatgcggtc gatccatgcc ttgagttggc
      601 ttgggatgcc aaagttatac atcggtgcgc cgataacgag gatgtcggcg gcaaggaact
      661 cgtcgagcgc gtcgctacca ccgtcgtccg cgatccccac tgcaccctcc ggtgcattcg
      721 ctggtcggac ggtcgggagg cgtttgctgt caaggtgcgg gatcggattc gcgtcaaggt
      781 cgcgccggac cactccgaga cctgggattg ccgctttcag tgccgcgacc aacgctgctg
      841 agagttgccg cgaagccgag ccagcgcccg taatgctgga gtcgatgtga aggagtttca
      901 tagtcttcgt cctggtcaac gtttgtgcgg atgcccaagt ccgggcgcta tcttcccaaa
      961 aatgtcagcg gagacggctg gacgccggct tggatagctt tcgccgaatt gagaatgcct
     1021 tttgtcgtgt cttcggtctt aaagaggtcg atcgccacgt cgagcatcat cacgtccgct
     1081 gcggcgacgc ctcccgccga ccatgctttg agaagcgtgc gcgctgccgc ataagccttg
     1141 gttgggccgt tcgcgagctt gttcacgaga ttacggctcg tttcatccaa atcggcttca
     1201 ggcaccacga gcgtagcaat acccagatcg cccgcgatga tgccgctgaa cggctcaccc
     1261 aacagagcga gtttagcggc gcgggcgcgc ccaacccgtt ctgcgagccg ctgtagcccg
     1321 cccgccaacg gaatgttgcc cgaacgaatc tcgacggcga aaaatgtcgc atcctccgcc
     1381 gcgaccagaa agtcgcaagc aagagcaagc tcgaacccgc cgccgattgc tccgccccga
     1441 accaccgcca cggtgggaat gcgcaatgcc tcaatggcat ggaacgaact gttgcactcg
     1501 gcgatgaacg tgcggaacca gtcggcgctc tttcctggcc actccgggac attgcccccc
     1561 atgcagaaat tcgggccgtc c
//
LOCUS       DASWGM010000427        11870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_166609,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000427 DASWGM010000000
VERSION     DASWGM010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166609"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(880..4476)
                     /locus_tag="VGA15_12435"
     CDS             complement(880..4476)
                     /locus_tag="VGA15_12435"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9588533.1"
                     /translation="MARPLKIFISSPGDVADERRRAALVISLLKREFARFLDISAVLW
                     EYEPMLSSGHFQDIIDPPSSADIVVLILWSRLGTPLPARTETREYKSLAGEGPVTGTE
                     WEFEQAVEARKRQNGVPDLLVYRKFADGVAKFSRAEELEGIRQQWEALQAFWRRHFED
                     PDGRFKAAFNRFATLDEFQSQLDQHLRELLRRRLPPQPLRIAGAKECDRIEWWSGSPY
                     RGLQAFDMEHAVVFFGRERAEREISEALVRHAGQGKGFMLVVGASGSGKSSLVRAGLL
                     PDLIAPGVVGGVSTWRHLIIQPTELAPDPFVGLARALLRPTALPELAAVGYLETELVA
                     QFRSDPALASVPLRVALQRAAAEDQQAPPGVVRQGRLVLVLDQLEGLFTSAELSDDVR
                     RALDLLLAKLAQSGVIWVIATLRSDFYHRLVDLPELNALAAGLGQYLLAPPNAAEIEQ
                     IITRPAEVAGLSFEIAEDSGISLAAAIRESAARDPASLPLLSFVLDELYRRDVGERGN
                     ILTYASYQALGRLEGAIAGHADALVEKLTPEMKSALSDLLLALVEVDEFKATATARTV
                     ARSALADPKQRELADQLVLARLAVADDSGGGVTLRLAHEALLSHWPRIERLIDEHRDF
                     LIVRRRLQGDAATWRNHARHADYLLPAGRRLAEAEDALAHRCGQLDPEIIAYAEASVA
                     AEQERVAAAQRAKEAALRRELKRSRQFAAVVSVLLVLAVAAGIYAWQQRRIASDALAQ
                     AQANYQIALDQAAGSVALLTDSYGDGEISTKLMEQLVDRAQKTINSLPGETDDVTAAR
                     AQLLDVLSLAHFALGNISDAQKFGEDELKLANALVAKAPANPRWKRLEAAAHGRLSDV
                     LYWKGDPVGALREGSLGRDISAALAAAQPDDDELQRDLSNQYRRMGDGFDSKGDLEDA
                     RAAYRPWVEIAMAQLAKTHDDPKWLSSLAFAHREIGDTLAQESRPAEAAIEYRSSVQI
                     SSQLVMRFPRNTAYLYTLASSRLRVGDALLAQGESASALVEYQVTKELALKLLKSEPS
                     NFRWRQLLQAGYQRVGDLYLHDKNYGSALEEFHTYVALAVETQSSIPNNGSALFDVTN
                     AHQKVGDALREQGKLDDALKAYQGSLRVAVEINNMNFWNGAWKKMLAMDYQRIGMVYK
                     LQGDNGNAFAHFQKCLDVPVNNFAWSPRTLWPADVTDFCRQAITELGGKPTR"
     gene            complement(4571..6505)
                     /locus_tag="VGA15_12440"
     CDS             complement(4571..6505)
                     /locus_tag="VGA15_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588534.1"
                     /translation="MSIRRIVYWAWQTVSVARGRFAANLVSSVLAQQLSLYRSQLLVT
                     VIALLSAGGAGSAVTSGQESPGGFLARFMPQDLTAAAILFAVLAVVVVLMQFGDRVLA
                     TSTDMVMLGRFQQRLHDKLLVLGNNFHQRVGLSDATMIVTRYAMTVQSLMREVASFPF
                     VRGLTLLTAMIYLLNNLSMLGNPPVWVPFLLLGIVVIFPIVGWRLSIMLRSAFQRSTL
                     AEMAVAKEFANSDALPLEIQVMGANEQRSKAFTLRVADAVKAKVRAAMRNEVAYQFEN
                     ATPDFIQAGLLIYGVFQTLSLDNIHDRAIAGAAIIGLIQFVPQVVSPVRQMIDMANMV
                     NTLWPQVEMAIQVLEAEPEIKEPSNPQQLPQGPLKVEVKDLSYKPLPDAPPILDNVSV
                     VFGSGEIWGLVGRSGSGKSTLLNLVARLREYQRGTLRLGGHDIRNVALGNLRRSIGMV
                     SQFPLIINDTARANLRLARADATDQKLEAVCRSVGVWDALQNSAPPGGSGLDVQLYTE
                     SNKGALSGGQRRLFAIARALLNEPGILILDEPTTGVDTLTRLPLEEVVRSHSKGRTVI
                     MVDQDMGFVAALADKIACLEGGRVADVVEKEEFLTKPSLFLKLYKASQRSSESHMQAV
                     AVLDAVDQKSVPTDGAMKKG"
     gene            complement(6782..7024)
                     /locus_tag="VGA15_12445"
     CDS             complement(6782..7024)
                     /locus_tag="VGA15_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588535.1"
                     /translation="MASEIKAKIGTKISEAEQKGLDPKKVGKLQGKHPKGGDVEAQAA
                     LAFQVCPYCGSAGWGAESAFVYLYFTCHACGRTFKA"
     gene            complement(7177..7950)
                     /locus_tag="VGA15_12450"
     CDS             complement(7177..7950)
                     /locus_tag="VGA15_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX9588536.1"
                     /translation="MLAAPYWPKGWDSIREQALAPVTVAVLDSGVDGSHPDLAGRVVG
                     AVAVEIDNGKPVVHELSPEANNDIFGHGTPVAGIIAAIAPNARIYDVRIFSEKSIGAK
                     RILLTGFDHALSQPWRLLNMSLAAVSSIRRELVDLCERAYFQQQIVVAARRNAPFEDD
                     GLPAELSSCIGVDRGAYPSPFQYVYRPRTPIEFEARGETVVAPAKGGGYTTLSGTSFA
                     TPTVSALCALLLGAYPDMTLFELKTSLRQLAQTAKNGSD"
     gene            complement(7950..8612)
                     /locus_tag="VGA15_12455"
     CDS             complement(7950..8612)
                     /locus_tag="VGA15_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9588537.1"
                     /translation="MQLGSEGLLTEYLGLLPATPEAAALRQIVEIGVGVIQADEGSLL
                     VMDADGGDLRFAMTVGNGDSEKTLLGQRVPIGSGITGLAAATRQVQVGAPVYKDVAQT
                     ERLSEGPESVIAAPMLLGELVVGVLTAVTFKKGRIFNSQEAVLYARFARLAALLVEQT
                     RRLKVRGGKGPQARALGGMARLEQEVLGRMQRIVAERPGALGPLARILEAVEEISAGG
                     SL"
     gene            complement(8733..9443)
                     /locus_tag="VGA15_12460"
     CDS             complement(8733..9443)
                     /locus_tag="VGA15_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9588538.1"
                     /translation="MIMIPRPASDKRELLSRHFLLKDLDVRTLDRIVHYSLVRSVSEG
                     SVIFSQGDLGECLYGILRGRVRIFNNSPENREILLNILEPGALFGEIALLDDRPRTAN
                     ASAMEDCELLTMHRQHFIPLLEEDPRLAIHMLGLLCARVRWTSSIIEDTAFLNLPARF
                     AKRLLALAHSHGEKLPDGERIRINLRLSQRQIGAMIDASREAVNKQIQIWRADGILDL
                     DDGFLVLRRPDWLEAIVG"
     gene            complement(9673..11466)
                     /locus_tag="VGA15_12465"
     CDS             complement(9673..11466)
                     /locus_tag="VGA15_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEX9588539.1"
                     /translation="MTDTLPSDWIDRVRTAERSGDFLVAYDIATQGLAEHPDSVDLRY
                     LATRVLARSGATDQAAALYKRFELERERKLDFATLGARIAKDGALAAPADRRTALRAA
                     AAAYGRIYARTPDHYPAVNAATLYLLAGDPQRARLYARRALRASDRGSDRTSIERYYR
                     LASRAEAALICGDAERARQALLQAARYLSGNFDAAASTRKQLRLVCRATRTSPDILDL
                     LRPPAVQHYYGPALAGHASRHLPDRTRERDIVKKIVDHFTQHGIGYAYGSLSAGSEIL
                     CTEACLRAGAELHVVLPFNKDEFVATMVRVAGSSWVRRFKACLDRAKSVTFATGDAYQ
                     GDNSLFTYACRLAMGMAILRAQHLDTEVSHLRLQIGGWDPDPAGYASDLRMWHAQDLA
                     SRVLVLTESSVAIPHVQKRPTPANRMPPRFSRALLFGDVKGFSKTPDHLIPVFQKRMM
                     GTIAGVLRRYGRHVLYRNSWGDAIYVVIDDPIVAAECCLAIQEAITRAKTAGYGLSPE
                     LALRLSAHFGPVYDGHDPIRDEPTFFGAHTTLAARIEPVTPPGNVYVTEAMAAAIAMA
                     NAPRLRAEYVGNVPMAKGFGSTRMYSLNRVG"
     gene            complement(11689..>11870)
                     /locus_tag="VGA15_12470"
     CDS             complement(11689..>11870)
                     /locus_tag="VGA15_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588540.1"
                     /translation="RVRARIKMPDASGLAYGGALYARPRTLVSFNSTVAVWRLLAWRG
                     SAESHRSLRGHRFSD"
BASE COUNT         2263 a         3871 c         3620 g         2116 t
ORIGIN      
        1 atctcgaaca caaatagcgt ccggttgccg gtttacaccc tccggttccc agcggtttcc
       61 aggctgcgca gggggaaaag caaaaccccg ccagcatgtg attggcgagc aaaacagggt
      121 gcactcccca atctgggaga catcttcgcc cacggaggcg ggtccaagtc gccgggagtc
      181 gcttgcgcct gctcgtacgg gctttcacgc gcgtgggcac gtctgccaac caacgagaag
      241 gccccggcag cgagtgccgg cccggctcat gggagcccgc gccagggcct aagccgggca
      301 tcgctgcgtg ccggtcgaac ccgaagctgg gcccgcgtcc aggagcgcgt tcctgccctt
      361 ggtccgagcc cggtacggcg cgcagacccg gggctggaat cccaagtcat ttcaacctcg
      421 cgcgtgaagc gatcgtggcg gctgaccagg ccgcgatcgt tctcgagcat agcttcttct
      481 ggaacccggc caaaatgccg aaaggcgcgc cgtcgaatcc agcgaaccag ctcccctggc
      541 gctcatcacg gaacgtccgc aaatgcagcc ggcggcgata gcccaacgtc gccacgaaca
      601 agaatatctt cacgctcgcg tcccccggca tcgagcggac gggcgaggct gttgctctcc
      661 aggaaggaac ggagcgccag cacttgagct tggggcaaga cggagctttg ccaaggtttc
      721 cttcgatctt gcgccaggtc gaccggccgc ggcgtctcat gcaggccgca gagatcgcgt
      781 cggagatccc ctggatcatg atcctgtaaa ttgccccgcg gccctcagcc cgcgcgcgtt
      841 tgacgttttg catggacaac ccccgactgg gcggtgacgt cagcgcgtgg gcttgccgcc
      901 cagctcggtg atcgcttggc gacagaaatc ggtcacgtcc gccggccaca atgttcgtgg
      961 gctccaggcg aaattgttga ccggcacgtc cagacacttc tggaagtggg caaacgcgtt
     1021 gccattgtct ccctgcagct tgtacaccat gccgatccgc tgatagtcca tggcgagcat
     1081 tttcttccac gccccattcc agaaattcat gttgttgatc tcgaccgcga cccgcaggga
     1141 gccttgatag gctttgagcg catcgtctag cttgccttgc tcgcgcaacg catccccgac
     1201 cttctgatgc gcgttcgtga catcgaacag cgcactgcca ttgttcggaa tgctgctctg
     1261 cgtttctacc gccaaggcaa cgtatgtgtg gaattcctct agcgccgagc cgtaattctt
     1321 gtcatgcaaa tacaaatctc cgacccgctg atagccggcc tgcagcagct gccgccagcg
     1381 gaaattgctt ggctcggact tcaagagctt gagggccaat tccttcgtga cctgatactc
     1441 gacgagcgca ctcgcggatt cgccttgcgc cagcaaggcg tcaccgacgc gcaagcggct
     1501 agaggcgagc gtgtacaaat acgccgtgtt gcgcggaaac cgcatcacca attgcgatga
     1561 aatctgcacg ctagaccgat actcgatcgc agcttccgcc ggccgggatt cctgcgccag
     1621 cgtatcgccg atctcgcggt gggcaaaagc aagactggag agccacttgg ggtcgtcgtg
     1681 cgtcttggcg agctgagcca tcgcgatctc gacccaaggc cgataggccg ccctcgcgtc
     1741 ctcaaggtcg cctttcgagt cgaacccatc ccccatccgc cgatattgat tgcttaggtc
     1801 ccgttgcaac tcgtcatcgt cgggttgcgc cgccgccagc gctgccgaaa tatctcgccc
     1861 aaggctgccc tccctaagcg cgccgaccgg atcgcccttc cagtagagaa cgtcgctgag
     1921 ccgaccgtgc gccgcagctt ccagcctttt ccagcgcgga ttggccggag ccttggccac
     1981 cagcgcgttg gccagcttga gctcgtcttc gccgaatttc tgcgcatccg agatattgcc
     2041 gagcgcaaag tgggcgaggc tgaggacgtc gagcagctgc gcacgggcag cagtgacgtc
     2101 gtcggtttcg ccgggcaggc tattgatggt cttctgcgcc ctgtcgacca gctgctccat
     2161 cagcttcgtc gaaatttcgc cgtcaccata actgtcggta agcaatgcga cgctacctgc
     2221 cgcctggtcg agggcgattt gatagtttgc ctgggcttgc gccaatgcat ccgaggctat
     2281 tcggcgctgc tgccaggcgt agatgcccgc cgccaccgcc agcaccagca gcacgctgac
     2341 gaccgcggcg aactggcggc tgcgcttaag ctcgcgccgc agcgcagctt ccttggcgcg
     2401 ctgtgccgcc gcgacgcgct cttgctcggc ggcgacggaa gcttccgcgt aggcgatgat
     2461 ctccgggtcg agctggccgc accgatgcgc aagcgcatct tccgcctcgg ccaggcgccg
     2521 ccccgcaggc aacaggtagt ccgcgtgccg agcgtggttc cgccaggtcg cggcgtcccc
     2581 ctgcaatcgc cggcgcacga tcaagaagtc gcgatgctcg tcgatcagcc tttcgatgcg
     2641 tggccagtga ctgagtaacg cctcgtgggc aaggcgcaac gtcacccccc cgccgctatc
     2701 gtcggcaacc gcaagccgcg ccagcaccag ttggtcggcg agctcacgct gcttcgggtc
     2761 agcaagggca gaacgggcga cggtgcgcgc ggtcgccgtg gccttgaatt cgtccacctc
     2821 gacgagggcc agcagcaggt cggaaagcgc ggacttcatc tcgggcgtaa gtttctcgac
     2881 tagcgcgtcc gcatggcccg cgatggcgcc ctcaagcctg ccaagagcct ggtagctcgc
     2941 gtaagtgaga atattgccgc gctccccgac gtcgcggcga taaagctcgt ccagcacgaa
     3001 ggacaacagc ggcagtgatg ccggatcgcg cgcggctgat tcgcggatgg cagcggcaag
     3061 gctgatcccg ctatcctccg cgatctcgaa ggacaggcct gcgacctcgg ccggacgggt
     3121 gatgatctgt tcgatctcgg cggcgttcgg cggcgccagc agatactggc cgagcccggc
     3181 cgcaagagcg ttaagttccg gcaaatcgac gagccgatgg tagaaatcgc tgcgcagcgt
     3241 ggcaatcacc cagatgacgc cgctctgtgc gagcttggcc aagaggagat cgagcgcacg
     3301 gcgcacgtca tcggagagtt ccgcggaagt gaacaggcct tccagctggt cgagtaccag
     3361 caccagccgg ccctgtcgga ccacccccgg aggggcctgt tggtcttccg ccgcggcgcg
     3421 ctgcagcgcc acacgcagcg gcaccgaggc cagtgcggga tcggagcgga actgggcaac
     3481 aagctccgtc tccaggtagc cgacggcggc aagctccggc aaggcggtcg ggcgcagcaa
     3541 cgccctggca agcccgacaa acgggtcggg cgccagttcg gtcggctgaa tgatcaggtg
     3601 tcgccaggtc gagacgccgc cgacgacgcc gggtgctatg agatcgggta acagaccggc
     3661 gcgaaccagc gacgatttcc ccgaaccgct cgcccccaca acgagcatga aacccttgcc
     3721 ctggccggca tggcgcacca aagcctcgga gatctcacgc tccgctcttt cacggccgaa
     3781 gaagacgacc gcatgctcca tatcgaaggc ctggagaccc cgatacggag atccggacca
     3841 ccactcgatc cgatcgcact ctttcgcacc ggcgatgcgc aacggctgcg ggggcagacg
     3901 ccggcgcagc agttcgcgca gatgctgatc gagctggctc tggaattcgt cgagcgtggc
     3961 gaagcgattg aatgccgcct tgaagcgacc gtccgggtcc tcgaaatgcc gccgccagaa
     4021 agcctgcaac gcctcccatt gctggcgaat gccctcgagc tcctcggcgc ggctgaattt
     4081 tgcgacgcca tcggcgaatt tgcggtagac gagaaggtcg ggtaccccgt tctggcgctt
     4141 gcgcgcctct acggcctgct cgaactccca ttcggtgccg gtgaccgggc cttcgccggc
     4201 gagagatttg tattcacgcg tctcggtgcg cgccggcagc ggcgtcccaa ggcgcgacca
     4261 caggatcagc acaacgatgt cggcggatga aggcggatcg atgatgtcct ggaagtgccc
     4321 cgagctgagc ataggctcgt actcccagag caccgcggag atatccagga agcgggcaaa
     4381 ctcgcgtttg agcaggctga tgaccagggc tgcccggcgg cgctcgtcgg cgacgtctcc
     4441 gggcgaagag ataaaaattt ttagcgggcg cgccatcagt caaaatctcc ctttggctat
     4501 tgcggtggat ttgaagttcc cccatccgtg ccgcgagttc gtagaggaac ttcaaatcca
     4561 aaatccaaca ctagcctttc ttcatcgcgc catccgtagg cacgcttttt tgatcgaccg
     4621 cgtcgagaac cgccacggcc tgcatatgcg attcgctgct gcgctggctt gccttatata
     4681 gcttcaggaa caggctcggc ttggtcaaaa attcctcctt ttccacgacg tccgccaccc
     4741 tgccgccttc caggcaagca atcttgtccg cgagggcggc gacgaaaccc atatcctgat
     4801 cgaccatgat gacggtccgc cccttggaat gcgatcgcac cacctcttcc agcggaagcc
     4861 tcgtcaaggt gtcgacgccg gtggtcggtt cgtcgagtat cagtataccc ggctcattca
     4921 gcagggcccg cgcaatggcg aaaagccgac gctgtccgcc ggacagcgcg cccttgttcg
     4981 actccgtata gagctgcacg tcgagcccgc ttcctcctgg cggcgcggag ttctgaagcg
     5041 cgtcccacac gccgacgctg cggcagaccg cctcgagttt ctgatccgtc gcgtcggccc
     5101 gggcgagccg caggttcgcg cgggccgtat cgttgatgat caagggaaac tgcgacacca
     5161 ttccgatcga gcggcgcaaa ttgccgagcg ctacattgcg gatatcgtgg ccgcccagcc
     5221 gcagggtgcc gcgttgatac tcgcgcaggc gcgccaccag attcagcagc gtcgatttgc
     5281 ctgaaccgga tcgccctacg agtccccaaa tctcgccgga accgaacacg accgaaacgt
     5341 tgtcgagaat aggcggtgca tcaggcaacg gcttgtagct gagatccttg acctcgacct
     5401 tcaacggtcc ttggggaagt tgctgcggat tcgacggctc cttgatttca ggctccgcct
     5461 cgagcacctg gatcgccatt tcgacttgag gccacagcgt gttgaccatg ttcgccatgt
     5521 cgatcatctg cctaaccggt gacaccacct gcgggacgaa ctggatgaga ccgatgatcg
     5581 cggcgccggc gatcgcccga tcatggatat tgtccaggct caacgtctgg aacacgccat
     5641 agatcagcaa ccccgcttga atgaagtcgg gtgtggcgtt ctcgaattga taggcgactt
     5701 cattgcgcat ggcggcgcgc accttggcct tcaccgcatc ggcgacccgc agagtgaagg
     5761 ctttcgagcg ttgttcgttc gctcccatga cctggatctc gagggggagc gcgtccgagt
     5821 tggcgaattc cttggctacc gccatttcgg ccagagtcga tcgctggaag gccgacctga
     5881 gcatgatcga caggcgccag cccacgattg ggaatatcac cacgatgccg aggagcagga
     5941 acggcaccca gaccggcgga ttcccgagca tgctcagatt gttgagaaga taaatcatcg
     6001 ccgtgagcag agtgaggccg cgaacgaacg gaaaactcgc cacttcccgc atcagcgatt
     6061 gcaccgtcat cgcgtagcgc gtcacgatca ttgtcgcatc gctgagcccg acgcgttggt
     6121 ggaaattgtt gccgaggaca agcaacttat cgtgcaggcg ttgctggaaa cgtccgagca
     6181 tgaccatgtc ggtggacgtg gccagcacgc gatcgccgaa ttgcatcagc acgacgacaa
     6241 ccgccaggac cgcgaacaga atcgcggccg cggtaagatc ctgcggcatg aatctcgcca
     6301 aaaatccgcc gggggactct tggcctgaag tgacagccga gccggcaccg ccggccgaga
     6361 gcagcgcaat gacggtgacg agcagctgcg atctatacag cgataattgc tgggcgagga
     6421 cggacgagac caggttcgcc gcaaaccggc cacgggcgac cgaaacggtc tgccacgccc
     6481 aatagacgat cctcctgatg gacatgggga gcgaagaatc gggatccgtc ttttgcgggg
     6541 tacccaggcc ggattttgtc tcaggcgcca tacgtccccc ttacccaaag agcacaattc
     6601 tagcgaagtc gattgtttcc gactattgtc cgctcgccac ggcgcctccg gccgaccgcg
     6661 gtccgctatt tcaaggcatc ctagtgcatt ttccggaaaa atgccgccgc gcgacggcaa
     6721 aaccggcgcc gccctccgaa aggacggcgc cggccggacg gcgttcagcg acagagatag
     6781 atcaggcctt gaaggtcctg ccgcaggcgt ggcacgtgaa atacagatag acgaacgcgc
     6841 tctccgcgcc ccatccggcg gagccgcaat agggacatac ctgaaatgcc agggccgcct
     6901 gcgcctcgac gtcgccgcct ttggggtgct tgccctgcaa cttgccgacc ttcttcgggt
     6961 caaggccctt ctgctcggcc tcgcttatct tcgtaccaat tttggctttg atttcactcg
     7021 ccatttttca gttctcctct ctataacgcg ccgcctcggc gcctactcgg cccggcgccg
     7081 aatgcgtcgg actcctcgca gaacagcgtg aacgtcacgc tgactcttgc cccaaaaatg
     7141 cagcctggct tcagccgcac ctgtgaacta cttcactcaa tcgctcccat ttttcgcggt
     7201 ctgggctagc tgccgtagtg aggtcttgag ctcgaatagc gtcatgtccg ggtacgcccc
     7261 cagcagcaga gcgcaaagcg cggacacggt cggcgtcgcg aagctggttc ccgacaacgt
     7321 cgtgtacccc ccgcccttcg cgggagcgac gactgtttcc ccgcgcgcct cgaactcgat
     7381 cggcgtgcga gggcgataca cgtactggaa cggtgacgga taggcgcctc gatcgacgcc
     7441 gatgcacgac gagagttccg ccggcagccc gtcgtcctcg aacggcgcat ttcggcgcgc
     7501 ggcgaccacg atctgttgct ggaaataggc gcgttcgcac aggtccacca actcgcgccg
     7561 gatcgaagat accgccgcca ggctcatatt gagcagccgc caaggttggc tcagcgcgtg
     7621 gtcgaacccg gtcagcaaaa tccgtttcgc cccgatgctt ttttcgctga aaatccgcac
     7681 gtcgtagatc cgcgcattcg gcgcgatcgc cgcgatgatc cccgccaccg gcgttccgtg
     7741 gccgaaaatg tcgttgttgg cctccggcga caactcatgc accacgggct tgccgttgtc
     7801 gatctcgacc gctaccgcac ccaccacccg tcccgcaagg tccggatgac taccgtcgac
     7861 gccgctatcc aagaccgcca cggtcacagg cgccagagcc tgctcccgaa tgctgtccca
     7921 gcccttcggc caatacggag ccgccagcac tagagtgaac cgccggcgga aatttcttcg
     7981 accgcctcca ggattcgggc aagcggccct aatgcgccgg gccgctcggc gacgatccgc
     8041 tgcatccggc ccagcacctc ctgttcgaga cgcgccatgc cgcctaacgc gcgagcctgc
     8101 ggacctttcc ccccgcgaac tttcagccgc cgcgtctgct ccaccaacag cgccgccagc
     8161 cgcgcgaacc gggcataaag aaccgcctcc tgggagttga agatacggcc ctttttgaag
     8221 gtcacggcgg tcaacacgcc gaccaccagt tcgcccaaca gcatgggcgc cgcgatcacg
     8281 gactcgggcc cttccgacaa tcgctcggtt tgcgccacgt ccttatagac cggcgctccg
     8341 acctgcacct ggcgcgtcgc ggccgcgaga cccgtaattc ccgatccgat cgggacacgc
     8401 tggcctaaca gggttttttc ggagtccccg ttcccgacgg tcatcgcgaa ccgcaagtcc
     8461 ccgccatccg cgtccatgac gagaagcgag ccctcgtccg cctggatgac gccgacgccg
     8521 atctcgacga tttgccgcaa ggcagccgcc tctggagtcg ccggcagaag ccccagatac
     8581 tccgtaagca acccttctga acccaactgc atcgtcgctc caatcggttc cctatggtca
     8641 ctctagggat aggggaggct cgtcgacatc gcaagcgagt gccggcgcgg agatacgacg
     8701 gtttctacat tgaggcaacc gggttttgtc catcagccga caatggcttc gagccaatcc
     8761 ggcctccgga ggacgagaaa accgtcatcg aggtcgagga tgccgtcagc gcgccagatt
     8821 tggatctgct tgttgacggc ctcacgcgac gcatcgatca tggccccgat ctggcgctgg
     8881 gagaggcgca ggttgatccg gatcctttcg ccgtcaggca gcttttcgcc gtgggaatgg
     8941 gccagggcca gaagccgctt ggcgaaccgg gccggcagat tcagaaaagc cgtgtcctcg
     9001 atgatggagc tggtccagcg cacgcgtgcg cagagcaggc cgagcatgtg gatggcgagc
     9061 cgggggtcct cctccaggag tgggatgaag tgctggcggt gcatcgtcag cagttcacaa
     9121 tcttccatcg ccgaagcatt ggccgtgcgg gggcgatcgt cgagcagcgc gatctcgccg
     9181 aacagggcac cgggctcgag aatattgagc aggatttccc gattttccgg gctgttgttg
     9241 aaaatgcgaa cccggccgcg aaggatccca tagagacact cgccgagatc gccctggctg
     9301 aaaatgaccg atccttcgga cacgcttctg accaagctgt aatgcacgat gcgatcgaga
     9361 gtgcggacat cgaggtcctt gagaaggaag tgccgcgaga gcaactcgcg cttgtcgcta
     9421 gcaggtcggg ggatcatgat catcgggcgg aagcgaatgt tccagttgcg gttgcgacaa
     9481 gtatatcagc cccccaccgc gagaccaggc agaatacggc gaaagcatcg tggacgcgcg
     9541 cagaccgcat gcccgagagc aacagcagac gagacgcgtg ccagcgtcgc tctgaaaacc
     9601 attggcgccg aagcgccctc ccccggcgat gccgaagcgc cgtgccggaa tccacgctca
     9661 taatgatccg ggtcaaccga cgcggttgag cgaatacatg cgcgtcgatc caaaaccttt
     9721 cgccatcggc acgttgccaa cgtactcggc gcgcaatcgc ggcgcgtttg ccatcgcgat
     9781 cgccgcggcc atggcctcgg tcacgtagac attccccggc ggcgtgaccg gctcgatccg
     9841 tgcggcgagc gtggtgtgag ccccgaagaa cgtcggctcg tcgcgaatcg gatcgtggcc
     9901 gtcatagacc ggtccaaaat gcgcggagag tcgcagcgcc agctcgggcg acaatccgta
     9961 gccagcggtc ttcgcgcgcg ttatcgcctc ctgaatggcg agacaacatt ccgcggcgac
    10021 gattgggtcg tcgatgacga cgtagatcgc atcgccccag ctgttgcggt acagcacgtg
    10081 ccggccgtag cgacgcaaca cgccggcaat ggtccccatc atgcgcttct gaaacaccgg
    10141 aatgaggtga tcaggagttt tgctgaatcc cttcacgtcg ccgaacaaca gtgcccgcga
    10201 gaagcgcggc ggcatgcggt tcgccggcgt cggacgcttt tgcacgtgtg ggattgcaac
    10261 tgaagactcc gtcaacacca gcacacggct cgccagatct tgcgcatgcc acatccgcag
    10321 atcggaggca tatccggcgg gatcgggatc ccagccgccg atctggagcc tgaggtgcga
    10381 aacctcggta tcaagatgct gcgcgcgcag gatcgccatg cccatagcca gccggcatgc
    10441 atacgtgaat agtgaattgt cgccctgata ggcgtccccg gtcgcaaacg tcacggactt
    10501 ggcacggtcg aggcaagcct tgaagcgtcg cacccagctc gaccctgcga cgcgcaccat
    10561 cgttgcgacg aactcatcct tattgaacgg taagacgacg tgcagctcgg cgccggcccg
    10621 cagacacgcc tcggtgcaga gaatctcgct cccggcgctc aacgaaccgt atgcatatcc
    10681 gatcccgtgc tgcgtgaaat ggtcgacgat cttcttgacg atatcacgct cgcgcgtgcg
    10741 atccggcaaa tgacgcgagg catgaccggc aagcgccggg ccgtaataat gctggactgc
    10801 cggcgggcgc agcagatcga ggatgtcggg actcgtccgg gttgcgcgac atacgagccg
    10861 aagctgcttg cgcgtcgacg cggccgcgtc gaaattcccg ctcaggtacc gtgcggcttg
    10921 aagcaaggcc tggcgcgccc tctcggcgtc tccgcaaatc agcgccgctt ccgcccggct
    10981 cgccaaacga tagtagcgct cgatcgaggt tctgtccgat ccgcgatcgc tcgctctaag
    11041 ggcgcgacga gcgtacaggc gcgcgcgttg ggggtcgccg gccagcaaat agagcgtagc
    11101 cgcgttgacg gccgggtaat gatcgggcgt gcgggcgtaa atccggccat aggccgctgc
    11161 tgcggcgcgc aatgcggtgc gccggtcggc cggcgcggcc agcgcgccgt ccttggcgat
    11221 gcgagcaccg agcgtcgcaa agtcgagctt gcgctcccgc tcgagctcga aacgcttgta
    11281 cagcgctgcc gcctggtcgg tggcgccgct ccgcgccagc actcgggttg caagatagcg
    11341 cagatcgacg ctgtcggggt gctcggccag cccctgcgtc gcaatatcat atgcgacgag
    11401 aaaatcgccg cttcgctcgg cggttctgac cctgtcgatc cagtcggatg gcaacgtgtc
    11461 cgtcatgtcg gttgcgtgcg gcggcggcca cgccgtttcg gttcggcatt tcccgtccta
    11521 ggctgagtga atctagcagc gcggaaggac gataccgcaa tcgcaggcag aagactaaag
    11581 ggagcgcgca agaggatgtc gagcatccgg ccgagattgt ctccaatggg gtcaactcgc
    11641 tttgatgggc ttttgaaccg accaatgcac gttaggcata gcaggccgtt agtcggaaaa
    11701 tcggtggcca cgcaggctcc gatggctttc cgctgaacct cgccacgcca gcaagcgcca
    11761 aaccgccacc gttgaattga acgaaacgag ggttcgcggt cgcgcataca aggccccgcc
    11821 gtaggccaag cctgatgcgt cgggcatctt gattcgcgct ctgaccctcg
//
LOCUS       DASWGM010000428         8088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1710411,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000428 DASWGM010000000
VERSION     DASWGM010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710411"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGA15_12475"
     CDS             complement(<1..507)
                     /locus_tag="VGA15_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9588541.1"
                     /translation="MRYLFEEYAFDTDRRELHRGADVVSVAPQVFDLLDYLIRNRERV
                     VSKADLINVIWNGRIVTDAALTTRLNVARTAIGDSGEEQRLIKTLPRKGFRFVGPVLE
                     AQGPAGGAAPDNPEVPPKPTLTLPDKPSIAVLPFTNLSSDPEQEYFADGMVEDIIAAL
                     SRFKSLFVI"
     gene            670..912
                     /locus_tag="VGA15_12480"
     CDS             670..912
                     /locus_tag="VGA15_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX9588542.1"
                     /translation="MSTTYGATRLGQAAAKRPVYYSPLEKYWDAFLEWRKRQRLRATL
                     CDLSDRELVDIGTTRGEIDYVASNRSVDPRGVRSAG"
     gene            complement(1230..1652)
                     /locus_tag="VGA15_12485"
     CDS             complement(1230..1652)
                     /locus_tag="VGA15_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9588543.1"
                     /translation="MLTRRGFTGFASCAICAVTGFIASEASAQGAPPAVPGVKRTILS
                     QMDGPVPGYVTINMEVEIDPGVTVARHTHPGIESSYVLEGGFELPIQGQPTRMLKAGD
                     GFQIPVETPHAGGKPGDKKSRIAITYVVEKGKPLSSPA"
     gene            1917..2039
                     /locus_tag="VGA15_12490"
                     /pseudo
     CDS             1917..2039
                     /locus_tag="VGA15_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(2052..2228)
                     /locus_tag="VGA15_12495"
     CDS             complement(2052..2228)
                     /locus_tag="VGA15_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588544.1"
                     /translation="MTPHIPEEFSTRLIDWSPFVIIGNTMPPRDPDEDEEDEDEEEED
                     RVDEPPIVREPDED"
     gene            2543..3721
                     /locus_tag="VGA15_12500"
     CDS             2543..3721
                     /locus_tag="VGA15_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588545.1"
                     /translation="MSIEARTTGLRLGAIGIGLLLAAVLGSTAVAADLKIGFTLSLTG
                     GTDDYGKGARMGAELALKEYNDKGGYQSKKVEAVIYDDETKPAKGVENVTRLITRDKV
                     FAMVGPVNSGVALAIIDIAQKNQVPLMDTVATAEQIIERYKNAPKNYIFRVSLNDGIQ
                     TSFMIDYIKGRQYQRVGLMHDSTGWGQSGRDTALRLVKEANLDLVAGPEVFDQNDTDM
                     TTQLTKMQEAKAEFIIAYSLAPAGVQIAKSMQKIGLKTPWTGTWALIAPNFLKLGGKA
                     TVEGVMAVTSYTPDHSANAKVLHEKVDRIFKEQGGDFHPVATAQTYDGVRLLLRALDK
                     VGPDPQKIRDALEEIDDFTEAVTKMKPHPFSHANHESLGRDTGFLAVWHDGKLERASE
                     "
     gene            3878..4765
                     /locus_tag="VGA15_12505"
     CDS             3878..4765
                     /locus_tag="VGA15_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9588546.1"
                     /translation="MSLFFAKLWQALVSGLSIGSIYALIAEGYYITFVTTGALNFGQG
                     EFLMIGALFGLTCYVTFGLPYAVAVIAAVLLTGVMGAALERGAIRPVMRHALSLSWVL
                     STVAVSIILKNAAVNLWGPEQIKFPSPFGEAVLQIGSAGIFPQELFIIVAALGTVFAV
                     QMFLRRSLLGKALIAVAFNRNAAAVMGINVPRMVVFAFVLSSALAGLGGILIAPITFA
                     WAYMGTVPGIKAFAAAIFGGLENPIGILIGGLVIGLLEQLLGMVNSNLKEGITFFFIL
                     IVLAIRPTGLMGRREITKV"
     gene            4767..6581
                     /locus_tag="VGA15_12510"
     CDS             4767..6581
                     /locus_tag="VGA15_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9588547.1"
                     /translation="MQLPIFAFSGPKDWALVALAALILALLPVVFDDPFFILVMQSLA
                     YLFIVTLGLDILVGWTGQISLGHAGLYAVGAYTTALASARLGLPFWLSAPLGVALAGL
                     FGAALAVPSLRAKGPYLAMVTIAFGFMAEVTSNRWSLTGGPMGIMSIPPPTLPDGSEM
                     TATQYFWLIAGVALLCHLLAANLFRSRMGRTLLALQGSEIAAQSVGVNVYRYKVLAFV
                     ISSVYAGIGGVFFAHQNGFINSDTFVFSLSVSLLTSALMGGSGTLFGPLVGSVILNLI
                     PTVFASLHDYHLYIYGGIILVTIVFLPRGIVGSLQQMPALRRLRRPPAVITPDRSVLD
                     FARDSAQDGPVLVVRGLAKSFGGIRALSGVDVAIKRGTVHGLIGPNGSGKSTFVNVVT
                     GVFRASAGKIMLGERELPPQGPHRMAQHGITRTFQSIRLFVDLSVIDNVMVGFHLQLK
                     SGLWAHLLQTRAAVAEEAAYRGKAMALLELVGIAERAHDPAQNLSYGQQRLLEIARAL
                     AARPQLLMLDEPAAGVNPTELHHLSRIIRDIKAAGVTLLVIEHHMELIMGISDVISVF
                     DFGQKISEGSAAAVREDKKVIEAYLGVAEADHDGAVHA"
     gene            6574..7284
                     /locus_tag="VGA15_12515"
     CDS             6574..7284
                     /locus_tag="VGA15_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588548.1"
                     /translation="MLSIEGLKVAYGHVEALRGIDMAMKQGEITAIIGSNGAGKTSAL
                     MAISGLAPVTAGDIRFEGQTITRRRPHDITRLGITHILEGRQLFADQTVEDNLLLGGY
                     TRLPRNRVRVAELMEREMTRFPILRERRRQLAGTLSGGEQQMLAIARGLMSEPRVILM
                     DEPSMGLAPLLVKEIASTIRALRKEGATIVLVEQMAATALRLADHAYVLENGRVKLSG
                     TGAELARMPEVKRAYLGG"
     gene            complement(7463..7576)
                     /locus_tag="VGA15_12520"
                     /pseudo
     CDS             complement(7463..7576)
                     /locus_tag="VGA15_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1504 a         2533 c         2508 g         1543 t
ORIGIN      
        1 gatcacaaac agcgacttga agcgcgatag cgcagcgatg atgtcctcca ccatgccatc
       61 ggcgaaatat tcctgctccg gatcggagct caggttggtg aaggggagta cggcgatcga
      121 aggtttatcg gggagcgtga gggtgggttt cggcggtact tccgggttat cgggggctgc
      181 gccgcccgcg ggcccctgcg cttccagcac gggtccgacg aaacggaagc ccttgcgcgg
      241 caatgtcttg atgaggcgct gttcctcgcc ggaatcgccg atggcagtcc gggcaacatt
      301 caggcgggtc gtcagcgccg catcggtcac gatacgcccg ttccagatga cgttgatgag
      361 atcggctttg ctgacgacgc gctccctgtt gcggatcagg tagtcgagca ggtcgaatac
      421 ctgtggcgcg acggaaacga cgtccgcccc gcgatgcagc tcgcgccggt cagtgtcgaa
      481 tgcgtactcc tcgaagagat agcgcaagct gccaatccct caggcggcct ccgatcatcg
      541 gagctccagc accggttgca acgcaaagaa taagctgtcg gtgaggaaaa tgtaagccga
      601 atgtcaagca cgctcgctcg tgccttggca ccctcgtctg ggtagaagca acacccagga
      661 gattcgacca tgagcacgac ctatggtgca acacggttgg gacaggcggc cgccaagcgg
      721 ccggtctact acagccctct tgagaaatat tgggatgcgt ttctggagtg gcgcaaacgc
      781 cagaggttac gagccacctt gtgcgacctt agtgacaggg agctagtgga catcggtacc
      841 acgcgcggcg aaatcgacta cgtcgcctcg aaccgctctg tcgacccgcg aggcgtccga
      901 tccgctggat gaacgtctga cattgggacc gaatggatgg ccggaattgg acacaaccag
      961 acggaccatt gctcggggtc gacttccggt atgggtcaaa aacggaagtg tccgggctcg
     1021 cgcggcacgt ccgttttacc ctcaggagca gacatcttca gcccgccccg ccatgaccgt
     1081 tttgtgccaa taagcgacat cgcggctgtg atctgaaaaa cgaaagaggc cgccaattga
     1141 ggcggcctcg cctgcagcaa aatggcacac gggccttagg ccagcttccc gagcctgtcg
     1201 tggcgacggc gttggaatcc ttgatctgat caggctggag acgagagcgg tttgcccttc
     1261 tccacaacat aggtgatcgc gatcctcgac ttcttgtcgc cgggcttgcc gccagcatgc
     1321 ggcgtttcaa ccggaatctg gaaaccatcg cccgctttca acatgcgggt cggttgacct
     1381 tgtatgggga gctcaaagcc accctccaag acatagctcg attcgattcc cggatgcgtg
     1441 tgccgcgcaa ccgtcactcc cggatctatt tccacttcca tgtttatcgt cacatagccc
     1501 ggcactgggc cgtccatctg cgagagaatt gttcgcttga cgcctggcac tgcgggcggc
     1561 gcgccctgcg ccgacgcctc cgacgcaatg aaccctgtaa ccgcgcagat cgcacaggac
     1621 gcaaaaccgg taaagcctct tcgtgtcagc atgacgacct ccctaccgtg tccggattgc
     1681 tagtgtccga atttctgaga cgccaataat aggccgcccg tagcggagcg gacaaggtag
     1741 cttgagacga ttgctctggt tttatcgtca ttggccgcgc tgcacaaaaa gcattggcaa
     1801 tgtcctcgct accgaaatgg gtcgtcagcg atatgtccga tttaccccca acagcgaccg
     1861 aaaaacggac attgcgggat gtctcaaacg tgcaaggagc ggaaacattc acagctttgc
     1921 gcaaggccgc cgcgcgctcg gccgaaacca tctgcgtcac aatcggcgaa atcctccgag
     1981 cacttacgcc caccgaatgc gccaactatt tccgaaactc aggctatgcc caatcctaaa
     2041 agcatcatgc tctagtcttc gtccggctcg cgcacgatcg gcggttcgtc gacgcggtcc
     2101 tcttcctctt cgtcctcgtc ctcttcgtct tcatcaggat ctcggggcgg catcgtgttg
     2161 ccaatgatca cgaagggact ccagtcgatc aacctggtgg aaaattcctc ggggatgtgt
     2221 ggggtcatgt tattgctcct cgggtgtgga ccctagggga ttttacccct tctgctccgg
     2281 tttccgcatg agggttagcg ccgcaccgtt tgcggggcat gccagcggtg gtccgcctgc
     2341 ggcggctcat gctttagctt ctggtctgct taccaagtcg gaagttgcgg cgcgcgttga
     2401 agggagacag gaggcagcgt tttctcgtag acgattaccc tgccactgtt tcaatgaggc
     2461 tcaagacgcg gtaaagtcga cagtatggcg tccgccacca gcgacaggat gcccaataac
     2521 gataatccga gggagaacga caatgagcat cgaagcacgg acgaccgggc tgcggctcgg
     2581 cgcaataggc atcggcctcc tgctggcggc cgttctcggc agcaccgcag tcgcggccga
     2641 cctcaagatc ggcttcacct tgtcgctgac cggcggcacc gacgattacg gcaagggcgc
     2701 gcggatgggt gccgagctgg cgctcaagga atataacgac aagggcggct accagagcaa
     2761 gaaggtcgaa gccgtcatct atgacgacga gaccaagccc gcaaaaggcg tcgagaatgt
     2821 cacccgcctc atcacccgcg acaaggtctt cgccatggtc ggaccggtca attccggcgt
     2881 cgcgctcgcc attatcgata ttgcgcaaaa gaaccaggtg ccgctgatgg acaccgtcgc
     2941 cacggcggag cagatcatcg agcgctacaa gaacgcgccg aagaactaca ttttccgcgt
     3001 ctcgcttaac gacggcatcc agacctcgtt catgatcgac tacatcaagg gtcgtcagta
     3061 tcagcgggtc ggcctcatgc acgattcgac cggctggggc cagagcggcc gcgataccgc
     3121 gctgcgcctg gtcaaggagg ccaatctcga cctcgtggcc ggccccgagg tgttcgacca
     3181 gaacgacacc gacatgacga cgcagctcac caagatgcag gaggccaagg cggaattcat
     3241 catcgcctat tcactggcac cggctggcgt gcagatcgcg aaaagcatgc agaagatcgg
     3301 gctgaagacg ccgtggaccg ggacctgggc gctgattgcg ccgaacttcc tcaagctcgg
     3361 cggcaaggcg accgtcgagg gggtgatggc ggtgacgtcc tacacgcccg accacagcgc
     3421 caacgccaag gtgctgcacg aaaaggtcga ccgcattttc aaggaacagg gcggcgactt
     3481 tcatccggtc gccaccgcgc aaacctatga tggggtgcgc ctgctgctcc gcgcgctcga
     3541 caaggtcggt cccgacccgc agaagatccg cgatgcgctc gaggagatcg acgatttcac
     3601 cgaggcggtg accaagatga agccgcatcc cttcagtcac gccaaccacg agtcgctcgg
     3661 tcgcgacacc gggttcctgg cggtgtggca cgacggcaag cttgagcgcg cctccgaata
     3721 acgtgccgag ggttaccgcc ccctgacacg gggaggtcga acagcgaagc tgtccgggag
     3781 cggagaaacc cgtccccgga tcaacttcgg tggtccggcc ctccgtctgc aggagggtgg
     3841 ccaaccacgc gatccgtcgt gccgggggtg tgccgagatg agtctgtttt tcgcaaaact
     3901 gtggcaggcg ctggtcagcg gcctgtcgat tggcagtatc tacgccctca tcgccgaggg
     3961 ctattacatc accttcgtca cgaccggcgc gctcaatttc ggccagggcg agttcctgat
     4021 gatcggggcg ctgttcgggc tgacctgtta cgtgaccttc ggcctgcctt atgcggtcgc
     4081 ggtgatcgcc gcggtactgc tcactggggt gatgggcgct gcgctcgaac gcggcgccat
     4141 ccgcccggta atgcgccacg cgctctccct gagctgggtg ctcagcacgg tcgcggtcag
     4201 catcattctc aagaatgcgg cggtgaatct gtgggggcct gagcagatca aatttccctc
     4261 cccattcggc gaggccgtcc tccagatcgg ctcggcgggc atcttcccgc aggaactgtt
     4321 catcatcgtc gccgcgctcg gcacggtttt cgcggtgcag atgttcctgc gccgctcgct
     4381 gctcggcaag gcgctgattg cggttgcgtt caatcgcaat gcggcggcgg tgatggggat
     4441 caacgtgccg cgcatggtgg tgttcgcgtt cgttctctca tcggcgctcg cagggctcgg
     4501 cggcatcctc atcgctccga tcacctttgc ctgggcctat atggggacgg tgcctggcat
     4561 caaggcgttc gcggcggcga tcttcggggg gctcgagaat ccgatcggca ttctcatcgg
     4621 cggcctggtg atcgggctcc tcgagcaatt gctcgggatg gtcaactcga acctcaagga
     4681 aggcatcacg ttctttttca tcctgatcgt cctcgccatc cgtccgaccg gcctcatggg
     4741 tcgcagggag atcaccaagg tctgacatgc agctgccgat cttcgccttc tcgggtccga
     4801 aagactgggc gctggtcgcc ctggcggcgc tcattctggc actactgccg gtcgtcttcg
     4861 atgatccctt cttcattctg gtgatgcagt cgctcgccta cctgttcatc gtgacgctcg
     4921 ggctcgacat cctggtcggc tggaccgggc agatctcgct cggccatgcc ggactctatg
     4981 cggtcggcgc ctacaccact gcgctcgcct cggcgcggct cggcttgccg ttctggctga
     5041 gcgccccgct cggcgtcgct ctcgccgggc tgttcggcgc cgcgcttgcg gtaccctcgc
     5101 tgcgcgccaa gggcccctat ctcgccatgg tgacgatcgc gttcggcttc atggccgagg
     5161 tgacaagcaa ccgctggagc ctcaccggcg ggccgatggg gatcatgtcg atcccgccgc
     5221 cgacgcttcc tgacggcagc gagatgacgg cgacgcagta tttctggctg atcgccggcg
     5281 tcgcgctcct ctgccatctg ctcgccgcca acctgtttcg ctcccgcatg gggcgcacgc
     5341 tcctggcgct gcagggcagc gagatcgccg cacagtcggt cggcgtcaac gtctaccgct
     5401 ataaggtcct ggccttcgtc ataagctcgg tctacgccgg gatcggcggc gtgttcttcg
     5461 cccatcagaa cggcttcatc aacagcgaca cgttcgtctt ctcgctctcg gtctcgctcc
     5521 tgacatcggc gctgatgggg ggaagcggca cgctgttcgg gccactggtc ggcagcgtca
     5581 ttctcaacct gatcccgacg gtcttcgcca gcctgcacga ctatcacctt tatatttatg
     5641 gcggcatcat cctggtgacg atcgtgttcc tgccgagagg catcgtcggc agcttgcagc
     5701 agatgccggc attgcgtcgc ttgcgtcgcc cgcccgccgt catcacgcca gaccgaagcg
     5761 tactcgattt cgcgcgcgac tcggcgcaag atggcccggt cctggtggtg cgcggattgg
     5821 ccaagagctt cggcggcatt cgcgcgttga gcggcgtcga tgtcgcgatc aagcgcggga
     5881 ccgtgcacgg cctgatcggc ccaaacggct ccggcaagag caccttcgtc aatgtcgtaa
     5941 ccggcgtctt tcgggcgagc gccggcaaga tcatgctcgg cgagagggag ttgccgccgc
     6001 aggggccaca ccgcatggcc cagcacggca tcacgcgcac cttccagagc atccgactgt
     6061 tcgtcgacct ctcggtcatt gacaacgtca tggtcgggtt ccacctgcaa ctcaaaagcg
     6121 gtttgtgggc gcatctcctg cagacccgcg cggccgtcgc cgaggaggcg gcctatcgcg
     6181 gcaaggcgat ggcgctcttg gagctcgtcg ggatcgccga gcgtgcgcac gacccggcgc
     6241 agaacctgtc ctacggccaa caacggctgc tggagatcgc gcgcgcgctc gcggcgcgtc
     6301 cgcaacttct gatgctcgac gagccggcgg ccggggtcaa tccgaccgag ctccatcacc
     6361 tctcgcgcat catccgcgac atcaaggcgg ccggcgtcac gcttctcgtc atcgagcacc
     6421 acatggagct catcatgggg atttccgacg taatctcggt gttcgacttc gggcagaaga
     6481 tctccgaggg cagtgccgcc gcggtgcgcg aggacaagaa ggtgatcgag gcctatctcg
     6541 gcgtcgccga agctgaccac gatggggctg tccatgctta gcatcgaggg tctcaaggtc
     6601 gcgtatggcc atgtcgaggc gctgcgcggc atcgacatgg ccatgaagca aggcgagatc
     6661 acggcgatca tcggctcgaa cggggcggga aagacctccg cgctgatggc gatttccggg
     6721 ctggcaccgg tcaccgctgg cgacatccgc ttcgaaggac aaaccatcac caggcgcagg
     6781 ccgcacgata tcacccggct cggcatcacc cacattctcg aaggccgcca actgttcgct
     6841 gaccagaccg tcgaggacaa cctgctgcta ggcggctaca cccggctgcc gcgcaaccgc
     6901 gtgcgcgtcg ccgagctgat ggagcgcgag atgacgcgtt tcccgatcct gcgtgaacgg
     6961 cgccgccagc ttgcgggcac gctttcgggc ggcgagcagc agatgcttgc gatcgcccga
     7021 ggcctgatgt cggagccgcg cgtcatcctc atggacgagc cgtcgatggg gctcgcgccg
     7081 ttgctggtga aggagatcgc gagcacgatc cgcgcgctgc gcaaggaggg agcgacgatc
     7141 gtcctggtcg agcagatggc cgccaccgcg ctgcggctcg ccgaccacgc ctatgtgctg
     7201 gagaacggcc gcgtcaagct ctccggcacc ggcgccgagc tcgcccgcat gccggaggtc
     7261 aagcgggcgt atcttggggg ctgaaatagt tcgtagccgt gcatagttgt tggtggttcg
     7321 cccatgcgcg cggcgatcct gagtcgtcgc tgctgtggag atgcaagagt ctgcgctttg
     7381 ctgcgaaaat tgtgcgagca aggccacccg atcggccaca cggtggttcg gcatagtcgg
     7441 ggactcggca tagcgcggtc tagacggcgg cggcccgctg cacgccggcg tccgagcccg
     7501 cgaagatcca gttggcccgg ccgagcgcca ccgcgcggac cgcccgttcg gcggcgttgt
     7561 tcgagaggca gatccgcgcg acggctggaa ggagaagtcg aagcccgcca ggtcttgatg
     7621 gtggagagtc gcacgatcgc cgcgtctaca gcccgaagta tttgtgttca gagacgcaaa
     7681 aagacttttg caacactatc ccctcaacaa cggacaagca gtacaatcga cacttgcgaa
     7741 gttgacccgt aatcacgatt gattttgcta cctatatgca gcctagcaat atcgtccaag
     7801 caaacagctc aataagatat tgaaatgaac tgagaacaat ctgcgatgat gaatttccct
     7861 ctccccttgt gggacccctt gtgggagagg ggaagaagga tcgcgccgcg ttcgccctaa
     7921 cccagttcga tgtcgtcggt gatttcgcgg gtgaggctga acgccgccga acaacgcggc
     7981 tgtgtctcag ttgggtcaaa cgctacaggt cgtttgccgc tgggcgggac gcagagtccg
     8041 ctttggccca aaaaccgacc tgattccact gcctaatccg gtggcaca
//
LOCUS       DASWGM010000429         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2403475,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000429 DASWGM010000000
VERSION     DASWGM010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403475"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1350)
                     /locus_tag="VGA15_12525"
     CDS             complement(88..1350)
                     /locus_tag="VGA15_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX9588549.1"
                     /translation="MQSVFNRSVLACAAILTIVGISQAAAQQKPLKKYESGTKEFWTH
                     PPDDWFLGDETEAQKGLAPPSGPPTGSSEAELDAMMKKIKLPQGFKIEVYAPGVLAAR
                     QMAWGDKGTLFVGSFGLGNVYGIKDNGGKKEVKTILKGLNMPTGLAFKDGALYVIAVD
                     KLIKYENAEANLDKVGDGKVVYDDMPSYAAHGWKYLAADKDGWFYIPFGPPFNIGIPP
                     TSVSQIRRVDPKTGNAEVWALGVRNSVGGDVDPRSGKYWFTENARDWISDDLPSDKLN
                     MISKIGEHFGYPYCHQGNLPDTKFAMGHKCSEFTPPVLNLGAHVAPLGMKFYTGSQFP
                     SEYKNSILIAEHGSWNRHKYQGARIVRVTVGPDGKNPKQDVFASGWIEGDQGYLGRPA
                     DIIQDKDGSILVADDWAGAIYRISYSKK"
     gene            1663..2544
                     /locus_tag="VGA15_12530"
     CDS             1663..2544
                     /locus_tag="VGA15_12530"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9588550.1"
                     /translation="MCVLVLGGTGSIGSIVVRELIGCGHDVCGLARSDAAAAKLAEFG
                     ATPVAGDIASPERWAGRLPLVDAVIHAACDFNSDMGAVDRRLLDVLLPSLAAQPKTPR
                     FIYTGGCWLFGATGEDVATEETPLRPLPAFAWMVPHLRRILAAPDIDGIVIHPAMVYE
                     PDGGVFLRFARDAVERDAIRVVESGQVRWPLVHSEDLATLYALALRDAPPGSSYIGAA
                     IEGLAVGRIAGAFARRFGTRRQMPDIISAEALAAEFGAWARGYALDQRLSGAKARRDL
                     RWRPKHLDPEGEIAQLP"
     gene            complement(2632..>3094)
                     /locus_tag="VGA15_12535"
     CDS             complement(2632..>3094)
                     /locus_tag="VGA15_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588551.1"
                     /translation="TDAKKLEKPEWSDKAKDKDGQPIIATATASFEISELCNPTKILL
                     EKGVRYHLEIDAPPDSWSDGGFQVPVGGFSANQPPIWYHRILLGLGVPLRRELTQDWF
                     RIVLRYGRVGGEEVFLDPDPEDSKIEANIRPTRDGELFIFVNDAVIGIPGL"
BASE COUNT          545 a          914 c          969 g          666 t
ORIGIN      
        1 cgaagttgga tcgccacgta ctctaaataa aagggctgcg gctggttcca attgttggag
       61 cagccgcagc ctttgatgat ttacgtctta cttcttgctg tagctgatgc gatagatcgc
      121 accggcccag tcgtcggcga cgaggataga accatccttg tcctggatga tatcggccgg
      181 gcggccgaga tagccctggt cgccttcgat ccagccggat gcgaacacgt cctgcttggg
      241 attcttgccg tccggaccga cggtcacgcg cacgatccga gcgccctgat atttatgccg
      301 gttccatgag ccgtgctcgg cgatcaggat cgagttcttg tactcggagg gaaactggct
      361 tccggtatag aacttcatgc cgagcggagc cacatgcgca ccgagattga gcaccggcgg
      421 ggtgaattcc gaacacttgt gacccatcgc gaactttgta tccggcagat tgccctggtg
      481 gcagtaggga tagccgaagt gctcgccgat cttcgagatc atgttgagct tgtcgctcgg
      541 caggtcatcg ctgatccagt cacgggcgtt ttccgtgaac cagtattttc cgctgcgcgg
      601 atcgacatcg ccgccgacgc tgttgcggac gccgagcgcc cagacttcgg cgttgccggt
      661 cttgggatcg acgcggcgga tctgcgacac gctggtcggc gggatgccga tgttgaaggg
      721 aggtccgaac ggaatataga accagccgtc cttgtcggcg gcgaggtact tccagccgtg
      781 cgcggcgtag gacggcatat cgtcatatac caccttgccg tcgccgacct tgtcgagatt
      841 ggcttcggcg ttctcgtatt tgatcagctt gtcgaccgcg atgacgtaca gcgcgccgtc
      901 cttgaacgcg aggccggtcg gcatgttcag gcccttgagg atggtcttga cctctttctt
      961 gccgccattg tccttgatgc cgtagacgtt gccgaggccg aacgaaccga cgaacagcgt
     1021 gcccttgtcg ccccaggcca tctgccgcgc ggccagcacg cccggcgcgt agacttcgat
     1081 cttgaacccc tgcggcagct tgatcttctt catcatcgcg tcgagttcgg cttcggaaga
     1141 gccggtcggc ggacccgatg gcggtgcgag gcccttttgg gcttcagtct cgtcgccgag
     1201 gaaccagtcg tcgggcgggt gggtccagaa ttccttggtg ccggactcgt attttttcag
     1261 gggtttttgc tgtgcggcag cttgtgaaat ccccacaatg gtgaggatcg cggcgcacgc
     1321 aagcaccgat cgattgaata ccgattgcat tacgtcactc cctaagcagc ccatgcggct
     1381 gcgcttttat ttttgaacgt ttcgagatga actttggtgg gtttgcagtg aaggtgaggt
     1441 ggatcagacc tcaaaacgtt atcacatccg tcgcaggaga gaagcgccgc cgcgcggacc
     1501 tgtcaggcga aaacaaatgg agaagaaatt tcttcaggcc gtgagattgc ggtaatttca
     1561 agcaggcgtc cgctgcactg caatatgtcg ggacgttgca atcgtgcggt cgccggatca
     1621 ggcaagcatc acgttgcaag catcacgtta aggagccggc tcgtgtgcgt tttggttttg
     1681 ggtgggacgg gttcgatcgg ctccatcgtg gtgcgagagc tcatcgggtg cggacatgat
     1741 gtctgcggtc tggcgcgatc cgacgccgct gcggcgaagc ttgccgaatt tggcgcgacg
     1801 ccggtcgccg gcgatatcgc gtcgcccgag cgatgggccg ggaggcttcc acttgtcgat
     1861 gccgttatcc atgccgcctg cgatttcaac agcgatatgg gcgcggtcga tcgtcgcctg
     1921 ctcgacgtac tgctgccatc cctcgccgcg cagccgaaaa cgccacgctt catctatacc
     1981 ggcggctgct ggctgttcgg cgcgaccggc gaagatgtcg caaccgagga gacgccgctt
     2041 cgtcccctgc ctgctttcgc gtggatggtg ccccatctgc gccgaatcct cgccgccccc
     2101 gacatcgacg gcatcgtcat ccacccggcg atggtctacg agcccgacgg cggcgtcttc
     2161 cttcgctttg cccgggatgc cgtcgagcgc gatgcgatcc gggtggtgga aagcgggcag
     2221 gtacgctggc cgctcgtgca cagcgaagac ctcgcgaccc tctacgctct ggcgcttcgc
     2281 gacgcgccac ctgggtcgag ctatatcggc gccgcgatcg aaggacttgc cgtcggccgc
     2341 atcgccggcg cttttgcaag acgtttcggc acgcggcgcc agatgccgga cattatttcg
     2401 gccgaagcgc tggcggccga attcggcgct tgggccaggg gttatgcgct cgaccagcgg
     2461 ctgagcggcg cgaaagcccg gcgcgatctc cgctggcggc cgaagcatct cgatccggaa
     2521 ggtgaaatcg cgcagctgcc gtgatggaga acggatcggg cccgcgtccg ctaatatttc
     2581 cgcgtcagcg tcagctttcc gccaccgcca ttgttgcgat agaaaaaatc gttagagccc
     2641 gggaatgccg atgaccgcgt cgttgacgaa gatgaaaagc tcgccgtcgc gcgtgggcct
     2701 gatgttggcc tcgatcttcg agtcctcggg atcgggatcg agaaacacct cctcgccacc
     2761 aacacggcca tagcgcagca cgatccggaa ccagtcctgc gtcagttctc gtcgcagcgg
     2821 cacgccaagt cccagtaaaa tccgatgata ccagatcgga ggttggttgg cggagaatcc
     2881 gccaaccgga acctgaaagc caccatcgct ccaggagtcc ggcggcgcgt cgatctccag
     2941 atggtatcgc acgcccttct caagaaggat tttcgtcgga ttgcagagct ccgagatctc
     3001 gaaggatgcg gttgcggtcg cgatgatcgg ttggccatct ttgtctttgg ctttgtctga
     3061 ccattcgggc ttttcgagtt ttttggcgtc ggtg
//
LOCUS       DASWGM010000430         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1839642,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000430 DASWGM010000000
VERSION     DASWGM010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839642"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1727
                     /locus_tag="VGA15_12540"
     CDS             333..1727
                     /locus_tag="VGA15_12540"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9588552.1"
                     /translation="MNPDTLAQFSKEELIALILAQAEVIARQTAQIEALTKQIDVLMK
                     RVEALEAELGKPPKTPDNSSVPPSQGQKPNRAERRAGKRRKGHPGAFRQLSETPDRVV
                     ETLAAACPHCDHVLCAADQSEFHAYDHIDLPPIKPVVTRVHRHRGVCPCCRRGFSAPF
                     PEGMAPGSPFGPNLAALILHLHVTQAISFERLVRLMDEVFGVTISEGAIANRLARAEA
                     PMIIAAEKIAEEVRSSPVVASDETSARVKGKTWWQWVLLSSTAVYHVIADSRGAKVVI
                     DFLQGAVPEVWVADRYAGQNGHGIERQLCLAHLLRDSQYAIDEGDTAFAPGFKHLLLR
                     AVAIGRRRETLKDSTLVQYRADLDRRLTRLLGQPPTTSAGRKLARAIRKCRNDLFIFI
                     TRRDVPYTNNGCERALRPSVIFRKVTGSFRSYWGARLYAAAASVIATGRLHGKSALRA
                     IQDVIAAQPAAVIP"
BASE COUNT          362 a          625 c          584 g          346 t
ORIGIN      
        1 cggaagatgc catctgcgcg cgccgcaaac gcattcctaa atgcggttcg caagatacga
       61 tggacagtcg ctggaaaaga aacatcgtcc atgtcattga cggtcaatcc accggcaaaa
      121 tcacctaatt gttcctgcat gtagtcaagc cgctgttgtc gattctgctt aagtagtacg
      181 ccgggcggct cagcgtatac tgtagcaaac gcaaacccgc cgacctttaa tttgtaactg
      241 ctcaccctgg gaggtagcag ggtttgtgag cgagtggcgg caggccacaa aatgtagttg
      301 acagatgtca gtgtctgcga ttcaaggatt cgatgaatcc ggacaccctg gctcagttct
      361 cgaaagaaga gttgatcgcg ctgatcctgg cgcaggcgga ggtgatcgcg cggcagactg
      421 cgcagatcga ggcactgacg aaacagatcg acgtgctgat gaagcgggtc gaggcgttgg
      481 aggcggaatt ggggaagcct cccaagacgc cggacaattc ctcggttccg ccgagccagg
      541 ggcagaagcc caatcgggcc gagcggcgcg caggcaagag gcgcaagggt catcccggcg
      601 cgttccggca gctgagcgag acccccgacc gcgtggtgga aacgctggcg gcggcatgcc
      661 cgcattgcga tcacgtgctg tgcgccgccg accagagcga gtttcatgcc tacgaccata
      721 tcgacctgcc gccgatcaag ccggtggtga cccgcgttca ccggcatcgc ggcgtctgcc
      781 cgtgctgtcg ccggggcttc agcgcaccct tccccgaagg catggcgccg ggctcgccct
      841 tcggccccaa ccttgcggcg ctgatcctgc atctgcacgt gacccaggcg atcagcttcg
      901 agcgcctggt gcggctgatg gacgaggtgt tcggtgtcac catcagcgaa ggcgccatcg
      961 ccaatcggct ggcccgcgcc gaagcgccga tgatcatcgc ggccgagaaa atcgccgagg
     1021 aagtccgctc gagcccggtc gtggcctcgg acgagacctc tgcccgggtc aagggcaaga
     1081 cctggtggca atgggtgctg ttgtcgtcga cggcggtcta tcacgtgatc gccgacagcc
     1141 ggggcgccaa ggtagtgatc gacttcctcc agggtgccgt gcccgaagtc tgggttgccg
     1201 accgctatgc cggtcagaac gggcatggca ttgagcgcca gctctgcctc gcccatctgc
     1261 tgcgcgacag ccaatatgca atcgacgagg gcgacaccgc cttcgccccc ggcttcaagc
     1321 atctcctcct gcgcgccgtc gccatcggcc ggcggcgcga gaccctcaag gacagcacgc
     1381 tcgttcaata tcgggccgat ctcgatcgcc gcctcaccag gctgctgggc cagcctccga
     1441 ccacgtcggc cggccgaaag ctcgcccgcg ccatccggaa atgccggaac gatcttttca
     1501 tcttcatcac cagacgcgat gtgccctaca ccaataacgg ctgcgagcga gccctgcggc
     1561 cctccgtcat cttccgcaag gtcaccgggt ccttccgatc ctattggggt gcccggctct
     1621 atgccgccgc ggcctccgtc atcgccacgg gacggctcca cggaaaatcc gccctccggg
     1681 ccatccagga cgttatcgcc gcacagccgg cagcggtcat cccgtgaccc gcacgtgagc
     1741 agttacaatt caaggcgctg acgaaatacg ttcagcaggt aataccgctt ttcgcgcgca
     1801 ccccaggtcg tgcatacgct gtaatcgctg agctcggtcg ccttgtttgc ggtatcccag
     1861 ctctgcacgg tctgactgaa ccgctcaggg cgttcactcg gctcgtagta ttgcagc
//
LOCUS       DASWGM010000431         6943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2403738,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000431 DASWGM010000000
VERSION     DASWGM010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2403738"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VGA15_12545"
     CDS             complement(<1..467)
                     /locus_tag="VGA15_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9588553.1"
                     /translation="MPLTDQLRDDILASVPSLRAFAISLSGNGDRADDLVQETLLRAL
                     ANIDSFQPGSNLPAWLFTILRNLFRSDYRKRRREVEDADGSYAKTLKTQPAQNAHLEF
                     EEFRAALEKLPQDQREALILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLS"
     gene            complement(467..673)
                     /locus_tag="VGA15_12550"
     CDS             complement(467..673)
                     /locus_tag="VGA15_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEX9588554.1"
                     /translation="MKETKKPAKPGGLNAEIQSRIGHQLRAMYDDVVRQGVPDRFAEL
                     IRKLDTQEANPQLGTAGEKTEGSD"
     gene            964..1770
                     /locus_tag="VGA15_12555"
     CDS             964..1770
                     /locus_tag="VGA15_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9588555.1"
                     /translation="MSRSQLVAEHLPLLRRYARALTGNQASGDAYVGAMLEALLQDPA
                     LLDEAHGPRAGLFRLFTQIWNSVSLNDDTDADVATLPMPSERRLSNITPLPRQAFLLL
                     SLEGFSEEEVAFVLGIDVAETRQLADTAGREMAAEIATDVLIIEDETFIAMDLESLVK
                     NLGHNVIGVARTHSDAVALAKNRKPGLILADIQLADGSSGLDAVNELLGIFEVPVVFI
                     TAYPERFLTGERPEPAFLISKPFQPAMVSAVASQALFFQRNSRNRAPRVA"
     gene            1978..2322
                     /locus_tag="VGA15_12560"
     CDS             1978..2322
                     /locus_tag="VGA15_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588556.1"
                     /translation="MDYSIPQDWIAEAGLDGFMPATQGYRCDEPHSLVALTDIAPIKR
                     DRPLDRNGFVRDRMMAVLRGISKGESIPPIIIWAALAGPPRFELRDGYHRLYASATFG
                     FSHVPADIIDRY"
     gene            complement(2319..3086)
                     /locus_tag="VGA15_12565"
     CDS             complement(2319..3086)
                     /locus_tag="VGA15_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEX9588557.1"
                     /translation="MMESLHVFIDESGVHDDSPVVTVAMYAGQPTDWRLFVKNWNAAK
                     KPIKIIHAADCANRTGEFEGWDRDRRNAFAAQLLPVLPSHNIVGVAVGINLEHFRAAM
                     AAHPELAEMVGTPYSACFQWAVQTFLNVMGRYGYDQPVHFFHEHNDYEDEARIAFDFV
                     QAHRPTKEPIALSFIGKADAVELQAADVLAYEANHYLRSPKGVRRPSWHAMDPNRDII
                     TLLHYGENNMQNLVSTLLDFRRRALAAGWDGIVDATR"
     gene            3746..4141
                     /locus_tag="VGA15_12570"
     CDS             3746..4141
                     /locus_tag="VGA15_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588558.1"
                     /translation="MSQLSRAVFGTIAVSLTLGAVQLAAGRDLTGTQNPAGNRAVKAD
                     RADAAPGSRTATRTISLRLDALADTSVVIRMPAAKATDKQATDKQARGSSAPLLTKSG
                     DRKATVACEPPVSVLTEVAKRLQPGRCIT"
     gene            complement(5204..5638)
                     /locus_tag="VGA15_12575"
     CDS             complement(5204..5638)
                     /locus_tag="VGA15_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588559.1"
                     /translation="MFARMIDDIKDQTGTALRLTSLAAAVAFALFVTTSFLCAAAFVF
                     MLQKYGLIEACLTGAGIFFVVTLIAAGCYMVRKNRVKARVEETAKSAMHTALADPMLV
                     AAGIQVIRAIGIKKLIPILAVGGLALGFLASRNSSVDEAPAE"
     gene            complement(5808..6179)
                     /locus_tag="VGA15_12580"
     CDS             complement(5808..6179)
                     /locus_tag="VGA15_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HEX9588560.1"
                     /translation="MSSTDGEIGMRNMTDKATYERLEKDVTAVKNDISALTDQITDAL
                     NSFAGTAQKQARRGYKQARANVDSAVDDMSERGSAMMDAAQDAATSIEESLEEVITQR
                     PLATVGLALGLGFLIGATWRR"
     gene            complement(6256..>6943)
                     /locus_tag="VGA15_12585"
     CDS             complement(6256..>6943)
                     /locus_tag="VGA15_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX9588561.1"
                     /translation="LQSMLGGSDTLSTFQMPKFDWVQPAVEFLSPTFTEFLLFFATLI
                     LFLASWRDLRRALIMTFADHAARLRMLRILNEIEGHLGGYLITVTMINLGVGAATGII
                     CAITGMPNAAGLGALAATLNFFPIIGPVAMFAILTVVGVIAFSTIGGGLIAPLAFIGV
                     VFIEGHFVTPTIIGRRLELNALAVFMALAFWTWLWGPMGGFLSSPLLIVALVLKDHLM
                     PADSPQLPPD"
BASE COUNT         1360 a         2094 c         2104 g         1385 t
ORIGIN      
        1 gagagcttcg atcgcgcccg gttgacacgg ctcttgatgg tgccgaccgc gcagccgcag
       61 atcgccgcgg catcttcata ggagaagccc gaggcgccga ccagaatcag cgcttcgcgc
      121 tggtcctgcg gaagtttttc cagcgccgcg cgaaactcct cgaactcgag atgcgcgttc
      181 tgcgccggct gggttttcag cgtcttggca tagctgccat cggcgtcctc cacctcccgt
      241 cgccgcttgc gatagtcgga gcggaacagg ttgcgaagga tcgtgaacag ccatgccggc
      301 aggttggagc cgggctgaaa cgagtcgatg ttggcaagcg cgcgcagcag ggtttcctgc
      361 acgaggtcgt cggcccggtc gccattgccg ctgagcgaga tcgcgaacgc ccgcaggctt
      421 ggaaccgaag ccaggatgtc gtcgcggagt tgatctgtga gaggcattag tcgctcccct
      481 cggttttttc gcccgcagtt cccagttggg gattggcctc ctgcgtatca agctttcgga
      541 tcagttccgc gaaccggtcg ggaactcctt gccgcaccac atcgtcgtac atggcgcgca
      601 gctggtggcc gattctcgat tgaatctcgg catttaggcc gcccggcttg gctggctttt
      661 ttgtttcttt catgagctgt tccacgcttc cccgagagtt aagtccctga aacttcaata
      721 attatgcttg atttggaaaa tctttgagac ctctctatgg aaacctaatg caaagtccgc
      781 cggatagttc cggaacgcgg gaacttttct catgttcggg cgtagtcagc caccagggga
      841 accgggtcac cccatcttga tggtttggcc caaatgactg accgagaacc ggcccagaaa
      901 ctgatcagga accgattcgg gaactgatcc aaggcttaaa ctcaggaaaa tgatggagtg
      961 gggatgtccc gatcacagct cgttgccgaa catttgccgt tgttacggcg ctacgcccgt
     1021 gcgttgaccg gaaaccaggc gtccggcgac gcctatgtcg gtgccatgct tgaagccctg
     1081 cttcaggatc ctgctttgct cgacgaggcg catggcccgc gcgccgggct gttccggctg
     1141 tttacccaaa tctggaattc ggtctcgctc aacgacgata ccgacgccga cgtggcgacg
     1201 ctgccgatgc cttcggagcg ccggctttcc aatatcaccc cgttgccgcg ccaggccttc
     1261 ctgctgctgt cgctggaagg tttttcggaa gaggaagtgg cgttcgttct cggcatcgat
     1321 gtcgccgaga cccggcagct cgccgacacc gccggccgcg aaatggcggc ggagatcgcc
     1381 accgacgtgc tgatcatcga ggacgagact ttcatcgcga tggatctcga gagcctggtg
     1441 aagaacctcg gtcacaacgt gatcggcgtc gcccgcaccc actccgatgc ggtggcgctg
     1501 gccaagaaca ggaagcccgg cctgatcctg gccgatatcc agctcgccga cggcagctcc
     1561 ggcctggatg ccgtcaacga gctgttaggg atcttcgagg tgccggtggt cttcatcacc
     1621 gcctatcccg aacgcttcct gaccggcgaa cgcccggaac cggcgttcct gatctcaaaa
     1681 ccgttccagc ccgcgatggt atccgccgtc gccagccagg cgctgttctt ccagcgcaac
     1741 tcgcgcaacc gcgcgccaag ggtggcgtag gggcggctcg ctgaagcgag gctcgttgaa
     1801 ttgatccggc gcgccgaagg gcgcgcctga ttttttgtga gtgcggacgc tcgaaatgtt
     1861 cgccaatcaa tttgtgaatt tttgccagca attgaatagc gaccccgaac agcgacttaa
     1921 agagtggact gcgtgcgttc acgacaagca gccgtgcaaa ggaccgtagc ctatctgatg
     1981 gattattcga ttccgcagga ttggattgca gaagcggggc ttgatggttt catgccagcg
     2041 acgcaaggct atcgctgtga cgaacctcat agcttagtgg cgctgacgga tatcgcccct
     2101 atcaagcgcg acaggccgct agatcggaat ggttttgttc gcgacaggat gatggctgtt
     2161 ttgcgcggca tcagcaaggg cgagtcaatc ccacccataa tcatttgggc cgcgctggcg
     2221 gggccgcccc gcttcgagct aagggacgga tatcatcgcc tctatgcatc agctacgttc
     2281 ggattctctc acgtcccggc ggatatcatc gaccgctact agcgagtcgc atcgacgatg
     2341 ccatcccaac cggcggccaa agcacgtcgc cggaaatcca gcaacgtcga aacaagattc
     2401 tgcatgttgt tttcgccgta atgcagtagc gtgatgatgt cgcggttcgg gtccattgca
     2461 tgccacgatg gacggcgcac gcccttcggc gaccgcagat agtggttagc ctcataggcc
     2521 agcacgtcag ccgcttgcaa ttcgacggcg tcggccttgc cgatgaacga cagcgctatc
     2581 ggttctttcg tcggacgatg cgcttgaaca aaatcgaacg cgatgcgcgc ttcgtcttca
     2641 tagtcgttgt gttcatggaa gaaatggacg ggctggtcat agccgtatcg gcccatgaca
     2701 tttaggaacg tctgcaccgc ccattgaaag caagcggaat agggcgttcc gaccatttcg
     2761 gccaactcag gatgcgcagc catcgcggcg cggaaatgct ctaggttgat tcctacagcg
     2821 acgccgacaa tgttatggct cggcaacacc ggcagcaatt gcgcggcgaa cgcgttgcgc
     2881 cggtccctat cccatccttc gaattcgcca gtccggttag cgcagtcggc ggcatgaatg
     2941 attttgatgg gtttcttcgc tgcgttccag ttcttgacga acaagcgcca gtccgtcggt
     3001 tgcccggcgt acatagcgac cgtgacgacg ggcgaatcat cgtgaacgcc ggactcgtcg
     3061 ataaagacgt gaagcgattc catcatgccg aacagtatag ccgaatcggc aacaagaagc
     3121 ccggcgcgat ggccgggctt tgtcgcgtcg tcgatgggtc gccggccaag gccgcgtcat
     3181 tgcgcagttg gtcacgtcgt ccgggccgga ttagcgaaag cgaatcatgc cttcggaagc
     3241 ctgccagcga atcggatggc accgtaattc tcgatggcat tgcgagcgag ccagcggacg
     3301 aaggtcagat agccgggcac gttccggctg ttatcgatat atgcggcgtc ggcgatgtac
     3361 ggcgcgccgc cagatcaccg gcgtcaatcc aattggggca atcaaacaat gtgagcatgg
     3421 gaatcacttt tttgcggtgg tgataccatg gtctggcggg ctggtgtatg ggcgaaaggt
     3481 gtgtggctta aactaaccat ctaatattat tataataaaa gcagggcgcg accggcatca
     3541 ccacagtcgc gccctacgtc gccggtcaat ggggcagggg ggatgaccgg ctgtcggaat
     3601 gactcccaga cccccggact cgttccggtc cccagatatt tttttaccac acgcttaatt
     3661 gatttcggcg cgttgaaccc aagcccgtct gtcgcattct aactactaac cgccattggg
     3721 gcgacggacg acgggcaggc gtgacatgtc acagctatcc agggcagttt tcgggaccat
     3781 cgccgtttcc ctcaccttgg gcgctgttca gctggcggcg ggccgcgatc tcaccggaac
     3841 tcagaatcca gcgggcaacc gtgccgtcaa ggctgatcgc gccgatgccg cgccgggttc
     3901 gcggactgcg acgcgaacga tttcgctccg gctcgatgcg cttgccgata cctccgtcgt
     3961 catccgcatg ccggccgcca aggcgaccga caagcaggcg accgacaagc aggcccgagg
     4021 ttcatccgca ccgttgctga caaagtccgg ggatcggaag gcgacggtgg cctgcgagcc
     4081 cccggtcagc gtgttgaccg aagtcgccaa acggcttcag cccggccgct gcatcacctg
     4141 agagaatttt cccttttcgc cgtcgccggg ctttgttctg ccctgccggt tctggtcgtt
     4201 ccccggatgc tgcgcagcgc cttcagcgcg tttacgcgcg tcttcgacgc gccatcagcg
     4261 cgtttacgcg cgtcttcgac gtgccatcag cgcgtttgcg cgcgtcttcg acgcgccatc
     4321 agcgcgttta cgcgcgtctt cgacgcgcca tcagcgcgtt tacgcgcgtc ttcgacgcgc
     4381 tatggcagcg gtgcgctgct aatccggggt ccatcgtacc gctggtctgc tatgggtccc
     4441 ggctctgcgg agcagcgtga agtacgctgc accgcgtccg ggacacgagc ggaatttgct
     4501 ctcgctgccg tgtgcggatt tgcgcgcacc ggcgaatttg ttgaattgga tcaatctgat
     4561 ttgggcagtc cagtcctctt tgcaaaaata tttctgtttc cgtacccccc aaatcacatc
     4621 tagaactttt cgcatcccgt cccacacaga ggggcgtatc atgatcgtca cgaacgtagg
     4681 ggcgggatgc ggtggacgcg gcagcgtttt gcgcgcgatg ggatcgcagg gcgggttctt
     4741 tgaatccgtg agcgatcaaa gagcgggcgg acgagagatg ttgctgcgga cggcaaaatc
     4801 gtgtggtcct gacgccccga cgctggcgtc aagttttgcg gatggcaagt cggcccaacc
     4861 gggcgcagac aagccagata tccgctggcg acggtggcaa aagagcccgg tcaccgggga
     4921 gagcacgaca taagccgtaa aaccattgcg tgcgggaatg ccgggtgttc cggtgtactc
     4981 gttgttactc gtgtgctttc taccaataca aagtgcacac gaggccgcgg gtgcagcggg
     5041 cacccggcat tccccacgcc ctccttgggg cggaagattc atcaaagact cgggcgcatc
     5101 gcgccgcgag atcgcgaagc tacgtttgcg aacagtttgg ctgtttgaaa attgaattgc
     5161 gcactaggcg gggacgacgc tgagagatta cgcgccgccc acatcactcc gcaggcgctt
     5221 catcaaccga actgttgcgg ctcgccaaga agcccagcgc caatccgccg accgcgagaa
     5281 tcgggatcag cttcttgatg ccgatggcgc ggatcacctg gatgccggcg gcgaccagca
     5341 tgggatcggc cagcgcggtg tgcatcgccg acttcgcggt ctcctcgacg cgggctttga
     5401 cccggttctt gcgcaccatg tagcagccgg ccgcgatcag ggtgacgaca aagaaaatgc
     5461 cggcgccggt caggcaggcc tcgatcagtc cgtatttctg cagcatgaac acaaaggccg
     5521 cggcgcagag aaacgatgtc gtgacgaaca aggcgaacgc caccgccgcc gccaaggatg
     5581 tcagccgcaa ggccgtgccg gtctggtcct tgatgtcatc gatcattcgc gcaaacatct
     5641 gaaagcctcg ttcaatatgt cgacgaaaca actgacgccg cctctcgcgc cgccattcaa
     5701 atcaaaacaa ctgatcccgc cgcggcggcg aacgcgcgcc aggtgcctgt cgtcagccgg
     5761 tcgttatctg cgccaagtta tctgcaccaa atcacctgcg ccaaatctca cctgcgccaa
     5821 gtcgcgccga tcaggaagcc gaggccgagc gccagcccaa cggtcgccag cggccgctgc
     5881 gtaatgacct cttccagcga ctcttcgatc gaagtggccg cgtcctgggc ggcatccatc
     5941 atggcgctgc cgcgctccga catatcgtcg accgccgaat cgacattggc gcgggcctgc
     6001 ttgtagccgc gccgtgcctg cttctgggcg gtaccggcga atgaattgag cgcgtcggtg
     6061 atctggtcgg tcagggcaga gatatcgttt ttcacggctg ttacatcctt ctcgaggcgt
     6121 tcataggtcg ctttgtccgt catgtttcgc attccgatct cgccatctgt gctcgacatc
     6181 aaaagctccc ggggtactga ggttagctac ggcacaaacg ccccgcttcg cggtaagttc
     6241 cgcgaaacga accgatcagt ccggcggcaa ttgcggcgaa tccgccggca tcagatgatc
     6301 tttgagaacc agggccacga tcaaaagcgg cgaggacagg aagccgccca tcggtcccca
     6361 cagccaggtc cagaacgcca gcgccatgaa cacggcgagc gcgttcagct cgagccggcg
     6421 gccgatgatg gtcggtgtga caaaatgacc ctcgatgaaa acgactccga taaaagccag
     6481 cggcgcgatc aggccgccgc cgatggtcga gaaggcgatc acgccgacca cggtcaggat
     6541 cgcgaacatc gctaccgggc cgatgatcgg aaagaagttc agggtcgcgg ccagcgcgcc
     6601 gagcccggcc gcgttgggca ttccggtgat cgcgcagatg atgccggtcg cggcaccgac
     6661 gccgagattg atcatggtca cggtgatcag gtagccgccg agatgtccct cgatttcgtt
     6721 gagaatccgc agcatccgca accgcgcggc gtgatcggcg aacgtcatga tcagcgcgcg
     6781 gcgcagatct ctccagctcg ccaggaacag gatcagcgtg gcgaagaaca gcaggaattc
     6841 ggtgaaggtc ggcgacagga attccacggc cggctggacc cagtcgaatt tcggcatctg
     6901 gaatgtcgag agcgtgtcgg atccgccgag catgctctgc agt
//
LOCUS       DASWGM010000432         6078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_48377,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000432 DASWGM010000000
VERSION     DASWGM010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48377"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VGA15_12590"
     CDS             <1..674
                     /locus_tag="VGA15_12590"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9588562.1"
                     /translation="ASMDGGAAGVMIAPPPHLRTDDQITGYFKQAIEAIGDDIPWVLQ
                     DYPLTLTVVFTPAVIRKIVMDNPSCVMLKHEDWPGLEKISALRAYQKESSLRPLSILT
                     GNGGLFLDFEMERGADGAMTGYAFPELLIDVVKLSKAGKRDAAHDLFDAHLPLIRYEQ
                     QPGAGLAVRKYVLQKRGVIASSAQRKPGSSITATAKAEVDYLLSRVARVDKRANLQPQ
                     SSAAE"
     gene            679..1473
                     /locus_tag="VGA15_12595"
     CDS             679..1473
                     /locus_tag="VGA15_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9588563.1"
                     /translation="MPEAVTPRPASTILLLRDSAASEIEVFMMVRHYEIDFSSGALVF
                     PGGSVDKGDQDIIASPELYSGGEGLDTASLSFRIAAIRETFEESGILLARPRESKALV
                     DARRASAIEAAHRTALCEGKTTFLKVLTDSGMLLALDELVPYAHWITPEGLPKRFDTW
                     FFLAAAPPEQVGAHDGKESTDSIWVSPREALEGGKSGRFKLPFPTTRNLIRLGKQPNV
                     KAALDDSIGKPIVTVMPVVTKRDGVRQLRIPLEAGYDGEVFEVGAA"
     gene            complement(1475..3034)
                     /locus_tag="VGA15_12600"
     CDS             complement(1475..3034)
                     /locus_tag="VGA15_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9588564.1"
                     /translation="MNVTQGLRRVLQTNPEGIATVDGDRRRSWREIGERVARLAGGLR
                     ELGVNRGDRVAVLMLNADRYLELYLGIAWAGAVIVPTNIRWSHAEIEDSLHDCRASVL
                     VVDKAFAKMGVELAKAVPLALIYADDDSGPTEAQNYETLITVSQPVPDAMSGREELAG
                     IFYTGGTTGRSKGVMLSHANIVSNALHMLSEGLLPDGTIYLNAAPMFHLANGGAMFAS
                     LIGGGTNVIVRTFNPELVMDTIEREKVTATLIVPTMIQMLVDHPAFQTADLSSLQRIM
                     YGASPISEALLNRAMWGLPGTAFHQLYGMTELSPLATHLPWDQHTGEAASKKNRQRAC
                     GRAAIGCEVRIVDADRKPVASGVVGEVAVRGQNVMMGYWERPEETAKAVIDGWMHTGD
                     GGYMDEEGYVYLVDRVKDMIISGGENIYSMEVENAIAQHPAVSQCAVIGIPSEQWGET
                     VHAFVIPKAGAHVNAAELIAFCKERIAGYKCPRSVDVRTEPFPLSGAGKVLKRELRRL
                     YGQDRAQAKAG"
     gene            complement(3518..4195)
                     /locus_tag="VGA15_12605"
     CDS             complement(3518..4195)
                     /locus_tag="VGA15_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9588565.1"
                     /translation="MVRAGKAVFDARVFLAGVGAGKTKLEFQKNQNVFAQGDVADTVF
                     YIQKGKVKVTVLSDQGKEAVVGILGPGQFLGEGSINGHKFHISTATAMVDCVITAITK
                     DAMLAALHDEPKLSEMFMAHLLTRNGRIEEDLIDQLFNSSERRLARMLLLLANFEKAG
                     SPQPISPNISQETLAEMIGTTRSRVSHFMNKFRKLGLIDYNGRIEVHNSLLSAVLHEK
                     PRLKEDH"
     gene            complement(5076..5804)
                     /locus_tag="VGA15_12610"
     CDS             complement(5076..5804)
                     /locus_tag="VGA15_12610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9588566.1"
                     /translation="MARELEGKVAVVTGAASGIGLASTEAMLAAGSRVVMVDRDEAAL
                     KVLCNKHGDAVIPLVIDLLDPKDCATLLPRVLEKVGQLDILHANAGTYVGGDLVDADT
                     GAIDRMPNLNVNVVMKNVHDVLPHMIERRTGDIIVTSSLAAHFPTPWEPVYASSKWAI
                     NCFVQTVRRQVFKYGIRVGSISPGPVISALLADWPPEKLKEAKESGSLQEASEVANVV
                     TFMLTRPRGMTIRDVVMLPTNFDL"
BASE COUNT         1166 a         1837 c         1870 g         1205 t
ORIGIN      
        1 gcgcctcgat ggacgggggt gcggccgggg tgatgatcgc gccgccgccg catctgcgca
       61 ccgacgatca gatcacgggc tatttcaagc aggccattga agcgatcggt gacgatattc
      121 cctgggtgct gcaggattat ccgctgacgc tgacggtggt gttcacgccc gcggtgatcc
      181 gcaagatcgt gatggacaat ccctcctgcg tgatgctcaa gcacgaggac tggccggggc
      241 tggaaaagat ttccgcgctg cgtgcctacc aaaaagaaag ctcgctgcgg ccgctgtcga
      301 tcctgaccgg caatggcggg ttgtttctgg atttcgagat ggaacgcggc gccgacggcg
      361 ccatgacggg ctatgccttc ccggaactgc tgatcgacgt ggtcaaactc tcgaaagccg
      421 gcaagcgcga cgccgcgcac gatctgttcg acgcccatct gccgctgatc cgctacgagc
      481 agcagcccgg cgcgggcttg gcagtgcgca aatacgtgct gcaaaaacgc ggtgtgatcg
      541 cctccagcgc gcagcgcaag ccgggttcga gcattacggc aactgcaaag gccgaggtgg
      601 attatctgct gtcgcgggtc gcgcgggtcg acaagcgcgc gaatttgcag ccgcaatcca
      661 gcgcggcgga gtgatttgat gcccgaagcc gtaacgccgc ggcctgcgtc caccatcctg
      721 ctgctgcgcg acagcgccgc cagcgagatc gaggtcttca tgatggtgcg ccattatgag
      781 atcgacttta gttccggcgc gctggtgttt cccggcggca gtgtcgacaa gggcgatcag
      841 gacatcatcg cgagccctga gctttattcc ggcggcgagg ggctcgacac ggcttcctta
      901 agtttccgga tcgcggcgat ccgcgaaacc tttgaggaaa gcggcatcct gctggcgcgt
      961 ccacgggaat cgaaagcgct ggtcgatgcc aggcgcgcca gcgcgatcga ggctgcccat
     1021 cgcactgcct tgtgcgaggg caagacgacc ttcctaaaag ttctcaccga tagcggaatg
     1081 ctgctggcac tcgacgaact ggtgccctat gcgcactgga tcacgccgga aggcttgcca
     1141 aaacggttcg acacctggtt ctttcttgcc gccgccccac cggaacaggt cggcgcccac
     1201 gacggcaagg aatccaccga ttcgatctgg gtgtcgccgc gcgaggcgct cgaaggcggc
     1261 aagagcgggc ggttcaagct gccgtttccg accacgcgca acctgatcag gctcggcaag
     1321 cagccaaacg tgaaggccgc gctcgacgac agtattggca aaccgatcgt caccgtgatg
     1381 cctgtggtga caaagcgcga cggcgttcgt cagcttcgga ttccgctcga ggccggctac
     1441 gacggcgagg tgttcgaagt cggcgcggcg tgaatcaccc cgccttcgcc tgcgccctgt
     1501 cttgcccgta aagccttcgc aactcgcgtt tgagcacctt gccggcgccg gagagcggaa
     1561 acggttcggt cctgacgtcg acgctgcgcg gacatttgta gccggcgatc cgctccttgc
     1621 agaacgcgat gagttcggcg gcattgacgt gggcgccggc cttcgggatc acgaaggcgt
     1681 ggaccgtctc gccccattgc tcgctgggga tgccgatcac ggcgcattgc gacaccgccg
     1741 gatgctgtgc gatggcgttt tccacctcca tcgaatagat gttctcgccg cctgaaatga
     1801 tcatgtcttt gacgcgatcg accagataga catagccttc ctcgtccatg tagccgccgt
     1861 cgccggtgtg catccagccg tcgatcacgg ccttggcggt ttcttccggc cgctcccaat
     1921 agcccatcat cacattctgg ccgcgcaccg cgacttcgcc gaccacgccc gatgcaaccg
     1981 gtttgcggtc ggcatcgacg atcctgacct cgcagccgat cgcggcgcgg ccgcaggcgc
     2041 gctgccggtt tttcttcgaa gccgcctcgc cggtgtgctg gtcccacggc agatgcgtcg
     2101 ccagcggaga tagttcggtc atgccgtaga gctggtggaa cgcggtgccg ggcaaccccc
     2161 acatcgcgcg gttcagcagc gcttccgaga tcggggaggc gccgtacatg atccgctgca
     2221 gcgacgacag gtcggcggtc tggaacgcgg gatgatcgac cagcatctgg atcatggtcg
     2281 gcacgatcag ggtggcggtg actttctccc gctcgatggt gtccatcacc agttccggat
     2341 tgaacgtccg cacgatcacg ttggtgccgc cgccgatcag ggaggcaaac atcgcgccgc
     2401 cattggcgag gtgaaacatc ggcgcggcgt tgagatagat cgtgccgtcg ggcaacaggc
     2461 cttcgctcag catgtgcagc gcgttgctga cgatattggc gtggctcagc atcacgccct
     2521 tggagcggcc ggtggtgccg ccggtataga agatgccggc aagctcttcg cgcccggaca
     2581 tcgcgtccgg caccggttgg ctcacagtga tcagtgtttc gtagttctgc gcttcggttg
     2641 gaccggaatc gtcgtcggca tagatcagcg ccagcggaac ggcctttgcc agttcgacgc
     2701 ccatcttggc aaaagcctta tccacgacca gaaccgaggc gcggcagtcg tgcagcgagt
     2761 cctcgatctc ggcgtggctc catcggatgt tggtgggcac aatcacggca ccggcccagg
     2821 cgatgccgag atagagttcg agatagcggt cggcattcaa catcagcacc gcgacgcggt
     2881 cgccgcgatt cacgccgagc tctcgcaggc cgccggcgag ccttgcgacg cgctcgccga
     2941 tctcgcgcca gctgcggcgg cggtcgccgt cgacggtcgc gattccttcc ggattggtct
     3001 gcagcacccg gcgcaggccc tgggtcacgt tcataacgtc tctccctgat attggcgggc
     3061 gcttgatgca cctctggctt cgatcataac gatgcgagtt gaggtcgcaa gcgccaggcg
     3121 ggccttgctg agcgatgcga ctatctgcat cctgtatgcc agcatcctga gcgccggtat
     3181 ccggcgcttt cgcagcgtca tcaagcgatg caaccggtgg atggagttca ggaatgcaat
     3241 ttgccctgag ttgccgcgat gcaccggtca gccttgcgta tcgcgatcgc cacggacggg
     3301 ggcgagatcc tgtgcacgcg gcagccgcgg cgtatctgac cctatcgctc aaaaaaatcc
     3361 ctgcacggaa cgggctggtg tttccatgca gagaagtttg ggactacgcg ttcgagctag
     3421 cggcgttgcg gcgagctgtc agttcaatga gagacagttc ggcaaaacaa cgcctcgcgt
     3481 ggcgctggtg ccctttcttt tggatcgagg ttgctcccta gtgatcctct tttagccggg
     3541 gcttttcatg caagaccgca ctcaacagcg aattgtgaac ctcgatccgc ccgttgtaat
     3601 cgatgaggcc cagcttgcga aacttgttca tgaaatggct gacgcgggat cgagtggtgc
     3661 cgatcatttc cgccagtgtt tcctgactaa tgttcggact gatcggctgc gggctgcctg
     3721 ccttctcgaa attcgcgagc agcagcagca tgcgcgcaag ccgcctctcg ctggaattga
     3781 atagttgatc gatcaggtcc tcctcgatcc ggccgttgcg ggtcaggaga tgcgccataa
     3841 acatttcgga aagtttcggc tcgtcatgaa gggcggccaa catcgcatct ttcgtgatcg
     3901 ccgtaatgac acaatcgacc atggccgttg ccgtcgaaat gtgaaacttg tggccattta
     3961 tgctaccctc accaaggaac tgtccgggtc ccaatatccc gactaccgcc tccttgccct
     4021 gatcggacag gacagtcacc ttgaccttgc ctttttgaat gtagaacacc gtgtccgcga
     4081 catcgccttg cgcgaacaca ttctgattct tttgaaattc caatttggtc tttccagctc
     4141 caacgcccgc gagaaaaacc cgagcgtcaa acacggcttt gcctgcgcgc accatgcaag
     4201 cctcctttgc cgaaacgaac ttcgggataa cgaagcccga ctaggaaggt tgcatatttc
     4261 ggtgcgctca actcgtcgag acagtgctga ctttggactc tctgcgacgg tatcgcgatg
     4321 acttcgaaca tcctcacagc gggctctatg tccggattgg gtcgtgtgtc acgagcgcag
     4381 tctgagtacg acggtgcgga actgtatgag agatgaagga ggcccttcga ggtcggcaat
     4441 caggtgttga tctcaagccg ccgcaagctg ctgtcgtcaa aagcgatggc ggtgaacgtt
     4501 gcggaaaagt cgggacatga tcccggcagg caaggttgag agaggcgagc gcaagcgaac
     4561 cgtcgatgaa gtgtcgaaag cggattagac gatgtcgaaa ctggggggtg acccttcctc
     4621 cgggatcagc ttgggggatg tcctgagact gtccgagcgg catccggcat gtaggctgcg
     4681 cgaagctcaa ccaggctctt gtacggaacg tgagaaccta gtcggcgtca caccgctcgc
     4741 cttcaatggg cggcgtgaag ggacaagcca caagcggcgc caatcgcgag ggcgagagta
     4801 ccgacacgca gggcagggac ggagcagcgg tcggcaagtc cgaaaagtgc caagaagcga
     4861 catgcgcagc gattgcaact aaatttccgc acgggctaaa ccgttagaat tgttggaccg
     4921 aacttctggt gcgtaattct tgatttttgc accggtccaa attttgcggg tctgcgatct
     4981 tttgagaggc aggaatcaag aatgggtttt gagagacggc ggcgcaactg cgagcagtgc
     5041 gcctctttct cacgcctttc gaatgaggat gccccctaaa gatcgaaatt agtgggcagc
     5101 atgaccacgt cgcgaattgt catgccgcgt ggccgcgtca gcatgaacgt caccacgttc
     5161 gccacttcgc tggcctcctg cagacttccc gactccttgg cttccttcag cttctcaggc
     5221 ggccagtccg cgagcagtgc gctgatgacg gggccgggtg agattgagcc cacgcgaatg
     5281 ccgtacttga agacttggcg tcgcacggtc tggacaaagc agttgatcgc ccacttggac
     5341 gacgcataga ccggctccca tggcgtcgga aaatgggccg ccaaggaact cgtgacgatg
     5401 atgtcgcccg tccgacgctc gatcatgtga ggcagcacgt cgtgcacatt cttcatcacg
     5461 acgttgacat tcaagttcgg catccggtcg atagccccag tgtcggcatc gaccaagtcg
     5521 ccgccgacgt acgtacccgc attggcatgc aggatgtcga gttggcctac cttctccagg
     5581 actctcggca gcagggtagc gcagtccttc gggtcgagca ggtcgatgac cagagggatc
     5641 actgcgtcgc cgtgcttgtt acagagcacc ttcagcgctg cctcgtcgcg gtcgaccatc
     5701 accacgcgcg agcctgcggc caacatcgcc tcggtgctag ccaatccgat gcccgacgca
     5761 gccccggtca cgacggcaac cttgccttcc aattctcttg ccataggtct ctctccttgt
     5821 tgggcgccgt gctccttgcg ccagccgatc gcatagcgcc gaacggcggc ttaactcccg
     5881 ccatagccga aaccgcgcag ctactcgaga aggtggcagc tgctcagccc ttactcaaat
     5941 cgacggcagc agatcctatg ggcagcagca gaggccgagg tgatcggtca aagcggctgc
     6001 ctgctgctgg ccagtgtaac gggtatcagg cggcagacta tatcgaagcg gaaagtcccc
     6061 catggggtaa ggactagc
//
LOCUS       DASWGM010000433         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_876879,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000433 DASWGM010000000
VERSION     DASWGM010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876879"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VGA15_12615"
     CDS             <1..1546
                     /locus_tag="VGA15_12615"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX9588567.1"
                     /translation="FPMHPIQETGKRPTAALDRNLAYLGLVEILFGYPLDGVVLTTGC
                     DKTTPACLMAAATVNLPAIVLSGGPMLNGWHNGERSGSGTVVWKARERLAAGEIDYEE
                     FMNIVASSAPSVGHCNTMGTASTMNSLAEALGMSLPGCAAIPAPYRERGQIAYETGTR
                     IVGMVWEDLKPSDILTRQAFENCIVVNSAIGGSTNAPIHINALARHIGVELDIDDWQK
                     YGHEIPLLVNMQPAGFYLGEEYHRAGGVPAVVRELMAHKRIHEDAMTVNGHTMGENCR
                     DAAKPDGDVIWTYDKPLVKDAGFLVLRGNLFDSAIMKTSVISKEFRDRYLINPKDPNA
                     FEGRAIVFEGPEDYHHRIDDPALDIDGHCILFVRGTGPIGYPGGAEVVNMQPPAALIK
                     RGILSLPCIGDGRQSGTSGSPSILNATPEAAADGGLAILKTGDMVRVDLNKGSANILI
                     SDEELKRRRAELKAKGGFPYPANQTPWQELYRSTVGQQATGACLELATRYHDIAGSVG
                     VARDNH"
     gene            1847..2581
                     /locus_tag="VGA15_12620"
     CDS             1847..2581
                     /locus_tag="VGA15_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9588568.1"
                     /translation="MSDRLKGKRAFVTAAAVGIGRACAVAFAREGATVFATDIDEKGL
                     ALLGKDGVAEVARLDVRDSAAVGEMAKRVGKVDILLNAAGFVHHGTVLECSDNDWDFS
                     FDLNVKSMHRTIRAFLPDMLAADRGSIVNISSAAGVFKAAPNRYVYGATKAAVAALTS
                     AVATDFITRGIRCNCICPGTIETPSMLNRAAAAGPGGREMFVSRQPMGRLGTAEEIAS
                     LAVYLASDESSFTTGVAHVIDGGWTL"
     gene            2861..>3318
                     /locus_tag="VGA15_12625"
     CDS             2861..>3318
                     /locus_tag="VGA15_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9588569.1"
                     /translation="MNKIDLNGRCAVVTGGAQGFGRAITERFVSSGAKVAIWDHDQPF
                     AEKTAKEIGSGVTAFKVDVSDPAAVEKATDATLKAFGRIDILVNNAGIAGINKTVWET
                     DLEEWRKVLRINLDGPFICCKAIVPSMVKQNYGRIVNIASIAGKEGNPNAA"
BASE COUNT          647 a         1078 c         1037 g          556 t
ORIGIN      
        1 atttccgatg cacccgatcc aggagaccgg caagcgcccg accgcggcgc tcgaccgcaa
       61 cctcgcctat ctcgggctgg tggaaatcct gttcggctac ccgctggacg gcgtggtcct
      121 gaccacgggc tgcgacaaga ccacccccgc ctgcctgatg gcggcggcga cggtcaattt
      181 gccggcgatc gtgctgtcgg gcgggccgat gctcaacggc tggcacaatg gcgagcgctc
      241 cggttccggg accgtggtct ggaaagcgcg cgagcggctc gccgccggcg agatcgacta
      301 cgaggaattc atgaatatcg tggcctcctc ggcgccgtcg gtcggccatt gcaacaccat
      361 gggcaccgcc tccacgatga attcgctggc cgaagcactc ggcatgtcgc tgccgggctg
      421 cgcggcgatc cccgccccct atcgcgagcg cggccagatc gcctatgaga ccggcacgcg
      481 catcgtcggg atggtctggg aggatctcaa accgtccgac atcctgacgc ggcaggcatt
      541 cgaaaactgc atcgtcgtca attcggcgat cggcggttcg accaacgccc cgatccacat
      601 caatgcgctg gcccgccata tcggcgtgga actcgacatc gacgactggc agaaatacgg
      661 ccacgaaatt ccgctgctgg tgaacatgca gccggcgggc ttctacctcg gcgaggaata
      721 tcatcgcgcc ggcggcgtgc cggcggtggt gcgcgaattg atggcgcaca agcgcatcca
      781 tgaagacgcc atgaccgtca acggccacac catgggcgaa aactgccgtg acgccgcaaa
      841 acccgatggc gacgtgatct ggacctacga caagccgctg gtgaaggacg ccggtttcct
      901 ggtgctgcgc ggcaatctgt tcgactccgc gatcatgaag accagcgtga tttcaaagga
      961 attccgcgac cgctatctga tcaatccaaa ggacccgaac gcgttcgagg gccgcgccat
     1021 cgtgttcgag ggcccggagg attatcacca ccggatcgac gatcccgcgc tcgatatcga
     1081 cgggcactgc atcctgttcg tacgcggtac cggtccgatc ggctatcccg gcggcgccga
     1141 ggtcgtcaac atgcagccgc ccgcggcgct gatcaaacgc ggcatcctgt ccctgccctg
     1201 tatcggcgat ggccgtcagt ccggcacttc gggctcgccg tcgatcctga acgccacgcc
     1261 cgaagccgct gccgacggcg ggctggcgat cctcaagacc ggcgacatgg tccgcgtcga
     1321 cctcaacaag ggcagcgcca acatcctgat ttccgatgag gagctgaagc ggcggcgcgc
     1381 cgaactgaag gcaaaaggcg gcttccccta tccggccaac cagacgccct ggcaggagct
     1441 ctatcgctcg accgtcggcc agcaggccac cggcgcttgc ctggagctcg cgacgcgcta
     1501 ccacgacatc gccggaagcg tcggcgtggc acgggataat cactaggctg cttcgatatg
     1561 ttcagcattc agccgcgacg catgcacggt ccacctctcc catcgggaga ggtcggatcg
     1621 tatcgacgac gtcgagacga tccgggtgag gggttaggtc ccatcgatag accgtaaccc
     1681 ctcaccccga ccctctccca tcgaagtcgg atatatccga cttcgacaga ttaagatgct
     1741 caactcgggt aaacccgagt tgagtgggag agggagcgcg ctgccgtcgt ggccgcatag
     1801 aaaaccccag ctaatcagaa cttcaacgaa atttcgggag aacgaaatgt cagaccgcct
     1861 taagggcaaa cgcgcgttcg tcacggcggc tgcggtggga atcggccgcg cctgcgccgt
     1921 cgcctttgcg cgcgaagggg cgacggtatt tgccaccgac atcgacgaga agggtcttgc
     1981 gctgctcggc aaggatggcg ttgcggaagt ggcgcggctc gacgtgcgtg actctgccgc
     2041 cgtcggggaa atggcaaaac gtgtcggcaa ggtcgacatc ctgctcaatg cggccggctt
     2101 cgtgcatcac ggtaccgtgc tcgaatgctc ggacaatgac tgggattttt cgttcgacct
     2161 caacgtcaag tcgatgcacc ggaccatcag ggcctttctg cccgacatgc tggcggccga
     2221 ccgcggctcg atcgtgaaca tctcgtcggc cgccggcgtg ttcaaggccg cgcccaaccg
     2281 ctacgtctat ggcgcgacca aagccgccgt cgcggcgctg accagcgcgg tcgccaccga
     2341 cttcatcacc aggggcatcc gatgtaattg catctgcccc ggcaccatcg agacgccatc
     2401 catgctgaac cgcgccgctg ccgcgggtcc tggagggcgc gagatgttcg tgagccgcca
     2461 gccaatggga cggctcggca cggccgagga aatcgcctcg ctcgcggtct atctcgccag
     2521 cgacgagagc tcattcacga ccggcgtcgc gcatgtcatc gacggtggct ggacgctgtg
     2581 attgaaggcg cgcttcttca cctcgccccg ctctttgcgg ggagaggtcg gaaactgcgc
     2641 caacgggtcg gcgcgaagcg ccgcccgatg ataaactccg cggagtttcc gggtgagggg
     2701 caaggcacgg tgctcaattc atccgcggca ctcgcgggtc ggagagcccc tcaccccaac
     2761 cctctccccg cgaagagcgg ggcgagggag caggcttccg ccggcgggac gccgatagtc
     2821 acaccggtgc ggtagaattt tagcgagagg acttctcacc atgaacaaga tcgatctgaa
     2881 cggccgctgt gccgtcgtca ccggcggcgc gcaggggttt gggcgcgcca tcaccgagcg
     2941 ttttgtcagt tccggcgcaa aagtcgcgat ctgggatcac gatcagccgt tcgccgagaa
     3001 aaccgccaag gaaatcggct caggcgttac ggcgttcaag gtcgacgtgt cagatcccgc
     3061 cgcggttgaa aaggcgacag atgccacact caaggcgttc ggcagaatcg acattctcgt
     3121 caacaatgcc ggcatcgccg ggatcaacaa gacggtatgg gaaaccgacc tcgaggaatg
     3181 gcgcaaggtt ttgcgcatca atctcgacgg gcccttcatc tgctgcaagg cgatcgtgcc
     3241 ttcgatggtc aagcagaatt acggccgcat cgtcaacatc gcctcgatcg ccggcaagga
     3301 aggcaacccc aacgcggc
//
LOCUS       DASWGM010000434         5208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_724838,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000434 DASWGM010000000
VERSION     DASWGM010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724838"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..437
                     /locus_tag="VGA15_12630"
     CDS             204..437
                     /locus_tag="VGA15_12630"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9588570.1"
                     /translation="MNLRAIANPVWMKHRRETAEHPFGTMKWLMAHPRFLLRGLRKAK
                     AELALGVLCYNLKRVVNILGVAALLQALEPSPA"
     gene            complement(550..1032)
                     /locus_tag="VGA15_12635"
     CDS             complement(550..1032)
                     /locus_tag="VGA15_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX9588571.1"
                     /translation="MQDLVAIAEKIAAHLIERRQTIAVAESSTGGLISAALLAVPGAS
                     AYFLGGAVVYTRDARRILMDIPDDAMKGIRSASEPYAKLLASQMRTRFATDWGLSETG
                     ATGPTGNRYGDAAGHSCIGVAGLGASAMTLETGSADRVANMQVFAATALNFLLQTLSR
                     "
     gene            complement(1032..1982)
                     /locus_tag="VGA15_12640"
     CDS             complement(1032..1982)
                     /locus_tag="VGA15_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9588572.1"
                     /translation="MTTVRMAVVATMMAWSAPAFAQTPPTTPPPPDFSKVEIRTTDLG
                     DNCYMLEGQGGNITVAAANDEIIMVDGEFAPLHDKIRAAISTVSNLPVKYLVNTHFHG
                     DHTGGNEPFAKDGATVVAEINVKNRLAAGTTNGLTGVQTPPAPQGALPTKTYTGAYKI
                     RLRGRVADLRHIANAHTDGDTYVWFKTANVLATGDTFTNGRYPNIDFANGGNIKGMIA
                     AADTYLKLTNAKTRIVPGHGPLADKAALMDYRTMLVTARDRMAKLVKAGKSEDDVVAA
                     KPFGDFDAKWAPTELAAKNFVRVVYHSLADKPKDKTKKGA"
     gene            2146..3300
                     /locus_tag="VGA15_12645"
     CDS             2146..3300
                     /locus_tag="VGA15_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9588573.1"
                     /translation="MTSPIAGGVDCDLHPAVPHLTSLLPYLNDYWRDQVTTRGMTDLV
                     SQSYPPHSPISSRPDWRPARGKPGSNLEDMKAQALDRFGVSYGICNPLYGVQMVFSED
                     MADAFCRALNDWLVKEWLDKDARLRGSIVIPVQSIEKSVAEIERCAADKRFVQVLMLV
                     MGDMPLGKRAYWPIYAAAERLGLAVGIHAGSNYHNPPTSVGWGSYHIEDYVAQAQAFQ
                     TQLTSLIVEGVFAKYPKLKMVMLESGFTWLPSYLWRLHKFWRGVRMETPWVDRAPLEI
                     VRSNIRFSLQPIDAPPDPATLNRLFDHMQSDELLLFSTDYPHWQFDGDAVLPEGMSSD
                     LVRKIMIDNPHATYTRLKEPAHGHDPEKHALGPRPDGWNPVFGKDHAQTK"
     gene            3358..4467
                     /locus_tag="VGA15_12650"
     CDS             3358..4467
                     /locus_tag="VGA15_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9588574.1"
                     /translation="MNVQFRSGAEPASSVGVKTAIADCDIHPARATATELYPYLAKRW
                     HEHLETYGKHPYQGMMEGPPYPKAQPNASRRDAWPPEGGPQGSSLSFMQQQHLDPNNV
                     ALGVLCPLNTGQGIRNQDLSAALATAINDWQIDKWTSQDSRLKGSVVVANEDGLAAAA
                     EIRKRAGDANFVQVLLLSRNVEPLGQRRYWPIYEAAEEAGLPIGVHAFGFGGNPITAS
                     GWPSYYIEEMVGHSQCQQTALASIVLEGVFERHPKLKMVMIEAGFGWAPSLSWRLDKS
                     WQRLRSEVPHVKRPPSETIREHIYWTTQPMEDPERRDHLFEVIDWVGWDKLLFATDYP
                     HWDYDDPSRVLPAGVSAANREAFYLGNARKVYGLA"
     gene            4525..4938
                     /locus_tag="VGA15_12655"
     CDS             4525..4938
                     /locus_tag="VGA15_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX9588575.1"
                     /translation="MTRHVIAPVDELPPGTRKFLTINARPIAVFNIKGEFFGLLNRCP
                     HQGAALCEGPLIGLAQSSDPGEIEYTRPGEIIRCPWHGWEFDIRTGQSYCDPKRFRAK
                     TYPANVEPGASVVKGPYVAETIAVSVESDYVVVDL"
     gene            complement(5053..>5208)
                     /gene="tcuB"
                     /locus_tag="VGA15_12660"
     CDS             complement(5053..>5208)
                     /gene="tcuB"
                     /locus_tag="VGA15_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846806.1"
                     /note="with CitA (TcuA) catalyzes the oxidation of
                     tricarballylate to cis-aconitate; originally thought to be
                     involved in citrate utilization; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization protein TcuB"
                     /protein_id="HEX9588576.1"
                     /translation="DTAAMGPLLALHLGLVFSLFITMPYGKFVHGIYRYVALVRYARE
                     RRMLGHV"
BASE COUNT          984 a         1573 c         1665 g          986 t
ORIGIN      
        1 tcggcagctc tatccgatgg cggcgcaggc caaggatgcc gtaggcgccg atcaggtgac
       61 ggcggtggcc gacaccggct attcgaacgg cgagcatggc gcgctctgcg agcaggacgg
      121 catcaccgct gttgtgcccc gtgccgagac tgtcaatccg aacgggcccc aatacttcag
      181 ccgtgaccgg ttcagctacg atcgtgaatc tgcgggcgat cgccaacccg gtatggatga
      241 agcatcgccg ggaaacggcc gaacatccgt ttggcacgat gaagtggctg atggcccatc
      301 cccggttctt gctgcgcgga ttgagaaaag cgaaagcgga actggcgttg ggcgtgctgt
      361 gctacaatct caaacgggtg gtcaacatcc ttggggtggc ggcgctgctg caagcgcttg
      421 agccttcccc cgcctgagaa ggatttctct gccgcaaaca tcgtcgaacc cctcagtcgc
      481 aaaacccccg acttttgata cagcctggaa ggcgggacga cgatgagggt gggcggcgtt
      541 tgcgaatcct caccgcgaca aagtctgcag caaaaaattc agtgccgtcg ccgcaaacac
      601 ctgcatgttg gcgacgcggt cggcgctgcc ggtttccagc gtcatcgcag acgcccccaa
      661 tcccgccacg ccgatgcagc tgtggccggc ggcgtcgccg tagcggttgc cggtcgggcc
      721 ggtggcgccg gtttcggaca ggccccaatc ggtggcaaag cgtgtgcgca tctggctcgc
      781 gagcaatttg gcgtaaggct ccgaggccga gcggattccc ttcatggcgt cgtcgggaat
      841 gtccatcagg atccgccgcg catcgcgggt atagaccacc gcgccgccga ggaaataagc
      901 cgacgcgccg ggcaccgcga gcagtgccgc cgagatcagg ccaccggtcg aggattccgc
      961 caccgcgatg gtctggcgcc gctcaatcag gtgagccgct attttttcgg caatcgcaac
     1021 gagatcctgc atcacgcacc tttcttggtt ttgtccttag gcttgtcggc gagcgaatgg
     1081 taaaccacgc gaacaaaatt cttggcggcc aactcggtcg gcgcccattt ggcatcgaaa
     1141 tccccaaatg gttttgccgc gaccacgtcg tcctcgctct tgcccgcctt gaccagcttt
     1201 gccatgcggt cgcgcgcggt caccagcatg gtgcggtagt ccatcagcgc ggccttgtcg
     1261 gcgagcggcc cgtggcccgg cacgatccgg gttttcgcat tcgtcagctt cagataggta
     1321 tcggcggcgg cgatcatgcc cttgatgttg ccgccattgg cgaaatcgat attgggatag
     1381 cggccgttgg tgaaggtgtc gccggtcgcg agcacattgg cggtcttgaa ccagacgtaa
     1441 gtgtcgccgt cggtatgcgc attggcgata tgcctgaggt cggcgacgcg gccccgcagc
     1501 ctgatcttgt aggcgccggt gtaggtcttt gttggcagcg cgccctgcgg cgccggcggc
     1561 gtttgaacgc cggtcaggcc gttggtggtg ccggcggcga ggcgattctt gacgttgatc
     1621 tcggccacaa cggtggcgcc gtctttcgca aaaggctcgt tgccgccggt gtggtcgcca
     1681 tgaaaatgcg tgttgacgag atatttgacc ggcaggttgg agaccgtcga gatggcggcc
     1741 ctgatcttgt catgcagcgg cgcgaactcg ccgtccacca tgatgatctc gtcgttcgcg
     1801 gccgccaccg tgatgtttcc gccctggcct tcgagcatgt agcagttgtc gccgagatcg
     1861 gtggtcctga tctcgacctt ggaaaaatcc ggcggcggcg gggtggtcgg cggcgtctgc
     1921 gcgaaggccg gcgcggacca cgccatcatg gtggctacga cggccattct cactgttgtc
     1981 attcgggtcc cccagcgacg attttgggat gtcctagcac aggattgcat gggtgccgac
     2041 tctgaggtgc atcggcccgc cgatggacgg cgctgcctgc gcgccatatg ctgtctcgaa
     2101 caagcggtcc gcgctgaagc cgacgggcaa gaggaaacga aaatcatgac gtcaccgatc
     2161 gcgggcggcg tggattgcga tctgcatccc gccgttccgc atttgaccag cctgttgccc
     2221 tatctcaacg attactggcg cgatcaggtc accacgcggg gcatgaccga tctggtgtcg
     2281 caatcctatc cgccccattc gccgatcagc tcgcggccgg actggcggcc ggcgcggggc
     2341 aagccgggct ccaacctaga agacatgaag gcgcaggcgc tcgatcggtt tggcgtcagc
     2401 tacggcatct gcaatccgct atacggcgtg cagatggtgt tttcggagga tatggcggat
     2461 gcgttctgcc gcgcgctcaa cgattggctg gtgaaggaat ggctcgacaa ggacgcgcga
     2521 ttgcgcggct cgatcgtgat acccgtgcag agcatcgaga aatcggtcgc ggagatcgag
     2581 cgctgcgccg cggacaagcg cttcgttcag gtgctgatgc ttgtgatggg cgacatgccc
     2641 ttgggcaagc gcgcctactg gccgatctac gccgctgccg agcggctggg cctggccgtc
     2701 ggcattcacg ccggcagcaa ttatcataac ccgccgacgt cggtcggctg gggttcctat
     2761 cacatcgagg attacgttgc gcaagcgcag gcgttccaga cccaactgac cagcctgatc
     2821 gtcgagggcg tattcgcgaa atatccgaag ctgaaaatgg tgatgctgga gtcaggcttc
     2881 acctggctgc cgtcctatct gtggcggctg cacaagttct ggcgcggtgt gcgcatggaa
     2941 acgccgtggg tggaccgcgc gcccttggaa attgtgcgca gcaacatccg gttttcattg
     3001 cagccgatcg acgcgccgcc ggatccggca accctgaacc gcctgtttga ccatatgcag
     3061 tcggatgaat tgctcctatt ctcgaccgac tatccgcact ggcaattcga cggcgacgcg
     3121 gtgctgccgg aaggaatgtc ctcggatctc gtgcgcaaga tcatgatcga caacccgcac
     3181 gcgacttaca cgcgcctgaa agagccggcg cacgggcatg atcccgaaaa gcatgccctc
     3241 gggccacgac ccgatgggtg gaatccggtt ttcggaaaag atcatgccca aacaaaataa
     3301 tttcgacgag tctaattcag cgaagctgga ttagactctg gagaaggaga cgacgccatg
     3361 aacgtgcagt ttcgcagcgg tgccgaaccc gcctcatccg ttggcgtcaa aacggcgatt
     3421 gccgattgcg atatccatcc ggcgcgcgcg acggcgacgg agctttatcc gtacctcgca
     3481 aaacgctggc acgagcatct cgaaacctac ggcaagcatc cctatcaagg catgatggaa
     3541 ggcccgccct acccgaaggc gcagccgaac gcgtcgcgcc gcgatgcctg gccgccggaa
     3601 ggcgggccgc agggctcctc gctgtcgttc atgcaacaac agcatctcga tccgaacaac
     3661 gtcgcgcttg gtgtgctgtg tccgctcaac accgggcagg gcatccgcaa ccaggacctg
     3721 agtgccgcgc tggcgaccgc catcaacgac tggcagatcg acaaatggac cagccaggat
     3781 tcgcgtttga aaggctccgt cgtggtcgcc aacgaagatg gcctggccgc ggccgccgaa
     3841 atccgcaagc gcgcaggtga tgcgaatttc gtccaggtgc tgctgctcag ccgcaacgtc
     3901 gagccgctgg gtcagcgccg ctactggccg atctacgagg cggcggagga ggcggggctt
     3961 ccgatcggcg tgcatgcgtt tgggtttggc ggcaatccga tcacggcatc gggctggccg
     4021 agctattaca tcgaggagat ggtcggtcat tcgcaatgcc agcagaccgc gctcgccagc
     4081 atcgtgctgg agggcgtgtt cgagcggcac ccgaagctga agatggtcat gatcgaggcc
     4141 gggttcggct gggcgccgtc gctgtcgtgg cggctcgaca agagttggca gcgtttgcgc
     4201 agcgaggtgc cgcacgtaaa acggccgccg tcggaaacga tccgcgagca catctactgg
     4261 accacgcagc cgatggaaga ccccgagcgg cgcgatcatc tgttcgaggt gatcgactgg
     4321 gtcggctggg acaagttgct gttcgcgacc gactatccgc actgggacta tgacgatccg
     4381 tcgcgggtgc tgccggccgg tgtcagcgcc gccaaccgcg aggcgtttta tctcggcaat
     4441 gcgcggaagg tttatgggct cgcatagcat gatccggaaa agtgggaacc ggttttccga
     4501 aaagatcatg cgtcagaaaa taacatgact cgtcatgtca tcgccccggt ggacgagctg
     4561 ccgccgggca cgcgaaaatt tctcaccatc aatgcgcgcc cgatcgcggt gttcaatatc
     4621 aagggcgagt tcttcggcct gttgaaccgc tgcccgcatc aaggtgctgc gttgtgcgaa
     4681 gggccgctga tcgggctcgc gcaatcgtcc gatcccggtg agatcgaata taccaggcca
     4741 ggcgaaatca tccgctgccc ctggcacggc tgggaattcg atatccgcac cggccagtcc
     4801 tactgcgacc ccaaacggtt tcgcgcaaaa acctatcccg ccaatgtcga gccgggtgca
     4861 agcgtggtga aggggcccta cgtggcggag acgatcgcgg tgtcggtcga gagcgattat
     4921 gtggtggtgg atttgtgatg tcattccggg gcgcgcgcca gccacttttc ccatcactgc
     4981 gaggagcgaa gcgacgaagc aatccactct ttgctagtca tggattgctt cgcttcgctc
     5041 gcaatgacgg cgctaaacat gccccaacat ccgccgctcc cgcgcatagc gcaccagcgc
     5101 gacatagcga tagatgccgt gcacgaactt gccgtagggc atggtgatga acagggaaaa
     5161 cactaagcct agatgcagcg ccagcagcgg ccccatcgca gccgtatc
//
LOCUS       DASWGM010000435         3846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1553499,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000435 DASWGM010000000
VERSION     DASWGM010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553499"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..2708
                     /locus_tag="VGA15_12665"
     CDS             213..2708
                     /locus_tag="VGA15_12665"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9588577.1"
                     /translation="MTTASEHLFKKAIEAHRNGAVENAKILYREVLTADPKNAAAYGN
                     LAIIAAQQGDLAGAERLMRQEIRLRPNTPAGYHNLGSVLQQQGRLADAIAAHRHAIKL
                     KPDYAEAFLALGNGLRQQGHLEEAMGSYRSALAARRDYPEAHNNIGVLLQMQGRLEQA
                     AAAYHEAVRLRPGYAEAQFNLGTVLHQRHELDAAEAAYRRVISLNPGIAAGHNNLGTV
                     LKDQGLLDGALAAFDQAIALQADYAEAFYNRATVLRQQARLEEALAAYGRAIALRKNY
                     ADAINNAGIVLQELGRAREAVDLYRQLLEQMPAHADACNNLGTALLAEGRADEARAAF
                     EQALAHKPDFPEAFCNLGNARRELGDLAGAIAAYRDALRLRPDDAGAFSQLIYHRAQA
                     CAWDGYEADQEKLVDMARRGIRVPPFYLLSTPASASDQLSCARHWATSISPPRKALFH
                     HQPRAERERIRLGYLSGDLHQHATAQLMAELFECHDRDRFEVFAYSYGPDDDSPMRAR
                     LAAAFDRFVDIRARSHRKAADLIHADQVDILVDLKGYTHHARPAISAYRPAPVQVSYL
                     GYPATMGADFIDYIIVDPFVVPSSQQPFFSERLVHLPRSYQANDRRREVADARPSRRD
                     FGLPAEGLVLCSFNNNYKISPAFFDIWMRLLRAVPGSVLWLLEANPLVRGNLRSEAEK
                     RGVDSGRLIFAPLVPPAEHLGRHRHADLFLDTLPCNAHTTASDALWAGLPVVTCCGDT
                     FAGRVASSLLMAIGMPELVTKSLEEYEQAALALARSPQRLIALRQKLQNNRDASALFD
                     LPKLTGNIEAAYARMWQTWLAGQTPAAFSIEGA"
     gene            2726..3040
                     /locus_tag="VGA15_12670"
     CDS             2726..3040
                     /locus_tag="VGA15_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588578.1"
                     /translation="MLMRTIVTLAAILALGSSHGWANAQKAPRHPTRRHRHGYIVRYQ
                     EQPVLPAPYGGGRLVLHPASNIACMTRDRATRALPCDQPVWVYGSPCEVDLGLGRYRS
                     CD"
     gene            3071..>3846
                     /locus_tag="VGA15_12675"
     CDS             3071..>3846
                     /locus_tag="VGA15_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9588579.1"
                     /translation="MNAPGRSEQTLAAAMAHHRAGRLTDAERLYRIVCDIDPKNARAF
                     HLLGVVAHQLRRPDAASYLGRAVTLDPHFAEAHNDRGVILAAGGLLADAISCFERAVT
                     LNPGYGEARTNLGRGLRSLGRLDEAVAQFELVLKGTPESPVAHFNLASAFELAGQHPN
                     AEKHYRRAISLRPDFVDAYIHLASLLQNTDRLPEALAHAERAVALRPDSAGVRNNLGN
                     ILRALGRRDEAIAQYQKALGIDPGSFMAHYNCGVALRGET"
BASE COUNT          736 a         1207 c         1179 g          724 t
ORIGIN      
        1 cccggttacg aaaattacgc gacgatattc aagggcccgc gatcgatgga agaggtgcgg
       61 ctcgccggcc cgtttgtcta cgtcaaatta tacggcgcgc agagttttga gatccaatat
      121 accaactacc ggcaaccctg agaatccgga cgggctctcg ccgggggagc agcgcgacga
      181 agcaatccag tgaccgccat ggcgcgcccc tgatgacgac ggcatcggaa catctcttca
      241 agaaagcgat cgaagcgcat cgcaacggcg cggtcgaaaa tgcaaaaatt ctctatcggg
      301 aagtgttgac ggctgatccc aagaacgctg ccgcctacgg caaccttgcc atcatcgcgg
      361 ctcagcaggg cgatctcgcg ggcgccgagc ggctgatgcg gcaagagata aggctgaggc
      421 cgaatactcc ggcgggctat cacaatctcg gatcggtgtt gcagcagcag ggcaggctgg
      481 ccgacgccat cgccgcgcac cggcacgcca taaaactcaa accggattat gccgaagctt
      541 ttttggcgct cggcaatggc ctcaggcagc aaggccatct cgaagaggcc atggggtcct
      601 atcgctccgc ccttgcggcg aggcgcgatt atccggaagc ccacaataac atcggcgtgc
      661 ttctccagat gcaaggcagg ctcgagcagg cggcagcggc ctatcacgag gcggtcaggc
      721 tgaggccggg atacgcggag gcccaattca atctcggcac cgtcctgcac cagcggcatg
      781 aactggatgc cgccgaagct gcgtatcgac gggtcatttc gctcaatccc ggcattgccg
      841 cgggccacaa caatctgggg accgtgttga aggaccaggg cctcctcgac ggggcgctgg
      901 cggcttttga ccaagccatc gcgctgcagg cggactacgc cgaggcgttc tacaatcgcg
      961 ccaccgtgct gcggcagcag gcgcgattgg aagaagcgct ggcggcttac ggccgcgcca
     1021 ttgcccttcg caagaattac gcggatgcga tcaacaacgc cggtattgtg cttcaggaac
     1081 tcgggcgcgc gcgcgaggcg gtcgatctgt atcgccagct gcttgaacag atgccggctc
     1141 acgccgatgc ctgcaacaat ctcggaacgg cgttactggc ggaggggcgc gctgacgagg
     1201 cacgagcggc gttcgaacag gcattggccc acaaaccgga ttttcccgag gcattttgca
     1261 atctgggaaa tgcccggcga gaactcggcg atctcgcagg cgcgatcgcc gcctatcgcg
     1321 atgcattgcg tctgcggccc gacgacgcgg gcgcgttcag ccaattgatt tatcatcgcg
     1381 cccaggcgtg cgcgtgggac ggttatgaag ccgatcagga aaaactggtc gacatggcac
     1441 ggcgcggaat tcgtgttccg ccgttttacc tgctctcgac gccggcgtcg gcatcggatc
     1501 aattgagctg tgcccggcat tgggccacat cgatcagtcc gccgcgaaaa gccttgtttc
     1561 accatcagcc tcgcgccgag cgagagcgca ttcgtcttgg ctatctgtcc ggagatttac
     1621 atcaacacgc caccgcgcag ctgatggccg aattgttcga atgccacgat cgtgaccgct
     1681 tcgaggtttt cgcctattcc tacggccccg atgatgatag cccgatgcgg gcgcggctgg
     1741 ctgccgcgtt cgatcgtttt gtcgacattc gcgcgcgctc acaccgcaag gccgcggacc
     1801 ttatccatgc cgaccaggtc gatatcctgg ttgatctgaa gggctatacc catcacgccc
     1861 ggccggcgat atcggcctat cgcccggcgc cggtgcaggt gagttatctc ggatatcccg
     1921 ccaccatggg cgcggacttc atcgactaca tcatcgtcga ccccttcgtc gtcccaagta
     1981 gccagcagcc gttcttttcg gaaaggctgg ttcatcttcc ccgcagctat caggcgaacg
     2041 acagaaggcg tgaggtggcg gatgcccgcc cgtcgcgacg ggattttggg ctgccggccg
     2101 aggggctggt gctctgcagt ttcaacaaca attacaagat ctcgccggca ttcttcgata
     2161 tctggatgcg tctgcttcgc gcggtccccg gcagcgtatt gtggttgctc gaggcaaatc
     2221 cgctggtccg gggcaatctg cgttctgagg cggaaaaacg cggcgtcgat tccggccggc
     2281 tgatctttgc gccgctcgtt ccgccagccg aacatctcgg acgccaccgg catgccgatc
     2341 tgtttctcga cacgctaccg tgcaacgcgc acacaacggc cagcgatgcg ctgtgggccg
     2401 gactgccggt agtgacctgc tgcggagaca ctttcgcggg ccgtgtcgcc agcagccttc
     2461 tgatggcgat cggcatgccg gaactggtga cgaaatcgct tgaagaatat gaacaggcgg
     2521 cgctggccct ggcgcggagc ccgcaacgcc tgatcgcgct gcggcagaag ctgcagaaca
     2581 atcgcgacgc cagtgcatta tttgatctgc caaaattgac cggaaatatc gaggcggcct
     2641 atgcgcggat gtggcagact tggcttgccg gccagacgcc ggccgcattc tcgatcgaag
     2701 gcgcttagaa taaaggacag ggatcatgct gatgcgaacc attgtcacgc tcgccgccat
     2761 tctcgccctc ggcagcagcc atggatgggc caatgcccaa aaggcgccaa gacatccgac
     2821 aaggcgtcat cgccacggct acatagtccg ctatcaggaa caacccgtcc tgccggctcc
     2881 ctatggcggc ggccggctgg ttcttcatcc cgcttcgaac atcgcatgta tgacgcggga
     2941 ccgggcgacg cgcgcgctgc cctgcgatca gccggtctgg gtctatggca gcccctgcga
     3001 ggtcgacctc ggcctcgggc gctatcggtc ttgcgactga cgtgagatta ttccggttcg
     3061 agatatcgcc atgaacgcgc cgggccgctc cgaacagacg cttgccgcgg ccatggctca
     3121 tcatcgcgcc ggccgcctca ccgacgctga acgcctctac cggatcgtct gcgatatcga
     3181 tcccaaaaac gcccgggcgt ttcatctctt gggcgtggtc gcccatcaat tgagacgccc
     3241 cgatgcagcg tcgtatctcg gccgggccgt gacgctcgat ccgcattttg ccgaggcgca
     3301 caacgacaga ggcgtgattc tcgcagcagg cggattgctg gcggatgcca tatcctgctt
     3361 cgagcgggcc gtgacgctta accctggata tggcgaggct cgcaccaatc tcgggcgggg
     3421 attacgatcg cttggacgcc ttgacgaagc ggtggcgcag tttgaactgg tcctgaaagg
     3481 cacaccggaa tccccggtcg cgcatttcaa tctggcctcg gcgttcgaac tggccggcca
     3541 acatccgaac gccgagaagc attatcggcg tgcgatttcg ctgcgccccg attttgtcga
     3601 tgcttacatc catctggctt ccctgcttca gaatacggat cgtctgcctg aagcgctcgc
     3661 gcatgcggag cgcgccgtcg cgctgcggcc ggacagtgcc ggcgtgcgca acaatcttgg
     3721 caatattctc cgcgcacttg gacggcgcga cgaggcgatc gcgcaatacc agaaggcgct
     3781 cgggatcgat ccgggctcct tcatggctca ttacaattgc ggcgtagcgc tccgcggcga
     3841 gaccag
//
LOCUS       DASWGM010000436         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1840646,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000436 DASWGM010000000
VERSION     DASWGM010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840646"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..939
                     /locus_tag="VGA15_12680"
     CDS             34..939
                     /locus_tag="VGA15_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9588580.1"
                     /translation="MTATKRPAASSEHDITERIAGALEVIASHLAAASPTSGASDSFG
                     SADAFVWHPNGRLAPVPRVSRVDLDLLKGIDRMRDILIENTERFANGLPANNALLWGA
                     RGMGKSSLVKATHATINVNRKPPERLKLIEIHREDIESLPALMALLRTSDFHFIVFCD
                     DLSFDGNDASYKSLKAVLEGGIEGRPDNVILYATSNRRHLLPRDMMENERSTAINPGE
                     AVEEKVSLSDRFGLWLGFHRCSQDEYLAMVKGYCGHFGIKLAEDELEREALEWSTTRG
                     SRSGRVAWQFTQELAGRLGVRLSGK"
     gene            966..2066
                     /locus_tag="VGA15_12685"
     CDS             966..2066
                     /locus_tag="VGA15_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588581.1"
                     /translation="MKRRAILVGITALLAPRSLIAQTPSRDGPRRLGVLMGNLANDPV
                     GQTYTASLMRGLRALDWHEGGNLRIDWRWAGGDPALFDRYATELVELGSDVLLAQSSP
                     SVVALRRKTSTIPIVFTMVTDPIGQGFVENLARPGGNITGFSDFNALMAGKWLEMLTQ
                     VAPPVARVAVLYNPETAPYAGLMMRAIDDAAPSFAITAQSAPCHDAAEIQSMMTELAR
                     EERGGLLVLTDIFTIVHRDTILASAAQHRLPTVYFARSFTTAGGLMSYGIDYAELFLR
                     SAAYIDRILKGASPRDLPVQQPTKFELVINLKTAKARGISLSAKLPVIPRHRVPPSAS
                     PMTGSSGNPVRRGFTALSQALLEYWIARSSPT"
BASE COUNT          434 a          704 c          647 g          412 t
ORIGIN      
        1 aaaacaagac ttggtgtctc gcgaaaatcg gcgataaccg cgacaaaacg tccggcggca
       61 tcttccgaac atgacataac ggaaaggatc gccggcgcgc tcgaggtgat cgcctcgcac
      121 ctcgccgccg catcgccgac gtcaggcgcc agcgactcat tcggcagcgc cgacgccttc
      181 gtctggcacc cgaacggacg gcttgcgccg gtgccgcgcg tcagccgcgt cgatctcgat
      241 ctgctcaagg gtatcgaccg gatgcgcgac atcctgatcg agaataccga acgcttcgcc
      301 aacggacttc ccgccaacaa cgcgttgcta tggggtgcgc gcggcatggg caaatcgtcg
      361 ctggtcaagg cgacgcatgc caccatcaat gtgaaccgca agccccccga gcgtttgaaa
      421 ctgatcgaga tccatcgcga ggatatcgaa agcctgccgg cgctgatggc gctgctgcgc
      481 acctcggatt ttcatttcat cgtgttctgc gacgacctat cgttcgacgg caacgatgcc
      541 tcgtacaaat cgctgaaggc ggtactggaa ggcggcatcg agggtcggcc cgacaatgtc
      601 atcctgtacg ccacctcgaa ccgccggcat ctgttgccgc gcgacatgat ggagaacgaa
      661 cgctccaccg cgatcaatcc cggcgaggcg gtcgaggaaa aagtctcgct ctcggaccgg
      721 ttcggattgt ggctcggctt tcaccgctgc agccaggacg aataccttgc catggtgaag
      781 ggttattgcg gccatttcgg catcaagctc gccgaagacg agcttgagcg cgaagcgctg
      841 gaatggtcga ccacgcgcgg ctcgcgttcg ggtcgcgtcg cctggcaatt cacgcaggaa
      901 ttggcgggaa ggctgggggt aaggctgagc gggaagtaga caccgcgcca gggaggctgt
      961 ctgtcatgaa acgccgcgcg atactcgtgg gaattaccgc tctgcttgcg ccgcgctctc
     1021 tgattgcgca gacgccgtcg cgcgatggac cgcgccgcct cggcgtgctg atgggaaatc
     1081 tcgccaacga cccggtggga caaacataca ctgcttcact gatgcggggc cttcgcgcgc
     1141 tcgactggca cgagggagga aatttgcgaa ttgactggcg ctgggccggc ggcgaccccg
     1201 cacttttcga tcgttatgcg acggaactgg tggagctcgg ctccgacgtg cttcttgcgc
     1261 aatccagccc gtccgtcgtg gccttgcggc ggaaaacaag cacgatcccc atcgtgttta
     1321 cgatggtcac cgatccgatc ggccagggct ttgtcgaaaa cctggcgcgt ccaggcggta
     1381 acatcaccgg cttcagcgat ttcaacgcgc tgatggccgg caaatggctg gagatgctga
     1441 cacaggtcgc gccgcccgtc gcacgcgtgg ctgttctgta caatcccgaa accgcaccct
     1501 atgccggtct gatgatgcgc gccatcgatg acgcggcacc ctccttcgcc ataacggcac
     1561 aaagcgcgcc gtgccacgac gccgccgaga tccagtcaat gatgacggag cttgcgcgcg
     1621 aggagcgcgg cggattgctg gttttgacgg atattttcac catcgtgcat cgcgatacca
     1681 tccttgcttc cgccgcgcag caccgcctcc ctaccgtgta tttcgcgcgc tcgttcacca
     1741 cggccggcgg cctgatgtcc tatggcatcg actatgccga gctgttcctt cgctcggccg
     1801 cctatatcga tcgtattctg aagggcgcca gcccgcgcga cttgccagtc cagcagccaa
     1861 ccaagttcga actggtgatc aacctcaaga ccgccaaagc gcggggcatt agcctctcgg
     1921 caaagttgcc cgtcattccc cgccaccggg tgccgccttc ggcgagcccg atgacaggct
     1981 ccagtgggaa tccagtacgc cgcggcttca cggctctttc acaagcgctt ctggaatact
     2041 ggatcgcccg gtcaagccct acgtgattca cacatcgcca aaaccgaatc aggatcaatc
     2101 acttagatga ccatcgatgt tggaagcgca ggacgcagcg cgtagccacc aacgttgaaa
     2161 agactcgcgt tttccacctc gtcaatcgat gcccttc
//
LOCUS       DASWGM010000437         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2542796,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000437 DASWGM010000000
VERSION     DASWGM010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542796"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /gene="gltX"
                     /locus_tag="VGA15_12690"
     CDS             <1..1066
                     /gene="gltX"
                     /locus_tag="VGA15_12690"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX9588582.1"
                     /translation="RLYDGLWRDRDGSQAPSGMKPTIRLKAPQSGETVIEDQVQGRVV
                     WQNENLDDLVLLRGDGNPTYMLAVVVDDHDMGVTHVIRGDDHLINAARQKQIYDALGW
                     EIPSMSHIPLIHGPDGSKLSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIF
                     STAEMIADFDLSGVGRSAARFDFAKLENLNGHYIRHADDQSLVTMFEDVLDYVPDGSG
                     LKAKLNHTTRAQLLQAMPSLKERAKTLIELIAGSYFIFADRPLVIEPKAAALLTPENR
                     ELIGRLRFALEAVTNWTPETTEAAMRAFAEQNNLKLGAVAQPLRVALTGRTTSPGIFD
                     VLAVLGRQECLARLGDQAPS"
     gene            1220..>1796
                     /locus_tag="VGA15_12695"
     CDS             1220..>1796
                     /locus_tag="VGA15_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX9588583.1"
                     /translation="MDATSNTKTATLTVGNKNYDFPILSGTVGPDVIDIAKLYAQSGM
                     FTYDPGFTSTGSCQSKITYIDGDAGILEYRGYPIEQLAENGDFLETCYLLLYGELPTK
                     AQKEDFDSRVIHHTMVHEQLARFFQGFRRDAHPMAIMVAAVGALAAFYHDSTDITDPR
                     QRMIASMRMIAKVPTLAAMAYKYTIGQPFIYP"
BASE COUNT          371 a          584 c          514 g          327 t
ORIGIN      
        1 gcggctctat gacggcttgt ggcgcgaccg cgacgggtcg caagccccgt caggcatgaa
       61 gcccacgatc cggctgaagg cgccacaaag cggcgaaacc gtgatcgagg atcaggtcca
      121 gggccgcgtg gtctggcaga acgagaacct cgacgatctc gtcctgctgc gtggcgatgg
      181 caacccgacc tatatgctcg ccgtcgtggt cgacgaccac gacatgggcg tcacccacgt
      241 gatcagaggt gacgatcacc tgatcaatgc cgcacgccag aagcagatct acgatgcgct
      301 cgggtgggag attccgagca tgtcccacat tcccctgatc catgggcccg acggctcaaa
      361 gctctcaaaa cgccatgggg cattgggcgt cgatgcctac cgcgccatgg gatatttgcc
      421 ggcagcgctg cgcaattatc tggtcaggct cggctggagc cacggcgacc aggagatttt
      481 ttcgaccgcg gagatgatcg ccgatttcga cctgtcaggc gtaggccgtt cggccgcgcg
      541 cttcgatttc gccaagctgg aaaacctcaa cggccactat atccgccacg ccgacgatca
      601 atccctcgtg accatgttcg aagacgtgct ggactatgtc cccgacggtt ccgggctcaa
      661 ggccaagctc aaccacacca cgcgcgcgca attgctgcag gcgatgccga gcctcaagga
      721 gcgcgccaag acgctgatcg agctgatcgc cggttcctac ttcatttttg ccgaccggcc
      781 gctcgtgatc gagccaaaag cggcggcgct gctgacgccg gaaaaccgcg aactgatagg
      841 caggcttcgt tttgcgcttg aggcggtcac aaattggacc ccggaaacca ccgaagcggc
      901 catgcgggcc ttcgcggaac aaaacaacct caagctgggc gcggtggccc agccgctgcg
      961 ggtagcgctg accggacgta ccacctcgcc ggggatcttt gacgtgcttg cggtgctggg
     1021 acggcaggaa tgcctcgccc ggcttggcga tcaggcgccc tcataaggtg gtatcggaag
     1081 ccactgtctg cctatcttgc agcgcacaca gcaatgcgat acccatttaa gaggcgcttc
     1141 tagaattctc ggtttcccac gccatctgat ggcgtaccgg gccaccggct ttcgcaacga
     1201 tctcatcggg gacctcacga tggacgcaac atccaacacc aaaaccgcaa cgctgacggt
     1261 cggcaacaag aattacgatt tcccgatttt gagcggcacc gttgggcctg acgtgatcga
     1321 catcgccaag ctctacgctc agtccggaat gttcacctat gatcccggtt ttacctcgac
     1381 cggcagctgc cagtccaaga tcacctatat cgacggcgac gccggcatcc tggaataccg
     1441 cggctatccg atcgagcagc tcgccgaaaa cggcgacttc ctcgagacct gttacctcct
     1501 gctttacggc gagcttccaa ccaaggcgca aaaggaggat ttcgacagcc gcgtgatcca
     1561 tcacaccatg gtccacgagc agctggcgcg gttcttccag ggtttccgcc gcgacgccca
     1621 tccgatggcg atcatggtgg cggccgtcgg cgcgctggcc gcgttctatc acgactccac
     1681 cgacatcacc gatccgcggc agcgcatgat cgcttcgatg cggatgatcg caaaagtccc
     1741 gacgctcgcc gcgatggcct acaaatacac catcggccag cccttcatat atccga
//
LOCUS       DASWGM010000438         9854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_167795,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000438 DASWGM010000000
VERSION     DASWGM010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167795"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..734
                     /locus_tag="VGA15_12700"
     CDS             195..734
                     /locus_tag="VGA15_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9588584.1"
                     /translation="MRRLMLLRHAKTEHDAPSGRDRDRRLDERGRTDAAKIGGWISQH
                     PPFPDLVLVSPAIRARQTWEIVWEAMKNLSPQPQVELPPELYGADPSRLLQTVHASSS
                     NPKVLMLVGHNPGMHELALALTGSGEAAARKALADNLPTSGLAVFDFAGGDWTDVAFR
                     RGRLVLFVTPKGLRQTSGE"
     gene            803..1228
                     /locus_tag="VGA15_12705"
     CDS             803..1228
                     /locus_tag="VGA15_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9588585.1"
                     /translation="MFKSILVPIDLSDTELAKPAIATAATLSQTWSGTVRLLNVLPMT
                     PVMLAEYVPADFDAQQRQASEEALAIVARESGIDAQRISSVVRQGGIYHEILEEAAAI
                     HADLIVMTSHRPAMRTYFLGSNAGHVVRYAKCSVLVVRH"
     gene            complement(1477..2391)
                     /locus_tag="VGA15_12710"
     CDS             complement(1477..2391)
                     /locus_tag="VGA15_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX9588586.1"
                     /translation="MQRRYITVDVFTDRAFGGNPLAVVLDASGLSTAQMQAIAVEFNY
                     SETTFVLPPRDAAHDAQVRIFTVTTEMPFAGHPNVGTAFVLATQAAKPPARLKFEEGA
                     GLVPVEILTEGGRVVGAELTAPQPLSRLTEFSAEQAAAAVALSAADIRSDQHPAMIVS
                     VGMPFLVVELGSRVALRRARPDAATFERTFPCDGALAIYLYTRDVPPDEKATDLQARM
                     FFPGSSGLTEDPATGSATVAAAALLADLSGERDGELKLRIGQGVDMGRPSLLLTRVRK
                     ENGVVVSAHVGGGCVQMMEGTFRLAGEG"
     gene            complement(2472..2756)
                     /locus_tag="VGA15_12715"
     CDS             complement(2472..2756)
                     /locus_tag="VGA15_12715"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX9588587.1"
                     /translation="MTVIFSGTARRATSGRSGGFVRWIGICASALAARWERRAAINAL
                     RGRDDRELRDIGIARCQIEAAVGGALNPAMGRLDESHRCRSGKPPVDRES"
     gene            2940..4283
                     /locus_tag="VGA15_12720"
     CDS             2940..4283
                     /locus_tag="VGA15_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX9588588.1"
                     /translation="MSKFEYVKLADAVAAEIADGSMKPGDRLPPQRTFAYERKIAVST
                     ASRVYTELLRRGLVVGEVGRGTFISGEAKRGIAAPSEPRGARIDLEFNYPTLPMQSAL
                     IAKSLEGLDRPEELDIALRQAASVGTPNARNISAIFLARQNWSPKPEQLVFTASGRQC
                     IAAALAAVVPIGGRCGVEALTYPFIKGIAARLGVTLVPLAMDDAGVRPDSVQKAHREG
                     HLSALYVQPVIQNPLGMTMPSSRRADLLRVVEKLGLVVIEDAVYGFLDDVPPLAALSP
                     ESCIVLDSLSKKVAPGLALGFIVPPLRLRESIMASVRSGGWTAPGYAFAAGQRLMSDG
                     TASELSRLKRIDARQRQKIATACLSGFEIQTNSKSYHLWLTLPPHWRSQTFVAAAARR
                     DIALTPSMTFAVTPGHAPNAIRLGLAAPAMEQLEAGLRTLSGMLNAKEEDFDSTE"
     gene            complement(4315..4905)
                     /locus_tag="VGA15_12725"
     CDS             complement(4315..4905)
                     /locus_tag="VGA15_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX9588589.1"
                     /translation="MEDRHTRRLTEAEIAEISARHFVATPIKPADLLFVFGTRGDVGR
                     RVEEAYRLWREGLFCWSIVSGGVTPGSTLSECEIIKTAMVERGIPPDCILEEHRARNT
                     GENVIFSLPVLEAALGLANIRSVICLGNTWTARRYPMTLHRHWPEVEKMLITVDSFET
                     PLALWHTDPDFRIRVLAEWDKIEPYKARGFIAEWPE"
     gene            complement(5006..5596)
                     /locus_tag="VGA15_12730"
     CDS             complement(5006..5596)
                     /locus_tag="VGA15_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588590.1"
                     /translation="MTTDLSDGTATNVCYAYKASLIGSAHQFELTDDGLSWRVGGKSG
                     VWAYADIAAIRLSYRPVSMQSRRFRADIESANRQKITVLSTSWQTVALMAPQDRDYRA
                     FVTQLHQRMKEAGSKAALIGGVGPKTYAAGIVLLALVGIAISGLFIRAIATSEFSGAL
                     FLVGFAALFTWQIGGFVARNRPRAYTFDHLPETLLP"
     gene            complement(5654..6088)
                     /locus_tag="VGA15_12735"
     CDS             complement(5654..6088)
                     /locus_tag="VGA15_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEX9588591.1"
                     /translation="MTIGLLALTVAAIFTGAALYVGVAEQPARLLLDDRALLTEWKPS
                     YKRGAAMQASIALAGFLLGLIAWWQTSHSGFLIGAIAMIAPWPWTLIGIKPTKDALLA
                     TEPDQAGPSTRALVVKWGALHAVRTALGALATLAYLWASLSR"
     gene            6188..6622
                     /locus_tag="VGA15_12740"
     CDS             6188..6622
                     /locus_tag="VGA15_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588592.1"
                     /translation="MAETTYFPRRLILAGAMISGVLLALAVHMLGARYGLDLGGLWRS
                     DTGEFMPAGAAVAWWLIATVGFSGGYFTATLMASAVSGQIPQRMRQFLIGVGVLVLAG
                     AGQAAAAPSPIPTISGVLAGLAALCLGALMAFCGAHFALRKA"
     gene            complement(6658..8670)
                     /locus_tag="VGA15_12745"
     CDS             complement(6658..8670)
                     /locus_tag="VGA15_12745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9588593.1"
                     /translation="MSAAPIDRRDFLRRSAALGTALPFLGFAGNAAAQQADLVARRVY
                     FDNPDYGSVRVSPDGQTLAFLAPIDGVRNLWVTPVADLAAARPVTRATDRNLAAYFRW
                     AHTNRHLIYFQERDGDENWRASSVDIGSGAIVPLSPERGVKSFLQEVDHKFPDQMLLR
                     HNARNKRYFDLFRVNVVTGASELLYENNEYVLLITDSDFQLRLSSRIATDGTSEYFER
                     RGDGNWTPFTSVPIGDTDATRMLDFSADGKTLHMLDSRERDKAALFAVDMATRKATLL
                     AADDEADIVEVSFDDQRRPIAARSNRDRAHWCVVDPGAAQDLADLAGHGPGDVAIIER
                     SADNRMVTVFYERDAESGEYALLDRQKREVRSLFRQRKALDGVALRMMQPVVIPARDG
                     LRLNGYLTLPATEAGAGKLPMVLLIHGGPYARDQWGFNSTHQWLANRGYAVLSVNYRG
                     STGFGKAFVAAADREWGGRMHDDLIDAVDWAVAHDIADPKRVGFFGASYGGYSALMAA
                     TKTPEIFACIVDMFGISNLMTFMATIPPYWGPWISVWKNRLGDPGTEQGRAFLTERSP
                     INHLERATRPILVAQGMQDVRVVAAESEQMVTALKNRGVPVTYITFSDEGHGFARPEN
                     RLAFYGVAEAFLAKHLGGRSRPVGNDFSGSTLKVETGGELVPGLSG"
     gene            complement(8763..>9854)
                     /locus_tag="VGA15_12750"
     CDS             complement(8763..>9854)
                     /locus_tag="VGA15_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9588594.1"
                     /translation="VACALAPNAAILIAARAVQGLGAAILVPNSLALLSHAYPDEKQR
                     GRAVGIWAAGASLALISGPLIGGGLIALIGWRSIFLVNLPIGLAGLWLSWRYATETTR
                     HQQREVDLPGQAAAIAALGCLAGAIIEGGTLGWRDPFVIAGLAASAVLAILFVVQERR
                     APQPMLPLSLFRHRLFALTALVGLLVNVAFYRLIFVFSLYFQRVNGLSPFATGLAFLP
                     MLGAVLPVNLVAARVAERIGAPATIAVGNAMAACGCLALLGIERGTSYWAIGAQLTII
                     GAGLGLLVPPLTSTLLGSVEKSRSGIAAGALNSTRQTGSVLGVALFGSLAGQANAFMA
                     GTHWSLIISACLLLAAGAVIWFGVSMKDS"
BASE COUNT         1877 a         3184 c         3045 g         1748 t
ORIGIN      
        1 cacacagata tcgacgtcca gatcgaacga aaggcgcgag cgctctcgca ggtcggcgcc
       61 atcggatctt gcggccatgc ctaaagaagt ctcactcata ccggtttctt acggacggat
      121 cagtcgcttg tcaccttgtc ccgagcttca gcttgtacat taatccgcaa gacccgaaac
      181 gaatcgagat cgccatgcgc cgtttgatgc tgctgcgcca cgccaagacc gagcacgacg
      241 cgccgagcgg ccgcgatcgg gaccgccgat tggacgaacg cggccgcacc gatgccgcga
      301 agatcggcgg ctggatcagc cagcacccgc cttttcccga tttggtgctg gtttcgcccg
      361 cgatccgggc ccgccagacc tgggagattg tctgggaggc gatgaagaac ttgagccccc
      421 agccgcaggt tgaacttccg cctgagcttt atggagccga cccgtcgcgg ctcctgcaga
      481 ccgtccacgc atcatcatcc aatccgaaag tcctgatgct ggtcgggcac aatcccggaa
      541 tgcacgaact ggcgcttgcg cttactggca gcggtgaagc cgccgcacgc aaggcgctcg
      601 ccgacaacct gccgacatcg gggcttgcgg tattcgactt tgcgggcggc gactggaccg
      661 acgtggcctt ccgtcgcgga cggctggtgc tgttcgtcac cccgaagggg ctcaggcaaa
      721 cctccggcga gtaagtggaa ccaactgccg cgtcgcccta accaggtttt atgctacgtt
      781 gacgtcacac acggaggcgc agatgttcaa atccattctc gtgccgatcg atctctccga
      841 taccgaactg gcgaaacccg cgattgcgac ggcagcgacg ctgtcgcaga cctggagcgg
      901 cacagtgcgg cttctgaatg tgttgccgat gacgccggtg atgctggcag aatatgtgcc
      961 cgccgatttc gacgcccagc agcgccaggc ctccgaggag gcactcgcca tcgtggcccg
     1021 cgaatcaggc atcgatgccc agcgcatctc cagcgtggtg cgccagggcg gcatctatca
     1081 tgagattctc gaagaggccg cggctataca tgccgacctg atcgtcatga catcgcaccg
     1141 gccggcgatg cggacctatt ttctcggctc caacgccggc catgtggtac gctatgccaa
     1201 atgctcggtg ctggtggtcc ggcactagag cgttttcgag cgaaagcctg ccccgggctt
     1261 gatccggggt ggagaccggt tcgcgtcaag aaaacgcgtc aaaccaagaa tctagagccc
     1321 cgtttcgatt ctatcgaaac ggaagaggct ctagcgatac cggcttcaga tctaaagact
     1381 gtcattccgg ggcgcgtgaa gcgtgagccc ggaattcata accacgatcg ggagtatgga
     1441 ttctgggcct gcgccaagcg gcggcgacct cggccttcac ccctcgcccg ccagccgaaa
     1501 cgttccttcc atcatctgca cgcaaccgcc gccgacatgc gctgaaacca caacgccgtt
     1561 ttctttgcgg acgcgggtca gcaacaggct tggccgcccc atgtcgacac cctgccctat
     1621 gcgcagcttc aactcgccgt cccgctcacc ggacaaatcg gcaagcagcg cggcggcggc
     1681 cacggtggcg ctgccggtgg cgggatcttc ggtgaggccg ctcgatcccg gaaaaaacat
     1741 ccgcgcctgc aggtcggttg ccttctcgtc cggcggcacg tcgcgcgtat agaggtagat
     1801 cgcaagggcc ccatcgcatg ggaatgtccg ctcaaatgtc gcagcgtcag gcctggcgcg
     1861 ccgtagcgca acgcgcgaac ccaactccac caccaggaac ggcattccga ccgagacgat
     1921 catggctgga tgctggtcgc tcctgatatc cgcggccgac agcgctaccg cggccgccgc
     1981 ctgctcggcg ctgaactccg tcagtcggct gagcggttgc ggcgcggtga gttcggcgcc
     2041 gacgaccctg ccgccttccg tcaggatttc gactggcacc agaccggcgc cttcctcgaa
     2101 cttcagccgc gccggcggtt ttgcagcctg cgtcgccagc acgaaggcgg taccgacatt
     2161 gggatgtccc gcgaacggca tttcggtggt cacggtaaag atacgcacct gcgcgtcatg
     2221 cgcggcatcg cgcggcggca acacgaaggt ggtctcggaa taattgaact cgaccgcaat
     2281 ggcctgcatc tgcgcggtgg agagcccgct ggcgtccagc accaccgcaa gcggattgcc
     2341 gccgaaggcg cggtcggtga aaacgtccac ggtgatatag cgacgttgca tgagagatct
     2401 ccggttcggt aatcaacgtg atcagtgtcg cgcccagtct gttcctgtcg cgcgacatga
     2461 ttgagctggc ttcagctttc gcgatctacc ggaggcttac ccgatcgaca ccggtggctt
     2521 tcatcgagcc ttcccatcgc tggattcaag gccccgccga ccgcggcctc gatctggcag
     2581 cgagcgatcc cgatgtcgcg caattcacgg tcgtcccgtc ctcgcagcgc gttgatcgca
     2641 gcccggcgct cccaacgggc cgccagggcg gatgcacaaa ttccgatcca gcggacaaat
     2701 ccgcccgacc tgccggaggt cgcacgtcgt gcggtgccag agaaaatcac ggtcatatcg
     2761 gttctccaat cggcttccgt ccggcaaagc gatgccgttg gcgcaaacgc tggtcgagcg
     2821 cggcagcacc tccgcgacga aagattgctc tcgcttccct cagtgcaatg tcgcgttgat
     2881 tgccaagaaa taatctgtaa ttgtatcggt tcaagcaaaa cattgctctc ggtgcaataa
     2941 tgtcgaaatt cgaatatgtg aagttggccg acgccgttgc cgccgaaatc gccgacggct
     3001 cgatgaagcc gggcgatcgc ctgccgccgc agcgcacctt cgcctacgag cgcaagatcg
     3061 ccgtctcaac cgcaagccgg gtttacacgg agctgctgcg tcgcggcctg gtcgtcggtg
     3121 aggtgggacg cggcaccttt atttcgggag aagcgaagcg cgggatagcc gcgccgagcg
     3181 aaccgcgagg agcccgcatc gatctggaat tcaattatcc aactctgccg atgcaatcgg
     3241 ccctgatcgc aaagagcctg gagggattag acaggccgga agaactcgat atcgcgctga
     3301 gacaagcggc gagcgtcgga acaccaaatg cgagaaacat ctccgcgatt tttctcgccc
     3361 gtcaaaattg gtctccaaaa cccgagcaac tggtattcac cgccagcggc cggcaatgca
     3421 tcgcagccgc gcttgccgcg gtggtgccga tcggcggccg gtgcggcgtc gaggcgctga
     3481 cgtatccctt catcaagggc atcgccgccc ggctcggcgt gacgctggtg ccattggcga
     3541 tggatgatgc aggcgtccgg ccggactcgg tgcaaaaggc tcatcgcgag gggcatctat
     3601 cggccttgta cgttcagcct gtgattcaaa atccgctggg catgacgatg ccatcgtcgc
     3661 gccgtgccga tctcttgcga gtggttgaaa agctcggcct cgttgtcatt gaggacgcgg
     3721 tttacggctt tctcgatgac gtaccgccgc tcgccgcgct ctcgccggaa tcatgcatcg
     3781 tgcttgatag cctttccaag aaagtcgcgc ccgggctggc gctcggcttt atcgtccccc
     3841 cgcttcgctt gcgcgaaagt atcatggcct ccgtgcgttc tggcggatgg accgcgccgg
     3901 gatatgcgtt cgcggccggg caacggctga tgagtgacgg caccgcctcc gaactttcaa
     3961 gacttaagcg gatcgatgct cggcaacgtc agaaaatcgc gaccgcctgc ctgtccggct
     4021 tcgagatcca gaccaacagc aaatcgtatc acctgtggct gacgctgccg ccgcactggc
     4081 ggtctcaaac ctttgtcgcc gccgctgccc ggcgagatat cgctttgacc ccctcaatga
     4141 ccttcgcggt gacccctgga catgcgccga atgccatacg gttgggcttg gccgcgcccg
     4201 cgatggagca gctcgaggcg gggctgcgaa cgctgtcggg aatgttgaac gcaaaggaag
     4261 aggatttcga ttccactgaa tagcggtgct ggggccgatc acccctcggc tcgttcactc
     4321 cggccactcc gcgataaaac ccctggcctt gtagggctcg atcttgtccc attcggcgag
     4381 aacccggatc cggaaatccg gatcggtatg ccagagcgcc agcggcgttt caaaactgtc
     4441 gaccgtgatc agcatcttct ctacctccgg ccaatgccgg tgcagcgtca tcgggtagcg
     4501 gcgcgcggtc caggtattgc cgaggcagat gacgctgcgg atattcgcaa ggccgagggc
     4561 ggcttcgagg acgggcagcg agaagatgac gttttcgccg gtgttcctcg cgcgatgttc
     4621 ttccaggatg cagtcaggcg gaatgccccg ctcaaccatc gcggtcttga tgatctcgca
     4681 ttccgaaagc gtcgagcctg gcgtcacccc gccgctcacg atcgaccagc aaaacaagcc
     4741 ctcgcgccaa agccggtaag cttcctcgac acgccggccg acatccccgc gggtgccgaa
     4801 cacgaacagc aaatcggcgg gtttgatcgg tgtcgcgaca aaatgcctgg cgctgatctc
     4861 ggctatttcg gcctcggtca gccgtcgcgt gtgtcgatcc tccacgtccg cctcgcatgc
     4921 aatgctgaat caggcaccgc taccatgcgg cgcgacggcc gcgacgcgaa gtcacgtttc
     4981 attgcgcttt tttgcagagg caggattacg gcaacagcgt ttccggcaga tgatcgaacg
     5041 tgtaggcccg cggcctgttg cgtgccacaa agccgccgat ctgccacgta aacagcgcgg
     5101 cgaagccgac cagaaacaac gcgcccgaaa attcgctggt ggcgatggcg cgaatgaaga
     5161 gtcccgatat cgcgatcccg accagcgcca gcaacacgat gccggcggca taggttttcg
     5221 gcccgacgcc cccgatcagg gcggccttgc ttccggcctc cttcatgcgc tgatgcagct
     5281 gcgtgacaaa ggcgcggtaa tcccgatcct gcggcgccat caaggcgacg gtctgccagc
     5341 tggtcgagag gacagtgatt ttctgacggt tcgcagattc gatgtcagcg cgaaaccgcc
     5401 gcgattgcat cgacaccggc cggtaggaca gacggatcgc ggcgatatcg gcataagccc
     5461 agaccccgga cttgccgccg acccgccacg acaatccatc gtcggtcagc tcgaattgat
     5521 gcgccgagcc gatcagcgac gccttgtagg cgtagcagac gttggtggca gtcccgtccg
     5581 acaaatcggt cgtcataggc aatgtgctga tccctcggga tagacgccgg tgcacgggaa
     5641 tcgtatgtcg cggctagcgc gatagagatg cccacaaata ggcaagtgtt gcgagcgcgc
     5701 ccaatgcggt gcgcacggca tggagtgcgc cccatttgac gacaagcgcg cgcgtcgacg
     5761 gccccgcttg atctggttcg gtcgccagca gcgcatcctt ggtgggtttg atgccgatca
     5821 gcgtccacgg ccacggcgcg atcatcgcaa tggcgccaat cagaaagccg gaatgcgagg
     5881 tctgccacca cgcgatcaac cccaaaagaa aacccgctag cgcgatcgac gcctgcatcg
     5941 cggccccgcg cttgtaggac ggcttccatt cggtgagcag cgcgcggtcg tcgagcaaca
     6001 gccgcgccgg ctgctcggcg acgccaacat aaagcgccgc cccggtgaag atcgcggcga
     6061 ccgtcagcgc gagaagaccg atcgtcatgt gccgagcctt tcctaaaagg gattgcttga
     6121 tcaaaagatt gcttgaactc tccaccgcgg cttgcgcggc gagccggctt taccttacaa
     6181 gcaggccatg gccgagacga cctattttcc gcgccgcctg atcctcgccg gcgcgatgat
     6241 ttcgggagtg ctgctggcgc tcgccgtgca catgctcggc gcacgctacg gcctcgacct
     6301 cggcggcctg tggcgatccg ataccggcga attcatgccc gcgggcgccg ctgtggcgtg
     6361 gtggctgatc gcgacggtcg gcttttccgg cggctatttc accgccacac tgatggccag
     6421 cgcggtgtcg gggcaaattc cgcagcgaat gcggcagttc ctgatcggcg tcggggtgct
     6481 ggtgctcgcg ggcgcgggcc aggcggccgc agcacctagt ccgatcccga cgatatccgg
     6541 cgtgctggca ggtctcgcag cgctatgcct tggcgcgctg atggcattct gcggcgcgca
     6601 ttttgccctg cgcaaggcgt gaactggcgc attcgcgttt gagccggcca cacccaatta
     6661 tccggagagt ccgggcacca actcgccgcc ggtttcgacc ttgagcgtcg agccggaaaa
     6721 atcgtttcca accggccggg aacgaccgcc gagatgcttc gccagaaacg cctcggccac
     6781 gccatagaag gcaagccggt tttccggccg ggcgaaaccg tgcccctcgt cggaaaaggt
     6841 gatgtaggtg accggcacgc cgcggttctt cagcgccgtc accatctgct ccgattccgc
     6901 cgcaacgacc ctgacgtcct gcatgccctg ggcgaccagg atcgggcggg tcgcgcgttc
     6961 gagatggttg atcggcgagc gctcggtgag aaaggcgcgg ccttgttcgg taccggggtc
     7021 gccgagccgg tttttccaga cgctgatcca gggaccccaa tagggcggaa tcgtcgccat
     7081 gaaggtcatc agattggaga tgccgaacat gtcgacgatg caggcgaata tttctggcgt
     7141 cttggttgct gccatcaacg ccgagtaacc gccatagctc gcgccaaaga acccgacgcg
     7201 cttgggatcg gcgatatcat gcgcgaccgc ccaatcgacc gcatcgatca gatcgtcgtg
     7261 catccgccca ccccattcgc ggtcggcggc ggcgacaaac gctttgccga atccggtcga
     7321 gccccggtag ttgacgctga gcaccgcata gccccggttt gcgagccatt gatgggtgga
     7381 attgaagccc cattgatcgc gcgcataggg cccgccatgg atcaacaaca ccatcggcag
     7441 cttgccggcc cctgcttccg ttgccggcaa tgtcagataa ccgttgaggc gaagtccgtc
     7501 gcgcgccgga atgaccaccg gctgcatcat tcgcaacgcc acgccatcga gcgccttgcg
     7561 ctgcctgaac aagctgcgca cttcgcgctt ttgacgatcg agcagcgcat attcgccgct
     7621 ctcggcatcg cgttcgtaaa acaccgttac catgcgattg tcggcactgc gctcgatgat
     7681 ggcgacgtcg cctggtccgt gcccggccag atccgccaga tcttgcgccg cgcctggatc
     7741 gacgacgcac cagtgcgcac gatctctatt cgatcgcgcg gcgatcggac gacgctggtc
     7801 gtcgaaggag acctcgacaa tgtcggcttc atcgtcggcg gcgagcaagg tggctttgcg
     7861 ggtcgccatg tcgacggcga aaagtgcggc tttgtctcgc tcccgcgagt cgagcatatg
     7921 cagcgtcttg ccgtcggcgc tgaagtcgag catccgggtg gcatcggtat cgccgatcgg
     7981 cacgctggtg aatggtgtcc agttcccgtc gccgcgtcgc tcgaaatatt ccgacgtgcc
     8041 gtcggttgcg atgcgcgaac tcaaccgcag ttgaaagtcg ctgtcggtga tcagcaagac
     8101 gtattcgtta ttttcgtaaa gcagctcgct cgccccggtc acgacgttga cccgaaacag
     8161 gtcgaaatat cgcttgttgc gcgcgttgtg ccgcagcagc atctgatcgg gaaatttgtg
     8221 atccacttcc tgcaggaacg acttgacccc gcgctccggg ctcagcggca cgatggcgcc
     8281 gctgccgatg tcgacgctcg aggcgcgcca gttctcgtca ccgtcgcgct cctggaaata
     8341 gatcagatgg cgattggtgt gcgcccagcg gaagtaagcc gccagattgc gatcggtggc
     8401 gcgcgtcacg ggtcgtgccg cggcaagatc ggcgaccggc gtcacccaaa gattgcggac
     8461 gccgtcgatc ggcgcgagaa aagccagggt ttggccgtcg ggacttactc ttacgctgcc
     8521 gtaatcgggg ttgtcgaaat agacccgccg ggcgacgaga tccgcctgct gcgcagcagc
     8581 gttccccgcg aagcccaaga agggcagcgc cgtgcccaag gcggcgctgc ggcgcagaaa
     8641 gtcgcggcgg tcgatcggag cggcggacat gcgaacctcc gtctaagcgg ccgcgcttgt
     8701 gccggcccgc ttcgcaaccc tagatgcgac gagcgatatc cgcaacaaac tgccccggat
     8761 cattacgaat ctttcatgga aaccccaaac cagatcactg ctccggctgc gagcagcagg
     8821 cacgccgaga tgatcagcga ccaatgcgtg cccgccatga aggcgttggc ctgaccggcc
     8881 agcgagccga acagcgcgac acccagcacg ctgccggttt gccgggtcga gttcagtgcg
     8941 ccggcggcga tgccggagcg cgacttctcg acgcttccga gcagcgtcga tgtcagcggc
     9001 ggcaccagca ggccaagacc ggcgccaatg atggtcagtt gcgcgccgat tgcccagtag
     9061 ctggtgccac gctcgatccc gagcagcgcc agacatccgc atgccgccat cgcattgccc
     9121 accgcgatcg tcgcgggcgc accgatgcgc tcggccacgc gcgcggcgac gaggttgacc
     9181 ggcaacaccg ctcccaacat cggcaggaac gcaaggccgg tcgcaaaggg cgacaatccg
     9241 ttgacgcgct ggaagtacag gctgaaaacg aagatcaatc gatagaacgc gacgttgacg
     9301 agaaggccga ccagcgccgt cagcgcgaac agccgatgcc tgaacagcga cagcggcagc
     9361 atcggttgtg gcgcgcgccg ttcctgcaca acgaacagga tcgccagcac cgccgaggca
     9421 gccaagcccg cgatcacgaa cggatcgcgc cagccaagcg tgccgccttc gatgatcgcg
     9481 ccggcaagac aaccaagtgc tgcgatcgcc gcggcctggc ccggcaaatc gacctcgcgc
     9541 tgctgatgtc gcgtggtctc ggtggcatag cgccagctca accacaggcc ggcgagaccg
     9601 atcggcagat tgaccaggaa gatcgaccgc cagccaatca acgcaatcag gccgccgcca
     9661 atcaacggcc cggagatcag tgcgaggctg gcaccggcgg cccaaattcc caccgcgcga
     9721 ccgcgctgtt tttcgtccgg gtaagcgtgg ctgagcagcg caagggagtt gggaacgaga
     9781 atggcggcac cgagtccctg gaccgctctt gccgcgatca ggatcgcggc attgggcgcg
     9841 agcgcacagg ccac
//
LOCUS       DASWGM010000439         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2123875,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000439 DASWGM010000000
VERSION     DASWGM010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123875"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VGA15_12755"
     CDS             complement(<1..1271)
                     /locus_tag="VGA15_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9588595.1"
                     /translation="MTKPIETENSKPAPSAPRRPHSFTTHGITVVDDYAWLKDANWQE
                     VLRDPAGLDADIRNYLEAENAYTESLLGHTAGLQKTLVKEMRGRIKEDDSSVPAPDGA
                     YAYLRKFRDGGEHEMFGRTQRDGGEIEIVLDGDRLAADHEYFKFGGSRHSPDHKLEAW
                     SADIKGSEYFTIRVRDWKTREDSADLVEETDGGVVWNADATAFYYVKLDDNHRPMQVF
                     RHRLGTPQADDVMIHEEKDAAWFTHIHESASGRFCVIAGGDHETSEQWLIDLAREEAK
                     PRLVAARETGVQYSIADRGDELFILTNAEGAIDFKIVTAPLKSPERANWRDLIPYREG
                     VYIIDIELYAGHLVRLERANALPSIVIRDLKTSDEHAIAFDETAYSLDTMGGYEFDTT
                     NLRFSYSSMTTPSEVYDYDMASRARILRKRQ"
     gene            complement(1362..>2165)
                     /locus_tag="VGA15_12760"
     CDS             complement(1362..>2165)
                     /locus_tag="VGA15_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9588596.1"
                     /translation="WRWAALAFCLVVLLNPVFLPYLALLNGALSPNATTLVTPSTLTM
                     HNIVFVFTELSSTQLALKNTVILGTATATIGTILALVIAYVTTRRVIAGHRVLGFLAT
                     APVAVPGIVLGVGLFLSYTRPPFVLYGTLWILLIAFLTINLPSAYQQLQAAFATIHPE
                     LEDASRILGATRLRSLLQITAPLLRTGVIATWCFIFIGVMRELSAAIVLFTSQTKVLS
                     VLIYDLNESGDLAAISVLGIAMLIITFAVVLAVNQIPVFGGSAGARLRN"
BASE COUNT          409 a          650 c          688 g          418 t
ORIGIN      
        1 tcctgacgct tgcgcaagat tcgtgcgcgg ctcgccatgt cgtaatcgta gacttccgac
       61 ggcgtcgtca tcgacgaata cgaaaaccgc aaattcgtgg tgtcgaattc atagccgccc
      121 atggtgtcga gcgaatacgc cgtctcgtcg aaggcgatgg cgtgctcgtc actggttttg
      181 agatcgcgga tcacgatcga gggcagggcg ttggcgcgct ccagccgcac caggtggccg
      241 gcgtagagtt cgatgtcgat gatatagacg ccttcacggt acgggatcag gtcgcgccag
      301 ttcgcgcgct cgggcgactt gagcggcgcg gtgacgatct tgaagtcgat cgcgccctcg
      361 gcattggtga gaatgaacaa ttcgtcgccg cggtcggcga tcgagtattg cacgccggtt
      421 tcgcgcgccg ccaccagccg cggttttgct tcttcacggg caagatcgat cagccattgt
      481 tccgacgttt cgtggtcgcc gccggcgatc acgcagaacc ggccgctggc gctttcatgg
      541 atatgggtga accatgcagc atccttttcc tcatggatca tcacatcgtc cgcctgcggc
      601 gtgccgaggc gatgccggaa aacctgcatc gggcgatggt tgtcgtcgag cttcacataa
      661 tagaatgccg tcgcgtccgc gttccagacc acgccgccat cggtttcctc gacaaggtcg
      721 gcgctgtcct cgcgggtttt ccagtcgcgg acgcggatcg tgaaatattc cgagcccttg
      781 atatccgcgc tccaggcttc cagcttgtga tccggcgaat gccggctgcc accgaacttg
      841 aaatattcat ggtcggctgc aagcctgtcg ccatcgagca cgatctcgat ctcgccgccg
      901 tcgcgctggg tacggccaaa catctcatgc tcgccgccgt cgcggaattt gcgcaaatac
      961 gcgtaagcgc cgtccggcgc gggcacgctg gaatcgtcct ccttgatccg gccgcgcatt
     1021 tctttcacca gcgtcttttg caggccggcg gtgtggccga gcaggctctc ggtataggcg
     1081 ttttcggctt cgaggtagtt tcggatatcc gcgtccagac ctgcgggatc tcgcagcacc
     1141 tcctgccagt tggcatcctt cagccacgca taatcgtcga caacggtaat gccgtgggtg
     1201 gtgaatgaat gcggccgccg cggcgcggat ggcgcgggct ttgaattctc tgtttctatt
     1261 ggttttgtca cttgggtcct cgttcgcagc ggacaccgcc gcagccccca atatcggttc
     1321 gatccagtgg attgccagag gtgccgcgaa gcgcggcaga gttaattgcg cagccgcgcg
     1381 ccggcgctgc cgccgaacac cggaatctgg ttgacggcca gcaccaccgc gaaggtgatg
     1441 atcagcatgg cgataccaag caccgaaatc gcggcgaggt cgccgctttc attgaggtcg
     1501 tagatcagca cgctgagcac cttggtctgc gacgtgaaca gcacgatcgc ggcggatagt
     1561 tcgcgcatga cgccgatgaa aataaagcac caggtcgcga tcacgccggt gcgtaatagc
     1621 ggcgcggtga tctggagcag cgaccgcagc cgcgtcgcgc cgagaatccg gctggcatcc
     1681 tcgagttcgg gatggatggt ggcgaaggcc gcctgcaatt gctgatacgc cgacggcagg
     1741 ttgatggtga gaaacgcgat cagcaggatc cacagcgtgc catacaatac gaatggcggc
     1801 cgggtgtagc tcaggaacaa gccgacaccc agcacgatgc cgggaacggc gaccggcgcg
     1861 gtggcgagaa agccgagcac gcgatgcccc gcgatcacgc ggcgcgtggt gacgtaggcg
     1921 atgaccagtg cgagaatggt gccgatggtc gcggtcgcgg tgccgagtat caccgtgttc
     1981 ttgagcgcaa gctgcgtcga cgacaactcg gtaaacacga acacgatgtt atgcatcgtc
     2041 aacgtcgacg gcgtgaccag cgttgtggcg ttcggcgaga gcgcgccgtt cagcagcgcc
     2101 agatacggca ggaacaccgg attgagcagc accacgaggc agaacgccaa cgccgcccag
     2161 cgcca
//
LOCUS       DASWGM010000440         1712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2404908,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000440 DASWGM010000000
VERSION     DASWGM010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404908"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1346
                     /gene="hpnI"
                     /locus_tag="VGA15_12765"
     CDS             204..1346
                     /gene="hpnI"
                     /locus_tag="VGA15_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEX9588597.1"
                     /translation="MIIQWLSDFCFLAACIGCLYLATAAAMVLRFRGHRPSTNGQPLP
                     VTILKPLHGEGPRLFRCLASFCSQRYPAPVQIVFGVEEAADAAIPLVKQLQAAFPESK
                     IELRIDPRAHGGNRKISNLANMAALADHDIVILADSDIEVDPDYLARIVGELQQPGIG
                     AVTCLYHGMSGTRTWARLAALAANAQFLPSVIVGVACRLAQPCFGATIALRRDMLVRI
                     GGFEQFADSLADDYSIGEAVRAMGAEVAISAVSVGHLCKDQTARQLCSHELRWGRTIR
                     SIDPVGYFSSIITHPFPLALLAALLGAGGGAVALAVLAVICRVTLLKCVERRFSLERQ
                     PYWLVPLRDLISFVIFVWSLFGTSVDWNGETYQVTSQGILVRRTAV"
     gene            1346..>1712
                     /locus_tag="VGA15_12770"
     CDS             1346..>1712
                     /locus_tag="VGA15_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEX9588598.1"
                     /translation="MRTLFLQAPSFEGFDGGAGSRYQARREIRSFWYPTWLAQPAALV
                     PGSRLIDAPSHGVSLNEIAAQADRFDLAVLHTSAPSFAADLAAIEALKRGNPRLKAGL
                     IGAKVAVDPEGSLKASAAVD"
BASE COUNT          280 a          561 c          538 g          333 t
ORIGIN      
        1 ctacggcacc ccgcaaaaat tggagggggt cgggaattcg acttgcgaac gcctcatttc
       61 aatgccccaa tcgatggcac cttccccctc gctagcgccc gattgcggcg agctatccac
      121 ctcggcacga cagacccggc gcggaactcc agaaaacgcg gaacatggat tgagctggca
      181 cattagctcc gcgcggtaac cctatgatca ttcagtggct atcggacttt tgcttcttgg
      241 cggcctgtat cggctgcctc tatcttgcga ctgcagccgc catggtgctg cgcttccgcg
      301 gccaccggcc gtccacgaat ggccagccgt tgccggtgac cattctcaag cccctgcatg
      361 gagagggtcc ccgacttttc cggtgtctcg cctcgttctg cagccagcgt tacccggcgc
      421 cggtgcagat cgtgttcggg gtcgaggaag ccgccgacgc cgcgatcccc ctggtgaaac
      481 agctgcaagc ggcatttccc gaaagcaaga tcgaactcag gatcgacccg cgcgcgcacg
      541 gcggcaaccg caaaatttcg aatttggcaa acatggccgc tcttgccgac catgacatcg
      601 tgatcctggc cgacagcgat atcgaggtcg atcccgacta cctcgcgcgg atcgtcggcg
      661 agttgcaaca gccggggatc ggcgccgtga cctgcctcta tcacggaatg tccgggacgc
      721 gcacctgggc ccggctagcc gccttggcgg ccaacgccca gtttcttccc agcgtgatcg
      781 tcggcgtggc ttgcaggctt gcgcagccct gcttcggagc cacgatcgcg ctccggcgcg
      841 acatgctggt ccgaatcggc gggttcgagc aatttgccga ttcgctggcg gatgattatt
      901 cgatcgggga ggcagtgcgc gcgatgggcg ccgaggtggc gatctcggcg gtttcggtcg
      961 gccatttgtg caaggaccaa accgcccggc agctctgttc ccatgaatta cggtgggggc
     1021 ggacgatccg gtccatcgac ccggtcggct atttcagctc gatcatcacc catccgttcc
     1081 cgctcgcgct gctggcggcg ctgctgggag ccggcggggg ggccgtggcg ctggcggtgc
     1141 ttgccgtgat ttgccgggtc acattattga aatgcgtcga gcggcgcttc agcctggagc
     1201 gacagcccta ttggctggtg ccgctgcggg acctgatttc gtttgtgatc ttcgtctgga
     1261 gcctgttcgg caccagcgtc gactggaacg gcgaaaccta tcaggtaaca tcgcagggga
     1321 tcctggtgcg caggacggcc gtgtgatgcg cacgctattc ctgcaggcgc cctcattcga
     1381 gggcttcgac ggcggcgccg gctcccgcta ccaggcccgc cgcgaaattc gctcgttctg
     1441 gtatccgacc tggctggcgc aacccgcagc cctggtgccc ggcagcaggc tcatcgatgc
     1501 gccgtcacat ggggtttcgc tgaatgaaat cgccgcccag gccgatcgat tcgacctcgc
     1561 cgtgttgcac acctcggcgc catccttcgc cgcggatctc gccgcaatcg aagccctgaa
     1621 acgcggcaat ccgcggctca aggcgggctt gatcggcgcc aaggtcgcgg tcgatccgga
     1681 aggaagcctg aaggcttccg ccgccgtcga tt
//
LOCUS       DASWGM010000441         3940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_167888,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000441 DASWGM010000000
VERSION     DASWGM010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167888"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VGA15_12775"
     CDS             <1..619
                     /locus_tag="VGA15_12775"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9588599.1"
                     /translation="RKDFPAQDLKEFVAVLRQQGGAVKQAHGGIGASSHMACLLFTAE
                     IGAKPTLVAYRGSGPALNDLVGGHVDFMCEQSVSVADQVLAGSVKAYAVSARERLATL
                     PDVPTAKEAGLNYEMSVWAGLFAPKGVPPKVIAKLADALDKALDDTVVRDTLSRLGGS
                     IPAKAERTPAAFDRFVRSEVARWAPILTATQAENASDTSNAGTTR"
     gene            complement(1592..2365)
                     /locus_tag="VGA15_12780"
     CDS             complement(1592..2365)
                     /locus_tag="VGA15_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9588600.1"
                     /translation="MTDYVRREIADGIMTLTISRPDKKNALSNAMYSALSDGLELAEQ
                     DSSVGVVLFQGDGDSFTAGNDLADFSAEANDKSAGESQAARFIGNLARATVPLVAAVQ
                     GNAVGVGTTMLLHCDLVYLADNAKLITPFVNLALVPEASSSWLLPARIGHARAYAMFA
                     LGEPMDASSALACGLANAVVPLSELRTRAHGAAAALTKRPAGSLKQTKALMRETDRIV
                     AQIARESALFRDRLQSGEAREAFAAFAEKRKPDFSKVSG"
     gene            complement(2451..3452)
                     /locus_tag="VGA15_12785"
     CDS             complement(2451..3452)
                     /locus_tag="VGA15_12785"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX9588601.1"
                     /translation="MKTAITEMFGIEHPIIQGGMHYVGFAELAAAVSNAGGLGIITGL
                     TQRTPELLAKEIARCHDLTDKPFGVNLTFLPAFSAPPYPEYIAAIREGGINIVETAGR
                     SPEQYMPALKAAGIKVIHKCTSVRHSLKAEQIGCDAVSVDGFECGGHPGEDDMPNMIL
                     LPRAADELKIPFVASGGMADARSLVAALALGAAGMNMGTRFIATKEAPVHENVKQALV
                     AATELDTRLVMRALRNTERVLRNAGVDRLIEIEREKGRSLKIEDIHDEVAGVYPKVMI
                     DGDMDAGAWSCGMVVGLIKDVPTVKELIDRIMADAESIIRSRLTGFLDDGKAIRRVA"
     gene            complement(3521..>3940)
                     /locus_tag="VGA15_12790"
     CDS             complement(3521..>3940)
                     /locus_tag="VGA15_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9588602.1"
                     /translation="KVWEMACYSGFLAGREAARLMLPRGQGNIFFTGATASLRGGAGY
                     AAFASAKFGLRAVAQATARELGPKNIHVAHLIIDSGVDTEWVRQRRLEALGPGALDNP
                     DLLMPPSSVAESYWQLYQQPRSAWTFELEIRPFGEKW"
BASE COUNT          704 a         1223 c         1239 g          774 t
ORIGIN      
        1 gcggaaggat tttccggcgc aggacctgaa ggaattcgtt gccgtgctcc ggcaacaggg
       61 tggtgccgtg aaacaggcac atggcggcat cggcgcgtcc tcgcacatgg cgtgcctgct
      121 gttcaccgcg gagatcggcg ccaagcccac gctcgttgcc tatcgcgggt ccggtccggc
      181 gttgaacgat ctggtgggag gccacgtcga tttcatgtgc gagcagtcgg tcagcgtggc
      241 ggaccaggtt ctggccggct cggtcaaggc ctacgccgtc tccgcccgcg agcggctggc
      301 gacgctgcct gacgtgccga cagccaagga agcgggcttg aactatgaaa tgagcgtctg
      361 ggctgggcta tttgcgccga aaggtgtgcc gcccaaggtg atcgccaagc ttgcggacgc
      421 cctcgacaag gcgcttgatg acactgtcgt gcgcgacaca ctgtcccggc tcggcggctc
      481 cataccagcc aaggccgagc gcaccccggc cgcattcgat cgttttgttc ggtccgaagt
      541 cgcgcgctgg gcgcccattc tcacggcgac ccaggcggag aatgcgagtg acacgagcaa
      601 cgccggaacg acgcgctgac ctagtctacg gcaacagccc tgcatcggag gggatgcggt
      661 tcttggagag cgaacatcgg gagatgattt ctcctgcctc gagacggcct cgctgtagcg
      721 ggaggattcg aacctccccc aagagtagcc tgttgatttg cccgtcgggc ggtttgttga
      781 caggggtgtc gagtcccttt tgcaaaaata tttcggtttt cacatacccc aaatcacatc
      841 tacaactctt cgcatcccac cccacaagag gggcgtatca tgatcgtcac ggacgcgggg
      901 agggatgcgg tggacgcggc agcgttttgc gcgcgatggg atcgcagggc tggtcgaaag
      961 acttgtgagc gatcaacagc acgctgacga gagacgattg ctgcggacgg caaaatcgtg
     1021 tggtcctgac gccccgacgc tggcgtcaag ttcgcggagt tgcgtcggcc caaccgggct
     1081 tagacaaaac atttccgctg atgacggtgg caaaagagcc cggtcaccgg ggagagcacg
     1141 acataagctg ttaaaaccat tgcgtgcggg aatgccgggt gatcccggtg tacctgtggc
     1201 ttctacccct gtgctttcta cattgcacag ggggctgcgg gtgcatcggg cacccggcat
     1261 tccccacgcc ctcaaggggg cggaagataa atgcacagct cgggcgcttc gcgccgcgag
     1321 atcgcggatg cgcatctgga attggagcag agacgaggaa tgcgtgcccc ggatgctgcg
     1381 cagcgccatc agcgcgttca cgcgcgtctt cgacgcgcta tgtcttgcgg cgtggtgcgc
     1441 tgctgatccg gggcccatct tacactccgc gtcgccgtgg gtcccggctc tgcggtgcat
     1501 cgtgaagaac gctgcaccgc gtccgggaca cgagatgacg gctacaactg aattgtcctt
     1561 ggctgtttga aaattgaatc gggactctgg tctagccgga caccttcgaa aagtccggct
     1621 tacgtttctc ggcgaaggcc gcgaaggctt cccgcgcctc accggattga agccgatccc
     1681 tgaacagggc gctttcgcgg gcgatctggg caacgatccg gtcggtttcg cgcatcaagg
     1741 cctttgtctg tttgagagag ccggcgggcc gtttcgtcaa tgccgcggcg gcaccgtgcg
     1801 cccgggtccg cagctcggac agcggcacga cggcattcgc cagtccgcaa gccagcgcgg
     1861 aagacgcatc catcggctcg ccgagtgcaa acatggcata ggcccgcgca tgcccgatcc
     1921 gggccggcaa cagccagctc gatgaagctt ccggcacaag cgccaggttg acgaacggcg
     1981 ttatcagttt cgcgttgtcg gcaagataga cgagatcgca atgcagcaac atcgtcgtgc
     2041 cgacgccgac cgcgttgcct tgcaccgcgg caacgagtgg cacggttgcc cgcgcgaggt
     2101 taccgataaa gcgagcggcc tggctctcgc cggcgctctt gtcgttcgcc tcggcactga
     2161 aatcggccag atcgtttcca gccgtaaagc tgtcgccgtc gccctgaaac aacacgactc
     2221 cgactgacga atcttgttcc gcaagctcca gtccgtccga aagcgcgctg tacatcgcgt
     2281 tgctcagcgc attcttcttg tccgggcggg aaatagtcaa cgtcatgatg ccatcggcga
     2341 tctcgcggcg aacgtagtcg gtcattgggt tattccctgc gttatcgatg gattgtcacg
     2401 ttccggcggt cgtcgtgacg cgggctttgg cgccggccgg catcggcctt ttacgccacc
     2461 ctccgtatcg ctttcccatc atcgagaaag cccgtcaggc ggctacggat gatactctcg
     2521 gcatccgcca tgatgcggtc gatcaattcc ttcaccgtcg gaacgtcctt gatcaagcct
     2581 acgaccatgc cgcaactcca ggcgccggca tccatgtcgc cgtcgatcat cacctttgga
     2641 tagacgcccg cgacctcgtc atggatgtct tcgatcttga ggctcctgcc cttctcccgt
     2701 tcgatctcga tcagacggtc gacgccggca ttcctgagga cacgctcggt gttccgcagc
     2761 gcgcgcatga ccagcctggt atcgagctcg gtagccgcga ccagcgcctg cttgacgttc
     2821 tcatgcaccg gcgcttcctt ggtcgcgata aagcgcgttc ccatgttcat gccggccgcg
     2881 ccgagcgcaa gtgctgccac caggcttcgc gcgtcggcca tgccgccgga ggcaacgaac
     2941 ggtattttca gttcgtcggc ggcgcgcggc agcaggatca tgttgggcat gtcgtcttcg
     3001 ccgggatgtc cgccgcattc gaagccatcg acgctgaccg catcgcagcc gatctgctcg
     3061 gccttcagcg agtgccgaac cgacgtgcat ttgtggatca ccttgatgcc ggccgccttc
     3121 agcgccggca tatattgctc ggggctgcgt cctgcggttt cgacgatgtt gatcccgcct
     3181 tcccggatgg ccgcgatgta ttccggatag ggcggagcgc tgaacgccgg cagaaaggtc
     3241 aggttgacgc cgaacggctt gtccgtcagg tcgtgacagc gcgcgatttc cttggccagt
     3301 aattcgggcg tcctttgggt caggcccgta ataatgccaa ggccacccgc gttcgagaca
     3361 gcggctgcga gttctgcaaa cccgacataa tgcattccgc cctggatgat cggatgttcg
     3421 atgccgaaca tttcagtgat tgctgttttc aagaatggct cccgtctgtt tgcagcactt
     3481 tggatcaatg gacgatttca agaaggcccg cgggctcgcg ctaccacttc tcgccgaagg
     3541 gtcgtatttc cagctcgaac gtccaggcgc tacgtggctg ctgatagagc tgccagtagg
     3601 attccgcgac cgacgacggc ggcatcagga gatcggggtt atcgagggcc cccggtccca
     3661 gcgcctccag ccgccgctga cgcacccatt cggtgtcgac gccggagtcg atgatgagat
     3721 gggcaacgtg gatgttcttc gggccaagct cgcgcgcggt cgcctgtgcg acggcccgca
     3781 agccaaactt ggcgctggcg aaggccgcat agccggctcc gccgcgcagg ctcgcggtcg
     3841 caccggtgaa gaagatattg ccttggccgc ggggcagcat caggcgcgcg gcttcgcggc
     3901 cggcgagaaa gccggaatag caggccattt cccagacctt
//
LOCUS       DASWGM010000442        10925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1000105,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000442 DASWGM010000000
VERSION     DASWGM010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000105"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VGA15_12795"
     CDS             complement(<1..299)
                     /locus_tag="VGA15_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588603.1"
                     /translation="MIFQRIVVGLLGLVIAGLAAALYIDWKMSVQLRTVKAFVEGYVF
                     AEAVAACERVQSTTPFKWNTGTHTAVIGLNSVKLDKNTISASYTLVADSVYCDYDP"
     gene            complement(330..605)
                     /locus_tag="VGA15_12800"
     CDS             complement(330..605)
                     /locus_tag="VGA15_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEX9588604.1"
                     /translation="MVYTISTGFAIYFVLWWIVLFVTLPFGIRSQHEDGGGVAGTDPG
                     APIASQMGRKLIWTTAISAVVFAAAMLAYNAGYLNVERLSKLMGIPL"
     gene            complement(615..1019)
                     /gene="mce"
                     /locus_tag="VGA15_12805"
     CDS             complement(615..1019)
                     /gene="mce"
                     /locus_tag="VGA15_12805"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX9588605.1"
                     /translation="MLGRLNHVAIAVKDAEKAAKIYGAAFGADISAAVPLPEHGVITV
                     FVTLPNTKIEFIQPLGETSPIAKFLERNADGGIHHICYDVPDIIAARDTLIKEGARVL
                     GDGQPKIGAHGKPVLFLHPKDFSGALVEIEQA"
     gene            complement(1123..2793)
                     /locus_tag="VGA15_12810"
     CDS             complement(1123..2793)
                     /locus_tag="VGA15_12810"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX9588606.1"
                     /translation="MARPDELTFAPLGGVGEIGMNLSIYGLGNRHQRSWLAVDLGVSF
                     GDEEHLPGIDLIMPDIRFLEKERKNLIGLVLTHAHEDHFGAIMDLWPKLKCPIYATKF
                     SAALFEAKCASERNPPKIPVTVVPSGGRIDLGPFNIEFIPVAHSIPESHALAIHTSAG
                     TVLHTGDWKIDPTPSIGVPTDERRLRELGDAGVLALVGDSTNAVRDGRSPSEAEVAKT
                     IIELVKAAKGRVAVTTFASNVARLRAVADAAKVAGREVVVVGRAMERVVQVARETGYL
                     DGVQNFRGADLYGHFPPDKVLALCTGSQGEPRAALSRIANDDHPQVTLNKGDCVIFSS
                     RTIPGNEKAVGGIINGLINQGIEVITDRTHLVHVSGHPRRDELRDMISWVRPQLLIPV
                     HGEALHLFEHAKLARAAGVPKVLICRNGDLVKLGPGEPGVIEELPSGRLYKDGSILED
                     SKSRAVVERRRMAFAGCAFVAIAVTEKGELADDPEVDLVGIPEKNTAGEIIDDIVFDA
                     VVSTVEGLPRARRRDADAMAESVRRAVRATINEQWGKKPLCYVHVLMV"
     gene            complement(2793..3605)
                     /locus_tag="VGA15_12815"
     CDS             complement(2793..3605)
                     /locus_tag="VGA15_12815"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX9588607.1"
                     /translation="MTFSLGPRAVSAGYRLAAFDRIGSTNAEAMSRARDGERGPMWFV
                     TSEQTAGRGRRHRPWIAPRGNLASSILEVIDVSPAVAATLGFAAGLALEAALQKVSVE
                     ASLRAAGSDDMKYSLKWPNDVLAGREKLAGILLEAEAVAGGRLGVVVGIGTNVIAAPE
                     GTPTPATSLAALGVHIGAEELFAVLSDSWAEFRGIWDQGHGFGEIRRLWLERAAGLGQ
                     GVAIHSGGAIVEGTFDTVDEQGCMIVRTSDGKRVPISAGDVYFGSVASVGTA"
     gene            complement(3606..5042)
                     /gene="nuoN"
                     /locus_tag="VGA15_12820"
     CDS             complement(3606..5042)
                     /gene="nuoN"
                     /locus_tag="VGA15_12820"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX9588608.1"
                     /translation="MSFESAGYQLLPVLPELVLAAGAMLLLMVGAYRGPQTTSLVTGL
                     AVCLLVLTGVIELWLPAGKLTTFSGSFIVDDFARFLKILTLIGSAATLILSAEFLADP
                     SRRIFEYSILVLLSSLGMMVLISAGDLIMLYLGLELMSLALYVVAASNRDNAKSTEAG
                     LKYFVLGALSSGMLLYGASLIYGFTGTVSFAGIAAGVKTGSVGIVFGLVFLLAGLCFK
                     VSAVPFHMWTPDVYEGAPTPVTAFFASAPKVAALAVFTRVALTAFPGIMSQWQQIVVF
                     VAIASMVLGSFAAIGQKNIKRLMAYSSIGHMGFALVGLAAGTAEGAQGVLVYISIYVA
                     MTLGSFAVILTMKRNGQHVENISDFAGLSRTNPLLAFFFAMLLFSLAGIPPLAGFFAK
                     WYVFVAAIKAGLFTLAVVGVLASVVGAFYYLSIIKVMYFDEPLGKLDPMRMELRTVLA
                     VAGLFNIFFFVYPGPLVSMATVAAKSLF"
     gene            complement(5143..6654)
                     /locus_tag="VGA15_12825"
     CDS             complement(5143..6654)
                     /locus_tag="VGA15_12825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX9588609.1"
                     /translation="MTTWPILSVVTFLPVFGTVLVYVLARGGDEAANRNARWIALWTT
                     LITFAVSLILVWRFDPAQPDFQFVEKASWLASGITYHMGVDGISLPFVILTTALMPFC
                     IIASWKSVQVRVREYMMAFLILETLMVGTFSALDLVLFYLFFEGGLIPMFLIIGVWGG
                     PRRVYASFKFFLYTLLGSVLMLLAIMALYWNAGTTDIPTLMHTAVPRSLQTWAWLAFF
                     ASFAVKMPMWPVHTWLPDAHVEAPTAGSVILAAILLKMGGYGFLRFSLPMFPIASHEF
                     APLIFSLSTIAIIYTSLVALMQEDIKKLIAYSSVAHMGFVTMGIFAGTTQGVAGGVFQ
                     MVSHGIVSGALFLCVGIVYDRLHTREITAYGGLVNRMPLYALVFMVFTMANVGLPGTS
                     GFVGEFMTLIGTFKVSIPTATFATLGVILSACYALWLYRKVVFGALVKPSLASIKDLT
                     FRESLTLMPLVALTILFGFYPKPVLDMSAASVQQLVNNYSTAVAAVKAAALLQ"
     gene            complement(6654..8723)
                     /gene="nuoL"
                     /locus_tag="VGA15_12830"
     CDS             complement(6654..8723)
                     /gene="nuoL"
                     /locus_tag="VGA15_12830"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX9588610.1"
                     /translation="MIQAIVFLPLIGAILAGLISIVGAHARNPSGDTVEPHDDAHGAD
                     AHASDAHDDHAAGDHGHDDHAVEPPAAGSRAAELITTGLLLVSAALSWVVLVDVGFMH
                     HDARIALFPWINSGDLQVVWSLRVDTLTAVMLVVVNTISSLVHLYSIGYMDEDPYRPR
                     FFSYLSLFTFAMLMLVTADNLVQLFFGWEGVGLMSYLLIGFWYQKPSANAAAIKAFVV
                     NRVGDFGFALGIFAIFMLIGSTDLETIFAGAPGLTGKTIDFFGWNADALTLTCLLLFM
                     GAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSPLFELAPNAQAV
                     VMFFGATTAFFAATVGLVQNDIKRIVAYSTCSQLGYMFVAMGAGAYSVGMFHLFTHAF
                     FKALLFLGSGSVIYAMHHEQDIRNMGGLKDKIPFTYIVMVIGTLALTGFPLTAGYFSK
                     DAIIESAFASHNPFAFYGFLMTVIAAGLTSFYSWRLIFKTFHGEPHDQEHYEAAHESP
                     LWMLIPIGILAAGSILAGFPFKELFAGHGVEEFFRESVKMNPHIIEDMHHIPETIAFL
                     PTVMMALGFVVSYVFYIQRPYLPVELARQHQLLYQFLLNKWYFDELYDLIFVRPAKRF
                     GRFLWKVGDGYIIDGFGPDGVSARVLDVTRNVVRLQTGYLYHYAFAMLIGVAGLITWF
                     MFGVGAQ"
     gene            complement(8732..9040)
                     /gene="nuoK"
                     /locus_tag="VGA15_12835"
     CDS             complement(8732..9040)
                     /gene="nuoK"
                     /locus_tag="VGA15_12835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX9588611.1"
                     /translation="MTIGLGHYLSVGAILFTLGILGIFLNRKNIIIILMSIELILLAV
                     NINLVAFSAFLGDIVGQVFALMVLTVAAAEAAIGLAVLVVYFRNRGSIAVEDVNLMKG
                     "
     gene            complement(9037..9675)
                     /locus_tag="VGA15_12840"
     CDS             complement(9037..9675)
                     /locus_tag="VGA15_12840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX9588612.1"
                     /translation="MILPALFFYLFAGICVASAVMVIVSRNPVHSVLFLILAFVNASG
                     LFVLMGAEFLAMILIVVYVGAVAVLFLFVIMMLDVDFTELREGFSDYLPIGLVIGAIF
                     LAELLLIGGGWVINPNVAKSITAPIPTNVSNTEALGLVLYTKYIHYTEIAGMVLLVAM
                     IGAIVLTLRHKANIKRQDINVQNARTPELAMSLRKVASGQGLQDADAAEWVQ"
     gene            complement(9883..10377)
                     /gene="nuoI"
                     /locus_tag="VGA15_12845"
     CDS             complement(9883..10377)
                     /gene="nuoI"
                     /locus_tag="VGA15_12845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX9588613.1"
                     /translation="MSVNINATARSLLLSEFVSAFFLAMRYFFKPKPTLNYPFEKGPI
                     SPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMV
                     KCIYCGLCQEACPVDAIVEGPNFEFATETREELYYDKAKLLANGDRWEREIAKSIELD
                     APYR"
     gene            complement(10385..>10925)
                     /locus_tag="VGA15_12850"
     CDS             complement(10385..>10925)
                     /locus_tag="VGA15_12850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX9588614.1"
                     /translation="GSLNLSTVVEAQNTRGLASLIGLPWLTFLNWYWLPLFPMFVVFY
                     VSALAETNRPPFDLVEAESELVAGFMVEYGSTPYLLFMLGEYVAITTMCAMAAIMFMG
                     GWLPPIAVAPFTWVPGVIWFTLKVFFMFFLFAMAKAIVPRYRYDQLMRLGWKVFLPLS
                     LAMVVIVAGVLHFAGIAPT"
BASE COUNT         2389 a         3475 c         3249 g         1812 t
ORIGIN      
        1 ggatcgtaat cgcaatagac gctgtcggcc acgagcgtgt agctggcact gatcgtgttc
       61 ttgtcgagct tcaccgagtt caatccgatc accgcggtat gggtcccggt attccatttg
      121 aacggggttg tactctgtac cctttcacag gctgcaaccg cttccgcaaa gacgtagcct
      181 tcaacgaagg ctttcacggt ccggagttgg acagacatct tccagtcgat atagagcgcc
      241 gccgccaagc cggcgatcac cagacccaaa agtccgacaa cgattctttg aaaaatcatt
      301 ccctctccca gcccgcaaaa gtgaatcagt cagagcggaa tccccatcag cttcgacagc
      361 cgctcgacat tcagatagcc cgcattatag gccagcatcg cggcagcgaa aacgaccgcc
      421 gatatcgccg tggtccagat cagcttgcgg cccatctggc ttgcgatcgg tgcgccggga
      481 tcagtgcccg ccacgccgcc gccgtcctcg tgctggctgc ggatcccgaa cggcagcgtc
      541 acgaacagca cgatccacca cagcacgaag tagatcgcga acccggttga gatggtgtag
      601 accatcgcta cgcctcaggc ctgttcgatt tcgacgagcg cgccggaaaa atccttcggg
      661 tgcagaaaca gcaccggctt gccatgggcg ccgatcttgg gctgcccgtc gccgagcacc
      721 cgcgcgccct ccttgattag ggtgtcgcgc gccgcgatga tgtcgggcac gtcgtaacag
      781 atgtggtgaa tgccgccgtc ggcgttgcgt tcgagaaatt tggcgatcgg cgaggtttcg
      841 ccgagcggct ggatgaattc gatcttggtg ttgggcagcg ttacgaacac ggtaatcacg
      901 ccgtgctccg gcaacggcac ggccgccgaa atatcggcgc cgaacgccgc gccatagatt
      961 ttagcggcct tctcggcgtc cttgaccgcg atcgccacat gattgagccg gcccagcatg
     1021 acactctctc cgccttggct tgcgcgtgtt gtaaccgcaa aaccggtatc cacccgtcgg
     1081 gtcggagccc gagggtatgc ttttgcggaa cacgcgccaa acttacacca tcagaacgtg
     1141 aacgtaacac aacggcttct tgccccactg ctcattgatc gtggcacgca ccgcccggcg
     1201 caccgactcc gccatcgcgt cggcatcgcg ccggcgggcc cgcggcaggc cttccacggt
     1261 cgacaccacg gcgtcgaaca cgatgtcgtc gatgatttct ccggcggtat tcttctcggg
     1321 tattccgacc agatcaacct cggggtcgtc ggccagttcg cccttttcgg tcaccgcaat
     1381 cgccacgaaa gcgcagccgg cgaaagccat gcggcggcgc tcgaccacgg cacgcgattt
     1441 tgaatcctcc agaatcgagc cgtccttgta gagccgcccc gacggcagct cctcgataac
     1501 accaggctcg cccggaccta atttgaccag atcgccgttg cggcagatca gaaccttcgg
     1561 cacgccagcg gcccgcgcaa gttttgcatg ttcaaacagg tgcagcgcct cgccgtgcac
     1621 ggggatcagc agttgcggac gaacccatga aatcatgtcg cggagttcgt cgcggcgcgg
     1681 atggccggag acatgcacga gatgggtgcg gtcggtaatc acctctatgc cctggttgat
     1741 caggccattg atgatgccgc ccaccgcctt ctcgttgccg gggatggtgc gcgaggaaaa
     1801 gatcacgcaa tcgcccttgt tgagcgtcac ctgcggatga tcgtcattgg caatgcgcga
     1861 cagcgccgca cgcggctcgc cctggcttcc cgtgcacagc gccagcacct tgtcgggggg
     1921 aaaatggccg tagagatcgg cgccgcggaa gttctgcacg ccgtcgagat agccggtttc
     1981 acgcgccacc tgtactacgc gctccatggc gcggccgacc accacgacct cacgcccggc
     2041 caccttcgcc gcgtccgcga ccgcccgcag ccgcgcgacg ttggaagcaa aggtggtgac
     2101 ggcgacgcgc cctttggccg ccttcaccag ctcgatgatg gtcttggcga cttcggcttc
     2161 cgacggcgac cgcccgtccc gcacggcgtt ggtggaatcg ccgaccagcg ccagcacgcc
     2221 ggcgtcgccg agctcgcgca acctgcgctc gtcggtcggc acgccgatgc tcggcgtcgg
     2281 atcgatcttc cagtcgccgg tatgcagcac ggtgccggcc gaggtgtgga tcgccagcgc
     2341 atgggattcc ggaatcgaat gcgcgaccgg aatgaactcg atattgaacg gcccgagatc
     2401 gatccggccg cccgacggca ctaccgtgac cggaattttc ggcggattgc gctcggaagc
     2461 gcatttggcc tcgaacaatg cagcgctgaa tttggtcgca tagatcgggc atttcagttt
     2521 gggccacaga tccatgatgg cgccgaagtg atcttcgtgc gcgtgggtca gaaccaggcc
     2581 gatcagattc ttccgttcct tttcgagaaa acggatatcg ggcatgatca gatcgatgcc
     2641 gggcagatgc tcctcgtcgc caaaggacac gccgaggtcg accgcgagcc aggagcgctg
     2701 atggcggttg ccgaggccgt agatcgacag gttcatgccg atctcgccga cgccgccgag
     2761 cggcgcaaag gtcagttcat ccggtcgcgc catcaggctg tccctaccga cgccaccgag
     2821 ccgaaataga catcgccggc cgatatcggc acccgcttgc cgtccgacgt ccggacgatc
     2881 atgcagccct gctcatcgac cgtatcgaat gtcccttcca cgatggcgcc accggagtgg
     2941 atcgcgaccc cctgtccaag tccggccgcg cgttcgagcc agagccgccg gatctcgcca
     3001 aagccgtggc cctggtccca gataccgcga aattcggccc acgagtccga cagcaccgcg
     3061 aacaactcct ccgcgccgat atggacgccg agtgcggcga gtgaggtcgc cggcgttggc
     3121 gtgccctcgg gtgcggcgat gacgttggtg ccgatgccga ccacgacgcc caggcgaccg
     3181 ccggctacgg cctcggcctc cagcaggatg ccggcgagtt tttctcgtcc cgccaggaca
     3241 tcgttgggcc atttcagcga atacttcata tcgtccgatc ccgccgccct cagcgacgct
     3301 tcgacgctta ctttttgcag cgccgcctcg agcgcaagcc ctgcggcaaa gcccagcgtc
     3361 gccgcgaccg cgggcgaaac gtcgatcact tcgaggatgc tgctggctag attgccacgc
     3421 ggcgcaatcc acgggcgatg ccgccgtcca cgtcccgccg tctgctccga cgtcacaaac
     3481 cacatcggtc cacgctcgcc atcgcgcgcc ctcgacatgg cttccgcgtt ggtcgagccg
     3541 atccgatcga aggcagcgag ccggtaaccc gccgatacgg cccgaggccc cagcgaaaat
     3601 gtcatctaga acagcgattt cgccgcgacc gttgccatgc tgaccagcgg ccccggatag
     3661 acgaagaaaa agatattgaa cagacccgcg accgccagca cggtgcgcag ttccatccgc
     3721 atcgggtcga gcttgccgag cggctcatcg aaatacatca ccttgatgat gctgagatag
     3781 taaaacgcgc cgaccacgct ggcgaggacg ccgaccacgg ccagcgtgaa cagtccggcc
     3841 ttgatggcag cgacgaacac gtaccatttg gcgaagaagc ctgccaaggg cggaatgccc
     3901 gcaagtgaaa acagcagcat cgcgaagaag aatgccagca acggattggt gcgcgaaagc
     3961 cctgcgaaat cgctgatatt ctcgacatgc tgaccgttgc gcttcatggt caggatgaca
     4021 gcgaaggagc ctagcgtcat cgccacatag atcgagatat agaccaatac gccctgcgcg
     4081 ccctctgccg ttccggcggc gagccccacc agcgcgaagc ccatatggcc gatcgaggaa
     4141 taggccatca gtcgcttgat gttcttctgg ccgatggcgg cgaacgatcc cagcaccatc
     4201 gaggcgatcg cgacgaacac cacgatctgt tgccactgcg acatgatgcc cggaaaggcc
     4261 gtaagcgcta cacgcgtgaa taccgcaagt gctgcgacct tcggcgcgga agcaaagaat
     4321 gcggtgaccg gggtcggtgc gccctcatag acgtcgggcg tccacatgtg gaacggcacc
     4381 gccgaaacct tgaagcaaag ccctgcgagc agaaacacca gaccgaacac gatgccgacg
     4441 ctgccggtct tcacccccgc ggcgataccc gcaaagctca cggtgccggt aaagccgtag
     4501 atcagcgagg cgccatacag cagcatgccc gatgacagcg cgcccagcac gaaatacttc
     4561 aggccggctt cggtggattt cgcgttgtcg cggttgctcg ccgcgaccac gtagagcgcg
     4621 aggctcatca gttcgaggcc gagatagagc atgatcagat cgccggccga gatcagcacc
     4681 atcatgccca gcgacgagag cagcaccaaa atggaatatt cgaaaatacg acgggacgga
     4741 tcggccagaa attccgccga gaggatcagc gttgccgccg aaccgatcag cgtcaatatc
     4801 ttcaggaacc gggcaaaatc atcgacgatg aagctgccgc tgaacgtcgt cagctttccc
     4861 gccggcaacc aaagctcgat cacgcccgtc agcaccagca ggcaaaccgc aaggccggtg
     4921 acaaggctcg tcgtctgtgg cccgcgatag gctcctacca tcagcagcag catggcgccg
     4981 gccgccagca ccagctccgg cagcacgggc agcaactgat aacctgcact ctcaaagctc
     5041 atggcgcgtt gtcctgaccc ggagcctcct cgattctgat cgaatcagaa ccgaagaaac
     5101 tccatcctgt ttgtttgacg cgttttcttc acgcgaacct gttcactgca gcagcgcggc
     5161 tgctttcacg gcagccactg cggtgctgta gttgttgacg agttgctgca ccgacgccgc
     5221 cgacatatcg agcaccggct tcggatagaa gccgaacaga atggtgagag ccaccaacgg
     5281 catcagtgtc aggctctcgc ggaatgtgag atccttgatg ctcgccagcg acggcttcac
     5341 caaggcgccg aacaccactt tgcgatagag ccacagcgca tagcatgccg acaggatcac
     5401 gcccagcgtg gcaaaggtcg cggtcgggat cgagaccttg aaggtgccga tcagcgtcat
     5461 gaattcaccg acgaaacccg aggtgccggg cagaccgaca ttggccatgg tgaagaccat
     5521 gaacaccagc gcgtagagcg gcatgcgatt gacgaggccg ccataggccg tgatctcgcg
     5581 ggtgtgcagg cggtcgtaga cgatgcccac acaaaggaac agcgcgcccg agacgatgcc
     5641 gtgcgacacc atctggaaca cgccgcccgc cacaccttgc gtggtgccgg cgaaaatacc
     5701 catggtgacg aagcccatat gcgccacgga ggaataggca atcagcttct tgatgtcttc
     5761 ctgcatcaag gccaccagcg aggtgtagat gatggcaatg gtcgagagcg agaagatcag
     5821 cggcgcgaat tcatgcgacg cgatcggaaa catcggcagc gagaaccgca gaaaaccgta
     5881 gccgcccatc ttcagcagga tcgcggccag gattaccgac cccgcggtcg gcgcctcgac
     5941 atgcgcatcc ggcagccagg tatgcaccgg ccacatcggc atcttcaccg cgaaggacgc
     6001 gaaaaacgcc agccacgccc aggtctgcaa ggaccgtggc accgcagtgt gcatcaaggt
     6061 cggaatgtcg gtggtgccgg cgttccagta cagcgccatg atggcgagca gcatcagcac
     6121 cgagccgagc agcgtgtaga ggaagaactt gaaggaggca tagacccggc gcggcccgcc
     6181 ccacaccccg atgatcagga acatcgggat caggccgccc tcgaagaaca gatagaacag
     6241 cacgaggtcg agcgccgaaa aggtgccgac catcagcgtt tccaggatca ggaacgccat
     6301 catgtattcg cgcacccgta cctgcaccga tttccagctc gcgatgatgc agaacggcat
     6361 caatgcggtg gtcaggatca cgaatggcag tgaaataccg tcgacgccca tgtgataggt
     6421 gatgccgctg gcgagccacg acgccttctc gacgaactgg aagtccggct gcgccggatc
     6481 gaagcgccag accaggatca gcgacacggc aaaggtgatc agcgtggtcc acagcgcgat
     6541 ccagcgtgca ttacgattgg ccgcttcgtc gccgcctcgc gcaagcacat acacaaggac
     6601 cgtaccgaac accggcagga aggtgacgac ggagaggatg ggccaggttg tcatcactgg
     6661 gctcccacgc cgaacatgaa ccaggtgatc aagccggcaa cgccgatcag catcgcgaag
     6721 gcgtagtggt agagataacc ggtctgcagc cgcaccacgt tgcgagtgac gtcaagcacc
     6781 cgtgccgaca ccccatcggg gccgaagccg tcgatgatgt agccgtcgcc gaccttccac
     6841 aggaaccgtc cgaaccgctt cgccgggcgc acgaagatca gatcgtagag ctcgtcgaag
     6901 taccatttgt tgagcaggaa ctggtagagc aattggtgct ggcgggcgag ttccacgggc
     6961 aaatagggcc gctggatata gaaaacgtag gacaccacaa aacccaacgc catcatgacc
     7021 gtcggcagga acgcaatcgt ctcggggatg tggtgcatgt cctcgatgat gtgcgggttc
     7081 atcttcaccg actcgcggaa gaattcttcc acgccatgac ccgcgaacag ttccttgaac
     7141 gggaagccag cgaggatcga tccggcggcg agaatgccga tcggaatgag catccacagc
     7201 gggctttcgt gcgcggcctc gtaatgttcc tggtcgtgcg gctcgccgtg aaaggtcttg
     7261 aagatcaggc gccacgaata gaacgaggtc agtccggccg cgatcaccgt catcaggaag
     7321 ccgtagaatg cgaacggatt atgcgaggcg aacgcggatt cgataatcgc atccttggag
     7381 aaatagccgg cggtgagcgg aaagccggtc agcgccagcg tgccgatcac catcacgatg
     7441 taggtgaagg ggatcttgtc cttcaagccc cccatgttgc ggatgtcctg ctcgtgatgc
     7501 atcgcataga tcaccgagcc cgagcccaag aacaatagcg ccttgaagaa ggcgtgggtg
     7561 aacagatgga acatgccgac ggagtaagcc cccgccccca tcgccacgaa catgtagccg
     7621 agctgcgaac aggtcgaata cgccacgatg cgcttgatgt cgttctgcac gaggccgacg
     7681 gtcgccgcga agaacgcggt ggtggcgcca aagaacatca cgacggcctg cgcgttcggc
     7741 gccagttcaa acagcggcga cagccgcgcc accatgaaga caccggcggt gaccatggtc
     7801 gcggcgtgga tcaatgcgga gaccggcgtc ggaccttcca tggcgtccgg caaccaggtg
     7861 tgcagcagga actgggcgga cttgcccatc gcgcccatga acaatagcag gcaagtcagc
     7921 gtcagcgcat cggcattcca gccgaagaag tcgatggtct tcccggtcag ccccggcgcg
     7981 cccgcgaaga tcgtttcaag gtcggtcgac ccgatcagca tgaagatcgc gaaaataccg
     8041 agtgcaaagc cgaaatcgcc gacccgattg accacgaagg ccttgatcgc ggcggcattg
     8101 gccgacggct tctggtacca gaacccgatc agcagatagc tcatgaggcc gacgccttcc
     8161 cagccgaaga acagctgcac cagattatcg gcggtcacca gcatcagcat ggcgaaggtg
     8221 aacagcgaca gatagctgaa gaaccgcggc cggtacggat cctcgtccat gtagccgatc
     8281 gaatagagat gcacgagcga cgagatggta ttgaccacca ccagcatcac cgcggtcagt
     8341 gtatcgaccc gcagcgacca caccacctgc agatcgcccg aattgatcca gggaaacagc
     8401 gctatccgcg cgtcgtgatg catgaagccg acatcgacca gcaccaccca ggataacgcc
     8461 gccgaaacca gcaacaatcc ggtcgtgatc agttcggccg cgcgcgaacc cgccgccggc
     8521 ggctcgaccg cgtgatcgtc gtggccgtga tcgccggcgg catgatcgtc atgggcatcc
     8581 gacgcatggg catcggcgcc gtgcgcgtcg tcatgaggct ccacggtgtc gccgctcgga
     8641 ttgcgtgcat gcgcaccgac gatagagatc aggcccgcga gtattgcgcc gatcagcggc
     8701 agaaagacga ttgcctggat catttggcgc gttaaccctt catcagattg acgtcttcaa
     8761 ccgcgatcga gccgcggttg cggaagtaca ccaccagcac cgccagcccg atcgcagctt
     8821 ccgccgcggc caccgtcagc accatcagcg caaacacttg gccgacgatg tcgcccagga
     8881 aggccgagaa cgccaccagg ttgatattca ccgcgagcag gatcagttcg atcgacatca
     8941 ggatgatgat gatgttctta cggttgagga agatgccgag aatgccgagc gtgaacagga
     9001 tcgcgccgac cgacagataa tgtccgagac cgatcgtcat tgcacccact ccgcagcgtc
     9061 cgcgtcctgc aggccctgcc cggaggccac cttgcgcagg ctcatggcaa gttcgggcgt
     9121 ccgcgcgttc tgcacattga tgtcctggcg cttgatgttg gccttgtggc gcagcgtcag
     9181 cacgatggcg ccgatcatcg cgaccagaag caccatgccg gcgatctcgg tgtaatggat
     9241 atacttcgta tagagcacga ggccgagcgc ttcggtattg ctgacattcg ttgggatcgg
     9301 cgcggtgatc gatttggcga cattggggtt gatgacccag ccgcccccga tcagcagcag
     9361 ttcggccagg aatatcgcgc cgatcacaag accgatcggc aaatagtcgc tgaagccttc
     9421 ccgcagttcg gtgaaatcga cgtcgagcat catgatcacg aacaggaaca gcaccgcgac
     9481 cgcgccgaca taaaccacga ttaggatcat cgccagaaat tcggcgccca tcagcacgaa
     9541 caggccggac gcgttgacga acgccaggat caagaacagc acggagtgca cgggattgcg
     9601 cgacacaatc accatcaccg ccgaggccac gcaaatgccg gcgaacagat agaagaacag
     9661 cgcgggaagg atcatgcgaa cgcgcctccg ctataagcca cgctgccatc cgcgaacgtt
     9721 accgtgttcc ctcccccctt gtgggggagg gttagggagg ggggtaagcc acaagcactg
     9781 agatcggggc ttacccccct ctccagctct cccccacaag gggggagaga gccgatcgtg
     9841 ctcgtggcac gtccgcgcat ctcattcgcc aaattcatca cctcaccggt acggcgcgtc
     9901 gagttcgatt gatttcgcaa tctcgcgctc ccagcgatcg ccgttggcga gaagtttcgc
     9961 cttgtcataa tagagttcct cgcgggtctc ggtcgcgaat tcgaaattcg gtccctcgac
    10021 gatggcatcg accggacagg cctcctggca caggccgcaa tagatgcact tcaccatatc
    10081 gatgtcgtag cgcacggtgc ggcgggtgcc gtcgttgcgg cgcgggccgg cttcgatggt
    10141 gatcgcctgc gccggacaga tcgcctcgca cagtttgcag gcgatgcagc gttcctcgcc
    10201 attgggatag cggcgcagcg catgctcacc gcggaaccgc ggcgagatcg gccctttctc
    10261 gaacggataa ttcagcgtcg gcttcggctt gaagaaatag cgcatggcga gaaagaacgc
    10321 cgacacgaat tcggacagca gcagcgagcg ggccgtggca ttgatgttga cactcatagc
    10381 gacctcatgt cggcgcgatc ccggcgaaat gcagcacgcc ggcgacgatc accaccatcg
    10441 ccaacgacaa cggcaggaac accttccagc cgagccgcat caactggtcg tagcggtagc
    10501 gcggcacgat cgccttcgcc atcgcaaaca ggaagaacat gaaaaatacc ttgagcgtga
    10561 accagatcac cccgggcacc caggtgaacg gcgccaccgc gatcggcggc agccagccgc
    10621 ccatgaacat tatcgctgcc atcgcgcaca tcgtggtgat cgcgacatat tcgccgagca
    10681 tgaacaaaag atacggcgtc gagccgtatt cgaccatgaa gcccgcgacc agttcggact
    10741 cggcttccac cagatcgaac ggcggacgat tggtttccgc cagcgccgag acatagaaca
    10801 ccacgaacat cgggaacagc ggcagccagt accagttcag gaacgtcagc cacggcaatc
    10861 cgatcaggct tgcaagacca cgcgtgttct gcgcctcgac cacggtcgag aggttcagcg
    10921 atccc
//
LOCUS       DASWGM010000443         6008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1986220,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000443 DASWGM010000000
VERSION     DASWGM010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986220"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..889)
                     /locus_tag="VGA15_12855"
     CDS             complement(68..889)
                     /locus_tag="VGA15_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9588615.1"
                     /translation="MRSVKSLIAAGVASLLSSMAFAADMPSIMSAPMYAPAPVEDFGG
                     WYLRGDIGFSNQSVKKLDWFSYPTLLSLNQNTGFDTAGIFGIGAGYQFNNWLRADVTG
                     QYRGKSNFHGTDVFVFSNAGVPTAGADTYTASKSEWLVLANAYVDLGTWWYVTPFIGA
                     GVGFSRNTISSFVDTNTPTGGVAIAPTASKWNLAWALHTGLAYRVNPGFTVELGYSYV
                     NLGDATTGIVADFTGFANNHTMGFKDITSHDLTLGVRWNLDSPTVYAPPPVVRKG"
     gene            complement(1126..2385)
                     /locus_tag="VGA15_12860"
     CDS             complement(1126..2385)
                     /locus_tag="VGA15_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9588616.1"
                     /translation="MNKSVVISEETLVAPVLVPDAASDSVNKPSAAGPAYIVLAGISL
                     SHFLNDTMQSLIASIYPILKDNYALDFAQIGLITLAFQFTASLLQPVVGYFTDRKAQP
                     FSLAIGMGSTFFGLLLLSIARHYPVILVAAALVGLGSAVFHPESARIARLASGGRYGF
                     AQSVFQLGGSFGTSMGPVLAALIVVPFGQPSIAWFSSIAFVAIVILWRIGVWYKPQIA
                     AKKFAPVEPHPDAPDSRRVRIALAVLVALLFSKQLYVASLSSYYIFYLIDKFGVSTQG
                     AQFYLFIFLAANAVGAFFGGPMGDRFGRKYVIWFSILGALPFTLALPYAGLFGSAVLT
                     VFIGFIISSATSSIIVFAQELMPHRFGMISGVFFGVAFGIGGLGAAVLGKVADHTGIA
                     FVYQVCAFLPAIGLLAVFLPKMPKARR"
     gene            complement(2513..3859)
                     /gene="glmM"
                     /locus_tag="VGA15_12865"
     CDS             complement(2513..3859)
                     /gene="glmM"
                     /locus_tag="VGA15_12865"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX9588617.1"
                     /translation="MSRKYFGTDGIRGRANGLITPELALKVGQAAGLVFQRGEHRHRV
                     VIGKDTRLSGYMIEYAMVAGFTSVGMDVLLLGPMPTPAVAMLTKSMRADLGVMISASH
                     NLFEDNGIKLFGPQGFKLSDDVEKQIEQLLDESLDKRLAKSASLGRARRIDGVHDRYI
                     EFAKRTLPRDLSLDGMRVVVDCAHGAAYRVVPEALWELGADVIAIGVDPDGFNINKEC
                     GSTSPEALSRKVREMRADIGIALDGDADRVILVDERGHLVDGDQLLAVIAQSWKEDGR
                     LTKPGIVATVMSNLGLERFLEGLGLEMVRTPVGDRYVLEQMLSRGYNLGGESSGHIIL
                     SDYATTGDGFVAALQVLAVVQKLRRPVSEVCHRFDPLPQILKNVRYRSGKPLDDADVK
                     SAIDAGEKRLNGHGRLLVRSSGTEPVIRVMGEGDDRNLVEEVVDHIVSAIGHAAAA"
     gene            complement(4075..4242)
                     /locus_tag="VGA15_12870"
     CDS             complement(4075..4242)
                     /locus_tag="VGA15_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588618.1"
                     /translation="MTGPRSDPKDVKGAEKRRLDQALEEGLEETFPASDPVNVTQPPP
                     SKGDHYVKRKD"
     gene            4350..5486
                     /locus_tag="VGA15_12875"
     CDS             4350..5486
                     /locus_tag="VGA15_12875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX9588619.1"
                     /translation="MKHITCIEDLRQVHMRKVPKAFFDYADRGSYAEETLRANRDDLQ
                     QIKFRQRILVDVSKRDLSTTILGEPASLPLILAPIGLCGMQFGDGEIHACRAAQAAGI
                     PFTLSTMSICSIEDVAANVDKPFWFQLYVMKDRGFIKALIERAIAAKCSALVLTVDLQ
                     VIGQRHADIKNGMTVPPEWSVSKFLDFASKPGWVSGVLRGKHRTFGNLMGHLKGTDDI
                     TALGTWISTQFDTTLNWKDVEWIRSIWPGKLILKGILDVEDAEEAAKTGAQALVVSNH
                     GGRQLDGAPSSIEVLPEIVDVVGSQMEVMFDGGIRSGQDVMRALALGAKSCMIGRAYI
                     HGLGAFGGPGVAKAIDIIANELSVTMGLCGVNTIAEIDDHVLAV"
     gene            complement(5732..>6008)
                     /locus_tag="VGA15_12880"
     CDS             complement(5732..>6008)
                     /locus_tag="VGA15_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588620.1"
                     /translation="WDLRPNSTMTNATVAETVKGTDGQNILVKYKDGEKKVVVPPDTP
                     IVTFVAGDKSELKPGAKIIIFGAVKKDDGTLEANRVNVGRDGITPPM"
BASE COUNT         1259 a         1850 c         1783 g         1116 t
ORIGIN      
        1 gacgcaaaac aaaacggcgc ggaaaaatcc cgcgccgtta gcaatcgtta agagatgaga
       61 gctgcgatta acctttgcgt accacaggcg gcggcgcata cacggtcggg ctgtcgagat
      121 tccagcgtac gccgagcgtc aggtcgtgcg aggtgatgtc tttgaatccc atcgtatggt
      181 tattcgcgaa cccggtgaaa tcggcgacga ttccagtcgt ggcgtcgcca agattcacgt
      241 agctatagcc cagctccact gtgaagcccg gattgaccct gtaagccaga ccggtatgca
      301 gagcccatgc gaggttccac tttgaggctg taggtgcgat agcgacaccg ccggtcggcg
      361 tatttgtatc gacaaaactg gagatcgtgt tgcgcgaaaa gcctacgcca gcgccgatga
      421 acggcgtcac gtaccaccaa gtaccgagat ccacataggc gttggccaga accagccatt
      481 cggacttgct agcggtgtaa gtgtcagcgc cggccgtagg cacgcccgcg ttgctgaaga
      541 caaaaacatc ggttccgtga aaattggatt taccgcgata ctggccggtg acgtcggcgc
      601 ggagccaatt gttgaactgg taaccggcgc caatgccgaa gatacccgca gtgtcaaagc
      661 ccgtattttg attcagcgac agcagtgtag gataggaaaa ccaatccagc ttcttgacgc
      721 tttgattgct gaatccgata tcgccgcgca gataccatcc gccgaaatcc tccaccggcg
      781 ccggcgcata catcggcgcc gacatgatgg aaggcatgtc cgcggcgaac gccatcgacg
      841 acaacagtga tgccactccg gcggcaatga gagacttcac gctacgcatt ggcttcgtcc
      901 ttttggccgg tgaggcagac aacgcgaggc cccacatcta aaaactcacg gcaggacgat
      961 ggcaccaaat gtttaagcgc cacttaaccc taattattaa ggttgataat ccgttgcact
     1021 tgatccgcaa gctgtgagcg gcagcccgga aattggcgga gtctttacga tcgctaacaa
     1081 tgtattgatg aaaatccgct ggcgtctgat ctgaagcgta atcagtcagc gccgggcttt
     1141 cggcatcttc ggcaggaaca ccgcgagcaa cccgatcgcc ggcaggaacg cgcagacctg
     1201 atagacgaag gcgatgccgg tgtggtcggc gaccttgcca agcaccgcgg cgccaagccc
     1261 gccgatcccg aatgcgacgc cgaagaacac cccggagatc attccgaaac gatgcggcat
     1321 caattcctgc gcgaacacga tgatcgatga tgtcgccgac gagatgatga agccaatgaa
     1381 taccgtcagc accgcgctgc cgaacaggcc ggcgtagggc agcgccagcg tgaacggcaa
     1441 cgctccgagg atcgaaaacc agatgacgta tttgcggccg aaccgatcgc ccatcggtcc
     1501 accgaaaaac gctccgaccg cgttggcggc gagaaaaatg aagagataga actgcgcgcc
     1561 ctgggtcgag accccgaact tgtcgatcag atagaaaatg taatagctcg acaggctcgc
     1621 cacatagagc tgcttggaga acagcagcgc gaccagcacg gcaagcgcta ttctgacgcg
     1681 gcgcgaatcc ggcgcgtccg gatgcggttc gacgggtgcg aactttttcg ctgcgatctg
     1741 aggtttgtac caaacgccga ttcgccagag aatgacgatc gcgacaaatg cgatcgatga
     1801 aaaccaggcg atgctcggct gaccgaacgg cacaacgatc aaggccgcca gcaccgggcc
     1861 catcgaggtg ccgaaactgc cgccaagctg aaataccgat tgcgcgaagc cgtagcgacc
     1921 gcccgatgcg agccgcgcga tccgcgccga ttccggatgg aacacggccg agcccagccc
     1981 gaccagcgcc gctgcgacca ggatgacagg ataatgccga gcgatactga gcagcagcag
     2041 gccgaagaag gtagatccca tgccgatggc gagcgaaaac ggctgcgcct tcctgtcggt
     2101 gaaatatccg accaccggct gcagcaacga tgcggtgaac tggaacgcca gcgtgatcaa
     2161 tccgatctgc gcgaagtcca gcgcgtaatt gtctttcagg atcggataga tcgacgcgat
     2221 cagcgactgc atggtgtcgt tgaggaaatg cgagaggctg attcccgcca gcacgatgta
     2281 ggccggtccc gccgcgctcg gcttgttgac ggaatcagac gccgcgtccg gcaccagcac
     2341 cggcgcaacc agtgtttctt cggagatgac cactgactta ttcaagggac gacttgttca
     2401 aaagaatgat tcccggcacc cggcgcgaca gccatttcct acgatgccgg cggcgcgccg
     2461 accagcacca ttagataatg gctgcgatgc gattggacgc ggttaaccgc gctcaggccg
     2521 ccgcggcatg gccgatcgcg gagacgatat gatcgaccac ttcctcgacc aggttgcggt
     2581 catcgccctc gcccatcacg cggatcaccg gctcggtgcc ggacgagcgg accagcaacc
     2641 ggccatgacc attgagccgt ttttcgccgg cgtcgatggc cgatttgaca tcggcgtcgt
     2701 cgagcggctt gccgctgcga tagcgcacgt tcttcagaat ctgcggcaag gggtcgaagc
     2761 gatgacagac ctccgacacc gggcggcgaa gtttttgcac caccgcgagc acctgaagtg
     2821 cggcgacaaa gccgtcacct gtggtggcgt agtccgacag aatgatgtga ccggaggact
     2881 cgccgccgag attgtaaccg cggctcagca tctgctcgag cacatagcga tcgccgaccg
     2941 gcgtgcgcac catctcaagc cccaggcctt cgaggaatcg ctccagtccg agattcgaca
     3001 tcacggtcgc gacaatgccc ggcttggtga gtcggccgtc ttccttccag ctctgcgcga
     3061 tcaccgccag taactggtcg ccgtcgacca ggtgtccgcg ctcatcgacc agaatgaccc
     3121 ggtcggcgtc gccatcgagc gcgatgccga tgtcggcgcg catttcgcgc accttgcggc
     3181 tcagcgcttc cggcgaggtg gatccgcatt ccttgttgat attgaatccg tcggggtcga
     3241 cgccgatggc gatcacatcg gcacccagct cccacagcgc ttccggcacc accctatagg
     3301 ccgcgccgtg ggcgcaatcc accaccacgc gcatgccatc aaggctgaga tcgcgcggca
     3361 atgttcgttt ggcgaattcg atgtagcgat cgtggacgcc gtcgatgcgg cgggcgcgcc
     3421 cgagactggc gctcttcgcc aggcgcttgt cgagcgattc gtccagcaac tgctcgatct
     3481 gcttttcgac gtcgtcggat agcttgaaac cttgcgggcc aaacagcttg atgccgttat
     3541 cctcgaacag attgtgcgac gccgaaatca tcacgccgag atcggcgcgc atcgacttgg
     3601 tcagcatcgc caccgccggc gtcggcatcg ggccgagcag cagcacatcc atgccgaccg
     3661 aggtaaagcc ggccaccatc gcgtattcga tcatgtagcc ggaaagccgc gtgtccttgc
     3721 cgatcacgac gcgatggcga tgctcgccgc gctggaacac caatccagcc gcctgcccga
     3781 ccttcagcgc cagttccggc gtgatcaggc cgttggcccg gccccgaatc ccatccgtac
     3841 cgaaatattt gcggctcata ttccccccag cggctttcaa ggattttcaa accaccccgc
     3901 gagaagcctc gaacgccggt ctatcatagc gtgtacgcct tacaatgcgc tcgacgctag
     3961 aatggcttca aaaaatatga tgaatcatta ccgccggaca atccggtcct tggcccctaa
     4021 cacgttgtta acgcacgcat tactgccaga tccgaaccct ggcacaatgc agcgtcagtc
     4081 cttgcgcttg acgtaatggt cgcctttcga gggcggcggc tgggtgacat tgaccgggtc
     4141 cgaagccgga aaggtctcct ccagaccctc ctcgagtgcc tgatcgagcc ggcgcttctc
     4201 agcacctttg acgtccttgg ggtcggagcg tggtccggtc atggatgccc tcctgttacg
     4261 agccggttcc gcgacacgat tttgcggctg gcccaaccat gctagatgac tgcgccagcg
     4321 aagaattaca agaagggtcg ggaacgtcca tgaaacacat tacctgcatc gaggatctgc
     4381 gccaggtcca tatgcgcaaa gtgccgaagg cgtttttcga ctacgccgat cgcggctcct
     4441 atgccgagga aacgctgcgc gccaatcgcg acgacctgca gcaaatcaag tttcgccagc
     4501 gtatcctggt ggatgtatcg aaacgcgatc tttcgaccac catcctcggc gagcccgcat
     4561 cgctgccgct gatcctggcg ccgatcgggt tgtgcggcat gcaattcggc gacggagaga
     4621 tccatgcctg ccgcgccgcg caggccgccg gcattccctt cacactctca acgatgtcga
     4681 tctgctcgat cgaggatgtc gcggcaaatg tcgacaagcc gttctggttt cagctctacg
     4741 tcatgaagga ccgcggcttc atcaaggcgc tgatcgagcg cgcgatcgcg gcgaaatgca
     4801 gcgcgctggt cttgaccgtc gacctgcagg tgatcggaca gcgtcatgcc gacatcaaga
     4861 atggcatgac ggtgccgccg gaatggagcg tgtcgaaatt cctggatttc gccagcaagc
     4921 ctggatgggt atcgggcgtg ctgcgcggca agcatcgcac cttcggcaat ctgatgggcc
     4981 acctcaaggg caccgacgac atcaccgcgc tggggacctg gatctcgacg caattcgaca
     5041 ccacgctgaa ctggaaagac gtcgagtgga tccgcagcat ctggcccggc aagctgatcc
     5101 tcaagggtat tctcgacgtc gaggacgccg aggaagcggc gaagaccggc gcccaggcgc
     5161 tggtggtctc caatcacggc ggccggcagc tcgacggcgc gccatcgtcg atcgaagtcc
     5221 tgccggagat cgtcgatgtc gttggctcgc agatggaagt catgttcgac ggcggcattc
     5281 gctcaggcca ggacgtgatg cgcgcgctcg cgctcggcgc caaatcctgc atgatcggcc
     5341 gcgcctacat tcatggcctc ggcgccttcg gcggccccgg cgtcgccaag gcgatcgata
     5401 tcatcgccaa cgaactgagc gtgaccatgg ggctgtgcgg cgtcaacacc atcgccgaga
     5461 tcgacgatca cgtgctggcg gtttagaggc gaacgaaaat ctagtgtcgt cccggcttct
     5521 tcacttccct tctccccttg tgggagaagg tggccgtagg cggccttcgg ccgccgtcct
     5581 ttaagaggac gccgatgctt tgcatcggct atgcgaagtt cgtgccggat gagggggcgc
     5641 tttcgattcg ataggccgta cccctcaccc gtccgcgatg cttcgcatcg cgtccaccct
     5701 ctcccacaag gggagagggg aagaaagtat ctcacatcgg cggcgtaatg ccgtcgcggc
     5761 cgacattgac gcggttggct tccagcgtgc cgtcgtcttt ctttaccgcg ccgaagatga
     5821 tgatcttggc acccggcttc agttcggact tgtcgccggc gacgaaggtc acgatcggcg
     5881 tgtccggcgg caccacgact ttcttttcgc cgtccttgta tttcaccagg atgttctggc
     5941 cgtcggtgcc cttgaccgtc tcggcgacgg tggcgttggt catggttgag ttggggcgga
     6001 gatcccag
//
LOCUS       DASWGM010000444         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_308866,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000444 DASWGM010000000
VERSION     DASWGM010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308866"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="VGA15_12885"
     CDS             complement(<1..1636)
                     /locus_tag="VGA15_12885"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX9588621.1"
                     /translation="MAKAASKLKKTAAKSKPSVAARAKAAPPVARKALQKSPAKPAPK
                     PVSKPVAAKPAPETAKSGKWVFTFGDGKAEGKAELRDLLGGKGANLAEMANLGLPVPP
                     GFTIPTSACTYFYAHDKSYPKELKAQVEKALDHVGKLTGKAFGDAKNPLLVSVRSGGR
                     ASMPGMMDTVLNLGLNDKTVEALAEMSGDRRFAYDSYRRFITMYSDVVLGFEHHHFED
                     ILDTFKDGQGYSLDTDLTGDDWVDLVGRYKDAVARETGHDFPQDPHAQLWGAIGAVFS
                     SWMNARAVTYRRLHDIPESWGTAVNVQAMVFGNMGDSSATGVAFTRNPSTGESRLYGE
                     FLINAQGEDVVAGIRTPQDITEYARKESGSDKASMETAMPDAFKELTRIYTLLEKHYR
                     DMQDMEFTVEQGKLWMLQTRGGKRTAKAALRIAVELASEGLISKKEAVSRIDPASLDQ
                     LLHPTIDPSATRDVIATGLPASPGAAAGEIVFSSDEAAKLQADGRKVILVRIETSPED
                     IHGMHAAEGILTTRGGMTSHAAVVARGMGKPCVSGCG"
     gene            complement(1726..1881)
                     /locus_tag="VGA15_12890"
     CDS             complement(1726..1881)
                     /locus_tag="VGA15_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HEX9588622.1"
                     /translation="MTLTVAHISPIMSVIAGLLILIMPRLLNLVVAIFLILNGLIALG
                     LLKWLHM"
     gene            2114..2443
                     /locus_tag="VGA15_12895"
     CDS             2114..2443
                     /locus_tag="VGA15_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HEX9588623.1"
                     /translation="MRNRILAIAAIAGAISVPVAAQAQSVIIDDDVGIAVDQRPAFRE
                     YIVRERVPNYAIPDRVIVGGVLPETGVTYYDVPQTYGVMPYRYTVVNGRTVLVEPRSR
                     RIVQVVE"
BASE COUNT          542 a          930 c          931 g          593 t
ORIGIN      
        1 tgccgcagcc ggagacgcag ggcttgccca tgccgcgcgc gaccaccgcg gcgtgcgagg
       61 tcatgccgcc gcgcgtggtc agaatgcctt cggcggcgtg catgccgtga atgtcttccg
      121 ggctggtttc gatccgcacc aaaataacct tacgtccgtc ggcctgcagt ttcgcggcct
      181 catcggacga gaacacgatt tcgccggcgg cagcacccgg cgatgccggc agaccggtgg
      241 cgatgacgtc gcgggtcgca gacggatcga tggtcggatg cagcaattgg tcgagcgatg
      301 ccgggtcgat ccgcgacacc gcttccttct tggagatcag gccttcactg gcgagctcga
      361 cggcgatgcg cagtgcggct ttcgccgtgc gcttgccgcc gcgggtctgc agcatccaca
      421 gcttgccctg ctcgactgtg aactccatgt cctgcatgtc gcggtagtgc ttttccaaaa
      481 gcgtgtagat tcgcgtcagc tccttgaacg cgtcgggcat cgcggtttcc atcgatgcct
      541 tgtcggagcc ggactccttg cgcgcgtatt cggtgatgtc ctgcggcgtg cggatgcccg
      601 ccaccacgtc ttcgccctgc gcgttgatca ggaattcgcc gtacagcctg ctctcgccgg
      661 tggagggatt gcgcgtgaac gcaacgccgg tcgccgagct gtcgcccata tttccgaaca
      721 ccatcgcctg cacgttgacc gcggtgcccc aggactccgg aatgtcatgc agccgccggt
      781 aggtcaccgc gcgcgcgttc atccacgatg aaaacaccgc gccgatcgcg ccccacaatt
      841 gcgcgtgcgg atcctgcggg aagtcatggc cggtttcgcg cgccaccgcg tccttgtagc
      901 ggccgaccag atcgacccag tcgtcgccgg tcagatcggt atcgagcgaa tagccctggc
      961 cgtccttgaa ggtatcgaga atgtcctcga agtgatgatg ctcgaagccg agcaccacgt
     1021 ccgaatacat cgtgatgaag cggcggtagc tgtcataggc gaagcggcga tcgccggaca
     1081 tttcggccag cgcctcgacc gtcttgtcgt tgaggccgag attgagcacg gtgtccatca
     1141 tgcccggcat cgaggcgcgg ccgccggaac gtaccgaaac cagcagcgga ttcttggcat
     1201 cgccgaacgc cttgccggtc agcttgccga catggtccag cgccttttcg acctgcgcct
     1261 tcaattcctt cggataggat ttgtcgtgcg catagaaata cgtgcaggcc gacgtcggaa
     1321 tcgtgaagcc cggcggcacc ggtaatccga gattggccat ctcggcgaga ttggcgccct
     1381 tgccgcccag caaatcgcgc aactctgctt tgccctcggc cttgccgtcg ccgaaagtga
     1441 acacccattt accggatttc gccgtctctg gcgcaggctt ggcggcaacc ggcttgctga
     1501 cgggcttcgg cgccggcttg gccgggcttt tctgcaacgc cttgcgggcg accggcggcg
     1561 cggccttggc ccgggctgcc acggatggct ttgatttcgc tgcggttttc ttgagcttcg
     1621 atgcggcttt ggccatgact tacaatcaat ccgcgagagg aggtgagatg cgcgcttaca
     1681 ccacgttttg ccggttccgc gcaaggcgcg aagcctgttt caacgttaca tatggagcca
     1741 tttgagcagc ccgagggcga tcaggccgtt gagaatgaga aagatcgcaa ccaccagatt
     1801 gagcagccgc ggcatgatca gaatcagcag gcccgcaatc acggacatga ttggtgaaat
     1861 gtgggcgact gtgagggtca tgtaaaaact ctccgctatg gtttgggata tcgaatcgag
     1921 ccggacttta tccggtagat cggttccacg atagcagacg cgtgtcgctt caacgggttc
     1981 cagcgcctgc ataaaatgcc gaaaattgca gcggcaagat acgcctgttc ccgggaacga
     2041 tgccgcgcgc gatgaattgg gagggattgc gtgccggtgc aaaccggcat gcgtcatcgc
     2101 aaaggagttc accatgcgaa acaggatact ggccatcgca gccatcgcgg gcgcgatcag
     2161 cgtaccggtc gcggcacagg cgcagagcgt gatcatcgac gacgacgtcg gcatcgccgt
     2221 cgatcagcgg ccggcgttcc gcgaatacat cgtgcgtgag cgggtgccga actacgcgat
     2281 cccggatcgc gtgattgtcg gcggcgtgct gccggaaacc ggcgtcacct actatgacgt
     2341 gccgcaaacc tacggcgtca tgccctatcg ctacaccgtg gtgaacggcc ggaccgtgct
     2401 ggtcgaaccg cgctcgcgcc gcatcgtgca ggtggtcgag tgagggcctg aatgagttgt
     2461 ccgggcgccg gcgcatttta tgcgcgagag cgaccgggca tgaaagcccc gcccggttcc
     2521 cccgccgggc ggggcttttc ttttgaatca tcaccgggtg agtccttcgg cgcgaggcta
     2581 aggaactctt cgcgcacttc aagttccgat tcaattttca aacagccagg gcaattcagt
     2641 tgtagaaccg tcattgctat aatcccccac gcgacttcgt cctcgcgacg cgaagcgccc
     2701 gagacgttga tgaatcttcc gcccaagaga gggcgtgggg aacgccgggt gcccgctgca
     2761 cccgcagcct cgtgtgcact tgtagtagta gaacgcacac gagcaacaac gagtacaccg
     2821 gaatcacccg gcattcccgc acgcaatggt tttagcggct tatgtcgtgc tctccccggt
     2881 gaccgggctc ttttgccacc gtcgtccgcg gatatgtttt gtctaagccc ggtcgggccg
     2941 acgcaactcc gcgaacttga cgccagcgtc ggggcgtcag gaccacacga ttttgc
//
LOCUS       DASWGM010000445         5664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2124585,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000445 DASWGM010000000
VERSION     DASWGM010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124585"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1303
                     /locus_tag="VGA15_12900"
     CDS             170..1303
                     /locus_tag="VGA15_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588624.1"
                     /translation="MNGDPTLERIAQGNGLALCATGSWTARFAPVLEQMVADAERLTG
                     SRPDIFIDVSKVSKLDTFGAWLIERLRRSLAHGGVEARIAGLSANYSSLVDEVRRVKA
                     TAVVDTTSVTITGVLDQIGRNMAGFGTTLIGLIDMLGAVLAAVGRVVIHPSGFRLTST
                     VHHLEQVCWRAVPIVLLITFLIGCIISQQGIFHFRKFGADIFVVDMLGVLVLREIGVL
                     LVAIMVAGRSGSAYTAELGSMKMREEIDALRTMGFDPIEVLVLPRMLALIIALPILAF
                     LGAMAALVGGGLVANFYGGVDPEAFMLRLRDAISIDHFIVGIVKAPVMAAVIGIVACV
                     EGLAVEGSAESLGQHTTSSVVQGIFFVIVMDGVFAIFFASIGM"
     gene            1306..2076
                     /locus_tag="VGA15_12905"
     CDS             1306..2076
                     /locus_tag="VGA15_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588625.1"
                     /translation="MAPEIPDAIIRVRDISVRFGATKVLDGLNLDVKRGEILGFVGPS
                     GAGKSVLTRTIIGLVPKVSGRIEVFGIDLDAANAAARRGVERRWGILFQQGALFSSLT
                     VRQNIQFPVREYLNVSQRLLDEITVAKLGMVGLRPEVASRFPSELSGGMIKRVALARA
                     LALDPELVFLDEPTSGLDPIGAGDFDELVRTLQRTLGLTVFMVTHDLDSLYTACDRIA
                     VLGNGKIIAAGSMADMQASEHPWLRAYFHGKRARAVMG"
     gene            2096..2983
                     /locus_tag="VGA15_12910"
     CDS             2096..2983
                     /locus_tag="VGA15_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9588626.1"
                     /translation="METRANFVLIGSFTLAVIAAAFGFVLWFQSLHTTKARSPLRVVF
                     EGPATGLRNGGSVNFNGIRIGEVISVKLDNPRRVVALAMVENNAPIRKDTLVGLEFQG
                     LTGVAAISLKGGEEAAPGVPLDEDGVPVLTADPNALQDVTEAIRGTLQNINRVVADNQ
                     ETVKASLKNLETFTAALARNSEKIDNVMLKVDGVVGKADNLMLGLNTLAGGKEGGELF
                     QAVKSIRELAEDFDKRSGALMSDGRRTLGDISRAVNNLDRNPTRLLFGAGNSNSNAAA
                     APAPAAGPTPAAAAEKRRP"
     gene            3155..3553
                     /locus_tag="VGA15_12915"
     CDS             3155..3553
                     /locus_tag="VGA15_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588627.1"
                     /translation="MAVIRLILGAALLVTALGLIFELFVPGSGRMIDIGAVVFGLATW
                     IVPPLLMALSRNKKRSSLESVGFTIYLLVVAACLSAIGLSLALLTLVFAQDANWAWLG
                     LLAIAAFWVSGGIGIYVSQRRHRRAREVSK"
     gene            3958..4269
                     /locus_tag="VGA15_12920"
     CDS             3958..4269
                     /locus_tag="VGA15_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588628.1"
                     /translation="MLTALLVATYVIVGFGGEISCAEETLATANSLDASAVTDKSDEG
                     SKKAPDVVEHCYTCVPLLMPALVLVAEPSAEPVKLSFEAPTFLLEDHPWVDTPPPKHL
                     T"
     gene            4329..5603
                     /locus_tag="VGA15_12925"
     CDS             4329..5603
                     /locus_tag="VGA15_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9588629.1"
                     /translation="MYKLGVALRLMCALLVVTIPVEAAERALRSISLAQALQRALAAN
                     PRLTAAERDIGIATGLKVQAGALPNPELSFELDNALGSGRYKGLQSAETNLQLSQLVE
                     LGGKREARLAGGDAGIGTAVWQRRATRLEVLSETAIAFITVVSAQRRIEIFDEQISSL
                     DQLIPLLQKRVQEGASSPAETLRAQVAADLFRVDRERARTQLATARRDLAILTGDSSP
                     RFGEAVGRLANVGQPPSFQSVLQAIEANPQLMRWTAVTAQRNAELILARLKPIPDVHL
                     SAGWRHFQDTDDNAVRLGVSIPLPVLDQNTGNIIAAEEVLAKTGAERAINKLVLISIA
                     GRAYDALNGALAELKLLRTSVIPNARNASETIQSGYLQGRFTLLELLDVRGSVLQALL
                     REQEALQNFHIAIATIEGLVGSPFSLTRESSR"
BASE COUNT         1025 a         1685 c         1800 g         1154 t
ORIGIN      
        1 gacaagcttt gggaatttgg tggaagtcat tttaaaatct ggcaatttct gaaccggccg
       61 gcttgcccgg cgcaaccaag tatggctggt tagggatgca ttccacaagg cggcgtgttg
      121 gaactttccc ctccatgaat agcggagaat cagcacagga gttggcatcg tgaacggcga
      181 cccgacattg gagcggattg cacaaggcaa cggactggcg ctgtgcgcta cgggttcgtg
      241 gaccgcccgg tttgcgccgg tgcttgagca gatggtggcg gacgccgaaa ggctgaccgg
      301 aagccggccc gacatcttca tcgatgtgtc gaaggtctcc aaactcgaca ccttcggggc
      361 ctggctgatc gaacggcttc gccgcagcct ggcccatggc ggcgtcgagg ccaggatcgc
      421 cgggctttcg gcgaactatt cgagtctggt cgacgaagtg cgccgggtca aagcgaccgc
      481 cgtggtcgat accacgtcgg tgaccattac cggcgtgctg gatcaaatcg gccgcaacat
      541 ggccgggttc ggcacgacgt tgatcggact gatcgacatg ctcggcgctg ttctcgccgc
      601 ggtcgggcga gtggtgattc atccgtccgg gttccgcctg acctcgaccg tgcatcatct
      661 cgagcaggta tgctggcggg cggtgccgat cgtgctgctc atcacctttc tgatcggctg
      721 catcatctcc cagcagggca tctttcattt ccgcaaattc ggtgcggata ttttcgtggt
      781 cgacatgctg ggcgtgctgg tgctgcgcga aatcggcgta ctgctggtgg cgatcatggt
      841 ggcgggccgc tcgggcagcg cctataccgc cgaactcggt tcgatgaaaa tgcgcgagga
      901 gatcgacgcg ctgcgcacca tggggttcga cccgatcgag gttctggttc tgccgcggat
      961 gctggcgctg atcattgcgc tgccgattct ggcctttctc ggcgccatgg cggcgctggt
     1021 gggcggcggg ctggtggcga acttttatgg cggtgtcgat cccgaggcgt tcatgcttcg
     1081 gctgcgcgac gcgatttcga tcgatcactt catcgtcggc atcgtcaagg cgccggtgat
     1141 ggcggcggtg atcggcatcg tcgcctgcgt cgagggtctg gcggtcgagg gcagcgccga
     1201 gtcgctcggg caacacacca cctcctcggt ggtgcagggg attttcttcg tcattgtgat
     1261 ggatggcgta tttgcgatct ttttcgcctc gattggaatg tgacgatggc gcctgaaatt
     1321 cccgacgcca tcatccgggt gcgcgacatc tcggtgcggt tcggcgccac caaggttctg
     1381 gacgggctca atctcgacgt gaagcgcggc gagatcctgg gcttcgtcgg tccgtccggc
     1441 gccggcaagt cggtgctgac gcgcaccatc atcggcctcg ttcccaaggt cagcggccgg
     1501 atcgaggtgt tcgggatcga tctcgacgcc gccaacgccg ccgcgcgacg cggggtcgag
     1561 cggcgctggg gcatcctgtt ccagcagggc gccttgtttt cctcgctgac ggtgcggcag
     1621 aatatccagt tcccggtgcg cgaatatctc aacgtctcgc agcgcctgct cgacgaaatc
     1681 accgtggcca agctcggcat ggtggggttg cgccccgaag tcgccagccg ttttccctcg
     1741 gaactgtccg gcggcatgat caagcgcgtg gcgctggccc gcgcgctggc gctcgacccc
     1801 gaactggtgt tcctcgatga gccgacctcg ggcctcgatc ccatcggcgc cggtgacttt
     1861 gacgagctgg tccggaccct gcagcgcact ttgggcctga ccgttttcat ggtaacccac
     1921 gatctcgaca gcctatatac cgcttgcgac cgtatcgctg ttttagggaa cggtaagatc
     1981 attgccgcag gatcgatggc cgacatgcag gcctcagagc atccctggtt gagggcgtat
     2041 ttccacggca agcgcgcccg cgcggtcatg ggctagtgat accggagttt atggaatgga
     2101 aacgcgggcg aacttcgtcc tgatcggatc gttcacgctg gcagtgattg ccgcggcgtt
     2161 cggctttgtg ctgtggttcc agtccctcca caccaccaag gcgcgcagcc cgctccgcgt
     2221 cgtgttcgaa ggtccggcaa cgggtttgcg caacggcggc agcgtgaatt tcaacggcat
     2281 ccggatcggg gaagtgatct ccgtgaagct ggacaacccg cgtcgagttg tcgcactcgc
     2341 gatggtcgag aacaacgccc cgatccgcaa ggacacgctg gtcgggctcg aatttcaggg
     2401 gttgaccggg gtcgctgcga tatcgctgaa gggtggcgag gaggcagcac ccggtgttcc
     2461 cctcgacgag gatggcgtgc cggtgctgac cgccgatccg aacgcgctgc aggacgtcac
     2521 cgaggcgatc cgcggcaccc tgcagaacat caaccgggtg gttgccgaca accaggagac
     2581 ggtcaaggcc tcgcttaaaa acctcgagac gtttaccgcc gcgctggcgc gcaattccga
     2641 gaagatcgac aacgtcatgc tgaaagtcga cggcgtcgtg ggcaaggccg acaacctcat
     2701 gctgggtctc aatacgctgg ccggcggcaa ggagggcggc gaactgttcc aggcggtgaa
     2761 atcgatccgc gaactggcgg aggatttcga caagcgatcg ggcgccttga tgtccgatgg
     2821 ccgccgcacg ctcggcgaca tcagccgcgc ggtgaacaat ctcgaccgca acccgacccg
     2881 cctgctgttc ggcgcgggca acagcaacag caacgccgcg gcggcaccgg cgcctgcggc
     2941 gggtcctacg cccgctgcgg cggcggagaa gaggcggccg tagcgtcgaa ctcgcaggtg
     3001 ggttagcgaa cgggccgcgc gaaatgcgcg cccgatgata aactccggcg atgccaacgc
     3061 ggcgcaagac gcgtagctca tgatcgggcg aacgttcgaa ccgatttgcg ccagcctgcg
     3121 acgaagttac gcgtccagga ggcatcgcga taacgtggca gtcattagac taatccttgg
     3181 ggccgcattg ctggtgacgg cactcggcct gattttcgaa ttgtttgtac ccgggtccgg
     3241 tcgcatgatt gatatcggcg ctgtggtgtt cggcctcgcg acctggatcg ttccaccgct
     3301 tctgatggct ttatcccgga acaaaaagcg gtcatcgctg gagagcgtcg gcttcacaat
     3361 ctatcttctt gttgtggccg cctgtctctc tgctatcggt ctttcactgg cgcttctcac
     3421 cctcgttttc gcgcaagatg caaactgggc ctggctcggt ctgctggcca ttgcggcgtt
     3481 ctgggtatca gggggaatcg ggatctatgt cagtcaacgt cgccatcggc gggccagaga
     3541 agtttccaag taggtagttg agaaatggaa tctttttcaa aaggggctgg acagacccgt
     3601 caccagatcg aacagcgaag gcagaaggtg taacccgcct aatcgcggcc aggcttgatc
     3661 aagctgcact tcgaacatct gcactgcgac gggcagccaa tatcttgcaa tgaaggaagt
     3721 tgtctccacc aagaatatgt ttctaccgga cgccgctatc ggctcatgaa gctattacgg
     3781 ccgcaataac gactgatcca atggcgtctt ctcggcttgc ctgcgaacgc ccgtgcgttg
     3841 cacccggact ctggagctga atcgtaatag tgttgcgcca caaagtttga tattcctagc
     3901 ccgatgaggc aaacagccgc ccatagacgt gctccccgtg catcggcttt tcgccgggtg
     3961 ctgacggctt tgcttgtcgc gacatacgta atcgttgggt ttggtggtga gatttcttgc
     4021 gcagaggaaa cgctggcaac cgctaactca cttgacgcaa gcgcggtgac ggacaagtcc
     4081 gacgaaggct cgaagaaggc tcctgatgta gttgagcatt gttacacctg cgttcctctt
     4141 cttatgcccg cgcttgtact cgttgccgag ccttcggccg aaccggtcaa actatccttc
     4201 gaagccccga cgttcctgct ggaagaccat ccctgggttg acacgccacc ccccaaacat
     4261 ttgacatgaa aatcgtaatg cgagcggcgc gagccgcgtc gctgtcttac cgcttttccg
     4321 gttgaatgat gtataagctc ggagtggctt tgcgcctgat gtgcgcattg cttgttgtca
     4381 caattccagt cgaagctgct gagagggcgc tgcgatcgat ttcgttggcc caggcgctgc
     4441 aacgcgcttt ggcggcgaat ccgcgcctca cggcggcgga gcgcgatatc ggcattgcga
     4501 ccggcctcaa ggttcaggcc ggcgccctcc cgaatcccga actttcgttc gaactcgaca
     4561 acgcgctcgg ttctggaagg tacaaaggac tgcagtcagc cgagacaaac ctccaactca
     4621 gccagcttgt tgaactcggc ggcaaacgag aggcgcgact ggctggcggt gatgcaggta
     4681 tcggcaccgc ggtttggcag cggcgggcaa cccggttgga ggtgctgtcg gagaccgcca
     4741 tcgccttcat cacggtggtg agcgcgcagc ggcggattga aatcttcgac gaacagattt
     4801 cgagcctcga tcagctgatt ccgctgttgc aaaagcgtgt tcaggagggc gcgtcctctc
     4861 cggcagaaac attgcgggcg caagtggcgg ccgacttgtt ccgcgtcgat cgcgagcggg
     4921 ccaggacaca actggccacg gcgcggcgcg acctcgcgat cctgacgggc gacagcagcc
     4981 cccgcttcgg cgaagcggtg gggcgtctcg ccaatgtcgg ccagccgccg tcgtttcagt
     5041 cggttcttca ggcgatcgag gctaacccgc aactgatgcg ctggacggcc gtaacggcgc
     5101 aacgcaatgc cgagcttatc ctggcgcggc ttaagccgat ccccgacgtt cacctctccg
     5161 cggggtggcg gcatttccag gacaccgacg acaacgcggt gcggctgggc gtatcgatac
     5221 cgcttccggt cctcgatcag aacaccggaa acatcattgc cgccgaggaa gtgctggcaa
     5281 agaccggcgc cgagcgtgcg atcaacaagc ttgtcctgat cagcatcgcg ggccgcgcct
     5341 acgacgcgtt gaatggcgcg ctcgccgagc taaagctgtt gcggacgtcc gtcatcccaa
     5401 atgcgcgcaa tgcctcagaa accatccaga gcggctacct gcagggccgc ttcacgctgc
     5461 tcgagctgct tgacgttcga ggttcggtcc tgcaggcact gctccgcgag caggaagcgc
     5521 tgcagaattt ccacattgcc atcgcgacga tcgaaggcct cgtcggtagc ccgttttcgc
     5581 tgacccgcga gagttcaaga tgatccaagg attgatccga tatttggtct tcgttctggt
     5641 tgccgcggcg cttgtctatg gcgg
//
LOCUS       DASWGM010000446         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2266812,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000446 DASWGM010000000
VERSION     DASWGM010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2266812"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="VGA15_12930"
     CDS             <1..158
                     /locus_tag="VGA15_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEX9588630.1"
                     /translation="LAATVQTKTVVPCQPCHRPVCTMNDHRCMRDIPASDVVATIRRV
                     LTGGIRK"
     gene            155..697
                     /locus_tag="VGA15_12935"
     CDS             155..697
                     /locus_tag="VGA15_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9588631.1"
                     /translation="MSGIDPAGPTPKPAAFLDRDGVINHDDGFMGTQDRIRWMPNAAK
                     AIRRLNDAGYLVFLFTNQSGVARGFFAEDDLGTLHDWMRAELTTQGAVIDDIRYCPHH
                     PGGSVAGYLEDHHWRKPSPGMILDLMHHWPVRRAGSFVIGDRSTDIEAAEAAGLPGFL
                     FSGGDLDAFVADVIAQTSAR"
     gene            complement(679..1725)
                     /locus_tag="VGA15_12940"
     CDS             complement(679..1725)
                     /locus_tag="VGA15_12940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX9588632.1"
                     /translation="MAIENNSADNLQLIVPNLHRRYSGVTATNRMVAPKLAKMFRAAW
                     LGPDAPDGIARMGILDLAKLWHRRNSLIWHARRNNEMIVGVLLRSLGWPLKLVFTSAA
                     QRHHSWITRWLIRRMDAIIATNDISASFLKRDATVIPHGVDTKLYKPPVDRAAAFAQA
                     GLPGRYAIGCFGRVRAQKGSDVFVDAMCRLLPRYPDFTAILVGAITPEHTAFTNQLKK
                     RIEAAGLQSRIVITGELGIEEVQRWYQRLTIYAFTSRNEGFGLTLIEAMSVGTALVAS
                     RAGAAELVVEDGVTGVLTPPGDVDALVAALEPLMRDPASAIAMGIRAQARVLAKFSLD
                     AEANRIAEVYRALV"
     gene            1850..>2134
                     /locus_tag="VGA15_12945"
     CDS             1850..>2134
                     /locus_tag="VGA15_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9588633.1"
                     /translation="MTVLVTGGAGYIGSHMVQALAEAGEKVVVIDNLSTGFSEFLPEG
                     VPLFIGDAADENLVEGVIAAHGVESIIHFAGSVVVPDSMRDPLAYYRNNTM"
BASE COUNT          399 a          685 c          643 g          407 t
ORIGIN      
        1 ggctggcggc gacggtacag accaaaaccg tcgtgccctg ccaaccctgc caccgcccgg
       61 tctgcaccat gaacgaccat cgctgcatgc gcgacattcc cgcatccgac gtggtcgcca
      121 ccatcaggcg cgtgttgacg gggggcataa gaaaatgagc ggtatcgatc cggccggtcc
      181 cacgccgaag cccgccgcgt tcctcgatcg cgacggcgtc atcaaccatg acgacggctt
      241 tatgggaacg caggaccgga ttcgctggat gccgaacgcg gcgaaggcga tccgccgcct
      301 gaacgatgcc ggatatttgg tgttcctctt caccaatcaa tccggcgtgg cgcgtggatt
      361 ctttgcagag gacgatctgg gcacgctgca cgactggatg cgcgccgagc tcaccactca
      421 aggtgccgtc atcgacgata tcagatattg cccgcaccat ccgggcggat cggttgccgg
      481 ctatctcgag gaccaccatt ggcgaaagcc gagccccggc atgattctcg atctgatgca
      541 ccactggccg gtgcggcgcg caggcagttt tgtcattggc gatcgttcga ccgatatcga
      601 agccgcggaa gccgccggat tgccgggctt tcttttctcg ggcggcgacc tcgacgcgtt
      661 tgtggcggac gtgatcgctc agacaagcgc gcgatagacc tcagcgatcc ggttggcttc
      721 cgcgtccagg ctgaatttcg ccagcacccg tgcctgcgcg cgtatcccca tcgcaatggc
      781 cgacgcagga tcgcgcatca gcggctccag cgcggcgacg agcgcatcaa catcgcccgg
      841 tggcgtcagc acgccggtca ccccgtcctc gaccaccagt tcagcagcgc cggcgcgcga
      901 cgccaccagc gccgtaccca ccgacatcgc ctcgatcagc gtcagaccga atccttcgtt
      961 gcgcgaggtg aaagcgtaga tcgtcagccg ctggtaccag cgctgtactt cctcgattcc
     1021 caattcccct gttatgacga tgcgcgattg cagtcccgca gcctcgattc gctttttcag
     1081 ctggttggtg aaagcggtgt gctccggcgt gatcgcgccg accaggatag cggtgaaatc
     1141 aggatagcgc ggcagcaacc tgcacatcgc atccacaaac acatcgctgc ctttttgcgc
     1201 gcgcacccgt ccgaagcagc cgatcgcgta gcggcccggc aacccggctt gcgcgaatgc
     1261 cgccgcacga tccactggcg gcttgtagag tttggtgtcg acgccgtgcg gaatgacggt
     1321 ggcatctcgt ttgagaaacg acgcggagat atcatttgtg gcgatgattg cgtccatcct
     1381 cctgatcagc cagcgcgtga tccagctgtg atggcgctgc gccgccgagg tgaacaccag
     1441 cttcagcggc cagccgagcg atcgcaacag cacgcctacg atcatctcgt tgttgcgccg
     1501 cgcgtgccag atcagggagt tgcgtcggtg ccacaatttc gcaagatcca ggattcccat
     1561 tcgcgcgatg ccatccggcg catcgggacc cagccatgcc gcgcggaaca ttttcgcgag
     1621 tttcggcgcc accatgcgat tggtcgcggt cacgcccgaa taacgcctgt gcagattagg
     1681 cacgatcagc tgcagattgt cggcggaatt gttctcgatg gccacggcag gctccgttac
     1741 gcgcattcct atacgcaaca ttaagcatag tggccagttc gccagggttg aaactcactc
     1801 ttcaccgcgc tgtgggtaca tcgtcccaaa tcgggagatg ggtaagctca tgaccgtgct
     1861 tgtcaccggc ggcgccggct atatcggaag tcacatggtt caggcgctgg ctgaagccgg
     1921 cgaaaaagtc gtggtgatcg acaacctgtc cacgggcttt tcggagttct tgcccgaagg
     1981 cgttccgctg ttcatcggcg acgccgcgga tgagaacctg gtcgagggcg tgatcgcagc
     2041 gcacggcgtg gaaagcatca ttcacttcgc gggctcggtg gtggtgccgg actcgatgcg
     2101 cgacccgctc gcctattacc gcaacaacac catg
//
LOCUS       DASWGM010000447         6179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_50605,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000447 DASWGM010000000
VERSION     DASWGM010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50605"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..259
                     /locus_tag="VGA15_12950"
     CDS             23..259
                     /locus_tag="VGA15_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9588634.1"
                     /translation="MGSLSIWHWIVVIAVVLLLFGRGKISDLMGDVAQGIKAFKKGMQ
                     DDEKTAEKPEPVKTIDHNAAPSTAARSDVGSKAV"
     gene            388..1008
                     /gene="tatB"
                     /locus_tag="VGA15_12955"
     CDS             388..1008
                     /gene="tatB"
                     /locus_tag="VGA15_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX9588635.1"
                     /translation="MFDIGWSELVVIAVVALIAIGPKELPGVLRMVGQWMGKARKMAA
                     EFQGQFQEAMREAEMADLKKSFDEVKEAATGFASGNVMTSLQKDVGDALRIDDIDKPA
                     TSASDAPATSASDALAIEPPVATSAIDTTATDTPSVSDAPVTSASDAPVTPTTREPPI
                     PETFTEADAHAAAMEPLAITREAQPLPAPQDITPAAPEVLKDVKAS"
     gene            1005..1832
                     /gene="tatC"
                     /locus_tag="VGA15_12960"
     CDS             1005..1832
                     /gene="tatC"
                     /locus_tag="VGA15_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX9588636.1"
                     /translation="MSAEDIEASKAPLMDHLIELRSRLIKALLAFGIAFIFCFFFAKQ
                     IYNVLVWPFVWVAGAENSKFIYTALLEYFLTQLKLALFGAGFISFPIVAAQIYKFVAP
                     GLYKHERGAFLPYLIATPFFFVLGSMLVYFVVLPMLVRFSLGMQQAAGAESAQIQLLP
                     KVGEYLSLMMSLIFAFGVAFQLPVILTLLGRIGIITAKQLRDKRRYFIVIAFIIAAVL
                     TPPDVISQASLALPLLALYEGSILAVAMVEKKAAAAKAASGTPPSAAPPPPAANPAE"
     gene            2073..3437
                     /gene="serS"
                     /locus_tag="VGA15_12965"
     CDS             2073..3437
                     /gene="serS"
                     /locus_tag="VGA15_12965"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX9588637.1"
                     /translation="MHDIKWIRENAHSFDAALKRRGLEPLSSSLLAIDERRRAAILKS
                     EQAQARRNSASKEIGDAKKAKDDARAATLMAEVAELKTTMPQLEAATKAADEELAKEL
                     AAIPNLPLDEVPDGADEHGNVVRHHFGAVRHYAFAPKPHDDLGGALGLMDFEAAAKLS
                     GARFVVLKKGLARLDRAIGQFMLDLHTNEHGYTEINPPLLVRDDVMFGTGQLPKFEDD
                     QFWAVKGELLTVADESAVAGLGKLKSERLGLIPTAEVPLTNLVRESILNEKELPMRLT
                     ALTPCFRAEAGAAGRDTRGMIRQHQFTKVELVSITTPETSKDEHERMLACAEEVLRQL
                     DLHYRVMTLCAGDMGFAAQKTYDIEVWMPGQFSSQGEGGAYREISSCSVCGDFQARRM
                     DARYRGPDGKPRFVHTLNGSGTAVGRALIAVMETYQQEDGSIAVPEVLQRYMGDVKVI
                     APDK"
     gene            3528..3905
                     /locus_tag="VGA15_12970"
     CDS             3528..3905
                     /locus_tag="VGA15_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588638.1"
                     /translation="MPLHRDIYWVGRQWAVTGYGMQAIDQRLNGMFDIEVSRLWEDGL
                     IERMCGLKWINILDFDKGLAAARKRYPEPPRKASPPDATVSSSKDGSSIDQLKQVPIF
                     DMRSQGGTAKAKFLSLWRVHRPR"
     gene            4690..5379
                     /locus_tag="VGA15_12975"
     CDS             4690..5379
                     /locus_tag="VGA15_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9588639.1"
                     /translation="MPNSPDVREKDVFDPKAFLGRIDAGKTIERYGKNQKIFSQGDVA
                     DSVYFIRKGKVKITVLSEHGKEAVVGIFGEGQFFGEGCLMGAQLRSTTGAAMEECLIT
                     SISKSAMLATLGAEPKFSAFFMAYLLRRNSRIEDDLIDQLFNSSERRLARLLLLLANF
                     GEEHGKQPIAITLSQETLAEMIGTTRSRVSFFMNKFRKQGYIDYNGKIEVHRSLLDVV
                     LRDKPQIQQDE"
     gene            5482..5745
                     /locus_tag="VGA15_12980"
     CDS             5482..5745
                     /locus_tag="VGA15_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588640.1"
                     /translation="MPQYRFQVRRGKFSNGSSVEATLEDSEAAWKEAAAICADLARDI
                     IRADPEWLLEVTDEAGQGLFNFRFAAESLQPKVGADLHAVLTH"
BASE COUNT         1287 a         1800 c         1900 g         1192 t
ORIGIN      
        1 catgtgtttg gagggttgca ggatgggttc gcttagcatt tggcactgga tcgtggtgat
       61 cgcagtcgtc ctgctgttgt tcggccgcgg caagatttcc gacctgatgg gcgacgtcgc
      121 gcagggcatc aaggccttca agaagggcat gcaggacgac gagaagactg cggaaaagcc
      181 cgagccggtg aagaccatcg atcataacgc ggcgccttcc acggcagcgc gctcggatgt
      241 cggcagcaag gctgtctgac cagcatcttg cggcaggctt gcagcaggat gggttgaggg
      301 agccgcgagc gactgccaag cctcggtcgt ccaagcctcg gccgtccaag tctcggccgt
      361 ccaagtctcg cgtgagcgga ttttttcatg ttcgacatcg ggtggagtga actggtcgtc
      421 atcgcggtcg tcgcgctgat cgccatcggc ccgaaagaac tgccgggcgt gctgcgcatg
      481 gtcgggcaat ggatgggcaa ggcgcgcaag atggccgccg aattccaggg ccagttccag
      541 gaagcgatgc gcgaggccga aatggccgac ctcaaaaaga gttttgacga ggtcaaggaa
      601 gccgcaaccg gctttgccag cggtaatgtc atgacctcgc tgcaaaagga cgtcggcgac
      661 gcgctgcgaa tcgacgacat cgataagccc gcgacgtcgg caagcgacgc accggcgacg
      721 tcggcaagcg acgcgctggc gatcgagccg ccggtcgcga catcggccat cgacacgacg
      781 gcaaccgaca cgccctcggt aagcgacgca ccggtgacgt cggcaagcga cgcaccggtt
      841 actccgacaa cacgggaacc gccgataccg gagaccttca ccgaggcgga cgcccacgcc
      901 gccgcgatgg agccgctggc gatcacgcgc gaggctcaac ccctgcctgc gccgcaggac
      961 ataacgcccg cggcacctga ggtcctcaag gacgtcaaag cgtcatgagc gccgaggata
     1021 tcgaagccag caaagcgccg ttgatggatc atttgatcga gctgcgctcg cggctgatca
     1081 aggcgttgct ggcgtttggt atcgccttca tcttctgttt cttcttcgcc aagcagatct
     1141 acaacgtgct ggtgtggcca ttcgtctggg tcgccggcgc ggaaaactcg aaattcatct
     1201 acacagcact tttggaatat ttcctgaccc agttgaaact ggcgctgttc ggcgccggct
     1261 ttatttcgtt tccgatcgtg gcggcgcaga tctacaaatt cgtggcgccc ggcctctaca
     1321 agcacgagcg cggcgcgttc ctgccgtatc tgatcgccac cccgttcttc ttcgtgctgg
     1381 gatcgatgct ggtgtatttc gtggtgctgc cgatgctggt gcggttctcg ctcggcatgc
     1441 agcaggccgc tggcgccgag agcgcgcaga tccagttatt gcccaaggtc ggcgaatatc
     1501 tgtcgctgat gatgtcgctg atcttcgcgt tcggcgtcgc gttccagctg ccggtgatcc
     1561 tgacgctgtt ggggcggatc ggcattatta ccgcgaaaca gctgcgcgat aagcgccgct
     1621 atttcatcgt gatcgccttc atcatcgcgg cagttctgac gccgcccgac gtcatcagcc
     1681 aggcctcgct cgcattgccg ctattggcgc tgtacgaagg ctcgatcctc gcggtggcca
     1741 tggtggagaa aaaagcggct gccgccaagg ctgccagcgg tacgccgccc tccgccgccc
     1801 caccgccgcc ggccgccaat cccgcggagt aggctcaacg ccaccgcaca caccgccgtc
     1861 atcctgaggg cagcccaaag ggccgcgtct cgaaggatgg gccgcaagca cgtgtagccg
     1921 catccttcga ggcgcgccaa ggggctcgca cctcaggatg acgactcgta cggcgccttc
     1981 ggtgttccaa atcaccctta ttcgggttac tggatgcccg ccttcgcggg catgacgcct
     2041 ccagacttgc gacgccacag gaccgaacac ccatgcacga catcaaatgg atccgcgaaa
     2101 acgcccactc tttcgacgcc gcgctgaaac ggcgcgggct ggagccgttg tcctcgtcgc
     2161 tgctggcgat cgacgaaagg cgccgcgcgg ccatcctgaa atcggagcag gcgcaggcgc
     2221 ggcgcaattc ggcctcgaag gaaatcggcg acgccaagaa ggccaaggac gacgcgcgcg
     2281 cagcaacgtt gatggccgaa gtcgccgagc tcaagaccac catgccgcaa ctcgaggccg
     2341 cgaccaaggc agcggacgag gagctcgcca aagagctagc cgcgattccg aatttgccgc
     2401 tggacgaagt ccccgacggc gccgacgagc acggcaatgt ggtgcggcac catttcggcg
     2461 cggtgcgcca ttatgcgttc gcgccaaaac cgcatgacga tctcggcggc gcgctcggct
     2521 tgatggattt tgaagccgcg gcaaaactca gcggcgcgcg ttttgtggtg ctcaagaaag
     2581 gcctcgcgcg gctcgatcgc gcgatcggcc aattcatgct cgacctgcac accaacgagc
     2641 acggctacac cgaaatcaat ccgccgctgc tggtgcgcga cgatgtgatg tttggaacgg
     2701 gtcagctgcc taagtttgaa gatgaccagt tctgggccgt caagggggaa ttgctcactg
     2761 ttgccgatga gagtgctgtt gcgggcctcg gcaaactaaa gagcgagcgc cttggtctca
     2821 tccccaccgc cgaagtaccg ttgaccaatc tcgttcgcga atccatcctc aatgagaaag
     2881 aattgccgat gcgtttgacg gcgctgacgc cgtgctttcg cgcggaagcg ggggcggctg
     2941 gacgcgatac ccgcggcatg atccggcagc atcagttcac caaggtcgaa ctggtctcga
     3001 tcacgacacc tgagaccagc aaggacgagc acgagcgcat gctggcctgc gccgaggaag
     3061 ttttgcggca gctcgatctg cattaccgcg tgatgacgct gtgcgccggc gacatggggt
     3121 ttgcggcgca aaagacctac gatatcgagg tctggatgcc aggacaattt tcgagtcagg
     3181 gcgagggcgg cgcctaccgc gaaatttcga gctgctcggt gtgcggcgat ttccaggcgc
     3241 ggcgcatgga tgcgcgctat cgcgggcccg acggcaagcc gcgcttcgtg cacacgctca
     3301 acggttccgg caccgcggtc ggccgtgcgc tgatcgcggt gatggaaacc taccagcagg
     3361 aagacggctc gatcgcggtg ccggaagtgc tgcaacgcta tatgggtgac gtgaaggtca
     3421 ttgcccccga caagtaattt tgccgctttc catattgcga aggtggcgcg aagcggtatc
     3481 ttcgtgttct tgcccgggct gggtctcgca ggaagcgaac ttcgaaaatg ccgttgcatc
     3541 gcgacatcta ttgggtcggc aggcagtggg ctgttaccgg ctacgggatg caggcgatcg
     3601 atcaaaggct gaatggcatg ttcgatatcg aggtctcccg tctttgggaa gacggcctca
     3661 ttgaacgcat gtgtgggctg aaatggatca atatcctgga tttcgacaag ggacttgccg
     3721 ccgcccgcaa gcgttacccg gaaccgcccc gaaaggcctc gccgcctgac gcgactgttt
     3781 ccagctcgaa agatggcagc tccattgatc aactgaaaca ggttccaata ttcgacatgc
     3841 ggagccaggg cgggacggcg aaagcaaaat tcctctccct ttggcgtgtg catcgtccgc
     3901 gataacggtc tggtcgtcgc aaacgaaaaa gcccgcattt gaaatggcag tggccggaat
     3961 tgccgctcgg cctgttcacc cctcgctgaa cgaagttttt caaaatgagc aattttgaac
     4021 acggcatgag acgcgggtga gtccattcta agtgctcacc cttgcgtgct caccgccgtt
     4081 tcggtgtagc gcgttcggtg acgctcccaa ctcaaagaag gacgcggcat gcccgaatat
     4141 cagccggtat cttcagcggc catttccgcg atcgagcggc cgcgctgccc gaaatgcaac
     4201 cagaatcgca tgctgctggc gaggctcgag gtgggagcgc ccggcatcga gtaccggaca
     4261 ttcgagtgcc agaagtgcgg tcgcgttcac agggcggtgg tatccagcga tcccatgaac
     4321 tctgagatga gcggctggct tgccggtgaa ttaaggcggc cgacctagag catgacgcgg
     4381 aaaagtggaa cccggttttc cgaaaagatc atgctcaaac aacatgctca aacagagaga
     4441 tgagatcatg gatcggattc aatgtaatcg gatcatgatc tagcgcgcag cggtgaagcg
     4501 gctgaggctg tttattcccg cgtgaggcta catccttaca tgaactcacc acgagttgag
     4561 ttgtcattcc gcggcaattt atgtcgtcta ccgcacataa cggggctata atggtgcatt
     4621 accgcgccgg cttggggtct tgccggcgag cgagagtgtt gtgctcccgc ccgattacgg
     4681 aacaacacca tgcccaattc gccggatgtt cgggaaaagg acgtgtttga ccccaaggcg
     4741 tttctgggcc gtatcgatgc gggaaaaacc atcgagcgat acgggaagaa ccagaagata
     4801 ttcagccagg gagacgtcgc cgacagcgtc tacttcatcc ggaaaggcaa ggtcaagatc
     4861 acggtcttgt ccgagcacgg caaggaagct gtggtcggga ttttcgggga gggtcagttc
     4921 ttcggcgaag gctgtctgat gggagcgcaa ttgcgcagca cgaccggcgc ggccatggaa
     4981 gagtgcctga tcacatcgat atcaaaatcc gcaatgcttg caacgcttgg tgcggagccg
     5041 aagttttccg catttttcat ggcctacctg ctgagacgaa atagccggat cgaagacgac
     5101 ctgatcgacc agctctttaa ttccagtgaa agacgcctcg cgcgcctgct tctgctgctg
     5161 gcgaatttcg gcgaggagca cggcaaacag ccgatcgcga tcaccctcag ccaggaaacg
     5221 ctggcggaaa tgatcggcac cacgcgttcc cgggtaagtt ttttcatgaa caaattccgc
     5281 aaacagggct acatcgacta caacggcaag atcgaggttc accgatcgct gctggatgtt
     5341 gttttgcggg acaaacctca aatacaacag gacgagtagc cggcgctcgc cgttcgcatt
     5401 ccatcagcgg aagcgagcaa tattgctcac taagcactcc accgggtaga cgggaggctt
     5461 ggacacgatc aaattttggg catgccgcag tatcgatttc aggtgcgtcg gggcaagttt
     5521 tccaacggat caagcgttga ggctaccctt gaagatagcg aagctgcctg gaaagaggcg
     5581 gccgcgatct gcgccgatct ggcccgcgac atcatcaggg ccgaccccga atggctcctg
     5641 gaagtgacgg atgaggccgg gcaggggctg ttcaatttcc ggtttgccgc ggagtcgctc
     5701 caaccgaagg ttggagcgga cttacatgcc gttttgacac attagcgctg atcggttgcc
     5761 ctcatcgtcg atgctagccc tcaattagac agttccccgc ttcatttcgg aaccaatccc
     5821 gaaacgccgg cgcattggca gcaggaagtg gacagcggct catatcagcc agctggttgc
     5881 gctcatcgac gcaggctgtt cggccgcatc tgctgccatt gccctgaaac gctcgatatc
     5941 agtggttcaa aacaaggcgc ggcgcctcgg caaaccgttt cagatggccg cctcgcgtcg
     6001 gtgatcttcc gcggccgggc gacgacagaa caaacgaagt gatgcgatgc aaacgctttt
     6061 cttgatatgc aagatggcgt tgcgtcggat ggcttcacgg gccaaccagg gagtcgattc
     6121 ggatacccat taaaactggc catttcgatt gtattcgtgg gtttgcctca acggcgtga
//
LOCUS       DASWGM010000448         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1841591,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000448 DASWGM010000000
VERSION     DASWGM010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841591"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGA15_12985"
     CDS             <1..415
                     /locus_tag="VGA15_12985"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX9588641.1"
                     /translation="SHAWSDGGMGMVASDGFAEFLREQLAPLGRVTMRRMFGKTGVFC
                     DGVMLGMVTENTLYFRVDDDNRAAFKEAESFPPLNYEKKGSTIDLSFWRVPERLFDEP
                     DELVTWARAALAAARRVAAKRERTAPTRKSKPQPT"
     gene            754..1803
                     /locus_tag="VGA15_12990"
     CDS             754..1803
                     /locus_tag="VGA15_12990"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX9588642.1"
                     /translation="MKNTRDAGGLARLISSHERMSLGHFPTALEPLKRLGKWLGGPSV
                     WVKRDDASGMGQGGNKVRALEFVLPEALAAGADILLTSGVVQSNSVRQVAAAAAKAGL
                     DCHFAMITDRVDRTDSDYAQTGNIFLDHLYGATHEIISVKDDRTVVLERIAARMRAEG
                     RRPYIVPYGCANRLGGIGYLNAAAEIATQAKALGVTVTHIVHASGTGGTQAGLIAGFA
                     ALGLPIDVIGIDIDADPAGVRNRVTHILRELADEVDLEPGPLEDKIIIETSFSAGAYG
                     RADERTVDAMAIAARLEALTVDPVYSGKGLAGLIGLSKAGRFKPSDNVVFLHTGGTPA
                     IYAYRSLFGYGSRDD"
     gene            1853..2290
                     /locus_tag="VGA15_12995"
     CDS             1853..2290
                     /locus_tag="VGA15_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588643.1"
                     /translation="MQGVAALLGIAGGLAALSGSPPTYLWICLALVSLTAALALRSVR
                     LVVTSDSVILRLPGLARTFPVDQIDPIRCNELAFMDLFQISLHNGRFMLIPRKAFSDV
                     APFAAMDALISKSAPIRSEQNDIDAVVADRSGPSAAGPRRETP"
     gene            2404..3027
                     /locus_tag="VGA15_13000"
     CDS             2404..3027
                     /locus_tag="VGA15_13000"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9588644.1"
                     /translation="MTASAFATQRLREAILNGELGPGDRLQQHFLAAKLGLSHVPLRE
                     ALQRLEFEGFIAISPRRGAFVVPLTAADAAEIFELRVNLETTALRASVKHLSPEQRLL
                     ATEICRHADGVKDKLAYGEVNWQFHRALYAACQRPRTLNLIETLWRNASRYSMLLRHR
                     APYLRQSQQEHWDILEAAGKQDPELACRLLRAHIEGAAGRVLNLLQP"
     gene            3717..3953
                     /locus_tag="VGA15_13005"
     CDS             3717..3953
                     /locus_tag="VGA15_13005"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEX9588645.1"
                     /translation="MKTKKGAKNERPSALDVVALLADLPAQRLARGQVGTVVEQLDDK
                     TLLVEFSDDQGRAYAVAPCQRADLLILHYVPEAA"
BASE COUNT          775 a         1238 c         1244 g          729 t
ORIGIN      
        1 atcacatgca tggagcgacg gagggatggg gatggttgca agcgacggct ttgccgagtt
       61 cctgcgcgaa cagctcgctc cgctcggccg cgtcaccatg cggcgtatgt tcggcaagac
      121 cggcgtgttc tgcgacggcg taatgcttgg gatggtgacg gagaacacgc tctacttccg
      181 ggttgatgac gacaaccggg cggccttcaa agaagccgag tcctttccgc ctctcaacta
      241 cgagaagaag ggctccacca tagacctctc cttttggcgg gtgccggagc ggctgttcga
      301 cgaacccgat gagcttgtca catgggcgcg cgcagcgctg gcggcggcgc gccgcgttgc
      361 ggcgaagcga gaacggacgg cgccaacgcg aaaatcaaag cctcagccaa cgtgaagagc
      421 atccgcgcgc cggcggccgc ggacataacg accaatttat cgcgcacagg gcccagggcg
      481 ccgccgcaac agcgcgtaca agggcccgag tagtcccagg tgcttgagca caaccagaat
      541 aaccaggcca gccacggcgg cagccgacag agccagatgc gaccatagca tgccgtaaat
      601 tatatattat aatatttttc aatatagatt ataatctttg cgcggccacc gaagcagtcg
      661 gcgtgcgggc gatgccgcgt tcagtttccc cgctgcgcga gcggccgaga agccgccttg
      721 cgccagtaat attttgatca aagagaaggc gagatgaaaa atacgagaga cgccggcggg
      781 cttgcgcggc tgatttcttc ccatgagcgt atgagcctcg ggcactttcc taccgctctg
      841 gagccgctga agcgacttgg caaatggctt ggaggaccgt ccgtctgggt gaagcgggat
      901 gacgccagtg gcatgggcca gggcggcaac aaggtgcgcg ccctcgaatt cgtgcttccc
      961 gaggcgttgg cggccggcgc cgacattctg ctgacctcag gagtcgtcca gtcaaattcc
     1021 gttcgccaag tcgccgcggc cgcggcgaag gcaggcctcg actgccattt cgcgatgatc
     1081 accgaccggg tggaccgcac cgattcggac tacgcacaga caggaaacat cttcctcgat
     1141 cacctctacg gcgcgaccca cgagatcatc agtgtcaagg atgaccgcac cgtggtgctg
     1201 gagcggatcg cggcgcgcat gcgcgcggaa ggccgcaggc cctacatcgt tccctacggt
     1261 tgcgccaacc ggctcggtgg catcggctac ctcaatgccg ccgccgagat cgcgacgcag
     1321 gccaaagcac tcggcgtgac cgtgacccac attgtccacg cgagcgggac cggcggcacc
     1381 caggccggcc tcatagctgg tttcgccgcg ctcggcctgc cgatcgatgt catcggcatt
     1441 gacatcgacg ccgatcccgc cggcgtcaga aaccgagtga cgcacatctt gcgcgaactg
     1501 gcggacgaag tggacctcga gccgggaccg ctcgaagaca agatcatcat tgaaaccagc
     1561 ttctcagccg gcgcatacgg tcgggcagac gagcggacgg tagatgcgat ggccatcgcg
     1621 gcgcggctgg aggccctgac ggtcgacccg gtctattcgg gtaagggcct cgccgggctc
     1681 atcggcctgt cgaaagcggg ccgcttcaag ccgtctgaca acgtcgtgtt cctgcatacc
     1741 ggcgggacgc cggcgatcta cgcctaccgg tcgctattcg gatacggcag ccgcgatgat
     1801 tgacgattcc gccgccaagg aatttcggcc tgacagtcac ggctttctgg tcgtgcaagg
     1861 cgtcgccgcc ctccttggca tcgcgggcgg actggcggcg ttgtccggtt ctccacccac
     1921 atatctatgg atatgcctgg ctcttgtttc gctgacagcg gcactcgctc tacgctcggt
     1981 gcggctcgtc gtaacgtcgg atagcgtcat tctcaggctg cccggccttg ctcgaacctt
     2041 tccggtcgat cagattgacc caatccgttg caacgagctg gcattcatgg atctattcca
     2101 gatctccctg cacaatggcc gcttcatgct gattccacgc aaggcgttct ccgatgtggc
     2161 gccttttgct gctatggatg ccttgatctc gaaatcagcg ccaattcgct cggaacagaa
     2221 cgatattgat gcggtggtcg ccgatcggtc gggaccatcc gcagcagggc ctcgcaggga
     2281 aacgccttga tgaaagacat gcggcaggca aagccagcga agcggaaaac gcaatcggag
     2341 cagccggcga cagctgcgcg tatcgcaggt cgaccaacag gtgaagccag cgaaccgtcg
     2401 cacatgaccg cgagcgcgtt tgcgacgcag cggcttcgcg aggccatcct gaacggcgag
     2461 ctggggccgg gcgaccggct ccagcagcat ttcctcgccg cgaagctcgg gttgagccac
     2521 gtcccgctcc gggaggcgct tcagcgcctg gaattcgagg gcttcatcgc catcagtccg
     2581 cgccgcggcg ccttcgtcgt gccgctcacg gccgccgatg cggctgagat tttcgaactt
     2641 cgcgtcaatc ttgaaaccac cgctctgcgc gcctccgtaa agcatctctc gccggaacaa
     2701 cgtcttctcg cgaccgagat ttgtcggcac gctgacgggg tgaaggacaa gctcgcttac
     2761 ggcgaggtaa actggcaatt ccatcgtgcg ctctatgccg cttgccagcg tcctcgcacg
     2821 ttgaacttga tcgagactct gtggcgcaat gccagccgct attcaatgct gctgcggcac
     2881 cgcgctccct acctccggca atcccaacaa gagcattggg atatcctgga ggcggccgga
     2941 aagcaggacc ctgagctggc ctgccgtctg ctgcgtgcac acatcgaggg cgctgctggc
     3001 cgagtcttga atttgctcca gccctgatgc cttctgcgaa ggaccggggt ggcttgaaag
     3061 tacaaccctt gactccaagg ctttttcgca agcttgcggt gctgatccgc cgtgacaagc
     3121 ggctgcgtcg cggtctgcat tgcagatcgt agctggtgca aggccgcacc cgggcgatgc
     3181 gagcgcgtgc ggccatggcg tggaaaatcg taaagtggcg cgccatggtc gatgaagatg
     3241 ggcaatattg cttcgccgtg gcgctatgat cgtctagccg gggtcgcgcc tggatatcca
     3301 aatattcgct gcccttctga ggggcagtat cgctgatccg cttgcgccat accgggcgcg
     3361 aagccaatct gctgttatga cgaagcatct gccacacagc gagcaggcta tcctcgatat
     3421 ccgcaagatc gagggctatt gcctgaaccc atcgcacccg cgaggccgac acacaaggcc
     3481 cgggtattcc gcgaggccct tgatcttcag cggagcgatg catcgtggct atgcgatgct
     3541 ctactcgaag ccgcacgctc cagcgaggcc tccccgatcg cggtgaaccc ctgggggaca
     3601 cattggcgcc tcgatgcgac catcaggcga caaggaaata gtgctgtggt aagaacgatc
     3661 tggatcgtgc gaactggcga gagcgtgcca aggttcgtca cctgttgggt gctgtgatga
     3721 agacgaaaaa gggcgcaaag aacgaacggc cgtccgcttt ggacgtggtg gcattgctgg
     3781 cggaccttcc cgcgcagcgg cttgcacgcg ggcaggtcgg cactgtcgtc gagcagcttg
     3841 atgacaagac gttgctcgtg gaattcagcg acgaccaagg tcgcgcctac gcggtcgcgc
     3901 cctgccagcg agcggacctg ctcatcctgc actacgtccc ggaggcggcc tgaccgacga
     3961 acgacggcgt agagacggac cctcgc
//
LOCUS       DASWGM010000449         5813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_50662,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000449 DASWGM010000000
VERSION     DASWGM010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50662"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VGA15_13010"
     CDS             complement(<1..720)
                     /locus_tag="VGA15_13010"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HEX9588646.1"
                     /translation="MEISRMDDRTHRAAHRIEGRSAAPGVALGPLVRLAPAKHEARQY
                     RSAAEERQALVDALAAAQADLTALVREVRDDEAEAILSFQIALLEDDNLAAPAFALIA
                     GGEVANRAWSAALALQIASYDGASDLYFRARASDLRDLRDRVLRHLAGEVDQIVPPGV
                     IVAADDMPPSMFLATDWRDGGLVLRRGSPSSHVAILARSRGVPMIVGVDVDHLENGTD
                     ALLDGEAGLLIIDPDTDTRAVY"
     gene            complement(695..949)
                     /locus_tag="VGA15_13015"
     CDS             complement(695..949)
                     /locus_tag="VGA15_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX9588647.1"
                     /translation="MRHEVGLHARPSVKLTKLAKAFDSRIDLGLSPDGPWVDAKSIVK
                     VMATKAPKDSTIYFRAEGTDAKAALEALVTLVDGDFQDGR"
     gene            complement(993..1385)
                     /gene="dhaM"
                     /locus_tag="VGA15_13020"
     CDS             complement(993..1385)
                     /gene="dhaM"
                     /locus_tag="VGA15_13020"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEX9588648.1"
                     /translation="MSQNVGIVIVSHSADVARGTALMVEQMVGREVPLGWCGGDPGGG
                     LGTSVEAIMRAIEQAWSERGVAILVDLGGAETNSEMAIEMLPEERRDRVVVCNAPVVE
                     GAVMAATEAASGATLEAVRRTAEELTPV"
     gene            complement(1382..1996)
                     /gene="dhaL"
                     /locus_tag="VGA15_13025"
     CDS             complement(1382..1996)
                     /gene="dhaL"
                     /locus_tag="VGA15_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEX9588649.1"
                     /translation="MMIDRQARKLLFETVAHRVIASADELTDLDRAIGDGDHGINMRR
                     GFEAVLAAVDELSAKNLGESLKGVGTTLVMRVGGASGPLYGTLFMSLGKSLEDEVTCE
                     RAADAFAAAIEAVKARGKSDVGQKTMLDVLFPVLEVLREGGADMAARLKAAAKVAAEG
                     TIPMRAIRGRASFLGERSIGHMDPGARSSALIVDAVADVMEGFA"
     gene            complement(1993..3030)
                     /locus_tag="VGA15_13030"
     CDS             complement(1993..3030)
                     /locus_tag="VGA15_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588650.1"
                     /translation="MAEVALVDVSRSYGAVEAVRGLSLSISDGEFLVLLGPTGAGKTT
                     TLRLIAGLERPDRGNVLIQGRVVTDEVPAERDVAFVFQQYSLYPHLTVYDNLAFPLRS
                     PARRMNEKAVRRRVQQVSELLHIEQKLGNRATALSGGEMQRVAIGRALVREPSVYLMD
                     EPLSSLDAKLRAELRLELKRIQRELGTTILYVTHDQIEAMTMADRIGVMNDGVLIQLG
                     TPREIYERPDSAYVASRLGTPAINLIPAELLGCDTMPPQARTVGLRTEHIRISSADRP
                     GPLARVHWVEHLGDQNHVHLKLADHSLVTLADPVQPLKAGDQVSLEFAEPLYFDQAGN
                     RIGADGFRGHA"
     gene            complement(3023..4120)
                     /locus_tag="VGA15_13035"
     CDS             complement(3023..4120)
                     /locus_tag="VGA15_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588651.1"
                     /translation="MAEIKVEAVAKTFGTFAAVKGVTFTVDHGSFFVILGPSGCGKTT
                     TLRMIAGLELPTSGRILLDGEDVTALRASARDIAFVFQLFALYPHMNVRQNIGFPLRC
                     QNYPRAEIKQSVEEAARLLRIDHLLERRVGGLSGGDRQRVALGRAIVRRPKAFLMDEP
                     LGALDSEFRRLMCGELRELHDRIDATTVYITHDQLEAMAMADRIAVMNQGRVEQIGTP
                     QEIYDRPRTMFVADFIGAPPMNFLGVQASLQRGDRRIEIDGVRIEMPEVFEDRAEGSF
                     ALGVRPENVSFADASPLRGRVIGAEYLGTTQIVTVITASGQVKARLPSNKAVQVGETV
                     GLALRPDRLSLFDAISGQALRSSLYEGGTRG"
     gene            complement(4126..4329)
                     /locus_tag="VGA15_13040"
     CDS             complement(4126..4329)
                     /locus_tag="VGA15_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588652.1"
                     /translation="MTGKIARYASRWPFWLRRARCEAAAMALIGLGVLMLMQPLSLNL
                     YSYSFVTILAGTLGFVIVSHFPD"
     gene            complement(4326..5279)
                     /locus_tag="VGA15_13045"
     CDS             complement(4326..5279)
                     /locus_tag="VGA15_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9588653.1"
                     /translation="MKMPVTAHSVAEPSRWSKTSASFIVATYAIVAILPLLWIFLTGF
                     KTPPDSIAYPPKILFEPSVEGYVNLFTTRSRQTPEFLASLPPPQTWYERLVRSRNMVI
                     GGPSKVVPRFVNSMVIGFGSTFLAVCFGTMAAYAFSRFRVPLADDLLFFILSTRMMPP
                     IAVAIPIYLMFRQLGLTDTKLGMILLYTAVNVSLAVWLLKGFMDEIPREYEEAAMVDG
                     YTRLQAFFKVVLPQATTGIAATAIFCLIFAWNEYAFAVLLTSGDAQTMPPFIPFIIGE
                     GGQDWPAVAAATTLFFIPILCFTILLRRHLLRGITFGAVRK"
     gene            complement(5276..>5813)
                     /locus_tag="VGA15_13050"
     CDS             complement(5276..>5813)
                     /locus_tag="VGA15_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9588654.1"
                     /translation="AVVGNFWTFLLQPQVGLFNSAIALLTGIDPSSFQMIGDIRLAPW
                     SIIMVDTWMWTPYVMLICLAGLRSIPDYIYEAAEVDRASPLRQFWSITLPMVMPFLML
                     AILFRAIENFKMFDMVNLLTSGGPGSTTEVASITLKREAFEKWRTGYSSALAIILFVT
                     VFGAANIYVKALNRVKQR"
BASE COUNT         1259 a         1802 c         1687 g         1065 t
ORIGIN      
        1 ataaacggct cgcgtatctg tatcaggatc gatgatgaga agccctgcct ccccatcgag
       61 cagcgcgtcc gtgccgtttt cgagatgatc cacgtctact cccacaatca tcggcacgcc
      121 gcgcgatctt gcgagaattg ccacatggct gctggggctt ccccggcgca ataccaggcc
      181 gccgtcacgc caatcggtgg ccagaaacat cgaaggaggc atatcgtcgg ccgcgacgat
      241 aactcccggg ggcacgatct gatcgacttc acctgccaga tgtctcagca cgcgatcacg
      301 cagatcgcgc agatcggatg cgcgagcgcg gaaataaaga tcgctggccc catcgtacga
      361 tgcgatttgc aaggcaagtg ctgcgctcca cgcccgattg gcaacctcgc cgccggcgat
      421 cagcgcaaac gccggcgctg cgagattgtc gtcttcgagc agcgcaatct ggaaagagag
      481 aatggcttcg gcctcatcgt ccctgacttc gcgcacgagc gcggtgagat ctgcttgggc
      541 ggccgcgagt gcatcgacga gggcctggcg ctcctccgcc gcggatcgat attgacgggc
      601 ttcatgcttt gcgggagcca aacgcacgag tggtcccaac gctaccccgg gagcagcgga
      661 tctgccttcg atgcgatgag cggctcgatg cgttctatcg tccatcctgg aaatctccat
      721 cgacgagcgt tacgagcgcc tccagcgcgg ccttagcatc cgtcccctcc gcacgaaaat
      781 agatggtcga gtctttcggc gccttcgtcg ccatcacctt gacgatactc tttgcatcga
      841 cccagggccc gtcgggcgac agcccgagat cgatacgcga atcgaaggcc ttcgcaagct
      901 tggtcaactt gaccgagggg cgcgcatgca acccgacttc atgtcgcatg agcactgatg
      961 cccgaagctt attcattccg cttgcccccg catcagaccg gcgtcagttc ttcggcggtg
     1021 cgacgcacgg cctcaagcgt ggcgcccgac gctgcttcgg tcgcggccat gactgccccc
     1081 tcgaccacag gcgcattgca aacgacgaca cggtcgcggc gctcctcagg caacatttca
     1141 atcgccatct cgctgtttgt ttcggcgccg ccgaggtcga ccagaatggc aacaccgcgt
     1201 tccgaccacg cctgttcgat cgccctcatg atcgcttcga cacttgtacc caatccgccg
     1261 cccggatccc cgccgcacca gccgagcggc acttcacggc cgaccatttg ctcaaccatg
     1321 agagcagtgc cccgcgccac gtccgccgag tgagacacga tgacgattcc aacattctgg
     1381 ctcatgcaaa tccttccatg acgtctgcca ccgcgtcgac tatcagcgcg gacgatcgcg
     1441 cgccgggatc catgtgacca atgctgcgct cgccgagaaa cgaagcgcga ccacggatcg
     1501 cccgcattgg aattgtaccc tctgccgcaa ccttggccgc agccttcagg cgcgccgcca
     1561 tgtcagcccc gccctcgcgc aagacttcca gcacgggaaa aagcacgtcg agcatggtct
     1621 tttggcccac atcggacttt ccccgtgcct tgaccgcttc gatcgcggct gcaaacgcat
     1681 cggcggcccg ctcacaggtg acctcatctt ccaacgattt tccgagcgac atgaacaagg
     1741 tcccgtagag cggaccagaa gcgccgccaa ctctcatgac gagggttgtc cctacgccct
     1801 tcagggactc gccaaggttc tttgcggaga gctcatccac cgcggccagg acagcttcaa
     1861 agccgcggcg catgttgatc ccgtggtcgc cgtcgcctat tgcgcgatcg agatcggtaa
     1921 gttcgtcggc gctggcgatg acacgatgcg ccacggtttc aaacaacagt ttgcgagcct
     1981 gacgatcgat catcatgcat gcccccggaa tccgtcagca ccgatccggt taccggcctg
     2041 atcaaaataa agaggttcgg cgaactcgag agagacctgg tcccctgcct tcagcggctg
     2101 cacagggtcc gcaagggtca cgagcgaatg gtcggcgagc ttgagatgaa cgtggttctg
     2161 atcaccgaga tgttccaccc aatggacccg tgcgagtggt cccggacgat ccgctgaaga
     2221 aattctgata tgctcggtcc gcaagcccac ggttcgcgcc tgcggcggca tcgtatcgca
     2281 ccccagaagc tcggcaggaa taagattgat ggccggtgtg ccgagccgac ttgccacata
     2341 ggcactgtcg ggccgctcat aaatctcccg cggtgtgccg agttgaatga gaaccccatc
     2401 gttcatgacg ccgattcgat ccgccatcgt catggcctcg atctggtcgt gcgtcacgta
     2461 gaggatcgtt gttcccagct cacgctggat acgtttgagc tcaagacgca attccgcacg
     2521 aagcttggca tccagcgagg aaagcggttc gtccatgaga tagacactgg gttcacggac
     2581 cagtgcgcgg ccgatggcga cccgctgcat ttcgccgccg gaaagagcgg tagctcgatt
     2641 accaagcttc tgttcgatat gcagtagttc cgacacctgt tggacccgac ggcgaactgc
     2701 cttttcattc atgcgccgcg ccggagaccg caatggaaag gccaggttgt cgtagactgt
     2761 cagatgagga taaagcgaat attgttgaaa cacgaaggcg acgtcgcgtt cggccggcac
     2821 ctcgtccgtt acaacgcgcc cctggatcaa cacgttgcct cgatcgggcc gctccaggcc
     2881 ggcgatcaaa cgcagcgtcg ttgtctttcc agcaccggtt gggccaagca acacgagaaa
     2941 ttcaccgtcg gagatggaga gcgaaaggcc ccggaccgcc tccacggcgc catagctcct
     3001 gctgacgtcc acaagtgcga cctcagccac gggttcctcc ttcgtaaagc gatgaacgca
     3061 acgcttgtcc gcttattgca tcgaacaacg agagccggtc cggccgcaac gcgagtccca
     3121 ctgtttcacc gacctggacc gctttgttcg atggcagtcg cgccttgacc tggccgctgg
     3181 cagttatcac cgtaacgatt tgcgtcgtac cgagatattc ggcgccaatg acacgacctc
     3241 gcaacggcga ggcatcggcg aacgacacat tctcgggccg cacaccaagg gcgaagctgc
     3301 cttcggcgcg atcctcgaat acctccggca tttcgatgcg gacgccgtca atctctatgc
     3361 ggcgatcgcc acgttgcagt gaggcttgaa cgccgagaaa gttcattggt ggcgcgccga
     3421 taaagtcggc cacgaacatg gtacggggac gatcgtagat ctcttgcggc gtgccaattt
     3481 gctcgacacg gccttgattc atgacggcga tacggtccgc catcgccatg gcctcgagct
     3541 gatcgtgggt gatgtagacg gtggtcgcgt caatccggtc gtgcaactcg cgcagctcgc
     3601 cgcacatcaa tctgcgaaat tcggaatcga gcgcgcccaa aggctcatcc atgaggaagg
     3661 ccttgggccg gcgcacgatg gcccggccca aggcgacgcg ctgccggtcg ccgccggaga
     3721 gtccgccgac ccgacgctcc aaaaggtggt cgattctcag caatcttgcc gcttcctcaa
     3781 cgctttgctt gatctcggcg cgcgggtaat tctggcatcg cagcggaaag ccgatgttct
     3841 ggcgcacgtt catgtgcggg tagagcgcaa atagctgaaa cacaaatgca atgtcgcgcg
     3901 ccgaagcgcg cagcgcagtt acatcttcgc catcaagcag gatgcgaccg gaggtcggca
     3961 gttcgaggcc cgcgatcatg cgcaaagtcg ttgtcttgcc gcaacctgaa ggtcccaaaa
     4021 tgacgaagaa cgagccgtga tcgaccgtga aggtcacgcc tttgaccgcg gcaaaggttc
     4081 cgaatgtctt ggcaacagcc tctaccttga tctctgccat cgcggctaat ccggaaaatg
     4141 actgacgatc acaaacccca acgtgccggc caggatcgtg acgaaggaat aactatacag
     4201 gttcaaagag agcggctgca tcagcatcag aactccgagg ccgatcagcg ccatcgccgc
     4261 ggcttcgcaa cgggcgcgac gcagccagaa cggccatctt gacgcgtaac gcgcgatctt
     4321 ccctgtcatt tgcgcacggc cccaaaggtg atgccgcgca acaagtggcg acgtagcagg
     4381 atcgtgaagc acaggatcgg gatgaagaac aacgtcgtcg cggctgcgac cgcgggccag
     4441 tcctggccgc cctcgccaat gatgaagggt atgaagggcg gcatggtttg cgcgtcgccg
     4501 ctggtgagca ggaccgcgaa ggcatattcg ttccaggcaa agatcaggca gaagatcgcg
     4561 gttgccgcga tgccggtggt cgcctgcgga agaaccacct tgaaaaatgc ctgaaggcgg
     4621 gtgtagccgt cgaccatcgc agcctcttcg tactcgcgcg ggatttcatc catgaatccc
     4681 ttcagcagcc acaccgccag agagacattc acggcggtgt acaacaggat catgccgagc
     4741 ttggtgtcgg ttaatccgag ctgccggaac atcagataga tcgggatcgc aaccgcgatc
     4801 ggcggcatca tccgtgttga caggatgaag aacaagagat cgtcggcaag cggcacgcgg
     4861 aaacgcgaga acgcataggc cgccatggtg ccgaagcaca ctgcaagaaa ggtcgagccg
     4921 aagccgatga ccatcgagtt cacaaaccgc ggcacgacct tcgatggccc gcctatcacc
     4981 atgttgcgag agcggacaag gcgctcatac caggtctgcg gcggcggcaa gctcgccaga
     5041 aactccggcg tctgccgcga gcgcgttgtg aagaggttga cgtagccctc gacggaaggc
     5101 tcgaacagaa ttttgggtgg atacgcaatc gaatccggtg gcgtcttgaa accggtcagg
     5161 aatatccaca acaatggcag gatggctacg atcgcatatg tagcgacgat gaatgaagct
     5221 gaagtcttcg accatcgtga cggctcggcc acagaatgcg cggtgacggg cattttcatc
     5281 gctgcttcac ccgattgagc gctttgacgt agatattggc ggcgccaaag acggtgacga
     5341 agagaatgat ggccaacgcc gatgaatagc ccgtgcgcca tttctcgaac gcctcgcgct
     5401 tcaaagtgat cgatgcaacc tccgtagtcg atcccggacc gcccgaggtg agcaggttga
     5461 ccatgtcgaa catcttgaag ttctcgatgg cccgaaacag aatcgcgagc atcagaaacg
     5521 gcatcaccat cggaagtgtg atggaccaga attgccgcaa gggggatgcg cgatcaacct
     5581 cggcggcctc atagatgtaa tcgggaatcg aacgcaatcc cgcaagacag atcagcatga
     5641 catagggcgt ccacatccag gtatcgacca tgatgatgga ccagggcgcc agccgtatgt
     5701 cgccgatcat ctgaaacgag ctcgggtcga tcccggtaag gagtgcgatc gcactattga
     5761 acaatccgac ctgcggttgg agcagaaagg tccagaagtt gccaacgacc gcc
//
LOCUS       DASWGM010000450         5165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_309116,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000450 DASWGM010000000
VERSION     DASWGM010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309116"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..924)
                     /locus_tag="VGA15_13055"
     CDS             complement(253..924)
                     /locus_tag="VGA15_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9588655.1"
                     /translation="MGKLRVGMIAIGVLGGIADPVAKFIGSKLGVAVEPVMYTSPGAY
                     AESFGKDEWDIAIGPRALAPEEKADSTVDIWTISLVYVAAPGKEFANIAAVDKPGVKI
                     GTIQGAPSDRILTREVKSAEIARIPLSPHIAADAAEMLRAGKADVFGADSGVGYPAVE
                     ALPGSRLVPGVFGTVRVAAALPKGLPTDVRAKLDELIAEAKRTGVVQQAIDTKGLKGV
                     SVAPN"
     gene            complement(1306..1794)
                     /locus_tag="VGA15_13060"
     CDS             complement(1306..1794)
                     /locus_tag="VGA15_13060"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9588656.1"
                     /translation="MLAGGLDDWCYIFASRGNEEHWRRLVKAIGREDLLNDPRMVDGA
                     TRAQNREAVDAAITEWTSKRTKHEVTRIIAGAGVPCGAVLTTLELLHDPDLHKRGMMQ
                     KIEHPVRGPVIVPAWPLRMSDSKVPLKCAPILGADSEAIYGEWLGCSPDEVRDMRQGK
                     VI"
     gene            complement(1716..2549)
                     /locus_tag="VGA15_13065"
     CDS             complement(1716..2549)
                     /locus_tag="VGA15_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9588657.1"
                     /translation="MRERVVQPEIATGIQPALKGVKVLDLTQFEAGPSCTEALAWLGA
                     DVVKVEEPHRGEPGRWGVSDRPDADSHYFIYYNLNKRSVTCNLKSDGGKALLRRMIGK
                     ADVLIENMAPGTFARLGFDYPRLSALNPRLIFAQVKGFAPESEHANYLSFDMIAQAMG
                     GTMGVNGHPDEPPVRPGPTIGDTGTGMLCAMGIIAALYQRMTTGRGQHIQIAMRDAML
                     NYCRTPMSRQAARDDQLPRGGNTVVGTAPGDLYKCSPGGSTTGATSLPRGATRSIGAA
                     W"
     gene            complement(2612..4270)
                     /locus_tag="VGA15_13070"
     CDS             complement(2612..4270)
                     /locus_tag="VGA15_13070"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9588658.1"
                     /translation="MSEVDGATLIARSLKQQGIDHLFGVVGFPVTPIAAAAQKEGVAY
                     LGMRNEQSASYAAQAYGYLTGRPGACIVVTGPGVVHGLAGLANAQQNCWPMILIGGAS
                     ETYRGGMGAFQEERQVLIASPFCKFAHGIESVQRIPYYVEMATRHAIYGRPGATYLDA
                     PDDIINGKCDLDEVVEVERVPEPPRMVAPTENVAAALNLLERAERPLVLLGKGMAWSR
                     AEDEVRAFIERTQLPFLRSPMGKGVMPDDHPLSAAAARSLALQQADVIFLMGARLNWI
                     FHFGQAPRYAKDVKIIQLDIAPEEIGHNKATEVALVGDGKAIVGQLNKALDNRQWFHP
                     NDTPWRQAIAKKTADNAAQIRPQIDDDQAPAGYYRALRDVAAWMPRDAILSAEGASTM
                     DIGLTQLPSFNARSCLNAGTYGTMGVGLGQAIAACVAHPDRPVIHLSGDSAIGFSGME
                     METLVRYNLPAKIVVLNNGGIGPGMPQIPENPMFNLKPNALIWGARYDRVMEAFGGKG
                     FLVEDPKDLRGALDEAMNFRGPALVNVVISQGSARKAQEFGWHS"
     gene            complement(4456..5022)
                     /locus_tag="VGA15_13075"
     CDS             complement(4456..5022)
                     /locus_tag="VGA15_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9588659.1"
                     /translation="MPPLVQSLRKYYGLIRLLQIEGRTRDLAMFNLAIDSKLRGCDVV
                     AIRVEDVAAGGYTADRATVRQKKTGRPVRFELSEQTRKAVDDYLKTANKRPGEFLFTG
                     RRGPNRNMTTRQYARLVSEWIGSVGLDPRLFGTHSLRRTKATLIYRRTGNLRAVELLL
                     GHTKIESTVRYLGIEVDDALAIAEQVDV"
BASE COUNT          902 a         1655 c         1630 g          978 t
ORIGIN      
        1 aacgcccagc caacacgagt cgaatcattt gatcgaagcg cgttagcgta acttctcgaa
       61 ccgccgtata cggacccgta cgtacggtgg tgtgggaggg gcggagccgc gaggctcccc
      121 cctatcccga tctctggcac gaaccggacc aacctggccg gtctgacgat gtccgttgat
      181 cggggtagac cggaagtggc aggcagacgc gttccgactg gccacacccg tatcgtccgc
      241 cgacttgatt tttcaattgg gcgcgacgct gactcccttg agccccttcg tgtcgattgc
      301 ctgctggacg acacctgtgc gcttggcctc cgcaatcagc tcatcgagtt tagcgcggac
      361 atctgtcggt aggcccttcg ggagcgcagc cgccacgcga accgttccga acacgcccgg
      421 taccaaccgt gagccaggca gagcttcgac agcaggataa cccacgcctg agtccgcgcc
      481 gaacacgtcg gccttgccgg cgcgcagcat ctcggctgca tcagcagcaa tatgtgggct
      541 caatgggatg cgcgcgatct cggccgattt gacttctcga gtaagtatcc ggtccgatgg
      601 ggcgccctgg atcgtcccga tcttaacgcc cggcttgtcg acggccgcaa tattcgcaaa
      661 ttcttttcct ggcgcagcga cgtagacgag actgatggtc cagatgtcga cggttgaatc
      721 agccttttct tccggcgcta aagcgcgcgg accgatggcg atgtcccatt catcctttcc
      781 gaaactctct gcatatgctc ctgggctggt atacatcacc ggctcgaccg caacacccag
      841 cttggaaccg atgaatttag cgactgggtc ggcaatgcct ccgaggaccc cgattgcaat
      901 catgccgacg cgcagctttc ccataggaac aatctcccgg gatacgttcg gctgaccgaa
      961 cgccgttgat atcatcgata tcgacaatcc gatggacagt aatatccggc gcataaaatc
     1021 ctccgcctcg aaaaaccccg agcgtgtccg ctagtagcaa ctttaggatt atatcgtttc
     1081 acgaaggcga gttctgagaa tacttggtca cggctggggt ccgctcatgg cggcacaaaa
     1141 cggacctccg ccgatgtctg caattcggtc gctatcgggg gtaaaccgga catgacgcgg
     1201 acggcccagt ttggtcgcga atgacccgga catccgaagg cgcttagcat tcatgcgcga
     1261 tgcccgcggc ggcagcggct cctcgcgcgg ccaggattgc ggtcctcaga tcaccttgcc
     1321 ttgccgcatg tccctgacct cgtcggggga gcagccgagc cactcgccat agatcgcctc
     1381 ggaatccgcg ccgagaatcg gggcgcattt cagcgggacc ttgctgtccg acatccgcag
     1441 cggccaagcc ggcacgatga ccggcccgcg caccggatgc tcgatcttct gcatcatccc
     1501 gcgcttatgc agatccgggt cgtgcagtag ctcgagcgtg gtgaggacgg cgccgcaggg
     1561 aacaccggcg ccggcgatga tccgggttac ctcatgctta gtgcgcttcg acgtccattc
     1621 agtgatggcc gcatccacgg cctcccggtt ctgggcgcgg gtggcgccgt cgaccatacg
     1681 cggatcgttg agcaggtcct cgcggccgat cgccttcacc aggcggcgcc aatgctcctc
     1741 gttgccccgc gaggcaaaga tgtagcacca gtcgtcgagc cccccggcga gcatttgtag
     1801 aggtcgcccg gcgcggtccc gaccaccgtg ttgccgccac gcggcagctg gtcgtcgcgg
     1861 gcggcctggc ggctcatcgg cgtgcggcag tagttcagca tcgcgtcgcg catggcgatc
     1921 tggatgtgct ggccgcggcc ggttgtcatg cgctggtaga gcgcggcgat gatgcccatg
     1981 gcgcaaagca tgccggtgcc ggtatcgccg atggtcgggc ccggccggac cggtggctcg
     2041 tcgggatggc cgttcacccc cattgtgccg cccatcgcct gggcgatcat atcgaaggac
     2101 agatagttcg cgtgctcgct ctccggcgcg aagcccttaa cctgggcgaa gatcaatcgc
     2161 ggattgagcg cgctcagccg cggatagtcg aagcccaggc gtgcgaaggt gcccggcgcc
     2221 atgttctcga tcaggacatc ggccttgccg atcatccgcc tcagcagcgc cttgcccccg
     2281 tcgctcttga gattgcaggt aacgctgcgc ttgttgaggt tgtagtagat gaaatagtga
     2341 gaatcggcgt cggggcgatc gctgactccc cagcgccccg gctcgccgcg atggggctcc
     2401 tccaccttga ctacgtcagc cccaagccag gccagcgcct cggtgcagga cggtcccgcc
     2461 tcgaactggg tgaggtcgag caccttgacc cccttgagcg ccggctggat cccggtcgcg
     2521 atctctggct gcactacccg ttcccgcatg gcgacccctc ccattaagga gccgcgccgg
     2581 gaatggcgcg gccctttgat gtgtcactcg atcagctatg ccagccgaac tcctgcgcct
     2641 tgcgggcgga gccctgcgag atcacgacgt taaccagggc gggaccgcgg aagttcatcg
     2701 cctcgtcgag ggcaccgcgc aggtccttcg ggtcctcgac caggaatccc ttgccgccga
     2761 aggcctccat cacccggtca tagcgcgcgc cccaaatcag cgcgttgggc ttcaggttga
     2821 acatcgggtt ttccgggatc tgcggcatgc cggggccgat gccgccattg ttcaagacga
     2881 cgatcttggc cgggaggttg tagcggacca gcgtctccat ctccatgccg gaaaagccga
     2941 tcgcggagtc gcccgagagg tggatgaccg gccggtcggg atgcgcaacg caggcggcga
     3001 tggcctggcc gaggccgacc cccatcgtgc cgtaggtgcc ggcgttgagg caggaacggg
     3061 cgttgaagct cggcaattgc gtgaggccga tatccatggt gctggcaccc tcggcgctaa
     3121 ggatcgcgtc ccgcggcatc caggcggcga cgtcgcgcaa cgcccggtag tagccggccg
     3181 gcgcctgatc gtcatcgatc tgcggccgga tctgcgccgc attatcggcc gtctttttgg
     3241 caatcgcctg ccgccacggc gtgtcattgg gatggaacca ctggcggttg tcgagcgcct
     3301 tgttgagctg cccgacgatg gccttaccgt caccgaccag cgccacttcc gtcgccttgt
     3361 tgtggccgat ctcctcgggc gcgatgtcga gctggatgat cttgacgtcc ttcgcatagc
     3421 gcggcgcttg accgaaatga aagatccagt tcagccgcgc gcccatcagg aagatgacgt
     3481 cggcctgctg cagcgccagc gagcgcgccg ccgcggccga cagcggatgg tcgtccggca
     3541 tcaccccctt gcccatcggc gagcgcagaa acggcagttg cgtgcgctca atgaaggcgc
     3601 ggacttcgtc ctcggcgcgc gaccatgcca tgcccttgcc gaggagaacg agcggccgct
     3661 cggcccgttc gagcaggttc agcgccgccg cgacgttctc cgtcggcgcc accatgcgcg
     3721 gcggttcggg cacgcgctcg acctcaacga cttcgtcgag gtcgcatttg ccattgatga
     3781 tgtcgtcggg cgcgtcgaga taggtggcgc cggggcgacc atagatcgcg tgccgggttg
     3841 ccatctcgac atagtagggg atgcgctgca cgctctcgat gccgtgcgcg aacttgcaga
     3901 acggcgaggc gatcagcacc tgccgctctt cctggaacgc gcccatgccg ccgcgatagg
     3961 tctccgaggc accgccgatc aagatcatgg gccagcaatt ctgctgggca ttggcgagac
     4021 ccgcgagacc gtgcaccacg cctgggccgg tcacgacgat gcaggcgccg gggcggccgg
     4081 tgagatagcc gtaggcctgc gccgcgtagg aggcgctctg ttcgttgcgc atgccgagat
     4141 aggcgacgcc ttccttctgc gcggccgcgg cgatcggggt gactggaaac ccgacgaccc
     4201 cgaacaggtg atcgatgccc tgctgtttga ggctcctcgc gatcagggta gcgccatcga
     4261 cttcgctcat ggtttccccc ctcgtgaccg attgaacttc agccgcgaga ttatgcatag
     4321 atggcagggt cgcgaaaggg ccctactttc cgatgtgcga tacagccgag ccatgcggct
     4381 ccgtgcgttc gggcgctatt ggcacaaacc ggcggttgga ggtcgggcag agcatgtccg
     4441 ctctgcccgg gtatttcaga catcaacttg ttccgctatt gcaagggcat catctacttc
     4501 gatcccgaga tatctgaccg tgctttcgat ctttgtatgt ccgagcagaa gctcgacggc
     4561 cctgagattg ccagtccgcc ggtagatcaa ggtagccttt gtgcgtcgca gtgaatgcgt
     4621 cccgaacagc cttggatcca gtccaacgct gccgatccac tcggaaacga gccgcgcata
     4681 ttggcgcgtt gtcatgttgc ggttgggacc acgacggccc gtgaacaaga actcaccggg
     4741 ccgtttgttg gcggtcttta gatagtcatc gaccgccttg cgggtctgct cgctcaattc
     4801 aaatctgact ggccgccccg ttttcttttg ccggaccgtt gcgcgatccg ccgtgtaccc
     4861 gccggcggcg acatcctcga cccggatggc gacgacatca cagccgcgaa gcttgctgtc
     4921 gatcgccaga ttgaacatgg cgaggtcgcg agtgcggcct tcgatctgga gaagtcggat
     4981 gaggccatag tacttgcgaa gcgactgaac aaggggaggc aacgcccggc ggaagtcgtg
     5041 gagggaaggg cctcacccaa ggggaacagc cgttaggcgg ccgcggttcg gacactgagc
     5101 cgaaataccg cgtcgatccg aataatggct gcgcgtcggg cgacgaacgt gactaaatcg
     5161 ttcat
//
LOCUS       DASWGM010000451         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_309196,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000451 DASWGM010000000
VERSION     DASWGM010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309196"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VGA15_13080"
     CDS             complement(<1..941)
                     /locus_tag="VGA15_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9588660.1"
                     /translation="MRFLVRFIGFLFAAGTVVFLVGVAAAAGLIWHFSKDLPDYSQLQ
                     DYEPPVMTRVHASDGALLGEYSKERRLYLPIQAVPKLVINAFLAAEDKNFYEHGGIDF
                     TGMARAAVLFAQNYGSNRRPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRLERT
                     YSKDKILELYLNEIYLGLGAYGIAAASLVYFDKSVNELTVGEAAYLAALPKAPSSLHP
                     VKNRDRAIERRNYVIDRLLENGWVKQADADKARKDPLIVTNRSNAAHTFAGEYFAEEV
                     RRDVFERYGEKKLYEGGLSVRTTLDPKLQVMARKTMAA"
     gene            complement(1160..2461)
                     /locus_tag="VGA15_13085"
     CDS             complement(1160..2461)
                     /locus_tag="VGA15_13085"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9588661.1"
                     /translation="MASRANHRFLLRSALLCAAALLCSETASVGAAEGQPSNPTAASS
                     FPIASDARLAGDAKQTRFVLDLDKPIQFRAFTLADPYRVVMDIPQVDFRLPAEAGTTG
                     RGLIQAFRYGLVMPGGSRIVFDLTGPARIAKSYVLDAANGQPPRLVLELEQVDRTGFV
                     QSLAAESRPELRPAIAVAADPVVATKAAVPEPAAPAVPDSRPVVVIDPGHGGVDNGTQ
                     AGGENEKDLVLGFGLALRDRIEKAGKYRVVMTREDDTFIPLADRVTIARNQSAALFVS
                     IHADALPAGEGDAQGATIYTLSDKASDAEAERLAEAENRADAIGGVKLTEEPTEVADI
                     LIDLVRRETRTFSNRFARQLMNEMKTTVRMHKRPLKSAGFKVLKGPDVPSVLVELGYA
                     SNKDDLEHMKSENWRARTVGSMAQAIDVFFAKRVAKAGPTN"
BASE COUNT          526 a          861 c          819 g          538 t
ORIGIN      
        1 gccgccatgg tcttgcgcgc catcacctgc agcttcggat ccagcgtggt ccgtaccgac
       61 aggccgcctt catagagttt cttttcgccg taccgctcga acacatcgcg gcggacttcc
      121 tcggcgaaat attcgccggc gaaagtgtgc gcggcattac tccggttggt cacgatcagc
      181 ggatccttgc gggccttgtc ggcatcggcc tgcttgaccc agccattttc gagaagccgg
      241 tcgatcacgt aattgcggcg ttcaatggcg cggtcacggt tcttcaccgg atggagcgat
      301 gacggagcct tgggcagcgc agccagatag gccgcctccc cgacggtaag ttcattcacc
      361 gatttgtcga aatagaccag cgacgccgcg gcgatgccgt aggcgccgag gccgagataa
      421 atttcattga gatagagttc gagaatcttg tcctttgagt aggtgcgctc gaggcgcatc
      481 gccagcaagg cttccttgat cttgcgggtg aacgaaacct cattggtcag aagaaagttt
      541 ttcgcgacct gctgggtgat cgtggaagcg ccctgcggcc ggcggttcga gccataattc
      601 tgcgcaaaga gcaccgcggc gcgcgccatg ccggtaaagt cgatgccacc atgctcgtag
      661 aagttcttgt cctcggccgc caggaacgcg ttgatgacca gtttcggaac cgcctggatc
      721 ggcagataca gccggcgttc cttggaatat tcgccgagca gtgcgccgtc cgaggcatgg
      781 acacgcgtca tcaccggcgg ctcataatcc tgaagctgcg aatagtccgg caaatccttg
      841 gagaaatgcc agatcagccc cgccgccgcg gccacgccca ccagaaacac aacggttcct
      901 gcggcgaaca agaaacctat gaaccgcacc agaaagcgca tgatagaaag ttccgtttca
      961 gccccgcgtc atcataaccg gcgccatccc cagcgtgaag agtgcttcgg atcgatttgc
     1021 gattccgcga atgcgcacaa tacattggcc gcgatggtct tttgatcgat tcccaaagac
     1081 tgcgcggtag ctgttttatt ttttataagg ccgccgctgt ggccaaacta gggctttcca
     1141 caatctgacc gctccgtctt caattcgtcg gccctgcctt cgcgacgcgt ttggcgaaga
     1201 aaacgtcaat cgcctgcgcc atcgatccaa cggtccgggc acgccagttc tccgatttca
     1261 tgtgttcgag atcatccttg ttcgaggcat agccaagctc aacaaggacc gacggcacgt
     1321 cggggccctt cagtacttta aacccggcgg atttcagcgg gcgcttgtgc atccgcacgg
     1381 tcgttttcat ctcgttcatc agctggcgcg cgaatcgatt cgagaaggtg cgggtttcgc
     1441 gccgcaccag atcgatcaga atgtcggcca cctcggtagg ttcctcggtg agcttgaccc
     1501 cgccgatggc gtcagccctg ttttccgcct cggccagcct ctcagcctct gcgtcggagg
     1561 ctttatcgga cagggtgtag atcgtggcgc cctgagcgtc gccttcgcca gccggcaacg
     1621 cgtcggcatg aatcgatacg aacagcgccg ccgactgatt gcgggcgatc gtgacccggt
     1681 cggcgagcgg aatgaaggta tcgtcctcac gggtcatgac aacacgatat ttgccggctt
     1741 tctcaatgcg atcgcgcaat gccagtccga atcccaacac caggtccttt tcgttttcgc
     1801 cgccggcctg cgtgccattg tcgacgccgc catggcccgg atcgatcacc accaccggac
     1861 gcgaatccgg caccgccggc gccgcgggtt cgggcaccgc cgccttcgtc gcgacgacgg
     1921 ggtctgcggc gacggcgatg gccggtctca gctctggccg gctttcggcg gcgagtgact
     1981 ggacgaagcc ggtgcgatcg acctgttcca actcaagcac gagccgcggg ggctggccgt
     2041 tggccgcatc cagcacgtag gattttgcga tcctggccgg gccggtcaga tcgaacacga
     2101 tccgcgagcc gcccggcatc accaggccgt agcggaatgc ctggatgagc ccgcgcccgg
     2161 tggtgccggc ttcggccggg aggcgaaagt cgacctgagg aatatccatc accacccgat
     2221 acggatcggc gagcgtaaag gcgcgaaact gtatgggctt atcgaggtca agcacaaacc
     2281 gggtttgctt ggcgtcaccg gccaggcgcg cgtccgaggc gatcggaaag ctcgaggctg
     2341 cggtcggatt cgacggttgc ccctcggccg cgccgacgct ggcggtctcg gagcacagca
     2401 atgctgcagc gcacaatagc gcgcttctca gcaaaaaacg gtgatttgcg cggctcgcca
     2461 acgaatctgt gccctccgag cagccctctt accgcaatag gaccacaggg ttaactgcag
     2521 cttaaaactg ttccccaaat aaggccaagt gttgccgcgc agcgacgctt cccttgcgcg
     2581 cggcggccaa tccacgtatg taaaaggggc tgacggctaa aactcccggt tgtgtcgcgt
     2641 tcagcctccc ggtgcagcgc cggagccttc gaaacttcgg agattccagc gtctttccga
     2701 tccctccgca gccagcgcag cgcgcggctg acaaggagcg gcgg
//
LOCUS       DASWGM010000452         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1277888,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000452 DASWGM010000000
VERSION     DASWGM010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277888"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VGA15_13090"
     CDS             complement(<1..1041)
                     /locus_tag="VGA15_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588662.1"
                     /translation="MFHNNQFTRRDLLKTAGGIALASGISAPAILRAQTADVIRIGHL
                     TPRTGFLGPLGEYAVMAIDLAVEEINASGGMMGRKVELLKEDSVNPQTASTKAERMIE
                     RDKVACIIGEISSASCLTIAQVASRNKTLFINTGGNSDALRGKDCQRYMFHVESQNSM
                     YVKAVGRSFLRDGLVKGKKWYSLTADYAFGHDLLKVSKRFMEANGGNFAGDDLVPTDA
                     TDFSAYLLKIREAKPDLVVINLAGNQITNFLKQYAEFGLTFPVGGFGFDTALAWAAGK
                     GNFVGTWPVVWHHLIDTPGSKTFVAAFTKKYNKPPENQAWGDYTATKIVAQTVNEIKS
                     TESPKIIEHFEKG"
     gene            1218..1916
                     /locus_tag="VGA15_13095"
     CDS             1218..1916
                     /locus_tag="VGA15_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9588663.1"
                     /translation="MNLPAALNLVEPLDRQTLGERAYAKLADLLISGRLAPGEKLSLR
                     AAADVLGVSIMPVREAVSRLVADKALEVTPNRAVRVPLMSAAQFRDLTKVRIAIEGHA
                     AAQAALHRDNTDLGLIARAEEAMKAESQSPAPDLPRAVELNKTFHFAVYEAAHSPILV
                     EIIRALWLKAGPVINLDLRANPERLAKGDAIRFHADVRKAIAAGAGEAALEGIKADIS
                     SAADFILSRGGLAG"
     gene            1947..2723
                     /locus_tag="VGA15_13100"
     CDS             1947..2723
                     /locus_tag="VGA15_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9588664.1"
                     /translation="MDLELKGLRVLVTAGANGIGLAIARAFAGEGARVHTCDVDETAL
                     AALAASDPAITQTSCDVSDRAAVKNLFAETVAKLGGLDVLVNNAGIAGPTSRVEEMNP
                     EDWDRCLEICLTGQFNCTRLAVPLLRQSKNASIVNISSAAGRLGFAMRTPYAAAKWGV
                     IGLTKSLSIELGPDNIRVNAILPGLVAGDRQRRVLEAKAQQRGISYAEMERTAFSYTS
                     IKDYVTPQQLADQILFMCSPRGKTISGQAISICGDTQMLG"
     gene            complement(2751..4373)
                     /locus_tag="VGA15_13105"
     CDS             complement(2751..4373)
                     /locus_tag="VGA15_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588665.1"
                     /translation="MPSDPAVSIKDLRIALPPGGERAYAVDGVSFDLEPGEIVCLVGE
                     SGSGKSMCAHALMGLLPDSVTTETGEIAFEGNNLLALDADGWRDLRGRRIAMVFQEPM
                     TALNPLMRIGEQMMEMFEAHRLLSPCERRQKALGLAREVGLPDPARIVRAYPHQLSGG
                     QRQRAMIAMALALEPAVLVADEPTTALDVTTQAQILKLIRDLQRSRNMAVMFITHDFG
                     VVADIADRVVVLRYGQIVEQGSAQAVLNRPQHAYTKALLAAVPSMTPPPRTPLIDRNK
                     AVDVIGIDKTYITSGGWFRPDRTVQAASEVSFSILKGETLGLVGESGSGKSSVARLVM
                     RLIEADRGTIRIGEVDLTQLEGKALRDQRRRIQMIFQDPFASLNPRRKVGQIISDGPI
                     AHGTDPKLARQRARDLLGMVGLDAGAMERYPHEFSGGQRQRIGIARALALDPEILVAD
                     EAVSALDVSVQAQVLNLLEDLKTRLGLSMLFITHDLRVAAQICDRIAVMQRGVIVELK
                     ATAALFAAPEHVYTRELLAAVPGQQKPVPLSA"
     gene            complement(4376..>4919)
                     /locus_tag="VGA15_13110"
     CDS             complement(4376..>4919)
                     /locus_tag="VGA15_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588666.1"
                     /translation="AGYFGGRIDDALMRFTEFFQTIPSFALAIVLVAILQPSIYSIVA
                     SIAIVSWPPVARLVRGEVLSLRSREYVQAAIVTGQTSRWIIWHEILPNALSPVIVLAS
                     LMVATAILLESSLSFLGLGDPNLISWGYMVGAGRTVIRQAWWITVFPGLAILISVLGL
                     NLVGEGLNDALNPRLARDGR"
BASE COUNT          914 a         1525 c         1580 g          900 t
ORIGIN      
        1 gcccttctcg aaatgctcga tgatctttgg cgattccgtc gatttgatct cgttgacggt
       61 ctgcgccacg atcttggtgg cggtgtagtc gccccaggcc tggttttccg gcggcttgtt
      121 atatttcttg gtgaaggccg ccacgaaggt cttggagccg ggcgtgtcga tcagatgatg
      181 ccagaccacc ggccaggtgc cgacgaagtt gcccttgccg gcggcccagg caagtgccgt
      241 gtcgaagccg aacccgccga ccgggaaggt caagccgaac tcggcatatt gcttgaggaa
      301 gttggtgatc tggttgccgg cgagattgat cacgaccaga tcgggcttgg cctcgcggat
      361 tttcaacagg taagcggaga aatcggtggc gtcggtcggc actagatcgt cgccggcgaa
      421 attgccgcca ttggcttcca tgaagcgctt tgaaactttt aataggtcat ggccgaacgc
      481 gtaatcggcc gtcagcgaat accacttctt gcccttgacg aggccgtcgc gcaggaacga
      541 gcggccgact gccttgacat acatcgaatt ctgcgactcg acatggaaca tgtagcgctg
      601 gcaatccttg ccgcgcagcg catcggagtt gccgccggtg ttgatgaaca gcgtcttgtt
      661 gcgcgaggcg acctgcgcga tggtcagaca cgaggcggag gaaatctctc cgatgatgca
      721 ggccaccttg tcgcgctcga tcatgcgctc ggccttggtc gaggcggtct gcggattgac
      781 cgagtcttcc ttgagcaact cgaccttgcg gcccatcatg ccgcccgacg cgttgatctc
      841 ttccaccgca aggtcgatcg ccatcaccgc atattcgccg agcggcccga ggaagccggt
      901 gcgcggcgtc aggtgaccga tcctgatgac atcagcggtc tgcgcgcgca aaatcgccgg
      961 cgcggaaatt cccgatgcca atgcaatgcc gccggcggtc ttcaacaggt cgcggcgcgt
     1021 gaattgattg ttgtgaaaca tcagccttgg tctccctgat acggccgcgt ggactgcggc
     1081 tgtctgttct tcggcaggcg gcgtagcccc tggggcgcgc tttgtcttgc cgtgatctgt
     1141 gatcacagtt agagttgcaa aagaccgggc cgttgtcgag tcctctctcg aaattcgcct
     1201 gccatgggag aaacgcgttg aatttgcccg ccgcgctcaa tctggtggag ccgctggacc
     1261 gtcagacgct gggcgagcgc gcctatgcga aactggccga tctcctgatt tccggccggc
     1321 tggcgccggg cgaaaaactg tcgctgcggg cggcggcgga tgtgctcggc gtctccatca
     1381 tgccggtacg cgaggcggtg tcgcggctgg tagccgacaa ggcgctggag gtgacgccga
     1441 accgcgcggt gcgcgtgccc ttgatgtcgg cggcgcaatt tcgcgatctc accaaggtgc
     1501 gcatcgccat cgaaggccat gccgcggcgc aagcagcgct gcatcgggac aacaccgacc
     1561 tcgggttgat cgcgcgcgca gaagaggcga tgaaggcgga aagccaatcc cccgcgcccg
     1621 atctgccgcg cgcggtcgaa ctcaacaaga cctttcattt cgcggtttat gaagcggcgc
     1681 actcgccgat cctggtcgaa atcatccgcg cgctctggct taaagccggg ccggtgatca
     1741 atctcgatct gcgcgccaat cccgaacggc tggcgaaggg cgacgcgatc cggtttcacg
     1801 ccgatgtgcg caaggccatc gccgccggcg ccggcgaagc ggcgcttgag ggaataaaag
     1861 ccgacatcag cagtgccgcg gatttcattt tgtcccgcgg tggtctggcc ggctgacact
     1921 tattgcagag acatctcgga gcgatcatgg atctcgaact caagggctta cgcgtgctgg
     1981 tcaccgccgg tgccaacggc atcgggcttg caattgcgcg tgcttttgcc ggcgaaggcg
     2041 ccagggttca cacctgtgac gtcgatgaaa cggcgttggc cgcactcgcg gcaagcgatc
     2101 ccgcgatcac gcagacatcc tgcgatgttt ccgatcgcgc cgcggtaaag aatctgttcg
     2161 cggagacggt cgcaaaactg ggcggcctcg acgtgctcgt caacaatgcc ggcatcgccg
     2221 gaccgacctc cagggtcgag gagatgaatc cggaagattg ggaccgctgt ctggagattt
     2281 gcctcaccgg ccaattcaac tgcacccggc tcgcggtgcc gctgttgcgc cagagcaaga
     2341 acgcctcaat cgtcaatatc tcgtcggcgg cgggccggct cggctttgcg atgcgtaccc
     2401 cctatgccgc ggcgaaatgg ggcgtgatcg gactgacaaa atcgctgtcg atcgaactcg
     2461 ggccggataa catccgcgtc aatgcgattc tgcccggcct cgtcgccggc gaccgccagc
     2521 gccgtgtgct ggaagccaaa gcgcagcagc gcgggatttc ctacgccgag atggagcgca
     2581 ccgcattttc ctacacctcg atcaaggact atgtgacgcc gcaacaactc gccgatcaga
     2641 tcctgttcat gtgcagcccg cgcggcaaga cgatttcggg tcaggcgatc tcgatctgcg
     2701 gcgacacgca gatgctggga taggcgacgc gcggacacat aatgggatgt tcaagccgac
     2761 aggggaaccg gcttttgctg gccgggcacg gccgcgagaa gttcacgggt gtagacatgc
     2821 tcaggggccg cgaacaatgc cgccgtggct ttaagttcga cgatcacgcc gcgctgcatc
     2881 accgcgatgc ggtcgcagat ttgcgccgcg acacgcagat catgggtgat gaacagcatc
     2941 gacagcccaa gccgggtctt caagtcttcc agcaagttca gcacctgcgc ctgcaccgag
     3001 acatcgagcg cggagacggc ctcatccgcg acaaggattt ccggatcgag cgccagtgcg
     3061 cgcgcgatgc cgatgcgttg ccgttgtccg cctgagaact cgtgggggta gcgttccatc
     3121 gctccggcat cgagaccaac catgcccaga agatcgcggg cgcgctgtcg cgccagtttc
     3181 gggtcggtgc catgcgcgat cggtccgtcg ctgatgattt gtccgacctt gcggcgtgga
     3241 ttgagcgagg caaacggatc ctgaaagatc atctggatac ggcggcgctg gtcgcgcagc
     3301 gccttgccct cgagctgagt cagatcgacc tcgccgatcc ggatggtgcc gcgatcggcc
     3361 tcgatcagcc gcatcaccag acgcgccacc gatgacttgc cggagccgga ctcgccgacc
     3421 aggccgagcg tctcgccttt cagaatcgag aagctgactt cgctggcggc ctgcaccgtg
     3481 cgatccggcc gaaaccagcc gcccgaggtg atataggtct tgtcgatccc gatcacgtcg
     3541 accgccttgt tgcgatcgat caacggcgtt cgcggcggcg gcgtcatcga gggtaccgcg
     3601 gcgagcagcg ccttggtgta ggcgtgctgc gggcggttga gcaccgcctg tgcgctgcct
     3661 tgctcgacga tttgcccgta ccgcagcacg acgacgcgat cggcgatatc ggcgaccacg
     3721 ccgaaatcgt gagtgatgaa catgaccgcc atgttgcggc tgcgttgcag gtcgcggatc
     3781 aattttagaa tttgcgcctg cgtggtgacg tcgagcgcgg tggtcggctc gtccgccacc
     3841 agcaccgcgg gctccagtgc cagggccatc gcgatcatcg cccgctgacg ctggccgccg
     3901 gacagttgat gcggataggc gcgcacgatc ctcgcagggt cgggaagccc gacttcccgt
     3961 gcgaggccca gcgccttttg ccggcgttcg cacggcgaca gcaggcggtg cgcctcgaac
     4021 atttccatca tctgctcgcc aatccgcatc aagggattga gcgccgtcat cggctcctga
     4081 aacaccatgg caatgcggcg tccgcgcaga tcgcgccaac cgtccgcgtc aagcgccagc
     4141 agattgttgc cttcgaacgc gatctcgccg gtttcagtgg tgacactgtc cggcagcagt
     4201 cccatcagcg cgtgcgcgca catcgatttg ccggagccgg attcaccaac caagcagacg
     4261 atttcgccag gctcaagatc gaacgaaacg ccgtcgacgg cgtaggcgcg ctcgccgccg
     4321 gggggcagcg caattctcaa atccttgatg ctgacggcgg gatcggaggg catggtcagc
     4381 ggccatccct tgcaagccgc ggattgaggg cgtcgttgag gccctcgcca accagattta
     4441 atcccagcac ggagatcagg atggccagcc ccgggaacac cgtgatccac catgcctgcc
     4501 ggatcacggt gcggccggcg ccgaccatgt agccccacga aatcagattg ggatcgccga
     4561 ggccgaggaa cgacagcgag gattccagca ggatcgcggt cgcgaccatc agcgacgcca
     4621 gcacgatgac aggcgagagc gcgttgggca ggatttcgtg ccaaatgatc catcggctgg
     4681 tttggccggt gacgatggcg gcctggacat attcgcggct gcgcaatgac agcacctcgc
     4741 cgcgcaccag ccgcgccacc ggcggccagc tgacgatagc gatggaggcg acgatcgaat
     4801 agatcgacgg ctgcaggatc gccaccagca cgatcgcaag tgcgaagctc ggaatggtct
     4861 ggaagaactc ggtgaagcgc atcagcgcgt cgtcgatgcg gccgccgaaa tagccggcg
//
LOCUS       DASWGM010000453         5706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2125645,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000453 DASWGM010000000
VERSION     DASWGM010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125645"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1309
                     /locus_tag="VGA15_13115"
     CDS             278..1309
                     /locus_tag="VGA15_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9588667.1"
                     /translation="MKMSHGRACTRLITTFALLSGISPSHAQKADPAAVAEHVAAATA
                     AAKSDLLGPLGLCKTATPTPGPSFMDNYRAMQKKPPLEPMQVMDELYFLGSRWTTAWA
                     IKTAGGIIIVDAMDNADEAEHYIEGGLRKLGLDPAEIRYVVISHAHGDHHGGAQYLKQ
                     KFNPRLVMSEIDWNALEETQRDPLFGTKRNPLFGEPPKRDMAVADGDTIQLAGTALKF
                     YVIPGHTLGTLATVFTVHDRGEPHRAVAWGGTAYNFGPLPDRLQLYSDTTIKYREILK
                     QENADIFLSNHVSFDDAVAKMAALKDRAPGQPNAFVVGPDAVQRFLTVLGECALATKA
                     SIGEDTPGR"
     gene            complement(1326..2201)
                     /locus_tag="VGA15_13120"
     CDS             complement(1326..2201)
                     /locus_tag="VGA15_13120"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9588668.1"
                     /translation="MNVTQLRISRRSITMAAAFLGMVSLAIGAHAALNMRALDAAKAV
                     EAEQTGALKGASRIALVIGNGNYPDASAPLAQPINDARALTAALRHNGFDVDVVEDAG
                     KDDMNRAIGRLKSKIRHDSVVMLFFGGYGVQVGRESYMIPVDAVIWKEADVRRDGFSI
                     EQVLDVMKEQGARAKLVVIDASRRNPYERRFRKFSHGLAPINAPDNALILTSATPGKV
                     ADDSAGPTSVLVTELLNNLNANLDTKTAAGVETVFNKTRVAISRASEGEQVPSVSSSL
                     SEDVRLEAANANVGG"
     gene            2960..4696
                     /locus_tag="VGA15_13125"
     CDS             2960..4696
                     /locus_tag="VGA15_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588669.1"
                     /translation="MKFRDIAGTAYRSLTARKDGPSLYDVCDPLLLKPSGGDPHLTKF
                     YRTALGNPALRPLLRRAGLPELRDAPRLRSLSQALIRARDDENPDWAAIGAPVASLLD
                     TITMQHPKPKPAPAPARAPDLAEIESVIRTCGTHLLRSFGKNGFIPTYAAFNLMGDPD
                     VGGREVLMALNGLDARSYKNSTLLFNLARIFIARSPARALIRPAWTGIAEPMWQPMQI
                     RHRSAYYDAFFTEALLSFVDTGLASRSETAASRRAIAEMVDFCLTTSREDVRGQDGGT
                     VSVVTALAPLPHPRFSRFFTQIKQDLGFGIYVPDCDTTACALSAATQAGSNDPILAQP
                     LLDFYAGYQVGGSANEPKVTVPLNDNIDYEGGVVTWIDNLAGERPFGNDLDPTLNLDI
                     LEVSFRNLARWKIIETPQRLDTVHRIIGFQKRLVASGAFTNPRSHIYYLPELYCAYFG
                     RCYAAFVALPPAAQKAIDPDGAYEFIRAKVLAYVQTELIAGEMNAFDAALALIALCHL
                     GADAKTFIPALHCIVSSLGEGGRHGPFKAYEWNKMKTPTRILVGGPEATSAFVLMGLA
                     LARRTMMRGAGL"
     gene            4951..5679
                     /locus_tag="VGA15_13130"
     CDS             4951..5679
                     /locus_tag="VGA15_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX9588670.1"
                     /translation="MFMKFLFALLVLLLPALAEAADVSGVPKISEGDQIQIGNSRIRL
                     GGIDAPSVDQLCLNNAGERWTCGVAARDELIKHADNKTWTCHTRQVVDRRGRTVARCE
                     VDGEDIQKWMVRNGWALSYVRVSHDYDDDEKAAREAKAGMWQGAFIAPWDWRARNKKT
                     AILGAAKPVENANTILLASASGSVAPSPDCTIKGNVNRAGECIYHQPTSRWYAKIKMQ
                     ISKGTRWFCSVDEAEAAGCRETKR"
BASE COUNT         1087 a         1854 c         1698 g         1067 t
ORIGIN      
        1 ctcctacttt gatcggcgcc ccctcgaact caaggccggc cggctcgttg ctcgtcttaa
       61 aaagctcggc tttaacgtcc aactccaacc agcagcggag gccgcctgac tcctctatcc
      121 aacagcttct tcctaggagc gaagcgacga agcaatccac tcttcgtttt tccgctcggg
      181 attgcttcgt ggagcctgtc atcgggcgcg cattcgcgcg acccgttggc tcgcaatgac
      241 gatcttaatt ccctttaatt tcccttaggt aaaaccaatg aaaatgtcgc acggtcgtgc
      301 gtgcacgcga ctcatcacca cattcgctct cctgtccggc atctccccaa gccatgccca
      361 aaaagccgac cccgccgccg tcgcggaaca tgtcgccgcc gccaccgcgg ctgcgaaatc
      421 ggatctgctc ggaccgcttg gactgtgcaa aaccgcgacg cctacgccgg gcccgagctt
      481 catggataat tatcgcgcca tgcagaagaa gccgccgcta gaaccgatgc aggtgatgga
      541 cgaattgtat tttctgggtt cgcgatggac cacggcatgg gcgatcaaaa ccgccggcgg
      601 catcatcatc gtcgatgcca tggataatgc cgacgaggcc gagcactata tcgagggcgg
      661 cctgcgcaag ctcgggctcg atcccgccga gatccgctat gtcgtgatca gccatgccca
      721 tggcgatcac catggcggcg cgcaatatct caagcaaaaa ttcaatccgc gcctggtgat
      781 gagcgagatc gactggaacg cgcttgagga aacccagcgc gacccgctgt tcggcaccaa
      841 gcgcaatccc ctgttcggcg agccgccgaa gcgcgatatg gcggttgccg acggcgatac
      901 catccagctc gccggcaccg cgctgaaatt ctatgtcatc cccggccaca cgctcggcac
      961 gctggccacc gtgttcacgg tgcatgaccg tggcgagccg caccgtgccg tggcatgggg
     1021 cggcacggcc tacaatttcg ggccgctgcc cgaccggctg cagctctatt ccgacaccac
     1081 gataaaatac cgcgagatcc tcaaacagga aaacgccgac atcttcctgt ccaaccatgt
     1141 cagcttcgac gacgccgtgg caaaaatggc ggcgctgaaa gatcgcgcgc cgggtcagcc
     1201 caatgcgttc gtggtcgggc cggatgcggt gcagcgcttc ctgaccgttc tcggcgaatg
     1261 cgcgctggcg acaaaagcca gcatcggcga ggacacgccg ggcagatagc ggctggaaac
     1321 gctagctagc cgccgacgtt tgcgttcgcc gcttcaaggc gaacgtcttc cgatagcgac
     1381 gacgacaccg atgggacctg ctcgccctcg gacgcgcgcg aaatcgcaac gcgggtcttg
     1441 ttgaacacgg tttcgacacc cgcggcggtc ttggtgtcaa ggttggcatt gaggttattg
     1501 agcagctcgg tcaccagcac gctggtcggc cctgcggaat cgtcggcgac cttgcccggc
     1561 gtggccgacg tcaggatcag cgcattgtcc ggcgcattga tcggcgccag accatgggag
     1621 aatttgcgga accggcgctc gtacggatta cgccgcgagg cgtcgatgac gacgagcttg
     1681 gcgcgggcgc cctgctcctt catcacatcc aggacctgct caatgctgaa gccgtcgcgg
     1741 cgcacgtcgg cttccttcca gatcaccgcg tcgaccggga tcatgtagct ttcgcggccg
     1801 acctgaacgc cgtagccgcc gaaaaacagc atgaccacgg aatcgtgcct gatcttcgat
     1861 ttcagccggc cgatcgcccg gttcatgtcg tccttgccgg cgtcctccac gacatcgacg
     1921 tcaaagccgt tgtgccgcag cgccgccgtc agcgcccgcg catcgttgat cggttgcgcc
     1981 agcggcgcgc tggcgtcggg atagttgccg ttgccaatca ccagggcgat ccgcgacgcc
     2041 cccttcaggg cgccggtctg ctccgcttcg accgccttgg ccgcgtccag cgcccgcatg
     2101 ttcagcgcag catgggcgcc gatcgccagc gacaccatgc ccaggaaggc ggcggccatg
     2161 gtgatcgagc ggcgggagat gcgaagctgg gttacattca tccggttcat tcctggtcag
     2221 tgtcaaaggc gcgcgcgcca gttcgtcgcg ccaaagccat ctaggtttgt tgggggtgcc
     2281 cgatcctgtg ccgcccaatt gcgctcaatt tgcggcaccg cttattcaat ttgtggcaat
     2341 gcagcaaaca aaccgttaac ccgcaacgtc ataaacggca acgctggacg gcacttttcg
     2401 atcatgttgg gatggccggt tccattcagt tgccgccaca ttttatcctc gttgtgtgat
     2461 ctatgtcaca ttccagttgt gggctttttc acactgcctt tcaaagactt agaggttatc
     2521 aaagaagcat tcggcagccg ccggttgtgg atgggcgggc ttgggggacg tgcacaaggg
     2581 agcgcacgga aagaatctgc aatgaatctt catcacatcg ccaaggcagc ctgccgagcc
     2641 ctgacccttc gcaaggacgg cccgccgctc caagatgtat gccacccctg cagtgccgca
     2701 ccctgttacc ttgactgcgc gacgaaggat atttgcaacg ctggcgcgaa gccctgatct
     2761 ccgggggtcg ccgggttcaa tcagcgtgac gttgaaatta ttcgaccctg tctgtgataa
     2821 aaatgacaga ggtaccctgt tccggttggt atgcgtttca agcacttgca cgccttggaa
     2881 ccggcgggcg gcaagctctt gatacggatt tggccgggta tacgggacgt gcgacaagat
     2941 cgcacaggga agagacacca tgaaatttcg agacatcgcc gggaccgcct accgttcgct
     3001 gacggctcgc aaggacggtc cgtcgctgta cgacgtctgc gatcccctgc tgctcaagcc
     3061 ctcgggcggc gacccgcatt tgaccaagtt ctaccggacc gcgctgggga atccggcgct
     3121 gcggccgctg ctgcgccgtg ccggcctccc cgagctgcgc gacgcgccgc gtctgcggtc
     3181 gctcagccaa gccctgatcc gggcgcgcga cgacgaaaac ccggactggg ccgcgatcgg
     3241 cgcgccggtc gcgtcgctcc tggacacgat caccatgcag caccccaaac caaaacccgc
     3301 gccggcgccg gcgcgcgcgc ccgatctggc cgagatcgaa agcgtcatcc gcacctgcgg
     3361 gacgcatctg ttgcgatcgt ttggcaaaaa cggtttcatt ccgacctatg ctgccttcaa
     3421 cctgatgggc gacccggatg tcggcggccg cgaagtgctg atggcgctga atggtctcga
     3481 tgcgcgcagc tacaagaatt cgacgctgct gttcaatctg gcgcggatct tcattgcgcg
     3541 ctcgcccgcc cgcgccctca tcaggccggc ctggaccggc atcgccgagc cgatgtggca
     3601 gccgatgcag atccgccatc gctccgccta ttacgacgcg tttttcaccg aggcgctcct
     3661 gagctttgtc gacaccgggc tcgcctcccg gagcgagact gcagcgtcgc gccgcgccat
     3721 cgccgagatg gtcgatttct gtctcaccac cagccgggag gatgtgcgcg ggcaagacgg
     3781 cggtaccgtc agcgtcgtca ccgcgctggc gccgctgccg catccgcgct tttcccggtt
     3841 cttcacccag atcaagcagg atctcggttt tgggatctac gttccggatt gcgataccac
     3901 ggcttgcgcc ctctcagcgg cgacgcaggc cggctccaat gatccgatcc tcgcgcagcc
     3961 gctgctcgat ttctatgccg gctaccaggt cggcggcagc gccaacgagc ccaaggtgac
     4021 cgtgccgctc aatgacaaca tcgattatga aggcggcgtc gttacctgga tcgacaacct
     4081 cgctggcgaa cggccgttcg gcaacgatct cgatccgacg ctgaacctcg acatcctcga
     4141 agtcagtttt cgcaatttgg cgcgatggaa aatcatcgag accccgcagc ggctggacac
     4201 cgttcaccgc attatcggct ttcaaaaacg actggtcgcg agcggcgcat tcaccaatcc
     4261 gcgctcgcac atctattacc tgccggagct ttattgcgcc tatttcggcc ggtgctatgc
     4321 cgccttcgtc gcacttcccc cagcggcaca gaaggcgatc gaccccgatg gcgcgtacga
     4381 attcatccgg gccaaggtgc tcgcttacgt gcagaccgaa ctgatcgcgg gcgaaatgaa
     4441 cgcattcgat gcggcgctgg cgctgatcgc gctctgccat ctcggcgcgg acgcaaaaac
     4501 cttcatcccg gccctgcatt gcatcgtcag cagtctcggc gaaggcggcc gccatggacc
     4561 gttcaaggcc tatgaatgga acaagatgaa aaccccgacc cgcatcctgg tcggcggccc
     4621 cgaggccacc tcggcgttcg tgctgatggg tcttgcgctg gcccgccgga cgatgatgcg
     4681 gggcgcgggc ttgtaaggtc tgcgtgcctc caacgttggc tgtcataccc cgcgaaagcg
     4741 ggtatccagt acggcgcagc ttctcgattc gatcgcaagc gctctggaat tactggatca
     4801 cccgctttcg cgggtgacga cggtggtaag cgtagtccat cgcgttaagt tgaaagcgac
     4861 cggccgaccg ggcgctggtg tgccaaacca atccccacca caatccgctg ccatgataat
     4921 atcagcccag attgaatccg accgctttgg atgtttatga aattcctgtt cgcgcttctg
     4981 gtattgctgt tgccggcgct ggccgaggcg gccgacgtca gcggcgttcc gaaaatcagc
     5041 gagggcgatc agatccagat cggcaatagc cgcatccgtc tgggcggaat cgatgcgccc
     5101 tcggtcgacc agctttgcct caacaatgcc ggcgagcgct ggacctgcgg cgtcgccgcg
     5161 cgcgacgagc tgatcaagca cgccgacaac aagacctgga cctgtcacac ccgtcaggtc
     5221 gtcgaccggc gcggccgaac agtggcgcgg tgcgaggtcg acggcgagga catccagaaa
     5281 tggatggtga ggaacggatg ggcgttatct tatgtgcgcg tctcgcatga ctatgatgac
     5341 gacgagaagg ccgcgcgcga ggccaaggcc gggatgtggc agggtgcctt catcgcgccc
     5401 tgggactggc gagcccgcaa caagaagacg gccattctcg gggccgccaa gccggtcgag
     5461 aacgccaaca cgatcctgct ggcgtcggcg tcgggatcgg tcgcgccctc gcccgactgc
     5521 accatcaaag gcaacgtcaa ccgtgcaggc gaatgcatct atcaccaacc caccagccgc
     5581 tggtatgcga agatcaagat gcaaatctca aagggcacgc gctggttctg ctcggtcgac
     5641 gaggccgaag ccgccggctg ccgcgagacc aaacggtaac gtgttttcga gcgaaagcct
     5701 gccgcg
//
LOCUS       DASWGM010000454         8289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2665537,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000454 DASWGM010000000
VERSION     DASWGM010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8289
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2665537"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VGA15_13135"
     CDS             <1..841
                     /locus_tag="VGA15_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX9588671.1"
                     /translation="ASQDSAIAVTALKAGAADYLVKDVQGDFIPLLQVAVNGALRQAR
                     IQKARDDAEAEVHASRDRYAALAAEREVLLREVNHRVGNSLQIIASLLHLQANSSTQE
                     DVKAALTNAMGRVAAVAQVHRRLYTSHDLKSVLLNQYLDALLEDLRRSAEGNKMSRLT
                     LKAEPVEIDPDRAVAIGIIVNELVMNAVKYAYPDGAGPIHVDLHAQGDDLLLSIADDG
                     VGLNVKLDPRSTGMGQRIVSAMAAKLEASVERDPGHAGTRILLRFRRVNTPATKPASV
                     AAS"
     gene            876..1556
                     /gene="aat"
                     /locus_tag="VGA15_13140"
     CDS             876..1556
                     /gene="aat"
                     /locus_tag="VGA15_13140"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX9588672.1"
                     /translation="MTSRDSASSEITPEVLLRAYACGIFPMAESADDPTLFWVEPEMR
                     GVIPLDGLRIPSRLARTVRSDAFTVTVNTAFKAVIAGCAAPQPGRDDTWINKRIRDLY
                     IGLHVLGHGHSVEVWQNDDLVGGLYGVSLGRAFFGESMFHRARDASKVALVHLVARLI
                     AGGFQLLDTQYVTEHLRSLGAIEISRRRYRGLLDKAVAGEADFRKLPVDRPIGGAQAL
                     EIIAGRAS"
     gene            complement(2184..3296)
                     /locus_tag="VGA15_13145"
     CDS             complement(2184..3296)
                     /locus_tag="VGA15_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017996016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9588673.1"
                     /translation="MHDPPRILIVDDNETNRCLLTARLGAEGYETMEAENGERALAVV
                     REVAPDVVLLDVMMPKIDGFEVCRRLKGDTTLGLVPIIMVTARTDSKDVVTGLNAGAD
                     EYLTKPIDHAALVARVRSMLRIKELHDRVEIQAAELASWNRMLEQRVAEQLVQIERVS
                     RLKRFLSPQVAELILSSSTYEALASHRRQVTIVFGDLRGFTAFAEIAEPEEVMAVMRE
                     YHATIGSLIHEFEGTLERFLGDGIMVIFGDPIPCPDPCERAVQMAVAMRGRLSELSSK
                     WRHERHELGFGVGIAYGYATLGAIGFEGRSEYSAIGTVVNLAARLCAEARDGQIFIDS
                     KVRAALDGRVTAKPVGELTLKGLHRPIAAYNVLEMT"
     gene            complement(3326..3691)
                     /locus_tag="VGA15_13150"
     CDS             complement(3326..3691)
                     /locus_tag="VGA15_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9588674.1"
                     /translation="MSKRVLVVEDQEDNRQILRDLLASVGYEMIEAYDGEGALVAART
                     QNPDLILMDIQLPVLDGYEATRRIKAQSDLRHIPIVVVTSYALSGDEEKARAAGCDAY
                     VAKPYSARKLLETINRYLT"
     gene            complement(3688..5286)
                     /locus_tag="VGA15_13155"
     CDS             complement(3688..5286)
                     /locus_tag="VGA15_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9588675.1"
                     /translation="MNLEADSSTAFLAQRPLAAISRASADGALVKDGLVDFRLRPSLR
                     TKLLAAFLVIEVLLVSVGVIGLLSLREADQHANQVVALQHKIEAYRQVQHDTLRQLYG
                     VSTALGFPNETTLAGALRQINQFGYDLDRVSFVAKDEVALLNQLREEYARFIAVVSHV
                     VDLIRNGRSAEAKQSELAELGPLADRLERLTNQLVNRAEADMVAGLDTSRQTYAKSQI
                     LVAAVALASFVLTLVLGHAISRSVIDPVRVIHHGLNRIASGDFTQRIEVPNRDELGEL
                     ATHVNSTCEELQELYQSLEQASRHKSQFLANMSHELRTPLNAILGFSELLLDGIYGDP
                     PEKMRSAVERIQRNGRHLLGLINDVLDLSKIEAGQLRLSLADYSVEELVSGVYTSVES
                     LAAEKSLGLRIAVPPGLPPARGDERRLAQALFNLVGNAIKFTDAGEVRIEVEAKGDSY
                     KFSVQDTGPGIDEADQTKIFQEFQQVDNSITKTKGGAGLGLAIVKRIVEMHGGRIWIE
                     SRLGHGANFSFLVAARLEQQATRT"
     gene            complement(5270..6283)
                     /locus_tag="VGA15_13160"
     CDS             complement(5270..6283)
                     /locus_tag="VGA15_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588676.1"
                     /translation="MHTNRRELLLAGACAASTALFWSAAARLRAEEPNPPPETTTIRL
                     AKNPSICIAPQYVVSDLLNAEGFTNVVYVESDAGVGQSKALANGDLDFTLHFSGPLLL
                     EIDRGLSITLLAGIHVGCFELFAKEGIRSVADLKGRTVGQQGLGSSPHVFLSAMATFV
                     GLNPAKDIEWVTSASVKPIELFAEGKIDAFLGFPPEPQRLHAQNIGHVIVNSAQDRPW
                     SQYFCCMLAGNREFVRKNPIATKRMVRAMLRATDLCVSEPALVAQRMVDRGFTPRHDY
                     AVQTLADVPYNRWRDYDPEDTIRFYALRLREAGMLKSGPAKIVADGADWRFLKEVRRE
                     LGG"
     gene            complement(6539..7489)
                     /locus_tag="VGA15_13165"
     CDS             complement(6539..7489)
                     /locus_tag="VGA15_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEX9588677.1"
                     /translation="MFRTIALTSIAAFVAATTISLAPPAHAQIGNIFSEPAPRPPGNI
                     PRGGNQPQPDDEEEVPELPQGRLLPTPNRPPPGQGIPAPGNIQSQPLAPPPGTTVIPQ
                     NAPPGIAVAPPQPGQGVAVAPPGAAPLPGQKLPKGVPQSPATLQPGDEVVSEPPAQKI
                     VNKKASFSGLDKITGRIINFDEDIGETVQFGALRVKTDACYTRPATEAANTDAFVEVD
                     EITLQGEVKRIFSGWMYAASPGLHGVEHPIYDIWLTDCKAPDTTVVSAQPDAPRPAPP
                     PAQKRAPPRQAQRSPPQFQQPPQDLPPPQRQPGLFPGFRQ"
     gene            7723..>8289
                     /locus_tag="VGA15_13170"
     CDS             7723..>8289
                     /locus_tag="VGA15_13170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9588678.1"
                     /translation="MPDRIRLDGKVAVVTGAAGVIGTATMRLLAERGARIVAVDRKAA
                     DLQRAVKDLPAAAQALAVPADVTSEDEVADYVRAAVDQFGTIDVFYNNAGIEGDVAPI
                     TKYSLEMFRKVIDVNVVGVFLGLKHVLPVMLKQDRGSIINTASIAGLIGSPHIAVYSA
                     SKHAVIGLTKSAAWECTATGVRVNCVCPG"
BASE COUNT         1616 a         2575 c         2494 g         1604 t
ORIGIN      
        1 ggcctcgcag gactcggcga tcgccgtcac cgcactgaag gcgggcgccg ccgactatct
       61 ggtcaaggac gtccagggcg atttcatccc cctgctccag gtcgccgtca acggcgcgct
      121 gcggcaggcg cggatccaga aagcccgcga cgacgccgag gccgaggtcc acgcctcgcg
      181 cgaccgctat gccgcgctcg ccgccgaacg cgaggtgctg ctgcgcgagg tcaaccaccg
      241 ggtcggcaac agcctgcaga tcatcgcctc gctattacac ctgcaggcca attcgagcac
      301 ccaggaagac gtcaaggcgg cgctgaccaa tgcgatgggg cgggtcgccg cggtggcgca
      361 ggttcaccgc cggctttaca cctcgcacga cctcaagagc gtgctcttga accagtatct
      421 cgacgcgctg ctcgaggatc tcaggagatc cgccgagggc aacaagatgt cgcggctgac
      481 cttgaaggcc gagccggtgg agatcgatcc ggaccgtgcg gtggcgatcg gcatcatcgt
      541 caatgagctc gtgatgaacg ccgtgaaata cgcctacccc gacggcgccg ggcctattca
      601 tgtcgacctc catgcgcagg gcgacgatct cctgctgtcg attgctgacg acggcgtcgg
      661 cctcaacgtc aagcttgacc cgcgttccac cgggatggga cagcgcatcg tctccgccat
      721 ggccgccaag ctcgaggcca gcgtcgaacg cgaccccggc catgccggca cccggatcct
      781 gctgcggttt cgccgcgtca atacgccggc aaccaagccc gcgagcgtgg cagcgagcta
      841 gccgccgccg ccctccccct gctatcctgt cgcgcatgac atcgcgcgac tcggcctcct
      901 ccgaaattac gcccgaagtg ctgctgcgcg cctatgcctg cggcatcttc ccgatggccg
      961 aaagcgccga cgatccgacc ctgttctggg tcgaaccgga aatgcgcggc gtgattccgc
     1021 tcgacggcct tcgcatcccc tcgcgtctgg cgcgcaccgt gcgctccgac gccttcaccg
     1081 tcaccgtcaa tacggcgttc aaagcggtga tcgcgggctg cgcagcacct cagccgggcc
     1141 gcgacgacac ctggatcaac aagcgcatcc gcgatctcta catcgggctg cacgtactcg
     1201 gacatggcca cagcgtcgag gtctggcaga atgacgacct tgtcggcgga ttgtacgggg
     1261 tcagcctcgg ccgcgccttc ttcggcgaga gcatgtttca tcgcgcccgc gacgcctcga
     1321 aggtggcgct ggtgcatcta gtcgcgcggc tgatcgccgg cggcttccag ttgctcgaca
     1381 cgcaatatgt gaccgagcat ttgcgcagcc ttggcgccat cgagatctcg cgccggcgct
     1441 accgcgggct gctcgacaag gcggtcgcgg gcgaagcgga tttccggaaa ttgccggtcg
     1501 atcggccaat cggcggcgcg caggcgctgg aaatcatcgc gggacgcgcc agttgacacc
     1561 gcgcaacgga aacccttgac ctcaggccgc cttgctctcg atacgtccca ggtgtgaggg
     1621 tggtttggcc agcagcccgg gaagcagcgc gtctgccgca agagcgcccg agatcagcgc
     1681 ggcttccacg ccgggtccgt acgtcgaact cccggccaca accatgccgg ggatcggact
     1741 cttgaccccc tctcagccga tccttcaggc ggatgagatc atgatccgat tcaaaccaat
     1801 cggacctagg gcgtgagctc ataaattcag agacgttggc gaacgtccgc tttggagcgc
     1861 attccggact caagtcggac atcgcgcaag gtccgaaaag tgtgccacag cagattagcc
     1921 gatctaattc gaatacctca tccgcgcgag ctattgagct tggtggaact tcaacaccta
     1981 cgggcttggc cgctttcaaa ttgatcgcga gctcgaatct ggttggttgc acgacaacag
     2041 acaggtctgg aggcagctcg acagaaagca ttcgatctgc atctttccct ctttgccgat
     2101 atgcgtcgat aagaatggga aaacgcatcg gagaaggcaa cgggcgggtt gcaacgatgc
     2161 aaacttggtc gtcgacatct ccctcaagtc atctcaagca cgttgtacgc cgctattgga
     2221 cgatgcaaac ctttgagcgt cagttcacct acgggctttg ccgtcaccct tccgtcgagg
     2281 gccgcgcgca ctttgctatc gatgaaaatc tggccgtcgc gcgcctccgc gcaaagacgg
     2341 gccgcgagat tgaccacggt cccgatcgcg ctgtactcag agcggccctc gaaaccaata
     2401 gcgccgagcg tcgcatatcc atatgcgatg ccgactccaa aaccgagctc gtgcctttcg
     2461 tgccgccact tgctggataa ttcggacagg cggccccgca tcgcgaccgc catctgaaca
     2521 gctctctcgc acggatcggg acaaggaatc ggatcgccga aaatcaccat gattccgtcg
     2581 cccaggaatc tctccaacgt gccttcaaat tcatggataa gagagcctat ggttgcatga
     2641 tactcccgca tcacggccat cacctcctcg ggctcggcga tctccgcaaa ggcggtaaat
     2701 ccccgcagat caccgaagac tatggtcacc tgcctgcggt ggctcgccaa ggcctcataa
     2761 gtcgaggaag aaaggatcag ttccgctacc tgtggtgaaa gaaaacgctt taagcggcta
     2821 acccgttcaa tttgcacaag ctgctcggca acgcgttgct caagcattct attccacgac
     2881 gcgagttcgg ccgcctggat ttccactcga tcatgcaatt ccttgattcg gagcatcgag
     2941 cgcacgcgcg caacgagtgc tgcatggtcg atgggtttgg tcagatattc gtcggcgcct
     3001 gcgttgagtc ccgtgacgac atcttttgag tcagttcgcg ccgtaaccat aatgattgga
     3061 acaagtccaa gcgtggtgtc gcccttcagt cgccggcaga cctcaaagcc atcgattttc
     3121 ggcatcatga cgtcaaggag gacaacgtcc ggcgcaacct cacgcacgac ggcgagcgcc
     3181 ctctcgccgt tttcagcctc catcgtctca taaccctcgg cgccgaggcg cgcggtaaga
     3241 aggcagcgat tcgtttcgtt atcatctaca atcaaaattc ggggtgggtc atgcatgtct
     3301 gctccacgtc ggcacgcgcc ggaacctatg tcaggtatcg attgatcgtc tcaaggagtt
     3361 ttcgtgcgct gtaaggcttc gcgacatagg catcgcagcc tgccgcgcgc gccttttctt
     3421 catcgccgct aagcgcgtag gaggtgacga cgacgattgg aatatgacgg agatctgatt
     3481 gtgctttgat acgccgtgtc gcctcgtatc catcaagaac aggcagctgg atatccatca
     3541 gaataagatc tggattttgg gttcgcgccg cgaccagtgc tccttcgccg tcgtacgcct
     3601 cgatcatttc gtaaccgacg cttgcaagca gatcgcgcag aatctgccga ttgtcctcct
     3661 gatcttcgac gactagcacc cgcttgctca tgtccgcgtc gcctgttgct caagccgggc
     3721 tgccaccaaa aaagagaagt ttgcgccatg accgaggcgg gattctatcc agattcgtcc
     3781 accatgcatc tccacaatgc gctttacgat cgcgaggccc aatcccgcgc ctcccttcgt
     3841 cttggtgatc gaattatcca cctgctggaa ttcttggaaa atttttgttt gatcggcttc
     3901 gtcgatgccc ggtcccgtgt cttggacgga aaatttgtag gaatccccct tcgcctccac
     3961 ttcgatccga acctctcccg catcggtgaa tttgatcgca ttaccaacaa gattgaacag
     4021 ggcctgagcg agcctgcgtt cgtctcctcg cgccggcggc aagccagggg gaacagcaat
     4081 tctcaaaccc aaactctttt cggcggcgag cgattcaacg gaagtataaa cgccgctaac
     4141 caattcctct acggaatagt ccgccaaaga aagtcgaagc tgtccggctt caatcttgga
     4201 gagatcgagg acatcgttga tgagcccgag cagatgtctg ccgttccgct ggatgcgttc
     4261 gacggctgat cgcatttttt ccggaggatc gccgtagatg ccgtccagca ggagctccga
     4321 aaagccgagt atcgcgttca gtggcgtccg caattcgtga ctcatgtttg cgaggaactg
     4381 cgacttgtga cggctggcct gctcaaggct ttggtagagc tcctgtagct cctcgcaggt
     4441 cgaattgacg tgcgtcgcaa gctccccaag ctcgtcccga ttgggaacct cgatgcgctg
     4501 ggtgaagtcg ccagacgcta ttcgattcag accgtggtgg ataacgcgga ctgggtcgat
     4561 aacagatcgg gagatcgcat gtcccaagac cagcgtaagc acgaaacttg cgagcgcgac
     4621 ggcggcgaca agtatctgag atttggcata ggtttgccgg ctcgtatcta ggccggcaac
     4681 catgtccgct tcagcccggt tgaccaattg attggtcaat cgttcgagcc tgtccgcgag
     4741 gggcccgagt tccgcgagtt cgctctgttt agcctcagcc gatcgcccgt tgcggatgag
     4801 atcgacgacg tgcgaaacta ccgcaatgaa gcgcgcatat tcttcgcgca gctggttgag
     4861 cagcgcaacc tcgtcttttg cgacgaacga gacgcggtca aggtcgtagc caaactggtt
     4921 gatctgccgc agcgcacccg ccaatgtcgt ttcgttcgga aacccgagag ctgtggatac
     4981 gccatagagc tgtcgaagcg tatcgtgctg cacctgtcgg tatgcctcga tcttgtgctg
     5041 cagggcgact acttggttag cgtgctggtc ggcttctcgc agggaaagga gcccgattac
     5101 cccaaccgag accaagagca cctcgatgac gaggaaggcc gctagcaatt tggtgcgcag
     5161 cgaggggcgt aatctaaagt cgacgagacc gtccttcacc aacgcgccgt ctgcacttgc
     5221 tcgcgatatt gcagcgagcg ggcgctgagc cagaaaggcg gtagatgaat cagcctccaa
     5281 gttcacgtcg aacctctttg aggaaccgcc agtcggctcc atccgcaacg attttcgcag
     5341 ggcctgattt cagcatgccc gcttcgcgca gacgaagcgc gtaaaaacgg attgtgtctt
     5401 cgggatcgta gtcacgccaa cggttgtagg gcacatctgc gagagtctga accgcatagt
     5461 cgtggcgggg cgtgaacccc ctatctacca tacgctgcgc aacaagggcc ggctcgctca
     5521 cgcacaaatc cgtcgcccgc agcatggcac gaaccatacg tttggtggcg atgggattct
     5581 ttcggacgaa ttcgcgattc cccgccaaca tgcagcaaaa atactgcgac catgggcgat
     5641 cctgcgcgct gttgacgatg acatgaccga tgttctgtgc atgcaaacgc tgtggttctg
     5701 gaggaaatcc gaggaaggcg tcgatcttgc cctcggcaaa caactcgatc ggcttgaccg
     5761 acgcgctcgt cacccactcg atatccttgg cggggttaag tccaacaaac gtcgccatcg
     5821 cgctgaggaa aacgtgcgga ctggacccaa gcccctgctg gccgaccgtt cgacccttca
     5881 gatcggcgac gctgcgtatg ccctccttgg cgaatagctc gaagcagccg acatgaatcc
     5941 cggcgagaag agtaatgctc aagccccgat cgatctcaag aagaagcgga ccggagaaat
     6001 gcaaagtgaa gtcgagatcg ccgttcgcga gtgctttcga ttgtcccacg ccagcgtcag
     6061 attcaacgta aacgacgttt gtgaacccct cggcattcag caggtcgctc accacgtact
     6121 gcggagcgat gcagatgctc gggttcttcg caaggcggat tgtggtggtt tctggcggcg
     6181 gattgggctc ttcagccctg agccgagccg cagcgctcca aaatagggcg gtggaagcgg
     6241 cgcaggcccc agccaacagc aattcgcggc ggttcgtatg catcgggacc tcctcgaaat
     6301 cacctgagat gaacggtggc acttccgggg gccgaacgtc aggccgacgt atgttgcgtg
     6361 gacctcaagg cccgctggca acgaagatac cacggctgcg gcatgtcgga tatgtccgtg
     6421 tgcctgacgc gttcgcggta acctgatatc cttgagcgcc gctggtattt tctaagcgac
     6481 gccgggccgt cttacctcgt ccagcttgcg gggcgaggtt tagaaagcga agctcgcttt
     6541 actgcctgaa ccccgggaac agacccggct gtcgctgcgg cgggggcaga tcctgcggcg
     6601 gttgctggaa ttgcggcggc gaacgctgcg cctgcctcgg cggcgcgcgc ttctgagcgg
     6661 gcggcggcgc cggccttggc gcatccggct gcgcgctcac gaccgtggtg tcaggggctt
     6721 tgcagtcggt cagccagatg tcgtagatcg ggtgctcgac accatgcagg ccggggctcg
     6781 ccgcatacat ccagcccgaa aaaatccgct tcacctcgcc ctgcagcgtg atctcgtcga
     6841 cctcgacaaa ggcgtcggta ttggcggcct cggttgccgg acgcgtgtag caggcgtcgg
     6901 tcttgacgcg cagcgcgccg aactggacgg tctcgccgat atcctcatcg aagttgatga
     6961 tgcgcccggt gatcttgtcg agcccggaaa agctcgcctt cttgttgacg atcttctgcg
     7021 ccggcggctc ggaaaccact tcgtcgccgg gctgcaatgt ggccggcgat tgcggcacgc
     7081 ccttgggcag cttctgcccc ggcagcggag cggcgcccgg cggcgccacg gcgacgccct
     7141 gtccgggctg cggcggcgcg actgcgatgc ccggtggcgc attctgcgga attacggtcg
     7201 tgcccggcgg cggcgccagc ggctgcgact ggatgttgcc cggcgcgggt atgccctgcc
     7261 ccggcggcgg acgattgggt gtcggcagca ggcgaccctg cggcagttcc ggcacttcct
     7321 cttcgtcgtc gggttgcggc tgattgccgc cgcggggaat attgccgggc ggacgcggcg
     7381 ccggctcgga aaatatattg ccgatctgcg cgtgcgccgg cggcgcaagc gagatcgtgg
     7441 tggcggcaac aaaggccgca atactggtca gggcaatggt tcgaaacatc tcgcggcttt
     7501 taagggcgaa tcgggctcgc gacattctat agacttatcc gccgatcgca ggggccccgg
     7561 ttaacacgcc gaatacggcg gggaaagggc ggtttaccgc cggttccggc cactggccag
     7621 cccccgccac agatgaaata gtcgccccag gaacgttcgc gcgccctgcg ccggaccgcc
     7681 agaaatccgg aaacaaccgg aacagcagag gaaacgtaac ccatgcccga ccgaattcgt
     7741 ctggacggca aggtcgccgt cgtcactggg gccgccggcg tcatcggcac cgccacgatg
     7801 cggctgttgg ccgagcgcgg cgcgcgcatt gtcgcggtcg accgcaaggc ggccgatctg
     7861 caaagagcgg tgaaggatct gccggccgca gcgcaggcgc tcgccgtgcc tgctgacgtc
     7921 accagcgagg acgaggtcgc cgactatgtc cgcgccgccg tcgaccagtt cggcaccatc
     7981 gacgtgttct acaacaacgc cggcatcgag ggtgacgtcg cgccgatcac gaaatattcg
     8041 ctggagatgt ttcgcaaggt gatcgacgtc aatgtggtcg gtgttttctt aggcctgaaa
     8101 catgtgctgc cggtgatgct gaagcaggac aggggcagca tcatcaatac cgcatcgatt
     8161 gccggcttga tcggatcgcc gcacatcgcg gtctacagcg ccagcaagca tgcggtgatc
     8221 gggctcacca agagtgcggc gtgggaatgc accgcgaccg gggtgcgcgt gaattgcgtc
     8281 tgccccggc
//
LOCUS       DASWGM010000455         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2406779,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000455 DASWGM010000000
VERSION     DASWGM010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2406779"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VGA15_13175"
     CDS             complement(<1..401)
                     /locus_tag="VGA15_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9588679.1"
                     /translation="MRHSLGRSIRAGGLLLAALLLTTAAAAQTGGNEGLSPTLANIKN
                     THVVRLGYRESSPPFSFLDQANRPIGYSLELCEAIVDEIGVEVDDPNLRIEYVKVTSD
                     DRIPAVVQNKIDLECGSTTANAERGKQVAFSP"
     gene            complement(414..1709)
                     /locus_tag="VGA15_13180"
     CDS             complement(414..1709)
                     /locus_tag="VGA15_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX9588680.1"
                     /translation="MSNRFTQYILIAMALGIVMGTLVFNYLPDIRADIAADVNLIAML
                     FLRLIKMIIAPLVFATLVGGIAHMGGGARLGRIFAKTMGWFVSASFVSLLLGLVMVNL
                     LQPGANFPGTLPDKAQSTGLPVSAFSIEKFLTHLIPTSIADAMAQNEILQIVVFAVFF
                     SVALGAMPERSKQILSVIDDLGHIMLKVTAYVMLFAPIAVWAAIMATVSKNGLAVLWK
                     LVVFMGGFYLSLMILWGILVIVGFIVIGPRYSHLLRLIREPLMIAFSTASSEAAYPKT
                     LEGLNRFGASSRISSFVLPLGYSFNLDGTMMYCTFASIFIAQTYHIEMSLGTQFAMLA
                     TLMITSKGVAGVPRASLVVIASTLGQFGIPEAGLLMIMGIDTFLDMGRSATNVIGNSL
                     ATAVVAKWEGELAPEHALGSDDVVPPDMIPGEIPAMAGH"
     gene            1922..>2875
                     /locus_tag="VGA15_13185"
     CDS             1922..>2875
                     /locus_tag="VGA15_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX9588681.1"
                     /translation="MTEGVNRQVLLVEKPTGKLAPEHFKMSEGAIPEPKDGEALLRVR
                     YISLDAANRAWMHGATYRAALEANTVMAGGGIAEVVSSKAPGLAPGDIVFGDTGWQDY
                     AAVPAKHLTKMPKVEPMTHLLSVYGIAGLTAYFGLLDVGKPKAGETVVVSAAAGSVGS
                     IVGQIAKIKGCRVVGIAGGNDKCSWLTSELGFDAAVDYKDGATFKALKAAAPKGIDVY
                     FDNVGGDILEACLAQMNNRGRIACCGAISQYDGAPSAHGPRGVPGLIVVKRLIMQGFI
                     VMDFMDQRDEALTDLQSWVASGKLKVQEDVINGLENTPKALI"
BASE COUNT          593 a          876 c          900 g          506 t
ORIGIN      
        1 ggcgagaacg ccacctgctt gccgcgctcc gcattggccg tggtcgatcc gcattcgaga
       61 tcgatcttgt tctgcaccac cgccggaatg cggtcatccg aggtcacctt gacgtattcg
      121 attctgagat tgggatcgtc gacctcgaca ccgatctcat cgacgatggc ttcgcagagt
      181 tcgaggctgt agccgatcgg ccggttggcc tgatcgagga acgagaacgg cggcgaactc
      241 tcgcgataac ccagacgcac gacatgcgtg ttcttgatat tcgcaagcgt cgggctcagc
      301 ccttcattgc cgccggtctg ggcggcggcg gccgttgtca gcagcaacgc cgccaacaac
      361 aggccgccgg cccggatcga tctccccaga ctatggcgca tgcatcgctc ccatcaatgg
      421 ccggccattg cggggatctc gccggggatc atatccggcg gcaccacgtc gtcggagcca
      481 agcgcgtgct cgggcgcaag ttcgccctcc cacttcgcca ccacagcggt tgcgagcgag
      541 ttgccgatca cattggtggc gctgcgtccc atgtcgagga acgtatcgat gcccatgatc
      601 atcaacagtc ccgcctcggg aatgccgaac tggcccaatg tcgaggcgat caccaccagc
      661 gaagcgcggg gaacgccggc gacgcccttc gaggtgatca tcaaggtcgc cagcatggca
      721 aactgggtgc cgagcgacat ttcgatgtgg taggtctgcg cgatgaagat actggcaaag
      781 gtgcagtaca tcatggtgcc gtcgagattg aaggaatagc cgagcggcag cacgaagctc
      841 gagatccgcg acgaggcgcc gaagcggttg aggccctcca gcgtcttggg ataggcggcc
      901 tccgaactcg ccgtcgagaa cgcgatcatc aggggctcac ggatcagccg caacagatgg
      961 ctgtatctgg gcccgatgac gatgaagccg acgatgacga ggatgcccca caggatcatc
     1021 agcgacagat agaagccgcc catgaacacg accagcttcc acaacacggc gaggccgttc
     1081 ttcgacaccg tcgccatgat cgccgcccac accgcgattg gcgcgaacag catgacatag
     1141 gccgtgacct tcagcatgat gtggccgaga tcgtcgatca ccgagagtat ctgcttggag
     1201 cgttccggca tcgcgccgag cgcgaccgag aagaacaccg cgaacaccac gatctgcagg
     1261 atttcgttct gcgccatcgc atccgcgatc gaggtcggaa tcagatgggt caggaatttc
     1321 tcgatcgaga acgccgacac cggcaggccg gtcgattgcg ccttgtccgg cagggtgccc
     1381 gggaaattcg cgcccggctg cagcagattg accatcacca ggccgagcag cagcgacaca
     1441 aaggacgcgc tgacgaacca gcccatggtc ttggcaaaga tccgcccgag cctggcgccg
     1501 ccgcccatat gggcgatgcc gccgaccagg gtcgcgaaca ccagcggcgc gatgatcatc
     1561 ttgatcaggc gcagaaacag catcgcgatc aggtttacgt ctgctgcgat atccgcccgg
     1621 atatcgggca gatagttgaa gaccagcgtc cccatcacga tgccaagcgc catcgcgatc
     1681 agaatatatt gcgtaaacct gttcgacatt ttttaccccc gtaggttccg ttgcttgcga
     1741 gtttggcaga ttccggcagc gacgcaacac gcttggaagt accgggcagt ttgccacaat
     1801 gttcccgttg tggaatggac gcgcgccgac tagcctcggg cgcagcgcac aatctgtgca
     1861 gttgagcgtt ttctgcgcgg ccagttgcat caagaaccgc caaatgaggg agggaaatgc
     1921 catgactgag ggtgtcaatc gtcaggtctt gctggttgaa aagccgaccg ggaaacttgc
     1981 accggagcat ttcaaaatgt ccgagggcgc catccccgaa ccgaaagacg gcgaggcgtt
     2041 gctgcgggtg cgctatattt cactggatgc cgccaaccgg gcctggatgc atggcgccac
     2101 ctatcgcgcg gcgctcgagg ccaatacggt gatggccggc ggcggcatcg ccgaagtggt
     2161 atcctcgaaa gcgccggggc ttgcgccggg cgacatcgtg ttcggcgaca ccggctggca
     2221 ggattacgct gccgtgccgg caaagcacct gaccaagatg ccaaaggtcg agccgatgac
     2281 gcatctattg agcgtgtacg gcatcgcggg gctgaccgcc tatttcggac tgctggacgt
     2341 cggcaagccg aaggccggcg agaccgtggt ggtgtccgcc gcggccggtt ctgtcggctc
     2401 gatcgtcggc caaattgcaa aaatcaaggg atgtcgcgtg gtcggcatcg ccggcggcaa
     2461 cgacaagtgc agctggctga catcagaact cggcttcgat gccgcggtcg attacaagga
     2521 cggcgccacg ttcaaggctc tgaaggccgc cgcgccaaaa ggcatcgacg tctatttcga
     2581 caatgtcggc ggcgacattc tcgaagcctg cctggcgcag atgaacaacc gtggccgcat
     2641 cgcctgctgc ggcgcgatct cgcaatatga cggcgcgccg tcggcgcacg ggccgcgcgg
     2701 cgtgcccggc ctgatcgtgg tgaagcgcct cattatgcag ggctttatcg tgatggattt
     2761 catggaccag cgcgacgagg cgttgaccga cctgcagtcc tgggtcgcct ccggcaaact
     2821 gaaagttcag gaggacgtca tcaacggcct tgagaatacg ccgaaggcgc tgatc
//
LOCUS       DASWGM010000456         8993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1000981,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000456 DASWGM010000000
VERSION     DASWGM010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000981"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..81)
                     /locus_tag="VGA15_13190"
     CDS             complement(<1..81)
                     /locus_tag="VGA15_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9588682.1"
                     /translation="MTELLMVRRETHIAAPPATVFAFLTDP"
     gene            complement(164..394)
                     /locus_tag="VGA15_13195"
     CDS             complement(164..394)
                     /locus_tag="VGA15_13195"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX9588683.1"
                     /translation="MTDHKTAIREQWLAVRLELLEAEKEFTRRRDELTRRRMAMPWER
                     VEKSYQFELSHQQWLKANVHFLAREKRAAGLG"
     gene            complement(423..545)
                     /locus_tag="VGA15_13200"
     CDS             complement(423..545)
                     /locus_tag="VGA15_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588684.1"
                     /translation="MEFMHHCSTKWAVFLLSLKSLLETGKGAPAPNEIKLDVWK"
     gene            complement(867..2081)
                     /locus_tag="VGA15_13205"
     CDS             complement(867..2081)
                     /locus_tag="VGA15_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9588685.1"
                     /translation="MNDSRYRWVIVAAGGLLGCVAIGSMFSLPVLLQPIVRNTGWSVA
                     GVSSAMTVGFIALAGASMVWGTLSDRYGPLPVVLTGATVLAASLALASRATSLIEFQL
                     VFGLLVGGATAAIFAPMMACVTGWFDTHRSLAVSLVSAGMGMAPMTMSPLVAWLVTIH
                     DWRTTLQIVAVIVAAIMIPVALLVRRPPALVGDNAAASAGGGPQSEMSLGQAVRSPQF
                     IILLLTNFFCCATHSGPIIHTVSYAVSCGIPLIAAVSIYSVEGLAGLFGRVAFGLLGD
                     RFGAKRVLVTGLLVQAFGALGYVFVRELAAFYAVAALFGFIYAGVMPLYAVLARENFP
                     LKMMGTVIGGTAMAGSLGMATGPIAGGLIYDTFASYAWLYIGAWGMGIGAFLMALTFR
                     PFPKSQPAPVPA"
     gene            complement(2203..2625)
                     /locus_tag="VGA15_13210"
     CDS             complement(2203..2625)
                     /locus_tag="VGA15_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9588686.1"
                     /translation="MSETYTGGCACGAIRYEISGEPMVMNDCQCRDCQRKSGTGHGSY
                     LTFPRRESVKLSGEAKHWDIVGDSGNVKTRGFCPSCGSPVYLTFAAMPDLFTVHAASL
                     DDPSRYKPQMVTYGVRGYAWDRLDPALPKFDKMPPNAS"
     gene            complement(2722..3207)
                     /locus_tag="VGA15_13215"
     CDS             complement(2722..3207)
                     /locus_tag="VGA15_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9588687.1"
                     /translation="MNYAALKSDMHEIVVDEVLPHAPETIWKALTTGELIGRWLMMTP
                     TGFEAVKGKRFTFQTTPGGAWDGVIHCQVLEATPNERLVYAWKSGHEGNVGYGSPLDT
                     VVTFILSRVENGTRLRLVHSGFVLPRNDGAFKKMGEGWKKCVPSIGAIAGEQVSSRKL
                     H"
     gene            complement(3204..3533)
                     /locus_tag="VGA15_13220"
     CDS             complement(3204..3533)
                     /locus_tag="VGA15_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9588688.1"
                     /translation="MIEAAAINAVMRTLADPTRRAVFERIVGSDEITVVELTRGSGVT
                     QGAISQHLKSLKQAGLVAERPEGRNVYYRAEPEGLAPLVDWMSHYGVFWRDRFANLKT
                     LLKEIDP"
     gene            complement(3698..4777)
                     /locus_tag="VGA15_13225"
     CDS             complement(3698..4777)
                     /locus_tag="VGA15_13225"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9588689.1"
                     /translation="MKLPITLAWLLDEASTATGADHFLAALGAKLIADDLPLAGGALT
                     LAAPHPMISRRTWLWRAETGVVIEALGFGALGSAGPGQSDVGRDWLTRLGGAVVQDVA
                     GPASDGPVLGWSILRPLTEQESALLHQAARFAAAPLAVLATRSTLAALLEAYLGRRSA
                     AQVLAGRLRRQTGETIRAVLLFGDLRDFTALSEAMAPEAVVAALDAWFDRIAGAVHAF
                     GGEVLKFIGDGVLAIFPIGERSPSAACEAALSAVGAARAGMTHLDQARERQGLPPLRF
                     GAALHLGEMLWGNIGTADRLDFTAIGPAVNLVSRLEGLCRPLGRSVLISGAVAAETNT
                     RLIPLGEHDLRGIATPCAVFTLPED"
     gene            4997..5446
                     /locus_tag="VGA15_13230"
     CDS             4997..5446
                     /locus_tag="VGA15_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003564639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9588690.1"
                     /translation="MIEPLVVRREVQIAAPPATVFAFLTDPEKILRWMGTEATLEPHP
                     GGLYLVNVTGKDVARGKFTEVIPVHRLAYSFGWDNRKEVPPGSSLIEIDLIDHEAGGT
                     LLRMTHSGLPDAAACANHEQGWIHYLGRLAEAAAGGDPGPDPNRKIA"
     gene            5601..5990
                     /locus_tag="VGA15_13235"
     CDS             5601..5990
                     /locus_tag="VGA15_13235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX9588691.1"
                     /translation="MLTNWISPDALKSLNDGFVVAIMLEAKEGQADALAEILRVLTPP
                     TMAEPGVKLFLPYRSPTNPLKFFIFELYVNEAAWGAHEATDHFKTAVAQLLPTVTRRE
                     RVPFIPFLADAIPAAKLHGSEPKSLLR"
     gene            complement(5979..6662)
                     /locus_tag="VGA15_13240"
     CDS             complement(5979..6662)
                     /locus_tag="VGA15_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007793043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9588692.1"
                     /translation="MKDGPDISITAALIGDPARANMVMALMSGHSLTMGELAAEAGVT
                     LSTASVHLAKLESSRIVVSRKQGRARHFRIADPDVAHCVEALVTVAARAGHLRTRPGP
                     REEAMRHARSCYDHLAGQIAVHLFERWVAAGVLRWRGEEVCLTGKGRQFLIDRGIEID
                     ALERKKRPLCRTCVDWSERKHHLGGAIGAAVLARALAKSWAVRGPRSRAVTFSVLGEK
                     KFIAWYSSQ"
     gene            complement(6715..7770)
                     /locus_tag="VGA15_13245"
     CDS             complement(6715..7770)
                     /locus_tag="VGA15_13245"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9588693.1"
                     /translation="MRRHAVILAAALAWLVSIESAEARLDILVDKATQRMLVIQNGYM
                     RYIWPVSTGRDETATPNGVYTPQRLERNWFSNAYYNSPMPYSIFFHNGYAIHGSYAIN
                     QLGGPASHGCVRLHPHHAALLFDLVQQEGPDKTTIEVSDEARPNEPSLPGREIAAERG
                     LDPPALYPPAPRIVRYRETGEMPELPPEQRGNRPIAARPNEPSLPGREIAAERGVYPP
                     ALYPPVPRIVRYREPGEMPELPPAQRGNRPLAARNALPPRPPAGPPPSTVGRRMVTDV
                     RPPANNVPRAPCGAGEASDESSDCRPKPKVAEASPPEPAQRAPSVYSFKLLPASCWSG
                     GASRWKWWASSQGAPCK"
BASE COUNT         1707 a         2890 c         2747 g         1649 t
ORIGIN      
        1 aggatcggtc aggaaggcga agacggtggc gggcggggct gcgatatggg tctcacgccg
       61 gaccatgagc agttcagtca tcggagtctc cttgattgag gtcgggatag aagtgcccgg
      121 cggtgcgcga cgttccggta ggcgacggga cgatgcggat gcgtcatccg agcccggctg
      181 cccgcttctc cctggccaaa aaatgcacgt tcgccttgag ccattgctga tgcgacagct
      241 caaactggta ggacttttcc acccgctccc agggcatcgc cattcgtcgg cgcgtcagct
      301 catcacgtcg gcgcgtgaat tccttctctg cctccagcag ctccagccgg accgcaagcc
      361 actgttcacg tattgcggtc ttgtggtcgg tcatggtttc tctcctctgg tcggtgcggc
      421 ttttacttcc aaacgtcgag cttgatctcg tttggcgcgg gggcgccttt cccggtttcg
      481 agcagcgact tcaggctcag cagaaagacc gcccacttgg tgctgcaatg gtgcatgaac
      541 tccaccggct ccttccagcc ttggtgcttg aaaagaagcc ttcctgtttg agatcaaaac
      601 ctactgttgt gccgatccat tctgcgggtc cgtcgaccac ttgccacagc acgcgcttgt
      661 tgggttgaag ctcaagaacc ttcatgtcga taccatcggc cccgaagcgg aactgaataa
      721 ctccgccgac tttgcatcat ctggggatga cgatttgatt ccgactttgt gtaaaatacc
      781 gaccctatgc ggttgttcct gctccagtgt ccatgtgcag ctagaacgat tgaggcaccg
      841 aaaaggagtc gaaccccgtc gccgcttcaa gccggcactg gtgctggctg ggatttgggg
      901 aagggcctga aggtcaatgc catcaggaac gcgccaattc ccatgcccca ggcgccgata
      961 tagagccacg cgtagctggc aaaagtgtcg tagatcaagc cgccggcgat tggccccgtc
     1021 gccatgccga ggctgcccgc catggccgtt ccgccgatga cggtgcccat catcttgagt
     1081 gggaaatttt cgcgcgccag cacggcatag agcggcatga cgccggcata gatgaagccg
     1141 aacagcgcgg cgaccgcata gaacgcggca agctcgcgta cgaagacata gcccagtgcg
     1201 ccaaatgcct gaacgagcaa tcccgtgacg agaacgcgct tggcgccgaa ccggtcgccg
     1261 agcaggccga acgcgacgcg accgaagagg cctgcgagcc cttccacgct gtagatcgaa
     1321 acagcggcaa tcaagggtat cccgcagctc acggcgtagc tcacggtatg gatgatcggg
     1381 cccgaatgcg tggcgcagca gaagaaattc gtcagcagca ggatgatgaa ttgcggcgat
     1441 cgcaccgcct gccccaatga catctcggat tgcggtccgc cgccggcgga agcagcggca
     1501 ttgtcgccca caagcgccgg cgggcggcgc accagcaacg cgacgggaat catgatggcg
     1561 gcgacgatga cggcaacgat ctggagcgtg gttctccagt cgtggatcgt gacgagccag
     1621 gcgaccagcg gcgacatggt catgggcgcc atccccattc ccgccgagac cagcgagacc
     1681 gccaggctgc gatgagtatc gaaccagccg gtgacgcaag ccatcatcgg cgcaaagatg
     1741 gcggcggtcg cgccgccgac cagaagcccg aagacaagct gaaactcgat gagcgatgtc
     1801 gcgcggctgg ccagcgcgag gcttgccgcc agcacggttg cgccggtcag cactaccgga
     1861 agcggcccat accggtcgga caaggtgccc cacaccatgc tggcccccgc caaggcaatg
     1921 aaaccgaccg tcatggcact ggacacgccg gcgaccgacc agccggtatt ccgtacgatc
     1981 ggctgaagaa gcacgggcag cgagaacatg gagccgatcg cgacgcaacc gagtaagcca
     2041 ccggcagcga caatcaccca gcgatagcgt gaatcgttca ttctgaatct ccgttccaac
     2101 cgtctccggg ttcaagacga atccgccggc gccgcagacg acagcgcgga tgcgcgcatc
     2161 gcgtcacatc tttgtgagcg aaagccgcgc atgtcgcgtc ggtcaggaag cgtttggcgg
     2221 catcttgtcg aatttcggca gggccggatc gaggcggtcc caggcgtaac cgcgcacgcc
     2281 gtacgtcacc atttgcggct tgtatcggct gggatcgtcg aggctcgcgg cgtgcacggt
     2341 gaacaggtca ggcatggccg caaacgtcag atataccggt gatccgcagc tggggcagaa
     2401 gccgcgcgtc ttcacattgc cgctatcgcc aacgatgtcc caatgcttcg cttcgccact
     2461 gagtttcacg ctctccctgc gcgggaaggt cagataggat ccgtgcccgg tgccgctctt
     2521 ccgctgacag tcgcgacact ggcagtcgtt catcaccatc ggttcgccag agatttcata
     2581 acggatcgcg ccgcaggcgc atccgccggt ataggtctcg ctcatgtctt ctctcctaca
     2641 tctgttctac tccgtgcctg ggacgatcca ggctcggaaa agggtcagcc gctcgaactc
     2701 gctgtcacgg cctgccgcgc gttagtgcag ctttctcgaa gagacctgtt ccccagcaat
     2761 ggcgccgata cttgggacac acttcttcca accttcgccc atcttcttga aagcaccatc
     2821 gttcctcggg agcacgaagc cggagtggac aaggcgaagg cgcgttccgt tttcaaccct
     2881 ggaaaggata aacgtaacga cagtgtccag cggtgagccg tacccgacgt ttccctcgtg
     2941 cccgcttttc caggcgtaga cgagccgttc gttcggcgtc gcttccaaca cctggcagtg
     3001 aatgacaccg tcccacgcgc cgcccggcgt cgtctggaac gtaaagcgct tgcccttcac
     3061 ggcctcgaat cctgtcggcg tcatcatcag ccagcgcccg atcagctcac cggttgtcag
     3121 agctttccag atcgtctccg gcgcatgtgg caggacctca tcaaccacga tttcgtgcat
     3181 gtcagacttc aacgcggcgt agttcatgga tcgatctcct tcaggagggt tttgaggttg
     3241 gcgaagcggt cgcgccagaa cacgccgtaa tggctcatcc aatcgacgag cggcgcgagg
     3301 ccctcaggct ccgcgcgata atagacatta cggccctcgg ggcgctcggc gaccaggccg
     3361 gcctgcttca atgacttgag gtgctgggag atggcgccct gcgtcacgcc gctgccccgc
     3421 gtcagctcga ccacggtaat ctcgtcggac ccgacgatcc gctcgaacac ggcgcgccgc
     3481 gtgggatcgg caagcgtgcg catgacagcg ttgatggcgg cggcttcaat catgccatta
     3541 cattagtaca cactaatcaa ttagtcaatc ctaatttgtc gcgacttcac agtgccttga
     3601 tcgctgatct ctgcaacact ccatatccat cggcggtcgc gtggctcggc gcgggagatc
     3661 gggtcactcg ccggtacgat ggccgaccaa gcagatgtca atcctccggc agtgtgaaga
     3721 ccgcacaagg cgtggcgatg ccgcgcaggt cgtgctcacc cagcgggatc aacctcgtgt
     3781 tcgtctccgc tgcgaccgcg cccgagatca gcacgctgcg gccgagcggc cggcacaacc
     3841 cttcgagccg gctgaccaga ttgacggcgg ggccgatcgc ggtgaaatcc agccggtcgg
     3901 cggtgccgat gttgccccac agcatctcgc cgagatgcag tgctgcgccg aagcgcagag
     3961 gcggcaggcc ttgccgttcg cgtgcctggt cgagatgggt catgccggcc cgggccgcgc
     4021 cgacggcgct gagcgcggct tcgcaggcgg cggaaggcga ccgttcgccg atgggaaaaa
     4081 tcgcaagcac cccgtcgccg atgaatttca gcacctcgcc gccgaaggcg tgaacggcgc
     4141 cggcgatgcg gtcgaaccag gcgtcgagcg cggcgaccac cgcctccggc gccatcgcct
     4201 cggacagagc cgtgaaatcg cgcagatcgc caaagagcaa gaccgcgcgg atggtctcgc
     4261 ctgtctgtcg ccgcagccgc cccgccagta cctgcgccgc gctgcgtcgg ccgagataag
     4321 cctcgagcag tgccgcgagg gtcgaccgcg tggccaacac ggcgagcggc gccgcggcga
     4381 aacgtgccgc ctggtgcagc agcgcggatt cctgctcggt gagcggccgc aagatcgacc
     4441 agccaagcac cgggccgtct gacgcaggac cggcgacatc ctgcaccacc gcgccgccaa
     4501 gtcgcgttag ccaatcgcgg ccgacatcgc tctgcccggg cccggccgag cccaaagccc
     4561 cgaagcccag cgcttcgatc accacaccag tctccgcccg ccacagccag gtgcggcggg
     4621 agatcatcgg atgtggcgcc gccagcgtca gggctccgcc ggcgagcggc aaatcatcgg
     4681 cgatcagctt ggcgccgagc gcggccagga aatggtcagc gccggtcgcg gtgctggcct
     4741 cgtcgagcag ccacgcaagg gtgatgggca acttcatgcg gggacaatgc ggccgcggcg
     4801 gagacctgtc accatagcat agttacccgg gtaggctcgt ggctaagaat atgcttttat
     4861 caagaacgga gccttgctcc gcgctatcgc cggtccgacc cgtcgccgca tcctggcgcg
     4921 gaagcgtgct cgctcgtttc agtcgcgcgc tcggagaccg agcatcgcac cacgctattg
     4981 tcgaaggagg cttccgatga ttgaaccgct cgtggtccgg cgcgaggtcc aaatagcagc
     5041 gccgccagcg accgtcttcg ctttcctcac cgatccggaa aagatcctgc ggtggatggg
     5101 cacggaggcg acgctggaac cgcatcccgg cgggctttac ctcgtcaacg tcaccggcaa
     5161 ggacgtcgcc cgcggcaagt ttaccgaggt cattccggtc caccgcctcg cctacagctt
     5221 cgggtgggac aaccgcaaag aggtgccgcc cgggtcgagc ctgatcgaga ttgacctgat
     5281 cgatcatgag gcgggtggaa cgctgctgcg catgacccat agcggcctgc ccgacgctgc
     5341 ggcctgcgcc aaccacgaac agggctggat ccattacctc ggaaggctcg ccgaagccgc
     5401 cgccggcggc gatccgggtc ccgaccccaa ccgcaagata gcctgacgca ttttgcgcgc
     5461 gttcacaaac ttgttttcga cgtcatgcat gcggtcaatc gcgtccccaa tactttgacc
     5521 gtcatctaac catcctgtgc tgctgccgtg ctagccatgc cggcacccca agccatcgaa
     5581 cccaggccat ggaggcacag atgctcacca attggatctc gcccgacgcg ctgaaatccc
     5641 tgaacgacgg cttcgtcgtt gcgatcatgc ttgaagccaa agaggggcag gcggacgcgc
     5701 ttgccgagat tctccgggtc ctgacgccac cgacgatggc ggagcccggc gtcaagcttt
     5761 tccttcccta ccgctccccg accaacccgc taaagttctt tatcttcgag ctctacgtca
     5821 acgaggccgc ctggggcgcg catgaggcga ccgaccattt caagaccgcg gtcgcgcagc
     5881 ttctgccaac ggtcacgcgg cgcgagcgcg tgcccttcat tccgtttctc gccgacgcaa
     5941 tcccggcggc gaagctgcat ggctcggaac cgaaaagcct attgcgatga ataccacgcg
     6001 atgaatttct tctcgccgag cacgctgaag gtgacggcgc gtgagcgcgg cccgcgaacc
     6061 gcccagctct ttgccagcgc ccgcgccaga accgccgctc cgatggcgcc gccgagatga
     6121 tgctttcgct cgctccaatc gacgcaggtg cggcagagcg gacgtttctt gcgctcaagt
     6181 gcgtcgatct cgatgccgcg gtcgatgagg aattgtcgcc ccttccccgt gaggcagacc
     6241 tcctcgccgc gccaccgcag cacacccgcc gcgacccagc gctcgaacag atgcacggcg
     6301 atttgcccgg cgagatggtc gtagcaggat cgggcatgac gcatcgcctc ttccctgggg
     6361 cccggcctcg tccgcagatg cccggcccgc gccgccactg tcaccagcgc ctcgacgcaa
     6421 tgcgcgacgt cggggtcggc gatccgaaaa tggcgggcgc ggccttgttt ccggctgacg
     6481 acgatgcgtg aggattcgag cttggcgaga tgcacgctcg ccgtcgacag cgtcaccccg
     6541 gcctcggcgg ccagttctcc catggtcagc gaatggcccg acatcagggc catgaccata
     6601 ttggcgcgcg ccggatcgcc gatcagggct gccgtgatcg aaatgtcggg gccgtctttc
     6661 atggtttgac gatgatcaaa ccatgctatc ccgccaaggt acaggcgcga gaaattactt
     6721 gcatggagct ccctggctcg atgcccacca cttccatcgc gacgcgccgc cggaccaaca
     6781 ggaggcaggc aggagcttga agctgtaaac cgatggcgct cgttgcgccg gttctggcgg
     6841 cgatgcttcg gcaaccttcg gtttgggacg acagtcggaa gattcgtccg aggcctcccc
     6901 tgctccgcat ggtgcgcgcg gcacgttgtt ggcgggcggc ctgacgtcgg tcaccatacg
     6961 acggccgacc gtcgacggcg gcgggccagc cggcggcctc ggcggcaagg cattcctcgc
     7021 cgcaagtggc cgattgccgc gctgtgccgg gggtagctca ggcatctcac cgggttcccg
     7081 ataacgcacg atcctcggca cgggcgggta cagcgcgggc gggtacacgc ctcgttccgc
     7141 cgcgatctca cgccccggca atgacggctc gttagggcgt gcggcaatcg gccgattgcc
     7201 gcgctgttcc gggggtagct caggcatctc accggtttcc cgataacgta cgatcctcgg
     7261 cgcgggcggg tacagcgcgg gcgggtccaa gcctcgttcc gccgcgatct cacgccccgg
     7321 caatgacggc tcgttagggc gtgcttcgtc cgacacttcg atggtcgtct tgtccggacc
     7381 ttcctgttgc accaggtcga acagcagtgc ggcatgatgg ggatgcagcc gtacgcagcc
     7441 atgcgatgct ggtcccccca gttgattgat cgcgtagctg ccgtggatcg cgtagccatt
     7501 gtggaagaag attgaatatg gcatcggcga gttgtaatag gcgttggaga accagttgcg
     7561 ctccaatcgc tgcggagtgt agacgccgtt tggggttgcc gtctcatcgc ggccggtcga
     7621 gacgggccag atatagcgca tgtaaccgtt ctggatgacc agcatgcgct gcgtcgcctt
     7681 atcgacaaga atgtcgagcc ttgcctcggc gctctcgatg ctgactagcc atgccagtgc
     7741 cgcggcgagg atgacggcat ggcgacgcat gacgaactcc accggttttt gctgcgtcga
     7801 tagagcaaat gaatccgggt gcttttttgg ctaaaatggg accaggaatc tgggcgcagg
     7861 ctggtctgct cgcggaaaga ccgcaaactg cttgtacgcc cggcagttag cgacaggcgc
     7921 caatttattt accatattcc gcaacgctca tcgcgcggct catgctgggc attggtggtc
     7981 ggcattccat cgtttcccgc gcgggattcg attatttgcc tatttactga gcacaattgc
     8041 gtaatagttg cggcgattcg gccgcagcaa cggccctatg atgtggcatc gacccgccgc
     8101 ctgcccaaac agtgtcgaaa taacggcatt ggggcctgcg ccacgcaatg cgcggtccat
     8161 cactgcgcag tgaacgcgaa gggcgtgcct gccaagccac aatatccctt gcgcggctct
     8221 gctgggggcg atcccttgct cggctctgat gggggcggat ctttggtctc ccatcgccac
     8281 atcatgcaac ccgacccctg gcatcggaag aaatttcccc ctcatctcat gatgcagccg
     8341 cgggaaaaat attcgataca gcatgtttct aaagcgggca gagcggcccg gattggagcg
     8401 attatacgca aatggcctgg tttcccagcg aagccggatc atggggcacc acttcttcat
     8461 gactgcgtct tgttcggtca agagcatgcg acttctccat cgacggcgta cgtcgacgcg
     8521 agtgccggct ccgccgccac aaccagcaaa cgctctcgga ggcgtttttc acccatgaga
     8581 tttgtcacat ccgataaatc cccaccgaaa ccagtgatcc gcggaacgaa agttcgaccg
     8641 gccttgaacg ttccaactaa cgccctcaaa aatgccagaa tcagacgtcc ttttagcggc
     8701 aacgccacaa tgatttgcgg cggacgtcgg ggcagaaagc agcgttcata gtccactcta
     8761 gcgccacaat ctaaagcgca cgcgcattgc gagtgaggaa gcatgcttgt caggccacgt
     8821 atctcagacg tggcccttga gcttgcgaca aaatctcgca cgttgccgga tgcgatcaat
     8881 caagcaaggt tttttgcgtt tcgcagcgca aggagatatg cgcagtgaac tttgggtttg
     8941 tgctctcgct cgtactggtg atccttgcgg tcgttggcgt gttcatcgag ata
//
LOCUS       DASWGM010000457         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_51907,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000457 DASWGM010000000
VERSION     DASWGM010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51907"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VGA15_13250"
     CDS             complement(<1..1271)
                     /locus_tag="VGA15_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX9588694.1"
                     /translation="MAGFVAGLDRGQATLLPECLEDWVDENNSVRAVDVFVEALDLRD
                     IGFEGVDAAGIGRPAYHPSPMLKLYIYGYLNRVQSSRRLEREAGRNLEVMWLTGRLAP
                     DHKTIADFRKDNGPAIRKVCARFVELCRKMGLLAKASVAIDGSKFKAVNSRDNNFTQG
                     KIQRRQKQIEESVARYMSQLDTADRQTAAGEDPSETVLLIKTRLKEKLAKLEEEVKRL
                     AAIEKALLASPDKQISLTDPDCRSMATSGRGSGMVAYNVQSAVDTTNHLIVAHEVTNV
                     GTDRSQLATMAQAAKAALRSDNLDVVADRGYFKGEEILACEQAGVAVTLPKPQTSGAK
                     SAGRFGKPDFVYLAKDDVYRCPAGEKLAHHFTADEDGQKMRIYLTKACRTCPLKDQCT
                     TANERRIKRWEHEHVIEAAQTRLDQNPQAMRV"
     gene            1477..2331
                     /locus_tag="VGA15_13255"
     CDS             1477..2331
                     /locus_tag="VGA15_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEX9588695.1"
                     /translation="MRWCVSVGAVLVAGAIVGGDVAGIAAPSPKSRFAETKHNVPAVN
                     VELVIAVDVSYSMDMDELAIQREGYAQAIVSKDFLQALRAGPGSKVAVTYFEWSMSGD
                     EKIIIPWRVIDGPESADAVAAEIMKTPVRRGSSTSISGAINFALQLFEENPYRGLRRV
                     IDISGDGPNNDGAPVTSARDAALEKGIIINGLPIMVKEPSYSTIDIENLDLYYEDCVI
                     GGPGAFIMTIKDREKFQEAIRTKLVLEVAGLTPEHGIVPAAEKEPRVPCLIGEKKWQE
                     RRGGRTNL"
BASE COUNT          457 a          724 c          700 g          510 t
ORIGIN      
        1 acacgcatgg cttgcgggtt ctgatcgagc cgcgtctgcg cggcctcgat gacgtgttcg
       61 tgttcccagc gtttgatgcg gcgctcgttg gccgttgtgc actgatcctt gagcgggcat
      121 gtgcggcacg ccttcgtcaa ataaatccgc attttctggc catcttcatc agcggtgaag
      181 tggtgcgcca gcttctcgcc ggccgggcag cgatagacat cgtccttggc caaataaaca
      241 aaatcaggtt tcccaaagcg gcctgccgac ttggcgcccg aggtttgcgg cttgggcagc
      301 gtgaccgcga cgccggcctg ttcgcacgcc aggatttcct cacctttaaa gtagccccga
      361 tccgcaacga cgtccaagtt atcgctgcgc agcgcggctt tcgccgcctg cgccatcgtc
      421 gccagttgcg atctatcggt gccgacgttg gtgacctcgt gcgcgacaat cagatggttc
      481 gtggtgtcga ccgcactttg cacattgtag gcgaccatgc ctgaaccgcg cccactcgtt
      541 gccatcgagc ggcaatcggg atcggtcagc gaaatctgct tgtcgggcga ggcgagcaat
      601 gccttttcaa tcgcggccag ccgtttcact tcttcttcga gcttcgccag cttttcctta
      661 agccgcgtct tgatcagcag caccgtttcc gacggatctt ctcctgcggc agtctggcgg
      721 tcggcggtgt cgagttggct catgtagcgc gccacactct cttcgatctg cttctggcga
      781 cgctggatct tgccctgcgt gaagttgttg tcgcgactgt tcacggcctt gaacttgctg
      841 ccgtcgatgg caacgctggc cttcgccagc aggcccatct tgcggcacaa ctcgacaaat
      901 cgcgcgcaaa ccttcctgat cgcgggacca ttgtccttgc ggaagtcggc gatggttttg
      961 tgatccggcg cgagccgtcc ggtcagccac atcacctcaa gattgcggcc ggcctcgcgc
     1021 tccagccgcc ggctcgattg gacccggttg agatagccgt aaatgtatag cttgagcatc
     1081 ggcgaaggat ggtacgccgg ccggccgatc cctgccgcat cgaccccttc aaagcctata
     1141 tcgcgcagat ccagtgcctc aacaaacaca tcgaccgcgc gaacagaatt gttttcgtcg
     1201 acccaatcct caaggcattc tggcagcaac gtcgcctgcc cgcgatccag ccccgccacg
     1261 aagcccgcca tcaaatcccc cgccgattca gtaagggaat catagcatgg caggagtttt
     1321 cacacaggct ggacccgaag cggacatttc cgggcttcta ctcattgtcc cagatcacgc
     1381 ctcgattgct gcgaacgtga acgacaattg cgtgtttccg cttttcgcgg cgcgttttgg
     1441 atgtcaccat cctcgtgaaa ggacgggagg gccgccatgc gctggtgtgt ctcggtcggg
     1501 gcggtgcttg ttgcgggcgc gatagtcgga ggtgacgttg ctggcattgc cgcgccgagc
     1561 ccgaaatctc gattcgccga aaccaagcac aatgtaccag ccgtaaacgt cgagctcgtt
     1621 atcgccgtcg acgtctccta ttcgatggat atggacgaac ttgccattca gcgcgagggc
     1681 tatgcgcagg ccatcgtctc gaaagacttc cttcaggcgc tgagggccgg tccgggcagc
     1741 aaggtggccg tgacttattt cgagtggtcg atgtcgggcg acgagaaaat catcattccc
     1801 tggcgagtga tcgacggccc ggaatcggcg gatgccgtgg ccgccgagat catgaaaaca
     1861 ccggttcgtc gaggttcgag tacctcgatt tccggtgcga ttaatttcgc actgcagctg
     1921 ttcgaagaga atccctatcg cgggttgcgg cgcgtcatcg atatttctgg cgatgggccg
     1981 aacaacgacg gagcccccgt aaccagcgcg cgcgacgctg ccctggagaa gggcatcatc
     2041 atcaacggcc tgccgatcat ggtgaaggag ccatcctatt cgacgatcga tatcgagaat
     2101 ctagatctct attacgagga ctgcgtgatc ggagggccgg gcgccttcat catgacgatc
     2161 aaagatcgcg aaaaattcca ggaagcgatc cggaccaagc ttgttctcga agttgcgggc
     2221 ctcacgccgg agcacgggat cgtcccagcc gccgagaaag agccccgggt gccttgtctg
     2281 atcggcgaaa aaaaatggca ggagcgcagg ggcggacgaa caaatttgta gacagcacta
     2341 tcatgatcgc agccagcgcc cacgcgccgg cattggtggc gctaacgttc g
//
LOCUS       DASWGM010000458         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2407020,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000458 DASWGM010000000
VERSION     DASWGM010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2407020"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1222
                     /locus_tag="VGA15_13260"
     CDS             146..1222
                     /locus_tag="VGA15_13260"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588696.1"
                     /translation="MKRRAFISLLGGASVWPLAARAQQSSTPIIGLLNGRSIDDAAYL
                     LVAFRQGLREAGYVEGQNVTIEYRWANGDYDRMPAMAVDLVRRQVAVIAAIGDPSPFV
                     AKGASTTIPIVFLSGSDPVRRGMVASLNRPSGNMTGVSVVAGLLVAKRIQLLREMAPT
                     AAVFGLLVNPNNPNAETETKDSNEAAQTLGLQLRILNASNDREFDAVFATLVQLRPSA
                     LIVGGDAFFNSRSEHLVALAARHAVPTIYNFREFVTAGGLMSYGTNLADGWRLAGVYV
                     GKILKGAKPADLPVENAVKVELAINKKKPRRSASPSRCRCLDAPTRSSNRPRWDNSHC
                     CTCSGLKVALRDHQLGRWNGRLLG"
     gene            1428..>2617
                     /gene="phnA"
                     /locus_tag="VGA15_13265"
     CDS             1428..>2617
                     /gene="phnA"
                     /locus_tag="VGA15_13265"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HEX9588697.1"
                     /translation="MTERNIGEIRVNGRSYRKPTAPTVVVCYDGCDPRYISHGVLAGL
                     LPNISRIMSEGFFAIADAAMPTFTNPNNMSIVTGAPPKVHGISGNYYLDKATGREVMI
                     TDASLVSSGTVLAALADAGVRVAAITAKDKLRKMLGSGLRGICFSSEHAREATLEENG
                     IDEVERLVGRPTPDMYSPDLSLFVLDAGIRLLETHRADVLYLSLSDMVQHSAGPGEEL
                     ADSFNREVDRRVGRMLELGALVAIVADHGMTDKCTPDGQPQVVYLEKALNDQFGPGSV
                     RVICPITDPFVKHHGALGSFVRVYATGSVPAQTLMDAAARIPGVALVLDGESAAARYE
                     MPQEREGDFIAVADTYTAIGSRPDEHDLAGLTGHRLRSHGGLSEQPVPFMLSCPLNET
                     YRAIA"
BASE COUNT          519 a          786 c          810 g          502 t
ORIGIN      
        1 gttaatcgga caaagtcacc gcgtgctcga atcgacgaga ttgaccccac tcggacatga
       61 gtggctgctt ttcgctgcgg tgcacgccct gaatcgctcg aagacctgct ataccagtcc
      121 gtgatcccaa gcttggagga agcgggtgaa acgacgcgcc ttcatctcgt tgctcggcgg
      181 cgcctccgtg tggccgctcg cggcgcgcgc gcagcaatcg tcgacgccga ttatcgggtt
      241 actcaacggc agatcgattg acgatgccgc atatctcctg gtggcgttcc gccaaggtct
      301 cagggaagct ggctatgtcg aaggacagaa tgtgacgatt gaatatcgct gggcgaatgg
      361 tgactatgat cgaatgcccg cgatggcggt cgatttggtt cgtcgtcagg ttgcagtaat
      421 cgcggcaatc ggcgatccgt cgccgttcgt ggcgaagggc gcgagcacga cgattccaat
      481 cgtcttccta tctggcagtg atccagtcag gcgtggcatg gtcgcgagcc tcaaccgccc
      541 cagtggtaac atgacgggcg tgagcgtggt cgcgggattg cttgtggcca agcgtataca
      601 gttgctacgc gagatggcgc cgacagctgc tgtattcggc ctgctggtca acccgaacaa
      661 ccccaatgct gagaccgaaa cgaaagactc gaacgaggct gcacagacgc tcgggctaca
      721 actgcgtatc ctgaatgcca gtaacgatcg agagtttgac gcagtattcg ccacgcttgt
      781 ccaattgcga cccagcgcgc taatcgtcgg cggcgacgcg ttcttcaata gccgcagcga
      841 acacctcgtc gccctggcgg cacgccatgc ggtgcccaca atctataatt tccgcgagtt
      901 cgtcactgcc ggcggcctga tgagttatgg aaccaatctt gctgacggct ggcgtctggc
      961 cggtgtctac gtgggcaaaa ttctaaaggg cgccaagcca gccgacctgc cggtcgaaaa
     1021 cgcggtgaaa gtcgaactgg ctatcaacaa gaagaagcca agacgctcgg cctcaccgtc
     1081 ccgctgccgc tgcttggacg cgccgacgag gtcatcgaat agaccccgtt gggataacag
     1141 ccattgctgc acatgctcag gtctcaaagt ggcactaaga gaccatcaac tgggccgctg
     1201 gaatggccgc ttactggggt aaagcagccg tgccgctggg cagccagata gtctcaagat
     1261 gaccccaagc tgatctcgga ggcgcaggcc catccgctgg ctgcaaaatg tctaacatac
     1321 ctgctgactg atccctgccg gttcgataac ctgctgtggg ctcctgcggc tggtctgcta
     1381 caataggcga ccctcatcat tcggcagaaa agggaggatc ggccgtcatg actgagcgga
     1441 acattggcga aattagagtc aacgggcgga gctaccgcaa acccacggcc ccgaccgttg
     1501 tcgtctgcta tgacggctgc gatccgcgtt acatttcgca cggcgttttg gccggattgt
     1561 tgcccaacat ttccaggatc atgtcggaag gcttcttcgc catcgccgac gccgcgatgc
     1621 cgacattcac gaatccgaac aatatgtcga ttgtcaccgg ggcaccgccg aaggtgcatg
     1681 gcatttccgg caattactat ctcgacaagg caaccggccg ggaggtgatg atcaccgacg
     1741 cctcgctcgt ctcctctggc acggttctgg cggcgcttgc cgatgctggc gtgcgggtgg
     1801 ccgcgatcac agccaaggac aagctccgca agatgctcgg cagcggcctg agggggattt
     1861 gcttctcctc cgagcacgcg cgcgaggcga cgctcgaaga gaacgggatc gacgaggtag
     1921 agcggctggt ggggcgtccg acgccggaca tgtacagccc cgatctctca ctgttcgtgc
     1981 tggatgcggg aatcaggctg ctggaaacgc accgcgccga tgtcctgtat ctgtcgctgt
     2041 cggacatggt ccagcacagc gccggacccg gcgaggaact ggcggattca ttcaatcgcg
     2101 aagtcgacag gcgcgtaggg cggatgcttg agctcggcgc cttggtcgcc atcgtggcgg
     2161 atcacgggat gaccgacaaa tgcactcccg acggccagcc gcaggtggtt tatctggaaa
     2221 aggcgctaaa cgatcagttc ggtccgggct cggtgcgcgt gatctgcccg atcacggatc
     2281 ccttcgtgaa gcatcacggc gccctcgggt ccttcgtgcg cgtctatgca acgggttccg
     2341 tgccggcgca gacgctgatg gacgcagcag cgcgcattcc cggcgtggcg ctggtcctcg
     2401 atggagaaag cgccgccgcg cgctatgaaa tgcctcagga gagggaaggc gatttcatcg
     2461 cggtggccga cacatacacc gcgatcggct cgcggccgga cgagcacgac ctcgccgggc
     2521 tcaccggcca cagactgcgc tcgcatggcg gcctgtcgga gcagcctgtt cctttcatgc
     2581 tgtcgtgtcc cctgaacgag acctatcgcg caatcgc
//
LOCUS       DASWGM010000459         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2268148,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000459 DASWGM010000000
VERSION     DASWGM010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2268148"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGA15_13270"
     CDS             complement(<1..602)
                     /locus_tag="VGA15_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX9588698.1"
                     /translation="MLLAIFADIHANRQAFSACLDFARARGAERMICLGDYVGYGADP
                     EWTIETVMGLVDDGAMAVRGNHDNAVGATAESMNAEAQAAIEWTRGRLSAPQRRFLAE
                     LPFELQEEDRLYVHSEASNPSKWHYVRSASDAARSMVATSAHITFCGHIHQPALYSMS
                     SAAKMTSFVPISGVPVQLLGGRRWLAVLGSVGQPRDRDPAA"
     gene            complement(792..1388)
                     /locus_tag="VGA15_13275"
     CDS             complement(792..1388)
                     /locus_tag="VGA15_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9588699.1"
                     /translation="MNENVVLTPERILEVTEDVLRRFGLAKATVVDVARALDVSHGSV
                     YRHFPSKASLREAVAKRWLDRVNAPLQKIAEGSGPAPARLERWLRTMIEIKHKKVCDD
                     PEMFRTYLTLAQEACKVVKAHKDGLIDQLAHILSDGVKQGVFQVADVKASARAVFEAT
                     SRFHHPAHAEEWSDPQLAARIDAVLALLLRGLEAPRKR"
     gene            complement(1385..1708)
                     /locus_tag="VGA15_13280"
     CDS             complement(1385..1708)
                     /locus_tag="VGA15_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588700.1"
                     /translation="MNVEILYLTAQLNRWLNLQVAHHLEAQAARLKAAKGPVLTTSPK
                     TLGKLGADGPGGCVGLDCRGMPDCGESVEEAEGIDAIPDAAEGTRFPAARMAICDGEK
                     PTVSA"
     gene            1956..>2408
                     /locus_tag="VGA15_13285"
     CDS             1956..>2408
                     /locus_tag="VGA15_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEX9588701.1"
                     /translation="MISRYTRPEMAAIWEAQTRFKIWFEIEAHAADALAELGVIPKEA
                     ARAIWAKARDAIFDVARIDEIERETKHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLD
                     TCLNVQLTRAADLLIADVDKVLVALKKRAFEHKMTPTIGRSHGIHAEPV"
BASE COUNT          452 a          775 c          733 g          448 t
ORIGIN      
        1 gctgccggat cgcgatcgcg gggttggccc accgagccga gaacggccag ccagcgcctt
       61 cccccgagca attgcacggg aacgcccgat atcgggacga agctcgtcat cttcgcggct
      121 gaagacatcg aatagagcgc cggctggtga atatggccgc agaatgtaat gtgagctgac
      181 gtcgccacca tgctgcgcgc ggcatccgat gcactgcgca cgtaatgcca tttggaggga
      241 ttgctggctt cggaatgaac gtagaggcga tcctcctcct gcagctcaaa cggcaattcc
      301 gccagaaagc gccgctgcgg cgcgctcagc cggccgcgcg tccactcgat cgcggcctgg
      361 gcttcggcat tcatcgattc cgccgtggcg ccgacggcgt tgtcgtggtt gccgcgcacg
      421 gccatggcgc catcatcgac gaggcccatc accgtctcga tggtccattc cggatcggcg
      481 ccatagccga cgtaatcgcc gaggcaaatc atccgttccg cgccacgcgc ccgcgcgaaa
      541 tcgaggcacg cgctgaatgc ctgccggttg gcgtggatat ccgcaaaaat ggccagcagc
      601 acgctttcct ccacacccat cctatcggcc gggcctgtac cggatttgat ggatatcaaa
      661 caacacagcg ccggctggcc gcaatcaggc tgacggcaag gaattgcggt catgcaggga
      721 gacaaaagcg cttctgcgat ccgccggacg cgccggcgat agcgcacggg agatatgatt
      781 tggagagatg attagcgctt gcgcggtgct tccaggcccc ggagcaacag cgccagcacg
      841 gcgtcgatcc gcgctgcgag ctgtgggtcg ctccattcct cggcatgcgc gggatgatgg
      901 aagcggctgg tcgcctcgaa aaccgcacgc gccgaggctt tgacgtcggc gacctggaac
      961 acgccctgct tgacgccgtc ggataaaata tgcgcgagtt ggtcgatcag accgtccttg
     1021 tgtgccttga ccaccttaca cgcctcttgc gccagcgtca gataggtccg gaacatctcg
     1081 ggatcgtcgc aaactttctt gtgcttgatc tcgatcatcg tgcgcagcca gcgctccagc
     1141 ctggccggcg caggccccga tccttccgcg atcttctgga gcggcgcgtt gacacggtcg
     1201 agccagcgct tggccaccgc ctcgcgcagc gaagccttgc tcggaaaatg acggtagacg
     1261 ctgccgtggc tgacatcgag cgcgcgggca acatccacca ccgtggcctt tgccagtcca
     1321 aagcggcgca gcacgtcctc ggtgacttcg agaattcgct caggcgtcaa gaccacattt
     1381 tcgttcatgc ggacaccgtc ggtttttcgc cgtcgcagat ggccatccgt gcggcaggaa
     1441 agcgcgtgcc ttcggcggcg tccgggatgg catcgatacc ctcggcctcc tcaacggact
     1501 cgccgcaatc cggcattccc ctgcaatcga gaccaacgca acccccggga ccgtcggcac
     1561 cgagttttcc aagggttttt gggctcgttg tcaggaccgg ccctttggcg gccttcaatc
     1621 tggcggcctg tgcctcaaga tgatgggcta cttgaaggtt gagccagcga ttaagttgtg
     1681 cggtcagata aagaatttcg acattcatgg ggagtcctct gcaagtcccg gacacgcggg
     1741 tgatcagcca acgcgcccaa gtgacgccaa cctggcgccg gcttcttgaa tagcatatct
     1801 gactgacaga ttacaatatc tgtcagtcaa tgtttcgtga tccggagact cgggccggtc
     1861 ccggttgttc gaagcgttcg ccggcggcag cggtttgtct caccggcgct gctctgctaa
     1921 agcgcggcgt aatccccatg agccacgagt cgtcgatgat ttcccgctac acccgcccgg
     1981 aaatggccgc catctgggag gcccagacgc gcttcaagat ctggttcgag atcgaggcgc
     2041 atgcggcgga tgcgctggcg gaactcggcg tcatccccaa agaggcggca agagcgatct
     2101 gggccaaggc ccgggatgcc atcttcgatg tcgcccggat cgacgagatc gagcgcgaga
     2161 ccaagcacga cgtgatcgcc ttcctcaccc atctggcgga aatcgtcggc cccgaggcgc
     2221 gtttcgtgca ccagggcatg acctcgtccg acgtgctcga tacctgcctc aatgtgcagc
     2281 tgacccgcgc cgcggatctg ttgatcgccg acgtcgacaa ggttttggtg gccctcaaga
     2341 agcgcgcctt cgagcacaag atgacgccga ccatcggccg ctcccacggc atccatgccg
     2401 aaccggtg
//
LOCUS       DASWGM010000460         7684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1843107,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000460 DASWGM010000000
VERSION     DASWGM010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843107"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..693)
                     /gene="tagF"
                     /locus_tag="VGA15_13290"
     CDS             complement(1..693)
                     /gene="tagF"
                     /locus_tag="VGA15_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HEX9588702.1"
                     /translation="MRCGLFGKLGAKRDFIALATPRSFLEAWEPWVQSSLSASRHQLG
                     DRWQQAFLTAPVWRFWLGAGICGTTAVGAIMSSLDGMGRYYPLTLHVIADAGAPISPP
                     DVDTLDEWFGVAESFLLSTLAHDAAFDDISLGLDGLAVPRILSRAAADTSIVSLGDDM
                     AAMVMAGTDFATTLSALRATSPEVYAAASFWWTAGGGDFPPLALSCRGLPDPYRYSTL
                     LTGDLGLAVHAR"
     gene            complement(702..4223)
                     /gene="tssM"
                     /locus_tag="VGA15_13295"
     CDS             complement(702..4223)
                     /gene="tssM"
                     /locus_tag="VGA15_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEX9588703.1"
                     /translation="MRPKQITQMILYGVGLGSLAAVVYFAGPFIAIGNWHPLENAIVR
                     NIVILLLVAGAVGVAGFSFFRRRKAATQIADGISGAEQTVNDEPVLKERMKDALTTLK
                     VASGNKSGYLYDLPWYVIIGPPGAGKTTALVNSGLKFPLSRGTAPAAVAGVGGTRYCD
                     WWFTEEAVLIDTAGRYTTQDSDAKSDKESWFSFLDLLKKSRPRQPINGVLVAISIEDI
                     LTMSRQDLAAHAEAIRMRLLELHQRLKVSFPVYALFTKVDLVAGFTEYFSYLNEAGRR
                     QVWGATFQTADKNRNLIGDIPNEFDLLLERLSEETLDRLQDEPAPQHRVQLFGFAAQM
                     ARLKPQIHNFLNQIFEPTRYHVNASLRGFYFTSGTQQGTPIDQLIGSLARTFGAEEVS
                     VGAYHGSGKSFFLTNLIMKVIIGEADWVSTDRGAVRRALLIKTAGLSLIGLCAIGLSV
                     AWLTSYKKNSDLIEQTVQVDGDYASVGAPLIKQTLIADHDLDKVLPLLHRLRNAPAGY
                     AARDVQVPLSERFGLSQHPRLLSASDAAYHTALERMFRPRLLYRLEEQLNARLSEPAF
                     VYEALKVYLMLGGQHPPDRGLIKSWMQRDWADNLYPGATNAEGRRLLEEHLAAMFDLE
                     TEQPPLVELDGRLIREAQNSLARLSVAQRAYEFLKSEAGTSTTGDWIVTRKGGPDVSV
                     IFESKAGQSLDNIRVPAFFTYNGFQQMFIAKLGGLSERISRDRWVLGDAGQQTAIGQQ
                     YDALAGELLNVYSNDFVITWRTALGNLRLKKLLADKPKYDALRALSAPTSPLRQILES
                     IRDETALTKERPKPTNAPAPSDALARPSPALFNTQDGSPGAGIEAQFKPFHSVLEGDS
                     TRRPIDSVVANLNDIAQNLTLIIENPQMTAQATTSLQTQVAALRNNASRMPPPFSDML
                     RGAAAEFEGSIAASTAGQLLQSLRDQVTPVCQQTITNRYPFVRGSNQEVPLGDFAKLF
                     SPNGIIDKFFAQFLAPYADTSRPDWAWRKDSPVGRSLSPDTLKQFQNAAYIRDAFFQT
                     GSSMPQVSLAIKPPRAAGPGLDIKTEIGGTIIASPSAPTVATAFSAPSPPSSTATSTV
                     QWPGPSARTAISVSSETGPPSVLERIGPWSLFRMLEAGSLTAKGETASASFIVAGNEL
                     NYEISTGSVRNPLNLAKLREFRCPSGI"
     gene            complement(4247..5464)
                     /gene="tssL"
                     /locus_tag="VGA15_13300"
     CDS             complement(4247..5464)
                     /gene="tssL"
                     /locus_tag="VGA15_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, long
                     form"
                     /protein_id="HEX9588704.1"
                     /translation="MLRVDELVAPNANPIMRAAGPLLQLLGRLRVALMRASFASLMEQ
                     VADAVKFFETDIRSAGISEHQANTAKYVLCATADDIVQHIPTEDRHVWTQYSMLSRFF
                     GERVGGVRFFEILDRLKADPLVNYPVLELQHACLALGFQGMHRASTNGLAGLQTIQRN
                     LYETLRRVRPKSDADLSPHWRGQELANRRQRARVPVWMVAAVIAALLTGGYLTLRALL
                     SARAENAAEVALALHPGDPIELKRRVIAPPPPPPPPPPPDRITQLQRIRNALAAEHLA
                     CVMTADQTGSFIIIRVCDLVLFEPGQAAVLDAFKPVAARVAATLDKEPGHIKIVGHTD
                     TSPIRTVRFPSNFELSVERAKAVAAVLKRGLADSSRVDIEGKGPDVPIASNATPEGRA
                     KNRRVEIFIERSE"
     gene            complement(5758..7095)
                     /gene="tssK"
                     /locus_tag="VGA15_13305"
     CDS             complement(5758..7095)
                     /gene="tssK"
                     /locus_tag="VGA15_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX9588705.1"
                     /translation="MSWYTKVAWSEGLFLRPHHLQQNDRYHEHLLESRVRTSTPYPWG
                     FSELEIDRDLTQQSKFALRRAAGIMPDGTPFSIPADSPLPPAIIVPEAAAGQIVWLLM
                     PIAAANTRETDDREAESAARYIIGAETFIDSSSALRVEEEIDLALPRLSFELRKTAKS
                     GYVGLGIARITEVRDKTIVFDEKYVPPVLLCHAHAVVEGWIDRAIGWIDNKLEELARY
                     AADPTAGGGLQSVEYLVLQLLNRHIAVLGHFRNSSYVHPERLFQEFLRLVGELATFAT
                     AERRARTYPHYDQDDLENVFGPVMRDLQDFLSAQLGRRAIRLEIIQLRPNAFSSPIRD
                     RSLFRNATLVLEVAARRPLVEIQNDFPHLFKVGPNTKMNEIVHANLPGVPLVHLPTPP
                     PQIRAITDHVYFYLDRKSPLWSEFSKAASIGMHFSGDWPELELHLWAILEDRQ"
     gene            complement(7331..>7684)
                     /locus_tag="VGA15_13310"
     CDS             complement(7331..>7684)
                     /locus_tag="VGA15_13310"
                     /inference="COORDINATES: protein motif:HMM:NF042526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein"
                     /protein_id="HEX9588706.1"
                     /translation="APTADDALRIMFGFQGPSYLDAERAFAQGFDDLKRHQIKTYAAM
                     QRAVTLLMNDVDPAAIEAASQSDRGLAGMVGSRKARLWDIYVARWQAHAQRHDNGILD
                     TFMDYFARCYDGESK"
BASE COUNT         1533 a         2292 c         2363 g         1496 t
ORIGIN      
        1 tcacctcgcg tggacggcta accccagatc tccggtcagc aacgtggaat agcggtatgg
       61 gtccggcaaa ccgcggcaac tcaatgccag cggaggaaaa tcgccgccgc cggctgtcca
      121 ccagaagctt gcggcagcat aaacctcggg gctggtggca cgaagggctg acagcgtcgt
      181 cgcgaaatca gttccggcca tcaccattgc ggccatgtcg tcaccgagtg acacgatgct
      241 cgtgtcggca gcggcgcgag acagaattcg tggaaccgca aggccgtcca gccccaacga
      301 gatgtcgtcg aaagctgcat cgtgcgcgag cgtcgagagc agaaagctct cggccacgcc
      361 gaaccactca tccagagtat cgacgtcggg cggcgaaatc ggagcgccgg catccgcgat
      421 cacgtgcaac gtgaggggat agtagcgccc catgccgtcg agcgacgaca tgatcgcacc
      481 cacagccgtg gttccgcata ttccagcgcc aagccagaac cgccagaccg gcgcggtaag
      541 aaaggcctgt tgccatcggt cacccagctg gtgacggctc gccgacagtg aggattgcac
      601 ccacggctcc caggcttcga ggaagcttcg cggcgtcgcc aacgcgatga agtcgcgctt
      661 cgcacccagc tttccaaaca gaccgcagcg catgatcgta ttcaaattcc actggggcag
      721 cggaactccc tgagcttcgc cagattgagc ggattgcgaa ccgacccggt cgagatctca
      781 taattcaatt cgttgccggc cacgataaaa ctggcgctcg cggtttcgcc ttttgcagtc
      841 agtgaaccgg cctcgagcat ccgaaacagc gaccagggac cgatcctctc gagtaccgag
      901 ggcggcccgg tttcgctgct gaccgagatt gcggtacgcg ccgacggacc gggccactgc
      961 accgtggacg tcgcagtgct tgaaggcggt gacggcgcgc tgaaggctgt cgctacggtc
     1021 ggagcgcttg ggcttgcgat gatggtaccg ccgatttctg tcttgatgtc gagcccgggg
     1081 ccagcggcgc gcggcggctt gatggccaac gatacctgcg gcatgctgct gccggtctga
     1141 aaaaacgcgt cacggatata ggcggcattt tgaaactgct tcagcgtgtc cggcgacaac
     1201 gaccggccga ctggactgtc ctttcgccac gcccagtccg gtcgcgacgt atccgcatat
     1261 ggcgcaagga actgggcgaa gaacttgtcg atgatgccgt tcgggctgaa cagcttggcg
     1321 aaatctccaa gcggaacttc ctgattgctg ccacgcacga aaggatagcg attggtgatc
     1381 gtctgctggc agaccggggt aacctgatca cgcaacgatt gcagcagttg tccggctgtc
     1441 gaggccgcta tgctgccttc gaactcggca gctgcgccgc gcaacatatc ggagaacggt
     1501 ggcggcatac gcgacgcatt gttgcgcagc gcggcgacct gcgtttgcag cgaagtggtc
     1561 gcctgcgcgg tcatttgcgg gttctctatg atcagcgtca ggttctgcgc gatgtcgttc
     1621 aggttggcca cgacggaatc aatcggccgg cgggtgctgt caccctccag caccgaatga
     1681 aagggtttga actgcgcttc gatcccggca cctggtgaac catcctgcgt attgaacagt
     1741 gccggtgacg gccgcgctag cgcatccgac ggcgcgggcg cattggtggg ctttggccgc
     1801 tccttggtga gcgcggtttc gtcacggatc gactccagaa tttggcgcag cggcgaagtc
     1861 ggtgctgaca gcgcacgcag cgcgtcgtat ttcggtttgt ccgccagcag ttttttcaac
     1921 cgtaggtttc ccagcgccgt acgccaggtg atgacaaaat cattggaata gacattcaag
     1981 agttcaccgg ccaaggcgtc atattgctgg ccgatagcgg tctgctgtcc ggcgtcgccg
     2041 agcacccagc ggtcgcggct tatccgttcc gacagtccac ccagcttcgc aataaacatc
     2101 tgctggaagc cgttataggt gaagaaggcc ggaacgcgga tgttgtcgag tgactgccct
     2161 gccttgcttt cgaagatgac tgacacgtcc ggtccgcctt tgcgtgtcac gatccagtcg
     2221 cccgttgtcg aggtgcccgc ttccgatttc agaaattcgt aggcccgttg cgccacgcta
     2281 agacgagcaa gcgaattctg cgcctcccga atcaggcggc catcgagttc gaccagtggc
     2341 ggctgctcgg tctccagatc gaacatcgcg gcgagatgtt cctcgagcag ccgacggcct
     2401 tccgcgttgg tcgcgcctgg atagagattg tcggcccagt cacgctgcat ccaggatttg
     2461 atcaatcccc gatccggcgg atgctgaccg ccgagcatga gataaacctt caatgcttca
     2521 tagacgaagg ccggctcact gagcctggcg ttgagctgct cctccagccg gtatagcaga
     2581 cgcggacgga acatgcgttc gagcgcggta tgatatgcag catccgacgc cgacaacagc
     2641 cgtgggtgct ggctgagccc gaagcgctcg gatagcggca cctgcacatc gcgagccgca
     2701 tagccagcgg gtgcattgcg caggcgatgc agcagcggca gaaccttatc gagatcatga
     2761 tcggcaatca gggtttgctt gatcagcggc gccccgaccg atgcataatc accgtcgacc
     2821 tgcacggtct gctcgatcag gtcggaattc ttcttgtaac tggtgagcca ggcgacgctc
     2881 agcccgattg cgcacaggcc aatcagcgac agccctgcag tcttgatgag caatgcgcgc
     2941 cgcacggcgc cgcgatccgt cgagacccaa tcggcttcgc cgatgatgac cttcatgatc
     3001 agattcgtga gaaagaaact tttgccggag ccgtgataag ccccgacgga gacttcctcc
     3061 gcgccgaagg ttcgcgccag cgatccgatc aattgatcga tcggcgtgcc ttgctgggtt
     3121 cccgaggtga agtagaatcc gcgcaagctg gcgttgacgt gatagcgggt tggttcgaag
     3181 atctgattga gaaaattgtg gatttgcggt ttcagccgcg ccatttgcgc cgcgaagccg
     3241 aacaactgga cgcgatgctg gggagcgggt tcgtcctgta gccgatctag ggtttcctcg
     3301 ctgaggcgct ccagcaggag atcgaattcg ttaggaatgt cgccaatcag attcctgttc
     3361 ttgtccgcag tttgaaacgt tgcgccccag acctgtcgcc ggccggcctc gttaaggtag
     3421 ctgaagtact cggtaaagcc cgcgaccaga tcgaccttgg tgaacagcgc ataaaccgga
     3481 aaactgactt tgagccgctg atgcagttcg agcaatcgca tgcggatcgc ctccgcgtgc
     3541 gctgccagat cctggcgcga catggtcaga atatcctcga tactgatggc gaccaggacg
     3601 ccattgatcg gctgtcgcgg ccgagatttt ttcaatagat cgaggaacga gaaccagctt
     3661 tccttgtcgc tcttggcgtc ggaatcctgc gtcgtatagc gtcccgccgt atcgatcaga
     3721 acggcctctt ccgtgaacca ccaatcgcag tagcgcgtgc cgccgacgcc cgcgaccgct
     3781 gcgggggccg tcccccgcga cagcggaaat ttaagaccgg aattgaccag cgccgtggtc
     3841 ttgcccgcgc cgggcggacc gatgatgaca taccatggca gatcatacag ataaccggac
     3901 ttgttgccgc tggcgacctt cagcgtcgta agcgcgtcct tcatgcgctc cttgagcacg
     3961 ggctcgtcat tgactgtctg ttcggcgccg ctgatgccat cggcgatctg cgtcgcagcc
     4021 ttgcgacgcc ggaagaaact gaagcctgcg actcctacgg cgccggcgac gagaagaaga
     4081 atgacaatgt tgcgaacgat cgcattttca agcggatgcc agttgccgat ggcgatgaac
     4141 gggccagcga agtagacgac agccgccagc gagccgagcc cgacgccgta taaaatcatt
     4201 tgggtaattt gctttggacg catgctgccc ctttatccgt tctcggctat tcacttcgtt
     4261 cgataaagat ttcgacgcgg cggttcttgg ctcgcccctc cggcgtcgcg ttgctggcga
     4321 tcggcacgtc tggccccttt ccttcgatgt cgacccggct tgaatcggcg aggccccgct
     4381 tgagcacggc agcgacggcc ttcgcgcgct cgaccgagag ttcgaaattg gaagggaaac
     4441 gcacggtacg gatgggcgaa gtgtcggtat gacctacgat cttgatgtgc cccggttcct
     4501 tgtccagcgt cgctgcgacg cgggccgcga ccggcttgaa ggcgtcaagc acggcggcct
     4561 gtccgggctc gaagaggacg agatcgcaga cccggatgat gatgaagctc ccggtctgat
     4621 ccgcggtcat gacacaggcg agatgttccg ccgccagcgc gttgcgaatc cgctgcagct
     4681 gagtaatgcg atcgggaggt ggcggcggcg gaggcggcgg aggcggcgcg atgacgcgac
     4741 gcttcaactc gatgggatcc ccgggatgca gcgcaagcgc cacctcggcg gcgttttcgg
     4801 cccgggctga cagtagcgcc cgcagcgtga gataacctcc cgtcagcagc gccgcgatga
     4861 ccgctgcgac catccacacg ggcacgcgcg cgcgctgccg acgattggcc agttcctgac
     4921 cgcgccagtg cggcgacaga tctgcgtcgg atttcgggcg aacccggcgc aacgtttcgt
     4981 aaaggttgcg ctgaatcgtc tgcagtccgg cgagaccgtt tgtcgacgca cggtgcatac
     5041 cctggaagcc gagtgccagg cacgcgtgct gcaactcgag aaccgggtag ttgaccagcg
     5101 gatcggcttt gagccgatca aggatttcga aaaaccgcac accgccgacg cgttcgccga
     5161 agaaccggct cagcatcgaa tattgcgtcc agacatgacg gtcttcagtc ggaatatgct
     5221 gcacgatgtc gtcggccgtc gcgcacagaa cgtattttgc ggtgttggcc tgatgttcgg
     5281 agatccccgc cgatcgaata tcggtctcga aaaattttac ggcatcggcg acctgctcca
     5341 tcaggcttgc aaacgatgcg cgcatcaggg cgacgcgcag gcggccgagc agttgcagca
     5401 gcggcccggc ggcgcgcatg atcggattgg cattcggcgc caccagttcg tcgacgcgca
     5461 gcatcggtgc ggctggctcc gcgaaccgcg gctgctgcgg tattgtcggt gtggatatcc
     5521 agtcttcggg cgcgtggctc gtgggcggag gcgcatagcc gggcgattgc ggtgaatatg
     5581 ctgaggacgg gctcgtcgaa ggaggcgtcg gcgaatacgg cgcacgcggc ggcgcccgcc
     5641 caggatcggc agactgcgga taaggatcag gagccggcgg cagccttcca cccggattag
     5701 gacgcatgat cgtccgctcg actcgaccga acggatttgc cggcttgtcc ttgtcgttca
     5761 ttggcggtct tccagaatcg cccacaaatg cagttcgagt tccggccagt cgcccgagaa
     5821 gtgcatcccg atcgatgccg ccttgctgaa ctccgaccac agcggcgact tacgatccag
     5881 ataaaagtac acgtgatcgg tgatcgcacg aatttgaggc ggcggcgtcg gcagatgcac
     5941 cagcggcacg cccggcaggt tggcatgcac gatctcgttc atcttggtgt tgggcccgac
     6001 cttgaacagg tgcggaaagt cgttttggat ttcgaccagc ggacggcgcg ccgcaacttc
     6061 gaggaccagc gtcgcattgc ggaacagcga cctgtcgcga atgggcgatg aaaacgcgtt
     6121 gggccgcaac tggatgatct cgagccggat tgcgcgtcgg ccgagctgcg cactcagaaa
     6181 atcctgcagg tcccgcatca ccggtccgaa aacgttttcc aggtcatcct gatcgtagtg
     6241 gggatacgtg cgggcccgcc gctcggccgt tgcgaaagtg gcgagttcgc cgaccagcct
     6301 caaaaattcc tggaacaggc gctcgggatg gacgtagctg gaattccgga aatgcccgag
     6361 caccgcaatg tgccgattca gcaattgcag gaccaaatac tcaacgctct gcagtccgcc
     6421 acctgcggtt ggatccgccg catagcgcgc aagctcttca agtttgttat cgatccagcc
     6481 gatcgcacga tcgatccaac cctccacgac agcatgggca tgacacagca ggaccggagg
     6541 aacatatttt tcatcaaaaa caatcgtttt gtctcgcact tcagtaattc tggcgatgcc
     6601 aaggccgacg tagccggact tagcagtctt gcgcagttcg aagctgaggc gcggaagggc
     6661 aaggtcgatt tcctcctcga cgcgtagtgc cgaggaggaa tcgatgaagg tttcggcgcc
     6721 gatgatgtag cgtgccgcgc tttccgcctc gcggtcatcc gtttcacgcg tgttggcggc
     6781 ggcgatcggc atcaagagcc agacgatctg gccggccgcc gcctccggca cgatgattgc
     6841 gggaggcagc ggactatcgg ccgggatgct gaaaggggtg ccatcaggca tgatgccagc
     6901 agctcgccgc agcgcgaatt tgctttgctg ggtcagatcg cgatcaatct cgagctcgga
     6961 gaagccccat ggatacggcg tcgaagtccg cacccgggat tcgagcaggt gctcatgata
     7021 gcggtcattc tgctgcagat gatggggccg aaggaagaga ccttccgacc acgctacttt
     7081 tgtgtaccag gacattatcg gatctctcga atagccggat agagcccccg agcgcgccag
     7141 cctttcccgg atttattcgg ctgactccaa tcttggtttc gctcaatccg attttggttc
     7201 aaatgagaac aggatctccg cctgtgaaga acggcaagca acgctccgac cttacccaat
     7261 gtgtatctta cccaatgtgt attatgaggc tcttcggcaa taaaactgaa gctatacaga
     7321 caacgcccga tcattttgat tcaccatcgt agcatcgcgc aaagtagtcc atgaacgtgt
     7381 cgagtatccc gttatcatgc cgctgcgcat gggcctgcca acgagccacg tagatgtccc
     7441 acaaccgcgc cttgcgcgaa cccaccatac ccgcaagacc acgatcggat tgggatgcgg
     7501 cttcgatcgc tgcagggtct acgtcgttca tcaacagcgt gacggcccgc tgcatcgccg
     7561 cataggtttt gatttgatgc ctcttcagat catcaaagcc ttgggcgaac gctctctcgg
     7621 cgtcgagata actcgggccc tggaagccaa acatgatgcg aagggcgtca tccgcggttg
     7681 gcgc
//
LOCUS       DASWGM010000461         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_585422,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000461 DASWGM010000000
VERSION     DASWGM010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585422"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VGA15_13315"
     CDS             <1..145
                     /locus_tag="VGA15_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9588707.1"
                     /translation="QAMMVLQIQLTKRQGIVPMTRDYVAGEEARLRGLEGGQRPPLRL
                     AGE"
     gene            334..1830
                     /locus_tag="VGA15_13320"
     CDS             334..1830
                     /locus_tag="VGA15_13320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9588708.1"
                     /translation="MAPQDATARTLASPATPLYRTAPHNIEAEQALLGAILINNDAFY
                     RVSDFLKPAHFFETVHQKIFDIAANLIRVGKTATPITLKTFLPPDLDIAGLSVSQYLA
                     RLAAEATTVINAEDYGRTIYDLAIRRDLIRIGDDMVNVAYEAPVEFSPQNQIEDAERR
                     LYELAELGQYGSGFLRFIDAMTTAVDMAANAYRREGGLSGIATGLKDVDQYMGGLQKS
                     DLIIVAGRPGMGKTSLATNIAYNVARAFEPRTLSDGTIEAANGGRVGFFSLEMSAEQL
                     ATRIISEQTDIPSSTIRRGQITENDFDRIVAFTRTMQQLPLYIDETGGLSIAQLAARA
                     RRLKRQRGLDFLVIDYLQLLQGSTRRANEGRVQEVTEITTGLKALAKELELPILALSQ
                     LSRQVENRDDKRPQLSDLRESGSIEQDADVVLFLFREEYYLKNKEPRAGTEEHFKWQA
                     EMDAVHGKAEVIIGKQRHGPTGTIQLQFKADVTRFSNLAQEERLPSHF"
BASE COUNT          405 a          635 c          607 g          342 t
ORIGIN      
        1 gcaggccatg atggtgctgc agatccagct gaccaagcgt cagggcatcg tgcccatgac
       61 gcgcgactat gtcgccggcg aggaggcgag gctgcgcggg ctcgaaggcg ggcagcgccc
      121 gccgctgcgg ctcgccgggg aatgagtcat tctgccgcag tccgaccctt ccgccatctt
      181 ggacaacgtt ggacgcgaat caaagtgaat tgctaaaaac taattcggcg ctgtggattg
      241 ctttgataac gccaagaatt cggcccaacg ctcacctgct cccgagttgt ccaccgggag
      301 ccgtgctacg gacgccgtca gcgaggaaac cgaatggctc cccaagacgc gacggcacgc
      361 accctggctt ccccggcgac gccgctgtac cgcaccgcac cgcacaatat cgaagcggag
      421 caggcgctgc tgggtgcgat tctcatcaac aacgacgcgt tctaccgggt ctcggacttc
      481 ctgaagcctg cgcatttctt cgagacggtg catcagaaga tcttcgacat cgccgcaaac
      541 ctcatccggg tgggaaagac cgcgactccg atcacgctga agacctttct gcctccggat
      601 ctggatatcg ccggtttgag cgtcagccag tacctcgcac ggctcgccgc ggaggccacg
      661 accgtcatca atgccgagga ttatggccgc acgatctacg atctggcgat ccggcgggac
      721 ttgatccgca tcggcgacga tatggtcaac gtcgcctatg aggcacccgt cgagttttct
      781 ccgcaaaatc agatcgagga cgccgagcgc agattgtacg aattggcgga gctcggacag
      841 tacggcagcg gcttcctacg gtttatcgac gcgatgacca cggccgtcga catggccgcc
      901 aatgcctacc gcagggaggg cggactctcc gggatcgcga ccggattgaa ggatgtcgac
      961 caatacatgg gcgggctgca gaaatcggat ctgatcatcg tcgccggccg cccgggcatg
     1021 ggcaagacgt cgctcgccac caacatcgcc tacaacgtcg ctcgcgcgtt cgaaccgcgc
     1081 acgctgtcgg acggcacgat cgaggccgcg aacggcggca gagtgggatt cttctcgctc
     1141 gaaatgtccg ccgagcagct cgcgacccga atcatttccg agcagacgga catcccctct
     1201 tctactattc ggcgcggtca gatcacggag aacgatttcg acaggatcgt cgccttcaca
     1261 cggaccatgc agcaattgcc gctgtacatc gatgaaaccg gcggcctctc gatcgcgcaa
     1321 ttggcggcgc gcgcccgccg cctcaaacgc cagcgcggcc tggattttct cgtgatcgat
     1381 tatctgcagc tgttgcaagg ctcgacccgg cgcgccaacg agggccgggt gcaggaggtg
     1441 accgagatca ccaccgggct gaaggcgctg gccaaggagc tcgagctgcc gatcctcgcg
     1501 ctgtcccagc tctcccgcca ggtcgagaac cgggacgaca agcgcccgca gctatcggac
     1561 ctgcgagagt ccggctcgat cgagcaggac gccgacgtcg tgctgttcct gttccgcgag
     1621 gaatattacc tgaagaacaa ggagccacgt gccggcaccg aagaacattt caagtggcag
     1681 gcggaaatgg acgcggtcca tggcaaggcc gaagtcatca tcggcaagca gcgtcacgga
     1741 cccaccggaa cgatccagct gcagttcaag gccgacgtga cccgcttctc caatcttgcg
     1801 caggaggaac ggctgcccag ccatttctga tttcgtaaat tccttccacg aaggaatcgg
     1861 ctcgggcgtg gggcggaagc gcgacccaat tccacggcga cgcccgattg agccctgcga
     1921 cgttttcctg tctcatcgcc ggcggaccga tgtggtctcg ccatgggcga gggatgggaa
     1981 tgaccgagc
//
LOCUS       DASWGM010000462         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2407420,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000462 DASWGM010000000
VERSION     DASWGM010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2407420"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VGA15_13325"
     CDS             <1..363
                     /locus_tag="VGA15_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9588709.1"
                     /translation="VEALNGPQDFIPVNNKMFKERRDLVVSMLNQARGIDCPRPQGAF
                     YVYPSCAGTIGKSSPSGKVIANDEDFVTELLETEGVAVVQGSAFGLGPAFRISYAAKT
                     SDLEDACKRIQRFCGNLR"
     gene            484..1005
                     /locus_tag="VGA15_13330"
     CDS             484..1005
                     /locus_tag="VGA15_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HEX9588710.1"
                     /translation="MRLSTLSIAIVALVAPIASANALPPVRAGILQCQGGQNVGFVVG
                     SVTSLECVFQSEGRRPEPYIATVRRYGLDIGFTEQTKFSWAVNAPTGRVGRGDLAGNY
                     GGVGANASVGVGGGGNFLVGGQQNSYALQPLSVQGQTGLNVAAGVADIELQPVRFGHG
                     GPRGHTRRHHRHG"
     gene            1331..1822
                     /locus_tag="VGA15_13335"
     CDS             1331..1822
                     /locus_tag="VGA15_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HEX9588711.1"
                     /translation="MRRSFILAGVAITLTMLVALFAAAHAQQPMQRVQVGVLECRGGA
                     SIGFIVGSVTNLGCVLRVEGMPEDRYVATIRKVGLDLGITQESALAWGVYAPVARLGP
                     GDLSGNYAGAQGSASVGVGIGGNALVGGSANSIALQPLSVQGQIGLNVAAGLESLELR
                     PGR"
BASE COUNT          355 a          649 c          675 g          416 t
ORIGIN      
        1 gtcgaagcgc tcaacggtcc gcaggacttc atccccgtga acaacaagat gttcaaggaa
       61 cgccgcgatc tggtggtgtc gatgctcaat caggccaggg gtatcgattg tccgcggccg
      121 cagggcgcgt tctatgtcta cccgtcctgc gccggcacca tcggcaagtc gtcgccgtcg
      181 ggcaaggtca tcgccaacga cgaggacttc gtcaccgaac tcttggagac cgaaggcgtc
      241 gcggtggtgc agggttcggc gttcgggctc ggcccggcgt ttcggatctc ctacgcggcc
      301 aagacctccg atctcgaaga tgcctgcaaa cgcatccagc gattttgcgg caatttgcga
      361 tagtcaaatc atttcgctat tccgcatttg atattttgac ggcgggtttt gcgccttgtt
      421 ttggacacgg cgcttccttc ctgtccgctc cgcgaattca actcatctgc ggggaaatga
      481 ctcatgcgac tctcgacact tagtattgcc atcgttgcgc tggttgcgcc gatcgcaagc
      541 gccaatgcgc tgccgccggt tcgcgccggt atcctgcaat gccagggtgg ccagaatgtc
      601 ggcttcgtcg tcggctcggt gaccagcctc gaatgtgtat tccagagcga aggccgccgt
      661 cccgaaccct atatcgcaac cgtccgccgc tatggtctgg atatcggctt caccgagcag
      721 accaagtttt cctgggcggt caatgcgccg accggccggg tgggacgcgg cgatctcgcg
      781 ggcaattacg gtggcgtcgg cgccaacgcg tcggtcgggg tcggcggcgg cggcaatttt
      841 ctggtcggcg gccagcaaaa ttcctacgcg ctgcaacccc tcagtgtgca aggccagacc
      901 gggttgaacg tcgccgccgg ggttgccgat atcgaactgc agccggtccg gtttgggcac
      961 ggcggtcctc gcgggcacac tcgccggcat caccgccacg gttgatttaa caacggattt
     1021 tacgaaaaga ggctcgcgaa agcgggcctc tttttatttg gtacaatgtc attccggggc
     1081 gcgccaacgg ggtcgcgcga atgggcgccc gatgacagcc tccgtgcgaa cccggaatct
     1141 cgagattccg ggttcatcgc tccgcgatgc cccggaatga cagtctgata gatcagcgtc
     1201 gccgcaaaag cccgcccggc aaatctggca cggccgcgga tgttgtgcca tagaaaagca
     1261 agcgccgcgc ctatcggcgc tctcctgatt ctctgctcgc atcacatctt ttcagccgga
     1321 gattttcttt atgcgccgtt cattcatcct tgccggggtc gccattaccc tcaccatgct
     1381 ggttgcgctg ttcgccgccg cccatgcgca gcagccgatg cagcgcgtac aggtcggcgt
     1441 gctcgagtgc cgcggcggtg caagcatcgg cttcatcgtc ggctcggtga ccaatctcgg
     1501 ttgcgtgctg cgcgtcgagg gcatgccgga ggaccgctac gtcgcgacca tccgcaaggt
     1561 cggtctcgat ctcggcatca cccaggaatc ggcgctggcc tggggcgtgt acgcgccggt
     1621 ggcgcggctc gggcccggcg atctatccgg caactacgcc ggcgcgcagg gcagcgcgtc
     1681 ggtcggcgtc gggatcggcg gcaacgcgct ggtgggggga tcggcgaatt cgatcgcgct
     1741 gcagccgctc agcgtgcagg gacagatcgg cctcaatgtc gcggccgggc tggaaagcct
     1801 ggaacttcgg ccgggaaggt aaggctgcgt tccccggatg cagcgcagca cgaagtgttg
     1861 cgctgaatcc ggggtccatc tcattggcga atgggtgccg gctctgcggt gcatcgtgga
     1921 gaacgctgca ccgtgtccgg gacacgatgc cttccagcga cgtttttccg gcttgccaaa
     1981 tcgtcggcgt agggggagca tccagcctgc cgacccaatc aagggccgag ctccagatga
     2041 ggaggcggca atgggacagg acgtcagaag tcctcgcggt ccacggtgca ttgcg
//
LOCUS       DASWGM010000463         8725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1148589,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000463 DASWGM010000000
VERSION     DASWGM010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148589"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1459
                     /locus_tag="VGA15_13340"
     CDS             77..1459
                     /locus_tag="VGA15_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HEX9588712.1"
                     /translation="MSRIVELLCSRRLPHVAVLALMSIGFAGCSGDMQTRLSQDSLSN
                     PFASDATGMVPPAPVERRELSRNPRPQPQYQSQYQSQALPPPIAAPQSNPTASAGISG
                     GGRGLASYAPPARPPLETTGTVAPRSVAASHGGGQAGQGPTTIIVGTSDTLDTLSRRY
                     NVSPTAILQANGYKGPRTLSPGQQLIIPHPTAVVAAPAVAPAPTLVAAASRAVAVAAA
                     PTVHVVNRGDTLLSIARRNHVPVAELARANHLEPSTKIRLGMKLTVPGAKTAAVAPAP
                     QPGMAAVGFQPGAAVAAPATKMAAAGGAPQSARLAQATATVEDAADATPAKAAEATGA
                     LPTFRWPVRGKVITSYGAKTNGKSNDGINLAVPEGTPVKAAEDGVVAYSGNELKGYGN
                     LVLVRHSNGYVTAYAHASELLVKRGDTIKRGQIIAKSGQSGEVGSPQLHFEIRKGSSP
                     VDPLQFLNGA"
     gene            complement(1575..2543)
                     /locus_tag="VGA15_13345"
     CDS             complement(1575..2543)
                     /locus_tag="VGA15_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9588713.1"
                     /translation="MSKKPGKTRPQVASRASSRKLPMAAAKRPAVPSKHDTADRIADA
                     LEAIAAHLSAAAPMPNSAETFGGADAFVWHPDGRLAPVPRVSRVDLGLLKGIEQVRDI
                     LIENTERFADGLPANNALLWGARGMGKSSLVKAAHASINVDRKPAERLKLIEIHREDI
                     ESLPALMDLLRSSDFRFIVFCDDLSFDGNDASYKSLKAALEGGIEGRPDNVILYATSN
                     RRHLLAREMIENERSTAINPGEAVEEKVSLSDRFGLWLGFHRCSQDEYLAMVKGYCGH
                     FGIELAEDELDREALEWSTTRGSRSGRVAWQFTQELAGRLGVRLRP"
     gene            2742..3107
                     /gene="yajC"
                     /locus_tag="VGA15_13350"
     CDS             2742..3107
                     /gene="yajC"
                     /locus_tag="VGA15_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX9588714.1"
                     /translation="MFITPAFAQAAGAGDTNSMLMSLLPFALIFVIMYFLILRPQQKK
                     VKDHAELVKNIRRGDTVVTSGGLVGKVTKVVDDDQIEFEISDGVRVRQMRQMISGVRA
                     KGEPAKEKSEPAKEDTPAS"
     gene            3156..4757
                     /gene="secD"
                     /locus_tag="VGA15_13355"
     CDS             3156..4757
                     /gene="secD"
                     /locus_tag="VGA15_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX9588715.1"
                     /translation="MLYFTRWKALAIILTALAVCLCAVPNFFPEATVKTWPQWAQRHL
                     VLGLDLQGGSHLLLEVDANAVKKDKLDQVRDDVRRVLRENKIVYTALASRADSVEVRI
                     TKDSDLPTALSKLRELSQPLGGLLGSSGQRSLDVSDAGSGLIRLTVPQAAVTDRIRQA
                     VEQSIQIVERRVNELGTVEPLIQRQGNDRILVQVPGLGDPSRLIAILGQTAKMDFRMV
                     DSTVSPDQALAGRVPPDSEVLPSAEASRQPYVIKKQVLVSGGDLTDAQPGFDQRSGEP
                     IVSFRFNTSGSRKFAQATSENVGQPFAIVLDNKVISAPVIREPITGGSGQISGNFTVQ
                     AANDLAILLRAGALPAPLTVIEQRTVGPGLGQDSIQKGELAAYVGSIMVIVFMLVTYR
                     LFGVFANIAVAINVAMIFGVLSLLNATLTLPGIAGIVLTVGIAVDSNVLIYERIREEI
                     RGGRTAISAIDAGFKRALSTILDSNITTFIAAAVLFYIGTGPVRGFAVTLGIGIITTV
                     FTAFELTRLIVAAWVMWKRPSSVPI"
     gene            4873..5877
                     /gene="secF"
                     /locus_tag="VGA15_13360"
     CDS             4873..5877
                     /gene="secF"
                     /locus_tag="VGA15_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX9588716.1"
                     /translation="MTHLVLIGLGILIAVLTVVSCFGLLPSLRIVPDDTKFDFTRFRR
                     ISFPISALLSIVAITLFFTHGLNFGIDFKGGTLMEVRAKSGAADLASMRSTLSTLGVG
                     DVQLQQFGGPADVLIRVAEQPGGDAAQQAAVQKVRGALGDAVDYRRVEVVGPRVSGEL
                     LAYGMLGLMLAIFAILIYLWFRFEWQFALGAMIANVHDIVLTIGFMSITQVDFDLTSI
                     AALLTILGYSLNDTVVIYDRIREMLRRYKKMPMPELLNESINSTLSRSIITHVTVTLA
                     LLALLLFGGHAIHSFTAVMMFGVVLVGTYTSIFIAAPILIYLGVGTNRADAPDTPKKK
                     "
     gene            5893..6276
                     /locus_tag="VGA15_13365"
     CDS             5893..6276
                     /locus_tag="VGA15_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX9588717.1"
                     /translation="MAVPPDAPHLPRSAPIEAYGRYGFAFADMSHRGSLLCLPEAIWA
                     WPVTRPEDIDEASLARVFAAANGIDTLIIGTGTDVWLPPPALREALRAVRVVLDAMQT
                     GPAIRTYNIMIGERRRVAAALIAVP"
     gene            6273..7142
                     /locus_tag="VGA15_13370"
     CDS             6273..7142
                     /locus_tag="VGA15_13370"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEX9588718.1"
                     /translation="MSGADGLADSADFCAQLVRTNDFARYASTLFVPAGERRALLAIY
                     AFNVEISRVREQVSQPLPGEMRLQWWTDMLAGEGHGGIEGNPVAAELQLAIQDWQLPV
                     ERLSRLIDEHQFDLYNDPMPTMAALESYLNDTASALFSLGAGIAGGQSGEIEHLARHA
                     GLAQGIAQVMAALPRDASRRQLFVPLQVLQQHGSGMEEVFAGKQTPKLRAVLDQLVGD
                     AQGHLKTAFALLATVPPQVRPVFLPLKLVARELMRMSRADSDPFAPWVRSRLRTLWTL
                     WRASRSREFRDGG"
     gene            complement(7314..8417)
                     /locus_tag="VGA15_13375"
     CDS             complement(7314..8417)
                     /locus_tag="VGA15_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX9588719.1"
                     /translation="MSLPRDDAAVLSARWIEGVLLKRDVFSTVERGRFRSEAGEVDAV
                     LRRLDAVPWWSYIPARYLFARERRALALARDLHVGPELLWAGRQALVRGFINGVALHL
                     AKPTGDVAYFRSAKQALRKLHRAGICHNDLAKEQNWLRGSDGNAYVTDFQLAACFKTR
                     GRLFRIAAYEDLRHLLKHKRSYAPQALTPKERKILARKSLVASIWLLTGKKVYRAITR
                     GLFNFTDREGGGRRLVNDAPVLIDLIRKNPGVRDTAIVAFADRRTGVGLYAFVEADQT
                     ALEAQLRNGLNAAKGPKPPEHIQVVQALPRDAAGKVRTEMLQLVAMNQLDLIEPMMTS
                     EADRAFLKSILESRKNLRDRFNFEAADLAPPSS"
     gene            complement(8425..>8725)
                     /locus_tag="VGA15_13380"
     CDS             complement(8425..>8725)
                     /locus_tag="VGA15_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEX9588720.1"
                     /translation="DARSTSLAPPPATTALGSLLSHITGGHIETIDPVARSFQPMNIN
                     FGLFPPLASPPTKKPEGTRLRGNDKTIAKKQAMSARALSDLDRWIADSLHVAAAA"
BASE COUNT         1515 a         2767 c         2788 g         1655 t
ORIGIN      
        1 tccgaagggt taagcggttg tttactgggg gtgtgtttac tcaaaacagt atcaagttgc
       61 gtacgagtga gtaaccatgt cccgaatcgt cgagttgctt tgctcgcgcc gcttgccgca
      121 cgtcgcggtg ctggcgctga tgtcgatcgg ttttgccggc tgcagcggcg acatgcagac
      181 gcggctctcg caagattcct tgtccaatcc ctttgcctcg gatgccaccg gcatggtgcc
      241 gccagccccg gtcgagcgcc gcgaactatc gcgaaatccc cgcccgcaac cgcaatacca
      301 gtcgcaatac cagtcacagg ccctgccgcc gccgatcgca gcaccgcagt ctaatccgac
      361 ggccagtgcc ggcatttccg gaggagggcg cgggctggct tcctatgcgc cgcccgcccg
      421 cccgccgctt gaaaccaccg gcaccgtcgc cccgcgctcg gtagcggcaa gccacggcgg
      481 aggccaagca gggcagggcc ccaccacgat catcgtcggc accagtgaca ccctcgatac
      541 gctgtcgcgc cgctacaatg tttcgccgac cgcgatcctg caggccaacg gttacaaggg
      601 gccgcgcacg ctgtcgccgg gccagcaact gatcattccg catccgaccg cagtggttgc
      661 tgcacctgcc gttgcacctg cacccacact ggtagcggcg gcgagccggg ccgttgcggt
      721 ggccgcggcg ccgaccgttc atgtcgtcaa tcgcggcgat accttactca gcatcgcgcg
      781 tcgcaatcac gtgccggtcg ccgaactcgc aagagccaac catctcgaac cgtcaaccaa
      841 aatcaggctt ggcatgaagc tcaccgtgcc cggtgcaaag accgcagccg tcgcaccggc
      901 gccgcagccg ggaatggcgg ctgttgggtt tcagcccggc gctgccgtgg cggcgcccgc
      961 taccaaaatg gctgcggcag gcggtgcccc gcaaagcgcg cggctcgccc aggccacggc
     1021 aacggtcgaa gacgccgcgg atgcaacgcc ggccaaggcc gccgaggcca ccggcgcgct
     1081 gccgacattc cgctggccgg tgcgcggcaa ggtgatcacg agctatggcg ccaagaccaa
     1141 tggcaagtcg aatgacggca tcaatttggc ggtgccggaa ggcacgccgg tgaaggccgc
     1201 ggaggacggc gtcgtcgcct attccggaaa tgagctcaag ggttatggca atctggttct
     1261 ggttcggcac tccaacggtt acgtcaccgc atatgcccat gcgagtgaac tgctggtgaa
     1321 gcgcggggac acgatcaagc gcggccagat cattgccaaa tcgggtcagt cgggcgaggt
     1381 ggggtcgccg cagcttcact tcgagatccg caagggatct tcaccggttg acccgcttca
     1441 attcctgaac ggggcgtgag cggtcaggta gtttactgat cgtcattgcc ggacttgatc
     1501 cggcaatcca tcgtcgagaa aagcgttttt tgcgaagatt gatggatacg cgggtcaagc
     1561 ccgcgtatga ccgtttacgg cctaagcctt acccccagcc gccctgccag ttcctgcgtg
     1621 aactgccagg cgacgcggcc cgaacgcgag ccgcgcgtgg tcgaccattc cagcgcttcg
     1681 cgatcgagct cgtcttccgc gagctcgatg ccgaagtgac cgcaatagcc tttcaccatc
     1741 gcaaggtact catcctggct gcagcgatga aagccgagcc atagtccgaa acggtccgac
     1801 agcgatacct tctcctcgac cgcttcgccg ggattgatgg cggtcgagcg ttcgttctcg
     1861 atcatctcgc gggccaaaag atgccggcgg ttcgaggtgg cgtacaggat gacattgtcg
     1921 ggccggcctt cgatgccacc ctccagcgcc gccttcagcg atttgtagga ggcatcgttg
     1981 ccgtcgaacg acaggtcgtc gcagaacacg atgaaacgga aatccgaact gcgcagcagg
     2041 tccatcagcg ccggcaggct ctcgatatcc tcgcgatgaa tctcgatcag tttcaagcgc
     2101 tcggcgggtt tgcggtcgac gttgatgctg gcatgcgcgg ccttcaccag cgacgatttg
     2161 cccatgccgc gcgcgcccca cagcagcgcg ttgttggcgg gcaggccgtc ggcgaagcgt
     2221 tcggtattct cgatcaggat atcgcggacc tgctcgatcc ccttgagcag gccgagatcg
     2281 acgcggctga cgcgcggcac cggcgccagc cgcccgtcgg ggtgccagac gaatgcgtcg
     2341 gcgccgccaa atgtctcggc cgaattgggc atcggtgctg ccgccgacag gtgcgctgca
     2401 atggcctcga gcgcgtccgc aatcctgtcc gcggtgtcat gtttggaagg cactgccgga
     2461 cgttttgccg cggccatcgg caatttgcgc gagctcgccc tggaggcgac ctgaggtctg
     2521 gttttgccgg gttttttcga catcttgcgg gttcctgacc acgcagccat atcgggccaa
     2581 atggccgacc gcaagcggcc caaatgaggg gtctggcggc gttgcaattg ggaccgcggc
     2641 cgctatagtc cgcgcgaatt tgaccggacc ggcccgaccc ccgatgcgtc gccggttcat
     2701 ggccgtttca cgaggaatgg ccgtttcacg aggatgtccg aatgttcata actcccgcgt
     2761 ttgcccaggc tgcgggcgcc ggtgatacca acagcatgtt gatgtcgctg ctgccgttcg
     2821 cgctgatctt cgtgatcatg tatttcctga ttctgcggcc gcagcagaag aaggtgaagg
     2881 accacgccga actcgtgaaa aacatccgcc gcggcgatac cgtggtgacg tcaggcggcc
     2941 tcgtcggcaa agtgaccaaa gtggtcgacg acgaccagat cgagttcgaa atctccgatg
     3001 gggtgcgcgt gcgccagatg cggcagatga tttccggcgt gcgcgccaag ggcgagccgg
     3061 ccaaggaaaa gagtgagccc gccaaggaag atactccggc gagctagcgc gttttctgat
     3121 cgcgcccatt ccttaagatc tgacgggtca actcgatgtt gtatttcacg cggtggaagg
     3181 cgctggcgat catcctgacg gcgctggcgg tgtgcctttg cgccgttccg aatttcttcc
     3241 ccgaagcgac ggtgaaaacc tggccgcaat gggcgcagcg ccatctcgtg ctcggcctcg
     3301 acctgcaggg cggttcgcac cttctgctcg aggtcgatgc caatgcggtc aagaaagaca
     3361 agctcgatca ggtgcgtgat gatgtccgcc gcgtcctgcg tgaaaacaag atcgtctaca
     3421 ccgcgcttgc atctcgcgcc gacagcgtcg aagtgcggat caccaaggac agcgacctgc
     3481 cgaccgcgct gagcaagctg cgcgagttgt cgcaaccgct cggaggtttg ctcggttcca
     3541 gcggccagcg cagcctcgac gtcagcgacg ccggtagcgg attgatccgg ctcaccgtcc
     3601 cgcaggcggc ggtcaccgac cgcatcaggc aagccgtcga acagtcgatc cagatcgtcg
     3661 agcgccgcgt caatgaactc ggcacggtcg aacccctgat tcaaagacag ggcaatgatc
     3721 gcattctggt gcaagtgccg ggactgggcg atccgtctcg cctgatcgcc attctcgggc
     3781 agaccgccaa gatggatttc cggatggtcg attcgaccgt ttcgccggat caggccctcg
     3841 cggggcgggt gccgccggat tcggaagtcc tgccaagcgc cgaggcgtcc cgccaaccct
     3901 acgtcatcaa aaagcaggtg ctggtctccg gcggcgacct gaccgacgcg cagcccggtt
     3961 tcgatcaacg ctcgggcgag ccgatcgtaa gcttccgctt caacacctcc ggttcacgca
     4021 aattcgcgca ggccacgtcg gaaaatgtcg ggcaaccctt tgccatcgtg ctcgacaaca
     4081 aggtcatttc ggcacccgtc atccgcgaac cgatcaccgg cggctccggc cagatttcgg
     4141 gtaattttac ggtgcaggcg gccaatgatc tcgcgatcct cttgcgtgcc ggcgcgctgc
     4201 cggcgccgtt gacggtcatt gaacagcgaa ccgtcggacc ggggctcggt caggattcga
     4261 tccagaaggg tgaactcgcc gcttacgtcg gctcgatcat ggtgatcgtg ttcatgctgg
     4321 tgacctaccg gctgttcggc gtgttcgcca acatcgcggt cgcgatcaat gtcgcgatga
     4381 tcttcggcgt attgtcgcta ttgaatgcga cgctgacgct gcccggcatc gccggcatcg
     4441 tgctcacggt cggcattgcc gtcgactcca acgtgctgat ctatgagcgc atccgcgagg
     4501 aaattcgcgg cggccgcacc gcgatttccg cgatcgatgc cggcttcaag cgggcattgt
     4561 cgacgattct ggattccaac atcaccacct tcatcgccgc ggcggtgctg ttctatatcg
     4621 gcaccggccc ggtgcgcggg ttcgccgtga cgctcggtat cggcatcatc accacggtct
     4681 tcaccgcctt cgagcttacc cgcctgatcg tcgcggcgtg ggtgatgtgg aagcggccga
     4741 gcagcgtgcc gatctaggca tgatcccgaa aagtggacac cggttttcgg aggagatcat
     4801 gccttcacag gataaagcga cgaatatgat ccagcctggc tggatcatat tcgggggaat
     4861 gcggagccag ctgtgacaca tttggtcctc atcgggctag gcatcctgat tgccgtgctg
     4921 acggtggtca gctgcttcgg cctgctgccg tcgctgcgaa tcgtgcctga cgataccaag
     4981 ttcgacttca cgcgttttcg ccgcatcagt tttccgattt cggcgctact ctcgattgtc
     5041 gcaatcaccc tgttctttac ccacgggctg aatttcggca tcgatttcaa gggtggcacg
     5101 ttgatggagg tgcgggccaa atccggcgct gccgaccttg catcgatgcg cagcacgctg
     5161 agtacgctgg gcgtcggcga cgtccaactg cagcagttcg gcggccccgc cgacgtgctg
     5221 atccgggtgg ccgagcagcc cggcggcgac gccgcgcagc aggcggcggt tcaaaaggtg
     5281 cgtggcgcgc tcggcgacgc cgtcgactac cgcagggtcg aagtggtcgg accgcgcgtc
     5341 tccggggagc ttttggccta cggcatgctc ggtctgatgc tcgcgatctt cgcgatcctg
     5401 atctatctct ggttccgctt cgaatggcag ttcgcgctcg gcgccatgat cgccaacgtc
     5461 cacgatatcg tgctgaccat cggcttcatg tcgatcacgc aggtcgattt cgatctcacc
     5521 agcatcgcgg cgctgttgac gattctcggc tattcgctca acgacaccgt cgtgatctat
     5581 gaccgcatcc gggaaatgct gcggcgctac aagaaaatgc cgatgccgga actgctcaac
     5641 gaatcgatca actcgacgct gtcgcgatcg atcatcaccc acgtcacggt aacgctggcg
     5701 cttttggcgc tgttgttgtt cggcggccac gccatccaca gcttcaccgc ggtcatgatg
     5761 ttcggcgtgg tgctggtcgg cacctacacc tcgatcttca tcgccgcgcc gatcctgatc
     5821 tatctcggcg tcggcaccaa ccgggccgac gcgccggaca cgccgaagaa gaagtagtat
     5881 tggactgtag ttatggcggt cccacctgac gccccgcatc ttccaaggtc ggcgccgatc
     5941 gaggcctacg gcaggtacgg atttgcgttc gccgacatgt cgcatcgcgg ctcattgctg
     6001 tgcctgccgg aggcgatctg ggcctggccg gtcacgaggc ctgaggatat cgacgaggct
     6061 tcgctggccc gcgtgttcgc cgcagccaat ggcatcgaca ccctgatcat cggcaccggc
     6121 accgacgtct ggctgccgcc gccggcgctt cgcgaggcct tgcgcgcggt gagggtggtg
     6181 ctcgacgcga tgcagaccgg ccccgcgatc cgcacctaca acatcatgat cggcgagcgg
     6241 cggcgggtcg cggcggcgct gatcgcggtg ccatgagcgg cgcggacggg cttgcggatt
     6301 ccgctgattt ctgcgcgcaa ttggtgcgaa ccaacgattt tgcccgctac gcatccacgc
     6361 tgttcgtgcc ggccggcgag cgccgggcgc tgttggcgat ctatgccttc aatgtcgaga
     6421 tttcccgggt ccgcgagcag gtgagccagc cgctgccggg cgaaatgcgg ctgcaatggt
     6481 ggaccgacat gctggcgggt gaaggccatg gcggtatcga gggcaatccg gtcgccgccg
     6541 agctgcagct tgcgatccag gattggcagc tgccggtcga gcggctgtcg cgcctgatcg
     6601 acgagcatca gttcgatctc tacaacgacc cgatgccgac catggcggcg ctggaaagct
     6661 acctcaacga taccgcgtcg gcgctgtttt cgctcggcgc aggcatcgcc ggggggcagt
     6721 ccggcgagat cgaacacctc gcgcgccacg ctggcctcgc gcaaggcatt gcccaggtga
     6781 tggcggcatt gccgcgggac gcttcgcggc gccagttgtt cgtgccgctg caggtgctgc
     6841 agcaacatgg cagcgggatg gaagaagtgt ttgcaggcaa gcaaacccca aagctgcgtg
     6901 cggtgctgga tcagctggtc ggcgatgcgc aggggcattt gaagaccgcc ttcgcgctgc
     6961 ttgcaaccgt gccgccgcag gtgaggccgg tgttcctgcc gctcaagctg gttgcccgtg
     7021 agctgatgcg aatgtcgcgg gcggatagcg atccgttcgc gccgtgggtg cgttcgagat
     7081 tgcggacgct atggacgttg tggcgggcgt cgcggtcgcg ggagtttaga gacggcggtt
     7141 gacgcaagcg ttccccggat gctgcgcagc gccttcagcg cgtttacgcg cgtcttcgac
     7201 gcgctatggc ggtgcgctgc tgaatccggg gtccattagc gccggcgact atatgggtcc
     7261 cggctctgcg gagcagcgtg aagtacgctg caccgcgtcc gggacacgat gcttcacgat
     7321 gacggcggcg ccagatcggc cgcctcgaaa ttgaaccggt cccgcagatt cttgcgcgat
     7381 tccagaatgc tcttcaggaa tgcgcggtcg gcctcgctgg tcatcatcgg ttcgatcagg
     7441 tcgagctggt tcatcgcgac aagctgcagc atctcggtgc gaacttttcc ggcggcatcg
     7501 cgcggcaggg cctgtaccac ctggatatgt tcgggcggtt ttggaccttt cgccgcgttg
     7561 agaccgttgc gcagttgcgc ttcgagcgcg gtctgatcgg cctcgacaaa ggcataaagc
     7621 ccgacaccgg tgcggcggtc ggcgaacgcg acaatcgcgg tatcgcggac accgggattc
     7681 tttcggatca gatcgatcag caccggcgcg tcgttgacca gccgccggcc gccgccctcg
     7741 cgatcggtaa agttgaacag tccccgggtg atggcgcggt agaccttctt gccggtcaga
     7801 agccagatgc tggctacaag cgatttgcgc gcgaggattt ttcgttcctt tggcgtcagc
     7861 gcttgcggcg cgtagctgcg cttgtgcttc aggagatggc ggagatcttc gtacgccgcg
     7921 atgcggaaca gccggccgcg cgtcttgaag caggccgcga gctggaagtc cgttacataa
     7981 gcgttcccat cgctgccgcg cagccagttc tgctccttgg cgagatcgtt gtggcagatg
     8041 ccggcgcgat gcagtttgcg cagcgcctgc ttggcggaac gaaaataggc gacgtcgccc
     8101 gtgggttttg ccagatgcag cgccacgccg ttgatgaatc cgcgcaccag cgcctgccgc
     8161 ccggcccaca acagttccgg cccgacatgc aggtcgcgcg ccagcgccag cgcgcggcgc
     8221 tcgcgtgcga acaggtagcg cgccgggatg taggaccacc acggcacggc atcgagccgg
     8281 cgcagcaccg catcgacttc ccccgcctcg ctgcgaaagc gaccgcgctc gacggtggaa
     8341 aacacgtcgc gcttcaacaa cacgccttcg atccatcgcg ccgacagaac cgcggcgtca
     8401 tccctgggca aactcatgaa acgctcacgc cgccgccgct acatggaggc tgtcggcaat
     8461 ccagcgatcg aggtccgaaa gcgcgcgggc gctcatcgcc tgcttcttcg caatggtctt
     8521 gtcgttgcca cgcagccgtg tgccttcggg cttttttgtc ggcggactgg cgagcggcgg
     8581 aaaaagtcca aagttgatat tcatcggctg gaacgagcgc gcaaccggat cgatggtctc
     8641 gatatggccg ccggtgatgt ggctgagcaa cgatcccagc gccgtcgtcg ccggcggcgg
     8701 cgccagcgac gtcgagcggg catcg
//
LOCUS       DASWGM010000464        10242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1988550,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000464 DASWGM010000000
VERSION     DASWGM010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988550"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VGA15_13385"
     CDS             complement(<1..1121)
                     /locus_tag="VGA15_13385"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX9588721.1"
                     /translation="MPTPKPPAFETLSLHAGQHPDPVTGARAVPIYQTTSYVFQDSEH
                     AAALFNLERAGHIYTRISNPTTAVLEERLAALEGGVGAICTASGMAALHLGIATLLGA
                     GGHIVASSSLYGGTINLLAHTLPRFGITTTFVKPRALDEFAAAIKPNTRLLIGETIGN
                     PGLEVLDIPNVARIAHDAKIPLLIDNTFATPYLSRPIELGADIVMNSATKWIGGHGIA
                     IGGVIVDGGRFDWRLSGKFPVLTEPYAGYHGIVFDEQFGPSAFIMRARTEGLRDFGAC
                     LSPTNAFQLLQGVETLGVRMDRHIENTHAVLEFLTANKAVDWVLHPSLENHPDYALAK
                     RLLPRGAGSIVSFGIKGGRPAGKKFIESLRLISHLANVG"
     gene            1316..2323
                     /gene="dctP"
                     /locus_tag="VGA15_13390"
     CDS             1316..2323
                     /gene="dctP"
                     /locus_tag="VGA15_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9588722.1"
                     /translation="MSGAKGNVIWRATAAMFALALLSAPVRPAVAAEKWDLYVYNPVS
                     TVAAVKGLNTVIEQIEKETGGELSIRLHLGGSLPINTTTITQAVSDDVVQMGDDGYFL
                     GNVPIGGVLRLPMLLRTLADYDKAAAIMNPYLEKAFEKKGLLVLGQYLYPFQVAFSSK
                     KLASLADIKGQKIRVTSPEQSEFIKRLGGIPVTIGAPQVPSALDRGVVDGVLTASTGG
                     GNIWKDLLKYNYRLGINYFNSVLIVNKERFAKLSPDVQGKVRRIVGDNMPLITKAMAD
                     DEDDLTRRFGDGGMTVTKEDPQDIEAGTKLIAPYWEEWARTQGPDAIAALKQVRTAIG
                     R"
     gene            2326..2874
                     /locus_tag="VGA15_13395"
     CDS             2326..2874
                     /locus_tag="VGA15_13395"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9588723.1"
                     /translation="MATQGEPVADGVAPHGIENVIPGSTLVEDACEIVCALFLVASIV
                     LIAAEAIARNVFATSFQITDEVCGYLLLALTFLSMSVAEAHGAFHRVELVQTRLSFAA
                     RLISQMVFDFISLVASALVTWQLYRLAANSWRAEDVAPTPLQTPLWLPQSAMAIGMLL
                     LCFALIRTMVTKARILRRGDRS"
     gene            2871..4178
                     /locus_tag="VGA15_13400"
     CDS             2871..4178
                     /locus_tag="VGA15_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9588724.1"
                     /translation="MTPGVELIVVMLAFLGLLTVGMTIPFAILVPSVLYLVMHAGLAG
                     LKGIGLVSWGSMNSFTLTAIPLFILMAEILQESKLSQRVYRGLSTLVAWMPGGLLQTN
                     IAGCAIFAAISGSSVVTAASIGRVALPELQRRNYSPRLAAGSLAAGGTLGILFPPSIA
                     MIVYGTFTETSVAKLFMAGVMPGLLLTFMFMSYIAVHAWLKPGVAPVERGERSLAELL
                     TAVLDVLPFVILIGGTMGSIYSGLVTPTEAAAVGCLLAMAIAMICGEFNFRVLKTALQ
                     TCVRVCGNILFIVYAAFVFSYAISYAGVGEQITQFLVDLKLSRLEFFLALFVLYTVLG
                     CLVESLGMIVVTVPLLYPVLLQYGIDPIWFGVILVLFIEMGQISPPIGINLFVIQSIW
                     TGKLSDVVMGTVPFHLLLFVLLGLLVVFPDIAMWLPSRTTVVP"
     gene            complement(4268..4522)
                     /locus_tag="VGA15_13405"
     CDS             complement(4268..4522)
                     /locus_tag="VGA15_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588725.1"
                     /translation="MIVMIILYLWAASAWAGLAVGFVFFRVLAIVLTSPVVALLSAIL
                     LHHYGFGLVRVVSISMGSLAALQSFYLLGAAMRYAAMNDE"
     gene            5106..5507
                     /locus_tag="VGA15_13410"
     CDS             5106..5507
                     /locus_tag="VGA15_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588726.1"
                     /translation="MARRLFQSEEGSARSLGPRRSETLAVEEQQEIVPRSSRLRAEST
                     SVWSDIMQKLPVELLAIAVLIELICLVGAERHANPVPVSIESTELWYNPWPAPGDQTL
                     VANGQCGIPSGVCVPADGSRSSASNGVRAAF"
     gene            complement(5609..6601)
                     /locus_tag="VGA15_13415"
     CDS             complement(5609..6601)
                     /locus_tag="VGA15_13415"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9588727.1"
                     /translation="MKHNSAICPKGTNGLLTVCFSTEEIPGADRVARWRDHHASVALN
                     VEIEPAQDHPFEACEVSRILPALHLMSLTMSAARMMRTRVTDGNDEFSLLVNRTGQLT
                     VSGRGREVPLAPGDGVLISSGDVMVCDRLSYGESLSLRVPRPILSSIVAGVDDAVMRP
                     ISGQSETLKLLTCYAATLIDQNALATPYLRNLAVHHVHDLIALALGRTRNVGGAANTE
                     GLRAARLHAAKVYLVENSSQHDLSVGVIAAHLAVTPRYLQRLFEADGTTFSAFLLAQR
                     LARAYRMLCDSQFSDRPVGAIAYSVGFGDLSYFNRCFRRQYGATPRDVREASAR"
     gene            complement(6949..7971)
                     /locus_tag="VGA15_13420"
     CDS             complement(6949..7971)
                     /locus_tag="VGA15_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9588728.1"
                     /translation="MTGTYRSLSRLLRMDRGREICPRATNDLATLRFTTEDIPEADRV
                     ERWRDHHLSLGQIEPAQDHPFEAREISCTLPMLHLMSLTLSASRIVRTRVIEGNDEVA
                     LFVNRTGRAMISARGREAPLAPGDGVLISCNDAMVYDRLSHGELLSIRVPRSVLSPVV
                     VGIDDAIMRPIPWHSETLKLLTYYAGTLIDGKALAASFLRNLAVNHLHDLIALTLGAT
                     RDATGAANAEGLRASRLRAAKAYIVENSSWHDLSVGAVAAQLGVTPRYLQRLLEADGT
                     TFSAFLLGQRLTRAYRMLCDLQFYSRPIGIIAYDVGFGDLSYFNRCFRRRYGVTPTDI
                     REACAK"
     gene            8712..9758
                     /locus_tag="VGA15_13425"
     CDS             8712..9758
                     /locus_tag="VGA15_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588729.1"
                     /translation="MTDRRRHLFTRTDCSFSRRRFLATSAAIAGGLGAASLIGSSGAR
                     AATLKTVRFSEAVHNLGYINLYVGMHAAIFKKNGLDMQVSAAGGDTQTFAAVLGGSAD
                     FAIGDATMAQISRENGGPGVVVGTVVQRAHYFGVSKTLQPITDPMGFKGLKIVTSPEP
                     NTNYSVAKRLLEKAGLKVGVDATIVPVNPGTEIAAMLAGQADIAIAYQPSVAAAEAQG
                     AKVVFDFSSYIGPFCNTGIMVLPTTIAKDPEMVQALVTSFEEASRKTYADPAFSKEVA
                     RKEFPDLPGDVVDKAIDAELKFLIPAQSVVTKQDQWKNLMDMQIYLGNAKGSIPFDQI
                     IDNTFADKAVASLK"
     gene            9755..>10242
                     /locus_tag="VGA15_13430"
     CDS             9755..>10242
                     /locus_tag="VGA15_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9588730.1"
                     /translation="MTRTGVSQSAGNAETVIGGVAIPPLVVEHVTKSYEVGGRIVPVI
                     GDLSLTLREGEIVSVVGPSGCGKTTLLNTLCGLLTPDSGRIRWHGREVSGQPQNVGYM
                     LQKDLLLPWRTALGNAMLGLEIRGVAPSEAEDRGHAMLDQLGLHGFAHHYPSTLSGGM
                     RQR"
BASE COUNT         2009 a         2995 c         3201 g         2037 t
ORIGIN      
        1 tcgccgacat tggcgaggtg gctgatcagc cgcaagctct cgatgaattt cttgccggca
       61 ggccggccgc ccttgattcc gaagctgacg atcgaccccg cgccgcgcgg cagcagtcgt
      121 ttggccagcg cataatccgg atggttttcc agcgaaggat gcagaaccca gtctaccgcc
      181 ttgttcgcgg tcaggaattc cagaaccgca tgggtgtttt cgatatggcg gtccatgcgg
      241 acgcccagcg tctcgacacc ctgcagcaat tgaaaggcat tggtcggcga gaggcaggcg
      301 ccgaagtcgc gcaggccctc ggtacgggcc cgcatgatga acgccgatgg gccgaattgc
      361 tcgtcgaaaa cgatgccgtg atagccggca tagggctcgg tcagtaccgg aaattttccg
      421 gaaagccgcc aatcgaacct gccgccatcg acaatcacgc cgccgatggc gatgccgtgg
      481 ccgccgatcc acttggtggc ggagttcatc acaatatcgg cgccaagttc gatcgggcgg
      541 ctgagatagg gcgttgcgaa ggtattgtcg atcagcaatg gaatttttgc atcgtgggcg
      601 atccgcgcga cgttggggat gtccagcact tccaggccgg gattgccgat ggtctcgccg
      661 atcagcaggc gcgtattggg tttgatcgcg gcggcgaatt cgtccagagc gcgcggcttg
      721 acgaaagtcg tggtgatgcc gaaacgcggc agcgtgtgcg ccagcagatt aatggtgccg
      781 ccgtaaagcg acgaggaggc cacgatatgg ccgccggcgc caagcagcgt ggcaatgccc
      841 agatgcagcg ccgccatgcc gctcgccgtg cagatggcgc cgacgccgcc ctcaagtgcg
      901 gcaaggcgct cctcgagcac cgcggtcgtc gggttcgaaa tccgggtata aatatgaccg
      961 gcacgctcga gattgaacag cgccgcggcg tgctcggaat cctgaaacac gtaggaggtg
     1021 gtctgataga tcgggacggc acgcgccccc gtcacgggat ccggatgctg tcccgcatgg
     1081 aggctgaggg tctcgaaagc gggcggcttg ggtgtgggca tcgcggctcg caaatgatga
     1141 gcagatgatc tgaacgcaca ggtccggaag atcgccggat gcccgcgtcg tatgccacaa
     1201 acccgagcgg aatgttagtg ccggcgcgca tgggagcaag tttttaccgg cgcttgcccg
     1261 ccctgcggcg atcgaggata ttgaaaaaaa cagaagacta aagggagcga aaacgatgtc
     1321 cggggccaaa ggcaacgtca tctggcgtgc gacggcggcg atgtttgcgt tggcgcttct
     1381 gtcggcgccg gtcaggccgg ctgtggcggc cgaaaaatgg gatctctacg tctacaatcc
     1441 cgtttcgacg gttgcggccg tcaaaggcct gaacacggtc atcgagcaga tcgaaaagga
     1501 aaccggcggc gagctgtcaa tcaggctgca tctcggaggt tcgctcccga tcaacaccac
     1561 caccatcacc caggccgtca gcgacgacgt cgtgcagatg ggagacgatg gatattttct
     1621 cggcaatgtg ccgatcggcg gcgtgctgcg gctgccgatg ctgctgcgga cgctggcgga
     1681 ttacgacaag gccgcggcca tcatgaaccc ctatctggaa aaggcattcg agaagaaggg
     1741 actgctggtt ctcgggcagt atctctaccc tttccaggtg gcgttttcga gcaagaagct
     1801 ggcgtcgctc gccgacatca agggtcagaa aatccgcgtg acatcgcccg aacagagcga
     1861 gttcatcaaa agactgggcg gcattcccgt gaccatcggc gcgccgcaag ttccctcggc
     1921 acttgatcgc ggcgtcgtcg acggcgtcct caccgccagc accggtggcg gcaacatctg
     1981 gaaggacctg ctcaaatata actatcgcct cggcatcaac tacttcaact cggtcctgat
     2041 cgtgaacaag gagcgcttcg caaagctctc gccggatgtt caaggcaagg tccgcaggat
     2101 cgtcggcgac aacatgcccc tgatcaccaa ggcgatggcg gatgacgagg acgatctgac
     2161 cagaagattc ggcgatggcg gcatgacggt gaccaaggaa gacccgcagg acatcgaggc
     2221 ggggacaaaa ctcatcgcgc cctactggga agaatgggcc aggacgcaag ggcccgacgc
     2281 gatcgccgcg ctcaagcagg ttcgcaccgc cattggccga taatcatggc aacacagggc
     2341 gagccggtgg ccgatggggt ggcgccccat ggcatcgaaa acgtcattcc cggatcgacg
     2401 ctggtcgagg atgcctgcga gatcgtctgc gcgctgttcc tggtggcgtc gattgtgctg
     2461 atcgcagccg aggcgatcgc gcgcaacgtg ttcgcgacgt cgtttcagat cacggatgag
     2521 gtctgcggct atctgctgct ggcgctgaca ttcttgagca tgtcggtcgc cgaggcgcac
     2581 ggcgcgttcc atcgcgtcga gctggttcag acccgtctgt ccttcgcggc gcggctgatc
     2641 tcgcaaatgg tgttcgattt catctcgctg gtggcgtccg cgctggtgac ctggcagctg
     2701 taccggcttg ccgccaattc gtggcgcgcc gaagacgttg cgccgacgcc gttgcagacg
     2761 ccgttgtggc tgccgcaaag cgcgatggcg atcgggatgc tgctgctgtg ctttgcgctg
     2821 atacggacga tggtgaccaa ggcgaggatt ttgcgcaggg gtgatcgatc atgacgccag
     2881 gcgtcgaatt gatcgtcgtg atgctggcct tcctcggcct gctcacggtg ggaatgacca
     2941 tcccgttcgc gattctggtc ccgtccgtgc tctatctcgt gatgcatgcc ggccttgccg
     3001 gactgaaagg catcgggctg gtcagctggg gcagcatgaa cagctttacc ctgacggcga
     3061 tcccgctgtt catcctgatg gccgagattc ttcaggagag caaactcagc cagcgcgtct
     3121 atcgcgggtt gtcgaccctg gtcgcgtgga tgcccggcgg gttgctgcag accaatatcg
     3181 ccggctgcgc cattttcgcc gcgatcagcg gatcgagcgt ggtgaccgcg gcatcgatcg
     3241 gccgcgttgc gctgccggaa ttgcagcggc gcaactacag tccgaggctg gcggccggtt
     3301 cgctggccgc cggcgggacg ctcgggattc tgtttccgcc gagcatcgcg atgatcgtct
     3361 acggcacgtt caccgaaacc tcggtggcga aactgttcat ggccggggtc atgccgggat
     3421 tgctgctgac cttcatgttc atgagctaca tcgccgtgca tgcctggctg aaacccggcg
     3481 tggcgccggt cgaacgcggc gagcgctcgc ttgcggaatt gctgaccgcc gttctcgatg
     3541 tgctgccgtt tgtgatcctg atcggcggca cgatgggaag catctattcg gggctggtca
     3601 cgccgacgga agcggcggcg gtcggatgcc tgctggccat ggcgatcgcc atgatctgcg
     3661 gtgaattcaa tttccgcgtc ctcaagacgg cgctgcagac ctgcgtccgg gtctgcggca
     3721 acatcctgtt tatcgtctat gccgcattcg tgttttccta cgccatctcc tatgccggcg
     3781 tcggcgagca gatcacccag tttctggtgg atctcaaact gtcgaggctc gaattctttc
     3841 tggcgttgtt tgtgctctac accgtgctcg gctgcctggt cgaaagtctc ggcatgatcg
     3901 tggtgacggt gccgctattg tatccggtgc tgctgcaata tggcatcgat ccgatctggt
     3961 tcggtgtcat tctcgtgctg ttcatcgaga tgggacagat ctcgccgccg atcggcatca
     4021 acttgttcgt cattcaaagc atctggaccg gcaagttgag cgacgttgtc atggggaccg
     4081 ttccgttcca tctcttgctg tttgtattgc tcggcttgct ggtcgtcttc cccgacattg
     4141 cgatgtggct gccgtcgcgg acgacggtgg ttccgtaacg gtagcgccgc ggccgggcag
     4201 tttcgcgact ggccgcctcg ggtattttcg ccgctgtcga gtggctgcgt tcgaaaccgg
     4261 atcggcatca ttcatcattc atggcagcgt acctcatggc tgcacccaac agataaaagc
     4321 tttggagggc agcaagagac cccatcgata tcgacacgac acggactaac ccgaaaccat
     4381 aatggtgaag aagaatcgcg gagaggagcg cgaccaccgg tgaggtgagg acgatcgcca
     4441 acacccggaa aaacacgaag ccgaccgcaa gtccggccca agcgctcgcg gcccataaat
     4501 acaaaataat catcacgatc aatacctgtc atgacggaag cacttcgcgt ccggccagcg
     4561 tcacatgcga gccgcgcgaa acttctgtct gtgtgttgtt ggtttgctta tgcccgatgg
     4621 gctgcgcggc gatatgacgg cgcccgcatc gggctgcgtc gcccgatcgc agtatgcggg
     4681 tgcatttggc gtggtccggg cggacacaaa gcaagataga gagaagaaag cgttagcaag
     4741 agaggcaatt gcattcgact tttgctgtca tcaacgacga cgcgtcgtca ttgcctggat
     4801 gtcgtatgtt tggatcaagt gctgacgaaa aatcccggcc gcggtgttct tcaagtcgtc
     4861 gctgatgctc cgggcgggcc ttcgcatttc gcaggaataa tttaaccatt gagcgcaaac
     4921 cctctggcct ccatcgggat gcatcttgcg gccatggcgg ccgatggatc ggatcttaag
     4981 tccgtgtcgt aagtctgcgt cagccgctga agaagaacct cgactgcggt tctctccaac
     5041 tcgttgtcgc accagtccaa gccgcgcatc gccgatgcat ctagggtctg cgcgcgtcga
     5101 ttcaggtggc gcggcggctc ttccaatcgg aggagggcag cgctcggtca ctcgggccgc
     5161 gacgcagcga aacgctggcc gtcgaagagc aacaagaaat cgtgccgcgc agttcacggc
     5221 tgcgggctga atcgaccagc gtctggagcg atatcatgca gaaattacca gtagagttgt
     5281 tggccattgc cgttctgatc gagctgatct gccttgtcgg cgcggagcgg catgccaatc
     5341 ctgtccccgt atcgattgag tcgacggagc tgtggtataa tccatggccg gctcccggcg
     5401 accaaacgct tgttgctaac ggacaatgcg gaattccgtc gggcgtttgc gttcccgcgg
     5461 acggcagccg atccagtgcc tccaacggcg tccgcgccgc gttctagcta gggccggcct
     5521 ccatcgttcg aacctgagat ggttgcgtgg acccgctgcg atcgatggcg cgcgggcggt
     5581 cctgccatcg cgcctcattc gccgatcgct atctcgcact ggcttccctg acatcgcggg
     5641 gcgtcgcgcc atactgtctg cggaaacatc gattgaaata ggagagatct ccgaagccga
     5701 cgctataggc aatggcaccg accgggcgat cggaaaattg cgagtcgcac agcatacgat
     5761 aggcgcgggc gagtcgttga gcgagcaaga aggcggaaaa tgtcgtgccg tcggcctcaa
     5821 acaacctttg aagatagcgt ggcgtgaccg ccagatgggc ggcgatgacg ccgaccgaaa
     5881 ggtcgtgctg ggaactgttt tcgacaaggt aaaccttcgc tgcgtgcaac cgcgcagccc
     5941 gcagcccttc cgtgttcgcc gctcccccga cgttgcgcgt tctgccgagc gccagcgcga
     6001 tcaaatcatg cacatgatgg acggctaggt ttcgaaggta aggcgtggca agagcgtttt
     6061 ggtcgatcag cgtcgcggca tagcacgtca gaagcttcag ggtctccgac tgcccggaga
     6121 tcgggcgcat gacggcatcg tcgacgccgg ccacgatcga agagaggatc gggcgcggca
     6181 cccgcagcga cagcgattcg ccgtaggata agcgatcgca caccatgaca tcaccgctgc
     6241 ttatcaatac gccatcgccg ggcgcaagcg gcacctcgcg accgcgtcct gacactgtga
     6301 gctggccggt ccggttgacg agcagcgaaa attcgtcgtt gccgtcggtg acacgcgtcc
     6361 gcatcatgcg cgcggccgac atcgtgagcg acatcaaatg caatgccggc aggatgcggg
     6421 agacctcgca ggcctcaaaa ggatgatctt gcgcaggctc gatttccacg ttgagtgcca
     6481 cgcttgcatg atggtcgcgc cacctcgcca cccgatccgc cccggggatt tcctcggtcg
     6541 aaaagcacac tgtcaagagg ccattcgtac ccttgggaca gatggcgctg ttgtgtttca
     6601 cgcgagaacg ccacgataac gatcgatatg ccacggtcat gcctgcagtc tcctgatcag
     6661 cccggaaacg acagttcgag cgccagacga cgctgcacgc gttgggcgtc cgccgatgga
     6721 agaattgccg tcaaggaacc cacgcggcag agcacgcttt ccgccacgga ggtcaaataa
     6781 tttgggggtc gaatgtgcgg tgatacctag tctggggcct ccgtctccag aatgatgcct
     6841 gagttcaccg ccatggtcgt atcaacggcg gatgtgccaa aacgggcacg attttcaggc
     6901 cggagttcgc cgatttgcgg ctcggtttcc aatcccggtt gtcgatcgct attttgcaca
     6961 tgcttccctg atatccgtgg gtgtcacgcc ataccgtctg cggaaacacc ggttgaaata
     7021 ggagagatcg ccaaagccga cgtcataagc gatgatgcct atcgggcggc tgtagaactg
     7081 caagtcgcac agcatccggt aggcgcgtgt gaggcgctga ccgagcaaga aagctgaaaa
     7141 tgtcgtgccg tcggcctcaa gcagcctttg aagatagcgt ggcgtgaccc ccagctgggc
     7201 cgcaacggcg ccgaccgaaa ggtcgtgcca ggagctgttt tcgacaatgt aggccttcgc
     7261 cgctcgcagc cgcgacgcac gcaatccttc cgcgttcgca gcccccgtgg cgtcgcgtgt
     7321 tgcgccgagc gtcagcgcga tcaaatcatg cagatgattg acggccaggt ttcggaggaa
     7381 ggacgcggca agcgccttgc cgtcgatcag tgtcccggca taatacgtca gaagcttcag
     7441 tgtctcggag tgccatggaa tcgggcgcat gatggcatcg tcgataccga cgacgaccgg
     7501 ggagaggacc gagcgcggca cgcgaatcga cagtaattcg ccatgggata agcgatcata
     7561 caccattgca tcattgcagc ttatcaatac gccatcgcca ggggcgagcg gcgcctcgcg
     7621 accgcgtgcg gatatcatgg cgcggccggt ccggttgacg aacagcgcga cctcatcgtt
     7681 gccctcgatg acacgcgtcc gcacgatgcg tgaggccgac agtgtgagcg acatcaaatg
     7741 caacatcggc agggtgcagg aaatctcgcg ggcctcaaaa gggtgatcct gtgcgggctc
     7801 aatttgtcca aggcttagat gatggtcccg ccatctctcg acccgatccg cctcggggat
     7861 gtcttcggtc gtgaacctca atgtcgcgag gtcattggtt gctctcgggc agatttctct
     7921 gccacgatcc atccgaagaa gccgcgacag cgatcgatat gttccggtca tgcctgcagt
     7981 ctcccaatca gtccgaagcg gatgttcgag cggcgagccc gtcgcgcaaa acgacaccgc
     8041 gcgttgggga tccgccgatt cacgcggccg agctccgcta ccggaataaa ttcgagggtc
     8101 aatgttgagg tattactcgc cctgcggcct ccgtcccgcg agcatccctg agttcaccac
     8161 cgtggtagta tcagcggcgg aaatacccag gactggttgg atttggtgat gttggcgata
     8221 ccggcgaaat gccggcgccg aacataccgc cccagttccg ggatcaagcc gctgcacacg
     8281 ccggattgat acgcgcaaac cctagatgcg gcggcgacgc gaaacttggt ctggtgcgac
     8341 gactacttgg taaaaaacgc tatcggactt ttttcaaagg ctgatgcaaa tttgcgacat
     8401 ggatcgaaat ttgtggcgtc cgcggcgaaa tgcacccatc gcgtgcagcc gtttctttgg
     8461 gcgacgataa tgtcaaaatc gacatcttgg catttgatat gccaccggat tgttcatcga
     8521 gacgattctt gcagcacgca aattgcaaat cgcgatcagc aacttcgctg tctttggcac
     8581 gtcggcaacg ccgtgaattg cattgccctg tattgagcaa ttgctgtcgc ttctttcaac
     8641 ttgccgcttg cggttggtgg actagtcttt gttcatccat tgccggttta agtcgcaagg
     8701 agttccctgt catgaccgat cgccgccgcc atctcttcac cagaaccgac tgttcattca
     8761 gccgccgtcg cttcctggcg acgtcagcgg cgatcgcggg tggtctcggc gctgcttcgc
     8821 tgatcggttc gagcggcgcg cgcgcggcaa cactgaagac cgtgcgtttc agcgaggcgg
     8881 tgcataacct cggctacatc aatctctatg tcggcatgca cgccgccatt ttcaaaaaga
     8941 acgggctcga catgcaggtg agcgccgccg gcggcgatac ccagacgttc gcggcggtgc
     9001 tgggcggatc ggccgacttc gccatcggcg atgcgacgat ggcgcagatc tcgcgggaga
     9061 atggcggtcc cggtgtcgtg gtgggaaccg tcgtgcaacg tgctcattac tttggcgtgt
     9121 cgaagacgct gcagccgatc accgatccca tgggtttcaa gggattgaag atcgtcacat
     9181 cgcccgagcc gaacaccaac tacagcgtcg cgaaacggct gctcgaaaaa gccggcctca
     9241 aagtcggtgt cgatgccacc atcgttccgg tcaatccggg taccgagatc gccgccatgc
     9301 tggccgggca ggcggatatc gccatcgcgt atcagccgag cgtcgccgcg gccgaagcgc
     9361 agggcgccaa ggtggtgttc gatttctcga gctacatcgg cccgttctgc aacaccggca
     9421 tcatggtgct tcccaccacg attgcgaaag acccggagat ggtgcaggcg ctggtgacct
     9481 cgttcgagga ggcatcgcgc aagacctatg ccgatccggc tttctccaag gaggtcgcgc
     9541 gcaaggagtt cccggatctg ccaggcgacg tcgttgacaa ggcgatcgat gcggagctta
     9601 aatttcttat tccggcgcaa tccgtggtga ccaagcagga tcaatggaag aacctgatgg
     9661 acatgcagat ttatctcggc aatgccaagg gcagcattcc cttcgaccag atcatcgaca
     9721 acaccttcgc cgacaaggcg gtcgccagtc tcaaatgacg agaaccggcg tcagtcaaag
     9781 cgctggcaat gccgagacag tcattggtgg tgttgcgatc ccgccgctgg tcgtggagca
     9841 cgtgacaaag agctatgagg tcggcgggcg catcgtgccg gtcattggcg acctcagcct
     9901 gacgcttcgg gaaggagaga tcgtcagcgt cgtcgggccc tccggctgtg gcaagacaac
     9961 gctgctcaat accttgtgcg gtttgctgac gccggattcc ggacgcatcc gttggcatgg
    10021 ccgcgaggtg tccggtcagc cgcagaatgt cggatacatg cttcagaagg atttgctgct
    10081 gccatggcgg acggcgctcg gcaacgccat gctggggctt gagatccgtg gcgtcgctcc
    10141 gagcgaagcc gaggatcgcg gtcacgcgat gctcgaccaa ctgggacttc atggctttgc
    10201 ccaccattac ccttcgacgc tctcgggtgg gatgcgtcag cg
//
LOCUS       DASWGM010000465         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1988680,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000465 DASWGM010000000
VERSION     DASWGM010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988680"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VGA15_13435"
     CDS             complement(<1..925)
                     /locus_tag="VGA15_13435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9588731.1"
                     /translation="MPKAPITNITFGTMTLGYRGYGARVHDAATADAMLGAVLEFGHD
                     QLDTCSVYGDGTCEQMLGDLRAAERFGIATRIHSGSPPGSAALNTRGHEPENLKRVFK
                     ESLARLKTDKVKIFYLVTPDSATPIESTLSAVQELHDEGLFEEFGVSNFSAWQVAEAS
                     EIAARHGWIRPSVYQGLYNAITRAVEPELFKCLGNYGIRFHAYNPLAGGAFSRNFADS
                     AAVAAGSRFDSSTAQGKLYRDRYMNDAYLDALAEIHRSCEGHGLDAISVALRWLIHHS
                     QLNAERNDGIIIGASSLEHLRHNLTAVTEGPL"
     gene            1045..1938
                     /locus_tag="VGA15_13440"
     CDS             1045..1938
                     /locus_tag="VGA15_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9588732.1"
                     /translation="MELEAVVAIARLGSFRAAAIDLDVSPTAIGNAVSSLENRLGVRL
                     FNRTTRNVALTSAGREYVEAIAPALSDIRNAARAVADHSRKPTGTLRFSCSLGGGRQL
                     LMPFVFEYLRRYDRMKVEIVTEGRLIDIIKDGFDAGVRLAEAVPQDMIAVPLGPDTRH
                     VVVGAPSYFANKSRPTAPADLMDHECIRARYSSGALYRWEFERHGEAMRIDVPGSLTL
                     DEPDMMAQASRAGLGLAYVGEWMVADDITSGRLVEVLSEWTPSYPGYCLYYPGRRHMA
                     ASLRAFVDLAREMVFRNRAAS"
BASE COUNT          419 a          609 c          671 g          431 t
ORIGIN      
        1 gcaacggacc ttcggtcacg gccgtgagat tgtgccggag atgctccaac gacgaggcgc
       61 cgatgataat gccatcattg cgctcggcgt ttagctggga gtgatgaatc agccagcgca
      121 gcgccacgga gatagcgtca agaccatggc cttcacacga tcggtgaatt tccgccaagg
      181 catcgagata ggcgtcgttc atatagcgat cgcgatacag tttgccttga gcagttgagc
      241 tgtcgaaacg cgagccggca gctaccgcag cgctatcggc aaaattcctg ctgaaagcac
      301 cgccggccag cggattgtaa gcatggaagc ggatgccata gttgccgagg catttgaaca
      361 gttccggttc aaccgcacgc gtgatcgcgt tgtacagtcc ttggtagacg ctcggccgta
      421 tccatccatg gcgcgcggct atttccgatg cctccgccac ttgccacgcg ctgaaattgc
      481 tgacgccgaa ctcctcaaac aggccttcgt catgaagttc ctggacggcc gacagcgtcg
      541 attcgatcgg cgtggcgctg tcaggcgtca ccaaatagaa gatcttgacc ttatcggtct
      601 tcaggcgcgc cagggactcc ttgaagactc gcttcaggtt ctcaggttcg tggccgcgag
      661 tgtttagcgc agcagatccg ggtggcgatc cggaatgaat ccgcgtggcg atgccgaacc
      721 gttcggcggc gcggagatca cccaacatct gttcgcaggt tccatcgcca tagacagagc
      781 acgtgtcgag ctggtcgtgg ccaaattcca agacggcgcc aagcattgcg tctgcggtcg
      841 cagcgtcatg aacccgggct ccgtatccgc ggtagccgag cgtcatggtg ccgaaggtga
      901 tgttggtgat aggggctttg ggcattccaa ccgccttgct tcattgagta tcgctgcacc
      961 gtaacggact ttgatcgtcg cgataattga gataaactgc gatgtcgtat ctagagaatc
     1021 gtgacaatgt acaaaagtgg gctgatggaa ctcgaggccg tggtcgccat cgccaggctc
     1081 ggaagcttcc gtgcggcggc catcgatctg gatgtgtcgc cgacagcgat tggcaatgcc
     1141 gtttccagtc tcgagaacag gctgggcgtt cgcctcttca accgcacgac gcgcaacgtc
     1201 gccctgacga gtgctggccg cgaatacgtc gaggcaatcg cgccggcgct gtcggacatc
     1261 cgtaacgccg cccgggccgt cgccgatcac agccgcaagc cgacaggcac gctacggttc
     1321 agttgctcgc tcggcggggg acgccagcta ctgatgccct tcgtcttcga gtatcttcgt
     1381 cgctatgacc ggatgaaggt cgaaatcgta accgaagggc ggctgatcga catcatcaag
     1441 gatgggtttg atgccggggt gcgcctggct gaagcggttc cgcaggacat gatcgcggtg
     1501 ccgcttggtc cagatacgcg acatgttgtg gtcggtgcgc caagctattt cgcaaacaag
     1561 tcccggccca ccgccccggc cgacctgatg gatcatgagt gcatccgcgc tcgctattca
     1621 agcggcgctc tctaccgctg ggagtttgaa cgtcacggcg aagcgatgag gatcgatgtg
     1681 ccggggtccc tcacactcga tgagcccgac atgatggcgc aggcgagcag ggccggtctc
     1741 ggccttgcct atgtcggcga gtggatggtg gctgatgaca tcacctcagg caggctggtt
     1801 gaagttctaa gcgagtggac accctcctat cccggatatt gcctctatta tccaggacgc
     1861 cgccacatgg ccgcgagctt gcgcgcattc gtcgatctgg ccagggagat ggtctttcga
     1921 aatcgcgcgg cgtcttgacg gacaccggac cggtggtccg attcgcggca agtgcgcagc
     1981 aaccgaccac gctgaacgag gtgattgaag cgacgacttc cgagtttgtg aaggtttgcg
     2041 gatgtcggcc ccatgaaggt ggtgcggcgt gcacggcggg gccggcgttc gcaaacctcc
     2101 cagggaggaa accgcgggag atgtgttgag
//
LOCUS       DASWGM010000466         9084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1715315,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000466 DASWGM010000000
VERSION     DASWGM010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715315"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VGA15_13445"
     CDS             <1..677
                     /locus_tag="VGA15_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX9588733.1"
                     /translation="PGTLPGAVDLGLVENPNVTAAMFGIDVNYLQVKVNEGALLPTVQ
                     LQASVQQSYEQQMTIFRSFGASAIAQVSVPVYQGGAEYSLIRQSKETLAQQRLNLDQT
                     RDATRANVVTAWGQLVAGKAQVASAQSQVTASEIALNGVREEAKAGQRTTLDVLNAQQ
                     ALVNARVALVTAQHDRVVASYAVLNAVGRLSPQVLNLATTVYDPSVHYQQVRDSWFGV
                     RTPDGR"
     gene            988..1752
                     /locus_tag="VGA15_13450"
     CDS             988..1752
                     /locus_tag="VGA15_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX9588734.1"
                     /translation="MTQPAKVQEPSMEEILASIRRIIADDEAKPAVEKPSSPAVAAKL
                     EKPAPPAPASKAPVMNNIPPSAIPAAQVAAAKAVPPVKPAPPPPAPPPAAAAVPNSQD
                     DIDAMLAGLDEATSEAEIRPASPEVEVFELTDDMAVPEPPQPSFKKVEPQDDLEFTEM
                     AAAKAMHRRPAFEPPPFESEAPAPQQILSRSTVSAVESAFNSLANTVLSNNARTLEDL
                     VKEMLRPMLKSWLDDNLPGLVERIVKAEIERVSRGR"
     gene            2105..4987
                     /locus_tag="VGA15_13455"
     CDS             2105..4987
                     /locus_tag="VGA15_13455"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX9588735.1"
                     /translation="MIEKTYQPADIETRMSRIWEESGAFRAGRPERRDAKPFTIVIPP
                     PNVTGSLHMGHALNNTLQDILCRFERMRGRDVLWQPGTDHAGIATQMVVERQLMERQL
                     PGRRDMGRAKFLERVWQWKAESGGVIVNQLKRLGASCDWSRERFTMDEGLSRAVIKVF
                     VQLHREGLIYKDKRLVNWDPALLTAISDLEVQQIEVKGSLWYLRYPLQGKTFDPEDPT
                     TFIVVATTRPETMLGDSAVAVHPDDERYADLVGQHVLLPLVGRKIPIVADEYSDPEKG
                     SGAVKVTPAHDFNDFEVGNRHGLPRISVLDQEGHLALVGNEDYLRGLPDESEYFAEEL
                     HGIDRFAARKLIVTRLEDFGFLEKVEPNIHMVPHGDRSGVVIEPYLTDQWYVDAKTVA
                     QPAIAAVRSGATAFVPKNWEKTYFEWMENIQPWCISRQLWWGHQIPAWYGPDGKVFVA
                     ETEEEAVGNALGYYAEQEVITPEQGREMALDPQKRAGFITRDEDVLDTWFSSALWPFS
                     TLGWPDDETDVKRYYPTDVLVTGFDIIFFWVARMMMMGLHFMKEAPFSTVYIHRLVRD
                     EKGAKMSKSKGNVIDPLGVIDDFGADALRFALARAAAQGHDIKLSPHHVETNRNFATK
                     LWNAGRFAEMNGCVLPPGFDPANAKETLNRWIVHETFRTGREVTEAIRTCRFNDAANA
                     IYRFVWNVYCDWYLELAKPVLLSEEGDAKAETRAMVAWARDEILKLLHPFMPFITEEL
                     WAVTAKRDGLLALAPWSIKAGALPLEQMSLIAKDDPQFPPELVALAAEANATSDAADF
                     VDHSAEAEIGWVVDLVTAIRSVRAEMNIPPATLTPLLLAGASAETMGRAQRWNDVVKR
                     MARLADISFADTAPQGAVQLLVRGEVAALPLKGVIDLSAEKARLDKELAKADADIKRV
                     DAKLSNEKFVANAPEEIVEEEKEKREAAVARKARIVEALDRLRNAA"
     gene            5000..5416
                     /locus_tag="VGA15_13460"
     CDS             5000..5416
                     /locus_tag="VGA15_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9588736.1"
                     /translation="MLDHVSITVSDIAAAERFYDAVMKALEIVKVGRRDDWLGHGERA
                     RAAYPERVYLTIREGPKPEDAFSRHWCFKAKWRTQVDAFWDAGIAAGGSDDGAPGLRN
                     YHASYYGAFLRDPDGNRIEAVCHHTVRGGHNAGWRA"
     gene            5397..6242
                     /locus_tag="VGA15_13465"
     CDS             5397..6242
                     /locus_tag="VGA15_13465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9588737.1"
                     /translation="MQAGEPSHTARGAAAYRAIHQTLEGGIIFSDPFASQILDDETRA
                     RLDEIAADLSLRPMRLLIAARSRFSEDTLAACVARGVRQIVVLGAGLDTFSLRNPHAG
                     QGVRVFEVDYPATQGWKRERLKQAGLAVPSSLAFAPVDFERQSLADGLAAAGFQADRS
                     AFFQWLGVVPYLTRQAISTTLDFIAGVPDSEVVFDYAEPFENQPTDRRAHAMAMAART
                     ASLGEPWLSLFDPAELSEMLCNKGFGVVEDLGLAEIADRFYGDLKQDIRIGPGPHVVR
                     AQKVR"
     gene            6283..7698
                     /locus_tag="VGA15_13470"
     CDS             6283..7698
                     /locus_tag="VGA15_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9588738.1"
                     /translation="MSFLVIAYLCGDLHPDVLETWTLGRRFEWGYSKHPPLTGWVARA
                     WTSVFPLTNWSFQLMALTNSAIALWAVDLISRRFVSGDKRIVVLLLLMLLPTYQFHAQ
                     RFNANSVLLAAWPIAIYCFLRSFETRQVIWAVAAGATAALAMLGKYYSVFLVGSFVFA
                     AICHPQRRAYFGSSAPWVSTIAGFAALGPHLHWLAMTGALPFAYALAGHTGKASGPAI
                     IEALLFVLGIAMILAIPAMTWVLMAGSRLDKFSQDFRAMNPGLLLLFLVSVGTIVFPA
                     ITSVALGADMPPIWALQGLFLFAILIVVVAPVHAFYRNIHPLHEGRNFYQLSAAELTR
                     QWHEQSDTALPIVGGDDGLAFAMAFYSPDHPVYEESLVFTNTERLPRQAIVKRGWAAL
                     CFGGDVDCIDGMERIAARASRFVRSEFVVQSTLLGQRGATQRFSAVIVPPFTEGTITP
                     SPASGVTEDFSAIRRTRPKPD"
     gene            complement(8463..9053)
                     /locus_tag="VGA15_13475"
     CDS             complement(8463..9053)
                     /locus_tag="VGA15_13475"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX9588739.1"
                     /translation="MSKTLDEQDLGRPLKRAFFARSVHEVAPDLIGATLLVDGVGGII
                     VEVEAYHHTDPAAHSFRGPTPRNLVMFGPPGFSYVYRSYGIHWCLNFVCEKQGSASAV
                     LIRALQPTHGIAAMRRRRGPHDERSLCSGPGKLCEALAITIKHSELPLDAPPIALHAR
                     TEKPDVVTGVRIGITKAVELPWRYGLKGSKFMSKPF"
BASE COUNT         1686 a         2755 c         2854 g         1789 t
ORIGIN      
        1 tgccggggac gctgcccggc gcggtcgatc tcggcctggt ggaaaatccg aacgtgaccg
       61 ccgcgatgtt cggcatcgat gtcaattatc tccaggtcaa ggtcaacgaa ggcgcgctgc
      121 tgcccacggt gcagttgcag gcttcggtac agcaatccta cgaacagcag atgacgatct
      181 tccgctcgtt cggcgcctcg gcgatcgcgc aggtttcggt tccggtgtat cagggcggcg
      241 ccgaatattc gctgatccgc caatcgaagg aaacattggc gcagcagcgg ctcaacctcg
      301 atcagactcg cgatgcgacc cgcgccaatg ttgtgacggc gtgggggcag ttggtggcgg
      361 gcaaggcaca ggtcgcttcg gcgcaatcgc aggtcaccgc ttccgagatc gcgctcaacg
      421 gcgtgcgcga agaggccaag gccggacagc gaaccacgct cgacgtgctg aacgcccagc
      481 aggcgctggt caacgcccgt gtcgcgctcg tcaccgcgca gcatgatcgc gtggtggcgt
      541 cctatgcggt cttgaatgcg gtgggacggc tgtcgccgca ggtgctcaat ctggccacca
      601 ccgtgtacga tccgagcgtg cattatcagc aggttcgcga cagctggttt ggcgttcgca
      661 cccccgacgg ccgctgatcg cggactcgct gctacaggcg ttggaagacg ggccgcgtcc
      721 atagcgcgtt gcttgcaatc aattgttgcc atgacatacc tttattgggg gcaacagagc
      781 gattcgcgac gcatatgtat ccttaggacg cgacgcttgg gtgcctgagc aggggcggga
      841 accccgcgcg acgtttcgcg cgaggcttga tctggggaca agtcagggct tgtgcgatga
      901 aaatcccggc ctgcatttcc ggaaacggta atccttcttt gctaggtata aaacgcacac
      961 gagggcgttg atgatgtgga gtcggagatg acgcagcctg caaaggtcca agagccctcg
     1021 atggaggaga ttctggcgtc gatccggcgt atcatcgccg acgatgaggc aaaaccggcc
     1081 gtcgagaagc cctcaagccc ggccgtagca gcgaagctgg aaaaacccgc cccgccggcg
     1141 cccgcatcca aggcgcccgt gatgaataat attcctccgt cggcgatccc cgccgcgcag
     1201 gtggccgctg cgaaggcggt gccgcccgtc aaacccgcgc cacccccgcc cgcaccacct
     1261 cctgccgcgg ccgccgtgcc caacagtcag gacgatatcg atgcgatgct ggccggcctc
     1321 gatgaggcga cgtcagaagc cgagataagg ccggcctcgc ccgaggtcga ggtgttcgaa
     1381 ctcaccgacg acatggcggt gccggagccg ccgcagcctt cgttcaagaa ggttgaaccg
     1441 caggacgatc tcgaattcac cgaaatggcc gccgccaagg cgatgcatcg gcggcccgcg
     1501 ttcgaaccgc cgccgttcga aagtgaggcg ccggcgccgc aacagatatt gtcacgatct
     1561 accgtgtccg ccgtcgaatc cgccttcaat agcctcgcca acaccgtgct gagcaacaac
     1621 gcgcggacgc tggaagatct cgtcaaggag atgctgcggc cgatgctgaa atcctggctc
     1681 gatgacaatc tgcccgggct ggtggagcgc atcgtcaagg cggaaatcga acgggtttcg
     1741 cgcggacgtt agagcgtttt caagcgaaag cctgccccgg acttgatccg gggtggagac
     1801 cggttcgcat taagaaaacg cgtcaaatca agaatctaga gccccgtttc gtttctatcg
     1861 aaacggggct ctgcccattt tgcgcgtatt ctgatcgcaa aaccggtatc caccgtcgga
     1921 tcaagtccgg gtgctgttgc ggaaccggcg ttggtccgcg cgcggcgggt atttgttgag
     1981 cccgttcagc cactagatcc ggcctaatcc tgcctttcga tgggcctgtc gtgttgactt
     2041 ggtctgttga cttggcgcgt ttcggcgggt ttctagcccg ttccatagcc atgattgcat
     2101 tgccatgatc gagaagacct accagcccgc cgatatcgaa acccgcatgt cccggatctg
     2161 ggaggaaagc ggcgcgttca gggccggccg ccccgagcgc cgcgatgcaa aacccttcac
     2221 catcgtgatc ccgccgccga acgtgaccgg ctcgctgcac atggggcatg cgctcaacaa
     2281 caccctgcag gacatcctct gccgtttcga gcggatgcgc ggccgcgacg tgctgtggca
     2341 gcccggcacc gatcatgctg gcatcgcgac ccagatggtg gtcgagcggc aattgatgga
     2401 gcgccagctg ccgggccgcc gcgacatggg ccgcgcaaaa tttctcgagc gcgtctggca
     2461 atggaaggcc gaaagcggcg gcgtcatcgt caaccagtta aagcggctcg gcgcgtcctg
     2521 cgactggtcg cgcgaacgct ttaccatgga cgagggcctg tcgcgcgccg tcatcaaagt
     2581 gttcgtccag ctgcatcgcg aagggctgat ctacaaggac aagcggctgg tgaactggga
     2641 tccggcgctg ctgaccgcga tttccgatct cgaagtgcag cagatcgagg tcaagggcag
     2701 cctctggtac ttgcgctatc cgctgcaagg caaaaccttc gatcccgagg atcccaccac
     2761 ctttatcgtt gtcgctacca cgcggccgga gacgatgctc ggcgacagcg cggtcgcggt
     2821 gcatccggac gatgagcgct acgcggatct ggtcggccag cacgtgctcc tgccgctggt
     2881 ggggcggaag attccgattg tcgccgacga atattccgac cccgaaaaag gttcgggcgc
     2941 ggtgaaggtc acgccggcgc acgacttcaa cgatttcgag gtcggcaacc ggcacggcct
     3001 gccgcggatc agcgtgctcg atcaggaagg acatctcgct ctggtcggca acgaagatta
     3061 tctgcgcgga ttgccggatg aatcggagta ttttgccgag gaattgcacg gcatcgaccg
     3121 gtttgccgcg cgcaagctga tcgtgacacg gctggaagat ttcggttttc tggaaaaagt
     3181 cgagccgaac atccacatgg tgccgcatgg cgatcgctcc ggcgtcgtca tcgagccgta
     3241 cctgaccgac cagtggtatg tcgacgccaa gaccgtggcg cagcccgcca ttgccgcggt
     3301 gcgcagcggc gcgaccgcgt tcgtgccgaa gaattgggaa aaaacctatt tcgagtggat
     3361 ggagaacatc cagccctggt gcatctcgcg ccagctctgg tggggtcacc agatcccggc
     3421 gtggtacggc cccgacggca aggtgttcgt cgccgaaacc gaggaagagg cggtcggcaa
     3481 cgcgctcggc tattacgccg agcaggaagt cattacgccg gagcaggggc gggagatggc
     3541 gctcgatccc caaaagcgcg caggttttat cacgcgcgac gaagacgtgc tcgacacctg
     3601 gttctcctcg gcactgtggc cgttctcgac gctgggctgg cccgacgacg agacggacgt
     3661 caagcgctat tatccgaccg acgtgctcgt caccggcttc gacatcatct tcttctgggt
     3721 cgcccggatg atgatgatgg gtcttcactt catgaaagag gcaccgttct cgacggtcta
     3781 tatccaccgc ctggttcgcg acgagaaggg cgcgaagatg tcgaagtcga aaggcaacgt
     3841 catcgatccg cttggggtca tcgacgactt cggcgctgac gccctccgct ttgcgctcgc
     3901 gcgcgcggcc gcgcagggac acgacatcaa gctgtcgccc catcacgtcg aaaccaaccg
     3961 caattttgca accaagctgt ggaacgccgg ccgtttcgcc gagatgaatg gctgcgtgct
     4021 gccgccgggg ttcgatccgg ccaatgcgaa agagacgctc aaccgttgga tcgtgcatga
     4081 gaccttccgt acggggcgcg aggtgaccga ggcgatcagg acttgtcgct tcaatgacgc
     4141 cgccaacgcg atctatcgtt tcgtctggaa cgtctattgc gactggtatc tcgagctcgc
     4201 gaaacccgta ttgctgagcg aggaggggga cgccaaggcc gagacccgcg cgatggtggc
     4261 gtgggcgcgc gatgaaatcc taaaactgct gcacccgttc atgcccttca tcaccgagga
     4321 gctgtgggcg gtcaccgcaa agcgcgatgg gctattggcg ttggcgccct ggtcgatcaa
     4381 ggcgggtgcg ctcccgctgg aacaaatgtc gctgatcgcg aaagacgatc cgcagtttcc
     4441 accggaactt gtggctttgg ctgccgaagc gaacgcaaca agcgacgccg ctgatttcgt
     4501 tgaccactcg gccgaagccg agatcggctg ggtggtcgat ctcgtcaccg cgattcgctc
     4561 ggtgcgcgcg gaaatgaata ttccgccggc aacgttgacg ccgctgctgc tggccggcgc
     4621 gtccgctgaa accatgggac gcgcgcaacg ctggaacgat gtggtcaagc ggatggcccg
     4681 gctcgccgat atttccttcg ccgataccgc gccgcaaggg gctgtgcagt tgctggtgcg
     4741 cggcgaggtc gcggcattgc cgctcaaggg cgtcatcgac ctttcggcgg agaaggcgcg
     4801 gctcgacaag gaattggcca aggccgacgc cgacatcaag cgcgtcgatg ccaagctctc
     4861 caacgagaaa ttcgtcgcca acgcaccgga agaaatcgtc gaagaggaaa aagaaaagcg
     4921 cgaggcggcc gtggcgcgca aagccaggat tgtcgaggcg ctggatcggc tcagaaacgc
     4981 tgcgtaacga gaatggccga tgctggacca cgtttccatc accgtatcgg atatcgccgc
     5041 ggccgagcgc ttctacgacg cggtcatgaa agcgcttgag atcgtcaagg tcggccgccg
     5101 cgatgactgg ctgggccatg gcgaacgcgc gcgcgccgct tatcccgagc gcgtctacct
     5161 caccatccgc gaagggccaa agcccgaaga cgcctttagc cgccactggt gcttcaaggc
     5221 gaagtggcgg acccaggtcg atgccttctg ggatgccggc atcgccgccg gcggcagcga
     5281 tgacggtgca ccgggcctgc gcaattatca cgcatcctat tacggagcgt ttctgcgcga
     5341 ccccgacggc aaccggatcg aagccgtctg ccatcacacg gtcagaggag gtcacaatgc
     5401 aggctggaga gcctagtcac accgcgcgcg gagcggcagc ctatcgagcc atccatcaaa
     5461 ccctggaagg cggcattatc ttcagcgatc cgttcgcgtc ccaaattctc gacgacgaaa
     5521 cccgcgccag acttgacgaa atcgctgccg acctgtcgtt gcggccaatg cgcctgttaa
     5581 tcgcggcgcg cagccggttt tccgaggaca cgttggcggc ctgcgtggca cgcggcgtgc
     5641 gccagattgt cgtgctggga gcgggcctcg atacgttttc gctgcgcaac ccgcatgccg
     5701 ggcagggtgt ccgtgtgttc gaggtcgatt atccggcaac gcagggctgg aagcgcgagc
     5761 gcctgaagca ggccggtctc gccgtgccct cgtcgctcgc cttcgctccc gtcgacttcg
     5821 agcggcagag cctggccgat ggtctggcgg ccgccgggtt tcaggctgat cgctctgcgt
     5881 tcttccagtg gctcggtgtc gtgccgtatc tcacacgcca ggcgatctcc acgacgctgg
     5941 atttcatcgc cggtgtgccg gattcggagg tcgtgttcga ctacgccgag ccgtttgaaa
     6001 accagccaac cgatcgccgc gctcatgcga tggccatggc cgcaaggacg gcttcgctgg
     6061 gtgagccatg gctcagtctt ttcgatccgg ccgagttgtc cgagatgctg tgtaacaagg
     6121 gatttggggt cgtcgaggat ctcggccttg ccgaaatcgc cgatcgattc tacggagact
     6181 tgaaacagga catccggatt ggtccaggtc cgcacgtcgt tcgggcgcag aaggtccgtt
     6241 gatccatgtc cgcttctgct ggccggcttt gtagcggttt ggatgagctt tctggtcatc
     6301 gcctacctct gcggcgattt gcaccctgat gttttggaaa cgtggacgct cggacgccgg
     6361 tttgaatggg gttactccaa acatcctccg ctgacgggat gggttgcgcg ggcatggacg
     6421 tcggtgtttc cactcaccaa ctggtcgttt cagttgatgg cgttgaccaa ttcggcaatc
     6481 gcgttgtggg ctgtcgatct gatttcgcgc cgcttcgtaa gcggtgacaa aagaatagtc
     6541 gtgctgcttc ttctcatgct gctgccgact taccagtttc acgcccaacg cttcaatgcc
     6601 aacagcgttc ttttggcggc ctggccgatc gctatttatt gcttcctgcg gtcgttcgaa
     6661 actcgtcagg tcatatgggc cgttgccgcg ggcgcaaccg ctgcgcttgc gatgctgggc
     6721 aaatactatt cggtgtttct tgtcggcagc ttcgttttcg ctgcgatttg tcatccacaa
     6781 cgtcgcgctt atttcggttc atcagcccct tgggtttcaa ccatcgcagg ctttgccgca
     6841 ctcggaccgc atttgcattg gctcgcgatg accggcgcgc tgcccttcgc ttatgcgctg
     6901 gcgggacata caggcaaggc ctccggccct gcgataatcg aagcgctgct tttcgtgctc
     6961 ggtatcgcca tgatccttgc cataccggcg atgacatggg ttctgatggc gggaagccgc
     7021 ctcgacaagt tttcccagga cttccgggcg atgaaccctg ggctgttgct gctctttctt
     7081 gtcagcgtcg ggacaattgt ctttccggcc atcacttcgg tcgcgctggg agccgacatg
     7141 ccgccgatct gggcattaca aggtcttttc ctgttcgcca ttctgatcgt cgtggtggcg
     7201 cccgttcacg ccttctatcg gaacatccat ccattgcacg aggggcgtaa tttctatcag
     7261 ctgtcggcgg cggagttgac ccggcagtgg cacgagcagt ccgataccgc gcttcccatc
     7321 gtcggcggcg acgacggcct ggccttcgcc atggctttct acagcccgga tcatcccgtc
     7381 tacgaagaga gccttgtctt cacaaatacc gagcgattgc cgcgtcaggc gatcgtcaag
     7441 cgcggatggg cagccttgtg ctttggcgga gacgtagatt gtatcgatgg gatggaaagg
     7501 atcgcggcac gcgcttcccg gtttgtcaga tccgagttcg tcgtccagtc aacactgtta
     7561 ggtcagcgcg gcgccacgca gcgcttttcg gccgttatcg ttccgccatt cactgagggg
     7621 acgatcacac catcgccggc cagcggcgtc acagaagatt tcagcgccat tcgccgcacg
     7681 cggccgaaac cggactagct tctctgacga aatgtgctgc gccgcgcccg ggacacgcgc
     7741 ggaatttgct ctcgctgccg tgcggtgatt tgcccgtcgg gcggtttgtt gacaggggtg
     7801 tctagccccc tttgcaaaaa tatttctgtt ttcgcacccc caaatcacgt ctagaacttt
     7861 gcgcatcccg tcccacacag aggggcgttt cgcgatcgtc acggacgtag gggcgggatg
     7921 cagtggacgc ggcagcgttt tgcgcgcgat gggatcgcag ggcgagtcga aagacttgtg
     7981 agcgatcaaa gagcgcgctg acgagagatg ttgctgcgga cggcaaaatc gtgtggtcct
     8041 gacgccccga cgctggcgtc aagttttgtg gaaggcaagt cggccctacc gggtgcggac
     8101 aagccagata tccgcgagat gacggtggca aaagagcccg gtcaccgggg agagcacgac
     8161 ataagccgtt aaaaccattg cgtgcgggaa tgccgggtgt tcggcgtact cgtggtgact
     8221 aacgcgtgtg ctttctacac tgcacacgcg gctgcgggtg cgcggcaccc ggcattcccc
     8281 acgccctccc tggggcgaag ataaacgtaa cgactcgggc gcatcgcgcc gcggggtcgc
     8341 ggactcgtgt ttggatttgc cgtcattgcg aggagcgtag cgacgaagca atccattctc
     8401 gctttcttct ttgctgttga ttgcttcgct tcgctcgcaa tgacggtctc aagctctgct
     8461 ggtcaaaacg gcttgctcat gaatttcgag cctttcaatc catatcgcca cggcaattca
     8521 accgcctttg taatgccgat ccgcacgccg gtgacgacgt ccggcttttc cgtccgcgca
     8581 tgcagcgcga tcggcggggc gtcgagcggc aactcgctgt gcttgatggt gatggcgagt
     8641 gcttcgcaga gctttccggg tccagagcac agcgatcgct cgtcatgcgg gccgcgccgg
     8701 cgccgcatcg ctgcaatgcc gtgggtcggc tgcagggcgc ggatcaacac cgcgctggcg
     8761 gaaccttgct tctcgcagac gaaattcagg caccaatgaa tgccatagga gcgatagaca
     8821 taggaaaatc cgggcggccc gaacatcacc agattacgcg gcgtcggtcc gcggaacgaa
     8881 tgcgcggccg gatcggtgtg gtgataggcc tcgacctcga cgatgatgcc gccgacaccg
     8941 tcaaccagca gcgtcgcgcc gatcaggtcg ggcgccacct cgtgcacgct gcgggcaaag
     9001 aacgcacgct tgagcgggcg gcccagatcc tgctcatcca acgttttgct cattcaatgt
     9061 gcgattttga gccattccag gcgg
//
LOCUS       DASWGM010000467         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_881694,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000467 DASWGM010000000
VERSION     DASWGM010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881694"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGA15_13480"
     CDS             <1..334
                     /locus_tag="VGA15_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588740.1"
                     /translation="PAGPDWQKSIAKDVTRALIGVEMESPEPRALAEHWGRIIGIPVT
                     QNQGEEPELKLPNCSFRFVQGMSDIMSGLTFRVGDIAAVRDAAKTRGHPLSGDSFLLG
                     GVTFRLVA"
     gene            564..2687
                     /locus_tag="VGA15_13485"
     CDS             564..2687
                     /locus_tag="VGA15_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9588741.1"
                     /translation="MPHPLDSFFAPDSIALIGASRDQEKIPGRLLSMLRKNAFPGKLY
                     PINPNYGDIDGLKCFSSIADVGQPIDLAIVIIPARAVLGALEQCAAVGVKNAVIISSG
                     FAEEGGDSAAMQDAIVALAKKTGMRISGPNAEGFYSQVQRVAATFSPTVDVKPGAPRL
                     VATIRRIGIVAQSGGIGFAIYHRAKALGIALSYVISAGNESDLGAGEFLEYMVQDALT
                     DVILLFIEGIRDVDKFLAAAQRAAETGKPVIVTKVGRSGAGERAAASHTASMAGWSAA
                     YDAVFAKYGFIVSNDLDEAVTIAAVLTTNPLPKGDRVAVLTVSGGAGIWGADTVSMQG
                     LQVPELSKSIQAEISALLPSYGATGNPIDVTAQGVHSGGLQRSIDLLDVSDEVDAILV
                     VLSLSSDTRMPFKQAELKPVVSAQNKPIVFYSYTLPSDFARRELAASGAAVLSGLTHV
                     GVAMRQMVARARFRLAPAADAAALPLRDISAHLKSATLSEFDSKALLRDAGIALPDEV
                     LVSKRDELDAALARVGFPLVIKIQSRDIPHKSEVGGVRVNIATKGEAFAAYQALLESA
                     RRHRPDAVIQGVLVSPMAKKGVEIIVGTLLDATFGPMIMVGLGGITTELFRDVIYRPA
                     PVSAVEASAMLAELKAAPLLNGFRGAAKTDIPALSHLISQISVLAARFCAEISEIEIN
                     PVLVHPQGQGVTIVDALVVPRKKKA"
     gene            complement(2863..>3018)
                     /locus_tag="VGA15_13490"
     CDS             complement(2863..>3018)
                     /locus_tag="VGA15_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9588742.1"
                     /translation="TRATMRAGLADRVMAATNHELAEQTRLRATEDFKEGIKATEERR
                     VANFRGR"
BASE COUNT          531 a          939 c          974 g          574 t
ORIGIN      
        1 gccggcaggt ccggactggc agaaatcgat cgctaaggat gtgacgcggg cgctcatcgg
       61 cgtggagatg gaaagtccgg agccgcgggc gttggccgaa cactggggca ggatcatcgg
      121 cattcccgtc acgcaaaatc agggcgaaga gcccgagctg aaactcccca attgcagctt
      181 ccgcttcgtt cagggtatga gcgacatcat gagtggcctg acgtttcggg tcggcgatat
      241 cgccgccgtg cgcgatgccg cgaaaacaag gggccatccg ctgtcgggcg attctttcct
      301 gctcggcggc gttacgtttc gtctcgtggc gtaagcgaaa agcgcggatg gagccaacgg
      361 gcggcagtcg tcgttccccg gatgctgcgc agcgccatca gcgcgtttac gcgcgtcttc
      421 gacgcgctat gacttgcggc gtggtgcgct gctgatccgg ggtccacact cgcctctgtg
      481 ggtcccggct ctgcggagca gcgttacacg ctgcaccgcg tccgggacac ggcactcgaa
      541 cttaaagtgt aggatcgttc cgcatgccgc atccgctcga ttcgttcttc gcgcccgaca
      601 gcatcgccct gatcggcgcc tcgcgcgacc aggagaaaat cccgggccgg ctattgtcga
      661 tgctgcgcaa gaacgcgttt cccggaaagc tctatccgat caatcccaat tacggcgata
      721 tcgacggcct aaaatgcttt tcctcgatcg ctgatgtcgg gcagccgatc gaccttgcca
      781 tcgtcatcat tccggcgcgc gcggtgctcg gcgcgctcga gcaatgcgcc gccgtcggcg
      841 tcaagaacgc ggtgatcata tcgtcgggtt tcgccgagga gggcggcgac agcgccgcca
      901 tgcaggacgc cattgtcgcg ctggccaaga agaccgggat gcggatttcc ggccccaatg
      961 ccgaaggatt ctacagccag gtccagcgcg tcgccgccac cttcagcccg accgtcgatg
     1021 tcaaaccggg cgcgccgcgg ctggtcgcga ccatcaggcg gatcggcatc gtcgcgcaaa
     1081 gcggcggcat cggttttgcg atctatcacc gcgccaaggc gctggggatc gcgctgagct
     1141 acgtcatcag cgccggcaac gaatccgacc tcggcgccgg cgaatttctt gaatacatgg
     1201 tgcaggatgc tttgaccgac gtgatcctct tgttcatcga gggcattcgc gacgtcgata
     1261 aatttctggc cgccgcccaa cgggctgcgg aaaccggcaa acccgtcatc gtgacaaaag
     1321 tcggccgctc cggcgctggc gagcgcgccg cggcatcgca taccgccagc atggcgggct
     1381 ggtcggcggc ctacgacgcg gtatttgcaa agtacggttt tatcgtctcc aacgatctcg
     1441 atgaggctgt taccatcgcc gcggtgctga ccaccaaccc gctgccgaaa ggcgaccgcg
     1501 tggcggtgct gacggtgtcg ggcggcgccg gtatctgggg cgcagacacg gtttccatgc
     1561 agggcctgca ggtgccggaa ctttccaagt cgatccaggc ggaaatcagc gcactacttc
     1621 cgtcctatgg ggcaacaggc aacccgatcg atgtcaccgc gcagggcgtg cattctggtg
     1681 gcctgcagcg gagcatcgat ctgctcgacg tctcagacga agtcgatgcc attctggtgg
     1741 tgctgtcgct atcgagcgac acgcggatgc cgttcaagca ggcggaactg aagcctgtcg
     1801 tatcggcgca gaacaagccg atcgtgttct actcctacac gctgccgtcc gatttcgccc
     1861 gaagggagct tgcggcgtcg ggcgccgcgg tgctgtcggg attgacccat gtcggggtcg
     1921 ccatgcgcca gatggttgcg cgcgccagat tcaggctggc gccggcggcc gatgccgcgg
     1981 cgttgcctct gcgcgatatt tccgcacatt tgaaatccgc gacgctttct gaattcgaca
     2041 gcaaggcgct gctgcgcgat gccggcattg cgttgccgga cgaagtgttg gtgtccaaga
     2101 gagacgaact ggacgcagca cttgcccgcg tcggctttcc gctcgtgata aaaatccagt
     2161 cgcgcgatat cccgcacaag agcgaagtcg gcggcgtgcg cgtcaacatc gcgaccaagg
     2221 gcgaagcctt cgcggcctac caggcgctgc tggaaagcgc gcgccggcat cggcccgatg
     2281 ccgtcattca aggcgtgctc gtcagcccga tggcaaaaaa gggcgtcgag atcatcgtcg
     2341 gcaccctgct cgatgccacc ttcggtccga tgatcatggt cggcctcggc ggcatcacca
     2401 cggaattatt tcgggacgtg atctatcgcc ccgcaccggt gagtgccgtc gaggcatcag
     2461 ccatgctggc cgagctgaag gcagcaccct tgctcaatgg ctttcggggg gcggcgaaaa
     2521 ccgacatccc ggcattgtcg catttgattt cgcagatatc cgtccttgca gcgcgatttt
     2581 gtgcggagat ttccgaaatc gagatcaatc cggtgctggt gcatccgcaa gggcagggcg
     2641 tcaccatcgt cgatgcgctg gtggtgccgc ggaagaagaa ggcctagcct tctccgcgag
     2701 tcgtcccggc gaacgccggg acccataacc cctggcgttg atcgttaaag aaggcgtctg
     2761 ccacagtgcc gaaaacgtga atccgcgccg ccgcgatgag cgcaattgcg ctcacgctgg
     2821 ggtcccggcc ttcgccggga cgacgtgttg aggcggatgc gctcaccgcc ccctgaaatt
     2881 cgcgacgcgg cgttcttcgg tggccttgat gccttccttg aagtcttcgg tggcgcgcag
     2941 ccttgtttgt tcggcgagtt cgtgattggt cgcggccatc acgcggtcgg cgaggccggc
     3001 gcgcatggtg gcgcgggt
//
LOCUS       DASWGM010000468         8663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2666599,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000468 DASWGM010000000
VERSION     DASWGM010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666599"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VGA15_13495"
     CDS             complement(<1..909)
                     /locus_tag="VGA15_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEX9588743.1"
                     /translation="MPFDLVLRGGRVIDPSQKLDAVTDVAFAAGKVVRVGNELSVDPG
                     TDVRDVSGYIVTPGLIDLHTHVYWGGTSIGIDAEEFCRTSGVTTAVDSGSAGPGNFAG
                     FRKHVIEPSQVRILAYLHVSHAGIFGFSHRIMVGESEEIRLMNPIDAVRVAELNRDLI
                     VGIKVRVGQHSSGTSGIVPLEIALEVAEEVGMPLMAHIDHPPPSYEEVLARLRPGDVL
                     THAFRPFPNSPATAQGTVKKVVLEARERGVLFDIGHGKGSFAFKTARAMLANGFYPDT
                     ISSDVHALCIDGPAFDQVTTMSKFLCM"
     gene            complement(1593..2585)
                     /locus_tag="VGA15_13500"
     CDS             complement(1593..2585)
                     /locus_tag="VGA15_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9588744.1"
                     /translation="MSIRSLLATSLILSCLSVMPMRAAETVTSAVSASRTTTYKTVTV
                     NGLNIFYREAGPSNAPAILLLHGFPSSSRMFDTLMPLLADRYHLIAPDYPGFGNSEAP
                     SPETFSYTFDAIADIVDGFTQAIHLNRYALYLQNYGGPVGYRVAVAHPERVTALIIQN
                     AIAHEEGLGRLQAVRKGFWADRAANEDKYRAGLASLDVARLRHVNGSPHPERYNPDLW
                     KDEAAFLARPGEADIQSDLFYDFRNNLAAFPAWQNWLRERRPPTLIVWGRYDPVFDVA
                     EVTALRKDVPNAEVHLLDAGHFALDESASDVARLMRGFLGRLSHRAPKAISTHS"
     gene            complement(2751..3620)
                     /locus_tag="VGA15_13505"
     CDS             complement(2751..3620)
                     /locus_tag="VGA15_13505"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9588745.1"
                     /translation="MPTTIDQASPWLQGRTFASWREEFDSSGYLIFERVLSQDRVAGI
                     RAALAPYLERDLTGRNDFEGLKTNRVYALLAKSPLFAELAIHPLALAFAEAELGQSCL
                     LSALLAINLHPGETVQPWHFDDGGVRLPRPRPAFGLSAFWAIDDTTELNGSTEVIPGS
                     HLWNEQAVEGAVKPADFTNKAAPDADRDFGNRPDAVKITMPSGSLAVAKGTLWHRGGA
                     NLSDRPRLIITPQYCAGWVRQLENMVLAVPAEVAEKLPERARELIGYSIHPPFMGHVD
                     GVHPRRLLRSQAI"
     gene            3758..3904
                     /locus_tag="VGA15_13510"
     CDS             3758..3904
                     /locus_tag="VGA15_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588746.1"
                     /translation="MHRVWIEAALNGPWSRALQPGIPDTVEAIIAEGLACARRRHDHS
                     HPRL"
     gene            3885..4775
                     /locus_tag="VGA15_13515"
     CDS             3885..4775
                     /locus_tag="VGA15_13515"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX9588747.1"
                     /translation="MHTHAYDGGGPQTFDWQVYARIIEGIRAKVDVPVYPSYPAILTA
                     NIDASFAVADAAVRFAHIEALAARGLLEFAVIDPGSVNFTLTATMSAAKPAGTYLNPE
                     SHVRHALDFAARYGFHPAFAIYEPGFTRAGAALARAASVKTPIYRFMLSEQFAFGFPP
                     KPYAPAAHLALLEEEAGPAPWMIAGLGANNKAGHWPCRVPPRIQAAWHVENASRSIKV
                     RGDKKRSKRKINMPLVLRTVCRNRVPTHSSDPGLCNRLPGLIAGFAAAFAARRCASVG
                     PREKYRPAFDHLEGGRACDA"
     gene            4829..5122
                     /locus_tag="VGA15_13520"
     CDS             4829..5122
                     /locus_tag="VGA15_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588748.1"
                     /translation="MKLTSAQVERTINQFEAEALPDSHPAVRQLNELFGDHTFLLDSN
                     GLNILEPADEAPRDGVQAARVVNLANWSDETLTRLAPHEPEPTGAIIELESKH"
     gene            5188..5595
                     /locus_tag="VGA15_13525"
     CDS             5188..5595
                     /locus_tag="VGA15_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HEX9588749.1"
                     /translation="MRPLVLLVAFLIGLATPVRAADDIAAAQTIIRSQVEALGRDDAA
                     AAYAFAAPAIQDMFPQADIFMDMVRKSYAPVYRHKSFEFGEARVSDGQIAQRVHIIDA
                     DGVPWEALYTLELEPDGSVKISGCVLLKAGQAV"
     gene            5771..6148
                     /locus_tag="VGA15_13530"
     CDS             5771..6148
                     /locus_tag="VGA15_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9588750.1"
                     /translation="MKTVQVMLAEAEAQVPRISPEEAKGLLGRTDVLFLDVREPAEVV
                     ASGKVPGALAIPRGLVEFRADPASPLHDKAFDQAKTVVAYCASGGRSALVGKTLKEMG
                     YANVKNLGGFKGWLDAGGEVEKA"
     gene            complement(6231..7508)
                     /locus_tag="VGA15_13535"
     CDS             complement(6231..7508)
                     /locus_tag="VGA15_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9588751.1"
                     /translation="MLLDLQAAGDEVPDVHAPADSSKHSSLFTPDSGQAWVRLGLALI
                     IGSIGSVGMWSVVVVLPGVQAEFGATRGAVSLAFTLIMLGFGLGGVATGRITDRFGIV
                     LAMALSISFLGIAYILAGLSTTLWQFIAVHFLIGLGTSATFAPLMAEVSHWFERYRGL
                     AVGIVASGNYVGGTIWPPLVNWGTQATGWRTTHIAIGIFCAVTMTLVLLVLRARIGGA
                     TLRDHENAPPPRVDLRLSTNALTTLLCVAGISCCVAMAMPQVHIVAYCGDLGYGVARG
                     AEMLSLMMAFGVVSRIGSGFLADRIGGIRTLLIGSVAQGFALLFYLFFDGLTSLYVIS
                     AMFGLFQGGIVPSYAIIVRESMPAREAATRVGLVILASVFGMSFGGWVSGVIFDATGS
                     YAAAFINGLAWNALNIAIMVPLLIRARLRLALA"
     gene            7921..>8663
                     /locus_tag="VGA15_13540"
     CDS             7921..>8663
                     /locus_tag="VGA15_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEX9588752.1"
                     /translation="MVDITAGQGGATGNGFPSLSEIGTILKRGDLALAFGILTILVVL
                     ILPLPSIVLDLFLAISITLSILILMTSLFIQAPLEFSSFPTILLISTMLRLSLNLAST
                     RLILSRGHEGTAAAGHVIEAFGNFVMGGNFVIGIIVFAILVIVNFVVITKGSGRIAEV
                     AARFHLDAMPGKQMAIDADLSAGLIDEKVAKERRKALEDESGFFGAMDGASKFVRGDA
                     VAGLLVVFINVIGGIIIGVAQQGLGFGEAA"
BASE COUNT         1766 a         2682 c         2685 g         1530 t
ORIGIN      
        1 catgcacagg aacttggaca tggtcgtcac ctgatcgaag gccgggccgt cgatgcacag
       61 ggcatggacg tccgaggaga tggtgtccgg ataaaagccg ttggcgagca tcgcccgcgc
      121 ggtcttgaag gcgaacgagc ctttaccgtg gccgatgtcg aacaacacgc cgcgttcgcg
      181 cgcttccagc accacctttt tcaccgtgcc ctgggcggtg gcgggcgaat tgggaaacgg
      241 ccggaaggca tgggtgagaa catcgccagg acgcagccgg gcaagcacct cctcgtagct
      301 cggcggcgga tggtcgatat gcgccatcag cggcatgccg acctcctcgg cgacctcaag
      361 cgcgatttcg agcggcacaa tgcccgaggt gcccgaggag tgctggccga cccgcacctt
      421 gatgccgacg atgaggtcgc ggttgagctc cgccaccctg acggcgtcga tcggattcat
      481 caaccggatc tcctcactct cgccaaccat gatccgatgc gagaagccaa aaatgccggc
      541 gtgtgagaca tgcagatagg cgagaatgcg gacctggctc ggctcgatca cgtgcttgcg
      601 gaagcccgcg aaattgcccg ggccggcgct gccggagtcg accgccgtgg tgacgccgga
      661 ggtacggcag aattcctcgg cgtcgatacc gatcgaggtg ccgccccaat agacgtgggt
      721 atgcagatcg ataagaccgg gggtgacgat gtaacccgac acgtcgcgca cgtcggttcc
      781 cggatcaacg ctcagctcgt ttccgaccct gaccaccttg ccggcggcga aggcaacatc
      841 cgtcacggcg tcgagtttct gggaagggtc aatcacccga ccgccgcgca ggaccaaatc
      901 gaaaggcatt gtaatctcct gatgtgacag ggaaggaagc ggcggcggtg cgtcagtcgc
      961 gcaggatcac gcgctgccgc gctcggcgca tcggatcgag tatgctccgg acgacacgcg
     1021 cgcgatcacc gctcctttgg atcggcccga gatggccggc accaatggcg agaatgcggc
     1081 gcatgcgtcc atctttttgt gaaatgaaca tgtcgacgtc tctctcggcc gcgtcgtggt
     1141 ccggaagccg gtagcgcacg ggccgacgat aatgttagca agtttcgcgc caccaagccg
     1201 agaattgagg acgctgacgt gcacgtctgc ttttgcgcgc tcaacggcag acgtgaaggt
     1261 gcccagcccg gttcagcagc gacggaaagc tgcataggcg ggcgatctgc ggttcacaca
     1321 ggagcgcggc ctgttccgcg ccgatgatga ggggacgaca ccgcgcgatc atcttggcct
     1381 gcggcgaccg aacaacacct tgttccaggt ctgaagccga cttccgagga agacgactac
     1441 tttgcgccga tactgctgct gaaagtgtct gctttggctg aggaacgaaa tttttcagcc
     1501 ggcccggtgc gtaccgcgcg tgtcgacgcg agggaccata tcgattcaca gaaaagcgat
     1561 cacagaccct cggccgcgcg gctcgcccgc cttcaactat gcgtgctgat cgccttcggc
     1621 gcacggtgcg acagtcgccc caaaaagcca cgcatcagcc gggctacatc gctcgcggac
     1681 tcatcgagcg cgaaatgtcc ggcatccagc agatgcacct ccgcgttcgg cacgtctttc
     1741 ctgagcgcag tgacctcggc aacgtcgaac accgggtcgt agcggcccca gacgatcagg
     1801 gtgggcggcc ggcgttcgcg taaccaattc tgccacgccg ggaaggcggc aagattgttg
     1861 cggaagtcgt agaacaggtc cgactggata tcggcctcgc cgggcctcgc caggaacgcg
     1921 gcttcgtcct tccagagatc cgggttgtag cgctcggggt gcggcgagcc gttcacgtgc
     1981 cgaagccggg ccacgtcgag cgaggcaagc cctgcacggt acttgtcctc gttggcggcc
     2041 cggtccgccc aaaaaccttt gcgaacggcc tgcagtcggc cgagcccctc ttcgtgcgcg
     2101 attgcattct ggatgatcag cgcggtcacc cgctccggat gcgcgacggc gacccggtag
     2161 ccgaccggtc cgccgtagtt ctgcagatag agcgcgtaac ggttcagatg gatcgcttgc
     2221 gtgaacccgt cgacgatgtc ggcgatcgca tcgaaggtat aggaaaacgt ctccggcgag
     2281 ggcgcctccg agtttccgaa gccgggataa tcaggcgcga tcaggtgata gcgatcggcg
     2341 agcaagggca tcagcgtatc gaacatccgg gacgatgacg ggaagccatg cagcagcagg
     2401 atggccggcg cgttgctcgg gcccgcctct cgataaaaga tgttcaggcc gttgacggtg
     2461 acggttttgt aggtcgtcgt tcgactggct gatacggctg atgtgacggt ctcggcggcc
     2521 ctcatcggca tcaccgacaa gcagctcaaa atgagcgagg tggcgagcag gctgcgaatg
     2581 gacatgagaa tctccgaaaa gccgcggttc gtccgcttgg cagtgaacgc gatcagcctt
     2641 caaaggtggc cgaaaccctg ttgcagaata tgtggggatg gcagcatcgc ccgcgtcata
     2701 cgttcgaatg agaacacgca aggagtcaca cgcataaagc gcctgtccca tcatatcgcc
     2761 tgcgaccgca acaatcgcct ggggtgaacg ccatcgacgt gccccatgaa cggcggatgg
     2821 atcgaatagc cgatcagttc gcgggcacgc tccggcagtt tttccgccac ctcagccgga
     2881 acggcgagga ccatattctc gagctgccgc acccagccgg cgcaatattg cggcgtaatg
     2941 atcaagcgcg gccgatccga cagattagcg ccgccccggt gccagagcgt tcccttcgcc
     3001 accgccagcg atccggatgg catcgtgatc ttcaccgcat ccggccggtt gccgaaatcg
     3061 cgatcggcat ccggcgcagc cttgttggta aagtcggccg gcttcaccgc gccctcgacg
     3121 gcctgctcgt tccaaaggtg gcttcccgga atcacttcgg tgctgccatt caattcggtg
     3181 gtgtcgtcga tcgcccagaa cgcgctgaga ccaaacgccg gacgcggccg gggcagcctg
     3241 acgccaccgt cgtcaaaatg ccagggctgc accgtttcgc cgggatggag gttgatcgca
     3301 agcagcgccg acagcagaca gctttgtccc agttccgctt cggcaaaagc cagcgccagc
     3361 gggtggatcg caagctctgc gaacagcggc gacttcgcca acaaggcata tacccggttg
     3421 gtcttgaggc cctcgaaatc gttgcggccg gtgaggtcac gctcaaggta cggcgccaac
     3481 gcggcgcgga ttccggctac acgatcctgc gaaagaacac gttcgaagat aaggtagccg
     3541 ctagaatcga actcttcccg ccacgatgcg aaggttcgtc cctgcagcca gggcgatgcc
     3601 tgatcgatcg ttgtcggcat tggctttctc ctccccatca aacaaattgt ttaaatgctg
     3661 gcgtgatcgg gccgatgatc cccagaggat acgatgaacc caacagcctg tctaatcgag
     3721 acaagcacgt gagcgaaaac gaaaaggagg cgcggcgatg cacagggtct ggattgaagc
     3781 ggcattgaac ggtccctgga gccgcgcgct gcaacctggc attcccgaca ccgtcgaggc
     3841 gatcatcgcg gaaggcctcg cctgcgcgcg ccggcgccac gatcattcac acccacgcct
     3901 atgacggcgg cggcccgcag actttcgact ggcaggttta cgcgcgcatc atcgagggta
     3961 ttcgcgcgaa ggtggacgtt ccggtttatc cgtcttaccc agcgatcctg acagccaaca
     4021 tcgatgcaag ttttgcagtc gcggatgccg cggttcgatt cgcccatatc gaagcgctcg
     4081 ccgcgcgcgg tctgctggaa tttgcggtga tcgaccccgg cagcgtcaat ttcacgctaa
     4141 ccgcaacgat gtctgcagcg aaaccggctg gcacctacct gaatccggaa agccatgtcc
     4201 ggcatgcgct cgactttgcc gcgcgctacg gctttcatcc ggccttcgcg atctacgaac
     4261 cggggttcac gcgcgccggc gcagcccttg cgcgcgcggc cagcgtcaaa actccaatct
     4321 accgtttcat gctctcggaa caatttgcct tcggcttccc gcccaagccc tatgccccgg
     4381 cggcgcatct ggcgctgctc gaagaagaag ccggcccggc gccatggatg atcgcggggc
     4441 tgggcgcaaa caataaagcg ggccactggc cgtgccgggt tccccccaga attcaggcgg
     4501 cctggcatgt cgagaacgca tctcgatcaa ttaaggttcg cggagacaag aaacggtcaa
     4561 aaaggaagat caatatgccg ctggtattgc ggacggtatg ccgcaatcga gtccccacgc
     4621 acagctccga ccccggcttg tgcaatcgat tgccgggttt gattgcaggg ttcgccgctg
     4681 ctttcgctgc tcgaagatgc gccagcgtgg gtccgcgcga gaaatatcga ccagcgttcg
     4741 accaccttga aggtggcagg gcatgcgacg catgacccgc aacgccccgg cgttgcaaca
     4801 cgaagaaacc agttgaaagg aataagcgat gaaattgact tccgcacagg tcgagcgaac
     4861 catcaaccag ttcgaagccg aagcccttcc cgatagccac ccagcggttc ggcaactgaa
     4921 cgaattgttt ggggatcaca cttttttgct cgatagcaat ggactgaaca ttttggagcc
     4981 ggccgacgag gctcctcggg atggagttca ggcggcgaga gtcgtcaatc ttgcgaattg
     5041 gagcgacgag actctcacgc gcctggcgcc tcacgaaccc gagccgactg gcgccatcat
     5101 cgaactggaa tccaaacact gattttagat ggcgctttag gcgcgctccg gtaatcttgt
     5161 agcctggcaa gagcacccgg agttctcatg cgcccactcg tcttgctcgt cgccttcttg
     5221 atcggtctcg cgacacccgt ccgcgccgcg gatgatatcg ccgccgcgca gaccatcatc
     5281 cgttcccagg tcgaagcgct cggccgcgac gatgcggcgg cggcctacgc ttttgcggcg
     5341 cccgctatcc aggacatgtt tccgcaggcc gacatcttca tggacatggt gcgcaagagc
     5401 tatgccccgg tctatcgcca caagagcttt gagttcggcg aggcgcgcgt ctcggacggc
     5461 cagattgccc agcgcgttca catcatcgac gccgatggcg tgccgtggga ggcgctgtat
     5521 acgctggagc tggagcccga cggcagcgtg aaaatcagcg gctgtgtttt gctcaaggcg
     5581 ggccaggcgg tataaagcgt tttcgagcga aagcctgccc cgcacttgat gcggggtgga
     5641 tgcgcgttta gagcatgatc cggaaaagtg gaaaccggtt ttccgaaaag atcatgctca
     5701 aacaaagaga tgagatcatg atccgattca agccaatcgg atcatgatct ggtgcgagag
     5761 gagacttaaa atgaaaacag tccaggtgat gttggccgag gccgaagcac aggtgccgcg
     5821 tatcagccct gaggaagcaa aagggctgct tggccgaacc gacgtattgt ttctcgatgt
     5881 ccgcgaaccg gccgaggtag tggcatcggg aaaagtcccg ggcgcccttg caatcccgag
     5941 aggacttgtg gaatttcgcg ccgatccggc ttcgccgctg cacgacaaag cgtttgatca
     6001 ggccaagacg gtggttgcct attgcgcgtc aggtggacgc tcggcgctgg tcggcaagac
     6061 gctgaaggag atgggatacg ccaacgtcaa aaaccttggc ggcttcaaag gctggctgga
     6121 tgcaggcggc gaggtcgaaa aggcataatt gacgctccgt cctctgcggt cgtcattcgg
     6181 cgcgatcaga acgagctgtt gcggcgcgcg cgttctgtca aaggcaggcc ttacgccagc
     6241 gccagccgca gccgggcgcg gatcaacagc ggcaccatga tggcgatgtt gagcgcgttc
     6301 cacgccaggc cgttgataaa tgccgcggcg taggagccgg tggcatcgaa gatcacgccg
     6361 gacacccagc cgccgaacga catgccgaac accgaagcca aaatcacgag gccgacgcgg
     6421 gtcgcagctt ctcgcgccgg catcgattcc cggacgatga tggcgtagct tggcacgatg
     6481 ccgccctgaa acaggccgaa catcgcggag atgacataga gcgaggtcag gccgtcgaaa
     6541 aacagataga acaacagcgc aaagccctgc gcgaccgagc cgatcagcaa ggtgcggatg
     6601 ccgccgatcc tatcggcgag gaaccccgat ccgatccggc tgacgacgcc gaacgccatc
     6661 atcagcgaca gcatctcggc gccgcgcgcg acgccatagc cgagatcgcc gcaataggcg
     6721 acgatgtgga cctgcggcat cgccatcgcc acgcagcagg aaatgccggc gacgcacaat
     6781 agtgtcgtca gcgcattggt cgagagcctg agatccacgc gcggcggcgg cgcgttctca
     6841 tgatctcgca gcgtcgcccc gccgatgcgg gcgcgaagca ccagcagcac gagcgtcatc
     6901 gtcaccgcgc agaaaatgcc gatggcgatg tgggtggtcc gccagccggt agcttgcgtt
     6961 ccccaattga ccagcggcgg ccagatcgtg ccgccgacgt agttgccgct tgcgacaatg
     7021 cccaccgcga gcccgcgata gcgttcgaac caatgcgaga cctccgccat cagcggcgcg
     7081 aaggtcgccg atgtcccgag cccgatcagg aaatgcacgg caatgaactg ccacagcgtg
     7141 gtcgaaagtc cggcgagaat ataggcgatc ccgaggaagc tgatgctgag cgccatcgcc
     7201 aatacgatgc cgaagcgatc ggtgatccgc cccgtcgcga cgccgccgag accgaagccg
     7261 agcatgatca gcgtaaaggc cagcgatacc gcgccgcgag tcgcgccgaa ttcggcctgc
     7321 acccccggca gcaccaccac gaccgaccac atcccgacgc tgccgatcga gccgatgatg
     7381 agcgcaagcc caagcctcac ccatgcctga ccggagtccg gggtgaagag agacgaatgt
     7441 ttcgatgaat cagcgggggc gtgcacatcg ggaacttcgt cgcccgctgc ctgaaggtca
     7501 agcagcatgg cgcgatatta ggcatgcaga tgcgcagcaa cagaacagcc cttttcacga
     7561 cccctcgccc cttcgtcatt ccgggatggg ccgaaggccc aggcctcaga tgtgcaattg
     7621 cacatcgggg aatctcgaga ttccgcgttc gacgccttgc gtctccccgg aatgacggaa
     7681 tgtcgaaacg ccctctcaca cctctcgtcg cacgcgggga gagggacaag gccgttaacg
     7741 ctttcctaac cataaagcgg gcaaaaattg cctagtggag tttgagtgtc gtcagccgcg
     7801 taagacggag gagtacccgt ggacgccagt gcggtgccta actttcgaaa cggtggtcct
     7861 tcggccgccg ccaagacgtt tggcgcccgc gaccggcata tgcgggggga agcagcaacc
     7921 atggtcgaca tcacggcagg tcagggcgga gcgaccggca acggatttcc gagtctcagc
     7981 gaaatcggca ccatcttaaa gcgcggcgat ctcgcgctgg cgttcggcat cctcaccatc
     8041 ctggtggtgc tgatcctccc tctgccctcg atcgtgctcg atctgtttct cgcgatctcg
     8101 atcaccctgt cgatcctgat cctgatgacg tcgttgttca tccaggcgcc actggaattc
     8161 tcgtcgttcc cgaccatcct tctgatttcg acgatgctgc ggctttcgct caacctcgct
     8221 tccacgcggc tgatcctgtc gcgcggacat gagggtaccg ccgccgccgg ccacgtcatc
     8281 gaagccttcg gcaatttcgt gatgggcggc aatttcgtga tcgggattat cgtcttcgcg
     8341 atcctggtga tcgtgaattt cgtcgtcatc accaagggtt cggggcgcat cgcggaagtc
     8401 gccgcccgct ttcacctcga cgccatgccg ggcaagcaga tggcgatcga cgcggacctc
     8461 tccgccggct tgatcgacga gaaggtcgct aaagagcgcc gcaaggcgct ggaagacgaa
     8521 agcggcttct tcggtgcgat ggacggcgcg tcgaaattcg tgcgcggcga cgcggtcgcg
     8581 ggtctgctcg tcgtgttcat caacgtcatc ggcggcatca ttatcggcgt cgcccagcag
     8641 ggcctcgggt tcggcgaagc cgc
//
LOCUS       DASWGM010000469         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2546696,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000469 DASWGM010000000
VERSION     DASWGM010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2546696"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /locus_tag="VGA15_13545"
     CDS             complement(<1..1956)
                     /locus_tag="VGA15_13545"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9588753.1"
                     /translation="MNHRTSRGGGYGRETGIIDLGHEPPLSVDGSEAAVIDRRRVSVQ
                     WFSGTILTGLCGAALIGGAVFASLDGEMTFAKVPERVEGALRGAFGANDRTASLRKSD
                     RLPPPSESAAARNVMRVSTVTRVGNRDVMRVRPFIRISGNLSMATSDLSAKIPPFNAQ
                     RMLTDVGAATPAASDDPNNADAVEPDAEVSFVTKDLAPVLPKAKLAAVVALDDVLMRV
                     RDASNWRGGGSGVRYAALANATADASGQSDLKLNYATEGTVADPYAGFETRVVPENVT
                     LLPKTKEQATGGNPSNERVHVVKKGDTVTSILRDQGATPEEARSIATTLGPRGRDGGL
                     KEGEKLRILMAPAGPGQRLQPYRVIVANDSTIEAVAALSDLGKYVAVDVQSMNTVTET
                     TDSSEDDDDDGTGVRLYQSIYETAMRNKVPAAIIEDMVRIYSYDVDFQRKVQPGDSFD
                     VFFAGEDEGAAITEKNDVLFASLTVGGETKKYYRFQTPDDSVVDYYDETGKSAKKFLV
                     RKPVNDAIMRSGFGSRRHPILGYVKMHTGVDWATAYGTPIFASGNGVVEVAGLEGGYG
                     KYVKLKHNNGYETAYGHMSAFAKGLEVGKRVRQGQLIGFVGSTGQSTGPHVHYEILVN
                     GRFVDPMRIKLPRGRSLDGPLMAGFEKE"
BASE COUNT          428 a          764 c          802 g          475 t
ORIGIN      
        1 ctccttctcg aagcccgcca tcagcgggcc gtcgagcgag cggccgcgcg gcagcttgat
       61 gcgcatcgga tcgacgaagc ggccgttgac cagaatttca taatggacat ggggcccggt
      121 cgactgcccg gtcgaaccga cgaagccgat cagctggccc tgccgcaccc gtttgccgac
      181 ctcgagcccc ttggcgaacg ccgacatgtg gccgtaggcg gtctcgtaac cgttgttgtg
      241 cttcagcttg acgtatttgc cgtagccgcc ttcgaggccg gcgacttcga cgacgccgtt
      301 gccggatgca aaaatcggcg tgccgtaggc ggtggcccag tcgacgccgg tatgcatctt
      361 gacgtaaccg agaatgggat ggcggcggct gccgaagccc gagcgcatga tcgcatcgtt
      421 gaccggcttg cgtaccagga actttttcgc gctcttgccg gtctcgtcgt agtagtcgac
      481 gacggaatcg tcgggggtct ggaaacggta gtatttcttg gtttcgccgc cgacggtgag
      541 ggaagcaaac aacacgtcgt ttttctcggt gatggcggcg ccttcgtctt cgccggcaaa
      601 gaatacatcg aaggaatcgc cgggctgcac cttgcgctgg aagtcgacgt cgtaggagta
      661 gatgcggacc atgtcctcga tgatggcggc cggcaccttg ttgcgcatcg cggtctcgta
      721 gatgctttga tagagccgca cgccggttcc atcgtcatca tcatcttcgc tgctgtcggt
      781 ggtctcggtg acggtgttca tgctctgcac atcgaccgcg acgtatttgc cgagatcgga
      841 cagcgcggcc acggcttcga tcgtggaatc attggcgacg atcacccgat agggctgcag
      901 ccgctggccg gggcctgccg gcgccatcag gatgcgcagc ttctcgcctt ccttcagtcc
      961 gccgtcgcgg ccgcgggggc cgagcgtggt ggcgatggat ctcgcctctt ccggcgtggc
     1021 gccctgatcg cgcaggatcg acgtgacggt atcgcccttc ttgaccacgt gaacgcgttc
     1081 gttggacggg ttgccgccgg tggcctgttc tttcgttttc ggcagcagcg taacgttttc
     1141 cggcacgacc cgggtttcaa agccggcata ggggtcggcg acggtgccct ctgtggcgta
     1201 gttcagcttg aggtcggact gcccgctggc gtcggccgtc gcgttggcga gggcagcata
     1261 gcgcacgcca ctgccgccgc cgcgccaatt ggaggcatcg cgcacccgca tcagaacatc
     1321 gtcgagcgcg accaccgcgg cgagcttcgc cttcggcaga accggcgcaa ggtccttcgt
     1381 gacgaatgaa acctcggcat cgggctcgac ggcgtcggcg ttattgggat cgtccgaggc
     1441 ggcgggagtg gcggcgccga cgtcggtcag catgcgctgg gcgttgaacg gcgggatctt
     1501 ggcgctgaga tcgcttgttg ccatcgacag attgccggag atgcggatga acggccgcac
     1561 ccgcatcacg tcacggttgc cgacgcgggt gacggtcgag acccgcatca cattgcgcgc
     1621 cgcggccgac tcgctgggcg gcggcagacg atcgctcttg cgcaggctgg cggtgcggtc
     1681 gttggcgccg aacgcgccgc gcagcgcgcc ctcgacgcgc tccggcacct tggcgaacgt
     1741 catttcgccg tcgagcgacg caaaaacggc gccgccgatc agagctgcgc cgcacagacc
     1801 cgtcaggatg gtgccgctaa accattgcac ggagacgcgg cggcgatcga tcaccgcggc
     1861 ttcggagcca tcgacggaaa gtggcggctc gtgaccaaga tcaatgatcc cggtctcgcg
     1921 tccgtaaccg cccccgcgtg acgtcctgtg attcaaccca ccgtcccccc gaattccagc
     1981 agcattcgac gagaccttgg cccatcctga gcccgcattc gccctgtgag ggcgatccgg
     2041 gggcagggcc gtgccacatg gctgctcggg aaatcaaagg ccaaaaggct ggccggaatc
     2101 tcgaacaaca atggtatgaa gatctctcga tgtctctcgg ccgaaaggga acaacgacgg
     2161 gctcatggaa gatcgccgat ccctcaaaga agttgaatac cggcagcccc tactgacgcc
     2221 cggcgattca atgcttagca taacgccaga acgtcgcagg attgtggctc aagtacggcg
     2281 cgatgccctt taatggaggc ttcttggcgg ccccagtttc tgttggatga tgcgacgatt
     2341 tgttgacaac agccgttgac aacgatgatc gggcgggctt ataacccggc cactgagcgc
     2401 ggcgctgtct tggcccttgg ccaaggcaag ttttcgtgcc cgtgaagctc ctcactaaga
     2461 tgagtgaat
//
LOCUS       DASWGM010000470         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1556112,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000470 DASWGM010000000
VERSION     DASWGM010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556112"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VGA15_13550"
     CDS             complement(<1..365)
                     /locus_tag="VGA15_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9588754.1"
                     /translation="MNFRAVRAIYLFEMARTWRTLLQSIVSPVVSTSLYFVVFGAAIG
                     SRITSVEGVSYGTFIVPGLVMLSVLTQSIANASFGIYFPKFVGTIYELLSAPISYFEI
                     VLGYVGAAATKSIILGLIIL"
     gene            complement(362..1285)
                     /locus_tag="VGA15_13555"
     CDS             complement(362..1285)
                     /locus_tag="VGA15_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588755.1"
                     /translation="MPSIISVSNLSKTYGSGFKALNGINLEINRGEIFALLGPNGAGK
                     TTLISIICGIANLSEGNVIVGGHDINKEYRAARSLIGLVPQELHTDAFETVWATVSFS
                     RGLFGKPKNPAHIEKVLKDLSLWDKKDSRIVTLSGGMKRRVMIAKALSHEPQILFLDE
                     PTAGVDVELRKGMWEVVRTLQASGVTIILTTHYIEEAEEMADRIGVINKGEIVLVEDK
                     AGLMQKLGKKRLKLHLQGKIDAIPADVAAYDLELCDGGHELIYTYDTKGERTGITSLL
                     SDLRKAGIRFYDLDTTQSSLEDIFVSLVRAS"
     gene            complement(1423..1713)
                     /locus_tag="VGA15_13560"
     CDS             complement(1423..1713)
                     /locus_tag="VGA15_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9588756.1"
                     /translation="MAHYIAIIEDAGPDHAVGVWFPDLPGCTSAGDDIDQALRNAPEA
                     LELYAESFQTDGKPLPRPRTLTELKADPEIADDIKTYMVALIEFPVRAHAAE"
     gene            complement(1722..1916)
                     /locus_tag="VGA15_13565"
     CDS             complement(1722..1916)
                     /locus_tag="VGA15_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9588757.1"
                     /translation="MLTNSRDIIRRLEREGWTLVRVTGSHHVLKSPISGATVVVPHPK
                     KDFGKGLVRAIYKGAGWEPD"
     gene            complement(1986..>2478)
                     /locus_tag="VGA15_13570"
     CDS             complement(1986..>2478)
                     /locus_tag="VGA15_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9588758.1"
                     /translation="LDELPAHNVEITESKGGSGWGGASGYGPSRFDNVESFGSSYSTP
                     GWQRAQANRARNQGGRNGQSGQAGGGFNESQSPFSGSRSDASARSDAGGFARNKRGPM
                     TIEGELVAKSTGTTSEFSLDDRVFHQKFGYGNVVKVDGNKLTIAFEKAGEKKVVDSFV
                     ERV"
BASE COUNT          494 a          734 c          744 g          506 t
ORIGIN      
        1 agaatgatca ggccgaggat gatcgatttg gtcgcggccg cgccgacata gccgagcacg
       61 atctcgaaat acgagatcgg cgccgacagc aattcgtaga tcgtgccgac gaatttcgga
      121 aagtagatgc cgaacgaggc attggcgatg ctctgcgtca gcaccgacag catcaccagc
      181 ccgggcacga tgaaggtgcc gtagctgacg ccttcgaccg aagtgatgcg cgagccgatc
      241 gccgcaccaa acaccacgaa atacagcgac gtcgaaacca ccggcgaaac gatgctttgc
      301 agcagcgtgc gccaggtgcg cgccatttcg aacagataga tcgcgcggac cgccctgaaa
      361 ttcatgacgc cctcaccagg ctgacgaaga tgtcttccag cgaactctgg gtggtgtcga
      421 gatcgtagaa gcggatgccg gccttgcgca gatcgctgag caggctggtg atgccggtgc
      481 gttcgccctt ggtgtcgtag gtgtaaatca gctcgtgtcc cccgtcgcat agttcgagat
      541 cataggccgc gacatcggcg gggatcgcat cgatcttgcc ctgcagatgc agcttcagcc
      601 gcttcttgcc gagcttttgc atgaggcccg ctttgtcctc gaccaggacg atctcgccct
      661 tgttgatgac accgatgcgg tcggccatct cctcggcctc ttcgatgtaa tgcgtggtga
      721 ggatgatggt gacgccggag gcctgcagtg tccgcaccac ttcccacatg cccttgcgca
      781 attcgacatc gacgcccgcg gtcggctcgt cgagaaacag gatctgcggc tcgtgcgaca
      841 gcgcttttgc gatcatcacg cggcgcttca tgccgccgga cagcgtgacg atcctggaat
      901 ccttcttgtc ccacagcgac aggtccttca gcaccttttc gatatgcgcg gggtttttcg
      961 gcttgccgaa caggccgcgg ctgaaactga cggtcgccca caccgtctcg aaggcgtcgg
     1021 tgtgcagttc ctgcggcacc agcccgatca gcgagcgcgc ggcgcgatat tccttgttga
     1081 tgtcgtggcc gccgacgatg acgtttcctt cgctgagatt ggcgatgccg caaatgatgc
     1141 tgatcagcgt ggtcttgccg gcgccgttcg gccccagcag cgcaaagatc tcgccgcggt
     1201 tgatctccag attgatgcca ttcagcgcct tgaaaccgga gccgtaggtc ttggacagat
     1261 tggagacgga tataatggaa ggcatgacgg ctgtgatgat ctgaactgga aatgccgaga
     1321 aggattaaag ccccgcgggg gccggtcgcg gctagggagt ggcgagcacc aacagatagg
     1381 aacgctcaga gcattgtgca atcggtcagg cagacaatcg agtcattccg ccgcgtgagc
     1441 ccgaacggga aattcgatca gtgcaaccat gtaggtcttg atgtcgtctg cgatttccgg
     1501 atcggctttc aattcagtca aggttcgtgg gcgaggcaac ggtttgccat ccgtttgaaa
     1561 gctttcggcg tacagttcca aagcctctgg tgcattgcgc aatgcctgat caatatcatc
     1621 gcccgcggat gtgcagccgg gtagatccgg gaaccaaacg ccaacggcgt gatccggtcc
     1681 tgcatcctca atgatagcga tgtaatgtgc cacggcggac ctcaatcagg ctcccatcct
     1741 gcgcccttat aaatggcacg aaccaggcct tttccaaagt ccttcttggg gtgtggaaca
     1801 accacggttg cgccagaaat gggacttttg agaacgtggt gggagccggt gacccgaaca
     1861 agtgtccatc cctcccgctc aagacggcga atgatgtcgc ggctgttggt caacataagg
     1921 cgcctcccat cgaatcatat gatggaagga tggcgccggc tagcccgcgt tgagcgcggg
     1981 gaaaactaca cccgctcgac gaaactatcg accacctttt tctcgccggc cttttcgaag
     2041 gcgatggtga gcttgttgcc gtcgaccttc acgacgttac cgtaaccgaa tttctggtgg
     2101 aacacgcggt cgtccaacga aaactccgat gtggtgccgg tggatttcgc gaccaactcg
     2161 ccttcgatgg tcatcggacc gcgcttattg cgtgcgaagc cgccagcgtc gctgcgcgcg
     2221 gaggcatcgc ttcgcgagcc tgagaatggc gactggctct cattaaaacc gccgccggcc
     2281 tggccgctct gtccattgcg gccgccctgg ttgcgcgcgc gattggcttg cgcccgttgc
     2341 cagcccggcg tcgaatagct cgagccgaac gattccacgt tgtcgaagcg cgaggggccg
     2401 tagccgctgg caccgcccca gcccgaaccg cccttggatt cggtgatctc gacattgtgc
     2461 gccggcaatt cgtcgaga
//
LOCUS       DASWGM010000471         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2547001,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000471 DASWGM010000000
VERSION     DASWGM010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2547001"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VGA15_13575"
     CDS             <1..1192
                     /locus_tag="VGA15_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX9588759.1"
                     /translation="AYFAQFELDVPCVAMVTASHNDNGWTGVKMGANRPLTFGPDEMT
                     RLKEIVLNADFKNKVGGSYQFHENFPARYIADLTKRPKLKRKLKVVVACGNGTAGAFA
                     PQVMEAIGCEVVPLDTELDYTFPKYNPNPEDMEMLHAIRDAVLEHKADVGLGFDGDGD
                     RCGVVDNSGEEIFADKVGVMLARDMSAITKDAQFVVDVKSTGLFVTDPVLQKQGAKVT
                     YWKTGHSYMKRRTNELGALAGFEKSGHFFFNPPLGRGYDDGLISAIAICEMLDRAPGK
                     SMADLKNALPKTWSSPTMSPHCPDEAKYGIAAQVVKHFEAMQSKGEKVAGQPIRDLVT
                     VNGVRVTVEDGSWGLVRASSNKPELVVVVESPVSEQRMRDLFEAMDSVLRTHPEVGEY
                     NQKI"
     gene            complement(1328..2797)
                     /locus_tag="VGA15_13580"
     CDS             complement(1328..2797)
                     /locus_tag="VGA15_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9588760.1"
                     /translation="MNSGSKDLDALRETASKALISLLWLHVPIAAVIGMARGTDWLMP
                     IILMIAFAAAATLSWRSVGNGLSTHLVIAVALMGGVSVFTFQLSGHAWQVDSHMYFFA
                     ALASLAAYCDYRPIIAGTIAVALHHLALNFILPAAVYPGGADFGRVVLHAVILLIEAG
                     VLIWLARKLSHLFETTTQKTAEAEAANAAEARANANRAEADLKAKQERDAAMRELAAG
                     FERKVGHIVEAVAVAAREMQGMSSSMSNSSGEAARQTAAAASASTQASLNVGTVASAA
                     EELTASISEIAHQATRSASVTGKAADEARRTNTVVEGLLTGTQKIGEVVTLIQNIASQ
                     TNLLALNATIEAARAGEHGRGFAVVASEVKALANQTAKATEEISTQIQSIQTATGEAV
                     SAIQAIGGTIAEINQISNAIAQAVGQQGTATREISGNVQQAANGTREVNDNIVGVAKA
                     SGELGSSASKLLEAANGLSSQSERLKREVDSFLGSVRAA"
BASE COUNT          539 a          876 c          913 g          556 t
ORIGIN      
        1 ggcgtatttc gcgcaattcg aactcgatgt gccctgcgtc gcgatggtga cggcatcgca
       61 taacgacaat ggctggaccg gcgtcaagat gggcgccaat cggccgctca ccttcggccc
      121 cgacgagatg acccgcctga aagagatcgt gctcaatgcc gacttcaaga acaaggtcgg
      181 tggctcatat caattccatg agaattttcc ggcgcgctat atcgccgacc tgaccaaacg
      241 tccaaaactg aagcgcaagc tgaaggtcgt ggtcgcctgc ggcaacggca ccgcgggagc
      301 attcgcgccg caggtcatgg aggcgatcgg ctgcgaggtg gtgccgctcg ataccgagct
      361 cgactacacc tttccgaaat acaatcccaa tcccgaagac atggaaatgc tgcacgccat
      421 ccgcgacgcg gtgctggagc acaaggccga tgtcggcctc ggctttgatg gcgacggcga
      481 ccgttgcggc gtggtcgaca attcaggcga ggagattttc gccgacaagg tcggcgtgat
      541 gctggcgcgg gatatgtccg ctatcaccaa ggatgcgcag ttcgtggtcg acgtgaagtc
      601 gaccggactt tttgttacag atccggtgct gcaaaagcag ggcgccaaag taacctattg
      661 gaagaccggt cactcctaca tgaagcgccg caccaatgaa ttgggcgcgc tggcggggtt
      721 cgagaaatcc ggccacttct tcttcaaccc gccgctcggc cggggctatg acgacggcct
      781 gatttcggcg attgcgatct gcgagatgct tgatcgtgcg cccggcaagt cgatggcgga
      841 tttgaaaaac gcgctgccga agacctggtc gtcgccgacg atgtcgccgc attgcccgga
      901 cgaagccaaa tacggcatcg cggcgcaagt ggtgaagcat ttcgaagcga tgcagagcaa
      961 aggcgagaag gtcgcgggcc agccgatccg cgatctcgtc accgtcaatg gcgtgcgtgt
     1021 cacggtcgag gacggcagct ggggcctggt gcgggcgtca tccaacaagc cggaactggt
     1081 ggtggtggtc gagagtccgg tcagcgagca gcgcatgcgc gacctgttcg aggcgatgga
     1141 ttcggtgctg cgcacccatc ccgaggtcgg cgaatataat cagaagattt gactcgtcat
     1201 tgccgggctt gacccggcaa tccatccgtt tcgaaaatgc atttcgtgaa gacggatgga
     1261 tacgcgggtc aagcccgcgt atgacggccg tgaatgtcga tgcactgcgc gtgcatactc
     1321 ttccagccta cgccgcgcgg acggatccca ggaaactgtc gacctcccgt ttcaaccgtt
     1381 cggattgcga tgacagtccg tttgcggcct cgagcagctt cgaggcggag ctgccgagtt
     1441 cgccggaagc cttggctacc ccgacaatgt tgtcattgac ctctcgggtg ccgttcgccg
     1501 cctgctgcac gttgccggag atctcgcgtg tcgccgttcc ctgctgtccg acggcttgcg
     1561 cgatggcatt ggagatttgg ttgatttcgg cgatggtccc gccaatcgcc tggatcgcgc
     1621 tgacggcttc gccggtcgcg gtctgaatgc tctggatctg cgtcgaaatt tcctccgtcg
     1681 ccttcgcggt ctgattggcc agcgccttga cttcgctcgc cacaacagcg aatccccggc
     1741 cgtgctcgcc ggcccgcgcc gcttcgatcg tggcgttcaa ggcgagcaga ttggtctggc
     1801 tggcgatgtt ctggatcagc gtcacgacct caccgatctt ctgcgtaccg gtgagcagtc
     1861 cctcgacaac ggtgttggtg cggcgggcct cgtcggccgc ctttcctgta acgctggcgg
     1921 agcgggtcgc ctggtgcgcg atctcgctaa tcgaggcggt cagctcctcg gcagccgagg
     1981 ccacggttcc gacattgagc gaagcctgtg tcgatgcgga ggcggccgcg gccgtctgac
     2041 gcgcggcttc cccgctgctg ttgctcatcg atgaggacat gccctgcatt tcgcgagccg
     2101 ccacggcgac ggcctcgacg atatgcccga ccttgcgctc gaagcccgca gcgagttcgc
     2161 gcatcgccgc gtcacgttcc tgcttggctt tcaggtccgc ctcggcccgg ttggcgttgg
     2221 cgcgtgcctc ggcggcgttt gcagcttccg cctcggcggt cttctgcgtt gtcgtctcga
     2281 acaggtgtga gagcttgcgg gcaagccaga tcaatacgcc ggcctcgata aggagaatga
     2341 cggcgtgaag cacgacgcgc ccgaaatcgg cgccgccggg ataaactgcc gccggcagga
     2401 tgaaattgag cgccagatga tgcagcgcga ccgcgattgt tccggcgatg atcggccgat
     2461 aatcgcaata ggcggccagg gacgccaggg ccgcgaagaa gtacatgtgc gagtcgacct
     2521 gccaggcatg tcccgacaac tggaacgtaa acaccgaaac tccgcccatc agcgcgaccg
     2581 cgatcacgag atgggtcgaa agcccgttgc ccacgctgcg ccaggacaag gtggccgccg
     2641 cggcaaatgc gatcattaga atgatcggca tcagccaatc ggtgccgcgc gccatgccaa
     2701 tgaccgctgc gatcggaaca tgcaaccaca gcaacgagat caacgccttg ctggcggttt
     2761 cgcgcaaagc gtccaggtcc ttgctaccgc tattcatgct cagatttcct tgtcaatgtt
     2821 gaagcagccg gcaatggctt gggggtcgat ggtcagccat gcgccggcct cacgcaaacg
     2881 ggtc
//
LOCUS       DASWGM010000472         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1990806,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000472 DASWGM010000000
VERSION     DASWGM010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990806"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VGA15_13585"
     CDS             complement(<1..447)
                     /locus_tag="VGA15_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588761.1"
                     /translation="MKRCVARCLSGILLVLAVHLAASDVSCAQQPKKGGTLVYASVSG
                     PGTLDPYVASSSVELEVINNVFEGLVTLDANNATRTMLASKATASEDFKTYSFELRKG
                     VKFHNGQEMTSADVKASFERYQRVSPNAKNLEGVDHFDTPDPYSFVI"
     gene            complement(498..1658)
                     /locus_tag="VGA15_13590"
     CDS             complement(498..1658)
                     /locus_tag="VGA15_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9588762.1"
                     /translation="MYGHMRAAGLPVGYPQFFARGEGCYLWDVDGRKYLDFMCSWGPV
                     VLGHRHPHVDDAARRQMEQGDCLNGPTERMVELAERLVGLVTHADWAMFSKNGTDATT
                     TCVTLARAATGKRKILLASGAYHGAVPWCSPYPHGVTAEDRAHAIYFKYNDIDSLEAA
                     AGQAGSDLAGILVSAFRHDYGQHQELPDPAFAARARALADANEAALILDDVRAGFRLT
                     LHGSWDQIGVQPDLSAWSKAIANGYALAAVTGNERFRQAASQLYATGSFWCGATSMAA
                     ALATIDVLQAENGPAHMRQMGQRLRDGIAAQAVRHGVAVRQSGPPQMPTILFEGDADY
                     AKGSRFVLEALKRGVYLHPKHNMFLSLAHTVKDIDAALAVTDEAFGVIAKLQ"
BASE COUNT          353 a          568 c          531 g          339 t
ORIGIN      
        1 aatgacaaaa ctgtagggat cgggcgtatc gaaatgatcg accccttcga gattttttgc
       61 gttcggactg acgcgctgat agcgctcgaa cgatgccttg acgtcggccg aggtcatctc
      121 ctggccgtta tggaatttca cgcctttgcg cagttcgaac gagtaggtct tgaagtcctc
      181 cgaagccgtc gctttcgatg ccagcatggt gcgcgtcgcg ttgtttgcgt ccagcgtgac
      241 cagtccttcg aacacattgt tgatgacttc gagctcgacc gaactggagg cgacataggg
      301 atcgagtgtt ccgggtccgg agacgctggc atagaccagc gtgcctccct tctttggctg
      361 ctgcgcacaa ctgacgtcgc tcgcggcaag gtgcaccgca agcaccagga gaatcccgga
      421 aaggcatcgc gcaacacatc gcttcattcg cttcaatccc tcgagttaca tcgcaaggcc
      481 tcgatcaccg ctagcagcta ttgcaatttc gctataaccc cgaacgcctc atccgtgacc
      541 gctagggccg catcgatatc cttcacagtg tgggcgagcg aaaggaacat attgtgcttg
      601 ggatgcaggt agacgccgcg cttgagcgct tccagcacga agcgggagcc tttggcataa
      661 tcggcatcgc cttcgaacag gatcgtcggc atttgcggcg gaccgctttg ccggacagcg
      721 acgccgtgac ggaccgcctg agccgcgatg ccgtcgcgca atcgctgacc catctgccgc
      781 atgtgcgccg ggccgttctc ggcctgcagc acgtcgatcg tcgccagcgc agccgccatc
      841 gacgtcgcac cacaccagaa tgaaccggtc gcataaagct gtgatgctgc ctgccggaag
      901 cgctcgttgc cggtaaccgc tgcgagcgcg taaccgttgg cgatcgcttt gctccatgca
      961 ctgagatccg gctgcacgcc aatctggtcc cagctgccat gtaatgtcag gcgaaagccc
     1021 gccctgacgt cgtcgaggat cagcgcggct tcgttggcat cggcgagcgc gcgagcgcgc
     1081 gcggcgaagg cgggatcagg caattcctga tgctggccgt aatcgtgacg aaacgccgac
     1141 accaggatgc cggccagatc gctgccggcc tgccccgccg cggcttccag gctgtcgatg
     1201 tcattatact tgaaatagat cgcatgggcg cgatcttcgg ccgtcacgcc atgcggatag
     1261 ggcgagcacc acggcacggc gccgtgataa gcgcctgaag ccagcagaat cttgcgcttg
     1321 ccggtcgcgg cgcgcgccag cgtgacgcag gtcgtggtgg cgtcggtgcc gttcttggaa
     1381 aacatcgccc aatcggcatg cgtcaccaat ccgaccagtc gctccgccag ttcgaccatg
     1441 cgctcggtcg gaccattcag gcagtcgccc tgctccatct gccggcgcgc ggcgtcatcg
     1501 acgtggggat ggcgatgccc gaggacgacc ggaccccagc tgcacatgaa atcgagatat
     1561 ttgcggccat cgacgtccca gagataacag ccctcgccgc gcgcgaaaaa ctggggatag
     1621 cccacgggca agccggctgc ccgcatatgt ccgtacatgc cgccgggcac gacttgaaga
     1681 gcgcgtcgac gcaggctttg gtccagaccc gcaatcctgc tttcagtcaa agccaacttc
     1741 tccggccaag tttcaaaaag gatatctcat gcggcaacag acacaaccgc t
//
LOCUS       DASWGM010000473         5166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2547844,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000473 DASWGM010000000
VERSION     DASWGM010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2547844"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2742
                     /locus_tag="VGA15_13595"
     CDS             <1..2742
                     /locus_tag="VGA15_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9588763.1"
                     /translation="YPPTIRKVNPADTPILVLGLTSDTLPLTTVDAFAENILLQKISQ
                     ISGVGLVGIGGQQKPAVRVQLDPQALAARGINLEDVRTALGQANVDLAKGTLNSPRQT
                     YTLNTNDQLLKADDYANLVIAYRNGAPVRIRDVGRAVSAPENDLIAGWYNQKRAVLLA
                     IQRQPGANVIETVNRIKAMMPVLQASIPPAVKVNIISDRTETIRASVSDVQFTLMLTV
                     ALVVMVIFIFLRNFWATVIPAVTVPLSLIGTFAVLYELGYSLDNLSLMALSIAVGFVV
                     DDAVVVIENIVRHMEEGASPFEASLKGAGEIGFTIVSITLSLIAVFIPLFLMGGYVGL
                     LFREFAITVSVALVLSLLISLTLTPMMCARLLKPESKQHGWLYRFFERGFDGLLNLYE
                     AGLKIVLRHRFITLMTMLGTIALTGYLYVLIPKGFFPQQDTGLIIGLSEAAQDISFQA
                     MAERQQALLDAVMRDPAVATVGAAVGAGGGNTTVNNGRVYIALKPQNQRGSMDQVLAR
                     LRTNLAKIQGITLYMQAAQDITIGGRLSKTQYQYTLDDADPGELNHWAGLFLDKIKAI
                     PGIADVATDQLNAGPMLDITIKREVASSYGILPYTIDNTLDDAFGQRIVSTMYTTLNQ
                     YHVVLEVDPKFQYGPEALNGIYVKSSSGQQVPLSTLVDSVVKVAPLVVNHQGQFPSVT
                     ISFNLLPGTAIGQAVSAIQKIEKDLGKPLSLQTSFQGNAQAFGASLSSTPILIAAALF
                     VIYLILGVLYESLVHPITIISTLPSAGLGALLLLMAVHYDLSVIAIVGIILLIGIVKK
                     NGIMLVDFAQHVEHEQGLTAEESIYRACIMRFRPILMTTMAALLGGVPMMLGSGVGSE
                     LRQPLGYAIVGGLALSQVLTLYTTPVVYIYLDRLQTWLFGEKKNKAASGHAEPAPAE"
     gene            2904..4151
                     /locus_tag="VGA15_13600"
     CDS             2904..4151
                     /locus_tag="VGA15_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate decarboxylase family bicupin"
                     /protein_id="HEX9588764.1"
                     /translation="MFSRRDMLAVSAAGAVMTATAARAASFGNPDLPPQGAINGKAPG
                     NLADPGPQSSAIGNQFPSAQFPPATDVGGMAMDWASFNNAPKRIQNGGWARQVTVEDF
                     AISKEISGVNMRLTAGGIRELHWHQAAEWAIMTYGSCRVTVLDALGRPYVADVEQGDL
                     WYFPAGQPHSLQGLGPDGCEFVICFDDGHANEFNTLLVSDWFAHTPPEVLAKNFGVPA
                     ETFAKIPLQNLWIFQGTLPGDLASDRAAVNKGENAPPHPFIFRLGSSAPTKQSKGGDV
                     RIADSSNFTVSTRIAAALVTVRPGGVREMHWHPNADEWQYYLKGKARMTVFNTGPNAL
                     TMDFNPGDIGYVKRNLGHYVENVGDTDMQFIGVFRAPQYEEISLSNWLTHTPPALVAQ
                     HLNIDEATIAKWPDNSPGVMPRS"
     gene            4244..4660
                     /locus_tag="VGA15_13605"
     CDS             4244..4660
                     /locus_tag="VGA15_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9588765.1"
                     /translation="MDARDYVTIGMTAEKTVTVTSEMTVGHFVAHMPQVYATPMMILH
                     MEMASGSAIASHLPEGFVSVGMDVKVRHLAATPVGRTVRAISRVLKIDPKGVVFEVEA
                     WDGDRKIGDGTHRRGIVNVLEFEKRFGVKQLAMSSD"
     gene            complement(4789..>5166)
                     /locus_tag="VGA15_13610"
     CDS             complement(4789..>5166)
                     /locus_tag="VGA15_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEX9588766.1"
                     /translation="QTVVLSALARNLHDAAAASPQAASWLAGIVVAVASNLVNNLPMG
                     LIATATSHAARVPAHVAGAILIGVDLGPNLSVTGSLATILWLIALRREGENIASLRFL
                     RLGLIVMPPALLLSLLALSAMFP"
BASE COUNT         1044 a         1623 c         1525 g          974 t
ORIGIN      
        1 tatccaccga cgatccggaa ggtcaacccg gctgacacgc cgatcctggt gctcggcttg
       61 acctccgaca ccctgccact gaccaccgtc gatgcctttg cggaaaatat tctgttgcaa
      121 aagatatcgc agatatccgg cgttggtctc gtgggtattg gcggtcagca gaagcctgcc
      181 gtccgcgtgc agctcgatcc gcaggccctg gcagcccgcg gcatcaacct cgaggacgtg
      241 cggacggcgc tcggccaagc caatgtcgat ctggcgaaag gcacgctcaa cagcccgcgc
      301 cagacctaca cgctcaacac caacgatcag ctgctgaagg ccgacgacta cgccaatctg
      361 gtcatcgcct atcgcaacgg agcgccagtc cgcatacgcg atgtcggccg cgccgtcagt
      421 gccccggaga acgacctgat agcgggctgg tacaaccaga aacgggcggt gctcctggcg
      481 atccagcgcc agcccggcgc caacgtcatc gagacggtga accggatcaa ggcgatgatg
      541 ccggtgctcc aggcctcgat cccgcccgcg gtcaaggtca acatcatctc cgaccgcacc
      601 gagaccatcc gcgcttcggt cagcgacgtg cagttcacgc tgatgctgac ggtcgcactg
      661 gtcgtaatgg tgatcttcat attcctgcgc aatttctggg ccacagttat tccggccgtc
      721 accgtaccgc tatcgctgat cggtaccttc gctgtactgt acgagctcgg ctacagtctc
      781 gataacctct cgctgatggc cttgtcgatc gcggtcggct tcgtggtcga cgatgccgtc
      841 gtcgtgatcg agaacatcgt gcggcacatg gaggaaggcg cctcaccgtt cgaagcgtcg
      901 ctgaagggag ccggcgagat cggcttcacc atcgtgtcga tcacgctatc gctgatcgct
      961 gtgttcattc cgctgttcct gatgggcggc tatgtcggac tgctgttccg cgaattcgca
     1021 atcacggtga gcgtggctct cgttctctct ctgctgattt ccctgacgct tacaccgatg
     1081 atgtgcgcgc gcctgctcaa gccggagagc aagcagcacg gatggctcta ccggtttttc
     1141 gaacgtggct tcgacggcct gctcaacctc tatgaggcgg gtctcaagat cgtgctgcgt
     1201 caccgcttca tcaccctgat gacgatgctc ggcaccatcg cgctgaccgg atatctctat
     1261 gtgctgatcc ccaagggctt ctttccccag caggacaccg gattgatcat cggcttgtcc
     1321 gaggcggcgc aggatatctc ctttcaggcc atggccgagc gccagcaagc gttgctcgac
     1381 gccgtgatgc gggaccctgc ggtcgccacg gtcggtgcgg cggtcggcgc cggcggaggc
     1441 aacaccacgg tcaataacgg ccgcgtttac attgcactca aaccccagaa ccagcgcggc
     1501 agcatggacc aggtgctcgc gcggctgcgg accaatctcg ccaagatcca ggggatcacg
     1561 ctctatatgc aagccgcgca ggacatcacc atcggcggcc gattgtccaa gacccagtat
     1621 cagtacacgc tggacgatgc cgatcccggc gagctcaatc actgggcagg gctgttcctc
     1681 gataagatca aggccatccc cggcatcgcc gacgtcgcca ccgatcaact caatgcgggg
     1741 ccgatgctcg acatcaccat caagcgcgaa gtcgcgtcga gctacggtat cttgccctac
     1801 acgatcgaca acacgctcga tgacgccttt gggcagcgca tcgtctcgac catgtacacg
     1861 actctgaacc agtaccatgt cgtcctggag gtcgacccca agttccagta tgggcccgaa
     1921 gcgctcaacg gcatctacgt caaatcgtcg agcggacagc aggtgccgtt atccacactc
     1981 gtcgactcgg tcgtgaaggt ggcgccgctc gtggtcaacc atcagggcca gttcccgtcg
     2041 gtgacgatct cgttcaacct cctgccgggc accgcgatcg gtcaggcagt aagcgccatc
     2101 cagaagatcg aaaaagacct cggcaagcca ctctcgcttc agaccagctt tcagggcaac
     2161 gcccaggcat tcggagcatc gctttcgagc acgccgatcc tcatagccgc cgcgctcttc
     2221 gtcatctacc tcattctcgg cgtattgtac gagagcctgg ttcacccaat caccatcatc
     2281 tcgacgctgc cgtccgccgg cctgggcgcg ctgttgctgc tgatggcggt tcactacgat
     2341 ctcagcgtga tcgcgatcgt cggcatcata ctgctgatcg gcatcgtcaa gaagaacggc
     2401 atcatgctgg tcgacttcgc ccagcacgtc gagcatgagc agggtctgac cgccgaagaa
     2461 tccatctatc gagcctgcat catgcggttt cggccgatcc tgatgaccac catggcggcg
     2521 ctgcttggcg gtgttccgat gatgctcggc agcggtgtcg gatcggagct tcgccagcca
     2581 ctcggctacg ccatcgtcgg cggcctcgct ctgtcgcagg ttctcaccct ctacacgacg
     2641 ccggttgtct atatctacct cgaccggctg cagacctggc tgttcgggga aaagaagaac
     2701 aaggccgcct ccggccacgc cgagcccgcg cccgccgaat gacggaggac aaaggatgcg
     2761 cagcgcgtat cgtgcggcaa gacggacttc ctcccagccc cgcccggtcc atgagacatt
     2821 ctgaatgcta atttagtgga tcacgacaat cgtgaaccta tctttcaagt acccacaatc
     2881 gattttgcag aggagtgata atcatgtttt cgagacggga catgctggca gtgtccgccg
     2941 cgggcgctgt tatgactgca accgcagcgc gagcggcctc ctttggtaac ccggacctgc
     3001 caccgcaagg cgctatcaac ggcaaggctc cgggaaatct cgccgatcct ggcccgcaaa
     3061 gctcggccat cggcaaccag tttccctcgg cccaatttcc gccggcaacc gatgttggcg
     3121 gcatggcaat ggactgggcc tcattcaaca atgcgccgaa gcgcatccag aacggcggct
     3181 gggcgcgcca ggtgacggta gaagactttg cgatttcgaa ggagatatca ggcgtcaaca
     3241 tgcggctgac cgccggcggc atccgggaac tgcactggca tcaggccgcc gagtgggcga
     3301 tcatgactta cggcagttgc cgcgtgacgg ttctcgacgc gttaggccgg ccgtacgtcg
     3361 cggacgtgga acagggcgat ctttggtatt ttccggcggg tcagccgcat tcgctgcagg
     3421 ggctcggccc ggacggctgt gaattcgtga tctgcttcga cgacggccac gccaacgaat
     3481 tcaatacgct actggtatcg gattggtttg ctcatacgcc gcccgaggtg cttgccaaga
     3541 atttcggcgt tccagcagaa acatttgcga agattccgct gcaaaatctc tggatcttcc
     3601 agggtaccct gcccggcgat ttggcgtcgg accgcgccgc ggtcaacaaa ggcgaaaatg
     3661 ctccacccca tccctttatc ttccggcttg gatcgtcagc tccgacaaag cagtccaaag
     3721 gcggcgacgt gcggattgcc gacagcagca acttcaccgt ctcaacgcga atcgcggccg
     3781 cgctggtaac ggttcgtccg ggcggggtgc gcgagatgca ctggcatccg aacgccgacg
     3841 aatggcagta ctacctcaag ggcaaggcac gcatgacggt tttcaatacc ggcccgaacg
     3901 cgctgacgat ggacttcaat cccggggaca tcggttacgt caagcgcaat ctgggacact
     3961 acgttgagaa tgtcggcgac accgacatgc agtttatcgg ggtgttcagg gcaccccagt
     4021 atgaagagat ttctctgtcc aactggctga cgcatacgcc gccggcattg gtggcccagc
     4081 acctgaatat agacgaggcg acgatcgcga aatggccgga caacagcccg ggcgtaatgc
     4141 ccagatcgta gatcgcggag ctggcgtcgg agcctttcag caacgccact ggcggcacca
     4201 gaaatggctg ctagcgcaca aagggatcac ggacgaggca gatatggatg cgcgcgatta
     4261 cgtcacgatc ggcatgacgg cggagaagac cgtgaccgtg acctccgaga tgacggtcgg
     4321 tcatttcgtg gcgcacatgc cacaagtcta cgccacgcca atgatgatcc tgcatatgga
     4381 aatggcatcg ggctccgcca tcgcttcgca tctgccggag ggttttgtga gcgtcggcat
     4441 ggacgtgaaa gtccgtcacc tcgcagcaac tccggtcggc cgcacggttc gcgccatttc
     4501 tcgcgtcctc aagattgatc cgaagggcgt ggtgttcgag gtcgaggcct gggacggcga
     4561 ccgcaagatc ggcgatggca ctcaccgccg cggcatcgtc aatgtgctgg agttcgagaa
     4621 gcggttcgga gtaaagcagc tcgcaatgag ttcggattga attcaatccg aactcacaac
     4681 tacgagcgca tccatttgcg ctcaaccccg cagcggtggc cgcgtcgtgc tctggccagc
     4741 gcagtacgca gtgcgcaatt cttcccagcc gaaggacgac tgtccgtttc atgggaacat
     4801 cgcggacaag gcaagcagcg acagcaggag cgcaggcggc atgacgatga ggccgagtct
     4861 gagaaaccga agagaggcaa tattctcgcc ctcccggcgc aatgcgatca gccacaggat
     4921 ggtggcgagc gagcctgtca cagaaagatt gggcccgaga tcaaccccga tcaggatcgc
     4981 accggccacg tgcgcgggta cccgcgcggc atgactggta gccgtcgcga tcagacccat
     5041 cggcagattg ttgacgaggt tggaggcaac agcgacgacg atgcctgcga gccacgacgc
     5101 cgcttgcggc gacgccgccg cagcgtcgtg caggttgcgg gccagcgctg acaacacaac
     5161 ggtttg
//
LOCUS       DASWGM010000474         4954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1991066,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000474 DASWGM010000000
VERSION     DASWGM010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991066"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VGA15_13615"
     CDS             <1..360
                     /locus_tag="VGA15_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9588767.1"
                     /translation="EGEPMMKAQCHCRECQYITGGSPNMFVLMSPDGFSYTKGTPKRF
                     ARSDLEKPVTREFCAECGTHLVTRPTAVPAVVLKVGTFDDPSLFGGPQMAIYTIDKQA
                     FHQIPDGMPSFERLPKR"
     gene            complement(400..1383)
                     /locus_tag="VGA15_13620"
     CDS             complement(400..1383)
                     /locus_tag="VGA15_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9588768.1"
                     /translation="MKVLITGSAGHLGEALVRTLTKMQCEVVSLDILESPFTTHVGSI
                     TDRSCVRHCMSGVQAVFHTATLHKPHIATHSRQDFVDTNVTGTLNLLEEAAAAGVASF
                     VYTSTTSIFGDALVPPPGAPATWVTEEVTPVPKNIYGVTKAAAEDLCQLFHRNQALPC
                     IVLRTSRFFPEEDDNRDVRDVYSDDNVKVNEYLHRRVEIEDVVTAHLLASEQAPAIGF
                     RKYIVSATTPFLPDDLPALRADAPRVVRLRVPEYEAEYERRGWKMFPSIGRVYVNGRA
                     QRELGWQPRYCFNDIIGRLKAENDLRSPLARVIGSKGYHAEAFPDGPYPVE"
     gene            1474..1755
                     /locus_tag="VGA15_13625"
     CDS             1474..1755
                     /locus_tag="VGA15_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588769.1"
                     /translation="MILKVGTFDDPSLFGGPQMAIYMFCPHPVQRAWYSGMDLATRRM
                     KCPSNSAKRADLADGQVMRYSRPLAGRFRWIGGLDQAWNPVFDRWLIVS"
     gene            1839..3053
                     /locus_tag="VGA15_13630"
     CDS             1839..3053
                     /locus_tag="VGA15_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9588770.1"
                     /translation="MAMTASSRAPQGSGNQESERSPFARLTELLAPYQPAKPLITLSL
                     GEPQHPVPAFVGPVLAKHVGDFGRYPIAKGIEPFRRAAANWLSTRFALPRGVDPETEL
                     LVLNGSREGLFFAALTAARYAGERKGRPAILIPNPFYPAYAAGARAAGCEAIYLPTTL
                     ANGFLPDLDGLDDGTLARTVAIFIASPANPQGAVASRAYFARLKRLADRFGFMILSDE
                     CYSEIYTREAPGSMLECAGPDFTNVVAFQSLSKRSNLPGMRVGFAAGDRKFLGMFHEL
                     RNVAAPQVPVPLQHVAVAAYSDEAHVEENRRLYRIKFDLADQILGNRYGYHRPAGGFC
                     VWLDVSARGGDEAATLKLYKDGGVRVVPGSYLARRQAGGSNPGAGYIRLALVQDSETT
                     AEALHRLVEILD"
     gene            3066..>4954
                     /locus_tag="VGA15_13635"
     CDS             3066..>4954
                     /locus_tag="VGA15_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX9588771.1"
                     /translation="MSMPAIERVIPLVGHLPASIREALARRLRELTGLALLSLSGVGA
                     AALMTWSVQDPSLSHATSRAIRNIVGYPGAIFADLLMQILGLGAIMLILPVAVRGWRM
                     LTHRTFDREALRIACWILCAVISAGFASCWPHGGAWPLPTGLGGVVGDALVRAPAVVF
                     GPPGIVYRLVLGVILFAAAAATLLIAGGMGSRPQEDELTPIEDDDAPFEEEEDDGSVS
                     LGWAFHAAMSAKARLWRLMTLAYSRLVASAPPQRASFERQEPNLGGRAAQSSVPLAAD
                     DSDEDEDEDDEIPAARAPRKKAAPKAASRKAWFELPPISVLTAPKASDRQPLSKSDLD
                     TNSRSLEGVLGDFGVRGEIVKAHPGPVVTLYELEPAPGIKSSRVIGLSDDIARSMSAL
                     SARVAVVPGRNAIGIELPNPHREKVYLRELLAAKDGNESTAKLPLCLGKNIGGESIIV
                     DLARMPHLLIAGTTGSGKSVAINTMILSLVYRLRPDQCRLIMVDPKMLELSVYDGIPH
                     LLTPVVTDPKKAVVALKWAVREMEERYKKMSKLGVRNIDGYNARLVEAKGKGEELART
                     VHTGFDKETGKAIYEEEKLDLEPLPYIVIIVDEMADLMMVAGKDIEGAVQRLAQMARA
                     AGLHV"
BASE COUNT          911 a         1531 c         1544 g          968 t
ORIGIN      
        1 gagggcgagc cgatgatgaa ggcgcaatgc cattgccgcg aatgccaata tatcaccggc
       61 ggctcgccca acatgtttgt cctgatgtcg ccggatggct tcagctatac caagggaacg
      121 cccaagcgat ttgcccgcag cgatcttgaa aaacccgtga cgcgggaatt ctgcgccgaa
      181 tgcggcacgc atctggtcac gcggccaacg gccgtgcctg ccgtcgtttt gaaagtcggc
      241 acattcgacg atcccagcct gtttggtggc ccgcagatgg cgatctacac catcgataag
      301 caggccttcc atcagatccc cgacggcatg ccgagcttcg aacgtctgcc gaaacgatga
      361 cacgtgtcca ggatagcgtc ggcggtgccc acaacgaacc tattcgaccg gataaggccc
      421 atcgggaaac gcctcggcat ggtatccttt tgatcctatc acccgcgcca gaggacttct
      481 caaatcgttc tctgctttca gacggccgat gatgtcattg aagcaatacc gcggctgcca
      541 gccaagttca cgttgcgcgc ggccgttgac ataaacccgt ccgatgcttg gaaacatttt
      601 ccagcctcgg cgttcgtatt cggcttcgta ttccggaaca cgcaaccgca cgacccgcgg
      661 tgcgtcggcc cgcagggcgg gcaggtcgtc aggcaggaag ggcgtggttg cgctgacgat
      721 gtatttgcga aagccgatcg ccggcgcttg ctcagaggcc aacaaatgcg cggtcacgac
      781 atcttcgatc tcgacgcgcc tgtggagata ttcattcacc ttgacgttat cgtctgagta
      841 gacatcccgc acatccctgt tgtcgtcttc ctctgggaaa aagcgcgacg ttctgagcac
      901 gatgcagggg agtgcctggt ttcgatggaa cagctggcag agatcctccg ctgcggcctt
      961 ggtcacacca taaatgttct tcggcaccgg cgtgacctcc tcggtcaccc acgtggcagg
     1021 ggctccaggt ggcggcacaa gggcatcgcc gaagatgctg gtcgtgcttg tgtagacaaa
     1081 agacgctacg cctgccgctg ccgcttcctc caacagattg agcgtaccgg taacgttggt
     1141 gtcgacgaag tcttgcctgg aatgggtagc gatgtgcggc ttgtgcaggg tggctgtatg
     1201 aaagaccgcc tgtacgccac tcatgcagtg cctcacgcaa gagcggtccg ttatcgagcc
     1261 cacgtgcgtg gtaaatggag actcaaggat atcgaggctg acgacctcgc attgcatttt
     1321 tgtcaacgtg cggaccaagg cctcgccaag gtgaccggca cttcctgtaa ttagaacctt
     1381 catgatcgcc cgacggcaat gttggaatag tgtcgaacgc taatagccga atgcggcgcg
     1441 catctaacca cgcggcggcc gagtttgccc gcggtgattc tgaaggtcgg cacgttcgac
     1501 gatcccagcc tgtttggtgg cccgcagatg gcgatctata tgttttgtcc acatcctgtc
     1561 caaagggcat ggtatagcgg tatggacttg gctacccggc gcatgaaatg cccatcaaat
     1621 tcagcgaaac gcgccgatct ggccgatggc caggtgatgc ggtattcacg tccgctcgca
     1681 ggccgcttcc gttggatcgg tggcctcgat caagcctgga acccggtgtt tgatcgatgg
     1741 ttaatcgtgt cttaacctcg ccgtatctat ggtgatttga tccgttttag gccggtctta
     1801 gagtgaccgg tcgccccaac agtgctggcg cttcacgtat ggcaatgacc gcttcatccc
     1861 gcgcgccgca gggctccggc aatcaggaaa gcgaacggtc gccattcgca aggctgactg
     1921 aacttttggc cccgtatcag ccagccaaac cattgattac actatcgtta ggcgagccgc
     1981 agcacccggt tccggccttt gtcggcccgg tgctcgccaa acacgtcggc gatttcggcc
     2041 gctatcccat cgccaagggc atcgagccgt tccggcgggc ggctgcgaac tggctttcca
     2101 cccggttcgc gctgccgcgg ggggtagatc cagagacgga gcttctggtg ctcaacggca
     2161 gccgcgaagg cctgtttttc gcagcgctca ccgccgcccg ctatgccggc gaacgcaagg
     2221 gcaggcctgc gatcctgata ccgaacccgt tttatcccgc ttacgcagcc ggcgcgcgcg
     2281 cggcgggctg cgaggcgatc tatctgccga ccacgctcgc caacggattt ctccccgacc
     2341 tcgatgggct cgatgacggc acgctggcga gaaccgtcgc gatcttcatc gcctcgccgg
     2401 caaatccgca aggcgcggtg gcctcgcgcg cctatttcgc acgcctgaag cgattagccg
     2461 atcgtttcgg cttcatgatt ctgagcgacg aatgctattc ggagatttac accagagaag
     2521 cgcccggcag catgctggaa tgcgcggggc ctgatttcac caatgtggtt gcgttccagt
     2581 cgctgtcgaa gcgatcgaac ctgccgggca tgcgggttgg ctttgcggcc ggcgaccgga
     2641 aatttctcgg catgtttcac gagctgcgca atgtcgccgc gccccaggtg ccggtgccgt
     2701 tgcaacatgt cgcggtcgcg gcctacagcg acgaggccca tgtcgaggag aaccggcggc
     2761 tctaccggat caaattcgat ctcgccgacc agatcctcgg caatcgctac ggctatcatc
     2821 gccccgccgg tggcttctgc gtctggctcg acgtgtcggc ccgcggcggc gacgaggcag
     2881 cgacgttaaa gctatacaag gacggcggcg tgcgcgtggt gcccggaagt tatctggcgc
     2941 gccggcaagc cggcggcagc aatcccggcg ccggctacat tcgtctggcg ctggtgcagg
     3001 acagtgaaac aacagccgag gcgttgcacc ggctggtcga aattctggat taatcgcagg
     3061 gccccatgag catgccagcg atcgaacgcg tcattcccct ggtcggccat ttgccggcgt
     3121 ctatccgcga ggcattggcg cggcgcttgc gcgaactcac cggccttgcc ctgttgtccc
     3181 tttccggggt tggggcggcg gccctgatga cctggtcggt acaggatccg agcctgagcc
     3241 acgcgacgtc gcgcgccatc cgcaacatcg tgggatatcc cggcgcgatc ttcgccgatc
     3301 tgttgatgca gattctcggc ctcggcgcga tcatgctgat cctcccggtc gcggtgcggg
     3361 gctggcgcat gctgacccat cgcaccttcg accgcgaagc gctgcggatc gcgtgctgga
     3421 tcctgtgcgc ggtgatatcg gcgggcttcg caagctgctg gccgcatggc ggggcttggc
     3481 cgctgccgac cgggctgggc ggcgtcgtcg gcgacgcgct ggtccgcgcg cccgccgtgg
     3541 tgttcggtcc gcccggtatc gtctaccgcc tcgtgctcgg ggtcatcctt ttcgccgccg
     3601 ccgccgcgac attgctgatc gctggcggca tgggttcacg cccgcaggaa gacgaattga
     3661 cgccgatcga ggatgacgac gcgccgttcg aggaagaaga ggacgacggc tcggtctcgc
     3721 tgggatgggc gtttcatgcg gcgatgagcg cgaaggcccg gctgtggcgg ctaatgaccc
     3781 tggcttacag ccggctggtc gcaagcgcgc cgccgcaacg cgcttcattc gaacgtcagg
     3841 aaccgaacct gggcggtcgc gcggctcagt cgtcggtccc actggccgca gacgattccg
     3901 acgaagatga ggatgaagac gatgaaattc ccgccgcccg cgcgccgcgc aagaaggccg
     3961 cgccgaaggc ggcatcgcgc aaagcctggt tcgagctgcc gccgatttcc gttctgaccg
     4021 cgccgaaggc ttcggaccgc cagccgctca gcaagtccga tctcgatacc aactcgcgct
     4081 cgctggaagg cgtgctgggc gatttcggcg tccgtggtga aatcgtcaag gctcatcccg
     4141 gtccggtggt gacgctgtac gaactcgagc ccgcgcccgg catcaaatcg tcgcgcgtca
     4201 tcgggctgtc cgatgacatc gcgcgctcga tgagcgcgct gtcggcgcgt gtcgccgtcg
     4261 tgcctggacg caacgccatc ggcatcgaat tgccgaaccc gcatcgcgaa aaggtctatc
     4321 tgcgcgaatt gctcgcagcc aaggatggca acgagtcgac tgcgaagctt ccgctttgtc
     4381 ttggcaagaa catcggcggc gaatccatca tcgtcgatct cgcgcgcatg ccgcatctgt
     4441 tgatcgccgg caccaccggc tccggcaaat cggtcgccat caacaccatg atcctcagtc
     4501 tggtttatcg gttgcggccg gatcaatgcc gcttgatcat ggtcgatccg aagatgctcg
     4561 agctctccgt ctatgacggc attccgcatt tgctgacgcc ggtcgtgacc gacccgaaga
     4621 aagcggtggt cgcgctgaag tgggccgtgc gcgagatgga agagcgctac aaaaagatgt
     4681 ccaagctcgg cgtgcgcaac atcgacggct acaatgcgcg tctggtggaa gcgaaaggaa
     4741 agggcgaaga gctggcacgc acggtgcata ccggtttcga caaggaaacc ggcaaggcga
     4801 tctacgaaga ggagaaactc gatctcgagc cgttgcccta cattgtcatc atcgtcgacg
     4861 aaatggccga cctgatgatg gtcgccggca aggatatcga aggcgcggtg caacggctgg
     4921 cgcagatggc gcgcgccgcc ggtctgcacg tgat
//
LOCUS       DASWGM010000475        11802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1846240,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000475 DASWGM010000000
VERSION     DASWGM010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846240"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VGA15_13640"
     CDS             <1..1246
                     /locus_tag="VGA15_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9588772.1"
                     /translation="ATGAAIGSDVVSGFPTIWAQEIKDIELRHVGVSYSVVKAIGDQA
                     AKDLGFKVTMQNLDTSAAINRFISQPDSVDIADLEGWQAKLAAKRSVIQGIEVKKIKE
                     FDNILPIFTKGEIDGHKIPRQGISPYEAMYIAKPNSTELNDGVTEWATFLPQVYNADS
                     IGYRPDLVGHEVTEWKDLIDPKFKGKAAILDVPAIGIMDAALCFESADLIKYGNKGNM
                     TKEEIDFTCNKLIELKKSGQFRATWTTFDQSVQLMAAGEVVIQSMWSPAVAAVRVKEI
                     PCVYAPVNVKNGKEGYRGWCNGMGLMKHLSGKKLDAAYEYLNWYLSGWQGGFVGRYGY
                     YSPVPSTAKKFLTAAEWDFWYDGKPAPSVINDPYGVPMEKAGTKRDGGSFVERVKNIS
                     CWNTLMDEAAYMNKRWNDFKVA"
     gene            1344..2204
                     /locus_tag="VGA15_13645"
     CDS             1344..2204
                     /locus_tag="VGA15_13645"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588773.1"
                     /translation="MLRHHLSSWLYVSPLMLVLVPFFLMPIVVVLAASFLETDGFGGL
                     LPSFTLANYLDVLTSAQTFHLYMATLKFTVLTWFFTLIIGFLVAYFLVFHVRNQLLAI
                     GLFLVCTVPFWTSNIIRMISWIPLLGKEGLVNSALLGTGLIHEPLEVLLYSGLAVVIA
                     YVHQLTIFMVVPIFNSMARIDKRIVEAALDAGASRLDVMRFIVVPMSKSGIALGSIFV
                     ISIVMGDFFVVKVMSGGSSASVVSAFYENVGVLQYPIAAASAVILTLVLIAVISLILR
                     AVDIRREITR"
     gene            2234..3094
                     /locus_tag="VGA15_13650"
     CDS             2234..3094
                     /locus_tag="VGA15_13650"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588774.1"
                     /translation="MAKSRRSVVARRGDSRPWTFYALATVFTLYVAALYGPMICIYIL
                     SFQDIRGGLVFPMKGHSLHWFVDLFTQVRTGDVKGSFDRSIKLAVLVTVITLLFSFMA
                     GMAFRRRFFGDTFVFYLMIGSLVAPGLVLGLGIGLLFQWVGINASWYTSALGAQLSWT
                     LPFGVLVMFAVMSRFDNVWEEAARDLGANRWQIIRLVVLPVLAPGLVAVALFGFTLSY
                     DEFARTLQTAGSLNTLPLEIWSMTLNVTSPSLYALGTVTTVVSFVIIGVSIGAIILIN
                     KRRGIRAGSH"
     gene            3106..4212
                     /locus_tag="VGA15_13655"
     CDS             3106..4212
                     /locus_tag="VGA15_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588775.1"
                     /translation="MRAGLGDIELAGLCKSYDGVTNVVDGVNLKIPDGAYCCFLGPSG
                     CGKTTILRMIAGHEDPSAGEIVIGGDNVVGLAPVERRTAMMFQSYALFPHLTVRDNIA
                     FALRVRGQSRAERYRAADMMMEKVRLTELAGRLPANLSGGQQQRVALARAAITEPRVL
                     LLDEPLSALDEQLRVQMRQELRRMQRELGITFVHVTHTQLEAIALADIVVVMEKGRIK
                     QSGAARDVYAHPRDRYVAEFLGGQNVLSGKVEKVSDDHIVISQPNRSGIQVGVRPSAS
                     VGIGDRIDLAVRRDDVELMRATSGGPGNGATALPSRVVAVEYQGSFVKVMLDTIPDEE
                     FVAYVPERMFFQDPFNVGDVLLATWSAGRARILA"
     gene            4234..4722
                     /locus_tag="VGA15_13660"
     CDS             4234..4722
                     /locus_tag="VGA15_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9588776.1"
                     /translation="MIVNFTLNGRSRTVDVPPATLVSELLRETLNLTGTHVGCDTSQC
                     GACVVLFNGEAVKSCTLLAPMLDGGTLMSIEGLAGAGGSNQLHPMQEAFHQNHGLQCG
                     FCTPGMVITAVAMAKKNPALTEAEVRHGLEGNICRCTGYQNIVASVLAGAAAMNASAI
                     GD"
     gene            4726..7113
                     /locus_tag="VGA15_13665"
     CDS             4726..7113
                     /locus_tag="VGA15_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9588777.1"
                     /translation="MSNIIGIGEAPRRKEDFRFLTGRGNYVADMKRPGSTFGVFIRSQ
                     HAHAVIRGIDKSAALALPGVEAVLTGDDVAADGLGSLPCGWGIHGTDGLPMKEPPFPM
                     LAQGKARFVGDMVAFVVADTLAQARAAAELVVIDYEVLPAVVGVLEAVRPGAPQLFDD
                     VPNNLCCDWELGDKAAVAVAFRKAAHVARLSLVNNRLIGNPMEPRAAIAEYNAGTGHY
                     TLWTTSQFPHVVRFLMAALVLNIPEQKMRVVAPDVGGGFGVKQFHYGEEAVITWAAKR
                     VGRPIKWVADRSEGFVSDRHGRDHLTEAELALDENGKFLALKVATLANMGAYLSTFGP
                     NIPTNLYAPLLGGVYTTPAIYCNVKVVFTNTVPVDAYRGAGRPEATFVVERIVDVAAA
                     EMGIDRVELRRRNIIPKEAYPYQTPVLVEYDSGDPMGCLEGALVAADVAGFAGRKAAS
                     ERDGKFRGLGYSTYIEACGLAPSRFAGRLGARGGLYESATVRVHPTGQVTVLIGTHNH
                     GQGHETTFAQIVSEKLGVSFDNVDIVFGDTDRVQFGMGTYGSRSLVVGGAALSKASDK
                     VVTKGKKIAAHLLEAGEHDIQFEAGVFSVAGTDRSKTFQEIAGAAYVPHNYPLEVLEP
                     GLEEQAYYDPVNFTYPGGCHIAEVEIDPDTGIVTLERYTAVDDVGTVINPMIVEGQLH
                     GGIVQGVGQALYESAVYDEVSGQLLSGSFMDYTMPRADHVPNMNVQTHSTRCTHTPMG
                     VKGCGEVGTIGSPAAVINAVVDALSHLGVQHVDMPATPNRIWRIIQNAAMPRAAE"
     gene            7125..7928
                     /locus_tag="VGA15_13670"
     CDS             7125..7928
                     /locus_tag="VGA15_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9588778.1"
                     /translation="MISFAFHSPRTVPEAAALLGSVSDSMPFAGGMTLLPTIKQRLAS
                     PAALVDLSQIPQLSAIAVEGHTLVIGAMARHVDVAESERVRHSIPLLATLAADIGDPA
                     VRNRGTIGGSIANNDPSADYPAAVMALAATIVTDRREIAADRFFTGLFETALEPGEVI
                     TAVRFPIPAAGGYAKFKSPASRYAVVGVCVVKTADQVRVAVTGAGPGVFRVAEMEAAL
                     ADSFEAGAVASIKVSAANLNSDIHADPEYRAHLITVMAKKAVTAAIATL"
     gene            7937..9394
                     /locus_tag="VGA15_13675"
     CDS             7937..9394
                     /locus_tag="VGA15_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX9588779.1"
                     /translation="MKPGSARANDAVETKELARLSAVELLNGYRAGLFTPRDVIEDVI
                     AALEQIDASCNVVVTSTYDQARAAADQATRAWASGGPQGRLVGVPVSIKDLVYVAGVP
                     AFGGAPMNKDLVPTVDAAAVTALKSAGAIITCKTTTCESGYKLTADSPVSGITRNPWN
                     RERTSGGSSGGAAAAVAAGCGPLAIGTDGVGSIRVPSSFCGVVGVKPTFGLVPRSPGF
                     SPPSWASLAHTGPIARTVADAALMLEVIAIHDVRDAASLKVAPRAFDTHPGELDGLRI
                     GASVDFGYAAVDPDVRGVFQSALASLASLGAEILPDCVTFAPDMLDRVLKPIAYTEQA
                     AAVAGRGADALASSDRDYREVIAQGRGYIGVDYVEAGYRRNSLRSSFLELFQTVDALV
                     TPTVAITAFAAGTLGVDRVAGVAVDRHLGWSPFSWPINLTGLPAATIPCGFDTDGLPI
                     GLQIVAPWLDEGLILRIAAAFEWAHPWAQFRPRMA"
     gene            complement(9485..10315)
                     /locus_tag="VGA15_13680"
                     /pseudo
     CDS             complement(9485..10315)
                     /locus_tag="VGA15_13680"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(10449..>11802)
                     /locus_tag="VGA15_13685"
     CDS             complement(10449..>11802)
                     /locus_tag="VGA15_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9588780.1"
                     /translation="AEDIRLVIEPKSRAVDPELLMESLFRLTELESRISLNLNVLVKG
                     RIPKVLGLAECLREWLDHLRDVLLRRSNYRKEQIEHRLEVLGGYLIAYLNIDKVIKII
                     RTEDEPKPALIKAFKLTEIQADAILNMRLRNLRKLEEFEIRTEDKHLRAELKGIKELL
                     GSETAQWSKVGEQVRKVRDLFGPKTPLGKRRTTFADAPEHDLAAIEEAFVEREPVTVV
                     VSDKGWVRTLKGHVADLSGLTFKTDDQLGSAFFAETTSKLLLFATNGRFYSLEVSKLP
                     GGRGHGEPIRMFIDMEQDASIVSLFVNKGGRKFMVASHEGQGFVVNEDDCVGNTRKGK
                     QVLNVEMPNEARALTTVTGDTVAVIGNNRKLLLFPIDQVPEMARGRGVRLQKYKDGGL
                     SDIATFDAKDGLTWKDSAGREFSATIKELADWRGNRADAGRLPPKGFPKSNKFGRGIE
                     "
BASE COUNT         2098 a         3676 c         3726 g         2302 t
ORIGIN      
        1 ggcgacgggc gccgcgatcg gctccgatgt cgtcagcggc tttccgacga tctgggcgca
       61 ggagatcaag gatatcgaat taagacacgt cggcgtgtcc tattcggtgg tcaaggcgat
      121 cggcgatcag gctgcaaagg acctcggctt caaggtcacc atgcagaacc tggatacctc
      181 ggcggccatc aaccggttca ttagccagcc ggattcggtt gacatcgccg acctcgaagg
      241 ctggcaggcc aagctcgccg cgaagcgctc ggtgattcag ggaatcgaag tcaagaagat
      301 caaggaattc gacaatatcc tgccgatctt caccaagggc gagatcgacg gccacaagat
      361 tccgcgtcag ggcatttcac cctatgaagc gatgtacatc gccaagccga actcgaccga
      421 gcttaacgac ggcgtcacgg aatgggcgac gttcctgccg caggtctaca acgcggactc
      481 gatcggctat cgtcccgacc tggtcggcca cgaggtcacc gagtggaaag acctgatcga
      541 tccgaaattc aagggcaagg cggcgatcct cgacgtacct gcgatcggca tcatggacgc
      601 cgctttgtgc ttcgaaagcg ctgacctgat caagtacggc aacaaaggca acatgaccaa
      661 ggaagagatc gacttcacct gcaacaagct gatcgaactg aagaagtcag gccagttccg
      721 cgcgacctgg accaccttcg accaatcggt tcagttgatg gccgccggcg aagttgtgat
      781 ccagtcgatg tggtcgccgg ccgtcgccgc ggttcgggtc aaggagatcc cttgcgtcta
      841 tgcacccgtc aacgtcaaga acggcaaaga aggatatcgc ggctggtgca acggcatggg
      901 cttgatgaag cacctgtccg gcaaaaagct cgacgccgcc tatgaatatc tcaactggta
      961 tctgtcgggc tggcagggcg gcttcgtcgg acgctacggc tactacagtc cggtgccgtc
     1021 gaccgccaag aaattcctca ccgcagcgga atgggatttt tggtacgacg gcaaaccggc
     1081 gccctcggtg atcaacgacc cctacggcgt gccgatggaa aaggccggca ccaagcggga
     1141 tggcggctcc tttgtcgagc gcgtcaagaa tatctcctgc tggaatacgc tgatggacga
     1201 ggccgcctac atgaacaagc gttggaacga cttcaaggtc gcctgatcga aaacttctgc
     1261 cggtgcggcg tgcgcatcgg cagacatcgt cccgcaattc agaaccggtt gaccccggac
     1321 aaaatcgagg caggccttaa ctgatgctgc ggcaccatct gagttcatgg ctgtacgtct
     1381 cgccgctgat gctcgtgctc gttccatttt tcctgatgcc gattgtcgtg gtgctcgccg
     1441 cgagcttcct cgagaccgac ggctttggtg gcctgctgcc gagctttacg ctggcgaact
     1501 acctcgacgt gctgacgtcg gcgcagacct ttcatcttta tatggcaacg ctgaaattca
     1561 cggtgctgac ctggttcttc accctgatta tcggctttct ggtcgcctat ttcctggtgt
     1621 tccatgtccg caaccagctg cttgcgatcg ggctgttcct ggtctgcacg gtgccgttct
     1681 ggacctcgaa catcatccgg atgatctcct ggattccgct actcggaaag gaagggctgg
     1741 tgaattcagc gctgcttggc accggcttga tccacgagcc gctcgaggtg ctgctgtatt
     1801 caggtctggc ggtggtgatc gcctatgtcc atcaactgac gatcttcatg gtggttccga
     1861 tcttcaattc gatggcgcgc atcgacaagc gcatcgtcga agcagcactc gacgccggtg
     1921 caagccggct cgacgtcatg cgcttcatcg tcgttccgat gtcgaagagc ggcatcgcgc
     1981 tggggtcgat tttcgtgatc tcgatcgtga tgggtgattt cttcgtggtc aaggtgatgt
     2041 ccggcggaag ttcggcttcc gtcgtcagcg cgttctatga aaacgtcggc gtgctgcaat
     2101 atccgatcgc cgcggcaagt gcggtgattc tcaccctggt gctgatcgca gtgatttcgc
     2161 tcatccttcg cgccgtcgat atccgccgcg agatcacccg atgacggttg cgatggaaag
     2221 gtctgacatc gcgatggcca aatcccggcg cagcgtcgtc gcgcgccgcg gcgacagccg
     2281 gccctggacg ttctatgcgc tggccaccgt tttcacgctc tacgtcgcag cactgtatgg
     2341 cccgatgatc tgcatctaca tcctgtcgtt ccaggatatt cgcggaggtc tggtgtttcc
     2401 gatgaagggc cactcgttgc attggttcgt cgatcttttc acccaggtgc gcaccggcga
     2461 cgtcaagggc tcgttcgatc ggtcgatcaa gcttgccgtt ctcgtcaccg tgatcacgct
     2521 gttgttctcg ttcatggccg gcatggcgtt tcgccgccgg ttcttcggcg acaccttcgt
     2581 gttctatctg atgatcggca gcctggtggc gccggggctg gtgctcggcc ttggcatcgg
     2641 cctgttgttc cagtgggtcg gcatcaatgc gagctggtac acctcggcgc tgggcgcgca
     2701 gctgtcgtgg acgcttccgt tcggcgtgct ggtgatgttc gcggtgatgt cgcgcttcga
     2761 caacgtatgg gaggaagcgg cgcgcgacct cggggccaac cgctggcaga tcatccggct
     2821 ggtggtgctt ccggtgctgg cgcccggtct ggtcgccgtc gcgctgttcg ggttcacgct
     2881 gtcttacgat gaattcgccc gcaccctgca gacggccggc tcgctcaata ccctgccgct
     2941 ggagatctgg agcatgacgc tcaacgtgac gtcgccctcc ctctacgcgc tgggcacggt
     3001 gaccacggtg gtttcattcg tcatcatcgg tgtcagcatc ggcgccatca tcctgatcaa
     3061 caagcgtcgc ggcatccgcg ccggcagcca ttgaggaatt cggccatgcg cgcgggtctc
     3121 ggcgacatcg aactggcggg tctctgcaag agctatgacg gcgtgaccaa tgtggtcgac
     3181 ggcgtcaacc tgaagatccc cgacggcgcc tattgctgct tcctggggcc atccggctgc
     3241 ggcaagacga cgattttgcg gatgatcgcg ggtcatgagg acccgtcggc cggcgaaata
     3301 gtcatcggcg gcgacaatgt cgtggggttg gcgccggtcg aacgccggac ggcgatgatg
     3361 ttccagtcct acgcgttgtt tccgcacctc accgtccgcg ataatatcgc tttcgcgctg
     3421 cgggtgcgtg gccaatccag ggccgaacgc taccgggcgg ccgacatgat gatggaaaag
     3481 gtgcggctga ccgagctggc gggtcgtctg ccggccaatc tgtccggcgg tcagcagcag
     3541 cgcgtggcgc tggcgcgcgc ggccatcacc gaaccgcgcg tcttgctgct ggatgaaccg
     3601 ctgtccgcgc tggacgaaca gcttcgcgtg cagatgcggc aggaactgcg gcgcatgcag
     3661 cgcgaactcg gcatcacctt cgtgcatgtt acccacaccc agctcgaggc gatcgcgctc
     3721 gccgatatcg tcgtcgtgat ggaaaagggc aggatcaagc agtccggcgc ggcgcgcgac
     3781 gtctatgccc acccgcgcga tcgctatgtc gcggaatttc tcggcgggca aaatgtgctg
     3841 tcaggaaagg tcgagaaggt cagcgacgac cacattgtca tctcgcagcc gaaccgaagc
     3901 ggaattcaag tcggcgtgcg gccgagcgcg agcgtcggga ttggcgatcg catcgatctg
     3961 gcggtccggc gcgacgacgt cgaactgatg cgggcaactt cggggggccc cggcaacggg
     4021 gcaacggcgc tgccgagccg ggtggtcgcg gtcgaatatc aggggtcgtt cgtcaaggtc
     4081 atgctcgaca ccattcccga cgaggaattc gtcgcctacg tcccggagcg aatgttcttc
     4141 caggatccgt tcaatgtcgg cgatgtcctg ctcgccacct ggtcggcggg ccgcgcccgc
     4201 atccttgcct gatgccagcc caacggaggt cccatgatcg tcaatttcac ccttaacggc
     4261 cgatcccgaa cggtggacgt cccaccggcg acgcttgttt cggagctgct tcgcgagacg
     4321 ctgaatctca ccggcactca cgtcggctgc gacaccagcc agtgcggcgc ctgcgtcgtg
     4381 ctgttcaacg gcgaggccgt gaagagctgc accctgctcg cgcccatgct ggacggcggc
     4441 acgctgatgt cgatcgaggg attggccggc gccggcggct ccaatcagtt gcatccgatg
     4501 caggaagcgt ttcaccagaa tcacggcctg caatgcgggt tctgcacgcc cggcatggtg
     4561 ataacagcgg tggcaatggc caagaaaaac ccggcgctga ccgaagccga ggttcgtcat
     4621 ggcctggaag gcaacatctg ccggtgcacc ggctatcaga acatcgtggc atcggttctg
     4681 gccggtgcgg cggccatgaa cgcatccgcg atcggagact gacacatgtc caacatcatc
     4741 ggaattggcg aagcaccgcg ccgcaaggaa gacttccgct ttctgaccgg tcgcggcaac
     4801 tacgtggccg acatgaagcg ccccggttcg acgttcggcg tgttcatccg ttcccagcat
     4861 gcccatgcgg tgatccgggg catcgacaaa tccgcagccc ttgcccttcc gggggtcgag
     4921 gccgtgctga cgggcgacga cgtggcggcc gacggtctcg gatccctgcc ctgcggctgg
     4981 ggcattcacg ggaccgatgg gctgccgatg aaagagccgc cgttcccgat gctggcacag
     5041 ggcaaggcgc gctttgtcgg cgacatggtg gcattcgtcg ttgccgacac gctcgcgcag
     5101 gcccgcgccg ccgcggaact tgtcgtcatc gattacgaag tcttgccggc cgtggtcggc
     5161 gtgctcgaag ccgtgcgtcc aggcgctccc cagctgttcg acgacgtgcc gaacaacctt
     5221 tgctgcgact gggagctcgg cgacaaggcc gccgtcgcgg tcgcattccg caaggcggcc
     5281 catgtcgccc ggctaagtct cgtcaacaat cgactgatcg gaaatccgat ggagccacgc
     5341 gcggcgatcg ccgaatacaa cgcgggaacc gggcactaca cgctgtggac gacaagccag
     5401 ttcccgcatg ttgtgcggtt tctgatggcc gccctggttc tcaacatacc cgaacagaag
     5461 atgcgcgtgg tcgcgccgga tgtcggcggc ggcttcggcg tcaagcaatt ccactatggc
     5521 gaagaggcgg tcattacctg ggcggccaag cgggtcggac ggccgatcaa gtgggtcgcg
     5581 gaccgttcgg agggctttgt gtccgatcgc catggccggg atcacctgac cgaagccgaa
     5641 ctggcgctcg acgagaacgg aaaattcctc gccctgaaag tcgccacatt ggccaatatg
     5701 ggcgcctacc tctcgacatt cgggccaaat attccgacca atctctatgc cccgctgctc
     5761 gggggcgtct acacgacgcc ggcgatctac tgcaacgtca aggtggtctt caccaacaca
     5821 gttccggtcg atgcctatcg cggcgcgggg cggccggaag caaccttcgt ggtcgaacgc
     5881 atcgtcgacg tcgccgccgc cgagatgggc atcgaccgcg tcgaattgcg ccggcgcaac
     5941 atcattccca aggaagccta tccctaccag accccggtgc tggtggaata tgattccggc
     6001 gacccgatgg gatgcctgga gggcgcgctg gttgccgccg atgtcgccgg ctttgccggg
     6061 cgcaaggcgg cgtcggaacg cgacggcaag ttccgtggac ttggctattc aacctatatc
     6121 gaggcctgcg gcctggcgcc gtcgcgcttt gccgggcgcc tcggcgcccg cggcggactt
     6181 tacgagagcg cgaccgtgcg ggtgcatccg accggacagg tgacggtcct gatcggcacc
     6241 cacaatcatg gccagggcca tgaaacgacc tttgcgcaga tcgtgtcgga gaaactcggg
     6301 gtgtcgttcg acaatgtcga tatcgtcttt ggcgataccg atcgggtgca gttcggtatg
     6361 gggacctacg gctcgcgctc gctggtggtc ggcggcgccg cgctgtccaa ggccagcgac
     6421 aaggtggtga cgaaaggcaa gaagattgcc gcgcacctgc tcgaggccgg cgaacacgat
     6481 attcagttcg aggccggggt tttctcggtt gccggcaccg accgcagcaa gacgttccag
     6541 gaaatcgccg gcgccgccta cgtcccgcac aattatccgc tggaagtgct ggagcccggc
     6601 ctcgaggagc aggcctacta cgatccggtc aacttcacct atcccggcgg ctgtcatatc
     6661 gccgaggtgg aaatcgatcc cgacaccggg atcgtcacgc tcgagcgata caccgccgtc
     6721 gatgacgtcg gcaccgtcat caatccgatg atcgtcgaag ggcagttgca tggggggatt
     6781 gttcagggcg tcggtcaggc gctgtatgag agcgcggttt atgacgaggt ctccgggcaa
     6841 ctgctgtccg gctcgttcat ggattacacc atgccgcgcg ccgaccatgt tccgaacatg
     6901 aacgtgcaga ctcattcgac gcgctgcacg cacactccga tgggcgtcaa gggatgcggc
     6961 gaggtcggga ccatcggatc gcccgcggcg gtgatcaatg cggtggtcga tgcgctttcg
     7021 cacctcggcg tccagcatgt cgatatgcct gctaccccca accgaatctg gcgcatcatc
     7081 cagaatgcgg cgatgccgcg cgccgcagag taggagcacg cgagatgatc tcttttgcgt
     7141 ttcacagccc gcggacggtg cccgaggcgg cagcactcct cggcagcgtt tccgacagca
     7201 tgccgttcgc cggcggcatg acgctgcttc cgacgatcaa gcagcggctg gcgtcgccgg
     7261 ccgcgctggt cgatctgtcg caaattccgc aactgtccgc gattgccgtc gaaggccata
     7321 cgttggtgat cggggcgatg gcgcgccacg tcgatgtcgc ggaatccgaa cgtgtacgcc
     7381 acagcattcc gctgctggct accctcgccg ctgatatcgg cgatcccgcg gtgcgtaatc
     7441 gaggcacgat cggtggctcg attgccaaca acgatccctc cgccgattat ccggccgcgg
     7501 tgatggcgct ggcggccacg atcgtaaccg accgccgtga aatagccgcc gaccgttttt
     7561 tcacgggact gttcgaaacc gccctggaac caggcgaggt tatcacggcc gttcgcttcc
     7621 ccattcccgc cgccggcggc tatgcgaaat tcaagtcacc cgcctcgcgc tacgcggtgg
     7681 tcggcgtctg cgtcgtcaag accgccgacc aggtccgcgt cgcggtgacc ggagcgggtc
     7741 ctggcgtttt ccgcgtggcc gaaatggagg ccgcgcttgc cgactcgttt gaggccggcg
     7801 cggtagcctc gatcaaggtc agtgcggcaa atctgaattc cgacattcac gccgaccccg
     7861 aatatcgcgc ccatctcatc accgtcatgg cgaagaaggc cgtgacggcc gccatcgcca
     7921 cattgtgagg catgcggtga agccgggaag cgctcgtgcc aacgatgcgg tcgaaaccaa
     7981 agaactggca cgactctccg ccgtcgagct gctcaatggc tatcgcgccg ggcttttcac
     8041 gccgcgagac gtcatcgagg acgtcatcgc ggcgctggaa cagatcgacg cctcctgcaa
     8101 cgtcgtcgtc acgtcgacct acgatcaggc gcgcgccgcc gccgaccaag cgacgcgagc
     8161 atgggcatcg ggcgggccac agggccggct ggtgggcgtg ccggtcagta tcaaggatct
     8221 cgtctacgtc gcgggcgtgc cggcgttcgg cggcgcgccc atgaacaagg acctggtgcc
     8281 gaccgtcgat gccgccgcgg tcacggcgtt gaaatcggca ggcgcgatca tcacctgcaa
     8341 gaccacaacg tgcgagtccg gatacaaact cacggccgat agccctgtct ccggcatcac
     8401 ccgcaatccc tggaataggg agcggaccag cggcgggtcg agcggaggcg ccgcggcggc
     8461 ggttgccgcc ggatgcggcc cgctcgcgat cggaactgac ggcgtcggtt cgattcgcgt
     8521 gccgtcctcg ttctgcggcg tcgttggcgt gaagccgacc ttcggtctgg tgccgaggtc
     8581 gcccggattt tcgccgccgt catgggcgtc gctggcgcat accgggccga tcgcgcgaac
     8641 cgtcgcggac gcagcactaa tgctggaggt gatcgccatt catgatgttc gcgatgccgc
     8701 gagtttgaag gtggcgccgc gtgccttcga cacccatcct ggagaactgg acggactgag
     8761 gatcggcgcc agtgtggact tcggctacgc cgcggtcgac cccgacgtgc gcggggtatt
     8821 ccaatcagcg ctggcttcgc tggcgtcgct tggcgccgag atattgccgg actgcgtcac
     8881 attcgcgccg gacatgctgg atcgcgtctt gaagccgatt gcctataccg agcaggcggc
     8941 ggcggtcgcg ggtcgcggcg ccgatgcgct cgcctcgtcc gacagggact accgcgaagt
     9001 catcgcgcaa ggccgcggct atatcggtgt cgactatgtc gaggcgggtt atcggcgcaa
     9061 cagcctgcgc tcgtcgttcc tggaattatt tcaaacggtc gatgcgctgg tgaccccgac
     9121 cgtcgccata acagcgttcg cggcgggaac gctgggtgtc gatcgcgttg ccggcgtcgc
     9181 cgtcgatcgc cacctcggct ggtcaccgtt ctcgtggccg ataaacctga ccggattgcc
     9241 ggctgcgacg attccctgcg ggttcgacac cgatggcctc ccgatcggac ttcagattgt
     9301 cgcgccgtgg ctcgacgagg gcttgatcct gcggatcgcc gccgcgtttg aatgggcgca
     9361 tccctgggct cagtttcgtc cccgcatggc ctgacaggct cacggtgtcg gcatcggttg
     9421 ccgtcagcgg caactccttt ccaagcaata gctcgtcgtc gagattgaga tagtgcgcga
     9481 gatactcgtg cagggctccg gcggccttgg aagttgcgcc cggcgatttg taaagcagaa
     9541 gatcgacctt tggcagcgcc ggcaagcctt ccttgatccc gatttcgcgc atgttgcgca
     9601 ccagcgcgct gcggccaagc acggtcaccg ccattcctgc gaatgccgcc gcctgcagcc
     9661 ctccgacgct atcgctgacg caggcgatac gccatctcag gcgtgacgct tccagccgtt
     9721 ccagggcgtg atcgcgatag atgttgcccg gcggcagcag cgcgagcggg attggggttt
     9781 ccttgtgcgc cgtcgattgc tcccccgcca tccatatcaa ttgttcctgc cgcacgacct
     9841 ggccaccggt gaaatcattc atccgtgtca ccaaggcgat atcgacttcg ccgcgcttca
     9901 cacatccgac cagcggcgtc gacagcgagc agttcaattc gatctgcacc cgaggaaatg
     9961 tctttcgaaa aatcttgagg atcgaaggca gcatgaacgc ggcgtagaga tccggcgtgc
    10021 cgagcacgac atgtccctcg atatccgggg aggccagttg ggacagcagc tcgtcatgaa
    10081 atccgagtat cgacttggca taggaaagga cggtctcgcc gtcggcggtg agaaacagct
    10141 tgcggccttc atgggtaaag atcgccttcc cggtgagttc ttcgagccgc tgcaactgaa
    10201 gggtgatcgc cggctgagtc cgcccgagcc gtcgcgcggt ctcggtaatg cttccggtct
    10261 cgacgaccga agtcagcgag cggagcatcc tgatatccag gttgatgagg ttcattgaat
    10321 ggctctcaat cccgcgctgg gctgatccat gcaattttcg tgctggggat gcccggcgga
    10381 caaaagccac attcgtgcta acgaaagagg cggcggtgtg cgagggcagg aaccgcaatg
    10441 cttgcgccct actcgatccc gcgtccgaac ttgttcgact tcggaaaacc cttcggcggc
    10501 aaacggccgg cgtcggcgcg gttgccgcgc cagtccgcca gttccttgat cgtggcgctg
    10561 aattcgcggc cggcggaatc cttccaggtc aggccatcct tggcatcgaa tgtcgcgata
    10621 tccgacaagc cgccgtcctt gtatttctgc aggcgcacgc cgcggccgcg cgccatctcc
    10681 ggcacctgat cgatcggaaa cagcaaaagc ttgcggttat tgccgatgac ggcgacggtg
    10741 tcgccggtta ccgtcgtcaa ggcgcgggct tcgttcggca tctcgacgtt cagcacctgc
    10801 ttgcccttgc gggtgttgcc gacgcaatca tcctcgttga cgacaaaacc ctgcccttca
    10861 tggctcgcca ccatgaattt gcgtccgccc ttgttgacga acagcgacac gatcgatgcg
    10921 tcctgctcca tatcgatgaa catccggatc ggctcgccat ggccgcgccc gcccggcagt
    10981 tttgacactt cgagcgaata gaagcggccg ttggtagcga acaacaaaag cttcgacgtg
    11041 gtttcggcga agaaggcgga gccgagttgg tcgtcggtct tgaaggtcag ccccgagaga
    11101 tcggcgacgt ggcccttcag cgtccgcacc cagcccttgt cggaaaccac gacggtgacc
    11161 ggctcacgct ccacaaaggc ttcctcgatc gcggcaagat cgtgctccgg cgcgtcggca
    11221 aatgtggtgc ggcgcttgcc gagcggcgtc ttcggcccaa acaggtcgcg gaccttgcgg
    11281 acctgctcgc cgaccttgga ccattgcgcg gtctcggagc cgagcagctc cttgatgccc
    11341 ttcagttcgg cgcgaagatg cttgtcctcg gtgcggatct cgaattcctc gagcttgcgc
    11401 aaattgcgca ggcgcatgtt caggatggcg tcggcctgga tttccgtgag cttgaacgcc
    11461 ttgatcagcg ccggcttcgg ctcgtcctcg gtgcggatga tcttgatcac cttgtcgatg
    11521 ttcagatagg cgatcagata gccgccgagc acttccagcc gatgctcgat ctgctccttg
    11581 cgatagttgg agcggcgcag cagcacatcg cgcagatggt cgagccattc acgcaggcac
    11641 tcggcaaggc cgagcacctt ggggatgcgg cccttcacca atacgttgag gttcagcgaa
    11701 atccggcttt ccagctcggt cagccggaac agcgattcca tcagcaattc aggatcgacc
    11761 gcgcgggatt tcggctcgat cacgaggcgg atgtcttcgg cg
//
LOCUS       DASWGM010000476         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2129923,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000476 DASWGM010000000
VERSION     DASWGM010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129923"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..282
                     /locus_tag="VGA15_13690"
     CDS             64..282
                     /locus_tag="VGA15_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588781.1"
                     /translation="MAEGHVRVAWSRATDGQLVLRWTEAGGPRVNPPTRRGFGTSVTE
                     TMIRDRVKGEVRLDWQAEGLACEISVPM"
     gene            complement(520..1896)
                     /locus_tag="VGA15_13695"
     CDS             complement(520..1896)
                     /locus_tag="VGA15_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9588782.1"
                     /translation="MTDDTPLPFDLPAVHRKKLTVDFDGGTQSSDAGLLLLREAERKL
                     GVCRRLAAAMPDRRDPDRILHAMFEMVMERVSAIACGYKDAIDLDRLRHDPLMKVAVG
                     RCPETAAPLASQSTISRLENSPSKTDAAPLGEAMLDQFCATVKPGRVEIFDIDDTFCA
                     AHGGQQLAFWNAHHDERGFASMHIYHVASGAPVATILRPARTPKGTEVRTVVKHVTKR
                     LKQHWPNTRIVWRGDSHYGRVEAMEWIEENDGDYIFGLPGNSTLDALVAKTADNLRFH
                     HAKSRREKMRTHTSFTYQAGSWTRPRRVVARLECSLQPDAGETTSNGMRQEVDVRYVV
                     TSLKGRAEYLYEDVYCGRGQMENLIKLHKAQLASDRMSCHSATANQVRLALHTAAYWL
                     MLAVRDAIPQTEPLAKAEFATIRERLIKIGARVIEHAARIRIQLPTSCPEGWLFRTVA
                     LRLAPSSG"
     gene            complement(2199..2666)
                     /locus_tag="VGA15_13700"
     CDS             complement(2199..2666)
                     /locus_tag="VGA15_13700"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9588783.1"
                     /translation="MNTRAYSLTFISAGLMTLVGLSAATESKEYLKGDRPQQRGYSEA
                     VITEGGRMVWLAGQTATVDDNGRSLASDFDGQVRQLFKNLDRTLQKAGGKLSDMVQMT
                     VFITDVRYGDRLTQIRREVFGDNFPGSALITITALAVPDAKLEIQGYAVIGGK"
     gene            complement(2761..3468)
                     /locus_tag="VGA15_13705"
     CDS             complement(2761..3468)
                     /locus_tag="VGA15_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX9588784.1"
                     /translation="MLGAAATMLGIAPPAEATTPLSFLQENVMSEPIRPYEPLTSENA
                     GLVLIDHQVGLMTGVRDYSTGELKHNVVALAKAAMALNLPIIVATTARDSMWGPTFPE
                     LVEALPGVPIIDRSSVNAFDDPKVAQAITATGRKKLIFAGISLEVCAAFPAITAIGKG
                     LDAYVAVDASGTFSETKRQVGLLRMLKAGVILSDYATLMVEILKDNARPEAGAVYGAM
                     DMPWAKLVGQIAQAYGK"
BASE COUNT          643 a         1127 c         1122 g          705 t
ORIGIN      
        1 aggcgatagc tgtggcgctt cacgagctgg agaccaaatg cggcaaagta cggtgcccta
       61 tccgtggcgg aaggccacgt tcgtgtcgcg tggtcgcgcg cgacagacgg acagctcgtg
      121 ctccgctgga ccgaggcggg aggtccgcgc gtcaatccgc cgacccgcag gggatttggc
      181 accagtgtga cggagaccat gatacgagac cgcgtgaagg gcgaggtgcg gctcgactgg
      241 caggctgaag gtcttgcgtg cgaaatcagc gttcccatgt gagcttagcc gtccgacctt
      301 gatctctgac cgcgcgcggg ccgaggttcg cgcggtcact tcaccgacgc agcctttagc
      361 ccggatcatg caccacgcgg cgcgtttttg cacctccgac gcggaggcgc gcgccaccgc
      421 tccgaccggc tgatcggtgc gaacccactc actgtgcaac gcgtttgggg ttgatctgcc
      481 ttgctcggcg gcgcatctcg gggcacgaag ccccgccgct catccgctgg atggcgcgag
      541 gcgaagcgcg acggtgcgga ataaccatcc ctccgggcag ctggtcggca gttggatccg
      601 gatgcgcgcg gcgtgctcga ttacgcgcgc gccgatcttg atcagacgct cgcggatggt
      661 ggcgaactcc gcctttgcga gcggctctgt ctgcggaatg gcgtcgcgca cggcgagcat
      721 caaccagtat gcagcagtgt ggagcgcgag ccgcacctga ttggccgtcg cgctgtgaca
      781 cgacatgcgg tccgacgcca gctgcgcctt gtgcagcttg atcaggttct ccatctgccc
      841 gcgcccgcag tagacgtcct cataaagata ctctgcccgg cctttgagcg aggtgacgac
      901 gtagcggaca tcaacctcct ggcgcatgcc gtttgacgtc gtctctccgg catccggctg
      961 caacgaacac tccagtcgcg ctaccactct gcgcggacgt gtccaactgc cggcttggta
     1021 ggtgaagctc gtgtgggtgc gcatcttttc ccgacggctt ttggcgtgat ggaagcgcag
     1081 attgtcggca gtcttggcta ccagcgcgtc aagcgtcgag ttgccgggaa ggccaaaaat
     1141 gtagtcgcca tcgttctctt ctatccactc catcgcctcg acgcggccgt aatgactgtc
     1201 gccgcgccac acgatgcgag tgttcggcca atgctgcttc aggcgcttcg tcacgtgctt
     1261 gacgacggtc ctcacctccg tgcccttcgg ggtacgcgcc gggcgcagga tggtggcaac
     1321 cggcgcgccg ctcgcgacgt gatagatgtg catcgacgcg aagccgcgct cgtcgtgatg
     1381 cgcgttccag aacgccagct gctggccgcc atgcgccgca cagaacgtgt cgtcgatgtc
     1441 gaagatctca acccggcccg gcttcaccgt cgcgcagaac tgatcgagca tggcctcgcc
     1501 gagcggcgcc gcgtcggtct tgctcggcga attctccagc cggctgatgg tcgattgcga
     1561 cgccagcggc gctgcggtct ccggacagcg gccgaccgca accttcatca aaggatcgtg
     1621 ccgcagccgg tcaaggtcga tcgcatcctt gtatccgcac gcgatcgcag acacgcgctc
     1681 catcaccatc tcgaacattg catgcaggat acgatccgga tcgcggcgat ccggcatcgc
     1741 cgccgcaagc cgccggcaca caccaagctt gcgctcggcc tcccgcagca gcaacaagcc
     1801 ggcatcggag gactgcgtgc cgccgtcaaa atccactgtg agtttcttgc ggtgcactgc
     1861 tggcaggtcg aagggaagcg gcgtatcgtc ggtcatggcg gctgtggcgt tgggctgatt
     1921 gcacaaggca gttcgattgg acaccgattt gctacgtgaa tcggccactt cgcgctacag
     1981 tcgccgcaaa atttctacag ctgatgcata atccgggtta gccgggaggc cgcgcgggcg
     2041 cattatccgt ctcgcccatc acgcaaaacc gcgagaagga accgagttac ccactgcact
     2101 ggaccatcac cgggtaaaca ctgaccattg cgcgcggcgg actccggcgc gtttgctaga
     2161 aggtcaatgc tccgcccgcc gtcgcgcttt agaaattttc acttgccgcc aatcacggca
     2221 tatccttgga tctcaagctt ggcgtcagga accgcaagcg cagtaatggt gatgagagcg
     2281 ctgccgggga agttatcacc aaatacttct ctgcggattt gcgtgaggcg atccccgtaa
     2341 cgtacgtccg tgatgaatac ggtcatctgc accatgtcgc tgagcttgcc gcccgccttt
     2401 tgcagagtgc gatccaggtt cttaaaaagc tgccgtactt ggccgtcaaa gtcgctcgcc
     2461 agcgatctac cattgtcgtc cactgtcgcg gtctgtcctg caagccacac cattctgccg
     2521 ccttcggtga tcaccgcctc cgaatagccg cgctgctgtg gacggtctcc tttgaggtac
     2581 tccttcgatt ccgtcgcagc agacaaaccg actagagtca tcaaaccggc actgataaaa
     2641 gtcagtgagt atgctctcgt gttcatcttc tcctccttcc tgcctgtggt gggggcctcg
     2701 gccctactac gagggcaaca agataaccta gatcccggcc ggagccgacc ggcgcaccgc
     2761 ttacttgccg taggcctgcg cgatctgccc cacgagctta gcccagggca tgtccatggc
     2821 gccgtaaacg gcgccggcct ccggccgcgc attgtccttc aggatttcca ccatcaacgt
     2881 ggcgtagtcg gagaggatca cgccggcctt cagcatgcgc agaagaccga cttgtcgctt
     2941 ggtctcgctg aaggttccgg aggcgtcgac ggccacatag gcgtcgaggc ccttgccaat
     3001 cgcggtgatg gcggggaacg ccgcacagac ttcgagggag atgcccgcga agatcagctt
     3061 cttgcggccg gtcgccgtga tggcctgagc gacttttgga tcgtcgaacg cgttgactga
     3121 cgagcgatcg atgatcggaa cgccaggcag tgcttccacg agttcaggga aggtcggccc
     3181 ccacatgctg tcgcgcgccg tggtggcgac gatgatcggc aagttcagag ccatggccgc
     3241 cttggccagc gccactacgt tgtgcttgag ctctcctgtc gaataatcgc gaaccccggt
     3301 catgaggccg acctgatggt cgatgaggac gagaccggca ttttcagatg taagcggttc
     3361 ataagggcgg attggttcgg acatgacatt ctcctgcaga aaggaaaggg gagttgtggc
     3421 ttcggccggc ggtgcgatgc cgagcattgt cgcggcggcg ccgagcaccg ttgcttgaag
     3481 gaattgacga cgttccattt cggcctccgt ggagttccgg acgcgcctcg tgcgttcgag
     3541 ccgcggtcgt cggtcgcggc gcgtgacgag gtaattgctg gactatcggc cgagaag
//
LOCUS       DASWGM010000477         4581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2410748,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000477 DASWGM010000000
VERSION     DASWGM010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410748"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(430..957)
                     /locus_tag="VGA15_13710"
     CDS             complement(430..957)
                     /locus_tag="VGA15_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX9588785.1"
                     /translation="MMARTGWGGLLRMFRSLIIAFLLSFAAIANSNAQIVALGASSTA
                     GYGVGASSAYPAQLEAILRARGRAMSVSNAGISGDTTGGMLARLSSAVPEGTKIVILQ
                     IAGNDAMKGMSLTDAAANRAEIRRRLHARCIRTIEADGYVQSAVRSGLRQPDGIHMTV
                     EGHRKVAQQLAASIR"
     gene            complement(2181..2257)
                     /locus_tag="VGA15_13715"
     tRNA            complement(2181..2257)
                     /locus_tag="VGA15_13715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2221..2223),aa:Arg,seq:tct)
     gene            2563..>4581
                     /locus_tag="VGA15_13720"
     CDS             2563..>4581
                     /locus_tag="VGA15_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX9588786.1"
                     /translation="MIGLVRAATLCATLALGLVSAQAADKAFRRDDLADSAIKLEAQI
                     KSEAGPVAKSGVALRTDADAAFKRSDFRTGLQILGQIAATTPDDSANWLRLARTIFQI
                     RPANSSEQTFLLERASTAAYIAYQRAGNAGEEADALAVLGRAMSERKLWRPALDCLRL
                     SLDLREVADVRGQYEKMRDEYGFRLLDYTVDSDAASPRACFQFSEDLAKRTDFAPFLA
                     LAGSDKPALSAEGKQLCVDGLKHGERYNINLRAGLPSTVKETLPKSAEFNIYVRDRKP
                     FVRFTGKAYVLPRTGQRGIPLVSVNTPSVSVNVFRIGDRNLINTVIDSDFQRSLSKYE
                     LSSLGNERGVKVWSGELATATTLNQDVTTAFPVDQALGDLQPGVYVMTAAAKGPGSDD
                     DGSLATQWFIVSDLGLTAFSGNDGIHVFVNSLASTDAVSKADVRLVARNNEILATRKT
                     DESGHVLFEAGLARGEGGLSPAMLTVTSEKADYAFLSLKTDAFDLSDRGVSGRAVPSG
                     ADAFVYAERGVYRSNETVYLTALLRDGQGNAVTGGPLTLVIERPDGVEFRRAVLTDQG
                     AGGRSMAVALNSAVPTGTWRVRAFIDPKASSVGETTFMVEDYVPERIEFDLSAKDRQI
                     KAEAPVELKVDGHFLYGAPASGLQLEGDMLVAPASARPGFAGYQFGVAD"
BASE COUNT          870 a         1457 c         1400 g          854 t
ORIGIN      
        1 cttagcgcgg tcggacgatt gtcgggaaat acaggtgccg gaacattcaa tcgatccgac
       61 ggctgcgtcg gcagctggat cgcgatcaag cacgggccgt cgccaggact gcggcgatca
      121 agactgcggc gatgaagccg cgccgaataa ttgaggcaaa ctctaatgcc aagccttctc
      181 ctgctgatgt cccctttgga ttgacagcac acttcgcgtc ataacctgtg atattgttac
      241 aggtgaatga ggccgcaaac cagggcgttg aatgcgcgct tacacgacga aagagaaact
      301 taaaataact gccggggcag tgtccgcgct ggtcatcgtg gggtggatcg tcgtgctggc
      361 aattgtattt ctatccgtgc gatgaaaaaa tcagaccgcc aacggaggcg gcctctcaac
      421 caatgacgtt cagcggatcg acgcggccag ctgctgtgcg actttgcgat gaccttccac
      481 ggtcatatga attccgtcgg gctgcctcag cccgcttcga accgccgact gaacatagcc
      541 atcggcttcg atggtgcgaa tgcagcgggc gtgcagccgc ctgcgaatct cggcccggtt
      601 tgccgctgcg tcagtgaggc tcattccctt catggcatcg ttgccggcga tttgaagaat
      661 gacgattttg gttccttccg gcaccgcgct cgataatctc gccagcatgc cgccggtggt
      721 gtccccgctt atcccggcgt tgctgaccga catcgctcgt ccccttgcgc gcagaatggc
      781 ttccaattgg gccggatagg ccgacgacgc gccaacgccg tatccggcgg tgctgctcgc
      841 accgagcgcg acaatctggg cattggaatt tgcaatggcg gcaaagctaa gcaggaacgc
      901 gatgatcaag cttctgaaca tgcgcagcag tcctccccag ccggttcggg ccatcatccg
      961 cacgctcacc gtgtgggtca agattgcgcc gccggtcatc caccaaaggg gagacgtgtg
     1021 ccgctcggcc cgccggcggc ctcgtgcaat gcgacgatgg cctgtggtcg attggccggg
     1081 acagccaagg accactcgca aggactcaag aagggaaaga aagtggttgg aaggcgcggc
     1141 ctcccggctc cgtggccgga ttcgcaacta cttctcaacc atattggact attctgcttc
     1201 catggggacc cggcctgacg attccaagaa accagcagcg cgagcgcttc aacggcgcca
     1261 gacccttctg gccgcctggc tcgacgtcct ggacaagctc gatggtccga gccgccagcg
     1321 ccccgaaatt ccggccagat ccaccatccc atcgtcaaga cagccccgtt cggcgcgaac
     1381 ccggcggcgc ctgacagccc actccaaaga gggtcatgtg gccgccctta cagccaggcg
     1441 tcggaggacg gcgcctctct agatccgctc gtgttcggtg atcgttggat tgccgttcga
     1501 tccgacctca cgagagatac cggaatgttg ccgcgtgcgc ggctacctcg gcttggaaac
     1561 cgggttgctc cccgcattca atacgggttg gatcgtgagg cgcatggcta cgtcagtcga
     1621 gccgtcgaaa acctcaagga cctcgtcgca gaccagacaa cggaattcgc ccctggtccc
     1681 ctttttggaa acgagttcaa cccggcggta gccggcgtgg catttggggc aggcgacgtc
     1741 ggacttcttc atttttgcaa tatgtgcctg cgatgggacc gatcaagccg gttccgctgt
     1801 ctgtcggcct cacttgggcg ccggaaccgg cctgcgacca tgcggccggg ccgagcgggc
     1861 ggctctcgga taagtgattg aattcaaagc ccaccggccg agacggaacc ggagaggcga
     1921 ccatcaccgg ggcgacgcac atgctagcgg aaaagttcat tcttcttctg gaaacggtca
     1981 ttagccggac ctacccggac ggaagcccga ggatcgtaag cacatcgcgg cacgttccga
     2041 tccaactgcc gagcgagagc gggaagtaaa gtcggtggtc cggaaagttg gatgctggcg
     2101 gacttgggct gccgggcagc aacagcaact ggctcacttc cgaacccggc gaagcaaatt
     2161 gggaacggac ccagtccgaa tggcgatccc agcaggattc gaacctgcaa cccacggagt
     2221 agaaatccgt tactctatcc agttgagcta tgggaccgta acttcaggac acttcttcgg
     2281 gacacttggc cttcatagca ttgccaatat gaaaaatccg ctttccggcc aagcccctcc
     2341 gaacccttta tctgcgtggt gcgacaaagc ttgagcggtg ggccgttgcg tcaggtctac
     2401 ccgcaacgcc acgctccgcc agtcggccgc cggcgcggca agggctgttc gacgaatgtc
     2461 gcatgcgacc tttttgctaa cccattctaa tgccacgagt ccgtcacctt ttggcgcgtt
     2521 tgtcttcgac aggcggcgtt ggtccgccca gggagttcca tcatgatcgg tttggttcgc
     2581 gcggcaacgc tttgcgccac gctggcgctt ggcctggtct cggcgcaggc ggcggataag
     2641 gccttcaggc gcgacgacct ggcggattcc gcgatcaaat tggaggccca gatcaagagc
     2701 gaagccggcc cggtggcgaa gtcgggggta gccttgcgaa ccgacgccga cgccgccttc
     2761 aaacgcagcg atttccgcac cggtctgcag atcctggggc agatcgccgc cacaacacct
     2821 gacgacagcg ccaactggct gcggctcgcc cgcaccattt tccagattcg ccccgccaac
     2881 tctagcgaac agactttcct gctggagcgc gcctcgaccg cggcctacat cgcctatcag
     2941 cgcgccggca atgctggcga agaggccgat gcgctggccg tgctcggccg cgcgatgtcg
     3001 gagcgcaagc tgtggcgtcc ggcgctggat tgcttacggc tctcgctcga cctgcgcgag
     3061 gtcgccgacg tgcgcgggca atacgaaaaa atgcgcgacg agtatggttt tcggctcctg
     3121 gactataccg tcgattccga tgcagcctcg ccgcgcgcct gcttccagtt ctccgaggac
     3181 ctcgccaagc gcaccgactt cgcgccgttc ctggcgctgg cgggctcgga caagccggcg
     3241 ctgtcggcgg agggcaaaca gctctgcgtc gatggcctca agcacggcga gcgctacaac
     3301 atcaatctgc gtgccggatt gccgtccacc gtcaaggaaa ccctgccgaa gtcggctgaa
     3361 ttcaatatct atgtgcgcga ccgcaagccg ttcgtgcgct ttaccgggaa ggcctacgtg
     3421 ctgccgcgca ccggccagcg cggcattccc ttggtcagcg tcaacacgcc ctcggtatcg
     3481 gtcaatgtct ttcggatcgg cgatcgcaat ctgatcaata cggtgatcga cagcgatttt
     3541 cagcggagct taagcaagta tgaactctcc agcctgggca atgagcgcgg cgtaaaagtc
     3601 tggtcgggcg aacttgcgac cgcgaccacg ctcaatcagg acgtcaccac ggcgtttcca
     3661 gtcgaccagg cgctcggcga cctgcagccg ggcgtctatg tcatgaccgc cgcggccaag
     3721 ggccccggca gcgacgacga cggctcgctg gcgacgcagt ggttcatcgt ttccgatctg
     3781 ggcctgaccg cgttctccgg caatgacggc atccacgttt tcgtcaattc gctggcctcg
     3841 accgatgcgg tttccaaggc ggacgtgcgg ctggtggcgc gcaacaacga gattctggcc
     3901 acccgcaaga ccgacgagtc aggccacgtg ctgttcgagg ccgggctggc gcgcggcgag
     3961 ggcggtctgt cgccggcaat gctgacggtg accagcgaga aagccgacta cgcgttcctc
     4021 agcctgaaaa ccgacgcctt cgacctctcg gatcgcggcg tttccggccg ggccgtgcca
     4081 tcaggcgcgg atgcatttgt ctatgccgaa cgcggcgtct atcgctccaa cgaaaccgtg
     4141 tacctcacag cccttctgcg cgacggccag ggcaatgccg tgaccggcgg gccgctgacg
     4201 ctcgtgatcg agcgccccga cggggtcgaa ttccgccgcg ccgtgctgac agatcagggc
     4261 gcgggcggac gaagcatggc ggtcgcgctc aactccgccg tccccaccgg aacttggcgg
     4321 gtacgggcgt tcatcgatcc caaagcctcg tcggtgggcg aaaccacctt catggtcgag
     4381 gactacgtcc ccgaacgcat cgaattcgat ctctcagcca aggacaggca gatcaaagct
     4441 gaagctccgg tagaacttaa ggtcgacggc catttcctct atggcgcgcc ggcctccggt
     4501 ctgcagctcg aaggcgatat gctggtggcg ccggcttccg cgcgcccggg gttcgccggc
     4561 tatcagttcg gcgtcgccga c
//
LOCUS       DASWGM010000478         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2130026,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000478 DASWGM010000000
VERSION     DASWGM010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130026"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="VGA15_13725"
     CDS             <1..1887
                     /locus_tag="VGA15_13725"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX9588787.1"
                     /translation="AHGTTVATNALLQGEINSLGLIVNSGFRHILEIARQSVPEGYGN
                     SYFWVKPDRIVPLQFVREVTGRLDFRGHELRPLDEATVREAARYFRALGIRAIGICLL
                     HSYANDAHERRAAEIVAQEYPDATLSLSCVVLPEYREYERAVTTLVDAFVKPHMERYL
                     KRVHQELGVGLQDKPFLVMQSSGGVASADQVVRKPITTALSGPAAGVLGSAVVAEIAG
                     FADLVTLDAGGTSTDLCLIEGGKPHVTNGGSVGPFPVRIPMIDIETIGTGGGSIAWIS
                     REGHLKVGPRSAGAEPGPMCYPNGGVEPTITDANLVLGRIPPSLIGGGIVLNVEHARG
                     GIAALSAKLPGHMSVEQLASGIIEIANWNQANAIRQMTIQRGIDPREFALLSFGGAGP
                     AQSAHVMDLLGMKACIVPPNPGNLSAFGLLAVDWRTDHIVTKVMHEDAIDLNDVAARY
                     AALEREATATLQRDGIEASRIRLVREADIRYAGQSMEVRVTAPGGAIDKAFLSGLIGA
                     FNAAHLKTFGYNYAGRQKIELVNLCVSGFGVIERPGMPKLARRAGNPVPKSSRAVYFG
                     TRFQDTPVYDRTLLPSGGRVDGPAVVEEFGSTTVVFPGQYVEVDPHGILVIRPSKRSP
                     EAVR"
     gene            1884..3773
                     /locus_tag="VGA15_13730"
     CDS             1884..3773
                     /locus_tag="VGA15_13730"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX9588788.1"
                     /translation="MNIHHKSHPWPTAPLREAVSVDPIVLQIVEGTLNSIEAEIEYAI
                     ERTARSPMIREAHDFRVGLFDRYCRKLTGRSYSAMPNGVVRDFPPETMKPGDVFLMND
                     TYLTEGSIGHLPDLCSTVPVFHEGDVVAYIQAFGHHDDVGGRVPGSMPGTAATVFEEG
                     IAIPPVKIYSEGVRNDAVFTIVRRNTRVPEMLSADLDSEIQACLMGARRMAGLFERFG
                     KDQVEACFQAILDKCRDIYRNELLGKIADGEYAWEDYVEHDGITDPKLHKIALKMVKQ
                     GGKITLDFNGTDPQSTGPINWPADYADGAFLIKWIAPILRNLADTPERAAEIHVNEGV
                     CDVFEVIFPPKGTLITPEWPAATNARSFVLLRCLGLLAGVVAQAVDGRMPADQETIRY
                     TGFFGTDLDGKSFLSREVLGGGSGGRYYADGNDAIHIVPDSRNQPAEFTETRFPLLVE
                     KLALRTDSGGAGLRRGGQGYEKHYRALVDCRTIVTADRVRLGCYGLNGGKAGQPFCVT
                     IDAEGEARDLGGLVDGEAVLKGQVVRVVTTGGGGWGDPLQRELELVLRDVIEGRVSPA
                     SAEADYGVVFATASTADQPEIDEAATKARRADIEKARTAPPGMIDRGEGYEKMLRGEH
                     RPRMR"
BASE COUNT          739 a         1196 c         1302 g          724 t
ORIGIN      
        1 gcccacggca ccacggttgc caccaatgcg ctgttgcagg gcgagatcaa ttcgctgggc
       61 ctgatcgtca acagcggctt ccggcacatc cttgaaatcg cccggcagtc ggtgccggaa
      121 ggttatggca attcctattt ctgggtgaag cccgaccgca tcgtgccgtt gcaattcgtt
      181 cgcgaggtca ccggcaggct ggattttcgg gggcacgaat tgcgtccgct agatgaggcg
      241 acggttcgcg aagccgcgcg ttattttcgc gcgctaggta ttcgcgccat cggcatctgc
      301 ctgttgcatt cctatgccaa tgacgcacat gaacgccgcg ccgccgaaat cgtcgcgcaa
      361 gaatatccgg acgcgacgct ttcattgtcc tgcgtggtgt tgcctgaata tcgcgaatat
      421 gagcgcgcgg tgacgacgct ggtcgatgcc tttgtcaagc cgcacatgga acgctatctc
      481 aagcgtgtgc atcaggaact cggcgtcggc ctgcaggaca agccgttcct cgtgatgcag
      541 tcgagcggtg gtgtcgccag cgccgatcag gtggtacgca aaccgattac caccgcgctg
      601 tccggccccg cggcaggtgt gctcggcagt gcggtggtcg ccgaaatcgc cggctttgcc
      661 gatctcgtga ctctggatgc aggcggcacc tcgaccgatc tctgcctgat cgaaggcggc
      721 aagccgcatg tgacgaacgg cggatcggtc ggtccgtttc cggtgcggat tccgatgatc
      781 gacatcgaga ccatcggtac cggcggtggt tcgattgcct ggatcagccg ggaaggccat
      841 ctcaaggtag gtccgcgcag cgccggcgcc gagccggggc cgatgtgtta tccgaacggc
      901 ggcgttgagc cgaccattac cgatgccaat ctcgtgctgg gtcggattcc gccgtcgctg
      961 atcggcggcg gcattgtgct caatgtcgag cacgcgcgcg gcggcatcgc ggcgctttca
     1021 gcaaagctgc cgggccatat gagcgtcgag caactcgcca gcggcatcat cgaaatcgcg
     1081 aactggaatc aagccaacgc gatccggcag atgacgatcc agcgcggcat cgatccccgc
     1141 gaatttgcat tactttcatt cggcggcgcc ggaccggcgc agtcggcgca tgtgatggat
     1201 ctcttgggca tgaaggcctg catcgtgccg ccgaacccgg gcaacctctc ggcattcggc
     1261 ctgctcgcgg tcgactggcg caccgaccac atcgtcacca aggtgatgca cgaagacgcc
     1321 atcgacctta acgacgtcgc cgcgcgctat gccgcgctgg aacgcgaggc caccgcgaca
     1381 ctgcagcggg acggcatcga agcctcgcgc attcgcctgg tccgcgaagc cgacattcgc
     1441 tatgccgggc aatcgatgga ggtgcgggtg acggcgcccg gcggtgcgat agacaaggcg
     1501 tttctgtcgg gactgatcgg cgccttcaat gccgcgcatc tcaaaacctt cggctataac
     1561 tatgccggcc ggcagaagat cgagctggtc aatctctgcg tttccggctt tggcgtgatc
     1621 gagagaccgg gaatgccgaa gcttgctcgg cgcgcgggca atcccgttcc aaaatccagc
     1681 cgtgcggttt atttcggcac gcggtttcag gatacgccgg tctatgaccg tacattgctg
     1741 ccatcgggcg gtcgggtcga tggacccgcc gtcgtggagg agttcggctc gacgaccgtg
     1801 gtatttcccg gtcaatatgt cgaggtcgat ccgcatggta ttcttgtgat tcggccctcg
     1861 aagcgctcgc cggaggcggt ccgatgaaca tccaccacaa aagccatcct tggccgaccg
     1921 ccccgctgcg cgaagccgta tcggtcgatc cgatcgtgct gcagatcgtc gaaggcacgc
     1981 tgaattcgat cgaggccgag atcgaatatg ccatcgaacg caccgcgcgc tcgcccatga
     2041 tccgcgaagc gcatgatttc cgcgtcgggc tgttcgaccg ctattgccgc aagctcaccg
     2101 ggcgatccta ttcggcgatg cccaacggcg tcgtgcgcga ctttccaccg gagacgatga
     2161 agcccggcga tgtgttcttg atgaacgaca cctatctgac cgaaggcagc atcggccatc
     2221 tgccggacct ctgcagcacg gtgccggtat ttcatgaagg cgatgtcgtt gcctacatcc
     2281 aggccttcgg tcaccacgac gacgtcggtg gccgtgtgcc cggctcgatg ccgggcacgg
     2341 cggcgacggt attcgaagag ggcatcgcca ttccgccagt gaagatctac agcgaaggcg
     2401 tgcgcaatga cgccgtcttc accatcgtcc gccgcaacac ccgcgtgcct gaaatgttgt
     2461 cggcggatct cgatagcgaa attcaggcct gcctgatggg cgcgcggcgg atggccgggc
     2521 tgttcgaacg gttcggcaag gaccaggtcg aagcctgctt ccaggcgatc ctggacaaat
     2581 gccgcgacat ctaccgcaac gagctgctcg gcaagattgc cgacggtgaa tatgcctggg
     2641 aggattacgt cgagcacgac ggcatcaccg atccgaagct gcacaagatc gcgctgaaga
     2701 tggtgaagca gggcggcaag atcacgctcg acttcaacgg caccgatccg caatcgaccg
     2761 ggccgatcaa ctggccggcc gactatgccg atggcgcctt cctgatcaaa tggatcgcgc
     2821 cgatcctgcg caacctcgcc gatacgccgg aacgcgccgc ggaaattcac gtcaatgaag
     2881 gcgtctgcga cgtcttcgag gtgatttttc cgcccaaggg cacgctgatc acgccggaat
     2941 ggccggccgc gaccaacgcg cggtcgtttg tacttttgcg ctgcctcggc ttgctggcgg
     3001 gcgtggtcgc gcaggcggtg gacgggcgga tgccggcgga tcaggagacc atccgctaca
     3061 ccggattctt tggcaccgat ctcgacggaa aatcgttcct gtcgcgcgag gtgctcggcg
     3121 gcggctccgg cgggcgttat tatgccgacg gcaatgatgc gatccacatc gtgccggact
     3181 cgcgcaacca gccggcggaa ttcaccgaaa cccggtttcc gctgctggtg gagaagctgg
     3241 cgttgcgaac cgattccggt ggcgccgggc tgcgccgcgg cgggcagggc tacgaaaaac
     3301 actatcgcgc gctggtggat tgccgcacca tcgtgaccgc cgatcgcgtg cggctgggct
     3361 gttacggcct caatggcggc aaggccggtc agccgttctg cgtcaccatc gatgccgaag
     3421 gcgaagcgcg cgaccttggc ggcctggtcg atggtgaagc ggtgctcaaa ggccaggtcg
     3481 tgcgtgtggt gaccaccggc ggcggcggct ggggcgatcc gctgcagcgc gaactggagc
     3541 tcgtgttgcg cgacgtgatc gaagggcggg tttcgcccgc cagcgccgag gccgattatg
     3601 gcgtggtatt tgcgacagcg agcaccgcgg atcagccgga aatcgatgaa gcggcgacaa
     3661 aggcgcgccg ggccgatata gagaaggcgc gaaccgctcc gcctgggatg attgatcgcg
     3721 gcgagggcta tgaaaagatg ctgcgcggcg aacaccggcc gcgaatgcga tgaaactttg
     3781 gaagatgaaa cctgggcaag cgacagcgga gtggatggcg aatggcgatt gaagttgcga
     3841 aggtcgaatt gaagaccgtg caggacgcga gtggcctcga gggattgatc gcggccggca
     3901 agttcggcgc cgacgaagtg atcgcggtga tcggcaagac cgaaggcaat ggcggcgtca
     3961 a
//
LOCUS       DASWGM010000479        10914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1991731,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000479 DASWGM010000000
VERSION     DASWGM010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991731"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1213
                     /locus_tag="VGA15_13735"
     CDS             113..1213
                     /locus_tag="VGA15_13735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9588789.1"
                     /translation="MSVEFIGFITNNNSSETIVRTGPVLDRRYIETVAKAHETAGFDR
                     ALLAFHSTSPDCLQVGQHALGVTDRLKVLIAQRPGFTAPTLIARQLATVDQLSGGRVA
                     LHVITGGNATELKQDGNTIDDKDERYARTDEFLDVVRLEWTSEKPFSYNGKYYQVQNG
                     FSQVKPFRSEGIPIFVGGASDAAIEVAGKHADTFALWGESYAQVRDVTSRVQVAAATH
                     GRPSPRFSLSVRPILADTEEKAWAKAEEILARATALQDKTGYRKPADGHATAGARRLL
                     ALAEQGTRIDKRLWTEIAKLTGANSNTTALVGTPEQVAEVFGDYYDLGVSHFLIRGFD
                     PLIDAIEYGRELIPLTRKLIAERRLAKGVAAE"
     gene            1210..2151
                     /locus_tag="VGA15_13740"
     CDS             1210..2151
                     /locus_tag="VGA15_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588790.1"
                     /translation="MKRIVFAAGLLLGAMASATAQTTLRVGDQKGNSQAVMEAAGVLK
                     DVPYKIEWKEFPAAAPLLEALGAGAIETGLVGDAPFTFAAAANVPVKAIGAIRQSQEG
                     LAVLVPRESHIHSFEDLRGKKIATGRGSIGHQLILAALESRGWSAGDVQIVFLAPSDA
                     KVAYIQGSVDAWSTWEPYVSQEEVLFQSRRVITAQGLTPGIGFQVASANAIRDKRPEL
                     EDFLRRLTAARAWSLTNVSAYAETWGKLMNIPPAVPLNWLSRAKIRIAPIDDGVVADE
                     QKTIDLYFRAGLIKQQLNAADIVDRSFSHAIGKGAGL"
     gene            complement(2173..3690)
                     /locus_tag="VGA15_13745"
     CDS             complement(2173..3690)
                     /locus_tag="VGA15_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9588791.1"
                     /translation="MSLPKSAPIDGVLIGSTSELTAEAPAPVAAADVEAIDRISAVET
                     PAVPAAPDHPTKTMQTRSAILTGPVLPTLLRLALPTMTVLLAQTAVNVAEAYYVGFLG
                     TQALAGVALVFPVFMLMMMMSNGGLGSGVASAVARATGAGHKHDADALVLHALVLAVI
                     VGALFTIGAVWGGPVLYQALGGRDGSLNAALKYSNYLFAGAIPVWIVNFLAAALRGSG
                     NVRVPAMVTLVGALVLIPFSPILIFGFGPIPRLGIAGAGIAFALYYGAAMLVLLRYMT
                     TGRSGLTLKLGSLNGRLFKDILKVGLPTAFSTVLTNLTVILVTGAVGLFGTTALAAYG
                     IASRLDYVMIPILFGICTAVLTMVGVNMGAGQVARAKRIAWTSSFVGAGIAGSIGLIV
                     AIFPMLWLHLFSHDPQVLAAGSTYLRIVAPAYGALGLGFVIAFAAQGAGHVLWPFVGA
                     TSRLVLAAGCGWIAVSYLGGGMATLAAMVSTSLVAYAVICATAMFSGAVWRAQQG"
     gene            3809..4387
                     /locus_tag="VGA15_13750"
     CDS             3809..4387
                     /locus_tag="VGA15_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9588792.1"
                     /translation="MRYDKGHKDATRRRIIDVASRQFREHGIAAVGLAGIMTDAGLTN
                     GAFYAHFGSKEDLLREVLCNAGKRNEMWQAATESGAGLASTIRDYLSSGHRDNPGRGC
                     PTSALVAEIARHPKAARDAFTDKIAGIIELIAAHLDSGTQRERRRKAVAVYGLMVGTL
                     QLARAVNDKRLSEEILQSGAEQALALASQRGK"
     gene            complement(4427..5035)
                     /locus_tag="VGA15_13755"
     CDS             complement(4427..5035)
                     /locus_tag="VGA15_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9588793.1"
                     /translation="MVMGRPREFEVDKALDLALQVFWRKGYEGASMADLTEAMGITKP
                     SLYAAFGNKEELFRKALDRYVDGPGGYFQVALGMPTARAVVEHLLYEAADAVTDPNNP
                     PGCLAVQGALCCGDAAQSIKQELMLRRANGEEDLRKRFDRAVAEGDLPAGSDAADLAR
                     YISAILQGMAVQAAGGTSREQLRKIAEMALRTWPPTASISPK"
     gene            5225..6442
                     /locus_tag="VGA15_13760"
     CDS             5225..6442
                     /locus_tag="VGA15_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9588794.1"
                     /translation="MKNIIQNKRLIGAGLVLAVMATAGGATFLNGRIGAAQAETVAAA
                     PVALPVSVSVVTPRETVPWDEFSGRLEAVERVEVRSRVAGAIQEIHFREGALVKQGDL
                     LILIDPSLYAAEVARAEGQVAAAKARLVLTKSDFERGQQLTDSRTISQRDFDGRVNAY
                     SEADANLKAAEATLQTAKLNLGYTEVRAPVAGRVGKLEITVGNLIAAGPGTPVLTTLV
                     SVNPIYASFNADEQVVTRALRTLADQSTPSEIDRIPVQMGTGMSDGTPYKGHMQLIDN
                     QVDARSGTVRVRAVFDNADGRLMPGQFARLLMGQPKAEPTLLISERAVGTDQNKKFVM
                     VVDKDSKAEYREVALGVSIDGMRVVTGGLHAGERIVVKGLQRVRPGAVVAPQEIAMDS
                     VSADQTANKLAQR"
     gene            6586..9774
                     /locus_tag="VGA15_13765"
     CDS             6586..9774
                     /locus_tag="VGA15_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX9588795.1"
                     /translation="MNFSRFFIDRPIFAGVLSTLIFVAGLLSLPALPISEYPEVAPPT
                     VVVRAQYPGANPKVIAETVSTPIEEQINGVEGMLYMSSQATTDGLMTLNVTFRLGTDP
                     DKAQQLVQNRVAQAEPRLPEEVRTLGITTIKSAPDLTMVVHLLSPNGRYDMTYLRNYA
                     VLNVKDRLARIEGVGQVQLFGSGDYSMRVWLDPQKVAERSLSASDVVREIRAQNVQAA
                     AGVVGASPSEIGLDLQLSINAQGRLQTEEEFGDIIVKNGTNGEVTRLRDIARIELGAA
                     DYSLRSLLDNKSAVAVPIFQSPGSNAIQISDNVRKMMAELKQNMPDGMDYSIVYDPTQ
                     FVRASIEAVVHTLLEAVALVVLVVILFLQTWRASIIPLIAVPVSIIGTFAVMHVFGFS
                     INALTLFGLVLAIGIVVDDAIVVVENVERNIEQGLAPKQAAYQAMQEVTGPIIAIALV
                     LIAVFVPLAFITGLTGQFYKQFALTVAISTVISAFNSLTLSPALAALLLKSHDAPKDA
                     LTRGMDRLLGWFFQRFNRAFHRSSDAYSGGVKRVISRRAIVMVGYVALIGVTYGLFKA
                     VPGGFVPGQDKQYLVGFAQLPDGATLDRTEQVIRRMGDIALKLPGVESAVAFPGLSIN
                     GFTNSSNSGIVFVTLKPFEERKAASLSAAAIAKQLNGKFAGIQEAFIAMFPPPPVAGL
                     GTIGGFKMQIEDRAGLGYEALNEATKAFIAKASTAPELAGLFSSYQVNVPQLYADIDR
                     TKARQLSVPVTGVFDTMQIYLGSLYVNDFNKFGRTYSVRVQADAKFRARADDVGLLKV
                     RSDTGEMVPLSALLKIKESAGPERAMRYNGFLTADLNGGAAPGYSTGQAQAVVERIAA
                     ETFPKGIAFEWTDLTYQEIIAGNSSLVVFPVALLLVFLVLAAQYESLTLPLSIIMIVP
                     MGLLAAMLGVWLTKGDNNVFTQIGLIVLVGLSAKNAILIVEFARELEFAGRTPVRAAI
                     EASRLRLRPILMTSLAFIMGVVPLVISTGAGSEMRHAMGVAVFSGMIGVTAFGIFFTP
                     MFYVLLRALAGNRPLTQHGEASAEHIAASLID"
     gene            9972..>10914
                     /locus_tag="VGA15_13770"
     CDS             9972..>10914
                     /locus_tag="VGA15_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9588796.1"
                     /translation="MKRREFLKLSVATAAIAALSRQVRAQTTVTEIRIGYQKNGVLVI
                     ARQRATLEKHFASQGIAARWVEFSSGPPMLEAMNVGSIDYGAVGDSPPIFAQSAGAAI
                     VYAAGQPVTNGQGILVRRNSPIRSIMDLKGKRVGFTKGSSAHNIVVQTLEKAGLTYAD
                     ITPVYLTPPDAGPAFANGSIDAWSIWDPYFAIGEIKQQGRILVNASEITRTNSFYIAN
                     RDFAKNHGPILQQIIDVTSATAKWAEAHREEVAKSLSAVTGIPLDIQTVAANRSSFAV
                     GPITDDIVATQQGVADRFFKLGLIPKPVEVYDAVWKSV"
BASE COUNT         2156 a         3299 c         3415 g         2044 t
ORIGIN      
        1 ctgtgcgggc gcgttcatac gcctcaggac gacgcgacgc ggatcaacgc cgggcgcctg
       61 gcgctcggga tttgaagtgc atgcaagaat aaccggaacc cgggagctgc caatgtcagt
      121 ggaattcatc ggatttatca ccaacaacaa ttcatccgag accatcgttc ggaccggtcc
      181 tgttctcgat cgccgttaca tcgagaccgt agccaaagcg catgaaaccg ccgggttcga
      241 ccgggcgctg cttgcatttc attccacctc gccggactgc ctgcaggtcg gccagcatgc
      301 cctgggtgtg accgatcggc tcaaggtgct gatcgcgcag cggccgggtt ttactgcgcc
      361 gacgctgatt gctcgccagc ttgcgaccgt cgaccagctt tccggcggcc gggtggccct
      421 gcatgtcatc actggcggca atgccaccga gctgaagcag gatggcaaca ccatcgacga
      481 caaggacgag cgttacgccc gcaccgatga atttctcgac gtggttcggt tggaatggac
      541 cagcgaaaag ccgttttcgt acaatggcaa atattaccag gtgcaaaacg gcttttcgca
      601 agtcaaacca tttcgcagcg aaggcattcc catcttcgtc ggcggagcct ccgatgctgc
      661 gatcgaagtt gcgggtaagc atgccgacac ttttgcgctg tggggcgaat cctatgcgca
      721 ggtgcgcgat gtcacctcgc gggttcaggt tgccgcggcg acgcatggac ggcccagtcc
      781 gcgctttagt ctgtcggtcc ggccaatcct tgccgacacc gaggaaaagg cctgggcaaa
      841 agccgaggaa attctggcgc gggcaacagc cctgcaggac aagaccggtt accgcaaacc
      901 ggctgacggt catgcgaccg cgggcgcgcg gcggctgctg gcattggccg agcagggcac
      961 ccgcatcgac aaacgcctct ggaccgagat cgccaaactc accggcgcca acagtaacac
     1021 cacggcactg gtcggaacgc ccgagcaggt cgccgaggtt tttggcgact attacgatct
     1081 gggcgtcagc catttcctga ttcgcggctt cgaccccttg atcgacgcca tcgaatatgg
     1141 ccgcgaactg attcccttga cgcgcaagct gatcgccgag cgtcgcctcg ccaagggagt
     1201 tgcggcggaa tgaaacgcat tgtcttcgct gcggggctgc tgctgggcgc aatggcctcc
     1261 gcaaccgccc aaaccacctt gcgggtgggc gatcaaaagg gcaactcgca ggccgtcatg
     1321 gaagcggccg gcgtcctcaa ggatgtgccc tacaagatcg aatggaagga atttccggcg
     1381 gcagcacctt tgctggaagc gctgggcgcg ggcgccatcg aaaccgggct tgtcggcgac
     1441 gctccattca cctttgccgc ggccgccaac gtgccggtga aagcgatcgg cgccatccgg
     1501 cagagccagg aaggtcttgc ggtcctggtg ccgcgcgaat ctcatattca cagttttgag
     1561 gatctgagag gaaagaagat cgcgaccggc cggggctcga tcgggcacca gctcattctt
     1621 gcagcgctcg aatcaagggg atggtctgcg ggcgacgtgc agatcgtgtt tctggcgcca
     1681 tcggatgcca aggttgcgta cattcaggga tccgtcgatg catggtcgac atgggagccc
     1741 tatgtcagcc aggaagaagt gctgttccag tcgcggcgcg tgattacagc gcagggttta
     1801 actcccggaa tcggattcca ggtggcgtcg gccaatgcga tccgcgacaa gcggccggaa
     1861 ctggaagatt tccttcggcg gctcaccgcc gcccgcgcct ggtcgctgac caacgtcagt
     1921 gcttacgctg agacctgggg caagctgatg aacatcccgc ccgcggtgcc gctcaactgg
     1981 ctgagccggg cgaagatccg cattgcgccg atcgatgatg gtgtcgtggc cgacgaacaa
     2041 aagaccatcg acctgtattt tcgcgccggc ctgataaagc agcagctcaa tgccgccgat
     2101 atcgtcgatc gttcgttctc ccatgccata gggaagggcg ccggccttta gatcggctcg
     2161 cttctccggc gctcatcctt gctgggcccg ccagaccgct cccgaaaaca tcgcggttgc
     2221 gcagatcacg gcgtaggcaa cgagcgaggt gctcaccatg gctgcaagcg ttgccatgcc
     2281 gccaccaaga tagctgacgg cgatccagcc gcagccggcg gcgagaacga gccgcgaggt
     2341 tgccccgacg aagggccaaa gcacatgccc agcgccttgg gcggcgaagg caatcacgaa
     2401 tcccaagccc agggcaccat aggccggcgc gacgatacga agataggttg agcccgcggc
     2461 cagcacttgc ggatcgtggc tgaagagatg tagccaaagc attggaaaaa ttgcgacaat
     2521 caagccgatc gatccggcga tgccggctcc gacaaagctg ctggtccagg caattcgttt
     2581 ggcgcgggcg acctgaccgg cgcccatgtt gacgccgacc atggtcagca ccgcggtgca
     2641 gatgccgaat aggatcggga tcatgacgta gtcgagccgc gaggcgatgc cataggcggc
     2701 gagtgcggtg gtgccgaaaa gtccgaccgc acccgtcacc aggatcaccg tcagattggt
     2761 gaggaccgtg ctgaatgctg tcggcagacc gactttcaga atgtccttga acagccggcc
     2821 attgagagac ccaagtttta gggtcaggcc cgatcggccc gtggtcatgt agcgcagcag
     2881 caccagcatg gctgctccat aataaagagc gaacgcaatt ccggcgccgg cgattccgag
     2941 cctggggatc ggtccgaaac cgaaaatcag gatgggtgag aacgggatca gcaccagcgc
     3001 gccgaccaat gtcaccatcg ccggaaccct gacgttaccc gagccgcgca acgccgccgc
     3061 cagaaagttc acgatccaca ccggtatcgc gcccgcaaac agatagttgg aatattttag
     3121 tgcggcattc agcgatccat cccgaccacc aagcgcctgg tacaagaccg gaccgcccca
     3181 gaccgcgccg atcgtaaaga gcgcgccaac gatcacggca agaaccagag catgcaggac
     3241 aagcgcatcg gcatcgtgct tgtggccggc ccctgtggcg cgcgctaccg ccgaggcgac
     3301 accactgcct aatccgccgt tcgacatcat catcatcaac atgaaaaccg gaaacaccag
     3361 cgcgacaccg gcgagcgctt gcgttcccag aaagccgaca tagtaggctt cggcgacatt
     3421 caccgcggtc tgggcgagca ggaccgtcat cgtcggcagc gccaacctga gcagcgtcgg
     3481 cagaacaggc cctgtcaaaa ttgcggagcg cgtctgcatc gtcttggttg gatgatcggg
     3541 cgctgcgggg acggcgggag tttcaaccgc tgatattctg tcgatggctt cgacgtcggc
     3601 tgccgcgacc ggcgctggag cctcagccgt cagctctgac gtgctgccga tcaacacgcc
     3661 gtcgatcggg gcggattttg gcaggctcat tgtttttata tccgtcatca gttgcggaat
     3721 tcgccacttg cgaatgccgg gtattaatga gattattacc ataataccat attacgatcg
     3781 taatgcaatg agagaggagg aagcaaaaat gcgctacgac aagggacaca aggatgcgac
     3841 ccgccggcgc atcatcgacg ttgcttcccg gcaattccgg gaacacggca tcgccgcggt
     3901 cggacttgcc ggcatcatga ccgacgcggg cctgaccaat ggcgcgtttt acgcccattt
     3961 cggctccaag gaggatctgt tgcgggaagt tctttgcaat gccggcaagc gcaacgagat
     4021 gtggcaagct gccaccgaga gcggggccgg attggcgagc acgatccgcg attatctgtc
     4081 ttcaggccat cgcgacaacc ccggtcgagg ctgtccgacc tccgcactgg tcgccgaaat
     4141 tgcgcggcat cccaaggctg cacgtgacgc attcacggac aagatagccg gcatcatcga
     4201 gctgattgca gcccacttgg actcaggcac gcaacgcgag cgacggcgaa aggcggtcgc
     4261 ggtctacggg ttgatggtgg ggacgctgca gctcgcccgc gccgtcaacg acaagcggct
     4321 ttccgaggaa atcttgcaga gcggcgccga acaggccctt gcgcttgcca gccagcgtgg
     4381 caaatgagcc ggtggcttcc ttgaaaaggg tgccttccag ccgttgctat tttggcgaaa
     4441 tactcgccgt cggcggccat gttcgcaaag ccatttccgc gatcttgcgc aattgctcgc
     4501 ggctggtccc gcctgccgcc tggactgcca tgccttgcaa aatcgcggag atgtagcgcg
     4561 cgagatcggc cgcgtcggat cctgccggca aatcgccttc ggcgacggcg cggtcgaatc
     4621 gtttgcgcaa atcttcctca ccattggcac gccgcagcat caattcctgt ttgatggatt
     4681 gcgcggcatc gccgcagcac agcgcgccct gcaccgcaag acatcccggc ggattgttgg
     4741 ggtcggtgac ggcatcggcc gcttcgtaaa gcaggtgttc aacgacggcg cgtgcggtcg
     4801 gcattcccag cgctacctgg aaataaccac cgggtccgtc gacataccga tccagcgcct
     4861 tgcgaaacag ctcttccttg ttgccgaagg cggcataaag gctgggtttg gtgatcccca
     4921 tggcctcggt aaggtccgcc atcgacgcgc cttcgtagcc cttgcgccag aacacctgca
     4981 gcgcgaggtc gagcgcctta tcgacctcga attcacgcgg acgtcccatg accatgatgg
     5041 gtcctcctga gtttcctacc gctcgttaga taagtgactt gacaggggat gtcgatggcc
     5101 catctatacc gggcgttatg aatgtgtgcg atgcagcgta accgggtggg ttggcgtctt
     5161 cggccgatcc acccggttca agtcggggtt cgcaacctcg aacgtccttt ggaatggggt
     5221 ctccatgaaa aatatcatcc aaaacaaacg gttaattggg gcggggctgg tgctcgcggt
     5281 gatggctacg gccggcgggg cgaccttcct gaatggtcga atcggagctg cgcaggccga
     5341 gacggttgct gcggcgccgg tggcgctgcc ggtttcggtg tcggtcgtta cgccccgcga
     5401 gaccgtgccg tgggacgagt tttccggcag gctggaggcg gtcgagcggg tcgaagttcg
     5461 ctcccgcgtc gccggggcca ttcaggaaat tcatttccgt gaaggtgcgc tggtcaaaca
     5521 gggcgacctg ttgatcctga tcgacccgtc gctttacgcg gcggaggttg cgcgcgccga
     5581 aggccaggtg gccgcggcca aggcccgcct cgtcctgacc aagagcgatt tcgagcgtgg
     5641 ccagcagcta acggattcac gcacgatttc gcagcgggat tttgatggcc gcgtcaacgc
     5701 ctacagcgaa gccgacgcca atctgaaggc ggcggaagcc acccttcaga ccgcaaagct
     5761 caatctcggt tatacagaag tgcgcgcgcc ggtcgccggc cgagtgggaa agctcgaaat
     5821 caccgtgggc aacctgatcg ccgcgggacc cggcacgccg gtgctgacga cgctggtgtc
     5881 ggtcaatccg atctatgcca gcttcaacgc cgatgagcag gtggtgacgc gcgccttgcg
     5941 gacgctcgca gaccaatcga cgccttccga aatcgaccgc atcccggtgc agatgggcac
     6001 cggcatgtcg gacggcacgc cctacaaggg gcatatgcag ctgatcgata accaggtcga
     6061 tgcgcgcagt ggtacagtgc gggtgcgggc ggtgttcgac aacgccgacg gccgcctgat
     6121 gcctggccag tttgcaaggc tgttgatggg acaacccaaa gccgagccga cgctgttgat
     6181 cagcgagcgc gctgtcggca ccgaccagaa caagaagttt gtcatggtcg tcgacaagga
     6241 cagcaaggcc gaataccgcg aagtcgcgct cggcgtatcg atcgacggca tgcgtgtggt
     6301 gaccggcggt ctgcatgccg gcgagcggat cgtcgtcaag ggattgcagc gcgtgcggcc
     6361 gggagcggtc gttgcacccc aggagatcgc gatggattcg gtctctgccg accagaccgc
     6421 aaacaagctg gcacagcgct agggcaacct aactacgatc cttccgcgaa ctgcggaagg
     6481 atcagccgac acgatcgcga caacagcaat caatcagcgg ggcgatcgga atcgaccgcg
     6541 ccgggaggat tgcgctccgt aaccggatat cgacgagaag gtgccatgaa tttttccagg
     6601 tttttcatcg accggccgat cttcgcgggc gtgctgtcga cgctgatctt cgtggcggga
     6661 ctgctgtcgc ttccggcgct gccgatctcc gaatacccgg aagtggcgcc gccgaccgtg
     6721 gtggtgcggg cgcaatatcc gggggccaat cccaaggtca tcgccgagac ggtatcgacg
     6781 ccgatcgaag agcagatcaa cggggtcgag ggtatgctct acatgagcag ccaggctacc
     6841 accgacggat tgatgacgct caacgtcacg ttccgcctcg gcaccgatcc ggacaaggcc
     6901 cagcagctgg ttcagaaccg ggtggcgcag gccgaaccaa gattgcccga ggaagttcgc
     6961 acgctcggaa ttacgacgat caagagcgcg cctgacctta cgatggtggt gcatctgctg
     7021 tcgccgaacg gccgttatga catgacgtat ctgcgcaact acgccgtgct gaacgtcaag
     7081 gatcggctgg cgcgcattga aggcgtcggc caggtgcagt tgttcggctc cggcgactat
     7141 tccatgcgcg tctggctcga tccgcagaaa gtcgccgagc gcagcctttc ggcatccgac
     7201 gtcgtgcgcg agatccgggc gcagaacgtg caggcggcgg ccggcgtcgt cggcgcatcg
     7261 ccaagcgaga tcgggcttga tctgcaactc tccatcaatg cacaggggcg gctgcagacc
     7321 gaggaggaat tcggtgacat catcgtcaag aacggcacca acggcgaagt cacccggctg
     7381 cgcgacatcg cccgcatcga gcttggtgcc gcggactatt cgctgcgttc cctgctcgac
     7441 aacaaatcgg cggtcgcggt tccgatcttc cagtcaccgg gctcgaatgc gatccagatt
     7501 tcggacaacg tcagaaagat gatggccgag ctgaagcaga acatgcccga tggcatggat
     7561 tacagcatcg tctatgaccc gacgcagttc gtccgcgcat cgatcgaggc ggtcgtgcat
     7621 acgctgctgg aagcggtggc gctggtggtg ctggtcgtca tcctgttcct gcagacctgg
     7681 cgggcatcga tcattccgct gattgcggtg ccggtttcca tcatcggcac cttcgcggtg
     7741 atgcacgtct tcggcttctc catcaacgcg ctcacgctgt tcggactggt gctcgccatc
     7801 ggcatcgtcg tcgatgacgc catcgtcgtg gtcgagaacg tcgagcgcaa catcgagcag
     7861 ggcctggcgc cgaaacaagc ggcgtatcag gcgatgcagg aagtgaccgg cccgatcatc
     7921 gcgatcgcgc tggtgctgat cgcggtgttt gtgccgctgg cgttcatcac cgggctgacc
     7981 ggccagttct acaagcaatt cgcgctcacg gtggcgatct cgacggtcat ctccgcgttc
     8041 aattcattga cgctgtcgcc cgctctggcg gcgttgctgc tgaaatccca tgatgcgcca
     8101 aaagatgcct tgacccgcgg catggaccgg ttgctcggct ggttcttcca gcgtttcaac
     8161 cgggccttcc accgcagctc ggacgcctat agcggcggcg tcaagcgcgt gatctcgcgg
     8221 cgcgccatcg tgatggtcgg ctatgtcgct ctgatcggcg tcacctatgg tttgttcaag
     8281 gccgtgcccg gtggtttcgt gcccggtcag gacaagcagt atctggttgg ctttgcgcag
     8341 cttcccgatg gcgccacgct ggaccggacc gaacaggtga tccgccgcat gggcgatatc
     8401 gcgctgaaac tgccgggcgt cgaaagcgcg gtcgcgtttc ccggcctctc gatcaacggt
     8461 tttaccaaca gttcaaattc cggaatcgtg ttcgtgacgc taaagccgtt cgaggagcgc
     8521 aaggcggcga gcttgagcgc tgcggccatc gccaagcagc tcaacgggaa gttcgcgggc
     8581 atccaggaag ccttcatcgc gatgtttccg ccgcctccgg tggccggatt aggcaccatc
     8641 ggcgggttca agatgcagat cgaagatcgc gcaggccttg gctatgaggc gctcaacgaa
     8701 gccaccaagg cctttatcgc caaagcttcg acggcgccgg aactggccgg gttgttctca
     8761 agctatcagg tcaacgttcc acagctttat gccgatattg atcgcaccaa ggcacgtcag
     8821 ctcagcgtgc cagtgaccgg cgtgttcgat accatgcaga tctatctcgg ctcgctctac
     8881 gtcaacgatt tcaacaaatt cgggcgcacc tattccgtgc gggtgcaggc ggacgcgaaa
     8941 ttccgcgcac gagccgacga cgtcggactg ctgaaggtgc gttcggacac cggcgagatg
     9001 gtgccgctgt cggcgcttct gaagatcaag gaaagcgcgg gaccggagcg tgccatgcgc
     9061 tataacggct tcctcacggc cgacctcaat ggcggtgcgg cgcccggtta ttcgaccggg
     9121 caggcgcagg ccgtcgtgga gcggatcgcg gcggagacct ttccgaaagg cattgccttc
     9181 gaatggaccg acctgaccta tcaggaaatc atcgccggca actcctcgct ggtggtgttt
     9241 ccggtggcgc tgctgctggt gttcctggtg ctcgcagcgc agtacgagag cctcacattg
     9301 cctttgtcga tcatcatgat cgtgccgatg ggattgctgg ccgcgatgct gggcgtctgg
     9361 ctgaccaagg gcgacaacaa tgtcttcacg cagatcggtc tgatcgtgct ggtgggattg
     9421 tcggccaaga acgccatcct gatcgtggag ttcgcgcgcg aactggaatt tgccgggcgg
     9481 acgccggttc gggcggccat cgaagcgagc cggctgcggc tacggccgat cctgatgacg
     9541 tcgctcgcct tcatcatggg cgtggtaccg ctggtgatct ccaccggcgc gggatcggag
     9601 atgcgtcacg ccatgggcgt cgcggtgttc tccggcatga tcggggtaac ggcgttcggc
     9661 attttcttca ccccgatgtt ttacgtgctg ctgcgcgcgc tggccggcaa ccggccgctg
     9721 acccagcacg gtgaagcatc agcggaacat atcgctgcta gcttgatcga ttgagatccg
     9781 ttcacatttt atcgaggaaa aattgaacct gcctcggtga ggatcgttga aagaaaagca
     9841 gtgccgatta ccgatacgac agagcgcgca ttgctatttt tgcagagcac tcgacacttt
     9901 tccgaagcaa caccatcatt cctggaccga atgatcgcgc ggagttgcgg cattctggcc
     9961 aggagaccgc gatgaagcgt cgggaatttt tgaaactgtc cgttgcaacc gcggcaattg
    10021 cggcgctgtc gcggcaggtg cgcgcccaaa cgacggtcac cgaaatccgc atcggctatc
    10081 agaagaacgg cgtgctggtg atcgcgcggc aacgggccac gcttgagaaa catttcgcgt
    10141 cccaggggat cgcggcgagg tgggtcgagt tctcctcggg tccgccgatg ctggaggcga
    10201 tgaacgtcgg cagtatcgat tatggcgcgg tcggtgattc gccgccgatc ttcgcccagt
    10261 cggcgggagc ggccatcgtc tatgccgcgg gtcagccggt caccaacggg cagggcattc
    10321 tagttcggag aaattccccg atccgcagca tcatggattt gaagggcaag cgcgtcggct
    10381 tcaccaaggg ttccagcgct cacaacatcg tcgtgcaaac gctcgaaaaa gccggtctta
    10441 cctacgccga tatcacaccg gtctacctga cgccgccgga tgccggcccg gccttcgcca
    10501 atggcagcat cgatgcctgg tcgatctggg atccctattt cgcgatcggc gaaatcaaac
    10561 agcaggggcg tattctcgtc aacgcctccg agatcaccag gaccaattcg ttctacatcg
    10621 ccaaccgaga ttttgcgaag aaccatggtc cgatcctgca gcaaatcatc gacgtgacgt
    10681 ccgcgaccgc aaaatgggcc gaagcgcacc gcgaggaggt ggcgaagtca ttgagcgccg
    10741 tcaccggcat tcccctggat atccagacgg ttgccgccaa ccgctcgtcc tttgcggtcg
    10801 gcccgatcac cgacgatatc gttgccaccc agcaaggtgt ggccgatcgc ttcttcaagt
    10861 tgggtctcat tccaaagccg gtcgaggttt acgacgcggt ctggaagtcg gtgc
//
LOCUS       DASWGM010000480         8183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_728950,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000480 DASWGM010000000
VERSION     DASWGM010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728950"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2431)
                     /locus_tag="VGA15_13775"
     CDS             complement(<1..2431)
                     /locus_tag="VGA15_13775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9588797.1"
                     /translation="MTAAQIRSDLEVVARQTKAVRTYASTGGLELVPAIANEFGLNVG
                     MGAWIGNDEARNEREIRSSIQVARQNPNVTRLIVGNETIFRREHTADYMIRLLERVKR
                     ESPVPVTTAEQWPVWLEHPELANAVDCIFAHIIPYWEGFSEKIAVDQALLIYNKLHAA
                     FPGKPIVIGEFGWPSGGHNIKNAMPGRGEQAKVLRDFVTRADAAGIDYNIVEAIDQPG
                     KVFEGSVGPYWGIFSASLQPKFAWVGAVGNADRWRTAAVAVLGGILLSLPVFALSGGT
                     FGQVAVLAISAHAAGAWCATVLAYWHSHYFTPGEAVAFGPGLALLALLIAMALARLEE
                     LAAVALGPEPRRMLPASVAAREGFAPKVSIHIAARREPADMLKLTLDAVARLRYSNYE
                     CVVVINNTPEPSLWRPIEEYCRALGERFKFIYVEKLEGFKAGALNLALANTAADAEII
                     GVLDADYVVHPDWLKDLIPTFYDSSVALVQAPQDHRDGDRSIVHAAMNSEYAGFFDVG
                     MVQRNETNAIIMHGTMCLIRRAALEAAGRWSSDTICEDTDLGLTILELGWRAHYTNRR
                     YGWGLLPESYEAFKKQRYRWACGGAQILKKHWRRFLPGASRLDHDQKREFVIGWLRWL
                     GGETIAVAAAMLNLVWVPLVIFFGLAIPDTILMLPTIVAFMLSLLHFSIAYRLRVDSP
                     LRAAAGALFAAMSVQWTVANAIAHAAIGRDQKRFHRTAKGGRGDRSVGFAAFWEVTMG
                     GLLVAGATAVWITNYDQIRANYFFAGALLVQSLPFLSAAMLAALESSRLNKRRYWRRL
                     EIRISRLLGVAR"
     gene            complement(3248..4585)
                     /locus_tag="VGA15_13780"
     CDS             complement(3248..4585)
                     /locus_tag="VGA15_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX9588798.1"
                     /translation="MLSVARRSAIPGRDLRLDFFRGLALWLVFLDHIPSNVVSWITIR
                     NYGLSDASEIFIFISGYTASLVYGRAMQQHGFVIAAARILRRCWQIYVAHLFLFVFYI
                     AEITNAASRFHEPLFAEETHVLAFLQHPDATIVQALLLKFKPVNLDVLPIYILFLLAF
                     PAVLWLLRRNLALPLAASAVLYALTWMFQWNLPAYPAGEWFFNPFAWQLLFVFGAWCA
                     AGGAEYLQALMRSRITAVLAVSYLLFGFLLAGSWHWHWMVRIEPDWLIRLVVAHPIDK
                     TNLHALRLLHFLALALLVLRVFPRHWPVLTSPALRPAILCGRHSLETFCLGTFLAFAG
                     HFAIVEIAHGIGAQIVISSLGIALMVGLAGLIDWYQATEASARHAPPGVAPANPRRER
                     PARSVAMVLFHRIRKTAGASSRSRTMNIRLGIPNYSAALSRFAGRPFSSRDRR"
     gene            complement(4750..5793)
                     /locus_tag="VGA15_13785"
     CDS             complement(4750..5793)
                     /locus_tag="VGA15_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012754135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9588799.1"
                     /translation="MRQTVKSDSPMEFAKIAGTTLEVSRIGLGTWAMGGWMWGGTDER
                     ESIATIHAAIERGINLVDTAPAYGFGRSEEIVGRALGQGGLRRQVKIATKVGLEWKDG
                     KVFRNAGRDRILREIEASLQRLRTDYIDIYQVHWPDPLVPVEQTAEAMRTLLEQDTIR
                     AIGVSNFSIDQIERFRRVAPLHVVQPPYNMLERGIETELLPYCRETSLATLTYGALCR
                     GLLSGKMQPDSRFDGDDLRLVDPKFRQPRYSQYLAAVRRLDQLAQQRYRKRVIHLAVR
                     WILDQGASVALWGARHPAQIEPVDEIMGWSLDAASKSEIDRILRELVTDPIGPEFMAP
                     PQRPTIDALPALL"
     gene            complement(6414..6815)
                     /locus_tag="VGA15_13790"
     CDS             complement(6414..6815)
                     /locus_tag="VGA15_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588800.1"
                     /translation="MKTKLAIAAVALLAGTLMLPGQEVLARGGGGGGGHGGGFGGGHG
                     GGFGGGGFHGGGIGGGFHGGGFGGSGFRGGGFGGRGVGGYGRGYGYGRGYYGYGRGYG
                     YGRGYGYYGGYGGYGGYGCDPFYYDYYGCYY"
     gene            7042..7281
                     /locus_tag="VGA15_13795"
     CDS             7042..7281
                     /locus_tag="VGA15_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX9588801.1"
                     /translation="MSDARYYIVGDHDVWMIKFEDGDYGPYAGRDEAVVFAIDAAQRL
                     STRGECAHVCVVDDDGRFRSKWTYDRDHHLRRSAS"
     gene            7473..7703
                     /locus_tag="VGA15_13800"
     CDS             7473..7703
                     /locus_tag="VGA15_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588802.1"
                     /translation="MHTTTNITLAAALVLGCVSTASAANYTGHHGHAYATGTLSPNWA
                     GGRQVAAPPWSFACMTDHGPSPCGEPMWVYRN"
     gene            complement(7994..>8183)
                     /locus_tag="VGA15_13805"
     CDS             complement(7994..>8183)
                     /locus_tag="VGA15_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588803.1"
                     /translation="LAMVDVREASRVRGFERRLVADRVLAIAFFLAVFAVEIAIVQFA
                     GLEPPLALDAQPIVVPIT"
BASE COUNT         1613 a         2572 c         2545 g         1453 t
ORIGIN      
        1 accttgcgac gccgagcagc ctggaaatcc gaatttcaag cctacgccag taacgccgtt
       61 tgttgaggcg tgaactttcg agtgcagcaa gcattgcagc cgacaggaac ggcaagctct
      121 gcaccaacag tgctccggcg aagaaataat tggccctgat ctggtcgtaa ttggtgatcc
      181 acacggcggt cgcgccagcc acgagcagcc cgcccatcgt gacctcccag aacgccgcaa
      241 atccgacgct gcggtcgcct ctaccgcctt tggcagtgcg gtggaatcgc ttctgatcac
      301 gtcctatcgc tgcgtgagcg atcgcgttgg ccacggtcca ttgcaccgac atggccgcga
      361 acagggcgcc agctgcggct cggagcggac tatcgactcg cagccgatac gcgatcgaaa
      421 aatgcagcag cgaaagcatg aaggcaacga tcgtcggcag catcaggatc gtgtcaggga
      481 tggcgaggcc aaagaagatg acgagcggga cccacacgag attgagcatg gcggcggcaa
      541 cggcgatggt ttcgccgccc agccagcgca accagccgat cacgaactcg cgcttctggt
      601 cgtggtcgag ccggcttgcg ccgggcagga agcggcgcca atgcttcttc aggatctgcg
      661 ctccgccaca ggcccagcga tagcgttgtt tcttgaacgc ctcgtagctc tccggcagta
      721 gtccccatcc atagcgccgg ttggtataat gggcacgcca gccgagttcc aggatggtga
      781 ggccgaggtc agtgtcctca cagatcgtgt cgctcgacca acggcccgcg gcctcgagcg
      841 cggcccgtcg aatcaagcac atggtgccgt gcataatgat ggcattggtc tcgttgcgct
      901 ggaccatgcc gacgtcgaag aagccggcat attcgctatt catggcggcg tgcacgatgc
      961 tgcgatcacc gtctcggtga tcctgcggtg cctgcacaag cgccacgctc gaatcgtaga
     1021 aggtcgggat gagatctttc agccagtctg gatgcaccac ataatcggca tcgagcacgc
     1081 cgatgatctc agcatcggca gccgtgttcg ccaaagcaag gttcaaggca ccggctttga
     1141 agccttcgag cttctccacg tagataaatt tgaagcgctc gccgagagct cggcaatact
     1201 cctcgatcgg acgccacaac gagggctctg gcgtgttgtt gatgaccacc acgcactcgt
     1261 aattcgaata acgcaggcgt gcgaccgcat cgagtgtgag cttgagcata tccgcaggtt
     1321 cgcggcgggc cgcaatatgg atcgagacct tcggcgcgaa gccctcgcgc gctgccaccg
     1381 aggcaggcag catccggcgc ggctccggtc ccaacgcgac cgcggcaagc tcttccaatc
     1441 gcgccagcgc catcgcgatc aggagcgcca gcagggcgag ccccggcccg aacgcgaccg
     1501 cctcgccggg tgtgaaataa tggctgtgcc agtacgccag cacggtcgcg caccaggcgc
     1561 cagccgcatg tgccgagatc gcgagcaccg cgacttgtcc gaacgtgccg ccggacagcg
     1621 cgaaaaccgg caacgacaac aggattccac cgagaactgc gactgctgct gtcctccaac
     1681 gatcggcgtt gccgacggct ccaacccagg cgaatttggg ctgcagcgag gcgctgaata
     1741 tgccccaata cggccccacg ctgccctcga agaccttccc cggctggtcg attgcttcga
     1801 cgatgttgta atcgatgccg gccgcgtccg cgcgcgtgac gaagtcgcgc agcactttgg
     1861 cctgctcgcc gcgccccggc atggcgttct ttatgttgtg cccgccgctc ggccagccga
     1921 attcgccgat aacgataggc ttgcccggaa aggctgcgtg cagcttgtta tagatgagca
     1981 gcgcctgatc gaccgcgatc ttttcggaga atccttccca gtacgggatg atgtgggcaa
     2041 agatgcagtc aaccgcgttt gcaagctcgg ggtgctcgag ccagacgggc cattgttcgg
     2101 cggtggtgac cgggaccggg ctctcgcgtt tgacgcgctc gagcagtcga atcatgtagt
     2161 cggccgtatg ctcgcggcgg aagatcgttt cgttgccgac gatgagccgc gtgacattgg
     2221 ggttctgtcg ggcaacttga atggacgaac ggatttcgcg ttcgttgcgc gcctcgtcgt
     2281 tgccgatcca ggcgcccatg ccgacattga gcccgaactc attggcaatc gcggggacga
     2341 gctcgaggcc gccggtcgag gcataagtgc gcacggcctt tgtctgacgc gcgacaacct
     2401 cgaggtcgga gcggatctgc gcggcagtca cccgcccgcc attatccgaa gcttccaatc
     2461 gcgtatagga aagactcggt agcggaccct gaacattagg ggcagccaac tcgccggaat
     2521 tcagcgccca taggaatgca tgcacggacg cgaccagcgc cactgcgact gcaacgcgac
     2581 gaatggagtg acccggattt gcgcgagcaa tgcgaggcat ggagtcatcc attcaaactc
     2641 attgccgggt cttgcgttca tggggcgcac gccgagcgct ttcgtaacgg cggacggtag
     2701 tgtcgtaccg gcaagggccc acgtagttcc cggcacccgc tgatctatgc ggtcctgcgg
     2761 ccacgcgctt ggtcgggctt gctgtgttct tggctatgtt gcaacaatcc gcacccgcgc
     2821 ctgtttcggc tatcgggcgc gcctgccaag ccatgctccc cattctatcg gcaaagtcgg
     2881 gccatttcgc gacaatcacg ggcaagcggc caggcgcttt tccgcctatt ggggggctga
     2941 gtttcatcgg cgcgcctcaa aggggagaca ttccatgaat cccacacggt cgcacgacac
     3001 gatccgaaat cgcgccgcgc ccattcggcg gctggcgctg gtgatgggcg ctgccgcccg
     3061 attggcggcg cctcttttgc ggcgccggca gatagcaatc ccgcgcgggc atctcccgcc
     3121 cccagcatgg acccgcgatt ggaggcgccg atcggccatc gccagccgaa cacggaggat
     3181 gtacccgcaa gcgtgttgca cgacgaaggt acaacaaacc agaaccaacg cgatctcgac
     3241 aagaagctca acgtctgtcg cggctgctaa aggggcgccc ggcaaatcgg ctaagcgctg
     3301 cgctgtaatt ggggataccg agcctgatat tcatcgttct cgagcggctc gatgctccgg
     3361 cggtcttgcg aattcgatga aatagcacca tggcgaccga tcgcgccggc cgttctcttc
     3421 ggggatttgc cggtgcgacg ccgggaggcg catgccgcgc ggatgcttcg gtggcctgat
     3481 accagtcgat gagcccggca aggccgacca tcagcgcgat tcccaggctg ctgatcacaa
     3541 tctgcgcccc gatgccgtgg gcgatttcga cgatggcgaa gtgcccggca aaggccagga
     3601 aggtgccgag gcaaaaggtc tccagcgaat gccgtccgca cagaatggcg gggcgcagag
     3661 ccggcgatgt cagcaccggc cagtggcgcg ggaacactcg gagcaccaaa agggcaagag
     3721 ccagaaaatg cagcaggcgc agagcgtgga gattcgtctt gtcgatcgga tgcgcaacta
     3781 ccagccggat gagccagtcg ggctcgattc gcaccatcca atgccaatgc cagctcccgg
     3841 cgagcaggaa cccgaacaga agatagctca ccgcaagcac cgcggtgatg cgcgagcgca
     3901 tgagagcttg caggtattcg gcgccgccgg ctgcgcacca cgcgccgaac acgaacagca
     3961 gctgccacgc gaacggattg aaaaaccact cgcccgccgg atacgccggc aagttccact
     4021 gaaacatcca ggtcagcgcg tacagcacgg ctgacgccgc gagcggcagc gccagattcc
     4081 ggcgcagcag ccacagcacc gccggaaagg ccagcagaaa caggatatag atcggcagca
     4141 cgtcgagatt gaccggcttg aacttgagca gcagcgcctg gacgatcgtc gcatcgggat
     4201 gctgcaggaa agcaagaacg tgcgtttctt cggcgaacag cggctcatgg aaccgcgacg
     4261 ccgcgttggt gatctcggcg atgtagaaga cgaacaggaa caggtgggcg acgtaaatct
     4321 gccagcagcg ccgcaggatc cgcgccgccg cgatgacgaa cccgtgctgc tgcatcgcgc
     4381 ggccgtagac cagcgaggcc gtatacccgg aaatgaagat gaagatttcg ctggcgtcgc
     4441 tcaagccgta attgcggatc gtgatccagc tgacgacgtt cgagggaatg tgatcgagaa
     4501 agaccagcca gagcgccagt ccgcggaaga aatccagacg caagtcgcgc ccgggaatgg
     4561 cagatcgccg tgcaacggac agcatgggta acgctcttgc cggtagcggg cgatccggac
     4621 cttgcaggtg tgcggggaaa gcggcggcgc cggtttcccc gggggtccat ggcggtcgat
     4681 aggcgcccta tgcgaagctg cggcgatact ggctgaggct cgttccgacc ggcttgcggg
     4741 ccgcgggcat tacaagagcg ccggaagggc gtcgatggtc ggtctttgcg gcggcgccat
     4801 gaactccggg ccgatcgggt cggtgacgag ctcccgcaag atgcggtcga tctcggactt
     4861 cgacgcggcg tcgagcgacc atcccatgat ctcgtccacc ggctcgattt gagccgggtg
     4921 ccgtgcgccc cacagcgcca cgctggcgcc ctgatcgagg atccagcgca cggcgagatg
     4981 gatgacgcgc ttgcggtagc gctgctgggc cagctggtcg agacgccgga ccgcggcgag
     5041 atattgcgaa tagcgcggct gccggaattt cggatcgact aggcgcaggt cgtcgccatc
     5101 gaaacgcgaa tccggctgca tcttgcccga cagcagcccg cggcacagcg cgccgtaggt
     5161 cagcgtcgcg agcgacgtct cgcggcaata gggcaggagt tcggtctcga tgccgcgctc
     5221 cagcatgttg tacggcggct gcacgacgtg aagcggggcg acgcggcgga atcgttcgat
     5281 ttgatcgatc gagaaattgc tcaccccgat ggcgcggatc gtgtcctgct cgagcagcgt
     5341 ccgcatggct tccgccgtct gctccacggg caccagcgga tcgggccagt ggacctgata
     5401 gatatcgatg taatccgtgc gcagccgctg cagcgaggcc tcgatctccc gcaggatgcg
     5461 gtcgcggccg gcgttgcgga agaccttgcc gtctttccac tcgaggccga ccttggtggc
     5521 gatcttcacc tgccgacgca gcccgccttg gccgagggcg cggccgacga tctcttcgga
     5581 gcggccgaag ccgtaagccg gagcggtatc gaccagattg atgccccgct cgatggcggc
     5641 gtggatcgtc gcgatcgact cccgttcatc cgtcccgccc cacatccagc cgcccatcgc
     5701 ccaggtcccg agtccgatgc gtgatacttc gagcgtcgtc ccggcaatct ttgcgaattc
     5761 catagggctg tcggacttta cggtctgtct catcatcggc tccaatcatt atcggttcga
     5821 gttgcatcgg tcggccgtca gccgcccggc atagctccaa agacccggct gaacgccgcg
     5881 tccgatctgc cccgttctgc cgagacttag gtcttcttct cgcaactcgt ttgttcggaa
     5941 ttgccgcgtt ttgcgcttgg ctgctgcgat ctggttggca cggacgacat cggcacggtg
     6001 ggtcgcaaac ggatgaaggt tcgagctgca ttgaaccgtg accgcccttc tggaggctct
     6061 tcttcgacgg aggacgcgca ataacgcaag atcacatcaa ggagggcagt aatcgccaag
     6121 ccggcattgt gcggtccgta taggtccaca accgaacctc atgaaaggag atcaacatgc
     6181 ggaccttcac tttcattgcg tttgcttgct tgtcggtcgc cttcgtgggc ggcactcgtg
     6241 ccgacgctgc cgatcgggag gcgtggtgtg catattacga ggacaacgcc ggcacgaact
     6301 gcggttttgc gacacttgag caatgtcaag ccgacatcag cggggtcggc ggatactgcg
     6361 cgccaaatcc ggacgcaggt cagtaatccg cacgaccggg ccggacacga tccctaataa
     6421 tagcacccgt agtagtcgta gtagaaggga tcgcagccat acccgccgta accaccgtaa
     6481 ccgccgtagt atccgtagcc gcgcccatat ccgtagccac gcccgtaacc ataatagccg
     6541 cgcccatacc cgtagccgcg gccatagccg ccaactcccc gaccgccgaa tcccccgccg
     6601 cggaacccac ttccgccgaa gccaccaccg tggaaaccac caccgatgcc gcctccgtga
     6661 aagccacctc cgccaaagcc cccgccgtgg ccgcctccaa acccgccgcc atgtccgcct
     6721 ccacctccgc caccgcgggc caacacctct tggccgggga gcatcagtgt gccggccagc
     6781 agcgcaacgg cggcaatcgc caatttcgtc ttcatcacat tctctcctgt cgcaacggcg
     6841 agtcgcgatc gacccgaacc tgatgcacag gagacgggat gagatgcgag cttatttccc
     6901 agtggcgcgc acgagttcgt gaaggtgaga atttgtttgg atgtgatctg tgtcacatct
     6961 ccgcgttcgc gtaatcctct atctggattt gtcgcgaccg tcggcgtcaa ctgccttcgg
     7021 agagtcctca gggagagaat catgtcggat gcccggtact atattgtggg agaccacgac
     7081 gtgtggatga tcaagttcga agacggcgac tacggtccct acgcgggccg cgacgaggcg
     7141 gtcgtcttcg caatcgatgc cgcgcaaagg cttagcacgc ggggcgaatg cgcccatgtg
     7201 tgcgtggtgg atgatgacgg ccgctttcga tcgaaatgga cctacgatcg ggatcatcat
     7261 ctgcgcagga gcgcgtcata ggctttagcg ccgccccggc gcggcgacct caatggatgc
     7321 caagccgtcg ggagatgaga ttccccgggc gaactccgta ggcatccccg cgttcaccag
     7381 tttgtcatca gcttgttacc ggtcgcgccg aggagcagct ttacgggaat ggaattcaaa
     7441 caattcggtc ttctcaacga aggagatcga cgatgcatac caccaccaat attacgcttg
     7501 ctgctgcgct tgtgctcggc tgcgtttcca cggcatcagc cgcgaattat accgggcacc
     7561 acgggcacgc ctatgcaacg ggaacgctgt cgccgaactg ggccggggga cgccaggtcg
     7621 cagccccgcc atggagcttc gcgtgcatga ccgatcacgg tccaagcccg tgcggtgaac
     7681 ccatgtgggt ctacaggaat taggatccgt aagcgcacaa cgcctggtca atgtcggcca
     7741 tcgtgcgatc cggttggtcg accagcaagg tccgcttccg tctgtgcacc cggtcatttc
     7801 cttgtgcccg tcgtggctgg gttcgcacgc ggccggtcca ctcggcggga ccggccgctc
     7861 tcgttgggcg aacaggtcag cgaaatcgct gcgctgagca gacgatcctg tcctatttcg
     7921 catcctctgg agacggcatt tcagttgaaa ttgctggcct tccgctcatc tgcaagctcg
     7981 gcagtgagtt ccgctaagtg atgggcacaa caatcggctg ggcgtcgagg gcgagcggcg
     8041 gctcaagtcc tgcaaattgc acgatggcga tctcgactgc aaacaccgcg aggaagaaag
     8101 cgatcgcaag tacgcgatca gctacgaggc gcctttcgaa accgcgaacg cgcgaagcct
     8161 cgcgaacgtc taccattgcc agc
//
LOCUS       DASWGM010000481         8612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2411596,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000481 DASWGM010000000
VERSION     DASWGM010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2411596"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1534
                     /locus_tag="VGA15_13810"
     CDS             98..1534
                     /locus_tag="VGA15_13810"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX9588804.1"
                     /translation="MSFVERHGLWSSEQKEAASRLRKIVEEQKLEVIRLSFPDQHGIL
                     RGKTLVAREALRTLESGCSITTTMFAKDTSHRTVFPVFTAGGGFGMTEMEGAADVLMV
                     PDPLSFRVLPWASATGWLLCDVHFADGRPVPFATRNLYKSVLAGLARRGYDFVAGLEV
                     ECHIFKLEDAKMSPEDAGQPGQAPSVSLLSHGYQYLTEQRYDQMEPVLDLVRRDIQAL
                     GLPLRSIEVEFGPSQCEFTFQPTMGLEPADNMVLFRSAVKQICHRHGYHATFMCRPKL
                     PNVFASGWHLHQSLVSRASGENAFMSKEGGEILSPFGRCYLAGLLRHARASTVFTTPT
                     INGYKRYRSYSLAPDRAIWGRDNRGVMIRVLGGADDPATRLENRIGEPAANPYLYMAS
                     QILSGLDGVDRELDPGPSADTPYETKAALLPKTLQDAVLALKDDPFFRDALGAGFVDY
                     YVHIKNAEIERFQAEVSDWEQREYFEMF"
     gene            complement(1671..2729)
                     /locus_tag="VGA15_13815"
     CDS             complement(1671..2729)
                     /locus_tag="VGA15_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9588805.1"
                     /translation="MKQFRNHVIRAGLGALYFSGAHHLLRPIFAGVGAIFMLHHVRPR
                     RDAEFQPNRHLEVAPEFLRAMLAHLRARDVDIVTMDELHQRLIDGNFSRRFACFTLDD
                     GYRDNRDFALPVMREFDAPSTVYVASDFAQGSGRLWWIALETVVARASAIDVAIGGAA
                     TRLDTATPANKQAAFDRLHDWLRGLPGEHDIEREIGALCARHGVDEIAICRELCMSWD
                     ELKPFADDPLITIGAHTITHCNLARQPEAIAAHEMASSRARIESVLQRPVVHLAYPYG
                     DKIAAGPREFALAHASGYKTAVTTRPGMMFPESAGHLTALPRVSLNGNYQDTRILPVL
                     TSGAATAMWNGFRRIDAA"
     gene            2897..4291
                     /locus_tag="VGA15_13820"
     CDS             2897..4291
                     /locus_tag="VGA15_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX9588806.1"
                     /translation="MHGLFRWRSKWWPGLIPLAIFWAIAAWTSTSTVESDLAARSTAA
                     LKDTVLDKTRIAVAGRDVTLAADAFSEDGRGSAAAAVQAVPGVRRINDETRLVPEAKP
                     FVWSAERDVVRVTLGGSAPLPASKVKLVEAARASLGGVEVADQMNLSRGAPPRFDDAA
                     SLLLDQIGKLKDGKITISDSKVSLSGMARELGGREAIAAALKNLPEGFSVAANEIKAP
                     PYIFQAYKDPVAVTLTLTGYVPDNNVHAALAAAAGRKFFSEKVVDNLKASVGAPSGFA
                     NAVMPALGALSRLSTGTLVVSDREVKVSGDALYEAAAVQTRAGLGKDFPQSWQFKADI
                     SVKPQAAPVDATVCQQLFSDLLAKGNIRFESGRATIDPDSVGLLDRLVETALRCPAAN
                     IEIAGHTDADGEDGFNQMLSEKRAQAVVDYLVKAGLPASQFTATGYGSTQPVASNDTD
                     EGKAQNRRIDFLVR"
     gene            4298..4594
                     /locus_tag="VGA15_13825"
     CDS             4298..4594
                     /locus_tag="VGA15_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588807.1"
                     /translation="MAYLVTFHWGWLLASLLLGFAMGWIAVVHRGPGVSKRTSLWLAV
                     LVAALVAAALARVVPGRPGYWLDLGLIMFACYLAGCAVGSWLRDWVLSRHTQAA"
     gene            4630..5145
                     /locus_tag="VGA15_13830"
     CDS             4630..5145
                     /locus_tag="VGA15_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX9588808.1"
                     /translation="MTPSAHYRMFGHYNAWANGRLYDATAHLSTEQYRADRGAFFKSV
                     HGTLNHLLVTDRIWMQRFTGEGDTPSRLDAILFERFDDLRAAREAEDRRIIDFVDGLD
                     DGRLAGTIKYRRVSSPEEFEQQLAPALAHWFNHQTHHRGHVHALLTGLTGEAPELDLL
                     IFQRLSAKSAT"
     gene            5403..6458
                     /locus_tag="VGA15_13835"
     CDS             5403..6458
                     /locus_tag="VGA15_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HEX9588809.1"
                     /translation="MLEAIRRAISFLRQKQILHKLGVVVSITVIGIACYVLYHMLRGI
                     DTNEVIEAIKQTESRQIVLAGVFVAAGYFTLTFYDLFAVRAIGRSDIPYRINALAAFS
                     SYSIGHNVGASVFTGGAVRYRIYSAWGLNAIDVAKICFLAGLTFWLGNAAVLGLGIAY
                     HPEAAAAIDQLPPWLNRIAAFAIIVGLVAYVVWVWTQPRRVGRGPWTVVLPGGPLTLL
                     QIAIGIVDLGFCALAMYVLVPDEPNLGFVVVAVIFVSATLLGFASHSPGGLGVFDAAM
                     LVGLWQMDREELLAGMLLFRVLYYLAPFVISVILLTFREVIMGARLKRLRRARPGLDA
                     EPKREAVYVRERGDTGA"
     gene            6492..7790
                     /locus_tag="VGA15_13840"
     CDS             6492..7790
                     /locus_tag="VGA15_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9588810.1"
                     /translation="MAIDESSSSFFSPWPDRLRHSAIILLAAGLALAVLVTFGELSLV
                     RAAAVFICIGAAALVPWRLHNAVASREDVRGVNPVEAAAVSAVVAGMPDPAVLLDRAG
                     RVIHLNAAAAQLAPALRKNELAQFALRSPEIITALREAIATTESRRATYLDHVPVDRW
                     MELIITPVPVPTLFGGIDKCMLMTFHDQTPLRRVEEMRADFVANASHELRTPLAALSG
                     FIDTLQGPARDDAKARERFLGIMHTQATRMARLIDDLLSLSRVELSAHVRPDTVVDIV
                     PIIRQVVDGLEPLAGDRQVAIDIQLPDRPVVIAGDREELLRLFENLIENALKYGASGG
                     KIIVSLTSAMSGEGLPEIRVMVRDFGPGIAPEHLPRLTERFYRVDVGDSRAQGGTGLG
                     LSLVKHIVNRHRGRLLIESVPKNGATFTACFPQLRSPAAV"
     gene            7930..>8612
                     /gene="pstS"
                     /locus_tag="VGA15_13845"
     CDS             7930..>8612
                     /gene="pstS"
                     /locus_tag="VGA15_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX9588811.1"
                     /translation="MNFVKAIVAAGLVAASTSAFAADITGAGATFPFPIYSKWADAYK
                     KETGNGLNYQSIGSGAGIKQIEAKTVTFGATDAPLKAEQLEKDGLVQWPMVMGAIVPV
                     VNLEGIKPGELVLSGEVLGDIYLGKITKWDDPAIAKLNAKLKLPTDAITVVRRSDGSG
                     TTFNFTDYLSKSNPDWKTKVGSGTAVEWPAGVGAKGNEGVAGNIGQTKNAIGYVEYAY
                     AKQNKLTYAA"
BASE COUNT         1541 a         2654 c         2771 g         1646 t
ORIGIN      
        1 ccggccgaag gccggacccg ttggctcctc gcaatgacga acagcacata tttcccttaa
       61 ctgcagcttg cgagttggac tccaaggggg caacgcgttg agcttcgtcg agcgtcacgg
      121 tctctggtcg agcgagcaga aagaagcggc gagccgtctg cgcaaaatcg tcgaggagca
      181 aaaactcgaa gtcatccggc tgtcgttccc cgatcagcac ggcatcctgc gcggcaagac
      241 gctggtcgcg cgcgaggcgc tgcgcacgct ggagagcgga tgcagcatca ccaccacgat
      301 gttcgccaag gacacctcgc accgcaccgt atttcccgtc ttcaccgccg gcggcgggtt
      361 cggcatgacg gaaatggaag gcgccgccga tgtgttgatg gtgccggatc ccctgagctt
      421 tcgcgtgctg ccgtgggcgt cggcgaccgg ctggctattg tgcgacgttc atttcgccga
      481 cggccggccg gtgccgttcg cgacccgcaa cctctataaa tcggtgctgg ctggcctcgc
      541 caggcgcggc tacgatttcg tggccggcct cgaagtcgag tgccatatct tcaagctcga
      601 agacgccaaa atgtcgcccg aggatgccgg ccagccgggt caggcgccgt cggtcagcct
      661 gttgtcgcac ggctatcaat atctgaccga gcagcgctac gatcagatgg agccggtgct
      721 cgatctggtc aggcgtgaca tccaggcgct cgggctgccg ctgcggtcga tcgaagtcga
      781 gttcggtccg agccagtgcg aattcacctt ccagccgacc atgggccttg agcccgccga
      841 caacatggtg ctgttccgca gcgccgtgaa gcagatctgc catcgccacg gctatcacgc
      901 gaccttcatg tgccgcccaa aattgccgaa tgtgttcgcc tccggctggc atctgcacca
      961 atcgctggtc tctcgcgcca gcggtgagaa tgctttcatg agcaaagagg gcggcgagat
     1021 attgagcccg ttcggccgct gctatctcgc aggactgctg cggcatgcgc gggcctcgac
     1081 cgtattcacc acgccgacca tcaacggcta caagcgctat cgttcctatt cgctggcgcc
     1141 ggatcgcgcg atctgggggc gcgacaaccg cggcgtgatg atccgcgtgc tcggcggcgc
     1201 ggatgatccc gcgacccgtt tggaaaaccg gatcggcgag cctgccgcca atccctatct
     1261 ctacatggcc tcgcaaattc tctccgggct cgatggcgtt gatcgtgaat tggatcccgg
     1321 cccatcagcg gacacgcctt atgaaacaaa agccgccctg ctgccgaaga cgctgcagga
     1381 cgcggtgctg gcattaaagg acgatccgtt ctttcgcgac gcgctgggcg cggggtttgt
     1441 cgactactac gtccatatca agaacgccga aatcgagcgc tttcaggccg aagtatcgga
     1501 ttgggaacag cgcgaatatt tcgagatgtt ttgaacgggc gacgcgcatt ccacaaagca
     1561 ccactgcttc agcgttgact gtgcgctccc tcccccctgc tgggcccccg caagggggga
     1621 gggaatagaa gtgcaacgct gcccggtatg tcctgtgcag cgtctaaccg ctacgccgcg
     1681 tcgatgcgcc gaaaaccgtt ccacatcgca gtggcggcgc cagaggtcag caccggcaaa
     1741 atgcgggtgt cctgatagtt gccgttgagc gaaacccgcg gcagcgcggt cagatggcca
     1801 gcgctttcgg gaaacatcat gcccggccgt gtcgtcaccg cggtcttgta tccgctcgcg
     1861 tgcgccagtg cgaactcgcg ggggccggcg gcgatcttgt cgccataggg gtaggcgaga
     1921 tggaccaccg gacgctgcag cacgctttcg atccgcgcgc gactgctcgc catttcgtgg
     1981 gcggcgatcg cctcaggttg tctggcgaga ttgcaatgcg tgatcgtatg cgcgccgatc
     2041 gtgatcaggg gatcgtccgc aaacggcttc aactcgtccc acgacatgca cagttcgcgg
     2101 caaatcgcaa tttcatcgac gccgtgacgc gcgcacagcg caccgatttc gcgctcgata
     2161 tcatgctcgc ccggcaatcc gcgcagccag tcgtgcaaac ggtcgaacgc ggcctgcttg
     2221 ttggccggcg tagcggtatc gaggcgggtc gcagcacctc cgatcgcaac gtcgatcgca
     2281 gaggccctgg cgaccaccgt ctcgagcgcg atccaccaca gccggccgga gccttgcgcg
     2341 aaatcgctgg cgacatagac cgtcgaaggt gcgtcaaatt cccgcatcac cggcagcgca
     2401 aaatccctgt tgtcgcgata gccatcgtcg agggtgaagc aggcaaaccg tcgcgaaaaa
     2461 ttaccgtcga tcaaccgctg atggagctcg tccatggtga cgatgtcgac gtcacgcgcg
     2521 cgaaggtgcg cgagcatcgc gcgcaggaac tccggcgcga cctcgagatg acgattgggc
     2581 tggaattcgg cgtctcggcg cgggcggacg tggtgcagca tgaagatcgc gccgacccct
     2641 gcgaagatcg gtcgcagcag atgatgggcc ccgctgaagt atagcgctcc cagcccggcg
     2701 cggatgacgt ggttgcgaaa ctgcttcatg ctggagtagc tagccggttg ggcgagattc
     2761 cgaagctaac gggagaccgc tgaagaaaca gttagtccga aaggctgtcc tgcgccggac
     2821 tattttcgtg ttatttccag tttgacagcc tcgcaagggt ttgttttctc agagttccag
     2881 acccgcgaga cgtcgaatgc acggactttt caggtggcgc agcaaatggt ggccgggctt
     2941 gattccactg gccatctttt gggctatcgc ggcctggacc agcacctcaa ccgtggaatc
     3001 cgatctggcg gcgcgcagta ccgccgcgct gaaggatacc gtgcttgata agacgaggat
     3061 cgcggtcgcc ggccgcgatg tcacgttggc ggcggatgca ttctcggaag acggccgcgg
     3121 cagcgcggct gcggcggtgc aagctgtgcc cggcgtgcgg cgcattaacg acgaaacccg
     3181 cctcgttccc gaagccaagc cgttcgtctg gtcggctgag cgggacgtcg tcagggttac
     3241 gttgggcggc agcgcgcctt tgccggccag caaggtcaag ctagtggagg cggcccgggc
     3301 aagtctcggc ggcgtcgagg tcgccgatca gatgaacttg tcgcgggggg cgccgccgcg
     3361 cttcgacgat gcggcctcgt tgctgctcga tcagatcggg aaattgaagg acggcaagat
     3421 cacgatttcc gatagcaagg tcagcctttc cggcatggcg cgcgaactgg gcggccggga
     3481 agcgatcgcg gccgccctga aaaacctccc ggaggggttt tcggtcgccg ccaacgagat
     3541 caaggcgccg ccctacatct tccaggccta caaggatccg gtcgcggtca ccctgacgct
     3601 gaccggctat gtacctgata acaatgtcca tgcggcgctc gcggcagccg ccggacgcaa
     3661 attcttcagc gaaaaggtgg tcgacaatct caaggccagc gtgggcgcgc catccgggtt
     3721 cgccaatgcg gtgatgccgg cgctgggcgc gctgtcccgg ttgtcgacgg gtactttggt
     3781 agtgtccgac cgggaggtga aggtctcggg cgacgcgctc tatgaagccg cggcggtcca
     3841 gacgcgggcc ggtctcggca aggattttcc gcaatcctgg cagttcaagg ccgacatttc
     3901 ggtcaagccc caggcagcac cggtggatgc caccgtatgc cagcagctgt tttcggattt
     3961 gctggccaag ggaaatatcc ggttcgagtc cgggcgcgcc accatcgatc cggattccgt
     4021 ggggctgctc gaccgcctgg tcgaaaccgc gttgcgctgc ccggccgcca acatcgaaat
     4081 tgcgggacat accgacgccg atggcgagga cggtttcaac cagatgctgt ccgaaaaacg
     4141 cgcgcaggcg gtggtcgatt atcttgtcaa agccgggttg ccggcgagcc agttcaccgc
     4201 gacgggctac ggcagcacgc agccggtcgc gtcgaatgat accgacgaag gcaaggcgca
     4261 aaaccgccgc atcgattttt tggtgaggta ggggcgaatg gcatatctgg tgacatttca
     4321 ttggggctgg ctgctggcgt cgctgttgct gggtttcgcg atgggctgga tcgccgtggt
     4381 tcatcgcggt ccgggcgtat cgaagcggac gtcgctttgg ctcgcggtgt tggtggccgc
     4441 acttgtcgcg gcggcgctcg cacgcgttgt tcccggccgc cccggctact ggctcgacct
     4501 cggcctcatc atgttcgctt gctatctggc cggttgcgcg gtgggatcgt ggctgcgcga
     4561 ctgggtactg tcacgtcata cgcaggcggc ttgagagctt ccggtcgcca acaggagact
     4621 tgagtatcca tgactccctc ggcccattac cggatgttcg gtcactacaa cgcctgggcc
     4681 aacggccggc tgtatgacgc gacggcgcat ctctcaaccg agcaatatcg ggccgatcgc
     4741 ggcgcctttt tcaaatcggt tcatggcacc ctaaatcatc tgctggtgac cgaccggatc
     4801 tggatgcagc ggtttaccgg cgagggcgac acgccgagcc ggctggacgc gatcctgttc
     4861 gagcggttcg acgatctgcg cgccgcgcgc gaggcggagg accggcggat catcgatttt
     4921 gtcgacgggc tcgatgatgg gcgcctcgcc gggacgatca aataccgccg cgtctcgtcg
     4981 ccggaagaat tcgaacagca actggcgccg gcactggcgc actggttcaa tcaccagacc
     5041 caccaccgtg ggcatgtcca cgctttgtta accggtctga caggcgaagc gcctgaactc
     5101 gatctgctga ttttccagcg gctatcggca aaatccgcca cctgacatca cgaaaacttc
     5161 accgcttgac gagcaaggtc gcggtcaggg actttgcgtt gccgcaggga atgcagcaaa
     5221 actgtgcttc cggcgtcatg aacactccct aatatgttga agaagcgcgt tttattcacg
     5281 ccaagcgaat tccactgccg ctcaaaacgc gctacgacat cctgtccggg accaagcgta
     5341 gcgccggcgg cggcagggct cgcacggcag ccgtcgtcgt gggatcgcag ttcgaggtgg
     5401 agatgcttga agcaatacgc agggcgatat cgtttctgcg ccagaagcaa atcctgcata
     5461 agttaggtgt tgtggtcagc atcacggtca tcgggatcgc gtgctacgtg ctctatcata
     5521 tgctgcgggg catcgatacc aacgaagtca tcgaggccat caagcagacc gagtctcgcc
     5581 agatcgttct ggcgggggtg ttcgtggcgg cgggttactt caccctgacc ttctatgacc
     5641 tgttcgcggt tcgcgcgatc ggccgcagcg atattcccta ccgcatcaac gcgcttgcgg
     5701 ccttcagcag ttattcgatc gggcacaatg tcggcgccag cgtcttcacc gggggcgcgg
     5761 tgcgctatcg gatttattcc gcgtggggat tgaatgcgat cgacgtcgcc aaaatctgct
     5821 ttctggccgg cctgacattc tggctcggca acgccgcggt gctggggctg ggtatcgcct
     5881 atcacccgga agcggcagcc gcgatcgatc agcttccgcc ctggctgaat cgcatcgccg
     5941 cgttcgccat cattgtcggg ctggtggcct atgtggtctg ggtctggacc caaccgagac
     6001 gggtcggccg cgggccgtgg accgtggtgc tgccgggcgg tccgctgacg ctgctgcaga
     6061 tcgcgatcgg cattgtcgat ctcggctttt gcgcgctggc catgtacgtt ctggtgccgg
     6121 acgaacccaa tctcggcttt gtcgttgtcg cggttatttt tgtctcggcg acgcttttgg
     6181 gcttcgccag tcactccccc ggcgggttag gggtgttcga cgccgccatg ctggtcggct
     6241 tgtggcagat ggatcgcgaa gagctgctcg cggggatgct tctgttccgc gtcctctatt
     6301 atcttgcgcc gttcgtcata tctgtaatct tgctgacgtt tcgggaggtc ataatgggcg
     6361 ctcggctgaa gcgcctgcgc cgggcgaggc cggggctcga tgccgagccg aaacgtgagg
     6421 ccgtatacgt gcgcgagcgt ggggacaccg gcgcctgacg atacggaccc gaagggaaga
     6481 gaaccggtcc gatggcaata gacgaatctt cttcctcttt cttctcgccg tggccggacc
     6541 ggttgcgcca ttcggccatc atcctgctcg ccgccggtct cgcgctcgcc gtgctcgtca
     6601 ccttcggcga attgtcgctg gtccgcgccg ccgcggtttt catctgcatc ggtgcggccg
     6661 cgctggtgcc gtggcggttg cacaacgcgg tcgcctcgcg tgaagatgtc cgcggggtca
     6721 acccggtcga agccgccgcg gtcagcgccg tggttgccgg catgccggat cccgcggtgc
     6781 tgctcgatcg cgccggccgc gtcatccacc tcaatgcggc ggcggcgcag ctcgcgccgg
     6841 cgctgcgcaa gaacgaactc gcccagttcg cgttgcgctc gcccgaaatc atcaccgcgc
     6901 tgcgcgaagc catcgccacc acggaaagcc ggcgcgcgac ctatctcgac cacgtgccgg
     6961 tcgatcgctg gatggaattg atcatcaccc cggtgccggt gccgacgctg ttcggcggca
     7021 tcgacaaatg catgctgatg acgtttcacg accagacgcc gctacgccgg gtcgaggaaa
     7081 tgcgtgccga cttcgtggcc aacgccagtc atgaattgcg cacgcccctg gccgcgctgt
     7141 cgggctttat cgacaccctg caggggccgg cccgggacga tgccaaggcg cgcgaacgct
     7201 ttctcggcat catgcacacc caggcgacgc ggatggcacg gctgatcgac gatttgttgt
     7261 cgctgtcgcg ggtcgaactg tcagcccatg tccgccccga tacggtggtg gatatcgtcc
     7321 cgatcatccg gcaggtggtg gacgggctgg agccgctggc gggcgaccgt caggtcgcga
     7381 tcgatatcca gctgccggac cggcccgtcg tgatcgcggg cgatcgcgag gagttgctgc
     7441 ggctgttcga aaatctgatc gagaacgcgc tgaaatacgg cgcatccggc ggcaagatca
     7501 ttgtctcgct cacctcggcg atgtcgggcg agggcttgcc ggagattcgc gtcatggtgc
     7561 gcgatttcgg ccccggcatc gcaccggaac atctgccgcg gctcaccgaa cggttctatc
     7621 gggtggatgt cggcgacagc cgcgcccagg gcggcacggg cttaggttta tcgctggtga
     7681 agcatattgt taaccggcac cgcggccggc tcttgatcga aagcgtgccg aaaaacggcg
     7741 ccacgttcac cgcctgtttt ccccagctgc gatcgcctgc agcggtataa atccaagcaa
     7801 tttcaatcgc ttgccaatgt catccaactg tcattaaagc ttcgtaaaag cggcattgac
     7861 cgcacctaaa cgggcggcgt gagcgcaatc gggcgcgtcc ggcgcttcgc tcaccgatgg
     7921 agaccagcaa tgaatttcgt gaaagctatc gtcgcggccg gcctggtcgc cgcttcgacg
     7981 tcggcattcg ccgccgacat tacaggcgcg ggcgcgacgt tcccgttccc gatctattcg
     8041 aagtgggccg acgcctacaa aaaggagacc ggcaacggtc tgaactacca gtcgatcggc
     8101 tcaggcgccg gcatcaagca gatcgaggcc aagaccgtga cgttcggcgc caccgacgcg
     8161 ccgctgaagg ccgagcagct cgagaaggat ggcctggtgc agtggccgat ggtgatgggc
     8221 gcgatcgtgc cggtcgtgaa tctggaaggt atcaagccgg gcgaactggt gctgtccggc
     8281 gaagtgctgg gtgacatcta tctcggcaaa atcaccaagt gggacgatcc ggcgatcgcc
     8341 aagctcaatg cgaaactgaa gctgccgact gacgccatta ccgtggtgcg ccgttccgac
     8401 ggctcgggaa ccaccttcaa tttcaccgac tacctgtcga agtcgaaccc ggactggaag
     8461 accaaggttg gctccgggac cgccgtcgaa tggccggcgg gcgtcggcgc caagggtaac
     8521 gaaggcgtgg ccggcaatat cggtcagacc aagaacgcga tcggttacgt cgagtatgcc
     8581 tatgccaagc agaacaagct gacctacgcg gc
//
LOCUS       DASWGM010000482         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_729043,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000482 DASWGM010000000
VERSION     DASWGM010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729043"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VGA15_13850"
     CDS             complement(<1..633)
                     /locus_tag="VGA15_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX9588812.1"
                     /translation="MHALRIARSKKLPRLAALLVAVASLGLAGCDENGGDPKGQIGAN
                     PVLPELQQYLIPPIRIAKVVGWGNDAPTVAQGLQVHAVATGFEHPRSLYVLPNGDVLV
                     VESNGPKAPISRPKDIITGWVQWFAGAKAKDANRITLLRDTNGDGKPELRTVFLDHLN
                     SPFGVALVGHDLYVANTDAIVRYPYQDGQTSITAEGTKLTDLPGGPIDHHW"
     gene            complement(639..1109)
                     /locus_tag="VGA15_13855"
     CDS             complement(639..1109)
                     /locus_tag="VGA15_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX9588813.1"
                     /translation="MKHATITETTFIGDTNLRLTAKPRGRPIHKMLVSFSAAYFAGAL
                     VTDLVYWQIPDVLWERFSIWLIAAGLIMAGLAAVAYVIDLAGGRQIDTPAWPRVVGYA
                     LAVLLSLMNAFVHSRDGYTAVVPTGLMLSGLVIVVLLLTAWVGMALVNRPRVGG"
     gene            complement(1457..2020)
                     /locus_tag="VGA15_13860"
     CDS             complement(1457..2020)
                     /locus_tag="VGA15_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9588814.1"
                     /translation="MRTRELFIAAFAVLAFVGTTRAQAQGAYCIGRVATPAEIAGWNI
                     DIDREGHNLPPGSGSVSHGHEVFDQQCAACHGAKGEGGLGDQLVGGQGTIATPKPVRT
                     VGSYWPYAPTLFDYIRRAMPQSAPQSLDDNDVYAVSAYILHLNGLLPADATLGAKTLA
                     AIKMPNRSMFVSDPRPDVKNPACMTGC"
     gene            complement(2007..3296)
                     /gene="soxC"
                     /locus_tag="VGA15_13865"
     CDS             complement(2007..3296)
                     /gene="soxC"
                     /locus_tag="VGA15_13865"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEX9588815.1"
                     /translation="MTKPTDIDKDIAATSRRRFLREGGALVGGAVIAGGLAGSEALGA
                     SESADNLPPNVPEWMKTPGDPMGSQLYGTPSPFEKGVIKNIPKNLSQYLSASGRTPLQ
                     DLDGIITPNGLFYERHHAGVPSIDPAQHRLMLHGLVDKPLIFTMDDIRRFPSESRIHF
                     LECSGNPGYKKPYGKTASDLVGLLSCAEWTGVSLKLVLQEAGLKQAAKWVVAEGADAA
                     ALTRSIPIEKCLEDAMLVYSQNGERLRPQQGSPLRLLLPGFEGNMSIKWLRRLRVAAE
                     PAYSREETSKYTDLMPDGSAREFTFYMEAKSIITRPSGGQRLSTPGFHEISGIAWSGH
                     GKIRGVDVSVDDGKSWQEAQLQEPVLTRALTRFRLPWQWNGRPAVIQSRATDETGYVQ
                     PTLSELLAVRGENYFYHNNAIWPWRIAASGEVTNANA"
     gene            complement(3312..>3563)
                     /locus_tag="VGA15_13870"
     CDS             complement(3312..>3563)
                     /locus_tag="VGA15_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX9588816.1"
                     /translation="GVEMDVLGLTYAEYSDDEREAIVHAHPRSELFKEDIIQAFYDGI
                     KHKPDTTFGNVKADVLADKDPAFRRGNFCSVIRGSAWSG"
BASE COUNT          685 a         1073 c         1058 g          747 t
ORIGIN      
        1 ccagtgatga tcgatcggcc cgccgggaag atcggtcagc ttggtgcctt cggctgtgat
       61 gctggtttgt ccatcctgat acggatagcg gacgatggca tcggtgttcg cgacgtaaag
      121 atcgtggcca accagggcga caccaaacgg cgagttgagg tggtccagaa acacggttct
      181 cagctcgggt ttgccatccc cgttggtatc gcggagcagc gtgatgcggt ttgcgtcctt
      241 tgccttggcg ccggcgaacc actgcaccca gccggtgatg atatccttgg gccggctgat
      301 cggtgctttc ggaccattgc tctcaaccac aagcacgtct ccattgggga ggacgtagag
      361 ggaccgcggg tgctcaaagc cggtcgccac cgcatggacc tgcaatccct gcgccaccgt
      421 cggagcatcg tttccccatc cgacgacttt cgcgatccga atcggcggta tcaaatattg
      481 ctgcaactcg ggcagcacgg gattggcgcc gatctgccct tttgggtcgc cgccattttc
      541 atcacagccg gcgagtccca ggctggcgac ggcgacaaga agggcggcga gacgcggtag
      601 cttcttcgaa cgtgcaattc tgagggcgtg catcatgatt atcctccaac gcgggggcga
      661 tttaccaagg ccatgccaac ccaggccgtc aacaggagaa cgacgatgac gagtccggaa
      721 agcatcaggc ccgtcggcac cacagcggtg taaccgtcgc ggctatgaac gaaggcgttc
      781 atcagcgaga gcaagacagc gagcgcatag ccaaccacgc gaggccaggc cggcgtatcg
      841 atttgtctgc cgcctgcaag gtcgatcacg taagcgacgg cggcaaggcc cgccatgatc
      901 agaccggcgg cgatcagcca gatcgagaac ctctcccaca gcacatcagg gatctgccaa
      961 taaacaagat cggtgaccag agcgccggcg aaataggcgg cggaaaatga caccagcatt
     1021 ttatggatcg gtcgtccacg aggcttcgcc gttaacctca gattggtatc gcctatgaaa
     1081 gtcgtttccg tgatcgtagc gtgcttcatg cttccatcct cataacgatc ccgcgcatct
     1141 gcgtttccaa agacaccggg ggttaatctc cgagccctaa caggcgacgt gaaaacttcg
     1201 ctgacaatct aacattggct cggcttcgct catgggtctt ctaaattcga gtttattttt
     1261 ttgagatcaa ctttagcgat tcgcgcgggc gccaagcagt aaattgcgcg gcgagagatt
     1321 tgttctccga tctggagctg cgggccccaa gtgaattcga atttagtgtt gcaacgcgga
     1381 gacgaacgag gggttaagga tttgtgaggt gcgatgggcg acagctagac ccagcgagcg
     1441 tctcttactt gtttcctcaa cagcccgtca tgcacgccgg atttttgacg tcgggacgcg
     1501 gatcgctgac gaacatgctc cggttcggca ttttgatcgc ggcaagcgtc ttggcgccga
     1561 gcgtcgcgtc cgccggcagc agtccgttca aatggaggat gtaggcggag accgcataga
     1621 cgtcgttatc gtcaagcgac tgcggcgcgc tctgcggcat ggcacggcgg atataatcga
     1681 acagcgtcgg ggcgtacggc caatagctgc cgacggtcct gaccggtttc ggcgttgcta
     1741 tcgtgccctg tccgccgacc aattggtccc caagtccacc ttcgcctttt gcgccatgac
     1801 aggccgcgca ttgctgatcg aatacctcat gcccatgact gacgctgccg cttcccggcg
     1861 gcaggttgtg gccctcgcga tcgatatcga tattccagcc cgcgatctcc gccggtgtcg
     1921 caacacgccc gatgcagtag gcgccttgcg cctgggcgcg agttgtgccc acaaaggcaa
     1981 ggacggcgaa cgctgcgatg aaaagctcac gcgttcgcat ttgtcacctc tccacttgcg
     2041 gcgatgcgcc acggccagat cgcgttgttg tggtagaagt agttctcgcc gcgaaccgcg
     2101 agaagttctg atagcgtcgg ctgcacgtaa ccggtttcat ccgttgcgcg gctctgaatc
     2161 acggcgggcc ggccgttcca ttgccagggc aatctaaagc gggtcagcgc ccgcgtcagc
     2221 actggttcct gcaactgcgc ttcctgccag ctcttgccgt catcgaccga gacatcgaca
     2281 ccccggatct tgccgtggcc gctccaggcg atgccgctga tctcatgaaa tccgggtgtg
     2341 ctcagccgct ggccgcccga gggccgcgta atgatggatt tggcttccat gtaaaatgtg
     2401 aactcgcgcg ccgagccgtc cggcatcagg tcggtatact tggaagtttc ctcgcgtgag
     2461 taagccggtt ctgctgcgac gcgcagacgg cgcagccatt ttatgctcat gttgccttca
     2521 aagcccggca acagcagccg cagcggagaa ccttgctgtg gacgcagccg ctcgccgttc
     2581 tggctgtaca ccagcatcgc atcttcgaga catttttcga tcggaatgct gcgtgtcaac
     2641 gccgcggcat cggcgccttc ggcaacgacc catttcgctg cctgcttcaa tcccgcttcc
     2701 tgcaaaacaa gcttcaggct cactccggtc cattcggcac agcttagcag tccaacgaga
     2761 tcggatgcgg tcttgccgta gggtttcttg tagccgggat tgccggagca ttcgagaaag
     2821 tgaatccgcg attccgaggg gaagcggcgg atgtcatcca ttgtgaagat cagcggcttg
     2881 tcgacgaggc catggagcat cagcctgtgc tgcgccggat cgatcgaggg gacgccggca
     2941 tgatggcgct catagaacaa tccgttcggc gtgattatgc cgtcgagatc ctgcagcggc
     3001 gtccgtccag acgcggatag atactgcgac agattctttg gaatattttt gataacaccc
     3061 ttctcgaacg gcgagggcgt cccgtaaagc tgacttccca tgggatcgcc gggcgtcttc
     3121 atccactccg gaacattggg cgggaggttg tcggcacttt cgcttgcccc cagcgcttcg
     3181 cttccggcga gaccgcccgc gatgacggcc ccacccacca atgcaccgcc ttcacgcaga
     3241 aaccgccggc gcgatgtcgc ggcaatatcc ttatcgatgt cggttggctt cgtcatcgta
     3301 cgcgcggccc gtcagccgct ccaggccgat ccgcgaatca cgctgcagaa attgccgcgc
     3361 ctgaaagccg ggtccttgtc cgcgagcacg tcggccttga cgttgccgaa tgtcgtgtcg
     3421 ggcttgtgtt tgatgccgtc gtagaacgcc tggatgatgt cttccttgaa tagctcggag
     3481 cgcggatgcg cgtgcacgat cgcttcgcgt tcgtcgtcgc tgtattcggc ataggtcagc
     3541 cccagcacgt ccatctcgac gcc
//
LOCUS       DASWGM010000483         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2272798,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000483 DASWGM010000000
VERSION     DASWGM010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272798"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..349)
                     /locus_tag="VGA15_13875"
     CDS             complement(95..349)
                     /locus_tag="VGA15_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588817.1"
                     /translation="MKMLPIAIGLAMAASLSACETAFSGPMSGQTAFVTDHATFASSP
                     AAGVVTTPESCPDPWPIDSLDRNNGNATPFSDRSFSYEGC"
     gene            383..1390
                     /locus_tag="VGA15_13880"
     CDS             383..1390
                     /locus_tag="VGA15_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9588818.1"
                     /translation="MALLLVAKRGEVPHGLRHKPDDEMLEMHSRHVYMCRMNWGVFDL
                     NLLIVFDAVMQERNVTRAGQKIGLSQPAMSHALNRLRYMLKDELFVRTPEGMVPTARA
                     EQLAAPLRRALSDMQLALEPETFVPQQADRRFTIAANNYAAVVLSPRIVMAVAAAAPS
                     AHLDVRPSGTLDIVDLLDRGELDLAVGDFAHPGERFAVAPLLEDPFVLAMRRGHPAGR
                     RKLTAEAFASLAHLEISSSGEDTSFIDRWLAGLTLKRSIMHRAPYLSAAGILSRSDMV
                     ATLSRRIAEAFIRSDPLQLRELPCPSPHVSIGMLWHRRFENQPAHRWLRGLIEATGKR
                     L"
     gene            complement(1470..2246)
                     /locus_tag="VGA15_13885"
     CDS             complement(1470..2246)
                     /locus_tag="VGA15_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9588819.1"
                     /translation="MIDRRTLSTMLIAGAATSLFGRSSKAQAQPAVSARNVVLVHGAY
                     ADGSCWSEVIGRLQLVGLRATAVQNPLTSLADDVAATRRILALQDGPTVLVGHSWAGT
                     VISEAGVDPKVSALVYVAARAPDAGEDYAALAAKFPTPPANAGLVKSGGFAQLSEEAF
                     LKDFAGGVDPAKARVLYAVQGRIADTLFASRTTAAAWKLKPSWYAVSKQDRTTSPELE
                     RFLANRMHAKTIEVDASHLSLITHPQEITDLILNAAGRSG"
     gene            complement(2489..>2707)
                     /locus_tag="VGA15_13890"
     CDS             complement(2489..>2707)
                     /locus_tag="VGA15_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588820.1"
                     /translation="GMAMVVDATGKATRAKISEDTAKAITAGAQPLSEAGIVLLYQGK
                     LYIVPDKRLGDGRMMSTMVMSSAPNANK"
BASE COUNT          481 a          870 c          844 g          512 t
ORIGIN      
        1 cagagacggc ggccgtcttg gccagcaaga acctcatcgc gatcttcctt tcggtttggc
       61 caatccgctc tccgtcgagc ggcggcgcga tcgatcagca cccctcgtag ctgaacgagc
      121 ggtctgaaaa tggcgtcgcg ttgccgttgt tgcggtcgag cgagtcgatc ggccaaggat
      181 cgggacagct ctcgggcgtg gtgacgacgc cggccgcggg ggacgacgcg aaagtcgcat
      241 ggtcggtgac gaacgccgtc tgccccgaca tcgggcccga aaacgcggtt tcgcaggcgg
      301 acaggctggc ggccatcgcc aggccgatcg cgataggaag catcttcatt gtggtaatcc
      361 ttctcgctgg aggtgaattg gtttggcgct gctgctggtt gccaagcgag gggaagtccc
      421 tcacgggctc agacataagc ccgacgatga aatgctggaa atgcattctc gtcacgtgta
      481 catgtgtaga atgaattggg gcgtcttcga tctcaatctg ctcatcgtct tcgatgcggt
      541 gatgcaggag cgcaacgtca cgcgcgcggg gcaaaaaatc ggcctgagcc agccggcgat
      601 gagtcacgcg ctcaaccggc tgcgctacat gctgaaagac gagcttttcg tacgcacgcc
      661 cgagggaatg gtgccgaccg cccgcgccga gcagctggcg gcgccgctgc ggcgtgcgct
      721 cagcgatatg cagctggctc tcgagcccga gaccttcgtt ccccagcaag cagaccggcg
      781 cttcacgatc gccgcgaaca attacgccgc tgtcgtcctg tcaccccgga tcgtcatggc
      841 ggtggcggcg gcggcgccat cggcccacct cgacgtccgc cccagcggca cgctcgacat
      901 cgtcgacctg ctcgaccgcg gcgagctcga cttggcggtc ggtgatttcg cccatcccgg
      961 cgagcgcttc gccgtggctc cgttgctcga ggaccctttc gtcctcgcca tgcggcgcgg
     1021 ccatcccgcc ggccgtcgca agctgacggc cgaggcgttc gcttctctgg cccatctgga
     1081 aatttcctcg agcggcgaag acaccagctt catcgaccgc tggctggccg ggctgaccct
     1141 gaagcggagc atcatgcatc gggcgcccta cctttcggcg gccggcatcc tgtcgcgctc
     1201 tgatatggtg gcaacgctca gccggcgcat cgccgaggcg ttcatccgca gcgatccgct
     1261 gcagctccgc gagctcccct gtccctcgcc gcacgtgtcg atcggcatgc tatggcatcg
     1321 gcgattcgag aatcagccgg ctcaccgctg gctgcgcggg ctcatcgagg cgacgggcaa
     1381 gcgtctttga gacgatcgca gccttcgcac cgagcgtaca aggctagcga gcctttgcgc
     1441 gacaacggct tggatttggt tggagccggt cagccgctac ggccggcggc gttcaagatg
     1501 aggtcagtaa tctcctgcgg gtgagtgatc agagacaagt gactggcgtc cacctcgatc
     1561 gtcttcgcat gcatacggtt tgcaaggaaa cgctccaact caggagacgt ggttctatct
     1621 tgcttcgata cggcatacca agaaggcttg agtttccacg ctgctgcggt cgtcctcgag
     1681 gcgaaaagcg tgtcggcgat ccgcccctga accgcgtaga gcacacgcgc cttggcggga
     1741 tcgacgccgc cggcgaagtc tttgaggaag gcctcctcgc tcagttgggc gaagccgccc
     1801 gacttgacca ggccggcatt tgccggtggc gtgggaaatt tggcagccag tgccgcatag
     1861 tcctcgccgg catccggcgc gcgagcagcg acgtagacaa gggcagagac tttgggatca
     1921 accccggcct cgctgatgac tgttcctgcc caggagtggc ctacgagcac cgtcggcccg
     1981 tcctgcaacg caaggatgcg gcgcgtggcc gcgacgtcgt ccgcaagcga ggtcagcggg
     2041 ttctgcaccg cagtcgccct caggccgaca agctgaagac gtccaatgac ttcggaccaa
     2101 caggaaccgt ccgcataggc accatggacc aggacgacat tgcgggcact gaccgcaggt
     2161 tgagcctgag ccttgcttga acggccgaaa agcgaggttg cagcaccagc tataagcatg
     2221 gtcgagagag tgcgtcgatc aatcatgtcg atacctccta ttttggggcg gtgccttttg
     2281 agctccaaac cgcgccgcct gccggcgcga catgccccca ctgatcgcac gaataacccg
     2341 gtcgcgtagg tcaaccgaca acgctccgct catgcatgct tcaccagcat gcagtttgaa
     2401 tcagaccttc cctggtttgg gaatcccgca ccgattcact cagttcgaaa aacgctctgg
     2461 gacgccagct tccgtcagcc gcacgttctc acttgttcgc gttcggtgcc gaggacatca
     2521 ccatggtgga catcatcctg ccgtctccga gccgtttgtc ggggacaatg tagagcttcc
     2581 cctggtacag caggacgata ccggcctcgc ttagcggctg cgcgccggcc gtgatcgctt
     2641 tggcggtatc ctcggagatt ttcgccctgg tcgccttgcc ggttgcatcg acgaccatgg
     2701 ccattcc
//
LOCUS       DASWGM010000484         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2272946,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000484 DASWGM010000000
VERSION     DASWGM010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272946"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..306)
                     /locus_tag="VGA15_13895"
     CDS             complement(64..306)
                     /locus_tag="VGA15_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX9588821.1"
                     /translation="MPTLDKVTVDLMVVLGTTSMPIHQVMRLSRGAIIELDATEADEV
                     KILANNLPIASGVVLVDRNRIAVEVKQMLPRLPGSR"
     gene            375..1100
                     /gene="lipB"
                     /locus_tag="VGA15_13900"
     CDS             375..1100
                     /gene="lipB"
                     /locus_tag="VGA15_13900"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX9588822.1"
                     /translation="MVNDRQSLDLTTFARPADDGAVEWQVSHAPVPYLEAVAAMEARV
                     AAIAAGEAPELVWLLEHPPLYTSGTSGKADDLLDARFPLFVTGRGGQLTYHGPGQRVA
                     YVMLDLKRRRPDVRAYVASLEEWIIRTLDAFDVHGERREDRVGVWVQRPDKGSGFEDK
                     VAALGVRLRRWVSFHGISINVAPDLTHFDAIVPCGVVDPRYGVTSLADLGHPATMADV
                     DIALRRAFEEVFGAVQTRLPEPI"
     gene            complement(1125..2021)
                     /locus_tag="VGA15_13905"
     CDS             complement(1125..2021)
                     /locus_tag="VGA15_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9588823.1"
                     /translation="MTAMLKPLLAICFGQPIKTRASYWLARLGPMVVLALLFGFGQTR
                     AQAPSVAVSGVDAALIEDIVIGSRVLADFGVLDGFGHVSARHPTNPNHFLMSRSLAPA
                     LVTADDIMEFDLDGNAVGNSGRSVFLERFIHSEIYKARPDVMSVVHTHSPGVVPFTVS
                     QVPLRPMYHNAAFLAAGAPVWDIRRDFGETDMLVRDPSLGKSLAQTLGDKSVVLMRGH
                     GDVTVGPAVKVAVFRAYYTDVNARLQSQAVALGGDVTFLTPGEGAKADAINLLVLDRI
                     WNLWKMRIAPSLGPSLGSSLGK"
     gene            complement(2127..3566)
                     /locus_tag="VGA15_13910"
     CDS             complement(2127..3566)
                     /locus_tag="VGA15_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX9588824.1"
                     /translation="MTSSFLWTLIAIQIAMGAFDTFYHHELTERLAWRPSQRHELQLH
                     SVRNALYALLFLTLGWLEVHGFWAMIMIAVLIVEVVVTLMDFVEEDLSRKLPASERIN
                     HTLLALNYGAILVLLLPILIEWAGQPPAIKSVFQGFWSILATVSAVGAGLGGARDFAA
                     SRRLARLNIATVSGLASELPGRQTILVTGATGFIGSRLVASLADAGHQVIALLRNPAK
                     AESLRPPITLITSLDQLPADAKIDAIVNLAGEPIGNGLWTEAKRRKILDSRIDMTGAL
                     VQLIARLERRPAVLVSGSAIGWYGLWQDQPLTESAKAHACFSHELCDAWEKAASRAAE
                     YGVRVVHVRIGLVVGTEGGLLTRMLTPFEFGLGGPMGSGQQWMSWIERDDLVRLIVHA
                     LAKSDISGPINATAPIPVRNMKFTEELGRRLHRPAVFRIPAALLHRLAGDFADELLLG
                     GQRVIPNKALSSGFVFRHETLRSAFEAIL"
     gene            complement(3563..4243)
                     /locus_tag="VGA15_13915"
     CDS             complement(3563..4243)
                     /locus_tag="VGA15_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEX9588825.1"
                     /translation="MVASQSKILKVPAPTASDESLDATLCDLRFRALLSDEDWGRLPL
                     ATWRRFSKRLADGKTAIYVGEVVEVTLNRVGWWLAQIARLIGGPLPTGGDTHVPSIVT
                     VTEDAATGGQVWTRIYGRRNGFPQVIHSSKRFSGPTGLEEYVGFGVSMALRMLVDGQA
                     LLFRSAGYFVQVGRLRLPLPAFLTPGALTVTHSDCGSGEFRFTLEIIHPRFGTLIRQS
                     AVFREATP"
     gene            complement(4265..>4376)
                     /locus_tag="VGA15_13920"
     CDS             complement(4265..>4376)
                     /locus_tag="VGA15_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEX9588826.1"
                     /translation="VDRWYAQMLTVPRPRLIALIKLGEKIVSFLPAGKSK"
BASE COUNT          901 a         1354 c         1332 g          789 t
ORIGIN      
        1 gatcaccggc gccgatgtaa caaatcaatg atggagggac aaggcctgcc cgcgtgacgg
       61 ttcttaccgg ctgcccggca gtcgcggcag catttgcttg acttcgaccg cgatacggtt
      121 gcgatcgaca agcacgacgc cgctggcgat cggcaggttg ttggcgagga tcttgacctc
      181 gtccgcttcg gtggcgtcga gttcgatgat ggcgccacgg ctcaggcgca tgacctgatg
      241 aatgggcatc gaggtggtac cgagcaccac catgagatcg accgtgactt tatcgagcgt
      301 tggcacttgg gaagcgaccc gtacagacga ttgcgaaatt gcgtaatttg agatcaccac
      361 gttatggtta gccaatggtt aatgaccgcc aaagcctcga tttgacgacc ttcgcgcgcc
      421 ccgccgacga cggcgcggtc gaatggcagg tttcccacgc gccggtgccc tatctggaag
      481 ccgtagcggc catggaggcc cgggttgcgg ccatcgcggc tggcgaagcc cccgaactgg
      541 tgtggctgct ggaacatccc ccgctctaca cttccggcac cagcggcaag gccgatgacc
      601 tgctcgacgc tcgttttcct ttgttcgtga ccggacgcgg cggacagctc acctatcacg
      661 gccccggcca gcgtgtggcc tatgtgatgc tcgacctgaa gcggcgccgg cccgatgtgc
      721 gggcctatgt ggcgagcctt gaggaatgga tcatccgcac cctggacgca ttcgacgtgc
      781 acggcgaaag gcgcgaggat cgggtcggtg tctgggtgca gcggccggac aaggggtctg
      841 ggttcgaaga caaggtcgca gcccttggcg tgcggctgcg acgctgggtg tcgttccacg
      901 gcatctccat caatgtggcg cccgacctga cgcacttcga cgccatcgtg ccctgcggcg
      961 tggtggaccc gcgctatggc gtcaccagtc tcgccgatct cggccatccc gcgaccatgg
     1021 ccgatgtcga tatcgcgctc cggcgggcgt ttgaagaggt gtttggcgcc gtgcaaacgc
     1081 gcttgccgga accgatttga ccatgctgcc ctcggtgcgg cgggctactt cccaaggctt
     1141 gaaccaaggc tcggaccaag gctgggagcg atgcgcatct tccacaggtt ccagatgcgg
     1201 tcgagcacta agaggttgat cgcgtcggct ttcgcgccct ccccgggcgt cagaaaggtg
     1261 acgtcgccac caagtgctac ggcttgcgac tgcagccggg cgttgacgtc ggtgtaataa
     1321 gcccggaaaa cggccacttt gacggctggc cccacggtca cgtcgccgtg gccgcgcatc
     1381 aagaccaccg acttgtcgcc gagcgtctgc gccagcgatt tgccaagcga gggatcgcgc
     1441 accagcatgt cggtctcgcc aaaatcccgt cgaatgtccc atacgggtgc gccggccgcc
     1501 aggaaggccg cgttgtgata catcggccgc aacggcacct ggctcaccgt gaatggaacg
     1561 acgccggggg aatgcgtatg aaccaccgac atcacgtccg gccgcgcctt gtagatttcg
     1621 ctatgaatga agcgttccag aaacaccgat cgaccgctgt tgcccacggc gtttccatcg
     1681 agatcgaact ccatgatgtc gtcggcggtg acgagcgccg gcgcgagcga ccgcgacatg
     1741 agaaagtggt tgggattggt cggatgacgc gcgctgacat ggccaaaacc gtcgagaacg
     1801 ccaaagtcgg cgaggacgcg gctgccaatc acaatatctt cgatcaaggc ggcatcgaca
     1861 cctgaaaccg cgacggacgg cgcttgggcg cgcgtctgcc caaaaccgaa caagagcgcc
     1921 aacaccacca tcgggccgag gcgcgcgagc caatagctcg cgcgtgtctt gatgggctgg
     1981 ccaaagcaga tcgccaataa cggtttcaac atcgcggtca tggtcatctc caattaaacc
     2041 gctgagcatc catggcttgt tgcatcagga aattatccga agcgcaaccc gtcgaacctg
     2101 cgagggtaag ggtgttagcg ctgcccctac agaatagcct cgaatgcact gcgcaaggtt
     2161 tcatgccgaa acacgaaccc gctgctcaac gccttgttcg ggatgacgcg ctgccctccc
     2221 agcagaagct catcggcgaa atcgccggcc aggcgatgga gcaatgctgc cggaatgcga
     2281 aataccgcag gtcgatgcag ccgccggccg agttcctcgg tgaatttcat gtttctgaca
     2341 gggatcgggg ccgtcgcgtt gatcgggccc gaaatgtcgg actttgcgag ggcatgaacg
     2401 atcagacgaa cgaggtcgtc gcgttcgatc caggacatcc actgctgtcc tgatcccatc
     2461 gggccgccca gaccaaactc gaatggcgtc agcattcgcg tgagaagccc tccttcggtc
     2521 ccgaccacca ggccgatcct gacatggacg accctgacgc cgtattccgc ggcgcggctc
     2581 gccgccttct cccaggcatc gcacagttcg tggctgaaac aggcatgtgc tttcgcggac
     2641 tccgtcaaag gctgatcctg ccaaagccca taccacccga tcgccgaacc gctgaccagc
     2701 acggctggcc ggcgttcgag gcgtgcaatc aattgaacga gcgcgccggt catgtcgatg
     2761 cgggaatcaa gaattttgcg gcgtttggcc tcggtccaca acccgttgcc gatcggctca
     2821 cccgccagat tcacgatcgc atcgattttg gcatcggcag gcagctgatc gaggctggtg
     2881 atcaaggtga tcggcgggcg caagctttcc gccttcgcgg gatttcggag cagcgcgatg
     2941 acctgatgac ccgcgtcggc gagacttgca acaagccggc tgccgatgaa gccggttgca
     3001 ccggtgacca ggatcgtctg ccgtcccggc aattcgctgg cgaggccgct tacagttgcg
     3061 atatttaacc gcgcaagacg cctggatgcc gcgaaatccc gcgccccgcc gaggccggcg
     3121 ccgaccgcgg acaccgtggc gaggatgctc cagaagcctt gaaacacgga cttgatggca
     3181 ggaggctggc cggcccattc gatcaagatc ggcagcagca gaacaaggat ggcgccgtaa
     3241 ttcagtgcca gcaaggtgtg gttgatgcgt tcgctcgcgg gaagtttgcg gctcaaatct
     3301 tcttcaacga aatccatcaa ggtcacgacg acttcgacga tcagtacggc gatcatgatc
     3361 atcgcccaga atccgtgaac ttccagccaa ccgagcgtca ggaaaagcag ggcgtagagc
     3421 gcattgcgca cgctgtggag ctggagttca tggcgctgcg aggggcgcca agccaaccgc
     3481 tccgtcagtt catggtgata gaacgtatcg aatgcgccca tcgcaatctg aattgcgatc
     3541 aaggtccaca gaaacgatga cgtcatggcg ttgcctcccg gaacacggcg gactgacgaa
     3601 taagggtgcc aaagcgggga tgaatgattt caagcgtgaa tcggaattcg ccgctgccgc
     3661 aatcggaatg ggtcaccgtc aacgcacccg gcgtgagaaa cgccggcaac ggcaacctca
     3721 gacgcccaac ctgcacaaag taacccgcac tgcggaacag cagggcctgg ccatcgacaa
     3781 gcatccgcag cgccatgctg acgccgaacc cgacatattc ctccagacct gtcggtcccg
     3841 agaaacgctt cgacgagtgg atgacttggg gaaatccgtt gcggcgcccg taaatgcgag
     3901 tccagacctg gccaccggtc gcagcatctt cggttaccgt gacaatgctt gggacatggg
     3961 tgtcaccgcc agtcggcaga gggcccccga tcaatcgcgc aatctgcgcc agccaccatc
     4021 cgacccggtt aagggtgacc tcgacgacct caccgacata aatggcggtc ttgccatccg
     4081 cgagacgctt cgagaaccgt cgccaggtag ccagcggcaa cctcccccag tcctcatcgg
     4141 acagcaaagc gcgaaatctg agatcacata gggtggcgtc gagactttcg tcggaagccg
     4201 ttggcgctgg aactttcagg attttgcttt gtgacgccac catgcactcc agcaatctca
     4261 aaccttattt ggatttcccg gccggcaaga agctgacgat cttttcgccg agcttgatca
     4321 gggcaatcaa ccttggccgc ggcacggtca gcatctgcgc gtaccagcga tcgacc
//
LOCUS       DASWGM010000485         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1847122,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000485 DASWGM010000000
VERSION     DASWGM010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847122"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1420)
                     /locus_tag="VGA15_13925"
     CDS             complement(221..1420)
                     /locus_tag="VGA15_13925"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588827.1"
                     /translation="MIKNSLTLIAALGAIAGLVVTSPAGAQERAVKIAGFGAKSGVVR
                     VFGINSEAALKAALEQINNAGGVTLGDGTKGKIALEYYDDRCNAEEGISVVRRIASTE
                     AVIAVGPTCSNVAESLFGILQKKVGDSSDSGLQFPIIADVAAKGGLAKISEWAFRNVP
                     SEFEMYKSLFAWVKTQYPDVKTIYGGVEKDFAHSNATFGVIKAQAEASGLQLLGQGEW
                     LLNDATFSTQVREMKRANPDVVAISAHPFTTCGVLKEMQRQGVKPKLLIGLTSSSSIE
                     TLQGCGAQAEGIVIPTSFAPVTPEAIKAAEQVAKFGGSADLHSAAAYEIAFILKNVIE
                     SQKVMGKPDTVAADREKMRAGLAALKETDGLLGKVGRTADREAIKPFLFVHAKDSAWK
                     VLYKPES"
     gene            complement(1952..2794)
                     /locus_tag="VGA15_13930"
     CDS             complement(1952..2794)
                     /locus_tag="VGA15_13930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9588828.1"
                     /translation="MNGLRIRTMRPDEISIAVNWAAAEGWNPGLADDACFAAADPEGF
                     FIGELDGAPAATVSCVNYGASFAVLGFYIVREDLRGRGYGLRIWNAAIAHAGPRVIGL
                     DGVVAQQQNYRKSGFELAYANVRYGGTVAAPNAPQAGVIALTDVPLAVVEAYDATVFP
                     APRTAFLRAWIGSLGHVGCALVRDGGLAGWGVIRPCRKGRKIGPLVADDRATAEVVLS
                     ALLASVGGGEIFLDVPSINRDAVALAQDLGLAPVFETARMYTGAVPRLRLERVFGVTT
                     FELG"
     gene            2945..>3649
                     /locus_tag="VGA15_13935"
     CDS             2945..>3649
                     /locus_tag="VGA15_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9588829.1"
                     /translation="MTGVRLKHYGWGREGEGMSAEERRFVLGLYRAKFATDEFDTIAV
                     PGLEDLSLREPRVVPPASLVPFCTTERYDRAAHTYGKSYPDYVKAMLGDYNCTPDAVA
                     YPRNEAEISAVMDWAGAVGASLTPFGGGSSVCGGVEPRVDGIKHKAAVTLDLRNLSKI
                     TEVDPVSRAASIEAGTYGPSLENQLKPHGFTLRHFPQSFEYSTLGGWIATRSGGHFAS
                     LHTHIDDFVESLRVVTP"
BASE COUNT          711 a         1156 c         1097 g          685 t
ORIGIN      
        1 caccagcgtg aagccgattc cgagcaggga ataggtcgcg ccgaacagaa cgccatcggc
       61 aaccgattgc agcgtgtcaa tgacgtcgag ataggacatg ccttggggat ccggaattcc
      121 gcgtcgaaca ccgggcctga acccggaaca catcaataga gcgtcgatgc ggcaggcgag
      181 gcaaacccgg ccaggaccgg ttcgcctcgc tttgcagcgg tcaggattcc ggcttgtaca
      241 ggaccttcca ggcgctgtcc ttggcgtgga cgaacaggaa cggcttgatc gcctcgcgat
      301 cggcggtacg gccgaccttg ccgagcaaac cgtcggtctc cttcaatgcc gcgagcccgg
      361 cgcgcatctt ctcgcgatcc gcggcgaccg tgtcgggctt acccatgacc ttctgggatt
      421 cgattacgtt cttcaggatg aacgcgatct cataagccgc ggcgctgtgc aaatcggcgc
      481 tgccgccgaa tttcgccact tgttcagccg ccttgatggc ttccggggtg accggcgcaa
      541 agctggtcgg gatcacgatg ccttcggcct gcgctccgca gccctgcagc gtttcgatcg
      601 acgatgacga cgtgaggccg atgagcagtt tcggcttgac gccctggcgc tgcatttcct
      661 tgagcacgcc gcatgtcgtg aacggatggg cggaaatcgc taccacgtcc gggttggcgc
      721 gcttcatctc gcgcacctgg gtcgagaacg tcgcgtcatt cagaagccac tcgccctggc
      781 ccagcaactg gaggccgctg gcttcagcct gcgccttgat gacgccgaag gttgcgttgg
      841 aatgagcgaa atccttttcc acgccgccgt agatcgtctt gacgtccggg tactgcgtct
      901 tcacccaagc aaacagcgac ttgtacatct cgaactcgct cggcacgttg cggaatgccc
      961 actccgaaat cttggcaagt ccacctttcg cagcgacgtc ggcgatgatc gggaattgaa
     1021 gccccgagtc tgagctgtcg ccgactttct tctgcagaat gccgaacagc gattcagcga
     1081 cattggagca agtcggcccg acagcaatga cggcttccgt ggaggcgatg cggcgcacga
     1141 cggaaatgcc ttcttcggca ttgcagcgat catcatagta ttcgagggcg atcttgccct
     1201 tggtgccgtc gccgagcgtg acgcctccgg cattgttgat ttgctcgagc gccgccttca
     1261 gcgccgcttc gctgttgatg ccgaagacgc gcacgacacc cgacttggcg ccgaagcctg
     1321 cgatcttgac cgcgcgctcc tgagcgcctg cgggtgaagt aacaactagt ccggcaatgg
     1381 cacccagagc cgcaattaga gtcagagaat ttttgatcat gacattttcc ctcccgatcg
     1441 gcgaccgcat cgtaggcggc gaaatctgca gttatccgga actcggcgcc cggttctaat
     1501 gcgagaatat cgttgtatag cgataccacg gatgatttcg gaaaacgatg gaattgagtg
     1561 tccgataccg ctgtccatac tgtcaagcca atggccgggc aggccggacc aaacggcgca
     1621 aatcacttcg cgtctcgccg gacgtccgct ttgcccgaac tggacattcc cgagaggtca
     1681 gcttcttgac ccgaaacggt cactggcacg gtcatcgcgc tggtgggtct gttccggcga
     1741 gtttccccgc gatgaatctg ccgatcagct cgccgactcc cccgaattgc tcatcgttgc
     1801 ttgccgacgg cgaacatcaa gctaatcgaa gacccgacga aaagcctgac ggaaaaatct
     1861 acaagaatag cgctccgtaa cgggtcctct cgtgccatcg ctcgatcaac tgatcgcctg
     1921 aggcgcaacg tcgccgttgc tcgcgggggc gctaccccag ctcaaaggtg gtgacgccga
     1981 acacccgctc cagccgcaac cgcgggaccg cgccggtata catgcgcgca gtctcgaaca
     2041 ctggcgcgag ccccaagtct tgcgccaagg cgaccgcgtc gcggttgatg ctcggaacgt
     2101 cgaggaagat ttcgccgccg cccacgctgg ccagcaaagc cgacagaacg acctcggcgg
     2161 tagcgcggtc gtcggccacc agcgggccga tcttgcggcc cttccggcat ggacggatca
     2221 cgccccagcc ggcaagcccg ccatcgcgca cgagcgcgca accgacatgc ccgagcgagc
     2281 cgatccaggc gcgcaggaac gcggtgcgcg gagccggaaa caccgtcgcg tcgtacgcct
     2341 ccacaaccgc caatgggacg tcggtcagcg cgatgacccc agcctgcggc gcattcgggg
     2401 ccgcgacggt gccgccgtaa cggacgttgg cataagcgag ctcgaacccg gactttctgt
     2461 agttttgctg ctgcgccacc acgccatcga gcccgatcac gcgtgggccg gcgtgcgcga
     2521 tggccgcatt ccatatgcgc agtccgtacc cgcgcccgcg taaatcctct cgcacgatgt
     2581 agaagccgag gacggcgaag ctcgcgccgt aattgacgca ggagaccgtc gcggccggag
     2641 cgccgtcgag ctcgccgatg aagaagcctt ccggatcggc cgcggcgaag caggcgtcgt
     2701 cggcgaggcc cgggttccag ccttcggccg ccgcccagtt cacggctatc gagatttcgt
     2761 ccggccgcat cgtccggatg cgcaggccgt tcatggcggg cgatcgtaca gatgggctca
     2821 tgaaaccgat attaccgtga gtgagtcgtt accggcaaac aatagaacgc tatggcgcgg
     2881 cgctgtggtt cagaatattt tcttcgatct aagaaactct cgaaacaaaa cacgaggaac
     2941 gctcatgact ggcgtacggc tgaaacatta cggctggggg cgcgaaggcg aaggcatgag
     3001 cgcagaggag cggaggtttg ttctcggcct ctaccgagca aaatttgcaa ctgacgaatt
     3061 cgacacgatc gctgttccgg gccttgaaga cctctccttg cgggaaccgc gtgtggtgcc
     3121 gccggcgtca ctcgtcccct tttgcacgac cgaacgctac gatcgcgccg cgcacaccta
     3181 cggcaagtcc tatccggatt acgtcaaagc aatgcttggc gattacaatt gtacacccga
     3241 cgctgtggcc tatcctcgca acgaggcgga gatttcggcc gtgatggact gggcgggcgc
     3301 tgtcggcgct tcgctgacgc cttttggagg cggctcgagc gtgtgcggcg gcgtcgagcc
     3361 gcgcgttgac ggcatcaagc ataaggctgc cgtcaccctc gatctgcgca atctctccaa
     3421 aatcaccgaa gtggatccgg tatcgcgcgc cgcatcgatt gaagccggca cctatggtcc
     3481 gtctctggaa aaccagctca agccgcatgg cttcacgttg cggcatttcc cgcagagttt
     3541 tgaatattcg acgcttggcg gctggattgc gacgcgatcg ggcggccatt tcgccagtct
     3601 tcatacccac atcgacgatt tcgtcgaaag cctgcgcgtc gtgacgcca
//
LOCUS       DASWGM010000486         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2668549,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000486 DASWGM010000000
VERSION     DASWGM010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2668549"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGA15_13940"
     CDS             <1..283
                     /locus_tag="VGA15_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX9588830.1"
                     /translation="AFKNNREDHIAVYGPDNHMRLTGKHETAAIGDFTYGIADRGASI
                     RVPHSFANNGYKGYLEDRRPNSQGDPYQIASQILKTIASVPTVAKSAAA"
     gene            482..1168
                     /locus_tag="VGA15_13945"
     CDS             482..1168
                     /locus_tag="VGA15_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9588831.1"
                     /translation="MENVIRLLSTLALMGAVRSLAGRYQAELGTRIDADFAPTLGLLD
                     RLRGGEGADVVILTREGLDELAGEGTVVADSRVDLARSYVGIAVKAGADHPDIATESA
                     LRATLLGARSVAYSRIGASGIFFAQLIERMGIASEINAKARIIPSGFTAERLVTGDAD
                     LAVQQISELKQVAGVEIVGPIPLHMQSPAVFSAGRLAASKRVVQADELLKYLASPEAA
                     TVLRASGLEP"
     gene            1219..2070
                     /locus_tag="VGA15_13950"
     CDS             1219..2070
                     /locus_tag="VGA15_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9588832.1"
                     /translation="MIRLQAFSFAAIGCIAVSAIIIVARPTLAHAQSADLVLCDRLAA
                     DPSDPDKPADVKGTSDVAAADVATAIKYCKVASASSRRALYQLGRAYAANRQLPEAMA
                     VWRKAADKGSTSAMVELGVAYGTGAGVARDEAQARKLFERAAEAGNPRGVTNLAALSG
                     SGGAPADPARARQLLAKAAETNAEAQYQLGLMMANGTGGAQDDAGARNLFEKAAAQNH
                     PGALERMGAFTQEGRGGPKDSGAAKAYYERAAALGDEDAKKALKRMECPYAIKDKRGN
                     FVTNLCF"
     gene            2472..3017
                     /gene="msrA"
                     /locus_tag="VGA15_13955"
     CDS             2472..3017
                     /gene="msrA"
                     /locus_tag="VGA15_13955"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX9588833.1"
                     /translation="MTVSTERAVLAGGCFWGVQDLLRRYPGVISTRVGYSGGEVPNAT
                     YRNHGNHAEAIEIIFDPKIIAYRKLLEFFFQVHDPSTLNRQGNDRGASYRSAIFYTSD
                     EQKRIAEDTIADVDASGLWPGKVVTEVAPVGPFWEAEPEHQDYLEKYPDGYTCHFIRP
                     NWTLPHRAEQAQSGTRGATAA"
     gene            3014..>3129
                     /locus_tag="VGA15_13960"
     CDS             3014..>3129
                     /locus_tag="VGA15_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012755690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9588834.1"
                     /translation="MTKGSDLLVAALENEGVQCIFAIPGEENLDVLESLRRS"
BASE COUNT          622 a          954 c          982 g          571 t
ORIGIN      
        1 agcgttcaag aacaaccgcg aagatcatat cgcggtctat ggcccggaca accacatgcg
       61 cttgaccggc aagcacgaga ccgctgcgat cggcgacttc acttacggca tcgcggaccg
      121 cggcgcatcg atccgcgtgc cgcacagctt cgccaataac ggctacaagg gctatctcga
      181 agaccgccgc ccgaactcgc aaggcgaccc ctaccagatc gcttcccaga tcctgaagac
      241 gatcgcgtcg gttccgaccg tcgccaagtc ggccgcggcc taagccttcg ccaccaagtc
      301 tgggtggaga cgtccaggcc gcaatccgat ccgccaaagt gtgaagcgct ttggcggatc
      361 attgcgtttg aggtgctcca ggaaaatgtt cttgacggat atttgtgcgt attctgtccg
      421 caaaaccggt aatcgctctc ggatcaagtc cgagggcatg cttttgcgga atgcgcgcta
      481 aatggaaaat gtcattcgcc tgctttcgac gctggcgttg atgggggcgg ttcgcagcct
      541 cgcgggaaga tatcaagcag agctggggac gcgcatcgat gccgatttcg cgccaacgct
      601 tgggctgctg gatcgcctcc gcggcggcga gggcgctgac gtcgtcattc tgactcgcga
      661 agggctcgac gaactcgcag gcgaaggaac agtggtcgcg gatagccgcg tcgatctggc
      721 gcgctcctac gtcggcatcg cggtgaaggc cggcgctgac catcccgaca ttgcaaccga
      781 gtccgcactg cgcgcgacac tgctcggcgc gcgttcggtg gcgtattcgc ggatcggcgc
      841 cagcggcatt ttcttcgcgc aactgatcga gcgaatgggt attgcgtccg agatcaatgc
      901 caaggcccgg atcattccgt cgggcttcac cgccgaacgc ctcgttactg gtgacgctga
      961 ccttgcggtg cagcagatca gcgagttgaa gcaggtcgcg ggcgtcgaaa tcgtcgggcc
     1021 gatcccgctt catatgcaaa gccccgcggt gttttcggcc gggcgtctgg cagcctcaaa
     1081 gcgggtggtt caagccgacg aacttttgaa gtatctcgcc tcgccggagg cggcaacggt
     1141 gctgcgcgcg tccgggctcg agccttgaat atcagggcag ttcgacgcta acctctcttg
     1201 gtcaaaacga gcgtcatgat gatacggctt caagcgtttt cgttcgccgc gataggttgc
     1261 atcgccgtct cggcgatcat cattgtagcc aggccaactt tggctcacgc ccaatcggcc
     1321 gatctcgtgc tgtgcgaccg tctcgccgcc gacccgtccg atccagacaa gccggctgac
     1381 gtcaaaggca cgtccgacgt tgcggccgcc gatgtcgcga ccgcgatcaa atattgcaag
     1441 gtagcttccg cctcgtcgcg gcgcgcgctg taccagcttg gccgcgccta cgccgccaac
     1501 cggcaattgc cggaagcgat ggctgtctgg cgcaaggcag ccgacaaagg ctctacctcg
     1561 gcgatggtcg aactcggcgt ggcctacggc acgggcgctg gcgtggccag ggacgaagcc
     1621 caggcgcgaa aactgttcga gcgcgccgcc gaagccggca atccacgcgg cgtcaccaat
     1681 ctcgccgcac tctccggcag cggcggcgcg ccggcggatc ccgcgcgagc aaggcagctg
     1741 ctggcgaaag ccgccgagac caatgccgaa gcgcagtatc agctcggttt gatgatggcg
     1801 aatggcaccg gcggggcgca ggacgatgcc ggcgcgcgga acctgttcga aaaagctgcc
     1861 gcgcaaaatc accccggcgc actggagcgg atgggcgcgt tcacccagga gggacgcggt
     1921 ggaccgaagg attccggcgc cgccaaggct tattacgaaa gggccgcggc actcggcgat
     1981 gaagacgcca agaaggcgct caagcgcatg gagtgccctt acgcgatcaa ggacaagcgc
     2041 ggaaatttcg tcaccaatct ttgtttctag tctagcgcgc cgtcaaaagt cactgaacga
     2101 gaggcctcca aagattgtca tgcccgcgaa agcgggcatc cagtaaacgc gccgaagcat
     2161 tgggatttct gcagcggcat ctccggccgt agcatgccgg cgccaagagg cgcgtccggg
     2221 aatggcagtc aggctgttat tgaacttcga taggcgcggc gccatctatc tcggcatcag
     2281 aagttctgcg caaaagcgaa cggatcgcgc gaacgcgcgc gccagcggga tgttttttct
     2341 ggaaggttgc gtgcgtaaat cttcgtgaag aggcctcgta tatttgcatt tttgcatcgt
     2401 tcggcctcat tgtatcccct tcgatggaag ctcggagaac gccccgcgag ggaaccttag
     2461 ggagaatgga catgacagtt tcgacggaac gcgcagtcct tgccggtggc tgcttctggg
     2521 gtgtgcagga tctgctgcgg cgatatcccg gcgtgatttc aaccagggtg ggctacagcg
     2581 gcggcgaggt gccgaacgcg acctatcgca atcacggcaa tcatgcggaa gccatcgaga
     2641 tcatctttga tcccaagatc atcgcttacc ggaaactgct ggaattcttt ttccaggtcc
     2701 acgatccctc gacgttgaac cgtcagggca acgacagggg cgcgagctac cggtcggcga
     2761 tcttctacac cagtgatgag cagaagcgga tcgccgaaga caccatcgcc gatgtcgatg
     2821 cctccggcct ctggccgggc aaggttgtca ccgaggtggc gcccgtcgga ccgttctggg
     2881 aggccgaacc cgagcatcag gattatctgg agaaatatcc tgatggttac acctgtcact
     2941 tcattcggcc gaactggacg ctgccgcacc gggcggagca agcgcaatca ggcacccgcg
     3001 gagctacggc agcgtgacaa agggctccga cctcctggtg gccgccctcg agaacgaggg
     3061 cgtccaatgc atcttcgcca ttcccggcga ggaaaacctc gacgtgctgg agtcgctgcg
     3121 gcgctcgaa
//
LOCUS       DASWGM010000487         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1719662,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000487 DASWGM010000000
VERSION     DASWGM010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719662"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..1431)
                     /locus_tag="VGA15_13965"
     CDS             complement(286..1431)
                     /locus_tag="VGA15_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX9588835.1"
                     /translation="MTTTGSSPFGAFACDSGSLKELLGDTALNCPCCNGRLTPEKLAR
                     LLDDVQKLAQGRAPANAGPAAQPSNGAGRPTGMIIDPHTHMISRTTDDYEAMAKAGVV
                     AVIEPAFWLGQPRTNVGSFIDYFSLISGFERFRAGQFGIRHYCTIGLNPKEANNEALA
                     EAVIDALPRFLVKEGVVAMGELGYDEQTPLEDKALRAQIELAKEFELPIMIHTPHRDK
                     RPGTLRTMDVLKEHGFDPARCVIDHNNEETIREVRDRGYWCAFSIYPNTKMSSERMVA
                     IVESYGPERLIVDSACDWGVSDPLAVAKTARLMAQRGVSADAVRQVVYSNALSVYGLN
                     TEMKEEHWLAPSAIDQRTLYEGNSVLRGGQTPRIEQPGRLTDELQIT"
     gene            complement(1428..1766)
                     /locus_tag="VGA15_13970"
     CDS             complement(1428..1766)
                     /locus_tag="VGA15_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9588836.1"
                     /translation="MTFPLQTGEVETPAVKNFKATGVNQCRGNDDAVADGKKLYTTNC
                     IVCHGADGTGKMGPTLVGKDIVYPEALTDPGMFSIIYGGAGAMQSFFRRGMQQDQMLR
                     IIAYVRTLEK"
     gene            2048..>2262
                     /locus_tag="VGA15_13975"
     CDS             2048..>2262
                     /locus_tag="VGA15_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588837.1"
                     /translation="MRQSAPTGLAFGPLSSVFDEPGLHARAQRIEVGEIDKRIRQNLA
                     ILADDPDAPGAFDDKDTPGAIIGCGHA"
BASE COUNT          446 a          685 c          674 g          457 t
ORIGIN      
        1 gctgcccaaa ccgatgttgg cattgtgaag gcaaccagcg agaaagctaa cgtgacagcg
       61 agcccgatca tcgttgtagg catatccgaa gcgtccctcg aacgcgtggt gaacatcttg
      121 ctaccatttt gctaccgaac cgggatattg ggcttatgca gggagggctg accccgacct
      181 ggatcggctt gccgctcgcc gcatccaccc aaaaggaata tccctgaatg gcaagcggct
      241 ggcagacgca gttgattgag aggcagcatc ttgatccggc gaggatcagg tgatttgcaa
      301 ttcgtcggtc aagcgtcctg gctgctcaat ccgcggcgtc tgcccgccac gcagcaccga
      361 gtttccttcg taaagtgttc gctgatcgat ggccgacggc gccagccaat gctcttcctt
      421 catttctgtg ttcaaaccat agacactaag ggcgttgcta tagacgacct ggcgaacggc
      481 atcggcgctg acgccgcgct gcgccatcag gcgcgcagtc ttggcaaccg ccagcgggtc
      541 cgacacgccc caatcgcagg cggaatcgac gatcaagcgc tctggcccat agctttcgac
      601 gatcgccacc atccgttcgc tgctcatctt ggtgttggga taaatggaaa acgcgcacca
      661 gtatccgcgg tcgcgaacct cgcggatggt ctcttcattg ttgtggtcga tgacacagcg
      721 cgccggatcg aaaccatgct ccttcagcac gtccatcgtc cgcaacgtgc ccggccgctt
      781 gtcgcgatgc ggcgtgtgaa tcatgatcgg aagctcgaat tccttggcga gttcgatctg
      841 cgcccttaac gccttgtcct cgagcggggt ctgctcgtca tagccgagtt cgcccatggc
      901 gacgacgcct tccttgacca gaaagcgcgg caacgcgtcg atcaccgctt ccgccaaggc
      961 ttcgttgttg gcttccttgg ggttgaggcc gatggtgcag taatgacgaa tgccgaactg
     1021 gccggcgcgg aaacgctcga acccggaaat cagcgaaaaa taatcgatga aactgccaac
     1081 attggtgcgc ggctgcccga gccagaacgc cggctcgatc accgcgacca cgccagcctt
     1141 ggccatcgct tcgtaatcgt ccgtggtgcg cgagatcata tgcgtatgcg gatcgatgat
     1201 catgccggtg gggcggccgg cgccgttgga tggttgcgcg gccggcccgg cgttggcggg
     1261 cgcgcgtcct tgcgcgagtt tctgcacgtc gtccaacagg cgcgccagct tttcgggcgt
     1321 caaccgcccg ttacagcagg ggcagttcag cgcggtatct ccgagcagtt cttttaagga
     1381 accggaatca catgcgaagg cgccaaaggg cgacgagccg gtcgtggtca tttttcgagt
     1441 gtccttacat aggcgataat cctgagcatc tggtcctgct gcatgccgcg gcggaagaac
     1501 gactgcatcg caccggcgcc gccatagatg atggagaaca tgcctgggtc ggtcagcgcc
     1561 tccggataga cgatgtcttt gccgaccagg gttgggccca tctttccggt gccatcagcg
     1621 ccatggcaaa caatacaatt cgtcgtataa agtttcttgc cgtccgcgac cgcatcatca
     1681 ttgccgcggc attggttgac gccggtggcc ttgaagtttt tcacggccgg cgtttcgacc
     1741 tccccggtct gcagcgggaa cgtcatgggc gaattgtcca gcggactcct gaagtccgat
     1801 aggtcaccgg cagtggccgg agacaggcct agcgtacccc caacgactgt caacgccaac
     1861 agcgatacgc ggggtgcgga aaagaaattc agtcggaatc ctgatctgca aagggatctt
     1921 aaacacgggc ctgaaaacgg catcgcccgc aaaatgtgcg ggcgatgcta gctgtccgct
     1981 tacaaaggct cagtttcctt tggttatcgc gacctccatg ccgactgcca ccttgtcggc
     2041 aggtacgatg cgccagagcg cgcccaccgg gttggcgttc gggcccttgt cgtcggtgtt
     2101 cgacgaaccc ggtctgcacg cccgagccca gcggattgaa gttggtgaga tcgataaacg
     2161 gatccggcag aaccttgcca tccttgccga cgatcctgac gcgcccggcg cgttcgacga
     2221 caaagatacg cccggagcca tcattggctg cggtcacgcc ga
//
LOCUS       DASWGM010000488         6781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1003951,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000488 DASWGM010000000
VERSION     DASWGM010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003951"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGA15_13980"
     CDS             <1..285
                     /locus_tag="VGA15_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9588838.1"
                     /translation="WEGNKNVGSGRMEILDTSEPSKIVIKLDFFTPFEGHNTAEFTML
                     PQGDATNLTWLMHGPASFMSKVMQVFINLDNMIGKDFEIGLADLKRLTEK"
     gene            323..739
                     /locus_tag="VGA15_13985"
     CDS             323..739
                     /locus_tag="VGA15_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9588839.1"
                     /translation="MQVNPYLYYNGNCEAAFKFYEKVLGAKVEMMLTHESAPESMPTP
                     PEWKKKIMHARISIDGEVIMGSDAPPGHFHQPQGFSVSLLIEDLADAERKFKALSEGG
                     SVNMAFAKTFFAKGFGMCVDQFGIPWMVFCPIPMES"
     gene            complement(744..1187)
                     /locus_tag="VGA15_13990"
     CDS             complement(744..1187)
                     /locus_tag="VGA15_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588840.1"
                     /translation="MMRTAITKAVFLAVLLCGLVVPPAQAEVRILASPGGQVGPFLDL
                     FERVRASGERVVIDGPCLSACTLVLSMVPNNRICVTRRAILGFHAARSIDARGHLYAE
                     PEASELVLEAYPSPVRGWILRRGGLTSRLLLLRGRELAAIYPSCR"
     gene            1543..2295
                     /gene="cobS"
                     /locus_tag="VGA15_13995"
     CDS             1543..2295
                     /gene="cobS"
                     /locus_tag="VGA15_13995"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HEX9588841.1"
                     /translation="MNDWLDDLRTAVAFLTRLPLPHPMGPMPANFVRAQRMFPLVGAA
                     IGGAVGLVCLGMRMIGVPDLAAAALALGASAILTGALHEDGLADVADGFGGGRDPAAK
                     LEIMRDSRLGTYGALILMVSFATKLSALAALPDAVVVQCLIAAHALARAVLPAMSMSL
                     AYARKDGLAANAGRPDLATAAIAAAFALVITLLSLPWFDALCAALVAAAAAVGMAWLA
                     QRQIGGQTGDVLGGAEQVAETAILVTLAAQLA"
     gene            2305..2871
                     /locus_tag="VGA15_14000"
     CDS             2305..2871
                     /locus_tag="VGA15_14000"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9588842.1"
                     /translation="MSVETNFWLIRHAPVDGPRGVIHGPDAPADVSDAAAFAALRARL
                     PKKAFAVCSPARRTWETAIALGLDPIREPDFREQDFGAWTGRRHDDLAAELGAAYQDF
                     WKSPASNRPPGGESFVDQISRARAGLAVLPPGDVVLIAHSGTIRAVLAIALDLAPDGA
                     LRFVIDPLSLTRIDRLESGWRVVAVNQR"
     gene            complement(2963..3259)
                     /locus_tag="VGA15_14005"
     CDS             complement(2963..3259)
                     /locus_tag="VGA15_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588843.1"
                     /translation="MSGLLINLIIQIISGAIGGNVAASTAKNIDLGTLGNTIAGAVGG
                     GVGGQILGMLIPLLANSASTPDIGSIIGQVAGGGVAGAVLTAIVGAIKNRTTAT"
     gene            complement(3554..4342)
                     /locus_tag="VGA15_14010"
     CDS             complement(3554..4342)
                     /locus_tag="VGA15_14010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9588844.1"
                     /translation="MTPSPQKVALVTGAARGIGLAVAKRFLAEGWRVALLDIEGELLW
                     KSVETLAAAGDTLGLHCDVSDARAVASAIAEIERRFGRLDALVNNAGVAVFAPLLETS
                     DEDWNRVLTVNLTGPFLCTKAAAPLMREHGGGAIVNITSISAVRASTLRSAYGTSKAG
                     LAHLTKQLAVELASLGIRVNGVAPGPVETAMAKAVHTPAIRADYHDAIPLNRYGLEEE
                     LAEAVFFLCSDRSSYITGQILAVDGGFDAAGIGLPTLRGDRRNG"
     gene            4548..4931
                     /locus_tag="VGA15_14015"
     CDS             4548..4931
                     /locus_tag="VGA15_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9588845.1"
                     /translation="MIVVDVRDILPEQWRLGVETRMLVSARNGAAQLCIFEQWIAPGA
                     GAPTHTHPVEEVLTVREGEAEMWMDERRVVVSAGQSLIVPAERQHGFRNSGNVTLHLH
                     AVLASPIFEATPEGATESVRRWEAV"
     gene            complement(5116..5622)
                     /locus_tag="VGA15_14020"
     CDS             complement(5116..5622)
                     /locus_tag="VGA15_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9588846.1"
                     /translation="MDQAQRSGCPINLTLEVVGDKWSLLIIRDMMFGNRRHFRELLTK
                     SEEGISSNILADRLKTLLEQGIITWSDDPSHRQKGIYSLTEQGIELLPILAQMSGWGF
                     KYLRVSEELGIRAKLLSEGGPKMWAEFMDELRETHLGVKRRKKTGPSVGERLQAAYER
                     VVARKGKK"
     gene            5733..6380
                     /locus_tag="VGA15_14025"
     CDS             5733..6380
                     /locus_tag="VGA15_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9588847.1"
                     /translation="MSLTLHFHPLASFCRKALIALYENDTPFTPNMVDLGSAAERAAL
                     LRLWPIGKFPVLCDDTREQTIPESSIIIEYLDRHYPGPTKFIPVDSDLAWQTRLRDRF
                     YDLYLHLPMQKIIGDRLRPEAKEDPHGVEEARVQLRTSYRMIDQQMAAGTWAMGEAFG
                     LADCAALPALFYGNMVEPFGESHTNVTVYFERLKARPSVARVLREAEPYFKMVPK"
     gene            6396..6779
                     /locus_tag="VGA15_14030"
     CDS             6396..6779
                     /locus_tag="VGA15_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9588848.1"
                     /translation="MPSASKPEIIRAIFAAYLSNDREVVEESLTDDFRFTSPYDDEID
                     KATYFERCWRNSDWIERHELEKIFVEGDEAFVTYKCVAKGGRSFRNTEFFTFEGDKVK
                     RIDVYFGATYENGAFVKQQQQEQQQ"
BASE COUNT         1306 a         2069 c         2127 g         1279 t
ORIGIN      
        1 tgggaaggca acaagaatgt cggctccggc cgcatggaaa tcctggacac ctcggagcct
       61 tcgaaaattg tcatcaagct cgattttttc acgccgttcg agggtcacaa caccgccgag
      121 ttcacaatgc tgccgcaggg cgatgcgaca aatctgacat ggctgatgca tggtccggcg
      181 tcgttcatgt cgaaggtgat gcaggtgttc atcaacctgg acaacatgat cggcaaggat
      241 tttgaaatcg gcctcgccga cctgaaacgg ctcaccgaga aataaccgca ggttcgaaac
      301 ttcaatcacc aaggagcaag cgatgcaggt caatccttat ctatactaca acggcaattg
      361 cgaagcggcg ttcaagttct atgagaaggt gcttggcgca aaggtcgaga tgatgctgac
      421 gcacgaaagc gcgccggagt ccatgccgac tccgccggag tggaagaaaa aaatcatgca
      481 tgccaggata tcgatcgatg gcgaggtgat catgggatcc gacgcacccc cgggccactt
      541 ccaccagccg caggggtttt cggtctcatt gctgatcgag gacctcgctg acgccgagcg
      601 caagttcaag gccctgtccg agggcggtag cgtcaacatg gcatttgcga agactttctt
      661 cgccaagggc ttcggcatgt gcgtcgatca attcggcatt ccctggatgg tgttctgtcc
      721 gatcccgatg gagagctgac gctctatcgg cacgagggat agatcgccgc gagttctcgc
      781 ccgcgcaaca acaacagccg cgacgtcaat ccgccgcggc ggaggatcca gccccgcact
      841 ggggaggggt aggcctccag cacaagttcg gacgcttccg gttcggcgta cagatgtccg
      901 cgcgcgtcga tcgaccgcgc ggcgtgaaat cccaggatcg ctcgacgcgt cacgcagatg
      961 cggttgttgg gcaccatgct cagcaccagc gtgcaggccg acaggcacgg cccgtcgatc
     1021 accacgcgtt cgcccgaggc acgcacacgc tcgaacagat cgagaaacgg gccgacctgt
     1081 ccgcccggac tggccaggat ccgaacctcg gcctgcgccg gtggcacgac cagcccgcac
     1141 agcagcactg cgagaaaaac cgctttcgtg atcgccgttc gcatcatgag cggctccttc
     1201 gctcaggttg atcaaaatga aatagtcagt ggcaggacgc gcgacaaggc tccggtaccc
     1261 gtcggcgctg gaaacgccag cctcggataa tgtagccatg ccagtgtggc aggaagcgcc
     1321 gcggaacctt gccgtgatgg ccggcgaata cggcgacgga gactgcgaga ctccccgacg
     1381 gtcttgtcaa tgtctggcgg cggaccgtgg cagcgtcggc cgcgcacgcg tcgccaagcg
     1441 gcagaccgcg acagaatgca cgaatatcgt ctaacctggt ggccgcatgc gattctctgg
     1501 tcgggcgcgg cgcgaacaag ataattccat gggttctgaa tcatgaatga ctggctcgac
     1561 gatctcagaa ccgcggtcgc gttcctgacc cggctgccgt tgccgcatcc gatggggccg
     1621 atgcctgcga attttgtccg cgcgcagcgg atgtttccgc tcgtcggcgc cgcgatcggc
     1681 ggtgctgtgg gactcgtctg tctcggcatg cgcatgattg gggtgcccga tctcgccgcg
     1741 gcggcactgg ctctgggcgc gagcgcgatc ctgaccggcg cgctgcacga ggacggtctc
     1801 gccgatgtcg ccgacggatt cggcggcggc cgtgatcccg cggcaaaact cgagatcatg
     1861 cgcgacagcc ggctcggcac ttatggggcg ctgatcctga tggtgagttt tgcgacaaag
     1921 ctttccgcgc tcgccgcgct tccggacgcg gtggtcgtgc aatgcctgat cgccgcgcat
     1981 gcgctggcgc gcgctgtgtt gccggcgatg tcgatgagcc tggcttacgc gcgcaaggac
     2041 ggacttgccg ccaatgctgg caggccagat ctggcgacgg cggcaattgc cgctgcattt
     2101 gcgcttgtta ttaccttgct gtcgctgccg tggttcgacg cgctgtgcgc ggcactggtg
     2161 gcggccgctg ccgcggtcgg catggcgtgg ctggcgcaac ggcaaatcgg cggccagacc
     2221 ggcgacgtgc ttgggggcgc cgagcaggtc gccgagaccg cgattctcgt gacgctcgca
     2281 gcacagctgg cataagcttc atcgatgagc gtcgagacaa atttctggct gatccggcac
     2341 gccccggtcg atggaccgcg cggcgtcatc catggtccgg acgcgccggc ggatgtcagc
     2401 gatgctgcag ccttcgccgc gctcagggcg agattgccga aaaaggcttt cgccgtttgc
     2461 agtccggcgc gccggacgtg ggaaaccgct attgccctcg ggctcgatcc gatcagggag
     2521 cctgacttcc gcgaacagga ttttggcgcc tggaccggac ggcggcacga cgatctcgcc
     2581 gcggagctcg gcgccgccta ccaggacttt tggaaatcgc cggcgagcaa ccggccgccc
     2641 ggcggtgaaa gttttgtcga tcagatttcc cgggcgcgcg caggcctcgc cgtactgccc
     2701 ccgggcgacg tcgtcctcat cgcgcattcc ggcaccatcc gcgcggtgct cgccatcgcg
     2761 ctcgatcttg cacccgatgg cgcgttgcgt tttgtgatcg acccgctgtc cttgacgcgg
     2821 atcgatcgcc tcgagagcgg ctggcgggtg gtcgcggtca accagcgttg aatggggcat
     2881 gggtccatag cgcaccacga tcgttggcgc gatcaagcga ggcacgcatt cgtgccccgc
     2941 tccgttcatg atcgcagaac ggtcaagtcg ccgtcgtcct gttcttgatg gcaccgacga
     3001 tcgccgtgag aacggcaccc gcgacgccgc caccggccac ctggccgatg atgctgccga
     3061 tatccggcgt cgaggccgaa ttggcgagca gcggaatcag catgccgagg atctgtccgc
     3121 ctacgccgcc acccaccgca cctgcgatgg tgtttccaag cgtgccgaga tcgatattct
     3181 tagctgtgct cgcggcgaca tttccgccta tggcaccgct gatgatttgt atgatcagat
     3241 taatgagcaa tcccgacatg acgcgatact ccccggtccg ggttcagttt cagcttcatg
     3301 cagggcgccg gaccaccgct gcacggtaca acttaagcgt ttgaaccggg aaggtcatgc
     3361 tgcgccagcg aaggattgga aagaaaattg tagatgcttc ggcaatttca gccgtgtgaa
     3421 acgaggaatt gtagatgaat gccatcggtc atcgggataa tcccccggga agcctggcgt
     3481 tcgggcgtgg aaacccgcat gcaggtttcg gccggccatg gtgcggtgcg agcggggaga
     3541 gcgagaagag atgctagccg tttcgtctgt cgccgcgcag cgtcggcaat ccgatgccgg
     3601 cggcatcaaa accgccatcg acggcgagaa tctgtccggt gatgtagctg gagcggtcgc
     3661 tgcacaaaaa gaacaccgcc tccgccagct cttcttcgag accgtaacgg ttgagcggga
     3721 tcgcgtcgtg atagtcggcg cggatcgccg gcgtgtggac ggctttcgcc atcgcggtct
     3781 cgaccgggcc gggtgcgacg ccgttgacgc gaatgccgag tgaggccagt tccacggcga
     3841 gttgttttgt caaatgcgcc agcccggcct tgctggtccc gtaggccgag cgcagtgtcg
     3901 aggcgcgcac cgccgagatc gaggtaatgt tgacgatcgc gccgccaccg tgctcgcgca
     3961 tcagcggcgc ggccgccttg gtgcacagga acggtccggt gagattgacg gtcagcacgc
     4021 gattccagtc ctcgtcggat gtttccagca gcggcgcgaa caccgcgaca ccggcattgt
     4081 tgaccagcgc gtcgagccgc ccaaaacgcc gttcgatctc agcgatcgcg cttgcgaccg
     4141 ccctggcatc ggagacgtcg caatgcagtc ccagcgtatc gccggcggcg gccagcgtct
     4201 cgacgctctt ccataacaac tcgccctcga tgtcgagcag cgccacgcgc cagccctcgg
     4261 cgagaaaccg ttttgcaacc gcaagcccaa tgccccgcgc ggcgccggtt accagcgcga
     4321 ctttttgtgg agagggggtc atgagtggct ccgtctgcgg ttgatcgatc atgcactcct
     4381 atagaccatg cgttgcatcg tgtcccggac gcggtgcggc atgatctcta ccgcttgcgc
     4441 gtcatgcggc ttccggggca ggcttgaaac ggcaagccgt gtgcaagtct tggcgaatga
     4501 caagtcttgg cgaatgacaa gtcttggcga atgaaaggac cagtccgatg atcgtggtcg
     4561 atgtcaggga tatactgccg gagcaatggc ggctgggcgt ggaaacccgc atgctggtct
     4621 cggcccgcaa cggcgcggcg caattatgta tcttcgaaca atggatcgcg cccggcgccg
     4681 gcgccccgac ccatacgcat ccggtcgagg aagtgctgac cgtgcgcgag ggtgaagccg
     4741 aaatgtggat ggacgagcgg cgcgtcgtcg tctcggccgg ccagtcgctg atcgtgccgg
     4801 cagagcgtca acacggcttc cgcaactcgg gcaacgtcac gcttcacctc cacgcggtgc
     4861 tggcctcgcc gattttcgaa gcgacgccgg agggtgccac ggagagcgtg cggcggtggg
     4921 aggcggtgtg agcgccatca tcctgggccg tcatcctgag gtgcgagctc ttgcgagcct
     4981 cgaaggatga gacgttcagc ttggcttcgc gcggcatatg cactcatcgt agtccatcct
     5041 tcgaggctcg ccgaagacgg ctcgcacctc aggatgacgg aaaccgcgtt tcggtcaacc
     5101 caagacaagt tggggtcact tcttcccctt cctcgccacc accctctcat acgccgcctg
     5161 caaccgttca ccgacgctcg gccccgtttt cttgcgccgc ttcacgccca gatgcgtctc
     5221 gcgcaattcg tccatgaatt cagcccacat tttcggaccg ccttcgctga gcagcttggc
     5281 gcggatgccg agttcttcgc tgacgcggag atatttgaat ccccagcccg acatctgcgc
     5341 cagaatcggc aacagttcga tgccctgttc ggtcagcgaa tagattcctt tctgcctgtg
     5401 gctggggtcg tcggaccatg tgatgatgcc ctgctcgagc agcgtcttca gccggtcggc
     5461 gaggatgttg gaggaaatgc cttcctcgga tttggtcaac agctcgcgaa aatgccgacg
     5521 attgccgaac atcatgtcgc ggatgatcaa gaggctccat ttgtcgccga ccacttcgag
     5581 cgtgagattg atcgggcagc cggagcgttg tgcctggtcc ataagcgatg tccaaacatg
     5641 cggcgaaaac gccacgaaga accgcttgca atatcgcatc agttatccca aaatacaacc
     5701 agttgtaaaa cgcaatcagt taaggaggcc acatgtcgct cacgctgcat ttccacccct
     5761 tggcgtcgtt ttgccggaag gcgctgatcg ccttgtacga gaacgatacg ccgttcacgc
     5821 cgaacatggt cgatctcggc agcgcggccg agcgcgccgc gctgttaaga ctgtggccga
     5881 ttggcaaatt tccggtgctg tgcgacgaca cgcgggagca gactattccg gagtcgagca
     5941 tcatcatcga atatcttgac cggcactatc cgggcccgac gaaattcatc cccgtggact
     6001 ctgacctcgc ctggcagacg cggctgcgcg accggttcta cgatctctat ttgcatctgc
     6061 cgatgcagaa gatcatcggc gacaggcttc gccccgaggc aaaagaggat ccgcacggcg
     6121 tggaagaggc gagggtccag ctgcggacgt cctatcgcat gatcgaccag cagatggcag
     6181 ccggcacctg ggcgatgggg gaagcgttcg gcctcgccga ttgcgccgcg ttgcccgctt
     6241 tgttctatgg caacatggtc gagccgttcg gcgagagcca cacaaacgtc actgtctatt
     6301 tcgaacgcct gaaggcgcgc ccctcggtcg cacgcgtgct cagggaggcc gagccctact
     6361 tcaagatggt cccgaagtga agtaaggaga cgaaaatgcc ttcagcaagc aaacccgaaa
     6421 tcatccgcgc gatctttgcc gcctacctgt cgaacgatcg cgaggtcgtc gaggagtcgc
     6481 tcaccgatga cttccgtttt acaagcccgt atgacgacga gatcgacaag gcgacctatt
     6541 tcgagcggtg ctggcgcaac agcgactgga tcgagcggca cgaactggaa aagatcttcg
     6601 tcgaaggcga tgaggccttt gtgacctaca aatgcgtggc caaaggcggc aggagtttcc
     6661 gcaatacgga gttttttacg ttcgaaggcg acaaggtcaa acgcatcgac gtgtatttcg
     6721 gcgcgaccta cgagaatggc gcgttcgtga aacagcagca acaggaacag cagcaataaa
     6781 a
//
LOCUS       DASWGM010000489         9666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2412812,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000489 DASWGM010000000
VERSION     DASWGM010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412812"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VGA15_14035"
     CDS             <1..480
                     /locus_tag="VGA15_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX9588849.1"
                     /translation="RNGIAGAKMSEHGRANALDVHDLKLANGQTISFTDRTVSREVRE
                     NVLHSMCARFTTVLGPGSDGYHEDHIHLDLMERHNNYKICQWNVWDPLPQIAPLLPAE
                     RPEEAPPRELAAKSDAAKSDAAKPDAAKADAAKSDASEPATDATKAPTKKRRQNRRF"
     gene            complement(586..1263)
                     /locus_tag="VGA15_14040"
     CDS             complement(586..1263)
                     /locus_tag="VGA15_14040"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9588850.1"
                     /translation="MSSLSVKLGILAAGLMLSGCMQATTFEATNQTVFKPRDKELLAK
                     VSYVKTPVAEPFRRAIVDYHRKEAPGSIVVDSDNHYLYLVQDGGKAIRYGITVGEEAM
                     AWSGIAKIGSMTEWPPWHPTPGEISRLGVPKFVAPGPDNPMGSRALYLYSGGKDTLFR
                     IHGTNQPEYIGASISSGCIRMTNEDVIDLYNRVKMGTIVVVLEPKHGDSPFNSQMALQ
                     GGGGSFQ"
     gene            1602..2582
                     /locus_tag="VGA15_14045"
     CDS             1602..2582
                     /locus_tag="VGA15_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX9588851.1"
                     /translation="MLSMFVPSEASLKKVVCADLAALPESAVWIDLVKPTPAEDHAVE
                     RLAGIAVPTREDMQEIEISSRLYIENGARYMTATLMCAADTQNPRTTAVTFILAAHRL
                     VTVRYDEPKPFMLVENKLARACAPGVTGEMVLMELLDAVIDRNADILERAGSDMDNIS
                     HDIFEPEGAARTGHAKRYSNILIAIGRKGDLTSKVRESLVSIGRVVTFVAAGTEGVKW
                     SKDMREQLKTMQRDVTSLTDHASYLSNKITFVLDAMLGVVNLEQNNIIKLFSVMAVVL
                     MPPTLIASIYGMNFKSMPELEWTHGYPYALVAMLLAAVGPYLFFTWKKWL"
     gene            2711..2905
                     /locus_tag="VGA15_14050"
     CDS             2711..2905
                     /locus_tag="VGA15_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX9588852.1"
                     /translation="MKVKDAIRLLEKEGWFLVATRGSHRQYKHPVRPGRVTVAGKPSD
                     DLAPGTLNSILKQSGLKEQH"
     gene            2905..3111
                     /locus_tag="VGA15_14055"
     CDS             2905..3111
                     /locus_tag="VGA15_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX9588853.1"
                     /translation="MRYAIVIEKADANYSAYVPDLPGCIATGDTVAAVESEIRDAIRF
                     HIEGLQADGIVVPEPTSIAEYIET"
     gene            3355..3579
                     /locus_tag="VGA15_14060"
     CDS             3355..3579
                     /locus_tag="VGA15_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588854.1"
                     /translation="MVEKVITSSENVVDFARYQVDRNAGKARAISARACRHCGAALSE
                     GESEDECSSAFNIDNARLRWQAAQILRGLI"
     gene            complement(3763..4515)
                     /locus_tag="VGA15_14065"
     CDS             complement(3763..4515)
                     /locus_tag="VGA15_14065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9588855.1"
                     /translation="MPNSTNAPRRTLLLTGASRGIGHATVIRFSSAGWRVITCSRHPF
                     PEECPWGAGPEDHIQVDLADHADTTRAISEIRERLEGGDLHALVNNAAISPKTEGGGR
                     LGSIDTDIETWSHVFRVNFFAPIMMARGLIEELKAAKGSVVNVTSIAGSRVHPFAGAA
                     YATSKAALASLTREMASDFGRIGVRVNSIAPGEIDTSILSPGTERIVEQQIPLHRLGT
                     PDEVAKIIYVLCTETSSYVNGAEIHINGGQHV"
     gene            complement(4539..5180)
                     /gene="pdxH"
                     /locus_tag="VGA15_14070"
     CDS             complement(4539..5180)
                     /gene="pdxH"
                     /locus_tag="VGA15_14070"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX9588856.1"
                     /translation="MTDTTSIKHPTPLTSGDFTAAEEPFALFGEWFAEAVKAEPNDPN
                     AMSLATVDADGLPDVRMVLMKGYDASGFVFYSHIASQKGRELATNPKAALLFHWKSLR
                     RQIRIRGNVSPVTDAEADAYFATRPKQAQIGAWASKQSQPLESRFAFEQAIAKIAARH
                     ILGEVPRPPGWSGWRIAPLRLEFWHDRPFRLHDRIEFRRAKPDQPWSKTRLYP"
     gene            5303..5710
                     /locus_tag="VGA15_14075"
     CDS             5303..5710
                     /locus_tag="VGA15_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX9588857.1"
                     /translation="MILILIGAGSGGCSLSRTDGAFARMEDHGVTGSVGLAQAGGPMP
                     TESDLALARNAASDVLTKGTKDSSQPWENPETGARGSVTPLAEAYASDGRTCRDFLAS
                     YVNGRSESWLQGAACQSEQGRWEIHTLKPWRRG"
     gene            5818..6783
                     /locus_tag="VGA15_14080"
     CDS             5818..6783
                     /locus_tag="VGA15_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX9588858.1"
                     /translation="MRDPYEVLGVPRGASAAAIKSAYRKLAKKHHPDANKNDPKSAAR
                     FSELNSANEIIGDEEKRKQFDRGEIDADGKPRFQGFPGGDPRGRGASPGGGFETHTFR
                     TGQGGFSGGAGFEDILNSMFGGAAARGARPGAGGSFEFDPGGIAVDLDLNVAMTVSLE
                     EAVHGVTKRVRLPTGKELDVKIPAGVVAGQQIRLKGQGETAPGHRPGDLLITVGIAPH
                     AIFKVDGSDLRVDLPITLYEAVLGGKVRVPTLGSAVELSIPKNTSSGRTFRLKGKGLP
                     KATGMTGDLFVTTRIMLPDGNDAELEALMQKWRDGHPYNPRSDMT"
     gene            complement(7030..7800)
                     /locus_tag="VGA15_14085"
     CDS             complement(7030..7800)
                     /locus_tag="VGA15_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX9588859.1"
                     /translation="MTDPQISITEDELHAYVDNELPAERRGDVEAWLSAHPDDAERVQ
                     SWRAMAEALHARYDQVADEAVPKRLEIERLERQPRRWLYGAIAATLLAFVGGGGVGWL
                     ARGAAASPSAFQKLTVDALDAYRLYVVEVRHPVEVPGSERTHLQQWLTKRCGWAVHAP
                     ELDATGLKLVGGRLLPGPTGPASFLMYESVSGERFTLYTSRAKTGTAQMRYTAAENSG
                     AMYWSEDGVGYVLSGPVDKDRLNQVARLVYDQTEKPRG"
     gene            complement(8078..8566)
                     /locus_tag="VGA15_14090"
     CDS             complement(8078..8566)
                     /locus_tag="VGA15_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9588860.1"
                     /translation="MTAFRQSIEAMIPALRRYARALARDADIADDLVQDTLVRALRSE
                     RLFLGGDVRSWLYTILTNLNKNRRRSLARRPQFMPLLDNNPDASGTEAEGRDIARALA
                     TLVEEQRAVLLLVMLEGLSYREVADIQGVPIGTVMSRLARARAHVKASLEGERVALRR
                     VK"
     gene            8764..9594
                     /gene="fabI"
                     /locus_tag="VGA15_14095"
     CDS             8764..9594
                     /gene="fabI"
                     /locus_tag="VGA15_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX9588861.1"
                     /translation="MSQSSGLMQGKRGVILGVANSRSIAWGIAKACHAAGAEIALTWQ
                     GDALKKRVEPLAKELGGILLGHCDVTDPATIDAVFDVLREKWGKIDFVVHAIAFSDKD
                     QLDGRYLETTADNFSKTMLISCYSLTAIAQRAEKLLIDGGSILTLTYYGAEKWMPHYN
                     VMGVAKAALEASVRYLAADLGEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAP
                     MRRTVTTEEVGDSAMYLLSDLSRGVTGEVHHVDSGYHVVGMKRPDAPDISLSNSLSKE
                     "
BASE COUNT         1839 a         2986 c         3034 g         1807 t
ORIGIN      
        1 cgcaacggca tcgccggcgc caaaatgtcc gagcacggcc gcgccaatgc gctcgacgtt
       61 cacgacctga aactcgccaa cggccagacg atctcgttca ccgatcgcac ggtgtcgcgc
      121 gaggtgcgcg aaaacgtgct gcattcgatg tgcgcgcggt ttaccaccgt gctggggccg
      181 ggttcggacg gctaccacga ggaccacatc catctcgatc tgatggaacg gcacaacaac
      241 tacaagatct gccagtggaa cgtgtgggac ccgctgccgc agatcgcgcc gctgctgccg
      301 gccgagcggc ctgaggaagc gccgccgcgc gagctcgccg cgaaatccga cgcggccaaa
      361 tctgacgccg cgaaaccgga tgcagctaag gcagacgcgg cgaaatcgga tgcttctgag
      421 ccggccacag atgcgaccaa ggccccaaca aaaaagcgcc ggcaaaaccg gcgcttttga
      481 tctcgtcgtg acgacttcgt catcgaccgt cgtgacgact tcgtcatcga ccgtcgtgaa
      541 gtctacgtca tcgaccgttc tgatcgacgc tcgcgagagc tgcggctatt ggaagctgcc
      601 gccgccgccc tgcagcgcca tctgcgaatt gaacggcgag tcgccgtgct tcggctccag
      661 caccaccacg atggtgccca tcttcacccg gttgtacagg tcgatcacgt cctcgttggt
      721 catccggatg cagccggagg agatcgacgc gccgatgtat tccggctggt tggtgccgtg
      781 aatccggaac aaggtatcct tgccgccgga gtacagatac agcgcgcgcg aacccatcgg
      841 attgtccggt ccgggagcga cgaacttcgg cacgccgagc cgcgagattt cgccgggcgt
      901 cggatgccaa ggtggccact cggtcatgct gccgatcttg gcgataccgg accaggccat
      961 cgcttcctcg ccgacggtga tgccataacg gatcgccttg ccgccgtcct gcaccagata
     1021 aaggtaatga ttgtcggaat cgaccacgat cgagcccggc gcttccttgc gatgatagtc
     1081 gacgatggcg cggcggaagg gttccgcaac cggcgtcttg acgtaggaaa ccttggccag
     1141 cagttccttg tcgcgcggct tgaaaacggt ctggttggtc gcttcaaagg tggtggcctg
     1201 catgcagccc gatagcatca ggccggcagc cagaattccc agttttacgc tcagcgacga
     1261 cattgtgcac ttccaatcga aattctcgcg ttccaggcac cagcatggag cccaaacgcc
     1321 acgattccgt taaggttatt atcgccaaaa ccggtcacaa ttccagagtt tagattctca
     1381 tcccgcgttg cggcacgcgg ttgtgtcttt tttgccgcaa gttccgcggt tttcggctga
     1441 tttttgggca ggtgcgcccg taccgccgga ccagcgccac ctagtcgcca ccgagctgcc
     1501 gcaaatgggg ctcgacagac accaggcccc gtcatgaagc gcttgccaga atcgagctaa
     1561 gtcctgttgg gtagttcgtg cgctcagttc cggagtcctt catgttatcg atgttcgtcc
     1621 cctccgaagc ctcgctgaag aaagtcgttt gcgccgatct cgccgcgctg ccggagagcg
     1681 cggtctggat cgatctggtg aaaccgaccc cggcggagga ccacgccgtg gagcggctcg
     1741 ccgggatcgc ggtaccgacc cgggaggaca tgcaggagat cgagatttcc agccggctct
     1801 atatcgagaa cggcgcccgc tacatgaccg ccacgctgat gtgcgcggcc gacacccaga
     1861 atccgcgcac caccgcggtg accttcatcc tcgcggccca tcgcctggtg acggtgcgct
     1921 atgacgagcc aaaaccgttc atgctggtgg agaacaagct ggcacgcgca tgcgcgcccg
     1981 gcgtcaccgg cgaaatggtg ctgatggaat tgctcgatgc ggtgatcgac cgcaacgccg
     2041 acatcctgga gcgcgccggc agcgacatgg acaacatcag ccacgatatt ttcgagcccg
     2101 agggcgccgc gcgcaccggc cacgccaagc gctattccaa tatcctgatc gcgatcggcc
     2161 gcaagggcga tctcacctcc aaggtgcgcg agagcctggt ttcgatcggg cgggtggtga
     2221 cgttcgttgc cgccggtacc gaaggggtca aatggtccaa ggatatgcgc gagcagctga
     2281 agaccatgca gcgcgacgtg acctcgctga ccgaccatgc ctcctatctc tccaacaaga
     2341 tcaccttcgt gctggatgcg atgctcggcg tcgtcaatct cgagcagaac aacatcatca
     2401 agctgttttc ggtgatggcg gtggtcctga tgccgccgac cttgatcgcc tcgatctacg
     2461 gcatgaattt caagtcgatg ccggagctgg aatggaccca cggctatccc tacgcgctgg
     2521 tggcgatgct gctcgccgcg gtcgggcctt acctgttttt cacatggaag aagtggttgt
     2581 aggcatgatc ccgaagagca ctccgtgggc tggaaccggt tttcggcaag gatcctagct
     2641 gaatggacga tctcgctctt cgcctgcgag gggacgcccg gacgcgtaag agcggcagcg
     2701 cggggcggta atgaaggtta aggatgctat tcggttgctt gaaaaggaag gctggtttct
     2761 ggttgcgaca cgcggcagtc accggcaata caagcatccg gttcggccgg gacgtgtgac
     2821 ggttgctggt aaaccctctg atgatcttgc gccgggcaca ttgaacagta tcttgaagca
     2881 gtccggcctg aaggagcaac attgatgcgt tacgcgatcg tcatcgaaaa agccgacgcc
     2941 aattattcgg cctatgttcc ggatctgccg ggctgcattg ctaccggcga cacggttgcg
     3001 gcggtcgaga gtgaaattcg cgatgcgatc cgcttccata tcgagggcct tcaggccgat
     3061 ggcatcgtgg tccccgagcc gaccagcatc gccgagtaca tcgagaccta ggcgcttact
     3121 tttgtttatt tgatcgacgt tcaatcaagt tcgccagaag acggtcatcc ctatggtgcc
     3181 aacaatctcg accagctcgc gcaaaggcgg agcagagtta agaaagtgcg tcctggattg
     3241 aatgcagtac atcgcgccgt ccacctcaat ttctcccgta aaatttgagc gttggactga
     3301 acgtttacgc gacggctttc tgcgataacg ttgtctcaag gcgcgaggac agcaatggtt
     3361 gagaaagtca taacttcatc ggagaacgtg gtcgatttcg cgcgctatca agtggatcgc
     3421 aatgccggca aggcgcgggc gatctcggcc cgggcctgcc ggcattgcgg cgcggcgctg
     3481 tcggagggcg agagcgaaga cgagtgctcc agcgccttca acatcgacaa tgcccgcctt
     3541 cgctggcagg ccgcgcaaat cctgcgcgga ttgatttgag cgggtcgcgg cacgaaagtt
     3601 ttttgcggag tgagttcact tccgtcattc cgggatgcgc cacttggcgc aggcccggaa
     3661 tccatactcc ccgtcgtggt tatggattcc gggctcgcgc ggagcctgtc atcgggcggc
     3721 gcagagcgcc gacccgttgg tgcgccccgg aatgacgaga agctaaacgt gctgcccgcc
     3781 gttgatgtgg atctcggcgc cgttcacgta ggagctggtc tccgtgcaca gcacatagat
     3841 gatcttcgcc acttcgtccg gcgtgcccag ccgatgcagc gggatctgct gctcgacgat
     3901 cctttcggtg cccggcgata atatcgaggt gtcgatttcg ccgggcgcga tcgagttgac
     3961 gcggacgccg atgcggccga aatccgatgc catctcgcgg gtcaatgacg ccagcgccgc
     4021 cttcgaggtc gcataggccg cgcccgcgaa cggatgcacc ctcgagcccg cgatcgaggt
     4081 gacgttgacc accgagccct tcgcggcctt caattcctcg atcagcccgc gcgccatcat
     4141 gatcggcgcg aagaagttga cccggaagac atggctccag gtctcgatgt cggtatcgat
     4201 cgagccgagc cgtccgccgc cttccgtctt cggtgaaatc gcggcgttgt tgaccagcgc
     4261 gtgcagatcg ccgccctcca gccgctcgcg gatttccgaa atcgcccgcg tggtgtcggc
     4321 gtggtcggca aggtcgacct ggatgtggtc ttcgggtccc gcgccccacg ggcactcttc
     4381 cgggaacggg tgtcgcgagc aggtgatgac gcgccagccg gcgctggaaa accggatcac
     4441 ggtggcgtgt ccgatgccgc ggctggctcc ggtcagaagc agcgtacgcc gcggcgcgtt
     4501 ggtggagttc ggcatggaag tccttcaggc tctgggcgtc agggataaag ccgcgtcttg
     4561 gaccatggct gatccggctt agcgcggcgg aattcgatgc ggtcgtgcag gcggaacggg
     4621 cggtcgtgcc agaattcgag ccgcagcggc gcgatgcgcc agccgctcca ccccggcggc
     4681 cgcggcacct cgccgaggat gtgccgggcg gcaatttttg caatggcctg ctcgaacgca
     4741 aagcggcttt ccagcggctg cgactgcttg ctggcccagg cgccgatctg ggcctgcttc
     4801 ggccgggtcg cgaaataggc gtcggcctcg gcgtcggtca ccggcgacac gttgccgcgg
     4861 atccgaatct gccggcgcag cgatttccag tgaaacagta aagccgcctt aggatttgtg
     4921 gcgagttcgc gtcccttctg gctggcgatg tggctgtaga acacgaaacc actggcatca
     4981 tagcctttca tcagcaccat tcgcacgtcc ggcagcccat cggcgtcgac ggtggccagc
     5041 gacatggcgt tgggatcgtt gggttcggcc ttcacagcct cggcgaacca ctcgccgaac
     5101 aaggcaaacg gttcctccgc cgcggtgaaa tcacctgatg ttaacggtgt ggggtgtttg
     5161 atggaggtcg tgtcggtcat gtcaggagtc cgagttgcgt tcgcccgcgg tccagagcgc
     5221 gttgcagcac ttatgtaggc tcgccctata taaggcatgg ggacgcattg gcctatcggc
     5281 gatcgccccg gcgggcaccg cgatgatact gattctaatc ggcgccggct cggggggctg
     5341 cagcctgtcc cgcaccgacg gcgccttcgc caggatggaa gaccatgggg tcaccggttc
     5401 ggtgggcctt gcgcaggcag gcggaccgat gccaaccgag agcgatctcg cccttgcccg
     5461 caatgccgcc tccgacgtgc tgaccaaggg gaccaaggat tccagccagc cctgggagaa
     5521 tccggagacc ggcgcgcggg gctcggtgac gccgctggcg gaggcctatg cctcggatgg
     5581 acgaacctgc cgggactttc tggcaagcta tgtcaacggc cggtcggaaa gctggctgca
     5641 gggcgccgcc tgccaatccg aacagggccg atgggaaatt catacgttga aaccgtggcg
     5701 gcggggttga acgatcaatg cccgttgcaa aaatgccacg aagaccccaa atgaaaattg
     5761 ggacgggccg atagcccgga gtctaagatc tgattcccta ataaggagac gtgacggatg
     5821 cgtgacccct atgaggtctt gggggtgccg cggggcgcca gcgctgcggc gatcaagagc
     5881 gcctatcgca agctcgccaa gaagcatcac cccgatgcca acaagaacga tccgaaatcc
     5941 gcggcgcgct tttccgagct gaattcggcc aacgagatca tcggcgacga ggaaaaacgc
     6001 aagcagttcg atcgcggcga gatcgatgcc gacggcaagc cgcgctttca gggctttccc
     6061 ggcggcgatc cgcgtggccg aggggcctcg cccggcggcg gcttcgaaac ccatacattc
     6121 cgcaccggcc aagggggttt tagcggcggt gccggtttcg aggacatcct caacagcatg
     6181 ttcggcggtg ctgcggcacg cggggcgcga cccggcgccg gtggcagctt cgagttcgac
     6241 cccggcggca tcgcggtcga tctcgatctc aacgtcgcca tgaccgtgtc actggaagaa
     6301 gcggtccacg gcgtgacgaa gcgcgtccgg ttgccgaccg gcaaggaact cgatgtcaag
     6361 attcctgctg gagtcgttgc cggccagcag atcaggctga aggggcaggg cgagaccgcg
     6421 cccggtcacc gccccggcga tctcctgatc acggtcggca ttgcgccgca tgcgatcttc
     6481 aaggtcgacg gcagcgacct gcgggtcgat ctgccgatca cgctgtatga agcggtgctc
     6541 ggcggcaaag ttcgcgtgcc tactttgggt agcgcggtcg aattgtcgat accgaagaac
     6601 acctcgagcg gccgcacctt tcgcctgaag ggcaaggggt tgccgaaagc gacgggaatg
     6661 acaggcgacc tgttcgtcac cactcgaatc atgctgccgg acggaaacga cgccgagctt
     6721 gaggcgttga tgcaaaagtg gcgcgacgga cacccttaca atccgcgcag cgacatgact
     6781 tgatcgcatt gcgccacccg aatggcgcca cccgaaatgg tgcaggggcg gatcaggcga
     6841 ccacaaatag aaaaagagtg cagcctcgac cgggggacca gcgcggcaaa ccccgatcga
     6901 ggccgcggcg tcgatcggcg gatcgaacgc gtctcaattt cgcgtgatgc ttggtgcgat
     6961 attgagacgt acgcatgttc caattcgaga ttttcaatgt cgaaattggg acataggccg
     7021 tgacatagtt cagccgcggg gcttttcggt ctggtcgtag accagccgcg cgacctgatt
     7081 cagccggtcc ttgtccaccg ggccggatag cacatagccg acgccgtctt ccgaccaata
     7141 catggcgccg ctgttctcgg cggcggtgta gcgcatctgc gccgtcccgg ttttcgcgcg
     7201 agaggtgtaa agcgtgaagc gttcgcccga tacgctctca tacatcaaga acgatgccgg
     7261 gccggtaggg ccgggcaaca gccgtccgcc aaccagcttc aaccctgtcg cgtccagctc
     7321 cggtgcatgg acggcccagc cgcagcgctt ggtcagccat tgctggagat gcgtgcgttc
     7381 gcttccgggc acctcgaccg gatggcgaac ctcgacgaca taaagccggt atgcgtccag
     7441 cgcatccacg gtcagtttct ggaacgccga cggcgaggcc gccgcgccgc gcgccagcca
     7501 gccgacaccg ccgccgccca cgaatgccag cagcgtcgcg gcaatcgcac cgtagagcca
     7561 tctgcgcggc tgccgctcca gccgctcgat ctcaagccgc ttcggcaccg cttcgtcggc
     7621 gacctgatcg taacgcgcat gcaacgcttc cgccatcgcg cgccatgatt gcacccgctc
     7681 ggcatcatcg ggatgtgctg agagccatgc ctcgacatcg ccgcgacgct ccgccggcag
     7741 ttcgttgtcg acgtaggcgt gcagctcgtc ttcggtgatg gagatctgtg ggtcggtcat
     7801 tgtcgtttgt cttctctgtc ttgtctgatc aacgtcgtat ctgaccaatg tccttgcggt
     7861 ttgcagtcat cgctgcgtaa tggctccgcc atcgccgctg gagcagcgaa acgcgcctcg
     7921 aagcacagcg cttactcctg atctctgtca tcttcgaggc tcaccgaagc ctgtggtgca
     7981 accgcaccgt cgcagttgcg gcgaaacgac gacatgatta cgcatgcata ccggttacgt
     8041 tgctgacgca tcacaactgc tcgcatcata gctgccatca tttcacccgc cgcagcgcga
     8101 cgcgctcgcc ttcgagcgag gctttcacat gggcgcgggc gcgcgcaagc cgggacatta
     8161 cagtgccgat cggcacgccc tgaatgtcgg cgacctcgcg gtagctcaat ccttccagca
     8221 tcaccaagag cagcaccgcg cgctgctctt cgaccagggt tgcgagcgcg cgggcaatgt
     8281 cgcggccttc cgcttcggtt ccgctggcgt cgggattgtt gtcgagcagc ggcatgaatt
     8341 gcggccgccg cgccagcgac ctccgcctgt tcttgttgag gttggtcagg atggtgtaga
     8401 gccagcttct gacgtcgccg ccgagaaaca gccgttccga acgcagcgcc cgcaccagcg
     8461 tgtcctgcac cagatcgtca gcgatatcgg catcgcgcgc cagcgcgcgt gcatagcggc
     8521 gcagcgccgg tatcatggct tcgatacttt gacgaaacgc ggtcatctca tctcgatctt
     8581 gggttgtccg ctcggcgtcc tgtaccggca acgcctgaca ccaagataac accgaaatga
     8641 cgccactatt ccggctgtta gggctgcgag accaacagcc ttgaacggag gcccggattt
     8701 gggctgtacc ggccggaagt gctggtgtac ctccagacct gatcaaggtc gattggaaac
     8761 cagatgtcgc aaagttcagg gctgatgcag ggcaagcgcg gagtgatact cggcgttgcc
     8821 aatagccgct cgattgcctg gggtatcgcc aaggcctgcc atgcggccgg tgcggaaatc
     8881 gccctcacct ggcagggcga cgcgctgaag aagcgggtcg agccgctggc gaaggaactc
     8941 ggcggcattc tgctcggcca ctgcgacgtc accgatcccg caaccatcga cgcggtgttc
     9001 gacgtgctca gggaaaaatg gggcaagatc gatttcgtgg tgcacgccat cgcgttctcc
     9061 gacaaggatc agctcgacgg ccgctatctc gaaaccaccg ccgacaattt ttcaaagacc
     9121 atgctgatca gctgctactc gctgaccgcg atcgcgcagc gcgccgaaaa gctcctgatc
     9181 gacggcggct cgatcctgac gctgacctat tacggcgccg agaaatggat gccgcattac
     9241 aacgtgatgg gcgttgccaa ggcggcgctg gaggcgagcg tgcgctatct cgccgccgat
     9301 ctcggcgaga agaacattcg cgtcaacgcg atttcggcgg ggccgatcaa gacgctggcg
     9361 gcatccggta tcggtgattt ccgctatatt ctgaagtgga acgaatacaa cgcgccgatg
     9421 cgccgcaccg tcacaacaga ggaggtcggc gacagcgcga tgtatctctt gtcggatctg
     9481 tcgcgcggcg tcaccggcga ggtgcatcac gtcgattccg gctaccacgt cgtcggcatg
     9541 aagcgcccgg acgcgccgga tatctcgctg agcaattcgt tgagcaagga atagtcaagc
     9601 ttacgatgcc ggcgccgacg atctactaca ttcgccatgg cgagactgaa tggaatgcgg
     9661 ccggac
//
LOCUS       DASWGM010000490         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_312944,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000490 DASWGM010000000
VERSION     DASWGM010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312944"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            813..>1702
                     /locus_tag="VGA15_14100"
     CDS             813..>1702
                     /locus_tag="VGA15_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588862.1"
                     /translation="MFARILSLLAALAAAILPAPAALAQISDDVVKIGVLTDMNGPAS
                     TPTGQGSVTAAQMAVDDFGGKVLGKPITVIVGDHQLKPDIGGGIARRWYDTEQVDLIV
                     DVPVSAVGLAVQNVANDKKRMFITHSTGAADFHGKFCSPYAIQWVFDTRALAVGTAQE
                     VVKRGGDSWFFITDDYAFGHSLERDASAVIIKNGGKVLGSVRPPFATPDLSSFILQAQ
                     ASKAKIIGIAGGPPNNVNEIKTAGEFGVLKGGQQMAGLLALITDIHALGLPAAQGLLL
                     TTSFYWDMDDKTREWSKRYFA"
BASE COUNT          329 a          503 c          552 g          318 t
ORIGIN      
        1 tggacgcggc agcgttgagc gtgcggcgtg atggcagggc gggttcttaa agagcctgtg
       61 agcgatcaca agcacgcgga cgagagatgt tgtcgcggac ggcaaaatcg tgtggtcctg
      121 acgccccgac gctggcgtca agtttcgcgg aggtttttat cggccctacc gggcagtgag
      181 aaaaccttaa tccgcgaggc gacggtggca aaagagcccg gtcaccgggg agagcacgac
      241 ataggccgtt aaaaccattg cgcagggaat gccgggtgtt ccggggtacc tgtggtgact
      301 accgtgtgct tactaccaat gcacacgggc tgcgggtgca gcgggcaccc ggcgttccct
      361 gcgccctctt cgggcgaaag aattcatgca cagctcgggc gcatcgcgtc gcgagcgcga
      421 agttgtttct gcgaatgacg gctgctgttt gaaaattgaa tccgaggcaa ttcgttgggc
      481 gcgaacgcgc cacactttca gccgtcgtcg cccgccaccg ggtcggcgcg gcgcgccgcc
      541 cgatgacagg ctccggccgg gacccataat cccaggcggg tgtggttgcg gaaggtgtcc
      601 gctttcgtgc ctcatcgaga cgccacgggt tatgggtccc ggccttcgcc gggacgacgt
      661 agtgaaagat ctctctttca atccgcagtg tcctgaatca gaaattcgct ttgacgcgga
      721 ctaaagaata gttgcaagaa tccgaaccgg ctgagctaga ttccggtcaa gcaacatggc
      781 cgatgcgccg agtggtaacg ggaggaaaca agatgttcgc cagaattcta tccctgcttg
      841 cggcgctggc cgccgcgata ttgcccgcgc cggcagcact tgcccagatc tcggacgacg
      901 tggtcaagat cggcgttctc accgatatga acggcccggc gtccacgccg accggccagg
      961 gctcggtcac ggccgcgcaa atggcggtcg acgatttcgg cggcaaggtg ttgggcaagc
     1021 cgattaccgt gatcgtcggc gatcatcagc tcaagccgga tatcggcggc ggcattgcgc
     1081 ggcgctggta tgacaccgaa caggtcgacc tgatcgtgga cgtgccggtg tcggcggtcg
     1141 ggctcgccgt acagaacgtc gccaacgata aaaagcggat gttcatcacg cattcgacgg
     1201 gggccgccga tttccacggc aaattctgtt cgccctatgc catccagtgg gtgttcgaca
     1261 cccgcgcgct cgcagtcggc accgcgcagg aagtggtgaa gcgcggcggc gacagctggt
     1321 tcttcatcac cgacgactat gcgttcggcc actcgctgga aagagacgct tccgccgtga
     1381 tcatcaagaa tggcggcaag gtgctcggct cggttcggcc gcctttcgcc acgcccgatc
     1441 tgtcgtcgtt cattctccag gcccaggcct cgaaagcaaa aatcatcggc atcgccggcg
     1501 ggccgcccaa caatgtcaac gagatcaaga ccgccggcga attcggcgtg ttgaagggcg
     1561 gccagcagat ggccggcctg ctggcgctga tcaccgacat tcatgcgctg ggattgccgg
     1621 ccgcgcaggg cctgctgctg acgacctcgt tctactggga tatggacgac aagacccgcg
     1681 aatggtcgaa gcgttatttt gc
//
LOCUS       DASWGM010000491         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1720314,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000491 DASWGM010000000
VERSION     DASWGM010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1720314"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1150)
                     /locus_tag="VGA15_14105"
     CDS             complement(47..1150)
                     /locus_tag="VGA15_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX9588863.1"
                     /translation="MSVQPLFTPVRVGDLDLPNRIVVAPLTRMRAGPIDHVPTALQAE
                     YYAQRASAGLIVAEATAISPDGFGYADTPGLWSAEQVRGWRLVTDAVHVGGGRIIAQL
                     WHTGAIAHPDVRGGSQPLSASDVDLLHESVTPSGRKPAVAPRPMTTAEIRQTVVDYAR
                     AARNAMEAGFDGLEILAAYLYLIPQFLNRTTNRRTDEYGGSIENRARILFETVEAVLE
                     EVDPSRVVGPMDDGGPMAANDETLPITEYAVRKLDGHGLSHLLLMGNTTDFTGTPLEP
                     LMGDGMFRYFRPIFRGKLIANVGMTAERGNRLIAEGLADLVAFGRPYIANPDMVERLA
                     TGAPLAEVDWTTVYASGPRGYSDYPAIRRAAAE"
     gene            1336..>1541
                     /locus_tag="VGA15_14110"
     CDS             1336..>1541
                     /locus_tag="VGA15_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588864.1"
                     /translation="MPDTAIDPIPKQGKFRRALAAVGAWLQALDYTGFDYTLDRTERL
                     ERELAQLKEELRQSREASSSDAHPG"
BASE COUNT          296 a          463 c          510 g          272 t
ORIGIN      
        1 cggacactat tgatcggacg ggtgcgcatt agccctctgg tcgagttcac tcggccgccg
       61 cccggcggat agccggatag tcggaatagc cgcgtggtcc cgacgcatag acggttgtcc
      121 agtcgacttc ggcaagcgga gcgccggtcg cgagccgctc gaccatgtcg ggattggcga
      181 tgtaggggcg tccgaacgcg accaggtccg cgagcccctc ggcgatcagg cgattgcctc
      241 gttcggcggt cataccgacg ttggcgatca gcttgccgcg aaagatgggg cggaaatagc
      301 ggaacatgcc gtcgcccatc agcggctcga gaggggtgcc ggtgaagtcg gtggtgttgc
      361 ccatcagcaa gagatgcgac agaccgtggc cgtcgagttt gcgcacggca tactccgtga
      421 tcggcaacgt ctcgtcattg gcggccatgg ggccgccgtc atccatcggg ccgacgactc
      481 gcgatggatc gacctcttcg agcaccgctt cgacggtttc gaacagaatg cgagcccggt
      541 tttcgatcga acccccatat tcatccgtgc gacgattggt ggtccggttc aagaattgag
      601 ggatcaagta gagataggcg gcgagtattt ccaatccgtc gaatccggcc tccatggcgt
      661 tgcgtgctgc gcgcgcataa tcgacaacgg tctggcggat ctcggcggtg gtcatcggtc
      721 ggggagcaac cgcgggcttt cggccagatg gggtcactga ctcgtggaga aggtcgacat
      781 cggatgccga caatggctgc gaaccgccgc gcacgtcggg atgggcaata gcgccggtgt
      841 gccaaagctg cgcgatgatg cggccgccgc cgacatgcac ggcgtctgtg accaggcgcc
      901 atccgcgcac ctgttccgcg ctccataatc cgggagtatc ggcatagcca aagccgtcag
      961 ggctgatcgc ggtcgcttcc gcgacgatca gtccggccga agcccgctgg gcatagtatt
     1021 cggcctgcag cgcggtcggc acgtgatcga tcggcccagc gcgcatgcgg gtgagcggtg
     1081 ccacgacgat gcgattgggc agatccagat cgcccacgcg gactggggta aagagaggct
     1141 ggacggacat gacggcgctc ctggacaagg attaagcggt gacgacgatt ttgccggttt
     1201 gctcgcccgc ctcgatgaag cgacgggcgt caacgatcta taagtcagtt gaccaactag
     1261 atgggatatc cgaacctgtc aagccttgcg ttttgcgtgc ggcgagcccg gcatcgcgca
     1321 acgaggagct ttccgatgcc cgacacagca atcgatccga tccccaagca gggaaaattt
     1381 cgccgcgcac tcgccgccgt cggcgcttgg ctccaagcgc tcgactacac aggcttcgac
     1441 tacactctcg accgcaccga acgcttggag cgggagctgg cacagctaaa agaagagttg
     1501 cgccaaagcc gggaggcgag ttccagcgat gctcaccccg g
//
LOCUS       DASWGM010000492         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_172378,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000492 DASWGM010000000
VERSION     DASWGM010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172378"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VGA15_14115"
     CDS             <1..231
                     /locus_tag="VGA15_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HEX9588865.1"
                     /translation="QLIYCRIGNPTPEFLYLVLQRDGKTMRLFPCGGKSGIHVPLAII
                     DDILPDSVIELVIGAPEGLTAKVMIDLGFVEI"
     gene            257..>1640
                     /locus_tag="VGA15_14120"
     CDS             257..>1640
                     /locus_tag="VGA15_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9588866.1"
                     /translation="MIIGNGMAGARTVEEILARGGPDLFNIAMFGDEPFGNYNRIMLS
                     AVLDGSREATEIVLNPLAWYERNAIRLHAGIRATRIFRFARRVLGADGSEEPYDNLII
                     ATGSRSFIPPLLGLTRSDGSMKPGIFGFRSLEDCHHMEDYARGKKRAAVIGGGLLGLE
                     CAHGLQRFGLEVHVFHRSPHLMNQQLDSAAGAILKSLMEEMGVRVHLGADTRAVLGDE
                     RVSGLEFKNGEPLECDLVVFATGIKPNSEIASQAGLTVERAIVVNNQMRTVDDPRIYA
                     VGECVQHRGQTYGLVAPIWEQVKVLADHIAGANPKAAYHGSKVATRLKVAGVELASMG
                     LTEPGEDRDEVVQFTEPRRGTYKKLIIRDGRLMGGILLGDSSKAASLLQVFESSAPLP
                     EERIRLLFDIGAQPKRITLERMALDAQVCSCHAVNKGAIVECVKSGRRTLEAVREATR
                     AGLACGSCQPM"
BASE COUNT          326 a          497 c          520 g          297 t
ORIGIN      
        1 caattgatct actgtcgtat cggcaaccca accccggaat tcctatacct tgtactccag
       61 cgcgacggca aaacaatgcg gctgttcccg tgcggtggca agagcgggat tcacgtgccg
      121 ctcgcgatca tcgacgatat cctgcccgac agcgtgatcg aactggtgat cggggcgccc
      181 gaagggctaa cggcaaaagt gatgatcgac ctgggctttg tggagattta agcccatgga
      241 cgcaaagcgt aatctggtga tcatcggcaa cggcatggcg ggcgcacgca ccgtggagga
      301 gattctcgcc cggggcggcc cggatctgtt caacatcgcg atgttcggcg acgagccgtt
      361 cggcaattac aaccgtatca tgctctcggc cgtgctcgac gggtctcggg aggcgaccga
      421 aatcgttctc aacccgcttg catggtatga gcgcaatgct atccgcctgc atgcgggtat
      481 ccgcgccacg cgcatcttcc gcttcgcccg ccgcgtgctc ggagccgacg gatccgaaga
      541 gccgtacgac aacctgatca tcgcaaccgg cagccgctcc ttcattccgc cgcttctcgg
      601 gctgacgcga agtgacggca gtatgaaacc tggcatcttc gggtttcgct cgctcgaaga
      661 ttgccaccac atggaggact acgcgagggg gaaaaagcgc gcggcggtga tcggcggcgg
      721 tctgctcggg cttgaatgcg cccacgggct gcaaagattc ggtctcgaag tccacgtctt
      781 tcatcgctcg ccccacctga tgaaccagca gcttgattcc gctgccggcg cgatcttgaa
      841 gtcgctgatg gaagagatgg gcgtgcgagt tcatctcggc gccgacaccc gggcagtcct
      901 cggcgacgag cgcgttagcg gactcgagtt caagaacggc gaacccctcg aatgcgactt
      961 ggtggtcttc gccaccggaa tcaagcccaa ttcggaaatc gccagccagg ccggccttac
     1021 ggtcgaacgc gcgatcgtgg tcaacaacca gatgcgcacg gtggacgatc ctcggatcta
     1081 cgccgttggc gaatgcgtgc agcatcgcgg gcagacctac ggcttggtgg cgccgatatg
     1141 ggaacaggtc aaggtgctcg cggatcatat agccggagcc aatcccaaag cagcgtacca
     1201 cggttcaaag gttgccacgc gcctcaaggt ggcgggcgtc gaactagcct cgatgggact
     1261 gacggagccc ggcgaggatc gcgacgaggt ggtgcagttt accgagccga ggcgcgggac
     1321 ttacaagaag ctgattatcc gcgacggccg gctgatgggc ggtatcttgc ttggtgacag
     1381 cagcaaggct gcctccttgc tgcaagtctt cgagagcagc gcgccgctgc ccgaagagcg
     1441 gatccgcttg ctgttcgata tcggcgcgca gcccaaacgg atcacgctcg agcggatggc
     1501 gcttgacgct caagtctgca gctgccacgc cgttaacaag ggggccatcg tcgaatgtgt
     1561 caagagcggc cgccgcactc tcgaagcggt gagagaggcg acacgcgccg gccttgcatg
     1621 cggctcctgc caaccgatgg
//
LOCUS       DASWGM010000493         7238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_729975,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000493 DASWGM010000000
VERSION     DASWGM010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729975"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..970)
                     /locus_tag="VGA15_14125"
     CDS             complement(14..970)
                     /locus_tag="VGA15_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9588867.1"
                     /translation="MQSLAHRRCVNYGLMKHNGENVQQMRSSVALAIIGFIIVACALS
                     TNTILSRANAGEVPSFSLAFFRWMIVALGLAPFSVREIRAKWPAISPRLGMVAAAGFC
                     GMFLCGGPVYVAGVTTTAINIGLIMAAAPVNVLLIAWIIGFENIKPVQVASIAVALPG
                     VALILARGSLETLTTVNFALGDILVFIAMLGWTGYNLLQVRTLPDVSSIARTCLFAFA
                     GAGFSLPFCLHEALEAPQAVFSARAFLTYLFAAIVPGIGAYAGLAYLAAKFGSVRASL
                     MTYVVPIASVLLSMLFLGEGPSAYHFIGGLLILAGIWLSVRR"
     gene            1146..1409
                     /locus_tag="VGA15_14130"
     CDS             1146..1409
                     /locus_tag="VGA15_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9588868.1"
                     /translation="MLFVNRTSLDAPAAADIIKKVFKLTPAELRVMLTVVEFGGVPDT
                     ARNLNIAESTVKTHLGRIFAKTDTKRQAELVKLAAAFSPQLRS"
     gene            complement(1474..1617)
                     /locus_tag="VGA15_14135"
     CDS             complement(1474..1617)
                     /locus_tag="VGA15_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588869.1"
                     /translation="MALPAVLLLLKISVPSLLMKADPAVALFWNVVMPPTNHGVSGIK
                     EFS"
     gene            2003..2284
                     /locus_tag="VGA15_14140"
     CDS             2003..2284
                     /locus_tag="VGA15_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX9588870.1"
                     /translation="MHGTNNNGARPHTPLGLAKRGFSGIIHHLAADDAGSALSATELE
                     SRLIELGFVEGAKVEIMHEGIVGRDPIAVRVENVTIAVRRREAMAIIVA"
     gene            2302..4176
                     /locus_tag="VGA15_14145"
     CDS             2302..4176
                     /locus_tag="VGA15_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX9588871.1"
                     /translation="MEPPLMHLALVGTPNSGKTSLFNALTGSRQKVANYPGVTVERKE
                     GSFVTPLGRQVSVVDLPGTYSLRGRSPDEEITRDIVLGRASGEALPDLVLCVADSTNL
                     RLTIRLALELKRTGRPLLLVLNMFDIATRRGVSVDVAQLSEVLDIPVVTSIAVRKGGT
                     ADLLRRTDEIAAQTASSPVQQNLWQPLTVAELRATQREADRIIAATISLPSRPDTWTA
                     RIDAVVLHPVAGLAILALILFVMFQAVFAWAQPLMELLSDSFTALGQLVHDNLPAGLL
                     QSFLQNGVISGVGSVIVFLPQIIIIFLFILMLEDFGYMARAAFLMDRIMGGAGLHGRA
                     FIPLLSSFACAIPGIMATRVIDNRRDRLTTILIAPLMTCSARIPVYTLIISAFIPDQQ
                     VWGWVNLRGLVMFGLYAAGIASALGVSFLIKFFMWRDYAPAPFMLELPDYKMPRARSI
                     AIGVYTRAKMFLQRAGTTIFSMMVLIWFLASFPLAPAGAQDPAINYSFAAMIGKALEP
                     LLAPLGFNWQIAVALIPGMAAREVAVAALGTVYAIEGGKEAAEQIGQMLATKWSLATA
                     LSLLAWYIFAPQCASTLAVIRRETGSWKWMAITFAYMFALAYAASLATYNIARALGAG
                     "
     gene            complement(4221..5174)
                     /locus_tag="VGA15_14150"
     CDS             complement(4221..5174)
                     /locus_tag="VGA15_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588872.1"
                     /translation="MHQALRSTRSSSQLFGHREQATQHDYPFFVRMRSRRDDCCRSWR
                     DASLFEISFEISRPAYVRAARRHIWLSLWFPGLRGALGTAGGGGTSEQVERCPTKGSP
                     NMRASMAYFAGAGTVIAAIAGGVGGGLLIADMVSPKSPKQGVEQTRLERRMSPEPIAA
                     ANAPAEPVQYLDASQLPPSGAAVQAVPAQAQTQADNSVSQPPQPVDTAAVQPAAPAAQ
                     PVAHEQTAAADDAVARTPDADSRRAVEKRRAERHQQWADRRRHQPRQERDLQAVEEKV
                     REETEPRQEFAPEPETVTRVIHLFGRNENTWLKYLAKILGY"
     gene            5594..6748
                     /locus_tag="VGA15_14155"
     CDS             5594..6748
                     /locus_tag="VGA15_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9588873.1"
                     /translation="MNDLPNPEDDVPAGSEDGVALSLSGGGYRAMVFHVGALYRLNEI
                     GLLGRLARISSVSGGSITAGLLGLLWSKLQFDNGKAANFGLFVDGIRTMADTTVDAGA
                     VIGGIFLPGTISERVAQAYDDVLFKGAKLSDLPDDTGKKAPHFVINATNIQSSVLWRF
                     SRPYMADYRVGMVRNPDVALCSAVAASSAFPPVLSPMTLPIRQKVEATKGADLNLAPY
                     TTQAVLSDGGVYDNLGLETTKRFKTLLVSDAGQKIAPEPDPHHDWVRHSLRILDTIDN
                     QVRSLRKRTLIDSYKRGDHTGTFWGIRTDFADYKLATDPLKCLTRKPAPLAKIPTRLE
                     KMPRDIQNRLMNWGYAICDAALRAHVDAPLQKKLGIQITDTDKFPFPGGY"
     gene            complement(6863..>7238)
                     /locus_tag="VGA15_14160"
     CDS             complement(6863..>7238)
                     /locus_tag="VGA15_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588874.1"
                     /translation="AAPASVPFASPKPKVVAKPAPQKTYALLSDEQIAGIKERLKLSS
                     SQDSYWPAVESALRAVARKIHATRQTAPNATGMPIDPDAEEVQQLKSAAMPLLFQLRE
                     DQKREVRALARLIGLEKVASMI"
BASE COUNT         1436 a         2255 c         2145 g         1402 t
ORIGIN      
        1 tctcggatga ccgctagcgt cgcacgctga gccatatacc ggcgaggatg agcagaccgc
       61 cgatgaagtg gtaagcggaa gggccctcac ctaagaacag catggaaagc agcacacttg
      121 cgatcggtac gacgtaggtc ataagcgaag cgcgcacgga gccgaatttc gcggcaaggt
      181 acgccaaacc ggcatacgca ccaattccag gtacaatcgc ggcaaacaaa taggtgagga
      241 atgcacgagc cgagaacacg gcctgtggcg cctccaatgc ctcgtgcagg caaaacggca
      301 ggctgaaacc cgcacccgcg aaggcaaaca ggcaagtacg cgcgatggag gacacgtcgg
      361 gcaaagttct cacttgcaac aggttgtagc ctgtccagcc gagcatggcg atgaagacga
      421 gaatatcgcc tagagcaaaa ttgacggtgg tcagtgtttc cagtgagccg cgagccaaga
      481 ttaaggcgac gcccggcaat gctacagcta tgctggccac ctgcacgggc ttgatattct
      541 cgaagccaat gatccatgcg atcaagagca cgttgacggg cgccgccgcc atgatcagcc
      601 cgatgtttat cgccgtcgtc gtgacaccgg ccacatagac gggtcctccg cagagaaaca
      661 tcccgcagaa gcccgcggcc gcgaccatcc ccagacgcgg tgaaatcgct ggccactttg
      721 cccttatctc gcgaaccgag aacggtgcga ggcccagcgc aacaatcatc cagcgaaaga
      781 atgcgagcga gaacgagggc acctctcccg catttgcgcg tgaaaggatt gtattcgtag
      841 aaagcgcaca ggcaacgatg atgaagccaa tgatcgcgag tgccaccgac gaccgcatct
      901 gctgcacgtt ctctccattg tgcttcatca aaccatagtt cacacaacga cgatgagcca
      961 atgattgcat tcgtagtctg acgccctagg tccgcttagg atcaaaagcc gaagggctcc
     1021 cggcgagcaa aatgttttcc gctttgcccg accacatgca gacagttgcc aactcgtcat
     1081 tcgggtcgac aagctacgat gatgggcaat gagtcacaat ggctcgattc acgagaccaa
     1141 ccgcggtgct gttcgtcaac aggacctcgc tcgacgcccc ggcggcggcc gacatcatca
     1201 agaaggtctt caagctgacg cccgccgaac tgcgcgtgat gctcacggtc gtcgagttcg
     1261 gcggggtacc cgacaccgcc cgcaacctca atatcgcgga atcgacggtg aagacgcatc
     1321 tgggacggat cttcgccaag accgacacca agcgccaggc cgagctcgtc aagctggccg
     1381 cagccttctc tccgcagctc cggagctgaa tgcatcagct cccgcgcttc aaggcttgcg
     1441 ctcgcgcccg cctcgatgtg catccgcgcg ccgtcacgaa aattccttga tgcctgaaac
     1501 gccgtggttt gtcgggggca taacaacatt ccagaacagc gccaccgccg gatcggcttt
     1561 cattaacaac gatgggacgc tgatctttaa cagcaacagc actgccggca acgccacgat
     1621 cgagtccgat ggcgcaatcg ctttccagca gcgagcgagc ggcggcagcg cccggattgc
     1681 gatgacgggc gcctcatatt tacccccgcc cacataccac ccctagccgc gtgatgtgag
     1741 gttgccaatc atgttgtccg cggttcgcag cgccgacagg gtgagcgtcg gactggcggt
     1801 gctaccggtt gggaaacgtg cgcttttcat agtctcttaa aagtgactgt agtctcctaa
     1861 aagtaactat gccgccgggt ctgccgcatg tgcgacgaca aactggaggt gcccgcaacg
     1921 gctgctttac aatacttcta aggtgcgacc tctttcctct cgccggactt gacctagctg
     1981 cttgtcattg gaaagaaaga tcatgcacgg cacaaacaac aacggcgcac gcccgcacac
     2041 gccgcttggc ctggccaagc gcggctttag cggcatcatc caccatctcg cggccgacga
     2101 tgcgggctcg gcactatcgg caaccgagtt ggagagccgg ctgatcgagc tcggtttcgt
     2161 tgaaggcgcg aaagtcgaga tcatgcatga gggcatcgtc gggcgagacc cgatcgccgt
     2221 ccgggtcgag aacgtcacga tcgcggtgcg ccggcgcgag gcgatggcca tcattgtcgc
     2281 ctgacgagac ggtacagcgc catggaaccc ccgttgatgc atctcgcttt ggtgggcacg
     2341 ccaaacagcg gtaaaacctc gctgttcaat gcgctgaccg gcagccgcca aaaggtcgcg
     2401 aactatcccg gcgtcacggt ggagcgcaag gaaggctctt tcgtcacgcc actcgggcgt
     2461 caggtgtcgg tggtcgatct gcccggcacc tattcgctgc gcggccgcag ccccgacgag
     2521 gaaatcaccc gcgatatcgt gctcgggcgc gccagcggcg aagccctgcc cgacctcgtt
     2581 ctatgcgtgg ccgactccac caatctgcgg ttgacgattc gactggcgct cgaactcaag
     2641 cgtaccggcc gaccgctgct gctggtgctc aacatgttcg atatcgccac acggcgcggc
     2701 gtcagcgtcg atgtggcgca actgtccgag gtattggaca tacccgtggt gacatcgatc
     2761 gcggtgcgca agggtggcac cgccgatctg ttgcggcgga ccgacgagat cgcagcgcaa
     2821 acagcatcaa gtcccgtgca gcaaaatctc tggcagccgc ttacggtcgc cgaattgcgc
     2881 gccacccagc gcgaggccga ccgcatcatc gccgccacca tcagcctgcc gtcgcggccc
     2941 gatacctgga cggcgcggat cgatgccgtg gtgctgcacc cggtggccgg gctagccatc
     3001 ctggcgctga tcctgtttgt gatgttccag gcggtgttcg catgggcgca gccgctgatg
     3061 gagctgttgt cggactcctt caccgcgctg gggcaactgg tgcacgacaa cttgccggcg
     3121 ggcctgctgc aaagttttct gcaaaacggc gtgatttccg gcgtcggcag cgtcatcgtg
     3181 ttcctgccgc agatcatcat catcttcctg ttcattctga tgctggaaga tttcggctac
     3241 atggcgcgcg ccgcgttcct gatggaccgc atcatgggcg gcgccggcct gcacggccgt
     3301 gccttcattc ccctgctctc gagtttcgcc tgcgccatcc ccggcatcat ggcgacgcgg
     3361 gtgatcgaca accgccgcga ccggctgacc acgatcctga tcgcgccgtt gatgacctgt
     3421 tcggcgcgga tcccggtcta caccttgatc atttcggctt tcattcccga ccagcaggtc
     3481 tggggatggg tcaatctgcg gggcctcgtg atgttcggcc tctacgccgc cggcatcgcc
     3541 agcgcgttgg gcgtgtcatt cctgatcaag ttcttcatgt ggcgggacta tgcgccggcg
     3601 ccgttcatgc tggaattgcc cgactacaag atgccgcggg cgcgcagtat cgcgatcggt
     3661 gtctatacgc gggccaagat gttcctgcag cgcgccggca ccacgatctt ctcgatgatg
     3721 gtgctgatct ggtttctggc ctcgtttccg ttggccccgg ccggcgcaca ggaccccgcc
     3781 atcaactaca gtttcgccgc catgatcggc aaggcgctgg agccgttgct ggccccgctc
     3841 ggcttcaact ggcagattgc ggtggcgctg attccgggca tggccgcgcg cgaagtcgcg
     3901 gtcgcggcgc tcggcaccgt ctatgccatc gagggcggca aggaagccgc cgagcagatc
     3961 gggcagatgc tggcgacgaa atggagcctg gccaccgcgt tgtcgctgct ggcctggtat
     4021 atcttcgcgc cgcaatgcgc ctcgaccctg gccgtcatcc gccgcgagac gggaagctgg
     4081 aagtggatgg cgatcacctt cgcctacatg ttcgcgctcg cctatgccgc aagtcttgcg
     4141 acctacaaca tcgcgcgcgc cctgggcgct ggctgatcct gcgagcgcct cacctattca
     4201 gccgcgcgcc ccacgccaga tcaatagcca agtattttag ccaagtattt tagccaagta
     4261 ttttcattcc ggccgaacag gtgaataact cttgtaactg tttccggctc cggcgcgaac
     4321 tcctgcctcg gctcggtctc ttccctgact ttttcctcga ccgcctgcaa gtcccgctct
     4381 tgtcttggtt gatgacggcg cctgtcggcc cattgctggt gacgctcggc cctgcgcttc
     4441 tccaccgccc gcctcgaatc ggcgtccggc gtcctggcga cggcgtcgtc tgcagcggca
     4501 gtttgctcgt gcgccacggg ctgcgctgcg ggagcagcgg gctgcacagc tgcggtgtcg
     4561 accggctgcg gcggttgcga aacggaattg tcagcttgcg tctgcgcctg cgcaggaacc
     4621 gcctggacgg ctgcgcctga tggaggcagc tgcgacgcat ccagatattg caccggttcc
     4681 gctggcgcgt tcgccgcagc gatcggctcc ggcgacatgc gccgctccaa ccgggtctgt
     4741 tcaacgccct gcttggggga tttgggacta accatgtccg cgatcagaag accaccgccg
     4801 acaccgccgg cgatcgcggc gataacggtt ccggctcctg caaaataggc cattgaagca
     4861 cgcatgttgg ggctcccttt ggtcgggcaa cgctcaacct gttctgatgt tccgccgccg
     4921 ccggcagtgc cgagcgcccc ccgtaaaccc gggaaccaaa gcgacagcca gatgtggcga
     4981 cgcgctgcac gaacgtatgc cggtcgggaa atttcaaagg aaatttcaaa gagagaagcg
     5041 tcgcgccatg accggcagca gtcatcgcgg cgcgaccgca ttcgaacaaa aaaaggataa
     5101 tcatgctgag tggcctgctc gcgatgtccg aatagctgcg aggacgagcg ggtagagcgc
     5161 agcgcctggt gcatgcgcaa gaaccagccg ccggccgctt atttaagttg aacatcgcca
     5221 tatagtcgcg ttatgattta caccttagat actcacgtta tgattttgca gctctcggcg
     5281 ggacatcgcc tggcggtcaa aacgcttcgc aacaattgcg cgaagtttta tgatgcgtcc
     5341 ttggccgcca ccttgcgcgc cgagatttat tgaacggctt acgtttcagc gaagatggac
     5401 cgcacagcat cgcgaccggt gcgaaaattt ttcgactgga ctgagagcag tgccagcgcg
     5461 ttacgcactg gttgatcgtc gaatctattg ttcagcagat gccgggtaaa ggcggcaatt
     5521 tgagcaggaa ttattggacg gcctctgcga cggtcgaagc aggccaacgg agtatttcga
     5581 ggagtatttc gatatgaacg atttgccgaa cccggaagat gacgtccccg cgggatccga
     5641 ggacggtgta gcgctgtcgc tgtcgggcgg gggctaccgc gcaatggtat ttcacgtcgg
     5701 agcgctgtat cggctcaacg agattggact gctgggcaga ttggcgcgaa tctcaagcgt
     5761 gtcgggtgga tcgataaccg ccggcttgtt gggcctctta tggagcaagt tgcaattcga
     5821 taacggcaag gccgccaatt tcggtctgtt cgtcgacggc attcggacca tggctgacac
     5881 cacggtcgac gccggcgcgg tgatcggcgg cattttcctg ccgggcacaa tcagtgagag
     5941 ggtggcgcaa gcctacgacg acgttttgtt caagggcgcc aaattgagcg accttccgga
     6001 cgacaccggg aagaaagctc cgcatttcgt tatcaatgcc acgaacattc aatcctcggt
     6061 gctctggcgg ttttcccggc cgtatatggc tgactaccgc gtcggcatgg tccgcaatcc
     6121 cgatgttgca ctttgctcgg cggttgccgc ttcctcggcc tttcccccgg ttttgtcgcc
     6181 aatgacgttg ccgatccgcc agaaagtcga agcaacaaag ggcgccgatc tcaatctggc
     6241 gccctacacg acgcaagccg tgctgagcga cggcggcgtc tacgacaatc tcggactgga
     6301 gacgacgaaa cggttcaaga ccctgctggt cagcgacgcc ggccagaaga tcgcgcccga
     6361 gccggatccg caccacgact gggtacgtca ttcgcttcgg atcctcgata cgatcgataa
     6421 tcaggtccgg agccttcgca aacgaaccct cattgattca tacaagcgag gcgaccacac
     6481 cggcacattc tggggtatcc gcacggattt cgccgactac aagctcgcga ccgatcccct
     6541 gaagtgcctg acccgaaagc ccgcacctct ggcgaaaatt ccaactcgtc tggaaaagat
     6601 gccgcgggat atccagaacc gtctgatgaa ctggggatac gcgatctgcg atgcggcgct
     6661 tcgcgcacat gtcgacgcac cgctgcagaa gaaacttgga attcaaatca ccgataccga
     6721 caaatttccg tttcccggcg gctactgatc ccgaccggca cgcttttcat cgactatgaa
     6781 tttttggcat tcatttcgct cgaacaagcg atcgcacgct atccgcttcg acgccgcaat
     6841 gaatagggtg aacgcgcgcc gctcaaatca tcgacgcgac tttttccagt ccgatcaggc
     6901 gggccagcgc gcgcacttcg cgcttctggt cttcgcgcag ctggaacaac agcggcatcg
     6961 cggccgattt cagctgctga acttcttcgg cgtcgggatc aatcggcatg ccggtcgcgt
     7021 tgggggcggt ctgccgcgtc gcatgaatct tgcgggccac cgcacgcagc gcgctttcca
     7081 ccgccggcca ataggaatcc tgcgatgagg aaagtttcag gcgctccttg atgccggcga
     7141 tctgctcgtc actgagcagc gcataggttt tctgcggcgc cggcttggcg acaaccttcg
     7201 gcttcggcga cgcaaacggc accgacgccg gcgcggca
//
LOCUS       DASWGM010000494         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1281731,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000494 DASWGM010000000
VERSION     DASWGM010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281731"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VGA15_14165"
     CDS             <1..1174
                     /locus_tag="VGA15_14165"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9588875.1"
                     /translation="VGEFSAHKIGEEELLAVERNACPGAGSCGGMYTANTMSSAIEAM
                     GMSLPSSSTMAAVESEKADSAAKSGEVLVEAIRRHLTPRQIMTRKAFENAIAVVMAIG
                     GSTNAVLHLLAIAHAADVPLTIDDFETIRQRVPVLCDLKPSGRYVTTEFHAAGGVPQV
                     MKMLLNHGLLHGGEMTISGKTIAETLADVSDAPPKSQAVIRQWEDPMYPQGHLAILKG
                     NLATEGCVAKITGIKHTEITGPARVFDREEDALDAILAGKIKKGDVLVIRYEGPKGGP
                     GMREMLSPTSAIIGAGLGDAVGLITDGRFSGGTYGLVCGHVAPEAAVGGLIALVHEGD
                     SITIDAQKRLLQLNVPENEIARRRAAWTPPKPRYTRGVLAKYAAQVSTSSLGAVTD"
     gene            complement(1521..1880)
                     /locus_tag="VGA15_14170"
     CDS             complement(1521..1880)
                     /locus_tag="VGA15_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588876.1"
                     /translation="MSGVWEGTGLTDCTGFQAEPHRCNAVVNVTLTMLQEQERVTGFY
                     RCATGTVMCRQFLETGVIKAGSMTGRLLRFRVMLEDGESCIFQATPQSDRITGAYLCL
                     NGAAIMEQGRWDLERAY"
BASE COUNT          489 a          736 c          823 g          436 t
ORIGIN      
        1 ggtgggcgaa ttcagcgcgc ataagatcgg cgaagaggaa ttgctggcag tcgagcgcaa
       61 cgcatgcccg ggcgcgggct cgtgcggcgg gatgtacacg gccaacacga tgtcgtcggc
      121 gatcgaggcg atgggtatga gcctgccgtc ctcgtcgacg atggcggccg tggagagcga
      181 gaaagccgat agcgcggcca agtcaggcga agtgctggtc gaggcgattc gcaggcatct
      241 gacgccgcgc caaatcatga cgcgtaaggc gttcgagaat gcgattgcgg tcgtgatggc
      301 gatcggcggc tcgaccaacg cagttctaca tctgttggcg attgcccatg ccgccgatgt
      361 cccgctcaca atagatgact tcgaaacgat tcgacagcgc gtgccggtgc tgtgcgattt
      421 gaagccttcg ggccgttacg tcacgacgga attccatgcc gccggcggtg ttccgcaagt
      481 gatgaagatg ctgctgaacc atgggctcct gcatggcggg gagatgacga tcagcggcaa
      541 gactatcgcg gagacgctgg ccgacgtttc cgacgcgccg ccgaagagcc aagcggttat
      601 ccgtcagtgg gaagacccca tgtatccgca agggcacctg gcgattctca agggcaatct
      661 cgcgaccgag ggatgcgttg ccaagattac cgggatcaag cacacggaga tcacgggccc
      721 ggcgcgcgtc ttcgatcgtg aggaagacgc tctcgatgct atcctcgccg gtaagataaa
      781 aaagggcgat gtgctggtga ttcgatacga ggggccaaag ggcgggcccg gaatgcgcga
      841 gatgctctcg ccgacctcgg cgattatagg agcgggttta ggtgatgcgg ttggacttat
      901 caccgacggc cgcttctccg gcgggacgta tggattggtg tgcgggcacg tcgcaccgga
      961 agcagcagtc ggcggactta tcgcgctcgt ccatgagggc gactcgatca caatcgatgc
     1021 gcaaaagcgc ctgttgcagc taaacgtccc ggagaatgag attgcgcgcc gacgcgccgc
     1081 ctggacgccg cccaagccgc gttatacgcg gggcgtgcta gccaagtacg ctgcacaggt
     1141 atccacgagc agtctcggtg cggtcaccga ttagatcgcg cgaacccgag aatttgcgaa
     1201 gcagcggcgc cctttgggcc gccgctgctt cgcgttttga gagcagggga accgaggccg
     1261 cctcacggcg gcctcggttc ccctgccacg tctcggcagc atggccgggc gccgccgtca
     1321 ggcggtgccc ggccatgctg cccttgcctt ggcgtgcgcg cacgccaagg ccctggttgc
     1381 ctctaggcgc gccgccgccc cggcggcgtc gcgcctagag gcatgggaga agcgcatgca
     1441 gggtgtggcc tgtcagggcg acaccctgcc tcgcgcgagg gcggcgcggg cattcgctgg
     1501 agaaccacgt cgagtcgaat tcaatatgcg cgctccagat cccagcgccc ttgttccatg
     1561 atcgccgcgc cgttgaggca gaggtatgct cccgtgatgc ggtcggactg tggcgtggct
     1621 tggaaaatac agctctcgcc gtcttccaac atgacgcgga accgcaagag tcgtcccgtc
     1681 atcgacccgg ccttgatgac accggtttca aggaactgac ggcacatcac cgtgcccgtc
     1741 gcgcatctgt aaaagccggt cacgcgttct tgctcctgga gcatcgtgag cgtgacattg
     1801 acgaccgcgt tgcaacgatg aggctcggcc tggaatccag tgcaatccgt aagccccgtg
     1861 ccttcccata cgccactcac gtttgtgttt cgaggggtcg ccgagtcgga ttgacccggc
     1921 gaggaggcgg aatagcctcc cggcgccgcg gcaacccgaa ctgtggggtc atcagcggtc
     1981 gcgcacccac tggcaagcgc aagaatggcc acaagcccaa catacgagac aacgcggcca
     2041 ggaaacatgg atggcccccc agaatgaacg tgcctttcgg acactaggta ggtggcgaag
     2101 cgccagtcaa ggcagagaag cgagcaaggc gccgatgttt gttcaaaacg cgtttgttcg
     2161 ccaaactgct gttgttcgct aaggctgtgg cccgttctgg ccgggcttct cgagcgtcga
     2221 ttccaccttc atcgtacgcg gcggtccgga ggggcggcga tcacgttgcg gaatgcctcg
     2281 gcaaagactg atatacaggt agccaggaat gacagtcaga gcgcgcgcaa ggcaatggtg
     2341 cgacggaacc gagcggagaa aaggcagcga ccgtgaaagc cggcggtaac ttcttcaatc
     2401 tttacaatca tgatttcgtc cgcgcggccg tagggattcc cgcggtgcgc gtggccgacc
     2461 ccgcattcaa cggcgaacag atgc
//
LOCUS       DASWGM010000495         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1848035,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000495 DASWGM010000000
VERSION     DASWGM010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848035"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VGA15_14175"
     CDS             complement(<1..827)
                     /locus_tag="VGA15_14175"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX9588877.1"
                     /translation="MTDVLISAGELANLVSKEPCVIVDTRNPDAYGAGHIPNAVNVHE
                     IFTYLATSTPEGIHELKTKFAEAFGKAGLSGNETAVIYEQSMNSGFGQSCRGYYLLTM
                     LGYPKIKVLHGGFDAWAASGSPVTTEVPKPAPASFSIVPDASDILIDAKTMLAAVGKS
                     GIALLDVRDVDEWIGESSSPYGKDFCPRKGRIPGAVWLEWYRMMKPTAEGPRFKSKDE
                     ILADCATVGISENTPVYLYCFKGARASNTFLALKNAGVKDVRMYFGSWNEWSRDPSLP
                     "
     gene            complement(889..2010)
                     /locus_tag="VGA15_14180"
     CDS             complement(889..2010)
                     /locus_tag="VGA15_14180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9588878.1"
                     /translation="MSSVAVSRLAVDDEPDLRRDSIVDQVASIARRELAPIAADIDDG
                     AVYPADLLRRLGDAGAWGSHRPNDGAADLRCAIQSIAALGEVCGATAFMAWCQNTLVW
                     YAANSGNPALAGFTNDVATGKILGGTGLSNPMKSFFGIERLKLKGRKVDGGYVVRGAL
                     PWVSNLGADHFFGTIFEIEDRPGEIVMFLADCSDPAITLQPCKPFLAMDGTGTYSVQF
                     RDAFISSDHILAEPAGPFVKTIRSGFILLQAGMGLGLIKDCVAIMNEVAQPLGHVNRY
                     LPQQPLDFGEMLSDLESETMELAADPYNADDSYWRRVLALRLNLGEASVAAAHTAMLH
                     CGARGYLKSHRVQRRLREAYFVAIVTPATKQLRKMLAEV"
     gene            complement(2087..2629)
                     /locus_tag="VGA15_14185"
     CDS             complement(2087..2629)
                     /locus_tag="VGA15_14185"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX9588879.1"
                     /translation="MTAVAQKTVLTGCLAPIDKQGLEQLIANGKANPQVIKTLKCRTV
                     AEGKFRHANYIRNLPAYIVDEPPGLLGDDTAPNPSEASLAALGSCLAVGLHANAVHRG
                     WIVNKLELQLEGDLNITAVWGTGDVSEKPVGFTDVRVKVDMECEGVPQSEIDALVAHV
                     KKWSPVANTFTRPVNLEVSA"
     gene            2858..3523
                     /locus_tag="VGA15_14190"
     CDS             2858..3523
                     /locus_tag="VGA15_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9588880.1"
                     /translation="MGKSSSKKKSAAAPLAAGDDESARGRLLGAATHLFCKNGITATG
                     IDAIIHEAGTAKTTLYKLFGSKTNLVHAVLESEGRQWREWFIGAMESGGGDAQAKLTR
                     IFPALKSWFGEERFYGCPFINAVAEHDKDQKQFRAIAMRHKKVVLAHIEKLAAEMGAA
                     EPELLAHQLALLIDGAIVAAMVSRNPGVADTAGLAAGSLFAPSKLKKPKRAGNAAAQL
                     MAV"
     gene            complement(3776..>4042)
                     /locus_tag="VGA15_14195"
     CDS             complement(3776..>4042)
                     /locus_tag="VGA15_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX9588881.1"
                     /translation="TTDRRFAGQFKRQTPVGRHIPDFVSFVHRIAIELINADETETIK
                     KDRSLRQAWLEARGYRVITMHVAAVEGDVAAELDRLESSLLKCP"
BASE COUNT          802 a         1237 c         1233 g          770 t
ORIGIN      
        1 ggcagcgacg gatcccggga ccattcgttc cacgagccga aatacatccg gacatccttc
       61 acgccggcgt ttttgagcgc gaggaaggtg ttcgatgccc gcgctccctt gaagcagtaa
      121 agatagaccg gggtgttctc cgagatgccg acggtcgcgc aatcggccaa aatctcgtct
      181 ttcgacttga agcgcggccc ctccgccgtc ggcttcatca tgcggtacca ttcgagccag
      241 accgcacccg ggatacgccc cttgcgcgga cagaaatctt ttccataggg cgaagagctt
      301 tcgccgatcc attcatcaac gtcgcgcacg tcgagcaaag cgatgccgga cttgccgacc
      361 gccgccagca tggttttggc gtcgatcaga atgtcgctgg cgtccggcac gatcgagaac
      421 gaggctggcg ccggcttcgg cacctccgtg gtgaccggcg agccgctggc agcccaggcg
      481 tcgaacccgc cgtgcagcac cttgatcttg ggatagccga gcatggtcag caggtaatag
      541 ccgcggcagg actggccgaa cccggaattc atcgactgct cgtagataac ggcggtttcg
      601 ttgcccgaca gcccggcctt gccgaacgct tcggcgaatt tcgtctttaa ctcgtgaata
      661 ccttcgggcg tggatgtggc aaggtaggtg aagatttcat gcacgttcac ggcgttcggg
      721 atgtgtcccg cgccatacgc atccggattt cgcgtgtcga cgatcacaca cggctccttg
      781 gatacgagat tggcaagttc gccggcagag atcagaacgt ccgtcatggc aggcctctcc
      841 ttttctgtgg ttgcaagttg gggtcgatcg gaaatttttc tttaaagctc acacctccgc
      901 cagcatcttg cgaagctgct tggttgccgg agtgacgatg gcgacgaagt acgcctcgcg
      961 aagcctgcgc tgcacgcgat ggctcttcag gtagccgcgc gcgccgcaat gaagcatcgc
     1021 cgtatgcgcg gcggcgacgc tcgcttcgcc aaggttcagc cgaagcgcca ggacgcggcg
     1081 ccaatagctg tcgtccgcgt tgtacgggtc ggctgcaagt tccatggttt ccgattcaag
     1141 atcggaaagc atctccccga aatcgagcgg ctgttgcggc aggtagcgat tgacgtggcc
     1201 cagcggttga gccacttcgt tcatgatcgc gacgcaatcc ttgatcagac caagacccat
     1261 gccggcctgc agcaggatga aaccgctgcg aatcgtcttg acgaacggcc ccgccggttc
     1321 agccaggatg tgatcgctgg atatgaaagc gtcgcgaaac tgcacgctgt aggtaccggt
     1381 gccatccatc gcgagaaacg gcttgcatgg ctgcagcgta atggccggat cggaacaatc
     1441 ggcgagaaac atcacgatct cgccgggccg gtcttcgatt tcgaaaatcg tgccgaagaa
     1501 atgatcggcg ccgagattcg atacccatgg cagcgcaccg cgcacgacgt agccgccgtc
     1561 gaccttgcgg cccttcagtt tcagcctctc gattccgaaa aaactcttca tcggattgga
     1621 aagaccggtg ccgccgagaa tctttccggt cgcgacgtcg ttcgtgaatc cggcaagcgc
     1681 cggattgccg gaattcgccg cgtaccagac cagcgtgttc tggcaccagg ccatgaaggc
     1741 ggtcgcgccg cagacttcgc caagtgccgc aatcgattgg atggcgcaac gcagatcggc
     1801 cgcgccatcg ttcggccgat ggcttcccca cgccccggca tcgccgaggc gccgcagcag
     1861 atcggccgga tagacggcac cgtcatcgat gtcggcggcg attggcgcga gctcccttcg
     1921 cgcaatcgac gcaacctgat cgacgattga atcccgccga agatcgggct cgtcgtcgac
     1981 cgctaggcgc gatacagcta ctgaactcat cggactctcc gcaccggcgg tttttcagga
     2041 agcgacgcaa aggactgcac ggcaaatgac agcaagcttg cgacctctag gcgctgacct
     2101 cgagattgac cggtcgcgta aacgtgttgg ccaccggcga ccacttcttg acgtgcgcca
     2161 ccagcgcgtc gatctcgctt tgggggacgc cctcgcattc catatcgacc ttgacccgca
     2221 catcggtgaa gccgaccggc ttctcgctga cgtcgccggt gccccacacc gccgtgatgt
     2281 tgaggtcgcc ctcgagctga agctcgagct tgttgacgat ccatccgcga tgcaccgcgt
     2341 tggcatgcag cccgaccgca aggcatgagc ccaaggcagc caacgaggct tccgacggat
     2401 tgggcgcggt atcgtcaccc aacaggcccg gcggctcgtc gacgatataa gccggaagat
     2461 tgcggatgta gttggcatgg cggaacttgc cctcggccac cgtcctgcat ttcagcgtct
     2521 tgatgacctg ggggttggcc ttgccgttcg cgatgagctg ctccagccct tgtttgtcga
     2581 tcggggctag gcatcccgta agcaccgttt tttgagcgac cgccgtcatt ttttctctcc
     2641 ctaggggtgg ataccgaacg gtctgtatcc tgcacgaacc atgccagaca cggcggaaac
     2701 gaaaacgcga agttttgaaa tcgcttagcc cgcgctgcct tcggattgat caggggccgg
     2761 ctgctcaaac gccatgcaat tgccgcctcc gcgcgattat tttgagcgca tgaagatgcg
     2821 acttgatgaa aacgccgcgg ttcgatttac tgcgcggatg ggcaaatcat cttcgaagaa
     2881 gaaatcggcc gcggcacccc tcgccgcagg cgatgacgaa tcggcgcgcg gacgcctgct
     2941 cggcgcggcg acgcatctgt tctgcaagaa tggaatcacc gcaaccggca tcgacgccat
     3001 catccatgaa gccggcaccg cgaagaccac gctctacaaa ctgttcggtt cgaagaccaa
     3061 cctcgtgcac gcggtcctgg agagcgaagg caggcagtgg cgcgaatggt tcatcggggc
     3121 catggaatcc ggcggcggtg acgcgcaggc gaagctgacg cgaatcttcc cggcgctaaa
     3181 gagctggttc ggcgaagaac gtttctacgg ctgcccgttc atcaacgcag tcgcggagca
     3241 cgacaaggat caaaaacagt tccgcgccat cgcgatgcga cacaagaaag tggttctggc
     3301 gcacatcgag aaactcgccg ctgagatggg cgcggcggag ccggaactgc tggcgcatca
     3361 gctcgcattg ttgatcgacg gagcgatcgt tgcagcgatg gtgtcccgca atcccggggt
     3421 cgcagacacg gccgggcttg ccgcagggtc cttgttcgcg ccttcaaagc tgaagaaacc
     3481 gaagcgagcg ggcaacgcgg cggcgcagct gatggccgtg tgagcggcgg tttaaccgtc
     3541 cctgcagccg ttcaaaccag cgaccgcgcg acatgcgccg cgcgagttca gcgccacctc
     3601 tcccatgggg agaggtcgga tcggatcggc gacgcgtagc gtcttccggt gcgatccggg
     3661 tgaggggtta cagcctaacg agagaccgta ccccgaccct ctcctaacgg gagagggagc
     3721 gcacttccgt tgcttcaacg tcgtcacaaa catttcaatc taagtggatc agactctaag
     3781 ggcacttcag gaggctggat tcaagccggt cgagttcagc cgccacatcg ccctcgacgg
     3841 cggcgacatg catggtgatg acacggtagc cccgcgcctc cagccatgcc tgccggaggc
     3901 tacggtcttt tttgatggtt tcggtttcat cggcattgat cagttcgatc gctatccggt
     3961 gcacgaacga aacaaaatcc ggaatgtgcc ggccgaccgg ggtctggcgt ttgaactggc
     4021 cggcgaagcg gcggtcggtg gt
//
LOCUS       DASWGM010000496         6200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_887378,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000496 DASWGM010000000
VERSION     DASWGM010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887378"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..1974)
                     /locus_tag="VGA15_14200"
     CDS             complement(307..1974)
                     /locus_tag="VGA15_14200"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX9588882.1"
                     /translation="MNAPTAIPEPKPVPPYKHTPLFPLGPDTTPYKKITTEGVRVEKV
                     LGKDMLVVSREALRALSEAAFGDINHYLRPGHLQQLRNILDDKEASDNDKFVAFDFLK
                     NANIAAGGVLPMCQDTGTAIIMGKKGFRVLTDGDDEAALSEGARDAYLRRNLRYSQVA
                     PLSMYEEKNTANNMPAQCEIYAEGGSLDSDLAYKFMFMAKGGGSANKSFLFQATPSVL
                     TKDRMLAFLKEKVLTLGTAACPPYHLAIVIGGTSAELCMKTVKLASARYLDALPTHGS
                     ADGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRVIRLPRHGASLPIGLGVSCS
                     ADRQVLGKITKDGVYLEELEHNPAQYLPAVEQALGGEVVKIDLNQPMKDILATLSKHP
                     IKTRLSLSGTMIVARDSAHAKLRERLEMGEPLPDYFKNHPVYYAGPAKTPDGYASGAF
                     GPTTAGRMDSFVDQFQAAGGSMVMVAKGNRAVAVREACKKHGGFYLGSIGGAAANLAE
                     HCIKKVEVVEYPELGMEAIWRIEVEDFPAFIIIDDKGNDFFKELNLG"
     gene            2719..3273
                     /locus_tag="VGA15_14205"
     CDS             2719..3273
                     /locus_tag="VGA15_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588883.1"
                     /translation="MTKQQLATSISRLRPAGFGPAGFGSAGFGSARVAAALTLLATIS
                     ITALGASPARADRCDDLAAQLKSQIDGLGVGKTAANLIYLSHPQAKQITLGCAGRNVS
                     NQLFARADSRKPKPAFLELVASASAIIFTLPKDEMLRGATRCIQRMGLFRGDDVKTRY
                     RRLDMNCSRTKTDASITISRGTDQ"
     gene            3493..3663
                     /locus_tag="VGA15_14210"
     CDS             3493..3663
                     /locus_tag="VGA15_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588884.1"
                     /translation="MSISSVPPSLVVKAPEPVQWKAPKPKTDNNADTRGVQPPALAAL
                     PPGQGTRIDQLA"
     gene            3986..4345
                     /locus_tag="VGA15_14215"
     CDS             3986..4345
                     /locus_tag="VGA15_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX9588885.1"
                     /translation="MPDTKTYTGGCHCGLVRFECTTDLAMVTACNCSICTKKGLHFAF
                     LAPKSFQLRAGEDNLREYVFNRHAIRHQLCNDCGVDVFARGSKPDGTEVVALNVSCID
                     GIELAKLAMTPIDGRNR"
     gene            4796..>6200
                     /locus_tag="VGA15_14220"
     CDS             4796..>6200
                     /locus_tag="VGA15_14220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HEX9588886.1"
                     /translation="MASAALPTTLAHAGDATWNPGAVAPANADFNTGDNWAGTPDKTV
                     PTLGTATFNASNTQDVTITGTTNLGGMTLKSRAGTYTFTNTAGSTSPGLTFIGAGIIV
                     EDGAAAKIDNVSSNLNFAGTSSAGLATISNDDGSTIIFRGSSKAGHATITNLSGGKLL
                     FSGDSAQAPVSAQTATIINFSSSADSSGAEGFKFDNGSVAAAATIYNHGDLHFVGNSR
                     ANVALIQSDTGGTIFIEEHAKGDTARIVLGDGLTKGNLDISRLATLGTTVGSLAGSGN
                     VFLGSKNLDVFGFDQDTVFSGVIQDGTAAPLLGGGGSLTKGGTGALTLTGINTYTGTT
                     TVSGGALIVDGDGSIASSSVIVNAGGTLAGTGTVGSTISTTTINAGGTLAPGHGPGDG
                     TFGKLTVQGNLSFAAAATYLIQVSPTAASNTSVTGTASLNGATVKANFASGTYVKNEY
                     TILTAGTVDGQFNPLVTT"
BASE COUNT         1218 a         1982 c         1786 g         1214 t
ORIGIN      
        1 caatggcgac gacgtcgcct ggaaagtctc catcaccaac ccgatgccgc tgacgctgga
       61 attcaccggc aagatttccg gcgacaacat gaccggcgag atgggaatcg gcccgatggg
      121 cagctttccg ttcacgggga cgagggcgta ggttctatgc tcgtcgtccc ggcgaacgcc
      181 gggacccata atcccggacg gtaattgttg cggaaggcgt ctgcttcatt gccgaaacgc
      241 gactccgcgc cgtatgggtc ccggcgttcg ccgggacgac gtggagacgt ggtcggcgtt
      301 gccccctcac cccaaattca attccttgaa aaagtcattg cccttgtcgt cgatgatgat
      361 gaaggccgga aaatcctcga cctcgatgcg ccagatcgct tccatgccaa gctcgggata
      421 ttccaccacc tcgaccttct tgatgcagtg ctcggcgaga ttggccgcgg cgccgccgat
      481 cgagcccaga taaaatccgc cgtgcttctt gcaggcttcg cgcaccgcga ccgcgcggtt
      541 gccttttgcg accatcacca tcgatccgcc cgcggcctgg aactggtcga cgaaggaatc
      601 catccgtccc gccgtggtcg ggccgaacgc gcccgacgca tagccgtccg gcgtcttggc
      661 cgggcccgcg taatacaccg gatggttctt gaaatagtcc ggcagtggtt cgcccatctc
      721 cagccgctcg cgcaatttgg catgcgcgga atcgcgcgcg acgatcatgg tgcccgatag
      781 cgacagccgg gtcttgatcg gatgttttga gagcgtcgcc aaaatatcct tcatcggctg
      841 gttgagatcg atcttgacca cctcgccgcc gagcgcctgc tccaccgcgg gcagatattg
      901 cgccggattg tgttcgagct cttcgagata gacgccgtcc tttgtgatct tgccgagcac
      961 ctggcggtcg gccgagcacg acacgcccaa gccaatcggc aacgaggcgc catggcgcgg
     1021 cagccggatc acccgcacgt cgtgacaaaa atatttgccg ccgaactgcg cgccgacgcc
     1081 gagcgactgc gtcatcttga ggatttcctg ctccatctcg agatcgcgaa aagcgttgcc
     1141 gtcggccgag ccgtgggtcg gcagcgcgtc gagatagcgt gccgaggcca gttttacggt
     1201 tttcatgcag agctcggccg aggtaccgcc gatcacgatg gcgaggtgat agggcgggca
     1261 cgccgcggtg ccgagcgtca atactttctc cttcaaaaac gccagcatgc gatcctttgt
     1321 cagcaccgac ggcgtcgcct gaaacaagaa gctcttgttg gccgacccgc cgcccttggc
     1381 catgaacatg aacttgtagg ccaagtctga atctaaagag ccgccctcgg catagatctc
     1441 gcactgcgcc ggcatgttgt tggcggtgtt tttctcctcg tacatcgaca gcggcgccac
     1501 ctgcgaataa cgcaaattgc ggcgcagata ggcgtcgcgc gcgccctcgc tcaaggcggc
     1561 ctcgtcgtcg ccatcggtga gcacgcgaaa acccttcttg cccatgatga tggcggtgcc
     1621 ggtatcctgg cacatcggta acacgccgcc ggcggcgatg ttggcgttct tcaggaaatc
     1681 gaaggcgacg aacttgtcgt tgtcgctggc ctccttgtca tcgagaatgt tgcgcagctg
     1741 ctggagatgc cccggccgca gataatgatt gatgtcgccg aacgcagcct ccgacagcgc
     1801 ccgcagcgcc tcccgcgaca ccaccagcat gtccttgccc aacacctttt cgacccgcac
     1861 gccttcggtg gtgatcttct tgtacggcgt ggtgtccggc ccaagcggaa acagcggcgt
     1921 gtgcttgtag ggcgggacgg gttttggctc gggaatggcg gtgggggcgt tcatgggttt
     1981 tctcgattgt agtgggttta tgagcgtggc gggaacagta aaacactaag atatgccagg
     2041 cgacttgctt tgctgcgata caccaaacca tatcggcttg tgtcgcgatt tgttacacca
     2101 atgaactgat ccgcagcagc agtgatggtt gcgtggaacg tcgcatagta agctgccgaa
     2161 attgctcgtc taatatccac ctggcgcggg ggtccaacct gaatagcaat gagcttgttg
     2221 gcctgctcaa atagatgact aggattaaga accgccactt gcgttgagct ctttcttcgt
     2281 agcgtactcc actattggga aacgttcttc gccttccgcg cgaagccgac cctgtatccc
     2341 aacaagagtg tccaacgtcg cgtcgccctt aatcctcgac gctgagccgg gctttatcac
     2401 gatggtaatt cggagcgcat cgcgcccctc agagtcgaca gcggcgctac tagaaaccga
     2461 agacacattt gcactggtca gattagcagt cgcaacttct tgcgcaatct cacgtattct
     2521 tttctcatct aacatcgtgc ggccaatcag ttagaacggt ttcagcgtga ctgtagcacc
     2581 ttgagtgtgc tgtgcccaga cgcacacaga ttgattgctc ctgccgtgat cgccgatacg
     2641 gtcacggcac gtattgacgc ggtttgacgg accttgcatg aacctcccgc gcgcgtttca
     2701 atggcgcggg gggtatccat gacaaaacag cagcttgcaa cgtcgatctc aaggcttagg
     2761 cccgcaggtt ttgggcccgc aggttttggg tcggctggtt ttgggtccgc gcgcgtcgcc
     2821 gccgcgctga cgctgctggc tacaatttcc atcaccgcgc tcggcgcctc gccggcgcgg
     2881 gccgaccgct gcgacgatct cgcggcccag ctcaaaagcc agatcgacgg cctcggcgtc
     2941 ggcaagaccg ccgcaaattt gatctatctg tcgcacccgc aggccaagca gatcacgctc
     3001 ggctgtgccg gccgcaacgt cagcaaccag ttgttcgccc gggccgacag ccgcaagccg
     3061 aagccggcct ttctcgagct ggtcgccagc gcttccgcca tcatcttcac cttgccgaag
     3121 gacgagatgc tgcgaggcgc gacgcgctgc atccagcgga tgggcctgtt ccgcggtgac
     3181 gacgtcaaga cccgctaccg ccgcctcgat atgaattgca gccgcaccaa aaccgacgca
     3241 tcgattacga tctcgcgcgg cacggaccag taaccggacg cgtttcggtt aacggagctg
     3301 ctgcaattcc cggagaattg gtcgttttcc ctgagcttcc ggttcaccgt gaggcgggaa
     3361 tagccttgat ggcgcgggat cgacggatcg gattcataaa gcattcgtcc ccgccccgtc
     3421 acagtcgggc acaacgaagc ccaaggctat tgtctgaagg ctgaaggctg ttgtctgaca
     3481 aaggatattc ccatgagtat ttcgagtgtc ccaccgtcgc tcgtcgtgaa agcgcctgag
     3541 cccgttcagt ggaaggcgcc gaagccaaag accgacaaca atgccgacac ccgcggtgtc
     3601 cagccgccgg ctctggccgc cctgcccccg ggccagggaa cgcggatcga ccagctcgcc
     3661 tgagggcggg ctgaattctg ctgatcgccc tccaagcttg catctgtcgt cgcccggctt
     3721 gaccgggcga tccagtatcc cagagcgttt agactgatta ctaacggcgg cgattactgg
     3781 atacccgcct tcgcgggtat gacggccaat tgtggatcag cgcactgcgc ccttgcttat
     3841 cccttgagaa ttatcccttg agaaacactc gccgcgggtt tgccgaagag gctcgccccc
     3901 cacccggatt ttggtgagga tcattttctc gcgaaatcgc ttatagtctg atcgtggtga
     3961 taaccgtccc tcgtggagcg gaccgatgcc tgacacaaaa acctataccg gcggctgcca
     4021 ttgcggcctg gtgcgcttcg aatgcaccac cgatcttgcg atggtgaccg cctgcaactg
     4081 ctcgatctgc accaagaagg gcctgcattt cgcgttcctg gcgccgaaga gttttcagct
     4141 ccgcgccggc gaggacaatc tcagggaata tgtgttcaac aggcacgcga tccggcatca
     4201 gctctgcaac gactgcggcg tcgacgtgtt cgcgcgcggc agcaagcccg acggcaccga
     4261 agtggtggcg ctcaacgtca gctgcatcga cggcatcgaa ttggcaaagc ttgcgatgac
     4321 gccgatcgat ggaagaaatc ggtaaccgtc attccgggat gcgccacttg gcgcaggccc
     4381 ggaatccata ctccctgcag gggttatgga ttccgggctc gctcgttcct cgcgccccgg
     4441 aatgacgaag aggtgcgtta aacatccagc gccccgtagc ggcgaaaaat gccgtattca
     4501 ttgaacgcga cgcgactgtc gcttgcagca tagtccgcga cacgcggcgg ctgtcgaggc
     4561 ttccatgctt cccccaatcg cgtgaagcga aaaatcaaca ctctcgatca aaccgacggt
     4621 ttcccccgtt cgccggaagc gcgccgcgcc ccgcaacggt aacgaggtga cgaaacacct
     4681 tgaaggggcc accatgcgac cgcaagcttc acattcatcc catcaaaccg ccagccgccg
     4741 cgccgcgccc cgccgggcgt cacgcctgcg aatcatgctg ctcgcgagct cggcgttggc
     4801 gtccgcggcg cttcccacga cgcttgccca tgccggcgac gcgacatgga acccaggcgc
     4861 agtcgccccg gcgaacgccg acttcaacac tggtgataat tgggccggca caccggataa
     4921 gacggtcccc accctcggga ccgcgacgtt caatgccagc aacacccagg atgtcacgat
     4981 cactgggaca accaatctcg gcggaatgac gctgaagtcg cgcgccggaa cgtatacgtt
     5041 cacgaatacg gctggtagca cgtccccggg tctcacgttc attggcgccg gcatcattgt
     5101 cgaagacggc gccgctgcga aaattgacaa cgtctcttct aacctcaact tcgccggcac
     5161 aagttcggcg ggcctggcga caatcagcaa cgacgatggc agtactataa tcttccgcgg
     5221 cagcagcaaa gcgggccatg cgacgatcac aaacctttcc ggtggcaaac tgctgttttc
     5281 tggtgattcg gcgcaagctc ctgtttcggc gcaaactgct acgatcatca acttttcgag
     5341 tagcgccgat tcgagtggcg ccgagggttt taaatttgac aacggcagcg tcgccgccgc
     5401 cgcgacaatc tacaatcacg gcgaccttca tttcgtcggc aacagtaggg ccaacgttgc
     5461 cctcatccaa tccgataccg gtggcaccat ctttatcgag gagcatgcaa agggcgacac
     5521 cgcccgcatc gtgctcggcg atggcctcac caaaggcaat ctcgatattt ccagactcgc
     5581 gacattgggg acgacggtcg gttcgctcgc aggcagcggc aacgtcttcc tcggcagcaa
     5641 gaaccttgac gttttcggct tcgatcagga taccgtattt tctggtgtca ttcaggacgg
     5701 caccgccgcc cccttgttgg gcggcggcgg ctcgctgacc aaggggggca ccggtgcgct
     5761 gaccctcacc ggcatcaaca cctataccgg cacaaccacc gtcagtggcg gcgcgctgat
     5821 cgtcgacggc gacggctcga tcgcgtcctc gagcgtgatc gtcaacgccg gcggcaccct
     5881 cgccggaacc ggcaccgtcg gcagcacgat ctccaccacg acgatcaacg ctggcggcac
     5941 gctggcgccc ggacacggcc ctggcgacgg cactttcggt aagctgacgg ttcagggcaa
     6001 tctctcgttc gccgcggcgg cgacctatct catacaggtc tcgcccacag ccgcctccaa
     6061 caccagcgtg acgggaacgg cttcgctcaa cggcgccacc gtgaaggcca acttcgcgtc
     6121 cggcacctat gtgaagaacg aatacaccat cctcactgcc ggcaccgtcg acggtcagtt
     6181 caaccccctg gtcaccacca
//
LOCUS       DASWGM010000497         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2669355,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000497 DASWGM010000000
VERSION     DASWGM010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2669355"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VGA15_14225"
     CDS             complement(<1..687)
                     /locus_tag="VGA15_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588887.1"
                     /translation="MFITLAGILAVVGTLAGGYYFAMRPVTLRIAVGPTNSDDIKVVQ
                     ALTQAFSHAHGQVRLHPTQTDGAAASAQALAEGKADLAIIRGDLDVPKNAQAVATLRK
                     NVAVLWVPPAAKGKGKKAGSKITKIAQLSGHRIGVIGRTPANVNLLKVILQQYGVDPN
                     KVDIVQFPAGESADAIRNQKADAYLAAGPVNSKITADAIAASTRDGGTPTFLAIDSAE
                     AIAQNHPMYES"
     gene            931..1254
                     /locus_tag="VGA15_14230"
     CDS             931..1254
                     /locus_tag="VGA15_14230"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX9588888.1"
                     /translation="MKQEAIMAERLSAEARKSALQGLPGWTETPGREAIARNFAFKDF
                     NEAFGFMCRAALVAEKNDHHPEWRNVYKTVEVVLATHDAGGVTALDIDLAKAMNTIAK
                     QLGVA"
     gene            1356..1739
                     /locus_tag="VGA15_14235"
     CDS             1356..1739
                     /locus_tag="VGA15_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HEX9588889.1"
                     /translation="MASEHTVGFEPADRLAKDRESVRRRFWIKLKRVVARLPFAEDLL
                     AAYYCAFDKQTPRHVQAALLGAIAYFILPFDFIPDMLPVLGFTDDAAVLATAIRMVAT
                     HITPEHREAARAALKRGIETAEPSE"
     gene            complement(1809..>2397)
                     /locus_tag="VGA15_14240"
     CDS             complement(1809..>2397)
                     /locus_tag="VGA15_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588890.1"
                     /translation="GRFHLVTVDYDRPLQGDYLPATMTSKDYPNLIKEGESVDTIAVP
                     AVLAAYNWAPNTDRYRKLSLFVDAFFTKFPTLQNPPFHPKWKEVSLSAPLPGWQRLPS
                     AKQWLDQHGIEPVARNRFEEFPAQTAAPAAGVRPAAAAAGNLRQAPAAAAPAPAAAAR
                     PQTAAEQEALFKQFQAWEAERNASAQARPAPQLPR"
BASE COUNT          426 a          726 c          789 g          456 t
ORIGIN      
        1 ggattcgtac atcgggtggt tttgcgcgat cgcctcggcc gaatcgatcg cgagaaaggt
       61 cggcgtgccg ccgtcgcgtg tcgatgccgc gatcgcatcg gcggtaatct tgctgttgac
      121 cggtccggcg gcgagatagg catccgcctt ctggttgcgg atggcgtccg cggattcgcc
      181 agccgggaac tgaacgatat cgaccttgtt cggatccaca ccgtattgct gcaggatcac
      241 cttgagcaga ttgacattgg ccggggtgcg cccgatcacg ccgatccggt gtccggagag
      301 ctgcgcaatc ttcgtgatct tcgaaccggc tttcttgcct ttgcccttgg cggccggagg
      361 aacccatagc accgccacgt tcttgcgcag cgttgccacg gcctgggcat ttttcggcac
      421 atcgagatcg ccgcggatga tcgcgagatc ggccttgcct tcggcaagtg cctgggcgct
      481 cgcggcggcg ccgtcggtct gcgtcgggtg caaacggacc tgaccgtggg catgggaaaa
      541 ggcctgggtc agcgcctgga ccactttgat gtcatcgctg ttggttggac cgactgcaat
      601 ccgcagggtc accggccgca tcgcaaaata gtaaccgcct gcgagcgtgc ccaccacggc
      661 gaggatcccc gccagggtga tgaacatcat cttctgcttc gccgaacgcg gcgacggcag
      721 ggtcggcccg acaaggtcga ggcccccggt catcgatctc gcaaacaagc gtttgatgct
      781 caatttcatc ttgccaatgc ggttagaccg gcgttgtagc aaatttccct tcggcgcagg
      841 gttacatctg cgtcatggtt agcggcaggc tttgccgcct cgatgtagtg tggatcccag
      901 gggggcggtt ggtgttaggg taaagcaggt gtgaaacagg aggcgatcat ggcggaacgg
      961 ctgtcggcgg aggcgcgaaa atcggcgctg caggggttgc cagggtggac cgagacgccc
     1021 ggacgcgagg caattgcgcg gaactttgcc ttcaaggatt tcaacgaagc tttcgggttc
     1081 atgtgccgcg ccgcgttagt ggctgaaaag aacgatcatc accccgaatg gcgcaatgtc
     1141 tacaagacgg tggaggtggt tctggcgacc catgacgccg gcggcgtcac cgccctcgac
     1201 atcgatcttg caaaggcgat gaataccatc gcaaagcagc tcggcgtcgc ctgattggca
     1261 acgcacaatc ttgcggcggc ggcgccacat ccccaactct ggaggcggca gcctagctag
     1321 gcggctgacc gcgcatctcg agggagcctt aagtgatggc atccgaacat accgtgggat
     1381 ttgagccggc cgacaggctg gcgaaagacc gcgaaagcgt gcgcaggcgc ttctggatca
     1441 agctgaagcg ggtcgtcgcc aggctcccgt tcgccgagga tttgctggcg gcctattatt
     1501 gcgcgttcga caagcagaca ccgcgccatg tgcaggcggc gctgctcggc gccatcgcct
     1561 atttcatcct gccgttcgat ttcatcccgg acatgctgcc ggtactcggc ttcaccgacg
     1621 acgccgcggt actggcgacc gcgatccgga tggtcgccac ccatatcacg ccggagcatc
     1681 gcgaagccgc ccgcgccgcg ttgaagcggg ggatcgagac ggctgaacca tcggagtgac
     1741 ggtggttcat cgagagcgat cggcgagccg gaatattcct ccgactcggg atcactcccg
     1801 cataaaacct atcgcggcag ttgcggcgcg gggcgagcct gcgccgaggc attccgctcg
     1861 gcttcccatg cctggaattg cttgaacaag gcttcctgct cggccgccgt ctgcggcctt
     1921 gccgccgcag caggggcggg ggctgcggcc gctggggcct gcctcagatt tcccgctgcg
     1981 gcagcggccg gcctaacgcc tgccgcagga gcggcggtct gcgccggaaa ttcctcgaac
     2041 cggttccgcg cgacgggctc gatgccgtgc tgatccagcc actgcttggc gctgggcaag
     2101 cgctgccaac cgggaagcgg agccgacagc gacacctcct tccacttcgg atggaagggc
     2161 ggattctgga gggtcgggaa cttcgtgaag aaagcatcca cgaacaggga gagcttccga
     2221 tagcggtcgg tgttgggtgc ccaattatag gcggctagca ccgcgggaac cgcgatggtg
     2281 tcaaccgatt ccccttcctt gatcaggttc ggataatcct tcgacgtcat ggtcgcgggc
     2341 agatagtcgc cctgcagcgg cctgtcgtaa tcgaccgtaa ccaggtggaa ccggcct
//
LOCUS       DASWGM010000498         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_452361,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000498 DASWGM010000000
VERSION     DASWGM010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452361"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VGA15_14245"
     CDS             <1..1527
                     /locus_tag="VGA15_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588891.1"
                     /translation="GRTPPPTTSVSRRYAALALPLAPAWIWSAYAALFPGQELLGTTL
                     YWLFWAGTGIPLLIAALLAADSIIRRRDAFYDRFAGTRVLRLDRKNAVVAMAAVAPVP
                     SLTGSVPSDPELAPQASTALPPPLPPQALRSRNYLVRHWRGELSLPVSYWLNGTVSGL
                     VVGLVIGGMAYLINWQGDAEPVVWLSTLIASWIFAALLTIWQSVGIWRAAIRYRQSGK
                     DFWGGVAQMLTVAGLLVFPYDFATVGAPQLAGIYEIVSGDARVGPHEFHVLANGQTVE
                     FSGGITFGVTQELERFLDAMAGVRTVRLNSNGGRILEAQRMSDLISSRNLATFVARDC
                     LSACTIVFLGGKERFMLPTARLGFHQPAFRGMTASDRRAAIATEQRRLQQRFGLSAAF
                     AARANSAPPGGMWYPDKDELMRERVVTRLVSPKQVLPSPTDAPTANAAPSSADGEHAT
                     PVARAPVPAIRIPGTGEPTASVGAAADGAVRAKIPAELLKRLAAQPRKPVAIPPAAAR
                     "
     gene            complement(1728..2036)
                     /gene="rpmB"
                     /locus_tag="VGA15_14250"
     CDS             complement(1728..2036)
                     /gene="rpmB"
                     /locus_tag="VGA15_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX9588892.1"
                     /translation="MSRRCELTAKGPQVGHKVSHSNIKTKRRFLPNLCNVTFISDALG
                     RNVRLRVSTNAIKSVDHRGGFDAFLIKAKAAELSPRALELKRQIEKKTAATAPAAKAN
                     "
     gene            2376..3242
                     /locus_tag="VGA15_14255"
     CDS             2376..3242
                     /locus_tag="VGA15_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX9588893.1"
                     /translation="MPTNRMISRRLVPLALRVLGGLCTGACLLLAAPSAFAQGKLDAR
                     YEATLAGIPVGKGAWTIDIADDSFSAAASGGTSGLLKAFAGGSGTGAAQGRVVNGALV
                     SSNYSATTTTSKKSEAIRIVLSNGNVKDYAIEPEPPVDPDRLPITDAHRRGVYDPMTG
                     SMLRVPGSGDPLSPEACRTGAAVFDGRMRYDLKLDFKRMETVKAEKGYHGPVVVCAVY
                     FSPVAGYIPDRPVIKYLAAQRNIEITFAPIAGTRILVPFRMVIPTPLGTAMLEATQFI
                     TQASPPRVAKTQ"
     gene            3649..>4232
                     /locus_tag="VGA15_14260"
     CDS             3649..>4232
                     /locus_tag="VGA15_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEX9588894.1"
                     /translation="MAFSSSAPAFANGRTPGAGVTAVLGPTNTGKTHLAIERMLAHSS
                     GVIGLPLRLLAREVYNKIVDRAGVDAVALITGEEKIKPPKARFWVSTVEAMPRDLDVS
                     FLAVDEIKIAADLERGHVFTDRILHRRGRDETLLLGAATMRPIIERLLPGASIVTRPR
                     LSQLEFAGDRKITRQPRRTAIVAFSADEVYAIAEL"
BASE COUNT          728 a         1383 c         1326 g          795 t
ORIGIN      
        1 ggacgcaccc cgccgccaac gacgtcggtg agccggcgct acgcggcgct ggcgttgccg
       61 ctggcgccgg cctggatctg gtcggcctat gcggcactgt ttcccggcca ggagctgctc
      121 ggcaccacgc tgtactggct gttctgggcc gggaccggca tccccttgct gatcgctgca
      181 cttctcgcgg cggattcgat catccgtcgc agggacgcct tctacgatcg cttcgcgggc
      241 acgcgtgtgc tgcgcctcga caggaaaaac gccgttgttg cgatggccgc cgtggcgccg
      301 gtgccgtctc tcaccggaag cgtcccgtcc gatccggaac ttgcgccgca agcgtccacc
      361 gcgctgccgc cgccattgcc gccgcaggcg ttacgatcgc gcaattatct cgtgcggcac
      421 tggcgcggcg agttgtcgct gccggtgtcg tattggctga acggaaccgt gagcggtctg
      481 gtcgtcggtt tggtgatcgg cggcatggcc tacttgatca actggcaggg cgacgccgag
      541 ccggtggtct ggctgtccac gctgatcgcg agctggatct tcgccgcgct gctgacgatc
      601 tggcagtcgg tcggaatctg gcgcgccgcg atccgctacc ggcaaagcgg caaggatttt
      661 tggggcgggg tcgcgcaaat gctgaccgtg gccggattgc tggtgtttcc ctatgatttc
      721 gcgaccgtag gcgcgccgca actggccggc atttacgaaa tcgtcagcgg cgatgcccgt
      781 gtcggcccac atgaatttca cgtcctcgcc aacgggcaaa ccgtggaatt ttccggtggc
      841 atcaccttcg gcgtgacgca ggagcttgaa cgctttctcg atgcgatggc gggcgtccgc
      901 accgtgcggc ttaattccaa cggcggccgt atcctcgaag cccagaggat gtcggatctg
      961 atcagcagcc ggaatctggc gaccttcgtc gccagagact gcctgtcggc ctgcaccatc
     1021 gtgttcctcg gcggcaaaga gcgtttcatg cttcctaccg cgcggctcgg tttccatcag
     1081 ccggcgtttc gcggcatgac ggcatccgac cgccgcgccg cgatcgcgac cgaacagcgg
     1141 cggctgcagc agcggttcgg attgagcgcg gccttcgcgg cgcgggccaa cagcgccccg
     1201 ccaggcggca tgtggtatcc cgacaaggat gaattgatgc gggagcgcgt ggtgacgcgg
     1261 ctggtctcgc ccaaacaggt tttgccaagc ccgaccgacg caccaactgc caacgccgcg
     1321 ccgtcgtctg ccgatggcga acacgccacg ccggtcgccc gcgcgccagt gccggcgatc
     1381 cgcattcccg gcaccggcga gccgaccgcg tccgtgggtg ccgcggccga cggagccgtg
     1441 cgggccaaaa tcccggcgga gttgctaaag cgcctcgccg cgcagccgcg caagccggtg
     1501 gcgattccgc cggcggcggc gaggtagggg aggggcgaga gagcgcaata cgcctgcgtc
     1561 attctgaagg ggcgcgctcg gggtcttgaa atctcacgac ctcagccact cgcacggtcg
     1621 atccctcggt agcgcgcgag cgcaagatag aacttcaaaa caaaacagcc gggtctttcg
     1681 acccggccat tcaaaactct tcagcattga cccgttggac gaaagcctca gttcgccttc
     1741 gccgcgggtg ctgtcgctgc ggtcttcttc tcgatctggc gcttcagctc cagcgcgcgg
     1801 ggcgacagct cggctgcctt ggccttgatc aggaatgcgt cgaagccgcc gcggtgatcg
     1861 acggacttga tcgcgttggt cgaaacccgc aaccgcacgt tccgtcccag cgcgtcggaa
     1921 atgaaggtga cgttgcacag gttcggcagg aaccggcgct tggtcttgat attggagtgg
     1981 ctcaccttgt ggcccacctg cgggccctta gccgtcagtt cacagcgccg cgacatcgcg
     2041 aaatcctctt ccgtccccag gctcgacgcc tcggggttca taaattacgt ccatccaggg
     2101 aaccgcggac gtataggggg agagcggcaa ggcgtcaagg aaggcgttaa gcttttgcgt
     2161 cgccccggag ccccgcgaaa aggcggtttc gcaaggagtt ccatcggctt aagacatcat
     2221 ataaacggcg caatcgccgc cgaaatgatg ctcttgtgct ggctcccacg gtaagcttgc
     2281 ggattcgcgg aattaaacac acgccaattc gttgtttgcc gggtattttc gcttaaatga
     2341 cagatcgatc gcgcctacct gaggaccgcc gttcagtgcc cacaaatcga atgatttcgc
     2401 gccggctggt cccattggcg ctgcgggtgc taggcggcct ttgcaccggc gcatgcctgc
     2461 tgctggcggc gccgtccgcc tttgcccagg gcaagctcga tgcgcgttac gaggcaacgt
     2521 tggccggcat tcccgtcggc aagggcgcct ggaccatcga catagcggac gactcgttct
     2581 ccgccgcggc gtcgggtggc acctcgggac tattgaaggc gttcgccggc ggttccggca
     2641 ccggcgcggc acaaggccgc gtcgtcaacg gcgcgctggt gtcgagcaat tattccgcca
     2701 ccaccacgac gtcgaaaaaa tccgaagcca tccggatcgt tttgtccaac ggcaacgtca
     2761 aggactacgc catcgagccg gaaccgccgg tcgatcccga ccggcttccc atcaccgacg
     2821 cgcatcgccg cggcgtctac gatccgatga ccggatcgat gctgcgggtg ccggggtcag
     2881 gcgatccgct cagccccgag gcctgccgca ccggggctgc ggtcttcgac gggcgcatgc
     2941 gctacgatct caagctcgac ttcaaacgca tggaaacggt gaaggccgaa aagggctatc
     3001 atggcccggt ggtggtctgc gcggtctatt tctcgccggt cgcaggctac atccccgacc
     3061 gccccgtaat taaatacctc gctgcccagc gcaacatcga aatcacgttt gcgccgatcg
     3121 ccggcacccg gatcctggtt ccgttccgga tggtaatccc gacgccgctc ggcaccgcca
     3181 tgctggaggc cacccaattc atcacccagg cgtcgccgcc gcgcgttgcc aagacgcagt
     3241 gatgcgtttt tccgtcatgg ccgggcggaa gcgcgtcttc gcgctagatg tcctggccat
     3301 ccacgtcttt ctcgctgcta tgcgtttgcg caaaacagac ggctccggcc atgcgtgacg
     3361 agtgctttga aagcgtggaa tcctgttgac tcctgacgca attgtcctat tcgcgcaatt
     3421 aaggatttcg cagccaaacc gccgcttgtc gcgccccgcc aaacttgatc tagtgccgcg
     3481 ataaggtgcc aacgcgagat gttgcggtga acgaagccgg agttggtgcg agtcagcgat
     3541 tcgggcgcga ttcgttccgg actcgttcca gagtttaagc cgcgaccgca atacgtcgca
     3601 ttgtgaaaca cttctccaac gcagcgacgc agaaacttct tacaaaacat ggccttttct
     3661 tcttcagccc ccgcgttcgc caacggacgg actcccggcg ccggcgttac cgcggtgctc
     3721 gggcccacca acaccggcaa gacccatctg gcgatcgagc ggatgctcgc acattcctca
     3781 ggcgtgatcg ggttgccgct gcggctcctg gcgcgcgagg tctacaacaa gatcgtcgat
     3841 cgtgccggtg tggacgccgt ggcgctcatt accggcgaag aaaagataaa accgccgaag
     3901 gcacggttct gggtctccac cgtggaggca atgccgaggg atctcgacgt gtcgtttctc
     3961 gcagtcgatg aaatcaagat cgcggccgat ctggaacgcg ggcacgtctt caccgatcgc
     4021 atcctgcatc ggcgcggccg cgacgaaacg ctgctgctgg gtgctgccac catgcgcccg
     4081 atcatcgagc gactattgcc cggcgccagc atcgtcaccc ggccgcggct ctcgcagctt
     4141 gaattcgccg gcgatcgcaa gatcacgcgg cagccgcggc gcaccgcgat cgtggcgttt
     4201 tcggccgatg aagtttatgc catcgccgaa ct
//
LOCUS       DASWGM010000499         5169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2275011,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000499 DASWGM010000000
VERSION     DASWGM010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275011"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VGA15_14265"
     CDS             complement(<1..620)
                     /locus_tag="VGA15_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9588895.1"
                     /translation="MRFDLVDLQLFVAVADARSITGGALRAHLALASASARIKGLEAA
                     LGTALLKRGRRGIELTAAGESLLDHARIILHHVETMRGDLSAYSSGARASVHLLANTS
                     GLSEHLPKALAAFLREHPDINVDIEERESTDIAAAIATGAADLGFAAEHALPDNVERF
                     LFSEDRLMLVTARRGDLAGRRQIDFQEVRDRDFVGLTNSTALQIHIA"
     gene            715..1473
                     /locus_tag="VGA15_14270"
     CDS             715..1473
                     /locus_tag="VGA15_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9588896.1"
                     /translation="MFEPLLFLVAAVFLLAGFIKGVIGLGLPTVSMGLLAVTMQPSHA
                     LAIVIVPAVVTNIWQTFVGPYLRDIIRRLWPLMAGTVIGIWLNAGMLTGPYARYGAIV
                     LGVLLVIYAVIGLSAFSFSVARRDEKWIGGIVGVVTGMVSAATGVQVIPSMPYIQAIG
                     MEKDELVQALGVFFTVATLGLAFNLTSAGLLTAATALPGAIAMAASFTGMFIGQAVRS
                     RLQPETFRRWFLIAMIFLGLYLAGSALYNVIQRA"
     gene            1728..1982
                     /locus_tag="VGA15_14275"
     CDS             1728..1982
                     /locus_tag="VGA15_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588897.1"
                     /translation="MKKSFWRLGCAAAFAFLLVQSSLAQKLVDPDSVAPAFRAAAEKR
                     RAEQLKLNDCTKKANVAKILVRDRAAFITTCLDSTNETAN"
     gene            2100..2543
                     /locus_tag="VGA15_14280"
     CDS             2100..2543
                     /locus_tag="VGA15_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588898.1"
                     /translation="MAEDDNSDDYKVTLAQQIFGRAAVISGRNPPELRTNDAPEAKIP
                     PVAARDSPVREVLSATLEPGEAVAQQRQLKTAAELAELIESDLARHPDCPKRGFRVTV
                     YGGSHWRAMLTITPAAGPVRNPGEWRDLTNDLAERLRKHYDLAWE"
     gene            2742..3872
                     /locus_tag="VGA15_14285"
     CDS             2742..3872
                     /locus_tag="VGA15_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX9588899.1"
                     /translation="MTDAELSIIDEIKTTIHIGSAEKRLATAKHVTDLFLSSAGSFNH
                     EQIELFGDLLERLIKTIEIRAIADVSARIALAEMSVQLAPVAQAPPSVVRRLAMNDEI
                     AVAGPVLTESARLSTEDLVEIAETKGEQHLLAISGRWWLKEVITDALLARRYPTVSRR
                     IVSNPGARVSAAGFAIVVAQAESDPELAIETGIRVDLPSALRRQLLRNATEAVRSRLL
                     SRAPPHIFEEIRSAIATVAAGADREMSNVRDFTAAKRLVARLKNNAELNEATLFGFAK
                     QRKYEETVAALAELSRSTIEIIRPLMQSLRDDGVLFPCKAAELSWETVSAVLDSRFAT
                     GSMAPHELAKAKAQFAKITPENARRLLRFWQVRSSPSPSRVN"
     gene            4054..>5169
                     /locus_tag="VGA15_14290"
     CDS             4054..>5169
                     /locus_tag="VGA15_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX9588900.1"
                     /translation="MTEAKSLLAELEDAVSRGTAESCLQALWHATDILIAGRYSEEQI
                     WTFGEIIGRLAEDIEIAARVRLANKLAPSSNAPVKVIKQLAFDDSIDVAGPVLRQSGR
                     IDANTLVANARSKSQQHLLAISKRKSIPPEVTDVLVVRGTAEVATSVAANDGARLSGS
                     GFLHLVKRSEDDGILAEHLGLRKDIPRHVFQQLISKASDEVKKKLERERPEMGSQIQA
                     AVTDVTGTIHAKFGPASKNYFAAKRIVGKLHEYGSLNEDKIFEFAHSLKFNETAVALS
                     LLCVLPIDVVERALVDKNREVILILARALDLSWTTTMSLLFLGAANYRITAGDLDRMK
                     REFARLNVETSRQVLKVYQSRKETAADSSGLRPLPQLH"
BASE COUNT         1038 a         1588 c         1500 g         1043 t
ORIGIN      
        1 gcgatatgga tctgaagcgc ggtggaattg gtcagtccga cgaagtcgcg atccctcacc
       61 tcctgaaaat cgatttgacg acggccggca agatcgccgc gccgcgcggt gaccagcatc
      121 aagcggtcct cgctgaacag aaatcgctcg acattgtcgg gcaaagcgtg ctcggcggca
      181 aagccgagat ccgcggcccc ggtggcgatc gccgcggcga tatcggtgct ttcacgctcc
      241 tcgatatcga cgttgatatc gggatgttcg cgcaagaagg cggcgagcgc cttcggcaga
      301 tgctccgaca ggcccgaggt gttggcgagc aaatgcacgc tggccctggc gccgcttgag
      361 taggccgaaa gatcgccgcg catggtttcc acatggtgga gaatgatgcg ggcatgatcg
      421 agcagacttt cgcctgccgc ggtcagctcg atgccgcggc ggcctcgctt cagcagcgcg
      481 gtgccgagcg ccgcttcgag ccccttgatc cgcgcgcttg ccgacgccag cgcgagatgg
      541 gcgcggagcg cgccaccggt gatgctgcgc gcatccgcca ccgcgacgaa cagctggagg
      601 tcgaccagat cgaaacgcat ggcaggcccg caaatctttg ccttcttcgg atccgaaggc
      661 ttgctccgta agctgcagat tgtgccgatc acgccgatcg gtcaatgtga ccagatgttc
      721 gaacctctgc tctttctcgt cgccgccgtc ttcctgctcg cgggtttcat caagggcgtg
      781 atcgggcttg gcctgccgac ggtgtcgatg ggcctgctcg ccgtcacgat gcagccttcg
      841 cacgcgcttg ccatcgtcat cgtgccggcg gtcgtcacca atatctggca gactttcgtc
      901 ggcccgtact tgcgcgacat catccggcgg ctgtggccgt tgatggcggg cacggtgatc
      961 ggcatctggc tgaacgccgg catgttgacc ggcccctatg cgcgctacgg cgccatcgtt
     1021 ctcggcgtcc tgctggtgat ctatgccgtc atcggcttaa gcgcgttcag cttcagcgtc
     1081 gcgcgccggg acgaaaaatg gatcggcggc atcgtcggcg tcgtcaccgg catggtttcc
     1141 gccgcgaccg gggtgcaggt gatcccgtcg atgccttata ttcaggcgat cggcatggag
     1201 aaggacgaac tggtgcaggc gctcggggta ttcttcaccg tcgcgacgct ggggttggcc
     1261 ttcaatctca ccagcgccgg gttgctgacc gccgcgaccg cgctgcccgg cgccatcgcc
     1321 atggcggctt ccttcaccgg catgttcatc ggccaggcgg tgcgctcgcg cctgcagccg
     1381 gagaccttcc gccgctggtt tctgatcgcg atgatttttc tcgggctcta tcttgccggc
     1441 agcgcgctct acaatgtcat tcagcgcgcc tgaggcatgg cgacgcgggg cgccgagata
     1501 ggcgaacagt ccgcccagtg cgccattgat ccagcgcaag ggattggccg gatcgcctga
     1561 tgctgccggc ggccccggcg gcgaacgcgg ccacgtctag gcgaagcggc gtcgccgtgc
     1621 aattgtggac tggttcacat ccgccgtttg acatttaagc tagaaggctc caatcgaacg
     1681 acattttccc ggctaccccc tttttccggc tacccctgga gccgaacatg aagaaatcct
     1741 tttggcgtct cggctgtgct gcggcgttcg catttctctt ggtccaaagc agtcttgcac
     1801 agaagcttgt cgaccccgat tccgtcgctc ccgcatttcg cgccgccgcc gaaaaacggc
     1861 gcgcggaaca actcaagctg aatgattgca ccaagaaggc caatgtggcg aaaattctgg
     1921 tcagggaccg agccgcgttt atcaccacct gcctcgacag cacgaacgag acagcaaact
     1981 agccgcgagg aacttttacc gctcaggctc ccgcttggcc ctgccctccg ctgcggcgtg
     2041 gcttcggcca gtgctttcat gctagaaaac gacaattgat ggatgagcgg gatacgaagg
     2101 tggcggaaga cgacaacagc gacgattaca aagtcactct ggcgcagcaa atcttcggaa
     2161 gagcagcggt aatctccggg cgcaatccgc cggagctcag aacgaacgat gcgccggagg
     2221 ccaaaatacc tcctgttgcc gcgcgcgatt cccccgtccg tgaagttttg tcagcgaccc
     2281 tggagcctgg cgaagccgtg gctcagcaaa ggcagctcaa gacggctgcc gagcttgccg
     2341 aactgatcga gtccgacctt gcgcgtcacc ctgactgtcc caaacgcgga tttcgagtga
     2401 cggtttacgg aggctcgcac tggcgcgcca tgttgacgat tacgccggct gccggccctg
     2461 ttcgcaaccc cggggagtgg cgagacctca ccaacgacct cgctgagcga ctccgcaaac
     2521 actacgattt ggcctgggaa tagcgcggtt ctgtctttgg ctcgaaccca agcggctttc
     2581 ccctcgggtt acgccaatcc actcggccca tggtggccga ccatgatccc acacttcgca
     2641 accgatgatg ccgaaagttc taattttacg aaacttttag ctattgccga tacggatatt
     2701 gatcggctcg gaacagcccc ccgaaacgct caggacgagc gatgacagat gcagaactat
     2761 cgatcatcga cgaaattaaa acgaccatac atattgggtc agcggaaaag cgcctggcaa
     2821 cggccaagca tgttaccgat cttttcctgt cgtccgccgg cagcttcaat catgagcaga
     2881 tcgagctatt tggcgatttg ctcgagcgcc tcataaaaac gatagagata agggcgatcg
     2941 cggacgtgag tgcccggatt gctctcgctg aaatgagcgt tcaacttgct ccggtcgccc
     3001 aggcaccgcc ttctgtcgtc cgccgtcttg ccatgaacga cgaaatcgcc gtcgccggac
     3061 ccgttttgac ggaatctgcc cgtctgagca cggaagacct ggtcgaaatc gcggaaacaa
     3121 aaggcgagca gcacctgttg gcgatttccg ggcgctggtg gttgaaggaa gttatcaccg
     3181 acgcgctatt ggcccgccgc tacccaaccg tcagccgtcg gatcgtcagc aatcccggtg
     3241 cacgggtttc cgccgccgga tttgccattg tcgtggcgca ggctgaatcc gaccctgaat
     3301 tggccattga aaccggaatc cgggtcgatt tgccgtcagc gcttcgtcgt caactgttgc
     3361 gcaatgcgac ggaagcggtc cgatcccggc tattgtcacg tgcgccaccg cacattttcg
     3421 aggaaatccg cagtgccatc gcgaccgtcg ccgcgggtgc cgatcgcgaa atgtccaacg
     3481 ttcgggactt taccgcggcc aagcgtttgg tggcgcggct caagaacaac gccgaactca
     3541 atgaggcgac cttgttcggc ttcgccaaac agcgaaaata tgaagagacc gtcgcagcgc
     3601 tcgccgagct gtcgcgatcc accatcgaga tcatccgtcc gctgatgcaa agccttcgcg
     3661 acgacggcgt tctctttccc tgcaaggccg cggaattgag ctgggagacc gtaagcgccg
     3721 tgctcgatag ccgctttgcg acgggctcca tggcgccgca tgaattggcc aaggcgaaag
     3781 cccaatttgc aaaaataacg cctgaaaacg cgcgccgtct gctcaggttt tggcaggtcc
     3841 gatcgtcacc ttcgccatca cgcgtaaact gacccgtcgt taacggcccg gcgcgcttca
     3901 cgggcgctcg tctcgtctta gcatcttgtt aagactatta cgcgaatcgc gggtcgtttt
     3961 aagccttctt gggcactcgg ttcttttaat gccgcattag ctgttgtcga aatcccgtaa
     4021 ctcgcctgtc aacccaagaa cgaaactggt cggatgactg aagcaaaatc gcttctggca
     4081 gaactggaag atgcggtctc cagaggaacc gccgaaagct gcctgcaggc cttgtggcac
     4141 gcgaccgata ttctgatcgc cggccgctat tccgaagaac agatctggac tttcggcgaa
     4201 atcatcgggc ggcttgccga agacatcgaa atcgccgcgc gcgtgcggct tgcaaacaag
     4261 cttgctccca gcagcaacgc gcctgtcaaa gtcatcaaac aacttgcttt tgacgattcg
     4321 atcgacgtcg ccggccccgt ccttcgccaa tccggacgga tcgacgccaa cacgctcgtt
     4381 gccaatgcac gatccaaaag ccagcagcac cttcttgcca tttcaaaacg aaaatcgatt
     4441 ccccctgagg taaccgacgt tttggtggtg cgtggcaccg cggaggttgc cacctcggtc
     4501 gcggcaaacg atggcgcgcg cctttcaggt tccggctttc tgcatcttgt aaagcgctcc
     4561 gaggatgacg gcatccttgc cgagcatctc ggtctgcgca aagacattcc caggcacgtc
     4621 tttcagcagc tgatttcgaa ggcatccgac gaggtcaaga aaaagctcga gcgagagcgc
     4681 cccgagatgg ggagccagat acaagccgcg gtcacggacg tcaccggcac gattcacgca
     4741 aaatttggac cggcgtcgaa aaactatttc gccgcaaaac ggatcgtggg caaactgcat
     4801 gaatatggca gcctgaatga agacaagatt tttgagttcg cgcattccct caaattcaac
     4861 gagaccgcgg tcgctttgtc cttgctatgc gtgttgccga tcgacgtcgt cgaacgcgcc
     4921 ttggttgaca agaacaggga agtcatcctg attctggcca gggccctgga tctttcatgg
     4981 accacgacca tgtcgttgct gtttctgggt gcggccaatt atcgaattac cgcaggtgac
     5041 ctcgaccgca tgaagcgtga atttgcccgg ttgaatgtcg aaacgtcaag acaagtactg
     5101 aaagtttatc agtcccgcaa ggaaacggcg gccgacagtt ccggcctccg ccccttgcct
     5161 cagctgcac
//
LOCUS       DASWGM010000500         8631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1558805,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000500 DASWGM010000000
VERSION     DASWGM010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558805"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VGA15_14295"
     CDS             complement(<1..738)
                     /locus_tag="VGA15_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9588901.1"
                     /translation="MREYVASVATRRLWRIAVLTAAGAIGAASHAEAALYYWSDSEPG
                     FSQFGPPVAQRRQQARRRQGKKVEASLKQAAKPQGPLIIAISIQNQRLKIYDAAGFFA
                     ETPISSGMAGHPTPMGVFSVIQKQKLHHSNIYSGAPMPYMQRITWSGIAIHAGVLPGY
                     PASHGCIRMPMAFAVKMWNWTKMGARVVVTPGEMTPANFSHPLLVAQKVVPQPSVADE
                     PKPDAPAATKTDKASDANGAVKSTISEA"
     gene            950..1495
                     /locus_tag="VGA15_14300"
     CDS             950..1495
                     /locus_tag="VGA15_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEX9588902.1"
                     /translation="MTSQAVSPYDKKWLPLVLFLLAGVLWLGSAFAADEPDLIFRRST
                     VFKFISPNDKLATYGVDDPEVEGVACHFTVPEKGGFKGWLGLAEEVSDISLACRQIGP
                     IHFKGKMEQGDDMFRQRRSLFFKKMQIVRGCDAKRNVLVYMVYSDKLIEGSPKNSTSS
                     VPIMPWGAADAAVQKCGEFVQ"
     gene            complement(1524..1817)
                     /locus_tag="VGA15_14305"
     CDS             complement(1524..1817)
                     /locus_tag="VGA15_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588903.1"
                     /translation="MGNGNIDVGKKTPTRKGGQRIIERLTGRDGSLALRIDDPNPRQK
                     EPAKVCGAMSQQYGDGGRGNESAPQKFVHLVGYRDPKPRPEPWVRPSVSPVKR"
     gene            2206..3042
                     /locus_tag="VGA15_14310"
     CDS             2206..3042
                     /locus_tag="VGA15_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9588904.1"
                     /translation="MTLRTAIGAMNQRMGDEESGSDKNGFERFRQSHQKVSVERSQPT
                     CLVVEDDRTMRHLVMNYLEEHGIRTISASGREEVAGLLAREEPSLVILDLRLGQEDGL
                     DLLREIRSHSDIPVIITTGHRRDEIDRVVGLELGADDYITKPFGLRELLARIRAVLRR
                     REAGQAAVQRDPERGRCRFGGWELDRRIRRLSDSSGIPVALTKGEYALLIAFLDAPQR
                     PLSREHLLQATRIHEDVFDRSIDVQVLRLRRKLETDPNAPRVILTERGVGYVFALPVE
                     PL"
     gene            3776..4816
                     /locus_tag="VGA15_14315"
     CDS             3776..4816
                     /locus_tag="VGA15_14315"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX9588905.1"
                     /translation="MIRSWSPTLRRRYPSEISVARWSVVPAILRARRRSLLWIFLALP
                     LAGGAAGQAPDHQASDARPLADQEPITPVPPPPAADPQKLALGERLFGDPRLSGDGKL
                     ACTSCHDLKTNGAGDGRTITAHDGSKLPFNALTVFNAALSFRLNWEGNFRALGAQAES
                     SLENPANMRTSVDEVLGKLNADPRMVQQFRAAYGRAPDRASFLDALVTFERSLLTPGS
                     RFDRWLGGDASALSTEELDGYQLFKSLGCSSCHQGVNIGGNLFERQGIFRPLVPAKPE
                     IVRVPSLRNVATTPPYFHDGSAPTLQEAVHRMAAAQLDRTLSDQQVDCIVAFLGTLTG
                     TYRGAAVLGAPQ"
     gene            4837..7326
                     /locus_tag="VGA15_14320"
     CDS             4837..7326
                     /locus_tag="VGA15_14320"
                     /inference="COORDINATES: protein motif:HMM:NF010411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system VirA-like sensor kinase"
                     /protein_id="HEX9588906.1"
                     /translation="MVPLLVVLLTWLSVCAINPDAELFDRALGEIDRFAMTENALYRD
                     VFTARAGILRNYDPLVREINELRAMLDRLREAAAVDSETTMAVDRLVASIGRQEALVE
                     QFKSDNALLHNSLSFFGRFSVRLASTGLGPAISAAAAAMLHLTLDTSSAAAGEVEDRL
                     DEVARQARSSSDADSVRALLAHGRLLHDLLPAVDNALKAMRAVPRKRDQDALRAMVLT
                     RQGASRTTARQFRRLLYATSLLLVAVLVLLGLRLRARAKALQRRAAFEHVIAGISMRF
                     INAQPQNIDTEIERALADMAGCIGCDRAYFVLSGPAPRTHRWCKDGMSFSPGWPENAP
                     ALAARLGPTVDGIIHVPRVKRMPPGENKDACIALGLGGWGCVTHVDNDGTRVALGFDA
                     IGRRCRITAAGELSLLRMALDTILSAVGRHAMEKERTRLETRLQQARRMETVGTFTSG
                     LAHNFNNILGGILGHSEVIEEHFGSDARLLRNLGAIRRGAERARDLVDQILTFGRRRD
                     ARRRPLSARALVTEAESLLNVSLPPGIDLAIHEPPAAAVVSGELAQLQQVILNLCNNA
                     AQAMENAGRIEVETEVHEVTAERSLSHGELQPGRYVRISVRDAGRGMEGATLARIFEP
                     FFTTRSAGNGLGLATVREIVREHGGAINVQSAPGEGSRFEVWLPGLATAGPASEADEP
                     ILPLGCGETVLMVANDGARLLRDEEMLAALGYEPVGFTGVDAALAACRATPERFDALV
                     VGEFGSAICSLELAAALHAAAPRLPIVLATKSIEEIGADTLVAAGIADVVRWPIAAQE
                     IATALNHCSALGKPDAGAPLSSARGARSLMH"
     gene            7605..8450
                     /locus_tag="VGA15_14325"
     CDS             7605..8450
                     /locus_tag="VGA15_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9588907.1"
                     /translation="MPILFCLVILFLIAAMLAWSGVRAWRSKNQFLKWGGAGSACLLA
                     LVVSVGIVLLGGGLLKLNARTAPVPALKVAGTPGQIQRGRAIADSFCGACHTKTGTLT
                     GGVDIGEHFPIRVGSFVSSNLTPAGQLRNWSDGEIFRAIRNGIDASGRWLIVMSYTNA
                     GKLSDEDTEALIAYIRSQPAAGETTPDPPDQLNPLGLMMLAAGMLPGGKPVFSGAIKA
                     PPHSPTAQYGEYILSFQDCRECHGGDLKGGVQGQLPPIGQSEIGATFTKIAKPTLQCM
                     FEPRN"
BASE COUNT         1552 a         2617 c         2765 g         1697 t
ORIGIN      
        1 cgcttccgaa atcgtggact tcaccgcccc attcgcgtcc gacgccttgt cggttttcgt
       61 ggccgcaggc gcatccggtt tcggctcgtc ggcgacactc ggctgcggca caaccttctg
      121 tgccaccagt agcggatgcg aaaaattggc gggcgtcatc tcgccgggtg tgaccacgac
      181 ccgcgcgccc atcttggtcc agttccacat cttcacggca aatgccatcg gcatgcggat
      241 gcaaccatgc gatgccggat aaccggggag cacccccgca tggatggcga tgcccgacca
      301 cgtgatccgc tgcatgtaag gcatcggcgc gccgctatag atgttggagt ggtgcagctt
      361 ctgcttttgg atgacgctga aaacgcccat cggcgttgga tgaccggcca tgccgctgga
      421 aatcggggtt tccgcgaaaa acccggccgc gtcgtaaatc ttcagccttt ggttttggat
      481 cgagatcgcg atgatcagcg gtccctgcgg cttggctgct tgcttcagcg acgcctcgac
      541 cttttttccc tgacgacgcc gagcctgctg ccgccgctgc gcgaccggcg gaccaaactg
      601 cgaaaatcct ggctcggaat ccgaccagta atacagcgcg gcttccgcat gcgaggcggc
      661 gccgatcgct ccggctgccg tcaaaaccgc aatccgccaa agccggcggg tcgcaaccga
      721 cgccacatac tcacgcacgc ataccatcct cgaatcccag tctcatgttt ggttgtcgca
      781 gacgagaaac gcgttcacca gcgcaaggcc tcgacccccg cgtttttcgc cccgagcgta
      841 gcaaaaaagc ctcaccatca gttaacgccg gacaatggcg gtcatcgggc agccttcctg
      901 ccggacttcc gcgcactaca taggtggaag cccgccgacg gagaacttca tgacatcgca
      961 agccgtaagc ccgtatgaca agaagtggtt gcctctggtt ttgttcctgc ttgcaggggt
     1021 gctgtggctg ggttcggcgt tcgccgccga cgagccggat ttgattttcc ggcgctcgac
     1081 cgtattcaag ttcatcagcc cgaacgacaa gcttgccacc tatggcgtcg acgaccccga
     1141 ggtcgagggc gtggcctgcc attttacggt gccggagaag ggcggcttca agggctggct
     1201 ggggttagcc gaggaagtgt ccgacatttc actggcctgc cgccagatcg gtccaatcca
     1261 cttcaagggc aagatggagc agggcgacga tatgtttcgg cagcggcgct cgctgttctt
     1321 caagaagatg caaatcgtcc gtggctgcga cgccaagcgg aacgtgttgg tctatatggt
     1381 ctattcggac aagctgatcg agggatcgcc gaagaattca acttcctcgg tgcccatcat
     1441 gccctgggga gccgccgatg ccgcggtgca aaaatgtggc gaattcgtcc agtgacggga
     1501 atctgaccga agggtccctg agtttatcgc tttaccgggc tcacgctcgg ccggacccat
     1561 ggttcaggtc gcggtttcgg atcgcggtag cccaccaggt gcacgaattt ttgcggggct
     1621 gattcgttgc cgcgaccgcc gtcgccgtac tgctgcgaca ttgcgccaca aaccttggcc
     1681 ggctctttct gccggggatt tggatcgtcg atccgcagcg cgagagaacc gtcacgccct
     1741 gtcaaacgtt caatgatccg ctggccgcct ttgcgggtcg gggttttctt gccaacatcg
     1801 atgttgccat tgcccacgac taaagtcctt ttgaagtaca tcgcaaccgc agctaactcg
     1861 gaaaagttgc agatcgttcg atcgagccgg ccggagcgac gcttcgggtc acgcttggcg
     1921 aattcccgag ccgcttttcc accagagaaa ggttaaaaat tcggccgatg cgtcgaaccc
     1981 ggtctccgtc gggcagcgca gccaatcctc tgtgcccgga aacggcatag acggccgcag
     2041 gcagcaatgt cgtccacttt ttgtcgcgaa atggccgcgt tcgttgaacg gctgacgctc
     2101 tgaacatcgc gtcaatgtta ccggtgatag agatcgtcgc tggtattatg ctgcagtgcg
     2161 gaatgtataa tgcatacact cgattagctc ttagagaaat tgcagatgac cctcaggacc
     2221 gcaattggtg cgatgaacca acgtatgggc gatgaagaaa gcgggtcgga caagaacgga
     2281 ttcgaaaggt ttcgtcagtc ccaccaaaaa gttagtgtcg aacgtagcca gccgacatgc
     2341 cttgtggtgg aggacgaccg caccatgcgg catctggtga tgaactacct tgaagagcat
     2401 ggcatccgga ccatctcggc ttccggacgg gaggaggtgg ccggcttgtt agctcgcgag
     2461 gagccgagcc tcgtgatcct cgacctgcgg ctcggccagg aggacggtct cgatctgctc
     2521 cgcgagatcc ggtcccattc ggatattccc gtgatcatca ccacgggtca tcgtcgcgac
     2581 gaaatcgatc gggtggttgg actggagctg ggtgcggatg actacatcac caagccattc
     2641 ggtctgaggg aattgctggc gcgcatccgg gcggtgctgc gccggcggga agccggacag
     2701 gccgccgtcc agcgcgaccc ggaaagaggc cgctgccgat tcggcggctg ggagcttgat
     2761 cggcgcatcc ggcgcctcag tgattccagc ggaatcccgg tcgcgctgac caagggcgaa
     2821 tatgcgctgc tgattgcgtt tctcgatgcg ccgcagcgac cgctgtcccg cgaacacctc
     2881 ttgcaagcaa cacgtatcca cgaagacgtg ttcgaccgca gtatcgacgt tcaggtgctg
     2941 cgcctgcgac gcaagcttga gaccgatcca aacgcgcctc gtgtcatcct gaccgagcgc
     3001 ggcgtcggct acgtgtttgc actgcccgtc gaaccgctct gacgatgccg cgccgaccag
     3061 aacggcatga gaaagcgagg cgaacgccgc gacacgcggg gctaccggtt ttcggagccg
     3121 gaaaggtggc gtgccgcgtt acaaccgtag cctcaatctc cggcgctgga atattgcagc
     3181 aatgtgcgca aggcagaatc tcccgcgtta gtagttgttg ggagaggtcg atgcaatttg
     3241 aaaaccggtt acctggcgac cgatcgtcgc gcaggcttat caatccgaga gaccagacgg
     3301 caatattttt gcgatattcg gcggcggaag ctggcacaag gctcggggga ccatccgcag
     3361 cctcggcgcc gcgggtttgg tcataccgcg aaatctaccg gcccaacgac gacggcagag
     3421 cgctccggac gtcgtcagat gacgctaggt cgcgccgcgc gatttccgca gcagcgtctg
     3481 ggatgatggc gagggtcgtc gggggccttg ccaagttggg tgagaccgca tttccgcgcc
     3541 ttctgttcgc ggtcatgtcc tggacgatgg cacaggctct ggcgggctgc attgcttatg
     3601 ccgaagcgat gtattccgtg gacctgagcg atcccgtcga ttgccgccat ccggcggcgg
     3661 gcctgcaacc ggagcgtgaa gatcgcgatc agattcggag caatcggatg ctgcgagacc
     3721 ttggaatttc tcgctgcgac atggaatcct ttgcgcggcg cggagacgtt tgcgaatgat
     3781 acgatcgtgg tcgccgacct tgcgtcggcg atacccttcg gaaatttccg tcgcgcgctg
     3841 gtctgtggtg ccggccatcc tgcgcgcgcg ccggcggtcg ctgctgtgga tctttttggc
     3901 gctgcccttg gcgggcggag cggctgggca agctccggat catcaagcct ccgacgcaag
     3961 gccgttggcc gatcaggaac cgataacacc cgttccgcca ccgcccgcgg ccgatccgca
     4021 gaagttggcg cttggcgagc gtctgttcgg cgacccgcga ttgtccggtg acgggaagct
     4081 tgcatgcacg tcctgtcacg acctgaagac caatggtgcc ggcgatggcc gaacaatcac
     4141 ggcgcatgac ggatcgaagc ttccattcaa cgccctgacg gtcttcaacg cggcgctcag
     4201 ctttcgcttg aactgggagg gcaactttcg ggcgctcggg gcgcaggccg agtcgtcgtt
     4261 ggaaaacccg gccaacatgc ggaccagcgt tgacgaagtt ctcggcaagt tgaacgccga
     4321 tccgcggatg gtgcagcagt ttcgcgcggc ctacggtcgg gcgccggatc gggcgagctt
     4381 cctggacgcc ctggttacct tcgaacgatc cctgctcacg ccgggaagcc ggttcgaccg
     4441 ctggctcggc ggtgatgcgt cggcgctctc gaccgaggaa ctggacggct atcaattgtt
     4501 caagtcgctc ggctgcagct cctgccatca gggggtgaat atcggaggca atctgttcga
     4561 acggcagggg atcttccgcc cgctggtccc ggcgaaaccc gagatcgtgc gagtgcccag
     4621 cctgcgcaac gtggcgacga caccgcccta ttttcatgac ggcagcgcac cgaccctcca
     4681 ggaggcggtg cacaggatgg ccgcggcgca gcttgatcgg acgctgtcgg atcaacaggt
     4741 cgattgcatc gtcgccttcc tcgggacgct gaccggaacc tatcgcggtg cggctgtcct
     4801 cggggcgccg caatgagggt tacaccggcc gcagcgatgg taccgcttct tgttgtgctg
     4861 ctgacctggc tgtcggtgtg cgcgatcaat ccggatgcgg aactgttcga ccgtgccctc
     4921 ggggagatcg accgtttcgc aatgacggaa aacgcgctgt atcgcgatgt cttcacggcg
     4981 cgcgccggca tcttgcgcaa ctacgatccg ctggttcggg aaatcaacga actgcgcgct
     5041 atgctcgatc gattgcgcga ggccgccgcc gtcgattccg agacgaccat ggcggtcgat
     5101 cgacttgtgg cgtcgatcgg caggcaggag gcgttggtcg aacagttcaa gagcgacaat
     5161 gccctgttgc acaactcgct ttcgttcttc gggcggttca gcgtccgcct cgcgtcaacg
     5221 ggcctcggtc cggccatcag cgccgcggcc gccgcaatgc tgcatctgac gctcgatacc
     5281 tcgtcagcgg ctgcgggcga ggtcgaggac cggttggatg aagtcgcccg gcaggcgcgc
     5341 tcctccagtg atgccgattc cgtcagggcc ctgctggcgc acggacggct gcttcatgac
     5401 cttcttcccg cggtcgacaa tgccttgaag gccatgcgcg ccgtgccgcg aaagcgagac
     5461 caggatgctc tgcgtgcaat ggtgctgacg cgccagggcg catcgcggac aacggcacgg
     5521 cagtttcgcc ggctgctcta cgcaacctct ctgcttctgg tcgccgtcct ggttcttctc
     5581 ggcctgcggc tgcgggcccg ggcgaaagcg cttcagcgcc gtgcggcctt cgagcacgtg
     5641 atcgcaggca tctcgatgcg cttcatcaac gcgcagcccc aaaatatcga caccgagatc
     5701 gaacgggccc tcgccgacat ggcggggtgc atcggctgcg accgcgctta ttttgtgctg
     5761 tctggtccag cgccgcggac gcaccgctgg tgcaaggatg gaatgagttt ttcgccgggc
     5821 tggccggaga acgcgcctgc gcttgcggca cgattgggcc cgaccgtcga tggaattatc
     5881 cacgttccac gcgtcaaacg gatgccgccg ggcgaaaaca aggatgcgtg cattgccctt
     5941 gggctggggg ggtgggggtg cgtcactcat gtcgataacg acggcactcg cgtcgcgctg
     6001 ggtttcgatg cgatcggccg gcgttgtcgc atcacggcgg ccggcgaact cagcctgttg
     6061 cgcatggcgc tcgataccat tctgtctgcg gttgggcggc acgcgatgga aaaggaacgg
     6121 acacggctgg agacgcgcct gcagcaggcc cgccgcatgg agacggtagg cacattcacc
     6181 agtggtctgg cccataactt caacaatatt ttgggcggga ttctcggcca ttcggaagtg
     6241 atagaagagc acttcggttc ggacgcccgg ctgcttcgca atctcggtgc gattcgccga
     6301 ggcgcggagc gggcgcgcga tctcgtcgac cagattctga cgttcggacg ccgccgcgat
     6361 gcgcgtcgtc ggcccctcag cgcgcgggct ttggttaccg aggcggagtc gctgctgaat
     6421 gtctcgctgc cgccggggat cgatcttgcg atccatgaac cgccggcggc ggccgtcgtg
     6481 tcgggcgagc ttgcccaact gcagcaggtg atcctcaatc tctgcaacaa cgcagcgcag
     6541 gcgatggaaa acgccggccg catcgaggtc gagaccgagg tgcacgaagt taccgccgag
     6601 cgttccctca gtcatggtga gcttcaaccg ggccgttacg tgcggatctc ggtgcgcgat
     6661 gccggccgcg gcatggaggg ggctacgctg gctcggatct tcgagccttt tttcacgaca
     6721 cggtcggccg gcaacggcct gggattagcg acggtacgcg agatcgttcg cgagcacggc
     6781 ggagcgatca acgtgcagag cgcaccgggc gagggcagcc gtttcgaggt ttggctgccc
     6841 ggcctggcga ccgccgggcc ggcgtcggag gcggacgagc cgatactccc gctgggatgt
     6901 ggcgaaacgg tactgatggt cgccaacgat ggcgcgcgat tgctgaggga cgaagagatg
     6961 ttggcggcat tgggctacga gccggtcgga ttcaccggcg tcgacgcggc gttggccgcg
     7021 tgccgcgcga cacccgagcg gttcgacgcg ctggtcgtgg gagagttcgg atccgccatc
     7081 tgctcgctcg aactcgcggc agcgctgcat gcggcggcgc cccgtctgcc gatcgtgctg
     7141 gcgaccaaat cgatcgaaga aatcggcgcc gatactttgg tggccgcagg tatcgcggac
     7201 gtcgtgcgtt ggccgattgc agcccaggag atcgcgacgg cactcaatca ttgttcggcg
     7261 ctcgggaaac ctgacgcggg ggcgccgctc agttcggcac gcggcgcccg ttctctgatg
     7321 cattgagccg atcggcggcg cgacggctat tggttcacca acgcgcaggc gtgcgcagcc
     7381 ttttccgctt ccgggtggat taccgccgta accgttctct ccgcgtggaa atcgtgccgt
     7441 aacacggctc aaccaatctg ttcgatgctg ggtcggcgtc gcgttcttcg gggcggagtg
     7501 caacgtgatt ttcggccaga cattcatcgt gaccagcgcg cagctcggtc gaaccggcca
     7561 atcaacgctt ccaaatccca agcagtcaac gccaaggaga tataatgccc atcctattct
     7621 gcctcgtgat attgtttctg attgccgcaa tgctcgcttg gtccggcgtt cgcgcctggc
     7681 gcagcaagaa tcaatttctg aaatggggcg gtgcgggttc ggcttgtctg ctggctttag
     7741 tcgtttcggt cggcatcgtt cttctgggcg ggggtctgct aaaactcaac gcgcgcaccg
     7801 cccccgtccc agccctcaag gtcgccggga ccccgggcca gattcagcgc ggccgtgcca
     7861 tcgccgacag tttctgcggt gcctgccata cgaagaccgg gacactcacg ggcggcgtgg
     7921 acatcggcga gcattttccg attcgcgtcg gctcctttgt atcttcgaac ctgacgccgg
     7981 ccgggcaact gagaaactgg tcggatggcg agatttttcg agcgatccga aacggcatag
     8041 atgccagcgg tcgttggctg atcgtgatgt cgtacacgaa tgcggggaag ctgagcgacg
     8101 aggatactga ggctctgatc gcctatatcc gcagtcagcc tgccgcagga gagacgacgc
     8161 cggatccgcc tgatcagctc aatccgctcg ggctgatgat gctggctgcc ggtatgctac
     8221 ccggcggaaa acctgtattt agcggcgcca tcaaggctcc tccacacagt ccgaccgcgc
     8281 aatacggcga atacattctg tcgtttcagg attgccgcga atgccacggt ggcgatttga
     8341 aaggtggcgt gcagggacaa ttgcctccga tcggtcaatc cgagatcggc gcaaccttta
     8401 caaagatcgc aaagccgacg ctgcaatgca tgttcgagcc gcggaactga agcgcgacgc
     8461 ggtctggcgt gaattggtcg gcagatatgg cagctggtag gtgatgtcgg cgctacgctc
     8521 gccgagcagc gatccggccg catctatttc cgcttgatcc ccaaacgcgg aatacatttt
     8581 gtcgcagcga ggattcccgt cggggtcatc ttggcgccgg aaacttcctt g
//
LOCUS       DASWGM010000501         3863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1994808,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000501 DASWGM010000000
VERSION     DASWGM010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994808"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1144)
                     /locus_tag="VGA15_14330"
     CDS             complement(215..1144)
                     /locus_tag="VGA15_14330"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9588908.1"
                     /translation="MPDVRVLATDLEFPEGPVVLPDGSVVLVEIRGQRLTRVWPDGRK
                     QVVAEIPGGPNGAAMGPDGKIYICNNGGFSWIPSRGTMMPGAPEPHEYIGGSIQRVDL
                     ATGEVETLFDKCGKHSLKGPNDLVFDRHGGLWFTDLGKRRARDMDVGAFYYMKPGATE
                     VVEAVFGMMPANGIGLSPDENTVYVAETPTARLWAFDLSAPGEIRPRDVIYRGERGKP
                     IAGLGGYQMFDSLAVEASGNVCVATLISGCISVIAPDGTIVEQVPTGDRVTTNIAFGG
                     PDLKTAYITLSGKGELVAMDWARPGLALNFLNK"
     gene            complement(1382..2215)
                     /locus_tag="VGA15_14335"
     CDS             complement(1382..2215)
                     /locus_tag="VGA15_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9588909.1"
                     /translation="MNPAQKALWFIESHLARELSLDEIAGIAGISRFHMVRAFAAATG
                     LSVMRYVRARRLTEAARALANGAPDILTLALDADYGSHEAFTRAFRGHFGVTPEAVRA
                     STCLDGIKLQEPIQMDSTLTDNLHAPRFETSRPLLVAGISERYNHENGAGIPNQWQRF
                     HQAVENIPGRIGKVAYGVCCNGDDAGNFDYIAGVEVSDFSDLPREFYGVRIPEQRYAV
                     FTHRDHISTIRRTVNTIWNHWLPASGLRAADAPNFERYDTNFDPLTGNGGLEIWVPVR
                     D"
     gene            2477..3706
                     /locus_tag="VGA15_14340"
     CDS             2477..3706
                     /locus_tag="VGA15_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588910.1"
                     /translation="MHYRVLNHAKSVGLAGLLAVAVASAANAQKKYDPGATDTEIKIG
                     NIMPYSGPASAYATIGKTEAAYFNKINADGGINGRKINFISYDDGYSPPKAVEQARKL
                     VEADEVLLIFNPLGTPSNSAIQKYMNAKKVPQIFVSSGAAKWNDPKNFPWTIGWQPSY
                     QVEARIYAAYILKNYPGKTIGVLYQNDDFGKDYVIGLREGLGDQANKLIVVESSYETT
                     SPTVDSQVVQIKGANPDIFVNVSTPKFAAQAIKKIGELAWHPVQFLTNVSASVGGVMK
                     PAGYENDQDILSAAYLKDPQDPKWKNDPAMNEWRAFMTKWYPEGDQQDAATTFGYGVA
                     KGLEQVLRQCGDDLTRENIMKQAANLNFEIGVYLPGTHIKTSPTDFAPLEQLQMMRFK
                     GESWDLFGPIMSGEKNS"
BASE COUNT          806 a         1186 c         1157 g          714 t
ORIGIN      
        1 cgccccctcg aactcaaggc cggccggctc gttgctcgtc ttaaaaagct cggctttaac
       61 gtccaactcc aaccagcagc ggaggccgcc tgactcctct atccaacagc ttcttcctag
      121 gagcgcaagc gacgcagagc aatccagttt tacttcgcgg ctatggattg cttcgcttgc
      181 gctcgcaatg acgaaagttg gcatcggtta tcggttactt attcagaaaa ttcagcgcca
      241 gccctggccg cgcccagtcc atcgcgacga gctcgccctt gccggacagc gtgatgtagg
      301 cggtcttcag atccggcccg ccgaacgcga tgttggtggt aacgcggtcg ccggtcggca
      361 cctgctcgac gatggttccg tccggcgcga tcaccgaaat gcagcccgag atcaaggtcg
      421 ccacgcagac attgccggaa gcttccaccg ccagcgagtc gaacatctgg tagccgccga
      481 gccccgcgat cggcttgccg cgttcgccgc gatagatcac gtcgcgcggc ctgatctcgc
      541 cgggcgcgga cagatcgaac gcccatagtc gcgcggtcgg cgtctccgcg acatagaccg
      601 tgttctcatc cggcgacagc ccgatgccgt tggcgggcat catgccgaac acggcttcga
      661 cgacctccgt ggccccgggc ttcatgtaat agaacgcgcc gacatccatg tcgcgggcgc
      721 ggcgcttgcc gagatcggtg aaccagagac cgccatgcct gtcgaacacc agatcgttcg
      781 gtcccttcag ggaatgcttg ccgcatttgt cgaacagggt ttcgacctcg ccggtcgcaa
      841 gatcgacgcg ctggatcgag ccgccgatat attcatgagg ttcgggcgcg cccggcatca
      901 tggtaccccg cgacgggatc cagctgaagc cgccattgtt gcagatgtag atcttgccgt
      961 cggggcccat cgccgcgcca ttggggccgc ccggaatttc cgcgaccacc tgcttgcggc
     1021 catcgggcca cacccgtgtc agccgttgcc ctcgaatttc gaccagcacc accgatccgt
     1081 ccggcagcac caccggcccc tcggggaact caagatcggt ggcgagaacg cggacatcgg
     1141 gcatcggaat cctccggctt ttcttaaaag cgccggcgga ttcccaagct tattccgcat
     1201 gcgcaacgat ttcgttacgc gatgttatgg caaagccacg cgcgcttgcc aagcaatcgg
     1261 cggccagggg ctgcgcaagg cagccccgcg tgcggcccag cgtcgttatt cgacacgttt
     1321 tcttcgcgaa ccggtatcca tcccgaatcg cgtccgggac atgcttcgct cgaaaacgcg
     1381 cctagtccct gaccggcacc caaatctcga gaccgccatt gccggtgagc ggatcgaagt
     1441 ttgtgtcata gcgctcgaag ttgggcgcat cggccgctct cagacccgac gccggcagcc
     1501 agtgattcca gatcgtgttg acggtgcggc gaatggtcga gatatggtca cggtgcgtga
     1561 agaccgcgta tctctgttcg ggaatccgaa cgccgtagaa ttcgcgtggc aggtcggaga
     1621 agtcggatac ttcgacgccg gcgatataat caaagtttcc ggcgtcatcg ccgttgcagc
     1681 agacgccgta cgccaccttc ccgatccggc cgggaatgtt ctcgacggct tggtgaaagc
     1741 gctgccattg attgggaatg ccggcgccgt tctcatggtt atagcgctcg ctgataccgg
     1801 cgacgagcag cggcctgctg gtttcgaaac gcggcgcgtg aagattgtct gtgagggttg
     1861 aatccatttg aatcggctcc tgaagtttga tgccatcgag gcacgttgat gcacggaccg
     1921 cttcaggcgt gaccccgaaa tggccgcgga acgcgcgggt aaatgcttcg tgagagccgt
     1981 aatccgcgtc cagcgccagg gtgaggatat cgggtgcgcc atttgccagc gcgcgcgccg
     2041 cctcagtgag gcgccgggca cgcacgtagc gcatgaccga caaccccgtc gcggcggcga
     2101 acgcgcggac catgtggaag cgcgagattc cagcgatgcc tgcgatctcg tcgaggctca
     2161 gttccctcgc aaggtggctt tcgataaacc agagcgcctt ctgggccgga ttcatggacg
     2221 acagccttcg ttcgaagctc gaacatcatg atcgatttgc acgcgcttca tttgacagtc
     2281 cttgctctca ttcgtcgtta ggaaactgga accggacgcg atggtttctg ccgctccgcg
     2341 gaaaatacct gcggaggcgt cgctggcaac gtcaatccaa tcgtgcatac tgcgcccgca
     2401 ttcgatccga cggctcataa agctaagcta cacaaaaacg gcctgagcct cggccagcac
     2461 gtcagggagg acgatgatgc actaccgtgt cttgaaccat gccaaatccg tcggtcttgc
     2521 tggactgctt gcggtcgcgg tcgcgagcgc cgccaacgcg cagaagaaat acgatccggg
     2581 tgctaccgat accgagatca agatcggcaa catcatgccc tattcaggac cggcgtcggc
     2641 ctatgccacg atcggcaaga ccgaggcggc ctatttcaac aagatcaacg ccgacggcgg
     2701 catcaacggc cgcaagatca atttcatctc ctatgacgat ggctacagcc cgccgaaggc
     2761 ggtggagcag gcgcgcaaat tggtcgaggc cgacgaagtg ctgctgattt tcaatccgtt
     2821 gggcacgccc agcaacagcg cgatccagaa atacatgaac gcgaaaaagg tgccgcagat
     2881 cttcgtatcg agcggcgccg ccaaatggaa cgatccgaag aattttccgt ggaccatcgg
     2941 ctggcagccg agctaccagg tcgaggcacg catctatgcg gcctacatcc tgaaaaatta
     3001 ccccggcaag accatcggcg ttctctatca gaacgacgat ttcgggaaag actatgtgat
     3061 cgggctgcgc gaaggcctgg gcgatcaggc caacaagctg atcgtggtcg aaagttcgta
     3121 tgagaccacc tcgccgacgg tggattcgca ggtcgtgcag atcaagggtg ctaatcctga
     3181 catcttcgtc aacgtctcga cgccgaaatt tgccgcgcag gcgatcaaga aaatcgggga
     3241 attagcctgg cacccggtac agtttctcac caacgtgtcg gcttccgtcg gcggcgtgat
     3301 gaagccggcc ggttacgaaa acgatcagga catcctcagc gcggcctatc tgaaggaccc
     3361 tcaggatccg aaatggaaaa acgatccggc catgaacgaa tggcgggcct tcatgaccaa
     3421 gtggtatccg gaaggcgacc agcaagacgc agcgaccacg ttcggttacg gcgtggccaa
     3481 ggggctggaa caggtgctgc ggcagtgcgg tgacgatctc acccgcgaaa acatcatgaa
     3541 gcaggcagcc aatctgaatt tcgaaatcgg cgtgtatctg ccgggcacgc atatcaagac
     3601 cagcccgacc gacttcgcgc cgctcgaaca gctgcagatg atgcgcttca agggcgagag
     3661 ctgggatctg ttcgggccga tcatgtcagg cgaaaagaac agctgagcaa gcgtaatccc
     3721 tcccccttgt ggggagggtt ggcgcgtagc gccggggtgg gggtagcgca cggagtgcgc
     3781 aaaaaatctt tgcggaagcg gccccccacc cgaccggcct tcggccggcc accctcccca
     3841 cgagggggag ggataagaaa gca
//
LOCUS       DASWGM010000502         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2275778,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000502 DASWGM010000000
VERSION     DASWGM010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275778"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1151
                     /locus_tag="VGA15_14345"
     CDS             498..1151
                     /locus_tag="VGA15_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9588911.1"
                     /translation="MTPTITAFEGSPDGGKGLARDTRVRWALEEVGQPYEVRLVSFRA
                     MKEPAHLALHPFGQIPTYEEGDLGLFETGAIVFHIAERHAGLLPDDANARARAITWMF
                     AALSTVEPPILELGTARLLEGDKSWSAQRMPLVEDRVRNRLGQLSRRLGDADWLDGAF
                     SAGDLMMVSVLLRLKASGMLDDYPNLSAYVARGEARPTYKRAFDAQLAVNTGKQPTG"
     gene            complement(1593..>2540)
                     /locus_tag="VGA15_14350"
     CDS             complement(1593..>2540)
                     /locus_tag="VGA15_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9588912.1"
                     /translation="AAREAELLPIPYFHVVFTLPSAVGDIAYQNKAVIYDLLFKASSE
                     TMLGIAADPKHLGARIGITSVLHTWGSAMAHHPHLHMIVPGGGLSMDGGRWISCQPNF
                     LLPVRVLSKLFRRLMLEKLLAAHGAGRLQFLGSHAHLADAKTFAAFLAPLWKKRWFVY
                     AKRPFAGPGAVLAYLSRYTHRVAISNHRLIAVDQRSVTFKVKDYRIEGPGRYTTMTLD
                     VGEFIRRFLIHVLPKGFHRIRHYGLFASAKRAETIARVRELLGLGTAAAEEAVEIDPA
                     AQPLAQPCPCCGGRMFVIETFDAGCQPRYRPTAMRIDTS"
BASE COUNT          463 a          741 c          859 g          477 t
ORIGIN      
        1 ggcgactcgt cgcacatcag aacgccaagg cccgcatcgt cgcgctcaag cctgatgacg
       61 tcgtcgtcgt gacagggcat ttgccgggta accaggcgct cgccgcagtg ttaagggcca
      121 ttttcggact ggggcgcgcg caatggcgag gtccgcttta ccccggttga gaccaccctc
      181 aatgaactga cataatcagc tcgagcagcg acgtccgaga agtgccagaa gctgacataa
      241 gcggcgtcct ggacggcggt gcgggtctgc tcgcgatgac tgaagggttt taggtcgttc
      301 gccgataacg gccgcgcgca gaaggttcgg atcgcgaaat cccatggcca aggaaaacga
      361 ggtaagttgt cgggagccgc gcccgtcgtt cgtcctctgg gtgcaaagcg caagcaccga
      421 atacctaacc atgagaagat cgtcgtaatc acgcacacca ctctcgacag cgtcatgcaa
      481 ttacccggag ccttccaatg acccccacca tcacagcctt tgaagggtcg cccgatggcg
      541 gcaagggact ggcgcgtgat acgcgcgttc gctgggcgct ggaggaagtg ggccaacctt
      601 acgaggttcg tcttgtttcg ttcagggcga tgaaggaacc cgcacatctg gcgcttcatc
      661 ctttcgggca gattccgacc tacgaagaag gcgatctcgg cttgttcgag acgggggcga
      721 tcgtgttcca tatcgccgaa cgccatgcgg gcctgctgcc ggacgatgcg aatgcccggg
      781 cgcgcgcgat cacatggatg ttcgccgcgc tcagcacggt ggagccgccg atccttgaac
      841 tcggaaccgc caggctcctt gagggcgaca agtcctggtc cgcgcaacgc atgcctctcg
      901 tcgaggatcg cgtccgtaac cggctgggcc aactttcccg tcgccttggc gatgccgact
      961 ggctcgatgg cgcgttcagc gcgggcgacc tgatgatggt gtcggtgctg ctcaggttga
     1021 aagcgtcggg tatgctggac gactatccga acctctccgc ctatgtggcc cgcggcgaag
     1081 cgcggcccac ctacaagcgt gcctttgacg ctcaattggc ggttaacacc ggcaagcaac
     1141 cgaccggctg atgagggccg ccccggggtc ccttgacccc tcggagcaaa cgccggtttt
     1201 aggcggtgat gaacgttcgt tgagaatgtc cgcaactgtg aagggtttcg gatgacggcc
     1261 gatcgggacg attcggcttg caccgtggcc gtcgtccgaa agcctccaat gaaggaacgc
     1321 gcgtgaggtt ggtaggacgg ctgtcgcggg ctatggagat ttgatcgcgc ggatgtgggt
     1381 gtgcggaagt cggctgggct cggggatgac gtttttgcgc ctgctggttt ggctgatcgg
     1441 ctttgatgga gccgcgttga cgatgcgttt cggggatgca cgcggtgcgg aatgcaatgc
     1501 cggacccgtg ttcggccgag cggcggcgta tctggtcatt gaccagcgag gaagaccctt
     1561 ggtgattggg gtgctgaatg ttgcggtttt gctcatgagg tatcgatcct cattgccgtc
     1621 ggccggtagc gtggttggca gccggcatcg aaggtctcga tgacgaacat gcggccgcca
     1681 cagcaggggc aaggctgggc gagtggctgc gccgccggat cgatttcgac cgcctcttcg
     1741 gccgcagccg ttcccagtcc caggagttcg cgcactcgcg cgatcgtctc ggctcgcttc
     1801 gcgctggcga acaagccgta gtggcggatg cgatggaagc ctttgggcag cacgtggatg
     1861 aggaaccggc ggatgaactc gccgacgtcg agcgtcatcg tcgtgtaccg gccggggcct
     1921 tcgatcctgt agtccttgac cttgaacgtg acgctccgct ggtcgactgc gatcaggcga
     1981 tggttcgaga tggcgacgcg gtgcgtatac cgcgacagat aggcgagcac ggctcccgga
     2041 ccggcgaacg gccgcttggc gtagacgaac cagcgcttct tccagagtgg agcgaggaac
     2101 gccgcgaatg tcttcgcatc ggccagatgc gcgtgtgagc cgaggaattg cagcctgccc
     2161 gctccgtgtg ccgccagaag cttctccagc atcaaacggc gaaacagctt cgatagaacg
     2221 cgcacgggca ggaggaagtt tggctggcac gagatccaac ggccgccatc catggagagc
     2281 ccgccgcccg gcacgatcat gtgcaggtgc ggatggtggg ccattgccga gccccaggtg
     2341 tgcaacaccg acgtgatgcc gatgcgggct ccgaggtgct tggggtcggc cgcgatcccg
     2401 agcatcgtct ccgacgaggc cttgaacagc agatcgtaga tcacggcctt gttctggtag
     2461 gcgatgtcgc caaccgccga cggcagcgtg aagacgacgt ggaagtaggg aatcgggagc
     2521 agttcggcct cgcgcgccgc
//
LOCUS       DASWGM010000503        11697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_173145,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000503 DASWGM010000000
VERSION     DASWGM010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173145"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2229
                     /gene="dnaE"
                     /locus_tag="VGA15_14355"
     CDS             <1..2229
                     /gene="dnaE"
                     /locus_tag="VGA15_14355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX9588913.1"
                     /translation="YVQQRYGRDQVAQIITFGTLQARGVLRDVGRVLQMPYGQVDKLT
                     KLVPQNPAAPVTLAAAIASEPKLQAFRDEDAVVARAFDIAQRLEGLTRHASTHAAGIV
                     IADRPLSELVPLYRDPKSDMPVTQFNMKWVEPAGLVKFDFLGLKTLTVLDVAVKLLRK
                     RGVDVDLATLPLDDAASYQMLARGDVVGVFQVESQGMRRALVDMRPDRFEDIIALVAL
                     YRPGPMANIPTYCARKHGDEEPEYLHPMLEPILKETFGVIIYQEQVMQIAQVMAGYSL
                     GEADLLRRAMGKKIRSEMEKQRAGFVAGAMKNNVPKGQADTIFELLAKFADYGFNKSH
                     AAAYALVSYHTAYMKAHYPVEFLAASMTLDLSNTDKLSEFRAEAQRLGIKVEAPSVNR
                     SGATFEVSEGTIYYALAGLKGVGGQAVDLIVDARKDGLFTSLADFAARVNPRAINKRV
                     IESLAAAGAFDALDSNRARVFAGAEAILAACQRSHEAATVGQNDMFGGAADAPTIMLP
                     QVEPWLPAERLRREYDAIGFFLSGHPLDDYATVLKRLRVQTWAEFSRAVKTGATAGKV
                     AATVVSRMERRTKTGNKMGIMGLSDPTGHFEAVLFSEGLAQYREVLEPGAAVLLQLGA
                     ELQGEDVRARVLHAEPLDAAAAKTQKGLRIFVRDTKPLESIAKRLQMPEPAPQGSPVR
                     GPQTKPLPDAAGGANGDVSLVMMLDLETEVEMKLPGRFRVSPQIAGAIKAVSGVVDVQ
                     QL"
     gene            complement(2399..2821)
                     /locus_tag="VGA15_14360"
     CDS             complement(2399..2821)
                     /locus_tag="VGA15_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEX9588914.1"
                     /translation="MARTVVPRTPEQILGPFYPLKPLDQNADLTRVSGRPGRAEGQVL
                     TVMGRVLNLNGEPVRNAKLEIWQANAHGRYTHPSDTNPAPLDPNFEGAAILTADDDGR
                     YQFKTIKPAAYPVGPHLMRPAHIHFQVSGRQDRLVTQM"
     gene            3184..5508
                     /locus_tag="VGA15_14365"
     CDS             3184..5508
                     /locus_tag="VGA15_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9588915.1"
                     /translation="MRVTRSFSILSFCLILFCGFPGILSSAAAQQQEKRIALVVGDGA
                     YAKSPLATAANDAGLIAQTLQAAGFDVVGARDLDGDTLRRSFRDFIQKAEASGPDTVA
                     VVYLAGYGMQLAGENYFIPVDSTVSRDTDIPTEGMRINDYVRQLASLPLKAGVVVLDA
                     ARQQPFVEGGQPLASGLALVEPDAHMLIAFNAAPGTIAPTEQGPYGVYAQSLAEMIRT
                     GGLSLPEVFNRVRLRVNELSKGAQVPWDAEKIDGQFMFFNRAPDAPAPAAPDQAAAVR
                     GKPIRDLGAQDAYAAALERDTMQGYEDFLTAYPNDPLAKRVRAIAAARREAITWLRSY
                     RADTPQAYWSYLRRYPRGPHAGDARRRLAILIASLEPPPSFAVIDYDVPPPPPDEIVY
                     VDRPVLMFSDPEFDFAPPPPPPLFFLPPPPPDFIVLEPPPPPIGLFILPQPFFVPIPV
                     YVQQPVYVVPPPNNIIFANIHNTTVINNVINRPPQPGAGNVQPNAKGVAAPPGVKGAP
                     GQGGVAQPGTGPALPPAVAQRASLIQQGKLAVPPSASINPAARLGTPGTPSGQRVAPN
                     TPVPTNVSPNALPQALPKTNALPVPGAKGTPPAPPSAAAPVPGPPNPNAKLAPQDQPR
                     ASEPALRAGQPKAGGSAQAPEAKPVVPETVRRPNAAPPPPPPPKPAAAPPKPAPRAEP
                     KPQMQRAEPPHRAAPPPRPAPQMARPSPPPPRAAPPPPRMAAPPPPRPAPPPPRPAPQ
                     IARPAPPPPRPAPPPPRPAAPAAAKKCPPNVPRC"
     gene            5771..6280
                     /locus_tag="VGA15_14370"
     CDS             5771..6280
                     /locus_tag="VGA15_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HEX9588916.1"
                     /translation="MLSLAAASLVGCASGRIGADYATVTQKVGPPKAGQARIVLLQEK
                     ASGLGYGSCASDMKLDGDPMGRLKPGTYLYADRPAGHHQLLAAETMFPGDSKRDITME
                     PGRTYFFLAKVSERHNTLTGMTVAGGLAGMLVSSAVTSGSENPGPVDFFLLDEPGARA
                     TLAELQLAE"
     gene            6444..7709
                     /locus_tag="VGA15_14375"
     CDS             6444..7709
                     /locus_tag="VGA15_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX9588917.1"
                     /translation="MDGGAARAAPDASSAAQAIYAAAPPRLLQIRTLVADAGRQTSIG
                     SGFLVSPDGLAITNYHVVSQVALEPKTYRLEYAAADGSHGDVSLLGVDLPNDLAIIRL
                     DKHDVPYFAFDEAAMAGGLPQGERLYSMGNPLDLGFTISEGTYNGLVGHSYNDRIHFT
                     GALNPGMSGGPTVTADGLIVGINVATRRGGQLVSFLVPARFAAALLQRVREHEMPSDL
                     HAEIARQLAAWQSGLYKSFGGEGFRSTTVGPYRAPETMAPWFDCWAQTNAGATPKPRA
                     SINSTNCASDTGLFVAADLNTGAIQLSHSYVRTIDLNQFQLATVLTQLSQPRLVGGGP
                     FRKWYTPERCHEDFVSAEPASDHPPLRVIWCAQAYREFEGLYDVALIAVTQDHGSEAL
                     VSRLSLQAVGYDDAMALGKRFLEAVQVSK"
     gene            7706..8668
                     /locus_tag="VGA15_14380"
     CDS             7706..8668
                     /locus_tag="VGA15_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX9588918.1"
                     /translation="MIWIEILSRHRDITARFRIAGPEAYIGRGYDNDVIVDDPYVAAQ
                     HLRIFRDETGQLVAEDMGSANGLFLDGGKSRQARIVVDGSHPIRIGQTFLRIRETSHA
                     VERERVARPERRIFPIVLAAALGVVILGFDTVKVWLTQTSEPRVSGYLTPLLVMAATV
                     LAWVGVWALLTRIFSGHSRFLRNLIIAQVGILAFSLYDQGARFSAFAWTWSTASSYQY
                     VAAWSIVAVVCFLHLREVGPARLLFKGALVTTLLATAIAVQTLQRSEAFSDSGRQTTA
                     SLLMPPALRLAPLRDEGAFFGEIAKLKTTLDGDRTEARADQAGR"
     gene            8811..9146
                     /locus_tag="VGA15_14385"
     CDS             8811..9146
                     /locus_tag="VGA15_14385"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9588919.1"
                     /translation="MTAGFHANATLFTACGGPSLMPRRLLRGSDVYHSMQLYTSQGSP
                     MRTNIDIDDTLMAEAQEASGHATKKQTVEQALRLMIRLRRQQEASAAFGKYRWRGNLA
                     RSRKGRGIS"
     gene            9143..9544
                     /locus_tag="VGA15_14390"
     CDS             9143..9544
                     /locus_tag="VGA15_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HEX9588920.1"
                     /translation="MIVVDSSVWIDFLNGRNAPHVRRLRAVLGTDEVIVGDLMLCEVL
                     QGLDSERAARDVEALLRRFDIVPMAGDPIAVAAARNFRSLRRRGITVRKTIDLLIGTW
                     CIENRRPLLHNDSDFRPMARHLGLIEAPMTA"
     gene            9763..10761
                     /locus_tag="VGA15_14395"
     CDS             9763..10761
                     /locus_tag="VGA15_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX9588921.1"
                     /translation="MALPDFSMRQLLEAGVHFGHQSHRWNPKMADYIFGARNNIHIID
                     LAQTVPLLHRALQAVSDTVAKGGRVLFVGTKRQAQDGVAEAAKRSAQYFVNSRWLGGT
                     LTNWKTISGSIKRLRHLDEVLNSGDANAYTKKERLTLQRERDKLDRSLGGIKDMGGLP
                     DMIFVIDTNKEDIAIQEAQRLNIPVAAIVDTNSDPKGITYVVPGNDDAGRAITLYCDL
                     VARAVIDGISRAQGESGVDIGASAHPVREEITVAPQPAAFQGLAGPRGAADNLKKLTG
                     VSGAIEKKFNDLGIFHYWQLAELDHDTAHKIGEEVGLPSRADGWVAQAKAMTAEAE"
     gene            10886..>11697
                     /gene="tsf"
                     /locus_tag="VGA15_14400"
     CDS             10886..>11697
                     /gene="tsf"
                     /locus_tag="VGA15_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX9588922.1"
                     /translation="MATITAAMVKDLRETTGVGMMDCKQALAENDGDMQAAIDWLRKK
                     GLSKAAKKAGRVAAEGLIGALTAGTKGVVVEVNSETDFVARNEQFQGLVKMIAQVALD
                     VGADVEKIKAAKVGSVTVETAIADAIATIGENMTLRRAASLEVGKGVVSSYVHNAVIE
                     GAGKMGVIVALESTGKTDELAALGRQLAMHVAAANPQALDPAGLDPAVVKREKDVLAD
                     KYRQQGKAENVIEKIVESGLKTYYKEVCLLEQAFIHDSAKSVGQAVKEAEGK"
BASE COUNT         2159 a         3735 c         3715 g         2088 t
ORIGIN      
        1 tatgtgcagc agcgctatgg ccgcgatcag gtggcgcaga tcatcacctt cggcaccttg
       61 caggcgcgcg gcgtgctgcg cgacgtcgga cgcgtgctgc agatgcccta cgggcaggtc
      121 gacaagctga ctaaactggt gccgcaaaat ccggcggcgc cggtgacgct ggccgccgcg
      181 atcgcgagcg aaccgaaatt gcaggcgttt cgcgacgagg acgccgtggt ggcgcgtgct
      241 ttcgacatcg cccagcgcct cgagggcctg acccggcacg cgtccaccca tgctgccggc
      301 attgtaatcg ccgatcgtcc gctcagcgaa ctggtgccgc tctaccgcga tccgaaatcc
      361 gacatgccgg tgacccagtt caacatgaaa tgggtcgagc ccgcgggcct cgtcaaattc
      421 gacttcctcg gcctcaagac gctgacagtg ctcgatgtcg ccgtgaaact gctgaggaag
      481 cgcggggtcg acgtcgatct cgcgaccctg ccactcgacg atgccgccag ttaccagatg
      541 ctggcgcgcg gcgatgtggt cggcgtgttc caggttgaaa gtcagggcat gcggcgggcg
      601 ctggtcgata tgcggcccga ccgcttcgag gacatcatcg cgctggtggc gttgtatcgt
      661 ccgggtccga tggcgaacat tccgacctat tgcgcccgca aacatggcga cgaagagccg
      721 gaatatctgc acccgatgct cgagccgatc ctgaaggaga cttttggcgt catcatctac
      781 caggagcagg tgatgcagat cgcgcaagtg atggccggtt attcgctcgg cgaagccgac
      841 ctgttgcgtc gtgcgatggg caagaaaatc cgttcggaaa tggaaaagca gcgcgcaggc
      901 ttcgttgccg gcgcgatgaa gaataacgta ccgaaaggcc aggcggatac gatctttgaa
      961 ctactcgcga agtttgccga ctacggcttc aacaagagcc atgccgcggc ctatgcgctg
     1021 gtgtcgtatc acaccgccta catgaaggcg cattatccgg tcgagttcct ggcggcctcg
     1081 atgacgctcg acctcagcaa taccgacaag ctcagcgaat ttcgcgccga agcgcaacga
     1141 ctgggcatca aggtcgaggc gccctcggtc aaccgctccg gcgccacctt cgaggtcagc
     1201 gaaggcacca tctattacgc gctcgccggc ctgaagggcg tcggcgggca ggcggtcgac
     1261 ctgatcgtcg acgcacgaaa agacggcctg ttcacgtcgt tggccgattt cgcagcgcgc
     1321 gtgaatccgc gcgcgatcaa caagcgtgtc atcgagagcc tggcggcggc cggcgcgttc
     1381 gacgcgctgg attccaatcg cgcccgggtg ttcgccggcg ccgaggcgat cctcgcagcg
     1441 tgccagcgca gccatgaggc ggcgaccgtg gggcagaacg acatgttcgg cggtgcggcc
     1501 gacgcgccga ccatcatgct gccgcaggtc gagccgtggc tgccggcgga gcggctgcgg
     1561 cgcgaatatg acgcgatcgg tttcttcctc tccggccatc cgctcgatga ctacgccacg
     1621 gtcctgaaac ggttgcgggt gcagacctgg gcggaatttt cgcgcgcggt caagaccggc
     1681 gcgaccgcgg gcaaggttgc cgccaccgtg gtgtcgcgca tggaacgccg caccaagacc
     1741 ggcaacaaga tgggcatcat ggggctgtcg gatccgaccg gccatttcga agccgtgctg
     1801 ttttccgaag ggctcgcgca gtatcgggaa gtactggagc cgggggctgc ggtgctgctg
     1861 cagctcggcg ccgagctgca gggcgaggat gtccgtgccc gggtgctgca cgccgagccg
     1921 ctggatgcgg ccgccgcgaa aacccaaaaa ggccttcgga tattcgtccg cgataccaag
     1981 ccgctcgagt ccatcgccaa acgcctgcag atgcccgagc ccgctccaca ggggagcccg
     2041 gtccgcggtc ctcagacaaa accgcttccc gacgcggcgg gcggggccaa tggcgatgta
     2101 tcgctggtga tgatgctcga cctcgagacc gaggtcgaaa tgaagctccc gggccgcttc
     2161 agggtctcgc cgcagatcgc cggcgccatc aaggcggtgt cgggggtggt ggacgtgcag
     2221 cagctgtagg cgggggcgtg cgcaccgact agccagcata cagcacgatg tcgaacaccg
     2281 ccaatttgga cttcggctcc atctcaggcg tcggcggcaa caacttggcg atcagcaatt
     2341 cggttcttcc ggcagtttgc agaatgcgat cggtagcgtt gtaggggtcg ccttcgaact
     2401 acatctgggt cacgagtctg tcctgcctgc cggaaacctg gaagtgaatg tgcgcaggcc
     2461 gcattaagtg agggcccacg ggatatgcgg ccggtttgat ggtcttgaac tgatatcggc
     2521 cgtcgtcgtc agcggtcaga atggctgccc cctcaaagtt cggatcgagc ggcgcggggt
     2581 tggtatcgct tggatgcgtg tagcgcccat gagcgttggc ttgccatatt tcaagcttcg
     2641 cattgcgaac aggctcgccg ttcagattga gcacgcgtcc catcacagtg aggacctggc
     2701 cctccgcgcg ccctggccga ccggataccc tcgtcagatc ggcattttga tcaaggggtt
     2761 tcagtggata gaagggacca agtatttgct ccggcgtacg cggcacgacg gttcgcgcca
     2821 tcgcaaacct ggttgagacc acactggcga cgcctgttag tgcggacatc ttcaagatct
     2881 cacggcggct gagatcacct atcgtgcacg tcgccatcgc tgctctccac aaaatccgat
     2941 cggccgaact cgttatcgtg gccgtcaacc gggattcgag ctctgcaggg tccgtcgcgg
     3001 catcaagaga ataccatcaa ggttgtccat cttgaggcgg ctgccgatag ctctgcttcg
     3061 ccgcttgcgt gtcgtgcgca atgccttatt ggattggcag cgcgtttcgt tcaagtgatg
     3121 ttcagctggc catgtgctct atgcgcggat gtcgtagttg aaccgtgagc aggcagggca
     3181 gccatgcgtg tgacaagaag tttttcgatt ctttcgtttt gcctgatcct gttctgcggg
     3241 ttcccgggga tcctgagttc cgccgcagcg cagcagcagg aaaagcggat tgcgctggtc
     3301 gtcggcgacg gcgcttacgc caagtcgccg ctcgcgaccg cggccaacga cgcagggctg
     3361 atcgcgcaaa ccttgcaggc ggccggcttc gacgtggtcg gggcccgcga cctcgacggc
     3421 gacacgctgc gccgaagctt ccgcgacttc atccagaaag ccgaagcctc aggtccggac
     3481 acagttgcgg tggtgtatct cgcaggctac ggaatgcagc tggccggcga gaattatttc
     3541 atacccgtcg actccacggt cagtcgcgac accgacattc caacggaggg aatgcggatc
     3601 aacgactacg tccgccagct tgcatcgctt ccgctgaagg ccggcgtcgt cgtgctggac
     3661 gcggcccggc agcagccttt cgttgagggc ggccagccgc ttgcgagtgg cctcgctctg
     3721 gtcgagccgg atgcgcatat gctgatcgcc ttcaacgccg cgccgggcac gatcgctccc
     3781 accgagcagg gtccgtatgg cgtttacgcg cagtcgctcg cagagatgat ccgcaccggc
     3841 ggcctgtcgc tgcccgaagt tttcaaccgc gtgcggctcc gcgtcaatga attgagcaag
     3901 ggtgcgcagg tcccatggga cgctgaaaag atcgatggac agttcatgtt tttcaatcgt
     3961 gcgccggatg cgcccgcgcc ggcggcgccg gatcaggccg ccgcggttcg cggcaaaccg
     4021 atccgcgacc tcggcgcgca ggatgcctat gccgcagcac tcgaacgcga cacaatgcaa
     4081 ggttatgagg atttcctgac cgcctatccc aacgatccgc tcgccaaacg ggtgcgggca
     4141 atcgccgccg cgcggcgtga agcgattacc tggcttcgct cttaccgggc cgacacgccg
     4201 caagcttact ggtcgtacct gagacgctat ccgcgcggtc cgcatgccgg cgatgctcgt
     4261 cgtcgacttg ccattctcat cgcttcgctg gagccgccgc cgtccttcgc ggtcatcgac
     4321 tacgacgtgc cgccgcctcc gcctgacgaa atcgtctacg tcgaccgccc cgtgctgatg
     4381 ttcagcgacc cggagttcga ctttgcgccg ccgccgccgc cgccactgtt tttcctgccg
     4441 ccgccgccac cggatttcat cgtcttggag ccgccgccgc cgccgatcgg gctgttcatc
     4501 ctgccgcagc cgttcttcgt gccgatcccg gtctacgtcc agcagccggt ctatgtcgtg
     4561 ccaccgccga acaacatcat tttcgccaat atccacaaca cgaccgtcat caacaacgtc
     4621 atcaatcggc cgcctcagcc gggggctggc aacgttcaac ccaatgccaa gggtgtcgcg
     4681 gctccgccag gcgtgaaggg cgcgccgggg cagggcggtg tcgctcagcc cggtaccggt
     4741 ccggcgctgc cgcccgcggt cgcgcaaagg gcctcgctga tccagcaagg caaactggcg
     4801 gtgccgccga gcgcctcgat taatccggca gccagactgg gcactcccgg cacgccatct
     4861 gggcagagag ttgcgccaaa tacgccggtg cccacaaacg tatcccctaa tgcgctgcct
     4921 caagcgttgc cgaagaccaa tgcattgccg gtcccgggtg cgaaggggac accgcctgca
     4981 ccgccaagcg cggcggcgcc ggttccaggt ccgccaaacc ccaacgcgaa actcgccccg
     5041 caagatcagc cgcgggccag cgagccagca ttgcgggcgg gtcaaccgaa agctggcggt
     5101 tcggctcaag ccccggaagc caagccggtc gttccggaaa cggtgcgaag gccaaacgcg
     5161 gctccgccgc caccgccacc acccaaaccg gctgcagcgc ctccaaagcc cgctccgcgg
     5221 gccgagccta agcctcaaat gcaacgggcc gagccgccac atcgggccgc accaccgccg
     5281 cgtccggccc cgcagatggc aaggccatcg cctccgccgc cccgagcggc tccgccaccg
     5341 ccccggatgg ccgcgccgcc gccccctcga cccgccccgc cgcccccgcg gccagcgccc
     5401 cagatcgccc gtccggcgcc gcccccgccg cgaccagctc cacctccgcc acgaccggcc
     5461 gcgccggcgg cggccaagaa atgcccgccg aatgttccaa gatgctgaca gcgaagccgg
     5521 tcagacctca atctgagatc tgaccggacc cgtattgtta acttttagct acagcgagac
     5581 agcgggagaa ttttcttggc aggggacccc taccagctgc cgtcgtgagc ttatgacgcg
     5641 cgttcatcag cggagagact tgcgcgctta ttaggcggcc gaaaggtgtc gatgctgttg
     5701 caaccgtatg gtgcttttgg ttattagctg ccccgtatgg catgggggcg tgatgttttg
     5761 gcgtggggta gtgttgtcgc ttgcggcagc ctcgttggtg ggctgcgcga gcggaagaat
     5821 cggcgctgat tatgcgactg tgacgcagaa ggtcggcccg ccgaaagcag gtcaagcgcg
     5881 catcgtcttg cttcaagaaa aagccagcgg ccttggctat gggagctgcg ccagtgacat
     5941 gaagctcgac ggtgacccga tgggtaggct aaagcccggg acctatcttt atgccgatcg
     6001 tccggcaggt catcaccagc tcctggccgc cgagacaatg tttccaggcg acagcaaacg
     6061 cgacatcaca atggaacccg gccgcacata ttttttcctc gcaaaggtca gcgagaggca
     6121 caatacgctt accgggatga cggtcgcagg tggtctggcc ggcatgctgg tttcctctgc
     6181 cgtcacctct ggcagcgaga atcccgggcc ggtcgacttt tttctcttgg atgaaccggg
     6241 ggcgagggcg acgctcgcgg agcttcaact ggcggaataa tattcgtcgg acagcagctt
     6301 cgacatgaga ggccgcaaac ggcggattca tcggttaggc ttcatcggtt aagcgtctgg
     6361 cgttgatcgg attgggggat ttcttgacta tttcggtcgc caatgtagcg cgcgcgctgg
     6421 ggttgttgct ggtgatcgtg gcgatggacg gcggtgcagc ccgggcggcg ccggatgcat
     6481 cgtcggctgc gcaggccatt tatgccgcgg cgcccccgcg gctcctacag attcgcacgc
     6541 tggtcgctga cgccggacga cagacgtcga ttggctcggg ctttctcgtc tctcccgatg
     6601 ggcttgcgat caccaattat cacgtggtct cgcaggtcgc gctcgagccg aagacctatc
     6661 ggcttgaata tgcggcggcc gatggcagtc atggcgatgt cagcctgtta ggggtcgacc
     6721 tgccaaacga cctcgcgatc attcgcctcg acaagcatga cgtgccttac tttgccttcg
     6781 acgaagctgc gatggcgggc ggcctgccgc agggcgaacg cctctattcc atgggtaatc
     6841 cgcttgacct cggcttcacc atcagcgaag gcacctataa cggcctggtg gggcacagct
     6901 acaacgaccg catccacttc accggcgcgc tcaatcccgg catgagcggt gggccgaccg
     6961 tcaccgccga cggccttatc gtcggcatca acgtcgccac gcgcagaggc ggccagctcg
     7021 tgagcttcct ggtgccggcg cgttttgcgg cggcactgct gcagcgggtt cgcgagcatg
     7081 aaatgccgtc ggacttgcat gccgagatcg cgcgacagct tgccgcgtgg caatccggac
     7141 tctacaaatc gttcggcggg gagggatttc gttcgaccac agtggggccc taccgggcgc
     7201 ccgagacgat ggcgccgtgg ttcgattgct gggcgcagac caacgccggc gccacgccga
     7261 agccgcgcgc cagcatcaat tcgaccaact gcgcgagcga taccgggctg tttgtggccg
     7321 cagatctcaa caccggcgcc atccagctca gtcattccta cgtcaggacc attgacctca
     7381 atcagttcca gcttgcgacc gttctgacgc agctaagcca gccgcgcctg gtcggcggcg
     7441 gaccattccg caagtggtac acgccggagc gttgccacga ggactttgtc agcgccgaac
     7501 cggcgtcgga ccacccgccg ctcagagtga tctggtgcgc gcaggcctat cgcgaattcg
     7561 aggggctcta tgatgtggcg ttgatcgccg tcacccagga ccacggcagc gaggcgctgg
     7621 tctcgcgctt gagcctgcag gcggtcggct atgacgacgc catggcgctc ggcaagcgct
     7681 ttctcgaggc ggtgcaggtg agcaaatgat ctggatcgag atcctgtcgc gccaccgcga
     7741 catcactgcc cgttttcgga ttgccggtcc ggaagcgtac atcgggcgcg gctacgataa
     7801 cgacgtcatc gtcgacgatc cctatgttgc cgcgcagcac ctgcgcatct ttcgcgacga
     7861 gaccgggcaa ctcgtcgccg aggacatggg cagcgccaac ggcctattcc tcgacggcgg
     7921 caagagccga caggcgcgca ttgttgtcga cggcagccat ccgatccgca tcggccagac
     7981 ctttttgcgc atccgcgaga cgagccatgc ggtcgagcgc gagcgcgtgg cgcgaccgga
     8041 gcggcggatt ttcccgatcg tcctggccgc agcactcggc gtggtgatac tgggattcga
     8101 tacggtcaag gtgtggctca cccagacctc cgagccgagg gtttccggct atctgacgcc
     8161 gctgctggtg atggctgcca ccgtgcttgc gtgggttggc gtgtgggcgc tgctgacgcg
     8221 catattctcc ggccattccc gttttctacg caatctgatc attgcacagg tgggcatcct
     8281 cgcgttctca ctctacgacc agggtgcgcg gttctccgcc tttgcatgga cctggtcgac
     8341 ggccagcagc taccaatatg tcgccgcgtg gtcgatcgtt gcggtcgtat gcttcctgca
     8401 tctgcgcgag gtcggcccgg cccgcctgtt gttcaagggc gctctggtaa cgacgttgct
     8461 cgcgactgcg attgcggtgc agacgctgca gcgatcggag gccttttccg actccggccg
     8521 gcaaaccacc gcaagcctgc tgatgccgcc agcgcttcgg ctggccccgc tgcgggacga
     8581 aggcgcattc ttcggcgaaa tcgccaagct caaaacgacg cttgatggcg atcgcaccga
     8641 ggccagggct gaccaggccg gccggtaagt cgcgcaagag ggtcagaatg cgaacgacgt
     8701 tcgtctacgc gcaacgtctg tttcgcgggg accctggaaa agtgccagca gggtggctcg
     8761 gctcgtagtg gccccaggcg gaatcggctg ccggcgggga atgacacgcg atgacagcag
     8821 gcttccacgc caacgccacg cttttcacgg cctgcggcgg gccttcgctg atgccacgcc
     8881 ggctattgcg gggatccgat gtgtatcata gtatgcaact atacacatcg cagggatcgc
     8941 cgatgcgcac taatattgat atcgatgaca cgcttatggc agaagcccag gaggcttccg
     9001 gtcacgccac gaagaagcag acggtggaac aggcgctccg tttgatgatc aggctgcgac
     9061 ggcagcagga ggccagcgcg gctttcggca aataccgctg gcgcggcaat ctcgctcgga
     9121 gccgcaaagg gcgagggatc agttgatcgt cgtcgacagt agcgtctgga tcgacttcct
     9181 caatggccgg aatgcgcccc atgtccggcg acttcgcgca gtcctgggca cggacgaggt
     9241 catcgtcggg gatctgatgc tttgcgaggt gctgcaaggc cttgacagcg agcgggcggc
     9301 tcgagacgtc gaggccctgc tgcggcgctt cgacatagtg ccaatggccg gcgacccgat
     9361 tgccgttgcg gcggcccgca atttccgttc tctgcgccgg cgcggcatca ccgtccgcaa
     9421 gaccatcgac ctgctgatcg gcacctggtg catcgagaac cgcaggccat tgctgcacaa
     9481 tgacagcgat tttcggccga tggcacggca tcttgggctg atcgaagcgc cgatgacagc
     9541 gtgaaggcag gagaacccga gcttgtgcgc tcgggagccc aggggcttac tcaccttggg
     9601 cctccgaaaa acggccctat ttccttgctt ctcgccaaaa tcccgctata taccgcgcca
     9661 tctcacacgg aaacatggct caaaaggccg tccggtggca gccgggccat aaggctcgtt
     9721 tgctcacacg tttccggagg aaccaaccgg agaattttca ctatggcgct tcccgatttc
     9781 tctatgcgtc agctgcttga agctggcgtt cactttggcc accaatcgca ccgctggaat
     9841 ccgaagatgg cggattacat tttcggtgcc cgcaacaaca tccacatcat cgacctcgcc
     9901 cagaccgtgc cgctattgca tcgcgcgttg caggcggtca gcgacaccgt cgccaagggc
     9961 ggccgcgtcc tgttcgtcgg caccaaacgg caggcgcagg acggcgtcgc cgaagccgcc
    10021 aagcgctcgg cgcagtattt cgtcaattcg cgctggctcg gcggcaccct gaccaactgg
    10081 aagacgattt ccggttcgat caagcggctg cggcatctcg atgaagtgct caattccggc
    10141 gatgccaacg cctacaccaa gaaagagcgg ctgaccctgc agcgcgagcg cgacaagctc
    10201 gatcgttcgc tgggcggcat caaggacatg ggcggtctgc ccgacatgat cttcgtgatc
    10261 gacaccaaca aggaagacat cgcgatccag gaggcccagc ggctcaatat tccggtcgcc
    10321 gcgatcgtcg ataccaattc ggaccccaag ggcatcactt atgtcgtgcc cggcaatgac
    10381 gacgccggcc gcgccatcac gttgtactgc gatctggtgg cccgtgccgt gatcgacggc
    10441 atctcgcgcg cacagggcga atccggggtc gatatcggcg cgtcggcgca tcccgtccgc
    10501 gaagaaatca cggtggcgcc gcaacccgcg gccttccagg gtctggccgg gccgcgcggt
    10561 gccgccgaca atctcaagaa gctcaccggc gtttccggcg cgatcgagaa gaagttcaac
    10621 gacctcggca tcttccatta ctggcagctg gccgaactcg accacgacac cgcgcacaag
    10681 atcggcgaag aagtcggact gccaagccgt gccgacggct gggtggccca ggccaaggcg
    10741 atgaccgcgg aagccgaata acgcgaacgc gctggccgga tctttccggc caccaacttc
    10801 ccaatttacg taattgctgg tgatggacgg cggcgaggcg gcaagagccg ccgcggtcac
    10861 cctgacaggc aaaggatacg caacgatggc aacaatcaca gcggcgatgg tcaaggacct
    10921 gcgcgagacc accggcgtcg gcatgatgga ctgcaagcag gcgctcgccg agaacgacgg
    10981 cgacatgcag gcggcgatcg attggttgcg caaaaaaggc ctgtcgaaag ccgcgaaaaa
    11041 ggccggccgc gtcgcggcgg agggcctgat cggcgcgctg accgcaggca ccaagggcgt
    11101 tgtcgtcgag gtcaattccg agaccgactt cgtggcgcgc aacgaacaat tccagggact
    11161 ggtcaagatg atcgcgcaag tcgcgctcga cgtcggcgcc gacgtggaaa agatcaaggc
    11221 tgccaaggtg ggttccgtga cggtcgagac cgccattgcg gacgcgattg cgaccatcgg
    11281 cgagaacatg accttgcgcc gcgccgcttc acttgaagtc ggcaagggcg tggtgtcgag
    11341 ctacgtgcat aatgccgtga tcgagggtgc cggcaaaatg ggcgtgatcg tggcgctgga
    11401 atccaccggc aagaccgacg aactcgcagc gctcggacgc caactcgcca tgcatgttgc
    11461 cgccgccaac ccgcaggcgc tggatccggc cgggctggat cccgccgtcg tcaagcgcga
    11521 aaaggacgtg ctggccgaca aataccgcca gcagggcaaa gccgaaaacg tcatcgagaa
    11581 gatcgtcgag tccggtttga agacctatta caaggaagtc tgcctgctgg agcaggcctt
    11641 catccacgac agcgccaagt cggtgggcca ggccgtgaag gaagctgagg gcaaaat
//
LOCUS       DASWGM010000504        22981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2134070,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000504 DASWGM010000000
VERSION     DASWGM010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 22981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22981
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134070"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VGA15_14405"
     CDS             complement(<1..245)
                     /locus_tag="VGA15_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588923.1"
                     /translation="MMLASWMSFEKPGAMSSVVGCKVFSPGILLGLPFATSPRSCAAS
                     YSSARRPERSCERLVAQDSKGGERDGNLDDDRADRQEP"
     gene            513..758
                     /locus_tag="VGA15_14410"
     CDS             513..758
                     /locus_tag="VGA15_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588924.1"
                     /translation="MVAVDQAANIGIQFQGKRPSGPAFIIWSIPTKRTPADAAGKAGD
                     ADGGRHARQHVKKLGGHRAAIVRVAVSARTKHSKSVE"
     gene            complement(733..1320)
                     /locus_tag="VGA15_14415"
     CDS             complement(733..1320)
                     /locus_tag="VGA15_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588925.1"
                     /translation="MARPRERVCLEQGLKLDLVQLARRGFIQFGANVGPRSIQWNDGT
                     SGEVIASGVITADMSGSDFGWLQINMDNFQQCVELILMPRHFGGGQWYFVCSELNRPA
                     SVLWRPPGALFFRCRQYWGRRAAYLCQFGNPIDRAHIGRAKLKAKLCSPNLEDWRIPP
                     KPPKMRWARYDRLIAQYLKYDAVLGGVPIQQISNA"
     gene            complement(2249..2707)
                     /locus_tag="VGA15_14420"
     CDS             complement(2249..2707)
                     /locus_tag="VGA15_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588926.1"
                     /translation="MTEITIAVSPAFDHRGKRRHDRFDARLQSGDEVMCEATRQPLLD
                     ASRELLRRGADPTATICMVHASAPAVVTMWAPIGVAAQYDVMGEKFVRRKPAAGPMAS
                     SGTGIAMPTAPAEPGDTNPSLRASHSGPLETGSSQLAGIVDHITSSAKGK"
     gene            complement(2704..3126)
                     /locus_tag="VGA15_14425"
     CDS             complement(2704..3126)
                     /locus_tag="VGA15_14425"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEX9588927.1"
                     /translation="MAKSSKTYGAMPATGYMRQSQLIPAIFPFSSATLWRKVKEGTFP
                     KPIKLAPRITAWRVEDIRELLYRGVWAESNHPSPARLGTPSLCTSAEVASRPTTNCDS
                     NVPSLMLESGSENEGRQPQTDSADFASSRLSSGKVRAR"
     gene            complement(3229..4305)
                     /locus_tag="VGA15_14430"
     CDS             complement(3229..4305)
                     /locus_tag="VGA15_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588928.1"
                     /translation="MTGATRVSPLFGYLTASNSAIDPTPKTTRTLYHEFESHSLRHYA
                     LKSLYYFDFLISHPSRNPSIARWLNGEVPLIKKLLNLKQWLTIADAARHLGILFGEDV
                     SEADVLRLALDGHLTLSVYFVNGARGRCGPIVRIEDAQRTIQEENPDDWIHLMGSPPP
                     GEGEKVQVFSSFIHGLSLGDGRVIECAKETELLDGVWDLTMLGHERLEVDQRYQFLTN
                     GPEVDMSFRDGPLDHPIVSREDGTLCQLHEPYFADKHYFPADALPADSVLVVRTSALH
                     DLEKRLAEPDQRAERPVEQRERTSLLAIMAALAQLAKIDVTKPSSAAAAIERQTDLMG
                     ARVAARTIENHLKLIPEVLERLSK"
     gene            complement(4596..5591)
                     /locus_tag="VGA15_14435"
     CDS             complement(4596..5591)
                     /locus_tag="VGA15_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003569764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9588929.1"
                     /translation="MATIADVARVAGVSMSTVSHVLNGTRFVSPETEQVVRDAISQMG
                     YTPNTLARALAKSTSNSVGIAISGVSNPYFNDIIRAVERECASFGMTVFLADTHDTPM
                     KELEVVQALHQHRVDGIILAPCSDTKQSALAYLRENQIPTVLVDRLASKAFDQVGVSN
                     AKSVERLVDHLVGHGHRRIGMLPGQPGFATTIERVEGFQKAIRRNRLDPDRCPVAIGS
                     IDVESAARRVEALMSGANPPTALLAGNNMTSIGAMRGLKRMKLVVPRDMALVGFDDFE
                     WADCFEPQLTVIAQPCLEIGRSAAKMLLERIKKPDREPKIVRHKTELIIRNSCGC"
     gene            complement(5756..6853)
                     /locus_tag="VGA15_14440"
     CDS             complement(5756..6853)
                     /locus_tag="VGA15_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9588930.1"
                     /translation="MAEVTLERVEKRYGSYTVVPHLDLKIDDGEFAVLVGPSGCGKTT
                     TLQMMAGLETISSGRLFIGGRDVTVLPPKERDIAMVFQSYALFPHMNVRDNINFGLKI
                     RRTPAAEMQTMVSEVARRLQIDSYLDRLPKELSGGQRQRVALARALVRRPGVFLMDEP
                     LSNLDAKLRVEARSFLSKMHHEIGTTTVYVTHDQSEAMTMGSRIVVMKGGVIQQAAPP
                     LEIYRKPANRFVAGFIGSPSMNFLELEVAEGALVDGAAGIRIPLPDSRSAEVARSGSP
                     TVVLGVRPEHLHLLADGEGGQYTTVFQVEVVQHLGNEMLLDIVAGPHRAIARADAEAR
                     VREGERRTFSIDMDRAHFFHPRTEVNLAIAG"
     gene            complement(6890..7747)
                     /locus_tag="VGA15_14445"
     CDS             complement(6890..7747)
                     /locus_tag="VGA15_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9588931.1"
                     /translation="MSDVAFMMRQRSLVAGKLIGLMLLAALVFVWLGPIILVVITSIK
                     SNDAFLAGPFALPTEPTFAPYVTVWNALGFGRLLANSFLYATAGSALAVIPALVPSFA
                     LSRFDVPGKKLVFGIILTGLMLPQQTVLIPLYDTLRALSLLDTRIGLIIVHAAYGMPA
                     QILILRGFMTTIPREIEKAAFLEGATDFQVFWKVILPLSVPGIMVGFTLNFIAIWKEF
                     VFGLVLLNSEANFPVTVGMLKLNSDRYMAVFNLPAAGLVISQIPIVILFLLSYRKLSS
                     GNFAGAVKG"
     gene            complement(7740..8669)
                     /locus_tag="VGA15_14450"
     CDS             complement(7740..8669)
                     /locus_tag="VGA15_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9588932.1"
                     /translation="MAHPSLADERTSFLRFAHTHKFVTFFGILVPLAVFAVFVGYPIV
                     FTIYLSFFDWNGMAAEKTFVGLQNYRYLLVSDKYFYTALLNNVKWVGVTLFFPVLLGL
                     IIAYGLRSRIIPVPALIRTVIFFPVTMSLISVGLMFLLILNPLFGAFDAILQSIGLGS
                     LVMEWFGDHRIALYTLAVVSGWAFTGMPMILYFAGLGDVPKETFDAAKIEGAGHFRML
                     TQVAVPQLRPVTAVVVMLTLFESLRAFDLVAVMTKGAPFGSTNVLGYLVYLESFWNSR
                     FGYGATLSVAILVISILMATFVMKKLMKGALDV"
     gene            complement(8803..9261)
                     /locus_tag="VGA15_14455"
     CDS             complement(8803..9261)
                     /locus_tag="VGA15_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9588933.1"
                     /translation="MFEPSFNSIDIGPAMIPWSQSKALHWYQGSFNLGRFRGEKEKCC
                     VVPMDFFPLPPIDGNKPVMSVFAEDTIMIHAKSPNKDAAAEFVNWMISKPAMTEKLVI
                     DKPYPSSTEADLARLSEMEQRLAKAMVDAGSFTFSGSGRRHDAGGCHAAN"
     gene            complement(9216..10019)
                     /locus_tag="VGA15_14460"
     CDS             complement(9216..10019)
                     /locus_tag="VGA15_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9588934.1"
                     /translation="MGGVTASNRIFSRRHFVAAALAVGSLAATLGGIAPAFAAPIEIR
                     YFYRAPWPSSETYANWLINEWNKANGDRIHVTGACVDGETYKTKQTIELASSNPPDVF
                     YSWEGGRAAEVIKNGFAADLTPYYAKYGWDKFLVPAGVSLATFGDRKYFVPTELGASV
                     VWYRKDLYEKLGLKVPTTWDQLMANAKVAKDNGYWPFMLANQKKWPSQFMWSAILVNK
                     FGLDTYQGLIDNKIPWTDQRAVATTKIMQDLAKAVCSSRPSTRSTSALP"
     gene            complement(10788..12614)
                     /gene="edd"
                     /locus_tag="VGA15_14465"
     CDS             complement(10788..12614)
                     /gene="edd"
                     /locus_tag="VGA15_14465"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEX9588935.1"
                     /translation="MIIEATVNARVAEVTKRIARRSSDKRARYLDRIAEAAATPHRQR
                     LGCANQAHAFAACAPRDKTMMREGRVPSLAIVTAYNDMLSAHQPYERFPELIRRSARE
                     AGAVAQVAGGVPAMCDGITQGEAGMELSLFSRDVIALSTAVALSHQTFDAAVFLGICD
                     KIVPGLVIGALSFGHLPAVFIPSGPMTSGLANDAKSKIRQAYAEGKVGREALLEAEAK
                     AYHGPGTCTFYGTANTNQMLMEIMGLHLPGASFVNPNTPLRDALTRAATERALAMTSL
                     GNDYTPLGRMLDERAFVNGIVGLHATGGSTNHTLHLVAMAAAAGLTLNWNDFADLAEV
                     TPLLSRIYPNGSADVNHFHAAGGMSFLMRELVGAGLLHGNVSTVCGKSLAAYVQEPML
                     DAAGALAWRDGPAASGDASVLRGVAEPFQATGGLTVLDGDLGRAVIKTSAVARERLVI
                     EAPARVFHSQEELQKAFAAGELTSDMVAVVRFQGPKANGMPELHKLMPPLGALQDRGL
                     KVALVTDGRLSGASGKIPAAIHVTPEAVEGGAIGKIRDGDLIRLDAVAGRLNVLVERH
                     EWNARVPAQADLSAAQAGVGRELFALFRDRVGRADVGASIFA"
     gene            complement(12722..14200)
                     /gene="zwf"
                     /locus_tag="VGA15_14470"
     CDS             complement(12722..14200)
                     /gene="zwf"
                     /locus_tag="VGA15_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX9588936.1"
                     /translation="MTSRVIPVAPFVLTVFGATGDLARRKLLPALFHRDFDGQLPEAA
                     TILGAARRPMSRDQFVALARDAIVEHIPASQTGGPELDRFLARLLFFAIDAEGEDGWA
                     ELVAAMAPFAGRVQVYYLATAPQLFGPICKRLGRHGLSGNEARVVIEKPIGKDLASAI
                     SVNQAIGKVFPEERVFRIDHYLGKETVQNLMALRFANALFEPLWNAAHIDHVQITVAE
                     AIGVEGRGAYYDSSGALRDMVQNHLLQLLCLVAMEPPNSLEADAVRDEKLKVLKSLAR
                     IDDSNAAALTVLGQYRAGASNGVAVPGYADEIGHAEGRTETFVAIKAAIANWRWTGVP
                     FYLRTGKRLQQRCSEIIVTFRKVPHSIFDKETGRISQTRLVIRLQPDEGIRLWLMIKE
                     PGPGGMRLQHVPLDMSFAKAFDVSVPDAYERLLMDVVRGNATLFMRRDEVEAAWRWID
                     PIREAWNRMNEVPRSYVVGSWGPSAATALIERDGRTWMEDSP"
     gene            complement(14197..15195)
                     /locus_tag="VGA15_14475"
     CDS             complement(14197..15195)
                     /locus_tag="VGA15_14475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX9588937.1"
                     /translation="MILVCGEALIDLFVGPAEGSEMPARAVAGGSPFNVAIGLARLGV
                     KAGFLGGISRDHFGALLAGILVHEGIDDRFLVRTDRLSTISMVATDDNGQPHYAFHGE
                     GAADRSLGLADLPASLPLDVRALTFGSYTMAVEPVGSAFAALAERECGRCVISVDPNL
                     RPTVVGDMARWATAAERFYRTATIIKASDEDVRAAGGGRVSVVEAAVYWLNCGARLVV
                     VTEGAKGATAFCAAGHVSVPAHSVVVRDTVGAGDTFHAALLAQLAKTDKLDPDAIAAL
                     DLPAIRDLLGYAMAAASVTVSRNGADLPTEADVAANLAAVGRLSENSVIDSRKGLA"
     gene            complement(15267..16511)
                     /locus_tag="VGA15_14480"
     CDS             complement(15267..16511)
                     /locus_tag="VGA15_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX9588938.1"
                     /translation="MDEGAPFAGASQPMTALSAGSRGTSQSGVGLYNERLILSLIRRQ
                     RSLAKVEIARLTGLSTQTTTVIINRLEADGLLVAGEPQRGRIGQPSVPYTLNPDGAFG
                     LGLMIGRRSSDLVLMDFTAGIRARRRMIYPYPAPAKILAFAEREMPDLLAELPVAQHG
                     RISGLGVAMPFELWNWEADLETPPGALGQWRHFDVAAELSRRFARWPVRLCNDATSAC
                     AAELTFGAGATYRDFAYFYVGTFIGGGIVLNGRLFPGRSGNAGALGSMPIMRREPNGD
                     ASLQQLIRTASIYRLERRLIAAGRRGTDIWLSPDDWSNFAEPLDVWISEAAEALAQAI
                     TSLLAVIDFEAIVIDGAFPANVRERLVAQTRAQRARIDQQGLTDAVVVEGSIGRDARA
                     IGGAALPLLAAFAPDHDVLFKD"
     gene            16787..17806
                     /locus_tag="VGA15_14485"
     CDS             16787..17806
                     /locus_tag="VGA15_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9588939.1"
                     /translation="MRQPLVFFAALGIALRVTAANAADKPIVGLITKTNTNPFFVKMK
                     AGAEDAATANGIELRSFAGKVDGDNDGQVAAVENLIATGAKGILITPNDSRAIVPAID
                     KARAAGILVIALDTPLDPPTAADATFATDNFLAGELIGQWAAKTLGPKAASAKIALLD
                     LNVNQISVDVARDQGFLKGFGIDLGNPAKIGDEKDARIVGHDVTLGSEEGGRKAMENL
                     LQKDPGISVVYTINEPAAAGAYEALRSVGHDKDVLIVSVDGGCPGVHNVKDGVIGATS
                     MQFPLLMAAKGVEAVKKFAETGTKPQPSPGHDFFNTGVELVTDKPAAGLTSIDSAAAL
                     KKCWG"
     gene            17922..19019
                     /locus_tag="VGA15_14490"
     CDS             17922..19019
                     /locus_tag="VGA15_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588940.1"
                     /translation="MTDIGNSHPAIQALEVQLDRKELDKKDDRVVEFEVRAKSPLRKL
                     QHFLHANPTAVPAIVLFLGIAAFGLIVGSRFLSSFNLSLIIQQVTIIGVIGVAQTLIV
                     ITAGIDLSVGAIMVLCSVIMGKLAVTAGLPAQLALLIGILAGGGCGAVNGILVTRFKL
                     PPFIVTLGTWSIFFALNLWYSASETIRSQDVAKTAPFLQALGTPVDILGARFTYGSFF
                     MLALVGLIWFVLNRTAFGRHLYAVGDDPDAARLAGIRTGRVLFTVYVVAGVICALGAW
                     ALIGRIGSISPQAGQTANLDSITAVVVGGASLFGGRGSIVGTLVGALIVGVFRNGLAL
                     SGVDVLWQEFTVGWLIIVAVALDQWIRKVSA"
     gene            19016..19825
                     /locus_tag="VGA15_14495"
     CDS             19016..19825
                     /locus_tag="VGA15_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9588941.1"
                     /translation="MSNDGQTFKSTSASTPILQARGLAKRYGRVVALDNADFDLLPGE
                     ILAVIGDNGAGKSSLIRALSGALTLDAGEIRLNNQPVTFRSPLDARAASIETVYQTLA
                     LSPSLSIADNMFLGRELRRVGPLGSVCRMLDRKAMQRIAREKLTELGLLTVQNINQRV
                     ETLSGGQRQGVAVARAAAFGSRVVIMDEPTAALGVKESRRVLELILDVRRRGVSIVLI
                     SHNMPHVFEIADRIHIHRLGRRLTVIDPKSYTMSDAVSLMTGARLPVEIAA"
     gene            complement(20131..20505)
                     /locus_tag="VGA15_14500"
     CDS             complement(20131..20505)
                     /locus_tag="VGA15_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588942.1"
                     /translation="MVLFASWRLVCLHEMAALVHYSLFRFELLACHLTIDQASKRPEG
                     LVCADGNDRSVIDPNLFVPLRHAHVSAPQIQTVHLSPTPLLKLGRIWSKSITGFQVDP
                     EQTFQYPLNIGVLEHENLAAAY"
     gene            20582..20755
                     /locus_tag="VGA15_14505"
     CDS             20582..20755
                     /locus_tag="VGA15_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9588943.1"
                     /translation="MTTTTILRFRRKLGAFQQRARCEPIEITRHGRRAFVLMSAEHCD
                     WMQGCQPADVSCF"
     gene            complement(21304..22608)
                     /gene="ltrA"
                     /locus_tag="VGA15_14510"
     CDS             complement(21304..22608)
                     /gene="ltrA"
                     /locus_tag="VGA15_14510"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX9588944.1"
                     /translation="MTKASIDLQDLRRRIYVKAKAEPSWRFWGLYVHVCKMETLREAY
                     KMAKKNDGAPGVDGVTFEAIEAQGAEALLEQLRDELIGRTYKPLPARRQEIPKDGGKV
                     RVLSIPAIRDRVVQGALKLIVEPVFEADFQPGSFGYRPKRTAHDAIKRVAEAIVQRKT
                     RVLDFDLRSYFDNVRHDRLLEKVAKRINDADIMHLLKVMLKASGKRGVPQGGVISPLL
                     SNLYLNEVDRMLERAKETTHQGKYTYIEYARFADDLVILVDAYKRHDWLIGAVEKRLR
                     EEFDKLQVEINDEKSRTVDLGRGESFGFLGFDFRYLRSVHSGAWRPHYTPKLKKRTAL
                     LRELKEVFRRHRSQPIERVISLINPVLRGWVNYFAVGHSSECFSFIKDWMEKKIRRHL
                     AHAQKRKGFDWERWNRRWLYDTLGLFNAYRVRRDGPKVVPTG"
BASE COUNT         4466 a         7134 c         7028 g         4353 t
ORIGIN      
        1 ggctcctgac gatcggcacg atcatcgtcg agattcccgt cgcgttcgcc gcctttacta
       61 tcttgagcca ctaagcgctc gcaggaccgc tcaggccgcc tcgccgacga atacgacgcg
      121 gcgcaggaac gtggtgaggt cgcaaagggt aggcccaaaa gtattcccgg cgagaatact
      181 ttgcagccga caaccgatga cattgcgccg ggcttctcga aagacatcca cgaagccagc
      241 atcatccgcg atgccgaagt cgccgaccct ggcatcgtgc gtcgcgcaaa cgaggcgcaa
      301 acccgcgacc gcgggacgaa cggcccggcg cttaccggga agatgatgca ccgggccgtt
      361 gccccaggtc ccggctatag cgctgcaatt gccatgccgg ggtcgcgtca gggtgccgcc
      421 cggtcgccac cgcctcgacg agccgcgcca ccgctttttc tcgggttttt gaccagcaga
      481 ctgctgccga cgtagcccaa atgaagggcc agatggtggc ggtcgaccag gccgccaata
      541 tcgggatcca atttcagggt aaacgcccgt caggacccgc tttcatcatt tggtcgatac
      601 cgacgaagcg cacgccggcg gatgcggcag gcaaagccgg cgatgctgac ggaggtcgac
      661 atgcgcgcca gcatgtcaaa aagctgggcg gccaccgcgc ggcgattgtc cgcgtcgctg
      721 tttccgcaag aactaagcat tcgaaatctg ttgaataggt acaccgccaa gcacggcatc
      781 atacttgagg tattgcgcga taagacggtc gtacctggcc caacgcatct tgggcggttt
      841 cggcggaatc ctccagtcct ccagattcgg tgaacagagt tttgctttca atttggccct
      901 accgatgtgg gcccggtcga tcggattgcc gaattgacaa aggtaggcgg cccgtcggcc
      961 ccaatactgg cggcaccgga aaaatagtgc tccaggcggt cgccagagaa ctgaggcggg
     1021 tcgattcagc tccgaacaca caaaatacca ttgcccgccg ccgaaatggc ggggcatcaa
     1081 aataagctca acgcactgct ggaagttatc catgttgatc tgcaaccatc cgaaatccga
     1141 accgctcatg tctgcggtga tgacgccgga tgcaattacc tctccggacg tcccgtcatt
     1201 ccattggata cttcgcgggc ccacgttcgc gccaaactgg atgaatccgc gccgcgcaag
     1261 ttggaccaga tccagcttta agccctgctc gagacaaacg cgttcgcgtg gacgtgccat
     1321 tctagggcgg cctccctcag tgtgccaatc acacccccca agccagacac aaggcggcag
     1381 cggcgaatgg cagcgccgac ggtgctactg ccgccccgcg tccgccagca ttcgcctgcg
     1441 taccggcctc gctgctttta tttatttatt cccgtcaact cgcgttgacg aggagttcga
     1501 caaattctcc ccggcagaac tttccgtagc cggcagggcc cttccgggtc ggccatgagt
     1561 tatcagtgtt gatggccttg gagacaataa tgatgttgca gcgataagct ggctataggc
     1621 cctatgccag ccccttacgt tgcttcgaat ccttcggcag ggggaggaag gccggacgcg
     1681 gcatgaaccg cgttcagcga ggctgccata ggggacggga gctcccatca gattttctct
     1741 caccagcggg tctccctgcc cctccacgtt gtggctcgaa tggcgtggtc ggtaatcttt
     1801 gcgctgagct gatcgtttgg cgttcgtgtg cttccatggg tcgaccaagc cggcctcaat
     1861 ggtgtcgcgc tcgagctgtc gggaaagacg gacacgggaa ccacaatttc aaggcgcgcg
     1921 cggcgagaca ccgccgtcac gttctggcta ttcacgccgg ttcgtcgtgc tgccgccatg
     1981 gaagatgcag gccgacactt tctcgagttt catcgaggcc atgtagggcc gcaaaagggt
     2041 ttggaaggca ggtaaggcgc tggacgtgcc gtgcagccgg catcaacgtg gcgacgtcac
     2101 ggcggtaggc ccacatgccg agaggggtga gatcaaaatc ggcgtgactt cgcacccgat
     2161 gacgcggtat tctttgcata gttgatcctt tgctttgcgg tggaggagcg acgtttaggc
     2221 cggcgtgagg agcaactcat gccggccttt acttgccttt ggcggagcta gtgatgtggt
     2281 cgacgatgcc ggcgagctgt gagctccccg tctccagtgg gccactatgc gaggcccgaa
     2341 gagaggggtt tgtatcgccg ggctccgcag gggcagttgg catggcaatt ccggtccctg
     2401 aactcgccat cggccctgca gccggctttc tcctgacgaa cttctcaccc atgacgtcgt
     2461 attgagcggc gacgccgatc ggtgcccaca tcgtcacgac agcaggcgcg ctggcgtgga
     2521 ccatacagat cgtggcggtt ggatctgcgc cacgtcgcag cagctcgcga ctggcatcta
     2581 gcaggggctg ccgggtcgcc tcgcacatga cttcgtcacc gctttgaaga cgggcatcga
     2641 agcggtcgtg acggcgcttg ccgcgatgat caaatgcggg cgagactgca atggtaatct
     2701 cggtcatcgc gcgcggacct tgccagagga caatcgggag cttgcaaagt cggcggagtc
     2761 tgtttgaggc tggcggcctt cgttttcact gcctgattct agcatcaacg acggcacatt
     2821 cgaatcgcag ttagtcgtcg ggcgcgaggc cacctccgcg gaggtgcaaa gactcggcgt
     2881 acctaaccgg gctggactgg ggtggttaga ttcggcccaa accccgcggt agagcaactc
     2941 gcgaatatct tcgacgcgcc acgcggtgat gcgcggcgct agcttgattg gcttagggaa
     3001 ggttccctcc ttgactttgc gccacagcgt tgccgatgag aacgggaaaa tcgcggggat
     3061 cagttgggat tggcgcatgt agccggtggc cggcatcgcg ccatacgttt tggaactttt
     3121 tgccatgtcg cgtgacctcg tgaagtgggt tggtgcgaca tggacaagat agcgactcgc
     3181 cgtgcgaatg acgcaattgc ggacccgagt tacgcaattg cggaaaattt atttgctgag
     3241 acgctcgaga acctcgggga taagtttcaa atggttctcg atcgtgcggg cggcgacccg
     3301 agcacccata aggtcggtct gcctttcgat cgcggcggcg gcgctggacg gcttcgtcac
     3361 atcgatctta gccagctgcg ctagggcagc cattatcgcc aacagagacg tgcgctcgcg
     3421 ctgttcgaca ggcctttcgg ctctttggtc cggctccgcc aaccgctttt ctagatcgtg
     3481 aagtgccgaa gttctgacaa ctaaaacgct gtcggcagga agggcatcag caggaaaata
     3541 atgcttatcg gcaaagtacg gctcgtgtaa ttggcacaag gtgccatctt cacgagatac
     3601 gattggatgg tctaacggac cgtcccggaa ggacatatcg acctcgggac cgttggtcaa
     3661 aaattggtaa cgttgatcga cttccaaccg ctcgtgaccc agcatcgtga gatcccaaac
     3721 tccatccagc agttcagttt ccttggcaca ctcaattacc cgaccatccc cgagcgaaag
     3781 accgtgaatg aagctggaaa atacttggac tttttcgcct tcgcccggcg gcgggctacc
     3841 catcaagtgg atccaatcgt caggattttc ttcttgaatc gtccgttgtg catcctcgat
     3901 acggacgatg ggtccacatc ggcctcgggc gccgttaacg aaatagaccg agagcgtaag
     3961 gtgaccgtcc agcgcaagcc gcaacacatc ggcctcgctc acgtcctctc caaatagaat
     4021 gcccagatga cgagctgcat ccgcgatggt cagccattgt ttaagattga gtagcttttt
     4081 aattagcggc acctcgccat tgagccatct tgcaattgat gggttgcgcg atgggtgcga
     4141 tatcagaaag tcgaaataat ataatgattt caatgcataa tggcggagag agtgggattc
     4201 gaactcatga tacagagttc gcgtagtttt cggcgtcgga tcaatggctg aatttgatgc
     4261 cgtcaaatac ccgaataaag ggctgacgcg cgtcgcgccc gtcattctcg atggggccta
     4321 gtaacgctgc aaacgcagcc ggaccatttg tcgcttggct ccgcgttccc cagaaaagac
     4381 agatccgtcc cggcggttag accgggacgg tgagtgagct ataacaaaag tggttgcggg
     4441 gaggcgcaac caccgatacc gacactcgct gatggtgacc gtctgaaacg gacggtgtac
     4501 tgtgtcccga acgcagtgaa ataccatgcg ccggtcgagg ccagacacaa tgcaacgaac
     4561 ttccggttca ggacaccaga tcccacccac acgacttagc agccgcacga atttcggatg
     4621 atgagttccg ttttgtgtct gacgatcttc ggctcccgat ccggcttctt gatccgctcc
     4681 agcagcatct tggctgcgct gcggccgatt tccaggcagg gctgcgcgat cacagtcaat
     4741 tgtggttcga aacaatcggc ccattcgaaa tcatcgaagc ccaccagcgc catgtctcgg
     4801 ggcacgacga gtttcatgcg cttcaacccg cgcatggcgc cgatcgacgt catgttgttg
     4861 cctgcgagca gcgccgtcgg cgggttcgcc ccgctcatca aggcctcaac gcggcgcgcg
     4921 gcggattcca cgtcgatcga gccgattgcg acggggcatc ggtccgggtc caggcgattg
     4981 cgacggatcg ccttctggaa gccttcgacg cgctcgatcg ttgtagcgaa tcccggctgg
     5041 cccggcagca tgccgatccg ccgatgcccg tgcccgacca gatggtcgac aaggcgttcg
     5101 accgatttgg cgttgctgac accgacctgg tcgaaagcct ttgacgcgag ccggtccacg
     5161 agtacggtcg ggatctgatt ctcgcgcaga taggcgagcg cgctctgctt ggtatccgaa
     5221 caaggcgcca ggatgatgcc gtcgacgcgg tgctgatgca gcgcctgcac cacctccagt
     5281 tccttcattg gcgtgtcatg ggtatctgcc aggaagacag tcatgccaaa gctggcgcat
     5341 tcgcgttcca ccgcgcgtat gatgtcattg aaataggggt tagaaacgcc cgagatcgcg
     5401 atgccgacgc tattgctggt ggatttcgcg agcgctcggg ccagtgtatt gggcgtgtag
     5461 cccatctggc tgatggcgtc gcggaccact tgctcggtct cagggctaac aaagcgcgtt
     5521 ccgttcaaca catgcgaaac tgtcgacatg gagacaccgg ccacccgcgc cacgtcagct
     5581 atcgtagcca tgaaattcgc ttccctgacg agccgtaacg cgtgcggagg tctcgcgccg
     5641 tttccagtgc ccccttcccg cgatcaaaca aaacgcatga ctctgcgcgt gagttagacg
     5701 aatggccccg ctcgaacgcg tgccgaaaca cggcggcata gtaaagagga tgccatcacc
     5761 ccgcaatcgc cagattgacc tcagtccgtg gatggaagaa gtgcgcgcgg tccatgtcga
     5821 tactgaaggt ccgtcgctcg ccttcgcgga cccgcgcctc cgcgtcggct cgcgcgatcg
     5881 cgcggtgggg gccggcgacg atgtccagca gcatttcatt gccgagatgc tgcaccactt
     5941 cgacctggaa gacggtcgtg tattgcccgc cttcgccgtc cgcaagcaaa tggagatgct
     6001 ccggccgcac accgagcacg accgtggggc tgcccgaccg ggccacttcg gcggacctac
     6061 tgtcgggtag cggaatccgg atcccggcgg cgccatccac gagcgcgcct tcggcgacct
     6121 caagctccag aaagttcatc gacggcgatc cgataaagcc ggcgacgaag cggttggctg
     6181 gtttgcgata gatttccagg ggaggcgccg cctgctgtat cacgccgcct ttcatgacga
     6241 cgatccggct gcccatggtc atggcctcgg actggtcgtg cgtcacgtag accgtcgtcg
     6301 tgccaatctc atggtgcatc ttgctgagga aactgcgtgc ctcgacgcgc agcttggcgt
     6361 cgagattgga cagcggttcg tccatgagga agacgccggg ccgccgcacc agcgcgcgcg
     6421 cgagggcgac acgctgccgc tggccacccg acaattcctt aggcaaccga tcgagatagc
     6481 tgtcaatctg caggcgtcgc gcgacctccg agaccattgt ctgcatctcg gcggcgggcg
     6541 tccggcggat cttgagtccg aaattgatgt tgtcgcgcac attcatatgc ggaaacagcg
     6601 cgtaggactg aaacaccatc gcgatgtcgc gctccttcgg cggcagcaca gtgacgtcgc
     6661 gaccgccgat gaagagcctg cccgaagaaa tggtctccaa gcccgccatc atctgcagcg
     6721 ttgtggtctt accgcagccg gatggcccga ctagcacggc gaattcgcca tcgtcgatct
     6781 tcagatcgag atgcggaacg acggtatagg aaccgtaccg cttttccacc ctttcgaggg
     6841 tcacttcggc catggattat gccttttctt gataccagag acaccagagt cagcccttga
     6901 ccgcgcctgc gaaattgccc gacgagagtt tgcggtagga gagaaggaag agaatgacga
     6961 tcgggatctg cgagatcacg aggccggcgg ccggcaggtt gaacactgcc atgtaacggt
     7021 cgctgttcag cttcagcatg ccgacggtca ccgggaagtt cgcctcagaa ttgaggagta
     7081 ccagcccgaa gacgaattcc ttccagatgg cgatgaagtt gagcgtaaag cccaccatga
     7141 ttcccggcac cgacaaaggc aggatcactt tccagaacac ctgaaagtcg gtcgctcctt
     7201 cgaggaaggc cgccttttcg atctcccgcg ggatggtcgt catgaagccg cgcagaatga
     7261 ggatctgggc tggcattcca taagcggcgt ggacgatgat aaggccgatc cgggtgtcga
     7321 gcaggctcag ggctctgagc gtgtcataga gcgggatcag cacggtctgc tgcggcagca
     7381 tcaacccggt caggatgatt ccgaagacga gtttcttgcc cgggacgtca aagcgggaca
     7441 atgcaaagga cggcaccagc gccgggatca cggccaacgc cgagccggcc gtcgcataga
     7501 ggaagctgtt cgcgagaagt cggccaaacc caagagcatt ccagaccgtg acgtatggcg
     7561 cgaatgtcgg ctccgtcggc agagcgaacg gcccggccaa gaaggcgtca ttggacttta
     7621 tcgaggttat gacgacgagg atgatcggac cgagccaaac gaagaccagc gcggcgagca
     7681 gcatcagccc gatgagcttg ccggcgacaa ggctcctttg acgcatcatg aatgcgacat
     7741 cagacatcga gggccccctt catcagcttc ttcatcacga aagtggccat gaggatggag
     7801 atgacgagga tggcgaccga tagcgtcgcg ccatagccga agcgggaatt ccagaagctc
     7861 tcgagataga cgagataacc gagcacgttg gtggaaccga agggggcgcc cttggtcatc
     7921 accgcgacga gatcgaaagc gcgcagcgat tcgaataggg tgagcatgac gaccaccgcc
     7981 gttacgggcc tgagttgggg tacggcgacc tgcgtcaaca tacggaaatg accggcgccc
     8041 tcgatcttgg ctgcgtcgaa ggtttctttc ggaacatcgc cgagcccagc gaaataaagg
     8101 atcatcggca ttccggtaaa ggcccagccg gacacgacag ccagcgtata cagggcgatg
     8161 cgatggtcgc cgaaccattc cataacgaga gagccgaggc cgatcgattg aaggatggcg
     8221 tcgaacgcgc cgaacagcgg attgaggatc agcaggaaca tcaatccgac ggagatcagc
     8281 gacatggtga ccggaaaaaa gatcaccgtt cggatgagcg ccggcaccgg aatgatccgg
     8341 ctcctgaggc cataggctat gatgaggccg agcaggacgg gaaaaaacag cgtgacgccg
     8401 acccatttca cattgttgag cagcgccgtg tagaaatact tgtcgctgac cagcagatag
     8461 cgatagttct gcagtccgac gaaggtcttt tccgccgcca tgccgttcca atcgaagaaa
     8521 ctgagataga tcgtgaagac gattggatag cccacgaaga cggcaaagac ggccaacggc
     8581 accaggatgc cgaagaacgt cacgaattta tgagtatgcg cgaagcggag gaacgaggtc
     8641 cgttcatcgg caaggctggg gtgcgccatt gcaaggatcc aaattcggga ggccgctgaa
     8701 ttcaggaacg agcacgcgcc gcgaggaagc gataatctcc ctcgcggccg ttcgtccggt
     8761 tcgcccggct agatcttgcc gcgcacgcga gcagcttcat tctcagtttg ctgcatggca
     8821 tcctccggcg tcatggcgcc ggccagaacc gctgaaggtg aaggatcccg catccaccat
     8881 cgccttggcg agacgttgtt ccatctccga taggcgcgcc agatcagcct cggtcgaaga
     8941 gggatacggc ttgtcgatga caagcttctc cgtcatcgcc ggcttcgaga tcatccagtt
     9001 gacgaattcg gctgctgcgt ccttgttcgg gctcttggca tgaatcataa ttgtatcttc
     9061 cgcgaagacc gacatgacag gcttgttgcc gtctatggga ggcagcggga agaagtccat
     9121 tggtacgacg cagcactttt ccttctcgcc acggaaacgg ccgaggttga acgatccctg
     9181 ataccaatgg agcgccttgc tttgcgacca tggaatcatg gcagggccga tgtcgatcga
     9241 gttgaaggac ggctcgaaca taccgccttg gccaaatcct gcataatctt cgtcgtggcg
     9301 accgcccgct gatcggtcca cggaatcttg ttgtcgatca ggccttgata ggtgtcgagg
     9361 ccgaacttgt tgaccaggat tgccgaccac atgaattgcg acggccattt tttctggttg
     9421 gccagcatga acggccaata gccgttgtct ttagccacct tcgcattcgc cattagttgg
     9481 tcccaggtcg tggggacctt gaggccgagc ttctcgtaga gatccttgcg ataccagaca
     9541 accgaggcgc caagttcggt cggaacgaag tactttctgt caccgaacgt cgcgagcgaa
     9601 acgcctgccg gaacgaggaa cttatcccag ccgtactttg cgtaataggg tgtcagatcg
     9661 gcagcgaagc cgttcttgat gacttcggca gcgcggccac cttcccagga ataaaacaca
     9721 tccgggggat tgctagaagc aagctcgatg gtctgcttgg ttttgtaggt ctcgccatcg
     9781 acgcaggcgc cggtgacatg gattcggtcg ccattggcct tgttccattc gttgatcagc
     9841 cagttggcat aggtttccga agacggccac ggcgcgcgat agaaatagcg gatctcgatc
     9901 ggagcggcga atgccggcgc gatcccgccg agggtcgcgg caagtgagcc gacggccaaa
     9961 gccgccgcca cgaagtggcg gcgtgaaaaa atgcgattgg atgctgtgac tcctcccatg
    10021 acacgtcgtt tctggacgct taccgcgctc gaacgaacga ttgatctgtg attgcttatt
    10081 caggaatgca cttctgttgt tcaggtcttc cgcggcgagc tatttgcgaa atccccgtgg
    10141 acgggcaccg aggcccgtca cccgctcacc acctccctct tccggactga tttcatctat
    10201 ggcggctgcc gacactacca catcgaagcg cttgcgcaag cgcttcgata gcataaacct
    10261 gaagttatgg gcgcgcaatg acttgagttg cttcagcttc aacggattct ctgaaggcaa
    10321 aatttgcgga aaaaagacga aatccgctgt cgccgcctct ccgcaacaga acgtcgagtc
    10381 gaagttgcca ccggcgatga ttttgccaga ctcaaggcgg cgcgcggccc tatcggacta
    10441 gatgcgaatt accgcggcag gcggttggga tcgaaaacac cctcgagtcg ggaagcctaa
    10501 atatcgaact atccaaaaat cggcactgct gccggaaata gcagcggcgc taagtcggga
    10561 gggtagcctc ggatgtcatt tgcggcttcg ggagccgtta agtctgttgc gcatgcgaga
    10621 catcggataa gcggctgcag gccatcgcat tgccgcagca gcgccgtctc gagcggcgtc
    10681 agagatgtgg ccggacatgc attgctggtc atgccggatg ctgtgatcca tgtgccacat
    10741 ggcaatagat ggcaatgggc ccgcgtccgc ggccttccgc cgatcaatca ggcaaagatg
    10801 ctcgctccga cgtcggcgcg gccgacacgg tcgcggaaca gggcaaagag ttcgcgaccg
    10861 acaccagcct gcgcggcgga cagatccgcc tgcgctggca cgcgcgcgtt ccattcatgg
    10921 cgctcgacga gcacgttcag ccgcccggcg accgcatcga gacggatcag atcgccgtcg
    10981 cggatcttgc cgatcgcacc gccctccacc gcttcgggcg tgacgtggat cgctgccgga
    11041 atcttgcctg aggcaccgga cagccggccg tcggtgacga gcgcgacctt caggccccgg
    11101 tcctgaagcg cgccgagcgg gggcatcagc ttgtgcaatt ccggcatgcc gttcgctttc
    11161 ggtccctgga agcgcaccac ggcaaccatg tcgctggtga gttcgccggc cgcaaaggcc
    11221 ttttgcagtt cctcctgcga gtggaagacc cgggccggcg cttcgatcac aaggcgctcg
    11281 cgcgcaaccg ccgacgtctt gatcacggcg cgcccgaggt ccccgtcgag caccgtgagg
    11341 ccgcccgttg cctggaacgg ctcggcgacg ccgcgcagca ccgacgcatc accgctcgcg
    11401 gcgggcccgt cgcgccaggc cagcgctccc gccgcatcga gcatcggctc ctgcacatag
    11461 gcggcaaggc tcttgccgca aacagtcgag acgttgccgt gcaacagccc ggcgccaacc
    11521 aattcacgca tcaggaaact catgccgccg gcggcatgaa agtgattcac gtccgcgctg
    11581 ccattgggat agatgcggct cagcagcggt gtcacctccg cgaggtcggc gaaatcgttc
    11641 cagttcagcg tgaggccggc cgccgcagcc atcgcaacca gatgcagggt gtgattggtg
    11701 gacccgccgg tggcatgcag cccgacgatg ccgttgacga aggcgcgttc gtccagcatc
    11761 cggccgagcg gcgtatagtc gttgccgagc gacgtcatgg cgagcgcccg ctcggtggcg
    11821 gcgcgggtga gcgcatcgcg cagcggcgtg ttcggattga cgaacgacgc ccccggcaga
    11881 tgcaggccca tgatctccat cagcatctga ttggtgttgg cggtgccgta aaaagtgcag
    11941 gtccccggcc cgtgataggc cttggcttcc gcctcgagca gcgcctcgcg gccgactttg
    12001 ccctctgcgt aggcctggcg gatttttgac ttggcgtcgt tggcgaggcc cgaggtcatc
    12061 gggcccgacg gaatgaagac cgccggcaaa tgcccgaaag agagcgcgcc gatcacgagg
    12121 cccggcacga tcttgtcgca gatgccgaga aacaccgcgg cgtcgaaggt ctgatgcgac
    12181 agcgccaccg ccgtcgacaa tgcgatcacg tcgcgcgaaa acagcgacaa ctccatgccc
    12241 gcttcgccct gggtgatgcc gtcgcacatg gccggcacgc cgcctgccac ctgggcgacg
    12301 gcaccggctt cgcgcgcgct ccggcggatc agttcgggaa aacgctcgta gggctgatgt
    12361 gccgacagca tgtcgttgta ggcggtgacg atcgcgagac tggggacacg gccttcccgc
    12421 atcatggtct tgtcgcgcgg cgcgcaggcc gcaaaggcat gcgcctggtt ggcgcaaccc
    12481 aatcgctgcc gatgcggcgt cgcggccgcc tcggcgatgc ggtcgagata gcgcgcgcgt
    12541 ttgtcactgc tgcgccgggc gatgcgtttc gtcacctcgg cgacgcgggc attgaccgtt
    12601 gcctcgatta tcatgcgaat ctcccgtaga cgtttccgaa cagctttcga aatgatcacg
    12661 caacaaaggg cgcacgagcg cgcatccagc acccgactga cggcgatgtc gtgccgtcag
    12721 atcaagggct gtcctccatc caggtgcggc cgtcgcgctc gatcagcgcc gtcgcggcgg
    12781 aaggacccca gctcccgacc acataggatc gcggaacttc attcattcgg ttccaggcct
    12841 cgcggatcgg gtcgatccag cgccacgccg cctcgacttc atcgcgacgc atgaacaagg
    12901 tcgcattacc gcggaccacg tccatcagca gtcgctcata ggcatccggc accgagacgt
    12961 cgaacgcctt tgcaaagctc atgtcgaggg gaacatgttg caggcgcatg ccgccgggcc
    13021 ccggttcctt gatcatcagc cagagcctga tcccctcgtc cggctgcagg cggatcacca
    13081 ggcgcgtttg tgaaatccgg ccggtctcct tgtcgaaaat cgaatgtggc accttccgga
    13141 aggtaacgat gatttcggaa cagcgctgct gcagccgctt cccggtccgc agatagaaag
    13201 gaacgccggt ccagcgccaa ttggcgatgg cggccttgat cgcgacgaag gtctcggtcc
    13261 tgccttccgc atggccgatc tcatccgcgt agcccggaac cgcgacgccg ttgctcgccc
    13321 ccgcgcgata ttggccgagc acggtcagcg ccgctgcatt gctgtcgtcg atgcgcgcga
    13381 gcgattttag caccttcagc ttctcgtcgc gcaccgcatc ggcctcgagc gaattcggcg
    13441 gctccatggc gaccaggcac aacagctgga gcaggtgatt ttgcaccatg tcgcgcaacg
    13501 cgccggagct atcgtagtag gcgccgcggc cttcgacccc gatcgcttcg gcaaccgtta
    13561 tctgtacgtg atcgatgtgg gcggcgttcc agagcggctc gaacagcgcg ttggcgaagc
    13621 gcagcgccat caggttttgc accgtctcct tgccgagata atgatcgatc cggaagacgc
    13681 gttcctccgg aaacaccttg ccgatcgcct ggttcacgct gatcgcggat gcgagatcct
    13741 tgccgatcgg cttctcaatc accacccgcg cttcgttccc ggaaagaccg tggcggccga
    13801 gccttttgca gatcggaccg aacaactgcg gcgcagtggc gaggtaatag acctgaacgc
    13861 ggccggcaaa gggagccatc gcggctacaa gctcggccca cccgtcctcg ccctcggcgt
    13921 cgatcgcgaa gaacagcagg cgcgccagga agcggtcgag ctctggtccg ccggtttgcg
    13981 aggccgggat gtgttcgacg atggcgtcgc gcgcgagcgc gacgaattgg tcacggctca
    14041 tcggtcgccg cgccgcgccg agaatggtcg cggcctcggg cagctgaccg tcgaaatcgc
    14101 gatgaaacaa cgccggcaac agcttgcggc gcgcgagatc gccggtcgcg ccgaacactg
    14161 tgaggacgaa cggagccacc gggatcacgc ggctggtcat gcaagcccct ttctcgagtc
    14221 gatcacggaa ttttcgctga gccggccaac cgcggcgagg ttcgccgcga cgtccgcctc
    14281 ggtcggcaag tcggcgccgt tgcgcgagac ggtgacgctg gcggcggcca tggcgtagcc
    14341 gagcaggtcg cggatcgccg gcagatcgag cgcggcaatc gcatctggat caagcttgtc
    14401 ggtcttcgca agctgcgcca gcaaagctgc gtggaaggta tctccggcgc cgaccgtatc
    14461 gcgcaccacg acagaatggg cgggcaccga aacatgaccg gcagcgcaga acgccgtcgc
    14521 gcctttcgct ccctcggtca ccaccaccag gcgcgcgccg caattgagcc agtagacggc
    14581 tgcttccacg accgacacac ggccgccccc ggcggcgcgc acatcctcgt cgctggcctt
    14641 gatgatggtc gcggtccggt agaagcgctc ggccgccgtc gcccagcgcg ccatgtcccc
    14701 gaccacagtc ggccgcagat tgggatcgac gctgatcaca caccgcccgc attcgcgttc
    14761 ggcgagcgcg gcgaaagccg agccgaccgg ctcgaccgcc atcgtatagg agccgaaggt
    14821 cagcgcccgc acatccagcg gcagcgaggc cggcagatcg gcgagcccga gcgaacggtc
    14881 cgccgcgccc tcgccgtgaa aagcgtagtg cggctggcca ttgtcgtcag tcgcaaccat
    14941 tgagatcgtc gacagccgat cggtacgaac cagaaagcga tcgtcgatgc cctcgtgaac
    15001 gagaatgccg gccaagagcg cgccgaagtg gtcgcgcgag atgccgccga ggaaaccagc
    15061 tttcacaccg agccgcgcga ggccaatcgc gacgttgaag ggcgagcctc cggcaacggc
    15121 acgcgccggc atctccgagc cttcggccgg gccgacaaac aaatcgatca gcgcttcacc
    15181 acagaccagg atcatgccgc agctttctcg caggccgcgg ccacgcgaat aggttcagca
    15241 acgaggatgg ttggcctgcg catcggtcaa tccttgaaca gcacgtcgtg atctggggcg
    15301 aaggccgcga gcaagggcaa cgccgcaccg ccgatcgcgc gcgcatcgcg cccgatcgaa
    15361 ccttccacca ccactgcgtc ggtgaggccc tgctggtcga tgcgggcgcg ctgcgcacgg
    15421 gtctgcgcga caaggcgctc gcgcacgttg gcggggaacg cgccgtcgat cacgatcgcc
    15481 tcgaaatcga tcaccgccag cagcgaggtg atcgcctgcg ccagcgcctc ggcggcctcc
    15541 gagatccaga cgtcgagcgg ctcggcgaag ttcgaccagt cgtccggcga cagccagatg
    15601 tcggtgccgc gccggccggc ggcgatcaac cgacgctcca gccgatagat cgaggcggtg
    15661 cggatcagtt gctgcaggct cgcatcacca ttcggctcgc gacgcatgat cggcatcgac
    15721 ccgagcgcgc cggcattgcc gctgcgaccg ggaaacagtc tgccgttaag gacgatgccg
    15781 ccgccgatga aggtcccgac atagaagtag gcgaagtcgc gataggtcgc gccggcaccg
    15841 aaggtgagct cggcggcgca agccgacgtc gcgtcgttgc agaggcggac cggccaacgc
    15901 gcgaaacgac gcgacagctc ggccgcgaca tcgaaatgcc gccattgccc gagcgcgccc
    15961 ggcggcgtct cgagatcagc ctcccagttc cagagctcga acggcatcgc gacgccgagg
    16021 ccgctgatgc ggccgtgctg cgcgacgggc aattcagcca gcaggtcggg catctcccgc
    16081 tcggcgaagg cgagaatctt cgcgggtgcg ggatagggat agatcatgcg gcgccgcgcg
    16141 cggatgccgg cggtgaaatc catcaacacc aggtcggagc tgcgccggcc aatcatcagg
    16201 ccgagcccga aggcgccgtc cggattgagc gtatacggta ccgatggctg gccgatgcgg
    16261 ccgcgctgcg gctcgccggc aaccaacagt ccgtccgcct ccaggcggtt gatgatgacc
    16321 gtggtggtct gggtcgaaag gccggtcaga cgcgcgatct cgaccttggc gaggctgcgc
    16381 tggcggcgga tcagggacag gatcaggcgc tcgttataga gtccgacgcc gctctggctg
    16441 gtgccgcggc tcccagccga cagggcggtc atgggctggc tggcgcccgc aaacggcgct
    16501 ccttcatcca tcctcggctc cccaaaaaat ggcggctttt cctgcattgc aacggtcctg
    16561 ggcctaggtc cagccttcgc tcaagcttcg cttcggtccg gcaaggcgga cgccgccagc
    16621 ctcacaatgg gcggggttgt ggttgcctgt caataattaa atcggaatga tttatttatt
    16681 gacaagcggt ggccggcgat gcctcatagg gaaactgaag ggcaacaagg tatcgcgcag
    16741 tcgaccccgc gcagcctcaa tgtcagcccg atgggaggga aaaataatgc gtcagccact
    16801 cgtattcttt gccgcgctcg ggatcgccct gcgtgtcacc gccgccaacg ccgccgacaa
    16861 gccgatcgtc ggcctcatca ccaagaccaa taccaatccc ttcttcgtca agatgaaggc
    16921 cggcgccgaa gacgcggcga ccgccaatgg catcgagttg cgctcgttcg ccggcaaggt
    16981 cgacggcgac aatgatggcc aggtcgccgc cgtcgaaaat ctgatcgcca ccggcgcgaa
    17041 gggcatcctg atcacgccga acgactcccg tgccatcgtg ccggccatcg acaaggcgcg
    17101 cgccgccggc attctggtga ttgcgctcga cacgccgctc gacccgccga cggcggcgga
    17161 cgcgaccttc gccaccgaca atttcctggc cggcgaattg atcggtcagt gggcggcgaa
    17221 gacgctgggg ccgaaggcgg cctcggccaa gatcgcactg ctcgacctca acgtgaacca
    17281 gatatctgtc gacgttgcgc gggaccaggg tttcctgaaa ggcttcggca tcgatctcgg
    17341 caacccggca aagatcggcg acgagaagga tgcccgcatc gtcggacatg acgtcaccct
    17401 cggcagcgag gaaggcggcc gcaaggcgat ggaaaacctg ctgcagaagg atccgggaat
    17461 ttcagtggtc tataccatca acgaaccggc ggctgcgggc gcttatgagg cgctgcgctc
    17521 ggtcggccac gacaaggacg tgctgatcgt ctcggtcgac ggcggctgcc cgggcgtgca
    17581 taacgtcaag gacggcgtga ttggcgcgac ctcgatgcag tttccgctac tgatggcggc
    17641 aaagggcgtc gaggccgtga agaagttcgc cgagaccggc accaagccgc agccctcgcc
    17701 cgggcacgat ttcttcaata ccggcgtcga gctcgtgacc gacaagcccg ccgcgggcct
    17761 aacctcgatc gacagcgccg ccgccttgaa gaagtgctgg ggttaggcga agctcgcgga
    17821 tcggttaagg acgggcgccg cgcgacgtcg cgacgcctgg caaacaaagc gatgcccctg
    17881 ccgaccgccg gcggccaatc ctgtggttcg aggaaggcgc gatgaccgat atcggcaaca
    17941 gccaccccgc catccaggcc cttgaggtcc aattggacag gaaggagctg gacaagaagg
    18001 acgaccgcgt ggtcgaattc gaggtccgcg ccaagtcccc gctgcggaag ctgcagcatt
    18061 ttctccacgc caatcctact gcggtgccgg cgatcgtgct gttcctcggc attgcagctt
    18121 tcggtctcat cgtcggctcg cgcttcctct cctccttcaa tttgtcgctc atcatccagc
    18181 aggtgacgat catcggcgtg atcggcgtcg cccagacgct gatcgtgatc accgccggca
    18241 tcgatctgtc cgtaggcgcc atcatggtgc tgtgctcggt gatcatgggc aagcttgcgg
    18301 tgacggccgg cctacccgct cagcttgcgc tcctgatcgg aatcttagcc ggcggaggct
    18361 gcggcgcggt caacggcatc ctggtgacgc ggttcaagct gccgcccttc atcgtgacgc
    18421 tgggcacctg gtcgatcttt ttcgcgctca atctctggta ctccgcctcg gagaccattc
    18481 gctcgcagga cgtggcgaaa accgcgccgt tcctgcaggc cctgggaacg cccgtcgaca
    18541 ttcttggtgc ccgcttcacc tacggctcgt tctttatgct ggccctggtc ggactgatct
    18601 ggttcgtgct caaccgcacc gctttcggcc gccatctcta cgcagttggc gacgatcccg
    18661 atgcggcgcg gctcgccggc atccgcaccg ggcgcgtcct gttcacggtc tatgtggtgg
    18721 cgggcgtgat ctgcgcactc ggcgcctggg ccctgatcgg acggatcggc tcgatcagtc
    18781 cccaggccgg ccagaccgcc aatctcgaca gcatcacggc agtcgtggtc ggcggcgcga
    18841 gcctgttcgg tggacgcggc tcgatcgtcg gcacgctggt cggcgccctg atcgtcggcg
    18901 ttttccgcaa cgggctcgcg ctctccggcg tcgacgtgct gtggcaggaa ttcactgtgg
    18961 gctggctgat catcgtcgcg gtcgcgctcg atcaatggat ccggaaggtt tcggcatgag
    19021 caacgacggt cagacgttca aatccacgtc agcgtcaacg ccgatcctgc aggcacgcgg
    19081 cctcgccaag cgttacggcc gggtcgtcgc gctcgacaac gccgacttcg atctgctgcc
    19141 gggcgagatt ctcgccgtga tcggcgacaa cggcgccggc aaatcctcgc tgatccgagc
    19201 cctctccggc gcgctgacgc tggatgcggg cgaaatcaga ctgaacaacc agccggtgac
    19261 gttccgctcg ccgctcgatg cgcgggccgc cagcatcgag accgtgtatc agacgcttgc
    19321 gctgtcgccg agcctatcca ttgccgacaa catgttcctc ggacgcgaac tgcgccgcgt
    19381 cggcccgctc ggctccgtct gccggatgct ggatcgcaag gccatgcagc gcattgcacg
    19441 cgagaagctg accgagctcg gcctgctgac ggtgcagaac atcaatcagc gcgtcgaaac
    19501 cctgtcgggc ggccagcggc agggcgtggc ggtggcgcgg gcagcggctt tcggctcgcg
    19561 cgtggtgatc atggacgagc cgaccgcagc gctcggcgtc aaggaatcgc gccgcgtgct
    19621 cgaactcatt ctcgacgtgc gccggcgcgg cgtgtcgatc gtgctgatca gccacaacat
    19681 gccgcacgtc ttcgaaatcg ccgaccggat tcatatccat cggctcgggc ggcggctgac
    19741 cgtgatcgac ccgaaatcct acaccatgtc ggacgcggtc tccctcatga ccggcgcacg
    19801 gttgccggtt gagatcgccg cgtgagacta ccggcgctga attgagttgg atccggtcta
    19861 tcgggcgata gcctgaccag cagggcgtta cacgggacgc cgcgcgcggc gttcgtcacg
    19921 agcagaccgt tggtctgcgc ggcggtcggg atttcccgat cacccgagca aggcagtaca
    19981 ggtctgggct cacatatcgt ccacaccatc gtcacaaatt gtctgggtgg ccgacttgat
    20041 ctcgatagcg agcccggcca agggaaatcc agcttatcct gccgcgggta gcaccggccg
    20101 gacttatcac tgaatagcac gccgttcgac tcagtaggcg gctgcaagat tttcgtgttc
    20161 taggacgcct atattcagcg ggtattgaaa tgtctgctca gggtcaacct ggaatccggt
    20221 gatagacttc gaccagatcc gccctagctt caacagcggc gtaggcgaca aatgcacggt
    20281 ttggatttgt ggagcggaaa cgtgtgcatg gcgaaggggg acgaagaggt tcggatcgat
    20341 gacgctcctg tcatttccat ccgcgcagac gaggccctcc ggacgcttgc tcgcctgatc
    20401 gatcgtcaga tggcacgcga gcagttcgaa cctaaacaag ctatagtgca caagcgccgc
    20461 catctcgtgc aaacaaacaa gtcgccaact cgcgaacagc accataggtc cattcccaca
    20521 gtgtgacttc gccgacgttg tacctatcct tgcgaaagct atcaaagtac cggtcgattc
    20581 catgaccacg acgacgatct tgaggttccg ccgcaagctc ggcgcgtttc agcaacgcgc
    20641 ccgctgcgag cctattgaga tcacacgcca tggtcggcgc gcgttcgtat tgatgtcggc
    20701 tgagcattgc gactggatgc agggctgcca gccggcggac gtatcgtgct tctgatgcgg
    20761 ccgccgttgt gatcgacgcg gtgaagcggg ccacaatgct cccggcacat gagccactca
    20821 acgaactgtt gaagtgaagt gcttccgctg cgcggagctt tggccatcgg aatcagaatc
    20881 gtagcgcttc ccgacccacg ttcgcgccac tgcgacatga tgtgtgtggt tgcggggtga
    20941 tttgagccta tgagctaagt cattgatgtt gttagggtcg attacgacac aaaaagaacc
    21001 ccagcaatcc attgagatta ctggggtttt ttatcaactt ggttgcggta gagtcgagat
    21061 gtggcgcggt ggctttaggt tcgaacatat ctgttcccgc ctctttcgtc tagcggtgcc
    21121 tcactggctc gaccatggcc ccgtttccac atcccgctca tcggtagggt cgagggctgg
    21181 cgcgccggtg tggctcgggg gtcccgaccc acaccacgtt tccagccccc tccacgtcgc
    21241 acgcaacgtg cggatttccc gcattgcgcg cccccatttg cttcacgcca aagctgatgg
    21301 gacctatcct gttgggacga ctttcggccc gtcgcgtcgc actcgatagg cgttgaacag
    21361 ccctagagtg tcgtacagcc atcgcctatt ccatcgttcc cagtcgaagc cctttcgttt
    21421 ctgagcatgc gccagatggc gcctgatctt cttttccatc caatccttga tgaagctgaa
    21481 gcactcgctg gagtgcccaa ctgcgaagta gttcacccat ccccgaagca ccggattgat
    21541 caggcttatc acccgctcga tcggttgcga ccggtgtcgg cggaacactt ccttgagctc
    21601 ccgcaacagc gccgtccgct ttttgagctt gggcgtatag tgcggccgcc atgccccgct
    21661 gtgaacgctg cggaggtaac ggaagtcgaa gcccaggaac ccgaagcttt cgcctcggcc
    21721 aaggtctacg gtccggctct tctcatcatt gatttcgacc tggagtttgt cgaactcctc
    21781 ccggaggcgt ttttccaccg ctccgatcag ccagtcatgt cgcttgtagg catcgaccaa
    21841 gatcaccaga tcgtcagcaa atctcgcata ttcgatatag gtgtacttgc cttggtgagt
    21901 ggtctccttc gctcgctcca gcatcctgtc cacctcattg aggtatagat tgctgagcaa
    21961 cggggaaatt acaccacctt gtggaacgcc cctcttcccg gatgctttca gcatcacctt
    22021 cagaagatgc atgatgtccg cgtcattaat ccgcttcgcc accttttcca ataaccggtc
    22081 atgccggaca ttatcaaaat agctccgcag gtcgaagtcc aggacgcgcg tcttccgttg
    22141 gacgatggct tcggccaccc gctttatcgc gtcatgcgct gtccgcttgg gacggtatcc
    22201 aaacgagccc ggttggaagt ccgcctcgaa aacaggctcc actatgagct tgagcgcccc
    22261 ttgaaccacc cggtcccgaa tggccggaat cgaaaggacg cggactttgc ccccatcctt
    22321 cggtatttcc tgccttcggg ctgggagcgg cttgtaggtg cgcccgatca gttcgtcccg
    22381 tagctgttcg agcagagctt ccgcgccctg cgcctcgatg gcctcgaacg tcaccccgtc
    22441 aactccaggg gctccgtcgt tctttttggc cattttgtac gcttcgcgca gcgtctccat
    22501 cttgcagacg tggacgtaaa gaccccagaa acgccaggac ggttcagcct tcgccttgac
    22561 gtatatcctc cgcctcaggt cctgcaaatc gatggacgcc tttgtcatct cgtccttgcc
    22621 tttccttgtg ttggaggcgt tacaaatggc agggcccctt cgctccgcgg acgttgctcc
    22681 gcttcatcgc taatacggac ccatccgcca ccctctcgtc ttcgaccgat ttcccgggtt
    22741 cgccggttat acggtctacc ttgctccgac gatttcgcgc cgggacgagg agggcttctc
    22801 cagttgctta gcatgtcctt gtcaccgtgc tgtcgcttcc accccgccga ggtgaacatc
    22861 cgtatcggtc agtcttcggc tgctcatgcg gccttcgccc tccggttgaa ggctcggccc
    22921 tcggagatac tcattttcga ggccacaatg cgttcactgt tgttacggcc cggtgactcg
    22981 t
//
LOCUS       DASWGM010000505         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2134075,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000505 DASWGM010000000
VERSION     DASWGM010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134075"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="VGA15_14515"
     CDS             complement(<1..2003)
                     /locus_tag="VGA15_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HEX9588945.1"
                     /translation="MLLATSALASAALPTAVAHADDAVWNPAGDTNFNNNGNWTDGAG
                     GAGVVPTGTATFNVSDTRAITFNDLTTTLGGMTVTSGAGDYKFETNGKSITFNGAGLS
                     VGSGASLTINNRDGGTVIFSDSSNAGSNNSNADHAVKETVIDNFGGKLLFAVNASAET
                     AVINNFSSGTDVFKFDNESTAAAARIQNHGDLHFVGNSTAGNAFIVSFIQSSGSESHI
                     FIEENANGGTARIQLDNGSLDISKLVTVGTTVGTTVGSIEGSGNVFLGSKNLKVGGNN
                     LQFAKEFSGVIQDGGDGAGIGGSLTKEGTGALTLTGINTYTGKTDVNGGFLVVDGLGS
                     IASSSGVTVNAGGTLAGTGTVASTIVDGGTLAPGHSDGSFGKLKVQGDLLFTQGAGTT
                     STYLVQVSPANATSTSVTGSARFNGATVQANFAPGSYVPKEYNILHAGDPLSDKFGSL
                     VTTTNLSPMFKATLSYDANNVFLDLSANSGAISGLNTNQRNVSNAIFNSLNLMNGLPF
                     AFMTATQAPASLTQISGETAVGTQQATFDAMNQFMGVMLDPTVGALSGGRTKDAFAAM
                     PGKAAPLAAEAFNARWGVWAAGFGGSQSTNGDAVLGSNKTTSNVFGSAAGAEYRFSPN
                     TVAGFALAGGGTNFRVANGGSGNSDLFQAGAYLRHTQGAAFIAA"
BASE COUNT          400 a          694 c          806 g          495 t
ORIGIN      
        1 gcggcgatga aggctgctcc ttgcgtatgg cggagatagg cgccggcctg gaacaggtcg
       61 gagttgccgc tgccgccatt ggcgacacgg aagttggtgc cgccgccggc cagcgcgaag
      121 ccggcgaccg tgttgggcga gaaccggtac tcggcgcccg ccgctgagcc gaacacgttg
      181 ctggtggtct tgttggagcc gagcacggcg tcgccattgg tgctctgcga tccgccaaag
      241 cccgcggccc agacgcccca gcgtgcattg aaagcttctg ccgccagcgg cgccgccttg
      301 cccggcatcg ccgcaaaggc gtccttggtg cgtcccccgc tgagcgcgcc gacggttggg
      361 tcgagcatca cgcccatgaa ctggttcatg gcgtcgaagg tcgcctgctg ggtgccgacc
      421 gcggtctcgc cggagatttg cgtcagactc gccggcgcct gcgtcgcggt catgaacgcg
      481 aacgggaggc cgttcatgag gtttaaggag ttaaagatcg cattgctgac attgcgctgg
      541 ttggtgttga ggcccgaaat agcgccagag ttcgcgctca gatcgaggaa gacgttgttg
      601 gcgtcgtagc taagggtggc cttgaacatc ggcgacaggt tggtggtggt gaccagggag
      661 ccgaacttat cgctcagggg gtcgcccgca tggagaatgt tgtattcctt cgggacataa
      721 ctccccggcg cgaagttggc ctgcacggtg gcgccgttga acctcgccga tccagtgacg
      781 ctggtgctgg tggcgttcgc gggcgaaacc tgcacgagat aggtcgaggt cgtccccgcc
      841 ccctgggtga agaggagatc cccctgaact ttcagcttgc cgaagctgcc gtcgctatgc
      901 ccgggcgcca gtgtgccgcc atcgacgatc gtgctggcga cggtgccggt tccggcgagg
      961 gtgccgccgg cgttaacggt cacgccgctc gatgacgcga tcgagcccaa gccgtcgacg
     1021 accaggaagc cgccattgac gtcggtctta ccggtatagg tgttgatgcc ggtgagggtg
     1081 agggcgcccg tgccttcctt ggtgagcgaa ccgccgatgc cggcgccatc gccaccgtcc
     1141 tgaatcacgc cggagaactc cttggcgaac tgcagattgt tgccgccaac cttaaggttc
     1201 ttgctgccga ggaagacgtt gccgctgcct tcgatcgaac cgaccgtcgt tcccactgtc
     1261 gttcccactg tcacgagttt ggaaatatcg agagaaccat tgtcgagctg gatgcgcgcg
     1321 gtgccgccgt ttgcattctc ctcgataaag atgtggctct cggagccact tgattggatg
     1381 aaggatacga tgaaggcgtt gccggcggta ctgttgccga cgaaatgaag gtcgccgtga
     1441 ttttggattc tcgcggcggc ggcggtgctc tcgttgtcaa atttaaaaac gtctgtacca
     1501 ctcgaaaagt tgttgatgac agcagtttcc gccgaagcat taacggcgaa cagcaatttg
     1561 ccaccgaagt tgtcgatcac cgtctccttc accgcatggt ccgcattgct gttgttgctg
     1621 cccgcgttgc tgctgtcgct gaagattaca gtaccgccat cgcggttgtt gatcgtgagg
     1681 ctggcaccgc ttccgacgga gaggccggcg ccgttgaagg tgatggattt gccgttcgtc
     1741 tcgaacttat agtctccggc gcccgacgtc accgtcatgc cgccgagagt ggtggtgaga
     1801 tcgttgaatg tgatggcccg ggtgtcgctg acattgaacg tcgcggtgcc agtggggacc
     1861 acgccggccc cgccggcccc gtcggtccag ttaccgttat tgttgaaatt ggtgtcgcct
     1921 gcgggattcc ataccgcgtc gtccgcatgg gcaaccgcgg tgggcagcgc cgcggacgcc
     1981 agcgccgagg tcgcgagcag catgattcgc aggcgtgacg cccggcgcgg cgcagcgcgg
     2041 cggctgtcgg cgtgatggga tgaatgtgaa gcttgcggtc gcatggtggc cccttcaagg
     2101 tgtttcgtca cctcgttacc gttgcagggc gcggcgcgct tccggcgaac gggggaaacc
     2161 gtcggtttga tcaagagtgt tgatttttcg cttcacccga ttgggggaag catggagcgt
     2221 cgacagccgc cgcgtgtcgc ggatcgagga cggtctagac gagcgattcc atgaaaacga
     2281 tcctatggga tttgacccaa cgtttgtcat tccgggatgc gttcctgccg gcgatagccg
     2341 gtcggagcgc aggcccggaa tccatagccc ctgcggtggt tatggattcc gggct
//
LOCUS       DASWGM010000506         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2415057,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000506 DASWGM010000000
VERSION     DASWGM010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2415057"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGA15_14520"
     CDS             <1..234
                     /locus_tag="VGA15_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588946.1"
                     /translation="GLVLALITMVTSEMIARQSGAGNILFNALDMGQYDTVYAMIIII
                     GALGIGLDAAFEKLRGRLVKWSEPGFDVPLSFA"
     gene            231..998
                     /locus_tag="VGA15_14525"
     CDS             231..998
                     /locus_tag="VGA15_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9588947.1"
                     /translation="MNARAVTTDVLLGIVPIALLIALWQAIASFGYAPASLLPPPGLV
                     FMRLAQQLLTGTFQHEIGATLFRLFVGFSIAVILGVAIGLAAATSPAVNSVVRPIVRV
                     LAPLPKVALYPALLLLLGFGHESKITLVVADALFPILLSTYYGASMVEQKLIWSAMAA
                     GTPRRQILFKVVLPAAMPSILTGCRIGLVISCIVVFLAEMITSTDGLGHLLVTAARTF
                     QAVDMFVPLITVSLLGLILNGLLQALRAFLLRGFPEV"
     gene            1025..2071
                     /locus_tag="VGA15_14530"
     CDS             1025..2071
                     /locus_tag="VGA15_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M29"
                     /protein_id="HEX9588948.1"
                     /translation="MLADRIEAKWIDAFCEIFERCAVKPGDTAAILSETQSRALNVHL
                     AELALLRMGAKPFHIIVPTPRNRQAVPIRSTGASIAIQRLAPVVTALQQAGFVVDCTI
                     EGLMHAAETPEILKAGARILNISNEHPEALERMVPDTALEKRVRAAVKMLRGTKRMRV
                     TSKAGTELDVDMAGASTVGVWGWTDKPGTLAHWPGGIVVSFPKSGTVNGTLVMAAGDI
                     NLTFKRYLTSPIRMTLKDDYVTDLEGEDADAMMMRAYLAAWGDREAYAVSHVGFGMNP
                     GARYEALAMYDQRDTNGTELRAVAGNFLFSTGANEFAGRYTAGHFDLPMMGTTIELDD
                     VAVVREGVLQDVFG"
     gene            complement(2220..>2411)
                     /locus_tag="VGA15_14535"
     CDS             complement(2220..>2411)
                     /locus_tag="VGA15_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX9588949.1"
                     /translation="DRYRAPEVISRNMHDGRIGMRTGAGFLDYSGLDVDAYREQRLAA
                     MVDMLRHFNLARPPVLDRD"
BASE COUNT          424 a          704 c          774 g          509 t
ORIGIN      
        1 gggctggtgc tggcgttgat caccatggtc accagtgaga tgatcgcgcg gcagtctggt
       61 gccgggaata tcctgttcaa tgcgctggat atgggacaat acgataccgt ctacgcgatg
      121 atcatcatca tcggcgcgtt ggggataggc ctcgacgccg cattcgaaaa gctgcgcggt
      181 aggctggtaa aatggtccga gcctggcttc gatgttccgt tgagctttgc atgaacgcac
      241 gcgccgttac gacagacgtt ttgctgggga tcgtgccgat cgcgctattg atcgcattgt
      301 ggcaggcgat tgcgtcgttc gggtatgcgc cggcgtcgct gctgccgccg cccggactcg
      361 tgttcatgcg cctcgcgcag cagctcctta ccggaacgtt tcaacatgaa atcggcgcca
      421 cgctattccg gttgttcgtg gggttctcga tcgcggtgat tcttggcgtc gccatcgggc
      481 ttgctgcggc aaccagtccg gcggtcaatt ccgtggtgcg tcccatcgtg cgggtgctgg
      541 cgccgctgcc caaggtcgcg ctatatccgg cgctgctgtt gttgcttggt ttcggtcacg
      601 aatcgaagat cacgctggtt gtcgcggatg cgttgtttcc gattctgctt tcgacctatt
      661 acggcgcgtc gatggtggaa cagaaattga tctggtcggc gatggcggcg ggcacgccgc
      721 gccgtcaaat cctgttcaag gtggtgctgc cggctgcgat gccgtcgatc ctcaccggct
      781 gccgcattgg ccttgtgatt tcatgcattg tggtgttcct ggccgagatg attacgtcga
      841 ccgacggcct cggccatctc ctggtgacgg cggcgcggac ctttcaggcg gtcgacatgt
      901 tcgttccgct gatcacggtc tcgctgctgg gattgattct caacggcctg ttgcaggcgc
      961 ttcgcgcctt tttgctgcgt gggtttccgg aagtctgatg caacgatcga agtggagata
     1021 aagcatgctg gcggatcgca tcgaagcgaa gtggatcgac gcgttctgcg agatatttga
     1081 gcgttgcgcg gtcaaacccg gcgataccgc tgcgatactt tcggagacgc agtcacgcgc
     1141 gctcaacgtt caccttgccg aactggcgct gttgcggatg ggcgcaaagc cgtttcacat
     1201 catcgtaccg acgccgcgca accggcaagc cgtgccgatc cgctcaaccg gcgccagcat
     1261 agcgatccag cggcttgcgc ctgtggtcac agcgcttcag caggcaggct tcgtcgtcga
     1321 ttgcaccatc gaaggcctga tgcacgcggc ggaaacaccg gaaatactca aagcgggggc
     1381 gcggattctc aatatttcca atgaacatcc cgaagcgctc gagcgcatgg tgcccgatac
     1441 ggcattggaa aagcgcgtgc gggcggcggt gaaaatgctg cgtggtacca agcggatgcg
     1501 cgtgacttcc aaagcgggga ccgaactcga cgtcgatatg gccggcgcct cgacggttgg
     1561 ggtctggggc tggaccgaca aacccggcac gctggcgcac tggcccggcg gcatcgtcgt
     1621 cagctttccc aagagcggca ccgtgaacgg tacgctcgtg atggcggctg gcgacatcaa
     1681 tctcaccttc aagcgctatc tgacgtcgcc gatccggatg acgctgaagg acgattatgt
     1741 cacggatctt gaaggcgagg acgccgacgc gatgatgatg cgagcctatc tcgcggcttg
     1801 gggcgaccgc gaagcctatg cggtatccca tgtcggcttc ggcatgaatc ccggcgcacg
     1861 ctatgaggcc ctggcgatgt acgaccagcg cgataccaac ggcaccgagc tgcgcgcggt
     1921 ggccggcaat ttcctgtttt ccaccggcgc caacgaattc gccgggcgct acaccgccgg
     1981 gcatttcgat ttgcccatga tgggcacgac gatcgagctc gacgacgttg ctgtggttcg
     2041 cgaaggcgtg ctgcaggatg tattcgggta gccgctgcag ccaaatcgca ccgcagccac
     2101 cgccgtcatc ctgaggagcg cgctcttttt tgcgcgcgtc tcgaaggatg ggccgtatgc
     2161 accggtcttg ccatccttcg aggctcgcaa gtgctcgcac ctcaggatga cggcgaacat
     2221 taatcccgat cgagcaccgg cggtctggcc agattaaaat gccggagcat atcgaccatt
     2281 gccgcgaggc gctgttcgcg ataggcgtcg acatcgaggc cggaataatc gaggaaaccg
     2341 gcgccggtcc gcatgccgat gcggccgtcg tgcatattgc gtgagatcac ctcgggcgcg
     2401 cgatagcggt c
//
LOCUS       DASWGM010000507         9408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2553136,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000507 DASWGM010000000
VERSION     DASWGM010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2553136"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VGA15_14540"
     CDS             <1..403
                     /locus_tag="VGA15_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX9588950.1"
                     /translation="EEVHESCAKCTDDRKNAPLLGISFIRNMKRDGLKYEDGNVLDPR
                     DGKIYKAKMSVSPDGQSLTMRGYWGISLLGKDETWFRLPDTEMASLDPAIVAKYLPAQ
                     AAAQAAAIKPPVATVKPTVAAKKSASPQLPK"
     gene            510..887
                     /locus_tag="VGA15_14545"
     CDS             510..887
                     /locus_tag="VGA15_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX9588951.1"
                     /translation="MNDRAPDLGELEREVMQLVWSAGETTADAIRERLARKPKESTVR
                     TVLRRLEEKGYVTHTVDGRTYVFRAAEARPQVAARAVKAIVDWLCNGSVEEVLVGMAD
                     ARMLDSRTLQRLAKRIEQAKGGK"
     gene            887..2839
                     /locus_tag="VGA15_14550"
     CDS             887..2839
                     /locus_tag="VGA15_14550"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HEX9588952.1"
                     /translation="MLEVVVRAAICTLLLAFAVQLCLRTLRVRHPKLLLSAWTAVLVA
                     SVAMPALQRAIPSAAPTLLSRSFSRAGIVNSGAPAPLLPLSMISSSLTSLATSPSGAT
                     TARASEPQRTFAWRKWLTAAYVLVASLFLLRLLLGLILSWRMLRATHPVRENWAAGKR
                     LRTSTWICAPVTVGSHVLLPAECVNWDARRRRAVLAHEEAHLARGDFYVQLLSQVNRS
                     VFWFSPQAWWLHRRLTALAELASDDAAIEALGDRPGYAAILLDVARLPRNPSLSVAMA
                     RPATVCQRIERILAEETTPAPVTRLLQTVIAAAVAPLAMLTAVLFAEAAPLEASREQV
                     APHTRITIDPKLLDAYAGFYLNAATDSLMIVTREDDHLLTRRAGNQPVPEYPYTDHDF
                     FLTVVPQQNSFVTDASGAVLRVVHHQWGRDETLERIGAEAAQRHEEARLKRVAEERTP
                     RTAISIDPQLLDNYVGAYQLKPQLVFTVTREGDKLLAKLTGQPTYEVHPYTERDFFYT
                     IVAAQLSFVPGADGKASAVVLHQNGKDQTAPRVDLSVAQALDVKLAEQSAPRTAVKID
                     PSLLDGYVGRYSNAELEITATREGEQLFVQVTGYGRYAIFPYTEHDFFATIQPTQYTF
                     ATDNTGKATQMVRHRRGKDEVFIRED"
     gene            3331..5262
                     /locus_tag="VGA15_14555"
     CDS             3331..5262
                     /locus_tag="VGA15_14555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX9588953.1"
                     /translation="MSARPLIPRRKLFDNPTFFGAKLSPDGRLISWLAPVDGVLNVWV
                     SPVDSIAAGVPVTRTKGRPINWQDWSPDGRHIMFLNDENGDENLHLFVVDPRNCELRD
                     LTPFANIRAIPTYWSRIAPGKIAINLNDRDRRWHDVFLLDLATGQRSLIWENRQGFEF
                     VGLDWQLKPRHARSHGSDGGARLWHLDGEQVTHWRDVPFDASWTTRLWSFDASGKHLH
                     MLSCIEQDKSALVQIDWSSGEQKVVFESQRADVTDAIFNARTFEPEAVCIDPARQEWS
                     ALTPAVAAELALIKARLPGHAFHVQSQTDDDRRWIVMSYTAEQAATYHLLDRDKQTLT
                     ELFTARPELKPYRLAPMHAVEGRSRDGLTLVSYLTLPADIEGDRPAAPLPMVLIVHGG
                     PWARDIFGFRGDHQWLADRGYAVLSVNYRGSTGFGKAFVAASEKQHARKMHDDLIDMV
                     DWAVGEEIAQKDKIAIFGVSYGGLASFIAATFTPEVFCCAVPVVGISNLQTLLESMPP
                     YWAGFAEYMYRSYGDPRTEAGRKLLAERSPINRVENISKPMLIFHGVNDVRCKVAESD
                     TIVAAMRARNIPVTYVVYPDEGHGFQKPPNRLSYIVMAEAFFARHLGGAFEPVGSDLE
                     GSSHEIRAGVDILTDLGVT"
     gene            complement(5338..5985)
                     /locus_tag="VGA15_14560"
     CDS             complement(5338..5985)
                     /locus_tag="VGA15_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588954.1"
                     /translation="MLSYLNAETGFIRAFGNRSLLLIFLCALSRSGDHPGKASALGVS
                     LDLNPGLIVVFGPLLALLFLISLKIESDTLLVAREFVVGKMSHLDPGATKPSRWVFGL
                     FAVPCATATFMIVQFILNVVPAKPGCTGYVWTRKFTDFSVQGGTPSTYCIHNLTDGMP
                     WIYPPVQTYGYVVCLAACFYLTYLIARNWVRVRPHEVEVSKPEASRREASEPAVA"
     gene            complement(6078..7955)
                     /locus_tag="VGA15_14565"
     CDS             complement(6078..7955)
                     /locus_tag="VGA15_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588955.1"
                     /translation="MQISWEVRNRFRLFREERDFLLHTDSARGRSILGSEQALELQSD
                     GRGWARNTVNRLCIDLTGRVSEPCTRDNVKESYLTPSDHPVTLRLTGTIPVGATCAWS
                     IDDGEGPPRQSTLDCAEPLGFRARYGRPTMASVDVSSGPDAPQRVATEIMVRDIFIAG
                     LGDSIASGEGNPDRPIALSDEGFCFRSYLGTAVAQYYRPSRAGYKGGRACEAPDSLQV
                     WQRQSALWFNSACHRSLYSYQTRTALQLAVQYPHIAVTYLPLACTGATIPDGLFGGQR
                     ARECPPTKSTGCGATVNAQLAELRDAVAAAKRRQPDRNLDLVLLSIGANDIGFSGLVA
                     DVIVDTPTERVLFRRTGVMGSVEDSRAALAQDLPQSFGKLREALKALVGDMSHVVYVS
                     YANPTLADRAPCPGGRAGFDIHPSFNAQPQRLANVSGYVQNEFLPQLKALALCQSGIL
                     CRDPAGDRMTFVDGHQAAFADHGFCARGPNDPEFDRECFSAKGDSFDPDIVTSASQPM
                     LCGRSAGEYRAYLPRARWIRDANDSYFAAMTYPQGLPSSSQPADIHDATWGVLSAVYG
                     GAVHPSAEGHAAMADAAFPAAAAVLQLDAAVPEVTREPAAPIVTAPGARRPAGQPSGL
                     Y"
     gene            complement(8231..9292)
                     /locus_tag="VGA15_14570"
     CDS             complement(8231..9292)
                     /locus_tag="VGA15_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEX9588956.1"
                     /translation="MHRLLTTLGRGFKKRIGWKRLGIVASLLIIAFAITTLVRTLKGV
                     DTGVILTALTEIPPAHIGLAALCVLGAFCTLTFYDFFALRTIGKKNVPYRIAALSSFT
                     SYSIGHNIGATVFTGGAIRFRIYSDYGLSAIDVAKICFLSGLTFWLGNTFVLGIGMAL
                     HPWAASAMDQLPPAVNRLIALGGIAAIISYLVWLAMGDERRELGQNGWKVILPSAPLT
                     LLQILIGVVDLGFCALAMYLLMPAQPGIDFVSLAVVFILATLLGFASHAPGSIGVFDA
                     AMLVALPEFGREQLVATLVVFRILYFVIPFGIAISVMGTRELWLNVVLPWQERRKLHE
                     ACPPSSAVPQPVKCRPRKG"
BASE COUNT         1798 a         2924 c         3009 g         1677 t
ORIGIN      
        1 cgaagaggtc catgagagct gcgcgaaatg caccgacgac cgcaagaacg cgccgctgct
       61 cgggatttcc tttatccgga acatgaagcg cgacggcctg aaatatgagg acggcaacgt
      121 tctcgatccc cgcgacggca aaatttacaa agccaagatg agcgtcagcc cggacggcca
      181 gtcgctcacg atgcgcggct attggggaat ttcactgctc ggcaaggacg aaacctggtt
      241 ccggttgccg gatacggaga tggcctcgct cgatcccgcc atcgttgcga aatatttgcc
      301 ggcgcaggct gccgcccagg ccgcagcaat caagcccccg gtcgcgacgg tcaagccaac
      361 cgttgccgcg aagaagagcg cgtcgccaca gctgccgaag taatcggccg cccgccggcg
      421 gagccgcccg gtcaacgccc ccgggcttga cgcgaggtcg ccgtcacgca tatgctaccg
      481 atcgtagcat tacacgaggt ggcgcaccga tgaatgacag ggctcccgat cttggtgaac
      541 tcgaacgcga ggtcatgcag ttggtgtggt cagcgggaga aaccaccgcc gatgcaatcc
      601 gtgagcgctt ggcccgcaag ccgaaggaat cgaccgtacg caccgtgctg cggcgactgg
      661 aggaaaaggg atacgtcacc cacaccgtcg atggccgcac ctacgttttc agggctgcgg
      721 aggcacgacc gcaggtcgcg gcccgcgcgg tgaaggcaat cgtcgactgg ctctgcaacg
      781 gatcggtcga ggaggtgctg gtgggtatgg cggacgcgcg catgctcgac agccggacgt
      841 tgcaacgact tgcaaagcgc atcgagcagg caaaaggagg aaagtgatgt tggaggtcgt
      901 cgttcgcgcg gcgatatgca cgctgctgtt ggctttcgcg gttcaattgt gcctgcggac
      961 gcttcgcgtg cgccacccga aattgctgct ctccgcctgg accgcggtat tggtcgcgtc
     1021 cgtggcgatg ccggctctgc agcgggcgat cccatccgcc gctcccacat tgctctcccg
     1081 gtcattttcc cgggcaggca tcgtaaactc cggcgcaccg gcgccgctgt tgcctttgtc
     1141 gatgatctcg tcgtcgttaa cctcgctggc cacttccccc tcaggcgcga ccacggcgcg
     1201 cgcatcagaa ccgcaacgga cattcgcctg gcgaaagtgg ctgaccgcag cttatgtctt
     1261 ggtggcctct ctctttctgc ttcggctgtt gctgggcctc attttgtcct ggagaatgct
     1321 gcgtgccacg cacccggtgc gggaaaactg ggccgctggc aaaaggctgc ggacaagcac
     1381 ctggatttgc gcgcccgtca ctgtcggcag ccacgtgctg ctgccggcgg agtgcgtcaa
     1441 ctgggatgca cgcaggcgcc gggccgtcct cgcgcatgaa gaggcgcatc tcgcgcgtgg
     1501 cgacttctat gtccagcttc tgtcgcaggt gaaccggtca gtcttctggt tcagtcctca
     1561 ggcctggtgg ttgcaccggc gcttgacagc gctggccgaa ctggcgagcg acgatgccgc
     1621 gatcgaagcg ctgggcgatc gtccgggcta tgcggcgatc ctgctcgacg tcgcgcgcct
     1681 gccgcgcaat ccatccttaa gtgtggcgat ggcgcgcccg gccacggtct gtcagcgcat
     1741 cgagcgcatc ctggctgaag aaaccacgcc cgctccggtc accaggctgc tgcagaccgt
     1801 gatcgcggcc gcagttgcgc cgctggcgat gctgacggcg gtgttattcg ccgaagcggc
     1861 gccgctggag gcatccaggg aacaagttgc gccgcacacg cggatcacca tcgatcccaa
     1921 attgctggac gcctatgccg gcttctacct gaatgccgcc accgactcgc tgatgatcgt
     1981 gacccgcgag gatgatcatc tgctcacccg tcgggccggc aaccagccgg tgcccgaata
     2041 tccatatacc gatcacgact tttttcttac ggtcgtcccc cagcagaaca gcttcgtcac
     2101 cgacgcctcg ggcgcggtgc tgcgcgtggt gcatcatcaa tgggggcgcg acgaaacgct
     2161 ggagcgcatc ggtgcggagg ccgcgcagcg ccatgaggag gcgcggctga agcgcgtggc
     2221 ggaagagcgg actccgcgta ccgcgatcag catcgatccg cagctgctgg ataattatgt
     2281 gggcgcctat cagctcaagc cccagctggt cttcactgtg acgcgtgaag gagacaagct
     2341 gttggcgaag ctcacgggtc agccaactta cgaggtccat ccatatacgg agcgtgattt
     2401 cttctacacc atcgttgccg cgcagctgtc gttcgtgccc ggtgccgacg gcaaggccag
     2461 cgccgtcgtg ctgcaccaga atggcaagga ccagaccgcg ccgcgtgtcg atttgagcgt
     2521 ggcgcaggcc ctggacgtca agctggccga gcagtcggcg ccgcgcaccg ccgtcaagat
     2581 cgacccaagt ctgctcgatg gctatgtcgg ccgctacagc aatgcggagc tggaaatcac
     2641 cgcgacccgc gaaggcgagc agctgttcgt ccaggtcacc ggttacggcc gatacgccat
     2701 tttcccttac accgagcacg acttcttcgc gaccatccag ccgacccagt acacctttgc
     2761 gacagataac accggcaagg cgacccagat ggtccggcat cggcgtggca aggacgaggt
     2821 gttcattcgg gaggactgat cccgaacgcg tccgtcgtca gccgggcatt cgagcaccca
     2881 agctttgggt tgttgcatga agtataaaat gaggagacta cgatgaagcc aatagctgtt
     2941 atcttgtcgc ccgcaatatc cggcatcgcc ctgtcgtccg atttcacacc ggcgtccgct
     3001 tacaagatgg acggccacta tggcagctcc gatggagggc gctcttattg atacaggatg
     3061 gcaatgagga gggctgcaat ccctcacaca tggagcgcgt atgcagcctc gtgtatccgc
     3121 gattccagcg cgatggcggg ccggttctcg atgtgcctgg cagcgaaagc gcaatgtatg
     3181 cagaccggcg ttcatgtcgg cccttacagc ggcaggcaat acgctgtgat gcgaaagatg
     3241 tagtgatctt ccattcaagc tgacccgaga gagccctcga cgtcagacga tccccgttcg
     3301 gccatgcaca aatacgacag agatccgaaa atgtcggctc gtcccttaat cccgcgccgg
     3361 aaactgttcg acaatccgac tttcttcggc gcgaaattgt cgccggacgg tcgtttgatc
     3421 tcgtggcttg ccccggtcga cggcgtgctg aacgtctggg tctcgccggt cgacagcatt
     3481 gccgccggcg tgccggtcac gcgcaccaag ggccggccga tcaactggca ggattggagc
     3541 ccggacggtc gccacatcat gtttctcaac gatgagaacg gggatgaaaa tctgcacctg
     3601 ttcgtggttg atccccgcaa ttgcgaactc cgcgatctca ctcccttcgc caacatccgg
     3661 gcgataccga cctattggtc gcgcattgcc cccggcaaaa tcgcgatcaa cctcaacgac
     3721 cgtgaccggc gctggcatga tgtttttctg cttgatctcg caaccggcca acgctcgctg
     3781 atctgggaaa atcggcaggg ctttgagttc gtcgggctcg attggcagtt gaagccgcgt
     3841 catgcccgca gccatggctc ggacggcggc gcccggcttt ggcatctcga tggcgagcag
     3901 gtcacgcatt ggcgtgatgt tccattcgat gccagttgga ccacaaggct ctggagtttt
     3961 gacgcctctg gcaagcacct ccatatgctc tcgtgcatcg agcaggataa atcagcgctg
     4021 gtgcagatcg actggtcgag cggcgaacag aaggtcgtgt tcgaaagcca gcgtgccgat
     4081 gttaccgatg cgatattcaa cgcccggacg ttcgagcctg aggctgtctg catcgatccc
     4141 gcgcgacagg aatggagcgc gttgacgcct gcggtcgccg cggaacttgc cttgatcaag
     4201 gcgcggctgc ccggccatgc cttccacgtg cagagccaga ccgatgatga ccgtcgctgg
     4261 atcgtcatga gttacacggc cgagcaggcc gccacctatc atctgcttga tcgggacaag
     4321 cagaccctga ccgagctgtt cacggctcga ccggaattga agccgtaccg tctcgcgccg
     4381 atgcacgccg tcgagggcag gtcgcgcgac gggctcaccc tcgtttcata tttgacgttg
     4441 ccggccgata tcgaaggtga tcgcccggcc gcgccgctgc cgatggtact gatcgtgcat
     4501 ggtggtccgt gggcgcgcga tatcttcggt tttcggggcg accatcaatg gctggccgac
     4561 cggggctatg ccgtgctctc ggtcaattac cgtggctcga cgggcttcgg caaagccttt
     4621 gtcgcggcga gcgaaaaaca acacgcccgc aagatgcatg acgacctgat cgacatggtc
     4681 gattgggccg tcggcgagga gatcgcgcaa aaggacaaga tcgcgatctt cggcgtgtct
     4741 tacggcgggc tggcttcgtt catagccgcg acattcacgc cggaggtttt ctgctgcgcg
     4801 gtcccggtgg tcggtatctc caatctgcaa accctgctgg agtcgatgcc gccctattgg
     4861 gccggctttg ctgaatacat gtatcgcagc tacggcgatc cgcgcaccga ggcaggccgc
     4921 aagctgctcg ccgaacgctc tcccatcaac cgggtcgaga atatcagcaa gcccatgctg
     4981 atctttcacg gcgttaatga cgtgcgctgc aaagttgccg aaagcgatac catcgtcgcc
     5041 gcgatgcggg ccaggaacat ccctgtcacc tacgtggttt acccggatga agggcacggc
     5101 ttccagaaac cgccgaaccg gctttcctat atcgtcatgg cagaggcgtt ttttgcccgg
     5161 catcttggcg gcgccttcga acccgtcggc tccgatcttg aaggctcaag ccacgagatt
     5221 cgcgcgggcg tcgatatcct gacggatttg ggcgtgacct gaccatatca gtccggcttc
     5281 gcccgcacga ccgggccggc cgcgtggccc cggtgacggc tggtgcgatg ctgcaggcta
     5341 ggcgacagcg ggctcgctgg cttcgcgtct gctggcttcg ggtttgctga cttcgacttc
     5401 gtgtggacga accctgaccc agttcctggc aatcagatag gtcagataaa agcacgcggc
     5461 gaggcagacg acataaccgt aggtttgaac cggcggataa atccacggca tcccgtcggt
     5521 caggttatgg atgcagtagg tcgatggcgt accgccctgg accgaaaaat ccgtgaactt
     5581 tcgcgtccag acgtatccag tgcatcccgg tttcgcgggc acgacgttga ggatgaattg
     5641 gacgatcatg aacgtggcgg tcgcgcaggg aacggcgaac agcccgaaca cccaccggct
     5701 cggcttcgtg gcgcctggat ccagatggct catctttccc acgacgaact cgcgcgcgac
     5761 cagcagcgtg tcggactcga ttttcagcga gatcaggaaa agcagcgcaa ggaggggacc
     5821 gaagaccacg ataagaccgg gattgagatc gagcgacacc ccgagcgccg acgccttgcc
     5881 ggggtgatct ccgctgcgcg aaagcgcgca gaggaagatc agcagcaggc tgcggttgcc
     5941 gaaggccctg atgaaaccgg tttcagcgtt cagatacgat agcatggcag cctcattcgt
     6001 cggacacaga taacgtcagt ctggcagcgt ttgcccgggg atcacgacag tctgatttgg
     6061 cgaagctcca ccagctccta gtacaatccg ctgggttgac cggccggacg gcgcgcgcct
     6121 ggcgcagtca cgataggggc ggccggctcc ctggtcacct cgggcacggc ggcatcgagc
     6181 tgcagcaccg ccgccgcggc ggggaacgcc gcgtccgcca tcgcggcatg gccttcggcg
     6241 ctgggatgca ccgcgccgcc atacaccgcg gaaagaacac cccaggtggc gtcgtggatg
     6301 tcggcgggct gactcgaaga ggggagccct tgcggataag tcatcgccgc aaagtagctg
     6361 tcgttggcat cgcggatcca gcgcgcgcgc ggcaggtagg cgcgatattc cccggcgctg
     6421 cggccgcaaa gcatcggctg tgaggcagag gtgacgatat ccggatcgaa actgtcgccc
     6481 ttggccgaaa aacactcccg atcgaattcc ggatcgtttg gtccgcgcgc gcaaaaaccg
     6541 tgatctgcga aggcagcctg atggccatcg acgaaggtca tgcgatcgcc ggccggatcg
     6601 cggcacagga tccccgattg gcacaacgcg agcgccttca actgcggcag gaattcattt
     6661 tgcacatagc cggacacgtt ggccagccgt tgcggctgtg cgttgaacga gggatgaata
     6721 tcgaagccgg cgcgtccgcc ggggcagggc gcgcggtcgg ccagggtcgg gttggcatag
     6781 gatacataga cgacgtgcga catgtcgccg accagggctt tcagcgcctc tctcaatttg
     6841 ccgaaacttt gcggcaggtc ctgcgccagc gccgcgcgcg aatcctctac cgatcccatg
     6901 acgccggtgc ggcgaaacag cacgcgttcg gtcggggtgt cgacgatcac gtccgcgacc
     6961 aggccggaaa agccgatgtc attggcgccg atcgacagca ggaccaggtc gaggttgcga
     7021 tcgggttgac ggcgtttcgc ggcggcgacg gcatcgcgca attcggccaa ttgcgcgttg
     7081 acagtggccc cgcagccggt ggacttggtc ggcgggcatt cccgcgcgcg ttgtccgccg
     7141 aacaacccgt cggggatggt ggcgccggtg catgccagcg gcagataggt caccgcgata
     7201 tgaggatatt gcacggcaag ctgcagcgcg gtgcgggtct gatagctgta gagcgagcga
     7261 tggcaggcgg aattgaacca gagcgcgctt tgccgctgcc acacctgcag cgagtccgga
     7321 gcttcgcagg cccggccgcc tttataaccg gcgcggctcg ggcgataata ttgggcgacg
     7381 gcggttccga ggtaggagcg gaagcagaaa ccctcgtccg acaatgcgat cggccggtcc
     7441 ggattgcctt cgcccgatgc gatgctgtcg ccgaggccgg cgatgaaaat atcgcgcacc
     7501 atgatctcgg tcgcgacacg ctgcggcgcg tcgggtccgc tggagacgtc caccgaagcc
     7561 atcgtgggac ggccatagcg cgcccggaaa cccagcggtt cggcacaatc cagcgtcgat
     7621 tgccgcggcg gcccctcgcc atcgtcgatc gaccaggcgc aggtggcgcc caccgggata
     7681 gtgccggtca gcctgagggt gacgggatga tctgatggcg tcaggtagct ttccttgacg
     7741 ttatcgcggg tgcaaggttc gctgacgcgg cccgtcagat cgatacagag gcggttcacc
     7801 gtgttgcgcg cccagccgcg gccgtcgctc tgcagctcca gcgcttgttc cgagccgaga
     7861 atactgcggc ctcgcgcgct atcggtatgg agcaggaaat cgcgctcttc gcggaacagc
     7921 cgaaagcggt tgcggacctc ccacgaaatc tgcatcgggg cgtccggcgc gccctgcgct
     7981 gccgccggcg cgccctgcgc tgccgccggc gcgccttgcg ccgccgctgc agcggtctgc
     8041 gccgccaccc accgtgtcgg cagcgccccg agcgccccca tgagcagcgc tccgaggatt
     8101 atcgaacgga tgtgaattcg gatcatgacg cgtttgacgt taccgatagg gcggaaataa
     8161 gagagatcac ccagaagcca agggagtcta caagggagtc cagatggcgc gagtccggat
     8221 ggcgcggcga ttagcctttc ctcggccggc acttcaccgg ctgcggcacg gcggacgagg
     8281 gcgggcaggc ctcgtgaagc ttgcggcgtt cctgccaggg cagcacgaca ttgagccaca
     8341 attcgcgggt gcccatgacc gaaatggcga tgccgaaggg gatcacgaaa tacaggatcc
     8401 ggaacaccac cagcgtggcc accaattgtt ccctgccgaa ttcaggcagc gccaccagca
     8461 tcgccgcgtc gaacacgccg atcgagcccg gcgcatgact ggcgaagccg agcagggtcg
     8521 ccagaatgaa caccaccgcc agtgagacaa agtcgattcc cggctgtgcc ggcatcagga
     8581 ggtacatcgc cagcgcgcag aacccgagat cgaccacgcc gatcaggatt tgcagcagcg
     8641 tcaacggcgc cgagggcaat atcactttcc agccgttctg cccgagttcg cgccgctcgt
     8701 cgcccatggc cagccagacc aggtaggaga tgatcgcggc gatgccgccc agcgcgatca
     8761 gccggttgac ggcaggcggc agctgatcca tcgcggaggc tgcccagggg tgcagcgcca
     8821 tgccgatgcc gagcacgaag gtattgccga gccagaacgt caggcccgac agaaagcaga
     8881 ttttggcgac atcgatcgcg ctcaggccgt agtccgaata gatccggaag cggatcgcgc
     8941 cgccggtgaa cacggtggcg ccgatgttgt ggccgatcga atagctggtg aagctcgaca
     9001 gcgccgcgat gcggtagggc acgtttttct tgccaatggt ccgcagcgcg aagaagtcat
     9061 agaacgtcag cgtacagaac gcgccgagca cgcacagcgc ggcgagcccg atgtgggcgg
     9121 gcggtatttc cgtcagcgca gtcaggatca cgccggtatc gacgcccttg agggtgcgga
     9181 ccagcgtcgt gatcgcgaag gcgatgataa ggaggctcgc gacgatcccg agccgtttcc
     9241 agccgatccg tttcttgaaa ccacgcccaa gcgtggtcag cagtcggtgc attcgtcctc
     9301 ccggcagggc ggcaacaagt tggtagccca tcaaagcgat cctcggcaaa cggcggccaa
     9361 tcgctccact tgcaaagacc cataaggcaa aaacgtgtcg ttgtgcag
//
LOCUS       DASWGM010000508        18858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2134711,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000508 DASWGM010000000
VERSION     DASWGM010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18858
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134711"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VGA15_14575"
     CDS             <1..567
                     /locus_tag="VGA15_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9588957.1"
                     /translation="SIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDLH
                     PDQVKLIAKRLGVTDQDVVDMNRRLGGDASLNAPIRDDGEAGEWQDWLVDTSPNQEAI
                     MAEHEEFDHRREALNGAIGVLNPRERRIFEARRLAEDPMTLEDLAAEFGVSRERVRQI
                     EVRAFEKVQSAVKGTIARQEQAALEAAH"
     gene            complement(662..1171)
                     /locus_tag="VGA15_14580"
     CDS             complement(662..1171)
                     /locus_tag="VGA15_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588958.1"
                     /translation="MSASIIWPVAAGHRHLFRVHGIMALRLFPAVVLLLYASVLAASE
                     TPSKPFLEKNSFYLSSAGFRVQVANNPAGQKALRTLPAHRFVVNGVGDAVRYLYAEPQ
                     HCVCIFVGTQQAYDRYRQILSQPLQPTDNVPPDYKTQAGVLLSNQPLRQSTRGDPTTL
                     SDYLSTLHY"
     gene            complement(1365..2669)
                     /locus_tag="VGA15_14585"
     CDS             complement(1365..2669)
                     /locus_tag="VGA15_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9588959.1"
                     /translation="MTHSLRGRLLIGLTLMIVSTGIGAGAVVFQWAFDEAIEMQDAIL
                     TQIGALALNTRFQNDASTDSGVDAEARVTIEELGDRPSGMPDARSHWILRDGLHVVAR
                     DQKPWRFLVRTRPDGSRVAVGQPTEIRDEIARDSAFHTILPFAALAPCLMLVIAIVVW
                     QSLRPMTELAGQLDVRRADDLSRLPLKGTPRELHPFIASINRLLERIQTMMDQQRRFV
                     ADAAHELRTPITAISLQAENLSHVELPAESRDRLIALKNGARRTAHLLEQLLALARHD
                     INSPPEVIVTSLDCCAKEVVSDFMPTAMDRGVDLGFAIIEPSRVRGEAAALSSVVHNL
                     IDNALRHTPQGGRVDIGVYHEGARVILQIEDTGPGIPAADLDRVFEPFVRGSLPAGDG
                     TGLGLSIVKRIVERLEGSVTLENVPKSGLCVIVSFPDAGEGA"
     gene            complement(2666..3328)
                     /locus_tag="VGA15_14590"
     CDS             complement(2666..3328)
                     /locus_tag="VGA15_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9588960.1"
                     /translation="MRVLLVEDDRMVGAAVEQALKDAAYAVDWVTDGRTAIEAAQAES
                     YDVALLDLGLPKADGQEVLRRLRQTGRQLPVIIVTARDGIDDRIDGLDLGADDYLVKP
                     FEIRELLARMRAVLRRQGSGSSSILGNGKISLDPATREATFAGETSLLTAREFAVLRA
                     LLARPGAILSRGDLERHIYGWNEEVESNAIEFLIHTIRKKLGALAILNVRGVGWMVDR
                     AS"
     gene            3957..4715
                     /locus_tag="VGA15_14595"
     CDS             3957..4715
                     /locus_tag="VGA15_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588961.1"
                     /translation="MLNKVPQVTLSFWVIKIMSTTVGETGADYLAVHVGLGSAVTSAV
                     AVSLLLAALFMQLRMRRYVPRIYWLTVVLVSVVGTQITDALTDGLGVSLYASTLAFAL
                     VLAATFAVWYRSERTLSIHTIVTTRRELFYWAAILFTFALGTAAGDLATEALGLGFNV
                     GVVVFGALIAAIAAAYYLGANAVLTFWLAYILTRPLGASLGDLLSQARAYGGFGLGTA
                     YTSIAFLIVIVALVTFVSFDGRRLHHADNAGTTP"
     gene            4784..5179
                     /locus_tag="VGA15_14600"
     CDS             4784..5179
                     /locus_tag="VGA15_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9588962.1"
                     /translation="MKNRELIFVLSLLGALAEPAGVGSLVAASIMTPAEAAMPSKLGD
                     LSSFRTIVTDTTALVDKGDLAGAKTRIKDLETSWDEAEAGLKPRAAAEWHKVDKAIDR
                     ALEALRATAPNAANCKQALGDLLAVMDGA"
     gene            5265..6020
                     /locus_tag="VGA15_14605"
     CDS             5265..6020
                     /locus_tag="VGA15_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588963.1"
                     /translation="MNGISDRSSKVPAVTLAFWVVKILATTLGETGGDAVTMSMNLGY
                     LVGTAIFTAILIVAISVQIAAKRFHPFLYWTVIVATTTTGTTLADFVDRSLGIGYLGG
                     STLLAILLMVSLGLWSRSMGSISTDSITSKKAEMFYWATIMFSQTLGTALGDWMADST
                     GLGYRGGAVVFAIGLAVIAAAYYWTSISRIALFWAAFVLTRPLGATVGDFLDKPLNHG
                     GLALSRYGASVALAELIVVLVATLPQRAGRHPA"
     gene            6164..6511
                     /locus_tag="VGA15_14610"
     CDS             6164..6511
                     /locus_tag="VGA15_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588964.1"
                     /translation="MSIILQSTVVGFSAESIRRLPELEQAMVTHGLEPSQFVISKDRA
                     TPATAPVIGPVFYDYTVFAGDENFTVTEPDDIKFLEYFYRRIARNATTPAPQGRRRRG
                     LISRLLSWMTQPI"
     gene            complement(6530..6814)
                     /locus_tag="VGA15_14615"
     CDS             complement(6530..6814)
                     /locus_tag="VGA15_14615"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9588965.1"
                     /translation="MKLEWSDEALADLDRFVEFLGRERPSLAKIVATEIIDKVQVLSE
                     HPQLGRPIAGRQEYRQIVLQVLGAAYVFQYRFDGRRLVMLRVYHARESRK"
     gene            complement(6811..7143)
                     /locus_tag="VGA15_14620"
     CDS             complement(6811..7143)
                     /locus_tag="VGA15_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588966.1"
                     /translation="MLRMPCDTFLDSGRVFGYNPQMKTQMIEIDDDTAMALKQRAAER
                     GLSVPELLAELVTLEAGPVDTDVDDIVELDRRWKAFEVQGSVAANDDVVRWLQTWGTP
                     AFRSWRNR"
     gene            complement(7167..7790)
                     /locus_tag="VGA15_14625"
     CDS             complement(7167..7790)
                     /locus_tag="VGA15_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX9588967.1"
                     /translation="MNFRSLASALCVVASFTSVYAQTSDLTTIARLSGTPDIPGLKMI
                     WLAPWGEVSKAHPWRNIIVHQTEGPAGSARGGAQEQAKNPTRRGVTVWVETDGTVYWA
                     VPENLVPTHGDGANRNDHKYIDNGPTFHQVIGSNSIGVEFAGNYPDVTRGATEAQVAA
                     WRILVALLRTRYGIPLERVYAHNWIDYKDARYCEGCALATLARQWGE"
     gene            complement(7828..9054)
                     /locus_tag="VGA15_14630"
     CDS             complement(7828..9054)
                     /locus_tag="VGA15_14630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX9588968.1"
                     /translation="MLSVPTLWTAYVVNFLALGLVWAYVVRSYPKFGAARFWTGSALT
                     AAAGAVLAMLRMFMDSLLPLLVAATILIFAACLAEMGVKRFYDRPVSWRGTALISGLS
                     FAGMTFFLVGYDSTAMRVLIYSLGQCLPFALTLRLLLSPQHGRSSPGARLAAIVAILI
                     TAIYVVRVAANLLPVGAGLTFAQFSPLQSVLVLALMFLSMVWNFGCLLMAMDRLRNEV
                     ADLALLDDLTGVGNRRHLLQRLTEECARSERSGEPFALLVVDLDGFKAINDTHGHAAG
                     DSCLQHFTLMAQTRLRPSDMLARSGGDEFCIVLPASTLREGAMIARRILDVCRADAEA
                     CVGADIPIALSIGVAQWTREIGTFPDRLMAAADHALYAAKKDGKNCHAVYDPTLPLSP
                     EITAQAMPNLALRQRA"
     gene            complement(9805..10416)
                     /locus_tag="VGA15_14635"
     CDS             complement(9805..10416)
                     /locus_tag="VGA15_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX9588969.1"
                     /translation="MSLAFLLTSLIVIVSPGTGVLYTLAVALTRGSRASIAAAFGCTL
                     GIVPHICAAMLGLAAVLHTSALAFAALKWCGVIYLLVMAWQALRERGALALDTRLDRR
                     SGKRVIVTGFLINILNPKLSIFFLAFLPQFVAANEGHPLLRMLELSGAFMAMTFAVFL
                     LYGLFAASVRDRIVTRPRVMTWLRRAFAGGFAALGARLAFAER"
     gene            10548..11402
                     /locus_tag="VGA15_14640"
     CDS             10548..11402
                     /locus_tag="VGA15_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9588970.1"
                     /translation="MTAIRGSAAAVTGAASGIGRALALELAVRGCDLALADRDEAGLQ
                     ATAAEIGRAAPQKVTVHRVDVSEPQQIAAFAQAATLAHPGLNIVVNNAGVALLGQFNE
                     IDQAQMDWLMNINFWGVVHSTRAFLPHLARQREAHIVNLSSIFGIIAPPGQTAYAAAK
                     FAVRGFSESLRHELQMAASPVRLSVVHPGGVSTNIVRNSRAGSGVTDNARRAQTIERF
                     DAVARTTPAAAALRIIAGIEKNQPRILIGNDARFMDLLQRFRPGTYWAVLARRIEKAA
                     KAGKPGAP"
     gene            complement(11562..12362)
                     /locus_tag="VGA15_14645"
     CDS             complement(11562..12362)
                     /locus_tag="VGA15_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX9588971.1"
                     /translation="MLRIAIVLCAITLATPSFAARCGGDFNTFVASMSAEAQAAGVSP
                     SVTDAAFGGVTQDMAVLNFDRRQRYTFNKSFEQYISTRVGAGRINGGRAMLLRHASLL
                     SRIEARFGVPPQILVAIWGLETDFGKGDMGKLPVVRTLATLAHDCRRSELFQGELLAA
                     LKIVQRGDLPLRDLIGAYAGEIGQTQFLPSSYVKYGVDFDGDGRVDLRHSVADVLAST
                     ANLLHTNGFKNGAPYGEGTANFEAMREWNHATIYRKTIGYFADQLVGR"
     gene            complement(13754..14020)
                     /locus_tag="VGA15_14650"
     CDS             complement(13754..14020)
                     /locus_tag="VGA15_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX9588972.1"
                     /translation="MIKVAKIKCLGGHRLRATFSDGMAGEYDFSAIVSGSGPMVEPLR
                     DPAFFARVFLEDGAPTWPNGFDVAPGWLRREIEAAGALARDAAA"
     gene            complement(14177..14728)
                     /locus_tag="VGA15_14655"
     CDS             complement(14177..14728)
                     /locus_tag="VGA15_14655"
                     /inference="COORDINATES: protein motif:HMM:NF037889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX9588973.1"
                     /translation="MGTIVDKLYGEYSAIVSMMDQYNAVSFRNTLNENFRKTLLLCAA
                     SHFEFRITSDVVSFCAEVAGGNSMIPTLVKNKAVSRQYHTWFEWKANNANAFFNMFGD
                     DFRLHMAEIVKLDQNMAKSISDFIEIGRERNRLVHQDYGSFFLEKSAEEIFRSYVSAM
                     VFVDFVPKALRDFSAKLRAKSVA"
     gene            complement(14742..15332)
                     /locus_tag="VGA15_14660"
     CDS             complement(14742..15332)
                     /locus_tag="VGA15_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588974.1"
                     /translation="MYEVFNRLNTGGINLRPQEIRTSMYHSKFYDMLYRVNTDSRWRT
                     ILNSPEPDLHMKDIEILLRGLAMLISGHEYAPSMVRFLNQFSRMCESHDSKQNEYLQK
                     LLTSFLEACEDLPDDAFVNPKNNRFNIALFEAVFTATCDEAFKKRRLLKGKIDPDEVS
                     ALERDRQFVSASLEGTTRTTNVGTRLKRARALISTL"
     gene            complement(15431..15898)
                     /locus_tag="VGA15_14665"
     CDS             complement(15431..15898)
                     /locus_tag="VGA15_14665"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX9588975.1"
                     /translation="MAEWFDDYADEEDELQIDEYDITATPNDFNVSTLFNFIESGAVR
                     IPGFQRNFVWDQVRASKLIESLILGIPVPQLFLYEQARNRFLVIDGQQRLMSIYYFIK
                     RKFPRKDKRVEIRAIFDREGRIPDEVLHDDVFLMTSNSSSQKNCQIKLINFMD"
     gene            16242..16751
                     /locus_tag="VGA15_14670"
     CDS             16242..16751
                     /locus_tag="VGA15_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588976.1"
                     /translation="MNIRRGLWRTWIFLTALWVIGTVTLAYFVLLESVASRKYQYVYN
                     MRSDVPDPNKVDWTKDFYSLMQSPSKEKLASTFDIQTYQYATTWDEDVKKGMLITVEF
                     PDKSHLYMSAQMTQVDRDYVAKAFWNQRWERWGIETLPWVAGAIVPPIILLLLGSFMV
                     WVGRGFTRD"
     gene            complement(17238..17483)
                     /locus_tag="VGA15_14675"
     CDS             complement(17238..17483)
                     /locus_tag="VGA15_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588977.1"
                     /translation="MIGTELPEWRKRNHFTQDALRMALGLGSRQTIITWEKSTEKLPR
                     MVELALLALERLPDQRTALGYSYATADYPVQRARPTF"
     gene            complement(17859..18125)
                     /locus_tag="VGA15_14680"
     CDS             complement(17859..18125)
                     /locus_tag="VGA15_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX9588978.1"
                     /translation="MIKVTKIKHLGGYRLHATFSDGTAGERDFSVLVAEAGPMGEPLR
                     DPAYFARVFLEDGAPTWPNSFDMDPEWLRREIEAMGGLARDDLA"
     gene            complement(18288..>18858)
                     /locus_tag="VGA15_14685"
     CDS             complement(18288..>18858)
                     /locus_tag="VGA15_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9588979.1"
                     /translation="YKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSR
                     QMLNEIGREPTPEELAEKLGMPLEKVRKVLKIAKEPLSLETPVGDEEDSHLGDFIEDK
                     NAILPIDAAIQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTR
                     ERIRQIEAKALRKLKHPSRSRKLRSFLDN"
BASE COUNT         3843 a         5716 c         5609 g         3690 t
ORIGIN      
        1 tcaatacaag agtatatcct gcgttcctgg tcgctcgtga aaatgggcac caccgcgaac
       61 cagaagaagc tgttcttcaa cctgcgcaag gccaagagca agatctcggc gctggacgag
      121 ggtgatcttc accccgacca ggtgaagctg attgccaagc ggctcggcgt gacggatcag
      181 gacgtggtcg acatgaaccg ccgtctcggc ggcgatgcct cgctcaacgc accgatccgc
      241 gacgacggcg aggccggcga atggcaggac tggctggtcg atacctcgcc caaccaggaa
      301 gccatcatgg ccgagcacga ggagttcgat catcgccgcg aggcgctgaa cggcgcgatc
      361 ggcgtgctca acccccggga acgccgcatc ttcgaggcgc ggcgtctggc ggaagacccg
      421 atgacactgg aagacctcgc cgccgaattc ggcgtgtcgc gcgaacgcgt gcggcagatc
      481 gaggtccgcg ccttcgagaa ggtgcaatcg gccgtcaagg gcacgattgc ccggcaggaa
      541 caggccgcac tggaagccgc gcattaattt tcgcgggttg tgaggtatcg aaacacgaaa
      601 gccggcggcg acgccggctt ttttgttgac gctgtcgcag gtggcggtcc cgggcggccc
      661 ctcaataatg aagagtgctg agatagtccg acagcgtggt gggatcgccg cgcgtactct
      721 gccgcagcgg ctggttgctc aacagcacgc cggcctgggt cttgtaatca ggaggcacat
      781 tgtcggtcgg ctgcagcggc tggctgagga tctggcggta cctgtcatag gcctgttggg
      841 tgccgacgaa gatgcagacg caatgctgcg gttcggcata aagataacgc acggcgtcgc
      901 caaccccgtt tacgacgaag cggtgcgccg gcagcgtgcg cagcgctttt tgcccagccg
      961 gattgttggc gacctggaca cgaaaaccgg cggacgacag ataaaagctg ttcttttcca
     1021 ggaacggctt gctcggcgtc tccgaggcgg ccagaaccga ggcgtagagc aacagcacaa
     1081 cggccggaaa caggcgcaag gccatgatgc catggacccg aaatagatgc cgatggcccg
     1141 ctgcgaccgg ccagatgatc gatgctgaca ttgcaaattc ccctgcctga gcgttgcctg
     1201 ttgcgccggc tataacgccg cgagcagtgc gccaagattg tgttgcacct cgagcggcat
     1261 ccagccgacc aacaatagta gggggcatat gcgacccaat cccacgcagg cagcacccgc
     1321 atgggggcaa aagccggcgg cgacgccggc tttgtttgtt gctcctatgc cccctcgccc
     1381 gcatcgggaa aggaaacgat cacgcaaagg ccggactttg gcacgttctc cagggtcacc
     1441 gagccttcga gccgttcgac aatgcgcttg acgatggaga gtccgaggcc ggttccgtcc
     1501 ccggcaggca ggcttccgcg aacgaacggt tcgaataccc gatcgagatc cgccgccgga
     1561 atgccggggc cggtgtcttc gatttgcaaa atgacccgcg cgccttcgtg gtaaactccg
     1621 atatcgaccc ggcccccttg cggcgtgtgg cgcagcgcgt tgtcgatcag attgtggacc
     1681 acggacgaga gcgccgccgc ctcgccgcgc acccgtgaag gctcgatgat tgcaaacccg
     1741 agatcgacac cacggtccat cgccgtgggc atgaaatccg aaacgacttc cttggcgcag
     1801 caatccagcg atgtgacgat aacctcgggc gggctgttga tgtcgtgccg cgccagcgcg
     1861 agcaattgct ccagcaggtg agccgtcctg cgcgcgccgt ttttgagcgc gatcagccgg
     1921 tcacggctct cggcgggaag ttccacatga ctcaagttct ccgcctgaag actgatcgcg
     1981 gtgatgggcg tccgaagctc atgggcggca tcggcaacga agcgccgctg ctggtccatc
     2041 attgtctgta tccgttcgag cagccggttg atcgaagcga tgaacgggtg gagttcacgc
     2101 ggggtgccct tcaggggaag tctgctcaga tcgtccgcgc gcctgacgtc cagctgtccg
     2161 gcgagttccg tcatcggtcg cagcgattgc catacgacga tcgcgatcac gagcatcagg
     2221 cacggcgcca aagctgcgaa tggcagaatc gtatgaaagg cactgtcgcg cgcgatctcg
     2281 tcgcgtatct cggtgggttg gccgacggca acccggcttc catccggccg cgtccgcacc
     2341 aggaaccgcc atggcttctg atcgcgggcg acgacatgca aaccgtctcg aaggatccag
     2401 tgcgagcgag cgtcgggcat gccgctcggc ctgtcgccta attcctcgat ggttacccgc
     2461 gcctccgcgt cgacgccgct gtcggtcgac gcatcattct ggaagcgggt attgagcgcc
     2521 agggccccga tctgggtaag gatcgcgtcc tgcatttcga tcgcttcatc gaaggcccac
     2581 tggaaaacca cggcacccgc gccgataccc gtcgagacga tcatgagcgt aagtccgatc
     2641 aggagacgtc cgcgcaggga gtgggtcatg aggcgcggtc caccatccag ccgacgccac
     2701 ggacattgag gatcgcgagg gctccgagct tcttgcgtat cgtatggatc aggaattcga
     2761 tcgcgttgct ctcgacttcc tcattccatc catagatgtg gcgttcgaga tcgccgcgag
     2821 acagaatcgc acccgggcgc gccagaagcg cccgaaggac cgcgaactcg cgcgcagtaa
     2881 gcagcgacgt ctcgccggcg aacgtagcct ccctcgtcgc cggatcgagg cttatttttc
     2941 cgttgccgag tatagaagac gagccgctgc cctgacgccg gagaacggcg cgcatccgcg
     3001 ccagcagctc gcggatctcg aagggtttca ccagatagtc gtccgctccg aggtcgaggc
     3061 catcgatacg gtcgtcgatg ccgtcgcgcg ctgtcacgat aatcaccgga agctgacggc
     3121 cggtctggcg gagccggcgc aagacttcct ggccatcggc cttgggcagg ccaagatcga
     3181 gaagcgcgac gtcgtagctc tcggcttgcg cggcttcgat cgccgttcgg ccatcggtga
     3241 cccaatcgac cgcataggcg gcatccttca gcgcctgctc cacagcggct ccgaccatac
     3301 gatcatcttc gacgagcaga acgcgcatgg tttatcctgt cggcacatcc gccgagcgca
     3361 atgggcccgc catgatcgcg tcggtctgaa agttgctaat ccggtttgtc gtctttggta
     3421 ccaggcctgc cctcggctgt tgcaatgact gtatctttgt cggcatcgac ttccacatcg
     3481 aaaatgtttg accggttgcg gacctcgaat tcatagaccc catcggccgt cttccaacgg
     3541 cctgtccctg aagtcgtttc tgcgcagcga taacggcggc agccagcgaa ttggcggcat
     3601 ggggagcggc gaccgcatgc ttcgttgaga gcgtagcggt tccggcgaga gcgcgttccg
     3661 acagaaaaat gtcggttgca gcccaaggca cgagcgtaga tcggtgcatg tcgtggttcc
     3721 cccgatgcct attccaacct gaattctgtc actccgaaac ttagggccga cttagcggcg
     3781 atcccgcgcg cattgtgaac gttgaacggc cggacaccaa aacgacccct cattctaagt
     3841 cgccgctaag tgaggaccgc caaggttttg cgtgacggta cgccggtctt cgaacgcgac
     3901 ccaattgatg aagggatatc cgtgtgcgcg atcctgttag tcaacgtccc taccaaatgc
     3961 tcaacaaggt tccgcaagtc acgctttcgt tctgggtcat caagatcatg tcgaccaccg
     4021 tgggtgagac cggcgcggat tatctggcgg tgcatgtcgg actagggtcc gcggttacca
     4081 gtgccgtcgc cgtgtcgctg ctgctcgcgg cgttgtttat gcaattgcgg atgcggcgct
     4141 atgtgccgcg gatctattgg ttgacggtcg tgctggtgag tgtcgtcgga acccaaatta
     4201 ccgatgcgct caccgatggg cttggcgtta gcctgtatgc cagcactttg gcattcgcat
     4261 tggttttggc cgcgacattc gcggtctggt accgcagcga acgcacgctt tccattcaca
     4321 ccatcgtcac cacgcggcgt gaactgtttt actgggccgc cattcttttt acgtttgcac
     4381 tgggcaccgc ggccggcgac ctggccaccg aagcgcttgg tctcggtttc aacgtcggcg
     4441 tcgtggtgtt tggcgcgctc atcgccgcga tcgctgcggc ctattatttg ggcgcgaacg
     4501 ccgtgctcac tttttggctc gcctatatcc tgacaaggcc gctgggtgcc tcgcttggcg
     4561 atctgttgtc ccaggcgcgc gcctatggcg gtttcggtct cggcaccgct tacaccagca
     4621 tcgccttcct catcgtcatc gtcgcactcg tgacgttcgt cagtttcgat ggacgacgcc
     4681 tgcatcacgc cgataacgcg ggcacaacgc cataatgcaa atcgttcgat ttcaccaagc
     4741 gcttgcacat gtcgtgcgag ccactcagca caggagacaa gccatgaaaa accgggaact
     4801 gatttttgtc ttgagcctat tgggcgccct tgccgaaccc gccggcgtcg ggtcgttggt
     4861 agccgcttcc atcatgaccc cagccgaagc tgcgatgcca tcgaaactcg gcgacctttc
     4921 gtcgttccgg accatcgtga cggataccac agcgctcgtc gacaaaggcg atctcgccgg
     4981 cgcaaagacc cgcatcaagg atctggaaac gtcgtgggac gaggctgaag caggattgaa
     5041 gccgagggcg gctgccgagt ggcacaaggt cgacaaggcg atcgaccgcg ccctcgaggc
     5101 cttgcgcgcc actgccccga acgccgccaa ttgcaagcaa gccctcggcg atctgctcgc
     5161 ggtcatggac ggggcttgaa acaacggatc gcgcccggcc gagagaatca acacgtgttg
     5221 caaccatcct cccggcactg tcgggctcaa tcgggataca aatcatgaat ggcatttccg
     5281 accgctccag caaggtcccc gcggtcacgc tcgcattttg ggtggtaaaa atccttgcga
     5341 ccacgctggg cgagaccggc ggcgacgcgg tcaccatgtc catgaacctc ggatatctgg
     5401 tcggaacggc aatcttcaca gccattctta tcgttgcgat cagcgtccag atcgcggcaa
     5461 agcgtttcca tccgtttctg tattggaccg tcatcgtcgc cacgacgacc acgggcacta
     5521 cgctagccga tttcgtcgac cgctcgcttg ggatcggcta cctcggaggt tccacgctcc
     5581 tcgccatctt gctgatggtg tcgcttggcc tctggtcccg ttcgatgggt tcgatttcaa
     5641 ccgactcgat cacatccaag aaagccgaga tgttctattg ggccaccatc atgttctcgc
     5701 aaacgctcgg caccgcgctt ggagactgga tggccgattc caccggcctt ggataccgtg
     5761 gcggagccgt ggtttttgca atcggtctcg ccgtcatagc tgccgcctat tactggacct
     5821 cgatctcgcg catcgccctg ttctgggcgg ccttcgtgct cactcggcca ctgggtgcaa
     5881 ccgtcggcga tttcctggac aagccgctga atcatggcgg actggcgctc agccggtatg
     5941 gtgcgtcagt ggcactggcg gagctgatcg tcgttttggt tgcaacgctg ccgcagcggg
     6001 ctggccgaca tccggcatga cagggcggca cggaaacaga atacgggttg gttgccgacc
     6061 gtcatcgagc acaccgcccg aaccttcagc cctttccggc cgaccaagat acctggagat
     6121 attgcgccat tttcggacgc ggggcgttct cacgaggcag gctgtgtcga tcattctgca
     6181 atccaccgtc gtcgggtttt ccgccgaatc catccgcagg ctgccggagc tcgagcaggc
     6241 gatggtcact catgggctgg agccgtcgca attcgtgatc tcgaaggacc gcgcgacgcc
     6301 agccacggcg ccggtgatcg ggccggtctt ctacgattat actgtgttcg ccggcgacga
     6361 gaatttcacc gtcaccgagc cggacgacat caagttcctg gagtattttt accggcgcat
     6421 cgcacggaac gcgaccacgc cggcgccgca aggcagacgg cgtcgcggcc tgatcagccg
     6481 gctcttgagc tggatgacgc agccgatttg attggggggt ggcgcgcctt cactttcgcg
     6541 attcgcgagc atgatagacg cggagcatca ccagacgccg gccatcgaag cgatattgaa
     6601 acacataagc cgcgccgaga acctgcaata cgatctggcg atattcctgc cgaccagcaa
     6661 tcggccgacc gagctgcgga tgttctgata atacctgcac cttgtcgata atttcagtgg
     6721 cgacaatctt ggcgagcgac gggcgctccc gaccaagaaa ttctacaaat cgatcgaggt
     6781 cggcgagagc ctcgtccgac cattcaagct tcatcgattg cgccagctcc ggaaggccgg
     6841 cgtgccccac gtctggagcc accgaacgac atcgtcattc gccgcgactg aaccttgcac
     6901 ctcaaacgct ttccagcggc gatcaagctc aacgatgtca tcgacgtcgg tatcgacagg
     6961 acccgcctca agcgtgacca attccgccaa cagttccggc acgctcagac cgcgttccgc
     7021 ggcgcgttgc ttgagcgcca tcgcagtgtc atcatcgatt tcgatcatct gggtcttcat
     7081 ttggggatta taaccgaata cgcgtcccga atccagaaag gtgtcgcacg gcatccggag
     7141 caagatggct gcatcctcgg cctattctac tccccccatt gccttgccag cgtcgccagc
     7201 gcgcagcctt cgcaatagcg ggcatccttg tagtcgatcc aattgtgcgc atagacccgc
     7261 tcgaggggaa tgccgtagcg cgtccgcagc aaggctacca gaatccgcca cgccgcgacc
     7321 tgcgcctcgg tcgcaccgcg cgtcacgtcg ggataattgc cggcgaactc caccccgatc
     7381 gaattgctgc cgatcacctg atgaaaggtc gggccgttgt cgatgtattt gtggtcgttg
     7441 cggttggcgc cgtcgccatg ggtcggaacc agattttccg gcaccgccca gtacaccgtg
     7501 ccgtcggttt cgacccagac cgtgacgccg cgccgggtcg ggtttttcgc ctgctcctgc
     7561 gcgccgccgc gtgcggaccc ggccggcccc tcggtctggt gcacgatgat attgcgccag
     7621 ggatgggctt ttgacacctc gccccacggc gccagccaga tcatcttcag accgggaata
     7681 tccggtgtgc ccgacaaacg agcgatcgtg gtgaggtcgg acgtctgcgc gtagactgaa
     7741 gtgaacgatg cgacaacgca gagcgccgag gccagcgagc ggaaattcat gagtgccggg
     7801 atccaatgaa cggcctgatt agcacgctca cgcgcgctgg cgcagggcca gattcggcat
     7861 cgcctgggct gtgatttcag gcgacagcgg cagcgtggga tcatagacgg catggcagtt
     7921 cttgccatcc ttcttggcgg cataaagcgc atggtctgcg gcggccatca gccggtccgg
     7981 gaaggtaccg atctcccggg tccattgcgc gacgccgatc gacagcgcga tcgggatatc
     8041 cgctccgacg caggcctcgg cgtccgcgcg gcacacatcc aggatgcggc gcgcgatcat
     8101 cgcaccctcg cgcagcgttg aagcgggcag cacgatgcag aactcgtcgc caccggagcg
     8161 cgccagcatg tcgctgggcc ttaggcgcgt ctgcgccatc agggtgaaat gctgcaggca
     8221 gctgtcgccg gcagcatggc catgggtgtc gttgatcgcc ttgaagccgt cgaggtcgac
     8281 caccagcaat gcaaacggtt caccgctacg ctcggacctg gcgcattcct cggtcaggcg
     8341 ctgcaacaga tggcggcggt tgccgacacc ggtgagatcg tcgagtagcg ccagatcagc
     8401 gacctcgttg cgcagccggt ccatcgccat cagcaggcag ccgaaattcc acaccatcga
     8461 caggaacatc agcgccagga ccagaaccga ctgcaacggg ctgaactggg cgaaggttag
     8521 cccggcgccg acaggcagca gattggctgc gacgcggacg acatagatgg cggtaatgag
     8581 aatggcgaca atggctgcga gccgcgcgcc ggggctgcta cggccatgct gcggcgacag
     8641 caacagcctt agtgtcagcg cgaacggcag acactggccc aacgaataga tcagtacccg
     8701 catcgccgtg ctgtcgtaac cgacgaggaa gaacgtcatg ccggcaaagc ttagtcccga
     8761 tatcagcgcg gtgccgcgcc aggacaccgg ccggtcgtag aaccgcttga cccccatctc
     8821 ggcaaggcag gcggcgaata tcaggatggt ggcggcgacc aggagcggca gcagcgaatc
     8881 catgaacata cgcagcatcg ccagcaccgc gccggcagcc gccgttaatg ccgaaccggt
     8941 ccagaaccga gccgccccga atttcggata actgcgcacg acataggccc agaccaggcc
     9001 gagcgcgaga aaattgacga cgtacgccgt ccacagcgtc gggacgctca gcattgcgaa
     9061 gccagaacac gcacggtcca gtcccctgtc gtcgaagagc ccgcttaggg caagcctatc
     9121 tttcgaagcg taacatgcag ctatgtcgct taacgggctc tgatccgcca cggcgcaact
     9181 tcgcagtgtg gttttgaatt gatgaagacg gcgcgcgcca gttccggatc gatgcagaat
     9241 gcgccggcgc gttggtacct cgttaactct aaagggcgtc gcgaatggtg cgatgatgct
     9301 cttacatagc gtcctcgccg gacttgatcc ggcaatccat cttgcttgca gattgatgga
     9361 tgcccgggct ttcacgcttc gcctgaacac gccgcgtgaa aaattgcagg aaaagcgcat
     9421 caaaatgcat aacagctgtg gataacgcga tgacaccggc gtgacagcgc gaggcagcac
     9481 gccgcgaatc tgttcggatg atgagtgagg ctgttgcgag gctagccctc cccgagcatg
     9541 cctcgtgtcg cgtttccatc cattaaactc aaaagaggat gctatggcta agaaagcgaa
     9601 gaaggcgaaa aaggcgaaga aggcgaaaaa ggccaagaag gccaagaagt aaccttcaag
     9661 cccgggtgga gcctcgctcc gcccgggcac ccgctaccag gttcaacatg ttcaccaaag
     9721 cggcgggcct cgagcccgcc ttttgatttg ggtgcttcca attcatcgtc gtcgcccgat
     9781 caagcggggc atgacggtcg gaagtcaccg ttccgcgaac gccagcctgg cgcccagcgc
     9841 ggcaaagccg ccggcgaagg cgcggcgcag ccaggtcatc actcttggcc gcgtcacgat
     9901 gcgatcgcgg accgatgctg cgaacaggcc gtagagcaaa aataccgcaa acgtcattgc
     9961 catgaacgcg ccgctcagct ccagcatccg cagcagaggg tggccttcat tcgccgcgac
    10021 gaactgcggc aggaacgcca ggaagaagat cgacaatttc ggattgagga tgttgatcaa
    10081 aaatccggtg acgatcaccc gcttgccgga gcgccggtca agccgggtat cgagcgcgag
    10141 cgccccgcgc tcgcgcagcg cctgccacgc catgaccaac agatagatca cgccacacca
    10201 cttcagcgcg gcgaaagcca atgcgctggt gtgcagcacc gcggcgaggc cgagcatcgc
    10261 ggcgcagata tgcgggacga tcccgagcgt gcagccgaac gccgcggcga tactggcccg
    10321 cgatccgcgc gtcagcgcca ccgccagcgt gtagagcacg ccggtgccgg gcgagacgat
    10381 cacgatcagc gaggtcagca ggaacgcgag ggacatcgct cttgcccata tcacgtcgtc
    10441 ggacagcggt gaagcgcgcg caagcacgaa ggtcgcccgg ttgccaaagc ctggcagtcg
    10501 tataagggtt gccgcgcacg cctttcccga catggagttg acattcaatg accgcgatcc
    10561 gtggctccgc cgccgccgtg accggcgctg ccagcggcat cggccgggcg ctggcgctcg
    10621 agctggcggt gcgcggctgc gatctggcgc tggccgatcg cgacgaggcg gggctgcaag
    10681 cgaccgccgc cgagatcggc cgggcggctc cgcaaaaggt cacagtccac cgcgtcgacg
    10741 tcagtgagcc gcagcagatc gcggccttcg cgcaagccgc caccttagcc catccggggc
    10801 tcaacatcgt cgtcaacaat gccggcgtcg ccctgctcgg ccagttcaac gaaatcgacc
    10861 aggcgcagat ggactggctg atgaacatca atttctgggg tgtggtgcat tcgacgcgtg
    10921 cgttcctgcc gcatctggcg cgccagcgcg aggcccacat cgtcaacctg tcgagcattt
    10981 tcggcatcat cgcgccgccg gggcaaacgg cttacgccgc ggcgaaattc gcggtgcgcg
    11041 gcttttcgga aagtcttcgg cacgaactgc agatggcggc aagtccggtg cgcttgtcgg
    11101 tggtgcatcc cggcggcgtc tcgaccaata tcgtgcgcaa ctcccgcgcc ggcagcggcg
    11161 tcaccgacaa tgcgcgccgc gcgcaaacga tcgaacggtt cgatgcggtg gccaggacca
    11221 cgcccgccgc cgccgcgctg cgcatcatcg ccgggatcga gaaaaaccag ccgcgtatcc
    11281 tgattggcaa cgacgcccgc ttcatggacc tgctgcagcg ctttcggccc ggcacctatt
    11341 gggcggtgct ggcgcgccgg atcgagaagg cggcgaaggc ggggaaaccc ggtgccccat
    11401 agcgcataac cctacacgct cgtcattccg gggcatcgcg aagcgatgag cccggaatcc
    11461 ataaccacga cagggagtat ggattccggg cctgcgcgaa gtggcgcatc ccggaatgac
    11521 ggtggttatg tcgatgcgcc gtccggctta ggaaggaacg ctcaccgccc caccagctga
    11581 tcggcgaaat agccgatggt cttgcgatag atggtcgcgt gattccattc gcgcatcgcc
    11641 tcgaaattcg cggtgccttc gccgtaaggc gcgccgttct tgaagccgtt ggtgtgcaag
    11701 aggttggcgg tggaggcgag cacgtcggcc acgctgtggc gcaggtcgac acggccgtcg
    11761 ccgtcgaaat cgacgccgta tttgacatag gacgacggca agaattgcgt ctggccgatt
    11821 tcgccggcgt aggcgccgat caaatcccgc aatggaaggt cgccgcgctg cacgatcttg
    11881 agggcggcga gcaattcgcc ctggaacagt tccgagcggc ggcaatcatg cgcgagcgtg
    11941 gccagcgtgc ggaccaccgg cagcttgccc atatcgccct tgccgaaatc ggtctccagc
    12001 ccccagatcg cgaccaggat ctgcggcggc acgccgaacc gcgcctcgat ccgcgacagc
    12061 agcgaggcgt ggcgcagcag catcgcccgg ccgccgttga tgcggcccgc gccgacgcgg
    12121 gtcgagatat attgctcgaa cgacttgtta aaagtgtagc gttggcggcg gtcgaaattc
    12181 aacaccgcca tatcctgcgt cacgccaccg aacgccgcgt cggtcacgct tggcgagacc
    12241 cccgccgcct gcgcctccgc cgacatgctc gccacaaaag tattgaagtc gccgccgcag
    12301 cgcgcggcaa aggacggggt ggcaagggtg atggcgcaga ggacgatggc aatgcggagc
    12361 atacaagctc tttctgttca tgaattggcc cggcgaagtg gcgtttcata gcacggcttg
    12421 gaccatcgtt cgagaggtca gtgcttcgcg ggcgcaagtg acggttcccg tatgtagtgc
    12481 cagcggtcac aatttcggac attctgcgag ggcaatccat agtaacaaag taagtctggg
    12541 tcgctttgtc gcttcgttcg tcgcgaaggg accgagatgc tcggcatcac ctctcccctt
    12601 gtgggagagc ataggccgcc ttcggcggcc gttcttggaa aaggacgccg aagcgaagct
    12661 tcggctacgt cgcatcgata gatgcgatcc gggcgagggg ttacggccca tcgataggcc
    12721 gcgccccctc accccaaccc tctcccacaa ggggagaggg agcgcgccgc cgctgaagca
    12781 aattccggat ttaattttca aacagccaag acgattcagt tggaaccgtc attgcgagcg
    12841 aagcgaagca atccacagcc acgaggtaag tctggattgc ttcgcttcgc tcgcaatgac
    12901 ggcgctctaa gtccagatac gacttcacgc cctcgcaacg caaagcgccc gagtcgtgca
    12961 tttatctctc gcccttgagg gcgtggggaa cgccgggtgc ctgctgcacc cgcagcctcg
    13021 cgtgcacttt gtattggtag aaagcacacg agtaacaacg agtacaccgg aacacccggc
    13081 attcccgcac gcaatggttt taacggctta tgtcgtgctc tccccggtga ccgggctctt
    13141 ttgccaccgt cgtcagcgga tatagttttg tctaagcccg gtcgggccga cgcaactccg
    13201 cgaacttgac gccagcgtcg gggcgtcagg accacacgat tttgccgtcc gcagcaatcg
    13261 tctctcgtcc gcgtgctgtt gaccgctcac ggattcaaag aacccgccct gcgatcccgt
    13321 cgcgcgcaaa acgctgccgc gtccaccgca tcccatcccg cgtccgtgac gatcgcgata
    13381 cgcccctcga gtgggacgag acggtgtcgg atataggggt gatttgggta aagcgaaaac
    13441 agcaatattt ttgcctatgg gactcgacag cccaaatcac accaaatctg gcccgtcggg
    13501 ctggcgactt ttttacacga cgtcgtccgc cgtcgcgacg gcatcgaaaa attcggcacg
    13561 tcgggcaaat cagcgagcgt agcccgggcc tcaatgcttc ggcggataaa gcagtacgta
    13621 gggcgggttt tagccgaagg cgtaacccgc cgtcttgggc tggcgaatgg cggattacgc
    13681 cttcggctca atccgcccta cgagctctgt gttcatgccg aagcgcatgc gcagcacgcg
    13741 ttcttcgcgc cgcctacgcc gccgcgtccc gcgccagcgc gccggccgcc tcgatctcgc
    13801 ggcgcagcca gccgggcgcg acatcgaaac cgttaggcca ggtcggtgca ccatcttcga
    13861 ggaatacgcg tgcgaaaaac gcgggatcgc gcaacggttc gaccatggga ccgctgccgc
    13921 tgacgatggc agagaagtcg tactctccgg ccataccgtc gctgaacgtc gcccgcagcc
    13981 gatgtccacc gagacacttt attttcgcaa ctttaatcat cgtcgaggcc cgcgatcttg
    14041 atgagcggcg tattggtccg cgcccagctc caattatcct ccagtgcgta gggcggattt
    14101 tagccgaagg cgtgacccgc cgtcttgtcg cggacaatgg cggattacgc cttcggctcg
    14161 atccgcccta cgagctttaa gcaacagatt tagcccgaag ctttgccgaa aagtcgcgaa
    14221 gggcttttgg aacaaagtcg acaaatacca ttgccgaaac gtatgagcgg aatatctctt
    14281 cggccgattt ttctagaaaa aagctgccgt aatcctgatg aacgagccta tttcgttcgc
    14341 gtccaatttc tataaaatct gaaatcgact tcgccatgtt ctggtctagc ttgacaattt
    14401 cggccatgtg caatcggaag tcatcgccaa acatattgaa aaacgcgttt gcattgttag
    14461 ccttccactc gaaccaagtg tgatattggc gactgaccgc tttgtttttt acgagggtag
    14521 gtatcattga gttacctccc gccacctcgg cgcaaaaaga cacgacgtct gacgtaatac
    14581 ggaactcgaa atggcttgct gcacatagaa gcaacgtctt ccggaaattt tcattaagag
    14641 tattccgaaa cgaaacggcg ttgtactgat ccatcatgct gactattgcg gaatattcgc
    14701 catagagctt gtcaacaatc gttcccatct gagcgcccgc cctataaagt actaattagg
    14761 gctcttgcac gctttaacct cgtcccgaca tttgtggtac gagttgtacc ctcaagtgaa
    14821 gcacttacaa actgacgatc tcgttcaagc gcagaaactt catcggggtc tatttttcct
    14881 tttagtagcc gcctcttttt aaaggcttcg tcgcaggtcg cggtaaaaac cgcttcgaat
    14941 aaggcaatgt taaaccgatt gtttttcgga ttcacgaacg catcgtcagg caaatcctcg
    15001 caagcctcga gaaaggaagt taggagcttc tgcaagtact cattttgctt gctgtcgtga
    15061 ctctcgcaca ttcgagaaaa ttgattgagg aatcgtacca tagatggcgc gtattcatgg
    15121 ccactaatta gcatcgcgag tcctcggaga agaatttcaa tatccttcat atgaagatcc
    15181 ggctctggcg aattcaaaat agtccgccag cgactgtcgg tatttacgcg atagagcatg
    15241 tcataaaatt tggaatgata catgctcgtt cgaatttctt gaggacgcag atttattccg
    15301 cccgtgttta aacggttgaa cacctcatac atcgaggaat cgtcccccgc cggactgttc
    15361 tgcttaacga taatatttcg gatcggtcgc aaatcgaatt gcgtcttgta gtcgcctagc
    15421 gtcgaataat tcaatccatg aaatttatta gtttgatttg gcaatttttc tgagagcttg
    15481 agtttgaagt catcaaaaaa acgtcatcgt gcaacacttc atcaggaatt cttccctccc
    15541 tgtcgaaaat cgcacgaatt tcgacacgct tatcttttcg tggaaatttg cgcttgatga
    15601 aatagtagat cgacatgagt cgttgttgac cgtcgatcac gaggaagcga ttgcgtgcct
    15661 gctcgtacaa gaatagctga ggcacaggaa ttcccagaat caacgattca attagtttcg
    15721 atgcccggac ttggtcccaa acaaagttgc gctgaaaacc aggaatacga acagcaccag
    15781 actctatgaa attaaatagg gttgacacgt tgaaatcatt aggcgttgcc gtaatgtcat
    15841 attcgtcaat ttgcaactca tcttcttcgt cggcatagtc gtcgaaccat tcagccattg
    15901 cgaacccacc gtcccagttt tgttggtgca actatagcgc gaattgtcca actcgaacaa
    15961 taagcaaaaa cggcggcctt tcgcccgccg ctctgtttcg atggatgccc gggtcaagcc
    16021 cgggcatgac gcatgaatca gctcgtaggc gaattagcga aagcgtaatc cgccgttacc
    16081 cccgtcgaga gggcggatta cgccttcgcc tcaatccgcc ctgcgagcta taggtatgga
    16141 tggtggcgcg cgcgggagca tgccggaatg gtgctggcga atgcccgaag ctagtagaaa
    16201 atggaatcgg cccggggtag gtttcagcgg agagctgaaa catgaacata cgacgcgggc
    16261 tatggcgcac ctggatattc ctgacggcgc tgtgggtcat cgggacggtg acgctggcct
    16321 atttcgtcct gctcgagagt gtggccagcc ggaagtacca gtacgtctac aacatgcgga
    16381 gcgacgtgcc cgatccgaat aaggtggatt ggaccaagga tttctattcc ctgatgcagt
    16441 ccccctcgaa ggaaaagcta gcctcgacct tcgatatcca gacgtaccag tacgcgacga
    16501 cctgggacga ggacgtgaaa aaaggcatgc tgataacagt agaatttcca gataaatcgc
    16561 acctctacat gagtgcccaa atgacccagg tggaccgaga ttacgtcgcc aaggcgttct
    16621 ggaaccaaag gtgggaacgg tggggtatcg aaacgttgcc atgggtagcg ggcgccatcg
    16681 tcccgccgat catcctgctt ttgctcggta gctttatggt gtgggtcggc cgcggtttca
    16741 cccgcgactg aacttatccc cacttggccc gcatcgctcg caattccgcc gtgtcatgat
    16801 atcgtaaccc ctatgcaatc tacccaatgc acacgtagag cgggcggcat gcaaccagat
    16861 tcggcagcgc taacacttcc accgcctctt tgaaagatgc gtaacggcaa gctctcccgc
    16921 gatcagcaca acgagagcgc cgaacgtgag ggggtcgtgg atgttgtaga tgattgcagc
    16981 ccagccgatg gttttcatgc gctcgattcc ccaactcctg agagctgctt ttcagtttgg
    17041 gaatcgcctt gcccggtctc ggcccgtcgg gcagcgcccg tcacggcgcc ggagatagtg
    17101 tcgcctttta gcgtccggca ggacggggca tcaccccata gtcttttcga taacagacac
    17161 agtcgcctaa tcggccacgt gggctcgtgc cgcagcacta tttctggagt atgggacgcc
    17221 ggcacgattt caggacctca aaacgtcggg cgcgcgcgct gtaccggata atccgcagtc
    17281 gcgtagctgt atccgagggc ggtccgctgg tccggaaggc gctccaaagc gagcagcgcc
    17341 aactccacca tccggggaag cttctcggtc gacttctccc acgtgataat tgtctgacga
    17401 gacccaagcc ccagcgccat acggagcgcg tcctgggtga agtggttacg cttgcgccat
    17461 tcagggagtt cagtgccgat catcgggcac ctccaatgtc cagaaactgg acgtccaatg
    17521 gttggacatt ttgttgtgat ttggtcattc ggggccttaa aagtgcaatc aaaacggggg
    17581 tcggcaccct gaacatacgc agagaaagtt aaataatagc taaacgtctc gataagcaat
    17641 tttggccgaa gctcatgtga gggctatcgg acagctttgc tgtccgactg cctgttcgtg
    17701 agacggatgg attgagcgag cgacggcgtc gaacggctga cggtcccgcg cagacgcagc
    17761 gcgatcgcat ataactgagc gcagccatga ggaaggcatc gtcccagcga gcgcgtttga
    17821 ttttgcgccg tatggatgtg cgatcggggt gggctcgctt aggcgaggtc gtcccgcgca
    17881 agcccgccca tcgcctcaat ctcccggcgc agccactccg ggtccatatc aaaactgttc
    17941 ggccaggtcg gcgcaccatc ctccagaaat acgcgcgcga aatatgcggg gtcgcgcaag
    18001 ggctcgccca tcgggccggc ctcggcgacc agcactgaga aatcgcgctc gcccgccgtt
    18061 ccgtcgctga acgtcgcgtg caggcgatag ccgccgagat gtttgatttt ggtgacctta
    18121 atcatcgtcg agttctgcgg gagagggagc aggtatccac gtctttacca cgcggacgcc
    18181 caaaaactag atggccggga caaagcccgg ccatgacgaa gagagagaaa ctgggtcccg
    18241 gttctgcggc gcaccacttc gtgctgcgcc gcgcccggga cacgagatta attatcgagg
    18301 aagctccgca gcttccttga cctgctcgga tgcttcagct tgcgcagcgc cttggcttcg
    18361 atctgcctaa tacgctctct tgtcaccgag aactgctggc cgacttcttc cagggtgtgg
    18421 tcggtgttca tgccgatgcc gaagcgcatg cggagcacgc gttcttcgcg cggggttagc
    18481 gacgccaaaa cccgcgtcgt ggtctcgcga aggttgctct ggatcgcggc gtcgatgggc
    18541 aggatcgcgt tcttgtcctc gatgaaatcg ccgagatgcg agtcttcctc gtcgcccact
    18601 ggagtctcca gcgacagcgg ctctttggcg atcttgagaa ccttgcgaac cttctcaagc
    18661 ggcatgccga gcttttcggc caattcctca ggcgtcggct cgcggccgat ctcgttgagc
    18721 atctggcgcg aggtgcgcac gatcttgttg atggtctcga tcatgtgcac gggaatgcgg
    18781 atggtgcgcg cctgatcggc aatgcttcgc gtgatcgcct gccggatcca ccacgtcgca
    18841 taggtcgaga acttgtag
//
LOCUS       DASWGM010000509         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_731174,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000509 DASWGM010000000
VERSION     DASWGM010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731174"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VGA15_14690"
     CDS             <1..438
                     /locus_tag="VGA15_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HEX9588980.1"
                     /translation="TATARVDQSVTVQYGARANEAAIRSQLQTLAVFAAVTTSTTNPN
                     AAAQVSALSQRVAQNLATQPGQQTIEDIQSDFATAQMSMKNAASRQTQAKGMLQGIVD
                     QAESVSPDQVATEILALQTNLQASYQTTSMLSQLTLTKYLPVG"
     gene            527..1312
                     /locus_tag="VGA15_14695"
     CDS             527..1312
                     /locus_tag="VGA15_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588981.1"
                     /translation="MTDQIHLMVATPCFGGQVSSIYAGSVFHLQRAVRSKPSMDLTIL
                     MRDGDALITRARANLVTLFLDNPSATRLLFVDADIGFTPDQVFRLIESEADMVAGVYP
                     IKRVNWDKAKRMLESNRPASPAAALDYVLEIDDPDRVVTVDGFTRVRFAGTGFLMIRR
                     HVLERMCQHPAYASLRFFNEHSFDALAGSRNRFALFECMIDPKTGTYLSEDFAFCKRW
                     TDIGGEIWADLESRLDHVGPSVFHGDVSSQFAAPASIRAANAA"
     gene            1317..>2437
                     /locus_tag="VGA15_14700"
     CDS             1317..>2437
                     /locus_tag="VGA15_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588982.1"
                     /translation="MPTLNTTLRLNAAEDPIARDRSGSAAAMAELFGPLDRLLSGGGD
                     ARLAINPASGANGYGCQPFPCPETLSFASSTATSISERAYDRANDARQSLMQSAIAVG
                     IDAAFDARIEAMRDELKACLGLSETGVDVVFSPSGTDSQLQALLLAGALLGPVLTTII
                     VAADQTGSGTADTARGHHFSAATAHGYRVRKGEPVAGLTHSVASVALPSFDETGGFRP
                     HADGDARVLGAIERSVANGSNVLLQIMDSSKLGWRAPSDQCLDEIRARWPDRVQVVVD
                     ACQMRVGRPRLQSYLDRGYIVIVTGSKFFTGPAFSGALLVPAGPSKALGAVTEVASGF
                     LEYFSRSDWPRNWPALRSCFPIRANLGQWLRWEAALEEIR"
BASE COUNT          439 a          763 c          756 g          479 t
ORIGIN      
        1 acagcgacgg ctcgcgtcga tcagtcggtg accgttcagt acggggcgcg cgccaacgag
       61 gcagccattc gttcgcagct tcaaacgctg gcggtcttcg ccgcggtcac gacctcgacg
      121 acaaatccaa acgccgccgc gcaggtctcg gcactcagcc agcgggttgc gcaaaatctt
      181 gcgacgcaac caggacagca aaccatcgag gatatccagt cggattttgc gacggcgcag
      241 atgtcgatga aaaacgccgc gtccaggcaa acccaagcca agggcatgct gcagggcatc
      301 gtcgaccagg ccgagagcgt ttctcccgat caggtggcca ccgagatatt ggcgctccag
      361 accaacctgc aggcgtccta ccaaaccacc tccatgctgt ctcagctcac cctgaccaag
      421 taccttccgg tcggctaacc cgggtcggcc gttaggcttt cgttaactac gtctatttac
      481 tgtttggtga gaagtgcagg caatgtcagc ggctccggag ccattcatga ccgaccagat
      541 tcacctgatg gtggccacgc cctgcttcgg cgggcaggtc tccagtatct atgccggctc
      601 ggtcttccat ttgcagaggg ccgtgcgttc caagccgagc atggacctca cgattctgat
      661 gcgtgacggt gacgcgctga ttacccgtgc gcgagccaat ctggtgacgc tgttcctcga
      721 caacccctcg gcgacacgcc tcttgttcgt ggatgccgat atcggcttca cgcccgacca
      781 ggtattccgg ctgatcgaat ccgaagccga catggtggcc ggtgtctatc ccatcaagcg
      841 ggtcaactgg gacaaggcca agcggatgct ggagtccaac cggccggcat cgccggcggc
      901 cgcgctcgac tatgtgctgg agatcgacga tccggatcgc gtcgtcaccg tcgatgggtt
      961 tacccgggtg cgctttgccg gcaccggctt cctgatgatc aggcgtcacg tccttgaaag
     1021 gatgtgccaa catccggctt acgcgtcact gcggttcttc aacgaacatt ccttcgatgc
     1081 gctcgctgga agccgcaacc ggtttgcgct gttcgagtgc atgatcgatc cgaagaccgg
     1141 gacttatctc agcgaggatt ttgccttctg caaacgctgg accgatatcg gcggcgaaat
     1201 ctgggctgat ctcgagagcc gcctcgatca tgtcggcccg tcggtctttc acggcgatgt
     1261 ctcgtcgcag tttgcagcac ctgcatccat aagagcagca aacgccgcgt gaaatcatgc
     1321 cgacgttgaa caccacgctt cggttgaatg cggccgagga tccgatcgct cgggatcgat
     1381 ccggttcagc cgccgccatg gccgagctgt tcgggccgct ggaccgcttg ctcagcggcg
     1441 gcggcgatgc gcggctggcg atcaatccgg cgagcggggc caacggatat ggctgccagc
     1501 cgtttccttg tccggaaaca ttgagctttg cctcaagcac agcgacatcg atctcggagc
     1561 gggcttacga ccgtgccaat gatgcgcggc aatcgttgat gcagtccgcc attgccgtcg
     1621 gcatcgatgc cgcgttcgat gcccgcatcg aggcaatgcg cgacgaattg aaggcttgcc
     1681 tcggactgtc cgaaaccggg gtcgatgtcg tcttttctcc gtcgggcacc gactcgcagt
     1741 tgcaggcgct gttgctcgcg ggggcgctgc tcggccccgt gttgacgacg atcattgtgg
     1801 ccgccgatca aaccggaagc ggaaccgcgg ataccgcgcg cgggcatcat ttcagcgccg
     1861 cgaccgcgca cggatatcgg gttcgcaagg gagagcccgt tgccggcctg acgcattccg
     1921 tcgcaagcgt cgcgctgccg tcgttcgatg agaccggcgg cttccgcccg cacgccgacg
     1981 gcgatgcacg ggttctcggc gcgattgaaa ggtcggtcgc gaacggaagc aatgtgctgc
     2041 tgcaaatcat ggattcgtcg aagctgggct ggcgggcgcc aagcgaccaa tgcctcgacg
     2101 aaatcagggc gcgctggcct gatcgggtcc aggtcgtcgt cgatgcctgt cagatgcgcg
     2161 tcggccggcc gcggctgcaa agctatcttg accgcggata catcgtgatc gtcaccggct
     2221 cgaagttttt caccgggcct gccttcagcg gcgcgctgct ggtaccggcc ggtccctcga
     2281 aggcgctcgg tgcggtcacc gaggttgcat cggggttttt ggaatacttc agccgcagcg
     2341 actggccgag gaactggccg gccctgcgtt cgtgttttcc gatcagggcc aatctcggcc
     2401 aatggcttcg ctgggaagcc gcactggagg aaatccg
//
LOCUS       DASWGM010000510         2912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2670551,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000510 DASWGM010000000
VERSION     DASWGM010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2670551"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VGA15_14705"
     CDS             <1..1254
                     /locus_tag="VGA15_14705"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX9588983.1"
                     /translation="IEQQGRTLSARCGRGVRVVAVTARSKAKKRGLDLRGIDWAKDPL
                     TLASDPGIDCFVELMGGSGEPALSAIEAALKSGKSVVTANKALIAKHGLRLATSAEKC
                     GGALNFEAAVGAAIPIIKTLREGLAGTDVNRVYGILNGTCNYILTRMEQDGLSFAECL
                     KDAQRLGYAEADPSFDVDGHDTAQKLAILASLAFGTRVAQSAVYVEGISSIAPEDLRA
                     AEELGFRVKLLGVAVRTAKGIEQRVHPTMVPKSSSIAQVMGVTNAVTIDGEGIPPITL
                     VGPGAGGAATASAVIADIADVVRGIRAKPFGRPVAQLRETKKAPMERHEGGYYIRLMA
                     RDLAGTAATIATRLAEQKISIESIVQRHPDGVDVNGAARKASPVPVILITYATSEDAV
                     YRALRAVQRDKVISGRPQVIRIEKN"
     gene            1337..2338
                     /gene="glpX"
                     /locus_tag="VGA15_14710"
     CDS             1337..2338
                     /gene="glpX"
                     /locus_tag="VGA15_14710"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX9588984.1"
                     /translation="MSTHISVPPQLLLERILTLEIVRVTERAAVSAARLRGHGNEKAA
                     DQAAVDAMRRELNKLPIEGTIVIGEGERDEAPMLFIGEKVGLNAGPKVDIAVDPLEGT
                     TLCAKNMPGSIATMAMADGGTLLHAPDVYMQKIAVGPGYTKGVVELDASPADNVRRLA
                     KAKGVDASAITVLVLDRPRHSDIIAGVRSTGAAVRLITDGDVAGVIHCADPDNTGVDM
                     YIGTGGAPEGVLAAAALRCIGGQMQCRLILDTEEKRERAHKMGVDDPRFIYGIEDMAK
                     GDCLFAATGVTTGSLLSGVKFRKDGVIETETVVMRSVTGTVRYIKAEHRQLEKFHLD"
     gene            2351..2605
                     /locus_tag="VGA15_14715"
     CDS             2351..2605
                     /locus_tag="VGA15_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588985.1"
                     /translation="MAANVVIRPVGRDERAAWEPLWNGYLAFYEATLAPGASDVAWER
                     FHDSDEPMFLLGAYVDGRLRLPEPGLKRIWPVRAVTSQFV"
BASE COUNT          554 a          872 c          979 g          507 t
ORIGIN      
        1 atcgagcagc aaggccggac tttgtcggcg cgttgtggac gcggtgtgcg cgttgtcgcg
       61 gtcaccgcgc gttcgaaggc aaaaaagcgc ggtctcgatc tgcgcggcat cgactgggcc
      121 aaggatccgt tgacgctggc cagcgatccc gggatcgatt gctttgtcga attgatgggc
      181 gggtcgggcg agcccgcgct atcggcgatc gaggcggcgc tgaaatccgg caaatcggtg
      241 gtcacggcca acaaggcgct gattgccaaa catggcttgc ggctggcgac gtcggccgag
      301 aaatgcggcg gcgcgctgaa tttcgaggcg gcggtcggcg ctgcaatccc catcatcaaa
      361 accctgcgcg agggccttgc gggtaccgac gtcaatcgcg tctacggcat tctcaacggc
      421 acctgcaatt acatcctgac ccggatggag caggacggat tgtcgttcgc cgaatgcctc
      481 aaggatgccc agcgcctcgg ctacgccgag gccgatccgt cgttcgacgt cgacggccac
      541 gacaccgcgc agaagctcgc cattctcgcc agcctcgcat tcggcaccag ggtcgcgcag
      601 agcgcggtct atgtcgaagg catttcctcg atcgcgccgg aagatctgcg tgccgccgaa
      661 gagctcggat ttcgcgtaaa actgctcggg gtcgcggtgc gcaccgcgaa aggcatcgaa
      721 cagcgcgtgc acccaaccat ggtgccgaaa tcatcttcga ttgcacaagt gatgggcgtc
      781 accaatgcgg tgaccatcga cggcgagggt attccgccga tcacactggt cggcccgggc
      841 gcaggtggag cggcgaccgc atcggccgtc attgccgata tcgccgatgt cgtgcgcggg
      901 attcgcgcca aaccgttcgg acgtccggtg gcgcagttgc gcgagaccaa aaaggcgccg
      961 atggagcgcc atgaaggtgg ctattatatc cgcttgatgg cgcgcgatct cgccggtact
     1021 gctgcgacca tcgccacccg gctcgcggaa caaaaaattt ccatcgaatc aatcgtgcag
     1081 cgccatcccg acggtgttga cgtgaacggc gcggcgagga aagcttcgcc ggtgccggtc
     1141 atcctgatca cttacgcgac cagcgaggac gcggtctatc gcgcgctgcg ggcggtgcaa
     1201 cgcgacaagg taatcagtgg ccggccgcag gtgatacgga tcgaaaaaaa ctaacgcgtg
     1261 gcccgaacgg accaggtgcg aaacgattgg tggcgggccg aaagacccgc ggtgaagttt
     1321 tagaggagtt ttagcgatgt cgacccatat ttccgttccc ccgcagctgt tactggagcg
     1381 cattctgacg cttgaaatcg tgcgggtcac ggaacgcgcg gcggtgtcgg ccgcgcggct
     1441 gcgcggccac ggcaatgaga aggcggccga ccaggccgcc gtcgacgcga tgcgccgcga
     1501 gctcaacaag ctgccgatcg aaggtacgat cgtgatcggc gagggcgaac gcgacgaggc
     1561 gccgatgctg ttcatcggcg agaaagtcgg gttgaacgcc ggcccgaagg tcgatatcgc
     1621 cgtcgacccg ctcgagggca ccacgctgtg cgccaagaac atgccgggtt cgatcgcaac
     1681 catggcgatg gcggatggcg gcacgttgct ccatgcgccc gacgtttata tgcagaagat
     1741 cgccgtcggc cccggctaca cgaaaggcgt cgtcgagctg gatgcgtcgc cggccgacaa
     1801 cgttcgccga ctggccaagg cgaagggtgt cgatgcctcc gcgatcacgg tgctggtgct
     1861 cgatcgcccg cgccattccg acatcattgc cggcgtgcgc tccacgggtg ccgcggtacg
     1921 cctgatcacc gacggtgacg tggcgggcgt gatccactgc gccgatcccg ataataccgg
     1981 ggtcgatatg tacatcggta ccggtggcgc gcccgaaggc gtgctggcgg cggcggcgct
     2041 gcgctgcatc ggcggccaga tgcaatgccg gctgattctc gacaccgagg agaaacgcga
     2101 gcgcgcccac aagatgggcg tcgacgatcc gcgctttatc tacggcatcg aggacatggc
     2161 gaagggcgac tgcctgttcg cggcgaccgg cgtcaccacc ggttcgctgc tgtcgggtgt
     2221 caaattccgc aaggacggcg tcatcgagac cgagaccgtg gtgatgcgtt cggtcaccgg
     2281 caccgtccgc tacatcaagg ccgagcaccg gcagttggaa aaattccacc tcgattgaga
     2341 agagcgtgcg atggctgcca acgtcgtcat ccggcccgtc ggcagggacg agcgcgccgc
     2401 gtgggagccg ttgtggaacg gctatctggc cttctacgag gcgacgcttg cgcccggcgc
     2461 cagcgacgtc gcctgggaga ggtttcacga ttccgacgag ccgatgttcc tgttgggggc
     2521 ctacgtcgac ggcaggctca gacttccgga accggggtta aagcggatat ggcctgttcg
     2581 ggcggttact tctcagtttg tgtgaaggtt tgcggactcg gcgcgatgtc tgacttgagt
     2641 ccgtaatgct ctgcaaagcg gacatcggcg agagaaacgt agcggcctgc acaaggaggt
     2701 aacgctcaat cgcgggcgcc cgatgtcgag cgcgagtgag accgcgtaag cgtcacgcga
     2761 aggggccgct ggaagccaga cattcggaac ggaccgcgcc gtcatataca cagaaaagtt
     2821 cccgttttcg gtcgggtcct gtcggtggcc gggtactggc cctgaccctt agtggcctaa
     2881 tttgaaaatt agcagtggcc tcgtttgaac aa
//
LOCUS       DASWGM010000511        26454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1723582,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000511 DASWGM010000000
VERSION     DASWGM010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 26454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26454
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723582"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="VGA15_14720"
     CDS             <1..1114
                     /locus_tag="VGA15_14720"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX9588986.1"
                     /translation="KTRNSPGYTVQVPVVDIVEVGAGGGSIAWLDPSGRLRVGPQSAG
                     STPGPACYGRGGVDPTVTDAKLTLGILDPLTFAEGRMQLDVESARSAIARVSLPMKLS
                     VEEAALAIVKVAEECMTNALKLITIQRGHDPRDLALIVSGGAGPLLAASLGRELNVKR
                     TVIPTHPGIFSAWGMLAARPRADLRRTVLKTVCEEEMNSISQLFAELEQEANEYFVGT
                     SAIKLKFQHRAEIRYRGQEHSVSVGFSGGSAEELLSDFHATHQKTFSFALKATAEITM
                     LHLQTEVLSEVIGLPKIQGNGEDTLDSALKGQRSVFLTKEKGWVACNVYDRNRLPIGS
                     SIPGPALVEEPTTTTFVLSDQRFARDETGQLIMWSR"
     gene            1768..1923
                     /locus_tag="VGA15_14725"
     CDS             1768..1923
                     /locus_tag="VGA15_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588987.1"
                     /translation="MALPDNGLFGAIADKKSFRQLKLHRLATANDRPRVAEPRALPMP
                     VPEEIST"
     gene            1956..2591
                     /locus_tag="VGA15_14730"
     CDS             1956..2591
                     /locus_tag="VGA15_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9588988.1"
                     /translation="MAATDQTRAAAAPRIKLLSHGFSIDHPDPEHGEQLMSDALGVAD
                     RDAMHGILRQLVKASVNGQEPDAVNLSFMISMVKSIKPRDSVEAMLVAQMVSVHVMAM
                     RCAHHLANAEDIAQQDSAGRALGRLARTFPAQIEALSRYRNNGEPAITVQNVSVQDGG
                     KAIVGNVTQHASVIVSDKSPAPAVTDARMVSMPDRGAKEHDPVDAKREAKA"
     gene            2588..2854
                     /locus_tag="VGA15_14735"
     CDS             2588..2854
                     /locus_tag="VGA15_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGGxSTG domain-containing protein"
                     /protein_id="HEX9588989.1"
                     /translation="MSDHARNTEPMLASPRCGAKIRSGGSCRSPAVRGKRRCRMHGGA
                     PGSGAPRGNQNARRHGLFTKVAIAERKQIQAMLGEARKLLQAMK"
     gene            2833..2929
                     /locus_tag="VGA15_14740"
     tRNA            2833..2929
                     /locus_tag="VGA15_14740"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2889..2891,aa:Ile,seq:gat)
     gene            2902..3531
                     /locus_tag="VGA15_14745"
     CDS             2902..3531
                     /locus_tag="VGA15_14745"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9588990.1"
                     /translation="MQVRVLPGTCVRQKADLPTREERVFLFLDLVGSTSLAESMGELR
                     MHDLLTRFFFDIDEAILAHGGEVHAYVGDEVIATWRLAANMSERRYLDCFFAIQDRIA
                     EKADLYRGEFGLVPTFRAGLHAGSVVISECGDSHRQIAYFGDTMNVAARLQERCKEAG
                     RALLVSAELLRLVRPSRDLVVEALGPTQLRGRAAAVEVFAVERRRLANE"
     gene            complement(3598..4971)
                     /locus_tag="VGA15_14750"
     CDS             complement(3598..4971)
                     /locus_tag="VGA15_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9588991.1"
                     /translation="MKTIAIFGSYVLSRKDGAQEVLRDHWVLLEGRRIAAVTRDRPSA
                     TEIFDKPGRFVLPGLLNLHNHCFSEAVARTHTEDGNGRRNNQSIVYTVLLPLTKRGAD
                     LLSPSERLAIARLGILQLLKGGATTVMEPFRNTIPEMFDAADEMGIRFYGAPYLFSTS
                     DAKAGPDGVVRYAGDDGQVDLDTWNALYHRWNGRGDGRIGLAMSPHATDTCGPDLLRA
                     CAARARELDVPITTHLAQSHAEVVTIGKRYGNRTPAEYLDWLGLVAPDLLAAHCIAST
                     DGDLKLMAARGATVLNCPRVFARSGVAAAFSRFAEHGVRTVVGTDGYNMDLLGELNAA
                     SMISKIASGRAEVASAPDLIESVTATAAALIKRPDLGMIAPGATADLTVVDLTHPHLQ
                     PLFDPRRGMVALGNRANIDQVVVDGRVLIDAGHYVHGDEAAIISAATAAIGKIWDLPE
                     AQAAFNG"
     gene            complement(4968..6551)
                     /locus_tag="VGA15_14755"
     CDS             complement(4968..6551)
                     /locus_tag="VGA15_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588992.1"
                     /translation="MLVSRRSLLRAAVAAPAFSLPRILRAQSQSTLRFIPVIDLTFVD
                     PIYSTAQVSRNHGFMVYDTLYGMNSGLEVSPQMVSGHVVSSDDRQWDLTLRDGLLWHD
                     GERVLARDCVASIRRWATRDGFGAELMAATDELSAPDDRTIRFRLNRPFPLLPAALGK
                     SAIQAPFMMPERLASQDAFKPLTEVVGSGPFRFVADERVPGARNVYARFDRYQPRQDG
                     KPDWTAGPKIVHYDRVVWTTMPDAATGVAALQTREQDWQETTPHDLLPVLKRAGGITT
                     RILDPRGYTCMLRVNHLQPPFDNPAIRRALLGAIDQSAFMTAVAGDDPAYQSSPIGFF
                     APGTPMASDVGLNVFRGPRDMAKVRADLKAAGYNGEKVVLLVPTNSLAQKPLGDVAAD
                     MLQQAGMNVEYAGLDFGVVLQRQLKKDPIAQGGWSAGVGNWQGIDWLNPAGNSNLRGD
                     SKAPGWYKSEKMGALRSQWLVASNVADQQRICREIQALSFEEVPYFPIGQYKQPTAYR
                     SSITGILDGTAVFWNVKPT"
     gene            complement(6624..7508)
                     /locus_tag="VGA15_14760"
     CDS             complement(6624..7508)
                     /locus_tag="VGA15_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX9588993.1"
                     /translation="MQDSKALASIPLIPANTAPPAPEYPWPAPYTSAMFLSFDVDAES
                     AWTSKDAAHAQRLITMSYGGYEARVGTPKLLELLDQLDLKATFFITGWSADAHPAMAE
                     AILKAGHEIGHHGYHHLLPDPGDPWIEEELERGFEALKRRLSVKPTGYRAPYGEFTEE
                     LRVALVRHGIVYTSSFRDDVRPYRHRLADGRPGTIELPVTASYDDWMHGLSARFSPRS
                     IFPKEHVLSIWKDELDEVRDWGAMVTTVLHPQCSGRPMRLRLLREFLTYAKSCPDLWI
                     ATGEAIAANFQSHEAATP"
     gene            complement(7495..8310)
                     /locus_tag="VGA15_14765"
     CDS             complement(7495..8310)
                     /locus_tag="VGA15_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588994.1"
                     /translation="MMKRFLAAAFLCAATGSAIAVELPPEIEKQGSIRAAIVPNYPPL
                     EFRDPATNTLTGFDVELGEALGRKLGVKIVWQETSFDQMMPAIATGRVDAILSGMTDL
                     ASRQDTATFVDYLRNGPRFFAQQSRAAEFKDPMALCGKKVGASRRTSFPKLVAAWSDA
                     HCGGDPIIFVGTEGSADARTQLKQGRLDAAVQGGETLPYMMDLEPGVYIPIGDVFALQ
                     FTGLALSVKEKALQQAVVEALDALIADGSYRVLLAKWKLADYGVKKATINAGQ"
     gene            complement(8324..9088)
                     /locus_tag="VGA15_14770"
     CDS             complement(8324..9088)
                     /locus_tag="VGA15_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX9588995.1"
                     /translation="MTRPLVAIRSVSKNFGELQALKRVSLDVRAGEVLCLIGASGSGK
                     TTLLRCINQLTSIDSGGIWLDGELLGLREEGGRLHRLTPQQIARQRLKTGMVFQRFNL
                     FPHKTALENITEGPVQVQGRKREDARGEAMELLRRVGLAAKADAYPAQLSGGQQQRVA
                     IARSLAMKPMLMLFDEPTSALDPELVGEVLTVMKELARSGMTMMVVTHELGFAREVAD
                     RVVYMDHGAIVESGPAADVLGAPREARTQSFLSAAI"
     gene            complement(9085..9942)
                     /locus_tag="VGA15_14775"
     CDS             complement(9085..9942)
                     /locus_tag="VGA15_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9588996.1"
                     /translation="MRAADTLAGGFPDISGMTLARQAHWRRWLAAAAILVVLVAIGRA
                     FANGQIEWAYVGRFLTAKVILEGIVNTMVMAVLAMALGIVLGVVVAIMRLSPNPVLKS
                     VAAGYTWLFRGTPLILQLLLWFNIALVFPTIGIPGLWSARAVDVMTPFLSALLGLGIN
                     QGAYTSEVMRAGMLSVDAGQYEAAQAIGMGRLHALRRIILPQAMRVVIPPLGNEFIGM
                     VKATSLASVIQYPEVLHNAENIYYANSRVIELLIVAGFWYLLVVSILTPSQMLLERRF
                     ARGSLQIAR"
     gene            complement(9939..10763)
                     /locus_tag="VGA15_14780"
     CDS             complement(9939..10763)
                     /locus_tag="VGA15_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9588997.1"
                     /translation="MTRLSFSVGFAALLAFTIAQAAELPESIKQAGVLRLTVNSTYAP
                     MEYRDPATNELVGLDIDLANEIAKRLGVKIVWSETPFAELIPSLQTRRADFIISGISD
                     RSSRRETADFVDYLTTGPQIFVLTENAAKSAIELCGKRVGTTRSTSFPIEIEKWSRQN
                     CEAGGKPAVQYVPGENSIDVRNQLKQGRIDAAVQGSETLPYAQQQEAGKYRVVGEPFS
                     TGYQGIMFRKDDAALREVVTQHLAAMIADGSYKAILDKYGLSANAVPQPMTNAATQ"
     gene            complement(10765..11760)
                     /locus_tag="VGA15_14785"
     CDS             complement(10765..11760)
                     /locus_tag="VGA15_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HEX9588998.1"
                     /translation="MLVRVLRAAAAQMGPTQNADSREHTLGRMLELLDAAAARGASLV
                     VFPELAFTTFFPRWLVEGKALDHHYERSMPNPAVQRLFDRARALRIGFYVGYAELTSD
                     RRRFNCSILVDGDGEILGRYRKVHLPGSVEPRAGARYQQLEKRYFEYGDLGFPAFRTG
                     PDWRHAIMGMMICNDRRWPESWRVLGLQGVELVCVGYNSAAYDPNGGVTEDAALRTFH
                     SKLVTQANAYMNATWAIAVAKAGNEDGSGLIGGSCIVDPNGRIVAETQTLADEVVVAD
                     LDLDFCRQGKDKMFNFAAHRRPEQYTVITERAGVIEPAALDANQYDAATRAGRRS"
     gene            11910..12809
                     /locus_tag="VGA15_14790"
     CDS             11910..12809
                     /locus_tag="VGA15_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9588999.1"
                     /translation="MNLRQLEILRAVIRHRTTVAAADELALSQPAISNALKAMEAQAG
                     FALFERVNNRLFPTAEAMALYKESEAIFALHAKLENRVRDLRECRSGHLSIVATPPLA
                     YSIIPPALSGFLRRRQQTRVFFDVRRYEGIIDGVLSRVAELGFALGLSHHPGIAHEVV
                     HTGEMVCVLPPKHPLAERPIISASDLVGLPFIGLERGTRLGEAVRESFAQAGAPFQPT
                     VEVRYCNTACVLAAAGVGAAVVDPFSPRQGGSHDLIVRPFAPKTVAVAYMRWSEAEPL
                     SRLAKTFLNEVRQASGLLGRDGC"
     gene            complement(13023..13274)
                     /locus_tag="VGA15_14795"
     CDS             complement(13023..13274)
                     /locus_tag="VGA15_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589000.1"
                     /translation="MPLLTPVTMGGEWAEFNPWQIPIGSATPGAFELMGVTVLRIQSP
                     DEAAEVVSAGASLAFDGDRQVAVLIWQRMLGKKKWVEPR"
     gene            complement(13585..15036)
                     /gene="glgA"
                     /locus_tag="VGA15_14800"
     CDS             complement(13585..15036)
                     /gene="glgA"
                     /locus_tag="VGA15_14800"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX9589001.1"
                     /translation="MKPLRVLSVASEIYPLIKTGGLADVTGALPKALKSEGIEARTVV
                     PGYPDVLNELESADEVLRFPNFYGGPARVLSCSMAELDLFILDAPHLFARPGNPYVAP
                     DGADWPDNAIRFAALGRTAAEIGEGAIPAFVPHVVHAHDWQAGLTPAFLHYSERPRPG
                     TIMTVHNLAYQGKFPREMLRDIGLPPESFTIDGVEYYGGIGFLKAGLQFADRITTVSP
                     TYAVEIQEPDAGMGLDGLLRARSDVLSGILNGIDIAVWNPGTDPRIASPFGVNTLADR
                     AANKTALQRRFDLPLAPDAFLLGVISRLSWQKGLDLLLESLPLALCKGVQLVLLGNGD
                     RDLEARYIAATKSYPDQIGVLIGYDENLAHLIQAGADALVVPSRFEPCGLTQLCALRY
                     GAIPIVSRVGGLADTVIDANEMTIPAGVATGIQFGPVTSENLTGAVRRAKGLFHNKPA
                     WQRIQKNGMTVDVAWHNRARHYADLYRQIAKPD"
     gene            complement(15033..16295)
                     /gene="glgC"
                     /locus_tag="VGA15_14805"
     CDS             complement(15033..16295)
                     /gene="glgC"
                     /locus_tag="VGA15_14805"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX9589002.1"
                     /translation="MRPAGNEPLARHAIAFVLAGGRGSRLLELTDRRAKPAVYFGGKS
                     RIIDFALSNAMNSGIRRIAVATQYKAHSLIRHLQMGWNFFRPERNESFDILPASQRIS
                     EKNWYLGTADAVYQNIDIIEPHNAKFIVLLAGDHIYKMDYELMLQQHVEQGADVTIGC
                     QEVPRSEASGFGIMHIDDTDVIKSFLEKPTDPPAMPGKPDKSLASMGIYVFNSEFLFE
                     ELRRDAEDPNSSHDFGKDIIPYIVKHGRAVAHHFSRSCVRSHNDARSYWRDVGTLDAY
                     WASNIDLTDILPELDLYDRSWPIWTHAEITPPAKFVHDRGGRRGEAVTSLVSGGCIIS
                     GAAIRRSLLFTGVHVHSFAKIENAVILPYVEAARGSRLKNVVIDRGVRIPKGLVVGED
                     PECDAKRFRRTEQGICLITQPMIDRLSA"
     gene            complement(16654..18150)
                     /locus_tag="VGA15_14810"
     CDS             complement(16654..18150)
                     /locus_tag="VGA15_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589003.1"
                     /translation="MLKKLVLLAAAAMTALCLLPQSLSAQGKKDSVVMAMTLEPPGLD
                     PTSGAAAAIAEVTLYNVYETLTKVREDGSVVPLLAESFETAPDLKTYTFKLRKGVTFQ
                     NGEPFNAEAVKFSYERAAASTSTNKDKSLFQSIAQITTPGGDTVVINMKNPEPNLPFL
                     LGQASASIVEPKSAPTNATAPVGTGPYVLAAWAKGSSITLTKWPGYRNAAAIHLTKIL
                     IRFISDPSAQVASLLSGDVDAFPRVAAARSLAQFKADPRFAVQIGGSRAKTIVAINNK
                     KKPLDDVRVRRAILAAIDRKAMIEGAADGFGVPIGSFYTPGSLGYIDTTDINPFDVEK
                     AKKLLAEAGVTTPLEVSLKLPPPAYARQGGEVLAAQLAKIGITAKIENVEWAQWLSSV
                     FNGPHNYDLTIVSHVEPFDLVKYTEDNYYIGYKSEAFNALYKSITEAANDADRAKLLG
                     DAQHMLATDAVAGFLYQPQLITIANKKLKGVWGQVPQFANDFSDWAWE"
     gene            complement(18215..19246)
                     /locus_tag="VGA15_14815"
     CDS             complement(18215..19246)
                     /locus_tag="VGA15_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9589004.1"
                     /translation="MSTTTTAGVAPEPMLLEVDDLVQRYTLPRENLFQAAAQVLALNG
                     VSVQLAAGKSLGVVGESGSGKSTLARAVMALETPTSGSVKLLGRDLHRLPAGELRRAR
                     RDFQMVFQDPYGSLDPRQTVARIVAEPLTALGHIDRAEQRARVAAVLKQVGLRDADMD
                     KFPHEFSGGQRQRIAVARALITQPKLIVADEPVSALDVSVQAQVLNLLEDLQDEFGLS
                     YVLISHDLAVVEYLCDEIAVIYLGRIVEQGRPQDLFSRCAHPYTRALLEAVPRIRPGR
                     ERRRRGAQAIASQSVGPTGCAYAGRCSFADAHCRAVAPMARSVGPDHTSACHHAETIM
                     ALPPLAVEV"
     gene            complement(19243..20250)
                     /locus_tag="VGA15_14820"
     CDS             complement(19243..20250)
                     /locus_tag="VGA15_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589005.1"
                     /translation="MTDRKPLIEVDNLRVRLNTSRGPADAVRGVSFSLARGETLGLIG
                     ESGCGKSVTAMALMGLLPESAVVSGSVRLDGQELVGLDEADYCKLRGNRVSMIFQEPM
                     TALNPMHTVGRQVADPLRRHRGFSRAQAYREAIALLDRVGLPDAAKRAAAYPHQFSGG
                     QRQRITIAMALACEPDLLIADEPTTALDVTIQGQILDLIADLMAERGMSMILISHDLG
                     VIAENVQRMMVMYGGTVVENGTIEALLSGMGHPYSQGLLRARPRLGAPKGTRLQTIPG
                     TVPELADLPSGCAFADRCDIAEERCRAIFPPPVTVNAGHTVRCLRTDVSMANRIGAVT
                     A"
     gene            complement(20250..21170)
                     /locus_tag="VGA15_14825"
     CDS             complement(20250..21170)
                     /locus_tag="VGA15_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589006.1"
                     /translation="MSGIQPAAATPSNAAPPDAAALSQSRRSGNSFWQRARRHRSFVA
                     GGVLTMLMISAALLSLVWTPWPAYEIDMAHKLNPPSALHWLGTDTLGRDIVSLLLVGA
                     RSSIVVGIIAVGIGLLFGASLGLVAAARRGWVEEAIMRLSDFTFAFPALLSAIMLAAV
                     AGPGMVTSITAIGIFQIPIFVRITRGSANAIWAREFVLAARASGKGKFRITLEHVLPN
                     ILSILIVQATIQFALAILAEAALSYLGLGTQPPQPSWGRMLNDAQSLLFQSPSLAVYP
                     GLAIATAVLGLNLLGDGLRDLFDPRLSRER"
     gene            complement(21167..22117)
                     /locus_tag="VGA15_14830"
     CDS             complement(21167..22117)
                     /locus_tag="VGA15_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589007.1"
                     /translation="MSVFIVRRLLTLVATLAGASLIIFLVLDALPGNAAEMLMGADSS
                     PEAIRAMTVKLGLDQPLVVRYLHWIVGLLVGELGNSYSYGTPVADLIAERLTLTIPLA
                     VMAMLLTVVLALGAGVYTAANHNKIGDIGVMAATQFGIAIPNFWFAILLILLFSVKLQ
                     WLSAGGFVGWGEGILPGIRSLLLPAISLAVVQAAILARVTRSAVLDVLREDFVRTARA
                     KGLSRRQVLWGHVLRNAMIPVMTLMGLQFANLLAGAIVIENVFYLPGLGRLIFQSISN
                     RDLIVIRNCVMLLATMVVIVNFVVDVLYAAIDPRIKASEL"
     gene            complement(22252..23700)
                     /locus_tag="VGA15_14835"
     CDS             complement(22252..23700)
                     /locus_tag="VGA15_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9589008.1"
                     /translation="MSDDPCFLSAADLRRRISCKEISPLEVTRAVLARAERLQPELNC
                     FITLCADKALAQAKDAEHRIMTGEPLRILDGIPFTVKDIVNTKDVRTTFGAIPYTANV
                     PDHDAVGVARLRAAGAILIGKTTTPEFGTKCLTDSPLFGRTRNAWSRERSSGGSSGGA
                     AVAVASGIAPLAVATDGGGSTRIPAACNGVVGLKQSNGVIPHSQAQDAFGNQTYVTPM
                     TRSVADTVLMMQAMAGEDASDPWSIGIPPIDFIGGALPRGDLSGKKILFCLAPPGRKA
                     SADVAAAFSTSLKTLEALGAEFEEMPGDGFDVEPIWRAINHTVWRTRFERLAAEHRDE
                     LSETFLRQLALAQRTSGTDYQQAMFDRTILFRRVQSMLQRADLLVMPTISRTALPIGQ
                     DLFGTIDIDDEVFGDVRPNWFPWTMPFNMTGHPAVSIPCGFSADGLPIGLQLVGRFRG
                     DAELLRVCALFEAAQNWLDRWPDVVSTPSLTV"
     gene            24529..24780
                     /locus_tag="VGA15_14840"
     CDS             24529..24780
                     /locus_tag="VGA15_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589009.1"
                     /translation="MAIDEPQIDLITATPWVERWTDITQVMPPQLVVAIPKPEPMVTP
                     HGVVLVIITIILTLAIVEILFGGMINERMRKRRHRGFAR"
     gene            24806..25024
                     /locus_tag="VGA15_14845"
     CDS             24806..25024
                     /locus_tag="VGA15_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589010.1"
                     /translation="MTDEQLAAMHRLLAEYHRNLAKEAVLDIVQQYHTDLAQRLADEA
                     SQIPRRTAILARLHEREQQNIEYGDDGS"
BASE COUNT         5400 a         8145 c         7977 g         4932 t
ORIGIN      
        1 gaagacgcga aattcccctg gttataccgt tcaggtcccg gtcgttgata tcgtagaggt
       61 tggtgccggc ggaggctcga ttgcctggct cgacccgagc ggcaggctga gggttggacc
      121 ccaaagcgca ggatcgactc ccggtcctgc ctgttacggc aggggtggcg tcgatccgac
      181 agtgacggac gccaaactaa ccctcggaat cttggacccg ctgacctttg ccgagggccg
      241 catgcagctc gacgttgaga gtgcgcgctc agcaattgcc cgtgtttctt tgccgatgaa
      301 gctttccgta gaagaggctg cgctggcgat cgtcaaggtg gccgaggaat gcatgacgaa
      361 cgccttgaag ttgatcacta tacagcgcgg gcatgatccg cgcgaccttg cgctgatcgt
      421 atccggaggc gcgggacctt tgctggctgc cagcctcgga agggaactga acgtcaagcg
      481 gaccgtgatt cccacgcatc ccggtatttt ttcggcctgg ggaatgcttg ccgcccggcc
      541 gcgcgccgat cttcggcgga cggtgctaaa aacggtttgc gaggaggaaa tgaattcgat
      601 ctcacaactg tttgcggagc tcgaacagga agccaacgag tatttcgtcg gaaccagcgc
      661 gataaaacta aaattccaac atcgtgctga gattcggtat cgcggtcagg agcacagcgt
      721 ttcagtcggc ttttccggcg gcagcgccga ggaattgttg tctgacttcc atgcgaccca
      781 tcagaagacg ttttcgtttg cgttgaaggc gacggcggag atcacaatgc ttcatctaca
      841 gaccgaggtg ctcagcgaag tgatcggcct tccgaagatt caaggcaacg gagaagatac
      901 gctcgacagc gctctcaagg gccaacgctc cgttttcctg acaaaggaga agggttgggt
      961 cgcttgcaac gtttatgata gaaaccggct tccgatcgga tcgtcgattc cgggcccggc
     1021 gctcgtcgag gagccgacga cgacgacgtt cgtactatcc gatcagagat tcgctcgcga
     1081 cgagacgggt cagctcatca tgtggagccg atagttatct ctcgtatcag cggcgtcttc
     1141 gcctacacag atggggacgc gtcaagatcg ttcatgtcga accagactgc agggaccatc
     1201 caagcgttcg cgtaccgcac cagtttcgca ccagaaacga cctcgccaaa gcggaacgaa
     1261 cacgctcgat ctctgacgaa aatgcaaaaa aatcaacaaa ggtgagccgt atcctcccgc
     1321 tcataacggt ctggttgcag gttcgagtcc tgccttcgct cgcgaagcga gtgaaggctg
     1381 ccacgccgaa gccgctcggc gaaggcgggc cgcgctgccc gcgagctacg gccaggcaag
     1441 ccacgcgcga ccgttagggt gaagtgtgtc cggcgtagct tgagcgaagc gaaagcgaag
     1501 acggactggc gccgaacccc tcgcggcgaa gtcgggcccg ctgcccgcga gctacggctc
     1561 ggcaagccat ccccgcgatc atcatcggga aagaccgccg caaggccgac gcggctctcg
     1621 ctttcaacta gaatatcgaa aacaacccca tgcaaagtag catggtggtc gctggcatgg
     1681 atgctttgag cgatcccgcg aaaacatttt gacacgtcgg gcaaatcagc ggcactattc
     1741 catcatcgcg ccatttgtaa aacgcccatg gccctgcccg acaacgggct cttcggcgcg
     1801 attgcagaca aaaaatcctt ccgacaattg aagttgcacc ggctcgccac ggcgaatgat
     1861 cgcccgcgcg ttgccgaacc gcgcgcgctt ccgatgcccg tgcccgagga gatatcgacg
     1921 tgaacacagt tccagacctc aacaaggcag cggcaatggc cgcaaccgat caaacccggg
     1981 ctgccgcagc cccgcggata aagctcttgt cacacggatt ctccatcgac catccagatc
     2041 cggagcatgg cgaacagctg atgtcggacg cattgggggt cgcagatcgc gatgcgatgc
     2101 atggcatctt gagacaattg gtgaaagcca gcgtgaacgg gcaggagcct gacgcggtca
     2161 acctctcctt catgatttca atggtgaaaa gcatcaagcc cagggattcc gtcgaggcca
     2221 tgctggtggc gcaaatggtg tccgttcatg tgatggcgat gcgatgtgcc catcacctcg
     2281 caaacgcgga agacatcgct cagcaagaca gtgccgggcg cgcattgggc aggctggccc
     2341 gcacgtttcc ggcccagatc gaggcgctca gccgctatcg caacaatggc gagcctgcca
     2401 tcaccgtgca gaacgtgtcg gttcaggatg gcggcaaggc catcgtcggc aacgtcacgc
     2461 agcatgcgag cgtgatcgtc tcggacaaga gcccggcgcc cgcggtgacg gatgcccgga
     2521 tggtctcgat gcctgatcgt ggcgcaaagg agcacgatcc cgtggacgca aagcgggaag
     2581 caaaggcatg agcgaccacg cccgcaatac cgaaccgatg ctggcgagcc cgcgttgcgg
     2641 ggcaaaaatc cgctccggcg gatcgtgccg ctcgccggcg gtgcgcggca aaaggcgctg
     2701 ccgcatgcac ggcggtgcgc cggggtccgg cgcgccaagg ggaaaccaga acgcgcgccg
     2761 gcacggcctg ttcacaaagg tcgcaatcgc cgagcgcaag cagattcagg ccatgctggg
     2821 tgaagcgcgg aagttgctgc aagcgatgaa atgatctgct ttcgcagggc cgaacattgc
     2881 aggccgctga taacggtctg gttgcaggtt cgagtcctgc cgggcacctg cgtcaggcag
     2941 aaggcagacc tgccgacgcg agaagaacgt gtgtttttgt ttctcgatct cgtcggatcg
     3001 acgtcactcg ccgagtcgat gggtgagttg cgcatgcacg accttctcac tcggttcttc
     3061 ttcgacattg acgaggcgat cctcgcgcat ggcggcgagg tacatgcgta tgtcggggac
     3121 gaggtaatcg cgacgtggcg actcgccgcc aacatgtcag agcggcgtta cctcgactgc
     3181 ttttttgcca tccaggatag gattgcggag aaagcggatt tatatcgggg cgagttcggt
     3241 ctggtaccga cgtttcgcgc cggactgcat gctggctcgg tggtgatcag cgagtgcggg
     3301 gactcgcacc ggcagatcgc ctatttcggt gacacaatga acgtcgcagc ccggctgcaa
     3361 gagcgttgca aggaggccgg ccgtgctctg cttgtatctg ccgagctcct tcgccttgtg
     3421 cgtcctagcc gtgaccttgt tgtcgaagcg ctgggaccaa cgcagctgcg cgggcgtgcg
     3481 gcggctgttg aggtgttcgc cgtcgagcgc cgccgcttgg ccaatgaatg aaccggattc
     3541 gggacgcgca ccaggagtag ccgcttggct ccttgacggg ggaacgtgaa tcagtactca
     3601 gccattgaag gccgcctgag cttccggcag atcccagatc ttgccgatcg ccgcggtggc
     3661 ggccgaaatt atcgcggcct cgtcaccgtg aacatagtgg ccggcatcga tgagcacacg
     3721 tccatcaacg acgacctgat cgatattggc gcggttccca agcgcgacca tcccgcgtcg
     3781 cggatcgaac agaggctgca agtgtggatg ggtcaggtcg acgacggtaa ggtcggcggt
     3841 tgcgcccggc gcgatcatcc caaggtccgg ccgcttgatg agcgcggcgg cggttgcggt
     3901 gaccgactca atcaggtccg gcgcgctcgc tacttccgcg cgaccggacg caatcttcga
     3961 gatcatcgac gcggcgttga gttcaccgag aaggtccatg ttgtagccat cggttccgac
     4021 caccgtcctg acgccgtgct ccgcaaaccg gctgaacgcc gcggcgacgc ctgagcgcgc
     4081 aaacacgcgc gggcagttca gcaccgttgc gcctctcgcc gccatcagct tcaaatcgcc
     4141 atcggtgctc gcgatgcagt gcgccgccag aaggtctggc gcgaccaggc ccagccagtc
     4201 gagatattcg gccggcgtgc gattgccgta gcgcttccct atcgtcacga cctcggcatg
     4261 gctctgagca agatgggtcg tgatcggaac gtcgagttcg cgcgcgcggg cggcacacgc
     4321 ccgcagcagg tccggaccac aggtgtcagt cgcgtgcggg ctcatcgcga ggccaatccg
     4381 gccgtcgccg cgtccattcc agcgatgata cagcgcgttc caggtgtcga gatcgacttg
     4441 gccatcgtcg ccggcatagc gaaccactcc atccggaccg gccttggcat ccgacgtgga
     4501 gaacagataa ggagcgccat agaagcggat gcccatttcg tcggcggcgt cgaacatttc
     4561 gggtattgta ttgcgaaacg gctccatcac cgtggtggct ccgcccttca gcagttgcag
     4621 gatgccaagc cgcgcgatgg cgagccgctc tgagggcgac agcaggtcgg cgccgcgctt
     4681 ggtcagcggc agaagcaccg tatagacgat gctctggttg tttcgacgac cattgccatc
     4741 ctccgtgtgc gtccgagcga cggcttcgct gaagcagtgg ttgtgcaggt ttaggaggcc
     4801 gggcagcacg aaacggcctg gcttgtcgaa gatctcagta gcggacggcc ggtcgcgcgt
     4861 cacggcagcg atccgcctgc cctccagcaa cacccaatga tcgcgcagca cctcctgcgc
     4921 accatccttg cgcgacaaca cgtagctgcc aaagattgcg atcgttttca tgtcggcttc
     4981 acattccaga agacggccgt accgtcgagg ataccggtga tgctcgaacg gtacgcggtc
     5041 ggttgtttgt actgaccgat cgggaaataa ggaacttcct cgaacgacaa cgcctggatc
     5101 tcgcggcaga tacgttgttg gtcggcaaca ttggaggcta cgagccactg gcttcgcaac
     5161 gctcccatct tctcgctctt gtaccagcca ggagctttgc tgtcgccgcg cagattcgaa
     5221 ttgccagccg gattcagcca atcgatgcct tgccagttgc cgactccggc gctccaaccg
     5281 ccttgggcga tggggtcctt tttcaactgt cgctgcaaga ccacgccgaa gtccagcccc
     5341 gcatattcga cgttcatgcc ggcctgctgc agcatgtcgg ccgccacgtc gccgagtggt
     5401 ttctgtgcga gcgagttggt gggaacgagc agcacgactt tctcgccgtt gtaaccagcg
     5461 gccttgaggt cagctctgac cttcgccatg tcgcgcggtc cgcggaacac attcaggccg
     5521 acatcgctcg ccatgggcgt accgggcgca aagaagccga tcggcgagga ttgataggcg
     5581 ggatcgtcgc ccgcaaccgc cgtcatgaaa gcggactgat caatcgcacc caacagcgcc
     5641 cggcggatgg ctggattgtc gaaaggcggt tgcagatggt tcacgcgcag catgcaggtg
     5701 tagccgcgcg ggtcgagaat ccgcgtggtg atgccgccgg cacgcttcag caccggcagc
     5761 aggtcgtgcg gcgttgtttc ctgccagtcc tgctcgcgcg tttgcagcgc agcaacccct
     5821 gtcgccgcat ccggcatcgt cgtccagacc acgcgatcgt aatgaacgat cttgggaccg
     5881 gcggtccaat ccggcttgcc gtcttggcgg ggctgatacc gatcgaagcg ggcgtacacg
     5941 ttgcgcgcgc ccgggacgcg ctcgtccgca acgaagcgga aagggccgct gcctacgact
     6001 tcggtcagcg gcttgaacgc gtcctggctc gccagccgct cgggcatcat gaacggcgcc
     6061 tggatcgccg atttgccgag cgccgcgggc aacaaaggga aaggcctgtt cagtcgaaag
     6121 cggatggtac ggtcgtccgg cgctgacagt tcgtcggtcg ccgccatcag ctcggcgccg
     6181 aacccgtcgc gggtggccca tcggcgaatg ctcgcgacgc aatcgcgcgc caggacgcgc
     6241 tcgccgtcat gccatagcaa accgtcgcgc aacgtgagat cccactgccg atcgtcgctg
     6301 gagacgacat gcccggacac catctgaggc gagacctcga gccccgaatt catgccgtag
     6361 agagtgtcgt agaccataaa tccatggttt cgggagacct gcgccgtgga gtagatcgga
     6421 tcgacgaagg tcagatcgat cacaggaatg aagcgaagcg tgctctgcga ctgagcgcga
     6481 agaattctcg gcagcgaaaa cgccggcgct gcgactgcgg cccgtaatag tgaacgacgg
     6541 gaaacgagca tcgaggactc ctaagatgag aaacgggtaa gacggccaat ggggctgccc
     6601 gggatacgtg atcggcgcga atctcaggga gtggctgcct cgtgactttg gaaattcgcc
     6661 gcgatcgcct cgccggttgc gatccacaga tcggggcagg atttggcgta ggttagaaat
     6721 tctcgcagca gacgaagccg catcggacga ccgctgcatt gcgggtgcag cacggtcgtg
     6781 accatcgcgc cccaatcccg gacctcgtcg agttcatcct tccagatcga gagcacgtgc
     6841 tccttgggaa agatcgaccg tgggctgaat cgggcggaca gtccgtgcat ccagtcgtcg
     6901 tagctcgcgg tcaccggaag ctcaatcgtg ccaggcctgc catccgcaag gcgatggcgg
     6961 taaggccgca cgtcgtcccg aaacgacgag gtgtaaacga tgccgtggcg caccaacgcc
     7021 acgcgcagct cttcggtgaa ttcgccgtaa ggcgctcgat agccggttgg cttgacacta
     7081 agccgacgct tcagtgcctc gaagccccgc tccagctcct cctcgatcca ggggtcgccc
     7141 ggatccggca gcagatgatg atacccatga tggccgatct catggccggc cttgagaatc
     7201 gcctcagcca tcgcgggatg cgcatccgcc gaccagccgg tgatgaagaa cgtcgccttc
     7261 agatcaagct gatcgagcaa ctccagaagt ttcggcgtgc cgacacgcgc ctcgtagccg
     7321 ccataactca tcgtgatgag gcgctgggcg tgagctgcgt ccttgctggt ccatgcgctc
     7381 tccgcgtcca cgtcaaatga caggaacatc gcggaagtat acggcgccgg ccaaggatac
     7441 tccggggccg gcggcgcggt gttcgctgga atgagcggaa tgctcgcgag cgctttactg
     7501 tcctgcattg atcgtcgcct ttttcacgcc gtaatcggcc aatttccact tcgcaagcaa
     7561 aactcggtag gagccgtccg cgatcaacgc atccaaggcc tccacaaccg cttgctgaag
     7621 cgccttctcc ttgacgctca aagcaagccc ggtgaactgg agggcgaaaa catcgccgat
     7681 cgggatatac actccgggtt cgaggtccat catgtaggga agtgtctcgc caccctggac
     7741 ggcagcgtcg agccgccctt gcttgagctg ggtccgggca tcggccgatc cttcggtgcc
     7801 gacgaatata atcggatcgc cgccgcaatg cgcatcgctc caggcggcga ccagcttcgg
     7861 aaaggacgtg cgccggctgg cgcccacctt tttcccgcaa agcgccatcg ggtccttgaa
     7921 ctccgctgcg cgagattgct gggcgaagaa tcgaggccca ttgcgaaggt agtcgacaaa
     7981 ggtcgccgtg tcctgccggc tcgccaggtc cgtcatgccg gacaggatcg catccaccct
     8041 ccccgtcgcg atcgcgggca tcatttggtc gaagctggtt tcctgccaga cgatcttgac
     8101 gccgagcttt cggccgagcg cctcgccgag ctcgacgtcg aaaccagtca gcgtgttggt
     8161 agcgggatcg cggaattcca ggggtggata gttcggcacg atggcggccc tgatgctgcc
     8221 ctgcttttcg atttccggag gcagttcgac cgcgatggcc gaaccagtgg cggcgcagag
     8281 gaacgcagca gcaagaaaac gtttcatcat gaaaatcgcc tgatcagatc gccgcggaga
     8341 gaaaggactg ggtacgcgct tcacggggtg caccgagaac gtccgctgcg gggccggatt
     8401 ccacgatcgc gccatggtcc atataaacaa ccctatcagc tacctcgcgc gcgaagccga
     8461 gttcgtgcgt taccaccatc atcgtcattc cgctgcgggc aagctccttc atgaccgtga
     8521 gcacctctcc gaccagttcg ggatcgagcg cactcgtcgg ttcgtcgaac agcatcagca
     8581 tcggcttcat ggcgagcgag cgcgcgatcg ccacccgctg ctgctgcccg cccgaaagct
     8641 gcgcgggata ggcatcggct ttcgccgcga ggccaacgcg ccgcagaagc tccatcgcct
     8701 ccccccgcgc gtcctcccgc ttgcggccct gcacctgtac cggaccttcg gtgatatttt
     8761 ccagcgccgt cttatgcggg aagagattga atcgttgaaa taccatgccg gtcttcagcc
     8821 gctgccgggc gatctgctgc ggggtcaggc gatgcaagcg cccgccctct tcgcgcagcc
     8881 ccagcaattc gccatccagc cagatgccgc cgctgtcgat ggacgtaagc tgattgatac
     8941 aacgcagaag cgtcgtcttg cccgatcccg atgccccgat caggcaaagc acctcgcccg
     9001 cccggacgtc cagcgagacc cgcttgagag cttgaagctc cccgaaattc ttgctgaccg
     9061 aacggatcgc gacgaggggg cgggtcatcg ggctatctgc aatgaaccgc gggcgaagcg
     9121 tcgctcaagc agcatctggg aaggcgtcaa gatcgacacg acgagtagat accagaagcc
     9181 ggcgacgatc aggagttcga tcacgcgcga attcgcgtaa tagatattct cggcgttgtg
     9241 cagcacctcc ggatactgga tgacgcttgc gagcgacgtg gccttcacca tgccgatgaa
     9301 ttcattgccg agcggaggga tgactacccg catcgcctga ggcaggatga tccggcgcag
     9361 cgcgtgcagc cgtcccatac cgatcgcctg cgcggcctca tattgtccgg cgtcgaccga
     9421 cagcatgccc gcccgcatca cttccgacgt ataagcgccc tgatttatac cgaggccgag
     9481 aagtgcggac agaaacggcg tcatgacatc gaccgcccgg gccgaccaca ggccgggaat
     9541 gccgatcgtc ggaaacacca gcgcgatgtt gaaccacaac agcagttgca ggatcagcgg
     9601 cgtgccgcgg aacagccacg tatagccggc ggcgaccgac ttgagcaccg gattgggcga
     9661 gagcctcatg atcgcgacga cgacgcccaa aacgatgccg agagccatcg cgagcaccgc
     9721 catcaccatg gtgttgacga tgccttcaag gatcaccttc gcggtcagaa agcggccgac
     9781 ataggcccac tcgatctgac cgttggcgaa ggcacgcccg atcgcgacca gtacgacgag
     9841 aattgctgcc gcggcgagcc atcgccgcca atgagcctga cgcgcgagcg tcatccccga
     9901 aatatcgggg aagcctcccg caagcgtgtc cgccgctctc attgcgtggc cgcattcgtc
     9961 attggttggg gcaccgcgtt cgcacttaac ccgtatttgt cgagaatggc cttgtaggag
    10021 ccgtcggcga tcattgcggc aaggtgttgc gtcaccacct cgcggagcgc cgcgtcgtct
    10081 ttccgaaaca tgatgccttg atagccggtg gagaacggct ctccgacgac gcgatatttc
    10141 ccggcctcct gctgctgggc gtagggcagc gtctcgctgc cctgcaccgc ggcgtcgatg
    10201 cggccttgct tgagctggtt gcggacgtcg atgctgttct cgcccggaac atattgcacg
    10261 gcgggcttgc cgccggcctc gcagttctgc ctgctccact tctcgatctc gatcgggaag
    10321 ctggtgctgc gcgtcgttcc cacccgcttg ccgcagagct caatggcgga cttcgccgcg
    10381 ttctccgtca ggacgaagat ctgcggacct gtcgtcagat agtcgacaaa gtcggccgtc
    10441 tcgcgccgcg agctgcgatc tgaaataccg ctgatgatga aatcggcgcg cctggtctgc
    10501 agcgagggga tgagttcggc gaatggtgtt tcgctccaga cgatcttcac acctagccgt
    10561 ttcgcgatct cgtttgcgag atcgatgtcg agtccgacga gttcgttcgt cgctggatcg
    10621 cgatactcca tcggggcata agtcgaattg accgtcagcc gcaatacgcc cgcctgcttg
    10681 atcgactcgg gcagttcggc agcctgtgca attgtaaagg cgaggagcgc ggcgaaacca
    10741 acggagaacg aaaggcgtgt catatcagct ccttcggccc gcgcgagtgg ccgcatcata
    10801 ttgattcgca tcgagagcgg cgggttcaat gacgccagca cgctccgtga tcaccgtgta
    10861 ttgctccggc cgccgatggg cggcgaaatt gaacatcttg tccttgccct ggcgacagaa
    10921 atcgagatca aggtcggcga cgacaacttc atccgcgagg gtctgcgttt cggcgacgat
    10981 ccggccgttc ggatccacaa tgcaggagcc accgatcagt ccggagccat cctcgttccc
    11041 cgccttggcg accgcgatcg cccaggtggc gttcatgtag gcgttggcct gagtcacgag
    11101 cttggaatgg aaggtccgca gcgccgcatc ttctgtgacg ccgccgtttg gatcgtaagc
    11161 cgcggaattg tagccgacgc agaccagctc aacaccctgc aacccaagca cgcgccatga
    11221 ctcgggccag cggcgatcat tgcagatcat catgcccatg atcgcatggc gccaatccgg
    11281 ccccgtccga aaagcaggaa atcccaagtc gccatattcg aaataccgtt tttcgagctg
    11341 ctggtagcgg gcaccggcac gtggttctac cgagcccggc aagtgcacct tgcgatagcg
    11401 cccgagaatt tcgccgtcgc cgtcgaccag aatcgaacaa ttaaagcgcc gacggtccga
    11461 tgtcagttcg gcgtaaccga catagaatcc gatgcggagc gcccgggcgc gatcgaacag
    11521 gcgctgcacg gccggattcg gcatacttcg ctcgtagtga tgatcgagcg ccttgccctc
    11581 aacgagccac cgcgggaaga aggtcgtaaa cgccagttca ggaaaaacga ccagacttgc
    11641 gccgcgcgca gccgccgcat ccaggagctc cagcatccgg ccaagcgtgt gctctcgcga
    11701 gtcggcattt tgcgtcggcc ccatctgagc agcggcggcg cggagaactc ggaccaacat
    11761 tgcctccaat ctgtgcggat tgcttactca agaggcggat gagcccgctc cgacagcgct
    11821 ccgatgcgcc ggtcattacg cggaagggcg gcaccaattg caatcattcg tgctatgata
    11881 ttttcagtga aacaataacc ggtagttata tgaatctgcg ccagttggag atcctgcgtg
    11941 cagtcatccg ccatcggacc acggtcgcgg cagcggacga actggcgctg tcgcagccgg
    12001 cgatcagcaa cgcgctgaag gcgatggagg cgcaggcggg ctttgcgttg ttcgagcgcg
    12061 tcaacaaccg gctgttccct accgccgagg cgatggcgct ttacaaggaa agtgaagcga
    12121 tctttgcgct ccatgcgaag ctcgaaaatc gcgtgcgcga tctgagggag tgtcgctcgg
    12181 gacatctgtc gatcgtcgcg accccgccgc tggcctacag cattattccg cccgcgctgt
    12241 caggctttct gcgccgccgt cagcagacgc gtgtattctt cgatgtacgg cgctacgaag
    12301 gaataatcga cggcgtgctg agcagggtag ccgaactcgg tttcgcgctc ggtctgtcgc
    12361 atcatccggg cattgcgcac gaggtagttc acaccggcga aatggtctgc gtcttgccgc
    12421 cgaagcatcc gctggccgag cggccgatca tttccgcctc cgacctcgtc ggcttgccct
    12481 tcatcgggct ggagcgcgga acgcgattgg gcgaagccgt acgcgagagc ttcgcgcaag
    12541 ccggcgcgcc gttccagcca accgtcgagg tgcgatattg caatacggcc tgcgtgctcg
    12601 cggccgccgg cgtaggcgct gcggtcgtcg atccgttttc gccgcgccag ggcggcagcc
    12661 acgacctaat cgtgcggcct ttcgcgccca agacagtggc tgtggcctat atgcggtggt
    12721 cggaagcgga acctctatcg cgtcttgcca agacattcct gaatgaagtc cggcaagcga
    12781 gcggcctgct gggccgcgat ggctgctagt ctagcctgat gcactcaggc tgcctgtcgc
    12841 cccggccgac gtcgcggtct ccgcgcacaa cgccgacgag atcggcaaga tcgtgaacag
    12901 cctggcggcg aaaggcgtca agtcctgggg taagccgatc gatgtcgctg atccggccgc
    12961 actcgacgat ttcggtcacc gacggaaagg ccgcccctgt gcaataacgc tcaccccaag
    13021 gcctatctcg gttccaccca tttcttcttg ccgagcatgc gttgccagat gagcaccgcg
    13081 acttgccgat cgccatcgaa ggcaagcgaa gcaccggccg agaccacttc ggcagcttcg
    13141 tcgggagatt ggatccgaag taccgtcaca cccatgagtt cgaatgctcc gggcgtggcg
    13201 ctgcctatcg gaatttgcca cggattgaac tctgcccact cgccgcccat cgtgacgggc
    13261 gtaagcagcg gcattcgatc gcatcggtac tcgcgagggg ctatccagca gcaagtggat
    13321 cggcggcgcc gtgcacgggg tgagcgccca cggcaagatt aggccggcga tgatcgcggt
    13381 gccgagcgga catcgcagtt ccgaggtagc ccgactgcga tgaaggtctg ctttgcgcca
    13441 tttgcagtgt cattcgcaca ccagatgtta tggagcgtcg atgtccgctc ttctcaacag
    13501 cggacatccc ccgcatcggc caaaatcttc aaattacttt cgctgggacg taggcgatct
    13561 cgattggatc aggtatggcc ccgtctaatc aggtttggca atctgacggt agagatcagc
    13621 atagtggcgt gcccgattgt gccacgctac atcaacggtc atgccattct tctgaatcct
    13681 ttgccaagcg ggcttgttgt gaaacagccc tttggcgcgg cgaacggcgc cagtgagatt
    13741 ttcgctcgtg actggcccga actgaattcc tgtcgctaca cctgcaggga tggtcatctc
    13801 gttggcgtcg ataacggtgt ccgctagtcc gccgacgcgc gatacgatag gaatagcacc
    13861 atagcgcaag gcgcatagct gcgtcagtcc gcatggctcg aaacgcgacg gtaccacgag
    13921 cgcatctgcg ccggcctgaa tgagatgagc cagattctca tcatagccga tcaaaacgcc
    13981 gatctgatcc ggataggatt tggtggcagc aatgtagcgc gcctcaaggt cccgatcgcc
    14041 atttccgagc aaaaccaact gcacaccctt gcacaaagcg agcggcagac tctccaggag
    14101 cagatcgagc cccttctgcc aagacaagcg actgataact ccgagcaaaa atgcatcggg
    14161 tgcgagggga aggtcgaaac gcctttgcaa cgcggtttta ttcgccgcac ggtcggcaag
    14221 tgtatttacg ccgaacggtg atgcgatgcg cggatcggtc ccgggattcc agacggctat
    14281 gtcgataccg ttgaggattc cgctcaacac atccgatcgt gcgcgcagta gcccatcgag
    14341 gcccatgcct gcatcaggct cctgaatttc gaccgcatag gtcggcgaaa ccgtggtgat
    14401 acgatcggca aattgcagcc cggccttcaa aaagccgatc ccaccataat attcaactcc
    14461 gtcaatcgtg aaggattcgg ggggtagtcc gatatccctc aacatctccc gcgggaattt
    14521 tccctgatag gcgagattgt gaactgtcat gatcgtgcct ggccgcggcc gctcgctgta
    14581 gtgcaagaag gcgggggtca gcccggcctg ccaatcgtgc gcgtgcacga catgaggcac
    14641 aaatgccggg attgcgcctt cgccgatttc ggcagcggtt cgccctagcg cagcgaaacg
    14701 aatggcgttg tcgggccagt cagcgccgtc gggcgcaaca taagggtttc cgggacgcgc
    14761 aaataaatgc ggcgcgtcaa gtatgaacaa gtcaagctcg gccatggaac agctcagcac
    14821 acgcgcaggc cccccataaa aatttgggaa gcgaaggact tcatctgccg attccagctc
    14881 gttcagcaca tccggatatc ccggcaccac agtacgcgct tcgatccctt ccgacttcaa
    14941 agccttcggc agcgcgccag tcacatcggc gaggccgcca gtcttgataa gaggatagat
    15001 ctctgaagca accgaaagca cgcgaagggg cttcatgcag acagcctgtc gatcatcggc
    15061 tgcgtaatca ggcagattcc ttgctcggtg cggcgaaatc gcttggcgtc gcattcaggg
    15121 tcttcgccca ccaccagtcc tttcggaatc cgaacaccgc ggtcgatcac cacgttcttc
    15181 agtcgtgaac cgcgagccgc ttcgacatac ggcaggatca ctgcattttc gattttggca
    15241 aaggagtgaa catgcacacc ggtgaacaac agggaacgcc gtatcgccgc gccagatatg
    15301 atgcatcctc cggagacaag cgaagtcacc gcctcgccgc gccgaccacc ccgatcgtga
    15361 acgaacttcg caggcggcgt gatttcggca tgggtccaga tcggccagga gcgatcgtag
    15421 aggtcaagtt caggcaagat gtcggtaaga tcaatattgg acgcccaata agcatctagc
    15481 gttcccacgt cccgccaata gctgcgcgcg tcattatgtg accgaacgca agagcggctg
    15541 aaatgatgag ctacggcgcg cccatgtttg acaatataag ggatgatatc cttaccgaag
    15601 tcatgactcg aattcggatc ttctgcatcg cgccgaagct cttcgaaaag gaattctgaa
    15661 ttgaaaacat agattcccat gctggcgagc gatttatcgg gcttgcccgg catggctggc
    15721 ggatcggttg gtttctccag aaacgatttg atgacgtcgg tatcgtcgat atgcatgatc
    15781 ccaaagccac tggcctcgct gcgtggcacc tcctggcagc caatcgtcac gtccgcgccc
    15841 tgctcgacgt gctgttgcag catgagctca taatccatct tgtagatgtg atctccggcg
    15901 agcagcacga taaatttggc gttgtggggc tcgatgatgt cgatgttctg gtaaactgcg
    15961 tcggctgtcc cgagatacca gttcttctcg gaaatgcgct gactggccgg cagaatatcg
    16021 aagctttcgt ttcgttcggg acgaaaaaaa ttccaaccca tttgcaagtg tcgaatgagg
    16081 ctatgtgcct tgtattgggt ggcaaccgcg atgcggcgga tgcccgaatt catggcgttg
    16141 gagagggcaa aatcgataat gcgcgacttg ccaccaaaat agacggccgg cttggcgcgt
    16201 cgatccgtca attccaaaag ccgtgagccc cgtccgccgg cgagaacgaa ggcaatggca
    16261 tgacgcgcaa ggggctcgtt tccggctggc cgcatgggca tcctcctccc aggagaaaat
    16321 cagtttttta attctctagc gcagttcgca tacgatttct tcaatctaga tcaatttcga
    16381 agcagcacgc tagtgcagga ccggctcaat ccctcgaaaa gccccgcgaa ccctgatgaa
    16441 ccagcccgct ccaaagccac gaagatcata ttctcaaacg ggtgtccgct ttgcgcctgg
    16501 acatgcaggc atatcccgtg aattgagcgg caaaggggcc gcttattcgg cccgattgcc
    16561 agtcgtcgtc gacggcatcg gccctggaaa aatgggctca aggccatcag caagcctcct
    16621 ctacgtctcg acatgaaatt cgacctggca ggttcattcc caagcccaat ccgagaaatc
    16681 gttggcaaac tggggtacct ggccccacac gcccttcaac ttcttgttcg cgatggtgat
    16741 caattgcggt tggtacagga agccggcgac ggcgtcggtc gcgagcatgt gctgggcatc
    16801 gcccagcagc ttggcgcgat cggcatcgtt ggcggcttcc gtgatcgact tgtagagggc
    16861 gttgaacgcc tccgatttgt agccgatata gtagttatcc tccgtgtatt tcacgagatc
    16921 gaacggttcg acatgggaga cgatggtgag gtcgtagttg tgcggcccgt tgaacaccga
    16981 cgacagccac tgcgcccatt ccacattctc gatcttcgcg gtgatgccga tcttggcgag
    17041 ctgtgccgcc agcacctcgc cgccctgccg cgcataagcc ggtggcggaa gtttcaggct
    17101 cacttcgagt ggcgtcgtca cgccggcttc ggcgagcagc ttcttcgcct tctcgacatc
    17161 gaaaggattg atatccgtcg tgtcgatgta gccaagcgag cccggcgtgt agaaactgcc
    17221 gatcggcacg ccgaagccgt cagccgcacc ctcaatcatg gctttccggt cgattgcggc
    17281 gaggatcgcg cgccgcaccc gcacgtcgtc aagcggcttc ttcttgttgt tgatggcaac
    17341 gatggttttg gcccgcgagc cgccgatctg caccgcaaat ctcggatctg ctttgaattg
    17401 agccaggctg cgcgcggcgg cgacacgggg aaaggcatcg acgtcgcccg aaagcagcga
    17461 tgccacctgc gcagacggat cgctgatgaa gcggatcaag atcttggtga gatggatcgc
    17521 cgccgcgttg cggtatccgg gccatttggt caatgtgatt gacgaccctt tcgcccaggc
    17581 agctagcacg tatggcccgg tgccgaccgg cgccgtggca ttggtcggcg cgcttttcgg
    17641 ttcgacgatc gaggctgagg cctgcccgag caagaacggg aggttgggtt cggggttctt
    17701 catgttgatc acgaccgtgt caccgcccgg cgtagtgatc tgggcgatgc tctggaacag
    17761 gctcttgtcc ttgttggtgc tggtcgaggc cgccgcgcgc tcgtaggaga acttcaccgc
    17821 ctccgcgttg aacggttcgc cattctggaa cgtcacgccc ttgcgcagct tgaacgtgta
    17881 ggtcttcaag tccggcgcag tctcaaagct ttcggcaagc aacggcacaa ccgagccgtc
    17941 ttcccgaacc ttggtgagcg tctcatagac gttgtagagc gtgacctccg caatggccgc
    18001 ggcggccccg ctggtgggat cgaggcccgg cggctccagc gtcatcgcca tcaccacact
    18061 gtcttttttg ccctgggccg agagcgattg cggcagcagg caaagcgctg tcatcgcggc
    18121 agcagcaagc agaacgagct tctttaacat acgaacgctc ctaaacggga cgagccgatt
    18181 ggcgcatcaa actgatatcc atcgcattag actatcacac ctcaaccgcc aagggaggca
    18241 gcgccatgat ggtctcggcg tggtgacagg cggaggtatg gtcgggaccc acgctgcgcg
    18301 ccatcggcgc gaccgcgcgg caatgcgcgt cggcgaacga gcatcgcccg gcataggcgc
    18361 agcccgtggg tcccaccgat tgcgacgcga ttgcctgcgc gccgcggcgt cgccgctcgc
    18421 gccccggacg gatgcgcggc acggcttcca gcaacgcgcg tgtgtaggga tgagcacaac
    18481 ggctgaaaag atcctgcggt ctgccctgct cgacgatgcg cccgaggtaa attaccgcga
    18541 tctcgtcgca cagatattcc accaccgcga ggtcgtggct gatcaagacg tagctcaatc
    18601 cgaactcgtc ctgcaggtcc tccagcagat tgagcacctg cgcctgcacc gagacgtcca
    18661 acgcgctgac cggttcgtcg gcgacgatca gttttggctg cgtgatcagg gcccgcgcga
    18721 cggcgatgcg ttggcgctgg ccgccggaga attcgtgcgg gaacttgtcc atatcggcgt
    18781 cgcgcaggcc cacctgcttc agtactgcgg cgacgcgggc gcgctgctcg gcgcgatcga
    18841 tgtggccgag cgcggtgagc ggttcggcga cgattcgcgc gactgtctgg cgcggatcga
    18901 gcgagccgta gggatcctga aacaccatct ggaaatcgcg ccttgcgcgg cgaagctcac
    18961 cggccggcag ccgatgcagg tcgcgcccga gcaacttaac cgagccggat gtcggtgtct
    19021 ccagcgccat caccgcgcgc gcgagggtgg atttgcccga accggattca ccgaccacgc
    19081 cgaggctctt gcccgcggcc agttgcacac tgacgccgtt gagcgccagc acctgtgccg
    19141 ccgcctggaa caggttttca cgcggcaacg tgtatcgctg cacgaggtca tcgacctcca
    19201 gaagcatcgg ctcgggagca acgccagcag ttgtcgtcgt gctcatgcgg tcacggctcc
    19261 aatccggttc gccatcgaga cgtcagtacg caggcagcgc acggtgtggc cggcattgac
    19321 cgtgacgggt ggcggaaaga tcgcccggca gcgctcttcc gcgatatcgc aacggtcggc
    19381 gaaggcgcag ccggaaggca ggtctgcgag ctcaggtaca gtgccgggaa tggtttgcaa
    19441 ccgggtacct ttcggcgcgc cgagccgcgg tcgcgcgcgc aacaggccct ggctgtaagg
    19501 atggcccatg ccagacaaca gcgcttcgat cgtcccgttc tccaccaccg tgccgccata
    19561 catcaccatc atgcgctgta cattttcggc gatgacgccg agatcatggg aaatcaggat
    19621 catggacatg ccgcgctcgg ccatcagatc ggcgatcagg tcgaggattt ggccctggat
    19681 ggtgacgtcg agagcggtgg tcggctcgtc cgcgatcaga aggtccggct cgcaggcaag
    19741 cgccatggcg atggtgatac gctggcgctg gccgccggaa aactggtgcg ggtaggcagc
    19801 ggcccgcttg gccgcatcgg gaaggccgac gcggtcgagt agtgcaatcg cttctctgta
    19861 cgcctgtgcg cgcgaaaacc ccctgtgccg gcgcagcggg tcggcgacct ggcggccgac
    19921 ggtgtgcatg ggattgagcg ccgtcatcgg ctcctgaaag atcatgctca cgcgattacc
    19981 gcgcagtttg caatagtcgg cttcgtcaag gcctaccagt tcctgaccgt ccagccggac
    20041 gctgccgcta accacggcgc tctccggcaa cagccccatc agcgccatcg cggtcaccga
    20101 cttgccgcaa ccggactctc cgatcaggcc aagggtctcg ccccgcgcca gcgaaaagct
    20161 gacgccacgc acagcgtcgg ccgggccacg gctggtattg agccggacgc gcaaattatc
    20221 gacttcgatc agcggctttc tgtcggtcat caccgctccc gcgacagccg cggatcgaac
    20281 aggtcccgca gtccgtcgcc caagagatta agtcccagca cagcggtcgc aatcgccaga
    20341 cccgggtaga ccgcaagcga cggcgactga aacaaaaggc tctgcgcgtc attgagcatc
    20401 cggccccagg atggttgcgg cggctgggtg ccgagcccga gataggagag cgccgcctcc
    20461 gccaggattg ccaacgcgaa ctggatcgtg gcctgtacga tcaggatcga gagaatattg
    20521 ggcagcacat gctcgagggt gatgcggaat ttgcccttgc cggaggcgcg cgccgccaac
    20581 acgaactcgc gcgcccagat cgcgttggcg gagccacggg tgatgcgcac aaaaatcggg
    20641 atctggaaga taccgatcgc cgtgatcgac gtgaccatgc ccggccctgc cacggcagcc
    20701 agcatgatcg ccgaaagcag cgccggaaac gcaaaggtga agtcgctgag ccgcatgatc
    20761 gcctcctcga cccatcccct gcgggcggcc gcaacaagcc cgagcgaggc gccgaacaag
    20821 agaccaatgc ccaccgcaat gatgccgacc acaatcgagc ttctggcgcc caccagcagc
    20881 agcgagacga tatcgcggcc cagcgtgtcg gtgccaagcc agtgcagcgc cgaaggcgga
    20941 ttgagcttgt gcgccatgtc gatctcgtag gccggccacg gagtccagac cagcgacagg
    21001 agcgccgccg agatcatcag catcgtgagc acgccgcccg ccacgaagct gcggtgacga
    21061 cgcgcacgtt gccagaaact gttgccggat cggcgcgact gcgacaaagc cgcagcgtcc
    21121 gggggagcgg cgttcgacgg cgtcgctgct gcgggttgaa tgccgctcat aattcgctgg
    21181 ctttgatgcg cgggtcgatc gccgcataga gcacgtcgac cacgaaattc acgatcacga
    21241 ccatcgtcgc cagcagcatc acgcaattgc ggatcacgat gagatcgcga tttgagatcg
    21301 actggaaaat gaggcgcccg agtccgggta ggtagaagac attctcgatc acgatcgccc
    21361 ccgcaagcag gttggcgaat tgcagcccca tcagcgtcat gaccgggatc atcgcgtttc
    21421 gcagcacgtg cccccacagc acctggcgcc ggctgaggcc cttggcgcgc gccgtgcgca
    21481 cgaaatcctc ccgcaacaca tcgagcactg cggagcgcgt gacccgcgcc aggatcgcgg
    21541 cctgcaccac tgcgagcgaa atcgctggaa gcagcagcga gcggataccg ggaagaatgc
    21601 cctcccccca tcctacgaac ccgccggccg agagccattg taacttcacc gagaacagca
    21661 ggatcagcag gatggcgaac cagaaattcg gaatagctat gccgaattgc gtcgccgcca
    21721 tcactccgat atcaccgatc ttgttgtgat tggcggcggt gtagacgccg gcgcccagcg
    21781 ccagtaccac tgtaagcagc atcgccatga ccgccagcgg aatggtcagc gtcagccgct
    21841 cggcgatcag atccgccacc ggcgtgccat aggaatagct gttgccgagt tcgccgacca
    21901 gaagtccaac aatccagtgc agatagcgaa cgaccagcgg ctgatcgagc ccgagtttga
    21961 cggtcatcgc gcggatcgcc tccggcgagg agtccgcgcc catcagcatc tcggcggcat
    22021 tacccggcaa cgcatcgagg acgagaaaga tgatcagcga ggcgcccgca agcgtcgcaa
    22081 ccagtgtcag aaggcgccgg acaatgaaga cgctcaccga aggttccctt cgcgcagaaa
    22141 ttgttagccg gaagacatcg catcgtggat catccaatct ttgcgaagat cattcaaata
    22201 gaacttgcgc gaactccggt ataccgcacc gaggcgcaac cttcgcatcc gtcaaacggt
    22261 cagtgacggc gtcgaaacca catccggcca gcgatcgagc caattctgcg ccgcttcgaa
    22321 cagcgcgcag acgcgcagca attccgcatc gccgcgaaac cgccccacca gttgcaggcc
    22381 gatcggaagc ccgtcggcgc tgaagccgca gggaatgctc accgccggat gtcctgtcat
    22441 attgaacggc atcgtccagg gaaaccagtt cgggcgaaca tcaccaaaca cctcatcgtc
    22501 gatgtcgatg gtgccgaaca aatcctggcc gatcggaagc gcggttcgcg agatcgtcgg
    22561 catgacaagc agatcggcgc gctgcagcat cgattgcacg cgtcggaaca ggatggtgcg
    22621 atcgaacatc gcttgctggt agtcggtacc actggtcctt tgcgccagcg ccagctgcct
    22681 gaggaaggtt tcgctcaact cgtcgcgatg ctcggctgcg agcctttcaa atcgggtccg
    22741 ccagacggtg tgattgatcg cgcgccagat cggttcgaca tcaaagccgt cgccgggcat
    22801 ctcctcgaat tcagcaccga gggcttcgag cgtcttcagg cttgtgctga atgccgcagc
    22861 aacatcggcg gaagccttgc ggcctggagg cgcgaggcag aacagaattt tctttccact
    22921 gaggtcgcca cgtggcaggg cacctccgat gaaatcaatt gggggaattc cgatcgacca
    22981 gggatcggat gcatcctctc cggccatggc ctgcatcatc agcaccgtgt cggcgaccga
    23041 acgcgtcatc ggcgtgacgt aggtctgatt gccgaaggcg tcctgcgcct gactgtgggg
    23101 aatcacgcca ttgctctgct tcagcccgac aacgccgttg catgccgccg gaatgcgggt
    23161 ggaaccgccg ccatcggttg caaccgcgag cggcgcaatg ccgctggcga ccgccaccgc
    23221 cgcgccgccg ctcgagccgc cactcgaccg ctcacggctc caagcgttac gcgtacgtcc
    23281 gaacaagggt gaatcggtca ggcatttggt gccgaattcc ggcgtagtgg tcttgccgat
    23341 gagaatagcg cccgctgcgc gcagccgcgc cacgccaact gcgtcatggt cggggacgtt
    23401 agcggtgtaa ggaatggcac cgaaagtagt acgcacgtct ttggtattga cgatgtcctt
    23461 tacagtgaaa ggaataccgt ccagaatccg aagcggctcc cccgtcatta tcctgtgttc
    23521 ggcatccttt gcttgcgcca aggctttgtc agcacagagc gtaatgaagc agttcaactc
    23581 aggctgtagc cgctcggcgc gcgcgagtac ggcccgtgtg acttcgagcg gcgaaatctc
    23641 tttgcaacta atacgccgtc gaaggtcggc ggcggagaga aagcagggat cgtcgctcat
    23701 ctataaaccc aattcgtccc aagaagccgg cttcggttct ggctcgattg tcgaacactt
    23761 tccgccaagc ggggttgcaa cacgactacc tcatgaccca gtattaacac gcctaactat
    23821 ccggctcgcc caataacaga gccgaacccc gcaatccgcg aaccacccga atgaacccaa
    23881 agcgccaatc ggattgatag taagaacgac actcttgtca attcgccgtc caatacccat
    23941 tccgcgccaa cccatgcggt cctttggatc tccgccggtt tcactatttc gttgccgtgg
    24001 ccgaagagcg cagcgttggc cgagccgcgg aacggctgcg cacgctactg gatcgtaccg
    24061 tgacggcggg cgcatggccg acacgttgcc agacagtatc accatcgtct gttcgctgac
    24121 cggacctagc ccgacatgtg tcggagaccg tacagacaaa cccttgggtt tgggctttaa
    24181 tttcggctaa aaggaacccg ctaacgcatt ggactatcga ggaattaggc cggagcaacg
    24241 gctgtatttg atctaacgcg gagcgaccgc atgcgcgtcg cagtaatcgt tgtgttcgcg
    24301 tcaatgctga ccacaccgcc ggaagcttcg acgtcctgca tgagcaagac cgaagcgcgt
    24361 cagcaatttg gttcgtacat ctattggcat ggcccggagc attgttggga cgcgacaccg
    24421 acgcgtccct atggggttcg ccatcgcgtg caccaacaag aacccgaacc aacgccaccc
    24481 gccatggtcc cgatgccatc gcccgatcgg cgcagatcgg cgaacgcgat ggctatcgat
    24541 gaaccccaga tcgatctgat aaccgcaaca ccatgggtcg agcgctggac cgatatcacg
    24601 caggtcatgc ccccgcagtt ggtcgtcgcc atcccaaaac ccgagccgat ggtgacgccg
    24661 cacggcgtgg tgctagtgat cataaccatc atactgacgc ttgcgattgt cgaaattctg
    24721 ttcggcggga tgatcaacga gcgaatgcgc aagcggcgtc atcgcggatt cgcacgatga
    24781 cctgtccgaa gtgaggggca agacaatgac cgatgagcaa ctcgcagcca tgcacaggtt
    24841 acttgccgaa taccatcgca atctcgccaa ggaagcagtc ctcgacatcg tgcaacagta
    24901 tcacaccgat cttgcgcagc ggctggcgga tgaggcctca cagataccaa ggcgcacagc
    24961 gatcctggca cgccttcatg aacgcgaaca acaaaacatc gagtatggcg acgacggaag
    25021 ttgaagagga acgcgccatc aaactcactc acggatacga agagatggcg ccggaaagcg
    25081 tccgccagac ccgcacccgg atgggaccgg ccttcgtatc aatacgttgg atcatggcag
    25141 cggtatagcg cgcaccgagg ccgatcattt catgaaatgc ctggctgcgg tcaatggttc
    25201 gacatgcgcg acacctgcca aatgctggcg cacgttcaca acgcagagat tgagatcggc
    25261 gatggttaca gccgctggat tagccgttgc atcaatggct caaagaacag gcaactgtca
    25321 cgacgaaatc gtgccgcagt cgtcccttca ccctccggca ccgcgccgga aagcgccaaa
    25381 ggtctaccgg tttattcgat agaccacgaa gttggctcct ttatacggcc tttgccgagg
    25441 cattcctcgc atttcgcagg atagattttg cggcccggtt gcgttggctg cttgaccttc
    25501 tgcaaccctg tgccatcaca ggcggggcaa atagcttcgg tcaaaacttc acgctttttt
    25561 ttcataacga ccaatcatta aaacaagaca aaaagttgaa ttcatttgat aacggctagt
    25621 cgcaacagcg caacgaaact cgcttcttaa cctaaccggt aaacgttacc gccaccgagg
    25681 aactcgcgtt gtctcccaag aggccgcgcg agcgctgcag ttggctgttc gagggtcgga
    25741 tatccgggct gatcagacga ccctcatcga gatacccacg tcgtctagct cgctgttgtc
    25801 gctctcgccg atgcccgctt tatgtcgcat ttccgactca agtcagacag cgcgccaagt
    25861 acgaaaagtg ccaaataccg gaagtcgcta tttattcaat cacctcgtcg gcacgcgcga
    25921 gcagcatcag cggaatgtca agcctgaatg aacacgcggt ctgcagattg atgacgaact
    25981 cgaatctagt cgattgcaga accggtaggt ccgtgggttt cgcgccctta aggatgctaa
    26041 cgcaatcacc tgcggctttg gcaagttcct tggatcgaat ccccgcggcg acaacggcgc
    26101 gatcacaggt gatgggtcct tggtcgtcag cacagcactg agttttccgt ctcgaatatc
    26161 gaatccggtc gcgtgcgctc ggcagagggt cgctccaagc gactgggcag cttgcttgag
    26221 tgtcatgtga tgcgaatggt gatcgctcca cccaaccgcg aaccttcgcg tgccttctac
    26281 accgatcgtt cgcgagaacg ccctctccgc aatgctatcg tcgctcgctc tgatgctcta
    26341 ggcgatccag tcgtcgtgcc caaggatcga tccgcgacgc tattccggcg ttggaaacaa
    26401 acaacaattt gtcgtccgtc gaactctcat cccaaagagg cagtcctgct ccat
//
LOCUS       DASWGM010000512         4770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1997133,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000512 DASWGM010000000
VERSION     DASWGM010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997133"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /gene="lptD"
                     /locus_tag="VGA15_14850"
     CDS             <1..1711
                     /gene="lptD"
                     /locus_tag="VGA15_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX9589011.1"
                     /translation="STYGYAVETPFYWAIAPDYDATFTPRFTTRQGVLLQGEFRQRLI
                     NGSYQIRAYGIDQLDQKAFAGQPGDRQFRGGIETKGQFALNDKWVWGWDGVLLSDYFF
                     FSDYKLAQYRDPLGSFLSLPTEAISQLYLTGVGSRSYFDARTIYYLSYSGNQAQVPVI
                     HPVVDYSNVINHQVLGGEFSYKTNFTSLTRETAAFDPITTLANTNGLCLTASADPMAR
                     LPAQCLLRGMPGTYTRASVEAQWRRSFTDSAGEIWTPFAIVRADAIDASISNQPGVSN
                     FLPVGDTQALRVMPTVGLEYRYPFINVQPWGTTTIEPIGQVIIRPNESYAGKLPNEDA
                     QSMTFDASNLFAVDKFSGYDRVEGGGRANVGVQATTQFDRGGSVNVLFGQSYQLFGLN
                     SFAVADVTNTGVDSGLQRARSDYVGSVNYSPNRTYTFSVRSRLDEQTLNINRFEAEGR
                     ASFDRWSVALTYGNYAPQPDLGYLIRREGLLGSGSIKVAANWVVSGAARWDLEANKLN
                     QYIVGAGYVDDCFVLAANYVTSYQYSAGTTPPVLSHAFMLQIGLRTLANTSSSASGAG
                     GIQ"
     gene            1790..2728
                     /locus_tag="VGA15_14855"
     CDS             1790..2728
                     /locus_tag="VGA15_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX9589012.1"
                     /translation="MTTIRILHRPLILGCAAALILIGCGSPLHAQTVAVMVNGEPITN
                     YDIDQRGKLDFLTTHKPAVRQQVIDVLIDEKLKIREGKKFGIDPTASDVDQSFAGMST
                     RMRTTPELLAKSLESQGVRPDTLKARMKAEMVWTSLVRGRYKESLQVGEKDVAEAVQV
                     KGDDKDKQETESFEYQMRPIVLILPKGSAQAAIESRVKEAEALRGRVQSCDEANAFFK
                     TMPNAAIRETVTRTSADLPAAVRDGLDKTPIGHLTAPEVTKQGVEMAALCAKKPTTVD
                     TPRKKEIREKMYAEKYEAKSKAYLQEVRKAAMIEYR"
     gene            2732..3736
                     /gene="pdxA"
                     /locus_tag="VGA15_14860"
     CDS             2732..3736
                     /gene="pdxA"
                     /locus_tag="VGA15_14860"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX9589013.1"
                     /translation="MAKPLALTSGEPAGIGPDITIKAWLRRNELKLPAFYLLGDRDLL
                     GDRADRLGLKVRLADVAPEDACKAFADALPVVATGHRATARPGQPDDTSADAALASIR
                     QAVGDVIAGRASAVVTNPIAKSVLYRAGFRHPGHTEFLAELAATGGHTPQAVMMLWSP
                     ALAVVPVTIHISLREAIAQLSSDLIVTTVRIVAAALKARFGVAKPRLAVSGLNPHAGE
                     DGSLGTEDRTIVAPAVERLRGEGIEIRGPLPADTMFHDAARKTYDCAICMYHDQALIP
                     VKTLAFEDAVNVTLGLPFIRTSPDHGTAFDIAGTGRANPSSLIAALRLAARMAAAPPA
                     "
     gene            3733..4599
                     /gene="rsmA"
                     /locus_tag="VGA15_14865"
     CDS             3733..4599
                     /gene="rsmA"
                     /locus_tag="VGA15_14865"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX9589014.1"
                     /translation="MSAIDDLPPLREVIRQHALSARKSLGQNFLLDLNLTARIARAAG
                     PLADATIIEIGPGPGGLTRALLALGARRVIAVERDQRALAALEEISRRYPGRLEIICA
                     DAQYFDPRPLLGGGKAKIVANLPYNIATALLIDWLSIEPWPPWYDMMVLMFQREVAER
                     IVARENDEAYGRLGVLANWRAETKILFDISPAAFVPQPKVTSSVVRLVPRGTPEACDR
                     RALEQVAAAAFGQRRKMLRQSLKSLAVDPARLATAAHVDLTRRAETVPVSGFVAMARE
                     LTDIRNTRMNIA"
     gene            4612..>4770
                     /locus_tag="VGA15_14870"
     CDS             4612..>4770
                     /locus_tag="VGA15_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX9589015.1"
                     /translation="MALMRRQSLVKFDAPLCETIIETPKPLAKEVLVRIERCGLCHSD
                     LHIQDGYAD"
BASE COUNT          922 a         1503 c         1472 g          873 t
ORIGIN      
        1 ttcgacctat ggctatgccg ttgaaacacc gttttactgg gcgatcgctc ccgactacga
       61 cgcgaccttc accccacgct ttacaacccg gcagggcgtg ctgttgcagg gcgaattccg
      121 tcagcgcctg atcaacgggt catatcaaat ccgcgcctat ggcatcgacc agctggacca
      181 gaaggccttt gccggtcagc ccggcgatcg tcagttccgc ggcggcatcg aaaccaaggg
      241 acagttcgcg ctcaacgaca aatgggtgtg gggctgggac ggcgtgctgc tgtcggacta
      301 tttcttcttc tcggattaca agcttgccca gtacagggat ccgctggggt cgttcctgag
      361 cctgccgacg gaggcgattt cccagcttta tctgactggc gtcggcagtc gcagctactt
      421 cgacgcgcgc acgatctatt atctgagcta ttcgggcaat caggcccagg ttccggtgat
      481 tcacccggtc gtcgattatt cgaacgtgat caatcatcag gtccttggcg gagagttcag
      541 ctacaagacg aacttcacca gcctgacgcg ggaaaccgcg gcgttcgatc cgatcacgac
      601 attggcgaat accaatggcc tgtgtctgac ggcgtctgcc gatccaatgg cgcggctgcc
      661 ggcgcaatgc ctgcttcgcg gcatgccggg cacctacacg cgggcgtcag ttgaggcgca
      721 atggcggcgg tcgttcaccg attccgccgg cgaaatctgg acgccatttg cgatcgtccg
      781 cgccgatgcg atcgatgctt cgatctcgaa tcagccgggc gtttcgaatt tcctcccggt
      841 gggcgacacg caggcgctgc gcgtgatgcc cacggtcggt ctcgaatacc gctatccctt
      901 catcaacgtg cagccgtggg gaaccacgac gatcgagccg atcggacagg tcatcattcg
      961 tcccaacgaa tcctatgcgg gcaagcttcc caacgaagac gcgcagagca tgacgttcga
     1021 cgccagcaac ctgttcgcgg tcgacaaatt ctcgggctac gaccgcgtgg aaggcggcgg
     1081 ccgggccaat gtcggcgtgc aggcgaccac gcaattcgac cgcggcggct ccgtcaatgt
     1141 gctgtttggg cagtcctatc agctgttcgg cctgaattca ttcgcggtcg cggacgtgac
     1201 caacaccggt gtcgattccg gtctccagcg tgcgcgatcc gactatgttg gcagcgtcaa
     1261 ctattcaccc aacagaacct ataccttcag cgtgcgctcg cgcctggatg agcagacgct
     1321 gaacatcaac cgtttcgagg ctgaaggccg cgcgagcttc gaccgttggt cggtcgccct
     1381 gacgtacggc aattatgccc cgcagccgga cctcggatac ctgatccgtc gcgaaggatt
     1441 gctgggcagc ggctcgatca aggtggccgc gaactgggta gtctcggggg ccgcccgttg
     1501 ggatcttgaa gccaacaagc tcaatcaata catcgtcggc gcaggctatg tggacgactg
     1561 cttcgtgctg gcggcgaact acgtgacttc ctaccagtac tccgcaggca ccacgccgcc
     1621 cgtgctcagc catgcgttca tgttgcagat cggcctgcgg acgctcgcca acacgtcctc
     1681 ctcggccagt ggggccggcg gcatccaata gggccggctt tgacgctgcc ggagggttgt
     1741 tcctgcgtca cggatccagt tcgaatcggt tgatatagcg aatatgatca tgacgaccat
     1801 taggatctta catcgccctc tgattctggg ttgcgccgca gcgctgatct tgatcggttg
     1861 tggttcgccg ttgcatgcgc agaccgtcgc cgtcatggtc aatggcgaac cgatcaccaa
     1921 ctacgatatc gatcagcgcg gcaagctgga cttcctgacg acgcacaagc ccgccgtccg
     1981 ccagcaggtg atcgacgtgt tgatcgacga aaaattaaaa atcagggaag gcaagaagtt
     2041 tggcatcgac cctaccgctt cggatgtcga ccagtcgttc gccggcatga gcacacggat
     2101 gcgcacaacg cccgaactgc tcgccaaatc cctcgaaagc cagggcgttc ggcccgatac
     2161 gctgaaggct cgcatgaagg ccgagatggt ttggaccagt ctggtccgcg ggcgatacaa
     2221 ggagagcctg caggtcggtg agaaggacgt cgccgaggcc gtccaggtca agggcgacga
     2281 taaagacaaa caggagaccg agagcttcga gtatcagatg cgtccgatcg ttttgatcct
     2341 cccgaaagga tccgcacagg ccgccatcga atccagggtc aaggaggccg aagccttgcg
     2401 cggccgtgtg cagagctgcg acgaggccaa cgctttcttc aaaaccatgc caaatgccgc
     2461 gatccgcgag accgtgacca gaacttccgc cgatctgccg gccgctgttc gtgacggact
     2521 cgacaagact ccgatcggcc atttgactgc gcccgaggtg acaaaacagg gcgtggaaat
     2581 ggctgcgcta tgcgccaaga agccgaccac cgtcgacacg ccgagaaaga aggaaatccg
     2641 cgagaagatg tatgccgaga aatacgaagc gaagtcgaaa gcgtatctgc aggaagtccg
     2701 caaagccgcg atgatcgaat atcgttgatg catggcaaag cccctcgcgc tgacatccgg
     2761 cgaacccgcg ggtatcgggc ctgatatcac catcaaagcg tggctgcgcc gcaacgagtt
     2821 gaagctccct gcgttttacc tgctcggcga tcgcgacttg ctcggcgacc gggcggacag
     2881 gctcggactg aaggtcaggc tcgccgacgt tgcccccgag gatgcctgca aagcattcgc
     2941 ggatgcttta ccggtcgtgg ccactggcca cagggcgacc gcacggccag gccagccgga
     3001 cgacaccagc gcggacgccg ccctggcctc cattcgccag gcggtcggcg acgtgatcgc
     3061 cggccgggcc agcgctgtcg tcaccaatcc gatcgccaag agcgttctct accgcgctgg
     3121 tttccggcat cccggccata ccgaatttct cgccgagctc gccgccaccg gcggccacac
     3181 gccgcaggcc gtgatgatgc tgtggtcgcc tgccctcgcc gtggtgccgg taacgatcca
     3241 catttcgctg cgcgaggcga tcgcgcagct gtcgagcgac ctgatcgtta cgacggttcg
     3301 catcgtggcc gcggcgttga aggcgcgctt cggcgtcgcc aagccgcgcc tcgcggtgtc
     3361 cggtctcaat ccgcatgccg gcgaagacgg ttcgctcggc acagaagacc gcacgatcgt
     3421 tgcaccagcg gtcgaaaggc tgcgcggcga aggcatcgag atcagaggac cgctgccggc
     3481 ggacaccatg ttccacgacg cggcgcgcaa aacctatgac tgcgccatct gcatgtatca
     3541 cgatcaggcg ctgatcccgg tcaagacgct ggcatttgaa gacgccgtca atgtcacatt
     3601 ggggttgccg ttcattcgca cgtcgccgga ccatggcacg gcgttcgaca ttgccggcac
     3661 cggacgggcc aatccgtcga gcctgatcgc ggctctgcgg cttgccgccc ggatggcggc
     3721 agcgccaccg gcatgagcgc gatcgatgac ctcccgccgc ttcgcgaggt catccgccag
     3781 catgccctgt cggctcgcaa atcgctcggt cagaattttc tgctggacct caacctgacc
     3841 gcgcggatcg cgcgggccgc gggccctctc gcagatgcga cgattatcga aatcggcccc
     3901 ggccccggcg ggctgacccg cgcgttgctg gcgctcggcg cgcggcgcgt catcgccgtg
     3961 gagcgcgacc agcgcgcact ggcggcgctc gaggagattt caaggcgata tcccggacgg
     4021 ctcgagatta tctgcgccga cgcgcagtat ttcgacccac ggccgctgct cggcggcggc
     4081 aaagcgaaaa tcgtcgccaa tctgccctac aatatcgcaa ccgcgctgtt gatcgactgg
     4141 ctgtcgatcg agccgtggcc gccctggtac gacatgatgg tgctgatgtt ccagcgcgaa
     4201 gtggccgagc gaattgtcgc ccgcgagaat gacgaagcct acggacggct gggcgtgctg
     4261 gcgaactggc gcgccgagac caagatcctg ttcgacattt ctcccgcggc attcgtaccg
     4321 cagccgaagg tcacctcgtc ggtcgtgcga ttggtgccgc gcggcacgcc ggaagcgtgc
     4381 gaccgcaggg cgctggaaca ggttgcggcg gcagcgttcg gccagcgtcg aaagatgctg
     4441 cggcaaagcc tcaaatcatt ggccgtcgac cctgcccggc tggcgacggc agcgcatgtc
     4501 gacttgacgc ggcgcgccga gaccgttccg gtgagcggct ttgttgccat ggcccgcgaa
     4561 ttgaccgata tacgaaacac aagaatgaac atcgcttaag gagagaatgc catggcgctc
     4621 atgcgtcgtc agtcgctggt gaaattcgat gcgccgctgt gcgaaaccat catcgagacg
     4681 ccgaagccgt tggccaagga agtcctcgtg cgtatcgagc gttgtgggtt gtgccattcc
     4741 gacctgcata tccaggacgg ctacgccgac
//
LOCUS       DASWGM010000513        15079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1560213,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000513 DASWGM010000000
VERSION     DASWGM010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15079
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560213"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VGA15_14875"
     CDS             <1..406
                     /locus_tag="VGA15_14875"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973425.1"
                     /note="Catalyzes the cycloisomerization of
                     cis,cis-muconate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEX9589016.1"
                     /translation="ATGPWHLEWVSLPESFLLTASSLANAKFMLAGLVVHERRMRENL
                     DLTHGLIVAEAVMMAAAPKLGRQQAHDVIYEACRKAIEGGGQLADTLADMPQIIDALG
                     SVESIRHHCDPANYLGLSGQMVDRALAARPHG"
     gene            582..1985
                     /locus_tag="VGA15_14880"
     CDS             582..1985
                     /locus_tag="VGA15_14880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9589017.1"
                     /translation="MRQDPVRAGWRSLLRREWIPTLAVLLGGVLLQSMNVLMLTTVLP
                     SIVGELGGVAMLSWPTTAFLASSIVAASCAGMLAAVVGARAAYCAGVTIFGLGALLCS
                     LATTMGWIVAGRLIQGFGGGLEAAAAYVAVRATFPEAVWSRTIALMSTSWSMSVLLGP
                     LVGGMFARFGNWRSAFVVTATVAGILAVSAFVILPPRTSGRQTPAAGMPAGRVALICF
                     AIAGMSSASIVQSPLAKAGLIVFAIGTLVAMLRLDRVAAAPLLPSDAFSLSTPTGMGL
                     WLALLLCITFSPLQIYVPIFLQHLHGLDPLAAGFTVASGSLGWTIASLVTAGASGAWP
                     DRLMSTGPAMMGVSLMAIALLLPSSAPALALVPAIVALGMGIGQCWPFVAHRIMSGSK
                     AGDEVVAASSVPTVQQTGFALGAALAGVVANASGFSAGVADDSMMQVAFWVPASFVVP
                     AAIACLASLRLRHLRKP"
     gene            complement(2153..3160)
                     /locus_tag="VGA15_14885"
     CDS             complement(2153..3160)
                     /locus_tag="VGA15_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9589018.1"
                     /translation="MRYTLRQIEYFIATAETGSITLASERVNISQPSISTAIAHLEEE
                     LETQLFVRRHAQGLSLTSAGRVLLVEAKRLVEQAEHMYSIASEVGERVRGQLSLGCLV
                     TLAAMVMPELSHSFTSAYAETRIVHMVGDHEQLLERLQHAEIDVAITYNLLIPDGFEF
                     LPLASLPPHAVVSEGSPLARDSAVSLQELVSERLILLDLPISRDYLLGLFMKEGLEPH
                     VATRSTHPDVIRTMVANGYGYTLANVRPRCEVALDGRRVVRVRLSGEHKPMTIGVLTL
                     KQARKYRLVEFFQQHCRRFVSDAYIPGMVAPALERRTFLGQTATEVTKPGDPIDRTPP
                     V"
     gene            complement(3240..3911)
                     /locus_tag="VGA15_14890"
     CDS             complement(3240..3911)
                     /locus_tag="VGA15_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX9589019.1"
                     /translation="MTFSLTARCTKTGMFGIAISSSSPAVAARCVHARPMVGAIASQN
                     ITDPQLGQIGLDLLARGFNAEEVRAALVASGRFIIYRQLAIVDSTGGTASYSGSGTLG
                     IHATCTGDGAVAAGNLLVTDQVPAAMIAAYRAAPGALAERLLSGLRAGLDQGGEAGPV
                     HSAGLYVVRDISWPIVDLRIDWSDRPIAALHELWAVYAPQIEDYVRRARQPVDAPGFG
                     VAGEA"
     gene            complement(3921..4340)
                     /locus_tag="VGA15_14895"
     CDS             complement(3921..4340)
                     /locus_tag="VGA15_14895"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9589020.1"
                     /translation="MAHTRIRKFNTKDTYPEQSLDNDLCQTVVAKGSMVFVRGQVGQD
                     LDSSKSVAIGDPTGQAEQAMANIKTLLEEAGAKLEHICKITIYIIDPRYREPVYRTVG
                     RWLKGVFPVSTGLVVSALARPEWLVEIDAIAVIPDER"
     gene            complement(4364..4735)
                     /locus_tag="VGA15_14900"
     CDS             complement(4364..4735)
                     /locus_tag="VGA15_14900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9589021.1"
                     /translation="MRWRSGRTLALADLVAEPLIELGSCPSATYCRTLFEAEGLSPRI
                     GITAISPDMVRRLVAHGFGYSLLSAKSADVLAQDALALAAIPLSKPVVPCRLLLGRRT
                     RDVEFHPLAQTFRDICRSYFA"
     gene            complement(4674..5537)
                     /locus_tag="VGA15_14905"
     CDS             complement(4674..5537)
                     /locus_tag="VGA15_14905"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEX9589022.1"
                     /translation="MPEIGVRKNVPDIRAQFIDGMSRAATCVSVVTTDGPAGRFGVTV
                     SAMSSISADGEHPTVLICVHHQSRSAAAIIENGTFCINVLRDDQSSIADCFADRIRRA
                     DGDKFGCARWVRESTGAPRLADPLAAFDCRVLSTERVGTHHIFIGAVETVVTAGPGAA
                     LIYADRAYGTTQRIEAPRPKTAGARPQLTIGCNQVFAPYVMPELLTRMSASHPDGVAS
                     LVDGDQRRILESLKSGETDIALLSDFELGAGIAATMLAEWQPYVLLPTAHALAERAHP
                     RPRRSRRRAAD"
     gene            complement(5579..7099)
                     /locus_tag="VGA15_14910"
     CDS             complement(5579..7099)
                     /locus_tag="VGA15_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HEX9589023.1"
                     /translation="MIRTGEDYKASIRESRDVYMNGERVKDVTAHPMFKPLVDIRARI
                     YDLQHDPLTRHLMTYQEDGEIHAINNKLPISQQDWWDKRRATDTVLEDIGGVVTRVGD
                     ETVGEMWSLFDGQDVLNEVDPQFSKNIREHIFKVLHTDPFHVSANTDPKGDRSKAPQD
                     QDPDMLLHVVRETDSGIIVRGAKYETAAAYANQAFTKPTIANWGDSAYSDYAVGFICD
                     LSTPGLKFICRTGFSGRAPLDDYPLSNRFDEIDTLVIFDDVLIPWENVLFYRHTKAAV
                     FIRATLHRYSAFAFVQRSLKFADMMIGAALFNVRQTGLEKQQAVQEKLSQLAVYREGI
                     NAHLTAAIALPERSPGGLMMPNQSLLYTGRVLAGSQLPQMMHLARELCGGQICVTPDK
                     ASFDNPETKPWLEKFYTINDDWVADDRRRLLAFARDLLNSDYAGHRLTFQLFAQSPPF
                     ANLAAVYRNFEWSGPLDFVRKSAGLSEKTLGKTPRSAGDRAVNQWFAVRRIQDAAE"
     gene            complement(7432..8160)
                     /locus_tag="VGA15_14915"
     CDS             complement(7432..8160)
                     /locus_tag="VGA15_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase and adducin N-terminal
                     domain-containing protein"
                     /protein_id="HEX9589024.1"
                     /translation="MTNEHELRVDLAAAFRIIAKLGMHEAVANHFSAAVSPDGRKFLM
                     NPKWRHFSRIKASDLVLFDADTPDDAKERPDVDPTAWAIHGQMHKLLPQARVVMHLHP
                     PFTTTIATLADPQVRPIDQNTARYFNRLAVDGSYGGMADSAQEGARLARLLGNKSRLM
                     MGNHGVLITANTIGEAFDDMYTIERACQILVQAYSTGQPLNVLPDDIAERTAQDWEQI
                     TDFSIAHFEEMKRILDAEDPSYSH"
     gene            8819..9835
                     /locus_tag="VGA15_14920"
     CDS             8819..9835
                     /locus_tag="VGA15_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589025.1"
                     /translation="MALQPLELKVYSGELLALLGPSGSGKTTLLQVICGLIEPTGGRL
                     IIDGRDETDKPVNKRDIGVVFQNYALFPHLTVRENVSFPLQMRKIAAADLREKVEATL
                     AMVGLSDFGSRFPRELSGGQQQRVALARCLVYQPSLILMDESLSALDRKLRESMQIEI
                     KRLHRETGATIIFVTHDQEEALALADRICLMNEGRIEQLGTPEDMYEQPRSAFVADFI
                     GISNVMRGRVQSGGMIRTDDGSLPLPDGCEARAGEDGALIVRPEKMLLCAIDEAFVSG
                     RVEETIYAGSETKLLVRLPSGTLITVRRQAGLPGVAIGEHVSIRWDKQQARLLETGLV
                     SMIE"
     gene            9953..11032
                     /locus_tag="VGA15_14925"
     CDS             9953..11032
                     /locus_tag="VGA15_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589026.1"
                     /translation="MSTFETITRRKLLKAASVAATGVAMPVVWTKRARAAEEITVADV
                     GGATAPALRAGFYDPFEKETGIRVINVAHESDPVTQFKLVVDTGSKIWDVCMVTPDDV
                     ARLTADKNYLESLDIAPSEDQDLIPGALKPNWLGFSVYAIVMAYRSDVYKTEAPKSWA
                     DFWNTTKFPGRRGLYRSPKGVLEAALLADGVAPKDLYPLDVDRAFAVLDKVRSQVAVW
                     WTSGAQNTQILQSGEIDMSDTWSARAFAAIDSGAPVNIVWNGLYSIDGWSIPLGTPKL
                     KQARAFVRFCMHPERQGAYSSMVANGPSNLKAYNFIAPARAKVLPTYPENLKGLTERD
                     FAFWGKNFAAVSDRFQEWLLMGGHR"
     gene            11112..12476
                     /locus_tag="VGA15_14930"
     CDS             11112..12476
                     /locus_tag="VGA15_14930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9589027.1"
                     /translation="MNKKKQMTIGALIHANGSHSASWLLQEAHPHASTDIDYYRGIAQ
                     LAERGKFDFFFIADTPAARTENLRVWSRSPLFMNVLEPITLLSAVAGATTHIGLGATA
                     STSFFEPYNLARQFASLDHISHGRAAWNVVTSANDYAARNFGLDRLPPHGERYAKARE
                     FVEVVEALWDTWEDGAFVYDRDACLSFLPEKFHGLEHKGKYFTVQGALNIERSPQGRP
                     VIIQAGASDTGRDFAAETAEVVFGSGASLDEAKAFYRDLKGRMGKFGRQPDELKIATG
                     ISVVIGESEQEARDKLQSWQELIHPDVGVLRLGQDLETDLSDLPLDEPIPEHRIPQSS
                     NFHKAYFDQIVGMISQRMTLREMSMRYNRSKATFCGTALQVADHMEAWMAADACDGFM
                     ISFLALPSSLREFVDTVVPELQRRGLFRTDYAGRTLRDHLGLKHPENRYVSQARAASI
                     AKRA"
     gene            12517..14301
                     /locus_tag="VGA15_14935"
     CDS             12517..14301
                     /locus_tag="VGA15_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9589028.1"
                     /translation="MTDSVSLATVALARQRKLRIEPLWLALPGVVFLVLFLLLPTAQM
                     LSRSLFDKVTGALTLAAFARIWNGGPYLAVLSTTFSVAFWTTLLCLTLSYPLAYWLCC
                     KSPRQQRIAMLFVLLPFWTSALIKNFSWLVLLGRNGIMAKIMASAGMTGGDQLLFSRT
                     TVVFAMTHTLLPLAVVTMLPVMNQIDRRLPMAAATLGASGAQAFWQIFYQLSMRGVAA
                     AGLLVFVASLGFFITPALLGGPRETMIGQLIILQINELQNWQLGSALAAILLVSAIAM
                     CMVYDRIFGLSSVSEGSERQTSPGGLARLAGLAAARFAGLVFGAIEEFWKRNIRGLRG
                     SAILSIYAWVVIAVLLVPIIAFVPMAFTRSTFLSFPPQGFSLQWFEQFMASPLWLGAM
                     IRSFGIGLATAAITVVIASLAALGLARSRSRLAGAVFLLFLTPMVVPSIVIAIALFYL
                     FAQVSLVATNLGIIIGHTVIATPVVFVILLATFKGHDWRLDAAASTLGASRAQTFWRV
                     TLPLVKGGFAVGFVTGFLQSFEELTVALFVGGGLKTTLPKQMWDGILLQVSPVIAAAS
                     VVVLVVVILMFAIMECVQERSAAARRYS"
     gene            14717..>15079
                     /locus_tag="VGA15_14940"
     CDS             14717..>15079
                     /locus_tag="VGA15_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9589029.1"
                     /translation="MIQSEDSKISEDRREALRYALAVGSGAALAAAVASPASAQATPD
                     NTLDRIRANKALRIAVLPGELPYFNKDLATGNWSGFSIEMANDIAKLLDVKLEYTEST
                     YGNSILDLQSNKIDLGFAL"
BASE COUNT         2742 a         4484 c         4767 g         3086 t
ORIGIN      
        1 tgcaacaggt ccgtggcatc tcgaatgggt ttcgttgccg gaaagttttc tcctgaccgc
       61 gtcctcgctg gctaacgcca agttcatgct cgcagggctc gtcgttcacg agcggcggat
      121 gcgggaaaat ctcgatctga cccatggcct catcgttgcc gaagccgtga tgatggcggc
      181 ggctcccaag ctgggacgtc agcaggctca cgacgtcatt tacgaggcat gccgcaaggc
      241 gatcgaggga ggcggccagc tcgccgatac cctggctgac atgccccaga ttatcgatgc
      301 gctcggcagc gtggagtcga ttcgccatca ttgcgatccc gccaattatc tggggctttc
      361 aggccagatg gtcgatcgcg ccctggccgc gcgaccgcac ggatgaaaga tagagcggtg
      421 tgataggcgt ccagccgatc agatcatcag agatccaatt ccaccggcgg aatagctcac
      481 ggccgggcct gctttaactt ggaaagcaaa tactatctaa aataacccgg ctgtcgccga
      541 caaccctaag gcgccggagc cccggaaggg agacaacagg catgcggcag gacccggtgc
      601 gcgcgggatg gcgatcgctg ctcaggcggg aatggatacc gacgctcgcc gtcctgttgg
      661 gcggcgtgct gctgcaatcg atgaatgtgc tgatgctgac gaccgtgttg ccgtccatcg
      721 tcggcgaact cggtggtgtc gccatgttga gttggccgac gaccgcgttt ctcgcatcat
      781 ccattgtcgc cgcgagctgt gccggcatgc tcgcggctgt ggtcggtgcc cgggcggcct
      841 attgcgcggg ggtaacgata ttcggtcttg gcgcgttgct ttgctcgctc gcgacgacca
      901 tgggctggat cgtcgccggc cgcctgatcc agggattcgg tggcggtttg gaggcggccg
      961 cagcttatgt ggcggtgcga gccactttcc cggaagcggt ctggtcacgg acgattgcat
     1021 tgatgtcgac cagttggagc atgtccgtct tgctggggcc gctggtcggc ggaatgttcg
     1081 cccgcttcgg caattggcgc agcgcgttcg tcgtgaccgc gaccgtcgcc ggcatattgg
     1141 ccgtgagcgc ctttgtcatc ttgccgccga ggacatcggg acgccagacg cccgctgcag
     1201 gcatgcccgc tggacgtgtc gcgttgatct gcttcgcaat tgccggaatg tcttcagcgt
     1261 cgatcgtcca atcaccgctt gctaaagccg gtctgatcgt cttcgcgatc ggcacgctgg
     1321 tggcgatgct gcggcttgat cgggtcgccg cggcgccgct gctgccgagc gatgcgttct
     1381 cgttgtccac gccaaccggg atggggttgt ggttggcgct gctgctttgc atcactttca
     1441 gcccgttgca gatctacgtg cccatcttcc tccagcacct gcacggcctg gatccactgg
     1501 cggccggctt cacggtcgca agcggctcac tgggatggac gatcgcttcg cttgtcacgg
     1561 ccggcgcatc tggggcatgg ccggaccgcc tgatgtcgac cggaccggcc atgatgggcg
     1621 ttagcctgat ggccatcgcc ttgcttctcc caagctctgc gcctgctctg gccttggtcc
     1681 cggccattgt cgcgctgggg atgggcatcg gccaatgttg gccctttgtc gcgcaccgga
     1741 tcatgagcgg ctcaaaagcg ggtgatgagg tcgtcgcagc ctcctcggtc ccgaccgtcc
     1801 agcagacggg attcgccctg ggcgcggcac tcgcgggcgt ggttgcaaac gccagtggct
     1861 tctccgccgg agttgcggat gacagcatga tgcaagtggc cttctgggtg ccggcgagct
     1921 tcgtcgtgcc ggcggcgatc gcctgcctgg cgagcctgcg cctgcgccat ctacggaagc
     1981 cctaaggcta actcgtcgcg acctgcacgt gcgtcgagcg cattcagatc ttccttcgtg
     2041 agcctgaccg atatggctcc gaggttgcgt tatgtgcgga acgccaccga aagcgtgcac
     2101 gtacgatcag gcgtgagcga gcacctcatg aattcgctgc atggcgatct agtcaaacag
     2161 gaggtgtacg gtcgatcgga tcgccaggct tcgttacctc ggtggcggtt tgacccaaga
     2221 aagtccgacg ctccagcgcc ggcgcgacca tccctggtat gtaggcgtcg gagacgaatc
     2281 tgcggcaatg ctgctggaag aattccacaa ggcgatactt tctcgcctgc ttcaaggtca
     2341 agactccgat cgtcatcggc ttgtgctcgc ccgacaggcg aactctgaca acacgccgcc
     2401 catccagcgc gacctcgcac ctcgggcgca cgttcgcaag cgtatagcca tagccattgg
     2461 caaccatggt ccggatcacg tccggatgcg tcgagcgcgt cgccacatgg ggctcaagtc
     2521 cctctttcat gaataagccc agaagataat cccggcttat cggtagatcg aggaggatga
     2581 gccgttcgct gacgagttcc tgcaagctga cggcagaatc gcgtgccagc ggactgccct
     2641 cgctgactac cgcgtgcggc ggaaggctcg ccagcggcag gaactcgaag ccatcgggga
     2701 tcagcaggtt gtaggtgatc gcgacatcga tttcagcatg ctgaagccgt tccagcaatt
     2761 gttcatggtc gccgaccatg tggacgatgc gtgtttcggc ataggccgat gtgaacgaat
     2821 gcgaaagctc cggcatcacc attgccgcca gtgtcaccag gcaacccagc gacagttgcc
     2881 cgcgaacccg ttctcccact tcggaggcga tggaatacat gtgctccgcc tgctcgacga
     2941 gccgcttggc ttcgaccaga agcacacggc cggcggaggt gagcgagagc ccctgggcgt
     3001 gacgccgcac gaacagctgc gtctcgagtt cttcttccag atgggcgatc gcggtcgata
     3061 tcgatggctg ggatatattg acccgttccg acgccagagt gatgcttccg gtttcggccg
     3121 tcgcgatgaa atactcaatc tggcgaagcg tatatctcat gcgtcgattc ccagagcgaa
     3181 gagagacgat ccggaatcga acgtggtttt ccggtccgca tggcgagagc gcaactgtac
     3241 tacgcctcgc ccgccacccc gaaaccggga gcgtcgacgg gctgccgcgc gcgccgaacg
     3301 tagtcttcaa tctgcggcgc gtaaacggcc cacagttcat gcagtgctgc gatcgggcgg
     3361 tcgctccaat cgatccgcaa atccacgatc ggccaggaga tatcccgcac cacatagagg
     3421 ccggcagaat gaaccggccc ggcctctccg ccctgatcga ggccggccct taggcccgac
     3481 aacagccgtt ccgccagcgc gccgggcgcg gctcgatagg ccgcgatcat ggcggcagga
     3541 acttggtcgg tcacaagaag attgccggca gccaccgcgc cgtcgccggt gcaggtcgca
     3601 tggatgccga gcgtgccgct gccggaatag gatgcggtcc cgccggtcga gtccacgatg
     3661 gcgagctggc ggtagataat gaagcggccg gatgcaacga gcgcggcgcg cacctcttcg
     3721 gcattgaagc ctcgcgccag gagatcgagg ccgatctggc ccaattgcgg gtcggtgatg
     3781 ttctggctgg cgattgcgcc gaccatgggc cgggcatgca cgcagcgcgc cgcaaccgcg
     3841 ggcgaggagg aggagatcgc gatcccgaac atgccggttt tggtgcagcg agcggtcagg
     3901 gaaaaagtca tgccgccctc tcagcgttca tcggggatca cggcgatggc gtcgatttcc
     3961 accagccatt ccgggcgcgc gagcgctgac accaccagtc ccgtcgacac cggaaacacg
     4021 cccttgagcc acctgccgac cgtgcgatag accggctcgc ggtagcgcgg atcgatgatg
     4081 tagatcgtga tcttgcagat gtgttccagc tttgctccgg cctcttcgag aagcgtcttg
     4141 atgttggcca tcgcttgttc ggcctggcca gtcggatcgc cgatcgcaac gcttttcgag
     4201 ctgtcgaggt cctggccgac ctggccccga acgaacacca tgctgccttt cgctaccacg
     4261 gtctggcaca aatcgttatc gaggctctgt tcgggatagg tgtccttggt attgaatttc
     4321 cggatgcggg tgtgagccat gcacaatggt ccttgctgga agttcaggcg aaataggatc
     4381 ggcagatatc gcggaaggtc tgggccagcg gatggaattc cacatctctg gttcggcggc
     4441 caagaagcag ccggcagggt acgacgggct tagaaagagg gatagcggcc agcgccagcg
     4501 cgtcttgcgc cagcacgtcc gccgacttcg ccgacagaag gctatatccg aaaccgtgag
     4561 ccacaagcct tcgcaccatg tcaggagata tggcggttat tccaatccgt ggcgacaatc
     4621 cctccgcctc gaacaaggtt cggcaatagg tggcgctcgg acatgaaccg agttcaatca
     4681 gcggctcggc gacgagatcg gcgagggcga gggtgcgccc gctccgccag cgcatgtgcg
     4741 gttggcagca ggacgtaagg ttgccattcg gccagcatcg tggccgcgat tccagctccg
     4801 agttcgaaat cagacagcag cgcgatgtcg gtttcgcccg atttcaagct ttcaaggatg
     4861 cggcgctggt cgccgtccac cagactggcg acgccgtccg gatgcgaggc cgacatgcgc
     4921 gtcagaagct cgggcatcac gtagggcgcg aaaacctgat tgcatccgat tgtcagttgc
     4981 ggcctggcac cagcggtctt cgggcgcggc gcctctatcc gctgcgtcgt gccatacgcc
     5041 cggtcggcat aaatcagcgc ggcgcccggg cctgcggtga caacggtttc caccgcgccg
     5101 atgaagatat gatgggtgcc gacccgttcc gtcgacagga cgcggcagtc gaacgccgcg
     5161 agcggatcgg ccaggcgtgg tgcacccgtg ctttcccgaa cccagcgagc gcagccgaat
     5221 ttgtcgccgt cggcccggcg aatccggtcg gcgaagcagt cggcgatgct ggattggtcg
     5281 tcgcgcagga cgttgataca gaacgtgccg ttctcgatga ttgcggcagc cgatcggctt
     5341 tggtggtgga cgcagattag caccgtcggg tgctctccgt cggccgatat cgacgacatc
     5401 gcggacaccg taactccgaa tcggccggcg gggccgtccg tcgtcaccac gctgacgcaa
     5461 gttgccgcac ggctcatgcc gtcgatgaac tgcgcgcgga tatccggcac gttctttcga
     5521 acgccgatct ctggcatgga gtgctcctgt ctccccacaa gggcgtcaga tctgtgcgct
     5581 attcggcagc gtcctggatg cgcctgacgg cgaaccactg attgacggcg cgatcgccgg
     5641 cggatcgcgg cgttttgccg agcgtctttt cggacaggcc ggcggatttg cggacgaagt
     5701 cgagcggtcc gctccattcg aagttccggt agaccgccgc gaggttggcg aaggggggag
     5761 actgcgcgaa cagctggaag gtgagccgat ggccggcata gtccgagttc aacagatcgc
     5821 gcgcaaaggc gagcaggcgc cgccggtcat cggcaaccca gtcatcgttg atggtgtaga
     5881 atttttccag ccacggcttg gtttcggggt tgtcgaacga cgctttatcg ggcgtgacgc
     5941 agatctgacc gccgcacagc tcccgcgcca gatgcatcat ctggggcagc tgcgagccgg
     6001 cgagcacccg cccggtatag agcagggact ggttgggcat catgagaccg ccagggctgc
     6061 gttcgggcaa ggcgatggcc gccgtcaggt gggcgttgat gccctcgcga tagaccgcga
     6121 gctgggagag tttttcctgc acggcctgct gcttttccag gccggtctgg cggacgttga
     6181 acagcgccgc gccgatcatc atatcggcga atttcaggct gcgctggacg aaggcaaagg
     6241 ccgaatagcg atgcaacgtg gcgcggatga acacggcggc tttggtgtgc cgatagaaca
     6301 gcacgttttc ccaggggatc aggacatcgt cgaagataac cagggtatcg atttcgtcga
     6361 atctgttgga caacggataa tcatcgagag gggcgcggcc tgaaaagccg gtgcggcaga
     6421 tgaatttgag tccgggcgtg ctgagatcgc agatgaaccc caccgcatag tcggaatagg
     6481 cggaatcgcc ccagttggcg atcgtcggtt tggtgaaggc ctggttcgca taggctgcag
     6541 cggtttcgta tttcgcaccg cgcacgataa tcccgctgtc cgtctcccgg acgacatgca
     6601 ggagcatgtc gggatcctgg tcctgcggcg ccttcgagcg atcgcccttg ggatcggtgt
     6661 tcgccgagac atggaacggg tctgtatgga gcaccttgaa gatatgttcg cggatattct
     6721 tcgaaaattg cggatcaacc tcgttgagca cgtcctgtcc atcgaacaac gaccacattt
     6781 ccccgacggt ctcgtccccg acgcgcgtca ccacgccgcc gatatcctca aggacggtgt
     6841 cggtcgccct gcgtttgtcc caccaatcct gctgactgat ggggagcttg ttgttgatcg
     6901 catgaatctc gccgtcctcc tgataggtca tcagatgacg ggtcagcgga tcgtgctgca
     6961 gatcgtagat gcgagcgcgg atatcgacca gcggcttgaa cattggatgc gcggtgacgt
     7021 ccttgacgcg ctcgccattc atgtagacat cgcggctttc ccgaatggag gctttgtagt
     7081 cttcgccggt tcgtatcatt tccgctcctc tgcagttttc cttgggggca agacctcttg
     7141 gcgactattg cgtattgcct tttcgttgaa aaataggact ttccggcgct ttgcttagcc
     7201 gcgtccgatg caccggtgtt gtgaccgaag cgggcgcccg cgcggcgacg agaagctgca
     7261 cgcgaagatg acggtcatag ccgcctgcaa gcgggccgcc gctggaatat cccctcagca
     7321 ctcgtcaggc ccgagacgga tggagacatc tagcacatgg tgtcgccgcg gccgcaacca
     7381 cggctgtcac tctggagtct gatatcgaaa gctttcgtcg atatcgtgcg ctcagtgcga
     7441 ataggaagga tcttccgcgt ccagaatgcg cttcatctcc tcgaaatgcg cgatcgagaa
     7501 atccgtgatc tgttcccagt cctgcgcggt cctctcggcg atgtcgtcgg gcagcacatt
     7561 gaggggctgg ccggtcgaat aagcctgcac cagtatctgg caggcgcgct cgatcgtgta
     7621 catgtcgtcg aaagcctcgc cgatcgtgtt cgcggtgatg agaacgccgt gattgcccat
     7681 catcagccgc gatttgttgc cgagcagcct ggcgaggcgg gcgccttcct gcgcgctgtc
     7741 agccattccc ccataactgc catcgacagc aagccggttg aagtagcggg cggtgttctg
     7801 gtcgatcggt ctgacctgcg ggtcggcaag cgtcgcaatc gtggtcgtga agggcggatg
     7861 aagatgcatg acgacccgtg cctgcggcaa cagtttgtgc atctggccgt ggatcgccca
     7921 tgcggtcgga tcgacgtccg gccgctcttt cgcgtcgtcc ggggtgtctg catcgaacag
     7981 gacgaggtcg ctcgccttga tgcgcgagaa gtggcgccat ttgggattca tcaggaattt
     8041 gcgcccgtcc ggcgacaccg cggcgctgaa atgattggcg acggcttcgt gcattcccag
     8101 cttggcgatg atgcggaagg cggcggcgag gtcgacgcga agctcgtgct cattcgtcat
     8161 ggaagtcatt tgatccattt gccgtccggg aaaccgtccg aagctggata acggtcgcaa
     8221 aacgccaccg gacagttggc ttgtttaagc ggcctcggcg gattgtaaaa tatgaaaatc
     8281 atggcgactg gttagccgga gactatgacg agaccattgc cagggcagat gctcggcccc
     8341 cgcggttttc gattctattg tcggcaatct ggcgggcagc cgaggcatgc aggtcgccgc
     8401 cgccgccgat atgccaaggc agcggccgtg ctccgatgct ctcgacacag tgcccaagta
     8461 gtgctcatcc ggcacaataa ttgcgtcgcg ccttgatcga accatgcctt cgattaggcg
     8521 gggtctaatc aaagggctcg gatttcgtaa tttacacctc tcggcccgcg ccgcttactc
     8581 tccagaggcg tgcttttgta ggactgcgat gcaagtgatc agtgccctct cgtcccttcg
     8641 aacccgccgt gcggcggcgt ttcgattgcc gtgggtgttt acgcgaaacg aagaggagca
     8701 tcaggccgcc gtggacaggc tggagaccag gtcggccgag gtgacttcct caccagggca
     8761 agagcggtct cggatcaaac tgcatgcgca gggcctgtcc aaggcgtatg gggcctcgat
     8821 ggcgctgcag cctctggaat taaaggtcta ttccggagaa ttgctggcgc ttttggggcc
     8881 ttccgggtca ggaaagacca cgctgttgca agtcatctgc ggtctgattg aaccgacggg
     8941 cgggcgtctg atcatcgacg ggcgcgatga gaccgacaag cccgtcaaca agcgcgacat
     9001 cggtgttgta ttccagaatt atgcattgtt tccccacctc accgtgaggg aaaacgtctc
     9061 gtttccgttg cagatgcgga aaatagcggc agcggatctc cgggaaaagg ttgaagcgac
     9121 gctcgccatg gtcggattgt ccgatttcgg cagccgcttc ccgcgagaac tgtccggcgg
     9181 ccaacagcag cgcgtggcgc tcgcccggtg ccttgtctat cagccctcgc tgatcctgat
     9241 ggacgagtcc ctgtcggctc tcgaccgcaa actgcgggag agcatgcaga tcgaaatcaa
     9301 gcggctgcat cgcgagaccg gcgcaaccat catcttcgta acccacgacc aggaagaagc
     9361 gctcgcattg gcggaccgca tctgtctgat gaatgaaggg cggatcgaac aattaggcac
     9421 gcccgaggac atgtatgagc agccccgcag cgcgtttgtc gccgatttca tcggcatttc
     9481 gaatgtcatg cgcggcaggg tccagtcagg cggaatgatc cggaccgatg acggcagtct
     9541 gccgctcccc gacggctgcg aggcgcgggc cggcgaggac ggtgcgctga tcgtccgccc
     9601 ggagaagatg ttgctctgcg ctatcgatga agcgttcgtc tccgggcgcg tcgaggaaac
     9661 gatttacgct ggatccgaga ccaagctgct ggtgcggctg cctagcggca cactgatcac
     9721 cgtgcggcgg caggcaggtt tgcccggcgt ggcgatcggg gagcacgtct ctatccgatg
     9781 ggacaagcaa caggcacgcc ttttggagac cggattggtt tcgatgattg agtgaggaca
     9841 tgttccgcag gtccttcgcg aaggttgacc ttgcagagac gagcttcgtg cgcggagcag
     9901 cagaagcgaa cgcgcaaaga cggcgaaccc aggcgaatcg aggagaaaca ggatgtcgac
     9961 ttttgagacg atcacgcggc gcaagcttct gaaagccgcg agcgtcgctg cgaccggcgt
    10021 cgcgatgccg gtggtttgga ccaagagggc gcgcgctgcc gaagagatca cggttgccga
    10081 tgtgggcggc gccaccgctc cggcgctgcg ggcggggttt tacgacccct tcgaaaagga
    10141 gaccggaatc cgcgtcatca atgttgcgca cgaatccgat ccggtaaccc aattcaagct
    10201 ggtcgtcgat accggcagca agatatggga cgtctgcatg gtcacgccgg acgacgtcgc
    10261 gcgcctgacc gctgacaaga actatctcga atctctggat atcgcaccaa gcgaagacca
    10321 ggatctcatt ccgggagcac tcaagccgaa ctggctcggc ttttcggttt acgcgatcgt
    10381 catggcctat cgtagcgacg tttacaaaac cgaagcgccg aagagctggg ctgatttctg
    10441 gaacaccacc aagtttcccg gacgccgagg cttgtatcga agtccgaaag gcgtgctgga
    10501 ggccgccctg ctggccgatg gcgtggcgcc gaaggatctc taccccctgg acgtcgatcg
    10561 ggcttttgcc gtgctcgaca aggtgcggtc tcaggtcgcg gtgtggtgga ccagcggtgc
    10621 gcagaacacg cagatccttc agagcggcga aatcgacatg tcggatacgt ggtcggcgcg
    10681 cgctttcgcg gcgatcgatt caggcgctcc agtcaacatc gtctggaacg gcctttattc
    10741 gatcgacggc tggtcgattc cgcttgggac tccgaagctg aagcaggcgc gcgcgttcgt
    10801 gcggttctgc atgcatcccg aacggcaggg ggcatattcg agcatggttg cgaacggacc
    10861 cagcaatctg aaggcctata atttcattgc cccggcacgc gccaaggtgc tgccgacgta
    10921 ccctgaaaat ctcaaaggcc ttaccgagcg tgattttgcc ttttggggga aaaatttcgc
    10981 cgcagtctcc gaccgctttc aggaatggct gctgatgggc ggacatcgct gacgcctgct
    11041 tcggcagaga agacctgaga tggtgtgtcc ataagccgcg ccgcatttga gaaaattcca
    11101 aggggatgac gatgaacaaa aagaagcaaa tgacgatcgg cgcgttgatt catgccaacg
    11161 gatctcattc ggcgtcctgg ttattgcagg aagcccaccc tcatgcgtcg accgacatcg
    11221 attactatcg cggcatcgcg caattggcgg agcggggaaa gttcgatttc ttcttcatcg
    11281 ccgatacgcc ggccgcgcgt accgaaaatc ttcgggtctg gagccggtcg cccctgttca
    11341 tgaacgtgct ggagccgatc acgctcttgt ccgcggtcgc aggtgcgacc acccatatcg
    11401 gccttggcgc gacggcatcg accagctttt tcgagcctta taacctcgcc cggcagtttg
    11461 cctcgctgga tcacatctca catgggcggg cggcctggaa tgtggtgacg tcggccaatg
    11521 attacgccgc gcgcaacttc gggctcgatc gcctgccacc tcatggcgag cgttacgcca
    11581 aggcgcgcga attcgtcgaa gtcgtcgagg cgctttggga tacctgggag gacggcgcct
    11641 tcgtctatga tcgggatgcc tgtctcagct ttctgcccga gaaattccat gggctggagc
    11701 acaagggcaa gtatttcacg gtgcagggcg cgctcaatat cgagcgctcc ccgcagggcc
    11761 ggcccgtcat catccaggct ggcgcctccg acaccggccg ggatttcgcc gctgaaaccg
    11821 ccgaagtggt gttcgggtcc ggcgcctcgc tcgacgaggc aaaggctttc tatcgtgatc
    11881 tcaaggggcg gatgggaaaa ttcggccgcc agccggacga gttgaagatc gcgaccggca
    11941 tctcggtggt gatcggagag agcgagcagg aagcccgcga caagctccag agctggcaag
    12001 aactcatcca tcccgatgtc ggcgtgctgc gtctcggaca ggatctcgaa accgatctgt
    12061 ctgatttgcc gttggacgag ccgatcccgg agcaccggat tcctcagtcg tccaacttcc
    12121 acaaggcgta tttcgaccag attgtcggca tgatcagcca acggatgacg ttgcgcgaaa
    12181 tgtcgatgcg ctataatcgc agcaaggcca cgttctgcgg caccgcgctg caggtggccg
    12241 accatatgga agcctggatg gcggcggacg cctgcgacgg cttcatgatc tcttttctcg
    12301 cattgccaag cagcctcagg gaattcgtcg acacggtggt gccggagctt cagcgccgcg
    12361 ggctgtttcg taccgactat gccggccgca cgctacgcga tcatttagga ttgaagcatc
    12421 ccgagaaccg ctatgtgtcg caggcgcgtg ccgcgtcgat cgcgaaacgc gcctgagcca
    12481 tgccgctcga tcatctcccg ccaagatgcc aggtcgatga ccgatagcgt gtctctcgcg
    12541 accgttgcgc tcgcgcggca gcggaagctg aggatcgagc cgctttggct tgctcttccg
    12601 ggagtcgtgt tcctggttct gtttctgctg ctgccgacgg cgcagatgct gtcgaggagc
    12661 ctgttcgaca aggtcacggg agcgctaacc cttgcggctt ttgcgaggat ctggaacggc
    12721 ggaccgtatc ttgccgtcct gtcgacgacg ttctccgttg ccttttggac gacgctgctg
    12781 tgcctgacgc tcagttatcc cctggcctat tggttgtgct gcaagtcgcc gcgtcagcag
    12841 cgcatcgcca tgctgtttgt tttgctgcca ttctggacca gcgcattgat caagaatttt
    12901 tcctggctgg tcctgctcgg gcggaacggg atcatggcga aaatcatggc gtccgccggg
    12961 atgacgggcg gagaccagct gctgttcagc cgcaccaccg tcgttttcgc gatgacgcac
    13021 acgctgctgc cgctcgccgt cgtcaccatg cttccggtca tgaatcaaat cgatcggcgc
    13081 ctgcccatgg cggcggcgac gctgggcgcg agcggtgcgc aagccttctg gcaaatcttc
    13141 tatcagctct ccatgcgcgg cgtggcagcg gcggggctgt tggtctttgt cgcgtcattg
    13201 ggcttcttca ttacgccggc gctgctgggg ggaccgcggg agaccatgat cggccaattg
    13261 atcatcctgc agatcaacga gctgcagaac tggcaactcg gcagtgccct tgcggcgatt
    13321 ctgctcgtct cggcaatcgc gatgtgcatg gtttatgacc gcatcttcgg tttgtcgtcg
    13381 gtgtccgagg gcagcgagcg gcagacatct cccggcggtc tcgcgcggct tgcgggtctt
    13441 gccgcggcgc ggttcgccgg actggtgttc ggtgccatcg aggagttctg gaaacgcaat
    13501 attcgtggcc ttcgcggcag cgcaattctc tctatctacg cctgggtcgt catcgccgtc
    13561 ctgctggtgc cgatcattgc cttcgtcccg atggcgttca cccggtcgac cttcctttca
    13621 tttccgcctc aaggtttcag tctgcagtgg ttcgagcagt tcatggcgtc gccattgtgg
    13681 cttggcgcga tgatccgatc cttcggcatc ggtctcgcga cggcggcaat caccgtggtg
    13741 atcgcgtcgt tggccgcgct gggcttggcc cgcagccgca gccggctggc cggtgcggtg
    13801 ttcctgttgt tcttgacccc gatggtcgtc ccctcgatcg tcatcgcgat cgccctgttc
    13861 tacctgtttg cccaggtctc cctggtggcg accaacctcg ggattatcat cgggcacacg
    13921 gttatcgcca ccccggtcgt attcgtgatc ctgttggcca ccttcaaggg acacgactgg
    13981 cggctcgatg cggcggcgtc gacacttggg gctagtcgcg cccagacgtt ctggcgtgtg
    14041 acgttgccgc tcgtcaaagg cggatttgcc gttggttttg tcaccggctt tcttcaatcc
    14101 tttgaggaat tgacggttgc cttgtttgtc ggcggtggat tgaagacgac gctgcccaag
    14161 cagatgtggg acggaattct tcttcaggtt agtccggtta ttgctgctgc gtcggtcgtc
    14221 gtgctggtgg ttgtgatcct gatgttcgcg ataatggagt gtgttcagga gcgcagtgct
    14281 gcggcacgac gctattcctg attgccatgt tggtcgagca gaaataccgg cgtccgatcg
    14341 gagggacaga cccgcttcgg cggacggaag gctatgatct cctctcgtcg acaatgcctt
    14401 tgcaattcat tgtgcgacac gtcgccttcg cccaccggcg gcatctggtg caattcattc
    14461 gtgttgtccg gccgcaacta tcgcccccat tttcattcaa gtcggccatg agaatgcttt
    14521 caaaagaaac gacctcagcg cgggggtaaa gcggacgttt accgaggagc tgacgtccga
    14581 aagtgccgtg aggggacatc tcagcgttga gtgcaaccta ctcgatcacc tcaacggtgg
    14641 ccgggataac cctcatgcta ttgctgcgtc gcgacgcgcg cgctaggctt ggccgaattg
    14701 gtgcaggagg ccccccgtga tccagtccga ggacagtaag atttccgaag atcgccgaga
    14761 agcgctgcgc tacgcccttg ccgtcggcag cggcgcagcg cttgctgccg ccgtcgcaag
    14821 cccggcatcg gcacaagcaa ccccggacaa cacgctcgat cgcatccgcg ccaacaaagc
    14881 gctccgcatc gcggtactgc cgggagagct gccttacttc aacaaggacc ttgctaccgg
    14941 caactggtcc ggcttctcga tcgagatggc caacgatatc gcgaagcttc tcgacgtcaa
    15001 acttgaatat accgagtcga cttacggaaa ttcgatcctc gacctgcaat cgaacaagat
    15061 cgacctcggc tttgccctc
//
LOCUS       DASWGM010000514         9274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_590324,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000514 DASWGM010000000
VERSION     DASWGM010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590324"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGA15_14945"
     CDS             <1..300
                     /locus_tag="VGA15_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9589030.1"
                     /translation="KCLACHAIGDGAKNKVGPELNGLDGRKSGTAPDYNYSDANKNSG
                     ITWNEAQFKDYIKDPKAKIPGTKMAFAGIKNDKEVNDLWAFISQYDKDGKDGKTK"
     gene            complement(319..1311)
                     /locus_tag="VGA15_14950"
     CDS             complement(319..1311)
                     /locus_tag="VGA15_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9589031.1"
                     /translation="MRKICKVTLNDEPHLANCGDLVLDWALMNGIDLPHDCRAGVCGA
                     CRVRLVEGRVFGGHEQGDDMIHACQARIVSDIEIVTEAVPDQVTMTGRVTQMVRLAPD
                     VIGVDVELPRPLHHLPGQFCKAQFRGFPSRCYSPTYPLEGRADDRMLHFHIRRFPDGV
                     VSSELGRKIRIGHRMKVTGPFGSAFLRPNHSGSIVLVASGTGFAPMWSIAVAAILERP
                     QRELVFVVAARKLQSLYMHSALCRLALFPNVTIIPTVSEPQSISPAIRSGRPTDHLPS
                     LSPNDVVYTSGAPVMTDAVARIARAAGARCFTDPFVANPDQSSAPKPVAARMSR"
     gene            complement(1314..3599)
                     /locus_tag="VGA15_14955"
     CDS             complement(1314..3599)
                     /locus_tag="VGA15_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEX9589032.1"
                     /translation="MRKRKTDYGTIILHWLLVAAFGVAFVTGMRIATEAPERSWINSF
                     DAVLPQNVWVMHMQAAVVLLAVAFAYTIYVRRSGLGRRIRLDKVRLRGLVGRGQARRG
                     AVNILLYWVFFVTMLALMASGGLMYFGYFAGYDVATLHWYATWVVLGFSGLHVLSHLR
                     IGGTSQLLRIFRPTGLSAPPPQLDAIELLTMLAEQSARLQPESGGADKTASETRMQPV
                     QHETRVQPAQRTAPVRPLQRETRVQPVRREAPLQPVQRENRVQPVRREAPLQPVQRET
                     RVQPVRREALLQPVQRETRASPVQREALLQALLREAPLQPVQRDAPLQPVQRDALLQP
                     IQREAPLQPAQRPKDATRGRPPVRSAATHPAPGPAAKSRTSKPRNPTFQSNPFVVAAA
                     IAIAGASIVVATDRLAVDQLQIHRISSADAPILDGDTSDRAWRSIQPFSMMTNQGGNF
                     DGKGESRIDIRAVHDGTWAYFLFTWEDPTRSLKHLPLIKEIDGWHMLHDGYESGDEHD
                     YNEDKFSVLLTTLDATLAGDRTFHAGTHPIPEAPPTMTGRGLHYTPGNDVYVDVWLWK
                     ATSSGSTGWMDDSHFGPPLDPTPMQVRNVVPYRGGFAPDPGTANYADNFIMSDEPAVD
                     GKRMITPRRLPRDLAKTAAAMGEIDLDPNHGESDGARWFMTEAESVPYSSGQDVTVPV
                     GTVIPGVIIGGEFSGDRADIRCAARWASGHWALEVARRLDTHSQYDVPLQTGVFMRVA
                     AFDHSQIRHTRHVRPIRLEVE"
     gene            4583..6340
                     /locus_tag="VGA15_14960"
     CDS             4583..6340
                     /locus_tag="VGA15_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX9589033.1"
                     /translation="MTLRLRESARRMIGIAAFSGVINILMLSGSLYMLQVYDRVIPSR
                     NVSTLLGLSLMVLLAYVAQGYFDALRSRMLCRVATLFDAGLQEAIHVALATLPLRGVK
                     PMLMQQPLRDLDQVRAFMSSMGPTAFLDMPWIPIFLIGLFLFHPAIGITALLGTAAII
                     AMTLLTERMTRGAAKSAMDSSAQRQVLADATQRNAEVIRALGMMDRFTARWSRANERY
                     LRENIRATDVYANLGSGAKVLRYVLQSGMLGIGAYLVVADKASGGIMIASSIMMGRAL
                     APVEVALSTWKQLIAARLSIKRLREICKATAAPVVPPVVLPRPSRELSVQNLAVAAPA
                     GEKTIVSNVSFSLKAGMGLALLGASASGKTSLSKALVGIWPAQQGVVRLDGAALDQWR
                     NEDLGRYVGYLPQEVGLFDGTVAENICRFDEQATSDAILRAAQIAGVHEIILRLPDGY
                     ATRIGEGGMALSAGQRQRVGLARAIFGDPFLVVLDEPNANLDAEGEAALTRAIQILRH
                     NKCIVIVISHRPSALAALNMAMILYEGKAIAFGPSEEIFARVRTAQGLPPPAQARQPV
                     VAPNAKTVRRAPLAESVPS"
     gene            6358..8499
                     /locus_tag="VGA15_14965"
     CDS             6358..8499
                     /locus_tag="VGA15_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX9589034.1"
                     /translation="MRPGERAQHDDASAQRPAGEPRRDGVGPQGAALILELQRLRQAF
                     DPETSDDLPTPQARRPARDGSRPPSPGRQRTRPPKRKKSKMERAIAVCLEQLGFSAAP
                     SAGRINSAPAAKRVSSAPAAGRINTASPTGRINTASQAGRINSAPPSGRKGERLVEPN
                     LKPPPRRRSFVTLDNAIPMDLLKPAPELRGSDNKRASTPINQPRLAIPQSAPMDKPQQ
                     MDKPQLALPRPAGNVESKTTEPKTTLGQLIEKSRVGVSTGVAFLVNRDGLAHNDNAPG
                     DTLTLRTRRSYERELRVGLRVLLAGLVLGGGWMTLMPLAGAVVVPGNLVVQSNVKTIQ
                     HPTGGVVAEIPVHNGMRVAVGDLLLRLDPTQAQASLQVVSKQLDESRARIARLIAERD
                     GLPQPEVPAELAGRLDDGAVKDLLASEETQFKARASARYSQKDLLQSRVAQLGEQIGG
                     LDAQVASKAKQLELIAGELTGVQDLYDKRLVPITRLTTLQREAARIEGERGQLISAIA
                     ETKAKVGEAQLQIVRIDQDFRTEVVKELGEVQGKEGELVQRGVAARDMLDRIELRAPT
                     SGIVHQLAAHTVGGVIKAGDAIMEVVPDSDDLQVEARLQPNDIDQVRVGQKALIRFSA
                     FNQRTTPQIVGLVSFVSADVSHDQTASAANVSFFTVRMTLPEDELRRLAGLQLVSGMP
                     AEVFMQTGSRTMMSYLMKPITEQLQRAFVER"
     gene            complement(8609..9247)
                     /locus_tag="VGA15_14970"
     CDS             complement(8609..9247)
                     /locus_tag="VGA15_14970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9589035.1"
                     /translation="MARAHIGSMTHLASRIIEHYERHATAWDCDRQNSAWNDKVWHDL
                     FIDRLTAGAKVLDLGCGSGRPVARHMAERGLLVTGVDSSPTMISLCRSRLPDHAWIVA
                     DMRELSLGRRFDGILAWDSFFHLDHDDQRRMFAVFAAHASGGAWLMFNAGPRHGEAIG
                     NYRGDPLYHASLSRGEYETLINRCGFEVIEHAADDERAGGRTAWLCRSREGA"
BASE COUNT         1773 a         2873 c         2904 g         1724 t
ORIGIN      
        1 aaatgcctgg cctgtcacgc gatcggcgac ggcgccaaga acaaggtcgg gcctgagctc
       61 aacgggctgg acggccgcaa gtccggcacc gcgccggatt ataactactc ggacgccaac
      121 aagaattccg gcatcacctg gaacgaggcc caattcaagg actacatcaa ggacccgaag
      181 gcgaaaatcc ccgggaccaa gatggcattc gccggcatca agaacgacaa agaggtcaac
      241 gatctctggg cgttcatctc gcagtacgac aaggacggaa aggacggaaa gaccaagtaa
      301 cgtcgcggcg ccgcggcgtc atcgcgacat ccttgcggca accggctttg gcgcgcttga
      361 ctggtccgga ttcgccacga aggggtcggt aaaacacctg gcgccggcgg ccctggcgat
      421 ccgtgccacg gcatcggtca tgaccggcgc gcctgaggtg tagacaacat cgttcggcga
      481 cagacttggc aaatggtcgg ttggccgtcc gctgcgaatg gcgggggaga tgctctgcgg
      541 ttccgatacg gtcggtatga tcgtgacatt gggaaacagc gccagacggc acagcgcgct
      601 gtgcatgtac aacgactgca gctttcgcgc cgcgaccacg aataccagtt cccgctgcgg
      661 ccgctccagg atcgctgcaa ccgcgatcga ccacatcggc gcgaaaccgg tgccgctggc
      721 gaccagaaca atgctgccgg aatgatttgg cctgagaaag gcgctgccga acggacccgt
      781 caccttcatg cggtgcccga tgcggatttt tcgcccgagc tccgaggata cgactccgtc
      841 agggaatctg cggatgtgaa aatgcaacat gcgatcgtcg gcgcgaccct cgagcggata
      901 ggtcgggctg taacatcgcg acggaaaccc ccgaaattgc gccttgcaga actgaccggg
      961 aaggtgatga agcggtctcg gcagctcgac gtccacgccg atcacgtcgg gcgccaggcg
     1021 aaccatctgg gtcacgcggc ccgtcatcgt gacctgatcg gggacggcct cggtcacgat
     1081 ctcgatatcg gagacgattc gcgcctggca ggcgtgaatc atgtcgtcac cctgctcgtg
     1141 gccgccaaac accctgccct caacgaggcg cacacggcag gcaccgcaaa ccccggcgcg
     1201 acaatcgtgc ggcagatcaa tgccattcat caaggcccag tcgagcacga ggtcgccgca
     1261 attcgccaag tgcggctcat cgttcagggt taccttgcaa atcttccgca ttattattct
     1321 acctcaagac ggattggccg gacgtgtcgg gtatgcctga tctgactgtg atcaaaggcc
     1381 gcgacccgca tgaagacccc ggtctgaagc ggaacatcgt attggctgtg ggtgtcgagg
     1441 cggcgcgcca cctccagcgc ccagtgcccg gaggcccagc gggccgcaca tcggatgtcg
     1501 gcgcgatcac ccgagaattc gccgccgatg atgactcctg gaataaccgt ccccaccggg
     1561 acggtgacat cctgtcccga cgaatacggt accgattccg cctccgtcat gaaccagcgc
     1621 gcgccgtcgc tctcgccatg attggggtcg aggtcgatct cccccatcgc cgcggctgtc
     1681 ttcgcaagat ccctgggcag ccggcgcggc gtgatcatcc gtttgccgtc gacggcgggc
     1741 tcgtcgctca tgataaaatt gtcggcatag ttcgcggtgc ccggatctgg cgcgaagccg
     1801 cctcgatacg ggacgacatt gcgcacctgc atcggcgttg gatccagcgg tggaccgaaa
     1861 tgcgagtcat ccatccagcc tgttgaaccg ctacttgtgg ccttccaaag ccagacgtcg
     1921 acataaacgt cgtttccggg cgtgtaatgc aggccgcggc cggtcatggt aggcggcgct
     1981 tcgggaatcg gatgggtacc ggcgtggaac gttcggtcgc ccgcgagcgt tgcatccaaa
     2041 gtggtcagca ggaccgagaa tttgtcctca ttgtagtcgt gctcgtctcc gctctcatag
     2101 ccatcgtgaa gcatgtgcca tccgtcgatc tccttaatga gcggcagatg cttgagcgaa
     2161 cgtgtcggat cctcccatgt gaacaggaaa tacgcccaag tgccgtcgtg caccgcgcga
     2221 atatcgattc tcgattcccc cttgccgtca aaattccctc cctggttggt catcatggag
     2281 aatggctgga tgcttcgcca ggctcgatcg gatgtgtcgc cgtcgaggat gggcgcatcg
     2341 gcgctgctga tacggtgaat ctgcagttgg tcgacggcca accgatcggt tgcgacgacg
     2401 atcgaagcgc cggcgatcgc gatcgccgcg gcaacgacaa aaggattgga ttggaacgtc
     2461 gggtttcgcg gtttcgacgt tctgcttttt gccgctggtc ccggcgccgg atgcgttgcc
     2521 gcactccgta caggcggccg accgcgagtg gcatccttgg gccgttgtgc tggctgcaga
     2581 ggtgcctctc gctggatcgg ttgcaggagt gcatctcgct ggaccggctg caggggtgca
     2641 tctcgctgga ccggctgcag gggtgcctct cgcaggagcg cctgcaggag tgcctctcgc
     2701 tggaccggcg acgcccgcgt ctcgcgctgg actggctgaa ggagcgcctc tcgccggacc
     2761 ggctgcaccc gtgtctcgcg ctggaccggt tgcaagggcg cctctcgccg gaccggctgc
     2821 acacggtttt cgcgttggac tggctgcagc ggcgcctctc gccgcaccgg ctgcacccgc
     2881 gtctcgcgct ggagtggccg cacgggcgcc gttcgctggg ctggctgcac ccgtgtctcg
     2941 tgctggactg gctgcatccg ggtctccgac gctgtcttgt cggcgcctcc ggattccggc
     3001 tgcagccgtg ccgattgctc ggccagcatc gtcagcaatt caatggcatc gagctgcggt
     3061 ggcggcgctg acaggcctgt aggccgaaag attcgaagca actgcgatgt tccgccgatc
     3121 cgaaggtgcg acaggacatg aaggccggag aagccgagta ccacccaggt cgcgtaccag
     3181 tgcagcgtcg ccacatcgta gccagcgaaa tagccaaaat acatcaaccc gccgctggcc
     3241 atcagcgcca gcatcgtgac gaagaacacc cagtacagca ggatgttgac ggcgcctcgc
     3301 ctcgcctgcc cgcgaccaac aagtccccgc aggcgaacct tgtccaaccg gatgcggcgc
     3361 ccgagaccgg accgacgcac atagatcgtg tatgcgaacg ccaccgccag cagcacgacg
     3421 gccgcctgca tatgcatcac ccacacattc tggggtagaa ccgcatcgaa cgaattgatc
     3481 cagcttcgtt ccggcgcttc ggtcgcgata cgcattccgg tgacgaaggc aactccgaac
     3541 gcggcgacaa gcagccagtg cagaatgatc gttccgtagt cggttttgcg ttttctcatg
     3601 cgcgcgaccc gctacccgag aggcggcaga ttgcggcatc catccgatac gcgagtctca
     3661 cgcggatttc acgcgtagca tgccacaacg tgttgtatcg cattgcacgg ccattgattt
     3721 cgcgccgtgt cgtgtttcgc ctagagtcgt tgttactgtc agacaaatca cccgccccaa
     3781 atcacgtttc gccgaaatcg gcgaacgaag aatccttctc tttatgcggg cgcgcgcccg
     3841 caacgtgtcg atggttcaaa cagcaagacc cgggctgaat tgaactatcc ggctaacctc
     3901 cggcacctat ccccatagat gatgcgtggg acccggaggt catgctggtc ggagcgctat
     3961 ccccccatgt gacggtgttg tcgggtcgga cgaaggaacg cgatccatcg ggggcgcgga
     4021 cattgatcgg taccccgacc atcgcgcgca tgatgtcgcg cgcatgatgt ccgcgtgtgc
     4081 gatcctccgt gtgtgcgatc cattgtattc tttcccttac ttaccagtgt ttcatgactg
     4141 atgagcgcaa ccgttcgacc gcgcagcggc gtgaagcgca agatgaaaga tcatttacct
     4201 tcgtggcgtc tttcagccga tccgggggcc aatgcgcgca gaagaaaggt tataggcgga
     4261 cgaatctatg acgatgggag gttcttgagc accgaccatc gcggtcaacc ctttcgcaaa
     4321 atgcgcgacg ccaacagcag cgtctctaca cttgtggaca cttgtggcga ttctttgtgc
     4381 cagcgaaatg tcgacgggga ttggcgacgg tctcattctt gccggaaaat ggtctgtatc
     4441 gcctgtcaag gagaggtgat cgccgatcga tatcggcctg atggagagcc gcattggcat
     4501 gagtgtcgct cagaatcacg cacagacatt cctttccggc ggagtatcgc ccgtgccgcc
     4561 caccgctgcg accgatgatc cggtgacgct caggctgcgc gaaagtgcgc ggcgtatgat
     4621 cggcatcgcc gcattcagcg gggtgatcaa tattctgatg ctgtcgggct cactctatat
     4681 gttgcaggtt tatgatcggg ttattccgag ccgcaacgtc tcgaccttgc tcggcctctc
     4741 gctgatggtg ctgttggcgt acgtcgcgca aggatacttc gacgcgctgc gctcgcgaat
     4801 gctctgtcgt gtagcgacac ttttcgatgc cggcttgcag gaagcaattc atgtagcgct
     4861 cgccactctg ccgctgcgtg gcgtgaaacc gatgctgatg cagcagccct tgcgtgatct
     4921 ggaccaggtg cgcgcgttca tgtcgagcat gggaccgacg gcgttccttg acatgccatg
     4981 gatcccgatt tttctcattg ggttgtttct gtttcatccg gccatcggta tcacggcgct
     5041 gcttgggacc gccgcgatca tcgccatgac attgctgacc gagcgcatga cgcgcggcgc
     5101 ggccaagtcc gcgatggatt ccagcgctca gcgacaggtg ctggcggacg cgacgcaacg
     5161 caatgccgag gtcatccggg cgttgggcat gatggatcga ttcacggcgc gctggtcgcg
     5221 ggccaatgag cgctatttgc gagaaaatat ccgtgccacc gacgtgtatg ccaatctcgg
     5281 atcgggtgcg aaggtgctgc gctatgtgtt gcaatcgggg atgttgggca tcggtgcgta
     5341 tctcgtcgtg gccgacaagg cgtcgggcgg catcatgatc gcgtcgtcga tcatgatggg
     5401 ccgcgccctg gcgcccgtcg aagtagcact tagcacctgg aaacagctta tcgccgcgcg
     5461 tctgagcatc aagcgtttgc gcgaaatctg caaggcgacc gcggcgcctg ttgtgccgcc
     5521 ggtggtgctg ccccgtccca gccgcgagct ttcggtgcag aatctcgccg tcgcggcgcc
     5581 ggctggggaa aaaaccatcg tttcgaatgt ttcgttttcg ctcaaggccg gcatgggact
     5641 ggccctgctt ggcgcgagcg cgtcgggcaa gacctcgctc tcgaaggcgc tggtcggaat
     5701 ctggccggcc cagcaaggcg tcgtgcggct cgatggagcc gcgctcgatc aatggcgaaa
     5761 cgaagacctc ggccgctatg tcggctacct gcctcaggag gtcggtctgt tcgacggcac
     5821 cgttgccgaa aacatttgcc gtttcgacga gcaggcaaca tccgatgcca tcctgagggc
     5881 cgcgcagatc gcgggcgtgc atgagatcat cctgcgtctg ccggatggct acgcgacgcg
     5941 aatcggagaa ggcggcatgg cgttgtccgc ggggcagcgg cagagagttg ggctggcgcg
     6001 ggcgatcttc ggcgatccgt tcctcgtcgt gctcgatgaa cccaatgcca atctggatgc
     6061 cgagggagaa gctgccctga cccgtgctat tcaaatcctg cgtcacaaca aatgcatcgt
     6121 gatcgtcata tcgcatcggc cgagcgctct cgccgcattg aatatggcga tgatcctcta
     6181 cgaaggtaag gcaatcgcgt tcggcccaag cgaggagata ttcgcacgcg tgcgtacggc
     6241 gcagggactg ccgccgcccg cacaggcaag acaacccgtg gtggcaccca acgcgaaaac
     6301 ggttcgacgc gcgccgcttg ccgagagcgt cccgtcatga ggcccgaagg ttccgtgatg
     6361 agacctggag aacgcgcgca acatgacgac gcgtccgcgc aacgccctgc cggcgagccg
     6421 cgccgcgacg gcgtcgggcc gcaaggcgcc gcgctgatcc ttgaattgca gcgcctgcgg
     6481 caggcgttcg accccgaaac ctcggatgac ctgcccacac cgcaggcgcg ccgaccggct
     6541 cgcgacggtt cacgtccgcc ttcgcccggg cgccagcgga cacgcccgcc gaagcgaaag
     6601 aaaagcaaga tggagcgggc catcgcggtc tgtctcgagc aattgggatt cagcgcggcc
     6661 ccttcggctg ggcgcatcaa ttccgctccc gcggccaaac gtgtcagttc cgctcccgct
     6721 gctggacgta tcaataccgc ttcgccgacc gggcgcatca ataccgcttc ccaggccggg
     6781 cgcatcaata gcgctccccc gtccgggcgc aaaggcgaac gtctggtcga accgaatctc
     6841 aagccgccac cgcgccgtcg cagtttcgtc acgctcgaca acgccatccc gatggatctg
     6901 ttgaagcccg cacccgagct tcgtggatcg gacaacaaac gggcgtcgac gccgatcaat
     6961 cagccccgat tggccatccc gcagtcagcg ccgatggata agcctcagca gatggataag
     7021 cctcaattgg ctcttccgcg tccggccggc aatgtcgaat ccaaaacgac tgaacccaaa
     7081 acgactcttg ggcagttgat cgagaaaagc cgcgttggcg ttagcacagg cgttgccttc
     7141 ctggtcaacc gcgacggctt ggcccacaac gataatgcgc cgggtgatac cctgactctg
     7201 cggacccgac gctcgtacga gcgcgagttg agggtcggcc ttcgggtgct gcttgccggc
     7261 ctcgttctgg gcggtggctg gatgacgctg atgccgctgg caggtgcggt cgtggttccc
     7321 ggaaatctgg tggttcagtc caacgtcaag accatccagc atccgaccgg cggggttgtg
     7381 gcggagattc cggtccacaa tggcatgcgc gtcgccgtcg gcgatctgct gttgcggctc
     7441 gacccgaccc aggcgcaagc cagtcttcag gtggtgagca agcaactcga tgaatcccgg
     7501 gcgcggatcg cccgactgat tgccgagcgc gacggcctgc cgcagccgga agttccggcc
     7561 gaactggcgg ggcggttgga cgatggcgct gtcaaagatc tgctggcgtc cgaggagacg
     7621 cagttcaagg ctcgcgcgag cgcgcgttac agccagaagg atcttctgca aagccgcgtc
     7681 gcgcaactcg gcgaacagat cggaggcctc gatgcccagg tcgcgtccaa ggcgaaacag
     7741 ctggaactga tcgcagggga acttacggga gtccaggatc tgtatgacaa gcggctggtg
     7801 ccgatcacgc ggctgacgac attgcagcgc gaggcggcgc ggatcgaagg cgaacgcggc
     7861 caattgatct ccgcgatcgc cgagacaaag gctaaagttg gcgaagccca gcttcagatc
     7921 gtacggatcg atcaggattt tcgtaccgaa gtcgtcaagg agctcggcga agtgcaaggc
     7981 aaggaaggcg aactcgttca gcgcggcgtc gctgcccggg atatgctcga tcggatcgaa
     8041 ctccgcgcgc cgacttcggg gattgtccat cagctcgcag cgcatacggt tggcggcgtc
     8101 atcaaagccg gcgacgccat catggaggtg gtgccggatt cggacgacct gcaggtcgag
     8161 gcgcggctgc aacccaacga tatcgaccag gtgcgggtgg gacagaaggc gttgatccgc
     8221 ttctcggcct tcaaccaacg cacgacaccg cagatcgtcg gcttggtgtc ttttgtgtcg
     8281 gccgatgtca gccatgatca aacggccagt gcggccaatg tgtcattctt cacggtcaga
     8341 atgacgctgc cggaggacga acttcgccgg ttggccggac tgcagcttgt ctcgggcatg
     8401 cccgcggaag tcttcatgca gaccggcagc cggaccatga tgagctacct catgaaaccg
     8461 attaccgaac agttgcaacg cgcattcgtc gagcgatgag ccgttgcgtc gagtgcgagg
     8521 aaacccagga tcgggcggag cgagtgaagc cgcgatcgaa gcaggtcccg ctgagctgaa
     8581 ggtccgaatc gggacacggt gccacaactc acgcgccttc ccgcgatctg cagagccacg
     8641 ccgtgcgccc gccggcgcgc tcatcatcag cggcgtgttc gatcacctcg aacccgcaac
     8701 ggttgatcag ggtctcatac tcgcctcgtg agagactggc gtgatacaag gggtcacccc
     8761 gatagttgcc gatcgcctct ccgtgccgcg gtcctgcgtt gaacatcaac caggcaccgc
     8821 cggatgcatg cgccgcgaac accgcgaaca tgcggcgctg gtcatcatgg tcgaggtgaa
     8881 agaaactgtc ccaagccagg atgccgtcga agcgccgacc tagcgacaac tcccgcatgt
     8941 cggccacgat ccacgcgtga tcgggtagac ggctgcggca aagcgatatc atcgtcggcg
     9001 acgagtcaac gccggtcacg agcaagcctc gctcggccat atgtcgtgca acaggccgtc
     9061 ccgagccgca gccgagatcc agaacctttg ctcctgccgt cagtctatcg ataaagaggt
     9121 catgccacac tttatcgttc caggcgctgt tctgacggtc gcagtcccac gccgtggcgt
     9181 gtcgctcgta gtgctctatg atgcggcttg cgaggtgcgt catgctgccg atatgggcgc
     9241 gcgccacggt cgatcaagac gggcactacg cgtt
//
LOCUS       DASWGM010000515         5955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1724453,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000515 DASWGM010000000
VERSION     DASWGM010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724453"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1386)
                     /locus_tag="VGA15_14975"
     CDS             complement(193..1386)
                     /locus_tag="VGA15_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9589036.1"
                     /translation="MTRPFEGVKILDFTQVLAGPYASYQLALLGADVIKVERREGEDM
                     RRTPLSREWADRGLAPGWQAINGNKRSLTLDLQKPEAITIVKQLAASADVVMENFRPG
                     VMDKLGIGYAALSAVNPSLIYCAISGFGQTGPERSGAGYDGKIQAMSGIMAITGHEET
                     GPTRAGFAVCDVLSGATAAFGVSSALFQRTHTGKGQMVDVSMLEATLAFLSGQVADYT
                     VAGHRQQLSGNQAVSRRPTANLFKAGEGYLLLAVNSEKQYRSLMGALGRADTLEDPRF
                     ADWFARRENEPALRAIIEEALLKKDPREWEKILEAAGAPCASIWKVEEVIDHPQISAR
                     QAVQEIDTPYGRLRFAGSGFQLAHGGGRLDSMAPELSAHTDEVLNSLGYDADVIADLR
                     ARNIV"
     gene            complement(1453..2382)
                     /locus_tag="VGA15_14980"
     CDS             complement(1453..2382)
                     /locus_tag="VGA15_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HEX9589037.1"
                     /translation="MPGSLDDQLGFAPDYDSPVPYMQRTRDYYTAIGYTTPYRWAHYV
                     DAPFQSLKKPLSQSRVTIITTAAPYDPAKGDQGPGAAYNGGAKFYKVYDGDTSKQHDL
                     RISHIGYDRKHTTATDSGTWFPLPLLLKAAASGRIGEVAPRFFGAPTNRSHRVTIDVD
                     APEILARCLADKVDVVVLVPNCPVCHQTSSLVARHLEAGGISTVVMGCAKDIVEHAAV
                     PRFLFNDFPLGNSAGKPHDIESQALTLELALGLLEHASGARTTMQSPLRWSEDASWKL
                     DYNNISQMSPEELARRRSEFDKQKEIARGNRAA"
     gene            2799..3068
                     /locus_tag="VGA15_14985"
     CDS             2799..3068
                     /locus_tag="VGA15_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEX9589038.1"
                     /translation="MSDISIPGGRIRSFVERVEHLDTELQELNESKKEVFSEAKAEGF
                     DVKILKEIIKLRKQDKDERDERDTLLDMYMRAMDTAGPQLAAKAA"
     gene            complement(3414..3719)
                     /locus_tag="VGA15_14990"
     CDS             complement(3414..3719)
                     /locus_tag="VGA15_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589039.1"
                     /translation="MKSDAAVFEHGGVIRMSEADILSRNIHALKELQRVAWRNLADPA
                     LTTFERREIRNEIKQSDAELRSYLEMISDRSRFRERPVESGVADGFGKFEFRLLAGN"
     gene            complement(3910..4458)
                     /locus_tag="VGA15_14995"
     CDS             complement(3910..4458)
                     /locus_tag="VGA15_14995"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HEX9589040.1"
                     /translation="MRNGFFFHRHRRALPAICATAWLCSGAPWVVPAGADPAPIKIAV
                     FDFELDDASAGAGIAGDPAADLAQLDRVTSEARRVISNSGRYRLVDVASVAGEGVKSR
                     SLRQCNGCEAAIALKLGADQSFVGVVTRISRTEYVVRFQIRDARTGAPIIARQSDLRI
                     GADYSWNRGAVSLINSSLLDKR"
     gene            complement(4494..5018)
                     /locus_tag="VGA15_15000"
     CDS             complement(4494..5018)
                     /locus_tag="VGA15_15000"
                     /inference="COORDINATES: protein motif:HMM:NF023113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2380 domain-containing protein"
                     /protein_id="HEX9589041.1"
                     /translation="MRHLLVAVLAIFLWPAPFCGKAQDGGNKTQRAAVAVIDFDYVDT
                     SGEARDQRGEHEARLAVFMRALKDDLARGDSFRPVTPTCAPNPCSLAGSTVRGLMAAA
                     REAGADILLIGGVHKMSTLVQWAKIEAIDPKTERVLFDRLFTFRGDTDDAWRRAEVFI
                     VGEITAVSRLPASR"
     gene            5213..>5955
                     /locus_tag="VGA15_15005"
     CDS             5213..>5955
                     /locus_tag="VGA15_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX9589042.1"
                     /translation="MPALKVDLKLRLSLRVAALAALCFAAAAAYALFETDRSTRERAD
                     SIAELVARDLALQQNQSSWVRGAPIQFPDLQRVAAALMAPGLCIAYRAPSGEIVQRLC
                     GGVAPDDTSAPRLFADLYQRVFDAGRESARPVGYGKETHGEAVASIDRQSLIAQSWRE
                     TSRLAAIMAGALVGLCVLVYATLADALRPTRAIRAGLERLAAGDLSARLPPFDLAELS
                     AVGNVFNHLAGSLQTTLSERNALTRRLIVV"
BASE COUNT         1074 a         1860 c         1855 g         1166 t
ORIGIN      
        1 gcggtctaca aaggacgtta gatcacgatc cgattagcac gatccgattg gattgaatcg
       61 gatcgtgatc tcatctcttt gtttgagcat gatctccggg caaacgcttc gcgtttgtcc
      121 cgagggaaaa ccggttccca cttttccgga tcatgctcta gcccgaggtt ccggcgtgac
      181 ggccgccgcc gctcagacga tgttacgcgc gcgcaggtcc gcgattacat ctgcgtcata
      241 gccgagcgag ttcagcacct catccgtgtg ggcgctgagt tcgggcgcca tggaatcgag
      301 cctaccgccg ccatgggcga gctggaagcc gctaccggcg aagcgcaagc ggccgtaagg
      361 cgtgtcgatc tcctgaacgg cttggcgcgc tgatatctgc gggtgatcga tcacctcttc
      421 gaccttccag atgctggcgc agggcgcgcc tgccgcctcg aggatcttct cccactcgcg
      481 cggatccttc ttcaaaagcg cttcctcgat gatggctcgc agcgccggct cattttcccg
      541 ccgcgcgaac caatcagcga agcgcgggtc ttccagcgtg tcggcgcggc cgagtgcgcc
      601 catcagcgac cggtattgct tctcgctgtt cacggcgagc aacaaatagc cttcgcccgc
      661 cttgaacagg ttggcggtcg gcctgcggct cactgcctga ttgccggata gttgctggcg
      721 atggccggct acggtgtagt ccgccacttg gcccgaaaga aacgccagcg tcgcctcgag
      781 catggagacg tcgaccatct gcccctttcc ggtgtgagtg cgctggaaca gcgcacttga
      841 cacgccgaac gcggcggtgg cgccggacag cacgtcgcaa accgcgaagc cggcgcgcgt
      901 tggcccggtc tcctcgtgac ccgtgatcgc cataatgccc gacatcgcct ggatcttgcc
      961 gtcgtagcct gcgcctgatc gttccgggcc ggtctggccg aagcctgaga tcgcacaata
     1021 gatgaggctc gggttgaccg ccgagagcgc ggcgtagcct atgccgagct tgtccatcac
     1081 accgggacga aaattttcca tcaccacgtc cgcgcttgcg gcgagctgct tgacgatggt
     1141 gatcgcctcg ggcttctgca ggtcgagcgt caggctgcgt ttgttgccgt taatcgcctg
     1201 ccaacccgga gcgaggccgc gatcagccca ttcgcggctg aggggggtgc ggcgcatgtc
     1261 ctcgccttcg cgccgctcca ccttgatgac gtcggcgccg agcagcgcga gctggtagct
     1321 tgcgtagggg cctgccagca cctgcgtgaa gtcaagaatc ttcacgcctt cgaacggtcg
     1381 ggtcacgtcg acgtcctcct gatggatatt ctttttgttc ttgcttcttg tacttgatgg
     1441 cgcggccggc ggtcaggcgg ctcgattgcc gcgcgcgatc tctttttgct tgtcgaattc
     1501 gcttctgcgc cgcgccagtt cttccgggct catctgagag atgttgttgt agtcgagctt
     1561 ccaggaggca tcctcactcc agcgcagcgg cgactgcatc gtggtgcgcg cgccggaggc
     1621 atgctccagc aggcccagcg ccagttcaag tgtcagcgcc tgcgactcga tatcatgcgg
     1681 cttgccggcg gaattaccca acgggaagtc gttgaacaga aagcgcggca cagccgcgtg
     1741 ctcgacgatg tccttggcac accccatcac cacggtcgaa atgccgcctg cttcgaggtg
     1801 gcgggcaacc aggctggaag tctggtggca aaccgggcag ttcggtacca gcacgacgac
     1861 atcgaccttg tcagcgaggc atcgggcaag gatttccggg gcatcgacgt caatggttac
     1921 ccggtggctg cgattggtcg gcgcgccgaa gaagcgcggc gcgacctcgc cgatccgccc
     1981 cgaggctgca gccttcagga gcaagggtaa gggaaaccag gtgccgctgt cggtcgcggt
     2041 ggtgtgcttg cggtcgtagc cgatatgcga gatgcgcaaa tcgtgctgct tggacgtgtc
     2101 gccgtcatag accttgtaga atttggcacc accgttatac gcagcaccag ggccctgatc
     2161 gcccttcgcc ggatcataag gggcggcggt tgtgatgatc gtcacgcgcg attgcgacag
     2221 gggctttttc agcgactgga atggcgcatc gacatagtgc gcccagcgat aaggcgtcgt
     2281 gtagccgatc gcggtgtagt agtcgcgggt ccgctgcatg tagggaaccg gggaatcata
     2341 gtcaggcgca aagccaagtt ggtcatcgag cgagcctggc atggttgcgt ccttcctgcg
     2401 ttttccagcg gagatcataa atcgaccttc ggcccgacta aggatagtcc gcttattcct
     2461 caaaaaacag acatcaaacc gcaaatgttc agcgtccgcc tatccttcca atacgcgaca
     2521 ttgaagcgca atatcgaagg cttcgcttga actgaaaggg ggccgcccac tgaggcgggg
     2581 tcggagaggc atgcttttcg atcgaccagc aatttagtgc aggcaattcc gtgggcggcc
     2641 cacacacgcg atagcgcacc gtgatccgat cgcgcgagca gcgtttgata ggctcccgct
     2701 ttcgatcgtc tgcaataagg cagccccgcg gatttgcgtt aacgcgttgg tcatgtcttt
     2761 gcgttcattt tcacgatctc atttaacaga gaggcaccat gtctgacatc agcattcccg
     2821 gcggacggat ccgttctttc gtagagcggg tcgagcattt ggacaccgaa ttgcaggagt
     2881 tgaacgagtc gaaaaaggaa gtcttttcag aggccaaggc tgagggtttc gatgtgaaaa
     2941 tcctcaagga aatcatcaag ctgaggaagc aggacaaaga cgagcgcgac gagcgcgaca
     3001 ctctgcttga tatgtatatg agggcgatgg atacagccgg cccgcaacta gccgccaagg
     3061 ccgcttgact caatcgaaat ccaggcttga cccaagtggt tggcccaagg gcttcgcgcc
     3121 ggagcggtga tcgggacaac ccggtcaccc ctcctgccgc gcgcgcccgg atcgcctgag
     3181 ccaaacagcc gagagcccgc gcgctgcgaa agagacccca ccctgggtca ggggttgagt
     3241 ttgccgggat ggggctaagt caagagtcgg tgccgacgac ttggcgtcgg cgaatgcaaa
     3301 atgcgatcgc gattcgggca agttaagggc gatgcagctc ggcacgccag ccgattggca
     3361 cgaccccgcg ctttcgatgc ggtgacgtct tgaattccag atgtcaggta ccgctagttt
     3421 ccagccagaa gcctgaattc aaacttgccg aagccgtcgg ccacaccgga ttccactggc
     3481 cgttctcgga agcgggaacg atcagatatc atctccagat aggaccgcaa ctctgcatcg
     3541 ctctgcttta tttcatttcg tatttcacgg cgctcgaaag tggtgagagc tggatcggca
     3601 agattgcgcc atgcgaccct ctgcagctct ttcagcgcgt gtatgttgcg acttaaaatg
     3661 tcagcctcgc tcatgcgaat caccccgcca tgttcaaaga cagcggcgtc agatttcacg
     3721 tcgatcccta ataaactctt aatggctgct cccgccgtgc gttccaggcg ggcgggcctc
     3781 gcctcccggc cattgtcggc gtgaacgggc cttggctgga aggcccggaa cctgatctac
     3841 cttgcctcga tcgccgccgg ttgatcccgg gtacgctgat gagcgtcgag cctggtctcc
     3901 cggaggcttt cagcgcttgt cgagcaagct gctgttgatc agcgagaccg cgccgcgatt
     3961 ccaggaataa tccgcgccga tgcgaaggtc gctttgccgt gcgatgattg gggcgccggt
     4021 gcgggcgtcg cggatctgga accgcaccac atattcggtc cggctgatcc gcgtcacgac
     4081 gccgacaaag gattgatcgg cgccaagttt taacgcgatg gcggcttcgc agccgttgca
     4141 ttgccgcaat gagcgcgatt tcaccccctc ccctgcgacg ctcgcgacgt cgaccagacg
     4201 atagcgtccg gagttcgaga taacccggcg cgcctcgctc gtgacccggt ccagttgcgc
     4261 gaggtcggcg gcaggatcgc ccgcgatccc ggcaccggcg ctggcatcgt cgagctcgaa
     4321 atcaaacacc gcgattttga tcggcgccgg atcggcaccg gccgggacca cccatggcgc
     4381 gccgctgcat aaccaggcgg tggcgcagat ggcgggcaac gcgcggcggt ggcggtgaaa
     4441 gaaaaatccg tttctcatgg gtggctccat ttctgccatt cgctgaaaag tcgtcagcgg
     4501 gaggccggca ggcgcgaaac cgccgtgatc tcgccgacga taaaaacttc ggcgcggcgc
     4561 caggcgtcat cggtgtcgcc gcgaaaggtg aacagcctgt cgaacaggac gcgctccgtc
     4621 tttgggtcga tcgcctcgat cttcgcccat tgcaccagcg tgctcatctt gtggacgccg
     4681 ccgatcagca ggatgtccgc gccagcttca cgggccgcag ccatgaggcc gcggaccgtc
     4741 gagccggcca gcgagcaagg attgggagcg catgtgggcg ttaccggtcg gaagctgtcg
     4801 cctcgcgcga gatcgtcctt gagggcgcgc atgaagacgg cgagccgcgc ttcatgctcc
     4861 ccgcgctgat cgcgcgcctc gcctgacgtg tcgacatagt cgaagtcgat caccgcgacc
     4921 gcggcgcgtt gcgtcttgtt gccaccgtcc tgggccttcc cgcaaaacgg cgccggccac
     4981 aggaagatcg ccagcaccgc aacaagcaaa tgtcgcaccg caactcaccc ctgcttcgcc
     5041 ccgccgatca ctttgcgcgg accgtaggtg gccggaaagc gaacgcgtcc ctgatatggc
     5101 cgggcaaatt gcccgatttt tccgcaggcg cttccaatag gcccgctctg cccctagatt
     5161 gcgcctcgcc cgcgccgttg aatgcaccct ccctttgaaa aggtgttcgg ccatgcccgc
     5221 gcttaaggtc gatctgaaac ttcggctctc gctgcgtgtc gccgcgttgg cggcgctctg
     5281 tttcgccgcc gccgcggctt acgctctgtt cgaaacggac cgctccacgc gggaacgggc
     5341 ggattcaatc gccgaactgg tcgccaggga cctcgcgctg caacagaacc agagcagctg
     5401 ggtcaggggg gcgcccattc agttccctga tttacagcga gtagcggcgg cgctgatggc
     5461 gcccggcctc tgcatcgcct atcgcgcgcc aagcggcgaa attgttcagc gactgtgcgg
     5521 cggcgtcgcg cccgacgata ccagcgcgcc gcggctgttc gccgatctct accagcgcgt
     5581 tttcgatgcg ggccgcgagt cggcgcgtcc ggttggctac ggcaaggaaa cgcacggcga
     5641 ggcggtggcg tcgatcgatc gccaaagcct catcgcacaa agctggcgtg agacgagccg
     5701 cctcgccgcc atcatggccg gcgcgcttgt cgggctttgc gtgctggttt acgcaacgct
     5761 cgcagacgcc ttgcggccga cgcgggcgat ccgcgcgggt cttgagcgcc tcgccgccgg
     5821 cgacctttcg gcgcgcctgc cgccgttcga tctcgccgaa ctgtcggccg tcggcaatgt
     5881 tttcaatcat ctggccggca gccttcagac gacgctttcg gagcgcaacg cgctgacgcg
     5941 acggctgatc gtggt
//
LOCUS       DASWGM010000516         5121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2417374,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000516 DASWGM010000000
VERSION     DASWGM010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417374"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VGA15_15010"
     CDS             complement(<1..393)
                     /locus_tag="VGA15_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9589043.1"
                     /translation="MPTIAETAPVSKPALWMGRVLSGLVIVFMLFDGAIKLVPWPVVT
                     ETMDRIGYGSSETLARSLGVMTLVCTILYAIPPTSILGAILLTGYLGGAMASHLRIGS
                     PLFSHILFGFYLGLMVWGGLWLRDRSLRG"
     gene            complement(435..878)
                     /locus_tag="VGA15_15015"
     CDS             complement(435..878)
                     /locus_tag="VGA15_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9589044.1"
                     /translation="MNLQSSKTIDETKIGTLIDQRVTALRAKDAQAVLSCLAADFVAF
                     SLAPPLISTMTDAKAYEAWFATWQGPIGTEIRDLAIEVSGDLGVSHSLNLMTGTAASG
                     HEVKLWYRQTLSFRKIDGAWKIAHEHTSVPFHMDGSFRAAIDLKP"
     gene            complement(955..2229)
                     /locus_tag="VGA15_15020"
     CDS             complement(955..2229)
                     /locus_tag="VGA15_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX9589045.1"
                     /translation="MTDTAWIDTALSSARPQAVGALLRYFRNLDTAEEAFQNACLRAL
                     KSWPQNGPPRDPAAWLIMVGRNVAIDDIRRSRKQEPLPEDEAISDLDDAEEALAERLD
                     GSHYRDDILRLLFICCHPELPATQQIALALRIVSGLTVKQIARAFLVTEAAMEQRITR
                     AKARVADADVPFETPGAIERAERLSAVAAMIYLIFNEGYSASGDTAEIRSSLCEEAIR
                     LARLLLRLFQSEPEIMGLTALLLLQHARAAARFDAEGAFVLLDDQDRSLWNQKMIAEG
                     LALIDKAMRHRRSGPYQIQAAIAALHARAGKPEDTDWTQIDLLYGALEIMQPSPVVTL
                     NRAVAVSKVRGAEAALELIEPLASRLSNYFHFFGVRGAFLMQLGRNDEARVAFDRAIA
                     LANTSAEAAHIRMHLDRLMRDSQPRGSKISKK"
     gene            complement(2226..2594)
                     /locus_tag="VGA15_15025"
     CDS             complement(2226..2594)
                     /locus_tag="VGA15_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9589046.1"
                     /translation="MLYAILCYHDEDTVGSWTKEQDAAVMKKLAVVQGGLTKQGRLGP
                     VARLLPTTSATTLRKDDPPLVIDGPYAETKEQLLGFYIVDCNNLDEALDVARDLGAAN
                     PGGAYEIRPVGMMTPGSLKT"
     gene            2723..3688
                     /locus_tag="VGA15_15030"
     CDS             2723..3688
                     /locus_tag="VGA15_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEX9589047.1"
                     /translation="MNKGALALIVKGGSANWSPERWKSRFDDVCGDRRVVLLPDAPFD
                     PADIHYAAVWKPGHGELAAFPKLRVIFNLGAGVDALMADSSLPKVPLVRVAVGDLTGR
                     MTEYVVLHVLMHHRQEPYLRACQREKRWEPRSQWAANAISVGVMGLGMLGADAADALR
                     RVGFRVSGWSRNPRQIDGIDCYHGVAQLDTFLQRTDILVCLLPLTPDTRHILNRELFA
                     KLNRNSPIGAPVLINAGRGGLQNEADILQCLDDGTLGAASLDVFADEPLPPDSPFWTH
                     PNVVLTPHNAADTDPDEISKYVARQIERFEAGSALENVVDPARGY"
     gene            complement(3744..4406)
                     /locus_tag="VGA15_15035"
     CDS             complement(3744..4406)
                     /locus_tag="VGA15_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9589048.1"
                     /translation="MVQKNKRPPPLGAPEPVAPKRRGRPRAYQPEIALGKALDLFRKD
                     GFAATSLDDLSAATGMNRPSLYGAFGDKRELYLKSYARYRADARAAMIDIFRDELPIR
                     KRLGRIYAVALDIYLSGDAGPRGCFTVMTAASEAISDPEIRAMVLEGLSELDKAFAAC
                     FRLARENGELPASADPQVLAQLASATIHTIAIRARARVPRKELEAIVTGAIEVMVGAA
                     TK"
     gene            4504..>5121
                     /locus_tag="VGA15_15040"
     CDS             4504..>5121
                     /locus_tag="VGA15_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX9589049.1"
                     /translation="MDLYFSPLACSLATRIALYEAGAEARYLEVDPKTKVVQNDGSDF
                     YQVNPLGLVPTVRTDDGLVLTENAAILQYVADRFPQAGIATGPGMERSRLHQWLCFIG
                     TELHKGLFVPVLDKKAPAEVKTYILGKGLSRLDYLETYLNGREFLLDHFSVADAYLVT
                     VINWTMATPPIELAKWPAVKAYYERLRARPSIAKAVAEEFKLYQAE"
BASE COUNT          934 a         1603 c         1636 g          948 t
ORIGIN      
        1 cccgcgcaag ctcctgtcgc gcaaccacag cccgccccac accatcaggc cgagataaaa
       61 cccgaacagg atgtggctga acagcgggct gccgatccgc agatgcgatg ccatcgcgcc
      121 gccgagatat ccggtcaaga ggatcgcgcc aagaatcgag gtcggcggga tcgcgtagag
      181 aatggtgcag accagcgtca tgactccgag gcttcgcgcc agagtttcgc tcgaaccata
      241 gccgatcctg tccatcgttt ccgtgacgac gggccacggc accagcttga tggcgccgtc
      301 gaacagcatg aacacaataa ccagaccgct gagaacgcgg cccatccaga gcgcgggttt
      361 cgagaccggg gcggtctcag caatcgtcgg catggctctc tccttgagcg gctggttgtc
      421 gggaatctct gacgttacgg cttcagatcg atcgcggcgc ggaagctgcc gtccatatgg
      481 aacggcaccg aggtgtgctc gtgcgcgatc ttccaggcac cgtcgatctt gcggaagctc
      541 agggtctggc ggtaccagag cttgacctcg tgtccgctcg ctgcggtgcc ggtcatcaaa
      601 ttgaggctgt ggctgacgcc aagatcgccg ctcacttcaa ttgcgaggtc gcggatttcg
      661 gtgccgatcg gtccctgcca cgtggcaaac caggcctcgt acgccttcgc gtcggtcatc
      721 gtcgagatca gcggcggcgc cagcgagaac gcgacgaaat ccgctgcgag acacgacagc
      781 accgcttggg cgtctttcgc gcgcagcgcc gttacgcgtt ggtcgattaa ggtaccgatt
      841 ttggtttcgt cgatggtctt gctgctttga aggttcatcg cgcgtctcct tcgatggatt
      901 ggtgatgcac aatccaagga cgaacggggt gggcgcgatc cgacagaaag cgaattattt
      961 tttcgagatt ttggaaccgc gcggctggct gtcgcgcatc aggcggtcga gatgcatccg
     1021 gatgtgggcg gcttcggcgg aagtgttggc gagcgcgata gcgcggtcga atgccacgcg
     1081 ggcttcgtca ttgcggccga gctgcatcag gaaggcaccg cgcacaccga agaaatgaaa
     1141 ataattcgaa agccgcgacg ccagcggctc gatcagttcg agcgccgctt cggccccgcg
     1201 caccttggac accgccaccg cacggttgag cgtcaccacc ggcgacggct gcatgatctc
     1261 gagcgcgcca taaagcagat cgatctgggt ccagtcggtg tcttcgggct tgccggcgcg
     1321 ggcgtgcaat gccgcgatcg ccgcctggat ctgatagggc ccgctgcggc gatgccgcat
     1381 cgccttgtcg atcagcgcaa gtccttcggc gatcatcttc tgattccaca gcgagcgatc
     1441 ctgatcgtcg agcagcacga atgcgccttc tgcgtcaaag cgcgccgccg cgcgggcatg
     1501 ctgcagcaac aatagcgcgg tcagccccat gatctcgggc tcgctctgga acagccgcag
     1561 caacaggcgc gccaggcgaa tggcttcctc gcacagggag ctgcggatct cggcggtgtc
     1621 gccgctcgcc gaatagccct cgttgaagat caggtagatc atcgcggcga ccgcggagag
     1681 ccgctcggcg cgctcgatgg cgcccggcgt ctcgaacggc acgtcggcgt ccgcgacgcg
     1741 ggctttggcg cgggtgatgc gctgctccat tgccgcttcc gtaaccagga acgcgcgcgc
     1801 aatctgtttc acggtcagac ccgaaacgat acgcagcgcc agcgcgatct gctgcgtcgc
     1861 cggcagctcg gggtggcagc agatgaacaa gagccgcaaa atgtcgtcgc ggtagtgcga
     1921 accgtcgagc cgttcggcca gcgcctcctc ggcgtcgtca agatcggaga tcgcctcgtc
     1981 ttcgggtagc ggctcctgct tgcggctgcg ccggatgtca tcgatcgcga cattgcggcc
     2041 gaccatgatc agccacgcgg cgggatcgcg cggcggcccg ttctgcggcc agcttttcag
     2101 cgcccgcagg caggcgttct gaaaggcttc ctcggcggtg tcgagattgc ggaaataacg
     2161 caacagcgcg ccgaccgcct ggggacgcgc cgagctcagc gcggtatcga tccaggcggt
     2221 gtcggtcacg tcttgaggct ccccggtgtc atcatgccga cgggacggat ttcataggcg
     2281 ccgccgggat tggccgcgcc gagatcgcgc gcgacatcca gcgcctcgtc gagattgttg
     2341 cagtcgacga tatagaagcc gagcagctgt tccttggttt cggcataggg cccatcgatc
     2401 accagcggcg gatcgtcctt gcgcagcgtg gtcgccgacg tcgtcggcaa cagccgcgcc
     2461 accggcccga gccggccttg tttggtcagt ccgccctgca cgacggcaag cttcttcatg
     2521 accgcggcgt cctgttcctt ggtccaggaa ccgacggtgt cttcatcgtg gtagcaaagg
     2581 atggcgtaaa gcatgagggg ctcctgttga ttggaggacg aatgagtatg cccgtggccg
     2641 acaggcaagc gaaatatttt gcgccgacac gccaggccga tatggtcgcc cgcagatccc
     2701 gcaactatga ggctaaaatc cgatgaacaa aggagcgctc gccctgatcg tgaagggcgg
     2761 atccgccaac tggtcgcccg aacgatggaa aagccggttc gacgatgtct gcggggatcg
     2821 tcgggttgtc ctgttgccgg atgcgccatt cgatcccgca gacatccatt acgccgcggt
     2881 ctggaagccg ggccacggcg agcttgcggc atttccaaag ctgcgcgtga ttttcaacct
     2941 cggcgcgggc gtcgatgcgc tgatggccga ttcgagcctg cccaaagtgc ccctggttcg
     3001 cgtggcggtc ggcgatctca cgggtcgaat gaccgaatat gtcgtgcttc acgtgctgat
     3061 gcatcatcgg caggagcctt accttcgagc ctgccagcgc gagaagcgct gggagccaag
     3121 gtcgcaatgg gcagcgaacg ctatttcggt cggcgtcatg ggccttggca tgcttggggc
     3181 ggatgccgct gatgcgctgc gacgcgtggg ctttcgcgtg tcgggctgga gcagaaatcc
     3241 ccggcagatc gacggcatcg actgctatca cggcgtggcg caacttgata cgtttctgca
     3301 acggaccgat atcctcgtct gtctgctgcc gctcacgccg gatacgcgac acattttgaa
     3361 ccgggaattg ttcgcaaaac tcaatcgaaa cagcccgatc ggtgcgcccg tcctgatcaa
     3421 tgccggtcgc ggcggcctgc agaacgaggc cgacattctg caatgccttg acgatggcac
     3481 gctcggcgcc gcctcgctgg atgtcttcgc cgatgaaccg cttccgcccg acagcccgtt
     3541 ctggacccat ccgaacgtgg tgctgacgcc gcacaacgcc gccgataccg atcccgacga
     3601 gatctcgaaa tacgtggcgc gccagatcga acgttttgaa gccggcagcg cgctcgagaa
     3661 tgtcgtcgat cccgcgcgcg gatattagcg gcctatcgtc gtccccgcgc aggcgggttg
     3721 gcttggccgg gacgacgcgg agatcatttc gtcgccgccc ccaccatcac ttcgatcgca
     3781 cccgtcacga tcgcctccaa ttccttgcgc ggcacgcggg ctcgcgcccg gatcgcgatg
     3841 gtgtggatgg tcgcggacgc aagctgcgcc agcacttgcg gatcggctga tgcgggcaac
     3901 tcgccgttct ccctggcaag ccggaagcag gccgcgaacg ccttgtcgag ttcggaaagt
     3961 ccctccagca ccatggcgcg gatctcgggg tcggagatcg cttcggaggc cgccgtcatc
     4021 accgtgaagc agccgcgcgg gccggcgtcg ccggacagat agatatcgag cgccaccgcg
     4081 tagatgcgcc ccagccgttt gcggatcggc agttcgtccc tgaaaatatc gatcatcgcc
     4141 gcgcgcgcgt cggcgcgata gcgcgcgtaa ctcttgaggt agagctcgcg cttgtcgccg
     4201 aacgcgccat agaggctcgg ccggttcatg ccggtggcgg cgctgagatc gtcgagcgag
     4261 gtggcggcga aaccgtcctt gcggaacaga tcgagcgcct tgccgagcgc gatttcgggc
     4321 tgataggcgc gcggccggcc gcggcgtttt ggcgcgacag gctcgggtgc acccagcggc
     4381 ggcggtcttt tatttttttg taccatttcg caataaattc cttgacgcga tttatattat
     4441 gcggaacagt atgaaaatca accccggttc aacccaactg aaccgaaagc caaggaggca
     4501 tccatggacc tgtatttctc gccgctcgcc tgctcgctgg cgacccgaat tgcgctgtat
     4561 gaggccggcg ccgaggccag atatctcgag gtcgatccga agacgaaggt cgtgcagaac
     4621 gacggctcgg atttttatca ggtgaacccg ctcgggctgg tgccgacggt gcgcaccgat
     4681 gacgggctcg ttctcaccga gaacgcggcg atcctgcaat atgtcgcgga tcgctttccg
     4741 caggccggca tcgcaaccgg ccccggcatg gaacgcagcc ggttgcatca atggctctgc
     4801 ttcatcggca ccgaactgca caagggtttg ttcgtgccgg tgctcgacaa gaaggcgccg
     4861 gccgaggtga agacctacat cctcggcaag ggcctgtcgc ggctcgatta tctggaaacc
     4921 tatctcaacg gccgcgaatt cctgctcgac catttcagcg tcgccgacgc ttatctggtg
     4981 acggtcatca actggaccat ggcgacgccg ccgatcgaac tggcaaaatg gccggcggtg
     5041 aaagcctatt acgagcgcct gcgcgcccgc cccagcatcg ccaaagcggt cgcggaagaa
     5101 ttcaagctgt accaggccga a
//
LOCUS       DASWGM010000517         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2136421,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000517 DASWGM010000000
VERSION     DASWGM010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2136421"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VGA15_15045"
     CDS             <1..612
                     /locus_tag="VGA15_15045"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEX9589050.1"
                     /translation="RDQPEAGAMSFTRQQAPHGLGHAVWCARDIVGNEPFAVVLPDEL
                     VLNTPGCLKQMIDAAGKLGDKSNLIAVEEVPAHLTHQYGICGVGKRHKHASMFEIDGM
                     VEKPPKGTAPSNLSITGRYILQPEIFKILETQERGAGGEIQLTDAMIGLAKTQSFYGV
                     EFEGERHDCGSKAGFLRANIAFGMARADLRDDLRAEMKKYLEG"
     gene            740..1078
                     /locus_tag="VGA15_15050"
     CDS             740..1078
                     /locus_tag="VGA15_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9589051.1"
                     /translation="MQTVVETPSYLADAERLFSPEERLAVVDRLAADPTCGVVIPGSG
                     GIRKVRFGFGARGKSGGARIIYLFSGDRLPVFVLAVFAKNEKANLSQAERNALAKMVI
                     DMIGSYRRQK"
     gene            1075..1377
                     /locus_tag="VGA15_15055"
     CDS             1075..1377
                     /locus_tag="VGA15_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9589052.1"
                     /translation="MTKAAFDKIKAGLGEAKAYLDGSGNKRGYGIHVPARVNVKKIRT
                     RLGLSQESFAQTYGFALSAVRDWEQGRRQPERSARILLRVVEKEPEAVTRALAKSA"
     gene            1397..1645
                     /locus_tag="VGA15_15060"
     CDS             1397..1645
                     /locus_tag="VGA15_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589053.1"
                     /translation="MNDYDPLRDYLKKQTLPEFVLSFEKIEEIIDAALPRAAHRASWW
                     ETLRSPQEKMPQREACLAGGYIATRLADGRSVRFKRVK"
     gene            complement(1795..>2175)
                     /locus_tag="VGA15_15065"
     CDS             complement(1795..>2175)
                     /locus_tag="VGA15_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9589054.1"
                     /translation="MENPEHAAQMLTRIRELGTGLSLDDFGTGHSSLAYLQRFPFDTI
                     KIDQSFVRTSSRGTRPVILKSIIAMAHDLGMDVVAEGAETDSDAVELYQLGCEYAQGF
                     AFGEPMDADAAMRLLTEERLEAAS"
BASE COUNT          439 a          647 c          701 g          388 t
ORIGIN      
        1 cgcgatcagc ccgaggcggg cgccatgagt ttcacgcggc agcaggcgcc gcacggcctc
       61 ggccacgcgg tgtggtgcgc ccgcgacatc gtcggcaacg agccgttcgc ggtggtgctg
      121 ccggacgaac tggtgctgaa cacgccgggc tgcctcaaac agatgatcga cgccgccggc
      181 aagctcggtg acaaatccaa cctgatcgcg gtggaagaag tgccggcgca tctcacccat
      241 cagtatggca tctgcggcgt cggcaagcgc cacaaacacg ccagcatgtt cgagatcgac
      301 ggcatggtgg agaagccgcc caagggtacc gcgccgtcca atttgtcgat caccgggcgc
      361 tacattctgc agccggaaat tttcaagatc ctggaaaccc aggagcgcgg cgccggcggc
      421 gagatccagc tcaccgatgc gatgatcggg ctcgccaaaa cccaaagctt ctacggcgtc
      481 gaattcgagg gcgagcggca cgattgcggc tccaaggccg gcttcctccg cgccaatatc
      541 gccttcggca tggcgcgcgc cgatctgcgc gacgacttgc gcgcggagat gaagaagtat
      601 ctggaggggt gagattcacg ccattccggt tgcgctaacc ctgccttccg cttctccctt
      661 ccttttgctt ggataatcga ggtcggatca aacatcgaag cttgatagta ctacggcaat
      721 gccgtagtat gcaggttcga tgcagaccgt cgtggaaacg ccgtcttatc tcgccgacgc
      781 ggagcggctg ttttcgccgg aagaacgctt ggccgtcgtg gatcgtctag ccgctgaccc
      841 cacatgcggc gtggtcattc ccggcagcgg cggcattcgc aaggtgcggt ttggcttcgg
      901 tgcgcggggc aagagcggcg gcgcacgtat tatctatctg ttcagtggag acaggctccc
      961 cgtgtttgtt ctggcagttt ttgccaaaaa cgagaaagcc aacctttcgc aagccgagcg
     1021 taatgctctg gccaagatgg tcatcgatat gatcggaagt taccggaggc aaaaatgaca
     1081 aaagccgcat tcgacaagat caaagccggg ctcggcgagg ccaaagccta tctcgatgga
     1141 tccggaaaca agcgtggcta cgggattcat gttccggcgc gggtgaacgt gaaaaaaatc
     1201 cgcacccggc ttggcctctc gcaagagagt tttgcacaaa cctacgggtt tgcgctgtcg
     1261 gctgtacgcg attgggagca gggccgccgc caacctgaac gcagcgcgcg catcctgctc
     1321 agggtcgtcg aaaaggagcc ggaggcggta acgcgggccc tggcgaaaag cgcatgaccg
     1381 ttgaccgaga ccgcccgtga acgattacga cccgctgcgc gattatctga agaagcagac
     1441 gctgcccgaa ttcgtgctga gcttcgagaa gatcgaggag atcatcgacg cggcgctgcc
     1501 gcgcgcggcg catcgcgcgt catggtggga gaccttgcgc agcccgcagg aaaagatgcc
     1561 gcagcgcgag gcctgtcttg ccggcggcta catcgcaaca cggctcgcgg acggcaggag
     1621 cgtgcggttc aagcgggtga agtgagaaga gtggagcggc gcagcgtcac aacacgcact
     1681 gccgtcatac gcgggcttga cccgcgtatc catcaatctt tgcaaaggag atggattgcc
     1741 gggtcatcta gcgcgaagac gcgcttcgcg ctgttgcccg gcaatgacga agggtcagct
     1801 cgccgcctcg agccgttctt cggtgagtag ccgcatcgcg gcgtcggcgt ccatcggctc
     1861 gccgaaggca aagccctggg cgtattcaca gccgagctga taaagctcca ccgcgtcgga
     1921 atcggtttcc gcgccttcgg ccaccacgtc catgccgagg tcgtgggcca tggcgatgat
     1981 cgatttgagg atcaccggcc gggtgccgcg gctagaggtg cgtacgaacg actggtcgat
     2041 cttgatggtg tcgaacggaa agcgctgcag ataggccagc gacgaatggc cggtgccgaa
     2101 atcgtccagc gacagcccgg tgccgagttc gcggatccgc gtcagcatct gcgctgcatg
     2161 ttccgggttc tccat
//
LOCUS       DASWGM010000518         3930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1560390,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000518 DASWGM010000000
VERSION     DASWGM010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560390"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1314)
                     /locus_tag="VGA15_15070"
     CDS             complement(64..1314)
                     /locus_tag="VGA15_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX9589055.1"
                     /translation="MRYSLTSLLVNALAGHRGWKPQWRDAEPRDSYDVVIVGGGGHGL
                     STAYYLARECGITDVAVLEKAWIGGGNVGRNTTIIRSNYLLPGNIPLYELSMKLWEGL
                     EQDFNYNAMISQRGVLNLYHSDGQRDAYARRGNAMRIHGVDAELLDQDGVRRLYPFLN
                     FDSARFPIQGGLLQRRGGTVRHDAVAWGYARGASDRGVDIVQNCEVTGFSITDGRVTG
                     VQTSRGSIRANKVAIAVAGSSSRVAAMAGMRLPIESHVIQAFVSEGLKPIIPGVITFG
                     AGHFYISQSDKGGLVFGGDIDGYNSYAQRGNLPVVEDVCEGGMALMPAIGRARILRNW
                     GGLLDMSMDGSPIIDRTPTQGLYLNAGWCYGGFKATPGSGFAFAHLIARDAPHPAAIH
                     LRLDRFRSGHLIDEKGQGAQPNLH"
     gene            1416..2360
                     /locus_tag="VGA15_15075"
     CDS             1416..2360
                     /locus_tag="VGA15_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEX9589056.1"
                     /translation="MSRPHRFGFLLIDDFALMSFASAVEPLRAANILAGRTLYEWFHI
                     STGAGAIAASNGLSVTADHTISDKIRFDTVLVCAGGNPASFDDARAIAWLRRLARSGC
                     AIGGVSGGPFILAKAGILDGVRCTIHWEHLPAFTEAFPDLVSTRTLFEIDRGRLTCGG
                     GVAALDMMHALIRRDHGQSLAARVSDWYLQTAVRLGDSTQRISLLERVGTNNRSLIAA
                     IGSMERQLASPMSRPALAKAAHVSVRQLERLFAKHLGVTIGQHYLALRLTKAQALLRQ
                     TSLPIVQIGAECGFANASHFARVYRQRFGTLPSKDRMR"
     gene            complement(2504..3412)
                     /locus_tag="VGA15_15080"
     CDS             complement(2504..3412)
                     /locus_tag="VGA15_15080"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9589057.1"
                     /translation="MQINRSVLRGSYANIGDYVALTKPRVMSLVVFTALVGLMVAPGG
                     IDPFAGVVALLCIAAGAGAAGALNMWYDADIDAVMARTAMRPIPSGRVSRSEALVFGL
                     MLGMCAILALGALLNMAAAALLAFTIFFYVVVYTMWLKRRTPQNIVIGGAAGALPPVI
                     GWVAAAGNVGLEPLILFLIIFLWTPPHFWALSLNLAGEYARAGVPMLPVVAGKAETKR
                     QILLYSVVLVPISLIPWALGFAGAIYGAAAAMLGAIMIFLSWQVRRSNDGERRPARRL
                     FVFSMLYLVLLFGVLLMNAAPYAQSH"
     gene            complement(3590..3925)
                     /locus_tag="VGA15_15085"
                     /pseudo
     CDS             complement(3590..3925)
                     /locus_tag="VGA15_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
BASE COUNT          814 a         1184 c         1202 g          730 t
ORIGIN      
        1 gagatagctg aattcggcgg cgtcgcgaag tccacaaaac gggcagggga tacgcatggc
       61 gcctcaatgc aggttcggtt gggcgccctg gcccttttcg tcgatgagat gcccggagcg
      121 aaatctgtcg agccggagat gaatggccgc cggatgcggc gcgtcgcgcg cgatcagatg
      181 ggcgaaggca aatccggatc cgggcgtggc cttgaagccg ccgtagcacc agccggcgtt
      241 gagatagagg ccttgcgtcg gcgtgcggtc gatgatcggc gagccgtcca tcgacatgtc
      301 cagcaggccg ccccagtttc ttaagatgcg cgcccgtccg atcgccggca tcaacgccat
      361 gccgccttcg cagacatctt ccaccacggg aaggttgccg cgctgggcat aggagttgta
      421 gccgtcgatg tctccgccga acacgagccc gcccttgtcg gactggctga tgtagaaatg
      481 ccccgcgccg aaggtgatga cgcccggaat gatcggcttc agcccttccg agacgaaggc
      541 ctggatgaca tggctctcga tcggcaaccg cattccagcc atggcggcga cgcgcgaact
      601 gctgccggcg accgcgatcg caaccttgtt ggcacggatc gatccgcgcg aagtctgcac
      661 gccggtgacg cggccatcgg taatggaaaa gccggtgact tcgcagttct gcacgatatc
      721 gacaccgcgg tcgctcgcac cacgggcgta gccccatgcc accgcgtcgt gccggaccgt
      781 gccgccgcga cgctgcaaca ggccgccttg aatcggaaac cgggcagagt cgaaattgag
      841 aaagggatac agtcttcgca cgccgtcctg atcgagcagt tcggcgtcaa ccccgtgaat
      901 ccgcatcgcg ttgccacgcc gcgcataggc gtcgcgctgg ccgtcggaat gatacaggtt
      961 cagaacgccg cgctggctga tcatcgcgtt gtaattaaaa tcctgctcca gtccttccca
     1021 cagcttcatc gagagttcgt aaagcgggat gttgccgggc agcaaatagt tggagcggat
     1081 gatcgtggtg ttgcgcccga cattgccgcc gccgatccat gccttttcaa gcaccgcgac
     1141 gtcagtgatg ccgcactccc tcgcgagata ataggccgtc gaaagaccat gaccgccgcc
     1201 gccgacgatg accacgtcat agctgtcgcg aggttcggcg tcgcgccatt gcggcttcca
     1261 gcccctgtgg ccggcgagag cgttgaccag cagggatgtc agcgaatagc gcatggtgcg
     1321 gccgggagag ttggatcgga acgccgactt tcgcgcggaa acgaaaccgg ctatacgctg
     1381 ttagcgacat caaatgttct aaatgcgaca tcgccatgtc ccgtccgcac cgtttcggct
     1441 ttctgctgat cgacgatttc gcgctgatgt cgttcgcgtc cgcagtcgag ccgttgcggg
     1501 cggcgaatat cctggcaggc cggacgctct acgaatggtt tcatatctcg accggcgccg
     1561 gcgccattgc cgcctcgaac ggcctgtcgg tgaccgccga tcacaccatt tccgacaaga
     1621 tacggttcga caccgtgctg gtctgcgccg gaggaaatcc ggcctcgttc gacgatgcca
     1681 gggcgattgc gtggttgcga aggctggcgc gatccggctg cgcgatcggt ggcgtctcgg
     1741 gcggcccatt tatcctcgcc aaggcaggca ttctcgatgg cgtccgttgc accattcact
     1801 gggaacatct gcctgccttc accgaggcgt ttccggatct cgtctccaca cgaacgctgt
     1861 tcgagatcga tcggggacgt ctgacctgcg gcggcggcgt ggccgcgctg gacatgatgc
     1921 atgcgctgat ccgcagggat cacggccagt cgctcgcggc gcgggtcagc gattggtatt
     1981 tgcagacggc ggtccgtctc ggcgacagca cgcagcgcat ctcgttgctg gagcgtgtcg
     2041 gaaccaacaa ccgaagcctg attgccgcga tcggcagcat ggagcggcag ctcgccagcc
     2101 cgatgtcgcg gccggcattg gcgaaggccg cgcacgtgtc ggtgcgccag ctcgaacggc
     2161 tgttcgcaaa acacctcggc gttacgatcg gccagcatta cctggcgctg cgcctgacaa
     2221 aggcccaggc gctgttgcgg cagaccagcc tgccgattgt tcagatcggc gccgaatgcg
     2281 gttttgccaa tgccagccat tttgcgcggg tgtaccggca gaggttcgga acgctgccgt
     2341 caaaggaccg catgcggtga agcggcgcgg gaattgacct gttgaaactg gccctttcaa
     2401 gtggccggta caagacaagg caaggcaagg caaggcaagg caaggcaagg caaggcaagg
     2461 caaggcaagg caaggcattc tatcgcctcg tgaagcgctg gctctagtgg gactgagcgt
     2521 agggcgcagc attcatcagc agcacaccaa aaagaaggac cagatagagc atcgaaaaca
     2581 cgaacaggcg acgagcaggc cgcctttccc cgtcattgct ccgacgtact tgccatgaga
     2641 gaaaaatcat gatcgctcca agcatcgccg cagccgcgcc atagatggcc cctgcgaacc
     2701 ccagtgccca gggtatcagt gagatcggaa ccagaacaac gctatagaga agaatctggc
     2761 gctttgtttc ggccttgccg gccacgaccg gcaacatcgg cacaccggcg cgagcgtatt
     2821 ctccggcaag attgagcgac agtgcccaga agtggggtgg cgtccaaagg aagatgatca
     2881 ggaacaggat gagcggttcg agcccgacat ttccggcggc cgcaacccag ccgatcaccg
     2941 gagggagtgc gccagccgca ccgcctatga cgatattttg cggtgttcgg cgtttgagcc
     3001 acatcgtgta gacgacgaca tagaagaaaa ttgtgaaagc aagcagcgca gccgcggcca
     3061 tgttcagcaa agcgccgagg gccagaatgg cgcacatgcc aagcatcagt ccgaaaacca
     3121 gtgcttccga gcgcgataca cggccgctag gaattggacg catggcggta cgcgccatca
     3181 ccgcatcgat gtcggcgtca taccacatat tgagcgcacc cgcggcgcca gctccggcag
     3241 ctatgcaaag aagagcaacg acaccggcga agggatcgat gccacctggt gcgaccatga
     3301 gacccacgag tgcggtaaag acgaccagcg acataacgcg cggtttcgtc agcgcgacat
     3361 agtccccgat gttggcatag ctcccgcgaa gaactgaacg attgatttgc atgagattaa
     3421 tgcctcgtct tgcaagcgac atcgcgacct ctttcagtca gatattcaaa aaaagccatc
     3481 ggttgtagaa cactcttgtc aggcggtctc cgaaaggcga cgggttcggt ttcactgact
     3541 gcgccgctgc cgcaccttcg cgagttcttc cggcagggac aatccccgcc ttctttcccg
     3601 tagaatcgaa ctgtttgaga taggcgacga tatcgtcaat ttgctttgga tctttcaagc
     3661 ctggaaaagt cattttggta ccaggtatct tggtcctcgg atccctgatg tattcgcgga
     3721 aagttgcttc atcccaggtg attccggaat tcttgttggc gggagaatag ctgtagcctt
     3781 caatcatccc agcctttcgc ccgaagagcc cgttgagcac cgggccaacc gcgttctttg
     3841 cgttctcccc gatttggtga cacgccttgc attggacgaa aatcttctcg cccgaagccg
     3901 catcctgggc atacccgggg acgatcgcga
//
LOCUS       DASWGM010000519         7415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1849968,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000519 DASWGM010000000
VERSION     DASWGM010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849968"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VGA15_15090"
     CDS             complement(<1..348)
                     /locus_tag="VGA15_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEX9589058.1"
                     /translation="MDKTTPHPLFKLATELGPLIVFFVANAKFNLFVATGAFMVAIIA
                     AMMASYVVTRHVPIMALVTGVIVIVFGTLTLVLHDETFIKIKPTIIYGLFAAILGGGL
                     LFGRSFIAIMFDQM"
     gene            complement(481..1422)
                     /gene="ftsY"
                     /locus_tag="VGA15_15095"
     CDS             complement(481..1422)
                     /gene="ftsY"
                     /locus_tag="VGA15_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX9589059.1"
                     /translation="MNDTPETTKLSWWRRLSSGLKRTSSSLGTAVADLVTKRKLDRAM
                     LEDIEDVLLRADLGTEVAARIAAAVGVGRYDKAISADDVKAVVATEVEKVLSSVAKPL
                     EIDQAQKPFVILVVGVNGSGKTTTIGKLAAKLAAEGRKVMLAAGDTFRAAAIEQLKIW
                     GERTKSPVIAGAQGSDSASLAFNALTAAREGKLDVLLVDTAGRLQNKAELMNELEKVV
                     RVIKKVDASAPHAVLLVLDATVGQNALSQVEAFHRTAGVTGLVMTKLDGTARGGILVA
                     LAEKHKLPVHFIGVGEGIDDLAPFTARDFAQAIAGIE"
     gene            1451..2353
                     /locus_tag="VGA15_15100"
     CDS             1451..2353
                     /locus_tag="VGA15_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX9589060.1"
                     /translation="MSESHNARIDVPELTADQIQARVLHRDGLMLVIDKPAGLPVHRG
                     PKGGANLEASFDALRFGLPRPPVLAHRLDRDTSGCLVLGRHRKATASLGLLFKHGRIS
                     KTYWAVVEGGPVEDEGVIDMPLGRLNAERGWWQKPDPEGQTAVTRWKVLGRSFVAAPV
                     VGDVYSPPPCGVETSEARSQGSGVGVPQGQTAVVDPPPQPSPARGEGAVGSSGSTHFT
                     WLALEPVTGRTHQLRVHAASMGWPIVGDNIYGNGPRFGEPSLHLHSREIVIPISRNKE
                     PVRAVAPAPAHLQQRLRACGWNGE"
     gene            complement(2572..3843)
                     /gene="mtaB"
                     /locus_tag="VGA15_15105"
     CDS             complement(2572..3843)
                     /gene="mtaB"
                     /locus_tag="VGA15_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX9589061.1"
                     /translation="MSVDVVTFGCRLNAFESEVIAREAERAGLTDTIVINSCAVTNEA
                     VAQARQSIRRLKRERPEARIVVTGCAAQTQAQMFATMAEVDRVVGNEDKMHSEAWRAA
                     RMALDAAPAFGIASEEKIAVADIMAVKEMAPHLLEGFQKGLPRVFVQVQNGCDHRCTF
                     CIIPYGRGNSRSVPMGAVVSQVRALVERGHTEIVLTGVDLTSYGADLPGAPKLGQLTK
                     QILRHVPELKRLRISSIDQVEVDRDLLDAIADDERLMPHLHLSLQSGDDLILKRMKRR
                     HSRSQAVEFCAQVRRLRPDIALGADIIAGFPTETDEMFARSQGLVEECDLTFLHVFPY
                     SPRPGTPAARMPQVSGEKIKERARRLRAAGKAALQRRLACEIGATRQVLIESATQGRT
                     EHFIPVSISGETPGAVRSLTITGNDGARLTV"
     gene            complement(3845..4717)
                     /gene="dapF"
                     /locus_tag="VGA15_15110"
     CDS             complement(3845..4717)
                     /gene="dapF"
                     /locus_tag="VGA15_15110"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX9589062.1"
                     /translation="MSALANHAFAKMNGIGNEIVVVDLRDRPAVVTAEEARAVASPAG
                     VHYDQLMVLQPPRLPGTEAFIRIYNNDGSEAAACGNGMRCVARRLFENTGQTAATFET
                     KAGLLNCWQGPSGDLYTVDMGPPKFGWKDIPLAEEFRDTRYIELQVGPIDAPVLHSPS
                     VVSMGNPHAIFWVDDVNAYDLGRFGPLLENHPIFPERANITLAHIVDRDHIAIRTFER
                     GAGLTKACGSAACATAVAAARLKRANRIVSISLPGGDLSIEWRERDDHVLMTGTATFE
                     YEGRFDPALFASVA"
     gene            4938..5621
                     /locus_tag="VGA15_15115"
     CDS             4938..5621
                     /locus_tag="VGA15_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX9589063.1"
                     /translation="MNRPRLIRLAMVALIPAAISLGMGSAQAQSPPAATPPPAATPAP
                     APTATPAPPPAASPSAAPPAAAETPAPVQTADPFGEEIILTQKPVVIIKGNANWDSAF
                     DTLIESFKSLSALLDKQGIKPSGNSMIVYTSTDDTGFTYLAEMPVDQDPKNLTKDMSI
                     GKSPEGKALKFVHRGSYDNMDNTYEAITNHLDDKKLEAKDTFIEEYITDPLKTAEDKL
                     VINVYVPLK"
     gene            5627..6328
                     /locus_tag="VGA15_15120"
     CDS             5627..6328
                     /locus_tag="VGA15_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX9589064.1"
                     /translation="MKHLTVLAVAIAAGTLVAAPALAQDIPPAAISVTGEATVSVPPD
                     LAVVDGGVSSEAKTAREASEANSAAMAKVLAALKGAGIDAKDIQTSRLSLQPQSAPNR
                     TGPSAVVGYRASNRVTIRVRDVAKVANVIDTLVGAGANEIGGINFMVSQASKLLDEAR
                     EQAVADARRKAEIYAKAAGVTLGAPLSISEEGSPGPMPFRKMAAGMAVSAPVAQGEET
                     LQVTVSVSWAIKQTQ"
     gene            complement(6461..>7415)
                     /locus_tag="VGA15_15125"
     CDS             complement(6461..>7415)
                     /locus_tag="VGA15_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9589065.1"
                     /translation="DHFDGTHFFDPDGSPPKSLGEVLRWQFGPGRERQAWPEWAPSPH
                     ADTPPPRVTGEKVRLSFVGHASWLIQTSGLNILIDPVWSTRASPLSFAGPKRHNDPGI
                     ASDALPPIDVVLVSHGHYDHLDLATLSKLAASFSPRVITPLGNDVTMRSADSAIKAEA
                     FDWHNRVELGSGIAVTLVPTRHWTARGLFDRNKALWASFVLQTPAGKIYIVCDSGYGE
                     GKHFRRVAEAHGPLRLAILPIGAYEPRWFMRDQHMNPFDAVKALADCGAAQALAHHHG
                     TFQLTDEAIDAPVIALQAALEEARIPRERFAVLKPGQVVEI"
BASE COUNT         1364 a         2433 c         2312 g         1306 t
ORIGIN      
        1 catctggtcg aacatgatcg cgatgaagga gcggccgaac agcaggccgc cgccgaggat
       61 cgcggcgaac aggccgtaga tgatggtcgg cttgatcttg atgaaggttt catcgtgcaa
      121 caccagggtc agcgtgccga acacgatgac gatgacgccg gtgaccagcg ccatgatcgg
      181 cacatgccgc gtcaccacgt aggacgccat catcgccgcg atgatcgcca ccatgaacgc
      241 gccggtggct acgaacaaat tgaacttggc attggcgaca aagaacacga tcagcggacc
      301 gagttcggtc gcgagcttga acagcggatg cggcgtggtc ttgtccatta ttcgatccac
      361 ctaattcatc tcgcccccat ccttcgagac ggcgcttcgc gcctcctcag gatgacggca
      421 ttccggttgg tctctccgtc atcccgagga gcggcgtagc cgcgtctcga aggataagcc
      481 ctactcaatc cccgcgatcg cctgcgcgaa atcccgcgcg gtgaacggtg cgaggtcgtc
      541 gatgccctcg ccgacgccga taaaatgcac cggcagtttg tgtttttcgg ccagcgccac
      601 caggatgccg cctcgcgcgg tgccgtcgag cttggtcatc accaggcccg tcacgcctgc
      661 ggtacgatga aacgcctcga cttgcgacag cgcattttgt cccaccgtgg catccagcac
      721 cagcagcacc gcgtgcggcg ccgacgcatc gaccttcttg atgacgcgaa cgactttttc
      781 aagttcattc atcagttccg ccttgttctg caggcggcct gcggtgtcga ccagcagcac
      841 gtcgagcttt ccttcccgcg ccgcggtgag ggcattgaag gcaaggctcg cggaatccga
      901 accctgcgcg cccgcgataa ccggcgattt ggtacgctcg ccccagatct tcaactgctc
      961 gatcgcggcg gcgcgaaagg tgtcgccggc ggccagcatc accttgcggc cttcggcggc
     1021 aagtttggcc gcgagcttgc cgatggtcgt ggtcttgccg gaaccgttga cgccgaccac
     1081 gagaatgacg aacggctttt gcgcctgatc gatctcaagc ggcttcgcca cagatgacaa
     1141 gaccttctcg acctcggtcg cgaccacggc tttgacgtcg tcggccgaga tcgccttgtc
     1201 gtaacggccg acgccgaccg cggcggcgat gcgcgcggcc acctcggttc ccagatcggc
     1261 gcgcagcaac acgtcctcga tatcctcgag catcgcgcga tcgagcttgc gcttggtgac
     1321 gagatcggcc accgcggtcc caagcgaact cgacgtgcgc ttcaggccgc tggacagccg
     1381 ccgccaccag ctcagctttg tcgtttccgg agtgtcgttc atggcggggg tgtgttagct
     1441 gtttcgcgcc atgagcgaaa gtcacaacgc gcgcatcgat gttcccgagt taaccgccga
     1501 tcaaattcag gcgcgcgtgc tccatcgcga cggattgatg ctggtgatcg acaagccggc
     1561 ggggctgccg gtacatcgcg gccccaaggg cggcgccaat ctggaggctt ccttcgatgc
     1621 gctgcggttc ggcctgccgc ggccaccggt tctcgcccac cgcctcgacc gcgacacttc
     1681 cggctgcctc gtgctgggac gccaccgcaa ggcaaccgcg tcgctggggc tgttgttcaa
     1741 gcacggccgg atttccaaga cctactgggc ggtggtcgaa ggcggcccgg tggaggacga
     1801 gggtgtgatc gacatgccgc tcggccgcct caacgccgag cgcggctggt ggcagaagcc
     1861 cgatccggaa ggccagacgg cggtcaccag gtggaaagtg ctgggacgtt cttttgtcgc
     1921 cgctcccgtc gtcggagacg tttactcccc tcccccttgc ggggtcgaga cgagcgaagc
     1981 tcgctcacag gggtcggggg tgggggtgcc ccaaggacaa actgccgttg tcgacccccc
     2041 tccccaaccc tcccccgcaa ggggggaggg agcagtcggt tcaagtggct caactcattt
     2101 cacctggctc gcgctcgaac cggtgaccgg ccgcacccat caattgcgcg ttcacgcggc
     2161 ctccatgggc tggccgattg tcggcgacaa tatctatgga aatggaccgc gcttcggcga
     2221 gccgagcctg catctgcatt cccgcgagat cgtgattccg atttccagga acaaggagcc
     2281 ggtgcgcgca gtggcgccgg cgccggcgca tttgcagcaa cggctgcggg cctgcggatg
     2341 gaatggggaa tagcgtcgct cgctttcttc ccctctcccc ttgtgggaga gggtggcttc
     2401 gcgaagcgaa gacgggtgag gggtctcttt ctaccgatag gccgtaaccc ctcatccggc
     2461 acggacttcg catagccgat gcaaagcatc ggcgtcctct taaaggacgg cggccgaagg
     2521 ccgcctatgg ccaccttctc ccacaaggga agaaggaagg aacagccaaa ctcacaccgt
     2581 caatcgcgcg ccgtcattcc cggtgatcgt gagcgaccgc accgcgcccg gcgtttcgcc
     2641 gctgatggag actggaataa aatgctcggt gcggccctgt gtggcgctct cgatcagcac
     2701 ctggcgcgtt gcgccgatct cgcaggccag ccgccgctgc aaagcggcct tacccgccgc
     2761 gcgcaatcgc ctggcgcgtt ccttgatctt ttcgcccgat acctgcggca tccgcgccgc
     2821 cggcgtgcca ggccgcggcg aatagggaaa gacatgcaaa aaggtgaggt cgcattcttc
     2881 caccaaccct tgggaacgcg cgaacatttc gtccgtctcg gtcggaaacc ccgcgatgat
     2941 atcggcgccg agcgcgatgt ccggccgcaa tcggcgcacc tgcgcgcaga attccaccgc
     3001 ctggctgcgg gaatgccggc gcttcatccg cttcaggatc agatcgtcgc cggattgcag
     3061 cgacagatgc agatgcggca tcaatcgttc atcatccgcg atcgcgtcga gcagatcgcg
     3121 atcgacctcg acctgatcga tcgatgagat tcgcagccgt ttcaattccg gcacatgccg
     3181 caggatttgt tttgtcagct ggccgagttt tggcgccccc ggcaggtcgg cgccatagct
     3241 cgtcagatcg accccggtca gcacgatttc ggtatggcca cgctcgacca gcgcgcggac
     3301 ctggctgacc accgcgccca tcggcaccga acgcgaattg ccgcggccat aggggatgat
     3361 gcagaacgtg cagcggtgat cgcagccgtt ctgcacctgc acgaataccc gcggcaggcc
     3421 tttttgaaat ccctccaaga gatgcggcgc catctccttg accgccatga tgtcggcgac
     3481 cgcgatcttt tcctcgctgg caatgccgaa tgccggcgcc gcgtccaagg ccatgcgtgc
     3541 ggcgcgccat gcctcgctgt gcatcttgtc ttcattgccg acgacgcggt cgacttctgc
     3601 catcgttgcg aacatctggg cttgcgtctg cgctgcgcag ccggtcacca cgatccgagc
     3661 ttccggccgc tcgcgcttca gccgccggat cgattgccgc gcctgcgcca cggcttcgtt
     3721 ggtcaccgcg caactgttga tgacgatggt atcggtgagc ccagcgcgct cagcctcccg
     3781 cgcaatcact tcggattcga acgcgttgag gcggcagccg aaggtgacga cgtcaacgct
     3841 catctcaggc gacgctggca aataatgccg gatcgaatcg cccctcatac tcaaacgtcg
     3901 ccgtgcccgt catcaagacg tggtcgtcgc gctcacgcca ttcgatggaa aggtcgccgc
     3961 cgggcagcga aatgctgacg atgcgattgg cgcgcttcag ccgcgccgca gccacagcgg
     4021 tggcgcaggc cgccgagcca caggcttttg tgagtcccgc gccgcgctca aaggtgcgga
     4081 tcgcgatatg gtcgcgatcg acgatatgcg ccagcgtaat attcgcgcgc tccggaaaga
     4141 tcggatggtt ttccaacagc ggcccgaagc ggccgagatc gtaggcgttg acatcgtcga
     4201 cccagaagat cgcatgcgga ttgcccatgc tgaccaccga cggcgaatgc agcaccggcg
     4261 cgtcaatcgg cccgacctgc aattcgatat agcgggtatc gcgaaactct tccgcgagcg
     4321 gaatatcctt ccagccgaat ttcggcgggc ccatgtcgac ggtgtagaga tcgccggacg
     4381 gaccctgcca gcaattcaac agcccggctt tggtctcgaa ggtggcggcg gtctggccgg
     4441 tgttctcgaa cagccggcgc gccacgcagc gcatgccgtt gccacaggcc gccgcttccg
     4501 aaccgtcatt gttgtagatg cggatgaagg cctcggtgcc gggcaggcgc ggcggctgca
     4561 gcaccatcag ctggtcgtag tgcacgcccg cgggcgaggc caccgcgcgc gcctcctcag
     4621 ccgtgacgac ggcgggcctg tcgcgcagat cgaccacgac aatctcgttg ccgatgccgt
     4681 tcatcttggc aaacgcatga ttggcgagcg cgctcatgga atcttccaga ctggtgcctg
     4741 tcttatatgg cgaacgaggg cggcttggcc agtgcggcaa atctgtgtcg gctccctgct
     4801 ggcagcggag gcccatgaaa tatctgtcat gggactaatg cagccctcgc gtgttagaat
     4861 ggcaacgtct tcgccggcgt caaccgggac acgcgagcca ggacgcgacc gcaggatacg
     4921 gccatggaga ataaagaatg aatcgccccc gcctcatccg ccttgccatg gtcgcgctga
     4981 tcccggccgc gatttcgctg ggtatgggca gcgcgcaggc gcaatcgccg cctgcggcga
     5041 cgccgccacc tgccgcaacg cccgccccgg cgccgaccgc aaccccggct ccgcctccgg
     5101 cagccagccc atcggccgct ccgcccgcag ccgcggaaac cccggcgccg gttcagaccg
     5161 cagatccgtt cggcgaggaa atcatcctga cgcagaagcc cgtcgtcatt atcaagggca
     5221 acgccaactg ggattcggcg ttcgacacct tgatcgaatc gttcaagtcg ctgtcagcct
     5281 tgctcgacaa gcagggcatc aagccatcgg gcaattcgat gatcgtctat acctccaccg
     5341 atgacaccgg attcacctac ctcgcggaaa tgccggtcga tcaggatccg aagaatctga
     5401 ccaaggacat gagcatcggc aaatcgcccg agggcaaggc gctgaagttt gtccatcgcg
     5461 gttcctacga caatatggac aatacctatg aggccatcac caatcacctc gacgacaaga
     5521 aactcgaagc caaggatacc ttcatcgagg aatacatcac cgaccccttg aaaaccgcgg
     5581 aggacaagct ggtgatcaac gtctatgtgc cgctgaagta aggctcatga agcatctcac
     5641 tgttctcgcg gttgccatcg ccgccggcac gctggtcgca gcgcccgcac tggcgcagga
     5701 cattccgccg gcagcgattt cggtcactgg cgaagccacg gtatcggtgc cgccggatct
     5761 ggcagtggtc gatggcggcg tctcttcgga ggccaagacc gcgcgggagg cgtcggaagc
     5821 caacagcgcg gccatggcca aggtgctggc cgcgctgaag ggcgccggca tcgacgcaaa
     5881 ggatatccag acctcgcggc tgtcgttgca gccgcaaagc gccccgaacc gcaccggtcc
     5941 gtcggccgtt gtcggctacc gcgccagcaa tcgcgtcacg atccgcgtgc gcgacgttgc
     6001 caaggtcgcc aacgtgatcg acacgctggt gggcgcgggc gccaacgaaa tcggcggcat
     6061 caacttcatg gtgtcgcagg cttcaaagct gctcgacgag gcccgcgaac aggcggtggc
     6121 cgatgcccgc cgcaaggccg agatctatgc caaggccgcc ggcgtcacgc tcggcgcgcc
     6181 gctcagcatc tcggaggaag gctcgcccgg cccgatgcca ttccgcaaga tggcggccgg
     6241 catggcggtt tcggcaccgg tggcacaggg cgaagagacg cttcaagtga cggtgagtgt
     6301 gtcgtgggcg atcaagcaga cacaatgaga tgtcaagatg tcgtcccggc gaacgccggg
     6361 acccagacgc cgtgccgttt cgttttgggc aatggagcaa acaccttctg caacaagcga
     6421 ggtcggtggt tatgggtccc ggccttcgcc ggaacgacga ctatatctcc accacctgcc
     6481 ccggcttgag caccgcaaac cgctcgcgcg ggatcctggc ttcctcgagc gccgcctgca
     6541 atgcgatgac cggcgcatcg atcgcctcat cggtcagttg aaaggtgccg tggtgatgcg
     6601 ccagcgcctg cgccgcgccg caatcggcca gcgcctttac ggcatcgaac gggttcatgt
     6661 gctggtcgcg catgaaccag cgcggctcat aggcgccgat cgggaggatc gccagtcgca
     6721 acgggccgtg cgcctcggcg acgcggcgaa agtgtttacc ctcgccatag ccggaatcgc
     6781 agacgatgta gatcttgccc gctggcgtct gcaggacaaa gctcgcccac agcgccttgt
     6841 tgcggtcgaa caatccgcgc gccgtccagt gccgggtcgg caccagcgtc accgcgatgc
     6901 cgcttccaag ttcaacccgg ttatgccagt cgaacgcttc ggccttgatg gcactgtcgg
     6961 cgctgcgcat ggtgacatcg ttgccgagcg gcgtgatcac gcgaggtgaa aagcttgcag
     7021 cgagcttcga cagcgtcgcg aggtcgagat gatcgtaatg tccatgcgac accagcacga
     7081 cgtcgatcgg aggcagcgcg tcggacgcaa tgccgggatc gttgtgacgc ttcggcccgg
     7141 caaagctcag cggcgaggcg cgtgtcgacc acaccggatc gatcagaatg ttcagcccgg
     7201 aagtctggat cagccagctg gcgtgaccga caaacgatag tctcaccttt tcgccggtaa
     7261 cgcgcggcgg cggggtgtcg gcatgggggc tcggcgccca ttccggccag gcctgccgct
     7321 cccggccggg gccgaactgc cagcgcaaga cttcgccgag cgatttcggc ggcgagccat
     7381 cgggatcgaa gaaatgcgtg ccgtcgaaat gatcg
//
LOCUS       DASWGM010000520         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_174564,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000520 DASWGM010000000
VERSION     DASWGM010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174564"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1944
                     /gene="flhA"
                     /locus_tag="VGA15_15130"
     CDS             <1..1944
                     /gene="flhA"
                     /locus_tag="VGA15_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX9589066.1"
                     /translation="LSILILMTSLFIQAPLEFSWFPTILLISTMLRLSLNLASTRLIL
                     SRGHEGTAAAGHVIEAFGNFVMGGNFVIGIIVFAILVIVNFVVITKGSGRIAEVAARF
                     HLDAMPGKQMAIDADLSAGLIDEKVAKERRKALEDESGFFGAMDGASKFVRGDAVAGL
                     LVVFINVIGGIIIGVAQQGLGFGEAARTYTLLTVGDGLVTQVPALIVSTAAGLLVSKA
                     GVSGAADKALMKQLSGYPQALGMSAGVMLVLAMLPGIPMIPFFLLGSGAAALAFSARK
                     RNRATSAAEAVAAAAPAAAAAAASAAEEPIATALKIDDLKIELGYALLPLVNGPDGTD
                     RLTEQIKALRRSLAVEMGFVMPAVRILDNVQLEANTYIIKIKEVDAGTGKIWPSQFMV
                     MDPAGNQVGVPGIHTIEPTFGLPATWVDASLKEEATLKGYTVVDAATVLSTHLTELLK
                     NNMSDLLSYGEVQKLLKDLPKEQGELVKDIVPSMVTISGIQRVLQLLLAERISIRDLS
                     TILEGIADALAFSRNPAAVVEHVRARLARQICAQNTSMNGYLPLIALSAKWEQAFAES
                     LVGQGEERSLAMQPSKLSEFMTAVRDRFEQAAREGESPVLVTSAAIRPFVRSLVERFR
                     SQTTVLSQAEIHPRARLKTVGSV"
     gene            2391..2591
                     /locus_tag="VGA15_15135"
     CDS             2391..2591
                     /locus_tag="VGA15_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589067.1"
                     /translation="MNHSIYSADRTTHLKIVVVALVAGIAVAGFGVSARNNSDDGYTQ
                     TARVIKAGKAVTVTNSSTSVIR"
BASE COUNT          538 a          881 c          905 g          587 t
ORIGIN      
        1 ctgtcgatcc tgatcctgat gacgtcgttg ttcatccagg cgccactgga attctcgtgg
       61 ttcccgacca tccttctgat ttcgacgatg ctgcggctgt cgctcaatct cgcttccacg
      121 cggctgatcc tgtcgcgcgg acatgagggt accgccgccg ccggccacgt catcgaagcc
      181 ttcggcaatt tcgtgatggg cggcaatttc gtgatcggaa ttatcgtctt cgcgatcctg
      241 gtgatcgtga atttcgtcgt catcaccaag ggttcggggc gcatcgcgga agtcgccgcc
      301 cgctttcacc tcgacgccat gccgggcaag cagatggcga tcgacgccga cctctccgcc
      361 ggcttgatcg acgagaaggt cgctaaagag cgccgcaagg cgctggaaga cgaaagcggc
      421 ttcttcggtg cgatggacgg tgcgtcgaaa ttcgtgcgcg gcgacgcggt cgcgggtctg
      481 ctcgtcgtgt tcatcaacgt catcggcggc atcattatcg gcgtcgccca gcagggcctc
      541 gggttcggcg aagccgcgcg cacctacaca ctgctgaccg tcggcgacgg tctcgtcacc
      601 caggtgccgg cgctgatcgt ctccaccgcg gcgggcttgc tggtctcgaa agcgggcgtc
      661 agcggcgccg ccgacaaggc gctgatgaag cagctgtccg gttatccgca ggcgctcggc
      721 atgtcggctg gagtaatgct ggtgctggca atgctgccgg gcattccgat gatccctttt
      781 ttcctgctgg gcagcggtgc cgcggcgctg gcgttttcgg cgcgcaaacg caatcgcgcc
      841 acctcggcgg ctgaagccgt tgcggcggcc gctccggctg cggccgcggc tgctgcgagc
      901 gcggccgaag agccgattgc gacggcgctg aagatcgacg atctcaagat cgaactcggc
      961 tatgcgctgc tgccgctggt gaatggtccc gacggtaccg accgcctgac cgagcagatc
     1021 aaggcgctgc gccgttcgct ggcggtcgag atgggctttg tgatgccggc ggtgcggatt
     1081 ctcgacaatg tgcagctgga agccaacacc tacatcatca agatcaagga agtggatgcc
     1141 ggcaccggca agatctggcc aagccagttc atggtcatgg accccgccgg aaatcaggtc
     1201 ggagtgcccg gcattcacac catcgagccg accttcgggc tgccggcgac ctgggtcgac
     1261 gccagcctga aggaggaggc cacattgaag ggctataccg tggtcgacgc ggcgaccgtg
     1321 ctctcgaccc acctcaccga gttgctcaag aacaacatgt cggacctttt gtcctatggc
     1381 gaggtgcaaa aactcctgaa ggatctgccg aaggagcaag gcgaactggt caaggacatc
     1441 gtgccgagca tggtcacgat atcgggcatc cagcgcgtgc tgcagctgtt gctcgccgag
     1501 cgtatctcga tccgcgatct ctccaccatt ctcgaaggca tcgccgacgc gctggcgttc
     1561 tcgcgcaacc cggcggcggt ggtcgaacat gttcgcgcgc ggctggcgcg acagatttgc
     1621 gcgcagaaca cctcgatgaa cggctacctg ccgttgatcg cgctgtcggc aaagtgggag
     1681 caggcttttg ccgagtcgct ggtcggccag ggcgaggaac gcagccttgc gatgcagccg
     1741 tccaagctgt ccgagttcat gaccgcggtg cgtgaccgct tcgaacaggc cgcccgcgaa
     1801 ggcgagtcgc cggtattggt cacctcggcg gcgatccgcc ccttcgtgcg ctcgctggtg
     1861 gagcggttca ggtcgcaaac cacggttctg tcgcaggccg aaattcatcc ccgcgcgcgg
     1921 ctgaagacgg tcggaagcgt ctaaagtaga gccttggttc tgattgcatc agaaccaagg
     1981 gctctcatct tttgttttga tgcattttcc tgacgcgaaa cgatatctac tccagatcac
     2041 gtccggggca gggctttcgc ttgaaaacgc ttttacccgg ggcaaatcaa cgcaccgatg
     2101 acggggcttt tgggccacag gcaaatgatt tatcagcccg ggccgattgg caccattttg
     2161 cggcgcaaga tgggcgaaaa tagtaactta ttgtaataat tacattattt ttgatttttg
     2221 aaatcctgac ggcagcgcca gtcgcgggaa ttcaagtctt atcactgcct tgtgatcgcc
     2281 tctttggaac taaagctcga ttccctatgt ttgcaggcat gagatgttga acctgccggt
     2341 ggacgaagtc gaccaatccg caagaggcag taagacggca ggaggcttcg atgaaccatt
     2401 cgatctacag cgcagatcgc acgacgcatc tcaagattgt ggtcgtcgcg ctcgtcgctg
     2461 gtatcgcggt ggcgggcttc ggcgtctcgg ctcgcaacaa ttcggacgac ggatataccc
     2521 agaccgcgcg cgtcatcaag gccggcaagg ccgtgacggt cacgaactcc agtacatccg
     2581 tgatccgcta atgagttttg tgaattcacg cgacccttta gcagcccccc aaagtcgtca
     2641 cgtggatatg tagaagaccc aactacccca agtcgactac cgaaaacgcc cgctccccac
     2701 gggcgttttc tctttgtgga ctgctctctt gttcatgagg cgcaacggct cggcttcgcc
     2761 ctgcggcgag gtggcgtcgc cgcatgcggc acgggcctct aacgggctgg agaaggcgcc
     2821 ggagccgccg gcgggacggc ctcgatcagc ttgccggtaa agaccggcgc cgagtgcgga
     2881 ttgccgtcag gcgatccccc ggcctgctcg a
//
LOCUS       DASWGM010000521         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2136921,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000521 DASWGM010000000
VERSION     DASWGM010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2136921"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VGA15_15140"
     CDS             <1..520
                     /locus_tag="VGA15_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9589068.1"
                     /translation="FFFDFVYPGIGPRMAEPDRLNNIWYQSFHQLEMAPALVGATRES
                     CRTYIGYFLKAWAHRKDAFDDVLETFTDNFLKPGNLAGGFAHYRASHEGRIRMMKGEA
                     PQLAPIDVPACIRWAEHDPLFPYAWTDRLGETFSDLDLARFPAVGHFPHREDPDRTAA
                     EIAAFFTRIGYS"
     gene            complement(753..1877)
                     /locus_tag="VGA15_15145"
     CDS             complement(753..1877)
                     /locus_tag="VGA15_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX9589069.1"
                     /translation="MAEDIGTTTGIARHGATRLPSVDVDSFNIELKDDDGFLGDRASK
                     GAFREILDTLRKPLRKNGDDPLGTKSAEEIGKNGLDEALVGDDVHASALVHGAIEAFA
                     QELAYVTRRFLKTKAWADTERIVVGGGFRQSRVGELAIARTDIILKAEDFEVDLIPIR
                     YHPDEAGLIGCLHLAPSWIFEAHDSILGVDIGGTNIRCGVVETRWKKAPDLSKASVWQ
                     SELWRHADDEPTREGAVKRLVKMLKGLIAAADTQGLKLAPFIGIACPGVINEDGSIEK
                     GAQNLPGNWESSKFNLSASLVEAIPAMGDHDTAVLMHNDGVAQGLSEVPFMQDVERWG
                     VLTIGTGLGNARFTNRRKDKGKSGNDDKDKDEKKDKKSKD"
BASE COUNT          393 a          741 c          666 g          490 t
ORIGIN      
        1 gtttttcttt gacttcgtct atcccggcat cgggccgcgc atggcggagc ccgatcggct
       61 caacaatatc tggtaccagt ccttccatca gctggagatg gcgccagccc tggtcggcgc
      121 gacgcgcgaa agctgccgga cctatatcgg atactttctc aaagcgtggg cgcaccgcaa
      181 ggatgcgttc gacgacgtgc tcgaaacctt cacggataat tttctaaagc ccggcaacct
      241 cgccggcggt tttgcgcatt accgcgccag ccatgagggt cggatccgga tgatgaaggg
      301 tgaggcgccg cagctcgccc cgatcgatgt gccggcctgc atccgctggg ccgagcatga
      361 cccgttgttt ccctacgcct ggaccgaccg gcttggcgag accttctccg atctcgatct
      421 cgcgcgcttt ccagcagtgg gacacttccc gcatcgcgag gaccccgatc gcaccgcagc
      481 cgagatcgcg gcgtttttca cgcgcatcgg ctacagctga tcggcttcat gctgaagaaa
      541 gggtgggacc gccagacgcg gcaaaaaccg gcggtcccgt gtcgcgtcat tgaaattgtg
      601 gccgggaagt gcggcttcgc cgaccagcct gagcgacgat atgaaggtag cgatccgcag
      661 gcgcgcggca acgcgatctc ggttttaacc taactagtca aggttactcg catcgctatt
      721 cgcaaggttg cccgtaatat cagcggcccg cctcaatcct tgctcttctt gtctttcttc
      781 tcgtccttgt ccttgtcgtc attgccgctc ttgcccttgt ccttcctgcg gttggtgaag
      841 cgcgcgttgc cgaggccggt gccgatggtc agcacgcccc agcgctcgac gtcctgcatg
      901 aacggcacct cggacaggcc ctgcgccacg ccgtcattgt gcatcagaac cgcggtgtcg
      961 tgatcgccca tcgccgggat cgcctcgacc aggcttgccg acagattgaa cttgctgctc
     1021 tcccagttgc ccggcaggtt ttgggcgccc ttctcgatcg aaccgtcctc gttgatcacg
     1081 ccggggcatg caatcccgat gaatggtgcg agttttaagc cttgggtgtc ggctgcggcg
     1141 atcaagcctt tcaacatttt caccagtcgt ttgacggcac cctcccgcgt gggctcgtca
     1201 tcggcatgcc gccaaagctc ggattgccag accgacgcct tcgacaaatc cggcgccttt
     1261 ttccagcggg tctcgaccac accgcagcgg atgttggtgc cgccgatatc gacgccaaga
     1321 atgctgtcat gggcttcgaa aatccaggac ggcgccagat gcaggcagcc gatcagcccg
     1381 gcttcatcgg ggtgatagcg gatcgggatc aggtcgacct cgaaatcctc ggccttgaga
     1441 atgatatcgg tgcgcgcgat cgcgagttcg ccgacccggc tctgccgaaa gccgccaccg
     1501 accacgatgc gttcggtatc ggcccaggcc ttggtcttga ggaagcgccg cgtcacatag
     1561 gcgagttcct gtgcgaatgc ctcgatcgcg ccatgcacca gcgcggaggc gtggacgtcg
     1621 tcgcccacca gcgcctcgtc gagcccgttc ttgccgattt cctcggcgga tttggtccct
     1681 aagggatcgt cgccgttctt cctcaacggc ttgcgcagcg tgtccagaat ctcgcggaac
     1741 gcgcccttgc tggcgcggtc gccgagaaag ccgtcatcgt ccttcagctc gatattgaag
     1801 ctgtcgacgt ccaccgaggg cagacgggtc gccccgtgcc gggcgatgcc cgttgtcgta
     1861 ccgatgtctt cagccatttt gcccctgccc tgccgtaatt tgcgggggac aacaagcggg
     1921 gaacccgttg gtttcatggg tcggcgcgta gcgaatagcg agtggcgaat agcgcccctc
     1981 cctcctcact attcgccact cgctattcgc cctttacccc tgaatccctc agttttggcc
     2041 tgttttcgag ccaattcgcc ttgactcgcg gctcgcagcg gctataagtc ccgccacccg
     2101 gcgcgggatt tttctcgcgc tgtttgtttt tgcgtggatc ccaggggtcg ccccccgatc
     2161 gcgtgaaaat tgtatccata acgactgaag aaaaagccgg cccggacctg tccgaggccg
     2221 ggatcgaaca cgaaggaatt caacgatgtt cgcagtcatc aaaaccggcg gccggcaata
     2281 ccgcgtcgtt
//
LOCUS       DASWGM010000522         4106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_315719,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000522 DASWGM010000000
VERSION     DASWGM010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315719"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VGA15_15150"
     CDS             <1..346
                     /locus_tag="VGA15_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GrlR family regulatory protein"
                     /protein_id="HEX9589070.1"
                     /translation="GIKNGLYSIHIRMLDGIDGGNTGVMVLHDGRIHGGDAFFDYVGS
                     YSSADGRWKGELVNHEHTPNQGERPVFGGYEVGIGFSGTYTDEGAVAEATALAGKRSI
                     RFSAVLRKMAEA"
     gene            419..565
                     /locus_tag="VGA15_15155"
     CDS             419..565
                     /locus_tag="VGA15_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589071.1"
                     /translation="MTRAMGREGFLCKPIELSSLGIVLNGGVELGGIKNLEPRTKPCQ
                     LSGA"
     gene            complement(666..1346)
                     /locus_tag="VGA15_15160"
     CDS             complement(666..1346)
                     /locus_tag="VGA15_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589072.1"
                     /translation="MHGKVIVVTGASGALGKVVAEVALARGARVAGVDYGTSQVPATP
                     DRIEFGGVDLSDATQAKKAIDAVAVHFGKIDALINIAGGFAFETVAEGDPKTWQRMYA
                     LNLLTALNASRSAIPHLTASPAARIVNVGAMGALQAGSGMGAYAASKAGVHRLTEALA
                     AEWKGKITVNAVLPSTIDTPANRASMPKADFGKWVTPQELADVILFLASDAASAVTGA
                     LLPVSGRV"
     gene            1675..1812
                     /locus_tag="VGA15_15165"
     CDS             1675..1812
                     /locus_tag="VGA15_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589073.1"
                     /translation="MIVALQNRNGPVSGWELMQIAAVQQAISAFEDVIADLDAELEAA
                     A"
     gene            complement(1998..2744)
                     /locus_tag="VGA15_15170"
     CDS             complement(1998..2744)
                     /locus_tag="VGA15_15170"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX9589074.1"
                     /translation="MPDSPARHLNLAGASNFRDLGGYPAKDGRSVRWRQIFRSNHLGH
                     LTEADIGVLRGLGLKSAFDFRGTEERMAAMCCFEEIAVHSLPIEPTVVAALRTRRASG
                     TALSSGDALDVMRDSYRSYVRQNTTSFRALFAHLLEDRAPLVIHCTAGKDRTGFACAL
                     ILHVLGVPDELIAEDYLLTNHFYRRDPTASSDLPDEVRQVLARVEASFLAAAFDAIRD
                     DYGDLENYLGDGLGLGDGERARLQARYLDD"
     gene            2835..3155
                     /locus_tag="VGA15_15175"
     CDS             2835..3155
                     /locus_tag="VGA15_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEX9589075.1"
                     /translation="MNWCKGIIIKTIVFGAALAGMASGASPALAQTFQNYRCADGSRF
                     IVGFFQYDKRAHLQIDGRAVTLAKRLALSGSRYSGDGVTLKIGRAGTTIRHAKRPTTA
                     CELI"
     gene            complement(3251..3505)
                     /locus_tag="VGA15_15180"
     CDS             complement(3251..3505)
                     /locus_tag="VGA15_15180"
                     /inference="COORDINATES: protein motif:HMM:NF022384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2735 domain-containing protein"
                     /protein_id="HEX9589076.1"
                     /translation="MKNESGAPLSQSASTQGEMMMNTSLNQGSARIYQFPAGGRSGVS
                     ARRQGETQAPAELASPRVSEVGGAWYHDAAIQESKPEREH"
     gene            4001..>4106
                     /locus_tag="VGA15_15185"
     CDS             4001..>4106
                     /locus_tag="VGA15_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX9589077.1"
                     /translation="MTKYKLEYIWLDGYTPTPNLRGKTQIKEFDSFPAL"
BASE COUNT          784 a         1231 c         1310 g          781 t
ORIGIN      
        1 cggcatcaaa aacggcctct actcgattca catccggatg ctcgacggca tcgacggcgg
       61 caacaccggc gtcatggtgc tgcacgatgg ccggatccac ggcggcgatg ccttcttcga
      121 ctatgtcggc tcctactcca gcgcagacgg taggtggaag ggcgaactcg tcaaccacga
      181 gcacacgccg aaccagggcg agcggccggt gttcggcggc tacgaggtcg gcatcggctt
      241 ttccggcacc tataccgacg agggcgcggt cgcggaagca accgcgctgg ccggcaagcg
      301 cagcattcgc ttcagcgcgg tgctgaggaa gatggcggag gcgtaagcgt atcttagccg
      361 atcaatcgta atcggcgtgc gagccgaccc gagttggagt gcctcagaag tcccgggatt
      421 gacccgcgcg atgggccgcg agggcttcct ctgcaaaccc atcgagcttt ccagtctcgg
      481 catcgtgctc aatggcggcg tcgaactggg cggcatcaaa aacctcgaac cacgcacgaa
      541 gccgtgccag ctctcggggg cctaattgct cgatagccct ttcaatgtct tcggcggtcg
      601 tcatgcgaga gatatagcac ccgtccggct tcgcgaaagg gtgcgtgaga atgcggagag
      661 gacgcctaca cccgcccgct caccggcagc agcgccccgg tgacggcgct ggcggcgtcg
      721 cttgcgagaa acaggatcac gtcggcgagt tcttgcggcg tcacccattt tccgaaatca
      781 gccttcggca tgctggcgcg gttggccggg gtgtcgatgg tcgagggcag cacggcattg
      841 acggtgatct tgcctttcca ctcggcggcg agcgcctcgg tgaggcgatg cacgccggcc
      901 ttggaggcgg cgtaggcgcc catgccggag cccgcctgca gcgcgcccat cgcgccgaca
      961 ttgacgatcc gtgcggcggg cgaagcagtg agatgcggga tcgccgaccg cgacgcattc
     1021 agcgcggtga gcaggttgag cgcatacatg cgctgccagg ttttcggatc gccctcggcg
     1081 accgtctcga atgcaaagcc gccggcgata ttgatcagcg cgtcgatctt gccgaaatgc
     1141 accgcgaccg catcgatggc ttttttggct tgcgtcgcat ccgacagatc gacgccgccg
     1201 aactcgatcc ggtccggcgt cgccgggacc tgcgatgtcc cgtaatccac cccggccacc
     1261 cgggcgcccc gcgccagcgc cacttccgca accactttgc caagcgcgcc cgaggcgccg
     1321 gtcacgacaa tgacttttcc gtgcatgttt ggtctccggc gccagggggt aagcagggtc
     1381 cacactaccg ccaagcgaaa ttcatttgca atgttgcgaa attttgtaag cgagatgggc
     1441 gctccaaggg ttcgctcctg cggcgcgatt gcgggcgcac cgccgccaac ggccctgggg
     1501 ccccctaggt cttccgcgta ctaatcggta aaatttgccg gagttttgta agtggaaagc
     1561 agaagcatcg cgctaggctt catgaaagcc aatcaatagg cagggtgcgt tacgatgaga
     1621 cagctagccg atcacggttt accacttgtt cagcttaagg agcagcggcg ggatttgatc
     1681 gtcgcgctgc aaaatcgcaa cggcccggtc agcggctggg aactgatgca gattgcagcg
     1741 gttcagcagg cgatttcggc attcgaggac gtcatcgccg acctcgacgc tgagctggaa
     1801 gcagccgcgt aaggtcgttt cctggcggat cggccgggtt cgacccggct atggctggcc
     1861 atacgtcaac gaaccggcaa ccggcatggc cgcgaggccg atgccgcccg gctttggccg
     1921 aagcaacgcc ttcgttgcat acacctggcg cccgaaccat gaaaggatgg gacaccggca
     1981 gcgctgcacg tggcgtgtca gtcatccagg tagcgcgctt gcagccttgc gcgttcgccg
     2041 tcgccgaggc ccaacccgtc gccgaggtag ttttcgagat cgccgtagtc gtcgcggatg
     2101 gcatcgaatg ccgcggcgag gaacgatgct tcgaccctcg ccagcacctg ccggacctcg
     2161 tcgggaagat cggaactggc ggtggggtcg cggcggtaga agtggttggt gagcagatag
     2221 tcttccgcga tcagttcgtc cggcacgccc agcacatgca ggatcagcgc gcacgcgaac
     2281 ccggtgcgat ccttgcccgc ggtgcagtga atgaccagcg gcgcgcggtc ctcaagcagg
     2341 tgagcgaaca gcgcgcggaa gcttgttgtg ttttggcgca catagctgcg gtaggaatcg
     2401 cgcatgacat ccagcgcgtc gccggacgac agggcggtac cgctggcgcg ccgcgtgcgc
     2461 agcgccgcga ccacggtcgg ctcgatcggc agcgaatgga ctgcgatctc ctcaaaacag
     2521 cacatcgccg ccatgcgttc ctcggtgccg cgaaaatcga aagcgctttt caggccgagg
     2581 ccgcgcagca ccccaatgtc ggcttcagtg agatggccga gatggttgga gcggaagatc
     2641 tggcgccagc ggacgctgcg gccgtccttg gcgggatagc cgccgaggtc gcggaaattg
     2701 ctggcgccgg caagattgag gtgacgggca ggagaatctg gcatgggctt tagcttaggc
     2761 gatcctctgc ggcgaacaaa tgacggggag ggcgtatcgc tctggactaa ctgggctcat
     2821 ttgggggggc gaacatgaat tggtgcaagg gcattattat caagactatt gttttcggcg
     2881 cggcgttggc gggaatggcg agcggcgcat cgcccgcgtt ggcgcagaca ttccagaact
     2941 atcgctgtgc cgacggatcg cggttcattg tcggattttt ccaatatgac aagcgcgcgc
     3001 atctgcagat cgatggaagg gccgtgacgc tggccaagcg cctggctttg tccggctcgc
     3061 gttattcggg cgatggcgtc acgttgaaga tcggcagggc aggtaccacg atcaggcatg
     3121 ccaaacgacc gacgacggcg tgcgagctga tttgaaaaaa gggccgaaac gtttccgttt
     3181 cgaccctttt accgctgttg aaatcgcacc gaagaccagc gatgcattgg catcgaatgc
     3241 gctgccggca tcagtgctca cgctccggtt tggactcctg gattgccgca tcgtgatacc
     3301 aggctccgcc aacttcgctc acgcgcggcg aagcaagctc ggccggcgcc tgtgtctcgc
     3361 cctggcgacg tgcgctgacg cccgagcggc ctcctgcagg gaattggtag atccttgcgg
     3421 acccctgatt caaactcgta ttcatcatca tctctccttg cgtcgatgcg ctttgactca
     3481 atggtgcgcc cgactcgttc ttcattggtt actgaaaccg atatcgtccg tgcactccgc
     3541 cagtgcaaca tgaccaaaac aaaatcgttt cctgtataaa atgtaggcat tcctgattct
     3601 gcaccctgca aagcacccgt tgggtggcta acgcttgagc catcgccaag gttgcggagc
     3661 gaggtggatt cccggacaaa tgttgccggc gatttggcgc gggtttaatc gcagccatca
     3721 gacggccgcc gactgctgcc taatcaggcg tagtctgggc ctaaatcagg gccatcaggc
     3781 cttcgtccga atcgctaccc gcgcggcttt gccgcacccc taaaatgcga ccgccaggcg
     3841 ggagaagcgg ggtatttcgc cggctggccg gggtaccgca gtgcagcgat ttggcacctt
     3901 aattgcttgg taagtactgg ccggtggtgg ccgggtagac gcgtggtgac cataggcttc
     3961 ggtttccacc tttcgcatca tcaactcatg agagagctca atgacaaagt ataagctcga
     4021 gtatatctgg ctcgacggat atacgccgac gccgaatttg cgcggcaaaa ctcagatcaa
     4081 ggaattcgat tcatttccgg cgctcg
//
LOCUS       DASWGM010000523         5110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_174809,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000523 DASWGM010000000
VERSION     DASWGM010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174809"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VGA15_15190"
     CDS             complement(<1..1023)
                     /locus_tag="VGA15_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX9589078.1"
                     /translation="MLHTELIAPVSELLERHRQERPDKVAYWDAARSVTYSQLASRTA
                     SIAAGFSSNGVREGDRIAIYLPNGVDWIEACFAGLRAGAVIVPISYDAAEAEIAYRLS
                     DADCGTVITTPARKELASRLAAGLNGPVSLLFAGAGAGEAGPCLDELARANADLAPDP
                     DDIDRPSFIIYTSGTTGRAKGVLLSVRGMMWIVAACWAPICKLNDKDVVLSPLPLFHS
                     YALNLSVLGVLAVGASEHILERFSPDKVLELLQSGRYSVFPGVPTMFHYLLQRAQESG
                     VHRLGDTRLCISAGAIMPAALNRSFEERFGTTLVDSYGITETSTMVTMNWTSGARPMG
                     SCGLPLP"
     gene            complement(1040..1789)
                     /locus_tag="VGA15_15195"
     CDS             complement(1040..1789)
                     /locus_tag="VGA15_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589079.1"
                     /translation="MNDLKGRVALVTGGGRDVGAGISQALAEAGASVAVNYHSSRDEA
                     EAVVAQIQKAGGKAKPYQADIADLEAVRSMVASVKSDFGGLDILVNNAGLVLRKRFND
                     TTPEDWHKQIDTCLYGAIHCCHEAAPLLEASGRGRIISIMGDSSRVGESGLAIAAAAR
                     AGTIALMKSLAREMGRTGTTANSIALGLIESAHDRTWVDANYDKLVKAYPIRRLGQPS
                     DVAPMVALLASDAGSWITGQVISISGGFSMV"
     gene            complement(1786..4185)
                     /locus_tag="VGA15_15200"
     CDS             complement(1786..4185)
                     /locus_tag="VGA15_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9589080.1"
                     /translation="MSKDDVVQTSDLLRPRHVGMPIRRTEDPRLLTGNGEYTADRKPD
                     RALHVAFRRSEQPHATILRIDATQALTAPGVVAVFTADDLAEDYKPVIPFSRMPNYYA
                     TPITALASKKVRYVGEAVVAVIAEDRYLAEDALELIEIEYAPLGAVTDPEFAASEDAP
                     LLHEEAATNVLIAREFGKGDAAADLAAAHVRVRGRFEMTRKAGLAIEPRSYCAEFEGR
                     RDSITLYTSSNIPGIVRDALAESLDLPGSRLRVVAPDVGGSFGSKGSLYPEEILLCVA
                     ARKLRRSLKWTADRLEDISSSSQAFAEIVDAEMGFDQNGIAIALEADVIGDVGAYSIY
                     PWTCGLEPVQVVSFLPGPYKIRSYRGAVRGVATNKPPTGPYRGVGRPISTFVAERLMD
                     MGAKALGIDPLEIRRRNLVREDEFPFRIASGIIWDKAGFVECLEAAVQAGEYEKLRGQ
                     QRAARAEGRYFGIGLATYAELTGIGSRIAVAPGMPINTGSETAKIRIDSTGAITAAFG
                     VASHGQGLETTLAQIIADDLGARFDDIRVVQGDSDAVPMSTGTYASRSAVLGGGAAKH
                     ASLILREKIKKVASHLLEAAADDIDVAGGRATVMGTDRAVTFRQIAKAVYSDMRTLPV
                     EARQELEATYTYDPINGTTACATHLAAVEVDPTTCFVKIHKYVVAEDCGRIINPMIVD
                     GQVHGGVAQGIGAALYEEVIYDEDGQLVSASLADYVIPSAVEIPHMDVVHIESCSAVA
                     GGFRGMGEGGTIGAPAAIANAVSDALSALDIQVSILPITPERIFRLLEQARVKAKGSR
                     Q"
     gene            complement(4178..4939)
                     /locus_tag="VGA15_15205"
     CDS             complement(4178..4939)
                     /locus_tag="VGA15_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX9589081.1"
                     /translation="MKLQFLSAGRLRLKKSVYIRGADRSETFEAPVISALIRHKQGNV
                     LFDTGCHPSVVEHGEERWGSMIKVVTPVMRAEDTLLPNLACVGIDPNDIDVVVNSHFH
                     PDHCGCNQFFRKATILAHAREIEAAKAAGAEAAGYLRADWDHGQPMDPVNGERDVFGD
                     GKLVLIPLPGHTPGSLGAAVHLDRDGSFLLASDAVSLRENLDTDTSPRNTWNAEALLN
                     SYGEIRRIEKSGATVICGHDDAQWQSLRKGGEAYE"
     gene            complement(4936..5097)
                     /locus_tag="VGA15_15210"
     CDS             complement(4936..5097)
                     /locus_tag="VGA15_15210"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9589082.1"
                     /translation="MLMTAMDLLKKYPLASDEEIREGLSGNLCRCTGYQNIVAAIRSA
                     VSLRNGSAS"
BASE COUNT          960 a         1661 c         1510 g          979 t
ORIGIN      
        1 cggcagcgga aggccgcacg accccatcgg gcgagctcca ctggtccagt tcatggtgac
       61 catcgtcgag gtttcggtga tgccataact gtcgaccagc gttgtcccga aacgctcctc
      121 gaatgaccgg ttcaatgcag ccggcatgat cgcgccggcg gaaatgcata atcgcgtgtc
      181 tccgagccga tggacaccgg attcctgggc acgctgcaac agatagtgaa acatcgtcgg
      241 cacgcccgga aaaaccgaat atcttccgga ctgcaacagc tccaggactt tatccggaga
      301 gaaccgctcc aggatatgct cgctggctcc gaccgccagc acgccgagca ccgaaagatt
      361 cagcgcatag gaatgaaaca gaggaagcgg cgacagcaca acgtccttgt cattcaactt
      421 gcagatcggc gcccagcacg ctgcgacaat ccacatcatg ccgcggaccg acaaaaggac
      481 gcccttggcg cggccggttg tgccggacgt atagatgatg aacgacggac gatcgatgtc
      541 atcaggatcc ggggcgaggt ccgcgttggc ccgcgccaat tcgtccaggc acggaccggc
      601 ttccccggcg ccggcgcctg cgaacagcag tgaaaccggg ccattcaacc ccgccgccag
      661 tctgctggcg agctccttac gggccggtgt cgttatcacc gtgccgcaat cggcatcgga
      721 tagtcggtag gcgatctccg cttcggcggc gtcatagctg atcggcacga tgaccgcgcc
      781 ggcacgcaag ccggcgaagc aggcttcgat ccagtctacc ccgttcggaa gatagatggc
      841 gatacgatcg ccttcccgca ccccgttgct tgaaaagccg gcggcgatcg acgccgtacg
      901 gcttgcgagc tgcgaatagg tgaccgatct cgcggcgtcc cagtaagcga ccttgtcggg
      961 ccgctcctgt cgatgccgct cgagcagttc cgataccggt gcgatcagct ccgtatgtaa
     1021 cattgttttc ctccgcccat cagaccatgc tgaaaccgcc gctgatgctg atcacctggc
     1081 ctgtaatcca gctaccggca tcagacgcga gaagggcgac catgggagcc acgtcggacg
     1141 gctggccgag acggcggatc ggatacgcct tgacgagctt atcgtagttc gcatccaccc
     1201 aagttctatc atgtgcggat tcgatcaggc cgagcgcgat cgaattggcc gtcgtgccgg
     1261 ttcgtcccat ttcccgggcc agcgacttca tcagcgcaat ggttccggcg cgcgccgccg
     1321 ctgcgatcgc gagcccggat tcgccgaccc gggacgagtc tcccatgatg gaaataattc
     1381 ggccgcggcc cgacgcctcc agcaagggag cggcctcgtg gcagcagtgg atcgcgccat
     1441 agaggcaggt atcgatctgc ttgtgccaat cctccggcgt ggtgtcgttg aatcgtttgc
     1501 gcagcaccag gccggcattg ttgaccagaa tatccaggcc gccgaaatca gacttgacgc
     1561 tcgccaccat gcttctgacc gcttcgagat ctgcgatatc cgcctggtat ggcttcgcct
     1621 tgccgccggc cttctggatt tgcgcaacga ccgcttcggc ctcatctcgc gagctgtgat
     1681 aattcaccgc gaccgacgcc cccgcctcgg caagcgcctg cgagatcccg gcgccgacat
     1741 ccctgccgcc gccggtaaca agggcgacgc gccctttcag atcgttcatt gtcggcttcc
     1801 tttcgctttg acgcgtgcct gctccagcag cctgaagatc cgctccggag tgatgggtag
     1861 aattgatacc tggatgtcga gcgccgatag cgcatcggaa accgcattgg cgatcgcggc
     1921 gggcgcgccg atcgttcctc cctcgcccat gccgcgaaag ccgccggcaa ccgccgaaca
     1981 gctttcgata tggactacgt ccatatgggg aatctcgaca gcggacggta tgacgtaatc
     2041 ggcaaggctc gcgctcacga gctgaccgtc ttcgtcgtag atcacctctt cgtagagcgc
     2101 agcaccaata ccctgcgcga caccgccgtg aacctgaccg tccacgatca tcggattgat
     2161 gatgcggccg caatcctccg ccaccacata tttgtggatc ttcacaaaac aggtggtggg
     2221 atcgacctcg accgcagcca gatgggtcgc gcatgccgtc gtcccgttga tgggatcgta
     2281 ggtgtaggtc gcctccagtt cctgacgcgc ctcgaccggc aatgtcctca tatccgaata
     2341 caccgccttg gcgatctgcc tgaaggtgac cgcgcgatcg gttcccatga cagtggcgcg
     2401 gccacccgcg acgtcaatat cgtcggcggc ggcttcgagc agatgcgacg cgaccttctt
     2461 gattttctcc cgcaggatca gcgaagcatg tttcgccgcc cctccgccca ggaccgcgct
     2521 gcggctggcg taggttccgg tcgacatcgg cacggcgtcg ctgtcaccct ggaccacccg
     2581 aatatcgtca aatcttgcgc cgagatcgtc ggcgatgatc tgggcgagcg tggtttccaa
     2641 cccctgcccg tgcgaggcga cgccgaacgc cgccgtgatt gcgccggtcg agtcgatgcg
     2701 gattttggcg gtttccgagc cggtgttgat cggcattccc ggcgcaacgg caatccgcga
     2761 gccaatcccc gtcagttcgg cataggtggc gaggccgatg ccgaaatagc gcccctcggc
     2821 ccgtgccgcg cgttgttgac cgcgcagctt ttcgtattcg ccagcctgca cggcggcttc
     2881 gagacattcg acgaagcccg ctttgtccca gatgattccg gaagcgatgc gaaatggaaa
     2941 ttcgtcctcg cgaaccaggt tgcgtcggcg tatctcgagc gggtcgatgc cgagcgcctt
     3001 ggcgcccata tccatcaggc gttccgcgac gaaagtggaa atcggccggc cgaccccgcg
     3061 atagggtccg gtcggtggtt tgttggtcgc aaccccgcgc acggcgcccc tgtaagacct
     3121 gatcttgtac ggccccggca ggaagctgac gacctggacg ggttcgagcc cgcatgtcca
     3181 gggatagatc gagtaggcgc cgacgtcgcc gatcacatcg gcctccagcg cgatggctat
     3241 cccgttctga tcaaagccca tttcggcatc gacgatctcg gcgaatgcct gactggaact
     3301 gctgatatcc tcgagccgat ccgcggtcca tttcagcgaa cggcgcaact ttctcgccgc
     3361 cacgcataac agaatctcct cgggatagag cgatcccttg gaaccaaaac tgccgccgac
     3421 atccggcgcg accacccgca accggctgcc cggcagatcg agcgattccg ccagcgcgtc
     3481 gcgcacgatg ccgggtatgt tggacgaggt gtagagggta atcgaatccc ttctgccttc
     3541 aaactcagcg caatagctgc ggggctcgat ggcgagcccg gcctttcggg tcatttcaaa
     3601 gcgaccgcgg acacggacat gcgcggccgc aagatccgca gccgcgtccc ccttcccgaa
     3661 ctctctcgcg atcagcacat tggttgcggc ttcctcgtga agcagcggcg cgtcctctga
     3721 agccgcgaat tcgggatcgg tcaccgcccc caacggggca tactcgatct cgatcagttc
     3781 cagggcgtcc tcggcgaggt accgatcctc ggcgatgacg gccaccacgg cttccccgac
     3841 atagcgcacc tttttcgagg ccagggccgt aataggcgtc gcgtaatagt tcggcatgcg
     3901 agaaaacggg atgaccggct tgtagtcctc tgccaagtca tcggcggtga aaaccgccac
     3961 gacacccggc gccgtcagcg cttgggtcgc gtcgatgcgc aggatcgtcg cgtgaggttg
     4021 ttcgctgcgg cggaaagcaa cgtggagcgc gcgatcaggc ttgcgatcag cggtatattc
     4081 gccgttgcct gtcagtagcc gcggatcttc ggtccgcctg atcggcatgc cgacatgacg
     4141 cggtcgcagc aggtctgacg tctggacgac gtcatcctta ctcataggcc tctcccccct
     4201 tgcgcaggct ctgccattga gcgtcatcat ggccgcagat gaccgttgcg ccggacttct
     4261 cgatccggcg tatttcgccg taggagttca gcaatgcttc cgcattccag gtattacgcg
     4321 gcgacgtatc ggtatcgagg ttttcgcgca ggctcaccgc atctgacgca agcagaaaag
     4381 acccgtcgcg atcgaggtga acggcggcgc cgagcgaccc cggtgtatgg ccgggcagcg
     4441 ggatcaatac cagtttgccg tcaccgaaca catcccgttc gccgttcacg ggatccatcg
     4501 gctgtccatg gtcccaatcg gcccgcagat aaccggcagc ttccgcgccc gccgctttcg
     4561 cggcctcgat ctccctggca tgcgccagga tggtcgcttt cctgaaaaac tgattgcagc
     4621 cgcaatggtc ggggtggaaa tgcgaattga ccacgacatc aatatcgttc gggtcgatgc
     4681 cgacgcaggc cagatttggc agcagcgtat cctccgcccg catcaccggg gtcacgactt
     4741 taatcatcga gccccatctt tcttcgccat gctcgacgac ggaggggtga cacccggtgt
     4801 cgaacaacac attgccctgc ttgtgccgga tcagcgcgct gatcacgggc gcttcaaacg
     4861 tctcgcttcg atcggcgccc ctgatataga cgctcttctt caagcgaagc cggccggcgg
     4921 aaagaaactg cagcttcatg aagcgctccc gtttcgcaag ctgaccgctg agcggatcgc
     4981 agcgacgata ttctgatagc cggtgcagcg gcaaaggttg ccggacagtc cctcgcggat
     5041 ttcctcatcg ctggccagtg gatatttttt caagagatcc attgccgtca tcagcattcc
     5101 cggcgtgcag
//
LOCUS       DASWGM010000524        17241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_315980,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000524 DASWGM010000000
VERSION     DASWGM010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17241
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315980"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGA15_15215"
     CDS             complement(<1..266)
                     /locus_tag="VGA15_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9589083.1"
                     /translation="MACGLNARADEHAHEDHRPSFWVRWLLSTNHKDIGTLYLAFAIG
                     AGLIGGFISVLMRIELMRPGDHLLHGDTQLYNVIITAHGLIMVF"
     gene            complement(307..1179)
                     /gene="coxB"
                     /locus_tag="VGA15_15220"
     CDS             complement(307..1179)
                     /gene="coxB"
                     /locus_tag="VGA15_15220"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9589084.1"
                     /translation="MGSGWSVDIKRGTLAIGAALTLLAWAVISPAALAEEPVNWQLGM
                     QAAASPVREQIDSLHDKLLVIATVIVLFVLALLVVIIVRFGAKRHPISSPTTGNRLIE
                     VTWTTVPVLILVYIAIPSFKLLYYADRVRTADFTVNVIGHQWYWSYEYPDQGGLTFDS
                     TMLQDDDLATYREPRLLGVDKPMIVPVGATVRVIVGGTDVIHSWFVPPLGVQEYSVIG
                     RKNESWMRIDRPGTYYGQCNQICGMNHPFMPIEVKAVSQGDFNRWIKNPDSLWNVPQK
                     KASLIVVPNGSDAG"
     gene            complement(1290..1673)
                     /locus_tag="VGA15_15225"
     CDS             complement(1290..1673)
                     /locus_tag="VGA15_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX9589085.1"
                     /translation="MKSVVVVLLVTLAWNGAVLAQDVAAGEQSFKKCAACHAVGEGAK
                     NKIGPELNGLDGRKAASVQGYSYSDANKKSGITWDDASFRDYIKDPKAKLPGTKMVFA
                     GIKNDQDVSDLWAYLRQFDSTGKKK"
     gene            complement(2320..2538)
                     /locus_tag="VGA15_15230"
     CDS             complement(2320..2538)
                     /locus_tag="VGA15_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589086.1"
                     /translation="MSDHSIKRLHRIVGEINAWLVVIAIGLATFDLVVPVALEMPAVP
                     GKCLNCDNIARLPDGTVEPAAAVGAFAQ"
     gene            complement(3006..3941)
                     /locus_tag="VGA15_15235"
     CDS             complement(3006..3941)
                     /locus_tag="VGA15_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9589087.1"
                     /translation="MATIKTLARVIATLLIVAAGCAGAYELWNYYMLSPWTRDARVRA
                     DVVTIVPDVAGFVNDIRVKDNQLVHKGDILLILDQERYKRAAATAEASVAARKAEMLM
                     RQGDADRRAKLTNLSISPEAKEDAQHVAASATAAYQQAVADRSTASLNLERTVIRAPV
                     NGFVTNPVVIGQYAAVGTKLMALIDSDSYRVEGYFEETKIPVIKPGARVEIHLMSGEP
                     ALQGHIESISRGITDRDNVNGPELLANVNPTFEWVRLAQRIPVRIHIDEIPESVRISA
                     GMTCTVVVAAPAREWAITSLFRNGSHRQPPAAARP"
     gene            complement(3943..4143)
                     /locus_tag="VGA15_15240"
     CDS             complement(3943..4143)
                     /locus_tag="VGA15_15240"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEX9589088.1"
                     /translation="MLRELALFGALAPSLLLYFFAAILLFVVADRLIARLGVYRLLWH
                     PPLARFGLFLCVFSVLVLATRP"
     gene            complement(4133..6208)
                     /locus_tag="VGA15_15245"
     CDS             complement(4133..6208)
                     /locus_tag="VGA15_15245"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX9589089.1"
                     /translation="MPDTDPARWFFGPRWIPWIFAAKTTAAGLLALLVAFTFNLDQPQ
                     WALLTVFIVSQPKDGLVLAKSFYRIIGTVIGAGGALVLVALFAQERVLFLGALAVWIA
                     FCTFGSQYARNWAAYGFVLSGYTVAIVGIPGALDPGNAFFIAVARVSEISLGIMTAAA
                     VSHLVLPMSLAGALRRAVATGRADLADYAAALLLGGRDTTSLRTKLLGQVVAIENLRA
                     SAVFEDREIQNRSGVLRRLDIAMLGVVDVAHLLGRSLDWLRRGRAVIGPGLDGTLTRA
                     AAAIDVWRGGKLDAAGLSRRLVQASASLPLARELCLAPFAADEDVIRCAAVIGRLREF
                     FAAMVVYAEADQAFAAGEPGSNRRVDFGLANDFASAVWTGLRAALAVLLVSGFWIAAN
                     WPHGPTAVILAAVATARLATMGHAVPLAIAATLIFSLSTIPAFVLVETLLPLASGFAM
                     FALVVAPMLFLCAFMMAHERTMVIGFLSALLFASVGAFQNRMVYDPIGLVNTSIAATL
                     AAAVAMILWAIVAPETPEAAHRRFGRAARKLLARIAAPRPRIGLAEFETAMTEALDRL
                     RGHLRPDRPADLAALDAGTALLITGRELIRVRDDGRPTPAKMDVEDKVVRFLASNKKL
                     TLDRALRAAQDASVTCLAELRDDRVGVAEARTAVREMVAFAAIRDELERGGELLLDER
                     ATGAPAHVA"
     gene            complement(6382..7677)
                     /locus_tag="VGA15_15250"
     CDS             complement(6382..7677)
                     /locus_tag="VGA15_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9589090.1"
                     /translation="MAKPTKTNLPRRPSRPPFDCIALLLQGGGALGAYQAGVYEALAE
                     ANLHPDWTAGISIGAINSALIAGNPPESRVEKLREFWQQITTPLPLLKHASQAAMVAE
                     PTAGPDAWQGTYAAKMWEFWERVGSAAVSSGLHGLELQTVKGDAARGLFNKLSAGRVL
                     MTGAAGFFTPRLPVPWLCPDGSLGATSYYDTGALRTTLERLIDFDRIDAAEMRLSMGA
                     VNVTTGNLVYFDNTSHAIRPEHVMASGALPPGFPAVEIDGERYWDGGLVSNTPLQWVV
                     QSEPQRDTLAFQVDLWSASGEFPRNLPEVTTRQKEIQYSSRTRANSDRFKQIQRLRAA
                     VASLLEKLPEELRSMPEVEILKPASEHKVYNLVELVYRAKQYEGDSKDYEFSLMSMED
                     HWRAGYYDTVRTLQHPEVLKRPASADGVFILDLAQEGRE"
     gene            complement(7710..9662)
                     /locus_tag="VGA15_15255"
     CDS             complement(7710..9662)
                     /locus_tag="VGA15_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9589091.1"
                     /translation="MRNLAGWFVLGTAVLSSNGAYAAGDATAGKTVFENQCASCHSTV
                     PGGEGFGPSLARVIDRKSGAVAGYNYSTAMANAGLTWDAKTLDGFLASTTKKVPGTSM
                     SVEIPSEADRANVIAYLATLGVAAAANAPATSAAPANMPLGEGPTQEELLHAAGDTQN
                     WLYASKDYTGQRFVDLDQINASNAASLRAVCIYRSNNAGATQTNPLVYKGVMYLTIDK
                     QIVAIDAATCRERWTYNWQTKDQVLSATNRGVALKDGRVVRGTADGYLVAVDMDKGTL
                     LWSRKIADAKASQYLSMPPLIFGDLIIYGPAGADWGAKNWIGAFKLDTGEPVWRFNLI
                     PDANEAGADSWTNPQAREHGGGAVWTPLSLDAEKGVLFVPVGNPSPDFNGEVRPGTNL
                     YTDSVVALDAKTGKLLWYQQFRANDMHDWDLSQVSPIFRAAVNGKPRNLLTISGKDGL
                     LRMVDRDSHELLYEMPITTRENPEAVPTVAGVHVCPGLLGGMEWNGPAYNPRTSTLFV
                     ATVDWCGTFKRTEGPPQFAENAHYYGGSVTPDARDQAKGWLYAIDAASGSVHWRQHWP
                     TPLVAGITATSGGVLFTGDLNNDFLAIDAREGNTLYRFNTGGSIGGGVITYALDGKQY
                     VATTSGVVSGFFGGTGTSAVIVFALP"
     gene            complement(9720..9842)
                     /locus_tag="VGA15_15260"
     CDS             complement(9720..9842)
                     /locus_tag="VGA15_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589092.1"
                     /translation="MAVDEELERHQETWQGFTRIVQGGVALVVSVLLLLEFFLL"
     gene            complement(9888..10832)
                     /locus_tag="VGA15_15265"
     CDS             complement(9888..10832)
                     /locus_tag="VGA15_15265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEX9589093.1"
                     /translation="MTRGVTLAAGPFDGLAPYLRQIREFPMLEPEEEYLLAKRWREHG
                     DSGAAQRLVSSHLRLVVKIAKCCRSHTLPLSELISEGNVGLMQAVDRFDPERGFRLTT
                     YAVWWIRAAIQRHIMSSSSLVRMGTTTAQKKLFFGLRSCKEQLQAPDDGELSPENARK
                     IAGWLDVSEADVIEMNHRLAGPDLSLNGSSDRPISEYGEEWLDILPDSSPDQETLVGE
                     RTELRQRLHRLNLALKHLNPRERDIISERRLREKPTTLRELGKRYAVSPERMRQIEAR
                     ALEKLRRSMHAARSPSKRSRRGERPSAGKPNVRAQAHL"
     gene            complement(10854..12062)
                     /gene="hemA"
                     /locus_tag="VGA15_15270"
     CDS             complement(10854..12062)
                     /gene="hemA"
                     /locus_tag="VGA15_15270"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX9589094.1"
                     /translation="MYDYEAFFKARLDTLRTEGRYRLFADLERRAGAFPRAFHHGLGA
                     EVTVWCSNDYLGMGQHPAVLAAMHAAIDSIGAGAGGTRNIAGTGHHHVLLEREIADLH
                     GKEAALIFTSGYVANEAALATLGSQIPGCMIFSDALNHASMIQGIRHGGAEKYRFRHN
                     DVRDLDRLLAAADPTCPKLVCFESVYSMDGDIAPIAELCDVAERHGAMTYLDEVHAVG
                     LYGPRGGGIAEREGLMHRVTAIQGTLAKAIGVIGGYVAASAALVDFLRSHASGFIFTT
                     ALPPAIAAGALASVRHLKADQSVRARHQERAARLKTELARAGLPVMPSQSHIVPVAVG
                     DASLCKAASDELLLHHRIYVQPINYPTVPRGTERLRLTPTPLHTDHDIERLVFALRDV
                     WNWLGLKAAA"
     gene            complement(12321..13535)
                     /locus_tag="VGA15_15275"
     CDS             complement(12321..13535)
                     /locus_tag="VGA15_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9589095.1"
                     /translation="MASAPLDSTSSAQALPEPDGDFYLVAKILSKEDNDLRIKVRTFM
                     EEEVQPIINEFWTTGEFPFDLLPKFRELQIAGLPIRGYGCPGRSTTLMGFVMMELARV
                     DASIATFFGAHTGLAMGSIYLCGSEEQKQRWLPEMAALRKVGSFGLTEPEVGSAVSRG
                     LQTTARLEGDTWVLSGQKKWIGNATWADVTIVWARDVADDQVKGFIVEKGSPGLHVEK
                     IQNKMALRVVQSGLITLQECRVGEANRLKKANSFKDTANVLRLARAAVAWESVGCATG
                     AYEHALRYAQHRSQFGRRIARFQLVQDLLFRMLGNITSMQALCLRLSQLQDQGLMRDE
                     HASLAKGICIVRMRETVGWARELLGANGILLDHHIGRFAADAEALYSYEGTREMNALV
                     VGKAITGFSAFV"
     gene            complement(14135..17014)
                     /locus_tag="VGA15_15280"
     CDS             complement(14135..17014)
                     /locus_tag="VGA15_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX9589096.1"
                     /translation="MRDGALQFAEPAVSIERSTAFGPFRLFPTQRLLLDGDKPVRLGS
                     RALDILIALVEHPGELVSKEELMARVWPSTIVVEGNLTVHVAALRRALGDGHSGNRYL
                     VNIPGRGYRFVAAVALAEQRGPSPARIPPARHRQNLPALLTRLVGRAEVVSSLVEQLS
                     RHRFLSIVGPGGIGKTSVALAVAEKLIQVHEHGVWLVDLASVGDPHLVPTALATVLGL
                     EIRSENPLPGLIADLRDKRMLLLLDNCEHVIDAAAALAVGILKGAPGVRVLATSREPL
                     RAEGERVHRLSPLESPPVSARLTAAEALDFPAVQLFVERAAATLDEFELSDADAAIVA
                     EICRKLDGIALAIEFAAGRLDVYGVRGLAARLEDRLRLLTSGRRAALPRHRTMSATLD
                     WSYCLLGEAEQAVLRRLAVFSGDFTLRAAGAVAADMTHSEDQIIEQVTNLVTKSLVTA
                     DVSGAEPRLRLLDTTRAYAFEKLSESGESDTLGQRHATYYLNLLELAAQDKTAADGWP
                     AIFVPEIDNIRAALAWAFWPGGDASIGARLGAASAPVWFEMSLLTECHDWTVRALGAL
                     DTEQVGTRHEMVLQATLGLSLMYTTGMTTDGHAALAKALDLAEKLDDFDYQQRILNGL
                     WMYNLRRADFRGALSLARRLEALAQEFNDPAAMLTAYWTLGISLHFLGDQHQARGHLQ
                     RALGKQASWSQRTDIVRFGVDRPTSALSVLANTLWLQGFPDQAVQTGRRSIVEAQALE
                     HPVSLCLALKWGGSAVSLRIGDLVSAESSITELIDHSERYSLHTDYACGIGLQGQLSV
                     KRGDVLTGIRLLRSGLGGLHEAHFQVLYTEFLADLAGALAADGQVDEGLAAIGDALQR
                     SDRSDELWYMPEVLRIKGEILLLQNRSNAAAAEDHFLRSLDWARRQGTLSWELRTAIS
                     LARLHKEQRLIERARDLLAPVYARFTEGFGTVDLLTAKRLLGELT"
BASE COUNT         3283 a         5440 c         5338 g         3180 t
ORIGIN      
        1 aagaacacca tgatcaagcc gtgggccgtg attatgacgt tgtagagctg cgtgtcgcca
       61 tggagcaggt ggtcgccggg ccgcatcagc tcgatgcgca tcagaaccga tatgaagccg
      121 ccgatgaggc cggcgccgat ggcgaaggcg agatagagcg taccaatgtc cttgtggttg
      181 gtggacagca gccagcgcac ccagaaagag ggccggtgat cttcgtgcgc atgctcatct
      241 gctctagcgt tcaatccaca cgccatcgtt ctatccccgt cagaatgctt accgagcggc
      301 gaggtgctat ccggcgtcgc tgccgttcgg caccacaatc aacgaggcct ttttctgcgg
      361 aacgttccag agcgagtccg gattcttgat ccagcgattg aagtcaccct gcgacaccgc
      421 tttgacctcg atcggcatga agggatggtt catgccgcag atctggttgc actggccgta
      481 gtaggtcccg gggcggtcga tcctcatcca gctttcgttc ttgcgaccga tgaccgaata
      541 ctcctgcacg ccgagcggag ggacaaacca actgtgtatt acgtctgtgc cgccgacgat
      601 gacacggact gtggcgccga ctgggacgat cattggcttg tccacaccga gcagacgcgg
      661 ctcgcgatat gtcgcgaggt cgtcgtcttg cagcatcgtg ctgtcgaagg tgagaccgcc
      721 ctgatccgga tattcgtagc tccagtacca ctgatggccg atcacgttga cggtgaaatc
      781 ggccgtccgc acgcggtcgg cgtaatagag gagcttgaac gacggaatgg cgatgtagac
      841 caggatgagg acaggcaccg tcgtccaagt cacctcaatc aacctattgc cggtggtcgg
      901 cgatgaaatc gggtggcgct tagcgccgaa gcgcacaatg atcacgacga gcaaagccag
      961 gacgaaaagc acgattacgg tggcgatgac cagaagtttg tcgtgcagag agtcgatctg
     1021 ttcgcgcacg ggcgaggcgg ccgcctgcat gccaagttgc cagttaaccg gctcttcggc
     1081 cagcgccgcg ggtgagatca ctgcccaagc gagcagcgtc agcgccgcgc cgatcgcgag
     1141 agtcccacgc ttgatatcaa cgctccaacc cgatcccatg cccttgtact cccgtatgga
     1201 cagcgtccgg ttcgcgcgtc gtgtccggtt tacccagatc gtggcatggt tcggacttca
     1261 ggaccggcgg tcgtaacgcg gcgcagatgt cacttcttct tgccggtcga gtcgaattgc
     1321 ctcagatagg cccaaaggtc gctgacgtct tggtcgttct tgatgccggc gaataccatc
     1381 ttcgtgccag gcaacttggc cttcggatcc ttgatgtagt ccctgaatga tgcgtcgtcc
     1441 caggtaatgc cggatttctt attggcgtcg ctgtagctgt acccctggac gctggcggct
     1501 ttgcgtccgt cgagaccgtt aagctcggga ccgatcttgt tcttggcgcc ttcgccgacg
     1561 gcgtggcagg ccgcgcattt cttgaaagac tgctcgccgg ccgcgacgtc ctgcgcgaga
     1621 acggctccgt tccatgcaag ggtcaccagc agcacaacca ctacgctttt catcgctctc
     1681 gctccgtttt ttgaagcgtg cctgtcgctt ttagatatat cgcaccacac gcgagggccg
     1741 agtgaagggg gccaaatctt gccaaatcct tgtgaatggt ggtcgctgat ggtccggttt
     1801 acctggacga ggttcgctac cgttgcgaac cgcgggtctg cggccttccc gtcgtttaag
     1861 acggatatac cgcgatgaac ctcgcggcag gcacgccgat tgccagaagc acaaccacga
     1921 ctgcgccgat gcgctccgcc gcgttccaac ggcgccaatc ccgacgcaga tcctgcagcg
     1981 ccgacacggc cctaatccat ccacatgatc attgccgccc gagccacgat tgcatcacgt
     2041 cgccttttcg ggcgcaccga atttgcatcg ccatcccctg agcgcccact tcgggtgctc
     2101 gtcaagccac tgcgacgcga gctgctgcgc ttgcaccatg caggacggtc cgctcacgga
     2161 cgcgatgggc cgccgctctt ctcggcagac ctccggtgca gcggtgaggc agacgaccag
     2221 gattaaagac accattgcgc atctccgatt tcgcgtttca ctgcgctctc tctctttttc
     2281 cgcggttacc gccgtctcga tttctctcgt gcgctcgtgt cattgggcga aggcaccgac
     2341 cgccgcggcc ggctcgacgg tgccgtcggg cagacgggcg atgttgtcgc aattgagaca
     2401 cttgcccggc accgcgggca tctccagcgc caccggcacg acgaggtcga acgtggcgag
     2461 gccgatggcg atgaccacga gccacgcatt gatctcgccg acgatgcgat ggaggcgttt
     2521 tatcgaatga tcggacatcg tggtgacctc gggattgccg gcgggcttca gcgatacccg
     2581 atgtaatctt ctacagcgtc tcacacaccg aaccgagtga agcgcggcaa gccttggcaa
     2641 attttggtaa atagagccgc gcacgacgct cgcctaaccc gcgccggcga gagagacgag
     2701 aagatccccg atacgaggtg cctggctcag gcaccgatat gagccccctc gccttccttc
     2761 atcaccacgg cgcaggtcat gtcagcgcgg tcagcacctc ggaaaggggc gtccgtcaga
     2821 cggatacgga ctggatgcgt tgcgccaacg tcatccaggt gaatgtcgga tgacgcatac
     2881 ggccacggct tgcccaacgc gagcgaactg ccggcgttgg cgtaatttgt ccgcgacaaa
     2941 gcccgaaaat ccgccgacga gcctgctggc gattacaccc gaaatccgtc tctacggcag
     3001 gtcgatcatg ggcgtgccgc ggccggcggc tgccggtggg agccatttct gaagagcgat
     3061 gtgatcgccc attcgcgggc gggcgcagcg acgacgacgg tgcaggtcat gcctgcgctg
     3121 atgcgcacgc tctctggaat ctcgtcgatg tggatgcgta ctggtatgcg ctgcgccagc
     3181 cgaacccact cgaaggtcgg gttcacattc gccagaagct ccgggccgtt cacgttgtct
     3241 cgatccgtga tgccacggct gatgctctcg atgtgccctt gcaaggcagg ttcgccgctc
     3301 atcagatgaa tctcgacccg cgctcccggc ttgatgacag gaatctttgt ctcttcgaaa
     3361 tagccctcga cgcggtagga gtcgctgtcg atcagcgcca tcagcttggt cccgaccgcc
     3421 gcgtactggc cgatgacgac cgggttggtg acgaacccgt ttacgggagc ccggatgacg
     3481 gttcgctcga ggttgaggct ggccgtcgac cggtccgcca cggcctgttg gtatgccgcg
     3541 gtggccgagg ccgcgacgtg ctgggcgtcc tccttggcct cgggcgagat cgagaggttc
     3601 gtcagcttgg cgcgccgatc ggcatcgccc tgccgcatga gcatctctgc cttgcgggcg
     3661 gccacactcg cttccgccgt cgccgcggcg cgcttgtaac gttcctgatc gaggatcaac
     3721 aggatgtcgc ccttatggac gagctggttg tccttgacgc gaatatcatt cacgaaccca
     3781 gccacgtcgg ggacgatcgt gaccacgtca gcgcgcactc tcgcgtcgcg cgtccaaggc
     3841 gagagcatgt agtagttcca gagctcgtag gcgcctgcgc aaccagccgc aacgatcaga
     3901 agcgtggcga tcacgcgcgc gagggttttt atcgttgcca catcagggtc tcgttgcaag
     3961 cacgaggacg gagaacacgc agaggaagag accgaatcga gcgagcggcg gatgccacaa
     4021 gagccgatag acgcccaggc gggcgatcag ccggtcggcg acgacgaaca gcaggatcgc
     4081 ggcgaaaaag tagagcaaga gcgacggcgc gagcgcgcca aagagcgcga gctcacgcaa
     4141 catgggccgg cgctcccgtc gctctctcat ccaggaggag ctcgccgccg cgctcgagtt
     4201 cgtcgcggat agcggcgaag gcgaccatct cgcggacagc cgtccgcgct tccgccacgc
     4261 cgaccctgtc gtctcgcaat tccgccaggc acgttactga ggcgtcctgc gcggcacgca
     4321 gcgcgcgatc gagcgtcagc tttttgttgc tggcgaggaa ccggacgact ttatcctcca
     4381 cgtccatctt ggcaggcgtc ggccggccgt cgtcccgaac tcggatcagc tcgcgtccgg
     4441 tgatcaggag agccgtgccg gcgtcgagag ccgcgaggtc ggccggacgg tcggggcgga
     4501 gatggccgcg cagccgatcg agagcttcgg tcattgccgt ctcgaactct gccaggccga
     4561 tacgcgggcg tggcgccgca attcttgcca gcagcttccg cgcggcgcgc ccgaagcggc
     4621 ggtgggccgc ttcgggcgtc tcgggcgcga cgatagccca tagaatcatg gcaaccgccg
     4681 cggcaagggt cgccgctatc gacgtgttga cgagcccgat gggatcataa accattcggt
     4741 tctggaacgc cccgaccgag gcgaacaaga gcgccgacaa gaaaccgatg accatggttc
     4801 gctcgtgcgc catcatgaaa gcgcaaagaa aaagcatcgg tgcgaccact agggcgaaca
     4861 tggcgaatcc cgaggcaagc ggtagcagcg tttcgacgag aacgaaggcg gggatggtcg
     4921 agaggctgaa gatgagcgtg gcggcgatgg caaggggcac ggcgtgcccc atggtcgcga
     4981 gccgcgccgt cgccacggca gcgaggatta ccgctgtagg cccgtgcggc cagttggcgg
     5041 cgatccagaa cccgctcacg agcaggaccg caagcgcggc acgcagcccc gtccagacgg
     5101 cgctggcgaa gtcgttggcg aggccaaagt cgacgcggcg atttgagcct ggctcgccgg
     5161 cggcgaaagc ctggtcagcc tcggcataga cgaccatggc ggcgaagaac tcgcgcaggc
     5221 gcccgatgac ggcggcgcac cggatgacat cctcatccgc ggcgaacggc gccaggcaga
     5281 gttcccgagc gagcggcaag ctggcgctcg cctgcaccag gcgccggctc aagccggccg
     5341 cgtcgagctt gccgccacgc caaacgtcga tcgcggcggc cgctcttgtc aaggttccgt
     5401 ccaggccagg gccgattacg gcacggccgc gccgaagcca gtccagcgat cgaccgagga
     5461 ggtgcgcgac gtcaacgacg ccgagcatcg cgatgtcgag gcgccgaagg acgccgcttc
     5521 ggttctggat ctcgcggtcc tcaaagaccg ccgacgcgcg cagattctcg atcgcgacga
     5581 cctgaccgag cagcttggtc cgcagcgagg tggtgtcccg gccgccgagc agcagcgccg
     5641 ctgcgtagtc ggcgaggtcc gctcggccgg ttgcaacggc gcggcgcaag gcgccggcga
     5701 gcgacatcgg caggacgaga tgactgaccg ccgcagccgt catgatgccg agcgagatct
     5761 cgctgacacg agcgaccgcg atgaagaaag cgtttcccgg gtcgagtgcg cccggaatcc
     5821 cgacgatggc cacggtgtag ccggaaagga cgaatccgta agcggcccag ttcctcgcgt
     5881 attgggagcc gaaggtgcaa aacgctatcc agacggcgag agcgccaagg aagaggacac
     5941 gctcctgggc gaagagggca acgagaacga gcgcgccgcc agcgccgata accgtcccga
     6001 tgatgcggta gaagcttttc gcgaggacca gcccgtcctt cggttgagaa acgataaaga
     6061 cggtgagcag cgcccattgc ggctggtcga ggttgaaggt aaaggcgacc agcagggcga
     6121 gcaggcccgc ggccgtggtc ttcgcggcga atatccacgg gatccagcgc ggaccgaaga
     6181 accacctcgc cggatcggtg tccggcacag ctccagacga tggaggggcc gggccgagct
     6241 cggcttcggt cgctgtcggt ccgcgtcgcg caccagggcc agagctcaca gcgacaagtg
     6301 agcgcccttc aggcgagaca ataggctcgg ggaccgcggt cggcgacgac gcctttgttc
     6361 ctgtgctcga aagcgtggcc gtcattcgcg gccttcctga gcgagatcga gtatgaatac
     6421 accatcggca ctagcagggc gcttgagcac ttcaggatgc tgcaatgtgc gaacggtgtc
     6481 gtagtagccg gcccgccaat ggtcctccat gctcatcagc gagaattcat agtccttcga
     6541 gtcgccttcg tactgtttcg cccgatagac caactcaacc agattgtaga ccttatgctc
     6601 ggaagccggt ttcaatatct ccacctccgg catgctcctt agctcctccg gcaacttctc
     6661 gagaaggctc gccacggcag cgcgcagcct ctggatctgc ttgaagcggt cggagttcgc
     6721 ccgagtccgg ctcgagtatt gaatctcctt ctggcgtgtc gtcacctccg gcaggttgcg
     6781 cgggaattcg ccgcttgcgc tccagagatc gacctggaag gccaacgtgt cccgctgcgg
     6841 ctcgctttgg acaacccact gcaatggcgt gttggagacg aggccgccat cccaatagcg
     6901 ctcgccatcg atctcgaccg ccgggaaacc cggcggcagc gcgccactcg ccatcacatg
     6961 ctcgggtcgg atggcgtgcg aggtgttatc gaagtagacg agattgcccg tcgtcacgtt
     7021 gacggcgccc atgctcaagc gcatttccgc ggcatcgatg cggtcgaaat cgatcagccg
     7081 ctccaatgtt gtcctgagtg cgccagtatc ataatagctc gtcgccccca acgagccgtc
     7141 ggggcatagc catggaaccg gcaagcgcgg cgtgaagaag ccggccgcac cggtcatcag
     7201 cacgcggccg gcgctgagct tattgaacag accgcgcgcg gcgtcaccct tcaccgtctg
     7261 cagctcgaga ccgtgaagcc cactgctcac tgccgccgag ccaacacgct cccagaattc
     7321 ccacatcttg gctgcgtagg tgccctgcca cgcatcgggg ccggccgttg gctctgcgac
     7381 catcgccgcc tgggacgcat gcttcaaaag cggaagtggc gtcgtgatct gctgccagaa
     7441 ttcacgcagc ttttcgaccc gcgattccgg cggattgccg gcgataaggg cggagttgat
     7501 ggcaccgatc gagattcctg ccgtccaatc gggatgcaga ttcgcttctg ctagcgcttc
     7561 gtagactccg gcttggtagg cgccgagggc gccgccgcct tgcagcagca gcgcaatgca
     7621 atcgaatggc ggccggcttg ggcgacgcgg taagttagtt tttgtcggct tggccatgcc
     7681 ttgggctcct agatgaggcg gcgctatccc taagggagag cgaatacgat cacggccgaa
     7741 gtgcccgtgc cgccgaagaa gccagagacg acgcccgatg tcgtcgcgac atactgcttt
     7801 ccgtcgagcg cgtaggtgat gacaccgccg ccaatcgagc cgccggtgtt gaagcggtac
     7861 agcgtgtttc cctcgcgcgc gtcgatcgcg agaaagtcgt tgttgagatc tccggtgaag
     7921 agcacgccgc ccgaggttgc cgtgatgccg gcgaccagcg gcgtcggcca gtgctggcgc
     7981 caatgcaccg agccgctcgc cgcgtcgatc gcgtagagcc aacccttcgc ctgatcgcgt
     8041 gcgtccgggg tgaccgagcc gccgtaataa tgcgcgttct cggcgaattg cggtgggccc
     8101 tctgttctct tgaaggtgcc gcaccaatcg accgtcgcga cgaacaatgt gctcgtcctg
     8161 ggattgtagg caggtccgtt ccactccatt cctccgagca ggcctgggca tacatgcacg
     8221 cctgcgacgg tcggaaccgc ctcggggttt tcccgcgtgg tgatcggcat ctcgtagaga
     8281 agttcgtgac tgtcgcggtc gaccatgcgc agcaggccgt ccttgccgct aatggtcagg
     8341 aggtttcgcg gcttgccgtt gaccgccgcc cgaaagatcg ggctgacctg gctcaaatcc
     8401 cagtcgtgca tatcgttggc gcggaactgc tgataccaga gcagctttcc cgtcttggca
     8461 tcgagtgcga cgaccgagtc ggtatagaga ttggtgccgg ggcggacctc gccgttgaaa
     8521 tccggtgagg gattgccgac cggcacgaac agcacgccct tttctgcgtc gagcgacaac
     8581 ggcgtccaca ccgcgccgcc gccgtgctcg cgggcctgcg gattggtcca gctatccgct
     8641 cccgcctcgt tcgcgtctgg aatcaagttg aagcgccaga ccggctcgcc ggtgtccagc
     8701 ttgaacgcgc cgatccagtt tttcgcgccc caatcagcgc cggccgggcc gtagatgatc
     8761 aagtcgccga aaatgagcgg cggcatgctg aggtattggc tcgccttcgc gtcggcgatc
     8821 ttgcggctcc acagcagcgt gcccttgtcc atgtccaccg cgaccagata accgtcggcg
     8881 gtgccgcgca cgacccggcc gtccttgagc gccacgccgc gattggtcgc ggacagcact
     8941 tgatccttgg tctgccaatt gtaggtccag cgctcgcggc aggtcgccgc gtcgatggcg
     9001 acgatctgct tgtcgatcgt caggtacatc actcccttgt acaccagcgg gttggtttgc
     9061 gtcgcgccgg cgttgttcga gcgatagata cagacggcgc ggaggctggc ggcattgctt
     9121 gcattgatct gatcgaggtc gacgaagcgc tggcccgtgt agtccttgct ggcatagagc
     9181 cagttctgcg tgtcgccggc cgcgtgcagc aactcctcct gcgtcggtcc ctcgccgagc
     9241 ggcatgtttg caggcgccgc cgacgtggcg ggtgcgttag ccgcagcggc cactcccagg
     9301 gtcgcgagat acgcgattac attggcacga tctgcctcgc tcgggatctc gaccgacatc
     9361 gatgttcccg gcactttttt tgtcgtcgag gcgaggaatc catcgagcgt cttggcgtcc
     9421 caggtgagcc cggcattcgc catcgcggtc gagtagttgt aacccgccac agcgccggac
     9481 tttctgtcga tgactcgagc caaggacggg ccaaatccct cgccaccagg caccgtggaa
     9541 tggcaagacg cgcactgatt ttcaaagacc gtcttacctg ctgtcgcatc acccgctgca
     9601 taggcgccat tgctggaaag cacggccgtg cccaggacaa accaaccggc gaggttacgc
     9661 atggcacttc tcctcggact ctctttacct tggtaccggc gatcacgcgc cggtcgcggt
     9721 cacagcagaa agaactccag cagcaacagc acggagacaa cgagcgcgac acccccctgc
     9781 actatcctgg tgaaaccctg ccaggtctcc tggtggcgtt ccagctcctc gtctacggcc
     9841 atcacaagtc tccctaccta acccatcgcc ggtcgcgtga cgaccagtca gagatgagcc
     9901 tgggcgcgaa cattcggctt gcccgcgctt ggccgctctc cgcgtctcga ccgtttcgag
     9961 gggcttcgcg ccgcatgcat agaccgccgc agtttttcca gcgctcgcgc ctcgatctga
    10021 cgcatacgct cgggcgagac cgcgtaacgc ttgccgagct cgcgcagcgt cgtcggcttc
    10081 tcccggagac ggcgctccga gatgatgtcg cgctctcttg ggttaagatg ctttagagcc
    10141 agattcaggc gatgaagacg ttgcctcagc tcagtccgct cgccgacgag cgtctcctga
    10201 tcaggtgaac tatcgggcaa tatgtcgagc cattcttcgc cgtattcgga gatcggccta
    10261 tccgacgagc cgttgagtga cagatcggga ccggcaagac ggtgattcat ctcgatgaca
    10321 tcggcttcgc tcacgtcgag ccagccggcg atcttcctag cgttttcggg cgagagctcg
    10381 ccatcgtcgg gtgcctgaag ctgctccttg cagctcctca gaccgaaaaa tagcttcttc
    10441 tgcgcagtgg tcgtccccat tctgaccagc gagctggaag acatgatatg tcgctggatc
    10501 gcagcacgga tccaccagac cgcataggtg gttaaccgga aaccgcgctc ggggtcaaag
    10561 cggtcgactg cctgcatcaa gccgacattg ccctcggaga taagctcact gagcggcaga
    10621 gtgtggctgc ggcagcattt ggcgattttg acgacgagcc ggaggtggct gctgaccagc
    10681 cgctgcgctg cgccgctatc accgtgctcg cgccatcgct tggcgagcag atattcttct
    10741 tcgggctcca acatcgggaa ctcccgaatt tgccgaagat aaggcgcgag accgtcaaac
    10801 ggacccgctg ctaaggtgac ccctctcgtc attgccgact tctccagctt gccctaggcc
    10861 gccgccttta agcccagcca gttccacacg tcgcgcaggg caaacacgag ccgctcgatg
    10921 tcgtggtcgg tgtgcagcgg cgtcggcgtg aggcgaagtc gttcggtgcc gcgcggtacg
    10981 gttgggtaat tgatcggctg aacgtaaatg cgatggtgaa gtaaaagctc gtctgaggcc
    11041 gccttgcaca acgaggcgtc gccgaccgcg accggaacga tatggctctg cgacggcatc
    11101 accggcagac ccgctctggc aagctctgtc ttcagccttg cagcgcgctc ctggtgccgc
    11161 gcacgtaccg actggtccgc cttgaggtga cgtacgcttg cgagtgcccc tgccgcgatc
    11221 gccggaggca gcgccgtggt gaaaatgaaa cccgacgcat ggcttctgag gaaatcaacg
    11281 agtgccgccg atgcagccac ataaccgcca atgaccccga ttgcctttgc aagcgtgccc
    11341 tgtattgcgg tgacacggtg catcaggccc tcgcgttcgg cgattccgcc gccccgcggt
    11401 ccgtacaggc cgacggcgtg cacctcgtcg agatacgtca tggcgccgtg gcgctcggcc
    11461 acgtcgcaca gctcggctat gggtgcgatg tcgccatcca tcgaatagac cgattcaaag
    11521 cagaccagct tcgggcaggt tgggtcggcg gccgccaaca gccgatcgag atcacggacg
    11581 tcgttgtgac gaaatctata tttttcggcg ccaccatgtc ggatgccttg gatcatggag
    11641 gcgtgattga gggcgtcgga aaagatcatg cagcccggga tctggcttcc tagagtggcg
    11701 agcgccgcct cgttggcgac atagcccgaa gtgaagatca gcgcggcctc cttgccgtgg
    11761 agatcggcaa tttcccgctc aagcagcacg tgatgatgtc cggtgccggc gatattccgc
    11821 gtcccaccgg cgcccgcgcc gatgctatcg atcgccgcgt gcatcgccgc aagaaccgcc
    11881 ggatgttgtc ccatgccgag atagtcgtta gagcaccaga ccgtgacctc tgcgcccaag
    11941 ccgtggtgaa aggcgcgtgg aaatgccccg gcccggcgct cgaggtcggc aaacagccga
    12001 tagcgccctt cggtccgcag cgtatcgagc cgtgctttga agaaggcttc gtagtcgtac
    12061 atgagcagtc cccgatggta gatatgtcgg gaagcgattc gtggcgtcag ccacctcgat
    12121 actggccaag tgctacggca tatcgtgcgc accgccgagt gaagcctgcc aaatcccggc
    12181 aaatcgatgt aaagcggaac cgagattgcc gggctcggcg cgtttccgac accggggctc
    12241 cggctcataa ccgcggtaat cgtatcggcg taaaactctg ccttggatcg tcatcggggt
    12301 gtcgaccgcg ttgggcgcgg ctagacgaac gcactgaagc ccgtgatggc cttgccgacg
    12361 acaagcgcgt tcatttcccg ggttccttca taagaataga gcgcctcggc gtcggcggcg
    12421 aaacggccga tgtgatggtc gagcaggatt ccgttggccc cgagtagttc ccgtgcccag
    12481 ccgacggtct ctctcattct gacgatgcaa atgcccttgg ccagggaggc gtgctcgtcg
    12541 cgcatgagac cctgatcctg cagctgagag agccgcaggc agagcgcctg catcgacgtg
    12601 atgttgccga gcatcctgaa cagcaggtcc tgaacaagct ggaaccgcgc gatccggcgt
    12661 ccaaactgcg accggtgctg ggcatatcgt agcgcgtgct cgtaagcccc ggttgcacag
    12721 ccaacagatt cccacgcaac ggccgcacgc gccaggcgaa gcacattcgc ggtatccttg
    12781 aacgagttgg ccttctttag tcgatttgct tctccgacgc ggcactcttg gagagtgatg
    12841 agcccgctct gaacgacccg gagcgccatc ttgttttgta ttttctcgac gtggagccca
    12901 ggcgagccct tctcgacgat gaaacctttc acctggtcgt ccgcgacgtc acgcgcccac
    12961 acaatcgtta cgtcagccca ggtggcattg ccgatccatt tcttctgacc agacaatacc
    13021 caggtatctc cctcgagccg ggcggttgtt tgcaggcccc gcgacacggc agacccaacc
    13081 tcgggctcgg tcaaaccgaa tgagccaacc tttcgaagcg ctgccatttc gggcaaccat
    13141 ctctgcttct gctcctctga accgcagaga tagatcgagc ccatcgcaag tccggtgtgg
    13201 gcgccaaaaa atgtcgcaat ggatgcgtcg acccgtgcaa gctccatcat gacgaatccc
    13261 atcaatgtcg tgctcctgcc ggggcagcca tagcccctga tgggaaggcc ggcgatttgc
    13321 aattccctga acttcggcag cagatcgaac gggaattctc ccgttgtcca gaactcattg
    13381 atgatgggct ggacctcctc ttccatgaag gtccgcacct tgattctcag atcgttgtct
    13441 tccttggaaa ggatctttgc caccagataa aaatcgccat ctggttcggg aagcgcttgc
    13501 gcggaggacg tcgagtcaag cggtgcggac gccatgctgt tgtcctcttg caattaggga
    13561 tgaaaggaac tgggggccgc cactgcgtca gcgcgggaag ccgcgccgag attctgcagc
    13621 ggactgttga ggcgtgatct gacgtaaaac cacatgcttc ccggatcctg gatggacctt
    13681 gttacatgcc gtcgcaggcc cggaggaatg aagtctgcca aatcacagca aatgtttcta
    13741 aaccgtctat catcaagctc gtttggctca ttggaccttg cggaatagtc gccccttgtg
    13801 aatgggcgga ttgaagaaaa ggagccggtg attctccacc accggctcca gtgcctgcgc
    13861 ctcgttcgaa atccgcaggg aggcgcgcgg tctcgaaagc caccgtcggg ggaaggcagg
    13921 acctggggga aggcctgggt ggacgaagcg cagtatctga agacttgcga aatagccatc
    13981 gagcacagaa tacggaaccg gggtgctaac tccgtgcggc gccggagccg acgcgatccc
    14041 gcgttcccga tctaattaca atcgcacccg agcgtcaggg cgaatgaagc ttgccaaaca
    14101 ttggcaaatt tctgtgaagt gatcggactg atcgctacgt caattcgccg aggagccgct
    14161 tggccgtcaa cagatcgacg gtcccgaacc cttcggtgaa gcgtgcataa accggtgcga
    14221 gaagatcgcg cgcgcgctca atcagccttt gctccttatg cagacgggcg aggcttatgg
    14281 ccgtccgcaa ttcccacgac agggtccctt ggcgacgcgc ccaatcgagc gagcgcaaga
    14341 aatggtcctc cgcagcggca gcatttgacc tattctgcaa gaggaggatc tcgcctttga
    14401 tgcggagcac ttccggcatg taccacagct cgtcgcttct gtcgctccgc tgtagcgcgt
    14461 ccccgatggc agcgagaccc tcgtccactt ggccgtcagc agcaagagcc cccgccaagt
    14521 cggccaagaa ctccgtatag aggacttgaa agtgggcttc atgcaatccg cccagaccgg
    14581 agcggagcag ccggattccg gttaggacat caccgcgctt cacggacaat tgtccctgca
    14641 gcccgatccc gcaagcgtag tcggtgtgca aggagtatcg ctcggaatgg tcgatgagtt
    14701 cagtaataga cgattctgca ctcaccaagt caccgatcct gagtgaaacg gcgcttccgc
    14761 cccatttcag cgcgaggcac agggacaccg gatgttcaag cgcttgtgct tcgacgatac
    14821 tcctccggcc cgtctgcacc gcttgatcgg gaaacccttg cagccaaagg gtgttcgcca
    14881 acacgctgag tgcagatgtc ggccgatcca cgccaaagcg gacaatgtcg gtccgctgcg
    14941 accaagatgc ctgtttgccg agcgctcgct gaaggtgtcc ccgcgcctgg tgttgatccc
    15001 ccaggaaatg tagtgaaatt cccaaggtcc agtacgccgt cagcattgcg gccggatcgt
    15061 tgaattcctg tgcaagtgct tccagccggc gggcgagcga aagggcgcct cgaaaatcgg
    15121 cgcggcgcag gttatacatc cataggccat tcagaatccg ctgctggtaa tcgaagtcat
    15181 cgagcttctc ggcaaggtcg agcgcttttg ccaatgccgc gtggccgtcg gtcgtcatac
    15241 ccgttgtgta cataagcgac aggccgagcg tcgcctgcaa caccatctca tggcgtgtac
    15301 ccacctgctc ggtatcaaga gctccgagcg ccctaaccgt ccagtcgtga cattcggtca
    15361 acagcgacat ctcgaaccat accggagcag aagccgcccc gaggcgcgca ccgatcgatg
    15421 cgtcaccccc aggccagaat gcccaggcga gcgccgcgcg aatgttgtcg atctccggga
    15481 cgaagatggc cggccagcca tccgcggccg tcttgtcctg cgccgccaat tcgaggaggt
    15541 tcagataata cgtcgcgtgg cgttgtccga gcgtgtcgct ttcgccgctc tctgagagct
    15601 tctcaaaagc ataggcacgc gtcgtatcga gcagccgcaa gcgcggctcg gcgccgctca
    15661 catccgctgt aaccagtgac ttcgtgacca agttagtcac ttgctcgatg atctggtctt
    15721 ctgaatgggt catatcggca gccacggcgc cggcagcgcg aagggtgaag tcgccagaga
    15781 agaccgccag gcggcgcaag accgcttgct ccgcctcgcc gagcaaacaa tagctccagt
    15841 cgagcgttgc gctcatggtc cggtgccggg gcagagccgc acgacggccg cttgtcagga
    15901 gccgcaatcg atcctccagc cgcgccgcca ggccacggac cccatagacg tcaaggcggc
    15961 ctgctgcgaa ctcgatcgca agcgcaatgc cgtccagctt gcggcagatc tcggcgacga
    16021 tagccgcatc cgcgtcgctg agttcgaact cgtccaaggt cgccgcagct cgttcaacga
    16081 agagctggac ggccgggaag tcgagcgcct cggcggcggt gagccgtgcc gaaactggag
    16141 gactctccag cggcgatagc cgatgaaccc gctcaccctc cgcccgcagc ggctcgcggc
    16201 tggtcgcgag gacgcgcacg cctggcgcac ctttcaggat ccctacggcc aaagccgcag
    16261 cggcatcgat cacgtgctcg caattatcga gcagcaggag catccgcttg tccctgagat
    16321 ctgcgatcag acctgggagc gggttctcgg agcgaatctc gagcccaagc acggtggcaa
    16381 gcgcggtcgg aacgaggtgg gggtcaccga ccgatgccag atcaaccaac cagacgccat
    16441 gctcatgaac ctggatcagc ttctcggcca cggcgagagc gacggaggtc ttgccgatgc
    16501 caccgggtcc aacgatgctg agaaatcggt ggcgcgacaa ctgttccacg agactgctaa
    16561 cgacctccgc gcgaccaacc aaacgcgtca gaagggcggg cagattctgc ctatgccttg
    16621 ccgggggtat tcgggccggc gagggccccc gctgttccgc gagcgcaact gctgccacaa
    16681 agcggtagcc tcgaccggga atattgacca gataccgatt gccgctatgg ccgtcgccca
    16741 gcgcccgccg caaggcggcg acatggaccg taagattgcc ctccacaacg atcgtgctcg
    16801 gccaaacgcg ggccatcagc tcctccttgc tgacgagttc gccggggtgc tcgaccaagg
    16861 cgatcaggat atcaagagcg cgactgccga ggcgaacggg cttgtcgccg tccagcaaca
    16921 gccgttgcgt cggaaacagg cgaaaggggc cgaacgcggt tgagcgctca atcgagaccg
    16981 caggttctgc aaattggagt gcaccgtcgc gcataggccg ttccccacta gcctcggtcg
    17041 ggcgccgctg acaaaaccga gcgcccagga tctcttccct acccgcgact attggacgta
    17101 cactcttccg gcaaccgctc ccatcccccg aacgggggat cgagggaccg ggtttttggg
    17161 ggagcgcagg gaggcagacg gcagcgtcgt gctcgattcc tcggcgagat tctgtcacca
    17221 gctactcatg accccaatga t
//
LOCUS       DASWGM010000525         5083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_316002,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000525 DASWGM010000000
VERSION     DASWGM010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316002"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /gene="hisS"
                     /locus_tag="VGA15_15285"
     CDS             complement(<1..1294)
                     /gene="hisS"
                     /locus_tag="VGA15_15285"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX9589097.1"
                     /translation="MAEKPKKPQKLRARLPRGLADRGPAEIAATRAMVEKIREVFERY
                     GFEPVETPAMEFTDALGKFLPDQDRPNEGVFSFQDDDEQWISLRYDLTAPLARYVAEN
                     FDALPKPYRSYRAGYVYRNEKPGPGRFRQFMQFDADTVGSASPAADAEMCMMAADTME
                     ALGIPRGSYVVKVNSRKVLDGVMESIGLGGDENAGRRLTVLRAIDKLDRLGEKGVREL
                     LGEGRKDESGDFTKGAGISMSDIETVFAFMHGGSDANDKSFFQRLENVFNEKGSTTGN
                     EGLKELSQIFDIIMASDYGDGRVLMDTSVVRGLEYYTGPVYEVELTLETKDEKGRPVR
                     FGSVGGGGRYDGLVSRFRGEPVPATGFSIGVSRLQAALTMLGKLDTKPEFGPVVVTVF
                     DRDRVADYQKMVAALRAAKIRAELYLGNPKNMGNQLKYA"
     gene            complement(1545..2435)
                     /locus_tag="VGA15_15290"
     CDS             complement(1545..2435)
                     /locus_tag="VGA15_15290"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX9589098.1"
                     /translation="MGVSFDKIDGVIWYDGKLVPWGDANVHVLTHGLHYASCVFEGER
                     AYGGTIFKGTEHSERLKNSARILDFEIPWSVAEIDAAKRLVLEKNNLPEAYLRPVAWR
                     GSEMMGVSAQTNTIHLAIACWTWPSYFDPAERLKGIRLGLAEYRRPDPATIPALAKAA
                     GLYMICTISKHKAEREGYADAMMLDWQGRVAECTGANIFFIKDGKIHTPTADCFLAGI
                     TRATAIDLAKRRGIEVIERRIMPEELSGFSECFITGTAAEITPVSEIAQWKFKPGAIS
                     EQLMAGYTAEVQPKGKAAAA"
     gene            2748..3308
                     /locus_tag="VGA15_15295"
     CDS             2748..3308
                     /locus_tag="VGA15_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9589099.1"
                     /translation="MADVNLSNPSSDPDSRATEAPSAAPRSGDLRWDIIELLFFAYRD
                     FVGDADHELEAFGFGRAHHRVMHFVHRYPGLKVADLLDVLRITKQSLGRVLKQLLDEG
                     YIVQKTGNNDRRQRLLFATAKGEALVGKLAGLQTDRINRALRDIGPSEAEIVRQFLRA
                     MIDHDDPDKVLATILGAANATATAKE"
     gene            3313..4035
                     /locus_tag="VGA15_15300"
     CDS             3313..4035
                     /locus_tag="VGA15_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9589100.1"
                     /translation="MAQAATLARKPPQPADDAPHVLLVDDDRRIRDLLSRFLCGEGYR
                     VTTATGAQDARAKLLGLHFDLLILDVMMPGETGFELARFIRTSSSVPIIMLTARHEAE
                     NRIEGLQIGADDYVAKPFEPRELVLRIGNILKRAAPPPVETLEQAAFGPYVYHLDRGE
                     LRQGDEVIHLTDREREMLRILAIARGETVPRSALTGDGTVNERAVDVQINRLRRKIER
                     DPANPLFLQAVRGIGYRLVASP"
     gene            4062..>5083
                     /locus_tag="VGA15_15305"
     CDS             4062..>5083
                     /locus_tag="VGA15_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9589101.1"
                     /translation="MSTLDTGLTLIRTAAGRVSAANDWMGNAFKGWMPSGLYARALLI
                     MIVPMVILQSVVAFVFMERHWNTVTRHLSAAVVQDIASLIDVYNGYPQDKDRTQLRRI
                     AQQRLGLVVDFLAVGDMPPPGPKPFFSLLDQTLSVQLSRQIAKPFWIDTVGRSNLVEI
                     RIQLDDAVMRIFAQRSAAYASNWQIFIFWMLGTSSILLLVAVLFLRNQIKPILRLADA
                     AESFGKGREAPNFRPRGASEVRRASQAFLEMKARIERSIEQRTAMLAGVSHDLRTILT
                     RFKLELALIGDSPEVDGIRKDVDEMAGMLEDYLAFARGDSGEHAQPTDMTRALEELRS
                     DAERNG"
BASE COUNT          920 a         1647 c         1532 g          984 t
ORIGIN      
        1 cggcatattt gagctggttg cccatgttct tgggattgcc gagataaagc tcggcgcgga
       61 tttttgcggc acgcaaggcc gcgaccatct tctgataatc ggcgacgcga tcgcggtcga
      121 acacggtgac gacgacgggg ccgaactcgg gcttggtgtc gagcttgccc agcatcgtga
      181 gtgccgcctg cagccgcgac acgccgatgg aaaagccggt tgccggtacc ggctcgccgc
      241 gaaaccgcga gacgagacca tcatagcgcc cgccgccgcc gaccgagccg aaccgcacgg
      301 ggcggccttt ttcgtctttg gtctcgaggg taagttcgac ctcgtagacc gggccagtgt
      361 agtattcgag gccgcgcacg acggacgtgt ccataagtac gcgaccatcg ccataatccg
      421 atgccataat aatatcaaaa atctggctga gttcctttaa accttcatta ccagtcgtgc
      481 tgcccttctc attaaagaca ttttcaagtc tctgaaaaaa gcttttgtcg tttgcatccg
      541 agccaccgtg cataaatgca aaaacagtct cgatatctga catcgagatt ccggcacctt
      601 tcgtgaaatc gccagattcg tccttgcgac cttcgccgag caactcgcgc acgcccttct
      661 cgccgagcct atctagcttg tcgattgccc ttagcaccgt gagcctacgt cccgcattct
      721 catcgccacc gagcccgatg ctctccatca ccccatcaag caccttgcgg ctattcacct
      781 tcaccacata gctgccacgc ggaatgccca acgcttccat cgtgtccgcc gccatcatgc
      841 acatttcagc atcggccgcc ggcgacgcgc tgccgaccgt gtcggcgtcg aactgcatga
      901 actgacgaaa acggccgggc cccggcttct cgttgcgata gacgtagccg gcgcggtagc
      961 tgcgataggg ttttgggagg gcgtcaaaat tttccgcgac atagcgcgcc aagggtgcgg
     1021 tcaggtcgta gcgcaggctg atccattgct cgtcatcgtc ctggaacgag aacacgccct
     1081 cgttgggccg gtcctgatcc ggcaaaaatt tgccgagcgc gtcggtgaat tccatcgccg
     1141 gggtttccac cggctcaaaa ccgtagcgtt cgaacacctc gcggattttc tcgaccatag
     1201 cgcgggtcgc cgcgatctcg gcaggcccgc gatcggcaag cccgcgcggc agccgcgcgc
     1261 gcagtttctg gggttttttc ggtttctcgg ccatgggcgg cgttggtacc agcgcaggca
     1321 cgatgcggca acctcgcttg cttctccctc tcccgcaagc gggagaggga gcagatcagc
     1381 gcccagagag ctcgttcccc ggatgctgcg cagcgcgccg ctcttcgcgg cgtggtgcgc
     1441 tgcagatccg gggtccacga gccacaatcg gcgccgtcat gggtcccggc tctgcggagc
     1501 agcgcgaaga gcgctgcacc gcgtccggga cacgagaacc cctactacgc cgcggcggcc
     1561 ttgcccttgg gctgcacttc ggcggtgtag ccggccatca gctgctcgga aatcgcaccc
     1621 ggcttgaact tccactgcgc gatctccgac accggcgtga tctcggcggc ggtgccggtg
     1681 atgaagcatt cggaaaagcc cgaaagttcc tccggcatga tgcggcgctc gatcacctcg
     1741 atgccgcggc gtttggcgag gtcgatcgcg gtggcgcggg tgatgccggc gaggaagcag
     1801 tcggctgtgg gcgtgtgaat cttgccatcc ttgatgaaga agatgttggc gccggtgcat
     1861 tcggcgacgc ggccctgcca gtccagcatc atcgcgtcgg catagccttc ccgttccgcc
     1921 ttgtgcttac tgatggtgca gatcatgtag aggccggccg ccttcgccag cgcagggatg
     1981 gtcgcgggat ccggccttcg atattcggca agacctaacc ggatgccctt cagccgttcg
     2041 gcggggtcga aatagctcgg ccaggtccag caggcgatgg cgaggtggat ggtattggtc
     2101 tgcgccgaca cgcccatcat ttccgagccg cgccaggcga ccgggcggag ataggcctcg
     2161 ggcagattgt tcttttccag caccagccgc ttggcggcat caatctcggc gaccgaccag
     2221 ggaatctcga aatccaggat gcgggcggaa ttcttcagcc gctccgaatg ctcggtgccc
     2281 ttgaagatcg tcccgccata ggcgcgctcg ccctcgaaca cgcagctcgc ataatgcagc
     2341 ccgtgggtca gcacatggac gttggcgtcg ccccatggca ccagtttgcc atcgtaccag
     2401 atgacgccat cgatcttatc gaacgaaact cccatgggct ttctcccgga ttgacgctga
     2461 tcgcgcgccc gcgccgcggc gctgttggtt acgcctgggc cctcacctac accagccttg
     2521 cagccggttt ccatcctcac atgtccgtgg acatgctttt gtggtaggcg gaagtccagt
     2581 attgttgccg atataccgct cgacaatcgt acggtagata attcgcggct cgcgccgcca
     2641 cctttggcct gctccggccg tttgaaacag gactgccgac ggaacgaacg acttaatatt
     2701 tcgtcgcggc tgatagtttt gtaacattga acccaagata tgtcaatatg gctgacgtaa
     2761 atttatcgaa tccctcgtcc gaccctgatt cgcgggccac cgaggcgccg tccgctgccc
     2821 cgcggtccgg cgatttacgc tgggatatca tcgagctgtt gttcttcgcc tatcgggatt
     2881 tcgtcggtga cgccgatcat gagctggagg ccttcgggtt cgggcgggcg catcaccgcg
     2941 tcatgcattt cgtccaccgt tatcccggcc tgaaggtggc cgatttgctg gacgtactgc
     3001 gcatcaccaa gcaatcgctg ggacgggttc tcaagcaatt gctcgatgaa gggtatatcg
     3061 tgcagaagac cggcaacaac gaccggcggc aacgtcttct tttcgctacc gcaaagggag
     3121 aggcgctggt gggcaagctc gctggcctgc agaccgatcg catcaaccgc gcgctgcggg
     3181 acatcggtcc gtcggaggcg gaaattgtcc ggcagttcct gcgcgcgatg atcgaccacg
     3241 acgatcccga caaggtgctg gcgactatac ttggtgccgc caacgcaact gcaacggcaa
     3301 aggaataaac ccgtggccca agctgcaacc ctcgcccgaa agccgccgca accggccgac
     3361 gacgccccgc atgtgctgct ggtcgacgac gatcgccgca tccgcgacct gttgtcacgc
     3421 tttctctgcg gcgaaggcta ccgtgttacc acggcgacgg gcgcgcagga cgcccgcgcc
     3481 aagctgctcg gcctgcattt cgatctgttg atcctcgacg tcatgatgcc cggggagacc
     3541 gggttcgaac tcgcccgctt catccgcacc tcatcgtcgg tgccgatcat catgctgacg
     3601 gcgcgccatg aggccgaaaa ccggatcgag ggcctgcaga ttggcgccga cgattatgtc
     3661 gcaaaaccgt tcgagccgcg cgaactggtg ctgcgtatcg gcaatattct caagcgcgcc
     3721 gcgccgccgc cggtggagac gctggaacag gccgcgttcg ggccctatgt ctatcatctc
     3781 gatcgcggcg aactgcgcca gggcgacgag gtcattcatc tcaccgatcg cgaacgcgag
     3841 atgctgcgca ttctcgccat cgcacggggc gaaaccgtgc cgcgcagcgc gctcaccggc
     3901 gacggcaccg tcaacgagcg tgcggtggac gtgcagatca accgtctgcg gcgcaagatc
     3961 gagcgcgatc ccgccaatcc gctgttcctg caggcggtgc gcggcatcgg ctatcgcctg
     4021 gtcgcatcgc cttgagtcac ttctccaatg aaacgcgcct gatgagcacg ctcgacaccg
     4081 gcctcacgct gatccgaacc gcagccggcc gcgtctcggc ggccaatgac tggatgggca
     4141 acgcgttcaa gggctggatg ccctcgggac tctacgcccg cgcgctcctg atcatgatcg
     4201 tgccgatggt catcctgcag tcggtggtgg cgttcgtgtt catggagcgg cactggaaca
     4261 cggtgacgcg gcatttgtcc gccgccgtcg tgcaggacat cgccagcctg atcgacgttt
     4321 acaacggcta cccgcaggac aaggaccgca cgcagctgcg gcgcatcgcc cagcaacggc
     4381 tggggctggt ggtggatttc ctcgccgtcg gcgacatgcc gccgcccggt ccaaagccgt
     4441 ttttctcgct gctcgaccag acgctctcgg tgcagcttag ccggcagatc gccaaaccgt
     4501 tctggatcga taccgtcggc cgctccaatc tggtggaaat ccggattcag ctcgacgacg
     4561 ccgtcatgcg catcttcgcc cagcgcagtg ctgcctatgc ctcgaattgg cagatattca
     4621 tcttctggat gctcggcacc tcgtcgatcc tgctgctggt cgcggtgctg ttcctgcgca
     4681 accagatcaa gccgatcctg cgcctcgccg atgccgccga aagttttggt aaaggccgcg
     4741 aagcgccgaa ctttcgcccc cgcggggcca gcgaagtgcg ccgtgcctcg caggcgttcc
     4801 tcgagatgaa ggcacgcatc gagcgctcga tcgaacagcg caccgccatg ctggccggtg
     4861 tgtcgcacga cctgcgcacc attctgaccc gcttcaagct cgaactggcg ttgatcggcg
     4921 atagtccgga agtcgacggc atacgcaagg acgtcgacga gatggccggc atgctggagg
     4981 actatctcgc tttcgcgcgc ggcgacagcg gcgagcacgc ccagccgacc gacatgacgc
     5041 gggcgctgga ggaattgcgc agcgacgccg agcgcaacgg cca
//
LOCUS       DASWGM010000526         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1422194,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000526 DASWGM010000000
VERSION     DASWGM010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422194"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VGA15_15310"
     CDS             <1..1194
                     /locus_tag="VGA15_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX9589102.1"
                     /translation="EIRVRQGDRLRILVENKLSQATTVHWHGVRVPNAMDGVPFLTQK
                     PIPSGEKFVYEFEVPDAGTYWYHPHHHSSEQVGRGLSGALIVEEIEPLPVDRDVVWVL
                     GDWRLARDASIVSDFGNRMEVTMAGRIGNTVTINGRVPETISVRAGERLRLRLINAAA
                     ARIFGLEFRDLRPLIVALDGQPVEPREPPEGRVVLGPAMRADVLLDMSGKPGATATVV
                     DTFYRDLSYKLVDLAYSSEPPVRTRPLDPPARLRANTMPEPDLDNAEKHTAILEGGMM
                     SGRGMMMGMGGHGMGWSINGVAASGHNMAPMITLSRGHSCVLAIRNDTAWFHPIHLHG
                     HSFRVIARNGQPTRDRDWLDTVLIPPHEAADIAFVADNPGDWMFHCHILDHQDGGLMA
                     VIRVA"
     gene            1208..1915
                     /locus_tag="VGA15_15315"
     CDS             1208..1915
                     /locus_tag="VGA15_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9589103.1"
                     /translation="MNAKHYLVVSAALVISVAFTAARADGDPKHGAKIYGSCAACHSL
                     EPNLHLTGPSLDGLWGKKAASAENFARYSSALKEQGFLWDEVSLNAWLTDPKAFVPGT
                     YMTFRGIEDKKVRGDLIAFLKVAMVPGGAITAVAQGFVDAETARGQAPEPLATAAPQD
                     QVTGVRFCRDTFFVATADRKERPFWEMNLRLKIDTSANGPKVGQPVLVPGGMQGDRAS
                     IVFSDLEEIGRLVKKKC"
     gene            2015..>2503
                     /locus_tag="VGA15_15320"
     CDS             2015..>2503
                     /locus_tag="VGA15_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589104.1"
                     /translation="MKSNTEMTAKRNQRFGSQPLRTATSKGRISMPDFRSPKMRPTTI
                     RRRSFLATIAGLAGVLSLARRSYGAETGAGDAGRGNARLAGETAMRPSRCPAPASTID
                     LKTLGTDHQVASISSAGDIYRVTTVSGRTASFSEFDVRFKTDSSARGPAEGIPVLIPA
                     SIR"
BASE COUNT          503 a          748 c          770 g          482 t
ORIGIN      
        1 gagattcggg ttcgccaggg cgaccggttg cgaatcctgg ttgagaacaa gttatctcag
       61 gcaacaacgg tccattggca tggcgtgcgc gtgccgaatg cgatggacgg cgtgccgttt
      121 ctgacccaga agccgatccc gtcgggcgag aaatttgtct acgaattcga ggtgcccgat
      181 gccgggacct actggtatca cccgcaccat cacagctccg agcaagttgg cagaggtctg
      241 tcgggggcgt tgatcgtcga ggaaatcgaa cccctcccgg ttgatcgcga cgttgtttgg
      301 gtcctcggcg attggcgttt ggcgcgtgat gcgtcgatcg tgagcgactt cggcaatcgc
      361 atggaagtga cgatggccgg gcgcatcggc aacaccgtca cgatcaatgg ccgcgttccc
      421 gagacgattt ccgtccgcgc tggagagcga ttgcgcttgc gtctgatcaa cgcggcggcg
      481 gcgcggatct ttggactcga gttcagggat cttcggccgt tgattgttgc gctcgacggc
      541 cagccggtgg aaccgcgcga gcccccggaa ggacgcgtcg tgctgggacc cgcaatgcgc
      601 gcagacgtgc tgctcgatat gagcgggaag cccggcgcga ccgctacggt ggtcgataca
      661 ttctatcgtg acctctccta caagctggtg gatctcgcct atagcagcga gcctcccgtc
      721 cgaacgcggc cactcgatcc gccggcgcgc ctgcgggcca acaccatgcc agagccggac
      781 ctcgacaacg ccgaaaagca cacggcgatc ctggaaggcg gaatgatgag tggccgcggc
      841 atgatgatgg gcatgggcgg tcacgggatg gggtggtcga tcaacggcgt tgccgcgagt
      901 ggccataaca tggcgccaat gatcactctt tcacgcggcc attcctgcgt tctcgctatc
      961 cgcaacgata cggcctggtt tcatccgatc catctccacg gacattcgtt tcgggtgatt
     1021 gcgcgcaacg gccagccaac acgcgaccgc gattggctgg acaccgtcct tattcctcca
     1081 cacgaagctg ccgacatcgc ctttgtcgcc gacaacccgg gcgactggat gttccattgt
     1141 cacattctcg atcatcagga cggcgggctg atggccgtca tccgcgtagc ctgactggag
     1201 ctgatcaatg aacgcaaaac actatctggt cgtcagcgcc gcgctcgtca tttcggtggc
     1261 gttcactgcg gcccgcgctg atggcgatcc gaagcacggc gcaaaaatct acggttcgtg
     1321 cgcggcctgc cattctcttg agcccaattt gcacctgacc ggaccgagcc ttgacggcct
     1381 gtggggaaag aaggccgcct cggcagagaa cttcgcgagg tattcgagcg cactcaagga
     1441 gcagggtttc ctttgggacg aggtttcgct gaacgcgtgg ctcaccgacc ccaaggcttt
     1501 cgtacccggc acctacatga cctttcgcgg gatcgaggac aaaaaggtgc gcggcgatct
     1561 catcgccttt cttaaagtcg cgatggtgcc gggcggcgcc attaccgcgg tcgcgcaggg
     1621 atttgtcgat gccgaaaccg cccgcgggca ggcgccagaa ccgttggcga ccgccgctcc
     1681 tcaggatcag gtgacaggcg tccggttctg tcgcgacaca ttcttcgtgg ccacagcgga
     1741 tcgaaaggaa cgcccgttct gggagatgaa cctgcgcctg aaaatcgata ccagcgcgaa
     1801 tggtccaaag gtcggacagc cggtgctggt accgggcggc atgcaggggg accgagcgtc
     1861 aatcgtgttt tcggatctgg aagaaatcgg acgactggtc aagaaaaagt gctgaagccc
     1921 tcgtgcacag ggagactgat gattcgttcc aaggcgcaaa atgagcgccg cggtcgattg
     1981 aaacgagtga aaccaaggaa acgttcatcg acacatgaag agcaatactg aaatgacggc
     2041 caaacgcaat caacgctttg gctctcagcc acttcgcacc gcgactagca aaggaagaat
     2101 atcaatgcct gattttcgat cgcccaagat gcggccgact accattcgcc gccgttcgtt
     2161 tcttgccact atcgctggct tggcaggagt cttatctctc gctcgccgca gctacggcgc
     2221 cgaaacaggt gccggcgatg cagggcgcgg caatgcacgt ctggcgggag agaccgcgat
     2281 gcgcccgtct cgctgtccgg cgccagcgtc gaccatcgat ctgaaaacgc tcggaacaga
     2341 ccatcaagtc gcctcgatca gcagcgccgg cgatatctat cgcgtgacga cagtttccgg
     2401 aaggactgcc tcgttttcgg aatttgacgt gcgcttcaag actgattcca gcgctcgcgg
     2461 acccgccgaa ggcattccgg ttttgatccc cgcgagcata cga
//
LOCUS       DASWGM010000527         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2418409,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000527 DASWGM010000000
VERSION     DASWGM010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2418409"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VGA15_15325"
     CDS             <1..363
                     /locus_tag="VGA15_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589105.1"
                     /translation="IAAAMGVSLFAVMFFMLFLYRRIVMTGSYVTITGKAFRPRVADI
                     GPLRYVLLGVCLLYLFAAVGLPLVTLLYASLQKIAVAFPAAGNWTLENFRVAVTMNAV
                     RSALTNSSALVPRPSASC"
     gene            363..923
                     /locus_tag="VGA15_15330"
     CDS             363..923
                     /locus_tag="VGA15_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9589106.1"
                     /translation="MGLLTWLIYRSRLPGSGLIEYIVMFPQAVPRLVFAFGLMWAWVV
                     FPIPIYGTLWLLLIAYLTVFLPLGVRTIAGVILQIDKSLEECAQMCGAPWAFRVRTVT
                     IPLLVPGLVAAWLLLFIASVRELGASILLMGPHSKVITPSIVESWFGTSSELTAALAL
                     IQTLVVAIAMIVLVAVTRRVGAHPVD"
     gene            944..2074
                     /locus_tag="VGA15_15335"
     CDS             944..2074
                     /locus_tag="VGA15_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589107.1"
                     /translation="MQASPLGAPASSSPPTPHIEVDRLELYYGNVPAVRGVSFNVFPG
                     EQLTLLGPSGCGKTTTLRAIAGLEQPTAGEIRIDGVPVYSSERGIHIRAEKRGLSMVF
                     QSYAIWPHMTVFENVAYGLRVRRTGATEIAEKVHHALDMVQMRSFASRRAAQLSGGQQ
                     QRVALARAFVFSPSVLLFDEPLSNLDAKLRADMRIELRELQHRLGITSVYVTHDLEEA
                     LAMSDRIVVMRDGLIEQTGTPDEIYRLPNSAFVADFVGSANLIRGRHRSDLDVDGLFA
                     LETSTGHIVYGMAYGRTVGAELVVSVRTVHLRISTEPPATAQNVWHVPVEQTVFQGDF
                     TQVHVGWGTQHLIARCAAMEPLAPGGEVYISVDPRHVVVLGA"
     gene            2253..>2376
                     /locus_tag="VGA15_15340"
     CDS             2253..>2376
                     /locus_tag="VGA15_15340"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9589108.1"
                     /translation="MTGVGEARGKQAEKLGYLGLGMMGFPMTRRLINAGYDLTVW"
BASE COUNT          409 a          722 c          722 g          523 t
ORIGIN      
        1 atcgcggctg ccatgggcgt gtcgctgttc gccgtgatgt tcttcatgct gttcctgtac
       61 cggcgcatcg taatgaccgg cagttacgta accatcaccg gcaaggcgtt ccggccgcga
      121 gtggccgaca tcggcccgct acgctacgtg ttgctcggcg tctgcctgct ctatctgttc
      181 gccgcggtcg gcttgccact ggtgacgctg ctctatgcgt cgctgcagaa gattgctgtc
      241 gccttccccg ccgccggaaa ctggacgctt gaaaatttcc gcgtcgccgt gacaatgaat
      301 gccgtccgct cggcactcac caacagctcg gctttggtgc cgcgaccatc ggcgtcgtgc
      361 tgatgggact gctcacttgg ctaatctacc gctcgcggct gcctggtagc ggcttgatcg
      421 aatatatcgt gatgtttccg caggcggtgc cccggctggt gtttgcgttc ggcctgatgt
      481 gggcctgggt ggttttcccg attccgattt acggaacgct atggctgctg ctgatcgcct
      541 acctcacggt gtttttgccg ctcggtgtgc gcaccatcgc cggcgtcata ctccaaatcg
      601 acaagagtct agaggaatgc gcgcagatgt gcggcgcgcc ttgggcgttc cgggtgcgga
      661 cggtgacaat cccgctgctc gtcccgggtc tggtcgccgc ctggctgctg ttgtttatcg
      721 ccagcgtccg cgagcttggc gcttccatct tgctgatggg cccgcactcc aaagtgatca
      781 caccatcgat cgtcgaatca tggttcggca ccagttcgga actcaccgcg gcgttggcgc
      841 tgattcagac gctggtggtc gccatcgcga tgattgtgct tgttgccgtc acgcgccgcg
      901 tcggcgcgca ccccgtcgac taaggatgtc aggaaccgcc atcatgcaag cgtcaccgct
      961 gggagcgccg gcatcgtcct caccgccaac accgcacatc gaggtcgacc ggcttgaatt
     1021 gtattatggc aacgttccgg cggtgcgtgg cgttagtttc aacgtcttcc ccggcgaaca
     1081 actgacgctg cttgggccct ccggctgtgg caagaccacc acattgcgcg ccatcgctgg
     1141 gctcgagcag ccgaccgcgg gcgaaattcg catcgacggg gtcccggtct attcgtcaga
     1201 acgcggcatc cacatacgcg ccgagaaacg cggcctgtcg atggtattcc aatcctacgc
     1261 gatttggccg cacatgaccg tattcgaaaa cgtagcctat ggactacgcg tacggcgtac
     1321 tggggcgact gagatcgcgg aaaaagtgca tcacgcgctc gacatggtgc aaatgcgcag
     1381 tttcgcatca cgacgggcgg cgcagctgtc cggcggacag cagcagcggg tagcgctggc
     1441 gcgcgccttc gtgttctcgc cttcggttct attgttcgac gaacccctat cgaatctcga
     1501 tgccaaattg cgcgccgata tgcgtatcga actgcgcgag cttcagcatc gcctcggcat
     1561 cacctccgtc tatgtcaccc acgacctgga agaagcgctg gcgatgtcgg accgcatcgt
     1621 cgtgatgcgt gacggactga tcgagcagac cggcacgccc gacgaaatct accggctgcc
     1681 gaatagcgcc tttgtcgcgg atttcgtcgg ctcggctaat ctcattcgtg gccgccatcg
     1741 ctcggatctc gacgtggacg ggctgttcgc actcgaaaca tctaccggac acatcgtcta
     1801 cggcatggcc tatgggcgca ccgttggcgc cgaactggta gtctcggtgc gtaccgtgca
     1861 ccttcggatt tcgaccgagc cgccggccac agcgcaaaac gtgtggcacg tgccggttga
     1921 gcaaacggtc tttcaaggcg acttcactca agttcacgtc ggttggggca cgcagcacct
     1981 gatcgcccga tgtgccgcga tggagccatt ggcgccaggc ggggaagttt acattagcgt
     2041 tgacccgcgg catgtggtgg ttttgggtgc atagcgcgaa tctccttcac tgttcgtttc
     2101 agggattggt tagaattagc aacggtgact gattatcttt ctttggtgtc gctctctagc
     2161 ggcctaatct cggcgaggac caagcgagta tgtatcgcgg cttccacacc gcgcgcgtcg
     2221 ccgttctgga tgcagctaac gggagaaatg cgatgactgg ggttggcgaa gcgcgcggaa
     2281 agcaagccga aaagctcggc tatcttgggc tcgggatgat gggatttccg atgacgcggc
     2341 gcctaataaa cgccggctac gacctaaccg tctgga
//
LOCUS       DASWGM010000528         6067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1725690,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000528 DASWGM010000000
VERSION     DASWGM010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725690"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VGA15_15345"
     CDS             <1..120
                     /locus_tag="VGA15_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEX9589109.1"
                     /translation="WLNRCRRLAKDWENLNRKALAFLRLASIRLMLRKLCNPA"
     gene            137..571
                     /locus_tag="VGA15_15350"
                     /pseudo
     CDS             137..571
                     /locus_tag="VGA15_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            complement(587..1654)
                     /locus_tag="VGA15_15355"
     CDS             complement(587..1654)
                     /locus_tag="VGA15_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HEX9589110.1"
                     /translation="MTYTPPPRDPKAPPIRINLLSDTQTRPTPAMREAIARAEVGDEQ
                     IGDDPTVNVLCERVADLLGKQAAVFLPSGTMCNVAATLVHCRPGDEILAHETAHIIAR
                     EGGAHAALGGFQITPLEGADGKFSPDTFRAALHPRSRYQPPQTVVSVEQTANIGGGTI
                     WNKADLDDVVKIARSGGLATHMDGARLMNACVASGIAARDMTAGWDSAWIDFSKGLGA
                     PVGAVIAGSRDFIDKVWRWKQRLGGSMRQAGICAAACVFALDHHVDRLAEDHANARAL
                     ARGLSQIAGMEVQQPETNLVFFKPDGAGVTGEKMVAALRQRGVLLTMMDSRIRACTHL
                     DVTAGMIEETVGLVRDIARGA"
     gene            complement(1826..2443)
                     /gene="rpsD"
                     /locus_tag="VGA15_15360"
     CDS             complement(1826..2443)
                     /gene="rpsD"
                     /locus_tag="VGA15_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX9589111.1"
                     /translation="MTKRSEAKYKIDRRMGQNIWGRPKSPVNRREYGPGQHGQRRKGK
                     LSDFGVQLRAKQKLKGYYANISERQFHGIYVEAGRMKGDTGENLIGLLERRLDTVVYR
                     AKFVATMFAARQFINHGHVKVNGRRVNISSFKLKVGDVVEVKESSKQLTVVLEAGQLS
                     ERDVPDFLEVDHGKMTAKFARIPALSDVPFAVQMEPHLIVEFYSR"
     gene            complement(2785..3564)
                     /locus_tag="VGA15_15365"
     CDS             complement(2785..3564)
                     /locus_tag="VGA15_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9589112.1"
                     /translation="MRGTVRKISMPRRLVADLMHASIKIPFVSLRRPLNVRPLLEARA
                     LAVQPPGWAAIFVKAFSLVAREEPVLRTLYARWPWPSFYELPRSIAMVAIARVEDGQD
                     CVLPQKVPAADTVSLIEVDAQIRHAKDAPISEVPSFRKMLRVTRLPLPLRRFMWLVGL
                     NFGRQRANWCGSFGVTSVAAYGAGELHALSPGPFILSYGVVEPNQTIDVVIRWDHRIT
                     DAALIAKALTRLEQVLNTEIAAELRGARQQAEPKPVRAVAT"
     gene            complement(3969..4124)
                     /locus_tag="VGA15_15370"
     CDS             complement(3969..4124)
                     /locus_tag="VGA15_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589113.1"
                     /translation="MNAISTVLIHAIPEANTETHPLRTITLFCCLGLVASLCLAISGF
                     DLGAGLT"
     gene            complement(4347..5126)
                     /locus_tag="VGA15_15375"
     CDS             complement(4347..5126)
                     /locus_tag="VGA15_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9589114.1"
                     /translation="MSAAIPGPLNRLRRQWREGRPTFGAIATIPSIQTVQIMARSGFD
                     WIIVDLEHGPIDLGSAHGMIVATSGTPCVPLARIAANEPWLAKAPMDIGALGINFPMI
                     CSREDAEKAVRSVRYPPRGDRLWGPFHAPFRWGVSMPDYMATADDDMICMITIEHVEA
                     VNRIDEIMAVPGIDVAVIGPGDLATSIDKRGRVDDPEVLALMARAEAGILKSGVPIGG
                     VARTPQQANQMIERGYLALALGFDWSLFARGIAASFEGIIR"
     gene            complement(5217..6014)
                     /gene="murI"
                     /locus_tag="VGA15_15380"
     CDS             complement(5217..6014)
                     /gene="murI"
                     /locus_tag="VGA15_15380"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX9589115.1"
                     /translation="MASSPTILVFDSGLGGLTVLREVVKVRPDAHYIYVADDAFFPYG
                     HHGEDQIIARVVPMIGELIATHAPDLIVIACNTISTLVMSHLRDAYKVPFVGTVPAIK
                     PACANSITKRVSVLGTKGTVKREYTRALIRDFAQGCDVTLVGSADLASLAESALSGGE
                     ISDSEIFAEIAPCFVGDGASDPTRTDTVVLACTHYPLLMDRLVKLAPWPVDWVDPAPA
                     IARRVADLLKAPGGEADHTGAQMIFTSRRPHTLIPALMPFFGGRVPA"
BASE COUNT         1157 a         1838 c         1935 g         1137 t
ORIGIN      
        1 tggctcaatc gctgccgaag gctcgccaag gattgggaga atctcaaccg aaaggcgctc
       61 gcgttcttgc gcctcgcctc aatccgcctc atgctcagaa aactctgcaa tccggcgtga
      121 agtctccgga cggactctta gggatccgat gatgccccgc cgctgggaat ccggcggcgc
      181 cggcatggtc ggcaccatcg gcgactatgc gcgctttgcg cagatgctgc tgaacggcgg
      241 cacgttcgag ggcaggcgtt atctgaagcc tgagacgatc gccttgatga catcggatca
      301 tatcgggccg gagacgaaga tcgcccacga ttatttgtat tttccgggtg cgaccagcgg
      361 cttcggcctt ggctttgccg tgcgcacgtc cacgccgccg aatacgtcgt ggcctttggg
      421 cgaatatcgc tgggacggcg tcgccggtac gttctttttt atcgatccga aggacgatat
      481 gttcgtgatc ttcatggtgc agaccccgtc gcagcgcgga cggcttcaac tggagctgaa
      541 gacgctgatc tatcaggcgc tcaagaactg atcgcctcga gtccgcttag gcgccccgcg
      601 cgatatcgcg caccagtccg accgtctcct cgatcatccc tgcggtcacg tcgagatggg
      661 tgcaggcgcg gatccggctg tccatcatcg tgagcagcac gccgcgctga cgcaaggctg
      721 cgaccatttt ctcgccggtc acgccggcac catcaggttt aaagaaaacc aggttggttt
      781 ccggctgctg cacttccatg ccggcaatct gcgaaaggcc ccgcgccagc gcgcgggcgt
      841 tggcatgatc ttccgcaagg cggtcgacgt gatgatcgag cgcgaaaacg caggcggcgg
      901 cgcaaatgcc ggcctggcgc atcgagccgc caaggcgttg cttccagcgc cagaccttgt
      961 cgatgaaatc gcgcgagccg gcgatcacgg cgccgaccgg cgcgccgagc cctttcgaga
     1021 aatcgatcca ggccgaatcc caacccgcgg tcatgtcgcg cgcggcaata cctgaggcga
     1081 cgcaggcgtt catgagccgc gcgccgtcca tgtgggtggc gaggccgccg gatctggcga
     1141 ttttcacgac gtcatcgaga tccgctttgt tccagatcgt gccgccgccg atattggcgg
     1201 tctgttcgac gctgacgacg gtctgcggcg gctggtaacg cgagcgcggg tgaagggctg
     1261 cgcgaaaagt atcgggcgaa aattttccgt cggcgccctc tagcggcgtg atctgaaatc
     1321 cgccgagtgc ggcgtgggcg ccgccttcac gggcgatgat atgggcggtt tcgtgggcga
     1381 gaatttcgtc gccgggccgg caatgcacca gtgtcgcggc cacgttgcac atggtgcccg
     1441 acggcaggaa caccgcggcc tgcttgccga gcagatcggc cacccgctcg cacagcacat
     1501 tcacggtcgg atcgtcgccg atctgctcgt cgccgacttc ggcccgcgcg atcgcctcgc
     1561 gcatggccgg cgttgggcgg gtctgggtat ccgacaacag gttgatgcgg atcggcggtg
     1621 ctttcgggtc acgtggagga ggggtatagg tcatgatgag gtctcttcac tgacgtcgtc
     1681 atggctgggc tcgtcccggc catccacgtc ttgctgcttg tcttcgacca taaagacgcg
     1741 gatgcccggg acaagcccgg gcatgacgaa acaaaaaggc cccggcaaaa ccggggcctt
     1801 cgatctccgg atatgccgcc aaaaatcagc gcgaatagaa ttcgacgatc agatgcggct
     1861 ccatctgcac cgcgaacggc acgtcggaca aagcggggat gcgggcgaac ttcgccgtca
     1921 tcttgccgtg atcgacttcg aggaagtcgg gcacgtcccg ctccgagagc tggccggctt
     1981 ccagaacgac ggtgagctgc tttgaggatt ccttgacctc gaccacgtcg ccgaccttca
     2041 gcttgaagct cgagatgttg acacggcggc cgttcacctt gacgtggccg tgattgatga
     2101 actgccgcgc ggcaaacatg gtggccacga acttggcgcg atacaccacg gtgtcgaggc
     2161 ggcgctcgag caggccgatc aggttctcgc cggtgtcgcc cttcatccgg ccggcctcga
     2221 cataaatgcc gtgaaactgg cgctcgctga tgttggcgta gtagccttta agcttctgtt
     2281 tggcgcgcag ctgcacgccg aagtcggaga gttttccttt gcggcgctgg ccgtgctggc
     2341 cggggccgta ttcccggcgg ttcaccgggc tcttcggccg gccccagata ttctggccca
     2401 tccggcgatc gatcttgtat ttcgcctcac tgcgcttagt catcgcgtcc tcttggtaag
     2461 catagccatc gcggatgcga tggcgttctg tttcaagttg aggaaacgcg ccctcctgtg
     2521 cgccgggaat ttcccggagc cgacaggccc aacccttaaa gcagaaggga aagaccacgg
     2581 gtcgcgaaac gcaacgcggg ccgaaaaccg gcccgcgagc aggcgggttc tagggagaaa
     2641 tggcggttct gtcaatgaag ggccgtattt acggccgtcg aaaactgtca ttccggggcg
     2701 tcgcggcagc gacgaacccg gaatctcgag attccgggcc tgcgcctggc ggcgcatccc
     2761 ggaatgacgg cggagggtcg gatatcacgt cgcgacggcc cggaccggct tcggctccgc
     2821 ctgctgcctg gccccacgca attcagcggc gatttcggtg ttcagcacct gttccagccg
     2881 ggtcagggcc tttgcgatca gggcggcgtc ggtaatgcgg tgatcccagc ggatcacgac
     2941 atcgatggtc tggtttggtt cgaccacccc ataactgagg ataaacggac ctggactgag
     3001 cgcatgcagc tcgccggcgc cataggcggc aaccgaagtt actccaaaac ttccgcacca
     3061 gttggcgcgc tggcgtccga aattgagccc aaccagccac atgaagcggc gaagcggcag
     3121 cggcagcctt gttacccgca gcatcttgcg aaaagacggt acctcgctga tcggggcgtc
     3181 cttggcatga cggatctgcg catcgacttc gatcagcgat accgtgtccg ccgcagggac
     3241 tttttgcggc agaacgcaat cctgtccatc ttcgacgcgc gcgatcgcca ccatcgcaat
     3301 gctgcgtggt agttcataaa agctcggcca tggccatctg gcataaagcg tgcggaggac
     3361 cggttcctct cttgccacca gcgagaacgc cttgacgaaa atcgccgccc agccgggcgg
     3421 ctgcacggca agcgcgcggg cctccagcag tggacggacg ttgaggggcc gtcgcagcga
     3481 aacaaagggt attttgatcg acgcatgcat gaggtcggcg acgagacggc gcggcattga
     3541 gatttttcga accgttccgc gcatcgttcc actcaagttc ccgcaacaac cggctgagtc
     3601 ggatcacccc gtccggctcc tgatctagca cgaaccaacc aacctgactg cagatggttc
     3661 tgcctttatg gcgcgggcga ggcgagcggc cgggtctcgg ccgctgcaca aatcctgagc
     3721 actttcaggg tgttgtgatc gtgaaaggaa gcatcacgca cggcgcctgc cgggatggaa
     3781 ccgggatctg cgcgcggttg gttgcaggtc caaagatgcg ggtcgggcgt gccgccgatc
     3841 acggcttcgc cttccagcac gagggcttga aatgatcgcg accggaacca aaaagtcctg
     3901 atgacgttcg gttcgggtcg atcgtgcgtc gctcgacggt gacgctggat agggcgatcg
     3961 aaggcagctt aggtcaggcc tgcgcctaga tcgaagcctg aaattgcgag gcagagcgaa
     4021 gcgaccaggc ctaaacagca aaatagagtg atcgtcctga gcggatgggt ttcggtattg
     4081 gcttccggaa tcgcatggat aagaacagtc gaaatggcat tcatggcaag ctccatattt
     4141 caaataggcg cttgcattgg taataggtgt cggtttcaga aatggtttca caggcccgga
     4201 aaacggtttc aaaaacaccc gcgaattgtt ttttggttag ccgcccgtga aacaattgcc
     4261 gatgtgatgc ccgctaccgg tgtcattata cagcgatggg aggaaagagg gttcaccccg
     4321 gcttgcctct ccgggtaccg ctcgcgtcag cgtatgatgc cttcaaagct cgccgcgatg
     4381 ccgcgggcaa acagcgacca gtcgaagccg agcgccagcg ccagatagcc ccgttcgatc
     4441 atctggttgg cctgttgcgg cgtgcgcgcc acgccgccga tcggaacgcc gctcttgagg
     4501 atgccggcct cggcgcgcgc catcagcgcc agcacttcgg ggtcatcgac gcggccgcgt
     4561 ttgtcgatac tggtcgcaag gtcgccgggt ccgatcaccg cgacgtcgat gccgggcacg
     4621 gccatgatct cgtcgatgcg gttgaccgcc tcgacgtgct cgatggtgat catgcagatc
     4681 atgtcatcgt ccgcggtcgc catatagtcg ggcatcgaaa cgccccagcg aaacggcgca
     4741 tggaacggtc cccagagccg gtcgccgcgc ggcggatagc gcacgctgcg cacggctttc
     4801 tcggcgtcct cgcggctgca gatcatcgga aaattgatgc caagtgcgcc gatatccatc
     4861 ggcgctttcg caagccaggg ctcgttggcg gcaatccgcg ccagcggcac gcaaggcgtg
     4921 cctgaggtgg cgacgatcat gccgtgcgcg gagccgaggt cgatcggacc gtgctcgagg
     4981 tcgacgatga tccagtcgaa tcccgagcgg gccatgatct gcacggtctg aatgctggga
     5041 atggttgcaa tcgcgccgaa ggtgggacgc ccctcacgcc attgcctgcg aagacggttg
     5101 aggggaccag gtatcgcggc ggaaatcaaa tccgaaactc cgttcgggtg gctcgggaag
     5161 cgcgtcgcct gaaaaaacta acagcgcggc aggatgtcga aaagatcaat atcgattcag
     5221 gcaggaacac ggccgccgaa aaacggcatt aacgccggga tcagcgtgtg cggccgcctg
     5281 gaggtgaaaa tcatttgtgc accggtgtga tcggcttcgc cgccgggggc tttaagcaaa
     5341 tccgccaccc gccgggcgat cgcgggcgcg ggatcgaccc agtccaccgg ccacggcgcg
     5401 agcttgacca gccggtccat cagcagcggg tagtgggtgc aggccagcac gacggtgtcg
     5461 gtgcgggtcg gatcgcttgc gccgtccccg acaaaacacg gcgcgatttc ggcaaatatc
     5521 tcggagtcgc ttatttcgcc gccgctcagc gcggattcgg ccagcgaggc cagatccgcc
     5581 gagcccacca gcgtcacgtc gcagccttgc gcgaaatcgc ggatcagcgc cctggtgtat
     5641 tcgcgcttga cggtgccctt ggtgccgagc accgacacgc gctttgttat ggagttcgcg
     5701 caggccggct tgatcgccgg caccgttccg acaaagggga ccttgtaggc atcacgaaga
     5761 tgcgacatca ccagggtcga gatcgtgttg caggcgatca cgatcagatc gggggcatgg
     5821 gtggcgatca attcgccgat cattggcacc acccgtgcga tgatctggtc ctcgccatga
     5881 tggccgtagg gaaagaacgc gtcgtccgcg acataaatgt aatgcgcatc aggccgtacc
     5941 ttgaccacct cacgcaatac cgtgaggccg ccaaggccgg aatcgaacac gagaatcgtc
     6001 ggagatgatg ccacgccgcg atcctagccg atccggggtt accgttcagt tgtttttggc
     6061 ggcacat
//
LOCUS       DASWGM010000529         7597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_175045,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000529 DASWGM010000000
VERSION     DASWGM010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175045"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VGA15_15385"
     CDS             complement(<1..423)
                     /locus_tag="VGA15_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9589116.1"
                     /translation="MSDLENFRRETRAWLEANCPPEMRRPMTSDEDTFWGGRNTKFSS
                     EPQRVWFERMRDKGWTVPHWPKEYGGGGLDPEQTKIVRQEMAALGARQPLVSFGISML
                     GPALLNYGTDAQKKEHLPKISAGLIRWCQGYSEPNAGSD"
     gene            578..1123
                     /locus_tag="VGA15_15390"
     CDS             578..1123
                     /locus_tag="VGA15_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9589117.1"
                     /translation="MRLKLLSPGEMSEDQKQTYDESIAGKRGKPPPPMMAWLNSPEMA
                     RHATRLGGFLRFDTLFPAKLSEIAILVTARHWTSHYEWYAHKRLALKAGTDPKIIEDI
                     RDRRTPKFDDPKGQMIYDVSKSLHEGHGVSKGLYDEAVKVLSERGLVEIIGLCGYYTM
                     VSMTLNTFEFDLPEGEVSELA"
     gene            1318..1815
                     /locus_tag="VGA15_15395"
     CDS             1318..1815
                     /locus_tag="VGA15_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9589118.1"
                     /translation="MPENSTPIIAGTRIGHVHLKVADLDRALGFYCGVLGFELTQRLS
                     SGAAFISAGGYHHHIGLNTWESKGGHPPAPGTTGLFHTAILYPARPALADALHRVMQA
                     GIHLDGASDHGVSEALYLRDPDENGVELYWDRPREQWPRKPDGSLAMFTHRLDLDDLL
                     RQREA"
     gene            complement(1825..3075)
                     /locus_tag="VGA15_15400"
     CDS             complement(1825..3075)
                     /locus_tag="VGA15_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9589119.1"
                     /translation="MLDSLSEQSIDETSARYDGWRIVAVCFLVATFGWGFGFYGQSVY
                     VAELHRLHGWPASLISGGTTFFYLFGALLVASVSEAIRGFGPRNCLVAGVLALATAVV
                     LIGQVTEPWQLYAADAVLAFGWAGTSLGIITNTLGLWFDKRRGMAISLALNGASFGGI
                     VGVPLLATVIGSLGFADAMIAAPIAMVVLMIPIILIFVGRPPIGSSAAIAAVSADAPS
                     ATQIRSKAFRDAAFLTVSIAFALVLFAQVGFIVHLISFLDPVIGRERAAIAVALLTAM
                     AMAGRVLFSTVIDRLNQRLASAISFVSQAVALSIIINSRNDVVLIGCCALFGFSVGNL
                     ITLPSLIVQREFDPRSFGVLISLITAINQVTYAFGPGIVGLLRDASGSYSLPFYGCIG
                     LELIAAVLIMACRGRGRAGTSATQ"
     gene            complement(3204..3566)
                     /locus_tag="VGA15_15405"
     CDS             complement(3204..3566)
                     /locus_tag="VGA15_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9589120.1"
                     /translation="MPSRARLDEFIAVVETGDHAGAIERYYTEDASMQENTLPPRVGR
                     DALVARERGVLAQMSHVYSKAMSSVVEGDHVAIHWIFELTDKSGKVRRVDEVALQEWR
                     GDRIFRERFFYDPSGPKT"
     gene            complement(3609..4853)
                     /locus_tag="VGA15_15410"
     CDS             complement(3609..4853)
                     /locus_tag="VGA15_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9589121.1"
                     /translation="MIDMTAADEISGDARARSNVMRLAAAQALTGANSAVIFATGSIV
                     GATLAPSISFATVPLSMYVVGLAAGTLPTGAISRAYGRRVAFIIGTGCGMLTGLLGAF
                     AILRGSFVLFCCATLLGGLYGAVSQSYRFAAADGASAEFRAKAVSWVMAGGVFAGVLG
                     PQLVQWTMDIWPPYLFAFSFVMQAMVALIAMAVLAGVDAPKPAPSDLHGGRPLLEIVR
                     QPRFIAAAFCGIVSYPMMNLVMTSAPLAMKMCGLSVSDSNFGIQWHIVAMYGPSFFTG
                     TLIARFGAPVIVALGLSLEAAGAMIGLSGITALHFWATLMVLGLGWNFSFVGASALVL
                     ETHRPQERNKVQAFNDFLVFGMMAVGSFSSGQLLASYGWSAVNMVVFPPVLLGLAVLA
                     VASFARRRAAPQQVAEFPDPGI"
     gene            5069..7145
                     /locus_tag="VGA15_15415"
                     /pseudo
     CDS             5069..7145
                     /locus_tag="VGA15_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
     gene            complement(7202..>7597)
                     /locus_tag="VGA15_15420"
     CDS             complement(7202..>7597)
                     /locus_tag="VGA15_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX9589122.1"
                     /translation="RPPLRIDLTDPMWCPQMQDGSGPGELLAVFCANVIHIAPWRVAE
                     GLFAGAGRYLRADGRLFLYGPFKRGGKHTAVSNAVFDSSLREGNPEWGVRDIEALQEL
                     AATAGLALVDVAEMPANNLILVFERKRAD"
BASE COUNT         1586 a         2393 c         2343 g         1275 t
ORIGIN      
        1 gtccgatccg gcgttgggct cggaatagcc ctggcaccag cgaatgagtc cggctgatat
       61 cttcggcaga tgttccttct tctgcgcgtc ggtgccgtag ttcaacagtg cgggcccgag
      121 catggaaatg ccgaacgaga caagcggctg gcgcgcgccg agcgccgcca tctcctggcg
      181 gacgattttt gtctgttccg gatcgaggcc tccgccgcca tattccttcg gccaatgtgg
      241 cacggtccag cccttgtcgc gcatccgctc gaaccagacg cgctgcggct cggaggagaa
      301 tttcgtgttg cgaccgcccc agaaggtatc ttcgtccgag gtcatcgggc gccgcatctc
      361 cggcggacaa ttggcttcca gccaggcgcg ggtttcacgg cgaaaatttt ccaggtcgga
      421 catgggcgtt tcctcaactg ttggatcgac cttagccatc gcatcgcgga attcaatata
      481 tctctggcgc ggcccatgcg gcgacggatg tggtcatacc ccccgatccg gtgtattcgg
      541 caaagccgca acgattgaaa aacaagagga aaccgacatg cgcctgaagt tgctttcgcc
      601 tggcgaaatg agcgaggacc agaaacaaac ctatgatgaa tcgatcgccg gcaagcgcgg
      661 caagccgccg ccgccgatga tggcctggct caacagcccg gagatggcgc ggcacgccac
      721 gcgcctgggc ggattcctgc ggttcgatac gctatttccg gcgaaattgt ccgagatcgc
      781 gatcctcgtc accgcgcggc actggacctc gcattacgaa tggtatgccc ataagcggct
      841 cgcgctgaaa gccggcacgg atccgaaaat catcgaagac atccgggacc gccgcacgcc
      901 caagttcgac gatcccaagg gccagatgat ctacgacgtc agtaaatcgc tgcacgaagg
      961 ccacggcgtt tccaagggcc tgtacgacga ggccgtaaaa gtgcttagcg agcgtgggct
     1021 ggtcgaaatc atcggcctgt gcggctatta caccatggtg tcgatgacgt tgaatacttt
     1081 tgagttcgat ctgccggagg gagaagtgtc ggagctcgcg taagaggaac tccctcgccc
     1141 cgctcttgcg gggtcgagac gagcgaagct cgctcttaga gggttggggt gaggggctct
     1201 ttcggaaaat tcctgagcta atgatagact agtaccccct cacccggatc gcctctccga
     1261 ggcgatccga cctctcccgc aggcggggcg aggtgaagag tcaacggaga aatgcacatg
     1321 cccgaaaatt caacgcccat catcgcggga acgcggatcg ggcatgttca tctcaaggtg
     1381 gccgatctcg atcgcgcgct tgggttctat tgcggcgtgc tggggtttga gctgacgcag
     1441 cgcctgagtt cgggtgctgc gttcatatcg gccggcggct atcaccacca catcggcctg
     1501 aatacctggg aaagcaaagg cggccatccg ccagcgccgg gaacgacggg gctgtttcac
     1561 accgcgatcc tttatccggc ccggccggcg ctcgcggacg cactgcatcg cgtgatgcaa
     1621 gcgggaattc atctcgacgg cgccagcgat cacggcgtca gcgaggcgct ctacctgcgc
     1681 gatcccgacg agaacggcgt cgagctgtat tgggaccggc cgagggaaca atggccgcgc
     1741 aagccggacg gatcgctggc gatgttcacg catcggctcg atctcgacga tttgctgcgc
     1801 cagcgggagg cgtgaccctt atcgtcattg cgtggccgac gttcctgccc gcccgcgccc
     1861 tctgcaagcc atgatcagga cggcggcgat cagctcaagc ccgatgcagc cgtaaaacgg
     1921 cagcgaatag cttccggacg catcgcgcag caggccgacg atccccggac cgaacgcata
     1981 ggtcacttgg ttgattgccg tgatcagact gatcagcacg ccgaacgagc gcgggtcgaa
     2041 ttcgcgctgc acgatcagcg acggcagcgt aatcagattg ccgaccgaaa atccgaacag
     2101 cgcgcaacac ccgatcagca ccacatcgtt gcgcgaattg atgatgatcg atagcgccac
     2161 ggcctgactg acaaacgaga tcgccgacgc cagccgctgg ttgagccggt cgatcacggt
     2221 tgagaacagc acgcgcccgg ccatcgccat cgcggtcaat agcgccaccg cgatcgcggc
     2281 gcgctctcgt ccgatcacgg gatcgaggaa cgaaatcagg tggacgatga agccgacctg
     2341 cgcaaacagc accagcgcga atgcaatgga tacggtcagg aacgccgcgt cgcgaaatgc
     2401 cttgctgcgg atctgcgtgg cggacggcgc atccgcactg accgccgcga tcgcggcact
     2461 cgaaccgatc ggcggccgtc cgacgaaaat cagaatgatg gggatcatca gcacgaccat
     2521 ggcaattggc gctgcgatca tggcgtcggc aaatccaaga ctcccgatca cggttgccag
     2581 caggggaacg ccgacgatgc cgccaaaact tgccccattc agcgcaagac tgatcgccat
     2641 gccgcgcctc ttgtcgaacc agaggccaag cgtgttggtg ataatcccga gactggtccc
     2701 ggcccagccg aaggcgagca cggcatcggc ggcgtacagc tgccacggct cggtgacctg
     2761 accgatcagg acgaccgccg tggcaagcgc caacacgccc gccacgaggc aattgcgcgg
     2821 gccgaagccg cggatcgctt cgctgacgga ggcgaccagc aacgcgccga acaaatagaa
     2881 gaacgtcgtg ccgcctgaga tcagcgaggc cggccaaccg tgcaggcgat gcagctcggc
     2941 gacatagacg ctctggccat agaagccgaa cccccagccg aacgtcgcca ccagaaagca
     3001 gaccgcgacg atccgccagc cgtcgtatcg ggcggaggtc tcgtcgatgg attgttcact
     3061 gaggctatcc aacatttatc gctttggctt ttttcaccgt catggccggg cttgtcccgg
     3121 ccatgacggt tcaattccaa aacttaacag agcagcaacc gcaacgcttc gatcaccgtc
     3181 gaagtgtcaa tcaggcgggc cggtcaggtc ttcggcccgg acggatcata gaagaaacgc
     3241 tcgcgaaaaa tcctgtcgcc gcgccattcc tgcaatgcga cctcgtctac ccggcgcacc
     3301 ttgccggatt tatcggtcag ttcgaagatc cagtgaatgg cgacatgatc gccctcgacc
     3361 accgaggaca tcgccttgga atagacgtgc gacatctgcg ccagaacgcc gcgctcgcgg
     3421 gccaccagcg cgtcgcggcc gacgcgcggc ggcaaggtgt tctcctgcat gctggcatct
     3481 tcggtgtaat agcgctcgat cgcgccggca tgatcaccgg tctcaacgac cgcgataaac
     3541 tcatcgagac gcgcgcgcga tggcatcaaa cctccaacgt gtcaggcaat cattgcaagc
     3601 atatcagatc aaatgcccgg atcgggaaat tccgcaacct gctgcggcgc ggcgcgccgc
     3661 cttgcaaacg acgccaccgc cagcaccgcg agccccaaca gcaccggcgg aaacaccacc
     3721 atattgaccg ccgaccagcc gtaactcgcc agcaactggc ccgacgagaa cgagcccacc
     3781 gccatcatcc cgaacaccag aaaatcgttg aaggcctgca ccttgttgcg ctcctgcggc
     3841 cggtgcgtct ccagcaccag cgcggaagca ccgacaaagc tgaaattcca ccccagcccg
     3901 agcaccatga gcgtggccca gaaatgcaag gcggtgatgc cggagagccc gatcatcgcg
     3961 cctgcggcct cgagcgacag accgagcgcg acaatgacgg gcgcgccgaa gcgcgcgatc
     4021 agcgtgccgg taaagaagct cggcccgtac atcgccacga tatgccactg aatgccgaaa
     4081 ttcgaatcgc tcacgctcag gccgcacatt ttcatcgcca gcggcgcgga ggtcatcacc
     4141 agattcatca tgggatagga cacgataccg caaaacgcgg ccgcgatgaa ccgcggctgc
     4201 cgcacgatct cgagcagcgg acggccgccg tgaagatccg atggcgcagg ttttggggcg
     4261 tcgacgccgg ccagtacggc catcgcgatc agagcaacca tcgcctgcat cacgaagctg
     4321 aaggcaaaca ggtatggcgg ccagatatcc atcgtccact gcacgagctg cgggccgagc
     4381 acgccggcga acacgccgcc ggccatcacc cacgacaccg ccttcgcacg aaattccgcg
     4441 ctggcgccgt cggccgcggc gaaacgatag gattgcgaga ccgcgccata aaggccgccg
     4501 agcaaggtcg cgcagcaaaa cagcacaaac gaaccgcgca ggatcgcgaa tgccccgagc
     4561 aggccggtca gcatgccgca accggtgccg atgatgaagg cgacgcggcg accataagcg
     4621 cgcgaaatgg cgccggtcgg cagcgtgccg gcggcgagtc caaccacata catcgacagc
     4681 ggtaccgtcg cgaacgagat cgacggcgcc aaggtcgcgc cgacgatcga gccggtggcg
     4741 aaaatgaccg ccgagtttgc gccggtcagc gcttgtgccg ccgcgaggcg catcacattg
     4801 gaccgcgccc gggcgtcgcc ggaaatctcg tccgctgcgg tcatatcgat catcggctat
     4861 ttccaccctg gcgcggccaa atgcacccgg ccttattgat atcaggtact atgaagccag
     4921 gtttgcaccc aatcaaccga tgcaaacggg tgtgcgccat gcgggcgatg cacattggac
     4981 aggagacgcg ccgccgtcta aatcagaagc tgccgtttat gttgcgagcc gtgcggaatg
     5041 cgcgcccagc acccgtggaa ttcgaaccat gcccatcgac gacaggccga ccattgccgc
     5101 ccccgacgac gatccctatc tgtggctgga agaaatcgag ggtgaacgtg cactggattt
     5161 cgtcgagcag cagagcaagc ggacgctgga gagattcggc aatgccggat ttgctgacga
     5221 tcgtgacacg ctggctgcga tttacgaccg gccggacaat attccctacg tcggccggcg
     5281 cggcgggttg ctctacaatt tctggaagga tgcgaataat ccgcgcggca tctggcggcg
     5341 aacgacactc gaggaatttc gcgggccaaa cccgaaatgg gacatcctcc tcgacctcga
     5401 cagacttgcc gccgaggaag ccgaagactg gatcctgggc ggggtggagg cactgccaga
     5461 cagtaatgct cgaattctaa acctttcacg cggtggcggc gacgccaccg tgaagcggga
     5521 attcgatatc ggcaccagaa cattcgtacc cgatggtttc acgctgccgg aagccaaggg
     5581 gggccgtgaa gggcgccgaa tggctcgacg ccgacaccgt cttgctgtcg agcgcgtatg
     5641 gcgagggcat gacgaccacg tcgggctacg ccaggacggt gaggctatgg cggcgcggca
     5701 cggacgtgaa tcgggcgccg gtgatcttcg agaccgcgac cgataacatt tcggcgttct
     5761 caagcatcga ccgcaccggc gccagcctga gggtgtggtt cgtcgaatgg cttgatttct
     5821 tcaattatcg tctttggctc ggcgacgagt ccggcgcaaa gacaaggctc gatctgccaa
     5881 ccgacatctg ggcgcaagcc tatcaggatt ggcaggtggt gaaattgcgg acggcttggt
     5941 cagcgggtgg aaggagcttt gcgccggata cggtgctcgg catctcgctg tcggcctttc
     6001 tggccgggga ccgcaatttc gaagttattt ttgagccggg accgcgccgc gccctgcagg
     6061 gctttttctg gaatgcgggc aggctggtgc tgcccattct cgacgaattg cgaccggttt
     6121 tcgaggtttg gacgccatcg gcaaatggct ggacccattc caacttgcag ggattgcccg
     6181 agatcggcgt cgtcgatgtc tggcctctcg acgctgacat atccgaaagc aacggcgacc
     6241 tgcttgcgaa tgtccaggac ccgctgacgc caccctcctt gatgctgata gaaggggtcg
     6301 gaagcccggc gctgttgaag caggccccgc ggacgttctc cgcagacggg ctcgtgatca
     6361 cgcagcatga ggccatctcg gtcgacggcg agcgcattcc ctatgtgcag accggacccg
     6421 ctgacgggac cggcgatgca ccggtgcatc tcagcgctta tggcggcttt ggcattgcga
     6481 tgcggcccta ttacaattcg gcaatcggca aactttggct tgaacgcggc ggcaccagtg
     6541 tcgtcgccaa cattcgcggc ggcggcgaat tcggtacacg ctggcatgac gccggccgat
     6601 atgccggcaa gaggctcgcg catgatgatt ttgccgccgt cgccgccgac ctcgtcaggc
     6661 gcagcgtgac gtgcgccaac cggatcgccg cggaaggcgg atcgaacggc ggcattctga
     6721 tctccaacat gctgacgcgc tatcccgaac gttttggagc gttgttctgc acgattccgc
     6781 tgatcgacat gcgccgctac accaagctgc tggcaggtgc gagctggatc gcggaatacg
     6841 gcgatcccga caaacccgaa gaatgggcct ggctgcagac ctattccgcc tatcatgccg
     6901 caaaacccgg acagacatat ccgccgatcc tgatcgccac cacgcggcgc gatgaccgcg
     6961 tgcacccggg acacgcgcgc aagatgaccg ccaagctaca ggcgatggga tatgaagcgt
     7021 atctttacga gcccgcggcc ggcggtcacg gctatggcaa ggacaacagg gaacgcgccg
     7081 cattcacggc gcttggatat gcgttcctga agaacaagat cggctggtca gaccatcaga
     7141 tgtaaagcgc gcgtcctgtt tggtggaagt tgggtttcgc gcacgcgcga cttgcgaacg
     7201 ttcaatcggc tcgcttgcgt tcgaaaacca gaatcagatt gttggcgggc atctcggcga
     7261 cgtccacaag cgccagaccg gcggtcgccg ccagttcttg cagggcctcg atatcgcgca
     7321 cgccccattc gggattgcct tcgcgaaggc tcgaatcgaa cacggcgttg ctgaccgcgg
     7381 tgtgcttgcc gccgcgcttg aacggcccgt agaggaacag ccgcccgtcc gcccgcaaat
     7441 aacgtccggc gcctgcgaac agaccctcgg cgacgcgcca cggcgcgatg tggatcacat
     7501 tggcgcagaa caccgcgagc agttcgccgg ggccgctgcc gtcctgcatc tgcgggcacc
     7561 acatgggatc ggtgaggtcg atccgcagcg gcgggcg
//
LOCUS       DASWGM010000530         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2137728,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000530 DASWGM010000000
VERSION     DASWGM010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2137728"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VGA15_15425"
     CDS             complement(<1..265)
                     /locus_tag="VGA15_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheR"
                     /protein_id="HEX9589123.1"
                     /translation="MTPLDYEFLRKLLKERSGLDLSSDKQYLVESRLIPLARRIGLAG
                     IAELVAKMKGGSDALTAEVAEAMTTNETFFFRDKIPFDHLRETV"
     gene            complement(262..1431)
                     /locus_tag="VGA15_15430"
     CDS             complement(262..1431)
                     /locus_tag="VGA15_15430"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEX9589124.1"
                     /translation="MTVALTIAPPPNSTRQEPLRVMVVDDSAVIRGLISRWVSSEPDM
                     VVAASLRTGLDAVNQIDRVNPDVALLDIEMPELDGISALPLLLAKKRNLIIIMASTLT
                     RRNAEVSFKALSLGASDYIPKPESTREPAAAETFRHDLIQKIRHLGAKVRRAVPASPS
                     PSAVPVPGRARELSPRPALAPVVSPPIIRRAFSAQAPRALLIGSSTGGPQALMTLVTE
                     LGAVIDRFPVLITQHMPPTFTTILAEHLARSSHRPAREAVDGEIVKAGQIYLAPGGRH
                     MRVARDGTDIVIALDDGPPVNFCKPAVDPLFTSAINVWQGGVLAVVLTGMGSDGMRGG
                     KEIVAAGGSVIAQDEASSVVWGMPGAAAHAGICAAILPLNQIAPKLARVFSGDRS"
     gene            complement(1509..1874)
                     /locus_tag="VGA15_15435"
     CDS             complement(1509..1874)
                     /locus_tag="VGA15_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9589125.1"
                     /translation="MRTCLVVDDSSVIRKVARRILEGLDFQIIEAEDGEKALEICKRG
                     LPEAILLDWNMPVMDGYEFLGNLRRMPGGDQPKVVFCTTENDVAHIARALHAGANEYI
                     MKPFDKDIVTAKFQEVGLL"
     gene            complement(1945..>2130)
                     /locus_tag="VGA15_15440"
     CDS             complement(1945..>2130)
                     /locus_tag="VGA15_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9589126.1"
                     /translation="LLIDQIGEVLKLADDGREENPVNLDPRMTKLAGGVHRLDGQLMV
                     VLDVDRVLEIVPNMLAA"
BASE COUNT          426 a          653 c          645 g          406 t
ORIGIN      
        1 gcaccgtttc ccgcagatga tcgaacggaa tcttgtcgcg aaaaaagaag gtttcattgg
       61 tggtcattgc ctcggccacc tccgccgtca gcgcgtcgga tccacccttc attttcgcaa
      121 cgagttcggc tattccggcc aaaccgatcc ttcgcgccag cgggatcaac cggctttcca
      181 ccagatattg cttgtcggaa gacaggtcca gtccggagcg ctctttcagg agcttacgca
      241 aaaactcata gtccagcggc gtcacgaacg atctcccgaa aacacccggg ccaatttcgg
      301 agcgatctga ttgagcggca gaatggccgc gcaaatgccc gcatgcgcag ctgcgcccgg
      361 catgccccaa accacgctcg aagcttcgtc ctgggcgatc acgcttccgc cggccgcgac
      421 gatttccttg ccgccgcgca tcccgtcaga gcccatgccg gtcagcacga cagccagaac
      481 gccgccctgc cagacattga ttgcggaggt gaacaacggg tcgacggcgg gcttgcagaa
      541 attgaccggc ggcccatcgt cgagggcgat cacgatatcc gtaccatccc gcgcgacgcg
      601 catatgacgg ccgcccggcg caagatagat ctggccggcc ttgacgattt ccccgtcgac
      661 cgcttcgcgc gccggccgat ggcttgagcg cgcgaggtgc tcagcaagaa tcgtcgtgaa
      721 tgtgggcggc atatgctggg tgatgagtac cggaaaacga tcgatcacag ctccgagttc
      781 ggtgaccagc gtcatcagcg cctgtggacc tccggtcgat gaaccgatca ggagcgcgcg
      841 cggcgcttgc gcgctgaacg cgcggcggat tatcggtggc gagacaaccg gagccagcgc
      901 cggccggggc gatagctcac gcgctcgacc cggcaccggt accgcggaag gagacggtga
      961 ggccgggacc gcgcggcgga ccttggcgcc gagatggcga attttctgaa tgagatcgtg
     1021 gcgaaaggtt tcggcggcgg cgggttcccg ggtgctttcc ggctttggaa tatagtctga
     1081 tgcaccaagc gacagcgcct tgaagctgac ctccgcgttg cggcgggtca gggtcgaagc
     1141 catgatgatg atgagattgc gtttcttcgc aagcagcaag ggcagcgccg aaatgccgtc
     1201 gagctccggc atttcgatat cgagcaaggc aacatcggga ttgacccgat cgatctgatt
     1261 gaccgcgtca agacctgtgc gcagcgacgc cgcgacaacc atgtccggct cggatgagac
     1321 ccagcgggag atcaaccctc tgatgaccgc ggagtcgtcg acgaccatca cccgcaacgg
     1381 ctcttgccgc gtcgaattcg ggggtggggc aattgtcaac gcaacagtca ttattcaccc
     1441 gatacggctg aaagcggcac tataaaaacg ataacgccga aggccgttca gccgccggat
     1501 cgaacggatc agagcaggcc gacttcctgg aatttcgccg tgacgatgtc cttgtcgaat
     1561 ggcttcatga tgtattcgtt ggcaccagcg tgaagcgcgc gcgcgatgtg cgcgacatcg
     1621 ttttcggtgg tgcagaacac caccttgggc tggtcaccgc cgggcatgcg gcgcagattg
     1681 ccaaggaatt catagccgtc catcaccggc atgttccagt cgagcaggat cgcctcgggc
     1741 aatccgcgct tgcaaatctc aagcgccttc tcgccgtcct cggcctcgat gatctggaaa
     1801 tccagacctt ccaggatgcg ccttgcgacc tttcgaatga cgcttgagtc atcgaccacc
     1861 agacatgttc tcattttagc ctctgcttct ttcagatccc gagggacgtt cccattgcag
     1921 cgtatgctgt gcctgttact gatctcatgc cgccagcatg ttaggcacga tttcgaggac
     1981 gcgatcgaca tcgaggacga ccatcagctg gccgtcgagg cgatgcacgc cgccggcgag
     2041 cttggtcatg cggggatcga ggttgacggg attttcctcg cggccgtcat cggcgagctt
     2101 gagcacctcg ccgatctgat cgatcagcag
//
LOCUS       DASWGM010000531         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1154599,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000531 DASWGM010000000
VERSION     DASWGM010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154599"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VGA15_15445"
     CDS             complement(<1..389)
                     /locus_tag="VGA15_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9589127.1"
                     /translation="MADEFILETHGLTKEFAGFFAVRGVDLRVRRGSIHALIGPNGAG
                     KTTCFNLLTKFLKPSAGQILYKGRDITALAPADVARLGLVRSFQISAVFPHLTALENV
                     RVALQRQHGHSFDFWRSKTVLDRFNGRA"
     gene            742..1344
                     /locus_tag="VGA15_15450"
     CDS             742..1344
                     /locus_tag="VGA15_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589128.1"
                     /translation="MDFESANPTIIKSIKQRDLLNTWLRLYARAQAMPAIEEYQPERL
                     ADELPDLVYYTLDTARQPPRLLIQSDGTRMSSAYGNTGKGRYLDDFLGPRLAPVVMPV
                     YYECIKRALPVYTISDIDDIYGRIVAYERLLLPFSRGGDVTHVIASLKTISEDGGFEI
                     RNLMRGNDTLPTAKLRTVIDRDLFHRAPGRIPAGDVIEFS"
     gene            complement(1478..1855)
                     /locus_tag="VGA15_15455"
     CDS             complement(1478..1855)
                     /locus_tag="VGA15_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEX9589129.1"
                     /translation="MATITIYGIKNCDTMKKARAWLDGHGVAYGFHDYKTEGVAKDKL
                     KRWSDELGWETLLNRAGTTFRKLPDADKQGLDERKALALMLAQPSMIKRPVLDLGGKL
                     LVGFKPEIYAKEVAPSGRSARKA"
     gene            complement(1889..2728)
                     /locus_tag="VGA15_15460"
     CDS             complement(1889..2728)
                     /locus_tag="VGA15_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589130.1"
                     /translation="MDKIGGGKTGGQFRPNHHHTLKVNNTCSTQHGLHRTDVTVEASK
                     GVANMAQALQVSMELHGGEMHASSRVAILGRYRRYSDFRKDIQSAALGNVSESSFLAY
                     AKRIGLSDGKVVFADESELTLVYDLALYAAQPGRTRAIDRCARKRLTAAQPDEALVLR
                     ALQASRFSIFRVIGRCAPAGVLLEDLMRGGTIPLLDEGMERSAKPDDVFAMRVAPIED
                     FVITCGAIVPMDFETFEEIIEFLTDGAPDAELAALADRWRFAASLYELAILLELMSSI
                     IFR"
     gene            complement(2864..>3000)
                     /locus_tag="VGA15_15465"
     CDS             complement(2864..>3000)
                     /locus_tag="VGA15_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9589131.1"
                     /translation="QILARGEIPFLHVFSDNDSAIALYRRQGMEIRRRLCVTVLQQRI
                     "
BASE COUNT          610 a          944 c          864 g          582 t
ORIGIN      
        1 gcgcggccgt tgaagcggtc gagcaccgtc ttggaacgcc agaaatcgaa ggagtgcccg
       61 tgctggcgct gcagcgcgac cctgacattc tccagcgccg tcaaatgcgg gaacaccgcc
      121 gatatctgga acgagcgcac cagcccgaga cgcgccacgt cagccggcgc cagcgccgtg
      181 atatcccgtc ccttgtacag aatttggccg gccgaaggct tcaagaactt ggtcaggaga
      241 ttgaagcacg tcgtcttgcc ggccccgttg ggcccgatca acgcatgaat gctgccacgg
      301 cggacccgca gatcgacacc gcgaacggcg aagaaacccg caaattcctt ggtcagtccg
      361 tgggtttcaa gaatgaactc atcggccaat cagatttccc ccatcggccc tcgcgcccga
      421 catgccgtcg cgcgtggccc gttctcagct tgccggcagg ttcttgggac gcctcgaaaa
      481 tcccgtcccg gcccggccgc ctccggccgg aatatgccgt caacccgtcg gattaggcaa
      541 ggtggaaagc tgggcatgcg ccggcgcgcc cacggggtgc ggattcgcca ttagtcgaat
      601 tgcgcaaggg ggacgtttgg ccggttgcca acgcccggaa agcagccatt aacggtattt
      661 cgatgcaatt ttacgctttc atcaaaaatt cgcggcggag gcgcatggtt accgtgtttg
      721 catcaagaac ggattgccgc cgtggatttc gaaagcgcca accccacgat catcaaatcg
      781 atcaagcagc gtgatctgtt gaacacgtgg ttgcggcttt acgcccgcgc gcaggcgatg
      841 ccagctatcg aggaatacca gcccgagcgg ctcgccgacg aacttcccga ccttgtgtat
      901 tacaccttgg ataccgcccg gcagccgccg cgcttgttga tccagagcga cggcacgcgg
      961 atgtcgagcg cctatggcaa caccggcaaa ggccgctatc tcgacgactt tctcgggccc
     1021 cgattagcgc ccgtcgtgat gccggtctat tacgaatgca tcaagcgcgc cctccccgtc
     1081 tacaccatct ccgacatcga tgacatctac gggcgtatcg tcgcctatga gcggctgctg
     1141 ctgccgttct cgcgaggtgg cgatgttacc cacgtgatcg catcgctgaa gaccatcagc
     1201 gaagacggcg gctttgaaat caggaatctg atgcgaggaa atgacacgtt gccgaccgcg
     1261 aagctgcgca ccgttatcga tcgcgatctg tttcatcgcg cgcccggccg cattccggcc
     1321 ggcgatgtaa tcgaattcag ttaagcttca agctcaccaa cctgatggct atcaagccca
     1381 aagcggtgcc cgtgcatatc gtcacggccg tcatcgacct cgatttcgtg cccgacgctg
     1441 cgggccaccg cgcatcggac gatgttatcg aattgggcta agctttccgc gcggaacgac
     1501 ctgatggcgc cacttccttg gcgtagattt ccggcttgaa cccgaccagc aatttgccgc
     1561 cgagatcgag caccggccgc ttgatcatcg agggctgcgc cagcatcaag gcgagcgcct
     1621 tgcgctcgtc gaggccttgc ttgtcggcat cgggcaattt gcgaaacgtg gtgccggcgc
     1681 ggttgagcag ggtttcccag ccgagctcgt cgctccagcg cttgagcttg tccttggcga
     1741 cgccctcggt cttgtaatca tggaagccat aggccacgcc gtggccgtcg agccaggcgc
     1801 gggccttctt catggtgtcg cagttcttga tgccgtagat ggtgattgtc gccatatcct
     1861 gctctcaacc gattgctcaa gcgtcgcact atcgaaagat gatggaactc atcagctcaa
     1921 gcaggatcgc aagttcgtag agcgaggcgg caaagcgcca acgatcggcc agagcggcga
     1981 gttcggcgtc tggcgcgccg tcggtcagga actcgataat ctcttcaaat gtctcgaaat
     2041 ccatcggcac gatggccccg caagtaatca cgaaatcttc gatgggagcg accctcatcg
     2101 cgaaaacgtc gtcgggcttg gccgatcgct ccatcccctc gtccagaaga ggtatcgtgc
     2161 ctcctcgcat caaatcctca agcagaacac cggccggcgc acacctgccg atcactcgaa
     2221 agatggaaaa ccgggacgcc tgcaatgccc gcagaacaag agcctcgtcc ggctgcgcag
     2281 ccgtcaaccg ttttcgggcg cagcgatcga tggcacgcgt ccgccccggc tgggcggcgt
     2341 aaagcgcgag gtcgtagacc agcgtcagtt cgctctcgtc agcaaaaacc accttgccgt
     2401 ccgacaggcc gatccgtttg gcgtaggcaa gaaagctcga ctccgacacg tttccgagcg
     2461 ccgccgactg gatatccttg cgaaaatcgc tatagcgacg atagcgacca agaatagcga
     2521 cacggctgga ggcatgcatt tcgccgccgt gaagctccat tgatacctgc aatgcctgcg
     2581 ccatgttcgc tactcccttc gaagcttcga cggttacatc cgttcgatga agaccgtgct
     2641 gggttgagca cgtgttgttg actttaagcg tgtgatgatg attcggtcga aattgtccgc
     2701 ccgtttttcc cccgccaatt ttgtccacaa ctttcacgcg agcagtgcgg gacgcgtctc
     2761 gaattgacgc cgcaccaggc caacgcggcc gcgcaccatg tcagcttcgg gcagcgccgg
     2821 gggtcggaac accccgcgac agccggaatc cgggactggc acattagatt cgctgctgga
     2881 gcaccgtcac gcatagacgg cggcggattt ccatcccttg ccgccgatag agcgcgattg
     2941 ccgaatcatt atcagaaaat acgtgaagga acggaatctc gcctcgcgcc aggatctggc
//
LOCUS       DASWGM010000532         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1284417,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000532 DASWGM010000000
VERSION     DASWGM010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284417"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VGA15_15470"
     CDS             <1..350
                     /locus_tag="VGA15_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9589132.1"
                     /translation="VNEVFYRSFDNRRQVGGYFGLVGMPYDSGDSQRDQGISKAGNHR
                     ARELAVELAWLWIQHQPGSDLTLWFRKRVGDIKGRVRRIAIVAVARKLMVALWRYLET
                     GVVPTGAVLRPSL"
     gene            1084..>1737
                     /locus_tag="VGA15_15475"
     CDS             1084..>1737
                     /locus_tag="VGA15_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9589133.1"
                     /translation="MLTCLGAVAGHADKRVALVIGNSAYRNAPPLPNPRNDAEDVAAA
                     LKRTGFETIVGLDLDKAKMEDAAINFARAARTANVAVFYYAGHAIQYAGVNYLAPIDA
                     KLTDEADLRRMIRVDEIVADLQQAKNLRIPVLDSCRDNPLADQLKRAIGGSRTASIER
                     GLARIDSPQGMIIAYATQAGSTAEDGKGRNSPYTAAFLKHINEQDEIGTIFRRIAADV
                     "
BASE COUNT          338 a          566 c          505 g          328 t
ORIGIN      
        1 tggtcaacga agtgttctat cgcagcttcg acaatcgccg tcaggtcggc ggctatttcg
       61 ggctggtcgg aatgccctac gacagcgggg acagccagcg cgaccagggc atcagcaaag
      121 ccggcaatca ccgcgcccgc gaactcgccg tcgaactcgc ctggctgtgg atccaacatc
      181 agcccggcag cgatttgacc ctttggttcc gcaagcgggt gggcgacatc aaagggcgcg
      241 tacgacgcat cgccatcgtg gcagtggcgc gcaagttgat ggtcgccctg tggcgatatc
      301 tcgagaccgg cgtggtgccc accggcgccg tgctgcgtcc gagcctgtga acggccgcga
      361 cccatgatgg ccgacatgac ccaggacaga cgcgtgaccg tcctcatcca ggggcctaac
      421 caccccgctt tctcagatgg gtcccgtctc ctgtcggcct tgccttcgcc cgtgcatgaa
      481 gaattagggt ccgggccatc acgcccgacc ggatacaagg tgatgcggat ttttccgcag
      541 tccccactca cggccgagtc cagtgtcatg tccgcccaat cggcgcgccg cccatcgccc
      601 cgaccttcaa agcttcgcgc cgtgctcgcc gccgtcaagg cgtggcctgg agagccagag
      661 cctgccgcac ccgtgcagcg acggccagcc ttgacggcgg ctgcgcgcgg cgctccaccc
      721 caatcaaggt cgggacgaag aaacgcttcg gtcaaacaaa gaaacattcg cctgctgcac
      781 cgggagttcg cggcaatgcc ctaacgctac ctcttgaccc ccgtccccat acaaggatga
      841 ggtccgtggg gtccaattct atccacccga tctgattggt ttcacggaat cgattcagca
      901 ggagcatttt tccattaggt tgaccttgcc agtgtcattg ccctcccccg acttggtcgg
      961 gggattgacc gggcatccag tatccccggt cggtggttac ttgtggcata ctgctggcgg
     1021 acgatgaaac tcggcaaacg aggttcacgc atgcgtcttg tcctcgcttt cgcctgcgct
     1081 gtgatgctga cctgtctcgg agccgtggcg ggccatgccg ataagcgcgt tgcgctcgtc
     1141 atcggcaata gtgcctatcg caatgcccct cccctgccca atccgcgcaa tgatgccgaa
     1201 gacgttgcgg cggcgctgaa acggaccggc ttcgagacca ttgtcggtct cgatcttgat
     1261 aaagccaaaa tggaagatgc agcgatcaat tttgcccgcg ctgcccgcac cgcaaatgtt
     1321 gcggtcttct attatgccgg ccacgccatc cagtatgccg gggtcaatta tcttgcgccg
     1381 atcgacgcca aattgaccga cgaggccgac ttgcgccgaa tgatcagggt cgacgaaatt
     1441 gtcgccgatc tgcagcaggc caagaatctc cgcatccctg tgctcgattc ctgccgcgac
     1501 aacccgttgg cggaccagtt gaagcgcgcg atcggtgggt cacgaacggc atccattgaa
     1561 cgcggtctcg ccaggatcga cagtccgcaa ggcatgatca tcgcctacgc aacgcaggcc
     1621 gggagcacgg cggaggacgg aaagggtcgc aacagcccct acactgcagc ttttctgaaa
     1681 catatcaacg aacaggacga gatcggcacg atattccgcc gcatcgccgc cgacgtg
//
LOCUS       DASWGM010000533         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2138070,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000533 DASWGM010000000
VERSION     DASWGM010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138070"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VGA15_15480"
     CDS             <1..739
                     /locus_tag="VGA15_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9589134.1"
                     /translation="PSPPAVAYVFADGRGTEEIAGQLTGFSGILQVDGYAAYKALARG
                     HGGAIRLAFCLAHARRKFVEVYKTTQSPFAHEVIERLQAVYAIETEIRGCRAEQRLAV
                     RRARAAPLMEALKARLTTMLGQLFSQSPLAGAINYALNHWDGLTLFLDDGRVEVDTNT
                     VERSMRPIAMGRRNSLFSGSEGGAESWAILASLVNTAKLHELDPQAYLTDVLERIVSG
                     RTKSHQLHELLAWNWKAARERTARAAA"
     gene            736..1356
                     /locus_tag="VGA15_15485"
     CDS             736..1356
                     /locus_tag="VGA15_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEX9589135.1"
                     /translation="MSRRRHPAPSATTVDAMSLAELERWLRDRAGRRPVATSIATLDG
                     YVAAIVAGPVSISPLDWICPLLAIDADAFNHGGTAEFAAISAVVQHHNDISNTLSMAP
                     QLFKPIFRSKPGGGVDVRPWCHGFYSAMKLRLLAWSPLLSLDNPDYHLLLPVLLFCAD
                     DNGRPVVPGTDAAVVKRLRHQAPDDIPAFVAGIRQYWMPTRYKRTT"
BASE COUNT          330 a          558 c          519 g          330 t
ORIGIN      
        1 cccgtcgccg ccggcggtcg cctacgtgtt cgccgacggt cgcggcaccg aggagatcgc
       61 cgggcaattg accggcttct ccggcattct gcaggtggac ggctatgccg cctacaaggc
      121 gctggcgcgc ggtcatggcg gtgcgatccg actggctttt tgtctggcgc atgcccggcg
      181 caaattcgtc gaggtgtaca agacgacgca atcgccgttc gctcacgagg tgatcgaacg
      241 cctgcaggcg gtgtacgcca tcgagaccga gatccgcggc tgccgcgccg agcagcggct
      301 cgcagtccgt cgcgccaggg ccgcgccgct gatggaagcg ctcaaggcgc ggttgaccac
      361 aatgctcggc cagctgttct cacagtcgcc gctggccgga gccatcaact atgcgctcaa
      421 tcattgggac gggctgacgc tgttcctcga cgacgggcgt gtcgaggtcg acaccaatac
      481 ggtcgagcgt tcaatgcgcc cgattgcgat gggccgccgc aactcactgt tcagcggcag
      541 cgaaggcggc gccgagagct gggcgatcct ggcatcgctg gtcaacacgg caaagctcca
      601 cgaactcgat ccgcaggcct atttgaccga cgtgctggag cggatcgttt ccgggcgaac
      661 caaaagccat caactgcacg aactcctggc ctggaactgg aaggctgccc gcgagcgtac
      721 cgcacgggcc gccgcatgag ccggcgtcgc catccggctc cgtcggcgac gacggtcgac
      781 gccatgtcgc tcgcggagct cgaacgatgg ctgcgggacc gcgccgggcg gcggcctgtc
      841 gccaccagca ttgcgacgct ggacggctac gtcgccgcaa tcgtggccgg accggtatcg
      901 atcagcccgc tcgactggat ctgcccgctg cttgctatcg acgccgacgc attcaaccac
      961 ggtggcaccg cggagttcgc ggcgatctcc gccgtcgtgc agcaccacaa cgacatcagc
     1021 aacacgctct cgatggcacc gcagctgttc aagccgatct tccggtccaa acccggcggc
     1081 ggggtggacg tccgcccctg gtgtcacggc ttttactccg ccatgaaatt gcgcctgctg
     1141 gcatggtcgc cgctgctctc tctcgacaat cccgactacc acctgctctt gcccgtgctg
     1201 ctgttttgcg ccgacgacaa cggccgaccg gtcgtcccag ggaccgatgc ggctgtcgtg
     1261 aaacgacttc ggcatcaagc tcccgacgac attccggcat tcgtcgcggg aatccgtcaa
     1321 tactggatgc ccacccgata caagcggacg acgtaatcca tctacgtggc catctcgcac
     1381 cgcttacgga tgagtgggag atatgtcgta gctggattct gttgcggtga atcggcgtac
     1441 aatagctgtc acataaactg caaaaattgg actgatcagc aaggatagct caactctttc
     1501 ggcgctcgtc agcttgctcg tgctaccaga attagaaaaa atgaaaccag gtgtgatcca
     1561 atgatagaga aacccactat cattcttaat gtatctagct gcatcgtcct tctttcctaa
     1621 agattgcagg ctgccgtaaa agacggctcg gggggttctt tcaaggtgag tcctcgccgg
     1681 atgaggaacc catgaaaccc atcttgtatc tttccgagag catcatcgcc gagcttg
//
LOCUS       DASWGM010000534         8375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2280590,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000534 DASWGM010000000
VERSION     DASWGM010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280590"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1647)
                     /gene="hflX"
                     /locus_tag="VGA15_15490"
     CDS             complement(268..1647)
                     /gene="hflX"
                     /locus_tag="VGA15_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX9589136.1"
                     /translation="MEPRNPEEGVDRPRSAAGNATGRVIVIGPYLRMRRGDPEAQMDH
                     HVIRGSETRLEEAVGLARAIDLTIAEALIAPVSQIRPATYLGKGKVEEIVGLITGQEI
                     ELVVMDCALSPIQQRNLEKAWNTKVLDRTGLILEIFGRRAKTREGALQVELAHLNYQR
                     SRLVRSWTHLERQRGGFGFMGGPGETQIEADRRLIGDRIARLENEIKKVQATRRLHRA
                     GRQRVPYRVVALVGYTNAGKSTLFNRLTRADVQAADMLFATLDPTLRALSLPHGGKAM
                     LSDTVGFISNLPTQLVAAFRATLEEVLEADVILHVRDISHEDADAQQRDVDTVLRQLG
                     IDPDAGQRILEVWNKIDRFDPQMRENLQNIAARRPPERPCFLVSAETGEGIDALLTAI
                     EDRLAATRMTLDLTIDAADGAGISWLHRNSEVLNKELHDGRFDMTVRVDETKRDIVVN
                     RFDAVPHAM"
     gene            complement(1659..1907)
                     /gene="hfq"
                     /locus_tag="VGA15_15495"
     CDS             complement(1659..1907)
                     /gene="hfq"
                     /locus_tag="VGA15_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX9589137.1"
                     /translation="MAADRAQNLQDTFLNHVRKTKTPLTIFLVNGVKLQGIVTWFDNF
                     CLLLRRDGHSQLVYKHAISTIMPGAPIQLFEGGEDAPA"
     gene            complement(2221..3591)
                     /locus_tag="VGA15_15500"
     CDS             complement(2221..3591)
                     /locus_tag="VGA15_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9589138.1"
                     /translation="MANDILIVDDEADIRDLVAGILEDEGFTTRTARDSDSALAEIAS
                     RRPHLVFLDIWLQGSKLDGLQLLEQIKKDHAEVPIVMISGHGNIETAVAAIKRGAYDF
                     IEKPFKSDRLILVATRALETSRLKREVKELKQLAPAASVLTGRSACMNQLRQTIERAA
                     KANSRILIVGPSGAGKELAARTLHNASNRAEGPFVVINAAAITPERMEVELFGIEQSN
                     GEQPRKAGALEEAHGGTLFIDEIGDMPRETQNKILRVLVDQTFQRSGGTNKVNVDVRI
                     ISSTARNLEEEIAEGRFREDLYHRLSVVPIRVPPLSERREDIPELIDYFMDQISVATG
                     LPKRQIGQDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPEVIITADMLPQDVGSMV
                     PAMPTSNNGEHIMGLPLREAREVFERDYLIAQISRFSGNISRTAEFVGMERSALHRKL
                     KALGVG"
     gene            complement(3609..5933)
                     /locus_tag="VGA15_15505"
     CDS             complement(3609..5933)
                     /locus_tag="VGA15_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX9589139.1"
                     /translation="MTSADSPAPSFDPSFAESKGWSLRKWLAPFAVGLALLSAFLTFV
                     VLTGLTPIEPTREVVVSFMLINGATILVLVGIIVREVWQVVQARRRGRAAARLHVQIV
                     SLFSVIAVLPAVLVSIVANVTIDRGLDRLFSGPTRAAIENSLTIARAYTDEHAQLIRG
                     DILGMANDIARARPLYDQDRGTFVEMLTASAASRNLPGAMLIDKDSKPLETARTGIQQ
                     DFVTPDPELLSKVNETEPQIAVFPENYVAAVIRLRAFDDTFLYVARLLDPRVVAQLKQ
                     TQASVAEYAEIESRRLGLQVAFGLMFAVIALTILMASVLIGLNFANWLVAPIRRLMSA
                     ANIVSTGDLHVQVPVHKSEGDLAQLGETFNKMTQELRSQRDELVSASDVIDSRRRFIE
                     AVLSSASAGIIGVDGSGSVGILNRSAEKLIGHAESETLDHPLSDVLPELDDMMKTARE
                     GTQRLVQGQITILRDGHERNLSVRVSAEQTSQSRDSYIITLDDITELVSAQRTSAWAD
                     VARRIAHEIKNPLTPIQLSAERIRRKFGKVITEDKAVFEQCTDTIVRQVDDIRRMVDE
                     FSRFARMPKPVIEGEDVGDTVRQAVFLMRVGHPDIDIEAEIKDEPMRAQFDRRLISQA
                     LTNIIKNATEAIEAVPADELGKGRIDVIAARENDDIVIDVMDNGIGLPKVSRARLLEP
                     YVTTREKGTGLGLAIVGRVLEDHGGRIELNDAADIRPGARGAWMRLRFAVSGHAAKVE
                     VKEPAPETKQAVHETAGPADATNNETKIKTAAGG"
     gene            complement(6108..7550)
                     /gene="ntrC"
                     /locus_tag="VGA15_15510"
     CDS             complement(6108..7550)
                     /gene="ntrC"
                     /locus_tag="VGA15_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX9589140.1"
                     /translation="MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVS
                     QGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIVMSAQNTFMTAIRASERGAYEYL
                     PKPFDLKELIAIVGRALAEPKERVANHADESEFDSIPLVGRSPAMQEIYRVLARLMQT
                     DLTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFT
                     GANTRASGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRI
                     VAASNKDLRILIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPDLIRHFFALAEKDGL
                     PPKKLDALALERMKQHRWPGNVRELENLARRLAALYPQDVITGSVIDGELAPPAVTSG
                     GSVHHGVDNLGGAVEAYLSSHFSGFPNGVPPPGLYHRILKEIEVPLLTAALAATRGNQ
                     IRAADLLGLNRNTLRKKIRDLDIQVYRSGN"
     gene            complement(7560..>8375)
                     /locus_tag="VGA15_15515"
     CDS             complement(7560..>8375)
                     /locus_tag="VGA15_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9589141.1"
                     /translation="RTIADKMDRQLTHRSAARSVIALAAMLAHEIKNPLSGIRGAAQL
                     LEQAASSEDRMLTRLICDEADRIVTLVDRMEVFGDDRPVARGPVNIRSVLHHVKRLAQ
                     SGFARNIRFVEEYDPSLPPVLANQDQLIQVFLNLVKNAAEAVVDLGSDAEIQLTTAFR
                     PGVRLSVPGKKSRVSLPLEFCVKDNGPGVPEDLLPNLFDPFVTTKPTGSGLGLALVAK
                     IVGDHGGIIECESQPRKTTFRVLLPMFNSAKHFDHGNRDHASGTPLHASQDTR"
BASE COUNT         1514 a         2660 c         2540 g         1661 t
ORIGIN      
        1 atcgggccgc ggctttgctc cacgcttcct tcagaccccg cctcgcgacg acgcccttgc
       61 gctttgctaa tccttcgcca ccatcaggct ggataaagga ctttcacctc caagctgtcg
      121 atcatgctcg gcacacagcc accgggtcgg cgcaaagcgc cgcccgatga caggctccgc
      181 gaagcaatcc atgggagcaa caaggtaagc tggatcttcg tcgcttacgc tcctcgcaat
      241 gacgacggtt ctgacggcga aagtcgttca catcgcgtgc ggcaccgcat cgaaccggtt
      301 gaccacgata tcgcgcttgg tttcatcgac gcgcacggtc atgtcgaacc gtccgtcgtg
      361 aagctccttg ttgagcactt cggaattgcg atgcagccag ctgataccgg cgccgtcggc
      421 ggcgtcgatc gtaagatcca acgtcatgcg cgtggcggca agccggtctt cgatcgcggt
      481 caatagcgcg tcgatgccct cgccggtttc ggccgagacc aggaaacagg gacgctccgg
      541 cgggcggcgg gcagcgatat tttgcaggtt ttcccgcatc tgtggatcga agcgatcgat
      601 cttgttccag acctcgagaa tgcgctgacc cgcatccgga tcaatgccga gctggcgcag
      661 caccgtatcg acgtcgcgtt gctgcgcgtc cgcgtcctca tgcgagatgt cgcggacgtg
      721 gagaatgacg tcggcttcca gcacttcctc cagcgtcgca cgaaacgccg caaccagttg
      781 cgtcggcagg ttggaaatga agcccacggt atccgacagc atcgccttgc cgccatgcgg
      841 cagactgagc gcgcgcaagg ttgggtccag cgtggcgaac agcatgtcgg cggcctgcac
      901 gtcggcgcgg gtcaggcgat tgaacagcgt cgacttgccg gcattggtgt agccgaccag
      961 cgccacgacc cgatacggca cccgctgccg gccggcgcga tgcagccgcc gcgtcgcctg
     1021 aactttcttg atctcgtttt cgaggcgcgc gatgcggtcg ccgatcaggc ggcggtccgc
     1081 ttcaatttgc gtttcaccgg gaccgcccat gaacccgaaa ccgccgcgct ggcgctcgag
     1141 atgggtccat gaccgcacca ggcgacttcg ttggtagttc aaatgcgcga gttcgacctg
     1201 cagcgcgcct tccctggtct tggcgcggcg gccgaagatt tccagaatca gcccggtgcg
     1261 gtcgagtacc ttggtgttcc aggccttctc aagattgcgc tgctggatcg gcgacaacgc
     1321 gcaatccatc accaccagtt cgatctcctg cccggtgatc agtccgacaa tctcctcgac
     1381 cttgcccttg ccgagatacg tcgcaggccg gatctgactg accggcgcga tcagcgcctc
     1441 tgcgatcgtc agatcgatag cgcgggcgag acccacggct tcctcaagcc gcgtctccga
     1501 accgcgtatg acatgatgat ccatttgcgc ctcgggatcg ccgcggcgca tgcgcaagta
     1561 tgggccgatg acgatcaccc gcccggtcgc gttgcccgct gccgaccgcg gacggtcgac
     1621 gccctcttcg ggattacggg gttccaatca gatcactctc aagccggggc atcctcgccg
     1681 ccttcgaaca gctggatcgg tgcgcccggc atgatggtcg agatcgcgtg cttgtagaca
     1741 agctgcgaat gaccgtcgcg ccgcagcagc aggcagaaat tgtcgaacca agtgacaatc
     1801 ccctgcagct ttactccgtt gaccagaaag atcgtcagtg gcgtcttggt tttgcgaacg
     1861 tgattgagga aggtgtcttg taggttttgt gcgcggtctg ccgccattgt ttttatcccg
     1921 cagttttgtg cttctttttg tgcttctttt tattgaaccg gacgctggcc ctctcacgga
     1981 gcgtgacccg gttgccggct tccccctgag atccccctcc gggaggtacg gcaggacgat
     2041 tagaaggcag gcttggtttt taggcaagcc ggttcgcgca ttggccacag ccgcaattct
     2101 ccgtaagaat acggaaatag atgaaatttc tcaagaaaat gcatggaatg aatgcgtcga
     2161 cgcggtgttc aacgaccggc aaacggccgt atttcggatt cggaactctc gaaccggcat
     2221 tcagccgaca ccgagggcct tgagcttgcg atgcagggct gaacgctcca tgccgacaaa
     2281 ctccgccgtg cgggagatat ttccggagaa tcggctgatc tgggcgatca aatagtctcg
     2341 ttcgaacact tcccgcgcct cacgcagcgg caaccccatg atgtgctcgc cattattgct
     2401 ggtcggcatt gccgggacca ttgagcccac gtcctgcggc agcatatcgg cggtaatgat
     2461 gacttccgga ccgccgcccg ccaggatcat gacccgctcg acgttgttgc gaagctgccg
     2521 gacatttccg ggccagacgt gcgactgcaa taccgccatc gcgtcctggc cgatctggcg
     2581 cttcggtagc cccgtagcga ccgagatctg atccatgaaa tagtcgatca gttcggggat
     2641 atcctcgcgg cgctccgata acgggggcac acggatcggc accaccgaca accgatgata
     2701 gagatcttcg cggaaccgac cctccgcgat ctcttcttcc agattgcgcg ccgtcgagga
     2761 aatgatgcga acgtcgacat tgaccttgtt ggtgcccccc gaccgctgga acgtctgatc
     2821 gaccagcacc cgcagaatct tgttctgggt ttcgcgcggc atgtcgccga tttcatcgat
     2881 aaacaacgtg ccgccatgcg cctcctccag cgcaccggcc ttgcgtggct gttcgccatt
     2941 ggactgctcg ataccgaata gctcgacttc cattcgctcc ggcgtaatcg ccgcggcgtt
     3001 gatgacgacg aaaggtcctt cggcgcgatt cgatgcgtta tgcagcgtgc gcgccgccaa
     3061 ttcctttccc gcaccggacg gcccgacaat caggatgcgg ctgttggcct tggcggcccg
     3121 ctcaatggtc tggcgcagtt gattcatgca ggccgagcgg ccggtcaaaa cgctggccgc
     3181 cggcgcgagt tgcttgagtt ccttcacttc gcgtttcagg cgcgaggtct ccagcgcccg
     3241 cgtcgccacc agaatcaacc ggtcggactt gaagggcttt tcgataaagt cgtaggcgcc
     3301 gcgcttgatc gccgcgaccg cggtctcgat attgccgtgc ccggagatca tcacgatcgg
     3361 cacctcggcg tgatccttct tgatctgttc cagcagctgc aacccgtcga gcttgctgcc
     3421 ctgcagccag atgtcgagaa acaccagatg cgggcggcga ctggcaattt ccgcgagcgc
     3481 cgaatcgctg tcacgcgcgg tccgggtcgt aaaaccctcg tcttcgagaa tccccgcgac
     3541 cagatcgcga atatcggcct cgtcatcgac aatcagaatg tcatttgcca tcggtcacgc
     3601 ctgtctggtc agccgcctgc tgcggttttg atctttgttt cattgttggt cgcgtccgcc
     3661 ggccccgcgg tttcatgcac ggcttgtttt gtttcgggag ccggctcttt cacttcgact
     3721 ttcgcggcgt ggcccgacac cgcaaaccgc aaccgcatcc aggccccgcg cgcacccggt
     3781 cgaatgtccg cggcatcgtt gagctcgatc cggccgccat ggtcttccag aacgcggccc
     3841 acaatcgcca ggccgaggcc ggtgcccttt tcgcgcgtcg tgacgtaggg ttcgagcaga
     3901 cgcgcccggc ttaccttcgg caggccgatg ccgttgtcca tgacatcgat cacgatgtca
     3961 tcattctcgc gcgccgcaat aacgtcgata cgtcccttcc ccagttcgtc cgccgggacg
     4021 gcctcgatcg cttcggttgc gttcttgatg atgttggtca aggcttgcga tatcagccgc
     4081 cgatcgaact gcgcccgcat cggctcgtcc ttgatctcgg cttcgatatc gatgtcggga
     4141 tgtccgaccc gcatcaggaa taccgcctgc cgcacggtgt cgccgacatc ctcgccttca
     4201 atcacaggct tcggcatccg cgcgaagcgc gagaactcgt cgaccatccg cctgatatcg
     4261 tcaacctgtc gcacaatcgt gtccgtgcat tgctcgaaga cggccttgtc ctccgtgata
     4321 accttgccga atttgcgacg gatgcgttcg gcggagagct gaatcggcgt cagcggattt
     4381 ttgatttcgt gcgcgatgcg gcgcgcgaca tcggcccatg ccgaagtgcg ttgtgcggaa
     4441 accagttcgg taatgtcgtc aagcgtgatg atgtagctgt cgcgcgactg gctggtttgt
     4501 tcggcgctga cgcgaaccga cagattgcgt tcatggccgt cacgaagaat cgtgatctgg
     4561 ccctggacca gccgctgggt gccctcgcgc gcggttttca tcatgtcatc gagttcgggc
     4621 agcacgtccg acaagggatg atccagcgtt tccgattcgg cgtgcccgat gagtttctcg
     4681 gcggaacgat tgaggatgcc gacactgccg gagccatcga cgccgatgat gccggcgctc
     4741 gccgacgaca acacggcttc gataaaccgg cgacggctgt cgataacgtc gcttgcgctc
     4801 accaactcgt cgcgctggct acgcaattcc tgcgtcatct tgttgaaggt ctcgccaagc
     4861 tgcgcgaggt cgccttccga cttgtgaacg ggaacctgca catgcaaatc accggtcgag
     4921 acgatattgg cggcactcat cagccgccgg atcggcgcga ccagccagtt ggcgaaattc
     4981 agtccgatca ggaccgaggc catcaggatg gtcaaggcga tcaccgcgaa catcagcccg
     5041 aacgccacct gaaggccgag ccggcgcgat tcgatctcgg cgtattcggc aacgctggcc
     5101 tgggtttgct tgagctgcgc gaccacgcgc ggatcgagca gccgggccac atagaggaac
     5161 gtgtcgtcga acgcgcgcag ccggatcacg gccgcgacgt agttttcggg gaacaccgcg
     5221 atctggggct cggtttcgtt gactttgctc aggagttcag ggtccggcgt cacgaagtcc
     5281 tgctgaatgc cggttcgcgc cgtctccaga ggcttgctat ccttgtcgat cagcatcgct
     5341 cccggcaaat tgcgcgatgc ggcactggcc gtcagcatct cgacaaacgt tccgcgatct
     5401 tgatcgtaca atggccgggc gcgggcgata tcgttggcca ttcccagaat gtcgccgcga
     5461 ataagctgcg cgtgctcatc cgtgtaggcg cgggcaatgg tcagcgagtt ctcgatcgct
     5521 gcccgcgtcg ggcctgaaaa cagccggtcc aggccgcgat cgatggtcac attggcgacg
     5581 atcgacacca gcacggccgg caacaccgcg atgacggaaa acaggctgac gatctggacg
     5641 tgcagccgcg ctgccgcccg gccgcggcgg cgcgcctgca ccacctgcca gacttcgcgg
     5701 acgatgatgc cgaccagcac caggatggtc gcgccgttga tcagcatgaa ggaaacgaca
     5761 acttcgcgtg tcggttcgat cggggtcagg ccggtgagca ccacgaaggt gagaaatgca
     5821 gataacaggg ccaggccgac cgcaaacggc gccagccact tccgcaggga ccaccccttg
     5881 gattcggcga acgacggatc gaacgatgga gccgggctgt ctgcgctggt catttaggca
     5941 atgggtgagc tgaaaggcct tagatccgcc gctcgcggac tgatgcattc atatcacaat
     6001 gttgccaaat tgcgacgatt ccacggcgtg tcccgccgtt ggactgcact ggtctgcagt
     6061 tttcgccatc accataccgc tgcggccacc cgtcgggtcg cgcaacccta attaccgcta
     6121 cggtagacct ggatgtccag gtcacgaatc ttcttccgca gcgtgttgcg gttgaggccg
     6181 agcaggtcgg cggcgcggat ctggttgccc ctggtcgccg ccaacgccgc cgtcagcagc
     6241 ggcacctcga tttccttcag gatccggtga tagaggcccg gcggcggcac gccgttgggg
     6301 aacccggaaa aatgcgatga cagataggct tcgacggcgc cgccgagatt gtccacaccg
     6361 tggtggacgc tgccgcccga ggtcacggcg ggaggcgcca actcgccgtc aatgaccgag
     6421 ccggtgatga cgtcctgcgg atacaatgcc gcgagccggc gcgcgaggtt ttcgagctcg
     6481 cggacattgc cgggccagcg atgctgtttc atgcgttcca atgccagggc gtcgagcttc
     6541 ttcggtggca atccgtcctt ctcggccagc gcgaaaaaat gccggatcaa atcgggcaaa
     6601 tcctcgatcc gctcgcgcag cggcggcagg cgcaacggca cgacattcaa gcggaagaac
     6661 aaatcctcgc ggaacagacc ttgctgaatc aggatgcgca gatccttatt gctcgcggca
     6721 acgatgcgca cgtcggtctt gatcggcgtg cgaccgccaa cggtggtgta ctcgccctgt
     6781 tgcaggaccc gaagcagacg ggtctgcgcc tccatcggca tgtcgccgat ttcgtcgaga
     6841 aacagcgttc ccccctcggc ctgctcgaac cggcccgagg cgcgggtgtt ggcaccggtg
     6901 aaggcgccgc gttcatggcc aaacagttcg gactcaatga ggtcgcgcgg gatagccgcc
     6961 atattgaccg cgacgaaggg gccgttgcgc cgcttgccgt agtcgtgcag cgcacgtgcg
     7021 acgagctcct tgccggtgcc ggactcgccg gagatcatca cggtgagatc ggtctgcatc
     7081 agccgcgcca gcacccggta gatttcctgc atcgcgggcg aacggccgac cagcggtatg
     7141 gagtcgaatt cggattcgtc ggcgtggtta gccacccgct ccttcggctc cgccagtgcg
     7201 cggccgacga tggcgatcag ctccttcagg tcgaacggct ttggcagata ctcataggcg
     7261 ccgcgctcgg atgcgcggat tgccgtcatg aaggtgttct gcgcgctcat cacgatgacc
     7321 gggagattcg gccgcatctt cttgatccgc ggcagcaggt cgaatgcgtt ctcgtcgggc
     7381 atcacaacgt cagtgatgac cagatcgccc tcgccctggc tgacccagcg ccacaacgtc
     7441 gccgcgttcc cggtcaggcg cacctcgtat ccggcgcgcg agagggcctg gttcagaacc
     7501 gtacggatgg cggtatcatc gtcggcgaca agtatactac ctgcgggcat tgttattcct
     7561 catcttgtgt cctgtgaggc atgcaacggc gtccccgaag cgtgatcgcg attgccatgg
     7621 tcgaaatgct tggcgctgtt gaacatcggc agcagcacgc ggaaggtggt cttgcgcggc
     7681 tgggactcgc actcgatgat gccgccatga tcgccgacga tcttggccac cagcgcgaga
     7741 cccagcccgc tgccggtcgg tttggtcgtc acgaacggat cgaacaggtt cggcaacagg
     7801 tcctcgggca cgcccgggcc gttgtccttg acgcagaatt caagcggcaa cgacacccgc
     7861 gatttcttcc ccggcaccga cagccggacg cccggacgga acgcggtggt gagttggatc
     7921 tccgcgtcgc ttccaagatc gaccacggcc tcggcggcgt tcttcaccag attgaggaac
     7981 acctgaatca attggtcctg attcgcaagc accggcggca gcgacggatc gtattcttcg
     8041 acaaatcgga tgttgcgggc aaatcccgat tgcgccagcc gcttgacgtg atggagcacc
     8101 gaacgaatgt tgaccggacc gcgcgccacc ggccggtcat cgccgaacac ttccatgcga
     8161 tcgaccagcg tgacgatgcg gtccgcctca tcgcagatca gccgcgtcag catgcggtct
     8221 tccgacgacg ccgcctgctc gagcagctgt gccgcgccgc ggatgccgga cagcgggttc
     8281 ttgatttcgt gcgccagcat cgcggccagc gcgatcaccg aacgcgcggc acttcgatgg
     8341 gtcaattgcc ggtccatctt gtcggcgatg gtgcg
//
LOCUS       DASWGM010000535         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1422867,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000535 DASWGM010000000
VERSION     DASWGM010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422867"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VGA15_15520"
     CDS             complement(<1..972)
                     /locus_tag="VGA15_15520"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX9589142.1"
                     /translation="METLMLPFSPRFVVLTICAVVTALLLGIGIVDRKVFDLVLIPIL
                     IFGALTLLGIRDLMQKNHAVLRNYPISAHIRFLLEEIRPEMRQYFFESEKDGMPFSRD
                     TRALVYQRAKMVLDKRPFGTQEDVYREGYEWMHHSVAPKTKANEKFRITIGGPDCTRP
                     YSASVFNISAMSFGALSPNAVRALNAGAKKGGFAHDTGEGGVSPYHRENGGDLIWEIG
                     SGYFGCRNRDGSFDAEEFARVAGDDQIKMVELKVSQGAKPGHGGVLPAAKVSEEISRI
                     RGVAMDEDCISPATHRAFSTPLEMMAFIAEMRRLSGGKPAGFKLCIGH"
     gene            complement(1212..>1772)
                     /locus_tag="VGA15_15525"
     CDS             complement(1212..>1772)
                     /locus_tag="VGA15_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9589143.1"
                     /translation="QKLIDDHLPYRNLQDAKLPLHIVTTDIVSGDSVVLSEGSAAQAI
                     IASTAIPGAFAPVRYKDFYLADGAISSNTPIRVAVAKGANRLIILPTGYACATHAPPI
                     GAVANALHALTLLIARQLVSELEGLAPNIEYFVVPPLCPLVGSPYDFSRTSDHIERAI
                     QSTDAWLARKGMEQGGIPDEMRPHTH"
BASE COUNT          367 a          533 c          533 g          339 t
ORIGIN      
        1 atggccgatg caaagcttga atccggcggg cttgccgccg gacaaacgcc gcatctcggc
       61 gatgaaggcc atcatttcca gcggcgtcga aaacgcccga tgcgtggccg gcgagatgca
      121 gtcctcgtcc atggcgacgc cgcggatcct ggatatctcc tcggaaacct tggctgccgg
      181 caatacgccg ccatgcccgg gcttggcgcc ctggctgacc ttgagctcga ccatcttgat
      241 ctgatcgtcg ccggcgacac gggcgaattc ctccgcatcg aacgagccat cacggttgcg
      301 gcaaccgaaa tatccggagc caatctccca aatgagatcc ccaccatttt cgcggtgata
      361 gggactgacg ccgccctcgc cggtgtcatg cgcgaagccg ccctttttgg cacccgcgtt
      421 cagcgcccgc actgcgttcg gactaagcgc gccaaagctc atcgccgaga tattgaagac
      481 cgatgccgaa tacggcctgg tgcaatccgg accgccgatc gtgatacgaa atttctcgtt
      541 ggcttttgtc ttcggcgcca ccgaatggtg catccattcg tagccctcgc ggtagacatc
      601 ttcctgggtg ccgaacgggc gcttgtcgag caccatcttg gcgcgctgat acaccagggc
      661 gcgggtgtcg cgggaaaacg gcatgccgtc cttctcgctc tcgaagaagt actgccgcat
      721 ctccggccgg atctcttcca gcagaaaccg gatatgcgcc gagatcgggt agtttcgcag
      781 gaccgcatgg ttcttctgca tgagatcgcg aatgccgagc agcgtgaggg cgccgaaaat
      841 cagaatcgga atcaagacca gatcgaatac cttgcgatcg acgatcccga tgcccagaag
      901 caaggcggtg acgacggcgc aaatcgtaag aacgacgaag cgtggcgaga acggaagcat
      961 cagcgtttcc atgggacccc tccggcagac tcttgcctta ttattcttgt cgggcgcagc
     1021 ctattccaac aaccagaaca attttgtaca cgatatccgg cgcaaagctc gtggatatga
     1081 caaccgatag ctgcgaggct accgaatccc gcggcgaaca atcaatccgt cgtatgaccg
     1141 ggcggcatcg cgcgtctttt tgcggtgcaa cgtaattcgg ctgagcttcc ttcggctgag
     1201 catccaatgg ctcaatgagt atgcgggcgc atttcgtcgg gaatcccgcc ttgctccatg
     1261 ccctttcgcg ccagccacgc atcggtggac tggatcgcgc gttcgatatg gtctgatgtg
     1321 cgggagaagt cgtagggcga tcccaccagc ggacataatg gcggcaccac gaaatattca
     1381 atgttggggg cgaggccctc cagttcgctg accaattgcc gcgcgatcag cagcgtcagc
     1441 gcatgcagcg cgttggcgac ggcgccgatc ggaggcgcgt gggtggcgca ggcatacccg
     1501 gtcggcagaa tgataagccg gttcgcgccc ttcgcaaccg caacccggat cggcgtgttg
     1561 gaagagatcg cgccatcggc cagataaaaa tccttgtagc ggacgggagc gaacgcgccg
     1621 gggatcgcgg tagaggcgat gatcgcctgg gcggccgacc cctccgacag cacgacgctg
     1681 tcgccagaga cgatatcggt cgtgacgatg tgaagcggaa gttttgcatc ctgcagattg
     1741 cgatagggca gatgatcgtc aatcagcttc tg
//
LOCUS       DASWGM010000536         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2281215,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000536 DASWGM010000000
VERSION     DASWGM010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281215"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VGA15_15530"
     CDS             complement(<1..158)
                     /locus_tag="VGA15_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007798595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX9589144.1"
                     /translation="MGKVIGIDLGTTNSCVAVMDGKTAKVIENAEGMRTTPSIVAFSD
                     DGERLVGQP"
     gene            729..1649
                     /locus_tag="VGA15_15535"
     CDS             729..1649
                     /locus_tag="VGA15_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589145.1"
                     /translation="MFAVALLITAICPAAFAADPVFPPGVRVGMTPLVGLSRAKSFVG
                     FETEDQSVKVLVAELPAEAYGEVMNAFKANPAGAGGIKPESIETSAGLAYYTAENAKD
                     GANNVRRYSMILPGGTFSGYVAVQVPENASKIYTDEAIRQMFASAAIRKEVPVEEQLG
                     LMPFKLSELSDFKTVRTLAVGAAMILADGNEESGFEAAPFMVIGVIGAAPAQPEDRGR
                     FAQQAATTIPGVRDGRITMSEPIRIDGQPGYETRIDATSGKNNTAVTVVQWLRFGGSN
                     AVRIIGSAPRDEWAKAFPRFRAVRDGIQPR"
     gene            complement(1699..2319)
                     /gene="grpE"
                     /locus_tag="VGA15_15540"
     CDS             complement(1699..2319)
                     /gene="grpE"
                     /locus_tag="VGA15_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX9589146.1"
                     /translation="MTDPNRQNDDPVNPAQAGEPVVSKPYIMPDDPEAGSTEALAKEA
                     AELRDKVLRTLAEMENLRKRTTREVADARVYAISGFARDVLDIADNLQRALDAVPADT
                     KASADPGLKALIEGVELTERSLLNALEKNGVKKFDPSGEKFDPNFQQAMYEVPDPSVP
                     AGTVVQVVQAGYMIGERVLRPALVAVSKGGARVGSPANGNAPNDAA"
     gene            complement(2426..>2723)
                     /gene="hrcA"
                     /locus_tag="VGA15_15545"
     CDS             complement(2426..>2723)
                     /gene="hrcA"
                     /locus_tag="VGA15_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470809.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX9589147.1"
                     /translation="ALEDLERVRLLFDDLETKRGVIDLLGRAERAEGVRIFIGSENKL
                     FSLSGSSTIIAPYSDAAGHIVGVLGVIGPTRLNYARVIPTVDYAARIVSRMLGG"
BASE COUNT          521 a          854 c          847 g          501 t
ORIGIN      
        1 ggctggccga cgaggcgctc gccatcgtcg ctaaaggcga cgatcgatgg ggtcgtccgc
       61 atgccctcgg cgttttcgat gactttcgcc gtcttgccat ccattacggc gacgcacgaa
      121 ttcgtggtgc cgagatcgat cccaatgacc tttcccatgg tcctcatatc cttctttttg
      181 cggcaggttg gctgggccct gacggcacgc caatccaacc ccccaaacga tcaaatgctc
      241 gcgatatcgc gatggtgagc ctcatatagg agggggggtg gtccctgcaa ggaccgggac
      301 gcaaccttag cccacgaaaa ctccaacctt tgaaagatcg tgtcccataa gaccgcagcg
      361 gggcgcgagg cgcggccgcg ttaagagata gacgacgaag gccggggcga tcagcgccgg
      421 cccgggccgg tgactggtcc cccgacgcaa gccgcaatgc gggggtccag tacaggggcc
      481 gcaccaggcg cccgccggga gcgtcacgcc tcgcaccggc gagcggcgcc accaggcgcc
      541 aaaaagcggc gatttccgcc ggtaacgatg tgtcacggag tcgcgacctg gtctgttgct
      601 ggagcggcaa aacggctaaa agcgagcgga ctgaagacgc ggccatcgct aaaacggccg
      661 tgaccggccg accattgatc ggcctcaatg caaacccagt agattttttc atgaagccca
      721 ttcgactgat gttcgcggtt gcgctgctca tcacagccat atgtcccgcc gcttttgccg
      781 cggaccctgt tttccccccc ggggttcgcg tcggcatgac gccccttgtg ggcctctcgc
      841 gcgcaaaatc attcgtcgga ttcgaaaccg aggatcagag cgtcaaggta ttggtggcag
      901 agctgccggc cgaggcctat ggcgaagtca tgaacgcctt caaggccaat ccggcaggcg
      961 cggggggcat caagccggag agcatcgaga cgtcggccgg acttgcctac tacaccgccg
     1021 aaaacgccaa ggatggcgcg aacaatgtgc ggcgctattc gatgattctg ccaggtggga
     1081 ctttttccgg ctatgtcgcc gtccaggttc ccgagaatgc ctccaagatc tataccgacg
     1141 aggcgatccg gcagatgttt gcctcggcgg cgatccgaaa ggaggttccg gtcgaggagc
     1201 agcttgggct gatgccgttc aaactcagcg aactcagcga tttcaagact gtccgcacgc
     1261 tggcggttgg cgctgccatg attctggcag acggcaatga agaatcaggt tttgaggccg
     1321 cccccttcat ggtgatcggc gtgatcgggg cggcgccggc ccagcccgag gatcgcggcc
     1381 gttttgccca acaggcggca accacgattc ccggggtacg cgatgggcgc attacgatgt
     1441 ccgagcctat tcgcatcgac ggccagcccg gatatgaaac gcgcatcgac gccaccagcg
     1501 gcaagaacaa taccgccgtc accgtggtgc aatggctgcg attcggcggc tccaacgccg
     1561 tccgcatcat cggaagcgcc ccgcgcgacg aatgggcgaa agcatttccg cgcttccgcg
     1621 ccgttcgcga cggcattcag ccgcgctgat ccggcaatcg cattatcttg cccgatcgcc
     1681 tgatccgaat aatgggtgtc aggccgcgtc gttcggcgca ttgccgttgg ccggcgatcc
     1741 gaccctggcg ccacctttgg agaccgcgac gagagccggc cgcaacaccc gctcgccgat
     1801 catgtagccg gcctgcacga cctgtaccac cgttcccgcg ggtaccgagg ggtcgggcac
     1861 ctcatacatc gcctgctgaa aattcggatc gaacttctcg cccgagggat cgaatttctt
     1921 gacgccgttt ttctccagcg cgttgagaag cgagcgctcg gtgagctcga caccttcgat
     1981 cagcgctttg aggccgggat cggctgacgc cttggtgtcg gccggaaccg catcgagcgc
     2041 acgctgcaga ttgtcggcga tatcgagaac gtcgcgcgcg aaaccggaga tggcataaac
     2101 ccgggcatcg gccacttcgc gcgtggtgcg cttgcgcagg ttttccattt ccgccagcgt
     2161 gcgcagcacc ttgtcgcgca attccgcggc ttccttggcg agcgcttcgg tcgatccagc
     2221 ttcgggatcg tcgggcatga tgtagggctt ggacaccacg ggctcgccgg cctgcgccgg
     2281 gttcaccgga tcatcattct gccggttggg atcggtcata gactgccttc ttgaagtcga
     2341 cggggatcga tggggaggtt gctggggcgg gatatcgtgc tttaggcgta gaaaatcaag
     2401 cgcgaccagc ccggtacagg tgagatcatc cgcccagcat tcggctgacg atgcgcgccg
     2461 catagtccac ggtcggaatc acccgggcat agttcagccg cgtcggtccg atcacgccga
     2521 gaacgccgac aatatgaccc gcggcatcgc tatagggtgc aataatggtg gaggagccgg
     2581 acagcgaaaa cagcttgttt tccgacccga tgaaaatccg cacgccttcg gcgcgctcgg
     2641 cgcgtcccag cagatcgatc acgccgcgct tggtctcgag atcgtcgaac agcaacctga
     2701 cccgctcgag atcttcaagc gca
//
LOCUS       DASWGM010000537         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1155383,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000537 DASWGM010000000
VERSION     DASWGM010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155383"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /locus_tag="VGA15_15550"
     CDS             complement(<1..1988)
                     /locus_tag="VGA15_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX9589148.1"
                     /translation="MAIEAMIQTMREAEVVSESPFYIPMTGPAARPRRALKHDDTFVV
                     LDSHGDIGASAGGPDGLFNADTRYLARLELVLDGVQPLLLGSNLRDDNSALTVDLTNP
                     DVYSNDRIVLQKDMLHIVRTVFLWRGTAYQRIGVQNHGETTASFDLTLLFDNDFADLF
                     EVRGERRPRRGIGSSKLLGPADVALEYNGLDGSSRSTAVQFDPRPTRLATNAATYHLD
                     LAPQQVTALFVSVACNKPAALKPAPFFRGLLAHRREMRRSTAGATSIETSNNIFNEVL
                     CQSMADLNMLMTETPQGRYPYAGIPWYSTTFGRDGLITALQMLWIDPRVAQGVLKRLA
                     FYQARTVDPLADAEPGKILHEMRGGEMAALREVPFAQYYGSVDATPLFVLLAGLYVER
                     TGDDATLAELWPAIESALRWIDGAGDPDQDGFVEYQRASEQGLANQGWKDSYDAIFHA
                     DGRLAEGYIALAEVQGYVFAGKRLAARCALRLGLVDKARKLEAEAQRLAERFEDAFWC
                     EELGTYALALDGAKQPCRVQTSNAGQLLFTGIVRHDRARMVAADLMRPHFFTGWGIRT
                     VARGQARYNPMSYHDGSIWPHDNALIALGLARYGLKHSVEQVFKGLFDAASYMDLRRL
                     PELFCGFQRERRRGPTLYPVACAPQAWASATPFTLLEAA"
BASE COUNT          376 a          630 c          634 g          359 t
ORIGIN      
        1 gctgcttcca gcagggtgaa cggcgtcgcg cttgcccagg cttgcggcgc gcaagcgacc
       61 gggtacagcg tcgggccgcg gcgcctttca cgctgaaatc cgcagaacaa ttcgggcagc
      121 cgccgcaaat ccatatagct cgcagcgtcg aacaggccct tgaacacctg ctcgaccgaa
      181 tgcttcaacc cgtaccgcgc gaggccgagt gcgatcagcg cattgtcgtg gggccagatc
      241 gacccgtcgt gataggacat cggattgtag cgcgcttggc cgcgcgcgac ggtgcggatg
      301 ccccaacccg tgaaaaagtg cggccgcatc aggtcggcgg cgaccatgcg cgcgcggtca
      361 tggcgcacga taccggtaaa caaaagctgc ccggcattcg acgtctgcac cctgcatggc
      421 tgcttggcgc cgtcgagtgc cagcgcatag gtgccgagtt cctcgcacca gaacgcatct
      481 tcaaatcgct cggcgagacg ttgcgcctcg gcttccagct tgcgcgcttt atccaccaaa
      541 ccgaggcgga gagcgcaacg tgccgccaac cgcttgccgg caaacacata gccctgaact
      601 tcggcgagcg cgatgtagcc ctcggcaagc cgtccgtcgg catggaaaat ggcgtcgtag
      661 gaatccttcc agccctggtt ggccaatcct tgttcggagg cgcgctgata ttcgacaaat
      721 ccgtcctgat cgggatcgcc cgcaccgtcg atccagcgca acgcggattc gatcgccggc
      781 cataattcgg ccagcgtcgc atcgtcgccg gtgcgttcga catagagccc ggccagcagc
      841 acgaacagcg gcgtcgcatc gacgctgccg tagtattgcg cgaacggcac ctcgcgcagc
      901 gccgccattt cgccgccccg catttcatgc aggatcttgc cgggctcggc atcggcgaga
      961 ggatcgacag ttctggcttg atagaatgcg agccgcttca gcacgccctg cgcgacgcgc
     1021 ggatcaatcc acagcatctg caatgccgta atcaggccgt cgcggccgaa ggtcgtcgaa
     1081 taccagggaa tgcctgcata gggataccgg ccttgcggcg tctccgtcat cagcatgttg
     1141 aggtcggcca tcgactggca cagcacctcg ttgaaaatat tgttggaggt ctcgatgctg
     1201 gttgcgcctg ctgtcgatcg gcgcatctcg cggcggtgcg ccagcagtcc gcgaaagaac
     1261 ggcgccggct tgagcgcggc cggcttgttg caggcaaccg acacgaacag cgcggtaacc
     1321 tgctgcggcg ccagatcgag atgataggtg gcggcgttgg tcgccagccg cgtcgggcgc
     1381 ggatcgaact gaacggccgt actgcgtgat gagccgtcca gcccgttgta ttccagcgcg
     1441 acgtcggcgg ggccaagcag tttacttgag ccgatgccgc gccgtggacg gcgctcgccg
     1501 cgcacttcaa acaaatcggc gaagtcattg tcgaacagca gcgtcagatc gaagctcgcg
     1561 gtggtctcgc cgtggttctg cacgccgatc cgttgatagg cggtcccgcg ccacagaaag
     1621 accgtgcgta cgatgtgcag catgtccttc tgcagcacga ttcggtcgtt gctatagaca
     1681 tcgggattgg tgagatcgac cgtcaacgcc gagttatcat cgcgcaggtt cgatccgagc
     1741 agcagcggct ggacgccgtc cagcaccagt tcaagtcggg cgagatagcg ggtgtcggca
     1801 ttgaagaggc catcggggcc gccagcggat gcgccaatgt cgccatggct gtcgagcaca
     1861 acgaaggtgt cgtcatgctt gagcgcgcga cgcggtcgcg ccgccggtcc ggtcatcgga
     1921 atgtagaacg gcgattccga gacgacttcg gcctcacgca tcgtttgaat catagcttcg
     1981 attgccatcg ggtgacctt
//
LOCUS       DASWGM010000538         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2281540,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000538 DASWGM010000000
VERSION     DASWGM010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281540"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VGA15_15555"
     CDS             complement(<1..536)
                     /locus_tag="VGA15_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589149.1"
                     /translation="MPKSLLRIGPQLIVVLDDGASYHIAKGMFVLVVGFYWLADCQVR
                     TMKHIVENLLLFLICTGTIVLFNSSPGYALDQSQAIENCRSSSGKPAYVACKQGGGTH
                     EACFGKAKAIVQSCVRSALIAARPKAALFSAEKLSQPASQAAPSAADVAKDASASLVA
                     PPRTISDISAILDQQKPDP"
     gene            complement(890..2683)
                     /locus_tag="VGA15_15560"
     CDS             complement(890..2683)
                     /locus_tag="VGA15_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEX9589150.1"
                     /translation="MASLDSVSIAILLGAVLVMAGILSSLLALRFGAPLLLVFLFIGM
                     IAGDSGPGQLRFDDVRTTYLVGSVALALILFDGGLRTRFQSIRTVLAPSMALATIGVL
                     LTALITAPVAKYALDLNWTEALLVGAVVASTDAAAVFLLVHAQGLRLRPRVGATLEVE
                     SGTNDPFAVFLTLMLVELISIGESSALHVALEFGREAVLGAIVGITGGRLVVLSLNRV
                     ALPQGLHAPFVATAALVIFGAAQIGHASGFLAVYLAGIIIGNRPTRAHNSVVTFLDAA
                     TWLAQIVMFVLLGLLVSPQRLVVSIIPSVIVALVLMLVARPLAVFLCLAPFRFSWREK
                     VFIAWTGLRGAVAIFLASIPMLVGLSKAYLYFDVAFVVVIISLLLQGWTLAAAARRLH
                     VALPRADRGPRRVELDLPGQLEQQLVGYSVRPKSLYFRRGLIPSWSKPTLVIRDQHIL
                     SPVEAEPIAPGDYIYLLAPPEKAQALDRFFVDMLPSTAPDPHLLGDFVVSGEHTLGEL
                     AGIYGVVVDAEQAKLTLSDYFDVHLDRAPKDGATLALDSIVLVARSISGGRVNVVGLR
                     LPEEEEEAAPPTRRARLKRKLSEMWASVAGI"
     gene            2926..>4232
                     /locus_tag="VGA15_15565"
     CDS             2926..>4232
                     /locus_tag="VGA15_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX9589151.1"
                     /translation="MTMDLNDIPEFLLSAARSVGAEITSPWFYLQLGLILTGAGIGYA
                     AGAAIRSRIEMSSLAMGWPAPLRLFMRVLVGSASTAVFAVLMTLARVVMLTSTWPSRS
                     YLLSVAANLAFAWLIIRLVTSVIRNEFIVRLVSLSAWLVAALSILGQLDPTIEALDSV
                     SVVLGGLRLTPLLLIKLAVLLAVALWLTNIASNFVESRITRSGDLTPSIQVLLIKMIR
                     LALMIFAVAVVMSAVGINLSALAIFSGAAGVGIGFGLQKIVANFISGIILLADKSVKP
                     GDLVTIGDSSGRISAMKTRYISVAAGDGREFLIPNEDLVTQKVTNWTYTDKNTLVKVN
                     FGTNYDADPRLVCKLAIDVAAAAPRAIKGKPPNCILVEFAEVGMKFSLTFWIADPDGM
                     DNVKSDVMLSLWEVFKREGIRVPYPVREIRIRGGALPVETTVEV"
BASE COUNT          868 a         1337 c         1275 g          752 t
ORIGIN      
        1 ggatccggct tttgttgatc gagaatggcg ctgatgtcgg agatggttcg cggcggagcg
       61 acaagacttg ccgaggcatc cttcgcgacg tccgcggcgc tgggcgcggc ttgagaagcc
      121 ggctgcgaca gtttctctgc cgagaacaaa gcagcctttg ggcgagccgc gatcagtgcg
      181 ctcctgacgc aggattgaac aatcgcttta gccttgccga agcaggcttc gtgggttcct
      241 ccgccttgct tgcaggccac gtaggctggc ttgcccgacg agctacgaca gttctcgatc
      301 gcctgacttt gatcgagagc gtatcccgga gaactgttga aaagtacaat ggtccccgtg
      361 caaatcaaga acagcaaaag attttccacg atgtgtttca tcgttcttac ctggcaatca
      421 gctagccaat aaaatccaac aaccaataca aacatacctt tcgcaatatg atagcttgct
      481 ccatcatcaa gcaccacaat caattgaggt ccgatgcgca gtaggctttt gggcaactat
      541 cgatttcggt atgctccctt aggtctccag gattgcgccg gatcaagcca atactttgcg
      601 gagagattta agcaacgcat ttcgcttgcg atcgctgccg tcgtatatct caagcgtcag
      661 cgttgttgac gcaacaatag agccgccaca gtcaactgtc cccatgagat ttgctcgcag
      721 caatgcgcga agactttaaa gccaaatgac tgcgctgctc caagtggtgc agtgatgcga
      781 tcacggagcg aaagaatatc ggacagtgca ggcgataagt gcgcgcgacc attccgtgca
      841 acgaggcttt caagcgacgt ccggcagtgc acgcatccag tgcagtgccc taaatgcccg
      901 ccaccgatgc ccacatctcc gagagcttgc gcttcaaccg cgccctccgc gtcggcggcg
      961 cggcttcttc ctcctcttcg ggcaggcgaa ggccgacaac attgaccctg ccgccgctga
     1021 tgctgcgcgc caccagaacg atggagtcga gcgcgagcgt cgcgccgtct ttcggcgcac
     1081 gatcgagatg cacgtcgaaa taatctgata gcgtgagctt cgcctgttct gcatccacca
     1141 ccacgccgta gatgccggca agctcgccaa gcgtgtgctc acccgagacg acaaaatctc
     1201 cgagcagatg cgggtcaggc gcggtgctcg gcagcatgtc gacaaagaaa cgatccagcg
     1261 cttgggcctt ttcgggcggc gccagcagat aaatgtagtc gccgggcgcg atcggctcgg
     1321 cctcgacggg cgagagaata tgctggtcgc ggatgaccag cgtcggcttc gaccaggacg
     1381 gaatgaggcc acgccggaaa tacaggcttt tcggacggac ggaataaccg acgagctgct
     1441 gctcgagctg gccgggcaga tcaagttcga cacgccgcgg cccgcggtcc gcgcgcggca
     1501 gcgccacatg cagccgccgg gctgccgcgg ccagcgtcca gccctgcagc agcagcgaga
     1561 tgatgacgac gacaaaggcg acgtcgaaat agaggtacgc cttcgatagt ccgaccagca
     1621 tcgggatcga tgccagaaag atcgcgaccg caccgcgcag gccggtccag gcaatgaaaa
     1681 ctttctcccg ccagctgaag cgaaacggcg ccagacagag aaacaccgcg agcggccgcg
     1741 ccaccagcat cagcaccagc gccacgatca ccgaggggat gatgctgacg accaggcgct
     1801 gcggcgagac cagcaggccg agcagcacga acatcacgat ctgcgccagc caggtcgcgg
     1861 cgtcgaggaa cgtcaccacc gaattgtgcg cccgggtcgg gcgattgccg atgatgatac
     1921 cggcgagata gaccgcgaga aatcccgacg cgtgcccgat ctgcgccgca ccgaaaatca
     1981 ccagggcggc ggtggccaca aacggcgcgt gcaggccctg cggcagcgcc acccgattaa
     2041 gcgagagcac caccagccgg ccgccggtta tgccgacgat ggcgccgagc acggcttcgc
     2101 ggccgaattc cagcgcgaca tgaagggccg agctttcgcc gatcgaaatc agctcgacca
     2161 gcatcagcgt gagaaatacc gcgaacggat cgttggtgcc ggattcgacc tccagcgtcg
     2221 cgccgacccg cggacgcagc cgcaagccct gcgcatgcac cagcagaaac accgccgcgg
     2281 catcggtcga cgccaccacg gcgcccacca gcagcgcttc ggtccagttc agatcgagcg
     2341 catatttggc gaccggtgcg gtgatcagcg cggtcagcaa cacgccgatc gtcgccagcg
     2401 ccatcgaggg cgccagcacc gtgcggatgc tttgaaaccg cgttctcaaa ccgccgtcga
     2461 acaggatcag cgccagcgcc accgagccca ccagataggt ggtgcgcacg tcgtcgaacc
     2521 gcagttgccc ggggccggag tcgccggcga tcatgccgat gaacaagaac accagcagca
     2581 acggcgcgcc gaaccgcagc gccagcaggc tcgacagaat gccggccatc accagaaccg
     2641 cgccgagaag aatggctatg ctgaccgaat cgagagaggc catgagcttt cgcaatcatt
     2701 gcagatactt ggcaaatact agcgcaaacg ctgcgatcgc atccttatcg tcgccacagt
     2761 tcgacgccaa cccatttctc tcatcagcgg caatgtgccc gatttgctgg cttaacttcc
     2821 tgcattggcg tgttgtatcg atggcgccgg cgccgcggtt atggaaacct gcgggatatt
     2881 cgaatcaagt tgatctgatg gtgccgatcg gccctgcgat gagatttgac catggacctg
     2941 aatgacatcc ccgaatttct gctgtccgcc gcgcggtcgg tcggcgcgga aatcacgtcg
     3001 ccatggttct atctgcagct ggggctgatc cttaccggcg ccggcatcgg atacgccgcg
     3061 ggcgccgcca tccgctcgcg gatcgagatg agctcgctgg cgatggggtg gccggcgccg
     3121 ctgcggctgt tcatgcgcgt gctggtcggc agcgcctcga ccgcggtgtt cgcggtactg
     3181 atgacgctgg cgcgcgtggt catgctaacg tcgacctggc ccagccgcag ctatctgctc
     3241 agcgtcgccg ccaaccttgc cttcgcctgg ctgatcatcc ggctcgtcac ctcggtcatc
     3301 cgcaacgaat ttatcgtccg cctggtgtcg ctgtcggcgt ggctggtcgc ggccctgagc
     3361 attctcggcc agcttgatcc gaccatcgag gcgctcgact cggtctcggt cgtgctggga
     3421 ggcctgcggc tgacgccgct gctgctgatc aagctcgccg tgctgttggc ggtggcgctg
     3481 tggctgacca atatcgccag caacttcgtg gaaagccgga tcacgcgatc gggcgacctc
     3541 acgccatcga tccaggtgct gctgatcaaa atgatccggc tcgccctgat gatatttgcc
     3601 gtcgcggtcg tcatgagtgc ggtcggcatc aacctttcag cgctggcgat cttctcgggt
     3661 gcggccggcg tcggcatcgg cttcggcttg cagaagatcg tcgccaattt catcagcggc
     3721 atcattctgc tggccgataa atcggtaaaa cccggcgatc tcgtcaccat cggcgacagt
     3781 tcgggacgca tcagcgcgat gaagacccgc tacatttcag tcgccgccgg cgacggccgc
     3841 gaattcctga tcccgaatga ggatctggtg acgcaaaagg tcaccaactg gacctacacc
     3901 gacaagaaca cgctggtgaa agtcaatttc ggcaccaatt acgacgccga tccgcgactg
     3961 gtctgcaaac tggcgatcga cgtcgcggcc gccgcgccgc gcgccatcaa gggcaagccg
     4021 ccgaactgca ttctcgtcga attcgccgag gtcggcatga agttttccct caccttctgg
     4081 atcgccgatc cggacggcat ggacaacgtc aagagcgacg tgatgctgtc gctgtgggaa
     4141 gtcttcaagc gcgagggcat ccgggtgccc tatcccgtgc gcgaaatccg gatccgcggc
     4201 ggcgcgctgc cggtcgagac cacggtcgag gt
//
LOCUS       DASWGM010000539         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1155568,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000539 DASWGM010000000
VERSION     DASWGM010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155568"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VGA15_15570"
     CDS             complement(<1..902)
                     /locus_tag="VGA15_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX9589152.1"
                     /translation="MTRRIGPSAKMRTSAVIMAAALLLAGEARAQSSGGGFLNFFDNI
                     FTGSVPKAGQSAPSGQPPGTSAPAQTAPAAGGAAPPWSGEDGASGHPLMTAGAIREAA
                     ANFDKCVASMWPDAARRNISQESFQRFTAGLEPDLRIMDLMDSQPEFTKAIWDYLDIL
                     VNDNRLAKGREILAKYKPQFDAAEKTYGVDRYSIAAIWGIESNYSTQMGDRSVLQSTA
                     TLACIGRRQKYFKDEFLSALEILHHGDLRPEQMRGSWAGAFGPTQFMPTAFKRYAVDA
                     DGDGRRDVVDDAADLIASTANNLK"
     gene            1318..>1728
                     /locus_tag="VGA15_15575"
     CDS             1318..>1728
                     /locus_tag="VGA15_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX9589153.1"
                     /translation="MKIRKAVFPVAGLGTRVLPATKAMPKEMLTIVDKPLIQYVVDEA
                     KEAGIEHFIFVTGRNKGVIEDHFDRMYELDATLAARGKKAEMEILARDQPEAGAMSFT
                     RQQAPHGLGHAVWCARDIVGNEPFAVVLPDELVLN"
BASE COUNT          319 a          539 c          541 g          329 t
ORIGIN      
        1 ttcttcaaat tgttggcggt cgacgcgatc aggtcggcgg cgtcgtcgac gacgtcgcgg
       61 cggccgtcgc cgtcggcatc caccgcgtag cgcttgaagg cggtcggcat gaattgcgtc
      121 gggccaaacg cgccggccca ggagccgcgc atctgctccg ggcgcaggtc gccatggtgc
      181 aggatttcca gcgccgagag gaactcatcc ttgaaatatt tctggcgacg gccgatgcag
      241 gccaaagtcg cggtcgactg cagcacgctg cgatcgccca tctgggtgga ataattcgat
      301 tcgatccccc agatcgccgc gatgctgtaa cggtcgacgc cataggtttt ttccgccgcg
      361 tcgaattgcg gtttgtattt ggccaggatc tcgcggccct tggcgaggcg attgtcgttg
      421 accaggatgt cgaggtaatc ccagatcgcc ttggtgaatt cgggttgcga atccatcagg
      481 tccataatgc gcaaatccgg ctcaagcccc gcggtgaagc gctgaaaact ctcctgcgag
      541 atgttgcggc gtgcggcatc aggccacatc gaggcgacgc acttgtcgaa attcgcggcc
      601 gcctcgcgga tcgcgcccgc ggtcatcaag ggatggcctg aggcgccgtc ctcgccgctc
      661 cagggcggtg cggcgccgcc ggcggccggc gcggtttgcg ctggcgccga agtgccgggc
      721 ggctgtcctg acggagcgct ctggccggct ttcgggaccg atcccgtaaa aatgttgtcg
      781 aaaaaattca ggaacccgcc gccggaagac tgcgcgcgtg cctcgccggc aagcagcagc
      841 gccgccgcca tgatgacggc gctggttcgc attttcgccg atggtccgat ccgtcgcgtc
      901 atgtctctca cccgggctgg tctcgatatc ccgccctcag gaggccttgt cagggtttcc
      961 aataacttaa caaaagcgag attttgttgt ccggaatctt gccttgtggt gagggacgcg
     1021 aggaaatgcc aacggtgccg cgggcaagac ctgctctctc cccccgcaac cggggtctac
     1081 cccggttgcg catttcaaat cttgcgcaag tcgggcaggc ccgacttgcg caaaataaaa
     1141 aacaacccgg gcaagcccgg gttgttgggg ggagggaaca gaaccaagct gccgatttac
     1201 cgtacccttg aggaagtttc gctctcgaca catcagcctt tgttcgcgga tgcaaaccgg
     1261 ctaccaacgt gcgaacctta ctttccgcac gctctcttaa tggcatcccg ataccgcatg
     1321 aaaatccgca aagccgtttt tcctgtcgcc ggcctcggca cccgcgtcct gcctgccacc
     1381 aaggcgatgc cgaaggaaat gctcaccatc gtcgacaaac cgctgatcca gtatgtggtc
     1441 gacgaggcca aagaggccgg catcgagcat ttcatcttcg tcaccggccg caacaagggc
     1501 gtcatcgagg atcatttcga ccggatgtac gagctcgacg ccacgcttgc cgcgcgcggc
     1561 aagaaggccg agatggagat tttggcgcgc gatcagcccg aggcgggcgc catgagtttc
     1621 acgcggcagc aggcgccgca cggcctcggc cacgcggtgt ggtgcgcccg cgacatcgtc
     1681 ggcaacgagc cgttcgcggt ggtgctgccg gacgaactgg tgctgaac
//
LOCUS       DASWGM010000540         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1728004,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000540 DASWGM010000000
VERSION     DASWGM010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728004"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VGA15_15580"
     CDS             complement(<1..722)
                     /locus_tag="VGA15_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9589154.1"
                     /translation="MLSNDVTRYVAVYQALGLNFSEQSRTLHLYARYAEAYDDRFTLV
                     SRIRDWCSTASSPIRARSCYDTARRFCVFMNAEDSRHEVPPAGAFGRGKRPRPAPHLL
                     ELHQIRAIMNAALDLPPRGSIRPHTYYHLFGLLAATGLRISEALGLQRNDFTEDGLVV
                     RCGKFGKSRLLPIQASTRQALNDYIAIRTRLGCDGDDLFVVTTGRAPHKVTTHVIFVK
                     LARQLGLRGPGRTRGPRLHDLR"
     gene            complement(716..1891)
                     /locus_tag="VGA15_15585"
     CDS             complement(716..1891)
                     /locus_tag="VGA15_15585"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9589155.1"
                     /translation="MSLYADRLTSLGFCRGAWRIHYSAAEHFAAWLRLSRLRWDDVDD
                     AIVERFVRHTCRCSLCRKGAKLQESGAGKRRRGAHRFLTFLREQSAIPVTFMAPSEDL
                     HLSAYPTWLRQHCGVTDQTIARYVAEVSRWLPTLGAAYDAVAIRNVVLSQGPSRSRSS
                     VRLTATVLRSYLRFLVARGDCRPELYHAVPSVARRHLAPLPRYASAATIERIIASCEA
                     AATPVRLRDRAIILLLARLGLRAGDIRRLRLDDIDWSNALIYVHGKGRRAASLPLPQD
                     AGDAVLAYIEHGRPIAAQEYLFLRVLAPHTPLASSAEIAGIVSRVLARGGITGVPTGA
                     HMFRHSLATAMLRTGASLESVGTVLRHRSPGTTAIYAKVDIPMLMRVAQPWPGDASC"
     gene            complement(2629..>2851)
                     /locus_tag="VGA15_15590"
     CDS             complement(2629..>2851)
                     /locus_tag="VGA15_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HEX9589156.1"
                     /translation="DRTSDNAPSHRVYVGRAEIGAAWPKRSNEGRDYLSVKLDDPSFN
                     APIYANLFDDVDAETYTLIWSRSRKPNGD"
BASE COUNT          567 a          824 c          969 g          491 t
ORIGIN      
        1 tgccgcaagt catgaaggcg cggacctcgt gttcggcctg gcccccgcaa tccgagttgg
       61 cgggccaact tcacgaagat aacgtgagtg gtgaccttgt gaggagcgcg tccggtcgtg
      121 acaacgaaca gatcgtcacc gtcgcatccg aggcgggtac ggatcgctat atagtcgttc
      181 aaggcctgtc gcgtgcttgc ttggatcggc agcaggcggc tcttgccgaa cttgccacac
      241 cgcaccacca agccatcttc ggtgaaatcg ttccgctgga ggcccagcgc ctccgaaatg
      301 cgaagtcccg tcgcggcgag aaggccgaac agatggtagt aggtgtgcgg cctgatcgat
      361 cctctgggag gaagatcgag cgccgcgttc ataatcgcgc gtatctgatg cagttcaaga
      421 agatgcggtg caggccgcgg tcgcttgccg cggccaaacg caccggcggg cggaacttca
      481 tgccgggagt cctcggcatt catgaatacg cagaaacggc gcgccgtgtc gtagcatgat
      541 cgcgctctga ttggcgacga tgcggttgag caccaatcgc ggatccggct caccagcgtg
      601 aaccggtcgt catatgcctc ggcgtagcgg gcatagagat ggagggtccg gctctgctca
      661 ctgaagttaa gaccgagggc ttggtagacg gcgacatagc gggttacatc gttgctcagc
      721 atgacgcgtc tcccggccaa ggctgcgcga cccgcatgag catgggtata tcgaccttcg
      781 cgtagatggc ggtggtgcct ggcgatcgat gcctgagcac cgtgccgacc gactccaggc
      841 tggcgccggt ccgcagcatg gcggtcgcga gcgaatgacg gaacatatgg gcaccggtgg
      901 ggactccggt gatcccgcct cgcgcgagta cgcgggaaac gatgccggcg atctccgccg
      961 atgaggcgag cggggtgtga ggcgccagga cacgcaggaa caggtactct tgcgcagcga
     1021 tcggccggcc gtgctcaata taggcgagca ctgcgtcgcc agcgtcttgc ggtaatggca
     1081 ggctcgccgc ccgccgcccc ttgccatgaa cgtagatcag agcgttcgac caatcgatat
     1141 cgtcgagccg caagcgacga atatcgccgg cgcgaagccc gaggcgtgca agaagcagga
     1201 ttatggcacg atcgcgaagg cggaccgggg tcgccgccgc ctcgcatgac gcgatgatcc
     1261 gctcgatggt cgcggcgctc gcgtaccgcg gcagcggagc gagatggcgc cgcgcgaccg
     1321 atgggaccgc atggtagagt tcagggcgac aatctcctct tgccaccagg aaacgcagat
     1381 agctgcgcag cacggtcgcg gtcaggcgca cggacgagcg agagcggctg ggtccttggc
     1441 tcagcaccac gttgcggatt gcgactgcgt cataggcggc gccgagcgtc ggcaaccatc
     1501 gggacacctc ggcgacgtat ctggcgatcg tctgatcggt gacaccacaa tgctgtcgaa
     1561 gccacgttgg atacgctgat aggtgaagat cctcggatgg cgccatgaag gtaaccggga
     1621 tggcgctttg ctctctcagg aacgtgagaa accgatgcgc gccacgccga cgcttgcccg
     1681 cgccagactc ctgcagtttg gcgcccttgc gacaaaggga gcagcgacag gtgtgccgaa
     1741 cgaagcgctc gacgatggcg tcatcgacat catcccaccg gagtcgcgag agccgaagcc
     1801 atgctgcaaa gtgctcggcg gctgaataat ggatgcgcca agcgccgcgg caaaagccca
     1861 aggaggtcag gcggtcggca tagagcgaca aataggggcg gacatcctcg acgccatccg
     1921 tgacgggaat atcgccgcgg tcttcgagga agcggacgaa ccgccccaca cggccgagat
     1981 agaccggatt gcgaagctgc gccggactat aacgcggaca gcggcattgg tgcttcgcga
     2041 accgagcgac ggttgtagca cccacgctgg cgagcgggag gcggcgacga tcgagccagt
     2101 gtaggaaatg acgggcggca ctgccgtaat ttatgaacga agcggtgccg cgcaagggct
     2161 cgagaaatgc ggtgaccgca tcggtcgaag aaggcgcgtc gtctgcaaga cgaggtcgaa
     2221 agagtaaatg gcgcgggttg aatatcatgt tgatccctcc gggttgtggc aaccgaaggg
     2281 accataatta tgcggagcgg atcacgcggc ttcgcgttgg aaagtgggct gctccacaag
     2341 tctgctcggc ataatccgct gctcggcata aagcatctta tgctgagatc ggcataagat
     2401 gctttctggc ggccgacctt cctcatgcaa gacggtctcg atgatcttcg ctgtctttga
     2461 tttggagaac cccgcgtttg cgcaggaagc tgtcgcggtc ctcgtcgcag cggggtctgg
     2521 tgcaaggtac cagcagacgc gcaggagcgc gtcgcaaaca ctgcttggcg gcaacgcaat
     2581 ggcgaaacgc cccgctcgat gagcggggcg ttgcactcgg ggcctggttc agtcgccgtt
     2641 gggcttgcgg ctgcgggacc agatcagggt gtaggtctcg gcgtctacgt cgtcgaagag
     2701 gttggcgtag atcggagcgt tgaagctcgg atcgtccagc ttgaccgaga gataatcgcg
     2761 gccctcgttg gagcgcttgg gccaggcggc tccgatctca gcacgaccca cgtagacccg
     2821 gtggctcggc gcattgtcgc tggtccggtc t
//
LOCUS       DASWGM010000541         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2558561,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000541 DASWGM010000000
VERSION     DASWGM010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2558561"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..633
                     /locus_tag="VGA15_15595"
     CDS             325..633
                     /locus_tag="VGA15_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589157.1"
                     /translation="MKETTRLRQLEAECRQRALSEPQRKWYWLAQAAKFQIQAVQEIA
                     FHREDCNITDSPEVKLAQWPNGPDGRRLMEPFVREGGQLDDFYRPLKDRRRESGLTPF
                     "
     gene            complement(620..1498)
                     /locus_tag="VGA15_15600"
     CDS             complement(620..1498)
                     /locus_tag="VGA15_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9589158.1"
                     /translation="MSEWIGVAIALVSSCLCGSAAAITRYLVGNADPITLAILRWGIG
                     FACLLPTALLLHVRWPPRRDWLAVAALGLCFFGLFFVLYNIAIGFTTAARASLALATL
                     PLHTMVIAALLGIEPLTLRKLAGVCVAVLGVMAALAYGLSAAPPGAWRGELIMTGAVL
                     CMAFYNVWSRPFIQRSSALGFLTVGMGAGAAALILVGALTGSIAVLRGFGASQWIAGI
                     YLGVGGGALAFILWVMALERATPTRVASTMTVNPIAAGLLATQLVGEPITANLVAGLG
                     AVFAGIWIATTEVKKA"
     gene            complement(1808..2893)
                     /locus_tag="VGA15_15605"
     CDS             complement(1808..2893)
                     /locus_tag="VGA15_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9589159.1"
                     /translation="MQQIEVAVIGTGWCGGIRAETLSRSSIVDKLHICEIRPDRLAEV
                     KQLTNAATATSDYHDIIKNDRIKVVYISTTPESNHYPIARDCLKAGKHVLLEKPIALE
                     LFEADELIEIAKRGNLKFTIGYSQRFNTKLAYARKKIADGTLGKPVSVMVSRHLSRGL
                     GKKIASRGRLSPATMESTHDLDFVFWLLEPARPIRVYSQGAYGYMEAVNGSYDIMWST
                     VTMDNGVLVSIGGGWNLPPSYPNYSATTIEITGTEGSLFLDDTQRSNWLNTMKEGTQY
                     PMSTMPGEWVDHVYAGQMGPETMHFLECVLRDQPPMVSAESARRVMEAYLAADLSAES
                     GEPVSLPLNNQALSAVQELYKKFRTPV"
     gene            complement(3014..>3359)
                     /locus_tag="VGA15_15610"
     CDS             complement(3014..>3359)
                     /locus_tag="VGA15_15610"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9589160.1"
                     /translation="QFVKEKQVKALAVTTRERMADLPDIPTMYELGWKNLVTSSWQGV
                     LVPSGTPRPIVDKLHAAIVKVLAEPEVQARMRKGGVIAVGSKSPEDFKTYMDVETAKW
                     TKVIEESGLHPD"
BASE COUNT          654 a         1049 c         1022 g          634 t
ORIGIN      
        1 gagccgaccg agccgtttct cggaacggtt cgcctggaca aactccccta tgagaacgtc
       61 gacctgaagc agtattaccc ctacggaaac ccggcgtttc ccggccaagg cttaagcgcc
      121 gcaacgcgtc gggtcgatga cttggcgcct tgatcacagc gcgcaaattg tcggattgat
      181 ctatccatat ttcaataaat tgcgtcgtac ctccctagtc cgccgctaag cacagttttt
      241 gtcttggaga aacgatcgcc ggaaaccaat ttgccggtga gtcagggcga agtatgcatg
      301 aacttaagag ctgtggagtt tgatatgaaa gaaaccaccc gcttacgcca gttggaagcc
      361 gagtgccgcc agcgcgcgct aagcgaaccc caaagaaaat ggtactggct cgcacaggcc
      421 gcgaaattcc agatccaggc tgtccaggaa atcgcttttc accgtgaaga ctgcaacatc
      481 accgactctc cggaagtcaa actggctcaa tggccgaatg gtcccgatgg aagacggctg
      541 atggagcctt tcgtccgtga aggcggacag cttgacgatt tttatcgccc gttaaaggac
      601 cgccggcgag aatcggggct tacgcctttt tgacctccgt cgtcgcaatc catatcccgg
      661 caaagacggc gccgaggccg gcgacgagat tggccgtgat cggctcgccg accagttgcg
      721 tagcaagcaa tcccgccgcg atcggattga cggtcatggt gctggcgacc cgggttggcg
      781 tcgctcgctc cagcgccatg acccacagga tgaaggcgag cgcgccgcca ccgacgccga
      841 gatagatacc ggcaatccat tgcgaggcgc cgaagccgcg taacaccgcg atgctgccag
      901 tcagcgctcc caccaggatc agtgccgcgg cgccggcgcc catgccgacg gtcaggaaac
      961 cgagtgcgct cgatcgctgg atgaatggac gggaccagac gttgtagaac gccatgcaca
     1021 gcaccgcgcc ggtcatgatg agctcgccgc gccacgcacc cggtggcgcc gccgaaaggc
     1081 cgtatgcgag tgcggccatg acgccgagca cggcgacgca gacgccggcc aatttccgca
     1141 aggtcagcgg ctcgatcccg agtagtgctg cgatcaccat ggtgtgcagc ggcaacgtcg
     1201 ccagcgcgag gctggcgcga gccgcggtcg taaagccgat cgcgatgtta tagaggacga
     1261 aaaacagtcc gaagaaacac agccccagcg ctgccacggc gagccagtcg cgacgcggcg
     1321 gccatcgcac atgcaacaaa agcgccgtcg gcagcaaaca tgcaaacccg atgccccagc
     1381 gcagaatggc aagcgtgatc ggatcggcat tgccgaccag atagcgcgta atcgccgccg
     1441 cgctgccgca gaggcagcta gagaccaatg cgatcgccac tccgatccat tcactcaccg
     1501 cgcgcccccc aagccatcgc atataacatg aatgaatgag ccacgcgttg atgcggtcgc
     1561 ggcttcgcac gagctatcag cgggtcgtcc ctgcgaacgt tcgcagggac gacccattga
     1621 acgccttctt gctgctacgc tgtcaagacg tggatgcccg ggacgtctat cgcgaagacg
     1681 cgcttcgcgc ttcgcgacgc ttggtattcc gcaggacatc acgccgcagg aactgcgcgt
     1741 cgaatgcgtt gggctttgcg gatggttcat agccctgagg tgcgaacgcg ggcggcgccg
     1801 cgactggcta gaccggcgtg cggaacttct tgtagagctc ctgaaccgcg gagagcgcct
     1861 ggttgttgag gggaaggctg acgggctcgc cggattccgc cgagagatcc gcggcaagat
     1921 aggcctccat cacgcggcgc gcgctttccg ccgacaccat cggcggctgg tcccgcaata
     1981 cgcactcgag gaagtgcatc gtctcgggtc ccatctgacc ggcgtagacg tgatcgaccc
     2041 actcgcccgg catggtcgac atcggatatt gcgtcccctc cttcatcgta ttgagccagt
     2101 tggagcgctg cgtatcgtcc aggaacaggg agccttccgt gccggtgatc tcgatcgtcg
     2161 tggcggagta gttcggatag cttggcggca ggttccagcc gccgccgatc gaaactagca
     2221 cgccattgtc catggtcacg gtgctccaca tgatgtcata ggagccgttg accgcctcca
     2281 tgtagccgta ggcgccctgc gaatagacgc ggatgggcct ggccggctcg agcagccaga
     2341 acacgaaatc gaggtcgtgg gtcgattcca tcgtcgccgg cgacagccgg ccgcggcttg
     2401 cgatcttctt gccgaggccg cgcgacagat gccggctcac cattaccgac accggcttgc
     2461 ccagcgtgcc gtcggcgatc ttcttcctgg cgtaggcgag cttggtgttg aaacgctgcg
     2521 agtagccgat ggtgaatttg agattgccgc gtttggcgat ctcgatcagt tcgtcggcct
     2581 cgaacagttc gagcgcgatc ggcttttcca agagcacgtg cttgccggcc ttgaggcaat
     2641 cgcgcgcgat cggatagtgg ttggattccg gcgtcgttga gatgtagacc accttgatcc
     2701 ggtcgttctt gattatgtcg tgatagtctg atgtcgcggt tgccgcgttg gtcagctgct
     2761 tgacctcggc caggcggtcg ggcctgattt cgcagatgtg aagcttgtcg acgatcgaag
     2821 agcgcgacag cgtttccgcc cgaatgcccc cgcaccagcc agttccgatc accgcgactt
     2881 cgatctgttg cacgcttgtg tctcctgttt tccactatcg aatgccggcg aatatgtctt
     2941 cggcaatgcc aatactcggt gccgcgcaga gaaggaccat tcacccgcgc cgcgtcaacc
     3001 ggaacgcggc ggctcaatcc ggatgcagcc cgctctcttc gatcaccttg gtccacttgg
     3061 cggtttcgac gtccatgtag gtcttgaagt cctcagggga cttgctgccg acggcgatca
     3121 cgccgccctt gcgcatgcgc gcctgaacct ccggctcggc gagcaccttg acaatcgcgg
     3181 cgtgcaactt gtcgacgatc ggccgcggcg tgcctgatgg cacgagcacg ccctgccagg
     3241 atgacgtcac gaggttcttc catccgagct cgtacatggt cggtatgtcc ggcagatcgg
     3301 ccattcgctc ccgggtggtg acggcgagcg ccttgacctg cttctccttc acgaactgc
//
LOCUS       DASWGM010000542         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_894194,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000542 DASWGM010000000
VERSION     DASWGM010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894194"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VGA15_15615"
     CDS             complement(<1..659)
                     /locus_tag="VGA15_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9589161.1"
                     /translation="MARIRIGLAGCGFVSELHMYACRRVYGMDVEVKAVAARGEHVLD
                     FARRHQIPRAYRSFRDLIADGELDVVDICTPPNLHTSMIVDAMQAGKHVICEKPFAGY
                     FGREGDKVPIGKHVPKALMYERVLEEMESTRAAIGKTGRLFMYAEDWIYAPAVTKTVE
                     ILKATKDKILFMKGEESHSGSHAAHAAQWAMTGGGSLIRMGCHPLAAVLYLKQVEAKA
                     RG"
     gene            1054..>1802
                     /locus_tag="VGA15_15620"
     CDS             1054..>1802
                     /locus_tag="VGA15_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589162.1"
                     /translation="MRVSIITGGGSGIGAAIAHRLAARDECLMLHGQGADAAGLERLK
                     AVAADCERAGAKVAWCTGDLADAGTASELVRAARAAFGSVDAIVHAAGFADRRGFTQL
                     PRASLERSFAVMASAFHELAASALPDLTRGSQGRVVAISSFAAHRFTLEATYPGSAAA
                     KAALEVLVRCLAIELASSGATANVVMPGYTRKDAGLLGALDPQTWQLVAKANPLARLA
                     EPDQVAAVVAFLLSPEAGHVTGALLPVDGGLT"
BASE COUNT          307 a          562 c          566 g          367 t
ORIGIN      
        1 ccgcgtgcct tcgcctcgac ctgcttgaga tagagcacgg ccgcaagcgg atggcatccc
       61 atccggatca atgaaccgcc gccggtcatg gcccattgcg ccgcgtgtgc cgcatgcgag
      121 ccgctgtgac tctcttcacc cttcatgaac aggatcttgt ccttggtggc cttcaggatc
      181 tccacggtct tggtcactgc cggcgcgtag atccagtctt ctgcatacat gaagagcctg
      241 ccggtcttgc cgatcgccgc gcgggtgctt tccatttctt ccagtacgcg ctcgtacatc
      301 agggctttgg gcacgtgctt gccgattggc accttgtcac cttctcgccc gaaataaccc
      361 gcaaacggct tttcacagat gacatgtttg ccggcctgca tcgcatcgac gatcatcgac
      421 gtgtgcaagt tgggcggcgt gcagatgtcg acaacatcga gttcgccgtc ggcgatcaag
      481 tcgcgaaagc tgcggtatgc cctcggtatc tggtgccgcc gcgcaaaatc gagaacatgc
      541 tctcccctcg ccgcgacggc tttgacctcg acgtccattc cgtagacacg cctgcaggca
      601 tacatgtgca gctccgaaac gaagccgcag ccggccagac cgatcctgat ccttgccatt
      661 gcggatgcct ccagtggtcg tcggtcaata gcgaagcggg acggcggccg cggatgagcc
      721 ggcgatgatt cgacgggata tcggctctcg gaccggagct gacgggcgtc ctttagcagt
      781 tttccgtttt gcgcccaatg gtagaaatac gcacgcgata ccgacgcaaa tctgcagacc
      841 tgcgcttgat ccctcggtcg aacctcccat cagagtcagg ttttgaaccg actgagagat
      901 gggaagacgc cgacgacctc tacgtcggct cgcatcggtt gcgccgcttc gctgcaatcg
      961 ctgcgattgc tcccgagacc gttgcgattg ttggcatttg caattgcggc acctatagtt
     1021 tgcgcgatca agagcgttca agaaccagca tccatgcgcg tttcaatcat cactggaggc
     1081 gggtccggga tcggagccgc gatcgcgcac cggttggcag cccgggacga gtgcctgatg
     1141 ttgcatggac aaggggccga cgcggcaggg ctcgagcgct tgaaagcagt ggccgcggac
     1201 tgtgagcgcg ccggcgccaa ggtcgcgtgg tgcaccggtg acctggcaga tgccgggacg
     1261 gcatccgagc ttgttcgcgc ggcgcgcgct gcgttcggaa gcgtcgacgc gatcgtccat
     1321 gccgccgggt ttgcggatcg gcgcggtttc acgcagttgc cgcgcgcgtc gctcgaacgg
     1381 tcattcgcgg tcatggcatc agcgtttcac gaattggcgg cctccgcgtt gccggacctg
     1441 acgcgcggat cgcaaggacg cgtcgtcgcc atctctagtt tcgctgccca tcggttcacg
     1501 cttgaggcga cctatccggg gtctgcggcg gccaaggccg cgctcgaggt gctggtccgg
     1561 tgtcttgcaa tcgagctcgc aagctcaggc gcaacggcca atgtcgtcat gcccggctat
     1621 acgcgcaagg atgccgggct attgggggca ttggacccgc agacctggca attggtcgcc
     1681 aaggccaatc cgctcgcacg attggccgaa cccgaccaag tagccgcggt ggtcgccttt
     1741 ctgttgtcgc cggaggctgg acatgtgacg ggagcgctgt tgccggtcga tggcggcctg
     1801 ac
//
LOCUS       DASWGM010000543         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1155663,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000543 DASWGM010000000
VERSION     DASWGM010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155663"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VGA15_15625"
     CDS             <1..280
                     /locus_tag="VGA15_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9589163.1"
                     /translation="ALYSMSSAAKMTSFVPISGVPVQLLGGRRWLAVLGSVGQPRDRD
                     PAASFAMFDTNSREISYCRVPYDTEAAASRIRDNGLPLWLADRLSVGR"
     gene            284..>1552
                     /locus_tag="VGA15_15630"
     CDS             284..>1552
                     /locus_tag="VGA15_15630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX9589164.1"
                     /translation="MAKPSVKTSVKLSVVSGAVIDGFTIGECVHHGGMATLWSVTRPD
                     IAMPLLMKIPLVSEGEDPAAIVSFEMEQMILPRLSGPHVPACFGTGDFASQPYVVIER
                     IPGKTLYGRLGDLPLSFEEASGIVGKIALALADLHRQNVIHHDIKPSSIMYRPSGEVV
                     LIDFGLSHHNQLPDLLQEEFRLPYGTAPYMAPERLLGVRDDPRSDLFSLGVLLYFFTT
                     GVRPFGESETMRGMRRRLWRDPHPPRKLKADYPPWLQEVVLRCLEIEPVWRYPTASQL
                     AFDLGNPDQIKLTARSERVKRDPISTVWRRRFNRGLTRPKPKSDLAAQLAACPILAVA
                     LDTAEGSEPLNEALRVTVQRILATLPSARLACLNVLKLGRVTIDRTLDEQGNNKHIDR
                     LVTLKHWASPLKLDENRLTVHVLEAIDPAAA"
BASE COUNT          271 a          484 c          495 g          302 t
ORIGIN      
        1 ggcgctctat tcgatgtctt cagccgcgaa gatgacgagc ttcgtcccga tatcgggcgt
       61 tcccgtgcaa ttgctcgggg gaaggcgctg gctggccgtt ctcggctcgg tgggccaacc
      121 ccgcgatcgc gatccggcag cttcgtttgc gatgttcgat accaactccc gggaaatcag
      181 ctattgccgt gtgccctacg ataccgaggc ggcggcgagc cggattcgcg acaacggcct
      241 gccgctctgg ctggccgatc ggctctcggt cgggaggtga gcgatggcca agccttcggt
      301 taagacttcg gttaagctct cggttgtatc aggcgccgtc atcgatgggt tcacgatcgg
      361 ggaatgcgtc catcacggcg gcatggcgac gctgtggagc gtgacccgcc ccgacatcgc
      421 gatgccgctg ttgatgaaaa ttccgctggt gtccgagggt gaggacccgg ctgccatcgt
      481 cagtttcgag atggagcaga tgatcctgcc gcggctgtcg gggcctcatg tgccggcctg
      541 tttcggcacc ggcgatttcg caagccagcc ttatgtcgtg atcgagcgca ttcccggcaa
      601 gacgctttac ggcaggcttg gcgatctgcc gctgagcttt gaggaggcga gcggcattgt
      661 cggaaaaatc gcgttggcgc tggccgacct gcatcgacag aacgtcatcc accacgacat
      721 caagccgagc agcatcatgt atcgcccctc gggcgaggtg gtcctgatcg attttggctt
      781 gtcgcatcac aaccagttgc ccgatctgct gcaggaagaa ttccggctgc cctacggcac
      841 ggcgccgtat atggcaccgg agcgtttgct tggcgtgcgg gacgatccgc gcagcgacct
      901 tttttcgttg ggcgtgcttt tgtacttctt caccaccggc gtgcggccgt tcggcgagag
      961 cgagaccatg cgcggaatgc ggcggcggct gtggcgcgat ccgcatccgc cgcgcaaact
     1021 gaaagccgac tatccgccct ggctgcagga agtcgtgctg cggtgcctcg aaatcgagcc
     1081 ggtttggcgc taccccacgg catcgcaact ggcattcgac ctcggtaacc ccgaccagat
     1141 caaattgacc gcgcgatcgg agcgggtgaa gcgcgatccg atcagcaccg tgtggcgccg
     1201 ccgtttcaat cgcggcctga cgcggccgaa accgaaatcc gatttggcgg cgcagctggc
     1261 tgcgtgtccg attctggcag tcgcgctcga taccgcggag ggatctgagc cactgaacga
     1321 ggccctgcgc gtcaccgtcc agcgaatcct ggcgaccctg ccatccgcaa gactcgcctg
     1381 cctgaatgtc ttgaaacttg gccgcgtcac catcgacagg acgctcgacg agcagggcaa
     1441 caacaagcac atcgaccggc tggtcacgct caagcattgg gcgtcgccgt taaagctcga
     1501 tgaaaaccgg ctcacggtgc acgtcctcga ggccatcgat cccgcggcgg cg
//
LOCUS       DASWGM010000544         5178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2558850,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000544 DASWGM010000000
VERSION     DASWGM010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2558850"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VGA15_15635"
     CDS             complement(<1..573)
                     /locus_tag="VGA15_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEX9589165.1"
                     /translation="MDFNINNLATPGIWIGVLVAILILLRVANIFRYISNNQVGIVEK
                     MWAAKGSIQSGFIALHGEAGFEPEILRGGVHVFFPFTYRIHTSDLVTVGQGKIAYVFA
                     RDGASLGASQVLAANDTEDRSDFQDVRRFLLGGGQKGPQRKVLREGTYAINTTQFAII
                     TDERVYGHALSQQEQGVLDSMKQTIADRDGF"
     gene            complement(778..2103)
                     /locus_tag="VGA15_15640"
     CDS             complement(778..2103)
                     /locus_tag="VGA15_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX9589166.1"
                     /translation="MAAAITTDARAGAARPWYKILYVQVLIAILLGVVVGWLAPTIAT
                     NDWIKALGDGFIKLIKMVIAPIIFCTVVSGISHIQDARKVGRVGVKALVYFEIVSSLA
                     LIIGLVVGNLIPVGRGLAVNPDAAAVATYVKQAEAQKSVDFVLNIIPDSVVGALARGD
                     ILQVLLFAILFGFALMALGERGHRLREIIDDTAHAVFGVIGIVMKAAPIGAFGAIAYT
                     IGKFGPAALGNLVGLIAVFYMTAALFVFGVLGLIARLVGFNIFKFLAYIKDELLLVLG
                     TSSSESALPQLMAKLERLGCSKPVVGLVVPTGYSFNLDGTNIYMTLATLFIAQALGVD
                     LSFSQQLTILIVAMLTSKGASGVTGAGFITLAATLTVVNPALVPGMAIVFSVDKFMSE
                     VRALTNIIGNGVATVFVSWWEGELDHDRLHANLNKEIDPSDVETAVTTG"
     gene            complement(2231..2734)
                     /locus_tag="VGA15_15645"
     CDS             complement(2231..2734)
                     /locus_tag="VGA15_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX9589167.1"
                     /translation="MALGTALLTLSAKVNLPLPYVPMTLQTLVVLMIGAAYGARLGGA
                     TVAAYLAMGAIGWPVFAGPIGGLAPLAGPTAGYLFGFVAAAFITGWLSERGWDRSVPR
                     LFVAMGLGHIVILAAGFAWLAFGMKLGIDKAWLVGIAPFFAASVIKNALGAALVPAIR
                     HVLDRRG"
     gene            complement(2942..4129)
                     /locus_tag="VGA15_15650"
     CDS             complement(2942..4129)
                     /locus_tag="VGA15_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9589168.1"
                     /translation="MLDATLRERASGLLTASGRSNVPPFMVMDVMAAAARIEAAGGHV
                     IHMEVGQPAASAPKTAISAAHAALDGGRIDYTSALGMPSLRDRIARHYRETYGCAVDA
                     ERIVVTTGSSGAFILAFLALFEPGDRVAVTVPGYPPYRHILTALGCEPVLIETSSENR
                     HALIGEALLAAHSKNPLKGVLVGSPANPTGTMMSRDALTSLIAAAEGAGIRFISDEIY
                     HGLDYAFSAVTAAELSRDALVINSFSKYFCMTGWRVGWMVVPEPLVRPIERLQQNLSI
                     SVPTLSQIAAEAAFEGRLEMEEVKRGYLENRDILIAGLPQAGLTKFLPADGAFYLYAD
                     VSEFTSDSFKFANEMLEKAHVAATPGIDFDPVHGHRFIRFSYARSADEMREAVTRIAR
                     WLG"
     gene            4240..>5178
                     /locus_tag="VGA15_15655"
     CDS             4240..>5178
                     /locus_tag="VGA15_15655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9589169.1"
                     /translation="MLLRMALRKKMARLTAVTTAVALAIAPVPALTQQQKGPPVIRDT
                     ESEQLLREYTRPILRAAGLEKQNIQIVIINESVFNAFVADGRRIFVNYGAMMQSETPN
                     QIIGVLAHETGHLAGGHLAKMREQMAQAQTQMIIAMLLGAGAMIAGSRGNNSNGGLAN
                     AGAAAVAGPQEMIRRNLISYVRQQEENADRAGVKFLTATGQSAKGMYETFKRFTNDSL
                     FASHGADPYVQSHPMPAERVAALEEIARSSPYWEKKDDPTLQLRHDMVRAKISAFMER
                     QDTVYRRYPMSNDSLPARYAHAISTYLHGDLRSALAQ"
BASE COUNT         1059 a         1652 c         1558 g          909 t
ORIGIN      
        1 gaaaccgtcg cgatccgcaa tcgtctgctt catgctgtcg agaacgccct gttcctgttg
       61 actcaaggcg tgaccataga cccgctcgtc ggtgatgatt gcaaattgcg tggtgttgat
      121 cgcgtaggtt ccctcgcgca gaaccttgcg ctgcggacct ttctggccac cgcctaggag
      181 aaatctccga acatcctgaa agtcggacct gtcctcggtg tcgttggccg cgagtacctg
      241 ggaggccccc agggaagccc cgtcgcgcgc gaacacgtag gcgatcttgc cctgtccgac
      301 cgtgacgagg tcggaggtat ggattcgata ggtgaaggga aaaaatacgt gaacgccgcc
      361 gcgcaggatc tcgggctcga acccggcctc accatggagc gcgatgaagc ccgattggat
      421 tgaacctttc gcagcccaca ttttctccac gatcccgact tggttgttcg agatgtagcg
      481 aaagatattg gccacccgca gcaaaataag tatcgcgacc aagaccccga tccagatgcc
      541 gggtgtagcg aggttattga tgttgaagtc catgcttgaa gctccccagg tgcctgtatc
      601 tgcctgcgta aaggacgcag atgctgtcgg tttggcggca gcgatgtctc cgaatttggc
      661 cgtcgttgcg tcgcagtggt tcgcctgcgc ctggacgcct tgaattgagg atgttgtatt
      721 tccccggcca ggctgggtgc gatcttttga ctaccccttg atgatttcac gcccaagcta
      781 accggttgtg acagccgttt ccacgtcgga gggatcgatc tctttattga gattggcatg
      841 cagcctgtcg tgatcgagct cgccctccca ccacgacacg aacaccgtgg cgacgccatt
      901 gccgatgatg ttggtgagcg cgcggacctc gctcatgaac ttgtccaccg aaaacacgat
      961 cgccatgccc ggcaccagcg ccggattgac caccgtgagc gttgccgcca gcgtgatgaa
     1021 gccggcgccg gtgaccccgc tggcgccctt cgacgtcagc atggcgacga tcaggatggt
     1081 caactgctgg ctgaacgaca ggtcgacgcc gagcgcctgc gcgataaaca aggtcgccag
     1141 cgtcatatag atgttggtgc cgtccagatt gaacgaatat ccggtcggca ccaccagccc
     1201 gaccaccggc ttggaacagc ccagacgctc gagcttcgcc atcaactgcg gcagcgcgct
     1261 ttccgacgaa ctggtgccga gcacgagcag caattcgtcc ttgatgtagg cgaggaactt
     1321 gaaaatatta aagccaacga gccgcgcaat cagcccgagc acgccgaata cgaacagcgc
     1381 cgcggtcatg tagaacaccg cgatcagtcc gaccagattg ccgagtgcgg cgggtccgaa
     1441 tttcccgatg gtgtaggcga tcgcaccaaa ggcgccgatc ggcgccgcct tcatgacgat
     1501 gccgatcacg ccaaacaccg catgcgccgt gtcgtcgatg atctcgcgca gccgatgacc
     1561 gcgttcgccg agcgccatca gcgcaaagcc gaacaggatc gcgaacagca gcacctgcag
     1621 gatgtcgccg cgcgccagcg cgccgaccac gctgtccgga atgatgttga gcacgaaatc
     1681 gaccgacttc tgcgcttccg cctgcttgac gtaggtcgca accgctgccg catccgggtt
     1741 cacggcgagc ccgcggccca ccggaatcag attgccgacc acgagaccga tgatcagcgc
     1801 caacgatgag acgatttcga aatagaccag cgccttgacc ccgacacggc cgactttcct
     1861 ggcatcctga atatgcgaaa tgcccgacac gacggtgcag aagatgatcg gcgcgatcac
     1921 catcttgatc agtttgatga agccgtcgcc cagcgccttg atccagtcat tggtcgcgat
     1981 agtgggcgcc agccagccga cgacgacacc gagcaggatc gcaatcagca cctgcacgta
     2041 cagtattttg taccagggtc tcgccgcgcc cgcccgcgca tcggttgtga tcgctgccgc
     2101 catgaccttc cctccccgtc agaacttgcg gatctgtctg tcgttgttcg cagacagctt
     2161 tcccaccgga tacaaccaag atcgaatgac tgagcggagt caatggcgtt gccttcgacc
     2221 gccggcggtt ttagccgcgg cggtcgagca catgcctgat cgccggcaca agggctgctc
     2281 cgagtgcgtt cttgatcacg gacgcggcga agaacggcgc gatcccgacc agccatgcct
     2341 tgtcgatgcc gagcttcatg ccaaacgcga gccatgcaaa tccggccgca agaatcacga
     2401 tgtggccaag tcccatcgcc acgaaaagcc gcggcacgga gcgatcccag ccgcgctcgc
     2461 tcagccatcc cgtgatgaag gccgcggcga cgaaaccgaa tagatagccg gcggtcggtc
     2521 ccgccagcgg cgcgaggccg ccgatcggac ctgcgaacac cggccatccg atggccccca
     2581 tcgccagata ggccgcgacg gttgcgccgc cgagccgcgc gccataggcg gcgccgatca
     2641 tcagcacgac cagcgtttgc agcgtcatcg gcacgtaggg cagcggcaga ttgaccttgg
     2701 ccgagagcgt cagcagcgct gttcccaagg ccatcaggat cacgatacgc agcacaccgg
     2761 aaaccccttc ggcgcgatcg ggccacagca gctcagcgag cggaaagtgc ggcgtgacag
     2821 cgggctctgg cattgatctc gtggacaagg acatctcctg atatgggcaa gttgctcggc
     2881 gcaggtgaag attagcaggt gaagattagg tgaagagagg aatcgaaagt gcttatggac
     2941 cttatccgag ccaccgcgcg atgcgcgtca cggcttcgcg catctcgtca gcggagcgcg
     3001 cataggaaaa gcggatgaac cggtggccat gaaccggatc gaagtcgatc ccgggggttg
     3061 ctgccacatg cgctttctcc agcatctcgt tggcgaactt gaaactgtcg gaggtgaact
     3121 ccgatacatc cgcgtagaga taaaacgccc cgtcggcggg caggaattta gtgaggccgg
     3181 cttgcggcag gccggcgatc aagatgtcgc ggttctccag gtagccgcgt ttgacttctt
     3241 ccatctccag acggccctcg aatgccgcct ctgcggcaat ctgcgacagc gtcgggaccg
     3301 aaatcgagag attttgctgc agccgttcga tcggccgcac cagcggttcc ggcaccacca
     3361 tccagccgac ccgccagccg gtcatgcaga aatatttcga gaacgaattg atgacgagcg
     3421 catcgcgaga aagctccgcc gccgtcaccg ctgaaaacgc gtagtcgagg ccatgataaa
     3481 tctcgtcgga gatgaaccgg atacccgcgc cttccgcggc agcgatcagg ctggtgagcg
     3541 cgtcgcggga catcatcgtg cccgtgggat tggccgggct gccgaccagc acgcctttca
     3601 gcggattttt gctgtgggcg gcgagcaagg cttcaccgat cagcgcgtga cggttttcgc
     3661 tcgatgtttc gatcaacacc ggctcgcagc cgagcgcggt gaggatatgg cgatatggcg
     3721 gataaccggg aaccgtgacc gcaacgcgat cgcccggctc gaacaaggcg agaaaggcca
     3781 ggatgaacgc gcccgaggaa cccgtggtga ccacgatccg ctcggcatcc acggcgcagc
     3841 cgtacgtctc acggtagtgc ctggcgatgc ggtcgcgcag cgagggcatg cccagcgccg
     3901 aggtgtagtc gattcggccg ccatcgagcg ccgcgtgagc ggccgatatc gcggtcttcg
     3961 gcgccgaagc cgccggttgt cccacttcca tgtgaataac atggccgccg gccgcttcaa
     4021 tgcgcgccgc cgccgccatc acgtccatca ccatgaacgg cggaacattg ctgcgccccg
     4081 acgccgtcag caggccgctg gcccgttctc tcaatgtcgc atccagcatc gatatctgct
     4141 atttatcccg gcgtctcggt gccccagact agccgcattc cgcagcttgt tagggcataa
     4201 cccgtatccg gcccgccgcc aacgactaaa gattgctcaa tgttgctccg catggcgcta
     4261 cgcaaaaaaa tggccaggct gaccgctgtc accaccgcgg tcgcgctcgc cattgcgccg
     4321 gtgcccgcgc tgacgcagca gcaaaagggc ccgccggtca tccgcgatac cgagtccgag
     4381 caactgctgc gcgaatacac gcggccgatc ctgcgggccg cgggcctgga aaagcagaat
     4441 atccagatcg tgattatcaa cgagagcgtt ttcaacgcct tcgtcgccga tggccgccgc
     4501 attttcgtga actacggcgc gatgatgcaa tcggagaccc cgaaccagat catcggggtg
     4561 ctggcgcatg aaaccggcca tctcgccggc ggccatctcg ccaagatgcg cgagcagatg
     4621 gcgcaggcgc agacccagat gatcatcgcc atgttgctcg gcgccggcgc gatgatcgcg
     4681 ggctcgagag gcaacaacag caatggcggc ctggccaatg ccggcgcggc cgcggtcgca
     4741 ggaccgcagg aaatgatccg ccgcaacctg atctcctatg tgcgccagca ggaggaaaat
     4801 gccgaccgcg ccggggtgaa gtttctgacc gcgaccgggc agtccgccaa gggcatgtac
     4861 gaaaccttca agcggtttac caatgacagc ctgttcgcct cgcatggtgc ggatccatac
     4921 gtccagtcgc atccgatgcc cgccgagcgc gtcgcggcac tggaggaaat cgcgcggtcc
     4981 agtccctatt gggaaaagaa agacgatccg accctgcagc tgcgccacga catggtgcgc
     5041 gccaagatct cggccttcat ggagcggcag gacaccgtct accggcgcta tccgatgtcg
     5101 aacgacagcc tgccggcgcg ctatgcgcac gcgatctcga cttacctgca cggcgatttg
     5161 cgctccgcgc tcgcccag
//
LOCUS       DASWGM010000545         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1008178,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000545 DASWGM010000000
VERSION     DASWGM010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008178"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..120
                     /locus_tag="VGA15_15660"
     tRNA            45..120
                     /locus_tag="VGA15_15660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Arg,seq:gcg)
     gene            complement(139..828)
                     /locus_tag="VGA15_15665"
     CDS             complement(139..828)
                     /locus_tag="VGA15_15665"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX9589170.1"
                     /translation="MFIDAGHGGLDPGVVGATAAGHQVLEKDATLAVASRLAAVLRAD
                     GYSVVMSRTRDTTVIKLAAADSNYGAITSSAVHRDLAARAACANAAGANVLISIHFDG
                     FSDPSVGGTETFYDAARPFAAANHRLAADLQSALVARLGVADRGIFTDDQLAAPALTA
                     SGDSYNHLIELGPQSPGWVDNPSQMPGALVEPLFITNPSEAQIASDPAGQQKIAEALA
                     AGVLRFESAPA"
     gene            complement(1141..>1648)
                     /locus_tag="VGA15_15670"
     CDS             complement(1141..>1648)
                     /locus_tag="VGA15_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589171.1"
                     /translation="RCNLSGDINDYLKKIQTKDPITYSTIKGQWAAAQKDGATAADVE
                     FYTDSAAHCTALESNGSQIGTATYKLVVNFVFQFKDQASAEKGYSTDSIFGFSAPTLK
                     ASQVPVTEGTATGLGKNSIVLSISISNQAFYVAVWQNKQFMVILGILNIDTGADKKIA
                     LAENGRIH"
BASE COUNT          296 a          543 c          520 g          289 t
ORIGIN      
        1 ccgccacggc agcggtcgat gctcgcctaa actgaaccgc tcacgcgcct gtagctcacc
       61 ggatagagcg cttgcctgcg gagcaagagg cagcaagttc gattcttgcc aggcgcacca
      121 gtgtggccgc gagaaaactc acgccggcgc ggactcaaac ctcaacacac ccgcggccaa
      181 cgcctccgcg atcttctgct ggccggcagg atcgctcgcg atctgcgcct ctgacgggtt
      241 ggtgatgaac agcggctcca ccagcgcgcc cggcatctgg cttggattgt ctacccaccc
      301 cggtgactgc gggccgagct cgatgaggtg gttgtaactg tcgcctgatg ccgtgagggc
      361 cggggcggca agctgatcat cggtaaagat tccacggtcg gccacgccca accgcgcgac
      421 aagcgctgac tgcaggtcgg cagccagtcg gtggttggcg gcggcaaacg gcctggccgc
      481 gtcatagaac gtctccgtgc cgcctactga cgggtcgctg aagccgtcga aatggatcga
      541 aatgaggaca ttggcaccgg cggcattcgc gcacgccgcc cgcgcggcca ggtcgcgatg
      601 caccgcgctc gaggtgatcg ccccatagtt cgagtcggcc gccgccagct tgatcaccgt
      661 cgtatctcgc gtgcgcgaca tcaccacgct gtatccatcg gcgcgcaaga cagcggccag
      721 ccgagacgcc acggcaagcg tcgcgtcctt ctccagtacc tgatggccgg cggcggtcgc
      781 accgacgaca ccgggatcga gcccgccatg gccggcatcg atgaacaccg tctgaccgac
      841 gtgtccactc gtcggtgcaa ggctcacgca tgcgccagcc tcgaggccac ggagcagagg
      901 ctgcgagata gaaactgccg gcttgatctg aacgccggca acgtccgcct tcgccacgcc
      961 ctgtcccgtt gagtccatga cccggtagaa aacgagggcg acaacgacca caagcgccaa
     1021 tgctgacgct gtgcggagca gagccgaagg agcctgccgg cgccgagggc ggggtgggac
     1081 gttgcgcggt tttgcgatcg gcccgcgtag tctagcgggc cggggcgcac ccgaagccgc
     1141 ttaatggatc cggccgttct ccgccagcgc gatcttttta tccgcgccgg tgtcgatgtt
     1201 gaggatgccg aggatgacca tgaactgctt gttctgccag accgccacat agaacgcctg
     1261 gttggagatc gagatcgaca gcactatgga attcttgccc agaccggtgg cggttccttc
     1321 cgtcaccggg acctgcgacg ccttcagggt cggcgcgctg aagccgaaga tcgaatcggt
     1381 gctgtagccc ttctcggcgc tcgcctggtc cttgaactgg aacacgaagt tgaccaccag
     1441 cttgtacgtc gcggtgccta tctgcgagcc gttcgactcg agagcggtgc agtgcgccgc
     1501 ggagtccgtg tagaactcga catccgccgc cgtcgcgcca tccttctgcg cggccgccca
     1561 ctgacccttg atcgtcgaat acgtgatcgg atccttggtc tggatcttct tcaggtaatc
     1621 gttgatgtca cccgacagat tgcacctg
//
LOCUS       DASWGM010000546         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_176977,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000546 DASWGM010000000
VERSION     DASWGM010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176977"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VGA15_15675"
     CDS             complement(<1..1136)
                     /locus_tag="VGA15_15675"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX9589172.1"
                     /translation="MTDMRKETDSLGVVEVPADKLWGAQTQRSLEHFSIGRDLIPREM
                     ITAYATLKKAAATANHAGKRLDDQRYNLIVQTCDEILAGQHHDMFPLHVWMTGSGTQF
                     NMNVNEVISNRCCQLAGTPLGSKAPVHPNDHVNMAQSSNDSFPSAMYIAAAVNVKQRL
                     IPAVAALHDAISVKAQAWSDIVKIGRTHMQDATPLTLGQEWSGYAGMLADDLERIEAA
                     LQGVYRLALGGTAVGTGINSAPGFAEAAAAEIARLTGLPFLTAPNKFTVQGAHDALVQ
                     LSGTLRTLAVSLYKIGNDIRLMSCGPRAGFAELSIPENEPGSSIMPGKVNPTQCEALT
                     MIAAQVMANDVAVGFGGAGGYLEMNVYKPLIIFNITHSITIMTDG"
     gene            1367..>1860
                     /locus_tag="VGA15_15680"
     CDS             1367..>1860
                     /locus_tag="VGA15_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9589173.1"
                     /translation="MITADLSGKAALVTGGASGIGLATVELLLACGATVAVNHLPDDA
                     RARTELDRLRAGGGAVIAAPGDVSRPGEAEEMVRAAIDGLGRLDILVNNAGTPGTSAP
                     IAFANLDAMTEKFWQTILATNLLGPFRCSHAAAPALRRARGAIVNTASVAGLGRVGSS
                     IAYGA"
BASE COUNT          323 a          592 c          611 g          334 t
ORIGIN      
        1 ccatcggtca tgatcgtaat ggaatgggtg atgttgaaga tgatcagcgg cttgtagacg
       61 ttcatttcga gatagccgcc ggcgccgcca aagcccaccg cgacgtcatt ggccatcacc
      121 tgcgcggcga tcatcgtgag cgcttcgcac tgggtcggat tgaccttgcc cggcatgatc
      181 gaggagcccg gctcgttctc ggggatcgac aattccgcaa atccggcgcg aggtccgcag
      241 gacatcaggc gaatgtcgtt gccgatcttg tatagcgaca ccgccagggt gcgaagcgtg
      301 ccggacaact ggaccagtgc gtcgtgggcg ccctgcaccg tgaatttgtt gggcgcggtt
      361 agaaagggca ggccagtgag cctggcgatc tcggccgccg ccgcctcggc aaagccgggc
      421 gccgagttga tgcccgttcc caccgccgta ccacccaggg ccaagcggta caccccttgc
      481 agggctgctt cgatccgctc cagatcgtcc gccaacatcc cggcgtagcc ggaccattcc
      541 tggcccaatg tcagcggagt ggcgtcctgc atgtgggtcc gcccgatctt gacgatatcg
      601 ctccacgcct gagctttcac gctgatcgca tcatgcaagg ccgctaccgc gggaatcagg
      661 cgctgcttca cgttgacggc ggctgcgata tacatggccg acggaaaaga gtcgttcgac
      721 gactgcgcca tgttgacatg atcgttgggg tgaaccggcg ccttgcttcc aagcggcgtg
      781 cccgcgagtt ggcagcagcg gttggagatc acttcgttca cgttcatatt gaactgggtg
      841 ccgctgccgg tcatccacac atgcaacgga aacatgtcgt gatgctggcc ggcgaggatt
      901 tcgtcgcaag tctgaacgat caagttgtaa cgctgatcgt cgagccgctt gccggcgtgg
      961 ttcgcggtgg cagcggcttt cttcaaggta gcgtaggcgg tgatcatttc gcggggaatc
     1021 aaatccctgc cgatgctgaa atgttcgagc gagcgctggg tctgagcacc ccacagtttg
     1081 tctgcaggca cttcaaccac accgaggctg tcggtctctt ttcgcatgtc ggtcatgtga
     1141 tgtccctccg gatacgaggt tcgacgatgc tgttggcact atatcacaga cggaactccc
     1201 ccaaatcctc gcaggcgcgc gacggtggac cgtattgccg cgacggcctt cccccccgct
     1261 tgcacgggcg cgacggaaag agcacgattc gatcagaccg gacaccgatc tagactgcgc
     1321 gcctaagcgg ccgggcaggg ccccaccaga ggccagggag aaagccatga tcaccgcgga
     1381 tctcagcggc aaggcggcgc tggtcacggg cggcgcctcg ggcatcgggc tcgccacggt
     1441 cgagcttttg ctcgcctgcg gcgcaacggt ggcggtgaac catctgccgg acgacgcgcg
     1501 cgcccgcacc gagctcgatc ggttgcgcgc gggcggcggt gcggtgatcg ccgcgcccgg
     1561 cgacgtctcg cggccgggcg aggcggagga gatggtgcgc gcggcgatcg acgggctggg
     1621 acggctcgac atcctcgtca acaatgcggg gacgccggga acgagcgcgc cgatcgcctt
     1681 cgccaatctc gacgccatga ccgagaaatt ctggcagacc atcctcgcga ccaatctgct
     1741 cggcccgttc cgctgcagcc atgccgccgc gccggcactc cggcgcgcgc ggggcgccat
     1801 cgtcaacacc gcgtcggtcg cggggctggg gcgggtcggc agcagcatcg cctatggcgc
//
LOCUS       DASWGM010000547         3402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_734469,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000547 DASWGM010000000
VERSION     DASWGM010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734469"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGA15_15685"
     CDS             <1..444
                     /locus_tag="VGA15_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9589174.1"
                     /translation="GITLTGDAATKRHPTSIVGGQFSMFFTGAVALDQGRFGWDDYQR
                     LGDTAVDALADKFDVVQDDRLEIGRSHPFGARVSITTDDGVHERLYADPSGEPNSFPD
                     AQAMAQKFLTLARPVLNGRADQLADAILSLERFDRVEMATQLGRQ"
     gene            complement(452..1375)
                     /locus_tag="VGA15_15690"
     CDS             complement(452..1375)
                     /locus_tag="VGA15_15690"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9589175.1"
                     /translation="MTPPASRKARSTVAVIGLGSIGGVVAGCLRAADRHDVIACVRQP
                     IERLVLERPEGNVEVALRALTDPAQAGRVDWVLLCTKAQQTPLTAPWLVRFCDSSTRV
                     AVLQNGIGHVARVTPLIGDACVVPTLVYYNGERLGADRVRLRHVAGHDLAVADDANGR
                     AFAQLLEGTPLHVLLSDDFPTLAWRKLLINAVANPITALTLQRQAVFRRDDIKVLCVA
                     ILDEAVAVARADGAQLAEDESLRTMSTLLTYPPEAGTSMYFDRLAGRELEVEALTGAV
                     VAAGERLRIATPLNGAFLALLRAVSDAARTG"
     gene            1329..1733
                     /locus_tag="VGA15_15695"
     CDS             1329..1733
                     /locus_tag="VGA15_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589176.1"
                     /translation="MTATVERAFRDAGGVMTSFQAAKDMTSYLGHPVLDVLAEIARLP
                     RIPRGRGRGLPWKPGGNGSVGKLPWRNAVTALEMLCQRALIAKPDRDCCVGDRISEPE
                     TAAGRIQPQMGKINVRGGSDRALEQSDQLENG"
     gene            complement(1715..2332)
                     /locus_tag="VGA15_15700"
     CDS             complement(1715..2332)
                     /locus_tag="VGA15_15700"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX9589177.1"
                     /translation="MARPDTKNTTKYWWDRHISGLSLMHADFTCHDFTPHTHDAFVIA
                     VTELGGARIKSRGVTDVARSSGLFVSNPEEPQSSWMGDSPRWRYRSMYLTQAAIDVVA
                     EGLGIESVPYFTSNMIGDADLVSGFASLHQALEAGADRFREHEFLISAFGHLFGRRGS
                     GGDRIEPAPATASLFAGLLVWCGSDLPKIFFSAISPPLPGYPFSS"
     gene            complement(2437..>3402)
                     /locus_tag="VGA15_15705"
     CDS             complement(2437..>3402)
                     /locus_tag="VGA15_15705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX9589178.1"
                     /translation="PDRPPLRRTKALVVVKDGQVIAERYAAGYGIDTPMLGYSATKSV
                     TSALIGILVRQGKLAVNEPAPIAAWQSPDDPRHAITVDHLLRHTAGLAMGSSLNASLA
                     SAFDRVNRMKYVERDMAGFAENSELESAPGSAWNYHDGNTIILSRLIRDAAGGHAADV
                     LRFARTELFEPLGMRRVTLEFDATGTPEGSSQMLASARDWARFGLLYLNDGVIGGRRI
                     LPEGWVRYSASLTPGAWAGLGAGFWTNLGDSKGSRYRTSLGMPRDAYYASGVLGQYVI
                     IVPSERLVIARFGISGNQYDIAGVSRLVADVIAATGDKGRVARGD"
BASE COUNT          658 a         1082 c         1081 g          581 t
ORIGIN      
        1 ggcatcacgc tgaccggcga tgccgccacc aagcggcatc cgacctccat cgtcggcggc
       61 cagttctcga tgttctttac cggggcggtg gcgctcgatc agggccgttt cggctgggac
      121 gactatcaaa ggcttggcga caccgcggtc gacgcgctgg cggacaagtt cgatgtggtg
      181 caggacgacc gccttgaaat cggccgcagc catccgttcg gcgcccgggt ttcgatcacc
      241 accgacgatg gcgtgcatga gcggctctat gccgacccct ccggcgagcc gaattcgttc
      301 ccggacgcgc aggcgatggc gcagaaattc ctgacgctcg cccgcccggt gctgaacggc
      361 cgcgccgacc agctcgccga cgcgatcctg tcgctggagc gattcgatcg cgtggaaatg
      421 gcaacgcaac tgggacggca gtagggggcg gctatccagt gcgcgcggca tcgctgaccg
      481 cgcgcagcaa ggccaggaac gcgccattga gcggtgtcgc gattcgcagc cgctcgccgg
      541 cggcgacaac ggcccccgtc agggcctcga cttcgagttc ccgccccgcc agacgatcga
      601 agtacatcga ggtgcccgcc tcgggcggat aggtgagaag cgtcgacatt gttcgcagcg
      661 attcgtcctc ggccaattgc gcgccgtcgg cgcgcgccac cgctaccgct tcatcgagga
      721 ttgcaacaca cagcaccttt atgtcgtcgc gccgaaacac cgcctgtcgc tgcaacgtga
      781 gcgccgtgat tggattggca acggcattga tcagcaactt ccgccacgcc agcgtcggga
      841 aatcgtcact caggagaacg tgcaggggcg tgccctcgag cagttgcgca aacgcgcgac
      901 cgttcgcatc gtcggccacg gcgaggtcat gcccggcaac gtgacgcagg cgaacgcggt
      961 cggcgcctag ccgctcgccg ttataataaa ccagggtcgg gacaacgcag gcatctccaa
     1021 tcaaaggcgt gacgcgggcg acatggccga ttccattttg tagaacagcg acccgtgtcg
     1081 acgaatcgca aaatcgcacc agccatggag cggtcaacgg cgtctgctgg gctttggtgc
     1141 acaacagaac ccaatcgacg cgccctgcct gcgccgggtc cgtaagggcg cgcagagcaa
     1201 cctcaacgtt accttccggc cgctcaagca caagacgttc gattggctgc cggacgcaag
     1261 cgatgacgtc atgccgatcc gccgcgcgca ggcagcccgc cacgacgccc cctatgctcc
     1321 cgagaccaat gacggcgaca gtggaacgcg ctttccggga tgcgggtggt gtcatgacct
     1381 catttcaagc tgcaaaagac atgacctcct atctaggcca tcccgtcttg gacgtattgg
     1441 ctgaaattgc aaggctaccc aggattccgc gcggccgcgg gcggggcttg ccgtggaagc
     1501 cgggcggaaa cggcagcgtt ggcaaattgc cgtggcgtaa tgccgtaaca gcgcttgaaa
     1561 tgctttgtca gcgcgctctg atcgcaaaac ccgaccgcga ctgctgcgtc ggcgatagaa
     1621 tctccgagcc tgagacggcg gcaggccgca ttcagccgca gatgggtaag ataaacgtga
     1681 ggggtggctc cgatcgtgcg cttgaacagt ccgatcagct ggaaaatgga taacccggca
     1741 gcggcggcga gatcgccgag aagaagattt tcggcaaatc gctcccgcac caaaccagca
     1801 atcctgcgaa caaggatgct gtcgcggggg ccggctcgat gcgatcgccc ccgctgccgc
     1861 ggcggccgaa cagatgaccg aatgcggata tcaggaattc gtgctcgcgg aagcgatcgg
     1921 ctccggcttc gagtgcctgg tggagtgacg caaagccgct caccagatcg gcatcgccga
     1981 tcatattgct ggtgaaatac ggcaccgatt cgatcccaag cccctctgcg acgacgtcga
     2041 tcgccgcttg cgtcagatac atcgaccggt aacgccagcg gggactgtcc cccatccagg
     2101 aggattgcgg ttcttccgga ttggagacaa acagtcccga tgagcgcgcg acgtcggtaa
     2161 cgccccgact ttttattcgg gcaccgccaa gctccgtgac cgcgatgaca aatgcatcat
     2221 gggtatgcgg ggtaaaatcg tggcacgtga aatcggcatg catcaggctg agacctgaaa
     2281 tgtgcctgtc ccaccagtat tttgtagtgt tcttggtatc cggacgcgcc atttccgcgg
     2341 gtcttgcatg ctgagctttg ccaccgccta tctatgaggc cagaaccaat tagcaaaccg
     2401 cgaccgcgct tggacggcac gagcgcgatg ctcggctcaa tcgccgcggg cgacccgccc
     2461 cttgtcgccg gtcgcggcga tcacgtcggc aaccagacgt gaaacgccgg cgatgtcgta
     2521 ttgatttccg gagatgccga agcgcgcgat caccagtcgt tcggacggca cgatgatgac
     2581 gtattgcccg agcacgccgc tggcatagta ggcatcgcgc ggcatgccga gcgaggtacg
     2641 gtaccgcgaa cccttgctgt cgccgagatt ggtccagaat cccgcaccca agcccgccca
     2701 ggcgcctggg gtgagcgagg ccgaatagcg gacccatcct tcgggcagga tgcgcctgcc
     2761 gccgatgaca ccatcgttga gataaagcag gccaaagcgg gcccagtcgc gcgccgacgc
     2821 cagcatctgg ctcgatcctt ccggggtgcc ggtcgcgtcg aattccagcg tgacgcggcg
     2881 catgccgaga ggttcgaaca actcagttct cgcgaaacgc agcacgtcgg cggcatgacc
     2941 gcctgccgcg tcgcggatca ggcgcgacag aatgatggtg ttgccgtcgt ggtaattcca
     3001 ggcactgccc ggcgcgctct ccagttcaga gttttcggcg aatcccgcca tgtcgcgctc
     3061 gacatatttc atccggttga cgcggtcgaa ggcgctggca agcgaggcat tgagcgagct
     3121 gcccatcgcc agtccggcgg tgtggcgcag cagatgatcg accgtgatcg catggcgagg
     3181 atcgtccgga ctttgccacg ccgcgatcgg ggccggctcg ttgaccgcaa gcttgccctg
     3241 ccgaaccaga attccgatca gggctgaggt caccgacttg gtcgcggaat agccgagcat
     3301 cggcgtatcg atgccgtagc ctgccgcgta gcgctccgcg atgacctgac cgtccttgac
     3361 gacgacgagc gccttggtgc ggcggagcgg cgggcgatcg gg
//
LOCUS       DASWGM010000548         4079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1008354,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000548 DASWGM010000000
VERSION     DASWGM010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008354"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VGA15_15710"
     CDS             <1..465
                     /locus_tag="VGA15_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589179.1"
                     /translation="GHAASGMVRHGFVNTAATLSTVDRATAAFDVRKAKRDPEAASLS
                     GGNLQKFIVGREILRNPAVLVVSQPTWGVDAGAAAVIRQALLDLAAAGAAVLVTSQDL
                     DELAEIADRIAVMFHGRLSEPLATADANREKLGLLMGGSSLDQKEPAHAVGA"
     gene            449..1540
                     /locus_tag="VGA15_15715"
     CDS             449..1540
                     /locus_tag="VGA15_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589180.1"
                     /translation="MQLVLEKRAERSNTIALVSPLIAIGLTIVTMSILFAILGKNPIS
                     ALGVYFIDPLTDSYSLQEIAVKATPLVMIAIGLSLCYLANVWNIGAEGQFLVGAVAGS
                     WLAVKTQGTGAGHWVMPAMLLLGAVAGALYALIPAICKVRFGASEILTSLMLVYVADL
                     FLDYLVRGPWRDPAGFNFPTTAEFDPVATVPVLIEGGRLHLGAVITLVVVAAAAVLLG
                     RTIKGFEIRVVGAAPRAARFGGFNSNQLILLTFAISGALAGLAGIIEVAGPVGHLQPG
                     ISPGYGFTAIIVAFLGRLNPIGILIAGLFLALTFIGGEQAQIAMKIPLDVTKVFQGIL
                     LFYVLACDSLILYRFRLIFPTRKVPSGVA"
     gene            1527..2444
                     /locus_tag="VGA15_15720"
     CDS             1527..2444
                     /locus_tag="VGA15_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589181.1"
                     /translation="MGLLEAIILSVLAASTPLLIAATGELVTERSGVLNLGVEGMMIV
                     GAACGFGGAWLTGSTILGALCGIVAGTLLSLIFAMMTLGLAVNQVATGLALTILGIGL
                     SGLIGAGFVGERITPAPHLYIPGLTDIPLVGRILFGEDAFVYFSLALVIGVWLFLYRT
                     RTGLVLRAVGDNHISAHALGYPVLRIRMFAVMFGGACAGLAGAYLPLAYTPFFIPGMT
                     AGRGWIALALVVFASWRPGRLVVGAYLFGAVTILQLHAQGAGVGIPSQFMSALPYLAT
                     VIVLVLISRARTGGSTAPAALGTVFVPDR"
     gene            2518..3597
                     /locus_tag="VGA15_15725"
     CDS             2518..3597
                     /locus_tag="VGA15_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9589182.1"
                     /translation="MRKILIAATAVMLSAGASLFSASAADKLKVGFIYLGPIGDLGWT
                     YQHELARQAVVKEFGDKIETTFLENVPEGPDAERAIEQLVRAGNKLIFTTSFGYMEPT
                     LKVAKKYPNVHFEHATGYKRDANMSSYSARWYQGRYIQGIIAAKMSKSGVLGYIGSFP
                     IPEVVSGINSTILGAQTINPNIKVKIIWANSWFDPGKEADAAKALLDQGADVIMQHTD
                     SPAAMQVASERGKLAFGQDSEMIKFGPKAQLTSILDTWAPYYIARVKAELDGTWKSED
                     TWGGLETNMFAMAPYTNMPDDVKKMAEDTQAAITAGKLFPFKCPVVGQDGKEVECKGG
                     DHLADGQVLGMNFYVKGIDDKIPGK"
     gene            complement(3812..>4079)
                     /locus_tag="VGA15_15730"
     CDS             complement(3812..>4079)
                     /locus_tag="VGA15_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEX9589183.1"
                     /translation="GSAACVGRPELGEIAVGKAADLALFKLDELRFSGHGDPLAALVL
                     CGAHRADRVMVGGNWVVTGGAIPGLDMPDLIRRHSAAAHAMQVG"
BASE COUNT          704 a         1304 c         1258 g          813 t
ORIGIN      
        1 ggtcacgcgg ccagcggaat ggtccggcac ggtttcgtca acaccgccgc gaccttgtcg
       61 acggtcgacc gcgcgaccgc ggcgttcgat gtgcgcaagg ccaagcgcga tcccgaagcc
      121 gcaagcctgt ccggcggaaa cttgcaaaag ttcatcgtcg gccgcgaaat tctgcgcaac
      181 ccggccgtcc tggtggtcag ccagccgacc tggggcgtcg acgccggtgc tgccgccgtc
      241 atccggcagg ccctgctcga tctcgccgcg gctggcgccg ccgtgctggt gacaagccag
      301 gacctcgatg aactcgccga aatcgccgac cgaatcgccg tgatgttcca cggccgcctc
      361 tcggaaccgc ttgccaccgc ggacgccaac cgcgaaaagc tcggcctgct gatgggcgga
      421 agcagtctgg atcagaagga accggcccat gcagttggtg cttgaaaagc gagccgagcg
      481 atcaaacacg atcgcgctgg tttcgccgct gatcgcgatc ggtttgacga tcgtgaccat
      541 gagcattttg tttgcgatcc tcggcaagaa tccgatctcc gcgcttggcg tctatttcat
      601 cgatcccttg accgacagtt attcgctgca ggaaattgcg gtgaaggcga caccgctggt
      661 gatgatcgcg atcgggttgt cgctctgtta tctcgccaac gtctggaaca tcggagccga
      721 aggacagttt ttggtcggtg ccgtcgccgg cagctggctc gccgtgaaaa cgcagggcac
      781 cggcgccgga cattgggtga tgcccgcgat gttgctgctc ggcgcggtgg cgggcgcgct
      841 ctatgccctg ataccggcga tctgcaaggt ccggtttggc gccagcgaga ttcttaccag
      901 cctgatgctg gtgtatgtgg cggacctttt tctcgactat ctcgtgcgcg gtccctggcg
      961 cgatccggcc gggttcaatt tcccgaccac cgcggagttc gatccggtcg caaccgtgcc
     1021 ggttctgatt gaaggcggcc gtctccatct tggcgccgtc atcacgctgg ttgtcgtcgc
     1081 cgcggccgcg gttctgctcg ggcgcaccat caagggtttc gagatccgcg tcgtcggcgc
     1141 cgcaccccgc gcggcacgtt tcggcggttt caattccaac cagctgatcc ttctgacttt
     1201 cgctatctcc ggcgcgctgg ccgggctggc gggaatcatc gaggtcgcgg gaccggtcgg
     1261 gcatcttcag ccgggcattt caccgggtta tggtttcacc gcgatcatcg tcgcgtttct
     1321 cgggcgcctg aacccaattg gaatattaat tgcaggcctt ttcctcgcac tcaccttcat
     1381 tggcggcgag caggcccaga tcgcgatgaa aattccattg gacgtcacca aggtatttca
     1441 gggcattttg ctgttctacg tgctcgcctg cgacagcctc attctctatc gcttccggct
     1501 gattttcccg acgcgaaagg tgcccagtgg ggttgcttga ggccatcatt ctttcggtgc
     1561 tcgcggcgtc gacgccgcta ttgatcgcgg ccaccggcga actcgtcacc gagcgctccg
     1621 gcgtgctcaa tctcggcgtc gagggcatga tgatcgtggg cgcggcctgc gggttcggcg
     1681 gcgcctggct caccggctcg acgatccttg gcgccctctg cggcatcgtc gcgggcacat
     1741 tgctgtcgct gatcttcgcg atgatgacgc tgggcctcgc ggtcaaccag gttgccacgg
     1801 ggcttgcgct cacgatcctt ggcatcggac tatccggcct gatcggcgcc ggcttcgtcg
     1861 gcgagagaat cacgccggct ccgcatcttt acatcccggg cctgaccgac attccgctgg
     1921 tcgggcgcat cctgttcggc gaagacgcct tcgtttattt ctcgctggcg ctggtcattg
     1981 gcgtctggct gtttctctac cggacacgga ccggactggt gttgcgcgcc gtcggcgaca
     2041 atcacatctc ggcccatgcg ctcggatatc ccgtgcttcg gatcagaatg ttcgccgtga
     2101 tgttcggcgg cgcatgcgca gggctcgccg gagcctatct gccgctggcc tatacgccat
     2161 tcttcatccc gggcatgacc gccggccgcg gctggatcgc gcttgcgctc gtggtgttcg
     2221 cgtcgtggcg gccgggtcgt ctcgtggtcg gcgcgtatct gttcggcgcg gtgacgatcc
     2281 ttcaattgca cgcgcaaggc gcgggcgtcg gaatcccgtc gcagttcatg tcggcattgc
     2341 cgtatctcgc aaccgtcatt gttctcgttc tgatttcgcg cgccaggacc ggcggctcga
     2401 ccgcgcctgc tgccttggga acggtgttcg tgcccgatcg ataatacccg aagcgcgcgc
     2461 gacggggcgc gtgcgcatcg gcacagtcag tccacccctt agtgtcagga gaaacccatg
     2521 agaaagattc tcattgctgc gacggcagtg atgctcagcg caggagccag tctgttcagc
     2581 gcttccgctg ccgacaagct caaagtagga tttatctatc tcggcccgat cggagatctc
     2641 ggctggacct accagcatga acttgcgcgc caggccgtgg tcaaggaatt cggcgacaag
     2701 atcgagacca ccttcctcga gaatgtcccc gagggcccgg acgccgagcg cgccatcgag
     2761 cagctcgttc gcgccggcaa caagctgatc ttcacgacat cgttcggcta catggaaccg
     2821 accctgaagg tggccaagaa atatcccaac gtgcatttcg agcacgccac cggctacaag
     2881 cgcgacgcca acatgtcgag ctattcggca cgctggtatc agggtcgcta catccagggc
     2941 atcatcgccg ccaagatgtc gaagtccggc gtgctcggct atatcggttc attcccgatc
     3001 ccggaagtcg tctcgggcat aaactcaacg atactcggcg cacagacgat caacccgaac
     3061 atcaaggtca agatcatctg ggcgaactcg tggttcgatc ctggcaagga agctgacgct
     3121 gccaaggcgc tgctcgatca gggcgccgat gtgatcatgc agcataccga cagcccggcg
     3181 gcgatgcagg tcgcgagcga gcgcggcaag ctggcattcg gccaggattc cgaaatgatc
     3241 aagttcggac caaaggcgca gctcacctcg atcctcgaca cctgggcgcc ctactacatc
     3301 gcgcgcgtca aggccgagct cgatggcacc tggaaatccg aggatacctg gggcgggtta
     3361 gagaccaaca tgtttgcgat ggcgccctac accaacatgc ccgacgacgt gaagaaaatg
     3421 gctgaagata cccaggccgc catcaccgcg ggcaagctgt tcccgttcaa atgtcctgtc
     3481 gtgggccagg acggcaagga agtggagtgc aagggcggcg atcatctggc cgatggccag
     3541 gtgctcggca tgaacttcta cgtcaagggc atcgacgaca agattcccgg caagtaaggc
     3601 tgggcgctcc cgagactacc gggttcgggc ggcaacgccc ggaccctttt tatgttcggt
     3661 tgcagcggct acgttggtcc attgacgccc caaacgaaca tactccggcg tcatcctgag
     3721 gtgcgcgctc ttgcgcgcct cgaaggatgg acaacagggg gcggacgcca tccttcgagg
     3781 ctcgcttcgc tcgcacctca ggacgacgac ttcatcccac ctgcatcgca tgcgcggcgg
     3841 cgctgtggcg gcggatcagg tcgggcatgt cgaggccggg aattgcacca cctgtcacca
     3901 cccagtttcc tccgaccatc accctgtccg cgcgatgcgc gccgcacagg accagcgccg
     3961 ccaatgggtc gccgtggccg gagaaacgca attcgtcgag cttgaacagc gccagatcgg
     4021 cggctttccc gacggcgatc tcgccgagtt cgggacggcc gacacaggcc gccgatccc
//
LOCUS       DASWGM010000549         6196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2282667,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000549 DASWGM010000000
VERSION     DASWGM010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282667"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VGA15_15735"
     CDS             <1..880
                     /locus_tag="VGA15_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9589184.1"
                     /translation="PAIERRNGLLWYPSLYEGFEYSPNVIYTGASPNQNSFPLAEYLV
                     RNHGRRVFLVGSDYVYPRESNRIMRDLVESYGGEIVDEVYVPMDATEPCLRDLVARIT
                     DRAPNVVFSTVVGRAAQSFYRLYREAGLDPASLPIASLTMAEGEVQVIGAALCEGHIT
                     AATYFASLQGASNRRFADDFRRAFGPDRPISMWSAGAYAQVRLFAMALERAGTLDTQR
                     LVEAALGLSFEAPEGTIRIDPDNNHTWLTPRIGRLRRDGGFDVVWEAKAAVKPDPYLA
                     VSPLGARWIGDEVPVS"
     gene            877..1497
                     /locus_tag="VGA15_15740"
     CDS             877..1497
                     /locus_tag="VGA15_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX9589185.1"
                     /translation="MKPGIRRLIEDLRGARVLVVHPRDVEGDALIDQLKRIGCNVRGM
                     WPPPAELPNDVDTVFHLVETAETPAFVASASEDGPTFVAIVDYENPTVLKRLLDSNAH
                     GVVNKPIRPSGVLSTMVLARSMRGYTRRLEGKVAKLEETLKARRDVDKAVKILVSLKK
                     IGETEAYELIRSQATQKRLSMAQVATTVIGAQDVLTGLGLIGDGDR"
     gene            1743..2633
                     /locus_tag="VGA15_15745"
     CDS             1743..2633
                     /locus_tag="VGA15_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HEX9589186.1"
                     /translation="MTLHDPTHAATGTIVVACVQMQPAFGDVAANVAHSLDLIDQAAA
                     RGAKLVVLPELANTGYMFASRDEAFRLAEAIPGGTTVAAWAERAARHGLHLVAGITER
                     SGSDLYNSAVVIGPEGYIGTFRKVHLWNEENLFFEPGNLGFPVFHTAIGRIGVAICYD
                     GWFPETYRLCALQGADIVCVPTNWVPIPGQSEGREAMANILAMGAAHSNSIFIACADR
                     VGIERGQRFEGQSLIVSYTGWPVAGPASRDVEEILIAEVDLGEARRKRNWNAFNQVLR
                     DRRSDVYDEMLGSGLNRGWY"
     gene            2685..3854
                     /locus_tag="VGA15_15750"
     CDS             2685..3854
                     /locus_tag="VGA15_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HEX9589187.1"
                     /translation="MTFTRSLSALLAAAAASTLLAGSAFAADPIKIGVPVGLSGANSV
                     VAPSVVQSAELAVAEINAKGGVLGRPLALEVADDASGAAGAQKAFDSLVFQKKVDVLI
                     SMETSAARNAALPIVTRGKVPYIYTSFYEGKSCSPLMYVNAWVPEQQVPPIVDYFTKE
                     KAAKTFFLIGSDYAFGRGMLAFTKAYIEKTGGKVVGEEYLPMDGTDWTPIISKLKSAA
                     PGALITSTAGGAPNVTLTKQLRAAGVTLPYGNLAVDEGTAKGMGTDAEGIFLSASYVT
                     GIDSAANKTFLSAMQKKFGADLKTPNDLSVPEYEAVYAYKAAVEKAGKTEASAVLKAL
                     ADVAVDGPRGKIAMSQQHHAALTMYLGQVQADGGVKVIQSFPNVSAGQQCPNLKP"
     gene            3916..4752
                     /locus_tag="VGA15_15755"
     CDS             3916..4752
                     /locus_tag="VGA15_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9589188.1"
                     /translation="MITLALDIVTTAAILFIVAAGLLIVFGVMKIVNFAHASFITVGA
                     YAALMASRFGLPPVLAPFVAFLAGGLIGAATERIVVRRLYRRPLDAILATWGLGIVIG
                     QLVTLAFGREVQFVPAPISGTLAAFGVDYSLYRLLLVPTALFIAGLFAGLLNGTRLGL
                     STRAVIMNENLAQGLGIDSTRVRIITFGIGCGLAAVAGALITPLASVDPNMGLAWLIG
                     AFMLVMVSGGSLLTLGLSCFVLGGLQVLASTFISPILGGLTIAVLAAVALRLRPQGFA
                     RA"
     gene            4745..>6196
                     /locus_tag="VGA15_15760"
     CDS             4745..>6196
                     /locus_tag="VGA15_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9589189.1"
                     /translation="MPDLATVDDVAASAPLSGSSRPLLRVAIAAVVAAAVIVVGPWVL
                     DTYTVNILVRAFFVAIAALTVDVLWGVSGTLTFGQSAFFGIGGYALAIVFTQFGFGPG
                     EAMLAIGAAVGATVVVAGLVGWLSFYPGSTPLYASVISLVLPIVLVQILYSGGTFTGS
                     SSGLVGFESFDLTLEAWFRLSGLAVIATLVATTIALRSDAGRVLGAMRDNDERCSYLG
                     IDTARVRIGLLVVAAVIAALAGFGYVGFGAVAAPENAGFVFGTQLVVMVALGGRGSLI
                     GAVIGAVVIEVASAYLSSSLPFVWELIVGVAFVAVIVVLPGGLLGGLRDLGRAVRRRG
                     AAASAPVMLRTLEQPDAAPERNAIVVEVSGLSKHFGSLSVLSKISFTARQGELVSLVG
                     PNGAGKTTLIRCLADGRERSEGSVRIAGHSIERLPADRIVGLGLGRKFQAASVFETLS
                     VADCLRVARVRRERPSFLKTSPILTLPAAARAVV"
BASE COUNT          966 a         1929 c         2015 g         1286 t
ORIGIN      
        1 gcctgcgatc gagcgtcgca acggattgct ctggtatcca tcgctctacg aaggcttcga
       61 atattcccct aatgtgatct ataccggggc ctcgccgaac cagaacagct ttccgctcgc
      121 cgaatatctt gtccgcaacc atggacgccg ggtgtttctg gttggctccg actatgtcta
      181 tccgcgggaa tcgaatcgca tcatgcgcga tctcgtcgag tcctatggcg gcgagatcgt
      241 cgatgaggtt tatgtcccga tggatgcgac cgagccgtgt ctgcgtgatc tggttgcgcg
      301 gatcacggat cgggccccga acgtggtatt ctccacggtc gtcgggcgtg cggcgcaaag
      361 cttctatcgg ctctatcgcg aggccggtct cgatccggcg tcgttgccca tcgccagtct
      421 cacaatggcc gaaggcgagg tgcaggtgat cggtgcggcg ctgtgcgaag ggcacatcac
      481 cgcggcgacc tatttcgcgt cgctgcaggg cgcaagcaac cgacgtttcg cggacgactt
      541 ccgtcgcgcc tttggccccg accggccgat cagcatgtgg agcgccgggg cctatgctca
      601 ggtccgcctg ttcgccatgg ctttggagcg cgctggaaca ctcgacacgc agcggctggt
      661 cgaggctgca cttggcctgt cattcgaagc accggaaggt acaattcgga tcgatccaga
      721 caataaccat acctggctga cgccgcgtat cggacggctc cgtcgcgacg gcgggttcga
      781 cgtggtctgg gaggcaaaag ccgcggtgaa gccggatccc tatctggcgg tgtcgccgtt
      841 gggcgcgcgc tggatcggtg acgaggtgcc ggtctcatga agccgggcat ccgccgcctc
      901 atcgaagatc tgcgtggtgc tcgcgtcctt gtcgttcatc cgcgcgacgt ggaaggtgac
      961 gcgctgatcg atcaattgaa gcgcatcggc tgcaacgtgc gcggcatgtg gccaccgccg
     1021 gcggaattgc ccaacgacgt ggacacggtg tttcatctgg tggagaccgc cgaaacaccg
     1081 gctttcgtcg cctcggcttc cgaagacggg ccaaccttcg tcgccatcgt cgactatgag
     1141 aatcccacgg ttctgaagcg gctgctcgat agcaatgccc atggcgtcgt caacaagccg
     1201 atccgtccct ccggcgtcct gtcgacgatg gtgctcgccc gctcaatgcg cggctacacc
     1261 cgccggctgg agggaaaggt ggcgaagctc gaagaaaccc tgaaagctcg ccgtgacgtc
     1321 gacaaggcgg tgaagatatt ggtgtcgctc aaaaagatcg gcgagaccga ggcctatgag
     1381 ctgatccgat cgcaggcgac gcagaagcgg ttgtcgatgg ctcaggtggc cacgacggtt
     1441 atcggcgctc aggacgtgct gacagggcta ggcctgatcg gtgacggcga tcgttgagcc
     1501 agtcttgatc ggatgtttgc acaatgggta gtcaacggtg ctgataattc tatcctgcag
     1561 caagattgac attgagacac ggttgctgct gtaattcgaa tgttggtaat gttcgaccag
     1621 cccggtcggg atcaccacag cggccggtcg gccgtttcaa catctggcta tgtagccctc
     1681 gatcggtgcc tgacggcgct ggcgggggtt ttttgtttgc gcgatccagg gagctctgcg
     1741 cgatgacgct tcacgaccca acgcatgcgg cgacaggaac gatcgttgtc gcctgcgttc
     1801 agatgcagcc ggcgttcggg gatgttgccg cgaacgtggc acatagtctc gacctgatcg
     1861 atcaggctgc cgcgcgcggt gccaagctcg tcgtgctgcc ggagctcgcc aataccggct
     1921 acatgttcgc gtcgcgcgac gaagccttcc gccttgccga agccatcccg ggcgggacaa
     1981 cggttgcggc ctgggcggag cgcgcggcgc ggcatggcct gcacctcgtc gcgggcatca
     2041 ccgaacgctc gggctcggac ctctacaaca gcgcggtcgt gatcggtccc gaaggctata
     2101 tcggcacgtt ccggaaggtg catctgtgga atgaggaaaa cctgttcttc gagccgggca
     2161 atctcggctt cccggtgttc cacaccgcga tcggccggat cggcgttgcg atctgctacg
     2221 atggctggtt tcccgagacc taccggctct gcgcgctgca gggcgcagat atcgtctgcg
     2281 tgccgaccaa ttgggttccg attcctggtc agtccgaagg gcgcgaggcg atggcgaaca
     2341 tccttgcgat gggcgccgcg cacagcaatt cgatcttcat cgcctgcgca gatcgtgtcg
     2401 gcatcgagcg cgggcagcgc ttcgaggggc agagcctgat cgtcagttac accggttggc
     2461 ctgtcgccgg gccggccagt cgtgacgttg aagaaatcct catcgccgag gtcgacctcg
     2521 gcgaggcccg gcgcaaacgc aattggaatg ccttcaacca ggttctgcgc gaccgccgct
     2581 cagacgttta tgacgagatg ctcggttccg gcttgaatcg cggctggtac tgagcatcgc
     2641 ctcgcatcct taataattca ctcaaccctc aggagtaccg atccatgacc ttcacccgtt
     2701 cgctctcggc actgctcgcc gccgccgcag cctcgaccct cctcgccggt tcggcattcg
     2761 ccgccgatcc gatcaagatc ggcgtgccgg tcggcctctc cggcgccaac agcgttgtcg
     2821 cgccctcggt ggtgcagtcg gccgaactcg ccgtcgccga gatcaatgcc aagggcggcg
     2881 tgctcggccg cccgttggcg ctggaagtcg ccgatgatgc gtcgggcgct gcaggcgcgc
     2941 aaaaggcttt cgacagcctg gtgtttcaga aaaaggttga cgtgctgatc tcgatggaga
     3001 ccagcgcggc gcgcaatgcg gcgctgccga tcgtgacgcg cggcaaggtg ccttacatct
     3061 atacgtcgtt ttatgagggc aagtcctgca gtccgctcat gtatgtcaat gcatgggtcc
     3121 ccgaacagca ggtgccgccg atcgtcgatt atttcaccaa ggaaaaggcg gccaaaacgt
     3181 tctttctgat cgggtcggat tatgccttcg gccgcggcat gctggccttc accaaagcct
     3241 atattgaaaa aaccggcggc aaggtggtcg gcgaagaata tctgccgatg gacggcaccg
     3301 attggacgcc gatcatctcc aagctgaaga gcgcagctcc gggcgcgctg atcacctcga
     3361 ccgccggggg cgctccgaac gtaaccctga ccaagcagct tcgcgccgcc ggcgtcacct
     3421 tgccctacgg caatctcgcg gttgacgagg gtacggcgaa aggcatgggg accgatgcgg
     3481 aaggcatctt cctttccgcc tcttatgtta ccgggatcga cagcgccgca aacaagacct
     3541 tcctgagcgc gatgcagaag aagttcgggg ccgatttgaa gaccccgaat gatctttcgg
     3601 tgccggaata tgaagcggtt tatgcgtaca aggcggccgt ggaaaaggcc ggcaaaaccg
     3661 aagcgagcgc cgtgctgaaa gcccttgccg atgtcgcggt ggatggtccg cgcggcaaga
     3721 ttgcgatgtc gcagcagcat cacgcggcgt tgacgatgta tctcggtcag gtgcaggctg
     3781 atggcggcgt caaggtcatc cagagctttc ctaatgtcag cgccggacag cagtgtccga
     3841 acctgaagcc ctgagctgcc tgcgccaagc gggaggacgc atcgtcctcc cgccgcggtc
     3901 gcccggtgct gtctcatgat cacgctcgcc ctcgacattg tcactacggc cgcgatcctt
     3961 ttcatcgtcg cggccgggct gctgatcgtg tttggcgtga tgaagatcgt caatttcgcc
     4021 cacgcctcct tcatcacggt cggcgcctac gccgcgctca tggcctctcg cttcggcctg
     4081 ccgcctgtgt tggccccgtt tgttgcgttc ctcgctggcg gactgatagg ggccgcgacc
     4141 gaacgcattg tggtgcggcg gctctatcgt cgaccgctgg acgcgatcct ggcgacctgg
     4201 ggcctcggca tcgtcatcgg ccaattggtc acgctggcat tcggtcgcga agtgcagttc
     4261 gtgccggcgc cgatctcggg gacgctggcg gcgttcggcg tggactattc gctgtaccgg
     4321 ttgctgctgg ttccgaccgc gctcttcatt gccgggttgt tcgccggcct tctcaatggc
     4381 acgcgacttg gtctgtcgac ccgcgcagtc atcatgaacg agaatttggc gcagggactt
     4441 ggcattgaca gcacccgcgt ccgcatcatc acgttcggga tcggctgcgg gctggccgcg
     4501 gtcgccggcg cgctgatcac cccactggcg agcgtcgatc ccaacatggg tctggcctgg
     4561 ctgatcggcg ccttcatgct cgtgatggtg tccggcggct cgctgctgac gctcggtctg
     4621 tcctgcttcg tcctcggtgg gcttcaggtt ctggccagca ccttcatcag tccgatcctt
     4681 ggcggattga ccatcgcggt gctcgccgcg gtggcgcttc ggcttcgtcc gcaggggttt
     4741 gcccgtgcct gatctcgcca ccgttgatga cgtcgcggcg tcggcgccat tgtccggctc
     4801 gtcgcgtccg ttgctgcggg ttgcgatcgc ggccgtggtc gcggctgcgg tgattgtcgt
     4861 cggcccctgg gtactggaca cctacacggt caacattctg gtccgcgcgt ttttcgtcgc
     4921 gatcgcagcg ctgacggtgg atgtgttgtg gggggttagc ggcacgctga cattcgggca
     4981 atccgctttc ttcgggatcg gcggttacgc cctggcgatc gtcttcaccc aattcggctt
     5041 tggcccgggt gaagcgatgc tggcgatcgg ggccgcggtc ggggccaccg ttgtggtggc
     5101 gggtttggtc ggctggctgt cgttctatcc cggttcgacc ccgctctatg cgtccgtgat
     5161 ctcgctggtg cttccgatcg tcctggtcca gatcctgtat tccggcggca cctttaccgg
     5221 atcctccagc ggcctggtcg gcttcgagag tttcgatctc accttggagg cctggttccg
     5281 attgtccggt ttggccgtga tcgccaccct cgtcgcgacc acgatcgcgc ttcgcagtga
     5341 cgccgggcga gtgctcggcg cgatgcgcga caatgacgag cgctgcagct atctggggat
     5401 cgacacggcc cgggtccgca tcggtctcct ggtcgtcgcg gccgtgatcg cggcactggc
     5461 cggattcggc tacgtcggat tcggcgccgt cgccgccccc gagaatgccg gcttcgtgtt
     5521 cggcacccag ctggtcgtga tggtggcgct tggcggccgc ggatccctga tcggcgccgt
     5581 catcggcgcc gtggtgatcg aggtcgccag cgcttatctc tcctctagtc tgccctttgt
     5641 ctgggagctg atcgtcggcg tcgccttcgt tgcagtgatc gtcgtgcttc cgggcgggct
     5701 gctcggcggc ctgcgcgatc tcggccgcgc ggtgcgccgg cggggcgcgg cagcgtcggc
     5761 accggtcatg ctccgcactt tggagcagcc cgacgccgca cccgagcgca acgcgatcgt
     5821 ggtcgaggtt tccgggctct ccaagcattt cggcagcctg tcggtgctgt ccaaaatctc
     5881 gtttacagcg cgacagggcg aactcgtcag tctggtcgga ccgaacggcg caggcaagac
     5941 caccttgatc cgctgtctcg cagatggccg cgagcgctcg gaaggcagcg tgcgcattgc
     6001 cgggcactcg attgagcgtc tgccggccga ccgcattgtc gggctcgggc ttgggcgcaa
     6061 attccaggct gcctcggtgt tcgaaacctt gagtgtggcc gattgtctgc gggtcgcccg
     6121 ggtcaggcgt gagcggccgt ccttccttaa aacctcgccg atcctgacct taccggccgc
     6181 cgcgcgcgcg gtggtg
//
LOCUS       DASWGM010000550         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_66678,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000550 DASWGM010000000
VERSION     DASWGM010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66678"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="VGA15_15765"
     CDS             complement(<1..1808)
                     /locus_tag="VGA15_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HEX9589190.1"
                     /translation="MSARKVDDDRRRQHSSASVARHLAHRSVIGLWSRLSRFDRAVLV
                     VFVLVLIIAAIGPFVAPYPPTFADPLQRLVSPNGAHPFGTDENGIDVLSRLIAAPRTD
                     VLIAVIATTLSVAIGAPLGVVAGYFEGTDRRAMRWISEGTLRLLDVIQAFPVFILAMV
                     LVAIRGTGPVNVVFAVAFVNFPVFLRLVRSEILSLREHLYAEAAVALGNSELGVGFKH
                     LLPNAWPTVLVQVSVTIGFAVLLTAGLSFVGAGVSPPTPELGAMIASGAKFMITGQWW
                     VAMFPGLLLGLIVFTFAVMGDIVAKLMEPGQRAIPSRRLLSKDRIAPAARAEAAAPSA
                     QDAVLSVRGLAIEASGRPPILDGVSFDLMPGEILGVVGLPGSGKSILIRAIVRLLGHG
                     LQRRAGHVFFRGKDLAELDEKTLRAIRGYEILPLLPNAKSQLNPLVRVGDLMVAHIRA
                     HYQCSRREAHARAARMLGAIGIGDAEHWLAAYPHELSGGMAQRVCLAIALVHRPPLII
                     ADEPTAGLDVTVQRQVLDLMIELCHQSGAAQVLATRELGIVAHYCNRVAVLHEGLIVE
                     MGSVRDVLLAPRHTVTRRLIAAADLRAVSAAATTNTS"
     gene            complement(1805..>2042)
                     /locus_tag="VGA15_15770"
     CDS             complement(1805..>2042)
                     /locus_tag="VGA15_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9589191.1"
                     /translation="AVVVTGVVAGYLLGGAVLIETVFNLNGIGQYAVQSITTADYAPI
                     QGFVLVAAVFTMMVYLIVDLLQFMIDPRVSARAS"
BASE COUNT          472 a          624 c          618 g          328 t
ORIGIN      
        1 gatgtgttgg ttgtcgcggc agcgctgacg gcgcgcaagt cggcagctgc aatcagtcta
       61 cgagtaacgg tgtggcgagg ggccaacagc acatctcgaa cggaccccat ttcgactatc
      121 agaccttcgt gcagaaccgc aacgcggttg caataatggg cgacgattcc gagttcgcga
      181 gtcgccagga cctgggcggc gccactttgg tgacaaagct ctatcatcag gtcgagtacc
      241 tgtcgttgca cggtcacatc aaggccagcc gtcggctcgt cggcgatgat tagcggcggc
      301 cgatgtacca gtgcgatagc aaggcagacg cgctgcgcca tgccgccgct caactcgtgc
      361 ggataggccg cgagccagtg ctccgcgtcg ccgatcccga tcgcgcccag catcctcgcg
      421 gcgcgcgcat gtgcctcacg gcgggaacac tgatagtggg cccgaatatg cgccaccatc
      481 agatcgccaa ctcgcaccag cggattgagc tggctcttgg cgttggggag cagaggcagg
      541 atctcgtagc cgcgaatggc gcgcagtgtc ttttcatcga gttcggccag atccttgccg
      601 cggaaaaaga catggcccgc ccgccgctgc agaccgtggc cgagcagcct aacgatggcg
      661 cgaatgagga tggacttacc ggaaccaggg aggccgacta cgccgagaat ctcgccgggc
      721 atgagatcaa agctcacgcc gtcgaggatg ggagggcgac cagaggcctc gatagcgagc
      781 ccacggaccg agagtaccgc gtcctgtgcg gaaggtgcgg cggcttcggc ccgcgccgca
      841 ggcgcgatgc ggtctttcga taagagacga cgcgagggaa tggctctttg gcccggctcc
      901 atcagcttgg ctacaatgtc gcccatgacg gcaaatgtga agacgattag gcccaacaag
      961 agccctggaa acatcgccac ccaccactgt ccggtgatca tgaacttggc gccgctcgcg
     1021 atcatcgctc cgagttcagg cgtaggcggg ctaacgccgg cacctacgaa actcagtccg
     1081 gcggtcaata gcacggcaaa gcctatcgtt accgacacct gaactaggac tgtcggccag
     1141 gcgttcggaa gcagatgctt aaaaccgacg ccgagttcgg agttgccgag agccaccgca
     1201 gcctcggcat aaagatgctc gcgcagggaa agtatttcag atcgcaccag acggagaaac
     1261 accggaaaat tcacgaaagc gactgcgaaa accacgttaa ccggtccggt gccgcgaatc
     1321 gccaccagca ccatggccaa aatgaagacc gggaacgcct gaatgacgtc gagaagacgc
     1381 agtgtcccct ccgatatcca tcgcatcgct cgccgatctg ttccctcgaa ataaccagcg
     1441 acaacgccaa gcggcgcgcc gatcgcaacc gagagggtgg tggcaatgac ggcaatgagt
     1501 acgtcagtgc gaggagctgc gatgagccgg gacaggacat cgatgccgtt ctcgtctgtc
     1561 ccgaaaggat gcgcgccatt cggagacacc agccgctgca gcggatcagc aaatgtcggc
     1621 gggtagggcg caacaaacgg gccgatggct gctataatca gaacaagaac aaagacgacc
     1681 aaaaccgccc ggtcgaagcg gctgaggcgc gaccacagtc cgatgacgct ccggtgcgct
     1741 aaatgccttg ctacgcttgc agacgaatgc tgccggcgcc tgtcgtcgtc aaccttgcgc
     1801 gcgctcaaga tgccctcgcc gagacacgcg gatcgatcat gaactggagg aggtcgacaa
     1861 tcagatacac catcatcgtg aagaccgcag cgaccagcac aaagccctgt atcggcgcat
     1921 agtctgccgt cgtgatcgac tgcacagcat actggccgat cccgtttaga ttgaaaactg
     1981 tttcgatcag aactgcgccc ccgagcagat atccggcaac aacgcctgtg accacaactg
     2041 cc
//
LOCUS       DASWGM010000551         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1853973,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000551 DASWGM010000000
VERSION     DASWGM010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853973"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..1919)
                     /locus_tag="VGA15_15775"
     CDS             complement(321..1919)
                     /locus_tag="VGA15_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589192.1"
                     /translation="MKLLKGILVGGVLISTGQIFAQQVFAQQAVAEADTSSEIRLLKA
                     KLKQLEQRVEDQGRKEKQIEAQANAATPPAYKASPSAFDPCPAGKVCYKGVTLTFGGW
                     VDLTGIYRTRNLASDTGSVYNFIPYPQAKNFNTPESRFSSRQTRFSVLAEGDALDTHI
                     AGYGEIDFEGAAQTANSVATNSFNPRMRQLSVEFDRKDLGFHVLAGQSWSLNAPSKAG
                     IDPRGVDAPGVIDFESVPGFLAARQPGIRVWQDIGPEFKIAASVENPQTAFFGGNTPT
                     VGTPAVGPQGVLNPNLLVNLTGPGGSFFNNANNLSLNQVPDVTVKAAWDPRLGPYKLH
                     IEAWGLFRQFFDRFNFANHTYDTGSFGGHISAEVIPQTLELQVYGAHGALGRFTAAPF
                     PDATLAQDGTILPLSVTSALVGVVWHAMPTLDIYGYAGLEKVDAAFRNVGTVPFGYGN
                     PLYNNTGCTVENSPAATCNGNTSEVRQYTVGFYDTVMKGNYGTVKAGVQYSYNQRFAF
                     AGVGGAPKTDDNIVMTQIRYYPFQ"
     gene            complement(2550..>3339)
                     /locus_tag="VGA15_15780"
     CDS             complement(2550..>3339)
                     /locus_tag="VGA15_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEX9589193.1"
                     /translation="IVNWSKARSSVVGLPAIPQRASIVFDDPSIVRLDGSAQAPLAGA
                     KHIELHGRLVEGAALDRPAIETVLRIAGGSVQGLHPLLAASFDADVRTMLSGLKDFSP
                     RPWPERFREIQAAGGHVEIVQSRIQQGDLIAVAAGTLGLTTGGRLDGELQMTVAGIEK
                     VIPALGIEKMLDEGVPQTTLDRVAPGVKTQDVNNLLGALDRAIPGLGKVVKQNANVGV
                     AAGINALGKEAVLEGKKARAFPLRFVDGAVFLGPLKVAQIPPLF"
BASE COUNT          629 a         1044 c         1014 g          652 t
ORIGIN      
        1 agatcgtcca atgccgccac ctgcatgctg atctgactgc gttccggcac ccgcagtcgc
       61 agcgcaccgc gcccctcggc ctgcggtctt tcctgctcgg gtaaaccttc gaacaattgc
      121 tcgtcagcca tcgtcagccc ctgcttcgcg cgcaatgcga gcgaatcaca gcactgccgc
      181 tgtggcaaat gctcattgcc aaaagattct cacgctctca ggatgacgtc gggggtgtgg
      241 cgcaccaaga aatcgtcgta ggcgcacgat caaacctgca ccgatttgca tgaggacgcc
      301 gaacagccac ccacgcgagg tcattggaac gggtaatagc gaatctgggt catgacgatg
      361 ttgtcgtccg tcttcggcgc gccgccgacg cctgcgaacg caaagcgctg gttgtaggaa
      421 tactgcacgc cggctttgac cgtgccgtag ttgcccttca tgaccgtatc gtagaatccg
      481 acggtgtatt gccgcacttc cgaggtgttg ccgttgcagg tcgccgccgg cgagttctcg
      541 acggtgcagc cggtgttgtt atagagcggg ttgccgtagc cgaacggcac cgtgccgacg
      601 ttcctgaagg ccgcgtcgac tttttccaac ccggcgtagc cgtagatatc gagcgtcggc
      661 atcgcgtgcc aaaccacgcc caccagcgcc gaggtgacgg acaagggaag gatcgtgcca
      721 tcctgcgcca gcgtcgcgtc cgggaacggt gcggcggtga agcgcccaag cgcgccgtgg
      781 gcgccgtaga cctgcagctc caacgtctgc gggatcacct ccgcgctgat atggccgccg
      841 aagctgccgg tgtcgtaggt atggttggcg aagttgaagc gatcgaagaa ctggcgaaag
      901 agcccccaag cctcgatgtg cagcttgtaa ggcccgagcc gagggtccca cgcggccttc
      961 accgtcacgt cgggcacctg gttcaagctg agattgttgg cgttgttgaa gaagctgccg
     1021 ccgggaccgg tcaggttgac aagcaggttc gggttcaaca ccccctgcgg cccgaccgcc
     1081 ggcgtgccga ccgtcggcgt gttgccgccg aagaaggcgg tctgcgggtt ttcgaccgat
     1141 gccgcgatct tgaactccgg accgatatcc tgccagaccc ggattccggg ctgccgggcc
     1201 gcgagaaatc ccgggaccga ttcgaaatcg atcacgccgg gcgcatcgac gccgcgcgga
     1261 tcgatgcccg ctttgctcgg ggcattgagc gaccatgact ggccggcgag gacatgaaat
     1321 ccgagatcct tgcgatcgaa ctcgacgctg agctggcgca tccgcggatt gaacgaattg
     1381 gttgcaaccg aattcgcggt ctgtgccgcg ccttcgaaat cgatttcgcc gtagcccgca
     1441 atgtgcgtat ccaaggcgtc gccttcggcc agcaccgaga acctggtttg gcgcgacgaa
     1501 aaacgcgatt ccggcgtgtt gaaattctta gcctgcggat agggaatgaa gttgtagacc
     1561 gatccggtat cggaggcgag gttgcgggtc cggtagatgc cggtgagatc gacccaacca
     1621 ccgaaggtca acgtaacgcc cttgtagcag acctttccgg caggacatgg atcgaatgcc
     1681 gacggcgatg ccttgtaggc gggcggcgtg gcggcattgg cctgtgcttc aatttgcttt
     1741 tccttgcggc cctggtcctc gacgcgctgt tcaagctgct tcagcttcgc cttgagcaaa
     1801 cgaatctcgg acgacgtatc cgcttccgcg acggcctgtt gcgcgaagac ctgttgcgcg
     1861 aagatttggc ctgtcgaaat cagaacgccg ccaacaagaa tccccttgag taacttcact
     1921 agaacctccc gcccaggaag cgacacgcgc acatcgcacc tcaataaggg tgtgtgcatg
     1981 cttcctctga gggagagacc ggagttcaag cgccgatatt cctcgatcgc cggattattt
     2041 ttgcatcatg ttttactcac gacgaagtgt tggaattttt ttgaaagctg ccgaacgcgc
     2101 gcgctcggcg cgaattgcga acccaagatg ggcacgactc agcgcagcca gtggagccgg
     2161 aagttcaacg cagcaacgtc gcagccttaa gttattctcg tcgtgatgct tgctgaaatt
     2221 ttaatctgat cattggtctc ctcctgcaat cgacccggtg cattcgtgcg ggtcgtcgga
     2281 gggtaagact gatgttcgcg cattggcatt cccggcgcgt gccgaaatgt cgatcacggt
     2341 tcggcgataa ggggctttca cgaagcggcg caggcggcca cgcaaaatct atcgtcgtcc
     2401 ccgcgaaggc ggggacccat aatccccggc gtcgctgttg acacaaattg cgcggagtaa
     2461 ctttcttcgc ttcagaagcg ccggtggtta tggatcccgg ctcgcgctcg caagcgctcg
     2521 cttggccggg acgacggcgg tgtgcttagc taaaacagcg gcgggatctg cgccactttc
     2581 agcggcccca aaaataccgc gccgtcgaca aaccgcagcg gaaacgcccg tgcctttttg
     2641 ccttccagca ccgcttcctt gccaagcgcg ttgatgccgg cggcgacgcc gacattggcg
     2701 ttttgcttca ccaccttgcc gagccccgga atcgcccggt ccagcgcgcc gagcaaattg
     2761 ttgacgtcct gggttttgac gccgggcgcg acccgatcga gtgtcgtctg cggcacgcct
     2821 tcatcgagca ttttttcaat cccaagcgcc ggaatgacct tttcgatacc ggcgaccgtc
     2881 atctgcaatt cgccatcgag acggccgccc gtggtcaggc ccagggtgcc ggcggcgacc
     2941 gcgatcagat cgccttgttg aatgcgggac tgcacgatct cgacatgccc gccggcagcc
     3001 tggatctcgc gaaaccgctc gggccagggt cttggcgaaa aatccttcag accgctcagc
     3061 atcgtccgca cgtcggcatc gaacgacgcc gcgagcagcg gatgcagccc ctgcacgctg
     3121 ccgcccgcga tccgcagcac ggtttcgatc gccggacgat cgagcgccgc accttcgaca
     3181 agccggccgt gcagctcgat gtgttttgcg cccgcaagcg gcgcctgcgc cgatccatcg
     3241 aggcgaacga tcgagggatc gtcgaacacg atcgaggcgc gctgcggaat cgccggcagt
     3301 cccaccacgc tgctgcgcgc cttgctccag ttcacgatc
//
LOCUS       DASWGM010000552        13136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1286272,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000552 DASWGM010000000
VERSION     DASWGM010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13136
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286272"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(762..1823)
                     /locus_tag="VGA15_15785"
     CDS             complement(762..1823)
                     /locus_tag="VGA15_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9589194.1"
                     /translation="MHSKTDTVQSSAEALRYPWENHPGPDQVVEVMPGVLWVRLALPF
                     RLNHVNIYLLADGDGWAMVDSGFGNEESIAAWTALFEGPLAHVKITRLIVTHSHPDHV
                     GLAGWIVERFNCPLYMSQVEYLQSVYHQNRGTEERKMAQRLFFRRHGMDESLTDKLLG
                     RGQDYLKRVSILPPSYRRISHGDEISIGARRFKVITGGGHALDQVMLYCAADKLFLSS
                     DQVLSKISPNVSVWAVEPDQNSLGEYLASLASLTTTLPYDLLVLPGHGVPFYGLKTRI
                     KQLADHHEDRCRLIADACREISKTSKELVPVVFHKHVLDVHQMGFAAGELIAHVNYML
                     VEGRLTAKENDGVLHFRTT"
     gene            complement(2046..4193)
                     /locus_tag="VGA15_15790"
     CDS             complement(2046..4193)
                     /locus_tag="VGA15_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9589195.1"
                     /translation="MALRSTMGKLRGLGFRRPKWGVRGSLFAAFAVIAGMAIVISAGA
                     GMVLGHLGGSMLDLSGRDIPRLAASLQLAAQSASLASQGPALLASGSEEALNERARKM
                     KETQHDAVDKLGQITELGADKTVVAALGETVKSIDDTIKSLGAAATERLRIAAQREKQ
                     YDALRKAQTAFAAAAGPAMIDAQTRIRAILGSANLLSQDDAIEAAQAIEQLGNVIAST
                     NLLALHMMAALSATNGDALEQVEKAFKAAYPNIKSNLDSLTKNNDTAALKDAALKLLA
                     FGDGKTSVFKTRQKELDADDYGQLILDETRKLNVGLGISVKQLVDGVQTETDASTWQA
                     RREISFATVVMLALGGLTFIGSVLFVWLYDGRNILRRIGNLQRSMQLLSGGDLDTEIH
                     RSRQRDEIAAMANSLEIFRGSMIEARALSTEQDKDRIVKAERASRMETRIVEFEATVR
                     AALDGLQTSADSMQTTAQRMSATADQSSALVSAVASAAEETSANVQTVSSGTEELSSS
                     ITEIGRQVITSAEIARKAVDEAGQTDATMQGLAENASRISVVVDLIQVIASQTNLLAL
                     NATIEAARAGDAGRGFAVVASEVKSLANQTAKATDEIRAQIASMQQVTTTAVNAIRNI
                     GNTIGEINEVTTAIAAAVEEQGTATREIARNIQHAAGGTSEVSSNIVGVSTASAEAGS
                     AAGEVLSASGALRRETDVLRSEIDAFLSNIRAA"
     gene            complement(4366..5076)
                     /locus_tag="VGA15_15795"
     CDS             complement(4366..5076)
                     /locus_tag="VGA15_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9589196.1"
                     /translation="MKKFLLGTVALVALGAAAPAMAADLAARPYTKAPAYAAPIYNWT
                     GFYIGGHVGGAFAGNNSLIGNDGRFLGGVQGGFDYQFAQSWVVGVEAQYSWLSNNNNG
                     VVFPGGVLVTSKNDQLGSVTGRLGYTWGPALLYAKGGYAWKDGNNLNVTTGGVPVAFT
                     TNGNHRDGYTVGAGLEYMFAPSWSAKVEYQYYNFGSTTFTTGPAVIVGTRFRDDEHTV
                     KAGINYRFNWGGPVVAKY"
     gene            complement(5457..5777)
                     /locus_tag="VGA15_15800"
     CDS             complement(5457..5777)
                     /locus_tag="VGA15_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589197.1"
                     /translation="MTGSELKQIRKDLGHAIGRSLSAADMAKLCGLAPGDGPDTIRKW
                     EVKGPSGPAAELLRILAMASDRYPILENFNVFDRFNIDEKDRPARREAFRENMRDEVR
                     RRLG"
     gene            5979..6215
                     /locus_tag="VGA15_15805"
     CDS             5979..6215
                     /locus_tag="VGA15_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589198.1"
                     /translation="MRRLTENLSPADRRFYWKFVGSLFGFYGAVMIIMVGVFFGNHLS
                     RNRALEPVVAETIGEKLPAGIKAPMPLRHAAKYD"
     gene            complement(6308..7093)
                     /locus_tag="VGA15_15810"
     CDS             complement(6308..7093)
                     /locus_tag="VGA15_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9589199.1"
                     /translation="MKSRGIQSIEVGGRLLRALTRIGEPMMLRDLAREAGMTPAKAHP
                     YLVSFSRLGLIERNEVTGRYEVGALALELGLVGLRRLSAVRVAEPKIAALANDVKHTV
                     SLTVWGTHGPTVVRLEEPDHPVHIMMRVGSVMGMLETATGRIFTAFLPQKVAMAALKS
                     GLDHFGVGYNSKRAITGPGIDKMLADIRARHLARAVGDPLPGINVFAAPVFDHEGAVV
                     LSVTAMGPESSFDARWTSAIAKALLDCTNDISRRLGFAASTRK"
     gene            complement(7090..8826)
                     /locus_tag="VGA15_15815"
     CDS             complement(7090..8826)
                     /locus_tag="VGA15_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9589200.1"
                     /translation="MADREPNGVDAFYECDALIVGSGAAGMSAAVTAGHHGLNVLIVE
                     KESCFGGTTARSGGWLWIPGTSLARQWGIVEQPDQARSYLRHEAGNSFDAARVDAFLS
                     AGPEAVDFFTSQTSLRFDMPLVFPDYHAEAPGGTQGGRSMVTRPFDGRELGPHIKNLG
                     AALPELTVFGMMLGSGKEIVHFMRATRSLASALYVAKRLSRHLMDVMRHGRGMTLTNG
                     NALAGRLAKSAFDLKIPLWLSSPVRELIVENGAVRGAMVEREGRPVRVHAKRGVILAC
                     GGFPRDVTRRKAMFPHAPTGIEHYSPAPTGNTGDGLRMAEAIGGRVEDTLPNAAAWVP
                     VSVTTRKDGSSGIMPHFIDRAKPGVIAVLRNGARFANEGNSYHDFVQEMMKAAKPGEE
                     IAAFLICDHRTLRKYGLGCVPPFPMPIGRHLGTGYLMRGGTLKALAARAGIDAGGLEA
                     TVTQFNASAAGGQDPAFGKGSRAYNRFQGDALHGPNPCIAPIVRGPFYAIKMVIGDLG
                     TYAGIRTDENARALDATDHAIEGLYAAGNDMASIMGGNYPGAGITLGPALTFGYIAGR
                     HISGGAIPASKP"
     gene            complement(8837..9241)
                     /locus_tag="VGA15_15820"
                     /pseudo
     CDS             complement(8837..9241)
                     /locus_tag="VGA15_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(9536..10189)
                     /locus_tag="VGA15_15825"
     CDS             complement(9536..10189)
                     /locus_tag="VGA15_15825"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9589201.1"
                     /translation="MEPGRRKAKKPTRKTYHHGDLRRQLIAAAERIIVERGVEGFTLR
                     EAARRVGVSPAAPSHHFKDAKGLLTEVALLGFRDFGEALAAADKRGGEDPMRRLYEQG
                     TAYVRFALRYPARFQLMFRIDKHDHTNAEFVRVSQASFRILEDTIRAATGTSPDRELS
                     PDGRGLLMAVWSMVHGYCHLAFGGELSNPARGGGGKNVILESLLPLMLKHLPSPFRK"
     gene            10395..10766
                     /locus_tag="VGA15_15830"
                     /pseudo
     CDS             10395..10766
                     /locus_tag="VGA15_15830"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
     gene            complement(10860..11198)
                     /locus_tag="VGA15_15835"
     CDS             complement(10860..11198)
                     /locus_tag="VGA15_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589202.1"
                     /translation="MVWTAIIPDAFPRYVDTWRMRSDGTYREDGRGVVKGTAIQETLS
                     GRWTVEGARMILRQQGQPFVFDGVVLGGIYAGTPYFNGRAASRFCAAKGERPPERCST
                     GPGVAMTATQ"
     gene            complement(11463..12935)
                     /locus_tag="VGA15_15840"
     CDS             complement(11463..12935)
                     /locus_tag="VGA15_15840"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX9589203.1"
                     /translation="MFGTFSRRHFAKLAGLSAFAMAATPAKPEDSETKPAPDRHAPAS
                     FPGGFLWGTATSAYQIEGAVDEDGRGRSIWDTFSHTPGKIEDHSNADRANDHYHRYKE
                     DVGLIRELGVKAYRFSIAWPRVFPQGTGAPNPKGLDFYDRLLDELLKHGIEPFATLYH
                     WDLPQALQDRVGGWQSSDTSKAFADYAGYVAERLTDRVKNIFTLNEAGRFLNFGYGWG
                     IDAPGLKLPPAELNQARHHVVLAHGLAVQAIRAHGRAGTKVGPAENIAACLPAIDTPE
                     NVRAAEIATRELNAGFLGVILEGKYTDGFLQYAGANAPKFTADELKIISSPNDFVGLN
                     IYAPQFYVVAADRAPGFKVLPFPASFPHMNSEWLRVGPETIYWAPRLAAKVWNIDTIY
                     ISENGTSSADNLAADGNVYDLDRIMYLRNYLTQLQRATSEGVPVRGYFLWSLMDNFEW
                     IFGFEKRFGLYRVDFETQRRVAKLSASFYRDVVARNAIGA"
     gene            complement(13023..>13136)
                     /locus_tag="VGA15_15845"
     CDS             complement(13023..>13136)
                     /locus_tag="VGA15_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX9589204.1"
                     /translation="LAMGAKYFQGVGFVDGESPDTVAFEIDRISAYQIDSY"
BASE COUNT         2429 a         3990 c         4064 g         2653 t
ORIGIN      
        1 gcgcgatggg atcgcagggc gggttctttg aatccgtgag cggtcaacag cacgccgacg
       61 agagatgttg ctgcggacgg caaaatcgtg tggtcctgac gccccgacgc tggcgtcaag
      121 ttcgcggagt tgcgtcggcc caaccgggct tagacaaaac catatccgtt ggcgacggtg
      181 gcaaaagagc ccggtcaccg gggagagcac gacataagcc gttaaaacca ttgcgtgcgg
      241 gaatgccggg cgattccggt gtactcgttg ttactcgtgt gcgttctacc actaccaagt
      301 gcacacgaga ccgcgggtgc agcgggcacc cggcattccc cacgcccttc ttggggcgaa
      361 agataaatgc acaactcggg cgcatcgcgc cgcgagatcg cgaagtcgcg tttggacgtc
      421 attgcgagcg cagcgaagca atccatactt tctctctttg cggctttatg gattgcttcg
      481 cttcgctcgc aatgacggtt tcagcactcc tcatgttttg gctgtttaaa gattgaaatt
      541 gagaacttgc acaagcggca gcgcgctccc cttgtgggag agggtcgggg agagggggca
      601 cggactatcg actggccgta acccctcacc cggatcgcat ctgtcgatgc gacgtagccg
      661 aagcttcgct tcggcgttct ttttaagaac ggccaccgaa ggcggcctat gctctcccac
      721 aaggggagag gtgacaccga gcggggctcg ctcaccaaaa atcaggtggt cctgaaatgc
      781 agcacgccgt cgttttcctt ggcggtcaga cgcccctcga ccagcatgta attgacatgg
      841 gcgatcaact cgccggcggc aaagcccatc tgatgcacgt ccagcacgtg cttgtggaac
      901 accaccggca ccagttcctt ggatgttttg gaaatttccc ggcaggcgtc ggcgatcagc
      961 cggcagcggt cctcgtgatg gtcggccaat tgcttgatgc gcgtcttcaa tccgtaaaac
     1021 ggcacgccgt ggccgggcag caccagcagg tcgtaaggaa gggtggtggt gaggctcgcc
     1081 agcgacgcca aatattcgcc gagcgagttc tggtcgggct cgaccgccca gacgctgaca
     1141 ttgggcgaaa tcttgctcag cacctgatcg gacgacaaga acagtttgtc ggcggcgcaa
     1201 tacagcatca cctgatcgag cgcatgaccg ccgccggtga tgaccttgaa gcgccttgcg
     1261 ccgatggaaa tttcgtcgcc atgcgagatc cggcgatagg acggcggcag gatcgaaacc
     1321 cgcttcaaat aatcctggcc acggccgagc aatttgtcgg tcaggctttc atccatgccg
     1381 tggcgccgga agaacaggcg ttgcgccatc ttgcgctctt cggtgccgcg gttctgatgg
     1441 tagaccgact gcagatattc cacctgcgac atatagagcg ggcagttgaa acgttcgacg
     1501 atccatccgg cgaggccgac gtggtcggga tgcgaatggg tcacgatcag ccgcgtgatc
     1561 ttgacgtggg ccagcggacc ttcgaacagc gcggtccagg ccgcgatcga ttcttcgttg
     1621 ccgaagccgg aatccaccat cgcccagccg tcgccgtcgg cgagcagata gatgttgacg
     1681 tggttgagcc ggaacggcag cgcgaggcgg acccacaata cgccgggcat cacctcgacc
     1741 acctgatcgg ggccgggatg attttcccag gggtagcgca gggcctcggc cgaggactga
     1801 acggtgtctg ttttcgaatg catggtttga tatcgcaaaa aaattgtcgt tatcggctta
     1861 gccgcacaat cggcgcggcg ccagccgaaa acaattgtga ttttggcaaa acaccgggga
     1921 ggcggtgcat agccgtcatt ccggggcgcg cgccagcgcg aacccggaat ccaaggattc
     1981 cccgatgcgc aattgcgcat ctgaggtctg gtgctaccgc accatcccgg agtgaccaag
     2041 tctgtctacg ccgcccggat gttggagagg aacgcgtcga tctccgaacg caggacgtcg
     2101 gtttcgcggc ggagcgcgcc ggacgcgctc agcacctcgc cggcagccga gcccgcttcg
     2161 gccgaggcgg tagagacgcc gacgatgttg ctggaaacct cggacgtgcc gccggcggca
     2221 tgctggatgt tgcgggcgat ctcgcgcgtc gcggtaccct gttcttcgac ggcggcggcg
     2281 atcgcggtgg tcacctcgtt gatttcgccg atggtattgc cgatgttgcg gatcgcgttg
     2341 acggctgttg tcgtcacctg ctgcatgctg gcgatctgcg cgcgaatttc gtcggtggcc
     2401 ttcgcggtct ggttggcgag gctcttcacc tcggaggcca ccaccgcgaa gccgcggccg
     2461 gcatcgccgg cgcgcgcggc ttcaatggtg gcgttgagcg cgagcagatt ggtctgcgag
     2521 gcgatcacct ggatcaggtc gaccacgacg ctgatgcggc tggcgttctc ggcgagaccc
     2581 tgcatggtgg cgtcggtctg gccggcttca tcgaccgcct tgcgggcgat ctccgccgac
     2641 gtgattacct ggcggccgat ttcggtgatc gatgacgata attcctccgt gcccgaggat
     2701 accgtctgca cgttggccga ggtttcctcg gcggcggacg ccaccgcgct caccagcgca
     2761 ctggactggt cggcagtggc cgacatgcgc tgcgccgtgg tctgcatgga atcggctgat
     2821 gtctgcaggc catccagcgc ggcgcggacg gttgcttcga attcgacaat ccgggtctcc
     2881 atgcgggagg cgcgttcggc cttgacgatg cgatccttgt cctgctcggt gctcagcgcg
     2941 cgcgcctcga tcatgctccc gcgaaagatc tccagcgaat tcgccatcgc cgcgatctcg
     3001 tcgcgctggc ggctgcgatg aatttccgta tcgagatcgc cgcccgacag cagctgcatc
     3061 gagcgttgca gattgccgat ccgccgcagg atgttgcggc cgtcatagag ccagacgaac
     3121 agcacggatc cgatgaaagt gaggccgccg agcgcaagca tgaccacggt cgcgaacgaa
     3181 atctcccggc gcgcctgcca ggtcgaggcg tcggtctcgg tctgtacgcc atcgacgagt
     3241 tgcttcacgc tgatgccgag gccgacattg agcttgcggg tttcgtccag aatgagctgg
     3301 ccataatcgt cggcgtcgag ttccttctgc ctggtcttga agacgctggt cttgccgtcg
     3361 ccgaatgcca gcaatttcag cgcggcgtcc ttgagggcgg cggtgtcgtt atttttggtc
     3421 aagctatcga ggttggattt gatgttcgga tatgcggcct tgaacgcctt ctcgacctgt
     3481 tcgagcgcgt cgccgttggt tgccgagagc gccgccatca tatgcaaggc caacagattg
     3541 gtgctggcga tgacgttgcc aagttgctcg atcgcttgcg ctgcttcgat ggcatcgtcc
     3601 tgcgagagaa gattggcgga cccgaggatt gccctgatcc tggtttgcgc atcgatcatc
     3661 gcgggacccg cggcggcggc gaaagccgtc tgggccttgc gcagggcatc gtattgcttc
     3721 tcgcgctggg cagctattct gagccgttcg gtggccgccg cgcccaggct tttgatggtg
     3781 tcgtcgatgc ttttgacggt ttcaccgagt gcggcgacga cggtcttgtc ggctccgagc
     3841 tcggtgatct ggccgagctt gtcgaccgcg tcgtgctggg tctccttcat tttcctggcg
     3901 cgttcgttca gcgcctcttc gctgcccgag gccagcagtg ccggtccctg gctggcaagg
     3961 cttgcgctct gcgccgccaa ttgcaggctg gcggcaagac ggggaatgtc ccgtccgctc
     4021 agatcgagca tcgacccgcc aagatgtccc agcaccatgc cggcgccggc gctgatcacg
     4081 atggccatgc ccgcgatcac agcgaaggcg gcgaacagac tacccctgac gccccatttc
     4141 gggcgccgga agccaaggcc ccgcaatttg cccatcgttg atcgtaacgc catgctttct
     4201 gcctcgcgct cgcttcatcc ccaagatgaa ttcgagcggc agtatcgcgt taccaagtta
     4261 acaaaatcgg gaacttttgc aggatccgcg caaattttgc gcgccgaaag caaaaaggcc
     4321 ggcgcgaggc cggcctttcc gagacttttt tcaatccgtt gcggatcagt acttcgcgac
     4381 caccgggcca ccccagttga agcgatagtt gatgccagcc ttgacggtat gctcgtcgtc
     4441 gcggaaccgg gtgcccacaa tgacggcggg accggtcgtg aacgtggtgc tgccgaaatt
     4501 gtaatactgg tattcgacct tggcggacca gctgggggcg aacatgtatt cgaggccggc
     4561 accaacggtg tagccgtcac gatgattgcc attggtggtg aaagccaccg gcacgccgcc
     4621 cgtggttacg ttgaggttgt taccgtcctt ccaggcataa ccgcccttgg cgtaaagcaa
     4681 cgccgggccc caggtgtagc caagccggcc ggtgaccgat ccaagctgat cgttcttgga
     4741 ggttaccagc acgccgcccg ggaacaccac gccgttgttg ttgttgctca gccagctgta
     4801 ctgggcttcg acgccgacaa cccagctctg ggcgaactga taatcgaatc cgccctgcac
     4861 gccgccgagg aagcggccat cattgccgat caggctgttg ttgccagcga acgcgccgcc
     4921 gacgtggccg ccgatgtaga agccggtcca gttgtagatc ggcgcggcgt aggccggagc
     4981 cttggtgtag gggcgagccg caagatcggc tgccattgct ggcgcggccg cgccgagcgc
     5041 gacgagggcc acagtaccaa gtagaaactt cttcatcagt cttaatctcc aacgatcatt
     5101 ttctaggcgc cgtgtaccgg ccccatccat ggcgggagat agacaggttt tgcacaaaat
     5161 gctgtcaccc ataggtcaca gtcagaaagt tccaacctat tgatggaact acaatttccc
     5221 gtgactaatg gattcctaat ggaacctgaa tcagatctta atttttgggg cttccgcacc
     5281 tggatctggc gtatgtgcac cttaagaagg cttacttcgt tcctgatgca tcccacgcat
     5341 gcaattcgga aggatcgcaa gacattgcgc agcggagcgc cgcgcgaacc gcgaagtgca
     5401 gatgattcgc acggcatgcg aggaaccgca gtgccgggtt cccgacgcgg tgccgatcag
     5461 cccagccggc gccggacctc gtcgcgcatg ttctcgcgga aagcctctct gcgcgcgggg
     5521 cgatccttct cgtcgatatt gaagcggtcg aagacgttga agttttccag gatcggatat
     5581 cgatcgctcg ccatcgccaa tatgcgaagc aactcggccg ccggcccgct ggggcctttg
     5641 acttcccatt tgcgtatcgt gtcggggccg tcgccaggcg caaggccaca cagctttgcc
     5701 atgtcggcgg cggaaaggct tctgccgatc gcatgaccga gatccttgcg aatttgtttt
     5761 agctcagagc cggtcattcg agttgatccc gcgcgtcgcc cgatgatttt cgaatccgat
     5821 gtgtccggca aatcatagcc acgatcggtc ccggtctccc caatattttg atcggaatcg
     5881 gacgccggat cacgatttcg ggtgtgtgaa gccgatcaca gggccttcca ttgggtgcag
     5941 tttatcctgg ctttgcgttc gtccatgagg agccgcacat gcgccgactg accgagaatc
     6001 ttagccccgc ggaccgtcga ttttactgga aatttgtcgg cagcctgttt ggtttttatg
     6061 gtgcggtgat gatcatcatg gtcggcgtgt tcttcggcaa tcatctgtca aggaaccggg
     6121 cgcttgaacc ggtggtggcc gagacgatcg gcgagaagct gccggctggg atcaaagccc
     6181 cgatgcctct gcggcacgcg gcgaaatatg attgatttcc accaggcctt tttcacgtga
     6241 aagagcggga aggggctgac ctgcagggcg gcgacgagac ggggtttcct gatatccggc
     6301 agaggcgcta tttccgggtt gaggccgcaa atcccagccg cctcgagata tcgttggtgc
     6361 aatcgagcag cgcttttgcg atagcgctgg tccagcgtgc gtcgaacgaa ctctcgggac
     6421 ccatcgccgt caccgacagc acgacggcgc cttcatgatc gaacaccggt gccgcgaaga
     6481 cattgattcc gggtagcgga tcgccgaccg cgcgtgccag atgtcgtgca cggatatcgg
     6541 caagcatctt gtcgatgccg ggaccggtta tcgctcgctt gctgttgtaa ccgaccccga
     6601 aatgatcgag acccgacttc agcgcggcca tcgccacttt ttgcggaagg aatgccgtaa
     6661 agattcgacc tgtcgccgtc tccagcattc ccatcacgga tcccacgcgc atcatgatgt
     6721 gaacgggatg gtcgggttcc tcgagcctga ccacagtcgg gccatgcgtg ccccacaccg
     6781 tcagcgagac ggtgtgtttg acgtcgttcg caagcgccgc gatctttggt tcggccaccc
     6841 tgaccgccga tagacggcgc aagccgacaa ggccgagttc gagagcgagc gcgccgacct
     6901 catagcgtcc ggttacctcg ttgcgctcga tcaggccaag gcgcgaaaag ctcaccagat
     6961 aggggtgggc cttggcgggc gtcattcctg cttcgcgggc aagatcgcgc agcatcatcg
     7021 gctcgccgat tcgggtcagc gcacgcagca accggccccc gacttcgatc gactggatgc
     7081 cgcggctttt cacggcttcg atgccgggat agcgccacct gaaatatgcc ggccggcgat
     7141 atagccgaaa gtcagtgcgg gcccgagcgt gatgccggcg ccgggatagt tgccgcccat
     7201 gatgctcgcc atgtcgttgc cggcggcata gagcccttcg atcgcatgat cggtggcatc
     7261 aagtgcgcgg gcgttctcgt cggtcctgat cccggcatag gtgccgagat cgccgatcac
     7321 cattttgatc gcgtaaaagg gtccgcgcac gatgggggcg atgcaggggt tcggtccatg
     7381 cagtgcatcg ccctgaaacc ggttataggc gcgcgacccc ttgccgaaag cgggatcctg
     7441 tccgcccgcc gccgatgcat tgaactgcgt gacggtcgcc tcgagcccgc cggcatcgat
     7501 cccggcccgg gccgcaaggg ctttgagcgt gccgccgcgc atcagatagc cggtgccgag
     7561 atggcgaccg atcggcatcg gaaacggcgg cacgcatccc agaccgtatt tgcgcagggt
     7621 tcgatgatcg cagatcagga aggcggcgat ctcctcgccc ggttttgccg ccttcatcat
     7681 ctcctgaacg aagtcgtgat aggagttgcc ctcgttcgca aagcgcgcgc cattgcgcaa
     7741 gacggcgatc acgccgggtt tcgcgcgatc gatgaaatgg ggcatgatac cgctcgatcc
     7801 gtctttccgc gtcgtgaccg ataccggcac ccatgccgcg gcattcggga gtgtatcttc
     7861 tacccgcccg ccaatggcct cggccatgcg caggccatcg ccggtgttgc ctgtcggcgc
     7921 cggcgaataa tgctcgatgc cggtcggggc atgcggaaac atcgccttgc gcctggtgac
     7981 atcacgcggg aagccgccgc aggccaggat gacaccacgt ttggcatgaa cgcgaaccgg
     8041 gcgcccctcc cgttcgacca tcgcgccgcg caccgcgccg ttctcgacaa tcagttcgcg
     8101 gaccggggac gacagccaca agggtatctt caaatcgaac gcggatttcg caagacgtcc
     8161 ggccagcgca ttgccgttgg tgagcgtcat gccgcgaccg tggcgcatga cgtccatgag
     8221 atgccgggac agtcgcttgg cgacatagag cgccgaggcc agcgatctgg ttgcccgcat
     8281 gaagtggacg atctccttgc ccgaccccag catcatgccg aacacggtca attccggcag
     8341 cgccgcgccg agatttttga tgtgcgggcc gagctcgcgc ccgtcgaagg gccgcgtcac
     8401 catcgagcgg ccgccctgcg ttccgccggg tgcttccgca tgatagtcgg gaaacaccaa
     8461 tggcatgtcg aaacgcaggg aggtctgcga ggtaaagaag tcgacggcct ccggtccggc
     8521 gctcagaaag gcatcgaccc gcgccgcgtc aaaactgtta cccgcttcgt gccgcagata
     8581 gctgcgcgct tgatccggct gttcgacgat cccccattga cgcgccagcg acgttccggg
     8641 aatccagagc cagccgccgg agcgcgccgt ggtcccgccg aaacaggatt ccttctcgac
     8701 gatcaagaca ttgaggccgt ggtgaccggc ggtaaccgcc gccgacatgc cggcggcgcc
     8761 ggagccgacg atcagcgcgt cgcattcgta aaaggcatcc acgccattgg gttcacggtc
     8821 tgccacgcgg gatcccttac ttcttcagaa gcggacattt ggattccgac accggccgat
     8881 acgcgtcttc gcctttgacg gtcttgacga tgtcgaggta atcccagggt tccttggatt
     8941 ccgagggctg cttgacgcgg gcgagataca tgtcgcggat gacgcggccg tccgcgcgca
     9001 gcgtgcctcc atgcacgaag atatcctcga tcggcagttc gcgcattttt gccatcacct
     9061 gtgccggatc gtcggtaccc gccacccgga tcgccttgag ataatgcagc accgagccgt
     9121 agacgccggt gtggatcatt gtcggcatga cgccagtctt ggcgaagaat tttttcgacc
     9181 atgcgcgggt gttgtcgtcc atgttccagt aggaggcggt cgtcatatag gtgcccgacg
     9241 aggatgctgc aggccaaaag gtgtggctcc atcgtttctc tccctttgtc cgctctgcgg
     9301 cggatcagac tgcgcggcat ttcacgcatc ggagggactt tggtaatagc aaactcatct
     9361 gtcaatatag aattggatcg atagcatgcc ggccggcttt gccgccggtc tcatgcgccg
     9421 ttttgcattt gcggtcagcg tgttcgtcgc cattcatctg tcgaagaacc tggcgcttga
     9481 accggccgcg gcaggggcaa tgagcggcaa gccgccgatc aacactgcga agatgtcatt
     9541 tcctgaacgg cgatggcaga tgtttgagca tcagcggcaa aagggactca aggatcacgt
     9601 tcttaccgcc acccccgcgc gcgggattgc tgagctcgcc gccaaacgcg agatggcagt
     9661 acccatgcac catcgaccag acggccatca gcaacccccg cccgtcaggg ctcagttccc
     9721 gatccggtga agtgccggtg gcggcgcgga ttgtgtcttc gagtatgcga aaggacgcct
     9781 gcgagacgcg cacgaattcg gcattggtgt ggtcatgctt atcgattcgg aacatgagct
     9841 ggaaacgcgc cggatatctg agcgcgaacc gtacataagc ggtgccctgc tcgtagagcc
     9901 gtcgcatggg atcctcgccg ccccgcttat cggctgccgc cagtgcttcg ccgaagtcgc
     9961 gaaagccgag cagcgccact tccgtaagca gccccttggc gtccttgaaa tggtgcgatg
    10021 gggcggcggg cgaaacaccg acgcggcgcg ccgcctcgcg cagcgtgaaa ccctccacgc
    10081 cgcgctccac gataatccgc tccgccgcgg cgatgagctg acggcgcagg tcgccgtgat
    10141 gataggtctt cctggtcggc ttcttcgcct tgcgccggcc aggttccatc ttgacactgt
    10201 tcaaatcgag cctaaaccta attgaacact gttaagatca tagcgtgtaa actcatggtt
    10261 gggaagtatc tggcgcggtc cggcctcgta atgatggcta ttctggccgc ggccgtggct
    10321 ctttattcgc ttcgctacta cggcgcgctc gtcgacggct ggctcgacgt tgattctggt
    10381 cggggcgagt ctatgtcgca gcctgcgtgg ttgccggtac tggggggctg gccacttcgc
    10441 cctcagcgtc cggcggcccg gtcgcgggac ttggcttcgg catcctggcg gtgctgtggg
    10501 tcggtacgac gcttggcgcc tggcgcgccg ccgtgcagcg ccaattcgcg ctccatcgtc
    10561 tgttgatgcg cttcagctac gcgatgacct tcggcgcggt gaccttgcgg ctgcaaattc
    10621 cgctcgggtt cgcgctggga tacccgagct attcggcgat gtcggtatgg ctcgcttata
    10681 cctcctggat tccgaacgtg atcgtcgtgg cgctctattc ggtgacggag gcattgcgcc
    10741 gtccggcggc gcccgcgacg gtctgatctc cgggtgcagg caacaccgaa tatgagggct
    10801 ttggtgtgat ggctggcttg caataagtca aaaaggcgaa ccagctctcg tcgttgggac
    10861 tactgcgtgg cagtcatcgc tactccgggc cccgtactgc accgttcggg cggcctttca
    10921 cccttggcgg cgcaaaaccg ggaagcagca cgaccgttga aataaggcgt tccggcatag
    10981 atgcctccca agaccacgcc gtcgaacacg aacggctggc cctgttgccg aagaatcatg
    11041 cgggcccctt caaccgtcca gcgtcccgac agcgtttctt gaatggccgt gcccttcaca
    11101 acacctcgcc cgtcctcgcg ataggttcca tcggatctca tgcgccaggt gtcgacatat
    11161 cgcgggaagg catcggggat gatcgccgtc caaaccacgc catccggtcc ctcggcggcc
    11221 tccagcgaaa aagacggact tgcgtggaaa cagcagagca acgccaacca gaccgcgaac
    11281 agggcaaccg gcgcgccatt gttttactcc gcaatgttca accttacacc ggtaaccggc
    11341 aggtcattag tgtaaccgag cgcggtcggc gttcgaaatg taagcagttt atcaaaactt
    11401 agactgggat cccgatcgcg aggattccgg cgccgtttct aatgtcgaac gaccactttc
    11461 attcaagccc cgatcgcatt gcgcgcgacg acgtcgcgat agaatgacgc gctgagcttt
    11521 gccacccggc gctgcgtctc gaagtccacg cggtagagcc caaaacgctt ttcgaagcca
    11581 aaaatccact cgaaattatc catcaggctc cacaggaaat agccgcgaac cggcacgccc
    11641 tcggaggtcg cgcgctgcaa ctgcgtcaga tagttgcgca ggtacatgat gcgatcgagg
    11701 tcgtaaacgt taccgtcggc cgccagatta tcggccgaag aggtgccgtt ctcgctgatg
    11761 tagattgtat cgatgttcca gacttttgct gccagccgcg gcgcccaata gatggtctcc
    11821 gggccaaccc tgagccactc ggaattcatg tgcggaaacg aggcggggaa gggcagaacc
    11881 ttgaagccag gcgcgcggtc ggctgcgacc acgtaaaatt gcggcgcgta gatgttcagg
    11941 ccgacgaaat cgttcggcga ggaaatgatc ttcagctcgt cggcggtaaa tttgggagca
    12001 ttggcgccgg catattgcaa aaatccgtcg gtgtatttgc cctccaggat cacgcccagg
    12061 aatcccgcgt tcaattcgcg cgtggcgatc tcggccgcgc gaacgttctc gggcgtatcg
    12121 atcgccggca ggcaggccgc gatattttcc gccgggccga ccttggtccc cgcgcgtcca
    12181 tgggcgcgga tcgcctgtac cgccagaccg tgcgccaata cgacgtggtg acgggcctgg
    12241 ttcagttcgg ctggcggcag tttgagaccg ggggcgtcaa tgccccatcc ataaccgaag
    12301 ttcaggaatc ttccggcttc gttgagcgtg aaaatgtttt tgacgcgatc ggtcagacgt
    12361 tccgccacat agcctgcgta atccgcaaac gccttggagg tgtcgctgga ttgccatccg
    12421 ccaacccggt cctgcagcgc ttgcggcaaa tcccaatgat acagcgttgc gaacggttcg
    12481 atgccatgct tcagaagctc atcgagcagg cgatcgtaga agtcgagacc cttgggatta
    12541 ggcgcgcccg ttccttgcgg aaatacccgc ggccacgcga tcgaaaaacg atacgccttg
    12601 acccccagtt ccctaatcag gccgacgtct tccttgtagc gatgatagtg gtcgttggcg
    12661 cggtcggcgt tgctgtgatc ctcgatcttg cctggcgtgt gggagaacgt gtcccagatc
    12721 gagcggccgc ggccgtcttc gtcgaccgcg ccttcgatct gatacgctga tgtggccgtg
    12781 ccccatagaa agccgcccgg aaaactggca ggggcgtgcc ggtcgggtgc cggttttgtc
    12841 tcactgtcct ctggcttcgc gggcgtcgcc gccatcgcga aggcggaaag gccggcaagc
    12901 ttggcaaaat gccggcgcga gaatgtaccg aacatcgact gctcctgtca ggctttctct
    12961 atcgggccta tgctgacgcg ttttcttgac gcgaaccggt ctccacttcg cttgaaaacg
    13021 ctctaataag aatcaatttg ataagccgaa atcctgtcga tctcgaatgc gacggtatcg
    13081 ggagattcgc cgtcgacgaa tccgacgccc tggaagtatt tcgcgcccat cgccag
//
LOCUS       DASWGM010000553         5682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1156435,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000553 DASWGM010000000
VERSION     DASWGM010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156435"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VGA15_15850"
     CDS             complement(<1..642)
                     /locus_tag="VGA15_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9589205.1"
                     /translation="MAIQVRGSPSPVDVAQFIDAQPVGGFQIRLLLTCAAVLFLDGFD
                     TQAIGFVAPALAREWGVSKAALGPVFSAGLFGLMIGALVFGPLADRVGRKKIIVFSTL
                     AFGIGTLVTAFVQDVNMLLAIRFLTGLGLGGAMPNAIAITSEFNPRRRRATMVMIMFC
                     GFSVGAALGGFLAAALIPQFGWRSVFVVGGLAPLLLVPILARRLPESVRFLALT"
     gene            complement(747..1985)
                     /locus_tag="VGA15_15855"
     CDS             complement(747..1985)
                     /locus_tag="VGA15_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEX9589206.1"
                     /translation="MDDIIIVGAGVGGLTLGLALHAAGIPCRIFESAAEIRPIGVGIN
                     LLPHATNELAALGLEAELARVAIATEDATFFNRFGQLIYSEPLGRAAGYEHPQFSIHR
                     GDLQMVLLEAFRARAGHNRLNTNHHCTGVEQNETGVSVTFSDGPEGTSRSTMRGRAAI
                     ACDGINSAIRKQLYPGEGEPRYSGVNMWRGVTRWKPMLSGASMVRAGWLSHGKMVIYP
                     IRQASGADGLQLINWVAEIETATYRKRDWTRPGRIEDFIGAFADWHFDWLDVPAFIRA
                     ADSVLEFPMVDQDPLPRWSFGRVTLLGDAAHPMVPRGSNGAGQAILDARALTSALLEH
                     SDSVAALAAYEKERLEATTRIVLTNRTNPPDAILREVFQRTNDQPFAAIDDVISRDEL
                     VALSEGYKRIAGYSKDALRA"
     gene            complement(2178..3725)
                     /locus_tag="VGA15_15860"
     CDS             complement(2178..3725)
                     /locus_tag="VGA15_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589207.1"
                     /translation="MRILVTILVLLCGVLAARAGQSALERGTAIIDPDGLRELDRGRL
                     GLSGVILPMRSSNAPLTSSQLFALPSMVPVRKALDDEFDRYIAHHKANLPNETIGVGT
                     AFDFQLFDRTQLYSRDSRFVLAGIVNRMDRAYLSAANCGEIRLIYRLVRTDAPAVGEN
                     AVSPRLPMTLNIVLKAKGDNAIDASGAAITCSEIARRWLAAGDSELTGAELAEKWLSK
                     DGPLALIRPENIDRIETNLQIAHAPKSAVRDFRTDYLMKVFDYKSQSGTFEEAPLENQ
                     IDRERILGDQVLKRDFKAWLLDSAHFAELDRGTILIPEKFLARRAIAPTPVGFGTSDL
                     QPAFGLVEGDDATPDPLFKEADVVAALKQAADAGVTLQNIRSAAGLERRLNDITCGGC
                     HQTRGIGGFHFPGVDWMAAKPSNSTVVPASPHFFGDQIRRRDILTSLRGGTQPDYSRG
                     FSSRPQLRGSIELAGTSFEDGWGALCYQHKLEAADNDASFQSWRCAEGLTCQPVDRAS
                     RIGMCFR"
     gene            complement(3909..4892)
                     /locus_tag="VGA15_15865"
     CDS             complement(3909..4892)
                     /locus_tag="VGA15_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589208.1"
                     /translation="MSEQMAEQTSGESGSLKAALSSLETPSTPFAVSAKESVPETTNV
                     DSPKLSPEQGGAAETPKMGAPEVDAQKPDAAKPDAAKPDAAKADAPRMPGNITIMSTG
                     DRAGSAPEAEQGSGMFGKRRLAALAAVAALAAIAGALGGALATAGVGHFLGEGATASA
                     GPALEASVARIDADILALMANLENISKTSAAQFNKASDRLDKVEKAQAEPVAKLAKLS
                     EAVDKLRGTPVAAAAAAATPAAAKEVTGSISAPATTPAAAPKPEVARLPTVEGWVLRN
                     VANGAALIEGRRGMYEVYAGDPIPGLGRVDAIRRQDGHWVVVTGKGLIVAR"
     gene            5132..5503
                     /locus_tag="VGA15_15870"
     CDS             5132..5503
                     /locus_tag="VGA15_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9589209.1"
                     /translation="MAIEKCINEYDVDDVIFEEGSTGRELFVVLDGIIDIVKINGTSK
                     TLIVTLGKGEFFGEMAVIDGSSRSATAIASAPNTRVMRINHARFVYLVSQQPAFALMI
                     MDALSKRLRASNDVIFGAANA"
     gene            5500..>5682
                     /locus_tag="VGA15_15875"
     CDS             5500..>5682
                     /locus_tag="VGA15_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9589210.1"
                     /translation="MNERKPSPFPVLLNNDVCSLIQAAEHVYQIRFKNRAANAYLVRG
                     KSRTIMIDAGLSSNFPH"
BASE COUNT         1050 a         1799 c         1814 g         1019 t
ORIGIN      
        1 ggtaagcgcg aggaaccgca ccgattccgg cagccgccgc gccaggatcg gaaccagcag
       61 cagcggggcg agcccgccca ccacgaacac cgagcgccag ccgaattgcg ggatcaacgc
      121 cgccgcgaga aagccgccga gcgcggcccc caccgagaac ccgcaaaaca tgatcatcac
      181 catggtggcg cggcggcgac gcggattgaa ctcggaggtt atcgcgatcg cattcggcat
      241 cgccccgcca agaccaagcc cggtcagaaa gcggatcgcg agcagcatat tgacgtcctg
      301 gacgaacgcg gtgaccagcg tgccgatgcc gaacgccagg gtcgagaaca cgatgatctt
      361 cttgcgcccg acccggtcgg caagcggccc gaacacgagc gcgccgatca tcagtccgaa
      421 caggccggcg ctgaacaccg gaccgagcgc ggccttgctc acgccccatt ccctggcgag
      481 cgccggcgcg acaaagccga tggcctgggt gtcgaagcca tcgaggaaca gcacggccgc
      541 gcaggtcagc aacaatcgga tctgaaatcc gccgaccggc tgcgcatcga tgaactgggc
      601 gacatcgacc ggcgatgggc tgcctcgaac ctgaatcgcc atcgttgtcc ctctctgaaa
      661 cgtcggaccg cggttcttct cgaccggtat tcctgacgcg atagtctggc gtgaattcgg
      721 caagagggga aggataccct gccccgtcag gcgcgcaatg catccttcga atagccggcg
      781 atccgcttgt aaccttccga caacgcgacc agttcgtcgc ggctgatgac atcgtcgatg
      841 gcggcgaaag gctggtcgtt ggtgcgctgg aacacctcgc gcaggatcgc gtcgggcgga
      901 ttggtgcggt tggtcagaac gatgcgggtg gtggcttcga gccgctcttt ctcatacgcc
      961 gcgagggctg caacggaatc gctgtgttcg agcagcgccg aggtgagcgc gcgcgcgtcc
     1021 agaatcgcct ggccggcgcc gttggagccg cgcggcacca tcgggtgggc ggcgtcgccg
     1081 agcagcgtga cccggccgaa actccagcgc ggcagcgggt cctggtcgac catcgggaat
     1141 tccagcacgc tgtcagcggc acggatgaac gccggcacat cgagccagtc gaaatgccag
     1201 tcggcgaagg cgccgatgaa atcctcgatc ctccccggcc gggtccagtc gcgcttgcga
     1261 taggtcgcgg tctcgatttc ggccacccag ttgatgagct gcaatccatc cgcgccggaa
     1321 gcctgacgga tcgggtaaat caccatcttg ccgtgcgaca gccatcccgc ccgcaccatg
     1381 ctggcgcccg acagcatcgg cttccatcgc gtcaccccgc gccacatgtt gacgccggaa
     1441 tagcgcggct cgccttcgcc ggggtaaagc tgcttgcgga tcgcggaatt gatgccgtcg
     1501 caggcgatcg cggcgcggcc gcgcatggtg ctgcggctgg tgccttcagg cccatcggag
     1561 aaggtgacgc tgacgccggt ctcattctgt tcgacgccgg tgcaatggtg gttggtgttt
     1621 agccggttgt gtccggcgcg cgcgcggaag gcctcgagca gaaccatctg caggtcgccg
     1681 cgatggatgg agaattgcgg atgctcgtaa cccgcggccc ggccgagcgg ctcggaatag
     1741 atcagctggc cgaagcgatt gaagaaggtc gcgtcctcgg tggcgatcgc gacccgcgcc
     1801 agttccgctt caagtccgag cgcggccaat tcattggtgg catgcggaag caggttgatg
     1861 ccgaccccga tcggcctgat ttccgccgcc gattcaaata tccggcaggg gattccagcg
     1921 gcgtgcagcg caagcccaag ggtcaatccg ccgacgccgg ccccgacgat aatgatgtcg
     1981 tccatgtctg ctcccggcgc gcgctgtcag gcgattgccg ggatgaaaca cgattggtga
     2041 gcctactgtc aatccaggga aatgcgtgca atccgggggc tatctcgcgt acagcatctg
     2101 tgtggatccc ggctctgcgg agcagcgtga agaacgctgc accgcgtccg ggacacgagg
     2161 tttcggttag cggtctttta gcgaaaacac atcccgattc gagaagccct gtcgacgggc
     2221 tggcaggtga gcccctcggc gcagcgccat gactggaagc tcgcgtcatt gtcggcggcc
     2281 tctagcttgt gctggtaaca cagcgcgccc cagccgtctt cgaatgaggt cccggcaagc
     2341 tcgatgctgc cgcgcaattg cgggcggctg gaaaaacccc gcgagtaatc cggctgcgtg
     2401 ccgccgcgca gcgaggtcag aatatcgcgg cggcggatct ggtcgccgaa gaaatgcggc
     2461 gaggccggca ccacggtcga attggagggc ttggccgcca tccagtcgac gccgggaaaa
     2521 tgaaagccgc cgatgccgcg ggtctgatgg cagccgccgc aggtgatgtc gttgagccgg
     2581 cgctcaaggc cggcagccga acggatgttt tgcaaggtca cgccggcatc cgccgcctgc
     2641 ttcaaggctg cgaccacgtc agcttccttg aacagggggt caggcgttgc atcatcaccc
     2701 tccaccaagc caaaggccgg ctgcaggtct gaggtgccaa atccgaccgg cgtcggcgcg
     2761 attgcgcggc gggcgaggaa tttttcgggg atcaaaatcg tgccgcgatc cagttcggca
     2821 aaatgcgcgg aatcgagcag ccacgccttg aagtcgcgct tgagcacttg atcgccaaga
     2881 atccgctcgc gatcaatctg gttctcgagc ggcgcttcct cgaaagtgcc ggactgtgat
     2941 ttgtaatcaa acaccttcat gaggtagtcg gtgcgaaaat cacggaccgc cgattttggc
     3001 gcgtgcgcga tctgaagatt ggtttcaatg cggtcgatgt tttcaggccg gatcagagcc
     3061 agcgggccat ccttcgacaa ccatttttcc gcaagctccg cgccggtgag ttcggaatcg
     3121 cctgcggcaa gccatcgccg cgcgatctcc gaacaggtga tcgccgcgcc gcttgcgtcg
     3181 attgcgttgt cgcctttggc cttcagcacg atgttgagcg tcatcggcag ccgcggcgac
     3241 accgcatttt cgccgaccgc cggcgcgtcc gtccgcacca ggcgatagat cagccggatc
     3301 tcgccgcaat tcgctgccga aagataggcg cggtccatcc ggttgacgat acccgccagc
     3361 acgaagcgtg aatcgcggga gtaaagctgt gtgcgatcga acagttgaaa atcgaaagcc
     3421 gtgccaaccc cgatggtttc attcggaaga ttggccttgt gatgggcgat gtaacgatcg
     3481 aactcgtcgt cgagcgcctt gcgcaccgga accatcgacg gcaacgcgaa taactggctg
     3541 ctggtgagcg gcgcgttgga cgatcgcatc ggcaggatga cgccgctcag gccaagcctt
     3601 ccgcgatcga gttcgcgcaa gccatcggga tcgatgatcg cggtgccgcg ctcaagcgcg
     3661 ctctgcccgg cgcgcgccgc caagacgccg cacagcaaga caaggattgt gacaaggatt
     3721 cgcatgcgcg gcccatatcc tgacgaacgc cgcgcatcaa ggacgccaat tcaaggcgtc
     3781 cttgcgggca atgtcttcct agtcaggaaa ttagccgaaa agcagacggg ctgtcgcgca
     3841 aatccggacc tgtccggcca tcgcttcacg tcgtggtgaa gcggtcgggg gtgcgaagcg
     3901 atcatgcgct agcgcgcgac gatcaggcct ttgccggtca cgaccaccca gtggccgtcc
     3961 tgacgcctga tggcgtcgac ccggccgagg cccggtatcg gatcgccggc atacacttcg
     4021 tacatccccc ggcggccttc gatcagggca gcgccattgg cgacgttgcg cagcacccag
     4081 ccttccaccg tcggcagtct cgccacctcg ggtttgggcg cggcggcggg cgttgtcgct
     4141 ggcgctgaga tcgagccggt gacttccttc gccgcggctg gcgttgccgc ggcggcggcc
     4201 gcagcaaccg gcgtaccgcg gagcttatcg acggcctcgc tgagcttggc gagtttggca
     4261 accggttcgg cttgcgcctt ctcgaccttg tcgaggcgat cgctggcctt gttgaactgg
     4321 gccgcgctgg ttttggagat attttcgagg ttggccatca gcgccaggat gtcggcgtcg
     4381 atccgcgcaa ccgacgcttc gagcgcggga ccagctgacg ccgtggcacc ttcgccaaga
     4441 aaatgcccga cacccgcggt cgccagtgcg ccgccgagtg cgcctgctat cgctgccagc
     4501 gccgccacgg ccgccagcgc tgcgagccgg cgcttgccga acatcccgga gccctgctcg
     4561 gcctccggcg cggaaccggc gcggtcgccg gttgacatga tcgtgatgtt gcccggcatg
     4621 cgcggcgcgt cggccttcgc agcgtcaggc ttggcagcat caggcttggc agcatcaggc
     4681 ttttgagcat ccacctcggg cgcgcccatc ttgggggttt cggcggctcc gccctgctcg
     4741 ggcgacagct tcggagaatc gacattggtg gtttccggca ccgattcctt cgccgacacc
     4801 gcgaacggcg tgctcggcgt ctccaaggaa ctgagagcgg ccttgaggga accggattcg
     4861 ccgctggttt gctcggccat ttgctcgctc atgttcaaaa cctccagtaa aagttgacca
     4921 tttggtaacc tttaatttct tgccaaatcg ttaccgggtg ccgagttacc aaaattttag
     4981 ggaattatcc ggccgctttt cgatcccgtc aggattgcca cttggcctat gctgccctgc
     5041 ggtaatgggc ggtgatccga tgcgtcgttt gcgcggccct gtttcccgac taacatggca
     5101 acgcggacca gccagaaggc gggggagttc catggccatc gagaaatgca tcaacgaata
     5161 tgacgtggac gatgtcattt tcgaggaagg ctcgaccggg cgcgaactgt tcgtggtgct
     5221 cgatggtata atcgacatcg tcaaaatcaa cggcaccagc aagacgttga tcgtgacctt
     5281 gggcaagggt gaattcttcg gcgagatggc ggtgatcgac ggctcgtccc gctcggcaac
     5341 cgcgatcgcc tcggcaccca acacccgcgt gatgcggatc aatcatgccc ggttcgtcta
     5401 tctggtcagt cagcagccgg cgttcgcgct gatgatcatg gatgcgctca gcaagcgact
     5461 gcgcgcgtcc aacgacgtca tttttggagc tgccaacgca tgaacgaacg caagccgagc
     5521 ccgtttccgg tcctcctgaa caatgacgtc tgctcgctga tccaggcggc cgagcacgtc
     5581 taccagatcc gtttcaagaa ccgcgccgcg aacgcctatc tggtgcgcgg aaaatcccga
     5641 accatcatga tcgacgccgg gctgtcctcc aactttccgc at
//
LOCUS       DASWGM010000554         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2560367,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000554 DASWGM010000000
VERSION     DASWGM010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2560367"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VGA15_15880"
     CDS             complement(<1..1471)
                     /locus_tag="VGA15_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9589211.1"
                     /translation="MLQGLASLGNAYLSLLNPTSIAFGLCGALLGILVGCMPGLSATL
                     CIALLTTLTVKLPANDAILILVCSYVGTLYGGSRSAILLNIPGTAANAASCADGHALA
                     LRGEAGRAIGIATSGAFCGTLFGVVCLAAFTPTLANVALSFGAYEFFWLALFGVTMSG
                     TLVGNDPLKGWLMGALGLFVAQIGQEGLYAYDRFTFGLPELSGGIALIPALIGAFGFA
                     EVLTTLANPVEHKLLEMRDSIRPRFDEVARYWRTVLRSGAIGVLTGLLPGVGEDSGAW
                     MSYAAAKATTKEREQFGKGSIDGLMAAETGDMSSIPGHMIPMLALGVPGSAPSAVLMA
                     AIIIHGIQPGPMLMVEHRQFVYDMVAMTTLATVCILIFGLYLVRPLLYVLRVKHSVLM
                     PIIFLMSTVGAYATAWRLFDVYSMIAVGIGAFFLRRKGYQMAPFVLGLVLGTLLDKSL
                     RRGLVLSDGSILPFFTRPICIGFASVTIFSMLLYIPAFSR"
     gene            complement(1496..2053)
                     /locus_tag="VGA15_15885"
     CDS             complement(1496..2053)
                     /locus_tag="VGA15_15885"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9589212.1"
                     /translation="MQTSEPEPGAGGAAQSAARLVTPRSDFLGGLAWIAFGTAVAVGS
                     WRMDRLENQDVNPYTVPGLLPGLLGLAIGFFGTLLLARAWQQGALGGKSPRSAAMSAA
                     ERKRFLIVLGLCLTFGIGLVGHGLPFWLAAAAFVSTAIAVLQYPQRRAANQVARGLTI
                     AIVIGLGAGLLITLVFQEFFLVRLP"
     gene            complement(2108..>3207)
                     /locus_tag="VGA15_15890"
     CDS             complement(2108..>3207)
                     /locus_tag="VGA15_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9589213.1"
                     /translation="KSRLLAWHVGSILPGGSRRGSQAALDGAMTQIRVRNAMLLSLFA
                     AAFAAPPALGAEWKPTRPINLIVPWAAGGSTDQITRITATQLEPLLGQTIVIINQAGA
                     SGSLGTRSALDAARDGYTWTSGAAQDLGAYEALGLLKTRITDWNLFLNVANIQLLSVN
                     ADSPYNTAQELIDAMKLKPGQISVATAGVTSGGHNAMEAIAKATGVKYRHVTYDGGNP
                     AVVATVAGETEVTTQLAVEQADMIRGKRLRPLATVSDKPLELAGYGTIPPLASILPGF
                     RMPTNYFGIFIPRGVPDEVVQAVEKAWTQDIVNNDVLKKYATSRGALFAPLYGEQAQA
                     AVFPAVQANAWLLFETNKAKVSPDTVGIPKP"
BASE COUNT          640 a         1023 c         1020 g          524 t
ORIGIN      
        1 cgcgactgaa tgccggaata tacagcaaca tgctgaagat cgtcaccgac gcaaaaccga
       61 tgcagatcgg tctggtgaaa aacgggagga tgctgccgtc ggaaagcacc agtccgcgcc
      121 gcaggctctt gtcgagcagg gtaccgagga ccaatcccaa cacgaacggc gccatttgat
      181 agcccttacg gcgcaggaag aacgcgccga tgcccacggc gatcatcgag tatacgtcga
      241 acaatcgcca cgccgtcgcg tacgcgccga cggtcgacat caggaagatg atcggcatca
      301 gcacgctgtg cttgaccctc aggacataaa gcagcggccg gacaagatag aggccgaaga
      361 tgaggatgca gaccgtggca agtgtcgtca tcgcaaccat gtcgtagacg aactgccgat
      421 gctctaccat cagcatcgga ccgggctgga tgccgtggat gatgatcgcc gccatcagta
      481 ccgcggacgg cgcggacccg ggaacgccca gcgcgagcat gggaatcatg tgcccgggaa
      541 tcgaggacat gtcaccggtt tccgccgcca tcagtccgtc gatggagccc ttgccgaact
      601 gttcgcgctc cttcgtcgtc gcctttgcgg ccgcgtacga catccatgcc ccggaatcct
      661 cgccgacccc gggcagcaag cccgtcaaca cgccgatggc cccggaacgc agcaccgtgc
      721 gccagtagcg cgcgacctcg tcgaagcgcg gccggatcga gtcgcgcatt tccagcagct
      781 tgtgttccac gggattggcc agcgtggtca gcacttcggc aaagccgaac gcgccgatga
      841 gtgccgggat cagcgcgatc ccgccggaca gttccggcaa gccgaaagtg aaccggtcgt
      901 atgcatacag cccctcctgt ccgatctgcg ccacgaacag accgagcgct cccatcaacc
      961 atcccttcag cggatcgttg ccgaccaggg tccccgacat cgtcacgccg aacagggcga
     1021 gccagaaaaa ctcgtaggcg ccgaaggaga gggccacgtt ggcgagtgtc ggggtgaacg
     1081 ccgccaggca caccacgccg aacaacgtgc cgcaaaacgc gccggacgtg gcaatgccga
     1141 tcgcgcgtcc ggcctcgccg cgcaacgcca gcgcatggcc gtccgcacag gacgcggcgt
     1201 ttgccgcggt tcccggaatg ttgagcagga tcgccgaacg cgagccgccg tacagcgtgc
     1261 cgacgtagga gcacaccaga atgaggattg catcgttcgc gggcagtttg accgtaagcg
     1321 tagtcagcaa ggcgatgcac agcgtggcgg aaagtccggg catgcagccg accaggatgc
     1381 cgagcaatgc gccgcacagg ccgaaggcga tcgacgtagg attgagaagc gagagataag
     1441 cattaccgag agaggccaaa ccttgcagca tgacgagttc cgctcgatca cgtcgtcagg
     1501 gaaggcgcac caggaagaac tcttggaaca cgagtgtaat cagcaggccg gcgccgagcc
     1561 cgatcacgat cgcaatggtg aggccgcgcg ctacctgatt ggcggcgcgg cgctgcgggt
     1621 actgcaaaac agcgatcgcg gtcgagacga aagcggcggc tgcgagccag aacggcaacc
     1681 cgtgccccac caagccgatg ccgaaggtca ggcaaagacc cagcacgatc aagaagcgct
     1741 tgcgttccgc cgcagacatc gcggccgacc gagggctttt cccgccgagc gcgccctgtt
     1801 gccacgcccg cgccagcaac agcgtgccga aaaacccgat ggccagacca agcagtccgg
     1861 gcagaagacc cggcacggtg tacgggttga cgtcctgatt ttccaggcgg tccattcgcc
     1921 acgatccgac ggcgaccgcc gtgccgaacg caatccacgc gagcccgcca aggaaatccg
     1981 atcgcggcgt taccaggcgc gcggccgatt gcgccgcgcc accggcgccc ggttctggtt
     2041 cggaggtctg catcgttcga tcgcgcctcc cggtcacgcg gccacgccgg gtccgctgac
     2101 cgcggtatca gggctttgga atgccgaccg tatcgggcga gactttcgcc ttgttggtct
     2161 caaacagcag ccaagcgttc gcctgaacgg ccgggaacac ggccgcttgc gcttgctcgc
     2221 cgtagagcgg cgcaaatagc gcgccgcgac ttgttgcgta tttcttcaag acgtcgttgt
     2281 tgacgatatc ttgcgtccag gctttttcga cggcttgcac cacttcgtcg ggcacgcctc
     2341 tgggaatgaa gatgccgaag taattggtag gcatgcggaa tcccggcagg atcgacgcaa
     2401 gcggcggaat ggtgccgtag cctgcgagtt ccagcggttt gtcgctcacg gtagcgagag
     2461 gacgcagacg cttgccacgg atcatatccg cctgctccac cgcgagctgt gtcgtcactt
     2521 ccgtttcgcc cgcgaccgtc gccaccaccg ctggattgcc gccgtcatag gtcacgtgcc
     2581 ggtatttcac gccggttgcc ttcgcgatcg cttccatcgc gttgtgcccg cccgacgtca
     2641 cgcctgccgt ggcgaccgaa atctggccgg gcttgagttt catcgcgtcg atcagctcct
     2701 gcgcggtgtt gtaaggtgaa tctgcgttga cgctgagcag ctggatattg gccacgttga
     2761 ggaacaggtt ccagtcggtg atccgggtct tgagcaggcc caaggcttcg taggcgccca
     2821 agtcctgagc cgcgcccgag gtccaggtat agccgtccct ggcggcatcg agcgcgctcc
     2881 tggtccccaa cgagcccgag gcgcccgcct gattgatgat gacgatcgtc tggccgagca
     2941 gcggttcgag ttgggtcgcg gtgatgcggg tgatctgatc ggtcgagccg ccggccgccc
     3001 agggcacgat gagattgatg ggacgggtag gcttccattc ggcgcccagt gcgggaggcg
     3061 ccgcgaaggc tgcggcaaaa agcgaaagga gcatcgcgtt acggacgcgg atttgtgtca
     3121 tggcgccatc cagtgcagcc tgtgatcccc ttcggctgcc gcccggcaat atgctgccaa
     3181 cgtgccacgc tagtaaacgg ctttttt
//
LOCUS       DASWGM010000555         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_320333,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000555 DASWGM010000000
VERSION     DASWGM010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320333"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VGA15_15895"
     CDS             <1..1181
                     /locus_tag="VGA15_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX9589214.1"
                     /translation="LYVGVKKVEFLESARSTFWTIIYSTLAISSLAMLVSYLVVRSAI
                     VRRLKASVAVMNRLARGDLSADTPEPRRSDEIGEIMMALVAFKATGLEVERMRADRLV
                     TDQHAAEQRKADMNRLADEFEGAVGQIVETVSSASTELETSAGTLTSTAERAQEITTM
                     VAAASEEASTNVQSVASATEEMASSVNEISRQVQESARMAGEAVDQARTTNDRVSELS
                     KAAARIGDVVELISTIAGQTNLLALNATIEAARAGESGRGFAVVASEVKALAEQTAKA
                     TGEIGQQISGIQAATQESVNAIKEISGTIEKLSEISSAIAAAVEEQGAATQEISRNVQ
                     QAAHGTQQVSSNITDVQRSASETGSASSLVHSAARSLSGESNRLKLEVGKFLNSVRAA
                     "
     gene            1224..>2720
                     /locus_tag="VGA15_15900"
     CDS             1224..>2720
                     /locus_tag="VGA15_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9589215.1"
                     /translation="MNLRIRTILPLIISALASLLVVSAGLTAYDAIGRRQDTQAFLKV
                     NQISQLLLLSAGQWAIERGMTNASLKSADVLSPERRAEIVKLRASADQAFRDAVQRLG
                     AIPAMKAGEKDITDAERIFREFEAFRAKVDENLARPGAERTPEIVQGFFPALTNLILV
                     AGNRLRLTLETLNSPPSVTLAQLVGLRHLTAQMAENAGRERGFLGGIIGARSKLATEG
                     ARTVAGFRGQVELAWETIVPVRRRADTPAKIVAAIAGVESEYFGKYGEIRDAVFAAGN
                     TGEYKVTGSEYVARATTAINSILHLANEIGNASNDEANDEASRSTSSLTLNAAMLFAG
                     IVLAVISFWVAFRRILGPLNAMTGAMTRLAGGDKTIDIPGMNNQDEIGEMGATVQVFK
                     DNMIETDRLRAEQSDVEQRQLQQRKAEMHQLAAAFEGAVSEIVENVSSAATELEASAS
                     TLTSTAERAQELATAVAAASEEASTNVQSVASASEEMTSSVNEISRQVQ"
BASE COUNT          576 a          815 c          865 g          464 t
ORIGIN      
        1 ttctctatgt cggagtgaag aaggtggaat ttctggagtc tgcccgcagc acgttctgga
       61 cgatcattta ttcgactttg gcgatcagct cgctcgcaat gctggtgagt taccttgtgg
      121 tacgtagcgc gatcgtgcgt cgcttgaagg ccagcgtcgc ggtgatgaat cggctggcgc
      181 ggggcgatct ttcggcggac acgcctgagc cgcgacgcag cgatgagatc ggcgagatca
      241 tgatggcgtt ggtcgcattc aaggcgaccg ggcttgaggt cgagcggatg cgcgccgacc
      301 ggctggtgac agatcagcac gccgccgagc aacgcaaggc ggacatgaac cggctcgcgg
      361 acgaattcga gggtgcggtc ggccagatcg tcgagaccgt gtcgtcggct tcaaccgaac
      421 ttgaaacctc cgccggtacg ttgacgtcaa ccgcggagcg ggcgcaggaa atcaccacca
      481 tggtcgccgc ggcgtccgag gaagcctcca ccaatgtgca gtcggtagcc tccgcgaccg
      541 aggaaatggc gtcgtcggtc aacgagatca gccgccaggt ccaggaatcg gcccggatgg
      601 ccggcgaggc ggtcgatcag gcccgcacca ccaacgatcg ggtcagcgaa ttgtcgaagg
      661 cggccgcgcg catcggcgat gtggtcgagt tgatcagcac catcgccgga caaaccaatc
      721 tgctcgcctt gaacgccacc attgaggcgg cccgcgccgg cgaatccggc cgtggctttg
      781 cggtcgtggc ttccgaggtg aaggcgctgg ccgagcagac cgcgaaagcc accggcgaga
      841 tcggtcagca gatcagcggc atccaggcgg caacccagga gtcggtcaac gccatcaagg
      901 agatcagcgg caccatcgaa aaattgtccg aaatatcgtc ggccatcgcg gcggcggtgg
      961 aggagcaggg tgcggcgacc caggaaattt cccgcaatgt gcagcaggcg gcccacggca
     1021 cccagcaggt ctcctccaac atcaccgacg tgcagcgcag cgccagcgag accggatcgg
     1081 cctcgtcgct ggttcactcc gcggcgcggt cgctatccgg cgaaagcaac cgcctcaagc
     1141 tcgaagtcgg caagttcctc aattcggtaa gggcggcctg acgatcttcg aacgcacgaa
     1201 ccgcagcaga aggaaatggc caagtgaatc ttagaatccg cacgattctg ccgttgatca
     1261 tctccgccct ggcctcgctg ctggtagttt ccgccgggct caccgcttat gacgcgatcg
     1321 gtaggcggca agacacccaa gccttcctca aggtcaatca aatctcgcaa ttgctgttgc
     1381 ttagcgcagg tcaatgggca attgagcgcg ggatgacaaa cgcgtcgctc aaatcggccg
     1441 atgtgctgtc gccggagcgt cgtgccgaga ttgtaaagtt gcgagcttct gccgaccagg
     1501 ccttccgcga cgcggtgcag cgcctgggcg ccattccggc gatgaaagcc ggcgagaagg
     1561 acatcaccga cgccgaacgt atttttcgcg agttcgaggc gttccgcgcc aaggtcgatg
     1621 aaaacctcgc caggcccggt gccgaacgga cacctgaaat cgtgcagggc tttttccccg
     1681 ccctgaccaa tctgattttg gttgccggaa acaggctgcg attaacgctt gagacgctga
     1741 atagcccgcc ttcggtcacg cttgcgcaac tcgtcggtct tcgtcacctg accgcgcaaa
     1801 tggcagagaa tgccggcagg gaacgtgggt tcctcggcgg catcataggc gcacgcagca
     1861 agctcgccac cgagggggca cggaccgttg ccggtttccg cggccaggtc gagcttgcct
     1921 gggaaacgat cgtgccagtc cgccggcgcg ccgatacccc ggcaaagatc gtcgctgcga
     1981 tcgcgggcgt cgagagcgag tattttggga agtatggcga aatccgcgac gcagttttcg
     2041 cagccggcaa caccggagaa tataaggtca ccggcagcga gtacgtcgcc cgcgcaacga
     2101 ccgctatcaa ttcgatcctc catctcgcta acgagatagg caatgcgtca aatgatgagg
     2161 cgaatgacga ggcgtcgaga agcacatcga gcctcacgct caacgcggcg atgctgttcg
     2221 ccggcatagt actcgcagtc atcagcttct gggttgcgtt ccgccgtatt ctcggaccgt
     2281 tgaacgccat gacgggtgcg atgacaagac tagccggcgg cgacaagacg atagatatcc
     2341 cgggaatgaa caaccaggac gaaatcggcg aaatgggtgc caccgttcag gtattcaagg
     2401 acaacatgat cgaaaccgat cggctgcgcg ccgagcaatc ggacgtcgag caacggcaat
     2461 tgcagcagcg caaggcggaa atgcaccagc ttgccgctgc attcgaaggc gcggtgagcg
     2521 agatcgtcga aaacgtgtcc tcggctgcga cggagcttga agcttctgcc agtacgctga
     2581 cctcgactgc cgaacgggca caagagctcg caaccgcggt tgcggcagcg tccgaagaag
     2641 cctctaccaa tgtgcagtcg gtggcctccg cgagcgagga gatgacctcg tcggtcaacg
     2701 agatcagccg ccaggttcag
//
LOCUS       DASWGM010000556         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_320334,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000556 DASWGM010000000
VERSION     DASWGM010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320334"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGA15_15905"
     CDS             <1..228
                     /locus_tag="VGA15_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9589216.1"
                     /translation="AVEEQGAATQEISRNVQQAAHGTQQVSANITDVQRGASETGSAS
                     SQVLSAAKSLSGDSNRLKLEVGKFLNSVRAA"
     gene            401..2404
                     /locus_tag="VGA15_15910"
     CDS             401..2404
                     /locus_tag="VGA15_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9589217.1"
                     /translation="MSFLNLRITGRLYGGFGALLLFCAALAGFAVWQLSAIHTQVGSL
                     TLQSKNAIRVGEITTELQATRRSLLRYAFDQDEASFAEAEKRLSKTAELLEVAAKTTM
                     TEERRAAYKAALPEITELKTKRLALGEAVKQMLAGRNLLFTDGDKMAADVQKFVEAAD
                     QTDFTHEATALETKVLLVRVANWRMLATRDAKGLVTFKTNVEKAQQQIAALEKAALPP
                     NLATLLASVKAGVGKYAEAFDKTGPNLLLVDELYYKAITPVIVSTIGKMDDVKASIGQ
                     AFEKTTTETEDRISNTTTTQEMVAGAAVLTGLLIAFFIARGIIGPLSGLTSGMKELAG
                     GNFGVVLPGLDRKDEVGDMAQAVETFKVKAEEKARDEAEAKVTQDLVAAKQRKADMIK
                     LADDFEGAVGEIVDTVSSASTELEASANTLTSTAERAQELTTMVAAASEEASTNVQSV
                     ASATEEMTSSVNEISRQVQDSARMAGEAVDQARKTNDRVSELSKAAARIGDVVELINT
                     IAGQTNLLALNATIEAARAGEAGRGFAVVASEVKALAEQTAKATGEIGQQISGIQAAT
                     QESVNAIKEISGTIEKLSEISSTIAAAVEEQGAATQEISRNVQQAAQGTQQVSSHITD
                     VQRGAGETGSASSQVLSAAKSLSSDSNRLKLEVGKFLNTVRAA"
     gene            2668..>3969
                     /locus_tag="VGA15_15915"
     CDS             2668..>3969
                     /locus_tag="VGA15_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9589218.1"
                     /translation="MFGAIYQIGIWSQDASRAIAGNVRTISDLNKQLSIEMLEARRNE
                     KNFQQRRNESYAKGHAELVVAINRDFDRLQILTQAGGMGDLSEKIRLAHDGFMSYARD
                     FAALVSAEVKLGLNETLGLSGSLRAAVHDIEAKLKEIDNPRSTSWMLMMRRHEKDFML
                     RRDQKYVGELKKAAAEFSKSLAAAEIPSALTAEIALKLEKYQNEFSAWADTAQQTAGY
                     DAAMMKTFRGLEPLIVEVGQGVERLYKEADAAEAATRDSVRTWMLIAFGLAVVLVCGL
                     SLLIGRSISMALSAMVRAMTRLAGGDFVVLIPSLDRRDEIGEMADAVQVFKTNMIETE
                     RLRAEQSDVEQRQLQQRKAEMHKLAAAFEGAVSEIVENVSSAATELEASASTLTSTAE
                     RAQELATAVAAASEEASTNVQSVASASEEMTSSVNEISRQVQ"
BASE COUNT          875 a         1135 c         1221 g          738 t
ORIGIN      
        1 gcggtggaag agcagggtgc ggcgacccag gaaatttccc gcaacgtgca gcaggcagct
       61 catggcaccc aacaggttag tgccaacatc accgacgtgc agcgtggcgc cagcgagacc
      121 ggctcggcct catcgcaggt cttgtcggcg gcaaaatcgc tgtccggcga cagcaatcgt
      181 ctcaagctcg aagtcggcaa attcctgaat tcggtcaggg ccgcctgacg tctcccgtat
      241 tcatacgggc actgcaatta accctgatgt agtgttacga gccgtattct atcggacgag
      301 agagcgctcg atgcgctctt cgaacgaaaa ttgtgaagtt cgccgaacgt ggcgatgttc
      361 gtctccccga tttcaactcg ccgccagcaa ggattgactc atgtcgtttc tcaaccttcg
      421 tatcacgggc cggctttacg gcggcttcgg cgcgcttctg ctgttctgcg cggcgctcgc
      481 cggtttcgcg gtatggcagt tgtcggcgat tcacacgcag gtcgggtcgc tgaccctgca
      541 atccaagaat gcgatccgcg ttggcgagat cacgactgag ctgcaggcga cccgccgctc
      601 gctgctgcgc tacgctttcg atcaggacga ggcttcgttc gcggaagccg agaagcgttt
      661 gtccaaaaca gccgaacttc ttgaggtggc cgccaagaca acgatgactg aagaacggcg
      721 cgcggcttac aaggctgccc ttccggagat caccgaattg aagaccaagc gccttgccct
      781 cggcgaggcg gtcaagcaga tgctggcggg ccgcaacctg ctgttcaccg atggcgacaa
      841 gatggcagcc gacgtgcaga aattcgtgga agccgcggat caaacggact tcacccacga
      901 ggcgaccgcc ctcgagacca aggtccttct ggtccgggtc gccaactggc gaatgctggc
      961 gacccgtgac gcgaagggtc tcgtgacctt caagaccaat gtcgaaaaag cgcagcagca
     1021 gatcgccgct ctcgagaaag ccgcgttgcc gccaaatctc gcgaccttgt tggcgtcggt
     1081 caaggcgggc gtcggcaaat atgccgaggc atttgacaag accggaccca acctgctgct
     1141 cgtggatgag ctgtactaca aggccatcac tccggtgatc gtgagcacaa tcggaaaaat
     1201 ggacgacgtc aaagcctcga tcgggcaggc tttcgagaag accaccaccg agaccgaaga
     1261 ccggatctcc aacaccacca ccactcagga gatggttgcc ggcgcagcgg tgctgaccgg
     1321 cctgctgatc gcgttcttta tcgctcgcgg cattatcggc cctctgtccg gactcacctc
     1381 cggcatgaag gaactggccg gcggcaattt cggcgtggtg cttcccggcc tcgaccgcaa
     1441 ggacgaagtc ggcgacatgg cgcaagcggt cgagaccttc aaagtcaagg ccgaggagaa
     1501 ggcccgcgac gaagcggaag ccaaggtcac gcaggatctg gtcgccgcaa agcagcgcaa
     1561 ggctgatatg atcaagctcg ccgatgactt cgaaggcgcg gtcggcgaga tcgtcgatac
     1621 cgtatcgtcg gcttcgactg aacttgaagc ctccgccaac acgctcacct caaccgccga
     1681 gcgcgcgcag gaactcacga cgatggtcgc cgcggcgtcc gaagaagcct ccaccaatgt
     1741 ccagtcggtg gcctccgcga ccgaggagat gacatcgtcg gtcaacgaga tcagccgcca
     1801 ggttcaggat tcggcgcgga tggccggcga ggccgtggat caggcgcgca agaccaacga
     1861 ccgcgtcagc gagttgtcga aagccgcagc ccgtatcggc gacgtggtcg agttgatcaa
     1921 cacgattgca ggacagacca atctgctcgc cttgaacgcc accatcgagg cggcgcgcgc
     1981 cggcgaggcc ggccgtggtt tcgcggtcgt ggcgtccgag gtcaaggcgt tggccgagca
     2041 gaccgcgaag gcgaccggcg agatcggtca gcagatcagc ggcatccagg cggcaactca
     2101 ggagtcggtg aacgcgatca aggaaatcag cggcactatt gaaaaattgt ccgagatctc
     2161 ctcgaccatc gcagcagccg tggaagagca gggtgccgcg acccaggaaa tttcccgcaa
     2221 cgtgcagcag gcagcgcagg gcacccagca ggtgagctct cacatcaccg acgtgcagcg
     2281 cggcgccggc gagaccgggt cggcctcgtc gcaggttctc tccgcggcaa agtcgctgtc
     2341 cagcgacagc aaccgtctga agctcgaagt cggcaagttc ctcaacacgg tgagggccgc
     2401 ctgacaacac tgatggtctg gcccatcagg gggccaggtc catgaccctg gtcaggttga
     2461 cgctcgcata catttcaatt ctcacctgca tcatttttag acggatctta atgactaaca
     2521 aatagtatgc gatcgcggcc gacacagttt cgttggcgtg gctctagatt tcgaaaatat
     2581 agaggtatcc cgatgcgcga gcgatttcat ctgcgcttga tccacaagat catggcaatc
     2641 gggatcgtcg gtctgttcgg gcttcttgtg ttcggcgcga tctaccagat cggcatctgg
     2701 tcgcaggatg catcgcgcgc cattgcgggc aatgtccgta cgatctctga tcttaacaaa
     2761 cagctttcga tagaaatgct ggaagcgcgt cggaacgaaa agaatttcca gcagcgccgc
     2821 aatgagtctt atgcgaaggg tcacgcggag ttagtggttg caatcaaccg tgatttcgat
     2881 cggcttcaaa tattgaccca agccggcggc atgggcgatc tctccgaaaa gattcggctc
     2941 gcgcatgatg gcttcatgag ctatgcacgc gatttcgcag cattggtgag tgccgaagtc
     3001 aaactcggcc ttaatgaaac attgggtttg tcggggtcgc tgcgcgcagc cgttcacgat
     3061 atcgaagcga aactcaagga aatcgacaat ccaagatcga ccagctggat gttgatgatg
     3121 cgccggcatg agaaggactt tatgctgcgg cgggaccaga aatacgtcgg tgaactgaaa
     3181 aaggccgcgg cagagttttc taaatctttg gctgccgccg aaattccctc ggccctgacg
     3241 gccgaaattg ccctaaagct ggagaaatat cagaacgagt tttcggcttg ggccgatacc
     3301 gctcaacaga ccgcaggcta tgacgccgcg atgatgaaga cgtttcgggg gctggagcct
     3361 ctgattgttg aagtgggaca aggcgtagag cgactttaca aggaggctga cgcggccgag
     3421 gcagccacgc gcgactcggt aagaacatgg atgctgatcg catttggact ggcggtcgtt
     3481 cttgtatgcg ggttgtcgtt gctgatcggt cgatcgatat cgatggcact ttcggcaatg
     3541 gtccgagcca tgaccaggct ggcgggcggt gattttgtag tccttatacc gagccttgac
     3601 cgcagggatg agatcggcga aatggccgat gcggtgcagg tgttcaagac caatatgatc
     3661 gaaacggagc ggctgcgcgc cgagcaatcg gatgtcgagc aacggcaatt gcagcagcgc
     3721 aaggcggaaa tgcacaagct tgccgctgca ttcgaaggcg cggtgagcga gatcgtcgaa
     3781 aacgtgtcct cggctgcgac cgagcttgaa gcttctgcca gtacgctgac gtcgactgcc
     3841 gaacgggcac aagagctcgc aaccgcggtt gcggcagcgt ccgaagaagc ctctaccaat
     3901 gtgcagtcgg tggcctccgc gagcgaggag atgacctcgt cggtcaacga gatcagccgc
     3961 caggttcag
//
LOCUS       DASWGM010000557         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1730533,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000557 DASWGM010000000
VERSION     DASWGM010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730533"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGA15_15920"
     CDS             <1..444
                     /locus_tag="VGA15_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9589219.1"
                     /translation="TPLLYVLRDDIKLNAAKFGCGLGQCGSCTVIVDGKAVLSCVTPL
                     LLLEGKQVTTLEGLGTLEAPAPIQRAFMEEQAAQCGYCIAGMMMRAQALLQRNPKPTD
                     EQIRSELEPHLCRCGTHMRILRAVHRASRLMDTADASSATQRSAP"
     gene            441..2675
                     /locus_tag="VGA15_15925"
     CDS             441..2675
                     /locus_tag="VGA15_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9589220.1"
                     /translation="MNASMILDRRRVLAGGGALIVSFSLTDAFAQDQTAPATAPAQNP
                     PGSLKGAPFLDSWIRIDADGSITAFTGKAELGQGFKTAFQQIAAEELDVPFESLKVVT
                     ADTRLTANEGYTSGSHSMQDSGTAIQNAAAQVRELLVAEAARRLDLPAENFRTENGAV
                     ISSDGRRFAYGDLVAADMLHVQAQPKSKLKDPATFKVMGQPIPRVDIPAKVTGGAAYV
                     QDMRLAGMVHARIVRPPSYGAQLTDCDTSAIEKLPGVVKVIRDGNFLAVVAAKEFLAV
                     KAMKALSAAAKWKETAGLPKQDDLLHVLTSLPSQDSTIFQQSNPSAVGQKTIEATYTR
                     PYQAHGSIGPSCAVAQLVDGAMTVWTHTQGVYPDRQGIAEMLRVPPASVRLIHVEGSG
                     CYGHNGADDAAADAALIARALPGTPVRVQWMREQEHLWEPFGPAMVTKLKASLDGNGA
                     ISDWNFEVWSNTHSMRPGGAGSMLAAQHMAQPFAVPAPKPLPLPEGGGDRNAIPIYTF
                     PNAHVVHHFIPAMPLRISAMRSLGAYHNVFSIESFMDELADAAGADPVEFRLKHLDDP
                     RGREVIEKAAQGFGWLKGQQAPQDRGFGFAFARYKNLAAYCAIATEVEVNRETGRPRL
                     VRAVAAVDSGQVVNPDGLINQIEGAIVQSMSWTLYESVTFDDTRITSIDWQTYPILRF
                     NSVPEGIEVHIINRPGKPFLGSGETGQGPAAASIANAIADATGKRLRDLPLTRKRIKD
                     AIDA"
     gene            2755..>3486
                     /locus_tag="VGA15_15930"
     CDS             2755..>3486
                     /locus_tag="VGA15_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX9589221.1"
                     /translation="MTHAIRFHQTGGPEVLVWEEVKLGKPGPGEARIRHTAVGLNFVD
                     IYNRSGLYPAELPSGLGSEGAGVVEEVGPGVSDLKPGDRVAYGNGPLGAYAEARLIPA
                     DRLLKLPDGIDDKTAAAMMLKGLTVQYLIRQTYRVKAGDTILLHAAAGGVGLILGQWA
                     KHLGATVIGTVGSDEKAKLAKAHGCAHTVVYTREDFVKRVGEITEGKKVPVVYDSVGK
                     DTFLKSLDCLAPLGVVALFGQSSGSV"
BASE COUNT          653 a         1100 c         1123 g          610 t
ORIGIN      
        1 acgccgctgc tttatgtcct gcgcgacgat atcaagctca atgccgccaa gttcggctgc
       61 gggctcggcc aatgcggttc gtgcacggtg atcgtcgacg gcaaggcggt gttgtcgtgc
      121 gtgacgccgc tgcttctgct cgagggcaag caggtgacga cgctcgaggg gctcgggacg
      181 ctggaagcgc ccgcgccgat ccagcgcgcc ttcatggaag agcaggcggc gcaatgcggc
      241 tattgtatcg ccggaatgat gatgcgtgcg caggccttgc tgcagaggaa tcccaagccc
      301 accgacgagc agatccgcag cgagctggag ccgcatttgt gccgctgcgg cacccatatg
      361 cgcatcctgc gcgcggtgca ccgcgccagc cggctgatgg acacggccga tgcgtcttcg
      421 gcgacccaga ggagcgcacc atgaatgcct ccatgattct cgatcgccgt cgcgtgctcg
      481 caggtggcgg cgcgctgatc gtcagcttct cgctcaccga cgcctttgcg caggatcaga
      541 ccgcgccggc cacggccccg gcgcaaaatc ctcccggcag cctcaagggc gcgccattcc
      601 tggattcctg gattcgcatc gatgccgacg gcagcattac ggcgttcacc ggcaaggccg
      661 agctcggtca gggtttcaag accgcgttcc agcagatcgc cgccgaagaa ctggatgtcc
      721 ccttcgaatc gctgaaggtc gtcaccgcgg atacgcgcct caccgccaac gaaggctata
      781 cctccggcag tcattcgatg caggacagcg gcactgcgat ccagaacgcc gcggcgcagg
      841 tgcgcgaact tctggtggcc gaagccgccc ggcgtctcga tctgccggct gaaaattttc
      901 gaaccgaaaa cggcgcggtg atttcgtccg atggccggcg ctttgcttac ggcgacttgg
      961 tcgcggccga catgctgcat gtgcaggcgc agccgaaatc gaagctgaag gatcccgcga
     1021 cgttcaaggt gatggggcag ccaataccgc gggtcgatat tccggccaag gtcaccggag
     1081 gtgcggccta cgtccaggac atgcggctag ccggcatggt gcacgcccgg atcgtacggc
     1141 cgccgagcta cggcgcccaa ttgacggatt gcgacacctc ggcgatcgag aagctgcccg
     1201 gcgtcgtcaa ggtcatccgc gacggcaatt ttcttgcggt cgtcgccgca aaggaatttt
     1261 tggcggtcaa ggccatgaag gcgctgtcgg ctgccgccaa atggaaggag acggctggtc
     1321 tgccgaagca ggatgatctg cttcacgtgt tgaccagcct gccgtcgcag gattcgacca
     1381 tcttccagca aagcaatccg tcggccgtcg ggcaaaaaac catcgaagcc acttataccc
     1441 ggccctatca ggcgcacgga tcgatcggcc cgtcttgcgc ggttgctcaa ctggtcgacg
     1501 gcgcgatgac agtttggacc cacacccagg gtgtctatcc cgatcgccag ggcatcgcgg
     1561 aaatgttgcg ggtgccgccg gcgagcgtgc gcttgatcca tgtcgaaggc tccggatgtt
     1621 acggccataa cggcgccgac gatgccgctg ctgacgcggc gctgatcgca cgcgctttgc
     1681 cgggcacgcc ggttcgcgtg caatggatgc gcgaacagga gcacctttgg gagccgttcg
     1741 gcccggcgat ggtgacgaag ctgaaggcgt cgctcgacgg caacggcgcg atatcggact
     1801 ggaatttcga agtctggagc aacacccact cgatgcggcc gggaggcgcg ggatcgatgc
     1861 tggcggcgca gcacatggcg caaccgtttg ccgtgccggc accaaaaccg ctgccgttac
     1921 ccgagggcgg cggcgaccgc aacgcgatcc cgatctacac cttccccaac gcgcatgtgg
     1981 tgcaccactt catcccggcc atgccgctgc ggatttcggc gatgcgatcg ctcggggcct
     2041 accacaatgt gttttcgatc gagagtttca tggacgaact ggccgatgca gccggcgccg
     2101 atcccgtcga attcaggctg aaacatctcg acgatccgcg cgggcgcgag gtgatcgaga
     2161 aggcggcgca agggtttggg tggctgaagg ggcaacaagc gccgcaggat cgcggatttg
     2221 gctttgcctt tgcccgctac aagaatctcg cggcttattg tgcgatcgcc acggaggtgg
     2281 aagtgaaccg ggagaccggg cgtccgcgtc tggtgcgcgc ggtggcggcg gtcgatagcg
     2341 ggcaggtggt caatccggac gggctgatca accagatcga aggcgcgatc gtgcaatcga
     2401 tgagctggac gctgtacgaa agcgtcacct tcgatgacac caggatcacc agcatcgact
     2461 ggcaaaccta tccgatcctg cggttcaact cggtccccga gggcatcgag gtgcatatca
     2521 tcaatcgacc cgggaaacct ttcctgggga gcggcgaaac cggacaggga ccggctgcgg
     2581 cgtccattgc caacgccatc gccgacgcca ccggcaaacg gctgcgcgat ttgcctttga
     2641 cgcgaaagcg gatcaaggat gcgatcgacg cgtgaacatt tctggacgcc cgctggcgga
     2701 ttccctccgc tgcgccattt gtttcctttg tcatcccatg tgaggagtaa ttccatgacc
     2761 cacgccatcc gctttcatca aaccggcggc cccgaagttc tggtctggga ggaagtcaaa
     2821 cttggcaagc ccggtcccgg cgaagcgcgc atccgccaca ccgccgtcgg gctgaacttt
     2881 gtcgacatct acaaccgctc aggcctttat ccggccgagt tgccgagcgg gctaggcagc
     2941 gagggcgccg gcgtggtcga agaagtcgga cccggcgtca gcgatctgaa gccgggcgac
     3001 cgcgtcgcct acggcaatgg accgcttggc gcctatgccg aagcgcggct gattccggcc
     3061 gatcgtctcc tgaaactgcc cgacgggatc gatgacaaga ccgcggccgc catgatgctc
     3121 aagggtctga cggtgcaata tctgatccgc cagacctatc gcgtcaaggc cggcgatacc
     3181 atcctgctgc acgccgcggc cggtggcgtc ggcctcatcc tcggccaatg ggcgaagcat
     3241 ctcggcgcca ccgtgatcgg caccgtcggc agcgatgaaa aagcaaaact tgccaaggcg
     3301 catggctgcg cccacaccgt ggtttacacg cgcgaggatt ttgtgaagcg ggtgggcgag
     3361 atcacggaag gcaagaaagt gcctgtcgtc tacgactccg tcggcaagga caccttcttg
     3421 aaatcgctcg actgcctggc gccgctcggc gtcgtcgcgc tgttcggcca atcctccggc
     3481 agcgtc
//
LOCUS       DASWGM010000558         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2561068,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000558 DASWGM010000000
VERSION     DASWGM010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561068"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VGA15_15935"
     CDS             complement(<1..449)
                     /locus_tag="VGA15_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX9589222.1"
                     /translation="MKFFGAPPAIMKAALEACRNLGLRSCCHHAQLAVGRMNALTTAQ
                     WGLTSSEHSYGLPEALFEEQTLQSYAADYDYNDEYLRFSSAGRTFLQAAKPGSKKWND
                     VLEGFLAAGHTFVPTFNVYDANRDLMRTRRADWHDRFTDPTLWAYFQP"
     gene            complement(453..1073)
                     /locus_tag="VGA15_15940"
     CDS             complement(453..1073)
                     /locus_tag="VGA15_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589223.1"
                     /translation="MTMQEYSGNAGEEFAEMFAAGGLPPVEPAVSRQRGRGPFKRLVL
                     RGATIIDGTGAPPWGPADVVVEGSRISVLKRVGTPGLPIEPARRPAAGDVEIDCAGKF
                     LTPGFIDCHAHIGAAFHAENGSMPTADYVYKLWLAHGVTTVRETGCINGLSWTLEQKQ
                     AAAEHRIDAPRIFAYAAFPGTNDYVKTIHTADAAREWLAAVKDRGA"
     gene            complement(1159..>1881)
                     /locus_tag="VGA15_15945"
     CDS             complement(1159..>1881)
                     /locus_tag="VGA15_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX9589224.1"
                     /translation="GNIYLNRNLIGAVVGVQPFGGHGLSGTGPKAGGPLYLYRLLSAR
                     PVPPPVAPTGSAPPVARLWVEYLASIGSQAVAACAEMIEISPVETVLDLSGPVGERNL
                     YMLKPRGSILCVAETATGLQIQIGAALASGNKAIVDKTSPAMAFENLPPGLQRQLAVT
                     TDRAAAKFDAVLLEGDAAAVRRISAELAARPGPIVAVHALSPDALASGTESYPLEWLL
                     TEQSVSANTTAAGGNASLMSIS"
BASE COUNT          331 a          600 c          603 g          347 t
ORIGIN      
        1 ggctggaagt aggcccacaa ggtgggatcg gtgaatcgat cgtgccagtc ggcgcgccgc
       61 gttcgcatca aatcgcggtt ggcgtcatag acattgaaag tcgggacgaa agtatgcccg
      121 gcggccagaa acccctcgag cacatcgttc cactttttcg accccggttt cgcggcttga
      181 aggaacgtcc gtcccgccga cgagaaacga agatattcgt cattgtagtc gtagtccgcg
      241 gcgtaggatt gaagcgtctg ttcctcgaaa agcgcctccg gcagcccgta ggaatgctcc
      301 gaactcgtca ggccccattg ggctgtcgtc agcgcgttca tgcgaccgac ggcgagctgc
      361 gcgtgatggc aacagcttcg caagccgaga ttgcggcacg cttcgagggc ggctttcatg
      421 atcgcgggcg gtgccccgaa gaacttcacc ccctaggcgc cacggtcctt gacagcggcc
      481 agccactcgc gggcggcgtc cgcggtatgg atcgtcttga cgtagtcgtt ggtgcccgga
      541 aacgcggcgt aggcaaagat cctgggggca tcgatgcgat gctcggcggc ggcctgcttt
      601 tgttcgagcg tccacgacag cccgttgatg catccggtct cgcggacggt ggtgacgcca
      661 tgcgccagcc acagcttgta aacgtaatcg gccgtcggca tcgaaccgtt ttcggcgtga
      721 aatgccgcgc cgatatgcgc gtggcagtcg ataaagccag gcgtcaggaa tttgccggcg
      781 caatcgatct cgacgtcgcc ggcggccggt cgacgggcgg gctcgatggg gagcccgggc
      841 gtaccgaccc tcttgagtac cgagatccgg ctgccttcca ccacgacgtc agcgggtccc
      901 cagggtggcg ctcccgtgcc gtcgatgatg gtggcgccac gcagcaccag gcgcttgaac
      961 gggccgcgtc cgcgttgccg cgaaaccgcc ggctcgaccg gcggaagacc tccggcggca
     1021 aacatttccg caaattcttc gccggcattg cccgagtatt cttgcatcgt cacagaccct
     1081 tgatccagtt ctcgtgggga gagatgtttt ccgaactcac ttccgtcgag aacgacggat
     1141 gtccttacgg tctcgttgtc aactgatcga catcaggctc gcgttgcctc ccgccgctgt
     1201 cgtattggcg ctgacggatt gctccgtcag cagccattca agcggatagc tttccgtgcc
     1261 cgaggcgagt gcgtccgggc tgagcgcgtg gacggcaaca atcggtcctg gacgggcggc
     1321 cagttcggcg ctgatccggc gaaccgcggc cgcgtcccct tcgagcaaaa cggcgtcgaa
     1381 cttggccgcc gcccgatccg tcgtcaccgc gagttgccgc tgcaacccgg gcggcaggtt
     1441 ttcgaaggcc atggccggtg acgttttatc gacaatcgcc ttgttgccgc tcgccaacgc
     1501 agcgccgatc tgaatctgca gaccagttgc ggtttcggcg acacagagga tcgacccgcg
     1561 cggcttcagc atatagaggt tacgctcgcc gaccgggccg gacaggtcga gcaccgtctc
     1621 caccggcgat atctcgatca tttctgcgca agctgcgacg gcttgcgaac ctatcgatgc
     1681 caggtactcg acccagagcc ttgcgaccgg cggcgctgaa ccggttggcg cgaccggcgg
     1741 cggcaccgga cgcgccgaca gcagacgata gagatagagc gggccgccgg ctttcggtcc
     1801 ggtccccgac aggccatggc ctccgaaggg ctgcacgccg accaccgcgc cgatcaaatt
     1861 gcggttcaga tagatgttgc c
//
LOCUS       DASWGM010000559         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2003481,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000559 DASWGM010000000
VERSION     DASWGM010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003481"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1862
                     /gene="phaC"
                     /locus_tag="VGA15_15950"
     CDS             57..1862
                     /gene="phaC"
                     /locus_tag="VGA15_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX9589225.1"
                     /translation="MSDVTAETQVAKKFDPEAFAMNLARAMESSGQALAAYLKPRESG
                     EITDKPPGELAEVIKTFSSVAEYWLSDRERSAELQIKIGKAYLDLWGSAVRRLAGEQA
                     EPAIAPSPRDKRFNDPEWKSNQFFDFVMQLYLLTTQWAQDLVRNAEGLDPHTRKKAEF
                     YVQQIANALAPSNFVLTNPEVLRETLASSGDNLVRGMKMLAEDIEAGGGMLRIRQSDP
                     SNLVVGVNMATTPGKVIFQNELMQLIQYQPSTENVLRTPLLIVPPWINKFYILDLRPE
                     KSFVKWCVDQGITVFVISWVNPDKRLGSKTFDDYMKEGPLAAMDAIEKATGELKVHTM
                     GYCVGGTLLASTLAWLAEKRRVRVTSATFLAAQVDFTHAGDLLVFVDEGQISALERDM
                     QAAGVLEGSKMAMAFNMLRSNDLIWSYVVSNYLKGQPPSSFDLLHWNSDATRMPAANH
                     SYYLRNCYLENRLSTGSMVLDNTLLDLSKVKVPIYNLATREDHIAPADSVLYGSQFFG
                     GPVKYVLSGSGHIAGVVNPPSSGKYQFWTNDNIKDVTLADWLKGAQEHKGSWWPDWRE
                     WLASIDAEEVPARAVGTEALPPIEDAPGSYVRVRA"
     gene            1938..>2327
                     /locus_tag="VGA15_15955"
     CDS             1938..>2327
                     /locus_tag="VGA15_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX9589226.1"
                     /translation="MTRELFWLTLTVIFTGLLWIPYIINRCQVRGLSGTMANPSRNAK
                     PHAEWATRLMFAHDNAVENLVIFAPLVLILAEIDYSTKWTVYACAVYFWSRVAHLIVY
                     TLGLPVFRTLAFTVGFLAQAVLALAIFK"
BASE COUNT          463 a          690 c          720 g          454 t
ORIGIN      
        1 gcaagcgtcg atccagcgcg gtgacgaact cgaaatgaac ctatagtgtt gcggatatga
       61 gcgacgttac ggccgaaacc caggttgcga aaaagttcga ccccgaagcc ttcgcgatga
      121 acctcgcgcg ggccatggag agcagcggcc aggcgctcgc agcctatctc aagccgcgcg
      181 agagcggcga aatcaccgat aagccgccgg gcgaactcgc cgaggtgatc aagactttca
      241 gctcggtggc ggaatattgg ctgtcggaca gagagcggtc ggcggaactg cagatcaaga
      301 tcggcaaggc ctatctcgat ctctggggtt cggcggtgcg ccggcttgcc ggcgaacagg
      361 cggagccggc gatcgcgcca tcgccgcgcg acaagcgatt caacgatccc gagtggaagt
      421 cgaatcagtt tttcgatttt gtgatgcagc tctatctgct cacgacgcaa tgggcgcagg
      481 acctggtgcg caatgccgaa gggctcgacc cgcatacccg caagaaggcc gaattctacg
      541 tccagcagat tgccaacgcg ctcgcgccat cgaatttcgt tctcaccaat ccggaagtat
      601 tgcgcgagac gctggcgtcc agcggcgaca acctggttcg cggcatgaag atgctggcgg
      661 aggatatcga ggccggaggc ggcatgctgc gcattcgtca atccgaccct tccaacctag
      721 tggtcggcgt caacatggcg actacgccgg gcaaggtgat cttccagaat gagctaatgc
      781 aactgatcca gtatcagccg tcgacggaaa acgtgctgcg cacgccgctt ctgatcgtgc
      841 cgccgtggat caacaagttc tacatcctcg acctcaggcc ggaaaaatcc ttcgtcaagt
      901 ggtgtgtcga tcagggcatc accgtgttcg tgatctcgtg ggtcaacccg gacaagcgct
      961 tgggctcgaa aaccttcgat gattacatga aggaaggccc gctggcggcg atggatgcca
     1021 tcgagaaggc caccggcgag ttgaaagtcc acaccatggg ctactgcgtc ggcggcaccc
     1081 tgctcgcctc gacgctggcc tggcttgcgg aaaagcggcg cgtgcgcgtg acgtcggcga
     1141 ctttcctggc ggcgcaggtc gatttcaccc acgccggcga ccttctggtg ttcgtcgacg
     1201 agggccagat ctcggcgctc gagcgcgaca tgcaggcagc gggcgtgctc gaaggcagca
     1261 agatggcgat ggccttcaac atgctgcgct ccaacgatct gatctggtcc tatgtggtca
     1321 gcaactacct gaagggacaa ccgccctcct cgttcgatct cttgcactgg aattcggacg
     1381 cgacgcggat gccggcggcg aaccattcgt attacctgcg caactgctat ctggaaaacc
     1441 ggctgtccac cggcagcatg gtgctggaca acaccttgct cgatctgtcg aaggtgaagg
     1501 tgccgatcta caatctggcg acgcgcgagg atcatatcgc gccggcggat tccgtgcttt
     1561 acggctcgca atttttcggc ggcccggtga agtatgtact gtcggggtcc gggcatattg
     1621 caggcgtggt caacccgcca tcgtcgggca agtaccagtt ctggaccaac gacaatatca
     1681 aggatgttac gctcgccgac tggctcaagg gcgctcagga gcacaagggc tcgtggtggc
     1741 ccgactggcg cgaatggctc gcaagcatcg acgctgagga ggtgccggcg cgcgcggtcg
     1801 gcaccgaggc gttacctccg atagaagacg cgcccggcag ctacgtccgg gtccgcgcat
     1861 agcgcaacaa agcacctttg agtataatcg gtcgtccccg ccgggacgcg attcgaaatc
     1921 gattgagggg aaagcctatg acgcgcgaat tgttctggct gacgctgacg gtcattttca
     1981 ccggattgct gtggatcccc tacatcataa atcgctgtca ggttcgtggc ctctcgggca
     2041 cgatggccaa tccatcgcgc aacgccaagc cgcatgcgga gtgggccact cgcctgatgt
     2101 tcgcgcatga caatgcggtc gagaacctcg tcattttcgc gccgctggta ctgatccttg
     2161 ccgagatcga ctattcgacg aaatggaccg tctatgcctg cgcggtgtat ttctggtccc
     2221 gcgtcgcgca tctgatcgtc tatacgctgg gtctgccggt gtttcgcacg ctggccttca
     2281 ccgtcggctt cctcgcgcag gccgtgctgg cgctggcgat tttcaag
//
LOCUS       DASWGM010000560         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2003533,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000560 DASWGM010000000
VERSION     DASWGM010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003533"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..293
                     /locus_tag="VGA15_15960"
     CDS             108..293
                     /locus_tag="VGA15_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589227.1"
                     /translation="MSASFHNADKRSHRRVMLVGLLFCAAFVAISYFARQQPENHDAL
                     LKTDRLVRTAGNPPPAN"
     gene            complement(438..1319)
                     /locus_tag="VGA15_15965"
     CDS             complement(438..1319)
                     /locus_tag="VGA15_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9589228.1"
                     /translation="MSDGLQELRVFVRAAESGSFSRAARELGLSQPSVSRIIGELEAR
                     LGVTLLLRTTRRITVTDAGALFLDRAREILAEIEDAEDAARGIDSLRGIIRLALPVIY
                     GTREIIPRLPKFLAAHPLLQVEMAVADARQDLVAEGADVAIRLGELNDSGFGARKLET
                     LEIMLVASPLYLKARGTPKKPADLASHDCIFGPGNFGRDSWSFTRNGTETSVDVRGRI
                     HTNSGPGVFASAMAGLGIAMASTVMLAAEVKAGALVPLLRGYKLEPVDVHAVFPGGPR
                     PSTKVRALVDFLVKELK"
     gene            1432..2109
                     /locus_tag="VGA15_15970"
     CDS             1432..2109
                     /locus_tag="VGA15_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX9589229.1"
                     /translation="MALQDRLDAFKADFESGRFPLKPTREVLEAMHRATAELIDSAQA
                     QRAKKAGDAAPEFTLRDPDGKPVSSRELLGRGPLVISFYRGVWCPYCNLELQALQSAL
                     PDIAARGASLIAISPQTAPNSRKSQRDNKLGFPILSDTKSEVAHAFGIRFALPDYLVE
                     LYKGFGSDLAVLNDDPAWVLPMPARYVIGTDGVIAYSEVNPDYTQRPDPSELLPVLDR
                     LKASKAA"
     gene            complement(2189..>2636)
                     /locus_tag="VGA15_15975"
     CDS             complement(2189..>2636)
                     /locus_tag="VGA15_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9589230.1"
                     /translation="NGTGGLEDRLPLLWTAGVTTGRLTKEEFVAVTSSNIARILNIYP
                     RKGRLSAGSDADIVVWDPAASKNITAKNQLSRIDYNVFEGFACTGAPVVTLSRGRIAF
                     AKGDLRAEKGDGRYIERPPFSPVHVANSTWKLQNAPQAVRRSDVTP"
BASE COUNT          522 a          830 c          790 g          494 t
ORIGIN      
        1 aaagattaga acagttttcg acaatttgcg ccggcaagca accctcataa gttaggctaa
       61 atcggaagtc agattgcgcg ccaaacccat agcgatatgg gttgtcgatg tccgcttcat
      121 ttcataacgc cgacaagcgc agccaccgcc gagtaatgct ggtgggcctg ctgttttgcg
      181 cggcctttgt cgcgatcagc tattttgcca gacagcagcc cgagaaccac gacgctctcc
      241 tgaagacgga ccggctcgtt cgcaccgccg gcaacccgcc gcctgccaac tagaacgtgg
      301 cattcactgc ctggcctggc caatcccgac acatgcttgc cggtcgatat actgggaatg
      361 ttgggcggga gattgcagcc ttggctcacg acggtgcagc atgagtccta agtcgcagtt
      421 cggatgctgc gatcggccta tttgagttct ttcaccaaaa aatcgaccag cgcgcggacc
      481 tttgtcgagg ggcgcgggcc gccgggaaaa accgcatgca cgtccaccgg ttcgagcttg
      541 tagccgcgaa gcagcgggac cagcgcgccc gcctttactt cggccgccaa catgaccgtc
      601 gatgccatcg cgataccaag acctgccatg gcgctggcga agacgcccgg gccggagttg
      661 gtgtgaatac ggccgcgcac gtcgaccgat gtttcagtac cgttgcgggt gaatgaccag
      721 ctgtcgcggc cgaaattgcc cggcccaaaa atgcaatcgt gtgaggcgag atctgccggc
      781 ttttttgggg tgccacgcgc tttcaggtag agcggcgagg cgacgagcat gatctcgagc
      841 gtctcaagct tgcgcgcgcc gaaaccggaa tcgttcaatt caccgaggcg aatggcgacg
      901 tcggcgcctt ctgcgacgag atcctgccgc gcatcggcaa ccgccatttc gacctgcaac
      961 aacgggtgcg cggcgagaaa ttttggcagg cgtggaatga tttcgcgcgt cccatagatc
     1021 acaggcagcg cgaggcggat gatgccgcgc agcgaatcga tgccgcgcgc ggcgtcttcg
     1081 gcgtcctcga tttcggcgag gatctcgcgg gcgcgatcga gaaacaatgc gccggcatcg
     1141 gtcaccgtga tgcggcgcgt cgtgcgcaga agcagcgtga cgccgagccg cgcctccaac
     1201 tcgccgatga tgcgcgacac cgatggctgc gacaggccga gttcgcgagc cgcccgcgaa
     1261 aagctgccgc tttccgccgc gcgcacaaaa acccgcaatt cctgcaatcc gtcactcatt
     1321 tgtattccaa ataaatcatg tccagatttg ccatataccc cttttccggc aaatgaacca
     1381 gatcttggag gccaacccaa tcaacggaga tttcccatgg ccccgcaaga tctggccctg
     1441 caagaccgcc tcgacgcttt caaagccgat ttcgaatccg gccggtttcc cctcaagccg
     1501 acccgggagg tgctggaggc gatgcaccgc gctaccgccg aattgatcga cagcgcacag
     1561 gcgcagcgcg cgaaaaaagc cggcgacgcg gcgcccgaat tcacacttag agatcccgac
     1621 ggcaagccgg tgtcgtcgcg cgaactgctc ggcagaggcc cgctggtcat ttccttctat
     1681 cgcggcgtct ggtgtccgta ttgcaatctc gaattgcagg cgttgcaatc ggcacttccc
     1741 gacatcgccg cgagaggagc cagcctcatt gcgatctcgc cgcagacggc tccgaacagc
     1801 cgcaaatcgc agcgcgacaa caaactcggc tttccgattc tgagcgatac caaaagcgaa
     1861 gtggcccacg cgttcggcat ccgcttcgcg ctgccggatt acctggtcga gctctacaag
     1921 ggcttcggga gtgatctggc tgttctcaac gacgatccgg cctgggtgtt gccgatgccg
     1981 gcgcgctatg tgatcggcac cgacggcgtg atcgcttatt cggaagtcaa tcccgattac
     2041 acccagcgac ccgacccgtc ggagctgctg cccgtgctgg atcgtctgaa ggccagcaag
     2101 gcagcctgac ctcaacaagc cggcgcggtc gacgcgccgg tccccccagc aatctggagc
     2161 cccgtttcga ttccatcgaa acgggactct acggcgtgac gtcggaacgc cgcaccgctt
     2221 gcggggcgtt ctgcaatttc caggtcgagt tggcgacatg gaccggcgag aacggcggcc
     2281 gctcgatgta acgtccgtcg cccttctcgg cgcgaaggtc gccttttgcg aaagcgatcc
     2341 tgccgcgcga cagcgtgaca accggcgcgc cggtgcaggc aaaaccctcg aacacattgt
     2401 aatcgatgcg gctcaattgg tttttggcgg tgatgttttt gctcgccgcc ggatcccaca
     2461 ccacgatgtc ggcgtccgag ccggcggaaa gccgcccttt tctcggatag atattgagga
     2521 tgcgggcgat gttcgaagac gtcaccgcga cgaattcttc tttggtcagc cggccagtgg
     2581 tgacgccggc ggtccagagc agtggcaggc ggtcttcgag gccgccggtg ccgttc
//
LOCUS       DASWGM010000561        10960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_593471,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000561 DASWGM010000000
VERSION     DASWGM010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10960
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593471"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VGA15_15980"
     CDS             complement(<1..756)
                     /locus_tag="VGA15_15980"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9589231.1"
                     /translation="MKNDPVVAIAGVTGAVGAEFIATMDKRAFRVGKLKALASARSVG
                     KTIDFRGDRIAIEELNEASFEGVDIALFSAGGSIARKFAPIAVRAGAVVIDNSSVFRM
                     DPNVPLVIPEINANRIAEHKGIIANPNCSAITALVPLWPIHKVNRIKRVILSTYQAAS
                     GAGAAAMRELVDSTHACLDGQTYQPKVLPHSYAFNLFNHDSTVDAATGYNGEETKVMQ
                     ETRKIFEDDGIAVGVTCVRVPVLRAHSQAITFEC"
     gene            1012..2145
                     /gene="tgt"
                     /locus_tag="VGA15_15985"
     CDS             1012..2145
                     /gene="tgt"
                     /locus_tag="VGA15_15985"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX9589232.1"
                     /translation="MSLPNHFDLLATDGPARAGRLTTPHGVVRTPAFMPIGTAGAMKG
                     VHWRDVRDAGTDIVLGNTYHLMLRPGAERIAALGGLQSFTGWRGPMLTDSGGFQVMSL
                     AQLRKVSESAVTFRSHIDGAKVELSPERAIEVQLLLGSDIAMQLDECVRLPAERGDVE
                     RAMQLSLRWAERCKRAFETAALGYMLFGIAQGGDVPELRRASARGLVDIGFHGYAIGG
                     LAVGEPQAVMLAMIEEVAPILPQDRPRYLMGVGTPEDMLEAVARGIDMFDCVMPTRNG
                     RHGVAFTRFGQVNLRNARHADDPRPLDEQSAWPSTRSCARAYLHHLVKSGETLGAMLL
                     SEINVAYYQHLMRGIGEAISKGTFESFQERTRADWAKGDIAPR"
     gene            complement(2306..2521)
                     /locus_tag="VGA15_15990"
     CDS             complement(2306..2521)
                     /locus_tag="VGA15_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589233.1"
                     /translation="MSAVATILSTETRHPDPRGCDLPTCPVCADSMVAAEASAYLSDN
                     VISYLWTCDTCGYGFVTKHSVKRFACN"
     gene            2742..3719
                     /gene="cysK"
                     /locus_tag="VGA15_15995"
     CDS             2742..3719
                     /gene="cysK"
                     /locus_tag="VGA15_15995"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX9589234.1"
                     /translation="MNASPAPSAVHRPGRGRVFDSIVDAVGDTPVVRLQTLPQANGVH
                     ATILAKLEYFNPAASVKDRIGAAMIIAMEKAGLINADTVLIEPTSGNTGIALAFVAAS
                     RGYRLKLVMPESMSIERRKMLAFLGAEIVLTPAAQGMKGSIATAEELVRTTPNAVMPQ
                     QFKNLANPEIHRRTTAEEIWNDTGGNIDVFVAGVGTGGTITGVGQVLKPRKPSLRIVA
                     VEPEESPVLSGGQHSPHKIQGIGAGFIPEILDRSVIDEIVKINSSTAIETTRALARNE
                     GIPGGISSGAAIAAALQIGKRPESAGKTILAIVPSFSERYLSTALFEGI"
     gene            3722..3961
                     /locus_tag="VGA15_16000"
     CDS             3722..3961
                     /locus_tag="VGA15_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEX9589235.1"
                     /translation="MADQPRRPRTLNDARTEAEAAFKRTTTKVPEAPPKSTALPGVKE
                     LVSLRIDQDVLEHFQAGGPGWQDRINEALRKAAGK"
     gene            3976..4356
                     /locus_tag="VGA15_16005"
     CDS             3976..4356
                     /locus_tag="VGA15_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589236.1"
                     /translation="MHSRTGGFRLVLFVGLVSGLVLGLGPLMSGLLSTPASAETVPFN
                     CTILPGAEMASITMTNSLASDASCIVTCKFSTTKYDNNPQITCAKPVPAGKEVEMCRL
                     PDGGDRLVKLTEGHAECTRYQRRD"
     gene            complement(4391..5821)
                     /locus_tag="VGA15_16010"
     CDS             complement(4391..5821)
                     /locus_tag="VGA15_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9589237.1"
                     /translation="MRYLTLIVCLICMIFTAHAAKADRRVAFVVGNGAYKNVAQLPNP
                     PVDAKAMAAVLRNVGFEVVEGTNLTRDTMTERLLEFGKKAQGADVAVFFYAGHGIAIS
                     GTNYLLPVDADIKSEMDVKLGAAINIDLTLDQTMSDAKVKLVFLDACRDNPFAAKIKS
                     NSATRSVSVQSGLAEMKSGEGTLIAFATGPGQTALDGKEGTNSPFTRALIAHIATPGV
                     EIQQAMTEVRAQVNEETNKGQLPWGHTNLIGAVYLNPSAAPAASAAAPAVTGSTAAAS
                     TSSGSDVELEFWRSVKESNKPEELNAYLTNFPNGKFKSLALARIASLESGPSTATRTL
                     TAGVDPATFTEEASQTTEDQIGLDKGRRRDVQRRLTGLGFDTKQTGIFDDPTRAMITR
                     WQAARGYPKSGFLNKPQHKALLTEIVTGAPTAAREAPVEKPARPARASAGNAAAPPPP
                     QRGPDPGGAAFVGGLMGGVVGGMLRR"
     gene            complement(6007..6885)
                     /locus_tag="VGA15_16015"
     CDS             complement(6007..6885)
                     /locus_tag="VGA15_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9589238.1"
                     /translation="MDRTPSKPRAALWMAGWLALMLIMAIAGRETTRELNVFQIMEVR
                     SILGFFMLYPLIRLHGGFSAMKTSRPLQHVGRNLIHYAAQLGWFFALTLIPLGQVVSI
                     EFTMPIWTAILAASFLGERITAGKIAAIVLGVVGVLIIVRPAAGEINPGQLIALGAAV
                     GFGISIAMMKSLTRTENTLTIIFWMLVIQSAAGFFPALYVWIWPSAYAFGWIVVIAFC
                     GTFCHYCMARAMLYADATVVLPMDFLRVPLTATAGWLIYSERLDMFTVLGAALILAGN
                     LLNLRAADPVPARIGT"
     gene            7049..7162
                     /locus_tag="VGA15_16020"
                     /pseudo
     CDS             7049..7162
                     /locus_tag="VGA15_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
     gene            7174..7389
                     /locus_tag="VGA15_16025"
     CDS             7174..7389
                     /locus_tag="VGA15_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589239.1"
                     /translation="MTNRSTSADFVTAFATGWPEDEPDIMVLSLTTHKGVQDFALTPE
                     QALLIAKTMKQTAARLTVPKGVSAKRS"
     gene            complement(7508..8071)
                     /locus_tag="VGA15_16030"
     CDS             complement(7508..8071)
                     /locus_tag="VGA15_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589240.1"
                     /translation="MARRQACRAAVDGKPRGNRPGRTITALAGLRWLLVAAIALLAAS
                     VSGTASYAQSGPFTGMAGNWSGGGTVTLDDGSTERIRCRASYAVGAGGNGLNQNLLCA
                     SDSYKFDLRSNVMAEGGSLSGTWSESSRGVNGTLEGRGGNGNFQVLASAAGFNANISL
                     TTRGNRQSLVIRSESQFRGASISLSRH"
     gene            complement(8016..8378)
                     /locus_tag="VGA15_16035"
     CDS             complement(8016..8378)
                     /locus_tag="VGA15_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589241.1"
                     /translation="MRHNRICKIFCAFIVTSAAAALSAIPAAAQTPYDGLWNVTIVTK
                     AGSCEPSTRSTLTVADGKVSAAGADVSGSIGREGLVRVSIGGAYANGQLSGNTGSGKW
                     NGASAGVPCSGRWEASRQ"
     gene            complement(8583..9173)
                     /locus_tag="VGA15_16040"
     CDS             complement(8583..9173)
                     /locus_tag="VGA15_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEX9589242.1"
                     /translation="MRITLVGSRHFGVTTFNMLRQHGVEIAGVVVADAEDRLAAAARA
                     AGIGVTVQADPRLVVAREIADRTDLIVTAHSHARIAREALAAAKLGGIGYHPSLLPRH
                     RGIAAVEWTIREGDPIAGGTVYHLADRMDAGAIAAQEWVFVKKGETARELWERALAPL
                     GQKLLGEVIDYAKIHNALPAKAQDEQFATNAPKLSK"
     gene            9516..9782
                     /locus_tag="VGA15_16045"
     CDS             9516..9782
                     /locus_tag="VGA15_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589243.1"
                     /translation="MLSLNSQRQSNHGIHWPAIIATLLVQILVVLALSVAVAFYLEWS
                     SDANQAEFARATRPSSVSDSNHLPEVSIPIRTVRGGAGCYPKDR"
     gene            complement(9814..10053)
                     /locus_tag="VGA15_16050"
     CDS             complement(9814..10053)
                     /locus_tag="VGA15_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9589244.1"
                     /translation="MYERRAIPRQRIFKAGTIEFDGTGVDCIVRNISAIGAGLEVASP
                     EGIPHEITLSIRSGEVRQHAYIVWRKEKRIGVAFG"
     gene            complement(10333..10920)
                     /locus_tag="VGA15_16055"
     CDS             complement(10333..10920)
                     /locus_tag="VGA15_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589245.1"
                     /translation="MSTSALLACLALLYGIANADPAVFTVAGADPDTAVSATVAAGLK
                     SVSVSLSGLGDRVGPQTPPGGTSHGNAGSAGGTTVATAEPLVADIQPFNVRVDPNANQ
                     SIGFDYFISVIDGAGSFTPNAADVAGLSLLTSVSFTFGAELYAVDATPVAEDPERSSR
                     EMMILGFVGVGLMPYLVARALLPRPALSAFTVQSK"
BASE COUNT         2139 a         3287 c         3486 g         2048 t
ORIGIN      
        1 gcattcgaag gtgatggcct ggctatgcgc gcgcaacacc ggcacgcgca cgcaggtcac
       61 gccgaccgca atcccgtcgt cttcgaagat cttccgggtc tcctgcatga ctttggtttc
      121 ttcaccgttg tagccggtcg ccgcatcgac cgtgctgtcg tggttgaaca ggttgaaggc
      181 gtaggagtgc ggcagcacct ttggttgata ggtttggccg tccagacagg cgtgggtcga
      241 gtccaccagc tcccgcatcg ccgccgcgcc cgcgccactg gccgcctgat aggtcgacag
      301 gatcacgcgc ttgatccggt tgaccttgtg aatcggccat agcggcacca gcgcggtaat
      361 cgccgagcag ttcggattgg cgatgatgcc cttgtgctcg gcgatgcggt tggcattgat
      421 ttccgggatc accagcggca cgttcggatc catgcggaag acggacgaat tgtcgatcac
      481 gacggcgccg gccctaaccg cgatcggcgc gaacttcctg gcaatgctcc cacccgccga
      541 gaacagcgcg atatcgacgc cttcgaatga ggcctcattg agctcttcga tcgcgatcct
      601 gtcgccgcgg aaatcgatcg tcttgcccac cgagcgcgcg ctcgccagcg ctttgagctt
      661 gccgacgcga aatgcgcgct tgtccatggt ggcgatgaat tcggcgccga cggcgccggt
      721 tacgccggca atggcgacaa cgggatcgtt cttcactttg gtctccctca ggcaataaaa
      781 aagccccaga ccttttcagg cggggctccg gtccagatga tgcgatcgtc caactacgcg
      841 cgcacgtctc ccgaagcccg acagggcttt ccggttttgg tggtgcgttt ggtgcgcgtc
      901 atcatggcgc gggaacatat gggggcctgg accggcgtca acggcttttg gagcgaaaat
      961 cggcttcaac gaggttgaac aggacgccgt tgttacgcaa taagctgctg catgagcctt
     1021 cccaatcact ttgatctcct cgcgaccgac gggccagcgc gcgccggtcg gctgacaaca
     1081 ccgcacggcg tggtgcgcac gccggccttc atgccgatcg gcaccgccgg cgcgatgaag
     1141 ggcgtgcatt ggcgcgatgt ccgcgatgcc ggcaccgata tcgtgctcgg caatacctat
     1201 catttgatgc tgcggccggg cgccgagcgc attgcagcac ttgggggctt gcagagcttt
     1261 accggctggc ggggcccgat gctgaccgat tccggcggct ttcaggtgat gtcgctggcg
     1321 cagttgcgca aggtgagcga aagcgcggtg acctttcgct cgcatattga tggcgccaag
     1381 gtcgagctgt cgcctgaacg cgcgatcgag gtacagcttt tgctcggctc cgatatcgcc
     1441 atgcagctcg acgaatgcgt gcggctgccg gcggagcgcg gcgatgtcga gcgggcgatg
     1501 caactctcgc tgcgctgggc ggagcgctgt aagcgcgcgt tcgagaccgc ggccctcgga
     1561 tacatgctgt tcgggatcgc gcagggtggc gacgtgccgg aactgcgccg ggcaagcgcg
     1621 cggggcctgg tcgacatcgg ctttcacggc tacgccatcg gaggcctcgc ggtcggcgaa
     1681 ccgcaggcgg tgatgcttgc gatgatcgag gaggtcgcgc cgatcctgcc gcaggatcgc
     1741 ccgagatacc tgatgggcgt cggcacgccg gaagacatgc ttgaggcggt ggcgcgcggc
     1801 atcgatatgt tcgattgcgt aatgccgacg cgcaacgggc gccacggcgt ggcgttcacg
     1861 cggttcggtc aggtcaattt gcgcaacgcc cgccacgccg acgatccacg gccgctcgac
     1921 gagcaaagcg cctggccgtc gacccgcagc tgcgcacgcg cgtacctgca tcacctggtc
     1981 aagtcgggcg agacgctcgg ggcgatgctg ctgtcggaaa tcaatgtcgc gtattaccag
     2041 cacctgatgc gcggcatcgg tgaggctatt tcgaagggaa cgttcgaaag ctttcaggaa
     2101 cgcacgcgcg ccgactgggc gaagggcgac atcgcgccgc gctaaagccc ttttccgttt
     2161 cgatagaatc caaaccgggc tctagatttt tgtttgacgc gttttcttca cgcaaaccgg
     2221 tttccacccc ggatcaagtc cggggcgggc tgtcgcttga aggggtaggc tttcgcttga
     2281 aaacgctccg gacgccgtcg caggatcagt tgcaggcaaa ccgcttgacc gaatgcttgg
     2341 ttacgaaacc gtaaccgcag gtatcgcagg tccagagata gctgatgacg ttgtcggaca
     2401 gataagccga cgcttccgca gccaccatgg aatccgcgca aaccgggcag gtcggcaaat
     2461 cgcatccgcg cgggtcagga tgacgtgtct cggtcgacag aattgtagcg actgctgaca
     2521 tcgtgacctc ccttgcgaat tgaatgtgag gctaacatac aacggtacgt ttgacgatgt
     2581 agcaacacaa ttgcgttggt tttacgttat ttggaggctt ttaaattttg caacgcagcg
     2641 aggcctgctc cgcgggattc tggacttgca caccggcatc aggtgtccct aattgagaat
     2701 agccgctaaa tacgcataca ttgcttaaca ggagtttcgt tatgaatgct tcgccagctc
     2761 cgagtgcagt gcaccgtcca ggccgcggcc gggttttcga cagcatcgtc gatgcggtcg
     2821 gcgacacgcc ggtcgtgcgt ctgcaaacat tgccgcaggc gaacggcgtg catgcgacca
     2881 ttctcgccaa gctggaatat ttcaatccgg cggcgagtgt gaaggaccgc atcggggctg
     2941 cgatgatcat cgcgatggag aaggccggac tcatcaacgc cgatactgtg ctgatcgagc
     3001 cgacctcggg gaataccggc atcgcgctgg cattcgtcgc cgcctcgcgc ggctatcggc
     3061 tcaagctcgt gatgccggag tcgatgtcga tcgagcggcg caagatgctg gcgtttctcg
     3121 gcgctgaaat cgttctgacg ccggcagccc agggcatgaa gggctcgatc gcgacagcgg
     3181 aagagctggt gcgcacgacg cccaacgccg tgatgccgca gcagttcaag aacctcgcca
     3241 atccggaaat tcaccgccgc accacggcgg aggaaatctg gaacgacacc ggcggcaaca
     3301 tcgatgtctt cgtggccggg gtcggcaccg gcggcaccat caccggcgtc gggcaggtgt
     3361 tgaagccacg caagccgtcg ctgcggatcg tcgccgtgga gcccgaggaa agtccggtac
     3421 tctccggcgg gcaacattca ccgcacaaga tccagggcat cggcgccggc ttcattccgg
     3481 agatcctgga ccgttcggtg atcgacgaaa tcgtcaagat caacagctcg accgcgatcg
     3541 aaaccacgcg cgcgctggcg cgcaacgagg gtattcccgg cggcatttcc tcaggtgcgg
     3601 cgatcgccgc ggcacttcaa atcggcaaac gcccggaaag cgccggcaag accattcttg
     3661 cgatcgtgcc gtcgttctcc gagcgttatc tgtcgacggc gttgttcgaa ggaatctaga
     3721 cgtggcagat caaccaagac gaccgagaac cctgaacgac gcgcgcaccg aagccgaagc
     3781 ggcattcaag cggacgacaa ccaaggtgcc ggaggcgccg cccaaaagca ctgcccttcc
     3841 aggtgtaaaa gagctggtct cgctgcggat cgatcaggat gtgctcgaac acttccaggc
     3901 gggcgggcca ggctggcagg atcggatcaa tgaggcctta agaaaagcag cgggcaaata
     3961 gtcgcggcgg tatcgatgca ttcgcgcacc ggcggcttcc ggctcgttct attcgtcggt
     4021 cttgtctccg gccttgtcct cggtcttggc ccgctgatgt ccggccttct ttcgacgccg
     4081 gcgtcggcgg agacggtgcc gttcaactgc acgattttgc cgggagcgga gatggccagc
     4141 atcacgatga cgaattcgct ggccagcgac gcgtcctgta tcgtgacctg caaattctcg
     4201 acgaccaaat acgataacaa tccgcagatc acttgcgcca aaccggtccc ggccggcaag
     4261 gaagtggaga tgtgccggct gcccgacggt ggcgacagac tggtgaaact caccgagggc
     4321 cacgccgagt gcaccagata ccagaggcgg gattgaaaaa aagcccggga tgagccgggc
     4381 tttttgacga tcagcgccga agcatgccgc ctaccacgcc acccatcagg ccgccgacaa
     4441 aggcagcacc accggggtcc ggaccacgct gagggggagg gggagcagcg gcattgccgg
     4501 cgctggcacg tgcaggacgg gccggctttt ctaccggcgc ctcgcgggcg gcggttgggg
     4561 cgcccgtgac gatttccgtc agcaaagcct tgtgctgcgg cttgttgagg aagccgctct
     4621 tgggatagcc gcgtgcggct tgccagcgcg taatcatcgc gcgcgtcgga tcatcgaaaa
     4681 tgccggtttg cttggtatcg aaaccaaggc cggtcagccg gcgctgtacg tcgcgccgtc
     4741 ggcccttgtc gagaccgatc tggtcttcgg tggtttggct tgcctcctcg gtgaatgttg
     4801 cggggtcgac gccggcggtc agggtacggg tcgcggtcga cgggccgctt tccagagagg
     4861 cgatccgggc cagcgccagc gacttgaact taccgttcgg gaagttggtc aggtaagcat
     4921 tgagttcttc cggcttgttc gattctttca ccgagcgcca aaattccagt tcgacgtcgg
     4981 aaccgctgga cgtggaagca gcggcagtgc tgccggtaac ggccggcgca gccgcgctcg
     5041 ccgccggcgc cgcgctcgga ttcagataga ccgcgccgat caggttggtg tgtccccagg
     5101 gcaattgacc cttgttggtc tcctcgttga cctgggcgcg gacctcggtc atcgcctgct
     5161 ggatctcgac gcccggcgtc gcgatgtggg cgatcagggc gcgggtaaac ggactgttgg
     5221 tgccttcctt gccatccagc gcggtctgcc cggggccggt cgcgaaggcg atcagggtgc
     5281 cttcgccgga tttcatttcc gcaaggccag actgcaccga cacgctgcgg gtcgcggaat
     5341 tcgacttgat cttggcggca aagggattgt cacggcaggc gtcgaggaac accagcttga
     5401 ccttggcatc gctcatggtc tggtcgagcg tgaggtcgat attgatcgcg gcgccgagct
     5461 tgacgtccat ttccgatttg atgtctgcgt cgaccggcaa caaatagttg gtgccgctga
     5521 tggcgatgcc gtgcccggcg tagaaaaata ccgcgacgtc ggcgccttgc gccttcttgc
     5581 cgaattcgag cagccgttcg gtcatggtat cgcgtgtcag attggtgcct tcgacgactt
     5641 cgaagcccac attgcgaagc accgccgcca tcgccttggc gtcgaccgga gggttgggaa
     5701 gctgggcgac attcttgtag gcgccgttac cgacgacaaa ggcgacgcgc cggtcggcct
     5761 tggcggcgtg cgcggtgaag atcatgcaaa tcaggcaaac gatgagggtg aggtaacgca
     5821 tctgaaatcc ccaggctgag tcgagagcaa tttcggcggc aacgcattgt cgggagccta
     5881 cgaaaatacc gcagttcgcg ccacgaaaat ttaagctgtt gcccaaatct tggcagccgc
     5941 catcgccgcg cgtattgcgt gacctagatc acgattcgcg gccgcgaccc ggagatcgcg
     6001 gaccgatcag gtgccgatgc gcgcagggac cggatcggcc gctctcagat tcaaaagatt
     6061 gccggcaagg atcagcgccg cgccgagcac cgtgaacatg tccagccgct ccgaatagat
     6121 cagccagccg gcggtagccg tcagcggaac ccgcaggaaa tccatcggca gcaccacggt
     6181 tgcgtccgca tacaacatcg cgcgcgccat gcagtagtgg cagaaggtgc cgcagaacgc
     6241 gatcacgacg atccagccga aggcatacgc cgaaggccag atccagacat agagcgcggg
     6301 aaaaaatccg gccgccgact ggatcaccag catccagaaa atgatggtca gggtattttc
     6361 ggtgcgggtc agggacttca tcatggcgat ggaaatgccg aatccgacgg cggcacccag
     6421 tgcgatcaat tgaccgggat tgatttcgcc ggcggccgga cggacgatga tgaggacgcc
     6481 gaccacaccg agcacgatgg ccgcgatctt gccggcggtg atgcgctcac caagaaaact
     6541 cgcggcaagg atcgcggtcc agatcggcat ggtgaattcg atcgatacca cctggccgag
     6601 cgggatcagc gtcagcgcga agaaccagcc gagctgcgcg gcgtagtgaa tgagattgcg
     6661 cccgacatgc tgcagcggcc gcgacgtctt catcgccgaa aaccctccgt ggaggcggat
     6721 cagcggatac agcatgaaga agccgaggat cgatcgcacc tccatgatct gaaacacgtt
     6781 cagttcgcgc gtcgtctcgc ggccggcgat cgccatgatc agcatcaggg caagccagcc
     6841 tgccatccac agcgcggccc tgggtttgga aggtgttcgg tccatcagcg ggggcgccag
     6901 gcatgccgcg caagcgccgg cgcggctggg tggggggagg gcaggccggt atcggcgacc
     6961 tcatgcaaat ttgcaacgtg caaatgtgcc aaggcagcgc tgcgcgcatc gcaatttcgg
     7021 tgtgggagcg ccggggcgat cggtgctaag ggatcggctg tggcgaaatt tcccgccctc
     7081 gacgctgctc tacatcgaag gtctcgtcga tatcggagcg aagctcgaga ttgaagttac
     7141 cgcgttcgta acgacgacat aggaagtgta gagatgacca accgatccac atccgcggat
     7201 tttgtgacgg ccttcgccac cggctggccc gaggacgaac cggatatcat ggtgctttcg
     7261 ctgaccacgc ataaaggcgt tcaggatttt gcgctcaccc cggaacaggc gctgttgatc
     7321 gcaaagacca tgaaacagac cgccgcccgg ctgaccgtgc caaaaggcgt tagcgccaaa
     7381 aggagttagc cgacgcggca aaaccggctg gagcctggct ctgctctttt gttttgacgc
     7441 gttttcttga cgcgaaccgg tttccacccc ggatcaagtc cggggcgggc tttcgctcga
     7501 aaacgctcta atggcgcgac agtgaaatcg aggcgcccct gaattggctc tcggacctga
     7561 tcaccaagga ctgcctgttg ccgcgcgtgg tcaaggaaat gttggcgttg aacccggccg
     7621 cgctcgccag cacctgaaaa ttgccgttgc cgccgcgtcc ctcgagggtt ccgttgacgc
     7681 cgcggctgga ttcgctccag gtcccggaaa gcgatccgcc ctccgccatc acattgcttc
     7741 tgaggtcgaa tttgtagctg tcgctggcgc agagcaggtt ctggttgagg ccgttgccgc
     7801 cagcacccac ggcataactg gcgcgacatc ggatgcgctc ggtcgatccg tcatcgagcg
     7861 tgacggtgcc tccgccggac cagttgccgg ccatgccggt aaatggcccc gactgggcgt
     7921 agcttgcggt gccggatacc gacgccgcca acaatgcgat ggctgcaacc agcagccacc
     7981 gtaggcccgc aagggctgtt attgttcggc ccggtctatt gccgcgaggc ttcccatcga
     8041 ccgctgcacg gcacgcctgc cgacgcgcca ttccattttc ctgatcccgt attgccgctg
     8101 agttgcccgt ttgcatatgc accaccgatc gaaaccctca cgagtccctc ccgtccgatg
     8161 ctgccagaga cgtcagcgcc ggcggccgag accttgccgt ccgcgaccgt aagtgttgag
     8221 cgcgtcgagg gttcgcaaga acctgccttg gtcacgatcg tgacattcca gagcccgtca
     8281 tagggtgtct gggccgcggc gggaatggcg ctaagggcag cggcggcaga ggtgacaatg
     8341 aaagcacaaa atatcttaca aatgcgattg tggcgcatgg gtcaaatccc cgatgtgttt
     8401 ggtgatgcgg ccttagtcta ggcacaatgt gtctaaaatt tgctgcgctg cgtcaacgcg
     8461 aaaagttccc tacagatcaa agatttgaag ttaaagctgg ttcccgctcc ggaacggaaa
     8521 tttgttccgc gatggcccgt gggacgggat ttgaacgccc gattcgcaaa ataggtatca
     8581 tactacttcg aaagcttggg cgcgttggtg gcaaactgct catcctgggc ttttgccggc
     8641 agggcgttgt ggattttggc gtagtcgatg acttccccca aaagcttctg gccgagcggc
     8701 gccagcgcgc gctcccacag ctcccgggcg gtctcgccct tcttgacgaa aacccactcc
     8761 tgcgcggcaa tggcgccggc atccatgcgg tcggcgagat ggtatacggt gccgccggcg
     8821 atcggatcgc cttcccggat cgtccattcc accgccgcga tcccccgatg cctcggcagc
     8881 agcgagggat gatagccgat gcccccgagc tttgccgcgg ccagcgcctc cctggcgatg
     8941 cgcgcgtggc tgtgcgcggt cacgatcaga tcggtccgat cggcgatttc gcgggcgacc
     9001 acgagcctgg ggtcggcctg cacggtgacg ccgatgccgg cggcacgggc cgcggcggcg
     9061 agccggtctt cggcgtcggc cacgacgacc ccagcgattt cgacgccgtg ctggcgcagc
     9121 atattgaatg tcgtcacgcc gaaatggcgg gagccgacga gagtaatccg catggtggtt
     9181 tatccgtctt gagcgtaagg catctgggcc gtcgataaca cgccgcaggc agctgtcacg
     9241 ccgcccgcag cggaggggta gcgggttatc aacaagaccg gggaaaatcg caaggcacgg
     9301 ggcacggcga ggccagcgcg cacacgacct aaaacaaaaa agccgccaag cgaaatatca
     9361 tcgcctgacg gcttcgcagt ggtggtcccg gactcggggg agatatccgg tacctgcgtg
     9421 atagcgcgtt tcgtcgcatc cggtagtgaa ccagttcaca tttgggcgtc tcgaattgat
     9481 ctatgagggt gcgaacccgg attcgggagg catcaatgct atcgctcaat agccaacgtc
     9541 aaagcaacca cggcatccat tggccggcaa tcattgcgac cctgctggtc cagattctgg
     9601 ttgtgctggc gctctcggtc gcggttgctt tctatctgga atggtcatcg gacgccaatc
     9661 aggccgaatt cgcccgcgcg accagaccat catcggtgtc cgattcaaat catcttccgg
     9721 aagtatcgat tccgattcgg acggtgcgag gcggagcggg atgctatccg aaggatcgat
     9781 gaaccagccc gtcaacaaac ggcgggcttt tcgctaaccg aacgcgacgc cgatccgctt
     9841 ttccttgcgc cacacgatgt aggcatgctg acgaacctcg ccgctgcgga tgctcagcgt
     9901 gatctcgtgg ggaatgcctt ccgggctcgc aacctcaagg ccggcgccga tggcggagat
     9961 gttgcgaacg atgcaatcga ctcccgtgcc gtcgaactcg atggttccgg ccttgaatat
    10021 gcgttgccgc ggaattgcac gtcgctcata cattccattg atttgcgcca aaaagtctaa
    10081 tagtcgggtg aatggcggca gcgaaaccac ggcgcatccg aaaacgattt ggtaaccgac
    10141 gccaatttcg cgcgggttcc agcaccccgg cggtccaatg acatggcgag cacgatccct
    10201 gtccggtagc ggacaaagcc ctgttgcttg atgcgatgaa gagcctcgtc tttcaagaag
    10261 gcgccttgcc ggggccgggg cagcaaccgg tcaggtcagg ctgaactgca cctgcccgga
    10321 gcgcgcgccc agctactttg actgaacggt gaaagcggac aaagcgggcc gcggaagcag
    10381 cgctcgggcg acgagatagg gcatgaggcc gacgccgaca aaacccaaga tcatcatttc
    10441 ccggctggag cgctcgggat cttccgcaac gggagttgcg tccaccgcgt agagttcggc
    10501 gccgaacgtg aacgagacgc tggtgaggag cgacaggcca gcgacgtcag ccgcgtttgg
    10561 cgtgaaactg cctgcgccat cgatgacgga aataaagtaa tcaaagccga tactttggtt
    10621 ggcgttagga tcgaccctca cattgaacgg ttgaatgtcc gccaccaatg gttcggcggt
    10681 agccaccgtc gttccgcccg cgctgccagc gttcccatgc gacgtgccgc cgggtggcgt
    10741 ctgcggaccg acgcgatcgc cgaggcctga cagggaaact gaaacgctct tcagaccggc
    10801 tgcgaccgtc gctgacaccg cagtgtcagg gtcggcgccg gcaacggtga agacggcagg
    10861 atctgcgttg gcgataccgt atagcaacgc caggcacgca agtaacgcac tggtactcat
    10921 atgaatttaa acgcctaaaa ggtattcaag taaatattag
//
LOCUS       DASWGM010000562         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_736422,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000562 DASWGM010000000
VERSION     DASWGM010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736422"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VGA15_16060"
     CDS             <1..1039
                     /locus_tag="VGA15_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX9589246.1"
                     /translation="KANIRIVVGDYEDRAIRPYALGKFRDLLVATLHHPAMLRYLDNA
                     DNAAGHLNENYAREIMELHTMGVGSGYAQSDVEALARILTGVGIDLKPEDPKLKPELQ
                     SQLVREGAFEFNPARHDYGDKTFLGHAIKGRGLAEVDEAIDILVRHPATATHLCRQIA
                     TYFVSDNPPEPLVQRMAQTFKATDGDIAAVLAAMVHAPEFTASLKPGARFKDPVQYVF
                     SAVRLAYDDKVILNTSSIQGWLNRLSEGLFNHDTPDGYSMTSASWNGPGQMMLRFEIA
                     RQIGSGSSGLFKPDVPNAVDQPAFPLIQNSLYFNGLRQTLGSPTLAALDQAVSPQDWN
                     TLFLSSPEFMR"
     gene            1143..>1823
                     /locus_tag="VGA15_16065"
     CDS             1143..>1823
                     /locus_tag="VGA15_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX9589247.1"
                     /translation="MNRRDLIKTFAAIAPLTVAGRVWAAPATDARLLVVFLRGAYDAA
                     NVVVPVSSDFYHSSRPTLGIAKPDVGNPNSALSLNPDWGLHPALRDSIYPLWAKREIA
                     FLPFAGTSDDLSRSHFETQDTIELGQSTGGSRDYRSGFMSRLATELTRVKPISFTDQL
                     PLIFRGKSQIPNTGINSVGKPGVDDRQARLIKNMYAQSDLASSVSEGFRVRDDVYKSI
                     SEEMTAANR"
BASE COUNT          365 a          544 c          551 g          363 t
ORIGIN      
        1 caaggccaac attcgtatcg tggtgggtga ttatgaggat cgcgcgatca gaccctatgc
       61 gctgggcaag ttccgcgatc tcctcgtcgc cacgctgcat catccggcga tgctgcgcta
      121 tctcgacaat gccgataacg ctgccgggca tctcaacgag aactacgcgc gcgagatcat
      181 ggagctgcac accatgggcg tcggttccgg ctacgcgcaa tccgatgtcg aggcgctcgc
      241 acggatcttg accggcgtcg gaatcgacct gaagccggaa gacccgaagc tcaagccgga
      301 actgcaatcg cagcttgtgc gtgagggcgc ttttgaattc aatcctgccc ggcatgacta
      361 tggcgacaag actttcctcg gtcacgcgat caagggccgt ggactggccg aagtggacga
      421 ggcgatcgat atccttgtcc gccatccggc gaccgcgacc catctttgcc ggcagatcgc
      481 gacctatttt gtatcggaca atccgccgga gccgctggtg cagcggatgg cgcaaacctt
      541 caaggcgacc gacggcgata tcgcggcggt gctcgctgcg atggttcatg cgccggagtt
      601 cacggcgtcg ttgaagcctg gcgccaggtt caaggatcct gtgcaatacg tattctcggc
      661 ggttcgcctc gcttacgacg acaaggtgat cctgaatacg tcatcgatcc agggctggtt
      721 gaaccgtctg agcgaaggat tgttcaatca tgatacgccg gacggttatt cgatgacctc
      781 ggcgtcgtgg aatgggccgg gacaaatgat gctgcgcttc gagatcgcgc ggcaaatcgg
      841 ttcgggttcc tcaggcctgt tcaagccgga tgtgccgaac gcggtcgatc agccggcttt
      901 cccgctgatt cagaactcgc tctacttcaa tggactgcgg cagaccttgg gttccccgac
      961 gctggccgca ctcgatcagg cggtatcgcc acaggactgg aacacgctgt ttctgtcgtc
     1021 gcccgagttc atgcggtaga gcgttttcaa gcgaagcatg ccctcggact tgatccgggg
     1081 gtggagaccg gatcgcgtca agaaaacgcg accaatcaag aagccagcag gaggatcgca
     1141 ccatgaatcg ccgtgatctg atcaagactt tcgccgcaat agctcccctg acggtggctg
     1201 gccgcgtctg ggcagcgcca gccaccgatg cgcggctgct ggtggtgttt ttgcgcggcg
     1261 cctacgatgc cgccaatgtc gtggtgcccg tgtcgagcga cttctaccat agttcgcggc
     1321 ccactctcgg cattgccaaa cccgacgtcg gtaatccgaa ctcggcgctg tcgcttaacc
     1381 cggactgggg actgcacccg gcattgcgcg acagcatcta tccgctctgg gcaaagcggg
     1441 aaatcgcgtt tcttccgttt gcaggcacaa gcgatgatct gagccggagc cacttcgaaa
     1501 cccaggacac cattgaactc ggacaatcga ccggcggctc ccgcgactat cgctcgggct
     1561 tcatgagcag gctcgccacc gagctgacgc gggtcaagcc gatttccttc accgaccagt
     1621 tgccgctgat ctttcgcggc aagtcgcaga ttccgaatac cgggataaac agcgtgggca
     1681 aaccgggggt cgacgatcgc caggccaggc tgatcaagaa catgtacgcg caaagcgatc
     1741 tcgcgtcgtc ggtttcggag ggatttaggg ttcgggacga tgtctacaaa tcgatatccg
     1801 aagagatgac cgcggccaat cgc
//
LOCUS       DASWGM010000563        11824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2423481,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000563 DASWGM010000000
VERSION     DASWGM010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2423481"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VGA15_16070"
     CDS             <1..714
                     /locus_tag="VGA15_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9589248.1"
                     /translation="LGALAGGFVSGLAGFGTALMALGIWLYVVPPSIAVPLVLIGSVL
                     GQTSTLPSFWRSIDFKLVWPFLIGGLAGVPLGTLLIAHADPRIFKLGVGVLMLVFPAA
                     LYFNRTPMALRFGGRLADATIGFAGGILGGLAGLSGPLPILWASVRGWGKDERRGVFQ
                     TFNWTVLSAALCLQAGTGFITGQVVWLALLVLPATLFGAWLGARTYRVLSDRNFHDVV
                     LGLLFLSGVGLVWSGLGLR"
     gene            780..1688
                     /locus_tag="VGA15_16075"
     CDS             780..1688
                     /locus_tag="VGA15_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9589249.1"
                     /translation="MPSPKLSPEHLGLLLGFIGMAIFGGTLPATRLAVSGFDPFALTA
                     LRTAIAGLCALILLLVLRRPLPPRRLWFQLAIAMLCLSILFPFLMVLAVLTVDASHGG
                     VVLGILPIATALVALAITHERPKPLFWVASVAGASLVIAFALRQGGGALSAGDLQLFA
                     AVAIAAIGFTFSGRLTAEMPGWEVISWVQVIALPISLPAAALTLPADLTHIALKPWLA
                     LLYVAVFSQWIGFFAWNAGMAMGGITRVSQVQLLQPFITFALAALFNDETITLQILLF
                     AAAVVATVAIGTRTRVRKTPVVQRSP"
     gene            complement(1909..3744)
                     /locus_tag="VGA15_16080"
     CDS             complement(1909..3744)
                     /locus_tag="VGA15_16080"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEX9589250.1"
                     /translation="MISGVNGITASTLNLNNAGTVTATARGQAAIAGGTVNITANSGT
                     IQALAAGGIAIEEEIAATVNNSSSGVIKGDKFGIQTTTANINNNAGTIEATGTDGNAI
                     LANTVNVISNAKTIEATGTSGIAIHAFDTAALANMRTGTISGNIAIQAGGVGGIGSTI
                     VNAGAIVSTAGASGTAIKLSAAADTLTLLSGSKIVGIVDMGDVAVVGNDTVNVVAVAP
                     SSKVSSLTTAAELPTLINFHGKLNTTFSGGGFNGPLVQAGNQLATLDPTALAQADRTL
                     MDFTSGVSSLVQGRLNGVSPSSNGAMMAMSYAPEVNSSGPFAKAPARVSGWLSPAPIT
                     VWANSFGGQRTQDATAETLRATSTAFGGAIGIDRKVRPDWLVGAFIGGGSGSLSVDLS
                     SQTVNTDYVFAGAYSRFEWASHFFDFTLQGGNASNKSDRLVLNNLAAGGMERANASYN
                     GWYISPEVAYGFRQDIGNGYVLTPTARVRYVAGFFDGYNETGSAQGLRIGSRTLQDFE
                     ERGELDVSRVTTFFGGDHSLKTNLHGGVIALQRAGDTNVNAVLIGQNLAFATPGKGST
                     VGWVAGAGFDYHVNTNVAVFGAVEGIAMSDQSRIITAKGGVRAAF"
     gene            complement(3797..4243)
                     /locus_tag="VGA15_16085"
     CDS             complement(3797..4243)
                     /locus_tag="VGA15_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589251.1"
                     /translation="MTTSERESSRTEVALKVLARASVGEKSGVLAKMHVAGPKLMLRR
                     HAMMLALLIAPFAAVGQAQAACLPPSTASNNPNVACTGDTKNSSPDHLNGYGTINDNN
                     NTYYINAGATVTGDRSGIVLGTGGDCQQFRRHHGYKSGWCRCSQFC"
     gene            complement(4538..5680)
                     /locus_tag="VGA15_16090"
     CDS             complement(4538..5680)
                     /locus_tag="VGA15_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX9589252.1"
                     /translation="MIPEAEILSDLIGTIYDTTLDRALWSDVLRRSAEFVGGSAAAIF
                     SKNPTAGNGFVYYESGVDPYYRQLYFEKYVDLDPATTGHYFADVGQPIAIADFMPYSE
                     FLETRFYKEWVHPQGLVDLVSAVLDKSVTSAALFGVFRYQRHGIVDDETRRRMRLIVP
                     HIRRAVLIGNVLDLNAAENTAFADTLSGLAAGVFLVDAGARIVFANRSGEIMLDEGNI
                     LCQRNRVLTTVDPKVGTTLPDVIALARDGDAAVGANGVAVPLSSPPGEPWLAHILPLT
                     SGARQHAGIVHSAVAAVFVHQASLETPSSMETMSKLYRLTPSELRVLAAVSEVGGIAA
                     IAEVVGIAEATVKTHLQRLFAKTGTNRQTDLVKLVATHTSPLRQVP"
     gene            complement(5998..7107)
                     /locus_tag="VGA15_16095"
     CDS             complement(5998..7107)
                     /locus_tag="VGA15_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9589253.1"
                     /translation="MHGTRKLPDLIEEIYDAALEPALWNDVVVSINDFIGSRACGLIS
                     KDTMSQFGLTHHYCGVDPHYIQLYADTHSRFDPLTTLPSLGQVVSIPDLVRYDEYRRG
                     PFYQEWLRPQGCVDVANVVLEKPSSNCAVLLTVLPGAQMLDDEMRRRIALIAPHVRRA
                     LLINKAIDLRQSEAATFADTLDGLSAAIFLVDAGCRIMHANVAGDEMLRANDFLRSIG
                     GQLVAGSAQGNRTLREIVADGGGAPGGKGAALPLTAQDGERYVMHVLPLTAAARAATG
                     IAYKAVAALFVRKVALDNPVGELVARHFELTPAELRVLLSIVEIGGVPETAAALGVAE
                     TTVKTHLHRVFSKTGASRQADLVKLAAGFSSPLAG"
     gene            complement(7383..9194)
                     /locus_tag="VGA15_16100"
     CDS             complement(7383..9194)
                     /locus_tag="VGA15_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589254.1"
                     /translation="MTPMSAEGNKRPAAIRVVIPFVFRHWLNQPATAATVAGGLLGAT
                     VADLFMPLFSGHLVDAVTLGASDPAARRAAFTAFGAIVALGLVSMTLRLVGLHAIVPF
                     TLRIMSDVSRDAFMRVQRFSTDWHANSFAGSTVRKVTRGMWALDLLNDTILMALLPSL
                     AVLLGSMILLGLHWSSLGLVIAVGALIYVSMTVVFSTRYIAPAARISNAWDTKVGGTL
                     ADALTCNAVVKSFGAEAREDARLARVISRWRTRVRRTWLRYNYTSTAQLAVLLCVRGS
                     VVGGALLLWIAGRASPGDVTYVLTSYYIIHAYLRDVGMHINNLQRSVNDMEELVAIHD
                     EAIGIADVAGASPINVHGGRIVFEDVTFHYGGHRVALYDGLSVDIRAGERVGLVGRSG
                     SGKTTFVKLVQRLYDVSGGRILIDGQDIAKATQYSLRSQIAIVQQDPILFHRSLAENI
                     AYGRPGASTASIEQAARLANAHEFILRLPKGYGTLVGERGVKLSGGERQRVALARAFL
                     ADAPVLILDEATSSLDSESEALIQQAMERLMKGRTSIVIAHRLSTVRSLDRILVFDRG
                     EIVEQGTHAALATRTGGIYRGLFERQATEFGRVSAAE"
     gene            complement(9520..10866)
                     /locus_tag="VGA15_16105"
     CDS             complement(9520..10866)
                     /locus_tag="VGA15_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9589255.1"
                     /translation="MKDLTQGSIVSHISTMAPQIFAGMIMIMLCQLIDLYFVSDLGDA
                     AIAGVGAAGNAGFLINALMQVLSVGTVALISHAAGRKDQADANLVFNQSLGLSAACGL
                     LTLIAGSALARSYMRSIAADHVVVEAGTTYLLWFMPALALQFAILVMASALRGTGIVR
                     PTMIVQTSTVILNIILAPVLIAGWGTGWALGVAGAGLASSIAIAAGGMMMWIYFHSYE
                     HYVVINRELWRPQIAQWKRILNIGLPAGGELAILFVSMAVIYYALSDFGAAAQAGFGI
                     GTRLLGVVHMPAMSIAFAAGAIAGQNFGAGNGERVKETFRSVLLLGTAVMLAFTVLAQ
                     WRPELWLGAFTKDPETIAVGALFLRMISLNLVAQGLIFVCSSMFQGLGNTKPALLSSG
                     ARLIMYGLPLIWLSTKPGFRIEQVWYLSIATTTLQAAFSLWLLRLEFRKRLVPLVQ"
     gene            complement(11182..>11824)
                     /locus_tag="VGA15_16110"
     CDS             complement(11182..>11824)
                     /locus_tag="VGA15_16110"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX9589256.1"
                     /translation="FLRDYLYIPLGGNRLGEQRRYINLLVTMLLGGLWHGAGWNFLVW
                     GGLHGIYLSINHLWRGWRGSKPEVSIAGLMVKGACWAITFFAVVVAWVFFRAKTAAGA
                     WQMLRGLFGIEAGSSAYASSGILRLMDLPMLVGEERLLLIGGGAVALALAMALCLPNV
                     PQLFRYREYRRAPERAAWVRWRPNAAWALLSALALAISLFGMWQRLEFLYFQF"
BASE COUNT         2180 a         3720 c         3694 g         2230 t
ORIGIN      
        1 ctcggcgcgc tcgccggcgg cttcgtgtcc ggccttgccg gcttcgggac cgcgctgatg
       61 gcgctcggaa tctggctcta cgtggtgccg ccttcgatcg cggtgccgct ggtcctgatc
      121 ggttccgtcc tcgggcagac ctccacgctg ccgtcgtttt ggcggtcgat cgacttcaag
      181 ctggtctggc cattcctgat cggcggcctc gccggcgtgc cgctcggtac cctgttgatc
      241 gctcatgccg acccccgcat attcaaattg ggcgtcggcg tgctgatgct ggtgtttcca
      301 gcggcgctct atttcaaccg caccccgatg gcgctgaggt tcggcggccg attggcagac
      361 gccacaatcg gatttgcagg cgggattctg ggcggcctcg ccggtctatc cggcccgctt
      421 ccgatattgt gggcaagcgt gcgaggctgg ggcaaggatg aacggcgcgg cgtttttcaa
      481 accttcaact ggaccgtgct gtcggccgcg ctgtgcctgc aggcggggac cgggttcatc
      541 accgggcagg tggtttggct cgcgttgctt gtcctgccgg cgacgctgtt cggcgcctgg
      601 ctcggcgcac ggacttatcg cgtgttaagc gatcggaatt tccacgacgt cgttcttggc
      661 ctgttgtttc tgtcaggcgt cggtctcgtc tggagtggcc tcgggttgcg gtagccttgc
      721 atggccacgc ggagcgtgcg aaagctgtac gcgcgcgcga accggctacc tcagacacca
      781 tgccttcccc taaattgtct cctgaacatc tgggtctgtt gctgggtttc atcggcatgg
      841 ccatcttcgg cggaacgctg cccgcgacgc gccttgcggt ttcgggattc gatccgtttg
      901 cgctcacggc gcttcgcacc gcgatcgccg ggctgtgcgc gttgatcttg ttgctggtgc
      961 tgcgacggcc gctgccgccg cgccgactgt ggttccagct cgccatcgcg atgctgtgcc
     1021 tgagcatcct gtttccgttc ctgatggtgc tggcggtgct gaccgtcgat gcttcgcatg
     1081 gcggcgtggt gctggggatt ctcccgatcg ccaccgcgct ggtcgcgctc gccattactc
     1141 atgagcggcc aaaaccgctg ttctgggtcg cgtccgtcgc cggcgcgtcg ctggtgatcg
     1201 cgttcgcgct gcgccagggc ggcggcgcgc tctccgccgg cgatctgcag ctgttcgccg
     1261 cggtcgctat cgcggcgatt ggttttacct tctccggacg gctcaccgct gagatgccgg
     1321 gatgggaggt catcagctgg gtgcaggtga tcgcgctgcc gatctcactg cccgcggctg
     1381 cgctgaccct gccggcggat ctgacgcaca tcgccttgaa gccgtggctt gcgctgctct
     1441 acgtcgcggt gttctcgcag tggatcgggt ttttcgcgtg gaatgccggc atggcgatgg
     1501 gcggcatcac gcgcgtatcg caagtgcaac tgctgcagcc cttcatcact tttgcgctcg
     1561 cggcattgtt caacgacgag acgattacac tgcaaatcct gctgttcgcc gcggccgtcg
     1621 tcgccaccgt ggcgatcggc acacgcacgc gggttcggaa aacgccggtc gttcagcgtt
     1681 caccttgagc gccgtcgggc tgacgcatca gcccgccaac ggactggaag atcgatttgc
     1741 cggtcggtgc cggtcttcgc aaacagccgc tgcagatagg ttttggcaga gtcgcgcata
     1801 cgcctcttca cgcgccgaca aggtgcacgg catggcacat cctccgatct gaggatggcc
     1861 gcgggttgat gcgcgaccat ctccagttcg aaggatggag tcgtagcctc agaacgcggc
     1921 gcggacgccg cccttggcgg tgatgatgcg gctctggtcg gacatcgcga tgccctccac
     1981 cgcgccgaac accgcgacgt tggtgttgac gtgatagtcg aagcccgcgc cggccaccca
     2041 gccgaccgtg ctgcccttgc ccggcgttgc aaacgccagg ttctgaccga tcagcacggc
     2101 gttgacattg gtgtcgcccg cccgctgcag cgcgatcacg ccgccatgca gattggtctt
     2161 caggctgtgg tcgccgccga agaaggtcgt caccctggac acgtcgagtt cgccgcgctc
     2221 ctcgaaatcc tgcagcgtgc ggctgccgat cctcaagccc tgcgccgaac cggtctcatt
     2281 gtagccgtcg aagaagcctg cgacatagcg cacccgcgcc gtcggcgtca gcacatagcc
     2341 gttgccgata tcctggcgga agccgtaggc cacctccggg ctgatgtacc agccgttgta
     2401 gctggcgttc gcccgctcca taccgccggc cgcgaggttg ttgagcacca gccggtccga
     2461 cttgttcgac gcgttgccgc cctgcagggt aaaatcgaaa aagtgcgagg cccattcgaa
     2521 gcggctgtag gcgccggcga acacgtaatc ggtgttgacc gtctgcgagc tcaaatcgac
     2581 cgacagtgaa ccgctgccgc cgccgatgaa ggcgccgacc agccagtctg gccgcacctt
     2641 gcggtcgatg ccgatggcgc caccgaacgc cgtcgaggtc gcgcgcaagg tttctgccgt
     2701 cgcatcctgg gtgcgctggc cgccaaagct gttggcccac accgtgatcg gcgccgggct
     2761 caaccagccg gaaaccctgg ccggcgcttt cgcgaacggc ccactgctgt tgacctccgg
     2821 cgcgtagctc atcgccatca tcgcgccatt ggaagacggc gacacgccgt tgagacggcc
     2881 ctgcaccaat gacgataccc cactcgtaaa atccatcagg gtgcggtcgg cctgcgccag
     2941 cgcggtcgga tccagcgtcg cgagttgatt gccggcctgc accaagggac cgttgaaacc
     3001 gccgccggag aacgtggtgt tcaacttgcc gtggaagttg atgagcgtcg gcagttcggc
     3061 ggcggtcgtc agcgacgata ccttgctgct cggcgcgacg gcgacgacat tcacggtgtc
     3121 attgccaact accgcgacgt cgcccatgtc gacgatgccg acgatctttg agcccgacaa
     3181 cagcgttagc gtgtcggccg cagccgacag tttgattgct gtgccgctgg cgcccgcagt
     3241 gctgacgatg gcgccggcat tgacgatggt gctgccaatg ccgccaacgc cgccggcctg
     3301 aatggcgata ttgcccgaga tcgtgccggt gcgcatgttg gccaaggcgg cggtgtcaaa
     3361 ggcgtggatc gcgatgccgc tcgtgcccgt tgcttcgatt gttttggcat tcgagatgac
     3421 attgacggtg ttggccagga tagcgttgcc gtccgtgccc gtcgcttcga tggtgccggc
     3481 attgttgttg atatttgccg tcgtggtctg gatgccgaat ttgtcgccct tgatgacgcc
     3541 ggaactggaa ttgttcacgg tggcggcgat ttcctcttcg atggcgatgc cgcccgcggc
     3601 gagtgcttgt atcgtgccgg agttggcggt gatgttgacg gtgccgccag caatggcggc
     3661 ctggccccgg gccgtcgccg tgaccgtgcc ggcgttgttg agattgaggg tgctagcggt
     3721 gatgccgttg acgcccgaga tcacggcggc gctggtgctg gtgctgatat tgtttacagt
     3781 gagattggca gcttggtcag caaaattggg agcatcgaca ccagcctgat ttgtacccgt
     3841 gatggcgccg gaattgttga cagtcccccc ccgtaccgag cacgataccc gaacggtctc
     3901 ccgtcaccgt cgcaccggcg ttgatatagt aggtattgtt gttgtcgttg atggtgccgt
     3961 agccgttgag gtgatccgga ctgctatttt tggtgtcgcc cgtgcaggca acattcgggt
     4021 tgttgctggc cgtcgatggt gggaggcagg ccgcctgcgc ctgcccgacc gctgcgaacg
     4081 gcgcgatcag cagcgccagc atcatggcgt gtcgccgcaa catcagcttc gggccggcca
     4141 catgcatttt tgccaacacg ccggattttt cgccaacgct ggcgcgagcc aacactttca
     4201 aggcaacctc cgtccgagac gattcacgtt cggacgtagt catgcgggaa agctcagggt
     4261 gcgcgcgtca accggcgggt tgatccggcg ccgaatggcg gctccgcgag gcggatggtc
     4321 ccgcagcgtt gcacgatggc aacggtcatg tcgtcccggc gaaccaacgg gtccggctat
     4381 tgccggcccg atggcaggct ctgggaccca taaccaccag cgtttgttgt tgtgccgagg
     4441 cgtcggctcc gtttttcaat tgcaactcca cggcgtatgg gtcccggcgt tcgccgggac
     4501 gacgctgatc ttggagttca cgcctgggag cctgctctca ggggacttgc cgcagcggac
     4561 ttgtgtgggt ggcgaccagt ttgacgaggt cggtctgccg gttggtgccg gtcttcgcaa
     4621 acagacgctg cagatgggtt ttgacggtgg cttccgcaat gccgacgact tcggcaatcg
     4681 ccgcaatgcc gccaacctcg ctcaccgccg cgagcacgcg cagttcgctc ggcgtcagtc
     4741 ggtagagctt cgacatggtc tccatcgatg acggtgtttc aagcgaagcc tggtgaacga
     4801 acaccgccgc cacggcggaa tggactattc cggcatgctg cctcgccccc gaggtgagcg
     4861 gcagaatgtg ggcaagccag ggttcccctg gcggcgaaga gagcggaacc gcgaccccat
     4921 ttgctccgac cgctgcgtcc ccatcacggg ccaacgcgat gacatccggc agcgtcgtcc
     4981 ctaccttcgg atcgacggtc gtcagcacac gatttctttg acaaaggata tttccctcgt
     5041 cgagcatgat ctctccggat ctgttggcaa atacgatgcg ggcacctgca tcgacgagaa
     5101 agacaccggc agcgaggcca ctcaacgtat ctgcgaatgc cgtgttctcc gccgcgttga
     5161 ggtcaagcac gtttccgatc agcactgccc ggcggatgtg cggaacgata aggcgcatac
     5221 gccggcgcgt ttcatcgtcg acaatcccgt gacgttggta gcgaaacacc ccgaacagtg
     5281 ccgcgctggt taccgattta tcgagcaccg ccgataccaa atccaccagg ccctgtgggt
     5341 gaacccactc cttgtaaaat cgcgtttcca ggaactcgct gtacggcatg aaatccgcta
     5401 ttgcgatcgg ttgcccgaca tcagcaaagt aatggcctgt tgtggcaggg tcgaggtcta
     5461 catatttttc gaaatagagt tgccgatagt acgggtcgac gccggactcg taatagacaa
     5521 agccgttccc ggccgtcggg tttttggaaa agatggccgc cgccgagccg ccaacgaatt
     5581 ccgccgacct tctcaacacg tcggaccaca gggcccggtc gagcgtcgtg tcgtagatgg
     5641 ttccgatcag atctgatagg atttctgctt ccgggatcat tgggtctgtc tgatcatcca
     5701 ttcagctaat attgcattgg ccagaggggg gcgcatcaat cgatcagttg atgcggtgct
     5761 tgcgcgcctg agacagatca cgaaatccag agccttttcc gttccggtcg aatcggaacg
     5821 gggctccatt ttcttgttgg cccgcaactc gtccccgaac cgcgcggtcg ggatacgctg
     5881 gagtctgatt caaccaagtt gaatcggact ccagcgtatc tttttgtttg agcatgatct
     5941 tttcggaaaa ccggtttcca cttttccgga tcatgctctc gcggttcagg gctggtatca
     6001 gcccgccaac ggactggaaa atccggcggc gagtttgacg agatcggcct ggcgactggc
     6061 tccggtcttg gagaacacac ggtgcaaatg ggttttgacc gttgtttccg cgacacccaa
     6121 agctgccgcc gtctccggca cgccgccgat ctcgacgatg gaaagcagca cacgcagctc
     6181 cgccggcgtc agttcgaaat ggcgcgcgac gagttcgccg acggggttgt ccagcgccac
     6241 ctttcggacg aacaaggcgg cgacggcctt gtaggcgatg ccggtggcgg cccgagccgc
     6301 cgctgtcaac ggcaacacgt gcatgacgta acgttcgccg tcctgcgcgg tcagaggcag
     6361 ggcggcgccc ttgccgcccg gcgcgccgcc accatcggcg acaatttcgc gcaaagtccg
     6421 attgccctgc gcgctgccgg cgacgagttg cccgccgatg gagcgcagaa aatcgttcgc
     6481 ccgcagcatt tcatcgccgg ccacattggc atgcattatc cggcagccgg catcgacgag
     6541 gaatattgcg gcgctgagac catccagcgt gtcggcaaat gtcgcggctt cggattgcct
     6601 gagatcgatt gccttgttga tgagcagcgc ccggcggaca tgcggtgcaa tcagcgcgat
     6661 ccgccgtcgc atttcatcgt cgagcatttg cgcgcccggc agaaccgtaa gcagaaccgc
     6721 gcaattcgag ctcggttttt cgagcaccac gttggcaacg tcgacacagc cttgtggccg
     6781 caaccactcc tgatagaagg gtccccggcg gtattcgtca tagcgcacga gatccggaat
     6841 gctcacgact tgccccagcg acggcaaggt cgtgagagga tcgaatctgg aatgggtgtc
     6901 cgcataaagc tggatgtaat gaggatccac accgcaataa tgatgcgtaa gcccaaattg
     6961 gctcatcgta tccttcgaga tgagtccgca agcccggctt ccgatgaaat cgttgatact
     7021 gacgacgaca tcattccaca atgcgggttc aagagccgcg tcatagattt cctcgatgag
     7081 atcgggaagt tttcgggttc cgtgcatagg gctgatccgt cgcgtatttg gccaagtagc
     7141 gtctcactga gcggcctcat cctccgatcg gaggataaaa caattaagcc gccacggtag
     7201 atgggtctgt cggcggcatt tgaggttcat cgctatccgc caagtatgtt tggctaaccc
     7261 caaaatgagg tcgctattcc cggttcgcgt cggaacctgt catcggaccg gcccgccatc
     7321 gggtgcgcat tcgcgcgacc cgttggcgcg aacccgccgg atgcgctccg atgacctgcc
     7381 gcctactccg ccgccgaaac gcggccgaat tccgtcgcct ggcgttcgaa cagcccgcga
     7441 tagatccctc cggttcgggt ggcgagcgcg gcgtgggtgc cctgctcgac gatctcgccg
     7501 cggtcgaaca ccagaatgcg atcgaggctg cgcaccgtcg acagccggtg cgcgatcacg
     7561 atcgaggtgc ggcccttcat caagcgttcc atcgcctgct ggatcagggc ttccgactcg
     7621 gaatccaggc tcgaggtcgc ctcgtcgagg atcagcaccg gggcgtcggc gagaaacgcc
     7681 cgcgccagcg ccacgcgttg ccgctcgccg cccgacagtt tcacgccgcg ttcgccgacc
     7741 agcgtgccat agcctttcgg cagccgcagg atgaactcat gcgcattggc gagccgcgcg
     7801 gcctgctcga tcgaagccgt gctggcgccc ggccggccgt aggcgatgtt ctcggccaat
     7861 gatcggtgaa acaggatcgg atcctgctgc acgatggcga tctggctgcg cagcgaatac
     7921 tgcgtcgcct tcgcaatgtc ctgaccgtcg atcaggattc gtccgccact gacatcgtaa
     7981 agccgctgca ccagcttgac gaaggtggtc ttgccggagc cggagcggcc gaccaggccg
     8041 acgcgttcgc cggcgcggat atccacggac aggccgtcat acagcgcaac gcgatggccg
     8101 ccgtagtgga aggtcacatc ctcgaacacg atgcgtccgc cgtggacatt gatcggcgac
     8161 gcgccggcga catcggcaat gccgatcgcc tcgtcgtgga tcgcgaccag ctcctccatg
     8221 tcgttgaccg agcgttgcag gttgttgatg tgcatgccga catcgcgcaa ataggcgtgg
     8281 atgatgtagt agctggtcag cacataggtg acgtcgcccg gcgaggcgcg acccgcgatc
     8341 cacagcaaca acgcgccgcc gaccacggag ccgcggacgc agagcagcac cgcgagctgc
     8401 gcggtactgg tgtagttgta gcgcagccag gtccggcgca cgcgcgtgcg ccatcggctg
     8461 atgacgcgcg ccagccgcgc gtcctcgcgc gcttcggcgc cgaacgattt caccaccgcg
     8521 ttgcaggtca aggcatcggc cagcgtgccg ccgaccttgg tgtcccaggc gttggagatg
     8581 cgcgccgccg gcgcgatata gcgcgtcgag aacaccaccg tcatcgacac gtagatcagc
     8641 gcgcccaccg caatcacgag gccgagcgaa gaccagtgca ggccgagcag gatcatcgat
     8701 cccagcagca ccgccagcga tggcagcagc gccatcagaa tggtgtcgtt gagcagatcg
     8761 agcgcccaca tgccgcgggt gaccttgcgc acggtcgagc cggcgaagct gttggcgtgc
     8821 cagtcggtcg agaagcgctg cacgcgcatg aaggcgtcgc gcgacacgtc ggacatgatc
     8881 cgcagcgtga acggcacgat cgcatgcagc ccgacaagac gcagcgtcat cgagaccagg
     8941 ccgagcgcca cgatggcgcc gaaggccgtg aacgccgcac ggcgcgcggc cggatcggat
     9001 gcgcccagcg tcaccgcgtc gaccagatgt ccggaaaaca gcggcatgaa caggtcggcg
     9061 acggtcgcgc caagcaggcc gccggcaacg gtcgcggcag tagccggctg attgagccag
     9121 tggcgaaaca cgaacgggat caccacgcgt atcgcggcgg ggcgcttgtt gccttctgcg
     9181 gacatcggcg tcatccggct acgctcgcgc gcggaccggc tccgttggtt gacgagcgac
     9241 ggcatggccg tacgcacgcg tcgttgaaaa aatggcggtg acttacggga gcaagggaga
     9301 tcgggacgtc aggcccgacc gatgctggcg gaaacggccc tcaaagggcc acgcgggcat
     9361 ccgatactgg cttcggacgc gagggcgcga tctgcgaata catcgaaatc atgcaaatcc
     9421 tccccggttc gagcgacgat ccgctgctta taaatgcatc atgggcgttt tgcaatatgc
     9481 cggcaccaaa gtcttgcaag gaaactgccg tgccccgcct cattgcacga gcggcaccag
     9541 ccgttttcta aactccagac gcaacagcca caggctgaac gccgcttgca gcgtggtcgt
     9601 cgcaatcgat aaataccaga cctgctcgat gcgaaaaccg ggcttggtcg aaagccagat
     9661 caacggcagc ccgtacatga tcaggcgcgc gccggagctt aacagcgccg gtttggtgtt
     9721 acccaatccc tgaaacatgc ttgaacacac aaagatcaac ccctgcgcga cgaggttcag
     9781 agagatcatc cgcaggaaca gcgcgccgac ggcgatcgtt tcggggtcct tggtaaaggc
     9841 tccgagccac aactccggcc tccactgtgc cagcaccgtg aaagccaaca tcacggcggt
     9901 gccgagaagc aggacgctcc tgaaggtttc cttcacccgc tcgccattgc cggcgccgaa
     9961 gttttgcccg gcgatggccc cggccgcgaa tgcaatggac atcgccggca tatgaacgac
    10021 gcccagcagg cgcgtgccaa tgccgaagcc ggcctgagcc gccgcgccga aatcgctgag
    10081 cgcataatat atgacggcca tcgagacgaa cagaatggcg agttcgcctc cggcaggcag
    10141 cccgatgttc aatatgcgct tccattgcgc aatttgcgga cgccacagtt cgcgattgat
    10201 gacgacgtag tgttcatagc tgtgaaaata tatccacatc atcatcccgc cggctgcaat
    10261 cgcaatcgag ctcgcaagcc cggcgccggc aacgcccaaa gcccagcctg tgccccagcc
    10321 cgcgatcaga actggagcaa gaatgatgtt gaggatcacc gtgctggtct ggacgatcat
    10381 ggtgggccgc actatcccgg tgccgcgcaa ggcagaggcc attaccaaga tagcgaactg
    10441 cagcgccaat gccggcatga accaaagcag atacgttgta cccgcctcga cgacgacatg
    10501 gtctgccgca atcgagcgca tgtagctgcg cgcgagtgca gatccggcaa tcagcgtcaa
    10561 caacccgcaa gccgccgaca agccgagcga ctgattgaag accagattgg catcggcttg
    10621 atccttgcgc cccgcggcat gcgagatcaa cgcaaccgtg cctacgctca gcacctgcat
    10681 tagagcgttg atgagaaagc cggcattgcc cgccgcgccg acgcccgcga tcgccgcgtc
    10741 gcccagatca gaaacaaaat acaaatcaat cagttggcag agcatgatca tgatcatgcc
    10801 ggcaaagatc tgcggagcca tggtggagat atggctgacg atagagcctt gcgtgagatc
    10861 tttcatatca gcacatccga tcgcgcttgc gcgccgaccg gctccgttgc gtgacgcgcg
    10921 ctggcacagg gccgaacgac aggcgtcgtt gaaaaaatgg cgatgactta aggggaaact
    10981 ttggaagatc gggacgtcag gcccgatcga tgctgacgga ggtggccctc agagggccag
    11041 ccaagcgtcc ggcatgagca tcggacgcga gggcgcgatc tgcgaataca tcgaaatcat
    11101 gcaaatccct ccccggttcg agcgacgacg cggtgcttat agatgcgtcg cgggcgattt
    11161 gcaatatggc gagcggcgcg gtcagaattg aaaatacaaa aattccagcc gctgccacat
    11221 gccgaacagc gagatcgcga gcgccagcgc ggacaaaagc gcccacgccg cgttcggccg
    11281 ccatcgtacc catgctgccc gctccggcgc ccggcgatat tcgcggtaac gaaacagctg
    11341 cgggacattg ggcaggcaaa gcgccatggc cagcgccagc gcgaccgcgc cgccgccaat
    11401 cagcaataac cgctcctcgc ccacaagcat cggcaaatcc atcagccgca ggatgccgga
    11461 ggatgcgtaa gcggagctgc ctgcctctat tccgaacagg ccccgcagca tttgccatgc
    11521 cccagccgcc gttttggcgc gaaagaacac ccaggcgacc acgactgcga agaaggtgat
    11581 agcccagcat gcgcctttca ccatcaatcc ggctatcgat acctccggtt tgctgccacg
    11641 ccatccgcgc cagagatgat tgatgctgag atagatgccg tgcaggccgc cccagaccag
    11701 aaaattccag cccgcgccat gccagagccc gccgagcagc atcgtgacca gcaaattgat
    11761 atagcgccgc tgctcgccga gccggttgcc gccgagcgga atgtagaggt agtcgcgcag
    11821 gaag
//
LOCUS       DASWGM010000564         4038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2561698,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000564 DASWGM010000000
VERSION     DASWGM010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2561698"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VGA15_16115"
     CDS             complement(<1..1083)
                     /locus_tag="VGA15_16115"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9589257.1"
                     /translation="MPAAGLGLLVLVALGIVLTGLPAFVILIGVASIGAALAVLTDTV
                     PLSLLAALPSRLINLLENDLLQALPLYVTMGLLLDRLPIADALYRAGLSLLPRRPSAP
                     LVSGMALGALLGPMNGSVGASVLALARVVAPRLAESGVPKPTQFAAVAVASTLGVVIP
                     PSLVLILLGDAMLSAHTIAVTSTGRADRVINTQDVFHGALAPAAIFLALCFALSWRAG
                     RRIPPSAGAPPVKLTRKQAVLAAITLVALLTLLGGVATGYFYAVEAAAMGAFVLLSAG
                     FGTGALRGAVLRGVLHDALAITGALFALLIAATTLTLVLRLLGTDRLVSDWITGIPGS
                     ELVVVAIVLGAIGLSAFVLDAFEIIFV"
     gene            complement(1083..1571)
                     /locus_tag="VGA15_16120"
     CDS             complement(1083..1571)
                     /locus_tag="VGA15_16120"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9589258.1"
                     /translation="MKLARTVSILDRLIAGLLRLAGWLVLPIVVLLFLQWPLRDIFRV
                     YSREANDLGQWIFAIYVAASVTAATRAGTHLGTDAVARFYPGTIRRALTRLGAILLVP
                     WALYVILGSKDIVLGSIRGLEAFPDTNNPGYFLIKTALWILAGLMLAQAAIDIAQPRR
                     NH"
     gene            complement(1711..2634)
                     /locus_tag="VGA15_16125"
     CDS             complement(1711..2634)
                     /locus_tag="VGA15_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9589259.1"
                     /translation="MKSLLPAVAAIAVLAACNQASAQSSSDVLQRLEALEKSNAKLQE
                     SNLDLKQENAALRDRVKRVESTRQTASAPPTAQPGSTAPTMRSAPGYAGTAPVYKATP
                     IAVPAAWTWTGAYVGAFGGYGWGTFFPPVKSDDPSNGAASEVRLRGGFGGVQAGYNLQ
                     VAPHWLLGVEEDVWAGRIAGHRFQPSPNPTINTEIDYGGTVRGRFGYVWDHALFYSTA
                     GILWAVNRVELDFQPGDNNGVSTVSQTHWHFGVALGDGIEVAVLPNVSVKAEYLFQYL
                     TKEQYFSATSSSDVAGMGVHTVRVGVNWLFH"
     gene            2864..3427
                     /locus_tag="VGA15_16130"
     CDS             2864..3427
                     /locus_tag="VGA15_16130"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX9589260.1"
                     /translation="MDGVPAFELSIFLPATFAAALVAGLAGFAFGLIAAATWLHVLSP
                     SDTAALIVGFGLVVPGVAVWKLRRALDPRRLWPFLVGGALGVPLGVAVLEWAQPGRLR
                     VGIGVLLVAYSLYALVRPAQLDRQGPIRTCPRRTLAAATYALQLFVERLVEFFLFLLS
                     GRLLIWLPLTWRHFSCFWLAVAHWSLI"
     gene            complement(3536..3823)
                     /locus_tag="VGA15_16135"
     CDS             complement(3536..3823)
                     /locus_tag="VGA15_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589261.1"
                     /translation="MTAAFEFRGMVRRRHSSRLSRPGLVLFGVTSASGGQFSSDGSSR
                     IGDFEAVCRIELEAIIAKKLDAAYLSDWRQQLAEKMAVALRDSSDIVSPTN"
     gene            3822..>4038
                     /locus_tag="VGA15_16140"
     CDS             3822..>4038
                     /locus_tag="VGA15_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589262.1"
                     /translation="MKMHHIGTFTPGSGIAIGLVMLALSPSPSLAQTHPRHHAYHSSV
                     QPRSSGYGFAPGSALDPEAFAMRNAQRS"
BASE COUNT          730 a         1290 c         1291 g          727 t
ORIGIN      
        1 gacgaagatg atctcgaacg cgtcgagcac gaatgcgctg agcccgatcg ccccgagcac
       61 gatcgcgacc acgacgagct cgctcccggg aatgccggtg atccagtcgc ttaccaggcg
      121 atcggtgccc agcagccgca agaccagggt caaggtggtg gcggcgatca gcagcgcgaa
      181 caatgcgccg gtgatggcca gggcatcgtg gagaacgccg cgcagcaccg cgccgcgcaa
      241 ggctccggtg ccgaacccgg cgctgagcag gacgaatgct cccatcgctg ccgcttccac
      301 cgcatagaaa tagccggtcg cgacgccgcc caagagcgtg agcagcgcga ccagcgtgat
      361 cgctgccagc accgcctgct tgcgggtcaa tttgaccggc ggggcgccag cgcttggcgg
      421 aatgcgccgg ccggcgcgcc acgacagcgc gaaacacagc gcgagaaaaa tcgcggccgg
      481 cgccagcgcg ccgtggaaga cgtcctgggt gttgatcacg cggtcggcac ggccggtgga
      541 agtcaccgcg atggtgtggg cggagagcat ggcgtcgccg agcaggatca gcaccagcga
      601 cggcgggatc accacgccga gcgtgctcgc caccgcgacg gcggcgaatt gcgtcggctt
      661 gggcacgccg ctctcggcga gccgcggcgc caccacgcgc gcgagcgcca gcacgctggc
      721 gccaaccgag ccgttcatcg ggccgagcag cgcgcccagc gccatgccgg agacgagcgg
      781 ggccgacggc cgtcgcggca gcagcgaaag tccggcgcga tagagcgcgt cggcgatggg
      841 caggcggtcg agcaacagcc ccatggtcac gtagagcggc agcgcctgca ataaatcgtt
      901 ctcgagcagg ttgatcaggc gcgaagggag cgcggcgagc aacgaaagcg gcacggtatc
      961 ggtcagcacg gccaacgccg cgccgatcga cgccaccccg atcaggatca cgaaggccgg
     1021 caggcccgtg aggacgatcc cgagcgcgac caggaccagg agaccgaggc ctgccgccgg
     1081 catcagtggt ttcgacgcgg ctgcgcgatg tcgatcgcgg cctgggcgag catcaggccg
     1141 gccagaatcc acagcgcggt tttgatgagg aaatagccgg gattgttggt gtcgggaaat
     1201 gcttcgaggc cacggatcga gccgagcacg atgtccttgc tgccgaggat gacgtacagc
     1261 gcccagggta ccagcaagat ggctccgagc cgcgtcagcg cccggcgtat tgttcccgga
     1321 tagaagcggg cgacggcgtc cgtgcccaga tgggtgccgg cgcgggtcgc ggcggtgacg
     1381 ctggccgcga catagattgc gaagatccac tggccgagat cgttggcctc gcgcgaatag
     1441 acgcgaaaaa tgtcgcggag cggccactgc aggaacagca gcacgacgat cggcagcacc
     1501 agccagccgg ccagtcgcag cagccctgcg atcagccgat cgagaatgga caccgtgcga
     1561 gcgagcttca tcggtcctgc cccagggagg gtacgcaggc ctaatataca gattcctgtg
     1621 gcccgtcagc cgaccaatca acctccccca accgggacgc gctgatccct tgatcgtccc
     1681 cggcgcgctc cgctgcttgc gggagagacc tcagtggaac agccagttca ctccgaccct
     1741 tactgtatgc actcccatcc cggcaacgtc ggacgaactg gtagcagaga aatactgctc
     1801 cttcgttaga tactggaaga gatattcggc cttgacgctg acgttcggca ggacggcgac
     1861 ctcgatcccg tctcctagag caacgccgaa gtgccagtgc gtctgggata cggtcgacac
     1921 cccgttatta tcgcccggtt gaaaatccag ctcgactcga ttgacggccc agaggatccc
     1981 agccgtacta taaaacagtg cgtggtccca cacatagccg aaccggccgc ggacggtgcc
     2041 gccgtaatcg atctcggtgt tgatggtcgg attgggggac ggctgaaacc ggtggccggc
     2101 gattcgtcct gcccagacgt cttcctcgac gcccaataac cagtggggcg cgacctgcaa
     2161 gttgtacccg gcctgtacgc cgccgaaccc gcctcgaagt ctgacctcgc ttgcggctcc
     2221 gttgctcgga tcgtcgcttt tcaccggcgg gaagaaagtt ccccatccgt aaccgccgaa
     2281 cgcgccgacg taggcgcctg tccacgtcca ggcagccggc accgcaatcg gtgtcgcctt
     2341 gtagaccggc gcagttcccg cataaccggg agctgatctc attgttggcg ctgtcgaacc
     2401 cggttgcgcc gtcggcggtg cagatgcggt ctgtcttgtg gattcgacgc gtttcacgcg
     2461 gtcgcggagc gcggcatttt cttgtttcag atcgagattg ctctcctgca gcttcgcgtt
     2521 gctcttctcc agagcttcga gccgctgcaa tacgtcgctg ctcgattgcg ccgatgcctg
     2581 attgcaagcc gccagaacgg caatagcggc aaccgccgga agcaaggatt tcatcacgcc
     2641 ccccgatgtg tgtgattgcg ccctcttggg cgcgcagaaa cctatcactc gcgtggcgat
     2701 ttccgtgtgc caattgcgca acagctgaca ggaatttttc gctggtgata accgaatatc
     2761 gcctgctttc ggttcgctcg tgctagcgtc acctggggat ccgcagcctc cccgtcagat
     2821 ggcacgccat ccaagcgctg ctcgcttcga tgggaggcga gccatggatg gcgttcctgc
     2881 tttcgagctt tcgattttcc tcccggccac ctttgcggcg gcgctcgttg ccggcctcgc
     2941 cggctttgcg ttcgggctta ttgcggccgc gacatggctg catgtgctct cgcccagcga
     3001 caccgccgct ttgatcgtcg gcttcggcct cgtcgtgccg ggcgtcgcgg tgtggaagct
     3061 gcggcgggcg ctcgacccgc gccggctgtg gccgtttctc gtcggcggcg cgctcggcgt
     3121 tccgctcggc gttgcggtgc tggagtgggc gcagccgggt cggttgcggg tggggatcgg
     3181 tgtgctgctg gtggcttaca gcctctatgc cctggttcga cctgctcagc tcgaccggca
     3241 aggaccaatc aggacttgcc cgagaagaac cttagcagcc gcgacatatg ctcttcagct
     3301 ttttgtcgag cgcttggtcg agttcttttt gtttcttctc agcggccgtc tcctgatctg
     3361 gctgcccctg acttggcggc acttcagttg cttttggctg gcggtggccc actggagcct
     3421 catttgaggg ggctctttgc gctaatgcac tgtcaaaggt ggacgctgtg aacaaaatga
     3481 ggcctgatgc cacgacagcc aaccgcatga cacgcactcc tgttccgact ggcttttagt
     3541 tagttggtga cactatatcc gacgagtcgc gcaacgccac agccattttc tcagcgagtt
     3601 gctggcgcca gtcggacaaa taggcggcat cgagtttctt cgcaatgatg gcttcaagtt
     3661 cgatgcggca gacggcttca aagtcaccga ttcgtgaact gccgtcgctt gagaactgcc
     3721 ctcccgatgc agacgttacg ccgaatagga ccaagccggg gcgcgataag cgcgatgagt
     3781 ggcggcggcg gaccatacct ctgaactcga aagcagcagt catgaaaatg catcatatcg
     3841 gaaccttcac gcccggctca ggtatcgcca tcggactcgt gatgctggcg ttgagtcctt
     3901 ctccatcact ggcgcagacc cacccccggc accacgcata tcactcgtcg gtgcagcccc
     3961 ggtcttccgg ctacgggttt gcccctggct ccgcactaga cccggaagca tttgcgatga
     4021 ggaatgcgca acgatctt
//
LOCUS       DASWGM010000565         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_178376,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000565 DASWGM010000000
VERSION     DASWGM010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178376"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGA15_16145"
     CDS             complement(<1..426)
                     /locus_tag="VGA15_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HEX9589263.1"
                     /translation="MAESHGEAKGQKTGAHTEADGGHGGAFPPFDSSTFASQLVSLVI
                     AFVALYLIVSRIALPRVGSVIDARQNVIEGDLAEAQKLKDESDAALKAYENELAAARA
                     RAHAISAETREKLNAASDAERKRLEERLTHKLIEAEKTIA"
     gene            complement(487..717)
                     /locus_tag="VGA15_16150"
     CDS             complement(487..717)
                     /locus_tag="VGA15_16150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX9589264.1"
                     /translation="MEPAAAKLIGAGIACIGMGGAGVGVGVIFGNYLAAAVRNPSAAQ
                     GQFGNLIFGFAVTEALGIFSLLIALLLLFVPL"
     gene            complement(773..1528)
                     /locus_tag="VGA15_16155"
     CDS             complement(773..1528)
                     /locus_tag="VGA15_16155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX9589265.1"
                     /translation="MKIDPIHQFNIEPLFTIGHIGNQTIAFTNSSLYMFLSVAVISIL
                     MIGGVAGRQLVPGRFQSMAELTYEFVASTVRNTAGAEGMKFFPLIFSLFMFICVSNLV
                     GIIPYTFTVSSHIIVTAALALLVFFTVLIYGLYKNGLKFFKIFVPSGIPIYILPLVVF
                     IEIFSFFLRPISHSVRLFANMLAGHISLKVFAGFVAMLGTSLGAIGWLGGVMPLALTV
                     ALTALELLVAFLQAYVFAILTCIYLNDAIHPGH"
     gene            complement(1601..1969)
                     /locus_tag="VGA15_16160"
     CDS             complement(1601..1969)
                     /locus_tag="VGA15_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX9589266.1"
                     /translation="MADGTKDSGNGSRDKSPDEAALSARLGSLDHRLSEIRDNRKIRT
                     DQPGSGSGDGAARASAMARGFRLSSELIAGVVVGAVIGWGFDRLLSTSPFGLIVFFLL
                     GFVAGVVNVVRSAGVAPGRR"
     gene            2241..>2582
                     /locus_tag="VGA15_16165"
     CDS             2241..>2582
                     /locus_tag="VGA15_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX9589267.1"
                     /translation="MPRQVDYYFSLQSPWAYIGHKLFREVANTHGLKVNHKPVVLIDL
                     FSETGGLPLAKRHPVRQRYRMVELQRWRDKRGLNFHVQPANWPFNARLADGVVIAAVE
                     AGRDPDPFLRRA"
BASE COUNT          542 a          757 c          803 g          480 t
ORIGIN      
        1 tgcgatggtt ttttcggcct cgatgagctt gtgcgtcaaa cgctcttcca gcctcttgcg
       61 ctcggcatcg gatgccgcgt tcagcttttc gcgagtctcg gcgctgatcg cgtgcgcgcg
      121 ggcgcgcgcc gcggccaatt cgttttcata ggctttcagc gcggcgtcgg actcgtcctt
      181 cagcttctgc gcttcggcaa gatccccttc aatcacgttc tggcgtgcat cgatcacgct
      241 gccgacccgc ggcagcgcga tacgcgacac gatcaggtag agcgcgacga aggcgatgac
      301 cagcgacacc agctgcgaag cgaaggtgga ggaatcgaac ggaggaaatg ctccgccgtg
      361 accgccatcg gcttcggtgt gagcgccggt cttctggcct tttgcttcgc catgactctc
      421 agccatggag ttctcctgtt gctgtcatgc gcgtcccccg gaagcggcgg cgcgaagggg
      481 tgccgctcag agcggaacga acagcaggag caacgcgatc agcagcgaga agatgccgag
      541 cgcttcggtc acggcgaagc cgaagatcag gttgccgaac tggccctggg cggcggacgg
      601 attgcgcacc gcggcggcga ggtagttgcc gaagatgacg cccacgccaa cgcccgcacc
      661 gcccatgccg atgcacgcga tgcccgcgcc gataagtttt gctgctgccg gttccatttt
      721 tagctccttg aggaaagaaa gacgaagagt gggtgggaat tcccgggacc gtttagtgtc
      781 ccggatgaat ggcgtcgttg agatagatgc aggtgaggat cgcgaacacg taggcctgca
      841 ggaacgcgac cagcagttca agcgcggtca gcgcgacggt gagcgccagc ggcatcacgc
      901 cgccgagcca gccgatggcg ccgagcgagg tgccgagcat cgcgacgaag cccgcgaaca
      961 ccttcagcga gatgtggccg gccagcatgt tggcgaacag gcgcacgctg tgcgagatcg
     1021 gccgcaagaa gaacgagaag atttcgatga agacgaccag cggcaggatg tagatcggaa
     1081 tgcccgacgg tacgaaaatc ttgaagaact tgaggccgtt cttgtagagg ccgtagatca
     1141 gcacagtgaa gaagaccaag agcgccagcg ccgcggtgac gatgatgtgg cttgaaaccg
     1201 tgaacgtgta cggaatgatg cccacgaggt tcgaaacgca gatgaacatg aacagcgaga
     1261 agatcagcgg aaaaaacttc atgccttccg cgccggcggt gttgcgaacg gtgctggcga
     1321 cgaactcgta agtaagctcg gccatcgact ggaaacgccc gggaacgagc tgccgcccgg
     1381 cgacgccgcc gatcatcagg atcgagatca ccgccaccga caggaacatg tagagggacg
     1441 aattggtgaa ggcgatcgtt tgattgccga tatggccgat cgtgaacagc ggttcgatgt
     1501 tgaactggtg gatcgggtcg attttcatcc gcgcggctct tggtcctccg gctttcctgc
     1561 cggcaatcga atttcaaata ccaggacttc ccgtcagcgc tcagcgcctg cctggagcca
     1621 cgcccgcaga cctcaccaca ttgaccacgc cggcgacaaa gccgagcaga aaaaacacga
     1681 tgagaccgaa cggcgatgtc gacagcaagc ggtcgaatcc ccagccaatc accgctccga
     1741 caacaacgcc ggcgatcaac tccgaggaaa gccggaaacc gcgcgccatc gccgaggctc
     1801 tggcagctcc gtctccactt ccagatccgg gttggtcagt cctgattttg cggttgtccc
     1861 gaatttcgga caaacggtga tccagacttc cgagccttgc ggaaagcgca gcttcgtcag
     1921 gcgatttatc gcgacttcca tttccgctgt ctttcgtgcc gtccgccatg ctgtggagac
     1981 ccgaaacgat gccgcagttg atggaaatta cgccccgtca ccctcaaaag ccgcgcggac
     2041 catactgacc gcgtcaaatc aagtcaagat tgcgtcgtaa ccacttagtt cgttgatttg
     2101 attggaatat tcaggtgaaa tcgaacccgg gcggagccat cgcggcaccg caatagctgg
     2161 cttgagcggc gacgaccttg cacggttgcg gtcggtctgt tgggatgtcc ggaacatcca
     2221 aattgcgctc gggagcccgc atgccgcgtc aggtcgatta ctatttttcg cttcaatccc
     2281 cgtgggccta tatcgggcac aaactgtttc gggaagtcgc aaatacccac ggtctcaagg
     2341 tcaatcacaa gccggtggtg ctgatcgatc tgttctcgga gactggaggc ttgccgctgg
     2401 cgaagcgcca tccggtgcgg cagcgctatc ggatggtcga actgcagcgc tggcgcgaca
     2461 agcgtggcct gaattttcat gttcagccgg cgaattggcc gttcaacgcg cggctcgccg
     2521 acggcgttgt gatcgcggcg gtcgaggccg gtcgcgatcc cgatccattt ctgcggcggg
     2581 ca
//
LOCUS       DASWGM010000566         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2142766,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000566 DASWGM010000000
VERSION     DASWGM010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2142766"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGA15_16170"
     CDS             <1..220
                     /locus_tag="VGA15_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX9589268.1"
                     /translation="VGAFAYDLYKNRADMTADHIGIIAIGFVVSFVTAIIVVKTFLAY
                     VTRHGFTLFAWWRVIVGTLGLIALAMGR"
     gene            complement(281..1246)
                     /locus_tag="VGA15_16175"
     CDS             complement(281..1246)
                     /locus_tag="VGA15_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX9589269.1"
                     /translation="MASNLDTLVTVFGGSGFVGRSVVRALCKRDYRIRVAVRRPELAG
                     HLQPLGKVGQIHAVQANLRYPASVEAAMRDSHVAINLVGILTETGAQTFDAVQAAGAG
                     AVARAARAVGARMVHVSAIGADENSPSRYARAKAAGEKAVLSATPAATIMRPSVVFGP
                     EDQFTNRFAALARMSPMLPLIGGGLTKLQPVYVGDVATAVADAVDGKTKAGATYELGG
                     PEVLTMREIMEIILATIERHRMLVSLPFGLAKLQAQLLQFAPGPLKLTPDQVALLRVD
                     NVVSDAAKAAGLTLEGLGIAADSLEAIAPQYLWRFRAAGQFQRVI"
     gene            1385..1471
                     /locus_tag="VGA15_16180"
     tRNA            1385..1471
                     /locus_tag="VGA15_16180"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1419..1421,aa:Leu,seq:cag)
BASE COUNT          287 a          538 c          514 g          297 t
ORIGIN      
        1 ggtcggcgcg ttcgcctacg atctttacaa gaaccgcgcc gacatgacgg cggatcacat
       61 cggaatcatc gcaatcggtt tcgtggtgtc gttcgtcacc gcgatcatcg tggtgaaaac
      121 gtttttggct tacgtgaccc gccatggctt cacgctgttt gcgtggtggc gcgtgatcgt
      181 cggcacgctg gggctcattg cgctggcgat ggggcggtag gtcaagagca cgccgtcatc
      241 cctgcgtaat ggcttcgcca gtacgcaggg atgacgggcc ctaaattacg cgctgaaact
      301 gtccggcagc gcggaagcgc cagagatatt gcggcgctat tgcttccagc gaatccgccg
      361 cgattcccaa tccctccagc gtcagcccgg cggccttcgc ggcgtccgac acgacattgt
      421 cgacgcgcag cagtgccacc tgatccggcg tcagttttag cggacccggc gcgaactgca
      481 gcaactgcgc ctgcaatttt gcgaggccaa acggcaatga gaccagcatg cggtgacgct
      541 cgatggtcgc caggatgatt tccatgatct cgcgcatcgt cagcacttcc gggccgccga
      601 gctcgtaggt cgcgccggct ttcgttttgc catcaacggc gtcggcgacc gcggtcgcga
      661 cgtcgccgac atagaccggc tgcagttttg tcaaaccgcc gccgatcagc ggcagcatcg
      721 gcgacatcct tgcaagcgcc gcgaagcggt tagtgaactg gtcctcgggc ccgaacacga
      781 ccgaaggccg catgatggtg gctgcggggg tggctgacag caccgctttt tcgcccgccg
      841 ccttggcccg ggcgtagcgc gacggcgagt tctcgtcggc gccgatagcc gagacatgca
      901 ccatccgcgc cccgacggca cgcgcggccc tggccacggc gccggcgccc gcggcctgca
      961 ccgcatcgaa ggtctgcgcg ccggtttcgg tcaggatccc gaccagattg atggcgacgt
     1021 gggaatcgcg catcgcggcc tccaccgaag ccgggtagcg cagattggct tgcacggcgt
     1081 ggatctggcc gaccttgccg agtggctgca gatgaccggc cagttccggc cgccgcaccg
     1141 cgacccggat ccggtaatcg cgcttgcaga gcgcgcggac gacgcttcgg cccacgaacc
     1201 ccgatccgcc gaaaaccgtg accagcgtgt ccagattcga tgccatggcg cgcattccca
     1261 aaccgtctaa atgcattgaa gcaagggtgt tttagccgtt tcgcgatacg ccatcgggcc
     1321 tgcgttgtac agcggatttc aacaatttga caagcgcgcg accgatccgt actaaccggc
     1381 ccgggcccag gtggcggaat tggtagacgc gctggcttca ggtgccagtg gcttaacggc
     1441 cgtgaaggtt cgagtccttt cctgggcacc acttacgccc aagccccata acctgacatc
     1501 attccgatgc tctgacccgc accaaaagct tcagcgcctg ctcggcgcga cgcagcggct
     1561 cgaggctgcg gtaggttcgc gccagcaaca gcgcgccttc gagctggctc agcaccatgg
     1621 tcgcggcgag atccgc
//
LOCUS       DASWGM010000567         8341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1157238,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000567 DASWGM010000000
VERSION     DASWGM010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8341
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157238"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..1348
                     /locus_tag="VGA15_16185"
     CDS             350..1348
                     /locus_tag="VGA15_16185"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9589270.1"
                     /translation="MKITIVAFEGCMTSAVFGQADAFAVAAYISERRADSSWCGHDVR
                     LATPGGKPVRGYGGHLIEPDCSLADADDSHVVLIPPIFNDIEQTLAQEAGLVSWLASF
                     PANSKLLASTCTGAFLLAEAGILDGRRITTNPAFSVLFQQRYPGVRLALDERIIDDNM
                     VICAGATTAYLNLAIHVIDRLAGHDLAVSTAKALSIDRNPESQRPYFLFIAPKDHGDD
                     KVLRLQTWIEVHHRDPISIDDMVGAAGMSVRNLNRRFLTATGMAPRQYLRRVRIETAK
                     RLLEEQNVPVDRVAEQVGYGDTRAFIRAFGVLAGLSPGQYRQKFRALRRELPPIKG"
     gene            1438..2214
                     /locus_tag="VGA15_16190"
     CDS             1438..2214
                     /locus_tag="VGA15_16190"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9589271.1"
                     /translation="MPAIIHERSGHLAHIRLNRPDNGNRFTTEMFRTLAQMFTALDAD
                     PEIRCTLLTASGEDFSLGVDVPDVLPAWAAGKSPFEDSQVNPFGTTGPKRRKPLVTVV
                     HGQCFNAGLELVFASDICIAADNCRFAFHEMRFGVYPFGGGLFRFIRAAGWNAAMRYS
                     LTSEEFGADEARRMNVVALVRPSAEAEAAGLALAREICNAAPLAVQAAIAQAQTWADR
                     GDAAAFAHSVPDIIRLLNSRDAAEAMRAMAEGRAPAFSGQ"
     gene            complement(2203..3705)
                     /locus_tag="VGA15_16195"
     CDS             complement(2203..3705)
                     /locus_tag="VGA15_16195"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX9589272.1"
                     /translation="MADYDLAVIGGGLNGVSVARDAAGRGLRVILLEQGDLGAAASSA
                     SPRLIHGDLAMLERRAFFRVRAALAERDIWLQIAPHLVRPMRFAIPAHSDERPSWLLR
                     SGLLLYDRLASRSELPPSATVDVTHHPVGIALKRPFGTAFEYSDCVVDDSRLVIATAM
                     DAAERGAVIRTGARCVRADRLDIWRLVTKDRGHRQIITARALANATGAWTASVAETVL
                     RMPPPNLGTIQRGQIIVPRLFDTDHVYVFQNTDRRLIFASPYQRDFTLIGTVGHAFKG
                     DPAAVSMAASDVVYLCDAANRYFRERVEPSDVIRTVSGANVVDPADRRAARDGAMAFE
                     ARRGKAPLLTIFGGDVTTSRLRAERAVSRLTPFYPMSPRWTAKAPLPGGDFAWDRFDE
                     EVDGARERWRFLTEARAQRLVAAYGSRLAAILGDAKTGADLGPAFGPELTGAEVRYLM
                     ACEWARFPDDILWRRSKLGMTMPPGDREALAAFMAAAPRTSDARASLSLS"
     gene            complement(3782..5977)
                     /locus_tag="VGA15_16200"
     CDS             complement(3782..5977)
                     /locus_tag="VGA15_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589273.1"
                     /translation="MAEGDPSQPRWTLSGGLLIGVRWTGRTLKGLVLVLAAFGLVFLI
                     GVRWMGRILKGLVLVVLAAFVLVFLSNLLFFPSYRHIPSRALAADSASCRDALSPGWD
                     ALAAANNSEWTAIDQNSISARKLACSIQHHIIPARNLAPGQGDEAPKSLSYDLGFIEF
                     QEDGKPYALRKECDPGEECIRERSIPVRRQAEGQLEALVKQLKGNKSNYVVVFIHGWR
                     HDASIGDGDVANFRIYAAHAARFVADRAALDDAYKDTKVTAIYIGWRGARTDETWLSR
                     KFPLIGDAVGTASAVLTLFDRKPVSEAIAPSALSALRTIENTLGIDAYSELPRKMPNR
                     MIVFGHSLGGNMLMTALQDDLVKKVALHQPGSYMQPVLGDLVVLINPASEASKWTAVQ
                     RTLWRRLALVNGERRSGKDYEASQVFFRDDQRPFFISATSARNWPPGGRQQLDCSFGD
                     KVQNAENILVHQIADYDWATHDMFPAFKGDLRPLADRLELWGTQRDPNDECAQVRRSI
                     GDRIRHPLRTAAVFGSEFIRVLPFMQTDPEQTQTIGNLDPPRSPRGNLRTYGATMKPF
                     GTTHELRTSDRIWQHVARKTSVQDTNREIPLGYGEIMMPEASCPVAAGWLSRAKNEKI
                     QEQAKADPGPHRHYATNWDSYDLKVSTVANEGFPALRFVHGFADSGISPPTRPNDPFW
                     NLRAFDTALAEHDGYMLSSFICAMNQLALDDITTILPLTPVSVPTPDRP"
     gene            complement(6195..>8341)
                     /locus_tag="VGA15_16205"
     CDS             complement(6195..>8341)
                     /locus_tag="VGA15_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9589274.1"
                     /translation="PNGGSVATHEDCSEQRKLSRQLASTKQFLESMLDNVPVCVAAKS
                     IEDGRYIFANRAFERFSRFSRDHILGKRADEIFQAETAAGIVAADRSALNSSDGQFRN
                     EIVVQRGSKKRVLASTRVIVRDDNRQPEFLIALFDDVTDRQSLSQELEDTKKFLELVV
                     DNIPVSLIVERVSDGRYLLANRSAEAILNRRREDATGLTAADIFNPREAKLIIARDEA
                     AIKKRGLLTEEHPISTKDGLRLFLTRRMTVLDDSGEPQYLIKTHEDVTDRRQTESRMA
                     HMAYHDGLTDLPNRAAFLQALAQMIEACEGTNEEFAVLSVDLDGLKEINDVFGHAIGD
                     KLLIEVSRRIQTSARGGVVARLSGDEFGLIIDGKQPVAGKALAEQLAEVLAQEFPIDG
                     KSVRIGVTTGISIFPHNGADAASLLANAGAALFRAKAKSRGSISVYEPEMDQQIRDRR
                     VLHQDLSLAIKNGELSLHYQPQAASGQTVASSEVIGFEALARWLHPVRGFVPPGDFIP
                     LAEESGLIVEMGEWILREACREAASWSKPLQIAVNLSPAQFMHGDLVSLVHSILLETG
                     LTPGRLELEITEGVLIEDFDRGLALLRRLKALGVRISMDDFGSGYSSLSYLQAFPFDK
                     IKIDRAFVINLGRNPQSAAIVRAVIGLGHGLEMSIVAEGVETQEQLGFLADEGCDAVQ
                     GYFLGKPAPIGQYAALVGRSGGAAIELARKTG"
BASE COUNT         1566 a         2665 c         2578 g         1532 t
ORIGIN      
        1 aacttcttga ccaccgcgtc gatacgcagc agcggaacgt cggttgtata tcccagcggt
       61 tcgggtttcg gtagttcgtc agccatgatc ctcgccgccc atcatggccg cacgctagcg
      121 gcggatcggg cccggctcaa ccgtatcagc gtcgcaccgc cacaatattg tcggtgacgt
      181 tggtctcatc tctggtaagg ctcgacagat cggcaagact gccgagcgac gtcggccgtc
      241 ggccgcgagg cgggaagacg ttgacgcaac tcaaagaggc ggaagacgtc gacgcaactc
      301 gaaccggaaa cgccgtgctg cgggcaaccg gttcgtgtat tccaccccta tgaaaatcac
      361 gatcgtcgca ttcgaaggtt gcatgacctc ggccgtgttc ggtcaggcgg acgcttttgc
      421 cgttgccgcg tacatctccg agcggcgcgc cgattcaagc tggtgcggcc atgatgtccg
      481 gctggccacg ccgggcggga aacccgtgcg gggctatggc ggacacctga tcgagcccga
      541 ttgctcgctc gccgatgctg acgacagtca tgtcgtgctg atcccgccta tttttaacga
      601 catcgaacag accctggcgc aggaagccgg cctcgtctcc tggttggcgt catttccggc
      661 aaattcaaag ctgctagcgt ccacctgcac cggcgcgttc cttctcgccg aagccggcat
      721 cctcgacggc cggcgcatta ccaccaatcc cgcctttagc gtgcttttcc aacagcgcta
      781 tccgggcgtg cgcctggcat tggacgaacg tatcatcgac gacaacatgg tcatctgcgc
      841 aggcgcaacc actgcatatc tcaacctcgc gatccatgtc atcgatcggc tggccggcca
      901 tgatcttgcg gtatcgacgg caaaggcgct gtcgatcgac cgcaatccgg aatcgcagcg
      961 accctatttc ctgttcatcg cccccaagga tcacggcgac gacaaggtgc tgcgacttca
     1021 gacatggatc gaagtccatc atcgggaccc gatcagcatt gacgatatgg tgggcgccgc
     1081 cggcatgagc gtgcgcaatt tgaaccgccg ctttcttacc gccaccggta tggcgccgcg
     1141 gcagtacctt cgcagggtcc gcatcgaaac cgccaagcgg ctgcttgagg aacagaacgt
     1201 gcccgtcgac cgcgtggccg aacaggtcgg ttacggcgac acccgcgcct tcatccgggc
     1261 attcggggtt ctcgccgggc tttcgcccgg ccagtaccgc caaaaattcc gagcgctgag
     1321 gcgcgaactg ccgcccatca agggctgaaa cggcaacctg aacggccggg cccgccatgg
     1381 tcgcccgcag cccgatcggg gacattgccg ttacgtccag cgcactggag ccccccgatg
     1441 cccgcaatca tccatgaacg atccggacac ctggcccaca tcagattgaa ccgtcccgac
     1501 aacggcaatc ggttcacgac ggagatgttt cgcacgcttg ctcagatgtt tacagcgctg
     1561 gacgccgacc ccgagattcg ctgcaccctg ctcaccgcaa gcggcgaaga tttttcactc
     1621 ggcgtcgatg tgccggatgt gctgccggcc tgggccgcag gcaaaagccc cttcgaggac
     1681 agccaggtca atccgttcgg cacgaccggt cccaaacgcc gcaagccact cgtcaccgtc
     1741 gtccacggcc agtgtttcaa tgcagggctc gaactggtgt tcgcttcgga tatctgtatc
     1801 gccgccgaca attgccgttt tgcgtttcac gaaatgcgct tcggagtcta tccgttcggc
     1861 ggcgggcttt tccgttttat tcgggcggcg ggctggaatg cggcgatgcg ctattccttg
     1921 acgtcggagg aattcggcgc cgacgaggcg cgtcgcatga atgtggtagc gctggtgcgg
     1981 ccctcagccg aggccgaagc cgctggtctc gcgctcgcgc gggaaatctg caacgccgct
     2041 ccccttgccg tgcaggccgc catcgcccag gcccagacat gggcggatcg cggggacgca
     2101 gccgccttcg cgcattcggt accggatatc atccgattgc tgaactcacg cgatgcggct
     2161 gaggcgatgc gggcgatggc cgaggggaga gctccggcgt tttcaggaca gtgagagcga
     2221 agcgcgagca tccgaggttc gaggcgcggc cgccatgaac gccgccagcg cttcgcgatc
     2281 tccgggcggc atggtcatgc cgagttttga gcgccgccac aggatgtcgt ccgggaaacg
     2341 cgcccattcg catgccatga gatagcgcac ctccgcgccc gtcagttccg ggccgaaggc
     2401 gggaccgagg tcggcgccgg ttttggcgtc gccgaggatg gccgcaaggc gcgagccata
     2461 ggcggcgacc aggcgctgcg cccgagcctc ggtcagaaac cgccaccgct cgcgcgcacc
     2521 atcaacctct tcgtcgaacc gatcccacgc aaaatcgcct cccggcaggg gcgccttcgc
     2581 ggtccagcgc ggcgacatcg gatagaacgg cgtgagcctg gagaccgccc gctcggcgcg
     2641 cagccgcgag gtcgtgacgt cgccgccaaa gatcgtcagc agcggcgcct tgccgcgcct
     2701 cgcctcgaac gccatcgcgc catcccgcgc ggcgcgccgg tctgcggggt ccaccacgtt
     2761 ggcgccggaa acggtcctga tcacgtcgga tggttcgacc cgctcgcgaa aatagcggtt
     2821 ggccgcgtcg caaagataca cgacatcgct tgcggccatg gatacggcgg ccggatcgcc
     2881 cttgaacgcg tgcccgacgg tgccgatcag cgtgaaatcg cgctggtagg gactggcgaa
     2941 aatcagccgc cggtccgtgt tctggaaaac atagacgtgg tcggtatcga acagccgcgg
     3001 gacgataatc tggcccctct ggatcgtgcc gaggttcggc ggcggcatcc gcaataccgt
     3061 ctccgcaacc gacgcggtcc aggcgccggt agcgttggcc agcgcccttg ccgtgatgat
     3121 ttgacgatgg ccgcgatctt tcgtcaccag ccgccagatg tcgagccggt cggcgcgaac
     3181 gcagcgcgcg ccggtgcgga tcacggcgcc ccgctcggcg gcatccatgg ccgtggcgat
     3241 caccagccgc gaatcgtcca ccacgcagtc ggaatattca aaggcggtgc cgaatggccg
     3301 cttcagcgcg atgccgaccg gatggtgcgt gacatcgacc gtggctgacg gcggcaactc
     3361 gctgcgcgag gccagccggt cgtagagcaa taaccccgac cgcagcagcc atgatgggcg
     3421 ctcgtcggaa tgcgcgggga tcgcaaagcg catcgggcgg accagatgcg gcgcgatctg
     3481 gagccagatg tcacgctcgg ccagcgcggc acggacgcgg aaaaacgccc tgcgctccag
     3541 catcgccaga tcgccgtgga tcagccgtgg cgaggccgag gacgcggcgg cgccgaggtc
     3601 gccctgctcc agcaggatca cccgcaggcc gcgtcccgcg gcgtcgcgcg caacgctgac
     3661 gccgttcaag ccaccgccaa tgaccgcaag atcgtagtcc gccacgtctg agctccaact
     3721 ccgtccacca tgacaagccg tgcgggcaaa actaagccgc ggcgcacagg aagcaaaaat
     3781 tttatggacg atcgggagtc ggaacggaaa ctggagttag cggaagaatg gtggtgatat
     3841 cgtcaagtgc gagttgattc atggcgcaga taaatgacga aagcatgtac ccatcgtgct
     3901 ctgcgagcgc cgtgtcgaac gcccgcaaat tccagaacgg atcgttggga cgagttggcg
     3961 gagaaatccc gctatcggca aagccgtgga cgaagcgcag ggccgggaag ccttcgttgg
     4021 cgaccgtgct caccttcaga tcataactat cccaattggt tgcataatgc cggtgcgggc
     4081 ccgggtctgc tttcgcctgc tcctggatct tttcgttctt ggcgcgcgac agccagcctg
     4141 ccgcgaccgg gcatgatgcc tcgggcatca tgatctcccc gtagccaagc ggtatttccc
     4201 tgtttgtgtc ttgcaccgac gttttgcggg cgacgtgctg ccagattctg tccgatgtgc
     4261 gcaattcatg ggtggtgccg aacggcttca tggtcgcacc ataggtccgc agattgcctc
     4321 gcggcgaacg tggcgggtcg aggttgccga tggtctgggt ctgctccggg tcggtctgca
     4381 tgaacggcaa cacgcggatg aattcactgc caaagacagc cgcggtacgc aacggatgac
     4441 gtatcctatc accgatcgac ctgcgaacct gagcgcattc gtcgttggga tcacgttgcg
     4501 tgccccataa ttcgagccgg tcggccaagg gacggagatc gcccttgaac gccggaaaca
     4561 tgtcgtgcgt cgcccaatcg taatccgcga tctggtgaac cagaatattt tcggcgtttt
     4621 gcactttgtc gccgaagctg cagtccagct gctgcctgcc cccgggcggc cagttgcgcg
     4681 ccgacgtcgc cgaaatgaag aagggacgct gatcgtcacg gaaaaacacc tgcgacgctt
     4741 cataatcctt gccggaacgc cgctcgccgt tgactaaggc aagcctgcgc cacagcgtcc
     4801 gctgaacggc agtccatttg gaagcctccg aagccggatt gatcagcacg accagatcac
     4861 cgagcacggg ctgcatgtag gaaccgggtt ggtgcagggc gactttcttg acgaggtcat
     4921 cctgcagggc agtcatcagc atgttgccac ccagagaatg gccgaaaacg atcattctgt
     4981 tcggcatttt ccgcgggagt tcgctatatg catcgatccc cagggtgttt tcgatcgtcc
     5041 gcagcgccga caacgccgag ggggcaatcg cctcgctgac cggcttgcga tcgaacaacg
     5101 tcagaaccgc ggaagccgtg ccgacggcat ccccgatcaa gggaaacttt cgggatagcc
     5161 aggtctcgtc ggtccgcgca cccctccacc cgatgtagat cgcggttacc tttgtatctt
     5221 tgtacgcatc gtccaaggcg gcacgatctg caacaaaacg cgccgcatgg gcagcataga
     5281 ttcggaaatt ggcaacgtcg ccatcgccga tgcttgcgtc gtgccgccac ccgtggatga
     5341 agaccacgac gtagttcgat ttgtttccct ttagttgctt gaccagcgcc tcgagttgac
     5401 cctctgcctg acggcggacc ggtatcgacc gttcacggat acattcctcg ccgggatcac
     5461 attcctttcg caaggcatag ggtttgccgt cctcctggaa ttcaataaat ccgagatcgt
     5521 aacttagcga tttcggcgct tcgtcgcctt gcccgggagc caggttgcga gctggtatga
     5581 tgtggtgctg gatcgaacac gcaagcttgc gcgcggaaat gctgttttgg tcgattgccg
     5641 tccactcgct gttgttcgca gccgcaaggg cgtcccaacc cggggaaagt gcatcccggc
     5701 aggacgcgga atcagcggcg agagcgcggg agggaatgtg ccggtagctc ggaaaaaaca
     5761 gcaggttgct caggaagacg agaacgaacg cagcgagcac caccaggacg agccctttga
     5821 gtatcctgcc catccatctg accccgatca ggaagacgag accgaacgct gcaagcacca
     5881 ggacgagccc tttgagtgtc ctgcccgtcc atctgacccc gataagcagc cccccagaca
     5941 gcgtccagcg cggttgagac ggatctccct cggccatttg cgttcccccc gagattcgcc
     6001 gcgtcagcac gatcttgcac gttgcatcgg gctgcgcttg gcaaagcaat gcgccggaca
     6061 aattcgtcac aacaacggcg tcggagaaat tttcgcgcga tccgacgggc cggcgatcat
     6121 cataagatgt attgtattgt gcaaccagtc agattgtcga acagtcgcga gctataagcg
     6181 gcctgcgcgc cgcgctagcc ggtcttgcgc gcgagctcta tcgcagcgcc gccgctacgg
     6241 cccaccagcg ccgcgtactg tccgatcggc gcgggcttgc cgaggaaata gccctgtacc
     6301 gcgtcacagc cctcgtcggc gaggaacccg agttgctcct gggtttccac gccctcggcg
     6361 acgatcgaca tttcgagacc gtggccgagg ccgatgacgg cccggacgat cgccgccgat
     6421 tgcggatttc gtccaagatt gatgacgaag gcgcggtcga tcttgatttt gtcgaacgga
     6481 aacgcctgca gatagctcaa tgaggaatag ccgctgccga aatcatccat cgagatacga
     6541 acgccgagcg ccttcagccg ccgcagcagc gccaggccgc gatcgaagtc ctcgatcagc
     6601 acgccttcgg tgatttccag ctcgagccgg cccggcgtga ggccggtttc gagcagtatc
     6661 gagtgcacga ggctgaccag atcgccatgc atgaactgcg ccggtgacag gttgaccgcg
     6721 atctgcagcg gcttcgacca cgacgcggcc tcccggcagg cttcgcgcaa gatccattca
     6781 cccatttcga cgatcaagcc gctttcttcc gcgagcggga tgaagtcgcc cggtggaacg
     6841 aagccgcgga ccggatggag ccaccgcgcc agcgcctcga agccaatgac ttcgctggag
     6901 gcaaccgtct ggcccgaggc cgcctgcggc tgataatgca gcgacagctc gccgttcttg
     6961 atcgcgagcg agagatcctg gtgcagcacg cggcgatcgc gaatctgctg gtccatctcg
     7021 ggttcataga cgctgatcga gccgcgcgat ttcgccttgg cgcggaacaa tgccgcgccg
     7081 gcgttggcaa gcagcgatgc ggcgtcggcg ccgttgtgcg ggaatatcga gatgccggtg
     7141 gtgacgccga tgcggaccga tttgccgtcg atcgggaact cctgcgccag cacttcggcg
     7201 agctgttcgg ccagcgcctt cccggcgacg ggttgcttgc cgtcgatgat caatccgaac
     7261 tcgtcgcctg agagccgggc cacgacgccg ccgcgcgccg aggtctggat gcggcgcgac
     7321 acttcgatca gcagcttgtc gccgatcgca tggccgaaca cgtcgttgat ttccttcagc
     7381 ccgtccagat ccacggaaag taccgcgaac tcctcattgg tgccttcgca ggcctcgatc
     7441 atctgcgcca gtgcctgcag gaacgccgcg cggttcggca gatccgtcag gccgtcatga
     7501 tacgccatat gagccatgcg cgattcggtc tggcggcggt cggtgacgtc ctcgtgggtc
     7561 ttgatcagat attgcggctc gccggaatcg tcgagcaccg tcatacgccg cgtcaggaac
     7621 agccgcagcc cgtccttggt gctgatcggg tgctcttccg tgagcaggcc gcgcttcttg
     7681 atcgccgcct cgtcgcgcgc gatgatcagc tttgcttctc tcggattgaa gatatccgca
     7741 gccgtcaatc cggtggcatc ctcgcgacga cggttgagga tggcttcggc gctgcgattg
     7801 gcgagaaggt accgcccgtc gctgacgcgc tcgacgatca gcgagaccgg gatgttatcg
     7861 accaccagct cgagaaactt cttggtatct tcgagctcct gggaaagcga ctggcggtcg
     7921 gtgacgtcgt cgaacagcgc gatcaggaat tccggctgcc tgttgtcgtc gcgcacgatc
     7981 acgcgggtac tggccagaac gcgcttcttc gaaccgcgct ggaccacgat ttcattccgg
     8041 aactggccat cggacgagtt gagcgccgac cggtctgccg caacgatccc tgccgcggtc
     8101 tcggcctgga aaatctcgtc ggcacgcttg ccgagaatgt gatcgcggga aaagcgcgag
     8161 aaccgctcga acgcccggtt ggcaaaaata taacggccgt cctcgatgct tttggcggcc
     8221 acgcataccg gaacgttgtc gagcatcgat tccaggaact gcttggtcga ggccaattgt
     8281 cgcgacagct tgcgctgctc gctgcaatcc tcgtgcgtcg ccaccgaacc gccgtttggc
     8341 a
//
LOCUS       DASWGM010000568         4304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_593821,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000568 DASWGM010000000
VERSION     DASWGM010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593821"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..1904)
                     /locus_tag="VGA15_16210"
     CDS             complement(357..1904)
                     /locus_tag="VGA15_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9589275.1"
                     /translation="MTETSSLFLGIISGQRRRTHADVADRAARIAGGLQKLCVKPGDS
                     VCILMRNDIAFLEAAYAAMRLGAYAVPVNWHFKPEEINYVLQDSGTPVLIGHADMLPP
                     LRSAIPAGVTVLSAPTPPEILRSYKIDPDHLATPDFAPDLESWLERQKPYDGPTLPQP
                     ANMIYTSGTTGHPKGVRRNAPTPEQTIASERLRALIYGLKPGVRALLPGPLYHSAPNS
                     FGLRSGRLGGALVLMPRFDPEELLRLIEAERIDTIFMVPTMFIRLLKLPEAVRGKYDT
                     SSLRHVVHAAAPCPAEVKRAMIAWWGPVISEYYGSTESSALTFATSDDALRKPGTVGK
                     ILDGVELRFLGEDGEPLPQGEIGEIYSRVAGNPDFTYHNKPERRAEIERGGFITSGDV
                     GYIDEAGYVFICDRKRDMVISGGVNIYPAEIEAALHAVPGVHDCTVFGIPDDEFGEAL
                     MAVVEPQAGVTLDVADIRAQLKTSLAGYKVPKHLEILKNLPREDSGKIFKRRLRDPYW
                     ERAGRRI"
     gene            complement(2077..2889)
                     /locus_tag="VGA15_16215"
     CDS             complement(2077..2889)
                     /locus_tag="VGA15_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9589276.1"
                     /translation="MDDRVKVTISEGVADVRLVRADKMNALDAAMFDALVATCDRLSR
                     EKGLRVVVLSGEGRAFCAGLDMGRFAAMKEKGGNGVAGGENRDLSVRTHGLANFPQAA
                     VWGWRQLPVPVIAAIQGVAFGGGFQLALGADLRFLAPDARMSIMEIKWGLVPDMAGTP
                     ILASLVRDDILRELTYTGRIFSAQEALSYGLATRICDDPRATAFEVARDIAGKSPEAI
                     RAAKRMLNNLSVDPGPALLAESVEQMKLMGGPNQLEAVRANIDKRAPRFADV"
     gene            2999..3871
                     /locus_tag="VGA15_16220"
     CDS             2999..3871
                     /locus_tag="VGA15_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589277.1"
                     /translation="MFKQQLLAGRRILVTGGGTGLGKSMAARFLELGAEVHICGRRKG
                     VCDETATELMDLYGGRVTSHGVDIRNPLAVDEMIGTIFTEGPLTDLINNAAGNFVSRT
                     QDLTPRGFDAIANIVMHGTFYVTHAVGRRWIAAKQPGNVVSITVTWVRNGSPYVVPSA
                     MSKSAIHAMTMSLAMEWGRYGIRLNTIAPGEIPTEGMSKRLNPGEEAGARTIARNPMG
                     RVGKMEELQNLAVFLISGGCDWINGETIAMDGGQALATGGNFYELRDWSDADWIQARD
                     QIKAQNEKDRTARG"
     gene            complement(4063..>4304)
                     /locus_tag="VGA15_16225"
     CDS             complement(4063..>4304)
                     /locus_tag="VGA15_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9589278.1"
                     /translation="VEHTLSDRVSMCNFWANRLCCEAADRAMQVHGGMGYSRHKPFEH
                     IYRHHRRYRITEGSEEIQKRKVAGFLFGYMGEGKH"
BASE COUNT          828 a         1359 c         1329 g          788 t
ORIGIN      
        1 cgcagccgcg cgtccgcttc gatggcggcg atatcctcgc cccggttgga gcgcacctca
       61 gcatcggaag gcatgacctg ggaagacatg gctggctact ccagaaactt gcccggcttc
      121 ccttgagtcg caatttttca agcctggcgc aagtccaaga aaagggcgat catatctagt
      181 cgtccccgcg aaaagcgggg acccataacc cctaccgttt attattgacc gaatagcggc
      241 tccagccgct ctttgtaaaa acgatgcggc cggggttatg gatcccggcg cgcgcggagc
      301 ctgtcatcgg gcggcgcctc gcgccgaccc gttggcttgg ctgggacgac gacgttctaa
      361 atccttcgcc ccgctcgctc ccaataggga tcgcgcaagc ggcgcttgaa aatcttgccg
      421 gaatcctcgc gcggcagatt tttgaggatt tcgaggtgct tcggcacctt gtagcccgcc
      481 agcgaggttt tcagttgcgc gcggatgtca gcgacatcca gcgtcacccc ggcttgcggt
      541 tccaccaccg ccatcagcgc ctcgccgaat tcgtcgtcgg gaatcccgaa cacggtgcaa
      601 tcgtgaacgc ctggaaccgc atgcagcgcg gcctcgattt cggcgggata gatgttgacg
      661 ccgcctgaaa tcaccatgtc gcgcttacgg tcgcagatga agacgtaacc cgcctcatcg
      721 atgtaaccaa catcgccgga agtgataaaa ccgccacgct cgatctcggc gcgcctctcc
      781 ggcttgttgt gataggtgaa atccgggttg cccgcgacgc gcgaatagat ctcgccgatc
      841 tccccttgcg gcagcggctc gccgtcctcg ccaagaaagc gcagctcgac gccatcgagg
      901 atcttgccga cggtgcctgg tttcctcagc gcgtcgtcgg aagtggcgaa agtcagcgcg
      961 ctggattcgg tgctgccata atattcagag atcaccggtc cccaccacgc gatcatcgcg
     1021 cgcttgacct cggcggggca cggtgccgcg gcatgaacga catggcgcag cgacgaggtg
     1081 tcgtattttc ctcgcactgc ctccggcagc ttgagcagcc ggatgaacat ggtcggcacc
     1141 atgaaaatgg tgtcgatcct ctcggcctcg atcagccgca gcaattcctc gggatcgaac
     1201 cgcggcatca gcaccagcgc gccgccgaga cgccccgagc gcaagccgaa tgaattcggc
     1261 gcggaatggt acagcggccc cggcagcagc gcccgcacgc cgggcttcag gccatagatc
     1321 agtgcgcgca gccgctccga cgcgatggtt tgctccgggg tcggcgcgtt gcgtcgcacc
     1381 cccttgggat gtcccgtggt gccggaagtg tagatcatgt tcgctggctg cggcagcgtt
     1441 gggccgtcgt acggcttttg ccgttcgagc caggattcaa gatcgggggc gaaatcgggg
     1501 gtggcgagat gatcgggatc gatcttgtaa gacctgagaa tttcgggcgg cgtcggggcg
     1561 ctcagcacgg tgacgccggc cggaatggca ctgcgtaacg ggggcagcat gtcggcatgg
     1621 ccgatcagga ccggtgttcc ggaatcctgg aggacgtagt tgatctcctc cggcttgaag
     1681 tgccagttga caggcactgc gtaggcgccg agccgcatcg cggcataggc ggcctcgagg
     1741 aaggcgatat cgttgcgcat caggatgcag acgctgtcgc ccggcttgac gcaaagcttc
     1801 tgcaggccgc cggcgatacg ggcggcgcga tcggcgacgt cggcgtgggt tcgcctgcgc
     1861 tgaccgctga tgatgccgag gaaaagcgag gatgtttcgg tcattgtttt cttcttgaat
     1921 cttgaggagc cgtcatcctg aggagcgcgc tcttcgcgcg cgtctcgaag gatgagccac
     1981 gcggactgcc agcatccttc gaggctcgcc gaagacggct cgcacctcca gcgataacgg
     2041 cgcagccgtt acgcggggat gacgttcgaa tgttattcaa acgtcagcaa agcgcggcgc
     2101 gcgcttgtcg atattggcgc ggaccgcctc gagctgattg ggcccgccca tcagtttcat
     2161 ctgctcgacg gattcggcta acagcgccgg gccgggatcg acggacagat tgttcagcat
     2221 gcgcttggcg gcgcggatcg cctcggggct cttgccggcg atgtcgcggg caacctcaaa
     2281 ggccgtggcg cgcgggtcgt cgcagatgcg cgttgcgagg ccgtagctca gggcttcctg
     2341 cgccgagaaa atccgcccgg tataggtcag ctcccgcaaa atatcgtcgc ggacgaggct
     2401 cgccaggata ggcgttcccg ccatatccgg caccagcccc catttgattt ccatgatcga
     2461 catccgcgca tcgggcgcga gaaaccgcaa gtcggcgccg agcgcgagct gaaagccgcc
     2521 gccgaacgcc acgccctgga tcgcggcaat cactggaacc ggcagttgcc gccatcccca
     2581 caccgcagct tgcgggaaat tggccaggcc atgggtgcgg acgctcaaat cgcggttttc
     2641 gcccccagct accccgttac cgcccttctc cttcatcgcc gcaaagcggc ccatgtcgag
     2701 gccggcgcaa aaagcccgcc cctcgccgga taacaccacc acccgcagcc ctttttcgcg
     2761 ggagagccgg tcgcaggttg cgacgagggc atcgaacatc gcggcatcga gcgcattcat
     2821 tttgtccgcc cgcaccagac ggacatcggc gacgccctcc gaaatcgtaa ccttgacgcg
     2881 atcatccatg agagtttctc ccctgctctt ctttgttgtt gccactttac agaaaggcgt
     2941 tgtccggatt tagtcaatca accaattaac agacaaatct ccagcgcgga aacaacagat
     3001 gttcaagcaa cagcttcttg ccgggcggcg cattctggtc accggcggcg gaaccgggct
     3061 cggcaagtcg atggccgcgc gcttccttga actcggcgcc gaggttcata tttgcggccg
     3121 ccgcaaaggc gtctgcgacg aaaccgcgac tgaactgatg gatctgtatg gcgggagggt
     3181 gacgagccac ggcgtcgaca tcagaaaccc gctcgcggtc gatgagatga tcggcaccat
     3241 cttcaccgaa ggtcccctta ccgacctcat caacaacgcc gccggcaatt tcgtctcgcg
     3301 aacccaggac ctgacgccgc gcggcttcga tgcgatcgcc aacatcgtca tgcacggcac
     3361 gttctacgtg acgcacgcgg tgggccgtcg ctggatcgcc gccaaacaac ccggcaatgt
     3421 ggtgtcgatc accgtgacat gggtccgcaa cggcagcccc tatgtggtgc cgtcggcgat
     3481 gagcaagtcg gcgattcatg ccatgaccat gtcgctggca atggaatggg gacgctacgg
     3541 catccggctc aacaccatcg cgcctggcga aattccgacc gagggcatga gcaagcgcct
     3601 caaccccggt gaagaagccg gcgcgcgcac catcgccagg aatccgatgg gccgcgtcgg
     3661 caagatggaa gaactgcaga atttggcggt gttcctgatt tcaggcggct gcgactggat
     3721 caacggcgag accatcgcca tggacggcgg ccaggcgctg gcaacgggcg gaaacttcta
     3781 tgaattgcgc gactggtccg acgccgactg gatccaggcg cgcgaccaga tcaaggcgca
     3841 gaacgaaaaa gacagaactg caaggggatg agtttgtttc gtcgtcccgg cgaacgccgg
     3901 gatccatacg ccgtgccgtc gcgatttcac gctggagcgg atgccttctg caacaatcaa
     3961 cgtcgggggt tatgggtccc ggccttcgcc gggacgacgc actcattgga gcgacgtagt
     4021 gcatccaatg gaacaacgcg gcgcatccgc accaacaccc actcaatgct tcccttcccc
     4081 catatagccg aacaaaaatc ccgcgacctt gcgcttctgg atttcctcgc tgccttcggt
     4141 gatgcggtag cggcggtgat ggcggtagat gtgttcgaac ggcttgtggc gtgaatagcc
     4201 catgccgcca tgcacctgca tggcgcggtc ggcggcttcg cagcacaacc ggttggccca
     4261 gaaattacac atcgagaccc ggtcggacag cgtatgctcg acct
//
LOCUS       DASWGM010000569         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_458083,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000569 DASWGM010000000
VERSION     DASWGM010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458083"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VGA15_16230"
     CDS             complement(<1..893)
                     /locus_tag="VGA15_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9589279.1"
                     /translation="MAGYGVAAVAAVLILYPVFFLLQAALDVGDPQVRPPTAYGFDNF
                     NGIFQYPQILLNTLTVSAAATVMALVFGFVMAWILTRTNVPGRHLFEQLMAVPYYLTP
                     LLGALAWSLLGSPESGFINQIWRALGGNGHLIDINTAYGIAWVMALFEGSVAFVMIAA
                     VMKSMDPALEEASQIMGASRIRTMLRITLPLVVPGVLGAAIFVFAEMLGSFAAALVLG
                     LPSRYYVITTAIYQLVQQYPPKIQIAAAMGVSLFAVMFFMLFLYRRIVMTGSYVTITG
                     KAFRPRVADVGPLRYVLLGVCL"
     gene            complement(941..>2062)
                     /locus_tag="VGA15_16235"
     CDS             complement(941..>2062)
                     /locus_tag="VGA15_16235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9589280.1"
                     /translation="GESELADRKKGYVTRRTLLKAGAAMTAMPVLGIAAPSVLAQGPL
                     KSPTRVHDFMTNADVAKAEQEGQLVFYCHENEPGTAGIMEGFRKDFPKINTSYVRAQT
                     GALYNKILSERSAGRFDVDVIQLSDLAPAVDFQKKGGYEHYVSPETPAYKADHLSSPA
                     GSFFWTGVDPAGIAYNSTKVSTADAPKSWQDILNPRWKGSISCKISASGLQFVQWYTL
                     RKLYGAGFWKEFARQNPHAFDSRVQLFDRLAKGDDMITAIGEYPAYILFKDKGAKVEF
                     VAPADGLPATPLIVGAVSKAPHPEAAKLFVDWAMSKRGQAWYQTNPNLYYGSVRTDAP
                     PMPTGVRLGDLKLLYPADWDDYAANRDVFAKEWNGMLGL"
BASE COUNT          423 a          619 c          641 g          379 t
ORIGIN      
        1 aggcagacgc cgagcaacac gtagcgcagc gggccgacgt cggccactcg cggccggaac
       61 gccttgccgg tgatggtcac gtaactgccg gtcatcacga tgcgccggta caggaacagc
      121 atgaagaaca tcacggcaaa cagcgacacg cccatggcag ccgcgatctg aatcttcggc
      181 ggatattgct ggaccagctg gtagatcgcc gtggtgatga cataatagcg gctgggcagg
      241 ccgaggacga gcgcggcggc gaaggagcca agcatttcgg cgaagacaaa gatggcggcg
      301 ccgaggacgc cggggacgac caacggcagg gtgatacgca gcatggtgcg gatgcggctc
      361 gcccccatga tctgcgatgc ctcctcaagc gcggggtcca tcgacttcat aaccgcggca
      421 atcatgacga atgcaacgga gccctcgaac agcgccataa cccaggcgat gccgtacgca
      481 gtattgatat cgatgagatg gccgtttccg ccgagcgcac gccagatctg gttgatgaag
      541 ccgctttctg gcgagccgag caggctccag gccagcgcgc cgagcaacgg cgtcagataa
      601 tagggcaccg ccattagttg ctcgaacaaa tggcggccgg gcacgttggt gcgtgtcagg
      661 atccacgcca tcacgaaacc gaacaccagc gccatcaccg ttgcggcagc tgacactgtc
      721 agtgtgttga gcaggatctg cgggtactgg aatatgccgt tgaagttgtc gaatccgtac
      781 gcggtcggcg gccgcacctg tgggtcgccg acatcgagcg ctgcctgcaa cagaaagaac
      841 accgggtaca ggatcagaac cgcggcgacg gccgcaaccc catacccagc cattttctgc
      901 gcggagatta gcgatctcgc ggattgcacg gccgctccgc tcacaggccg agcatgccgt
      961 tccattcctt ggcgaagaca tcgcggttgg ccgcgtagtc gtcccagtcg gcggggtaaa
     1021 gcagcttgag atcacccagc cgcactccgg tcggcattgg cggcgcgtcg gtgcgcaccg
     1081 acccgtaata gagatttggg ttggtctgat accaggcctg gccacgcttc gacatcgccc
     1141 agtcgacgaa tagcttggcc gcttccggat gtggcgcctt gctgacggcg ccgacaatca
     1201 aaggcgtagc cggcaatccg tcggcgggcg cgacgaattc aaccttggca cctttgtcct
     1261 tgaataggat gtaggcggga tattccccga tcgcggtgat catgtcgtca cccttggcga
     1321 gccggtcgaa caactggacg cgggaatcga aggcgtgcgg attctgcctg gcaaactctt
     1381 tccagaagcc cgccccatac agcttgcgca gcgtgtacca ctgtacgaac tgcaatccgg
     1441 aggcggaaat cttgcagctg atcgatccct tccaccgagg attgagaatg tcctgccagc
     1501 tcttcggcgc gtccgcggtg gagaccttgg ttgagttgta agcgataccg gcggggtcga
     1561 caccggtcca gaaaaacgaa ccagccggcg aactcagatg gtccgccttg taagccggag
     1621 tctcggggga cacgtagtgt tcatagccgc ccttcttctg gaaatcgaca gcgggcgcca
     1681 ggtcggatag ttggatgacg tcaacgtcga aacggccggc ggagcgttct gacaggatct
     1741 tgttgtaaag cgcgccggtc tgcgctcgca catagctggt gttgatcttc gggaaatcct
     1801 tgcgaaatcc ctccatgatg ccagcggtgc cgggctcatt ctcgtgacaa tagaaaacga
     1861 gctggccttc ctgctcggcc ttcgccacgt cggcgttagt catgaagtcg tgaactcttg
     1921 tcggtgattt caacggaccc tgggccagta cgctgggtgc cgcaatcccg aggacaggca
     1981 tcgcggtcat cgcggcgcca gccttcagca gcgtgcgtcg cgtcacatag ccttttttcc
     2041 tgtcggccaa ttcactctcc cc
//
LOCUS       DASWGM010000570         7220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1287260,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000570 DASWGM010000000
VERSION     DASWGM010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287260"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VGA15_16240"
     CDS             complement(<1..916)
                     /locus_tag="VGA15_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX9589281.1"
                     /translation="MALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSAFVEEELE
                     RNPLLERASDGTEPPVAGEPTPERAEFSDRDDTGSAYGDDVAAERNEMAHGSTGEGFE
                     PAQEEWMSSDLGSRAEIEQTLDTGLDNVFSEEPAEAAARTAQDAAPTAYTEWGGGASN
                     DDSYNLEAFVAAEITLGSHLAEQLAVAFAAPAQRMIGQYLIDLVDEAGYVPPDLGQAA
                     ERLGASQKDVEAVLAVLQKFDPPGVCARSLSECLAIQLRELNRYDPAMQALVEHLDLL
                     AKRDIAALRKFCGVEDEDITDMIGEIRRL"
     gene            complement(1140..2132)
                     /gene="lptB"
                     /locus_tag="VGA15_16245"
     CDS             complement(1140..2132)
                     /gene="lptB"
                     /locus_tag="VGA15_16245"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX9589282.1"
                     /translation="MVDLLGMFRRRPAKRNPPGFARTRGDITSLEDAMEMLQQNQVRD
                     APPLARDGSRGAAPPRQETRQEARQPEKPRPRAKTNGATAPRQAPRAGYLAVHSVEKS
                     FGTRQVVRGVSIYVRRGEAVGLLGPNGAGKTTVFYMITGLIKADRGAIELDGHDVTSL
                     PMYQRARLGIGYLPQEASIFRGLTVEQNIRAVLEVVEPNRKKREAELNALLDEFNIGR
                     LRKTPSIALSGGERRRVEIARALATRPNYMLLDEPFAGIDPIAVGDIQDLVRHLTNRG
                     IGVLITDHNVRETLGLTDRAYIVYAGEILTEGSPDEIVNNPDVRRLYLGEEFRL"
     gene            complement(2505..3206)
                     /locus_tag="VGA15_16250"
     CDS             complement(2505..3206)
                     /locus_tag="VGA15_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX9589283.1"
                     /translation="MMFSARSTQIRRIAHGAYRVAFAVAIIAAGEACAQSAVQGVPNA
                     MQGFSQNRDQPIQIEAATLEMRDKKKEATFAGNVKVVQGDTTMTSKILVVFYDSTTQQ
                     ATTPAANSKAAAKPAPIQSATPGPGGSSSIKRLEAKGSVVVTQKDQVVTGETAVFDTR
                     ANLITMLGGVVLTQCKNVLRGDRLLVDMTTGVSRVESDNGKVQGLFIQSQGCGPAATG
                     SQAPLPSVGAPNKPK"
     gene            complement(3214..3930)
                     /gene="lptC"
                     /locus_tag="VGA15_16255"
     CDS             complement(3214..3930)
                     /gene="lptC"
                     /locus_tag="VGA15_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX9589284.1"
                     /translation="MEARFAMAARHSRMVRLLRVAVPAAVILAMTAIVFVSVFNPFRM
                     LLPKLPLDMGNLVVSGTKITMESPHLSGYSTDQRPYELWAKTATQDLTDPDHVDLKTL
                     RAKVLMEDQTTVTMDARNGLFDSKQQLLDLHKDIFLQSSSGYEARLTQAFVDIGKGSV
                     TSEEHVDVKLLNGTLTADKLRITGGGEVVRFEGNVVMNLDKLGDEGASGQSANASAAV
                     ADSAPSGKSRSSSGKSANPK"
     gene            complement(4133..4759)
                     /locus_tag="VGA15_16260"
     CDS             complement(4133..4759)
                     /locus_tag="VGA15_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX9589285.1"
                     /translation="MTIRLHRGDLPDLTRYTGSVAIDTETMGLHPHRDRLCVVQMSSG
                     DGSADVVQIPKDHGKDQADAPNLKALLADPKITKIFHFARFDLAALYNALGVMPQPVY
                     CTKIASRLCRTYTDRHGLKDLVREVLNIDLSKQQQSSDWGAQTLSEAQLAYAAADVLH
                     LHGLRERLDTMLAREGRTGLAQACFEFLPTRVKLDLQGWETEDIFAHS"
     gene            5033..5788
                     /locus_tag="VGA15_16265"
     CDS             5033..5788
                     /locus_tag="VGA15_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018448358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9589286.1"
                     /translation="MNTEQQRLPRFAVLIDADNTSPQIAAGLFEEVAKFGEASVRRIY
                     GDFSGSQLKSWADILQKYAIDPYQQFAYTKGKNASDIALVIDAMDLLHSGRFDGFCLV
                     SSDSDFTRLASRLREQGADVYGFGAQKTPESFRQACRRFIYTENLLLAAVPSGASTTS
                     KPNSLQPPSAAIPILEKAISQIESEDGWVMLGPVGQHISNLFSDFDVRSYGHSKLSDL
                     ARKTGAFDVEKIEGGHMRIRAKPANDGAKAKTK"
     gene            complement(5834..6223)
                     /locus_tag="VGA15_16270"
     CDS             complement(5834..6223)
                     /locus_tag="VGA15_16270"
                     /inference="COORDINATES: protein motif:HMM:NF025453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HEX9589287.1"
                     /translation="MKRSLDIAAIVLTSLLLLGFAVLSVRGFIDPQPASARFGIQVSD
                     AAGALFYRVYLSRNLVIAASGAIFLLTRQWTPLAVILTVCATLPLFDMSFLSLSGVTP
                     PPLHPVALALIILSAGLMWRRALPTRA"
     gene            complement(6296..6727)
                     /locus_tag="VGA15_16275"
     CDS             complement(6296..6727)
                     /locus_tag="VGA15_16275"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9589288.1"
                     /translation="MPSLTEEQARAAIAPWYGLFNVASRGDVKTIQEQILTEDYESCA
                     GYLPGECWGRETSIKVVSNFSNTIPDMKFDIKEVLVAGDRVIVRGEVTGTPAGDLFGV
                     PHSGKSFRIMAVDIQTIKDGRIAKTFHMENWLSAIGQLRAK"
     gene            6879..>7220
                     /locus_tag="VGA15_16280"
     CDS             6879..>7220
                     /locus_tag="VGA15_16280"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9589289.1"
                     /translation="MAKATDSKGKTLAAAAKLFRQQGYHGTALQDILAAGGSPRGSLY
                     FHFPKGKEEIGGAALTLAGEALRQGIAKAAEASENAEMFLTRIVRAMASDLEKSDYKE
                     GCPIATTALETA"
BASE COUNT         1406 a         2180 c         2198 g         1436 t
ORIGIN      
        1 cgagacggcg aatttcgccg atcatgtcgg tgatgtcttc atcctcgaca ccgcagaatt
       61 ttcgcaacgc cgcgatatcg cgcttcgcca gaagatccag atgctcgacc agcgcctgca
      121 tcgcagggtc gtaacgattg agctcgcgca gctggatcgc caggcattcg ctcaggcttc
      181 gcgcacagac gccgggcgga tcgaatttct gcagcaccgc gagaaccgct tcgacgtctt
      241 tttgggaggc gccgagccgc tcggcggcct gccccagatc gggcggcacg taacccgcct
      301 cgtcgaccag atcgatcaga tattgcccga tcatgcgctg cgcaggtgcg gcaaaagcca
      361 ccgccagttg ctcggcaaga tgactgccga gcgtaatctc ggcagccaca aaggcttcga
      421 gattgtaact gtcatcgttg gaggcgccgc caccccattc ggtgtaggcg gtcggcgcgg
      481 cgtcctgcgc ggtgcgcgcg gcggcctcgg cgggctcctc ggaaaacacg ttgtcgaggc
      541 cggtatccag ggtctgctcg atctccgcgc ggctgccgag atcgctgctc atccattctt
      601 cctgggccgg ctcgaagcct tcgccggtgg aaccgtgggc catctcgtta cgttcggcgg
      661 cgacgtcgtc gccgtaggcc gaaccggtat cgtcacgatc ggaaaattcg gcccgctccg
      721 gcgtgggttc gcccgcgacc ggtggctcgg tgccatcact ggcgcgttcg agcagcggat
      781 ttcgctccag ttcctcttcg acgaaggccg aaaggtcgag atttgacagt tgaagcagtt
      841 tgatcgcctg catcagctgc ggcgtcatca ccagcgactg ggactgtcgg aactctaatc
      901 tctgtgtcag agccatcgaa gcaagagccg atccaagaaa gttggtccga ttcttgctta
      961 aactagtcca cggccgatgt acacggcttg acgaatggga aaattggact atagcgcgat
     1021 cagaaaaact gtgagcgggt ttgtgcccgg tcgctcaata aaatattaaa cgggcgcatg
     1081 accttagctg taaaccgatc gcttggctct aaaccggtca cagatcggtg ggtcatgcgc
     1141 taaagtcgga actcctcgcc aaggtaaaga cggcgtacat ccggattgtt aacgatctcg
     1201 tctggactgc cttctgtcaa gatctcccca gcatagacga tgtaggcgcg atcggtcagt
     1261 cccagcgttt cccgcacatt gtggtcggta atcagcacgc cgatgccccg attcgtaaga
     1321 tgtcggacaa ggtcctgaat atcgcccacc gcgatcggat cgatcccggc gaacggttcg
     1381 tcgagcagca tgtaattggg gcgggttgca agcgcccgcg cgatctcgac ccgccgccgc
     1441 tcgccgccgg acagtgcaat cgagggtgtt ttgcgcagcc gcccgatgtt gaactcgtcg
     1501 agcagggcgt tgagttcggc ctcgcgcttt ttcctgttgg gctcgacgac ctccagcacc
     1561 gcacggatgt tctgctccac cgtgagaccg cggaagatcg aagcttcctg cggcagatag
     1621 ccgatcccaa gccgcgcgcg ctgatacatc ggcaggctgg taacgtcgtg gccatcgagt
     1681 tcgatcgcgc cgcgatccgc cttgatcaga ccggtgatca tgtaaaaaac cgtggttttc
     1741 cccgcgccgt tcggacccaa caagccaacc gcctcgccgc ggcggacata aatgctgacg
     1801 ccgcgtacga cctggcgggt gccgaaactc ttttccacgc tatgcacagc cagatagccc
     1861 gcccgcggcg cctgccgcgg cgcagtggcg ccattggttt tggcgcgcgg gcgcggtttt
     1921 tccggttgac gcgcttcttg acgcgtttcc tgcctcgggg gagcggcacc acgcgagccg
     1981 tcgcgggcga ggggaggcgc gtcgcgcacc tggttctgct gcagcatctc catcgcgtcc
     2041 tcaagcgagg tgatgtcgcc gcgcgtgcgc gcaaatcccg gcggattgcg tttcgccggg
     2101 cgccgacgga acatgccgag taaatccacc atgcccgctt cgctcagcct ttcacgggat
     2161 ttcgcgaccc gccgcggcga attcgattcg ctcgcgcaac atggatcaat ggatgcccct
     2221 tgcccagcaa aacaccctcg cggtgagggc ggatcgcgtc gttagcgcat gatccgccaa
     2281 agcatgccct cgggcctgac ccgggggtgt cagcggtttg gcgaaaaaga tcatgcgctc
     2341 tttcaatggg ttagagttcg atcggacgtg aaaccagatt ccaccgccgg ctcagctccg
     2401 tcggcgagtt ttttgctgat cgcactctgc gccgttgcgt agataaaggc tggcgccacg
     2461 ggcttcaacc tcgccgccgg cggctagcaa acaagtgatt gatgttattt gggtttattt
     2521 ggggcaccga cggacggcag aggcgcttgc gagccagtcg ccgccggccc acagccttgt
     2581 gactggatga agaggccctg gacctttccg ttgtcggatt cgacccgcga tacgccggtc
     2641 gtcatgtcca ccaaaagacg gtcgccgcgc aacacgttct tgcattgggt cagtacgacg
     2701 ccgccgagca tggtgatcag gtttgccctg gtatcgaaaa cggcggtttc gccggttacg
     2761 acctgatcct tctgggtcac gaccacgctg cctttggcct cgaggcgctt gattgaagaa
     2821 ctgccgccgg gacccggggt cgccgactgg atcggagccg gtttcgcggc ggccttcgaa
     2881 ttggcggccg gcgtggtggc ctgttgtgtg gtcgaatcgt agaacaccac gaggatcttg
     2941 gacgtcatgg tggtgtcacc ctgcaccacc ttcacattgc cggcgaaagt cgcttccttt
     3001 ttcttgtcgc gcatctcgag cgtggcggct tcgatctgga tcggctgatc gcgattctgc
     3061 gaaaatccct gcatggcatt gggcacgccc tgcaccgcgc tctgcgcaca ggcttcgccg
     3121 gcggcgatta tggccactgc gaatgccacc cgataggcgc catgcgcgat ccgccgaatc
     3181 tgggtactgc gcgcagaaaa catcatgatc gaatcatttc gggttggcgg atttgccgga
     3241 gctggatcgc gatttgcctg acggggcaga gtctgccacc gcagcggatg cattcgcaga
     3301 ctggccggac gcgccctcgt cacccagctt gtccaagttc atgacgacgt tgccttcgaa
     3361 tctcaccacc tctccgcccc cggtaatcct gagcttgtcg gcggtcagcg tgccgttcag
     3421 cagtttgacg tcgacatgtt cttccgaggt caccgagccc ttgccgatat cgacaaacgc
     3481 ctgcgtcagg cgcgcttcat agccggatga agattgcaaa aagatatcct tatgcaggtc
     3541 cagcaattgc tgcttgctgt cgaacaggcc gttgcgggcg tccatcgtga cggtagtctg
     3601 atcttccatc agcaccttgg cccgcaaggt tttgagatcg acatggtccg gatcggtcag
     3661 atcctgggtc gcggtcttgg cccacaattc atagggccgc tgatcggtcg agtaaccgga
     3721 cagatgcggc gattccatcg tgatcttggt gccggagacg accaggtttc ccatgtcgag
     3781 cggcagtttg ggcaacagca tccggaacgg attgaaaacc gacacaaaca cgatagccgt
     3841 catcgccagg atcacggcgg ccggaaccgc aacgcgcagc agccgcacca tccggctgtg
     3901 gcgcgcagcc atggcaaagc gcgcctccat tccggtttga taggcaggat tccgaaccga
     3961 attcacggct gctccaaagc gctggcgtcg ctagcgcagg atccgcgctc tcgccattct
     4021 acccggcatc gccggacaat gcagccttag acctcggctg acgagtgtga ccgaaacggg
     4081 gcgtcagcgg gccgccatgg cgcctcaaaa ggtcttggct caagaacctg gctcacgaat
     4141 gcgcgaaaat gtcctcggtc tcccagccct gcagatcgag cttaacccgc gtcggcagga
     4201 actcgaagca ggcttgcgcc agcccggtgc ggccctcgcg cgcgagcatg gtatcgagcc
     4261 gttcgcgcag gccatgcaga tgcagcacgt ccgcggcggc gtaggccagt tgcgcctcgc
     4321 tcagggtttg cgcaccccag tcgctcgatt gctgttgctt cgacagatcg atattgagca
     4381 cctcgcgcac caggtccttg aggccatggc gatcggtgta ggtgcggcac agccgcgagg
     4441 cgatcttggt gcagtagacc ggctgcggca tcacgccgag ggcgttgtag agtgccgcca
     4501 gatcgaaccg cgcgaaatga aaaatcttgg tgattttcgg atcggcgagc aacgccttca
     4561 agttcggcgc gtcggcctga tccttgccgt gatccttggg gatctgaacc acgtcggcgc
     4621 tgccatcgcc gctcgacatc tgcaccacgc aaagccgatc gcgatgcgga tgcagcccca
     4681 tggtctcggt atcgatcgcc accgaaccgg tgtaacgggt gagatcgggc agatcgccgc
     4741 gatgcaggcg gatggtcatg atggtcggga cctcgtcgaa tcggtacgga ctaagctaac
     4801 gtcgaaatcg tcaacaaacg agggtgcacc acatctcgcc tgtttcggat cgatcatttg
     4861 cgattccccg caagaacaac cgtcgttctt acaagctact gacagataac gagttttggt
     4921 ggcgccgacc cgttgggaag gttctgatgt cgtacatttc aacgcatttt tttcggaatc
     4981 agcagaatta cgccaatatg catcggccgc catctggatc gaaggtgcag tgatgaatac
     5041 cgaacagcaa cgcctgcccc gcttcgccgt gctgatcgat gcggacaaca cctcgccgca
     5101 gatcgcagcc ggattgttcg aggaagtggc gaagttcggc gaggccagtg ttcgccgcat
     5161 ctacggggat ttttccggat cgcagctcaa gtcgtgggcg gatatcctgc aaaagtacgc
     5221 catcgatccg taccagcagt tcgcctacac caaaggcaag aacgcgtccg acattgcgct
     5281 ggtcatcgac gcaatggacc tgctgcatag cggccggttc gatggctttt gtctggtgtc
     5341 ttccgacagc gatttcacgc ggctcgcctc acggctgcgc gaacagggcg ctgacgtcta
     5401 cggtttcggt gcgcagaaaa caccggagag ttttcgacag gcatgccgcc ggtttatcta
     5461 cactgagaac ctgttgttgg cagctgtacc gtccggcgcg agtacgacat cgaagccgaa
     5521 ttcgttgcag cctccgagcg ccgcgatacc gattctggaa aaggccattt cacagattga
     5581 gagcgaagat ggttgggtga tgctcgggcc ggtcggtcag cacatctcaa acctgttctc
     5641 ggatttcgac gtgcgctctt acggccatag caagctcagc gatctcgcgc ggaaaacagg
     5701 cgccttcgat gtcgaaaaga tcgaaggtgg acatatgcgc atccgggcca agcctgccaa
     5761 cgacggcgcg aaagcaaaga cgaagtgaca tttcgttgcg cccgaactcg gttcggactg
     5821 agattcaggc ggatcatgcg cgagttggga gcgcccgccg ccacatcagc cctgctgaca
     5881 aaatgataag cgccagcgcc acggggtgaa ggggcggcgg cgtcacgccg ctgagcgaca
     5941 ggaaggacat gtcgaacagc ggcagggtcg cgcagaccgt cagaataacg gccagcggcg
     6001 tccattgccg cgtgagcagg aaaatcgcgc cggaagcggc gatgacgaga ttgcgggaca
     6061 gatacacgcg ataaaacagg gcgccggcgg catcggacac ctgtatgcca aaccgggccg
     6121 aggccggttg cggatcgatg aacccgcgca cggacagcac cgcgaaccca agcaacaata
     6181 gcgaggtcaa aacgattgcc gcaatatcga gcgatctttt catgttcgtt cctccgtgag
     6241 gtgagcgcgg gaccgcctgc cagcggcttt catgaggccc ttgacggggc cgtgtttatt
     6301 tggcgcggag ctgtccgatg gcgctgagcc agttttccat gtggaaggtt ttggcgattc
     6361 tgccgtcctt gatggtttga atatcgaccg ccatgatccg aaaactcttg ccggagtgcg
     6421 gcacgccgaa caggtcgccg gcaggcgtac cggtgacctc gccacggacg atgacccgat
     6481 cgcccgcgac cagcacttcc ttgatgtcga atttcatgtc cggaatggtg ttcgagaagt
     6541 tcgagaccac cttgatcgac gtctcgcggc cccagcattc ccccggcaga tagccggcgc
     6601 aggactcgta gtcctccgtc aggatttgct cctggatggt tttgacgtcg ccccggctcg
     6661 cgacattgaa caggccgtac cagggcgcga tggcggcgcg ggcttgttct tcggtgaggc
     6721 tgggcatggc ttttttcctg aaggttgatg cacgtttggg acgaccgcgc gcgatccggg
     6781 gccgggcggg gaccacaatg cgattaaacc catctatgta ggtcactcta tatactatga
     6841 aggtcgacat actgatttgc aactgatatt ttcgaaatat ggcaaaagct actgattcca
     6901 aagggaaaac actggccgcc gcggcaaagc tgtttcgcca gcagggctac catggcacgg
     6961 ccctgcagga cattctggcc gccggcggct caccgcgcgg ctcgctctat ttccattttc
     7021 ccaaaggcaa ggaggaaatt ggcggcgccg cgctgacact tgctggcgaa gcgctgcgcc
     7081 agggcatcgc gaaagccgcc gaggcgtccg agaacgccga aatgttcctc acccgcatcg
     7141 tgcgcgccat ggcctctgat ctggaaaagt ccgattacaa ggaggggtgc ccgatcgcga
     7201 ccacggcact tgaaaccgcg
//
LOCUS       DASWGM010000571         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1856288,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000571 DASWGM010000000
VERSION     DASWGM010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1856288"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VGA15_16285"
     CDS             <1..618
                     /locus_tag="VGA15_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX9589290.1"
                     /translation="KHVAPFGGREARLGTNPISIAVPSNLEAPFYLDMATSAVAAGKI
                     QLAAARGEEIPQGWIVDSDGRQTTDPRQFRKGGALLPLGGTEGYKGSGLAAMVEVLCG
                     LLTGLGFGVEPTGRHNDGCFMAVFNVAAFRPLKDFKQEVAEFARYLKATPPSEGSPGV
                     FYPGEVEYIREQQRRVSGIDVEGATWEKLRSLAGEYQLAAELDLA"
     gene            651..2018
                     /locus_tag="VGA15_16290"
     CDS             651..2018
                     /locus_tag="VGA15_16290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9589291.1"
                     /translation="MTRQMAMVGFLQAQNCTNLPSSWRHPESRDDSMSADYYQEIARI
                     LESGKFHMAFFDDRLAMPDRYGNDHRHTVEYGIRCVKMDPIVVLTTMGMVTEKLGLAS
                     TCSTTYYEPFDVARRFATLDLMTGGRAAWNVVTSVNDGEAHNMGKDAHLDHDFRYDRA
                     DEFMEVVLGHWDSWEDGSLLIDKKSGRFADPDKVKRLDHNGQFFKSRGPFTVPRSQQG
                     HPVIIQAGASGRGQRFAGRWGEVIFTAARNLASARQGYDAIRNEAAKAGRDPDQMFLC
                     NLVTPVSGATRSEAEDKMALIETLPLEIDALSLLSEGLNYDFASKHIDEPLTTEELTG
                     MQGMIGIRDGVLRTSGKTNPSTRDFITFSGRGQTQDAIVGGPKEIADRLEEMFLGRGC
                     DGFVIAATYVPGSYADFVRHIVPELQRRGLFHKDYAGKTLRENLGLPRPAAGAWKSQP
                     RVAAE"
BASE COUNT          417 a          641 c          671 g          358 t
ORIGIN      
        1 aagcacgtcg cccccttcgg cggccgcgag gcgcggctcg gcaccaatcc gatttcgatc
       61 gcggtgccgt ccaatctcga ggcgccgttc tatctggaca tggcgacatc ggcggtcgcg
      121 gccggaaaga tccagctcgc ggccgcccgc ggcgaggaaa tcccgcaggg gtggatcgtc
      181 gacagcgacg gacggcaaac caccgacccc agacaattcc gcaaaggcgg cgcgctgctg
      241 ccgctcggcg gcaccgaagg ctacaagggc tcaggcttgg ccgcgatggt cgaagtgctg
      301 tgcgggctgc tcaccggcct gggttttggg gtcgaaccca ccggccgcca caacgacgga
      361 tgtttcatgg cggtgttcaa tgtcgccgcg ttccgacccc tgaaggattt caaacaggaa
      421 gtcgccgagt tcgcgcggta cctgaaggcg acgccgccgt cggaaggctc ccccggcgtg
      481 ttctatccgg gcgaggtcga atatatccgc gagcagcagc gccgggtttc gggcatcgac
      541 gtcgaaggcg caacctggga aaagctgcga tcgctggccg gcgaatacca actggccgcc
      601 gagcttgatc tggcgtgaca tctcagtcgg ccgacactca ggagagcagc atgacgcggc
      661 aaatggcgat ggtcggattc ctgcaggcac agaactgcac caacctgccg agttcgtggc
      721 gccatccgga atcgcgcgac gattcgatgt cggcggatta ctatcaggag atagcccgaa
      781 ttctggaatc cggcaaattc cacatggcat tcttcgacga tcgcctggcc atgccggatc
      841 gctacggcaa cgatcacagg cataccgtcg aatacggcat ccgctgcgtg aagatggatc
      901 cgatcgtggt gctgaccacg atgggaatgg tgaccgagaa actcgggctc gcttcgacct
      961 gctcgaccac ctattacgaa ccgttcgacg tcgcccgccg cttcgccacc ctcgatttga
     1021 tgaccggcgg tcgcgccgcc tggaacgtgg tgacttccgt caacgacgga gaagcgcaca
     1081 atatgggcaa ggacgcgcat ctcgaccatg atttccgtta cgaccgcgcc gacgaattca
     1141 tggaagtggt gctcggccat tgggattcct gggaggacgg ttcgctcttg atcgacaaga
     1201 agtcaggccg gttcgccgat cccgacaagg tcaagcgcct cgatcacaac gggcagttct
     1261 tcaagtcgcg cggaccgttc acggtgccgc gctcgcaaca ggggcatccc gtcattattc
     1321 aggccggcgc cagcggccgc ggccaacgct ttgcggggcg atggggtgag gtgatattca
     1381 cggcggcgcg caatctggcc agcgccaggc aaggctacga cgccatccgg aacgaggccg
     1441 ccaaggcagg ccgcgatccc gaccagatgt ttctctgcaa tctggtgacg ccggtgtccg
     1501 gtgcgaccag gtccgaggcc gaagacaaga tggcgctgat cgaaacgctg ccgctggaaa
     1561 tcgacgcgct gtcgctgctg tcggagggac tgaactacga ctttgcctca aagcacatcg
     1621 acgaaccgct gacgacggaa gagctgacgg gaatgcaggg aatgatcggc atccgcgacg
     1681 gcgtgctgcg gaccagcggc aagaccaatc ccagcacgcg cgatttcatc accttctcgg
     1741 gtcgcggcca gacccaggac gccatcgtcg gcggcccgaa ggagattgcc gaccggctgg
     1801 aagaaatgtt tttgggtcgc ggctgcgacg gttttgtcat tgcggcgact tacgtgcccg
     1861 gctcgtacgc ggatttcgtt cgccacatcg tcccggaatt gcaacgccgc gggttgtttc
     1921 acaaagacta cgccggcaag accttgcgcg aaaatctcgg cctgccgcgt ccggctgccg
     1981 gtgcatggaa aagtcagccg cgcgttgcgg ccgaataaca aaaaagggag taggcatgcg
     2041 ttggctcaaa tttacggcgg gcggacaaac ctcgtggggg atcgtcg
//
LOCUS       DASWGM010000572         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1287418,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000572 DASWGM010000000
VERSION     DASWGM010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287418"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VGA15_16295"
     CDS             <1..384
                     /locus_tag="VGA15_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9589292.1"
                     /translation="FAKVERLAVRHQFRRSRVSFKLVQASFDYIKRKGFKKIYGHAQD
                     RLVNFWAHFGGKPLGHNRKITFSDFSYTEMVLEIEPGPDAITLDSDPYVIIRPEGDWD
                     RPGVLDISSARPVTSPLRAAALAET"
     gene            399..962
                     /locus_tag="VGA15_16300"
     CDS             399..962
                     /locus_tag="VGA15_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX9589293.1"
                     /translation="MMVSVHDDPPILICADLQIEYLTEGRRHVIADADAITSRCLELL
                     TLWRNNLWPVMHLKRIAQAAWFNPASNLTDWIAELKPRPGELAFEHPLPSAYSSTRFA
                     EYMSNMKNIRCVLSGFSLDETILSTVVDGFHRGHRYQVVSDAVACRRPGIDDVASYKM
                     AVVRVIGNFAGIQGSSDVVGASGKIAV"
     gene            complement(1184..2332)
                     /gene="rnd"
                     /locus_tag="VGA15_16305"
     CDS             complement(1184..2332)
                     /gene="rnd"
                     /locus_tag="VGA15_16305"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX9589294.1"
                     /translation="MELITTTSELATVCTRLGKHPVITVDTEFLRETTYYPLLCVVQM
                     ASAEEAVVIDTLASGIDLKPFFELMADEKVLKVFHAARQDIEIVWHQAGIVPHPIFDT
                     QVAAMVLGYGDSIAYDQLVERIAGHRPDKTHRFTDWSRRPLTEEQVHYAVSDVTHLRD
                     VFTALDADLKKRGRSDWVSEEMEVLTSPKTYDFHPERAWERLKTRVRKPKELAVLMEV
                     AAWREQEAQSRDVPRSRVLKDDAVGDIAVHAPTTLERLANLRSLPKGFDRSKWGADIV
                     AAVQRGLARDPATLPKLEKPRGNSNGAAIVELLKVLLRMTSERHAVASKVIATVDDLE
                     QIAADDNADVGALHGWRRELFGNAALSLKQGRLALAIEKGRVVRVDRN"
BASE COUNT          519 a          831 c          838 g          529 t
ORIGIN      
        1 ttcgccaagg tcgaacgtct cgcggtccgc catcagttcc ggcggtcgcg cgtatcgttc
       61 aagttggtgc aggccagctt cgactacatc aagcgcaagg gcttcaagaa gatctacggc
      121 cacgcgcaag accggctggt gaacttctgg gcgcatttcg gcggcaagcc tctcggccac
      181 aaccgcaaga tcaccttttc cgatttctcc tacaccgaga tggttcttga aatcgagccg
      241 ggccccgacg ccattaccct cgatagcgat ccttatgtga tcatcaggcc ggagggcgat
      301 tgggacaggc ccggcgttct cgacatttcg tcggcgcgcc cggtgacatc tccgctgcgc
      361 gcggcagcat tggccgaaac atgatctcga atcgaaagat gatggtttcc gtgcatgacg
      421 atccgccgat cctgatctgt gccgatcttc aaattgaata tctcaccgaa ggccgccgcc
      481 acgtcatcgc ggacgccgac gcgatcacgt cgcgttgcct cgaactgctg acgctctggc
      541 gcaacaacct ctggccggtc atgcatctga aacggatcgc gcaggccgca tggttcaatc
      601 cggcctcgaa cctgacggac tggatcgcgg aactgaaacc gcggccgggg gagctcgcct
      661 tcgaacaccc gctgccgtcg gcatatagtt cgacgcggtt cgcggaatac atgtcgaaca
      721 tgaaaaatat tcgctgcgtg ctgtcgggat tttccctcga tgaaacgatt ctgtcgaccg
      781 tcgtcgacgg gtttcaccgc ggccaccgct atcaggtcgt cagcgacgcc gtggcttgcc
      841 ggcgcccggg tatcgacgat gtggcgtcgt acaaaatggc ggtcgtcagg gttattggca
      901 actttgccgg gatccaggga agctccgacg tggtcggagc aagcggtaag attgcggtat
      961 gatcaggctc gaatccatcg ccttgaggca tccgttacct tgcgagccgt tcggcgaatt
     1021 cgatgatggt gcgccgagcg gcgggaccgg cgcaaggtgc tgggaagcgc tggagcgatg
     1081 atgccgcgcg gtagcgcgtc cgcgcgccga tccaaaacaa ctcctttgat cggggccgat
     1141 ccgggcagcc aatcagctcg agcgtgcggg cagcgcgaga acgtcagttc cgatcgaccc
     1201 tgacaacccg gcccttttcg atcgcgagcg ccagtcgtcc ctgtttcagc gacagggccg
     1261 cgttgccaaa cagctcgcgg cgccagccgt gcagcgcgcc gacgtcggcg ttgtcgtcag
     1321 ccgcgatctg ttccagatcg tcgacggttg cgatcacctt gctggcgacg gcgtggcgct
     1381 ccgacgtcat ccgcagcaga actttaagca gctcgacgat cgccgcccca tttgaattgc
     1441 cgcgcggctt ttcgagtttg ggcagtgttg ccggatcgcg tgccaggccg cgctgcacgg
     1501 cggccacgat gtcggcaccc catttcgagc ggtcgaaacc ctttggcagc gaacgcagat
     1561 ttgccaaccg ctcgagagtg gtcggcgcat ggacggcgat atcgccgacc gcatcgtcct
     1621 tgagaacccg gctgcgcgga acgtcgcggc tctgcgcctc ctgttcgcgc caggcagcta
     1681 cctccatcag caccgcaagc tccttcggct tgcgtacccg cgtcttcagc cgctcccagg
     1741 cgcgttcggg gtgaaagtcg taggtctttg gcgaggtcag aacctccatt tcctcgctca
     1801 cccaatcgct gcggccgcgc ttcttgagat cggcgtcgag agcggtgaac acgtcgcgca
     1861 gatgggtgac gtcggagacg gcgtagtgga cctgctcttc ggtcaggggg cggcgcgacc
     1921 agtcggtgaa gcggtgggtc ttgtcgggcc ggtggccggc gatgcgctcg accaactggt
     1981 cgtaggcaat ggaatcgcca tagccgagca ccatggccgc gacctgggtg tcgaagatcg
     2041 gatgcggaac gatgccggcc tggtgccaga cgatttcgat atcctgacgg gcggcgtgaa
     2101 aaaccttcag caccttctcg tcggccatca actcgaagaa cggcttgagg tcgatgcctg
     2161 aagccagcgt atcgatcacg accgcttcct ccgcgctcgc catttgcacg acgcagagca
     2221 gcgggtaata ggtcgtctcg cgcagaaatt cggtatcgac cgtgatgacc gggtgtttcc
     2281 cgaggcgggt gcaaacagtg gcaagttccg aagtggtggt gataagttcc atgaacagtc
     2341 cataacgctt cttaagaggg cgttctgccg aaagcgctga tatgtcaagg gtcttgcgac
     2401 gaattcaagc cttgcatttc cgcgctgcca cgacgtatcg gcgagactca agctaccggg
     2461 gaccgtagct gacggcggcc cggatccggc caaaccatgc gtttggaatg gggatcagtc
     2521 aggtgtcgct gatgtgccgc gcaggacgtc gtcccgacgg aatagccagc acgacgacgc
     2581 caagcgccag catcgccaat cccatgaatt cgaatatcaa tgcgtcaaac acaacaattc
     2641 ccccgccaaa ccgatagact tgtcgggaag ggattgaacg tttcttaatt cagcgagcag
     2701 ggcacgcggc gctccgt
//
LOCUS       DASWGM010000573         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_322718,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000573 DASWGM010000000
VERSION     DASWGM010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322718"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1326)
                     /locus_tag="VGA15_16310"
     CDS             complement(112..1326)
                     /locus_tag="VGA15_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX9589295.1"
                     /translation="MIDEIAVRTRYEALLLCLDERGRRLFAAVEARTAGYGGIAAVAR
                     ATGIAPSTIGRGLKDLAASGFPEGSVRRAGSGRQPLTTADPTLLEDLRRLVEPATMGD
                     PTRPLLWVSKSHAKLAAALRTMGHQIATSSMPKLLGELKYRRQVNRKTKEGSHNPDRN
                     GQFEHINKQAMALQAAHQPVISVDTKKKELIGDYKNPGSDYRPQGCPDAVNMHDFADK
                     ELGKVVPYGVYDIAADAGCVSVGIDHDTAEFAVNAIQRWYDTMGRERYPKADRLMITA
                     DGGGSNGTRVRLWKIKLQEFADATGLTLSVCHYPPGTSKWNKIEHRLFCHISQNWRGK
                     PLTSRLTVVELIAATTTKTGLQVRCELDTNVYAKGIKVTDAEMAALNITGNVFHPEWN
                     YTISPRSPKPPT"
BASE COUNT          264 a          488 c          471 g          339 t
ORIGIN      
        1 ctgtcaaagt acgatggcaa aacgcagtgg tatgggccta gtgttgtgac tctgacggat
       61 cgttctgtca ttccgggacg cgagctaagg cgacgcccaa caattattgc gtcaagttgg
      121 tggtttcggc gatctgggtg agatggtgta gttccactcg gggtggaaga cgttacccgt
      181 gatgttgaga gcggccattt ctgcgtcagt caccttgatg cccttggcat agacattggt
      241 gtcgagttcg catcggacct gcaagccggt tttcgtggtg gttgcggcga tcagctcgac
      301 cacggtcaaa cggctggtca gtggtttgcc gcgccaattc tggctgatgt gacagaacag
      361 gcggtgctcg atcttgttcc atttcgacgt gcctggggga tagtggcaca ccgagagggt
      421 caatccggtc gcgtcggcaa attcctgcag cttgatcttc cacagccgga cgcgggtccc
      481 attggagccg ccgccatcgg cggtgatcat caatctgtcg gccttggggt agcgctcgcg
      541 gcccatcgtg tcgtaccagc gctggatggc gttgaccgcg aattcggcgg tgtcgtggtc
      601 gatgccgacg ctcacgcagc cagcatcggc agcgatgtcg tagactccgt agggcacgac
      661 cttgccaagc tctttgtcgg cgaagtcgtg catgttgacc gcgtccggac aaccttgcgg
      721 ccggtaatcg ctgcccgggt tcttgtaatc tccgatcagt tccttcttct tggtatccac
      781 ggagatcacc ggctgatggg ccgcttgcaa ggccatcgcc tgcttgttga tgtgctcgaa
      841 ctggccgtta cggtcgggat tatggcttcc ttccttggtc tttcggttga cctgccggcg
      901 gtatttcagc tcacccagca gcttgggcat gctgctcgtg gcgatctgat gccccatcgt
      961 gcgcagtgcc gcagcaagct tggcgtggct cttcgacacc cacagcaacg gccgcgtcgg
     1021 atcacccatc gtcgccggct cgaccagtcg acgaaggtcc tccagcagcg tcgggtccgc
     1081 cgtcgtcagg ggctgccgcc cgctgcccgc acgacgaacc gatccctcgg gaaatccaga
     1141 cgcagccaga tctttcaacc cgcggccgat ggtactcggc gcaatccccg tcgcccgcgc
     1201 caccgcggca atgccgccgt aacccgccgt ccgagcctcc accgccgcaa acaagcgccg
     1261 tcctcgctca tccaggcata gaagcagggc ctcgtagcgt gtgcgaaccg caatctcgtc
     1321 gatcatccag cgacgataca cagcacacgc acgtccggga atcccctgcc cgtagcctat
     1381 ccctcgccac ggcaagccga ttcacttatt cttcggcgtc gcctaagtat ttggttggtc
     1441 gaaggtctga ccccgcacgg cgaagcgctg ccatgagctt ttgcggggcg ccgtcaggat
     1501 cggccaatgg ccgatcctga cggcgttcgc cgggactagt gctccgattc cgaagttcgc
     1561 at
//
LOCUS       DASWGM010000574         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_70219,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000574 DASWGM010000000
VERSION     DASWGM010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70219"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VGA15_16315"
     CDS             <1..263
                     /locus_tag="VGA15_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX9589296.1"
                     /translation="VVVKRIDAEQKTKGGIIIPDSAKEKPSQGEITAIGPGGRDESGK
                     LILIDLKVGDRVLFGKWSGTEIKLDGEELLIMKESDIMGVLA"
     gene            424..>1793
                     /gene="groL"
                     /locus_tag="VGA15_16320"
     CDS             424..>1793
                     /gene="groL"
                     /locus_tag="VGA15_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475125.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9589297.1"
                     /translation="MAAKDVKFSGDARERMLRGVDILANAVKVTLGPKGRNVVIEKSF
                     GAPRITKDGVTVAKEIELEDKFENMGAQMLREVASKTNDTAGDGTTTATVLAQAIVRE
                     GAKSVAAGMNPMDLRRGIEIAVAAVVKDLEKRAKPVASSSEIAQVGTISSNGDTAIGK
                     MIAQAMQKVGNEGVITVEENKSLETEVDIVEGMKFDRGYLSPYFITNAEKMTAELEDA
                     YVLLHEKKLSGLQAMLPVLEAVVQSGKPLLIVAEDVEGEALATLVVNRLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGQLISDELGMKLESVTINMLGRARKVVIDKENTTIVS
                     GAGKKKDIEARVGQIKAQIEETTSDYDREKLQERLAKLAGGVAIIRVGGATEIEVKEK
                     KDRVEDALNATRAAVQEGIVPGGGVALLRAKKAVGRIHNDNSDVQAGINIVLKALEAP
                     VRQIAE"
BASE COUNT          401 a          539 c          584 g          269 t
ORIGIN      
        1 gtgtcgtggt caaacgtatc gacgccgaac aaaagaccaa gggcggcatc atcattccgg
       61 atagcgccaa ggagaagccc tcgcagggcg agatcacggc gatcggtccg ggcggccgag
      121 acgaatccgg caagctgatc ctgatcgacc tcaaggtcgg cgaccgtgtg ctgttcggca
      181 agtggtcggg caccgagatc aagctcgacg gtgaggaact tctgatcatg aaggaatccg
      241 acatcatggg cgtgctggcc tgagataaac caaaagcccc gcggcgctcc ctgtcgtcat
      301 gcccggacct gatccgggca tccatcattc ttcgaaaacg cgatggattg ccgggtcaag
      361 cccggcaatg acggtcaaga aacgcggcgg gcgaatgtat ccaatcactt aggagtcgaa
      421 acgatggctg ccaaggacgt taaattttcc ggagacgcgc gtgaacgcat gctgcgcggc
      481 gtcgatattc tcgccaatgc ggtgaaggtg acgctgggtc cgaagggtcg caatgtcgtc
      541 atcgaaaaga gctttggcgc gccgcgcatt accaaggacg gcgtcaccgt cgccaaggaa
      601 atcgagctgg aggacaagtt cgagaatatg ggcgcgcaga tgctgcgcga agtcgcctcc
      661 aagaccaacg acaccgccgg cgacggcacc accaccgcca ccgtgttggc gcaggcgatc
      721 gtgcgggaag gcgccaagtc ggtcgccgcc ggcatgaacc cgatggacct gaggcgcggc
      781 atcgaaatcg cggtggcggc cgtcgtcaag gatctcgaga agcgcgccaa gccggtcgct
      841 tcatcatccg aaatcgccca ggtcggcacc atctcgtcca atggcgacac tgcgatcggc
      901 aagatgatcg cgcaggcgat gcagaaggtc ggcaatgagg gcgtcatcac cgtcgaggag
      961 aacaagtcgc tcgaaaccga agtcgacatt gtcgagggta tgaagttcga ccgtggttac
     1021 ctgtcgccct acttcatcac caacgccgag aagatgacgg cggagcttga agacgcctac
     1081 gtgctgctgc acgagaagaa gctgtccggc ctgcaggcca tgctgccggt cctggaagcg
     1141 gtggttcagt ccggcaagcc gctcttgatc gtcgccgagg acgtcgaggg cgaggcgctg
     1201 gccactttgg tcgtcaatcg cctgcgcggc ggcctgaagg tcgccgccgt caaggcgccc
     1261 ggcttcggcg accgccgcaa ggcgatgctg gaagacatcg cgatcctgac cggcggtcag
     1321 ctgatctcgg acgaactcgg catgaagctc gaaagcgtga ccatcaacat gctcggccgc
     1381 gcccgcaagg tcgtgatcga caaggaaaac accaccatcg tcagcggcgc cggcaagaag
     1441 aaggatatcg aagcccgggt cggccagatc aaggcgcaga tcgaggagac cacctcggac
     1501 tacgaccgtg agaagctgca ggagcgcctg gccaagctcg ccggcggcgt cgcgatcatt
     1561 cgcgtcggcg gcgcgaccga gatcgaggtg aaagagaaaa aggaccgcgt cgaggacgcc
     1621 ctcaacgcca cccgcgccgc cgtgcaggaa ggcatcgtgc cgggcggcgg cgtcgcgctg
     1681 ctgcgcgcca aaaaggccgt cggccgtatc cacaacgaca attccgacgt ccaggccggt
     1741 atcaatatcg tgctgaaagc gctggaagcg ccggtccgcc agatcgccga gaa
//
LOCUS       DASWGM010000575         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2563082,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000575 DASWGM010000000
VERSION     DASWGM010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2563082"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VGA15_16325"
     CDS             complement(<1..466)
                     /locus_tag="VGA15_16325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9589298.1"
                     /translation="MAEVTLPRPLSETDDRGRFDCGRESLNHWFLRHAWANHAAGISR
                     TNVICDAATAEIIGYVTLSAAQVERAFLPKSQQRNKPDPVPATLLGQLAIHKDHQGKG
                     HARSLLLFALRAALRASREIGSFGVITHPLDDSVRAFYGRWGFQDLPFDPHRA"
     gene            complement(454..717)
                     /locus_tag="VGA15_16330"
     CDS             complement(454..717)
                     /locus_tag="VGA15_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HEX9589299.1"
                     /translation="MTTLVLSVRVNEEERALLEAASALARTSLSDFIRRKALDAAEAD
                     ILERRIVTIPAKDWEGFEAWANRPPEEIAGLKDLARKTPTWQK"
     gene            1103..1369
                     /locus_tag="VGA15_16335"
     CDS             1103..1369
                     /locus_tag="VGA15_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX9589300.1"
                     /translation="MDFEWNGAKELANRKKHGVDFRTAAKVFLDPYVIEFDDLDAAGE
                     LRFNAIGLVDGRMLFVTYTMRDDVVRIISARGAEPHEKRKYHEI"
     gene            1341..1697
                     /locus_tag="VGA15_16340"
     CDS             1341..1697
                     /locus_tag="VGA15_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9589301.1"
                     /translation="MKKGSITRFKLNPKKLPKTDWRAFDAMSDEERHRAAVSDPDAPP
                     ATEAQLARARRVPTVRALRKRLNLTQEEFAARFHLPLGTVRDWEQGAHRPDKAAQVLL
                     TVIARDPDAVARALES"
     gene            complement(1843..2798)
                     /locus_tag="VGA15_16345"
                     /pseudo
     CDS             complement(1843..2798)
                     /locus_tag="VGA15_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          565 a          908 c          953 g          558 t
ORIGIN      
        1 ttgcgcggtg agggtcgaag ggcagatcct ggaaccccca tcggccatag aacgcgcgca
       61 cagaatcgtc gagcgggtgt gtgataacgc cgaagctgcc gatctctcgg gaggctctga
      121 gcgccgcgcg aagcgcgaac agaaggagcg aacgggcgtg gccctttcct tgatgatcct
      181 tgtggatcgc aagctggccg agcagtgtcg ccggcactgg gtcgggcttg ttgcgctgct
      241 gtgacttggg caagaatgcc cgctcgacct gcgcggcgct caaggttacg taaccgatga
      301 tctcggccgt ggcggcgtcg cagatgacat tggtgcgcga gatgccggcg gcgtggttgg
      361 cccaagcgtg acgcaggaac caatggttca gcgactcgcg gccgcaatca aatcggccgc
      421 gatcgtctgt ttcagagagc ggacgcggta gcgttacttc tgccacgtgg gcgtcttgcg
      481 cgcgagatcc ttcagcccgg caatctcctc cggcggacga ttcgcccaag cctcgaaccc
      541 ttcccagtcc ttggccggga tggtgacgat ccgccgctca aggatgtcgg cctcggccgc
      601 gtcgagcgct ttgcgccgga taaaatcgct gaggctggtg cgcgccagcg cggacgcggc
      661 ctcgagcagg gcgcgctctt cctcgttgac gcgtacgctc agcaccaaag tggtcatggc
      721 acgtgctcct gtggactccg cgagtgtatg acaatagcat acatcggata ccgccagcaa
      781 tcccggcgat ggtgtttcgg atgtgccggg aggcggagat agcgaaatcc cgtaccgccg
      841 cgagtcccac gcggcgccgc gacaagcggt tcacgaggcg ttatcgggac aaagcagcga
      901 acgattgcgc tgcaacggga cgtcgcggga cgcgacatcg tgctctggcg tgcagtggcg
      961 cgccattcta cgccagagcg cgatatcacg gcctatggcc gccctcccaa cgcaattgtt
     1021 ctccgctaag ccacccacgg cacggtcatg agggccttgc acaaagtact gattatcagt
     1081 acattcgagg tcggggctcg caatggactt cgaatggaac ggcgccaagg agctggccaa
     1141 ccggaagaag cacggtgtcg actttcgaac ggccgcgaaa gtgttcttgg acccgtacgt
     1201 gatcgagttc gatgacctcg acgccgctgg cgagttacgc ttcaacgcta tcggcttggt
     1261 cgacggccga atgctcttcg tcacctatac gatgagggac gacgtcgttc gcatcatatc
     1321 tgcaagagga gcggagccgc atgaaaaaag gaagtatcac gagatttaag ctcaatccga
     1381 agaagctgcc aaagacagat tggcgtgcct tcgacgccat gagcgacgag gaacgtcatc
     1441 gagccgctgt ttcggacccg gatgctccgc cagcgaccga ggcccagctc gcgcgtgctc
     1501 gccgtgtgcc gaccgtgcgg gcgttgcgca agaggctgaa tttgacgcag gaagagtttg
     1561 cggcgcggtt ccacctcccg ctcgggactg tgcgagattg ggagcaaggc gcacatcgtc
     1621 ccgacaaggc cgcgcaagta ttgctgacgg tcatcgcaag agacccggac gcagtggcac
     1681 gcgcgctcga aagctagccg ttcgatgttg tagcggcttc gaccgccatg gcgtaacgca
     1741 gcgtgctccg ggatagctct gcgtgctcta tggtagcgcc acggcgaaac aatagtgccc
     1801 atcttcatcg gccgtggcgc ccctcctccg atccgcaatc tctcaaaacc gatgcgtctc
     1861 ggattgagca gccgtgttgc gcgcgatctc gtcgatcatg gcgtcgatct cggagatcac
     1921 gaactcgggc gcggcgttct gcaccatgtg gccgacattg ggcaatacga tcagtttcgc
     1981 attcggcacc gcggccgcga acggtcggga atggatgttg gtcgacacgg tcttgtcgac
     2041 gtcgcccgcg atcaccaccg tcggcgccgt gatgttggcg taacgtggcg cctgctctgc
     2101 caccgccgcc ttcagcgtca ccaggtcctg ggcattggcg aggaattcgc gcggacggtg
     2161 tccttgatga agttttccgg cattatctgc ggcaaaaata cgtcgcgcgc gccggaccca
     2221 gcccaaaaaa ggccgagcgg gagcgtgatc gtataggcca gcagcggacc gatcacggga
     2281 gtggtgattg ccttgttgta ccatccgacg ccgccgggcc acggataggc caccggcgcc
     2341 agcatcacca gccctgcgac gcgctgcggg taatcgagcg cgatgcgcgc cccgagcgca
     2401 ccgctccagg aatggaccac caggatcgcc cggctgacgc ccagtctttc cagcgcttca
     2461 tcgatcattc tgccctggat atcgggcgtc gaatcgtcgc gccgcgcgcg cgtgctccag
     2521 ccgtgcccgg gacgatcgat caggatcacc cgccggtttt cggcaagcct gtcgccgagc
     2581 ggttgccgca tcacttgcag attggaactg gcgccatgga tcaaaacgat cgggggaccg
     2641 gcggcgtcgc gcggcccgat atcgacgacg ttgaggcttg cgccggcgac ctcgatggcc
     2701 ttgccctgcg cgggataagt gcgctgcagg gcaagcacac cgatctgcgt ggtcagtgcc
     2761 aggatcgcca gcgccgccac cacaacgact gatatcattt ggaatgtccg gatgagtttt
     2821 ggcaccaatg agttacggtt tcgaaaggtg atggtttcgc cgctcgtgga atcagactag
     2881 agcgggatga cttttcttcg aatcgtcaac ccgctctatc tttttgtttg agcatgatct
     2941 ccgcgcaaac gcttcgcgtt tgtcgcgagg gaaaaccggt accc
//
LOCUS       DASWGM010000576         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1732953,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000576 DASWGM010000000
VERSION     DASWGM010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732953"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGA15_16350"
     CDS             complement(<1..351)
                     /locus_tag="VGA15_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9589302.1"
                     /translation="MAGLYFEQFSVGQTFVHEIRRTVTDMDNILFSALTYNPAAVHID
                     HEYAKSTEFGKPLMNSIFTLGLVIGLSVQDTTLGTTVGNLGMEDTKFPRPVFAGDTLR
                     AETKIVAVRESKSRP"
     gene            complement(595..1464)
                     /locus_tag="VGA15_16355"
     CDS             complement(595..1464)
                     /locus_tag="VGA15_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589303.1"
                     /translation="MPYQSVFRPNLFKGQTIIVTGGGSGIGRCTAHELVALGANVAIL
                     GRTAEKLAEVKAEIEEDGGQILTQVCDIRDEAMVIAAIDAVLGAYGRIDGLVNNAGGQ
                     YRAPMKTISTKGFEAVVRSNLTGGFIFMREVYNRWMEANGGAIVNIIADIWHGWPEFA
                     HSGAARGGMLTLTETAACEWSASGVRVNAVAPGGIVSSGFDTYTPEMQKKLHDFTAGV
                     PLQRFGTEAEISAAVTFLLSPAAAYVTGSCIRVDGGTPNARTTWKLEAHNRSAPFEGF
                     HRSKLPEALKKAP"
     gene            1466..>2322
                     /locus_tag="VGA15_16360"
     CDS             1466..>2322
                     /locus_tag="VGA15_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9589304.1"
                     /translation="MFSELIVSAAGGKAAKTSTLYYDMVYSLAQESICESASGPAAGI
                     WNNNATVWLSTHHRDFLLAGRRMVPLGTHDMDKFTTITVEQRDAIEILSLNRPDALNA
                     VTPAMADELIDYFSGLHNRLGTRIVIFRGNGRAFCAGAELGSDAFSPPGPGRPQRQLD
                     MQQRYSRIIRLMRSCPQPIVALVQGAACGAGFSLLLASDVRFATRAARMNAAYIRVGV
                     GGCDMGSGYLLPRLIGLSVASELLLTGRFLEADRAKAVGLVSDVVDDDKLLETGLAFA
                     AEMARASPMG"
BASE COUNT          426 a          722 c          689 g          485 t
ORIGIN      
        1 tgggcgagac ttgctttcgc gcaccgccac aatcttcgtt tccgcccgca gcgtatcgcc
       61 ggcaaagacc gggcgcggaa atttggtgtc ttccataccg agatttccga ctgtcgttcc
      121 caaggtggtg tcctgcacgg ataggccgat gaccaagccc agcgtgaaga tcgagttcat
      181 cagcggtttg ccgaactcgg tcgatttggc atattcgtga tcgatgtgga cggctgccgg
      241 attataggtc agcgccgaga acagaatatt gtccatgtcg gtgaccgtac gcctgatctc
      301 atgaacaaac gtttgcccga cagaaaactg ctcaaaatag agccccgcca tcttgtgtcc
      361 tcgcctcgga agtccgacga tgccgcctca agcctgtttg cggcagccgc ctcgccggct
      421 tcgttttcct acttgttgtc tctaccacat ctagcgcatg gcgtgattgt acacaagcgt
      481 acgaaatgca ggctctttga ccttcaagtc tctatccaaa gcgcaagccg cggaaagctc
      541 attgttggtc tggccgatgg actcgatcgc cgcgctattg gcgcagccca ggtgtcatgg
      601 cgccttcttc aaggcctctg gaagcttgga gcgatgaaag ccctcgaagg gagcgctgcg
      661 attgtgcgcc tcaagcttcc acgtagtgcg cgcgttcggc gtgccgccat cgacacggat
      721 gcatgagccc gtgacatagg ccgcggcagg cgacaacaag aacgtgacgg ccgcggaaat
      781 ttcagcttcc gtgccaaatc gctgcagtgg cactccagcc gtaaaatcat gcagcttctt
      841 ctgcatttca ggcgtgtagg tgtcaaaccc gctggacacg atccctcccg gagcgacagc
      901 attgacgcgc acgcctgacg cggaccattc gcacgccgcg gtttcggtaa gggtcagcat
      961 tccgccgcgc gccgcgcccg agtgggcgaa ctcgggccag ccatgccaga tgtcagcaat
     1021 gatgttgacg atcgccccgc cattagcctc catccagcgg ttgtagacct cgcgcatgaa
     1081 aatgaagccg ccggtcaggt tgctccggac caccgcctcg aagcccttgg ttgagatcgt
     1141 cttcatcggg gcccggtatt gaccgccggc gttgttcacg agcccatcga tgcggccgta
     1201 ggcgccgagc accgcgtcga tcgccgcgat caccatcgct tcgtcccgga tatcgcaaac
     1261 ctgcgtgagt atctgtccgc cgtcctcctc gatctccgcc ttcacctccg ccagtttttc
     1321 ggcggtccgg ccgaggatcg cgacgttggc gccaagggcc acgagctcgt gtgcggtgca
     1381 gcgaccgata ccgctgccgc cgcccgtgac gatgattgtc tggcctttga agagattcgg
     1441 cctgaagacc gactgatacg gcatcgtgtt ctccgaactg atcgtctcgg cggctggcgg
     1501 caaggccgca aaaactagta cactatatta tgatatggtg tattctcttg cgcaagagag
     1561 catttgcgaa tccgcgtccg gtcccgccgc cggcatctgg aacaacaacg cgactgtatg
     1621 gctatcgacg catcaccgtg acttcctgct agctggacga cggatggtgc ctttgggaac
     1681 gcacgacatg gacaagttca cgacgatcac cgtcgaacaa cgtgacgcga ttgaaatcct
     1741 ctccctcaat cggcccgatg ccctcaatgc ggtgacgccc gcgatggccg acgaactgat
     1801 cgactatttt tcggggctcc acaatcgcct gggtacgcgc atcgtcatct ttcggggcaa
     1861 tggacgggcg ttctgcgccg gcgctgagtt gggatcggac gcgttctctc cgccgggacc
     1921 aggacggccg cagcggcagc ttgacatgca gcagcgctac tcgcggatca tccgtctgat
     1981 gcgaagctgc ccgcagccga tcgtggcact ggttcaaggt gcagcctgcg gcgcagggtt
     2041 ttcgttgcta ctggcctccg atgtccgctt tgccacgcgg gctgcgcgga tgaacgccgc
     2101 ctatatacgt gtcggcgtcg gcggctgcga catgggatcc ggctatctgt tgccccggct
     2161 gatcggtctt tcggtggcgt ccgaactgtt gttgaccggg cggtttctcg aagcggatcg
     2221 ggccaaggcc gtagggctgg tcagcgacgt cgtcgacgac gataaactac tcgagaccgg
     2281 gctcgcgttc gccgccgaga tggcccgcgc ctcgccgatg gg
//
LOCUS       DASWGM010000577        20295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1287806,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000577 DASWGM010000000
VERSION     DASWGM010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20295
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287806"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /gene="shc"
                     /locus_tag="VGA15_16365"
     CDS             <1..1603
                     /gene="shc"
                     /locus_tag="VGA15_16365"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX9589305.1"
                     /translation="AIRSRGGAIHSNVFTRFMLAMFGVMTWRSVPVLPVEIMLLPMWS
                     PFHINKISYWARTTIVPLMVLAALKPRAQNVGGIGIDELFLQPPDSIGMTAKAPHQSW
                     GWFLLFRGLDSVLRVIEPLFPKKLRARAIDSAVAFIEERLNGEDGLGAIYPPMANAVM
                     MYQVLGKPADYPPRAFQRRALDRLLVIGEHEAYCQPCMSPVWDTSLTCHAMLEAAGGE
                     ALPAAKQGLDWLLPKQVLDVKGDWAVKRPDIRPGGWAFQYNNAYYPDLDDTAVVVMAM
                     DRARRASQSREYDAAIARGREWIEGMQSRDGGWAAFDVNNLEYYLNNIPFSDHGALLD
                     PPTEDVTARCVSMLAQLGETVQGSKAVADGVAYLRRTQLAEGSWYGRWGLNYIYGTWS
                     VLCALNAAGVDHQDPVIRNAADWLLSIQNKDGGWGEDATSYRLDYKGFEGAPTTASQT
                     AWALLGLMAAGEVGHPAVARGVEYLKSTQTEKGLWDEQRYTATGFPRVFYLRYHGYSK
                     FFPLWALARYRNLKSTNSRVVGVGM"
     gene            1600..2334
                     /locus_tag="VGA15_16370"
     CDS             1600..2334
                     /locus_tag="VGA15_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HEX9589306.1"
                     /translation="MNLGAVEDASAGNSIDPRPVLIVTGLVQEARIAAGPGMTVICSS
                     SDPQQLRALLTVFDPSTIRGVISFGVAGGLDPTLKSGDVVVATEVLSGDARWLAGLAL
                     NEELIASVALGRRRVVRGCLAGVEQVILARAAKAALHSKTGAAAVDMESHIAAAYAAE
                     AGLPFAALRVISDPAHRALPALAMAAIKPNGDIDLRGVLRGLARNPTTLRALVSTGID
                     FNRALKSLRGCRGFLLGGEGLVAADV"
     gene            complement(2275..3423)
                     /gene="hpnH"
                     /locus_tag="VGA15_16375"
     CDS             complement(2275..3423)
                     /gene="hpnH"
                     /locus_tag="VGA15_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX9589307.1"
                     /translation="MAIPFFKEMRIGGYLMKQKLLGRQRYPLVLMLEPLFRCNLACVG
                     CGKIDYPDAILNRRMSAQECWDAADECGAPMVAIPGGEPLIHKEIGEIVRGLVARKKF
                     VSLCTNALLLEKKLDLFEPSPYLFFSVHLDGLKEHHDKAVSQKGVFDRAVSAIKAAKA
                     KGFTVNVNATIFDGHAAEDIAKFLDFTTELGVGVSMSPGYAYERAPDQEHFLNRTKTK
                     KLFRDVFALGKGKTWNFMHSGLFLDFLAGNQTYECTPWGMPARNIFGWQKPCYLLGEG
                     YTKTFRELMETTDWETYGTGKYEKCADCMAHCGYEPTAANAAFANPLKAMWVALRGVR
                     TTGPMAPEISLDHQRPAQYIFSAEVQKRLSDIRRDEALAAKEKASTAA"
     gene            complement(3458..4384)
                     /gene="ispH"
                     /locus_tag="VGA15_16380"
                     /gene_synonym="lytB"
     CDS             complement(3458..4384)
                     /gene="ispH"
                     /locus_tag="VGA15_16380"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9589308.1"
                     /translation="MEVYLAQPRGFCAGVVRAIEIVERALQKYGPPVYVRHEIVHNKY
                     VVESLKNKGAIFVEDLSEVPPLAVTVFSAHGVARSVEEEAAVRGLPVLNATCPLVTKV
                     HNQGKRYMSKGRALILIGHAGHPEVEGTMGQVPGPVLLVQNVDDVAALTLPADKPVAY
                     ITQTTLSVDDTKDIIAALQQRFTDIQGPDIRDICYATQNRQSAVRDLSKLVDVILVVG
                     ATNSSNSNRLREIGTEVGVASYLIADGSELNPEWLKDARAVGITAGASAPEVLVDDVI
                     EALRRIGPVTVSVLPGREENIEFRLPAELTAG"
     gene            4776..7364
                     /locus_tag="VGA15_16385"
     CDS             4776..7364
                     /locus_tag="VGA15_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9589309.1"
                     /translation="MLTSIVVSVVKACTRFAPLTIVVALILAITAGYYTSRNFIINTD
                     INTLISPDLDWRKRDNQFEKAFDRDRTILAVVDAPTPELTSAAAAALEQKLSGDGRHF
                     ESVQPLGAGPFFEKNGLLFLPVAEVAQLTGQFESAAPLIEIMAGDPSIRGLTGALETG
                     LAGVKRGQVKLDSTERPFTLIAQTVENVLKNGSATFSWRELTSDKPLADSDRRSFIEF
                     KPILDFKALEPGKDATDAIRQAARDLNLAGEYGARVRLTGPVPIANEEFATVQDGAIV
                     NGIGTVLVVLLILWMALHSAKIISAVFLNLFIGLSITTAVGLWMVGSLNLLSIAFAVL
                     FVGLGVDFGIQFSVRYRSERFKSDDLRLALKKAAERSAVPLSLAAMATAAGFLSFLPT
                     DYKGISELGKIAGVGMLVAFTTSITVLPALLRLLNPPGESEPVGYAFLAPVDYFLEKH
                     RVVIIVGTLLIAVAGLPLLYFLRFDFNPINLRSPKVESIATFLDLRSDPNTGANAINV
                     MTNSDADAKKIEERLSKVPEVLRVMSLDSFVPEDQPAKLKLIGQGNKILNPALNPDSI
                     DAPPSDQENVESLKGSAENLRRTAGDAKGPGAVASRRLADALSKLADSDQATRDKTQA
                     IFVSPLKVVLDQLRNSLQAQPVGLKTLPADLVNSWKTKDGLTRVEALPRGDPNDNDTL
                     RKFAAAVLAAEPNAIGGPVSILKSGDTVVRAFIHAGITALLVISFLLWLALRRVTDVL
                     LTLVPLLVAGAVTLEICVLIGLPLNFANIVALPLLLGVGVAFKIYYVTAWRSGRTNLL
                     QSSLTRAIFFSALTTATAFGSLWLSSHPGTASMGKLLALSLVTTLAAVLLFQPALMGK
                     PRDIGE"
     gene            complement(7332..7979)
                     /locus_tag="VGA15_16390"
     CDS             complement(7332..7979)
                     /locus_tag="VGA15_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX9589310.1"
                     /translation="MLHCPRNITRTLAYSGFFLATCFTSAFAADPTGDWKVADGVANI
                     RVAECNGSMWGAVSWEKSPGGHDINNPDVAKQSRPTLGMPILIGMKKKPGVDQWEGQV
                     YNGKDGKLYSSTIKPVGSDQLEIQGCVLGFLCGGETWTRVAGPIPSSPANSMAKGGPK
                     TAGAAPPKTAASTTTATAPKTNPAPKMPGQKLATGQPADAVGDICLLPDIAGFAH"
     gene            8080..9471
                     /gene="hpnO"
                     /locus_tag="VGA15_16395"
     CDS             8080..9471
                     /gene="hpnO"
                     /locus_tag="VGA15_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobacteriohopanetriol synthase HpnO"
                     /protein_id="HEX9589311.1"
                     /translation="MLYPNLDVSEMFVDRQSQRSSMHARHLNEQLVRVLKTIGYDVGF
                     QKGQGQYLFDRDGARYLDLLSGFGVFAIGRNHPALRQALKSVLDSDLPNLVQLDVSTL
                     AGVLAERLLEFVPYLDKVFFANSGAECVEAAIKFARGATGRPGIVYCAHGYHGLTYGA
                     LSLTDDPNFRGGFEPLLPGCTSIPFNDLAALEQALSSRQVAAFIVEPIQGKGVNMPTD
                     EFLPGAAALCRKYGTLMVADEIQTGMGRTGRFLAVEHWNVEPDIVLLSKALSGGHVPV
                     GALLTRKSIFEKIFNQMDRAVVHGSTFAKNDLAMAAGIATLEVIESEKLIDAAAKRGA
                     ELRLALTRMVPGYELMKEVRGKGLMIGVEFGPPKSMRLKASWNLLETANKGLFCQLIT
                     VPLFKDHKILTQVSGHGSHTIKLLPSLTITEEDCNWIETAFDTVIASSHKVPGAIWSL
                     GKTLVDNAVRKSA"
     gene            9617..10225
                     /locus_tag="VGA15_16400"
     CDS             9617..10225
                     /locus_tag="VGA15_16400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9589312.1"
                     /translation="MAEWFAELDPAVSAKIVRALARIEQGNLSNVKRVGEGVLEYRID
                     FGPGYRIYFGRDGETIIILLTGGTKKRQQAISMRRMLTGRNTSGASAREAERRRAMAR
                     TKSFKELVQGRVKADKKFAEALLREGIDAMLSGDVDTGKAILRDYIKATVGFEELGEA
                     TGAPPKSLIRMFGPRGNPQAKNLFSVLGYLQKRAGLSLHVAG"
     gene            complement(10419..11180)
                     /locus_tag="VGA15_16405"
     CDS             complement(10419..11180)
                     /locus_tag="VGA15_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX9589313.1"
                     /translation="MTCDEAEILLHALIDGELDAGHTRAVEEHIAGCPRCAAELAAYR
                     EMSRATASANLRYTAPPALRRRIEASLPQARMPNRRAVLRGFAMGSAVSAIAASGLFA
                     IVVRNDDEARIESEVVSAHLRSLQAGHLTDVISTDQHTVKPWFNGKLDVAPPVVDLTA
                     QGFTLIGGRLDYVDARPIGAIVYRRRAHVINLFVAQTASTERHAAKIDTIQGFNIRRW
                     SERGLNYWAVSDLAADELAEFGDKFETAMRTGTAG"
     gene            complement(11194..11742)
                     /locus_tag="VGA15_16410"
     CDS             complement(11194..11742)
                     /locus_tag="VGA15_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX9589314.1"
                     /translation="MPVNAANDDPERARRFRDAALPHLDDVYTLARYLLRDAADAEDA
                     VQECYLRALKHFDSYRGPAMKPWLFAILRNVCRAEFARRASSPTAPIEDVPEDAQQTP
                     LWHDAPETPEAQMLHRWDASTIRRLVEALAEPFRETFVLREIHELSYREIADVVGAPV
                     GTVMSRLARARAMLRSAWMAEE"
     gene            complement(11834..12169)
                     /locus_tag="VGA15_16415"
     CDS             complement(11834..12169)
                     /locus_tag="VGA15_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEX9589315.1"
                     /translation="MTPVTLRSHGLRLAMAAAISLCLGGAHAEETTTNVMIDNFTFRP
                     AELTVKVGTTVTWKNRDDIPHTVVSAGKLRSKTLDTDDSFTFTFTAAGDYKYFCSLHP
                     HMTGMIKVE"
     gene            complement(12183..13145)
                     /locus_tag="VGA15_16420"
     CDS             complement(12183..13145)
                     /locus_tag="VGA15_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9589316.1"
                     /translation="MSGFDHDHDNEGPNEGPGVSRRRVLECMTWAGTGVLWTISGGVP
                     RSLGLVGSAQAAESAGMTFLQISDSHVGFDKPANPNALGTLEEAINKINAMPAKPSFM
                     IHTGDISHLSKVVEFDNADRIISQAKLDVHYVPGEHDFLDEENKLYKERYGRGAKGAG
                     WYSFDADGVHFIGLVNVVNLKAGGLGNLGSEQLEWLEDDLKGKSKSTPIVVFAHIPLW
                     TVYPEWGWGTEDGARALDYLKGFGSVTVLNGHIHQVMQKVEGNVTFHTARSTAFPQPA
                     PGTAPSPGPMKVADDKLRGLLGVASINFKRGDQNLAIIDTPLQG"
     gene            13385..13594
                     /locus_tag="VGA15_16425"
     CDS             13385..13594
                     /locus_tag="VGA15_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589317.1"
                     /translation="MADHAGVEHHHKAAEHHEQAAKHHREAARHHEAGDHEKAAHHAH
                     SAHGHASHAEEHHAEASRHHAEHHG"
     gene            complement(13811..15427)
                     /locus_tag="VGA15_16430"
     CDS             complement(13811..15427)
                     /locus_tag="VGA15_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9589318.1"
                     /translation="MKQLEGDFDYIVVGAGTAGCIVANRLSADPKNRVLILEAGGSDN
                     WIWFHIPVGYLFAIGNPRSDWMFRTEPEAGLNGRSLAYPRGKVIGGSSAINAMISMRG
                     QAADYDHWRQLGLSGWGYDDVLPAFRRLEDHFLGESEHHGAGGGWRIEAPRLSWAILD
                     AVGDAAEEMGIRKIPDFNTGDNEGVGYFHVNQKRGRRLSSARGFLKPALQRRNLRLEK
                     DVLVDRLIVEHGRAVGVRFIQGGETVEARAKAEVILCAGSIGSTQVLHRSGIGPAEWL
                     APLGIETVLDRQGVGRNLQDHLQQRAIYQVDGVRTLNETYYSLVRRGLMGLDYAFRRR
                     GPLTMAPSQLGIFTRSDPRRDRANIQFHVQPLSLDKFGEPLHRFPAITVSACNLQPTS
                     RGTVRIRSAKPDEAPSIAPNYLTTSEDREVAADAIRTTRRLMKQPALAPYHPQEFLPG
                     PSVGDDDAALAKAAGDIGTTIFHPVGTAKMGPASDPTAVVDERLRFIGLAGLRVVDAS
                     VMPTITSGNTNTPTAMIAEKGAAMILEDSR"
     gene            15556..16728
                     /locus_tag="VGA15_16435"
     CDS             15556..16728
                     /locus_tag="VGA15_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX9589319.1"
                     /translation="MPIVNRVADLQPDIQAWRQDIHAHPELLYDVHRTAAFVADRLRE
                     FGCDEVATGLGRTGVVGVIKGRKPAGKSDIRVIGLRADMDALPIEEETHLAYASRTPG
                     KMHACGHDGHTAMLLGAARYLAETRNFAGDAVVIFQPAEEGGAGAAAMIEDGLMDRFG
                     IDQVYGMHNGPGIPIGSFAIRSGPIMASTDSIDIRIEGLGGHAARPHKCIDSVLVGAQ
                     LITALQSIVSRSVNPLDSAVISMCEFHAGVARNVIPQTAILRGTVRTLTVEVRELVEK
                     RVREVVAGVAQITGAKIDFVYERGYPVVVNHAAQTDVATQVAKEIAGDANVHETHPFM
                     GAEDFAYMLESRPGAFIFCGNGDSAGLHHPAYNFDDNAIVFGTSYWIRLVETALAA"
     gene            complement(17053..18681)
                     /locus_tag="VGA15_16440"
     CDS             complement(17053..18681)
                     /locus_tag="VGA15_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017968069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX9589320.1"
                     /translation="MTDFALNQTTDEGAPVQSAARPNLDKGFNPLTLIMFFGILAAGL
                     LFVAYSIYADVDATGVKVTTYLPYLLLLVALLIALGFEFVNGFHDTANAVATVIYTHS
                     LPAEFAVMWSGFFNFLGVLLSSGAVAFGIVSLLPVELILQVGSSAGFAMVFALLIAAI
                     IWNLGTWYFGLPASSSHTLIGSIIGVGIANALMRGRDGTSGVDWSKATEIGYALLLSP
                     LVGFIFAALLLLLLKVIVRNPALYAAPEGNKAPPLWIRGLLILTCTGVSFAHGSNDGQ
                     KGMGLIMLILIGTVPTAYALNRALPESQVALFQQTSAAASTVIAAKGAGHSIIGDPRP
                     AVTQYVAQHTLSEGTYPSLAVLVKDVGDQVQKYGSLSKVPSEAVGNTRNDVYLTSEAI
                     RFLMKDKQNDLNKEEIGILNAYKSSLDAATKFIPRWVKIAVAIALGLGTMIGWKRIVI
                     TVGEKIGKSHLTYAQGASAELVAAATIGAADMFGLPVSTTHVLSSGVAGAMAANGSGL
                     QVATIRNMVMAWVLTLPVAIVLSGSLYVIFSRLF"
     gene            18921..19418
                     /locus_tag="VGA15_16445"
     CDS             18921..19418
                     /locus_tag="VGA15_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX9589321.1"
                     /translation="MTAKDKHLNDLFLDTLKDIYYAEKQILRALPKMAKAASSDKLRA
                     AFEKHHGETEGQVERLEQIFELLDKPARGKTCDAIQGILDEGKEIMDEYKGSEALDAG
                     MLAAAQAVEHYEISRYGTLKQWATQLGMKDAVRLLDQTLQEEKKTDESLTTLAEAAVN
                     LAAAA"
     gene            complement(19566..19823)
                     /locus_tag="VGA15_16450"
     CDS             complement(19566..19823)
                     /locus_tag="VGA15_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589322.1"
                     /translation="MASLFTSEIKGIQAMDVQRKLAIPADAAKYDASCASSGIETQHS
                     PEWRAAVAKIGARGLVSQKARTSHSSPLLLWERVASRSEDG"
     gene            20014..>20295
                     /locus_tag="VGA15_16455"
     CDS             20014..>20295
                     /locus_tag="VGA15_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HEX9589323.1"
                     /translation="MILSPNRSHFGGSCATVRTNTLATLRGSIERIEAHGDTYRPDRV
                     ALGHADADATLQGGLALGAMHEVFAEGRHSAAATGFVTGLMGRAAARRPL"
BASE COUNT         3811 a         6169 c         6348 g         3967 t
ORIGIN      
        1 ggcgatccgc tcgcgcggcg gcgccatcca cagcaacgtt tttacccgct tcatgctggc
       61 gatgttcggg gtgatgacct ggcggagcgt gccggtattg ccggtcgaga tcatgctgct
      121 gccgatgtgg tcgccgtttc atatcaacaa gatttcgtac tgggcgcgga ccacgatcgt
      181 gccgctgatg gtcttggcgg cgctgaaacc gcgcgcgcaa aatgtcggcg gcatcggcat
      241 cgacgagctg ttcctgcagc cgccggattc gatcgggatg acggccaagg cgccgcatca
      301 gagctggggc tggtttctgt tgttccgcgg actggacagc gtgttgcggg tgatcgaacc
      361 cctgtttccg aagaagctgc gtgcccgcgc gatcgacagc gcggtcgcct tcatcgagga
      421 gcggttgaac ggcgaagacg gcctgggcgc gatctatccg ccaatggcca atgccgtgat
      481 gatgtatcag gtgctgggta agcccgccga ttatccgccc cgcgccttcc aacgaagggc
      541 gctcgacagg cttctcgtga tcggcgagca cgaggcctat tgccagccct gcatgtctcc
      601 ggtgtgggac acctcgctga cctgtcatgc gatgctcgaa gcagctggcg gggaggcgct
      661 gcccgcggcg aaacagggcc tcgattggct gctgccgaag caggtgctgg atgtcaaggg
      721 cgactgggcc gtcaagcgcc ccgacattcg ccccggcggt tgggcgttcc agtacaacaa
      781 cgcctattat cccgatctcg acgatactgc cgtggtggtg atggcgatgg atcgcgcgcg
      841 gcgcgccagc caaagccggg aatacgatgc tgcgatcgcg cgcggccgcg aatggatcga
      901 gggcatgcag agccgcgatg gcggctgggc cgccttcgat gtcaacaatc tcgaatatta
      961 tctgaacaac attccgttct ccgatcacgg cgcgctgctc gatccgccga ccgaggacgt
     1021 caccgcccgc tgcgtctcga tgctggctca actcggtgag accgtccaag gcagcaaggc
     1081 ggttgcggac ggggtcgcgt atctgcgccg gacccagctc gcggaagggt cgtggtacgg
     1141 ccgctggggc ctgaactaca tttacggaac ctggtccgtg ctgtgcgcgc ttaatgccgc
     1201 gggggtggac caccaggacc ccgtgatccg caacgccgcc gactggctgc tgtcgatcca
     1261 gaacaaggac ggcggctggg gcgaggatgc caccagctac cggctcgatt acaagggctt
     1321 tgagggggcc cccaccaccg cctcacaaac ggcatgggcc ttgcttggcc tgatggcggc
     1381 aggcgaggtc gggcatcccg cggtcgcgcg gggggtggag tacctaaaaa gcacacagac
     1441 cgaaaaaggc ctgtgggacg agcagcgcta cacggcaacg ggctttccgc gagtgtttta
     1501 tttgcgttat catggctatt ctaagttctt tccgctctgg gcgctggcgc ggtatcgaaa
     1561 tttgaagagc accaacagta gagtggtagg ggtcggaatg tgaatttggg ggcggtggaa
     1621 gacgcgagcg cgggcaattc gattgatccg cggccggtgt tgattgtgac cggtctggtg
     1681 caggaagccc gcattgcggc agggcccggc atgaccgtca tctgcagcag cagtgacccg
     1741 cagcaattgc gcgcactcct gacggtgttt gacccgtcga cgatcagggg cgtcatcagc
     1801 ttcggcgtcg cgggtggact tgatcctaca ttgaaatcgg gcgacgtcgt ggtggcgacc
     1861 gaagttcttt ccggtgacgc ccgctggctg gccggtctgg cgctcaatga agaactgatc
     1921 gccagcgtcg cattgggacg ccggcgcgtc gtcagggggt gtcttgcggg cgtcgagcag
     1981 gtgatcctcg cccgcgccgc aaaggccgcg ctgcattcga agaccggcgc cgccgccgtc
     2041 gatatggaga gccatatcgc cgccgcctac gcagccgagg cgggccttcc gttcgcagcc
     2101 ttgcgcgtga tcagcgaccc cgcccaccgc gccttgccgg cgctggcgat ggccgccatc
     2161 aagccgaacg gagacatcga cctgcgcggg gtgctgcgcg gcctggcgcg caatccaacg
     2221 acgctgcgcg cgctggtctc gaccgggatc gatttcaatc gcgcgctgaa gagcctacgc
     2281 ggctgtcgag gctttctcct tggcggcgag ggcctcgtcg cggcggatgt ctgacaggcg
     2341 tttctgcact tccgccgaga agatgtactg cgccggacgc tggtgatcga gactgatctc
     2401 gggcgccatc ggaccggtgg tgcggacgcc gcgcagcgcc acccacatcg ccttcaatgg
     2461 attggcaaaa gccgcattcg ccgcggtcgg ctcgtagccg caatgcgcca tgcagtcggc
     2521 gcacttctcg tacttgccgg tgccgtaggt ctcccagtcg gtggtctcca tcagctccct
     2581 gaaggtcttg gtgtaacctt cgccgagcag atagcagggc ttctgccagc cgaaaatgtt
     2641 gcgtgccggc atgccccacg gggtgcattc gtaagtctga ttgcccgcaa gaaaatccag
     2701 gaacaggccg gaatgcatga aattccacgt cttgcccttg ccgagcgcga acacgtcgcg
     2761 gaacagcttc ttggtcttgg tgcggttgag gaagtgctcc tggtcggggg cgcgctcata
     2821 agcgtagccc ggcgacattg aaacgccgac cccgagttcc gtcgtgaagt cgaggaattt
     2881 ggcgatgtcc tcggcggcgt gtccgtcgaa gatcgtggca ttgacgttga cggtaaaacc
     2941 cttcgccttg gccgccttga tcgccgagac cgcgcgatcg aacacgcctt tctgcgagac
     3001 ggccttgtcg tggtgctctt tcaagccgtc cagatgcacc gagaaaaaca gatatggcga
     3061 cggctcgaac aggtcgagct tcttctccag cagcagcgcg ttggtgcata gcgacacgaa
     3121 tttcttccgc gccaccaggc cgcgcacgat ctcgccgatt tccttgtgga tcagcggctc
     3181 gccgccgggg atagccacca tcggcgcgcc gcactcgtcg gcggcatccc agcattcctg
     3241 tgccgacatg cggcgattga ggatcgcatc gggatagtcg atcttgccgc agccgacgca
     3301 ggccagattg cagcgaaaca acggctcgag catcagcacc agcggatagc gctgtcggcc
     3361 aagcaatttc tgcttcatca ggtagccgcc gatgcgcatt tccttgaaga acggtattgc
     3421 cattgcaggt tttctttctg gactcaagtg aagtggatca acccgcggtc agttcggccg
     3481 ggagccggaa ttcgatattt tcttcccgac ccggaaggac cgacaccgtc accggtccga
     3541 tccgtctcag ggcctcgatc acgtcgtcga caagaacctc gggtgccgag gcgccggcgg
     3601 tgatgccgac cgccctggcg tccttcagcc attccgggtt cagctcgctt ccgtcggcaa
     3661 tgagataact cgcgaccccg acttcggtgc cgatttcgcg cagcctgttt gagttggaac
     3721 tgttggtggc gcccaccacc aggataacat ccacgagttt gctcagatcc cttaccgcag
     3781 attggcggtt ctgtgtcgca tagcagatat cccggatgtc ggggccttga atatctgtaa
     3841 accgctgctg aagggctgca attatgtctt ttgtgtcatc cacgctgagg gtggtctggg
     3901 tgatataggc caccggcttg tcggccggca gcgtcaatgc cgccacatcg tcgacatttt
     3961 gcaccagaag taccggtccg ggaacctgac ccatggttcc ctcgacctcg gggtgaccgg
     4021 cgtgaccgat caggatcaac gcccggcctt tggacatgta gcgcttgccc tggttatgga
     4081 ccttggtcac cagtgggcag gtggcattga ggaccggcag gccgcggacg gcggcttcct
     4141 cctccacgct cctggccacg ccgtgggcgc tgaatacggt gaccgcgagc ggcggcacct
     4201 ccgacagatc ctcaacgaag atcgcgcctt tgttcttcag gctttcaacc acgtatttat
     4261 tgtggacgat ctcatgccgg acatagaccg gcggaccata tttctgaagc gcgcgctcga
     4321 cgatctcgat tgcacgcacc acgcccgcac aaaatccgcg gggctgcgca agatatactt
     4381 ccatgggact tccgttacgc tagttgcacc aaactcatac agtatctccg cgcggctttg
     4441 gtctgtgatc tatgcaacat ccgaaccatg accggttttc gtatcccttg cgccacaggg
     4501 cggcaggccc gaaccggtat tataatacag ttaaccacaa aaccgcggca aaccaagcca
     4561 aaaccctcat ttgttcgtta cgcgaccggc gccggtcccg ataatgtgag ccgctccagc
     4621 gcagtttcgg cattttttgc gctacttgtt ggtgttcctg aggaaagaac ccaaaacaac
     4681 attagatcgg ttgacggaac tccggtagac agcgtgcgtt tccggcaagc tctccgcagt
     4741 tcccggcaag ctcaataaca tcagaaagag aaaatgtgct gacaagtatt gttgtctcag
     4801 ttgtgaaagc ttgcacgcgg ttcgcgcctc tcacgatcgt ggtcgcactt atcctcgcga
     4861 ttacggcggg ctactacacg tcccggaact tcatcatcaa caccgacatc aataccctga
     4921 tctcgcccga tctcgactgg cgcaagcgcg acaatcagtt cgagaaggcg ttcgatcggg
     4981 atcgaacgat tctggccgtg gtggacgcgc cgacgccgga gctgaccagc gctgcggcgg
     5041 ccgcattgga gcagaaattg tctggtgacg gcaggcattt cgaatcggtc cagccgctcg
     5101 gcgccggacc gttctttgaa aagaacgggc tgttgttcct gccggtggcg gaagtagccc
     5161 agttgaccgg ccagttcgaa tccgcagccc cgctgatcga gatcatggct ggcgatccca
     5221 gcatacgggg actgaccggc gcgctggaaa ccgggctcgc cggcgtcaag cgcggacagg
     5281 tcaaactcga cagcaccgag cggccattca cgctgatcgc ccagaccgtg gagaacgtgc
     5341 tcaagaacgg ctcggcgacc ttctcctggc gcgaactcac cagcgacaag cctttggccg
     5401 actccgatcg gcgctcgttc atcgagttca agccgatcct cgacttcaag gcgctggagc
     5461 ctggcaagga tgcgaccgat gccatcaggc aggccgcccg cgatctcaat ctcgcaggcg
     5521 aatatggcgc gcgggtcagg ctgaccggac cggttccgat tgccaacgag gaattcgcca
     5581 ccgtccagga tggcgccatc gtcaacggga tcggcaccgt gctcgtggtg ctgttgattc
     5641 tctggatggc gctgcattcc gccaagatca tctccgccgt gttcctgaac ctctttatcg
     5701 gcctttccat caccaccgcc gttgggctgt ggatggtggg ttcgctgaac ctgctctcga
     5761 tcgcattcgc cgttctgttc gtcggactgg gcgtcgactt cggcatccag ttcagcgtcc
     5821 gctaccggtc ggagcgcttc aaaagcgacg acctgcggct ggcgctgaaa aaggccgcgg
     5881 aacgctccgc ggtcccgctt tcgctcgctg cgatggccac cgccgccggc tttctctcgt
     5941 tcctgccaac cgactacaag ggcatttcgg aactcggcaa gatcgcgggc gtcgggatgc
     6001 tggttgcctt tacgaccagc atcacggtgt tgccggcgtt gctgaggctg ctcaatcctc
     6061 ccggggaatc ggaaccggtc ggctatgcgt tcctcgcgcc ggtcgattat ttcctcgaaa
     6121 agcatcgcgt cgtcatcatc gtcggcacgc ttttgatcgc ggtcgccggt ctgccgctgc
     6181 tttatttcct gaggttcgat ttcaacccga tcaacctgcg aagtcccaag gtggaatcga
     6241 tcgcgacttt tctcgatctg cggagcgacc cgaacaccgg cgccaacgcc atcaatgtga
     6301 tgacgaactc ggacgccgac gccaagaaga tcgaagaacg gctgtccaag gtgccggaag
     6361 tgctccgcgt gatgtcgctg gatagttttg tgccggaaga ccagccggca aaactgaagc
     6421 tgatcgggca aggcaacaag attcttaatc ccgcgctcaa cccggactcg atcgatgcgc
     6481 cgccgtccga tcaggagaat gtcgagtcct tgaagggatc ggcggaaaac ctgcgcagga
     6541 ccgccggcga tgccaagggt cccggcgccg tggcctcgcg gcggcttgcc gacgcgctgt
     6601 cgaagctcgc cgattcggat caggccacgc gggacaagac ccaggccatc ttcgtttcgc
     6661 cattgaaggt ggtgctcgac cagctcagga actcgctgca ggcccagccg gtcggcctga
     6721 agacgctgcc ggccgatctg gtgaattcgt ggaagaccaa ggatggcctg acccgcgtcg
     6781 aggccctgcc gcgcggcgat cccaacgata acgacacgct gcgcaagttc gcagcggcgg
     6841 ttctggccgc cgagccgaac gcgatcggcg ggccggtatc gattctcaag tccggtgata
     6901 ccgtcgtcag ggccttcatc cacgctggca ttaccgcgct gctcgtgatc agctttctgc
     6961 tttggctggc gctgcggcga gtaaccgatg tgctgctgac gctggtgccg ctgttggtcg
     7021 ccggcgcggt gacgctggaa atctgcgtgc tgatcgggct gccgctcaac ttcgccaata
     7081 tcgtggcgct gccgttgctg ctcggcgtcg gcgtcgcgtt caagatatat tatgtcacgg
     7141 cgtggcggtc gggcagaacc aatctgctgc agtcgagcct gacgcgcgcg atatttttca
     7201 gcgcgctgac gacggccacc gcgttcggaa gcttgtggct atcgagtcat ccgggcaccg
     7261 ccagcatggg caagctgctg gcgctctccc tggtcacgac gctggccgcg gtgctgttgt
     7321 tccagcccgc gctaatgggc aaaccccgcg atatcgggga gtagacagat atcgccaacc
     7381 gcatcggccg gctgcccagt cgccagtttt tgaccgggca tcttgggagc gggattggtc
     7441 ttcggcgcgg tggctgttgt tgtcgatgcg gcggttttgg gcggagcagc acccgcggtc
     7501 ttcggcccgc ccttggccat gctgttggcc gggctactgg gaatcgggcc cgcgacgcga
     7561 gtccaggttt cgccgccgca gaggaagccc aacacgcaac cttgaatttc aagttggtcg
     7621 gaaccgaccg gcttgatggt cgaactgtat aattttccgt ccttgccgtt atagacttgg
     7681 ccttcccatt gatcgacgcc aggcttcttc ttcatgccga tcaggatcgg cattcccagg
     7741 gtcggcctgc tttgttttgc gacatccgga ttgtttatgt cgtggccgcc cggcgacttc
     7801 tcccacgaca ccgcgcccca catgcttcca ttgcattccg cgacgcggat gttggcgacg
     7861 ccgtccgcca ccttccagtc gcctgtcgga tccgccgcaa aggctgatgt aaagcaagtc
     7921 gccaaaaaaa atccggaata tgctagtgta cgcgtgatgt ttcgagggca gtgcaacatg
     7981 gtttagacct gtgcttaagt agatgatccg agtgagggca aaacgccgca ttgtgcgttt
     8041 tcagttgacg gaatggccac caaccgaagt atgtaaccga tgctatatcc caatctagac
     8101 gtttccgaga tgtttgtgga ccgccagtcg cagcgcagtt ccatgcatgc gcgccactta
     8161 aatgagcaac tggtgcgggt cctcaagaca attggttacg atgtcggctt ccagaagggt
     8221 caaggccagt atctgttcga tcgcgacggc gcgcgctatc tcgatctatt gagcggtttt
     8281 ggcgtttttg cgattggccg gaatcacccg gcgctgcgcc aggccttgaa gagcgtcctc
     8341 gacagcgatc tgcccaacct cgtgcaactc gatgtttcga cgcttgcggg cgtgctggcc
     8401 gaacgcctgc tggaatttgt tccatatctg gacaaggtat tttttgccaa ttccggagca
     8461 gaatgcgtcg aagctgcgat caaattcgcc cgcggcgcga cgggccggcc gggcatcgtg
     8521 tactgcgccc atggttacca cggattgacc tatggcgcgc tgtcgctgac cgacgacccg
     8581 aacttccgcg gcgggttcga gccgttgctg cccggatgca cctctatacc cttcaacgat
     8641 ctcgccgctc tggagcaggc gctgtcgtcg cgtcaggttg ccgcctttat cgtcgagccg
     8701 atccagggca aaggcgtcaa tatgcccacg gatgagtttc tgccgggcgc cgcagcgctc
     8761 tgccgcaagt acggcacctt gatggtcgcc gacgagatcc agaccggaat gggtcgaacc
     8821 gggcggttcc tcgcggtcga gcactggaat gtcgagcccg acatcgttct gctctcgaag
     8881 gcgctgtccg gcggacatgt tccggtcggt gcgttgctta cccgcaagag tatcttcgag
     8941 aagatcttca atcaaatgga tcgcgcagtt gtgcacggat cgacattcgc gaaaaacgat
     9001 ttggcgatgg ccgccggaat tgcgacgctc gaagtgatcg agtccgaaaa attgatcgat
     9061 gcggccgcca agcgcggcgc cgaacttcgg ctcgcgctga cccggatggt tcccggttat
     9121 gaattgatga aagaggttcg cggcaagggg ctgatgatcg gcgtggagtt cggtccgccg
     9181 aagtcgatgc ggctgaaggc gtcgtggaat ctgctcgaga ccgccaacaa gggtttgttc
     9241 tgtcagctca tcacggtgcc gctgttcaag gatcacaaga tcctcacgca agtctccggc
     9301 cacggcagcc acaccatcaa gctgttgccg tcgctgacga tcaccgaaga ggactgcaac
     9361 tggatcgaaa ccgcgttcga taccgtcatc gcctccagcc acaaggtccc cggcgcgatc
     9421 tggtcgctcg gcaagacgct ggtcgacaac gccgtgcgca aatcggcgtg atcccttact
     9481 ccaaagactg tccttacgta gggcgcaata gcgaagctta ttgccgtctt tgcccttgaa
     9541 agcgcggtaa cacatatgtt acccctcttt cgtgctcgaa atccgctatt acgttgccgc
     9601 gagcggtgag ggtcccttgg ccgaatggtt cgcggaactg gacccggcag tcagcgccaa
     9661 aattgtccgt gcgctcgccc gcatcgagca aggcaatctc tccaatgtaa aaagggtggg
     9721 agaaggtgtg ctggaatatc ggatcgattt tggtcctggt tatcggattt atttcgggcg
     9781 ggatggcgag acgatcatca tcctgctgac cggcggaacc aagaagcggc agcaggcgat
     9841 atcgatgcgg cgcatgctta ctggcaggaa tacaagcgga gcaagcgcgc gcgaggctga
     9901 acggagacgt gcgatggcaa gaacaaagag cttcaaggaa ttggtgcagg gtcgggtcaa
     9961 ggccgacaag aaatttgcag aggcccttct tcgcgaaggc atcgatgcga tgctgagcgg
    10021 cgatgtcgat accggcaagg cgatcctgcg cgattacatc aaggcgaccg tcggtttcga
    10081 agaactgggc gaagcaaccg gcgcgccgcc aaagagcctg atccgcatgt tcgggccgcg
    10141 cggtaatccg caagccaaaa acctcttcag cgtgcttggc tacttgcaaa agcgcgcggg
    10201 cctcagcctg cacgtcgctg gctaggcgat cgcgtcccgt ctctgcagtc gtctggcacc
    10261 aaatgacgaa attgcggtga cagtgcattt aactggattg acggactttt cggcggcttg
    10321 atcgcccgcc ttgccgatcg cacttatttt cgcggggacg accttgattg ccccaactcc
    10381 gtcgcgaatt ctgttggctc gcactgttgg cccaaatcct agcccgccgt cccggtccgc
    10441 atcgcggttt cgaatttatc gccgaactcg gcgagttcgt cggcggcgag atctgagacc
    10501 gcccagtagt tcaatccccg ctcgctccag cgtctgatat tgaatccctg gatggtgtcg
    10561 atcttggccg cgtggcgttc ggtgctggcg gtctgggcca cgaacagatt gatcacatgc
    10621 gcgcgccgac ggtagacgat ggcgccgatc gggcgggcat cgacataatc gagccggccg
    10681 ccgatcagcg taaaaccctg cgcggtcagg tcgaccaccg gcggcgccac gtccagtttg
    10741 ccgttgaacc acggcttcac cgtatgctgg tcggtcgaga tcacatcggt gagatgtccg
    10801 gcctgcagcg agcgcagatg cgccgagacc acttcggact cgatgcgcgc ctcgtcatcg
    10861 ttgcgcacga cgatggcgaa caggccggac gctgcgatgg ccgatactgc cgatcccatc
    10921 gcaaagcccc tgagcaccgc gcgccggttc ggcatccgcg cttgcggcag cgatgcctcg
    10981 atgcgccggc gaagcgcagg cggagccgta taacgcagat tggcgcttgc cgtggccctg
    11041 ctcatctcgc ggtaggccgc aagctccgcg gcgcagcgcg ggcagcctgc gatatgctct
    11101 tcgacggcgc gcgtgtgccc ggcgtcgagt tcgccgtcga tcagcgcgtg aagaagaatt
    11161 tcagcctcat cgcaggtcat ttcgggtact ccactactct tccgccatcc aggctgaccg
    11221 cagcatggcg cgggcgcgcg caaggcgcga cattacggtg ccaactggcg cgccaacgac
    11281 gtcggcgatt tctcgatagg atagttcgtg aatctcccgc agcacgaagg tctctcgaaa
    11341 cggttcggcc agtgcctcca caagcctgcg gatcgtcgag gcgtcccatc ggtgtaacat
    11401 ctgtgcctcc ggcgtttccg gcgcgtcgtg ccagagcggc gtttgctgcg cgtcttctgg
    11461 cacatcctcg atcggggcgg tcggcgaact tgcgcgccgc gcaaattcgg cgcgacagac
    11521 gttgcgcagg atggcgaaca gccacggctt catcgccggc ccgcgatagc tgtcgaaatg
    11581 cttcaacgcc cgcagatagc attcctgcac cgcgtcctcg gcgtcggcgg cgtcgcgcag
    11641 caggtagcgc gccagcgtat agacatcgtc gagatgcggc agcgcggcgt cgcgaaaacg
    11701 ccgtgccctt tccggatcgt cgttcgcagc gttgaccggc atcgcgcttt gcccggcgtt
    11761 ggtagaaatg acgggtgccc ggccggcggg aatccaccgg ccgggcattg ctgtgatgcc
    11821 ttgatttgga ccgctactca accttgatca tccccgtcat gtgcgggtgc agcgaacaaa
    11881 agtacttgta atcgcccgcc gccgtgaacg tgaatgtaaa gctgtcgtcg gtatccagcg
    11941 tcttcgatct gagcttgccc gctgacacca ccgtgtgcgg gatgtcatcg cgatttttcc
    12001 aggtcacggt ggtaccgacc ttcaccgtca gctctgccgg ccgaaaggtg aaattgtcga
    12061 tcatcacgtt tgtcgtcgtc tcctcggcat gagcgccgcc gaggcacaag gatattgccg
    12121 cagccatggc caagcgaagg ccatggctgc gcagagttac tggcgtcatt acgaatttcc
    12181 ttttagccct gcagtggcgt gtcgatgatc gcgaggtttt gatcgccacg cttgaagttg
    12241 atgctggcaa cgccgagcag gccgcgaagc ttgtcgtcgg cgaccttcat cggcccaggc
    12301 gaaggggcgg tgcccggcgc cggctgcggg aacgcggtcg agcgcgcggt gtggaaggtg
    12361 acgttgccct cgaccttctg catcacctgg tggatgtggc cgttcaatac ggttaccgag
    12421 ccgaagccct tgaggtaatc cagcgcacgg gcgccatcct cggtgcccca gccccattcc
    12481 ggatagaccg tccacagcgg aatatgggcg aatacgacga tcggcgtgga tttcgatttt
    12541 cccttgagat catcctcgag ccattcaagc tgctcgctgc cgagattgcc gaggccgccg
    12601 gccttcagat tgacgacgtt gacgagacca atgaagtgga cgccgtcggc gtcgaacgag
    12661 taccagccgg cacccttggc gccgcgtcca taacgctcct tgtagagctt gttctcttcg
    12721 tcgaggaaat cgtgctcgcc cggcacgtag tgcacatcaa gcttggcctg cgagatgatg
    12781 cggtcggcgt tgtcgaattc gaccaccttg gacagatggc tgatatcgcc ggtgtgaatc
    12841 atgaaggacg gttttgccgg catcgcgttg atcttgttga tggcctcctc gagcgtgcca
    12901 agcgcgttgg gattggccgg cttgtcgaaa ccgacatggc tgtcgctgat ctgcaggaac
    12961 gtcatgccgg ccgattcggc ggcctgcgcg gaaccgacca ggccgagcga acggggcacg
    13021 ccgcccgaga tggtccagag cacgccggtg ccggcccagg tcatgcattc cagcacccgc
    13081 cggcggctga cgcccgggcc ttcattggga ccttcgttat cgtggtcgtg atcgaatccg
    13141 ctcatcgcca atctccatgg acccttgatt gggttgtggg gactggatcg ggcgagggcg
    13201 gggtttattc ccggaacttt cggcggctgg cggctgcgcg ataacgtttt cgtgattagg
    13261 gttgccgctg tgcccggctt tggcgggatg tcacccgcaa caaacctgtc gtgcggttgt
    13321 cagcatattg tcaggcgact tgcgtagatc ttgctgacgt ttcgcactga accgggagag
    13381 aaatatggct gaccacgcag gcgtcgagca ccatcacaag gccgccgaac accacgagca
    13441 agcagcgaag caccaccgcg aagccgccag gcaccatgag gccggcgatc acgagaaggc
    13501 agcgcaccac gcccatagcg cccacgggca tgccagccac gccgaggaac atcatgccga
    13561 ggccagccgc catcacgcgg agcatcacgg ttaatactgg agcatgaccc ggaaaagcgg
    13621 gaaccggttt tccctcggga caaacgcgaa gcatttgccc ggaggtcatg ctcaaacaaa
    13681 gagatgagat catgatccga tcggatcatg atctctgtca cggccaacaa gaacaacaat
    13741 aagaagaagt cataagcgtt gcgtttgcgt tttgccgtcc ctttccgagg gacggcgttt
    13801 ttttgaatcg ctaccgcgaa tcctcaagga tcatcgcggc gcctttctcg gcgatcatcg
    13861 cggtgggggt gttggtattc cccgaagtga tcgtcggcat caccgaggca tcgaccacgc
    13921 gaagcccggc gaggccaata aatcgtaagc gttcatcgac gaccgccgtc ggatcgctcg
    13981 ccggacccat cttggcggtt cccacgggat gaaagattgt ggtgccgata tcacccgcgg
    14041 ccttcgccag cgccgcatcg tcgtcgccga ccgatgggcc cggcaggaat tcctgcgggt
    14101 gatagggcgc gagcgcgggc tgtttcatca ggcgacgggt ggtgcggatg gcgtcggcgg
    14161 cgacctcgcg gtcttcactg gtcgtgagat agttcggcgc gatcgacggc gcctcgtcag
    14221 gcttggcgga acgtatgcgc acggtgccgc gcgaggtcgg ctgcagattg caggcgctca
    14281 ccgtgatcgc ggggaagcgg tgcagcggtt cgccaaattt gtccagcgac aatggctgca
    14341 cgtggaactg gatattggcg cggtcgcgac gcggatcgga acgggtgaaa atgccgagct
    14401 gcgacggcgc catggtcagc ggaccacggc ggcggaaggc ataatcgagg cccatcaggc
    14461 cgcgccttac aagcgaataa taagtctcgt tcagcgtgcg gacgccatcg acctggtaga
    14521 tcgcgcgctg ctgcagatga tcctgcagat tgcgcccgac gccttggcgg tcgagcacgg
    14581 tttcgatccc aagcggcgcc agccactccg caggcccgat gccggagcga tgcagcacct
    14641 gggtcgatcc gatcgagccg gcgcacagga tcacttcggc tttggcgcgg gcctcgacgg
    14701 tttcgccgcc ctggatgaac cgcaccccga ccgcgcggcc gtgctcgacg atcagccgat
    14761 cgaccagcac gtccttttca agtcgcagat tgcgacgctg caatgcgggc ttcagaaaac
    14821 ctcgcgcgga agacaagcgg cgtccgcgct tctggttgac atggaaatag ccgacgcctt
    14881 cattgtcgcc ggtgttgaag tccgggattt tgcggattcc catttcttcc gcggcgtcgc
    14941 cgaccgcgtc gagaatggcc caggacaatc ttggagcctc gatccgccag ccgccgcctg
    15001 cgccatgatg ctcgctttcg ccgagaaaat gatcctccag ccgccggaac gccggcagca
    15061 catcgtcata accccagccc gaaaggccga gctgacgcca gtggtcgtag tccgcagctt
    15121 gtcctcgcat cgagatcatg gcgttgatgg cggacgagcc gccgatcacc ttgccgcggg
    15181 gataggccag cgagcggcca ttgaggccgg cttccggctc ggttcgaaac atccagtccg
    15241 aacgcggatt gccgatcgca aagagatagc cgaccgggat gtgaaaccag atccagttgt
    15301 cgctgccgcc ggcttccagg atcaggacgc ggtttttggg atcggccgac agccggttgg
    15361 caacgatgca gccggcggtg cccgcgccaa cgacgatata gtcgaaatca ccttcaagct
    15421 gcttcataag tcatcaccgc atgcccgctt ttgggcatta gccatagcag cccatagcgt
    15481 cgctgacgcc cgctgtacag ccgccagagg agtgctagag aaggcaaagc tcctcaaacc
    15541 gaaccgaggg ttccgatgcc catcgttaat cgcgtcgccg atctgcaacc tgatatccag
    15601 gcctggcgcc aggatatcca tgcccatccc gagctgttgt acgacgtgca tcgcactgcg
    15661 gcattcgtgg cggaccggtt gcgggaattc ggttgcgacg aagtcgccac gggtctcggc
    15721 cgcactgggg tcgtcggcgt catcaagggc cgcaagcctg ccggcaagag cgatatcagg
    15781 gtgatcgggc tgcgcgccga catggatgcg ctgccgatcg aggaggagac ccatctcgcc
    15841 tacgcgtccc ggacgccggg caagatgcac gcctgcggcc atgatgggca caccgcgatg
    15901 ttgctcggcg ccgcccgtta tctcgcggaa acccggaact ttgccggcga tgccgtcgtg
    15961 atcttccagc cagcggaaga gggcggcgcc ggtgccgccg ccatgatcga ggacgggttg
    16021 atggaccgtt tcggcattga tcaggtctat ggcatgcata acggtccggg aattccgatc
    16081 ggctcgttcg cgatccggtc cggtccgatc atggcgtcga cggattcgat cgatatccgg
    16141 atcgaggggc tcggcggcca cgcggcgcgt ccgcacaaat gcatcgactc cgttttggtc
    16201 ggcgcgcagc tgatcacggc cttgcagtcg atcgtttcgc gcagcgtcaa tccgctcgac
    16261 tccgcggtca tttcgatgtg cgagtttcat gccggcgttg cgcgcaacgt gatcccgcaa
    16321 accgccatcc tcagaggcac cgtccgtacc ctgacggtgg aggtgaggga actggtcgag
    16381 aagcgggtgc gcgaggtcgt cgcaggcgtg gcgcagatca ccggcgcgaa aatcgatttc
    16441 gtctacgagc gcggttatcc cgtggtggtc aatcacgccg cgcagaccga tgtcgccact
    16501 caggtggcga aggaaatcgc cggcgacgcc aacgttcacg agacgcaccc gttcatgggc
    16561 gcggaggatt tcgcctatat gctggagtcc cggccgggcg cgtttatttt ctgcggcaac
    16621 ggcgatagcg ccggcctgca ccatcccgcc tacaatttcg acgacaacgc gatcgtgttc
    16681 ggcacctcgt actggatcag gctggtcgag accgcgctgg cggcgtgacg ttcgtacgca
    16741 gcgtgtcccg gacgcggtgc agcgcctctt cggcgatgcg ctgcggagcg gggacccatg
    16801 agaagcgcgg ttaggtaata tggaccccgg attgcggctc accacgccgc aaggcatagc
    16861 gcgtcgaaga cgcgcgtgaa cgcgctgatg gcgctgcgct gcatccgggg cacaccacta
    16921 gcatgcgatg atttcaggat gaatcgtcat cgcgctctag cctattgttt gagcatgatc
    16981 ttttcggaaa accggtaccc acttttccgg atcatgctct agctagcgtc tgccgctggt
    17041 ggcttcaaca tctcagaaca gccgcgaaaa gatcacgtag agcgagcccg acagcacgat
    17101 cgcgaccggc agcgtcagca cccacgccat caccatgttg cggatggtgg ccacctgcag
    17161 cccggagccg ttggcggcca tcgcgccggc gacgccggac gacagcacat gcgtggtcga
    17221 gaccggcagg ccgaacatgt cggcagcccc gatggtggcg gcggcgacca gctcggcgga
    17281 ggcgccttgc gcgtaagtga ggtggctctt gccgatcttc tccccgacgg tgatgacgat
    17341 gcgcttccag ccgatcatgg tgccaaggcc gagcgcgatc gcgaccgcga tcttgaccca
    17401 cctcggaatg aacttggtgg cggcatcgag cgagcttttg taggcgttca ggatgccgat
    17461 ttcttctttg ttcagatcgt tttgcttgtc tttcatcaga aaccggatcg cctccgacgt
    17521 caggtacacg tcgttgcggg tgttgccgac cgcctcggac ggaaccttgc tgagcgagcc
    17581 gtatttctgc acctggtcgc cgacatcctt caccagaacc gcaagcgagg gataagtgcc
    17641 ctcgctgagc gtgtgctggg cgacatattg ggtcaccgcc gggcgtggat cgccgataat
    17701 gctgtgaccg gcgcccttgg cggcgatcac ggtggaggcc gcggccgagg tctgctggaa
    17761 cagcgccacc tgcgattccg gcagcgcgcg gttcaatgcg taagccgtcg gcacggtgcc
    17821 gatcaggatc agcatgatca ggcccatgcc cttctggccg tcgttcgagc catgcgcgaa
    17881 gctgacgccg gtgcaggtca ggatcaacag gccccggatc cagagcggtg gcgccttgtt
    17941 accttccggg gccgcataca gcgccggatt gcggacgatc accttgagca aaagcagcag
    18001 cagcgcggcg aagataaagc cgaccagcgg cgacagcagc agcgcgtagc cgatttcggt
    18061 ggccttgctc cagtccaccc cggatgtgcc gtcgcggccg cgcatcagcg cattggcaat
    18121 gccgacgccg atgatggaac cgatcagggt gtgggaactg gaggccggca ggccgaaata
    18181 ccaggttccg agattccaga tgatcgccgc gatcaacagc gcgaacacca tcgcaaaacc
    18241 ggcgctgctt ccgacctgca ggatcagttc gaccggcaac agcgacacga tgccgaaggc
    18301 gaccgcgccg ctggagagca gcacgccgag gaagttgaag aaccccgacc acatcaccgc
    18361 gaattccgcc ggcagcgaat gggtgtagat cacggtcgcg accgcgttgg cggtatcgtg
    18421 gaagccgttg acgaattcaa agccgagcgc gatcaagagc gcgaccagca gcagcagata
    18481 gggcaggtag gtcgtcacct tgacgccggt cgcgtcgacg tcggcgtaga tgctgtaggc
    18541 gacgaacaat agcccggctg cgagaatgcc gaagaacatg atcaaggtca ggggattgaa
    18601 gcccttgtcg agattgggcc tggcagccga ctggaccggc gccccttcgt ccgtcgtctg
    18661 attgagtgca aaatcggtca tgctaacctc ctcgacgggg tgagccccaa tctttgaccg
    18721 aggttcttaa agcctggatg acagaggcgg agttattcga agtcggctgg ccgaccgatc
    18781 gagttcacga cggcaagctc actcggcccg agcctctgcg gcttgctttt gaaatgactg
    18841 gaacattgct catgaggtga cgttggagag gcggggacat ctcgtatctc tcacttacgt
    18901 ctaatcattg gagtactgcc atgaccgcga aagacaaaca cctcaacgac ctcttcctcg
    18961 ataccctgaa ggacatttat tatgccgaaa agcagatttt gagagcgctg ccgaaaatgg
    19021 cgaaggcggc cagttccgac aagctgcggg cggcgttcga gaagcatcac ggcgagaccg
    19081 aaggacaggt ggagcgtctc gaacagatct tcgaattgct cgacaagccg gcgcgcggca
    19141 agacctgcga cgcgatccag ggcatcctcg atgagggcaa ggaaatcatg gacgaataca
    19201 agggcagcga ggcactcgat gccggcatgc ttgccgccgc gcaagccgtc gagcattacg
    19261 aaatctcccg ctatggcacc ctgaagcaat gggccacgca gctcggcatg aaggacgcgg
    19321 tgcggctgtt ggatcagacc ctgcaggagg agaagaagac cgacgagagc ctgacgacac
    19381 tcgcggaagc cgcggtcaac ctcgccgctg cggcttgatt tttcttccct tctccccttg
    19441 tgggagaagg tggccatagg cggccttcgg gcgccgtcct ttaagaggac gccgatgctt
    19501 tgcatcggct atgcgaagtc cgtgccggat gaggggtcct cttcgaatcg ataggtcgcg
    19561 acccctcacc cgtcttcgct tcgcgaagcc accctctccc acaggaggag aggggaagaa
    19621 tgtgacgttc tcgctttttg agaaactaag ccgcgcgcgc cgattttcgc aacagccgcg
    19681 cgccattccg gagagtgctg tgtctcgata ccgctggagg cgcaggaggc atcgtatttg
    19741 gcggcatccg ctgggatcgc cagcttgcgc tgcacgtcca tcgcttgaat ccctttgatt
    19801 tcacttgtga aaagcgaagc catcgatccc gattgactct ctcggccatc ttagctttat
    19861 attagaacat atcatgaaca attgagccag tttctggttc tctccctgaa ccagtgacgg
    19921 cccaaccctt aggagcggcg catgcgtgag cgatcagcaa aagtggtcgc cggttttgcg
    19981 tccgatcgcg ctcccatttt ttaaaagggg cgcatgatct tgtcgccaaa ccgctcacac
    20041 tttggcggat catgcgccac cgtacgcacg aacacgcttg cgactttgcg cggcagcatc
    20101 gagcgcatcg aggcgcacgg cgatacctat aggcctgaca gggtggcgct cgggcatgcc
    20161 gacgcggatg cgacgctgca gggcggcctc gcgctgggcg cgatgcacga ggtgttcgcc
    20221 gaaggacggc acagcgccgc cgcgacgggt tttgtcacgg gcctgatggg acgggcggcg
    20281 gcacggcggc ccctg
//
LOCUS       DASWGM010000578         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2286924,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000578 DASWGM010000000
VERSION     DASWGM010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286924"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VGA15_16460"
     CDS             complement(<1..536)
                     /locus_tag="VGA15_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX9589324.1"
                     /translation="MTEPSAQFAITLAQLNPTVGDVAGNAAKARAARARAQADGADLV
                     VFPELFITGYPPEDLVLKPAFQSACRAAIEALARDTADGGPAMLIGSPWVEDGKLYNA
                     CALLDEGCIAAIRFKANLPNYGVFDEKRLFARGPAAGPVTVRGIRVGVPICEDIWLEE
                     SEDYENVVECLAETGAEIL"
     gene            complement(581..934)
                     /locus_tag="VGA15_16465"
     CDS             complement(581..934)
                     /locus_tag="VGA15_16465"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEX9589325.1"
                     /translation="MLRFWRATINTRNGLVFAIRSEQAVREELTALALALPLAWLIGA
                     TVMRRVELIAVVVLVLVVELLNTAIEKLADRLTTEHDPQIGRVKDMGSAAVGVALVMA
                     GLFWLFAIAERMGAV"
     gene            complement(936..2321)
                     /locus_tag="VGA15_16470"
     CDS             complement(936..2321)
                     /locus_tag="VGA15_16470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX9589326.1"
                     /translation="MSERWTPDSWRARPVKQVPDYPDAKALADVEAQLATFPPLVFAG
                     EARNLKRALARVAAGEAFLLQGGDCSESFAEHGANNIRDYFRVFLQMAIVLTYAGALP
                     VVKVGRVAGQFAKPRSSPIETIDGVELPSYRGDIVNDIAFTAQARTPDPRRQIEAYRQ
                     SAATLNLLRAFAKGGYASVDNVHQWMLESVKDSPQSKRYMDLADRISEALDFMRACGL
                     NFAVDSSLGTTDFYTSHEALLLGYEQAFTRVDSTSGGYYATSGHMIWIGDRTRQLDHG
                     HVEYFRGIKNPIGLKCGPSLKPDELLRLIDVLNPDNDPGRLTLINRFGSDKIGDHLPQ
                     LVRAVRREGRVVVWSCDPMHGNTISSNSGYKTRPFDRILSEVKSFFSIHAAEGSYAGG
                     VHLEMTGQDVTECIGGARAITHEDLSDRYHTVCDPRLNAEQSIDMAFLIAELLKGRGG
                     KAKPMPVAAGL"
BASE COUNT          435 a          769 c          744 g          409 t
ORIGIN      
        1 agaatttccg cgccggtctc ggcgaggcat tcgacgacgt tctcgtaatc ttccgactcc
       61 tcgagccaga tatcctcgca aatgggaaca ccgacccgga tacccctgac ggtcaccggg
      121 ccggcggccg gaccgcgcgc aaacagccgc ttttcatcga atacgccgta gttcggcagg
      181 ttggccttga aacggatggc cgcgatgcac ccctcgtcca gcaacgcgca ggcattgtag
      241 agcttgccgt cctcgaccca gggactgccg atcagcatcg ccggcccgcc gtcggcggtg
      301 tcgcgcgcca gcgcctcgat cgcggcacgg cacgccgact gaaacgccgg tttcaggacc
      361 agatcttccg gcggatagcc ggtaatgaac aattcgggaa acacgaccag gtcggcaccg
      421 tcggcctgcg cccgcgcccg cgcggcgcgc gccttggcgg cgttgcccgc gacatcgcct
      481 accgtcgggt tcaactgggc cagggtgatc gcaaactggg cgctaggttc ggtcatggcc
      541 cgatggtgcc ttgcgctggg gcaaactgca attgcatggt ttagaccgcg cccatgcgtt
      601 cggcgatggc gaacagccag aacaggccgg ccatgaccag tgctacgccc accgccgccg
      661 aacccatgtc cttgacccgc ccgatctgtg gatcatgttc ggtggtgagc cggtcggcga
      721 gcttctcgat cgcggtgttg agcaattcga ccaccagcac cagaacgacc accgcgatca
      781 gctcgacgcg gcgcatcacg gtggcgccga tcagccaggc cagcggcaac gccagcgcca
      841 gggcggtcag ctcctcgcga acggcctgct cggagcggat tgcaaagacg aggccgttgc
      901 gcgtattgat cgtggcccgc cagaaccgca gcaactcaga gtcccgcggc gaccggcatc
      961 ggcttggcct tgccgccgcg tcccttgagc aattccgcga tcaggaaggc catgtcgatc
     1021 gactgctcgg cgttgaggcg ggggtcgcag accgtgtgat agcgatcgct gaggtcctca
     1081 tgggtgatgg cgcgcgcgcc gccgatgcac tcggtgacgt cctgtccggt catctccaga
     1141 tgcacgccgc cggcatagct gccctccgcc gcgtggatgg agaagaacga cttcacctcc
     1201 gacaagatcc ggtcgaacgg acgggtcttg tagccgctgt tcgaggagat ggtgttgccg
     1261 tgcatgggat cgcacgacca caccaccacc cggccctcgc gccgcacggc gcgaacgagt
     1321 tgcggcagat gatcgccgat cttgtccgaa ccgaagcgat tgatcagcgt caggcgtccc
     1381 ggatcattgt ccggattgag cacgtcgatc agccgcaaca gttcgtccgg cttgagcgag
     1441 ggaccgcatt tcaacccgat cggattcttg atgccgcgga aatattcgac atggccgtga
     1501 tcgagctggc gggtgcggtc gccgatccag atcatatggc ccgaggtcgc atagtagccg
     1561 ccgctggttg aatcgacccg ggtgaaggcc tgctcgtagc cgagcaacag cgcctcgtgg
     1621 ctggtgtaga aatcggtggt gccgaggctt gaatcgaccg cgaagttcaa gccgcaggcg
     1681 cgcatgaaat ccagcgcctc cgaaatccgg tcggccaggt ccatgtagcg cttcgactgc
     1741 ggcgaatcct ttaccgattc gagcatccac tgatgaacat tgtcgaccga ggcataaccg
     1801 cccttggcga aggcgcgcag caggttcagc gtcgccgccg actgccgata ggcttcgatc
     1861 tgccgccgcg gatccggcgt gcgcgcctgc gccgtgaagg cgatgtcgtt gacgatgtcg
     1921 ccgcgatagc tcggcagttc gacgccatcg atggtttcga tcggcgacga gcgcggtttg
     1981 gcgaattgac cggcgacgcg gccgaccttc accaccggca gcgcaccggc ataggtcagc
     2041 acgatcgcca tctgcaggaa gacgcggaag taatccctga tattgttggc gccgtgctcg
     2101 gcaaaacttt cggagcaatc gccgccttga agcaggaagg cctcgcccgc ggctacccgc
     2161 gcaagcgccc ttttcaggtt acgggcctca cctgcaaaaa ccagcggcgg aaacgtggca
     2221 agctgcgcct cgacatcggc caatgccttg gcatcgggat agtcgggtac ctgcttgacc
     2281 ggcctggcgc gccagctgtc gggtgtccac cgctcggaca tgacgttaac tcctgagcaa
     2341 aaaccgctac ttatcac
//
LOCUS       DASWGM010000579         9614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1733206,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000579 DASWGM010000000
VERSION     DASWGM010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733206"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VGA15_16475"
     CDS             <1..421
                     /locus_tag="VGA15_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9589327.1"
                     /translation="VQIQILLLLRELQREFGMSVIFVTHDIGVAIEICDRVAVMYAGQ
                     IVEQGSLSQIVRSPIHPYARGLLASTVHGAKRGQRLETIPGTPPSLDKAPSNCSFAPR
                     CGFAEARCVEQLPPNVHVGNRIARCILAERTEIAAAT"
     gene            complement(444..692)
                     /locus_tag="VGA15_16480"
     CDS             complement(444..692)
                     /locus_tag="VGA15_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589328.1"
                     /translation="MTQPDIWYVAYGPDKTVKTDDSATGAVRATRTFKTEVDAKLFAM
                     QILAKGRTASAGTLNPHQPKQTIAPSQIELWADPGLGG"
     gene            856..1125
                     /locus_tag="VGA15_16485"
     CDS             856..1125
                     /locus_tag="VGA15_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9589329.1"
                     /translation="MRLSNESLLVILFVGLIAGWLAGQIVRGTGFGIIGDIIVGVLGA
                     LVASLLFPKLGISIGTGLVREILYSAIGAIILLLVVRLFRTGGRL"
     gene            complement(1240..1656)
                     /locus_tag="VGA15_16490"
     CDS             complement(1240..1656)
                     /locus_tag="VGA15_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HEX9589330.1"
                     /translation="MIRRFIGIATLVAAVALPVAAQAQGVPGGIERGSRDGERAAGPV
                     GAVVGGVIGGVVGGVGGILGVDERPRFRSYVVEQRRPSYQYREDVRIGAVLPEEGVTY
                     YKLPREYGVRDYRYTVVNGRTVLVDPRTHRIVEVVE"
     gene            2059..3804
                     /locus_tag="VGA15_16495"
     CDS             2059..3804
                     /locus_tag="VGA15_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX9589331.1"
                     /translation="MAAVSGARRSELGDALRACRSAFIGVGIMSCMINLLYLTGSIFM
                     LEVYDRVLPSRSVPTLVGLAILAGGLYIAQGVLDLIRGRILIRIGTSLDETLNARVFE
                     TVVRLPLMVGGRNEGLQPLRDLDNVRSFLSSMGPGAFFDLPWLPFYLAICFAFHVMIG
                     LTALVGAIILVTLTVVTEFMSRAPAREAMGLSARRNDLAATSRRNAEVLVAMGMSGRL
                     TKRWSDANEKYLAGNQRASDVSGGLGAIAKVMRMTLQSAVLAVGAYLVIHQEATAGII
                     IAGSILSARALAPVDLAIAHWKGFVAARQSWHRLNRLLESLPARATPTLLQDPSKRLS
                     VEAVAIVPPGDQKVIVTDVTFALEAGSGLGVIGPSGSGKSSLVRALVGVWQPLRGKVR
                     LDGAALDQWSSDVLGRHIGYLPQDVELFAGSVAQNICRFDTDAGSDAIIAAAKEAGVH
                     GMIIKMRDGYDTQIGEQGTALSAGQAQRVALARALYGDPFLIVLDEPNSNLDTEGDEA
                     LTRAVRAARERGAIVVVVAHRPIGIEAVDQLLVLKDGRMQAFGPKEQVLGQVLQRVAP
                     PSPIKIVSDGGVAKS"
     gene            3849..5174
                     /locus_tag="VGA15_16500"
     CDS             3849..5174
                     /locus_tag="VGA15_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEX9589332.1"
                     /translation="MIGNITKPGNNTGVRPSIRLHLIIGLTVVVILAGGIGGWGSTAE
                     ISGALIAPGSVVVESNVKKVQHPTGGVVGEVLARDGDLVKAGDVVVRLDDTVTKASLA
                     IVTKNLDGLWARGARLEAEQRGVDRIVFPKALLDRAGDPDVKNIIASETKLFEVRTFG
                     RVGQKAQLRERVTQLNEEIGGLTAQEKAKDQEIVLVEKELTGVQQLYDKHLVQMSRLT
                     TLERDAARLNGERAQFIASRAQAKGKITETELQIIQVDKDLVSDVSKDLRETNDKIGE
                     FVERKVTAEDQLRRTDIRAPQDGMVLQSTVHTVGGVITAGDAIMMIVPQSDALSVEAK
                     VNPQDIDKLQIGQKTLLRLSAFNQRTTPELNGVVTRVSPDVTTDQRTGQSYYTIRVSM
                     PAEEIKRLGDAKLIPGMPVEAFVQTGDRTMLSYLIKPLSDQLMRAFREK"
     gene            5450..5983
                     /locus_tag="VGA15_16505"
     CDS             5450..5983
                     /locus_tag="VGA15_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9589333.1"
                     /translation="MSNYRRAFVSGGCWFFTVNLLERRQTLLVDHIATLREAVATTRQ
                     SHAFTIGAFVVLPDHLHAIWNLPPGDCDFSTRWRLIKSRFARALPKRERLSAVRVARN
                     ERGIWQRRFWEHLIRDEADYARHVEYCYINPLKHRLVTRGRDWPYSSFHRDVRAGLFP
                     EDWGGHSDTNGDFGERR"
     gene            complement(5865..6767)
                     /locus_tag="VGA15_16510"
     CDS             complement(5865..6767)
                     /locus_tag="VGA15_16510"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX9589334.1"
                     /translation="MYLMAPIALWLLAVAVRFRRSALVLAGGLIAVGLVTFAAVIAGS
                     VSPHQLGLSPQTSIQSMLGFALVGLAAMYAYSPVADWLATRWVAKPPTLGAFRALAQS
                     RLKLLVGIIIAWVFGGFLEELTFRGIVLQSMQALASAWLPPPAAAGVAICAAAAGAGV
                     IHLYQGPRAVIIIVQLSVLFGVLFVVSGYNLWAVMVCHGLYDTIAFVRFAAGKSKYSR
                     LDDDHSGDEAGQPASQTTAWLQLVARRRISRRRNPPFQVNRSTIIVVRRNHRSCRYGR
                     PNLPEIVPRVHRDETRNTANRARA"
     gene            complement(8095..>9614)
                     /locus_tag="VGA15_16515"
     CDS             complement(8095..>9614)
                     /locus_tag="VGA15_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9589335.1"
                     /translation="SFEMLRVATGTDTIFIKADQAGIKSIGVKGFQIPTDRNGQLWIH
                     FAPHDPSIFVSAADVLQGTAPPEKFAGKLVLIGTSAVGLNDIKTTPVSPAMPGVEVHA
                     QVIESALTRAVLSQPSYGIVVEFCAAILFGLLVIAFAPQFGPVTLVAVGALFATLLIG
                     ISWYFYTQQRLLIDFTYPLLSTTAIYLTLIFASFVREQAQRRQIRSAFSQYLSPALVE
                     QLAQSPEKLVLGGEERELTVMFSDVRGFTTISESYKHDPQGLTALMNRFLTPLTNAIL
                     ARKGYIDKYMGDAIMAFWNAPLDDREHQVNACSAALDMLERIDVLNQQREVEAKEGGH
                     AFVPINVGVGLNTGACTVGNMGSDVKFNYSVLGDSVNLASRLEGQSKEYGFPIIVGSK
                     TALAVKEKFAILELDFIMVKGKKEPEVIYAIAGREDVAHSGRFQRLRNLTIEMLACYR
                     SRDWDGALAAIERGRRTDDAGALELLYNLYEARIRGYRENPPPEDWNGAFALLTK"
BASE COUNT         1838 a         2993 c         3041 g         1742 t
ORIGIN      
        1 cgtgcagatc cagatcctgc tgctgttgcg cgaactgcag cgcgaattcg gcatgtcggt
       61 tattttcgtc acccacgata tcggggtcgc gatcgaaatc tgcgaccgcg tcgcggtgat
      121 gtatgccggc cagatcgtcg aacagggcag cttaagccag atcgtgcgtt cgccgatcca
      181 cccttacgcg cgcggcctgc tggcgtccac cgtgcacggc gccaaacgcg gccagcggct
      241 ggaaaccatt ccgggcacgc cgccctcgct cgacaaggcg ccgagcaact gctcgttcgc
      301 gccgcgctgc ggtttcgccg aagcccgctg cgtcgagcaa ttgccgccga acgttcacgt
      361 gggaaaccgg atcgccagat gcatcctggc tgaacgcacc gagattgctg ctgcgacgta
      421 acggacgtgc acggaaaata ccttcagccg ccgaggcccg ggtcggccca aagttcgatc
      481 tgggaaggcg caatggtctg cttgggctga tgcgggttga gcgtgcccgc gctggcggtc
      541 cggcccttcg ccagaatctg catcgcaaac agtttggcgt cgacttcggt cttgaacgta
      601 cgggtcgctc ggaccgcgcc ggttgcgctg tcgtcggttt tcacagtttt atccgggcca
      661 taggccacgt accagatatc gggctgcgtc atggctgcat aacgcggaag ggccgcgaaa
      721 gtttcacacc tgaatcgaaa acgtttttcg ccgggacctt gcgtggcagc agttttggaa
      781 tagtcacatc aggcaattat cgataggttc tgacgcagcg cggagcaacc gcgtacacca
      841 actgggggaa ttttgatgcg actgtccaat gagagccttc tcgttattct gtttgtcggc
      901 ctgatcgcag gctggctcgc cgggcaaatc gtgcgcggca ccggctttgg catcatcggc
      961 gatattatcg tcggtgtgct cggcgcgctg gtcgccagtc tgttgttccc gaagctcggc
     1021 atttccatcg gcaccggcct cgtgcgcgag atcctctatt cggcgatcgg ggccatcatc
     1081 ctgttgctgg tcgtgcggct gtttcgaacc ggcggccggc tctgacgccg cggcgtcgcg
     1141 cgccggccga acaacatttt gcgaattcgg acgttggaaa ataatgggga ccatcctgac
     1201 gcacggcccc cattcgacgt ggtccatgcg acggcagtgt tactcgacga cctcgacaat
     1261 gcggtgggtg cgcggatcga ccagaacggt gcggccgttc acgaccgtgt agcgatagtc
     1321 gcgcacgcca tattcccgcg gcagtttgta ataggtcacg ccttcctccg gcagcaccgc
     1381 gccgatccgg acatcctcgc gatactgata ggaggggcgg cgctgctcca cgacgtagct
     1441 gcgaaagcgc ggacgctcgt cgacgcccaa aatcccgccg acgccgccga ccaccccgcc
     1501 aatgacgccg ccgaccacgg cgccgaccgg accggccgct ctttcgccgt ctcgtgagcc
     1561 gcgctcaatc ccgccgggga ccccttgcgc ctgcgccgcg acaggcagcg ccaccgcggc
     1621 gacaagggtc gcaattccga taaagcggcg aatcatgaca tctccagtgc tgttggggaa
     1681 aggtgcaagg acaaccgacg gggggtgatt cggttcctgc taagtcgtcc cggcgaacgc
     1741 cgggacccat acggcgagga ctctcgtttt ggcagtggag cggaggcctt ttctcactat
     1801 tgaggccagg ggtgatgggt cccggcgttc gccgggacga ctcggtgaga taactccgcc
     1861 caccccgcag ccccggtctc gtccgttcgg tcgacgcgcc aatggtgccg acctgccgcc
     1921 gccgagggcc tccaaccggg ggaggggtga agccccagcc ggcgtttacg ttaatgttaa
     1981 taaggtgact taaatggacg atagctggaa agatcggtaa gactggcggt cccaccgctt
     2041 gtaaagagat caggatagat ggcagccgtt tccggtgccc ggcgttccga gcttggtgat
     2101 gcgctgcgcg cctgccgcag cgccttcatc ggcgtcggca tcatgagctg catgatcaac
     2161 ctgctttatc tgaccggctc gatcttcatg ctggaggtct acgaccgggt gctgccgagc
     2221 cggagcgtgc cgaccctggt tggcctcgca atcctggccg gcggattgta catcgcgcaa
     2281 ggcgtgctcg acctgatccg cggacgaatc ctgatccgga tcggaacctc gctcgatgag
     2341 accctcaacg cgcgggtgtt cgaaaccgtc gtgcgcttgc cgctgatggt gggcggacgc
     2401 aacgagggcc tgcagccgct gcgcgatctc gacaacgttc gctcgtttct aagcagcatg
     2461 gggccgggcg cgttcttcga cctgccgtgg ctgccgtttt atctcgcgat ctgtttcgcc
     2521 ttccatgtca tgatcggcct caccgcgctg gtcggcgcca tcatcctggt cacgctgacc
     2581 gtcgtcaccg aattcatgtc gcgcgcgccg gcgcgcgagg cgatggggct ctcggcgcgc
     2641 cgcaacgatc tcgccgccac cagccggcgc aacgccgaag tgctggtcgc gatgggcatg
     2701 tccggccgcc tgaccaagcg ctggagcgac gccaacgaaa aatatctcgc cggcaatcag
     2761 cgcgccagcg acgtctcggg cgggctcggc gcgatcgcaa aagtcatgcg catgacgctg
     2821 cagtcggcgg tgctcgcggt cggtgcctac ctcgtgattc atcaggaagc caccgccggc
     2881 atcatcatcg cgggctcgat cctaagcgcg cgggcgctgg cgccggtgga tctcgcgatc
     2941 gcgcactgga agggttttgt ggccgcccgg cagagctggc accgcctcaa tcgcctgctc
     3001 gaatcattgc cggcgcgggc gacgccgacg ctgctgcagg atccgtcaaa acgtctctcg
     3061 gtcgaggcgg tcgccatcgt gccgcccggc gatcagaagg tgatcgtgac cgacgtcacc
     3121 ttcgcgctgg aggccggcag cggactgggc gtgatcgggc cgagcggatc gggaaaatcc
     3181 tcgctggtgc gggcgctggt cggcgtctgg cagccgttgc gcggcaaggt gcggctcgat
     3241 ggcgcagcgc tcgatcaatg gtcgtccgac gtgctcggcc gtcacatcgg ctatctgccg
     3301 caggatgtcg aactgttcgc aggctccgtg gcccagaata tctgccgctt cgatactgac
     3361 gccggttccg acgccatcat cgccgccgcc aaggaagccg gcgtccacgg catgatcatc
     3421 aagatgcgcg acggctacga cacccagatc ggcgagcagg gcacagccct ttcggccggc
     3481 caggcgcagc gggtggcgct ggcgcgggcg ctgtatggcg atccgttcct gatcgtgctg
     3541 gacgagccga actccaatct cgataccgag ggcgatgagg cgctgacccg tgcggtgcgc
     3601 gcggcgcgcg agcgcggcgc catcgtggtg gtggtcgcgc atcggccgat cgggatcgag
     3661 gcggtcgatc agcttctggt tctgaaagac ggccgcatgc aggccttcgg ccccaaggaa
     3721 caggtgctgg ggcaggtgct gcagcgggtg gcgccgcctt ctccgatcaa gatcgtgtcc
     3781 gatggcgggg ttgccaagtc atgatcgaca gcaacaccat cgccggcatc aaggccatga
     3841 ccggcaacat gatcggcaac atcaccaagc cgggcaacaa caccggcgtg cgcccctcga
     3901 tccggctgca tttgatcatt ggcctcaccg tggtcgtcat tctcgccggc gggattggcg
     3961 gctggggctc gacggccgag atttcgggcg ccctgatcgc gccgggatcg gtcgtcgtcg
     4021 aatccaacgt gaagaaagtg cagcatccga ccggcggcgt ggtcggcgaa gtgctggcgc
     4081 gcgatggcga tctggtcaag gccggcgacg tggtggtgcg gctcgatgac accgtcacca
     4141 aggcgagcct tgccatcgtc accaagaacc tcgacgggct gtgggcgcgt ggcgcgaggc
     4201 tggaggccga gcaacgcggc gtcgacagaa tcgtatttcc caaagccttg ctcgaccgcg
     4261 ccggcgaccc cgacgtcaag aacatcatcg ccagcgagac caaattgttc gaggtccgca
     4321 ccttcgggcg ggtcgggcaa aaggcgcaat tgcgcgagcg ggtgacgcag ttgaacgagg
     4381 agatcggcgg cctgacggcg caggaaaaag caaaagacca ggaaatcgtg ttggtggaaa
     4441 aggagctgac cggggttcaa caactctatg acaagcacct ggtgcagatg tcgcgcctga
     4501 cgacattgga gcgcgacgcg gcccggctta acggcgagcg cgcgcagttc attgcctcaa
     4561 gggcgcaggc caagggcaag attaccgaga ccgaattgca gatcatccag gtcgacaagg
     4621 atctggtcag cgacgtttcg aaggatttgc gcgagaccaa cgacaagatc ggcgaattcg
     4681 ttgagcgcaa ggtcacggcc gaagaccagt tgcggcggac cgacatccgc gccccgcagg
     4741 atggcatggt gctgcagtcg acggttcata ccgtcggcgg cgtgattacc gccggcgacg
     4801 ccatcatgat gatcgtgccg cagtccgatg cgctgtcggt cgaggccaaa gtcaatccgc
     4861 aggatatcga caagctgcag atcggccaga aaacgctgct gcggctgtcc gccttcaacc
     4921 agcgcaccac gccggaactg aacggcgtcg tcacccgcgt ctcgcccgac gtcaccaccg
     4981 accagcgcac cgggcaaagc tactacacca tccgcgtctc gatgccggcc gaagagatca
     5041 agcgcctcgg cgacgccaag ctgatccccg gcatgccggt cgaagccttc gtccagaccg
     5101 gcgaccgcac catgctgtcg tatttgatca agccgctgag cgaccaattg atgcgggcgt
     5161 tcagggagaa gtgaagcgtt cggcgcgaaa caatccacca cgtcgtccca gcgaacgccg
     5221 ggacccataa tccctggcgt gagtggttgt gcgaaagccg tcgaacagcg tcactcaaaa
     5281 caagcgacac ggagtatggg tcccggcgtt cgccgggacg acgatgacgg tgggccgcgt
     5341 tacgaatcct cagcgcgctc gaaagccgcc aactcgtagg gcggattagc cggaggcgta
     5401 atccgccgcg cagctacaac accaatgcat tcgtgctatg cttcgtctca tgtcgaatta
     5461 tcgtcgcgcg ttcgtttcgg gcggatgctg gttcttcacg gtcaacctgt tggagcggcg
     5521 acaaaccttg ctggtcgacc atatcgcgac cttgcgcgag gcggtcgcca cgactcgcca
     5581 aagccatgcc ttcacgatcg gcgcattcgt cgtgctgccc gatcatctgc atgcgatctg
     5641 gaacctgccg ccaggcgatt gcgatttttc cacgcgctgg cgcctgatca agagccgctt
     5701 cgcgcgagcg ttgccgaagc gggagcggct cagcgccgtg cgcgtcgcac gcaacgaacg
     5761 cggcatctgg caacgcagat tttgggagca tctgatccgc gatgaggctg actatgcgcg
     5821 ccatgtcgaa tattgttata taaatccact caagcaccgg ctcgtcacgc gcgggcgcga
     5881 ttggccgtat tcctcgtttc atcgcgatgt acgcgcggga ctatttccgg aagattgggg
     5941 cggccatagc gacacgaacg gtgatttcgg cgaacgacga tgatcgtgct gcggttgacc
     6001 tggaacggcg gattacgcct tcggctaatc cgcctacggg ctacgagctg cagccaggcg
     6061 gtggtttgcg atgcgggctg accggcttcg tctccggaat gatcgtcgtc cagtctggaa
     6121 tacttggatt ttccggcggc aaatctgacg aatgcgatcg tgtcgtagag cccgtggcac
     6181 accatgacgg cccacagatt gtaaccgctg acgacaaaca acaccccaaa gagcaccgac
     6241 aactgcacga taatgatcac cgcgcgcggc ccctggtaga ggtggatcac cccagccccg
     6301 gctgcggcgg cgcatatcgc cacgcccgcc gccgctggcg gtgggagcca ggccgaagcg
     6361 agtgcctgca tcgattgcag gacgatgcca cggaaagtca gttcctccaa aaaccctccg
     6421 aagacccagg cgatgatgat accgaccaga agttttagtc tggattgcgc aagcgcacga
     6481 aacgcaccca atgttggcgg cttggcaacc cagcgcgttg ccagccagtc agccacagga
     6541 ctgtaggcgt acatcgctgc cagtcctacc agcgcaaagc cgagcatcga ttggatcgat
     6601 gtctggggac ttagcccgag ctgatgtggt gacacagatc cggcaatcac agcggcgaag
     6661 gtcactagac ccaccgcaat caatccccct gccaacacca gggccgagcg gcggaagcga
     6721 acggcgaccg ccaaaagcca aagtgctatc ggcgccatga gatacatctg aaatacagac
     6781 atcgagcagc tccaggactg cggtttcaga ggcgggccgc aatctaggca acagccgcat
     6841 caaactctag cgtcattccc cgatgtgcaa ttgcacatcg tggatgcgcc acttggcgca
     6901 ggcccggaat ccatactccc gatcgtggtt atggattccg ggctcgcgcg gagcccgtca
     6961 tcgggcggcg cagagcgccg acccgttggc gcgccccgga atgacggact ggatttctaa
     7021 cagccccgta cggcggacta acgaaatgtg atccgccgca tcgcgatccc ggattgcacg
     7081 gcgctccatc cggctgcgct gtgtgcgcgc acggccgaat ttgttgaacc ggatcaatct
     7141 gattccacca gtccagtccc atttgcaaaa atattttcct ttccgcgcac cccaaatcat
     7201 ctctagaacg tttcccatcc cggcccgaga gaggggcgta tcatgatcgt cacggacgtt
     7261 gggtcgggat gcggtggacg cggcagcgtt ttgcatgcga tggggttgca gggcgggtcg
     7321 aaagatctgt gagcgatcac aagcacgcgg acgagagatg ttgtcgcgga cggcaaaatc
     7381 gtgtggtcct gacgccccga cgctggcgtc aagtcttgcg gaggcgtgtc ggcccaaccg
     7441 ggcgcggaca ttccatattc gcgggacgac ggtggcaaaa gagcccggtc accggggaga
     7501 gcacgacata agccgttaaa accattgcgc agggaatgcc gggtgttccg gggtacctgt
     7561 ggtgactacc gtgtgcttac taccaatgca cacgggctgc gggtgcagcg ggcacccggc
     7621 gttccctgca ccctcttcgg gcgaaagaat tcatgcacag ctcgggcgca tcgcgtcgcg
     7681 agcgcggagt cgtatgtgcg aacgacgttg ccgtttgaaa attgaatcaa aagcaattcg
     7741 ttgaatacga acgcgccaca cactctacgt cgtccccgcg aaggcgggga cccagtattc
     7801 cgaggcgcca gtgatggaat cgcaaagctg cggcgtactg gatgctccgc tttcgcggag
     7861 tatgacggtc tccgcgagga gcgccccaca ctctgcgtcg tcccggccac cgggtcggcg
     7921 catcgcgccg cccgatgaca ggctccggcc gggacccata atcccaggcg tgtgtggttg
     7981 cggaaggtgt ctgctttcgt gcctcatcga gacgacacag cgtatgggtc ccggccggag
     8041 cctgtcatcg gccggcgcga tgcgcggacc cggtggccgg gacgacgacg tggattactt
     8101 cgtcaataac gcaaaagcac cgttccagtc ttccggaggc gggttttccc gatagccgcg
     8161 gattcgcgcc tcatacaaat tatagagcag ttcgagcgcg ccggcgtcgt cggtcctgcg
     8221 gccccgctcg atcgccgcca gcgcgccatc ccagtcgcgg ctgcgatagc acgccagcat
     8281 ctcgatggtc aggttgcgta atctctggaa ccgtccggaa tgcgcgacat cctcgcgccc
     8341 ggcgatggca tagatgacct cgggctcttt cttgcccttg accatgatga aatcgagttc
     8401 gaggatcgca aacttctcct tgaccgcgag cgcggtcttg gagccgacga tgatcggaaa
     8461 accatattcc ttggattgcc cctccagccg cgaggcgaga ttgacgctgt cgcccaacac
     8521 cgaataattg aatttgacat cggaacccat gttgccgacc gtgcaggccc cggtgttcaa
     8581 gccgaccccg acattgatcg gaacaaaggc gtgaccgccc tccttcgctt cgacctcgcg
     8641 ctgctggttg agcacatcaa tccgctccag catgtcgagc gcggcactgc aggcgttgac
     8701 ctgatgttcc cggtcgtcga gcggtgcatt ccagaacgcc atgatggcgt cacccatgta
     8761 tttgtcgata tagcccttgc gcgccaggat cgcattggtc agcggcgtca ggaaccggtt
     8821 catcagcgcg gtgaggccct gcggatcgtg cttgtaggat tccgagatgg tggtgaagcc
     8881 gcggacgtcc gagaacatga cggtcagttc tcgctcctcg ccgccgagca caagcttttc
     8941 cggcgactgc gcgagctgct cgaccaatgc gggcgacaga tactggctaa aggcggagcg
     9001 aatctgccgc cgctgcgcct gctccctgac gaagctggcg aaaatcaggg tgaggtatat
     9061 cgccgtggtc gacagcaacg gataggtaaa atcgatcagc agccgttgct gggtgtagaa
     9121 ataccaggat attccgatca agagcgttgc aaacagcgcg ccgacggcga cgagggtgac
     9181 cggcccgaat tgcggcgcga acgcgatcac cagaagtccg aacaggatgg ccgcgcaaaa
     9241 ttcgacaacg atgccgtaac tcggctgcga cagcaccgcg cgggtcagcg cgctctcgat
     9301 cacctgggcg tggacttcca cccccggcat ggccggggaa accggcgtgg tcttgatgtc
     9361 gttgaggccg accgccgagg ttccgatcag caccagcttg cccgcaaact tttccggcgg
     9421 ggcggttccc tggagtacgt ccgccgctga gacaaaaatc gagggatcat gaggcgcgaa
     9481 atgaatccag agctggccgt tgcgatcggt cggaatctgg aaacccttga cgccgatgct
     9541 cttgatgccg gcctggtcgg ccttgatgaa aatcgtgtcg gtgccggtcg ccacccgcaa
     9601 catttcgaaa ctca
//
LOCUS       DASWGM010000580         8192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2563657,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000580 DASWGM010000000
VERSION     DASWGM010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2563657"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="VGA15_16520"
     CDS             <1..152
                     /locus_tag="VGA15_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEX9589336.1"
                     /translation="TVTKTPAEAVGLTDRGEIAPGKRADLIRVHIARDIPVVRSVWRE
                     GHRVA"
     gene            149..733
                     /gene="phnN"
                     /locus_tag="VGA15_16525"
     CDS             149..733
                     /gene="phnN"
                     /locus_tag="VGA15_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HEX9589337.1"
                     /translation="MTQASAISDHRPAAIGPGRLILVVGPSGAGKDTLIGWAAAACAN
                     DGNIVFPRRVVTREASPSEDNEQVSLDAFLQARSKDEFAVQWEAHGHCYALPRAINDD
                     IRGGRTVVANVSRTVVDAVRRAYAAVTVVSITAPPDILAERLAARARSSDGPLADRLR
                     RAVDDAAAVPDVTIINIGSVEDRAREFVQVIRRP"
     gene            781..1392
                     /locus_tag="VGA15_16530"
     CDS             781..1392
                     /locus_tag="VGA15_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9589338.1"
                     /translation="MTIIATIEQLEAIYGQPNEASTVKVADRITPQYRVLIDKSPFAA
                     LATCGPEGLDCSPRGDLPGFVRVHDETTLMMPDRRGNNRVDSLRNIVRDPRIALLFLI
                     PGSGSTLRVNGRARVSADADLLASFKVDGKAPRTVIVMTVDEIYFQCARAIVRSDLWN
                     PDRRVDPKSLPTPGQILAEMSENRVGGEDYDRAWPERARQTMW"
     gene            complement(2176..4341)
                     /locus_tag="VGA15_16535"
     CDS             complement(2176..4341)
                     /locus_tag="VGA15_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589339.1"
                     /translation="MKRLKDFLGASIPLALGAVFLGAASQSASAACLLNSPGGQIKHV
                     VHIVFDNVHLRRDNPDVPSDLEQMPNLLNFIQNNGTISGNHHTPLISHTATDILTAQT
                     GVYGNRMGIPVSNSYGFFRPDGSVGFTSSFLYWTALSGDGKPQMLNELGKAAPAPWVP
                     FTRAGCDVGGFSVANIEFERVPDDVTTFYGAGSAVDTAVRAQLASASPATRQQPTTDY
                     LGVAIHCAVGSPRCNNGFATSDPLPDEPNGYNGFKGLFGNINVQPVISPSGPVKDLDG
                     NVIADAFGRPGFPSGFSPRATQSLGYAATMLEAGVQVVYLYISDAHDNQSGSGTFGPG
                     EAGYVAQLKQYDTAFGKFFARLGADGITKDNTLFVVVPDENDHFVGTATPSPAHCDGV
                     NVPCTYAAGQKGEINAIINRLLITQRANTTAFSVHSDDAPTVYINGNPAPTATATRTM
                     EHDLDALVATNPMTGNSDKLSKLLADQAEMKLLHMVTASPARTPTLTMFGNDNYFFTT
                     GAGGACANQSACIFVGPGFAWNHGDFQRDITRTWVAMVGPGVQRLGRHDAVFSDHTDV
                     RPTLLALLGLKDSYVHDGRVLSEWMEQRALPSAIRQSRENFSELAEVYKQLNAPLGEL
                     GRASLVYANRSVTGVDTAYARYLAKISDITNQRDSLAGDIKTVLNAAAFGNQPVDERS
                     EDGLGRRARELIDLVKDLAEKDSAEHDHRDEDHDHRAEN"
     gene            complement(4625..5086)
                     /locus_tag="VGA15_16540"
     CDS             complement(4625..5086)
                     /locus_tag="VGA15_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9589340.1"
                     /translation="MLFGAGTTRAAFADGLSRATAAYSRGDYVRAANELSPLALRGNA
                     RAQTLLGFMYENGFGVPQAYDAATDLYQRAAVQGDPSAQSRLGLSYDKGHGVPQSVVL
                     AYKWLDLAAAHAPKRERDYYLRLRNAVASKMSSDQIVEGQRLALFWAPGRW"
     gene            complement(5190..7448)
                     /gene="gspD"
                     /locus_tag="VGA15_16545"
     CDS             complement(5190..7448)
                     /gene="gspD"
                     /locus_tag="VGA15_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX9589341.1"
                     /translation="MLLSAGLLASCNPVTVSSGSGADIDVLDKVRSLDTLPRYPQQAN
                     AAEANAGQRSRPVMFEGTEVSDVAEARPQPVATGNGFELNFENTPIATVAKVVLGDIL
                     QTGYTIDPRVQGTVSLVSVRPVAKSDIVFVLENALRLSGIVLVRDTAGYRLTPLGDAV
                     GAGRVDAAASSPEPGYGVSVVPLQYVSAQTLLKLMDSFATKAGMVRADTTRNLLLIQG
                     SGAERRTAVDTALSFDLDWMRGQSIGIFPIVNSGPEPVIAELEKIVDSGDNGLSQNLI
                     KFQAVSRLNAILVVSKKPALLHTAATWIKRLDHADTARTSVHVYRVKYGEARQIARVL
                     TEMFIGGSPSGLLDSPDSQIAPGSGTYATASADRLSLNNNSPSSTSSNGFGSRMQSGT
                     NGNSGFGAQTAAAPGGANANTPDAGSLDSRGAGSGGQPMLKGVRITADTVNNSLLIYA
                     DQENFRIIEATLQQVDQPQLQVAIDATIAEITLTDELSYGVQSYLTSHNLGLRPDQGS
                     ILNTQSTSAPVAAAAGTVANAFINRAFPGFNFLIGSEAQPSAILDALHTVTAVKVLSN
                     PSLVVVNNQAATLQVGDSVPVSTGSATVLTTNNTVVNTIDYRNTGIILRVSPRINVNG
                     NVRLDVEQEISNVSAATAASLTPTVSERKVKSSISVATGQTVLLAGLISEQHNNQRNG
                     IPLLDQIQGLGDAFSHQDNKATRTELIIFIRPQIIRNSMDAHFVAEELRSKLRGSINA
                     SVANDTRVQGVR"
     gene            7866..>8192
                     /locus_tag="VGA15_16550"
     CDS             7866..>8192
                     /locus_tag="VGA15_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEX9589342.1"
                     /translation="MTGDESLQFVEHLRHKHHLMETAGNGAGAEQGGSARSPGKLWEL
                     TDLSASDFADEVARFFDLDRVTLQDMLSAPSLVAAFSQRFLREMMLFPYQSADGGAVL
                     AVADPTD"
BASE COUNT         1519 a         2388 c         2656 g         1629 t
ORIGIN      
        1 gtacggtcac caagacgccg gccgaagcgg ttggcttgac cgatcgcggc gagatcgctc
       61 cgggcaagcg cgccgatctg attcgcgtgc atattgcccg cgacattccg gtggtgcgca
      121 gcgtctggcg tgaaggacat cgcgtggcgt gacgcaagcc tcagccatat cggatcatcg
      181 gccggctgcg atcgggcctg gacggctcat tctggtggtc ggtccgagcg gcgccgggaa
      241 ggacacgctg atcggatggg ccgcagccgc ctgtgccaac gacggcaata tcgtctttcc
      301 acgtcgcgtg gtgacgcggg aagcctcgcc ttccgaagac aatgagcagg tgagcctgga
      361 tgccttcctg caggcgcgca gcaaggacga gttcgccgtt caatgggagg cgcatggcca
      421 ttgctacgca ctgccccgcg cgatcaatga cgatatccgt ggcgggcgca cggtggtcgc
      481 caatgtgtcg cgcacggtgg tcgacgcagt gcgtcgcgcc tacgccgcgg tcacggtggt
      541 gtcgatcacc gcgccgcctg acattctcgc cgaacgcctc gccgcgcggg cgcggagcag
      601 cgacgggccg cttgcggatc ggctccggcg cgccgtcgat gatgccgcgg ctgtgcccga
      661 cgtcaccatt atcaacatag gcagcgtcga agatcgcgcg cgcgagttcg tgcaggtcat
      721 caggagacct tgacgtaagc gggcgctgcg cggccagccg cagagagcag gagaacgaca
      781 atgacgatca ttgcgacgat cgaacagctt gaagcgatct acggacagcc gaacgaggcc
      841 tctaccgtca aggtcgctga ccgaataacg ccgcaatatc gcgtgctgat cgacaagtca
      901 ccgtttgccg cgctggcgac ctgcggaccg gagggactgg attgctcgcc tcgcggcgat
      961 ttgcccggct tcgtccgcgt gcacgacgaa acgacgctga tgatgcccga tcgccgcggc
     1021 aacaatcgcg tggattcgtt acgcaatatc gtacgcgatc cccggatcgc gctgctgttt
     1081 ctgattccgg gttctggcag cacgttgcgc gtcaatgggc gtgcgcgggt gtcagccgat
     1141 gcggacctgc tggcttcgtt caaagtcgac ggcaaggcgc cgcgcacggt catcgtcatg
     1201 acggttgatg agatctattt tcagtgcgcc cgcgcgatcg tccggtccga tctctggaat
     1261 cccgacaggc gggtcgatcc caaaagcttg ccgacgcccg gtcagatcct cgccgagatg
     1321 agcgaaaatc gtgtcggcgg tgaggactac gaccgggcct ggccggagcg ggcgcggcaa
     1381 acgatgtggt agcggaccgc tgggatcgcg acggatcgcc cggcgagtga actcgctgcc
     1441 gatcatttcc ggtggcgatc ctttcggata aactcccggg taacgggaaa attcgtgagg
     1501 gtacagccgc gttaagcgtt gccgggcgtg tctggctgaa tacgaaatat ccatcactcg
     1561 caaaagtgtc ggcaacattg tgaggtcgct cagaaagaga ctgataactg aggctcgtcc
     1621 ttgctagcgc cgcattgact taggacaatg cgtgcggtcg tcaaaacgcg cttagactga
     1681 ttccatgaaa atatccgact ttgcttgtcc gtcctgcgcc gcgtcctacg aggttgctga
     1741 atcaacgtcg gcggaaggaa gcccaggccg ggccgaatgc accatatgcg gccggcttct
     1801 ggaatcgtgg caggaaccca agatgaaagc ctatcgcttg gttttgtcgc cggaacataa
     1861 atatccgcac attcccgcgc cgccgtctcc aattgtctcc tgatttcaac gccgggcacg
     1921 ctctattccg agctaacccg ataggctcgg gatccgatct cgacaatccg gcgcccggat
     1981 tggcgcgcgt gccgggcggg tgcgggccat gaaatgtttg gtcggcctga taatcttcgt
     2041 gcagccgtcg ctttgaagtc cgtgacgcgg attgacggaa tgtcgcaaca atttcggtcg
     2101 ggctgcaaag gtctacgtgc gaaaaaaccc gccggcttgc accggcgggt tttgggttgt
     2161 tcaatttccg aaaactcaat tctcggcgcg atggtcgtga tcctcgtcac gatggtcgtg
     2221 ttcggccgag tccttttcgg ccaagtcctt gaccaggtcg atcagttccc tcgcgcgacg
     2281 accgaggcca tcttcggaac gctcgtcgac cggctgattg ccgaacgccg cggcattcag
     2341 caccgtcttg atatcgccgg ccagactgtc gcgctggttg gtgatatcgc tgattttggc
     2401 aaggtagcga gcgtaagccg tgtcaacgcc cgtgacggaa cgattggcat agaccaggct
     2461 cgcccggccg agttcgccca ggggcgcatt gagctgtttg tagacctcgg caagctcgct
     2521 aaagttttcg cggctctggc gaatggcgga aggcagcgcc cgctgctcca tccattcgga
     2581 cagcacccgc ccgtcgtgta catagctgtc tttcaggcca agcagcgcaa gcaacgtcgg
     2641 ccgcacgtcg gtatgatccg agaacaccgc atcgtggcgg ccgagccgtt gcacgccggg
     2701 acctaccatt gcgacccagg tccgggtgat gtcgcgctgg aagtcgccgt ggttccaggc
     2761 aaagcccgga cccacgaaga tgcaggcgga ctggtttgcg caggcgccgc cggcgccggt
     2821 ggtgaagaaa tagttgtcgt tgccgaacat ggtgagcgtc ggggtgcgcg ccgggcttgc
     2881 ggtcaccatg tgcagcagtt tcatttccgc ctgatcggca agcaatttgg acagtttatc
     2941 ggaattgccg gtcatcggat tggtggcgac cagcgcatcg agatcatgct ccatggtgcg
     3001 ggtcgcggtg gcggttggag cggggttgcc gttgatgtag accgtcggcg cgtcgtcgct
     3061 gtgcaccgag aacgccgtgg tgttggcgcg ctgtgtgatc agcaggcggt tgattatcgc
     3121 gttgatctcg cctttctggc ccgccgcgta ggtgcagggc acgttaacgc cgtcgcaatg
     3181 cgccgggctc ggagtggcgg tgccgacgaa atgatcgttc tcgtcgggta caacgacaaa
     3241 cagggtgttg tctttggtga tgccgtccgc gccgagccgg gcaaagaatt tgccgaacgc
     3301 ggtgtcgtat tgcttgagct gggcgacgta gccggcttcg ccggggccga atgttcctga
     3361 accggactgg ttgtcgtgcg cgtcggagat atagagatag accacctgga cgccggcctc
     3421 gagcatcgtc gcggcgtagc cgagcgattg cgtcgcccgc gggctgaagc cgctgggaaa
     3481 tcccggccgg ccgaacgcgt cggcgatgac gttgccgtcg aggtccttca ccgggccgct
     3541 cggcgagatc accggctgaa cgttgatgtt tccgaacaac cccttgaagc cattatatcc
     3601 attgggttcg tccggcaacg gatcgctggt cgcaaagccg ttattgcagc gcgggctgcc
     3661 cacggcgcag tggatcgcga cgccgaggta gtcggtggtc ggctgttgcc gcgtcgccgg
     3721 gctagctgat gcaagttgag ccctcaccgc ggtgtcaacg gcgctgccgg cgccataaaa
     3781 cgtcgtgacg tcgtcaggga cccgctcgaa ctcgatattg gcgacggaga atcctccgac
     3841 gtcgcagccg gcgcgggtga acggcaccca cggagccggc gcggccttgc cgagttcatt
     3901 gagcatctgc ggcttgccgt cgccgctcag cgccgtccag tacaggaacg aactggtgaa
     3961 gccgacgctg ccatccggcc tgaagaagcc atagctattg gaaaccggaa ttcccatgcg
     4021 gttaccataa acgccggtct gggccgtgag aatatcggtg gcggtgtgcg aaatcagcgg
     4081 agtgtgatga ttgccgctga tggtgccgtt gttctggatg aaattgagca ggttcggcat
     4141 ctgttcgaga tcggacggaa cgtccggatt gtcgcgccgc agatgcacgt tgtcgaaaac
     4201 gatatgaaca acgtgtttga tctgaccgcc tggagaattc agcagacaag ccgccgaggc
     4261 ggattgcgaa gcggcaccga gaaatacagc gccaagcgcc aatggaatcg acgcgccaag
     4321 aaaatctttc aatcgtttca tcgctctctc ctgaggtcgg cccaagtgga gtaaatctgt
     4381 gaaaaaaccg cgcagtctag ttgccgacgg cggcacaata cattgcagat tgttgcgggc
     4441 ggttgacgcg gacatgacgc tcgtgcgtgg cgtgatgtca tgcaatcgga ggagtggctc
     4501 tcaccgaaga gatttcggtt gatgaggaaa gtccgaccga acacatcatg gagtacgatc
     4561 gcgaaccaat ccgacgcgat cgcatccagg cgtaattttc gtcatgtcgg aagcgcagcg
     4621 gcggtcacca gcgtcctggt gcccagaaca aagcaagccg ctggccttca acgatctgat
     4681 cggacgacat tttcgaagcc accgcgttgc gaagcctgag ataatagtcg cgctcgcgct
     4741 tcggggcatg tgccgcggcc aggtcgagcc atttatacgc gagcacgacg ctttgcggca
     4801 cgccgtggcc cttgtcatag ctcaaaccta atctgctctg tgcagaggga tcgccctgca
     4861 ctgctgctct ctggtagaga tcggtggcgg cgtcgtaggc ctgcggcaca ccgaagccgt
     4921 tttcgtacat gaagcccagc agcgtctgcg cccgcgcgtt tccgcgcagc gccagcgggg
     4981 ataattcgtt ggcggcccgc acgtaatcgc ccctggaata ggctgcggtc gcgcggctca
     5041 gtccgtcggc gaaggcggcc cgcgtcgttc cagcgccgaa cagcaagatg acgaacagaa
     5101 gcgtcggtat ggtccgtcgc actgcggccg ccttcaggag cgttgaacaa agtcccgccg
     5161 aggcagagcc cgcgatttgc gtcctggcgt caacgaacgc cctgtactcg tgtgtcattt
     5221 gctacgcttg cgttaatgct gccacggagt ttcgaccgaa gttcttcggc gacaaaatgg
     5281 gcatccatgc tgttgcggat aatctgggga cggataaaga tgatcaattc ggtacgagtc
     5341 gccttattgt cctgatgaga gaaggcatcg cccagacctt gaatttggtc gagcagggga
     5401 attccgttgc gctggttgtt atgttgctcg ctgatcaatc cggccaacag caccgtctgg
     5461 ccggtggcca cggaaatcga actcttgacc tttcgttcgg agactgtcgg agtcaaactc
     5521 gcggcggtag ccgccgaaac gttgctgatt tcctgctcga cgtcgagcct gacatttccg
     5581 ttgacgttga tgcggggaga aacccgcaga atgatgcccg tgttgcggta gtcgatggtg
     5641 ttgacgaccg tgttattcgt cgtcagcacc gtagcgctgc cggtggagac tggcaccgag
     5701 tcgccgacct gaagtgttgc cgcctgattg ttgacgacaa ccagcgacgg gttggacaac
     5761 accttgacgg cggtgacggt atgcagggcg tccaggattg cgctcggctg cgcctccgat
     5821 ccgatcagga aattgaatcc cgggaatgcg cgattgatga aggcgttggc gaccgttccg
     5881 gcggccgcgg cgaccggcgc cgacgtcgac tgggtgttga ggatggagcc ctggtcgggc
     5941 cttagtccca gattgtggct ggtcaggtag ctctggacgc cgtagctcaa ctcgtcggtg
     6001 agcgtgatct cggcgatggt cgcatcgatc gcgacctgca gctgcggttg atccacttgc
     6061 tgcaacgtcg cctcgatgat ccggaaattc tcctgatcgg catagatgag cagggagttg
     6121 ttgacggtat cggccgtgat ccgtacgccc ttcagcatcg gctggccgcc ggagccggcg
     6181 cctcggctgt cgagcgatcc ggcgtcaggc gtatttgcgt ttgcgcctcc cggcgcggcg
     6241 gcggtttgcg cgccgaaacc ggagttgccg ttggttccgg attgcatgcg ggagccgaag
     6301 ccgttcgacg acgttgacga cggagagttg ttgtttaagg aaaggcgatc ggcgctggcg
     6361 gtcgcatatg tgcccgaccc cggcgcgatc tggctgtcgg ggctgtcgag caggcctgag
     6421 ggcgatccgc cgatgaacat ctcggtcagc acgcgcgcga tctggcgcgc ctcgccatat
     6481 ttcacccggt aaacgtgaac gctggttcgc gcggtgtcgg catgatcgag ccgtttgatc
     6541 caggtcgcgg cggtatgcag cagtgccggc ttcttgctga ccaccaggat ggcgttgagg
     6601 cgactaaccg cttgaaattt tatgaggttt tggctgagac cgttgtcgcc ggaatccacg
     6661 atcttttcga gttccgcgat cacgggttcc ggaccgctgt tgacgatcgg aaaaatcccg
     6721 atcgattgac cgcgcatcca gtcgaggtcg aagctgagcg cggtatccac cgctgtgcga
     6781 cgttccgcgc cgctgccttg aatgagcagc aggtttcgcg tcgtgtcggc gcggaccatt
     6841 ccggccttgg tcgcgaaact gtccatcagc ttcagcagcg tttgcgctga cacgtactgc
     6901 aagggcacga cggagacgcc gtagccgggt tcggggctgg aggccgccgc atcgacccgg
     6961 cctgcgccga ccgcgtcgcc gagcggcgtc aaacggtagc cggcggtgtc gcgaaccagc
     7021 acgatcccac tgagccgcaa ggcattttcg agcacgaaga caatgtccga cttggccacc
     7081 ggcctgacgg atacgaggct gacagtgccc tgcacgcgcg gatcgatcgt gtagccggtt
     7141 tgcagaatgt cgcccaacac cactttggcc acggtggcga tcggcgtgtt ttcgaaattg
     7201 agctcgaagc cgttgccggt ggcgaccggt tgcgggcgcg cctcggcgac gtcggacacc
     7261 tcggtaccct cgaacatcac cggccggctg cgctggccgg cattggcctc ggcggcgttt
     7321 gcctgctgtg gataacgggg caaggtatcc agcgatcgga ccttgtcgag cacgtcgata
     7381 tcggcgccgc tgccggagct cacagtcacc ggattgcagg atgccagcag gccggcggac
     7441 aacagcataa agccaataca aatgctgcga ccgactggcg gagaccagcc ctggttggcg
     7501 acgcgtacca tatttccgcc gttccagcgt tatgttcggc ccaaagatcg ctcgggagga
     7561 ttgatccaag ctggctggca tacttagcgg cggaacatga cagttataat tgccggcccg
     7621 gtctatcggg cagatcgacg ccacctgtgg ccaacatcca acatattgcc gttcgcgcct
     7681 tgccgcgcgg ccgggtccaa tgaacaggca aacgccaaat tcatgtgaag cggtgcacgc
     7741 ttcgtacagg tgtcatgttt cggatcaata accacactgc ggccggcaac tggccgacgg
     7801 cgggccgaag cgccgtgctc cactgatcgg cctgaagaaa aaagctgtgt gagtggaaag
     7861 acataatgac cggcgacgag tccttgcagt tcgtggaaca tctccgtcat aagcatcatc
     7921 tgatggagac cgccggcaac ggcgcgggcg cggagcaggg cgggtctgcg cgttcgcccg
     7981 gcaaactttg ggaactcacc gatctttcgg ccagcgattt cgcggatgag gtggcccgct
     8041 tttttgatct tgatcgggtc acgctccagg acatgctgtc cgcgccatct ctggtcgcgg
     8101 cattttcgca gcggtttctg cgggaaatga tgctgtttcc atatcaatcc gccgacggag
     8161 gcgccgtgct ggcggtcgcc gatccgaccg at
//
LOCUS       DASWGM010000581         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1857598,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000581 DASWGM010000000
VERSION     DASWGM010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857598"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VGA15_16555"
     CDS             complement(<1..1205)
                     /locus_tag="VGA15_16555"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX9589343.1"
                     /translation="MEVLTTAEMERADQLTIAAGTPGFALMLSAGQAVAEAAMELVEE
                     GPVVVVAGRGNNGGDGFVAAAELAARGREVSVILLCERDSLQGDAASAARGWKYPVLP
                     FNPQAIGRPALIIDALFGAGLNRPVKGEPHDMIEAINANGAPVLSVDLPSGINGDTGA
                     VMGVAVRATETVTFFRRKPGHLLLPGRIYCGRVRLADIGIDPRVLEEIRPRTFENIPQ
                     FWRNAFPVPRIDGHKYARGHAVVVSGDLAATGAARMSARGALRAGAGLVTVASPRDAL
                     AVNATALTAVMVRPIDTPVEFAELWTDKRLNTCVIGPGAGVGDRTRNFVVTALSAKRN
                     LVLDADALTSFADAPDHLFEAIKASHDPQVVLTPHEGEFPRLFSDISNKHPGRSKLER
                     VRAAAERSGA"
     gene            1587..1925
                     /locus_tag="VGA15_16560"
     CDS             1587..1925
                     /locus_tag="VGA15_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX9589344.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHAE
                     LYRGAEYIVDFLPKVKIEIVISDDLVERAIDAIRRAAQTGRIGDGKIFVSNIEEAVRI
                     RTGESGLDAI"
BASE COUNT          443 a          673 c          612 g          376 t
ORIGIN      
        1 gcgccggagc gttcggcggc cgcccgcacc cgctcgagtt tcgagcgacc cggatgcttg
       61 ttgctgatat cgctgaacag ccgcggaaat tctccttcat ggggcgtaag aacaacctgc
      121 ggatcgtgcg aagccttgat cgcctcgaac agatgatcgg gcgcgtccgc aaagctcgtc
      181 agcgcgtccg catccagcac caggtttcgc ttcgcggaca acgcggtcac gacaaaattc
      241 cgcgtgcggt cgccaacgcc ggcgcccggt ccgatcacgc aggtgttgag ccgcttgtcg
      301 gtccacagct cggcaaactc gaccggcgta tcgatggggc gaaccatcac ggccgtcagc
      361 gcggtggcgt tgaccgcaag cgcatccctc ggtgaggcta ccgtcaccag tccggcacca
      421 gctcgtagcg cgccgcgcgc ggacatccgc gccgcgccgg tcgcagcgag gtcaccggag
      481 acgaccacgg catggccgcg ggcgtattta tggccgtcga tccgcggcac cggaaacgcg
      541 ttgcgccaga actgcggaat gttttcgaaa gtccgtggac gaatttcctc gagcacgcgc
      601 ggatcgatcc cgatatcagc aaggcgcacg cgaccgcaat agatccgccc cggcaacaat
      661 aaatgccccg gcttgcgccg gaagaacgtg acggtctcgg tggcgcggac cgcgacgccc
      721 atgacggcgc cggtgtcgcc attgatgccg ctcggcaggt caacgctcag aaccggcgca
      781 ccgttggcgt tgatcgcctc gatcatgtcg tgcggttctc ctttcacggg ccggttgaga
      841 ccggcgccga acagcgcatc gatgatcagc gccggcctgc cgatcgcttg tggattgaac
      901 ggcagcaccg gatatttcca tccgcgtgcc gcggaggccg cgtcgccctg caggctgtcc
      961 cgctcacaca agaggatgac ggagacctcc cggccgcgcg ccgccagttc agcagcggcc
     1021 acaaaaccgt ctccgccatt gttgccgcgc ccggcgacca ccacgaccgg cccctcctcc
     1081 acgagctcca tcgcggcctc ggcgacggcc tggcccgcgc tcagcatcag cgcgaaaccg
     1141 ggagtacccg cggcaatggt aagctgatcg gcacgctcca tttcagctgt ggtaagaact
     1201 tccattgcca aatccctggt acaagcgcct catcctgagg cacttcctat gccgcgcgaa
     1261 ccccaacccc tgtcgtgtgc ataccgattg acctgtttgc ctatttcgca ggctacggta
     1321 ttgtaacctg ttcgctccaa ccccccgtta atgcgtgtta aaagtctgat aacatttcaa
     1381 aaatcagccg cctcaacaac ttggcataga ccctgctttt gaggaagccg gctcctgctt
     1441 cgtcgtactc acggcaatga atgaaggtac tcggctgctc gccccgaagt gagacggcac
     1501 cgcgaggggc cgaacaaccc gcgcatcacg cgcaacgtgg aaacgaacgt gcagtctaaa
     1561 atatggaagt gcctgggagg cgttcagtga aaaaaataga agccatcatc aagccattca
     1621 agctcgacga ggtgaaagaa gcgcttcagg aagtcggact gcagggcatc accgtcaccg
     1681 aagccaaggg tttcggccgg cagaagggtc acgccgaact ctatcgcggt gcggagtaca
     1741 tcgtggactt tctacccaag gtcaaaatcg agatcgtgat ctcggatgat ctggtcgaga
     1801 gggcgatcga cgcgatcagg cgtgcggccc agaccgggcg tatcggcgac ggcaagatct
     1861 tcgtgtccaa catcgaggaa gccgttcgca tcaggacagg ggaatccggg ctggatgcca
     1921 tctgaatcga ccttctccat actctgatat tgtattgagc attccccaaa tgtattgagc
     1981 attacccgac ggtcgctgcc cgcggccaga tcgacctgta gccaaaaggg gtattcatga
     2041 agaccgccaa agacgtcctt aaatcaatca aggacaacga cgtgaaatac gtcgaccttc
     2101 gttt
//
LOCUS       DASWGM010000582         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1857838,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000582 DASWGM010000000
VERSION     DASWGM010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857838"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..>1558
                     /locus_tag="VGA15_16565"
     CDS             348..>1558
                     /locus_tag="VGA15_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX9589345.1"
                     /translation="MTVTLGSRTERFQVGKALRRKTPREAHADLKGSMSRNAVAILAE
                     SDPDRVPELVPERYARMTVNPFAFLRGAAAVMATDLAAQPMAGIPVQAGGDSHLMNFG
                     AFVTPEDNILFDVNDFDETLPGVDFTIDLKRLAASVAVAASAAGTNKKQARAIAAATV
                     KAYRTHISALAKLSPLEIWHSRIDLEQAIKQIGNSDLRRQLVGVIAKARGEGLSKDDN
                     FPHLVSGDHPKIVDKPPTIFHLDPKADARHRLGVERVFAAYRKCLNPDRQRLLDRFTM
                     KDLAFKAVGVGSVGTFCCVALFMTDDNEPLFLQVKQAQRSVLERLGGKLAYKGNQGRR
                     VVEGQQMMQAASDIFLGATQDDATGRQFYVRTLKNRRLGAVSEISEGEALSDYAQLCG
                     RTLARAHARSG"
BASE COUNT          305 a          465 c          483 g          305 t
ORIGIN      
        1 gaaacttgaa cagatagcag tgaaagctgt gaaccaactg ccgaagagcg tggtgcggga
       61 aatccgcacg ccacgttctg tgggagccgg gggcgggcga ccgcctccgg cgacccggtg
      121 ggcgaggaga aatcctcgtc ctgaccgcga ccatgggctg gttgttcctt tggtcccctc
      181 ccaactcttt cggcccggat tcaccgataa gatgcgcagc tcgctgattg tcctggtggg
      241 tctctggccg tccagggctg gaccggcgta cggcacggtc accgttgccg gcttgtgaca
      301 actaggcggc ttcatatacc gtttggcttt cattgaagga cttccagatg acggtcactt
      361 tgggttcgcg taccgagcgg ttccaagtgg gcaaagcgct ccgacgcaag acgccgcgag
      421 aagcacatgc cgaccttaaa ggatcgatgt cgcgcaatgc cgttgcaatt ctcgccgaga
      481 gcgatccgga tcgcgttccg gagcttgtgc cggagcgtta tgcgcgcatg acggtcaacc
      541 cgttcgcgtt cctgcgcggc gccgccgcgg tgatggcgac agacttggcc gcccagccaa
      601 tggccggaat accggtgcag gcaggcggcg acagccacct gatgaacttc ggcgccttcg
      661 ttactcccga ggacaatatc ctgttcgacg tgaacgattt tgatgagacg ctgcctggcg
      721 tcgatttcac tatcgatctg aagcgtctcg cagcgagcgt agcggtagcg gcatcggccg
      781 caggcacaaa caagaaacag gcccgcgcga tcgcggctgc gactgtcaag gcctatcgga
      841 cgcacatttc cgcactcgcc aagctgtcgc cgctggaaat ctggcacagc cggatcgacc
      901 tcgaacaggc gatcaagcag atcggcaata gcgatctgcg gcgccagctc gttggcgtga
      961 ttgctaaggc gcggggcgag ggcttgtcga aggacgacaa ttttccgcat ctcgtcagcg
     1021 gcgatcatcc gaagatcgtc gataagcccc ctactatttt ccatctcgat cctaaggccg
     1081 acgcgcgaca ccggcttggt gtcgagcgcg tgttcgctgc ctatcgtaaa tgcctcaatc
     1141 ctgatcgtca gcggttgctc gaccgtttca caatgaagga tctcgccttt aaggcggtgg
     1201 gcgtcggatc cgtcggcaca ttctgctgcg tggcattgtt catgaccgac gacaacgagc
     1261 cgttgttctt gcaggtcaaa caagcgcagc gatcggtttt ggaacggctc ggcggcaagc
     1321 ttgcctacaa aggaaaccag ggccgccggg ttgtggaggg ccagcagatg atgcaggccg
     1381 ccagcgacat ctttctcggt gctacgcagg acgatgcgac cggccgtcag ttctatgttc
     1441 ggacactgaa aaaccgcagg cttggcgccg tcagcgaaat cagcgaaggc gaggcactct
     1501 cggactatgc ccagctatgc ggtcgaaccc tcgcgcgtgc ccatgcgcgc tccggcga
//
LOCUS       DASWGM010000583         8332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_739115,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000583 DASWGM010000000
VERSION     DASWGM010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739115"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..770
                     /locus_tag="VGA15_16570"
     CDS             267..770
                     /locus_tag="VGA15_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX9589346.1"
                     /translation="MLDGLRQLITDIVSPTEHQDRAFDDTGYRLAATALLVHVISLDG
                     EPSEIEKRKLHSLLERRFGLDPGTADQLIASAMLVEGEAVDLYHFTSVIMRSVNEEGR
                     LRIVEMMWELVYADGQVSEFEDNVVWRAADLLGVSSRDRIDLKHRVAAKLATPATDNS
                     DIADPAM"
     gene            1017..1799
                     /locus_tag="VGA15_16575"
     CDS             1017..1799
                     /locus_tag="VGA15_16575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589347.1"
                     /translation="MTERVTLITGASAGIGTELARVFASNGHRVVLVARRADRLSTLA
                     NEIVAGGGAAPIVIPCDLEQPDAGEKIAAALAAEGAEVEFVVNNAGFGLFGEAIELDL
                     AGQLGIIAVNIRTMTDLSLRFADSLIRNRGGILNVGSIAGFLPGPGMAVYYASKAYVL
                     SFSEALRRELAPRGVRVTTLCPGPVPSEFQVRAGFKPGFDSAVLNVCASDVAQAGYRG
                     LMANKRTVLPGFGIKMVPFLLRLFPRGFILAAVGRLQLHRRS"
     gene            1950..2750
                     /locus_tag="VGA15_16580"
     CDS             1950..2750
                     /locus_tag="VGA15_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX9589348.1"
                     /translation="MPFQSSKITPKTLLHFPGRVSTAPSAAEPLLPVLIVLHQETSSP
                     GRVGNALRALGHGLDIRRPRFGDPLPETLGGHAGAVIFGGPMSANDPDDFVRQEIDWI
                     AVPLREQRPFLGICLGAQMLAMQLGARVAPHPEGRAQIGYYPIRPTAAGLKICPDWPD
                     HVYHWHREGFELPASAELLAEGCDFPVEAFRFDQSFGFQFHPDVTYAMMHRWTTRGHA
                     RLELPGARARHHHFADRAVHDVTERAWLKAFLDGWLARVPAAMSEAAE"
     gene            2853..3362
                     /locus_tag="VGA15_16585"
     CDS             2853..3362
                     /locus_tag="VGA15_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9589349.1"
                     /translation="MTGDEFAGLLNEFTLSAESGDGARFASHFTDDAVYYDYIYGAHK
                     GRADIAHMMQDLFHRDAADYRWGMFDPVFDGTKGYAWSLSSFVSKIPQFKGQRVVIDG
                     ISRFVVRDGLVAEYRESVNGGVAMAQLGVEPDRMTKVFERWTGWLKERPETLDYLARP
                     KGSRAKSSV"
     gene            3423..4235
                     /locus_tag="VGA15_16590"
     CDS             3423..4235
                     /locus_tag="VGA15_16590"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9589350.1"
                     /translation="MAYQHILYEVSEKIATITLHRPDRMNAWTPIMERDVRHAMEAAA
                     AADDVRVIVLTGSGRAFCAGADMESLKGLDPGDIKRAESVPPFDMNRRPDWQTRYAYY
                     PSIPKPVIGMLNGATAGIGLVHALYCDLRFAADNTVFTTAFSRRGLIAEHGISWMLPR
                     IVGHANALDLLMSARRVQSDEALRIGLVNRLYPPDQLREQTYAYARDLADFVSPSAIA
                     VIKRQLYDAPFQTLAEATIDANREMLLALRGSDFREGVASFMEKRPPRFTGK"
     gene            complement(4466..4541)
                     /locus_tag="VGA15_16595"
     tRNA            complement(4466..4541)
                     /locus_tag="VGA15_16595"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4506..4508),aa:Ala,seq:cgc)
     gene            4730..5020
                     /locus_tag="VGA15_16600"
     CDS             4730..5020
                     /locus_tag="VGA15_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX9589351.1"
                     /translation="MRAILEVIMIVLDLYVWLLIASAILSWLIAFNVVNTRNQFVAAV
                     AEFLYRITEPVLAPIRSFMPNLGGLDISPIILILIIILIQKLVAYNLMPYFI"
     gene            5026..5358
                     /locus_tag="VGA15_16605"
     CDS             5026..5358
                     /locus_tag="VGA15_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX9589352.1"
                     /translation="MDPWRYSTQGISVALRVTPRGGRDDIDGLETLADGRIVVKVRVR
                     AIAEGGEANRAVMELLAKALGVPKAKVRILSGTTSRLKQIAVDGDPARLGDALRSLTA
                     AKPAKTKD"
     gene            5360..6244
                     /gene="folD"
                     /locus_tag="VGA15_16610"
     CDS             5360..6244
                     /gene="folD"
                     /locus_tag="VGA15_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX9589353.1"
                     /translation="MTARIIDGKVIASELRVRVADEVARVQREHGITPGLAVVLVGND
                     PASEVYVRGKHKQTQAAGMASFEHVLPADVAQNDLLALIEELNRDSSVHGILVQLPLP
                     KSLDTETIINAIDPAKDVDGLHPNNAGRLAGGFAALSPCTPLGCIILTKSVHASLEGM
                     NAIVIGRSNLVGRPLVQLLLNENATVTIAHSRSRDLAQLCARADLVYAAVGKPEMVRG
                     DWIKPGATVIDVGISRLPGVDGKTRLVGDVAFAEALNVAGAITPVPGGVGQMTVACLL
                     VNTLRAACVIHGLPKPAV"
     gene            complement(6442..7923)
                     /locus_tag="VGA15_16615"
     CDS             complement(6442..7923)
                     /locus_tag="VGA15_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HEX9589354.1"
                     /translation="MRRFIRIIRNILLLVIAAMVILAGVLTFNVVTHRSRQIEVFAVP
                     RAPIDRQGAATRLSEAIRFRTISNFLNPEQDAEALRGLQAHIAKSFPKFHATAKREIV
                     AGYSLLYAWEGSDAKAPPIALLAHQDVVPIAPGTEKDWQQQPFDGVIADGFIWGRGSW
                     DDKGNLYSMLEAAEAMARDGFRPKRTVYFAFGHDEEVAGVRGAKAIAATLASRGVRLD
                     FVLDEGLLIAEGLIKGLDKPAALIGVAEKGYATLVLTAHATPGHSSMPPRDTAIGMMS
                     AALARLEDNRLPMQIRGTVSEMFDTLAPEMSGFNRVALSNLWLFKPLLLREFEKSGPS
                     EATVRTTTALTIFQAGDKDNVLPGNAEATVNFRLIPGDTQASVTDHVRSTVNNDRISI
                     EPFPGNTDPPPVTGIASPSYRMLNRTIREIFPDVVVAPGLMVAATDSRHYAGIADNIF
                     RFSPVRANSEDLKRFHGTNERLSIEGYADMIRFYRRLIENTAG"
     gene            complement(8201..>8332)
                     /locus_tag="VGA15_16620"
     CDS             complement(8201..>8332)
                     /locus_tag="VGA15_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX9589355.1"
                     /translation="QIQHFFEHYKDLEPGKWVKVLRWGGAEDAHQLILEGIARAKAK"
BASE COUNT         1523 a         2588 c         2620 g         1601 t
ORIGIN      
        1 tccacatcac ggggccggtg atcgacgcgc ccgcggtagc cgccatcctg gacaaggcgg
       61 cgattacgcc cattcaaaag caggcggcgc tgcgcggcat cgccgacaag atcgtggttt
      121 atgagattcc ctgagattgc cgaaacgtaa tacgggctgg aaccggcatg gattgcgcag
      181 gctgagcttc cggcccatat attgacgaca ccacagtcgc cgatgcggcg gtcagctgaa
      241 cttgcctata ttcggtagat agatcgatgc tcgacggact acgccaatta atcaccgaca
      301 ttgtgtctcc cacggagcac caggatcggg cctttgacga taccggatat cgtctggcgg
      361 caacggcctt gctggttcac gtgatctcgc tcgacggcga gccgtccgag attgaaaaac
      421 gcaaacttca cagtctgctc gagaggcgtt tcgggctcga tcccggcacg gcagatcagt
      481 tgatcgcgtc tgcgatgctg gtcgagggtg aggcggtcga tctctatcat ttcaccagcg
      541 tcatcatgcg ctcggtgaat gaggagggca ggcttcgcat cgtcgaaatg atgtgggagc
      601 tggtctatgc cgacggccag gtcagcgagt tcgaggataa cgtggtctgg cgcgccgccg
      661 atctcttggg cgtgtcttcg cgcgatcgga tcgacctcaa gcatcgtgtc gccgccaagc
      721 tagccacgcc ggctaccgac aattcagata ttgccgaccc ggcaatgtga cacggcacgg
      781 tggaggagcc ccgatccgtc aaccgtgcgg taccggacat gacgaaattt taatgtgccc
      841 cgcgatagcc gctctcaact cgcgataagc cctgaaaata ttgctattcg ctgctgcagc
      901 actgtgcgtc cgacgacacc gatatgcttg cagatggctt gcatcccgcc taagccgtat
      961 gctctcgtcg tgagggttgg cggcctatcc cgcaattcat ctaagagcgc tgaatcgtga
     1021 ccgagcgtgt gacgctgatt accggtgcct cggcaggcat aggcaccgaa ctggcgcgcg
     1081 ttttcgcatc gaacggccat cgcgtcgtgc tggtggcgcg acgcgcggat cgtctgtcga
     1141 ccttggcgaa tgaaatcgtc gctggcggcg gcgccgcgcc gattgtaatc ccctgcgatc
     1201 tcgaacaacc cgatgccggc gaaaaaattg ccgcggcctt ggccgccgag ggggcggagg
     1261 tcgagttcgt cgtcaacaat gcaggcttcg gtttgttcgg cgaggcgatt gaactggacc
     1321 tcgccggaca gctcggcatc atcgcggtca acattcgcac gatgaccgat ttgtcgctgc
     1381 ggtttgccga cagcctgatc cggaatcgcg gcggcatcct caatgtcggc tcgattgccg
     1441 gctttctgcc ggggccggga atggcagtgt attatgcctc caaggcctat gtgctgtcgt
     1501 tctcggaagc gctgcgccgg gagctcgcgc cgcgcggcgt acgcgtgacg acgctttgtc
     1561 ccggcccggt gccgtcggaa tttcaggtcc gggcggggtt caaacccggt tttgattcgg
     1621 cggtcctgaa tgtctgcgct tcggatgtcg cgcaggccgg gtatcgcggg ctgatggcca
     1681 acaagcggac ggtactgccg ggcttcggca tcaagatggt gccgtttctg ctccggctgt
     1741 ttccgcgcgg gtttatcctg gcagcggtcg ggcgtcttca gctacacagg cgctcatgaa
     1801 ctggcgctca taaagcgcat cggcggcctg ttttggcccg cgatttgctt tgcagtcctg
     1861 aaagctcagg ccatttcatg tgaactcaca ccaaattaac gatcacttag gtatgattcc
     1921 ggcttgacag ccgccagcaa gaaaaatcga tgccgtttca gtcgagcaaa ataaccccca
     1981 agaccctgct gcactttccc ggccgcgtgt ccacggcgcc ttcggcagcg gagcctttgt
     2041 tgccggtgct gatcgtcctg caccaggaaa cctcgtcgcc gggccgggtc ggtaatgcgt
     2101 tgcgggcgct tgggcacggc ttggatatca ggcgtccgcg gtttggcgac ccgctcccgg
     2161 aaacgctggg cggccatgcc ggcgcggtca tcttcggcgg cccgatgagt gcgaacgacc
     2221 ccgacgattt cgttcgccag gagatcgact ggatcgccgt gccgctcagg gagcaacggc
     2281 cgttcctggg gatttgcctg ggcgcgcaaa tgctcgccat gcagctcggc gcgcgggtgg
     2341 cgcctcaccc ggagggccgt gcgcagattg gatactatcc gatccgcccc accgccgccg
     2401 gactcaagat ctgtccggat tggccggatc atgtctacca ctggcaccgc gagggatttg
     2461 aacttcccgc cagcgccgaa ttgctggccg agggctgcga tttcccggtt gaggcgtttc
     2521 gcttcgacca gtcgttcggc ttccagtttc atcccgacgt gacctacgcg atgatgcatc
     2581 gctggaccac gcgcggccat gcgcggctcg aactgccggg cgcccgggcg cgccatcacc
     2641 acttcgccga tcgcgcggtc catgacgtga ccgagcgggc ctggctgaag gcatttctcg
     2701 atggctggct ggcgcgcgtg ccggccgcca tgtcggaagc cgcggaatag cgcgtacggc
     2761 aacgccacgg cgatcctgaa agccttttct gttttacaaa tgtgatagtg tcggtcccac
     2821 aagcttgcgc cagccaggca acgggaggcg acatgaccgg cgacgaattc gcaggcctgc
     2881 tgaacgagtt cacattgtcg gcggaatccg gcgacggcgc gcgcttcgcc agccatttca
     2941 ccgatgacgc cgtttattac gactacattt atggcgcgca taagggccgg gcggacatcg
     3001 cgcatatgat gcaggatctg tttcatcgcg atgctgcgga ttatcgctgg gggatgttcg
     3061 atccggtgtt cgacggcacc aagggatatg cctggtcgtt gtcgagcttc gtctcgaaga
     3121 tcccgcaatt caaaggccag cgcgtggtga tcgacggcat cagccgcttc gtggtgcgcg
     3181 acggcctggt cgccgaatac cgcgaatccg tcaacggcgg cgtcgccatg gcacagctcg
     3241 gcgtcgagcc ggatcgcatg accaaagtat tcgaacgctg gaccggctgg cttaaagagc
     3301 ggccggaaac actggactat ctggcgcggc caaaaggttc gcgcgcgaaa agcagcgtct
     3361 gaaagcatcg atcaacaata acacgttgtc gcgaagacga tgacgaacac agggagatcg
     3421 cggtggccta tcagcatatt ctctatgaag taagcgagaa gatcgcgacc atcacgcttc
     3481 accggcccga ccgcatgaat gcgtggacgc cgatcatgga acgcgacgtg cgccacgcca
     3541 tggaagccgc ggccgcggcc gacgatgtgc gcgtcatcgt gctcaccggt tcggggcgcg
     3601 ctttctgtgc cggcgcggac atggaaagct tgaaggggct cgatcccggc gatatcaagc
     3661 gcgccgaaag cgtgccgccg ttcgatatga accgcaggcc ggactggcag acgcgctacg
     3721 cttactatcc gtcgataccc aaacccgtca tcggcatgct gaacggcgcc accgccggca
     3781 tcgggctggt gcacgcgctg tattgcgatc ttcgtttcgc cgccgacaac accgtgttca
     3841 ccaccgcgtt ctcgcgccgc gggctgatcg ccgagcatgg catcagctgg atgctgccgc
     3901 gcatcgtcgg tcacgccaat gcactcgatc ttttgatgtc cgcgcggcgg gtgcaaagcg
     3961 acgaggcgct gcgcatcggg ctcgtcaacc gcctgtatcc gcccgatcag ctgcgcgagc
     4021 agacctatgc gtatgcgcgc gatctggccg acttcgtctc gccgagcgcg atcgccgtca
     4081 tcaagcggca gctttacgat gcgccattcc agaccttggc cgaagcgacc atcgacgcca
     4141 accgggagat gctgttggcg ctgcgcggca gcgattttcg cgaaggcgtg gcgagtttca
     4201 tggaaaagcg gccgccaaga tttacgggga agtaacacca ggtgagttgc cagagcccgt
     4261 cgtcgccctt cgggctatgc cgggcacgct tcgctcttcg gtcttgcgcg gctgcgccac
     4321 gcgtagcccg aaggcgagac aattgaggtg tcggtgaaag atgttctgta gcgctccagc
     4381 ccgtcgtcgc ccttcgggct atgccgggca cgcttcgcct tcggtcttgc gcggctgcgc
     4441 cacgcgtagc ccgaagggcg aagcgtggtg gagccaggcg ggatcgaacc gccgacctcg
     4501 tcattgcgaa cgacgcgctc tcccagctga gctatggccc catcgcgacc gccaagggaa
     4561 atggcggcca gcaatccgcg ccatttacaa tccgcgccaa ggccaagtca agaacggcga
     4621 aatcgcggtt ttccgaggca tttgcccgga acttcccttg tttgcgggag atggaaccga
     4681 tatttagcaa gtagcctctt ccgcgaccga acccccgagt aagccagcca tgcgtgccat
     4741 tctcgaagtt atcatgatcg ttttggatct ctatgtctgg ctgttgatag cctcggccat
     4801 cctgtcctgg ctgattgcct tcaatgtcgt gaatacgcgc aatcagttcg tggcagcggt
     4861 cgcggagttt ctgtaccgga tcacggagcc ggtactggcg ccgatccgat cgttcatgcc
     4921 gaatctcggc gggctcgata tctcgccgat catattgatc ctaataatta tcctcattca
     4981 gaagctcgtt gcatacaatt tgatgcccta ctttatttag cgctgatgga tccttggcgc
     5041 tattccaccc aaggtatcag cgtcgcgctg cgggtcacgc cgcgcggcgg ccgtgacgac
     5101 atcgacggac ttgagacgct ggccgatggc cgcattgtgg taaaagtgcg cgtccgcgcc
     5161 atcgccgaag gcggcgaggc caatcgcgcg gtcatggagc ttttggcaaa agcgctcggc
     5221 gtgcccaagg ccaaggtgcg gattctatcc ggcacgacat cgcgcctgaa acaaatcgcc
     5281 gtcgatggcg atcccgccag gctcggcgac gcgctgcgaa gcctgaccgc cgcgaagccg
     5341 gcaaaaacaa aggattgaga tgacggcccg gatcatcgat ggaaaggtca ttgcatcaga
     5401 gcttcgcgtc cgtgttgccg atgaagtggc acgggtgcag cgcgagcacg gtattacgcc
     5461 cggccttgcg gtggtgctgg tcggcaacga ccccgccagc gaggtctatg tccgcggcaa
     5521 gcacaagcag acgcaggccg ccggcatggc gtcgttcgag cacgtgttgc cggccgacgt
     5581 cgcacagaat gatctgctgg cgctgatcga agagctcaat cgcgattcga gcgtgcacgg
     5641 catcctggtg cagctgccgc tgccgaaatc gcttgatacc gaaaccatca tcaacgccat
     5701 cgatccggcc aaggatgtcg acgggctgca tcccaacaac gccggacgcc tggccggcgg
     5761 atttgccgcg ctgtcgccgt gcacgccgct ggggtgcatt atcctcacca agagcgtgca
     5821 tgcgtcgctc gaaggcatga acgccatcgt catcggccgc tccaatctgg tcggcaggcc
     5881 gctggtgcaa ttgctgctca acgaaaacgc cacggtaacg atcgcgcatt cgcgctcgcg
     5941 cgatctcgcg caactctgcg cccgcgccga tctggtttac gccgccgtcg gcaagcccga
     6001 aatggtgcgc ggcgactgga tcaagccggg cgccacggtg atcgatgtcg gcatcagccg
     6061 gctgccgggc gttgacggaa agacccgtct ggtcggcgat gtcgcgtttg ccgaggcgtt
     6121 gaatgtcgcg ggcgcgatca caccggtgcc cggcggcgtc ggccagatga cggtggcgtg
     6181 cctcctggtc aacacgctgc gcgccgcctg cgtcattcac gggttgccca agccagcggt
     6241 gtgacgtagc gaagggccac gtgtcccgga cgcggtgcag cgtcctttac ggtgcaccgc
     6301 agagccggga cccacagcta cgcggttacg gagcacggac cccggatcag cagcgcaccg
     6361 cccatagcgc gtcgaagacg cgcgtaaacg cgctgatggc gctgcgcagc atccggggaa
     6421 cggcctccga tgcagatcaa gtcaccccgc cgtattctcg atcagccgcc gatagaaccg
     6481 gatcatgtcg gcatagcctt cgatgctgag gcgctcgttg gtgccgtgaa accgtttcag
     6541 gtcttccgag ttggcgcgca ccggcgaaaa ccggaagatg ttgtcggcaa tcccggcgta
     6601 gtggcgcgag tcggtcgccg ccaccatcag cccgggcgcg accacgacgt cgggaaaaat
     6661 ctcgcggatg gtccggttga gcatgcggta ggagggactc gcgatgcccg tcaccggcgg
     6721 cggatcggta ttgccgggaa acggttcgat cgagatacga tcgttattga cggtagagcg
     6781 aacatgatcg gtcacgctcg cctgcgtatc gcccgggatc aggcggaagt tgacggtggc
     6841 ctcggcgttg ccgggcagca cattgtcctt gtcgccggct tggaagatcg tcagcgcggt
     6901 cgtggtgcgg accgtcgctt cgctcggtcc gctcttttcg aattcgcgca acaataacgg
     6961 cttgaacagc cagagattgg acagcgcgac gcggttgaag ccgctcatct ccggcgccag
     7021 cgtgtcgaac atttccgaga cggtgccgcg aatctgcatc ggcagccgat tgtcttcgag
     7081 ccgcgccaac gccgcgctca tcatgccgat cgcggtatcg cgcggcggca tcgacgaatg
     7141 gccgggcgtc gcatgcgccg tcaaaaccag cgtggcatag cctttttcgg cgacgccgat
     7201 caatgcggct ggcttgtcga ggcccttaat gaggccttcc gcgatcaaca ggccttcatc
     7261 gagcacgaag tcgagccgga cgccgcgcga agccagggta gcggcgattg cctttgcgcc
     7321 ccggacgccg gcgacttcct cgtcatggcc gaacgcgaaa tagacggtcc gcttgggacg
     7381 aaatccatcg cgagccatcg cttcggcggc ctccagcatc gaatacaggt tgcccttgtc
     7441 gtcccacgat ccgcggcccc agataaaacc gtcagcgatg acgccatcga acggctgctg
     7501 ctgccagtct ttctcggtgc ccggcgcgat cggcaccacg tcctgatgcg ccaacagggc
     7561 gatcggcggc gccttggcgt cagagccctc ccaagcgtag agcaggctgt agcccgcaac
     7621 gatctcgcgc ttcgccgtgg catgaaattt cggaaaactt ttggcgatgt gcgcctgcaa
     7681 gccgcgcagc gcttcggcgt cctgttccgg attgagaaaa ttcgagatcg tccggaaacg
     7741 gatcgcctcg ctgagccgcg ttgccgcgcc ttgccggtcg atcggcgcac gcggcaccgc
     7801 gaaaacctca atctgccgcg agcggtgcgt caccacattg aacgtcagca cgccggcgag
     7861 gatcaccatc gcagcgatca cgagcagcag gatgttgcgg atgattctta tgaagcggcg
     7921 catgcggtga tctcaaatcc tcgtgccccg gatgcagcgc agcgccatca gcgcgttcac
     7981 gcgcgtcttc gacgcgctca taagcgcgtt cacgcgcgtc ttcgacgcgc tatgggcggt
     8041 gcgctgctga tccgggctcc atcttagtgc gaggtaggtc ccggctctgc ggcgcatcgt
     8101 gaccggacga tgcggagcct gtcatcgggc gcgcattcgc gcgacccgtt ggcatcgccg
     8161 ggagaacgat gcaccgcgtc cgggacacgg acttcgaagc ctactttgcc ttggcgcgcg
     8221 cgataccttc gagaatcaat tgatgcgcat cctcggcgcc gccccaccgc agtactttca
     8281 cccatttgcc gggttcgaga tccttgtagt gctcgaagaa gtgctggatc tg
//
LOCUS       DASWGM010000584         8817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1010913,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000584 DASWGM010000000
VERSION     DASWGM010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010913"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1204)
                     /locus_tag="VGA15_16625"
     CDS             complement(35..1204)
                     /locus_tag="VGA15_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9589356.1"
                     /translation="MFNFHSFCDLRPLVVLLAVTLSGCGEKPPPQAAAGPPPVTVAQP
                     TKRTVTDWDEFTGRFEAVEEVQVRARVGGFVTNVEFRDGAFVNTGDLLYIIDSRPFEA
                     VAEQADGQLSDARAKGELAKRELDRALTLNQTQAVSDSIVDQRRQALQAAKAAEMQAA
                     GALKAAQLNIEFAHVIAPIGGRVSRHLISVGNLVQGSDAGGGTLLTSIVSLDPIYVYF
                     DMDEATYLKNNRLYFEGKRPSSRENPNPVQVSLTGETKPSHDGKVDFLDNRLDLSTGT
                     LRGRAVIPNKDYSILPGQFGRVRLIGSAPYEALLLPDTAIATDQSRKIVFVVKDDDTV
                     EAKPVVLGPLDEGLRVIREGLKAEDRVIVDGLQRARVGAKVSPHAAPAAPAGSKT"
     gene            1685..3463
                     /locus_tag="VGA15_16630"
     CDS             1685..3463
                     /locus_tag="VGA15_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9589357.1"
                     /translation="MDALPSQKSFAEAQNPARGADHIAPDCAGANFYAIDRGLRDLLE
                     LYLAPDDFHRLEPHFDRLGALVGGRLGELARAADKHPPVLNARDRFGRDEDWIDYHSS
                     YREMEKIAFGDFQFHAVSHRAGTLGMDRPLPAVAKYALQYLFVQAEFGLMCPISVTDT
                     SIHLIRKFASAELQDYLLPKMLSGDIATLWKGTQFMTERSGGSDVGAIETIARCVDGE
                     WRLYGDKWFCSHADADVALLLARPEGAASGTRGLALFALPRRLKNGRRNAYRIVRLKD
                     KLGTRSMASGEILLEGAVAYLIGEADRGLKQMMEQVNLSRLSHGVRAAAMMRRGVNEA
                     MVCARGRVAFGKAIIEYPLLRRQLLKITVPTEQALSIFLFAANAMDRANAGSEQAESL
                     LRILTPLLKFRACRDNIPVATGSMEVRGGNGYIEEWVNARLVRDAHIGVLWEGTSNIN
                     ALDIVTRAVGKSRAHRTLEAALRKLLDEATPIPAAFRDRLRLALDKTIVFAERVAAEP
                     ALEATARQAASALYHVTSTVLMTWEAAQPAADARRALYARFVLEHRLSPQDPLEPRVG
                     DWERDAAQIVFSEHRVALAEIAGLLN"
     gene            complement(3610..4608)
                     /locus_tag="VGA15_16635"
     CDS             complement(3610..4608)
                     /locus_tag="VGA15_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9589358.1"
                     /translation="MSTAGPNESHSAPTLSAVARLAGVSSITVSRVVRLPGLVAPETR
                     GRVEAAMRELGYVPNQVAGSLASARTSSVGVLVPTIANSIFADTVQGLSDELEPLGYA
                     VILAQSRYDSAREDHMLSALLSRRPEAIIMVGSPATDDGARLLRRAGIPVAETWDLPA
                     DPIDAVAGFDNYAAGAAVARHLVAQGRQSLAFIGGDDPRATRRWHGFKDAALQAGAKI
                     PRRLVLERNASGSVAALANLPGVDAVFAANDAHAIGFMSGLRAAGLLRNGPASEQPVA
                     VIGLGDLEMGRLIAPSLSTIRVHGDAIGRTAAKLMLTREGPRHVDLGFELVLRDSG"
     gene            4793..5662
                     /locus_tag="VGA15_16640"
     CDS             4793..5662
                     /locus_tag="VGA15_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX9589359.1"
                     /translation="MISEQDVQAYKRDGVIVVPEILGADTLTQLRSVIAELVAGAAKT
                     LEHTDVYDLEPGHTAESPRVRRIKTPHKVHALFDQIVRSPAVLDILKKLIGPGLRLHG
                     SKLNMKSAHYGSPVEWHQDWAFYPHTNDDILAIGVLLDDTDLSNGPMLVTPGTHTGEV
                     WNHHGDDGCFAGLIDPDTIRNEIERAVPCMGRAGSMSFHHVRALHGSAMNTSDRPRNL
                     LLYEVAASDAWPLMGVKDFAEFNSRLLTGDPVVAPRMTNVPVRLPLPPATRQGSIYET
                     QSAAKKSYFTRAA"
     gene            5741..7093
                     /locus_tag="VGA15_16645"
     CDS             5741..7093
                     /locus_tag="VGA15_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9589360.1"
                     /translation="MTDIIADSTGRSRLRVILAASIGSALEWYDFFLYGTAAALVFGD
                     LFFPKSDPVVGTLLAFLTFGVGFVVRPLGGILFGIMGDRFGRKPVLVATLLMIGIGTT
                     AIGVLPTYAQIGYWAPALLVLLRVIQGLGAGAEYGGAVIFLVENAPAQHRGFWGSFAP
                     LGVSIGNLLAAGAFALVTLLPREDLMSWGWRLPFLASFLLILVGIFVRLRIAETPVFT
                     EAVVARGKIERNPAMEALRRHPRNFMVVLGARLAENGLGYLFPVFGLSYVINTLGVPK
                     ADALSALMLAFVVELFTIVGFATLSDRIGRRPVYMFGALAGVAFAFPFFLLVGTKEWI
                     WIALAFIVARAVVTAAMFGPQAAYFAELFPPQRRFAGFAFARELGSLLAGGPAPFLAA
                     ALVAWSGSWWPVACYAAGLSALTALAIWAGPETYQESITVDNTSDGAMHAVAAMPQRA
                     "
     gene            7109..7981
                     /locus_tag="VGA15_16650"
     CDS             7109..7981
                     /locus_tag="VGA15_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX9589361.1"
                     /translation="MAQQLRILQSLWAMERRRADQPELPLQTQLAMIRDAGFDGAGVR
                     FVDAAFATEATSFLRAHGMIWQAQCYPTSVDDLKPVLELVARCGADHVNLQPNVRPQR
                     IEDCIPLLDGWRRLAEQAGVAVHIETHRDRMTTDLFFTLQLLDCFPDLRLTADVSHYL
                     VGREFAWPVDEVNHALIKRILDNSWGIHGRIASREQVQISLGFPQHQGWVELFMGWWE
                     YAIRSWRKRAKPDAVLAFLCELGPPPYAITGPDGAELSDRWQDALKMKDMVRALWQRI
                     VDEEARGIEQRRAM"
     gene            complement(8168..>8817)
                     /locus_tag="VGA15_16655"
     CDS             complement(8168..>8817)
                     /locus_tag="VGA15_16655"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9589362.1"
                     /translation="IGCILLEPDTPGFEVTGTYHTMGGENLHEIQFNNCELPLENLVI
                     RDDGFRKLLNAFNTQRCLNPSISLGLAEGAFEEARNYVRDRTVFGKAIGDYQGVRWKL
                     AEMYREIETARSILYRACITANPFPDPFMAAVAKITCNEMSNRVTSEAIQLHGGFGFT
                     DEFAVSRLYRAARYGTLGGGTSETLRDLIGKRIMDAAETGAELLAYDMNGADLAV"
BASE COUNT         1571 a         2769 c         2824 g         1653 t
ORIGIN      
        1 aggatcggcc gattgatgga aagctgaccg aggttcatgt cttgctaccg gcaggagcag
       61 cgggcgcggc gtgtgggctc accttggcgc cgacgcgcgc acgctgcagg ccatcgacaa
      121 tcacgcggtc ttccgccttc aagccttcgc ggatcacgcg caagccctcg tcgaggggtc
      181 cgaggacaac gggttttgcc tccacggtgt cgtcatcctt gaccacgaac acgatcttgc
      241 gcgattggtc ggtggcgatg gcggtgtccg gcaacagcag cgcttcgtaa ggcgcgcttc
      301 cgatcagccg cacccggccg aactgcccgg gcagaatcga ataatccttg ttcgggatca
      361 ccgcccgccc gcgcagcgtg ccggtcgaga ggtccagccg gttgtcgagg aaatcaacct
      421 tgccgtcatg tgacggtttg gtttcgccgg tcagactgac ctggaccggg tttggattct
      481 cgcgcgaact cggacgcttg ccttcgaaat agagccgatt gttcttgaga taggtcgcct
      541 cgtccatgtc gaagtagacg tagatcgggt cgagcgagac gatcgaggtc agaagcgtgc
      601 cgccgccggc gtcgctgccc tgcacgagat tgccgacgct gatgagatgg cggctcactc
      661 tgccgccgat cggggctatg acgtgggcaa attcgatgtt gagctgggct gccttcagcg
      721 cgcctgcggc ctgcatctcc gcggcctttg ccgcctgcag ggcctggcgg cgctgatcga
      781 caatggagtc cgagaccgcc tgggtttgat tcaatgtgag cgcgcggtcg agttcgcgtt
      841 ttgccagttc ccccttggcg cgggcatccg agagctggcc atcggcctgc tcggcgacgg
      901 cctcgaaggg ccgggaatca atgatgtaca agagatcgcc ggtattgacg aaggcgccgt
      961 cacgaaactc gacattggtg acgaatccac cgacccgggc gcgaacctga acctcttcca
     1021 ccgcctcgaa ccgtccggtg aattcgtccc aatcggtgac ggtccgtttg gtgggctgcg
     1081 cgaccgtgac cggcggcgga cctgccgcgg cctgcggcgg cggcttttcc ccgcagccgg
     1141 acagggtcac cgcgagcagc acgaccagcg gtctgaggtc gcagaacgaa tggaaattga
     1201 aaatttgtat ttgagctaaa cgctccgatt tctcagtcgc ggccaattcc atcccctcca
     1261 cactttgccg ccatcaaggc ggccgtcatg atccgcggca ggaccaagat gagaccggct
     1321 gccagtctgt tcaagagccg gccgggatca agcaaaatta tcatgcgctg cgcacgcggg
     1381 cgagcgtcat gaaggtattc tgcatcatcg gcgccagtgt gacggtccgt tagccgagca
     1441 gcccggccag atggctaggc tgcgtccacg tgttccgggt gcagcgttga tcgcaatccg
     1501 gtgttcagag attgggaaat tgctcgacat ttccaggttc aatcaattcg tacgatccat
     1561 agagccccgt gaaccctgtt gtggaatttc acaacccgct gccgctacac caatagtgtt
     1621 ggaatattcc gctggaaaac gatattgagc gtcgatccgc cgatccgtaa aacgagggtc
     1681 agacatggac gcgctgccat cgcaaaaatc gttcgccgaa gcgcaaaatc ccgcgcgcgg
     1741 cgccgatcac attgcgccgg attgtgcagg tgcgaatttc tacgcgatcg atcgcggcct
     1801 gcgcgatctt ctagagctgt atcttgcgcc agatgatttt caccggctgg aaccgcattt
     1861 tgatcgcctc ggcgcgttgg tgggtggcag gctcggtgaa ctggcgaggg ccgccgacaa
     1921 gcatccgccg gtcttgaatg cacgcgatcg gttcggccgc gacgaggact ggatcgatta
     1981 tcattcgtcc taccgcgaaa tggagaaaat cgcgttcggc gatttccagt tccacgccgt
     2041 gagccatcgc gccggcacgc ttgggatgga tcgcccgctg cccgcggtgg ccaaatatgc
     2101 gctgcagtat ctgttcgtgc aggccgagtt cggactgatg tgtccgatca gcgtgaccga
     2161 cacctcgatc catctcattc gcaagttcgc cagcgccgag ctccaagact atctgctgcc
     2221 gaaaatgctg tcgggcgata tcgccacgct atggaagggc acgcagttca tgaccgaacg
     2281 ttccggcgga tccgacgtcg gcgcgatcga aaccatagcg cgctgcgtgg acggggagtg
     2341 gcggctgtat ggcgacaaat ggttctgctc gcacgccgac gcggatgtag cgctgctgct
     2401 ggcgcgcccg gaaggcgcag cttctggtac caggggactg gcgctgttcg ccctgccgcg
     2461 ccggctgaag aacggccgcc gcaacgccta tcgcatcgtt cgcctcaagg acaagctggg
     2521 cacccgctcg atggcgagcg gcgagatcct tttggaaggc gcggtcgcct atctcatcgg
     2581 tgaggccgac cgcggcctca agcagatgat ggagcaggtc aacctgtcgc ggctttcgca
     2641 cggcgttcgg gctgccgcca tgatgcggcg cggcgtcaac gaggcgatgg tttgcgcgcg
     2701 cggtcgcgtt gccttcggca aggccattat cgaatatccc ctgctccgcc gccagctctt
     2761 gaagatcacc gtgccgaccg agcaggcgct ctcgatcttc ctgtttgccg ccaacgccat
     2821 ggatcgcgcc aatgccggtt cggagcaagc agaaagtcta ttgcgaattc tcaccccgct
     2881 gctcaaattc cgcgcctgcc gcgacaatat tccggtggcc acgggttcga tggaagtccg
     2941 cggcggcaac ggttacatcg aggagtgggt gaatgcgcga ctggtccgcg acgcccatat
     3001 cggcgtgctc tgggaaggca ccagcaacat caacgcgctc gatatcgtca cccgcgcggt
     3061 cggcaagagc cgtgcccacc ggacgctgga agccgcgctc aggaaattgc tggatgaggc
     3121 cacgccgatt ccggcggcct tccgggaccg tttgcggctg gcactcgata agacaattgt
     3181 gttcgccgag cgcgtcgcag ccgagcccgc actggaagcg accgcgcggc aggcggcaag
     3241 tgcgctctat catgtcacca gcaccgtact catgacatgg gaagcggccc agccggcggc
     3301 ggacgcgcgc cgggcgctgt atgcgaggtt cgtgctcgaa catcgcctga gcccgcaaga
     3361 tccgctggag cctcgcgtgg gcgactggga gcgcgatgcg gcgcagatcg tattctcgga
     3421 gcacagagtt gcgctggcgg agatcgccgg gctgttaaat tagagcgcat tccgctcgcc
     3481 acacaacacc tgtcatcgcc cggcttgacc gggcgatcca gtacgccgcg gcctttccgg
     3541 ttgaccacta tcgcctctgg aatactgggt cgcccggtca agccgggcga tgacggattt
     3601 ttgtccaact cagccgctat cgcgcagcac cagctcaaag ccgagatcga catggcgcgg
     3661 tccttcacgc gtcagcatca gctttgcggc cgtgcggccg atggcgtcgc catgcacgcg
     3721 gatggtgctc agactgggcg cgatcagccg gcccatttcc agatcgccca gaccaatgac
     3781 ggcgacgggc tgctctgagg ctggaccatt tcgtaacagc ccggcggcgc gcaggcccga
     3841 catgaagccg atcgcatgcg catcattggc ggcgaacacc gcgtccacgc cgggcaggtt
     3901 ggcgagcgca gcaacactgc ccgaggcgtt gcgctccaga accagccggc gcggaatttt
     3961 ggcgccggct tgcagcgcgg catccttgaa tccatgccag cggcgcgtgg cccggggatc
     4021 gtcaccgccg atgaacgcca ggctctgacg gccttgcgcc accagatgcc gtgcgaccgc
     4081 ggcccccgcg gcgtaattgt cgaaaccggc gacggcatcg atcgggtcag ccggaagatc
     4141 ccaggtctcg gcgacgggaa taccggcgcg ccgcagcagc cgggcgccgt cgtcggtcgc
     4201 gggcgagccg accatgatga tcgcctcggg gcgccgcgac agcagcgctg aaagcatatg
     4261 atcttccctt gccgagtcgt aacgcgactg ggccaggatc acggcgtagc cgagcggctc
     4321 caattcgtcg gacaggccct gcacagtatc ggcgaagatc gaattggcga tggtgggcac
     4381 cagcacgccg accgagctgg tgcgggcact tgccagggag cctgccacct ggttcggcac
     4441 atagcccagt tcccgcatcg cggcctcgac ccggccccgg gtctccggtg ccacaaggcc
     4501 cggcaaacgc accacgcggc tgacggtgat ggacgatacg cccgcgagcc tcgccaccgc
     4561 ggacagggtt ggggcggaat gcgactcgtt cggtccggca gtgctcatgc caaaagacta
     4621 ccgaaatccc cgccggaata catgatttca gcttgcgcca gataaatgac agcgctatca
     4681 ttttcatgca accgctgttt catcgcatga tagcgctatc atgacgggaa cgccggcggg
     4741 agagaatccg cgtcgagaag cgggtcggga agccaagaag gagagaagct ggatgatttc
     4801 agaacaggat gtgcaggcct acaagcgcga cggggtgatc gtggtgcccg aaatattggg
     4861 cgcagatacg ctcacccagc tgcgctcggt gatcgcggag ctggtcgcgg gggcggcaaa
     4921 aaccctcgag cacaccgatg tctatgacct cgaaccgggt cacaccgcgg aaagcccgcg
     4981 cgtccgccgg atcaagacac cgcacaaggt gcacgccttg ttcgaccaga tcgtccggtc
     5041 gccggccgta ctcgacatcc tcaaaaagct gatcggaccc ggcttgaggc tgcacggctc
     5101 aaaactcaac atgaagtcgg cgcattacgg atcgccggtg gaatggcatc aggactgggc
     5161 gttctatccg cacaccaatg acgacattct cgcgatcggc gtgctgctcg acgataccga
     5221 tctgtcgaac gggccgatgc tggtcacgcc gggcacgcat accggcgagg tctggaatca
     5281 ccatggcgat gacggctgtt ttgccggatt gatcgatccc gacaccatcc gaaacgagat
     5341 cgaacgcgcg gtgccttgca tgggacgggc cggcagcatg tcgttccatc atgtccgggc
     5401 gttgcacgga tcggccatga acacgtcgga ccggccgcgc aacctgttgc tgtacgaggt
     5461 ggcggcgagc gacgcctggc cgctgatggg cgtgaaggat tttgccgaat tcaacagccg
     5521 gttgctgacc ggcgaccccg tggtggcgcc gcggatgacc aatgttcccg tgcgccttcc
     5581 gctgccccct gcgacgcggc agggatcgat ttacgaaacg cagtccgcgg cgaagaaatc
     5641 ctacttcacc cgcgcggcgt gatcttgcga cggcggctgc gccaaggcgc agctgccgac
     5701 taccaacgat aaagttacaa aaaacaaaac gggggagacc atgacggaca tcatagccga
     5761 tagcaccgga cgttcgcgcc tgcgggtgat ccttgccgcc agtatcggat ccgcgctcga
     5821 gtggtatgac ttcttcctct acggcaccgc cgcggcactg gtgttcgggg atctgttctt
     5881 tccaaagagc gatccggtgg tcggcacgct gctggcgttc ctgaccttcg gcgtcggttt
     5941 cgtggtgcga ccgcttggcg gcatcctgtt cggcatcatg ggcgaccggt tcgggcgcaa
     6001 gccggtgctg gtggcgacac tgctgatgat cggcatcggc accaccgcga tcggtgtgtt
     6061 gccgacctat gcccagatcg gctattgggc gccggcgcta ttggtcttgc tgcgtgtcat
     6121 tcaaggattg ggagccggcg ccgaatatgg cggcgcggtg atcttcctcg tggagaacgc
     6181 cccggcacag catcgcggtt tctggggcag cttcgcaccg ctcggcgtgt cgatcggcaa
     6241 tctgctcgca gccggtgctt tcgcgctggt gacgctattg ccgcgcgaag acctgatgtc
     6301 gtggggttgg cgtctgccgt ttctggcaag cttcctgctc attctcgtcg gcattttcgt
     6361 ccgtcttcgc atcgcggaaa cgcctgtttt caccgaggcg gtggtggcgc gtggcaagat
     6421 cgagcgcaat ccggcgatgg aagcgctgcg ccggcatccg cgtaatttca tggtggtgct
     6481 cggcgcgcgg ctggccgaaa acggcctcgg ctatctgttt ccggtgttcg gcctcagcta
     6541 tgtcatcaac acgctggggg tgccaaaggc cgatgccctg agcgcgctga tgctcgcctt
     6601 cgtggtcgaa ctgttcacca ttgtcggttt cgccacgctc tccgaccgga tcggacggcg
     6661 cccggtctat atgttcggcg cgctggccgg cgtggccttc gcgttcccgt tcttcctgct
     6721 ggtcggcacc aaggaatgga tctggatcgc gctggccttc atcgtggcgc gggcggtggt
     6781 gacggcggcg atgttcggcc cgcaggccgc ctatttcgcc gaactgtttc cgccgcagcg
     6841 gcgttttgcc ggcttcgcct tcgcccgcga actgggctcg ctgctggccg gcggtccggc
     6901 gccgttcctg gccgcggccc tggtggcatg gtcgggaagc tggtggccgg tcgcctgcta
     6961 tgccgctggt ctgtccgcac tcacggcgct ggcgatctgg gccggccccg agacctatca
     7021 ggaaagcatc acggtcgaca atacgtcgga tggcgcgatg cacgcggtgg cggccatgcc
     7081 acagcgagcc taggccaagc cctaagccat ggcgcagcaa cttcgcatcc tgcaatcgct
     7141 ctgggcgatg gagcggcggc gggcggacca acccgagttg ccgctgcaga cccagctcgc
     7201 gatgatccgc gacgccggtt tcgatggtgc cggcgtccgc ttcgtcgatg ctgctttcgc
     7261 caccgaagcg acctccttcc tgcgcgcgca cggcatgatc tggcaggcgc agtgctaccc
     7321 gaccagcgtc gatgatctga aaccggtgct ggaactggtg gcgcggtgtg gggcggacca
     7381 cgtcaatctg cagccgaacg tgcggccgca gcggatcgag gattgcattc ccctgctcga
     7441 cggatggcgt cggctggccg agcaggccgg tgtcgcggta catatcgaaa cccaccgcga
     7501 ccgcatgacc acggatctgt ttttcacgct gcaactgctg gattgctttc ctgacctgcg
     7561 gctgacggcc gacgtttcgc attacctggt gggccgcgaa ttcgcctggc cggtcgacga
     7621 ggtcaatcac gccctgatca agcgcattct cgataacagc tggggcattc atgggcgaat
     7681 cgccagccgc gagcaggtgc agatttcgct cggctttccg caacaccagg gctgggtcga
     7741 attgttcatg ggatggtggg aatacgccat ccgctcctgg cgaaaacgcg cgaaacccga
     7801 tgccgtgctg gccttcctgt gcgaactcgg cccgcccccc tacgccatca ccggccctga
     7861 cggcgccgag ctatcggacc gctggcaaga cgcgttgaaa atgaaagaca tggtccgggc
     7921 gctgtggcag cgcatcgtcg acgaggaggc gcgtggcatc gagcaaagac gtgcgatgtg
     7981 agactccact ccgtcatccc gggttcgact gcggagcctg tcatcgggcc gcgccttgcc
     8041 cgtcgtcggt cgcgcattcg cgcgacccgt tggcgcgaac ccgttgggga aacccggccc
     8101 tgacattcgg cgacgatgag acgctcggaa cgatcgtgcg gtcttccgcg cgacaccatt
     8161 tggattgtca taccgcgaga tcagcgccgt tcatgtcata agccaaaagc tcagcgcccg
     8221 tctccgccgc atccatgatc cgcttgccga tcagatcacg gagcgtctcg gaggtgccgc
     8281 cgccgagcgt gccgtagcgc gctgcgcgat agagacgcga taccgcgaat tcgtcggtga
     8341 aaccgaatcc gccgtgcaac tggatcgcct cgctcgtgac ccgattggac atctcgttgc
     8401 acgtgatctt ggccaccgcc gccatgaaag gatcgggaaa cggattggca gtgatacagg
     8461 cgcgatagag gatgctgcgg gccgtctcga tctcgcggta catctcggcg agtttccagc
     8521 gcacgccttg atagtccccg atcgcctttc cgaacaccgt tcggtccctg acatagttgc
     8581 gggcttcttc aaaagcgccc tcggccagcc caagcgatat actcggattg aggcagcgct
     8641 gcgtgttgaa ggcgttcagc aactttcgaa agccgtcgtc gcgaatcaca agattctcca
     8701 gcggcaattc gcaattgttg aactggatct cgtggaggtt ctcgccgccc atcgtgtggt
     8761 aggtcccggt cacctcgaaa cccggcgtgt cgggctccag cagaatgcag ccgatac
//
LOCUS       DASWGM010000585         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1288379,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000585 DASWGM010000000
VERSION     DASWGM010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288379"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="lpxB"
                     /locus_tag="VGA15_16660"
     CDS             complement(<1..945)
                     /gene="lpxB"
                     /locus_tag="VGA15_16660"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX9589363.1"
                     /translation="MPARTSGSVTRKIFLIATEESGDRLGSSLMKVLRQRLGGAVRFE
                     GVGGQSMAREGLVSLFPIEELSIMGLAAVVKQLPKILRLIRETATAVTEASPDILVII
                     DSPDFTHRVARRVRARDPSIPIIDYVSPSVWAWRPGRARAMRRYVDHVLGLLPFEPEA
                     YRRLRGPPCSYVGHPLIEQIGQLRPGADEQMRREEQPPVLLVLPGSRSSEINHHMAVF
                     GETLGLLHAQDVVFELILPTMPHLQEAVAQAVRSWPVQPRIVVREKEKRAAFRIAHAA
                     FVKSGTATLELAIAGVPMVAAYKVGAVEAAIMRRAIRTS"
     gene            complement(945..1802)
                     /gene="lpxI"
                     /locus_tag="VGA15_16665"
     CDS             complement(945..1802)
                     /gene="lpxI"
                     /locus_tag="VGA15_16665"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610857.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX9589364.1"
                     /translation="MTNEVMQISSPIGLIAAGGVMPFAVADSLIARGLDPVVFALKGA
                     CDPVRVARFRHHWISVGQVGRAVKLFRSENCRDLVFIGTLVRPALSEIRLDWGTLRVL
                     GRVWAAFRGGDDHLLSGIGRILEQDGFRMVGIRDVAPDLLMPEGCITQVTPDQSAAAD
                     IARGRDVLRALSPFDIGQAAIVVDGHVVGVEDIEGTDGLLARVARLRAEGRIRAKAAR
                     GVLVKAPKSAQDLRFDLPTIGPRTIEGAGRAELAGVAVVAGNTIVVEPQTMIEAADAA
                     GLFVTGLSA"
     gene            complement(1819..2622)
                     /gene="lpxA"
                     /locus_tag="VGA15_16670"
     CDS             complement(1819..2622)
                     /gene="lpxA"
                     /locus_tag="VGA15_16670"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX9589365.1"
                     /translation="MSTIDPTARVEDGAVIGEGTSIGPYCVVGPHVVIGANCKLIAHV
                     HITAQTTIGDGCTIYPFVSLGTPPQSLSYRGELTGLAIGQGCTIREQVTMNAGTMAGG
                     GITRVGERGYFMNCSHVGHDCIVGNDVIFATSATLGGHAEIGDFVFIGGLSAVHQFTR
                     VGAQVMVGALTGVRSDVIPFTLASGQFAALTGLNTVGLKRRKFTRERLAVVRLFYQQL
                     FHGSGVFADRLNEVRHLAAADPAIAEILTFIDAAKHRELCQPVKDGKRS"
     gene            complement(2741..3199)
                     /gene="fabZ"
                     /locus_tag="VGA15_16675"
     CDS             complement(2741..3199)
                     /gene="fabZ"
                     /locus_tag="VGA15_16675"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX9589366.1"
                     /translation="MTDAPVRFELVDINAILKTLPHRYPMLLIDRVINIRSDYSGIGI
                     KNVTFNEPAFQGHFPERPVYPGVMMIEAMAQTAGVIGILSVEGTEKPRAVYFLTIDKC
                     KFRKPVLPGDTVEYHMRSIGRRKTMWWFHGNATVNGVTVAEADVGAMLTD"
     gene            complement(3196..>4014)
                     /gene="lpxD"
                     /locus_tag="VGA15_16680"
     CDS             complement(3196..>4014)
                     /gene="lpxD"
                     /locus_tag="VGA15_16680"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX9589367.1"
                     /translation="HVAVLRAAHPFRDFVKIARVLHGDALRPQSWFGNAGIAPSAVID
                     PTAHLEDGVIVDPLAVIGPNVEIGAGTIIGSGAVIGVNVRIGRDCNVGSHTAIQCALI
                     GNNVLIHPGCSIGQDGYGFVFFGPDGHLKVPQTGRVLIQNDVEIGAGTTIDRGSLRDT
                     VIGEGTKIDNQVQIGHNVTIGRHCLLAAQIGLAGSLTIGDNVALGAKVGINNHLHIGD
                     GAQVTAMSAVKDDIPANGRWGGYFAKPTKQWFREIIAVERLVRDGTADRGEGRE"
BASE COUNT          772 a         1330 c         1197 g          715 t
ORIGIN      
        1 actggtgcgg atcgcccgcc gcatgattgc tgcctcgact gcgccaacct tgtaagcggc
       61 gaccatcggc acgcctgcga tcgcaagctc gagcgtcgcg gtacccgatt tgacgaaagc
      121 cgcatgagcg atacgaaatg ccgctcgctt ctccttttcc ctgacgacaa tgcgcggctg
      181 caccggccag cttctcacag cctgcgcgac tgcctcctgc aaatgcggca tggtaggaag
      241 gatcagttcg aacacgacgt cctgcgcgtg cagcaggccc agcgtctcgc cgaataccgc
      301 catgtgatgg ttgatctcgc tgctgcggct tcccggcagc accagaagaa ccggcggctg
      361 ttcctcccgg cgcatttgct cgtcggcacc gggccgaagc tggccgatct gctcgatcag
      421 gggatggccg acatagctgc agggcggacc gcgcagcctg cggtaggcct cgggttcgaa
      481 aggcagcaaa ccaagcacat gatcgacata gcgccgcatc gcgcgcgccc ggccgggccg
      541 ccatgcccag accgatggcg agacataatc gataatcggt atcgagggat cgcgggcgcg
      601 gacgcgtctt gccacccgat gggtaaagtc ggggctgtcg atgatcacga gaatgtcggg
      661 cgaagcttcc gtcaccgcgg tggcggtttc ccggatgagc cgcagaattt tcggcaactg
      721 cttcacaacc gcggccaatc ccatgatcga cagctcttcg atcggaaaca gcgacaccag
      781 tccttcgcgc gccatcgatt ggccgccgac gccttcaaac cggacggcgc cgccaagccg
      841 ctggcgcagc accttcatca ggctggagcc aagccgatcc cccgattcct ccgtcgcgat
      901 cagaaatatt tttcgcgtga cgctgccgct tgttcgcgcc ggcatcacgc ggacaaaccg
      961 gtcacgaaaa ggcccgcggc atcggctgct tcgatcatgg tctgcggttc aacgacaatc
     1021 gtgttgccag caacgaccgc caccccggca agctctgccc tgcccgcgcc ttcgatcgtg
     1081 cgcggcccta tggtgggaag atcgaaacgc aaatcctgcg cgcttttcgg cgctttcacg
     1141 agcacaccgc gcgcagcctt ggcgcggatg cgaccttccg cgcgcagccg cgccacgcgc
     1201 gccagcaagc cgtcggtgcc ttcgatgtcc tccaccccga ccacgtggcc gtcgaccaca
     1261 atggcggctt gaccgatgtc gaatggactg agcgcccgca gcacgtcacg ccccctggcg
     1321 atatcggcag ccgcgctctg gtccggcgtc acctgcgtga tgcatccttc gggcatcagc
     1381 agatccggcg cgacatccct gattcccacc atccggaagc cgtcctgttc gagaatccgg
     1441 ccgatcccgg acagcagatg atcgtcgccg ccccggaaag ccgcccacac ccggccgagg
     1501 acgcgaagcg tgccccaatc cagcctgatc tcggataaag ccggccgcac cagggtgccg
     1561 atgaacacca gatcgcggca gttctcgctg cgaaacagtt ttaccgcccg cccgacctga
     1621 ccgaccgaga tccagtgatg gcgaaagcgc gccacccgga ccggatcgca ggcgcctttc
     1681 agcgcgaaaa caaccggatc gaggccgcgc gcgatcaggg aatccgcgac cgcgaacggc
     1741 atgacgccgc cagccgcaat caagccaatg ggcgatgaaa tctgcataac ctcgttcgtc
     1801 atggttcgcc ccgacgactc aagacctctt gccgtccttc accggctggc acaactcccg
     1861 atgcttggcg gcatcgatga aggtcaggat ttccgcaatc gcagggtctg cagccgcgag
     1921 atgcctcacc tcattgaggc ggtcggcaaa gacgccggac ccgtgaaaca gttgttgata
     1981 gaacaatctc acgaccgcga ggcgctcgcg ggtgaatttc cggcgtttca ggccgacggt
     2041 attgaggccc gttagcgccg cgaactggcc actcgccagc gtgaacggga tcacgtcgct
     2101 gcgcaccccg gtgagggcgc cgaccatcac ctgcgccccc acccgggtga actggtgcac
     2161 ggccgaaagg ccgccgatga aaacaaaatc gccgatctcg gcgtggccgc cgagcgtcgc
     2221 cgaggtcgcg aaaatcacgt cgttcccgac gatgcagtcg tgaccgacat ggctgcaatt
     2281 catgaaatag ccgcgctcgc cgacccgggt aatgccgccg ccagccatgg tgccggcgtt
     2341 catggtgacc tgctcccgga tggtgcaacc ctggccgatc gcgagcccgg tgagttcgcc
     2401 gcgatagctc agcgattgcg gcggcgtgcc gagcgaaacg aaggggtaga tggtgcagcc
     2461 gtcaccgatc gtggtctgcg ccgtaatatg cacatgcgcg atcagcttgc agttcgcgcc
     2521 gatcaccacg tgggggccga cgacgcagta ggggccgatc gacgtgccct ccccgatcac
     2581 agcgccatcc tccacccgcg cggtgggatc gatcgtactc atcaagtggg tccagtcagc
     2641 aaatctgttg ggttttccgg tggttaccgc gacataccgg cgctgtctag tgacgtatgg
     2701 ttacgccgta tttcggcatg ttgggcccac gtcaccctca tcagtccgtc agcatcgcgc
     2761 caacgtcggc ctcggcaacc gtcacaccgt taacggtggc gttgccgtga aaccaccaca
     2821 tggtcttgcg gcggccgatg ctgcgcatgt ggtattcgac ggtatcgccg ggcaacaccg
     2881 gcttgcggaa cttgcacttg tcgatggtga gaaaatagac cgcgcgcggc ttttcggtac
     2941 cttcgaccga gaggatgccg atgacgcccg cggtctgtgc catggcttcg atcatcatca
     3001 caccgggata gaccgggcgc tccggaaaat gcccttgaaa tgcgggctca ttgaaggtga
     3061 cgttcttgat accgatgccg ctgtagtcgc tgcggatgtt gatgacgcga tcgatcagca
     3121 gcatcggata gcgatgcgga agtgtcttga ggatggcatt gatgtccacc agctcaaatc
     3181 tgaccggtgc gtccgtcact cgcgcccctc gcccctatcg gccgtgccat cacggaccag
     3241 acgctccacc gcaatgatct cgcggaacca ttgcttggtc ggcttggcaa aatagccacc
     3301 ccagcgtccg ttggccggga tgtcatcctt gaccgcgctc atcgcggtga cctgagcgcc
     3361 gtcgccgata tgaagatggt tgttgattcc caccttggct cccagcgcca cattgtcgcc
     3421 aatcgtcagg ctgcccgcca gacctatctg ggccgcaagc agacaatgtc tgccgattgt
     3481 cacattgtgg ccgatctgga cctgattgtc gattttggtg ccctcgccga tcacggtgtc
     3541 acgcaggctg ccgcgatcga tggttgtacc ggcgccgatc tccacatcgt tctggatcag
     3601 gacgcggccg gtctgcggca ctttcagatg gccatccggt ccgaagaaga cgaagccata
     3661 gccgtcctga ccgatgctgc agccgggatg gatcagaacg ttgttgccga tcagcgcgca
     3721 ttgaatcgcc gtatgcgagc cgacgttaca gtccctgccg atcctgacat tgacaccgat
     3781 cacggcaccc gaaccgatga tcgtcccagc gccgatttcg acgtttggac cgatcacggc
     3841 aagcggatcg acaatcaccc catcttccag atgcgccgtc ggatcgatga cggcggatgg
     3901 agcgatgccg gcattgccga accaggattg cgggcgaagc gcgtcgccat gcagcacgcg
     3961 ggcaatcttc acaaagtcac ggaaagggtg ggccgcgcgc aaaaccgcaa catg
//
LOCUS       DASWGM010000586         8426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_324598,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000586 DASWGM010000000
VERSION     DASWGM010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324598"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VGA15_16685"
     CDS             <1..452
                     /locus_tag="VGA15_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX9589368.1"
                     /translation="MTRMQIERLYLLRRDLLRLRNAVGPLVEVCRRLEHDNLPMVRPA
                     MQPLFRDVTDHVRTVQEQIDSLREVLAFAFEASLLVGQAQETAVSKKLASWLAIIAVP
                     TAIAGIYGMNFKNMPELQWEYGYVTVMSVILITCAALYWRFRRVGWL"
     gene            complement(462..902)
                     /locus_tag="VGA15_16690"
     CDS             complement(462..902)
                     /locus_tag="VGA15_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9589369.1"
                     /translation="MKISDLLSPTDVMIDVRASNKRLLLQEFAAKAADSLDLPVDQIA
                     PYLLKREELGSTGIGRGVAIPHARLPDLQRPFGLLAKLRQPIEFDAIDGQPVDIVFTL
                     LMSAAAEDGQLGALALVARTLRPPENLARLRGAKNSSELYSAIV"
     gene            complement(1020..1709)
                     /locus_tag="VGA15_16695"
     CDS             complement(1020..1709)
                     /locus_tag="VGA15_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9589370.1"
                     /translation="MSAAPIKVLVIDDEPPIRKLLRMGLSTQGYEVLEASNGKTALEL
                     LVQNPALIILDLGLPDIQGHELLRMIRGRNDSVPIVVLSSRGDEAGKVQALDLGADDY
                     LTKPFGMDELLARMRAALRHQLQVHGERPVFRSGDLSVDLVRRIVKVGDKDVKLSPKE
                     YELLRVLVQHAGKVLTHRFLLKELWDELTDAQYLRVYVRQLRQKIEADSERPQFVLTE
                     TGIGYRLRAPD"
     gene            complement(1706..4429)
                     /locus_tag="VGA15_16700"
     CDS             complement(1706..4429)
                     /locus_tag="VGA15_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEX9589371.1"
                     /translation="MANQRRYLEKRPSPDALLEAARRESDHAGRLKIFVGAAPGVGKT
                     YEMLQSAHAKVKAGVDVVVGVVETHGRAETEALLQGLEVLPRKRIVYKDQSIEEMDLD
                     ALILRRPRIALVDELAHTNAPGSRHPKRYLDVEELLSHGIDVYTAVNIQHIESLNDVV
                     AQITHVRVRETVPDSVFDRADAIELIDLTPDDLIQRLKEGKVYVPKQAERALEHYFSP
                     GNLTALRELALRRTAERVDEQLLTHMQANAIAGPWAAGERILVCLSEDPRAAGLVRYT
                     KRLADRLHAPWTAISIETRRSLQLTDEQRDRLADTLRLAEALGGEAITIPGVGRRIAD
                     DVINFAQANNVTQIIIGKSTRSWWFETRRGSVVHDLVRRAGNISVNVIAGEELAAEPV
                     PRKTVRTAERTEPFDPKPSLMALLIVAIGLGAGELIQPIFGIENVDLVFLTAVVVVAV
                     RYGLWPSLLASVAASLCYNFFFLPPVYTFTITDPTNIAAFFFFMLIAILVSNVAARVR
                     TQADSAIGRVRTTESLYAFSRKLAGTATLDDVLWATAYQTALMLKVRVVLLLPEDGIL
                     TVKAGYPPEDELDKADLAAANWAWGNDRSAGRGSDTLPGAKRLFLPMRTGRGAIGVVG
                     IDDDKTGPLLTPDQRRLLDALMDQGALAIERVLLVEDMDRVKRTVESDRLRSALLTSI
                     SHDLKTPLASVLGAASTMRDLSSGLTDTEKRDLLATVIDESERLNRFIANLLDMTKLE
                     SGAIVPNTAPYDIGEIVGSVLRRASKILGNHQVSLELAADLPMLELDAVLFEQVLFNL
                     LDNAAKYAPADTTISIRGLQDRDWVALQIIDEGDGIPPAELESIFDKFYRTQKGDHVR
                     PGTGLGLAISRGFVEAMHGTISATNRIDRKGAVLTIRLPIPAASEALDTAA"
     gene            complement(4471..5076)
                     /locus_tag="VGA15_16705"
     CDS             complement(4471..5076)
                     /locus_tag="VGA15_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HEX9589372.1"
                     /translation="MLKEIRPAIILLVLLTAITGLAYPLAMTAIAGVIFPKQAQGSLI
                     ERDGKVVGSALIGQEFKDDKYFHGRPSATTAPDPADATKTVPAPYNAANSGGSNLGPT
                     SKALNDRVKEDVDKLKAGNPSMPVPIDLVTTSGSGLDPDISPEGALFQVPRVAKARNM
                     PEDRVRELVTANTQGRLAGLLGEPRVNVLALNLALDAAAAK"
     gene            complement(5183..7297)
                     /gene="kdpB"
                     /locus_tag="VGA15_16710"
     CDS             complement(5183..7297)
                     /gene="kdpB"
                     /locus_tag="VGA15_16710"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX9589373.1"
                     /translation="MEAMKLQKRMPVSAMLDPKIVIPAIGSAFAKLDPRLMIKNPVMF
                     VVEIVAALTTMIFLKNVVSGGESLAFTFQIILWLWFTVLFANFAEAVAEGRGKAQAES
                     LRKTRTESQAKLLTGSDNAYRLVSGTSLKVGDIVLVEAGDNIPSDGEVIEGVASVNEA
                     AITGESAPVIRESGGDRSAVTGGTQVLSDWIRVRITAAQGSTFIDRMIKLVEGAERQK
                     TPNEIALNILLAGLTIIFVFATVTIPSYAAYAGGQISVVILVALFVTLIPTTIGALLS
                     AIGIAGMDRLVRFNVLAMSGRAVEAAGDVDTLLLDKTGTITLGNRQATAFRPVRGVTE
                     QELADAAQLASLADETPEGRSIVVLAKEKYGIRSRDMAELAATFIPFTAQTRMSGVDA
                     GASSVRKGAVDAILNYIDGGGHSRVVASGNAARILHPAMSEASRELQAIADEIAKAGG
                     TPLAVAKDGRLLGIVQLKDIVKGGIRERFAELRRMGIRTVMITGDNPMTAAAIAAEAG
                     VDDFLAQATPEDKLKLIRDEQAKGKLVAMCGDGTNDAPALAQADVGVAMNTGTQAARE
                     AGNMVDLDSNPTKLIEVVEIGKQLLMTRGALTTFSIANDVAKYFAIIPAMFLAFYPQL
                     AVLNVMHLASPQSAILSAIIFNALIIIALIPLALKGVAYRAIGAGALLRRNLMIYGVG
                     GIIIPFIGIKAIDLAVAALHLA"
     gene            complement(7323..>8426)
                     /gene="kdpA"
                     /locus_tag="VGA15_16715"
     CDS             complement(7323..>8426)
                     /gene="kdpA"
                     /locus_tag="VGA15_16715"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX9589374.1"
                     /translation="TLGAYVDATTLEGAKQTIAVGPVASQVAIKMLGTNGGGFFNANA
                     AHPFENPTALSNFVQMISIFALGAALTNVFGRMVGNQRQGWAILAVMGVLFISGVIVT
                     YWAEANGTTALQALGLTGGNMEGKEVRFGIVASSLFAVITTAASCGAVNAMHDSFTAL
                     GGMIPLINIQLGEIIVGGVGAGLYGMLLFVVLSIFVAGLMVGRTPEYVGKKIEAREVK
                     MAMLAILVLPLMYLGWTAVAVVYPTAVASMANAGPHGFTEVLYAYTSQTGNNGSAFAG
                     LTGNIPFYNITGGVAMFVGRFFMIVPAMAMAGSLAGKKSIPPSAGTLPTTGGLFVGLV
                     VGVILIIGGLTFFPALALGPIVEHLAGNANTLF"
BASE COUNT         1574 a         2614 c         2678 g         1560 t
ORIGIN      
        1 cgatgacgcg gatgcaaatc gagcggcttt atctgctgcg acgcgacctg ttgcgcctgc
       61 gaaacgcggt cgggcccctg gtggaggtct gccgcaggct tgagcatgac aatctgccga
      121 tggtgcggcc tgccatgcag ccgctgttcc gcgacgtcac agaccacgtc agaacagttc
      181 aggagcagat cgactcgctt cgagaggtcc tcgcatttgc cttcgaggca agtcttctgg
      241 tcggtcaggc tcaggagacc gcggtctcca agaaactggc ctcatggctg gccatcatcg
      301 cggtcccgac tgcgatcgcc ggaatctacg gcatgaactt caagaacatg ccggaactgc
      361 aatgggaata tggttacgtc accgttatga gcgtgatcct gattacgtgc gcggcgctgt
      421 actggcgatt ccggcgcgtc ggatggctgt gagtccgatc gtcagacgat cgccgaataa
      481 agctcggatg aattctttgc gccgcgcaat cgcgcgagat tctccggcgg tcttagcgtc
      541 cgggctacca gcgcgagcgc gccgagctgt ccgtcctccg cagcggccga catcaagagg
      601 gtaaatacga tgtccaccgg ctgcccgtcg atggcgtcga attcaatcgg ctgccttagt
      661 ttcgcaagca gcccgaaggg ccgttgcaaa tccggcagcc gggcatgcgg aatggctacg
      721 ccacggccta tcccggtcga tccgagttct tccctcttga gaagataggg cgcgatctga
      781 tcgacaggca ggtcgagact atcagcggct ttggccgcga attcctgcaa cagcagccgc
      841 ttgttggagg ctcgaacgtc gatcatgacg tccgttggcg acagcagatc ggagattttc
      901 atggtcgctc caggaaggcg catcgttaaa agcattgatt aggtctcagt taggagggcg
      961 gtttacgcca tcctcccgac aacgggaaga gatcaaaagc taaagccaac tgccgcccat
     1021 taatccggcg cgcgcagccg atagccgatt ccggtttcgg tcaagacgaa ttgcggacgc
     1081 tccgaatcgg cttcgatctt ctgccggagc tggcggacat agacccgcag atattgcgcg
     1141 tcggtcagct cgtcccatag ctccttcagc aagaacctgt gcgtcaacac cttgccggcg
     1201 tgctgcacca gcacgcgcag caattcgtac tccttcggcg acagcttgac gtccttgtcg
     1261 ccgaccttga cgatacggcg caccagatcg acgcttagat cgcccgaccg aaacaccgga
     1321 cgctcgccat ggacctgcaa ttggtgccgc aaagccgcgc gcatgcgggc gagcagttcg
     1381 tccatcccga acggctttgt cagataatcg tcggcaccga gatcgagcgc ctgaactttt
     1441 ccggcctcat caccgcggct cgacagcacc acgatcggaa cgctgtcgtt gcgcccgcgg
     1501 atcatgcgca gcaattcatg gccctggata tcgggaaggc cgagatcgag aatgatgagg
     1561 gccggattct ggaccagcaa ttcgagcgcg gtcttgccgt tggaggcttc caggacctcg
     1621 tagccctggg tgctcaatcc catccgcagc aatttgcgga tcggcggctc atcgtcgatg
     1681 acaaggacct tgatcggcgc ggcgctcatg cggcggtgtc cagcgcttct gatgccgccg
     1741 ggatcggcag tctgatggtc agcacggcgc cctttcgatc gatgcggttg gtggccgaaa
     1801 tcgtgccatg catcgcctcg acaaagccac gggatatcgc gagcccgaga ccggtgccgg
     1861 ggcggacatg gtcgcccttt tgcgtgcgat agaatttgtc aaatatgctt tccagctccg
     1921 ccggtggaat gccgtcgcct tcgtcgatga tttgcagggc gacccaatcc ctgtcctgca
     1981 aacccctgat cgatatcgtg gtgtcggcgg gtgcgtattt cgccgcgttg tccaggagat
     2041 taaaaagcac ctgttcgaac aataccgcgt cgagctccag catcggcaag tcagccgcaa
     2101 gctcaagcga gacctggtga ttgccgagga tcttgctggc gcgccgcaac acactgccga
     2161 cgatctcgcc gatatcgtac ggcgcggtat tcggcacgat ggcgcctgat tccagcttgg
     2221 tcatgtcgag cagattggcg atgaaccggt tgaggcgttc ggactcgtcg atcacggtag
     2281 cgagcagatc gcgcttttcg gtatcggtca ggccgctcga aagatcacgc atcgtagagg
     2341 cggcgccgag aaccgaggcc agcggcgtct tgagatcgtg cgagatcgag gtcagcagcg
     2401 ccgagcgcaa ccggtccgac tccacggtgc gcttgacgcg gtccatgtct tcgaccagca
     2461 gcacccgttc gatcgcaagc gcgccctggt ccatcagcgc atcgagcaga cgacgctggt
     2521 ccggcgtcag cagcggtccg gtcttgtcgt catcgatacc gacgacgccg atcgcgccgc
     2581 gtccggtccg catcgggagg aacaggcgct tggcgcccgg cagcgtgtcc gaccctcgcc
     2641 cggccgagcg gtcgttgccc caggcccagt tcgcggcggc gagatcggcc ttgtccagtt
     2701 catcttcggg cggatagccc gcctttaccg tgaggatgcc gtcctccggc agcagcaata
     2761 cgacccgtac cttcagcatc agcgccgttt gataggccgt cgcccacaac acatcgtcca
     2821 acgtcgcagt acccgcaagc ttgcggctga aggcgtaaag cgattccgtg gttcgcaccc
     2881 tgccgatcgc ggagtcggcc tgggtgcgca cgcgtgccgc gacattggag accaggatcg
     2941 cgatcagcat gaagaagaag aacgccgcga tattggtcgg gtcggtgatc gtgaaggtat
     3001 agaccggcgg caaaaagaag aaattgtagc acagcgacgc cgcgacgctg gcgagcaacg
     3061 acggccacag gccgtaacga accgccacga ccaccactgc cgtgaggaat accagatcga
     3121 cattctcgat gccgaatatt ggctggatca actcgccggc gccgaggccg atggccacga
     3181 tcaggagcgc catcaaagac ggcttgggat cgaatggctc ggttcgctcc gccgtgcgta
     3241 cggtcttcct tgggaccggc tcagctgcga gttcctcgcc ggcaatcacg ttgacgctga
     3301 tattgccggc ccgccgcacc aggtcgtgca ccaccgagcc gcgcctggtt tcaaaccacc
     3361 aggaacgcgt cgacttccca atgatgatct gggtcacgtt gttggcttgc gcgaagttga
     3421 tgacatcgtc ggcgatgcgg cgcccgacgc cgggaatggt gatcgcctca cctcccagcg
     3481 cttccgccag acgcagcgtg tcggcaagcc ggtcgcgctg ctcgtcggtc aattgcagac
     3541 tccgccgcgt ctcgatgctg atggcggtcc agggcgcgtg cagccggtcc gccagccgct
     3601 tggtgtaacg caccagcccc gccgcacgcg gatcctcgct aaggcagacc aggatgcgtt
     3661 cgcccgcggc ccaggggccg gcaatggcat tggcctgcat gtgagtcagg agttgctcgt
     3721 cgacgcgctc ggcggtgcgc cgcaacgcca gctcgcgcaa tgccgtcaga ttgcccggcg
     3781 agaaataatg ctcgagcgcg cgttcggcct gctttgggac atagaccttg ccctctttca
     3841 gccgctggat cagatcgtcc ggcgtgagat cgatcagttc gatggcgtcc gcgcggtcga
     3901 acacggagtc cggcacggtc tcgcgcaccc gcacatgggt gatctgggcg accacgtcgt
     3961 tgaggctttc gatgtgctgg atattgacgg cggtgtagac gtcgatgccg tgcgagagta
     4021 actcctcgac gtcgagatag cgcttcggat ggcggctgcc cggcgcgttg gtatgagcga
     4081 gttcatcgac cagcgcgatc cgcggacggc gcaagatcag ggcgtcgaga tccatctcct
     4141 cgatcgactg atccttgtac acgatccgtt tgcgcgggag aacttcgagg ccttgcagca
     4201 gcgcctccgt ctccgcccgt ccgtgggtct cgaccacgcc aacgacgaca tcgacgccag
     4261 ccttcacttt ggcatgcgcg ctctgcagca tctcgtaggt cttgccaacg ccgggggcgg
     4321 cgccaacgaa gatcttgagc cgcccggcat gatcgctctc tcgccgggcg gcctcgagaa
     4381 gcgcgtccgg cgaaggtctt ttttcaaggt agcggcgttg atttgccata cggcaatata
     4441 gtcctcacgc ccgatgccgc ctaggccggg ctatttggct gcagccgcgt ccagcgccag
     4501 attgagcgcc agcacattga cccgcggttc gccgagcaat ccggcaaggc ggccttgcgt
     4561 attcgccgtg accagctcgc ggacccggtc ttccggcata ttccgcgcct tggcgacccg
     4621 cggcacctgg aatagcgcgc cctccggcga gatgtcggga tcgaggccgc tgccggacgt
     4681 cgtgacgaga tcgatcggaa cgggcatgga tggatttccg gctttcaact tgtcgacgtc
     4741 ttctttgacc cgatcgttca aggccttgct ggtcgggccg aggttggatc cgcccgaatt
     4801 ggcggcattg tagggcgctg ggacggtctt ggtggcatcg gccggatccg gcgctgtcgt
     4861 ggccgagggc cggccatgga agtacttgtc gtccttgaat tcctgcccga tcagggccga
     4921 gccgaccacc ttgccatctc tttcgatcag gctgccctgc gcctgcttcg ggaagatcac
     4981 gcctgcgatg gcggtcatgg cgaggggata ggcgaggccg gtgatcgccg tcagcaggac
     5041 gagcaggatg atggcggggc gaatttcttt taacatgata agtctccaga cttttcgtca
     5101 ttgcgagcga agcgaagcaa tccatggaac gaatgaaaga ctggattgct tcgtcgcttc
     5161 gctcctcgca atgacggagt gattaagcta agtgcaaggc ggcgacggcg aggtcgatgg
     5221 ccttgatgcc gatgaacgga atgatgatgc cgccgacgcc atagatcatc aggttgcggc
     5281 gcagcagcgc gccggccccg atggcccgat acgccacgcc tttcagggcc agcggaatca
     5341 acgcgatgat gatcagcgca ttgaagatga ttgccgacag gatggcgctc tgcgggctcg
     5401 ccagatgcat gacgttgagc accgcaagct gcggatagaa cgccaggaac atcgccggga
     5461 taatcgcaaa atattttgcg acgtcgttgg cgatcgagaa cgtggtcagc gcgccgcggg
     5521 tcatcagcaa ttgcttgccg atctcgacca cctcgatcag ttttgtcgga ttggaatcga
     5581 gatcgaccat gttgccggcc tcgcgcgccg cttgcgttcc ggtgttcatg gcgacgccga
     5641 catcggcctg ggcgagtgcc ggcgcatcgt tggtgccgtc gccgcacatc gccaccagct
     5701 tgcccttggc ttgctcgtcg cgaatgagct ttagcttgtc ctcaggggtc gcttgcgcga
     5761 ggaaatcgtc gacgccggct tccgccgcga tcgccgccgc ggtcatcgga ttgtcgccgg
     5821 tgatcatcac ggtgcggatg cccatgcggc gcagttcggc gaagcgctcg cgaatgccgc
     5881 ccttgacgat gtccttgagc tggacgatgc caagcaggcg gccgtccttg gcgaccgcga
     5941 gcggggtgcc gccggccttc gcgatttcgt ccgcaatcgc ctgaagctcg cgggaagcct
     6001 cggacatggc ggggtgcaga atgcgcgcgg catttcccga cgccacgacg cgggagtggc
     6061 cgccgccgtc gatataattc agaatggcat cgaccgcgcc cttgcggact gacgatgcac
     6121 ccgcatcgac gccgctcatg cgggtttgcg ccgtgaacgg aatgaaggta gcggcaagct
     6181 ccgccatgtc acggctgcgg atgccgtatt tttccttcgc cagcacgacg atcgaacggc
     6241 cctcgggggt ttcatcggcg agcgaagcaa gctgcgccgc atcggcaagt tcctgctcgg
     6301 tcacgccgcg gacggggcga aacgccgtcg cctggcggtt gccgagcgtg atggtgccgg
     6361 tcttgtcgag cagcaaggta tcgacgtcgc ccgcggcctc gaccgcgcgg cccgacatcg
     6421 ccagcacgtt gaaacgcacc agccggtcca tgccggcgat gccgatcgcc gatagcagcg
     6481 cgccgatggt ggtcgggatc agcgtcacga acagcgccac caggatcacg actgatatct
     6541 ggccaccggc atacgccgca tagctcggga tggtcaccgt cgcgaacacg aagatgatgg
     6601 tcaggcccgc gagcaggatg ttgagcgcga tctcgttcgg cgtcttctgc cgctcagcgc
     6661 cctcgaccag cttgatcatg cggtcgatga aggtcgagcc ctgcgccgcg gtgatgcgga
     6721 cgcggatcca gtcggacagc acctgggtgc caccggtgac cgccgagcga tcgccgccgg
     6781 attcgcggat cacgggcgcg gactcgccgg tgatcgcggc ctcgttgacg gaggcgacgc
     6841 cttcgatcac ttcgccgtcg gacggaatgt tgtcgccggc ctcgaccagg acgatatcgc
     6901 cgactttgag gctggtaccg gacaccagcc gataggcgtt gtcggagccc gtcagcagct
     6961 tggcctggct ctcggtgcgg gtcttcctga gcgactccgc ctgcgccttg ccgcggcctt
     7021 ccgcgacggc ctcggcgaag ttggcgaaca gcaccgtgaa ccaaagccac aagatgatct
     7081 ggaaggtgaa agccaggctc tcgccgcccg agacgacgtt cttgaggaag atcatcgtgg
     7141 tcagcgcagc cacgatttcg accacgaaca tcacggggtt cttgatcatc agccgcggat
     7201 cgagttttgc aaacgccgag ccgatcgccg ggatcacgat cttcgggtcg agcatcgccg
     7261 ataccggcat gcgcttctgc agtttcatgg cttccatgga agtaactccg aaatgtggtc
     7321 gatcagaaca gggtgttggc gttgccagcg agatgctcga cgatcggacc gagcgcgagc
     7381 gccgggaaga aggtgagacc gccaatgatc aggatgacgc cgaccaccag gccgacgaac
     7441 aggccgcccg tcgtcggcaa cgtgccggcc gacggcggga tcgacttctt gcccgccagc
     7501 gaacccgcca ttgccatggc cggcacgatc atgaagaagc ggccgacgaa catcgccacc
     7561 ccaccggtga tgttgtagaa tgggatgttg cccgtaagac cggcgaaagc cgagccgtta
     7621 ttgccggttt gcgaggtgta ggcatagagc acctcggtaa agccgtgcgg tcccgcattg
     7681 gccattgagg caacggcggt cggataaaca acggcgacag cggtccagcc gagatacatc
     7741 agcggcaata cgaggatcgc gagcatcgcc atcttgactt cgcgcgcctc gatcttcttg
     7801 ccgacatatt ccggcgtgcg gccgaccatc aagccggcga cgaagatcga gagcacgacg
     7861 aacaacagca tgccgtagag gccggcgccg acgccgccga cgatgatttc accgagttga
     7921 atgttgatca gcggaatcat gccgccaagc gcggtgaacg agtcatgcat ggcattgacg
     7981 gcaccgcagg acgccgccgt ggtgatgacc gcgaacagcg acgaggcgac aatgccgaag
     8041 cggacttcct tgccttccat attgccgccg gtcagcccca gcgcctggag cgcggtggtg
     8101 ccgttggcct ccgcccaata ggtgacgatg acgcctgaga tgaacagcac acccatcacg
     8161 gccaggatgg cccagccttg ccgctggttg ccgaccatgc ggccgaacac gttggtgagt
     8221 gcggcaccga gcgcgaagat cgagatcatc tgcacgaaat tagacaacgc ggtcgggttc
     8281 tcgaacggat gcgcggcatt ggcattgaag aagccgccgc cattggtgcc gagcatcttg
     8341 atcgcgacct gggaggccac agggccgacc gcgatggtct gcttggcgcc ttccagcgtc
     8401 gtggcatcga cataggcgcc gagcgt
//
LOCUS       DASWGM010000587         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2288834,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000587 DASWGM010000000
VERSION     DASWGM010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288834"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VGA15_16720"
     CDS             <1..204
                     /locus_tag="VGA15_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 domain-containing protein"
                     /protein_id="HEX9589375.1"
                     /translation="GEGHIAYVKQIRSEDVPGLFPQAPKIAPGLKLFALHAADGTPIM
                     LTDSREAAIANAWSNELQAVSVH"
     gene            complement(465..926)
                     /gene="ptsN"
                     /locus_tag="VGA15_16725"
     CDS             complement(465..926)
                     /gene="ptsN"
                     /locus_tag="VGA15_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEX9589376.1"
                     /translation="MTITDLVAPEAILPALKVNSKKQALQELAARAASLTGQNERSVF
                     EVLLQREKLGTTAVGYGVAIPHGKLPKLEKLFGLFARLERPIDFEAMDGQPVDLVFLL
                     LAPEGAGADHLKALARIARLLRDQDVAKKLRASRDAQAIYSVLALPPASAA"
     gene            complement(1144..1755)
                     /gene="raiA"
                     /locus_tag="VGA15_16730"
     CDS             complement(1144..1755)
                     /gene="raiA"
                     /locus_tag="VGA15_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX9589377.1"
                     /translation="MTLRISGKSISVGDALRGRVSERTDEVLRKYFDGNYSGHITLSK
                     DGFGFRTDCALHLDSGITLEADSNAVDAYSSADQALAQIEKRLRRYKSRLKDRSARKS
                     HAEAVAMVELTAPTLKAPSYVIEAPAGDDEHDDTGYSPVIIAEATTSLKRMSVSEAVM
                     ELDLTGAACLVFQHGSNGRVNVIYRRPDGNVGWVDPPVVNSGG"
     gene            complement(1812..>2698)
                     /gene="rpoN"
                     /locus_tag="VGA15_16735"
     CDS             complement(1812..>2698)
                     /gene="rpoN"
                     /locus_tag="VGA15_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX9589378.1"
                     /translation="NRYDPAMQALVEHLDLLAKRDIAALRKFCGVEDEDITDMIGEIR
                     RLDPKPGLKFGSARTQTMVPDVYVRPGPDGGWHVELNSDTLPRVLVNQVYYTELSKTI
                     RKDGDKSYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFTQGVAHLRPLNLK
                     AVADAIQMHESTVSRVTANKYMATNRGSFELKYFFTASIASADGGEAHSAEAVRHHIK
                     QLIDAEDPAVILSDDTIVERLRASGIDIARRTVAKYREAMRIPSSVQRRRDKQSMLGN
                     ALSAPAASSDRSRDPAPV"
BASE COUNT          499 a          839 c          835 g          525 t
ORIGIN      
        1 ggcgagggcc atatcgccta tgtgaaacag atccgttccg aggacgtgcc ggggctgttt
       61 ccccaggcgc cgaagatcgc gccgggactg aagcttttcg cactccatgc cgccgacggc
      121 acgccgatca tgctgaccga cagccgggaa gcggcgatcg cgaatgcatg gagcaacgag
      181 ctgcaggccg tcagcgttca ctgacggagc tcttgctcct gtcactgacc cggcacttgc
      241 cggtgtcact gacgcaggcc ttgccggtgt caatggcgcg gggccttgcg gcgtcactgc
      301 cgccgtgtga tctggatgat gatgtcgtac gcgggcttga cccgcgtatc catcgatctt
      361 tctaggaaga attttcaaaa gaagatggat tgccgggagc gcagacaagt tcacgtagtc
      421 tgcgcaaggc agactactat gcccggcaac gacggctgtg gcatctaggc agcactcgcc
      481 ggcggcaggg ccagtaccga ataaattgcc tgcgcatcgc gcgaggcgcg caatttcttg
      541 gcaacgtcct gatcgcgcaa cagccgtgcg atgcgcgcca gcgccttcag gtgatcggcg
      601 cccgcgccct ccggcgccag caacaggaac accaggtcga ccggctggcc gtccatcgcc
      661 tcaaaatcga ttgggcgctc aagacgggca aaaagcccga acaatttctc cagtttgggc
      721 agcttgccgt ggggaatggc gacgccatag ccgacggcgg tggttcccag cttctcgcgc
      781 tgcagcaaca cctcgaaaac cgagcgttcg ttctgcccgg tcaggctagc tgcccgcgcc
      841 gccagttcct gcagggcctg tttcttgctg ttgaccttca acgccgggag aatcgcctcg
      901 ggtgcgacca gatcggtaat cgtcatgggg cgttccgagg tgaatttaac cgtcaggtta
      961 cgaatgggct tcagaggggg cggcgtcggg aagatgaaac aggtttacac ccgggtccca
     1021 tttggtgggc ttctactcca acgcaagacc ggtgccaact cctgcgaagc tgtttccggt
     1081 cattaccccc aagggcggcg gaccatagga aggggtcctg ggcgcgtcaa tgccgattgg
     1141 cgcctatccc ccggagttga ccacgggggg atcgacccag ccgacattgc cgtccggccg
     1201 ccggtaaatg acgttgaccc gcccgtttga gccgtgctgg aacacgaggc aggcggcacc
     1261 cgtcaggtcc agttccatca cggcctcgct caccgacatc cgctttagcg acgtcgtcgc
     1321 ctcggcgatg atcaccgggc tgtagccggt gtcgtcgtgc tcgtcgtctc ccgcgggcgc
     1381 ctcgatcacg tagctcgggg ctttgagggt tggcgcagtc aattcaacca tcgcaaccgc
     1441 ctcggcatgc gactttcgcg cggaacgatc cttgaggcgg ctcttgtagc ggcgcagtcg
     1501 cttctcaatc tgagccagcg cctggtcggc gctggagtag gcgtcgacgg cattcgaatc
     1561 ggcctcgagc gtaattccgg aatccagatg cagcgcgcag tcggtgcgga agccgaaacc
     1621 atccttgctc agcgtgatat ggccggaata attgccgtcg aaatatttgc gcaggacttc
     1681 atcggtgcgc tcgctgacgc gtccgcgcaa cgcgtcgccg acactgatgc tttttcccga
     1741 gatccggaga gtcatttcga tgcctcgatt cgtctgcagc gggacagtgt cccgaattgc
     1801 cggggacgca atcaaaccgg tgcggggtcg cgcgaccggt ccgacgaagc ggcaggcgcc
     1861 gacagggcgt tgccgagcat gctctgtttg tcgcggcggc gctgcaccga ggacggaatt
     1921 cgcatcgcct cgcggtactt agcaaccgtg cgacgggcaa tatcaatgcc cgaagcgcgc
     1981 aaccgttcca cgatggtgtc gtccgacagg atcaccgccg gatcttccgc atcgatcagc
     2041 tgcttgatgt gatgacggac ggcctccgcc gaatgcgctt cgccgccatc ggccgacgcg
     2101 atcgacgcgg tgaagaaata cttcaattcg aaactgcctc gattggtcgc catgtatttg
     2161 ttggccgtta cccgcgacac cgtcgattca tgcatctgga tcgcatccgc caccgccttg
     2221 aggttgagcg gccgcagatg cgccacgccc tgggtaaaga agccatcctg ctggcgcacg
     2281 atttcggttg cgaccttgag gatggtacgg gcgcgctgat cgagcgcgcg caccagccag
     2341 gtcgcgttct gcaggcaatc ggtgaaatag gatttgtcgc catccttgcg gatcgtcttc
     2401 gacaattccg tgtagtagac ctgattgacc agcaccctcg gcagcgtgtc gctgttgagt
     2461 tcgacgtgcc agccgccgtc ggggccgggc cgcacataga catccggcac catggtctgg
     2521 gtccgcgccg aaccgaactt caagcccggc ttcggatcga gacggcgaat ttcgccgatc
     2581 atgtcggtga tgtcttcatc ctcgacaccg cagaattttc gcaacgccgc gatatcgcgc
     2641 ttcgccagaa gatccagatg ctcgaccagc gcctgcatcg cagggtcgta acgattga
//
LOCUS       DASWGM010000588         9642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1288789,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000588 DASWGM010000000
VERSION     DASWGM010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9642
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288789"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VGA15_16740"
     CDS             <1..894
                     /locus_tag="VGA15_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9589379.1"
                     /translation="AKKFTAETGVPVEFITGGTIDRLNKANLAKGNPESDLTFTTSHV
                     GWIYATDGLFEELDTTKIPNAKNLADEARISPFHLGAWAYVYTIGYRADLLGGMKFES
                     WEDLWKPELKAKIAAPDFDPSHIIAISAILSGGDAAHWEKGQDKLKALKPNFKAFYTN
                     DANSQQLLASGETPVQVVLSMNAYYMISQGVPITLAIPKEGAVLGVDTVAIMKGSKKS
                     ELAYKFINALYDPEVQAEIAKIKKGSPAVLNAKIDPEIAKLPGVFTTAAQWKQQINID
                     AKLRAEKTAEWRKWFAENIMN"
     gene            917..1768
                     /locus_tag="VGA15_16745"
     CDS             917..1768
                     /locus_tag="VGA15_16745"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589380.1"
                     /translation="MNPQPFLRPFTAPAVLCTVGLLAAMAAVLQFSFRAYVPGSLEPG
                     GFTLANFADLLKPLYARVFLDTVWICFLTAISTLIVGYPLAYALVRTQNAILKSTLLV
                     IVVTPLFLGEVVRTYSWIIVLGNNGFINSVLIYLGIVEQPFQFMFTTFGVVVALVHVT
                     LPVMVIMLAAGLSHIDRDYQKAAESLGAGPVRTFLTITLPLSMPGVVAGVTTAFAWTF
                     SAFASPQLIGGGKVNMISNLVYQLGFASFNFPFAASLCVAGLVLTIAVLSMLRLAARP
                     LDNIRAH"
     gene            1770..2588
                     /locus_tag="VGA15_16750"
     CDS             1770..2588
                     /locus_tag="VGA15_16750"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589381.1"
                     /translation="MPRSTYQLGLGFVGKLAVLLILGFLVLPVAVVTIAAFNDRAILS
                     FPPQAWSLRWFAKAIAYQDFRDGFRNGLIVTAWSSTIALLVGAMFAFALDRYRFRFRK
                     LIEAILLSPLVIPHFTVGLGFLILAAQIGWTRGYAVVIFCHVVLVLPFVIRSVYVSLR
                     NLDQSYELAAASLGAPPLRVLLTVTLPLLLPGLVSGWLFAAILSFNEFTASLFVTAQR
                     TQTLPVAMYNYVREYADPSMAALSVMYIVGTAALIILANAFLGLGKVLNIEHTR"
     gene            2572..3711
                     /locus_tag="VGA15_16755"
     CDS             2572..3711
                     /locus_tag="VGA15_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589382.1"
                     /translation="MNILADTGVAAQTAGFAAGDDVAVRFDSVTKRFGEAVALREVSL
                     SVRRGEFMTLLGPSGCGKTTLLNLAAGFLSPDGGDIRIGGERVNDVPTYKREIGMMFQ
                     NYALFPHMSLADNIAYGLKVRHVPKKERDRRVEEVLALVRLTGLEGRKPRQLSGGQQQ
                     RVALARALVINPTVLLLDEPFSALDKNLRASMQVELREIQRKLEVTTIFVTHDQGEAL
                     SLSDRLAVMSEGRIRQLGTPEEIYRRPCDRFVASFVGDANVLRGRLDGLDGAHAVVAV
                     GAARVKVPASSLRGVTASAPVDLFVRPEQLQMVDSDAPCATSGTVAAQVYQGGHVDLY
                     IDCAESVRDRLLVRSARHDAMTRWPIGAQVGISIGDDDAVAFATG"
     gene            complement(3718..3906)
                     /locus_tag="VGA15_16760"
     CDS             complement(3718..3906)
                     /locus_tag="VGA15_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589383.1"
                     /translation="MAKTTEESKASMLEAFETLFNQRDYEAAKRYWSSNYIQHIAPGG
                     ATALRMRRPLAAASEAQR"
     gene            4404..5732
                     /locus_tag="VGA15_16765"
     CDS             4404..5732
                     /locus_tag="VGA15_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589384.1"
                     /translation="MRVLALASVGEKSSARAKTYVAGPKLIPRRHALMQVLLIASFAM
                     SGRAEAACTPASPVNNTIVTCTGKTTNQNGTNGYGTVNDNNNTYNIQAGATVTGDGFG
                     VVFGTGATINNDGTITAGIGVSGSDATINNTSTGVITGDTNGIQATTATVRNAGLISA
                     MGVSATGGNGFGISADTADVSNTSTGHITGTFTGIGTSTLAAVNNAGAISGGRLGITG
                     DTARVINSGAIAAGAAAIFASTANVTSTGAGTISGGTFGIVANTVASIANAGIILGNI
                     GIQASGAATITNSSAIISTAGASGTAIKLSNAADTLTLLTGSRIVGLLDMGFGNDTVN
                     TLTVAPGSKVSLPTTAAALPTLINFSGVTNTSFTGGRFTDPAVQAGNHLATLDPTALA
                     ADRPRLDRPHRQACRRWCRDASMAWRRPRTLRAHQGLRHPLALKGSKNDR"
     gene            complement(5820..6413)
                     /locus_tag="VGA15_16770"
     CDS             complement(5820..6413)
                     /locus_tag="VGA15_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589385.1"
                     /translation="MVIIPVDAHLLTLTISTPEIVEHPVRVGRETRMQRERIQRTREI
                     KTTRSAASLISSFKEVNKIFGAADIEFRLRYTTSDLVEAPKGSEDLDDEGFLMLAKDF
                     PMKNAVSLLLVRRFVGSEGGASKKELRVCAVGDDANDTSLAHEFGHLLWLDHQGDIRD
                     LMNPGLAVPGAPLTRKEIGDVRKSPLARQFGGRPSKG"
     gene            complement(6429..7415)
                     /locus_tag="VGA15_16775"
     CDS             complement(6429..7415)
                     /locus_tag="VGA15_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589386.1"
                     /translation="MRCELEEDDIGNFPGVTLFTKGTAHCIKPTAVYLPPKPAALGNM
                     LDVVIWLHGFAVRDHKFLFRNDPARIREQVRDSNKNVVLIAPFLGFEYFVKDKFAGNY
                     SAKDLAKPNWGERYLNEVLAAISHFQVPDLKSPPQLQIGKLVIACHSGGGNGMRNLVG
                     SLGKYQSNLKACWGFDCLYDGSGPPDHATFWYRWLSGPNARPLYIIYGPDTLPQSVKL
                     DLTGRGLATPEGNKAEPRRPALKNLNVSIGHYDAFPAFGQMVKVNDLDPSFVDRFMIP
                     PTADGAPSMHKSWPQGKFLGQAITNVREAFPFPKDIHYMIARGGFLDRLSKL"
     gene            complement(7514..8584)
                     /locus_tag="VGA15_16780"
     CDS             complement(7514..8584)
                     /locus_tag="VGA15_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589387.1"
                     /translation="MADGSQRTDYMAMEELKKRAQEHIARGDQPLGETRMLVVKFDTN
                     FDRFAANVAYELRHLVVYRTDGGNFARGLVRQTGQLANFHRELLENSIANGTRVSVAA
                     RLYRRANPDPTLRWMMSVQFGRPVIAVSQNEGGQIYASPAVAEQSGKNLAELVLQHAA
                     IVVENPHWSGDGQKRLRDWIKLAQQIGYPGFLDLWYYNRVAVFEYVNWDTTDGRRKAM
                     TTRTAGKLPFDGTTGSMSRGVSFSAAPDTPWRNYPFRNAIKKCVGQRNIDDCHGLIGN
                     ELRFAESEIFQSIQEMNKQIKRVSVVAGLDFFSVSGAPSDSLGPLAGMFYKDIRTRLL
                     DPKSLYATYMKYSQDTSGVPIW"
     gene            complement(8776..>9642)
                     /locus_tag="VGA15_16785"
     CDS             complement(8776..>9642)
                     /locus_tag="VGA15_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9589388.1"
                     /translation="LAMIAKRIETSRAKLDNPSKPIGVFMLAGPSGVGKTETALALAE
                     ALYGGEQNMITINMSEFQEAHTVSSLKGAPPGYVGYGEGGRLTEAVRRKPYSVILLDE
                     VEKAHPDVHEIFFQVFDKGIMEDGNGRRIDFKNTLIILTTNVGTDLIMGLSRDPKYRD
                     DPEELGLRLRPELLEVFPAALLGRIVTIPYFPLTNAMLDGIVRLQLGRIDQRIRDNHG
                     ASFVYADAVIDHIVSKCNDPDSGGRMIDNIITNTLLPALSREFLKKSLAKEEITQARV
                     TIEDNDFAYAWT"
BASE COUNT         1841 a         2972 c         2816 g         2013 t
ORIGIN      
        1 gccaaaaaat tcacggcaga gaccggcgtg ccggtggagt tcatcaccgg cggcaccatc
       61 gaccggctca acaaggccaa cctcgccaag ggcaatcccg agagcgacct gaccttcacc
      121 acctcgcatg tcggctggat ctacgccacg gacggattgt tcgaagaact cgatacgacg
      181 aaaattccaa acgccaagaa ccttgccgac gaagcccgca tcagcccgtt ccatctcggg
      241 gcctgggcct atgtctacac catcggctat cgcgccgacc tgctcggcgg catgaaattc
      301 gagagctggg aagatctctg gaagcccgag ctgaaagcca agattgcggc gcccgacttc
      361 gatccgagcc acatcatcgc gatctcggcg atcctgtccg gcggcgatgc cgcgcattgg
      421 gaaaagggcc aggacaagct gaaggcgctg aagccgaact tcaaggcgtt ctataccaac
      481 gacgccaaca gccagcagct gctggcgtca ggcgaaacgc cggtccaggt cgtcctctcc
      541 atgaatgctt attacatgat ctcgcagggc gtaccgatca cgctcgcgat cccgaaggag
      601 ggcgcggtgc tcggcgtcga taccgtggcc atcatgaagg gctcgaagaa atccgaactg
      661 gcctacaagt tcatcaacgc gctgtacgac cctgaggtcc aggcggaaat cgccaagatc
      721 aagaagggca gccccgcggt gctcaacgcc aagatcgatc cggaaatcgc gaagctgccc
      781 ggcgtcttca ccaccgccgc gcaatggaag cagcagatca acatcgacgc caagctgcgc
      841 gccgagaaga ccgcggaatg gcgcaaatgg ttcgccgaga acatcatgaa ctgatggcga
      901 gcgccggctt gttcctgtga acccgcagcc cttcctccgc ccgttcaccg ctccggccgt
      961 gctgtgtacg gtcggcctgc tggccgcgat ggcggcggta ctgcagttca gttttcgcgc
     1021 ttacgtgcct ggctctctgg agccgggcgg cttcacgctg gcgaattttg cggacttgct
     1081 gaaaccgctt tatgcgcggg tgtttctcga caccgtctgg atctgcttcc tgacggcgat
     1141 ctcgacgttg atcgtcggct atcccctggc ctatgcgctg gtgcgaacgc agaacgccat
     1201 cctcaagtcg accctgttgg tgatcgtcgt cacgccgctg ttcctcggcg aagtcgtgcg
     1261 tacctattcc tggatcatcg tgctgggcaa taacggcttc atcaattcgg tgctgatcta
     1321 tctcggcatc gtcgaacagc cgttccagtt catgttcacc accttcggcg tggtggtcgc
     1381 gctggtgcat gttaccttgc cggtgatggt gatcatgctc gccgccggcc tgtcgcatat
     1441 cgatcgcgat taccagaagg ccgccgaaag cctaggcgcc ggccccgtcc gaacgttcct
     1501 caccatcacc ctgccattgt cgatgccggg cgtcgtcgcc ggcgtcacca ccgccttcgc
     1561 ctggaccttc agcgccttcg cctcgccgca actgatcggc ggcggcaagg tcaacatgat
     1621 ctcgaacctc gtgtaccagc tcggcttcgc cagcttcaat ttcccgtttg ccgccagcct
     1681 ctgcgtggcc ggactggtgt tgacgatcgc ggtactatcg atgctgcggc tggcggcgcg
     1741 cccgctcgac aatattcggg cgcattgaga tgccgcgttc gacctaccag ctcgggctcg
     1801 gcttcgtcgg caaactcgcg gtgctgctga tcctcggctt tctggtgctg ccggtggccg
     1861 tcgtcaccat cgccgctttc aacgacaggg cgatcctgtc gtttccgccg caggcatggt
     1921 cgctgcgttg gtttgccaag gccatcgcct atcaggattt tcgcgacggc tttcgcaacg
     1981 gcctgatcgt caccgcctgg agctcgacga tcgcgctgct ggtcggggcg atgtttgcct
     2041 tcgcgctcga ccgctatcgc tttcgctttc gcaagctgat cgaagccatt ctgctgtcgc
     2101 cgctcgtcat tccgcatttc acggtcgggc tcggcttcct catcctcgcc gcgcagatcg
     2161 gctggacccg cggttacgcg gtggtgatat tctgccacgt cgtgctggtg ctgcccttcg
     2221 tgatccgcag cgtttatgtg tcgttgcgca atctggatca gagctatgaa ctcgccgccg
     2281 ccagcctcgg cgcgccaccg ctgcgggtgc tgctcacggt cacgctgccg ctgctgctgc
     2341 ccggcctggt cagcggttgg ctgttcgccg cgatcctgtc gttcaacgaa ttcacagcgt
     2401 cgctgttcgt cacggcgcag cggacgcaga ccctgccggt cgccatgtac aactatgtgc
     2461 gggaatatgc cgatccgtcg atggcggcgc tgtcggtgat gtacatcgtc ggcacggctg
     2521 cgttgatcat cctcgccaat gcatttctgg gtctcggtaa ggtactgaac attgaacata
     2581 ctcgctgaca ctggcgttgc ggcgcagaca gccgggttcg ctgccggcga tgacgttgcg
     2641 gtccgcttcg actccgtgac aaagcgtttc ggcgaagcag tcgcgctgcg cgaggtctcg
     2701 ctgtcggtcc ggcggggcga gttcatgacg ctgctcggcc cgtccggatg cggcaagacc
     2761 actttgctca atctagccgc cggtttcctc agcccggacg gtggcgacat ccgcatcggc
     2821 ggcgaacgtg tcaacgacgt gccgacctac aagcgcgaaa ttggcatgat gtttcagaac
     2881 tacgcgctgt tcccgcatat gtcgctggcc gacaacatcg cctacggcct gaaggttcgt
     2941 cacgttccga agaaggagag ggaccggcgc gttgaggagg tgctggcgct ggtccggctc
     3001 actggcctcg aaggccgcaa gccgcgccag ctttccggcg ggcagcagca acgcgtggcg
     3061 ctggcgcgcg cgttggtgat caatccgacg gtgctgctgc tcgacgaacc gttttcggcg
     3121 ctcgacaaga atttgcgcgc atcaatgcag gtggagcttc gcgagatcca gcgcaagctc
     3181 gaggtcacga cgatcttcgt cacccatgac cagggcgagg ccttgagcct gtccgaccgg
     3241 ctcgcggtca tgtcggaagg gcgcattcgc caactcggta ctccggaaga aatctatcgc
     3301 cggccctgcg accgcttcgt cgcatccttt gtcggcgacg ccaatgtgct gcgcggaagg
     3361 cttgatggcc tcgacggagc ccatgccgtc gtcgccgtgg gcgccgcgcg tgtcaaggtg
     3421 ccggcaagct cgttgcgcgg cgtcacggca tctgcgccgg tcgacctgtt tgtccgcccc
     3481 gaacagttgc aaatggtcga ctccgacgcg ccctgcgcca ccagcggcac ggtcgccgcg
     3541 caggtctacc agggcgggca cgtcgatctc tacatcgact gcgctgaatc ggtgcgcgac
     3601 cggctgctgg tgcgatcggc gcgacatgac gcgatgacgc gatggccgat cggcgcacag
     3661 gttgggattt ccatcggtga cgacgacgcc gtcgcgttcg cgaccggata gcgcgattca
     3721 acgttgcgct tcggatgcgg ctgcaagcgg ccgccgcatc cgaagcgccg tcgcgccccc
     3781 gggcgcgatg tgttgaatat agttcgatga ccaataccgc ttggccgctt cgtagtcccg
     3841 ctggttgaaa agcgtttcga acgcttcgag cattgatgcc tttgattctt cggttgtctt
     3901 cgccatgttg tgcacacttg cattttcgga tggttatttc ggacggcttt tcggtcatgg
     3961 cgtttctcga ccatgtccgt cggcgtgctg gccccgctag gccccgacgc taagtcggct
     4021 cgaaacgttc tcgtaccaac gctccagcgc ggcgtgctcg ccgggcgccg aaaaaaactc
     4081 acggctttgg tcgccaaaga cagtgaccag tgcggcaatg tctgccgccg agaacgcgtt
     4141 gccgtcgaaa aaaggggact ttttgccaga cgggtatcga gaccggcgta gaagtttttc
     4201 acccgaagct tgctgcgttc ggccagcgct agaaccagcc gccgacaata ggtcgccggt
     4261 tcgctttggt cctagtctct gtcacctccg tccaagccgc acatgggggg caatactggt
     4321 atcagccgac cggttgacgc gcgcattcga gatttccctc aagagtacgt ccgaacttga
     4381 agcgtatcgg acggaggttt ggcttgagag tattggctct cgccagcgtc ggcgaaaaat
     4441 ccagcgcgcg ggcaaaaaca tatgtggcgg gcccgaagct gattccgcga cgacacgcgc
     4501 tgatgcaggt gctgctgatt gcttccttcg cgatgagcgg gcgcgccgag gcggcctgca
     4561 cgccggcctc gccggtcaac aacacgatcg tcacctgcac cgggaagacc accaaccaga
     4621 acggcaccaa cggctacggc acggttaacg acaacaacaa tacctacaat atccaggctg
     4681 gcgcgacggt gacgggagac ggtttcggcg tggtgttcgg tacgggggca acgatcaaca
     4741 atgacggcac catcacggct ggtattggtg tcagcggtag cgatgcgacc atcaacaata
     4801 cgagcaccgg cgtcatcaca ggtgacacaa acggcatcca ggccacgacg gcaaccgtca
     4861 ggaatgccgg cctcatctct gcgatgggcg tctctgcgac cggcgggaat ggctttggca
     4921 tctctgccga caccgccgat gtgagcaaca cgagcaccgg ccatatcacg ggaacattca
     4981 ctggaatcgg cacctcgact ctcgctgccg tcaacaatgc cggcgctatc tcaggtggga
     5041 ggctcggcat taccggcgac accgcccgcg tgatcaactc tggcgccatt gccgcaggcg
     5101 ctgccgccat ttttgccagc accgccaatg tgaccagcac cggcgccggc accatatcgg
     5161 gtgggacgtt cggaattgtt gcaaacaccg tcgcctccat cgccaacgcc ggcatcatct
     5221 tgggtaacat cggcattcaa gccagcggcg ccgccaccat caccaactcg agcgccatca
     5281 tctctaccgc cggcgcaagc ggcacggcaa tcaagctgtc gaacgctgcc gacacgctga
     5341 cgctgctgac cggatcgcgc atcgtaggcc tgctcgacat gggttttggc aacgacacgg
     5401 tgaatacctt gaccgtcgcg ccgggcagca aggtgtcgtt gcccacgacc gctgctgcgc
     5461 tgccaaccct catcaatttc agcggcgtca ccaacacgag cttcaccggt ggccgcttca
     5521 ccgacccggc ggtgcaggcc ggcaaccacc tcgcgacgct cgatcccacc gcgctggcgg
     5581 cagaccggcc gcggcttgat cgacctcacc ggcaggcgtg ccgtcgctgg tgcagggacg
     5641 cttcaatggc gtggcgccgt ccgcgaacac tccgagcgca ccaaggtctg aggcacccct
     5701 tggcgcttaa gggatcgaaa aacgacagat aatagtgagg ctcgttcctc tcagcctcaa
     5761 ttttcccaat ttctgtgagc cgcgtcgagc agttgccgcc aattatccgg ttgctagggc
     5821 tagcccttgg atggacgccc gccaaattgc cgagcaagcg gcgatttgcg aacgtcgcca
     5881 atttcctttc gcgtcaaggg cgcgccggga accgcgaggc cggggttcat caaatcccgt
     5941 atgtcacctt ggtgatcgag ccagagcagg tgcccgaact cgtgcgcgag cgacgtgtcg
     6001 ttggcgtcgt ctccgaccgc gcagacgcgg agctcctttt tggaggcgcc cccctctgag
     6061 cccacaaacc gacgaaccag cagcaggctg acggcattct tcattgggaa gtccttggcc
     6121 agcatgagga aaccctcatc gtcaaggtct tcggatccct tcggcgcctc aacgagatcg
     6181 gatgtggtat accgcagccg aaattcgatg tcagcggcgc cgaatatctt gttcacttcc
     6241 ttgaacgacg atatcaggga ggccgcgctg cgtgtcgttt tgatctcacg cgtccgttga
     6301 atgcgttcgc gctgcatgcg ggtctcgcgg cccacacgca ccgggtgttc cacaatctca
     6361 ggcgtcgaaa tcgtcagggt cagcagatga gcgtccaccg gtatgatgac cacattactc
     6421 tccatgcatt agagtttgct caaccgatcc aggaaaccgc cccgtgcgat catgtagtga
     6481 atatccttcg ggaagggaaa cgcctcgcga acgttcgtga tcgcctgtcc gagaaatttc
     6541 ccctgcggcc atgacttgtg catggaagga gctccgtcgg ccgtcggcgg aatcatgaaa
     6601 cggtcgacga aggaggggtc gagatcgttg accttgacca tctggccgaa tgccggaaat
     6661 gcgtcgtagt ggccgatgct cacattgaga ttcttgagcg cggggcgacg cggctcagcc
     6721 ttgttgcctt ccggcgtcgc cagcccacgc cccgtcaggt ccagcttgac ggattgcgga
     6781 agggtatctg gaccatagat gatatacagt ggcctggcgt ttggtcccga caaccatcga
     6841 taccagaatg tggcgtggtc gggcggtccg ctcccgtcgt agagacagtc aaacccccaa
     6901 catgccttaa ggttcgactg atattttccc aggctcccga caagattgcg cataccgttg
     6961 ccgcctcctg aatggcatgc aatgacgagc ttgccgattt gaagttgagg cggagacttt
     7021 aaatcgggaa cctggaagtg cgaaatagct gcaagtactt cgttcaggta gcgttcgccc
     7081 cagttcggtt tcgccaggtc cttagcgctg tagtttccag caaacttatc tttgacgaaa
     7141 tattcgaaac ccaggaacgg tgcgataagc accacattct tgttggaatc gcgaacctgt
     7201 tcgcgtatcc gcgcaggatc gttgcggaac agaaacttat ggtctctgac agcgaatcca
     7261 tgcagccaga tcacgacgtc gagcatgttg cctagcgccg cgggtttcgg cggaagatag
     7321 acggccgtcg gcttgatgca gtgcgcggtc cctttcgtga acagcgtcac gcccggaaag
     7381 ttgccgatgt cgtcctcttc cagttcacaa cgcacgcagg ttgctccgtt tcgccatgtc
     7441 acgcgcgcgg ctcggacagg ccgatgtcat gctctgctgc ctcggcaacc cctccaaggg
     7501 ttcatctgta ttgctaccaa atcggcacac cggatgtgtc ctgtgagtac ttcatgtaag
     7561 ttgcataaag cgatttggga tcgagcaatc gggttctaat gtccttatag aacatgccgg
     7621 cgagcggacc taaactgtcg gacggcgcgc cagacacgct aaagaaatcc agaccggcca
     7681 caacggatac gcgcttaatc tgtttgttca tctcttggat ggattggaaa atttcgcttt
     7741 ccgcgaatct caactcattc ccgattagac catgacaatc gtcgatgttt cgctgcccca
     7801 cgcacttctt gatggcgttc cgaaagggat aattgcgcca cggggtgtcc ggcgcggctg
     7861 aaaagctcac gcctcggctc atactgccgg tggtgccgtc gaacggcagt ttgccggctg
     7921 tccttgtcgt catcgccttg cggcggccat cggtggtgtc ccagtttacg tactcgaata
     7981 cggccactcg attgtagtac cagagatcca gaaaccccgg atatccaatc tgctgggcga
     8041 gcttgatcca gtccctaagt cgtttctgac cgtcgccgct ccaatgtggg ttttcgacga
     8101 cgatcgctgc gtgctggaga accagttcag cgagattctt tcccgactgt tcggcaacag
     8161 ccggcgaagc gtagatttgc ccgccctcgt tttggctgac agcgattact ggtcgaccga
     8221 actgaacgct catcatccag cgcagggtcg gatcgggatt ggcgcgccgg taaaggcggg
     8281 cagccacact aactctggtc ccatttgcaa ttgaattttc aagcagctcg cgatgaaaat
     8341 ttgcaagctg tccggtctgg cgcacaaggc ctcgggcgaa attgccgccg tcggttctat
     8401 agacgaccaa atggcgcaac tcgtaagcaa cgttcgcggc aaagcggtcg aagttcgtat
     8461 caaacttaac tacaagcatt ctggtttcgc ccagcggttg gtcgccgcgc gcaatgtgtt
     8521 cttgggcgcg tttttttaat tcctccatcg ccatgtaatc agtacgttgc gatccgtcgg
     8581 ccatgtgcat atctccaggt gatctgtaat cggatttcag acgaactaga acccgtcgaa
     8641 cgctactcct cagaagtcag cgacttgaga gtctccagca cccgtcgggg acacgggaat
     8701 gtcgtcccag aaccttgacg tcgttgtttc atgggatagc gccatcaaag tcacatgctc
     8761 atgacgacaa atcgctcaag tccaggcata cgcgaaatcg ttatcttcga tcgtcactcg
     8821 ggcttgcgtg atttcttcct tggccagaga tttcttcaag aattcccggg atagtgccgg
     8881 cagcagcgta ttggtgatga tgttgtcgat catgcgaccg ccggagtccg ggtcattgca
     8941 ttttgaaacg atatgatcga tcaccgcgtc agcgtagacg aatgaggcgc catggttgtc
     9001 gcgaatgcgt tgatcgatgc gaccaagctg tagccggacg atgccatcga gcatggcatt
     9061 cgtcagcggg aaataaggaa tggtcacaat gcgcccgagc agcgccgccg gaaatacctc
     9121 gagcagctcc ggccgcagcc tcaaaccaag ttcttccgga tcgtcacgat atttcgggtc
     9181 gcgcgacaac cccatgatca agtcggtgcc gacattggtg gtcaggatga tcagggtgtt
     9241 cttgaaatcg atccttcgac cgttaccatc ttccatgatg cccttgtcga acacctggaa
     9301 gaaaatctca tgcacgtcgg gatgggcctt ctcgacttcg tcgagcaaaa tcacgctata
     9361 cggctttcgc cgtaccgctt cggtgagccg gccaccttcg ccgtagccca catagcctgg
     9421 aggtgcgccc ttcagcgaag agacggtatg cgcctcctga aactccgaca tgttgatggt
     9481 gatcatgttc tgctcgccgc catagagcgc ctcggccagc gccagcgcgg tctcggtctt
     9541 gccgacgccg gaggggccgg cgagcatgaa tacgccgatc ggctttgatg ggttgtcgag
     9601 cttggctcgg ctggtctcga tcctcttggc aatcatcgcc aa
//
LOCUS       DASWGM010000589         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_901042,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000589 DASWGM010000000
VERSION     DASWGM010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901042"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1842)
                     /locus_tag="VGA15_16790"
     CDS             complement(172..1842)
                     /locus_tag="VGA15_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX9589389.1"
                     /translation="MGTCAMDKNEGLPFFDVIILGAGWSGLMACKYCLGEGLKTLVLE
                     RRDSIGGVWSFTQDGRYGGVMTTTETTSSRCVTEMSDFPMPESYPEFPSHGHILAYLK
                     AYRDRFALERHIRLDHRVDSARKAGDVWHVTCANGSKFRAKNLIVSSGVHQHPNDVSG
                     DAPFNRFEGPILHSAAVKEAPPDLAGKTVVMWGGGESASDIAFQISKTAARIYWCIPN
                     GQWFVPKVVNRWWPFPSKRPKVGDQASSRVRLLLSPTRGFSPFINQFLQFSLGFNGHG
                     QEAWRTHAPYHQSFFNKSYEVMPQVKSGKIVAKRDIERCEGNRIYFTDGSAVRADVMV
                     TCSGYKLVAPFFEPGMKADPQQCYKYLFGTEPSVAFVGFVRPVIGSIPAIAELQSRYA
                     AMVFAGRRALPPLADRERITRADTAFWNHRFRFTSRRLRGLVDMPYYNDQLARLIGCR
                     PRFWKLLFTAPRKWWWVVSSPWNGCQFWLNDESHHERIFATFARYRFNQASEVYILLA
                     LAPILPFVGLISRIRLFINEHFVWRKQSAPRSVVCAAAADRRPAERAA"
     gene            complement(2020..>2319)
                     /locus_tag="VGA15_16795"
     CDS             complement(2020..>2319)
                     /locus_tag="VGA15_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589390.1"
                     /translation="SEPYYDESMLCATVEGSLPIAGGVEAADRAAIEAARDPAAKRAE
                     IEAKLEARPSPMRTAEAFAVEEIIDPRRTRSYLTRFAGLAAPLRTAGRAHFGCRP"
BASE COUNT          445 a          763 c          723 g          388 t
ORIGIN      
        1 tgcggatcag atggatccac ttgtaccagg cgtcgatccc gcaatagtcc gtcgaatcca
       61 tgcggatctc gaccatggga tcgagaatct cccgcgctgc cagccgcggc gcttcgccgt
      121 tgacgacccg aaccagcgcg agaaccaggt cgatcggcga cctgctctcg atcacgcggc
      181 gcgctccgcc ggcctccggt cggcagcggc ggcgcacacg acgctccgcg gcgcggattg
      241 cttccgccac acgaagtgct cgttgatgaa caggcggatg cggctgatca gcccgacaaa
      301 tggcaggatc ggcgccagcg ccagtaggat atagacctcg ctggcctgat tgaagcgata
      361 gcgcgcgaag gtggcgaaga tgcgctcgtg gtgggactcg tcgttgagcc agaattggca
      421 gccgttccac ggcgacgaga ccacccacca ccatttgcgc ggcgcggtga acagcagctt
      481 ccagaaccgc ggacggcagc cgatcagccg ggcaagctga tcgttgtaat acggcatatc
      541 caccaatccc cgcagccgcc gcgacgtgaa gcggaaacga tgattccaga aagcggtgtc
      601 cgcccgggtg atgcgctcgc gatccgcgag cggcggcagc gcgcgccgtc ctgcgaatac
      661 catcgcggcg taacgcgact gcagctccgc gatcgccgga atggagccga tgaccgggcg
      721 aacgaacccc acgaaggcca cggatggttc cgtcccaaac agatatttgt agcactgctg
      781 cgggtccgcc ttcatcccgg gctcgaagaa cggggccacc aatttgtacc ccgagcaggt
      841 gaccatgacg tcggcccgca ccgcactgcc gtcggtgaaa taaatccgat tgccctcgca
      901 acgctcgatg tcgcgcttgg cgacgatctt cccgctcttc acctgcggca tcacctcgta
      961 gctcttgttg aaaaacgact ggtgataggg cgcgtgggtt ctccacgcct cctggccgtg
     1021 gccgttgaag ccaagggaaa actgcagaaa ttggttgatg aacggcgaga agccgcgcgt
     1081 gggcgacagc aacagccgca cccgcgacga cgcctggtcg ccgaccttgg ggcgcttgct
     1141 gggaaacggc caccaacggt tgacgacctt cgggacgaac cactggccgt tcggaatgca
     1201 ccagtagatg cgggcggccg tcttgctgat ctgaaacgcg atatcgctgg cgctctcccc
     1261 gccaccccac atcacgacgg tcttgcccgc gagatccgga ggcgcctcct tcacggcggc
     1321 gctatgcagg atcggcccct cgaagcggtt gaagggcgcg tcgcccgaca cgtcgttggg
     1381 atgctggtgc acgccggacg acacgatcag gttcttggcg cggaacttcg aaccgttggc
     1441 gcacgtgaca tgccaaacgt cgccggcctt gcgcgcgcta tccacccgat gatcgaggcg
     1501 tatgtgccgt tcgagagcga agcggtcgcg gtaagccttg agataggcga ggatgtggcc
     1561 gtgggacgga aactccggat agctctccgg catggggaaa tccgacatct cggtgacgca
     1621 acgcgacgag gtcgtctccg ttgtcgtcat cacgccgccg tagcggccgt cctgggtgaa
     1681 cgaccacacg ccgccgatcg aatcgcggcg ttccagcacc agcgtcttca gtccctcgcc
     1741 caaacagtac ttacacgcca tcaaccccga ccagcccgca ccgagaatga ttacatcgaa
     1801 gaacggcaga ccttcattct tgtccatggc gcatgtaccc acgcaacgct cctaaagccc
     1861 cggggcgaca cggtcagact actggcgtca agcgttacgc gtcaggcccc tctagaaatg
     1921 gtttcccaat cctaagtcgc gctcagtatg cacagccctt gtgctgctga caatgctttc
     1981 cgcaactttc cgcatgcacc gagaaggcga tcacaatact cacggccggc agccgaaatg
     2041 tgcgcggccg gcggtgcgca agggtgccgc cagccccgcg aagcgagtga gataggagcg
     2101 tgtccggcgc gggtcgatga tttcctcgac ggcgaaggcc tccgccgtcc gcatcggcga
     2161 tggccgggct tcgagcttgg cctcgatctc ggcgcgcttg gcggccggat cgcgggcggc
     2221 ctcgatcgcc gcgcggtcgg cggcctcgac gccgccagca atcggcagcg agccctcgac
     2281 ggtcgcgcaa agcatgcttt cgtcgtaata tggttcgct
//
LOCUS       DASWGM010000590         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_180426,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000590 DASWGM010000000
VERSION     DASWGM010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180426"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VGA15_16800"
     CDS             <1..556
                     /locus_tag="VGA15_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589391.1"
                     /translation="LDPGLVIGRLPHEFSGGQCQRICIARALVLNPEFIICDEPVSAL
                     DVSIRAQILNLLEDMKARFGLTLLFIAHDLAVVKAVSDRVAVMYLGRLCEVGPSEQLF
                     ARPAHPYTALLIEAIPVPDPDVRPAESVAVGEPPSPIAPPSGCRFRTRCPRADQRCSD
                     EVPELREVAAGQFAACHHPLIGAA"
     gene            629..1693
                     /locus_tag="VGA15_16805"
     CDS             629..1693
                     /locus_tag="VGA15_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX9589392.1"
                     /translation="MKSFQVTDFNAPLTEVDQPTPQPSGTQVLIKVKAAGVCHSDLHI
                     WEGGYDLGHGRKPLSLKDRGVSLPRTMGHETAGEVLAFGPDVKGDTGGLKVGDVALVY
                     PWLGCGKCETCLADEENMCLKPNSIGVYCDGGYADHMTVPHPKYLLNLRGLDPVTAAP
                     YACSGVTTYSALKKVESSFNTPIVIFGAGGLGLMALSLLKAMGGKGAIVVDIDARKRE
                     AAEQAGALATVDGKAPDALEQLAQKAGGPIRAVIDLVGNAATTQLGFDCLTKGGKLVI
                     VGLFGGGAPWALPLIPIKAITIQGSYVGNLRETQELLDLVRAKKIGPIPVTPMPLAKA
                     NEALIDLQKGRLVGRAVLTP"
     gene            1783..2862
                     /locus_tag="VGA15_16810"
     CDS             1783..2862
                     /locus_tag="VGA15_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX9589393.1"
                     /translation="MSANNALHVAVLAGDGIGPEVMAPALEILRKIEATSDLKFRFTE
                     APAGANNYLATGKSMPESTVRLCDEADAILLGACGLPAVRYPDNTEIMPQVELRFHFD
                     LYAGVRPARLIPGVPSPIVGADQRGIDLIVIRESTEGLFASMGKGVVTDTEARETLVI
                     TRKTSERLFEFSFRLAERRKARGRPGQLTCVDKANVFKAFAFFREMFDEAAKHHPEVK
                     ADRLYVDACSALLVKRPWDFDVMVMENMFGDILSDMTAGLIGGMGMAPSADIGDRHAV
                     FQPCHGTAPDIMGQGKANPTGMILSAAMMLDWLADKHGFEGAAEAGERIERAVDKVYA
                     AGIKPMEFGGRNGTADIARAVLEAL"
BASE COUNT          639 a         1180 c         1161 g          659 t
ORIGIN      
        1 cctcgatccc ggtctggtga tcggacgcct gccgcacgag ttttccggcg gacagtgcca
       61 gcgcatctgc attgcccgcg cgctggtcct caatcccgag ttcatcatct gcgacgagcc
      121 ggtatcggcg ctcgacgtct ccattcgcgc ccagattctc aatctgctgg aggatatgaa
      181 ggcgcgcttt ggtttgacgc tgctgttcat cgcccacgat ctggcggtgg taaaagcagt
      241 cagcgaccgg gtcgcggtga tgtatctcgg ccggctgtgc gaggtcggtc cctccgagca
      301 actgtttgcg aggccggcgc atccctatac cgcgctcttg atcgaggcta tcccggtgcc
      361 cgatccggac gtgcggcccg ccgaaagcgt ggcggtcggc gaaccgccgt cgccgatcgc
      421 gccgccctcg ggctgccggt tccggacccg ctgcccccgg gccgatcagc gctgcagcga
      481 cgaggtgccg gagctgcgcg aggtcgccgc tggccagttt gccgcctgcc atcatccctt
      541 gatcggggcg gcgtaaacat ctagcaactg cttgggaacg cggggcaacg tccgggcaag
      601 acaagaaaat tatagcggga gcgaagcgat gaagagtttc caggttaccg atttcaacgc
      661 ccctttgacg gaagtggatc aaccgacgcc gcagccgtcg gggacccagg tcctgatcaa
      721 ggtgaaggcg gccggggtct gccacagcga tctgcacatc tgggaaggcg gctacgacct
      781 cggccatgga cgaaagccgc tctcgttgaa ggaccgcggt gtgtcgctgc cgcggacgat
      841 ggggcatgag accgccggcg aagtgctggc gttcgggccg gatgttaaag gcgataccgg
      901 cggtctgaag gtcggcgacg tcgcgctggt ctatccttgg ctcggctgcg gcaaatgcga
      961 gacctgtctc gccgacgaag agaatatgtg tctgaagccc aattcgatcg gcgtctattg
     1021 cgacggcggt tatgccgacc acatgacggt gccgcatccg aaatatctcc tgaatctcag
     1081 gggtctcgat cctgtcaccg cggcgcccta tgcctgctcc ggcgtcacca cctacagcgc
     1141 cctcaagaag gtcgagtcga gcttcaacac gccgatcgtg attttcggcg ccggcggtct
     1201 tggcttgatg gcgctgtcgc tgctgaaggc gatgggcggc aagggcgcga tcgtggtcga
     1261 tatcgatgcc cgcaagcggg aagccgccga acaggccggc gcgctcgcga ccgtcgacgg
     1321 caaggcgccc gacgcgctgg aacagctcgc ccaaaaggcc ggcgggccga tccgcgcggt
     1381 gatcgatctc gtcggcaacg ccgcgaccac gcaacttgga tttgattgcc tgaccaaggg
     1441 cggcaagctc gtcatcgtcg gcctgttcgg cggcggcgcg ccgtgggcgc tgccgctgat
     1501 cccgatcaag gccatcacca tccagggcag ctatgtcggc aacttgcgcg aaacgcagga
     1561 actgctcgat ctcgttcgcg ccaagaagat cggaccgatt ccggtgacgc cgatgccgct
     1621 ggccaaggcc aatgaggcgc tgatcgacct gcaaaagggc aggctggtcg gccgcgcggt
     1681 gctgacgccg taacgtcatt gcgagccaac gggtcgcgcg aatgcgcgcc ccgttggctc
     1741 gcaatgacgg tatcctctct acggatctca tgaggaattc ccatgtccgc taacaacgcg
     1801 cttcatgttg ccgtgcttgc cggcgacggt atcggccctg aagtgatggc tccggcgctc
     1861 gaaatcttgc gcaagatcga ggcaacgtcg gacctgaagt ttcgcttcac cgaggcgccg
     1921 gccggcgcca acaactatct cgcgaccggc aagtcgatgc cggaaagcac cgtgcggctg
     1981 tgcgacgaag ccgacgcgat cctgctcggc gcgtgtggcc tgcctgcggt gcgctacccc
     2041 gacaacaccg agatcatgcc gcaggtcgag ctgcgctttc attttgatct ttatgccggc
     2101 gtgcgtccgg cgcggctgat cccgggcgtg ccgagcccga tcgtcggcgc cgaccagcgc
     2161 ggcatcgatc tcatcgtgat ccgggaatcc accgaaggcc tgttcgcctc gatggggaag
     2221 ggcgtcgtca ccgataccga agcccgcgag acactggtga tcacgcgaaa aacctcggag
     2281 cgattgttcg aattttcctt tcggctggcc gaacgccgca aggcgcgggg ccggcccggt
     2341 cagctcacct gcgtcgacaa ggccaatgtc ttcaaggcct ttgccttctt ccgcgagatg
     2401 ttcgatgagg ccgccaaaca ccatcccgag gtcaaggccg accggctcta tgtcgacgcc
     2461 tgctcggcgc tgctggtcaa acgcccctgg gatttcgacg tgatggtgat ggagaacatg
     2521 ttcggcgaca ttctctccga catgaccgcc ggcctgatcg gcggcatggg catggccccg
     2581 tcggccgaca tcggcgaccg ccacgcggtg ttccagccct gccacggcac tgcgcctgac
     2641 atcatggggc agggcaaggc caacccgacc gggatgatcc tgtcggccgc gatgatgctg
     2701 gactggctcg ccgacaagca cggttttgaa ggcgccgcgg aagccggcga gcgcatcgaa
     2761 cgcgcggtcg acaaggtcta tgcggcagga atcaagccga tggaattcgg cggccgcaac
     2821 ggcaccgcgg atatcgccag ggcggtgctc gaggcgttgt aggcgcaggc gcggcggaat
     2881 atccccgaag cagtcattcc ggggcgatgc gaagcatcga acccggaatc tcgagattcc
     2941 gggttcgcgc tggcgcgcgc cccggaatga cggagtctga attgagcgtc gcatcgctcc
     3001 ctcgcaaaga tcgtcatact ccgaaagcgg agtatccagt acacggcggc ttctcgcttc
     3061 catcattggc gtctctggaa tactggatcg cccgctggag cctgtcatcg ggcggcgcat
     3121 tcgcgcgacc cgttggctcg caatgacggt gccctagttt caaacgcaca ttcgcgatct
     3181 cgcgacgcga tgcgcccgag gcgttgctga agctcacgcc caaagagggc gtggggaacg
     3241 ccgggtgccc gctgcacccg cggcctcgcg tgcactttgc attggtagaa cgcacacgag
     3301 taaacgagta caccggaatc acccggcatt cccgcacgca atggttttaa cggtttcctt
     3361 cgtgctctcc ccggtgaccg ggcttgtttg ccaccgtcgt cagcggatta aggttttgtc
     3421 tgcgcccggt agggccgact tgacctccgc gtacttgacg ccagcgtcgg ggcgtcagga
     3481 ccacacgact tcgccgtccg catcagcgcc gttcgtcagc tcgccgtttg atcgctcacg
     3541 gatccctaaa gaacccgccc tgcatcacgt tgcgcgccta acactgccgc gtccaccgca
     3601 tcccaccccg cgtccgtgac gatcgcgata cgcccctct
//
LOCUS       DASWGM010000591         4596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1859945,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000591 DASWGM010000000
VERSION     DASWGM010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859945"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VGA15_16815"
     CDS             complement(<1..617)
                     /locus_tag="VGA15_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9589394.1"
                     /translation="MAASVSAQEVKHYRFAYDQPKTTGYGILGDIFSDKLKALSKGTM
                     LIEQYPGAQLGQEPQVLQLVKSGDVEFCISSSANAATLSPQAGVMSMHFLFRSEAHLV
                     KAIADPEVSKAVKAMIAETVQGAHVIALSTLGLRNMYSKREIKNVADMKGLKVRVQAT
                     PTEDTMFPAYGAQIVHMPFGSVYTSLQTGVVDVAENGVNVYLANK"
     gene            complement(859..2610)
                     /locus_tag="VGA15_16820"
     CDS             complement(859..2610)
                     /locus_tag="VGA15_16820"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX9589395.1"
                     /translation="MADGLRKGLTSYGDAGFSLFLRKAFIKAMGYSDDALNRPIVGIT
                     NTHSDYNPCHGNVPEIIEAVKRGVMLAGAMPMVFPTISIAESFAFPTSMYLRNLMAMD
                     TEEMIRAQPMDSVVVIGGCDKTLPAQIMAAVSADLPTVVIPVGPMVVGHHKGEVLGAC
                     TDCRRLWAKFRAGEIDEAEIEAVNGRLAPSVGTCMVMGTASTMACITESLGLSLPMSA
                     TIPAPHAERFRSAEASGKVAAEMAKAKGPKPSEFLTPAAFRNAQVVLQAIGGSTNGLI
                     HLTAIANRTEHRIDLEAFDRLGREVPVLIDLKPSGAHYMEHFHHAGGVPKLMAQLGDL
                     IDLDAKTITGASLRDVVAGAEEVPGQDVIRARNSPIKPEGAMAVLHGNLAPRGAVIKH
                     SAASPKLLRHTGRAVVFDSVEDMTLRVDSPDLDVTADDVLVLRNAGPKGAPGMPEAGY
                     LPIPMKLARTGVKDMVRISDARMSGTAFGTIVLHITPESAVGGPLALVRNGDMIRLDV
                     AKRSIDLLIDAAELEKRRAALAPIATPEWARRGYAHLFNETILQADEGCDFDFMRGRG
                     KGVVILRCEPTWASLEG"
     gene            complement(2754..3572)
                     /locus_tag="VGA15_16825"
     CDS             complement(2754..3572)
                     /locus_tag="VGA15_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9589396.1"
                     /translation="MPRILMTGASGGIGTRLRKLLPPIYPHLLLSDLKAPADLGAHEN
                     FRAAELSDPAQVEAICDGVDGILHFGGYSVEGPWDDILQSNIIGGYNLFEAARKKGVK
                     RVVFASSNHAVGFYPRHHHIGTDVTARPDSRYGVSKVFGEAVGALYADKHGLGVTCIR
                     IGNFGDKPLDHRRLSIWLKPEDLVQLCRIGLDHPDIHFEIFYGASYNERAWWDNHRAY
                     DLGYRPTGLAEDFREHAFAEQAKLPPDPVGDFYQGGAFCGMEFDGDMSRIVDLK"
     gene            3850..>4596
                     /locus_tag="VGA15_16830"
     CDS             3850..>4596
                     /locus_tag="VGA15_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9589397.1"
                     /translation="MSETVSHSSGWRPATYYPDPAIHALDPRFEKYWLKLSAVERLTT
                     GLRWAEGPVWFGDGRYLLCSDIPNNRIIKWEEETGTVSIFRKPSNFANGHTRDRQGRL
                     ITCEHGGRRVVRTEYDGSITVLMDSLDGKRLNSPNDVVVKSDGSIWFTDPVFGLLGNY
                     EGYKAEPELDMNVYRIDGATGKASIVAEGVLGPNGLCFSPDEKILYVIESRGVPTRKI
                     LAYDVAASGDKVSNKRVFVDAGPGTPDGMRA"
BASE COUNT          863 a         1468 c         1386 g          879 t
ORIGIN      
        1 tgcttgttgg cgagatagac gttgacgccg ttttccgcga catccaccac gccggtctgc
       61 agcgacgtat agacgctgcc gaacggcatg tgcacgatct gggcgccata ggccggaaac
      121 atggtgtcct cggtgggcgt cgcctgcacc cggaccttga ggcccttcat gtcagcgacg
      181 ttcttgatct cgcgcttgga atacatgttg cgaagcccga gcgtcgacag cgcgatgaca
      241 tgggcgccct gcacggtctc cgcgatcatc gccttgaccg ccttggacac ctcggggtcg
      301 gcgatcgcct tgacgaggtg cgcctccgag cggaacagga agtgcatcga catcacgccg
      361 gcctgcggtg acagggtcgc ggcattggcc gatgacgaaa tgcagaactc cacgtcgccc
      421 gatttcacca gctgcagaac ctgcggctcc tgcccgagct gtgcgcccgg atattgctcg
      481 atcagcatgg tgcccttgct caacgccttc agcttgtcgc tgaagatatc gccgaggatt
      541 ccatagccgg tcgtcttcgg ctgatcgtag gcgaagcgat aatgctttac ttcctgcgcg
      601 ctaacgctag cggccatcac gatcgcggat gcggccacga ccgcgccgag aatgtttttc
      661 aggtttccca tcgtctcccc cttggatgtt ttctttcatc atcccatcgg ggttgtttga
      721 atgtcaatca aacaaaggac gcaaaacgca tgtgcgagat agaaggtagc gcagggaagc
      781 tatacatacc gccgtcatcc tgaggagccg cgcgcttgcg cggcgtctcg aaggatgcgc
      841 cgcttacacc ttcgtcagtc atccttcgag gctcgcccaa gtgggctcgc acctcaggat
      901 gacgacccct tttcccctac cccgcatgaa atcgaaatcg cagccttcgt cggcctgcaa
      961 aatggtttcg ttgaacagat gtgcgtagcc gcgcctggcc cattccggcg tcgcgatcgg
     1021 cgccagcgcc gcgcggcgtt tttcgagctc ggcggcatcg atcaaaagat cgatgcttcg
     1081 tttggcaacg tcgagccgga tcatgtcgcc gttcctcacc agcgccagcg gaccgccgac
     1141 cgcggattcc ggcgtgatgt gcagcacgat ggtgccgaat gccgtgccgc tcatccgcgc
     1201 gtccgatatc cgcaccatgt ccttgacgcc ggtccgtgcg agcttcatcg ggatcggcag
     1261 gtagcccgcc tccggcatgc ccggcgcgcc cttgggaccg gcgttgcgca gcaccagcac
     1321 atcgtcggcg gtcacgtcga gatcggggct atcgactcgc agcgtcatgt cttccaccga
     1381 gtcaaacacc acggcgcggc cggtatgccg cagcaatttc gggctcgccg cggaatgttt
     1441 tatcaccgcg ccgcgtggcg cgagattgcc atgcagcacc gccatcgcgc cttccggctt
     1501 gatcggactg tttcgcgcgc ggatgacatc ctgtcccggc acctcctcgg caccagcgac
     1561 gacatcgcgc aagctcgcgc cggtgatggt tttggcgtcg agatcgatca ggtcgccaag
     1621 ctgcgccatc aatttcggca cccccccggc gtgatggaaa tgctccatgt agtgcgcgcc
     1681 cgacggtttc aagtcgatca ggaccggcac ctcgcggccg agcctgtcaa aagcttcgag
     1741 atcgatgcga tgctccgtgc ggttggcgat cgcggtgaga tgaatgaggc cgttggtcga
     1801 gccaccgatc gcctgcagca ccacctgcgc gttgcggaac gcggccgggg tgaggaactc
     1861 gctcggcttc ggcccctttg ccttcgccat ctcggcggcc accttgccgc tggcttccgc
     1921 ggaacgaaag cgctcggcat gcggcgccgg aattgtcgca ctcatcggca gcgacaggcc
     1981 cagcgactcg gtgatgcagg ccatggtaga ggcggtgccc atcaccatgc aggtgccgac
     2041 cgagggcgcg agccggccat tgaccgcctc gatctcggcc tcgtcgattt cgccggcgcg
     2101 gaattttgcc cacagacggc ggcaatcggt gcaggccccc agcacctcgc ccttgtgatg
     2161 gccgaccacc atcggcccca ccggaataac gacggtcggc agatcggctg agaccgcggc
     2221 catgatctgc gccggcaagg tcttgtcgca accgccgatc acgacgaccg aatccatcgg
     2281 ctgcgcgcgg atcatctcct cggtatccat cgccatcaga ttgcgcagat acatcgaggt
     2341 cgggaacgcg aaactttcgg ctatcgagat ggtcggaaac accatcggca tggcgcccgc
     2401 cagcatcacg ccgcgcttca ccgcctcgat gatctcaggc acattgccgt ggcagggatt
     2461 gtagtcgctg tgggtgttgg tgatgccgac gatcgggcga ttgagcgcat catcggaata
     2521 gcccatcgcc ttgataaagg ccttgcgcag gaacagcgag aatccagcgt cgccataact
     2581 cgtcagtccc ttgcgaagtc cgtcagccat ttgaatttcc tctttgcccc accttacgtc
     2641 ggggcacgcg taacttgaag tcgtcattcc ggggcgggag cgaagcgagc gaacccggaa
     2701 tctcgagatt ccgggttcgc gctagcgcgc gccccggaat gacatcaaag gcgctatttc
     2761 aagtccacga tcctgctcat gtcgccgtcg aactccatgc cgcaaaacgc gccgccctgg
     2821 tagaaatcgc cgaccggatc cggcggcagt ttcgcctgct cggcaaaagc atgctcgcga
     2881 aaatcctccg ccaggccggt cgggcggtag ccgagatcgt aggctcggtg gttgtcccac
     2941 caggcgcgct cgttgtacga cgcgccgtag aaaatctcga aatggatgtc gggatgatcg
     3001 agcccgatcc ggcacagctg taccaggtct tcgggcttga gccagatcga cagccggcga
     3061 tgatccagcg gcttgtcgcc gaaattgccg atccggatgc aggtgacgcc gagcccgtgc
     3121 ttgtcggcat aaagcgcccc caccgcctcg ccaaacacct tgctgacgcc gtagcggctg
     3181 tcgggacgcg ccgtcacgtc ggttccgatg tggtgatggc gcggatagaa gcccaccgca
     3241 tggttcgatg acgcgaacac gacgcgcttg acgccctttt tccgcgcggc ctcgaacagg
     3301 ttgtagccgc cgatgatatt ggactgcagg atgtcatccc agggtccttc gaccgaatag
     3361 ccgccgaaat gcaggatgcc gtcgacgccg tcgcagatcg cctcgacctg cgcgggatcg
     3421 gacaattcgg ccgccctgaa attttcgtga gcgccgagat cggcgggcgc cttcaggtcg
     3481 ctcagcagca aatgcggata gatcggcggc agcaatttgc gcaaacgtgt gcctattccc
     3541 cccgatgcgc cggtcatcaa tatgcgtggc atttcatccc tctgtttcat cgcattgaag
     3601 cgagtatggc gcggtagctc cgatgcggca agcatccgtg tcccggacgc gatgcggtgc
     3661 gtagcaccgc ttcgcagagc cgggacccac gagcgccacc aacctgttgg gccccggctc
     3721 tgcagcgcat cacttcgtgc tgcgcagcgt ccggggcacg gataacgccc ttgattgctt
     3781 gccgccgcgc gaacgcgatg atagcagacc aggacttgtg aggaagcaca gcaggaacga
     3841 gaacagcaaa tgtccgaaac agtatcccat tcctccggct ggcggccggc gacgtattat
     3901 cccgatccgg ctatacacgc gctcgatccg cggttcgaga aatattggct aaaactctcg
     3961 gcggtagaac ggctgacgac gggattgcgc tgggccgagg ggccggtctg gttcggcgac
     4021 ggacgctatt tgctgtgcag cgatattccc aacaaccgca tcatcaagtg ggaagaggaa
     4081 acaggcacgg tcagcatctt tcgaaaaccg tcgaatttcg ccaacggcca tacccgcgac
     4141 cgccagggtc gcctcattac ctgcgagcat ggcggccgcc gcgtcgtcag aaccgaatat
     4201 gacggctcga tcacggtact gatggactct cttgacggca aacggctgaa ctcgccgaac
     4261 gacgttgtgg ttaaatccga cggttcgatc tggtttaccg atccagtgtt cggcctgctc
     4321 ggcaattacg aaggctacaa ggccgagccc gaactcgaca tgaatgtgta ccggatcgat
     4381 ggtgcgaccg gaaaggccag cattgtcgcc gaaggcgtgc ttggtccgaa cgggctgtgc
     4441 ttttcgccgg acgagaaaat tctctacgtc attgaatcgc gcggcgtgcc gacccgcaag
     4501 atccttgcct atgacgtcgc agccagcggc gacaaggttt ccaacaagcg cgtgttcgtc
     4561 gatgccggtc ccggcacgcc cgacggcatg cgtgcc
//
LOCUS       DASWGM010000592         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1159420,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000592 DASWGM010000000
VERSION     DASWGM010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159420"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /gene="rsfS"
                     /locus_tag="VGA15_16835"
     CDS             complement(<1..296)
                     /gene="rsfS"
                     /locus_tag="VGA15_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX9589398.1"
                     /translation="MKAQPDADKTLNMILSRLDDMKAEETVTIDLRGKSAFSDYMIVT
                     SGRANRHVGAIAENVAKGLKETGIKSIHIEGLPNCDWVLIDSGDVIVHVFRPEVR"
     gene            complement(508..1131)
                     /locus_tag="VGA15_16840"
     CDS             complement(508..1131)
                     /locus_tag="VGA15_16840"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX9589399.1"
                     /translation="MPSISQAIPLYTNGMRIGLLGGSFNPPHVAHRAISLFAIKRLKL
                     DRVWWLVTPGNPLKDQGTLHDLEERMAAARKMANDPRIDVSCLESVIRTRYTVDTITY
                     LRRRASGLRFVWIMGADNLAQFHRWQNWRRIASEAPIAVIDRPPQSFRALAAPAAQAL
                     AHYRLPENQAGRLADQRAPAWVFLTGMKLNLSSSGLRNPDGSWRTQK"
     gene            complement(1259..>2206)
                     /locus_tag="VGA15_16845"
     CDS             complement(1259..>2206)
                     /locus_tag="VGA15_16845"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX9589400.1"
                     /translation="PNGMIIERVRVPLGVVAVIFESRPNVAADAGVLCLKSGNAVILR
                     GGSDSFRSCWAIHDCLVRGLREADLPEASITLVPTRDRAAVGLLLSGLNGGVDVIVPR
                     GGKSLVARVEAEARVPVFAHLEGVNHVYVDHAANLEMAKSIVLNAKMRRPGVCGAAET
                     LLVDRKGASTNLKPLVTMLIDAGCEVRGDDAVQRADPRVKPASDEDWGTEYEDAIITA
                     KVVDGVGEAIAHIHNHGSHHTDAIVTEDAGAARKFLNEVDSAIVLHNASTQFADGGEF
                     GFGAEIGIATGKFHARGPVGAEQLTSFKYRVHGTGQTRP"
BASE COUNT          385 a          678 c          704 g          439 t
ORIGIN      
        1 cgcacctcgg gcctgaatac gtgcacgatc acgtccccgg aatcgatcag cacccagtcg
       61 cagttgggta agccctcgat gtggatactt ttgatcccgg tttccttgag acctttggcg
      121 acattttccg cgatcgcgcc gacgtgccgg ttggcccggc ccgaggtgac gatcatgtag
      181 tcggaaaatg cagatttacc gcgaaggtcg atggtgaccg tttcttccgc cttcatatcg
      241 tcgaggcggg agaggatcat attcagggtc ttgtcggcgt cgggttgcgc cttcaaggcc
      301 gcggttggtg tcgatttacg cgccgttttg ggagccttat cagtggcctt ggacaaaaca
      361 gacttggaca atacagatgt ggccagggac cattcctttc actgtaccgc gaacgccgaa
      421 tcctgaggcg ccgctgacta tactacgcat gtggggttaa cggtttcaat attccagtga
      481 gcccatttct cacttcacgc ccgcttgtca cttctgtgtt ctccagctgc cgtccgggtt
      541 ccgcaggccg ctcgatgaca ggttcagctt catgccggtc agaaagaccc aggctggcgc
      601 gcgctggtcg gccagccggc ctgcctggtt ctcgggcagc cgatagtgcg cgagcgcctg
      661 ggctgcaggc gcggccagcg cgcgaaaact ttgcggcggc cggtcgatca cagcgatcgg
      721 cgcctcggat gcgatgcgcc gccagttctg ccagcgatga aattgcgcga gattgtcggc
      781 gcccatgatc cagacgaagc gcaggccgga agcgcggcgg cgcaaataag tgatcgtgtc
      841 gaccgtgtat ctggttcgga tgacggattc gagacagctc acgtcgatgc gcggatcgtt
      901 cgccattttt cgcgcggccg ccatgcgttc ttcgagatcg tgcaaggttc cctggtcctt
      961 gagcggattg cccggcgtca ccagccacca cacgcggtcg agcttcaacc gcttgatcgc
     1021 gaacaggctg atggcgcgat gcgcgacatg gggcggattg aacgaaccgc cgagcaggcc
     1081 gatgcgcatg ccgttggtat agagcgggat cgcctgggag atggatggca acgcgactgg
     1141 agcttgattc aacggagcca ccgccacgtc cgcaatactc ttttcttgtt tgaggcgtct
     1201 tcatcacgcg aaccggatcg cgccctgggt gagctttcgc ttgaaaacgc agtggcgttc
     1261 acggccgggt ctgcccggtg ccatggacgc gatatttgaa gctggtgagc tgctcggcgc
     1321 cgaccgggcc gcgggcgtga aatttgccgg tggcgatgcc gatttccgcg ccgaagccga
     1381 attcgccgcc gtcggcgaac tgggtcgagg cgttatgcag tacgatcgct gaatcgacct
     1441 cgttgaggaa tttgcgcgcg gcgcctgcat cttcggtcac gatcgcatcg gtgtgatgcg
     1501 aaccgtggtt gtgaatatgc gcgatcgcct cgccaacacc atcgaccacc ttcgcggtga
     1561 tgatcgcgtc ctcatattcg gtaccccagt cttcgtcgga agcgggtttt acgcgcggat
     1621 cggcgcgctg cacggcgtcg tcgccgcgca cctcgcagcc ggcatcgatc agcatcgtaa
     1681 ccagcggctt gagattggtc gatgcgccct tgcgatcgac cagcaaggtt tcggcggcgc
     1741 cgcacacgcc gggacggcgc atcttggcgt tcagcacgat cgatttcgcc atttcgaggt
     1801 tggcggcgtg atcgacatag acatggttga cgccttcgag atgcgcgaac accggcacgc
     1861 gcgcttcggc ctcgacgcgt gcgaccagac tcttgccgcc gcgcggcacg atcacgtcga
     1921 cgccgccgtt caatccgctc agcaggaggc cgaccgcggc gcgatcgcgg gtcggcacca
     1981 gcgtgatcga agcctccggc aggtcggctt cgcgcaagcc ccgcaccaga caatcatgga
     2041 tcgcccagca ggagcgaaag ctgtccgagc cgccgcgcag gatcaccgcg ttgccggatt
     2101 tcagacacag cacgccggcg tcggcggcaa cgttggggcg gctttcgaag atcaccgcga
     2161 cgacgccgag cggcacgcgc acgcgctcga tgatcatgcc gttcgg
//
LOCUS       DASWGM010000593         1992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1736035,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000593 DASWGM010000000
VERSION     DASWGM010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736035"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGA15_16850"
     CDS             complement(<1..206)
                     /locus_tag="VGA15_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9589401.1"
                     /translation="MELFTNQVLAGIATGAIYACMALAVVMIYQAIDHLNFAQGEMAM
                     FSTFVCWQLMQWGVPYWGAFLITLV"
     gene            complement(218..931)
                     /locus_tag="VGA15_16855"
     CDS             complement(218..931)
                     /locus_tag="VGA15_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589402.1"
                     /translation="MTAMLNVRDLRAYYGQVQALHGLEFALNEGSLTTLLGANGAGKT
                     TTLRAICNMVRSTGAIEFDGAPLGGRSTENIVRLGIAHVPQGRGTFTTMTVEENLQLG
                     AITRSDKAGVISDIERMYDHFPVLKARHTQQAGTLSGGEQQMLAVARALMLRPRLMLL
                     DEPSFGLAPLIVRDLFKILGKINRDDKVTILVVEQNAQLALELADKAYVIETGRIVMS
                     GSAAEIANNEDVRKSYLGY"
     gene            complement(928..1725)
                     /locus_tag="VGA15_16860"
     CDS             complement(928..1725)
                     /locus_tag="VGA15_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589403.1"
                     /translation="MTQVQLAQGQYPLLAVRDVSVVFGGIIALNGVSFDMHQGQILGL
                     IGPNGAGKTTLFNCLSRLYQPSAGDILMDGQSILRRPPHRIAEIGIGRTFQNVALFPN
                     LSVLDNVRVGTHARTNSDIISDSLKLAWVRRGEAEVNKRVHEILGYLGLDDVAHVTVS
                     GLPFGTQKRVELARALAADPKILLLDEPAGGLNHEEVHVLGDLIRRIRDDRKVTVLLV
                     EHHMGLVMSIADHVVALNFGRKLAEGTPAQVQSDPDVIKAYLGSKDS"
     gene            complement(1795..>1992)
                     /locus_tag="VGA15_16865"
     CDS             complement(1795..>1992)
                     /locus_tag="VGA15_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX9589404.1"
                     /translation="YAFNCGAPAFRFTEDRLRNDIGPRLVAMVRNIEAALGGAAPQSK
                     KEHNKKLKAGGKVARVVEGIR"
BASE COUNT          373 a          616 c          615 g          388 t
ORIGIN      
        1 accagcgtga tcaggaacgc cccccaatag ggcacgcccc attgcatcag ctgccagcaa
       61 acaaaggtcg agaacatcgc catttcgccc tgggcgaaat tgagatgatc gatcgcctgg
      121 tagatcatga cgacggcaag cgccatgcag gcatagatcg cgcctgtggc gatgccggcc
      181 aggacttgat tggtaaaaag ttccatggcc gcgctcctca ataacccaga taggatttac
      241 ggacgtcttc gttgttcgcg atctcggcag cacttcccga catcacgatg cgcccggttt
      301 cgatcacata ggccttgtcg gcgagctcca gcgccagctg cgcattctgc tccaccacca
      361 ggatggtgac cttgtcgtcg cgattgatct tgccgaggat cttgaacagg tcgcgcacga
      421 tcagcggcgc caggccgaac gacggctcgt ccagcagcat cagccgcgga cgaagcatga
      481 gcgcgcgcgc gaccgccagc atctgctgct cgccgcccga aagcgtgccg gcctgctggg
      541 tatgccgcgc tttcagcact ggaaagtgat cgtacatgcg ctcgatgtcg gagatgacgc
      601 cggccttgtc gcttcgcgtg atcgcgccga gctgaagatt ttcttccacc gtcatggtgg
      661 tgaaagtgcc gcggccctgc ggcacatgcg cgatgccgag gcggacgatg ttctcggtcg
      721 agcgtccgcc gagcggggcg ccgtcgaatt cgatcgcacc ggtcgagcgc accatgttgc
      781 agatcgcgcg caacgtggtg gtcttgccgg cgccgttggc gccgagcagc gtggtcaggc
      841 ttccttcatt gagggcgaac tcgagaccgt gaagcgcctg aacctgaccg taataggccc
      901 gaagatccct gacgttgagc atcgcggtca tgaatccttg ctccccagat aggccttgat
      961 gacatccggg tcggactgca cctgggccgg cgttccctcg gcaagcttgc ggccgaaatt
     1021 cagcgcgacg acgtggtcgg cgatcgacat cacgaggccc atgtgatgtt cgaccaggag
     1081 gaccgtcacc ttgcgatcgt cgcgaatgcg gcggatcaga tcgccgagca catggacttc
     1141 ctcgtggttg aggccaccgg cgggctcgtc gagcagcaga atcttcggat ccgcggccag
     1201 cgcgcgcgcc agctccacgc gtttctgggt gccgaacggc aggccggaaa ccgtgacatg
     1261 ggcgacgtca tcaaggccga gatagcccag gatctcatgg acccgcttgt tgacttcggc
     1321 ctcgccgcgg cggacccagg cgagcttcag ggagtcgctg atgatgtcac tgttggtgcg
     1381 ggcatgggtg ccgacccgaa cgttgtcgag caccgaaaga ttgggaaaaa gtgctacgtt
     1441 ctggaaggtg cggccgatgc cgatttcggc gatcctgtgc ggcggccgcc tcaagatgct
     1501 ctggccatcc atcaggatat cgccggcgct cggctggtag agccggctca ggcagttgaa
     1561 cagggtggtc ttgccggcgc cgttcggtcc gatcaggcca aggatctggc cctgatgcat
     1621 gtcgaaggag acgccattca acgcaatgat gccgccgaac acgacgctga cgtcgcgaac
     1681 cgctagcagg gggtattgtc cctgcgcgag ctgtacctgc gtcatctcgt cccgcccaca
     1741 ctcgccgggc cggaatgacc gtgcgaattt gttcgccggt cacaatgatg aaggctacct
     1801 gatcccctcg accacacgtg caactttccc tcctgctttc aactttttgt tatgttcttt
     1861 ttttgattgc ggggccgcgc cacctaaggc cgcctcgatg ttccttacca tcgccacgag
     1921 acgaggtcca atgtcgttgc gcagccgatc ttccgtgaag cggaatgcgg gcgcgccgca
     1981 attaaacgca ta
//
LOCUS       DASWGM010000594         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2147049,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000594 DASWGM010000000
VERSION     DASWGM010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2147049"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VGA15_16870"
     CDS             <1..591
                     /locus_tag="VGA15_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9589405.1"
                     /translation="GFIALYAIINSVAGLIWDFTIKQFPTPFGSSPFLGSQLISTHQA
                     GMIGVTLMLLILLFFFFRFTRMGLAMRAAASLPESARLVGINTSWMIALGWGMAAAIG
                     AIAGMLIAPVVFLEPNMMLGVLIYGFAAAVLGGLTSPLGAVVGGFLVGVFENLVGTYI
                     PGVGNELKLPIALALIVTVLVVKPAGLFGRPIVKRV"
     gene            594..1610
                     /locus_tag="VGA15_16875"
     CDS             594..1610
                     /locus_tag="VGA15_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9589406.1"
                     /translation="MSAIQEVVTEAPAVEAVPKRAMTLGYGTSLVVLGLLIVAPMLVK
                     NFIIFQLTMLLIYALAVLALNILTGGSGQFSLGQSAFYAVGAYTSAILMEHVGINYAL
                     TLPVAGIVCFAFGFLFGQPALRLSGVYLALATFALATAMPQLLKLGYFEHWTGGVQGL
                     VVTKPDAPFGLPMSQDMWLYYFTLVVTIAIYIFSVNLLKSRSGRAFMAIRDNEIAASA
                     MGIDVALYKTLAFGVSAGITGVAGGLGAIAVQFVAPDGYTITLAISLFLGMVVGGVGW
                     LPGSIVGSAFIIFVPNIAEGISKGLSGAVFGVLLFLVIFLVPHGARQVAMVAQQLTGK
                     LRKN"
     gene            1624..>1802
                     /locus_tag="VGA15_16880"
     CDS             1624..>1802
                     /locus_tag="VGA15_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9589407.1"
                     /translation="MLSNQKLRIAALSTAVIAFAATGGTAFAQKKYDTGATDTEIKIG
                     NIMPYSGPASAYGVIG"
BASE COUNT          290 a          543 c          547 g          422 t
ORIGIN      
        1 ggcttcatcg cgttgtacgc gatcatcaac agcgtcgccg gcttgatctg ggacttcacc
       61 atcaagcaat tcccgacgcc gttcggatcg tcgccgtttc tcggcagtca gctcatctcg
      121 acccatcagg ccggcatgat cggggtcacg ctgatgttac tcattctgct tttctttttc
      181 tttcggttta cgcgaatggg cctggcaatg cgcgcggccg cctcgttacc ggaatcggcg
      241 cgattggtcg gcatcaatac cagctggatg atcgcgctcg gctggggcat ggcggccgcg
      301 atcggcgcta ttgccggcat gctgattgcg ccggtggtgt ttttggagcc gaacatgatg
      361 ttaggtgtgc tgatctacgg atttgccgcc gccgtgctcg gcgggctgac cagtccgctc
      421 ggtgcggtgg tcggaggctt cctggtcggc gtcttcgaaa atctcgtcgg gacttacatt
      481 cccggtgtcg gcaatgaact gaaactgccg atcgcgctgg cgctgattgt caccgtcctt
      541 gttgtcaaac ccgccggcct gttcggccgg cccatcgtga agcgagtttg atcatgagcg
      601 caattcagga agtcgtcacg gaagcaccgg cggtcgaggc cgttcccaag agggccatga
      661 cgctcggcta cggcacttcg ctggtggtgc tgggattgct gatcgtagcg cccatgttgg
      721 tgaagaattt tatcatcttc cagctgacga tgctgctgat ctatgccctc gctgttctgg
      781 cgctgaacat tctaaccggc ggcagcggcc agttttcgct gggccagagc gcgttttacg
      841 cggtgggcgc ctacacctcg gcgatcctga tggagcatgt cggcatcaat tacgccctga
      901 cattgcccgt cgccggcatc gtctgctttg cctttggatt cctgttcggc cagccggcgc
      961 tgcgcttgag cggcgtctat ctggcgctgg cgaccttcgc attggcaacc gccatgccgc
     1021 agctcctgaa gctcggctat ttcgagcact ggaccggcgg cgtgcaaggc ctggtggtga
     1081 ccaagcccga tgcgccgttc ggtctgccga tgtcgcagga catgtggctt tattatttca
     1141 cgctcgtggt cacgatcgcg atctatattt tctcggtcaa cctcctgaag tcgcgttcgg
     1201 ggcgcgcctt catggcgatc cgtgacaatg aaatcgcggc gtccgccatg ggcatcgacg
     1261 tcgcgctgta caagacgctg gcgttcggcg tcagcgccgg catcaccggg gttgccggcg
     1321 gcctgggggc gatcgccgtg cagttcgtcg cacccgacgg ttacaccatc acgctggcga
     1381 tctcgctgtt tctcggcatg gtggtcggcg gcgtcggctg gttgcccgga tcgatcgtcg
     1441 gctcggcctt tattattttc gtgccgaaca tcgcggaggg aatttcgaaa ggtctctccg
     1501 gcgcggtgtt cggcgtgctg ctgtttctcg tcatcttcct ggtgccgcac ggcgccaggc
     1561 aagtcgccat ggtcgctcag caactgaccg gcaaactcag gaagaactaa ctaaggagag
     1621 aatatgcttt ctaatcaaaa attacgtatc gccgcgcttt cgacggcggt catcgctttc
     1681 gccgccaccg gcggcactgc atttgcccaa aagaaatacg acaccggcgc caccgacacc
     1741 gagatcaaga tcggcaacat catgccctat agcgggcccg cctccgccta tggcgtcatc
     1801 gg
//
LOCUS       DASWGM010000595         5364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2290247,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000595 DASWGM010000000
VERSION     DASWGM010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2290247"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VGA15_16885"
     CDS             <1..615
                     /locus_tag="VGA15_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9589408.1"
                     /translation="LRLAEERTWERREAFLALNPAGTTPVLISERQPAIPGAGIIAEY
                     LDETHGARMDERRLLPSAMGDRIEVRRLMAWFNEKFFEEVSNPLVTERIYKRFMSEED
                     GGGAPATDVIRAAKANVRYHLAYIGWLAQTRNFLAGDHLTYADLAAAAHLSAIDYLGD
                     VPWIEDDAAKAWYARVKSRPSFRPLLSEWLAGVPASPTYVDLDF"
     gene            788..1903
                     /gene="queG"
                     /locus_tag="VGA15_16890"
     CDS             788..1903
                     /gene="queG"
                     /locus_tag="VGA15_16890"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX9589409.1"
                     /translation="MSSLSIESLKQQALALGFDCVGVADPAAIADAAKYFRSFLESGA
                     HGDMDWLASNPERRADPRMLWPDVRSVIMLGLNYGPDQNPLAILEQRTRGAISVYAQG
                     DDYHDLVKKRLKALARWLVAASGAEVKVFVDTAAVMEKPLAQVAGLGWLGKHTNLVSR
                     EFGSWLFLGAIFTTSDLPRDDTEADHCGSCQACLDICPTAAFPAPYKLDARRCISYLT
                     IENKGPIPREFRKAIGNRIYGCDDCLAVCPWNKFAQTGREAKLAARDELRAASLTDLA
                     RLDDTAFRALFAKSPVKRIGRNRFIRNVLIAIGNSNDSALAIEAERLLDDASPLVRGA
                     AVWALSQLMGQGEFEALAARAISTELDEGVQVEWSRA"
     gene            2128..2925
                     /locus_tag="VGA15_16895"
     CDS             2128..2925
                     /locus_tag="VGA15_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase domain-containing protein"
                     /protein_id="HEX9589410.1"
                     /translation="MTNQPFFTTERDAFIPTKVANGPWDPNSLHGRVIIGLLAFVIEQ
                     RHGSDDFVPARLTVDMFRLPNMAPIEVKTKLVRDGLRIKVVEAEFFSGGSSMARASCQ
                     LLRKTENPPGQVWSPPNWGVPLPADIAAPTDPRLSLNGKWTTRPITGAMGTVGPRRLW
                     MSEVRDLVEGAPLTPFVRVAVAADFASPFANAGDQGLGYINSDVTLYLHRLPVKEWIG
                     FEVVNHHATDGVAIGECFLYDQQGPIGTAIVAALAQRKPMAKPPPPS"
     gene            complement(3066..3851)
                     /locus_tag="VGA15_16900"
     CDS             complement(3066..3851)
                     /locus_tag="VGA15_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9589411.1"
                     /translation="MTNSASGPQPAFLVVGKDGAARSIAVRARPGGAPGLFWLGGFHS
                     DMQGTKAIALDAWAAEHHRACVRFDYSGHGESGGDFIDGTIGRWLEESVAAFEQFCTG
                     PQVVIGSSMGGWMALLLAREIVNRPENRASLAGLVLIAPAPDFTEQLMWNGFSPEIQQ
                     EIQTKGVWLRPSQYGDPYPITRALIEEGRNHLLLGSAINVGCPVRILQGAQDPDVPWR
                     HAFALAHRLPSDDVVLTMIQDGDHRLSRPQDIARIIAAVAEIG"
     gene            4128..4667
                     /gene="infC"
                     /locus_tag="VGA15_16905"
     CDS             4128..4667
                     /gene="infC"
                     /locus_tag="VGA15_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX9589412.1"
                     /translation="MRRPNRAPPAATKDGPRTNDEIRNHEIQLIDQTGLNHGNVETIA
                     AIKMAMEAGMDLVEISPNTNPPVCKIMDYGKFKYSAQKKAAEARKKQKVVEIKEIKLR
                     PMIDDHDYDVKMRAMQRFFEEGDKVKVTLRYRGREMAHQEIGTKLLDKVKSDVAEIAK
                     VEQDARFEGRQVVMVLAPR"
     gene            complement(4751..5191)
                     /locus_tag="VGA15_16910"
     CDS             complement(4751..5191)
                     /locus_tag="VGA15_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589413.1"
                     /translation="MGKKMRISARPMALFAGLWPARALPWIFATAVLAAVAAPSAANA
                     RKAGGAGGGYDGIWSVLIITQAGSCDPAYSYPFRVSGGRISTAGAADVSGSVGPGGVV
                     AVRISAGGRVASGNGRLSGSSGSGRWTAKVSSGNCSGRWQATRG"
BASE COUNT         1020 a         1693 c         1666 g          985 t
ORIGIN      
        1 ctgcggctgg ccgaagagcg gacctgggag cgccgtgagg cgtttcttgc gttgaacccg
       61 gccggcacca cgccggtatt gatatcggaa cgccagccgg cgattcccgg cgccggaatc
      121 atcgccgaat atctcgatga aacccatggc gcgcggatgg atgaacggcg gctgctgcca
      181 tcagcgatgg gggatcgcat cgaggtgcgc cggttgatgg cgtggttcaa cgagaagttt
      241 ttcgaggagg tctccaaccc cctggtcacc gagcgcatct acaagcgttt catgagcgag
      301 gaagatggcg gcggcgcgcc ggcgacggat gtgattcgtg cggccaaggc aaatgtgcgc
      361 tatcatctgg cctatatcgg ctggctggcg cagacgcgga attttctggc cggcgaccat
      421 ctgacctatg cggacctcgc cgccgcggcg cacctttcgg cgatcgatta tctgggcgac
      481 gtgccatgga tcgaggacga cgcggcaaag gcgtggtacg cgcgggtgaa atcccgcccg
      541 tcgttccgcc cgctgctgag cgaatggctg gcgggcgtgc cggcgtcgcc cacctatgtg
      601 gatctcgact tctgaatatg acgggtatcg tgccccggac gctgcgcagc gcgaagcgat
      661 gcgctgctga gccggggccc atcttcttat gtggtaataa cgtgggtccc ggctctgcgg
      721 agcagcgtga agaacgctgc accgcgtccg ggacacgaag aaagatttac gctttaacgg
      781 gtttccagtg agctcgcttt cgatagagtc gctaaagcag caagcgctcg cgctcggttt
      841 cgattgcgtc ggcgtcgccg atccggccgc gattgcggat gccgcaaaat attttcgcag
      901 cttcctcgag tccggcgcgc atggcgacat ggactggctc gctagcaacc ctgaacgccg
      961 cgccgacccg cggatgctgt ggcccgacgt gcgctcggtg atcatgctcg gcctcaatta
     1021 cggaccggat cagaatccgc tggcgatact cgagcagcgc acgcgcggcg cgatttcggt
     1081 ctacgcccag ggcgatgatt atcacgacct ggtcaagaag cggctgaagg cgctggcgcg
     1141 atggctggtc gcggcatccg gcgccgaggt caaggtattc gtcgacaccg ccgctgtgat
     1201 ggaaaaacca ctggcgcagg tggcgggact gggctggctg ggcaagcata ccaacctggt
     1261 gtcgcgcgaa ttcggctcat ggctgtttct cggcgcgatc ttcaccacgt cggaccttcc
     1321 gcgcgacgac accgaggccg accattgcgg ctcatgtcag gcctgcctcg atatctgccc
     1381 gaccgcggcg tttccggcac cttacaagct cgatgccagg cgctgcattt cctatctcac
     1441 catcgaaaac aagggtccga tcccgcggga attccgcaag gcgatcggca accgcatcta
     1501 tggctgcgac gattgcctcg ccgtctgccc ttggaacaag ttcgcgcaaa ccgggcgcga
     1561 agcaaaactc gccgcgcgcg atgaattgcg cgcggcttcg ctgaccgatc tggcgcggct
     1621 cgacgatacg gcgtttcgcg cgctgttcgc aaaatcgccg gtcaagcgca tcggccgcaa
     1681 ccggttcatc cgcaatgtgc tgatcgcgat cggcaactcc aacgacagcg ctttggccat
     1741 cgaagccgaa cgcctgttgg atgacgccag cccgctggtc cggggggccg cggtgtgggc
     1801 gctgtcgcaa ctgatggggc agggggagtt cgaggcgcta gcggcgcgtg cgatcagcac
     1861 cgaattggat gaaggcgtgc aagtggagtg gtctcgcgcc taaatattcg gccgtcttgc
     1921 cccgcgaaag cggcgcattc agtattccag agcggttcga gataaaccga gacatcacgg
     1981 cgtactggat cgtccgctgg agcctgtcat cgggcttgcg aagcgagacc cggtggcgga
     2041 cgatgacggc cggtattgtg ctgaccgtct tttcctcatc gccccgccct tgatttaacg
     2101 ctgcaaattc cgtcatggtc ccgcgccatg acaaatcagc ctttcttcac gacggaacgc
     2161 gacgccttca ttcccaccaa ggttgcgaac ggaccatggg atcccaattc gctgcacggc
     2221 cgtgtcatta tcggcctcct ggccttcgtg atcgaacagc gccacggcag cgatgacttc
     2281 gtgccggcgc ggctgacggt cgatatgttt cgcctgccaa atatggcgcc gatcgaagtc
     2341 aaaactaaac tcgtccgcga tggcctgcgc atcaaggtgg tcgaagccga gtttttcagc
     2401 ggcggcagca gcatggcgcg ggcgtcctgc cagctgctgc gaaagaccga aaacccgccc
     2461 ggccaggtct ggtcgccgcc aaattggggc gtgcctctgc ccgccgacat tgcggcgccg
     2521 accgatccgc gcctcagcct gaacggcaaa tggaccaccc gtccgattac gggtgcgatg
     2581 ggcaccgtcg gcccgcggcg gctgtggatg agcgaagtgc gcgacctcgt cgagggcgcg
     2641 ccgctgacac cgttcgtgcg cgtcgcagtg gcggcagatt ttgccagccc gtttgccaat
     2701 gccggcgatc agggcctcgg ttacatcaac agcgacgtca cgctttatct gcaccgcttg
     2761 ccggtgaaag aatggatcgg cttcgaggtg gtgaaccacc acgccaccga cggcgtcgcg
     2821 atcggcgagt gttttctcta cgaccagcag ggcccgatcg gaaccgccat cgtcgccgcc
     2881 ctggcgcagc gcaagccgat ggcaaagccg ccaccgccgt cgtagccgtc attccggggc
     2941 gacgccaacg ggtccggccg ttggccgacc cgatgacagg cgtcgagccc ggaatctcga
     3001 gattccgggc tcgctcgttt cactcgcgcc ccggaatgac agcgtcatga gaatcgcaaa
     3061 acacctcacc caatctccgc caccgccgcg atgatgcgtg cgatgtcctg cggccgcgac
     3121 aggcggtgat cgccgtcctg gatcatagtc agcaccacgt cgtcactggg cagccgatgc
     3181 gccagcgcga atgcgtgccg ccacggcacg tccggatcct gcgcgccctg caggatccga
     3241 accggacatc ccacgttgat ggcactgccg agcaaaagat ggttgcggcc ttcctcgatc
     3301 agggcgcggg tgatcggata gggatcgccg tattgcgacg gccgcagcca aacacctttg
     3361 gtctggatct cctgctgaat ttcaggcgaa aagccgttcc acatcagctg ttcggtgaaa
     3421 tccggcgccg gggcgatcag caccagcccg gccagtgacg cgcgattttc cggccggttg
     3481 acgatttcgc gggccaatag cagcgccatc cagccgccca tcgatgagcc gatcacgacc
     3541 tgcggccctg tgcaaaactg ctcgaatgcc gcgacgctct cttcgagcca gcgcccgatg
     3601 gtaccgtcga tgaagtcacc gccggattcg ccgtggccgg aatagtcgaa tcgcacgcac
     3661 gcgcgatgat gttcggctgc ccaggcatcg agcgcgatgg ctttggtgcc ctgcatgtcc
     3721 gaatggaagc cgcccagcca gaacagccca ggcgcgccac cgggacgggc ccgcacggcg
     3781 atgcttcgtg cggcgccatc cttgcctacc acgaggaagg cgggctgcgg cccggatgcg
     3841 gaattcgtca tgagtcgtta cccttgtccg ctgctgtttg ggtgagcagg cccaatccgt
     3901 caacgggaag gctgataccc tattcggggg caatcgcgac gcttgcggaa ctagccctcg
     3961 ccctctatgt gggcaggtct gaacaaaacc ttcatgccgg cgttagccag cgtgactaaa
     4021 ttgcctcgta tttgacggtt ttgaagcgca acggtgcgag ttcgcttgcc cgctgggcct
     4081 tattccttca cactagagat atcgtccaca accttggaga gctaaccatt cgccgtccca
     4141 acagagcccc gcccgctgcc acaaaagacg ggccgcgcac caacgatgaa atccgcaatc
     4201 acgaaatcca gctgatcgat cagacaggcc tgaaccacgg caatgtagaa acgatcgccg
     4261 ccatcaagat ggcgatggaa gccggcatgg accttgtcga gatttcaccc aataccaatc
     4321 ctcccgtttg caagattatg gattacggga agttcaaata ttcggcgcag aaaaaagccg
     4381 ccgaagcccg caagaagcag aaggtcgtcg agatcaagga gatcaagctc cgaccgatga
     4441 tcgacgatca cgattacgac gtgaagatgc gtgcgatgca gcggttcttc gaggaaggcg
     4501 acaaggtcaa ggtcaccttg cgctatcgcg gccgcgaaat ggcccatcag gagatcggca
     4561 ccaagctttt ggacaaggtc aagagcgacg tcgctgaaat cgcgaaggtc gagcaggacg
     4621 cgcgtttcga aggccgtcag gtggtgatgg ttctggcacc acgctagctc gcaacggctg
     4681 ttcgcgtttg tgaattttgg attacggccc gtcaggcaat gtggcgggcc attttctttc
     4741 caagagatga tcagccccgc gtcgcctgcc agcggccgct gcagtttccg ctcgacactt
     4801 tggccgtcca gcggcccgag ccgctgctac cgctgagcct gccgttgccc gacgccaccc
     4861 ggccgccggc cgaaatcctc acggcgacaa cgccacccgg tccgacactt ccagacacat
     4921 cggctgcccc cgcggtcgaa atgcggccgc ccgagacccg gaacggatag ctgtaggcgg
     4981 gatcgcaact gccggcctgc gtgatgatca ggacgctcca gattccatca tagccgccgc
     5041 cggccccgcc ggctttccta gcattggcgg cgctgggcgc ggccaccgcg gcgagaacgg
     5101 cagtggcaaa tatccatggc aaggcgcgcg cgggccaaag accggcaaaa agcgccatcg
     5161 gtcgggctga tatcctcatt ttctttccca tttttggccg ccgcatgggc ccgtgagccg
     5221 atacggcgaa tcaaagggtt ccacggttca tcgttgccaa tctggcttgg ctctgccata
     5281 agcgcaaact tcatcgcccg gctgatcaag ggctgccgtg tcgatgcccg tgctggttgt
     5341 tttgtttgtg gaatagccga gcac
//
LOCUS       DASWGM010000596         6185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_74400,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000596 DASWGM010000000
VERSION     DASWGM010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74400"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VGA15_16915"
     CDS             <1..508
                     /locus_tag="VGA15_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589414.1"
                     /translation="SELAGCNAKFAGRRKPSGGYTYYDFMQNRSFDIAGPNPTPEEQK
                     YIDQQYTVFLDKQRRSYIAAALTAKQDEQNQQNQQQQASLRNEKASMPMPPETPVKPK
                     ASAADLKSRAKANCAKHSTFSCEWPVLSEKITNLKKALFGSSQAKVKHGGSSTRGEAS
                     EPVQSAAR"
     gene            complement(1161..2162)
                     /locus_tag="VGA15_16920"
     CDS             complement(1161..2162)
                     /locus_tag="VGA15_16920"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9589415.1"
                     /translation="MISNRPIGVAGAGSIGCFVGGMYAVSGRRVALLARPRVIEEIEG
                     NGLRLTGFGNFERNIAADALTLSEDSRIFADAGTVLVTVKSADTAEMADIIATHAPAD
                     AVIVSLQNGVGNVAVLREKLPGRRVLAAMVPFNVIALGDGRFHRATSGDIIIERDDGG
                     TAQQLSVPGLRMLATDDIAGVQWGKLLVNLFNALNALADVPLRQQLAQRSWRRLFADQ
                     MAEGLSAIRAAGIKPVSPTPVPASLTPPLLRLPDAMFGILLGRTMKIDPEARSSMWED
                     LQRGRRTEIDYLQGVITGIADRYGLQVPLSRRVVALIKSAEASGKGSPGLTPEQIRG"
     gene            2318..2893
                     /locus_tag="VGA15_16925"
     CDS             2318..2893
                     /locus_tag="VGA15_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEX9589416.1"
                     /translation="MGLLDVLNGMQQGPRGPSNPSAQSSGGMSPMTMAILGLLAWKAV
                     KHLSGSQPGATPAPAPSPNTVNAGLPGGGMGGGGMGDLLKGGLGGLLAGGAAGSVISG
                     GLGDLLKQFQQNGQGETANSWVSPGPNKQISPRDLASALGADQINSLISQSGLSRDEL
                     LAGLSQHLPDVINHLTPDGRLPTENELSGRL"
     gene            2975..3253
                     /locus_tag="VGA15_16930"
     CDS             2975..3253
                     /locus_tag="VGA15_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX9589417.1"
                     /translation="MSGILWVIIVGFVAGIIARLVSPGPNNPSGFILTTVLGIAGAFL
                     ATFIGQAIGHYDLDQGAGFITATIGALVVLFIWNRLVASRVISDPGNR"
     gene            complement(3395..4177)
                     /locus_tag="VGA15_16935"
     CDS             complement(3395..4177)
                     /locus_tag="VGA15_16935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589418.1"
                     /translation="MSKDGLCVIVTGSASGLGAATAAIFAKGGARIVVNYSSSQKEAE
                     QTADLCRGAGGEVVVVQGDVSRDEDCKKIVAAAAPWGRLDALINNAGTTKHMAHENLD
                     GLSAEDFQRLFGVNTIGPFQMVRASRSLLEAGAAASGRASAVVNVSSVAGISGIGSSI
                     AYAASKGALNTITYSLARALAPLIRVNTVCPGYIDTPWFTKGRGAEGAARVRDAVVAK
                     VPLKRASTAEDVAELVCFLATPQSSNMTGEVVRMDAGAHLIT"
     gene            4453..5517
                     /locus_tag="VGA15_16940"
     CDS             4453..5517
                     /locus_tag="VGA15_16940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9589419.1"
                     /translation="MAESENIVAETAEKIFSDLADAQTINRDQKGDWKAPLWRALTEA
                     GLPLSWVPEDCDGSGASLAEGFSVLSAAGRFAIAVPLAETMLAGWLLSQSKIASPLGM
                     MTIAPASPKDRIAQNADGTLSGRARGVPFAKDAKHIAVLAHGAGDLSIALVEVSKCRI
                     EAGLNLASDGSDTVTFNNVPPVAIKPAPKGFDQTSLMLMGAVVRSLQIAGALESMLDV
                     SVRYSNERVAFEKKISKFQAVQHNLARLAGETAAAMTAASSAADTIANATGFDDAVFL
                     EATSAKIRCAEAAEKGAAIAHQVHGAIGYTLEHILHRYSMRALAWRDDFGPESYWAVE
                     LGKRVAARGADELWPLVASR"
     gene            5800..>6185
                     /locus_tag="VGA15_16945"
     CDS             5800..>6185
                     /locus_tag="VGA15_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9589420.1"
                     /translation="MTAALRFDPIRLPPECEKLRKEVRSFLADEIAAGTFDPHSPQGG
                     DNDVPGFSKRVGERGWIGMTWPKKYGGHERSFLERYVVTEEMRVANAPTRRFFVADRQ
                     SGPVLLKYAPEHIKMDILPRICRGEV"
BASE COUNT         1204 a         2007 c         1893 g         1081 t
ORIGIN      
        1 gtcggagctc gccggctgca acgcgaaatt cgccggacgg cgcaagccca gcggcggcta
       61 cacctattac gacttcatgc agaatcgcag cttcgatatc gccggcccca acccgacgcc
      121 ggaagaacag aaatatatcg atcagcaata caccgtcttt ctcgacaagc agcggcgaag
      181 ctacattgcg gcggcgctca cggccaagca ggacgagcag aaccagcaga accagcagca
      241 gcaagcctcg ctgcggaacg aaaaggcgtc catgccgatg ccgccggaga cacccgtcaa
      301 gccaaaggcc tcagcagccg atctgaagtc gcgcgcgaaa gcgaattgcg ccaaacattc
      361 caccttctcc tgcgaatggc cggtgctgtc cgagaaaatc accaatctga aaaaagcgct
      421 gtttggctct tcccaggcca aagtcaaaca cggcggatcg tcaacccggg gcgaggccag
      481 cgagcccgtg caaagcgcgg cccgctgaca ggctccgcct tgatgcctaa tccagagatg
      541 atcggcgcga gatcgggatt ttcgggttcg cgcggagccc gtcatcggga ggcgcagcgc
      601 cccgacccgt tggcgcgccc cggaatgacg ctatcgtgtc agtccgtcat caccgtcgcc
      661 cagaattttc ggtaaggcga tttgcgcaag cccgctaccc ctgcgtttca atgaacgcct
      721 ccgtatggtg gcttcacatg gcgccggctg gcatggtgcc gggaaatcgg tacgggaggc
      781 cgtatcatcg gttcataaat ctcccgcgcc gctgcggctc gctgctcctg caacctcgca
      841 tcatatcccg gtgacggcgt gacggtcggc ggcgcggcac tcgctggcat caccaacgca
      901 acgccgcaga gcgcgacagc ggcggcgatg gcaccatttg cgtaaaatcg cttcatgtca
      961 ttcctcccgg cgctgacgcg gcggatgtcc gcatcgccgc tgacgcgtca ttcgcgcttg
     1021 atcgtcgcga tttcaaggca agttagcgtg accgacgatt agagcatgat ccggaagagt
     1081 ggaaaccggt tttccgataa agatcatgct cgaacaaaga gatgagatca tgatccgatt
     1141 caatctaatc ggatcatgat ctagccgcgg atctgctccg gcgtcagccc gggggaacct
     1201 ttgccgctcg cctcggcgct tttgatcagc gccacgaccc ggcgcgacaa cggcacttgc
     1261 agcccgtatc gatcggcgat gccggtgatg acgccttgca gataatcgat ttcggtgcgc
     1321 cgtccgcgct gcaaatcctc ccacatcgat gagcgcgcct cgggatcgat cttcatggtt
     1381 cgccccagca atatcccgaa catcgcgtcc ggcagccgca acaagggcgg tgtcaaactc
     1441 gccggaaccg gcgtcggcga caccggtttg atgcccgccg ccctgatcgc cgaaagaccc
     1501 tcggccatct ggtccgcgaa cagccggcgc catgagcgct gcgcaagttg ctggcgcagc
     1561 ggcacatcgg ccagcgcgtt gagcgcattg aagagattga ccagaagctt gccccactgc
     1621 acgccggcga tgtcgtcggt cgcaagcatc cgcaaccccg gcaccgagag ctgctgcgcg
     1681 gtgccgccgt cgtcgcgttc gatgatgata tcgcctgagg tggcgcgatg aaatcgacca
     1741 tcgccaagcg cgatcacatt gaacggcacc atcgccgcca gcacgcgccg ccccggcaat
     1801 ttctcgcgca acaccgcgac attgccgaca ccgttctgta ggctgacgat gacggcatcg
     1861 gcgggagcgt gcgtcgcaat aatatcggcc atctcggcgg tatccgcgct tttcacggtc
     1921 accagcacgg ttccggcatc ggcaaaaatc cgggaatctt cggagagcgt cagcgcgtcc
     1981 gcggcgatgt tccgctcgaa gttcccaaaa cccgtcagcc gcagcccgtt accttcgatt
     2041 tcctcgatca cgcgcgggcg cgccagcaag gcgacacggc ggccggagac cgcgtacatg
     2101 ccgccgacaa agcagccgat gctgccggcc cccgccacgc cgatcggtcg gttcgaaatc
     2161 atcttgcctt ccagcaatgt cgcctgggtt cgatagcaga gtttcgtgct ccacgcctgt
     2221 tcggagagcc gcgtgcttgg aatagccgcg ccttgtaacg gtcatggcgc accgttatgt
     2281 ttcgtggggt ggggctggca ctcggcagga gagaacaatg ggtttactcg acgtactcaa
     2341 cggcatgcag caaggcccgc gcggcccgag caatcccagc gcgcaaagca gcggagggat
     2401 gtcgccgatg accatggcga tccttggact gctcgcctgg aaggcagtca aacatctcag
     2461 cggcagccag ccgggcgcga ccccggcgcc ggccccatcg cccaataccg tgaatgccgg
     2521 cctgcccggc ggcggaatgg gtggcggtgg tatgggcgat cttctcaagg gtgggttggg
     2581 cggcctcctt gctggcggag ccgctgggag cgtgatcagc ggcgggctcg gcgacctgct
     2641 caagcaattt cagcagaacg gccagggcga gaccgccaac tcatgggtga gtccgggccc
     2701 gaacaagcag atttcgccgc gcgatctcgc cagcgccttg ggcgccgatc aaatcaatag
     2761 cctgatatcg cagagcggtc tgtcgcgcga cgaactgctc gccggcctca gccagcattt
     2821 gcccgacgtc atcaaccatt tgacgccgga cggacggctg ccgaccgaaa acgaattatc
     2881 tggccgactt tgatctcggc cggcaccgcg gttcccttcg cttcgcacag cacagctttt
     2941 tgagattctg cttttcagaa gaaggacgac agccatgagc ggcattctct gggttattat
     3001 cgtcggcttc gtcgcgggaa ttatcgcgcg actggtttcg ccgggcccga acaacccgag
     3061 cggattcatc ctgaccaccg tgctcggcat cgccggcgcg tttctcgcca ccttcatcgg
     3121 ccaggccatc ggtcactacg acctggatca gggcgccggc ttcatcaccg cgacgatcgg
     3181 cgcgctggtg gtgctgttca tctggaacag gctggtggca agccgggtga tttccgatcc
     3241 gggcaacagg taagaggcga agagcggcgc acttgtagcc cggatgagcc aacgggtcgg
     3301 cgcagagcgc cgcccgatga caggctccgc gaaatccggg gccggcatca cgttcgtccc
     3361 ggatttcgct gcgcttcatc cgggctacgg cttctcacgt aatcagatgc gcgccggcat
     3421 ccatccgaac cacttcgccg gtcatgttgc tcgattgcgg cgtggcgagg aagcagacca
     3481 gttcggcgac atcttccgcg gttgaagcgc gtttgagcgg caccttcgca accacggcat
     3541 cgcggaccct ggccgcgccc tcggcgccgc ggcccttggt gaaccacggc gtatcgatat
     3601 agccgggaca gacggtgttg acgcggatca gcggcgccag cgcgcgggcc agtgaatagg
     3661 tgatggtatt caaggcgccc ttgctcgcgg cataggcgat cgacgaaccg atgccgctga
     3721 tgcctgcgac cgacgacacg ttgacgaccg ccgacgcgcg ccccgatgcc gcggcgccgg
     3781 cttccagcag gcttcgcgac gcgcgcacca tctggaacgg gccgatggtg ttgacgccga
     3841 acagccgctg gaaatcctcc gccgacaatc cgtccagatt ttcgtgcgcc atgtgcttgg
     3901 tggtgccggc attgttgatc agcgcatcga ggcgtcccca gggtgccgcg gccgcgacga
     3961 tcttcttgca atcctcgtcg cgtgacacat cgccctggac cacgaccact tcgccacccg
     4021 cgccgcggca gagatcggcg gtctgttcgg cttccttctg gctggaggaa taattaacga
     4081 cgatgcgcgc gccgcctttg gcaaaaatcg ccgctgtcgc agcgcccagg ccggacgccg
     4141 aacccgtgac aatcacgcac aaaccatcct tcgacatccg cgtttcctct tgtcgatttt
     4201 aagcgcccgg tggcgcgcca ccataccgtg caggcaaacg cctgcaaatc cgccaaatct
     4261 ccgttgtgcg gaattaatta ttccgcgcct tgcgttcatg tcgcccttgc agactgccct
     4321 gcggagacag gcgcttgtca tggccatggc ttttcctcat catccggtgc aagaaaagca
     4381 agaaaaggcc gcatcggacg ctggagcgga atggcccggc gagacatgta ccaaatgaaa
     4441 acggggaacg ctgtggcgga gagtgagaac attgtcgccg aaaccgcgga aaagatattt
     4501 tccgatctcg ccgacgcgca gaccatcaac cgcgaccaga aaggcgactg gaaggcaccg
     4561 ctctggcggg cgctgaccga ggccggcctg ccgctgtcgt gggtgcctga ggattgcgac
     4621 ggctcgggcg caagcctcgc cgaaggtttt agcgtgctga gcgctgccgg gcgctttgcg
     4681 atcgcggtgc cgctggccga gaccatgctg gcgggatggc tgctgtcgca gtcgaaaatt
     4741 gcttcgccct tgggcatgat gacgatcgcg ccggcaagcc cgaaagaccg catcgcgcaa
     4801 aacgccgacg gcacgctgtc cgggcgcgcg cgcggcgttc cctttgccaa ggacgccaaa
     4861 catatcgcgg tgctcgcgca cggcgccggc gatctctcga tcgcactggt tgaagtgtca
     4921 aaatgccgga tcgaggccgg cctcaatctc gccagcgacg gcagcgacac cgtgacgttc
     4981 aacaatgtgc cgccggtcgc gatcaagccg gcaccgaaag gttttgatca gacgtcgctg
     5041 atgctgatgg gcgcggtggt gcggagcctg cagattgccg gcgcccttga atcgatgctc
     5101 gacgtcagcg tgcgctactc caacgagcgc gtcgcgttcg aaaagaagat ctcgaagttc
     5161 caggcggtgc agcacaatct cgcgcggctc gccggcgaga ccgcggcggc aatgaccgcg
     5221 gcgagttcgg cggcggacac catcgccaac gccacgggtt tcgatgacgc ggtttttctc
     5281 gaagccacct ctgcaaaaat ccgttgcgcc gaagccgctg aaaagggggc cgcgatcgcc
     5341 catcaggttc atggcgcgat cggctacacg ctcgaacaca tcctgcatcg ctactccatg
     5401 cgcgcgctgg cctggcgcga cgatttcggc cctgaaagct actgggcggt cgaactcggc
     5461 aagcgcgtcg ccgcgcgcgg cgccgacgaa ttatggccgc tggtcgcgtc gcgctagggc
     5521 aatatccatg aatataaaat caatgcgcgc gagttcatct acctctccca tcgggagagg
     5581 tcggcgcgta gcgccgggtg aggggttagg gcctcacgat agaccgtacc cgctcacccc
     5641 cgccctctcc cgttcgaagt cggatatatc cgacttcgac aattcaagat acccaactcg
     5701 ggtaaacccg agttgggtgg agagggggcg cgttcccgct gacgcgagac tcaggcccat
     5761 cactttacaa acataacccc atccgggacg atatttaaca tgaccgcagc tctccgtttc
     5821 gaccccatcc gcctgccacc cgaatgcgaa aagctgcgca aggaagtccg cagctttctc
     5881 gccgatgaaa ttgccgcggg caccttcgac ccgcacagcc cgcaaggcgg cgacaacgac
     5941 gttcccggtt tttccaagcg cgtcggcgaa cgcggctgga tcggcatgac ctggccgaag
     6001 aaatatggcg gacacgagcg cagctttctg gagcgctatg tggtgaccga ggaaatgcgc
     6061 gtcgccaacg cgccgacgcg ccgcttcttc gtcgccgacc ggcagagcgg cccggtattg
     6121 ctgaaatacg cgcccgagca catcaagatg gacatcctgc cgcggatctg ccgcggcgag
     6181 gtgtg
//
LOCUS       DASWGM010000597         7582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2290709,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000597 DASWGM010000000
VERSION     DASWGM010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2290709"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VGA15_16950"
     CDS             <1..266
                     /locus_tag="VGA15_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589421.1"
                     /translation="ANPPEQPTIEELRKRYGTTNDDELILRALVPEADLDRMWAAGPV
                     KTTYPLLSSSELDQVRRLMRTARVPVVQIKSSAMNLSLRRNRA"
     gene            270..1451
                     /locus_tag="VGA15_16955"
     CDS             270..1451
                     /locus_tag="VGA15_16955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX9589422.1"
                     /translation="MRRAVIIDLVRTPFGRARETGALAACHPVDLYAYALKALVRRTR
                     IDPALVEDVITGCVIQVGEQAANIGRQAVLAAGFPESVPAVTLDRKCGSAQQAMDFAA
                     QGVIAGAYDLVIAGGVEMMGLVPMRANRLGKDNLGPLLRQRYPDGFVHQGISAELIAA
                     RWNIPRKSQDMFAERSHRLAAQAEDAGSTAHDIAPVETPTGLVSRDEGLRRDTSVSKL
                     GALKASFHDPEMATRYPQLRWSVTAGNSSQVSDGAGAVLIAEQKTARVLGLTPRAAVT
                     HFAVSGDDPILMLTGVIPATKKLLGRAGIGIGDIDAFEVNEAFAPVVLAWQKEFRPDP
                     ERVNMFGGAIALGHPVGASGVRLTGNLLRALEKTGGRYGLQTMCESGGMANATLVQRL
                     S"
     gene            1477..1785
                     /locus_tag="VGA15_16960"
     CDS             1477..1785
                     /locus_tag="VGA15_16960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEX9589423.1"
                     /translation="MIVVSVLYPSDLESKFDLAYYQNSHLPLCRDLPAPMGMQSLTFY
                     RPIVTDPGAAFQLVAELRFTDLKTTNTALAAHGPRTQADIPNFTDVKPIILIGEEIAG
                     "
     gene            complement(1827..2609)
                     /locus_tag="VGA15_16965"
     CDS             complement(1827..2609)
                     /locus_tag="VGA15_16965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589424.1"
                     /translation="MGRLEGKIALITGAGSGVGRAAMQIFSKEGAKVVGVSRTQSALD
                     ETLKLVKAAGGKGAVVSADLASEAGAAKTMKATLDAFGRIDILVNSAGVGYSWMEKSE
                     GSMNDIATTSLDKWNEVIGINLNSCFLMSKLAVLEMKKHGGGAIVNVTSISGFQGLGV
                     AHSYCAAKGGTINLTRAMCVAYAADNIRTNCIAPGFINTPMVASVINLFDDPNMADRL
                     TPMRRPGTPEEMAYGCLYLASDEASYCNGTVLVIDGGTTARQ"
     gene            2731..3768
                     /locus_tag="VGA15_16970"
     CDS             2731..3768
                     /locus_tag="VGA15_16970"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9589425.1"
                     /translation="MKAAVFRGLGNPLAIETVVDPEIRDGEVLLKVAYCGICGSDLHA
                     TAEGVFVVPDGTVLGHEFSGEVVESADAAWTKGDRLTSVPVNACADEICQRLGACKND
                     LGILCPNNRITGLAVDVPGAYAEYVKVGARQALRLPDGVSYEEGALVEPLAVGLHAVR
                     KAKLGVGARVLIIGAGPIGLSVAAFARLAGAREVVVSEMNATRREKSMAMGATAVIDP
                     ASADTAEAFARIAGCPPDAIFECVGVRGLIQQCIDLSRPRGQIVVVGVCMMEDTVVPI
                     SAILKELDIQFVLGYLSEDFDIVLDAIAKKTINAMPLVSDIVGFEELPAAFEALRRPK
                     GQIKVLIRPGV"
     gene            complement(3885..4619)
                     /locus_tag="VGA15_16975"
     CDS             complement(3885..4619)
                     /locus_tag="VGA15_16975"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9589426.1"
                     /translation="MAESLGLIVPTRGVTDAVDTRSEGERASFPDIVVNAIIRGVHAG
                     RLVPGQRLVEADLTRNLGVSRGPVREALKRLAAEGVVTLNRHRGAFVRAMTRDEVRDT
                     LMILEVLTGLVARLAAQHIDLKGNRETFTAEHEKLMTFRDQGDSIAFLDQRRSYYDTL
                     LRIGGNGELKRLMPLMQIHLLRLQFQPYVTARQREKQFQEYEAISRAVLSGNPRLSER
                     VMHLHIRRTRLALDRLSQEAFAPVQS"
     gene            4957..5415
                     /locus_tag="VGA15_16980"
     CDS             4957..5415
                     /locus_tag="VGA15_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9589427.1"
                     /translation="MLALFALALGTLSARAGDVYVIDNAAGKTPVAIKKDAFALTAVP
                     AMGEGKPNEGFAYTTALELAGTGVQIARGRVEPGGTVATHDGRQQYILYVIGGTGTLG
                     LVDQTGATIGEIKYKPDDVIVFQPNTLHNWKNASAAPFEFLGVDLAPPRK"
     gene            5555..6784
                     /locus_tag="VGA15_16985"
     CDS             5555..6784
                     /locus_tag="VGA15_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9589428.1"
                     /translation="MLYSESNKVIELKRRLQSFMDRHVYPNEERFYQQAEELGPWKVY
                     PVVEELKPKARAEGLWNLFLPESEHGAGLTNLEYAPLCEMMGRSHLAPEVFNCSAPDT
                     GNMEVLARYGTKEHQQRWLKLLLAGEIRSCFAMTEPAVASSDATNIESSIVREGDHYV
                     VNGRKWYTTNATDPRCKICIFMGKTDPDNPDRHRQQSMILVPMDTPGVEVLRPIPVFG
                     FYGVPDRASEVVFNNVRVPATNILLGEGRGFEIAQGRLGPGRIHHCMRLIGLAERTLE
                     KMCVRTRNRVAFGKPVSEQTVTQERIAESRIMIEQARLLTLNAAHMMDTVGNRTAKAE
                     IAMIKVAVPNMACQVIDWAIQAHGGGGTSNDFGLAAAYATARLLRLADGPDEVHRNQI
                     ARLELRKHNITQGGSSD"
     gene            complement(6786..7169)
                     /locus_tag="VGA15_16990"
     CDS             complement(6786..7169)
                     /locus_tag="VGA15_16990"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589429.1"
                     /translation="MTGDLGAIDEDGLLTLKGRAKELIISGGSNIYPREVEEVLLRHP
                     AVAEVAVVGTPEPVWGEKIVAFVVAQTGSQVTASELDTHCLSEIARFKRPREYRFPSS
                     LPKSKYGKVLKRELQAELISTSKAR"
     gene            7294..>7582
                     /locus_tag="VGA15_16995"
     CDS             7294..>7582
                     /locus_tag="VGA15_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589430.1"
                     /translation="MIALIWFVLAVLASPFKSKSRLEAENAVLRHQLIVLRRKVRGRA
                     QLTNNHRWFLVQMYRWFPSILTVVTIVQPETLVRWHRAGFRRYWRWKSNPRG"
BASE COUNT         1466 a         2264 c         2319 g         1533 t
ORIGIN      
        1 tggctaatcc gcccgagcaa ccgaccatcg aggaattgcg caagcgttac ggtacgacca
       61 acgacgatga gctgatcctc cgcgcgttag tgcctgaagc cgatctcgat cgcatgtggg
      121 cggcgggtcc ggtaaagacg acctatccgt tgctgtcatc cagcgaactc gatcaggtcc
      181 ggcggctgat gcggaccgcg cgcgtgccag tggtccagat caagtcatcg gcgatgaact
      241 tgtccctccg gcgaaatcgg gcctgagcgg tgcgccgcgc tgtcatcatc gatcttgttc
      301 gcacgccgtt tgggcgcgcg cgcgagaccg gcgcgcttgc cgcctgccac ccggtcgatc
      361 tctatgcgta tgcgctaaag gctttggtca ggcggacgag gatcgacccg gccctggtcg
      421 aggacgtgat cacggggtgc gtcatccagg tcggcgagca ggccgccaat atcggaaggc
      481 aggcagttct cgcggccggc tttccggaat ccgttcccgc cgtaacgctg gaccgcaaat
      541 gcggctcggc ccagcaggcg atggattttg ccgcacaggg tgtgatagcc ggcgcctatg
      601 atctggtgat cgccggcggc gtcgagatga tgggcctcgt gccgatgcgc gcgaaccggc
      661 tcggcaagga caatctcgga ccgttgctca ggcagcgcta tccggacggt tttgtccatc
      721 agggcatctc ggctgagctg atcgccgcgc gctggaatat cccccgcaaa tcacaggaca
      781 tgtttgctga acgctcgcac cgtcttgcag cacaggccga ggatgcgggc tcgaccgcgc
      841 acgatatcgc gccagtcgaa acgccgaccg ggctggtgtc tcgcgacgag ggtctgcgcc
      901 gcgacaccag cgttagcaag ctcggggcgt tgaaagcatc gttccacgat cccgagatgg
      961 cgacgcgcta tccccagctt cgctggagcg tgacggcggg gaattcgagc caggtcagcg
     1021 acggcgcggg cgccgtgctg atcgccgaac agaagactgc ccgtgtcctc ggcctgacgc
     1081 cacgcgccgc agtcacgcat ttcgccgtat ctggagacga cccgatcttg atgctgaccg
     1141 gtgtcattcc ggcaaccaag aagctgcttg gcagagccgg tatcggcatt ggcgacatcg
     1201 acgccttcga ggtcaatgag gcgttcgcgc ctgtggtgct ggcttggcag aaggagttca
     1261 ggccagatcc cgaacgggtg aacatgttcg gcggcgctat cgcgctcgga catcccgtcg
     1321 gagcgtccgg cgtccggctg accggcaacc tgctgcgtgc tctggaaaag acgggcggac
     1381 gatacgggtt gcagacgatg tgcgagtccg gcggcatggc caacgccacg ctggtccagc
     1441 gtctatcata ggaaccattg aacggagggc aggtccatga tcgtcgttag cgtgctttat
     1501 cccagcgatc tggaatcgaa attcgacctc gcctactacc agaacagcca tctgccgctt
     1561 tgccgcgacc tgccggcgcc gatgggcatg caatcgctga ccttctaccg gccgatcgtc
     1621 accgatcccg gcgcggcctt tcagcttgtc gccgaactgc gtttcacgga tttgaagacg
     1681 acgaacaccg cgctcgccgc ccacggtcct cgcacccaag ccgacatccc gaatttcacc
     1741 gacgtcaagc cgatcatcct gatcggcgag gaaatcgccg gataaacgca ggtcggatcg
     1801 ctccgacccg cgttgatcta ttccgcctac tgcctcgccg tcgtaccgcc atcgatcaca
     1861 agcacggttc cattgcaata gctcgcctca tcggacgcga gatagaggca gccataggcc
     1921 atttcttccg gtgtccccgg acgccgcatc ggcgtcaggc ggtctgccat attgggatcg
     1981 tcaaacaagt ttatcaccga ggcgaccatc ggcgtgttga tgaagcccgg cgcgatgcag
     2041 ttggtgcgga tattgtccgc ggcataggcc acgcacatcg cgcgggtcag gttgatcgtg
     2101 ccgcccttgg ccgcgcagta ggaatgtgcc acgccaaggc cctgaaagcc cgaaatgctg
     2161 gtgacgttga cgatcgcgcc gccgccgtgc tttttcattt ccagcacggc gagtttcgac
     2221 atcaggaagc agctgttcag attgatgcca atgacttcgt tccacttgtc gagcgacgtc
     2281 gttgcaatgt cgttcatcga gccttcgctc ttttccatcc agctgtagcc gacgccggcg
     2341 gagttcacga gaatgtcgat gcgcccgaag gcatcaaggg tggctttcat cgtcttggcc
     2401 gctccggctt cgctggccaa gtcggccgat acgaccgcgc ccttgccgcc ggctgccttg
     2461 accagcttca acgtttcgtc gagtgcgctc tgggtgcgcg agaccccgac gaccttcgca
     2521 ccttccttcg aaaatatctg catcgctgcg cggccgacgc cagaaccggc gcctgttatc
     2581 agtgcgatct ttccttccag ccgtcccata ggttcctccc ggtttgcgga cgtgaaaatg
     2641 ctcgatagtt cgagtttttg tggttgatat caggctagat cgtatgacaa tattgcacaa
     2701 caatttttgt cactctggga ggaaaaagtc atgaaagccg ccgtgtttcg cgggctcgga
     2761 aatccgctcg ccattgaaac ggtcgtcgat ccggaaatcc gggatggcga agttctgttg
     2821 aaggtcgctt attgtggcat ctgcgggtca gatctgcatg cgaccgcaga aggcgtgttc
     2881 gtcgttccgg atggcacggt gctcggtcat gaattctcgg gcgaggttgt ggaaagcgcc
     2941 gacgctgcct ggacgaaggg cgaccggctg acctctgttc ctgtcaatgc ctgtgccgac
     3001 gagatttgcc agcggctcgg tgcttgcaag aacgacctcg gcattctctg tcccaacaat
     3061 cgcattaccg gtctcgcagt cgatgtgccg ggagcctatg ccgaatatgt caaggtcggc
     3121 gcgcggcagg cgctccggct gccagacggg gtgtcctatg aagagggtgc gttggtcgaa
     3181 ccgctcgccg tcggcctcca tgccgtgcgc aaggccaagc tcggcgtcgg cgcgcgcgtt
     3241 ctgatcatcg gcgccgggcc gatcggcctg tcggtggccg cgttcgccag gctcgccggc
     3301 gcgcgcgagg ttgtggtgag cgagatgaac gcgacaaggc gcgagaagtc gatggcaatg
     3361 ggcgcgaccg cggttatcga tccggcatcg gcggacaccg ccgaagcctt cgccaggatc
     3421 gcgggctgtc cgcccgacgc gatctttgaa tgtgtcggcg tgcgcggcct catccagcaa
     3481 tgcatcgacc tgtcgcgtcc gcgcggccag atcgtggttg tcggcgtctg catgatggag
     3541 gacacggtcg tgccgatctc ggcgatcctc aaggaactgg atatccagtt tgtgctcggc
     3601 tatctcagtg aggatttcga tatcgtgctc gatgcaatcg ccaagaagac catcaacgcc
     3661 atgccgctgg tgtccgacat cgtcggtttc gaggaactgc cggccgcgtt cgaggccttg
     3721 cgcaggccga aaggccagat caaggttctg atccggcccg gcgtctaatc ggatctattg
     3781 ctgctttgtg aatcgagtgc agcgtatttt cgcgagactg cacgagttcg ttgctgggga
     3841 gggcgcaaca agattccggt tcgaaaacca gcgggcggag cggcctagga ctggaccggt
     3901 gcaaaggctt cttgcgaaag gcggtcaagc gccagacggg tgcgcctgat gtgaagatgc
     3961 atcacccgtt ccgacaggcg aggattgccg ctcagaacgg cgcgcgaaat cgcctcatat
     4021 tcctggaact gcttttcgcg ctgacgcgcc gtcacataag gctggaactg caagcgcagc
     4081 aggtggatct gcatcagcgg catcaaacgt ttcagttcac cattgccgcc gatgcgaagg
     4141 agtgtgtcat agtaggagcg gcgctggtca aggaatgcga tgctgtcacc ctgatcccgg
     4201 aacgtcatga gcttctcgtg ctcggcggtg aaggtctcgc ggttcccttt caggtcgata
     4261 tgctgcgctg caagccgtgc gaccaagccg gttagaactt ccagtatcat cagcgtatcg
     4321 cgcacctcgt cgcgcgtcat ggcgcggacg aaggctccgc gatggcggtt cagcgtcacg
     4381 acgccttccg cggcaagccg tttcagtgcc tcgcggaccg gcccgcggct cacgccaaga
     4441 ttgcgcgtca aatccgcctc gacgagacgc tggccgggca ccaggcggcc ggcgtggacg
     4501 ccgcggatga tggcattgac gacgatgtca ggaaaactcg cgcgttcgcc ttccgatcgc
     4561 gtatcgacag catcagtcac gccgcgagtg ggtacgatca gtccaagact ttctgccatc
     4621 ctgctaatgc ccgcttctca cgttcattct cactctatag gcgggaaatg attctgtggc
     4681 aaacgcgata tatgcattat tgtcgtacaa aattgtcggt cagcaatgtg cgaattggcc
     4741 ttgcaatcct gttctagaca gtaattggag aaaacgggga ccgtcgtccg tcccgaggac
     4801 ggacgccaag agggaggggg acccttccgg gtcgagggga attctcaggt cgaggcagcc
     4861 cgagtgcagc aagcggtggc gccggaacgc cgatgtgcta tcatattgac ctacgataat
     4921 gtcatacaat aggaggagac aatgcgtgga cgatggttgc ttgctttgtt cgcgttggcc
     4981 ctgggtacac tttcggcccg ggctggggat gtttatgtca tcgacaatgc ggcgggaaaa
     5041 accccggtgg cgatcaaaaa ggacgccttc gcgctgaccg cagtgccggc catgggggag
     5101 ggcaagccga atgagggctt cgcttacacg acggcccttg agctcgccgg aaccggcgtt
     5161 cagatcgcaa ggggccgcgt cgaaccgggt ggaaccgtgg caacccacga cggacgtcag
     5221 caatacattc tctatgtcat aggcggcact gggacgcttg gtcttgttga ccagaccggc
     5281 gcgacaatcg gcgagatcaa gtacaagccc gacgacgtaa tcgtcttcca gcccaacacg
     5341 cttcacaact ggaaaaatgc cagtgccgcc ccgttcgagt ttctcggagt ggatctcgct
     5401 ccaccccgga aatagcgctc gcctggcgcg cggcctcggt cggcattggt ggcagagtcg
     5461 ttctgctgcc aatgcttcct tgaggttatt acgctggtcc gcccacgatg cggatcagaa
     5521 acccacgatc gccggcccag gattggaaat cctcatgctc tattccgagt ccaacaaagt
     5581 catcgagctg aaacgtcgtc tccagtcgtt tatggaccgc catgtctatc cgaacgaaga
     5641 gcggttctat cagcaggctg aggagttggg accctggaaa gtttatccag ttgtcgagga
     5701 attgaagccg aaggccaggg cggaaggcct ttggaatctc ttcctgccgg aaagcgaaca
     5761 cggcgccggc ttgacgaatc tcgaatatgc gccgctatgc gagatgatgg gtcgctcgca
     5821 tctcgcgcca gaggtcttca actgttcggc acctgacacc ggaaacatgg aagtgctggc
     5881 gcgctacggt acgaaggagc accagcagcg ctggctcaag ctgctgctgg ccggcgaaat
     5941 ccgctcctgc tttgccatga ctgaaccggc cgtcgcctcc agcgacgcca ctaacattga
     6001 aagctcgatc gttcgtgagg gcgaccacta cgtcgtcaac ggccgcaagt ggtacaccac
     6061 caatgccacc gatccgcgct gcaagatctg tatcttcatg ggcaagactg atcctgacaa
     6121 tccggaccgc catcgccagc aatcgatgat tctcgtgccg atggatacgc ccggcgtcga
     6181 agtgttacga ccgataccgg tattcggatt ctacggtgtc cctgatcgcg cgtcggaagt
     6241 cgtcttcaac aatgtgcggg ttcccgcgac gaacattctg cttggcgagg ggcgcgggtt
     6301 cgagatcgca caaggccggc tcggtcctgg aaggattcat cactgcatgc ggctgatcgg
     6361 acttgccgag cggacgcttg agaaaatgtg cgttcgaacc agaaaccgcg ttgctttcgg
     6421 taagccagtt tccgaacaga ccgtgacgca ggaacgtatc gcggaatcac gcatcatgat
     6481 cgagcaggcg cgcttgctga ccctcaacgc ggctcatatg atggatacgg tgggaaacag
     6541 aacggcgaaa gccgagatag caatgattaa ggtcgcggta ccgaacatgg cgtgccaggt
     6601 catcgactgg gcaatccagg cgcatggtgg cggcggcacc agcaacgatt tcggtcttgc
     6661 cgcggcctac gccacggccc gccttcttcg cctggccgat ggtcccgatg aagtccatcg
     6721 gaaccaaatt gctcgtctgg aactgcgcaa acacaatatt actcaaggcg ggagcagcga
     6781 ttgaattacc gggctttcga agtggagatg agttcggcct gcagttcgcg cttaagcacc
     6841 ttgccgtatt tgctcttcgg caaagagctg gggaaacggt attccctcgg tcgcttgaag
     6901 cgtgcgatct ccgaaaggca gtgcgtgtcc agctcggaag cggtcacctg tgatccggtt
     6961 tgcgctacga cgaaagccac gatcttttcg ccccagaccg gctccggtgt gccgactacg
     7021 gcaacctccg cgaccgccgg gtgcctcagc aacacctcct cgacctcccg cggataaatg
     7081 ttcgagccgc cgctgatgat caactccttc gcccggcctt tgagcgtcaa cagaccgtct
     7141 tcatcgatcg cgccgaggtc gcctgtcatc aaccatccgc caacaagcgc tgctcgcgtg
     7201 ctctgctcat cgccccaata gccgctcatc acctgtgtgt accgaaaacc ttatccggat
     7261 ttttcgcgta acagaatcgg ctaaactggc gcgatgatcg cgctgatttg gtttgttctg
     7321 gccgtgctgg cctctccgtt caagtcgaag agcaggcttg aggcggagaa tgcggtgctc
     7381 cgacatcagc tgatcgtttt gcggcgcaag gtgagggggc gagcccagct taccaacaac
     7441 caccgctggt tcctcgtcca gatgtatcga tggtttccat cgatcctgac ggtcgttacg
     7501 attgtccaac ctgaaacgct ggtgcggtgg catcgggccg gctttcgtcg ctattggcgt
     7561 tggaaatcca acccgcgggg ag
//
LOCUS       DASWGM010000598         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1160214,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000598 DASWGM010000000
VERSION     DASWGM010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160214"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VGA15_17000"
     CDS             complement(<1..600)
                     /locus_tag="VGA15_17000"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX9589431.1"
                     /translation="MLGEGGTPISEFEAAVRKFLAREERGGIELKRYRSIIDALDGDF
                     AAEAREAQQSGDHLVDGNITAASWISRNCGMSVTSAADRLCVGEQLRSLPKVAAALSS
                     GEIGYQSASVLCHLRDQLGEKRYLFDEEEMLDYARRFSVFHLRMLCRVARHAADPDGF
                     FTEAEQNYLHRRFHISLMSDGMFAVDGILDPECGAALKTA"
     gene            complement(787..1593)
                     /locus_tag="VGA15_17005"
     CDS             complement(787..1593)
                     /locus_tag="VGA15_17005"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589432.1"
                     /translation="MEGFWTKVALRIWVALILLFLFIPILLIFFYAFNTSNIESWPIP
                     GLTLKWFTASWDDAEIRTALGLSVQAGLAATALSLVLGSCAAFAIHRMTFFGRDAISL
                     LFVLPLALPGIITGIALNAFFSFGGFPLSFWTIVVGHTTFCIVVVYNNVLARLRRSQG
                     SLIEAAMDLGANGLQTFRDITLPLISTSVVAGAMLAFALSFDEVIVTTFTAGAQNTLP
                     IWIFGAIRLGQRLPEVNVVVSFVILVTIVPVVIAARLTGGGGTARIPAAR"
     gene            complement(1596..>2177)
                     /locus_tag="VGA15_17010"
     CDS             complement(1596..>2177)
                     /locus_tag="VGA15_17010"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589433.1"
                     /translation="AYFMARVATRRTQTILFAAVLLPLWASYLARVYAWILILNHSGI
                     LNWGLQSIGLPPANIGYTIPAMWIVFSYIWLPFMIIPTYAALERVPESLLEAAADLGA
                     PRWRAVLDVVLPLAIPGVVAGSIFTFSLTLGDYITPILVGGAGSTFIGNVVYSSVGIA
                     NNVPFAAALAMVPVAIMAVYLLIARRLGAFEAL"
BASE COUNT          472 a          696 c          672 g          337 t
ORIGIN      
        1 cgccgtcttc agcgccgctc cgcattcggg gtcgagaatg ccgtcgacgg caaacatgcc
       61 gtctgacatc aagctgatgt ggaagcggcg gtgcaagtag ttctgctccg cttcagtgaa
      121 gaagccgtcc gggtcggcgg cgtgcctggc gacgcggcaa agcatgcgta gatgaaagac
      181 cgagaaccgg cgcgcatagt cgagcatctc ctcttcgtcg aagagatacc gcttctcgcc
      241 gagctggtcg cgcaggtggc agaggacaga ggccgattga tagccgatct cccccgagct
      301 caaagcggcg gcgaccttag gcagcgaccg caactgctcg ccgacgcaga gccggtcggc
      361 ggcggaggtc accgacatgc cgcagttccg gctgatccat gaggcggcgg tgatgttgcc
      421 atcgacgagg tgatcgcctg actgctgggc ctctcgtgct tccgccgcaa agtcgccgtc
      481 gagggcgtcg atgatcgatc ggtagcgctt cagttcgatg ccgcctcgct cctcgcgagc
      541 gaggaatttg cgaaccgcag cctcgaactc agagatcggt gtacccccct cccccagcat
      601 ttgagcagca taccaaacat tcgttcgggc gtcaatgcgt taacaccaaa aacgtgcttg
      661 aaaaccaacc aatccgcacc caactccctc ttcttagttc ttacggaacg cccccacccg
      721 gcgaggccgc gcttgacaac cacccaggcc ccaaccccca ctcccttttc ccagtctttc
      781 tgtcgttcag cgagcagcag gtatcctcgc cgtcccgccg ccgccggtga ggcgggcggc
      841 gatcaccacc ggcacgatcg tgaccaggat cacgaacgac accaccacgt tcacctctgg
      901 cagccgctgc cccagccgga tggcgccgaa gatccagatc gggagcgtgt tctgcgcgcc
      961 cgcggtgaac gtcgtcacga tcacctcgtc gaacgaaagt gcgaaggcaa gcatcgcgcc
     1021 ggcaaccact gacgtcgaga tgagcggaag cgtgatatcc cggaacgtct gcaagccgtt
     1081 cgcgcccagg tccatagccg cctcgatcag tgagccctgc gagcgacgca aacgcgccaa
     1141 cacgttgttg tacacgacca cgatgcagaa ggtcgtgtgg ccaacgacga tggtccagaa
     1201 cgaaagcgga aagccgccga agctgaagaa ggcgttgagc gcgatgcccg tgatgattcc
     1261 cggcaacgcc aatggcagca cgaaaagcaa gctgatggcg tcccggccaa agaatgtcat
     1321 gcgatggatg gcgaacgcgg cacacgagcc aagaaccaaa gacagcgcag tggccgccag
     1381 ccccgcttgc accgaaaggc ccaacgccgt gcggatctcg gcgtcatccc atgacgccgt
     1441 gaaccacttg agcgtgagac ctgggatggg ccagctctcg atgttggacg tgttgaatgc
     1501 atagaaaaag atgagcagga tcgggatgaa gaggaaaagc aggatgaggg cgacccatat
     1561 cctcagcgcg accttcgtcc agaagccttc cacgactaca aggcctcgaa cgcgcccagg
     1621 cgccgcgcta tcagcaggta gacggccatg atcgccaccg gcaccatcgc cagggccgcc
     1681 gcaaacggca cgttgttggc gatgcccacg ctgctgtaga cgacgttgcc gatgaacgtc
     1741 gatcccgcgc ctccaaccag gatcggcgtg atgtagtcgc cgagcgtcag tgagaacgtg
     1801 aatatcgagc ctgccaccac gccgggaatc gcaagcggaa ggaccacgtc gaggaccgcg
     1861 cgccatcgcg gcgcgccgag gtccgcggcc gcctcgagca gcgactctgg cacgcgttcg
     1921 agggcggcgt aggtcgggat gatcatgaac ggcagccaga tatacgagaa gacgatccac
     1981 atcgcgggga tcgtgtagcc gatgttggcc ggcggtaggc cgatcgactg caggccccag
     2041 ttgaggatcc cgctgtggtt gaggatgagg atccacgcgt agacgcgagc gaggtacgac
     2101 gcccacagcg gcagcaacac cgccgcgaac aggatcgttt gagttcgccg cgttgcgact
     2161 cgcgccatga aataggc
//
LOCUS       DASWGM010000599         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_74893,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000599 DASWGM010000000
VERSION     DASWGM010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74893"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..824
                     /locus_tag="VGA15_17015"
     CDS             144..824
                     /locus_tag="VGA15_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9589434.1"
                     /translation="MTNDMPERPTPHPAATRRIPFAIGAVLIGAVIGFAAVYGIGGLK
                     RNAAGDSACRGAVDLAGRLAPLAHGEVAALTMATTPLLLPDLAFEDADGKPKKLSDWR
                     GRTVLVNLWATWCVPCRREMPALDSLQTRLGGPDFEVVAVNIDTRDPEKPKNFLKDAN
                     LTRLSYFSDQKAKVFQDLKAIGKALGMPTSVLVDGKGCEIATIAGPAEWASDDAVKLI
                     TAAMMPPG"
     gene            complement(942..1145)
                     /locus_tag="VGA15_17020"
     CDS             complement(942..1145)
                     /locus_tag="VGA15_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589435.1"
                     /translation="MDMMSMVSSLLAMKAGGTQSQIAATIMKSNADAEKSTVLTLLGA
                     GGQGVSSLANVGAGVGGNLDISA"
     gene            complement(1337..2215)
                     /locus_tag="VGA15_17025"
     CDS             complement(1337..2215)
                     /locus_tag="VGA15_17025"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX9589436.1"
                     /translation="MAMIKKVGVIGSGQMGNGIAHVAALAGFDVVLNDVSLDRLKSAM
                     ATINGNLSRQVTKKIITEDARKQALDRIVSAETMEGLADCDIVIETAVEKEEVKRKIF
                     HEVCAVLKPEAIVASNTSSISITRLAASTDRPERFIGIHFMNPVPLMELVELIRGIAT
                     DDQTFEASKEFVTRLGKHIAVSEDFPAFIVNRILLPMINEAIYTLYEGVGNVEAIDAA
                     MKLGAHHPMGPLELADFIGLDTCLSIMQVLHEGLADSKYRPCPLLVKYVEAGWLGRKT
                     QRGFYDYRGSKPVPTR"
     gene            complement(2308..3258)
                     /locus_tag="VGA15_17030"
     CDS             complement(2308..3258)
                     /locus_tag="VGA15_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9589437.1"
                     /translation="MTTLLIAEHEHSTLKDSTNKALTAATQLGAEVHVLVAGGGEGTK
                     AAAEAAAKLAGVTKVLLAEGDAYAHDLAEPLAALIVALAPSYDAFVAPATSRFKNVMP
                     RVAALLDVMQVSEIIKVVSPDTFERPIYAGNAIQTVKSKDAKKVITVRTSTFAAAGDG
                     GSATVENAASAADPGLSSFVGEEVAKSDRPELTSAKIIVSGGRAMQSRENFAKYIEPL
                     ADKLGAGVGASRAAVDAGYAPNDWQVGQTGKVVAPELYIAIGISGAIQHLAGMKDSKV
                     IVAINKDEDAPIFQVADYGLVADLYQAVPELTEALGKLGK"
     gene            complement(3259..>3729)
                     /locus_tag="VGA15_17035"
     CDS             complement(3259..>3729)
                     /locus_tag="VGA15_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX9589438.1"
                     /translation="PLAVAKILKAIADEEKPGLIILGKQAIDDDSNQTGQMLAALLGW
                     SQATFASKLEVDGTDFKVTREVDGGLQTLKLKGPAIVTTDLRLNEPRYASLPNIMKAK
                     KKPIADKSASDYGVDLTAHLEILKTSEPPGRKGGVKVKDVTELVSKLKNEAGVL"
BASE COUNT          689 a         1190 c         1129 g          721 t
ORIGIN      
        1 gcgctcggtg aaccggccgc cccacatctt gttgctcatg acttgttgct cacgacctgc
       61 cgctcatggt cacgcccgcg aagcgaacgc gctgtgccgt taccggcgcc gccaaaactc
      121 tgcatagccg tatctgatac aggatgacaa acgatatgcc tgaacgaccc acacctcatc
      181 cggctgccac gcgccggata cccttcgcga tcggggcggt gctgatcggg gcggtgatcg
      241 gatttgccgc cgtatacggg attggcgggc tgaagcgcaa tgcggcgggc gattcggcct
      301 gccgcggggc ggtcgacctt gccggcaggc tcgcgccgct ggcgcatggc gaggtcgccg
      361 cgctgacgat ggcgacaacc cccttgctgc tgccggacct cgctttcgag gacgccgacg
      421 gcaagcccaa aaagctctcc gactggcgtg gacgaaccgt gctggtgaac ctgtgggcca
      481 cctggtgcgt gccctgccgc agggagatgc cggcgctgga tagcctgcaa accaggctcg
      541 gcggacccga cttcgaggtg gtcgccgtca acatcgatac ccgcgatccc gaaaaaccga
      601 agaatttcct caaagacgcc aatctgaccc ggctcagcta ttttagcgac cagaaagcca
      661 aggtatttca ggatcttaag gcgatcggca aggcattggg aatgccgacc tcggtgctgg
      721 tagatggcaa gggctgcgag atcgccacca tcgccggacc tgcggaatgg gccagcgacg
      781 acgccgtcaa gctgatcacg gcggcgatga tgcctcccgg ctagccttgt cattccgggg
      841 cgggagtgaa acgagcgagc ccggaatcca taacccctgc ggggagtatg gattccgggc
      901 ctgcgccaag aggcgcatcc cggaatgacg aaagaagaga ttcaggccga gatatcgaga
      961 ttgccgccga caccggcgcc gacattggcc agcgacgaca cgccctgtcc gcccgcgccc
     1021 agcagggtaa gaaccgtgga tttctccgca tccgcattcg atttcatgat cgtggctgcg
     1081 atctggctct gcgtgccacc cgccttcatg gcgagaaggc tactgaccat actcatcata
     1141 tccatgcgca acactcccga cgccggcaca ctaccccggc gctagttaat gttacgtgtc
     1201 ccggacgcgg tgcagcgctt cttcagcggt gcaccgcaga gccggaaccc acgaacagcg
     1261 gagtgcaatt tacaatggcg cccggatcag cagcggaccg ctgacgcctg cgcggcatcc
     1321 ggggaacgcc gcctaatcac cgcgtcggga ccggcttgct gccgcgatag tcgtagaagc
     1381 cgcgctgggt cttgcggccg agccagccgg cctcgacgta cttcaccagc agcgggcacg
     1441 gccgatactt cgagtcagcc agaccctcat gcagaacctg catgatcgac agacacgtat
     1501 cgagaccgat gaaatcggcc agttccagcg gccccatcgg atggtgcgcg ccgagcttca
     1561 tcgcggcatc gatcgcctcg acgtttccga ccccttcata gagcgtataa atcgcctcgt
     1621 tgatcatcgg cagcaggatg cggttgacga tgaaggccgg aaaatcttcc gagaccgcga
     1681 tgtgcttgcc gagcctcgtc acgaattcct tcgacgcctc gaaggtctgg tcatcggtgg
     1741 cgatgccgcg gatcagttcg accagttcca tcagcggcac cggattcatg aagtgaatgc
     1801 cgatgaaacg ctcgggccgg tcggtggatg cggccagccg cgtgatcgag atcgacgagg
     1861 tgttggaggc gacgatggcc tccggcttca gcaccgcgca gacctcgtgg aaaatcttgc
     1921 gcttgacctc ttccttctcg accgcggtct cgatcacaat atcgcaatcg gcgaggccct
     1981 ccatcgtctc ggcggaaacg atccgatcca gcgcctgctt gcgggcgtcc tcggtgatga
     2041 ttttcttggt gacctggcgt gacagattgc cgttgatggt cgccatcgcc gacttcaggc
     2101 gatcgagcga cacatcattg agcacgacgt cgaaaccggc cagcgccgcc acatgcgcga
     2161 tgccgttgcc catctggccc gagccgatca cgccgacctt cttgatcata gccatcttgt
     2221 catctcaccg gagcgacgcg catcgcgcgc ccgcccatcc cttatagcac cggccgaagt
     2281 cccctcaccc ggttgtttaa attatcgtta ctttccgagt ttgccgagcg cttcggttaa
     2341 ttcgggaacc gcctggtaga ggtccgcgac cagaccgtaa tcggcgacct ggaaaatcgg
     2401 cgcatcctcg tccttgttga tcgcgacgat caccttggaa tccttcatgc cggcaagatg
     2461 ctgaatggcg cctgaaattc cgatggcgat ataaagttcc ggcgccacga ccttgccggt
     2521 ctggccgacc tgccagtcgt tcggcgcata tccggcatcg accgcggcac gcgaggcgcc
     2581 gacgccggcg ccgagcttgt cggcgagcgg ctcgatatat ttggcaaaat tctcgcggct
     2641 ctgcatggcg cggccgccgg agacgatgat cttggccgat gtcagttccg gacgatcgct
     2701 cttggcaact tcctcgccga caaaactgga gagaccgggg tcggccgccg acgcggcgtt
     2761 ttccaccgtt gcgctgccgc cgtctccggc agcggcgaag gtcgaggtgc ggacggtgat
     2821 caccttcttg gcgtccttcg atttcaccgt ctggatggcg ttgccggcat agatcggccg
     2881 ctcgaaggta tcaggcgaaa ccaccttgat gatttccgat acctgcatca catcgagtag
     2941 tgctgcgacc cgcggcatga cattcttgaa gcgcgacgtc gcgggcgcga cgaaagcgtc
     3001 ataggaaggg gccagcgcca cgatcagcgc ggccagcggc tcggcgaggt cgtgcgcata
     3061 ggcatcgcct tcggcaagca aaactttcgt gacgccagca agcttggccg cggcctcggc
     3121 ggccgccttg gtgccctcgc cgccgccggc gaccaacacg tggacctcgg cgcccaattg
     3181 ggtggccgcc gtcagcgcct tgttggtgga gtccttcaag gttgagtgtt cgtgttcggc
     3241 aatcaacagc gtggtcatct agagaacccc ggcttcgttc ttgagcttcg acaccaactc
     3301 tgtgacgtcc ttgaccttga cgcctccctt gcggcccggc ggttcggatg tcttgaggat
     3361 ctccagatgg gcagtgagat cgacaccgta atcggatgca ctcttgtcgg cgatcggctt
     3421 cttctttgcc ttcatgatgt tgggcagact ggcgtagcgc ggctcgttca gccgcagatc
     3481 ggtagttacg atcgccgggc ccttgagttt caacgtctgc aagccgccgt cgacctcgcg
     3541 cgtcaccttg aaatcggttc catcgacttc gagcttggag gcaaaggtcg cctgcgacca
     3601 cccgagcagg gcggccagca tctggccggt ctggttactg tcatcgtcga tcgcctgttt
     3661 gccgagaatg atcaggcccg gcttctcttc gtcggcgatg gctttcagaa ttttcgccac
     3721 cgccaaagg
//
LOCUS       DASWGM010000600         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1737402,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000600 DASWGM010000000
VERSION     DASWGM010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737402"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VGA15_17040"
     CDS             complement(<1..547)
                     /locus_tag="VGA15_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589439.1"
                     /translation="MARDSQAALVALNRFGFGARGGASGDFVNAASDPRGFVKAELNR
                     PNGVLLEVPGLLSTPAYGQAVFAYQAEVKQAREAAAKSGAPVSEAAPAQPAADPKAPR
                     RNLSLNSIAMDIAVKDPAAKDLAAKDLATKDLATKDPAMKAPENGNTNAAMAPTDAMQ
                     PNAAKQPTQPLNIIQKTYRAEA"
     gene            650..868
                     /locus_tag="VGA15_17045"
     CDS             650..868
                     /locus_tag="VGA15_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589440.1"
                     /translation="MTNRLETPMSRRLACSRCGTEFACRPEGPCWCTEEIARLPMPVE
                     GEDCLCRDCLRSAASQATNSTSNAASSS"
     gene            complement(1054..1701)
                     /locus_tag="VGA15_17050"
     CDS             complement(1054..1701)
                     /locus_tag="VGA15_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9589441.1"
                     /translation="MPNLENLRKQAKLILRWHRDRYYPVAAQIRSGLPRYGQMSDPEI
                     LAQSFKLSDAQELVARQHGFESWQALKTGLATMSDHADTTITKAAIAGAEPQLFVADI
                     KSSCDFFTSKLGFVIVFTYGEPPFYAQVKRDAACVNLRCVEQPVIDAELRDREQLLAA
                     SLTVATSEEIKTLFLEFQAAGVTFFQTLKREPWGARDFIVKDPDGNLLLFAGPAE"
     gene            complement(1875..2570)
                     /locus_tag="VGA15_17055"
     CDS             complement(1875..2570)
                     /locus_tag="VGA15_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9589442.1"
                     /translation="MPLKLFELVGTDEARPFSPFCWRTRMALAHKGLSAETIPWCFTE
                     KDAIAPHNSEKVPVLIDGESSVADSWAIANYLEDRYPDRPSLFGGAGGRAMGRMINWW
                     GDVTVIGGMFPLIVADIPGHLKPVDAVYFRQSREARFGKPLEEVMAGREKTVEGFRRS
                     LDPMRLTLKTQAYLGGDAPNYADYIVFGGFQWARVVSPFRLLAENDPVHAWREKLLDA
                     FDGMARKSPGYPV"
     gene            complement(2705..>2987)
                     /locus_tag="VGA15_17060"
     CDS             complement(2705..>2987)
                     /locus_tag="VGA15_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX9589443.1"
                     /translation="VLNKRLSNREFIAGDYSIADMASYPWVVPYKNQSQNIDDFPHLK
                     RWLETIRERPATVRAYALAKEVNPNFGQPAIRTDEERKLLFGQTAAVVK"
BASE COUNT          526 a          926 c          925 g          610 t
ORIGIN      
        1 gcgcctcggc gcggtaggtt ttttgaatga tgttgagcgg ctgggttggc tgcttcgctg
       61 cgttcggctg catcgcgtca gtcggtgcca tcgctgcgtt ggtgttacca ttctccggcg
      121 ccttcatcgc cgggtccttt gtcgccaagt cctttgtcgc caagtccttt gccgccaagt
      181 cctttgccgc cggatccttt accgcgatgt ccatggcgat gctgttgagc gaaaggtttc
      241 gccgtggcgc cttgggatcc gctgccggtt gcgccggcgc ggcctcggac accggcgcgc
      301 ccgatttcgc ggccgcctcc cgcgcctgct tgacctctgc ctgatacgcg aataccgcct
      361 gtccatatgc cggagtcgac aacagtccgg gtacctcgag cagcacgcca ttggggcggt
      421 tcaattcggc tttgacaaag ccgcgcggat cggaggcggc gttgacaaaa tcacccgacg
      481 cgccgccgcg ggcgccaaat ccgaagcgat tgagcgcgac cagagcagct tgcgaatcgc
      541 gggccatcgg atttcccttg cacggtcgcg ggcgtatatt gatggcggat gataccgccg
      601 cccggcgtga accggacatg aaaaacacgg ccaaggtttg gttcgattca tgacaaatcg
      661 gttagaaact ccgatgtccc gccgcctcgc ttgttcccga tgcggaacgg aattcgcctg
      721 caggccggaa ggcccgtgct ggtgcaccga ggaaatcgcc cgattgccga tgccggtcga
      781 gggcgaggac tgcctgtgcc gggattgttt gcggagcgcg gcgtcgcagg caacgaattc
      841 gacaagtaat gccgcctcgt cctcctaatc gctggctccg cgttccccgg atgctgcgca
      901 gcgccatcag cgcgtttacg cgcgtctttg acgcgctatg tcttgcggcg tggtgcgctg
      961 ctgatccggg gtccatggat gggtgggtcc cggctctgcg gagcagcgtg aaggacgctg
     1021 caccgcgtcc gggacacgtg catcacgcga agcttactcc gccggtcctg cgaacagcag
     1081 cagattaccg tccgggtcct tcacaatgaa atctcgcgcg ccccagggct cgcgcttgag
     1141 cgtttggaaa aacgtcacgc cggcggcctg aaactcgagg aacagcgttt tgatttcctc
     1201 cgaggtcgcg acggtgagcg aggccgccag caattgctca cggtctctca attcggcatc
     1261 gatcaccggc tgctcgacgc atcgcaggtt gacgcacgcc gcatcgcgtt tgacctgcgc
     1321 atagaacggc ggctcgccat aggtgaacac aatgacaaag ccgagtttgc tggtgaagaa
     1381 atcgcaggat gacttgatat cggcgacgaa caattgcggc tcggcaccgg cgatggcggc
     1441 tttggtgatc gtcgtatctg catgatctga catggtggcg agtcctgtct tgagcgcctg
     1501 ccagctttca aaaccatgtt gccttgcgac cagttcctgc gcatcgctga gcttgaagct
     1561 ttgcgcgaga atttcggggt cgctcatctg gccgtaacga ggcaagcccg acctgatctg
     1621 cgccgcgacc ggataatacc tgtcacgatg ccaacgcaag atcagcttgg cctgcttcct
     1681 gaggttttcg aggttcggca tgggcgcgct acggttgaca ttgctttaag cggtcaatgc
     1741 gtaggtgggc attgcccctt cggcttaaag ccgatgcgcc ctacatgcag cgccggcagc
     1801 atggccgagg tgccggacat ttgtcaatat ccgtggggac atcacatccg aaacaccacg
     1861 aagggcgcgg caccctacac cggatagccc ggcgacttgc gcgccatgcc gtcgaacgcg
     1921 tccagcagtt tttctcgcca cgcatggacc ggatcgttct cggcgagcaa tctgaacgga
     1981 ctgaccacgc gcgcccactg gaatccgccg aacacgatat agtcggcata gttcggcgca
     2041 tcgccgccga gataggcttg cgtcttcaac gtcagccgca tcgggtcgag actcctgcga
     2101 aagccctcga cggttttttc acgcccggcc atgacttctt ccagcggctt gccgaagcgg
     2161 gcttcgcggc tctgccggaa atagaccgcg tccaccggct tcagatggcc cgggatatcg
     2221 gcgacgatca gcgggaacat gccgccgatc acggtgacgt cgccccacca gttgatcatc
     2281 cgccccatgg cgcggccgcc ggcgccgccg aacagcgagg gacggtccgg atagcggtct
     2341 tccagataat ttgcgatcgc ccaggaatcg gcgaccgagc tttcgccgtc gatcagcacc
     2401 ggtaccttct cggaattgtg cggggcgatc gcgtctttct cggtaaagca ccaggggatg
     2461 gtttccgccg acaagccctt gtgcgccagc gccatccgcg tccgccagca gaacgggctg
     2521 aacggacgcg cctcgtcggt gccgaccagt tcgaagagtt ttagtggcat tagagcggtc
     2581 cccgcgaatg cacgcgtgcg ccaatttagc gtcgtcattg ccggacttga tccggcaatt
     2641 catcttctta gaagatggat acgcgggtca agcccgcgta tgacaagttg attcgccgcg
     2701 ctgtctactt caccaccgcc gcggtctggc cgaacaacag cttgcgctct tcgtcggtgc
     2761 ggatcgcggg ctggccgaaa ttcggattga cctccttggc cagcgcatag gcgcgcaccg
     2821 tggccggccg ctcgcggatg gtctcgagcc agcgcttgag atgcgggaag tcgtcgatgt
     2881 tctggctctg gttcttgtac ggcacgaccc agggatagct cgccatgtcg gcgatcgaat
     2941 agtcgccggc gatgaactca cggttcgaga gccgcttgtt gagtacg
//
LOCUS       DASWGM010000601         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_903609,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000601 DASWGM010000000
VERSION     DASWGM010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903609"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VGA15_17065"
     CDS             <1..1527
                     /locus_tag="VGA15_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9589444.1"
                     /translation="TESHNTNGLNALKNFVMDVDGYIGLYSIQNGNEQLIECLQSEVD
                     ADIQLNHRVLKVGKTTAGRYQLNMMNGKGPETRDFDLVVMCLPHSWLATMRWDGEALR
                     KSMVKHVAYFDRPAHYLRVSILFDTPFWGEKIPGAWFMSEAFGGCCVYNEGARHDVGK
                     HGVLNWLIAGSDALAFANLSDQELIDAALKSLPASLGNAREHFMEGKTHRWLSSVNAL
                     PGAMPARDVMTNHRPDPKQHPGLVVVGDYLFDSTLNGLLDSSDAATDIILTEMMRLRR
                     ARGEGTRPASDKIDRQYFENYRGLGPYQEVWSRFTDPAYLTDLIKIVWNRARGYKLLV
                     AGSASGELVGALRDRGIDAWGIENNRAIHAHTPKALKKFNKLGSIVEMPFNDGEFDFV
                     FETSLCHVAEKQVPRAVRELNRVVKTGLVFGSVTSDMAPALIDQYDLLRGVKKLGTWW
                     EWSELFFSNGFDLSMHRRDCTDAIWAATLAANKGPGQWYADSDSLRYSFFDKVKADDD
                     "
     gene            1693..>2206
                     /locus_tag="VGA15_17070"
     CDS             1693..>2206
                     /locus_tag="VGA15_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589445.1"
                     /translation="MAPKPPSSDDQIPDSAEKPVAPELDQKLAAGADRVIGDKTAGSP
                     PPDDEAEDDDELDIDDEDDDEDLVVFTAKEAAGALATVYAFVKPYLKNYKKILAFVGL
                     GVLVETLFNVIMPLSLKFLIDDALGEEDFEALVKILSVLAAAGIVTSVVAVWYERWDA
                     ELAATLISDVR"
BASE COUNT          447 a          652 c          684 g          423 t
ORIGIN      
        1 accgagagcc acaacaccaa tgggctcaat gcgctgaaga acttcgtcat ggatgtcgac
       61 ggctatatcg gcctgtattc gatccagaac ggcaacgagc aattgatcga atgcctgcag
      121 tcggaggtcg acgcggacat ccagctcaat caccgggttc tcaaggttgg caagaccacg
      181 gccggccgct atcagctcaa tatgatgaac ggcaaaggac cggaaaccag agatttcgat
      241 ctcgtggtga tgtgcctgcc gcactcctgg ctcgcgacca tgcggtggga cggcgaagcg
      301 ctgcgcaagt cgatggtcaa gcatgtggct tatttcgatc ggcctgcgca ttatctgcgg
      361 gtctcgatcc tgttcgatac cccgttctgg ggagagaaaa tcccgggcgc atggttcatg
      421 tcggaagcgt tcggcggctg ctgcgtctac aacgagggcg cccgtcatga cgtcggcaag
      481 cacggcgtcc tgaactggct gatcgccggt tccgacgcgc tggcctttgc caacctcagc
      541 gatcaggaac tgatcgacgc cgcactgaag tcgctgccgg cctcgctcgg taacgcccgc
      601 gaacatttca tggagggcaa gacccatcgc tggctctcgt cggtgaatgc gctgccgggc
      661 gccatgccgg cgcgcgacgt catgaccaat caccggcccg atccaaaaca gcatcccggc
      721 ctggtcgtgg tcggcgacta tctgttcgac tcgacgctga acggactgct cgactcctcc
      781 gatgccgcca ccgacatcat tctcaccgag atgatgcggc tgcgccgcgc gcgcggcgag
      841 ggtaccaggc ccgcatccga caagatcgac cggcagtatt tcgagaacta tcgcgggctt
      901 ggtccctatc aggaggtctg gagccggttt accgatccgg catatctcac cgacctcatc
      961 aagatcgtgt ggaacagggc gaggggttac aagctcctgg tggccggatc cgccagcgga
     1021 gaactggtgg gtgcgctgcg cgatcgtggc atagatgcat ggggcatcga gaacaaccgc
     1081 gcgatccatg ctcacacgcc gaaagcgctg aagaagttca acaagctcgg atcgatcgtc
     1141 gaaatgccgt tcaacgacgg cgaattcgat ttcgtgttcg agaccagtct gtgtcatgtc
     1201 gcggaaaagc aggtgccgcg ggcggtgcgc gagttgaacc gcgtcgtcaa gaccgggctg
     1261 gtgttcggat cggtgacatc ggacatggcg ccggcattga tcgaccagta cgacctgctg
     1321 cgcggggtca agaagctcgg cacctggtgg gaatggtcgg agcttttttt cagcaacggg
     1381 ttcgacctgt cgatgcatcg ccgcgactgc accgatgcga tctgggccgc aacgctggcc
     1441 gccaacaagg ggccaggtca gtggtatgcg gattccgaca gcctgcgcta ctcgttcttc
     1501 gacaaggtga aagccgacga cgattgagcc agtaaacgcg tcgtccccgc ggttcgccgg
     1561 gacgacgcgt ggaaagttct tcgctgttgc tttgccgaat gcgtgccaat cgcataggat
     1621 cgacgcggtg gcactctcgg ccatctccct cgattttgcg gtcatgctgg ccgtgcagca
     1681 acggttggtg ttatggcgcc caagcctcca tcatcggatg atcagattcc ggattccgcg
     1741 gaaaagccgg ttgcgccgga actggaccag aagctcgcag cgggcgccga ccgcgtcatc
     1801 ggcgacaaga ccgcaggctc gcctccgccc gatgacgaag cggaggatga cgacgaactc
     1861 gatatcgacg acgaagatga cgatgaggat ctcgtcgtct ttaccgcaaa ggaagccgcc
     1921 ggcgcgctcg cgacggtcta tgcgttcgtc aaaccatacc tgaagaacta caagaaaata
     1981 ctggcattcg tcggcctcgg cgtcctggtc gagacgctgt tcaacgtcat catgccgttg
     2041 agcctgaaat tcctgatcga cgatgcgctc ggcgaggaag atttcgaggc gctggtcaag
     2101 atactctcgg tactggcggc cgcgggtatc gtcacctcgg tcgttgcggt ctggtacgaa
     2161 cgatgggacg ccgaactcgc cgcaaccctg atttcagacg tccggt
//
LOCUS       DASWGM010000602         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_903616,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000602 DASWGM010000000
VERSION     DASWGM010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903616"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..620
                     /locus_tag="VGA15_17075"
     CDS             264..620
                     /locus_tag="VGA15_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX9589446.1"
                     /translation="MITILRIFAWLLAAAVTLATLGPPRFRPHAGLGQDGEHALAFVL
                     IGLAFGAAYPRHRLLATVMAVAGIGLLEILQLWAPGRHARLEDFVVDALATCVGLAIT
                     AGLDWAIRRSRPPNPA"
     gene            complement(687..1076)
                     /locus_tag="VGA15_17080"
     CDS             complement(687..1076)
                     /locus_tag="VGA15_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HEX9589447.1"
                     /translation="MRNRLAVSLIAASLFASGTAFAQSTTAAGAVNGARTGGEVAGPV
                     GEIVGGTVGAAVGAGLEIPNAVITSIQGERAPSVTVRERVVVGEPLPPTVELRPVPRY
                     SEYRYAVVNDRRVIVEPRTRKIIKIID"
     gene            complement(1290..>2445)
                     /locus_tag="VGA15_17085"
     CDS             complement(1290..>2445)
                     /locus_tag="VGA15_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9589448.1"
                     /translation="PASLVASALIECGEKKVVGAILIPSGYAETGNVKGQEEIQAIGQ
                     KYGIRLMGPNIYGFYYTPANLCATFCTAYDVKGSAALSSQSGGIGMAIIGFSRSAKMG
                     VSAIVGLGNKSDIDEDDLLTFFEQDDNTQIIAQHCEDLKDGRAFAEVARRVSKKKPIV
                     VLKAGRTSAGAKAASSHTGALAGNDKIYEDVFNQSGVIRARSLRDMLEFARGVPVLPT
                     PKGENVVIITGAGGSGVLLSDACIDNKLSLMTIPPDLDAAFRKFIPPFGAAGNPVDIT
                     GGEPPITYVNTVKLGLEDPRIHALILGYWHTIVTPPMVFARNMVEVKNEMKAKGIEKP
                     IVASLAGDIEVEEAADYLYQNGIVAYAYSTEIPVAVLGAKYKWARGAGLL"
BASE COUNT          433 a          774 c          752 g          486 t
ORIGIN      
        1 ctgggcggcc gacgaagagg atttcgagcg cctggtcgat cagtccctgt cggcgaccgc
       61 gccgacgctg attgcggtgc gtatcgacga caagccgggc gtcggcgcga cgcgacgcga
      121 tcccgtgcag atcagggagc gttttatgct gggcctcggc gtgcgggaag cattatgacc
      181 tgccggcctg cgttaactac gcgggcatcg caaattcgca cgaggcgggg tatccgatcg
      241 atgaatcgtg atatcccgtc ctcatgatca ccatcctgcg aatattcgcc tggcttttgg
      301 ccgctgccgt cactttagcc accttgggcc cgccccggtt ccggccgcac gccggcctcg
      361 gtcaggatgg cgagcatgcg ctcgcgttcg ttctgatcgg gctggcattc ggcgcagcct
      421 atccccggca tcgattgctc gcgacggtga tggctgtcgc cggaatcggc ttgctcgaaa
      481 tcctgcaact ctgggcgccg ggaaggcacg cgagattgga ggattttgtt gtcgatgcgc
      541 tggcgacctg tgtcggcctc gcgatcaccg ccgggctgga ctgggcgatc cggcgatcgc
      601 gcccaccaaa cccggcatga catgagataa aaaatccccg cggggacagt ccccgtgagg
      661 atcggtcgac gagtttgccg ctcgggttag tcgatgatct tgatgatctt gcgcgtccgc
      721 ggctcgacga tcacccgccg gtcattcacg acagcgtagc gatactcgct gtagcgcggc
      781 accggccgca gttcgacggt cggcggcagc ggctcaccca ccaccacccg ctcccgtacc
      841 gttaccgacg gggcacgctc accttgaatc gaagtgatca cggcatttgg gatttccagg
      901 cccgcgccga cagccgcacc taccgtgccg ccgacgatct cacccaccgg gccggcgact
      961 tcgccacctg tcctggcgcc attgacggca ccggccgcgg tggtggattg agcaaacgct
     1021 gtgccggacg cgaacagcga cgcggcaatc aacgaaacag caagacgatt cctcatcata
     1081 tcctccttgt gattgacggt ccctttcaac aattggaatg gagggatgtt ccggttccat
     1141 ttgtgcgtgt cgtcgttgcg tcagacgagt tcccaggcaa agtctggagc caagttccga
     1201 acacggtgcg ggcgcgaagc ggttccgctc aagaaaaatg ccgcccgaaa cacggacggc
     1261 attttgaaag ttttgtttgt cccggtttct tagagcaggc ccgcaccgcg cgcccatttg
     1321 tatttggcac cgagcacggc gaccgggatt tcggtcgaat aggcgtaagc cacgatgccg
     1381 ttctggtaga gatagtcggc ggcctcctcg acctcgatat cgcccgccag cgacgccacg
     1441 atcggctttt cgatgccctt ggccttcatc tcgttcttca cttcgaccat gttcctggca
     1501 aacaccatcg gcggcgtcac gatggtgtgc cagtaaccga ggatcagcgc gtggatgcgc
     1561 ggatcttcga gcccgagttt tacggtgttg acataggtga tcggcggttc gccgccggtg
     1621 atatcgacgg gattgccggc cgcgccaaac ggcgggatga atttgcggaa cgccgcatcg
     1681 agatcgggcg ggatcgtcat caaggagagt ttgttgtcga tgcaggcgtc cgacagcagc
     1741 acgcccgaac cgcccgcccc cgtgatgatg acgacgttct cgcccttcgg cgtcggcagc
     1801 accggaacgc cgcgggcgaa ctccagcatg tcgcgcagcg agcgcgcgcg gatcacaccg
     1861 gactggttga acacgtcctc atagatcttg tcgttgcccg ctagcgcgcc ggtatgcgaa
     1921 ctcgcggcct tggcgccggc cgatgtacgg cccgctttca gcaccacgat cggctttttc
     1981 ttcgacacgc gccttgcgac ttccgcaaag gcgcggccgt ctttcaagtc ctcgcaatgc
     2041 tgcgcgatga tctgggtgtt gtcgtcctgc tcgaagaacg tcaacagatc gtcctcatcg
     2101 atgtcggatt tgttgccgag accgacgatc gccgagacgc ccatcttggc cgagcgcgaa
     2161 aagccgatga tcgccatgcc gatgccgccg gattgcgacg acaaggccgc ggagcccttg
     2221 acgtcgtaag cggtgcagaa cgtcgcgcac agattggccg gggtataata gaagccgtag
     2281 atgttcggtc ccatcaggcg aataccgtat ttctggccga tcgcctggat ttcctcctgg
     2341 cccttgacgt tgccggtttc ggcgtagccc gacggaatca ggatcgctcc gacgaccttc
     2401 ttctcgccgc actcgatcag cgccgacgcc acgaggctcg ccgga
//
LOCUS       DASWGM010000603         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2430023,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000603 DASWGM010000000
VERSION     DASWGM010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430023"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGA15_17090"
     CDS             <1..220
                     /locus_tag="VGA15_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9589449.1"
                     /translation="EQVLRQCGDDLTRENIMRQAANLNFEIGVYLPGTHIKTSPTDFA
                     PLEQLQMMRFKGESWDLFGPIMSGEKNS"
     gene            complement(383..760)
                     /locus_tag="VGA15_17095"
     CDS             complement(383..760)
                     /locus_tag="VGA15_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589450.1"
                     /translation="MIGRIALVASVVLTSCMLIPVDTHAQDVPGIEICTVEKTMERRT
                     SCLQSNVDFLQKTITKLAADHQQKLDAANRQIEGLKASVVSLQKIVDDLQGAQKKTLE
                     DQKKKADAPPAKDAVPAAKDAGK"
     gene            1109..1330
                     /locus_tag="VGA15_17100"
     CDS             1109..1330
                     /locus_tag="VGA15_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589451.1"
                     /translation="MATRERRLAEFNGNGEPPPDQPVQVLCEDHSGTYQLPFACRFVG
                     GEWRNNESGGTVEATVVGWRLPRTGSRAL"
     gene            complement(1556..1990)
                     /locus_tag="VGA15_17105"
     CDS             complement(1556..1990)
                     /locus_tag="VGA15_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9589452.1"
                     /translation="MNQHAVVNKFSHVKPGDTEFTEGGLRDFFLYRDLGIAAATGGQV
                     ICHLVKANPEMPPEEGTGWHKHECDFQIVIMTKGWARFMYEDKPTLVQAGDVVHQRPG
                     IVHYLYDYSPDMEYLEIVSPADFKTVDVPPATDKVPPVTPWK"
     gene            complement(2337..>2967)
                     /locus_tag="VGA15_17110"
     CDS             complement(2337..>2967)
                     /locus_tag="VGA15_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9589453.1"
                     /translation="SKNYSSWSLRGWLLTKFSGLEFEEIITAPDDASARAEILLLSSS
                     ILVPCLRHEGAIVWDTLAIADYLNEVMPDAGLLPADRVLRAHCRSICGEIHSGFTTLR
                     ASLPVNLKGHFPGFKIWSRAQADIDRVCTIWHECFAKSGGPFLFGERRTMADAMYAPV
                     VTRFMTYDVKLEPRLKAYADMVMTLPEMLEWIEAAKAEPADIEELEVEY"
BASE COUNT          604 a          890 c          889 g          584 t
ORIGIN      
        1 ggaacaggtg ctgcggcagt gcggtgacga tctcacgcgc gaaaacatca tgaggcaggc
       61 agccaatctg aatttcgaaa tcggcgtgta tctgccgggc acgcatatca agaccagccc
      121 gaccgacttc gcgccgctcg aacagctgca gatgatgcgc ttcaagggcg agagctggga
      181 tttgttcggg ccgatcatgt caggcgaaaa gaacagctga gcttgcgtaa tccctccccc
      241 ttgtggggag ggtcggcgcg tagcgccggg gtgggggtag cgcacggagt gcgcgaaaaa
      301 tctttgcgga agcagccccc accggaccgg ccttcggccg gccaccctcc ccacgagggg
      361 gagggattaa gaaagcagag ccttacttcc cggcatcctt cgccgccggc accgcatcct
      421 tggccggcgg tgcgtcggct ttcttcttct gatcctcgag cgttttcttc tgcgctccct
      481 gcaggtcgtc cacgatcttc tgcaaggaga caacgctagc tttcaagccc tcgatctgcc
      541 ggttcgcggc atcgagtttc tgctgatgat cggcggcgag ttttgtgatc gtcttttgca
      601 ggaagtcgac attactctgc aggcaactgg tccgccgctc catggtcttt tcgacggtgc
      661 agatttcgat gccgggaacg tcctgggcat gcgtatcgac cggtatcagc atgcaggacg
      721 tcaaaaccac tgacgcgacg agagcgatgc ggccaatcat cgacatcctc actgaagcat
      781 ttttccggct aagtggaatc cgattggcgg caagaaaatg ctcctgaccc ataaattgcg
      841 cgtattttgc cgcagatcgc ttcacgcaac ctgcgtcaac ttgattgcgg agcaaaacgg
      901 tagacaccgt tttgcggaat ccgcgataag tacttctcat cacatcaaat tgcgcggagc
      961 cggtgcgcga caagaacgat accacactga aacctcattc gcgcggtgag gtttacgatt
     1021 aatacccaag ctcaggcagg gggcacggct tgagggcgcg gaacgcgaac gcggcgcttg
     1081 cgcctctctt tcagggagaa atacggaaat ggcaacgcgc gaacgacgtc tggccgaatt
     1141 caacggcaac ggtgaaccgc cgccggatca gccggttcag gtgctttgcg aggaccacag
     1201 cggcacgtat caacttccgt tcgcctgccg tttcgtcggt ggcgaatggc gcaacaacga
     1261 gtccggcggg accgtggagg cgaccgtggt cggctggcgc ctgccccgca caggcagccg
     1321 cgcgctttag gctcggccgc tgtgccataa tgcaacggag gccggcggcg ttttaagggt
     1381 gagaacgccg acggaacgaa ccgcgcccga atcagtgctt ccgccgtgta ctctcatgtt
     1441 cacggctaga tgtcggtctg aatgtcagac ggcgcaccac atccagcacc gctgcaaccg
     1501 agcgcagcca acgaccgacg acaaatgtta actcctccgg cgtcgacact tagccctact
     1561 tccagggcgt caccggcggc accttgtcgg tggccggcgg cacgtcgacg gttttgaagt
     1621 ccgccgggct gacgatttcg agatattcca tgtcgggcga gtaatcgtag agatagtgca
     1681 cgatgcccgg ccgctgatgg acaacgtcgc cggcctgaac cagggtcggc ttgtcttcgt
     1741 acatgaagcg ggcccagccc ttagtcatga tgacgatctg gaaatcgcat tcgtgtttgt
     1801 gccagccggt gccttcttcc ggcggcatct cgggattggc cttgaccaga tggcagatca
     1861 cctgcccgcc ggtcgcagcg gcgatgccaa gatcgcggta caggaagaaa tcgcgtaagc
     1921 cgccttcggt aaattcggta tcaccgggtt tgacgtggga aaacttgttg acgacagcgt
     1981 gctgattcat cagggcctcc actgttcttg ttcgagcaat cgcgacaagg cccaggcgtc
     2041 caatttgcgt ccaatttgcg tccaattttt tggcagcgcg ggattgccgt tttcgcggct
     2101 tcattttgct atgcgaaaat cgcgccagcc gcgctgcaac gcgacgaatt cagcggccac
     2161 cgcggcgagg atgccgaagc tcttggacgg aaccggaaat ccgggtcgag cgatttttgg
     2221 cgcaccactt ccgggccggt catgccgatt gccgaaacgc ccgggatggc accaagttaa
     2281 gcaaatcctg cccgatgctt aagcccctca aacaggcagc gaggggcctg cgttgcctaa
     2341 tattcgacct caagctcttc gatatcggcg ggctcggcct tggcggcctc gatccattcc
     2401 agcatctcag gcaacgtcat caccatgtcg gcataggctt tgagtctcgg ctcgagcttc
     2461 acatcatagg tcatgaagcg ggtcacgacc ggcgcataca tggcgtccgc catggtccgg
     2521 cgttcgccga acaggaacgg gccgcccgat ttggcaaagc attcgtgcca gatcgtgcag
     2581 acccgatcga tgtcggcttg cgcgcgcgac cagatcttga agcccgggaa atggcctttc
     2641 agattgaccg gcagggacgc gcgcagggtg gtaaatcccg aatggatttc gccgcaaatc
     2701 gaacggcaat gcgcccgcaa gacgcggtcc gccggcaaca ggccggcgtc cggcatgacc
     2761 tcgttgaggt aatccgcgat cgccagcgta tcccacacga tggcgccctc gtgccgcaga
     2821 catggtacca ggatcgatga cgacagcagc aggatttcgg ctcgcgccga agcatcgtcc
     2881 ggcgcggtga tgatttcctc gaactccagc ccggagaact tcgtcagcag ccagccgcgc
     2941 aacgaccacg acgaatagtt cttgctg
//
LOCUS       DASWGM010000604         6082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1862418,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000604 DASWGM010000000
VERSION     DASWGM010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862418"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1372
                     /locus_tag="VGA15_17115"
     CDS             161..1372
                     /locus_tag="VGA15_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9589454.1"
                     /translation="MTDSATSHLLPVFARVDLGFQRGEGAWLIATNGERYLDFTSGVA
                     VNALGHAHPHLVAALQEQATKLWHMSNLFKSPDGERLAARLCEASFADLVFFANSGAE
                     AMECAIKITRKHHAAKGHPERYRIITFDGAFHGRTLATLAATGSAKYLEGFGPPLDGF
                     DQVTLGDLDAVRRAIGPHTAGILIEPLQGEGGVRAAPHVFFKALRQLCDDNGLLLIFD
                     EVQTGMGRTGDLFAYQRIGVTPDVMPLAKALGGGFPIGACLATAEAAAGMTPGSHGST
                     FGGNPLAVAAANAVLDVMQKPGFFEHVRKMSLLLKQKLASVVDRHPKVLAEVRGEGLL
                     IGLKAVVPSVDLVTALRAQKLLTVGAGENVVRFLPPLIVTEAEIEESVGCLERACAAL
                     SSDQLKRAAGK"
     gene            1369..2295
                     /gene="argF"
                     /locus_tag="VGA15_17120"
     CDS             1369..2295
                     /gene="argF"
                     /locus_tag="VGA15_17120"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX9589455.1"
                     /translation="MSKALRHFLDLSELPTKELRNMLALSVAMKAKLKAHAEPVKPLE
                     GKTLAMIFEKPSTRTRVSFDVGMRQLGGEAIMLTGAEMQLGRGETIADTARVLSRYVD
                     AIMIRILNHDALLELAAHATVPVINGLTRRSHPCQVMADLMTFEEHRGPIEGRTIAWT
                     GDDNNVLASWAHAAERFRFKLNVATPPQLAPNKSMRDWIKATQAPIVLGNDPEAAVRG
                     ADCVVTDTWFSMGDKDGEHRHNLLKPYQVNAKLMSLAKPDALFMHCLPAHRGDEVTDE
                     VIDGPQSVVFDEAENRLHAQKGILAWCFEAVA"
     gene            2511..3515
                     /locus_tag="VGA15_17125"
     CDS             2511..3515
                     /locus_tag="VGA15_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HEX9589456.1"
                     /translation="MTSQSPDTKIFLETPVRAPSAVPIDDTVLPFEVASLDLRGRLTR
                     LGPALDDVLARHDYPPAVGKLLGEAIVLTTLLGSSLKFEGRFILQTQTDGPVSFLIVD
                     FQAPDRLRAYARFDAKRLKDGLDSGALLGHGHLAMTIDQGPDMSRYQGLVALEGGSLE
                     DAAHEYFLRSEQIPTRVRLAVGEEWRGGEEGPRHRWRAGGMLLQFLPKAPERARQADL
                     HPGDAPEGVVTHTVDEDDAWIEGQSLIGTVEDVELIDPDLSGERLLYRLFHERGVRVF
                     PPLPLRAQCSCSRDAVSSMLASFAAKDRADMVKDGKVVVTCEFCSSVYEFTPQEAGVE
                     "
     gene            3786..4625
                     /locus_tag="VGA15_17130"
     CDS             3786..4625
                     /locus_tag="VGA15_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589457.1"
                     /translation="MDPVFITRTIEATPFPSAPNVVISYPHREDWWHRYPALRKAGGR
                     NRNIDEFQWLYQDSKRAYQDSVLKRLRKIQHWATGRAVLAELRARPSYSVYTFPWDFL
                     PSIDLGDPRDLGMTETLRIPQTRRERARGIKPPGTKFRVRGVCYASMDKPGAVDVFYS
                     DYRCEESDADGTLLHELVHAMRIISGVDRGVPMGGGYENSEEFYANTVEMIYRSERGQ
                     DVFDYAFHPINQASVLKQPKARALLTDLRYAQPSLFLALARVDAPFNPIRPIADQLLR
                     IDL"
BASE COUNT         1158 a         1835 c         1924 g         1165 t
ORIGIN      
        1 atgggttcca ttgccaggtt gacagctatc gccacccgca tagaatgcgt ctcacgtgcc
       61 gccccgaaaa ggcggcacgt tttgttttcc agggctggct gttgatgcga tcgcgccaat
      121 gcgcgcgtcg gggccggcgc caaaaccttc agtgatcaac atgaccgaca gcgcgacgtc
      181 gcatctgctc cccgtattcg ccagggtcga tctcggtttc cagcgcggcg aaggcgcatg
      241 gctgatagcg accaacggcg aacgctatct cgatttcacc tctggcgtcg cggtgaatgc
      301 gctgggccac gcgcatccgc atctggtcgc agcgttgcag gaacaggcga cgaaactgtg
      361 gcacatgtcg aacctgttca agagtccgga tggcgagcgg ctcgcggcac ggctgtgcga
      421 agcgagcttt gccgacttgg tgtttttcgc gaactccggc gccgaggcga tggaatgcgc
      481 gatcaagatc acgcgcaagc atcacgccgc caaaggccac ccggagcgct atcgcatcat
      541 caccttcgac ggcgcgtttc acgggcgaac gctggcgacg ctggccgcga ccgggtccgc
      601 caaatatctc gagggttttg ggccgccgct ggatggcttc gatcaggtga cgctcggcga
      661 cctcgacgcc gtcagacgcg cgatcggtcc gcacaccgcg ggcatcctga tcgaaccgct
      721 gcagggcgag ggcggcgtac gcgcggcgcc gcacgtcttc ttcaaggcgt tgcgccagct
      781 atgcgacgac aacggcctgc tgttgatctt cgatgaggtg cagaccggca tgggccgcac
      841 cggcgacctg tttgcttatc aacgcatcgg cgttacgcct gatgtgatgc cgctggcgaa
      901 agcgctcggc ggtggtttcc cgatcggtgc ctgcctggcg acggctgagg ctgccgccgg
      961 catgacgccg ggttcgcacg gctcgacctt cggcggcaat ccgctggcgg tggcggcggc
     1021 caatgcggtg ctggatgtca tgcagaagcc cggcttcttc gagcacgtgc ggaagatgtc
     1081 gctgctgctc aagcagaagc tggcctcggt ggtcgatcgc cacccgaagg tgctcgccga
     1141 agtgcgcggc gaagggctgc tgatcggtct gaaggcggtg gtgccgtcgg tcgatctggt
     1201 gacggcgttg cgcgcgcaaa agctgctcac cgtcggcgcc ggcgaaaacg tggtgcggtt
     1261 cctgccgccg ttgattgtga ccgaggcgga gatcgaggaa tccgttggat gtcttgagcg
     1321 cgcctgcgca gcactttcca gcgatcagtt gaaacgggcg gcggggaaat gagtaaagcg
     1381 ctgcggcatt ttctcgatct cagcgagctt ccgaccaaag agttgcggaa catgctcgcc
     1441 ttgagcgtcg ccatgaaggc gaaattgaag gctcacgccg aaccggtcaa gccgctcgaa
     1501 ggcaagacgc tggcgatgat tttcgaaaag ccctcgaccc gcacgagggt gtcgttcgac
     1561 gtcggcatgc gccagctcgg cggtgaagcg atcatgttga ccggcgcgga aatgcagctc
     1621 ggccgcggcg agacgattgc cgacaccgcg cgggtgctgt cgcgctacgt cgacgccatc
     1681 atgatccgca ttctcaacca tgatgcgctg ctggaactcg ccgcgcacgc caccgtgccc
     1741 gtcatcaacg gcctgacgcg gcggtcgcat ccctgccagg tgatggctga cctgatgacc
     1801 ttcgaagagc atcgcggccc gatcgaaggc cgcaccattg cctggaccgg cgacgacaac
     1861 aacgtgctgg cgtcgtgggc gcatgcggcg gaacggtttc gattcaagtt gaacgtggcg
     1921 accccgccgc agctcgcacc gaacaaatcg atgcgggact ggatcaaggc gacccaggcg
     1981 ccgatcgtgc tcggcaacga tccggaagca gcggtccgcg gcgccgattg cgtggtcacc
     2041 gacacctggt tctcgatggg cgacaaggac ggcgagcacc gccacaacct cctgaaaccg
     2101 taccaggtca acgccaagct gatgtcgctg gcaaaacccg acgcgctgtt catgcattgc
     2161 ctgcccgcgc atcgcggcga cgaagtcacc gacgaggtga tcgacggccc gcaatcggtc
     2221 gtgttcgacg aagccgaaaa tcgcctgcac gcgcaaaagg gcattctggc gtggtgtttt
     2281 gaggcggtgg cgtgagcccc ggagtttggc aatcataata tcgtcgtccc ggcgaacgcc
     2341 gggacccata cggcgcggac tcacgtcatg cgcagtgtgg tcgacgcctt ctgcaacaat
     2401 tgacgccagg gattatgggt cccggcgttc gccgggacga cggacggagc ggtattccat
     2461 ctaactcccg accctttcga tttgcatctc gcgaccccgg ataaaccgcc atgacctcac
     2521 aatcccccga tacgaaaatc ttccttgaaa ccccggtccg cgcaccgtcg gccgttccga
     2581 tcgacgatac ggtgctgccg ttcgaggttg cctcgctcga tctgcgcggc cgcctgaccc
     2641 ggctcgggcc cgcgctcgac gacgtcctgg ccaggcacga ttatccgccg gcggtcggca
     2701 agctgctcgg cgaggcgatc gtgctcacga ccttgcttgg ctcctcgctg aaattcgagg
     2761 gccggttcat tctgcagacc cagaccgacg gcccggtgtc gtttctgatc gtggacttcc
     2821 aggcgccgga tcgcttgcgt gcttacgcgc gcttcgacgc gaagcgcctg aaggacgggc
     2881 ttgattccgg cgcgctattg ggacacggcc atctcgccat gaccatcgat cagggccccg
     2941 acatgagccg ctatcagggg ctggtggcgc tggaaggcgg cagcctggaa gacgccgcgc
     3001 atgaatattt tctgcgctcc gaacagatcc cgacccgggt gcggcttgcg gtcggcgagg
     3061 aatggcgggg cggcgaagag ggtccaaggc atcgctggcg cgctggtggc atgctgctgc
     3121 aatttttgcc gaaggcgccg gagcgggcgc ggcaggccga tctgcatccc ggcgatgcgc
     3181 cggagggcgt ggtcacccac acggtggacg aagacgacgc ctggatcgaa ggccagtcgc
     3241 tgatcggaac cgtcgaagat gtcgaactga tcgatccgga tctctccggc gagcggctgt
     3301 tgtatcgcct gtttcacgag cgcggcgtcc gggtgtttcc cccgctgccg ctgcgcgcgc
     3361 aatgttcctg ctcgcgcgac gcggtgtcgt cgatgctcgc gagcttcgca gcaaaggatc
     3421 gcgccgacat ggtgaaggac ggcaaggtgg tcgtcacctg cgagttctgc agctcggtct
     3481 acgaattcac gccgcaggaa gccggcgtgg agtagggcgg gcacatctag tcgtcatgcc
     3541 cggacttgat ccgggcatcc atcaatcttc gtgaaaagtt gtttcgaaga agatggatca
     3601 ccggggcaag cccggcgatg acgatcgggt tgcccaaggc ttcttcgcaa gaagagatgt
     3661 catccgatcg taggacgtcg ctgcggtgcg ccggcagtat gactttgcag atactccgat
     3721 gtgattgaac ccgttcaggg tatggggcga ccacctccgg acccggcttg cgcaggagat
     3781 cgcccttgga tcccgttttc atcacccgaa caatcgaggc gacgccattc ccgtcggcgc
     3841 cgaacgtcgt catttcttat ccgcaccgcg aagattggtg gcatcggtat ccggcgctgc
     3901 ggaaggcggg cgggcgaaac cgcaatattg atgaattcca atggctttat caggactcga
     3961 agcgcgctta ccaggacagc gtgctcaagc gacttcgcaa aatacagcat tgggcaacgg
     4021 gccgggcggt ccttgcggaa ctgcgcgcgc ggccctccta ttcggtttac actttcccgt
     4081 gggacttctt gccgtccata gacttggggg acccacgcga cctgggaatg actgaaacgc
     4141 ttcgaattcc gcaaacgcgg agggaacgtg cccgtggaat caagccgcca gggacgaagt
     4201 tccgcgtgag gggggtgtgc tacgccagca tggataagcc gggcgccgtc gacgtgttct
     4261 attcggatta ccgttgcgaa gagagcgacg cggatgggac gctgttgcac gagctcgtgc
     4321 atgcgatgcg cataatatcg ggcgttgacc gaggtgtccc gatgggcgga ggttacgaga
     4381 acagcgaaga attttacgcc aatacggtcg agatgatcta tcgatccgag cgaggacagg
     4441 acgtcttcga ctatgcgttt caccctatca atcaggcatc ggttctcaag cagcccaagg
     4501 cgcgcgcgtt gctcactgac cttcgatacg cgcagccgtc gctctttctt gcgctggcga
     4561 gagtggatgc acctttcaac ccgataaggc cgatcgctga ccagctgttg agaattgact
     4621 tgtaattgag cgagtcattg acgttgcggc tactcatcag gggccggctt gcgctggaga
     4681 tcgaccttgg ctgttcgatc ctgtttcgat caccagaaaa atcgcagcgc cgcccttttc
     4741 gcaagccccc aatgtcgtca ttgcctatcc gcacttcgag gattggaaga aactgtgtaa
     4801 gccgctgccg ctgcgcatgc cggacgcgca aggcatctac cgcaatgtcg atgcgttcga
     4861 gtcggcgacg aagtgccttt gggcgatgtc gatacagtcg ccgacattga aatcgccgtc
     4921 acatcgataa gcgagggtcg gtcatttcgg ctgccggcgc cgctctcgat cagacttact
     4981 ggaaagccgt tccggtcgtt tccccctttc cccttgccta ttacgaatgg ctcggcgtta
     5041 agaacacggc cgactcgttc aagcaactcg gtggctcggt tctctcgttt tcaactcata
     5101 tttcaaaccg tctcgccgcc tacactgcca tgttaaacca gttggacggc gaattggtaa
     5161 aggtgagcaa tgaaatgggg aagtacactt gagacccggg tatcaacgat aactcaacga
     5221 ggccgccaca gcggaaattg aatttgaggg cacccgctcg cgttgacgta gcgcaacggt
     5281 cgcgggcaca tcacttcggc ttccactcgc gccgtccgca gtcgcgcgcc tgtcgagccg
     5341 cggctgtttg aaaattgaat caatgcaatt cattgaatac gaacacacct cactcccaac
     5401 cgtcatcgcc gccacgccac cgggtcggcg cagcgcgccg cccgatgaca ggctccatcg
     5461 gtgatccagt attccagaga cgccggtaat gaaccgaaag gccgcggcgt aatggatacg
     5521 ccgctttcgc ggagtatggc ggagtttgga gagcgccccc tggttcagcg tccgtttggc
     5581 ctcgtgcccg acgggcaaat cagtccgtcc attgattttc ctgtccagcc ccctttgcaa
     5641 aaatattcct gtttccgcac cccccaaatc acctctagaa ctttgcacat cccgtcccac
     5701 acagaggggc gtatcatgat cgtcacgaac gtaggggcgg gatgcggtgg acgcggcagc
     5761 gttttgcgcg cgatgggatt gcagggcgac tcgaaaggct tgtgagcgat caaagagcgt
     5821 gcggacgaga gatgttgctg cggacggcaa aatcgtgtgg tcctgacgcc ccgacgctgg
     5881 cgtcaagttt tgcgggatgg caagtcggcc caaccgggcg cggacaagtc agatatccgc
     5941 ggatgacggt ggcaaaagag cccggtcacc ggggagagca cgacataagc cgttaaaacc
     6001 attgcgtgcg ggaatgccgg gtgttccggt gcgaccgtgg tgaagactcg tgtgctttct
     6061 acactacgca cacgaggctg cg
//
LOCUS       DASWGM010000605         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2011251,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000605 DASWGM010000000
VERSION     DASWGM010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011251"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VGA15_17135"
     CDS             complement(<1..548)
                     /locus_tag="VGA15_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEX9589458.1"
                     /translation="MPDMTLAPRRRPLWRLFIMPVLLLVAAAAWSAFWFFAASQVEVR
                     ADAWRAQEAKSGRVYDCAKRSVAGFPFRLEVRCDGASVSLVSQTAGQAATQTPMTAQL
                     GEILIVAQVYDPKLLIAEFTAPATIADRGQQPSMIVNWSKARSSVVGLPAIPQRASIV
                     FDDPSIVRLDGSAQAPLAGAKH"
     gene            678..1256
                     /locus_tag="VGA15_17140"
     CDS             678..1256
                     /locus_tag="VGA15_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX9589459.1"
                     /translation="MPAKIAPEAELSKGDLWVFGYGSLMWRPGFEFIEQVPARLIGEH
                     RALCVYSFDHRGTPEKPGLVLGLDRGGACRGVAFRVAAKQRSDTIDYLRSREQTTHVY
                     REVMRSVWLEDEARQRVSALAYVVDRGHVQYAGRLSLTEQVRYVRQGHGRSGNNRDYV
                     LSTVKSIEAQGFRDPQLHQLAALLHDDTPLHR"
     gene            complement(1564..>2204)
                     /locus_tag="VGA15_17145"
     CDS             complement(1564..>2204)
                     /locus_tag="VGA15_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9589460.1"
                     /translation="RSSVWLMRVICNTKVEYRGLEKIPKGPLIVASKHQSMWETFALM
                     QFFDAPLFIYKRELAWIPFFGWYLMKSKMIGVDRGGGVRSLMQMARRAPKEIRSGRQL
                     IIFPEGTRRPVDAPPDYMTGVGQIYVNSGVPCLPVALNSGLFWPRRTFMRYPGTLVVE
                     FLDPLPSGLTRKDFIARIREAIEDATNRLVAAARQEQAQLFGRVPGSTPVKA"
BASE COUNT          409 a          712 c          684 g          399 t
ORIGIN      
        1 atgtgttttg cgcccgcaag cggcgcctgc gccgatccat cgaggcgaac gatcgaggga
       61 tcgtcgaaca cgatcgaggc gcgctgcgga atcgccggca gtcccaccac gctgctgcgc
      121 gccttgctcc agttcacgat catcgatggt tgctggccgc gatcggcgat cgtcgccggc
      181 gcggtgaatt ccgcgatcag caatttggga tcgtagacct gggccacgat gaggatttcg
      241 ccaagctgcg cggtcatggg cgtctgcgtc gccgcctgcc ccgcggtctg cgacaccagc
      301 gacacgctgg cgccatcgca gcggacttcg aggcggaacg ggaagccggc caccgagcgt
      361 ttggcgcaat catagacccg gccggatttc gcctcctgtg cgcgccaggc atcggccctt
      421 acctcgactt gggatgcggc aaagaaccag aatgcgctcc atgccgcggc agcgacaagc
      481 agcaatacgg gcatgatgaa gaggcgccaa agcgggcgcc ggcgcggcgc gagggtcata
      541 tcgggcatta cggtgacctt tgggcaatga ttttgtcaat aaagcgatgc acacccgcca
      601 caatcaagtt accggatgtt cccgaatcat ggaattccgc ttcgcctgaa aaccttcttc
      661 tgatagccgt tcgcgcaatg ccagcgaaaa tagcccccga agccgaattg tccaaaggcg
      721 acctctgggt gttcggctat ggctcgctga tgtggcggcc gggcttcgaa ttcatcgagc
      781 aggttccggc gcggctgatc ggcgagcatc gcgcgctctg cgtctattcg ttcgaccatc
      841 gcggcacgcc ggaaaagccc ggccttgtgc tcgggctcga ccgcggcggc gcctgccgcg
      901 gcgttgcatt ccgcgtcgcg gcgaagcaac gcagcgatac catcgactac ctgcgcagcc
      961 gcgagcagac cacccacgtc tatcgcgaag tgatgcgttc ggtctggctc gaagacgagg
     1021 cccgccaacg ggtcagcgcg ctggcctatg tggtcgatcg cggccacgtc caatatgccg
     1081 gaaggctctc gttgaccgaa caggtgcgct acgtgcggca ggggcacggc cgttccggaa
     1141 acaatcgcga ctacgtgctg tccaccgtga agtcgatcga ggcgcaaggt tttcgcgacc
     1201 cccaattgca tcagcttgcc gcgctgctgc atgacgacac gccgctgcat cggtgacttt
     1261 gccctcccct gccgggaggg ctatcgcata tggaattttt cgcctggcgt ttcaacgagt
     1321 cgtccctgcg cacgcaggga ccccagcgtg agcgcaattg cgctcatcgc ggcgccgcgg
     1381 actctcggtt cggtactgga gcagaggcct tttttcactt ttgacgccag gggtgatggg
     1441 tccctgcgtt cgcagggacg acctgctgag ggtttgttcg aggccatgac tgcatcaacg
     1501 cacggggcaa ttcaatcata agcgatagcc ctccccccgc agggagggtg agcgagaact
     1561 gccctatgcc ttcaccggcg tcgaacccgg cacgcggccg aacaactgcg cctgctcctg
     1621 ccgcgccgcc gcgaccagcc ggttggtcgc atcctcgatc gcctccctga tccgcgcgat
     1681 gaaatccttg cgggtaaggc ccgacggcag cggatcgagg aattcgacca ccagcgtgcc
     1741 gggatagcgc atgaaagtcc ggcgcggcca gaacagtccg gaattcagcg cgaccggaag
     1801 gcagggcacg ccgctgttga cgtagatctg cccgacgccg gtcatatagt ccggcggcgc
     1861 gtcgaccggc ctgcgggtgc cttcgggaaa gatgatcagc tgtcggccgc tgcgaatctc
     1921 ctttggcgca cgccgcgcca tctgcatcag cgatcgcacg ccgccgccgc gatcaacgcc
     1981 gatcatcttc gacttcatca gataccagcc gaaaaaggga atccaggcca gttcgcgctt
     2041 gtagatgaac agcggcgcat cgaagaactg catcagcgcg aaggtttccc acatcgactg
     2101 atgcttcgac gcgacgatca ggggcccttt cggaattttc tccagcccgc gatattccac
     2161 cttggtgttg cagatcacgc gcatcagcca gacgctgctg cgcg
//
LOCUS       DASWGM010000606         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_743461,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000606 DASWGM010000000
VERSION     DASWGM010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743461"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1597
                     /gene="ahcY"
                     /locus_tag="VGA15_17150"
     CDS             167..1597
                     /gene="ahcY"
                     /locus_tag="VGA15_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX9589461.1"
                     /translation="MTATAKKPAFTEYIVKDISLAEFGRKEISLAETEMPGLMATREE
                     YGPTQPLKGARIAGSLHMTIQTAVLIETLAALGADIRWVSCNIYSTQDHAAAAIAAAG
                     IPVFAVKGESLTEYWDYTAKLFDWHGGGTPNLILDDGGDATMFVHQGLRAERGDTVFL
                     DQHNSEEEEVFYALIKRILKEKPKGWFAEIAKNIRGVSEETTTGVHRLYDMEKAGTLL
                     FPAINVNDSVTKSKFDNLYGCRESLVDGIRRGTDVMMSGKVAMVAGFGDVGKGSAASL
                     RQAGCRVMVSEVDPICALQAAMEGYEVVTMEDAAPRADIFVTATGNKDIITIEHMRAM
                     KDRAIVCNIGHFDNEIQIAHLKNLKWNNIKPQVDEIEFPDGKRMILLSEGRLVNLGNA
                     MGHPSFVMSASFTNQTLAQIELFANNKGGKYEKKVYVLPKSLDEKVARLHLAKIGVKL
                     TKLRPDQADYIGVKPEGPFKADHYRY"
     gene            1811..>2052
                     /locus_tag="VGA15_17155"
     CDS             1811..>2052
                     /locus_tag="VGA15_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX9589462.1"
                     /translation="MQNEHVDVLIVGAGLSGVGAGYHLQHKCPAKSFVILEGRETIGG
                     TWDLFRYPGVRSDSDMFTLGYSFKPWTEPKAIADGPR"
BASE COUNT          447 a          629 c          617 g          359 t
ORIGIN      
        1 gaggtgcgcg cactttcttg cgcgcctcga aggatgagcc gcacgcactt acttatcatc
       61 cttcgaggct cgccgaagcc ggctcgcacc tcaggatgac gccacaaagc gctcagtttc
      121 gtttcgtacc gataagtccg aattcatccg ttcaggaacc catcacatga ccgccaccgc
      181 caagaagccc gccttcaccg agtacatcgt caaggacatc tcgctcgccg aattcggccg
      241 caaggaaatc tcactggccg agaccgagat gcccggcctg atggcaacgc gcgaggaata
      301 cggcccgacc caaccgctga agggcgcgcg cattgccggc tccctgcaca tgacgatcca
      361 gaccgcggtc ctgatcgaga cgctggcggc gctgggcgcc gacatccgct gggtttcctg
      421 caacatctat tcgacccagg atcacgccgc agccgcgatc gccgcggccg gcattcccgt
      481 gttcgcggtg aagggcgaaa gcctcaccga atattgggac tacaccgcaa aattgttcga
      541 ctggcacggc ggcggcacgc ccaacttgat cctcgacgat ggcggcgacg ccaccatgtt
      601 cgtgcatcag ggcctgcgcg ccgaacgcgg cgataccgta ttcctcgatc agcacaacag
      661 cgaagaggaa gaggttttct acgccctgat caagcggatc ttgaaggaga aaccgaaggg
      721 ctggttcgcc gagatcgcca agaacatcag gggcgtttcg gaagagacga cgacgggcgt
      781 gcaccggctg tatgacatgg agaaagcggg cacgctgttg ttcccggcga tcaatgtcaa
      841 cgactccgtg acgaaatcga aattcgacaa cctctatggc tgccgtgaat cgctggtcga
      901 cggcatccgc cgcggcaccg acgtgatgat gtcgggcaag gtggcgatgg tcgcgggctt
      961 tggcgacgtc ggcaagggct ctgcggcttc gctgcgccag gccggctgcc gagtcatggt
     1021 gtcggaagtc gatccgatct gcgcgctgca ggcggcgatg gagggctacg aagtcgtcac
     1081 catggaggac gccgcaccgc gtgccgacat cttcgttacc gcgaccggca acaaggacat
     1141 catcaccatc gagcacatgc gcgcgatgaa ggaccgcgcc atcgtctgca acatcggcca
     1201 tttcgacaac gagatccaga tcgcgcatct gaagaacctg aagtggaaca acatcaagcc
     1261 gcaggtcgac gagatcgagt tccccgacgg caagcgcatg atcctgttat cggaaggccg
     1321 cctggtgaac ctcggcaacg cgatgggaca tccgagtttt gtcatgtccg catcgttcac
     1381 caaccagacg ctggcgcaga tcgaattgtt cgccaacaac aaaggcggca agtacgaaaa
     1441 gaaggtctac gtgctgccga agtcgctgga cgagaaggtg gctcggctgc atctggccaa
     1501 gatcggcgtg aaactcacaa agctgcgccc ggaccaggcc gactatatcg gcgttaagcc
     1561 ggaaggcccg ttcaaggcgg atcattacag gtattgattt caccacccgt cattgccggg
     1621 cttgacccgg caatccatcc ttcaaagaag atggatacgc gggtcaagcc cgcgtatgac
     1681 agcacgaaag ccccggagcg atccggggtt tttcgtttgc gcgcggttgc attttacctc
     1741 ggcagcgcac taactagcct aaatggacgg caagccgaat tgcccgacaa aatccgccgg
     1801 ggaggccacg atgcaaaacg aacatgtcga cgtgctgatc gtcggtgccg ggctgtccgg
     1861 cgtcggcgcg ggctatcacc tgcagcacaa atgtccggcc aagagttttg tcatcctcga
     1921 gggccgcgag acgatcggcg gcacctggga tttgttccgc tatcccggcg ttcgctccga
     1981 tagcgacatg ttcacgctgg gctattcgtt caagccctgg accgagccga aggcgatcgc
     2041 ggacgggccg cg
//
LOCUS       DASWGM010000607         5165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_461698,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000607 DASWGM010000000
VERSION     DASWGM010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461698"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..353)
                     /locus_tag="VGA15_17160"
     CDS             complement(93..353)
                     /locus_tag="VGA15_17160"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9589463.1"
                     /translation="MALVEIYEAKRWPIEIDETFDPINVLRYAIDELGHTQAELAELL
                     GSRSRASEVLSRRRALTVEMIHKVGEAWKIPADLLASSSLQD"
     gene            complement(442..597)
                     /locus_tag="VGA15_17165"
     CDS             complement(442..597)
                     /locus_tag="VGA15_17165"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HEX9589464.1"
                     /translation="MLNRERVRFEVAGGNYRLVAAFDFRRQIAFVKLIGTHAEYDRID
                     ALKVSQF"
     gene            909..1166
                     /locus_tag="VGA15_17170"
     CDS             909..1166
                     /locus_tag="VGA15_17170"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9589465.1"
                     /translation="MVNLILRKGAEEARNQLPDLLEAAEKGRSTIITRHGRPVAALVP
                     IEAYGAAIRQQPLMPVAGSGRGLWGKNSTRTLRKLRDEWSR"
     gene            1154..1579
                     /locus_tag="VGA15_17175"
     CDS             1154..1579
                     /locus_tag="VGA15_17175"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX9589466.1"
                     /translation="MEPLDFRNLPEDALVLIDSAPIIYFLEGHTKFGPRFKPLFEAHG
                     TGRLRFAVTTITIAEVLTGPLQAGDDALVQRYRAILESWQPIALDVDIAESAARLRAS
                     LRLKLADAVQAASALAINAAALVTHDRDFSRVRSLRLIS"
     gene            1576..3903
                     /locus_tag="VGA15_17180"
     CDS             1576..3903
                     /locus_tag="VGA15_17180"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX9589467.1"
                     /translation="MKAQHIDVTIVHETLSSLRGVAMSSIGTKLLNSLRSIERPGDFC
                     VGGIREIVMPTIDIDGVGRIAFPILPVQAERLAAIAEAAPYGRGEKTVVDREVRRTWQ
                     VDSAKVRIGGRHWDKTLASLVADIALGLGVSEPVAAEFYKLLVYDTGSFFVDHRDTEK
                     VPGMFATMVLVLPSTHSGGELVIKHLGREVVLDARPEEPSEIGFAAFYADCVHEVRPV
                     KTGCRLTLVYNLCFVGERRPLKAPDYRAEHGRVVELLRSWASAEDEPDKLILPLEHVY
                     TPAELSFSTLKGADAGVASVLVKAAAEADCDLHLALVSIEESGSAEHTGYYGRRRWSR
                     DEEEDEEFEVAEVIDRALILSEWRHPDGGEAGFDVFPFAEDELCPPDAFEDLTPDEEH
                     FHEATGNEGASFERTYRRAGFVLWPAARRLAVLNQAGLRTTLPYLEDLTARWEAGNAS
                     IRSPFWREADELSGHMLRSWSRSPWRADDDEEAGRMLDLQVRLGNVERIDAFLAELSA
                     EGHYAAHDNGAIVCAAALLPATRAAELLVRIVRRNAPARLGACGDLLLRCVAAPMGTT
                     GDLGQISVALIDALPGDPTRREEVGTWTRPTPVTPGFVVDLLTATSRIDAGLAARAIE
                     HLLAWPKTYSPDDVLVPAARMFAKLAESTAWPAVGQLREASLDHLRRRIALPLEAPRD
                     WARANPLECTCGDCRALGAFLVVPDQQQWRLKAVQDRRTHVEQSLERVACDLNLTTER
                     RGSPHTLVATKNQASYERRAEQRRQDLEHVSALGE"
     gene            4270..4572
                     /locus_tag="VGA15_17185"
     CDS             4270..4572
                     /locus_tag="VGA15_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589468.1"
                     /translation="MASKSQRKATRTHRRRAAARGFVRVEVQAPKKDAGLIRALAETL
                     RGGAEKAEALRSTLAKAVMNPEMGTAFDIFGSELPDEVFTGVFDQPRQRGWRRVDL"
     gene            4569..4988
                     /locus_tag="VGA15_17190"
     CDS             4569..4988
                     /locus_tag="VGA15_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9589469.1"
                     /translation="MKFLLDTNVISEIRKRGRAHPNVVHWVTRTPVEDIGTSVLVLAE
                     IRHGIELKRRSDPEQAESLDRWFLQMRTRLGGRVLPIDEPVAEAWALLGIPNPLPLID
                     GLLAATAKVHGLTFVTRNIADIVVTGVPLLDPFSGVS"
BASE COUNT          924 a         1571 c         1666 g         1004 t
ORIGIN      
        1 ctacgccaga gcacgctatg acggtcgccc ggtatccagc tgcgcgaagc cgttctacga
       61 tgattgctga aggtacgggt caggcggctc gctcagtctt gtagggacga actagctaga
      121 aggtcagccg gaatcttcca cgcttcgccg accttgtgaa tcatctcgac cgtgagcgct
      181 cgccgacgcg aaagaacctc cgacgcacgg gagcgcgagc ccagaagttc tgccaattcg
      241 gcctgggtat ggccgagttc gtctatagca taccggagca cgttgatagg atcgaaggtc
      301 tcgtcgatct cgatcggcca tcgctttgcc tcgtagattt cgacgagcgc catccggtgc
      361 gccccaacag gcctcgatcg ctgcgagcgc tgcgcggtga tcctggtctg tgcgaatggg
      421 ccggatttcc atgtcgtctc cttagaactg cgacaccttc aatgcatcga tgcgatcata
      481 ttccgcgtgg gtgccgatga gcttcacgaa ggcgatctgc cgacggaaat cgaaggcggc
      541 caccaagcga tagtttcctc ctgcgacctc aaacctcaca cgctcccggt tcagcacctt
      601 ggccttcgga gccggagcgc ctcagtgaag gtccgaaagg cgctggcggc acggctcgga
      661 agtcaggtta acgccgagcc gtcaaatatg agctttcact gcgtttgcgg taacgccatg
      721 tcttcgacga cgattgctga gccgaaaaat gctcggcatc cgagtctcgt aggctgccga
      781 tgcaaccaag aggaggcctt ttcgttcgct cggtgctttc cgttaacctg gattccgtgc
      841 cgtggcgctg gcggtgatga tattgactga ttctgtacgg tgctgtacgg ttgtttccgg
      901 agacagtcat ggtgaacctc atcttgcgta agggcgcgga agaagctcgc aatcagctcc
      961 cggatcttct cgaggcggcc gagaagggcc gttcgacgat catcaccaga catggccgtc
     1021 cggttgcagc gttggtgccg atcgaggcgt atggcgccgc cattcgccag cagccgctga
     1081 tgcccgtcgc aggatccggt cgcgggttat ggggaaagaa tagtacccgt acgcttcgaa
     1141 agctgcgtga cgaatggagc cgctagattt tcggaatctg ccggaggatg cgctggttct
     1201 catcgattcg gcacccatca tctacttcct cgaggggcac acgaaattcg gaccgcgctt
     1261 caagccgctc ttcgaagccc atggaactgg gcgcctgcgc ttcgccgtaa cgaccatcac
     1321 tatcgccgag gtgctgaccg ggcccctcca ggcaggtgat gacgcgctcg tgcagcgtta
     1381 ccgtgcgatc ctcgaatcgt ggcaaccgat cgcgcttgac gtcgacattg cggaaagcgc
     1441 tgcgagactg cgggcgtcgc tccgcctcaa gcttgcagac gcggttcaag cggccagcgc
     1501 attggcgatc aacgcggccg ccctggtgac gcatgatcgt gatttctcac gcgtgcggtc
     1561 gctgcgttta atttcttgaa ggcccagcac atcgacgtga cgatcgttca cgaaacgctg
     1621 tccagcttgc ggggagttgc catgtcgtcg atcggtacca agctgctcaa cagccttcgt
     1681 tccattgaac gtccgggcga cttttgcgtc ggcggaattc gggagatcgt catgccgacg
     1741 atcgatatcg acggtgtggg caggatcgcc tttccgatcc tgcccgtcca ggccgaacgg
     1801 ctcgccgcaa ttgccgaggc agccccctat gggcggggcg aaaagacagt ggtcgatcgc
     1861 gaggtccgga gaacctggca ggtcgattcc gccaaggttc ggatcggagg gcgtcactgg
     1921 gacaagacct tggccagtct cgtcgccgac atagcccttg ggctcggtgt gagcgagccg
     1981 gttgcggccg agttctataa gcttcttgtc tacgataccg gcagcttctt cgtcgaccac
     2041 cgcgataccg agaaggtccc cggcatgttc gcgacgatgg tgctcgtgct gccttcgacc
     2101 catagcggcg gcgaattggt cataaagcat ctcggccggg aagtggtgct cgacgcgcgt
     2161 ccggaggaac cgtcggagat cggcttcgcg gccttctacg ccgattgcgt gcatgaagtg
     2221 cgtcctgtta agaccggatg tcgcctgacc ctcgtctata atttgtgttt cgtcggcgag
     2281 aggcgcccgc ttaaggcgcc cgattatcgc gcagagcacg ggcgggtggt ggagttgctg
     2341 cggagctggg cgagtgccga ggacgagcct gacaagctga tcctgccctt ggagcatgtc
     2401 tatacgccgg cggagctgtc gttcagtacc ctcaagggtg ccgatgcggg cgtggcgtct
     2461 gtgctggtca aggcagccgc agaggcggat tgcgatctcc acctcgccct cgtgtcgatc
     2521 gaggagagcg gcagcgccga acataccggc tattacggcc gtcgccgttg gagtcgcgac
     2581 gaggaggaag acgaagaatt cgaggttgcc gaggtcatcg accgggcgct gatcctgtcc
     2641 gagtggcgac atccggatgg cggcgaggcc gggttcgacg tttttccatt cgccgaggac
     2701 gagctgtgcc cgcccgacgc atttgaggat ctgacgccgg acgaggagca ttttcacgag
     2761 gcgacgggca acgaaggcgc gtccttcgag cgcacctatc gtcgtgccgg cttcgtgctg
     2821 tggccggccg cgcggaggct cgcggtgctg aatcaggccg gcctccgcac gacgctgccg
     2881 tatctcgaag atctgacggc gcgctgggag gcgggcaacg catcgatccg gtcgccgttt
     2941 tggcgtgagg ccgatgagct ttcggggcac atgctgcgct cctggtcccg ctcgccttgg
     3001 cgggcggacg acgacgagga agccggccgg atgctcgatt tgcaggtccg gcttggaaac
     3061 gtcgagcgca tcgatgcgtt tctcgccgag ttgtccgcgg aaggccatta cgccgcccac
     3121 gacaacgggg cgatcgtgtg cgctgccgcc cttcttccgg ccacgcgtgc agcggagctg
     3181 ctggtccgga ttgtccgccg taacgctcca gcccgtctcg gcgcctgcgg cgatctgctg
     3241 ctgcgctgcg tcgcggcgcc gatgggtacc accggcgacc tcgggcagat cagcgtagcc
     3301 ttgatcgatg ccttgccggg cgacccgacg aggcgagagg aggtcggcac ctggactcgg
     3361 ccaacgccgg tgacgccggg cttcgtcgtc gatcttttga cggcgacgag ccggatcgat
     3421 gcggggctcg cggcgcgtgc gatcgagcac ctgctggcct ggccgaagac atatagtcct
     3481 gacgacgtgc tggtccccgc cgcccgcatg tttgccaagc tggcggagag caccgcctgg
     3541 ccggcggtgg ggcagctccg ggaggcgtct ctcgaccacc tgcgcaggcg catcgccctg
     3601 ccgctggagg cgccacgcga ctgggccaga gccaatccgc tcgaatgcac atgcggcgac
     3661 tgccgtgcgc tgggcgcgtt cctggttgtg ccagatcaac agcaatggcg cctgaaggcg
     3721 gtccaggatc ggaggaccca cgtcgagcaa agcctcgaac gcgtggcctg cgacctcaac
     3781 ctcacgaccg aaaggcgcgg cagcccgcac acgctggttg ccaccaagaa ccaggctagc
     3841 tacgaacggc gcgcagagca gcgccggcag gatttggagc atgtgtcggc actgggcgaa
     3901 tgagtttggc ggcgccctat ccatgaaggc gcatagagcg accgcgacat cggaaactgc
     3961 cccctatggg cgcagtgcca aatcgcaggt ggtcgcgtat tctcgtgttc tggcgtgtgt
     4021 ttggctgcgg cgtcatagtg ccacggcgaa acaatattgc ccatcttcat cggccgtggc
     4081 gcgccacttc ggaatacgcc gtcgccgcca aatcccgccg atttcccggt gcaggccggc
     4141 aggctgcctc cggaaaccgt gcgttcggct gcttacccga acaccggatc caaaaacgat
     4201 cccgcttgaa tttcgtcata tttccggtta tatgaccgga atcaaatctc gacgacttga
     4261 gaaggaccta tggctagcaa gtcccagcgc aaggcgaccc gtacgcaccg tcgtcgcgcg
     4321 gcggcgcgag gttttgtgcg cgttgaggtt caggcgccca agaaggatgc cggcctcatc
     4381 cgggcgctgg ccgagacgct gcgcggcggg gccgagaagg ccgaggcgct tcgatccacc
     4441 ctcgccaaag ccgtgatgaa tccagagatg gggactgctt tcgacatctt tggctcggaa
     4501 cttcccgacg aagtcttcac aggcgttttt gaccagccgc gccaacgagg ctggcgccgg
     4561 gttgatctgt gaaattcctg ctggacacga atgttatttc cgaaatccgc aagcgcggcc
     4621 gcgcccatcc aaacgttgtc cattgggtca ctcgcacacc ggtcgaggac attgggacga
     4681 gcgtgctcgt gctcgccgaa atccgccatg gtatcgagct gaaacggcgg agcgatccag
     4741 agcaggccga atcgctcgat cgatggtttt tgcaaatgcg gaccagactc ggggggcgcg
     4801 tcctgccgat cgacgagccg gtcgcggaag cctgggcgct cctcggaatt cccaatccgt
     4861 tgcctttgat cgacggcctg ctggcggcga ctgccaaagt gcacggcctg acatttgtga
     4921 cccgcaacat cgcggacatt gtcgtgaccg gcgtgccgct gcttgatccg ttctctggag
     4981 tttcgtagcg cctatccggc ccgtccggtt gcatccgcag gccgcctcac gtttccggcg
     5041 aattgcgacg acgattaagt gcactgaaga tcatctagcc ggccttcgcg accgcgaggg
     5101 tggtggagct aaagtgagtt cgcaatcatc gaatcacttt gaccacgagg aggcgaagat
     5161 gatgt
//
LOCUS       DASWGM010000608         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1428864,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000608 DASWGM010000000
VERSION     DASWGM010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428864"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VGA15_17195"
     CDS             <1..1009
                     /locus_tag="VGA15_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9589470.1"
                     /translation="NTYAGKIEIIPTVNFILKWVRGEVGALKLDENLSEISLITKDAF
                     EPTLPLSVVMHIDYKKAPMIIQRFGDVKKLVEQTLDPMVSAFFKNIAQKMTLIELLQN
                     RAAIQEESGNEMKARFEAYSLELQEVLIGTPRAAAGDQTIENILIQLRSRQVAREQVT
                     TYQEQEKAAVQERTLNEARATAAAQIALTQSLIQVKVHENEGAAALARALKDAETVKV
                     TAAAVGERSRLEGQGEADRALAIGTANAQATKLSVDAYGGPEYRLAEQNFSRFADALT
                     KINQPLVPQFLMSGAQGQENNGGLIPTAMLSSMFGRMMPDALEKLKEEAPKPKPRGNG
                     P"
     gene            complement(1102..2256)
                     /locus_tag="VGA15_17200"
     CDS             complement(1102..2256)
                     /locus_tag="VGA15_17200"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9589471.1"
                     /translation="MDGDARGLWHLYFIGFVGRPIGAAIFGHYGDRIGRKATLIATLL
                     CMGIATFLIAFVPTYASIGIWGAVILTVLRMLQGIGVGGEWGGSVLLAMEWSRTHGQR
                     GLVASWPQFGVPCGLFLANPAVLAFSQLSGDQFSTWGWRIPFALSIILVGVGLWIRLG
                     ILETPVFQQLLDTRKIEKTPIIEVFKKHPKEIFLSALLRMSEQAPFYIFTAFIFAYAV
                     GTLHMSRDLVLIAVLVASCVSFVTIPLSGHISDRIGRRKMYLIGAATVGVFGFLYFGM
                     VDTAIPSLVFIAIVLSLIPHDMQYGPQAALIAEAFTPRLRYSGASLGYQLASVIAGGP
                     APLIATALFAAYHSGYAISVYIAACAVVSLVAAWFMPDYTGRDISMEYDD"
     gene            complement(2139..2429)
                     /locus_tag="VGA15_17205"
     CDS             complement(2139..2429)
                     /locus_tag="VGA15_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589472.1"
                     /translation="MAMAITHATLSASDHSMQLRKAVIASTIGTAIEWYDFFLYGTAA
                     GLIFGKLYFPNEDALTATLAAFGTFISSALSGDRSVPRFLVTMVIASAAKRH"
     gene            complement(2750..>3364)
                     /locus_tag="VGA15_17210"
     CDS             complement(2750..>3364)
                     /locus_tag="VGA15_17210"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589473.1"
                     /translation="YFANANAGAAVLNTNTCTAVALLVWTLMDKMAYGKPSVLGAVNG
                     MIAGLVAITPGAGYVDGVGAIIIGIVAGIIPWLAMNKLQKASFMMKVDDTLSVLSTHG
                     VAGLTGGLLTGILANPAMLLYIGTDKEAPGVNVTGLLYGNAPQLLLQAEGAAFIIVYN
                     VIMTFIILKVISFIVPLRMDEATLKVGDDAVHGETAYAIGVEGE"
BASE COUNT          758 a          992 c         1042 g          572 t
ORIGIN      
        1 caatacctac gccggcaaga tcgagatcat ccccacggtc aacttcatcc tcaaatgggt
       61 ccgcggtgaa gtcggcgcgc tgaaactcga cgaaaatctt tccgagatat cgctcatcac
      121 caaggacgcg ttcgagccga ccctgccgct ctcggttgtc atgcacatcg actataaaaa
      181 agcgccgatg atcattcagc gcttcggcga cgtgaagaag ctggtcgaac agaccctcga
      241 cccgatggtc agcgcctttt tcaagaatat cgcgcagaag atgacgctga tcgaactgtt
      301 gcagaaccgg gccgcgatcc aggaggagtc ggggaacgaa atgaaagcga ggttcgaggc
      361 ctactcgctc gaattgcagg aggtgctgat cggcacgccg cgggccgcgg ctggcgacca
      421 gaccatcgag aatatcctca ttcaactgcg ttcacgccag gttgcgcgcg aacaggtgac
      481 gacctatcag gagcaagaga aggcggcggt gcaggagcgg accttgaacg aggccagggc
      541 gaccgcggcg gcgcagatcg cgctgacgca gtcgctgatt caggtcaagg tgcatgagaa
      601 cgaaggtgcc gcggcgctgg cgcgggcgct gaaggatgcc gagaccgtca aggtgaccgc
      661 ggccgcggtc ggcgagagat cgcggctcga ggggcagggc gaggcggacc gggcgctggc
      721 gatcggtaca gccaatgccc aggccaccaa gctctcggtc gatgcctatg gcggccccga
      781 atatcggctt gccgaacaga acttctcgcg tttcgccgac gccttgacca agatcaatca
      841 accgctggtg ccgcaatttc tgatgtccgg cgcgcagggc caggagaaca atggcggcct
      901 catcccgacg gcgatgctga gctcgatgtt cggacgcatg atgcccgatg ccctggagaa
      961 gctgaaggaa gaggcgccaa agcccaagcc tcgcggcaac ggcccatagc ggcagcatca
     1021 agcccatccc ggacacgccc gacaaaatcg agggcctgga caagcccagg ccctcttgaa
     1081 caatcgacgt tacaccttgt gtcagtcgtc atactccatc gaaatgtccc tgccggtgta
     1141 gtcaggcatg aaccacgccg ccacaaggct tacgaccgcg caagcggcga tgtagacgga
     1201 aatcgcgtag ccggagtgat aagcggcgaa tagggctgtc gcgataaggg gcgccggacc
     1261 gccggcaatc accgatgcga gttgatagcc cagtgaggca ccgctgtagc gcaatcgcgg
     1321 cgtaaaggcc tctgcgatca aggctgcctg cggcccatat tgcatgtcat gcgggatcag
     1381 tgacagcacg attgcgatga acaccaggga tggaatcgcg gtgtccacca ttccaaaata
     1441 caggaaaccg aacacgccca ccgtcgccgc gccgatcaaa tacatcttcc tgcgcccgat
     1501 gcggtcggag atgtggcccg acagcgggat ggtgacgaac gagacgcaag aggcaaccag
     1561 cacggcgatc aagaccagat cgcgcgacat gtgcaacgta ccgacggcgt aagcgaagat
     1621 gaacgcggtg aagatataga acggcgcttg ctcggacatc cgcagcaatg ccgacaggaa
     1681 tatctccttc ggatgcttct tgaacacctc gatgatgggg gtcttctcga ttttcctggt
     1741 atccaggagc tgttggaaca ccggcgtctc gaggatgcca aggcgaatcc atagtccgac
     1801 accaaccagg atgatcgaga gcgcgaaagg aattcgccag ccccaggttg agaactggtc
     1861 acctgacaat tggctgaatg cgaggactgc agggtttgcc agaaacaggc cgcacggcac
     1921 gccgaattgc ggccaggatg ccaccaaacc gcgttgaccg tgggtgcgcg accattccat
     1981 cgccagcagc accgatccac cccattcgcc accaacgccg ataccctgca gcatccgcaa
     2041 caccgtaagg atgaccgcgc cccagatgcc gatcgacgca taggtaggca cgaatgcgat
     2101 gaggaaggtc gcgataccca tgcacagcag cgtggcgatc agtgtcgctt tgcggccgat
     2161 gcgatcacca tagtgaccaa aaatcgcggc accgatcggt cgcccgacaa agccgatgaa
     2221 ataaaggtgc caaaggccgc gagcgtcgcc gtcaatgcat cttcgttggg aaagtagagc
     2281 ttgccgaaga taagcccggc agccgtgcca taaaggaaga agtcgtacca ctcgatggcg
     2341 gtgccgatgg ttgatgcgat gaccgccttg cgcaactgca tgctgtgatc agatgcggac
     2401 aacgtcgcat gtgtgattgc catagccatt gcatggccct ccctggttgg ttgtctcctt
     2461 gtcccgtggg aaccgggttc tcaacggttc atttaagggg aatctataat cggctcgcgg
     2521 cgaccgaaag gtgcatctca ccgaaggcgg tcatattgct gcatcgcagg agattcgcgc
     2581 gcgaccttgg tgagcaacca gtgttcggtg cacgtcggtc tcatccgcga atcggaatcg
     2641 cggcggagcc tgtgacaggt cgacatcgat gtgccatgcg cgtaaaaatc cgcggggacc
     2701 ggagtcgacc cgcggattcc atgtcgtgtg caaaggtgga gagggtcggc tattcgccct
     2761 cgacaccaat cgcataggcc gtctcgccgt ggacggcgtc gtcacccacc ttcagggttg
     2821 cctcatccat gcgcagcggc acgatgaacg agatcacctt gaggatgatg aaggtcatga
     2881 tcacgttgta gacgatgatg aacgcggctc cctccgcctg cagcagcagt tggggggcgt
     2941 tgccgtagag caagccggtc acgttgacac cgggcgcttc cttatcggtg ccgatgtaca
     3001 gcagcatcgc ggggttggcc aggatgccgg tcagcaagcc gccggtgagg ccggccacgc
     3061 cgtgggtcga gaggacactc agcgtatcgt ccaccttcat catgaagctg gccttttgca
     3121 gcttgttcat cgccagccag ggaatgatgc cggcgacaat gccgatgatg atcgcgccca
     3181 cgccatcaac gtacccggcg ccgggcgtga tggcgacgag gcccgcgatc atgccgttga
     3241 ccgcgccgag caccgacggc ttgccatagg ccatcttgtc catcagcgtc cagaccagaa
     3301 gggccaccgc ggtgcaggtg ttggtgttga gcacggccgc gccggcattg gcgttggcga
     3361 aata
//
LOCUS       DASWGM010000609         5643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2011588,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000609 DASWGM010000000
VERSION     DASWGM010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011588"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /gene="groEL"
                     /locus_tag="VGA15_17215"
     CDS             <1..193
                     /gene="groEL"
                     /locus_tag="VGA15_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964085.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is not
                     essential for growth; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX9589474.1"
                     /translation="RNGASVDLVKAGIIDPVKVCYSAVRNAASVAGLILTTQTLIAKK
                     PDDYDPTAGPALGGGAELL"
     gene            complement(232..2172)
                     /locus_tag="VGA15_17220"
     CDS             complement(232..2172)
                     /locus_tag="VGA15_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX9589475.1"
                     /translation="MDQRDVRAAWEKFVERGALSADLRSSVAASWQRSKNHRVTIDRA
                     RAPLVADAELFRHRSKHASLRHAARCALENSKTFLSDANSIMILTDPTGLIIDTQGDD
                     RVIDAGRTVHLEHGGRWSEADIGTNAIGAAIAESKPVQIRGAEHFCSEVQRWTCAAVP
                     VHDPTDGELLGIVDISGPASIFNPQSLALAVSVGHHVESVLAQSVRRDHEELLCHFLA
                     KRSLWVNEEFIVLDRRGMILHATERALNALQNNRHGIDDDAPTRFLKTLPFDEWPIKL
                     KELLPNASFDLVENETSGIGAIMVLHARRRLPIADRDVQKPKPAFERRETASSSHPRK
                     DLTPGPQHLEDKVGSATSRFIARDPTVRAIVRQVETAAARKMPILIRGETGTGKEQLA
                     RHAHAASGRTGAFVPVNCAALPESLIEAELFGYADGAFTGARRGGAIGLVKEADGGTL
                     FLDEIGDMPVSLQAVLLRLLDDWTVRPVGGVRAKVDVFLVSATNASLDKAIAEGRFRS
                     DLLYRLNTLEVTLPRLRDRTDFDAIVHHLLGTIDPNCKITSATIAHLAARPWPGNIRE
                     LRNMLARFTLATADGFVDEVGLEAMICQAPPTTPGSLHDIQRARILVVYAETAGNISE
                     TARRLGVSRNTIYRALGQKKPR"
     gene            3319..4353
                     /locus_tag="VGA15_17225"
     CDS             3319..4353
                     /locus_tag="VGA15_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9589476.1"
                     /translation="MVSQSALPRRLLGRTAIQVSALGFGAAPLGDLYRQLDDGRAIAA
                     VERALALGINLIDTSPLYGHGLSEHRCGTAIRRLPRDQIVVCTKVGRWMDPFHGRGNG
                     SGFSGGQPHRAVVDYSYDGTMRSVEQSMLRLGTDRLDLLLIHDVDVWTHGADAIEQRF
                     AEAMAGAYVALDRLRSEGVVAGIGIGVNEAEMCVRFAQAGSFDTILLAGRYSLLEQPA
                     LEHFLPLAQRRGIGVMLGGVFNSGILATGAVKGAKYNYQDAPPEILAKVARIERVCTA
                     HGVALPTAALHFALGHPAVASVVLGAETPQEVERNVAGLSSAVPAALWSDLKAERLLD
                     PDAPVPAVAG"
     gene            4353..5198
                     /locus_tag="VGA15_17230"
     CDS             4353..5198
                     /locus_tag="VGA15_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9589477.1"
                     /translation="MRIDAHHHLWSLAQGDYGWLTSELAPIYRDFGLPDLAPHLAAAG
                     IEGTILVQAAPTEAETLYLLDIAETAELVRGVVGWTDFDAADGAARIDALAARKLLVG
                     LRPMVQDIDNDDWLLGPTLAPLLTAMVRNGLVFDALVLPRHLPRLLRVIDSHPDLEFV
                     LDHCGKPRLATGEIAIWQRDIALLAEHPNIVCKLSGLVTEAVPDWQISDLRQAVDHVR
                     GCFGPQRMLWGSDWPVVNLAGGYAKWLAAAETLLADLSPDEQADIFGGNAARIYLSSR
                     GRRTC"
     gene            5293..5616
                     /locus_tag="VGA15_17235"
     CDS             5293..5616
                     /locus_tag="VGA15_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589478.1"
                     /translation="MPIVTDAAFILPDDFADAALMGRIWRPDVASPSVVAIRQDGVFD
                     ITENFPTGSQLAEVADPGAALRAARGERVGVLQDLLTNTPPDNRGIRHSVRRSPTCRR
                     LRSNH"
BASE COUNT         1055 a         1661 c         1799 g         1128 t
ORIGIN      
        1 gcgcaacggc gcatccgtcg atcttgtcaa ggccggcatt atcgatccgg tcaaggtctg
       61 ctacagcgcg gttcgcaatg cggcctcggt cgccggtctg atcctgacga cgcagacgtt
      121 gatcgccaaa aagccggacg attacgatcc aacggctggg ccagccctgg gcggcggcgc
      181 cgaactattg tgattttgcc gcaatacatt ggaggtgctc gtacccggag gtcatctcgg
      241 ctttttttgt ccaagtgccc gatagatagt atttctcgag acaccgagac gccgcgcagt
      301 ttcgctgatg tttccagcgg tttcggcata gaccacaagg attcgggccc gctggatgtc
      361 gtggagtgat cccggcgtcg ttggcggggc ctgacagatc atggcttcca acccgacctc
      421 gtcgacgaag ccgtcggcag tggcgagggt gaagcgtgcc agcatattgc gcagttcacg
      481 gatgttgccg ggccagggac gcgcggcgag atgagcaatg gtcgccgacg tgattttgca
      541 atttggatcg atcgtcccaa gcagatggtg aacgatggcg tcgaagtcgg tacgatcgcg
      601 gagtcgcggc agcgtcacct ccagggtatt gaggcgatag agcaggtcgg agcggaagcg
      661 cccttcggca atggccttgt cgaggctggc gttcgtagcg gagacgagaa agacatcgac
      721 cttggctctg acgccgccga cggggcgcac ggtccagtcg tcaagcaggc gtagaagcac
      781 ggcttgcagc gagacgggca tgtcgccgat ctcgtcaagg aagagcgtgc cgccgtccgc
      841 ctccttgact agtccaattg cgccaccgcg ccgcgcgccg gtgaatgcgc cgtcggcata
      901 gccgaatagc tcggcctcga tcaggctttc gggaagggcg gcgcaattga cggggacgaa
      961 ggcgccggtc cgcccgctgg cggcatgggc gtggcgggcg agttgttcct tgccggtacc
     1021 ggtctcaccg cgaatgagga ttggcatctt gcgtgctgcc gcagtctcga cctgacggac
     1081 aatggctcgc acggtcgggt cgcgggcgat gaagcgggat gtcgcgctcc cgaccttgtc
     1141 ctcaagatgt tgaggtcccg gcgtcagatc tttgcgaggg tgcgacgaac tcgcggtctc
     1201 gcggcgttca aatgcgggtt tcggtttctg aacgtcgcga tcggcgattg gcaggcgccg
     1261 tcgggcatgc agcaccatga tagcgccgat gccggaggtt tcgttctcga cgagatcgaa
     1321 gctcgcgttc ggaagcagtt ctttcaattt gatcggccac tcatcgaagg gaagcgtctt
     1381 taaaaagcgg gttggcgcat cgtcgtcaat gccgtgacga ttgttctgga gagcattcag
     1441 agcgcgctcg gtggcgtgga ggatcatgcc tcgacggtcg agcacgataa actcctcgtt
     1501 cacccacaac gacctcttgg cgagaaagtg gcacagcagc tcttcgtgat cacgtcggac
     1561 cgattgcgcc aatacgcttt ccacatgatg accgacagag acggccaaag ccaggctctg
     1621 cggattgaag atgctggccg ggcccgagat gtccactatg ccaagcagtt cgccatcggt
     1681 aggatcgtga accggaacag cagcacaggt ccatcgctgc acctccgagc aaaaatgctc
     1741 cgccccgcgg atttggacag gtttcgattc cgctatcgca gcgccgatcg cgttggtgcc
     1801 aatgtcggcc tcgctccaac gtcccccgtg ttccagatga accgtgcgac cggcatcaat
     1861 gaccctgtca tcgccttggg tatcgatgat caatccggtg ggatcggtga ggatcatgat
     1921 tgaattcgcg tcgctcagga atgttttcga attctcgagc gcgcagcgcg ctgcatgacg
     1981 cagtgaagcg tgcttggagc ggtgacgaaa cagttcagcg tctgcgacaa gcggcgcccg
     2041 cgcgcggtcg atcgtgaccc gatgattttt ggatcgctgc cacgatgcag cgaccgacga
     2101 tcttaaatcg gccgataacg ctccgcgctc gacgaacttc tcccacgcgg cccgcacgtc
     2161 tcgttggtcc atcccggctc ctccgacgtg acaaagtccg aaacccgcac actagcgggc
     2221 atcgtgatca atcacctgaa tgcggggtgc tcgtcgcaat tgccgttgcc atcacgcaaa
     2281 gcgcaatttc aaaccctttc ccgccaccga aacggtgggc tcctggatcg agtcgaagac
     2341 ctgtcaggcg ctcttgtcat gatgactacc gcgtcatcca accagacatc ccttccagcg
     2401 ttcacttccc cgacgtctca tgcggttctt tagcgccgct gatcagcaca gcctatactt
     2461 cttggctact tggcgcaaag gaggagtcca aagatccgga gtctttgccg agctcatcgg
     2521 cgatgcgtgt cgcggaacag gcccaagcat ccggtttgaa ctccaaaatg ttcgcctcgc
     2581 aggaattcgc aacttgcgga gggctcgctg cagctcagcc ggccggatct gataggtgca
     2641 gcgcaacatc cagggatcgg gtggctaacc gttatcccga ctgcctttca attcaatgac
     2701 gaaacagccc gctactatac cgctgcttgt aacgacctac ggcccgcttg caccaacggt
     2761 gatgttcatg agaaaattgc gtgcgacatc tgcgattcaa cgccatcgtt gataggctct
     2821 cgttagaact tggttgctcg agctagatca aacttgccca tgatggattg gaacgagaga
     2881 tcaagagttt caattagtta gaacgccgcg tgtgcacgat gtgtgcaccg agagatcaac
     2941 ggaaatccaa caatagccaa cgttgagtgg ccgcgctctg catctcggtg tttggtcgtt
     3001 tgatccagaa gtcgaggagc ttcggcggcg tttctgcggg gacgcacgga gggaactttc
     3061 gtcgagcacg aagcgatcta tcgggcgatc gcggcgcgcc agccggatca agcacgaaac
     3121 gaaatggcca accatctcga ttgcgtgccg cgagagttgc gctcctttgt catcagggca
     3181 tccgggtctc cttgaggaat cgaacctcgc atgccgcacg ctcacgcgcg ttgacaggta
     3241 gctttaaaga aatacgctgc ccgtaaatct caatgatggc ccagcgcggc tggagcacga
     3301 cggggaaaaa cgtccaccat ggtttcgcag tcggcactac cgcgacgtct gctggggcgg
     3361 accgcgattc aggtttcggc gctgggcttc ggcgccgcgc cgctcggtga tctctatcgg
     3421 cagctggatg acggcagggc catcgctgca gtagagcgcg cgttggcgct gggcatcaat
     3481 ctgatcgaca cctctccgct ttatgggcac ggcctgtccg agcatcgctg cggcacagcg
     3541 atcaggcggc tgccgcgcga ccagattgtg gtctgcacca aggtcggccg ctggatggac
     3601 ccgtttcacg ggcgaggcaa cggctctggc ttttccggtg gtcagccaca ccgggcagtg
     3661 gtcgactatt cctatgacgg caccatgcgt tcggtggagc agtcgatgtt gcgccttggc
     3721 accgaccggc tggacctgct gctgatccat gacgtcgatg tctggaccca cggcgccgat
     3781 gcaattgagc agcggtttgc cgaagcgatg gccggcgcct atgtggcgct cgaccggctt
     3841 cgcagcgagg gcgtggtcgc aggcatcggg atcggcgtga acgaggccga gatgtgcgtg
     3901 cgtttcgcgc aggcgggatc gttcgatacc atcctgctgg ccggccgcta ttcactgctc
     3961 gagcagcccg cgctcgagca tttcctgccg ctcgcgcaac gacggggcat tggtgtgatg
     4021 ctcggtggcg tattcaattc cggtattttg gcgaccgggg cggtgaaggg cgccaaatac
     4081 aattatcagg acgcgccgcc ggagattttg gccaaggtgg cacggatcga acgtgtctgt
     4141 acggcccatg gtgtcgcgtt gcccacggcg gcgctgcatt tcgccttggg ccatccggct
     4201 gtcgccagcg tggtgctggg agcggaaacg ccgcaggagg tcgagcgcaa cgtggccgga
     4261 ttgtcctctg cggtgccggc ggcgctttgg agcgatttga aggccgaacg cctgctggat
     4321 ccggacgcgc cggtaccggc ggtggctggc tgatgcggat cgacgcgcat catcatctgt
     4381 ggagcctcgc gcagggtgac tatggctggc tgacgtcgga gctggcaccg atctatcgcg
     4441 atttcgggct gccggacttg gcgccgcatc tcgccgcagc cgggatcgag ggaacgatcc
     4501 tggtgcaggc cgcgccgacc gaggcagaga cgctgtatct gttggatatt gcagagaccg
     4561 ctgagctggt gcgcggcgtg gtcggctgga cggatttcga cgctgcggac ggcgccgcgc
     4621 gcatcgatgc gctggcagcg cgaaaacttc tggtggggtt gcggccgatg gtacaggaca
     4681 tcgacaatga tgattggctg cttggtccga cattggcgcc cctgctgacg gcgatggtcc
     4741 gaaacggttt ggtgttcgac gcgctggtgc tgccgcgcca tttaccgaga ctcctgcgag
     4801 taatcgacag ccatccagat ctggaattcg tgctggacca ttgcggcaag ccgcggctcg
     4861 caaccggcga gattgcgatt tggcagcgcg atatcgcgtt gcttgctgaa caccccaaca
     4921 tcgtctgcaa actatcgggc ctagtgacag aggccgtgcc ggactggcag atttcggatc
     4981 tccgccaagc ggtggatcat gtccgcgggt gcttcgggcc acaacgtatg ctgtggggga
     5041 gcgactggcc ggttgtcaat ctggcgggcg gttatgcaaa atggttagct gccgccgaaa
     5101 ccttgctggc tgatctgtca cccgacgaac aggcagatat cttcggtggc aacgcggcgc
     5161 gcatctatct ctcaagccga ggaagacgga catgttgaag acgattgaca tggcgtgttg
     5221 cggtcatcga gcgagtcgct tctacgcacg ctgccctgct cgcacagacg ctactccgcg
     5281 aggatcacca cgatgccgat cgtgacagac gccgcattca ttctgccgga cgatttcgcc
     5341 gacgccgccc tgatggggcg gatctggcgc cccgatgtcg ccagcccctc ggtagtggcg
     5401 atccgccagg acggcgtgtt cgacatcacc gagaatttcc ccaccggaag ccagcttgcc
     5461 gaggttgccg atcccggcgc cgccctgcgc gccgcgcgtg gcgaacgggt gggcgtgctg
     5521 caggacctgc tgaccaacac accgccagac aaccgcggaa ttcgccattc cgtgcgccga
     5581 tcgcctactt gccggcgcct gcggtcaaac cactgacgat gtagcgctca aggaaaattc
     5641 cga
//
LOCUS       DASWGM010000610        15501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2150346,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000610 DASWGM010000000
VERSION     DASWGM010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15501
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2150346"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VGA15_17240"
     CDS             complement(<1..579)
                     /locus_tag="VGA15_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9589479.1"
                     /translation="MRSASGGPRVLLRRLRETMAEQVSAQERLDKIVVLIAANMVAEV
                     CSTYVLRVDNTLELYATEGLNRDAVHRTVLSAHEGLVGLVASEATPLNLSDAQTHPAF
                     SFRPETGEEIYHSFLGVPILRAGNTLGVLVVQNRAKRTYVEEEVEALQTTAMVLAEMI
                     ASGELSALAQPGAEPAARHSVHKTGAILSDGIA"
     gene            complement(846..2102)
                     /locus_tag="VGA15_17245"
     CDS             complement(846..2102)
                     /locus_tag="VGA15_17245"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX9589480.1"
                     /translation="MGRLVMKFGGTSVANIDRIRNVARHVKREVDAGHDIAVVVSAMA
                     GKTNELVEWCRDASPMHDAREYDAVVASGEQVTAGLLAIALQAIGIQARSWQGWQIPI
                     RTSDAHASARILEIDGSELINRFKDRKEVAVIAGFQGINPQTGRITTLGRGGSDTSAV
                     AIAAAIHADRCDIYTDVDGVYTTDPRVVPKARRLDRIAFEDMLELASQGAKVLQVRSV
                     ELGMVHNMPIFVRSSFDKPDDINPHANQPPGTLICSEEEIMESHVVTGIAFSKDEAQI
                     SVRQIQDKPGVAAAIFGPLAEANINVDMIVQNVSEDGATTDLTFTVPASDYTRARDTI
                     TSAKSRIGYARLDSATDVAKVSVIGSGMRSHAGVAAQAFAALAARNINIRAITTSEIK
                     FSVLIDAAYTELAVRTLHTLYGLDHT"
     gene            2274..2741
                     /locus_tag="VGA15_17250"
     CDS             2274..2741
                     /locus_tag="VGA15_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX9589481.1"
                     /translation="MGRYIDDILQPGEKVLYSTNAHWIFYWPAIVAWIVAVVLFFLSR
                     ATVNESIVLLCLSASAVVAIAALYWTFKAWFHRWTTETDVTNLRVVHKTGFIKRRTFE
                     MSLDKVESVDVNQSILGRLLNYGNVTVRGVGEGAETITTIASPLDFRNHITAR"
     gene            2758..3528
                     /gene="ubiG"
                     /locus_tag="VGA15_17255"
     CDS             2758..3528
                     /gene="ubiG"
                     /locus_tag="VGA15_17255"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX9589482.1"
                     /translation="MAMQQNSPAGLSGAPASTVDPAEIAKFSKLSDEWWDPNGKMAPL
                     HKINPLRLTYIRDAACRKFERNVKSLNCLSGLRILDIGCGAGLLCEPFTRLGAQVIGV
                     DPSATNIAAAKLHADKAQLSIDYRCTTVEQMDVRERFDIVLAMEVIEHVTDVGVFLNR
                     CAAMLKPGGLMVISTLNRNWKSFALAIVGAEYVLRWLPRGTHQWDKFVTPDELAHHLE
                     NNKLAITEQAGVVYSPFADRWSISSDMDVNYMVVAEGV"
     gene            3692..4591
                     /locus_tag="VGA15_17260"
     CDS             3692..4591
                     /locus_tag="VGA15_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9589483.1"
                     /translation="MLSIATLWIPFTIVAALGQVARNAMQRQLTAPLGTWGATNIRFL
                     FGFPFSLIFFAVVIAATGDAVPWPTPAFWPWLLLGALSQIVGTGMMLLAMNDRSFLVT
                     TAYLKTEAIQTAIFGFVFLGDHLTLLKVIAILIATAGVVIAALRPGAAKGFADLKPTL
                     LGLAAAAAFALSAVGFRGAVIVVPGVSFVTAASYTLVFGLFVQTLALSIYLLVRAPDV
                     LAKILGLWKPSLFAGFMGAFASQFWFLAFALTAAANVRTLALVEVLFAQAVAYYSFKQ
                     PLSGRELGGIALIVIGVALLVSV"
     gene            complement(4748..5224)
                     /locus_tag="VGA15_17265"
     CDS             complement(4748..5224)
                     /locus_tag="VGA15_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX9589484.1"
                     /translation="MIRYSLRCERGHAFESWFQSSSAYETQEKRKLVNCPVCGSAKVE
                     RAIMAPQIVSKKGRDSAVPAPAAPTPTDVTTPTSTPLLMAQERELRAKLRELRDHIVK
                     NADNVGERFPNEARKMHYGDIEHRPIYGEASPDEARALIEEGVEVSPLPVLPDDRN"
     gene            complement(5221..6099)
                     /locus_tag="VGA15_17270"
     CDS             complement(5221..6099)
                     /locus_tag="VGA15_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX9589485.1"
                     /translation="MSADLSFTAAMVQMRTGLLPEPSLEQGTKLIRQAAAEGADYVLT
                     PEVSNMMQLNRTALFEHLAAEEDDLSLKAYRALAAELKIHLHVGSLALRASPERAVNR
                     SFLIGPDGLVIASYDKIHMFDIDLPGGESYRESANYQPGETAVISDLPWGRIGLTICY
                     DVRFPALYRALAESGAAFLTVPSAFTRKTGEAHWHTLLRARAIENGCFVFAAAQGGMH
                     ENKRETYGHSLIVAPWGEVLAEGGVEPGVFMAKIDPAKVETARKTVPSLQHGRRFSVA
                     DPKAGPEHLHLVRGSA"
     gene            complement(6096..6371)
                     /gene="grxC"
                     /locus_tag="VGA15_17275"
     CDS             complement(6096..6371)
                     /gene="grxC"
                     /locus_tag="VGA15_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX9589486.1"
                     /translation="MTAAIEIFTRPGCGYCSAAKSLLTRKKAAFTEHDVAVDSTLRQR
                     MWDRAGAGSTFPQIFIGETHVGGCDELYALDRAGKLDPMLAGEKANS"
     gene            complement(6401..7210)
                     /locus_tag="VGA15_17280"
     CDS             complement(6401..7210)
                     /locus_tag="VGA15_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX9589487.1"
                     /translation="MDAETSSSRSISTHLRGALNTCRGAWAHAARLALDIALPTLCVA
                     CREPVAGEGVCATCWAKLSFIAQPYCPRLGIPFVYDPGPGLLSMEAIADPPAYARARA
                     AVRYDDVARTLVHALKYQDRTDLAPAMGRWMARAGRELLGEADALVPVPLHWRRGWSR
                     RYNQSGALARIIERQSGVKVTTEALRRVRPTQQQIGLSRSQRASNVQGAFKVEASRQS
                     EIQGRRVILIDDVLTSGATVDACARALLRAKAASVDVLVFARVVDTHKAPI"
     gene            complement(8818..9285)
                     /locus_tag="VGA15_17285"
     CDS             complement(8818..9285)
                     /locus_tag="VGA15_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589488.1"
                     /translation="MSARSLYETLPIGKWSPRSLASMGPGKAMRRAKIAAIGLACIAL
                     AACVLLASSNPTKAEGDIDGLLKAYDSKDPLEKQYARIHADLIETGISAANRAIVGKK
                     QTPLYWPPSNLVLTGDQVLDMLRREVQKIPPLGTVATDLGILVVLERTFACNH"
     gene            9374..9559
                     /locus_tag="VGA15_17290"
     CDS             9374..9559
                     /locus_tag="VGA15_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589489.1"
                     /translation="MRFEVGQTFQGTVDISMRAEVIEVSNDGRDAKLKLLTHDGSAFE
                     LNAGAVMAGHQKWRLVP"
     gene            9616..9960
                     /locus_tag="VGA15_17295"
     CDS             9616..9960
                     /locus_tag="VGA15_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589490.1"
                     /translation="MMAAKVTSALIKSVQTNPDPFMTYGDFGRAFGLSQKVTVSWANR
                     GTLDAVASALKIDPKVAIDLTFLLRSKAGGFPMVIDGLPYVPDDKVQEQRARDVADQI
                     IAQFKLTAKNPY"
     gene            10074..10991
                     /locus_tag="VGA15_17300"
     CDS             10074..10991
                     /locus_tag="VGA15_17300"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HEX9589491.1"
                     /translation="MPILVDPQMVADDSGSEPQSPIFVLAGFIAPAAKWADFAVEWEA
                     ALNLSPKLDYFKMTEAANLSGQFSRRKGWDEAKRDDRLVTLTRIIRKYASVRVSAWIM
                     HDDYDTHIKSLKAPIRHLALDSPYVMLLQQLVLAIAVFGDRHGIKDHCDYIFDEQGKL
                     AEEATYWWPQFKWHVGHSSRGDLAKFVGSPPIFRDEKSFLPLQAGDLYAWQIRNQYAR
                     NHAVPNQTIFVPHSRILGMLDTIPLINREYPTDEVLRLREHLINAGEQYAKAFPDVPL
                     IGPHADRKDRRKAHKQARKARAVSNPPTS"
     gene            complement(11157..11888)
                     /locus_tag="VGA15_17305"
     CDS             complement(11157..11888)
                     /locus_tag="VGA15_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589492.1"
                     /translation="MTNDDRHNNIAGVLLILGLGLLGWLWCVLLVATSGEQAAALGAI
                     IGGMIGAGGAVYAVYMTLTRQRREDIAKVCSAVRIEVTTYCKHVIGALNICEQIATKG
                     LAVPMQDAVYVAKSLVEPIIYPAVADRIGLFPHPQATIEFYMRIAEAKAMLLAMQMKT
                     NSPSAVNASAIPLIAPANAEAVAECFITALQLAKHIVAADDPSRTELDLWVQKITLGT
                     IDAALASAKVVFPNATSFQDDQPAA"
     gene            complement(12613..12771)
                     /locus_tag="VGA15_17310"
                     /pseudo
     CDS             complement(12613..12771)
                     /locus_tag="VGA15_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734500.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
     gene            12957..13808
                     /locus_tag="VGA15_17315"
     CDS             12957..13808
                     /locus_tag="VGA15_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9589493.1"
                     /translation="MAPNPSTAPILFDRALLRARQSRALRSGSATFLLDRVAEDLAER
                     LHAVLREFKTAADIGTVGDQVRDTVAGRVDTIARIELPDRESEPIPLAPESLDLAVSA
                     LAFQFVNDLPGVLTQIRRALKPDGLLLAAMIGGDTLTELRQSFAAAEAELEGGVSPRV
                     APFADLRDVGALLQRAGFALPVTDVDRIVVRYDSAFALMQDLRRMSATNILVERRRTP
                     TRRATLLRMAQFYAERFADSDGRIRATFDVIWLSGWAPHDSQQKPLRPGSAKASLAEA
                     VKKVAPK"
     gene            complement(13931..14341)
                     /gene="mutT"
                     /locus_tag="VGA15_17320"
     CDS             complement(13931..14341)
                     /gene="mutT"
                     /locus_tag="VGA15_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEX9589494.1"
                     /translation="MPDFKLTLVVACALIDADKRVLIAQRPEGKTLAGLWEFPGGKLE
                     PGERPEATLIRELHEEIGITVSEACLAPLTFASHAYDDFHLLMPLYICRRWEGQVTAR
                     EGQKLAWVRANKLRDYPMPPADIPLIPHLIDLLM"
     gene            14514..>15501
                     /locus_tag="VGA15_17325"
     CDS             14514..>15501
                     /locus_tag="VGA15_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX9589495.1"
                     /translation="MHTFFRLIGRFRRDKRGNLAVIFAVACVPLISAIGCAVDYTRAV
                     TIRSKLQAAVDAASVGSVARLSPASTAAATMSSDGPIQAGVDDATKIFNGNVSGMTGY
                     TLNSFTPSVTKSGVKIVSDIRFSADVPTMFLGVIGKSKMTVTGDSGAVNSLPPFLDFY
                     LMLDVSGSMGLPSTNAEQTRLSQISPDMFVQYPGGCTLACHFTVSNSCANGNQKYPTN
                     NECLGYWISRVSPTAYANLVSSPTAWNAAGTSLVSKPVPGLPNSMISGLPNSLSSGDP
                     NRLPAVSSCATDGTANCIQLRADAVGYAVNQLFITANDPTYQKVPGQFRIGLY"
BASE COUNT         2928 a         4728 c         4765 g         3080 t
ORIGIN      
        1 cgcgatgccg tcggacagga tcgcgccggt cttgtgcacc gaatgccgcg cggccggctc
       61 ggcgccgggc tgcgccagcg ccgacaattc gcccgaggcg atcatctctg ccagcaccat
      121 cgcggtggtc tgcagcgcct ccacctcttc ctcgacataa gtgcgcttgg cgcggttctg
      181 caccaccagc acgccgagcg tgttgccggc gcgcaggatc ggaacgccga ggaaggaatg
      241 atagatttcc tcgccggtct ccgggcggaa cgagaacgcc ggatgggttt gcgcgtcgct
      301 gagattgagc ggggtcgcct cgctcgccac caggccgacg agaccctcat gtgcgctcag
      361 aacggtgcgg tgaacggcgt cccggttgag gccttcggtg gcatagagtt ccagcgtgtt
      421 atcgacgcgc agcacatagg tcgagcagac ctcggccacc atgttggccg cgatcagcac
      481 cacgatcttg tccaatcgtt cctgggccga gacctgctcc gccatggttt cgcggagccg
      541 tctcagcaag acgcgggggc ctcccgacgc gctccgcatg tgctggtacc ctcccccgcg
      601 aagccagcac ccggggtggc cggcggctgg cggtaagttc aagaaaagca acaattttag
      661 tcattcggcg ccgccgcaaa cttccaaacc ggccgaatca gcgtggctaa aactctcgca
      721 cagtttgcgg cagccacctc atcaggccgt atagcgaatt ggggcttgtt ttgccaagca
      781 aaacgcctgc cagaggccat aacgtctttg tcagcccatg ctgcgcgcgc taaggaaaaa
      841 ttattctaag tgtgatcgag cccatagagc gtatgcagcg tgcggaccgc aagctcggta
      901 taggcggcgt cgatcagcac cgaaaacttg atttcggacg tggtgatggc gcggatattg
      961 atattgcgcg cggcaagggc cgcgaacgcc tgcgcggcga cgccggcatg gctgcgcatg
     1021 ccgctgccga tcaccgagac tttcgcgaca tcggtggcgc tgtcgaggcg ggcatagccg
     1081 atgcggctct tggccgacgt gatggtgtca cgggcgcggg tataatccga ggccggaacg
     1141 gtaaaggtga ggtcggtggt ggcgccgtcc tccgagacgt tctgcacgat catgtcgaca
     1201 ttgatgttgg cctcggcgag cgggccgaag atcgcggcgg cgacgcccgg cttgtcctgg
     1261 atctggcgga ccgaaatctg ggcttcgtcc ttggagaagg cgatgccggt gacgacgtgg
     1321 ctttccatga tttcctcctc gctgcagatc agcgtgcccg gcggctggtt ggcgtgcggg
     1381 ttgatatcat cgggcttgtc gaaactggaa cgaacgaaaa tcggcatgtt gtggaccatg
     1441 cccagttcca ccgaacggac ctgcagtacc ttggcgccct gcgacgccag ttccagcatg
     1501 tcctcgaacg cgattctgtc gagccggcgc gccttcggaa ccacccgcgg atcggtggta
     1561 tagacgccgt cgacgtcggt gtagatatcg cagcggtcgg catggatggc ggcggcgatc
     1621 gccaccgccg aggtgtcgga accaccccgc ccgagcgtgg ttatccgccc ggtctgcggg
     1681 ttgatgccct ggaagccggc gatgacggcg acctccttgc ggtccttgaa gcggttgatc
     1741 agttcggaac cgtcgatctc gagaatccgc gccgatgcgt gggcgtcgct ggtgcgaatc
     1801 gggatctgcc agccttgcca ggagcgcgcc tgaatgccga tcgcctgcag cgctatcgcc
     1861 agcagtcccg cggtgacctg ttcgccggac gccacaacgg cgtcatattc gcgcgcgtca
     1921 tgcatcggcg aggcgtcgcg gcaccattcg accagttcgt tggtcttgcc ggccatcgcc
     1981 gataccacca cggcaatgtc gtggccggca tcgacctcgc gcttcacatg ccgcgcgaca
     2041 ttgcggatgc ggtcgatatt ggcgacggat gtgccgccga atttcatcac gaggcggccc
     2101 atgacgactg gtgcattccc tcaagaggat aagtaatgtg acccgtacgg aaaccggagc
     2161 gcgcgggcca aaaggcgcgt atacatagcg gcgcggtcgg gggcacgcaa ccgggttccg
     2221 gctatcccgc cgaaagcggc gcattgtggt gttggggtaa ttgaggcaag cgtatggggc
     2281 gttatatcga cgatatcctg cagcccggtg aaaaggtgct gtattcgacc aatgcgcact
     2341 ggatctttta ttggccggcg atcgtggcct ggatcgtggc cgtcgtcctc ttttttctat
     2401 cgcgcgcgac cgtcaacgaa agcatcgtcc tgctttgcct gtcggcctcg gccgtcgtgg
     2461 cgatcgcggc gctgtactgg accttcaagg cctggttcca tcgctggacc accgagaccg
     2521 atgtcaccaa tctgcgcgtg gtccacaaga ccgggttcat caagcggcgc acgttcgaaa
     2581 tgagtctcga caaggtcgag agcgtcgatg tcaaccagag cattctcggc cgactgttga
     2641 actacggcaa cgtcacggtg cgaggcgtcg gcgagggggc cgagacgatc acgacgatcg
     2701 cttcgccgct cgacttccgc aatcacatca ccgcgcgata ggagcggcgc gcaatccatg
     2761 gccatgcaac aaaattctcc tgcaggtttg tccggcgccc cggcgtccac ggtcgacccc
     2821 gccgaaattg cgaagttctc gaaactgtcg gacgaatggt gggatcccaa cggcaagatg
     2881 gcgccgctgc acaagatcaa tccgctgcgg ctgacctata tccgcgacgc cgcctgccgc
     2941 aagttcgagc gcaacgtcaa aagcctgaac tgcctttccg gcctgcgtat cctcgacatc
     3001 ggctgcggcg ccggcctgtt gtgcgagccg ttcacccgcc tcggcgcgca ggtgatcggg
     3061 gtcgatccgt ccgccaccaa catcgcggcg gcgaagctgc acgccgacaa ggcgcagctg
     3121 tcgatcgatt atcgctgcac caccgtcgag cagatggacg tgcgcgagcg cttcgatatc
     3181 gtgctggcga tggaagtgat cgagcacgtc accgacgtcg gggtgtttct caatcgctgc
     3241 gcggcgatgc tcaagcccgg cgggctgatg gtgatctcga cgctgaatcg caactggaag
     3301 agctttgcgc tggccatcgt cggcgcggaa tatgtcttgc gctggctgcc gcgcggcacc
     3361 catcaatggg acaagttcgt cacccccgac gaactcgcgc atcatcttga gaacaacaag
     3421 ctcgccatca ccgagcaggc cggcgtggtc tacagcccgt tcgccgaccg ctggagcatc
     3481 tcctccgaca tggacgtgaa ctatatggtg gtggcggagg gggtttgagc tggaatttga
     3541 gggtggtacg ctaggttttt gtcgtccctg cgaacacgtt ccgcggggac gacgctgtgg
     3601 ttggtgccgc atgcaatcct gacggttgac cgcgcacagc cgcatcggca cggtagccgc
     3661 aatttcagcc ccgctaaccc tcgatcccca catgctcagc attgccacgc tctggattcc
     3721 cttcaccatc gtcgcggcgc tgggccaggt cgcgcgcaac gcgatgcagc ggcagttgac
     3781 ggcaccgctc ggcacctggg gcgcgaccaa tatccgcttt ctgttcggct ttccgttctc
     3841 gctgatcttc tttgccgtcg tgatcgcggc gaccggcgac gccgtgccgt ggccgacgcc
     3901 agcgttctgg ccgtggctgc tgctcggcgc gctcagccag atcgtcggca ccggcatgat
     3961 gctgctcgcc atgaacgacc gttcgttcct ggtgacgacg gcctatctga agaccgaggc
     4021 gatccagacc gcgatcttcg gttttgtctt tctcggcgat cacctgaccc tattgaaggt
     4081 gatcgcgatc ctgatcgcga ccgccggtgt cgtcattgcc gcgctgcggc cgggcgctgc
     4141 gaagggattt gcggatctga agccgacgct gctgggcctc gctgccgccg cggcattcgc
     4201 gctgtcggcg gtcggctttc gcggcgcggt tatcgtggtg cccggggtgt cgttcgtgac
     4261 ggcggcctcc tataccctgg tgttcggcct gttcgtgcag acgctggcgc tgtcgatcta
     4321 tcttttggtg cgcgcgcccg acgtgctcgc gaaaatcctg ggcttgtgga agccctcgtt
     4381 gttcgccggc ttcatggggg cgttcgcctc gcagttctgg tttctggcgt ttgcgctcac
     4441 ggcggccgcc aacgtgcgca cgctggcgct ggtcgaggtg ctgttcgcgc aagcggtggc
     4501 gtactattcg ttcaagcagc cgctgtcggg ccgcgaactc ggtggcatcg cgctgatcgt
     4561 gatcggcgtg gcgctgctgg tttctgttta gcgtttgatg acgatcagca cggcgccggc
     4621 ggcgatcagc gctatgccgg cagagaagca tcagccgtcg ttccggggcg cgagcaaagc
     4681 gagcgaaccc ggaatctcga gattccgggt ccacgcgaag acgcgtgtcc cggaatgacg
     4741 aaagacgtca attcctgtcg tccggcagta ccggcaacgg cgagacttcg acgccctctt
     4801 cgatcagggc gcgcgcttcg tccggtgacg cttcgccgta gatcggacgg tgctcgatat
     4861 cgccatagtg cattttccgc gcttcgttgg gaaagcgctc gccgacattg tcggcgtttt
     4921 tgacgatatg atcgcgcagt tcccgcagct tggcgcgcag ctcgcgttcc tgtgccatca
     4981 gcaggggcgt cgacgttgga gtggtgacat ccgtgggcgt gggcgcggca ggcgccggta
     5041 ccgcgctatc ccggcccttc ttgctcacga tctgcggggc catgatggcc cgctcgacct
     5101 tggcggaacc gcagacggga cagttcacca gcttgcgctt ctcctgggtt tcgtaggccg
     5161 aggagctctg aaaccagctt tcgaacgcgt ggccgcgctc gcagcgtagg gagtatcgga
     5221 tcatgcggag ccccgcacca ggtgcagatg ctcgggaccg gctttgggat cggcgacgct
     5281 gaagcgccgg ccgtgctgca gcgacggcac ggtcttgcgc gcggtctcga ccttggccgg
     5341 atcaatcttc gccataaaaa cgccgggctc gacgccacct tcggcgagca cctcgcccca
     5401 gggagcgacg atcagcgagt gaccataggt ctcgcgcttg ttctcgtgca tgccaccttg
     5461 cgccgcggcg aacacgaagc agccattctc gatggcgcgg gcgcgcagca gcgtgtgcca
     5521 atgcgcttca ccggttttgc gggtgaaggc ggaaggcacc gtgaggaacg cggcgccgct
     5581 ttcggcgagc gcgcgataca gcgccggaaa gcgcacgtcg tagcagatcg tgagcccgat
     5641 ccggccccag ggcaggtcgg atatcacggc ggtctcgccg ggctggtagt tggcggactc
     5701 ccgatagctc tcgccgccgg gcagatcgat gtcgaacatg tggatcttgt cgtagctggc
     5761 gatcaccagg ccatccggtc cgatcaggaa cgagcggttg accgccctct cgggcgaagc
     5821 acgcagcgcc agcgagccga catggagatg gatcttcagt tccgccgcga gcgcgcgata
     5881 ggctttcagc gaaagatcgt cttcttcggc ggcaagatgc tcgaacagcg cggtgcggtt
     5941 cagctgcatc atgttgctca cctccggcgt gagcacatag tccgcgccct ccgcggcggc
     6001 ctgccgaatg agcttggtgc cctgttcaag gctcggctcg ggcaacagcc ctgtgcgcat
     6061 ctgcaccatc gcggcggtaa agctgaggtc agcgctcatg aattggcctt ttcgcctgcc
     6121 agcatcggat cgagcttgcc cgcgcggtcg agggcataaa gctcgtcgca accgccgaca
     6181 tgggtctcgc cgatgaaaat ctgcggaaaa gtcgagcccg cgccggcgcg atcccacatc
     6241 cgctgacgaa gcgtcgaatc gacagcgaca tcgtgttcgg tgaacgcggc ttttttgcgc
     6301 gtcagcagtg atttggctgc actgcaatag ccgcagcccg gacgggtgaa gatttcgatg
     6361 gcagcggtca tgttgcgctc tgctttggaa tgttacccga ttatatggga gctttgtggg
     6421 tgtcgacaac ccgggcgaac accagcacat cgacggaggc cgccttggcg cgcaacagcg
     6481 cccgcgcgca ggcatcgacg gtcgcgcccg aggtcaggac atcgtcgatc aggatgacgc
     6541 gacggccctg aatctcggac tgccggctgg cttcgacctt gaacgcgccc tgtacgttgc
     6601 tggcgcgctg cgacctcgac aggccgatct gctgctgggt cggccgcacc cgccgcagcg
     6661 cttcggtagt cactttcacc ccgctttgcc gttcgatgat gcgcgccagc gcgccggact
     6721 ggttgtagcg gcggctccag ccgcgtcgcc agtgcagcgg caccggcacc agcgcgtcgg
     6781 cctcgccgag cagttcacgg ccggcccgcg ccatccagcg gcccatcgcc ggcgcgagat
     6841 cagtgcggtc ctggtatttc agcgcatgca ccagcgtgcg ggccacgtcg tcatagcgca
     6901 ccgcggcgcg agcgcgcgca taggccggcg gatcggcgat cgcttccatc gacagcaggc
     6961 cgggaccggg atcgtaaaca aagggaatgc cgagccgcgg gcaataaggc tgtgcgatga
     7021 acgacagctt ggcccagcag gttgcgcaga cgccctcgcc agctaccggc tcgcggcagg
     7081 cgacgcacag cgtcggcagc gcgatgtcga gcgccagccg cgccgcatga gcccacgcac
     7141 cgcgacacgt gttcagtgcg ccgcgcagat gggtggagat ggaacgggat gacgaggttt
     7201 cggcgtccat gcggggaagg gtagcgccgc gccgcgtggc gctcaagtcg gacgaacgga
     7261 atttcctcgt cattgcgagc caacgggtcg cgcgaatgcg cgcccgatga caggctccgc
     7321 gaagcaatcc atctggttgt ggcctatcgc ttccatcatc cgcgtctctg gaatactgga
     7381 tcgcccgcct tcgcgggcga tgatggtctc cgccgtcatt gcgagcgaag cgaagcaatc
     7441 catcttgcag cgaaaagaaa gagtggattg cttcgctgcg ctcgcaatga cgttgataga
     7501 gtcccgcata ccacttcgtt ctcgcgacgc ggagcgcccg aggcgttgct attaccttcg
     7561 cgcccaaagg gcgtggggaa cgccgggtgc ccgctgcacc cgcggcctcg tgtgcacttg
     7621 tagtggtaga aagcacacga gtaacaacga gcacaccgga atcacccgac gttcccgcac
     7681 gcaatggttt tacgacttat ttcgtgctct ccccggtgac cgggctcttt tgccaccgtc
     7741 atttgcggat taaggttttg tccaagcccg gtagggccga ctcaacctcc gcgaacttga
     7801 cgccagcgtc ggggcgtcag gaccacacga cttcgccgta cgctgcaaca tctctcgtcc
     7861 gcgtgctgtt gaccgctcac ggattcaaag aacccgccct gcgatcccgt cgcacgcaaa
     7921 acgctgccgc gtccaccgca tcccgtgccc tacgtccgtg acgatcgcga aacgcccctc
     7981 tgtgtgggac gggatgcaaa aagttctaga tgtgatttgg ggggtacgaa aacagaaata
     8041 tttttgcaaa gggggctgga cacaaaactg cccgacgggc aaatcacgcc gtcttcttca
     8101 tcaatagcgc accttcgagg aactgcgcgc gctcccgtaa gcggcctagg ctccggccct
     8161 acaatcaagc cccggctaat caccggggct ttttctttgc gctctcgcgg atagcccagc
     8221 tctcacggag aaccacgcgg acagcctcgc ctaatggttt gtttaccggg ctcgacgaca
     8281 gttgccccgg gccgcgtatc atgcctaagt cgtcgccgat gtctcaaccc ggcggcggca
     8341 gcacgccgag ccaagcgccg cgccagacaa gcccgcaggc ccatcgtata cggtccgggc
     8401 tggggccgat ggaccatgag caacgttcgg cgcgatctcc gcagtggcgc gctcagaacc
     8461 cacgccaacg gcgagaccat ccattgatcc cggaaggggc ttgcgcgctc aacggtcttt
     8521 gctacgcgag agacagatca atcaatcttg gcgacaaaat ttatattttc attcttggaa
     8581 taggaggacg tgatgagatc agcccttaca atattggtcg cgctacttgt gacaatgcca
     8641 gcgatggcgg aacagccgaa caagccaatc aagattgcgg ggatgtgcgt actactgggc
     8701 gaaaagactt cgggcgctta taagttctgc aactacaatt gccctgcggg tacgtacaca
     8761 atcgaggtca agccgctgga aagatgccct gtaacaatcg atcactaacc tgggccttta
     8821 gtggttacaa gcgaatgttc gttctaagac gacgagtatg cctagatcag tcgcgaccgt
     8881 tccgagcgga ggaatttttt gtacttcgcg acgaagcatg tcgagtacct gatcgcccgt
     8941 tagaactaaa ttcgagggcg gccagtagag tggggtctgc ttcttcccga cgattgcgcg
     9001 atttgctgcc gatattcccg tttcgatgag gtcagcatgt atccttgcgt attgtttttc
     9061 caaagggtcc ttgctgtcat aggctttgag caggccgtca atgtcgccct ccgcttttgt
     9121 ggggtttgaa gaggccaaca aaacacaggc ggccaacgct atgcatgcca gcccgatggc
     9181 tgcgatcttt gcgcgacgca tcgctttccc cggtcccatg ctcgcaagcg agcgcgggct
     9241 ccatttccct ataggtagcg tctcataaag tgatcgcgcc gacaatggat acgcctgaaa
     9301 gaacctgaat aaattcgctg actgacaggc cagtcgctgg tacgttttcg ccacccatgg
     9361 agggaatggc tcaatgcggt tcgaagtcgg tcagactttt cagggcacag tagacatcag
     9421 catgcgcgcc gaagtgatcg aggtaagcaa cgatggccgc gacgccaagc tcaaattgct
     9481 aactcacgac ggcagcgcgt tcgaactcaa tgccggcgca gtcatggccg gtcatcagaa
     9541 gtggcgcttg gtgccgtaaa acggatgaga cccggagccg atcaagaggc aggatcaagc
     9601 cgagagggaa ataccatgat ggctgcgaaa gtgacttctg cactaattaa gtcggtccaa
     9661 acaaatcccg accccttcat gacgtacgga gattttgggc gagcgtttgg gctcagccaa
     9721 aaagtcactg taagctgggc aaacaggggc acgctcgatg ctgttgcatc tgccctaaag
     9781 attgatccga aggttgcgat tgacctaacg ttcctacttc gaagcaaagc gggcggcttc
     9841 cccatggtga tcgacggcct gccgtatgtg ccggacgaca aggtacagga gcagagagcg
     9901 cgagacgtgg ccgatcaaat catcgcacag ttcaagctta cggcgaaaaa cccttactaa
     9961 cccaacagtc gcgacgctga tgatccgcct tgtccgttct gcacggcacc gcttcacata
    10021 tacactctca aggggtattc tgggcgtggc agttagcggg agtttccaac tgcatgccga
    10081 ttttagttga tccgcagatg gttgcggacg atagcggaag cgagccgcaa agtcccattt
    10141 tcgttctagc aggcttcatc gctccagcgg cgaagtgggc cgatttcgcc gtggaatggg
    10201 aagctgcgct caatctgtcg ccgaaactcg actatttcaa aatgactgag gcagccaatc
    10261 tttcaggtca attttcacgc cgaaagggtt gggatgaggc caagcgtgat gatcgcctcg
    10321 ttacgctaac gcgcattatt cgaaagtacg ccagcgttcg ggtttcggct tggataatgc
    10381 atgatgacta cgacacgcac atcaaaagcc tcaaggcccc tattcgtcat ctcgcccttg
    10441 atagccctta cgtcatgctc ttgcagcagc ttgtccttgc catcgccgtt tttggagatc
    10501 ggcacggcat caaagatcac tgcgattaca ttttcgacga gcaagggaaa ctagccgaag
    10561 aagcaacgta ctggtggccg cagttcaaat ggcatgtagg ccactcaagt cgcggcgatt
    10621 tggctaaatt cgttggttcg ccaccgatct ttcgagatga gaagagcttt ctgcctttgc
    10681 aggctggcga tctgtatgct tggcaaattc gcaatcaata cgccagaaat cacgccgtgc
    10741 cgaaccaaac gatctttgtt ccgcatagcc ggattttggg aatgctggat accattcctc
    10801 tcataaatcg agagtaccca acagatgaag tgctgcgatt gcgggagcat cttatcaacg
    10861 ccggagaaca gtatgctaag gcttttccag acgttcctct gattggtccg cacgcagatc
    10921 ggaaggaccg acgcaaggcg cacaaacaag ctagaaaagc gcgagctgtt tccaatccac
    10981 caacttcgtg acaacgggcc gatacttggc cggtgcgaac ctcgttggcc gagtttttcg
    11041 gctgccctaa ctttttcttc acatgatgtg gcgacattgg caggcgggtt gcgtatcaag
    11101 cccgctgttc tggaaagccg tcgccgatct caacccggcg gcggccgttt tttttgctat
    11161 gccgctggct gatcgtcttg aaatgatgtc gcgtttggaa aaacaacttt tgccgacgcc
    11221 aacgctgcgt cgattgtgcc aagcgtgatc ttttgaaccc ataggtccaa ctccgttcga
    11281 ctagggtcat ctgccgcgac gatatgcttc gcaagctgca acgccgttat gaagcattcc
    11341 gctacggcct ccgcgttcgc gggagcgata agaggaatgg cgctagcgtt gaccgcggat
    11401 ggcgaatttg tcttcatctg cattgcgagc aacatcgcct tggcttcggc aatccgcatg
    11461 tagaattcga tagtcgcttg tggatgcggg aaaagcccaa ttctgtccgc gaccgcagga
    11521 tagatgatgg gttcaacaag acttttcgct acatagactg catcttgcat cggaacggct
    11581 aagcctttgg tcgcgatttg ctcacagata ttgagcgcgc caatgacatg cttacaatag
    11641 gtcgtcactt caatccgcac cgcagagcag actttcgcga tatcttccct tcgttggcgg
    11701 gttagcgtca tgtacaccgc gtagacggcc ccgcccgccc caatcattcc accgataatt
    11761 gcgccgagcg cagcggcctg ctcgcccgac gtggctacga gaagaacgca ccatagccaa
    11821 cccaaaaggc caaggcccaa aatcagcaac acgccagcga tgttgttatg gcgatcatcg
    11881 ttcgtcattg gcgcgcgccc catccccgtg ctgcatgtac gctgcccgat tgaatgggtc
    11941 aatgcacgac gcgcggcgca caacgtaggc tgtcgatgtc gatgcaaaac ctcatcatgc
    12001 ttcgttgcac agatcgcggc gcgctccgga taatcaacgc atagagcgcg cccgccgcga
    12061 cctcatcgcc gccgacagtc tcatcaaacc gttcggcgag catagagcag cggtcccaat
    12121 gcgcgagcgc gcaagcatca tgatggctca tcgccgagcc ctcgcacgtt ttgcacgccg
    12181 aggcgcgggc aggggcgggg caacccgggt atcagttaga agcttgtcga gatcagagac
    12241 gcggatcaca acgcgcccgc gcttgcccgg caggccaatg cgagagaacg gcaagcgccc
    12301 gctgtcgagt agcgcacgca tagttgtctg gccgacgccg acgcgctggc aagcttctga
    12361 gatcgataca gacaggatgg tttcggaagg gagagtttca ggcatagcac ggctcttcga
    12421 gtgtaccgga agccccctcc cggcgaacta gattgcgtgc tgcatgagcc ctccatttag
    12481 cagagtgcga gcgcggttca aaacgatatc cccggggcta ggcggggcta tatgatgtcc
    12541 agcgaactat ggaaaaatct attcgatcca accaaaaacc caataaaaaa tggcataact
    12601 tgaacatttg ttgcggcggc tccagccgcg ccgccagtgc agcggtaccg gtaccagcgc
    12661 gtcggcgtcg ccgagcagtt cacggccggc ccgcgccatc cagcggccca tcgccggcgc
    12721 cagatcggtg cggtcctggt atttcagcgc gtgcaccagc gtccgcgcca gcgcgttcac
    12781 gcgcgtcttc gacgcgctat ggcttgctcc gtggtgcgct gcagatccgg ggttcatgaa
    12841 acttccctcc gcatgggtcc cggctctgcg gagcagcgcc gaagaggcgc tgcaccgcgt
    12901 ccgggacacg atatgctgtg attgccaccg accggccgac cggcgtacca atcggcatgg
    12961 ctccgaaccc gagcaccgcg cccatcctgt tcgaccgcgc cttgttgcgg gcgcggcagt
    13021 cgcgcgcatt gcgatcaggg tccgcaacat tcctgctcga tcgcgtcgcc gaggatcttg
    13081 ctgaacgatt gcatgcggtg cttcgggaat tcaagacggc ggccgatatc ggcaccgtgg
    13141 gcgatcaggt ccgcgacacg gtggccggtc gcgtagacac aatcgcgcgc atcgaactgc
    13201 cggaccggga gtccgaaccg attccgctgg cgccggaatc gcttgacctt gcggtttccg
    13261 cgctggcgtt ccagttcgtc aacgatctgc ccggcgtgct gacgcagatc cgccgcgcgc
    13321 tgaaacccga cgggctgttg ctggcggcga tgatcggcgg cgatacgctg accgagctgc
    13381 gacaatcgtt tgcagctgcg gaagccgagc tcgaaggcgg ggtgtcgccc cgggtggcgc
    13441 cgtttgccga tctgcgcgat gtcggcgcgc tgctgcagcg tgcgggtttt gctctgccgg
    13501 tcaccgacgt cgaccgcatc gtggtgcgct atgacagcgc cttcgcattg atgcaggatc
    13561 tcaggcggat gagcgcgacc aatattctgg tcgagcggcg gcggacgcca acccggcgcg
    13621 ccacgctgct gcggatggcg cagttttacg ccgagcgctt tgccgattcc gatggccgca
    13681 tccgcgccac cttcgatgtg atctggctct caggctgggc gccgcatgac agccagcaga
    13741 agccgctgcg gccgggatcg gcgaaagcga gtttggcgga ggcggtgaaa aaagttgccc
    13801 caaagtaacc agtgtcgttg ccgggcttga cccggcaatc catcgtgctt cgcatgaatc
    13861 tgttcgaagg ttgatggatg cgcgggtcaa gcccgcgcac gacgacggta gagcgatgcg
    13921 acgcccgtcc tcacatcaac aggtcgatca gatgcgggat cagcggaatg tccgccggcg
    13981 gcatcgggta gtcgcgcagt ttgttggcgc ggacccaggc gagtttctgg ccctcgcgcg
    14041 cggtgacctg gccttcccag cgccggcaga tgtagagcgg catcagcagg tgaaaatcat
    14101 cataggcgtg gctggcgaag gtgagcggcg ccaggcacgc ctcgctcacg gtgatgccga
    14161 tttcctcgtg cagttcgcgg atcagtgtcg cttccggccg ctcgccgggt tcgagcttgc
    14221 cgccgggaaa ttcccacaag ccggcaagcg ttttgccttc gggccgctgc gcgatcagca
    14281 cgcgcttgtc ggcgtcgatc agcgcgcagg cgaccacgag ggtgagtttg aagtcaggca
    14341 tgcgtggtga atgctctctg ggatgctttt ggaatcggtc tcgacgattg atcgaaccgt
    14401 ttggaaggat ttcattaact ttattccgcc tgcaattcta cggtcgcgat aagtgatact
    14461 taatccgccg ctgctaggtt ttgcacaact caaaccggca tcgcggcacc cccatgcata
    14521 ccttttttcg cttgattggc cgtttccgca gggataagcg cggcaatctc gcggtgatct
    14581 tcgcggtcgc ctgcgtcccc ctgatatcgg cgatcggttg cgcggtcgat tatacccggg
    14641 cggtcacaat tcgaagcaaa ctgcaggctg ccgtcgacgc tgccagcgtc ggatcggtcg
    14701 cgagactatc gccggcgtcc actgccgccg caacgatgag ctcggacgga ccgattcagg
    14761 ccggcgtgga cgatgcaacc aagatcttca acggcaatgt gtcgggaatg accggctata
    14821 cgctgaacag ctttaccccg agcgtgacca agtccggcgt aaagattgtc tccgacattc
    14881 ggttctccgc cgatgtcccg accatgtttc tcggcgtgat cggcaagagc aagatgaccg
    14941 tcaccggaga ctcaggcgcg gttaactcgc tgccgccgtt tctcgatttt tatctgatgc
    15001 tggacgtttc ggggtcgatg ggtttgccgt cgaccaacgc cgaacagacg cggctttccc
    15061 agatcagtcc ggatatgttt gtgcagtatc ccggcggctg caccttggcc tgccacttca
    15121 ctgtctcaaa ttcgtgcgcg aacggcaatc agaaataccc caccaacaac gaatgtctcg
    15181 gctattggat atcgcgcgtc agcccgaccg cgtacgcgaa cctcgtcagc agcccgaccg
    15241 catggaacgc ggcggggact tctctagtgt cgaagccggt tcctggcttg ccgaattcga
    15301 tgatttccgg cctgccaaat tcgttgagtt ccggcgaccc taacaggctg ccggcggttt
    15361 catcctgcgc gaccgacggc accgccaact gcatccagtt gcgcgccgac gcggtcggct
    15421 acgccgtgaa ccaattgttc atcacggcta acgaccccac ctaccagaag gtgcccgggc
    15481 aattccggat cgggctctac c
//
LOCUS       DASWGM010000611         4746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1568089,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000611 DASWGM010000000
VERSION     DASWGM010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568089"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VGA15_17330"
     CDS             <1..521
                     /locus_tag="VGA15_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006699683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9589496.1"
                     /translation="GWEKVSQVRKPIVAAVAGYALGGGCEMAMMCDFIIAADNARFGQ
                     PEIKLGIIPGIGGTQRLTRAVGKAKAMDLILTGRMMDAAEAERAGLVARVVPAASLME
                     EAMKVAETIAAMSLPSVLSAKEAVNRAFESPLAEGLRFERRVFHALFATEDQKEGMAA
                     FVEKRPPKFRNR"
     gene            669..2258
                     /locus_tag="VGA15_17335"
     CDS             669..2258
                     /locus_tag="VGA15_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9589497.1"
                     /translation="MSPATDLDSLSHADLKSLVLKLLEEMAELRRMVAAQRDEIARLK
                     GGSGRPNIKPSGMDKATEPKPPPPAGGEPGRKGSKTSKLSIHEERTVKVAAPPQGSRF
                     KGYANYLVQDLVIRPHVVNFRCERWQTPDGNTMTAPLPAGISGHFGPELRRFVLAQYH
                     QGQVTVPRLLALLRALGIHISKRQLVRLLIAGQDGFLAESRDVLRAGLSSAPWITVDD
                     TGARHKAANGFCTQIGNAHFAWFGTTDSKSRLNFLELLRAGHGDYVINTEALAYMRHR
                     SLASPVIARLAEHLDQLFADQSAWSAHLDRLGISALKVTPDPIMVATEGALWGSVKAH
                     GFLPNTVIVSDDAGQFNVGRHGLCWVHAERLIHKLDTFTDEQRDAQSSTRALIWRLYR
                     DLKAYRLHPTKRRKAALRARFDRIFTRRTGFVTLDRLLARLNANKTELLMVLDRPEIP
                     LNTNGSENDVRCQVTKRKVSGGTRSDTGRDCRDAFLGLSKTCAKLGIAFWDYLGSRLA
                     VPNHPDIPYLPTIVRHRCATA"
     gene            2692..3435
                     /locus_tag="VGA15_17340"
     CDS             2692..3435
                     /locus_tag="VGA15_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589498.1"
                     /translation="MIKIEADHRLAWYRSHFNPDQPRVPAGHPDGGQWTRTGGYEGSV
                     EAARTRPTQSPSESVTDEQPDPIRPGARYAQAKTQIVIGPRALTGIARIDEITKALAE
                     TLARAVDTVEYLPTLTPRLYGTLVHLAFANMVRFQRLPGIGFWDVETTFSLEPGAHYG
                     SKDSIRTDVVLRNEVGDIIAIYDVKTGNQGLRPGRVNELRANAQVSPSVPVIELHVLH
                     GVRLTREQIGPSLGTRQAWLLPQINVSGV"
     gene            3455..3904
                     /locus_tag="VGA15_17345"
     CDS             3455..3904
                     /locus_tag="VGA15_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589499.1"
                     /translation="MLSDRRLKSIADWQAAISAEGFPVSLTTGVPLEELNGIFPVQLG
                     GQLTDFECVHWRGPEVMAEMADVDFGHPWVHVLALRWGADLYAGPAAYMAAAAYAKAA
                     GGVLFDCEEGKIITPKQAARIGRELEQAIPEAQAFLRRLMEKLKHQP"
     gene            3994..4428
                     /locus_tag="VGA15_17350"
     CDS             3994..4428
                     /locus_tag="VGA15_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589500.1"
                     /translation="MASDIREEQEREWTELHDRVRAVLQHYGEEDVGGERRGYHLVDD
                     NLGFHRHKIETEELKLIMPIVVKSLQELLSGYPNWEIVMAVASPDKETRPNMTLVIRD
                     DEIIDGLQRQCLPAEFRTIQYEGSRPLGSKLGDVIYSGPGPF"
BASE COUNT          954 a         1435 c         1477 g          880 t
ORIGIN      
        1 tcggttggga gaaggtgagc caggtgcgca agcccatcgt ggccgcggtg gcaggttatg
       61 cgctgggcgg cggctgcgag atggccatga tgtgcgactt catcatcgct gccgacaatg
      121 cccgcttcgg ccagcccgag atcaagctcg gtatcattcc gggcatcggc ggcacccagc
      181 ggctcacccg cgcggtcggc aaggccaagg ccatggacct gatcctgacc ggccgcatga
      241 tggacgcggc ggaggcggag cgcgccgggc tggtcgcgcg cgtggtgccg gcggcaagcc
      301 tgatggagga ggcgatgaag gtcgcggaga cgatcgccgc gatgtcgctg ccgtcggtgc
      361 tctccgcgaa agaggcggtc aaccgcgcct tcgagtcgcc gctcgccgag ggtttgcgct
      421 tcgagcggcg cgtgttccac gcgctgttcg ccacggagga tcagaaggag ggcatggcgg
      481 cgttcgttga gaaacgcccg ccgaaattca ggaatcggta accgatttgg gttttccgaa
      541 tttccggtta tatgaccgga acgactgcta gagcaggaac ctatggccag caaacgtaag
      601 aggggcaaaa cccgtggcgg tatgatttta cgattctcag gacggcgcga gtcagattca
      661 ttgtgggaat gtcgcctgct acagacctcg atagcctttc gcacgccgat ctgaagagcc
      721 tggtattgaa gctcttggaa gagatggccg agttgcggcg gatggttgcg gcgcaacgcg
      781 acgagattgc ccggctcaaa ggtgggtcag gccggccgaa tatcaaaccc agtggcatgg
      841 acaaggcgac cgagccaaag ccgccgccgc cggctggagg cgagccgggc cggaagggca
      901 gcaagacgtc gaagctgtcg atccatgagg aacgcaccgt caaagtcgcc gcgccgccac
      961 agggatcacg cttcaaaggc tatgcgaact atctggtgca ggacttggtg atccgcccgc
     1021 acgttgtgaa tttccgctgc gagcgttggc agacgccgga cggcaacacg atgacagcgc
     1081 cgctgccggc cggcatcagc gggcattttg ggccggaact gcgccgcttc gtgctcgccc
     1141 aataccatca gggccaagtg acggtgcccc ggctgttggc gctgttgcgg gcgctcggca
     1201 tccacatctc caagcgccag ctcgtccgcc tgctgatcgc cggccaggac ggctttcttg
     1261 cagagagccg ggatgtgctg cgcgccgggc tttcgagcgc tccctggatc acggtggatg
     1321 acaccggcgc ccgccacaag gccgcgaacg gcttctgcac gcagatcggc aacgcacact
     1381 tcgcctggtt cggcaccacg gattcgaaga gccgtctcaa tttcctggag ctgctgcgcg
     1441 ctggccatgg cgattacgtc atcaataccg aggcgctggc ctatatgcgc catcgctcgc
     1501 ttgcctcacc ggtgatcgcc cgcctggccg agcatctgga tcagctcttc gccgatcagt
     1561 cggcctggag tgcacatctc gatcggctcg gcatctccgc gctcaaggtc actcccgatc
     1621 cgatcatggt cgccaccgaa ggggcgctgt ggggcagcgt caaggcgcat ggctttctgc
     1681 ccaataccgt gatcgtgagt gacgacgccg gccagttcaa tgtcggccga catggcctat
     1741 gctgggtcca tgccgagcgc ctgatccaca agctcgacac ctttaccgac gagcagcgtg
     1801 acgcccagag cagcacccgt gcactgatct ggcggctcta ccgcgatctc aaggcctatc
     1861 gccttcatcc gaccaagcgg cgcaaggccg ctctgcgggc gcgcttcgat cgcatcttca
     1921 cgcgtcgcac cggcttcgtc accctcgacc gtctgctcgc tcggctaaac gccaacaaga
     1981 ccgagttgct gatggtgctc gatcggcccg aaatcccgct gaacaccaat ggctcggaga
     2041 acgacgtccg ctgccaagtc accaaacgca aggtcagcgg cggcacaaga agcgacaccg
     2101 gtcgcgactg ccgcgatgcc ttcctcggcc tcagcaagac ctgcgcaaag ctcggcatcg
     2161 ccttctggga ttatctcgga tcacggctcg cagtgccaaa ccaccccgac atcccatact
     2221 tgccgacaat cgtcaggcac cgctgcgcca ccgcctgaca gccccgactt ttgcccctct
     2281 tacgaagaac ccccatagcg ccttgatgcg aaacgacaaa atgcgtgtgg ctctgtgcgt
     2341 cgggcatgaa ttctcgcgtc gcgtgcctcg tgcacgcaat tttttacttg acaatgtaac
     2401 gaaaatcagg tacaagagga gcataatcca caaatgcgca ttgaccaccg tttcggtgcc
     2461 gcctcggtga acccgaggcc ggcatcgacg cgggaggatc gcttttgcgg cgcgcgttcc
     2521 gcgccgtgac gagccttccg ctcaaccacg gacacgacag ccgatccgcg ccgcaatcgc
     2581 ggggacggct cgtgcgcatc gcaggcatcg acaatgactc gcttctcctc ggacaccgac
     2641 cgtctgctgc tcgatgccaa caatgccgcc gcgctcgcgc ataagctccg attgatcaag
     2701 atcgaggccg accatcgcct cgcttggtac cggtcgcatt tcaatcccga ccaacctcgc
     2761 gtgcctgcag gtcatcccga cggtgggcag tggacgcgca cgggcggtta cgaaggatct
     2821 gttgaagctg cacgaacgcg accgacccaa tccccgtcgg agtcggtgac cgatgagcag
     2881 ccggatccca taaggccggg tgcccggtac gcgcaagcca aaacgcagat cgtcatcggt
     2941 ccgcgcgccc tgaccggaat tgcgcgcatc gacgagatca cgaaggcact cgccgagacg
     3001 ctcgctcgag ccgtggacac ggtcgaatat ttgccgacgc tcaccccgcg actgtatgga
     3061 acgttggttc atctggcgtt tgcaaacatg gtgcgcttcc aacgactgcc gggaatagga
     3121 ttctgggatg tcgagacgac tttcagcctg gagccggggg cgcactacgg ctcgaaggac
     3181 agcatcagaa cggatgtggt cttgcgcaac gaagtcggag acatcatcgc catctacgac
     3241 gtcaagaccg gaaaccaagg attgaggccg gggcgagtga atgaacttcg cgccaatgct
     3301 caggtatcac caagcgtccc tgtgatcgaa ttgcatgtcc tgcatggagt gcgattgacc
     3361 cgtgaacaaa tcggtcccag tcttggcact cggcaggcgt ggctgttgcc gcaaatcaac
     3421 gtcagcggtg tttgaaatgt cgatggagct ctacgtgctc tctgatcgac ggctaaaatc
     3481 catcgccgac tggcaagctg ctatttctgc ggaaggattt cccgtcagtc tgaccaccgg
     3541 cgtcccactt gaggaattga atgggatatt tccggtgcaa ctgggcggcc agctgaccga
     3601 cttcgaatgc gtgcactggc gcgggcccga ggtgatggcg gaaatggccg atgtcgactt
     3661 tggccatcca tgggtgcacg ttctggcgtt gcgctggggt gctgatcttt atgccgggcc
     3721 ggccgcctac atggccgcgg cggcatacgc caaggcggcg ggcggagttt tgttcgattg
     3781 cgaggaaggg aagatcatca cgccaaagca ggcggcaagg atcggtcgcg agctcgagca
     3841 ggcaattccg gaggcgcagg catttttgcg gcgcctcatg gagaagctca aacatcagcc
     3901 ctgacctcgc ttatcgcgaa aatgataaga ccaatgcgga tgtcttaccg acctcacgcc
     3961 ggcgcccgcg gcagcgaata ggaactcgga agcgtggcca gcgatatccg ggaggagcaa
     4021 gagcgggaat ggaccgagct ccatgatcgg gttcgcgcag tgcttcaaca ctatggcgag
     4081 gaggatgtcg gtggtgagcg gcgaggttat catctggtcg atgacaatct tggcttccac
     4141 cggcacaaga tcgagacgga agagctgaaa ttgataatgc cgattgtcgt caagtccctg
     4201 caggagctgc tgagcggata tccgaattgg gaaatcgtga tggcggtcgc cagtcccgac
     4261 aaggaaacgc gtcccaacat gacgctggtc attcgcgatg acgagatcat cgacggactg
     4321 cagcggcaat gtcttcccgc cgagtttcgg acgatccaat atgagggaag ccggccgctg
     4381 ggatcgaaac tcggcgacgt catatattcg gggccgggcc ccttttgatt tcggccgctt
     4441 cgatcggtgc aatggtgccg tttcacggaa atgtaagtcg ctccaaaggg ttcaagaatt
     4501 tcgcttggtt ctaaaaattt cacttgacaa tgtaacgaaa atcaggtaca aggggcgcat
     4561 aatccaaaca tgcgcattgg ccaccgtttc ggtgccgccg cggtaaatcc gagggcagca
     4621 tcgacgcggg aggatcgttt tgcgccgtct gttggtggct gcggcgatcc tcccgcgaat
     4681 ccttagctga cgccgcgaat gccggaatcc tccggccggg gcagggcctt gacgatgatt
     4741 cgtcat
//
LOCUS       DASWGM010000612         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_76281,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000612 DASWGM010000000
VERSION     DASWGM010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76281"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="VGA15_17355"
     CDS             complement(<1..1052)
                     /locus_tag="VGA15_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589501.1"
                     /translation="MTMKSLLSTVSLTLLIGGASATAQAQIAIGHLADYSGPTSDVGT
                     PYGQAVADTFAWVNKNGGVGGKQLNLDTNDYGYQVPRAIALYKKWSGADKVAAIMGWG
                     TADTEALTGFLAQDKIPDISASYAAALTDPEGSSGKAKPAPYNFFYGPSYSDAMRAML
                     TWAAEDWKAKGKPGKPKFVHMGANHPYPNAPKAAGEALATELGFEVLPPLVFALAPGD
                     YSAQCLSLKSAGANYAYLGNTAASNISVMKACKTAGVDIQFLSNVWGMDENAAKAAGD
                     AADGVIFPLRTAVGWGGNAPGMKTVMEISKMSDPTGKVYRPVHYIAAVCTALYMKEAI
                     DWAAKNGGPTGENVAKG"
     gene            complement(1109..>2001)
                     /locus_tag="VGA15_17360"
     CDS             complement(1109..>2001)
                     /locus_tag="VGA15_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9589502.1"
                     /translation="IAALGLNILVGFTGQISIGHAAFFLFGAFTSAYISNTLPIPVFF
                     AIPLAGVVTALVGLIFGVPAARLKGLYLVIATLAAQYILLDFFSRADWFSGGSVPASA
                     NPFSIFGYTFRGDRQYFYVVLAYVLVSYLLVTNLMRTRDGRALVAIRDHYLSAEIMGI
                     NLTKYRTLSFGLAAFFAGIAGALYAHYQLVVSNEGFGIERSIIFLAMVIIGGTGSIMG
                     TLMGTAFVVLLPESMEWISASLKGSVIDKALSLNNNITFLREIAIGLIIIAFLMFEPD
                     GLAHRWRQIKTYWKLYPFSH"
BASE COUNT          388 a          584 c          642 g          387 t
ORIGIN      
        1 cctttggcga cgttctctcc ggtcgggccg ccgtttttgg cggcccagtc gatggcttcc
       61 ttcatgtaca gcgcggtgca gacggccgcg atatagtgca cggggcggta gaccttgccg
      121 gtcgggtcgg acatcttgga aatttccatc acggttttca tgcccggcgc gttgccgccc
      181 cagccgaccg cggttcgtag cggaaagatc acgccgtcgg cggcatcgcc cgccgccttg
      241 gcggcgttct catccatgcc ccagacgttg ctgaggaact gaatgtcgac gcccgccgtc
      301 ttgcaggctt tcatgaccga gatattggag gccgcggtgt tgccgaggta ggcataattg
      361 gcgccggcgc tcttcaagct caggcactgg gcgctgtagt cacccggcgc cagcgcgaac
      421 accagcggcg gcagcacttc gaagccgagt tcggtggcga gcgcttcgcc ggccgccttc
      481 ggggcgttgg gataggggtg gttggcgccc atatgcacga atttcggttt tcccggcttg
      541 cccttggcct tccagtcctc ggccgcccag gtcagcatgg cccgcatggc gtccgaatag
      601 ctcggcccgt aaaagaagtt gtagggagca ggtttggcct tgccgctgct gccttccgga
      661 tcggtgagcg ctgcggcgta ggaagctgag atatccggaa tcttgtcctg tgcgaggaag
      721 ccggtcagcg cctcggtatc cgcggtgccc cagcccatga tcgctgcgac cttatcggcg
      781 ccggaccatt tcttgtagag cgcgatcgcg cgcggcacct gatagccgta atcgttggtg
      841 tcgagattga gctgcttgcc gccgacgccg ccgttcttgt tgacccaggc gaaggtgtcc
      901 gcgacggctt gtccgtatgg cgttccgacg tccgaggtgg ggccggaata atccgcaaga
      961 tggccgatcg cgatctgggc ctgcgccgtc gctgatgcgc cgccaatcag caaggtcagt
     1021 gaaacggtgc taagtaagga tttcatggtc atattatgtt tcctccgttt aatttgttgg
     1081 gtcgattgta gccggtcccg ttccaacctc aatgcgaaaa cgggtagagt ttccagtagg
     1141 tcttgatttg gcgccagcgg tgcgcgagcc cgtccggctc gaacatcaaa aacgcgatga
     1201 tgatcagccc gatcgcgatc tcgcgcagga aggtgatgtt gttattgagc gacagcgcct
     1261 tgtcgataac gctgcctttc aggctggcgc tgatccactc catcgattcc ggcagcagca
     1321 ccacgaaggc ggtgcccatc agcgtgccca tgatcgaccc ggtgccgccg ataatgacca
     1381 tcgccagaaa aatgatcgag cgttcgatgc cgaaaccttc attggatacg acgagctgat
     1441 aatgcgcgta cagtgcgccc gcgatgccgg caaagaaagc cgcaagcccg aatgacaggg
     1501 tgcggtattt tgtcaggttg atgcccatga tctccgcgga gagatagtgg tcgcggatcg
     1561 ccaccagcgc gcggccatcg cgggtgcgca tcaaattggt caccaagagg tagctgacca
     1621 ggacataggc cagcaccacg tagaaatatt ggcggtcgcc gcggaaggtg tagccgaaaa
     1681 tcgagaacgg gttggcgctg gccggcaccg aaccgccgga gaaccagtcg gcgcgcgaga
     1741 agaagtcgag caggatgtat tgcgcggcca gggtcgcgat gacgagataa agccccttta
     1801 gccgcgccgc cggcacgccg aagatcaggc cgaccaacgc ggtgaccacg cccgccagcg
     1861 gaatggcaaa aaacaccggg atcggcagcg tgttggagat ataggccgag gtgaatgccc
     1921 cgaacaaaaa gaatgcggca tggccgatcg aaatctgccc ggtaaagccg accagaatgt
     1981 tcaggccaag ggctgcgatc g
//
LOCUS       DASWGM010000613         3462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_598170,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000613 DASWGM010000000
VERSION     DASWGM010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598170"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VGA15_17365"
     CDS             <1..444
                     /locus_tag="VGA15_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9589503.1"
                     /translation="DFPFPREYRGVYLDRRRESGFQLYNSLGIPRGDKAGYAKQALEN
                     FNFFGAPHVAIVHTDEALGVYGAIDCGGYVTSFMLAAQALGVATIPQAALAFHSELVR
                     KHFGLGDDRRVVCGISFGFPDRGHKANSYRTTRASVADTVTFFEE"
     gene            complement(625..1410)
                     /locus_tag="VGA15_17370"
     CDS             complement(625..1410)
                     /locus_tag="VGA15_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9589504.1"
                     /translation="MPEVAATASAPLLDIDGARATIRLNRPKHLNRLQADDLDALLKL
                     FDRIEADPAVRVMVLTGTGRAFSAGYDLNSVAERATSAIEEKSAGSEFEVVVNRLEDL
                     GVPSICRINGGVYGGATDLALACDFRIGVDTCEMFMPAARLGLHYYKSGIRRYVSRLG
                     ADNARKLFLTAQKIGAAEMLRIGYLTAMTPVEALDEEVANLAAILAGNAPVAMRGMKR
                     TINEIARGELDEDAADQRHRDSMRGAEIKEGIKAFAEKRPPRF"
     gene            complement(1422..2534)
                     /locus_tag="VGA15_17375"
     CDS             complement(1422..2534)
                     /locus_tag="VGA15_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HEX9589505.1"
                     /translation="MSKPLAFPGLAEFAAATAGRNMTKAAYVAVAAGVAMMVLLTVDP
                     AYEAAHHWVDALLWGCLAFFVFEWAVRLRHAVLTRRAWAYALSGRGLVDAVGALAVPL
                     ALVLGANPKTAWLLGILWMFKVVPGIPGLRQLRRVLVQESGPLLSVLVIFLMVLFLAS
                     VSVYFLERDVQPASFGSVPAALWWAIATLTTVGYGDVVPITPLGRLVAAVVMICGLGV
                     FGLWTGILATGFAAETRRDNFLKTWESVSKVPFFAALGPAAIADVTHMLRTMDLPART
                     MIIRKGQTGDCMYFIAAGEVEVDLPGKKVTLGEGAFFGEMALLGNNLRSANITTKRVS
                     RLLVLDLVDFRMLMARHPDLAETIDAEARRRELENK"
     gene            2834..>3462
                     /locus_tag="VGA15_17380"
     CDS             2834..>3462
                     /locus_tag="VGA15_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9589506.1"
                     /translation="MDTSHFAEHAGSAASLFASVFVVATLSMRTMIPLRVFGILTNVV
                     LIASSIPTHNYPTGALQTVLLVLNSYRLHQMLQLVREVKKSVNSDLSMEWLKPFMAER
                     QCAAGEVPFYKDEKAEEMLYIVSGRFKLVESGIELPVGAIVGELGMLSPSNMRTQTLE
                     CIEAGLILSVSYTKVEELYVQNPAFGFYFLRLASARLFQNIATLEQRLA"
BASE COUNT          668 a         1112 c         1062 g          620 t
ORIGIN      
        1 gactttccgt tcccgcgcga atatcgcggt gtctatctcg atcgccgccg cgagagcggc
       61 tttcaacttt acaactcgct gggcattccc agaggtgaca aagcgggcta tgcgaaacag
      121 gcgcttgaaa acttcaattt cttcggcgcg ccgcatgtcg ccatcgtcca caccgacgaa
      181 gcgctcggtg tctacggcgc gatcgattgc ggcggctatg tcacgagctt catgctggcg
      241 gcgcaggcgc taggcgtggc cacgatcccg caagcggcgc tggcgtttca ttccgaactg
      301 gtgcgcaagc actttgggct cggcgatgac cggcgggtgg tgtgcggcat ttcattcggc
      361 ttccccgacc gcggccacaa ggccaacagc taccgcacca cgcgggcgag tgttgcggat
      421 accgtgacgt tctttgagga gtagtgatcg cgcgtcccgg acgcggtgca gcgctcttcg
      481 cgctgctccg cagagccggg acccattgat gtaatgcatg gaccccgatc agcagcgcac
      541 cacgccgcaa gccgtagcgc gtcgaagacg cgcgtgaacg cgctgatggc gctgcgcagc
      601 atccggggaa cgcatccgcg tcaatcaaaa cctcggcgga cgcttttccg cgaacgcctt
      661 gatgccttcc ttgatctcgg cgccgcgcat gctgtcgcga tgccgctggt cggcggcgtc
      721 ttcgtcgagc tcgccgcggg cgatttcgtt gatggtgcgc ttcatgccgc gcatcgcgac
      781 cggcgcattg ccggcaagaa tcgcggcaag gtttgcgact tcctcatcca gcgcctcgac
      841 cggcgtcatc gcggtgaggt aaccgatccg caacatctcc gcggcgccga tcttctgcgc
      901 ggtcaggaac agttttctgg cgttgtcggc gccgagccgc gacacatagc gcctgatgcc
      961 gctcttgtaa taatgcagcc caagccgcgc cgccggcatg aacatctcgc aggtgtcgac
     1021 gccgatgcgg aaatcgcagg ccagcgccag atcggtcgcg ccgccataga cgccgccatt
     1081 gatccggcag atcgagggaa cgccgagatc ctcgagccgg ttgaccacga cctcgaactc
     1141 cgatccggcg ctcttctctt cgatggcgct ggtggcgcgc tcggcgaccg agttgagatc
     1201 gtagccggcg ctgaaggcgc ggccggtgcc ggtgagcacc attaccctga cggccggatc
     1261 ggcttcgatc cggtcgaaca gttttagcag cgcatcgaga tcatcggcct gcagccggtt
     1321 caaatgtttt ggtcggttga ggcggatggt ggcgcgcgcg ccatcgatat cgagcaacgg
     1381 cgcgctcgcg gtcgcggcga cttcgggcat gcgtctctcc attacttgtt ttcaagctcg
     1441 cgccgcctgg cttcggcgtc gatggtctcg gccagatcgg gatgccgcgc catcagcatg
     1501 cggaagtcca cgagatcgag caccaggagc ctcgacaccc gcttggtggt gatattggcg
     1561 gagcgtaaat tgttgccgag cagtgccatc tcgccgaaga acgcgccttc gccgagcgtc
     1621 accttcttgc ccggcaaatc gacctcgacc tcaccagcag cgatgaaata catgcaatcg
     1681 ccggtctggc ccttgcggat gatcatggtg cgcgccggca gatccatggt gcgcagcatg
     1741 tgcgtgacat cggcgatcgc ggcgggaccc agcgcggcaa agaacggcac cttgctcacc
     1801 gactcccagg ttttcagaaa attgtcgcgg cgggtttcgg cggcaaagcc ggtggcgaga
     1861 ataccggtcc agagcccgaa cacgccgagg ccgcagatca tcaccacagc cgccaccagc
     1921 cggccgagtg gcgtgatcgg caccacgtcg ccatatccca ccgtggtcaa ggtcgcgatc
     1981 gcccaccaca aggccgccgg cacgctgccg aagctggccg gctggacgtc gcgttccagg
     2041 aaatacacgg acaccgaggc gagaaacagc accatcagaa agatcaccag cacgctcaac
     2101 agcggcccgg attcctgcac cagcacccgg cgcaattgcc gtagtcccgg aattcccggg
     2161 accaccttga acatccacaa tatgccgagc agccacgccg ttttgggatt cgcgccgagg
     2221 acgagggcga gcggtaccgc cagggcgccg acggcgtcca ccagcccgcg gccggataaa
     2281 gcataagccc atgcacgccg cgtcagaacg gcgtggcgaa gccgcactgc ccattcgaac
     2341 acaaagaagg caaggcagcc ccacagcagc gcgtcgaccc agtgatgcgc cgcttcatag
     2401 gcgggatcga ccgtgagcag aaccatcatg gccacgccgg ccgcgaccgc gacataggcc
     2461 gccttggtca tgttgcggcc ggccgtcgcg gctgcgaatt ctgccagacc agggaaagca
     2521 agcggcttgg acattgcgag gcggagcccg tcggtaaaaa gcccggatac ggcgtgctgc
     2581 gcgcggccgg ttcgacaaag tgcccgggcg gtggcagccc gtcaacgacg gcccgaaaac
     2641 agcgttatcg ccggggacgg ggaaagtcgg ccctcctcaa atcgcagtta tcggccggga
     2701 tgagccataa tccctgacaa aacgtcgctt ccccccctta tatcaaagac tccccccgca
     2761 cattatgatg gggggcgcgc gctggaacag aatcgcgaaa aaccctcatt ccctgaaaat
     2821 tggttggcct ccaatggata cgtctcattt cgcagaacat gcaggatccg cagctagcct
     2881 gttcgcgtca gtcttcgtgg tcgcgaccct ttcgatgcgg acgatgatcc cgctgcgggt
     2941 tttcggcatt ctcaccaacg tcgtcctgat cgcgtcctcg attcctactc acaattatcc
     3001 gaccggggcg ttgcagacgg tgctgctggt gctcaattcg tatcggctgc accagatgct
     3061 gcaattggtg cgcgaggtga aaaagtcggt caacagcgac ctgtcaatgg aatggctgaa
     3121 gccgttcatg gccgagcgcc aatgcgcggc cggcgaggtg ccgttctaca aagacgagaa
     3181 ggccgaggaa atgctctaca tcgtcagcgg tcgattcaag ctggtggaat caggtatcga
     3241 actgccggtt ggtgccatcg tcggcgaact cggcatgctg tcgccgtcca acatgcgaac
     3301 ccagacgctg gaatgcatcg aagccggtct catcctcagc gtcagctaca ccaaggtcga
     3361 ggaactttac gtgcagaatc cggccttcgg cttttacttt cttcgtctcg ccagcgcccg
     3421 gctgtttcag aatatcgcaa cgctggagca gcggctggcg ca
//
LOCUS       DASWGM010000614         3830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1863968,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000614 DASWGM010000000
VERSION     DASWGM010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1863968"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VGA15_17385"
     CDS             <1..492
                     /locus_tag="VGA15_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEX9589507.1"
                     /translation="PIQLRDMSPERLLVIADVLAKSVVLADDEREVAIVFEIIEPFAK
                     ELADHGRTRRDRKGVLQLIGNALLVQHRVSGRVAIGEKPDALWDRPDLERLYARLEDE
                     YELKERVDTLSRKLAVIAETANTLADIIDTRRSLRLELIVVVLIAFEIVITFYQIYAA
                     RGH"
     gene            582..1367
                     /locus_tag="VGA15_17390"
     CDS             582..1367
                     /locus_tag="VGA15_17390"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9589508.1"
                     /translation="MEDIAARRAAESLLAEHKANVRFRPLGPPDGPATISDAYDIQER
                     YVALLRSEHGDAAGYKVGLTSAPMQAFCGIDHPIAGVVLARRVYRSGATVRRADFGRL
                     GLEFEIAVRIKSDVPVTGVPCTAEMIAPHIGGVCAAIELVDDRNADYANLDVLSLLAD
                     NSWNGGIVLSEFATKWPHLESVLGRATKNGVAIGEGHGRDILGHPFNSAAWLATQLAS
                     RGVQLKAGQVVMTGSVMKTIFPSEDASYRFDLEEIGSVEVQVT"
     gene            complement(1491..1703)
                     /locus_tag="VGA15_17395"
                     /pseudo
     CDS             complement(1491..1703)
                     /locus_tag="VGA15_17395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
     gene            complement(2179..2367)
                     /locus_tag="VGA15_17400"
                     /pseudo
     CDS             complement(2179..2367)
                     /locus_tag="VGA15_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 family protein"
     gene            complement(2346..3623)
                     /locus_tag="VGA15_17405"
     CDS             complement(2346..3623)
                     /locus_tag="VGA15_17405"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9589509.1"
                     /translation="MKFAPSLSRARGPADGNPRRFNNKRQDMIEDRVLIAGAGPVGLV
                     AAAQLVRKGIPVTVLEAGNDLGAESRASTFHPPTLDMLDDLGVARQLIDEGLKAPKLQ
                     YRSKRDGVIAQFDFAEIADRTRHPYRVQCEQFKLTRILLASLRNDSNFTIEFGARLEN
                     VRQDDDGVQVSVRVNDRDQIRTGRWLIGADGARSEVRRSLGVEFEGFTWPERFLVLST
                     PFDFDAVIPDLVSVNYVADPECWQFLLRIPGMWRVMFPIPQDVGDEAALTAEFGQSLL
                     ARVVPGVRGFDILHTTLYRVHQRVAKQFRVGRAFLAGDAAHINNPLGGMGMNGGIHDA
                     VNLTSRLAAVYGGQVADDDLDRYDAQRRLVTLESVQTQTIQNKRDLEAKDEQSQLEFR
                     DRLRAIAADPDARKIYLERVSMIASLRRAGELG"
BASE COUNT          776 a         1177 c         1132 g          745 t
ORIGIN      
        1 ccgattcagc tgcgggatat gtcgcccgaa agacttcttg tcattgccga tgtgctggcg
       61 aagagcgtcg tgcttgccga tgacgaacgc gaagttgcaa tcgtgttcga gatcatcgag
      121 ccctttgcca aagagctggc ggatcatgga cgaacccgaa gggaccgaaa gggggttttg
      181 cagctgatcg gaaatgccct gctggtccag cacagagtat ccggtcgcgt cgccatcgga
      241 gaaaagccgg atgcgctctg ggaccgccca gacctggagc ggctttacgc ccggcttgaa
      301 gacgaatacg aactcaagga gcgcgtcgac acgcttagcc ggaagctcgc cgtgatcgcg
      361 gaaacggcca atacgcttgc cgacatcatc gacacccgcc gatcgctgcg cctcgaactg
      421 attgtcgtgg tgctgatagc gttcgaaatc gtcatcacat tctatcaaat ctatgccgcc
      481 aggggccact gatgccggag tcagcgggga cggtccgaat cgcgccactg tttgacagca
      541 gcccctggcg cgttaagatt tgcgaaaaag ggagaggacc catggaggat atcgcggccc
      601 ggcgcgcagc cgaatcgctg ctcgctgaac acaaggccaa cgtacggttc aggccgcttg
      661 gtccgccgga cgggcccgct acgatctcag atgcctacga tatccaggaa agatacgtag
      721 cgctcctccg gagcgaacac ggcgacgccg cgggatataa ggtcgggcta acgtcggccc
      781 cgatgcaggc gttttgcggg attgatcatc ccatcgccgg cgtcgtactc gccaggcggg
      841 tgtaccgatc aggagcgacc gtgcggcgcg cggatttcgg gcgactgggc ctggaattcg
      901 aaatcgccgt ccgcatcaaa tcggacgtcc cggtcacggg cgtgccctgt accgccgaaa
      961 tgatcgcgcc tcatatcggc ggcgtttgcg cggccatcga gctcgtcgac gatcgaaacg
     1021 cggactatgc caacctcgat gttctctcgc ttcttgcgga caattcctgg aacggcggca
     1081 ttgtcctgtc agaatttgca accaaatggc cacatctaga aagcgtcctc ggacgtgcga
     1141 cgaaaaacgg tgtcgcgatc ggcgaagggc acggccgcga tatcctcggg catcccttca
     1201 attcagccgc gtggcttgcc acgcaactgg cttcgcgggg cgtgcagctg aaagcggggc
     1261 aagtcgtcat gaccgggagc gtcatgaaaa ccatctttcc gtcggaagac gcaagttatc
     1321 gttttgatct tgaggaaatc ggttctgtcg aggtgcaggt cacgtaagca agtattggaa
     1381 gaaaggagcg ctagctagag accagcgcga tcgaggtcga caacaacgcc gtccggatta
     1441 tcggataaag acgttccgca ataaaaacgt tccgcgagcc cgtgggcgtc ctacaggtta
     1501 cgcaggacgt tgcgccgaaa cgaatcgata tgggcgcgca tcgcgttctc ggcagcctcg
     1561 acgtcgcggt ggcagatcgc ctggaaaatc ctgtggtgct gatcgttgac gctgccctga
     1621 tgatccggcg cggccagcga aatgaaccac agccgcaacg agcgctcgtg aagcttcaac
     1681 agaatttccg caagcaccgt gttgtgcgcg gcgcgcgatc aatcggtgga actcccggtc
     1741 cagcaacatc atggtctcga ccttgccggc cgcaaccgcg ctgcctgcct tcgccaacac
     1801 gtcgcgtatc gccatgatct cggcggaact ggccctctcc gccgcgaggc gaaccgcgaa
     1861 tgtttcgttg agaagccgga tctcgatgat ttcgatgact tcgtcgagat tgataggccg
     1921 aaccacgacg cccttgcgcg gctgaacgtc gaccagacct tctgtattca ggcgatcgaa
     1981 ggcctggtga acgggcgtcc tcccgatctt gagaaggccg gcgaccgcgc tttcgctcag
     2041 ttcctgcccc ggcttgaatg cacaggtgat gatgcgatgc ttgatcgcca cataggcctg
     2101 gtccttcaag gactccgcca gaaccggctc cgccgcacgg tccttgcgca gccgcgtgat
     2161 ttggttcatc ggccgccctc aacccgcgat cggcagagac aggacatcat aaccgtggct
     2221 gatcgtccgg ccgatgacat cgtcgacaat ctcgaaatcg aaacgcgggg ccggccggat
     2281 tccaccgatg gcgctgaagt gcgtggacat gatttttccc gtgaattttc gttgcatcgg
     2341 ttgtttcagc cgagttcgcc ggcgcgccgc aagctcgcta tcattgagac gcgttcgagg
     2401 tagatcttgc gcgcatcggg atctgccgcg atcgccctga ggcgatcgcg aaactcgagc
     2461 tggctttgct cgtcctttgc ctcgagatcg cgcttgttct gaatggtctg ggtctgaacg
     2521 ctttccagcg tcaccagccg gcgctgggcg tcgtaacgat cgaggtcatc gtccgcaacc
     2581 tgtccgccgt agaccgcagc cagccgcgac gtcaggttca cggcatcgtg gatgccgccg
     2641 ttcatcccca tgccgccgag cggattgttg atgtgcgcgg cgtcgccggc caggaaggcg
     2701 cgccctaccc tgaactgctt ggcaacgcgc tggtgcaccc gatagagcgt cgtatgcaga
     2761 atatcgaagc ccctgacgcc gggcacgacg cgcgccagca gcgactgccc gaattcagct
     2821 gttaacgccg cctcgtcgcc gacatcctgc ggaatcggaa acatcacccg ccacatgccg
     2881 ggaatacgca gcaaaaactg ccagcactcc ggatcggcga cataattgac cgaaaccaga
     2941 tcgggaataa ccgcatcgaa atcgaacggc gtgctcaaca ccagaaagcg ttccggccag
     3001 gtgaagcctt cgaattcgac gccgagcgaa cggcgaactt cactcctggc gccgtcggcg
     3061 ccgatcagcc agcggccggt ccggatctgg tccctgtcgt tgacccgtac cgacacctgc
     3121 acgccatcgt catcctgccg aacgttctcg agccgggctc cgaactctat tgtgaaattg
     3181 ctgtcgttgc gcagactggc cagcaatatc cgcgtcagtt tgaactgctc gcattgcacc
     3241 cgatatggat gtctggtccg gtccgcgatc tcggcgaaat cgaactgcgc aatcacccca
     3301 tcccttttcg accgatattg cagcttcggg gccttcaggc cttcatcgat gagctgacgt
     3361 gccacgccga gatcgtcgag catgtcaagc gtcggcggat ggaatgtcga tgccctcgat
     3421 tctgcgccga gatcgttgcc ggcctcgagc accgtgacgg gaattccctt gcgcaccagt
     3481 tgagccgcgg caacgagacc gacgggtcct gcccccgcaa tcaacacgcg atcttcgatc
     3541 atatcttgtc tcttgttatt gaatcttcgg gggttgccat cagccgggcc tcgcgcccgc
     3601 gacaatgagg gagcgaactt cattcttgat tcgatcggat actgcgcgat cctcccacgc
     3661 cagcgatgga acggtgattt ccgccatcaa accgcgccgg cgcgccaaac accaggattc
     3721 ggtttgcgag aaagattgct tcgtcgatgc tgtgggtaac gatcagaaac gtggtctgtt
     3781 gcgactgcgc cagcgtcttg aaatcctgcc gcagccgcga acaacccgca
//
LOCUS       DASWGM010000615         6935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2570403,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000615 DASWGM010000000
VERSION     DASWGM010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2570403"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..423)
                     /locus_tag="VGA15_17410"
     CDS             complement(43..423)
                     /locus_tag="VGA15_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589510.1"
                     /translation="MKKSPFRLTPYQVQFLLVVGFVTVGYALYLRYMAIELSTVALAC
                     DAGLQTMLCKTRILATYLFKNSVFGIVALVIATLHLIRPSIVLLTGGLIVAGFGIVLY
                     NIGLSGLALGLLILGFARPAPATA"
     gene            complement(625..1878)
                     /locus_tag="VGA15_17415"
     CDS             complement(625..1878)
                     /locus_tag="VGA15_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589511.1"
                     /translation="MAQDSDHLAGFDTENTGGFLSGLLAEEDHIDRRALWRLGSWGVA
                     TIGAIIVAVLASQSSIGLRREQMTSADLALQSQQIQSVAKESQSEARRLASAIETLNS
                     DRDRLYARVTVLEQGLDTVTGAIARQNSAPARPQAATTAPATPEPPQPASQNPAPAPV
                     VSPVAAAPAKPAEKPPADTAPAKDSSPAAVSPVAQAPQAQAPQNSPKNSPAVTPATPL
                     MASKSMLAPPDAPAGKLIPSETPMSAITASPMPEVVASAQAGDVAERDPPKVAVQRTE
                     FGVDVGSANSLGGLRALWRGLLRSKSNAPLAALRPIIVVKESNSGLGMQLRLVAGPLA
                     DAAAAAKICAVLLESERTCETTVFDGQRLSMKAEEVPASVVKPSASRRRGSGKRAVQE
                     EPPKKPETSTTSTLSSMFSRVTRAQ"
     gene            2056..3414
                     /gene="glmU"
                     /locus_tag="VGA15_17420"
     CDS             2056..3414
                     /gene="glmU"
                     /locus_tag="VGA15_17420"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX9589512.1"
                     /translation="MTGRTSLTIVLAAGEGTRMQSSIPKVMHPVAGQSLLAHVLGAAP
                     KGAGAALAVVIGPDHGSVAEETRRLRPDATTFVQRERLGTAHAVLAARDAIARGADDL
                     LVVFGDTPLISAETLARLRAPLKNGAALAVLGFRAADPTGYGRLVVEGDQLVAIREQA
                     EAAPAERAITLCNAGVMAFDGHRALKIIEKIGNANSKGEYYLPDAVGIVRQQGLEAVV
                     IETSEDEVRGINTKAQLAEAEQVMQARLRKAALDAGVTMIAPETVYLAADTKFGGDVT
                     IEPFVVIGPGVTIADGAVIHCFSHLVQSSIGMNASVGPYARLRPGTSLGEGARVGNFV
                     EIKAATLEAGVKVNHLSYIGDTHIGTGANIGAGTITCNYDGFGKHQTTIGSGAFVGSN
                     TSLVAPVKIGSGAYIGSGSVITRDVPDDALAVERGQQTMREGWAKRFREMKSRGKTPK
                     GG"
     gene            complement(3547..3927)
                     /locus_tag="VGA15_17425"
     CDS             complement(3547..3927)
                     /locus_tag="VGA15_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589513.1"
                     /translation="MDLVIQVKRAAPYIEIHDEIEFIFDEQMTEKRHIKYAWEGLKTT
                     RPDIANFLGGEPKWVNDKDFLPIQAADKLAWWTRRQKEELIAGKPQTPSPWTVGRIVP
                     QIMLELDDAALTNILGHHNARQPT"
     gene            complement(3949..4260)
                     /locus_tag="VGA15_17430"
     CDS             complement(3949..4260)
                     /locus_tag="VGA15_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589514.1"
                     /translation="MKLQAFVDDSRTGDANQPGGVFTLAGYVASESEWSAFSSEWQAE
                     LNNDPKIDAFKMSHFSNRFGPWNDRLQLFRNIIERHARGAMTITVELDVYKRFAGSIP
                     I"
     gene            4858..6684
                     /gene="glmS"
                     /locus_tag="VGA15_17435"
     CDS             4858..6684
                     /gene="glmS"
                     /locus_tag="VGA15_17435"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX9589515.1"
                     /translation="MCGIVGILGRGPVAEQLVDSLKRLEYRGYDSAGVATLEGDHLER
                     RRAEGKLKNLEARLKAEPLAGHTGIGHTRWATHGRPTENNAHPHATDRVAVVHNGIIE
                     NFRELREALQSKGTVFKTETDTEIVLHLVDDFLKQGFKPAEAVKATLSQLRGAFALGF
                     IFAGDDNLMIGARNGPPLAVGYGEGEMYLGSDAIALGPFTDTVAYLDDGDWVVLTRNS
                     AVIYDKNNAAVRRDAIKHTGATSLVDKANYRHFMAKEIHEQPEVVGHTLARYVDMATE
                     RVALPVKLPFDFRDIQRISIVACGTASYAGCVAKYWFERLSRLPVEVDVASEFRYREA
                     PLRKGDLAIFISQSGETADTLAALRYAKGEGVHTLSVVNVASSTIARESETVLPTLAG
                     PEIGVASTKAFTCQLMVLAAIAVAAGKARGELSDADEARLVHGLVEIPRLMAAALATE
                     PQIEKLARDIAKSKDVLYLGRGTSYPLALEGALKLKEISYIHAEGYAAGELKHGPIAL
                     IDENMPVVVIAPFDRVFEKTVSNMQEVAARGGNIILMTDAKGAAEATIEQLVTIVLPD
                     MAATFTPMVYAVPVQLLAYHTAVIMGTDVDQPRNLAKSVTVE"
BASE COUNT         1360 a         2118 c         2126 g         1331 t
ORIGIN      
        1 ggcaatcgct gtggacctgc gcggtgtatt ttctgttcgc ggttacgctg tggcgggcgc
       61 gggccgtgca aatcccaaaa taagcaggcc gagcgctaaa cccgacaggc cgatattgta
      121 gagcacgatc ccgaatccgg cgacgatcag cccgcccgtg agcaggacga tcgacggccg
      181 gatcaggtga agcgtcgcga taacaagcgc gacgatgccg aacaccgaat tcttgaacag
      241 ataggtcgcg aggatgcggg tcttgcacag catggtctgc aggccggcat cgcaggccag
      301 cgccaccgtc gagagctcga tcgccatata acgcagatag agcgcgtaac cgacggtgac
      361 gaagccgacg accaacagaa attgcacctg atagggcgtc agccggaacg gagatttttt
      421 cacgagaaca ttttcttccg gctaagggga atccggctag ccgcaggaaa atgcgtctga
      481 ccaatcaggg cgaatatggt cgggaatgcg cggcgggaca acccggcgcg ggcaggttct
      541 tcgaaggggc cgagaaaaag cactggaaaa cggccccaag gcatggcgat aaggctttac
      601 gataaggcat tgcgatgatg agctttactg ggctcgcgtg accctgctga acatcgacga
      661 cagcgtggag gttgtggagg tttccggctt tttcggcggt tcctcctgca ccgcgcgctt
      721 tccgctgccc cgcctgcgcg atgcagaagg cttaacgacg gacgcgggca cctcttcggc
      781 cttcatggaa agacgttggc catcgaacac cgtggtctcg caggtccgct cgctttcgag
      841 caggacggca cagatcttcg ccgccgccgc ggcatcggcg agcggtccgg caaccagccg
      901 caactgcatg ccgagcccgc tgttgctttc cttcaccacg atgatcgggc gcaacgccgc
      961 cagcggcgca ttcgacttcg acctgagaag gccgcgccac agcgcgcgca ggccgccgag
     1021 cgaattggcg ctgcccacat ccaccccgaa ttcggttcgc tggaccgcga ccttgggggg
     1081 atcccgttcc gctacgtccc cagcttgcgc cgacgcaacc acctccggca tcggcgatgc
     1141 ggtgatcgcg ctcatcggcg tctcggatgg gatcaatttc ccggccggag catccggcgg
     1201 agccagcatt gatttcgagg ccatcagcgg tgttgccggc gtcaccgcag gagaattttt
     1261 gggggaattt tgcggggcct gtgcttgggg ggcctgggcg acgggcgaga ccgcggccgg
     1321 acttgaatcc ttcgcagggg ctgtgtccgc cggcggcttt tcagctggct tcgctggcgc
     1381 agccgccacc ggagacacaa ccggagcagg agccggattt tgcgacgccg gctgcggcgg
     1441 ctccggtgtc gcaggcgcgg tcgtggcggc ttgcggccgt gccggggccg aattttgccg
     1501 cgcaatcgcg ccggtcaccg tatccagtcc ctgttccaga acggtgacgc gcgcatacaa
     1561 ccggtcgcgg tcgctgttga gcgtttcgat cgcggaggcc agtcgccgcg cctcgctctg
     1621 gctttccttg gcgacggact ggatttgctg cgactggagc gccagatcgg ctgacgtcat
     1681 ctgttcccgg cgcaatccga tcgatgactg gctggcgagc accgcgacga tgatggcgcc
     1741 gatcgtggca acaccccacg atccgagccg ccacagcgca cggcggtcga tgtgatcctc
     1801 ctcggccaac agaccgctca ggaatccgcc ggtgttttcg gtgtcgaagc cggccagatg
     1861 gtcggaatcc tgtgccatag gacgttctcg gccctcccga ggccttagcc gaatcagagg
     1921 caaacattaa caggaattgc accgtggtct tgaattcgcc gcgttttccc gcgtgacgaa
     1981 agcggtttcc atggcagaag aaaacgtttt atgacgacgg cggcgcaact tcgtcgcgcc
     2041 tgacagggaa tcgacatgac cggcagaacc agcctcacga tcgtactcgc ggccggcgag
     2101 ggcacgcgca tgcagtcctc gataccgaag gtgatgcatc ccgtcgccgg ccaatcgctg
     2161 ctggcgcatg tgttgggggc cgccccaaag ggcgcaggcg ctgcgctcgc cgttgtcatt
     2221 ggacccgacc acggttctgt cgcggaagag acaagacgtc tgcgtccgga tgcaaccacg
     2281 tttgttcagc gcgagcgtct cggcaccgcg catgcggtgc tggcggcccg cgacgccatc
     2341 gcccgcggcg ccgacgatct gctggtcgtg ttcggcgata cgccgctgat ttcggccgag
     2401 accttggcgc ggttgcgggc gccgttgaaa aacggcgcgg cgctggccgt gctcggcttt
     2461 cgcgccgccg atcccaccgg ctatggcagg ctcgtggtcg agggcgatca gctggtggcg
     2521 atccgcgaac aggccgaggc ggcgcctgcg gagcgcgcga tcacgctttg caatgccggc
     2581 gtgatggcgt ttgatggcca cagggcgctg aaaatcatcg aaaaaatcgg taacgccaac
     2641 agcaagggcg agtattatct ccccgacgcc gtcggcatcg ttcgtcaaca gggactagag
     2701 gccgtcgtga tcgaaaccag cgaggatgaa gtgcgtggca tcaacaccaa ggcccagctc
     2761 gccgaagccg agcaggtgat gcaggcgcgc ctgcgcaagg cggcactcga cgccggcgtg
     2821 accatgatcg cgcccgagac ggtttacctc gccgccgata ccaaattcgg cggcgacgtc
     2881 acgatcgagc cgtttgtggt gatcggtccc ggcgtgacga ttgccgacgg cgcggtgatc
     2941 cattgctttt cgcacctcgt gcagtcatcg atcggcatga atgcgtcggt cggcccctat
     3001 gcgcggttgc ggccgggaac ctcgctgggc gagggcgcca gggtcggcaa tttcgtcgag
     3061 atcaaagccg cgacgctgga agccggcgtc aaggtcaatc atctcagcta tatcggcgac
     3121 acccacatcg gcacaggcgc caacatcggc gccggcacca tcacctgcaa ctatgacggc
     3181 ttcggcaagc accagaccac gatcgggtcg ggcgcattcg tcggctcgaa tacgtcgctg
     3241 gtcgcgccgg tcaagatcgg cagcggcgcc tatatcggat cggggtcggt gatcacccgc
     3301 gacgtgcccg acgatgcgct tgcggtggaa cgcggccagc agaccatgcg cgagggctgg
     3361 gccaagcgat ttcgcgagat gaaatcgcgc ggcaagacgc cgaaaggcgg ctaactaacg
     3421 gcgggcgctg tcaagctttc ggcttaacgc tgtcgcaatc cgcaataggt cgaatgcgac
     3481 tttcctcgat aggcgaggaa cattggtcga acaccccgat tttaggacgc atatcggtag
     3541 tatgcgttag gtcggttggc gcgcgttatg atgcccgagg atattcgtca aagctgcatc
     3601 atcgagttcc aacatgatct gcggaacgat gcggcctacg gtccaaggcg acggcgtctg
     3661 cggtttgcct gcgatcaatt cttctttctg tcgtcgtgtc caccatgcca acttgtcagc
     3721 ggcctggatc ggcaagaagt ccttgtcgtt cacccatttc ggctcacctc caagaaaatt
     3781 cgcgatatca ggacgtgtgg ttttcaaacc ctcccacgca tatttgatgt ggcgtttctc
     3841 tgtcatctgc tcatcgaaga tgaattcgat ctcgtcatga atttcgatat agggagcggc
     3901 tcgcttcacc tgaattacta aatccatgaa tgcaatgtag taagggtctc atatcgggat
     3961 cgatccagcg aatcttttat agacgtccaa ctccaccgta attgtcattg ccccgcgcgc
     4021 atggcgttcg atgatgttgc gaaaaagctg aagtcgatca ttccatgggc caaatcgatt
     4081 tgaaaaatgc gacatcttaa aagcatcaat tttcgggtcg ttattaagct ctgcctgcca
     4141 ttcgctagag aacgcgctcc attccgattc cgaagccaca tagccggcca gggtaaatac
     4201 accacccggc tgattagcat cgccggtcct gctgtcgtct acgaatgctt gcagcttcat
     4261 tagttctcag gtcctaaacc agctccccga gcatcaaaat agccaatgac ctgtccgcat
     4321 cggcgcccaa cgcggcaggc acacccgtac tcgatgcacg agtcgttttc tgttgcctcc
     4381 cttagcgcac tgtcaacatg ccaccgctca cttcatattc gccatgacgc ctgccgttcg
     4441 tgatggaaat ccattctttc ggctttgaag atcgttttgc cacagtagac ctctctgggc
     4501 gctattggcg aagcagcttt tggacttgtg cggcagcgtc gggcatcctg tgattgccag
     4561 gtccaaaatc gaggggaggg caggatcggc gagacgactg atctcgggac cggaacccga
     4621 atcgcttggt taccgtacat tcaatttaag atggaagacg agcaatttag ctcagcatgg
     4681 cgccacgctt cccgaccctg tcgattcctg ggacataggc ggccagcgct gttaagcttg
     4741 cggtttaacc ctgtcgcaat cggcaataat tgttgagtat ctggctaccc gcgattctcg
     4801 ctaagagatg ggcgcgctga tgttgaaaat tttcgacgat tggagatatt gatcggcatg
     4861 tgcggcattg tcggcattct cggacgcggt ccggtcgcgg agcagttggt ggattcgctc
     4921 aagcgcctcg aataccgcgg ttacgattcc gcaggggtcg ccaccctgga gggcgaccat
     4981 ctcgagcggc gccgcgccga aggcaaactg aagaatctcg aagcgcgatt gaaagccgaa
     5041 ccgctcgcag ggcataccgg catcggacat acccgctggg ctacccacgg caggccgacc
     5101 gagaacaatg cgcatccgca tgcgactgat cgcgtcgccg tggtacataa cggcatcatc
     5161 gagaatttcc gcgagttgcg cgaagctttg cagagcaagg gcacggtttt caagaccgag
     5221 accgacaccg aaatcgtcct gcatctggtc gatgattttc tcaagcaggg cttcaagccg
     5281 gccgaggcgg tcaaggcgac attgtcgcag cttcgcggcg ccttcgcact cgggttcatc
     5341 ttcgccggcg acgacaatct gatgatcggc gcccgcaatg ggccgccgct cgccgtcggc
     5401 tatggcgaag gcgaaatgta tctcggctcc gatgccatcg cgctcggtcc gttcaccgac
     5461 accgtcgctt atctcgatga cggcgactgg gtcgtcctga cgcgcaacag tgcggtcatc
     5521 tacgacaaga acaacgcggc cgttcgtcgc gatgcgatca agcataccgg cgcgacgtcg
     5581 ctggtcgaca aggcgaatta ccgccacttc atggccaagg aaatccacga acagccggag
     5641 gtggtcggcc acacgctggc gcgctatgtc gatatggcga ccgagcgcgt cgcgctgccc
     5701 gtcaaactgc cgttcgactt cagggacatc cagcgcatct cgatcgttgc ctgcggcacc
     5761 gcgagctatg cgggttgcgt cgccaaatac tggttcgaac gcttgagccg cttgcccgtc
     5821 gaagtcgatg tcgcctcgga attccgttat cgcgaggcgc cgctgcgcaa gggcgatctg
     5881 gcgatcttca tctcgcagtc cggcgagacc gccgataccc tcgccgcgct gcgctacgcg
     5941 aagggggaag gcgtgcacac gctctcggtg gtcaacgtcg cgagttcgac gatcgcgcgc
     6001 gagagcgaga ccgtgctgcc gacgctcgcc ggtccggaaa tcggcgtcgc ctcgaccaag
     6061 gccttcacct gccagctgat ggtgctggcc gcgatcgcgg tggccgccgg caaggcgcgc
     6121 ggtgaattgt ccgatgccga tgaagccagg ctcgtgcacg gcctcgtgga aatcccgcgg
     6181 ctgatggccg cggcactcgc caccgaaccg cagatcgaaa aactggcgcg cgacatcgcc
     6241 aaatccaagg acgtgctcta tctcggccgc ggcaccagct atccgctggc gctggaaggc
     6301 gcgctgaagc tgaaggaaat ttcctacatt catgccgagg gctatgccgc cggtgaactc
     6361 aagcacggac cgatcgcgct gatcgacgag aacatgccgg tggtggtgat cgcgccgttc
     6421 gaccgggtgt tcgagaaaac cgtctccaac atgcaggaag tggcggcccg cggcggcaac
     6481 atcattctga tgaccgacgc caagggggcc gcggaagcga ccattgaaca gctggtcacg
     6541 atcgtgctgc ccgacatggc cgcgaccttc acgccgatgg tttatgccgt tcccgtgcag
     6601 ttattggcct atcatacggc cgtcatcatg ggcaccgacg tcgaccagcc gcgcaatctc
     6661 gcgaaatcgg tgaccgtgga ataggcagat agtggtagcc atgccagctg ataccggtcg
     6721 cttgaagtct tcaaaaatct gctagaagac ttagctgtca ggatgcgaca gccatttcga
     6781 ccacctggaa cccgcatgaa ccgcgacgac ctgcctccgt ctgtgccccc ggccgaagcc
     6841 ccgccagaag tccacagcgg cttgatggcc cgattccgga actattttct gactggcctg
     6901 atcgtcgccg gcccgatcgc catcaccttc tatct
//
LOCUS       DASWGM010000616         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1429457,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000616 DASWGM010000000
VERSION     DASWGM010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429457"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VGA15_17440"
     CDS             complement(<1..801)
                     /locus_tag="VGA15_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX9589516.1"
                     /translation="MCGRPHRLFGLAVPGGRVVAVVAARQTGSPAFGWRTTVRRQRGR
                     LAAASGPVLALVLGVILADALGMAATSPASAQSFTYNPQPPRPKPPPAANNGQMLVQA
                     VEVDYDYNNSRVSAVGNVQMFYNGTSVEADKVIYDQKTKRLHAEGNIRMTDAEGKVTY
                     ANIMDLSDDYRDGFVDSLRVDTADATRMAASRADRSSGNYTVFENGVYTACAPCKDDP
                     KKPPLWQVKGARIIHDQTDKMLYFENAQLEFFGVPMAYLPYFSTPDPTV"
     gene            complement(747..1844)
                     /gene="lptG"
                     /locus_tag="VGA15_17445"
     CDS             complement(747..1844)
                     /gene="lptG"
                     /locus_tag="VGA15_17445"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX9589517.1"
                     /translation="MSMMTNTLGRYFAGRFVVSALGVFASIFVLLVLVDYIEMVRKTS
                     GLVSASAITVAETSLFRVPQLLEKMMPFCILIGAMTCYLALSRRLELVVARAAGVSAW
                     QFIAPALASSIVLGILATTAYNPMSANLRELSKRMEAELFGSTPGGGIQDASGFWINQ
                     VNSDGQAIINAVRSEQQGVRLTGLTVFRFDTDFKFKERIEAREATLEEGRWLFKSARR
                     YSLDGPPVDQDSFFLTTSLTPAQVRNSFSTPETVSFWQLPGYIRSSESSGFATAGYRL
                     QYHKLIAQPFLLAAMVMLAAAVSLRFFRFGGVQKMVLSGVGAGFLLYVLSKVTEDLSK
                     AELMHPIASAWLPVCVGGLTGFLALLYQEDG"
     gene            complement(1841..3010)
                     /gene="lptF"
                     /locus_tag="VGA15_17450"
     CDS             complement(1841..3010)
                     /gene="lptF"
                     /locus_tag="VGA15_17450"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX9589518.1"
                     /translation="MGSIDRYIFRTTLASFALVLVSLTGVIWITQALRGIDLMTSQGQ
                     TIITFLGVTSLVIPALVLIISPIALMIAISHTLNKLATDSEIIVMNAAGFSPLRLFRP
                     FLYATVVVAIMVAFIASYLAPDGLRRLKQWDAEITADVLANILQPGRFAQLDQNLTIR
                     IRERQPGGLLVGIFVDDRRDPKERVSIIADHGTVLKNDSGSFLVLEDGNLERFEEGKR
                     DPALVAFGRYAFDMSKFSNQGHDVTLGIRERYLWELLSPPSDDPVYVQLPGQFRAELH
                     DRFMAPIYPFAFAALTFAFLGAPRTTRQSRNFSITGSILAVFGLRMAGFACSVMAAKS
                     PLAPIVQYLMLVAAVGASLWIIVGGIVIEPPAALMEAINKSNARLLRLFGRPATA"
BASE COUNT          660 a          927 c          953 g          543 t
ORIGIN      
        1 cacggtcggg tcgggcgttg aaaaatacgg cagatacgcc atcggaacgc cgaagaactc
       61 caactgcgcg ttctcgaagt acagcatctt gtcggtctgg tcgtgaatga tgcgcgcgcc
      121 cttgacctgc cacaatggcg gcttcttcgg atcgtcctta caaggcgcgc aggccgtgta
      181 gacaccattc tcgaacacgg tgtagttgcc gctggagcgg tccgcgcgcg acgcggccat
      241 tcgcgtggcg tcggcggtgt cgacgcgcag cgaatcgacg aaaccgtcgc ggtagtcgtc
      301 gctcaaatcc ataatattgg cgtaggtgac tttgccttcc gcatcggtca tgcggatgtt
      361 gccttcggca tgaagccgct tggtcttctg atcgtaaatg accttgtcgg cctcgacgct
      421 ggtgccgttg tagaacatct gcacgttgcc gaccgccgac acgcgcgagt tgttgtagtc
      481 gtaatccacc tccaccgcct ggacgagcat ctgcccgttg ttggcggccg gcggcggttt
      541 cggccgggga ggctgcggat tgtaggtaaa gctctgcgcc gaggccgggg atgtcgccgc
      601 catgccgagc gcgtcggcaa gaatgacccc caaaaccaga gcaagaacgg gtccgctggc
      661 ggcggccaga cgaccacgct gacggcgcac ggtggtgcgc cacccgaacg cgggcgaccc
      721 tgtctggcgg gcggcgacga cggccactac ccgtcctcct ggtacagcaa ggccaaaaag
      781 ccggtgaggc cgcccacaca tacaggcagc cacgccgaag cgatcggatg catcaattca
      841 gccttactca aatcttcagt tactttcgaa agaacataga gcagaaagcc tgcacccacg
      901 ccacttaaaa ccatcttttg cacgccgcca aaacggaaga accgcaggct gacggctgct
      961 gccaacatga ccattgcggc cagcaaaaat ggctgtgcga taagcttatg gtactgcaag
     1021 cggtagcctg cggtcgcaaa tcccgagctt tccgacgagc ggatatagcc ggggagttgc
     1081 caaaaagaca cggtctccgg cgtggagaaa ctgttgcgga cctgggccgg cgtcaggctg
     1141 gtggtgagga agaaactgtc ctggtcgacg ggtggtccgt cgagggagta ccggcgggcg
     1201 gatttgaaca gccagcgtcc ctcctcaagc gtcgcttcgc gcgcctcgat tcgctccttg
     1261 aacttgaagt cggtatcgaa ccggaacacc gtaagcccgg tcagccgcac gccctgctgt
     1321 tcgctgcgga cggcattgat gatggcctgg ccatcgctgt tcacctgatt gatccagaag
     1381 ccggatgcgt cctgtatgcc gccgccggga gtggagccga acagttccgc ctccattcgc
     1441 ttggaaagct cgcgcaaatt ggcggacatc gggttgtaag cggtcgttgc caggattccg
     1501 agcacgatcg agcttgccag cgccggtgcg atgaactgcc acgccgaaac gccggcggcg
     1561 cgtgcgacca cgagttccag tcgccgcgaa agcgccagat agcaggtcat cgctccgatc
     1621 aggatgcaaa acggcatcat tttttcgagc agttgcggca cacggaacag cgacgtctcc
     1681 gcgaccgtga tggcggatgc cgaaaccagc cccgacgtct tcctgaccat ttcgatgtaa
     1741 tcgaccagca ccagaagaac gaagatactg gcgaatacgc cgagcgccga gaccacgaag
     1801 cggccggcaa aatatcgccc aagcgtgttg gtcatcatgc tcatgctgtg gctggccgcc
     1861 cgaaaagccg caagagacgc gcattcgatt tgttgatcgc ttccatcaat gccgcgggcg
     1921 gttcgatgac gatgcccccg acgatgatcc agaggcttgc gccgaccgcc gctaccagca
     1981 tcagatattg aacgatcggt gcgagtggcg atttcgccgc catcacggag caggcgaagc
     2041 ccgccatccg cagcccgaac acggcgagga tcgaaccggt gatcgaaaag ttgcggctct
     2101 gacgcgtggt gcgcggtgcg ccgaggaacg caaaggtcag ggcggcaaat gcgaacggat
     2161 agattggcgc catgaaacgg tcatgcagct cggcgcggaa ctgtcccggc aactgcacgt
     2221 agaccggatc gtcggatggc ggcgagagca attcccagag atagcgttcg cgaatgccca
     2281 gcgtgacgtc gtggccctga ttcgagaact tcgacatgtc gaatgcatag cggccgaatg
     2341 ccaccagcgc gggatcgcgc ttgccttcct cgaagcgttc gagatttccg tcctcgagaa
     2401 ccagaaacga accgctgtcg ttcttcagaa cggtgccatg gtcggcaatg atgctgaccc
     2461 gttccttggg atcgcggcgg tcatcgacga agatgccgac cagcaggcca cccggctgcc
     2521 gctcccgaat gcggatggtc aggttctggt caagctgggc aaagcggccg ggctggagga
     2581 tgttggccag cacatcggcg gtgatttcgg catcccattg cttcaggcgc cgcaggccgt
     2641 cgggggcgag ataggaggca ataaaggcga ccatgatcgc caccacgacg gtggcataga
     2701 gaaaaggacg aaacagccgc agcggcgaga agccggccgc attcatcaca atgatctctg
     2761 agtcggtcgc aagcttgttc agggtatgcg agatcgcgat catcagcgcg atcggcgaga
     2821 tgatcagcac cagcgccgga atcaccaggc tggtgacgcc gagaaaggtg atgatggttt
     2881 gaccctggct ggtcatcagg tcgatgccgc gcaacgcctg cgtaatccaa atcactccag
     2941 tcaggctgac cagaaccagc gcaaacgacg caagcgtcgt gcggaagata tacctgtcga
     3001 ttgaccccat cgttaccgca cgatccccgc cccccgaact ggaacggcag ctcgcgacca
     3061 atcccgaaat cgggatcccc ttt
//
LOCUS       DASWGM010000617         8852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1291187,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000617 DASWGM010000000
VERSION     DASWGM010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291187"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VGA15_17455"
     CDS             <1..717
                     /locus_tag="VGA15_17455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX9589519.1"
                     /translation="QGVDSVLARLQGSLDQIEGRQIAALSSVEESMESRVRRMRGVFT
                     DLGLDMAQLEAATPRAGVGGPFVPVRLPANAGAFERQLYRINTTRAQVERLNRTLALV
                     PYRKPVVGEVEFTSGFGVRSDPFLGRPAMHTGLDFRAAMGDPVRATANGKVVSSGWAG
                     GYGRMIEIDHGNGLSTRYGHLSEIGVKVGDQIRIGQVIGAVGSTGRSTGPHLHYETRI
                     DGDAVDPQKFLRAGVRLSAG"
     gene            complement(735..1973)
                     /locus_tag="VGA15_17460"
     CDS             complement(735..1973)
                     /locus_tag="VGA15_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589520.1"
                     /translation="MPIWMRSLVALAGFAAAAALTATAASAEKKYDPGASDTEIKIGN
                     IMPYSGPASSYGVIGKTEAAYFNMINAEGGINGRKINFISYDDAYSPPKAIEQARKLV
                     ESDEVLLIFQSLGTPSNSAIMKYMNAKKVPQLFVASGGTKFGDPKNFPWTMGFQPNYQ
                     SEGRIYAKYILEHFPNGKIAVFWQNDDAGKDQYKGLRDGLGDKINMIIADKSYEVSDP
                     SIDSQIVALHDSGADIFFSWAAPKGSAQAIRKVGELGWKPKFFLANTATSIASVLKPA
                     GVEYAKGIISTAYIKDPTDPTWKDDPGVKKWREFMDKYYPDGDKANSNNAYAYVESQA
                     MIQVLQQCGDDLTRENVMKQAANLKNFTSDMLLPGVVINTSPDDYFPIEQMQLMRFNG
                     EAWELFGDVITGEIGHDASH"
     gene            2099..4288
                     /locus_tag="VGA15_17465"
     CDS             2099..4288
                     /locus_tag="VGA15_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9589521.1"
                     /translation="MAVSDIHKAGRLNPKLRIGPRHPRLGRAGFHAARWLGARLLGAA
                     GFHQLGSDFADARMARVREKLCRGETVYLAGIGPPGTHNSGVALVEVTQANGPRLIVN
                     NEEERFSGNKHTTEYPVASIDAMVATLRGIGRDIGDIDAWLTSWDYPGLAGTLVRSVL
                     EELPQSVRLLRTTEAAGFDGRRLDQMTRTPKILGRQFGLSERVPLICMPHHDNHAWFS
                     FAASPFACDHDPVAIAVLDGTGDLGSISLYVAEAKVMRRLYCNDSVFDSLGAFYSVIS
                     STQGGWTWLSSEGRYMGAAAWGDMNRASNPYYARLKDVLHFGGNGEVRLNRAVANWYC
                     DPFDHPYKPALIAILGPPLRPDQLWNPDAVLRVEDINHRPDTRDRLDKAAATQLVFED
                     AMIHVVDHLLRLTGANRLVLSGGVALNAVGNMRLLEHFDEAWFAKSQHRRTRLHLWVP
                     PTPGDPGVTIGAAWLFAHLAGAPRGAAMTHAFYCGSSPSHAEIATALEAGDIASTRIG
                     DISTPQGRDALADLMAFAVAKNGIIALYQGAAETGPRALGHRSIFANPCDPQTRERLN
                     ERVKYREAIRPLAPMATLQAAQEYFDLHQGASDGNYNAYNYMVLTAHSKPPARTKIPA
                     VIHADGTGRIQIVRAEDDPLTHAYLKALGRHIGVEISVNTSFNVAGPIAQTPSQAIDT
                     LRRSKGLDAVLLVASDGAAYAAWHGGNRDSGRFTGWLSEWQTASAGR"
     gene            4374..4799
                     /locus_tag="VGA15_17470"
     CDS             4374..4799
                     /locus_tag="VGA15_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589522.1"
                     /translation="MEGDVVKLSRTATLTGCAGLVLGSFLLAPMAHAAQQGCSVEKLQ
                     GRYVFTGQGTNLHYGAFDFDGAGKFLGKQTSLRDKTSIQREALGGTYHLDADCTGTMT
                     LEGQPGGTAHWDVFVTSDGKKGRMIRTDSGTTGVRTFEQ"
     gene            complement(4866..5823)
                     /locus_tag="VGA15_17475"
                     /pseudo
     CDS             complement(4866..5823)
                     /locus_tag="VGA15_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609762.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            complement(5956..6360)
                     /locus_tag="VGA15_17480"
     CDS             complement(5956..6360)
                     /locus_tag="VGA15_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589523.1"
                     /translation="MKRIFLILVIGFALSLGACSRDPGPKGEPGAQGPAGPQGAQGIQ
                     GVAGAQGQAGAQGPQGPQGTPGEKGEKGDKGDKGDKGEAAAVNLRSVQADGAASCEAN
                     ETLVSVFCPSGGAPDGAKCGTSPTVGLCLRKP"
     gene            complement(6479..7366)
                     /locus_tag="VGA15_17485"
     CDS             complement(6479..7366)
                     /locus_tag="VGA15_17485"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9589524.1"
                     /translation="MTKVAFIGLGRMGHGMAGRYLDGGFTVAVWNRSKAKAEDLIARG
                     ARWAASPADAADGADAIVTMVADDEASRAVWLGKDGAVRTAKPGTLAIECSTVSYGHA
                     LELARELRESGLVYIDSPVTGLPDAAASGKLTLLVGAEPADLERARPYLTPLSTTIRH
                     FGAVGNGTVYKLINNLMGAIQIAGIAEGLAIAEQAGLDMKLVLEAIETGVAGSPQVIR
                     HSKRMVARNFAGANFTAALRHKDAAYAVALAESLLSAVPVMGRAAAQAYDRAKAYAPD
                     DDEGKMIEIVSRAKPGKAK"
     gene            complement(7370..8501)
                     /gene="prfB"
                     /locus_tag="VGA15_17490"
     CDS             complement(join(7370..8431,8433..8501))
                     /gene="prfB"
                     /locus_tag="VGA15_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502756.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX9589525.1"
                     /translation="MRAEVERLVEEIKQSVGLLRRHLDVDASTARLAELNKLAEDPNL
                     WNDPQKAQKLMQERTSLEDSLSGIGKVERELEDNIGMIELGEAENDEGVVAEAETALK
                     ALKKEVARRELEALLGGEADRFDSYLEVHAGAGGTESQDWAAMLLRMYTRWAEKHGFK
                     IEYLEETQGEEAGIKSATIQISGNNAYGWLKTEAGVHRLVRISPFDSNARRHTSFSSV
                     AIFPVVDNSIKIDIAESDVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGVAVVCQAGR
                     SQHKNKAQAWDMLRARLYEIELKKREEQAAADQAAKTDIGWGHQIRSYVLQPYQMVKD
                     LRTGVQTSDTSGVLDGDLDEFMAATLAQRAFGAPPPSIEDVD"
     gene            complement(8643..>8852)
                     /locus_tag="VGA15_17495"
     CDS             complement(8643..>8852)
                     /locus_tag="VGA15_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589526.1"
                     /translation="IKLITVDQMSGMRAGPGSGRTVLEAFKPGTAPPDNYSVIGVADA
                     DGRTMLQPSPDADRSLFRPGTGGLY"
BASE COUNT         1610 a         2810 c         2720 g         1712 t
ORIGIN      
        1 cagggcgtcg atagcgttct ggcccgcctg cagggttcgc tcgatcagat cgaaggccgt
       61 cagatagcgg cccttagctc ggtcgaagag agcatggaat cgcgcgttcg ccggatgcgc
      121 ggcgtgttca ccgatctcgg cctcgatatg gcgcaattgg aagccgcgac gccacgcgcg
      181 ggtgtcggcg gccccttcgt tcccgtcaga cttcccgcca acgccggcgc ttttgagcgc
      241 cagctttacc ggatcaacac cacccgcgcc caggtcgagc gtctcaaccg cacccttgcg
      301 ctggtgccat atcgcaagcc ggtggtcggc gaagtcgaat tcaccagcgg attcggggtc
      361 cgcagcgatc cgttcctcgg ccgcccggcc atgcacaccg gtctcgattt ccgcgccgcc
      421 atgggcgacc cggtgcgggc gaccgcgaac ggaaaggtgg tctcgtcggg atgggctggc
      481 ggctatggcc gcatgattga aatcgatcat ggcaacggcc tgtcgacccg ctacggccat
      541 ctatcggaga tcggcgtcaa ggttggcgat cagatcagaa tcggacaggt gatcggcgcg
      601 gtgggctcaa ccggcagatc gaccggtccg catttgcact acgaaaccag gatcgacggc
      661 gacgccgtcg acccccagaa attcctgcgc gccggggtgc ggctcagcgc gggctgatcc
      721 ggcgcaaccg cgcctcaatg gctggcgtcg tggccgatct caccggtgat gacgtcgccg
      781 aacaattccc acgcctcgcc attgaaccgc atcagctgca tctgttcgat cgggaagtag
      841 tcgtccggtg aggtgttgat cacgaccccc ggcagcagca tgtcgcttgt gaagttcttc
      901 aggttggcgg cctgcttcat gacgttctcg cgcgtcagat cgtcgccgca ctgctgcaag
      961 acctgaatca tcgcctgcga ttccacatag gcgtaggcgt tgttggagtt ggccttgtcg
     1021 ccgtcggggt aatacttgtc catgaactcc cgccacttct tcacgcccgg atcgtccttc
     1081 caggtcgggt cggtcggatc cttgatgtag gcggtcgaga tgatgccctt ggcatattca
     1141 acgcccgccg gcttcaagac cgaggcgatg ctggtcgcgg tgttggcgag gaagaatttc
     1201 ggcttccagc cgagttcgcc gactttgcga atcgcctgcg ctgatccttt cggcgccgcc
     1261 caggagaaga agatatcggc gccggaatcg tgtagcgcga cgatctgcga atcgatcgag
     1321 ggatcgctca cctcatagga cttgtcggcg atgatcatgt tgatcttgtc gccgagcccg
     1381 tcgcgcagcc ccttgtattg atccttgccg gcgtcgtcgt tttgccagaa cacggcgatc
     1441 ttgccgttgg ggaaatgctc gaggatgtac ttcgcgtaga tgcggccttc actctggtag
     1501 ttgggctgaa agcccatggt ccaggggaag ttcttgggat cgccgaattt ggtgccgccg
     1561 gaggcgacga agagctgcgg caccttcttg gcgttcatgt atttcatgat cgccgagttg
     1621 gagggcgttc ccagcgactg gaaaatcaac agaacctcgt cgctttccac gagcttgcgc
     1681 gcctgctcga tcgctttcgg cgggctgtag gcgtcgtcgt aggagatgaa gttgatcttg
     1741 cggccgttga tgccgccttc cgcgttgatc atgttgaaat aggcggcttc cgtcttgccg
     1801 atcacgccat aggaggaagc aggaccgcta tagggcatga tgttgccgat cttgatctcg
     1861 gtatcgcttg cccccggatc gtatttcttt tccgctgatg ctgcggtcgc ggtgagcgcg
     1921 gcagcggccg cgaaacccgc caaggcaaca aggctcctca tccaaatcgg catcgttctc
     1981 tccggctatt tattttgtat tttgctcaag tgtcgcaagt catgcgcagg catgcaagcg
     2041 tcggtttatt ccgcccgacg aaacagcatt gttgcgcgcc gccgattatg attgatgcat
     2101 ggcagtatct gacatccaca aggccggccg attgaacccg aaactgcgaa ttggtccgcg
     2161 acatccgagg ctcggcagag ccggatttca cgccgcgcga tggctcggcg cgcgtttgct
     2221 gggggctgcg ggctttcatc agctcggctc ggattttgcc gatgcgcgga tggcgcgcgt
     2281 ccgcgaaaag ctctgccgcg gcgaaaccgt ttatctggcg ggaatcgggc ctcccggcac
     2341 ccacaattca ggtgtcgcgt tggttgaggt gacccaggcg aacggcccgc gcctgatcgt
     2401 caacaacgag gaagagcgct tttccggcaa caagcacacg acagaatatc ctgttgcgtc
     2461 gatcgatgcg atggttgcga cgctgcgcgg aatcggccgc gatatcgggg atatcgacgc
     2521 gtggctgacc agttgggatt atcctggtct ggccggcacg ctggtccgct cggtgctgga
     2581 ggagttgccg caaagcgtga gactgctgcg caccaccgaa gccgccggat ttgacggccg
     2641 ccggctcgat caaatgaccc gcacgccgaa aattctcggc aggcagttcg gcctctcgga
     2701 gagagtgccg ctgatctgca tgccgcacca cgacaatcat gcgtggtttt ctttcgcggc
     2761 gtcgccgttc gcctgtgatc atgaccccgt cgccatcgcc gtgcttgacg gcaccggcga
     2821 cctcggatcg atctcgctgt atgtcgccga agctaaagtc atgcgacggc tctactgcaa
     2881 tgacagcgtg ttcgactcgc tcggcgcctt ctacagcgtg atttcatcga cccagggcgg
     2941 ctggacctgg ctgtcgagcg agggccgcta catgggcgcc gccgcctggg gcgacatgaa
     3001 tcgcgccagc aacccctatt atgcgcggct gaaggacgtg ctgcatttcg gcggcaatgg
     3061 cgaggtgcgc ctcaatcgcg ctgttgccaa ctggtattgc gaccccttcg atcatcccta
     3121 caagcccgcg ctaatcgcaa tcctcgggcc accgttgcgg cccgatcagc tctggaatcc
     3181 ggacgcggtg ttgcgggtcg aggatatcaa ccatcggccc gatacacgcg atcgtctcga
     3241 caaggccgca gcgacccaac tggtgttcga ggatgccatg atccacgtgg tcgatcatct
     3301 cttgcgctta accggcgcga accggctggt actgtcaggc ggcgtggcgt tgaacgcggt
     3361 cggcaacatg cgcctgctcg aacatttcga cgaggcgtgg ttcgcaaaat cccagcaccg
     3421 cagaactcgc ctgcatctgt gggtgccgcc gacgccgggc gaccccggcg tcaccatagg
     3481 cgcggcctgg ctgttcgcgc atctggcggg cgcaccgcgc ggcgccgcga tgacgcatgc
     3541 gttctattgc ggttcatcgc catcgcatgc ggagatcgca accgcgctcg aggccggcga
     3601 catcgcctca acccggatcg gggatatctc gacgccgcag ggccgcgatg cgctggccga
     3661 tctgatggcg ttcgcggtgg cgaaaaacgg tatcatcgca ttgtatcagg gcgcagccga
     3721 aaccgggccg cgcgcgctcg gccaccgctc gatctttgca aatccttgcg atccccagac
     3781 gcgtgaacgc ctcaacgagc gcgtcaaata tcgcgaagcg atccgtccgc tggcgccgat
     3841 ggcgacgctg caagccgcgc aggaatattt cgatctgcat cagggcgcgt cggacggcaa
     3901 ctacaacgcc tacaattaca tggtactgac cgcccattcg aagccgccgg cgcgcaccaa
     3961 aattccggcg gtgattcatg ccgacggcac cgggcggatt cagatcgtgc gggcggaaga
     4021 cgatcccctc acccacgcct atctgaaggc gctcggccgc cacatcggcg tcgagatatc
     4081 cgtcaatacg tcgttcaatg tcgccggccc gatcgcgcag acgccaagcc aagcgatcga
     4141 cacgctgcgc cgatccaagg ggctcgacgc ggtgctgctg gtggcgagcg acggcgcggc
     4201 atatgcggca tggcacgggg gaaatcgcga cagcggccgc tttacgggct ggctctcgga
     4261 atggcagacc gcatcggcag gccggtgatg atctgtgcgc tgcgcggtca gtgccgcatt
     4321 ctttcttggt gttgccgcaa tcttgtcgtg gatgttccat ccgctgcaat gggatggagg
     4381 gcgatgttgt gaagttgagc agaaccgcga cgctgacagg ctgtgcaggc ctggtgttgg
     4441 gaagtttttt gctggccccg atggcccacg cggcacaaca gggatgctcg gtcgaaaagc
     4501 ttcaaggccg ttatgttttc accggccaag gcacaaacct gcattatggc gcgtttgact
     4561 ttgatggcgc gggaaaattt ttggggaagc agacctcgct tcgcgacaag acgtccatcc
     4621 agcgcgaagc cttgggcggc acgtatcatc tggatgcgga ctgcacggga acaatgaccc
     4681 tcgaaggtca accgggcggt acagcacact gggacgtctt tgtgaccagc gacggcaaga
     4741 agggacgcat gatccggacg gattccggta cgacgggcgt ccgcaccttc gaacaatagc
     4801 gtcggctcga agcggcggct ccctcccttg cggtcggggc cgcgctcggg tcgtcggcga
     4861 aggcgctatc gaattgcggg cacgcgtcgg acgaaattga gcacgcgccc gtcgatcagc
     4921 agcagcgcgc tgccgatcac gagggcgccg acgatctcgc gcagcgagat gctttcgccc
     4981 agcacgagat agccgagcag aatagccgtc accggaatga gaagcgtcac cagcatgacg
     5041 ttggtcgagc ccgagcgccg caacacctga aaaaatacga tataggccag cgcagtcgac
     5101 aatccggcga ggcccatcat cgccagccat gtcgttgcgc ccggcatcgg caattgccag
     5161 ggacgttcga ccgcggccgc gatgatcgtc atcatcaggc tggacgccag caattggaac
     5221 gtcgccgtcg caagcggcgg cgagtccgac agtttgcgcc gcgcatagag tgcggctaat
     5281 ccatagctga aggccgcggc gaggcagagc aggatgccga tcccctcccc ggcctgaatg
     5341 cccagatctt gtccatgaag gtcggttcca agaaggtctt gtccatgaag gtcttgccca
     5401 tggagaatga tcacgccgat caaccccgtg acgacgcccg cgacacggcg cgggtgcagt
     5461 ttctcgtcgc cggcggcagc catcacgaga acggtgaaca agggcgtggt tgcgttcagg
     5521 atcgaggcca gtccgctggg gatataggtc tgtccgatga cgatcaaaga aaacattgtt
     5581 gagaagcgcg atcgcgaaaa atggcctcca gccggacaga ccgaccggaa atcgtatgcg
     5641 gtaagcccat aacaccggca gcaggatcat tgcggcgagt gcgacccgca gcagcaccac
     5701 cgtcagcggc ggcaattccc gcagcaccac gccgttgaag aagaacgatc cgccccacag
     5761 caccgagagc agtccaagca gggaccaatc ccgggcatcg atccgattgt cgttttctgt
     5821 cattgcgact catgtctgcg cgaccaggca gacgcctacc tcaatacctc aaaaatcgca
     5881 atgagagcca cccgattccc gcaaacggga tcgcgcgcgt acgagcacga tacgtgcttg
     5941 gctcggcgtc aggtcttaag gcttgcgcaa acaaaggcct acggtcggtg acgtcccgca
     6001 ttttgcgccg tccggcgcgc caccgctcgg acagaacacc gacaccaggg tctcattggc
     6061 ttcgcagctt gccgcgccgt ccgcctgaac ggacctcaga ttgaccgcgg ccgcctcgcc
     6121 tttgtcgccc ttgtcgccct tatctccctt ttcgcctttt tcaccgggtg ttccctgagg
     6181 cccctgcgga ccttgcgcgc cagcctgtcc ctgggcgcca gccaccccct gaatgccttg
     6241 cgcgccctgt ggaccggccg gaccctgtgc gcccggctcc cctttcggtc cgggatccct
     6301 gctgcaggcg ccaagcgaaa gtgcgaatcc aatgacgagt atgagaaaaa ttctcttcat
     6361 gatccccccg caatgattga atgagtcact ggccgcataa agccggcgtc gataccgtcg
     6421 aaccgcgtga gttccgcgac gttagatatc ggagcgtagc gatgcaatag cggcggcgct
     6481 atttcgcctt gccgggcttt gcccgcgaca cgatctcgat catctttcct tcgtcgtcat
     6541 cgggcgcgta ggccttggcc cggtcatagg cctgcgccgc ggcgcgtccc atcacgggaa
     6601 cagcggacaa caggctctcg gccaacgcga ccgcataggc ggcatcctta tgccgcagcg
     6661 cggccgtaaa attcgcacct gcaaaattcc gcgcgaccat ccgcttggaa tgacggatca
     6721 cctgcggact gccggcaacg ccggtctcaa tcgcttccag caccagcttc atgtcgaggc
     6781 cggcctgctc ggcgatcgca aggccctccg cgatgccggc aatctggatc gctcccatca
     6841 gattgttgat cagtttgtag acggtgccgt taccgacggc gccgaaatgc cggatcgtcg
     6901 ttgagagcgg cgtcagatag ggccgcgcgc gctcgagatc agcgggttcg gcgccgacca
     6961 gcagcgtcaa ttttccgctt gccgcggcat ccggcaaacc ggtgaccgga ctgtcgatat
     7021 aaaccagacc agattcacga agctcgcgcg ccagctcaag cgcgtggccg tacgacacgg
     7081 tggaacattc gatcgccagc gtgccgggtt tggccgtcct gacggcccca tccttgccga
     7141 gccagaccgc gcgcgacgcc tcgtcgtcgg cgaccatggt cacaatagcg tcggcgccat
     7201 cggctgcgtc cgcaggcgaa gccgcccatc gcgcgccccg cgcaataaga tcctcggctt
     7261 tcgctttgct gcgattccag accgcgaccg taaagccgcc atcgagatag cggccggcca
     7321 tgccatgccc catgcgcccc aagccgatga aagcaacctt tgtcatttgt cagtccacat
     7381 cctcgatgga aggcggcggc gcgccgaacg cacgctgcgc cagcgtcgcc gccatgaatt
     7441 cgtcgaggtc gccgtcgagc acccccgagg tatccgacgt ctgcacgccg gtacgaagat
     7501 ccttcaccat ctgatagggc tgcaggacat aggagcggat ctggtggccc cagccgatgt
     7561 cggtcttggc ggcctggtcg gcggcggcct gctcttcgcg cttcttcagc tcgatctcgt
     7621 agagccgcgc gcgcaacatg tcccacgcct gcgccttgtt cttgtgctgc gagcgtccgg
     7681 cctgacagac caccgcaacg ccggttggaa tgtgcgtgag tcttaccgcc gactcggtct
     7741 tgttgacgtg ctgtccgccg gcgccgcccg atcgcatggt atcgacacga acgtcggact
     7801 cggcgatgtc gatcttgatg ctgttgtcga ccacgggaaa gatcgcgacg ctggagaacg
     7861 aggtatgccg gcgcgcgttg gaatcgaacg gcgatatccg caccagccgg tgcacgccgg
     7921 cttcggtctt cagccagcca tacgcgttat taccgctgat ctggatggtg gcggatttga
     7981 tgccggcctc ttcgccctgg gtctcttcca ggtattcgat cttaaagccg tgcttttccg
     8041 cccagcgcgt gtacatgcgc agcagcatcg cggcccagtc ctggctctcg gtcccgccgg
     8101 cgccggcgtg aacctcgaga taggagtcga accggtcggc ctcgccgcca agcaatgctt
     8161 cgagctcgcg ccgcgcgact tccttcttga gggctttcag ggcggtctcg gcttccgcca
     8221 ccacgccttc atcgttctcg gcctcgccga gttcgatcat gccgatattg tcctcaagct
     8281 cgcgctcgac cttgccgatg ccactaagcg aatcctccag cgaggtccgc tcctgcatca
     8341 gcttttgcgc tttctgggga tcgttccaga gattggggtc ttcggcgagc ttgttcagct
     8401 cggcgaggcg cgccgtggat gcatcgacgt caaagatgcc tcctcagcag cccgaccgac
     8461 tgcttgatct cttcaacgag gcgttcgact tcagcgcgca tgggttctct gatctgcggc
     8521 acaaagccgc cgttatcaat gcttcgaggg atgtagcggc gcgcgccaca aagcgcaacg
     8581 cttgacgcga accggtttcc accccggatc aagtccgggg cgggctttcg cttgaaaacg
     8641 ccttagtaaa ggccgccggt gcctggacgg aacagactgc gatccgcatc gggggatggc
     8701 tgcagcatcg tccgcccgtc ggcatcggca acgccgatca cggagtaatt atccggcggc
     8761 gcggtgcccg gcttgaaggc ttcgagaacc gtccttccac tgcccggacc ggcccgcatt
     8821 cccgacatct gatcgacggt gatcagcttg at
//
LOCUS       DASWGM010000618         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_330757,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000618 DASWGM010000000
VERSION     DASWGM010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330757"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VGA15_17500"
     CDS             <1..213
                     /locus_tag="VGA15_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX9589527.1"
                     /translation="HKTSMLQDLERGRPMEIDPLVSVVQEMGRLTQIPTPALDAVLAL
                     VAQRANIAGLYEGNVRPAEEKTPVFA"
     gene            339..1334
                     /locus_tag="VGA15_17505"
     CDS             339..1334
                     /locus_tag="VGA15_17505"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX9589528.1"
                     /translation="MIKTRFTELVGVEHPIVQGGMQWVGRAELVAAVANAGALGFITA
                     LTQPTPDDLTREIARCRGLTDKPFGVNLTILPSIKPPPHAEYRQAIIESGIKIVETAG
                     NKPQEHVTEFKRHGVVVVHKCTSVRHALSAERMGVDAISIDGFECAGHPGEDDTPGLI
                     LIPVAADKVKIPLIASGGFGDGRGLVAALALGAEGINMGTRFMCTKESPIHQLIKEKI
                     VANDERETELIFRTMRNTSRVAKNAVSTQVVAMEKEGATFEQVRELVAGARGKMVYAT
                     GDADEGIWSAGQVQGLIHDIPSCAELVSRIMRDAEAIIRSRLEGMMSGSRRQAAE"
     gene            1371..2345
                     /locus_tag="VGA15_17510"
     CDS             1371..2345
                     /locus_tag="VGA15_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9589529.1"
                     /translation="MKAYVYGANGAEITDVAKPAPKGPQVLVRVRACGLNRADLGMTK
                     GHAHGSAGGAGTVLGMEWAGEIADVGPEAKGVKVGDRVMGSGSAAFAEYTLADHGRLF
                     HIPASANMSYEDAATLPVALATMHNAVVTNGALQPGQSVLIQGASSGVGLMALQIAKL
                     KGAKIVIGSSTDAMRRGRLKEFGADLAVDSSDPGWVDQVREATGGEGVDLIVDQVSGK
                     VASQNLAATKIKGRIVNVGRLGGTHADFNFDLHAARRINYIGVTFRTRSIEEIREIFS
                     EVRKDIWGAVESRKLQLPIDKVFAFDEIGKAFEHMEANKHLGKIVVTL"
BASE COUNT          512 a          778 c          797 g          403 t
ORIGIN      
        1 cacaagacct cgatgctgca ggatctcgaa cgcggacgcc cgatggagat cgacccgctg
       61 gtgtctgtgg ttcaggaaat ggggcgattg acacagattc cgacaccggc tctggatgcg
      121 gtgctggcgc tggttgcaca gcgcgcgaat atcgcagggc tgtacgaagg aaatgtccgt
      181 cccgcggagg aaaaaacccc cgtttttgca tgaacgcgtt gccctgacct ctgccttgcc
      241 ccgtggacat ttccggaagg ggacggcatc cttccccgcg aaagttcaca aaatagaacg
      301 ggcgcggccg tccgcgccgg ggaaacagag ggctggcgat gatcaaaacg cgatttaccg
      361 agctcgtggg cgtcgaacac ccgatcgtgc agggtggcat gcaatgggtg ggacgcgcgg
      421 agctggtcgc ggcggtcgcc aatgccggcg cgctcggctt catcaccgcg ctgacgcaac
      481 cgacccccga cgacctcaca cgcgaaatcg cgcgctgccg cggcctgacc gacaagccgt
      541 tcggcgtcaa cctcaccatt cttccgtcga tcaagccgcc gccccacgcg gaatatcgcc
      601 aggccatcat cgagagcggc atcaagatcg tggaaaccgc cggcaacaag ccgcaggaac
      661 acgtcaccga gttcaagagg cacggcgtcg tggtcgtgca caaatgcacc agcgtccgcc
      721 acgcgctgtc ggcggaacgg atgggggtcg acgcgatctc gatcgacggt tttgagtgcg
      781 ccgggcatcc cggcgaggac gatacgccgg gcctgatcct gatcccggtc gccgccgaca
      841 aggtaaaaat tccgctgatc gcctcgggcg gcttcggcga tggccgcggc ctggtcgcag
      901 cactggcgct gggtgccgag ggcatcaaca tgggcacccg tttcatgtgc accaaggaga
      961 gtccgatcca tcaactgatc aaggaaaaga tcgtcgccaa tgacgagcgc gagaccgagc
     1021 tgattttccg caccatgcgc aatacctcgc gcgtcgccaa gaacgcggtc agcacccagg
     1081 tcgtcgccat ggagaaagaa ggcgcgacct tcgagcaggt ccgtgaactc gtcgccgggg
     1141 cccgcggcaa gatggtctac gcgacgggcg acgccgacga aggcatctgg tcggcaggcc
     1201 aggtgcaagg gttgatccat gacatcccca gctgcgccga gctggtgtcg cgcatcatgc
     1261 gtgacgcgga agctatcatt cgcagccggc tcgaaggcat gatgtcgggc agccgccgtc
     1321 aagccgcgga ataggcggcc tcctatcacc cactaacaga gaatgatttc atgaaggcct
     1381 atgtctacgg cgccaacggc gctgaaatca ccgacgtcgc caagcccgct ccgaaaggcc
     1441 cacaggtgct ggttcgcgtc cgcgcctgcg ggctcaaccg cgccgacctc ggcatgacca
     1501 aaggccatgc gcatggcagt gccggcggcg ccggcacggt gctggggatg gaatgggccg
     1561 gcgaaatcgc cgatgtcggg cctgaggcaa agggcgtcaa ggttggcgac agggtgatgg
     1621 gatccggcag tgctgcgttc gcggaatata cgctcgccga tcacggccgg ctgtttcaca
     1681 ttcctgccag tgccaatatg agctacgagg acgcggccac cctgcccgtc gcgctggcca
     1741 ccatgcacaa tgcggtcgtg accaacggcg ccttgcagcc aggccagtcc gtcctgattc
     1801 aaggcgcgag ctccggcgtc ggcctgatgg cgctgcagat cgcaaaactc aagggcgcaa
     1861 aaatcgtgat cggatcctcg accgatgcga tgcgccgcgg gcggttaaag gaattcggcg
     1921 ccgaccttgc agtggattcc agcgatcccg gctgggtcga tcaggtgcgg gaagccaccg
     1981 gcggcgaagg cgtcgacctg atcgtcgatc aggtctcggg caaggtcgcc agccagaatc
     2041 tggccgccac caaaataaaa ggccgcatcg tcaatgtcgg caggctcggc ggcacccatg
     2101 ccgatttcaa tttcgacctg catgcggcgc gccgcatcaa ctatatcggc gtcaccttcc
     2161 gcacccgttc gatcgaggaa atccgcgaga tttttagcga agtccgcaag gatatctggg
     2221 gagcggtgga atcgcgcaag ctgcaattgc cgatcgacaa ggtgttcgca ttcgacgaga
     2281 tcggcaaggc gttcgagcac atggaagcca acaagcatct cggcaagatc gtcgtgacgc
     2341 tgtagcgcgc ctgaacgtat cccggacgcg gtgcaacgct cttgcgttgc tccgcagagc
     2401 cgggacccat tcggcgctca acaagtggac cccggattgc ggtgcaactc gtcgacggcc
     2461 acagccagta ggtccgattg cagctaagga
//
LOCUS       DASWGM010000619         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2012507,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000619 DASWGM010000000
VERSION     DASWGM010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012507"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="queC"
                     /locus_tag="VGA15_17515"
     CDS             complement(<1..691)
                     /gene="queC"
                     /locus_tag="VGA15_17515"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX9589530.1"
                     /translation="MSDQFPSETALVLFSGGQDSATCLAWALQRFARVEMLGFNYGQR
                     HAIELECRGRLLDGMKWLRADWAAKLGESHTLEIPTLATISDTALTRDVAIAMGEDGL
                     PNTFVPGRNLVFLTFAAALAYRRGITHIIGGMCETDYSGYPDCRDETIKALQTALNLG
                     MNKAFELHTPLMWLDKASTWKLAHELGGAGLVDLIREHSHTCYLGERGAQHAWGYGCG
                     ECPACALRARGW"
     gene            822..1637
                     /gene="mazG"
                     /locus_tag="VGA15_17520"
     CDS             822..1637
                     /gene="mazG"
                     /locus_tag="VGA15_17520"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX9589531.1"
                     /translation="MPPSRDISSLIEIMATLRTPGSGCPWDLEQNFATIAPYTIEEAH
                     EVAEAIARGDLDDLRDELGDLLLQVVFHARMAEEQNAFSFGDVVEAISTKMIRRHPHV
                     FADKDGRLTPADVKGAWDRIKAEEKAERAARRPRKETAHKSLLSGVKAGQPALTRAME
                     LQRKASSVGFDWNDPRAVLHKIREEADEIEAALDRGDADELAEETGDLLFALVNLARH
                     VGADPDLALRGTNAKFERRFGYIERALAAQGRSLESASLAEMDALWNAAKGEE"
BASE COUNT          346 a          563 c          575 g          316 t
ORIGIN      
        1 gccatcccct ggcgcgcagc gcgcaggccg gacattcgcc gcaaccatag ccccacgcgt
       61 gttgcgcgcc gcgttcgccg agatagcagg tgtgggaatg ttcgcggatc aaatcgacca
      121 gcccagcccc gccgagttca tgcgccagtt tccaggtgct ggccttgtcg agccacatca
      181 gcggcgtatg caattcgaac gccttgttca tgccgagatt gagtgccgtc tgcaacgcct
      241 tgatggtctc gtcgcggcaa tccggatagc cggaataatc ggtttcgcac attccgccga
      301 taatgtgcgt gatgccgcgc cggtaggcca gcgcagccgc aaacgtcagg aacaccaggt
      361 tgcggccggg cacgaaggtg ttgggcaggc cgtcctcgcc catcgcaatc gcgacgtcgc
      421 gcgtcagcgc ggtgtcggaa atcgttgcca atgtcggaat ctccagggtg tggctctcgc
      481 cgagtttagc cgcccagtcc gcgcgcaacc atttcatccc gtcgagcagg cggccgcggc
      541 attcaagctc gatggcgtgt cgctggccgt aattaaaacc gagcatttcc acgcgcgcaa
      601 agcgctgcag cgcccaggcg agacaggtgg cggaatcctg tccgcctgaa aatagcacca
      661 gcgcggtctc ggatgggaat tgatcgctca tcggctgccc ttagcaccgt caggcgcgcg
      721 cgccaatcgg tggatacagc aaacctatct gctcgttttc gctgggcggg cagcacgtct
      781 tgcggcatac aaagcttggt ttccgcaagc ttcggtgcac catgccccct tcccgcgata
      841 tttcaagcct gatcgagatc atggcgacgc tgcgaacccc cggcagcggt tgcccgtggg
      901 acctcgaaca gaatttcgcg accatcgcgc cctacaccat cgaggaggcc catgaagttg
      961 ccgaggcgat cgcgcgcggc gatctcgacg atctgcgcga cgagctcggc gatctcctgc
     1021 tgcaggtggt gtttcatgcc cgcatggccg aggagcagaa tgcgttttca ttcggcgatg
     1081 tcgtcgaggc gatctccacc aagatgatcc gaaggcatcc gcatgtcttt gccgacaagg
     1141 atgggcgatt gacgccggcc gacgtgaagg gcgcgtggga ccgcatcaag gccgaggaga
     1201 aggccgaacg cgcggcgcgg cggcctcgga aagaaaccgc gcacaaatcg ctgctgtctg
     1261 gcgtcaaggc ggggcagcct gcgttgacgc gcgcgatgga attgcagcgc aaggcgtcaa
     1321 gcgtcggctt cgactggaac gatccgcgcg ccgtactcca caagatccgc gaagaagccg
     1381 acgagatcga ggccgcgctc gaccgcggcg atgccgacga actcgccgag gaaaccggcg
     1441 atctcctgtt cgcgctggtc aatctggcgc ggcatgtcgg cgccgatccc gatttggcgt
     1501 tgcgcggcac caacgccaaa ttcgagcggc gcttcggcta tatcgaacgc gcgctcgcgg
     1561 cacagggccg ctcgctcgaa agcgcctcgc tcgccgagat ggacgcgttg tggaacgcgg
     1621 cgaagggcga agagtagaga gatcagcccc gtcattgcga tcgggatagg ggggaacctc
     1681 acggctcctc ccctcccaca ccaccgtacg tacgggtccg tatacggcgg ttcgatgaat
     1741 taaacagcat ttggtaccgt gacagttgca agcccgggtg aggtgaagaa ggcgtttgat
//
LOCUS       DASWGM010000620         3686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2293339,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000620 DASWGM010000000
VERSION     DASWGM010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293339"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VGA15_17525"
     CDS             <1..424
                     /locus_tag="VGA15_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HEX9589532.1"
                     /translation="ELRGQALLEGGKPAEAIAPLRKAVQLSNNAPLIEMLLGQALVGT
                     DNKAYTEEAISILRAAVARETEAPLGYTQLAMAYGRKGDFAQADLASAQAAFLRGDNK
                     TARELASRAKTRFAVGTPGWVKADDIVAAKPMPGQNSN"
     gene            486..1253
                     /locus_tag="VGA15_17530"
     CDS             486..1253
                     /locus_tag="VGA15_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX9589533.1"
                     /translation="MPSLRLLAPVLFALALCGMQPAASAQSFSDSQRGDIETIVRNYL
                     LAHPEVLEEAMAELSKRQAAADTEKHEASVAKNAETIFNSPRGVTVGNKDGDVTFVEF
                     FDYNCGYCKRAMADMLDLMKSDSKLKVVLKEFPVLGPGSVEAAQVAVAARMQDPAGKK
                     YLDFHQKLLGGRGQADKVHAMAAAKEAGFDMAKLEKDISSPEVRATLEENFKLAEEMG
                     MNGTPSYVIGKQVVVGAVGLDGLKEKIGIARCGKATC"
     gene            1461..2270
                     /locus_tag="VGA15_17535"
     CDS             1461..2270
                     /locus_tag="VGA15_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HEX9589534.1"
                     /translation="MTNRFMMSVAAAALIAGTGFANAQGTGMGREAPSAGSTAQQSAP
                     PSEHAAPSAAPAARDAAEPTKPSSGMKATQSEQPTKPAPGAKATQSEQPPAAAKNQRA
                     QDDIKAAPKGERSAQDTNMKGEKSKSMSSENDNAKGAKDLKAEGREDRTGTKAEGRED
                     RKGNMNAETKGAEGRSQTIGQAGAGAKLSTEQRTKISTVIKEQNIRPVTNVNFAISVG
                     TRVPREVSFHPLPVEIVNIYPDWRGYEMFLVNDQIIVVNPRTLEIVAVLDV"
     gene            2540..3007
                     /gene="aroQ"
                     /locus_tag="VGA15_17540"
     CDS             2540..3007
                     /gene="aroQ"
                     /locus_tag="VGA15_17540"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX9589535.1"
                     /translation="MANTAAETIYVLNGPNLNLLGTREPETYGHATLADVEKLCAETA
                     EQFGLKADCRQSNREGELIDFIHEAHAAKAAGIIINAGGYSHTSIALHDALAAVKIPA
                     VEVHISNIHARESFRHHSFTARAAFATLSGFGIDGYRLAITGLAARIGAKTKT"
     gene            3050..3526
                     /gene="accB"
                     /locus_tag="VGA15_17545"
     CDS             3050..3526
                     /gene="accB"
                     /locus_tag="VGA15_17545"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX9589536.1"
                     /translation="MARQPDDKPAAKFKSEDSALIRELALLLDETSLTEIEIERAGLR
                     VRVARNVSIAATMPANFQPTASASATASAAVDDPAKHPGVVPSPMVGTAYWASEPGAK
                     PFIEVGTKVSVGQTLLIIEAMKTMNQIPSPRAGTVTQILVEDGQPVEFGEPLVIIE"
     gene            3613..>3686
                     /locus_tag="VGA15_17550"
     CDS             3613..>3686
                     /locus_tag="VGA15_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9589537.1"
                     /translation="MFDKILIANRGEIALRVLRACKELG"
BASE COUNT          793 a         1159 c         1127 g          607 t
ORIGIN      
        1 tgaattgcgc ggtcaggcgc tgctggaagg cggcaagccc gcggaggcga tcgcgccctt
       61 gcgcaaggcc gtgcagctct cgaataacgc accgctcatc gagatgttac ttgggcaggc
      121 gctcgtggga accgataata aagcctatac ggaagaagca atctcgattc tgcgtgcggc
      181 cgtggcacga gagaccgaag cgccgctcgg ctatacccag ctcgcgatgg cctatggccg
      241 aaagggagat ttcgcacagg ccgacctggc atcggcgcag gccgcttttc ttcgcggcga
      301 caacaagacc gcgcgcgaac tcgcctcacg cgccaaaacc cgcttcgccg tcggcacacc
      361 cggatgggtc aaggccgacg atatcgtcgc tgcaaagccg atgcccggcc aaaacagcaa
      421 ctagcatgaa ttgagccagg tcaagaaggt tttcggacac cggctttcac caagaggatt
      481 tgcccatgcc ttcgcttcgc ctgctcgccc ccgtcctgtt cgcgctcgcg ctctgcggca
      541 tgcagcctgc cgcgtcggcg cagagtttct ccgacagcca gcgcggggat atcgagacca
      601 tcgtgcggaa ttatctgctg gcgcatcccg aagtgctgga agaggcgatg gccgaactga
      661 gcaagcgcca ggccgccgcc gataccgaga aacatgaagc cagcgtcgcc aaaaacgccg
      721 agacgatctt caactcgccg cgcggtgtca ccgtcggcaa caaggacggc gacgtcacct
      781 tcgtcgagtt cttcgattac aattgcggtt attgcaaacg cgcgatggcc gacatgctcg
      841 atctgatgaa atcagattcg aaactgaagg tcgtgctgaa ggaatttccg gtgctcgggc
      901 ccggctcggt cgaggcggcg caggtcgccg tcgccgcgcg catgcaggac cctgccggca
      961 agaaatatct cgacttccat cagaagctgc tcggcggccg cggccaggcc gacaaggtgc
     1021 acgccatggc ggcggcgaaa gaggccggct tcgacatggc gaaactcgaa aaagatattt
     1081 cgagcccgga ggtccgcgcg acgctggagg aaaacttcaa gctcgccgag gagatgggca
     1141 tgaacggcac gccgagctac gtgatcggca agcaggtggt ggtcggcgcc gtcggcctcg
     1201 atggcctgaa ggaaaagatc ggcatcgccc gttgcggcaa ggcgacgtgc tgagcgtccc
     1261 ctcgatcggt tgaacctgct gcgtacgcga actgaaaggc cggccgaaca gccggccttt
     1321 tatgttgacc gattttcaaa gcgctggcgt tcatcgcgca ttcacaaaac aaacccggtg
     1381 gaaccgcaga taacacggaa catcgcgcat ctcggctcgt tgtcggcgcg ggcaatgcaa
     1441 acgacgtgag gagatgttcc atgactaatc gttttatgat gtcggtggcc gcggcggcgc
     1501 tgatcgccgg caccgggttc gccaacgcgc aggggaccgg aatgggccgc gaggcgccgt
     1561 cggcgggatc cacggcgcag cagagcgcgc caccgtccga gcacgctgcg ccttcggccg
     1621 cgcccgctgc gcgcgatgca gcagaaccga ccaagccgtc gtctggcatg aaggccacgc
     1681 agtctgagca accgaccaag cctgcgcccg gcgccaaggc cacgcagtct gagcaaccgc
     1741 ccgctgccgc caagaaccag cgcgcgcagg acgacatcaa ggccgctccg aagggcgaaa
     1801 gatcggccca ggacaccaac atgaagggcg aaaagtcgaa gagcatgagt tccgagaacg
     1861 acaacgccaa gggggctaag gacctcaagg ccgaaggtcg tgaggaccgc accggcacga
     1921 aggctgaagg ccgagaagac cgcaagggca acatgaacgc cgagacaaag ggcgcggaag
     1981 gcaggtccca gaccatcgga caggccggcg ccggcgcaaa gctctcgacc gaacagcgca
     2041 ccaagatcag caccgtgatc aaggagcaga acatccggcc ggtgaccaac gtcaatttcg
     2101 cgatctcggt cggtacccgt gttccgcgcg aggtgtcgtt ccacccgctt ccggtggaaa
     2161 tcgtcaacat ctacccggac tggcgcggct acgagatgtt cctggtcaac gatcagatca
     2221 tcgtggtgaa tccgcgcacg ctcgagatcg ttgccgtgct cgacgtctaa tactgagcgc
     2281 atagcgagtt tgggggcggg cagcgatgcc cgcccctctt gcgtcaggcg cggcgagcaa
     2341 ggaatcacgc ctgccgcggc gggttggccc caccgcgcca tccgcaacat tggttaacgt
     2401 gtgatttcca caggttatgc gcaccttttt gcgagccgga tgaggcattt gaagcaagcg
     2461 caaagcggcc taaaggcttc cccttggtgc tgttgttacc tataaccgcg ccacctaccg
     2521 ccacattgcc gggatttgga tggccaacac agctgccgag acgatttacg tgctcaacgg
     2581 cccgaacctc aatctgttgg ggacgcgcga gcccgagacc tatggccatg cgacacttgc
     2641 cgatgtcgag aaactttgcg ccgagaccgc ggagcaattc ggcctgaaag ccgattgcag
     2701 gcagtccaat cgcgaaggcg aactgatcga tttcatccac gaggcgcatg ccgcgaaggc
     2761 ggccgggatc atcatcaatg ccgggggcta ttcccacacc tcgatcgccc tgcatgacgc
     2821 tttggcggcg gtgaaaattc cggccgtcga agtgcacatc agcaacattc acgcccgcga
     2881 gagctttcgt catcattcct tcactgccag agccgccttc gcaacacttt ccggtttcgg
     2941 catcgacggc taccggcttg cgatcaccgg ccttgctgcc aggatcggcg ccaagacaaa
     3001 aacctgacgc gttagcccgt caccccacag aaactttcgg atccagatca tggcgcgcca
     3061 gcctgacgac aaaccagccg caaaattcaa gagcgaggat agtgcgctca tccgcgagct
     3121 tgcacttctg cttgacgaga ccagtctcac cgagatcgag atcgagcgcg cgggcctgcg
     3181 ggtgcgggtc gcccgcaacg tcagcatcgc cgcgacgatg ccggcgaatt ttcaacccac
     3241 ggcttccgcg tcggccacag cgtcggccgc cgtcgacgat cccgccaagc acccgggcgt
     3301 ggtgccctcg ccgatggtcg gcaccgccta ctgggcttcc gagcccggcg ccaagccatt
     3361 catcgaagtc ggcaccaagg tgtcggtcgg acagacgctg ctgatcatcg aggcgatgaa
     3421 gaccatgaac cagattccat caccgcgcgc cggcaccgtg acgcagattc ttgtcgagga
     3481 cggccagccg gtcgaattcg gcgaaccgct tgtgattatt gagtaagagg cgaatggcga
     3541 gtagcgaata gcgaatggtt ttcgcgctta ctattcgcca ctcgctattc gctattcgcc
     3601 ccctgggaca acatgttcga caagatcctg atagccaatc gcggcgaaat cgcattgcgg
     3661 gttctgcgcg cgtgcaagga actcgg
//
LOCUS       DASWGM010000621         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1162159,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000621 DASWGM010000000
VERSION     DASWGM010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162159"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..326)
                     /locus_tag="VGA15_17555"
     CDS             complement(183..326)
                     /locus_tag="VGA15_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589538.1"
                     /translation="MFLLVMAVTSMLVTAIHAFLRHAGFGGCCATAGAARDRAVRDAS
                     LTY"
     gene            409..1992
                     /locus_tag="VGA15_17560"
     CDS             409..1992
                     /locus_tag="VGA15_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014287056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589539.1"
                     /translation="MSARAWVGALVAALAVALALPQPGEAKTLKFANDGDVSSMDPYF
                     LNETFLLGFLGNVYEPLVGRGKQLELVPKLAQSWEQLEPNLWRFHLRPNVKFAGGENF
                     TADDVIFSYQRAIGEGSDIKSKLGSVKEVKKVDDLTVDFITTLPNPLLPGDIAFWYIM
                     PKGWAEKNGAIKVAAVSKKEENYATYHTNGTGAFMVTKREPDVRTVAVPNPNWWNKPE
                     HNLTEVEFSVIKSDATRVAALLSGEVDMVYTVPIQDVERVNATAHRKVLQGPEMRTIF
                     LGMDQWRDELIDSDVKGKNPFNDVRVRRAFYQAIDEDAIAKKVMRGAATPSGLMVAPA
                     VSGWDKALNERYPYDTAAAKRLLSDAGYPEGFSVGMNCPNDRYVNDEQICQAVAAMLA
                     RIGVKVNLTAETKSKYFGKILARNTSFYMLGWTPDTYDAQNALFNLMATPNDTGRGKF
                     NLGKYSDPRLDALTTMVVSELDPQKRNAEISEAFKLHKEAFGHIPLHQQALAWGIKDS
                     VQLFQRADNIFNWNWVTMD"
     gene            complement(2019..>2240)
                     /locus_tag="VGA15_17565"
     CDS             complement(2019..>2240)
                     /locus_tag="VGA15_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9589540.1"
                     /translation="PVDLLFADIILPGIDGVQLAKQARLMRPGIKVLFTTGYAQKAIE
                     RGAMRHGKVLFKPVREPELVREVALLLAA"
BASE COUNT          448 a          728 c          692 g          372 t
ORIGIN      
        1 agacgatctt ctcgccgccc ttgcgctggg cgatctcggg aacggtcagg cgccgcgact
       61 gcacctgctt cgacatgatg aacctccgtg gctcagcggc gcgccctccc cgcgggcgat
      121 ctttatttgc agcgctgcca aactagcgca aaccccgatc gccggccaat cttctttggc
      181 cctcagtagg ttaacgacgc atcgcgcacg gcgcgatcgc gggcggcgcc agcggttgcg
      241 cagcaaccgc caaagcccgc gtgacgcagg aaagcgtgga tggccgtgac aagcatgctt
      301 gtcacggcca tgacgaggag gaacatgcga tgccttcttc cgctccgtct tgcgccgcct
      361 ccggcaacaa cctacgcttt tttcggctca cagcagggga ggccgtgcat gtcggcaagg
      421 gcatgggtcg gggcgctcgt cgcggcgctc gcagtggcgc tggcgctgcc gcagccgggc
      481 gaagcgaaga cgctcaaatt cgccaatgac ggcgacgtct cgtcgatgga cccctatttc
      541 ctcaacgaga cctttctcct gggcttcctc ggcaatgtct acgagccgct ggtcgggcgc
      601 ggcaagcagc tcgagctggt gcccaagctg gcgcaatcct gggagcagct cgagccgaat
      661 ctgtggcgct tccacttgcg gcccaatgtc aaattcgccg gcggcgagaa cttcaccgcc
      721 gacgacgtga tcttctccta tcagcgcgcc atcggcgagg gctcggacat caagtcgaaa
      781 ctaggcagcg tcaaggaggt gaagaaggtc gacgacctta ctgtcgactt catcaccacc
      841 ctgccgaatc cgctgctgcc gggcgatatc gccttttggt acatcatgcc caagggctgg
      901 gccgagaaga acggcgccat caaggtcgct gccgtgtcaa agaaggagga gaattacgcc
      961 acatatcaca ccaacggcac cggcgcgttc atggtgacaa agcgcgagcc ggacgtgcgc
     1021 accgtggcgg tgccgaaccc caactggtgg aacaagcccg agcacaattt gaccgaagtc
     1081 gagttcagcg tcatcaagtc ggacgcgacg cgcgtcgccg cgctgctctc gggcgaggtc
     1141 gacatggtct acaccgtgcc gatccaggac gtcgagcgcg tcaacgccac ggcgcaccgc
     1201 aaggtgctgc agggcccgga aatgcgcacg atcttcctcg gcatggacca atggcgcgac
     1261 gagctcatcg acagcgacgt caagggcaag aacccgttca acgatgtgcg tgtgcgccgc
     1321 gccttctacc aggcgatcga cgaggacgcg atcgccaaga aggtgatgcg cggcgcggca
     1381 acgccgtccg ggctgatggt ggcgcccgcg gtcagcggct gggacaaggc gctcaacgag
     1441 cgctatccct acgacaccgc ggcggcgaag cggctgctga gcgatgcggg atatcccgag
     1501 ggcttcagcg tcggcatgaa ctgccccaac gaccgctacg tcaacgacga gcagatctgc
     1561 caggcggtcg ccgccatgct ggcgcgcatc ggcgtcaagg tgaatttgac ggcggagacc
     1621 aagagcaagt atttcggcaa gatcctcgcc cgcaacacca gcttctacat gttgggctgg
     1681 acgccggaca cctacgacgc ccagaacgcg ctattcaatc tgatggcgac cccgaacgac
     1741 accggccgcg gcaaattcaa cctcggcaag tactcggacc cgcgcctcga cgcgctcacc
     1801 acgatggttg tgagcgagct cgacccgcag aagcgcaacg ccgagatcag cgaggctttc
     1861 aagctccaca aggaggcgtt cggccatatc cccttgcacc agcaggcgct ggcctggggg
     1921 atcaaggaca gtgtccagtt gttccagcgc gccgataaca tcttcaactg gaactgggtg
     1981 acgatggatt agcgccgatt ccgaatctct tgcgcgactc aggccgcgag cagcaacgcg
     2041 acttcgcgaa caagttcggg ctctcgcacc ggcttgaaca ggactttgcc atgtcgcatc
     2101 gcgccccgct cgatggcttt ctgtgcatag ccggtcgtga acagaacctt gatgccgggg
     2161 cgcatgagcc tcgcttgctt cgccagttgc acgccgtcga tgcccggcag gatgatatcc
     2221 gcgaacagca aatccaccgg
//
LOCUS       DASWGM010000622        10502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_599055,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000622 DASWGM010000000
VERSION     DASWGM010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599055"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /gene="trmFO"
                     /locus_tag="VGA15_17570"
     CDS             complement(<1..1274)
                     /gene="trmFO"
                     /locus_tag="VGA15_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX9589541.1"
                     /translation="MTGIKTSSESVHIVGAGLAGSEAAWQVANAGVRVILHEMRPGRM
                     TEAHRTDGLAELVCSNSFRSDDAANNAVGLLHAEMRRLGSLIMRAADANQVPAGGALA
                     VDRDGFSAAVTQALHDHPLIEIDRAEIDGLPPADWRNVIVATGPLTSAPLAAAIREIT
                     DENALAFFDAIAPIVHRDSIDMSAAWFQSRYDKVGPGGTGADYINCPMTKAQYDGFVD
                     ALMAGEKTDFKDWETTTPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPHDPTVKAY
                     AIVQLRQDNKLGTLYNMVGFQTKLKHGAQQRVFRTIPGLENAEFARLGGLHRNTFLNS
                     PKLLDAQLRLRAEPRLRFAGQMTGCEGYVESASIGLMAGLYAAADARSTSLAPPPATT
                     ALGSLLSHITGGHIETIDPVARSFQPMNINFGL"
     gene            complement(1427..1579)
                     /locus_tag="VGA15_17575"
     CDS             complement(1427..1579)
                     /locus_tag="VGA15_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX9589542.1"
                     /translation="MLLSLIRMIQAFRDYQRNVSELSQLSDRELADIGLDRSDIPRVA
                     AGNYNG"
     gene            2090..2356
                     /locus_tag="VGA15_17580"
     CDS             2090..2356
                     /locus_tag="VGA15_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589543.1"
                     /translation="MAHVIGQFVLAILESLAELFCFFTGKWLLLLITGGRVVPMPPTT
                     PRSWSLSPFKRLPTGQIGVDSQFVALIALVFWILVGIGLHLLFR"
     gene            2553..2816
                     /locus_tag="VGA15_17585"
     CDS             2553..2816
                     /locus_tag="VGA15_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589544.1"
                     /translation="MIKKLLGAATMAAVAAYTVVPASAAKMAGCSGENMMKTESMIEA
                     MADGEGKIAAQKEIAMAQTAMLDGKMGACGMHLSKAMHVSTSK"
     gene            complement(2891..3655)
                     /locus_tag="VGA15_17590"
     CDS             complement(2891..3655)
                     /locus_tag="VGA15_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX9589545.1"
                     /translation="MPVASAQAAPCGTGSFGAWLDDFKNEAAAKGISPSAIASGLAGV
                     TLDQGVLSRDHSQRVFSQSFEEFSGRMVPPRLTRGASLMKQYGSVLGRIEERYGVPGE
                     VLVAIWGLETDFGVNIGKFPTMRSLATLAYDCRRSDMFKAELMDALRIVERGDLAATE
                     MRGAWAGEIGQTQFMPSSYIKFAVDFDGNGRRDLLRSAPDVLASTANYLASYGWKRGK
                     DWEPGSANFSVIQQWNKSEVYSKTIAYFATQLAHAP"
     gene            complement(4000..4914)
                     /locus_tag="VGA15_17595"
     CDS             complement(4000..4914)
                     /locus_tag="VGA15_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX9589546.1"
                     /translation="MGTPYPGNAGPLAQPAQDSAAGPVIRTIRLSDLHDALRLGWEDF
                     KAVPSHAIILCVIYPILGLVLARTVLGYSVLPLLFPLAAGFALLGPFAALGLYELSRR
                     RERGETASAWDAIDVFRSPSFGAMLGLGTLLFALFVTWVATAQAIYVAAFGYESAAGI
                     PDFVTRVLTTPQGWWLIVVGCGVGLLFAVLALCVSVVSFPLMLDRHAGAGDAMVTSLR
                     AVARNPAPMAAWGLIVAVLLVLGSLPFFLGLAVVIPLLGHATWHLYRKVIAPDLNPQP
                     LPPRRVRERRSAADFPAALFPWRRKDDA"
     gene            5297..5698
                     /locus_tag="VGA15_17600"
     CDS             5297..5698
                     /locus_tag="VGA15_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HEX9589547.1"
                     /translation="MSIFGKIMSAIFGTKADAAPPSGGATAGTATSAGPAAGSSSASA
                     TPAQTVDVAPILDKAVAAKKEKLEWRTSIVDLMKALDIDSSLGARKELAKELGYTGDS
                     NDSASMNIWLHKQVMIKLAANGGKLPADIKH"
     gene            complement(5831..6763)
                     /locus_tag="VGA15_17605"
     CDS             complement(5831..6763)
                     /locus_tag="VGA15_17605"
                     /inference="COORDINATES: protein motif:HMM:NF042732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6683 family protein"
                     /protein_id="HEX9589548.1"
                     /translation="MFAINWHAINRGARSLPKKAHFESDKIKIVWGCTWPPPQRGADL
                     CATRGGVTVRSFKHRAGRGAALLGSALICLCSTAAVAQWNPHFTASMGRGYGSIALSQ
                     SILSGTRRLGAGATVRPNAAPRARAASLTPAQIDAALTYTADPTQSDRIRVAMIDTMG
                     GQNPATREQMTKAFADDVVLKEFDRVMSANGYSSRNVADDMAALLVLCWEIFTGGSAN
                     KLQIEGARQQSRGIFLGTPALRTMTNAERQEMAERIAYQFVISDMANQLALRSGDQAQ
                     RAQVRQSAAAVMRQRVNIDITRLQLTEKGFSKKS"
     gene            complement(6833..7132)
                     /locus_tag="VGA15_17610"
     CDS             complement(6833..7132)
                     /locus_tag="VGA15_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589549.1"
                     /translation="MLLPTVIAAASIRIGEFVRSQRLSELICGTDSVAVLDAPADWSH
                     VSAEFSSFSPKGDRLAVVYAGKIPRGEWRTLIEIWNTDIARKVASRCALVLKAPR"
     gene            complement(7640..7792)
                     /locus_tag="VGA15_17615"
     CDS             complement(7640..7792)
                     /locus_tag="VGA15_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX9589550.1"
                     /translation="MLTAFLFDTIGRYLRYRSQLASVYELDDRTLLDIGLNRGELKAT
                     SWDLVH"
     gene            8008..8799
                     /locus_tag="VGA15_17620"
     CDS             8008..8799
                     /locus_tag="VGA15_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9589551.1"
                     /translation="MVTVDRRTMLATGALALAGAAAQTGGAEAQAGPKPIFLVPVITI
                     PIVGETEVFAVRRIYCIGRNYAAHAIERGSDPTREPPFFFQKPTDAIQNVAIGTVADH
                     PYPLLTKNYHHEVELVAALKSGGTNIAPEQALEHVYGYALGLDMTRRDLQNGMAAEKK
                     PWEIGKSFDHAAVLGPIHPAAKTGHFTKGAISLSVNGTVRQDSNLDKMIWSVAEQISK
                     LSEAFELKAGDIIYSGTPENVGPVVKGDVLLCKLEGLPDMSIRIT"
     gene            complement(9135..9407)
                     /locus_tag="VGA15_17625"
     CDS             complement(9135..9407)
                     /locus_tag="VGA15_17625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9589552.1"
                     /translation="MTDVRDNKAQNRFELDVEGGVAFANYRVTPSTVIITHTETPRAL
                     RGRGIASELVQGALQLIRADGLKVVAGCGFVVDYLGKHPEHADLVG"
     gene            complement(9470..10000)
                     /locus_tag="VGA15_17630"
     CDS             complement(9470..10000)
                     /locus_tag="VGA15_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HEX9589553.1"
                     /translation="MITRRALVLTGATGLAAAVSCPVLAAPPSPNDPVAIINAIYARA
                     AKGKGDGGGAFIIENQAAKAKYLSKALVELWAKADAHTPKGDVGPVDFDPVTNSQEPN
                     VKSFKVVPEKLDADKAVVAVTISGRGAPRPKSVDNTIRYNLVRDAGQWKIDDINGASD
                     GEAWSIRGMLTDSLKN"
     gene            10263..10391
                     /locus_tag="VGA15_17635"
     CDS             10263..10391
                     /locus_tag="VGA15_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589554.1"
                     /translation="MANEKGPNVKKKQKCKNCEGKGLLKKGDKVVKCQRCGGTGLR"
BASE COUNT         2140 a         3136 c         3298 g         1928 t
ORIGIN      
        1 agtccaaagt tgatattcat cggctggaac gagcgcgcaa ccggatcgat ggtctcgata
       61 tggccgccgg tgatgtggct gagcaacgat cccagcgccg tcgtcgccgg cggcggcgcc
      121 agcgacgtcg agcgggcatc ggccgcggcg taaaggccgg ccatcaaacc aatgctggcg
      181 gattccacat aaccctcgca gccggtcatc tgtccggcga agcgcagccg cggttcggcg
      241 cgcagccgca attgcgcatc cagcagtttt ggcgaattca ggaaggtgtt gcgatgcagg
      301 cccccgagcc gggcgaattc ggcgttctcg agcccgggaa tagtgcggaa gacgcgctgc
      361 tgggcgccgt gcttgagctt ggtctggaag ccgaccatgt tgtagagcgt gccaagcttg
      421 ttgtcctggc gcagctgcac gatggcgtag gccttcaccg tcggatcatg tggattggtt
      481 aatccgaccg gcttcatcgg cccgtggcgc agcgtctcgc ggccgcgctc ggccatgacc
      541 tcgatcggca ggcagccgtc gaaatagggc gtagtggttt cccagtcctt gaagtcggtc
      601 ttctcgcccg ccatcagggc gtcgacgaag ccgtcatatt gcgccttggt catcgggcaa
      661 ttgatgtaat cggcgccggt gccgccgggg ccgaccttat cgtagcgcga ctggaaccag
      721 gccgccgaca tgtcgatgga atcccggtgc acgatcgggg cgatcgcatc gaagaatgcc
      781 agcgcattct cgtcggtgat ttcgcggatc gcagccgcca gcggcgcgga ggtcaagggc
      841 ccggtcgcga cgatgacgtt gcgccagtcc gcgggcggca ggccgtcgat ttcggcgcga
      901 tcaatttcaa tcagggggtg atcgtggagg gcctgggtga cggcagcgga gaagccgtcg
      961 cggtcgacgg ccaaggcgcc gccggcggga acctgattgg cgtcggcggc gcgcatgatc
     1021 agcgagccca gccggcgcat ttcggcatgc agcagcccga ccgcgttgtt ggcggcatcg
     1081 tcggagcgaa acgaattgga gcatacgagc tcagcgaggc cgtcggtacg atgcgcctcg
     1141 gtcatccggc cggggcgcat ctcgtgcagg atgacgcgga cgccggcatt ggcgacctgc
     1201 caggccgctt cggaaccggc gaggcccgcg ccgacgatat gcactgattc ggaggaggtt
     1261 ttgatgcctg tcatgggcac aggcgtagcg cgttttccgc tgcgttggaa tgcgcgaagc
     1321 ggcttatagc cccgatacga gcttcgctag gggcgcgcct gtatcggata cgacaacgcc
     1381 cgcagcggtg cgggcgttgt ccgtctcaac agtcggctgg aacggatcag ccgttataat
     1441 tgccggctgc aacgcgcgga atgtccgagc gatcaaggcc gatatcggcc agttcgcgat
     1501 cgctaagctg ggacaattcg ctgacattgc gctggtagtc ccggaacgcc tggatcatgc
     1561 ggatgagcga gagcaacatg gatagtctcc ttaatgtatt cgattcagcc cttggaagcc
     1621 tcatcgttag gaatgaatat agagcagaat attgcactgc agaagttcgt atgttgcgat
     1681 gcagctaagt cctgaatgca tagcagaggt aaggaccgat taacgattcg gcgctgcgcg
     1741 gtcgtgaacc ggccgaaacg gccattctcc agccgaatcc agtaaatatc gcgtgtattc
     1801 acgacattgt cgtagcagcg acagaggacc ttccgtgaac aaaaatgggc attgccgtga
     1861 tggaatcaag gagaatgtcc agaccgaagt cccggtgcgc ctccttcagg gtgattcggc
     1921 ctgtatctca tgcgtgattc gcatgcatga aatgaactta gaagtatttc tttcgccgcg
     1981 agctggcggc gtaccctgac gtcgtcaggc aaatgcggcc ggtgtcctgt taggaattgg
     2041 aaatcaagat gatgctcccg atgtagctag ccggggagga cgcacggaca tggcccacgt
     2101 cataggtcaa ttcgttctcg ccattttgga gagccttgcg gagctgtttt gctttttcac
     2161 cggcaaatgg ttgttgcttc tcatcactgg cggacgggtc gtcccgatgc cgccaacaac
     2221 tccacgaagt tggagtctct cgccattcaa acgactgccg accggacaga ttggcgttga
     2281 ctctcagttt gtggcgctga tcgctttggt attctggatc ctggtcggga tcggtctgca
     2341 ccttttgttt cgctaatcgc accgaggact gagccgtccg gcgttacgaa actgtaatgc
     2401 aaaagcgccg tcgcgcatga ggctctgcac cgtgcgccga tcacatttga atcgcgcgaa
     2461 attcattcct caactttcgg tcaatcggcg aaacaatcgc ttcacagatt ccggcaatga
     2521 cgctggatct gttctaagca aaggaaagta acatgatcaa aaagttactc ggagcagcca
     2581 caatggctgc cgttgcagca tacaccgttg ttcccgcatc tgccgccaag atggccggct
     2641 gcagtggcga aaacatgatg aagaccgaat cgatgatcga agccatggcc gatggtgagg
     2701 gcaagatcgc ggcgcagaag gaaatcgcga tggcgcaaac cgcgatgctc gatggcaaga
     2761 tgggcgcatg cggaatgcat ctaagcaaag cgatgcatgt gagcacgtcg aagtaagctt
     2821 gtcgcagcgt aggcttgcca taggggccga ttgcgtcgcg atcggccctt taggcggtgc
     2881 ccgcaaattg tcagggcgcg tgggcaagct gggttgcgaa atatgcgatc gtcttcgaat
     2941 agacctcgct cttgttccat tgctggatca cgctgaaatt cgcgctgccg ggctcccagt
     3001 ccttgccgcg cttccagccg taactggcaa gataattcgc ggttgacgcc agtacatcgg
     3061 gcgcgctgcg caacaggtcg cggcggccgt tgccgtcaaa atccaccgcg aatttgatgt
     3121 aggacgacgg catgaactgg gtctggccga tttcgccggc ccacgcaccg cgcatttccg
     3181 ttgccgcgag gtcgccgcgc tcgacgatgc gcaaggcgtc catcagctca gccttgaaca
     3241 tgtccgatcg ccggcagtcg taggccagcg tcgccagcga acgcatggtc ggaaactttc
     3301 cgatgttgac gccgaagtcg gtctccaaac cccagatcgc caccagcacc tcgccgggca
     3361 ccccgtagcg ttcttcgatg cggccgagca ccgagccata ttgtttcatc aggctggcgc
     3421 cgcgcgtgag ccggggaggg accatgcggc cagaaaactc ctcaaagctc tgactgaaga
     3481 cccgctgcga atgatcgcgg gacagcaccc cctggtccag cgttaccccg gcgagacccg
     3541 atgcgatcgc agacggcgaa attcccttgg cggcggcttc attcttgaaa tcgtcgagcc
     3601 acgccccaaa gctgccggtg ccgcagggcg ccgcctgcgc cgacgcgacc ggcatcgaca
     3661 ggattgcgct caccgcgagc gttctgaaaa gcaaacgagg ggtcatcggg gccacctgca
     3721 cagaaacgat tcgccatcaa gagtgatgcc cgggcaacat gagatgttgt gagcaacctc
     3781 ggccaaaaca aggcttttga cgacagcatg ttccatgcga cagcgtgatg aaaatatcaa
     3841 cgccggaacg aggccgcgag cgcatcgccg tgatgggcgc ccggcccatt gcacatatcg
     3901 tgcgcatcgc ccggcgatgg cgccgaaaac agcgccacac gcgccaaacc gtcggacaac
     3961 gaacgaccga cggcttggtg gcaaggcttg tcttaaggct tatgcgtcgt ccttgcgccg
     4021 ccagggaaac aaggccgccg ggaaatccgc ggcggagcgc cgttcccgca cccgacgggg
     4081 cggcagcggt tgcggattga gatcgggcgc gatgaccttg cgatagagat gccaggtggc
     4141 gtggccgagc agggggatca ccacggcgag gcccaagaag aacggcagcg atcccaaaac
     4201 cagcagcacc gcgacgatca ggccccatgc cgccatcggc gccgggtttc gcgcgaccgc
     4261 acgtagcgat gtcaccatgg catcgccggc gccggcgtgc cggtcgagca tcaacgggaa
     4321 cgaaaccacg ctgacgcaca gcgccaacac ggcgaacaaa aggccgacgc cgcaaccgac
     4381 gacgatcagc caccaacctt ggggggtcgt cagcacgcgc gtgacgaagt ccggaattcc
     4441 ggcggcagat tcgtagccga aggctgcgac gtaaatggcc tgcgcggtcg cgacccaggt
     4501 cacgaacagc gcgaacaaca acgtgccgag cccgagcatg gcgccgaacg agggcgagcg
     4561 aaatacatcg atcgcatccc atgccgacgc cgtttcgccg cgctcgcggc ggcggctcaa
     4621 ttcataaaga ccgagtgcgg cgaacgggcc gagcaaggca aagcccgcgg cgagcggaaa
     4681 cagcagcggc agcacggagt atcccagcac cgttcgcgcc agcaccagtc cgaggatcgg
     4741 atagatcacg catagaatga tggcgtggct tggcaccgcc ttgaagtcct cccatccgag
     4801 ccgcagtgcg tcatgcagat cggaaagccg gatcgttcgg atcacaggcc cggccgcgct
     4861 atcttgcgca ggctgagcga gtggtccggc attgcctgga tatggggtgc ccatgtcgtt
     4921 gtctcccttg acgcagcggc cgaatttccg cgccaatgaa gcacgcaagc gggccgtgtg
     4981 cagaaacgat cacgatcagg ccagacgcga taacaggaaa cccaagctgg ccatgtcgcg
     5041 aactgaacta ccatcgtact cccaatcatc ggaaccatcg ctcacgcttc ggtgaaagct
     5101 gaattatcgc ggtgcaaaac ctatgcgtcg ctcggacatc attcggcgac cgcaaactca
     5161 ttggcggaac ttccgtgctg cattggcaag cgcatctgcg gcggctaaca ttcggggcga
     5221 tccggtctcc gcgctgccgg caaaacaagc gctgtttggc ccttcacacg atcttcaaat
     5281 tcactgggag caaacgatga gtattttcgg aaaaatcatg agcgcgatct tcggcaccaa
     5341 ggccgacgcg gcaccaccaa gcggtggcgc gacggcaggc acggcgacaa gcgcggggcc
     5401 ggccgccgga tcgtcttccg catcggctac gccggcgcag accgtcgacg tcgcaccgat
     5461 cctggacaag gcggtcgccg ccaagaagga aaagctggaa tggcgcacct cgatcgtcga
     5521 tctgatgaag gcgctcgaca tcgactccag cctcggcgcg cgcaaggagc ttgccaagga
     5581 actcggctac accggcgaca gcaatgattc cgccagcatg aacatctggc tgcacaaaca
     5641 ggtgatgata aaactcgccg ccaacggcgg caagctgccg gcggacatca agcactaagg
     5701 cctgcgggca ctcggttctg agtattcggt gcaatccaaa aggcccgcca atggcgggcc
     5761 ttttgttcac atcacgccgt ggattcggtg cgatggtccg cggcccgtgc cggcgtttcg
     5821 cgcttccggg tcaagacttc ttgctgaacc ccttctcggt gagctgcaac cgtgtgatat
     5881 cgatattaac ccgctggcgc atcacagcgg cagccgattg tcgtacctgc gcccgctgag
     5941 cctgatcgcc actgcgcaag gccagctggt tggccatgtc gctgatcacg aactgatagg
     6001 cgatccgctc cgccatctcc tggcgctcgg cgttcgtcat ggtgcgcagc gccggtgtgc
     6061 cgaggaagat gccgcgcgac tgctggcgcg cgccctcgat ctgcagtttg ttggcggacc
     6121 cgccggtgaa aatttcccag caaagcacca gcagcgccgc catgtcgtct gcgacgttac
     6181 ggctggagta gccgttggcg gacatgacgc ggtcaaattc cttcagcacg acatcgtcag
     6241 cgaaggcttt cgtcatttgc tcgcgcgtcg ccgggttctg accgcccatg gtgtcgatca
     6301 tcgccacgcg gatccgatcg ctttgcgtgg gatcggcggt gtaggtcagc gccgcatcga
     6361 tctgggcggg cgtcaaggaa gccgcccgtg cccgtggagc ggcgtttggg cgcaccgttg
     6421 cgccggcgcc gagccgccgc gtgccgctga ggatcgactg gctgagcgcg atactgccgt
     6481 aaccgcgacc catactcgct gtgaagtgcg gattccactg ggcaacggcg gccgtgctgc
     6541 acagacatat gagcgctgag ccgagcaggg cggctccgcg gccggcccga tgtttgaagc
     6601 tgcgcaccgt aacgcctccc ctggtggcac aaagatcggc gccgcgttgc ggcggtggcc
     6661 acgtgcatcc ccacacgatt ttgattttgt cgctctcaaa gtgtgctttt tttggcaaac
     6721 tgcgggctcc cctgtttatt gcgtgccagt ttattgcgaa catgtgggag cagagtcgaa
     6781 ccgcaacgtc atcgcaaccc cgaagagtga cggagcgccg ttcctcgacg tgctaacgcg
     6841 gagccttgag cacaagcgcg cagcgagagg cgacttttct ggcgatatcg gtgttccaga
     6901 tctcgatcag ggtgcgccac tcgccgcgcg gaattttccc ggcatagacc acggcgagcc
     6961 gatcgccctt cggcgagaac gatgaaaatt ccgcgctgac gtgcgaccag tctgcgggcg
     7021 catcaagcac ggccaccgag tccgttccgc aaatcagctc ggagagcctc tggctgcgga
     7081 caaattcgcc aatccggatc gacgcggccg cgatgacggt gggcaggagc acgacatatc
     7141 ctggatcggc atcgtcgcaa cgcaaccggc gcgtggcggg gacagcaccg ccgacgggca
     7201 ggactgctcg gcaaaccgat ggtcggaaaa cagcccaacc caggccgcgg tgtcggcggc
     7261 agcgatcacg atgatgagtg cgaaacccgt cgacagattc cgccgcagcc tcgacgaatt
     7321 gtttcgataa atccttcgat cgatcgggcg gtcatccgta tcggccgctt cgtcacctcc
     7381 tgccaccgcg acgggcattc ggggacgctc gctactcatt cacgttcact cacgaccaga
     7441 cttcggcgct cacagatggc gaaaccgcat gatcggcaca aatggcgcgg gatcaaggct
     7501 gcacactgtc agaaccgcgg cgcgtcgttt ggtcgaggac ggcatcaacg ttcgcgagag
     7561 cgcgcgaact caacgcgtgg ccgaagactg tgcggtggtc ttaagtggat gcgatctgca
     7621 cccgaaggtc tcgccgcgct tagtgcacga gatcccagga cgtcgctttc agctcgccgc
     7681 ggttgaggcc gatatcgagc aaggtgcggt catcgagctc gtagacgctg gcgagttggg
     7741 agcgatagcg aagatatcgc ccgatggtgt cgaaaagaaa tgcagtgagc atgaccccgg
     7801 tttccctctg agcatgagca tggttcggcg gagggccatt gctcgcattg gtcaggttaa
     7861 cagggcgttg cgccgcagca gcgggaggca ggagtttccc tccatttcca gtaggcttgc
     7921 ggatgtttgg tgaacgcgga gttgccggac ggggcgctgc gggtctacat acgccgtcga
     7981 aaaacgcgga aaacaggagg aaaactaatg gttaccgtcg atcgcaggac gatgctggca
     8041 accggcgcgc tggcgctggc gggcgccgcg gcacagactg gcggcgccga ggcgcaagcc
     8101 ggcccgaagc cgatcttcct ggtgcctgtg atcaccattc cgattgtcgg cgagaccgaa
     8161 gtgtttgccg tgcgccgcat ctactgcatc gggcgcaatt atgccgcgca cgcgatcgag
     8221 cgtggctccg acccgacccg cgagccgccg ttctttttcc agaagccgac cgacgccatc
     8281 cagaacgtcg cgatcggcac ggtcgcggac catccctatc cgttgctgac caagaactat
     8341 caccatgaag tcgaactggt ggcggcgctg aaatccggcg gcaccaatat cgccccggag
     8401 caagcgctcg agcacgtcta tggctatgcg ctaggtctcg acatgacccg gcgcgacctg
     8461 caaaacggca tggccgcgga aaagaagccg tgggaaatcg gcaagagctt tgatcatgcc
     8521 gcggtgctcg ggccgatcca tcccgccgcg aagaccgggc atttcaccaa gggtgcgatt
     8581 tcgctttccg tcaacggcac ggtgcgacag gattccaacc tcgacaagat gatctggagc
     8641 gtggccgagc agatctcaaa actgtccgaa gccttcgagc tgaaggccgg cgacatcatc
     8701 tattccggca cgccggaaaa tgtcggtccg gtggtgaagg gtgatgtgct gttgtgcaag
     8761 ctcgagggcc tgccggatat gtcgatccgg attacgtgag aggaattaca aatttccagc
     8821 gtcgtcccgg cgaaccagcc tgtgtgaaaa gtcgcgtgag ccaaggaagc gcagaattgt
     8881 tttctcaatt gccgtctccc aaccgaggtt gccagtgcaa ttggtttccg tatcgatgaa
     8941 actgagacag atgttctaca cgcaaattga acgacggact tttcacacag tctggaacgc
     9001 cgggacccat aacccctggc gttgatggta aaagaaggca tcagacgcag tgctgaaaac
     9061 gtgagtccgc gccgccgcga tgagcgcaat tgcgctcacg ctggggtccc ggcgttcgcc
     9121 gggacgacgc gaaatcatcc caccaaatcc gcatgctccg gatgcttgcc cagatagtcg
     9181 acgacaaaac cgcagccggc gaccaccttc aatccatcgg cacggatcag ttgcagcgcg
     9241 ccctgcacca gctccgacgc gatgccgcgg ccgcgcagcg cgcgcggcgt ctcggtgtgg
     9301 gtgatgatga ccgtcgacgg cgtcacgcga taattggcaa acgccacccc gccttcgacg
     9361 tcgagttcga agcggttttg tgccttattg tcgcggacgt cggtcatgtc agcaggctca
     9421 ttccaagagg ttggcgtatt cagggtgctt ttcgatatat ggcccccaat caattcttta
     9481 aggagtcggt cagcatgccg cggatcgacc aggcttcgcc gtcgctggcg ccgttgatat
     9541 cgtcgatctt ccattgtccg gcatcgcgga cgagattgta gcggatggtg ttgtcgacgg
     9601 atttggggcg cggcgcgccg cggcccgaga tggtgaccgc gaccacggcc ttgtcggcgt
     9661 caagtttttc gggaaccacc ttgaatgact tgacgttcgg ctcctgcgaa ttggtgacgg
     9721 gatcgaaatc caccggcccg acatcgccct tgggcgtgtg ggcgtcggct ttcgcccata
     9781 attcgaccag cgctttggac aggtattttg cctttgccgc ctggttttcg ataatgaacg
     9841 cgccgccgcc gtcgcccttg cccttcgccg cacgggcgta gatcgcattg atgatggcaa
     9901 cgggatcatt cggggagggt ggcgccgcga ggacagggca cgaaacagcg gctgcaaggc
     9961 cagttgcgcc ggtcaagacc agcgcgcgtc gagtgatcat gattgttgga tccagttgct
    10021 gcgatgcata ggcaaactca ggactcaaag cgagcctcat gaaccacgcg acgaccgtgt
    10081 cggcaacggt gaatcgcggc gtgcaggcat tcccgcgcgc cgttttggcg ttgagccttg
    10141 cgaatgacgg cgctccgtcg ggttatgcat gctatgaacg gcgattgtat catgccatcc
    10201 cggtacgttc gccccgggag acgttcgagc ggctcccgcg acaacatagt ctggaggtca
    10261 tgatggctaa tgaaaaaggc cccaacgtca agaagaagca gaagtgcaag aattgcgagg
    10321 gcaaaggcct gctgaagaag ggcgacaagg tggtcaaatg tcagcgctgc ggcgggacgg
    10381 ggctgcgctg aggctgcttt ggagactgcc ctggcatcca tcacaccaca gttgtcaacg
    10441 ccaagtggcg cgtcccggaa taacagacgc gctcttggac tgaccgtggc ccattaacaa
    10501 tc
//
LOCUS       DASWGM010000623         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_183146,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000623 DASWGM010000000
VERSION     DASWGM010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183146"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGA15_17640"
     CDS             <1..207
                     /locus_tag="VGA15_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9589555.1"
                     /translation="KIFNAERKALQVMGLGPDATLEVVKAKYKALVKQHHPDANGGDR
                     STEDRLIEIIKAYNYLKTVVRGAT"
     gene            562..768
                     /locus_tag="VGA15_17645"
     CDS             562..768
                     /locus_tag="VGA15_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589556.1"
                     /translation="MTAPQRTQFSNSANLVDEMFTEANIDALNAKLIEQGISADRVIA
                     ILPVEAQTMVNPTPPQFRVLYRTA"
     gene            complement(806..1433)
                     /locus_tag="VGA15_17650"
                     /pseudo
     CDS             complement(806..1433)
                     /locus_tag="VGA15_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
     gene            1508..2731
                     /locus_tag="VGA15_17655"
     CDS             1508..2731
                     /locus_tag="VGA15_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HEX9589557.1"
                     /translation="MKKSAALYLSAREASAELAISPATLYAYVSRGLIRSEPSSDSRS
                     HRYRAEDVRGLKERRTPSPEPRGLKSFDADLPVMDSAISTITEDGPIYRGVNCIELAE
                     ADTLEHAATLLWDVTGADPFSSDNCPHVSAEMRGVADAARDAAPIDRTIAVLALAASA
                     DPRAYTRAADGRAMVGGRIMRLVVATMLNGISSPKPLHLQIARAWASDHTQAADLIRR
                     ALVLLADHELNASTFTVRCAASTGLNLYDAVIAGFVALKGPKHGGAGVLAAQFVKTLV
                     DRDVAPVIRERVALGERFAGFGHGVYKHGDPRAHALLEALASAGAAEKLTREIPERIL
                     EATGEFVNIDYALAVLVHTLGLPPGHELVLFAMARTVGWIAHACEQLRHGGLIRPRAR
                     YIGPAPGRSAAFNRP"
     gene            complement(2747..3424)
                     /locus_tag="VGA15_17660"
     CDS             complement(2747..3424)
                     /locus_tag="VGA15_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX9589558.1"
                     /translation="MTSFIDPLIAFVAAHAWLGYLTIFLAALLEAVPVVGSVIPGSTI
                     ILALSALVPGGELRLEGVLAAAIAGALLGDGSAFWIGHRAQREILSSWPMSNYPRVIA
                     QSEAFFHRWGALAVFFARFVPPIRAFVPITAGALGMPPPRFFAVNIPAILLWAPAHVL
                     PGVVAVSALHRYAGIPHHSGIGKHYWMLTVVVGALVVGLTVWAIRRRKGRAMIEPTAE
                     KVRSDFR"
     gene            3721..>4575
                     /locus_tag="VGA15_17665"
     CDS             3721..>4575
                     /locus_tag="VGA15_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9589559.1"
                     /translation="MTTAAMTKAQESVGPAGLPDMKVSVRQVFGIDSDLEVPAYSEVD
                     PHVPDVDTDYRFDRATTLAILAGFAKNRRVMVTGYHGTGKSTHIEQVAARLNWPCVRV
                     NLDSHISRIDLVGKDSIVVRDGMQVTEFRDGILPWALQHNIALVFDEYDAGRPDVMFV
                     IQRVLEVSGRLTLLDQNKVIKPHPAFRLFSTANTVGLGDTSGLYHGTQQINQGQMDRW
                     SIVTTLNYLAHDEEVEIVLAKAHHYRTQEGRDTVNKMVRLADLTRNAFANGDLSTVMS
                     PRTVITWAE"
BASE COUNT          873 a         1493 c         1391 g          818 t
ORIGIN      
        1 aaaatattca acgccgagcg caaggcgctg caggtgatgg gcctcggtcc ggatgcgacg
       61 ctcgaggtcg tcaaggcgaa atacaaggcg ctggtgaagc agcaccatcc cgatgccaat
      121 ggcggcgacc gctcgaccga agatcgcctg atcgaaatca tcaaggcgta taattatctc
      181 aagacggttg tgcgcggcgc aacctgaggc gtctccacgt tgtcgcggcg ttcgccggga
      241 cgactcgtgg aaagactagc gtcgtcccgc cttgcacgac gaccttcggc gtcacgccag
      301 acctatgagg cggcgcatcg cacgccgggc gtcttttcca gttactcaat ctgcatcaag
      361 agcgctggtt gggtcacggc gcatggttcc gtcgatcaga ttttcttatc gttgacgctc
      421 cctattacga ttagacctga tcggaaacct cttctaccgt gtggtcaatc cgcacccagc
      481 gccagcgatc tcccccactg caccggcggc agagtgcgtt cgatcacgac aacaacttca
      541 atatgaaagg aattgtggat catgaccgcg ccccaacgaa ctcagttttc aaacagtgcc
      601 aatctcgtcg atgagatgtt cacggaagcc aacatcgatg ctctcaacgc caagctgatc
      661 gaacagggca tttccgccga tcgggtgatc gccattctgc cggtggaagc acagacgatg
      721 gtcaatccga cgccaccgca gttccgggtg ctgtaccgga ctgcgtgagc gcgcgctcat
      781 attgtctttt catcaagccg tcatctcaaa ccggcatcgg gccgatatag gctgaacttg
      841 gccgtatcag ccggccggtc cgctgctgct cgcgggcgtg cgccgtccat ccggcggcgc
      901 gggccactgc gaagatcggc gtgaaggcct gccgcgggat cgtcagggcg tcgagcatga
      961 tcgcggtgaa gaactccaca ttggtgtcga gcggccggtc cgggcttttc cgccgcagcg
     1021 cttcgcggat ataggcctcc accttgcccg cgaacgatca ccggaaacgc cccgacaatc
     1081 gtcgcctcat gcgaaagccc gttttccgcg cgcaatccgg cgatggcggc ccggaagcca
     1141 tcgacgatcg acaagccctg ggtaatgccg agcaggtcgg caagccgggc ataggcgcgt
     1201 tcgcgcgcca cgcccaatgt ggcgcgcacc tcggcttcac cgactggccg gccggttccg
     1261 ccgctccaca gccgtgcgat cacaccctcg aagccggttt tgcgcacgag gtcgccgacg
     1321 cgttcgccgg cgatgatcag ttcgccacgc tcgccatcga cacggctgag cacggtttcg
     1381 gcggcgggaa tgccctccag cccgatcggg ctcttggaaa gttgcaggtt catggttcgg
     1441 tctcctcccg ggctttcaaa ggtcgaccct ctcgacagat tgatcaatct tgattatgta
     1501 aatcaatatg aaaaaatccg cggcgcttta cctctccgcc cgggaggctt cggcggaact
     1561 ggcgatctcg cccgccacgc tctacgccta tgtcagtcgc ggattgatcc gctcggagcc
     1621 ttcgtcggat tcgcgaagcc atcgctaccg ggcggaggat gtccgcggcc tcaaggaacg
     1681 gcgcacgccg tcgccggagc cgcgcggcct caaaagtttc gacgccgatc tgccggtgat
     1741 ggattcggcg atctcgacca tcaccgagga tggcccgatc taccgcggcg tgaattgcat
     1801 cgagcttgcg gaggccgaca cgctcgaaca tgccgcgacc ttgctgtggg acgtaaccgg
     1861 cgccgatccg ttttcgtccg acaattgccc gcatgtgtcg gcggaaatgc gcggcgtcgc
     1921 cgatgccgcg cgcgatgccg cgccgatcga ccggaccatc gccgtgctgg cgctggccgc
     1981 aagcgccgat ccacgtgcct atacccgagc cgccgatggg cgcgccatgg tcggcggccg
     2041 cattatgcgg ctggtggtcg cgactatgct gaatggcata tcatcgccaa aaccgctgca
     2101 tcttcagatc gctagagcat gggcctccga ccacacgcaa gccgccgatt tgatccggcg
     2161 ggcgctggtg ctgctggccg accatgaact gaacgcctcg acctttaccg tgcgctgcgc
     2221 ggcatccacg ggattgaatc tctatgatgc tgtcattgcc ggcttcgtcg cgttgaaggg
     2281 ccccaagcat ggcggtgccg gagttctggc ggcgcagttc gtcaagacgc tggtcgatcg
     2341 tgacgtggct cccgtcatcc gggagcgcgt tgcgctgggc gagcgttttg cgggtttcgg
     2401 tcacggcgtc tacaagcacg gcgatccgcg cgcgcacgcc ttgctggagg cattggcgag
     2461 cgccggcgcc gcggagaaac tcacccgcga gattccggag cggatccttg aggcgaccgg
     2521 cgaattcgtc aatatcgatt atgcgctggc ggtgctggtg catacgctgg gcttgccgcc
     2581 gggccacgaa ctggtgctgt tcgccatggc gcgcaccgtc ggatggatcg cgcacgcctg
     2641 cgagcaattg cgccatggcg gcctgatccg cccccgcgcg cgctatatcg gtccggcgcc
     2701 ggggcgcagc gcggccttca acaggccatg acgccaaggc cgaatcctat cgaaaatcgc
     2761 ttcgaacctt ctcggcggtc ggctcgatca tggctctgcc ctttcggcga cggatcgccc
     2821 agaccgtcag ccccacgacc agcgcgccga ccaccaccgt cagcatccag tagtgcttgc
     2881 cgataccgct atggtgcgga atcccggcat atcggtgcag cgcggatacc gccaccacgc
     2941 ccggcagcac atgtgccggc gcccacaaca ggatcgccgg aatgttgacg gcgaaaaagc
     3001 gcggcggcgg catgcccaac gcacccgccg tgatcggcac gaaggcgcga attggcggta
     3061 cgaagcgcgc gaaaaacacc gccagcgcgc cccagcgatg gaagaacgct tcgctctgcg
     3121 cgatcacgcg cggatagttc gacatcggcc aggaactcag gatttcgcgc tgggcgcgat
     3181 gtccgatcca gaacgccgag ccgtcgccga gcagcgcgcc ggcgatcgcc gcagccagca
     3241 ccccttcaag gcgcagctcg ccgccgggca ccagcgcgct cagcgcaagg atgatggtcg
     3301 agcccggaat caccgagcct acaaccggaa cggcttccaa cagggcggcg aggaatatcg
     3361 taagatagcc aagccacgca tgcgccgcga cgaacgcaat caagggatcg atgaaggagg
     3421 tcacaaaatt cctgtgctgg tactgccgtc cggtttgggc tcagacctaa cataacttga
     3481 aagcgctgtg agagcccctg cctgaggggg tggctaattg tcccggcgaa agtacggctt
     3541 gattcgcagg gcagccctcc aaaaatcgaa gccagcgcct atcttgatga taacgtgacg
     3601 gaactttgat tgcgccgcgg gcttctgccg gccgttgctc tctgctaggt ttgttccagc
     3661 acccatccgc agccaagccg caaatctggt ttcgggagcg cccgagacct cggaggattg
     3721 atgacgaccg ccgccatgac caaagcgcaa gaatctgtcg gtcccgccgg tttgcccgac
     3781 atgaaggtgt ccgtccggca agtgttcggc atcgatagcg atctcgaagt gccggcctat
     3841 tccgaagtcg atccgcatgt gccggacgtc gatacggatt atcgcttcga tcgcgccacc
     3901 acgctcgcca tcctcgccgg cttcgccaaa aaccgccgcg tcatggtgac gggctatcac
     3961 ggtaccggca agtcgaccca tatcgagcag gtcgcggcgc gactgaactg gccgtgcgtg
     4021 cgcgtcaacc tcgacagcca tatcagccgg atcgacctgg tcggcaagga ttccatcgtc
     4081 gtgcgcgacg gcatgcaggt caccgaattc cgcgacggca ttctgccctg ggcgctgcag
     4141 cacaatatcg cgctggtatt cgacgaatac gacgccggcc gtcccgacgt gatgttcgtg
     4201 atccagcgcg tgctggaagt ctccggccgc ctgacgctgc tcgatcagaa caaagtcata
     4261 aaaccgcatc cggcgttccg gctgttctcg accgccaaca cggtcggcct cggcgatacc
     4321 tccggcctct atcatggcac ccagcagatc aaccagggcc agatggaccg ctggtcgatc
     4381 gtcaccacgc tgaattatct ggcgcatgac gaggaagtcg agatcgtgct cgccaaggcg
     4441 catcactacc gcacgcaaga aggccgcgac accgtcaaca agatggtgcg gctcgccgac
     4501 ctcacccgca acgcctttgc caatggcgat ctgtcgacgg tgatgagccc gcgcacggtg
     4561 atcacctggg cggag
//
LOCUS       DASWGM010000624         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1741751,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000624 DASWGM010000000
VERSION     DASWGM010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741751"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGA15_17670"
     CDS             complement(<1..362)
                     /locus_tag="VGA15_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9589560.1"
                     /translation="MIDAKTGFSPRKFLRGIGVRQVRLACGFVLFSYLLSHFINHSLG
                     NISLDAMEYGLWFHMTWWQSPFGTLLLYPALAVHASLGLWALYQRRHFRWKAIEIVQL
                     AFGLSIPALLCGHLIGQRL"
     gene            complement(535..996)
                     /locus_tag="VGA15_17675"
     CDS             complement(535..996)
                     /locus_tag="VGA15_17675"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9589561.1"
                     /translation="MSTNTRGSMIPTAAVIVAMAFGVAAVTAQSDPIAARQALMKTNG
                     KQAKLGSDMIRGKVRFDLDNAKAIFLSFIDAANKMPNLFPENSKTGGDTSAAPEIWAN
                     MDDFKARFAKLAADSKAALETVKDRESFTAAYRNVNRTCDGCHEHYRVKKS"
     gene            complement(1042..>1534)
                     /locus_tag="VGA15_17680"
     CDS             complement(1042..>1534)
                     /locus_tag="VGA15_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9589562.1"
                     /translation="FDAVLCQFGVMFFPDKEKSYREVHRVLVPRGHYFFSVWDSHRHN
                     PFGRLAHEIIGGFFPADPPQFYQVPFGYHLIDPIKDSLMDAEFTDIEVAVLGVEKTIP
                     DLASFARGLVYGSPVIDQIRERSGVEPDRIVETLADALRREFGADPARMPMQAITFKA
                     RKR"
BASE COUNT          300 a          457 c          484 g          293 t
ORIGIN      
        1 agacgctggc cgatgaggtg cccgcacagc aaggcgggaa tgctcaggcc gaacgccagc
       61 tggacgatct cgatcgcttt ccagcgaaag tgccggcgtt gatagagcgc ccacaatccg
      121 agcgaggcgt gcacggcaag cgccgggtag agcagcagcg ttccgaacgg gctttgccac
      181 cacgtcatgt ggaaccagag cccgtattcc atggcgtcga gcgaaatgtt gcccaaggag
      241 tgattgatga aatgactgag cagatacgag aacagcacga agccgcaggc gaggcgcacc
      301 tgccgcacgc cgatgccgcg caggaacttg cgcgggctga agccggtctt ggcgtcgatc
      361 aagctgtatt ccctcgctga ttccgcacca tagccgcgtt tccgcaacgg ggccagcatg
      421 gggtgttgaa gaggcagctg ccgcgccggc gtgccgccat cgctgcttgt cgtcgcacga
      481 aaccggccgt ccgggcagcg gggttgcggt cccgagccga atcaggagac gcgatcagct
      541 ctttttgacg cggtagtgct cgtggcaacc atcgcaagtc cggtttacat tccgatacgc
      601 ggccgtgaag ctttcgcgat ccttgaccgt ttccagcgct gccttggaat ccgcggccag
      661 cttcgcgaat cgagccttga agtcgtccat attcgcccag atctccgggg ctgcgctggt
      721 atcgccgccg gtcttcgagt tctccggaaa caggttgggc atcttgttcg cggcgtcgat
      781 gaaggacaaa aagatcgcct ttgcgttgtc gagatcgaac cggaccttgc cccggatcat
      841 gtcgctgccg agcttcgcct gcttgccgtt ggttttcatc agcgcctggc gggcggcgat
      901 cgggtcggat tgggcggtca ccgccgcgac gccgaacgcc atcgcgacga tcactgcggc
      961 ggtagggatc atgctccccc gggtgtttgt actcatgttc ccaatctaga ccgccgtcgc
     1021 cgcgttattc ctagccgtac cctagcgctt cctcgccttg aatgtgattg cctgcatcgg
     1081 cattcgagcc ggatcggcgc caaattcgcg gcgaagggca tcggccaagg tttcgacaat
     1141 gcgatcaggc tccacgccgc tacgttcccg gatctgatcg atcaccgggc taccatagac
     1201 cagcccgcgt gcgaaggaag caaggtccgg gatggtcttc tcaacgccga gcacggcaac
     1261 ttcgatatcg gtaaactccg cgtccatcag ggagtccttg atcgggtcga tcaggtggta
     1321 gccgaacgga acctggtaaa attgcggcgg atcggcggga aagaagccgc cgatgatttc
     1381 gtgcgcgagt cggccaaacg gattatggcg gtgtgagtcc caaacgctga agaagtaatg
     1441 gccgcgcgga acaagcacgc ggtgcacctc gcggtaggat ttttccttgt cggggaagaa
     1501 catcacgccg aattggcaca ggacggcatc gaaa
//
LOCUS       DASWGM010000625         5630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1570300,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000625 DASWGM010000000
VERSION     DASWGM010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570300"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VGA15_17685"
     CDS             <1..803
                     /locus_tag="VGA15_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HEX9589563.1"
                     /translation="LAVSFAYGIFHAAGPGHGKAVISSYLVANQETARRGIVLSFASA
                     LMQSLVAVVIVAICAWLLNATAKTMCGAEKAIEIVSYALIAAFGARLVWTKGGGFIRA
                     LQAPRPALAMASAGHGHGHDHHHHHDHDPDQHVHDERGGHSHGPEPSELAGPGGWRRG
                     FGAIFAVGMRPCSGAILVLVFALAQGLFWAGIAATFVMGLGTAITVATIAVVAVSAKD
                     LARRLSAGTEGGGTLVMRGVEFGAAGLVLLFGVGLLFGYLAAERVTCL"
     gene            927..1202
                     /locus_tag="VGA15_17690"
     CDS             927..1202
                     /locus_tag="VGA15_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9589564.1"
                     /translation="MRTLQQHEADLKRLGVEHLYMFGSTARGEANNDSDVDLFFDYQK
                     GKLGVYELMDVKEYAASILGRKTDIMTRDSLHKTLREAIEATAVRVF"
     gene            1202..1561
                     /locus_tag="VGA15_17695"
     CDS             1202..1561
                     /locus_tag="VGA15_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX9589565.1"
                     /translation="MIARSLIPRLTDIIEAIERVNGVLADVSLEAFENDWQRQWLVER
                     GVEIISEASRHLPDDLKARNLEIPWQKVAGIGNVLRHNYESIAAPVLWKLVQADLPTL
                     EKACRSELTAEEGREHR"
     gene            1662..2603
                     /locus_tag="VGA15_17700"
     CDS             1662..2603
                     /locus_tag="VGA15_17700"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9589566.1"
                     /translation="MADIWARKSPAELLSEIAQGDGAGGRAHPLRRTLSALNLVTLGI
                     GGIIGAGIFVLTGHAAAANAGPAVALSFMLGAVACAFAGLCYAEMASAVPISGSAYTY
                     AYATLGELIAWIIGWDLILEYAVGATTVAIGWSGYVVSFAKDFGLVLPAQFVSAPLAF
                     DAATHAWTSTGAVLNLPAAAVIVLMTIILVAGVRETARFNSFLVAVKLVVIVLFVAST
                     VSAFSTANWIAPDNPQGAFIPPNAGPGVYGWSGLVRGAAVVFFAYIGFDAVSTAAQEA
                     RIRCATCRSAFWARGSSARCCMPWSALCSRALFASTN"
     gene            2707..3144
                     /locus_tag="VGA15_17705"
     CDS             2707..3144
                     /locus_tag="VGA15_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX9589567.1"
                     /translation="MSLLGQPRIFRAMAHDGLLPPAFARIHPRFRTPHVSTISTGVVV
                     AILAALLPIDLVGELVSIGTLFAFAVVSLGVLALRIAQPGLDRPFRAPAIWIVAPAGA
                     ATSIFLMFGLPSDTWVRLAVWLAIGLVIYFAYGMRHSRLCSSK"
     gene            3274..3948
                     /gene="tenA"
                     /locus_tag="VGA15_17710"
     CDS             3274..3948
                     /gene="tenA"
                     /locus_tag="VGA15_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEX9589568.1"
                     /translation="MSFFERLKTAASAEWRAYTEHPFTNALADGSLAEAAFRHYLVQD
                     YLFLIEFARAYALSVYKSPRLADMRDAAAGLSAILDVEMNLHVKLCAGWGLSPGDLQQ
                     TAPAVEMLAYTRYVLDAGMRGDLLALKVALAPCVIGYAEIATRLASRPLADAATNPYR
                     VWIAEYAGAAYQEVAAKARAHLEALADLYATPAREAELIVIFKEATRLEADFWEMGWR
                     ADRRAG"
     gene            complement(4099..4503)
                     /locus_tag="VGA15_17715"
                     /pseudo
     CDS             complement(4099..4503)
                     /locus_tag="VGA15_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341488.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            4515..4730
                     /locus_tag="VGA15_17720"
                     /pseudo
     CDS             4515..4730
                     /locus_tag="VGA15_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5250..>5630
                     /locus_tag="VGA15_17725"
     CDS             5250..>5630
                     /locus_tag="VGA15_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HEX9589569.1"
                     /translation="MFLILPNLVMAGPAFEQPTAPQVHEEMWAIPSSPVPMLAYLIRP
                     IGGGGGPFPLVVMNHGVSLDATQRSYFPAIEFRDAALWFARRGYVVVAPVRPGYGATA
                     IEIPERGLFGLFFSGVGKCSDAEFR"
BASE COUNT          944 a         1677 c         1797 g         1212 t
ORIGIN      
        1 tgctcgcggt ttcgttcgcc tatggcatct ttcatgccgc cggccccggc catggcaagg
       61 cggtgatctc gtcctatctg gtcgccaacc aggaaaccgc gcggcgcggc atcgtgctgt
      121 cgttcgcgtc ggcgctgatg cagtcgctgg tcgcggtcgt cattgtcgcg atttgcgcct
      181 ggctgcttaa cgccaccgcc aaaaccatgt gcggtgctga aaaggcgatc gagattgtaa
      241 gctacgcgtt gatcgcggcg ttcggcgccc ggctggtctg gaccaagggt ggcggattca
      301 tccgcgcgct gcaggcgccg cggccggcgc tcgcgatggc gtcggctggt catggtcacg
      361 gccatgatca tcaccatcat cacgaccatg accctgacca gcatgtccat gacgagcgtg
      421 gcggccactc gcacggtcct gagcccagcg aactcgccgg acccggcggc tggcggcgcg
      481 gatttggcgc gattttcgcc gtcgggatga ggccctgttc gggcgcgatc ctggttctgg
      541 tgttcgcgct ggcgcagggg ttgttctggg cgggcatcgc cgcgaccttc gtgatggggc
      601 tcggcaccgc gatcacggtg gcgaccatcg ccgtcgtcgc ggtgtcagcg aaagacctgg
      661 cgcggcgttt gagcgccggg acggaaggcg gcggcacgct ggtgatgcgt ggcgtggaat
      721 tcggcgcagc cggactggtg ctgctgttcg gggtcggcct gctgttcggc tatctcgccg
      781 ccgagcgcgt cacctgcttg tgagaagctc aattgatggc cggttcgcct tcctgtctcc
      841 gcaccgtata ggcctcggcg aaaccgaaag acgtggaaac ggccgctcgg atttgctata
      901 atgttgttat ggaacgcgat gaagccatga ggacattgca gcagcacgaa gccgatttga
      961 agcggcttgg cgtcgaacat ctttacatgt tcggctcgac ggcgcgtggc gaggcgaaca
     1021 acgattccga cgtggacctg tttttcgact atcaaaaggg caagctcggc gtgtacgaac
     1081 taatggatgt gaaggaatac gccgccagca tcctcggccg taagactgac atcatgacgc
     1141 gcgacagcct gcacaagacc ctgcgggaag cgatcgaagc aacagccgtc cgcgtgtttt
     1201 agtgatcgca cgttcgctca tccctcgttt aacggacatc atcgaggcga tcgaacgcgt
     1261 caatggcgta ttggccgatg tgtcgctcga agcattcgaa aacgattggc agcggcaatg
     1321 gctcgtcgag cgcggtgttg agataatttc cgaggcgagt cgtcatcttc cggatgatct
     1381 gaaagcgcgc aatctggaaa tcccttggca gaaagttgcg gggatcggca atgtactgcg
     1441 ccacaactac gagagcatcg ccgcacccgt cctgtggaag ctggtgcaag ccgatttgcc
     1501 gacattggaa aaagcctgcc gctctgaact caccgcggaa gaaggtcggg agcaccgctg
     1561 atatttggac aaggtggggt tcggccgcgc gccgcagccg gataccagcc ggccgttcca
     1621 agacttttcg aaccaattca gcattggaga ttaccatccc gatggcagac atttgggccc
     1681 ggaaatcccc cgccgagttg ttgagcgaaa tcgcgcaagg cgacggcgcg ggtggtcgcg
     1741 cgcatcctct gaggcgaacg ctgtctgcgc ttaacctcgt gacgctcggg atcggcggca
     1801 tcatcggcgc cggcattttc gtgctgacgg ggcatgccgc cgcggccaat gccgggccgg
     1861 cggtagcgct ttcgttcatg ctcggcgcgg tcgcctgtgc gttcgcagga ttgtgctacg
     1921 ccgaaatggc atcggccgtc ccgatcagcg gcagtgccta cacctacgcc tatgccacgc
     1981 tgggcgagtt gatcgcctgg atcatagggt gggacctgat cctcgaatac gccgtgggcg
     2041 ccaccacggt cgccatcggc tggtccggat atgttgtcag ttttgcaaaa gattttgggc
     2101 tggtgcttcc agcccagttt gtttcggcgc cattggcctt cgacgcggca acgcatgcct
     2161 ggacatcgac aggtgccgtg ctgaatctgc cggcggcagc cgtgattgtc ttgatgacaa
     2221 taatactggt ggcgggcgtt cgcgagacgg cgcgcttcaa cagctttctc gtggcggtaa
     2281 agcttgtcgt gatcgtgctg ttcgtcgcga gtaccgtgtc tgccttcagc acggcgaact
     2341 ggatcgcgcc cgataatccg caaggtgcgt tcatcccgcc caatgcaggt cccggggttt
     2401 atggctggtc gggcctggtg cgcggcgcgg ccgtggtgtt ttttgcctat attggattcg
     2461 atgcggtgtc gaccgcggcc caggaggcga gaatccgatg cgcgacgtgc cgatcggcat
     2521 tctgggctcg cgggtcatct gcacggtgct gtatgccgtg gtcggctttg tgctcacggg
     2581 cattgttcgc ttcgacaaat tgaatgttgc cgatccgatt gcggtcggca tcgatgccgc
     2641 cggcgtgggc tggctttcgc cgttcatcaa gctcggaatc atcttcggct gacgtcggtc
     2701 attctggtgt cgttgctcgg ccagccgagg atttttcgcg cgatggcgca tgacggcttg
     2761 ctgccgcctg cctttgccag gatccatccg cggtttcgca caccccatgt ctcgacgatc
     2821 tcgaccggcg tggtcgtggc catcctcgcc gcgcttttgc cgatcgatct ggtgggcgaa
     2881 ctggtcagca tcggaacgct gtttgcattc gccgtggtgt cgctcggcgt tctggcgctg
     2941 cgcatcgcgc agccgggact cgatcgtccc ttccgggcgc cggcgatctg gatcgttgcg
     3001 ccggccggcg ccgcgacgtc gatcttcttg atgttcggcc tgccgagcga tacctgggtg
     3061 aggttggcgg tgtggcttgc gatcggcctc gtgatctatt ttgcctatgg gatgcggcac
     3121 agccggcttt gctcgtcgaa ataggtaacc gctgccttga cgggtgtctg cttttgaggg
     3181 aagagcgaat ggtgttcttg gcctgagagg tctgccgatg accccttagc gagccaaagc
     3241 cgcgcactga gatgcaaacc ctgcgaaaac atcatgagtt tcttcgagcg cctgaagaca
     3301 gcggcatccg ccgagtggcg ggcctacacc gagcacccct tcaccaacgc gttggccgac
     3361 ggttcgctcg ccgaggcggc ttttcgtcac tacctcgttc aggactacct gttcctgatc
     3421 gagtttgccc gcgcctacgc gctctcggtt tacaagtcgc cccggctcgc tgacatgcgc
     3481 gacgcggcgg ccggcctctc cgccattctc gatgtcgaga tgaacctgca tgtaaaactc
     3541 tgcgccggct ggggcctgtc gcccggcgat ctccaacaaa ccgctccggc ggtggagatg
     3601 ctggcctata cgcgctacgt gctcgacgcg ggcatgcgcg gtgatctgct ggcgctgaag
     3661 gtggcgcttg ccccgtgcgt gatcggctac gcagagatcg cgacgcggct cgcctcgcga
     3721 ccccttgcgg acgctgcaac caacccctat cgcgtttgga tcgccgagta tgccggtgca
     3781 gcataccagg aggtcgcggc gaaagcgcga gcgcacctcg aagctctcgc cgatctctac
     3841 gccacgccgg cccgcgaggc agagctgatc gtgatcttca aggaagccac ccggctcgaa
     3901 gccgacttct gggaaatggg ctggcgtgcc gatcggcgtg ctggatagtc gctgaccgcg
     3961 caccgagtgt gctaagcact cgcacgtctc ttcggggggg cgcgatgcgg gttcaccctt
     4021 gcgatatcac tgcagccttc ggcggctctt ccgcgtgccg atgattcggt gagtccgctc
     4081 cgcgcaaact tcccgaactc acccgcgcag ccagcgatgc gccggatcgg catccagccg
     4141 cgggtgccag agcaatgaaa ccgtgatctc cggcgttaag accggaagcg gaaaactatg
     4201 cattccggcg cgcaggtttc cagtgtgtcg ttccggaaca gtggcgatca ggtcggaggc
     4261 cggagctaga gccagcgcgg tcgaaaagcc gcctacaatc gtgacgatct cgcgttccag
     4321 ctccaacggc ttcaaggctt catcaaccgg tcccttgtcg agaccccgcc gcgaaacgga
     4381 gatatgccca ccggccgcat agcgggaggg tgtgatcttg ccctggctca gcgggtgccc
     4441 cattcgcacg acgccaatga agcggtcgcg gaacaatgcc tgctgctcct ggccggaatc
     4501 ttcctgttca cggtaatctc gggctcatca ccggtgcatc cgttatgggc gccgtgttcg
     4561 cagcagcatc ggcgacgatc gacatcacca cggcgcgtcc cgaggctgtt gccaccggta
     4621 tgcggatcac gttcgcggtc gcggcgatac tgatcgtcgt cgcgctcgcc atcgcggttg
     4681 gaagccgtgc tctcgcaaca cgcccttcgc tccctggaga cgttcgataa atgtcggctg
     4741 ttgagagaag agcagacgaa acgctggcgt caccttttgg ctgttaattg acccgattcg
     4801 gacatcgagt gagagtgcgc ctctatgtca ttcgagtgaa atggcggctg cacttaattc
     4861 attggtgagc aatgctgact tgctatccag acaaacggcc gcttaattaa acacgcccta
     4921 cgacagggtt ctgcctgcag tggaggaata aacatgtttc gaatcgccag tttgtcattc
     4981 gcagggttcg cgctgcttat cggagtcgcc atgagtgccg agccagccaa tgccgtcgtc
     5041 tattgcaaga cggttggtgt gccgaggggc tgcgttgttc gaccggctgt tgtggctccg
     5101 gttgtcgtcg cgccggttgc tggccccgtt gtgtattgca agacgcgcgg tgttcccaaa
     5161 ggttgcgtta tgcgatgact gcttgtggca ccaggcggat gatccgtaga gggttgatag
     5221 ccatacgatg ccctttagca agcataatca tgttcctcat cctgccgaat ctggtgatgg
     5281 caggaccggc gttcgagcag cccacagcac cccaggttca cgaggagatg tgggcgattc
     5341 cctcctcgcc tgtgccgatg ctcgcctatc tgatccgacc cattggcggc ggcggcggtc
     5401 catttccact cgttgtgatg aaccatggag tttcgcttga cgcgacgcag cgaagctact
     5461 ttcccgcgat cgaattccgt gacgccgcgc tttggtttgc gcgacggggc tacgtggtgg
     5521 tcgcacctgt acgccctgga tacggtgcta ctgcgatcga aatccccgag cgtggtctgt
     5581 tcgggctttt tttctcgggc gtgggaaaat gttcggatgc tgagtttcgt
//
LOCUS       DASWGM010000626         6138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1016745,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000626 DASWGM010000000
VERSION     DASWGM010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6138
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016745"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VGA15_17730"
     CDS             complement(<1..687)
                     /locus_tag="VGA15_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010971611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9589570.1"
                     /translation="MNIKDLIAIDVHTHAEVSCRQPHDEAWQPYDEAASRYFKVGKRP
                     TIAETIAYYRERKIGLVMFTVDSEFQIGARRIPNEEVADAARENSDIMTAFASIDPHK
                     GRMGVREARSLIEAGVIRGFKFHPTVQGFFPNDRMAYPLYEVIAEHKLPAIFHSGHSG
                     IGTGMPGGGGLHLKYSNPIHLDDVAVDFPDMTIIIAHPSWPWQDEALSVCLHKPNVYI
                     DLSGWSPKYFP"
     gene            complement(684..2792)
                     /locus_tag="VGA15_17735"
     CDS             complement(684..2792)
                     /locus_tag="VGA15_17735"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9589571.1"
                     /translation="MLKDLVQVARQADIAVISVDNPPVNTITAAVRAGLARGLDQVAG
                     LAGVNAVVLRCEGGTFFSGADLGEFNGPPKEAEYRALLGRFEALPVPVIAAMHGTVLG
                     GGLEIALACHYRIAAPGTRFGLPEVTLGIIPGAGGTQRLPRLIGADKALELILGARPV
                     DADEALRYGFLDAVVDGDLGQGGVEYARALLARGRGPRRTSEHSVDPATGTQEIFDRF
                     VAQAKEHYPNRVAPLTAIEAVRASLRLPFGEGLLHEDKLVNGAKTTVESRAAVHVFFA
                     ERGTRIIPDLPETARPRPVRTAAVIGAGTMGVGIAVCFANAAIPVTILDISEESLAQG
                     LDRVDQVYQTMVKRGRLTPDEKQRRMRHIRGTLDYTDLSDADVIIEAIFESMDLKRKI
                     FAALDRVAKPAAVLATNTSTLDVTEIARATKRPQDVVGMHFFSPANVMPLLEVVRTPQ
                     TSAEVIRTIMELSRPLKKTPVLANVCYGFIGNRMMEGYAREAERMVLEGAPPRQIDGA
                     LEQWGMAMGILAVFDMAGIDVGVNVHKANADRYPPDATYYQADFALVEAGRLGQKNGK
                     GYYRYAAGDRTRHDDPEAGAVLRARATELQVPQRRHSAEEIVERCIYPLINEGFRILE
                     ERVALRSADVDVVWTSGYGFPRYRGGPMFHAETLGLETVLAGIEKYRTIFGPMHWQPA
                     PLLVELVRSGRSIADWEHSS"
     gene            2907..3428
                     /locus_tag="VGA15_17740"
     CDS             2907..3428
                     /locus_tag="VGA15_17740"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX9589572.1"
                     /translation="MRQAARRKSDVSQSGEPVTGSIGAGNGGRRIDYGPLRDSIGYAL
                     RRAQVAVFQNFYEAFAELDIRPAQYSVLTIIELNPGVSQTQAADALAIKKTNFVAMID
                     GLEARGLARRTPSENDRRFHALRLTDKGKALMSKLHKIAAAHEGSITQRLGATERRQL
                     CEALRSIAELTAE"
     gene            3540..4001
                     /locus_tag="VGA15_17745"
     CDS             3540..4001
                     /locus_tag="VGA15_17745"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9589573.1"
                     /translation="MDTQQTHAPQPMVSALRALYEPLAPYAYAFMRFCVGATIVPHGY
                     TKLFQGAVAPAAGMIAKLGLEPAIAWAYFVGGVEFFGGVLLAIGLLTRLAAAMLAVEF
                     AVIVFAVKFAIGFFAFKGGFELELLLGLLCLAICFKGGGRLSVDHAIGKEL"
     gene            complement(4145..4405)
                     /locus_tag="VGA15_17750"
     CDS             complement(4145..4405)
                     /locus_tag="VGA15_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589574.1"
                     /translation="MRVYAELFRRPDFNSTERGDHEGFELVIESLGCCQEGRVIVDRD
                     ERRGNDVGDGASAVWTFVEQQLNRHEQSGRRVVAVAWALLLF"
     gene            complement(4422..6095)
                     /locus_tag="VGA15_17755"
     CDS             complement(4422..6095)
                     /locus_tag="VGA15_17755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX9589575.1"
                     /translation="MSTAAKKLSSMDAAFLYLETPETPMHVGSMAIFRPRDGYEGDFF
                     EDFKRTIAERLHLAPILKSRLAETPLHIDHPSWVEDDQFDIDRHIFRASLPAPRDRAM
                     LERTVGWMHAKLLSRARPLWEFYVFEGMQNNEVGLYSRMHHACIDGGAGAALTAIIYD
                     SSPEPRTMTKPVVQVSTAKEPREIATDLLDSYQQMWWQPFDASAGMKGIDLPRTGKND
                     LGSVLFDNAMMQIETLVKFAGSLPAMAKSAAEMLSTVLDPKARESLSSMISPSTILNK
                     SISSERSFAAVSISLSQAKAVAKQAGGKLNDVVMAVSSGALRRYLLERGVLPSRSLTA
                     AVPISLREGGNADANNQVFGMICSLASDVEDPRQRLETIIAQSTKSKEMSLPMRALMP
                     QISNLTMLGAPILVQILALLYTRSNLSDVLPPIANVTISNVPGPKQTLYAAGAELLNI
                     FPVSIATNGLALNVTVQSYRDQLDFGLIAGANVLPDVHRLADMLPEELAVLEQAFGIV
                     PAGSVVEPNAAEHRAPSATKRTPAPRMKRRARPPSRGRRQKRQRAGSTG"
BASE COUNT         1155 a         1922 c         1887 g         1174 t
ORIGIN      
        1 cggaaagtat ttcggcgacc agccggacag gtcgatgtag acgttcggtt tgtggagaca
       61 gaccgaaaga gcttcgtcct gccaaggcca tgacggatgc gcgatgatga tcgtcatgtc
      121 ggggaaatcg accgcgacat catcgaggtg aatcgggttg gagtatttga ggtgcagtcc
      181 gccgccgccc ggcatgccgg tgccgatgcc cgaatggccg ctatggaaga ttgccggcag
      241 cttgtgctcg gcaatcacct catagagcgg atacgccatc cggtcgttcg ggaagaaacc
      301 ctggacggtg gggtggaact tgaagccgcg gatcaccccg gcctcgatca aactgcgcgc
      361 ctcgcgcacg cccatccgtc ccttgtgcgg gtcgatgctg gcaaaggccg tcatgatgtc
      421 gctgttctcg cgggccgcat cggcaacctc ctcgttcgga atccgccgag cgccgatctg
      481 gaattccgaa tcgaccgtga acatcaccaa gccgatcttt ctctcgcgat agtaggcgat
      541 ggtctcggca atggtcgggc gtttcccgac cttgaagtac ctgctggcgg cttcgtcgta
      601 tggctgccac gcctcgtcat gaggctgccg gcaggacact tcggcatggg tgtgaacgtc
      661 gatggcgatc aagtctttga tgttcatgat gagtgttccc agtcggcgat gctgcggccc
      721 gagcgaacca gctcgacgag cagcggtgcg ggctgccaat gcatcggacc gaagatcgtg
      781 cggtatttct cgattcccgc gagcacggtt tcgaggccca aggtttcggc atggaacatg
      841 gggccgccgc gataacgcgg gaagccatag ccgctggtcc aaacaacatc gacgtccgcc
      901 gagcgcagag cgacgcgttc ttccagaatc cggaagccct cgttgatcag cggatagatg
      961 cagcgctcga cgatctcttc cgcgctgtga cggcgctgcg gaacttgcag ctcggttgcg
     1021 cgcgcgcgca gcacggcacc ggcctccgga tcgtcgtggc gggtacggtc gcccgccgca
     1081 tagcgatagt agcccttgcc gttcttctgt ccgaggcgac cggcttcgac caatgcgaag
     1141 tccgcctggt aataggttgc gtcgggcggg tagcgatcgg cgttcgcctt atggacgttg
     1201 acccccacat cgataccggc catgtcgaat acggcgagaa tgcccatcgc catgccccat
     1261 tgctcgagcg caccgtcgat ttggcgcggc ggcgcgcctt ccagaaccat gcgctcggcc
     1321 tcgcgcgcgt agccttccat catccggttg ccgataaagc cgtagcagac gttggcaagg
     1381 accggcgtct tcttcagcgg acgggagagt tccatgatgg ttcggatcac ctcggccgag
     1441 gtctgcggcg tccggaccac ctcgagcagc ggcatcacat tcgcgggcga gaaaaaatgc
     1501 atgccgacga cgtcctgcgg gcgtttggtc gcgcgcgcga tttccgtgac gtccagagtc
     1561 gaagtgttcg tcgcgagcac agccgcgggc tttgccaccc ggtcgagagc cgcgaagatc
     1621 ttccgcttga ggtccatgct ctcgaaaatc gcctcgatga tcacgtcggc atcactgaga
     1681 tcggtataat cgagcgttcc ccgaatatgg cgcatccgcc gttgcttctc gtccggcgtc
     1741 aatcgccccc gcttgaccat cgtctgatag acctgatcaa cgcgatcgag accctgcgcc
     1801 agggattcct cgctgatatc caggatcgtc accgggatcg cagcgtttgc aaagcagacc
     1861 gcgatgccaa cccccatcgt accggcaccg attaccgcgg ccgtccttac cgggcgcggc
     1921 cgcgcggtct ccggcaggtc cggaatgatt cgcgtgccgc gttccgcaaa gaacacatgc
     1981 acggcggcgc ggctttcgac cgtcgtcttc gcgccattga ccagcttgtc ctcgtggagc
     2041 aggccctcgc cgaacggcag gcgcaacgag gcgcgtactg cctcgatcgc cgtcagcggg
     2101 gccacgcgat tgggatagtg ctctttggct tgcgcgacga agcgatcgaa gatctcttgc
     2161 gtgccggtag ccggatcgac ggaatgttca ctggttcggc ggggccccct gccccgcgcg
     2221 agcagggcgc gggcatactc gacgccgccc tgtcccaaat cgccgtcgac gaccgcgtcg
     2281 agaaacccgt agcgcagggc ctcatccgca tcgacgggac gcgcaccgag aatgagctcg
     2341 agcgccttgt ccgcgccgat gaggcgcggc agtcgctgcg tacccccggc acccgggatg
     2401 atcccaagcg tcacctccgg caagccgaag cgcgtgcccg gcgctgcaat ccgatagtga
     2461 caggcgagcg cgatttccag gccgccgccg aggaccgtcc catgcatcgc ggcgatcacc
     2521 ggcaccggga gcgcctcgaa acgtccaagc aacgcgcgat attccgcttc cttcggcggc
     2581 ccgttgaact cgccgagatc ggcaccggaa aagaacgtgc cgccctcaca gcgcagcacc
     2641 acggcgttta cgccggccag ccccgcgacc tgatcgagcc cgcgcgccaa gcctgcgcgc
     2701 accgcggccg tgatcgtatt gaccggcgga ttgtcgaccg agatgacggc aatatcggcc
     2761 tggcgcgcaa cctgaacgag gtccttcaac atatgttctc ccgggaggag tgcgaacatt
     2821 ggttatatta cataacagtt atggcttgtc aaggtgatca aaaggtacta tatccatcga
     2881 gcgaagctgc cccatggagg tgagacttgc gacaagccgc acgaaggaag agcgatgttt
     2941 cccaatcggg agagccggtt acgggctcca ttggcgccgg gaacggcggc cgccgaattg
     3001 attatggccc gcttcgcgac agcatcgggt acgcactcag gcgggcccaa gtcgcggtgt
     3061 ttcagaactt ctatgaagcg ttcgcagagc tggacatccg accggcgcag tattccgtgc
     3121 tgaccatcat cgagctcaat ccaggcgtga gccagacgca agccgccgat gcgctcgcga
     3181 tcaagaagac gaatttcgtc gcgatgatcg atggcctcga ggcgcgaggc ctggctcggc
     3241 gcacgccgag cgaaaatgat cgacgtttcc atgcactgcg cctgaccgat aaggggaaag
     3301 cgctgatgag caagctgcac aagattgccg ctgcccacga gggctcgatc acccaacggc
     3361 tgggcgccac ggagcgcagg cagctgtgcg aagcgcttcg ttcgattgcc gagctaacgg
     3421 ccgagtaacg cagcaacgcc gattcgaatg cgcgaaagcg tttacggcag accggccccg
     3481 tagcgcaggc atcaaaatag agccggtcat caatctcgag atagccaagg agaggtagga
     3541 tggacacgca acaaacgcat gcaccgcagc cgatggtttc ggcgctccgc gcgctctacg
     3601 agccgcttgc gccctatgct tacgccttca tgcgattctg cgtgggggca accatcgtcc
     3661 cgcatggcta cacgaaactc ttccaaggtg ccgtggcccc agccgccggg atgatcgcca
     3721 agctcggtct ggagcccgcg atagcgtggg cgtattttgt cggcggcgtc gagtttttcg
     3781 gcggggtcct gttggcgatc ggcctgctga cccggctggc cgcggccatg ctggccgtcg
     3841 agttcgccgt catcgtgttc gccgtcaaat ttgccatcgg tttcttcgcc ttcaagggcg
     3901 gcttcgagct ggagctgcta ttgggcttgc tctgccttgc gatctgcttc aaaggcggtg
     3961 gcaggttgtc ggtcgatcac gccatcggta aggagctata gtcacctcgc gcttcgcttt
     4021 gcccacccgt gctgtgttgg cgctgtagga tgggtagagc ggaagcgaaa cccacgctgg
     4081 ttttgacgag aggtgatggg ttttcccccg ggtcgagccc ggggtcaacg catcctcccc
     4141 gagttcagaa cagcaggagc gcccaggcca ccgccacgac ccgacggccg gactgttcgt
     4201 gccgattgag ctgttgttca acgaaagtcc agacggcgga tgcgccgtca cctacgtcgt
     4261 tccctcgtcg ctcatcgcga tcgacgataa cgcgcccctc ttggcagcag ccaagcgact
     4321 cgatgacaag ctcgaagccc tcatggtctc cgcgctcggt tgaattgaag tctggtcgcc
     4381 ggaacagttc ggcataaacg cgcaagcgcg ccgccggctg atcagccggt cgagcccgcc
     4441 cgctgccttt tttgccgccg gccccgactg gggggccgcg cgcggcgctt cattcgcggc
     4501 gcgggcgtcc tcttcgtagc cgacggcgcc ctatgttctg ctgcgttcgg ctcgacgacg
     4561 gacccggccg gcacgatccc aaacgcctgc tcgaggacag ccaactcctc gggcagcatg
     4621 tcggccagtc tgtgaacgtc gggcagcaca ttggcgccgg cgatcaggcc gaaatcgagc
     4681 tggtcgcgat agctttgcac cgtgacgttc agagcaagac cattcgttgc gatcgacacc
     4741 ggaaaaatgt tcaacaactc ggcgccggca gcgtaaagcg tttgctttgg cccgggcaca
     4801 tttgaaatcg tgacgttggc aatcggaggc aagacatccg acaggttcga gcgagtgtac
     4861 agcagagcga gaatctgcac tagaatgggc gcgcccagca tcgtcaggtt ggagatttgc
     4921 ggcatcagcg cacgcatcgg aagcgacatt tccttggact tggtcgattg cgcgataatc
     4981 gtctcgagcc tttgcctggg atcttcgaca tcgcttgcca gcgagcaaat catgccgaac
     5041 acttggttgt tcgcgtccgc atttcctcct tcgcgcagcg agatcggaac ggccgcggtc
     5101 agcgagcgcg acggcagaac tccccgttcc agcaggtagc ggcgcagcgc accgctcgac
     5161 acggccatga cgacatcgtt caattttccg ccggcctgct tcgcaacagc cttcgcctgc
     5221 gacagagaaa tcgagacggc agcgaagctg cgctcggatg aaatcgattt attaaggatc
     5281 gtcgacggcg agatcatgct cgacagactt tcgcgcgcct tcggatccaa caccgtcgag
     5341 agcatttccg cagcgctttt cgccatcgcc ggaagacttc cggcgaattt gaccaatgtt
     5401 tcgatctgca tcatcgcatt gtcgaacaga acggaaccga gatcgttctt gccggtgcgc
     5461 ggcaaatcga tgcccttcat ccccgccgac gcatcgaatg gctgccacca catctgctga
     5521 taggagtcca ataggtcggt ggcgatttcg cgcggttcct tggccgtact tacttgtacg
     5581 accggcttgg tcattgttct cggctcggga ctggaatcgt aaatgatggc ggtcagcgcc
     5641 gctccggctc cgccgtcgat gcaggcgtga tgcatcctgg aatagagccc aacttcgttg
     5701 ttctgcatgc cttcaaaaac gtagaattcc caaagcgggc gggctctgct cagcagcttg
     5761 gcgtgcatcc agccgaccgt tcgctccagc atcgcacgat cgcggggcgc cggaagactg
     5821 gcgcggaaga tatggcgatc aatgtcgaac tgatcgtcct cgacccagct cggatgatcg
     5881 atgtgcagcg gcgtttccgc caaccgcgat ttgaggattg gcgcgagatg cagacgctcg
     5941 gcgatcgtcc gcttgaaatc ctcgaagaag tcgccttcgt agccgtcccg cggccggaag
     6001 atcgccatgc tgccgacatg catcggggtc tcgggcgttt ctagataaag aaacgctgcg
     6061 tccatcgagg acagtttttt ggccgccgtg ctcatgagct cctcccggac gcttcgccag
     6121 ttctttaccg aacacact
//
LOCUS       DASWGM010000627        18646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1743503,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000627 DASWGM010000000
VERSION     DASWGM010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18646
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743503"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VGA15_17760"
     CDS             <1..608
                     /locus_tag="VGA15_17760"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9589576.1"
                     /translation="YGNLRSRAMRCLWMLEEVGEPYQLIEKSTRADDLQSAEYLRLNP
                     NARIPALVDGEVVLWESMAINLYLAQKYEGPMHCAGPEVLGLSAQWSFWAVLEIEALL
                     LDLLQHRAVLPEFARDASYAERDELLLRKPLGILNDALAGREYLVGNSFTVADLNVAS
                     ILVWGKIARLDMSTHPEATRWLDGCLARAAYGRVRDRISRK"
     gene            943..2427
                     /locus_tag="VGA15_17765"
     CDS             943..2427
                     /locus_tag="VGA15_17765"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9589577.1"
                     /translation="MKLPRRAFLHLAAGAAALPAASRIAYPQSDPTGTPSEPPVHPLA
                     EGLAAYADGLRYDDIDAATIERVKTHVIDTIGCGIGAFDERPVSICRNVALAVGGNAT
                     IVGTDRRTTPDLASFANGAAFRYFDFNDTYVGRFSVHPSDHIAACLAVAEAERSSAQD
                     LITSIVIAYEVNCRLVDALDITTRGWDPPVMSLPAVALAAGKLMKLGPDRLAQAVNLA
                     VNDHIPMAQTRVQTLSDWKGFADAEAARNAVFAALLARGGLTGPAPIFEGQSGFFKQV
                     SGPANIDPDTFGRRGVPFRIHRCAIKPYPAVIYAQTAIVAGIEVAREVGALDRIAAIE
                     IATTSRGYQRTGSEPEKWSPKTRETADHSLPYITARAMFDGDITNESFAPHMFSDPNI
                     LAFMQKITVSEDPNLTARVGAAPTRVTAILADGQRISREVDYAPGFAERPMNRSEVEG
                     KFRGNVGKRWPQQRTDATLQALWALDQTDDLSVLLGRLSMQANP"
     gene            2539..3324
                     /gene="modA"
                     /locus_tag="VGA15_17770"
     CDS             2539..3324
                     /gene="modA"
                     /locus_tag="VGA15_17770"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9589578.1"
                     /translation="MSYLRIAIAAFALIAAMRTGGTADAAEIRVLSSGSLKAALSQLL
                     ADFQKSSGNTATIEYGPAGAIAGRIQNDDAADVVIVSRSQLQKLESNGKVVQGSLVNI
                     AGIALGVAVRKGAPKPDISNVEAFKRALLSARSIGYRDPTTGSTSGIYTASLLERLGL
                     AQDLKPKLNLDRSEGDVPENVFLGVAKGEIEMQIGQITEIVISPGVDLAGPLPSEIQN
                     TTLMAAGIITTSKAPDAAKALISFISSPSSAAVLKARGFQPVN"
     gene            3476..3976
                     /locus_tag="VGA15_17775"
     CDS             3476..3976
                     /locus_tag="VGA15_17775"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX9589579.1"
                     /translation="MSSVIDDYLVDPKVSLLDKTRMQAQVLVPVLRALRAELGNDKAD
                     AIVKQALRDWSKQLFAAIGDGIEGSPRRKWAAIQSVFGEVSGKEVEVSILRHDKEALD
                     IDVTRCRFAEFFRALGEPELGALLICEADFDIASVGEGEVSLERAQTIMRGAPSCTFR
                     YKFAPR"
     gene            complement(4013..5243)
                     /locus_tag="VGA15_17780"
                     /pseudo
     CDS             complement(4013..5243)
                     /locus_tag="VGA15_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
     gene            complement(5514..6491)
                     /locus_tag="VGA15_17785"
     CDS             complement(5514..6491)
                     /locus_tag="VGA15_17785"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9589580.1"
                     /translation="MTDPECSTTVRFSTADLPENLRVATWREHYSHTVLRADIDPGDD
                     ASFDATVVSRSLPGLQLVSGRFTAARIVRTREMIADGNDDLSLLVNQTGIVTVSARGR
                     EVTLRENDAVLISSSEAVVFDRRSFGESIAIRIPYSILSPAVVDVDDAIMRHISRDAP
                     ALNLLTGYANTLLGDDHAMVSPALRHHVATHMHDLTVLALGATREAADVAGNRGLGAA
                     RLKAAKTYIAENLHHRDISIGTVAAHVGMTPRHLQRLFENDGTTFSAFLLGRRLARAY
                     RMLCEPRSGEKQVSAVAYDVGFGDLSYFNRCFRRLYGATPMDVRAGVLK"
     gene            6492..6728
                     /locus_tag="VGA15_17790"
     CDS             6492..6728
                     /locus_tag="VGA15_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589581.1"
                     /translation="MAHFPSAPVSNIGHHAAMLQVDAELVDRGKIRRFHRSVETGFSL
                     QRSDQNKLNNRFFDIISLTADVARNHFPAAGTMI"
     gene            6874..7005
                     /locus_tag="VGA15_17795"
     CDS             6874..7005
                     /locus_tag="VGA15_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589582.1"
                     /translation="MSERGTDAAKLSIVPGTQQKARREAGLCVEALNRRQPVANAIS"
     gene            complement(6998..7816)
                     /locus_tag="VGA15_17800"
     CDS             complement(6998..7816)
                     /locus_tag="VGA15_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEX9589583.1"
                     /translation="MKRILGGMVVAAALSGSAFAADLGARTYAKAPAAAPVSNWSGLY
                     VGGNLGYGWGSGNTDFSFPAEFGFNNATLGARSSGVIGGAQLGYNWQIGSVVTGLEAD
                     IQGSGIKGSARAPAIPTVVPLVPFESSLSSEHKLSWFGTVRGRLGVTVTPELLLYGTG
                     GLAYGRVDASANSFESFELGGGQAQAQAQAQAQAPASVSKTKIGWTAGAGAEWMFARN
                     WSAKLEYLYVDLGSESAIGDVTVKGPLDPTEAKVGYTWHTRENIARVGVNYHFN"
     gene            complement(8417..10372)
                     /locus_tag="VGA15_17805"
     CDS             complement(8417..10372)
                     /locus_tag="VGA15_17805"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEX9589584.1"
                     /translation="MNDRMAAIAASLLMSAAPALLFSAAPIFVAAPAQAQSFSQMLVF
                     GDSSVDAGFYKALPNPGGGTPFNDAWAAAVAAGAGAPTNAPGLMNSQVLAAYFGLTAN
                     PANQPGGTNFATSGAKDLAINDGTDGGFKKAIPVATQIDSYLAASGGRANGSGLYLIS
                     SGGNDVSFAFGGFPASPKNQADRIAYLQDTANGLADKIAVLHNAGARTIIVPGRSFSF
                     PIGKPEQQAAELAYTHALWSDLAARGVNFIPADFNSVRVAIAADPSAFGFQFIKSDAA
                     STACTEPVVGGVKIRTAYALLCSSNPAAPSHLVSPDAAQTHLLADDQHLTTAGQKLQA
                     DYFYSLIVAPSEISFLAENAVQSRTVTVAGIQDQIGISRQRQTAGFNVWINGDVSSLK
                     IDNPAPGFPGDPSTPLSGTVGLDYKWASGYLLGGAITVGTQTPGFELGGSFRQKEVAG
                     SVYGSVVKGPTWASVIGTYGALRYDVNRIVPIGITMQGSNGTTNGSNVSLAAQAGYDF
                     VNGAWTHGPVAGLTWQQVKVGGFTETGGFTALSFGDQTRDSVISALGYKATYNLGQFR
                     PFVQAVWNHELGPIDRTVRAALTTIDAPSYEMAAVKLGRDWATATVGTTLKLSNAFTG
                     LASFTAQAGQTGVTTYGGRVGVNYRID"
     gene            complement(10900..11925)
                     /locus_tag="VGA15_17810"
     CDS             complement(10900..11925)
                     /locus_tag="VGA15_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9589585.1"
                     /translation="MTDPDSSATVRFSTADLPEKNRVATWREQYGHTVLRVDIEPDDD
                     ASYEASVASRALPGLKLMTGRFLAGRTIRTREMIADGNDDLYLFVNRTGDVTVSARGR
                     ELALGENDAVLTSSGEVTVFHRRSSLGECIAIRIPYSMLSPMVVDVDDAIMRRIPQNT
                     AALKLLTGYANALLGEDHAMTTPAHRHRVATHVHDLAALTLGATLEAADVANGRGMAA
                     ARLRAAKIYTIENICSREISVGGVASHLGVTPRYLQRLFETDGTTFSAFLLGQRLARA
                     YRTLCEPQSLQKQVSTIAYDVGFGDLSYFNRCFRRLYGATPMDIREAAAKQQLESFRI
                     DANRSSW"
     gene            complement(12421..15390)
                     /locus_tag="VGA15_17815"
     CDS             complement(12421..15390)
                     /locus_tag="VGA15_17815"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEX9589586.1"
                     /translation="MKSAKPKLLTGRRALMLAVTIAALAAIDRAEANCDRVTSSASPL
                     INQTVNCIGTTTQNGTTGFGVAEDIGNTINVGSEVQPKASVTGTNVGIQITGSGDFAN
                     DTETVNNFGTISGAFGIAGSSGKVNNKAGATISGTGTGSGFDGFGIQILATGVVANSG
                     TISGTNRGVQIQNGTVTNMSGGAISGGFIGVEILDNGTTGNPALFNAGTIKGGTFAVS
                     FLSVNGFPGELINSDLISATGTAGVAVSFGSSGTVTNSHMIQATATGGIAIQAAGTVT
                     VNNSIGGLITGDKFAVNAATVNVTGNAGTIEATGRDGVAIHDSTVNFTGTVNVTGNAG
                     AIQATGTFGIGIKAGTVIVTGNTGTISGGSQGIVSNNADITNSKLITGDDGGIVANHT
                     AKVANSGTISGTGRFGSGIEADTVIVTGNTGTISGGSFGISSNNADITNSKLITGGDG
                     GIFANHTATVANSGTISGTGTSGIGIEAGTGTVIVTGNTGTISGVSFGILSNNADITN
                     SSRTGTISGNIAIQADGFGGMGSTITTAGAIVSTAGASGTAIKLSAAADTLTLLTGSR
                     IVGVVDMGDVAKVGNDTVNVVTVAPSSKVSSLTTAAALPTFIHFSGVTNYIFSGGGFT
                     GPTVQSGTQLATLDPTALAQADRTLMDFTGGVSSLVQGRLNGVAPSANGAMMAMSYAP
                     ESGGNAGPFAKATDKATGINPAWLSPAPITVWANSFGGQRTQHATDSTLRATSTAFGG
                     AIGIDRKMRPDWLVGAFIGGGSGGLSVDLGSQKVDTDYVFAGAYSRFEWASHFLDFTL
                     QGGNAANKSDRLVLNNLAAGGSERARASYNGWFVSPEVAYGYRQNIGNGYVLTPTARV
                     RYVAGMFDGYSETGSAQGLSVGRRTLQDFEERGELDVSRVTTFFGGDHSLKTNLHGGV
                     IALQRAGDGTINAVLIGQNLSFATPGKGSTVGAVAGAGFDYHINTNVAVFGAVEGVAM
                     SDQSRIVTAKGGVRAAF"
     gene            complement(15784..17823)
                     /locus_tag="VGA15_17820"
     CDS             complement(15784..17823)
                     /locus_tag="VGA15_17820"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HEX9589587.1"
                     /translation="MKSANPKLLTGRHALMLAVTISTLAMVDRAEAKCDPESPVNDKI
                     VTCTGTTDGQNGNGTIGYGTNTDIGNTINVLSGASVTGTLEGVLLHDGTINNDGLIEA
                     TGSNAGAIVAIATVTVNNKSSGTIRANGANTAAIVANQDANVTSSGRIEATGIDGIGI
                     ETGGTATVNNSGAIQASLIGIFGGEAVDSGGDVNLINSGTITATQFAVVSVNAANVIN
                     MRTGTISGNIAILASGADGIGSTITNSGAIISTAGASGTAIQLSGAADTLTLLTGSRI
                     IGAIEMGGGSDVVNFSSGKDVAQLVRLNNFTGTINTSGSAPVVHSATQIASLDPTALA
                     QTDRTLLDFTAGVSSLVQGRLNGVSPSANGAMMAMSYAPENSKAGPFTKAPSNAVGMN
                     GTWANPAPITVWANSFGGQRTQDATASTLRATSTAFGGAIGIDRRVRPDWLIGAFIGG
                     GSSGLSVDLSSQKVDTDYVFGGAYSRFEWASHFLDFTLQGGNAANKSDRLVLNNLAAG
                     GSERARANYNGWFVSPEVAYGFRQDIGNSYVLTPTARVRYVAGMFDGYSETGSAQGLS
                     VGRRTLQNFEERGELDVSRVTTFFGGDHSLKTNLHGGVIAQQRAGDTAINAVLIGQNL
                     AFATPGSGSTVGWVAGAGFDYHTNKNVALFGAVEGTMMSDQSRTITAKGGLRAAF"
BASE COUNT         3662 a         5727 c         5714 g         3543 t
ORIGIN      
        1 tatatggtaa cctcagatct cgagcgatgc gatgcctgtg gatgctcgag gaggtgggcg
       61 agccatatca gctgatagag aaaagtaccc gagccgacga tctgcaaagc gctgaatatc
      121 tacggctcaa tccgaatgca cggattccgg ccctcgtgga tggtgaggtg gtgttgtggg
      181 agtcaatggc catcaacctc tacctcgctc aaaaatacga aggaccgatg cactgcgccg
      241 ggcccgaagt gttgggactg tccgcccaat ggagtttttg ggccgttctg gagatagagg
      301 cgctgctgct tgatttgctg cagcatcgtg ctgttctgcc cgaatttgcc cgcgatgcct
      361 cctatgcgga acgcgacgag ttgctgctaa gaaagccact gggaatcctc aacgatgctt
      421 tggccgggcg cgagtacttg gtcggcaaca gctttaccgt cgccgacctg aacgtggcca
      481 gcattctcgt ctggggcaag atcgcccgcc tggatatgtc gactcatccg gaggcgacac
      541 gatggcttga cggttgcttg gcgcgcgccg cctacggccg agtgcgtgat cggattagcc
      601 gcaagtagtg gcccggcctg gtatcaggat tttctaattg gtgcgcgcgg catctcgctt
      661 cgggtccaat cgcgacgatg tccgagcccc ttccgatcta cacccgataa acagacattc
      721 tcagtatccc gtcggcatgt ctcaaaggtg ccgcactcga acatcatcac tatgaatgga
      781 ttgccgcgtc gaacttgccg cacctctcca tcgccggcgc cccgtcgtgc gataacagac
      841 atcgcaccca agctcagcta ctctgggaac ctatctattc cggttcaaac gctgccagga
      901 gacggtgccg aactctggca tactcgacat tgaggaaacg ccatgaagct ccctcgtaga
      961 gcctttctac atctggcagc aggcgctgcc gcactcccgg ccgcctcgcg gatcgcgtac
     1021 ccgcaaagcg atccgaccgg gacgccgtcg gagccacccg tccatccgct tgcggaaggc
     1081 cttgctgcct atgctgatgg cctgcgctat gacgatatcg acgccgcgac catcgaacgg
     1141 gtcaagacgc atgttatcga tacgatcgga tgtggcatcg gcgccttcga tgagaggccg
     1201 gtcagcattt gccgcaacgt cgcgcttgcg gtcggcggta acgcaaccat tgtcggcacc
     1261 gatcggcgca cgacacctga ccttgcatcc ttcgccaatg gcgccgcttt ccgctacttc
     1321 gacttcaatg acacatatgt gggacgcttt tccgttcatc cgagcgatca tatcgcggca
     1381 tgcctcgcgg ttgcggaggc cgagcggtcc agcgcgcagg atttgattac ctcgatcgtc
     1441 atcgcctatg aagtgaactg tcggttggtc gacgccctcg acatcacgac acgcggctgg
     1501 gacccgccgg tcatgagtct tccggcagtg gcgctcgccg cagggaagct catgaaactc
     1561 ggtcccgacc ggctcgcaca agctgtcaat ctcgccgtca acgaccatat tccgatggcc
     1621 cagacccgag tgcagacgct gtccgactgg aaggggttcg cagacgccga agccgcgcgc
     1681 aatgcggtct tcgccgcact tctggcgcgt ggcggcctga caggcccggc tcccattttc
     1741 gaaggccaat ccggtttctt caagcaggtg tccgggccgg cgaacatcga cccagacaca
     1801 tttggtcgac gaggcgtgcc cttccggatt catcggtgcg ccataaagcc ctatcccgca
     1861 gtgatctacg ctcagacggc gatcgtggcc ggaatcgaag ttgccaggga agtgggggcg
     1921 cttgatcgca ttgcagcgat tgagatcgca acgacgagtc ggggctatca acggaccggc
     1981 agcgaacctg aaaaatggtc gcccaaaacc agggagacgg ccgaccatag cctaccctac
     2041 atcacggcac gggcgatgtt cgacggtgac atcaccaatg agagcttcgc accacatatg
     2101 tttagcgatc caaatatcct cgccttcatg caaaagatca cagtcagcga ggaccccaat
     2161 ctcaccgcgc gggtcggagc agctcccaca cgcgtgacgg cgatcctcgc cgacgggcag
     2221 cgaatttcgc gcgaagtcga ctacgccccg ggcttcgcag aacggccaat gaatagatcc
     2281 gaagtcgaag ggaaattccg cggcaacgtg ggcaagcgtt ggccgcagca gcggacggac
     2341 gccaccctgc aggcattgtg ggcgctcgat cagacggacg atctatctgt cttgctcggc
     2401 cggctctcga tgcaggcaaa cccgtaaggc gccgcgcgct tgtagctttg gatgtcgatg
     2461 ggtcgtataa agtcgaggca tcggcaacat tctttagcca cccggcatgc actaaacttg
     2521 gaacggctgg aggccctcat gagctatcta aggatcgcga tagctgcctt tgctttaata
     2581 gccgcgatgc gcaccggtgg cactgcggat gccgccgaaa tcagggttct gtcatctggc
     2641 tctttgaagg ccgcgctatc ccaactactt gcagactttc aaaagtcatc cggtaacact
     2701 gcaacaattg aatatggtcc agcaggcgca atagctggcc ggattcagaa tgacgatgcc
     2761 gcggatgtgg taatcgtatc cagatctcag ctccaaaagc tcgaaagcaa tggcaaggtc
     2821 gtccaaggga gcctcgtcaa catagcggga atcgcacttg gagtagccgt gcgcaaggga
     2881 gcgcccaaac cggatatcag caatgtcgag gcgttcaaac gggccctgct ctcggcacgg
     2941 tcgattgggt accgcgatcc gacaactggt tcaacgtccg gcatttatac cgccagcctg
     3001 ctcgagcggc tcggccttgc acaagatttg aagccgaagc tcaatcttga caggtcagag
     3061 ggcgatgttc ccgagaacgt cttcctgggg gttgcgaagg gcgaaattga aatgcaaatc
     3121 ggccagataa ccgagattgt tatctcgccc ggcgtcgatc ttgctggtcc gctccctagc
     3181 gagattcaga ataccacttt gatggcagct gggatcatca cgaccagtaa agcgccagac
     3241 gctgccaagg cactcattag ctttatatca tctccctcct ctgccgccgt cttgaaggca
     3301 cgcggattcc agccggtcaa ttagaattaa gcctgattgg ctgccgcgcc ggcggagtcg
     3361 ccgcgtcgaa cttgccgcat ctctccatcg ccggcgcccc gtcgccattt ttcggcgcgg
     3421 tcgtttgtgc tataacagag ccgccagact tgcgacgtcg gtttgtgagg acagaatgag
     3481 cagcgtcatc gacgactatc tcgtggatcc caaggtatcc ctcctggaca agacgcgcat
     3541 gcaggcccag gtcctggtgc cggtgctgcg tgcgttgcgg gccgagctcg gcaacgacaa
     3601 ggccgacgcc atcgtgaagc aggccttgcg cgactggtcg aagcagttgt tcgctgccat
     3661 tggcgacggc atcgagggca gcccgcggcg caaatgggcg gccatccaaa gcgtcttcgg
     3721 cgaggtctcg ggcaaggagg tcgaggtcag cattctgcgc catgacaaag aggcgctgga
     3781 catcgacgtg actcgctgcc gcttcgccga gttcttccgc gcactgggcg agcccgagct
     3841 cggtgcgctg ctgatttgcg aggcggattt cgacatcgcg tcggtcggcg aaggcgaggt
     3901 cagcctcgaa cgcgctcaga cgatcatgcg gggcgcgccg agctgcacct tccgctacaa
     3961 gttcgcaccg cggtaatttc tctgcaaggt cgcagcttgc ggagcacgcg cctcagcggg
     4021 ggcgcgcgac agccagcgtg cgcgccgcga tcaccagcgc cagcaccagc agcgccgacg
     4081 ccagcaggaa cggcgcgccc ggcaatttca gcggcgcctg ggcaccgatg aaataggcga
     4141 atgtcagggt gaacagaaat ggtccgacca gctgggacac gctctgcacg cttgatgtgg
     4201 cgccctgcaa ttggccctgc tggtccggcg cgacccgctg cgtcgtcagc gcctgcaacg
     4261 ccgcacccgc cacaccccac aacgccatca cgggaatgcc gagccagaac cacgggccgg
     4321 tcggtgccgc gccgtacatc agaaagccca gggcgccaca gccgaggccc aataacagcg
     4381 cccggcgctc gccgaagcgc ttgacgatcg gcccgatcgc agccccctgc acggccatcg
     4441 agcagatgcc gacgatggcc agcgtcagac cgaccgttgt cgtatcccaa ccatatcgat
     4501 aggtcgcata gagcacgaag gtagagggca gaaccacatg cgccagttgg gcaaggaagt
     4561 tcaccaccga caaccccgtg agcacccggt cggagcgcaa cagatgcagc gcgcccagcg
     4621 gattcgcgtt tctccagtgg aacggcgaac ggcgatccgg cggcagcgac tccggcagga
     4681 tcaagaggcc gtaaagcgtg ttggcaaagc tcaagcccgc cgcgacccag aacggcaatc
     4741 gcggatccat gcctccgagc agcccgccga ccgccggtcc aagaatgaaa ccggcgccga
     4801 acgccacgcc gatcttgccg aaccgccgcg cgctgttcgg caggcgtcag gtccgcgatg
     4861 taggcgaacg ccgtcgagac gctcgccgaa gtgatccccg agattaccct gccgacgaaa
     4921 agccaggtca gcgacggcgc cagcgccatc agcacgtaat cgagcgcgag cccgaaattc
     4981 gacagcagca ccaccgggcg acggccgaac cggtccgaca gaccgccgag tatcggcgag
     5041 aacagaaact gcatcaccgc ccaggcggtg ccgaacaggc cgaagattcg cgccgcattg
     5101 gcggtgtcgt tgtcgacaaa actttccacc agcttgggca ggatcggcag gatcaggccg
     5161 agcgcgaaca tgtccaacag gatggtgacg aagataaagg ccaccgcgcc gcggcggggc
     5221 gcacccgtca gggacgccgc catgacagtc acgggcggtt cttcaatcat gattgcgctg
     5281 gtgttgatcg gcggatggac tggatacccg gcgcagacta atccaggcac cagcgcagtg
     5341 gcaatgcagg gcaaaccgcg ccggaccagc tcgatagccg atggacccaa aaatcggatc
     5401 ggcgggcttt gggatttgaa agcgacagcg gatagccgga tacgtcgctt cggttacgag
     5461 gcttcggtta cgaggctgct gcaaatttgc agccatccgg ttgcggccag tggctatttc
     5521 aaaacgccgg ctctgacatc catcggtgtg gcgccgtaca gccgtcgaaa gcagcggttg
     5581 aaataggaaa ggtcgccaaa gccaacgtcg taggcgaccg cgctgacctg cttttcgcca
     5641 gaccgcggct cgcagagcat gcggtaggcg cgggcgaggc gccgcccgag cagaaaggca
     5701 gaaaacgtgg tgccatcgtt ctcaaacagt cgctgcagat ggcgcggcgt catgccgaca
     5761 tgcgcggcga cggtgccgat cgaaatatcc cgatggtgga ggttttcggc aatataggtc
     5821 tttgctgctt tcagtcgggc agcccccaac ccccgatttc cggcaacgtc cgcggcttcg
     5881 cgggtagctc cgagcgcaag cacggtcaga tcgtgcatgt gggtcgcaac gtgatgtcga
     5941 agcgcaggcg acaccatcgc gtgatcgtca cccagcaagg tgttcgcgta gcctgtcagc
     6001 aggttgagcg cgggcgcatc ccgggagatg tgacgcatga tcgcatcatc gacatcaacg
     6061 acggccggcg acaatatcga atatggaatt cgaatggcga tggattcgcc gaaggaacgg
     6121 cggtcgaaca cgacggcctc gctgctgctg atcaacaccg cgtcattctc gcggagcgtg
     6181 acttcacggc cgcgcgcaga aacggtgaca atacccgtct gattcaccag cagcgaaaga
     6241 tcgtcgttgc cgtctgcgat catctcgcgc gttcgtacga ttcgggcggc ggtaaatcga
     6301 cccgacacca gctgcagtcc cggcagggaa cgtgaaacca ccgttgcgtc gaacgaggcg
     6361 tcgtcaccgg ggtcgatgtc agcccggagc acggtgtggc tgtaatgctc gcgccaggtg
     6421 gcgacccgca aattttccgg aagatcggcc gtcgagaagc gaactgtcgt ggagcactct
     6481 ggatcggtca cgtggctcat ttcccgtcag cgccagtcag caatatcggc catcatgccg
     6541 caatgctgca ggtcgatgcg gaattggtag atcgcggcaa aattcgccgc ttccaccgat
     6601 cggtggagac cggtttttcg ctgcagcgca gcgatcaaaa taaattgaat aacaggtttt
     6661 ttgacatcat cagcctgacg gcggacgtgg ctcgaaacca ctttccggcc gccggtacaa
     6721 tgatttaggc gcctatcctc gtggcacgtg tgaacgcggc atggccgaag tcaaaaatgg
     6781 cctgacgtca gataaatgac atcggattgg actgcgccgc tggcggctca gccgcggtct
     6841 cacaccaggc gggtacgcta ctggcgcgat tcgatgtcag aacgcggcac ggacgccgcc
     6901 aaactctcga tcgtgcccgg cacacaacaa aaagcccggc gcgaggccgg gctttgcgtt
     6961 gaagcgctga accgacgcca gccggtcgcc aacgcaatca gttgaagtga tagttcacgc
     7021 cgacgcgggc gatgttttcc cgggtatgcc aagtgtatcc gaccttagcc tccgtgggat
     7081 ctagcgggcc ctttaccgta acatctccga tcgcagactc gctgccgagg tcaacgtaaa
     7141 ggtactccag cttggccgac cagttgcgcg cgaacatcca ttcggcgccg gcgcctgcgg
     7201 tccagccgat tttggtcttg ctgacacttg caggtgcttg tgcttgtgct tgtgcttgtg
     7261 cttgtgcttg gccgcctccg agttcgaaag attcgaacga atttgccgaa gcgtcaacac
     7321 ggccataggc cagaccgcca gtaccataga gcaacagctc gggcgtgacg gtgacgccga
     7381 gacggccccg caccgtgcca aaccacgaaa gcttgtgctc ggatgaaaga gaactctcga
     7441 aggggacaag cggaacgact gttgggattg ctggcgcgcg agctgagcct ttgatgcccg
     7501 agccctggat atccgcctct aggccggtca ccacagagcc gatctgccag ttgtatccga
     7561 gctgcgcacc gccgatgacg ccgctcgatc gggcccccaa agtcgcgttg ttgaagccga
     7621 attccgcagg aaacgagaag tctgtatttc cgctgcccca gccataaccg aggttgccgc
     7681 cgacatagag gccgctccag ttgctgactg gtgccgccgc aggcgccttg gcgtaggtgc
     7741 gcgcgccgag atcagcggcg aacgccgaac cggatagcgc cgccgccaca accattccgc
     7801 ccaaaatccg cttcattgaa atctccccga atttgtccgc cgcgcgagcg gcccagcctg
     7861 acgacaggaa tctcacaagc gagattcgat atccgctagt cctatgtgcg acgggaattt
     7921 gcggcttgga cggcggcgaa cgagaatagg acacaagcga cacccagaga ttttgccggc
     7981 gggtgatgcg gattagctac acgccgcgct tacccccagg gctatcgcat atggaatttt
     8041 ctcgtgcctg gtgtttcaac cgattcgtcc ctgcgaacgc agggacccat agccaccggc
     8101 gtcggttgtt atgcaaggcc tctccgctcc gttccgcaac gagacggcac ggcgtatggg
     8161 tccctgcgtt cgcagggacg acccgctgag agcttgcgcg caaccacgac cgcatcaacg
     8221 cacggctcaa ttcagtcata tgcgatagcc ctgccctgac cccggggtag cgccccaaca
     8281 acgggctggc atccaacaga ggtgcaaccg acgacgatcg tttgtgcgct ccggtcagac
     8341 cctgaatatc cgcctcgagg cctgcgacgt acaagcccga gaaccgatag ttatatccga
     8401 cctggctgcc gcatcgtcag tcaatcctgt agttgacacc gacgcgcccg ccataggtgg
     8461 tgacgccggt ctggccggcc tgtgccgtga aagaggcaag cccggtgaac gcattggaca
     8521 gcttcagggt ggttccaacc gttgcggtcg cccaatcgcg acccagcttc accgccgcca
     8581 tctcataact tggcgcgtcg attgtcgtga gcgccgccct cacggtccgg tcgatcggcc
     8641 ccagctcgtg attccacacc gcctgcacga aaggacggaa ttgtccgaga ttgtaagtag
     8701 ccttgtagcc gagcgcagag atgacggagt cgcgggtctg atctccgaac gaaagtgcgg
     8761 tgaagccgcc ggtttcagta aacccgccga ccttgacttg ctgccaggtc aatcccgcca
     8821 ccgggccgtg agtccacgcg ccattgacga aatcgtagcc ggcctgagcg gcgagcgaga
     8881 cgttggaacc gtttgtcgtg ccgttgctgc cctgcatggt gatgccgatc ggcacaatac
     8941 gattcacgtc atagcgcaac gcgccatagg ttccgatcac gctggcccag gtcggaccct
     9001 tgacaacgct gccatagacg ctgccggcga cttccttctg ccggaagcta ccgccgagct
     9061 cgaagccggg cgtttgcgtg ccgaccgtga tcgcgccgcc gagcagatag ccgctagccc
     9121 acttgtaatc gagaccgacg gtaccggaca gcggcgtgct gggatcgccg gggaagccag
     9181 gcgcgggatt atcgatcttg aggcttgaaa catctccgtt gatccagacg ttgaaacccg
     9241 cggtctggcg ctgccgcgaa atgccgatct ggtcttgaat gcctgcgacg gtgacggtgc
     9301 gcgactgcac ggcgttttcc gccaggaatg aaatctcgct cggcgcgacg atcaggctgt
     9361 agaagtaatc ggcctgcagc ttctggcccg ccgtggtgag atgctggtcg tcagctaaca
     9421 agtgagtctg ggcagcgtcc ggcgagacca gatgcgacgg ggccgcagga ttcgaggaac
     9481 acagcagcgc ataggcggtt ctaattttaa cgccgccgac gacgggttct gtacaggccg
     9541 tgctcgcagc atcgctctta ataaactgga agccgaatgc cgagggatcg gctgcgatcg
     9601 cgacccgcac cgagttgaaa tctgccggaa tgaagttgac gcctcgcgcc gccaaatccg
     9661 accacaaagc gtgagtgtaa gcgagttctg ctgcctgctg ctccggcttg ccgattggaa
     9721 acgaaaaact ccggccagga acaataatag tccgcgcacc cgcgttgtgc agaacagcga
     9781 tcttgtccgc caggccgttg gccgtatctt gcagataagc gatcctgtca gcctggtttt
     9841 tagggctggc aggaaaccct ccaaacgcaa aggacacgtc gttaccgccg gagctgatca
     9901 gataaaggcc gctgccgttg gcgcggccgc cactcgccgc aagatagcta tcgatctggg
     9961 tcgcgacagg aatcgccttc ttaaatccgc cgtcggttcc gtcgttgatc gcaagatctt
    10021 tggcgccgct ggttgcaaag ttggtgcccc ccggctggtt ggctggatta gccgtcaacc
    10081 caaaataagc cgccagaacc tgcgagttca tcaatcccgg agcgttggtc ggcgcccccg
    10141 cgccggcagc gaccgcagcc gcccaagcgt cgttaaaggg cgtgccgcca cccggatttg
    10201 gcagggcttt ataaaagccg gcatcgaccg agctgtcacc gaacaccagc atctgactaa
    10261 acgattgggc ctgagccggt gcagccacaa agattggcgc agccgaaaaa agaagggcgg
    10321 gcgcagctga catcagcaag ctcgcggcga tcgccgccat acgatcattc atcgaactat
    10381 ccctagatat ccatgcgtct tccgaatctc agacccgagc ccgtggtggt gactgggcgc
    10441 gcgacggaaa cgaaattcga cgtgcctacg ccttaagcgc cggtgccatt tttgacttgg
    10501 acggaaacga ttttgctttg gacacaactg cagtcgaagc cctttgcggc gtgcccgacg
    10561 catatttgca acatccagca ggtcttgatc ctcgtcggct agagcgtgat ggctttgagt
    10621 tgaatcggcg cgagtgcttg gatcacctcg ccccgcttca acctaaactc atcacgctct
    10681 ggtcggaaaa cacgatcacg acgtctgatc ggaggctgct gcaaattttc agcactccac
    10741 tcgcgggcta gggcagtttt ggatgaaatg acgcgtcatc ttttcctcat ccctgcgtaa
    10801 cggctccgcc gttatcgttg gaggagcggc gtcttcgccg cgtctcgaag gatggagtta
    10861 tggtttcctc ctggttcgag acggcgcaag agcgcctcct caccatgagg agcgattcgc
    10921 atcgatccga aatgactcta attgctgttt cgcagcggct tcccggatat ccatcggcgt
    10981 ggcgccgtag agccgccgga agcagcggtt aaaatacgaa aggtcgccaa agccgacgtc
    11041 gtaggcaatt gtgctcacct gcttttgcag ggactggggc tcgcagagcg tgcggtaggc
    11101 gcgggcgagg cgctggccga gcaaaaatgc ggaaaacgtg gtgccatcgg tctcaaacag
    11161 tcgttgcaga tagcgcggcg tcacgccgag atgcgacgcg acgccgccga ccgaaatttc
    11221 ccggctgcag atattttcga tcgtatagat ctttgctgcc cgtaatcggg ctgccgccat
    11281 cccgcgtccg tttgcaacgt ccgcggcttc gagggtggct ccgagcgtca gcgctgccaa
    11341 atcgtgcacg tgggtcgcaa cccgatgtcg atgcgcaggc gtcgtcatcg catgatcttc
    11401 acccagcaag gcgttcgcgt aacctgtcag aagcttcagc gcggcggtat tttggggtat
    11461 gcgacgcatg atggcatcat cgacatcaac gaccatcggc gacaacatcg aatatggaat
    11521 tcgtatcgcg atacattcgc cgagagaaga acggcggtgg aacacggtga cctcgccgct
    11581 gctggttagc accgcgtcat tctcgccgag cgcgagctcg cggccacgcg cagaaacggt
    11641 aacgtcaccc gtccgattca caaagaggta gagatcgtcg ttgccgtctg cgatcatctc
    11701 acgcgttcgt atggttcggc cggccaaaaa cctccccgtc atcagcttca gtcccggcaa
    11761 ggcacgtgaa gccacagacg cctcgtacga ggcgtcgtcg tcaggctcga tgtcaaccct
    11821 gagcaccgtg tggccgtact gctcgcgcca ggtggcaacc cggttttttt ccggaagatc
    11881 ggcggtcgag aatcgaactg tcgcggagct gtctgggtcg gtcatgcggg ttacatccgg
    11941 taggcgccgg tcatgattgc ggcgatatca gccatcgttc cgcgattttt tggccgctca
    12001 ggccgatgca gtgttgatag gtgaaagcgg gattcgccgc ttccgtcgat cggaggatac
    12061 ctgttattcg gtgcaaagcg cgggcgtggg ctaaccgccc gccgccattt ttgccgtgcc
    12121 cgagagggtt ccgaaattct tgatgaccaa gttcactacc cctcagacgt cacatcgctc
    12181 gcgattcccg caaacggcta gggcctccat ggatactcaa gcaagttcac gctcagatcc
    12241 agaccataga ttgctgcgca cagcgagacc agcaagccca aacagcagat gatgacaatc
    12301 cagatcggca ctggagcgtc ggatgtagtt ttagaagacg gccgttcgtc tggcggcgga
    12361 accgataggc cgctcatctg gatcactccc ttgccgctgc aataagatat tcgtgacgtg
    12421 tcagaacgcg gcgcggacgc cgcccttggc ggtgacgatg cggctctggt cggacatcgc
    12481 gacgccttcg accgcgccga acaccgcgac gttggtgttg atgtgatagt cgaagccggc
    12541 gcccgccact gcgccgaccg tgctgccctt gccgggcgtt gcgaaggaaa ggttctggcc
    12601 gatcagcacg gcgttgatgg tgccatcgcc tgcccgctgc agcgcgatca cgccgccatg
    12661 cagattggtc ttcaagctgt gatcgccgcc gaagaaggtc gtcactctgg acacgtcgag
    12721 ttcgccgcgt tcctcgaaat cctgcagcgt gcggcggccg accgatagcc cctgcgccga
    12781 accggtctca gagtagccgt cgaacatccc tgcaacatag cgcacgcgcg ccgtcggcgt
    12841 cagcacatag ccgttgccga tgttttggcg gtagccgtag gccacttccg ggctgacgaa
    12901 ccagccgtta tagctggcgc gcgcccgctc gctgccgccg gccgcgaggt tgttcagcac
    12961 cagccggtcc gatttattgg ccgcgttgcc gccctgcagc gtgaagtcca ggaaatgcga
    13021 ggcccattcg aagcggctgt aggcgccggc gaacacgtag tcggtatcca ccttctgcga
    13081 gccaagatcg accgacagcc cgccgctgcc gccgccgatg aaggcgccga ccagccagtc
    13141 cggccgcatc ttgcggtcga tgccgatcgc gccgccaaac gccgtcgacg tcgcgcgcaa
    13201 ggtggaatcc gtcgcgtgct gggtgcgctg gccgccaaaa ctgttggccc acaccgtgat
    13261 cggcgccggg ctcagccagg ccgggttgat cccggtagcc ttgtcggtag ctttggcgaa
    13321 gggtccggca ttgccgccgc tctcgggcgc gtaactcatc gccatcatcg cgccattggc
    13381 cgacggcgcc acgccgttga gacgcccctg caccagcgac gacaccccgc cggtaaaatc
    13441 catcagggtg cggtcggcct gcgccagcgc ggtcggatcc agcgtcgcga gctgggtccc
    13501 gctctgcacc gtcgggcccg taaagccgcc gccggagaag atatagttgg tgacgccgct
    13561 gaaatggatg aaggtcggca gcgcagcagc ggtcgtgagc gacgacacct tgctgctcgg
    13621 cgcgacggtc acgacattca cggtgtcgtt gccaactttc gcgacatcgc ccatgtcgac
    13681 cacgccgaca atgcgcgaac ccgtcaatag cgtcagggtg tcggccgcgg cggacaattt
    13741 gatcgcggtg ccgctggcgc cggcggtgct gacgatggcg ccggcagtgg tgatggtgct
    13801 gcccatgccg ccaaagccgt cggcctgaat ggcgatattg cccgagatgg tgccggtgcg
    13861 cgaggagttg gtgatgtcag cattattgct gagaatgcca aatgagacgc cggagatcgt
    13921 gccggtgttg ccggtcacaa tgacggtgcc ggtgccggct tcgatgccga tgccggacgt
    13981 gcctgtcccc gagatggtgc ccgagttcgc caccgtggca gtatgattgg caaagatacc
    14041 cccatcgccc cccgttatga gcttggagtt ggtgatgtca gcattattgc tggaaatgcc
    14101 aaatgagcca ccggagatcg tgccggtatt gccagtcaca atgacggtgt cggcctcgat
    14161 gccggagccg aacctgcctg tccccgagat ggtgcccgag ttcgccacct tggcagtatg
    14221 attggcaacg atacccccat cgtcccccgt tatgagcttg gagttggtga tgtcagcatt
    14281 attgctgaca atgccctgtg agccaccgga gatcgtgccg gtattgccgg tcacaatgac
    14341 ggtgccggct ttaatgccga tgccgaacgt gccggtcgct tgtatcgcgc cggcgttgcc
    14401 ggtgacgttg acggtaccgg tgaagttgac ggtgctgtcg tggatggcga ccccatccct
    14461 gcccgttgct tctatcgtgc cggcattgcc ggtgacattg acggttgcgg cattgacggc
    14521 gaatttgtcg cccgtgatga ggccgccaat ggaattgttc acggtgacag tgccagccgc
    14581 ctggatcgcg atgccgccgg tggcggtcgc ttgtatcatg tgggaattgg ttacggtgcc
    14641 tgaactgcca aaggagacgg cgacgccggc cgtacccgtc gccgagatga ggtcgctgtt
    14701 gatcaattcg ccggggaagc catttacact aaggaagcta accgcgaatg tgccgccctt
    14761 gatggtgccg gcattgaata aagcggggtt gccggttgta ccattgtcca gaatctcaac
    14821 accaataaac ccacctgaaa tcgcgccgcc gctcatgttg gtcacggtgc cgttttgaat
    14881 ttgaacgccg cggttagttc ctgaaatcgt cccggagttg gccacgacgc ccgtagcaag
    14941 gatttggata ccgaacccgt caaaccccga ccctgtccct gtccctgaaa tggtggcgcc
    15001 ggccttgttg ttcactttcc cgctggaacc ggcgatgccg aacgcccccg aaatggtgcc
    15061 gaaattgttg acggtttcgg tgtcgttagc aaaatcacca ctgccagtta tctggatccc
    15121 aacgtttgtt ccagtcaccg acgccttggg ctggacttca gacccgacgt tgatggtgtt
    15181 gccaatatcc tctgcgacgc cgaaaccggt ggtgccgttc tgggtggtcg tgccgatgca
    15241 gttgacggtt tggttgatga gcgggctcgc cgacgaggtg acccgatcac agttcgcctc
    15301 ggcccgatcg atcgccgcca acgccgcgat cgtcaccgcc agcatcagcg cgcgtcgtcc
    15361 cgtcaacagc ttcggtttcg ccgacttcat tatcatctcc agcccaggcc agactcagtt
    15421 cggcgtgtcg ccgacgtcga ccaactgagt agatgggttg gcgattcagg acttggacgg
    15481 aggcgcgggc ggcttggacc caaatgcaca gtcgcgctat tgtcggcggc tgatgcaatt
    15541 ttgcgacacg aacttgcgcc gtcgcggccg gatcggctca accgttgtcg tgatggaacc
    15601 acgcgacccg attgacgcaa gcgtcaggct ggaacgatga cacgggcccg tcattgatat
    15661 ccaatgcttc ggatcgggca tgatgagaac gggacacggg gcaacgctgc gacgatcgtc
    15721 ccatcgcagc gcatcgccct ggcaggcgat atcagctatc gcacttgagc cttctcttcg
    15781 atctcagaac gcggcgcgga ggccgccctt ggcggtgatg gtgcggctct ggtccgacat
    15841 catcgtgccc tcgaccgcgc cgaacagcgc gacgttcttg ttggtgtgat agtcgaagcc
    15901 ggcgccggcg acccagccga cggtgctgcc gctgcccggc gtcgcgaagg cgaggttttg
    15961 gccgatcaag acggcgttga tggcggtgtc gcctgcccgc tgctgcgcga tcacgccgcc
    16021 atgcaggttg gtcttcaggc tgtggtcgcc gccgaagaag gtcgtcactc tggacacgtc
    16081 gagttcgccg cgctcctcga aattctgcag cgtgcggcgg cccaccgata gcccctgcgc
    16141 cgaaccggtc tcagagtagc cgtcgaacat ccctgcgaca tagcgcacgc gtgcggtcgg
    16201 cgtcagcaca taactattgc cgatatcctg gcggaagccg taggccacct ccgggctgac
    16261 gaaccagccg ttgtaattgg cgcgcgcccg ctcgctgccg ccggccgcga ggttgttgag
    16321 caccagccgg tccgatttat tggccgcgtt gccgccctgc agcgtgaagt cgaggaaatg
    16381 cgaggcccat tcgaagcggc tgtaggcgcc gccgaacacg taatcggtgt cgaccttctg
    16441 cgagctcagg tcgaccgaca acccgctgct gccgccgccg atgaaggcgc cgatcagcca
    16501 atccggccgc accctgcggt cgatgccgat cgccccgcca aacgccgtcg aggtcgcgcg
    16561 caaggtggaa gccgtcgcgt cctgggtgcg ctggccgcca aaactgttgg cccacaccgt
    16621 gatcggcgcc gggttcgccc aggtgccgtt catcccaaca gcgttgctgg gcgctttggt
    16681 gaatggcccg gccttgctgt tctcaggcgc gtagctcatc gccatcatcg cgccgttcgc
    16741 cgacggcgac acgccgttga ggcggccctg caccagcgac gacaccccgg cggtgaaatc
    16801 cagcagcgtg cgatcggtct gcgccagcgc ggtcggatcg agcgaggcga tctgggtggc
    16861 gctatggacc accggcgcac tgccgctggt gttgatcgtg ccggtgaagt tgttcagcct
    16921 gaccagctgc gcgacgtcct tgcccgagct aaaattgacg acatcgctgc cgccgcccat
    16981 ctcgatggcg ccaatgatgc gcgaaccagt cagcagcgtc agcgtgtcgg ccgcgcccga
    17041 cagctggatc gccgtgccac tggcgccggc ggtgctgatg atggcgcccg aattggtgat
    17101 ggtgctgccg atgccgtcgg cgccgctggc tagaatagcg atattgcccg agatggtgcc
    17161 agtgcgcatg ttgatcacgt tggcggcatt gaccgacacg acggcaaact gcgttgctgt
    17221 gatcgttccg gaattgatga gattaacgtc gccgccgctg tcaaccgcct cgccaccgaa
    17281 gatgccaatt aaggaggctt gtattgcgcc ggaattgttc acggtcgctg tgccaccggt
    17341 ctcgatacca ataccatcga tgccggtggc ttcaatcctg ccggaactgg tcacgttggc
    17401 gtcttgatta gcaacgatgg cagcggtgtt cgcgccattc gctcgtatcg tgccggagct
    17461 tttattgttc acggtgacgg tggcgatagc aacgatggcg ccggcattgc tgcccgtggc
    17521 ctcgatgagg ccgtcattgt tgatcgttcc gtcatgcagt aggacgcctt cgagggtccc
    17581 cgtcaccgac gcgccggaca gcacgttgat ggtgttaccg atatcggtat tggtgccgta
    17641 gccgatggtg ccgttgccgt tctgaccgtc ggtcgtgccg gtgcaggtga cgatcttgtc
    17701 gttgaccggc gactccggat cgcacttagc ctcggcccga tcgaccatcg ccaacgtcga
    17761 gatcgtcacc gccagcatca gcgcgtgccg tcccgtcaac agctttggat ttgccgactt
    17821 cattatcatc tccagcccag gccagactca gttcggcgtg tcgccgacgt cgaccaactg
    17881 agtagatggg ctggcgattc aggacttgga cggaggcgcg ggcggcttgg acccaaatgc
    17941 acagttgcgc tattgtcggc ggctgatgca attttgcgac acgaacttcg gccgtcttga
    18001 agttgagccg tcctggcctg gatcggctca accgttgtcg ttgcgggatg cacggcgcgg
    18061 ccgggtcgac gcaagcgtca aggccgcatc ctcctccgaa cagatgatga agcgacgcgc
    18121 aggcagcgac gagcttggtc aagcggctcc agtgatttgc ccgccgggca tttttgtgtc
    18181 gagtcccctt tgcaaaaata tttccgtttc caaccacccc aaatcacttc tagaactttt
    18241 cgcatcccac cccaccagag gggcgtatcg cgatcgtcac ggacgtaggg tgggatgcgg
    18301 tggacgcggc agcgttttgc gcgcgaaatg gttgcagggc gggcttcggc ccgtgagcga
    18361 tcaaagagcg tgcggacgag agatgttgca gcgtacggcg aagtcgtgtg gtcctgacgc
    18421 cccgacgctg gcgtcaagtt tgcggaggtt aagtcggccc taccgggctt agacaaaacc
    18481 ttaatccgct gatgacggtg gcaaaaaagc ccggtcaccg gggagagcac gaaggaaacg
    18541 tgtgccgtgg aaaggcacgg ttcttgagag ggtgcaagtc cctcccggca agggtcgctc
    18601 cagccggtag caatcggagc aggcgggaag gcaacgacct gtctga
//
LOCUS       DASWGM010000628         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_908102,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000628 DASWGM010000000
VERSION     DASWGM010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908102"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..382
                     /locus_tag="VGA15_17825"
     CDS             83..382
                     /locus_tag="VGA15_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2285 domain-containing protein"
                     /protein_id="HEX9589588.1"
                     /translation="MRVRPELDPDVDDEAPDGPDITVYDEAHFVTYLRLLDAERDGAD
                     WTEVARIVLHRDPACEELRTRRCFDSHMARAQWMTKRGYRRLLEQSVERARRSRS"
     gene            517..>1890
                     /gene="traG"
                     /locus_tag="VGA15_17830"
     CDS             517..>1890
                     /gene="traG"
                     /locus_tag="VGA15_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020011493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncP-type conjugal transfer protein TraG"
                     /protein_id="HEX9589589.1"
                     /translation="MSGNRILWGQITLVLAVVLATTWTATQWTAWRLGFQPQLGQPWF
                     ELAQSMPVYYPPAFFWWWYAYDAYAPPIFVEGAFIAASGGFIAIAVAITLSILRAREA
                     KNVETYGSARWASNEEIRKAGLLGPDGVILGCHAGHYIRHDGPEHVLCFAPTRSGKGV
                     GLVVPTLLTWPGSCIVHDIKGENWTLTAGFRSRFGRVLKFDPTDALSAAYNPLLEVRR
                     GDKEVRDVQNIADILVDPEGALDRRNHWEKTSHSLLVGAILHVLYAEPDKTLAGVANF
                     LSDSKRPVEATLRAMMSTPHLGAGGVHPVVASAARELLNKSDNERSGVLSTAMSFLGL
                     YRDPVVAKVTDRCDWRIADLIAQPRPLSLYLVVPPSDINRTKPLIRLILNQIGRRLTE
                     ELNAKDRRHRLLLMLDEFPALGRLDFFESALAFMAGYGIKSFLIAQSLNQIEKAYGAN
                     NSVLDNCH"
BASE COUNT          343 a          595 c          568 g          384 t
ORIGIN      
        1 gcttcgcggt gcggttcagc cggttcgact tgcgcccgtt cttgcaggcg tccagaagtc
       61 aaagtggaaa gggaaacgcc tcatgcgggt gagacccgaa cttgatcccg atgtcgatga
      121 cgaagcgcct gatggtcccg atatcaccgt ctatgacgag gcgcatttcg tgacctatct
      181 gcgcctgctc gacgccgaga gggacggcgc tgattggacc gaggtagccc gcatcgtcct
      241 gcatcgcgat cccgcttgcg aggagctaag aacgcggcgc tgcttcgaca gtcacatggc
      301 gcgcgcgcaa tggatgacaa agcgcggcta ccgccgcctg ctggagcaat ccgtcgaacg
      361 agcgcgtcgc tctcgatcct gattgcgaaa attccagaac cccgccgatt tccgcgccat
      421 gctatccggc ggcgcgatcc cggtggtttt ggattgcctt tcgtcagggg gcttattctc
      481 tgatcggttc cgcatcacca gccgatcgga gtcgtcatgt cgggaaaccg tattctctgg
      541 ggccagatca cccttgtctt ggccgttgtg ctggcgacga cctggaccgc aacgcaatgg
      601 acggcttggc ggctcggttt tcagccgcag ctcgggcagc cctggttcga actggcgcag
      661 agcatgccgg tctattaccc gccggcgttc ttttggtggt ggtatgccta cgacgcgtat
      721 gcgccaccga tattcgtcga gggcgccttt atcgcggcgt ccggcggatt catcgccatc
      781 gcggtcgcca tcaccttgtc gatcttaagg gcgcgcgagg cgaagaacgt cgagacctat
      841 ggttcggcgc gctgggcctc caacgaggaa atccgtaagg ccggattgct cggcccggac
      901 ggtgtcatcc tcggctgcca tgccgggcac tatatacgcc atgacggacc ggaacacgtt
      961 ttatgctttg caccgacaag atcgggtaaa ggcgtcggtc tggtggtgcc gacgctgctg
     1021 acctggcccg gaagctgcat cgttcatgac atcaagggag agaactggac gctgacggct
     1081 ggtttccgat cgcgatttgg tcgcgtcctc aaattcgatc cgaccgatgc gctatcagcc
     1141 gcttacaatc cgttgctgga ggtgcggcgc ggcgacaagg aagtgcgcga cgtccaaaac
     1201 atcgccgaca tcctggtcga tccggaaggg gcgctcgatc gccgcaatca ttgggagaaa
     1261 accagccaca gcctcctcgt cggcgcgatc ctgcatgtcc tctatgccga gccagacaag
     1321 acgctcgccg gcgtcgccaa cttcctgtcc gattcgaagc gtccggtcga agcgacgctg
     1381 cgcgccatga tgtcgacacc gcatctcggc gcgggcggcg ttcaccccgt cgtcgcctcc
     1441 gccgcccgcg agctcctgaa caaatcggac aacgaacgct caggcgttct ctctaccgcg
     1501 atgtcgtttc tcgggctcta ccgcgatcct gtggtggcca aggtgacaga ccgctgcgac
     1561 tggcgcatcg ctgatctcat cgcgcaaccg cggccgctca gcctttacct cgtggtgccg
     1621 ccctccgaca tcaaccgcac caagccgctg atccggttga tcctcaacca gattgggcgg
     1681 cggctcacgg aggagttgaa cgccaaagac cgccggcatc gtctgctgct gatgctcgac
     1741 gaattcccag cgctgggaag gttggatttt ttcgagtccg cactcgcctt catggccggt
     1801 tacggcatca agagcttttt gatcgcgcaa tcgctcaacc agatcgagaa ggcctacggc
     1861 gccaacaatt ccgtgttgga caattgccat
//
LOCUS       DASWGM010000629         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_185910,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000629 DASWGM010000000
VERSION     DASWGM010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185910"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGA15_17835"
     CDS             complement(<1..243)
                     /locus_tag="VGA15_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9589590.1"
                     /translation="MTTKRLGVIMNGVTGRMGLNQHLIGSIIAIRDSGGVLLGNGDRM
                     MPDPILVGRDADKVERLAKKFNVTRWTTDLAKALLEP"
     gene            complement(240..1643)
                     /locus_tag="VGA15_17840"
     CDS             complement(240..1643)
                     /locus_tag="VGA15_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589591.1"
                     /translation="MPPSIAVRDIAFFERPVRFIKPFRFGAVVINAASQAFVRVEIEV
                     EGKGTSVGASAELLVPKWFDKRPHLIPEQTVAELRHSLGIARDLYLTGSVFETAFGFH
                     ASRIAAQVEACAREDIPPLAAAYGPAEIDKAILDALLRGLGVNFFDGMAQNIAGIDAR
                     LSPDLRDDDIARFLAGRRRLERVAIRHTVGLDDQVESAGGVADPKENAGARYFKLKLN
                     GDPNADTARLIRIGKQLATLSYDYRVTLDANEQYADLTALGALADRLDHDSALKPIAA
                     KLLYIEQPMPRDITTQAPLGALAMRDFIIDEADDCYEAFPLARALGYRGISSKSCKGI
                     YKSLLNATRAAKWSARGEKYFITSEDLTCQAGLCVQQDLALGALIGVTHAERNGHHYV
                     DGFADTPGAEARAFLAAHPDVYSGDGNKIRLSIHDGDLLTGSLVSPGFATRVHPVWSA
                     LSPLEHPKTKNLQELKL"
BASE COUNT          322 a          519 c          533 g          340 t
ORIGIN      
        1 cggctcaagc agcgccttgg cgaggtcggt ggtccagcgc gtcacattga atttcttggc
       61 gaggcgctcg accttgtcgg cgtcgcggcc caccaggatc ggatccggca tcatgcggtc
      121 gccattgccg agcagcacgc cgccggaatc gcggatcgca atgatcgagc cgatcagatg
      181 ctggttgagc cccatgcggc cggtgacacc gttcatgatc acgccgaggc gtttcgtcgt
      241 catagtttta gctcctgcag gttttttgtt tttgggtgtt cgagcggcga cagcgccgac
      301 cacactggat gcacgcgtgt tgcaaagccg ggcgatacca gcgatcccgt caggagatcg
      361 ccgtcgtgga tcgacagccg aattttattt ccgtcgccgc tgtagacgtc aggatgcgcc
      421 gcgaggaacg cccgcgcttc ggcgccgggc gtatcggcaa agccgtcgac ataatggtgg
      481 ccattgcgtt cggcatgggt gacgccgatc aacgcgccca gcgcgaggtc ctgctgcacg
      541 caaagaccgg cctgacaggt cagatcctcg ctggttatga agtacttttc gccgcgcgca
      601 ctccatttgg ccgcacgggt cgcgttgaga agcgacttgt agatgccctt gcatgatttc
      661 gaagagatgc cgcgatagcc gagcgcgcgg gccagcggga acgcctcgta gcaatcgtcg
      721 gcctcgtcga tgatgaaatc gcgcatcgcc agtgcgccga gcggcgcctg cgtggtgata
      781 tcgcgcggca tcggctgctc gatgtaaagc aattttgcag caatcggttt tagggcgctg
      841 tcatggtcga gacgatcggc cagcgcaccc agcgcggtga gatcggcata ttgctcgttg
      901 gcgtccagcg tgacgcggta gtcatatgac agcgtggcga gttgctttcc aatgcggatc
      961 agtcgcgcgg tatccgcatt gggatcgccg ttaagcttga gcttgaagta gcgggcaccg
     1021 gcattttcct tggggtccgc tactccaccc gcactctcga cctgatcgtc gagaccgacg
     1081 gtatgccgga tcgcaacccg ctccagccgc cggcggcccg cgagaaaccg cgcaatgtcg
     1141 tcatcccgca aatcgggcga caaacgcgca tcgatgccgg cgatattttg cgccatgccg
     1201 tcgaaaaagt tcacgccaag accacgcagc agtgcatcca gtatcgcctt gtcgatttca
     1261 gccggcccgt aagcggcggc gagcggtgga atgtcctccc tggcgcaggc ttcgacctgc
     1321 gccgcgatgc gcgaggcgtg aaagccgaac gcggtttcaa ataccgaacc ggtcagatag
     1381 aggtcgcgcg cgatccccag tgaatgtcgc agctccgcaa cggtttgctc cgggatcaga
     1441 tgcggccgct tgtcgaacca tttcggcacc agcaattcgg cgctggcgcc taccgacgtg
     1501 cccttgcctt ccacctcgat ctcgacgcgc acgaacgcct gcgatgccgc gttgatgacc
     1561 accgcgccga agcggaacgg ttttataaaa cgaaccggac gttcaaaaaa ggcgatgtcg
     1621 cgcacggcga tacttggcgg catggtgact tgtctcaatc cagcgcgctt tgggtgaaga
     1681 aatgttttcg aatccgctgc accagttcgg tgaa
//
LOCUS       DASWGM010000630         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1571188,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000630 DASWGM010000000
VERSION     DASWGM010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571188"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VGA15_17845"
     CDS             <1..1046
                     /locus_tag="VGA15_17845"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9589592.1"
                     /translation="FPDYIPKDRIANWLESYVDIMEINFWTGTTFDGAAYDETAQRWT
                     AHVQRADGTTRTLRPKHIVIATSVNGTPNIPNIETLENFAGKILHSSQFGGGKQWAGR
                     PVVVFGTGTSAHDICQELHAHGAEVTMVQRSPTMVVNVDPSAQLYDQSYLGEGPPIDI
                     RDILNSGIPLAVIKASHKLITNEVKKLDAALLRRLEKVGFRLEFGEDGTGWPLKFRTR
                     GGGYYFNVGCSELIADQKIRLIQATDIKRFTADGLELGDGPTLRADLVVLATGYKGLD
                     HVVASRFGEAVAKRVGPIWGFDDKTQELRNMWTRTPQPGLWFTGGAFSQCRIYSRYIA
                     LQIDAIEAGKLQR"
     gene            complement(1105..1722)
                     /locus_tag="VGA15_17850"
     CDS             complement(1105..1722)
                     /locus_tag="VGA15_17850"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9589593.1"
                     /translation="MPRLNREESQARTRNLLIEAARDEIVKKGFALASVRDIADAAGF
                     SQGAFYSNFPDKEAILLELVQRHQSEERAKIEAALNHADGEPAKALAGIEKWATTINS
                     DPGFAVLAIELQLQALRSRSFAQGYNELNRKHRRALGVLVTKLFELLGRKLPADPVEI
                     ATGFIALGRGMALLSKDGDANRSGRIIVTFLRALIGSAPIETSKK"
     gene            1814..2188
                     /locus_tag="VGA15_17855"
     CDS             1814..2188
                     /locus_tag="VGA15_17855"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX9589594.1"
                     /translation="MPITVITIVGRSLLALLFILAGVAKIAGPQPFLDHMAAHHIPGV
                     LLPLIILLELGAGAALLMGWRLPFAAGAFALFCVATAFVFHLDLADKAERTLFVKDLA
                     IAGALMVIAAGASQIRAVASIG"
BASE COUNT          489 a          756 c          708 g          476 t
ORIGIN      
        1 cctttccgga ctacattccg aaggacagaa tcgcgaactg gctcgaaagc tacgtcgaca
       61 tcatggagat caatttctgg accgggacca cattcgacgg cgccgcgtac gatgagacag
      121 cacaacgatg gacggcgcat gtccaacgag ccgacggcac cacgcgcacg cttcggccaa
      181 aacacatcgt gatcgcgacc agcgtcaacg gcacccccaa cattcccaac atcgagacgc
      241 tggagaattt tgccggcaaa attcttcact ccagtcaatt cggaggcggc aagcaatggg
      301 ccggccggcc ggtggtagtg tttggaaccg gcaccagcgc gcacgacatc tgccaggaat
      361 tgcacgccca tggcgccgag gtcaccatgg tgcagcgcag cccgaccatg gtcgtcaacg
      421 tcgatcccag cgcgcagctt tacgaccagt cctatctcgg cgaggggccg ccgatcgaca
      481 ttcgcgacat cctgaactcg gggattccgc tcgcggtcat caaggcgtcc cacaagctga
      541 tcacgaacga ggtcaaaaaa ctcgatgcgg cgctgttgcg gcgactggag aaagtcggct
      601 tcaggctgga attcggcgaa gacggcaccg gctggcccct aaaattccgg acgcgcggcg
      661 gcggctatta tttcaacgtc ggatgttcgg aactgatcgc ggatcaaaag atccgtctga
      721 tccaggcgac cgacatcaag cgctttacgg ccgatggact ggagctcggg gatggcccga
      781 cgttgcgcgc cgatctggtc gttctggcca ccggctacaa gggcctggat cacgtggtgg
      841 cctcgcgctt cggcgaagcc gtcgccaaac gcgttgggcc gatctggggt tttgatgaca
      901 aaacgcagga gcttcgcaac atgtggaccc gcacgccaca gccgggcctg tggtttaccg
      961 gcggcgcatt ttcgcaatgc cgtatctaca gccgctacat cgcgttgcag atcgacgcca
     1021 tcgaagccgg aaagcttcaa aggtagctga tggcttggag cggcccgttt tcattttcgc
     1081 cacgcggtac agcgaggccg ctgtctactt cttgctggtt tcgatcggcg ccgatccaat
     1141 caaggccctc aagaaggtga cgataatccg gccgctgcgg ttcgcgtcgc cgtcttttga
     1201 caacagcgcc atgccgcggc ccagcgcaat gaacccggtt gcgatctcca ccgggtcggc
     1261 cggcagtttc ctgccgagca gttcaaacag cttcgtcacc agcacgccca gcgcgcggcg
     1321 atgcttgcgg ttcagctcgt tgtagccttg cgcgaaggaa cggctgcgca gcgcctgcaa
     1381 ttgaagctcg atcgccagca cggcaaagcc gggatcggaa ttgatcgttg tggcccattt
     1441 ctcgataccc gccagcgcct tcgcaggctc accatcggcg tggttcaacg cagcctcgat
     1501 cttcgcgcgt tcttcggatt ggtggcgttg cacgagctcg agcaggatcg cctccttgtc
     1561 cggaaaattc gagtagaacg cgccctgcga aaatcccgcc gcatcggcga tgtcgcgaac
     1621 cgaggccaac gcgaagcctt ttttcacgat ctcatcgcgt gccgcctcga tcagcagatt
     1681 tctggtacgc gcctgacttt cctcgcgatt taacctcggc acgaaagcaa cctccaaatc
     1741 acagatacca gttgattttg agatatcaag tgatatcttg aagcatattc ggtaacggac
     1801 aaaggagcac gccgtgccca tcactgtcat tacgattgtc ggccggagcc ttcttgcgtt
     1861 gctgttcatt ctcgctggtg tggcgaagat cgccggcccg caacctttcc tggatcatat
     1921 ggccgcgcac catattccag gcgtattgct tccgctgatc attctgctgg aactgggcgc
     1981 gggcgcggcc ctcctgatgg gctggcggct tccgttcgcc gcaggcgcgt tcgccctgtt
     2041 ttgtgtcgct accgcctttg tgtttcatct cgatctcgcc gacaaggccg agcgaacgct
     2101 gttcgtcaag gacctcgcga ttgcgggggc gctgatggtc atcgcggccg gcgcgtcgca
     2161 aatccgcgcg gtcgcatcga tcggatagtc gcaggccaga tcggctctac gcgttcgaca
     2221 gcaaactgcg cagaaccggt cgcgcaagat ggatcgcgca tgccgcaaca gctccgagcg
     2281 cggcgagaag caggaagaag ctcgtgttcg acaagctgga ccagaagctg ccgatccagc
     2341 cggcggcgat atttccaaca aagctcgtcg tgaaccagat ccccatcaac gtcgagcgcg
     2401 acttgttgga cgccacgttc gataccagc
//
LOCUS       DASWGM010000631         3352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_602368,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000631 DASWGM010000000
VERSION     DASWGM010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602368"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1502
                     /gene="cysS"
                     /locus_tag="VGA15_17860"
     CDS             108..1502
                     /gene="cysS"
                     /locus_tag="VGA15_17860"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX9589595.1"
                     /translation="MDLRLYDTLTKGKRTFVPLDPANVRMYVCGPTVYDFAHIGNARP
                     VIVFDVLFRLLRHIYGENHVTYVRNITDVDDKINDRAARDFPGLPLNEAIRKVTEKTA
                     AQFASDVAALGSLPPTFEPRATDFVLPRADGKTDMVTLIKQLITRGNAYEAGGEVLFD
                     VQSMPDYGALSGRKLDEQRPGARVAVDAHKKSPLDFVLWKQSSGDEPGWESPWGRGRP
                     GWHIECSAMSAAYLGEVFDIHGGGIDLIFPHHENELAQSRCAHGTAAMANYWMHNEFV
                     QVEDAKMSKSLGNFVTIRELLADWPGEVLRLNMLKAHYRSPMDWTLKGLEESAKTLDD
                     WYWVAADNNGERPSDAVIEALSDDLNTPQMIASLHGLRNNAASGSERDRNAFAASLRF
                     LGFLSESAAEWKGRKQQASGVDVEQIDGLIAARATARARKDFKESDRIRGQLAAMGVV
                     IKDSKEGTTWEIAR"
     gene            1499..1663
                     /locus_tag="VGA15_17865"
     CDS             1499..1663
                     /locus_tag="VGA15_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589596.1"
                     /translation="MSPSEIRVIAGLDPAIHQLKNSSAVVWMDARVEPAHDGRGCWQP
                     MRNLYLEEIV"
     gene            1663..2175
                     /locus_tag="VGA15_17870"
     CDS             1663..2175
                     /locus_tag="VGA15_17870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9589597.1"
                     /translation="MGQALPKPALRPFLAADTPMLAAIFVASVEELTGDDYSEAQQQA
                     WASAADDEEQFGKRLASELTLIATLQNSPVGFASLKGTDHIDMLYVHPGAAGQGVASM
                     LCDALEKLAGSRGAKSLSVDASDNAQEFFLKRGYVGKQRNTVTVNGEWLANTTMQKTL
                     VQGPSSGVPT"
     gene            2172..>3352
                     /gene="cimA"
                     /locus_tag="VGA15_17875"
     CDS             2172..>3352
                     /gene="cimA"
                     /locus_tag="VGA15_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX9589598.1"
                     /translation="MTRERLYLFDTTLRDGAQTNGVDFTLHDKQLIAKMLDELGIDYV
                     EGGYPGANPTDTEFFAKKPGLAHARFTAFGMTRRPGRSASNDPGLAALLEARADAICF
                     VAKSSAYQVRVALEITNEENLASIRDSVKAAKSAGREVMLDCEHFFDGFKENAAFALS
                     CAKAAYDSGARWVVLCDTNGGTMPHEVETIVGEVLKHIPGDHVGIHAHNDTEQAVANS
                     LAAVRAGVRHIQGTLNGLGERCGNANLCSLIPTLRLKREFSDCFDIGVSPDKMATLMK
                     VSRTLDDMLNRAPNRHAPYVGESAFVTKTGIHASAVLKDPQTYEHVSPEAVGNRRKVL
                     VSDQAGRSNVIAELERAGIAYEKSDPKLARLVEELKEREAAGYAYESANASFDLLARR
                     TL"
BASE COUNT          666 a         1016 c         1050 g          620 t
ORIGIN      
        1 cgccatttgt ggcatttgcc gcgagattat gagctagcga ttcgctggct gaggccgtct
       61 tttctcattc gagatcactg gacgcccggc cgcaagggac gggatccatg gacttacgcc
      121 tctacgatac cttgaccaag ggaaagcgca ctttcgtgcc gctcgatccc gcgaacgtgc
      181 gcatgtatgt ctgcgggccg acggtctatg atttcgccca tatcggcaac gcccgcccgg
      241 tcatcgtgtt cgacgtcctg ttccgcctgc tgcgccacat ctacggcgag aaccacgtca
      301 cctacgtccg caacatcacc gacgtcgacg acaagatcaa cgatcgcgcc gcgcgtgatt
      361 tccccgggct gccgctgaac gaggcgatcc ggaaagtcac cgagaagacg gccgcacaat
      421 tcgcttctga tgtggcggcc ctgggctcct tgccgccaac cttcgaaccg cgcgccaccg
      481 atttcgtgct gccgcgcgcc gacggcaaaa cggacatggt gaccttgatc aagcaattga
      541 tcacgcgcgg caacgcctat gaagccggcg gcgaagtgct gttcgatgtc cagtcgatgc
      601 ccgattatgg cgcgctgtcg ggccgcaagc tcgacgagca gcggccgggt gcgcgcgttg
      661 ctgtcgatgc gcataaaaag agtccgctcg atttcgtgtt gtggaagcaa tcgtccggcg
      721 atgagcctgg atgggaaagc ccatggggca ggggacgtcc gggctggcac atcgagtgct
      781 cggccatgag cgccgcctat ctcggcgagg tcttcgatat tcacggcggc ggcatcgacc
      841 tgatctttcc gcatcacgag aacgaactgg cgcagtcgcg ctgcgcgcat ggtacggctg
      901 ccatggcgaa ctattggatg cacaacgaat tcgtgcaagt cgaagacgcg aagatgtcga
      961 agtcgctcgg caacttcgtc acgatccggg aattgctggc cgattggccg ggcgaagtgt
     1021 tgcgcctcaa tatgttgaag gcgcattatc gctctccgat ggactggacg ttgaaaggat
     1081 tggaagaaag cgcgaagacg ctcgacgact ggtactgggt ggctgcggat aacaacggcg
     1141 aacggccttc tgacgcagtt atcgaggcac tctcagacga cctcaacacg ccgcagatga
     1201 tcgcctcgct gcacggtttg cgcaataacg ccgcttccgg aagtgaacgg gatcgcaacg
     1261 cgtttgcggc ttccttgcgg tttctgggtt tcttgtccga gagcgcggcg gaatggaaag
     1321 gccgaaaaca gcaagcgagt ggcgtcgacg tagaacaaat cgatggtttg attgccgctc
     1381 gcgccaccgc acgcgcgcga aaagacttca aggaatccga tcgaatccgc ggccaactcg
     1441 cggcgatggg agtagtcatc aaggattcca aggaaggcac cacctgggag attgcgcgat
     1501 gagcccctcc gaaatccgcg tcattgccgg gcttgacccg gcaatccatc aattgaaaaa
     1561 ttcttctgcc gtcgtttgga tggatgcgcg ggtcgagccc gcgcatgacg gacgcggctg
     1621 ttggcagcca atgcgaaacc tctatttgga agagatcgtg taatgggaca ggcgctgccc
     1681 aaaccggctt tgcggccgtt cctcgccgcg gatacgccga tgctggcggc gatcttcgtc
     1741 gcaagtgtcg aggagttgac cggcgacgac tacagcgaag cgcagcagca ggcctgggcc
     1801 agcgccgccg atgacgaaga gcaattcggc aagcgcctcg cctccgagct cacgctgatc
     1861 gctacgctgc agaattctcc ggtcgggttt gcctcgctga agggcacaga tcatatcgac
     1921 atgctttacg tgcatccggg cgcggcgggg cagggcgtgg cgtcgatgct gtgcgacgcg
     1981 ctggaaaagc tcgccggcag ccgcggtgcc aagagtctct cggtcgacgc cagcgacaat
     2041 gcgcaagaat ttttcctaaa gcgcggttac gtcggcaagc agcgcaacac cgtgaccgtc
     2101 aacggcgaat ggcttgccaa caccacgatg cagaagacgc ttgtgcaggg cccgtcgtca
     2161 ggagttccga catgactcgc gaacgcctct atctgttcga caccacgctg cgcgatgggg
     2221 cgcagaccaa tggcgtcgat ttcacgctgc atgacaagca gctgatcgca aagatgctcg
     2281 acgaactcgg tatcgattat gtcgagggcg gctatcccgg cgccaatccg accgataccg
     2341 agttctttgc caaaaagcct ggtctcgctc atgcccgctt cacggcgttc ggcatgacgc
     2401 ggcgtcccgg ccgttcggcc tcgaacgacc cgggtctcgc ggcgctgctg gaggccaggg
     2461 cggatgcgat ctgttttgtc gcgaaatcat cggcgtatca ggtgcgcgtc gcgctcgaga
     2521 tcaccaatga ggaaaatctg gcttcgatcc gcgacagcgt gaaggccgcg aagtcagcgg
     2581 ggcgcgaggt catgctcgac tgcgaacatt tcttcgacgg tttcaaggaa aacgccgcct
     2641 tcgcgctttc ctgcgcgaaa gcagcctatg attccggcgc ccgctgggtg gtgctgtgcg
     2701 acaccaacgg cggcaccatg ccgcacgagg tcgagaccat cgtcggcgag gtgctgaaac
     2761 acattcccgg cgatcatgtc ggtattcacg cccataacga caccgagcag gccgtcgcca
     2821 attcgctggc cgcggtgcgc gccggggtgc ggcacatcca gggcacgctg aacgggcttg
     2881 gtgagcgctg cggcaacgcc aatctgtgtt cgctgatccc gacgctgcgg ctcaagcgcg
     2941 aattttccga ttgcttcgac atcggcgtgt cgccggacaa gatggcgacc ctgatgaagg
     3001 tctcgcggac gctggacgac atgctcaatc gggcgcccaa ccgccacgcg ccctatgtcg
     3061 gggaaagcgc gttcgtcacc aagaccggga ttcacgcctc cgcggtgctc aaggatccgc
     3121 agacctatga gcatgtctcg cctgaagcgg tcggcaatcg tcgcaaggtg ctggtgtccg
     3181 atcaggccgg ccgctccaac gtcatcgccg aactggaacg cgccggcatc gcttacgaga
     3241 agagcgaccc gaaactggct cgcctggtcg aggaattgaa ggagcgcgag gccgcgggct
     3301 atgcctacga gtccgccaac gcgtcgttcg acctgttggc gcggcgcacg ct
//
LOCUS       DASWGM010000632         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2156388,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000632 DASWGM010000000
VERSION     DASWGM010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156388"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1957
                     /gene="leuS"
                     /locus_tag="VGA15_17880"
     CDS             <1..>1957
                     /gene="leuS"
                     /locus_tag="VGA15_17880"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX9589599.1"
                     /translation="QQKMFLDFLRAGLAEREKRKINWDPVDMTVLANEQVIDGRGWRS
                     GAVVEQREMNQWVFKITKYAQELLDALDTLDRWPDKVRLMQRNWIGRSEGMLVRFALD
                     PSTTPAGETELKIFTTRHDTLFGAKFMAIAPDHPLAQAAAAKNPKLAEFIAEAKRIGT
                     AQEIIDTQEKQGFDTGIKAVHPFDENWKLPVYVANFVLMEYGTGAIFGCPAHDQRDLD
                     FVNKYGLGNTPVVCPEGQDPKTFVITDTAYDGDGRMINSRFLDGMTIEQAKEEVAKRL
                     EGEIRPLPNPPPQAGEGRVGAVGERQVNFRLRDWGISRQRYWGCPIPVIHCPTCDVVP
                     VPDKDLPVTLPEDATFDRPGNALDHHPTWKHVACPQCGAKAQRETDTMDTFVDSSWYF
                     ARFTDPWNENAPTTPGVVNRLMPVDQYIGGVEHAILHLLYARFFTRAMKATGHIAFDE
                     PFVGQYTQGMVVHETYKKADGSYATPAEVKIETGGNGRRASLLLTGEEVGIGPIEKMS
                     KSKRNTVDPDDIITTYGADVARWFMLSDSPPDRDVIWSDERVQGASRFVQRLWRLVNE
                     AAEIAKTATSARPATFGSDALVLRKAAHGALDRVSTGIEKLHFNVCLAYIREFSNTLG
                     EVLGREGRPPPDLAWAVGEAAVILVQLV"
BASE COUNT          377 a          624 c          636 g          320 t
ORIGIN      
        1 cagcagaaga tgttcctgga ttttctgcgc gcggggttgg ccgaacgcga gaagcgcaag
       61 atcaactggg atccggtcga catgaccgtg ctcgccaatg agcaggtgat cgacggccgc
      121 ggctggcgtt ccggcgccgt cgtcgagcag cgcgagatga accagtgggt cttcaagatc
      181 acgaaatatg cgcaggaact gctggacgcg ctcgacaccc tggatcgctg gcccgacaag
      241 gtgcggctga tgcagcgcaa ctggatcggg cggtccgagg gtatgctggt gcgcttcgcg
      301 ctcgacccgt ccacgacacc tgccggcgag accgagctga agatcttcac gacccggcac
      361 gacacgctgt tcggcgcgaa gttcatggcg atcgcaccgg atcatccgct ggcccaggcc
      421 gcagccgcca aaaatccaaa actcgcggaa tttatcgcgg aagcaaaacg catcggcacc
      481 gcgcaggaga ttatcgatac ccaggagaag cagggctttg ataccggcat caaggcggtc
      541 catccgttcg acgaaaactg gaagctgccg gtctacgtcg ccaatttcgt gctgatggaa
      601 tacggcaccg gcgcgatctt cggctgcccc gcgcacgacc agcgcgacct cgactttgtc
      661 aataaatacg gtttgggcaa tacgccggtg gtctgtcccg aaggccagga cccgaagacg
      721 ttcgtcatca cggacaccgc ctatgacggc gatggccgca tgatcaattc gcgcttcctc
      781 gacggcatga ccatcgagca ggccaaggaa gaggtggcga agcggctcga gggggagatc
      841 cgccccctcc ctaaccctcc cccgcaagcg ggggagggaa gggtgggggc cgtcggcgag
      901 cggcaggtga attttcggct gcgcgactgg ggcatctcgc gccagcgcta ctggggctgc
      961 ccgatcccgg tgatccactg cccgacatgc gatgtcgtgc cggtgcccga taaggacctg
     1021 ccggtgacgc tgccggagga tgcgacgttc gacaggcccg gcaacgcgct cgaccatcat
     1081 ccgacctgga agcacgtcgc ctgcccgcaa tgcggggcca aggcgcagcg cgaaaccgac
     1141 accatggaca ccttcgtcga ctcgtcgtgg tacttcgcgc gcttcaccga tccgtggaat
     1201 gagaacgcgc cgaccacgcc cggcgtggtg aaccggctga tgcctgtcga ccaatatatc
     1261 ggcggcgtcg agcacgcgat cctgcatctg ctctacgccc gcttcttcac ccgcgccatg
     1321 aaggccactg gccacatcgc gttcgacgag ccgttcgtcg gtcagtacac gcagggcatg
     1381 gtcgtccacg agacctacaa gaaagccgac ggcagctacg ctactccggc cgaggtcaaa
     1441 atcgagaccg gcggcaacgg ccgccgcgcc agtctgctcc tgaccggcga ggaggtcggc
     1501 atcggcccaa tcgaaaaaat gtcgaagtcg aagcgcaaca ccgtcgaccc cgacgacatc
     1561 atcacgacct atggcgccga cgtggcgcgc tggttcatgt tgtcggattc gccgccggac
     1621 cgtgacgtga tctggagcga cgagcgggtg cagggcgcct cgcggttcgt gcagcggctg
     1681 tggcggctgg tcaacgaggc ggctgagatc gccaagactg cgacatcagc ccggccggca
     1741 acgttcggtt ccgacgcgct tgtgcttcgc aaggcggcgc atggggcgct cgacagggtt
     1801 tccaccggca tcgaaaagct gcatttcaac gtctgccttg cctacatccg ggaattttcc
     1861 aatacgctgg gcgaggtgct gggccgcgag gggcggccgc cgccggatct cgcctgggcc
     1921 gtgggcgagg ccgcggtcat cctggttcaa ttggtcg
//
LOCUS       DASWGM010000633         3090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_749243,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000633 DASWGM010000000
VERSION     DASWGM010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749243"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGA15_17885"
     CDS             complement(<1..308)
                     /locus_tag="VGA15_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEX9589600.1"
                     /translation="MTGSHDHTHADHSHDHDDAARWKHDGVRVIPGDQLDPNVPSTAG
                     MDRKAAINFARAGAQKLWAGTVTIKPDAKTGAHHHGHLESIIYVVKGKARMRWGEHLQ
                     "
     gene            465..569
                     /locus_tag="VGA15_17890"
                     /pseudo
     CDS             465..569
                     /locus_tag="VGA15_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042410.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
     gene            complement(760..1737)
                     /locus_tag="VGA15_17895"
     CDS             complement(760..1737)
                     /locus_tag="VGA15_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX9589601.1"
                     /translation="MQISILDDYFDTLRTLKCFGKLAGHDVTVWNDHVQDVDTLAERL
                     GDTEALVLIRERTQIRNALLERLPKLKLISQRSVYPHIDIDACTRLGIVVSSSQHADT
                     PSYATAEFTWGLVLAAMRAIPQQMASLKAGNWQIGVGHTLRGKTLGIYGYGRIGAVVA
                     GYGKAFGMEVLVWAREPALAKARADGYATATSKAAFFEQCDVLSLHMRLVDATRGVVT
                     AADLARMKPTALMVNTSRAPLIEPNALVNALRAGRPGMAAVDVYEKEPLRDTNDPLLT
                     MDNVVCTPHLGYVSRDEYEIQFTDIFDQITAYAAGTPTNVVNPDAIGKR"
     gene            complement(1783..2172)
                     /locus_tag="VGA15_17900"
     CDS             complement(1783..2172)
                     /locus_tag="VGA15_17900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX9589602.1"
                     /translation="MPPSVLAQTAGKTMTTPSGLQITDSTVGTGATPKPGQICVMHYT
                     GWLYEGGKKGKKFDSSVDRNEPFEFPIGQRKVIAGWDEGVATMKVGGKRTLIIPPALG
                     YGARGAGGVIPPNATLMFDVELLDVKG"
     gene            complement(2293..>3090)
                     /locus_tag="VGA15_17905"
     CDS             complement(2293..>3090)
                     /locus_tag="VGA15_17905"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9589603.1"
                     /translation="MLVMLNILSRFDLTKFPPMSVERFHLEAEAARIAYMMREQYIGD
                     PAFVDVDVVRILTKEFAEEYGSKIRMDRLLDLPNVSPPMNPSTVYVTVVDRDRNVCSF
                     INSIAHAFGSAIVSNKTGILLQNRGGGFRVQPGHPNCIAPGKRPLHTIIPSLVTKAGR
                     AVMPFGVMGGQYQPVGQVHVLTNVLDYGCDVQEAIDMPRGLHYEGVYQLEDGVPADIV
                     DGLRKLGHRTASVVTPHGGGQAIWIDWDKGTLTGGSDPRKDGCALGY"
BASE COUNT          590 a          941 c          949 g          610 t
ORIGIN      
        1 aactgcagat gctcacccca gcgcatccgc gccttgccct tcacgacata gatgatgctt
       61 tcgagatggc cgtggtgatg cgcgccggtc ttggcgtccg gtttgatggt cacggtgccc
      121 gcccataatt tctgcgcacc ggcgcgggca aaattgatcg cggccttgcg gtccatgccg
      181 gcggtcgagg gcacgttggg gtcgagctgg tcgccgggaa taacgcggac gccatcgtgc
      241 ttccagcgcg ccgcgtcgtc gtgatcgtgt gaatgatcgg cgtgggtatg atcgtgagaa
      301 cctgtcatcg tgatactttc cgagaaaaat cgttcgctgc aaaaggtaca taatacggtg
      361 atcgcagacc agccgggaaa aggaactttt ggcggatttg ggacctgtct tggtgaagct
      421 caatcgagtg aaggaaaaat tcaatgggtg caacagcaga cgccattcag gaaatcaaag
      481 gcaagggcca gaaggccatg ggcgacgcca aggaagccac caaggacgcc atcaacaaga
      541 ccgccgccgc ggcgaacaag aatctctgat cgacgctggt cgatttgaag tcgaagaccg
      601 gtcccttggg ccggtctttc tcgtttaaat ataatgcgct tcatttcgtc atggccgggc
      661 ttgtcccggc catccacgtt ttatgcgcgg tcatcaagaa agacgtggat gcccgggaca
      721 agcccgggca tgacgatgtg gggccgctcc gcggcgactt caccgttttc ctatcgcatc
      781 cggattgacg acattggtcg gcgtaccagc ggcataggct gtgatctggt cgaaaatatc
      841 cgtgaactgg atttcgtatt cgtcgcgcga gacgtagccg aggtgcggcg tgcaaacgac
      901 attgtccatg gtcagcagcg gatcgttggt atcgcgcagc ggctctttct catagacgtc
      961 caccgccgcc atgccgggtc gcccggcgcg caacgcgttc accagcgcgt tcggttcgat
     1021 cagtggcgca cggctggtgt tgaccatgag cgcggtcggc ttcatgcgtg cgagatcggc
     1081 cgccgtcacg acgccgcgcg tcgcgtcgac caggcgcatg tgcagcgaca gtacatcgca
     1141 ctgttcgaaa aatgcagcct tgctggtcgc ggtcgcgtaa ccgtcggcgc gcgccttcgc
     1201 cagcgccggc tcacgcgccc acaccagcac ctccatgccg aacgccttgc catatccggc
     1261 gacgaccgcg ccgatccggc cgtaaccgta gatgccgagc gtcttgccgc gcagtgtgtg
     1321 gccgacgccg atctgccaat tgccggcttt cagcgacgcc atctgctgcg gtatagcgcg
     1381 catcgccgcc agcaccagcc cccaggtgaa ttcggccgtg gcgtaagacg gcgtatccgc
     1441 gtgctggctg gacgacacga cgatgccgag ccgggtgcag gcatcgatgt cgatgtgggg
     1501 atagacgctg cgctggctga tcaatttcag cttcggcaat cgctccagca gggcgttgcg
     1561 aatttgggtt cgctcgcgga tcagcaccag cgcctcggtg tcgccgagcc gctccgccag
     1621 cgtgtcgacg tcctggacgt gatcgttcca gaccgtgacg tcatgtcctg cgagtttgcc
     1681 gaaacatttc agggtacgca gggtgtcgaa ataatcgtcg aggatggaaa tctgcacgat
     1741 acgttttccc tggcacagaa cttttcggcc gcgaacagac agctacccct tcacgtcaag
     1801 caattcgacg tcgaacatca gcgtcgcatt cggcggaatg acgccgccag cgccgcgagc
     1861 gccgtagccg agcgccggcg ggatgatcaa ggtgcgcttg ccgccgacct tcatggtggc
     1921 gacgccttca tcccagccgg cgatcacctt gcgctgcccg atcggaaact cgaacggctc
     1981 attgcgatcc accgaactgt cgaacttctt gcccttcttg ccgccctcgt agagccaacc
     2041 cgtgtaatgc atgacgcaga tctggccggg ttttggcgtg gcgccggtgc cgacggtgct
     2101 gtcggtgatc tgcaagcctg atggtgtggt catggttttt cccgcggttt gggccaatac
     2161 gcttggcggc attgcgatga gaaggacgcc agcaacggca acggcgattg ggagatttcg
     2221 aaacatgtaa tgtcgcatct tttcaaattc cttcgtaaca gggacggaaa gcctcatgcg
     2281 cagacggttc gttcaatacc cgagcgcgca gccatccttg cgcggatcgg aaccgccggt
     2341 gagcgtgccc ttgtcccagt cgatccagat cgcctggccg ccgccgtgcg gcgtgacgac
     2401 gctcgcggtc cgatgcccga gcttcctcag gccatcgacg atatcggccg gcacgccatc
     2461 ctccagctgg tagacacctt cgtaatgcag gccgcgcggc atatcgatcg cctcctgcac
     2521 gtcgcagccg tagtcgagca cgttggtcag cacgtgcacc tgcccgaccg gctggtactg
     2581 cccgcccatc acgccgaacg gcatcaccgc ccggccggcc ttggtgacaa ggcttgggat
     2641 gatcgtgtgc agcggccgct tgcccggcgc gatgcaattg ggatggccgg gctgaacgcg
     2701 gaaaccgccg ccgcgatttt gcaggagaat gccggtcttg ttggagacga tcgccgaacc
     2761 gaacgcatgc gcgatcgaat tgatgaatga acagacgttg cggtcgcggt ccaccacggt
     2821 gacgtagacc gtcgaggggt tcatcggcgg cgacacattc ggcagatcga gcaaccggtc
     2881 catcctgatc ttgctgccat actcctcggc gaattccttc gtcaagatcc tgacgacatc
     2941 gacgtcgaca aaagcgggat cgccgatgta ctgctcgcgc atcatgtagg cgatgcgcgc
     3001 ggcttctgct tcgagatgga accgctcgac gctcatcggc ggaaatttcg tcagatcgaa
     3061 gcgcgacagg atgttcagca tcaccagcat
//
LOCUS       DASWGM010000634         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_338287,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000634 DASWGM010000000
VERSION     DASWGM010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338287"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VGA15_17910"
     CDS             <1..239
                     /locus_tag="VGA15_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRC-barrel domain containing protein"
                     /protein_id="HEX9589604.1"
                     /translation="PFDKIKFVNEPVAYTGVAGAPNAPGSRPASSTTTGAATTNTPPA
                     AATTPKSNPWYPDHAVFNATKDELKAMPEFKYST"
     gene            881..2125
                     /locus_tag="VGA15_17915"
     CDS             881..2125
                     /locus_tag="VGA15_17915"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX9589605.1"
                     /translation="MSAQRFLASRDVTHQEILVTAGQRTASACAGRRIVAAQDTTEIN
                     FKDRDRARKGLGPAGDGQTPGFFCHAMVAIDAEDEALLGVVHARIWTRSSEPVTARRN
                     RPIEQKESIRWIEATAVAGDRLGSAAQLIVLGDREFDIYEQFVRIPPGVELIVRAAQN
                     RKLADDDDKLLFAAASDWREFGTMEVRVPPHRPGELARIARVGVKAGRVCIAKPCNRA
                     NSADPASVTLTLVEVHEIDPPKGHNPIVWRLLTTMPVCGQPDEFAAANEIVQLYRLRW
                     RIEQTFRAMKSDGLRLEETQVQEASRLFKLTAIALIAAARTIQLVDARDGSSRPASDV
                     ADDDLITAAEAIGPTLEGKTERQKNPHPKRSLSWLSWIVARLGGWNCYYKPPGPKTVR
                     TGWTKLADRAEGYRLAMAMQNV"
BASE COUNT          506 a          727 c          795 g          477 t
ORIGIN      
        1 ttcccttcga caagatcaaa ttcgtcaacg agccggtcgc ttacaccggt gttgcgggcg
       61 cgccgaatgc gcccggcagc cggccggcgt catcgacgac cacgggtgct gcaaccacga
      121 acactccgcc ggccgcggcg acaacgccga agtccaatcc gtggtatccg gaccacgccg
      181 tgttcaacgc gaccaaggat gagctgaagg cgatgccgga gttcaagtat tcgacctgag
      241 gcaaagcctg aatagatctg tcaaatagaa gcgcgcccgg tttcaccggg cgcgcttttt
      301 ctgtttgctt gcctcgcccc gcttgcgaca gggcaatcgc atatggaatt tttctatgcg
      361 gtccgcacgc gaaccttgtt tgtttcgcgc gacatttcaa cgggtcgtcc ctgcgaacgc
      421 agggacccat agggcgatga ctctcgtttt ggcgctggag cagaggcctt ttttcacttt
      481 tggggccagg ggttatgggt ccctgcgttc gcagggacga cccgttgaga gcttgtgcga
      541 ggccactacc gcatcaacgc gcggctcaat tcagtcatat gcgattcccc tccctcgcaa
      601 ggctacggca tctcgcgatt tgagtcgggt gatcgacact ttttcggcct cctgtcaaga
      661 atccgagacc gaaaatttcg agattcttcg aaggtcgccg ctgtgattca gtgccgcctg
      721 gacgacattc agggggatga cgatgcggtg tgacgatggg tttcatatcg gctattttgg
      781 cgatgcacgg cgacagagag cgggagcggc gctgtgcgag cgggtggtgg agacgggatc
      841 cctggtgttg cgacacgtgg gaagcgatcg gcgtggagag atgtcggcac aacgttttct
      901 tgcgtcgaga gatgtgacgc atcaggaaat cctcgtcact gcgggacaac gaaccgcgtc
      961 cgcctgtgcg ggacggcgga tcgtggcggc gcaggatacc acggagatca atttcaagga
     1021 tcgtgatcgc gcgcgcaaag gactgggacc ggccggcgac ggtcagacgc cgggcttttt
     1081 ctgccatgcg atggtggcga tcgatgccga ggacgaggcc ttgctgggtg tcgtgcacgc
     1141 gcgcatctgg acccggtcgt ccgagccggt gacggcacgg cggaaccggc cgatcgagca
     1201 gaaggaatcc atacgctgga tcgaggcgac ggcggtcgcc ggcgatcggc tcggcagcgc
     1261 ggcacaactg atcgtgctcg gcgatcgtga gttcgacatc tacgagcagt tcgtccgtat
     1321 tccaccgggc gtggagttga tcgtgcgtgc cgcgcagaat cgcaagctcg ccgatgatga
     1381 cgacaaattg ctgtttgccg cggcctccga ttggcgcgag ttcggcacga tggaggttcg
     1441 tgttccgccg catcggccgg gcgaactcgc acgcatcgcg cgcgttggcg tgaaggcggg
     1501 gcgggtatgc atcgccaagc catgtaaccg agccaattcg gccgatccag catcggtgac
     1561 actcacgctg gtggaagtcc acgagatcga tccgcccaaa ggccacaatc cgatcgtctg
     1621 gcgtctgctc accacgatgc cggtttgcgg ccaaccggac gagttcgccg ccgccaacga
     1681 gatcgtgcaa ctgtatcggc tgaggtggcg catcgagcaa accttccgcg ccatgaaaag
     1741 cgacgggctg cgcctggagg agacgcaagt ccaggaagct tcgcgcctgt tcaagctcac
     1801 cgcgatcgcg ctgatagcgg ccgcgcgcac cattcaactg gtcgacgcca gagatggaag
     1861 cagcagacct gcatcggatg tcgccgacga tgatctcatc actgccgccg aagcgatcgg
     1921 cccgacactc gaaggcaaga ctgagcgcca gaagaatccg catcccaaac gctcgctgtc
     1981 ctggctgtcc tggatcgttg cccgtctcgg cggatggaat tgctactaca agccgcccgg
     2041 tccgaagacc gtgcgaaccg gatggaccaa acttgccgac agggcggaag gctatcgtct
     2101 tgcaatggct atgcaaaatg tgtgaatccc gtagcccctt ggggaggggt aatcgcatat
     2161 gaatgaattg agccgtgtgt tgatgcggta ttggcgtagc acaagctctc agcgggtcgt
     2221 ccctgcgaac gcagggaccc ataacccctg gcctcaaaag tgaaaaaagg cctctgctcc
     2281 agcgccaaaa cgagagtcct cgccgccgcg atgagcacaa ttgcgctcac gctggggtcc
     2341 ctgcgttcgc agggacgact cgttgaaatg tcacgcgaaa caaacaaggt tcgtgtacgg
     2401 accgcatgga aaattccata tgcgatagcc ctgcccgcaa gcgggagagg tggtgaaact
     2461 tactgcgcca gatgcgccgt caacggatga ttgccggctt gattg
//
LOCUS       DASWGM010000635         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2575850,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000635 DASWGM010000000
VERSION     DASWGM010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2575850"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGA15_17920"
     CDS             <1..592
                     /locus_tag="VGA15_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589606.1"
                     /translation="EPQEIAGFALEGSRHAGSLRGIASDVESRCVVAVHGEGRRISEL
                     GKDHVAQIGVNFALFRIGARHLDPVTIRRRRRPSDKMIVLICGDNEQRVIFGDPVLLR
                     AIEEFAEGLVVCGKLLDVPALAGAEGATTGDVIVVGVGDITNQSLRAPLTGVQARLNG
                     RVRGLARAGEVRGWPMEFSTFGSYRLQERLPLWRWR"
     gene            complement(787..1908)
                     /locus_tag="VGA15_17925"
     CDS             complement(787..1908)
                     /locus_tag="VGA15_17925"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9589607.1"
                     /translation="MADVFRRFAGGYEARYGAAVLPSHRRAIADIIACRTEALGADLW
                     CCPQCGTLVPVFHSCKNRHCPQCNGARTQAWLEQRQAEILPIPYFHVTITVPKELRAA
                     LRSHQTAGYGALMKAAAESIIKLARDPRWVGGTVGVLAVLHTWTQQLHLHPHVHCLVT
                     GGGLSDADRCWHSAEKKFLFPTKALGKLVRTQFRNVFNKLCPDISLPPHTWRRPWVVH
                     VTPWSEGKAALEYFARYAFRIAITDRRIIRVDDTGVTFRYKDRAADCHRTGTLSGEEF
                     VRRFLQHVLPQGFHKLRYYGLWHPARRHQLDNLRNALLLERPAPPSSSDTQPDEAAVG
                     ETDTADKPRMPTPLRLCTQCGQAYLVKLPRAFPASPLGP"
     gene            complement(1945..2520)
                     /locus_tag="VGA15_17930"
     CDS             complement(1945..2520)
                     /locus_tag="VGA15_17930"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9589608.1"
                     /translation="MRLPKQKRLPQALSHAEVQRILGNVRNPIHKACFALIYACGLRI
                     SEAATLEVTAIDKVNRLLRILGKGDKERLVPLPRPVHDGLREMWKAHRNPRWLFPNSW
                     GTGPLSTNVLVRTFADAAQAADLPAQRRATPHTLRHSYATRLFEKKVDTRVIQVLLGH
                     QSITTTAIYTHLTEPARASLHKLLDKLMSGL"
     gene            complement(2412..2789)
                     /locus_tag="VGA15_17935"
     CDS             complement(2412..2789)
                     /locus_tag="VGA15_17935"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9589609.1"
                     /translation="MTPLRARMIEDMKLGGLADKTQEVYQQAVRGLAKHYRRPPDQLS
                     EAEVRRYLLDVKEQGAARGTFKTCHYGIQFFYRQTLGRDWPLFKKRSDCPSRSGCRRP
                     SRMPRSSGSSATSAIRSTRPALP"
     gene            2685..3419
                     /locus_tag="VGA15_17940"
     CDS             2685..3419
                     /locus_tag="VGA15_17940"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9589610.1"
                     /translation="MLGQAAHRLLINLLCLVGQSTQLHVLDHTGAQRCHRLLLRRRHT
                     GGTTPPRHRRIASPASLRPCRHSLGRSRSVQLNSHHHLRDLSDRALIATLTYSFARIS
                     AALKMKVEDLRPRGAAWTVRLHVKGGKEHAMPCHHALAEALRAYIDAAGIAEDRKGWL
                     FRTARGHKADALSDRAMNQSDAWCMIRRRAKAAGIAAEIGCHTFRATGITAYLANGGA
                     ESRAKGIAHAKAAIEQLLHGVYKQAA"
     gene            3514..>3766
                     /locus_tag="VGA15_17945"
     CDS             3514..>3766
                     /locus_tag="VGA15_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9589611.1"
                     /translation="MRSETETLQWTTMARPREFDEADALDAATDCFWRDGYEATSVRD
                     LAARMGITGASLYNAFGDKRSLFREVLQRYAERSTRERIA"
BASE COUNT          662 a         1123 c         1295 g          686 t
ORIGIN      
        1 cgaaccccag gagatcgccg gcttcgcgct cgagggttcg cgtcacgctg gcagtctgcg
       61 cgggattgcc agtgatgtag aaagtcggtg cgtcgtcgct gtgcacggtg aagggcgtcg
      121 tatctccgaa ctcggtaaag atcacgttgc tcagatcggc gttaatttcg ccctttttcg
      181 cataggtgca aggcacctcg atcccgtcac aatccgccgg cgaaggcgcc cctccgacaa
      241 aatgatcgtt ctcatctgcg gtgataacga acagcgcgtt atctttggtg atcccgtcct
      301 tctgcgggcg atcgaagaat ttgccgaagg ccttgtcgta tgcggcaagc tgctggacgt
      361 acccgctctc gccggggccg aaggtgccac taccggtgat gtgatcgtcg tgggcgtcgg
      421 cgatataacg aatcagagtt tgcgcgcccc tcttacgggc gtgcaggctc ggttgaacgg
      481 acgcgtgagg ggtctcgctc gcgccggcga ggtgaggggc tggcctatgg agttttctac
      541 ttttgggagt tatcgactac aggagagatt gccactttgg cgatggcgat gagcggagca
      601 aacggcggca gggcatgttg tgggcagtgg ctcggccggg gctgctgacg cctggcggta
      661 gtcgatcggc gctacggtcg ccaggcggcg cgagggaagg cgacgaggcc aaagtgatcc
      721 ggcacccgcg ttgggggcgg gcttcggtca ggaccaggtg gatgaggtaa ggtgcgtgca
      781 ggtcggtcat ggtcccaggg ggctggcggg gaaggcacgc ggcagtttga cgaggtaggc
      841 ttgaccgcac tgggtacaca gccgtagcgg tgttggcatc cgcggtttgt cggcagtgtc
      901 ggtttcgccg accgcagcct catcgggctg ggtgtctgag gatgacggcg gggccggccg
      961 ttcgaggagc agagcattgc gcaggttgtc gagctggtgg cgccgggcag gatgccagag
     1021 gccatagtag cgcagcttat gaaatccctg cggcagaaca tgctggagaa agcggcgcac
     1081 gaactcctcg ccggagagag tgccggttcg gtggcagtcg gcggcccggt ccttgtagcg
     1141 gaaggtgacg ccggtatcgt cgacgcggat gatccgtcgg tcggtgatgg cgatgcggaa
     1201 ggcatagcgg gcgaagtatt cgagcgccgc ctttccctcg ctccagggcg tgacatgcac
     1261 gacccatggt ctgcgccagg tgtgcggcgg caggctgatg tccggacaca gcttgttgaa
     1321 cacgtttcgg aattgggtgc gaaccagttt gccgagggcc ttggttggaa acaggaactt
     1381 cttctcggcc gaatgccagc agcgatcggc atcggaaagg ccgccgccgg tgaccagaca
     1441 gtgcacatgt gggtgcaggt ggagctgttg cgtccaggtg tgcagcacgg ccaagacgcc
     1501 gacggtgccg ccgacccaac gcggatcgcg agccagtttg atgatggatt cggcagcggc
     1561 cttcatcagg gcgccgtagc cggctgtctg gtggctgcgc aaggctgccc gcagctcctt
     1621 ggggacggtg atggtgacgt ggaagtaggg gatgggtagg atctcggctt ggcgctgttc
     1681 gagccaagcc tgtgtccgcg cgccgttgca ttgtgggcaa tggcggttct tgcaggaatg
     1741 gaagacggga accagggtac cgcactgggg gcagcaccac aggtctgcgc caagcgcttc
     1801 ggttcggcag gcgatgatgt cggcgatggc gcgacggtgg gatggcaaca ccgccgcgcc
     1861 atatcgcgcc tcatagccgc cggcgaagcg tcggaaaacg tccgccaccc ccgccgcagc
     1921 gggagtggca ccgacgggca cgcgtcagag gccgctcatc aatttatcga gcagcttgtg
     1981 caaggaggcc cgcgccggtt ccgtcaggtg ggtataaatc gccgtcgtgg tgatgctctg
     2041 gtggccgaga agcacctgga tgacgcgggt gtcgaccttc ttctcgaaga gcctggtggc
     2101 ataggaatgc cgcagggtat ggggtgtcgc ccggcgctga gcgggcaggt cggccgcctg
     2161 agcggcatcg gcaaaggtcc tgaccaggac gttggtgctg agcgggccgg ttccccagct
     2221 gtttggaaag agccagcgcg gattgcggtg cgccttccac atctcgcgca aaccgtcatg
     2281 gaccggcctc ggcagcggca cgaggcgttc cttgtcgccc ttgccgagga tccgcaagag
     2341 ccggttgact ttgtcgatgg cggtgacttc gagtgtggca gcctcgctga tccgcaggcc
     2401 gcaggcatag atcagggcaa agcaggcctt gtggatcgga ttgcggacgt tgccgaggat
     2461 ccgctggacc tcggcatgcg agagggcctg cggcagccgc ttctgcttgg gcagtcggat
     2521 ctttttttaa acagcggcca gtcgcggccc agcgtctgcc ggtaaaagaa ctggatccca
     2581 taatggcagg tcttgaaggt tccccgagcc gctccctgct ccttgacatc cagcagatac
     2641 cggcggacct ccgcctcgct caattgatcc ggcggccgtc ggtaatgctt ggccaagccg
     2701 cgcaccgctt gctgataaac ctcctgtgtc ttgtcggcca atccacccag cttcatgtcc
     2761 tcgatcatac gggcgcgcaa cggtgtcatc gtctgctcct tcgtcggcgg cacacaggtg
     2821 ggacaacccc acctcggcat cgaaggatag cttcaccggc aagcctcagg ccatgccgcc
     2881 actccctagg gcgtagccgt tccgttcaac tcaattccca ccaccacctg cgcgatctga
     2941 gcgaccgcgc cttgatcgcc accctcacct acagcttcgc gcgcatctcc gcggctttga
     3001 agatgaaggt cgaggatctc cggccgcgcg gcgcggcatg gaccgtccgg ctacacgtga
     3061 aaggcggcaa ggagcatgcg atgccgtgcc atcacgcgct cgccgaggcc ttgcgcgcct
     3121 acatcgacgc cgccggcatc gccgaagacc gcaagggctg gctgttccgc accgcgcgcg
     3181 gccacaaagc cgatgcgctg tccgaccgcg ccatgaacca atccgacgcc tggtgcatga
     3241 tccgccgccg cgccaaggcg gccggcatcg cggccgaaat cggttgccac acgttccgcg
     3301 cgaccgggat caccgcctac ctcgcgaacg gcggtgccga gagccgggcg aaggggattg
     3361 cgcacgcaaa ggccgctatc gaacagttgc tccacggcgt ctacaagcag gcggcgtgag
     3421 ccgccgccgg cgttgcttgc catggcatcc ccacaaaacc gcatggcagc tttgtccaac
     3481 ttttttatag cgatcgatct aaaacgagcc aagatgcgct cggaaaccga aacgttgcaa
     3541 tggacgacga tggcaaggcc gcgggagttt gatgaggcag acgcgctcga cgcggcgacg
     3601 gattgcttct ggcgggacgg ctacgaagcg acctcggtgc gggatcttgc cgcccgcatg
     3661 ggcatcaccg gcgccagcct ctacaacgcg ttcggcgaca agcgttcgct gtttcgcgag
     3721 gtgctgcagc gctatgccga acgatcgacg cgcgagcgca tcgccc
//
LOCUS       DASWGM010000636         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_338536,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000636 DASWGM010000000
VERSION     DASWGM010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338536"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..488
                     /locus_tag="VGA15_17950"
     CDS             78..488
                     /locus_tag="VGA15_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 family protein"
                     /protein_id="HEX9589612.1"
                     /translation="MQFDTKIAVVIRTDLEPWQKLNVASFLTSGIAAAFPECIGEPYA
                     DGSGTQYLSLIGQPILIYGADRPALNRALERALAREVKPAVYTEDMFKTTHDAANRDA
                     VKAVARADLNLVGLAMRAERKVIDKIVDGLKFHS"
     gene            723..2069
                     /locus_tag="VGA15_17955"
     CDS             723..2069
                     /locus_tag="VGA15_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX9589613.1"
                     /translation="MADEQLFEDLPEQERPQAEVRGALRLRVPERSQIGMQVAALDDL
                     ISEDHPVRSVWAFVEGLDLSALHEVIKAREGQPGHPPAAPELMVALWLWATVDGVGGA
                     RQLDRLCRDHLVYRWLCGGVSMNYHSLSTFRTAHMAVLERLLAQGVASMVQAGLVSLD
                     TLAQDGLRVRAGAGAGSFRRHARLDDLLTAAQTRVKRLRAEVETDPATGDRRQRAAKE
                     RGAREREARIAAAQQRMKELEAERERREKTNKKEVAKQKEPRASTTDPDARVMKMADG
                     GWRPAYNMQIISTPEHQVIVAVDIDTSGSDRGLARPGIETVHAEGYEPSNYLVDGGFT
                     KHDDIEWAHAKDIKMWCPPGQTRHGTDPYAPKRDDGVGVADWRERMKGEEDKKSEALY
                     RLRAEHECINAHARRMGLRQLSLRGKIKARIHLLWFALARNMMRFFALGAKALAAA"
     gene            complement(2463..>3142)
                     /locus_tag="VGA15_17960"
     CDS             complement(2463..>3142)
                     /locus_tag="VGA15_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9589614.1"
                     /translation="RNYMRHMTGSVYAAFEKSVHMYRGTTMAGIIRDEAANDPKRGFV
                     GGYEMETLSLGLPFMAAFLKPGAWGRSFTSAMESYPRMAGMWLVGEDMAQETNRITLD
                     PVAKDKFGMPVASVHFDDHPNDVAMRAHAYKQGSAVYEAVGATVTYPTPPYPSTHNLG
                     TNRMSEKPRDGVVNKFGQTHDIKNLFVSDGSQFTSGAACNPTLTIVSLAIRQADTIAS
                     AMQKKEI"
BASE COUNT          595 a          919 c         1052 g          576 t
ORIGIN      
        1 tcgagacggc gcttacgcgc ctcctcacca tgaggaaccc ggttctctca acccctcaaa
       61 tgcgtcggta atctgcaatg caattcgata ccaagatcgc ggtcgtgatc cggaccgacc
      121 ttgaaccgtg gcagaagctg aacgtcgctt cgttcctgac cagcggcatt gccgccgcgt
      181 tcccggaatg catcggcgaa ccctatgcag atggttcagg cactcaatat ttgtcgctga
      241 ttggccaacc gatcctgatc tatggtgccg accggccggc gctgaaccgc gcgctcgagc
      301 gggcgctggc gcgagaggtc aagcccgcgg tctataccga ggacatgttc aagacgacgc
      361 acgacgccgc caaccgggac gcggtgaaag ccgtggctcg cgccgatctc aatctggtag
      421 gcctcgccat gcgcgccgag cgcaaggtga tcgacaagat cgtcgacggg ctgaagtttc
      481 acagctagct agcgcatgaa ccggaaaagt ggttaccggt tttccgaaaa gatcatgctc
      541 aaacaaaaag atagagtggg atgacgattc gaagaaaagt catcgcgctc taggacggtg
      601 gccttatcct tcgagacggc gctttgcgcc tcctcagagt gtgagaatct ttcggtaacg
      661 agcgtttgcc acggccgcca tgctgtgatt cgctggcatt gagcgcgaag caggggctgg
      721 ggatggctga cgagcaattg ttcgaggatt tgcccgagca ggagagaccg caggcggagg
      781 tgcgcggtgc gctgcggctg cgggtgccag aacgcagtca gatcggtatg caggtggcag
      841 cattggacga tctgatttcg gaggatcatc cggtacgctc ggtttgggcg ttcgtcgagg
      901 ggctggatct gtcggcgttg cacgaggtca tcaaggcgcg cgagggccag cccggacatc
      961 cgccagcagc gccggagctg atggtggcgc tttggctatg ggccacggtc gacggggtcg
     1021 gcggcgcccg ccaattggac cggctgtgcc gcgatcatct ggtctatcgc tggttatgcg
     1081 gcggggtgtc gatgaactat cacagcctgt cgaccttccg gaccgcccat atggcagttc
     1141 tggagcggct actggcgcag ggcgtcgcgt cgatggtgca ggctgggttg gtgtcgctcg
     1201 acacgttggc gcaggatggc ctgcgggtac gcgcgggtgc cggggcgggt tcgttccgcc
     1261 ggcacgcgcg gcttgatgat ctgctcaccg cggcgcagac gcgggtgaag cgcctgcgtg
     1321 ctgaagtcga gacggatccg gcgaccggcg atcgtcgcca gcgcgcggca aaggagcgtg
     1381 gtgcccgcga gcgcgaggcg cggatcgcag cggcacagca gcgcatgaag gaactggagg
     1441 ccgagcggga acggcgagag aagaccaaca agaaggaggt ggccaagcag aaggagccgc
     1501 gcgcctcaac caccgatccc gacgcgcggg tgatgaagat ggcggatggt ggctggcgcc
     1561 ccgcttacaa catgcagatc atatcgacgc cggaacatca ggtgatcgtt gcggtcgaca
     1621 tcgacaccag cggttcggat cgcggcctgg cacgaccggg gatcgagacc gtgcatgctg
     1681 aaggctatga gccctccaac tatctggtcg atggtggctt cactaaacac gacgacatcg
     1741 aatgggcgca tgccaaggat atcaagatgt ggtgcccgcc ggggcagacc aggcatggca
     1801 ctgaccccta tgcgccgaag agagatgacg gcgtcggcgt cgccgattgg cgtgaacgca
     1861 tgaagggcga ggaagacaag aaaagcgagg cgctctaccg actgcgcgcc gagcacgaat
     1921 gcatcaatgc ccatgcccgc cgcatgggcc tgcgccagct atcgctgcgc ggcaagatca
     1981 aggcccgcat ccatctgctg tggtttgcgc tcgctcgcaa catgatgcgc ttcttcgcgt
     2041 tgggcgccaa ggcgctggca gcggcatagc agggagccca cgctgacagc acctacaaag
     2101 cgccctcgtc tccgggtcgg ctcagccagg aattggggta gcccagcttc cgcaatccgc
     2161 ggaattgggc ttgatcgccg ccattcagtc gccggcccgg ccgaaaactc ctcggaaggt
     2221 gttcttcagg ccagcagccg taaaattttg ctggcaagag cgaaacattc tcaccctctc
     2281 aggacgaggg gctagaccct catggtgagg agcgcggtaa caccgcgcgt ctcgaaccat
     2341 gaggcctcaa ccgcatgatt atgattctgt ccaagcaaaa atggcctagc gtccaagacc
     2401 cgcgactaga tcggaaatgg ctccgtcccc tcctggacgg agccgctgta ttctgagcgc
     2461 ggtcagatct ctttcttctg catcgcgctt gcgatggtgt cggcctgccg gatcgccagc
     2521 gagacgatgg tcagcgtcgg gttgcaggcc gcgccgctgg tgaactggct gccgtcggac
     2581 acgaacaggt tcttgatgtc gtgggtctgg ccgaacttgt tcaccacgcc gtcccttggc
     2641 ttctcgctca tccggttggt gccgagatta tgtgtcgagg gatagggcgg ggtcggatag
     2701 gtcacggtgg cgccgaccgc ctcatagacc gctgagccct gcttgtaggc gtgggcgcgc
     2761 atcgcgacgt cgttcggatg atcgtcgaaa tgcacgcttg cgactggcat gccgaacttg
     2821 tccttggcga cgggatcgag cgtgatgcgg ttggtttcct gcgccatgtc ctcgccgacc
     2881 agccacatgc cggccatccg cggataggac tccatcgcgc tggtgaagga gcggccccag
     2941 gcgcctggct tgaggaacgc ggccatgaag ggcaggccga gcgacagcgt ttccatctcg
     3001 tagccgccga cgaagccgcg ctttggatcg ttggcggctt cgtcgcggat gatgccggcc
     3061 atggtggtgc cgcggtacat gtgcaccgat ttttcgaatg cggcatacac gctgcccgtc
     3121 atgtgccgca tgtagttgcg gc
//
LOCUS       DASWGM010000637         3905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_603735,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000637 DASWGM010000000
VERSION     DASWGM010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603735"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /gene="ruvA"
                     /locus_tag="VGA15_17965"
     CDS             complement(<1..282)
                     /gene="ruvA"
                     /locus_tag="VGA15_17965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX9589615.1"
                     /translation="MIGKLKGLIDSYGEDFVILDVGGVGYQVHCSSRTLQALPAPGEA
                     AVLSIETYVREDQIKLFGFRSDTEREWFRLLQTVQGVGAKVALAVLSTLP"
     gene            complement(511..1038)
                     /gene="ruvC"
                     /locus_tag="VGA15_17970"
     CDS             complement(511..1038)
                     /gene="ruvC"
                     /locus_tag="VGA15_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX9589616.1"
                     /translation="MTYQPIRPAVRILGIDPGLRRTGWGVIEIDGNRLSFVGCGSVEP
                     PDGLPLASRLLAIHEGLATVLGDFRPAEAAVEQTFVNRDGVATLKLGQARGVAMLAPA
                     MFGISVAEYAPNQVKKTVVGAGHADKNQILVMLKILLPKAEPKSADAADALAIAITHA
                     HHRQSAALRLKVVGL"
     gene            complement(1283..2029)
                     /locus_tag="VGA15_17975"
     CDS             complement(1283..2029)
                     /locus_tag="VGA15_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9589617.1"
                     /translation="MAGHSQFKNIMHRKGRQDAQKSKLFSKLAREITVAAKMGQPDPS
                     MNARLRAAITSARQENMSKDSIERAVKKAIGGDAENYDEIRYEGYGPGGVAVIVEALT
                     DNRNRAASDIRSYFTKSGGNLGETGSVAFMFDHTGIIEYDAKVASDDAMLDAAIEAGA
                     DDVLSSENGHEIYASQETFRDVAKALEAKFGEARKAALTWKPQNTMPVDDETGEKLLK
                     LIDLLNEHDDVQNVYANFEVSDALMTKMGG"
     gene            2230..>3905
                     /locus_tag="VGA15_17980"
     CDS             2230..>3905
                     /locus_tag="VGA15_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9589618.1"
                     /translation="MAFGLFRKRLPEPAAPTAKPVAATPTIAAEIAGVSDSDSAKEIL
                     ELLELELGAMIRQLERAAHSVADGAEATAATLSTIRQRTNALTGHTSAAQGTATTFSQ
                     AADNFTHSAQGIGSQVRDASKLADEASAAAEEASLNVGRLRESSAAIGNVVNLIAQIA
                     RQTTLLALNSTIEAARAGDAGRGFAVVATEVKALAVQTQHATEEITKRIDALQRDAAG
                     SVDAVQRISLAIAAIRPVFDNVNGAVAEQNETTGKMSDNAASASQFIVSVGDSAAEID
                     SATREAEAHGKNVAEAGSAVTAFTQKLKSRCAVLLRQGEREDRRKRARLPCHLKIEIQ
                     TARGPVTAPVYEISIEGILIGGPDAERLPLNENLNAALENVGACRIRLSEHSKAGAQA
                     RFERPDAALVENIEDTLWALHDENIEFVTRAMEAGTALTKIFEDALASGAISVDDMFD
                     ADYVEISGTNPLQHRTRILDWADRALPPFQEAFLVRDPRMAFCVMIDRNGYLPVHNRI
                     YSHPQRSGDVVWNTANSRNRRIFNDPAGLAAGRNLRSYLIQSYARDMGNG"
BASE COUNT          737 a         1271 c         1214 g          683 t
ORIGIN      
        1 cggcaaggtg gagagcaccg ccagcgccac tttggcgccg acgccctgca cggtctgcag
       61 cagccgaaac cattcgcgct cggtgtcgct gcgaaagccg aacagtttta tctggtcctc
      121 gcgcacataa gtttcgatcg acagcaccgc cgcctcgccg ggcgcgggca gcgcctgcaa
      181 cgttcgcgac gagcaatgca cctgatagcc gacgccgccg acatcgagaa tgacaaaatc
      241 ctcgccgtaa gagtcgatca gacctttgag tttaccgatc atgattctgc tcttcccctc
      301 tccccttgtg gaagagggtg acgcaatgcg aacgcagtga gcattgcgtc gggtgagggg
      361 taagccgcag aacacagcgc ttggggttac ccccctcccc gaccctcccc caacgcaagt
      421 cgggcctgcc agacttgcgt aacaaaaaaa tgtgcaaccg gggtagactc cggttgctgg
      481 ggggagggag cgcattgtgc ttgcggaact tcataacccc accactttca gccgcagcgc
      541 cgcgctctgg cggtgatggg cgtgggtgat ggcgatggcg agcgcatcgg cggcatcggc
      601 ggatttcggc tcggccttgg gcagcaggat tttcagcatc accagaatct ggttcttgtc
      661 ggcatgtccg gcgccgacca cggtcttctt cacctgattg ggcgcgtatt cggcgaccga
      721 aatcccgaac atcgccggcg ccagcatcgc aacgccgcgc gcctggccca gcttcagcgt
      781 cgcgacgccg tccctgttga caaaagtctg ttcgaccgcg gcttcagcgg gacggaagtc
      841 gccgagcacg gtggcgagac cctcgtgaat cgccaacagg cggctcgcca gcggcaggcc
      901 atccggcggt tccaccgagc cgcaaccgac gaaactcagc cggttgccgt cgatctcgat
      961 caccccccag ccggtgcggc gcagccccgg atcgatgcca aggatgcgga cggcagggcg
     1021 aatcggctga taggtcatgc ccaagtgtta acgccggagc acggcgaacg aaatagaaac
     1081 ggggcgtcat accccgcgaa agcgggcgga gcgtgccccg gacgctgcgc agcaccataa
     1141 gcgcgtttac gcgcgtcttc gacgcgctat ggtgatgcgc tgcagagccg gggcccatgc
     1201 aatgcgttcc gcgtgtgatg ggtcccggct ctgcggagca ccgccgaaga ggcgctgcac
     1261 cgcgtccggg acacgaagca aactacccgc ccatcttcgt catcagcgcg tcggaaacct
     1321 cgaaattcgc atacacgttt tgaacgtcgt cgtgctcgtt caacagatcg atcagcttca
     1381 acagcttctc gccggtctcg tcgtcgaccg gcatcgtgtt ctgcggcttc caggtcagcg
     1441 ccgccttgcg ggcttcgccg aatttcgcct ccagcgcctt ggcgacgtcg cgaaacgtct
     1501 cctgcgaggc gtagatctcg tggccgttct cgctcgacaa tacgtcgtcg gcgccggcct
     1561 cgatcgccgc atccagcatc gcatcgtcgg aggcgacctt ggcgtcgtat tcgatgatgc
     1621 cggtgtggtc gaacatgaag gcaaccgaac cggtttcacc gagattgccg ccggatttgg
     1681 tgaagtagga tcggatatcg gacgccgcgc ggttgcggtt gtcggtcagc gcctcgacga
     1741 tgacggcgac gccgcccgga ccataaccct cgtagcgaat ctcatcgtag ttttcggcgt
     1801 cgccgccaat cgccttcttc accgcacgct cgatcgagtc tttcgacatg ttctcctggc
     1861 gcgcggaggt gatcgcggcg cgcagccgcg cattcatgct ggggtccggc tggcccatct
     1921 tggccgcaac cgtgatttcc cgcgccagtt tgctgaacag tttcgacttc tgggcatcct
     1981 gccggccctt gcggtgcatg atgttcttga actgggaatg tccggccatg cgggtgcctc
     2041 tgggattcgt gcgggatgtg ggggtggaag gcgcggcgtt ataggccggt aaccggccaa
     2101 aatcaaagat ttgcgggggt tttcggtata caggtagcgt ccgttgccta tctatcgggc
     2161 aattattaac catgatttca cgcccgactg cgagcatgct gtttcacttg cctgttttga
     2221 gattagccaa tggcgttcgg attgtttcga aagcgtctgc cggaaccagc cgcgccgacc
     2281 gccaaacccg tggcggcgac gccgacgatc gcggctgaaa ttgccggcgt ttcggacagc
     2341 gattccgcca aggaaatcct cgaactgctc gaactcgaac tcggcgccat gatcaggcag
     2401 ctcgaacgcg cggcccattc ggtcgctgat ggcgccgagg cgacggcggc gacgctctcc
     2461 accattcgac agcgcaccaa cgcgctgacc ggacacacca gcgccgcgca gggcactgcg
     2521 accacctttt cgcaggccgc ggacaacttc acccattccg cgcaaggcat cgggtcccag
     2581 gtgcgcgacg ccagcaagct cgccgacgag gccagcgccg ccgccgagga ggccagcctc
     2641 aatgtcgggc gcttgcgcga atcatcggct gccatcggca atgtcgtcaa tctgatcgca
     2701 cagatcgcca ggcaaaccac gctcctggcg ctcaactcca ccatcgaggc ggcgcgggcg
     2761 ggcgatgccg ggcgcggctt tgccgtggtc gccaccgagg tcaaggcgct ggcggtgcag
     2821 acccagcacg ccaccgagga gattaccaaa aggatcgacg cgctgcagcg ggacgcggcg
     2881 ggctcggtcg atgccgtgca acggatttcg ctggcgatcg cggcgatccg cccggttttc
     2941 gacaacgtca acggcgcggt ggcggagcag aacgaaacca ccggcaagat gtccgacaac
     3001 gccgcctccg catcgcagtt catcgtctcg gtcggcgaca gcgccgccga aatcgacagt
     3061 gcgaccaggg aggccgaggc tcatggcaaa aatgtcgccg aggccggcag cgccgtcacc
     3121 gcgtttacac agaaactcaa atcgcgctgc gcggtgctgc tgcggcaggg cgagcgcgaa
     3181 gaccggcgca agcgcgcgcg gctgccgtgc catctcaaga tcgaaattca gaccgcacgc
     3241 ggccctgtca cggcaccggt ctatgagatt tccatcgaag gcatcctgat cggcggaccg
     3301 gacgccgagc ggctgccgct gaacgagaac ctcaatgccg cgctggagaa cgtcggcgcc
     3361 tgcaggattc gcctcagcga gcattcaaag gccggcgcgc aggcgcggtt cgagaggccg
     3421 gacgccgcat tggttgaaaa tatcgaagac acattgtggg cgctccacga cgagaacatc
     3481 gaattcgtca cccgcgccat ggaagccggc accgcgctga ccaaaatatt cgaggacgcg
     3541 ctcgccagcg gcgcgatctc cgtcgacgac atgttcgatg ccgattatgt cgagatatca
     3601 ggcaccaatc cgctgcagca ccgcacccgg attctcgatt gggcggatcg cgcgctgcct
     3661 ccgttccagg aggccttcct ggttagggat ccgcggatgg cgttctgcgt gatgatcgac
     3721 cgcaacggtt acctgccggt gcacaacagg atctactcgc atccgcaacg atcaggcgat
     3781 gtggtgtgga acaccgccaa cagccgcaac cgccgcatct tcaacgatcc cgcggggctc
     3841 gccgccggcc gcaacctgcg ttcctatctg atccagagct acgcccgcga catgggcaac
     3901 ggcaa
//
LOCUS       DASWGM010000638         2500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2017984,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000638 DASWGM010000000
VERSION     DASWGM010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017984"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VGA15_17985"
     CDS             <1..320
                     /locus_tag="VGA15_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9589619.1"
                     /translation="TGTPLRDFLKIVGADMRAKYCWFQCADSDGYNSPLDMASALHPQ
                     TQMTFRFGDEILPRAYGYPMKIRVPTKLGFKNPKYVISMEVTNDYKGGYWEDQGYNSF
                     SGS"
     gene            503..1786
                     /locus_tag="VGA15_17990"
     CDS             503..1786
                     /locus_tag="VGA15_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX9589620.1"
                     /translation="MNLRSGGKTETRFAAYVEGLASVVGHVDRAGPLRDYCTGLMLPG
                     ERKSVEPMAAKTAPARTAAQHQSLLHFVGVASWSDEKVLAKVREMVLPSMTRHGPIEA
                     WLIDDTAFPKQGTHSVGVQHQYCGQLGKEANCQVAVSLSIANHSASLPVAYRLYLPKE
                     WAEDDARRDKAGVPLDIGFKTKPEIALDQIRWACKAGLPRGVALMDAAYGTDSRLRAG
                     MTALGVTYAVGIQPKILVWPPGAGPKRRGKPLNNTGRRDEPDLISVKQLALDLPKHAW
                     RTIRWREGSAEWLSSRFARVRVRVGHSQLMPELLSQEWLLVEWPEGEAAPTKYWLSTL
                     PANISFRQLVDFAKLRWHVERDYQELKQEVGLGHYEGRGWRGFHHHATMCIAAYGFLI
                     SEREMIPPSRPRSTTIFPTPVLPEGYRPRGSSIAA"
     gene            complement(1961..>2500)
                     /locus_tag="VGA15_17995"
     CDS             complement(1961..>2500)
                     /locus_tag="VGA15_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX9589621.1"
                     /translation="FGAGYIAYMTFMIAYVRDAGGGAAAQSVFWCLIGVSAFVTPWVW
                     RGVLARNSGGVSTAIILGVNALGAALPLFGHSALLLAISALVFGVAFFAVVASTTAFV
                     RFNYPLAEWPTGIAAMTIAFGIGQTLGPIAVGAVTDAFGSLSYALNVSAAMLALGAIA
                     AAFQRRLVHKSNAVIPGRE"
BASE COUNT          513 a          740 c          795 g          452 t
ORIGIN      
        1 ggaccggcac gccgttgcgc gatttcctca aaatagtcgg tgccgatatg agggcaaaat
       61 attgctggtt ccagtgcgcc gattccgacg gctacaattc accgctcgac atggcgtcgg
      121 cgttgcatcc gcagacccag atgacgttca ggttcggtga cgaaatcctg ccccgcgcct
      181 atggctatcc gatgaagatc agggtgccga cgaaactcgg gttcaagaac ccgaaatacg
      241 tgatctcgat ggaagtcacc aacgactaca agggcggcta ttgggaagac cagggctaca
      301 attcgttcag cgggagttga tctgctcaaa gcgcaactgc gtagggtggg caaaggcgtg
      361 cttgcgccgt gcccaccgac atggaagatg tttatggatt ggtgggctcg cttcgcttag
      421 tcttactgag tcataacgcc gcggggcggg actttcgaga atcaactact tgagaagatt
      481 cacgattagc gcgggggctg acatgaatct gaggagcgga gggaagaccg aaacccgatt
      541 tgcggcgtat gtggaggggc ttgcaagtgt tgtcgggcac gtggaccggg ctggcccgct
      601 gcgcgactat tgcaccggcc tgatgctgcc aggcgagcgc aagagtgtgg agccgatggc
      661 cgcaaagacg gctccggcgc ggacggcggc gcagcaccag tcgttgctgc attttgtcgg
      721 tgtcgcgtcg tggtcagatg agaaggtatt ggccaaggtg cgtgagatgg tgctgccttc
      781 gatgacccgg catgggccga tcgaggcctg gctcatcgat gacacggcgt tcccgaaaca
      841 ggggacgcat tcggtaggcg tgcagcatca gtattgtgga cagctcggca aggaggccaa
      901 ttgtcaggtg gccgtgtcgc tctcgatcgc aaaccactcc gccagcctgc cggtagctta
      961 ccggttatat ctgccgaagg agtgggccga ggatgatgcg cgtcgcgaca aggcaggcgt
     1021 gccgctggat atcggcttca agaccaagcc ggagatcgcg ctcgatcaaa tccggtgggc
     1081 gtgcaaggcc ggccttccac gtggggtcgc attgatggac gccgcctatg gcactgactc
     1141 gcggctgcgt gctggcatga cggcgttggg agtgacctat gccgtcggca ttcagccgaa
     1201 aattctggta tggccacccg gcgccggacc gaagcgtaga ggcaagccgc tgaacaatac
     1261 gggccgtcgc gacgaacccg atctgatctc ggtcaaacag ctggcgcttg atctgcccaa
     1321 gcatgcctgg cgcacgatcc gatggcggga aggttcggcc gaatggctgt cgtcgcgctt
     1381 tgcccgcgtg cgcgttcggg ttgggcatag ccaactgatg ccggaattgc tgtcgcagga
     1441 gtggttgctg gtcgagtggc ccgagggcga agccgcgccg actaagtatt ggttatcgac
     1501 gctgccggcg aatatcagct tccgccagct cgtcgacttt gccaagctgc gctggcatgt
     1561 cgagcgagac tatcaggaac tcaagcagga ggtcgggctc ggacactatg aagggcgcgg
     1621 gtggcgcggc ttccatcatc acgccacgat gtgcatcgca gcttacggat tcctgatctc
     1681 cgagagggag atgattcccc cctcaagacc tcgttccact acgatcttcc cgacgcctgt
     1741 ccttcccgaa ggctatagac caaggggatc ctccattgcg gcctgaacgc cacatcccga
     1801 actcgatcgc aaccatgcgc gtccgcttga tcaacgcgat cataagaacc ttgccgcgat
     1861 gtccttgctg cggcgcaaat gctgcgagac acaggcgacg aaaaattatg acgcagtaag
     1921 attagcccac cctacagcct ggattccgcg ctcgctcgtt tcactcgcgc cccggaatga
     1981 cggcatttga cttgtgcacc agcctcctct gaaaggccgc ggcaatggcg ccgagcgcca
     2041 gcatcgccgc cgagacattg agcgcatagg agaggctgcc aaacgcgtcg gtaacggcgc
     2101 cgaccgcgat cggccccagc gtctggccga ttccaaacgc gatcgtcatc gccgcaattc
     2161 ctgtcggcca ttccgcgagg ggatagttga agcgcacgaa ggcggtggtc gatgccacca
     2221 cggcgaaaaa cgcgaccccg aacaccagcg ccgatatcgc cagcaacagc gcggaatgcc
     2281 cgaacagcgg cagcgcggcg cctaaagcgt tgacgccgag aatgattgcc gtggagacgc
     2341 cgccgctgtt gcgcgccagc accccgcgcc acacccatgg cgtcacgaac gcgctcacgc
     2401 cgatcaggca ccagaacacg ctctgcgccg cggcgccgcc gccggcatcg cgcacatagg
     2461 caatcatgaa ggtcatgtag gcgatgtagc cggcgccgaa
//
LOCUS       DASWGM010000639         1972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_468287,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000639 DASWGM010000000
VERSION     DASWGM010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468287"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VGA15_18000"
     CDS             <1..892
                     /locus_tag="VGA15_18000"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX9589622.1"
                     /translation="HLVPVGTHDRLLFFTSAGKVYEKKAYELPEGGRAARGRSIANLL
                     SLAGDETLSAFLPVPKDTAGKYVFFATRRGRVKKTELDEFTNIRSSGIIAINLEDGDE
                     LIGVRITDGNQQIVLSTREGQAIRFKEEEARSMGRAAGGVIGMELESHTVKEGKTVTL
                     VQDEVVSIAVARDDETLLTASEYGYGKRTPAAEYRLTHRGGKGVITMDVTERTGKVVG
                     VLQVGTDDQVVLITDHGKVIRLKAKQVRITGRNAQGVHMVKLEAAEKLRAIAPLAERE
                     DDEDGNGGNGADSNGGGGEK"
     gene            1158..>1972
                     /locus_tag="VGA15_18005"
     CDS             1158..>1972
                     /locus_tag="VGA15_18005"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9589623.1"
                     /translation="MPLDTSLVGVTAGPVASEIDARWTMAYAAGIDDTAACYLDTRRL
                     QGIIAHPLFPVCFEWPAALAIRAKSRGSLAQAEASRGVHATQHMILHRPVRPPMRLTT
                     IATLIGMEARKPGAYEVTKFETVDDKGTPVCTTYSGSIYRQVEIRGAERPAAMPKPPK
                     LAKLPAKPRAEIKVAIPAGAAHVYTECARIWNPVHTDAAVAAAAGLPAIILHGTATLA
                     MAVSRIVMAEAGGDPERIVEIYGRFGAMVLMPSEVMVRIHAREKASGGEAVLF"
BASE COUNT          400 a          570 c          668 g          334 t
ORIGIN      
        1 gcatctcgtg ccggttggaa cgcatgaccg gctgctgttt ttcaccagcg ccggcaaggt
       61 ctacgagaaa aaggcgtacg agctaccgga aggcggacgc gcagcgcggg gacggtcgat
      121 tgcaaacctg ttgagcctgg cgggcgacga aacactctcg gcttttctcc ccgtgccaaa
      181 ggacaccgcg ggcaaatatg tctttttcgc gacgcggcgc ggaagagtga agaaaaccga
      241 actcgacgag ttcaccaata tccgctcgag cggaattatc gcgataaatc tcgaggatgg
      301 cgacgaatta atcggcgtgc gcatcaccga cggcaatcag cagatcgtgc tgtcgacgcg
      361 cgaagggcag gcgatccgtt tcaaggagga ggaagcgcgc tcgatgggtc gtgcggcggg
      421 gggcgtgatc ggaatggaac tcgagtctca caccgtcaag gaaggtaaga cagttacgct
      481 ggttcaggac gaggtcgtgt cgattgcggt cgcgcgcgac gacgagactc tgctgacagc
      541 ctcggagtat ggctacggca agcgtacgcc cgcggcggaa taccgcctga cgcatcgcgg
      601 tggcaagggc gtgatcacga tggatgtgac ggaacgaacc ggcaaggtcg tgggcgtgct
      661 gcaggtcggt accgacgacc aggtcgtgct aatcaccgac catggcaagg tgatccggct
      721 caaggccaag caggtgcgca tcacggggcg caacgcccag ggcgtgcaca tggtgaagct
      781 ggaggcggca gaaaagctcc gcgccatcgc cccgctggcg gagcgtgagg atgacgagga
      841 cggcaacggc ggcaatggtg ctgactcaaa cggcggcggc ggcgagaagt agtgtctgat
      901 cagcaaccta acagattacc gtcactccca acggccgatg acttgtcatt ctacgcgcgt
      961 ttggcgcgaa gaatcccggg tgttcgatct tcagagcgcc gggattctcc gcgaggcacg
     1021 cggagaatgt cgaaatgggg ctctgctcgg gggtaagaaa atcgctccgc gtcgatcact
     1081 cgacgggcat ccacgtaagc tgctcttccg aatcggcgcg ttggacctct ctatgcgcgt
     1141 ggcgctaaaa ataggccatg ccactcgata catcgttggt tggagttacg gcggggccgg
     1201 ttgcaagtga aatcgacgcg cgctggacca tggcgtacgc cgccggtatc gacgacaccg
     1261 ccgcatgcta tctcgatacg cgtcgcctgc aggggatcat tgcgcatccg ctgttcccgg
     1321 tatgcttcga atggccggcg gcgctcgcca tccgcgccaa gtcacgcggc tcgctggcgc
     1381 aggccgaggc gtcgcgcggt gtccatgcca cgcagcacat gatcctccat cgcccggtgc
     1441 ggccgccgat gcgcctcacg accatcgcga cgctgattgg aatggaagcg cgcaagcctg
     1501 gcgcatacga ggtcacgaag ttcgagaccg tcgatgacaa aggcacgccg gtctgcacga
     1561 cttactcggg aagcatctat cgccaggttg agataagggg agcagagcgg ccggctgcta
     1621 tgccgaagcc gcccaagctt gcgaagctgc cggcgaaacc gcgcgcagag attaaagttg
     1681 cgatcccggc cggtgcagca cacgtctaca ccgagtgcgc gcgtatctgg aacccggtgc
     1741 atactgatgc cgcggttgcg gccgccgccg ggcttcccgc gatcattctg catggcacgg
     1801 cgacgctggc gatggcggtc tcacgtatcg tcatggctga agcgggcggg gacccggagc
     1861 gcatcgtgga gatttacggg cgattcggcg cgatggtcct tatgccgtcg gaggtgatgg
     1921 tgcgaatcca tgcacgcgag aaggccagcg gcggcgaggc cgtgctcttc ga
//
LOCUS       DASWGM010000640         6824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_81350,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000640 DASWGM010000000
VERSION     DASWGM010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81350"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VGA15_18010"
     CDS             complement(<1..164)
                     /locus_tag="VGA15_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HEX9589624.1"
                     /translation="MPSGPSSHSAMEIELAKLSSPRIFVVRMLVFLVLCALVMVVLYK
                     QIVTAFFANPG"
     gene            complement(506..1564)
                     /locus_tag="VGA15_18015"
     CDS             complement(506..1564)
                     /locus_tag="VGA15_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9589625.1"
                     /translation="MITPKVKTAFFAALNLAVLLTSLAVPAAMTHAQTPARSATIDPA
                     EEKRWLAVAPGRVEPLSGLIRVAPPVMGVIGEVLVRAGDTVFAGEPLIRLADHEARAR
                     LTAAEAQVALRRRVRNKESASSGAAARRRAEDAVADADIAVSEARSFVDKTAVERRAG
                     RGSDADVEAARSGLTRAQTRLVQQKAELRRIEADAPLPTQAEGQLNIARSELLATQAA
                     IENMTIRAPMDATILQMNARPGELASPSATQALVLLGNVSALRVRAEVDERDIEKIKA
                     GQAVLVRPAAFRDREIAGTVSFIAPLVEAGRYNALGQRNMTDVDVVEVLVNLGEPGPL
                     AVGMKVDVYFRQDGASRN"
     gene            complement(1561..2283)
                     /locus_tag="VGA15_18020"
     CDS             complement(1561..2283)
                     /locus_tag="VGA15_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589626.1"
                     /translation="MTEAVLDAVDVVKRLGRGAGEVEALKGVSLSLNGGELVLLMGPS
                     GSGKTTLLSVMGCLMTPTRGVIRVRGRSIAGAGPEDLAKLRRNHIGFIFQSYHLFPTL
                     TAEENVRLALDVRGQRFRRAKIAARESLAMVGLSHKTKAFPIELSGGEQQRVAIARAI
                     VGNACAILADEPTGALDTQNGQAIMTVLAEIAKEQSRGVLVVTHDPRILSFANRIVHI
                     EDGRIVREEPGERGKEQRVRMS"
     gene            complement(2280..3413)
                     /locus_tag="VGA15_18025"
     CDS             complement(2280..3413)
                     /locus_tag="VGA15_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589627.1"
                     /translation="MSLKFRLASRNLFHDRLRFVATIVGIVFSIVLVTVQMGLYVGFG
                     RMVTTMIDHAPADLWVMPLGTKCFEDPSLLDDRQRFRALAVDGVAEAAPLVIGFAQWR
                     LPGGGTTPVFIVGSELRGLGLRPWDLVAGRIDDLSIPGAVAIDQSYFDRLGTSGLGAS
                     AEIRDQKVQVTAVTKGIRSFTTTPFAFTGLDRARSYTGISPDKASYFLVRTSPGADVG
                     SVRQRLRANLSDVEVLTATEFRDRSRSFWLFGTGAGAALFAGALLGIIVGTVIVAQTL
                     YSSTKDHLDEFATLRAIGSSGAYIYTVIIWQALLSAVIGFCGAAAIGAIVVKATAESA
                     LPIVMTPALTVGLFLLTVAMCVASAIAAIMQVMRIDPALVFTR"
     gene            3468..4667
                     /locus_tag="VGA15_18030"
     CDS             3468..4667
                     /locus_tag="VGA15_18030"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX9589628.1"
                     /translation="MSTPGLALRNLRTVVIVIVLAVHAVMAYLGSSPASSFNFDDPPY
                     RWRSIPIVDLERWFGFDIFCAFQDVYLISLLFFLSGLFVWPSLVRSGSRIFLRDRLLR
                     IGVPFALTVGLLMPLAHYPVYRVTAVDPGLASFWQHWLALPFWPAGPPWFLWVLLVFD
                     FTAAVFYLFAPASGEAFGRSVGQLGACPRQFVTVLLSASAVAYVPLALIFNPWDWFHV
                     GPFSFQLCRPLHYLVYFFAGAGVGAYGIERGLLATDGWLVHRWAAAGAAAMVSFVLWL
                     GMVGLAIAGDDPPPLAVQILEALSFVICCSAGVLFMLALFLRFANRRLAALDPLKDKA
                     YGIYLIHYVFSIWLQYTLLGLAMFAVVKAMIVFTGTLLMSWASVAALRHIPARAWINR
                     SWRRLAT"
     gene            complement(4731..5525)
                     /locus_tag="VGA15_18035"
     CDS             complement(4731..5525)
                     /locus_tag="VGA15_18035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX9589629.1"
                     /translation="MLHSALINVMVKAARRAGRSLKRDLGEVENLQVSLKGPANFVTM
                     ADRRAEEMLHSDLTKARPGYGFIGEEGGIREGDDKSHTWIVDPLDGTTNFLHGIPQFA
                     ISIGLQREGTMIAGLIYNPANDDLYIAERGKGAFLNDQRLRVAGRRNLGDCVIACGLP
                     HIGRGDHNLSRLEMTEIQNKVAGLRRFGAASLDMAFVAAGRLDGYWERNLQPWDMAAG
                     QIIVREAGGTISGMAGDDDPLKTGNLICGNEFVHAELVKILKPLEK"
     gene            complement(5775..>6824)
                     /locus_tag="VGA15_18040"
     CDS             complement(5775..>6824)
                     /locus_tag="VGA15_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9589630.1"
                     /translation="ANAAAQISLVPPGVDTPPADKPAAKAKAKPAAAAKKPAVPVATP
                     NPPAPAATTNPAATPTAKVTPAPAPDDPRVDLVFGAYQRGQYKTAFDLASKRAQDTGD
                     PKAMTMLGELYANAMGIKRDYAKAADWYRRAADAGDREGMFALAMLRLSGRGGAVNRE
                     EAVKLLASSAKLGNPKAAYNLALLYLDGQTLPQDLTRAAELLRLAADAGNPEAQYALA
                     TFYKEGTGVKKDLDMAVLLLQAASLADNVDAEVEYAIALYNGTGTPKNEAAAIALLRK
                     AAKQNSPIAQNRLARVLVTGQGVPMDKVEGLKWHIVAKTAGRGDPMLDEALAALSPED
                     RAKAQQAARIWLGNR"
BASE COUNT         1173 a         2157 c         2108 g         1386 t
ORIGIN      
        1 ccgggattgg cgaagaaggc cgtgacgatc tgcttgtaga gcacgaccat gaccagcgcg
       61 cacagcacca gaaagaccag catgcggacc acgaaaatcc gcggcgagga cagttttgcc
      121 agttcgatct ccatcgcgga gtgggaagag gggcctgaag gcattgccga tgtcatccgt
      181 tgcgaggaag cggtttcggg catcaatatg gcacagcgcg gcggagaaaa aagctcggat
      241 acgccccgat ttcaagtgac atgcggaacc tctggctgaa acctaagcgt gggtgagagc
      301 gtacttcacg aatggttggt gtctagcgcg attttttttg attttgcatg accgttttgc
      361 atctcgaagc gctggccgcg atcgcggttt cgctttcgtc gctgtcgctg ccgaatcaaa
      421 tgatccaggg gattttcgta gcccttccgt tttggccggc aaacggcata ctattagcca
      481 tagcagcgtg caagcacgac gtaactcaat tgcgcgaggc gccatcctgg cggaaataca
      541 cgtccacctt catgccaacc gcgagcggac caggctcgcc caggttgacc agcacttcca
      601 ccacgtcgac gtcagtcata ttgcgttggc caagcgcatt atagcgtccg gcctccacca
      661 gcggcgcaat gaaggagacc gttcccgcga tttcgcggtc gcgaaacgcg gcgggacgca
      721 ccagcaccgc ttgtccggcc tttatttttt caatgtcacg ttcgtccacc tcggcacgca
      781 cgcgcaacgc cgaaacgttg ccgagcagca cgagcgcttg cgtcgccgat ggagacgcca
      841 gttcccctgg cctggcattc atttgcaaaa tggtggcgtc catcggcgca cgaatcgtca
      901 tgttttcgat agctgcctgg gtcgccaaca gttcactgcg cgcgatgttg agttggcctt
      961 cggcctgcgt tggcagcggt gcgtcggcct cgatcctgcg cagctcagcc ttttgctgga
     1021 ccagacgcgt ttgcgcacgc gtcaatcccg aacgggccgc ctccacatct gcatccgaac
     1081 ctcggccggc ccgcctttcg acggctgtct tgtcgacgaa agaccgcgcc tcggatacag
     1141 cgatgtcagc atcggccacc gcatcctcgg ccctgcgccg tgccgcagcc ccggacgagg
     1201 cggattcctt gttgcgcacg cgtcttcgca atgcgacctg cgcctctgcg gcggtaagtc
     1261 gcgcccgggc ttcatgatcg gccaggcgaa tcagcggttc accggcaaac acggtatcgc
     1321 cggcccgcac cagcacttcg ccgataacgc ccatgacagg cggggcaacc ctgatcaacc
     1381 ccgacaacgg ttcgacccgg cccggtgcga ccgccagcca tcgcttctcc tcggccggat
     1441 cgattgtcgc ggagcgtgcc ggcgtttgcg catgggtcat cgccgccgga acggcgagcg
     1501 aggtgagcag cacggccaaa tttagagcgg caaagaaagc cgtcttgacc ttcggcgtga
     1561 tcatgacatc cgcaccctct gttccttgcc gcgctcgccg ggctcttctc ttacgatccg
     1621 cccgtcttcg atgtggacga tgcgattggc gaacgacagg atacgcggat cgtgcgttac
     1681 gaccagcacc ccgcgagatt gctctttggc gatctcggca agcacggtca tgatggcctg
     1741 accgttttga gtgtcgagtg cgccggtcgg ttcgtccgca agaatcgcgc atgcattccc
     1801 cacaatggcc cgcgcgatcg cgacgcgctg ttgttcgccg ccgctgagct cgatgggaaa
     1861 cgccttcgtc ttgtgggaaa ggccgaccat cgccagggat tcccgtgccg cgatcttggc
     1921 gcgtctgaat cgctgtccgc gcacgtccag tgcgagccgg acattctcct cagccgtcag
     1981 ggtgggaaac agatggtagg attgaaagat gaagccgatg tgattgcgcc tgagtttggc
     2041 gagatcctcg ggacccgcac cggcgatcga ccggccgcga acccgtataa ctccgcgcgt
     2101 cggcgtcatc aggcatccca taaccgagag cagcgtggtc ttgccgctcc ccgatggtcc
     2161 catgagcagc accaactcgc cgccgttgag cgacaagctt acgcctttga gcgcctctac
     2221 ttcacccgct ccgcggccaa ggcgttttac gacgtcgaca gcgtcgagca cagcctcggt
     2281 catcgcgtaa acaccaaggc gggatcgatg cgcatgacct gcatgatcgc ggcaatggcc
     2341 gatgcaacac acatcgcgac ggtcagcaga aacaatccca cggtcaacgc cggtgtcatg
     2401 acgattggca atgcagattc agcggtcgcc ttgacgacga tggcgccgat ggccgcggcg
     2461 ccgcagaatc cgatgaccgc gctgagcagc gcctgccaga tgatgaccgt gtagatatat
     2521 gcgccggacg atccgatggc gcgcaaggtt gcgaattcgt ccagatgatc cttggtgctc
     2581 gagtacaagg tctgcgcgac tatgacggtg cccacgatga tgccgagcaa ggcgcctgcg
     2641 aacagagcgg cccccgcacc ggtcccgaac agccaaaagc tccggctgcg gtcgcgaaac
     2701 tcggtcgctg tcaggacctc gacgtcggag aggtttgccc gcaatcgttg acgaacgctg
     2761 ccgacgtccg ccccaggcga agtgcgcacg aggaaataag aggccttgtc gggtgaaatg
     2821 ccggtatagg atcgcgcgcg atcaagcccc gtgaacgcga agggcgtcgt ggtgaatgaa
     2881 cggatgccct tggtcaccgc ggtcacctga accttttgat cgcgtatttc agcgctggcg
     2941 cccaggccac tggtaccaag ccgatcgaaa taggactggt cgatcgccac cgcaccggga
     3001 atcgacagat cgtcaatacg acccgcgacc aaatcccagg ggcgcaatcc caatccccgt
     3061 agttcggagc cgacgatgaa aaccggcgtc gtcccgccgc ccggcaatcg ccattgcgcg
     3121 aagccaatga cgagaggagc ggcttcggca accccatcga ccgccagggc cctaaatcgc
     3181 tgacgatcat cgagcaacga aggatcttcg aaacacttgg taccgagcgg cataacccaa
     3241 aggtcggcgg gcgcatgatc gatcatcgtg gtgaccatgc gcccgaaacc gacatagagg
     3301 cccatttgca ccgtcaccag cacgatggag aagacaattc cgacgattgt tgcgacgaat
     3361 cgaagccggt catgaaacag gtttcgggag gcaagcctga acttcaacga catgacttgt
     3421 ggcccatccg aactttatct agtctatttc atctgcttta aagccatatg tccacgccgg
     3481 gactcgcgct gcgcaatctg cggactgttg tcatcgtcat cgtactggcg gtgcatgcag
     3541 tgatggccta tctgggttca tcgcccgcct cctcgttcaa cttcgacgat ccgccctatc
     3601 gatggcgaag tatcccgatt gtcgatctcg agcgctggtt cgggttcgac atcttttgcg
     3661 cgttccagga cgtctacctg atctcgctcc tgttctttct ctccgggctt ttcgtatggc
     3721 cgagcctggt gcgcagcgga agccgcatat tcctgcgcga tcgcctgctg cggatcggcg
     3781 tgccctttgc cttgacggtt ggcttgctga tgccgctcgc gcactacccg gtctaccgcg
     3841 tcaccgctgt cgacccgggc ctggcttcgt tctggcagca ttggctcgcc ttgccgttct
     3901 ggcccgccgg cccgccctgg ttcctgtggg tgctgctggt gttcgatttc acggcggccg
     3961 tattctacct attcgctccc gcatcgggtg aagcatttgg gcgatccgtt ggtcaattgg
     4021 gcgcctgtcc gaggcaattt gtcaccgtcc tgctgtcggc ctccgccgtc gcctatgttc
     4081 cgctggctct tattttcaat ccttgggatt ggttccacgt cgggccgttc tcgtttcagc
     4141 tttgccggcc gctgcactat ctcgtctatt tcttcgctgg cgccggtgtc ggcgcctatg
     4201 gaatcgagcg cgggttgctg gcaacggacg gttggctggt gcatcgctgg gcagccgcag
     4261 gtgccgccgc gatggtttcc ttcgtgctct ggctcggcat ggtcggcctt gcgatagcgg
     4321 gtgacgaccc accgccgctc gccgtgcaaa tcctggaagc gctcagtttc gttatctgtt
     4381 gctcagccgg agtcctgttc atgctggcgc tgttcctgcg tttcgccaat cggcgtttgg
     4441 ccgcactcga tcctttgaag gacaaagcct atggcattta cctgatccac tatgtctttt
     4501 cgatctggct gcaatacacg ctgctcgggc tcgcgatgtt tgccgtcgtg aaggccatga
     4561 tcgtgtttac cggcacgctc ttgatgagct gggcgtcggt cgcagcactt cgccacatcc
     4621 cggcgcgcgc ttggattaac cgctcgtggc ggcgattggc gacatagcca tcgagccgcg
     4681 cgcccgttta ccgcaaacga tgttcgcgcc gggaaacaag caggccaaac ctacttctcc
     4741 agcggcttca gaatcttcac cagttcggcg tggacgaatt cgttgccgca gatcaagttg
     4801 ccggtcttga gcgggtcgtc gtcgccggcc atgccgctga tggtgccgcc ggcttcgcgc
     4861 acgatgatct gccccgcggc catatcccac ggctgcagat tacgttccca atagccgtcg
     4921 agacggcccg ccgccacgaa agccatgtcg agcgaggccg cgccgaaccg gcgcaggccc
     4981 gcgaccttgt tctggatttc ggtcatctcc aggcgcgaca ggttgtggtc gccgcgtccg
     5041 atatgcggca gcccgcaggc gatcacgcag tcgccgaggt tgcgccggcc ggcgacgcgc
     5101 aagcgctgat cgttaaggaa ggcgcccttg ccgcgttcgg cgatgtagag gtcgtcattg
     5161 gccggattat agatcaggcc ggcgatcatg gtgccctcgc gctgcaggcc gatcgagatc
     5221 gcaaattgcg gaatgccgtg caggaaattc gtggtgccgt cgagcggatc gacgatccag
     5281 gtgtggctct tgtcgtcgcc ttcgcggatg ccgccttcct cgccgataaa gccgtagccc
     5341 ggccgcgcct tggtgaggtc ggagtgcagc atctcctcgg cgcggcgatc ggccatggtg
     5401 acgaaattgg cgggtccctt cagcgacacc tggagattct cgacctcgcc gaggtcgcgc
     5461 ttgagactgc ggccggcgcg gcgcgcggct ttgaccatga cgttgataag ggctgaatgc
     5521 agcatgatct cgacttgtaa ttgacgtttc gacggtgaaa gcgcgctccc tctcccatag
     5581 ggagagggca ggggtgaggg gttacagatt cagtgaggtt cgaaccccct cacccggcgc
     5641 tatccgacga cgcttcgcgt cgccgtgagc gccgacctct ccccgccggg gagaggtgag
     5701 acagcggccc gacgccagtc atcaaaccgg gccctagact gggtgccctg cggctgcggt
     5761 ggcgtcaagg cggatcatct gttgccgagc caaatgcgcg cggcctgttg ggccttggcg
     5821 cgatcctccg gacttaaggc cgccagcgcc tcgtcgagca tcgggtcacc cctgccggcg
     5881 gttttcgcga caatgtgcca tttgaggccc tcgaccttgt ccatcggcac gccctgtccg
     5941 gtcaccagca cacgtgcgag gcggttctgt gcgatcggac tgttttgttt ggcggccttg
     6001 cgcaacagcg cgatcgcggc ggcctcgttc ttcggcgtgc cggtgccgtt atagagcgcg
     6061 atggcgtatt cgacctcggc gtcgacattg tcggccagcg atgccgcctg caacagcagt
     6121 accgccatat cgagatcttt cttcacgccg gtgccctcct tgtagaaggt cgcgagcgca
     6181 tattgcgctt ccggattgcc ggcgtcggcg gcgaggcgaa gcaattccgc ggcgcgtgtc
     6241 aggtcctgcg gcagcgtctg tccgtcgagg tagagcagcg cgagattgta cgccgccttc
     6301 ggattgccga gcttggccga ggacgccagc aatttcacgg cttcctcgcg attgacggcg
     6361 ccgccgcgtc cgctgagccg aagcatggcg agcgcgaaca tgccttcgcg gtcgccggca
     6421 tcggcggcgc gcctgtacca gtcggcagcc ttggcatagt cgcgtttgat ccccatcgcg
     6481 ttggcgtaga gttcgccgag catcgtcatc gccttgggat cgccggtgtc ctgcgcgcgc
     6541 ttggaggcga gatcgaacgc ggtcttgtac tgcccgcgct gataggcgcc gaacaccagg
     6601 tcgacacggg gatcgtcagg cgcgggggcc ggagttacct tcgcggtcgg cgtggcggca
     6661 gggttcgttg ttgcggcggg agccggcgga tttggcgtcg cgacgggaac tgccggcttc
     6721 ttggccgccg ccgctggctt cgctttcgcc ttggcggctg gtttgtccgc cggcggcgtg
     6781 tcgaccccgg gaggcaccag cgagatttgc gccgccgcgt tcgc
//
LOCUS       DASWGM010000641         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1166488,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000641 DASWGM010000000
VERSION     DASWGM010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166488"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VGA15_18045"
     CDS             complement(<1..473)
                     /locus_tag="VGA15_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX9589631.1"
                     /translation="MNALFSPIKLRSVALPNRIMVAPMCQYSAENGEANDWHFTHINT
                     LALSGAAMFCIEATHVEAIGRITPGCLGLWNDATEAALKPILASVRKHSKTAVAMQLA
                     HAGRKGSSHTPWDGGQQIPLAEGGWQTEGPSAVPHKEGELAPLALDALGLKRVRDA"
     gene            complement(536..946)
                     /locus_tag="VGA15_18050"
     CDS             complement(536..946)
                     /locus_tag="VGA15_18050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9589632.1"
                     /translation="MGPRVPGCPLATALPLIREGQVRALLVSTSKRVPDLPDVPTPQQ
                     AGLKDAESAIWFGVFMPSKTPRDIIERFHAAGVKVLSEPAMQENLKKLGVEALPLTPV
                     EMDDLVKRETAATTEVIKAAGIKPWGDQAMVPCG"
     gene            complement(1094..>1567)
                     /locus_tag="VGA15_18055"
     CDS             complement(1094..>1567)
                     /locus_tag="VGA15_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX9589633.1"
                     /translation="LPRNKGIEALVMVPKGLPLAGTLIALSERGLDAKGNLIAFLIGG
                     KRPGQFAVRRTNNFDISDAVLLSSGALLILERKFSWLSGIGIRIRNIPLNAVAPGALV
                     DGPAIFDADLSHEVDNMEGIDVHVTPEGDTVLTMISDDNFSLLQRTLLLQFTLVE"
BASE COUNT          280 a          505 c          489 g          293 t
ORIGIN      
        1 gcgtcgcgca cgcgcttaag ccccaacgca tcgagcgcga gcggcgccag ttcgccctcc
       61 ttgtgcggca ccgcggaggg tccttcggtc tgccagccgc cttcggcgag cgggatttgc
      121 tgcccgccat cccatggcgt atggctcgat cccttgcggc cggcatgggc gagctgcatc
      181 gccaccgctg tcttggaatg cttgcgcacc gaggccagga tcggctttag cgccgcctcg
      241 gtggcatcat tccacaggcc gagacatccc ggcgtgatgc ggccgatcgc ttccacatgg
      301 gtcgcctcga tacagaacat cgcagcgccc gacagcgcga gcgtgttgat atgggtgaaa
      361 tgccagtcat tggcctcgcc gttttcggcg gagtactggc acatcggcgc caccatgatg
      421 cggttgggaa gcgcaacgct acgcagtttg atcggagaga acagggcatt catgggggga
      481 tgtccggtgg gagggaagtt cgttgctctc tatctagcga ggcgccgcgc tttcctcaac
      541 cgcatggcac cattgcttga tcaccccatg gcttgatccc ggcggccttg atcacttccg
      601 tggtggccgc cgtctcgcgc ttgaccagat cgtccatttc cacaggcgtc agcggcagcg
      661 cttcgacgcc gagctttttc aggttttcct gcatcgcggg ttccgacagc accttgaccc
      721 cggcagcatg aaatctttcg ataatgtcgc gcggcgtctt cgacggcatg aacacgccga
      781 accagatcgc gctctcggca tctttcaacc cggcctgctg cggcgtcggc acgtcaggca
      841 gatcggggac acgctttgat gtcgaaacca atagcgcgcg cacctgacct tcgcggatca
      901 ggggcagcgc ggtcgcgagc ggacagcctg gaacgcgggg acccataacc actggccttg
      961 ttgatagctg aaggcatttg ccccactgct caaacgagag gacacggagt gtgggtcccg
     1021 gcgtccgccg ggacgacaaa atttaggccc acgcaaaact cgcgtcatcg ccgcgtccgc
     1081 cgggacgacg agctcactcc accagcgtga actgcagcaa cagtgtgcgc tggagcagcg
     1141 agaaattatc gtcggagatc atcgtcagca cggtgtcgcc ctcgggcgtg acgtgaacgt
     1201 cgatgccttc catgttgtcg acctcgtggc tgagatcggc atcgaagatc gcggggccat
     1261 cgaccagcgc ccccggcgcc accgcgttga gcgggatgtt gcggatccgg atgccgattc
     1321 cagagagcca tgaaaacttc cgttccagta tcaggagtgc acccgaggac agcagcaccg
     1381 cgtcgctgat atcgaaattg ttggtgcggc ggaccgcgaa ctggccgggc ctcttgccgc
     1441 cgatcaggaa tgcgatcagg ttgcccttcg catcgaggcc gcgctccgaa agcgcgatca
     1501 gcgtgccggc cagcggcagg cccttcggca ccatcaccag cgcctcgatc cccttgttgc
     1561 gaggcag
//
LOCUS       DASWGM010000642         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1437596,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000642 DASWGM010000000
VERSION     DASWGM010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437596"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..303)
                     /locus_tag="VGA15_18060"
     CDS             complement(19..303)
                     /locus_tag="VGA15_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9589634.1"
                     /translation="MLSAKSDVQVDTPEVRVTEWRLAPGSATGHHIHEMDYVIVPVTS
                     GEMTIVAPGGERSKAQLGLGKSYFRKAGVEHDVLNETASEIVFLEVELKP"
     gene            complement(322..1419)
                     /locus_tag="VGA15_18065"
     CDS             complement(322..1419)
                     /locus_tag="VGA15_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEX9589635.1"
                     /translation="MSAHGPMPRSAWIFPVLAVLFFAGATALGLTFTPSAAGLGFAVV
                     LLIILFGTVFAAVHHAEVIAERIGEPYGTLLLTLAVTIIEVALIATIMLGEKPVPTLA
                     RDTVFAVVMIVCNGLVGICILAGGLRYREQDVQVTGSSLYLSVLIVIATITLIMPNYT
                     LTTPGPIYSAAQLGFVSVVTIILYGVFLYTQTIRHRNYFVSEAAGVADGGTLISNRLL
                     GLSMALLLVSLLAVVLLAKKFSLVVDFATARIGAPPVFAGVLVALLILLPESVAAIAA
                     ARKNDLQKSINLALGSSLATIGLTIPAVAVAAYALDKQLVLGLDTQAMVLLAMTFILS
                     MLTFGTGRTNILFGLVHLVVFAVFIFLVFAP"
     gene            complement(1474..2280)
                     /locus_tag="VGA15_18070"
     CDS             complement(1474..2280)
                     /locus_tag="VGA15_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9589636.1"
                     /translation="MTVLKWFVILAIVGYLGGLAVLFFKQREFIFPIPQTVRTAPEAA
                     GFPEAEEHVLATADGEKVIIWHVPAQPGHAVVIYFPGNGDFLAGSVGRFRAIASDGTG
                     LVALSYRGYAGSSGQPSERGLLQDAAAAYAFTAARYTPDRIVLWGFSLGTGAAVAVAA
                     EQPVGKLILEAPYTSMADVAAWHFPVLPVRWLLRDRFHSDRRMARVTVPLLVMHGERD
                     LTIPIEFGERLFGLAPGPKQFVRFPLGGHDDLGNHGAISTARQFIGGPNG"
     gene            complement(2299..3699)
                     /locus_tag="VGA15_18075"
     CDS             complement(2299..3699)
                     /locus_tag="VGA15_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX9589637.1"
                     /translation="MNADLAAVLYLVAGVLFILSLRGLSSPASSRRGNFLGMTGMGIA
                     IATTLAAHPPADGIGWLLVVLGVAIGGSIGAVIARRVPMTSMPELVAAFHSLVGMAAV
                     LVAAGAFYAPEAFDIGTPGNIHGASLVEMSLGVAIGALTFTGSVIAFAKLSGRMSGAP
                     IILPARHVINIALAIALVVFIVGLVLTGSALDFWLITIIALALGALLIIPIGGADMPV
                     VISMLNSYSGWAAAGIGFTLGNSALIITGALVGSSGAILSYIMCHAMNRSFISVILGG
                     FGGETAAAGAGAGGEVRPVKLGSADDAAFIMKNAQKVIIVPGYGMAVAQAQHALREMA
                     DQLKKEGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINSEFAQADVAFVI
                     GANDVTNPAAEDDSKSPIYGMPVLQVWKAGTVMFIKRSLASGYAGIDNPLFYRDNTMM
                     LLGDAKKMTENIVKAL"
BASE COUNT          830 a         1195 c         1183 g          620 t
ORIGIN      
        1 aggcgccaaa tggcggggtt atggtttcaa ttccacttcc agaaacacga tttcggacgc
       61 ggtctcgttg agcacgtcat gttccacccc ggccttgcgg aagtaggatt taccgaggcc
      121 aagctgcgcc ttggagcgtt ctccgccggg cgccacgatg gtcatttcgc ccgaggtcac
      181 gggaacgatg acataatcca tctcgtgaat gtgatgaccg gtggcgctgc ccggcgccag
      241 ccgccactcg gtgacgcgga cttccggcgt atcgacctga acatctgatt tggcgctaag
      301 cattatcgtt cactccaact ctcacggcgc aaaaaccagg aaaatgaata ccgcaaacac
      361 caccaagtga accagcccga acaggatatt ggtgcggccg gtgccgaagg tgagcatgct
      421 gaggatgaag gtcatcgcca gcagcaccat ggcctgagta tcgagcccaa gcaccagttg
      481 cttgtccagc gcgtaggccg ccaccgcgac cgccgggatc gtcagcccga tggttgccag
      541 cgaagaaccc agcgccagat tgatgctctt ctgcagatcg ttcttgcgcg cggcggcgat
      601 ggcggcgacg ctttccggca gcaatatcaa cagcgccacc aggacgcccg cgaagacagg
      661 cggcgcgccg atccgcgcgg tcgcaaaatc gaccaccagt gagaatttct tcgccagcag
      721 caccaccgcc agcagcgaga ccaggagcag cgccatgctc aagcccaaca gccggttcga
      781 aatgagcgtc ccgccatccg cgacgccggc ggcttcgctg acgaaataat ttcgatgccg
      841 gatggtctgg gtatagagaa acaccccata aagaatgatg gtgacgacac tgacaaaacc
      901 gagttgggcc gccgagtaaa tcggccctgg cgtcgtcagc gtgtaattgg gcatgatcaa
      961 ggtaatggtc gcaatgacga tcaggacgct gagatagagg cttgatcctg tgacctgaac
     1021 gtcctgctcg cggtagcgca gacctccggc caggatacag atcccgacca ggccgttgca
     1081 cacgatcatc accacggcga acaccgtgtc gcgcgccaag gtggggacgg gcttttcacc
     1141 gagcatgatg gtcgcgatca gcgcgacttc gatgatggtg accgccagcg tcaacaacag
     1201 cgtgccgtag ggctcgccga tccgctcggc gatcacctcg gcatggtgca cggccgcgaa
     1261 cacggtgccg aacagaatga tcagcaagac caccgcgaac ccaagcccgg ctgccgaggg
     1321 cgtgaaggtg agaccgagcg cggtggcgcc agcgaaaaac aggaccgcca gcacgggaaa
     1381 gatccacgcc gatcgcggca tcgggccgtg cgcgctcatg ctgtcacatc ctctgggcgt
     1441 ggatatcgat tatgcaccaa atgactgccg cggtcagcca ttcgggccgc caatgaactg
     1501 tcgtgccgtc gagatcgcac catgattgcc gagatcgtcg tggcctccta acggaaaccg
     1561 cacgaattgt ttcggccccg gggcgagccc gaacagacgc tcgccgaact caatcggaat
     1621 ggttaaatcg cgttcgccat gcatgaccag gagcggaacg gtgacgcgcg ccatgcgccg
     1681 atcgctgtga aaccgatccc gcaacagcca gcgcaccggc agaaccggaa aatgccaggc
     1741 ggcaacatcc gccatcgatg tatagggagc ttccaggatc agctttccaa ccggctgttc
     1801 ggctgcaaca gcaaccgcgg cgccggtgcc gagcgaaaag ccccacagca cgattcgatc
     1861 gggcgtgtat cgcgcggccg tgaaagcgta agccgcggcg gcgtcttgga gcagtccccg
     1921 ctcgctgggc tggccgctgg agccggcata gcctcgatag gacaacgcga cgagcccggt
     1981 cccatcagag gcgatggccc gaaagcggcc gacgctgccg gcgaggaaat ctccattgcc
     2041 gggaaaatag atcacaaccg catggcccgg ttgcgccgga acatgccaaa tgatgacctt
     2101 ctcgccatcg gccgtggcca gaacatgttc ttcagcttcc ggaaagccag cggcttcagg
     2161 ggccgtacgt accgtttgcg gtatcggaaa gatgaattcc cgttgcttga agaaaaggac
     2221 ggcaagaccg ccgagatagc cgacgattgc gagaatgacg aaccatttca ggacggtcat
     2281 gatttctcgt gaacagcctc acagcgcctt gacaatattc tcggtcatct tctttgcatc
     2341 gccgagcagc atcatggtgt tgtcgcggta gaacagggga ttgtcgatgc cggcatagcc
     2401 cgacgccagc gagcgcttga tgaacatcac ggtgccggcc ttccagacct gcagcaccgg
     2461 catgccgtag atcggcgatt tcgaatcgtc ttcggcggcg ggattggtaa cgtcattggc
     2521 gccgatcacg aaggcgacgt cggcttgcgc gaattccgaa ttgatgtcct cgagttcgaa
     2581 cacctcgtcg tagggcacat tggcttcggc gagcagcacg ttcatgtggc ccggcatgcg
     2641 gccggccacc gggtggatcg cgtacttgac ctcgacgcct tccttcttga gctggtcggc
     2701 catttcgcgc agcgcatgct gcgcctgcgc caccgccatg ccgtagccgg gcacgatgat
     2761 caccttctgc gcattcttca tgatgaaggc ggcgtcgtcg gcggagccga gtttcacggg
     2821 ccggacttcg ccacccgcgc cggcgcccgc ggcggccgtc tcgccgccaa aaccgccgag
     2881 aatgaccgag atgaaggaac ggttcatcgc gtggcacatg atgtaggaca ggatcgcgcc
     2941 ggaggagccg accagcgcgc cggtgatgat cagcgccgag ttgccgagcg tgaagccgat
     3001 gccggcggcg gcccagccgg aataggagtt cagcatcgag atcacgaccg gcatgtcggc
     3061 gccgccgatc gggatgatca ggagcgcgcc gagcgccagc gcaatgatgg tgatcagcca
     3121 gaagtcgagc gcgctgcccg tcagcacgag gccgacgatg aacaccacca gcgcaatcgc
     3181 caatgcgata ttgatgacgt gacgtgcggg cagaataatc ggcgcaccgc tcatgcgccc
     3241 cgacagtttt gcaaacgcga tcaccgagcc ggtaaacgtc agcgcgccga tggcgacgcc
     3301 gagcgacatt tcaaccaggc tagcgccatg gatgttgccg ggcgtgccga tgtcgaaggc
     3361 ttccggcgcg tagaacgcac cggccgcgac cagcaccgcg gccatgccga ccagcgagtg
     3421 aaacgccgcg accagctccg gcatcgacgt catcggcacc cggcgcgcaa tcaccgcgcc
     3481 gatcgagccg ccgatggcga cgccgagcac caccagcagc cagccaatgc cgtcggctgg
     3541 cgggtgcgcg gccagcgtgg tcgcgattgc gattcccata ccggtcatgc caaggaagtt
     3601 accgcggcgg gatgaggccg ggctcgacag cccgcgcagc gacagaatga acaggacgcc
     3661 ggcgaccaga tacaggactg cagcgagatc ggcgttcatg atgcgcgcac tccgtcatgc
     3721 gcgggctcga cccgcgcatc catctgaata aaaagaatct tgcgaagcgc gatggattgc
     3781 cgggtcaagc ccggcaatga cgacgatatc ttctgaaaaa gattcata
//
LOCUS       DASWGM010000643        10323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2018565,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000643 DASWGM010000000
VERSION     DASWGM010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018565"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1222
                     /locus_tag="VGA15_18080"
     CDS             245..1222
                     /locus_tag="VGA15_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9589638.1"
                     /translation="MRRLLTVTVVALVGLAGAGMAAAQNYPMRAMTVIVPFPAGGATD
                     TLARFLAEKMRAVLGQPVIIENVAGAAGSLGVGRAVRSAADGYTLSIGTSTTHMLTGG
                     LYALQFDLLKDLEPVIQIGSEPLLIVGRKSLPADDLKGLIAYLKANPDKASVGIAGVG
                     ATGHLTGISFQKETGTKFQFVPYRGNAPAMQDLLAEQIDFMIEPSSNFKSLVGAGSVK
                     PYAITGRTRLPSSPDIPTADEAGLAGFFASLWYGLWVPKDTPKDIIAKLNATMVQVLA
                     DPAVQKRFDELGIQITPLTQQSPEALRGFQKAEAERWWPIIKASNIKSE"
     gene            1297..2181
                     /locus_tag="VGA15_18085"
     CDS             1297..2181
                     /locus_tag="VGA15_18085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX9589639.1"
                     /translation="MKIHAIQTGSVRIKTAQVEGQGHGLSRRLAIFADRDWTDWLPTY
                     AWVIDHPEGIIVVDTGQGAHLLESDKSLHPYIRWEVAFRIEREQEIGPQLRALGIGPR
                     DVKRVVLTHLHMDHDGGLAHFPNTEILVAPGELQTARGMLGRIRGYLPQRWPTWFDPK
                     PLHLAAEPSGPFAMSRRLTEAGDVIAVATPGHTADHISVIIQDSEITYFLAGDTSYNE
                     ELMLAGKLDGASADEEVATATLDAIRRFAQSRPTVYLPTHDPGAADRLANRRSAGTGY
                     AASARFSPASAGSERSPA"
     gene            complement(2135..3094)
                     /locus_tag="VGA15_18090"
     CDS             complement(2135..3094)
                     /locus_tag="VGA15_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX9589640.1"
                     /translation="MPTILGSGEHRYRVVENWAKLPEGWNLSDVASVAVDSKDRIYVF
                     NRGAHPMVVLDREGNFITSWGEGLFRRAHGLHIDADDNLYCTDDGDHTVRKCSVDGKV
                     LLTIGIPNKPAPFMSGEPFHRCTHTALSPKGEIYVSDGYGNACVHKYTPDGKLIKTWG
                     ESGTDPGQFNIVHNIVTDQDGWVYVADRENHRVQVFDGNGKYEAQWNNLHRPCALHCC
                     GGRQPNFIIGELGPGMPVNLKAPNLGPRLTIVDSKGKRIARLGGEDGPGLETGKFLAP
                     HGIALDSKGDIYLGEVGVTNWKTSFPDTPMPAIVRCLQKLEKI"
     gene            complement(3227..5017)
                     /locus_tag="VGA15_18095"
     CDS             complement(3227..5017)
                     /locus_tag="VGA15_18095"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9589641.1"
                     /translation="MLDRTDDVSIATENWLTQFESALATSDDTLLKTLFHPESHWRDV
                     LALSWNIQTINGAEAILRELKADAGRARPAGFAIDPDRAAPRRVKRAGTSTIEAIFKF
                     EIAQGRGNGILRLVPDAGDGNRPKAWTLLTALDELKGFGEQVGTSRPRGQAYSRDFRG
                     PNWLDLRKAAAEYADHDPAVLVIGGGQSGLCIAARLKQLQVDTLIVDRGLRIGDNWRN
                     RYHALTLHNQVQVNHLPYMLFPPNWPTYIPKDKLANWFEAYVESMELNYWTATEFESG
                     SYDEKEQRWSVVLRRADGSKRTMHPRHVVMATGVSGIPSLPDIPTLKNFTGAVIHSSQ
                     YTDGEEWRSKKALVIGTGNSGHDIAQDLHSSGAQVTLVQRSSTLIVSIEPSAQLVYAP
                     YNEGTLDDNDLIATSMPLAVARKSHAMVTEHSKRLDQELLDGLERVGFKLDFGDGDTG
                     WQFKYLTRGGGYYFNVGCSDLVVSGEIALKQFSDIEGFVAEGARMKRGETLAADLIVL
                     ATGYKRQEELVRKLFGDDVVDRIGPIWGFGESQELRNMYTRTAQPGLWFIAGGLAQCR
                     INSKYLALQIKAVEEGLLPRVVRSEDAVCA"
     gene            complement(5115..6206)
                     /locus_tag="VGA15_18100"
     CDS             complement(5115..6206)
                     /locus_tag="VGA15_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HEX9589642.1"
                     /translation="MKSTDFIVARNDLQECKVIETSLPEAADLPDEALLVKVDRFAFT
                     ANNITYAVLGDQLKYWQLFPAPENFGNIPVWGFGEVIASRHPGIVIGERLFGYFPMAT
                     HLVIEAADVSKRGLRDAAAHRQGVAPVYNAYARVGGDPAFAGRRGDHQALLRPLFMLS
                     FLVDDFLSENEFYGARSVMLSSASSKTAFGLAHLLHTQHKDIRVIGLTSAANADFVRS
                     LGCYDDVVTYDRVTSLPLDSPVAFVDMAGNSALRANLHRHFGNQMKYSGRIGLTHRAS
                     SPDEAELPGARPSWFFAPDQIRKRAREWGPGGIDTRFGKAWAGFTPMLDRWLAVAEGR
                     GEGAVKQVYLDTLNGRVPPDQGHILSLGT"
     gene            complement(6203..6949)
                     /locus_tag="VGA15_18105"
     CDS             complement(6203..6949)
                     /locus_tag="VGA15_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX9589643.1"
                     /translation="MTLHLNTQADLEQAIHALIKQDPRLKPIFEVAGMPALRRRQPGF
                     AGLAAIVSGQQLSTASAAAIWARVSAAFDPFDHDSLRRARADRLGRLGLSAAKIKTLK
                     GVARELAAGRLNFDVLANEDADATHHTLTALHGIGPWTADVYLLFCLGHGDAWPAGDL
                     AVQEAVKIGLGLKTRPTVKEMAPLAEPWRPMRGAAAHLWWTYYRVLKKREGVIGGQQK
                     ANAVLNKISVAKRSSTNAKIAAAKLPGSNK"
     gene            7143..8015
                     /gene="gluQRS"
                     /locus_tag="VGA15_18110"
     CDS             7143..8015
                     /gene="gluQRS"
                     /locus_tag="VGA15_18110"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX9589644.1"
                     /translation="MPPPVFRFAPSPNGYLHLGHALSALLNFDSARRSGGRFLLRIED
                     IDATRCRPEFETAIYEDLAWLGIAWETPVRRQSEHLSEYRDAVERLAAEGLVYPSFES
                     RGEIARLVAQREAEAPWPRDPDGTPLYPGAAKSLPADQRARLLESGALYALRLDMAAA
                     RARAGDLTWLEHGEGPDGETGRVAARPQAWGDVILARKEAPTSYHLSVVIDDARQGVT
                     EVVRGEDLFWSTSVHRLLQQLLGLPHPVYRHHRLVRDGEGRKLSKSTMATGLRELRAA
                     GATPADIRRGVGLP"
     gene            8264..9409
                     /locus_tag="VGA15_18115"
     CDS             8264..9409
                     /locus_tag="VGA15_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9589645.1"
                     /translation="MIPVPSMVEAALAAFAHEVRTPLTGILAISNLLATSDLDERERR
                     WVDTIKAGAEHLASLATLFVDAARSGNAAAAVRQDFFDLRALARSIGDSLAGRAAAKG
                     LQSSIDISEKLPAPVVGDPVRLRAALENLIDNAVKFTETGGVALTVKAVRGPKGKVGV
                     SFAISDSGIGLTLNEIKRLFRPFSQANVSIASRFGGAGLGLSSVKQLARVMGGDIVVS
                     QRRNGGATFTLTVTLSRARASKAAGADRENDALSDPERPLRVLSVEDNPFGRVVLNAV
                     LTELGHSAEFIGRGEAAAERIAQGAFDAVLMDMVLPGINGIEAIKQIRELDAPLGGIA
                     IIGVSGRGDDEAASRAAGADAFLVKPVSPRALATALLEATRRAATAT"
     gene            complement(9336..10229)
                     /locus_tag="VGA15_18120"
     CDS             complement(9336..10229)
                     /locus_tag="VGA15_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX9589646.1"
                     /translation="MRQLRYIFHVGMDAFYTFLADDGWAIASHIALSTLMALFPFLIV
                     LTSLAGFFGSKELADQAAGLMLEVWPKQVADSLSGEIHDVLTTTRGDALTIGAVLAVY
                     FASNGVESLRVALNRAYSVIEPRRWYWLRLESIGYTLIAAFTALAMAFLIVLGPLMLE
                     AARRYIPLIVESNENFLNLSRYGIAIAALVVALFVLHAWLPAGRRGFLQILPGIIFTL
                     VASLLSGIVFGQYLARFANNYVTMYAGLASVIIALVFLYFIAAIFVYGGELNAAIIKS
                     RLPHGVSLQAAQSLKPLDSQA"
BASE COUNT         1910 a         3238 c         3258 g         1917 t
ORIGIN      
        1 catgaacatc caggaagcca tcgctgtttt tgacgccgcg gaagccgagt cgcttgtcgt
       61 ggtcgaaacc ggcggcgaac cgcggccgat cggaattctc agcgaagccc acgcgatgcg
      121 gcgctatgcg gaggaatccg aacaacggcg gcgcgaagcc gtcggcgata tctagtcagc
      181 gctgcggaaa agcgcgacaa gcagggtcaa cgtgcattgc ataagcacat cccaagggaa
      241 ccccatgcgc agattgctga cggtgacggt ggtggccctt gttggcctgg caggggccgg
      301 catggccgcc gcccaaaact atccgatgcg tgcgatgacc gtgatcgtgc cgttccctgc
      361 ggggggagca accgacacct tggcgcgatt cctggcggag aaaatgcgag ctgttctcgg
      421 tcaacccgtc atcatcgaga acgttgcggg agcagccggc agtctcggcg tcggccgcgc
      481 cgttcggtca gccgccgatg gctacaccct gagcatcggc acttcgacca cgcatatgct
      541 gacgggcggt ctctacgccc ttcagttcga tctgttgaaa gatctggaac cggtcattca
      601 gatcggcagc gagccgcttt tgatcgtcgg cagaaagagc ctgccggcag atgatctgaa
      661 agggctgatt gcctatctca aggccaatcc ggacaaggca tcggtcggca ttgccggggt
      721 cggcgcgaca ggccatctga ccggcatttc gttccagaag gaaaccggaa ccaagtttca
      781 attcgtgcca tatcgcggca atgcgccggc catgcaggat ctgttggcgg aacagatcga
      841 tttcatgatc gagccgtcgt cgaatttcaa gtcgctggtc ggcgccggaa gcgtcaagcc
      901 ttacgccatc acgggccgga cgcggttgcc ttcgtcgccg gatattccaa ccgcggacga
      961 ggcgggtctt gccggcttct ttgcctcgct ctggtacgga ctttgggtgc ccaaggacac
     1021 gccaaaggac atcatcgcca agctcaatgc caccatggtg caggtgctgg ccgatcccgc
     1081 ggtgcaaaaa cgctttgacg aacttggcat ccagatcacg ccgctcaccc agcaatcgcc
     1141 ggaagccctg cgcgggttcc agaaggccga ggcggaacgg tggtggccga tcatcaaggc
     1201 gtcaaacatc aagtcggaat gagtggcgag gtttttgcag gcccgcgaca aagacgacac
     1261 cgcgaaatcg gcgcatttgg agttttgttt gcagccatga agattcacgc cattcagacc
     1321 ggctccgtcc ggatcaagac cgcgcaggtc gaggggcagg ggcatggcct gtcgcggcgg
     1381 ctcgcaattt ttgccgatcg ggactggacc gactggctgc caacctatgc ctgggtcatc
     1441 gatcatcccg aaggcatcat cgtggtcgac accgggcagg gcgcgcatct gctggaatcc
     1501 gacaagtcgc ttcaccctta tatccgctgg gaagtggcgt ttcgcatcga gcgcgaacag
     1561 gagatcggtc cgcagctgcg cgcgctcggc atcggaccgc gcgatgtgaa gcgggtggtc
     1621 ctcacccatt tgcacatgga ccatgacggc ggcctcgcgc attttccgaa caccgaaatc
     1681 ctcgtcgcac caggcgaatt gcaaaccgcg cggggaatgc tcggccggat acgcggctat
     1741 cttccgcagc gatggccgac atggttcgac ccaaaaccgc tgcatctggc ggccgaaccg
     1801 tccggcccct ttgccatgag ccgtcgcttg acggaagccg gcgacgtcat cgcggtggcg
     1861 acgcccggcc acaccgcgga tcatatctcc gtcattatcc aggacagcga gatcacgtat
     1921 ttcctggccg gcgatacctc atacaatgag gaactgatgc ttgccggcaa gctcgatggc
     1981 gccagcgcgg acgaagaggt cgccactgcg acgctggatg cgatcaggcg tttcgcccaa
     2041 tcgcgtccga ccgtatatct gccgacgcac gatcctgggg ccgccgaccg tctggccaac
     2101 cggagatcag cggggactgg gtatgcggcg tccgctagat tttctccagc ttctgcaggc
     2161 agcgaacgat cgccggcata ggcgtatccg gaaagctggt cttccagttg gtgacgccga
     2221 cttcgccgag atagatgtcg cccttcgaat ccagcgcgat gccgtgcggc gcaagaaact
     2281 tgccggtctc gaggccagga ccgtcctcgc cgccgagccg tgcgatgcgc tttcccttgg
     2341 agtcgacgat ggtcaggcgg gggcccagat tgggcgcctt caggttgacc ggcatgcccg
     2401 gaccaagctc gccgatgatg aaattcggct gcctgccgcc gcagcaatgc agcgcgcagg
     2461 gccggtgcag attattccac tgcgcctcgt atttgccgtt gccgtcgaac acctgcacgc
     2521 ggtggttctc gcggtccgcc acgtaaaccc agccgtcctg gtcggtgacg atgttgtgga
     2581 cgatgttgaa ctggcccgga tcggtgccgg actcgcccca ggtctttatc agtttgccgt
     2641 caggcgtgta tttgtggacg caggcgttgc cgtagccgtc cgacacgtag atttcgccct
     2701 tcggcgacag cgccgtgtgg gtgcagcggt ggaacggctc gccgctcatg aacggtgccg
     2761 gcttgttggg gatgccgatg gtcagtagca ccttgccgtc aaccgagcat ttgcgcaccg
     2821 tatgatcgcc gtcatcggtg caatagagat tgtcgtcggc atcgatgtgc agcccgtgcg
     2881 cgcggcggaa cagcccttcg ccccagctcg tgatgaaatt gccctcgcgg tcgagcacga
     2941 ccatcgggtg cgcgccgcga ttgaagacgt agatacgatc cttgctgtcg actgcgacgg
     3001 acgcgacatc gctgaggttc cacccctccg gcagcttcgc ccagttctcc accacgcggt
     3061 agcgatgctc gcccgagccg agaatggtcg gcatgtgttt tctcccctgc ttttgtcgtc
     3121 attccgggat gcgcctcttg gcgcaggccc ggaatccata ctccccgtcg gggttatgga
     3181 ttccgggctc gtccttcgca cgccccggaa tgacgggact ttcatgtcac gcgcataccg
     3241 cgtcctctga gcgcacaacc cgcggcagca gcccctcctc gacggccttg atctgcaacg
     3301 ccaaatactt tgaattgatg cggcattgcg ccagaccgcc ggcgatgaac cacaggccgg
     3361 gctgcgcagt gcgggtgtac atgttgcgca actcctggct ctcgccgaat ccccagatcg
     3421 ggccgatgcg atcgacgaca tcgtcgccga acagttttcg caccagttct tcctggcgct
     3481 tgtagccggt cgccagcacg atcagatcgg cggcaagcgt ctcgccgcgc ttcatccgcg
     3541 cgccctcggc gacaaaacct tcgatatcgg aaaactgctt cagggcgatc tcgccgctga
     3601 cgacgagatc ggagcagccg acgttgaaat aatatccgcc gccgcgggtg agatatttga
     3661 actgccagcc ggtgtcgccg tcgccgaaat cgagcttgaa gccgacgcgc tccaatccat
     3721 cgagcaattc ctggtccagt cgcttcgagt gctccgtcac catggcgtgg cttttcctgg
     3781 ccaccgcgag cggcatcgag gtcgcgatca ggtcgttatc gtcaagcgtc ccctcattat
     3841 acggcgcata gaccaattgc gctgacggtt cgatgctgac gatcagcgtg gagctgcgct
     3901 gaaccagcgt gacctgcgcg ccgctggaat gcaggtcctg cgcgatgtcg tggccgctgt
     3961 tgccggtgcc gatcaccagc gcttttttgc tcctccactc ctcaccgtcg gtgtattggc
     4021 tcgaatggat caccgcgccg gtgaaattct tcagcgtcgg gatatcgggc agactcggaa
     4081 tgccgctgac gccggtggcc atcacgacat ggcgcggatg catcgtgcgc ttgctgccgt
     4141 cggcccggcg caacaccacc gaccagcgtt gttctttctc gtcgtagctg ccgctctcga
     4201 actcggtagc tgtccagtaa ttcaactcca tgctttcgac ataggcttcg aaccagttgg
     4261 cgagcttgtc cttcggaata taggtcggcc agttcggcgg gaacagcatg tagggcaggt
     4321 ggttgacctg cacctgatta tgcagcgtca gcgcatggta gcgattgcgc cagttgtcgc
     4381 cgatgcgcag cccccggtcg acgatcaggg tatcgacctg caattgcttc aggcgtgcgg
     4441 cgatgcaaag cccggactgt ccgccgccga tgacgagaac ggcgggatcg tggtcggcat
     4501 attcggccgc ggctttgcgc aggtcgagcc agttggggcc gcgaaaatcg cgcgaatagg
     4561 cctggccgcg gggccgagac gttccgactt gttccccaaa accttttagc tcgtcgagcg
     4621 ccgtcaacaa cgtccaggct ttcggcctgt tgccgtctcc ggcgtcgggg accagccgca
     4681 gaatcccgtt gccgcggccc tgcgcgattt cgaatttgaa gatcgcttcg attgtgctgg
     4741 tgccggcgcg ttttaccctg cgcggcgccg cgcgatcggg atcgatcgca aaaccggccg
     4801 gtcgggcgcg gccggcgtcg gctttcaact ctctcaagat ggcttccgcg ccgttgatgg
     4861 tctggatgtt ccagctcagc gccagcacat cgcgccaatg gctttccgga tggaacaggg
     4921 ttttgagcag ggtatcgtct gaggtcgcaa gcgcgctttc gaactgcgtc agccagtttt
     4981 ccgtggcgat cgacacgtca tccgttctgt cgagcattcg cgtttcccgt catttgccgt
     5041 cttcgcggcg cttgcctgaa cgcaaaacct acatcgtttg ggatagcgca gaaagggcgt
     5101 gacgagacgc cctgctatgt ccccagcgac aggatgtggc cctgatccgg cggcacccgg
     5161 ccattcaagg tatcgagata gacctgcttc accgcccctt cgccgcgacc ttcggcgacg
     5221 gcaagccacc gatccagcat cggcgtgaag cctgcccagg ctttgccgaa ccgcgtgtcg
     5281 atgccgccgg gtccccactc cctggcacgt ttgcggatct ggtcgggcgc gaagaaccat
     5341 gacggcctgg cgcccggcaa ttccgcctcg tcaggcgagg aggcgcggtg ggtgagcccg
     5401 atgcgtcccg aatatttcat ctggttgccg aaatgccgat gcaggttcgc gcgcaacgcg
     5461 ctgttgcccg ccatgtcgac gaaagcgacg ggtgaatcca acggcagcga ggtcacgcgg
     5521 tcataggtca ctacgtcgtc gtagcagccc agcgacctga caaaatcggc attggccgcc
     5581 gaggtcagcc cgatcaccct gatatccttg tgctgcgtat gcagcagatg cgcgaggccg
     5641 aacgcggtct tgctggaggc gctggaaagc atcacgctgc gcgcgccgta aaattcgttc
     5701 tcgctcaaga aatcgtcgac caggaacgac aacatgaaca gcggccgcag cagcgcctga
     5761 tgatcgcccc gccgtccggc aaaggccgga tcgccgccga cgcgcgcata ggcattgtag
     5821 accggcgcca ccccttgccg atgcgcggcg gcatcgcgca ggccgcgctt gctgacgtcg
     5881 gcggcctcga tcaccagatg ggtggccatc gggaaatagc cgaacaggcg ttcgccgata
     5941 acgatgcccg gatggcgcga ggcgatcacc tcgccaaatc cccacaccgg aatgttgccg
     6001 aaattttccg gcgccggaaa cagctgccag tattttagct gatcgcccaa caccgcgtag
     6061 gtgatgttgt tggcggtgaa ggcgaagcga tcgactttga cgagcagcgc ctcatcgggc
     6121 aagtccgcag cctcaggcag cgatgtctcg atgactttac attcctgcag atcgttgcgc
     6181 gcaacgatga agtcggtgga tttcatttgt tcgatcccgg cagttttgct gccgcgatct
     6241 ttgcgtttgt cgatgaccgt tttgcaacag atatcttgtt taaaacggcg ttcgcttttt
     6301 gctgtccccc gatcacgcct tcgcgctttt tcagcaccct gtagtaggtc caccacagat
     6361 gcgccgcggc gccgcgcatc gggcgccacg gctccgccag cggcgccatc tcttttacag
     6421 tcggccgcgt cttaagaccc aatccgatct tcacggcttc ctgcacggcg agatcgcccg
     6481 cgggccatgc atcgccatgg ccgaggcaaa acaacagata gacgtcggcg gtccatgggc
     6541 cgatgccgtg caacgccgtc agcgtgtggt gcgtggcgtc ggcgtcctcg ttcgccagca
     6601 cgtcgaaatt caatctcccg gcggcgagtt cgcgcgcgac gcccttcaag gtcttgatct
     6661 tcgccgccga cagtccgagc ctgcccagac ggtccgcccg ggcgcgacgc aggctgtcgt
     6721 gatcaaacgg atcgaatgcg gcgctgacgc gggcccaaat cgcggcggca ctggcggtag
     6781 agagttgctg gccgctgaca atcgccgcaa ggccggcaaa gccgggttgg cgtcgccgca
     6841 gcgccggcat gcccgcgacc tcaaatatgg gtttgagccg cgggtcctgt tttatcagcg
     6901 cgtggatggc ctgttcgagg tcggcctggg tgttgaggtg gagggtcatt tctcaattct
     6961 tcgtcatggc cgggcttgtc ccggccatcc acgtcttcgt acgatatgcg cgccgaacac
     7021 ggatcatttg tcacactaca gaaagcgatg ggcggattgg ttagaatgca aaagcaaccg
     7081 cctgagcact gttgcaagac gtggatggcc gggacaagcc cggccatgac gagatccgaa
     7141 taatgccgcc gcccgtcttc cgctttgcgc cgagcccgaa cggctatctg catcttggcc
     7201 acgcgctttc ggccctgctg aatttcgatt ccgcgcgccg cagcggcggc cgttttttgc
     7261 tgcgcatcga ggatatcgac gcaacccgct gcaggccgga attcgagacg gcgatctatg
     7321 aagacctcgc ctggctcggc atcgcctggg aaacgccggt gcggcggcag tcagaacatc
     7381 tttctgaata ccgcgacgcg gtcgaaagac tcgctgccga gggtctggtc tatccgagtt
     7441 tcgaaagccg cggcgagatt gcaagactgg tggcgcagcg ggaggccgag gcgccctggc
     7501 cgcgcgatcc ggacggcacg ccgctctatc ccggtgctgc gaaatcgctc ccggccgatc
     7561 aacgcgcgcg attgcttgaa tcgggcgcgc tttatgcgct gcggctggat atggcggcag
     7621 cgcgagcgcg tgccggcgat ctaacctggc tagaacacgg cgaagggccc gatggcgaga
     7681 ccggcagggt ggccgcccgg ccgcaggcct ggggcgacgt catcctggcg cgaaaggagg
     7741 cgccgaccag ctatcatctc tcggtggtga tcgacgacgc ccggcagggc gtgaccgagg
     7801 tggtgcgggg cgaggatctg ttctggtcaa ccagcgtcca ccggctgctg cagcaattgc
     7861 tcggtctgcc gcatcccgtc taccggcacc accgcctcgt ccgcgacggt gaggggcgaa
     7921 aactctcgaa gtcgaccatg gccacgggcc tgcgtgagct gcgcgccgcg ggagcgacac
     7981 cagccgacat ccgccgcggc gtgggtctgc cttaattgcg gcagaactat cccaagaact
     8041 atcccaagag ctatccaaag aaaccatacc aacaactgtc caaagttgaa aattcctcgc
     8101 gcgtgactcc cgcggcccga ccgtggcatg ctggcatcgg ccacggtggg gattcatggc
     8161 gccaaaaccg cgcttaaagc gcccgatgcg gccgcccaaa gggcggctga aaaaacgcgt
     8221 tccggccgtt gccgcggcta acgcgcgcgg caagcgcgcc gccatgattc ctgtcccgag
     8281 tatggtcgaa gcggcgctcg cggcgttcgc gcacgaggtc cgcacgccgc tgaccggaat
     8341 tctggccatc agcaatctgc tcgcgacctc ggacctcgac gagcgcgagc ggcgctgggt
     8401 cgacaccatc aaggcggggg ccgaacacct tgccagtctc gcgaccttgt tcgtcgatgc
     8461 cgcgcgaagc ggcaacgccg cggccgcggt gcggcaggac ttcttcgatc tgcgggcgct
     8521 ggcgcgcagc atcggggatt cgctggccgg cagggcggcg gcgaagggcc tgcaatcctc
     8581 gatcgatatc tcggagaagc ttccagcgcc ggtggtcggc gatccggtcc ggcttcgcgc
     8641 cgcgctggaa aacctgatcg acaatgcggt gaagttcacg gaaaccggtg gtgtcgcgct
     8701 gacggtcaag gcggtgcgcg gccctaaagg caaagtcggc gtcagctttg caatttccga
     8761 cagcggcatc gggctgacgc tcaatgagat caaacgcctg ttccggccgt tctcgcaggc
     8821 caatgtcagc atcgcgtccc gcttcggtgg cgcggggctc ggattatcgt cggtcaagca
     8881 gctcgcgcgc gtcatgggtg gcgacatcgt cgtttcgcaa cggcgcaatg gaggcgccac
     8941 attcaccctg accgtgacct tgagccgcgc aagagcatcg aaggcggccg gtgcggaccg
     9001 ggaaaatgat gcattgtcgg atccggagcg gccgctgcgc gttctcagcg tcgaggacaa
     9061 tccgttcggc cgcgtcgtgc tcaatgcggt cctcaccgaa ctcggtcaca gtgcggaatt
     9121 catcgggcga ggcgaggccg cggccgagcg catcgcgcaa ggcgcgttcg acgcggtgct
     9181 gatggacatg gtgttgccgg gcatcaacgg catcgaggcc atcaagcaaa ttcgcgagct
     9241 ggacgcgccg ctcggcggca ttgcgatcat cggggtgtcc ggccgcggcg acgatgaagc
     9301 cgcatcgcgc gccgccggcg ccgatgcctt tctggtcaag cctgtgagtc caagggcttt
     9361 agcgactgcg ctgcttgaag cgacacgccg tgcggcaacc gcgacttgat gatcgccgcg
     9421 ttcagttcgc cgccatacac gaagatcgcg gcgatgaaat acaggaacac cagcgcgatg
     9481 atgacagacg caaggccggc atacatggtg acgtagttgt tggcgaagcg cgcgagatat
     9541 tgtccgaaca cgatgcccga cagcagcgac gccaccaggg tgaagatgat gccgggcagg
     9601 atctgcagaa acccgcgtcg gcccgccggg agccaggcgt ggagaacaaa cagcgcgacg
     9661 accagcgccg cgatggcgat gccgtagcgg gagaggttga ggaaattctc gttgctttcg
     9721 acgatcagcg gaatgtagcg gcgggccgcc tccagcatca gggggccgag gacgatcagg
     9781 aacgccatcg ccagggcggt gaaggcggcg atcaaggtgt agccgatcga ttccagccgc
     9841 agccagtacc agcgccgcgg ttcgatcacc gaataggcgc ggttaagcgc gacgcgaagg
     9901 ctttcgacgc cattcgatgc aaaatacacc gcgagcacgg cgccgatggt cagcgcgtcg
     9961 ccgcgggtcg tagtcagcac atcgtgaatc tcgcccgaca gcgagtcggc gacctgcttc
    10021 ggccagacct ccagcatcag ccccgctgcc tggtcggcga gttccttcga cccgaaaaag
    10081 ccggccagcg aagtcagcac gatcagaaac ggaaacagcg ccatcagcgt cgacagcgcg
    10141 atatggcttg cgatcgccca gccgtcatcg gcgagaaacg tgtagaacgc gtccatgccg
    10201 acatgaaaga tgtagcgaag ctgcctcaca tttcaccttg accattggaa gatgcgttcg
    10261 atcccgcgaa ccgggctacc ttctgatatt attgagctaa aagccagatc gttgcatcgg
    10321 agc
//
LOCUS       DASWGM010000644         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1869752,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000644 DASWGM010000000
VERSION     DASWGM010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869752"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VGA15_18125"
     CDS             complement(<1..708)
                     /locus_tag="VGA15_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9589647.1"
                     /translation="MPTSFNFADPRLSKLDGFQVEIDETRERADIILNRPPYNIVSMP
                     QRDQLRLVFETLDEDPRVRVIVVRSIGEHFSSGGNIGGFMEATPEHVSKLAWNIASPA
                     RCAKPVIVANRGYCFGVGFELSLACDFRIASETTFYALPEQKLGQIPGSGGSARLQKM
                     IGITRTKDIVMRSKRIAGKQAQEWGIATECVPDAELEKATDKLVDELRTFSPLAQRTA
                     KKLLNDTEDSTLAIAIEL"
     gene            complement(740..2293)
                     /locus_tag="VGA15_18130"
     CDS             complement(740..2293)
                     /locus_tag="VGA15_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9589648.1"
                     /translation="MFDLGTSFVASVARDPGAVAIVDAGLRLTYAQWFTRISSVVAAF
                     DDLGLRPGDHIVTVLQNRWEAATIHWACQLAGLVITPINWRAKSDELDFCVENSQARA
                     LVYESVSAGAVAGATFARNLRRIVVGDAVAADDQAVLFATMIDTSAPETAPRAGPEAW
                     SIMLYTSGTTSKPKGVPRRHRAERAAGVAHVAQNLYGRYERTLGVMPLYHTMGVRSLI
                     SMSLIGGTFVCLPRYDPGLALAIIAAEKITNLYLVPTLYHDLVHHSDFEKTNTSSIRK
                     LGFAGAPMTDGLLQKLNSAFKPDLFVNHYGSSEIYTFTVNQNAVAKPGSAGKAGINQF
                     VRVVKLAARSASDLASTEEEGEIIALLAGDESFEGYWRRPDADAKSLRGGWYFTGDTG
                     FVDKDGDLFVTGRTDDMIITGGENVSPVEIESCLSLHPLVSEVAVVGLADERWGKIVA
                     AFVKRSGYVSEADLEAFCRDSGLANFKRPRKFVFVDAIPKSPVGKLLRRLLVAGEYDV
                     ERRPPSTAV"
     gene            complement(2286..2810)
                     /locus_tag="VGA15_18135"
     CDS             complement(2286..2810)
                     /locus_tag="VGA15_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9589649.1"
                     /translation="MTRLESDRRHRIRFTLNGAAAHGEAEPRMLLTDFLRHQIGATGT
                     HVGCEHGVCGACTVTVDGMIARACLTLAVQIDGSDVRTVEGLAPAPDRLGILQAAFKR
                     HHALQCGFCTAGILMSLDHYLREREQPTEAELRDFLSGHLCRCTGYTPIIRAALDAAD
                     LLANQPRAKMKTDV"
     gene            complement(2807..3622)
                     /locus_tag="VGA15_18140"
     CDS             complement(2807..3622)
                     /locus_tag="VGA15_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9589650.1"
                     /translation="MKPAPFDYVRAETLDEALAVLASEGSDAQIMAGGQSLMAMLNTR
                     LAKPKVLVDIMRLKELDRIDATAAAVTVGAGVRQRSLLGWPPLAEKLRLVALALPWTG
                     HAQTRSRGTICGSIAHADPSAEMPLVLLALGGEVHLRTASKRRRVAAEKFFTGMMSTA
                     RAADEMIEAVSFPAAGVARCAFREVARRHGDFAIVACAAVASRQGVRFAVGGVADMAA
                     VRDLPRLDGSALDDALNAFAHELDARDDVHATARYRRDLVRLIGRDLVREVLQ"
     gene            complement(3619..>4266)
                     /locus_tag="VGA15_18145"
     CDS             complement(3619..>4266)
                     /locus_tag="VGA15_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX9589651.1"
                     /translation="NAVADALGVKDVELPLVPAKLADIVRGAEPPPPAGRRADMPKAA
                     NGGRKLRGEGSATVAASPEAVWTMLLDPATLQAVIPGCHGVEKISDTHFHAEVTIGVG
                     PVKGRYRAEVTLSDLDAPRAVTLGGFAEGALGFGNGEGRITLTPEHAGTVVRYEYDAA
                     IGGKVASIGGRLLDGAARVIIGQFFSGLARQAGGASAQDRPSLLRKLLSLFGRRP"
BASE COUNT          779 a         1372 c         1359 g          756 t
ORIGIN      
        1 gagctcgatc gcaatcgcga gcgtcgagtc ctcggtgtcg ttcaagagct ttttggcggt
       61 tcgctgagcc agcggcgaga acgtgcgaag ttcgtcgaca agcttgtcgg tcgctttctc
      121 caattcggca tcgggcacgc actcggtggc gatgccccat tcctgggcct gtttgccggc
      181 aatccgcttc gaacgcatca cgatgtcctt ggtgcgcgtg atgccgatca tcttctgcag
      241 ccgcgcggat ccgccagagc ccggaatctg tccgagcttc tgctccggca acgcgtagaa
      301 tgtggtctcc gacgcgattc tgaagtcgca ggccaaagac agttcgaacc cgacgccgaa
      361 gcaatagccg cgattggcga cgatcaccgg cttggcgcag cgcgcaggcg aggcgatatt
      421 ccaggcaagc ttcgacacgt gctccggcgt ggcttccatg aagcctccga tattgccgcc
      481 gctggagaaa tgctcgccga tcgatcgcac cacgatgacc cggacccgcg gatcctcgtc
      541 gagggtttcg aacaccagcc gaagctgatc gcgctgaggc atcgatacga tgttgtaggg
      601 cggccggttg agaatgatat cggcacgttc gcgagtttcg tcgatttcca cctgaaagcc
      661 gtcgagcttt gacagccgtg gatcggcgaa attgaacgat gttggcattg tggctttcct
      721 tcttcttgaa actcggaacc tatacggcgg tcgatggcgg ccggcgctcg acgtcatatt
      781 cccccgcgac cagcagccgc cggagcagct ttcctaccgg cgatttcggg atcgcatcga
      841 cgaagacaaa ttttcgcgga cgcttgaagt tcgctaaccc ggaatcccgg cagaatgctt
      901 cgagatcggc ctcgctgaca tacccgctgc gcttgacgaa cgcggcgacg atctttcccc
      961 atctctcgtc ggcgagaccg acgaccgcga cctccgacac cagcgggtgc agcgacagac
     1021 agctttcgat ctccaccggc gatacgttct cgccgcctgt gatgatcatg tcgtcggtcc
     1081 ggcccgtgac gaacaggtcg ccatccttgt ccacgaagcc ggtatcgccg gtgaaatacc
     1141 atcccccgcg cagcgacttg gcgtcggcat cgggacgcct ccaatagcct tcgaatgact
     1201 cgtcgccagc cagcagcgcg atgatctcgc cctcctcttc agtcgacgca aggtccgatg
     1261 ccgaccgtgc ggcaagcttg acgaccctga cgaactggtt gatcccggct ttgccggctg
     1321 aaccgggctt cgcgaccgcg ttttgattga ccgtgaaggt gtagatttcc gagcttccgt
     1381 agtgattgac gaacagatcc ggcttgaacg ccgaattcag cttctgcagc agaccgtcgg
     1441 tcatcggcgc gccggcaaag ccgagcttcc gtatgctgga tgtattggtc ttttcgaagt
     1501 ccgaatgatg gaccagatcg tggtaaagcg ttggaaccag atagagattt gtgatttttt
     1561 cggccgcaat gatggcgagt gccaggccgg gatcgtagcg gggaagacac acaaacgtgc
     1621 cgccgatcag cgacatcgag atcagggagc gtacgcccat ggtgtgatag agcggcatga
     1681 cgccaagggt gcgctcgtag cggccgtaca ggttttgcgc cacatgagcg acgccggcgg
     1741 ctcgctcggc gcggtgacgg cggggcacgc ctttcggctt cgacgtcgta cccgacgtat
     1801 agagcatgat cgaccacgcc tcagggcctg cacgcggtgc tgtttccggc gcggacgtat
     1861 cgatcatggt tgcaaacaga acggcctggt cgtcggctgc gacggcgtcg ccgacaacga
     1921 tgcggcgcaa attgcgtgcg aaggtggcgc cggcgaccgc gcctgcggat acgctttcgt
     1981 agacgagagc cctcgcttgc gaattctcga cgcagaaatc gagctcgtcg gatttcgccc
     2041 gccagttgat cggcgtgatg actaatccgg caagctggca ggcccagtga atggtcgcgg
     2101 cttcccatcg gttctgcagc acggtgacga tgtggtcgcc cggcctcaaa ccgagatcgt
     2161 cgaatgcggc gacgacggaa gatatccggg taaaccattg cgcgtaggtc agccgcaggc
     2221 cggcgtccac gatcgcgacg gcgcctggat cgcgtgccac actggcgacg aaactggtac
     2281 cgagatcaaa catctgtttt catttttgca cgaggttggt ttgccagcaa gtcggcggcg
     2341 tcgagagcgg cgcggatgat cggggtgtaa ccggtgcacc ggcacagatg gccgctcagg
     2401 aaatcgcgca actccgcctc agtgggctgt tcccgttcac gaaggtaatg atcgagcgac
     2461 atcagaatgc ccgcggtgca aaagccgcat tgcagcgcgt gatggcgctt gaatgccgcc
     2521 tgcaggatgc ccaatcgatc gggagcgggc gcgaggccct ccacggtgcg gacgtcgctg
     2581 ccgtcgatct gcaccgccag cgtgaggcag gcgcgggcga tcattccgtc cacggtcacg
     2641 gtgcaggccc cgcaaacacc gtgctcgcat ccgacatgcg tgccggtggc gccgatctgg
     2701 tgacgcagaa aatccgtcag cagcatgcgc ggttcggctt cgccatgggc agccgcgccg
     2761 ttcaacgtga acctgatccg gtgccgccgg tccgactcaa ggcgcgtcat tgcagcacct
     2821 cgcggacgag atcgcgaccg atcagccgga ccagatcgcg gcgataacgg gcggtcgcat
     2881 ggacatcgtc ccgcgcatcc agttcatggg cgaaagcgtt cagggcgtcg tcgagcgcac
     2941 tgccatcgag acgcggcaag tcgcgcaccg cggccatatc cgcaaccccg ccgacggcga
     3001 agcgaacgcc ttgcctgctg gcgactgccg cgcaggcgac gatcgcaaag tcgccgtgac
     3061 gccgcgcgac ctcccggaac gcgcagcgcg caacgccagc ggccggaaac gaaaccgcct
     3121 cgatcatttc gtcggcggcg cgggcggtgg acatcatgcc ggtgaagaat ttttcggccg
     3181 cgaccctgcg gcgcttgctg gcggttcgca ggtgcacctc gccgccaagc gccagcaaga
     3241 ccagcggcat ttccgcgctg ggatcggcgt gggcaatcga accgcaaatc gtcccgcggc
     3301 tgcgggtctg ggcgtgcccg gtccagggca gagcgagcgc gacaagcctc agtttttcgg
     3361 caagcggcgg ccagcccagc agtgaccgct gccggacgcc ggcgccgacc gtcacggcgg
     3421 cagccgtcgc gtcgatccgg tccaattcct tcagccgcat gatgtcgacc aggactttgg
     3481 gcttcgccag gcgggtgttc aacatcgcca tcagcgactg gccgcccgcc atgatttgcg
     3541 cgtcgcttcc ttccgaggcc agaaccgcaa gggcctcgtc aagggtttcc gcgcgcacat
     3601 agtcgaaggg agcaggcttc acggccgcct cccgaacaac gacaaaagct tgcgcagcag
     3661 cgacggccgg tcttgcgccg acgcgccgcc ggcctgacgg gccaggcctg aaaagaactg
     3721 gccgatgatg acacgcgccg cgccgtccaa gagacggccg ccgatgctcg cgaccttgcc
     3781 cccgatcgcc gcgtcatatt cgtaacgcac caccgtgccg gcatgctccg gcgtcagcgt
     3841 gatacggcct tcgccattgc cgaacccgag cgctccttcg gcaaaaccgc ccaaggtcac
     3901 ggcgcgcggc gcatcgagat cggacagggt cacttccgcg cgatagcggc ccttgaccgg
     3961 ccctacgccg atcgtgacct cggcgtgaaa atgcgtgtca gaaattttct ccacgccgtg
     4021 gcaacccggg atgaccgctt gaagcgttgc cggatcgagc agcatggtcc acaccgcctc
     4081 gggcgatgcg gccaccgtcg cgctgccctc gccgcggagc ttgcggccgc cgttggcggc
     4141 tttcggcata tcggcacgac gcccggcggg cggcggcggt tcggcgccgc ggacgatgtc
     4201 ggccagtttg gcgggaacca gcggaagctc gacatccttg acgccgagcg cgtcggcgac
     4261 ggcgtt
//
LOCUS       DASWGM010000645         5383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_189477,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000645 DASWGM010000000
VERSION     DASWGM010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189477"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2215
                     /locus_tag="VGA15_18150"
     CDS             <1..2215
                     /locus_tag="VGA15_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX9589652.1"
                     /translation="GIQRIKIDDSLSQLFRSNSKEYRQYEAETKRFPATEFDVLVVVE
                     GKTLLARDNLERVRELVTDLQLVEGTRGLISLFSARQAPAPGKLPAALFASELPQGAD
                     YDKFIETVKTNEIIRGKLLSEDGTLALIVLSLDPDVVASSKLSKTIGDIRKLMAEDLS
                     GSRLSAELSGVPVMQLEIRNAVERDGLTYNILGILAGCIIAIIFFRKISFMVVAAFPP
                     MIAILLALGGLGWANFNLNMFLNVMTPLIMVISFSDSMQLTFAARDRLIAGQDKYTAF
                     RNAVLVVGPACVLTHGTAGISFIALQFSDSDLIRKFGEAGLAATIIALIAVLSLVPVF
                     GVLFVRNEKVFAAKFQGADAGVNALRAFCYWIAVRMVSRPGLFSLIAVIFVGGLAVVY
                     ANLEPRYRLADQVPDKQQAVAASGRLDAKLTGANPIDVLIEFPKGTSLYAPQTLQIIA
                     DVHSLVEKQAGVGNVWSLETLRRWLAEKAGSSDVATLQEYVSVIPAHLVRRFISADQD
                     AVVVSGRVPDLDSSQILPVVENLDHALDTVRKDHPGYEIAVTGLSAIAARNSASMIEK
                     LNRGLTIEFALVAVFIGLAFRSVVVMLSCILPGIFPVVLSGTVLYALGEGLQFASVVA
                     LTVSFGLGLSATIHFLNRLRLETEPGVSPELAVERATVLVGPALILTTVVLACGLVVT
                     VFSDLPSLRLFGWLSAFAMVAALIADLFILRPTAMFLINLERRLRGTAGRAKPAE"
     gene            complement(2242..2745)
                     /locus_tag="VGA15_18155"
     CDS             complement(2242..2745)
                     /locus_tag="VGA15_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589653.1"
                     /translation="MKLFGSSLVGQAIKAAGIGAALMLSVSAGHAQAGPFAGFEGAWS
                     GNGTVALSDGTTERIRCKADYKVNGSGLGLKQSLRCASDSYKFELSSDVTSQGDRISG
                     NWSEASRNIFGNLQGTAGGGQIDVFVEASGFAANLTLRTMGNRQSVQINSKGEIRGVN
                     ITMTKGG"
     gene            3286..3525
                     /locus_tag="VGA15_18160"
     CDS             3286..3525
                     /locus_tag="VGA15_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589654.1"
                     /translation="MRNFFLALTLFSISVSTGAIAQQQQRSGTPEEQKACSRDVERHC
                     RPVIDQGDFTILACLQQNRPKLSAACAQVLKDHNQ"
     gene            3721..4599
                     /gene="hpnC"
                     /locus_tag="VGA15_18165"
     CDS             3721..4599
                     /gene="hpnC"
                     /locus_tag="VGA15_18165"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX9589655.1"
                     /translation="MTSASELRSGKTHRDENFPVASWIIHPRHRALILAFYNFVRTAD
                     DIADHATLAERDKLNYLDLLEAELLGQGDSQPEAVNLRAALAQRSMPPRHALDVLIAF
                     RMDVTKLRYENWDEVIHYCRYSAMPVGRFMLDVHGESTSTWAASDALCAGLQINNHLQ
                     DCGKDYRDLNRVYLPRDALAAAGASVEALGQKQASAPLLQCLHSLAARTEILLNESKP
                     LSAEVRDLRLGLEISVIQAFADKIVRLLKVRDPLSQTVHLGPTELIAHSLGGLAGEMA
                     RRAVGRRPVSKPAAGA"
     gene            4596..>5383
                     /gene="hpnD"
                     /locus_tag="VGA15_18170"
     CDS             4596..>5383
                     /gene="hpnD"
                     /locus_tag="VGA15_18170"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX9589656.1"
                     /translation="MTLQTAAANASYGTTASGSSFYAAMRILPREQREAMFQIYSFCR
                     QVDDIADSDGPRPERLAALQQWRDDIDALYQGHPPARLQAYVASVRTFDLKREDFLAI
                     VDGMEMDVPQDIRAPDLATLDLYCDRVASAVGRLSVRVFGLARDDGILLAHHLGRALQ
                     LTNILRDVDEDAAIGRLYLPREGLLHAGITSFDPLEVISSPALPKVCAPLVERARMHF
                     EKADEVMARNPRRVVRAPKIMSKYYRAILELLVARGFASPRMPVR"
BASE COUNT          965 a         1638 c         1651 g         1129 t
ORIGIN      
        1 cggtatccag cgcatcaaga tcgacgattc cctcagtcag ttgttccggt ccaattccaa
       61 ggaataccgg caatatgaag cggaaaccaa gcgcttcccg gcgacggaat tcgacgtgct
      121 ggtcgtggtc gaaggcaaga cgctgctggc gcgcgacaat ctcgaaaggg tgcgcgaact
      181 ggttaccgat ctgcaactcg tcgagggcac gcgcggcctg atttcgctgt tctcggcgcg
      241 gcaggccccg gcgccaggca agctgccggc ggcactgttc gcgtcggagc tgccgcaagg
      301 cgcggactac gacaagttca tcgagaccgt taaaaccaac gagatcatcc gcggcaagct
      361 gttgtccgaa gacggcacgc tggcgctgat cgtgctgtcg ctcgatcccg atgtcgtcgc
      421 cagcagcaag ctcagcaaga cgatcggcga cattcgcaag ctgatggcgg aggatctctc
      481 gggtagcagg ctcagcgccg agctatcggg cgttcccgtc atgcagctcg aaatccgcaa
      541 cgcggtcgag cgcgatgggc tgacctacaa catcctgggt attctggccg gctgcatcat
      601 cgcgatcatc ttcttccgca agatatcgtt catggtcgtc gccgccttcc cgccgatgat
      661 cgcgatcttg ctggcgctcg gcggcctcgg atgggccaat ttcaacctca atatgttttt
      721 gaacgtgatg acgccgctga tcatggtcat cagcttttcg gactcgatgc agctgacctt
      781 cgccgcgcga gaccgtctga tcgcgggcca ggacaaatac accgcgttca ggaacgccgt
      841 gctggtggtc gggccggcct gcgtgctcac ccacggcacc gccggcattt cgtttattgc
      901 cctgcaattt tcggattccg acctgatccg caaattcggc gaggcggggc ttgccgccac
      961 catcatcgcg ctgatcgccg tgctttcgct ggtgccggtg ttcggggtgc tgtttgttcg
     1021 caacgaaaag gttttcgccg cgaaattcca gggcgccgac gccggcgtca acgcgctgcg
     1081 ggctttctgc tactggatag cggtgcgcat ggtcagccgc cccgggctgt tcagcctgat
     1141 cgcggtgatt tttgttggcg gtttggcggt cgtctatgcc aacctggagc cgcgttaccg
     1201 gctcgccgat caggtgccgg acaagcagca ggcggtggcg gccagcgggc gtctcgacgc
     1261 caagctgaca ggtgccaatc cgatcgacgt cctgatcgaa tttcccaagg gcacctcgct
     1321 ctatgctccc cagacgctgc agatcatcgc cgacgttcac tcgctggtcg agaaacaggc
     1381 cggcgtcggc aatgtctggt cgctggagac gctgcgccgc tggcttgcgg aaaaagccgg
     1441 aagctccgac gtcgcgacat tgcaggaata tgtcagcgtg attcctgcgc atctggtccg
     1501 gcgtttcatt tccgccgatc aggatgcggt ggtggtgtcg ggccgggttc ccgatctgga
     1561 ttcaagccag attcttcccg tggtcgaaaa tctcgatcat gcgctggata cggtgcgcaa
     1621 ggaccatccc ggctacgaga ttgccgtcac cggtctgtcg gcgatcgctg cgcgcaacag
     1681 cgccagcatg atcgaaaagc tcaatcgcgg gctgaccatc gagtttgcat tggtcgcggt
     1741 cttcatcggg ctggcgttcc gctcggtggt cgtgatgctg tcctgcatcc tgccgggcat
     1801 tttcccggtg gtgctttccg gcacggtact ctacgcgctg ggggagggac tgcaattcgc
     1861 gagcgtggtt gcgctcacgg tgtcgtttgg cctcggtctc agcgccacga tccacttcct
     1921 caaccggttg cggctggaga ccgagccggg cgtcagtccg gaactggcgg tcgagcgcgc
     1981 gaccgtgctg gtcggtccgg cgctgatcct caccacggtg gtgctggcct gcggtctggt
     2041 ggtgacggtg ttttccgacc tgccgtcgct gcgcctgttt ggctggctca gcgcgtttgc
     2101 aatggttgcg gccctgatag ccgacctgtt tatcctgcgg ccgacggcga tgttcctgat
     2161 caatctcgag cgacggcttc gcgggacggc aggccgtgcg aagccggcgg agtagacaaa
     2221 ggcgcgaggg agggtgcggc gtcagccgcc cttcgtcatc gtgatattca cgcccctgat
     2281 ttcgcccttg gagttgatct gcacgctttg ccggttaccc atcgtgcgca acgtcaggtt
     2341 ggcggcaaat cccgaagcct cgacgaacac gtcgatctga ccgccgcccg ccgtgccctg
     2401 cagattgccg aaaatattgc ggctggcttc gctccagttg ccggaaatgc ggtcgccctg
     2461 gctcgtgaca tcgctgctca gttcgaattt gtagctatcg ctggcgcagc ggagagattg
     2521 tttcagaccc agtccggagc cgttgacctt gtaatcggct ttgcaccgga tgcgctcggt
     2581 cgttccgtcc gacagcgcaa cggttccgtt gccagaccac gcgccttcaa aaccggcaaa
     2641 cgggccagcc tgggcgtggc ccgccgacac cgacaacatc agcgcggcgc cgataccggc
     2701 agccttgatc gcctgtccaa ccaagctgga accaaacaat ttcatatcgc gttttcccat
     2761 tcaaattgac gttctcatac cagatttctc atacgagata cgtctcgccg catcgaagat
     2821 ttagtgtcgc cgcagtgtgt taggttcaaa tgacaaaatc gaccgctgcc cgcaattacg
     2881 ccggccggcc ttggccgtgg gaccggatat cgcaaaatac ccgaatcgat tgacgccagg
     2941 cgaacggcat ttttcggtca gcgaaatcaa aaaagtttcg gatatcgcca cgttttatct
     3001 ggcagcatct agctgttggc cggcttcgcc tgcaatagtc aatttcaaaa aacgccaatc
     3061 aataaataag tcttttgtat caaacgttta ttggcgttgt cgggtttgcc tgcaagtgtg
     3121 ctgcatttgc atcggatcgc cgcaaatttg gccgcattta gcagcgcttc tggttcgcgc
     3181 gttgcttggg ttcgcgacgt ttgcaatcta aacaaatctg tcctggtcat gcctcgacgc
     3241 tagtcgggat cgaaattttc tctggcttca gaatgaagga acacgatgcg taattttttc
     3301 ctggcactta ccctgttttc gatctcggtt tccaccggtg ccatcgccca gcagcagcag
     3361 cgcagcggca cgccggagga gcagaaggcc tgttcgcgcg atgtcgagcg ccactgccgc
     3421 ccggtgatcg atcagggcga cttcaccatt ctggcttgcc tgcagcaaaa tcgtccgaag
     3481 ttgagcgcgg catgcgcgca ggtgctgaaa gaccacaacc aataggacag gactcgcatc
     3541 cgacaattgc cgctgccggc cgattgatgg cctaattcgg acagtcggag tgtgcgagaa
     3601 ctcacagatt gcgttggttt tggcgtcgta ttcccctata tggggttcgc gatcgttccg
     3661 catgcgcgga ttaccgggca tgcgaggtcg ccatgcccag accaatgtgg caagttcttg
     3721 atgaccagtg cgagcgaact gcgatccggc aagactcacc gcgacgaaaa ttttccggtc
     3781 gcgtcatgga tcattcatcc gcgccaccgg gcgctgattc ttgcgttcta caatttcgtc
     3841 agaaccgccg acgacatcgc cgatcatgca acactcgctg agcgcgacaa gctcaactat
     3901 ctcgatctgc tcgaagccga actgctgggg cagggcgaca gccagcccga ggcggtcaat
     3961 ctgcgggccg ccctggcgca gcgttcgatg ccgccgcgcc acgccctcga cgttctgatc
     4021 gcatttcgta tggatgtgac caagcttcga tatgaaaact gggacgaagt gatccactat
     4081 tgccgatact cggcgatgcc ggtcggacgg ttcatgctcg acgtccacgg cgagagcacg
     4141 tcgacctggg ccgcctcgga cgcgctctgc gccggcctgc aaatcaacaa tcatctgcag
     4201 gactgcggca aagactatcg agacctcaat cgggtttatc ttccgcgcga tgcgctggcg
     4261 gcagcggggg cctccgtcga ggcgctcgga cagaagcaag catcggcgcc gctgctgcaa
     4321 tgcctgcatt cgcttgcggc gcggaccgag attctgctca acgaaagcaa gccgctcagc
     4381 gccgaggtgc gggatttgcg gctcgggctc gagatctccg tgatccaggc gttcgccgac
     4441 aagatcgtcc gtcttttgaa ggtgcgcgat cccttgagcc agaccgtgca tctcggcccc
     4501 actgaactga tcgcccacag cctcggcggc ttggcaggcg agatggcccg ccgggcggtg
     4561 ggacggcggc ccgtgtccaa accggcagcc ggcgcatgac gcttcagacg gcggcggcca
     4621 acgctagcta cgggaccacc gcgtccggca gttcgtttta tgcggcgatg cgcattttgc
     4681 cgcgcgagca gcgcgaggcg atgttccaga tctacagttt ttgccgccag gtcgatgaca
     4741 tcgccgattc cgatgggccg cggcccgaac ggctggccgc gctgcagcaa tggcgtgacg
     4801 atatcgacgc actctatcag ggccacccgc cggcgcgatt gcaggcctac gtggcctcgg
     4861 ttcgaacgtt cgatctcaag cgcgaggatt ttctggccat cgtcgatggc atggagatgg
     4921 atgtgccgca agatatccgg gcgccggatc ttgcgacgct cgatctgtat tgcgaccgcg
     4981 tcgccagcgc ggtcgggcgc ttgtcggtgc gggtgttcgg tctggcgcgg gacgacggca
     5041 tcctgctcgc gcaccatctt ggccgcgcgc tgcaattgac gaatatcctg cgcgacgtcg
     5101 acgaagacgc cgcgatcggc cggctttatc tgccgcgcga aggtctgctg cacgccggaa
     5161 tcaccagctt cgatccgctc gaggtgatct ccagcccggc gttgccaaaa gtatgcgcgc
     5221 cgctggtcga gcgggcgcgg atgcatttcg agaaggccga cgaagtcatg gcccgcaatc
     5281 cgcggcgcgt ggtgcgcgcg ccgaagatca tgtcgaaata ttatcgggcg atcctggagc
     5341 tgttggtcgc cagggggttt gcatcgccgc gcatgccggt tcg
//
LOCUS       DASWGM010000646         4648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1749084,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000646 DASWGM010000000
VERSION     DASWGM010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749084"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VGA15_18175"
     CDS             <1..495
                     /locus_tag="VGA15_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9589657.1"
                     /translation="PVANRLGDEGIGFKIAMAGLDGGRINIAACSLGGAQCALDKSLA
                     YMKERKAFGKRLDEFQALQFRLADMATELEAARTFVWRAAAALDRKDADATMLCAMAK
                     RFGTDVGFEVANQALQLHGGYGYLSEYGIEKIVRDLRVHQILEGTNEIMRLIVSRKLI
                     EGAR"
     gene            492..1580
                     /locus_tag="VGA15_18180"
     CDS             492..1580
                     /locus_tag="VGA15_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9589658.1"
                     /translation="MTAQAAAVAEPDLIARREGAVGVIRLNRPKSINAVTLRMFRDID
                     KALDAFEADPAVSAILLEGAGERGLCAGGDIRALYESSKVHGDLGRVLWREEYILNAR
                     IAKFPKPYVAFMDGIVMGGGVGLSAHSRHRVVTERTKLAMPEVGLGFFPDVGGTWLLS
                     RSPGEIGTYFGLTGQTMNGPDAIHARFADAVVPSSKLAALREALTRVRAGATSDEIKT
                     IIDGFATGETSGPVAAMQSHIDGWFAHDRMQDIVAALQRDGSELAQSTLKTLNEKSPR
                     GMVVTLKLLRLARQSSSLEECLVREYRAALQVFASDDFREGVRAAVIDKDRNPKWSPP
                     GIEDVTPEMVAPYFAAIGADELVFDKTK"
     gene            1608..2495
                     /gene="mmsB"
                     /locus_tag="VGA15_18185"
     CDS             1608..2495
                     /gene="mmsB"
                     /locus_tag="VGA15_18185"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEX9589659.1"
                     /translation="MASIAFIGLGNMGGPMAANLVKAGDKVIAFDLVEASRNQAKADG
                     AAIADSAAAAVKGADTVITMLPAGKHVLSVWNEVVPSMAKGALIIDCSTIDVESAVAS
                     HALAAKQGIASVDAPVSGGTGGAKGATLTFMAGGEDKAFAAAKPVLEKMGKKIVHCGG
                     AGAGQAAKICNNMILGISMIAVSEAFALAEKLGLSHQALFDVASTSSGQCWSLTSYCP
                     VPGPVPTSPANNDYRPGFASALMVKDLTLAQDAANAAGAATPLGKHAQEIYKAFDAAG
                     NGGVDFSGIIQHVRGLASK"
     gene            2667..4403
                     /locus_tag="VGA15_18190"
     CDS             2667..4403
                     /locus_tag="VGA15_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9589660.1"
                     /translation="MTTFQEARAFLLKHRSDYDTAVAEFRWPDPVPFNWALDWFDAEL
                     ARNLQSKDRAALWIVDAGSNRETKLSFEALSRRSNQVANFLRAQGLKRGDHLLLLLGN
                     VIPLWETMLAAMKLGVVVIPATTLLTPDELRDRLDRGRARVVVASQDQVAKFAGLGGD
                     HLVRIVVSASSKHEGWLAYEQAADFPQTFEPDGPTHADDPMLLYFTSGTTAKPKLVRH
                     SQRSYPVGGLSTMYWLGLQPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRF
                     DAKGLLATIGRCGVTTLCAPPTVWRLFIQEKLATFKVSLREVCGAGEPLNPEVIDQVR
                     AAWGLTIRDGYGQTETTALAGNSPGQKVKVGSMGRPLPGYQVQITDIDGQVTNEGEVS
                     LVLGADWPAGLMQGYQRDDGTLSGADGDLYRSGDVVFADDEGYLTFVGRSDDVFKSSD
                     YRISPFELESILLEHEAVAEAAVVPSPDPIRLAIPKAYVLLVSGVARSPATALSIFAH
                     LHTRLAPFKRIRRIELVTELPKTISGKIRRVQLRRLEHDNDRDDPLRGQEFREEEFPE
                     LQTLRSAGSPEN"
     gene            4407..>4648
                     /locus_tag="VGA15_18195"
     CDS             4407..>4648
                     /locus_tag="VGA15_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX9589661.1"
                     /translation="MNEVWKKPPVSLEAYQNMVGQEVGVSSWHLVDQNRIDLYADVIE
                     DHQFIHVDPERAKRETSYGTTVAHGFLTMSLMSIMS"
BASE COUNT          868 a         1375 c         1560 g          845 t
ORIGIN      
        1 ccggtcgcca atcgcctcgg cgacgagggc atcggcttca agatcgcgat ggccgggctc
       61 gacggcggcc gcatcaatat cgcggcctgt tcgctcggcg gggcgcaatg cgcgctcgac
      121 aagtcgctgg cctacatgaa agagcgcaag gcttttggaa agcgcctcga tgaattccag
      181 gcactgcaat ttcgtctggc cgacatggcg accgaactgg aggcggcgcg gacctttgtg
      241 tggcgcgcgg cggcagcgct cgaccgcaag gatgccgacg ccaccatgtt gtgcgcgatg
      301 gcaaaacgct tcggcaccga cgttggcttc gaggtcgcca atcaggcgct gcaactgcat
      361 ggcggttacg gctacctcag cgaatacggc atcgagaaaa tcgtgcgcga tctgcgcgtg
      421 caccagattc tggaaggcac caacgaaatc atgcggctga tcgtttcacg caagctgatc
      481 gagggcgcgc gatgacggct caagctgccg ctgttgccga acccgacctg atcgcgcggc
      541 gcgagggcgc tgtgggagtt atcaggctca accggccgaa atcgatcaat gcggtgacgc
      601 ttcggatgtt tcgcgacatc gacaaggcgc tcgatgcgtt cgaggctgat ccggcggtgt
      661 cggcgatcct gctggaaggc gccggcgaac gcggcctgtg cgccggcggc gatatccgcg
      721 cactgtatga aagctcaaag gttcacggcg atctcggcag ggtcctgtgg cgcgaggagt
      781 acattctcaa cgcgcgcatc gccaaatttc caaagcccta tgtcgccttc atggacggca
      841 tcgtgatggg cggcggcgtc ggtctgtccg cgcacagccg tcaccgcgtc gtcaccgagc
      901 gcaccaaact tgcgatgccg gaagtcgggc tcggcttctt tcccgatgtc ggcggcacct
      961 ggctgctgtc gcggtcaccg ggcgagatcg gcacctattt tggattgacc ggccagacca
     1021 tgaacggccc cgacgcgatc catgcccgct ttgccgacgc ggtcgtgcca tccagtaaat
     1081 tggctgcctt gcgcgaggcg ctcaccaggg ttcgcgccgg tgcgacgtcc gacgaaatca
     1141 agaccatcat cgatgggttt gcgaccggcg agacgtcagg acccgtggcg gcgatgcagt
     1201 cgcatatcga cggctggttt gcccatgacc gaatgcagga tatcgtcgca gccctgcagc
     1261 gggatggttc ggagctcgcg cagtcgacac tgaagacatt gaacgagaag tcgccgcgcg
     1321 gcatggtggt caccttgaaa ctgctgcggc tggcgcgtca atcgtcctcg ctggaagaat
     1381 gtctggtgcg ggaatatcgt gccgcgctgc aagtgttcgc cagcgacgat tttcgcgaag
     1441 gcgtgcgggc ggccgtgatc gacaaggacc gcaatccaaa atggtcgccg ccaggaatcg
     1501 aggatgtgac gccggagatg gtcgcgcctt acttcgcggc aatcggcgcc gacgagctgg
     1561 tgttcgataa aactaaatag agacgccttg agcggagggg actagacatg gcaagcatcg
     1621 cattcatcgg cctcggcaac atgggtggcc cgatggcggc caatctggtc aaggccggcg
     1681 acaaggtgat cgcgttcgat ctggtggagg cttcgcgcaa ccaggcgaaa gcggatgggg
     1741 ctgcgatcgc cgacagcgct gcggccgcgg tgaagggcgc cgacacggtc atcaccatgc
     1801 tgcccgcggg caagcatgtc ctctcggtgt ggaacgaagt cgttccgtca atggcaaaag
     1861 gcgcgctgat catcgattgt tcgaccatcg acgtcgaaag cgcggtggct tcccatgcgc
     1921 tggcggcgaa acagggcatc gcctcggtcg atgcgccggt gtcgggcggc accggcggtg
     1981 ccaagggcgc gacattgacc ttcatggccg gcggcgaaga caaagcgttt gcagcggcga
     2041 agcccgtgct ggaaaaaatg ggcaagaaga tcgtgcattg cggcggcgcc ggcgctggcc
     2101 aggcggcgaa aatctgcaac aacatgattc tgggaatttc catgatcgcg gtcagcgaag
     2161 cctttgcgct cgccgaaaaa ctcgggttgt cgcatcaggc gctgttcgac gtcgcctcga
     2221 cctcgtcggg acaatgctgg tcgctgacat cctattgtcc ggtgccgggc ccggtgccga
     2281 cgtcgcctgc gaacaacgac tacagaccgg gctttgcctc ggcgttgatg gtgaaggacc
     2341 tcacattggc gcaggatgcg gccaacgccg cgggtgctgc aacgccgctc ggaaaacacg
     2401 cgcaggaaat ctacaaggcg ttcgatgccg cgggaaatgg cggggtggat ttctcgggga
     2461 ttattcagca cgtccgcggg ttggcgagca agtgatggcc gtcatccccg cgcaacggct
     2521 ttgccgttgt cgctggaggt gcgagcgaag cgagcctcga aggatgagga gttcagtgcg
     2581 tgtggcccat ccttcgagac gccgcgcaag ggcacggctc ctcaggatga cggttgtggg
     2641 cgcggcgaaa gcggttaaga tttgacatga cgaccttcca ggaagcgcgc gcattcttgc
     2701 tcaaacatcg ttccgactac gataccgccg tcgcggagtt ccgctggccg gatccggtgc
     2761 cgttcaactg ggcgctggac tggttcgacg cggagctggc gcgcaatctg cagagcaagg
     2821 accgcgcggc gctgtggatc gtcgacgccg gcagcaatag agagacaaaa ctgtcgttcg
     2881 aagcgctgtc gcgccgttcc aatcaggtcg cgaatttttt gcgggcgcag gggctgaaac
     2941 gcggcgatca tctattgctg ctgctcggca acgtcattcc tttgtgggag accatgctgg
     3001 cggcgatgaa gctcggggtg gtggtgatcc ccgcgaccac gctgctgacg ccggacgagc
     3061 tgcgcgaccg gctcgaccgc ggccgcgcca gggtcgtggt ggcatcgcag gatcaggtcg
     3121 cgaaatttgc cggcctcggc ggcgaccacc tcgttcgcat cgtcgtcagc gcctcatcaa
     3181 agcatgaggg ctggctggct tacgaacagg ccgcggattt tccgcaaacc ttcgagccgg
     3241 acgggcccac ccatgccgac gatccgatgc ttttgtattt cacctccggc accacggcaa
     3301 agccaaagct ggtgcggcac agccagcgca gttatcccgt cggcgggctg tcgacgatgt
     3361 actggctcgg gctgcagccg ggcgatgtgc atctgaacat ctcctcgccg ggctgggcca
     3421 agcacgcctg gagctgcttt tttgcgccct ggaatgccgg cgccacggtg ttcgtggtca
     3481 atcagccgcg cttcgacgcc aagggacttt tggcgaccat cgggcgctgc ggcgtcacca
     3541 cgctctgcgc gccgccgacg gtgtggcggc tgttcatcca ggagaagctt gcgaccttca
     3601 aggtatcgct gcgcgaggtc tgcggtgccg gcgagccgct caatccggaa gtgatcgatc
     3661 aggtgcgcgc ggcctggggg ctgacgatcc gcgacggcta cggccagacc gaaaccacgg
     3721 cactggcggg gaattcgccg ggacagaagg tgaaggtcgg atcgatgggc cggccgttgc
     3781 cgggttatca ggtgcagatc accgatatcg acggacaggt gacgaacgaa ggcgaggtca
     3841 gcctggtgct cggagctgac tggccggcgg ggctgatgca gggttaccag cgtgacgacg
     3901 gcacattgag cggcgccgac ggcgatctct atcgcagcgg cgacgtggtg ttcgccgacg
     3961 acgagggcta tttgaccttc gtcggccgct ccgatgacgt cttcaaatca tccgattacc
     4021 gcatcagccc gttcgaactg gagagcattt tgctcgagca tgaagcggtc gccgaggccg
     4081 cggtggttcc cagtcccgat cccattcgtc tggcaatccc caaggcctat gtgctgctgg
     4141 tgtcgggcgt ggcgcgttcg ccggcgaccg cgctgtcgat ctttgcgcac ctgcatacgc
     4201 ggctggcgcc gttcaagcgc atccggcgga tcgaactggt gacggaattg ccgaagacga
     4261 tttccggcaa aatccgccgg gtgcagttgc gccggctgga gcacgacaat gatcgcgacg
     4321 acccgctgcg cggccaggaa ttccgcgaag aggagtttcc ggaattgcag acgttgcggt
     4381 cggccggatc gccggagaat tgagcaatga acgaagtctg gaagaagccg ccggtttcgc
     4441 ttgaagccta ccagaacatg gtcggccagg aggtcggagt gtcgtcatgg catctggtcg
     4501 accaaaaccg gatcgacctc tatgccgatg tcatcgagga tcatcagttc atccatgtcg
     4561 atcccgagcg cgccaaacgc gaaacctctt atggcacgac ggtggcgcac ggattcctga
     4621 ccatgtcgct gatgagcatc atgtcgta
//
LOCUS       DASWGM010000647         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2578422,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000647 DASWGM010000000
VERSION     DASWGM010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578422"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VGA15_18200"
     CDS             complement(<1..437)
                     /locus_tag="VGA15_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589662.1"
                     /translation="MSEAPVMTAWGRMARLAGSGTIATLVLLLLALWAVFAIAIGDRF
                     FSVNTLQSMAFQMPELGILSLAMMLALLSGGLNLSIIATANLAGLTIAFVLTRTIPGS
                     EGITWVGVQVLAIIAGFAVAALVGLVNGFVIAYLGVSPILATLG"
     gene            complement(434..1438)
                     /locus_tag="VGA15_18205"
     CDS             complement(434..1438)
                     /locus_tag="VGA15_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589663.1"
                     /translation="MPESLVASFGRFRRSHEFWLLIVILVLCASLGLASAQFLTVQNL
                     FDLLTSYAFVGILALGLLVVLIAGGIDISFTASASVAQYVALAAANAYGANWASVFAI
                     AIGIGGALGAVNAILIQKLRIPSIIVSVATLNVFYGLLIFFTGGKYIYSLPDWFATGI
                     FWLEFEWGKDTSYGVNLQILALAVCFLVTWLLLNRSNIGRQIYAMGGNADAARRLGFH
                     IFGLNMLVYCYMGVMAGVASLVQAQLAQSVAPTVLVGKELDVVAAVVLGGASLMGGVG
                     TVLGTFLGLALLAILQNGLILLGVSSYWSPFFVGLVILISVSATAWSQRERRTRRARL
                     "
     gene            complement(1431..>2639)
                     /locus_tag="VGA15_18210"
     CDS             complement(1431..>2639)
                     /locus_tag="VGA15_18210"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX9589664.1"
                     /translation="PNLNVAENIAMAHHLGGLRRVDWPALRATAMAAMAKVGVELDPE
                     TKVSDLSIAGRQLVAICRALAADAKLLIMDEPTASLTRHEVDALIKLVSELKRTGICI
                     VFVSHRLDEVLEIAERVTVLRDGRKVGTFAAETIDDRKLSHLMTGKAFEYQVQARGVA
                     TGPVVLEVEGLGRDGDYHDVSFQLRAGEIVGLTGLLGSGRTELALSLFGMNPPDRGTI
                     ALQGRQIALNTNADAMDLGIAYVSEDRLTLGLILDQSITANITLTMLERLAGAFGLIG
                     ARARQIHVARWVAELAIKVSNPENPVKTLSGGNQQRVVLAKWMATNPRVLILDGPTVG
                     VDISAKDGIYEIIQRLAREGVAVLMISDEIHEVLYHSHRVLVMREGRVTTDVPAEHTS
                     EDALRQAVNA"
BASE COUNT          548 a          826 c          825 g          440 t
ORIGIN      
        1 ccgagtgtgg ccaggatcgg tgaaacgccg agataggcga tgacgaatcc gttgacgagg
       61 ccgaccaagg ctgcgacggc aaaccccgct atgatcgcca gcacctgcac gccgacccag
      121 gtgatgcctt cgctgccggg aatagtgcgc gtcagcacga aggcgatggt cagtccggcc
      181 agattggcgg tggcgatgat cgaaaggttg agcccgccgg acagcagcgc cagcatcatc
      241 gcaagcgaga ggatgccgag ttccggcatc tggaaggcca tggattgcag cgtgttgacg
      301 gaaaagaacc gatcgccgat cgcaatcgcg aacaccgccc agagtgcaag cagcagcagg
      361 acgagcgtgg caatcgttcc gctgccggcc agacgggcca tgcgccccca agcggtcatg
      421 acgggcgcct cgctcatagg cgcgccctcc gggttcgtcg ctcgcgctgc gaccaggccg
      481 tcgcagaaac cgagatgaga atgaccagcc cgacaaaaaa cggcgaccag taggacgaaa
      541 cgcccagcag aattagccca ttttgcaata tggcgagcaa tgcaaggccc agaaacgtcc
      601 cgagaacggt gccgacgcca cccatcaggc tggcgccgcc gagcacaacg gccgcaacca
      661 cgtcaagctc cttgcccacc agcaccgtcg gcgcgacgga ctgcgcgagt tgcgcctgaa
      721 ccagcgaggc gacgcccgcc ataacgccca tgtagcagta gaccagcatg ttgaggccga
      781 aaatgtggaa accgagccgc cgcgccgcat cggcgttgcc gcccatggcg tagatctgcc
      841 ggccgatatt gctgcggttg agcagcagcc aggtgacgag aaagcagacc gcgagcgcga
      901 gtatctgcag gttgacgccg taggaggtat cctttcccca ttcgaactcg agccagaata
      961 tcccggtcgc gaaccagtcg ggcagcgaat agatgtattt gccgccggtg aagaaaatca
     1021 gcagcccata gaagacgttc agcgttgcga ccgagacgat gatcgatgga attcgaagtt
     1081 tctggatcag aatcgcgttg acggcgccaa gcgcaccgcc aatcccgatc gcgatggcga
     1141 agacgctggc ccagttcgcg ccataggcgt tcgccgccgc aagcgccaca tattgcgcca
     1201 ccgatgccga tgccgtgaag gagatgtcta tgccgccggc gatcaacacc acgagcagcc
     1261 caagcgcgag aatgcccacg aacgcatagg aggtcagcag gtcaaaaagg ttctgcaccg
     1321 tcaggaactg ggcgctcgcg aggcccaggc tggcgcaaag cacaaggatg acgatcagca
     1381 gccagaactc gtggctgcgc cgaaacctgc cgaatgaagc aacgagcgat tcaggcattg
     1441 actgcctgtc gaagcgcgtc ttcggacgtg tgttcggccg gcacatcggt tgtcacacgg
     1501 ccctcgcgca tcaccaacac gcgatggcta tgataaagca cctcgtggat ttcgtcggaa
     1561 atcatcagca ccgcgacgcc ctcgcgggcc agccgctgga tgatctcgta gatcccgtcc
     1621 tttgcgctga tgtcgacgcc gaccgtcggg ccgtcgagga tcagcacgcg cgggtttgtc
     1681 gccatccatt tggcgagcac gacccgctgc tggttgccgc cggagagtgt cttcaccggg
     1741 ttttcggggt tcgagacctt gatcgcgagt tcggcgaccc atcgcgcaac gtggatttgc
     1801 cgcgcgcgcg cgccgatcag accgaatgcg ccggcgaggc gttccagcat cgtcagcgtg
     1861 atgttcgccg tgatcgactg atccaggatc agaccaagcg tcaacctgtc ctcggaaaca
     1921 taggcgatgc cgaggtccat cgcgtccgca ttggtgttca gcgcgatctg gcggccttga
     1981 agcgcgatcg tgcctcgatc gggtgggttc atgccgaaca gcgacagcgc gagctcggtt
     2041 cggcccgaac cgagtaatcc ggtgaggccc acgatctctc cggcgcgcaa ctgaaagctc
     2101 acatcgtgat agtcgccatc tcggcctagg ccttcgactt cgagcacgac cgggcctgtg
     2161 gcaacaccgc gcgcctgcac ttgatattca aaggcctttc ccgtcatcag atggctgagt
     2221 ttgcggtcgt cgatcgtctc cgcggcgaag gtgccaactt tcctgccgtc ccgcagcacg
     2281 gtcacgcgct cggcgatctc gagcacttcg tcgagccggt ggctcacgaa tacgatgcag
     2341 attccggtgc gtttcagctc gctgacgagc ttgatcaggg catcgacctc atggcgtgtc
     2401 agcgacgcgg ttggctcatc catgatgaga agcttggcgt ctgccgccag cgcacggcag
     2461 atcgcgacca gctggcgtcc ggcaatgctg agatcggaga ctttggtttc cggatcgagt
     2521 tcgacgccga ccttcgccat cgcggccatg gcggtcgcgc gcagcgcggg ccagtcaact
     2581 cggcgcaagc cgccgagatg atgcgccatc gcgatgttct cggcgacatt gaggttggg
//
LOCUS       DASWGM010000648         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_83273,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000648 DASWGM010000000
VERSION     DASWGM010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83273"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..919
                     /gene="dapA"
                     /locus_tag="VGA15_18215"
     CDS             8..919
                     /gene="dapA"
                     /locus_tag="VGA15_18215"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX9589665.1"
                     /translation="MHGLWLPLVTPFRNGELDEASLRRLARHYADLPVNGLILAATTG
                     ESLTLAREETERLVFAVRDEIGGAGHPSICLGLSGSNTRALLDTLDRTAAWPIDGYLI
                     SCPYYSRPSQQGLCLHFTALADRAVHPVMLYNIPYRTGVNLGNEAMLRLADHRNIAGL
                     KDCCGDRNQSLDLLHRRPENFAVLTGEDAQYHEALVDGADGGILASAHIETETFARVR
                     TLLVAGECDAALSCWRSVAVLTRLLFAEPSPAPIKYWLWRIGLIDSAELRLPMTEVSA
                     ELAGRIDLEIEGRTAVPIADQARDRPA"
     gene            1037..1189
                     /locus_tag="VGA15_18220"
     CDS             1037..1189
                     /locus_tag="VGA15_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589666.1"
                     /translation="MTIQAIQIDRSPTSKHAQTMQDIFLVSSFGLWAVLLGLAPVLAF
                     RLLSGS"
     gene            1327..1461
                     /locus_tag="VGA15_18225"
     CDS             1327..1461
                     /locus_tag="VGA15_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589667.1"
                     /translation="MSTQDNQYPGQQGFSTGDIVWALGIWSVILSLSPVVVFYVLMVN
                     "
     gene            complement(1686..2366)
                     /locus_tag="VGA15_18230"
     CDS             complement(1686..2366)
                     /locus_tag="VGA15_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9589668.1"
                     /translation="MNVIVFASRKGGSGKSTLAAHIAAHVHKATKPCLLIDADPQGSL
                     TLWHKLRGTNEPAIKTAVNSVSGIVAAAKRDGIEWVFIDTPPNMSAVVEDAIRNATMV
                     IIPARPGVFDVNAVQETILTCRAARKPYAVVINGAPACRDDAESPIVTIAREALIKFR
                     APVWGGQITNRADLLMALSHGEGAREYYAEGRAAAEIARLWSAIERSVKAIRGSVSAT
                     GTMHKQAA"
BASE COUNT          516 a          776 c          760 g          533 t
ORIGIN      
        1 actttgcttg catggcctct ggctgccgct cgtcacgccg tttcgcaatg gcgaactcga
       61 cgaggcgtca ctgcgccggc tggcgcggca ttacgccgat cttcccgtca atggattgat
      121 tctcgccgcc acgaccggcg aaagcctcac cctcgcgcgt gaggaaaccg aacggcttgt
      181 attcgcggtc cgcgacgaaa tcggcggggc cggacatcct tcgatctgcc ttggcctgtc
      241 cggcagcaac acccgcgcat tgctggacac gctggaccga accgcggcat ggccgatcga
      301 cggctacctg atttcctgcc catactattc gcggccgtcg cagcagggcc tctgccttca
      361 tttcacagca cttgccgatc gcgctgtgca tccggtcatg ctctacaaca tcccctaccg
      421 gaccggcgtc aatcttggca acgaggcgat gttgcggctg gccgaccatc gcaacatcgc
      481 cggcctcaag gattgctgcg gtgaccgcaa ccagtcgctc gatctgttgc accggcgccc
      541 cgaaaacttc gccgttctca ccggcgagga tgctcaatat catgaggcgc tggtcgatgg
      601 cgccgacggc ggcatcctgg catccgcgca tatcgaaacc gaaaccttcg cacgggttcg
      661 gaccctgttg gtggcaggcg agtgcgatgc cgcattatcg tgctggcggt cggttgcggt
      721 gctcacccgg ctgttgttcg ccgaaccgag cccggcaccg atcaagtact ggctgtggcg
      781 tatcggattg atcgacagcg cggaactgcg gttgccgatg acggaagtga gcgccgagtt
      841 ggccggccgg atcgatctgg aaatcgaagg gcgaacggcg gtgccgatcg cagaccaagc
      901 ccgcgatcgg ccggcgtaat caagccgtct ggttaatgcc cggtaatttc ggcatttcgc
      961 atccgaacga ataggcggct cggtccttaa aattcaaggc ttatccaaat tccacaacaa
     1021 aacattgggg gaagcgatga ccattcaagc cattcaaatc gacaggtctc cgacgtccaa
     1081 acacgcccag acaatgcagg atatctttct ggtgtcgtct ttcggtcttt gggcggttct
     1141 gctgggcctg gcgccggttc tggcgttccg cctgctgagc ggaagctgac cagagccggc
     1201 gggtgcccgg ctggttaatc cgcggtagca cctgcgttca aatgcgagcg atctcgcaac
     1261 cggcttttta gaactcgacg cgtatgaaag gtctgtcgga aagaacaaga accgcggggg
     1321 cgcgtcgtga gtactcagga caatcaatac cctggacaac agggtttttc taccggggac
     1381 attgtttggg cgctcggcat ctggtcggtg attctgagcc tgtcgccggt cgttgtgttc
     1441 tacgtgctga tggtgaacta gaagcgtttt caagcgaagt ggaaaccggt tcgcgtcaag
     1501 aaaacgcgtc aaataaaaga atctagaggc ccgtttcgat tctatcgaaa cgaggctcta
     1561 gttcacccct aaacaacgtc atccccgcgt gcgcgcaatg acgcccacgg gggatgacgt
     1621 ttggtcacga caataagcgc gcgcgggact ttgcgcgttc tttatcggca ggccgcgcga
     1681 cgaagctagg ccgcctgctt gtgcatcgtg ccggtcgccg acaccgatcc gcggatcgcc
     1741 ttcaccgagc gttcgatcgc agaccatagc cgagcaatct ccgccgccgc acggccttcg
     1801 gcataatatt cgcgcgcgcc ttcgccgtga ctgagcgcca tcagcagatc ggcgcggttg
     1861 gtgatctggc caccccacac cggcgcgcgg aacttgatca gcgcttcacg cgcgatggtg
     1921 acgatggggc tctccgcgtc gtcccggcag gccggtgcgc cgttgatcac gacggcatag
     1981 ggcttgcgcg ccgcgcggca ggtcaaaatc gtttcctgca cggcgttgac gtcgaatacg
     2041 cccggccgcg ccggaataat caccatcgtg gcattcctga tggcatcttc cacgacggct
     2101 gacatgttcg gcggcgtatc gatgaatacc cattcgatcc cgtcgcgctt ggcggcggcg
     2161 acgatcccgc tgacggaatt gacggcggtt ttgatggccg gttcgttggt accacgcagc
     2221 ttgtgccaga gtgtcaatga accctgcgga tctgcatcaa taagcagaca gggcttcgta
     2281 gccttatgga catgcgcagc aatatgagct gccagggtgc tcttccccga gccgccttta
     2341 cgcgatgcaa aaacaatgac gttcatacct cagcctccag attgaccctt gagacaaaat
     2401 gaatcagcac gctgattcgc gaaagaaaaa tgttgtgcca attggggcga actcctggat
     2461 tattcagggc gttgactttt gagtcacacc atgtgtcgca acaggcacaa acggcgtcgc
     2521 agcaaccaag aaaatcgaac caaccagccg tttgatggag gcattgagcg attcttttgg
     2581 tccgt
//
LOCUS       DASWGM010000649         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2684589,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000649 DASWGM010000000
VERSION     DASWGM010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684589"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VGA15_18235"
     CDS             <1..141
                     /locus_tag="VGA15_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX9589669.1"
                     /translation="DGLRVMDASIMPRIISANTNATVMAVADKGVDLLTGTYTGRVAA
                     AQ"
     gene            complement(557..1459)
                     /locus_tag="VGA15_18240"
     CDS             complement(557..1459)
                     /locus_tag="VGA15_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9589670.1"
                     /translation="MDRFDAMRVFARIVERRSFTQAADDLGLPRSSVTDAVKGLESRL
                     GVRLLQRTTRQVSPTLDGEAYYQRCVSLIADMEDAEGAFVGAKPSGLVRVDVHGTQAR
                     YFLLPGLPRFRQQYPDIRLHFSEAHQPLDMIREGFDCILRAGELSDSPLIKRRLALHE
                     RGTFASPDYLGRFGTPQTPDDLLGHEMVGMLSPDSGDLVPLTFGAGGNARQVKLPAMV
                     TVTGPETNVACACMGLGLIQVPRYRVSSELASGALVEVLAGFPPSPLPVHVLYSHTRQ
                     LSPRLRVFIDWMAEQFRDRLSPTE"
     gene            1577..>1936
                     /locus_tag="VGA15_18245"
     CDS             1577..>1936
                     /locus_tag="VGA15_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9589671.1"
                     /translation="MTTPTNRVAIVTGASGGIGAAVAERLAKDGFTVVVNYAGNSASA
                     AAVVARIEAAGGRAVAAQADISDVAAVARMFDSAETAFGGVDVLINNAGIMNLATIAD
                     SSDALFDRHIAVNLKGTF"
BASE COUNT          379 a          599 c          616 g          342 t
ORIGIN      
        1 gacggccttc gggtcatgga cgcctccatt atgcccagga tcatcagcgc caacacgaac
       61 gccaccgtca tggcggtcgc cgacaagggc gtcgatcttc tgactgggac ctacacaggg
      121 cgcgttgccg ccgcgcagta aacctgcccg gccataggga ccacgccttg tccacgacgc
      181 gaagcccgtc gccatccttc ggaatggcgt gtacaggacc tgagccacca ggctgcggcg
      241 gcaagcccct gaaggttgcg tcggcttcca ggagtgtgcg acagccgggg cgcagcgaac
      301 tcgtgggaga gtggcatcgt gagcggttat ggaacggccg ctgcaaagca gccattcatt
      361 agagcgtcgc gatcaggcga aaccaaatta tatcgctatt tcaatacata tgagttggca
      421 ggtttattct gaatggcgcg cgatcggcgc tgcggatcta acggatgcgc acgagtcaat
      481 tgctgccaaa gaccaacgta tctgttcttc gccgttagtc gtgccaggcg accaacggct
      541 tggtctcatg ctcatgtcat tcagtcggtg agagccggtc tcgaaactgc tcagccatcc
      601 agtctatgaa cacccgcaag cgcggtgata gctggcgcgt atgcgaatag aggacgtgga
      661 caggcaacgg cgatggcgga aagccagcaa gcacctcgac caacgcgccg ctggcgagct
      721 ccgagctgac gcgataccgc ggcacttgaa tcaacccgag acccatgcac gcgcaggcga
      781 cattggtttc gggaccggtc accgtcacca tcgccggcaa tttcacctgg cgagcgttgc
      841 cgccggcgcc gaaggtcagc ggcacgaggt cgccgctgtc gggcgatagc attccgacca
      901 tctcgtgacc taggagatcg tcgggcgtct ggggcgtgcc gaagcggccg agatagtcgg
      961 ggctcgcgaa cgtcccccgc tcgtgcagcg ccagtctgcg cttgatcagc ggactgtcgc
     1021 tcagttcacc ggcccttagg atgcagtcga agccttcgcg gatcatgtcg agcggctggt
     1081 gcgcttcgct gaagtgcagc cggatgtcgg gatattgctg ccggaagcgc ggcaggcccg
     1141 gaagcaggaa atagcgcgcc tgggtgccgt gaacgtcaac acgaaccagc cccgacggct
     1201 tggcgccgac gaaggcgccc tccgcatcct ccatgtccgc gatcaggctc acgcagcgct
     1261 ggtaataggc ctcgccatcc agggtcggac tgacctgccg tgtcgtgcgc tgcaatagcc
     1321 gtacccccaa ccgcgactcc agtcccttca cggcatcggt caccgaggat cgcggcaggc
     1381 caagatcgtc ggcggcctgg gtgaaactgc gccgttccac gatgcgggcg aagacgcgca
     1441 tggcgtcgaa gcggtccatt gtacgatctt tcgaataatg atgtcgaaag atgcacgatt
     1501 atccgttcca tgggaatggg catgttgtct ccgtccagtg ccgatcatcg gcgcccaacc
     1561 cataaggaga aagatcatga ccaccccaac caatcgagtt gccatcgtca cgggtgcttc
     1621 gggcggcatc ggcgccgcgg ttgccgagcg cctggcgaag gacggcttca ccgtggtggt
     1681 gaattatgcc ggcaacagtg cttcagcagc ggcggtggtc gccaggatcg aggcggcggg
     1741 cggccgggcc gtggcggccc aggccgacat ctcggatgtc gccgccgtgg cacgcatgtt
     1801 cgactcggct gaaaccgcgt tcggcggcgt cgacgtgctg atcaacaatg cgggcatcat
     1861 gaatctggcg accatcgctg acagcagtga cgcactgttc gatcgccaca tcgcggtcaa
     1921 tctgaagggc acgttc
//
LOCUS       DASWGM010000650         5038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1749642,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000650 DASWGM010000000
VERSION     DASWGM010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749642"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGA15_18250"
     CDS             complement(<1..213)
                     /locus_tag="VGA15_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589672.1"
                     /translation="MRKRALRIRPFAWTAVIAALACLFGAAAQAQQPICSAWLIESSR
                     FMNVPFGAFMPDRAFVPGSTKPESNIP"
     gene            complement(321..875)
                     /locus_tag="VGA15_18255"
     CDS             complement(321..875)
                     /locus_tag="VGA15_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589673.1"
                     /translation="MTGGRSLLLAGVVVALAATAGAAWLHEARPRSAAQPAAQTVSTR
                     AEKPQPPALTADEERFATALWAVHREATRLAVAMSFAGIAYQTEDRDARALARKIEPL
                     AKFFHDAEMQVRTISTPPSLSTAHGQYVEAMALYANAAAEMLKFTEDGDLQHLGDAHR
                     MDVRASEDMLRVGEVLWPGQYKPH"
     gene            complement(1147..1983)
                     /locus_tag="VGA15_18260"
     CDS             complement(1147..1983)
                     /locus_tag="VGA15_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589674.1"
                     /translation="MTGTALNVVSFLFLGFFLGMRHATDADHVVAIATIVSRERSVAG
                     SALIGAAWGVGHSITVMAVGGAIILFGVVIPPQLGLSMEFAVGIMLVLLGVLTLTGMG
                     RAVGAAHAHAGVPGGHALDLHEHLHAHGDYVHQHPHGHGPGAHGHAEEHTPLARLDRS
                     GLGRIAFYEWLRPFAVGLVHGLAGSAAVALMVLSIIREPVAALGYLLLFGLGTIVGMM
                     LITLILSAPFVFTAVNLPKFNWRLRVASGLVSFVFGVVLIYSIGFAEGGLFTDAPSWE
                     PR"
     gene            complement(2296..3027)
                     /locus_tag="VGA15_18265"
     CDS             complement(2296..3027)
                     /locus_tag="VGA15_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589675.1"
                     /translation="MPPTEIQTFFTGVDASPDFTPAVVIVALATASHRILSGSTLLDC
                     RLQSGEELEQHRPSCGLARNASGVHRGRHRTLNFVVVKLTRGVPICHPRALHGMRFGR
                     LAIIERIQQALVIFVEQSVPDRIFYAVAVGLSRFVGITRWLVDAGVRDKIEQALPFAL
                     IQIIPVLGRAIPDRRDFMRGGFSGLRVASFSGKAQRQLQSARQSGAERRIESRSHSSF
                     FLAAPLIPRVLSVSIEPKRGSRFLI"
     gene            3179..4432
                     /locus_tag="VGA15_18270"
     CDS             3179..4432
                     /locus_tag="VGA15_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589676.1"
                     /translation="MVTSQRSWPVDRRTILKTSAALAAVQFSSPFIIKARGETPVRIG
                     MVDPLTGVYAAIAQGEVVGAKFAVEEINKKGGILGRPVELLIEDSANDVGTGVQKTRK
                     LIERDQVSFIVGDVNSGIAIAMAQVTSEKKVLHIVSGGHTDPITGANCSWNVFRVCNS
                     TTMDANAIATTLIEKFGKKWYFLTPDYAYGHSVQAGFEKVLKAAGGTSAGSLVPLGTP
                     DYSAYLIQAKAYGPQVLINVMGGGDQVSSLKQFVQFGLDKQMAVGGALFELESIRAVP
                     DGARVGWWTMEWWWDQPGVPHVAEFNASIKKITGGAATARNWFGYVSIQTLAVLANQE
                     KTLEAPRLARALSGFKLPPEIALQPGTPEYRAGDHELMSTVFVGEAHPPQRDPDNMFT
                     IRNPVPGEKAAGPVEGTGCKIQWPA"
     gene            4524..>5038
                     /locus_tag="VGA15_18275"
     CDS             4524..>5038
                     /locus_tag="VGA15_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589677.1"
                     /translation="MTSQISNPKFDRRTLIKGAAALGAFQVASPFIIQARGETAIRIG
                     MVDPLTGVYAAPAGNEVMGAKLAVEQVNAKGGILGRQVELLVEDSANDVGTGVQKARK
                     LIERDQVSFMIGDVNSGIAAAIAQVTSEKKILHIVSGGHTDSITGKDCKWNVFRVCNT
                     TRMEANSVSSVL"
BASE COUNT         1030 a         1570 c         1537 g          901 t
ORIGIN      
        1 cggaatgttg ctctccggct tggtgctgcc gggcacgaat gcgcgatccg gcatgaacgc
       61 tccgaacggc acgttcatga aacggctgct ctcgatcagc catgccgagc agatcggttg
      121 ctgggcctgc gccgccgcgc caaacaggca tgccagcgcc gctatcacgg cggtccaggc
      181 gaacggacga attcgtaacg cgcgttttct cattgccttc ctccctacag cttgattgag
      241 cttctagatt cagctttctt gattgagatt ggactgcgct tggctcttct tctgcgattc
      301 tcctgcgatc cgattgcccc ctagtgcggc ttgtattgtc ccggccacag cacctcgccg
      361 acgcgcagca tatcttccga agccctcaca tccatccggt gggcgtcgcc gagatgctgc
      421 aggtcgccat cctcggtgaa tttcagcatc tcggcggcag cgttggcgta gagcgccatt
      481 gcctcgacgt actgcccgtg cgccgtggac agcgagggcg gcgtgctgat ggtgcggacc
      541 tgcatttcgg catcatgaaa gaatttcgcc agcggctcga tctttcgtgc gagcgcgcgc
      601 gcgtcccgat cctcggtctg ataggcgatg ccggcaaagc tcatcgccac cgccaaccgg
      661 gtcgcctccc ggtggacggc ccacaaggcc gtcgcgaagc gttcctcgtc cgccgtcaat
      721 gccggcggct ggggtttctc ggctcgcgtg ctgacagttt gcgccgccgg ttgcgccgcc
      781 gaccgcggcc gcgcctcgtg cagccacgcg gcacccgcgg ttgctgcgag cgccacaacg
      841 acgccggcca gcaacaacga ccgcccaccg gtcatgccga tcgtctgaat ggcgtatcct
      901 ccatgtattg ttgcggcact gtatccctcg atttccgcgg cggtcaaccg gcgcgacggc
      961 gaaccagacc ctgcccgcac tcatggcttg cggttgaccg cgagccatgc gctagagcgg
     1021 gatgactttt cttcgaatcg tcatcccgct ctatcttttt gtttgagcat gatcttttcg
     1081 gaaaaccggt aaccacccat ccggtcgtgg cccgagggca tgcttttccg gatcatgctc
     1141 cagcggtcaa cggggttccc aactcggcgc gtcggtgaac aggccgccct cggcaaagcc
     1201 gatgctgtag atcaggacca ccccgaagac gaagctgacc aggcccgacg ccacgcggag
     1261 ccgccaattg aatttcggca gattgaccgc cgtgaacaca aacggcgccg acaggatcag
     1321 cgtgatcagc atcattccga cgatagtgcc gagcccgaac agcagcagat agccgagcgc
     1381 ggcgaccggt tcgcggatga tggacaggac catcagggcg acggcggccg atcctgcgag
     1441 gccgtgcacg aggcctaccg cgaacggtcg cagccactca tagaacgcga tccggccgag
     1501 cccactgcgg tcgagccgcg ccagcggcgt gtgctcctcg gcgtgcccgt gcgcaccggg
     1561 cccgtgaccg tgcgggtgct ggtgcacgta gtcgccatgc gcatgcaggt gctcgtggag
     1621 gtcaagcgca tgtccgccgg ggacgcccgc atgggcgtgg gccgcgccga ccgcgcggcc
     1681 catccctgtc aaggtgagaa cgccgagcag gaccagcatg atgccgacgg cgaattccat
     1741 cgacagaccg agttgtggcg ggatgacgac cccgaacagg atgatcgccc cgccgaccgc
     1801 catgaccgtg atggagtgcc cgacgcccca ggcggcgccg atcagcgccg agcccgccac
     1861 gctgcgctcg cgactgacga tcgtcgcaat ggcgaccaca tggtcggcgt cggtcgcatg
     1921 ccgcatcccg aggaagaagc cgagaaacag gaaggacacg acgttcaggg cggttccggt
     1981 cattgcgcat gcgccaaggt ggttatcccc ggccgtatta tgcagcgttt cgacccaacg
     2041 ccaattccca tatgcgtgcg ccccgtggca cggctgcggt gcggggccga tcggcagctt
     2101 cccgccgccc cctcatgcgc tgcccccgcc gatgccctct tcaccaacgg aatcacggat
     2161 tgcgagtcgc ctcaacagga gttttgcaac ataatcgtgg gtaaagcgga catcgcgatc
     2221 cccaacgcgt tttcgagcga aagcctgccc cggacttgat ccggggtgga aaccggttcg
     2281 cgtcaagaaa acgcgtcaaa tcaagaatct agagccccgt ttcggctcta tcgaaacgga
     2341 aagtactcta gggattaaag gcgcagcgag gaagaacgac gaatgagatc tcgattcaat
     2401 ccggcgttca gcgccgcttt gacgtgcgct ttgcaactgg cgctgagctt tgccgctgaa
     2461 cgaagctacg cgcaaacccg aaaacccacc gcgcatgaaa tcgcggcgat ccgggattgc
     2521 gcgaccaaga acagggataa tctggatgag ggcgaacggc agtgcctgtt caatcttgtc
     2581 gcggacccct gcatcaacaa gccatcgggt gatgccaaca aaccgactga ggccgactgc
     2641 taccgcatag aagattcgat ctgggacgga ttgctcaacg aaaattacaa gagcctgctg
     2701 gatacgctcg atgatggcca gacggccaaa gcgcatgcca tgcagcgcgc gtggatggca
     2761 tatcgggaca ccacgtgtca gttttacgac gacaaaattc aaggttcgat ggcgaccgcg
     2821 atgcacgccg cttgcgttac gcgcgagtcc gcacgacggg cgatgctgct caagttcttc
     2881 accggattgt aagcggcaat caagcaacgt cgatccagac aggatgcgat ggctggcggt
     2941 agccagggcc acaatgacga cggccggcgt aaaatcgggt gatgcgtcaa cgccggtgaa
     3001 gaatgtctgt atttcggtgg gcggcatatt ccatgcgcac gcgtcataga ccaaaggtcg
     3061 caggattttc agaggcttgc tattggcaaa agcaatcggg aagggctacg ctcccggcct
     3121 atcaaggcat cgaagaatgc ctgccatcaa gatgcgtcac taggtctcgg aggacagcat
     3181 ggttacttcg caacgatcct ggccggtcga tcggcggact attctaaaga ccagcgccgc
     3241 gcttgctgcg gtgcaatttt catccccctt catcatcaag gcccgtggcg aaacgccggt
     3301 gcggatcgga atggtcgatc cgctgacagg cgtttatgcc gcgatcgccc agggcgaggt
     3361 ggtgggggca aaattcgccg tcgaggagat caacaagaag ggcggcattc tgggccggcc
     3421 ggtcgagctc ttgattgaag attcggccaa cgatgtcggc accggcgtgc agaagacacg
     3481 gaagctgatc gagcgcgatc aggtgagctt cattgtcggt gacgtgaatt ccggtattgc
     3541 catcgcgatg gcacaggtca ccagcgaaaa gaaggtgctc cacatcgtct caggcggaca
     3601 caccgatccc atcaccggcg ccaactgttc gtggaatgtg ttccgggtct gcaattccac
     3661 caccatggac gccaatgcca tcgccaccac gctgatcgaa aagttcggca agaagtggta
     3721 cttcctgacc ccggactacg cctatggcca ctcggtccag gccggctttg aaaaggtcct
     3781 gaaggccgcg ggcggcacct ccgccggctc cctcgtcccg ttgggcacgc cggattattc
     3841 ggcttacctg attcaggcca aggcctacgg cccgcaagtg ctcatcaatg tcatgggcgg
     3901 cggcgatcag gtctcgagcc tgaaacaatt tgttcaattc ggcctcgaca aacaaatggc
     3961 cgtcggcggc gccctgttcg aactggaaag cattcgcgcc gtgcccgacg gcgcgcgggt
     4021 cggatggtgg accatggaat ggtggtggga tcagccgggc gtgccgcatg tcgcggaatt
     4081 caatgcctcg atcaagaaaa ttaccggtgg cgcggccacg gcgcggaact ggttcggcta
     4141 tgtgtcgatc cagacattgg cggtgcttgc caatcaggag aagacgctcg aggccccaag
     4201 actggcgcgc gcgctttcgg gattcaagct gccaccggag atcgccctgc aacctggtac
     4261 gccggagtat cgcgccggcg accacgaatt gatgagcacc gtgtttgtcg gcgaagcgca
     4321 tccgccccag cgcgatccgg acaatatgtt cacgatacgc aaccccgtgc ccggcgaaaa
     4381 agccgccgga ccggtagagg gcaccggctg caagattcag tggccggcct gacacaggac
     4441 gatacgtcgc ctggtgactc aaacggcacc gggcggggta aaacttctca agaagaaacg
     4501 caacaaaccc agagagggaa cccatgacat cgcaaatttc caatccgaaa ttcgaccgtc
     4561 gcacgctgat caagggtgcc gccgcgctgg gcgcctttca ggtggcatcg cccttcatca
     4621 tccaggcgcg cggcgagacc gcgatccgga tcggcatggt cgatccctta accggcgtct
     4681 acgcggcccc tgccggcaac gaggtgatgg gagccaagct cgccgtcgag caggtcaacg
     4741 ccaaaggcgg cattctcgga cgccaggtcg aattgctggt ggaagattcc gccaatgacg
     4801 tcggcaccgg cgtgcaaaaa gcccgcaagc tgatcgagcg cgatcaggtc agcttcatga
     4861 tcggcgacgt caattccggc attgccgccg cgatcgcgca agtcaccagc gaaaagaaaa
     4921 tcctgcacat cgtttccggc ggccataccg attcgattac cggcaaggat tgcaagtgga
     4981 atgtgttccg cgtctgcaac accacgcgga tggaagccaa ttcggtatcc agcgtgct
//
LOCUS       DASWGM010000651         6762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2019866,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000651 DASWGM010000000
VERSION     DASWGM010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019866"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            562..837
                     /locus_tag="VGA15_18280"
     CDS             562..837
                     /locus_tag="VGA15_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589678.1"
                     /translation="MSNVFAECDAGFPWLAGAMLAFESCEVIHLRLAKLAIGDHDAEC
                     EAQLMVNEKINAMFEAGASLMSGASAAAVIGRYREHVAANAKRLSAI"
     gene            complement(927..1697)
                     /locus_tag="VGA15_18285"
     CDS             complement(927..1697)
                     /locus_tag="VGA15_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9589679.1"
                     /translation="MKIATFNINNINRRLPNLLRWLRAAKPDIVCLQELKSTDADFPL
                     STIEKAGYGAVWRGQKTWNGVAILARNADPVLIRTELPGAPDDHEARYIEAAVSGIIV
                     TSIYLPNGNPQPGPKFDYKLDWFRRLKLHAAKFLKQDIPVVLAGDYNVAPTELDIYPT
                     RSWDKDALIQPKSRAAFKSLVAQGWCDAIRELHPSKPMFTFWDYKRNRWPRDAGLRLD
                     HLLLSPAVAPRLIKAGVDCKLRGEEGASDHAPAWIVLS"
     gene            complement(1713..2972)
                     /gene="alr"
                     /locus_tag="VGA15_18290"
     CDS             complement(1713..2972)
                     /gene="alr"
                     /locus_tag="VGA15_18290"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX9589680.1"
                     /translation="MNIVPDPKSIPTSAVLSPEANQATALATAPGVLTVDLDAIVANW
                     RKLEKTAVPAECAGVIKADAYGCGVGPVARALAGAGCKTFFVATLDEARAARAAIPSA
                     AIYVLSGFFQNSGEAFARIDARPVMGDLNELAEWDVFCRRSGWAGGAAIHIDTGMNRL
                     GLTAAEAQGIIPRINAGDHGITLVMSHLACAEILHHPLNAKQLASFREIASLFSGVPA
                     SLSNSSGVFLGGQFQFDMVRPGAALYGVNPTPEADNPMQPVVELKARIAQIRNVEKGE
                     TVGYGGTWTARRPTKLAIVSVGYADGYFRAGSSNDGTRGADVVVAGKRCPVAGRISMD
                     LMAVDITDLAHNAARRGHLVTLIGEGITVDELAHHFGTIGYEVLTSLGPRYARVYKGG
                     AAATPEQPAPVAEPAPAAVQSTSAAPA"
     gene            complement(3021..4520)
                     /locus_tag="VGA15_18295"
     CDS             complement(3021..4520)
                     /locus_tag="VGA15_18295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX9589681.1"
                     /translation="MAPFDSNVLKLAPDAGTPAYRSAPHNIEAEQSLLGAILVNNDAF
                     YRVSDFLEPKHFFEPIHQTIYETAGSLIRMGKVATPVTLKTFVPAETDIGGMTVGQYL
                     ARLAAEATTIINAQDYGRTIYELALRRDLIRIGEDMVNVAFDAPVDFAPRAQIEDAER
                     RLYELAESGRYDGGFQRFAQALTVAVDMAAKAFQRDGKLSGVATGLRDLDTKMGGLQP
                     SDLIIVAGRPGMGKTALATNIAYNVAKAHRAEVQADGTMKSVNGGIVGFFSCEMSAEQ
                     LATRILAEQTSISSSSIRRGGITEADFEKIRDYSIELQSLPLYVDETGGLSISQLTAR
                     ARRLKRQKGLDLIVVDYIQLLQGSGKRSDNRVQEVTEITTSLKALAKELNVPVIALSQ
                     LSRQVESRDDKRPQLSDLRESGSIEQDADVVMFVFREEYYLANKEPRVGTPEYEKWQL
                     DMSLVHGKAEVIIGKQRHGPTGTVELQFEGQFTRFGDLAQDSHLPAQTY"
     gene            complement(4818..4940)
                     /locus_tag="VGA15_18300"
     CDS             complement(4818..4940)
                     /locus_tag="VGA15_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589682.1"
                     /translation="MDEKTYPSPFDPQRDIGTDYALIAVGVGAALIALLYLLLI"
     gene            5081..5287
                     /locus_tag="VGA15_18305"
     CDS             5081..5287
                     /locus_tag="VGA15_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9589683.1"
                     /translation="MGQQLQPWRSENGQTRWLSMLIDAMEKVSRPEFRAVPCDPWVLA
                     AVRAQLARPASPYALGASRFSLRN"
     gene            complement(5318..6568)
                     /locus_tag="VGA15_18310"
     CDS             complement(5318..6568)
                     /locus_tag="VGA15_18310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX9589684.1"
                     /translation="MHRLLRYFLQQFIRRGAMTFTTASGATFSCGDGTGEPVCVRFLT
                     ADAERRVLLNPELALGEVYMDGTFVVEKGSIADALAILMDQPEILPRWAKLQWWLRYL
                     ARHAKQFNPPGRSKNNVAHHYDLDGRLYSLFLDADKQYSCAYFEAPDAALDDAQLAKK
                     RHLAAKLLIGHGDRVLDIGSGWGGLGLYLAEMTGANITGITLSTEQLEVSNARAAEKN
                     LAASAKFFLEDYRDISGPFDRIVSVGMFEHVGVDFYETYFRRCAELLTDDGVMLLHSI
                     GRSDGPDVTSPWITKYIFPGGYIPALSEVMPAIERAGLLVCDIEILRLHYAETLKAWR
                     ERFMARREEAVRLYDERFARMWEFYLASSEMSFRKQNLMNFQIQLSKRQGVVPMTRDY
                     IAHEEAKLRGIERGKRPRLQIAGE"
BASE COUNT         1276 a         2039 c         2119 g         1328 t
ORIGIN      
        1 atcaaaagcc acaaaagctt cgatatgatc gttcatgact cgccctcctt gcgtgaggct
       61 ctgcccggcc aatccgggta accctcgcac tcgcatcgag ggcgagtcgc ctccgcaaga
      121 aaagggacat acggtcttcg cggagcctgt catcgggcgc gcattcgcgc gacccggtgg
      181 ctcgcaatga cggtgcggct gtttgaaaat tgaatcggga attagagtgc gcggcacttc
      241 ctaacgtgcc tcacgaaacg cacccgtcat gcccggattt gatccgggca tccatcaatc
      301 ttcgtaacaa tttttttcga agaggatgga tcaccgggtc atccagcgcg aagacgcgct
      361 tcgcgctttc gcccggtgat gacgatctca attggtatga cggtcttcgc tccccgcaat
      421 gacgagaagt tggtggcgtc tcgccctcgt ggttaaccag ccgtagctcc tgtgccgaaa
      481 atgtcagcgg tctcgcgcct tagtccttaa aatttgacgc gcattaaact catctcgaat
      541 tcaaaagatc tggaaaccgc gatgagcaac gtctttgcgg aatgtgatgc cggctttccc
      601 tggctcgccg gcgccatgct ggcattcgag tcctgcgaag tcatccatct tcggctggcg
      661 aaactcgcga tcggcgacca cgacgcggaa tgcgaggccc agctgatggt gaacgagaag
      721 atcaatgcga tgttcgaagc cggcgcgagc ctgatgtccg gcgcgtcggc tgcggccgtc
      781 atcggccgct accgcgagca tgtcgccgcc aatgcaaaac ggctttcggc catttagcgg
      841 cgcgtattct accgcagata agtctacgca atctgcgtaa acttgattgc gaagcaaaac
      901 cggtacccac ttttgcggaa tacgcgctaa ctcaaaacga tccacgccgg cgcgtggtcg
      961 ctggcgcctt cttcgcctct cagcttgcag tcgacgccgg ccttgatcag ccgcggtgca
     1021 accgccggac tcagcaagag atgatcgagc cgcaagccgg cgtcgcgcgg ccagcggttg
     1081 cgcttgtaat cccagaacgt gaacatcggc ttggacggat ggagttcgcg gatcgcgtca
     1141 caccagcctt gcgcgacgag ggatttgaat gccgcgcggc tcttcggttg gatcagcgca
     1201 tccttgtccc atgaccgcgt cggatagata tccagctccg tcggtgcgac gttgtagtcg
     1261 ccggccagca cgaccgggat atcctgcttg aggaatttcg cggcgtgcag cttgagccgc
     1321 ctgaaccagt cgagcttgta gtcgaatttc ggcccgggct gcggattgcc gttcggcagg
     1381 tagatgctgg tgacgatgat gccgctgacg gcagcctcga tatagcgcgc ctcgtggtcg
     1441 tcgggagcgc caggcaactc ggtccggatc aacaccggat cggcgttgcg cgccaggatc
     1501 gcgacaccgt tccaggtctt ttggccgcgc cagaccgcgc cgtaaccggc cttctcgatc
     1561 gtagaaagcg gaaaatcggc gtcggtcgat ttgagttcct gcaagcaaac gatgtcgggt
     1621 tttgccgcgc gcagccagcg cagcaggttg ggcagccgcc ggttgatgtt gttgatgttg
     1681 aaggtggcga tcttcatgcc tgactgaaga cgtcaggctg gtgctgcgga tgtcgattgc
     1741 actgccgcgg gtgccggctc ggccaccggg gcgggctgtt ctggcgtcgc tgccgcgccg
     1801 cccttgtaga cccgcgcata gcgcgggccg aggctggtca agacctcgta gccgatggtg
     1861 ccgaaatggt gtgcaagctc gtcgaccgtg atgccctcgc caatcagcgt caccagatgg
     1921 ccgcgccgtg cggcgttatg ggcgagatcg gtgatgtcga ccgccatcag gtccatcgag
     1981 atgcggcccg ccaccgggca gcgcttgccg gcgaccacga cgtcggcgcc gcgggtgccg
     2041 tcattgctgc tgccggcgcg gaaatatccg tcggcatagc cgaccgagac gatcgcgagt
     2101 ttcgtgggcc gccgcgcggt ccaggtcccg ccgtaaccga cggtttcgcc cttctcgacg
     2161 ttgcggatct gcgcgatgcg cgccttcagt tcgaccaccg gctgcatcgg attgtcggcc
     2221 tcgggcgtcg gattgacgcc gtagagtgct gcccccggcc gcaccatgtc gaactggaat
     2281 tggcccccga gaaaaacgcc ggaggaattc gacagcgacg ccggcacgcc ggagaaaaga
     2341 ctggcgatct cgcggaagct cgcgagctgt ttggcgttga gcggatgatg gaggatttcg
     2401 gcgcaggcaa gatggctcat gaccagcgtg atgccgtgat cgccggcatt gattctggga
     2461 atgatgccct gcgcctccgc ggcggtgagg ccgagccggt tcatgccggt gtcgatatga
     2521 atggcggcac cgccggccca tccggagcgg cggcagaaca cgtcccattc ggcgagttca
     2581 ttgagatcgc ccatcaccgg cctggcgtcg atcctggcga acgcctcgcc ggaattctgg
     2641 aagaagccgc tgagcacata gatggccgcc gagggtattg ccgcgcgggc ggcgcgcgct
     2701 tcgtccagcg tggcgacaaa gaacgtcttg caaccggcgc cggcgagcgc gcgggcgacc
     2761 ggacccaccc cgcaaccata ggcgtccgcc ttgatcacac cggcgcattc ggccggcacc
     2821 gcggttttct ccagcttgcg ccagttggcg acgatggcgt caaggtcgac cgtgagcacg
     2881 ccgggtgcgg tcgcaagcgc ggttgcctgg tttgcttcag gcgacaacac ggcgctggtc
     2941 gggatcgatt tcgggtcggg aacgatgttc ataccgcaca ttacgcgcgg gggcccggcg
     3001 gttcaaccgg gcaggctgga tcagtacgtc tgcgccggca aatggctgtc ctgcgcgaga
     3061 tcgccgaacc gcgtaaactg gccctcaaac tgcagttcga ccgtgccggt cgggccgtgg
     3121 cgctgcttgc cgatgatgac ttcggccttg ccgtgcacca gcgacatgtc gagctgccat
     3181 ttttcgtatt cgggcgtgcc gacccgcggc tccttgttgg cgaggtaata ttcctcgcga
     3241 aacacgaaca tcacaacgtc ggcgtcctgt tcaatcgagc cggattcacg caggtcggac
     3301 agttgcgggc gtttgtcgtc gcggctttcg acctgacgcg acagctgcga cagcgcgatg
     3361 accggaacgt tcaattcctt ggccagcgcc ttcaggctgg tcgtgatctc ggtcacctcc
     3421 tgcacgcggt tgtcggaacg cttgcccgat ccctgcagca gctggatata gtcgaccacg
     3481 atcagatcga ggcctttttg gcgcttcaac cgccgcgcgc gcgcggtcag ctgcgaaatc
     3541 gaaaggccgc cggtttcgtc gacataaagc ggcagcgact gcaattcgat ggagtagtcg
     3601 cgaattttct cgaagtcggc ctcggtaatg ccgccgcgcc ggatggagct cgacgagatg
     3661 ctggtctgtt cggcgagaat acgcgtggca agctgttcgg ccgacatttc gcaggagaag
     3721 aagccgacga tgccgccatt gacggacttc atggtgccgt cggcctggac ttcggcgcga
     3781 tgcgccttgg ccacgttgta ggcgatgttg gtggcgagcg cggtcttgcc catgccgggg
     3841 cggccggcga cgatgatcag atcggagggt tgcaggccgc ccatcttggt atcgagatcg
     3901 cgcaggcccg ttgcaacgcc ggacaatttg ccgtcgcgct ggaacgcctt tgccgccatg
     3961 tcgacggcga cggtgagggc ctgcgcaaag cgctggaagc cgccgtcata gcggccggat
     4021 tcggccagtt cgtacagcct gcgctcggca tcctcgatct gcgcgcgcgg cgcgaaatcc
     4081 accggcgcat cgaaggcgac attgaccatg tcctcgccga tccggatcag gtcgcggcgc
     4141 agcgccagtt cgtagatcgt gcgtccataa tcctgcgcgt tgatgatggt ggtcgcttcc
     4201 gcggcgaggc gggcgaggta ttggccgacg gtcatgccgc cgatgtcggt ttcggccgga
     4261 acgaaggtct tcaacgtcac gggggttgcg accttgccca tccggatcag gctgccggcg
     4321 gtctcgtaga tggtctgatg gatcggctcg aagaaatgtt tcggctccag aaaatccgac
     4381 acccgataga acgcgtcgtt gttgaccagg atcgcgccca gcaggctctg ctccgcttcg
     4441 atgttgtgcg gtgcactccg ataggccgga gttcccgcgt cgggcgcgag cttgaggacg
     4501 ttcgaatcaa atggggccat ggttctcgag tgttcttggg actttgtcgg atacggggcg
     4561 ggggcggcga taaagcacca ctcccgggca tcacggaagc ggcaacgcgc cttatgcccg
     4621 attcacacac tgcaatgtgt taatcccgac tgggcagcgc gttggcgctt gacgagattc
     4681 gggcggacct cgggcgaggc ccgtcccatc gttcgaatat aaagagaaaa tttcggcgca
     4741 gcctgaactt ttcgccgcgc ggggacactc atcccaattg ggcgaggaat attccgaacc
     4801 gccatctcac cgctgaatca aatcaggagg agatagagca gggcaatcag ggcagccccg
     4861 acgccaacgg cgatcaaggc atagtcggtg ccgatgtcgc gctgcggatc gaacggtgat
     4921 gggtacgtct tctcgtccat ttcggagatt taggccaagc cggccggtcc atctgtgaag
     4981 cagatcacat ttggcggatt ttctacaatt gtatggtctt cgggaacggg ttggtgacat
     5041 cagggtttgt aatgtcacct tttgcggcaa ggaacgggcg atgggccagc aacttcagcc
     5101 atggcgatcg gaaaacggcc agactagatg gttgtcgatg ctgatcgacg ccatggaaaa
     5161 agtctcccgt ccggaattcc gcgcggtgcc ctgcgacccc tgggtgctgg cggcggtgag
     5221 ggcgcaactg gcgcgtccgg cctcgccata cgctcttggc gcatccaggt tcagcctccg
     5281 aaactagatt ctggaaacta gattttgcgt tagcggccta ttcgcctgca atttgcagcc
     5341 gcggccgctt gccgcgctcg atcccgcgca gtttcgcctc ctcatgcgcg atgtaatccc
     5401 gcgtcatcgg caccacgccc tggcgtttgg agagctggat ctggaaattc atcagatttt
     5461 gcttgcggaa cgacatttcc gacgacgcca ggtaaaattc ccacatccgc gcaaagcgct
     5521 cgtcgtagag ccgcaccgct tcctcccgcc gcgccatgaa acgctcgcgc cacgctttca
     5581 gcgtttcggc gtagtgcaaa cgcaggattt cgatgtcgca caccaacagt ccggcgcgct
     5641 cgatcgcggg catcacttcg gagagtgccg ggatgtagcc gccgggaaaa atgtatttcg
     5701 tgatccaggg gctggtgacg tcgggcccgt ccgaacggcc gatcgaatgc agcagcatga
     5761 cgccatcgtc cgtaagaagt tcggcacagc gcctgaaata ggtctcgtag aagtcgacgc
     5821 cgacatgttc gaacatgccc accgagacga tgcggtcgaa cggaccggaa atgtcgcgat
     5881 agtcctccag gaaaaacttc gccgacgccg ccagattttt ctcggccgcg cgggcgttcg
     5941 acacctccaa ttgttcggtc gacagcgtga tgccggtgat attggcgccg gtcatttcgg
     6001 ccagatacag cccaagtccg ccccagccgg acccaatgtc gagcacgcgg tcgccatgcc
     6061 cgatcaacag ttttgcggcg agatggcgct tcttggcgag ttgggcatcg tcaagggccg
     6121 cgtccggcgc ctcgaaataa gcgcagctat attgcttgtc ggcatcgaga aagagggaat
     6181 agagccgccc gtcgaggtcg taatggtgcg cgacgttgtt tttcgagcgg ccgggaggat
     6241 tgaactgctt ggcatgcctg gccagataac gcagccacca ttgcagtttg gcccatcgcg
     6301 gcaaaatttc aggctgatcc atcaggatcg ccagcgcatc cgcaatcgac cccttttcga
     6361 ccacgaaagt gccgtccatg tagacttcgc caagcgcaag ttcagggttg aggagaaccc
     6421 tgcgttccgc gtcagcggtc aggaatcgca cacagaccgg ctcgccggtt ccatcgccgc
     6481 agctaaacgt tgctccgctc gcggtcgtaa atgtcatcgc gccgcggcgg atgaactgct
     6541 gcaggaaata acgcaacaaa cgatgcatcg aaaccaccaa atgagcgatc ccgcagatgg
     6601 ccaaactacg atcggccgtc acactctcga agcacacggg acacaaccat cccgcgcacg
     6661 gctcccaagc gcctgtatga aattataagc gggatagttc cggtgcgcta ctgcgttgta
     6721 cacaaagcgg atattccaaa atatcgcgtt cacgccgttg cg
//
LOCUS       DASWGM010000652        13618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1575227,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000652 DASWGM010000000
VERSION     DASWGM010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575227"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1400
                     /locus_tag="VGA15_18315"
     CDS             291..1400
                     /locus_tag="VGA15_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589685.1"
                     /translation="MEGGMVTPAPALVRFTGIQKTYDGEHLVVKNLDLDIRKGEFVTL
                     LGPSGSGKTTTLMMLAGFEVPTHGEIYLADRPIKNMPPHKRDIGMVFQNYALFPHLTI
                     EENVAFPLSVRKINKAEAQERVRTALRMIKLENLAHRRPGQLSGGQQQRVALARALVF
                     NPQLVLMDEPLGALDKRLREQMQLEIKQLHDTMGITVVYVTHDQSEALTMSDRIAVFN
                     DGIVQQIDKPNALYEHPVNSFVANFIGENNVLAGTVENIEQGYCSVALASGGSVVAQA
                     VNVAGAGAATSLSVRPERIFILTDGAASEGPNRLPATVQNTIYLGDHALAVLQVAGNE
                     EFMVKLQPGAHGGLSHGQSVSITFRPEDCLALDPV"
     gene            1488..2537
                     /locus_tag="VGA15_18320"
     CDS             1488..2537
                     /locus_tag="VGA15_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589686.1"
                     /translation="MPKLKIALGFAATFTAGVVLATTAQARDLAVVSWGGAYQDAQKK
                     VYFEPFKKATGVPMNDESWDGGIGVLRAKVQGGAATWDVVQVESEELAVGCEEGLFEK
                     IEYSKIGGETAYLPPTVNSCGVGAIVYDFVLGYDKDKLKDGPKSWADFFDTKKYPGKR
                     SLRQGPKTTLEIALIADGVAPKDVYKVLATDEGIERAFKKLDTIKNDIVWWKAGAQPP
                     QLLASGEVTMTSVYNGRIDTANKNEKKNFGMVWDGALYTLDSWVILKGSPNKDAAYKF
                     LDFVGKAENQAKLSENIAYGTSNKDATAHLQPTVLKDLPTTPDNMKNAVEINVTFWLE
                     NIDRLTERFNKWATK"
     gene            2746..4008
                     /locus_tag="VGA15_18325"
     CDS             2746..4008
                     /locus_tag="VGA15_18325"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589687.1"
                     /translation="MTAAFFTGADTPTEVPLKRRLKRAERGRQFKALALVLPLLLFLL
                     FTFLGPLAGMLWRSVDDWEVRRVLPHTVAALADWDGKDVPDEKAYAALAGDIQAARAS
                     GTVAIASKRLNYALNGFRTILASTARNLKAAPEPGTAKETLGRINPAWGERATWAAIK
                     GASGPMTSFYLLAALDLTRNVDGAIVAAPPDQAIYRDVFARTFTISFGVTALCLILGF
                     PVAYLLATLPAGRSNLLMIFVLLPFWTSLLVRTCAWIVLLQREGVVNNSLRWLGIVDE
                     PLRLIYNRFGVYVAMTHVLLPFMILPLYSSMKAISPAYMRAAASLGASPVTAFLRVYL
                     PQTLPGIGAGCLLVFILALGYYITPALVGGAADQMISYFIALYTTETVNWGLASALGA
                     VLLLATLLLAAVYGKLVHGQQVTGGMKH"
     gene            4005..4835
                     /locus_tag="VGA15_18330"
     CDS             4005..4835
                     /locus_tag="VGA15_18330"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589688.1"
                     /translation="MSGNPSPRTPSQRIAWTTTIVASTLVFIFLMAPILAIMPLSFSS
                     GSYLTYPLPGLSLRWYEDFISSPRWMMSLRNSMIIGVASTVLSMVLGTLATLGLAQWK
                     SKFKPLVLAVVLSPVVVPGVITAVGLYFFFAPIGLTGSYLGLILAHTALATPFVVIPV
                     SATLQSFDMNLARAAASLGASPLYAFRRVILPLILPGLASGALFAFATSFDEVVVVLF
                     MAGPEQRTLPREMFSGIRENISPTITAAAVILTTVSVILLATMEGLRRRNERLKGNNA
                     "
     gene            complement(5246..6859)
                     /locus_tag="VGA15_18335"
     CDS             complement(5246..6859)
                     /locus_tag="VGA15_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018896751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9589689.1"
                     /translation="MAHDYIIIGGGSAGCVLANRLSEDPSISVLLIEAGGPDTNMLFR
                     MPAGFAKMTKGIASWGWSTVPQKHLNGRVIWYTQAKVVGGGSSINAQLYTRGNAKDYD
                     AWVTEAGCEGWSYRDVLPYFKRSEDNQRLVNAYHGYGGPLGVSYPVNPLPISFAFLRA
                     AQEAGIPFNDDFNGAVQDGIGHYQLTTRNAERSSTASAYLGPARSRQNLTVRLNTPTL
                     KIRVENRRAVGVEVGISGGIETLRAEREVILSSGAIGSPRLLQLSGIGPADALAAAGV
                     RVVHDLPGVGANMQDHLNLYAICECTGDHTYDRVAQPHRTLWAGLQYLLFKTGPVTST
                     LFETGGFWYADREARSPDIQFHLGLGSGIEAGVAAMRNPGVTLNSAFLRPRSRGTVRL
                     ANADPAAAPLIDPNYWSDTYDRECSLKGLQLARDILSQPALRPFVLAERLPSDGIRSE
                     EDLMEYAYRSCKTDHHPVGTCAMGSGENAVTTLDLKVRGMDGLRVVDASVMPFVPSCN
                     TNAPTIMVAEKASDVILGRKPLPAAFFDG"
     gene            complement(6872..8386)
                     /locus_tag="VGA15_18340"
     CDS             complement(6872..8386)
                     /locus_tag="VGA15_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9589690.1"
                     /translation="MSEPAALSRTGSPDVIIVGSGMGGATLAAGLAPTGARIVILERG
                     EQLKDTPETRDARAIFQRGFYRPTETWTDGAGQHFNPGNYYYLGGNSKFYGAVLIRYR
                     ACDFRPIAHAEGTTPGWPFDYDELEPWYTKAESLYRVCGALGDDPTEPRHSAPYPLPA
                     IPDEPAIAATRERLHRTGLHPASLPLGVDVEAWMKRGATPWDAFPDTRTGKMDAETCG
                     LAAALAHPNVTLQTGAKVQRLMMAPDGKHVEGVECDIGGERRTFNARLIVLSAGAVNS
                     AALLLRSSERGVANRNDMVGRHFMNHNASAVLAIDPRTVNDSIYQKTFGINDFYLDDG
                     AGGPPLGNIQLLGRVTGQILKANLRQVPEFALNWLSRHAVDFYAMSEDLPNPDSRVTL
                     DGAGIRLDWKRSNWASHLALVAKLRARLHAAGYPIVLARAFDRRTPSHQCGTVRIGLD
                     PAEAPLDPYCRAHDHPNLFVVDASFLPTSAAVNPALSIAAQALRVANHIASNDL"
     gene            complement(8471..9583)
                     /locus_tag="VGA15_18345"
     CDS             complement(8471..9583)
                     /locus_tag="VGA15_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016554383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589691.1"
                     /translation="MAFLEISGLRKRFGTLEVLKGIDLSLEKGGFLVLIGPSGCGKST
                     LLGTIAGLESISAGEICIEGQQINDLHPSKRDIAMVFQSYALYPNMTVAENMAFGMEM
                     RGVPKLEREKAVAEVAKTLQLEHLLKRRPSQLSGGQRQRVAMGRALVRQPRVFLFDEP
                     LSNLDAKLRVDMRVEIKRLHQRTGATIVYVTHDQIEAMTLATRIAVLKDGELQQVGTP
                     HEVYNRPANLFVADFMGSPSMNLLEGKVLRSNGAASVVIEHAGADPIAFPAPTSADVA
                     TLTEGARVIFGIRPEAVSDLEGADGNSKSIATFTGQVEIVEPAGSDTFVVTSIAGKEV
                     TARMRADSDIRVGQSHVFALNLDKAILFDPATTHRI"
     gene            complement(9587..10534)
                     /locus_tag="VGA15_18350"
     CDS             complement(9587..10534)
                     /locus_tag="VGA15_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006338966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9589692.1"
                     /translation="MSAADLVTAPPRRPTSGQSVITRVVIYGLLLFFAIIYLVPLIVM
                     VMASLKPLDEVTGGNMFALPKSPTFAPWIRAWGEASIGVSDTAGISGYFMNSIKMVVP
                     AVVISTLLGALNGYVLTKWRFPGHKLVFGMMLFACFIPFQSVIIPMAVILGLLGQIGN
                     QLIDATGFSFGFGNPTVNLVLCHVVYGLGFTTLFFRNYYEAFPTELVKAAMIDGAGFF
                     QIFRRVLLPNSTPIIIVTVIYQFTNIWNDFLFGSTFAAGDQSPMTVALNNLVNTSTGV
                     VEYNVNMAAAIIAAAPTLFVYVVAGRYFVRGLMAGAVKG"
     gene            complement(10531..11478)
                     /locus_tag="VGA15_18355"
     CDS             complement(10531..11478)
                     /locus_tag="VGA15_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016737540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9589693.1"
                     /translation="MSVTIAPIALVDRRSRPTIRERIQHFVPKLVLAPSFLLVLIFVY
                     GFNLWTVFLSFTNSKAFVSSKLIGLANYEKLWNWTFETDPPSSWYTAIVNIGIFGGLY
                     IVFCLALGLTLAILLDQKIRGEGILRPIYLYPLALSFIVTGTAWKLFLDPGIGLEKAV
                     HDWGWTSFKFDWVVNPRMMIYCVAIAGIWQTSGFVMAMFLAGLRGVDGEILKAAQIDG
                     ASAYATYRRIVIPIMRPVFLSAIIVLAHMAIKSYDLVLSVTGKNPGGAAELPSTFMYS
                     YTFTRNQMGVGSTSAVIMLMTIAAIMIPYLYSELREKRQ"
     gene            complement(11571..12836)
                     /locus_tag="VGA15_18360"
     CDS             complement(11571..12836)
                     /locus_tag="VGA15_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003578704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589694.1"
                     /translation="MKPRSFLSSSAMAAAVLGFAATAHAADVKEVQMLHWWTSGGEAA
                     ALNVLKQDLVKEGYVWKDVPVAGGGGAGAMTTLKAMVAAGNPPTASQILGYFALDYAE
                     AGKLGDITALATKDDWAKLVPTALQKFTTTNGKWDAVPVNIHSVNWIWLNKAVMEKIG
                     GTEPKTFDDFVALLDKAKAAGVIPLALGGQPWQEATMFDSVVASTGGIDFYKKAFIDL
                     DATALKSDTMKKAFDNLAKLRSYTDPNYAGRDWNLATAMVIKGDALVQVMGDWAKGEF
                     RAANKEAGKDFICYRFPGTDGAVIYNSDMFAIFNVPADRKAAQIAMADATMSKSFQSA
                     FNVIKGSVPARMDVPDTDFDMCGKKGIADVKSANASGKFLGSLAQNYAQPPAVAAAYD
                     DVVTKFFHGEIKTSDAAVTELIKAINNAK"
     gene            complement(13066..>13618)
                     /locus_tag="VGA15_18365"
     CDS             complement(13066..>13618)
                     /locus_tag="VGA15_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9589695.1"
                     /translation="LDMWRSWTRAGHRAFEALARLRTPLIVALTGHAFGGGLELAAAG
                     DFRIAERGVKLGLPETGLGMAPGWSGSQRLVRRFGPSVVRRMALAGQMFEGEAALALG
                     LIDEIAETGQGLARAEAMAADIAMHGPIAVQIVKAMINFAEGEDPDAPIEGLAGGLTA
                     MTQDLAEGVAAFRAKRAPTFRNS"
BASE COUNT         2608 a         4240 c         4255 g         2515 t
ORIGIN      
        1 aagcgcgcgg gcggtgggat caggggcgcc tgatcgagcc gacccgcagc gtcagcgcgc
       61 cgttcgctgc attgtcgacc acgctttccg gagaaaacac tgcacatggc cagtggcaaa
      121 gaagttttga ttgccgagtc cgatgttgac tgttgccgat ggtctttgcg gattggtcac
      181 gtactggacg tagcgctcgt gaaccgtcgt agagtcgaca ccgcatcttc tggaaatcct
      241 cgctgatgct aattccgacc ttgtgctcat caacgggaca ggaggaaggc atggaaggcg
      301 gcatggtcac gcccgcgcca gctttggtgc gttttaccgg tattcagaag acctacgacg
      361 gcgaacacct cgtggtgaag aacctcgacc tcgacatcag gaagggcgag ttcgtcaccc
      421 tgcttggccc ttcgggctcg ggcaagacga ccacgctgat gatgctggcg ggcttcgagg
      481 ttccgaccca cggcgagatc tacctcgctg accggcccat caagaacatg ccgccgcaca
      541 agcgcgacat cggcatggtc ttccagaact acgccctgtt tccgcacctg acgatcgagg
      601 agaacgtcgc cttcccgctg tccgttcgca agatcaacaa ggcggaggcg caggagcgcg
      661 tgcggacggc gctacgcatg atcaagctgg aaaacttggc gcaccggcgg ccggggcagc
      721 tgtccggcgg ccagcagcag cgcgtggcgc tggcccgcgc actggttttc aacccgcagc
      781 tcgtgctgat ggacgagccc ctgggcgcct tggacaagcg cctgcgggag caaatgcaac
      841 tagagatcaa gcaactgcac gatacaatgg gcatcaccgt cgtctacgtc acccacgacc
      901 agagcgaggc gctcaccatg tcggaccgca tcgccgtttt caacgacggc atcgtgcagc
      961 agatcgacaa gccgaacgca ctgtacgagc accccgtgaa cagcttcgtc gccaacttca
     1021 tcggcgagaa caacgtgctg gccggcaccg tcgaaaatat cgaacaggga tattgcagcg
     1081 tcgccctggc ctccggcggc tcggtcgtgg cccaggcggt caacgtggcc ggcgcaggcg
     1141 ccgccacgtc cctgtcggtg cggccggagc ggatcttcat cctaacggac ggggcggcca
     1201 gcgaggggcc gaaccgcctg ccggccacgg tgcagaacac catctacctc ggcgaccacg
     1261 cgctggccgt gctccaggtc gccggcaacg aggagttcat ggtcaagctt cagccgggcg
     1321 cgcatggagg cctgagccat ggccaaagcg tgtccatcac cttccgcccc gaagactgcc
     1381 tggcccttga tcccgtgtga tcgaaccctg cggtcggccc ccgagcgatc gggccgggac
     1441 gcatccagct ccaagacgca acctcacata aagaaggaac gaagacgatg ccgaagctta
     1501 agatagcgct gggattcgcc gcgacgttca ccgctggcgt tgtgctggcc acaacggcgc
     1561 aggcgcgtga cctggccgta gtgtcgtggg gcggtgcgta tcaggatgcc cagaagaagg
     1621 tttatttcga gccgttcaag aaggcgaccg gtgttccgat gaacgacgag tcctgggacg
     1681 gcggcatcgg tgtgctgcgc gccaaggtgc agggtggtgc tgccacctgg gatgtcgtcc
     1741 aggtcgagag cgaggaactg gcggttggct gcgaggaagg cctgttcgag aagatcgagt
     1801 actcgaagat cggtggcgag accgcctatc tgccgccgac cgtcaattcc tgcggcgtcg
     1861 gcgccatcgt gtacgatttc gttctcggct acgacaagga caagctgaag gacgggccga
     1921 agagctgggc cgacttcttc gacaccaaga agtatccggg caagcgcagc ctgcgtcagg
     1981 gtccgaagac cacgctggag attgccctca tcgccgacgg tgtcgcgccg aaggacgtct
     2041 acaaggtgct ggccaccgac gagggcatcg agcgcgcctt caagaagctg gacaccatca
     2101 agaacgacat tgtctggtgg aaggccggcg cccagccgcc gcagctgctc gcgtccggcg
     2161 aagtgacgat gacttcggtc tacaacggcc gcatcgacac cgcgaacaag aacgagaaga
     2221 agaacttcgg catggtatgg gacggggcgc tctacaccct cgacagctgg gtcatcctga
     2281 agggcagccc gaacaaggac gccgcctaca agttcttgga cttcgtcggc aaggcggaga
     2341 accaggccaa gctgtcggag aacattgcct acggtacctc gaacaaggac gctacggccc
     2401 acctgcagcc gaccgtgctg aaggatctgc cgaccacgcc ggacaacatg aagaacgccg
     2461 tggagatcaa cgttaccttc tggcttgaaa acatcgaccg tctgaccgag cgcttcaaca
     2521 agtgggcaac gaaataacgt tcccaagtcc gctcgactcc ggcaagacgg tgctgaagaa
     2581 accctcgcgc aaatgcttct gcaacggacg cggccgactg gccgcgtgac ctgagggcgg
     2641 tcggcgaagg ggtacaggca aaggactgac actttgatcg cctgtgctcc tcctcgcggc
     2701 gttccttgga ccgggtgaga tatcttgatg aaaaaggctc aacctatgac ggctgcgttc
     2761 tttaccggcg ccgatacgcc gaccgaggtg ccgctcaagc gccgcctgaa gcgggcggag
     2821 cgggggcgcc agttcaaggc attggcgctg gtcctgccgc ttctcctgtt cctgctcttc
     2881 accttcctgg gtcccctcgc cggtatgctc tggcgctcgg tcgacgactg ggaggtgcgt
     2941 cgcgtcctgc cccatacggt ggcggcgctg gccgactggg acggcaagga cgtaccagac
     3001 gaaaaggcct acgcggcgct ggcgggcgac atacaggcgg cgcgcgcctc cggcaccgtc
     3061 gccatcgcat ccaagcggct gaactacgca ctgaacggct tccgcaccat cctggccagc
     3121 accgcgcgca acctgaaagc cgcgccggag cccggcacgg ccaaggagac gctgggcagg
     3181 atcaatccgg cctggggcga acgcgccacc tgggcggcga tcaagggcgc cagcggccca
     3241 atgaccagct tctacctgct ggcggcgctc gacctgaccc ggaacgtgga cggcgcgatc
     3301 gtcgcggccc cgccggatca ggcgatctac cgcgacgtgt tcgcccgcac cttcaccatc
     3361 agcttcggcg tcacggcgct ttgcctgatc ctgggcttcc cggtcgccta cctgctggcg
     3421 acgctgccgg ccggccggtc gaacctgctg atgatcttcg tcttgctgcc tttctggact
     3481 tcgcttttgg tccggacctg cgcctggatc gtgctattgc agcgcgaggg cgtggtgaac
     3541 aacagcttgc gctggctcgg catcgtcgac gagccgctgc gcctgatcta caaccgcttc
     3601 ggcgtgtatg tggcgatgac ccacgttctg ctgcccttta tgatcctgcc gctgtacagc
     3661 agcatgaagg cgatctcgcc ggcttacatg cgtgccgccg cgtcgctcgg cgcgtcgccc
     3721 gtcaccgcct tcctgcgggt ctacctgccg cagacgctgc cgggtatcgg ggcgggatgc
     3781 ctgctcgtct tcatcctggc gctcggctac tacatcacgc cagcactcgt cggcggcgcc
     3841 gccgatcaaa tgatcagcta cttcatcgcc ctctacacaa ccgaaacggt caactggggc
     3901 ctggcctcgg cgctgggcgc ggtgctgctg ctcgccaccc tgctgctggc cgccgtctac
     3961 ggaaagctgg tccatgggca acaagtcaca ggaggaatga agcattgagc ggcaaccctt
     4021 ctccccgcac tcccagccag cgcatcgcgt ggaccacgac cattgttgcc tccacgctgg
     4081 tgtttatctt cctgatggcg ccaatcctgg cgatcatgcc gctgtccttc agctccggct
     4141 cctacctgac ctatccgctg ccgggcctct ccctgcgctg gtacgaggac ttcatcagct
     4201 ccccgcgctg gatgatgtcg ctgaggaaca gcatgatcat cggcgtcgcc tcgaccgtgc
     4261 tgtccatggt gctcggcacg ctggccacgc tgggtctggc gcagtggaag agcaagttca
     4321 agccgttagt gctggcggtc gtgctgtcgc cggtcgtcgt gccgggcgtc atcaccgcgg
     4381 tcggcctgta cttcttcttc gccccgatcg ggcttaccgg cagttacctg ggtttgatcc
     4441 tagcgcacac cgcgctggcg acgccctttg tggtgatccc ggtcagcgcg acgctgcaaa
     4501 gcttcgacat gaacctggcg cgcgccgcag cctcgctcgg cgcctcgccg ctctacgcct
     4561 tccgccgggt gatcctgccg ctgatcctgc ccggcttggc gtcgggcgcg ctgttcgcct
     4621 ttgcgaccag tttcgatgag gtggtggtcg tgctgttcat ggccgggccg gagcagcgca
     4681 ccctgccccg cgagatgttc agcggcatcc gcgagaacat cagcccgact atcacggcgg
     4741 cagcggtgat cctgacgacg gtgtcagtca tcctgctcgc caccatggaa ggcttgcgcc
     4801 gacgcaatga gcggctgaag ggcaacaacg cttgaccggc gtcgttatcc cttgcggcgg
     4861 acggtgagac cgtccgtccg ctggcttttc tcctgaaagc tgttgggcgt tcccaatccg
     4921 cgagcctcga tgagagcggg agcaagtttt ggcacgcttg tgcttaggca atcgggccgc
     4981 caggggaagc ctggcggccc cccccgcgcc tcgattgcca agaagatttg ggtcagatga
     5041 ttgcctcccg aacgcagcga atatcttcta agttgttgat tgttattgca acggcgggtg
     5101 tttccgccac gtaattctgg ccgacgaagg cctggcagct cggtttgttt ctcataactc
     5161 atgcgacgca cctgatctgg caggcgagcg gctcgacaac ctcatccatt cctcctcttt
     5221 cgtcggccgg ctcgcaagca gctctttagc cgtcaaaaaa agccgccggc agcggtttgc
     5281 gaccgaggat gacatcggac gccttctccg cgaccatgat agtcggtgcg ttcgtattgc
     5341 acgaagggac gaacggcatt accgaagcgt cgacgacacg caggccgtcc atgccgcgga
     5401 cctttagatc gagcgtcgtg acggcattct cgcccgagcc catcgcgcaa gtgccgacag
     5461 gatggtgatc cgtcttgcag gaccggtagg catattccat caggtcttcc tcactcctga
     5521 tgccgtcgct tggcagccgt tcggcgagca cgaatggtct cagcgctggc tggctgagga
     5581 tgtcgcgggc aagctgcagg cccttcagcg agcactcccg gtcgtacgta tctgaccagt
     5641 agttgggatc gatgagaggt gccgccgcgg gatccgcgtt tgccagccgc acggtgccgc
     5701 gagagcgtgg gcgcaggaac gccgagttca gtgtaacgcc gggattgcgc atcgccgcaa
     5761 cgcctgcctc gataccggat ccgagtccga gatggaattg aatatccggc gaacgcgcct
     5821 ccctgtccgc gtaccagaac ccccccgtct cgaacaatgt cgacgtgacg gggccggtct
     5881 tgaacagtag atattgcagc cccgcccaga gcgtgcgatg cggctgcgcc acgcgatcgt
     5941 aagtgtgatc gccggtgcat tcgcaaatcg cgtagagatt gagatgatcc tgcatattgg
     6001 caccaacacc tggcagatca tgaacgaccc gaaccccggc tgccgccagg gcgtcggcgg
     6061 gaccgatgcc ggagagctgc aatagccttg gtgagccgat ggcgccgctg gagagaataa
     6121 cctcgcgttc tgctcgaaga gtctcgatac cgccggagat gccgacctcc acgccgactg
     6181 cgcgccggtt ttcaacgcgt attttcagtg tgggcgtgtt cagtcttacc gtgaggttct
     6241 gtcgcgagcg tgccggtccg agataggcgc tcgccgtcga agaccgctcg gcattgcgcg
     6301 tcgtcaattg ataatgaccg atgccgtcct gcaccgcgcc gttgaagtca tcattgaacg
     6361 gaatgccggc ttcctgcgcg gcgcgcagga atgcgaagct gatcggcagg ggattgaccg
     6421 ggtaggaaac gcccaacggt ccgccataac cgtggtaagc attgacgagc cgctggttat
     6481 cctcggagcg cttgaaatag ggcaagacat cgcgatagga ccagccttcg cagcccgctt
     6541 ccgtcaccca ggcgtcatag tccttcgcat tgccacgcgt atagagctgg gcgttgatgg
     6601 acgaaccgcc gccgacgacc ttggcctgcg tataccagat gacgcgccca ttcagatgct
     6661 tctggggcac ggtgctccaa ccccagctgg cgatgccctt cgtcatcttg gcaaaaccag
     6721 ccggcatgcg aaacagcatg ttggtgtcgg gaccacctgc ctcgatgagc aggacactga
     6781 tcgacgggtc ttcactcagc cgattggcga gcacgcaacc ggcggagccg ccgccgatga
     6841 tgatgtagtc gtgggccatt gggtcctccg gtcaaagatc gttgctggca atgtggttcg
     6901 cgacgcgaag cgcctgcgcc gcgatggaca acgccgggtt gaccgccgcg gacgtcggca
     6961 ggaacgaggc gtcgaccacg aaaaggtttg gatggtcgtg agcacggcaa tagggatcga
     7021 gcggcgcctc cgccggatcc aggccgatgc gcacggtgcc gcactgatgc gaaggcgtgc
     7081 ggcgatcgaa cgcgcgcgca agcacgatcg ggtaacccgc agcatggagc cgcgcgcgca
     7141 atttcgcgac caaggcaagg tgcgaggccc agttgctgcg cttccaatcc agtcggatcc
     7201 ccgctccgtc cagcgtcacg cggctatcgg gattgggcag atcctcgctc atggcgtaga
     7261 agtccacggc gtgtcgactg agccagttca atgcgaattc ggggacctga cgcaggttgg
     7321 ctttgaggat ctgaccagtt acccgaccga gcagctggat gttgccgagc ggcggaccgc
     7381 ctgcgccgtc gtcgagatag aagtcattga taccgaatgt cttctgatag atggaatcat
     7441 tgaccgttcg cggatcgatc gccaatacgg ccgatgcatt gtgattcatg aaatgccggc
     7501 cgaccatgtc gtttcggttg gcaacgccgc gctcggagga gcgcagcaac agcgcagccg
     7561 aattgaccgc gccggcggac agcacgatca gcctggcgtt gaacgtgcgc cgctcgccgc
     7621 caatgtcgca ttcgacgcct tcaacatgtt tgccgtcggg cgccatcatc aggcgctgga
     7681 ctttcgcccc tgtttgcagc gtgacgttgg ggtgagccag cgcggcagca aggccgcagg
     7741 tctcggcgtc cattttcccg gtccgcgtat caggaaacgc gtcccagggc gtggccccgc
     7801 gtttcatcca ggcttcgacg tcgacaccga gcgggagcga cgccgggtgc agtcctgttc
     7861 tgtgcagccg ttcgcgggtt gccgcaatgg ccggttcgtc agggatggcc ggcagaggat
     7921 agggcgcgga atgccgtggc tccgtggggt cgtcaccgag cgcgccgcag acgcgataca
     7981 agctctcggc ctttgtatac caaggctcca attcgtcgta gtcgaagggc cagcctgggg
     8041 tagtcccttc cgcatgcgcg atcggccgga agtcgcacgc gcggtatcgg atcaggaccg
     8101 cgccgtaaaa cttgctgttt ccacccaaat aataatagtt gccggggttg aagtgttgac
     8161 ccgctccgtc tgtccaggtc tcggttgggc gataaaaacc gcgttggaag atggcccgcg
     8221 cgtcgcgcgt ttcgggcgta tctttgagct gttcgccgcg ttcgagaata acgatgcgag
     8281 cccccgtcgg cgcgagcccg gcggcgagcg tcgcgccacc catgcctgat ccgacgatga
     8341 tcacgtcggg gcttccggta cggctcaagg cggctggctc agacatcgaa cctcgcgaca
     8401 acacgctcgc ggcagtggcc aactgtcccc attgcccggt cggcaacacg gaccggtggc
     8461 ggcagatggt ttaaattcga tgcgtcgtag ccgggtcgaa caagatggcc ttgtctaggt
     8521 tcagggcaaa cacatggctc tgcccgacgc gaatgtcgga atccgcccgc atccgcgccg
     8581 tgacctcctt gccggcgatc gaggtgacga cgaatgtgtc cgatccggcg ggctcgacga
     8641 tttcgacctg gcctgtgaag gtcgcgatgc tcttggagtt gccgtccgcg ccttctaggt
     8701 cgctgacagc ttcaggacga atgccgaaga tgacccttgc accctccgtg agcgttgcca
     8761 cgtccgcgct cgtgggagca ggaaacgcga tcgggtccgc tccggcgtgc tcgatcacga
     8821 ccgaggcagc gccgttcgat cgcaagactt tgccttccag caggttcatg gacggcgagc
     8881 ccatgaaatc ggcgacgaac agatttgcgg gcctgttgta gacttcgtgc ggcgtgccga
     8941 cctgctgaag ctcgccgtcc ttcaggacgg cgatgcgcgt cgcaagcgtc atggcctcga
     9001 tctgatcgtg cgtgacatag acgatggtcg cgccagttcg ctggtgcagg cgcttgatct
     9061 caacacgcat gtcgacgcgc agcttggcgt cgagattgga cagcggctcg tcgaacagga
     9121 agacacgcgg ctggcgaacc aatgcgcgcc ccatggcgac gcgctggcgc tggccgcctg
     9181 aaagctggct cggacggcgc ttgagaagat gctccaattg gagggtcttg gcgacctcgg
     9241 cgacggcctt ttcgcgctca agtttcggca cgccgcgcat ttccatgccg aacgccatgt
     9301 tctcggcgac cgtcatgttg ggataaagcg cgtaggactg aaacaccatc gcgatgtcgc
     9361 gcttcgacgg gtgcagatca ttgatctgct gaccttcgat gcaaatttcg ccggcactga
     9421 tcgattccag gcccgcgatc gtgccaagca gggtcgactt gccacacccc gaggggccga
     9481 tgagcaccag aaagccgccc ttctccaggc tgagatcgat gcccttcagg acctccagcg
     9541 tgccaaatcg cttgcgaagc ccgcttatct cgaggaaagc catgttctat cctttcaccg
     9601 cgccagccat cagcccgcgc acgaagtagc ggcctgccac gacatagacg aacagagtgg
     9661 gcgcggcggc gatgatggcc gcagccatgt tgacgttgta ttcgacgacc ccggtcgagg
     9721 tattgacgag gttgttcagt gcgaccgtca tcggagactg gtcgccggca gcgaaggtcg
     9781 acccgaacag gaagtcgttc cagatattgg tgaactggta gatcaccgtg acgatgatga
     9841 tcggggtcga gttcggcagc aggacgcgtc ggaagatctg gaagaatccg gcgccgtcga
     9901 tcatggccgc cttcaccagt tcggtcggaa acgcctcgta gtagttgcgg aaaaacaacg
     9961 tcgtgaagcc gaggccatag acgacatggc aaagcaccag gttgaccgtc ggattgccga
    10021 aaccgaacga aaagccggtg gcgtcaatga gctgattgcc gatctggccc aacagtccca
    10081 ggatcaccgc catcggaatg atgacggatt ggaacgggat gaagcaggcg aacagcatca
    10141 tgccgaacac cagcttgtgt cccgggaagc gccatttggt gaggacatag ccgttcagtg
    10201 cgccgagcag cgtcgagatc acgaccgccg gcaccaccat cttgatcgag ttcatgaaat
    10261 agccgctgat cccggcggta tcggagacac cgatgctggc ttcgccccag gccctgatcc
    10321 agggggcaaa ggtcggcgat ttcggcagcg cgaacatatt gccgccggtg acttcgtcga
    10381 gcggcttcaa cgacgccatc accatcacga taagcggcac cagataaatg atcgcgaaaa
    10441 acagcagcaa cccatagatc accacacggg tgatgacgga ttggcccgac gtgggacgac
    10501 gcggcggagc ggtgacgaga tccgcggcgc tcattggcgt ttctcccgca attccgaata
    10561 gaggtaagga atcatgatcg cggcgatcgt catcagcatg atgaccgcgc tggtggagcc
    10621 cacccccatt tgattgcgcg tgaaggtgta ggaatacatg aaggtcgatg ggagctcggc
    10681 ggccccgccc gggttcttgc cggtgaccga caagacgagg tcgtaggatt tgatcgccat
    10741 gtgggcgagc acgatgatgg cggagagaaa caccggccgc atgatcggga tcacgatccg
    10801 ccgataggtc gcataggcgg aggcgccgtc gatttgcgcg gccttcagga tctcaccgtc
    10861 cacgccgcgg aggccggcga gaaacatcgc catcacgaag ccggaggtct gccagatgcc
    10921 cgcaatcgcg acgcaataga tcatcatgcg gggattgacg acccaatcga acttgaaact
    10981 ggtccagccc caatcgtgga cagccttctc gagcccgatg cccggatcga gaaagagctt
    11041 ccacgccgtg ccggtgacga tgaacgacag cgccagcgga tagagataga tcgggcgcag
    11101 aatgccctcg ccgcggatct tctgatcgag caggatcgcc agtgtcaggc ccagcgcgag
    11161 acaaaagacg atatagagcc cgccgaagat gccgatattg acaatggcgg tgtaccagct
    11221 tgacggcggg tcggtctcga acgtccagtt ccaaagcttt tcgtaattgg cgaggccgat
    11281 cagcttggag ctcacgaacg cctttgaatt cgtaaaggac agaaatacgg tccagagatt
    11341 gaagccatag acgaaaatca agacgagcag gaacgacggc gcgagaacga gcttcggcac
    11401 gaaatgctga atacgttcac gtatcgtggg gcgcgaccgg cgatcgacta aggcgattgg
    11461 agcgatggtt acagacatgg acacctcccg ttagccggac cgcacgacac aacgaaccgt
    11521 tacccacgcc tctctcggat cacggagaga ggcgtgagca agaaaaacgc ctatttggcg
    11581 ttgtttatcg ctttgatgag ctcggtcaca gccgcatcag aagtcttgat ctcgccgtgg
    11641 aagaacttgg tgacgacatc gtcatacgca gcggcaacag ccgggggctg cgcgtagttc
    11701 tgcgccagcg atccgaggaa cttgccgctg gcgttggcgg atttgacgtc ggcgatgccc
    11761 ttcttgccgc acatgtcgaa gtccgtatcc ggcacatcca tgcgagccgg caccgagccc
    11821 ttgatgacat tgaaggcgga ttggaagctc ttgctcatgg tcgcatcggc catggcgatt
    11881 tgtgcggcct tgcgatccgc gggcacattg aagatggcga acatgtccga gttgtagatc
    11941 actgcaccgt cggttcccgg gaagcggtaa cagatgaagt ccttgccggc ttccttgttg
    12001 gccgccctga actcgccctt ggcccagtcg cccatcacct gcaccagcgc gtcgcccttg
    12061 atcaccatcg ccgtggccag gttccagtcg cggccggcgt aattcgggtc ggtgtaggag
    12121 cgaagcttgg cgaggttgtc gaacgccttc ttcatggtgt ccgacttcag ggcagtcgcg
    12181 tcgagatcga tgaacgcctt cttgtagaaa tcgataccgc cggtggaggc gacgacggag
    12241 tcgaacatgg tggcttcctg ccaaggctga ccgccaagcg cgagcggaat gacgcccgcc
    12301 gccttcgcct tgtcgagcag ggcaacgaaa tcatcgaaag tcttgggttc cgtgccgccg
    12361 atcttctcca tcaccgcctt gttgagccag atccagttga cggagtggat attgaccggc
    12421 acggcatccc acttgccatt ggtggttgtg aacttctgca acgccgtcgg gaccagcttg
    12481 gcccagtcgt ccttggttgc aagcgccgtg atatcgccga gcttgccggc ttccgcatag
    12541 tcgagcgcga aatagccgag aatctgggac gcggtcggcg gattgccggc ggcgaccatt
    12601 gccttcagcg tcgtcatggc gcctgcgccg ccgccaccgg caacgggcac gtccttccaa
    12661 acatatccct ccttgacgag gtcttgcttc aggacgttca gcgcggcggc ctcgccgcct
    12721 gaggtccacc agtgcagcat ctgcacttct ttgacgtcgg cggcgtgtgc ggtcgcggca
    12781 aagccgagga ccgcagcggc catcgcgcta gatgataaaa agctacgagg cttcattgtt
    12841 tttaccttcc aaggcatact ctctcccacc ggattcggtc cggcgccgtt tccagaagcc
    12901 ttgttgactt ccgatctttt tggttcagct caggtccgca ggagccacca gctcgtgcgc
    12961 ggacctttgt gaacgtggaa cgtttttatc ctcggtatag tcggcgacag cgcagagtcc
    13021 aagacctgat tgaccgtatc catcgaacgg caattcaacc tcctctcagc tgttgcgaaa
    13081 cgtcggcgcg cgcttggcac gaaacgccgc gacgccttcg gccaggtctt gtgtcatcgc
    13141 cgtcagcccg cccgcgagcc cctcgatcgg tgcgtccggg tcctcgcctt cggcaaagtt
    13201 gatcatcgcc ttgacgatct ggacggcgat aggaccgtgc atggcgatat cggcagccat
    13261 ggcctctgcg cgcgccagtc cctgaccggt ttctgcgatc tcatcgatga ggccgagcgc
    13321 aagcgcggcc tcgccctcga acatctgtcc ggccagcgcc atgcggcgaa cgaccgatgg
    13381 gccgaaacga cggacgagcc gctgcgaccc cgaccagccc ggcgccatgc cgaggcccgt
    13441 ctcgggcaaa cccaatttga cgccgcgctc ggcaatacga aaatcgccgg cagccgccag
    13501 ttccaggccg ccaccgaagg cgtgacccgt cagcgcaacg atcaagggcg tacgcagccg
    13561 cgcgagcgct tcaaacgcgc gatgaccggc acgtgtccag cttcgccaca tgtccaac
//
LOCUS       DASWGM010000653         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_912827,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000653 DASWGM010000000
VERSION     DASWGM010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912827"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VGA15_18370"
     CDS             complement(<1..677)
                     /locus_tag="VGA15_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9589696.1"
                     /translation="MDIMTGVGLVFGIIVVAVMVFMGGDLHMFVSEHAVIIIFGGSIA
                     ATMIRFPLSAILHGLPLGAKFAFTMSRLSARDLVDELARIAEIARKQGPVGLEKVETD
                     EPFLAKGIRYVADGYDLEFIRDNLERDRDNFLMHLDEGGKIYRAIGDCAPAFGMIGTL
                     IGMVQMFANMTDPSKLGPFMATALLATLYGALVANLFCLPIADKLHGKLLDEETNRTL
                     IIDGILM"
     gene            complement(1003..1830)
                     /locus_tag="VGA15_18375"
     CDS             complement(1003..1830)
                     /locus_tag="VGA15_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9589697.1"
                     /translation="MKMDGKTVLITGSTDGVGRFVAAKLAAAGANVLIHGRDQARAKT
                     LADEIRGQGHIEPIFHQADLSSLAGARQLAAAVLAGHKRLDVFISNAGIGSRSLGSER
                     QTSADGHELRFAVNYLSGFLLAHLLLPLLKTSAPARIVNVASLGQHPLDFDDVMLTKG
                     YNGNRAYAQSKLAQIMFTIDLARELEGSGVTVNALHPATYMNTTMVREGGITPISTVE
                     QGGEAILHLAAGDDVAGKSGLFFNGMQQAQANPQAYDAGARQRLRALSLELTGLSAA"
BASE COUNT          374 a          602 c          599 g          338 t
ORIGIN      
        1 atcatcagga tgccgtcgat gatcagcgtc cggttggtct cctcgtcgag caacttgcca
       61 tgcagcttgt cggcgatcgg caggcagaac aggttcgcga ccagcgcgcc gtagagcgtc
      121 gccagcaacg cggtcgccat gaatggaccg agctttgaag gatcggtcat gttggcgaac
      181 atctgcacca tgccgatcaa cgtgccgatc atgccgaacg ccggcgcgca gtcgccgatg
      241 gcgcggtaga tcttcccgcc ctcgtccaga tgcatcagaa aattgtcgcg gtcccgttcg
      301 agattgtcac ggatgaattc gagatcgtag ccgtcggcga catagcggat tcccttggcg
      361 agaaacggct cgtcggtctc gactttttcg aggccgaccg gcccctgctt gcgggcgatt
      421 tcagcgatgc gggcgagttc gtcgacgaga tcgcgcgcgg acagcctgct catcgtgaaa
      481 gcaaatttcg caccgagcgg cagaccgtgc agaatagcac tgagcggaaa cctgatcatg
      541 gtggcggcaa tcgaaccgcc gaagatgatg atgaccgcat gttcgctgac gaacatgtgg
      601 agatcgccgc ccatgaacac catcaccgcc acgacaatga tgccgaacac gagcccaacg
      661 cccgtcatga tatccatggg aactccaacg cgtaggcgaa cgaccggccg ttctggacgg
      721 agacgaggcc catcccgagc cccgcaacca gaaccctacg tccccgccat taaagacgcg
      781 taaagcttaa accgtcgggg gcccgtacga accggccgca accttgatgc gcgcggcggc
      841 caaagccgcg aagcggccaa tggatcagcg tcccggcgtg atttcaacgt tctgctaacc
      901 ataatcggcg tgcctctttc cggcagcggc cggtgccggc ggggcccggc cgctccgagg
      961 gacacgatgt ccgggccagc gggcggagcc tcgtcggttt cgtcaggcgg ccgacaggcc
     1021 ggtgagttcg aggctgagcg cacgcaatcg ctggcgcgcg cccgcatcat aggcctgcgg
     1081 attggcctga gcctgctgca tgccgttgaa aaacagcccg ctcttgccgg ccacatcgtc
     1141 gccggcggca agatggagga tcgcctcccc accctgctcc accgtagaga tcggcgtgat
     1201 gccgccttcg cggaccatcg tggtgttcat ataggtcgcg ggatgcagcg cattcacggt
     1261 cacgcccgat ccctcgagtt cacgtgccag gtcgatcgtg aacatgatct gggccagctt
     1321 gctctgcgca taggcgcggt tgccgttata gcctttggtc agcatcacgt catcgaaatc
     1381 gagcgggtgc tggccgagcg aggcgacatt cacgatacgc gcgggcgcgc ttgtcttcaa
     1441 caagggcagc agcagatgag cgagcagaaa accggacagg tagttcacgg caaagcgcag
     1501 ctcgtgtcca tccgcgctgg tttgccgctc agacccaagc gaccgcgatc cgatcccggc
     1561 attgctgatg aagacgtcga gccgcttatg cccggcgagc accgcggccg cgagctgccg
     1621 cgctcccgca agcgaagaca ggtcggcctg atggaagatc ggctcgatgt gtccctggcc
     1681 acgtatctca tcggcaaggg ttttggcgcg cgcctggtcg cggccgtgga tcaggacgtt
     1741 ggcgccggcc gcggccagtt tggcggcgac aaagcggccg acgccatcgg tcgagcccgt
     1801 gatcaaaacc gtcttgccgt ccattttcat cgtgaagcgc tcctacgcgt attccgcaaa
     1861 agcacgccct cggacttgat ccgggggtgg gtaccggttt tgcgaataga ata
//
LOCUS       DASWGM010000654         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1871850,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000654 DASWGM010000000
VERSION     DASWGM010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871850"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VGA15_18380"
     CDS             complement(<1..1578)
                     /locus_tag="VGA15_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9589698.1"
                     /translation="MRLIRCLAPLALGLMIVVAAAPARAIDAVSVRGDAPAIDLTAVL
                     DHQHSDTDRIQVSTAPGTDGIVRRIEVRAREGGQFWVVFALANNTDDQLDRLIVAPHY
                     RIVSSGLLWPDLGLSRIATITPSTGDRPDRQESATADIFRITLDPGAVITFVAELRTD
                     KLPQLYLWEPDAYKDKVNSFTLYQGIVIGISGLLALVLTILFVVKGSVMFPAAAALAW
                     AVLVYIGVDFGFWGKVLDMSNNAERIWRAAGEAVLAATLLVFLFAYLNLSRWHVRYSH
                     ITVGWLVFLGSLVALALFDPPVASGIARMSLVLIAFAGFALIVYLSTHGFDRAVLLIP
                     TWFLLLVWVIAAGMTVGGSVTNDIVGPALLGGLVLIVMLIGFTVMQHAFAGGGATTGM
                     VSDVERRALALTGSGDLIWDWDVSADKVFTSPETESLLGLKRGTLEGPAAKWLEVLHP
                     LDQDRFRAALDSVLDQRRGRLVQDFRLRTPDGHFMWFALKARPVVGSDGEVSRVVGTL
                     TDVTESKNAEERLLHDSV"
     gene            complement(1716..>1808)
                     /locus_tag="VGA15_18385"
     CDS             complement(1716..>1808)
                     /locus_tag="VGA15_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9589699.1"
                     /translation="MDSTFPLEKAADAHRRMETSEHIGKIVLVV"
BASE COUNT          369 a          563 c          598 g          278 t
ORIGIN      
        1 caccgaatcg tgcagcaggc gttcctcggc gttcttgctt tcggtgacgt cggtgagcgt
       61 gccgaccacg cgcgagactt cgccgtcgga gccgaccacc ggacgcgcct tcagcgcgaa
      121 ccacatgaag tggccatcgg gtgtgcgcag ccggaaatcc tgcaccagcc ggccgcggcg
      181 ctggtcgagc acgctgtcga gggcggcgcg gaaacgatcc tgatcgagcg gatgcagcac
      241 ttcgagccat ttcgcggcgg ggccttccag cgtgccgcgt ttcaggccga gcaggctttc
      301 agtctcgggg ctggtgaaga ccttgtcggc cgagacatcc cagtcccaga tcaggtcgcc
      361 cgagccggtc agcgccagcg cccggcgctc gacgtcggag accatgccgg tggtggcgcc
      421 gccgccggcg aaggcgtgct gcatcaccgt aaatccgatc agcatcacga tcagcacgag
      481 gccgccgagc agcgccgggc cgacgatgtc gttggtgacg ctgccgccga ccgtcatgcc
      541 ggccgcgatc acccagacca gcagcaggaa ccaggtcggg atcagcagca ccgcgcggtc
      601 gaacccatgg gtcgacagat agacgatcag cgcgaagcct gcgaacgcga tcagcaccag
      661 cgacatccgc gcgatccccg aggcgaccgg ggggtcgaac aatgccagcg ccaccagcga
      721 gccgagaaag accagccagc cgacggtgat atgggaataa cgcacatgcc agcggctgag
      781 attgagatag gcaaacagaa acaccaatag cgtcgccgcc aggaccgctt cacccgccgc
      841 gcgccagatc cgctcggcat tgttcgacat gtcgagcacc ttgccccaga agccgaaatc
      901 gacgccgata tagaccagca ccgcccaggc caatgctgcg gcggcgggga acatgacgct
      961 gcccttgacc acgaacagga tggtgagcac cagcgccagc aatccggaaa tgccgatcac
     1021 gattccctga tacagcgtga acgagttgac cttgtccttg taggcgtcgg gctcccacag
     1081 atagagctgc ggcagcttgt cggttctaag ctcggcgaca aaggtgatga cggcgccggg
     1141 atcgagcgtg atgcggaaaa tgtccgcggt ggcgctttcc tggcggtcgg gacggtcgcc
     1201 ggtcgagggc gtgatggtcg cgatgcgcga caatccaagg tccggccaca acagacccga
     1261 cgatacgatg cgatagtgag gagcgacgat cagacgatcg agctggtcgt cggtgttgtt
     1321 ggcgagcgca aacaccaccc agaactggcc gccttcgcgg gcgcggactt cgatgcggcg
     1381 gacgatgccg tcggtgcccg gtgcagtgga aacctgaatg cggtcggtgt cgctgtgctg
     1441 atgatcgagc accgcggtga gatcgatcgc gggcgcgtca ccgcggacgc tgacggcgtc
     1501 gatagcacgc gctggcgccg cggcgacaac aatcatgagg cccagcgcga ggggcgcgag
     1561 gcacctgatc agacgcaatg tcagttctcc gcgttcgaca aaggtgaaga ggcaagaccc
     1621 gttccgaatg cggcgagcgc gttcggcatc gtgcgataaa tgacatgaaa gcgagggaga
     1681 tcaaagagtt tccggttggc cgtcattcac gaccgctaga caaccaacac aattttgcca
     1741 atatgttcgc tggtctccat gcggcggtgc gcgtccgcgg ctttttccag cgggaatgtg
     1801 ctgtccat
//
LOCUS       DASWGM010000655         4222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1298570,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000655 DASWGM010000000
VERSION     DASWGM010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298570"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VGA15_18390"
     CDS             complement(<1..1302)
                     /locus_tag="VGA15_18390"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX9589700.1"
                     /translation="MFKRILIANRGEIACRVIKTARRMGIETVAVYSEADRDALHVEM
                     ADKAVLIGPPAAAESYLVMEKIVEACRKTGAQAVHPGYGFLSEREAFPRVLSEAGIVF
                     IGPNASAIAAMGDKIESKKAAAKADVSTVPGYLGVITDERHAVKIADAIGYPVMIKAS
                     AGGGGKGMRIAHSTSEVAEGFGLAKAEAKASFGDDRVFIEKFIVDPRHIEIQVLGDKH
                     GNVIYLGERECSIQRRNQKVIEEAPSPLLDETTRRKMGEQAVALAKAVKYDSAGTVEF
                     VAGQDKSFYFLEMNTRLQVEHPVTELITGIDLVEQMIRVAAGEKLALKQKDVTLTGWA
                     VETRVYAEDPFRNFLPSIGRLVKYRPPVESSIDGVTVRNDTGVQEGGEISIYYDPMIA
                     KLVTHAPSRAAAIEAQATALDSFYIDGIRHNIPFLSALMLHP"
     gene            1571..1957
                     /locus_tag="VGA15_18395"
     CDS             1571..1957
                     /locus_tag="VGA15_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589701.1"
                     /translation="MAQLALFAEPNADPEGLRYQPEFISRTTEAALIGHILELPLQPF
                     QFGAYEGKRRVASFGFRYDYTAKRLQEAEPLPAWLIPAISSVENFGDLPPGSVRQVLC
                     AEYDVGVGIGWHRDKKMLGGGILEGV"
     gene            2053..3897
                     /locus_tag="VGA15_18400"
     CDS             2053..3897
                     /locus_tag="VGA15_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9589702.1"
                     /translation="MRRSILLLSAGMVVLSAGMASAQNSAPHNLILFVPDGLRALKVT
                     PETAPAMAEVRDKGVNFKNPHSLFPTFTMANGSAMATGHHLGDTGTFSNTIYTGFSSA
                     PAGDTVVPFIENDAVLGDVDEHFGGDYLNEETILKMARTKGFSTAAIGKVGPTLIFDH
                     TDRADKPGLHSIVIDDSTGGKNGVPLSEEMKAALTKANLPLAAPSRGDNGKAGDAKTP
                     GTVVPNTAQQAYFADVASRIVLPLFKARAKPFVLVFWSRDPDGTQHNTGDSLNTITPG
                     INGPTSLAGIKNADNNLAQLREALDELGLSATTNVMVAADHGFSTISKESKTSPSAKA
                     TYEDTPKDFLPMGFLAIDLAKALDLPLFDPNDKNARIAENAHPKAGNGVLGQDPAKPD
                     LVVATNGGSDLIYLPKKDKKLAGRAVKALLEQDYVSGIFVDDALGRYPGALPLSALNL
                     KGKAVTPQPSIVVNFRSYASGCDEPTTCSVEVADTVLRQGQGMHGGFSRGDTMNFMAA
                     VGPDFKAGYVDALPVSNADVGMTIAQLLDLHAAAVGGLTGRVMSEALPNGIVSKFADG
                     TISSKPDPNGLQTVVKFQRVLSQRYFDVAGFPGRTLGLEAGKQKTAGK"
     gene            complement(3935..>4222)
                     /locus_tag="VGA15_18405"
     CDS             complement(3935..>4222)
                     /locus_tag="VGA15_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589703.1"
                     /translation="GRFDDLLAKAVVHHAASESGLPVPSRTIALSPDTAIESWAPARA
                     AGVNSEVLDWIASQIRGKRRSNRTLMRMVATIVGAAALPFSQLPKVFDIGM"
BASE COUNT          839 a         1314 c         1268 g          801 t
ORIGIN      
        1 cggatgaagc atcaacgccg acagaaacgg aatgttatgg cggataccgt cgatgtagaa
       61 tgaatcgagc gcggtcgcct gcgcctcgat cgcggcggcg cgcgacggcg cgtgggtcac
      121 gagcttggcg atcatcggat cataatagat cgagatctcg ccgccctcct gcacgccggt
      181 atcgttgcga acggtaacgc cgtcgatgct gctctcgacc ggcgggcgat attttaccag
      241 ccggccgatc gagggcagga aattgcggaa cggatcttcc gcatagaccc gcgtttccac
      301 cgcccatccg gtcagcgtga cgtccttctg tttcagcgcc agcttctcgc cggcggcgac
      361 gcggatcatc tgctcgacca ggtcgatgcc ggtgatcagt tcggtcacgg gatgctcgac
      421 ctgcaggcgg gtattcatct ccaggaagta aaaactcttg tcctggcccg cgacaaactc
      481 aaccgtacca gcggaatcgt atttcacggc tttcgcaagc gccaccgcct gctcgcccat
      541 cttgcggcgg gtggtctcgt cgagcagcgg cgacggcgct tcctcgatca ccttctgatt
      601 gcggcgctgg atcgagcatt cgcgctcgcc gagataaatc acgttgccat gcttgtcgcc
      661 gagcacctga atttcaatgt ggcggggatc gacgatgaat ttttcgatga atacccggtc
      721 gtcgccgaac gacgctttgg cttccgcctt ggcgaggcca aagccttcgg caacttccga
      781 ggtcgaatgc gcaatccgca tccccttgcc gccgccgccg gcggaggcct tgatcatcac
      841 gggatagccg atcgcgtcgg cgatcttcac cgcatgcctt tcgtccgtga tgacgccaag
      901 gtagcccggc acggtcgaga catcggcctt cgcggctgcc ttcttggatt caatcttgtc
      961 gcccatggcg gcgatagcgc tcgcgttggg tccgatgaaa acgatacccg cctcgctcag
     1021 cacgcgcggg aatgcttcgc gctcggacag gaagccgtag cccggatgca cggcttgcgc
     1081 gccggtcttg cggcaggcct cgacgatctt ctccatcacg agatagcttt cggccgcggc
     1141 cggtggtccg atcaaaactg ccttgtcggc catttcgaca tggagtgcgt cgcggtcggc
     1201 ctcggaatag acggccacgg tctctattcc catccggcgg gcagtcttga tgacccggca
     1261 ggcgatctcg ccgcggttgg cgatcagaat tcgtttgaac attttcttaa ctgcagcctt
     1321 tgggcgggat ccggagccac aaactggcac tcaggggacg ggcgtaacaa ttccgtcgta
     1381 cagcaaaacc aaacagaggc aacggcttat atgccgaaaa tccgcttgtc agggtagata
     1441 ttcccgcgcc cttcagtcag gctttgcagc gagggtgcgg ggccccatcc tccgtatcgg
     1501 cggcccttgc ggaacggttc cgggaaagga gcgttctccg aaacgttttt tcgaaaaagg
     1561 atttaaccaa atggcgcagc ttgcgctttt tgcagagcca aacgcagatc cggaaggact
     1621 ccgctatcaa cccgaattca tatccaggac aaccgaggcg gcacttatcg gtcacatcct
     1681 ggaattgccg ttacagccat tccagttcgg cgcctacgaa ggaaaaaggc gggttgcttc
     1741 gttcggcttc cgctacgatt acaccgcaaa gagacttcaa gaagccgagc cactgcccgc
     1801 atggctgata ccggccatca gttcggttga aaatttcggc gatctgccgc ccggatccgt
     1861 ccggcaagtt ctgtgtgccg agtacgatgt cggcgtgggc attggatggc accgcgacaa
     1921 gaagatgctg ggcgggggga ttttggaggg cgtataattg ttgggccagc ccatgtaaca
     1981 aatctgtcat acagcaaaac taaagaggca acggctgata cgccggaatc tgcttttcag
     2041 ggggagatct tcatgcgccg ctcgatccta ttgctgtccg ctggcatggt cgtgctgtcc
     2101 gccggcatgg catctgcgca gaacagcgcg ccccataatc tgatcctgtt cgtgccggat
     2161 ggattgcgcg ccttgaaggt cacgcccgaa accgcgccgg cgatggcgga agtccgcgac
     2221 aagggcgtca atttcaaaaa tccacattcg ctgttcccga ccttcaccat ggcgaacgga
     2281 tcggcgatgg cgaccgggca tcacctcggc gataccggaa cgttcagcaa cacgatctat
     2341 accggttttt cgtccgcgcc cgccggcgac accgtcgtcc ccttcatcga gaacgatgcg
     2401 gtgctgggcg atgtcgacga gcatttcggc ggcgattatc tgaatgaaga aaccatcctg
     2461 aagatggcgc gcaccaaggg ttttagtacg gcggcgatcg gcaaggtcgg tccgacgctg
     2521 attttcgacc acaccgaccg cgccgataag cccggcctgc attcgatcgt gatcgacgat
     2581 tccaccggcg gcaagaacgg cgtgccgctg tcggaggaga tgaaggccgc gttgacaaaa
     2641 gccaatcttc cgctcgcggc accgtcccgc ggcgacaacg gcaaggccgg cgacgccaag
     2701 acccctggca ccgtggtgcc gaataccgcg cagcaggcct atttcgccga cgtcgccagc
     2761 agaatcgtgc tgccactgtt caaggcgcgc gcaaaaccgt tcgtgctggt gttctggtcg
     2821 cgcgatcccg acggcaccca gcacaatacc ggcgacagcc tcaacaccat cacgcccggc
     2881 atcaacgggc cgacctcgct cgccggcatc aagaacgccg acaacaatct ggcgcaattg
     2941 cgcgaggcgc tcgacgagct cggcctctcc gccaccacca acgtcatggt cgccgccgac
     3001 cacggcttct cgaccatttc gaaggagagc aagaccagcc cctcggccaa agcgacctat
     3061 gaggacacgc cgaaggattt cctccccatg ggttttctcg cgatcgatct tgcgaaagcg
     3121 cttgacctgc cgctgttcga tccgaacgac aagaacgcgc gtattgcgga aaatgctcac
     3181 cccaaggccg gcaacggtgt gctcgggcag gatcccgcaa aacccgatct ggtggtcgcc
     3241 accaatggcg gatccgacct gatctatttg ccgaaaaagg acaagaagct ggcgggacgc
     3301 gcggtgaagg cgctgctgga acaggattac gtgagcggca ttttcgtgga cgacgcgctt
     3361 gggcgttatc ccggcgcgct gccgctttcc gcgctcaacc tgaaaggcaa ggcggtcacg
     3421 ccgcagccgt cgatcgtggt caatttccgc tcctacgcca gcggttgcga cgagccgacc
     3481 acctgctcgg tggaggtggc ggacaccgtg ctgcggcagg gacagggcat gcacggcggt
     3541 ttcagccgcg gcgacaccat gaatttcatg gcggcggtag ggcccgactt caaggctggc
     3601 tatgtcgatg cgttgccggt gagcaacgcc gatgtcggca tgaccattgc gcagcttctg
     3661 gatttgcatg cggcagccgt cggcggactg accggccggg tgatgtcgga agccttgcca
     3721 aacggaatcg tgtcaaagtt tgccgacggc acgatcagct caaagcccga tccgaacggc
     3781 ttgcagacgg tcgtgaaatt ccagcgtgtc ctctcgcagc gctattttga cgtcgcggga
     3841 ttccccgggc ggacccttgg gcttgaggcc ggcaagcaaa aaacggcggg gaagtaaccc
     3901 ccgccgctgc gatacccacc ccaacgcgct gaatttacat cccgatgtcg aacaccttcg
     3961 gcaactgcga gaacggcagc gccgccgcgc cgacgatggt ggcgaccatt cgcatcaagg
     4021 tgcgattgct gcggcgtttg ccgcgtatct ggcttgcgat ccaatccagc acctcgctat
     4081 tgacgccggc agccctggca ggcgcccagc tttcgatcgc ggtatccggc gacagcgcaa
     4141 tggtgcgcga cggaaccggc agaccggact cggaggccgc gtggtgcacg acggccttgg
     4201 ccagcaaatc gtcgaaacgt cc
//
LOCUS       DASWGM010000656        10061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_190534,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000656 DASWGM010000000
VERSION     DASWGM010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190534"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..652)
                     /locus_tag="VGA15_18410"
     CDS             complement(2..652)
                     /locus_tag="VGA15_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX9589704.1"
                     /translation="MDPEGKKPRIGRRKAAGMGDNSAGRGYLDGRLLIAMPVMGDPRF
                     ERSVIYLCAHSSEGAMGIIVNRPAGSIDFPELLVQLDIIKKADQIKLPENAESMKVLK
                     GGPVDTGRGFVLHSSDFFIKDATLRIDDGICLTATVDILKAIARGAGPKHAILALGYA
                     GWAPGQLENEIQDNGWLHCDADPDLVFGSDVEEKYLRALRKIGIDPGMLSNEAGHA"
     gene            789..1625
                     /locus_tag="VGA15_18415"
     CDS             789..1625
                     /locus_tag="VGA15_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX9589705.1"
                     /translation="MIMVPMRAALGLAAAIVASSLAIEARAEDASPWQRDGHSAVRLL
                     AGSRSGAVLLGGIAVQLQPGWKTYWRTPGDSGVPPRFDFSKSENIEAVTVLWPAPAKF
                     DDGAGGHSMGYHDQVVLPLRIVAKNTDKPVMLRVGINYAVCEKICIPVEANTELGFTS
                     VASTEDSALFAALDTVPKPASIGDPNPLTIRDVKRDGKSSVLVDVVTPDTREINLFVE
                     GPTPDWALPVPTLLEKSPPGVKRFSFELDGLPPGADPAGAALKLTLVGGDRAYEFNIN
                     LE"
     gene            1825..3195
                     /locus_tag="VGA15_18420"
     CDS             1825..3195
                     /locus_tag="VGA15_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX9589706.1"
                     /translation="MAVIDAPSTTALPAGVIARLRSMLGGSGEASVTTRLAGTIFIIR
                     VISAALAYLSQILLARWIGGSDYGVYVYVWTWVLLLGSMMDFGISASAQKIIPEYRTS
                     GEHALLRGFVSGSRWMTFVVSALVSVLLAGVVKGLSPWIDANAALPLYIGCVTLPAFV
                     VANTQDGIARSHDWMRLGLMPQFIVRQSLIIGFTAGAFVLGFDLGATAAMLASAAAVW
                     IAMIGQMMVLNRRLAGHIGPGPKAYDFRGWLAISLPILMVEGFYLLLSYTDVLVLQQF
                     RSSQEVGVYFAVVKTLALVSFIHYAMSATTAHRFAEYHALGDRARLSAYVAHAIKWTF
                     WPSLAATILLVALGKPLLWLFGPQFITGYDIMFIAAIGLVVRSAIGPVERLLNMLGHQ
                     HICALAYALAFVMNGVLCVALVPRFGGHGAAAATSISLVFETVLLFWIVRRRLGLHVL
                     AFGSSD"
     gene            complement(3354..4142)
                     /locus_tag="VGA15_18425"
     CDS             complement(3354..4142)
                     /locus_tag="VGA15_18425"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX9589707.1"
                     /translation="MATLTGRTAVVTGSTSGIGLAYARAFAGAGANIVLNGMGAPADI
                     EKERSAIESDFKVKAVHSPADMTKPAEIAGMIALGETTFGSVDVLVNNAGIQFVSPIE
                     EFPPEKWEAIIAINLSSAFYGIRAAVPGMKKRGWGRIINTASAHSLVASPFKSAYVSA
                     KHGIAGLTKTVALELATFKITCNCISPGYVWTPLVEHQIPETMKARSMTKEQVIHDVL
                     LAAQPTKEFVTSEQVAALALFLCGDDAAQITGANLSIDGGWTAE"
     gene            complement(4148..5323)
                     /locus_tag="VGA15_18430"
     CDS             complement(4148..5323)
                     /locus_tag="VGA15_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
                     /protein_id="HEX9589708.1"
                     /translation="MDISDPTPATTPANAMRVLVLQGGGALGSYQAGAYQALCHHDFE
                     PEWIAGISIGAINAAIIAGNPREKRIDRLKEFWEMVSSPVPWNPVTKSDRGRSLFNET
                     SAALIATFGVPGFFTPRIPPAPLWPQGTPQSQSYYDTAPLKKTLERLVDFDMINDLKT
                     RLSVGAVSVTTGNFKYFDNFEFRKLGKKIGPEHIMASGALPPGFPSIVIDGEHFWDGG
                     IATNTPLDFVLDAEITRDLLIFQVDLFSARGPLPGSLLEAAEREKDIRFSSRTRMNTD
                     KNRQIHNARKALRELIGKLPDYLKNDPSVEILRDAAKESTVTVVHLIYRSKNYESSSK
                     DYDFSHVGMVEHWGAGVRDVHLSMRHEDWLERPQSGETMMTYDLTGDGTETLDGKKE"
     gene            complement(5421..5924)
                     /locus_tag="VGA15_18435"
     CDS             complement(5421..5924)
                     /locus_tag="VGA15_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX9589709.1"
                     /translation="MRAAVAIIGFLALGGCAGEAPLPDQPTMYLNMANSGAKLDSQAA
                     ASMISLYRENNGLGGVAVDPELMKLAESQSQAMAGRNKPDHDVKAPLAKRLNASGYPA
                     TLAVENVSAGYHTLAEAFSGWRDSPPHRANMLKNGVTKLGIAASYAPNTKYKVFWTLI
                     LASSDPR"
     gene            complement(6333..7058)
                     /locus_tag="VGA15_18440"
     CDS             complement(6333..7058)
                     /locus_tag="VGA15_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX9589710.1"
                     /translation="MTHDIERRDFLHGVGVAAGIAAASTLIQKPAHAQAAAPSGAKPM
                     AYEIKPFSLDPKSVKGISEKILISHYENNYVGAVKRLTAIAAQLAELDFSKAPGFVIN
                     GLKREELIAMNSMILHEAYFDSLGGGGAARGSLADAITRDFGSVERWRAEFSAMGKAE
                     GGGSGWVLLMYSPRDKRLVNQWAADHTNAVAGGRPIIALDMYEHAYHMDYGAKAGGYV
                     DAFMEAINWDNAVKLYERHSRDG"
     gene            complement(7433..8482)
                     /locus_tag="VGA15_18445"
     CDS             complement(7433..8482)
                     /locus_tag="VGA15_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEX9589711.1"
                     /translation="MAIEVKNIVKKFGAFAALDGVDLKIANGELLALLGPSGSGKTTL
                     LRIIAGLDWPDSGEVSFDGEDALARGAGERHVGFVFQHYALFRHMTVFENVAFGLRVQ
                     PRAVRKKEASIRARVRELLDLVQLDWLADRYPSQLSGGQRQRIALARALAIEPRILLL
                     DEPFGALDAKVRKELRQWLRSLHNEINVTSIFVTHDQEEALEVANRVVVMDKGRIEQI
                     GTPGEVYDNPATAFVHGFIGESIVLPVNVSDGCVRLGGKPLNIEVNGAASGASKLFVR
                     RHDMQVGPAGSGALEGAVRHVRSFGPIQRAEIALCAGECATVIEIDAPRDRELQCGDI
                     VGLQPRRYRIFAAQD"
     gene            complement(8472..9341)
                     /gene="cysW"
                     /locus_tag="VGA15_18450"
     CDS             complement(8472..9341)
                     /gene="cysW"
                     /locus_tag="VGA15_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX9589712.1"
                     /translation="MNAALPSSITARDDLRTEPRLIRGVIIAIAVIFLTVFVVLPLVV
                     VFAQAFSKGVGAYFAALSDPEALSAIRLTLMVAVISVGLNLVFGLVAAWAIAKFDFRG
                     KTFLISLIDLPFSVSPVISGLVFVLLFGAQGFWGPWLQSHDVHVLFAVPGIALATIFV
                     TFPFVARALIPLMQEQGTQEEEAAISLGASGLQTFFRVTLPNIKWGVLYGVLLCNARA
                     MGEFGAVSVVSGHIRGETNTMPLLVEILYNEYQFISSFAIASLLAMLALITLVAKTIL
                     ERHLDEGPSPSGD"
     gene            complement(9588..>10061)
                     /locus_tag="VGA15_18455"
     CDS             complement(9588..>10061)
                     /locus_tag="VGA15_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9589713.1"
                     /translation="QTGWLGAPLAELGIKVAFTPVGIFVAMIFIGIPFVVRTVQPVLI
                     DLDTEIEEAAASLGANRWHTVSKVILPSLIPALLTGFALAFARAVGEYGSVIFIAGNL
                     PNVSEIAPLLVVIRLSEFRYADATAIAVVMLVASFLIIFAVNRIQRWAQTRIPAH"
BASE COUNT         1881 a         3146 c         3055 g         1979 t
ORIGIN      
        1 gctacgcatg cccggcttcg ttcgacagca tgccgggatc gatcccgatc ttgcgcagcg
       61 cgcgcaggta tttttcctcg acgtcgctac cgaacaccag atccggatcg gcgtcgcaat
      121 gcagccagcc attgtcctgg atttcgttct ccagttgtcc cggcgcccag ccggcataac
      181 ccagtgcgag gatcgcgtgc ttcggcccgg cgcccctggc gatcgccttc agaatatcga
      241 ccgtcgcggt cagacagatt ccgtcatcga ttctcagggt cgcatccttg atgaagaaat
      301 cgctcgaatg cagcacgaag ccgcggccgg tatcgaccgg gccgcccttc agcaccttca
      361 tggattcggc attttccggc agcttgatct ggtcggcctt cttgatgatg tcgagctgca
      421 ccagcaattc cggaaagtcg atgctgccgg ccgggcgatt gacaatgatg cccatggcgc
      481 cttcggatga atgcgcgcag agatagatca ccgagcgctc gaagcgcgga tcccccatta
      541 ccggcatcgc gatcagcaac cggccgtcga gataaccgcg ccccgccgaa ttgtcgccca
      601 tgccggcggc tttgcggcga ccaatccttg gttttttacc ttcagggtcc atccgcaaag
      661 gactctcacg ttgatgcaca tcctgatatc gggtgccggt tctgtcaatc aagctttgcg
      721 ccggcgttcc ttaccgcatc acaagcaatt caatggcttg caaccttgct tggctgtaat
      781 gacgtgtcat gatcatggtt cccatgcgcg ccgcgctcgg cctggccgca gccatagttg
      841 cttcgtccct ggcaatcgag gctcgcgccg aggatgcgtc gccctggcag cgggatgggc
      901 actccgcggt gcggctgctg gcggggtcgc gcagcggcgc cgtgctgctc ggcggaatcg
      961 ccgtccagct tcagccggga tggaaaacct actggcgcac gcccggcgat tccggcgtgc
     1021 cgccgcgctt cgacttctcc aaatcggaaa atatcgaagc cgtcaccgtg ctgtggccgg
     1081 cgcctgcgaa attcgacgat ggcgcaggcg gccactccat gggttaccac gaccaggtcg
     1141 tgctgccgtt gcgaattgtc gcgaaaaata ccgacaaacc ggtgatgctt cgcgtcggga
     1201 tcaattacgc ggtctgcgag aaaatctgca ttcccgttga ggccaatacc gagctcggct
     1261 tcaccagcgt ggcgagcacc gaggacagcg cgctgttcgc cgccctcgac acggtgccga
     1321 agcccgccag catcggcgat cccaacccgc tgaccatccg cgacgtcaag cgtgacggca
     1381 aatcctccgt tctggtcgat gtcgtcacgc ccgacaccag ggaaatcaac ctgtttgtcg
     1441 aaggcccgac gccggactgg gcgctgccgg ttccgacgct gctcgaaaag agcccgcctg
     1501 gggtcaagcg cttctcattc gagctcgacg ggctgcctcc cggcgcggac cccgcaggcg
     1561 cggcgctcaa attgaccctg gtcggcggcg accgcgctta cgaattcaat atcaatctgg
     1621 agtagccccg cgtcaccccg gcgaacgccg ggacccatac gccgaggact cgcttttcgg
     1681 caacgtggcc gacgccctcc gtacaataaa cgccaggggc tatggatccc ggccttcgcc
     1741 gggacgacac gagagaccga atcttaacga atggttgata ggccggatga ttgccgcacc
     1801 atgcggtgcc gaaggatttc cgctgtggcc gtgatcgacg caccatccac aacagctttg
     1861 cccgcaggcg tgatcgcacg gctgcgctcg atgttgggcg gttccggcga agcgtccgtt
     1921 accacgcgtc ttgccggcac gatcttcatc atccgcgtta tcagcgcggc gctggcctat
     1981 ctttcgcaga tcctgctcgc gcgctggatc ggcggttcgg attacggcgt ctatgtctat
     2041 gtctggacct gggtgctgct gctcggcagc atgatggact ttggtatttc ggcgtcggcg
     2101 caaaaaatca ttccggaata tcgcaccagc ggcgaacacg cgctgctgcg aggttttgtc
     2161 tccggcagcc gctggatgac ctttgtcgtt tctgccctgg tttcagtgct gctcgcaggc
     2221 gtcgtgaaag ggctgtcgcc atggatcgac gcgaacgcgg cgctaccgct ctacatcggc
     2281 tgcgtcacgc tgccggcctt cgtggttgcc aacacccagg acggcatcgc acgttcgcac
     2341 gactggatgc ggctcggatt gatgccgcaa ttcatcgtgc ggcagtcgct gatcatcggc
     2401 ttcaccgccg gcgccttcgt gctcggcttt gatcttggcg caaccgccgc gatgctcgcc
     2461 agcgcggccg cggtgtggat cgccatgatc gggcagatga tggtgctgaa ccgcaggctc
     2521 gccggccaca tcgggcccgg cccaaaggct tacgatttcc gcggctggct ggcgatctcg
     2581 ctgccgatcc tgatggtgga aggcttttac ctgctgctgt cctacaccga cgtgctggtg
     2641 ctgcagcaat ttcgctcttc gcaagaggtc ggcgtctatt tcgccgtggt gaagacactg
     2701 gcgctggttt cgttcattca ctacgcgatg tcggcgacaa cggcgcatcg tttcgccgag
     2761 tatcacgccc tcggcgacag agcgcggctg tcggcctatg tcgcacacgc gatcaaatgg
     2821 acgttctggc cgtcgctggc cgcgaccatc ctgctggtgg ctttgggcaa gccgctattg
     2881 tggctgttcg gtccgcaatt catcaccggc tacgacatca tgttcatcgc tgccattggc
     2941 ctcgtggtgc gctctgcgat cggcccggtc gagcggctgc tcaacatgct cggtcaccag
     3001 catatctgtg cgctggccta cgcgctggcg ttcgtcatga atggggtgct ttgcgtggcg
     3061 ctggtgccgc gcttcggcgg ccacggtgcc gccgcggcga cctcgatctc gcttgtgttc
     3121 gaaaccgtgc tgctgttctg gattgtgcgg aggcggctgg gcctgcacgt gctggcgttc
     3181 gggagcagcg actgaatccc tccacgtcat gcccggactt gatccgggca tccatcatcc
     3241 tcgcaaggct ttttcagttt gatggattgc cgggtcatct agtgcgaaga cgcgcttcgc
     3301 gcttttgccc ggcaatgacg aatcgtgaag ttgagattgc agaggatcct cccctactcc
     3361 gcggtccagc cgccgtcgat cgagaggttg gcgccggtga tttgggcggc atcgtcgccg
     3421 cagagaaaca gcgccagggc tgcgacctgc tcggaggtga cgaactcctt ggtcggctgc
     3481 gccgccagca gcacgtcgtg gatgacctgc tccttggtca tgctacgcgc cttcatggtc
     3541 tctggaatct ggtgctcgac cagcggcgtc cagacataac ccgggctgat gcagttgcag
     3601 gtgatcttga aggtcgcaag ctccagcgcc accgtcttgg tgagaccggc gatgccgtgc
     3661 ttggccgaca cataggccga cttgaacggc gaggccacca gcgaatgcgc ggaagctgtg
     3721 ttgatgatgc ggccccagcc gcgtttcttc atgccgggta cggcagcgcg gataccataa
     3781 aacgccgacg acaggttgat cgcgatgatc gcttcccatt tctcgggcgg aaactcctcg
     3841 atcggcgaca cgaactggat gccggcattg ttgacgagaa cgtcgaccga gccgaacgtt
     3901 gtttcgccca gcgcgatcat gccggcgatt tccgcgggct tggtcatgtc ggcgggcgaa
     3961 tgaaccgcct tgaccttgaa gtcgctctcg atcgcggaac gttccttttc gatgtcggcg
     4021 ggcgcgccca tgccgttgag aacgatattg gcgccggcgc cggcaaaggc gcgcgcatat
     4081 gccagtccga tgccgctggt cgagccggtc acaaccgcgg tcctgcctgt caacgtagcc
     4141 atttcatcta ctcctttttg ccgtcgaggg tttcggtgcc gtcccccgtg agatcgtagg
     4201 tcatcatggt ttcgccggac tgcggccgct ccagccaatc ttcgtgacgc atcgacagat
     4261 gaacgtcgcg cacgcccgcg ccccagtgct cgaccatgcc gacatgcgag aaatcataat
     4321 ccttggagga ggattcgtag ttcttgctgc ggtagatcag gtgcaccacc gtcaccgtgc
     4381 tttccttggc ggcgtcgcgc aaaatctcga ccgacggatc gttcttgaga taatcgggca
     4441 acttcccgat caattcgcgc agcgccttgc gggcgttgtg aatctgccgg ttcttgtcgg
     4501 tgttcatgcg ggtgcggctg gaaaagcgga tgtccttctc gcgctcggcg gcctcaagca
     4561 gcgaacccgg cagcggcccg cgcgcgctga acaggtcgac ctggaagatc aggaggtcgc
     4621 gggtaatttc cgcatccagc acaaaatcga gcggcgtgtt ggtggcgatg ccgccgtccc
     4681 agaagtgttc gccatcgatg acgatggacg ggaagcccgg cggcagcgcg ccggacgcca
     4741 tgatatgttc cgggccgatc ttcttgccga gcttcctgaa ttcgaaattg tcgaaatatt
     4801 tgaaattgcc ggtcgtgaca ctcacggcgc cgacgctcag ccgggtcttc agatcgttga
     4861 tcatgtcgaa atcgaccaga cgttccagtg tcttcttgag cggcgcggta tcgtaataac
     4921 tctgcgactg cggcgtgccc tgcggccaaa gcggcgccgg gggaattcgc ggcgtgaaaa
     4981 agccgggaac gccaaaggtg gcaatcaggg cggcgctggt ttcgttaaaa agcgagcgcc
     5041 cgcggtcgct cttggtaacg ggattccacg gcaccggcga agaaaccatc tcccagaatt
     5101 ccttcaggcg gtcgatccgc ttttcgcgcg ggttgccggc gatgatcgcg gcgttgatgg
     5161 cgccgatcga aatgccggca atccattccg gctcgaaatc gtggtggcac agcgcctggt
     5221 aggcgccggc ctggtaggag ccgagcgcgc cgccgccttg cagcacaagc acgcgcatcg
     5281 cgttggcggg cgtcgtagcg ggcgttgggt cggaaatatc catcatgaca tcggatcaaa
     5341 tgcgaaaagg aggattccac cgtgaggcca gttcgcgatg gcgtcaatca gcgagcctcc
     5401 cgagtgggat cacgccgagt ttatcgcggg tccgacgatg caagaatcag cgtccagaac
     5461 accttgtatt tggtattcgg agcatagctc gccgcgatgc ctaattttgt gacaccgttt
     5521 ttcagcatgt tggcccggtg cgggggcgag tcgcgccagc ccgaaaacgc ttcggccagc
     5581 gtatgatatc cggcggacac gttttccacc gccagtgtcg cggggtatcc ggacgcgttc
     5641 aggcgcttgg ccagcggggc tttgacgtca tggtcgggct tgttgcggcc tgccatcgcc
     5701 tgcgactgcg attccgcaag cttcatcagt tccggatcga ccgccacgcc gccaaggccg
     5761 ttgttttccc ggtacagcga gatcatcgag gccgcggcct gggaatcgag cttggcgccg
     5821 ctattggcca tgttgagata catggtgggc tggtcgggca gcggagcctc ccctgcgcag
     5881 ccgcccagcg ccaaaaaccc gataatcgcg accgccgcgc gcatgattcg aaattcccgc
     5941 gcgcatgatc cgccaaagtg tgagcgggtt ggcgataaga tcattcgctc tttcaatagt
     6001 ttagagcgcg atcggaggca aaaccggata ccgcttttgc tgatcgcgct gtgatgaaag
     6061 ccggttgttg cataccaaca gtggctgaat ggtgaacgat gggggttaat tcgagtgtca
     6121 ttccggggcg aggcgaagcc tcgaacccgg aatccagaga tgatcggagc gagattccgg
     6181 gttcgcgctt acgcgcgccc cggaatgacg agctatattg cgtggcaaaa atccgtctag
     6241 cggccgggcc gcggccggac aatctcgccg ctcccaagtt cccgatcacg cggcgcgtcg
     6301 atcccgatcc ggaaaacccg ctttcgccgg gctcagccgt cccgactgtg gcgttcgtag
     6361 agcttgacgg cattgtccca gttgatcgct tccatgaacg catcgacgta gccacccgcc
     6421 ttggcgccat agtccatgtg ataggcatgc tcgtacatgt cgagggcgat aatcggccgt
     6481 ccgcccgcaa ccgcattggt atggtcagca gcccattgat tgacgagacg tttgtcgcgg
     6541 ggcgaataca tcaacagcac ccagccggaa ccgccgccct ccgccttgcc catcgccgaa
     6601 aattccgcgc gccaacgttc gacgctgcca aaatcgcgcg tgatcgcgtc cgcaagagag
     6661 ccgcgcgccg ctccaccgcc gccgaggctg tcgaaatagg cctcgtgcag gatcatcgag
     6721 ttcatcgcga tcagctcttc gcgcttcaac ccgttgatga cgaaccccgg cgccttggaa
     6781 aaatccagtt cggcgagttg tgcggcaatc gcggtcaagc gcttgaccgc gccgacgtag
     6841 ttgttttcgt agtggctgat aaggatcttt tcggagattc cctttaccga tttcggatcg
     6901 agcgaaaacg gcttgatctc atacgccatc ggttttgctc ccgatggcgc cgccgcctgg
     6961 gcgtgcgccg gcttttgaat cagcgtcgac gctgccgcta tccccgcggc cactcctacg
     7021 ccgtgcagaa aatcccgacg ttcgatatcg tgtgtcatcg ctcacgctcc tggtttttac
     7081 tgatcgtcga tcaattcggt cccatcgaaa tcgctcgcga aaggaacggc gccacgctgg
     7141 tgccatcgac acgaacatcg atgagcgcct ggcgcatggg cgcgccaatc aggcgagcgg
     7201 atttatcgga tgagattggg tcgacggcag acatgtcgcg tccttgtgga acaatcggac
     7261 gcgattcttt ggcatgtcgc ctcatgagga gatcgcttcc ccatgcgtca aatacactcg
     7321 acgaagagcc ggcttgtcaa tcggatttcc tctgtccgat cacgacctct cgctatgttg
     7381 aaggcgccaa caaatgtgac cggaaggtga taggcaacaa ggcgttttca gttcagtcct
     7441 gcgcggcgaa aatccggtag cggcggggtt gcagcccgac aatatcgccg cactgaagtt
     7501 cccgatcccg cggcgcgtcg atctcgatca cggtcgcgca ctcgcccgcg cacaaggcga
     7561 tttccgcacg ctggatcggg ccgaacgacc ggacatgccg caccgcgccc tccagcgcac
     7621 cgctgccggc cggcccgacc tgcatgtcat ggcggcggac gaacagtttc gatgcgccgg
     7681 aagccgcacc attcacctcg atattcagcg gcttgccgcc gagccgcacg cagccgtcgc
     7741 tgacattgac cggcagcacg atcgactcgc cgatgaagcc gtgcacgaac gcggttgcgg
     7801 gattgtcata gacctcgccg ggcgtgccga tctgctcgat gcggcccttg tccatcacca
     7861 ccacccggtt ggcgacctcg agcgcctctt cctgatcgtg ggtgacgaag atcgaggtca
     7921 cgttgatttc gttatgcagc gagcgcagcc attgccgcag ttccttgcgc accttggcat
     7981 cgagcgcgcc gaacggctcg tcgagcagca aaatccgcgg ctcgatcgcc agcgctcgcg
     8041 caagcgcaat gcgctggcgc tgaccaccgg acaattggct cgggtaacga tcggcaagcc
     8101 agtcgagctg caccagatcc agcagttccc tgacccgcgc gcggatgctg gcctctttct
     8161 tgcgaaccgc acggggctgc acccgcaggc cgaacgcgac gttctcgaac accgtcatgt
     8221 ggcggaacag cgcatagtgc tggaatacga atccgacatg gcgctcgccg gcgccgcggg
     8281 caagcgcgtc ctcaccgtcg aacgaaacct cgccggaatc aggccagtcg agcccggcga
     8341 tgatccgcaa cagcgtggtc ttgcccgagc ccgacggccc cagcagcgcc agcagttcgc
     8401 cgttggcgat tttgaggtcg acaccgtcga gcgcggcaaa cgcgccgaat ttcttcacga
     8461 tatttttgac ttcaatcgcc actcggtgat ggtccttcgt ccagatgccg ctcgagaatg
     8521 gttttcgcca ccagcgtgat caaagccagc atcgccagca gcgatgcgat cgcgaacgaa
     8581 gatatgaatt ggtattcgtt gtagagaatt tcaaccagca gcggcatggt gttggtctcg
     8641 ccgcgaatgt ggccggatac caccgagacc gcgccgaact cacccattgc ccgcgcattg
     8701 cacagcagca ctccgtacag cacaccccat ttgatattcg gcagggtgac gcggaagaag
     8761 gtctgcaggc ccgacgcgcc gagcgagatc gcggcctctt cctcctgcgt gccctgttcc
     8821 tgcatcagcg gaatcagcgc gcgcgcgacg aacggaaacg tcacgaaaat ggtcgccagc
     8881 gcaatgccgg gcaccgcgaa cagcacatgg acgtcatggc tttgcagcca tggcccccaa
     8941 aaaccctgcg cgccgaacag cagcacgaag acgaggcccg aaatcaccgg gctgaccgag
     9001 aacggcagat cgatcagcga gatcaggaaa gtcttgccgc gaaagtcgaa tttggcgatc
     9061 gcccaggccg cgaccagccc gaacaccaga ttgaggccga ccgaaatcac cgcgaccatc
     9121 agcgtcagcc tgatcgccga cagcgcttcc ggatcggaca gtgcggcgaa ataggcacca
     9181 acccccttgg aaaaggcctg cgcgaacact accacaagcg gcagcacgac gaagacggtg
     9241 aggaagatca cggcaatcgc gatgatgacg ccgcgaatca acctgggctc ggtgcgcaga
     9301 tcgtcccgtg ccgtgattga tgagggtaga gctgcgttca tcgcgaacgt ttcaaggacg
     9361 gcagcgcgct ccctctccca tccaactcgg gtttacccga gttggaaatc tcaaatgtcg
     9421 aagtcggata tatccgactt cgattgggag aggtttgggg tgagggggta cggtctatcg
     9481 ttattctgta acccctcacc cggatcgcca aggcgatccg acctctcccg atgggagagg
     9541 tgaaccagag tcgctgccgc ggcattttcc aaatcatgca tcaatctcta atgcgcgggg
     9601 attcgggttt gcgcccagcg ctggatccga ttgacggcga agatgatcag gaatgacgcg
     9661 accagcatca ccacggcgat cgcggtggca tcggcatagc ggaattccga gagccggatc
     9721 acgaccagca gcggtgcgat ctccgaaaca ttcggcagat tgccggcgat gaagatgacg
     9781 gatccgtact caccgacggc acgcgcgaac gccagcgcaa atccggtcag cagcgccggg
     9841 atcaggctcg gcaggatcac cttggagacc gtgtgccaac ggttggcacc gaggctggcg
     9901 gcggcttcct cgatttcggt gtcgaggtcg atcagcaccg gctgcaccgt ccgcaccaca
     9961 aagggaatgc cgatgaaaat catggcgacg aaaattccga ccggcgtgaa cgccaccttg
    10021 ataccgagtt cggcaagcgg cgcgccgagc cagcctgttt g
//
LOCUS       DASWGM010000657         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2579553,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000657 DASWGM010000000
VERSION     DASWGM010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2579553"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /gene="tssI"
                     /locus_tag="VGA15_18460"
     CDS             complement(<1..607)
                     /gene="tssI"
                     /locus_tag="VGA15_18460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEX9589714.1"
                     /translation="MAQLTQDKRLAQLQTPLGKDVLVLASFVATEGLSELFEVNVETL
                     SEKENINFDDAIGRACTIKQITYDNKTRYYNGILTATRRTGSTADLFHYHLTLRPWLW
                     LLSRKADCRIFLKKDVKEIISEVFKKAGFTDFVFRTTGDFHKIPYCVQYRETDLAFCS
                     RMMEHYGIYYFFEHADGKHTLVMADARTSHKSIPDLPKLPFI"
     gene            complement(1135..2016)
                     /locus_tag="VGA15_18465"
     CDS             complement(1135..2016)
                     /locus_tag="VGA15_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX9589715.1"
                     /translation="MSSLASKQGPRYRHTADETAPYEIIGVEKLTPIIGAEISGVDLA
                     GALSNRTMDEIHRALAENLVIFFRDQPITPEQHLAFGRQFGELHIHPAAPHEGDDPAL
                     MKIYADKDSPRANGEGWHSDVSCDVEPPMGSILHIRQCPPRGGDTLFASMYAAYESLS
                     DRMKTYLDGLTALHDGEPIYRGLYANYGVADRPAYPHAEHPVVRTHPVTGKKALYVNR
                     GFTRHIVGIPGDESHAILNYLYQHAENPLFQCRFRWTENAIAFWDNRCAQHRAMWDYW
                     PHTRAGTRVTVKGERPV"
     gene            2137..2325
                     /locus_tag="VGA15_18470"
     CDS             2137..2325
                     /locus_tag="VGA15_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589716.1"
                     /translation="MDKYGRSGEPSLLPQAKEGERHPIFGALKGLIWVPPGIDLTEPA
                     DPDWGKVHETNATIPARR"
     gene            complement(2407..3699)
                     /gene="ispG"
                     /locus_tag="VGA15_18475"
                     /gene_synonym="gcpE"
     CDS             complement(2407..3699)
                     /gene="ispG"
                     /locus_tag="VGA15_18475"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX9589717.1"
                     /translation="MNQPVKPPQDDVAGPNARRKTTQVMVGNVAVGGGAPIVVQSMTN
                     TDTADVAGTVAQIAALSRAGSELVRITVDRDEAAAAVPHIRDELRKRGITTPLIGDFH
                     YIGHKLLADYPACAEALDKYRINPGNVGFKNKRDSQFADIVEIALKNNKAVRIGANWG
                     SLDQELLTRLMDENALLAEPKDVRAVTREAMVQSALLSAARAEEIGLPKNKMILSAKV
                     SAVQDLIAVYQTLASRSGYAIHLGLTEAGMGSKGIVASAAALGILLQDGIGDTIRISL
                     TPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTTFQELARSIQDFIRD
                     EMPSWKTRYPGVESLNVAIMGCIVNGPGESKHADIGISLPGTGETPAAPVFVDGKKFR
                     TLRGPNIATEFKAMVIDYIDQRYGAGASAPAGTVTAAE"
BASE COUNT          728 a         1104 c         1199 g          781 t
ORIGIN      
        1 ggatgaacgg gagcttcggc agatccggga tcgacttgtg ggacgtccgc gcgtccgcca
       61 tcaccagcgt gtgcttgccg tcggcgtgct cgaaaaagta ataaataccg tagtgctcca
      121 tcatgcgact gcagaacgcc agatcggttt cgcgatactg gacgcagtag ggtatcttgt
      181 gaaaatcacc ggtggtgcgg aatacaaagt cggtaaatcc cgcctttttg aacacctccg
      241 aaatgatttc cttgacgtcc ttcttgagaa aaatacgaca atcggccttt cggctgagca
      301 gccacaacca cgggcgcagc gtgagatggt aatgaaagag gtctgccgtg ctgccggtcc
      361 gtcgtgtcgc cgtcaggata ccattgtagt agcgggtctt attgtcatag gtaatctgct
      421 tgatcgtgca cgcccggccg attgcgtcgt cgaaatttat gttttccttt tcgctgagcg
      481 tttcgacgtt gacttcgaat aattcactta atccctccgt ggcgacgaaa cttgccaaga
      541 caagaacatc cttgccgagc ggagtctgca attgggcgag acgcttatcc tgagtaagct
      601 gagccatcag cgatttccct tcgaggctgc atgcgagtcc gccatgtcga ccagctcgtg
      661 gctgtttctc gaggccaggc aatgaaaggg atgcgacatt tacagtatcc tatcgaatcg
      721 cggcctcctc acccactcga tgcgcgggcc gcatcccctg atcagaccgc tgaacagttc
      781 caattaggac gcagcacagg ccgttatggt tcattttctg aataggaaaa tgttctgaat
      841 aggaaaatgc atcttcggaa atcattccag tgcttcaaac gacaaagacg cggctttttg
      901 cgacaactca tcaactattt tgacgcgaag gagcaggata cacggaaaag ctgaaagagg
      961 tcaggacgca tcggccacgc ccaacgtcca tctggctgga gagggcgtga agtccgcttc
     1021 ggggtcattc gctaccgggt cgagccaaag ccaggtccgg ccatgtccgc tatgccgacg
     1081 aaagcggaag taaattcagc gcagtagcgg ccccgctctc aggacggaga tccgtcacac
     1141 cggccgttcg cccttcaccg tcacccgcgt gccggcgcgg gtgtgcggcc agtagtccca
     1201 catcgcgcgg tgctgggcgc agcgattgtc ccagaacgcg atggcgtttt cggtccagcg
     1261 gaagcggcac tggaacagcg gattttccgc gtgctggtag agatagttca atatcgcgtg
     1321 gctctcgtcg ccggggatgc cgacgatgtg gcgggtaaaa ccgcgattga cgtagagcgc
     1381 cttcttgccc gtcaccggat gggtgcgcac caccggatgc tcggcgtgcg gataggccgg
     1441 ccggtcggcg acgccgtaat tggcgtagag cccgcgatag atcggctcgc cgtcgtgcag
     1501 cgccgtcagc ccgtcgagat aggtcttcat ccggtccgac agcgattcat aggcggcata
     1561 catgctggcg aacagcgtgt cgccgccgcg cggcgggcat tgccggatat ggaggatcga
     1621 gcccatcggc ggctcaacgt cgcaggagac gtccgaatgc cagccctcgc cgttggcgcg
     1681 cggcgaatcc ttgtcggcat agatcttcat cagcgccgga tcgtcgccct catggggagc
     1741 ggcgggatgg atgtgcagct cgccgaactg gcgcccaaag gcgagatgct gttctggggt
     1801 gatgggctga tcgcgaaaga agatcacgag attttcggcg agcgcgcggt ggatctcgtc
     1861 catggtccgg ttggagagcg cgccggcaag atcgacgccc gagatttccg caccgatgat
     1921 aggggtgagt ttttcgaccc cgatgatctc gtagggcgcc gtttcgtcgg cggtgtgccg
     1981 atagcgggga ccctgcttgc tggcgagcga agacatggtt cctccggtga gtttcttatg
     2041 ggttatcgta gccagggcac agcgaagcgc aatccaagcg ggcacgatat ttggtacact
     2101 tggagggctt agtctgacga acagatggag gcgggaatgg acaaatacgg ccgatcgggc
     2161 gaaccttcgc tgcttccgca agcaaaagaa ggcgaacggc atccgatatt cggggcattg
     2221 aaggggctca tttgggttcc cccgggcatc gatctaacgg agccggccga tcccgactgg
     2281 ggcaaagtcc acgagacaaa tgccactatt cctgctcgac gctaaacgcc gatttggagg
     2341 cactcatcct caaatcgagg gtctagtgcc ggtgacagcc cagcgccgat ccggaaatca
     2401 ggtccactac tccgccgcgg tcaccgtgcc tgcgggcgcg ctcgcccccg cgccgtagcg
     2461 ctggtcgatg tagtcgatca ccatcgcctt gaattcggtg gcgatgttgg gcccgcgcaa
     2521 agtgcggaat ttcttgccgt cgacgaatac cggcgccgcc ggcgtctcgc cggtaccggg
     2581 caaggagatg ccgatgtcgg catgcttgga ctcgccgggg ccgttgacga tgcagcccat
     2641 gatcgcgacg ttgagcgatt cgacgccggg atagcgcgtc ttccagctcg gcatttcgtc
     2701 gcgaatgaaa tcctggatcg agcgcgccag ttcctggaag gtggtcgagg tggtgcggcc
     2761 gcagccgggg catgccgcga ccagcggcac aaaggtgcga aaacccatgg tctgcagcag
     2821 ttcctgcgcg acctgcactt ccagcgtgcg atcgccgccg ggctccggcg tcagcgaaat
     2881 ccggatggtg tcgccaatgc cgtcctgcag caggatgccg agtgccgcgg ccgaggccac
     2941 gatgcccttc gatcccatgc cggcttcggt gaggccgaga tggatcgcgt aacccgaacg
     3001 cgaggcgagc gtttgataga ccgcgatcag gtcctgcacc gccgagactt tcgccgacag
     3061 gatcatcttg ttcttcggca ggccgatctc ctcggcacgc gcggccgaca acagcgccga
     3121 ctgcaccatc gcctcgcgcg tcaccgcgcg gacatccttc ggctcggcga gcagcgcgtt
     3181 ttcgtccatc agtctggtca gcagttcctg atcgagcgaa ccccagttcg cgccgatccg
     3241 aaccgccttg ttgttcttga gtgctatctc gacgatgtcg gcgaactggc tgtcgcgctt
     3301 gttcttgaag ccgacattgc cgggattgat gcggtatttg tcgagcgcct cggcgcaggc
     3361 cggatagtcg gccaagagct tgtggccgat ataatggaaa tcgccgatca gcggcgtggt
     3421 gatgccgcgc ttgcgaagtt cgtcgcggat atgcggcacc gcggccgctg cctcgtcgcg
     3481 gtcgaccgtg atccgcacca attcggaacc ggcgcgcgaa agtgctgcga tctgcgccac
     3541 agtgcccgcg acgtcggcgg tgtcggtgtt ggtcatcgac tgcaccacga tcggcgcgcc
     3601 cccgccgacg gcgacattgc cgaccatgac ctgggtggtt ttacgccggg cgttggggcc
     3661 ggcgacatcg tcttggggtg gttttactgg ctggttcatg gggtctcaaa tatcaggttt
     3721 tggtgacatt caccacgggg ccggcggcag gtctgtgcgc atcatcacgg tttggacggt
     3781 ttggaatttt tcgtccattt attcaatagc tt
//
LOCUS       DASWGM010000658         6057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2440800,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000658 DASWGM010000000
VERSION     DASWGM010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2440800"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1427
                     /locus_tag="VGA15_18480"
     CDS             294..1427
                     /locus_tag="VGA15_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9589718.1"
                     /translation="MDLAEQLSELVGEIYDAALDPALWCDVVGKAGRFVGGSAAAIYA
                     RSPTALTGNVYYESGTDPHYRELYFNKFIKFDPTTIGHYFADVEQPIATADIIPYPEF
                     LETRFYKEWARPQGWVDTVNAVLDRSVTSAASFVVFRHQRDGVVDDETRRRMRLIVPH
                     IRRAVLIGRLIDLRAMDAANLADTLDGLSAAMCAVDADGRIVHANVACHIILEARDFL
                     SVIGGRLVASDAKIDRTFRELFAAAGSGDAAVGTRGIALPLRAKDGSHYVAHVLPLAS
                     GARSLAGIASSATAALFICKVAAEIRSSPEAIARAYDLTPTELRVLLAIVDVGGVPES
                     AVALGIAESTVKTHLANLFVKTRARRQADLVKIVAGFASPLIG"
     gene            1697..2830
                     /locus_tag="VGA15_18485"
     CDS             1697..2830
                     /locus_tag="VGA15_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9589719.1"
                     /translation="MDQAEQLFELIGEIYDAALNPSLWSDVVGKAGRFVGGSAAAIYA
                     KSPTAMTVTVHYQSGIDPYYLQIYLDKYVKLDPTMIAHYFADVEQPIATADIIPHHEL
                     LETRFYKEWVQPQGIVGNLSAVLDKSATSVALFGVFRYDREGVVDDEARRRMRLIVPH
                     VRRSVLIGGLIDLRSADAANLADTLDGLSAAMCAVDADGRIVHANAACHVLLDAGDFL
                     SAVGGRIVASDAKIDRTFRELFAAAGSGDAAIGTRGIALPLRAKDGSHYVAHVLPLTS
                     GARRLAGIAYSATAALFIRKAATEIRSPPEVIARAYHLTPTELRVLLAIVDVGGVPES
                     AVALGIAESTVKTHLGNLFVKTGARRQADLVKIVAGFASPLIG"
     gene            3338..4474
                     /locus_tag="VGA15_18490"
     CDS             3338..4474
                     /locus_tag="VGA15_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9589720.1"
                     /translation="MDQAEQLSSLIGEIYDAALDPSLWSDVLGKAGRFVGGPVAAIFA
                     KSPTALTGTVYYHSGGKDPSYRQLYFDKYIKFDPTTTAQYFSDVEQPMAVADIMPYEE
                     FLETRFYKEWVQPQGMVDAVTAVLDKSATSAALFAVFRYQSEGIVDDETRQRMRLIVP
                     HIRRAVLIGRLIDLRAADAASLADTLDGLSAAMCAVNADGRIVHANAACHVLLDAGDF
                     LSVVRGRIVAREAKVDQTLRELCAAAGGGDAAIGTRGIALQLMARDGSRYVAHVLPLT
                     SGARRITGIAYSATAALFICKVATEAPSAPEVIARAYHLTPTELRVLLAIVDVGGVPE
                     SAVALGIAESTVKTHLGRLFVKTGARRQADLVKIVAGFASPLIG"
     gene            4691..5605
                     /locus_tag="VGA15_18495"
     CDS             4691..5605
                     /locus_tag="VGA15_18495"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9589721.1"
                     /translation="MKRIVGGMVVAAALSGSAFATDLPLPKYTKAPAITKAPAIAAPA
                     YNWSGWYGGLNAGWVGGAGSVSNNASIVSNDIPEVAEAMARGATNTAGASSGFIGGGQ
                     FGYNYQFSHSFVAGFEADIQGLTGTHKRSSSAALFTVPASEGEDRASIVTNSTTTRDL
                     SYLGTLRARIGVLVAPSFLLYATGGLAYGGAKSDTTIAQSVTITDTNPLPSTLTSGSF
                     SQTRVGYAVGAGGEWMLSSNWSAKLEYLYYDLGSATYTTGGIANDPTGLPHDGTAAVA
                     TSSKVRFNDNIVRVGVNYHFGGPAVSQY"
BASE COUNT         1098 a         1822 c         1857 g         1280 t
ORIGIN      
        1 ccaatccgct gtcgcgtacg tccaagcggg acgccaatgc tgcgtgtaaa atcccgatcg
       61 tgccggaagc ttccgcgctg gatggcctgt tggcgaacgc tggcgtcggg caggtgcggg
      121 gtcctttcgt gccgcgcttg ccaaaccggc gccttgcgtt tggcacatcg agccagcagc
      181 gcaaggcaat tcggttgaat gcaattcggt tgaaggcaat tcggttcaag gcaattcggc
      241 ttcggttgcg tcgacggtcg cccgtgcggg ggtgtccgac ttcggggatg accatggatc
      301 tggcggagca gctttctgaa ctcgtcggcg agatctacga tgccgcgctg gatcccgcgc
      361 tttggtgtga cgttgtcggc aaggccgggc gtttcgtcgg cggttcggcg gcggcaatct
      421 atgccaggag cccaaccgcc ttgacgggta atgtctatta cgagtccggc actgacccgc
      481 attatcgcga actctatttc aacaaattta tcaaattcga ccctaccacg atcggccatt
      541 acttcgctga tgtcgagcaa ccgatcgcga cagcggatat cattccttac ccggagttcc
      601 tggaaacgcg gttttacaag gagtgggccc gaccgcaagg ctgggtggat accgtgaacg
      661 cggtgctcga tagatcggtg accagcgcgg catcgttcgt ggtgtttcgc caccaacgcg
      721 acggcgttgt cgacgatgaa acgcgacggc ggatgcgcct catcgttccg cacattcgcc
      781 gggcggtgct gatcggcaga cttatcgatc tcagggctat ggatgctgcg aacctcgctg
      841 acaccctcga tggcctcagc gcggcgatgt gcgctgtcga tgcagatggg cgcatcgttc
      901 atgcgaacgt cgcctgccac atcattctcg aggcccgcga tttcctgtcc gtgatcgggg
      961 ggcggctcgt cgcaagcgat gcgaagatcg accggacttt tcgtgagctg tttgcagctg
     1021 ccggcagcgg cgatgcggcg gtcggaaccc gggggattgc cttgccgctg cgggcgaagg
     1081 acggctcgca ttatgtcgcg catgtgctgc cgttggcatc cggcgcccgc agcctggccg
     1141 ggatcgcttc ctccgcgacc gcggccttgt tcatctgcaa ggtcgcagcg gaaatccgat
     1201 cttctccgga agccatcgcc cgcgcctatg atctgacccc gaccgagttg cgcgtgctgc
     1261 tcgccatcgt cgatgtcggc ggggttcccg aaagcgcggt ggccctcggc attgccgaaa
     1321 gcacggtcaa gacgcacctc gccaacttgt ttgtgaaaac ccgcgcccgt cgccaggccg
     1381 atcttgtgaa aatcgtcgcg ggatttgcca gtccgctgat cggctaacgg atgtcatcga
     1441 cagatcccgg ccgccgacca ctggccgaga gtcatttcga ttctgcgcgc ggtcgcctcc
     1501 gtccagcctc cgcgttgtcg cggcacccgg atcgaacctc aatttcgatc cggtgtgggc
     1561 gacgcaggtt cgccgacccc gttccgcggt ccgaccttgg ctatctcgtc aactccttgc
     1621 gtgaagtgcc gggcacacgg ggcggatcgc gttgcgcgag aaggcgacgt tcaagtgtcc
     1681 gtcttcgggg atgaccatgg atcaggcaga gcagcttttt gaactcatcg gcgagatata
     1741 tgatgcagcg ctgaatccgt cgctgtggag cgacgttgtc ggcaaggccg ggcgtttcgt
     1801 tggtggctcg gcggcggcga tctatgccaa gagccccacc gccatgaccg tcacagtcca
     1861 ttatcaatcc ggcatcgacc cgtattatct acaaatctat ctcgataaat atgtgaagct
     1921 cgaccctacc atgatcgccc actacttcgc tgatgtcgag caaccgatcg caacggcgga
     1981 tatcatacct caccatgagc tcctggaaac gcgattttac aaggaatggg tccaaccgca
     2041 aggcatagtg ggtaatttat cggccgtgct cgacaaatcg gcgaccagcg tggctctgtt
     2101 tggggtgttt cgctacgatc gggagggcgt tgtcgacgat gaagcgcggc ggcgcatgcg
     2161 cctcatcgtt ccgcacgttc gccggtccgt gctgatcggc ggacttatcg atctcaggtc
     2221 cgcggatgct gcgaacctcg ccgacaccct cgatggcctc agcgcggcga tgtgcgctgt
     2281 cgatgcagat gggcgcatcg tccacgccaa tgccgcctgt cacgtccttc tcgacgccgg
     2341 cgatttcctg tccgcggtcg gggggcggat cgtcgccagc gatgcgaaga tcgaccggac
     2401 ttttcgtgag ctgtttgcag cggccggcag cggcgatgcg gcaatcggaa cccggggtat
     2461 tgccttgccg ctgagggcga aggacggttc gcattacgtc gcgcatgtgc tgccgctgac
     2521 atcgggcgcc cgccgcctgg ccgggatcgc ttactccgcg accgcggcct tgttcattcg
     2581 caaggcggca acggaaatcc gatctcctcc ggaagttatc gcccgcgcct accatctgac
     2641 cccgaccgag ttgcgcgtgc tgctcgccat cgtcgatgtc ggcggggttc ccgaaagcgc
     2701 ggtggccctc ggcattgccg aaagcacggt gaagacgcac ctcggcaatt tgtttgtgaa
     2761 aaccggcgcc cgtcgccagg ccgatcttgt gaaaatcgtc gcgggatttg ccagtccgct
     2821 gatcggctaa cggatgtcgt cgatagaccc ccggccgccg accactggcc gagagtcatt
     2881 tcgattcagg gcgcggtcgg cctccgtcca gcctccgcgt tgtcgctgcg ccccggatcg
     2941 aacctcgatg tcgatccggc gtgagcgacg caggttcgcg gacaccgtgc ctcaatccga
     3001 ccttcgttgt cccgtcaact ccttgcgtga gctgccgggc acacggtgcg gatcgggttg
     3061 cgcgagaagg ccaattcgtt cgcccacaaa cgcaagtgac catcgtgagc gggagttctg
     3121 tgctcttggt cacagaaccc gggcttgcgg ctcgccaata atactagagc gttttcaagt
     3181 gaagtgatac cggttcgcgt caagaaaacg cgtcaaatca ataatttaga gccccgtttc
     3241 ggttctatcg aaacggaaaa ggctctcggg gttcaaggta attcggcggc aacgaagtcg
     3301 acgaccgacg ttcaagtgtc cgtcttcgag gatgaccatg gatcaggcag agcagctttc
     3361 gtcactcatc ggtgagatct acgatgccgc gctggatccc tcgctgtgga gcgacgtgct
     3421 cggcaaggcc gggcgtttcg tcggcggccc ggtggcggca atctttgcca agagcccaac
     3481 cgccttgacc ggcactgtct attatcattc cggcggtaag gacccgtcct accgacagct
     3541 ctatttcgac aaatacataa aattcgaccc caccaccacc gcccaatact tctctgatgt
     3601 cgagcaaccg atggcagtgg cggatatcat gccttacgag gagttcctgg aaacgcggtt
     3661 ttacaaggag tgggtccaac cgcaaggcat ggtggatgcc gtgacggcgg tgctcgataa
     3721 atcggcgacc agcgcggcat tgttcgcggt gtttcgctac caaagcgagg gtattgtcga
     3781 cgatgaaacg cgccagcgca tgcgcctcat cgttccgcac atccgccggg cggtgctgat
     3841 cggcagactt atcgatctca gggccgcgga tgccgcgagc ctcgctgaca ccctcgatgg
     3901 cctcagcgcg gcgatgtgcg ctgtcaatgc ggacggacgc atcgtccacg ccaatgccgc
     3961 ctgtcacgtt cttctcgacg ccggcgattt cctgtccgtg gtcaggggac ggatcgtcgc
     4021 gagagaggcg aaggttgatc agaccttgcg ggagctgtgt gcggctgcgg gtggtggcga
     4081 cgccgcaatc ggaactcggg gtatcgcctt gcagctgatg gcgcgggacg gctcgcgcta
     4141 tgtcgcgcat gtgctgccgc tgacatccgg cgcccgccgc attaccggga tcgcttactc
     4201 tgcgaccgcg gccttgttca tttgcaaggt ggcaacggaa gccccttctg cgccggaagt
     4261 catcgcccgc gcctaccatc tgacccccac cgagttgcgc gtgctgctcg ccatcgtcga
     4321 tgtcggcggg gttcccgaaa gcgcggtagc cctcggcatt gccgaaagca cggtcaagac
     4381 gcacctcggc aggttgtttg tgaagaccgg cgcccgtcgc caggctgacc ttgtcaagat
     4441 cgtcgcgggt tttgccagtc cgctgatcgg ctagctcaaa tcgtccccgt catccctgca
     4501 aggcgtgtgg ctaatccgca tcatccgccg cgaatatctc tgggctgcgt tctcgtctta
     4561 ttgaagcgcg ccgccgtcca agccgcaaat ccccgtcgca cataggacta gcggatatcg
     4621 aatctcgctt gtgagattca tgttgcttgg cggaacggct cgcgcggcgg ataaattcga
     4681 ggagatttca atgaagcgga ttgtgggcgg aatggttgtg gcggcggcgc tatccggttc
     4741 ggcgttcgcc accgatcttc cgttgcccaa gtatacgaaa gcacctgcga tcacgaaagc
     4801 acctgcaatc gctgctccag catacaactg gagtggctgg tacggcggcc tgaatgcggg
     4861 ttgggttggt ggggctggca gcgtgagcaa caatgccagc attgtttcaa atgacattcc
     4921 cgaagtcgcg gaagctatgg cccgtggcgc aacgaatacg gccggtgcat cgagcggctt
     4981 catcggcggc ggccagttcg gatataatta tcagttctcg cactcgttcg tcgcaggctt
     5041 cgaggcggat attcaggggc tgaccgggac gcacaaacga tcgtcgtcgg ctgcactttt
     5101 cactgtcccg gctagcgaag gagaggatcg cgcttcgatc gtcacgaact caacgaccac
     5161 gcgcgatcta tcttaccttg gaacgttgcg cgctcgtatt ggtgttttgg tcgctccgag
     5221 ctttttgctg tatgcaacag gtggcttggc gtatggcgga gccaagtccg acaccacgat
     5281 cgctcaatcc gtaaccataa ccgataccaa tcctcttccc tccaccctga catcggggtc
     5341 gttttcgcaa acccgcgttg gatacgccgt gggtgctggc ggcgagtgga tgctttcctc
     5401 caattggagc gcaaagcttg aataccttta ctacgatttg ggatccgcaa cctacacgac
     5461 gggaggtatc gcaaacgatc cgacaggcct tcctcacgac ggtacggctg ctgtcgcgac
     5521 gtcttcgaag gtccgcttca atgacaacat cgttcgcgtc ggcgtgaact atcatttcgg
     5581 aggcccggcg gtttcgcaat actgatcgtc tcgctattgc cagcgaagag cccggccacg
     5641 tgccgggctt tttgttggtg tgccgagcat gatcgagagc ttggaggtgt cagtcctcta
     5701 tccagcctga tggcggtgtg gcgcgttcgt actcgatgaa ttgccatggc ttcaattttc
     5761 aaaaagcaaa ttccagcaat aacttcgccc tcgcgacgcg aagcgcccga ggcgttgctg
     5821 aatcgtccgc cccaaaagag ggcgtgggga atgccgggtg cccgttgcac ccgcagcctc
     5881 gtgcgcgctt ggcattggta aaaatgcaca cgagtaaacg agtacaccgg aatcgcccgg
     5941 cattcccgca cgcaatggtt ttaacggtag agtaggagtg tggcgcagtg aacgttaggc
     6001 ccggcgtatc tgtttccgcc cctttcgtct ggcggtgcct gagcggccga gccatgg
//
LOCUS       DASWGM010000659         7763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2440977,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000659 DASWGM010000000
VERSION     DASWGM010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2440977"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VGA15_18500"
     CDS             <1..539
                     /locus_tag="VGA15_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589722.1"
                     /translation="RATVSQITPKGDPTRKTFRVYLRLPPDTPLRIGMTVEVNIIYRE
                     KTAAVVVPAEAVAGNAVQVVSSGKIQRVPVTVGVRGSRNVEIIGDVSKDTTVLSPARA
                     DLADGTRIRVENVAAKPAPAAPADIAPVPSPALDRAPAAAVVASAADADDAVISAAIS
                     AHVDSVVNDARRNVGKYR"
     gene            545..1789
                     /locus_tag="VGA15_18505"
     CDS             545..1789
                     /locus_tag="VGA15_18505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589723.1"
                     /translation="MATNLLLSIAWTHVTTRIRQTLVGMCGVAMGVGFTIMMAGLMQG
                     SQIDFLRQLVDTMPHVTVGDERRSVPSQPADQEYAAVGMPNVANVNRRPGIKYPDTVM
                     ASLKSWLPGDVAPSVKTTAILNHGDARVGVSLIGIDPRLEVRVSKLGSQMLEGQLTDL
                     SKAANGIIIGAALAEKLAVKYGNTVLLGTGNGTPISATVVGIFKSGIKSVDEGQIYSL
                     MNVAQTLIGQNGLVNQLRVRLSDPLIAQQVATRVEAQIGYKSVSWQEANSDLLGAFAT
                     RDFIVLTVMGAMLLSSSFATYNIISTITHEKRHDIAIMKSLGMREYSVRRIFIIESTM
                     MGSVGILFGWLLGYLLCYGWSQIKIFNVLTGATVPLEIYYSPTHYLATGAISIFCCAG
                     AAFFPARKATRVHPVEIIRGAS"
     gene            1795..2526
                     /locus_tag="VGA15_18510"
     CDS             1795..2526
                     /locus_tag="VGA15_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589724.1"
                     /translation="MALQTVKLVRRVQGIVSHTLVDGIDLSVAKGEFLAITGPSGSGK
                     SSLLYLLGLLDAPTEGEVIICGQATSKLSETERADVRLTKCGFVFQFHFLLPEFTALE
                     NVLLPMRAYGRMSTKEMNERAMSLLTSLGLGEQTGKRPNQLSGGQRQRVALARALANR
                     PEIIVADEPTGNLDTASTEQVFGILRDIANSGQTVVVVTHDAALAARADRRIHIVDGK
                     IHETTFGGDRKPEIAAVTPAAECPS"
     gene            complement(2554..2778)
                     /locus_tag="VGA15_18515"
     CDS             complement(2554..2778)
                     /locus_tag="VGA15_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589725.1"
                     /translation="MRRIERFSPIYDASRLDDAAKKAENAARADLQAQMSAILGELNR
                     RGFGPAALNLSTFTGATRQAQELLAAAMRR"
     gene            complement(2923..3732)
                     /locus_tag="VGA15_18520"
     CDS             complement(2923..3732)
                     /locus_tag="VGA15_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX9589726.1"
                     /translation="MTKLFAVAAFLLASTAAQAQYTFEYGGRTIRIDPDRGTVSIPGV
                     YDNTGRRTKRSHNDQDSDRPRKQAPQQAKTDPQAPEAAPAQTEPAPAVANTSPEPSTA
                     TATAPARVAPTTDTITSTQPAASTPAPRQDAAPAIRPEPAPVVATTAPASAPIQNQAP
                     APATTAVQSANSPLGMWLTEEKEGKVRIEQCGANLCGYSVDAKSNQNGEKVLINMKPT
                     KDSKWAGRIFDPNTGSTYDSTIALKGSDNLRVQGCAFGGMFCGGQTWSRLN"
     gene            3985..4160
                     /locus_tag="VGA15_18525"
                     /pseudo
     CDS             3985..4160
                     /locus_tag="VGA15_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495205.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 family protein"
     gene            complement(4195..4599)
                     /locus_tag="VGA15_18530"
     CDS             complement(4195..4599)
                     /locus_tag="VGA15_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589727.1"
                     /translation="MSKRDPTDHALAAIASILDQPLFHREPEKPAVEEKPIAPELVEA
                     GGYQKVGPGPIAAIRFKWTVRRDGHNQYYVDETIGENSVPVVSGPLTGDAAIKFVDDR
                     ESEARQRFEDLKSEMAGRTAAANLVRKDAGEI"
     gene            complement(4690..6114)
                     /locus_tag="VGA15_18535"
     CDS             complement(4690..6114)
                     /locus_tag="VGA15_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX9589728.1"
                     /translation="MAFKEYGSYDGMGLADLVRKKQISAGELLDEAIARTAKVDPQIN
                     AVVVKHCDYAQRQIDRGLPDGPFTGVPFLLKDLDILEGTRTTSGASVYKDNVADHTGT
                     LAQRFLNTGVTIFGKSSSPEFGLMPTTESRLFGPTRNPWNPAHSSGGSSGGAGAAVAA
                     RILPVAHASDGGGSIRIPASASGVFGLKPSRARNPLGPDRGEGWGGFSCGHVLSISVR
                     DSAAMLDAIHGPEPSSLYVAPPPERPFIEEVGRDPGRLRIAFTDRSPYGDAIDPEIAA
                     AVRDVTNLLAGLGHHVEERAPVLAADPAVVMSTIVAANTALSVRQAEARFGRTMTESD
                     FEILTLASAHNAQNATATDYVAAQLAAFQISRGLAMFFETCDVFLCPTLCSPPLRIGE
                     LNTMSQDLSHIPPILRRYMPGTAMFNMSGQPAMSVPLAWNAAGLPLGMMFAARLGDEA
                     TLFRLAAQLEQERPWRDKLPPICA"
     gene            complement(6174..7163)
                     /locus_tag="VGA15_18540"
     CDS             complement(6174..7163)
                     /locus_tag="VGA15_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX9589729.1"
                     /translation="MATNKKKIFITESMSQQGRALLKERDDIEIVEFPNMISAHDFES
                     MLRAQAPVHGVALGGTRFGEPELEASKDMKVVTRIGVGYDAVDVPALSRRNIPLMVAG
                     TANSPSVAEQALFMMLMLAKRAVEMHAIVKDGKWTHRLGMLPYDLFGKTVLIIGFGRI
                     GTRTAKRCLAMEMNVLVFDPYKPAAEITAAGCEPVTGLDAALPRADFVSIHCPKTPET
                     VGMFNAARLKRMKETAYLINTARGGIVDEPALYDALVSGKLAGAGLDVFEQEPPPSGH
                     SLFELANVIMAPHVAGVTREAVDRMSVQTARNILSALDGEPIRQNVINQDVLA"
     gene            complement(7208..>7763)
                     /locus_tag="VGA15_18545"
     CDS             complement(7208..>7763)
                     /locus_tag="VGA15_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX9589730.1"
                     /translation="VRTTTVYVTHDQVEAMTLADRVVVMNHGRIEQIGTPNDLYHNPA
                     TRFVAGFIGSPAMNFMLCGLEDVAGKLHIRLTDRIAFPLPANRAARYQGVTRNEKLLL
                     GLRPEHITEAKAHPGPGIEAFDAVLDVTEPMGMETLIYFTLEGAQICGRVNPNAGARD
                     GAPLRLAVDLNNMHLLNEVTGAVI"
BASE COUNT         1422 a         2381 c         2388 g         1572 t
ORIGIN      
        1 tgcgggccac ggtttcgcaa atcacgccaa agggcgatcc cacgcgaaag accttccgtg
       61 tctatctgcg attgccaccc gatacgccat tgcggatcgg aatgacggtc gaagtcaaca
      121 tcatctatcg cgaaaagacc gcggcggtcg tcgtgcccgc cgaggccgtt gccggcaatg
      181 cggtccaggt cgtcagtagc ggaaaaattc agcgggtgcc cgtcaccgtc ggggttcgag
      241 gcagccgtaa cgttgaaatc atcggcgatg tgtcgaagga caccaccgtg ctgtcaccgg
      301 cacgcgccga cttggccgat ggaacgcgaa tccgcgtgga aaacgtggcc gccaagccgg
      361 caccggccgc tcccgcggat atcgcgcctg ttcccagtcc tgctcttgat cgcgcacccg
      421 ccgccgccgt ggttgccagt gctgccgatg ccgacgacgc cgtcatatcc gccgccatat
      481 cggctcacgt cgattccgtt gtgaacgatg cacgcaggaa cgtcggcaag tacaggtaaa
      541 acccatggcg acgaatcttt tgctgagtat cgcgtggacc catgtgacca ctcgcattcg
      601 acaaacactg gtcggcatgt gtggcgtggc gatgggtgtc ggcttcacca tcatgatggc
      661 cggcctgatg cagggatcgc agatcgattt tctgcgacaa ctcgtcgaca ccatgcccca
      721 cgtcacggtt ggtgatgagc gtcggtcggt tccgagccag ccggcggacc aggaatacgc
      781 cgcggttggc atgcccaatg tggcaaacgt caatcgacgc cccggcatca aatatccgga
      841 tacagtcatg gcgtcgctga agtcgtggtt gccaggcgac gtggcgccgt cggtgaagac
      901 cacggctatc ttgaaccacg gcgacgcacg ggttggcgtc agcctgatcg gcatcgatcc
      961 gcggcttgaa gttcgcgtct ccaagctcgg ctcgcagatg ctcgaagggc agctgaccga
     1021 tctgtcaaag gcggccaacg ggatcatcat tggcgcggcg ctggcggaaa aacttgccgt
     1081 gaaatatgga aataccgtct tgctcggcac gggcaacgga actccgattt cagccacggt
     1141 ggtcggtatc ttcaagtcgg gaatcaagag cgtcgacgaa ggacaaatct attcgttgat
     1201 gaatgtggcc cagactctca tcgggcagaa cggactcgtc aatcagttgc gggtgcgcct
     1261 gagcgatccc ttgatcgcac agcaagtggc cacgcgcgtc gaggcacaaa tcggatacaa
     1321 gtcagtgtcg tggcaggagg ccaactccga tctgctgggt gccttcgcta cccgtgattt
     1381 cattgtcctg accgtgatgg gcgccatgct gctgagctct tcattcgcga cttacaacat
     1441 catttccacc atcactcatg aaaaacgcca cgacatcgca atcatgaaat cgctcggcat
     1501 gcgggaatac tcggtgcggc gaatattcat cattgagtcg acgatgatgg gatcggtcgg
     1561 aatcctgttc ggatggcttc tcggatatct cttgtgctac gggtggtctc agatcaaaat
     1621 attcaatgtg ctgaccggag caacggtacc cctcgagatc tactattcgc cgacgcatta
     1681 tcttgccacc ggagccattt ccattttctg ctgcgccggc gcggccttct ttccggcacg
     1741 caaggccacg cgcgtgcatc ccgtcgaaat tatcaggggt gcgtcgtgac tgagatcgcc
     1801 ctgcaaaccg tgaagctggt ccgccgcgtt cagggcattg tttctcacac cctcgtcgat
     1861 ggtatcgatc tatccgtggc gaagggcgaa tttctcgcca tcaccggccc atcgggatcc
     1921 gggaaatcat cgctgctcta tttgctcggc ctgctcgatg cgcctaccga aggcgaggtc
     1981 atcatttgcg gccaggcaac gtccaaattg tcggaaaccg agcgcgccga tgtccgtctg
     2041 accaagtgcg gcttcgtgtt tcagttccat tttctgcttc ccgaattcac cgcgcttgaa
     2101 aacgtgctgc tgccaatgcg cgcttatggc cggatgtcga ccaaggaaat gaacgagaga
     2161 gcgatgagcc tgctgacgtc gctcggcctc ggcgagcaga ccggcaagcg gcccaatcag
     2221 ctttccggcg gtcagcggca acgcgtggcg ctggcgcggg cgcttgccaa ccgtcccgaa
     2281 atcattgtcg ccgacgagcc gaccggaaat ctcgacactg cttcgaccga gcaggtgttc
     2341 ggcatcctgc gcgatatcgc caacagcggc cagaccgtgg tggttgttac ccacgacgcg
     2401 gcactggccg cgcgggccga ccggcgcatc catattgtcg acggcaagat ccacgagacg
     2461 acgtttggcg gcgaccggaa gcccgagatc gcagcagtga ccccggcggc agaatgtccg
     2521 tcgtgaccgc aagcgagttc gcgagccgtt cggctacctt cgcatcgccg ccgccagcag
     2581 ttcctgcgcc tgcctcgtcg ccccggtaaa tgtcgagaga ttgagcgcgg ccgggccgaa
     2641 gcccctgcga ttgagctcgc ccaggatcgc gctcatctgc gcttgaagat cggcgcgtgc
     2701 ggcgttctcg gccttcttcg cggcatcgtc gagcctgctt gcgtcgtaga tcggcgagaa
     2761 tcgttcgatt cgccgcatcg cgcatcgcaa tgtcgcgatc ctcgggcgaa gggctcaacg
     2821 gcgcggcgtc ggcttcgaga gcatcacaac gagacactca accgcgcgcg ccgcctgggc
     2881 agtagcggcg ttgctcacgg gttagattta tgagtacacg ccctaattca gccggctcca
     2941 ggtctggccg ccgcagaaca tgccgccaaa ggcgcaaccc tgaacccgca ggttgtccga
     3001 gcctttcaga gcgatcgtcg aatcgtaggt gctgccggtg ttcggatcga aaattcgccc
     3061 ggcccatttc gagtccttgg tgggcttcat attgatcaga actttttcgc cgttctggtt
     3121 cgacttcgcg tccacggaat atccgcagag attggcgccg cattgctcga tccggacctt
     3181 gccctccttc tcttccgtca gccacatgcc gagcggcgag ttcgccgact ggacggccgt
     3241 ggttgcgggg gcaggggcct ggttctggat cggtgccgat gccggggctg tcgttgccac
     3301 gacaggggcc ggttcgggcc ggatggccgg ggcagcgtcc tgccgcggcg caggcgtgct
     3361 ggccgcaggc tgcgttgacg taatggtatc cgtcgtgggc gcaacgcgcg ccggagcggt
     3421 tgccgtagca gtcgaaggct cgggtgaagt gttcgctaca gcaggagccg gttcggtctg
     3481 ggccggcgct gcttcgggag cctgcggatc ggtcttcgcc tgctgcggcg cctgtttgcg
     3541 cggacggtcg ctgtcctgat cattgtgcga gcgctttgtt cgccgcccgg tgttgtcata
     3601 gacaccgggg atcgaaaccg ttccacggtc cggatcgatg cgaatggtac gcccgccata
     3661 ttcgaacgtg tactgcgcct gcgcggcggt gctcgccaac aggaatgcgg cgacagcaaa
     3721 cagctttgtc atagtgacct cctgggcagg cagaatctca aggccccacc ctgcacttcg
     3781 gtagttttcc agaacgtgac ctacatcact ttcttggtat aagtcggtgg attgccggct
     3841 ttggttcaat gtgtgtcggc ggccaatcta attcaggtcc gcatgggcgg tcaattttcc
     3901 gaaacaaaac cgctccaaaa ccggtcattg tgcggcaatg gcccgttgca aaacgtgggg
     3961 atagatttaa cgccaaaatc gggcaggttg cgcaatggtt ccaggcgcaa atttaccggt
     4021 caatccctgc tgctgacttt cagcgtaccg cagtttcttt tttttacgtc acgacggcct
     4081 atgacatctt gcgccattgc ggcgtcgatc tcgccaagaa ggactttctg ggaacgccgg
     4141 atcgagcgtc acaagactga ttggtcgaga ctgattgggt cgagaccgat tgagttatat
     4201 ctcgccggcg tctttgcgga ccaaattcgc cgcggcggtt cgtccggcca tttcgctctt
     4261 gaggtcctca aatcgctgcc gggcctcgct ttcgcgatcg tcaacgaact tgatggcggc
     4321 atcaccggtc aatggcccgc tgacaacagg cacggaattc tcgccgatcg tctcatccac
     4381 ataatattgg ttatgaccgt cgcggcggac cgtccatttg aatcggatcg ctgcaatcgg
     4441 gccaggaccg accttctgat aaccaccggc ctcgaccagt tcgggggcga tcggcttttc
     4501 ctcgactgcg ggtttttccg gctcgcgatg gaacagcggt tgatcgagga tgctggcgat
     4561 agccgcgagc gcatgatcgg tcgggtcgcg tttactcaca atctcagcct ccagattacg
     4621 caatacacgg cttcgctgtt agcatagggt tccattcctc gccctcgaat gcggctgagt
     4681 tttgtcgatt caggcgcaaa tgggcggcaa tttgtctctc cagggccgct cctgttcgag
     4741 ttgggccgcc aggcggaata aggtggcctc atcgcccagg cgcgcggcga acatcattcc
     4801 gagcggcagt ccggccgcat tccatgccag cggtaccgac atcgcgggct ggccggacat
     4861 gttgaacatg gcggtcccgg gcatatagcg ccgcaggatc ggcgggatat gcgacagatc
     4921 ctgcgacatc gtgttgagct cgccgatccg caagggcggc gagcagagcg tcgggcacag
     4981 gaacacatcg caggtctcga agaacatcgc caatccgcgt gaaatctgga atgcggcgag
     5041 ttgcgcggcc acatagtcgg tcgcggttgc attttgcgcg ttgtgtgcgc ttgccagcgt
     5101 caatatctcg aaatcactct ccgtcatcgt gcggccaaag cgcgcttcgg cctggcgcac
     5161 gctcagcgcg gtattggccg ccacgatggt cgacatcacg accgccggat cggcggccag
     5221 cacgggcgcc cgttcctcga catgatgacc gagcccggcg agcagatttg tcacgtcccg
     5281 aaccgcggcc gcgatttcgg ggtcgatggc gtcgccatag ggcgatctgt cggtaaaggc
     5341 gatgcgcagc cggcccggat cgcgtccgac ctcttcgatg aaaggtcgct ccggcggcgg
     5401 cgcgacatag aggctcgagg gctcaggtcc gtggatcgcg tccagcatcg cggcgctgtc
     5461 gcgcacgctg atgctcagca cgtggccaca ggaaaagccg ccccagcctt cgccgcggtc
     5521 gggtccgagc ggattgcgcg cccggctcgg tttcaatccg aacacgccgg acgcggaagc
     5581 cgggatgcgg atcgaaccgc cgccgtcgct ggcatgcgcg acgggaagaa tccgcgcggc
     5641 gacggcagca ccggcgccgc cggacgaacc gcccgaggag tgggcgggat tccaggggtt
     5701 gcgggtcgga ccgaacagcc gggattcggt ggtcggcatc aatccgaatt ccgggctgga
     5761 gctttttccg aagatcgtca ccccggtatt gaggaagcgc tgggcgaggg tgccggtatg
     5821 atcggcaaca ttgtccttgt agacgctcgc gcccgaggtc gtccgcgtgc cctccagaat
     5881 gtccaggtcc ttcaagagaa acggcacgcc ggtgaacggg ccatccggaa gccccctgtc
     5941 gatctggcgc tgcgcatagt cgcagtgttt caccaccacc gcgttgatct gcggatcgac
     6001 tttagccgtg cgggcaatgg cctcgtccag caattccccg gcgctgatct gcttcttccg
     6061 gaccagatcg gctagaccca ttccgtcata gctgccgtat tccttgaaag ccatttggac
     6121 acccgcggtg atgatgcaac gaagccagcg aagtcggcgg cgtgccgctc agatcaggcg
     6181 agcacgtcct gattgatgac gttctgcctg atcggttcgc cgtcgagcgc gctcaagata
     6241 ttgcgcgcgg tctgcacgct catccggtcg acggcttccc gcgtcacgcc ggcgacgtgc
     6301 ggggccatga tgacattggc aagctcgaac agcgaatggc ctgagggcgg cggctcctgt
     6361 tcgaaaacat cgagaccggc gcctgcgagc ttgcctgata ccagcgcgtc ataaagcgcc
     6421 ggttcgtcga cgatcccgcc gcgcgcggtg ttgatcagat aggccgtttc cttcatgcgc
     6481 ttcaatcggg ccgcattgaa catgccgacc gtctccggcg tcttcgggca gtggatgctg
     6541 acaaaatcgg cgcgcggcag cgccgcatcg agaccggtca ccggctcgca gccagccgcc
     6601 gtgatttcgg cggcaggttt gtagggatca aagaccaaaa cattcatttc catggcgagg
     6661 cagcgtttgg cggtgcgcgt gccgatgcgg ccgaagccga tgatcaggac cgtcttgccg
     6721 aacaaatcat aaggcagcat gcccagccgg tgcgtccatt tgccgtcctt gacaatggca
     6781 tgcatttcca ccgcgcgctt ggccagcatc agcatcatga acagcgcctg ttccgcgacc
     6841 gacggtgaat tggcggtacc cgccaccatc agcgggatgt tgcggcggct cagcgcggga
     6901 acatcgacgg cgtcgtagcc gacgccgatc cgggtcacca ccttcatgtc cttggacgcc
     6961 tcgagctccg gctcgccgaa acgggtgccg ccgagcgcca cgccgtgaac cggcgcctgc
     7021 gccctgagca tggactcgaa atcatgagcg gaaatcatat tgggaaattc gacgatttcg
     7081 atgtcgtccc gttcttttaa cagggctcgt ccctgctgcg acatcgattc agtgatgaaa
     7141 attttcttct tgttggtcgc cattccattc ccttgcccag ccgtttggct ttcttgttgt
     7201 gcgtccgtca aatgacggcg ccggtaacct catttagcag gtgcatattg ttgaggtcca
     7261 ctgccaatcg aagcggcgcg ccatcgcggg cgccggcgtt gggattgacc cgcccgcaga
     7321 tctgcgcgcc ttcgagcgtg aaataaatca acgtctccat gcccatcggt tcggtgacat
     7381 ccaacaccgc gtcaaaggcc tcgattccgg gccccgggtg cgccttcgcc tccgtgatat
     7441 gctctggccg caggcccagc agcaacttct cgtttcgagt gacgccctga tatctcgccg
     7501 cgcgattggc cggcaatgga aaggcgatgc ggtcggtcag ccggatgtgc agcttcccgg
     7561 cgacgtcctc gagcccgcac agcatgaaat tcatcgccgg cgagccgatg aagcctgcga
     7621 cgaagcgggt cgccgggttg tgatagagat cgttcggcgt tccgatctgt tcgatgcggc
     7681 cgtggttcat caccacgacc ctgtcggcga gcgtcatcgc ctcgacctgg tcgtgggtca
     7741 cgtagacggt cgtggtgcgg acc
//
LOCUS       DASWGM010000660         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1168734,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000660 DASWGM010000000
VERSION     DASWGM010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168734"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VGA15_18550"
     CDS             complement(<1..116)
                     /locus_tag="VGA15_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX9589731.1"
                     /translation="MSVPQTTNANDITSVIANLASRQGNRPKGPQMSGPKRAA"
     gene            complement(123..1742)
                     /gene="fliF"
                     /locus_tag="VGA15_18555"
     CDS             complement(123..1742)
                     /gene="fliF"
                     /locus_tag="VGA15_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX9589732.1"
                     /translation="MQGLAGFLKGLGASRLMAMVAVTAALIGFFAFVIMRVTTPQMTT
                     LFTDLSVEDSAGIVKDLERQAIPFELRNDGAVIMVPKDKVTRLRMKLAEGGLPKGGGV
                     GYEIFDKSDALGTTSFVQNINHLRALEGELARTIRAIDRVQAARVHLVLPERPLFSRE
                     TPEPSASIVVRVRGSLEPQQIRAVRHVVASAVSGLKPQRVSIVDEAGQLLADGATGDA
                     DNTIADERRSGFEKRMRSQVEAIVSSVVGAGRARVQLSADFDYNKITQTSDRFDPEGR
                     VLRSSQTREESSATAESNAQVTVNNELPGNQNRDNATPARDQSKKTEETNNYEISRTT
                     KTEVTEAGRVNRISVAVLVDGSYAKNDKGEMVYQERSKEQLDRIAALVRSAIGFDQKR
                     GDQVEVVNLKFAEAPLVVPVAEPTGLLGMLQFTKDDVMYVIELGVMMLLGLVVLFMVI
                     RPLVKRILASEEISSLIGDASVPALTDASAQPAGASQSLIPNATAQMIDVAQVQGQVH
                     AQSVHRVGELAERNPNETASIVRQWLTEPAT"
     gene            complement(1920..2195)
                     /locus_tag="VGA15_18560"
     CDS             complement(1920..2195)
                     /locus_tag="VGA15_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEX9589733.1"
                     /translation="MTETHRPRVKYVIGPDGSPLTIADLPAPGTKRWVIRRKAEVVAA
                     VRGGLLSLEEACSRYTLTVDEFLSWQFSIDQHGLAGLRTTRIQQYRQ"
BASE COUNT          422 a          685 c          740 g          454 t
ORIGIN      
        1 gcggcgcgtt tcggaccgct catctgcggg cctttcgggc ggttgccctg acggctcgcc
       61 aggttggcga tgacgctggt gatatcgttg gcgtttgtgg tttgcggtac ggacatgtca
      121 gatcacgtgg ccggctcggt gagccattga cgaacaatcg aagcggtctc gttggggttt
      181 cgctcggcca gttcgccgac gcgatgcacc gactgcgcgt ggacctggcc ctgaacctgg
      241 gcgacgtcga tcatctgcgc agtggcattg gggataaggc tttggctggc gcccgccggt
      301 tgtgcgctgg cgtcggtcaa cgccggcacg gaagcgtcgc cgatgagcga ggagatctcc
      361 tcggaagcga ggatgcgctt gaccagcggc cggatcacca tgaacaacac cacgaggccg
      421 agcagcatca tgacgccaag ctcgatgacg tacatgacgt catccttggt gaactgcagc
      481 atgccgagca ggccggtcgg ctcggcgacc gggacgacaa gcggcgcttc ggcgaatttg
      541 aggttcacga cctcgacctg atcgccgcgc ttctggtcga agccgatcgc cgagcgcacc
      601 agcgccgcga tgcggtccag ctgttccttg ctgcgctcct gatagaccat ctcgcctttg
      661 tcgttcttgg catagctgcc gtcgaccagc acggcgaccg aaatgcggtt gacccgtccg
      721 gcctcggtca cttcggtctt ggtagtgcgg gaaatctcgt aattgttggt ttcctcggtt
      781 ttcttgctct ggtcgcgcgc cggcgtggca ttgtccctgt tctgattgcc gggcaactcg
      841 ttgttgacgg tgacctgagc attgctctcg gcggtcgctg aagattcctc ccgggtctgg
      901 ctcgagcgca gcacccggcc ttcgggatcg aagcggtccg aggtctgggt gattttgttg
      961 tagtcgaagt cggccgagag ctggacgcgg gcgcggccgg cgccgaccac cgacgacacg
     1021 atcgcctcga cctgactgcg catccgcttc tcgaagccgg agcggcgctc gtcggcgatg
     1081 gtgttatcgg cgtcgccggt ggcgccgtcg gcgagcaatt gaccggcttc gtcgacgatc
     1141 gacacccgct gcggcttcag gccgctgacg gcggaagcga ccacgtggcg gacggcgcga
     1201 atctgctgcg gttcgagcga gccgcgcacc cgcaccacga tcgaggccga tggttcgggc
     1261 gtctcgcgcg aaaacagcgg ccgctcgggc aataccagat ggacccgcgc ggcctggacg
     1321 cggtcgatgg cgcggatggt acgggcaagt tcgccctcga gcgcgcgcaa atgattgatg
     1381 ttctggacga agctggtggt gccgagcgcg tcggatttgt cgaagatttc gtaaccgacg
     1441 ccgccgccct tgggcagccc gccctcggca agcttcatgc gcagccgcgt gaccttgtcc
     1501 ttcggcacca tgatcacggc gccatcgttg cgcagctcga acgggatcgc ctggcgttcc
     1561 aggtctttga cgatgccggc ggaatcctcg acgctgagat cggtaaagag ggtggtcatc
     1621 tgcggtgtgg tgacgcgcat gatgacaaag gcgaagaagc cgatcagcgc ggctgttacc
     1681 gcgaccatcg ccatcaaccg cgacgcaccc agacctttta ggaagcctgc aagaccctgc
     1741 aaaaccgacc gcccctcaga ttcggcccca aagcgccgac tgggcaatta ttgcctatgg
     1801 gatggtttcg atatggttaa cgaaggttaa gggctggggc gaaagttcaa catgaaaaaa
     1861 atcggcttcg caaaacgaag ccgattttca tcaaaatagt atgggttctg gaggggtgct
     1921 tattggcgat attgctggat gcgggtggtg cgcagtccgg caaggccatg ctgatcgatg
     1981 gaaaactgcc aggaaaggaa ctcgtcgacg gtcagcgtgt agcggctgca agcctcctcg
     2041 agggagagca ggccgccgcg cacagcggca acgacttcgg ccttccggcg gataacccac
     2101 cgtttggtcc caggcgcggg cagatccgca attgttaacg gactaccgtc aggcccgatg
     2161 acatatttta ccctcgggcg atgggtttct gtcatggcgt actcacaaac tctcaaccac
     2221 taactcacgc aacaacctac gcccggcgac ttaaaatttg cctaagccca aggcttcaat
     2281 aagaatctcc ttgaaaatgc c
//
LOCUS       DASWGM010000661         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2302499,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000661 DASWGM010000000
VERSION     DASWGM010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302499"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGA15_18565"
     CDS             <1..289
                     /locus_tag="VGA15_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589734.1"
                     /translation="AGVAAVVMLARFNSANAAYGESYLLVTILAAVLGGVDPFGGFGK
                     VGGLMLALIILQVISSAFNLLNLSQFLTLAIWGGILIAVAAVPHLRGKAER"
     gene            296..1717
                     /locus_tag="VGA15_18570"
     CDS             296..1717
                     /locus_tag="VGA15_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX9589735.1"
                     /translation="MTRSTEPTPIAVLDIGKTNLKLLVASDDGWPLETLSIPNAANAS
                     GPYPSYDLAGLEAWFLDALAEVSQRHAIGAVIATAHGCGAVLVDGDRPVLPMMDYDAV
                     SPPAIDEAYRLIAPAYREVFCGIGAGAMRLAKQLLWQESAFPAEFARAKTYLTTAQFF
                     AMRLGGRAASEISQLAAQGHIWDLIRHQPSSVMRERGWAHLPPQRAPAGAVLGTVSEA
                     VAKRTGLARSTEILCGVHDSNANLFRYKAAGMADASILSTGTWMIGFQRDFEPDKLDA
                     TRAMVLNIDVDGENVPSTLTMTGREYDLIRKERSAADAAVLAALPKLVARGSLALPSF
                     VGDDGLFPGAAHRGRIIGPAPETPAEWQGLAVLYAAFSANRCLDTLGSSKRIIIDGGF
                     AANLPFARALAALRPSQDVSVSQSRDGTALGAALLWRRFARRLPVSSVVLEAITPLGE
                     ASLDRPGLSAAYRSWIAFSEQVP"
     gene            1714..>1824
                     /locus_tag="VGA15_18575"
     CDS             1714..>1824
                     /locus_tag="VGA15_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HEX9589736.1"
                     /translation="MTDRNHADLRQGIVDTCQEMNRNGLNQGTSGNLSHRI"
BASE COUNT          302 a          599 c          558 g          365 t
ORIGIN      
        1 cgccggtgtc gcagccgtgg tcatgctggc acggttcaat tcggccaacg cggcctatgg
       61 tgagagctat ctgctcgtca ccatcctcgc ggcggttctc ggcggcgtcg atcccttcgg
      121 cggattcggc aaagtcggcg gactcatgct ggcgctgatc atcctgcagg tgatctcgtc
      181 ggcattcaac ctgctcaatc tcagccagtt tctcacgctg gcgatttggg gtggaatcct
      241 gattgccgtc gccgctgttc ctcacctgcg cggcaaagca gagcggtgaa cagcgatgac
      301 gcggtcgaca gaacccactc cgatcgccgt tctcgacatt ggcaagacca atctgaaact
      361 gctggtggcg agtgacgatg gctggccgct cgaaacgctt tcgattccga acgccgcgaa
      421 cgcttcaggc ccttaccctt cgtatgatct tgccgggctg gaggcgtggt ttctcgatgc
      481 gctcgccgag gtgtcgcaac gccatgcgat cggcgcggtg atcgcgacgg cgcatggctg
      541 cggcgccgtt cttgtcgatg gggacaggcc cgtcctgccg atgatggact atgatgcggt
      601 ctctccgccc gcgatcgacg aagcctatcg gctgatcgcg cccgcgtatc gcgaagtgtt
      661 ttgcgggatc ggcgccggcg ccatgcgcct cgccaagcag ctgctctggc aggagagcgc
      721 gttccccgcc gaatttgccc gcgccaaaac ctatctgacc accgcgcaat ttttcgccat
      781 gcggctcggc ggtcgtgcgg ccagtgaaat ctcgcagctt gcagcccagg gccatatctg
      841 ggacctgatc cgccatcagc cttcgtcggt catgcgcgaa cgcggctggg cccatcttcc
      901 gccgcaacgc gcccccgccg gcgctgtgct ggggacggtt tcggaagctg tggccaaacg
      961 caccggcctt gcgcgatcga ccgaaatcct gtgtggcgtg catgattcca atgcgaacct
     1021 gttccgttac aaggccgccg gcatggccga cgcgtcgatc ctgtcgaccg gcacctggat
     1081 gatcggcttt cagcgcgact tcgagccgga caagctcgac gccacccgcg cgatggtgct
     1141 aaatatcgac gtcgatggtg agaatgtgcc ttccacactg accatgaccg gccgcgaata
     1201 cgacctgatc cgcaaagaaa gaagtgctgc cgacgccgct gtccttgccg cgttgccgaa
     1261 actcgtcgcg cgggggagcc tggcgctgcc ctccttcgtt ggagatgatg ggctgtttcc
     1321 cggtgccgct catcgcggac ggatcatcgg tccagcgccc gaaacgcctg ccgaatggca
     1381 ggggctggcg gtgctgtacg cggcattcag cgcaaaccgc tgccttgata cgcttggcag
     1441 ttcgaaacgc atcattatcg atggcggctt tgccgccaac ctgcccttcg cgcgcgctct
     1501 tgccgccttg cggccatccc aagacgtctc ggtgagccaa tctcgcgacg ggacggcgct
     1561 cggcgcggcg ttgctctggc ggagatttgc gcgcaggctt ccggtttcga gcgtggtgct
     1621 tgaagccatc accccgctcg gcgaagcttc gctcgatcgc cccggtcttt ccgcagccta
     1681 tcgatcctgg atcgcctttt cggagcaagt accatgacag accgcaacca tgctgatctt
     1741 cgccagggca tcgtcgacac ctgccaggag atgaaccgaa acggcctcaa ccagggcacg
     1801 tcaggcaatc tctcgcaccg gatt
//
LOCUS       DASWGM010000662         6588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2021392,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000662 DASWGM010000000
VERSION     DASWGM010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021392"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGA15_18580"
     CDS             complement(<1..525)
                     /locus_tag="VGA15_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX9589737.1"
                     /translation="MTTFEWLIALLLGAVLLSALARRIKVPYPTFLAIGGMLLAFVPS
                     GPSWTLEPDLALALFVAPVLLDAAFDTSLRDLRDNWLPVSTLVFIAVGVTTAAVAALA
                     HWLLPDMPWAAAIALGAIVAPPDAAAATAILRQVNLPYRILKILEGESLLNDASALLI
                     YRVAVGAAAVEHFRW"
     gene            690..1214
                     /locus_tag="VGA15_18585"
     CDS             690..1214
                     /locus_tag="VGA15_18585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589738.1"
                     /translation="MRDGLSGLIRSVALASVLWATPAYHACATGTEPATDPQADPAPC
                     ITAAAAGDADKIIAVCGALVDNDKTLKPDRIKALIARAGAYNRRDMVDRAIGDYDTAL
                     RLDPTLADIFNARGELWHRKGDRPRALADFGAAIKLNPQHVTARANYRSLALELERIG
                     AQMAIKNRPIPPLK"
     gene            1335..3245
                     /locus_tag="VGA15_18590"
     CDS             1335..3245
                     /locus_tag="VGA15_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEX9589739.1"
                     /translation="MNIQDNSRYHEVHARSLADPEGFWGEAARAIDWIEPAKKVFDPA
                     SGLYGRWFAGAVVNTCYNALDRHVANGRADQVALIHDSPLAGSVTRLTYAQMLHEVKT
                     LAAIMGDFGVAKGDRVILYMPMVPEAMIAMLACARIGAVHSVVFGGFAAKELATRIED
                     AKPKLIFSASCGLEPGRIVQYKPLLDEAIKLSSDKPQACVILQRPQQSCELVSGRDHD
                     WAELRAKAMSAKKSAACVPVLATDPLYILYTSGTTGKPKGVVRDNGGHLVALKWSMFN
                     LYGVNPGEVWWCGSDIGWVVGHSYIIYGPLFHGATSIMYEGKPIGTPDAGAFWRVISE
                     HKAVALFTAPTAFRAIRKEDPDGKFIRQYDLSKFRTLFLAGERADPPTVEWAEQQLKV
                     PVIDHWWQTETGWCIAGNPVGLGMLPVKHGSPTVPMPGYQVDVVDEATKPLSAGTMGS
                     IVIKLPMPPGCLPTLWEQDERCRQAYFKEFPGYYKTSDAGYKDEDGYVYVMGRTDDII
                     NVAGHRLSTGGMEEILASHPDVAECAVLGIKDPIKGEVPCGFLVLKAGVTRQPGEIEK
                     EIVALVREKLGPVAAFKLAITVGRLPKTRSGKILRGTIKKIADGEEWVMPATIEDPKV
                     LDEIGDALKGRV"
     gene            3524..3793
                     /locus_tag="VGA15_18595"
     CDS             3524..3793
                     /locus_tag="VGA15_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589740.1"
                     /translation="MRRNSARLYIALLLIAPLLGGCLERGQPTMVNAGGDDDDAFCRA
                     SNAVGSPEYVACRKDRDVQRNNANAKADRAQRNLGEYMMNHPDHP"
     gene            3809..4015
                     /locus_tag="VGA15_18600"
     CDS             3809..4015
                     /locus_tag="VGA15_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX9589741.1"
                     /translation="MNEDVFNASLRKFLKKVGITSQREIEKAVREAVEAGRLKGHEKL
                     PAKMVLTVGGLSLTHEVTDEIELG"
     gene            4245..4463
                     /locus_tag="VGA15_18605"
     CDS             4245..4463
                     /locus_tag="VGA15_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEX9589742.1"
                     /translation="MDIKVRDSNGALLAEGDSVTLIKDLKLKGSSTVLKRGTMIKGIH
                     LTDNADEIEGRTDKVKGLVLRTEFLKKA"
     gene            4544..4963
                     /locus_tag="VGA15_18610"
     CDS             4544..4963
                     /locus_tag="VGA15_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEX9589743.1"
                     /translation="MSVEIEFPLEFLVAGTPVSLQAKRRESVDQWKARIIDASRSILP
                     EGHFATENPIAATLFYFPAAEMAGDIDNIVKPVLDALGNHIYVDDRQVHRVLVQKFEP
                     GNVFGFEAPSPTLQDALNRAKPILYVRLSNDPFEDLA"
     gene            4963..5613
                     /locus_tag="VGA15_18615"
     CDS             4963..5613
                     /locus_tag="VGA15_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589744.1"
                     /translation="MSVFGSSEAEVIESLLPRYQAEGFDVYVNPSPSILPPFMQTYRP
                     DAIALKKDKKIAIEVVGSTTRSEQKIKTLQSLFADHDDWELRVFYASPIASNKLLGIA
                     SIPAINESIQRVIDLKEAGHLLPALVMAWATFEAVGRALLPEQFQRPQTPARLVEVLG
                     SQGYITPEEADTLRAASTIRNAVVHGQLDSTVDQKMLEGFVAILKTLVKLLPAGNS"
     gene            5649..5801
                     /locus_tag="VGA15_18620"
                     /pseudo
     CDS             5649..5801
                     /locus_tag="VGA15_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
BASE COUNT         1377 a         1966 c         2073 g         1172 t
ORIGIN      
        1 ccacctgaaa tgctccaccg cggcggcgcc gacggcgacg cgatagatca gcagcgcgct
       61 ggcgtcgttg agcaggcttt cgccttcgag gatcttgagg atccgatagg gcaggttgac
      121 ctggcgcagg atcgccgtcg ccgcggcagc atctggcggc gccacgatgg cgccgagggc
      181 gatcgccgct gcccatggca tgtccggcag caaccaatgg gcgagcgccg ccaccgcggc
      241 ggtggtcacg ccgaccgcga tgaataccag cgtcgagacc ggcagccagt tgtcccggag
      301 gtcgcgcagc gaggtgtcga aggcggcgtc cagtaaaacc ggcgccacga acagcgccag
      361 cgccagatcg ggctccagcg tccaggacgg gccggatggc acgaaggcca gcagcatgcc
      421 gccgatggcg agaaaggtcg ggtagggcac cttgatccgc cgggccagcg ccgacagcag
      481 caccgcgccc agcagcaagg cgatcagcca ttcgaatgtc gtcaaacctg gggtccttgt
      541 cggcgggtct tgtcggtcag ccgccggcaa tctagcaccg aaatgcgccc ggtttaatcg
      601 cccgaaatcc gccgttgtcc gaaccgcctg ccgcttgcta cggtcgttgc aatagcgaaa
      661 tatcgaacag cggtacgcgg gacagattga tgcgagatgg tttgtcaggc ttgatccgga
      721 gcgtggcgct cgcgtctgtc ctgtgggcaa caccggcgta ccacgcctgc gcaaccggca
      781 ctgagcccgc cacggatccg caagccgacc ccgcgccgtg catcaccgcg gcggcggccg
      841 gcgacgccga caagatcatt gccgtctgcg gcgcgctggt cgacaacgac aagacgctaa
      901 agcccgaccg catcaaggcg ctgatcgcgc gcgccggcgc ctataaccgc agggacatgg
      961 tcgatcgtgc catcggcgac tacgacacgg cgctgcggct cgatccgacg ctggcggata
     1021 tcttcaacgc ccgcggtgaa ctctggcacc ggaagggcga ccggccccgc gcgctcgccg
     1081 atttcggtgc ggccatcaag ctcaatccgc agcacgtgac ggcgcgcgcc aactacaggt
     1141 cgctggcact ggagctggaa cgcatcggtg cgcagatggc gatcaagaac aggcctattc
     1201 cacccttgaa ataaggctgc ttcgcgtgac aatggcagcc acaaaaatac ggcccatttg
     1261 atcctggtca tggcggggcg cgccccggcc attcacgtca cgatctcaat aatgagttga
     1321 gcgggagcaa cgccatgaac atccaggaca acagccggta tcatgaggtt catgcccgct
     1381 cgctggctga tccggagggc ttttggggcg aggcggcgcg cgcgatcgac tggatcgagc
     1441 cggcgaaaaa ggtgttcgat ccggcaagcg gcctctatgg ccgctggttc gcgggcgccg
     1501 tcgtcaacac ctgctacaac gcgctcgacc gtcatgtcgc caatggccgc gccgatcagg
     1561 tggcgctgat ccacgattcg ccgctggcgg gcagcgtcac cagattgacc tacgcgcaga
     1621 tgctgcatga ggtgaaaacg ctcgccgcca tcatgggtga ttttggcgtc gccaagggcg
     1681 accgcgttat cctctatatg ccgatggtgc cggaagccat gatcgcgatg ctggcctgcg
     1741 cgcggatcgg cgcggtgcac agcgtggtgt tcggcggttt tgcggcgaaa gaactcgcca
     1801 cccggatcga ggacgcaaaa ccaaaactga ttttttccgc aagctgcggg ctcgagcccg
     1861 gccgcatcgt gcaatacaag ccgctgctcg acgaggccat aaagctttca agcgacaagc
     1921 cgcaggcctg cgtcatcctg cagcggccgc agcaatcatg cgaactcgtc agcggccgcg
     1981 atcacgactg ggcggagctg cgtgccaagg cgatgtcggc gaaaaaatcc gccgcctgcg
     2041 tgccggtgct cgcgaccgat ccgctctata tcctctacac ctcgggcacc acgggtaaac
     2101 ccaaaggcgt ggtgcgcgac aatggcgggc acctcgtcgc attgaaatgg tcgatgttca
     2161 atctctacgg cgtcaacccc ggcgaggtct ggtggtgcgg ctccgacatc ggctgggtgg
     2221 tcggccacag ctacatcatc tatgggccgc tgtttcatgg cgcgacctcg atcatgtatg
     2281 agggcaagcc gatcggcacg ccggatgccg gcgcgttctg gcgggtgata tccgagcaca
     2341 aggcggtggc gctgttcacc gcgccgaccg cgttccgcgc catccgcaag gaagaccccg
     2401 acggcaaatt catccgccag tatgatctgt caaaattccg cacgctgttt ctcgccgggg
     2461 agcgcgccga tccgccgacg gtggaatggg cggagcagca actgaaggtg ccggtaatcg
     2521 accattggtg gcagaccgaa accggctggt gcatcgccgg caatccggtg gggctgggca
     2581 tgctgccggt caagcacggc tcgccgacgg tgccgatgcc gggctaccag gtcgacgtgg
     2641 tcgatgaagc cacaaagcct ctgtccgcgg gcacgatggg ctcgatcgtc atcaaactgc
     2701 cgatgccgcc gggctgcctg ccgaccttgt gggagcagga cgagcgctgc aggcaagcct
     2761 acttcaaaga gtttcccggc tactacaaga cctccgacgc cggctacaag gacgaggacg
     2821 gctacgtcta tgtgatgggc cgcaccgacg acatcatcaa cgtcgccggc caccggctct
     2881 ccaccggcgg catggaggag attctggcca gccatcccga cgtcgccgaa tgcgcggtgc
     2941 tcggcatcaa ggatccgatc aagggcgaag tgccctgcgg ctttctggtg ctgaaggccg
     3001 gcgtgacgcg tcagcctggc gagatcgaaa aggagatcgt ggcgctggtg cgcgaaaagc
     3061 tcggcccggt ggcggcgttc aagctcgcga tcaccgtggg ccggctgccg aaaacgcgct
     3121 cgggaaaaat cctgcgcggc accatcaaga agatcgccga cggcgaggaa tgggtgatgc
     3181 cggcgacaat agaggatccg aaggtgcttg acgaaatcgg cgacgcgctg aagggaaggg
     3241 tgtgaatcgc gacagtcgtc attccggggc gtgcgaagca cgaacccgga atctcgagat
     3301 tccgggttcg atgcggagcc tgtcatcggg ccgcacttcg cgcggacccg ttggcatcgc
     3361 cccggaatga cgatttctcg tcatgcccgg gcttgacccg ggcatccatc acctgccaag
     3421 caactgcctt gttttcgatg gattgccggg tcactcagcg aagacgcgct tggcgctttt
     3481 gcccggcaat gacgcgcact ggaacaacga acggaacccg cgcatgcgac gtaactccgc
     3541 acgactctat atcgcgctat tgctgatcgc gccgttgctc gggggctgcc tcgagcgggg
     3601 acagccgacc atggtcaatg ccggcggcga tgacgacgat gcattctgcc gcgccagcaa
     3661 tgcggtgggc tcgcccgaat atgtcgcctg ccgcaaggac cgcgacgttc aacgcaacaa
     3721 cgccaatgcg aaggccgacc gggcgcagcg caatctcggc gaatacatga tgaaccatcc
     3781 ggaccatccg taacatcagg aggccgtcat gaacgaagac gtcttcaatg ccagcctgcg
     3841 caaattcctc aagaaggtcg gcatcacctc gcagcgcgaa atcgaaaagg cggtgcgcga
     3901 ggcggtggag gccggccgtc tcaagggcca tgaaaaactg ccggccaaaa tggttctgac
     3961 cgtgggcggc ctcagcctca cgcatgaggt caccgacgaa atcgaactgg gttagcgcaa
     4021 cgcggcgctc gttaaatccc tcccccttgt ggggagggtc ggccgagcga agcggaggcc
     4081 ggggtggggg tagcgcacgg cagtgcgcga acaatcactt tgcggaagcc gccccccacc
     4141 cgaccggcct tcggccggcc acctaagagc gagcttcgct cgtctcgacc ccacgagggg
     4201 gagggaaaag agacgcagcc tttcatcaca aaggacaata ccgcatggac atcaaggtca
     4261 gggattccaa cggcgcgctg ctcgccgagg gcgacagcgt cacgctgatc aaggatctga
     4321 agctgaaggg ctcctccacg gtgctcaagc gcggcactat gatcaaaggc attcacctca
     4381 ccgacaacgc cgacgagatc gagggccgca ccgacaaggt caagggcttg gtgctgcgca
     4441 cggagttctt gaagaaggcg tagcagacag agttgaaacg gtagcttgct catgtaagcc
     4501 tgcacccatt gtgcgacgat attttggccc gcaaggctta gtaatgagcg tggaaatcga
     4561 atttcctctc gaatttttgg tggccggaac accggtttcg cttcaggcca agcgccgtga
     4621 atcggtggac cagtggaagg cccgcatcat tgacgcaagt cgatcaatct tgccggaggg
     4681 ccattttgca acggaaaacc caatagcggc aaccttgttt tatttccctg cggccgaaat
     4741 ggcgggcgac atcgataaca tagtgaaacc tgtgcttgat gccttgggca accacattta
     4801 tgtggatgat cgacaggtac accgggtctt ggttcagaaa ttcgagcctg gaaacgtttt
     4861 cgggttcgaa gcgcctagcc caacactgca ggatgctcta aatagggcta agccgatctt
     4921 atatgttcgg ttatcgaacg atccattcga ggatctcgca taatgagcgt ttttggaagc
     4981 agtgaagcgg aagttattga gagtctcctg ccgcgctatc aagcggaagg tttcgacgta
     5041 tatgtaaacc cgtccccctc aatccttccg ccttttatgc agacttacag accggacgcc
     5101 atcgccctta agaaagacaa gaaaatcgca attgaagtag tcgggtcaac cacccgctca
     5161 gaacagaaaa taaaaacgct gcagtcgtta tttgcggacc acgatgattg ggaactccgc
     5221 gtcttctacg cttcaccgat cgcatccaac aagttgttgg gaatagcttc aattcctgca
     5281 atcaatgaat ctattcagcg ggtcattgat ttgaaggagg cggggcattt gttgcccgct
     5341 ctcgtgatgg cgtgggccac atttgaagcc gttgggcgag ctttgcttcc cgagcaattt
     5401 cagcggcctc aaacccctgc tcgcctcgtc gaggtgcttg ggtcgcaagg gtatattacg
     5461 cccgaggaag ccgatacatt aagagccgct agtacaatcc gaaatgcggt ggttcatggt
     5521 caacttgatt ccactgtcga tcaaaagatg ttggaaggtt tcgttgctat tctaaagacc
     5581 ttagtcaagt tgctgcctgc gggaaattcg taacagaatc cggcgcgtgg cgcggttcaa
     5641 catggcctga ggatctgaag ctgaagggct cctccacggt gctcaagcgc ggcactatga
     5701 tcaaaggcat tcacctcacc gacaacgccg acgagatcga aggccgcacc gacaaggtca
     5761 agggcctggt gctgcgcacg gagttcttga agaaggcgtg agagaaacgt ggcaatgtgc
     5821 cttcttgacc tcgccccgct ctttgcgggg agaggtcggc gcgtagcgcc gggtgagggg
     5881 ccgggcaggg cgcccgctag aataacaact ttgcgggtcg cagagtcgtc gaactaaacg
     5941 tgaaacagga ttgttggcgt gactgatgtg acccgagagt catacgatgg tggcctcgca
     6001 ttgcctggcc cctcacccgg attgcttcgc aatccgacct ctccccgcaa agagcggggc
     6061 gaggttagga aaagtcgccc gcaatcgacc gcctgctact gtcaatgcag gtcccgggtc
     6121 tgcggagcag cgtgaaggac actgcaccgc gtccgggata cgagcggaat atgctctcgc
     6181 tgccgtatgg tgatttgccc gtcgggcggt ttgctgacgg gggtgtccag ccccctttgc
     6241 aaaaatattt ctgttttcgc accccccaaa tcacgtctag aactttgcgc atcccgtccc
     6301 acacagaggg gcgtttcgcg atcgtcacgg acgttggcgg cgggatgcgg tggacgcggg
     6361 cagcgctgct gacgagagcg cttttcgcgg acggcgaagt cgtgtggtcc tgacgccccg
     6421 acgctggtgt caaggtggtg gatgaaaatc cgctgacgac ggtggcaaac aagcccggtc
     6481 accggggaga gcacgaagga aacgtgtgcc gtggaaaggc acggttcttg agagggtgca
     6541 agtccctccc ggcaagggtc gctccagccg gtagcaatcg gagcaggc
//
LOCUS       DASWGM010000663        10487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2021864,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000663 DASWGM010000000
VERSION     DASWGM010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021864"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..639
                     /locus_tag="VGA15_18625"
     CDS             43..639
                     /locus_tag="VGA15_18625"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX9589745.1"
                     /translation="MTFTLPTLPYAYDALAPHMSKETLEYHHDKHHQAYVTNGNNLLK
                     GTEFEGKSLEEIVKGSFGKNAPLFNNAGQHYNHLHFWSWMKPNGGGDKLPGRLEKKIT
                     EDLGGLEKFKTDFAAAGVGQFGSGWAWLSVKNGKLEISKTPNGENPLVHGATPILGVD
                     VWEHSYYIDYRNRRPDYLKAFVDHLVNWEYVDQLFSKA"
     gene            877..1476
                     /locus_tag="VGA15_18630"
     CDS             877..1476
                     /locus_tag="VGA15_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX9589746.1"
                     /translation="MSELPLKDPAPADDAAEAEPRPGHIRKPIGWSMIVIAALVAVSA
                     TLVWRRDGIDGVLEILTHDLSLFGGILPRVLAGCLLGAFITEILPHEKVSRSLGPNSG
                     LKGLLIGTAFGAILPGGPFTAYPVASALLTVGADFGATIAMVVSWTLIGYGRAVAWEL
                     PIMGTDFTLWRIVISLPLPVLAGAFGRFVYVRMYPKVAP"
     gene            1473..1970
                     /locus_tag="VGA15_18635"
     CDS             1473..1970
                     /locus_tag="VGA15_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019200036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589747.1"
                     /translation="MSALIIDIILWASVASLGFIAFRRGRPVLTASLREGSMDFINIV
                     PRIALGVIGSGYIAAVIPQEIITGRLGPNSGWLGVLTAVIAGAATPGGPVVGFSLGAV
                     ALKAGGGAPQVIAYVIAWALFAFQRVLLWEIPFMPARFVWFRAAVSVPFPFLAAAIAM
                     AIGKP"
     gene            2026..2253
                     /locus_tag="VGA15_18640"
     CDS             2026..2253
                     /locus_tag="VGA15_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589748.1"
                     /translation="MTRLLEQAIETVATLPDDTQDELARILLQLAGVEQPPYELTPEE
                     EADIDASLAEAERGEFATDEEVRAMWAKHGL"
     gene            2250..2525
                     /locus_tag="VGA15_18645"
     CDS             2250..2525
                     /locus_tag="VGA15_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9589749.1"
                     /translation="MKVRYTRCALRQMTEVLDYIEARSPSGAENVKRRLQGIIDLLAD
                     HPNGGRTINKGNLRRFVVTPYPYVIFYRADATGIVIHGVRHAARRSL"
     gene            2584..2772
                     /locus_tag="VGA15_18650"
     CDS             2584..2772
                     /locus_tag="VGA15_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589750.1"
                     /translation="MVPSHPHGHDHGHAHAHGHGPATPHPAQAAPWSILRMTVVLRLG
                     AALAVSAGLWAVVWLSMR"
     gene            2775..3533
                     /locus_tag="VGA15_18655"
     CDS             2775..3533
                     /locus_tag="VGA15_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9589751.1"
                     /translation="MAAQLQFRDVTLGYDRHPAVHHLNGEVAPGALLAIVGPNGAGKS
                     TLFRGIAGILKPLAGSIETGGLDIRDVAYLPQTVDIDRSFPISVFDLVGTGLWRRVGF
                     FGGMGKAARDKIAQALAAVGLNGFENRSIGTLSGGQMQRMLFARVLLQDARLIVLDEP
                     FNAIDAKTSADLLALVRRWHTEKRTVLTALHDLDLVRANFPETLLLARGMVAWGATAE
                     VLTAQNLLEARRMCEAFDDSAAACAVDDTPSRAA"
     gene            3539..4408
                     /locus_tag="VGA15_18660"
     CDS             3539..4408
                     /locus_tag="VGA15_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX9589752.1"
                     /translation="MIYDALIAPFTDFEFMRRALAAVIALSLGGAPIGVFLMLRRMSL
                     VGDAMAHAILPGVAIGFLLSGLNLFAMTAGGLIAGFAVAILAGVVARTTELKEDASLA
                     TFYLVSLALGVTIVSIKGTNIDLLHVLFGNILAMDDQTLLVIAFNATITLLVLAVIFR
                     PLVIECVDPVFLRTVSRAGAPAHLAFLALVVINLVNGFHALGTLLAVGLMILPAGIAR
                     FWSRDITAMICIAVASAMVSGYAGLVLSFQTKVPSGPAIILVAALLYVFSVLFGSVSG
                     LVRQMFPGRHLEA"
     gene            4627..5496
                     /locus_tag="VGA15_18665"
     CDS             4627..5496
                     /locus_tag="VGA15_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX9589753.1"
                     /translation="MRHLWLILLSFVAVISPARAQDRVNVVTSFSILGDFVKNVGGER
                     VSVTTLVGPDGDVHVYTPAPADAKKIADAKLLVINGLGLEGWLPRLLQASGSKAPIII
                     ATKNIAPLKLGSDADPHAWQSVANAKIYVTNIRDALGAADPADAATFRANTQAFLAKL
                     DALDREVREAIGKIPESRRKVISTHDAFGYFASAYGIEFVAPLGVSTESEASARDIAG
                     IITQIKTAKIPAVFLENISDPRLIRQIATETGARIGGTLYSDSLTGEKGDAPTYIDMV
                     RHNIRALTSALAN"
     gene            5548..6591
                     /locus_tag="VGA15_18670"
     CDS             5548..6591
                     /locus_tag="VGA15_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9589754.1"
                     /translation="MPELTSPKIPVTVLTGYLGAGKTTLLNRILSENHGKKYAVIVNE
                     FGEIGIDNDLIIGADEEVFEMNNGCVCCTVRGDLVRILDGLMKRKGKFDAIIVETTGL
                     ADPAPVAQTFFVDEDVQKNARLDAVVTVADAKWLNARLKDAPEAKNQIAFADVIVLNK
                     TDLVSKAELAEVEARIRAINPYAKLHRTERCQVALSDVLERGAFDLDRILELEPEFLD
                     AGDDHDHHDHDHHGHGHDHGHSHGLKHYHDEDMQSLSLRSDKPLDATKFMPWLQNLVA
                     SEGQKILRSKGILAFSDDDDRYVFQGVHMMLEGDHQRAWKDGEARESRLVFIGRELPE
                     QTIRDGFESCITT"
     gene            6591..7610
                     /locus_tag="VGA15_18675"
     CDS             6591..7610
                     /locus_tag="VGA15_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX9589755.1"
                     /translation="MKEFDPGDNAASIVSVTDRVRPVAIGMPVTSVHFLGERAAFVGA
                     EESVAMVSADGEILPVAVHGGGILSAASDGERIVMGGDDGKLVALDAKGEVALIATDP
                     KRRWIDNVALHPDGAVAWSAGKTAFVRGGKGEEKSFDVPSTVGGLAFAPKGLRLAVAH
                     YNGVTLWFPNMVATAEVLEWAGSHLGVVFSPDNKFLVTAMHEPALHGWRLADHRHMRM
                     TGYPGRVRSMSWSAGGKALATSGADTVILWPFASKDGPMGKEPAMLAPLQARVSMVAC
                     HPKQDIFAAGYSDGTILMVRLTDGAEILVRKPGAVPVAALAWNAKGTLLAFAAEDGDA
                     GLLEL"
     gene            7707..8306
                     /locus_tag="VGA15_18680"
     CDS             7707..8306
                     /locus_tag="VGA15_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX9589756.1"
                     /translation="MSTDAISLSNALFSKVQQRVLALIFGQPERSFYTSEIKRNVNSG
                     TGAVERELSRLQRSGLVSIERIGNQKHYRANRQSPIFAELHSLVIKTVALTEPLRKSL
                     EPYSDKIKAAFVYGSVAKGTDTARSDIDLLVIGDELSYSELYAALQNVENALGRKVSP
                     TFLSPKDWRRKASDRGSFISKVNALPKIFVFGSEKDLQL"
     gene            8303..8731
                     /locus_tag="VGA15_18685"
     CDS             8303..8731
                     /locus_tag="VGA15_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589757.1"
                     /translation="MSKEELDNLVRINRLKAEPGSRGEFAGMLNSARTRLTDAQNESL
                     DPDSQFDLAYGAAHRLALAALRHQGYRSENRITVFQTLVHTLGSDSADVQIFLKAHNE
                     RNLAEYEGRMEIDEGLLADLIRCTKKIETAVGKLDPPTGE"
     gene            8903..9202
                     /locus_tag="VGA15_18690"
     CDS             8903..9202
                     /locus_tag="VGA15_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589758.1"
                     /translation="MDLLVASAMVAAGLAVSGASLVLLNAETTQMAQATPPLQSTPGA
                     ETKPSAPAEPVTTGARPSQIPPEPARPDADAEKAGATPALPPAPAEKTAAPVKDR"
     gene            9411..10151
                     /locus_tag="VGA15_18695"
     CDS             9411..10151
                     /locus_tag="VGA15_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HEX9589759.1"
                     /translation="MKIDDVRRTAYSMPLTNPSFPPGPYRFFNREYFVITYRTDPEAL
                     AAVVPEPLEVTEPIVKYEFIRMPDSTGFGDYTETGQVIPVRLKGEEGVYTHAMYLDDD
                     APIAGGRELWGFPKKRATPKIEVESDVLVGSLHYGSVLCASATMGYKHRPIDHDTVLK
                     SMKAPNFILKIIPHVDGSPRICELVRFSLEDITLKEAWTGPAALGLFPHALADVARLP
                     VREVVSALHFKADLTLGLGKVAFDYLAK"
     gene            complement(10260..>10487)
                     /locus_tag="VGA15_18700"
     CDS             complement(10260..>10487)
                     /locus_tag="VGA15_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HEX9589760.1"
                     /translation="SAVLAGMVWALENPTAGIVEADEMDFSRCLEIQRSYLGPVKGFY
                     TDWTPLSDRPGLFPEDIDESDPWQFRNVLVR"
BASE COUNT         1959 a         3240 c         3238 g         2050 t
ORIGIN      
        1 ttcccgttaa accccgtctc ccaccagtgc ggacatcatc ccatgacctt cacgcttccc
       61 acccttcctt acgcctatga cgcgcttgcg ccccatatgt ccaaggaaac cttggaatac
      121 caccacgaca agcatcacca ggcctatgtg accaacggca acaatctgct gaaaggcacc
      181 gaattcgagg gcaagtctct tgaggagatc gtcaagggct cgttcggcaa gaatgcgccg
      241 ctgttcaaca acgccggcca gcattacaac catctgcatt tctggagctg gatgaagccc
      301 aatggcggcg gcgacaaatt gcccggccgc ctggaaaaga agatcaccga ggacctcggc
      361 gggctggaga aattcaagac cgatttcgcc gccgccggcg tcggccagtt cggttcgggc
      421 tgggcctggt tgtcggtgaa gaacggcaag cttgaaatct caaaaacccc caacggcgaa
      481 aacccgttgg tgcatggcgc cacgccgatc ctcggcgtcg acgtctggga gcactcctat
      541 tacatcgatt atcgcaatcg ccgcccggat tatctgaagg cgtttgtcga tcatctggtg
      601 aactgggagt atgtcgacca gctgttctcg aaagcctgac gccaccaggc gacaatctgc
      661 cagccaactg ttccaaaggg cggtagcatc tggctgccgc cctttcgttt gctctgccgt
      721 acgcgccggg gtcggcggtg tttgcttgct gccgtggcaa tcccgcatgg gcatccatga
      781 agcacgcatg ggtattgccg ttgttccgtt tgcttcgccg tgccggcgag actaagcagg
      841 tcgagccagc ttcatgaccc tccagacccc attgccttgt cagaactccc gctaaaagac
      901 ccggcgccgg ccgacgacgc cgcggaggcc gagccgcgtc ccggacacat ccgcaagccg
      961 atcggctggt cgatgatcgt gattgcggcg ctggtcgcgg tgagcgccac gctggtctgg
     1021 cggcgcgacg gcatcgacgg cgtgctggaa atcctgaccc acgacctctc gcttttcggc
     1081 ggcatcctgc cacgcgtgct ggcgggctgc ctgctcggcg cgttcatcac cgagatcctg
     1141 ccgcatgaga aagtgtcgcg atcgctgggg ccgaattccg ggctgaaggg acttttgatc
     1201 gggaccgcgt tcggcgcgat tctgccgggc gggccgttca ccgcctatcc ggtggcgagc
     1261 gcgctgctca cggtcggcgc cgatttcggc gccaccatcg ccatggtcgt gagctggacg
     1321 ctgatcggct acggccgcgc ggtcgcctgg gaattgccga tcatgggcac cgatttcacg
     1381 ctgtggcgca tcgtgatctc gctgccgctg ccggttctgg ccggcgcgtt cggtcgtttc
     1441 gtctatgtcc ggatgtatcc gaaggtcgcg ccgtgagcgc cctgatcatc gatatcatcc
     1501 tgtgggcctc ggtcgcctcc ctcggattta tcgctttccg gcgtggccgc cccgttctga
     1561 cggcctcatt gcgcgaaggc agcatggatt ttatcaatat cgtgccgcgc atcgcgctcg
     1621 gcgtgatcgg ttcgggctat atcgcagccg tcattcccca ggaaatcatc accggccggc
     1681 tcggacccaa ctcgggctgg ctcggcgtcc tcaccgccgt gatagcgggt gcggcgactc
     1741 cgggcgggcc ggtggtcggc ttttcgctcg gggcggtagc gctgaaggcc ggcggcggcg
     1801 cgccgcaggt gatcgcctat gtgatcgcct gggcgctgtt cgccttccag cgcgtgctgc
     1861 tgtgggaaat cccgttcatg ccggcgcggt ttgtctggtt ccgcgcggcg gtatcggtgc
     1921 cgttcccgtt cctggcggcg gcaatcgcga tggcgatcgg gaagccttag agccggtcga
     1981 ggtcggagcc ttcctaaggg gagctgaaag cactatattt cgaatatgac ccgattgctt
     2041 gaacaggcca ttgaaaccgt ggcgactttg cccgacgaca cgcaggacga acttgcgcgg
     2101 attctcttgc aactcgcggg agtagagcaa ccgccctatg aactcacgcc cgaagaagaa
     2161 gctgacatag acgcctcgct ggccgaagcc gagcgtggcg agttcgcgac ggacgaagaa
     2221 gtccgcgcca tgtgggcaaa gcatggcctg tgaaagtccg ctacacgcgc tgcgcgctcc
     2281 ggcagatgac cgaggttctg gactacattg aggcccggtc gccgagcggc gcggagaacg
     2341 tcaagcgacg gctacagggc attattgatc tactcgctga ccatccaaac ggcggacgca
     2401 ccatcaacaa gggcaatctc cgccgcttcg tggtaactcc gtacccttac gtaatcttct
     2461 accgagcgga cgcgaccggg atcgtcatcc acggcgttcg ccatgctgcc cgccgttccc
     2521 tttgaactta agcaagcggt ccggttggtg gactcaagtt atgttataat ataacatttc
     2581 ttcatggttc cttcccatcc ccacggccat gaccacggtc acgcccatgc ccacggccac
     2641 ggcccggcaa cgccgcatcc ggcgcaggcc gcaccctggt cgatcctgcg catgaccgtc
     2701 gtgttgcggc tcggtgcggc gctggcggtc agcgctggcc tgtgggcggt cgtgtggttg
     2761 tcgatgaggt gagcatggcc gcgcaactgc aattccgcga cgtcacgctg ggctacgacc
     2821 ggcatccggc ggtgcatcat ctcaatggcg aagtggctcc cggcgccttg ctcgcgatcg
     2881 tcggcccgaa cggcgccggc aaatcgacgc tgtttcgcgg catcgccggc atcctcaaac
     2941 cgctggcggg ttcgatcgaa accggcggtc tcgatatcag ggatgtcgcc tatctgccgc
     3001 agacggtgga tatcgaccgc agctttccga tctcggtgtt cgatctggtc ggcactggat
     3061 tgtggcgcag agtcggattt ttcggcggca tgggcaaggc cgcgcgcgac aagatcgcgc
     3121 aagcgctcgc cgccgtcggc ctcaacgggt ttgagaatcg cagcatcggc acgctgtccg
     3181 gcgggcagat gcagcgcatg ctgttcgcgc gggtgctgtt gcaggacgcg cgcctgatcg
     3241 tgctggatga gcccttcaac gccatcgacg ccaaaacctc ggccgatctg ctggcgctgg
     3301 tgcggcgctg gcacactgaa aaacgcaccg tgctgacggc gctgcatgac ctcgatctgg
     3361 tgcgcgccaa tttcccggaa accttgctgc tggcgcgagg catggtcgcc tggggcgcca
     3421 ccgccgaggt attgaccgcg caaaaccttc tggaggcgcg gcggatgtgc gaagcgttcg
     3481 acgacagcgc cgccgcctgc gcggtggacg atacgccttc gcgggccgcc tgagtgtcat
     3541 gatctatgat gcgctgatcg cgcccttcac cgatttcgaa ttcatgcgcc gggcgctggc
     3601 ggcggtgatc gcattgtcgc tcggcggcgc gccgatcggc gtgttcctga tgctgcggcg
     3661 catgagcctg gtcggcgacg ccatggcgca cgccatcctg ccgggcgtgg cgatcggttt
     3721 tttgctgtcc ggcctcaatc tcttcgcgat gacggcgggc ggattgatcg ccggctttgc
     3781 ggtggccatt ctcgcgggcg tggtcgcgcg gaccaccgag ctgaaggaag atgcctcgct
     3841 ggccacgttc tacctggtgt cgctggcgct cggcgtaacc atcgtgtcga tcaaaggcac
     3901 caatatcgat ctgctgcatg tgctgttcgg caatatcctg gcgatggacg accagaccct
     3961 gctggtgatc gccttcaacg ccacgatcac gcttctcgtg ctcgcggtga tttttcgccc
     4021 gctggtgatc gagtgtgtcg atccggtgtt cttgcgcacc gtcagccgtg ccggcgcgcc
     4081 ggcgcatctg gcgtttctgg cgctggtcgt tatcaacctc gtcaacggat ttcatgcgct
     4141 cggcactctc ttggcggtcg ggctgatgat attgcccgcg ggcatcgcgc ggttctggtc
     4201 gcgtgatatc accgccatga tctgcatcgc ggtcgcgagt gcgatggttt cgggctatgc
     4261 cgggctcgtg ctgtcgtttc aaacaaaagt gccgtcgggg cccgcgatca ttctggtggc
     4321 ggcgctgctg tatgttttct cggtgctgtt cgggagcgtc agcggcctgg tccggcagat
     4381 gtttcccggc cgccacctcg aagcgtagcg tgattcatgc atttggaatc gtcagactcg
     4441 atgccacctc tccccttgtg ggagaggtcg gatcgcatcg acagatgcga tccgggtgag
     4501 gggttacgct ccatcgatac gccgtgaccc ctcaccccaa ccctctccca caaggggaga
     4561 gggagcgcgc tgccgttgcc gggaaattga gccaatgctt caattcagat ggatcagact
     4621 ctagcgatgc ggcatctctg gctcatcctt ctgtcgttcg tggcggtcat ttcccccgcg
     4681 cgcgcgcaag atcgcgtgaa cgtggtcacg agcttttcca ttctcggtga tttcgtaaaa
     4741 aatgtcggcg gcgagcgcgt cagcgtgacg acattggtcg gccccgacgg cgacgttcac
     4801 gtctatacac cggcgccggc cgacgccaag aagatcgccg acgcaaaact ccttgtcatc
     4861 aacggtctcg gcctggaggg ctggctgccg cggctgctgc aagcctccgg cagcaaggcg
     4921 ccgatcatca tcgccaccaa aaacatcgcg ccgctcaaac tgggctccga tgccgatccg
     4981 catgcctggc aatcggtcgc caacgcgaag atatatgtga ccaatatccg cgacgcgctt
     5041 ggcgccgcgg acccggccga cgccgcgact tttcgggcca acacgcaagc ctttctggcg
     5101 aagctggatg cgctcgaccg agaggtacgc gaggccattg ggaaaatccc tgaaagccgc
     5161 cgcaaggtga tctccaccca tgacgcgttc ggctatttcg catcagccta tggcatcgaa
     5221 ttcgtagcac ccctgggcgt ttccaccgaa tccgaggcca gcgcgcgcga tatcgccggc
     5281 atcatcaccc agatcaaaac cgcaaaaatc ccggcggttt ttctcgaaaa tatcagcgat
     5341 ccacggctga tccgccaaat cgccaccgag accggcgcca ggatcggcgg aacgctctat
     5401 tccgacagtc tgacgggtga aaagggcgat gcccccactt acattgatat ggtcaggcac
     5461 aatataaggg cattgacgag cgcgctggca aattagggca ggggccctcc ctgctgccgt
     5521 gcgccgcata tcgtttccgg agtgatgatg ccagaattga cctccccaaa aattcccgtg
     5581 accgtgctga cgggctatct cggcgccggc aagaccacgc tgttgaaccg catcctgtcg
     5641 gaaaaccacg gcaagaaata cgccgtcatc gtcaatgaat tcggcgagat cggcatcgac
     5701 aacgatttga tcatcggcgc cgacgaagaa gtctttgaga tgaataatgg ctgcgtgtgc
     5761 tgcacggtgc gcggcgatct cgtgcgcatc ctggacggcc tgatgaaacg caaaggcaaa
     5821 ttcgacgcca tcatcgtcga gaccacgggt ctcgccgatc cggcgccggt ggcgcagacc
     5881 ttcttcgttg acgaggatgt gcagaagaat gcgcggctcg acgcggtggt gacggtggcc
     5941 gatgcgaaat ggctgaacgc tcggctcaag gacgcgccgg aagccaaaaa ccagatcgcg
     6001 ttcgccgacg tcatcgtgct gaacaagacc gatctcgtct ccaaggcgga actggccgaa
     6061 gtcgaggcgc ggatcagagc catcaatcct tatgccaaat tgcatcgcac cgagcgctgc
     6121 caggtcgcat tgtcggatgt gctggagcgc ggcgcgttcg atctcgaccg catcctcgaa
     6181 ctcgagccgg aatttttgga cgccggcgac gaccatgatc atcatgacca tgatcaccac
     6241 ggccacggac atgatcacgg ccatagccat ggcctgaaac attaccatga cgaggatatg
     6301 caatcgctgt cgctgcgcag cgacaagccg ctcgatgcaa caaaattcat gccctggctg
     6361 cagaacctgg tcgccagcga gggccagaag atcctgcgct caaaaggcat tctcgccttc
     6421 agcgacgatg acgaccgcta tgtcttccag ggcgtgcaca tgatgctgga gggcgatcac
     6481 cagcgggcct ggaaagacgg cgaagcgcgc gagagccgcc tcgtcttcat cggccgcgaa
     6541 ttgcccgagc agaccatccg tgacggtttt gagagctgta tcaccacgtg atgaaagagt
     6601 tcgatcccgg cgataatgcc gcctccatcg tttcggtcac cgaccgggtg cgcccggtcg
     6661 cgatcggcat gccggtcacc tcggtgcatt tcctcggcga gcgcgcggcc ttcgtcggcg
     6721 ccgaggaaag cgttgcgatg gtgagcgcgg acggtgaaat tttgccggtc gccgtccacg
     6781 gtggcggaat tctgagcgcg gcatccgacg gcgagcgcat cgtcatgggt ggcgatgacg
     6841 gcaagctggt tgcgctggac gctaaaggcg aggtggcgtt gatcgccacc gacccgaaac
     6901 ggcgctggat cgacaatgtc gcgctgcatc ccgatggcgc ggtggcgtgg tcggccggca
     6961 agacggcgtt tgtccgcggt ggcaagggcg aagaaaaatc gttcgatgtg ccgtcgaccg
     7021 tcggcgggct ggcgtttgca ccaaaaggcc tgcggctcgc cgtggcgcat tacaacggcg
     7081 tgaccctgtg gtttcccaac atggtggcga cggccgaagt gctggaatgg gcgggctcgc
     7141 atctcggcgt cgtgttcagc ccggacaaca agtttctggt gactgcgatg cacgagccgg
     7201 cgctgcatgg ctggcggctg gccgatcacc ggcacatgcg catgaccggt tatcccggcc
     7261 gcgtgcggtc gatgtcgtgg agtgccggcg gcaaagcgct ggcgacgtca ggcgccgata
     7321 ccgtgatcct atggccgttc gccagcaagg acggcccgat gggcaaggag cccgcgatgc
     7381 tggcgccgct gcaggcccgc gtctccatgg tcgcctgcca cccaaaacag gatattttcg
     7441 cggcgggcta cagcgacggc accattttga tggtgcggct gaccgacggc gcggaaatcc
     7501 tggtgcgcaa acccggtgcc gtgccggtcg cggcgctggc ctggaacgcc aagggcacgc
     7561 tgttggcatt cgccgcggag gatggcgacg cgggtttgct cgagctttag cgtctgaccg
     7621 ccttgctgcc cgcctagcct aataggtgtg tcatcagtca cttgatcgta cccatattgg
     7681 ggtgtatatt ccccaatatg ggtacgatga gcacggacgc gataagtcta tcaaacgcgt
     7741 tgttttccaa ggtgcagcaa cgtgtgctgg ccctcatctt tggtcagcct gagcgcagct
     7801 tctatacgtc cgagatcaag aggaacgtta actcgggaac gggcgccgtt gaacgagagt
     7861 tgtcgcggct gcagcgcagt ggactcgtct ccatagagcg catcggtaat cagaaacatt
     7921 accgggctaa tcgtcagtct ccgattttcg cggaactaca tagtcttgtt atcaaaaccg
     7981 tggccttgac cgagcctctc aggaagtccc ttgagcccta ttctgataag atcaaggctg
     8041 cattcgtgta cggttcggtg gcaaagggca cggatacggc acgcagcgac atcgatctct
     8101 tggtgatcgg ggacgagttg agctactccg aactctatgc tgcgttacag aatgtcgaga
     8161 acgcattggg tcgcaaggta agcccaacgt ttctttctcc aaaggactgg cggcgcaagg
     8221 cttctgacag aggttcattc atcagcaaag taaatgcatt gccaaagatt tttgttttcg
     8281 gttcagagaa agacctccag ctatgagcaa ggaggaactc gacaacctcg taaggatcaa
     8341 ccggctcaaa gccgagccag gttcacgcgg tgagtttgct ggcatgctaa attccgcccg
     8401 cacacgtctg accgatgccc agaacgagag tcttgatccc gacagccaat ttgacctggc
     8461 gtacggcgcg gcgcatcggc tggcgctcgc tgcactacgc caccaaggat accggtcgga
     8521 aaatcgcatt actgttttcc agaccctcgt tcatacgttg ggttcggata gtgctgacgt
     8581 gcaaattttt cttaaggccc acaacgaacg aaaccttgca gaatacgaag gccgtatgga
     8641 gattgacgaa ggtcttttgg ccgatctaat ccgctgcaca aaaaaaatag agacagcggt
     8701 aggaaaactc gatccgccca ccggcgaatg aagcccaggc agctacgccg ggagaaggtc
     8761 taccgccggg gtcaccggta aaatgccgcc cggaaggccg ggccgggttc ccaagggaac
     8821 aatgcgttcc gccgtccgtt gacctcacca acgggatgcg gtgaaccata tgtcttccga
     8881 cgacaggaaa cgcgcttaca ggatggattt gctcgtcgcc tctgcgatgg tcgcggcggg
     8941 gcttgctgta tccggcgctt cgctggtcct gctcaacgcc gaaaccacgc agatggcgca
     9001 ggccacgcca ccgttgcagt cgacaccggg cgccgaaacc aaaccgtcgg cgcctgccga
     9061 gcccgtgacg accggcgcgc ggccaagcca gattccaccc gaacctgccc ggccggatgc
     9121 cgacgcggag aaggccggcg ccacgccggc gctgccgccg gctccagccg aaaagaccgc
     9181 ggcaccggtc aaggacagat agcctcgtca ttccggggct cgcgctagcg agagcccgga
     9241 atcccgagat gattggcgcg agattccggg ttcgcgcttt cagcgcgccc cggaatgacc
     9301 gccttctatc acttgggtcg tcgctgccga atttacagtc tcgcctgtgt caccttgctg
     9361 ccgcataatg tcgtcaagcg cggcagcgtg gatgatacat gaggcaggcg atgaagatcg
     9421 acgacgtcag gcgaaccgct tattcgatgc cgctcaccaa cccgtcgttc ccgccggggc
     9481 cttatcgctt tttcaaccgc gaatatttcg tcatcaccta ccgcaccgat ccggaggcgc
     9541 tcgccgccgt ggtgccggag ccgctcgaag tcactgaacc catcgtcaaa tacgaattca
     9601 tccggatgcc ggattcaacc ggttttggcg actacaccga aaccgggcag gtgattccgg
     9661 ttcgactgaa gggtgaggag ggcgtctata cccacgcgat gtatctcgac gacgatgcgc
     9721 cgatcgccgg cggccgcgag ctgtggggat ttcccaagaa gcgggctacg ccgaagatcg
     9781 aggtcgagag cgacgtgctg gtgggatcgc tgcattacgg ctcggtgctc tgcgcctccg
     9841 ccaccatggg ctacaagcac cgcccgatcg atcatgacac cgtgctcaag agcatgaagg
     9901 cgccgaattt catcctcaag atcattccgc atgtcgacgg cagcccccgg atctgcgagc
     9961 tggtgcgctt ctctctcgag gacatcacgt tgaaggaggc ctggaccggc ccggcggcgc
    10021 tcggcttgtt cccgcacgcg ctcgccgacg tcgcgcggct gccggtgcgc gaggtggttt
    10081 ccgcgctgca tttcaaggcg gatctcacgc tgggcctcgg caaggtggcg ttcgattatc
    10141 tggcgaagta accacttgcc gtcattccgg ggcgatgcgc agcatcgaac ccggaatctc
    10201 gagattccgg gttcgcgcta acgcgcgccc cggaatgacg gtagtacggc gtcaccgctt
    10261 caccgtacca gcacattccg aaactgccag ggatcgcttt cgtcgatatc ttccggaaac
    10321 agcccgggcc ggtcgctgag cggggtccag tcggtgtaaa agcctttgac cgggccgaga
    10381 tacgaccgct ggatttcaag gcagcggctg aaatccatct cgtcggcctc gacaatcccg
    10441 gcggtcggat tttccagcgc ccacaccatg ccggcgagca ccgccga
//
LOCUS       DASWGM010000664         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1169202,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000664 DASWGM010000000
VERSION     DASWGM010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169202"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VGA15_18705"
     CDS             <1..1168
                     /locus_tag="VGA15_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9589761.1"
                     /translation="RQIETVVKAVPGTSSAFAERVIGGYYLDVVPDREALARYGLMVS
                     DVQDTVSSALGGETITTTVEGRERYGVNLRYPRDLRSDPQSIARDVLVALPGGGTVPL
                     GEVAAIKLTRGPTSIRTENGQLAVYIFVDIRDRDLGGYVADAKAAVAASVQFPPGNYV
                     VWSGQFEYLERATARLKTLVPVTLLIIFLLLYLNFRRLTETLIVMLSLPFALVGGLWL
                     MWWLGFNFSVAVAVGFIALSGVAAEIGVIMLIYLDHAMKELHAERLAEGRPFTRTDLN
                     AAIMLGTVERVRPIAMTVVAIMAGLIPIMWSTGTGSEVMQRIAVPMIGGMISATILTL
                     VVIPAVYAFVKGFGLPREPGDAVPVELGMPDRTPKALSIVRKPRNDWKRDEKRI"
     gene            1174..>1610
                     /locus_tag="VGA15_18710"
     CDS             1174..>1610
                     /locus_tag="VGA15_18710"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX9589762.1"
                     /translation="MREREIEWTELMQSANAGDGAAYHRLLMGIAPVLRITVEGGLER
                     AGRPTDQCEMIVQEILLAVHLKRHTWDVSTPFCSWLFAITRNKLLDVMRRSDKPGRID
                     IDCFRDPSPEEPPSESRKTTEVDEHIQSLSHRQRIVLQAIVSG"
BASE COUNT          293 a          462 c          515 g          340 t
ORIGIN      
        1 acggcagatc gaaacggttg tgaaggcagt tccgggcacc tcgagcgcct ttgctgaacg
       61 tgtcatcggc ggctactatc tggatgtcgt acccgaccgg gaagcgctcg cgcgctacgg
      121 tctcatggtc agcgatgtgc aggacacggt ctcgtccgcc ctcggcggcg agaccatcac
      181 cacgaccgta gagggtcgtg agcgctatgg cgtcaatctg cgctatccgc gcgatctgcg
      241 cagcgatccc caatcgatcg cgcgagatgt gctggtggcc ctgccaggcg gcgggactgt
      301 cccgcttggt gaggtcgcgg cgatcaagct gacgcgcggc ccgacctcga tccggaccga
      361 gaacggccaa ctcgccgtct acattttcgt ggatatccgc gaccgcgatc tcggcggcta
      421 cgtggccgac gccaaggcgg ccgtggccgc cagcgtgcag tttccgccag gaaattatgt
      481 cgtctggagc ggtcagttcg aatatctgga gcgcgccacc gccaggctga agactttggt
      541 gcctgtcacg ctgctgatca tcttcctgct gctctatctg aatttccgaa ggttgaccga
      601 gacactgatc gtcatgctgt cgctgccgtt cgcgctcgtc ggcggcctat ggctgatgtg
      661 gtggctcggc ttcaattttt cggttgcggt tgccgtgggc ttcatcgctt tgtccggtgt
      721 cgcggcggag atcggcgtca tcatgttgat ctatctcgat cacgcgatga aggaactcca
      781 cgcggagcgg ctcgcggaag gcaggccgtt tacgcgaacg gacctgaatg cggccatcat
      841 gctcggcacg gtcgagcggg tgcgacctat agcgatgacc gtcgtcgcca tcatggccgg
      901 cctcattccg atcatgtgga gcacgggcac cggctccgag gtcatgcaac ggattgccgt
      961 gccgatgatc ggcggcatga tctcggcgac catcctgacg ctcgtcgtga tcccggctgt
     1021 ctatgcgttc gtcaagggat ttgggttgcc gcgcgaaccc ggtgatgccg ttccagtgga
     1081 attgggtatg ccggatcgga ccccgaaagc tctctcgatc gttcgcaagc cgcggaatga
     1141 ttggaagcgc gatgaaaaga ggatctgaag cgcgtgcgcg aacgtgaaat cgaatggacc
     1201 gagctaatgc agtcggccaa tgccggcgac ggtgccgcat atcaccgtct tctaatgggg
     1261 attgcgccgg ttttgcggat aactgtggaa ggtgggttgg agcgggcggg acggccgaca
     1321 gatcagtgcg aaatgatcgt gcaggaaatc ttgctcgctg tgcacctgaa gcgtcacact
     1381 tgggatgtga gtacgccctt ttgttcctgg ctattcgcga ttacccgcaa caaactgttg
     1441 gacgtgatgc gccgaagcga caagccgggc cgcatcgata tcgattgttt cagggatccc
     1501 tcacctgagg agccgccctc ggagtcgcgt aagactactg aagttgacga gcatatccaa
     1561 agtctgtcgc acaggcagcg cattgtcttg caagcgatcg tctccggaca
//
LOCUS       DASWGM010000665         8237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2442042,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000665 DASWGM010000000
VERSION     DASWGM010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442042"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VGA15_18715"
     CDS             complement(<1..543)
                     /locus_tag="VGA15_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9589763.1"
                     /translation="MSPLRRLLGNIGLAFALIVIVSPAVLFFVWMLSLSVKFEIDNAS
                     YPPVFIPERFNWGNYTAVLDSKRFSTYFMNSLIVTGTATAFALLVGVPAGYGLARMRA
                     HKAAVVILIARITPGLSYLIPLFLLFQWLGLLGTLWPQIIIHLVVTVPIVIWIMIGYF
                     ETTPMELEESALIDGATSWQV"
     gene            complement(540..1478)
                     /locus_tag="VGA15_18720"
     CDS             complement(540..1478)
                     /locus_tag="VGA15_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004680330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9589764.1"
                     /translation="MSTIAEPAEVTAGAKGEPQREWRPPSYWPFVAPALIVVLAVIIF
                     PWAYTIWMSLHEWKVGSPTTFVGLANYIRLPSDARFVESVWHTLVYTALSVALPLLFG
                     TLAAVVFHAKFPMRGFLRGLFILPMMATPVAIALVWTMMFHPQLGILNYLLSLIGLPP
                     QLWVFHPATVIPSLVMVETWQWTPLVMLIVLGGLAAIPSEPYESALIDGATRWQIFRY
                     ISLPLITPFLFIASMIRMIDAVKSFDIIFAITQGGPGSASETINLYLYSVAFVYYDVG
                     YASAIVVVFFALIVALAGALLHLRQRTHWNDAGGVA"
     gene            complement(1475..2800)
                     /locus_tag="VGA15_18725"
     CDS             complement(1475..2800)
                     /locus_tag="VGA15_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9589765.1"
                     /translation="MKDTLVSRRAVLAGTAAAGALGLSGFPARAEPQWKKYAGTTIEA
                     NLIKGPRGELLQKYASEFTDLTGIKVESEVIPEQQQRQKAVIELTSGKPSFDVIHVSY
                     HVQKRQFEKAGWLADLTAFMKDPNLTAPDLTVDDFSAGGLQFAQNDKGEMHSLPWSVD
                     YFILYWNKELFAKKGVALPKTFDEMAVAAEKLNDPANGIYGFTGRGLRNANMTLWTNF
                     FLNYGGEFLDDKGNILTDGPEAIEATKMYQRLLTKTAPPGVAGFNWMESMASLTQGRA
                     AMWIDGVGWAPPIEDPNASRVVGKIGYALVPAGPKGQYSAAYGDGVGIAQASTKKEAA
                     YLYCQWAVSKTMGARLLQSGGGVPFRNSVLNDDAVRKGVKMPPEWLDSVIGSAKISKL
                     GLPVIVPVAEFRDIVGAAVTATLSGADPATELKKAHEQFRPILERSEKS"
     gene            3233..4156
                     /locus_tag="VGA15_18730"
     CDS             3233..4156
                     /locus_tag="VGA15_18730"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9589766.1"
                     /translation="MKASPFGPDDQTGATNRITPAVTKAAAAEIQTGAVIPMAYNLVD
                     GVPLFGTRFTKTILTSVALVPGAEFGKNKLTYMEDTYLSQSHVGTHIDGMGHIGIMDC
                     YYNQTPMGKFVTQNYLKKLGLENLKTFATRGVVIDMVKVFQDAGKLKTNPACRTPCLD
                     GGTVITEADIQAGLKMYNVSLREGDAVFLHTGWGDLFTQFPAQNATYNKAEPGIGKSA
                     AQWLADQKVVLVGTDTWAVEVIPGEDANEAFPVHALLLTDNGIHIIENVRTDLIAADA
                     ASSKRATFFLSMTVPKAVGTTGTFVNIEAIR"
     gene            4206..5204
                     /locus_tag="VGA15_18735"
     CDS             4206..5204
                     /locus_tag="VGA15_18735"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9589767.1"
                     /translation="MKRRHFLLAILAVASSARAANGEAQRFRVAFANLNEEPGTSIEG
                     LGFSGAEIRRSFELASRGLPLDMIYYDNGGDAGKALANAADAVSRKVDLLIEYNSDAE
                     ANAEIGRQLKTAGIPVLALNHPIPGAPLYTADHLAAGRIAGRTLGQFAKENWSDQMPV
                     AAILGDVADPSPSVAARIQGVTEGLRQELPNVPPILLDSAGHPVRAEGVLAKFLGTQA
                     RRKVLVATLDDASALAAKSAIETAGRTSDCAIVSQGASRIVHGGASEKKEIDPANRGS
                     IVLGSVAYYLDRYGYEVLPIAVRMLRGEQVPARTLTSHVLISAKNVFREYPPYDMN"
     gene            complement(5223..6566)
                     /locus_tag="VGA15_18740"
     CDS             complement(5223..6566)
                     /locus_tag="VGA15_18740"
                     /inference="COORDINATES: protein motif:HMM:TIGR05002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat
                     NxxGxxAF-containing protein"
                     /protein_id="HEX9589768.1"
                     /translation="MNTPGMSPLGGPLLASASSRLTIAGAAILLLWIAVLWASLSEPT
                     RKAALQNAAPEVPALRLVVASGQAAPSGGRFDRFDVSSQPIVAPVNGRGQVAFYASVA
                     RAKATEGIFLATASRVTKAAAVGDAVPGGGVLSEFARHPVPALNDAGKIAFGANVAGA
                     RATEGIFLAADDRLKAIALSGTDAPGVQNGTFVEFDAPVLNNRDEIAFVGTVRRGRET
                     LQVLYLYSGGVLRKLVAGGDPAPRGGSFDRFGVPAINNKGVVVFPAVIEQGAVLGGIF
                     VAGARELRLLVGAGEIGPGGAMLVRFSERAAIDDEDNVAFVAHLGSGTSAEAVLVANA
                     SGLTQLAIVGENAPGGGRFAAFGPWPNFGPNATGAFVAAIDGGPGTLGIYLGTPGAIH
                     RVAMVGDRMANGGVIPALALNSVTSVGSRGGLTFATMANPESGQNGIYYYGPPGS"
     gene            complement(6571..7506)
                     /locus_tag="VGA15_18745"
     CDS             complement(6571..7506)
                     /locus_tag="VGA15_18745"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX9589769.1"
                     /translation="MKRDASISTPFRFVLVCVGILLGIGPAAAADKIKVVATFSVIGD
                     MVANVAGDRVDLVTLVGPAGDTELYKPSLADGKSVAEAKIVFMNDLNDEFEPWLEPLL
                     KGVKFGGTKIVVSRGAKTYSSSEERAPRSKAAEEIDQHAWLDPKNGIIYVRNIAAALA
                     RSDPANAPDYRARAAAYIKELQDLDAWAKAEMAAVPPAKRRILSSHDSLQYLARAYGI
                     TMLAINGWTNNSEASAAELASLTQKIRQEGIHALFLDSITDPRAMQQVAQETGAAIGG
                     TLYGDALSKPGEEADTYLKMLRHDVTTLIAGMMKN"
     gene            complement(7503..>8237)
                     /locus_tag="VGA15_18750"
     CDS             complement(7503..>8237)
                     /locus_tag="VGA15_18750"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX9589770.1"
                     /translation="SLSGNVLSHAVMPGAALGYLYAGYSLAALSFGGLLTGLVVAGLA
                     ALKTRSKPQRQDASLIAFYLVSLSFGILIVAARGSQADLMHVLFGTVLAVDVAALLLI
                     ASITSLTLLVIAALYRPLAVESFDPAFLRAAGGGGGVFRAIFITLVVLNLVASFQAFG
                     TLLAIGPMLLPAAAARCWTQKVWPMIALATVIGMAAALAGLLISYYANLPSGPAIVMG
                     AGLIYGISLLAAGRTRIKPRVSEEQR"
BASE COUNT         1665 a         2565 c         2536 g         1471 t
ORIGIN      
        1 gacctgccag ctggtggcgc cgtcgatcag cgccgactct tccagctcca tcggggtcgt
       61 ctcgaaatag ccgatcatga tccagatcac gatcggcacc gtcaccacga ggtgaatgat
      121 gatctgcggc cagagcgtgc ccagcaagcc aagccactgg aacagcagga acagcggaat
      181 gagataggac aggccgggcg tgatgcgcgc gatcaggatc accaccgcgg ccttgtgcgc
      241 ccgcatgcgg gcgaggccat agcccgcggg cacgccgacc aatagtgcga acgccgtcgc
      301 agtgcctgtc acgatcaggc tgttcatgaa gtaggtcgag aacctcttgg agtcgagaac
      361 cgcagtatag ttgccccagt tgaaacgctc gggaatgaaa accggtggat acgacgcgtt
      421 gtcgatctcg aatttgacgg ataacgacag catccagacg aagaacagca ccgccggcga
      481 cacgatgacg atgagtgcga aggcgaggcc gatgttgccg agcaagcgtc gcagcgggct
      541 catgccacgc ctccggcgtc gttccagtgc gtcctttgac gcaggtgaag cagcgcaccg
      601 gcgagcgcga cgatcagcgc gaagaacacg accacaatcg cggaggcata tccgacgtcg
      661 taatagacga aggcgacgct gtagagataa aggttgatgg tttccgaggc cgagccggga
      721 ccgccctggg taatcgcgaa aatgatgtca aaactcttca ccgcgtcgat catgcggatc
      781 atcgacgcga tgaacaggaa tggcgtgatc agcggcagcg agatgtagcg gaagatttgc
      841 cagcgggtgg cgccgtcgat cagcgcgctc tcgtaaggct cgctcgggat cgccgcgagg
      901 ccgccgagca cgatcagcat caccagcggt gtccattgcc aggtttcaac catcaccagc
      961 gacgggatca cggtcgcggg atgaaacacc caaagctgcg gcggcaggcc gatgagcgac
     1021 aagaggtagt tgaggatgcc gagttggggg tggaacatca tggtccacac cagcgcgatg
     1081 gcgaccgggg tcgccatcat cgggagaata aagaggccgc gcagaaaacc gcgcatcgga
     1141 aatttggcgt gaaacaccac cgccgccagc gtgccgaaca gcagcgggag cgcgaccgac
     1201 agcgcggtat agaccagcgt gtgccacacc gattcgacga agcgcgcatc gcttggcagg
     1261 cggatgtaat tcgccagccc gacaaaggtg gtgggcgagc cgaccttcca ttcgtgcagg
     1321 ctcatccaga tcgtgtaggc ccacgggaag atgatgaccg cgagaaccac gatcagcgcc
     1381 ggcgccacga agggccagta ggacggcggc cgccactccc gctgcggctc gcccttggcg
     1441 ccggccgtga cctcagccgg ctcggcaatc gtgctcacga cttttcgctg cgctccagaa
     1501 tcggacggaa ttgctcgtgc gcctttttca gttccgtcgc cgggtccgcc cccgacagcg
     1561 tcgccgtgac ggcggcaccg acgatgtcgc ggaactccgc gaccggaacg atcacgggca
     1621 ggccgagttt cgaaattttg gccgagccga tcaccgaatc cagccactcc ggcggcattt
     1681 ttacgccctt gcgcacggca tcgtcgttga gcacggagtt acggaacggg acgccgccgc
     1741 cgctctgcaa gagccgcgcg cccatggtct tcgacaccgc ccattggcaa tagagatagg
     1801 cggcttcctt cttcgtgctg gcctgggcaa tgccgacgcc gtcgccatag gcggcggaat
     1861 actggccttt gggtccggcc ggcaccagcg cgtagccgat cttgccgacg acacgcgagg
     1921 catttggatc ctcgatcggc ggcgcccagc cgacgccgtc gatccacatc gccgcgcggc
     1981 cttgcgtcag cgaggccatc gactccatcc agttgaaacc ggcgacgccc ggaggcgccg
     2041 tcttggtcag caggcgctga tacatcttgg tggcttcgat cgcttcgggc ccgtcagtca
     2101 gaatgttgcc cttgtcgtcg agaaactcgc caccgtagtt gaggaagaaa ttggtccaca
     2161 gcgtcatgtt ggcgttgcgc aagccgcggc cggtaaagcc atagatgcca ttcgcgggat
     2221 cgttgagctt ctcggcggcg acagccatct cgtcgaaggt tttgggcaat gcgacgccct
     2281 tcttcgcgaa cagctccttg ttccagtaaa ggatgaaata gtcgaccgac cagggcagcg
     2341 aatgcatttc gcccttgtcg ttctgggcga attgcagacc gccggcggaa aagtcgtcga
     2401 ccgtgaggtc gggcgcggtc aggttcgggt ctttcatgaa cgcggtcagg tccgcgagcc
     2461 agccggcctt ttcgaattgc cgcttctgca cgtgatagct gacgtgaatg acatcaaaac
     2521 tcggcttgcc ggaagtcagt tcgatgacgg ccttctgccg ctgctgctgc tccgggatga
     2581 cttcggattc gaccttgata ccggtaaggt cggtgaactc ggacgcgtat ttctgcagca
     2641 attcgcctct tggccccttg atcaggttgg cctcgatggt ggtgccggca tacttcttcc
     2701 attgcggctc ggcgcgcgcg ggaaatccgg aaaggccgag cgctcctgcc gccgcggtgc
     2761 cggccagaac ggcacgacga gaaacaaggg tatccttcat tggtcttctc cctggaggtc
     2821 ggcttatttt gacgggatgt tagcgcctgc gcggtttcgg caacagcccg atcatgatgt
     2881 atgcggttgc tttgtttggt tttctccaag gcgccttgca tccgcagcag cacctcaaac
     2941 cgcattgcac aaagtgccac tgaatcggcg aaatgacccg cgcgattgca gcctccgaaa
     3001 tggttccgcc gctcaaatac gaccaaaatc agagaagact caagacgccg attgaaacgg
     3061 cttgcgaggc ctgccatgat tgctgaccgt aatgtgcaga ttcaactgca ccgacagatc
     3121 cagggaggcg atcatgaaat acacttcgat cgttgcaagt gtcatcttat cggcgttagc
     3181 gctcttgggt agtttgaacg tcactctcgc gcagagtgat tgcaagactt tggtgaaagc
     3241 atctcctttc ggtccggacg atcaaactgg cgcgaccaac cggatcaccc ccgccgtgac
     3301 caaggccgcc gccgcggaaa tccagaccgg cgccgtcatt cccatggcct acaatctggt
     3361 cgatggcgtg cctctgttcg gcacccggtt caccaagacg atcttgacga gcgtcgcgct
     3421 cgttcccggc gccgaattcg gcaagaacaa attgacctac atggaagata cctatctcag
     3481 ccaaagccat gtcggtacgc atatcgacgg catgggtcac atcggcatca tggattgcta
     3541 ctacaatcag acgccgatgg gaaagttcgt cactcagaac tacctgaaaa agctggggct
     3601 tgaaaacctc aagactttcg ccacccgcgg cgtagtcatt gacatggtca aggtgttcca
     3661 ggatgccggc aagctgaaga ccaatccggc ctgccggacg ccgtgtctcg acggtggcac
     3721 ggtgatcacg gaagccgaca tccaggccgg tctgaaaatg tacaatgtct ccctgcgcga
     3781 aggcgatgcg gtgttcctgc ataccggatg gggtgacctg ttcacccagt tcccggcgca
     3841 gaacgccacc tataacaagg ccgagcccgg catcggcaaa agcgcagcgc aatggctcgc
     3901 cgatcaaaag gtggtgctgg tcgggaccga cacctgggcg gttgaggtga ttcccggcga
     3961 ggatgccaac gaagccttcc ccgtccacgc gctgttgctg acggataacg gcatccacat
     4021 catcgagaac gttcgtaccg atttgatcgc cgccgacgca gcgagcagca aacgcgcgac
     4081 cttcttcctc tcgatgacgg tcccgaaggc ggtgggcacc accggcacct tcgtcaatat
     4141 cgaagcaatc aggtagtgtg tctgcagcgg atcttgacga acgcaacgtt gcgctggcgc
     4201 ttcacatgaa gcgccggcat ttcctgctgg cgatactcgc cgtcgcctcg tccgcgcgcg
     4261 cggcaaacgg ggaggcccag cgctttcggg tggcgttcgc caatctgaac gaggagccgg
     4321 gcacgtcgat cgagggcctg ggctttagcg gggcggaaat ccgccgcagc tttgagctgg
     4381 cgtcgcgcgg cctgccgctg gacatgatct attacgacaa tggcggcgac gccggcaaag
     4441 cccttgccaa tgcggcggat gccgtttccc gcaaggtcga cttgttgatc gaatataatt
     4501 ccgacgcgga agccaacgcc gaaatcggcc gccaactgaa aacagcgggc attccggtac
     4561 tcgcgttgaa ccatcccatt ccgggcgcgc cgctttacac ggccgatcat ctcgcggcag
     4621 gcaggatcgc cggccgaacc ctcggacaat tcgccaagga gaactggtcc gatcagatgc
     4681 cggtcgccgc gatcctcgga gatgtcgccg accccagccc gtccgtcgcc gcgcgcatcc
     4741 agggagtgac ggaggggttg cggcaggaac tgcccaatgt tccgccgata ttgttggaca
     4801 gcgccggcca tccagttcgt gccgagggcg tcctcgccaa gtttctcggc actcaggcca
     4861 ggcgcaaagt gcttgtcgcg acgctcgacg acgcctccgc cctcgctgcc aaatcggcga
     4921 ttgaaaccgc cgggcgcacc agtgactgcg ccattgtgag tcaaggcgct agccgcattg
     4981 tccatggcgg cgcgagtgag aagaaggaga tcgatccggc caatcgcggc agcatcgttc
     5041 tgggttcggt ggcctattac cttgaccgct atggctatga ggtcctgccg atcgcagtgc
     5101 ggatgctgcg gggtgagcag gtgccggcgc gaactctgac cagccacgtg ctgatttccg
     5161 caaagaacgt gtttcgcgaa tatccgccgt atgacatgaa ctaagctcag ctctaaccaa
     5221 gctcagcttc ccggtggccc gtagtaatag atgccgttct gaccgctttc cggattggcc
     5281 atggtggcga atgtcaggcc accgcgcgaa ccgaccgagg tgacggagtt gagtgcgaga
     5341 gcggggatga cgccgccatt ggccatccgg tcgccgacca tggcgacgcg atgaatggct
     5401 ccgggggttc cgagataaat cccgagggtc cccggcccac catcgattgc cgcaacgaag
     5461 gcgcctgtgg cgttggggcc gaaattaggc catggcccga acgcggcgaa gcggccgccg
     5521 cccggcgcgt tctcccccac gatcgccaac tgcgtcaacc cgctggcatt cgccaccagc
     5581 acggcttcgg cagaggtgcc cgagccgaga tgcgcgacga aggcgacatt atcctcatca
     5641 tcgatcgcgg cccgctccga aaagcgcacc agcatcgcgc ctcccggtcc gatctcgcca
     5701 gctccgacca gaaggcgcaa ttcgcgggcg ccggcgacga agattccgcc cagtaccgca
     5761 ccttgctcga tgaccgcggg gaagacgacc acgcccttgt tgttgattgc gggaacaccg
     5821 aagcggtcga agctgccgcc gcgtggggct ggatcaccgc ccgccaccag cttgcgcaac
     5881 acgccgccac tgtaaagata aagcacctgc agcgtttccc ggcctcgccg gactgttccg
     5941 acaaaagcga tttcatcccg attgttaagc accggcgcat cgaattcgac gaaggtgccg
     6001 ttttgcacgc cgggtgcgtc ggtcccgctc agcgcgatag ctttgaggcg gtcgtcggcg
     6061 gcgagaaaaa tcccctcggt ggcgcgcgct ccggcaacgt tcgcgccaaa tgcgatcttg
     6121 cccgcatcgt tcagcgccgg aacgggatgc cgcgcaaatt cggacagcac gccgccccca
     6181 ggtaccgcat cgccgactgc cgccgctttc gtaacccgtg acgcggtggc aagaaaaata
     6241 ccttcggtcg ccttggcccg cgccacgctg gcgtaaaagg caacctggcc tcgaccgttg
     6301 accggtgcca cgatcggctg cgagctgacg tcgaaacggt cgaaacgacc gccacttggt
     6361 gcggcctgtc cggaggcgac gactaggcgc agggcaggca cctccggagc cgcattctgc
     6421 aaagccgcct tgcgggttgg ttccgacaga ctcgcccata atacggcgat ccacagcagc
     6481 aatatcgccg cacctgcgat cgtcaggcgg gaactcgcgc tcgccaggag tggtccgcca
     6541 agcggcgaca tgccgggggt attcatcttg tcaatttttc atcatgccgg cgataagcgt
     6601 cgtcacgtcg tgacgaagca ttttgagata agtgtcggcc tcttcgcctg gcttcgacag
     6661 cgcgtcgcca tagagcgtac cgccaatggc cgcgccggtc tcctgggcga cctgctgcat
     6721 ggcacgcgga tcggtgatgc tgtcgaggaa cagcgcgtgg atgccctcct gtcggatctt
     6781 ttgagtaaga ctagccagct cagccgcgga cgcctcgctg ttgttggtcc agccattgat
     6841 cgctagcatc gtaataccgt aggccctggc aagatattgc aatgaatcgt gcgaagagag
     6901 gatgcgccgc ttggccggcg gtaccgccgc catctcggct ttcgcccagg catcgagatc
     6961 ctgcaattcc ttgatgtagg cggcggcccg cgcgcgatag tccggggcgt tggccggatc
     7021 gcttctggcc aacgccgcgg cgatgttcct gacgtagatg atcccgttct tcgggtcgag
     7081 ccacgcatgc tggtcaatct cttcggccgc tttgctccgc ggggcgcgct cctcgctgga
     7141 gctgtaggtt ttcgcgcctc tgctgacgac gattttggtg ccgccgaact tgacgccttt
     7201 gagtagaggt tcgagccagg gttcgaactc gtcgttgaga tcgttcatga agacgatttt
     7261 ggcttcggcg acgctcttgc cgtcggccaa ggatggcttg tagagctcgg tgtcgccggc
     7321 gggtccgacc aaggtaacaa ggtcgacccg atcgccggcg acattcgcga ccatgtcgcc
     7381 gatcacgctg aacgtcgcca cgaccttgat cttgtcagcg gccgcggccg ggccaatgcc
     7441 gagcaggatc ccgacacaaa caaggacaaa ccgaaacggt gtgctgattg aagcgtcgcg
     7501 tttcatcgct gttcctccga aaccctgggt ttgatccgcg tccggccggc cgccagcagc
     7561 gaaatgccgt agatcagccc cgcgcccatc acaatagccg gccccgaggg caggttggcg
     7621 taataagaga tgagaagccc cgcaagcgcg gcggccattc cgatcaccgt cgcaagggcg
     7681 atcatcggcc atactttctg ggtccagcac cgcgccgccg ctgcaggcag cagcatcggc
     7741 cctatcgcaa gcaaggtgcc aaaagcctgg aaacttgcca ccagattgag aaccaccagc
     7801 gtgatgaaaa tggcgcggaa tacgcctccg ccgcccccgg ccgcgcgcag gaacgccggg
     7861 tcgaaggatt cgacggccaa aggacgataa agcgctgcga tgaccagcag agttaggctg
     7921 gtgatggagg caatcagcag cagcgcagcg acgtcgacag ccaacaccgt gccgaaaaga
     7981 acatgcatca gatccgcctg gcttccccgc gcggcgacga tcagaatccc gaacgacagc
     8041 gacacgagat agaaggcaat caaactggcg tcctggcgct ggggctttga ccgtgtcttg
     8101 agcgccgcca ggccggcgac gacaaggcca gtcagcagac caccgaaact cagggcggcc
     8161 agggaatagc cggcgtagag atagccgagt gctgcgcccg gcatcaccgc atgcgacagt
     8221 acattcccgc tcaggct
//
LOCUS       DASWGM010000666         3410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_753924,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000666 DASWGM010000000
VERSION     DASWGM010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753924"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..333)
                     /locus_tag="VGA15_18755"
     CDS             complement(40..333)
                     /locus_tag="VGA15_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX9589771.1"
                     /translation="MTTYIVLVNWTERGIQQVKDSPRRFDAAKKMLKEMGGEIKSVHL
                     TMGEYDLVLVCEASDDAVAARFALQLGIMGNVRTRTLKAFPEAAYREIIASLG"
     gene            complement(444..1700)
                     /locus_tag="VGA15_18760"
     CDS             complement(444..1700)
                     /locus_tag="VGA15_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589772.1"
                     /translation="MNHRGHRLAAALLALAIALPAGAAETVKIGVIYPLSGNAASAGN
                     SAKDAIELGAEIVNGVYPELKGSPLAGAAGLANLGGAKVELLNADHQGNPSVGQSQTL
                     RLVTQEKVAAMLGAYHSSVALTATAVAERYGVPFLVADSVALNITQRGFKWIFRSGPI
                     APDFAKAYTSFLGELKQAGRKVDAIAIVNENTDYGTSVAASISDAANKSGIKVAAQIP
                     YNANGSDVSGQVLQLKQLAPDAVIFISYTADTILYFKTLKNLDYTPPMIIGDDAGFSD
                     PAFIPAVGEIAQGAMNRSAWDLGKPGGNSYTVNELFKKKTGRDLDDTSARWMQGFLVL
                     ADAINRAGSTDPAKIQKALQDTDLKPEQLMIGYRGVKFDETGQNTLSATYLIQLQGKE
                     YKSVWPQDRATAKLVWPMTGWRTRNQ"
     gene            2071..>3410
                     /locus_tag="VGA15_18765"
     CDS             2071..>3410
                     /locus_tag="VGA15_18765"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEX9589773.1"
                     /translation="MQRSDIRSQTASVGLAQDQNTETLRWYLLRSGLSLVWSRLESRP
                     ALAFILLSFAFGSAISVVVPPLRGPDEIAHFLRIYSYARGGLLPVTEVDGRKGIFIEH
                     ELYTRLSFFKNAGERFGQNRQQGLRYGAIMQEYSRPAGTLHEQEHAAKFMPFAGTEGY
                     NPAAYAPYVVAAAIGNLLGLDFPNLLLLMRFLGLMTFTAVAAYAIKLTPTLKWAFVLI
                     AMLPVSIYNRSVLSADGAALAYALVITALCFSAVQRCGRIWERSLWMTLCALSKQPQI
                     VFVALELMVCRMTELRRRWSSLALIVVPSFILSPLWVVAVSADIAVWRLAQAETHPRE
                     QFDPLWKLAYMWEHPLHFPLAAWTSVTVWGDRLWPELIGILGWQDVLLPPWTYFVLTA
                     ILLLVPLQKLNLDGAARARIAVMTGLGVLGYIVTVYLIFFLTYTPIDIDHVRGV"
BASE COUNT          565 a         1060 c         1091 g          694 t
ORIGIN      
        1 cgcggcccac ctctccccac ggggagaggt ggagaccgct cagccgagcg aggcgatgat
       61 ctcgcggtag gccgcctcgg gaaacgcctt gagggtgcgg gtgcgcacgt tgcccatgat
      121 gccgagctgc agcgcgaatc tcgccgccac cgcgtcgtcg gaggcttcgc acacgaggac
      181 caggtcgtat tcgcccatgg tcaggtgcac ggatttgatc tcgccgccca tttccttgag
      241 catcttcttc gccgcatcga agcggcgcgg ggaatccttg acctgttgaa tgccccgctc
      301 ggtccaattg acgagcacga tgtaggtggt catggcgcgc ctccgtcgca tggatcagag
      361 gtcagaaatc agaaatcaga aatcgaaacc ttctgacaac tgatatctga aaaacctccc
      421 ctgatccgac tactgacttc tgatcactga ttcctggtcc tccagccggt catcggccag
      481 acgagcttgg cggtcgcgcg gtcttgcggc cacaccgact tgtattcctt gccctggagc
      541 tggatcaaat aggtggcgga cagcgtgttc tggccggtct cgtcgaattt cacgccgcga
      601 tagccgatca tgagctgctc gggcttgaga tcggtgtcct gcagcgcctt ctggatcttt
      661 gcgggatcgg tcgagccggc gcgattgatg gcgtcggcca gcaccaagaa tccctgcatc
      721 cagcgggcgc tggtgtcgtc gagatcgcgg ccggtcttct tcttgaacag ctcgttgacc
      781 gtgtagctgt tgccgccggg cttgccgaga tcccaggcgc tgcggttcat cgcgccctgg
      841 gcgatctcgc cgaccgcggg aatgaacgcc gggtcggaga agccggcatc gtcgccgatg
      901 atcatcggcg gggtatagtc gagattcttc agcgtcttga aatacaggat ggtgtctgcg
      961 gtgtagctga tgaagatgac cgcgtccggc gcgagctgct tgagctgcag cacctggccg
     1021 gagacgtccg agccgttggc gttgtagggg atctgcgccg ccaccttgat gccgctcttg
     1081 ttggccgcgt cgctgatgct ggcggcgacc gaggtgccgt agtcggtgtt ctcgttgacg
     1141 atggcgatgg cgtcgacctt cctgccggct tgtttgagct cgccgagaaa cgaggtgtag
     1201 gccttggcga aatccggcgc gatcggcccg ctgcgaaaaa tccacttgaa gccgcgctgg
     1261 gtgatgttga gcgcgaccga atccgccacc aggaacggga cgccgtagcg ttcggcaacc
     1321 gcggtcgccg tgagcgccac cgaggaatga taggcgccga gcatggcggc gaccttctcc
     1381 tgggtgacga ggcgcagcgt ctggctctgg ccgaccgaag gattgccctg gtggtcggca
     1441 ttgagcagct cgactttcgc gccgcccaga ttggcgagcc ccgcggcccc ggcgagcggg
     1501 ctgcccttga gctcgggata gacgccgttg acgatctcgg cgccgagctc gatcgcatcc
     1561 ttggcggaat tgccggcgct cgccgcattg ccgctgagcg gatagatcac gccgatcttg
     1621 acggtctcgg cggcgcccgc cggaagcgcg atcgccagcg ccaagagcgc ggcggcgagc
     1681 cgatggcccc gatggttcat gcaatcctcc ccgttgcgag cggcattggg tcccgctctt
     1741 tgccgcacca tagcacgaag gcggcgccgt cagcgcgccg ctggcgcaaa ttgacttgcc
     1801 tttccgtcac gatcaagcgc ggacgagcgc aacgcgtccg ctcctcgacc gggaggcttg
     1861 ccgcacgcat cggcagcagc tcgatcgtgg gcgcgaggcc cccgaccgag ctcgccgaac
     1921 ccggccgtcg tgtgccggga tcggcctcgg tcgacgcttc ctcgaggggg gaccactcgg
     1981 acacaggaac gatttccagc aagcaacgtt gagttcatcg gtcagcggtt ggccaggatg
     2041 tgcgcccggg cgtaggcaga gcgagtccat atgcagagaa gcgacattcg ctcgcagacc
     2101 gcctcagttg ggctggcgca agatcaaaac accgagacac tccgctggta cctgctccgg
     2161 agcgggcttt ccctggtttg gagccgcctt gaatcccggc cggctttggc tttcatcttg
     2221 ctctcgtttg catttgggtc agccatcagc gttgtcgttc cgccgcttcg tggcccggac
     2281 gagatagctc acttcctgcg catttactca tacgcccgcg gggggctgct acccgtcacg
     2341 gaggtcgatg gccgcaaggg aatcttcatc gagcacgagc tgtacacccg gctctcgttc
     2401 ttcaagaatg ccggggaacg ctttggccaa aacagacaac agggccttcg gtacggcgcg
     2461 ataatgcagg agtactcgcg cccggccgga acgctccatg agcaggagca cgccgcgaaa
     2521 ttcatgccct tcgcgggcac cgaaggctac aacccggccg cctacgctcc ctacgttgtg
     2581 gcggcagcca ttggaaatct gctcgggctc gattttccga acctgctgct tctgatgcgc
     2641 ttcttgggcc tgatgacgtt cacggccgtg gcggcgtacg ccatcaagct caccccgacg
     2701 ctgaaatggg cattcgtgct catcgcgatg ctaccagtat cgatttacaa tcgatcggtg
     2761 ctcagcgccg atggtgccgc gctggcttac gcgctagtga tcaccgccct ttgtttcagt
     2821 gccgtccagc gatgcggtcg gatctgggag cgctcgctgt ggatgacgct gtgcgcgctc
     2881 agcaagcagc cgcagatcgt gttcgtggcg ttggagttga tggtgtgccg gatgacggag
     2941 ctgcggcggc gatggagcag cctggcgctc atcgtggtcc ccagcttcat tttgtcgccg
     3001 ctatgggtag tagcggtatc cgccgacatc gcggtgtggc gtcttgccca ggcagaaacc
     3061 catccacgcg agcaattcga tccgctgtgg aagctcgcgt acatgtggga acaccccctc
     3121 cacttcccgc tggcagcatg gacgtccgtg accgtatggg gagaccggct gtggccggag
     3181 cttatcggca tcttgggatg gcaggatgtt ctgcttccgc cctggactta tttcgtgctc
     3241 acggccattc tcctcctcgt gcctttgcaa aaactgaatt tggacggtgc cgcgcgtgca
     3301 cgcatcgcgg tcatgacggg ccttggtgtc ctgggctaca tcgtgacggt gtatctgatt
     3361 ttctttctca cctatacgcc gatcgatatc gaccacgtcc ggggcgtcca
//
LOCUS       DASWGM010000667         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1025245,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000667 DASWGM010000000
VERSION     DASWGM010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025245"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..>2740
                     /locus_tag="VGA15_18770"
     CDS             68..>2740
                     /locus_tag="VGA15_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589774.1"
                     /translation="MPAVLTHKTIMLLARERLADIRNVLTRKTAAAGATVSDLEYRML
                     YLATKAYDLMSDHEAGSPDIDFPIGTLYPSPLGKGVSRFAVMGSMGPDITGFSAILAP
                     GNSWVFDTVHKGNPDQNREPVVARTSDFALEFWRQVSTAIDSRAGAANADKQKAQAQM
                     RAYVMGHLCHIVGDVISHPFINDIEWHLRVGSHDKFSHAAGEGSLDAKVARQLLLRPG
                     TREGQAWAAWWPVLDSVPAEFFTAYDATLEQIYTARTKRPTGFGAFEEQFAKLSPPAL
                     NSSFVRDGYKVYRNAILPMGYGWGYWHWWAFLMPIALPLMALFPLSLALPKGSDFLHH
                     PLSQVQGDRPFSEVLTLPLALGSIIPVFYGIWIASITNRGVEALTTGGIITAVVSFIS
                     GIVYLATLGVDNLPWWLRWLVYFAIPVAAGVVFSITAIANGGHGEGGQRALSLIYASP
                     FLIAVIFGALYFAAVGIGAAASSTTAADVTFGILTGIFIIAVGVFWFVLPKKMRDARI
                     PEQAEPFPAERPHFVRLFDDTTLFHDPAQPGNAPLARRFYPSARRPLLKLWWEGAGDL
                     YIRSRRLWLEFSFSGSDAPNQIVPAPIAPMTAVEFAHFLTATVKEHGGKLKAAMVYPT
                     DLDYELPPGATFADEGDPDEENIPSDFSAAKHDELAQNYHKLGTTQASTDYILYHAPK
                     AMQAIRFGRNGPAPFDPREVGAPGLGRIMSDGIHVIGDGSAAFRSFFIPGDQINAKGQ
                     TRIVTLVDSNSRLTLSSAFNSDLPAGTEYSRVGPDREPAEGYTYVANPPAMTPGGESI
                     MDYAADVAAMLCLGATPHLLSTAELTVPSLAGKNDAGGHAVDQKIKKVYQVFRNWNLD
                     RRRVNEWRMLVAGGATSEKGEAADGYDSAMTQPRD"
BASE COUNT          486 a          858 c          859 g          537 t
ORIGIN      
        1 ggtgtgggtg cggcaattgg tgcggcgatc tcggtgggag tttcactctg agagtgggag
       61 gcgacagatg ccggcggtct tgacgcataa gacgatcatg ttgctcgcgc gcgagcgcct
      121 tgcggatatc aggaacgtcc tgacgcgcaa gaccgcggcg gcgggcgcca ctgtcagcga
      181 cctcgagtat cggatgctgt accttgccac taaggcctat gacctgatga gcgaccacga
      241 ggctgggtcg cccgatatcg actttcccat aggcaccctc tatccgtcgc cgctgggcaa
      301 gggcgtttcg cgtttcgccg taatgggttc gatgggtcct gacataacgg gattctccgc
      361 gatcctggcg cccggcaact cgtgggtgtt cgacaccgta cacaagggca atccggatca
      421 gaaccgcgag ccggtggtcg cgcgcacaag cgatttcgcg ctcgagttct ggcgccaggt
      481 ctcaaccgcg atagattcgc gcgccggcgc cgccaacgcc gacaagcaaa aggcccaggc
      541 gcagatgcgc gcctacgtta tgggccattt gtgccatatc gtgggcgatg tcatttcgca
      601 tcccttcatt aacgacatcg aatggcattt gcgcgtcggc tcgcacgaca aattcagcca
      661 tgcggccggc gagggctcgc tcgacgccaa ggtcgcgcgc cagttgctgc tgcggccggg
      721 cacacgcgag gggcaggcgt gggcggcatg gtggccggtg ctggatagcg tgccggcgga
      781 gttcttcacc gcctacgacg caacgcttga gcagatctat accgcgcgca ccaagcggcc
      841 gacggggttc ggtgcgttcg aagaacaatt cgccaagttg tcgccgcccg cgctcaactc
      901 aagcttcgtg cgcgacggct acaaggtgta ccgcaacgcg atcctgccga tgggctacgg
      961 atggggttac tggcactggt gggctttcct gatgcctatc gcattgccgc tgatggctct
     1021 gtttccgctg tcgctcgcat tacccaaagg cagcgacttt ctccaccatc cgctgtctca
     1081 ggtgcaggga gaccggcctt tctccgaggt gctgacgttg ccgctggcgc tgggctcgat
     1141 cattccggtg ttctacggca tctggatcgc gtcgatcacc aaccgcggcg tcgaagcact
     1201 gacgacaggc ggaatcataa ctgctgtggt ctccttcatc tcgggaattg tctatctcgc
     1261 aacgttgggc gtggacaatt tgccctggtg gttgcgatgg ctggtctatt tcgcgattcc
     1321 ggtcgccgcg ggagtcgtct ttagcatcac cgcgatcgcc aacggcggcc atggcgaagg
     1381 cgggcagcgc gccttgtccc ttatttacgc gtcgccgttc ctgatcgcgg tgattttcgg
     1441 tgcgctttac tttgcggcgg tcggaatcgg cgcggcggcc tcctctacaa cggcggctga
     1501 cgttaccttc ggcatcctca ccgggatctt cataattgcg gtcggggtgt tctggttcgt
     1561 gctgcccaag aagatgcgcg acgcgcgtat tccagagcag gctgagccct ttccggccga
     1621 gcggccgcat ttcgtacgcc tgttcgacga cacgacactg ttccacgacc cggcgcaacc
     1681 gggtaacgcg ccgctggcgc gccgcttcta tccctccgcg cggcgtccgc tgctcaagct
     1741 gtggtgggaa ggagcgggcg atctctacat ccgctcacgg cgcttgtggc tggagttcag
     1801 cttcagcggc agtgacgctc ccaatcagat cgttcccgcg ccgatcgcgc cgatgaccgc
     1861 ggtcgaattc gctcatttcc taaccgcaac cgtcaaggag cacggtggca agctcaaggc
     1921 agctatggtc tacccgacgg acctggatta tgagcttccc cccggggcga ccttcgccga
     1981 cgagggcgat cccgacgaag agaacattcc gtcagacttc agcgccgcca agcacgacga
     2041 actggcccag aactatcaca aactgggtac cacccaggcc tcgaccgatt acatccttta
     2101 ccatgcaccc aaagcgatgc aggcgattcg gttcgggcgc aacgggccgg cgccgtttga
     2161 cccgcgcgaa gttggagcgc ccggccttgg ccgcatcatg agcgacggaa tccatgtgat
     2221 cggcgacggc tcggccgcgt tccgcagctt ctttatcccc ggtgaccaga tcaacgccaa
     2281 gggccagacg cggatcgtga cgctggtgga ttccaacagc aggctcaccc tgtcatctgc
     2341 gttcaattct gacttgcctg ccggcaccga atacagcaga gtcggcccgg atcgcgagcc
     2401 cgccgaaggc tacacctacg tcgctaaccc tcccgccatg acgccgggcg gcgagagcat
     2461 catggattat gcggccgacg tcgccgcgat gctttgtttg ggcgccacgc ctcatttgtt
     2521 gagcaccgcg gaacttacgg tgccgtcgct ggcggggaag aacgacgctg gcggtcacgc
     2581 ggtggaccag aagattaaga aagtgtacca agtgtttcgc aattggaatc tcgaccggcg
     2641 ccgcgtcaac gaatggcgca tgctggtcgc gggcggtgca accagcgaaa aaggcgaggc
     2701 ggccgatggt tacgactcgg cgatgaccca gccgcgcgac
//
LOCUS       DASWGM010000668         4731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2581872,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000668 DASWGM010000000
VERSION     DASWGM010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2581872"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VGA15_18775"
     CDS             complement(<1..814)
                     /locus_tag="VGA15_18775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9589775.1"
                     /translation="MSSRGLVWSGSVLAVLAVAAVGGAYWKYPDFFQPPTVTTGVAVL
                     GPVSEAVYGTGTVEPERWAKVVPLQRRRLIELCRCEGQAVKAGQVLGRQDDAEEVSAL
                     HELEINNEQLTRDLDRASRDRDKSEAAKTEYEQRSTQFEQSKSRISAQKTRIDSLVLR
                     APLDGMVLRRDGEVGEIVGPTEVLFWVGPPTPMQVVAEINEEEITRIAAGQKAFLRSE
                     AFSAQALRATVSQITPKGDPTRKTFRVYLRLPPDTPLRIGMTVEVNIIYREKT"
     gene            complement(837..1526)
                     /locus_tag="VGA15_18780"
     CDS             complement(837..1526)
                     /locus_tag="VGA15_18780"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9589776.1"
                     /translation="MKRVFLAMLCGAALIASSVAYAGDAVSDKEMADRYSRAALSGDS
                     DAQFYLAALYSSGVGRPRSDEEAFRWFSRAADQGHSHAMLVLSGIYAVGRGVSKDNFN
                     AYKWAYIVAVGSRVDEFRNGSRQLMGVLETRMTRDDVDRAKAEAGRWHATSSQPKPAA
                     APAPADSLARDYQSPQLSSAVSKQPPATTSNNSTVTGSLPGPRNTSEKKNDMDALLDR
                     VPSGLRKRFGF"
     gene            1739..2044
                     /locus_tag="VGA15_18785"
     CDS             1739..2044
                     /locus_tag="VGA15_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589777.1"
                     /translation="MVVATPVAASTFDGAWNVQIASANAACGGGATISIGINNGQVES
                     SNALMKATGRVADAGGISVTLISGIKRAVGSGHLSATSGSGTWRGALCSGTWTAQRI"
     gene            complement(2117..2521)
                     /locus_tag="VGA15_18790"
     CDS             complement(2117..2521)
                     /locus_tag="VGA15_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9589778.1"
                     /translation="MNIHLAGSRPTRLAPKEYFTGTVWQDPIIAAEVPARIVSNRVSF
                     EPGARTNWHSHPLGQTLYVISGVGRVQSKGGPIREIKAGDVVWIPPGEKHWHGGSPTN
                     GMIHIAMQESLDGNHATWMEPVTDAEYATPVG"
     gene            complement(2564..3262)
                     /locus_tag="VGA15_18795"
     CDS             complement(2564..3262)
                     /locus_tag="VGA15_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HEX9589779.1"
                     /translation="MSLTPEAIKTLSHVSTATITTVLLKKGLRNVWLRGSRPLRPGQK
                     RLVGPAFTLRFVPAREDLATPESWSSPISTRTAIEAMPEGCIAVVDAMGITDAGIFGD
                     ILCARMIKRGVAALVTDGVVRDLEGVLGTSLPVWCDGFAAPPSVAGLTFVGWGEPIGC
                     GGVAIFPDDIIVADQDGTVVIPKAFLDLILAEGAEQERMEAWIVEQVNGGAVLPGLYP
                     MNAETKARYAASKK"
     gene            3500..4231
                     /gene="pxpB"
                     /locus_tag="VGA15_18800"
     CDS             3500..4231
                     /gene="pxpB"
                     /locus_tag="VGA15_18800"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEX9589780.1"
                     /translation="MAATLSPPRLLPSGDSAITVEFSRSIDEMANQRVLALDRTLARE
                     TITGVTETVPTYRSLLVHYDPVKIDFDTLSGKLVTLAQLPVPPVTKMRRWRIPVAYGG
                     EYGIDLEDVARTLKSTPENIVARHVAGDYRVAMIGFTPGWSYLSGLDASLQMPRLHNP
                     RLVTPAGTVSIGGVQTGVQCLAGPSGWHLLGRTAVRTYQLHRDPIFLLEPGDAVTFSA
                     VDAKTFAEQDRAAAAGEIVAELMTS"
     gene            4228..>4731
                     /locus_tag="VGA15_18805"
     CDS             4228..>4731
                     /locus_tag="VGA15_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEX9589781.1"
                     /translation="MSKLVISAIGPASSVQDGGRYGAQRYGLTPSGAMDRLALATANS
                     LVGNAAFAAAIEIGPFGAKFTARQGAVRIALAGASRNADISGRAVASNTSTTIADGEI
                     LTLGFARGGSFSYLAIEAGIAGEPMFGSLAVNARAGLGSPYPRPLQAGDELQTAAASG
                     AAENRIDL"
BASE COUNT          882 a         1508 c         1446 g          895 t
ORIGIN      
        1 cggtcttttc gcgatagatg atgttgactt cgaccgtcat tccgatccgc aatggcgtat
       61 cgggtggcaa tcgcagatag acacggaagg tctttcgcgt gggatcgccc tttggcgtga
      121 tttgcgaaac cgtggcccgc aacgcctgcg cggaaaacgc ctcgcttcgc aaaaaggcct
      181 tctgccccgc ggcgatcctg gtgatttcct cctcgtttat ttcggccacc acctgcatcg
      241 gcgtcggcgg gccgacccag aacagcacct cggttggtcc gacaatctcg ccgacctcgc
      301 cgtcgcgacg caacaccatg ccatcgagcg gcgcgcgaag caccagcgaa tcgatgcggg
      361 tcttttgggc gctaatccgc gatttggatt gctcaaattg agtagagcgc tgctcgtatt
      421 cggtcttcgc ggcttcgctc ttgtcacggt cacggctcgc gcgatccaga tcgcgcgtga
      481 gttgttcgtt gttgatttcc agttcatgga gcgcgctgac ctcttcggcg tcgtcctggc
      541 ggccgaggac ttgaccggct ttgacggcct gcccttcgca gcggcagagt tcgatcaacc
      601 gacgcctttg cagcggcacg actttggccc agcgctccgg ctccacggtt ccggtgccat
      661 agacggcctc tgaaacgggc ccgagaacgg ccactcccgt tgttacggtc ggaggttgaa
      721 agaagtccgg atacttccaa taggccccgc caacggcggc aacggcaagc actgcgagca
      781 ctgatccaga ccagacaaga ccgcgcgaag acatcggctt ctcctaaaat ataatatcaa
      841 aagccgaacc gcttgcgcag tccggagggc actcgatcca atagcgcgtc catatcgttc
      901 ttcttttcgc tggtgttgcg cggccctgga agactgccgg taacggtgga attgttactc
      961 gttgtcgcgg gcggctgttt cgaaacagcc gaggatagtt gcggcgactg gtagtcgcgg
     1021 gcgagactgt cggccggagc cggagcagcg gcaggcttcg gctggctcga tgtcgcgtgc
     1081 cagcggccgg cttcggcctt ggcccggtcg acatcgtcgc gcgtcatcct tgtttccaga
     1141 acgcccatga gctggcgact tccgttgcgg aattcgtcaa cccggctgcc aaccgcgaca
     1201 atgtaggccc acttataagc gttaaagttg tctttcgaaa ctcctcgccc aacggcatag
     1261 atgccgctga gaaccagcat ggcatgcgaa tgaccctggt cagcggcgcg cgaaaaccat
     1321 cggaaggcct cttcatccga cctcgggcgc ccgacgcctg atgagtaaag cgcagcaaga
     1381 tagaattgag cgtcgctatc tcctgacagc gccgcccgtg aatagcggtc ggccatttcc
     1441 ttgtcggata ccgcatcccc ggcataggca acggagctcg cgatgagagc ggctccgcaa
     1501 agcattgcta gaaaaactcg cttcatggca atccccgtcc aaaataaccc agcctgcact
     1561 atttcgctga tgttacccgc gatcgtttct tagcccaagg ccttatctcc tcatgtgatt
     1621 tctctcacag tctccgacga accgatctga gaggttgatt gcgccggaac caagggagca
     1681 tgtcatggga tacttttgca aaacattcgt cgccacccgc atcctggtac tgctgggaat
     1741 ggtggtcgcc acgcccgttg cggcctcgac atttgacggt gcatggaacg tccagattgc
     1801 ctcagcgaac gccgcatgcg gcggcggggc aaccatctcg atcggaatta acaatggcca
     1861 ggtcgaatcc agcaacgcat tgatgaaggc gacgggccgg gtcgccgatg ccggcggcat
     1921 cagcgtaacc ctgataagcg gaatcaagcg ggccgtcggg tcaggtcacc tcagcgcgac
     1981 gtcaggatcg ggtacctggc gcggcgcact gtgctcgggt acctggacag cgcagcgtat
     2041 ctagccgcac cgtatggcgt catccgaagc cacgaaccac tgtggcgaca ctcgaaggat
     2101 gacgaccgac ctctcgtcaa cctacagggg tggcatattc cgcatcggtc accggctcca
     2161 tccaggtcgc atgatttccg tccagcgatt cctgcatcgc gatgtggatc atgccgttgg
     2221 tcggcgagcc gccgtgccaa tgcttttcac ccggcggaat ccagacgaca tcgccggctt
     2281 tgatttcgcg tatcggcccg cctttgctct ggacccggcc gaccccggag atcacgtaca
     2341 gcgtctggcc gagggggtgc gaatgccaat tcgtccgcgc gcccggctca aaggatacgc
     2401 ggttcgagac aatccgcgcc ggtacctcag ccgcaatgat cggatcctgc cagaccgtac
     2461 cggtaaaata ttctttgggg gcgaggcgtg tcggccgcga accggcgaga tggatattca
     2521 tggcattcct ccttcttatg cagacgcttc cggtacgtcc cggttatttc ttcgaagccg
     2581 cgtagcgcgc cttggtctcg gcattcatgg gatagagacc gggcaacacg gcgccgccgt
     2641 tcacctgttc gacgatccag gcttccattc gctcctgctc cgcgccttcg gccagaatga
     2701 ggtcgagaaa cgctttcgga atcaccacgg tcccgtcctg atcggcgacg ataatatcgt
     2761 ccgggaagat cgccacgccg ccgcagccga tcggctcgcc ccaaccgacg aatgtcagtc
     2821 cggccaccga cggcggggcg gcaaagccgt cgcaccacac cggcaagctg gtgccgagca
     2881 cgccttcgag atcgcgcacg acgccgtcgg tcaccagcgc agccaccccg cgcttgatca
     2941 tgcgcgcgca cagaatatcg ccgaagatgc cggcgtcggt gatgcccatg gcgtcgacca
     3001 cggcgatgca gccttcgggc atggcttcga tcgcggtccg ggtcgaaatc ggcgatgacc
     3061 atgattccgg cgtggccaga tcctcccgcg cgggcacgaa acgcaacgta aaggccggtc
     3121 cgaccagccg cttctgtccc ggccgcagcg ggcgcgagcc gcgcagccag acattccgca
     3181 agcccttctt gagcagaacc gtagtgatgg tggcggtgga cacgtgcgag agggtcttga
     3241 tcgcttcggg ggtcagggac atcgaaaacg tagctccggt tgcggatgat caggcgggcg
     3301 catcttgcgg ggcaagcgca tcgcgtcaag tgtcgggaac tcacggctga aggcgcggca
     3361 tcgcatggcg cgatcaccgg gtcttatcac acgtatgcag attaatttat tgaacttgct
     3421 ggccgatttg cgtgaagcgg attccacttc gcggcaaaac aggctagagc tggatatccg
     3481 ccgacgagtt tgtgaggtta tggccgcgac actatccccg ccccgcctgt tgccgagtgg
     3541 cgacagcgcc atcacggttg aattcagccg cagcatcgat gaaatggcga accagcgggt
     3601 gctggcgctc gatcgcaccc ttgcacgcga gaccatcacc ggcgtaaccg agaccgtgcc
     3661 gacctaccga tccctgctgg tgcattatga cccggtcaag atcgacttcg acacgctgag
     3721 tgggaagctc gtcaccctcg cgcagttgcc ggtaccgccg gtcacgaaaa tgcggcgctg
     3781 gcgcattccc gtggcttatg gcggcgagta cggcattgac ctcgaagacg tcgccaggac
     3841 actcaagtcg acgcccgaaa acatcgtcgc gcggcatgtc gccggcgact accgcgtcgc
     3901 gatgatcggc tttacgccgg gctggtccta tctcagcggc cttgacgcct cgctgcagat
     3961 gccgcggctg cataatccgc ggctggtgac acccgcgggc accgtctcga ttggcggcgt
     4021 gcaaaccggc gtgcaatgtc tcgccggtcc gagcggctgg catctgctgg ggcggaccgc
     4081 ggtgcgaacc taccagctgc atcgcgatcc gattttcctg ctggaacccg gcgatgccgt
     4141 gacgttctcc gcggtcgacg ccaagacttt cgccgagcag gatcgcgccg cggcagccgg
     4201 cgagatcgtt gccgagttga tgacctcatg agcaaactcg ttatttcggc gattggcccg
     4261 gccagctcgg tgcaggacgg cggccgttac ggcgcccagc gttatggcct gacgccgagc
     4321 ggcgcgatgg accggctcgc gctcgcgacg gcgaatagtc tggtcggaaa cgccgccttc
     4381 gccgccgcaa ttgaaatcgg cccgttcggc gccaaattca cggcgcggca gggtgccgtt
     4441 cgcatcgcgc tcgccggcgc atcgcgcaac gccgacatct cgggacgcgc ggttgcatcg
     4501 aatacatcca cgaccatagc cgacggcgaa atcctgacgc tgggcttcgc ccgcggcggc
     4561 tctttcagct acctcgcgat cgaagccggg attgcgggcg aaccgatgtt cggcagcctg
     4621 gcagtgaacg cgcgcgcggg gctcggcagc ccgtatccac gcccgctgca ggccggcgac
     4681 gaattgcaga cggcggccgc aagcggcgcg gcggagaacc ggatcgatct t
//
LOCUS       DASWGM010000669        11355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2686030,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000669 DASWGM010000000
VERSION     DASWGM010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686030"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1340..2584
                     /locus_tag="VGA15_18810"
     CDS             1340..2584
                     /locus_tag="VGA15_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589782.1"
                     /translation="MTTQQPKILLSEGSSLSSREAITALGLAGHRVELVSSTPMSLGR
                     FSRFVSRVHRAPASGADPDGYLAAVTDIVGAENIDAVIPVHEQAYLFAAARQKLPAGV
                     GIALADFAAFEQVQSKARLATLLTQLKVTQPDTEIIRSADDFAANRPYPFFVKAAFGT
                     ASAGVWRVGDAHARDALLLGLEAHDAFAQGLLIQAAVTGSLERTQGVFDRGRLVASHI
                     YRQVVEGPGGGDVLKISVVSAGVRDTVERIGQALQWHGALSFDYIRDDATNTPHFIDA
                     NPRLVEPMNAWLSGVDLPGALLRVSLGETPPVQPDGREGVLTRLGLMGLLDAARRRNR
                     RRDILREIGLLAFGMGRYRGSREELVPLLTDPWCAIPLGVVVARLLRAPETAARFSET
                     TVAAYSLTPHAIQRLRTWHDAA"
     gene            complement(2599..3453)
                     /locus_tag="VGA15_18815"
     CDS             complement(2599..3453)
                     /locus_tag="VGA15_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9589783.1"
                     /translation="MTSAGAKRHRERLGERLRDVRTAAWKPRYRLARYAVSICLLALA
                     SAAADARSLEIITERGALTLCANPNALPFASKTGPLPGFQIELGEKIAQQLGVKLTRE
                     WVISAIQYRRADCDLVLDTIARKDTPPAGGVQVSRPYHRSGVVLAVRADSQASSLASL
                     GSDQRVGVPVGSLVSMTLGKGGTVTSPFVFEDDILAALANREIEAAAVTPATVGWFNL
                     QHADKPLRLIPAFDNDSDLNWNIAAGLFRPDDKLRQRVDAAIEALLADGTIARIYSRY
                     GIELRPPR"
     gene            complement(3446..3805)
                     /locus_tag="VGA15_18820"
     CDS             complement(3446..3805)
                     /locus_tag="VGA15_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9589784.1"
                     /translation="MRILFVIVASMAIIWPCLSTAAVAEQSTSAGSASPPAQPEASSS
                     DIDGEQMFATTCGFCHQDGGRAAGRGPKLSKTERSDEFIIERIKKGKMGSMPAFGSVF
                     SDGQIIAILAYIRGLDD"
     gene            complement(3873..5597)
                     /locus_tag="VGA15_18825"
     CDS             complement(3873..5597)
                     /locus_tag="VGA15_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9589785.1"
                     /translation="MALYYARYLGGCAALALAAVLTASPTHAQDAAALNAEAAQNDWS
                     THHGTYKSYHYSGLEQINADNVKNLEVAWTHYPERATRGLQSTPLAMDGVLYYSGSYS
                     RVYALDGATGKTIWTYAPELDEELVAKQTHSPYNRGIALGQGNLYVGTVDGRLIALNL
                     KTGKPAWDLKLLDSKKVTVGFTGAPLVVKDMVIIGAQGGEWPGRGPIFGVDGKTGQKK
                     WQFDTVAGTEEAMATWGNDSWRTGGGGGWMPGAYDPETNSVWWGTANPAPLYDWSGAD
                     WKKSGPRPGDNLYTTSVIALDPDTGKLKFYHQELPHDAWDFDSAVGEFVMINRGDKKL
                     VVHANKSGFVFVYDRSNAKVENVWPLVKNINFVKGIDPKTGELIGRRDLVAGKAETPL
                     CPAIAGGISWNSGAYSPKTGLYYKIGQEWCMELTVLKTTPILEPMAQLNIGADFKLVA
                     PPDGAARGHLDARDPVTGVLKWEVNYKQPPLASVLATAGNLVFVPDAEGVVHAYNADS
                     GKELWSHNNGMGHNAGIISYMAGGKQYIAVPAGWGSLVADEFVALYGEPFKSMAKNTG
                     ALVVFALK"
     gene            6306..7547
                     /locus_tag="VGA15_18830"
     CDS             6306..7547
                     /locus_tag="VGA15_18830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9589786.1"
                     /translation="MARLKFGAFLAPHHPIGEHPMLQFRRDLDLVEQLDALGYDEFWC
                     GEHHSSGWETIASPEMFLAAAGERTKRIKLGTGVVSLPYHHPFNVAQRMVQLDHMTGG
                     RAIFGSGPGALASDAHTLGIDPMTQRDRQDEAIAVIRRLFNGERVTARSDWFTMNDAA
                     LQILPLQEEMPFVVASQISPSGMTLAGKYGIGIISLGSMSTQGLMALPTQWGFAEDAA
                     RKHGTTVSRSNWRVLLSWHIAETREQARREAGAGLMRWHNEYNVGTLQRPGLQPFTSP
                     EDAVEKTAGGEAAASTIGTPDDLVKTIKSLMDVSGGVGTIVGFVHDWANPENTRRSWD
                     MVARYVVPEINGYIDGLRKSQKFVIENRAVFERAGQAVMAKIMENDKAAAALPLTGPG
                     RVAIPTINAPDLQKEAAKQKA"
     gene            7782..9161
                     /locus_tag="VGA15_18835"
     CDS             7782..9161
                     /locus_tag="VGA15_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9589787.1"
                     /translation="MSMQSVASPAISFTPPKRNSLTHIPGDEGWPLIGKTLEILADPK
                     GQVERQAARYGLVYRSHIFGETSLSLLGPEANELVLFDQAKQFSSTHGWGRILGLLFP
                     RGLMLLDFEEHRLHRRALSVAFKSGPMKSYLAALDTGIAARVAQWKAQPGPMLFYPAM
                     KQLTLDLAATSFLGAEIGPEVDEITRAFVDMVAASVAVIRKPLPGTAMARGVRGRKRI
                     VAYFSEQIPIRRAKGGGEDLFSQLCHATHEDGALLSTQDIVDHMSFLMMAAHDTLTSS
                     LSSFVGALAAHPQWQDKLRDEVTALGVATGEATTFDNLEAMPLSEMAFKEAMRLKPPV
                     PSIPRRTIRDLTFKGYAIPAGTMVGVNPLFSHHMPDIWPDPERFDPMRFTDEAQRGRH
                     RFAWVPFGGGAHMCLGLHFAYMQAKCFTRHFLQNLTVSLEPGYTPDWQMWPIPKPRDG
                     LRVTLSPVT"
     gene            complement(9174..10208)
                     /locus_tag="VGA15_18840"
     CDS             complement(9174..10208)
                     /locus_tag="VGA15_18840"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9589788.1"
                     /translation="MNARLERKLRSLAALVAAGLIAGMVFDVAQGRTSPTFLAVGASY
                     GLLMSVTLGSTELFVLEGPIREWLGGLSFATRLMVRSTIYVAIITVIQWFQLGEVIAG
                     LSPDTSTFWSSIIYSAVLSVVMNLALGVTNIIGPRAFLNFFIGRYHTPVEEKRFVLFV
                     DIAGSTGLAERLGGIAIHRLLDRTFRLLTVAVVDYRGEVLNYVGDEVIVTWPERGGAI
                     DCRPLRCFVAMRDELSRASGQLEREFGAAPRIRGSLHFGPVIVGEIGDVKRAIVFNGD
                     VMNTAARLEELSRNVDGGFLASRAAMERFSSAPPFAVRDLGRLPIRGRADGIDVVGID
                     AQPLPDLALA"
     gene            complement(10292..10795)
                     /locus_tag="VGA15_18845"
     CDS             complement(10292..10795)
                     /locus_tag="VGA15_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9589789.1"
                     /translation="MSTTAAMMQTRPEARPLYAAIIAGLACAFVIGKTLPIMMDTLST
                     VLAPLCAVGDTSGSTPDTVEPISSHALPNVPGKRVTIVRVFYGPGGFTPAHRHAGSVT
                     AYITKGEIRSQLAGGPVETFKVGQSFFEPPGATHLISANASATETAELIAVFVADEGA
                     QLTTFVE"
     gene            complement(10887..>11355)
                     /locus_tag="VGA15_18850"
     CDS             complement(10887..>11355)
                     /locus_tag="VGA15_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589790.1"
                     /translation="HMRRQGSGSIINNGSIAGRLAGFSSSMVYGAAKAAVIHLTKCVA
                     MELGESGIRVNSISPGAIATGIFGKALGLSVEAAEKTSDVMREVYKTAQPIPRAGLPE
                     DIAHAAVFLASDESSFINGHDLVVDGAITGGRNWTQQQQGYVALRKAFDQGAG"
BASE COUNT         2059 a         3624 c         3568 g         2104 t
ORIGIN      
        1 gggccgcggc tttgctccac gcttccttca gaccccgcct cgcgacgacg cccttgcgct
       61 ttgctaatcc ttcgccacca tcaggctgga taaaggactt tcacctccaa gctgtcgatc
      121 atgctcggca cacagccaac gtgtcgcgcg aatgcgcgcc cgatgataga ctccgcgata
      181 cccatcaatt gcgttttatg tgatcggttt cgcaccgctt aggacgggtg gagcgaagcg
      241 atacccatca gttgcatttt atgtgatggg tttcgcaagg ctcaacccat cctacgcgct
      301 gcaccgcgtc cgggacacga gcggaatttg ctctcgccgc cgtgtggtga tttgcccgtc
      361 gggcggtttg ttgacagggc tgtccagccc tctttgcaaa aatatttcgc ttccgtggtc
      421 ggtagaaaca tctctatgag tctcgtcatc ccgtcccaca cagaggggcg tatcgcgatc
      481 gtcacggacg tagggcacgg gatgcggtgg acgcggcagc gttttgcgcg cgatgggatc
      541 gcagggcggg ttctttgaat ccgtgagcga tcaacagcac gctgacgaga gatgctggtc
      601 gcggacggca aaatcgtgtg gtcctgacgc cccgacgctg gcgtcaagtc cgcggagttg
      661 cgtcggccca accgggctta gacaaaacct atccgctgac gacggtggca aaagagcccg
      721 gtcaccgggg agagcacgac ataagctgtt aaaaccactg cgtgcgggaa tgccgggcgt
      781 tccggtgtac tcgttgttac tcgtgtgcgt tctaccaata caaagtgcac acgagaccgc
      841 gggtgcagtg ggcacccggt attccccacg ccctctaggg ggcggaagat ttcatgcaca
      901 ctcgggcgct tcgcgccgcg aggtcgcgaa gctgcgtctg gtttcgtcgt cattgcgagg
      961 agcgaagcga cgaagcaatc cattctttct tcgcggcgag atggattgct tcgcggagcc
     1021 tgtcatcggg cgcgcattcg cgcgacccgt tggctcgcaa tgacggctct acaacgaatt
     1081 gtctttggct gtttgaagat tgaatcgagg atcagagtgc gcagcactcc tcaacctcag
     1141 tcacacgaaa cccacccgtc atgcccggac ttgatccggg catccatcca tcttcgtaaa
     1201 aagttgtttc gaagaagatg gatcaccgga tcaagcccgg tgatgacgat ctcacggcgc
     1261 agccggaacg cgagcctccc ctgttcgctt tgtattcgcg ctccagccag cctaagctgc
     1321 gcgccgaggc aacgctcgaa tgacaaccca acagccgaaa attcttctga gcgaaggctc
     1381 cagcctgtcg tcgcgcgagg ccatcacggc gctggggctc gccggccacc gggtcgaact
     1441 ggtctccagc actcccatga gcctcgggcg cttctcgcgg ttcgtcagcc gggtccatcg
     1501 tgctcccgcc tccggcgccg atccggatgg ctatctggcc gcggtgaccg acatcgtcgg
     1561 agcggaaaat atcgacgccg tgatcccggt gcatgagcag gcctacctgt tcgccgccgc
     1621 gcgccaaaaa cttccggccg gcgttggcat agcgctggcc gatttcgcgg cgttcgaaca
     1681 ggtccagagc aaggcaaggc ttgcgacgct gctgacgcaa ctgaaggtga cccaacccga
     1741 caccgaaatt attcgatcgg cggatgactt cgcagccaac cggccctatc cgtttttcgt
     1801 caaggccgcc ttcggcaccg ccagcgccgg ggtctggcgg gtgggcgacg cgcacgcgcg
     1861 cgatgcgctg ctgcttgggc tcgaagcgca cgatgcgttc gcgcagggcc tgctgatcca
     1921 ggccgcggtg accgggtcgc tcgaacgcac gcaaggcgtg ttcgatcgcg gccgtctcgt
     1981 cgccagccat atctatcgcc aggtggtgga aggaccgggc ggcggcgacg tgctcaagat
     2041 cagcgtcgtc agcgccgggg ttcgcgacac cgtcgaacgc atcggacaag cgctgcaatg
     2101 gcacggcgcg ttgtcgttcg actacatccg ggacgacgcg accaacacgc cgcatttcat
     2161 cgacgccaat ccgcgcctgg tcgagccgat gaatgcgtgg ttgagcggtg tcgatttgcc
     2221 cggcgccttg ttgcgggtct cgctcggtga gacgccgccg gttcaacccg acggccgcga
     2281 gggtgtcctt acccggcttg gactgatggg actgctggat gccgcgcggc ggcgcaaccg
     2341 caggcgcgac atcctgcgcg agatcggttt gctcgccttt ggcatggggc gatatcgcgg
     2401 ctcgcgcgag gaactggtgc cgctgctgac cgatccgtgg tgcgcgattc ccctcggcgt
     2461 ggttgtggcg aggctgttac gtgctcccga gaccgccgcg cgattttccg agaccacggt
     2521 ggcggcctac agcctgacac cccacgccat ccaacggctg cgcacatggc acgatgccgc
     2581 ataggccgat cgcgcggctc accgcggcgg gcgcagctcg atgccgtagc gggaatagat
     2641 ccgcgcgatg gtgccatccg ccagcagcgc ctcgatcgcc gcatcgacac gctgtcgcag
     2701 tttgtcgtcg gggcggaaca gcccggccgc gatgttccag ttcaaatccg aatcgttgtc
     2761 gaacgccgga atcaggcgca acggcttgtc ggcatgctgc aggttgaacc agccgaccgt
     2821 cgccggcgtg accgcggccg cctcgatctc gcgattggcc agcgcggcaa ggatatcgtc
     2881 ctcgaacacg aacggcgacg tgacggtgcc gcccttgccc agcgtcatgg agaccagcga
     2941 gccgaccggc accccaaccc gctgatcgga gccgagactg gccagcgacg acgcctgcga
     3001 gtcggcccgg accgcgagca cgacgccgct gcgatggtag ggacgcgata cctgcacgcc
     3061 gcccgcgggt ggagtgtcct tgcgggcgat ggtgtcgagc acgaggtcgc agtcggcgcg
     3121 gcgatactgg atcgcactga tcacccactc ccgcgtcagc ttgacaccga gctgctgcgc
     3181 aatcttctcg ccgagctcga tctgaaatcc cggcagcggt ccggtcttgc tggcgaacgg
     3241 cagcgcgttg ggattggcgc agagcgtcaa cgccccgcgc tcggtgatga tttcgagcga
     3301 gcgggcatct gccgcagcac tcgcgagcgc cagcaggcag atgctgactg cgtatcgggc
     3361 gaggcggtat cgcggcttcc acgccgcggt cctgacatcg cgaagtcgtt cgccaagtcg
     3421 ttcgcgatgt cgtttcgcac ctgcgctagt catcgagccc ccgaatgtag gccaggatcg
     3481 cgatgatctg gccgtcgctg aacactgagc caaacgcggg catggagccc atcttgccct
     3541 tcttgatgcg ctcgatgatg aattcgtcgc tgcgctcggt cttcgacagt ttcggacccc
     3601 tgcccgcggc gcggccgccg tcctggtggc aaaagccgca ggtggtggcg aacatttgct
     3661 cgccatcgat gtcgcttgac gaggcctcgg gttgcgccgg cggacttgca gatcccgccg
     3721 aggtcgactg ttcggcgacg gcggcggtcg acagacatgg ccagatgatg gccatggagg
     3781 caacaatcac aaacaggatt cgcattccgg catccgtgtt gaccggcaaa ggcggcaagg
     3841 aaaaatcctt gccgccaacg ccgtcgtcgc tgtcatttca gcgcgaagac aaccagggcg
     3901 ccggtattct tcgccatgct cttgaagggc tcgccataga gcgcgacgaa ctcgtcggcg
     3961 accaggctgc cccagcccgc cggtaccgcg atgtactgct tgccacctgc catgtagctg
     4021 ataatgccag cattatggcc catgccgttg ttgtgcgacc agagctcctt gccgctatct
     4081 gcattgtagg cgtgcacgac accttcggca tccggcacga acaccaggtt gcccgcggtg
     4141 gcgaggacgc tggcgagcgg cggctgcttg tagttgacct cccacttcaa gacgccggtg
     4201 acgggatcgc gggcgtcgag atgaccacgg gcggccccgt ccggcggcgc caccagcttg
     4261 aagtcggcgc cgatgttgag ctgggccatc ggctcgagaa tcggcgtggt cttcagcacc
     4321 gtaagctcca tgcaccactc ctggccgatc ttgtagtaga gcccggtctt cgggctgtag
     4381 gcgccggagt tccagctgat gcccccggcg atcgccgggc acaacggagt ttccgccttg
     4441 ccagccacga ggtcgcggcg accgatcaat tcgccggtct tcggatcaat gcccttgacg
     4501 aagttgatgt tcttcaccag cggccagacg ttctcgacct tggcgttgga gcgatcatag
     4561 acgaagacga atccgctctt gttggcgtgc accacgagct tcttgtcgcc acggttgatc
     4621 atgacgaact cgcccaccgc ggaatcgaag tcccaggcat cgtgcggcaa ctcctggtga
     4681 tagaatttga gcttgccggt atcgggatcg agcgcgatga ccgaggtggt atagagattg
     4741 tcgcccggcc gcggtccgct cttcttccag tcggcgcccg accagtcgta aagcggcgcc
     4801 ggattggcgg taccccacca caccgaattg gtctccgggt cataggctcc gggcatccag
     4861 ccgccgccgc cgccggtccg ccaggagtca ttgccccagg tcgccatcgc ctcttcggtg
     4921 ccggcgaccg tgtcgaactg ccatttcttc tgtccggtct tgccgtcgac accgaagatc
     4981 ggtccgcgcc cgggccattc gccaccctgg gcaccaatga tcaccatgtc cttgaccacc
     5041 agcggggcgc cggtgaagcc gaccgtcacc ttcttggaat ccaggagctt cagatcccac
     5101 gccggcttgc cggtcttcag gttgagcgcg atcaggcgcc catcgaccgt accgacataa
     5161 agattgccct gtccgagtgc gatgccgcgg ttgtacggcg agtgcgtctg cttggcgacc
     5221 agttcctcgt cgagctccgg ggcataggtc cagatcgtct tgccggtcgc gccgtccagc
     5281 gcgtagaccc ggctgtagga gcccgaatag tacagcacgc cgtccatcgc gagcggcgtc
     5341 gactgcagac cgcgggtggc gcgctccgga taatgcgtcc acgcgacttc gagattcttg
     5401 acgttatcgg cattgatctg ctcgaggccg ctgtaatggt aggacttgta ggtgccgtga
     5461 tgcgtcgacc agtcgttctg ggccgcctcg gcattgagcg cggccgcgtc ctgggcgtgc
     5521 gtcggcgagg ccgtcaatac ggctgccagc gccagcgcgg cgcagcctcc gagatatcgt
     5581 gcataataaa gagccatctg tatcctcctg tgctgtacgt ttgttggacc gcgttgtcgt
     5641 cgcgaccttt gcaatgttct gatgtcaacg cgtcatcggg ccaagggtct cgacatagat
     5701 cgcatggacc gacggaagtg ccgtcaggaa gacacggctg cacttctcgc cgccgaagta
     5761 cgaataggca gaggtttccg gcaaatgact ttttcgatga gggcgccgct cgctgcatgg
     5821 cggcagatgc cgtcatcgcc ggggtttacc tgccccctgc tgcaccccac actgccggcg
     5881 atatcggtgc ggtgcaccat gacatgtccc ccgatgtctc cggattaatt gatccggatt
     5941 gttgtttttt tgtgagcgca ggtcacccgt cgagacctcg aaaccgaaat ccctcgtccg
     6001 aaaacggata ttctctacgc tcgctatcgt tgatcgcatc cagcctagga caaggtgttg
     6061 aactgtcaag cggcaagaaa tgccgccggg ctccaccgtt gcgcgcgccc gcggccgctg
     6121 cgcgattttc ggagacacgg ggtggcaacc tgcggcatga cacccccggt gatccggcgg
     6181 ctgcgcgcat ggcagacacc cgatccctga gctactcgtg accgaaagcg atttgccatg
     6241 aggcggcagg acccgtagtt tggcatcagt aaagcaacaa gaattgccgt tcggagagaa
     6301 aactcatggc gcgcctgaaa ttcggagcct tcctcgcccc gcatcatccg atcggcgagc
     6361 atccgatgct gcaattccgt cgcgacctgg atttggtcga gcagctcgac gcgctcggtt
     6421 acgacgagtt ctggtgcggc gagcatcatt cctcgggctg ggaaaccatc gcgtcgccag
     6481 aaatgttttt ggccgcggcc ggcgagcgca ccaaacgcat caagctcggc accggcgtgg
     6541 tgtcgctgcc ttatcaccac cccttcaacg tcgcccaacg catggtgcag ctcgaccaca
     6601 tgacgggtgg tcgcgccatc ttcggttccg gaccgggtgc gctggcctcc gatgcgcata
     6661 cgctcggcat cgatccgatg acgcagcgtg accgtcagga cgaggcgatc gcggtcattc
     6721 gccggctgtt caacggcgaa cgcgtcaccg ccaggagcga ctggttcacc atgaatgacg
     6781 cggcgctgca gatcctgccg ctgcaggagg agatgccgtt cgtggtggcg tcgcagattt
     6841 cgccgtcggg gatgacgctg gccggcaaat acggcatcgg catcatttcg ctcggctcga
     6901 tgtcgacgca aggcctgatg gcgctgccga cacaatgggg ttttgccgag gatgccgcca
     6961 ggaagcacgg cactacggtg agccggtcca actggcgcgt attgttgagc tggcacatcg
     7021 ccgagacccg cgagcaggcc cggcgcgaag ccggcgcagg actgatgcgc tggcataatg
     7081 aatataatgt cggcacactg cagcggcccg gcctgcagcc gttcacttcg cccgaggatg
     7141 ccgtggagaa aaccgccggc ggcgaagccg cggcttccac catcggcacg ccggacgatc
     7201 tggtgaaaac catcaagagc ctgatggatg tctctggcgg cgtcggcacc atcgtcggct
     7261 tcgtgcacga ctgggccaac ccggaaaata cccgccgcag ctgggacatg gtggcgcgct
     7321 atgtggtgcc ggaaatcaac ggttatatcg acggcttgcg caaatcgcag aaattcgtga
     7381 tcgagaatcg cgcggtgttc gagcgcgccg gccaagccgt gatggcgaag atcatggaga
     7441 acgacaaggc cgcggcagcg cttcccctca ccggccccgg acgcgtcgcg atcccgacca
     7501 tcaacgcgcc ggatctgcag aaggaagcgg cgaaacagaa ggcgtagcga ggacagcgtg
     7561 gcgcgatcgg ctatccgcct ggtcccccaa ccatttggat gacacttgtc ggtcgcaccg
     7621 acattgagct cagactgctt gccgatggcc gcgcggcttt gtcgcgctat ttggatcggc
     7681 cgagaccccg gcccgccctg cttgaggctt tgcacaggac gggcaaaggg ttatttactg
     7741 gcatcggcct cgcttgtcgg ggaccaaccg gagcaattgc catgtcgatg caaagtgttg
     7801 cttcccccgc gatcagcttc acgccgccga agcgcaattc gctcacgcat attcccggcg
     7861 acgagggctg gccgctgatt ggcaagacgc ttgagatcct cgccgatccc aaggggcagg
     7921 tcgaacggca ggccgccaga tacgggctgg tctatcgcag ccacatcttc ggcgagacca
     7981 gcctgtcgct gctcggcccc gaggccaatg agctggtgct attcgaccag gccaagcaat
     8041 tctcctcgac gcatggctgg ggccggattc tcggcctgct gtttccgcgc ggcctgatgc
     8101 tgctcgattt cgaggagcat cgcctgcacc ggcgcgcatt gtcggtcgcc ttcaaatccg
     8161 gaccgatgaa atcctatctc gccgcactcg ataccggcat cgcggcgcgg gtcgcacaat
     8221 ggaaagcgca gccggggccg atgctgttct atccggcgat gaaacagctc accctcgacc
     8281 tcgccgccac ctcgttcctc ggcgccgaga tcgggcccga ggtcgacgag atcacgcgcg
     8341 ctttcgtcga catggtggcg gcttccgtcg ccgtgattcg caaaccgttg ccgggcaccg
     8401 cgatggcgcg cggcgtccgg ggccgcaagc gcatcgtggc ctacttctcg gaacagattc
     8461 cgatccgccg cgccaaaggc ggcggcgaag atttgttctc gcagctgtgc cacgccaccc
     8521 atgaggacgg cgcgctgctg tcgacgcagg atattgtcga ccacatgagt ttcctgatga
     8581 tggccgcgca tgacacgctg acctcgtcgc tgtcgtcttt tgtgggtgcg ctcgccgccc
     8641 atccgcagtg gcaggacaag ttgcgtgacg aagtgacggc gctgggtgtg gccaccggcg
     8701 aggcgacgac attcgataat ctcgaggcca tgccgttgtc ggagatggcg ttcaaggagg
     8761 cgatgcggct gaaaccgccg gtgccctcga tcccgcgccg taccatccgg gatctcacct
     8821 tcaaaggcta cgcgatcccg gccggcacca tggtcggcgt caatccgctg ttctcccacc
     8881 acatgccgga catctggccc gatcccgaaa ggttcgatcc gatgcgcttt accgacgagg
     8941 cgcagcgcgg ccgccatcgc ttcgcctggg ttccgttcgg cggcggcgcg catatgtgcc
     9001 tcgggctgca cttcgcctat atgcaggcga aatgcttcac gcggcatttc ctgcaaaatc
     9061 tcaccgtctc gctggagccg ggctacacgc cggactggca gatgtggccg atcccgaagc
     9121 cgcgggacgg attgcgggtg acgctgtcgc cggttacgta agcggatcgg gcctcaagcc
     9181 aaggcaaggt ccggtaaagg ttgcgcgtcg atgccgacca cgtcgatgcc atcggcgcgg
     9241 ccacgaatcg gcaaacgccc gagatcgcga accgcaaacg gcggcgccga gctgaagcgc
     9301 tccatcgcgg cgcgggacgc cagaaagccg ccgtccacgt tccggctgag ttcttcaagc
     9361 cttgccgcgg tattcatgac gtcgccgttg aaaacgatcg cgcgtttgac gtcaccgatc
     9421 tcgccgacga tcaccggccc gaaatgcagg ctgccgcgaa tacgcggcgc cgctccgaat
     9481 tcgcgctcga gttggcccga tgcgcgcgac aattcatcgc gcatcgccac aaagcatcgc
     9541 agcgggcgac aatcgatcgc gccgccgcgt tccggccagg tcacgatcac ctcgtcgccg
     9601 acataattga gcacttcgcc gcgatagtcc acgaccgcaa cggtgagcag acgaaacgtg
     9661 cggtcgagaa gacggtgaat ggcgatcccg ccgagccgct cggccagtcc ggttgatccg
     9721 gcaatgtcga cgaacagcac gaagcgtttc tcctcgaccg gcgtatggta ccgcccgatg
     9781 aagaaattca ggaatgcgcg gggtccgatg atgttggtta ccccgagagc cagattcatg
     9841 acgaccgaga gcaccgcgga gtagataatg ctggaccaga aagttgacgt gtcgggggac
     9901 agtccggcga tgacctcacc caactgaaac cactggatga cagtgataat cgcgacatag
     9961 atcgtgctcc gcaccatcag acgggttgcg aacgaaagac caccgagcca ctcgcgtatc
    10021 ggtccttcta aaacgaacag ctcggtgctc ccgagcgtga cggacatcag caggccatac
    10081 gaggctccca ctgccaaaaa ggtgggcgag gtgcggccct gcgcgacatc aaacaccatt
    10141 cccgcgatga gcccggccgc gacgagggca gcaagagatc gcagttttcg ttcaagcctt
    10201 gcgttcatgc cgcctaccca cgcgttgccg acgccgctga ttatacacgc gcgtgtcggc
    10261 ggcccgacgc ctggagcgcg gcgagggatc gctactcgac aaaggtcgtg agctgggcgc
    10321 cctcgtccgc tacgaacacc gcgatcagtt cggctgtttc ggtggcgctc gcattcgccg
    10381 agatcagatg cgttgcgccg ggcggctcga agaaggattg cccgaccttg aaggtctcaa
    10441 cggggccgcc ggcaagctgc gagcggattt cacctttggt gatgtaggcg gtgacggagc
    10501 ccgcgtggcg atgcgcaggg gtaaacccgc cgggcccgta gaatacgcgc acgatcgtca
    10561 cgcgcttccc cggcacattg ggcagcgcgt gcgagctgat cggttcgacg gtatcgggcg
    10621 tcgatcccga ggtgtcgccg accgcgcaca gcggcgcaag caccgtcgaa agcgtatcca
    10681 tcatgatggg cagcgttttc ccgatcacga aagcgcacgc cagcccggcg ataattgctg
    10741 cataaagtgg tcgggcttca ggtcgcgtct gcatcatagc ggctgttgtg gacatgatga
    10801 tctccgcagt ctggttcaaa ttgtatacaa cccggattcg tcattccggg agggtgctgg
    10861 caccagaccc ggaatgacgg cggcccttat ccggcgccct gatcgaacgc cttgcgcaag
    10921 gcaacatagc cctgctgttg ctgggtccag ttgcggccgc cggtgatggc gccatcgacc
    10981 accagatcat ggccgttgat gaaactggat tcgtcgctgg cgaggaacac cgcggcgtgg
    11041 gcgatgtctt cgggcaaacc ggcgcgcggt atgggctgcg cggtcttgta gacttcgcgc
    11101 atcacatcgg acgtcttctc cgccgcctcg accgaaagtc cgagcgcctt gccgaagata
    11161 ccggtggcga tcgcgcccgg cgaaatgctg ttgacgcgga tgccggattc gccaagttcc
    11221 atcgccacgc atttggtgag gtgaatgacc gcggccttgg ccgccccata gaccatcgac
    11281 gacgagaaac cggcgagcct tcccgcgatg ctgccattgt tgatgatgct gcccgacccc
    11341 tggcggcgca tatga
//
LOCUS       DASWGM010000670         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1873765,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000670 DASWGM010000000
VERSION     DASWGM010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873765"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VGA15_18855"
     CDS             complement(<1..909)
                     /locus_tag="VGA15_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9589791.1"
                     /translation="MSNSKDAVRKILDLVKADKRTSLTAPEGKLVCDAYGIPVPKEGV
                     AKSASEAAKIAADMGFPVVMKIVSPDILHKTEAGGVMVGVKTAADVEKNYATILANAK
                     KYKSDAKIEGIQVQQMLLGGQEVIVGAVTDGSFGKLVAFGLGGVLVEVLKDITFRLAP
                     ATKQDALSMLDGIQAHEMLKGVRGSDPANRDAIADIIVNVSTLTTDFPEITEMDLNPV
                     FATRDGAIAADVRIVVDFEPKPPRPRPKHDDIVRQMNRIMKPKAVAVIGASAENGKIG
                     NSVMKNLINGGYKGEIYPIHPKADEIM"
     gene            1236..1967
                     /locus_tag="VGA15_18860"
     CDS             1236..1967
                     /locus_tag="VGA15_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9589792.1"
                     /translation="MPIRKQTKAPPNMAEAEIAIVRIAPETSFKNKAYDALKEAILKM
                     DIYATPEQVMLDERALSERLGVSRTPIREAIAMLEQDGFVKTVPRRGIVVVRRTKSEI
                     VDMIRAWAALESMAARLITTTARKKDISALRDFFKNFNKDRLPQDHVEEYSKANIAFH
                     QALISLSESPVLVDMTNDILLHVRGYRQLTIGRTDRTATSLPEHMAIIEALEERDTEL
                     AEKRARDHTLGLAAYVEAHGQELFS"
BASE COUNT          382 a          598 c          595 g          419 t
ORIGIN      
        1 catgatctca tcggccttcg gatggatcgg gtagatctcg cccttgtagc cgccattgat
       61 gagatttttc atcacggagt tgccgatctt gccgttctcg gcggacgccc cgatcaccgc
      121 aaccgccttc ggcttcatga tccggttcat ctggcgcacg atgtcgtcat gtttcggacg
      181 cgggcgcggc ggtttcggtt cgaagtcgac gacgatgcgc acgtcggcgg cgatcgcgcc
      241 atccctggtc gcgaacaccg ggttgagatc catctcggtg atttcaggaa aatccgtggt
      301 cagcgtcgag acgttgacga tgatgtcggc gatggcgtcg cggttggccg gatcgctgcc
      361 gcgcacgccc ttgagcatct catgggcctg gatgccgtcc agcatcgaca gcgcgtcctg
      421 cttggtcgcc ggcgcgagcc ggaaggtgat gtctttgaga acctcgacca gcacgccgcc
      481 gaggccgaag gccacgagct tgccgaacga cccgtcggtg acggcgccga cgatgacttc
      541 ctgcccgccg agcagcatct gctggacctg gatgccttcg atcttggcgt cggacttgta
      601 tttcttggcg ttcgccagaa tggtcgcgta attcttttcg acgtcggccg cggtcttcac
      661 cccgaccatc acgccgccgg cttcggtctt gtgcagaatg tccggcgaga cgatcttcat
      721 cacgaccgga aagcccatat cggccgcgat cttggcggcc tcgcttgcgg atttggccac
      781 gccctctttt ggaacgggaa tgccgtaagc atcgcagacc agtttgccct ccggcgccgt
      841 caggcttgtg cgtttgtccg ctttgacgag atcgagtatt ttgcggaccg catctttgga
      901 attcgacatt ggcatcctcc ttgaggtcac ttttttagtt cgtctttaaa ctcgtcggca
      961 gcgcgcgcgg acatcaggac cgtgataccc ccggcacgcc ttcaatcgag gttcccgatg
     1021 atcgcaaacg cggttccgcg ttagcttgcg gcattggttc tgcggcccct ggcggcgcgg
     1081 gatggcattt ggtatgccag caatgacctt gtcaaggcaa gggtgtcaac gcaagtgctg
     1141 aaaacacggg caacttgaca tgctggtatt tggtatgcca aaaatcgaac cgaatttctt
     1201 ctgctagaag aggtctccct cggaggagat caatcgtgcc catacggaaa caaaccaagg
     1261 cgccgcctaa catggctgag gcagaaattg caatcgtccg catcgcgccg gagaccagct
     1321 tcaagaacaa ggcctacgac gccttgaagg aagctatcct caagatggac atttatgcga
     1381 cgcccgagca ggtcatgctt gacgagcgcg cgctgtcgga gcgtctcggc gtcagccgga
     1441 ccccgatccg cgaagcgatc gcgatgctgg agcaggacgg gttcgtcaag accgtgccgc
     1501 gccgtggcat cgtcgtcgtg cgccgcacca agagcgagat cgtcgacatg atccgggcct
     1561 gggccgcgct ggagagcatg gcggcacggc tgatcacgac gacggcgcgc aaaaaggaca
     1621 tttccgcgtt gcgcgacttc ttcaagaatt tcaacaagga tcgcctgccg caggatcacg
     1681 tcgaggaata ttccaaggcc aacatcgcct ttcaccaggc gctgatctcg ctttccgagt
     1741 cgccggtact ggtcgatatg accaatgaca tcctgctgca cgttcgcggc tatcggcagt
     1801 tgacgattgg gcggacggac cgcaccgcaa cctcgttgcc ggagcatatg gcgatcatcg
     1861 aagcgctcga agagcgtgac accgagcttg cggaaaagcg ggcacgcgat cacacgctcg
     1921 gccttgccgc ttatgtcgaa gcgcacggcc aagagctatt tagctgacca cagcctttta
     1981 ttctggacga cgac
//
LOCUS       DASWGM010000671         5949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2023260,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000671 DASWGM010000000
VERSION     DASWGM010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023260"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VGA15_18865"
     CDS             <1..293
                     /locus_tag="VGA15_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX9589793.1"
                     /translation="ASGTSRMDNKKLKARFGGKPKMLGVDEVADITGHEVGGVCPFGL
                     KTPLPVYCDVSLKVFDEVVPAAGSTHSAVRIAPSRMAELVAAEWVDVCQEPA"
     gene            566..784
                     /locus_tag="VGA15_18870"
     CDS             566..784
                     /locus_tag="VGA15_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589794.1"
                     /translation="MLNGDAAADRYHKAQDKQAGSHLPILLLEIHFPVHVPRGRAKHL
                     ASSLKINEAREGSERTTKCESGAFRKEK"
     gene            complement(828..1739)
                     /locus_tag="VGA15_18875"
     CDS             complement(828..1739)
                     /locus_tag="VGA15_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9589795.1"
                     /translation="MAKLPDFEGLAIFAKVVELRSFAGAATELALSKATVSKAVSRLE
                     QRLGARLFNRTSRRLALTDAGQKLSERAARLLADGEAAENEALAQSATPRGLVRLAVP
                     MTFGVSKVAPILPEFLGQYPDVAIDLHLSDAMVDLIGEGFDAGLRIASLPDSSLIARR
                     LCAMPRYTVASPAYLKRHGRPTHPMHLAQHKCLGYAYLSTPDVWHYTNAAGEQVSVRP
                     AGPLRVNNGEALMPALLAGLGIADLPDFIVGDAVASGEVEVILKGWKQAEGAVHLVTP
                     PGGPRPARVEVLADFLTKHFAKEKKRK"
     gene            1864..2562
                     /locus_tag="VGA15_18880"
     CDS             1864..2562
                     /locus_tag="VGA15_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9589796.1"
                     /translation="MIELRPFAKLGGADHGWLKAKHHFSFGSHYDPGNMGHGSLRVWN
                     DDEIAPNTGFPAHPHANMEIITYVREGAITHQDSLGNKGRTEAGDVQVMSAGSGVRHS
                     EYNLEPTRTKIFQIWIEPTTEGGQPTWGAKPFPKSDRSGNFVTIASGFDGDKDALPIR
                     ADARVLATTLKAGESAEYASGKTRNLCLVPAAGAVEINGVRVNARDGVAIRNEAKLKI
                     TALEDSELVLVDAA"
     gene            2734..3333
                     /gene="wrbA"
                     /locus_tag="VGA15_18885"
     CDS             2734..3333
                     /gene="wrbA"
                     /locus_tag="VGA15_18885"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HEX9589797.1"
                     /translation="MTRVLVLYYSAYGHIEKMASAVAEGAREAGAAVDIKRVPELVPP
                     EVAKASYYKLDQPAPVAKIEDLANYDAIIVGTGTRFGRMASQMANFLDQAGGLWMKGA
                     LHGKVGGAFTSTATQHGGQETTLFSIIANLLHFGMAVVGLNYGFAGQMKLDEVTGGSP
                     YGATTIAGGDGSRQPTENELAGARYQGRVIAETAKKLHG"
     gene            3403..3534
                     /locus_tag="VGA15_18890"
     CDS             3403..3534
                     /locus_tag="VGA15_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589798.1"
                     /translation="MIEMLFIYQLARNPVQAVARRWWCRSLYRASRGKRRCPECTKS"
     gene            3639..3815
                     /locus_tag="VGA15_18895"
     CDS             3639..3815
                     /locus_tag="VGA15_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589799.1"
                     /translation="MTNKPDPKGTSKNPAEPHPGMMGDGTEEQNLGQPGNPGARIKKD
                     EVDAAFGKDAKKTK"
     gene            3919..4866
                     /locus_tag="VGA15_18900"
     CDS             3919..4866
                     /locus_tag="VGA15_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX9589800.1"
                     /translation="MSDAAQAPWPELPTAAWRETCETLHLWTQIIGKIRLTRAPWLNH
                     SWHVALYVTARGLTTSPIPDGTRTFQIDFDFIDHVLRVSTSDGVGRQLALAGQSVASF
                     YAAVMADLADLGIHIAIDETPNELPEPIRFSQDRKHASYDPTAVRRFLQILVNADRVF
                     KQFRTGFLGKASPVHFFWGSFDLAVTRFSGRRAPRHPGGVPHLSDAVACEAYSHEESS
                     AGFWPGSGAIDYPAFYSYAYPEPAGYRATRVRPDAAFFSEALGEFILPYDAVRTAAQP
                     DQALLEFLQSTYEAAADTAKWDREALECAPGQPGVVRQI"
     gene            complement(5025..5627)
                     /locus_tag="VGA15_18905"
     CDS             complement(5025..5627)
                     /locus_tag="VGA15_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEX9589801.1"
                     /translation="MKRLSTLAAGLLIGAAALQYSNLASGQSEGGWITLLDGTKMGDW
                     NEVGKANWAMKDGALTVDKLDGKELSYLVTKNSYKDFQIRAEFWTDEEANSGVFIRCD
                     QSQKIDAKICYEVNIYDKRPDPSYGTGAIVDVAKVDPMPKAAGKWNTYEITAQGPHLV
                     IVLNGQKTADVQDSKHLSGPIALQYGSGVVKFRKVQIKEL"
BASE COUNT         1176 a         1895 c         1803 g         1075 t
ORIGIN      
        1 tcgccagcgg cacctcgcgg atggacaaca agaagctcaa ggcgcgcttc ggcggcaagc
       61 cgaagatgct gggcgtcgac gaggtcgccg acatcaccgg ccatgaagtc ggcggcgtct
      121 gcccgttcgg attgaaaacg ccgctgccgg tctattgcga cgtgtcgctc aaagtgttcg
      181 acgaggtggt gccggccgcc ggctcgaccc acagcgcggt gcggatcgcg ccgtcgcgga
      241 tggcggaact ggtcgctgcc gaatgggtcg atgtctgtca ggagccggcg tagcgaccgg
      301 tctattggtc gtcatcgtaa ggcctcggcc gatcgtcgcg acgtctcggt cggtcgtcgc
      361 gccgtctcgg cgcagcctgc tggttcgcgc tgacattcgg ttcaaacgcc gcgcggcacg
      421 gcccagagag ctgcggggtg ttttgccgta ggcatgcaac gattcgatcg acgtcgggta
      481 tctgttcgcc gcaaagccgg aatacatcgg gcgtgcaggc catctgctgt tccatcgttc
      541 ctcgattctc ctgcgaaaac gcgggatgtt gaacggcgat gccgcagccg atcgatatca
      601 caaggctcag gacaaacagg cgggctcgca tttaccaatc cttcttttgg agattcattt
      661 tcccgtccat gtaccgcgcg ggcgcgccaa gcatcttgcc tcaagcttga aaataaatga
      721 ggcccgggaa ggttctgaac ggacaacaaa atgtgaaagt ggcgctttta ggaaagaaaa
      781 gtgagaaaaa cgctcaagtt gcgatagtcg cgcaagggcg aaacaaacta cttccgcttc
      841 ttctccttcg caaaatgctt ggtcagaaaa tccgccagca cctcgacgcg cgcgggccgc
      901 ggtccgcccg gcggcgtcac cagatgcacc gcgccctcgg cttgcttcca gcctttcagg
      961 atcacctcga cctcgcccga cgcgacggcg tcgccgacga tgaaatccgg cagatcggcg
     1021 atgccgagcc cggcaagcaa cgccggcatc agcgcctcgc cattgttgac ccgcagcggg
     1081 cccgcggggc gcacgctgac ctgttcgccc gcggcgttgg tgtagtgcca gacatccggc
     1141 gtggaaagat acgcatagcc gaggcatttg tgctgggcga gatgcatcgg atgggtcggc
     1201 cggccgtgcc gcttgagata cgccggcgac gccaccgtgt agcgcggcat cgcgcatagc
     1261 cggcgcgcga tcaacgagga atccggcagg cttgcgatcc gcaaaccggc atcgaagccc
     1321 tccccgatca gatccaccat ggcatcgctc aagtgcagat cgatcgcgac gtcgggatac
     1381 tggccgagaa attccggcag gatcggcgcc actttgctca ccccaaacgt catcggcacc
     1441 gcgaggcgca ccagcccacg cggcgtcgcc gattgcgcca gcgcctcgtt ctccgccgcc
     1501 tcgccatcgg cgagcaggcg cgccgcgcgc tcggacagct tttgcccggc atcggtcaag
     1561 gcaagccgcc gcgaggtgcg gttgaacagc cgcgcgccga gccgctgctc cagccggctg
     1621 acggccttcg agaccgtcgc cttggacagc gccagttcgg tcgcggcccc cgcaaacgac
     1681 cgtagctcca cgacttttgc gaagatcgcg aggccttcga aatcggggag ttttgccatt
     1741 tgtacatccc attagagcta ttttggaaac aattagtttc aatagtttct atttatgcac
     1801 catacggaag cgcatatcca gatgccaacg aaatttgaaa tccaacgatc aaaggaaaat
     1861 cccatgattg aactcaggcc ttttgcaaaa ctcggcggcg ccgatcacgg ctggctaaag
     1921 gcaaaacatc acttctcgtt cggcagccac tatgaccccg gcaatatggg ccacggctcc
     1981 ttgcgggtat ggaacgacga cgagatcgcg ccgaacaccg gctttcccgc ccatccccat
     2041 gccaacatgg aaatcatcac ctatgtccgc gaaggcgcca tcacccatca ggacagcctc
     2101 ggcaacaagg ggcgcaccga agccggcgac gtgcaggtga tgagcgccgg atccggcgtg
     2161 cgtcactccg aatacaatct ggagccgacc aggaccaaaa tcttccagat ctggatcgag
     2221 ccgaccaccg aaggcggcca gccgacctgg ggcgcaaaac cgtttccgaa gtcggatcgc
     2281 tccggcaact tcgtcaccat cgccagcggc ttcgatggcg acaaggatgc gctgccgatc
     2341 cgcgccgatg cgcgggtgct cgccaccacc ctgaaggccg gcgagagcgc ggaatatgcg
     2401 tccggcaaaa cccgcaacct gtgtctcgtg ccggcggcgg gtgcggtcga aatcaacggc
     2461 gtgcgcgtca atgcccgcga cggcgtcgcg atccgcaacg aagcgaagct gaaaatcacc
     2521 gcgctggaag attccgaact ggtgctggtc gacgcggcgt aaggccgaac ctctcgttcg
     2581 tcatgcccgg ggcaaaagcg cgaagcgcgt cttcgcgctg gatgacccgg gcatccatcc
     2641 ctcttcggaa gagtctttcg aaggtgatgg attgccgggt caagcccggc aatgacgagt
     2701 tctaaatcga tcccaacccc tcggagaccc accatgacaa gagttctcgt tctctattat
     2761 tccgcttacg gccacatcga gaagatggcg agcgccgtcg ccgaaggcgc ccgcgaagcc
     2821 ggcgccgccg tcgacatcaa gcgagtgccc gaactggtgc cgccggaggt cgcaaaggcg
     2881 tcgtactaca agctcgatca acccgcgcct gtggctaaaa tcgaagacct cgccaactac
     2941 gacgccatca tcgtcggcac cggcacccgc tttggccgca tggcatcgca gatggcgaat
     3001 ttcctcgatc aggccggcgg cctctggatg aagggcgcgc tgcacggcaa ggtcggcggc
     3061 gccttcacct cgaccgcgac ccagcacggc ggccaggaaa ccacgctgtt ctcgatcatc
     3121 gccaacctgc tgcatttcgg catggccgtc gtcggcctga actacggctt tgccggccag
     3181 atgaagctgg acgaggtcac cggcggctcg ccctatggag cgaccacgat cgcaggcggc
     3241 gacggcagcc gccagccaac cgaaaacgag ctggcgggcg cgcgctatca aggacgcgtg
     3301 atcgcggaga ccgcgaaaaa actgcatggc tgatgcgacg cgggcggcat tctcttcggc
     3361 ggatgccgcc ccatttaggt tgtggggacg atggaattcg ccatgatcga aatgctcttt
     3421 atctatcaac tggcacgcaa tccggttcag gcggtcgcgc gacgctggtg gtgtcgcagc
     3481 ctctaccgcg catcgcgcgg caagcgccgc tgcccggaat gcaccaagtc gtgaagccgg
     3541 accactcgtt caagtggtaa acttgggtca gtgaggcggt ggaaccagac cgcattcatg
     3601 cagttgggtt acgcgcaccc gcgcccggag atttcgaaat gaccaacaag ccagatccca
     3661 aaggtaccag caagaacccc gccgagccgc atcccggcat gatgggcgac ggcaccgaag
     3721 agcaaaatct cggccagccg ggaaatcccg gggcccgcat caaaaaagac gaagtcgacg
     3781 ccgccttcgg caaggacgcc aagaagacaa aataacgcgc atccccaatc gtggtaagtt
     3841 cgcatataaa ggccgggccg gatcgcgccc ggcctcgtcg tgtcaggaga tgccgatgac
     3901 aaccagttgc ggtgaaccat gagcgatgcc gcgcaggcgc cctggccgga actgcctacg
     3961 gcggcgtggc gggagacctg cgaaacgctg catctgtgga cccagatcat cggcaagatc
     4021 cgcctgacgc gcgcgccctg gctgaatcat tcctggcacg tcgcgctgta cgtcaccgcg
     4081 cgcggactga ccacctcgcc gatccccgat gggacgcgga ccttccagat cgatttcgac
     4141 ttcatcgatc atgtcttgcg ggtctccacc agcgacggcg tagggcggca attggcattg
     4201 gccggacaat cggtagcgag cttctacgcc gctgtcatgg ctgaccttgc agacctcggc
     4261 attcacatcg ctatcgacga gacgcccaac gaattgccgg aaccgatccg gttttcgcaa
     4321 gacaggaaac atgcatccta cgatccgacc gcggtgcggc gctttctgca gattctcgtg
     4381 aatgccgacc gggtcttcaa gcaattccgc accggcttcc tcggcaaggc aagcccggtg
     4441 catttcttct ggggcagctt cgatcttgca gtcacacgct tttccggccg gcgcgcgccg
     4501 cgccatcccg gcggcgtgcc gcatctatcc gatgcggtgg cctgcgaagc ctattcccac
     4561 gaggaaagca gcgccggctt ctggccgggc agcggcgcga tcgattatcc cgcgttctat
     4621 tcctatgcct accccgagcc ggcgggttat cgggccacgc gggtgcggcc ggatgcggcg
     4681 ttcttcagcg aggcgctcgg cgaattcatc ctgccctatg acgccgtgcg caccgccgcg
     4741 cagccggacc aagcgttgct cgaatttctg caaagcacct atgaggccgc ggccgatacc
     4801 gccaaatggg accgtgaggc gctggaatgc gcgccgggcc agccgggcgt ggtgcggcag
     4861 atttagttct cttcgcgcga aggcggcctg ccctgctcgc gtttcacatt ggggacgagc
     4921 cgcggatagc ggcgaaggag tcggcgcaag tcacgcaagc ttcgcgcacg atggtggacg
     4981 cacctcgtgc cccggatgca gcgcatccgg ggcacgaggc tgctctacag ctccttgatc
     5041 tgcaccttgc ggaacttgac cacgcccgaa ccgtattgca gcgcgatcgg gccgcttaag
     5101 tgcttgctgt cctgcacgtc ggcggtcttt tgaccgttca gcacgatcac gagatgcggg
     5161 ccctgcgcgg tgatttcata ggtattccat ttaccggcgg ccttcggcat cggatcgacc
     5221 ttggcgacat cgacgatcgc cccggttccg taagatggat ccggccgctt gtcatagata
     5281 ttgacctcgt agcagatctt ggcatcgatc ttctgcgact ggtcgcagcg gatgaacacg
     5341 ccgctgttgg cttcttcgtc ggtccagaac tcggccctga tctggaaatc cttgtaggaa
     5401 ttcttggtga cgagataaga cagctccttg ccgtcgagct tgtcgacggt gagcgcgccg
     5461 tctttcatcg cccaattggc tttgccgacc tcattccagt cgcccatctt ggtgccgtcg
     5521 agcagcgtga tccagccgcc ttcgctctgg ccggaggcca ggtttgaata ttgcaaagcg
     5581 gcggcgccaa tcagcagacc agccgcgagc gtcgataagc gcttcatgaa gatgtcctcc
     5641 cgtttgtttt aattgggcgc aacgttagca ccgccgtgca gggcgtaaac gcgtttttca
     5701 tgctgccgtt gcgttgtgta tggtctcgca tgcccgcccg cgtcttgcac cgcgggaatg
     5761 agaccggcta gcatgaaccc aatgagagat gagctggcgc cagcgattca gctcgcggaa
     5821 ttcaacggag gaaatcatga aggccacaaa ccggttttgg cgatacctgg cgatgccgat
     5881 atgctttgcc ggcatgctga gcatcagctc ggcggccgaa ctcaatccgg ccgcggtgat
     5941 ctacaagct
//
LOCUS       DASWGM010000672         9039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1753191,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000672 DASWGM010000000
VERSION     DASWGM010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1753191"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /gene="serA"
                     /locus_tag="VGA15_18910"
     CDS             <1..1552
                     /gene="serA"
                     /locus_tag="VGA15_18910"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX9589802.1"
                     /translation="PAAVQIFKDRGIDVDFQPNLGKDKDKLAEIIGNYDGLAIRSATK
                     ATAKILEKATRLKVIGRAGIGVDNVEIPAATAKGIIVMNTPFGNSITTAEHAITLMLA
                     LAREIPQADASTQAGKWEKNRFMGVEITAKTLGVIGCGNIGSIVADRALGLRMKVIAF
                     DPFLSPERAKDIGVEKVDLDDLFKRADFVTLHTPLTEKTRNIIDAAALAKMKKGVRII
                     NCARGGLVDEQALVDALNSKHVAGAAFDVFVEEPATSNVLFGHPNVICTPHLGAATTE
                     AQENVALQVAEQMSDYLLSGAISNAVNFPSITAEEAPKLKPFIELAEKLGSFAGQLTE
                     SGILKVQITYEGHVAEMKIKALTSAALSGLLRPMLGDVNVVSAPVVAKERGMVVDEVT
                     RAAQSDYESLITVTVTTERQERSVSGTVYHDGKPRLVDIKGIRVDAEFGKSMIYVTNE
                     DKPGFIGHFASLLGDAKINIATFHLGRNKPGGDAIALVEVDGAVPPALLAKVQALPQV
                     RQAKALVF"
     gene            1913..3571
                     /locus_tag="VGA15_18915"
     CDS             1913..3571
                     /locus_tag="VGA15_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HEX9589803.1"
                     /translation="MHIAANRLVTTSLVVFAALAAMQAKAADVEATSAVDAVTVYPDG
                     ASVTRAITADVPSGDNTLVIKDFPLTLDPSSLRVEGEAGARLTIGAIDTRRPRAAPPV
                     DLPEIDKRIETLKDERANLDGAIAAATARRKFAERFAEASPAGIGDKGEARPIADWRV
                     AFAAVAEEVATADSAIRDAERKQRDIDREVARLESNRAIKPPSKLEVRIDLAAAAATK
                     ATLRVTYAVRAARWTPLYDARLDTGAKDRKPALELVRRAEITQTTGEDWSNVALSVST
                     VRSARGGKAPELNSLIVQYPPAVQPAPASSVVGNLRQDSHSRAMAKAPEPAAEKAEEQ
                     QAVTEVGGFQVVFKIPGRVSLGASEGAKSLRVSTATIAPDLAVRSVPLIDPTAFLEAS
                     FVQSEDAPLLPGRVAIYRDGVFVGRGQMMAAGKDEKVRLGFGADDKVKVERSVVKRNE
                     GSAGLILTTSKTDERAFKTVIRNGHDFPIRIAIEDQLPVSENEDIQVEMLPSTTPPTA
                     TNLRDKRGVLEWAFEAKPGEARDIAFAWRVRWPKDKGVVMMPAG"
     gene            complement(3690..3893)
                     /locus_tag="VGA15_18920"
                     /pseudo
     CDS             complement(3690..3893)
                     /locus_tag="VGA15_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018482954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6130 family protein"
     gene            3968..4333
                     /locus_tag="VGA15_18925"
     CDS             3968..4333
                     /locus_tag="VGA15_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589804.1"
                     /translation="MRKLILISVLALASVTAQAGQQRGLVLASSDAPNASERIESVNP
                     EARKAYVKPEAPRPARETSAPVKPASSPAKQVFTPAKKQVFTPVKQASMLANRVHARG
                     YDNQEAEARRIAALYGVSW"
     gene            complement(4419..5462)
                     /locus_tag="VGA15_18930"
     CDS             complement(4419..5462)
                     /locus_tag="VGA15_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX9589805.1"
                     /translation="MSKFKVVTPKGASFTVAGGGYAYEKEALDPIGAEIVEAPANEAE
                     FIAAARTADAIYGKGMPISKTIIDALENCKVITLGSVGVDSVDVKAATARGIPVTNIP
                     DTFIEEVADHAMMLLLAGFRRLVEQDKMVRSGRWAEGRPALLKIPRLMGQTLGFISFG
                     RVARAVAKRAAPFGLRMMAYDPFIAETLISDHGVLPATLSEVLSQSDFVSMHAPARPE
                     VHHMLGESHFRQMKPNAIFINTGRGATVNEEVLIKALQEGWIAHAALDVLEKEPPSHN
                     NPMLSMENVTMTAHVASASARFDEARKRRVGFELSLVLSGLWPASCVNPSVLQNTSLR
                     RWQPVSMERGPNS"
     gene            5699..7384
                     /locus_tag="VGA15_18935"
     CDS             5699..7384
                     /locus_tag="VGA15_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9589806.1"
                     /translation="MKLTNLTITPKLGILVGVTLLGLCAAGALAGYLMQREMLNARID
                     QTRAIVDMARNMAAGLQKQVEAGQLTKEAALAEFGRISNSMTYDNGAGYLFGTTYDGI
                     TVMSPDPKQIGTNRMEVVTNGRKLSHELMDGVLAKGEILLPYEYVKPGEEKPTRKIGY
                     AVAIPGFNMYLGTGAYLDDLDAKLKPIAWVLGLAILGIAVISGSIAWMISRSISRPLG
                     QLGARMRALADGALEGDIPGIARGDEIGAMAATVQIFKDNALRIRGLEKVEAETQARA
                     AAERRSAMESLASDFERSVNGIVRSVSTAAAGMQTTAQSMTATASDASARAATVGAAS
                     QSASNNVGTVAAAAEELSSSVTEISRQVQRSSEIASKAVGDAERTNATVGALSTGAEK
                     IGEVVKLIHSIAAQTNLLALNATIEAARAGESGRGFAVVASEVKALANQTAKATEEIS
                     AQVAAMQASTSDAVASIGGITETIAQMSEITVSISTAIEQQGNATREIARNIQSVAAG
                     SNEISAHIGGVTTAAAATGTAASDVLSNARELDNQSGMLRGAVDGFLAKVRAA"
     gene            complement(7546..8481)
                     /locus_tag="VGA15_18940"
     CDS             complement(7546..8481)
                     /locus_tag="VGA15_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9589807.1"
                     /translation="MTIPAAPPPAPDPLRWLNNQPYLLLSLTSLFWAGNIVLARYVAG
                     HVPPMTLSCVRWIGAFFMLLPFALPHLKRDWPALRVRLPLLVLLSATGFAINNALSYW
                     ALQYTQALNALLIQSSGPLFVALWSLTLFGVRLTWAQLSGIAISLLGVLTIILHGDIG
                     ALASIQFSKGDVMFAGALLAFGLYSALMPRRPKTHQLSLISFTTGCGALLLLPFSIWE
                     FSTGFTLKFDVLTVATLTYVVIFPSTLAYLFFNRGIALIGPNRAAPFFHLVPVFGSAM
                     AILLLGEQPRLFHLVGYLLVLAGVVIASRQASAGV"
     gene            8584..8985
                     /locus_tag="VGA15_18945"
     CDS             8584..8985
                     /locus_tag="VGA15_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX9589808.1"
                     /translation="MAVDLLNIRGLKELEHHGPVVMVNLMRFHDRSLDGNGSGWDAYL
                     RYSALTVPMIKARGGTLLWTGDAKAVALGKQEGNQWDYLALVYYPSVADFIDMMTSAD
                     YENLSDPHRRNGCAEHVIIATAEAYSKFKIG"
BASE COUNT         1769 a         2830 c         2919 g         1521 t
ORIGIN      
        1 tcccgccgcc gtgcagattt tcaaggatcg cggcatcgat gtcgatttcc agcccaatct
       61 cggcaaggac aaggacaagc tcgccgaaat catcggcaat tacgacgggc ttgcgatccg
      121 ctccgccacc aaggccaccg ccaagatcct ggagaaggca acgcggctga aggtgatcgg
      181 ccgcgccggc atcggcgtcg acaatgtgga gatccccgcc gccactgcga agggcatcat
      241 cgtgatgaac acgccgttcg gcaattcgat caccacggcc gaacacgcga ttaccttgat
      301 gctggcgctg gcgcgggaaa tcccgcaggc cgacgcttcg acgcaggccg gcaagtggga
      361 aaagaaccgc ttcatgggcg tcgagatcac ggcgaagacg ctgggcgtga tcggctgcgg
      421 caatatcggc tcgatcgtcg ccgaccgcgc gctgggcttg cgcatgaaag tgatcgcgtt
      481 cgatccgttc ctgtcgccgg aacgcgccaa ggacatcggc gtcgagaagg tcgatctcga
      541 cgatctgttc aagcgcgccg attttgtcac cttgcatacg ccgctgaccg aaaagacccg
      601 gaacatcatc gacgccgctg cactggcaaa aatgaagaag ggcgtgcgca tcatcaattg
      661 cgcccgcggt ggcctggtcg acgaacaggc gctggtcgat gccttgaatt ccaagcatgt
      721 cgcaggcgcc gcgttcgacg tgttcgtcga ggagccggcc acgtcaaacg tgctgttcgg
      781 ccatcccaac gtgatctgca cgccgcatct gggtgcggcc accaccgagg cgcaggagaa
      841 tgtcgcgctt caggtcgccg agcagatgtc ggactatctg ttgagcggcg cgatttccaa
      901 cgcggtcaac ttcccgtcga tcacggcgga agaggcgccg aagctgaagc cgttcatcga
      961 attggccgaa aagctcggct cgttcgccgg ccagctcacc gagagcggca ttctgaaggt
     1021 gcagatcacc tatgagggcc acgtcgccga aatgaagatc aaggcgctga cctcggcggc
     1081 gctgtcgggc ctgctgcggc cgatgctggg cgacgtcaac gtggtctcgg cgccggtggt
     1141 tgccaaggag cgcggcatgg tggtcgacga ggtgacacgc gctgcgcaga gcgactatga
     1201 gagcctgatc acggtgacgg tgacgacgga gcggcaggag cgttcggtct ccggcacggt
     1261 ctatcacgac ggcaagccgc ggctggtcga tatcaagggc atccgggtcg acgcagagtt
     1321 cggcaagtcg atgatctacg tcaccaatga ggacaagccc ggtttcatcg gccacttcgc
     1381 gagcctgctc ggcgacgcca agatcaacat cgcgaccttc catctcggcc gcaacaagcc
     1441 gggcggcgac gccatcgcgc tggtcgaagt cgacggcgcg gtgccgccgg cactgctcgc
     1501 caaggtgcag gcgctgccgc aggtgaggca ggccaaggcg ctggtgtttt gatttcttcc
     1561 cctctcccct tgtgggagag ggtggacgcg atgcgaagca tcgcggacgg gtgaggggtt
     1621 acgacccatc gatagaccga aacccctcat ccggcacgga cttcgcatag ccgatgcaaa
     1681 gcatcggcgt cctactaaag aacggcggcc gaaggccgcc tacggccacc ttctcccaca
     1741 aggggagaag ggaaaaaggc cgccgccgcc ccgcctcacc gaaacctcaa tcgaaccaat
     1801 tctcttgtca caacgtctca ccactggggg cgataccatt tccatgcgcc gggccctgcc
     1861 ggaccagttc cggtcttgcc gacggcgcgc ggaaacttgc gggagagaaa tcatgcatat
     1921 tgctgcgaac cgccttgtca ccaccagcct cgtggttttt gcggcgctgg ccgcgatgca
     1981 ggccaaggcg gccgacgtgg aggcaacatc ggcggtcgac gccgtcaccg tctatcccga
     2041 tggcgccagc gtcacgcgcg ccatcacggc cgacgttccc agcggcgaca acaccctggt
     2101 gatcaaggat ttcccgctga cgctcgatcc atcctcgctg cgggtggagg gcgaggcggg
     2161 cgccaggctg accatcggtg cgatcgatac aaggcggccc cgtgcggcgc cgccggtcga
     2221 tctgcctgaa atcgacaagc gcatcgagac gctgaaggac gagcgcgcca acctggacgg
     2281 tgcgattgcc gcggccaccg cgcgtcgcaa attcgccgag cgttttgccg aagcgtcgcc
     2341 tgcgggcatt ggcgacaagg gcgaggcacg tccgatcgcc gattggcgcg tggcctttgc
     2401 cgcggtcgcg gaggaagtag cgaccgcgga ctctgccatt cgcgacgcgg aacgcaagca
     2461 gcgcgacatc gaccgggagg ttgcccggct ggaatccaat cgcgcgatca agccgccaag
     2521 caagctcgag gtgaggatcg atcttgcggc tgccgcggcg accaaggcga cgctgcgggt
     2581 gacctatgcg gtgcgcgccg cgcgctggac gccgctctat gacgcccgtc tcgacaccgg
     2641 tgcgaaggac cgcaagcccg cgctcgaact ggtgcgccgc gccgaaatca cccaaaccac
     2701 gggagaggac tggtcgaatg tcgcgctcag tgtttcgacc gtgcgaagcg cgcgcggcgg
     2761 caaggcgccc gagctgaatt cgctgatcgt gcaatatccg cctgccgtgc agccagcgcc
     2821 cgcgagctct gtcgtgggta atctgaggca agattcccac tcacgagcca tggccaaggc
     2881 gccggagcca gcggcggaaa aggccgagga gcaacaggcc gtgacggaag tcggtggctt
     2941 ccaggtggta ttcaagattc cgggccgcgt cagccttggc gccagcgaag gcgccaagag
     3001 cctgcgtgtc tccaccgcga ccatcgcgcc cgatcttgcg gtgcgttcgg tgcccctgat
     3061 tgatccgacc gcgtttctcg aagcgagttt tgtacaaagc gaggacgcac cgttgttgcc
     3121 ggggcgggtc gcgatctatc gcgacggggt gtttgtcggt cgcggtcaaa tgatggccgc
     3181 gggcaaggac gaaaaagtac ggctcggttt cggcgccgat gacaaggtca aggtcgaacg
     3241 ctccgttgtc aaacgcaacg aaggatcggc gggcctgatc ctgacgacat cgaagaccga
     3301 cgaacgcgcg ttcaagaccg tgatccgcaa tggccatgat ttcccgatcc ggatcgccat
     3361 cgaggatcaa ttgccggtca gcgaaaatga ggacatccag gtcgaaatgt tgccatcgac
     3421 gacgccgccg accgcgacca atttgcgcga caagcgcggc gtgctggaat gggcattcga
     3481 ggccaagcca ggcgaggcca gggacattgc cttcgcatgg cgggtacgct ggcccaagga
     3541 caagggcgtc gtgatgatgc cggccggata acgcgataga ggcgagcggc gggcggcgaa
     3601 tttctgaatc cgccgctcgc ctcggctgtt gttgcgcggt ggggggcgcc ggtgctggca
     3661 ctcttcatct cgaccactcc ctgacatctt cacttattgc tgttcggctc cggggtttta
     3721 tgttcgggca gcgtgaattc caccatctgg ctggtgatga ccttgtgcgt ggggccggcg
     3781 agttcgcaca gcgccttgtg cgggccgggt ttcagaccga ccacgatcac cgtttcgccg
     3841 ctggcgtcga cgaagtgcca cggcgcgtcg tcgacggtga tgtggacgtg gccgcctgcg
     3901 tgattgtgct cacataacgg attgagccat tcgtttagct tcccgtcatc aagcacggga
     3961 gattcacatg cgtaaactta tcctgatatc cgttctggct ctggcatccg tgacagctca
     4021 ggctggccag caaagaggtt tggtcctcgc ttccagtgac gcgccgaacg cctccgagag
     4081 aattgaatcg gtcaaccccg aagcgcggaa ggcctacgtc aagccggagg cgccgcgacc
     4141 tgcaagggag acttcggcgc ccgtcaaacc ggcttccagc cctgcgaagc aggttttcac
     4201 gcccgcgaag aagcaagttt tcacgcctgt gaaacaggct tccatgcttg cgaacagggt
     4261 tcatgcaagg ggatacgaca atcaagaggc ggaagcgcgc cgcatcgccg cgctctacgg
     4321 cgtctcctgg tgatgcgcaa atatctggtc tggccgtgag gccctggctc gctctttcct
     4381 gacacgattc gatcagattg aatcggatcg tctgtttctt agctgttcgg gccgcgttcc
     4441 atgctgacgg gttgccagcg gcggagcgag gtattttgca gcacggacgg attgacgcag
     4501 cttgccggcc acaggccgga gagcaccagg gacaactcga acccgacgcg ccgcttccgc
     4561 gcctcgtcga aacgcgccga ggccgaggcg acgtgggccg tcatcgtgac gttttccatg
     4621 ctcagcatcg gattgttgtg cgatggaggc tccttttcca gcacgtcgag cgcggcgtgt
     4681 gcaatccagc cttcctgcaa ggccttgatc agcacctctt cattcacggt ggctccgcgc
     4741 ccggtattga tgaagatcgc gttcggcttc atctggcgga aatgggattc gccaagcatg
     4801 tggtgaacct cggggcgtgc cggcgcgtgc atcgacacga aatcggactg cgagagcacc
     4861 tccgacaagg tcgccggcag cacgccgtga tcggatatca acgtctccgc gatgaaggga
     4921 tcgtaggcca tcatccgcag gccgaagggc gcggcgcgct tggcgacggc acgcgccacc
     4981 cgcccgaacg atatgaagcc caatgtctgc cccatcaatc tggggatctt gagcagggcc
     5041 gggcggccct cggcccagcg gccgctccgc accatcttgt cctgctcgac cagccggcga
     5101 aagccggcca gcaacagcat catggcgtga tcggccactt cctcgatgaa cgtgtcgggg
     5161 atgttggtga cgggaatgcc gcgggcagta gcggccttga cgtcgacgct gtcgacgccg
     5221 acgctgccga gcgtgatgac cttgcagttt tcgagggcat cgatgatggt cttcgagatc
     5281 ggcatgccct tgccgtagat ggcgtcggcg gtccgggcgg cggcgataaa ctccgcctcg
     5341 ttggccggcg cctcgacgat ctcggcgccg atcggatcga gcgcttcctt ttcatacgca
     5401 tagccgccac cggcgaccgt gaaactcgcg cctttcggcg tcaccacctt gaatttcgac
     5461 atttcctgct cccgatttga catttcggtg ttggttatgg ccgcgacgcg acgccgctgc
     5521 ctcccgattc cagcctaccg accgactttt cgcatattcc cggctgcttg gggaggatgg
     5581 ctgcccgtcc gtccagcaat tttacggcgc tcataacgaa acgctaaagc ttgcactgta
     5641 ccttgtggcg acaagttccg ccaatcgcac ccctttcgcc attccccgga gaggccccgt
     5701 gaagctgacc aatctgacga tcacccccaa actcggtatt ttggtggggg tgaccttgct
     5761 cggcctctgc gccgccggtg ctctcgcagg ctatctgatg cagcgcgaga tgctgaacgc
     5821 gcgaattgat cagacccgcg ccatcgtcga catggcgcgc aatatggcgg ccgggctgca
     5881 aaagcaggtc gaggccgggc aactgaccaa ggaagccgcg ctggccgaat tcggccgcat
     5941 ttccaattcg atgacctatg acaatggcgc cggctacctg ttcggcacga catatgacgg
     6001 catcaccgtg atgtcgccgg atcccaagca gatcgggacc aatcgtatgg aagtggtgac
     6061 caatggcagg aagctctccc acgaattgat ggacggtgtg ctggccaagg gcgaaatcct
     6121 gctgccgtac gaatacgtga agcccggcga ggaaaagccg acccgcaaga tcggctatgc
     6181 cgtcgccatt cccggcttta atatgtatct cgggaccggg gcctatctcg acgatctcga
     6241 tgccaagctg aagccgatcg cgtgggtgct gggcctcgct attctcggca tcgccgtgat
     6301 ctcgggcagc attgcctgga tgatcagccg tagcatcagt cggccgctcg gccagctcgg
     6361 tgcccggatg cgggcgctgg ccgatggcgc gctggaaggg gatatccccg gtatcgcgcg
     6421 cggcgacgag atcggcgcga tggcggcgac ggtccagatc ttcaaggaca atgcactacg
     6481 gattcgcggg ctcgaaaagg tcgaggcgga aacgcaggcg cgggccgcgg ccgagcgtcg
     6541 ctcggccatg gagagccttg ccagcgactt cgaacgcagc gtcaacggca tcgtccgttc
     6601 ggtgtcgacc gcggcggcgg gaatgcagac caccgcgcaa tccatgaccg ccactgcgag
     6661 cgacgccagc gcgcgcgccg ccaccgtcgg ggccgcctcg cagagcgcct cgaacaacgt
     6721 cggaacggtc gcggcggctg cggaagagct gtcaagctcg gtcaccgaga tttcccgcca
     6781 ggtgcagaga tcgagcgaga tcgccagcaa ggcggtaggg gatgccgagc gcaccaacgc
     6841 caccgtcggg gcattgtcga ccggtgccga gaagatcggt gaagttgtaa aactgatcca
     6901 cagcatcgcc gcgcagacca atctgcttgc gctgaacgcc actatcgagg cggcgcgtgc
     6961 cggcgaatcc ggccgcggct tcgctgtcgt ggcctccgag gtgaaagcgc tcgccaacca
     7021 gaccgccaag gccaccgagg aaatctccgc gcaggtcgcc gccatgcagg cctccaccag
     7081 cgacgcggtt gcttcgatcg gcggcatcac cgaaaccatc gcgcagatga gcgagatcac
     7141 cgtcagcatt tccacggcga tcgaacagca gggcaacgcg acccgcgaga tcgcgcgcaa
     7201 catccagtcg gtcgcggcgg gatcgaacga gatcagcgcc catatcggcg gcgtcaccac
     7261 ggcggcggcg gccaccggca cggcggcctc ggacgtgctg tcgaacgcgc gcgaactcga
     7321 caatcaatcc ggaatgctgc gtggcgcggt tgatggattt ctcgccaagg tccgcgccgc
     7381 gtaaagctga tccggtcccc tcgggcgtgt ctcggacgcg gtgcagcgtt ctttacgctg
     7441 ctccgcagag ccgggaccca tagagataat gcgcaccgtg tggaccccgg atcagcagcg
     7501 caccgcttgc gcgctgcgca gcatccgggg cacgcatccg caaatctaca cccccgccga
     7561 cgcctgccgt gacgcgatca ccacgccggc cagcaccagc agatatccga ccaggtgaaa
     7621 cagccgcggc tgctcgccga gcagcaggat tgccatcgcc gagccgaaca ccggcaccag
     7681 gtgaaagaac ggcgcggcgc ggttcggccc gatcagcgcg atgccgcggt tgaagaacag
     7741 ataggccagc gtcgagggaa agatcacgac ataggtcagc gtcgcgaccg tcaacacgtc
     7801 gaatttcagg gtgaagccgg tcgagaattc ccagatcgag aacggcaaca gcaggagcgc
     7861 gccgcagccc gtggtgaacg agatcagcga cagctggtgc gtcttcggcc ggcgcggcat
     7921 cagcgccgaa tagagtccga acgccagcaa ggcgcctgcg aacatcacat cgcccttgct
     7981 gaactggatg ctggcgagcg cgccgatatc gccgtgcagg atgatggtga ggacgcccaa
     8041 aagcgagatc gcgatgcccg aaagctgcgc ccatgtcagc cgcacgccga acaatgtcag
     8101 cgaccacaac gccacgaaca gcggtcctga cgactggatc agcagcgcgt tcagtgcctg
     8161 ggtgtattgc agcgcccagt aggacagcgc gttgttgatg gcaaaaccgg tcgccgacag
     8221 cagcaccagc agcggcaggc gcacgcgcaa ggccggccag tcgcgcttaa ggtgcggaag
     8281 agcgaacgga agcagcatga aaaacgcgcc gatccagcgg acgcaggaca gcgtcatcgg
     8341 cggcacgtgg ccggcgacgt aacgcgcgag cacgatatta cctgcccaga acagcgaggt
     8401 caggctcaac agcagatagg gctggttatt gagccatctg agcggatcgg gggcgggcgg
     8461 cggggctgcg ggaatcgtca ttgcttgacg gcagttgtgc cggaatcatg ccgcgcgaca
     8521 agtcccgccc ccgcaatgct acaatgcccc gacctcgttg aacgcaagcc taaggagccc
     8581 gccatggccg tggatttact gaatatcagg ggtctgaagg agctcgagca ccacgggcca
     8641 gtcgtgatgg tgaacctgat gcggttccat gatcgctcgc tcgacggcaa cggctcgggc
     8701 tgggacgcct atctgcgcta cagcgccctg accgtgccga tgatcaaggc gcgcggcgga
     8761 acgttgctgt ggaccggcga cgccaaggcg gtcgcgttgg gtaaacagga gggcaaccaa
     8821 tgggactatc tggcgctggt gtattatcca tccgtggctg atttcatcga catgatgaca
     8881 tcggcggatt atgaaaatct gtccgatccg catcgccgca acggctgcgc cgagcacgtg
     8941 atcatcgcga ccgccgaggc ctacagcaaa ttcaagatcg gctagaacgg cgccggtcgg
     9001 gcaacagcgg ttcaagcgcg ttgcggcagg ccgttttca
//
LOCUS       DASWGM010000673         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2023472,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000673 DASWGM010000000
VERSION     DASWGM010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023472"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /gene="metG"
                     /locus_tag="VGA15_18950"
     CDS             complement(<1..1539)
                     /gene="metG"
                     /locus_tag="VGA15_18950"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX9589809.1"
                     /translation="MPSQAERKSFYITTAIAYPNGVPHIGHAYEAIATDALARFQRLD
                     GKDVFFLTGTDEHGLKMIQTAELEKLPTLEVATRNALRFKEMDQRLNVSFDRFIRTTE
                     EQHHRSSREIWERMADNGDIYLDSYAGWYSVRDEAYYAEDETVVGEDNVRRGPQGTPV
                     EWVEEKTYFFKLSAYQDKLLALYADQPDFIGPDSRRNEVVSFVKSGLRDLSISRTTFD
                     WGVKVPGNSEHVMYVWVDALTNYITGVGFPDESNANWRYWPADVHIIGKDIIRFHAVY
                     WPAFLMSAGIPLQKRVYAHGFLFNRGEKMSKSVGNVVDPFNLANQYGVDQMRYFFLRE
                     VPFGQDGSYNHEAIVARINADLANDLGNLAQRSLSMVAKQYEGMLPEPGAFSDNDKAI
                     LAQADGMIELARTAMATQQIHHYLNAVWAVVAEANRYFAGEAPWALAKTDPPRQRTVL
                     YVTAEVVRQVAILAQPVMPESSARLLDSLGIPADARDFASLGGATRIEPGTRLPAPVG
                     IFPRY"
     gene            1580..1993
                     /locus_tag="VGA15_18955"
     CDS             1580..1993
                     /locus_tag="VGA15_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589810.1"
                     /translation="MGFFLGLLPAEGVFPATLVLFGVALGLFGPAFFADFLGDFSGSF
                     LAADFFPAVFFAVFFAVFLAGFFTVFLAFFAPRLLVLARFFSAAAFAVRPRGDLRVFF
                     ALRFLTVLLLAVATTISFIAQTRLSGIFAGAASTA"
     gene            complement(1983..>2575)
                     /locus_tag="VGA15_18960"
     CDS             complement(1983..>2575)
                     /locus_tag="VGA15_18960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132145.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the delta' subunit seems to
                     interact with the gamma subunit to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX9589811.1"
                     /translation="PNAANALLKILEEPPQQSLFLLVSHAPARVLPTIQSRCRKLALR
                     PLATSDVISAAAQAANIAADDPALSEAAEASEGSVARALTLLGGDALKLQQRTAALLA
                     TLPRVDPRELHALGDALGGSDRVALGAFIDSVDRWVSQRLRADDANANTNLPRLARLA
                     EVWEKINRAARDTAEYNLERKPLVFSVFGLLSEATR"
BASE COUNT          428 a          801 c          787 g          559 t
ORIGIN      
        1 gtagcgcgga aagattccga ccggcgccgg cagccttgtg cccggctcga tgcgggtcgc
       61 gccacccagg ctcgcgaaat cgcgcgcatc cgcggggatg ccgaggctat ccagcaatct
      121 tgctgacgac tccggcatga caggttgcgc cagaatggcg acctgacgaa ccacttccgc
      181 ggtcacgtac aacacggttc gctggcgcgg cggatcggtt ttcgccagcg cccacggcgc
      241 ctcgcccgca aaatagcggt tggcttccgc cacgacagcc cagacggcgt tcaggtaatg
      301 atgaatctgc tgcgtcgcca tcgccgttcg ggccagctca atcatgccgt cggcctgcgc
      361 caggatcgcc ttgtcgttgt cgctgaaagc gccgggctcg ggcagcatgc cctcatattg
      421 cttggcgacc atcgacagcg agcgttgtgc gaggttgccg agatcgttgg caagatcggc
      481 gttgatgcgc gcgacaatcg cttcgtgatt gtagctgccg tcctgcccga acggcacttc
      541 gcgcaggaag aagtagcgca tctgatcgac gccgtattga ttggcgagat tgaaggggtc
      601 gacgacgttg ccgaccgact tcgacatctt ctcgcccctg ttgaacagga agccgtgggc
      661 atagacgcgc ttctgcagcg gaatgcccgc cgacatcagg aacgccggcc agtacaccgc
      721 gtgaaaacgg atgatgtcct tgccgataat gtgaacgtcg gccggccagt agcgccagtt
      781 cgcattgctc tcgtcgggaa agccgacgcc ggtgatgtag ttggtcaagg cgtcgaccca
      841 gacatacatc acatgctcgg aattgccggg aaccttgaca ccccagtcga acgtggtccg
      901 cgaaatcgat agatcccgca atccgctttt gacgaagctg acaacttcgt tcctgcggga
      961 gtccggacca atgaaatccg gctgatcggc gtagagcgcg agcagcttgt cctgatacgc
     1021 ggacaatttg aagaaatagg tcttctcctc gacccattcg accggtgtgc cctgcggtcc
     1081 gcgccgcaca ttgtcttcgc cgacgacggt ctcatcctcg gcgtaatagg cctcgtcgcg
     1141 cacggaatac cagccggcat agctatccag atagatgtcg ccattgtcgg ccatccgctc
     1201 ccagatctcc cggctggatc gatgatgctg ctcctcggtg gtgcggataa aacgatcgaa
     1261 cgacacgttc agacgctgat ccatctcctt gaacctgagc gcattgcggg tcgcgacttc
     1321 cagcgtcggc agcttttcga gctcggcggt ctggatcatc ttcaggccgt gctcgtcggt
     1381 gccggtcaga aagaacacat ccttgccgtc gagccgctga aaccgcgcca gcgcatcggt
     1441 cgcgatcgcc tcataggcgt ggccgatatg cggcacgccg ttcgggtatg cgatggcggt
     1501 cgtaatgtag aaggactttc gttcagcctg actcggcaag tttgcgacgg gaggcgcttg
     1561 ctttgttgtg gcttcggcct tgggattctt cttggggctc ttgccagccg aaggggtctt
     1621 tccggcaacg ctcgtcttgt tcggtgtcgc gctgggttta ttcggcccag cctttttcgc
     1681 ggatttcctg ggcgactttt cgggaagctt cctcgcagcc gactttttcc ccgccgtttt
     1741 ctttgcagtt ttctttgcag tttttttggc cggcttcttt accgtttttt tggctttctt
     1801 cgccccgcgt ttgctggtct tggcgcgctt tttcagcgcc gccgccttcg cggtgcggcc
     1861 gcgcggcgac ttgcgcgttt ttttcgcgct tcgtttcttg acagttcttc ttttagccgt
     1921 cgccaccacg atttccttta ttgctcaaac gcgactgtcg ggaatattcg ccggcgcagc
     1981 gtctaccgcg tagcttctga aagcagcccg aacaccgaga aaaccagcgg ttttcgttca
     2041 agattatact ctgcggtgtc gcgcgcggcg cggttgatct tttcccacac ctccgccagc
     2101 cgtgcaaggc ggggcaggtt cgtgttggca ttggcatcgt cggcccgcag gcgttggctc
     2161 acccagcgat cgacgctgtc gatgaacgcc ccgagtgcga cccggtcgct gccgccgagc
     2221 gcatccccca gcgcatgaag ttcgcgcgga tcgacccgcg gcagagtcgc cagcagcgcc
     2281 gcggtccgct gctgaagctt tagggcgtcg ccgccaagca gcgtcagcgc gcgcgcgaca
     2341 ctcccctctg aagcctcggc cgcctctgac agcgcgggat catcggccgc gatgttggcg
     2401 gcctgcgccg cggcgctgat gacgtcggaa gtagcgagcg gccgcagcgc cagtttgcgg
     2461 cagcgcgact gaatggtcgg cagcacccgc gcgggggcat ggctgacgag caggaacaac
     2521 gactgctgcg gcggttcctc aagaatcttg agcagcgcat tggccgcgtt ggggt
//
LOCUS       DASWGM010000674         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2686677,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000674 DASWGM010000000
VERSION     DASWGM010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686677"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGA15_18965"
     CDS             complement(<1..239)
                     /locus_tag="VGA15_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589812.1"
                     /translation="MIAPGAIERAKFKSSGPRRDARKRHARLALWAAKSLNGKQRDYG
                     WIIGHGLHLRSGESAKLSVTGKSQEPRGDDSLYNLT"
     gene            complement(346..996)
                     /locus_tag="VGA15_18970"
     CDS             complement(346..996)
                     /locus_tag="VGA15_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX9589813.1"
                     /translation="MIVIRRALAAALSLAAVVAFAPAHLVGDAMAQNATAALVAKPVS
                     LPDMALGPAKAAVTIIEYASMSCPHCAAFEENVFPMLRSKYIDTGKVRFVFREFPIDI
                     KAAAASMLARCIAGDDAEKFFGAVDTLFKQQDRLMDQTKDTLKLIGKQNGLNEPAVES
                     CEKDQALLDKLSADQKFAYEVLKVDATPTFFFNGEMLKGAMSFEELDAKLKALLKN"
     gene            1011..1397
                     /locus_tag="VGA15_18975"
     CDS             1011..1397
                     /locus_tag="VGA15_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589814.1"
                     /translation="MGEGAVKQKPIASSKRKFWQWPPRQRGAGQTNPNDDEPELLRLI
                     GFAGSIGLGLIGLGLIGLGLVGLGLIGFGPRRFLGACRHPGIVFGGTAPRRHTGRLAT
                     HPMRHVRQMLARGLPGARRQQNAGKA"
     gene            complement(1538..2248)
                     /locus_tag="VGA15_18980"
     CDS             complement(1538..2248)
                     /locus_tag="VGA15_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9589815.1"
                     /translation="MTERATIMLVDDHAIVREGYRSLLQKQPRLKVVAEASDGAEAYR
                     LFKATRPDLVIMDLTMPGFGGVEAIRRIRQWDPAARILAFTMHQNAAYAVQAIRAGAR
                     GYVTKSSPPEALVRAIFEVLQGGIAFSADIDHELALSRVADTPAATDVLTPREFEILR
                     MLLAESSTEDIAEILHLSPKTVANTRYLIKAKLGVASDIELVLVALRQGILGESSAAE
                     DASQRARSRAQPKARQDH"
     gene            complement(2245..>3055)
                     /locus_tag="VGA15_18985"
     CDS             complement(2245..>3055)
                     /locus_tag="VGA15_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX9589816.1"
                     /translation="AGDLSARLPPFDLAELSAVGNVFNHLAGSLQTTLSERNALTRRL
                     IVVQDEERQHLARELHDEFGQCLAAISAVAASIGQTAQQECPALMPECQSIARTAAHM
                     MDALRGALLRLRPPDVDDLGLAASLEGLVAGWNTRSGGRTRFSIELSGEFGALPRDFA
                     ASLYRVAQEAITNAAKHAQASRVTLHLRMQEATGADHRAHIEIAVDDDGQAGGDLTLK
                     SGMGLLGMRERITALGGRLSLDTTRPSGLVLCAQIPVPPAAGRTSETRDAA"
BASE COUNT          548 a          938 c          977 g          592 t
ORIGIN      
        1 gttaaattgt aaagagaatc atcgccgcgg ggttcttggc ttttaccggt gactgagagt
       61 tttgcgctct cgcctgatct gagatggagc ccatggccga ttatccagcc gtaatcccgc
      121 tgcttgccat tcaacgactt cgctgcccaa agtgccaaac gcgcatgacg cttgcgcgca
      181 tctcgccggg gcccactgga tttgaacttc gcacgttcga ttgctccagg tgcgatcacg
      241 tcgaaaaaat cgcgatcgcg tcagatccaa tgaagtccgg cgatgtcggc tggctggtca
      301 gcgaattgca gccgccaacg taaggtcagg cccgctggca ggtcgctagt tcttcagcag
      361 cgccttgagc ttcgcgtcca gttcctcgaa cgacatcgcg cctttgagca tctcgccatt
      421 gaaaaagaag gtcggcgtgg catcgacctt caggacctcg taggcgaatt tttgatcggc
      481 ggagagcttg tcgagcagcg cctgatcttt ctcgcagctc tcgaccgccg gttcgttcaa
      541 cccgttctgc ttgccgatca gcttcagggt atctttggtc tggtccatca accggtcctg
      601 ctgcttgaac agcgtgtcga cggcgccgaa aaatttttcg gcatcgtcac cggcgatgca
      661 gcgagccagc atcgaggccg ccgcggcctt gatgtcgatc ggaaattctc gaaacacaaa
      721 gcgcaccttg ccggtgtcga tatatttcga cctcagcatc ggaaaaacgt tttcctcgaa
      781 cgccgcgcaa tgtgggcagc tcatcgacgc gtattcgatg atggtcaccg cggctttggc
      841 cggcccgagc gccatatccg gcagcgacac cggctttgcc accaaggccg ccgtcgcatt
      901 ttgtgccatg gcgtcgccga ccagatgcgc cggcgcgaag gcgacgaccg cggccagaga
      961 cagggcagcg gcaagggcgc ggcgaatcac gatcaactgg gagctccgag atgggcgaag
     1021 gtgcggtcaa acagaagccg atcgctagct cgaaacgaaa attctggcaa tggccgccgc
     1081 gccaacgagg tgccggccag accaacccga acgatgatga gccggaacta ttgagactga
     1141 tcggttttgc cgggtcgatc ggccttggat tgatcggcct tggattgatc ggtcttgggc
     1201 tggttggtct tgggttgatc ggttttgggc ctcgccgctt cctgggcgcg tgccgccatc
     1261 caggcatcgt attcgggggt accgcgccgc ggcggcacac cggcaggctc gccacccatc
     1321 caatgcggca tgtccgccag atgctcgccc gcggtttgcc cggcgcacga cgacagcaaa
     1381 acgcaggcaa ggcatagcac gggccagaga gcccatgtgt gagttgggga gaggcgattc
     1441 attgcggcgt gatgccaacg cattgtggcg atcgcgccgc gtaatcatgg cggggatacg
     1501 gcggtgtggc gagcaggtcg ttcacgacgg cgattcttca atggtcctgc cgcgctttcg
     1561 gctgagcgcg agatcgggcg cgttggctcg catcctctgc tgcgctgctc tcgcccaaga
     1621 tgccctgccg gagcgccaca aggacgagtt cgatgtcgga ggcgacgcca agcttggctt
     1681 tgatgagata gcgcgtattc gccacggtct tcgggctgag gtgaagaatc tcggcgatat
     1741 cctcggtcga gctttccgca agcagcatgc gcaagatctc gaactcgcgc ggcgttagca
     1801 catcggtcgc ggccggcgtg tccgcgacgc ggctcaacgc aagctcgtga tcgatatcgg
     1861 cgctgaacgc tatgccacct tgcaacactt cgaatatggc gcgcaccagc gcttcgggtg
     1921 gactgctttt ggtgacatag cccctggctc ccgcccggat tgcctgcacg gcataggcgg
     1981 cgttttgatg catggtgaag gccagaatgc gggcggcggg atcccactgc cgaatccgcc
     2041 tgatcgcttc gacgccaccg aagcccggca tggtcagatc catgatgacg aggtcaggcc
     2101 gcgtcgcctt gaacaggcgg taggcctcgg cgccgtcgct cgcctcggct acaaccttca
     2161 gccgcggctg cttctgcaac agcgagcgat agccttcccg gacgatcgcg tgatcgtcga
     2221 cgagcatgat cgtggcgcgt tcggtcatgc cgcatccctc gtctcggagg tgcgccctgc
     2281 ggccggaggg acggggattt gagcgcaaag cacaagcccg ctcggacgcg tggtatccag
     2341 gctcaatctg ccgccgagcg ctgtgatgcg ttcgcgcatg ccaagcagcc ccatgccgga
     2401 tttgagcgtg agatcgcccc cggcctggcc gtcgtcatcg acggcgatct cgatgtgggc
     2461 gcgatgatct gcacctgtgg cctcttgcat gcgcagatga agggtgaccc gcgacgcctg
     2521 cgcatgtttt gcggcgttgg tgatggcttc ctgtgcgacc cggtagaggc tcgccgcgaa
     2581 atcccgcggc agcgcgccga attcgcctga cagttcgatt gaaaaacgcg tgcgaccgcc
     2641 actgcgcgtg ttccacccgg caacaaggcc ttcgaggctt gcggcaaggc cgaggtcgtc
     2701 gacatccggc ggccgcagcc gtaaaagagc gccgcgcagc gcgtccatca tgtgggcggc
     2761 ggtgcgggcg atgctctggc actcgggcat cagggccgga cattcctgct gcgcggtttg
     2821 accgatggag gccgcgaccg cgctgatcgc agcaaggcac tggccgaatt cgtcatgtag
     2881 ctctcgcgcc agatgctgcc gttcctcgtc ctggaccacg atcagccgtc gcgtcagcgc
     2941 gttgcgctcc gaaagcgtcg tctgaaggct gccggccaga tgattgaaaa cattgccgac
     3001 ggccgacagt tcggcgagat cgaacggcgg caggcgcgcc gaaaggtcgc cggcg
//
LOCUS       DASWGM010000675         4002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2303741,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000675 DASWGM010000000
VERSION     DASWGM010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303741"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VGA15_18990"
     CDS             complement(<1..182)
                     /locus_tag="VGA15_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX9589817.1"
                     /translation="MIRATKVRSAHRWTEAPADTVVLDFDDRHRRRMAMTGTRGLEFL
                     LDLENAMALRGGDALVL"
     gene            complement(193..492)
                     /locus_tag="VGA15_18995"
     CDS             complement(193..492)
                     /locus_tag="VGA15_18995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX9589818.1"
                     /translation="MIYVVATSQVKPDQRDAFIKGAKECIAATRQEKGCLSYESHTSI
                     NDPNLFVVVERWESREDLNAHGRAPHMKVWREYSAPLKVSPTVIEIISDGKVEKL"
     gene            complement(520..819)
                     /locus_tag="VGA15_19000"
     CDS             complement(520..819)
                     /locus_tag="VGA15_19000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX9589819.1"
                     /translation="MIYVVATLTVKPETRAEFIAGAAACIHETRKEPGNIAYDLHESV
                     TDPSKMVFVEQWENAEALVPHRAAEHMKAFGRVAVRCMSAPPRIEVITPEKVDVR"
     gene            complement(868..2580)
                     /gene="ureC"
                     /locus_tag="VGA15_19005"
     CDS             complement(868..2580)
                     /gene="ureC"
                     /locus_tag="VGA15_19005"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX9589820.1"
                     /translation="MSVKIKRSVYADMFGPTTGDRVRLADTDLIIEVEKDFTVYGEEV
                     KFGGGKVIRDGMGQSQVTNKQGAADTVITNALIVDHWGIVKADVAIKEGFISAIGKAG
                     NPDIQPGVTIVIGPGTDVIAGEGKILTAGGFDSHIHFICPQQIEHALMSGVTSMLGGG
                     TGPSHGTFATTCTPGPWHMARMIQSFDAFPVNLGISGKGNASRPAALVEMINAGACAL
                     KLHEDWGTTPAAINNCLSVADDYDIQVMIHSDTLNESGFVEDTVKAFKGRTIHAFHTE
                     GAGGGHAPDIIKIAGLKNVLPSSTNPTRPFTRNTIDEHLDMLMVCHHLDPSIAEDLAF
                     AESRIRKETIAAEDILHDLGALSMMSSDSQAMGRLGEVIIRTWQTADKMKKQRGALPQ
                     DKGNNDNFRVKRYIAKYTINPAIAHGVSKLIGSVEKGKLADLVLWSPAFFGVKPERII
                     KGGSIVAAPMGDPNASIPTPQPVHYQPMFAAYGKSLTASSVVFSSKAAVSSGLAKKLG
                     IDKKLYAVENTRGGISKKSMIHNGATPKIEVDPETYEVRADGELLTCAPAEVLPMAQR
                     YFMF"
     gene            complement(2683..2988)
                     /locus_tag="VGA15_19010"
     CDS             complement(2683..2988)
                     /locus_tag="VGA15_19010"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX9589821.1"
                     /translation="MIPGELFIQDGEIELNAGRKTLTLTVANSGDRPIQVGSHYHFFE
                     TNPALKFDRKKARGMRLDIAAGTAVRFEPGQTRDVQLVALAGKRVVYGFRGEVMGKL"
     gene            complement(3132..3434)
                     /locus_tag="VGA15_19015"
     CDS             complement(3132..3434)
                     /locus_tag="VGA15_19015"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX9589822.1"
                     /translation="MNLSPREKDKLLISMAAMVARRRLERGVKLNHPEAIAIISDFIV
                     EGARDGRTVAELMQAGAEVITRAQCMDGIAEMIHDIQVEATFPDGTKLVTVHEPIR"
     gene            complement(3510..>4002)
                     /locus_tag="VGA15_19020"
     CDS             complement(3510..>4002)
                     /locus_tag="VGA15_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX9589823.1"
                     /translation="HLAWLPQETILFDRARLKRRIDIDLAEGASLLLCEIVVFGRAAM
                     GERMRQGEFVDRWRMRRGGKLVFAETIRLDGDIGEQLARPAIAKGGVAIGTALIVPGD
                     EALVERIRELSKSFGSEVGISCWNGFAMARFCAQDAARLRADMMAVLGRASGLALPRL
                     WLS"
BASE COUNT          715 a         1233 c         1242 g          812 t
ORIGIN      
        1 agcaccagcg cgtcgccgcc gcgcagcgcc atcgcgtttt ccagatcgag caggaactcc
       61 aatccccgcg tgccggtcat cgccatccgc cgccggtgac ggtcgtcgaa atcgagcacg
      121 acggtatcgg ccggcgcctc cgtccagcga tgggcgctgc ggaccttggt ggcgcggatc
      181 atgaaaaaat gctcacagct tctcgacctt gccgtcgctg atgatctcga tcacggtcgg
      241 cgacactttt agcggcgcgg aatattcgcg ccagaccttc atgtgtggcg cccggccatg
      301 ggcattgagg tcctcgcggc tttcccagcg ttccaccacc acgaaaagat tgggatcgtt
      361 gatgctggtg tgactctcat aggagagaca gcctttttcc tggcgggtcg cggcgatgca
      421 ttccttggcg cccttgatga aggcatcccg ctggtccggt tttacctgag aggtggcgac
      481 gacgtagatc atggtttggg tctcccgctg gtttcttgat tatctgacgt cgaccttctc
      541 gggcgtaatc acctcgatcc ggggcggcgc cgacatgcat ctcaccgcga cacggccaaa
      601 cgccttcatg tgctcggcag cgcgatgcgg caccagcgcc tcggcgtttt cccattgctc
      661 gacgaacacc attttggagg gatcggtgac gctctcatgc aggtcatagg cgatattgcc
      721 aggctccttg cgggtttcat ggatgcaggc ggcagccccg gcaatgaact cggcgcgggt
      781 ttcaggcttc acggtcagtg tggcaacgac gtagatcacg atatcctccc ggtcttttgg
      841 tctggcttga gtgtgccggg agtgagctta gaacatgaaa tagcgctgcg ccatgggcag
      901 cacctcggcc ggcgcgcagg tcagcaattc gccgtcggcg cggacctcat aggtctcggg
      961 atcgacctcg atcttgggcg tggcgccgtt gtggatcatg ctctttttcg agatgccgcc
     1021 acgggtattt tcgaccgcgt agagtttctt gtcgataccg agcttcttgg ccagccccga
     1081 agagaccgcc gccttggagg aaaacaccac cgacgaggcg gtgagagatt tgccataggc
     1141 ggcgaacatc ggctggtagt gcaccggctg cggcgtcgga atcgaggcgt tgggatcgcc
     1201 catgggtgct gcgacaatgg agccgccctt gatgatgcgt tcgggtttga cgccgaagaa
     1261 ggccggcgac cacagcacca gatcggcgag ttttcctttt tccaccgagc cgatcagctt
     1321 tgagacgcca tgggcgatcg cggggttgat ggtgtatttc gcgatgtagc gcttgacccg
     1381 gaaattatcg ttgttgccct tgtcttgcgg cagcgcgccg cgctgcttct tcatcttgtc
     1441 ggcggtctgc caggtccgga tgatgacttc gccgagccgg cccatggctt gcgaatccga
     1501 cgacatcatc gacagcgcgc cgagatcatg caggatgtct tccgccgcga tggtctcctt
     1561 gcggatgcgg ctttcggcaa acgcaagatc ctcggcgatc gagggatcga gatggtggca
     1621 gaccatcagc atatccagat gctcgtcgat ggtgttgcgg gtgaacggcc gcgtcggatt
     1681 ggtcgaggac ggcagcacgt ttttcaaacc cgcgatcttg atgatgtcgg gcgcgtggcc
     1741 gccgccggcg ccttcggtat ggaaggcgtg aatggtgcgg cccttgaagg ccttgacggt
     1801 atcctcgacg aagcccgact cattcaacgt gtcggaatgg atcatcacct ggatgtcgta
     1861 atcgtcggca acggaaaggc agttgttgat cgcggccggc gtggtgcccc agtcctcatg
     1921 cagcttcagc gcgcaggcgc cggcgttgat catctccacc agcgccgcag gtcgcgaggc
     1981 gttgcccttg ccggaaatgc cgagattgac cggaaacgca tcgaacgact ggatcatccg
     2041 cgccatgtgc caggggccgg gcgtgcaggt ggtcgcaaac gtgccgtgcg aggggccggt
     2101 gccgccgccc agcatcgagg tgacgccgga catcaacgcg tgctcgatct gctgcgggca
     2161 gatgaaatgg atgtggctgt cgaatccccc cgcggtcagg atcttgccct cgcccgcaat
     2221 gacgtcggtg ccgggaccga tgacgatggt gacgccgggc tggatgtcgg gattgcccgc
     2281 cttgccgatg gcgctgataa aaccttcctt gatggcgacg tcggccttga cgatccccca
     2341 atgatcgacg atcagcgcat tggtgatgac ggtatcggcc gcaccctgct tgttggtgac
     2401 ctgggactga cccatgccgt cgcggatcac cttgccgccg ccgaatttca cctcctcgcc
     2461 atagaccgtg aaatcctttt cgacctcgat gatcaaatcg gtgtcggcga gccgcacccg
     2521 gtcgccggtg gtcggcccga acatgtcggc atagacggaa cgttttattt tcacggacat
     2581 cagctgtgct ccggattaca ggtcgtcatc cccggatgag cgcaattgcg ctcatccggg
     2641 gatgacgccg gcgtgtatgg cggatggcta taggaacgtg gctcacagtt ttcccatcac
     2701 ctcgccacga aaaccgtaga cgacgcgctt gccggccagc gccacgagtt gcacgtcgcg
     2761 ggtttggccc ggctcgaagc ggaccgcggt gccggcggcg atatcgagcc gcatgccgcg
     2821 ggccttcttg cggtcgaact tcaatgcggg attggtctcg aagaaatgat aatgcgagcc
     2881 gacctggatc ggacggtcgc cggaattggc gacggtcagc gttagggtct tgcggccggc
     2941 attgagttcg atctcgccgt cctggatgaa taattcgccg gggatcatcg tgcgctctct
     3001 cctcagtcat tccggggcgc gtcgaagacg cgaactatga tgtgcaattg cacatcggag
     3061 aatctcgaca ttagtaacct ctggattccg ggtctgcgcc tgcggcgcat cccggaatga
     3121 ctccctgaat ctcatcggat cggctcgtgc acggtgacga gcttggtgcc gtcgggaaac
     3181 gtcgcttcga cctggatgtc gtggatcatc tcggcgattc catccatgca ctgcgcgcgg
     3241 gtgatgacct ctgcgccggc ctgcatcagt tcggccacgg tgcggccgtc gcgcgcgccc
     3301 tcgacgatga aatcggaaat gatcgcgatg gcttccggat ggttgagctt gacgccacgc
     3361 tccagccgcc gtcgcgccac catcgccgcc atcgagatca gaagcttgtc cttttcgcgg
     3421 ggggacagat tcatgcgagc actctaatca ttttgaacca tctaattgaa ccacaaccta
     3481 actaagccac agcctaactg agccaaagcc taactgagcc aaagccgcgg cagcgccaaa
     3541 ccagaggcgc ggccgagcac cgccatcata tcggcacgaa gcctggccgc atcttgggca
     3601 cagaagcgcg ccattgcaaa cccattccag caggagatcc cgacctcgct cccgaacgac
     3661 tttgacagtt cgcgaatccg ctccaccagc gcctcatcgc cgggcacgat cagcgccgtg
     3721 ccgatggcca cgccgccctt cgcaatcgcg ggcctcgcca gctgttcgcc gatgtcgccg
     3781 tcgagccgga tggtttcggc gaacacgagt ttgccgccgc gccgcatccg ccagcggtcg
     3841 acgaattcgc cttgccgcat tcgctccccc atcgcggcgc ggccgaatac cacgatctcg
     3901 cagagcagca gcgaggcgcc ctccgcaagg tcgatatcga tccggcgctt tagccgcgcc
     3961 cggtcgaaca gaatggtctc ctgcggcagc caggcgagat gc
//
LOCUS       DASWGM010000676         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2444150,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000676 DASWGM010000000
VERSION     DASWGM010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2444150"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1663
                     /locus_tag="VGA15_19025"
     CDS             92..1663
                     /locus_tag="VGA15_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9589824.1"
                     /translation="MTNEKVDVVIVGAGASGSVYASVLAKAGKKVVLIESGPDWELSD
                     LISSEIWGRRVKPAGGPILLEGKNPMGYANQAGWGVGGAALHYFANFPRLLPTDFKVK
                     SEHNRAQDWPISYQDVAPFYDKVAREIGVSGDAKAEQRWRPAGEAYPMPPMKTFRSGN
                     IFLKGFEASGIPMVPAPVGMNSVEFKGRPACLYDGWCHVGCPIGALANPLVTYLGDAR
                     KAGAEVRARSTVTRILTNQAGTRVTGVEYYDDKQQKQTQEASVVLLAAWSAQNPRLLL
                     NSATDKHAKGLGNASGLVGRFMMTHHVASTWAMFDEDVQNHMGTIAVQFMSYERYAKT
                     SHPGAFGSSFVTAGFALKTSDLANSRGDLFGPELADYMKRAARNLAGIKTFGEDQPEM
                     ENRVELTSAKDEFGMPLGKLIHSYGQDPLALWNANLEEGLKIAKASGAKEAWSARGGA
                     IPTSHLMGGTIMGSGAGDSVVDSYGQSHEIPNLWVAGPSIFPTAGASNPTFTIFALSQ
                     RGAERMASQWATLTN"
     gene            1858..>2857
                     /locus_tag="VGA15_19030"
     CDS             1858..>2857
                     /locus_tag="VGA15_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9589825.1"
                     /translation="MAAQGYDYIIVGAGSAGCVIANRLSADPSCCVLLLEAGGSDRDF
                     WLKLPIGYYRTIFNERFSRLFVTEPSERTGGRSIVWPRGRVLGGSSSINGLIFIRGQH
                     EDFDDWERLGAKGWSYRELLPYFRRYERYRGGESQYHGGLGEFRVSDLRTGNRASAAW
                     VEAGVEFGLPRNPDFNGATTLGVGAYQLGIGRHWRSSSASAFLRPVAHRPNLTVITDA
                     QVSKVVLKGRAATGVEWISNGKVFGAAADREVVLSAGALQSPQLLQLSGVGPADLLRG
                     LGIPVVVDAPEVGRNLQDHYQARMIVRLKQPISLNDQVRNPYELAKMGLQWMLAGSG"
BASE COUNT          557 a          817 c          925 g          558 t
ORIGIN      
        1 gccgtcgacg tcgtctatgg cacgatggaa ggctacgagg cgctgggcat cccctatatg
       61 ccgcatatcc cgccagaaaa gaggtggtga gatgacaaac gagaaagtgg atgttgtcat
      121 cgtcggggcg ggcgcttccg gttctgtcta tgcatcggtg ctggcgaagg cgggcaagaa
      181 agtcgttctg atcgaatctg gccccgactg ggagctgtct gatctcatca gttccgagat
      241 ctggggccga cgggtcaagc ccgcgggcgg gccgatcctt ctcgaaggca agaatccgat
      301 ggggtacgcc aaccaggccg gctggggcgt cggcggtgcg gcattgcact acttcgccaa
      361 ctttccacgg ctgctgccga ccgatttcaa ggtcaagagc gagcacaacc gggcacagga
      421 ctggccgatt tcctatcaag acgtcgcgcc gttctatgac aaggtcgcgc gcgagatcgg
      481 tgtttccggt gatgcgaagg ccgagcagag atggcggccg gcgggagaag cctatccgat
      541 gccgccgatg aagaccttcc ggagcggcaa tatctttctg aagggcttcg aagcctccgg
      601 gattcccatg gtgccggcgc cggtcggcat gaattcggtg gaattcaagg gacgccccgc
      661 ctgcctgtat gacggatggt gtcacgtcgg ctgtccgatc ggtgcgctcg ccaatccgct
      721 ggtcacctat ttgggcgatg cgcgaaaggc tggcgccgaa gttcgtgctc ggtccacggt
      781 gacacggatt ctgaccaatc aggccggtac gcgcgttacc ggcgtcgaat attacgacga
      841 caagcagcaa aagcagaccc aggaagcgag cgtggtgctg ctggcagcat ggtcggcgca
      901 aaatccgcgg ctgttgctga actccgcgac cgacaagcac gccaagggcc ttggcaatgc
      961 gagcggactc gtcggccgct tcatgatgac gcaccacgtc gcgtcgacct gggcgatgtt
     1021 cgacgaggat gttcaaaatc acatgggcac gattgccgtg cagttcatgt cctacgagcg
     1081 ttatgccaag acgagccacc cgggcgcgtt cggaagttct ttcgtcaccg cgggcttcgc
     1141 attgaagacg agcgatcttg ccaactcccg tggcgattta tttgggccag aactcgccga
     1201 ttacatgaag cgcgcggcgc gcaatctggc ggggatcaag accttcggcg aagaccagcc
     1261 ggaaatggaa aaccgcgttg agctgacaag tgccaaggac gagttcggca tgccgcttgg
     1321 aaagctgatc catagctacg gtcaggatcc gctggcgcta tggaatgcca atctcgaaga
     1381 gggattgaag attgccaagg cgagcggagc caaagaagcc tggtcggcgc gtggtggagc
     1441 gatcccgacc agccatctga tgggcggcac catcatgggg tcgggggctg gcgattctgt
     1501 cgtcgatagc tatggccaaa gccacgaaat tccgaatctc tgggtcgcgg gaccaagcat
     1561 ctttccgacc gcgggtgcgt cgaatccgac gtttacaata tttgcattgt cgcagcgcgg
     1621 tgccgagcgt atggcgtcgc agtgggcaac gctgacgaat tagcaggaag cccgatccgc
     1681 ggtacttgcc tgggtcgcgc gcgcgtcagc accggccttg ctttcgcttg cccgacaagg
     1741 atgttgccca cgctcaaaaa gcgacatcga gggaacgtcc agttttggcg ccaaaagcgg
     1801 actttcgccg aaaagctaac gtcagctgag cgctaaatga ggaaggtgtc tgcttgcatg
     1861 gctgctcaag gttatgacta catcatcgtc ggtgcggggt cggccggctg tgtgatcgca
     1921 aaccggctgt cggccgatcc atcatgctgc gtgctgctgc tggaggccgg ggggtccgat
     1981 cgcgattttt ggctcaagct cccgattgga tactaccgga cgattttcaa tgagcgcttt
     2041 tcccgcctct tcgtgactga gccgagcgag cgcaccggcg gccgctcgat tgtttggccc
     2101 cgcggtcgtg tgctgggcgg atcgtcgtcg atcaacggcc tgatcttcat tcgtggtcag
     2161 cacgaggact tcgacgattg ggaacgactc ggtgccaaag ggtggagtta tcgcgagctg
     2221 ttgccgtatt tccgccgcta cgaacgttac cgcggcggcg agagccaata tcacggcggc
     2281 ctcggcgaat tcagggtttc cgaccttcgt accggcaacc gcgcatccgc ggcatgggtg
     2341 gaagccggcg tcgagtttgg attgccgcgc aatccagact ttaacggcgc gaccacgctc
     2401 ggcgtcggcg cgtaccagct cggcattgga cgacactggc gcagcagctc ggcgtcggct
     2461 ttcttgcgtc ccgtcgcgca tcgcccgaac ctgaccgtca tcaccgatgc gcaagtcagc
     2521 aaagtggtac tcaagggtcg cgccgcaacc ggcgtggaat ggatcagcaa cggcaaggta
     2581 ttcggtgcgg cggccgatcg cgaagtcgtg ttatcggccg gcgcgctaca gtcgccacaa
     2641 ttgctgcaac tgtcaggcgt cggtccggcg gatctgctgc gtggacttgg cattcccgtt
     2701 gtcgtcgacg cgccggaggt cggccgcaat ctgcaggatc actatcaggc tcgcatgatc
     2761 gtccggctga aacagccgat atcgctgaac gaccaggtcc gtaatcccta cgaacttgcc
     2821 aagatgggct tgcaatggat gctggcaggc agcggtc
//
LOCUS       DASWGM010000677         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1577697,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000677 DASWGM010000000
VERSION     DASWGM010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577697"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VGA15_19035"
     CDS             <1..264
                     /locus_tag="VGA15_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="keto-hydroxyglutarate-aldolase/keto-deoxy-
                     phosphogluconate aldolase"
                     /protein_id="HEX9589826.1"
                     /translation="GFNLVKFFPAEQSGGIKALRALAGPFPDARFCPTGGIGEANAVA
                     WLAEPNVSAVGGSWLCPAADIRSGNWAGITAMCDRTMKLLKPA"
     gene            315..1484
                     /locus_tag="VGA15_19040"
     CDS             315..1484
                     /locus_tag="VGA15_19040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX9589827.1"
                     /translation="MTASIVGWAHTPFGKFDTETVESLVVKVATDALADAGISAADVD
                     EIVLGHFNAGFSPQDFTASLVLQADPHLRFKPATRVENACATGSAAVHQGVRAIAAGA
                     AKIVLVVGVEQMTRTPAAEIGRNLLRASYLPEDGDTPGGFAGVFGKIAQGYFQKYGDQ
                     SDALAMIAAKNHKNGVANPYAQMRKDVGYEFCRSESEKNPFVAGPLKRTDCSLVSDGA
                     AALVLTDAETAKTMGKAVNIRATAHAQDFLPMSKRDILLFEGCTVAWQRALQSAGVQL
                     SDLSFVETHDCFTVAELIEYEAMGLTPRGQGARAIKEGWTQKDGKLPINPSGGLKAKG
                     HPIGATGVSMHVMSAMQLLGQAPEGMQIKNAKLAGIFNMGGAAVANYVSVLEPAK"
     gene            1977..3242
                     /locus_tag="VGA15_19045"
     CDS             1977..3242
                     /locus_tag="VGA15_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589828.1"
                     /translation="MKFFWSWQSDTPGETGRYFVRDSLLAAIKQLRQPEDVEEPTSAA
                     HREAMELTSDRQGVTGSPPLAATIKRKINECEVFVADITPVSKVPRRKGVKEKRNMNI
                     NVGIELGYAIRAHGEEQILLVLNEHYGGREFLPFHLQDLGGPITYHLRHDATKEQIDT
                     ELSKLRGKFVTGIREFLKKAGPAQEAKSTFRRVPSTTSIAAWYAHGETVADFDSETSY
                     AFSDDKGVYLRVSPRTPAPRPFTSGQLLSVARRRQMGLLWRTQAGFAHGNTKGAVVIE
                     PVSGNGGRVHAASQVFHSGEIWGIGRDLLVDNDHGKFIAVWRLEEAMRYGLEGFVEFI
                     GQLGLPPPYDVEFGVVGTKGYSLVIEQTYDNPYVIYEDTYTDSLILQSTTPEVIDEAL
                     LMIFESFFRMTGHPRPPHLFDFPPKRRGG"
BASE COUNT          739 a         1101 c         1170 g          734 t
ORIGIN      
        1 ggtttcaacc tggtgaaatt cttcccggcc gagcagtccg gcggtatcaa ggcacttcgg
       61 gccctggccg ggccttttcc ggatgcgcgg ttttgtccga ccggcggcat cggcgaggcc
      121 aacgctgtcg cgtggctggc cgagccgaac gtatcagcgg tgggcgggtc ctggctatgc
      181 ccggcggcgg atatcaggtc cggcaattgg gctggcataa ccgccatgtg cgatcggacc
      241 atgaaattgc tgaaaccggc gtgaagtata tccgccgata agttacaaac cctccgagga
      301 agaaggagaa caccatgaca gccagcatcg tcggatgggc gcacacgccg ttcggcaagt
      361 tcgataccga gactgtcgag agcctggtgg tgaaggtcgc caccgacgcg ctggcggacg
      421 ccggcatctc cgctgccgat gtcgatgaaa tcgtgctcgg gcatttcaac gccggcttct
      481 cgccgcagga tttcaccgca tcattggtgc tgcaggccga tccgcacctg cgcttcaagc
      541 cggccacacg ggtcgagaat gcctgcgcga ccggctcggc ggcggtgcat cagggcgtca
      601 gggccattgc ggccggtgct gcgaagatcg tgctggtggt cggcgtggag cagatgacgc
      661 gcacacccgc agccgagatc ggccgcaacc tgctgcgcgc gtcgtatttg ccggaagacg
      721 gcgacactcc cgggggattc gccggcgtgt tcggcaagat cgcgcaaggc tatttccaga
      781 aatacggcga ccagtccgat gcgctggcga tgatcgccgc caagaaccac aagaacggcg
      841 tcgccaaccc ctatgcgcaa atgcgcaagg atgttggcta cgaattctgc cgctccgaaa
      901 gcgagaagaa cccgttcgtg gcagggccct tgaagcgcac cgattgctcg ctggtgtcgg
      961 atggcgcggc ggcgctggtg ctgaccgatg ccgaaaccgc gaaaaccatg ggcaaggccg
     1021 tcaacatcag ggccaccgcg catgcccagg attttctgcc gatgtcgaag cgcgacatcc
     1081 tgctgttcga aggctgcacc gtggcatggc agcgcgcgct gcaatccgcc ggcgtgcagc
     1141 tttcggatct ttcctttgtc gaaacccacg actgcttcac cgtcgccgag ctgatcgaat
     1201 atgaggcaat gggcctgacg ccgagggggc agggcgcccg cgccatcaag gaaggctgga
     1261 cccagaagga cggcaagctg ccgatcaatc catccggcgg attaaaggcc aagggccacc
     1321 cgatcggcgc caccggcgtc tccatgcatg tgatgagcgc catgcaactt ttgggtcagg
     1381 cgcccgaggg catgcagatc aaaaatgcaa aactcgcggg catcttcaac atgggcgggg
     1441 cggcggtcgc caattacgtt tcggtgctgg agccggcgaa gtaggcgctc gaaaaaaact
     1501 ccatcgtcgc cccggcgaac gccggggccc ataccgcgtt gtcccatcaa tagggcaccg
     1561 gcggcagaga ccgacaactg gcgccggtgg ctatggtgcc tgcttttcgc agggacgacg
     1621 tctacgtctt ggtttagcaa cgtgagcaaa ggcgcgcttg cgccgtgcca ccaatgttcg
     1681 gcgagagcgg aacgtaggat cttgttcggc aatattggcc tgatagccgc tcgttcgtgg
     1741 tctgcgaatt cgtcgcgtat tgatttcgaa acgccagtct cggcagcttt gaacaacgcc
     1801 tgtgccgatg cctgcaacct cgcgctcggc ccttccgata ttgcccgata accggacgcg
     1861 gcccagttgc gcgaaaatta acgcgaatga cccgcaaccg accttaggtc gttgttaggt
     1921 cgcagccttg tcgttatgct cgccacgggt cctttgaggt agattctcat ccgcgcatga
     1981 agtttttctg gtcatggcag tcagatacgc ccggcgagac cgggcggtat ttcgtgcgtg
     2041 actcgctgct cgccgccatc aaacaattgc gccagcccga ggacgtggag gagccaacca
     2101 gcgctgccca tcgcgaggcc atggagctca cttccgaccg ccagggtgta acaggaagcc
     2161 cgccgcttgc ggcgacgatc aagaggaaga tcaatgaatg tgaggtcttt gtcgccgata
     2221 tcactcccgt ttccaaggtc ccccggcgca agggcgttaa ggaaaagcgg aacatgaaca
     2281 tcaacgtggg catcgagctt ggctacgcga tccgcgccca cggtgaggag cagatcttgc
     2341 tagtcctcaa cgagcactat ggtggccgtg aattcctgcc gttccatctc caagatcttg
     2401 gcggaccgat tacttaccac ctccggcacg acgcgactaa ggagcagatc gataccgagt
     2461 tgtcgaaact gcgcgggaag ttcgtgacag ggatccgtga attcttgaag aaggccggtc
     2521 ctgcccaaga ggccaagtca actttccgac gagttccctc tacgacgtca attgctgcct
     2581 ggtatgcgca cggcgagaca gtcgccgatt tcgacagcga gacttcatat gccttttctg
     2641 acgacaaggg cgtatatctc cgagtttcac cgcggacgcc ggcgccgcgg ccgttcacca
     2701 gtggtcagct gctctccgta gcgcgtcggc gtcagatggg cctgctttgg cggacgcagg
     2761 ccggcttcgc gcatggcaac accaagggtg ccgtggtgat tgagccagtc agtggaaacg
     2821 gcggcagggt acatgctgca tcgcaggtct tccacagtgg cgagatctgg ggcatcggcc
     2881 gggacctttt ggttgataat gaccacggta aattcatcgc cgtttggcgc ctcgaagagg
     2941 caatgcgcta tgggttggag ggctttgttg aattcatcgg gcaactcggc ctgccgccgc
     3001 cctatgatgt cgagttcggc gtcgtaggca ctaagggcta ctcactcgtc atcgagcaga
     3061 cttatgacaa tccctatgtg atttacgaag acacttatac tgacagcctg atcctgcaga
     3121 gcacaacgcc cgaggtgatc gatgaggcat tgctgatgat ctttgaatct ttcttccgga
     3181 tgacgggcca tccgcgtccg ccccacctgt tcgattttcc ccccaagcgc cggggcggct
     3241 aagtacggaa acccgaacta atgagggtag aagtttcaga tagaattccg ttgacgggtg
     3301 agtgattgcg caagccccaa gcttccgaaa gccattgccc gtcgggcaaa tcggttgaat
     3361 ttccagacct cgtctccgtc gctgcggcct ttgagccgcc aaaatcgcgt gcgcgaaaaa
     3421 tccgatttcg cgtggccgtt caagctgatt tgggtggtcc agtcctgcgc gcgaaaataa
     3481 ttcgctttcg ttttttccga aattggcggc tactgctccc gtcctgcctc ggcaagaggg
     3541 gcgtacgcgt cgtcacgaac gcggggcggg atgcggtgga cgcggcagcg tcaggcgtgc
     3601 gatgcgaagc gcagggcgga ttgctctccg tgagcggtta gcggcatgca agacgacggc
     3661 gctgcttgcg gacggtgaag tcgtgtggtc ctgacgcctc gacgctggcg tcaagtcttg
     3721 cggaggctcg ttcggctcaa ccgg
//
LOCUS       DASWGM010000678         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2303984,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000678 DASWGM010000000
VERSION     DASWGM010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303984"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VGA15_19050"
     CDS             complement(<1..964)
                     /locus_tag="VGA15_19050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9589829.1"
                     /translation="MAGRFLRELTIRQLRALAAVQKHRSVTAAAKQLHLTQPAVTLQL
                     RNLQALAGLPLIQRTSDGMLLTDAGREVLALSERIEAAIASCETSLDMIAGKTAGRIS
                     IGAVSTAKYFVPFAISGFSRAYPNVDVTLSIGNRQEIGTALRGYDLDIAIMGRPPVDI
                     PMNVHLIGDHPHVIIAPTAHRLARKARLSLSALSDETFLTREPGSGTRGLMEQLFEQA
                     GVRPKIGMAMSSNETIKQAVIAGLGIAFISAHTVATELDERRLVTLDVVGLPVVRQWF
                     VLSRKDKVLLPPAQAMLDFLSAKGAQFLPRTHGRLRLTRPSAPAK"
     gene            1111..>1556
                     /locus_tag="VGA15_19055"
     CDS             1111..>1556
                     /locus_tag="VGA15_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX9589830.1"
                     /translation="MDERVTLRSHLDRSTSQTPHGVAVGEAIAAIAAAAVDLAALIAD
                     GPLAGITGRSGGTNADGDQQKDIDVAADEMMRRALREAPVAAILSEEVTLPEIINSEA
                     PLCVAIDPLDGSANLENNISVGTIFSIRPKGNDILSTFFEPGTAQCA"
BASE COUNT          314 a          479 c          480 g          283 t
ORIGIN      
        1 gcttggcggg tgctgatggc cgggtgaggc gaagcctgcc gtgggtgcgc ggcagaaatt
       61 gagctccctt ggcgctgaga aagtcgagca tggcctgcgc gggaggcagc agcaccttgt
      121 ccttgcggga aagcacaaac cattgccgga ccaccgggag accaacgacg tccagcgtca
      181 ccagccgtcg ctcgtcgagt tcggttgcga ccgtatgcgc tgaaataaat gcaatgccca
      241 aaccggcaat caccgcctgt ttgatggtct cgttgctgct catcgccatg ccgattttcg
      301 ggcgaacacc ggcctgttcg aacagttgct ccatcaaacc gcgtgttccg gatccgggct
      361 cgcgtgtcag gaacgtttca tcggaaaggg cactcagcga cagacgcgct ttccgcgcca
      421 gccgatgcgc ggtcggcgcg atgatgacat gggggtgatc gccgatcaga tgaacgttca
      481 tggggatatc caccggcgga cgtcccatga tggcgatatc cagatcgtag ccgcgcaacg
      541 cggtgccgat ttcctgccgg ttgccgatcg agagtgtgac gtcgacgttc ggatacgccc
      601 gcgaaaatcc ggagatcgcg aacggcacga aatatttcgc agtactgacg gcgccgatgg
      661 aaatacgccc ggcggtcttt cccgcgatca tatcgagcga ggtttcacag gacgcaattg
      721 ccgcttcgat tcgctcggaa agcgccagca cttcgcgccc cgcatcggtc agcagcattc
      781 cgtcgctggt acgctggatc agcggcagtc ccgccaacgc ctgcaagttg cgaagctgaa
      841 gggtgaccgc gggctgggta agatgaagtt gcttggctgc cgcggtcacc gaccgatgtt
      901 tttgaacggc cgccaacgcc cgcaactggc ggatggtcaa ttcccgcaaa aaccggcccg
      961 ccatcgcgat cacctttata agaaaaactt tgctcagtcc aaagataaca aaatttcact
     1021 aatccggcaa attgcgcttc tctgaatggg cggaccgccg accggcgaaa cgcagtggga
     1081 gcgccccgaa gagggcccag ggagacggcc atggacgagc gcgtgacctt gcgttcgcat
     1141 ctcgatcggt ccacgtcgca aacgccgcat ggcgttgcgg tgggcgaggc gatcgcggcc
     1201 attgctgccg ccgcggtcga ccttgccgcc ctcatcgcgg acgggccgct ggcgggcatc
     1261 accggccgga gtggcggcac caatgccgat ggcgatcagc aaaaggatat cgatgtcgca
     1321 gccgatgaaa tgatgcgtcg cgcgctgcgg gaggccccgg tcgctgcgat tctttccgaa
     1381 gaggtgacgc tgccggaaat catcaattcc gaggcgccac tctgcgtcgc catcgatccg
     1441 ctcgacggat cggccaacct tgaaaacaac atctcggtcg gcaccatctt ttcgatccgc
     1501 cccaagggca acgacatcct ctcgaccttc tttgaaccgg gcacggcgca gtgcgc
//
LOCUS       DASWGM010000679         9591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2161616,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000679 DASWGM010000000
VERSION     DASWGM010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161616"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VGA15_19060"
     CDS             <1..684
                     /locus_tag="VGA15_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9589831.1"
                     /translation="GGLDGQGQKSLLLALFGVLRGVTGRITVEGREVRPGSPAAAKSP
                     AVGIALVPEDRKTEGLMLPMSIADNLAIASLDAISTGPFVDRSKERAAVERGIARLQI
                     KIGAPGDAVSTLSGGNQQKVVIAKWLMTEPRIILLNDPTRGIDVGTKQELYRLMRELA
                     DQGAAILFYSTDYDELIGCCDRVAIMYDGRIVRELEGSELTETNIIASALNIDAATSA
                     FAAAGAAHA"
     gene            677..1789
                     /locus_tag="VGA15_19065"
     CDS             677..1789
                     /locus_tag="VGA15_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589832.1"
                     /translation="MLDDLAIRLRQNIGFVIAILLVCTSYLLYNLAHPKGFSSAVLVQ
                     NGDEIFALAMLAMAQTVPVLMSGLDLSVGAVMTMVGCFASYLLAGTAGGTPLHLEIFG
                     LYLGLGTFPGGTSGILLGIAACVAIGTLAGFINGCVVVYGRIQPIIATLATGAVYIGI
                     ALFLRPTPGGKIDEDLNWALTNSLGDFASTVHIFDDGAAGWFAPFAWIPVPFVLLVLI
                     ALFVWMPFRRSVLGRAVYAVGSAEGAAYMSGLPIDRAKIAAFTLAGFFAGCGGLFLAI
                     QTSSGNADIPQAGAYTLNSIASVVIGGTSLLGGTGSAIGSIFGAMILRVISFFFRIFD
                     IAPLLQPLFEGVILLVAVSIGALGVLRVRNTLELFR"
     gene            1795..2745
                     /locus_tag="VGA15_19070"
     CDS             1795..2745
                     /locus_tag="VGA15_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589833.1"
                     /translation="MSLGLSRDDRPILIAALFILVILVAGTTYTIARFGSAPLLSPTY
                     LLQQLQIGSFLGIVAAGMMIVILIAQIDLSVPWTLAAAAMMATSIGGPLAIPVGLGIG
                     LLVGLVNGIGVAYLRVPSMIFTLGVNAVMRGLMVAHTGGYAPQTTATELMRTLAAERT
                     LGIPNALFVWAAVSVLVVVILQRTPLGRYIYAIGNKEAAAYLAGVNTRRVTVICFVLC
                     GLAAALAGVLLAGYSTKAYQAMGDAYLLPSIAAVVIGGTNILGGRGRYLGTLVGVVLI
                     VLLNSVLSIMDMPEAGRQVIYGLVIIQMLLVYGRGERITS"
     gene            complement(2765..3696)
                     /locus_tag="VGA15_19075"
                     /pseudo
     CDS             complement(2765..3696)
                     /locus_tag="VGA15_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004670754.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
     gene            complement(3912..4094)
                     /locus_tag="VGA15_19080"
     CDS             complement(3912..4094)
                     /locus_tag="VGA15_19080"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX9589834.1"
                     /translation="MQTEHVVMFEAIEARDKDAARLAMRTHIEDACMRIFEGPGGCAS
                     RKGEPKNMSKSADVSR"
     gene            4080..4733
                     /locus_tag="VGA15_19085"
     CDS             4080..4733
                     /locus_tag="VGA15_19085"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589835.1"
                     /translation="MFGLHGSQMFERPCKREATREIDREQAGRSDGQQIMVRNGYAYI
                     GHVCSNGFSVVDVRDPTRPKTVNCVQNPPNTWSLHLQVHDDLLLLVHGRDMFSQPEMA
                     GERNYYKPKSGTHGYVEAPRARNWSAGMAVFEISKPAEPRQIGFMPVDGVGLHRIWYV
                     GGRWAYASAMMEGFSDYILITIDMQDPAKPQIVGRYWLPGMNLAAGETANWPLERGR"
     gene            5557..5802
                     /locus_tag="VGA15_19090"
     CDS             5557..5802
                     /locus_tag="VGA15_19090"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9589836.1"
                     /translation="MTLEDGTVAGSVLTMDQAVKYAVDHLHQPLTEALRMASLYPAQF
                     LRLDRDRGRIAPRFVADMVLLGEDLSVRRVWIAGVSG"
     gene            5970..6830
                     /locus_tag="VGA15_19095"
     CDS             5970..6830
                     /locus_tag="VGA15_19095"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9589837.1"
                     /translation="MIKKLMGCPGWLKLSEDRTSFIFDPKKAEIVQKIFEASISGLGG
                     YTIAKQLNAKKVPAFGPSPKWDQSTIHNMLRNRATIGEHQPKRIRNGKRVPDGDPIPG
                     FYPAAIDESLFDAAQEARQNNLASGRGRKGKLLTNLFRGIPTCAYCASPVLFKSKSNI
                     KSLICSNVIEHQGCYRMAWSYKDFENSFLDFVKNRASDPTIEQQERESLEELVSHIRA
                     LSGSDVYDARVGIAASLKATVSELKIASAGPTPVAGKLDARIRLDRAGRYFVVRFRSG
                     LAHTGSPSGK"
     gene            complement(6917..7879)
                     /locus_tag="VGA15_19100"
     CDS             complement(6917..7879)
                     /locus_tag="VGA15_19100"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9589838.1"
                     /translation="MGRHTYPCGRCGTQGCTWFRHSHGYACARSRAAAAEFTGAFLKS
                     VHREFQEGGPTRKGTRSALHRDMLFPDIIGSFRLDQAWGLFQLSAVAHDNHVAYFGAT
                     EPTGHPDDKWGFAVQAALSIKNIPTGPGDVINLQGVYTDGATRYNFQSLFPQSFALYG
                     SSNIAYQSLGFAGLADATFSGTTAATGTGLETVKTWGFRGGFTHNWSPNWATSIYGAY
                     AQLHYGNAGKATICANAVALLGFVGTCNPNFNLGVIGGNIVWTPVQNLAFTADVNWTQ
                     LDQKYSGTISGPGTASLSVAKPAALYELKDQNFVSVLLRAQRNF"
     gene            complement(8066..9589)
                     /locus_tag="VGA15_19105"
     CDS             complement(8066..9589)
                     /locus_tag="VGA15_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9589839.1"
                     /translation="MKMVKSLILGSAAGLLAMSGAQAADLPLKAKAVEYVRICSLYGA
                     GFFYIPGTDTCIKLGGYLRADTVLNTNNDIGFAGGSTGARNRLSNYYTSRARFDMTVD
                     TRTATEYGVVRTFADMVFTWTGGGQVGAGTGLTGGATTYDSVNPGGSASGGIGGANFG
                     LYHAFIQFAGFTFGRTVSIFDAPWQSYPAGGPDTLPGGSNHVTGVNAVSYTAEFGQGV
                     SAAVALQDPTQYDQSNVFNLSGASAAGMVTGLYGTNDLGGTRAPDIIGRLGIDQAWGL
                     AQISVVAHNNHAAYYGITEDTGHPGDKWGFAVQGALSIKNIPTGPGDVVNMQAVYTDG
                     ATRYNFQSLFPQSFALYGGSGLPGAYQSIGIAGLADATFSAGTGLETVKTWGFRGGFT
                     HNWSPNWASAIYGGYGQLRYGNAGAATICNNARTLLGLVGTCSPDFNLGVIGANVVWT
                     PVKNLAFTADVNWTQLDQKYSGTIVSPTLAAVAKPGVVYQLKDQNAVNMLLRAQRNF"
BASE COUNT         1826 a         2984 c         2873 g         1908 t
ORIGIN      
        1 ggcggcctcg atgggcaggg ccagaaatcg ctgcttcttg ccttgttcgg cgtgctgcgc
       61 ggcgtcaccg ggcgcatcac ggtcgagggc cgcgaggtgc gccccggctc tccggcggcc
      121 gcgaagtcgc ccgcggtcgg catcgcgctg gtgcccgagg atcgcaagac cgaagggttg
      181 atgctgccga tgtcgatcgc cgacaatctg gcgatcgcat cgctcgatgc catctcgacc
      241 gggcctttcg tcgatcgctc caaggagcgc gccgcggtcg agcgcggcat cgcccggctg
      301 cagatcaaga tcggcgcacc gggcgatgcg gtctcgaccc tgtcgggcgg caaccagcag
      361 aaggtcgtga tcgccaaatg gctgatgacc gagccgcgca tcatcctgct taacgatccg
      421 acgcgcggca tcgatgtcgg caccaagcag gagctctacc ggctgatgcg cgagctcgcc
      481 gaccagggcg ccgccatcct gttctactcg accgattatg acgagctgat cggctgctgc
      541 gaccgggtgg ctatcatgta tgacgggcgc attgtgcgcg agctcgaagg ctccgagctc
      601 accgagacca acatcatcgc gagtgccctc aacatcgatg ccgcaacatc ggcctttgcc
      661 gcagccgggg ccgcccatgc ttgacgatct cgccatcagg ctgcgccaga atatcggttt
      721 cgtcatcgcc atcctgctcg tctgcacctc ctatctcctc tacaacctgg cacatccgaa
      781 gggcttctct tcggccgtgc tggtgcagaa tggcgacgag atcttcgcgc tcgccatgct
      841 ggccatggcc cagaccgtgc cggtgctgat gtcggggctc gatctctcgg tcggcgccgt
      901 gatgaccatg gtgggctgct tcgcgagcta tctgctggcc ggcacggcag gcggcacgcc
      961 gctgcatctc gagattttcg gcctgtatct tggcctcggc accttcccgg gcggcacctc
     1021 cggtatcctg ctcggcatcg ccgcatgcgt cgcgatcggc acgctcgccg gcttcatcaa
     1081 tggctgcgtc gtcgtctatg gacgcatcca gccgatcatc gcgacgctcg caaccggcgc
     1141 ggtctatatc ggcatcgcgc tgttcctgcg cccgaccccc ggcggcaaga tcgatgagga
     1201 tctcaactgg gcgctgacaa attcactcgg cgatttcgcc tcgacggtgc acatcttcga
     1261 cgatggcgcg gcaggctggt ttgcgccctt cgcctggatt ccggtgccct tcgtgctcct
     1321 ggtcctgatc gcgctctttg tctggatgcc gttccggcgc tctgtcctgg gccgcgcggt
     1381 ctatgcggtc ggctccgccg aaggagcagc ctatatgtcg gggcttccga tcgaccgcgc
     1441 caagatcgcg gctttcacgc tcgccggttt cttcgccggc tgcggcggcc ttttccttgc
     1501 catccagacc tcatcaggca atgccgatat tccgcaggct ggcgcctata cgctcaactc
     1561 catcgcctcg gtcgtcatcg gcggcacctc gctgctcggc ggcaccggca gtgccatcgg
     1621 ctcgatcttc ggcgccatga tcctgcgcgt catttccttc ttcttccgca tcttcgacat
     1681 cgcccccttg ctgcagccgc tgttcgaggg cgtgatcctg ctggtggcag tcagcatcgg
     1741 ggcgctgggc gtactgcgcg ttaggaacac gctggagctg ttccgatgac gacgatgagc
     1801 ctcggcctct cgcgcgacga ccgcccgatc ctgatcgcgg ccctgttcat cctcgtcatc
     1861 ctggtggcgg gcaccaccta tacgatcgcc cgtttcggca gcgctccgct attgtcgccg
     1921 acctatctgc tgcagcaact gcagatcggc tccttcctcg gcatcgtggc ggccgggatg
     1981 atgatcgtca tcctgatcgc ccagatcgat ctgtcggtgc cctggacact ggcggccgcc
     2041 gccatgatgg cgacgtcgat cggcgggccg ctcgccatcc cggtcgggct tggcatcggc
     2101 ctcctggtcg gcctcgttaa cggcatcggg gtggcctatt tgcgcgtgcc ctcgatgatc
     2161 ttcacgttag gcgtcaatgc cgtgatgcgc ggtctgatgg tcgcgcatac cggcggctat
     2221 gcgccccaga ccaccgccac tgagctgatg cggacgctcg ccgcggagcg cacgctcggc
     2281 atccccaacg ccttgttcgt ctgggccgcg gtgtcggtgc tggtggtcgt catcctgcag
     2341 cgcactccgc tcggccgcta catctacgcc atcggcaaca aagaggcggc ggcctatctg
     2401 gccggcgtca atacgcgccg cgtcaccgtc atctgcttcg tgctttgcgg tctcgcggcc
     2461 gctctcgctg gcgtgctgct cgccggttat tccaccaagg cctatcaggc gatgggcgac
     2521 gcctatctgc tgccttcgat cgccgcggtc gtgatcgggg gcacaaacat cctgggaggt
     2581 cgcggccgtt atctcggcac gttggtcggc gtggtgctga tcgtgctcct caacagcgtg
     2641 ctgtcgatca tggacatgcc cgaggccggc cgacaggtga tctacggcct cgtcatcatc
     2701 cagatgctgc tcgtctatgg acgcggcgaa cgcatcacaa gctagaaccg ccatcgagcg
     2761 tccatcaacc gagcttgcag ccgttcgccg taacgtagat cgaatagaag gacgtcgttc
     2821 cgcaaatgaa caagcggttg agcttggcgc cgccgaaagt cacgttcgcg accatttcgg
     2881 gatcaggatt ttgccgatca gcgagccgtc ggaatcatag caatggacgc cctctgcggt
     2941 gctcgtccaa atgcggccct tccgatcgac gcgaaaaccg tcgaacaagc ccgctgtgga
     3001 ctccgcgaag atctcgccgc ccgacagctt ggccccgctc gcgtcggcag tgaagtggcg
     3061 aatgtggcgc ggaccgtcct ctttgtgcgt cgcgcccgtg tcagcgacat agagatattt
     3121 ctcgtcgggc gagaaggcga tgccgttcgg acgaaccata tcggtgataa ccgcggcgac
     3181 cgcgccagat ttggcgtcga ctcgatatac atggcagccc tcgatctcct gtccggcgcg
     3241 atccccttcg taatcggtgt cgatgccgta ggcggggtcg gtgaaccaga tggagtcatc
     3301 cgatttcacc acgatgtcgt tgggcgaatt cagacgcctg ccgttccact tttccgcaat
     3361 gacggaaagc gagccgtcaa tctcggtgcg ggtcacgcgg cgcgtgagat gctcgcatgt
     3421 gaccagccgg ccttgattgt cgacggtgtt gccgttcgaa ttgttggagc gctcgcggaa
     3481 gaccgagact tcgcctgagg gttcgacgaa ccgcatcatg cgattgttcg gaatgtctga
     3541 ccagacgaga tagtggcctg ccgcgaacca agccggaccc tcagcccagc gcgctcccga
     3601 ccacaagcgc tcgacgcgca cgtgaccggc aaagcaagct ttgaagccct gatcgagaac
     3661 gacgatgtcc gttccctcga cttgaccata ccacatcgcc ccccccacct ttccatatcg
     3721 ccttgcgatt cttttcgatg cgaccgacag acgcctcttg gccaaggact tggatcgggc
     3781 gcactgtcgc cggcgttcgg ccgaacgccc gtcgcttctc ccataccgtc ggattaagca
     3841 agccgtcggc ctgatccccg ttcattctta agcgagaatt tgtccgcctg ttcgcaacgg
     3901 cgccgtgacg ctcatcgcga aacgtccgca gatttgctca tgttcttggg ctcccctttt
     3961 cgcgatgcgc agccgcccgg cccttcgaag atgcgcatgc aggcgtcctc gatatgggtg
     4021 cgcatggcga gccgcgcggc gtccttgtct cgcgcttcga tggcttcgaa catcacgacg
     4081 tgttcggtct gcacggctct caaatgttcg agaggccgtg taagcgtgag gctacgcgcg
     4141 agattgatcg cgaacaggct ggccgctctg acggccagca gatcatggtg cgtaacggct
     4201 acgcctatat cggccatgtc tgttcgaatg gtttttccgt cgtcgacgtt cgcgacccga
     4261 ccagaccgaa gacggtcaac tgcgtccaga atccgcccaa cacctggagc ctccatctgc
     4321 aggtgcacga cgacctactg cttctcgttc acggcaggga tatgttctct cagcccgaaa
     4381 tggcgggcga gcggaactac tacaaaccga agtccggcac gcacggctac gtggaagcgc
     4441 cgcgcgcgcg aaactggtcc gccggcatgg ccgtgttcga aatctccaaa ccagcggagc
     4501 cgcgtcaaat cggcttcatg cctgtcgatg gtgttgggct gcatcgcatc tggtatgttg
     4561 gcggacgctg ggcctacgcc tcggcgatga tggagggttt ctcggactac attctcatca
     4621 ccattgatat gcaggaccct gccaaaccgc agatcgtcgg ccgctattgg ctgccgggaa
     4681 tgaacctcgc cgcaggagaa acggccaact ggccgctgga gcgcgggcgc taagggctgc
     4741 atcatgcagt cgtcgccgac gatattgcct attgcgcgtg gcgcgacggc tgcctggtga
     4801 cggtcgacgt tgccgacaag agcaatccga agcaaatcgt ccataggaac cggtcgctgc
     4861 cattcggcgg cggcacgcac aactgcttgc ctctgcccga ccgcgatctt acgccagacc
     4921 aattcttttc ggtgacggtg cctagatcat tcttattttc gccccagcga acgcgagcgc
     4981 ctattctgag tcctcgggat tggtagccgg tcattgtcgc aactcaaaaa agatgacgcc
     5041 gacaataagt gtgtggctcg gtggacagtg aattaaagag ccgcgccgat ccatcatcca
     5101 ccgatgggtt aagatcggga cccgtaaaac cacgtgccat cgggcggcta gttctgtggg
     5161 ccggaacgtt cctgcatttt tggcgcttga gtacccgttg tgttgcgatg gagttgagta
     5221 tggggcgcac aggctgggat gcgaagttcc ccgaaccgat ccctctcgcg gatggcagca
     5281 agctcgcgac gttgcgcgat gctattgctc acctcggcaa gatcattcca aagccgaaca
     5341 tgacatccgg aggcaaaccg ccccagacgg gaccggccta cgcttcgaaa cattggagca
     5401 cggcggggaa tatcccgaca caatgcctca agcgatcaaa ctggtcgatg ctgagggtcg
     5461 ctgctgttcc tggtcggcga tgcgatgccg ccggccgcgt cactcggaac gacttttgcg
     5521 ctcaatggcc gcacggtaac ctagcgcgat ggcaagctga cgctcgagga cgggacggtc
     5581 gcgggctcgg tcctcaccat ggatcaggcc gtcaaatacg cggtcgacca cctccaccaa
     5641 ccactcaccg aggcgctgcg gatggcgagc ctctacccgg cccagttcct gcgcctcgac
     5701 cgggaccggg gccggatcgc cccccgcttc gtcgccgaca tggtgctgct gggtgaggac
     5761 ctgtcggtca ggagagtgtg gatcgccgga gtcagcgggt aaccttgaca ttaccatcgc
     5821 atcgcgctgg gaggtcacaa tgcgtgcgtt ccgagatcgt cgccattaag atgcagcggg
     5881 ccgagttata atgacatccg gatgaatagg ctggtcgatc cggtgccatc ttgtgaaagg
     5941 ttcgaaatac cgtggtgcgg gagcaggcaa tgatcaagaa gcttatggga tgtcctggat
     6001 ggctgaagct gtctgaggat cgaacctcct tcatcttcga tccaaaaaaa gcggagattg
     6061 ttcagaaaat ttttgaagct tcgatttcag gcctcggagg ctacacgata gctaagcaac
     6121 taaacgcgaa gaaagtgccg gcatttggtc cttctcctaa atgggatcag tcaaccattc
     6181 acaacatgct ccgcaatcgc gctacgatcg gcgagcacca gccgaagcga atccggaatg
     6241 gaaaacgcgt tccagacggc gatccgattc cgggatttta tccagcagcg atcgacgaga
     6301 gccttttcga cgcggcccaa gaagcacggc aaaataatct ggcttcgggc cgaggtcgca
     6361 aggggaagct gcttaccaat ctctttcgcg gaattccaac atgcgcctat tgcgcaagtc
     6421 cggtgctgtt caaaagcaaa tcaaacataa aaagccttat ctgctcgaac gtgatcgagc
     6481 accagggctg ttatcgaatg gcctggtcct acaaggattt tgagaattcg tttttggact
     6541 ttgttaaaaa tcgcgcgtct gatccaacaa tcgagcaaca ggagcgagaa tctctagagg
     6601 agcttgtcag tcacattcga gctctgtctg gctctgacgt ctacgatgcg cgcgtgggca
     6661 tcgctgcatc tctcaaggct accgtatccg aactaaagat cgcatccgcg ggaccgacgc
     6721 ctgtcgcagg caagctagat gcgcggatcc gcctcgatcg cgcgggacgt tatttcgtgg
     6781 tccggtttcg gagtggtctg gctcacactg gttctccaag cggcaagtag gtgctggatc
     6841 tgagcgcgcg cacaaagcaa aacccccggc agcttcctgc cggggggtct gtaaggccgt
     6901 gatcggacga actggattag aagttgcgct gagcgcggag cagcacgctg acgaagttct
     6961 ggtccttgag ctcatacaaa gctgccggct tggcaaccga cagagacgcc gtgcccgggc
     7021 ccgaaatcgt acccgagtac ttctggtcca attgcgtcca gttgacgtcg gcggtgaagg
     7081 ccaggttctg gaccggggtc cagacgatgt tgccaccgat gactcccaag ttgaagttag
     7141 ggttgcaggt gccgacgaag ccaagcaacg ccaccgcgtt ggcacagatc gtcgccttgc
     7201 cagcattacc gtaatgtaac tgggcataag caccataaat gctggtggcc cagttagggg
     7261 accagttgtg ggtgaaacca ccgcggaagc cccaagtctt gaccgtctca agcccggtgc
     7321 ccgtggccgc cgtagtccca ctgaaggtag catcagctag accggcaaag cccaagctct
     7381 ggtacgcgat attgctgctg ccatacaacg cgaatgattg cgggaacaag ctctggaagt
     7441 tatagcgggt tgcaccatcc gtatagacgc cctgcaggtt gatgacgtca cctggcccag
     7501 tcgggatgtt cttgatcgac aacgccgcct ggactgcaaa gccccacttg tcatccgggt
     7561 gaccagttgg ttcggtcgca ccgaagtagg caacgtggtt gtcgtgcgca acagcggaca
     7621 actggaatag accccaggct tggtcaaggc ggaacgaacc gatgatgtcc ggaaagagca
     7681 tatcacggtg caatgcagaa cgggtcccct ttcgcgtggg tcccccttct tgaaattccc
     7741 gatgcaccga cttcaaaaaa gccccagtaa actccgccgc tgctgctcgg ctacgagcac
     7801 acgcgtatcc atgggaatga cggaaccaag tgcaaccctg tgtcccgcat cggccacatg
     7861 gatacgtatg gcgacccatg gactcgtgtt gcggagctag ttactcgagg gggcgcatgg
     7921 acacaccgga gccagaggct ctcagcagcc aacgaaggtc gcgtccgccc aattcccctt
     7981 acttacaata aagactctac ccatgcgcaa aggaaagccc ccagcggttt cccgccgggg
     8041 gctctgttag gtcgttagac cgtgcttaga agttgcgctg agcgcgcagc agcatgttga
     8101 cggcgttctg gtccttgagc tgatacacaa ctcccggctt ggcaaccgcc gcaagcgtcg
     8161 gggacacaat cgtgcccgag tacttctggt ccaactgcgt ccagttgacg tccgcggtga
     8221 acgccaggtt cttgaccggg gtccagacga cgttggcacc gatgactccc aagttaaagt
     8281 cggggctgca ggtgccgacg agaccaagca acgtccgcgc gttgttacag atcgtcgccg
     8341 cgccagcatt accgtaacgc agctggccat agccaccgta gatggcggag gcccagttag
     8401 gggaccagtt gtgagtgaaa ccaccccgga agccccaggt cttgaccgtc tcaagcccgg
     8461 tgccggcact gaacgtagca tcagctagac cggcaatccc gatgctctgg tacgcgccag
     8521 gcaagccact gccgccatac aacgcgaaag attgcgggaa caagctctgg aagttatagc
     8581 gggttgcacc gtccgtatag acggcctgca tgttgacgac gtcacccggc ccagtcggga
     8641 tgttcttgat cgacagggca ccctggactg caaagcccca cttgtcaccc gggtgaccgg
     8701 tatcctcggt tataccatag taggcagcgt ggttgttgtg cgcaacaacg gaaatctggg
     8761 ctagacccca ggcttggtca atgcccaacc tcccgataat gtccggagca cgcgtaccgc
     8821 caaggtcatt ggtgccgtag agaccagtga ccatgcccgc ggcgctggcg cccgacaagt
     8881 taaagacgtt cgactggtca tattgtgtcg gatcctgcaa cgcgaccgca gccgagacgc
     8941 cttggccgaa ctccgcggta taggagaccg cgttcacgcc ggtgacgtgg ttgctgccac
     9001 cgggaagggt gtcagggccg ccggccggat agctctgcca cggcgcatcg aagatcgaga
     9061 ccgtccgacc aaacgtgaac ccagcgaact ggatgaacgc gtggtagagg ccgaagttgg
     9121 cgccgccaat cccgccagaa gcgcttccgc ccggattgac cgagtcatac gtagtagcac
     9181 caccggtgag gccggtgccg gcgccgactt ggccgccgcc cgtccaggtg aagaccatat
     9241 cggcgaaagt gcggaccacg ccgtactcgg tcgcggtgcg cgtatcgacg gtcatgtcaa
     9301 agcgggcgcg agacgtgtag tagttgctca ggcggttgcg cgcaccggtg gagccccccg
     9361 cgaagccaat gtcgttgttg gtgttgagta ccgtgtcggc gcgcagataa ccacccagct
     9421 tgatgcaggt gtcggtgccc ggaatgtaga agaaaccggc accatacagg gagcagatcc
     9481 tcacatattc gaccgctttg gccttcaagg ggagatcggc cgcctgtgcc ccactcatag
     9541 cgagcaaacc cgccgctgag ccgaggataa ggctcttaac catcttcatg t
//
LOCUS       DASWGM010000680         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2024756,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000680 DASWGM010000000
VERSION     DASWGM010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024756"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1624
                     /locus_tag="VGA15_19110"
     CDS             <1..>1624
                     /locus_tag="VGA15_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX9589840.1"
                     /translation="AGEYFAEEVRRDVFERYGEKKLYEGGLSVRTTLDPKLQVMARKT
                     MAAGLVTYDEAQGWRGATSKLDISGDWGVKLADVKSLSDISPWRMAVVLETSDQSARI
                     GFQPGRELGGAVAKERQTGNITLDGVRWAKAASGPARGKTPSSVSQVLSPGDIVYADP
                     LIAKDGSIVEGQFRLRQLPEVSGAMVVMDPWTGRVLAMVGGFSFDQSQFNRATQAYRQ
                     PGSSFKPIVYSSALDNGYTPSTVVVDAPIEIDQGQGGGVWRPENYSAGHYRGPTTLRN
                     ALRFSLNTVTVRLAQDIGMPLIGEYAKRFGVYDELPNYLSYALGAGETTVMRMVTAYS
                     MFANGGRRVKATLIDRIQDRYGHTIFKHDARECRGCDAPGGWKNQPEPQLVDRREQVL
                     DAMTAYQITSMMEGVVQGGTATVMREVGKPIAGKTGTTNDEKDVWFIGFSPDLVVGLY
                     LGYDKPRSLGRSAQGGRTAAPIAKDFMKLALADKPAIPFKVPAGIKLITVDQMSGMRA
                     GPGSGRTVLEAFKPGTAPPDNYSVIGVADADGR"
BASE COUNT          315 a          482 c          537 g          290 t
ORIGIN      
        1 cgccggcgaa tatttcgccg aggaagtccg ccgcgatgtg ttcgagcggt acggcgaaaa
       61 gaaactctat gaaggcggcc tgtcggtacg gaccacgctg gatccgaagc tgcaggtgat
      121 ggcgcgcaag accatggcgg cgggcctcgt cacctatgac gaagcgcagg gctggcgcgg
      181 tgctaccagc aagctcgata tctcgggcga ttggggcgtg aagcttgccg acgtcaaatc
      241 gctctcggac atctcgccct ggcggatggc cgtggtgctg gagaccagcg accagtccgc
      301 ccggattggt tttcagcccg gtcgcgagct gggtggcgcc gtcgccaagg aacggcaaac
      361 cggaaacatt acgcttgacg gcgtaagatg ggcaaaggcc gcctcgggcc cggcacgcgg
      421 caagacgccg tcatcggttt cgcaggttct aagccccggc gacattgtct atgccgaccc
      481 gctgattgca aaagatggca gtatagtcga gggccagttc cggctgcgtc agctcccgga
      541 ggtttccggc gccatggtgg tgatggaccc ctggaccggc cgcgtgctgg cgatggtcgg
      601 cggtttctcg ttcgaccaga gccagttcaa ccgcgcgacg caggcctacc ggcagccggg
      661 atcctccttc aaacccatcg tgtactcgtc cgcgctggac aacggctata cgccatcgac
      721 ggtcgtggtc gatgcgccga tcgaaatcga tcaggggcaa ggcgggggcg tctggcgtcc
      781 ggagaactat tcggccggcc attatcgcgg ccccactacg ttgcgtaacg cgctgagatt
      841 ttcgcttaac acagtgacgg tgcggttggc gcaggacatc ggcatgccct tgatcggcga
      901 atatgccaag cgcttcgggg tctatgacga gttgccgaat tacctgtcct acgcactggg
      961 cgccggcgag accacggtga tgcgaatggt cacggcctat tcgatgttcg ccaatggcgg
     1021 ccggcgggtg aaggctaccc tgatcgatcg catccaggac cgctacggcc ataccatctt
     1081 caagcatgac gcacgcgaat gccgcggctg cgacgcgccg ggtggctgga agaatcaacc
     1141 cgaaccccag ctggtagatc gcagggaaca ggtgctggat gcgatgacgg cctatcagat
     1201 tacttcgatg atggaaggcg ttgtccaggg cggcacggcg acggtgatgc gcgaagtcgg
     1261 caagccgatc gcaggcaaaa ccggcaccac caatgatgaa aaagatgtct ggttcatcgg
     1321 cttttcgccg gatctcgtgg tcggactcta tcttggctac gacaagcccc gcagcctggg
     1381 cagaagcgca caaggtggcc gcaccgccgc gccgatcgcc aaggatttta tgaaactcgc
     1441 gctcgccgac aagccggcga ttccgttcaa ggtgcctgcg ggtatcaagc tgatcaccgt
     1501 cgatcagatg tcgggaatgc gggccggtcc gggcagtgga aggacggttc tcgaagcctt
     1561 caagccgggc accgcgccgc cggataatta ctccgtgatc ggcgttgccg atgccgacgg
     1621 gcgg
//
LOCUS       DASWGM010000681         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2024928,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000681 DASWGM010000000
VERSION     DASWGM010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2024928"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VGA15_19115"
     CDS             complement(<1..187)
                     /locus_tag="VGA15_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9589841.1"
                     /translation="MQLEVDIASKAFTNAAGERFDVLADLNFALGAGEVGVFVGPSGC
                     GKSTMLRILAGLDHDYQG"
     gene            complement(177..914)
                     /locus_tag="VGA15_19120"
     CDS             complement(177..914)
                     /locus_tag="VGA15_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9589842.1"
                     /translation="MLRLLSFAVFLAVWWGAALLAGAGKLPPPPAVLAAAVAEASSGA
                     LFFNLGVTLARVALAFTLAMTLGAAIGYLMGRVRLANRLGDPWLILLLNLPALVVIVL
                     AYIWAGLTEVAAIAAIAINKLPTAVVTVREGARALDAALDEMATVFAIPRWRAFRHVI
                     LPQLAPYIAAAARSGLSLVWKIVLVAELLGRPNGVGFEIGVAFQLFDIPLLLAYALSF
                     VAVVLAIETMLVQPFEVRVSRWRPRAA"
     gene            complement(916..1896)
                     /locus_tag="VGA15_19125"
     CDS             complement(916..1896)
                     /locus_tag="VGA15_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589843.1"
                     /translation="MKLFTRILLATAVFTALHIVPSRAAETIRLAVQKTGTFAWELAV
                     IRAHGLDRQANLTIEVSELASPEAGKVALRGGAADVIVSDWLWVSRERGLGAKLTFYP
                     YSSALGAVMVPDSSSIRTLADLKGRKLAVAGGPIDKSWLLLRASMKQDGIDLKSESTI
                     LYGAPPLLAAKTLSGEMDATVNYWNFCAALEAKGLRRLIGIEDLLPRLGATGRTSMIG
                     YVFDEGWGGANRDTVASFIAVTRAAKEILATSDAEWEKIAPLTGAPDTATLRAYRDRY
                     REGIPRRPIAAEEADARVLYRVLAVIGGRELVGPAPELEPGTFYRAISGD"
     gene            2022..2381
                     /locus_tag="VGA15_19130"
     CDS             2022..2381
                     /locus_tag="VGA15_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589844.1"
                     /translation="MTFAGSMRRNVCALIALVATAAVARADEVNDYPTAARSEYVFGC
                     LKANGETRQAIEQCSCSIDVIASLLPYDRYVTAETVLSMAQVRGNLGTQFRSSEQATS
                     ALNDLRRAQAEAEVRCF"
     gene            complement(2437..3219)
                     /locus_tag="VGA15_19135"
     CDS             complement(2437..3219)
                     /locus_tag="VGA15_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HEX9589845.1"
                     /translation="MQFGVRLAPAATSEPYDPWPGLVQDIFNNRAMNDGAGLIAIEMP
                     YRAEDAAIVPVTLRATLPLGDTRRVLAITLVIDQNPAPMAARFELGPDSSVSEISTRV
                     RVNNYTDVHAVAELSDGQLYMTKTYVKASGGCSAPAAKNADEAKNKLGQLRFRQFAKP
                     GEGPTSGAREAQIMVGHPNNSGLQMDQLTQLYIPPFFVNELRVWQDDSLVLAMEGGIS
                     ISEDPNIRFTYVPNGAKRFRVEAKDTDGHVFQNEWKVDSSGI"
     gene            complement(3321..>3731)
                     /locus_tag="VGA15_19140"
     CDS             complement(3321..>3731)
                     /locus_tag="VGA15_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9589846.1"
                     /translation="SLMLRAWPTAHSDNDLTVLDEQTGTLFAGDLVFLAHLPVLDGSI
                     RGWLKAIDELGAVAAHLVVPGHGQVREWAGALVDERRYLEKLAADVRGLIAGGKPITV
                     AADSAAASERSQWQLFDDYNARNATAAFSEIEWE"
BASE COUNT          727 a         1174 c         1169 g          661 t
ORIGIN      
        1 ggccctgata gtcatgatcg agaccggcga ggatcctcag catggtgctc ttgccgcagc
       61 cggatgggcc aacgaacacg ccaacttctc cggcgccgag cgcaaagttg agatcggcga
      121 gcacgtcgaa ccgctcgccg gctgcgtttg taaacgcctt gcttgcgata tcgacctcaa
      181 gctgcacggg ggcgccaccg agatacccgg acttcaaacg gctgcaccag catggtttcg
      241 atggcgagca cgaccgccac gaaacttagc gcataggcca gcaggagagg gatgtcgaag
      301 agttggaagg ccacaccgat ctcgaagccg acaccgtttg gacgtcccag caattcggca
      361 accaggacga tcttccagac cagggaaagt ccggatcgcg cggcggccgc gatatagggc
      421 gccagttgcg gcaatatcac gtgccggaac gctctccagc gcgggatcgc aaacaccgtc
      481 gccatttcgt ccagcgccgc gtcgagcgcc cgggctcctt cgcgcaccgt gacgaccgca
      541 gttggcagct tgttgatggc gatggccgca atcgcggcga cttccgtcaa tccggcccag
      601 atatacgcca gcacgatgac gacgagcgcc ggcaggttaa gcagcaggat cagccaggga
      661 tcgccgagcc ggttggcaag tcgtacccga cccatcagat agccgatggc ggcacccagc
      721 gtcatcgcca gcgtgaaggc gagggcaact cgggccaacg ttacgccgag attgaagaac
      781 agcgcgccgg aactggcttc cgcgacagct gccgccagca cggcgggagg aggcggcagc
      841 tttcccgcgc cggcaagcag cgcggcgccc caccagaccg caagaaacac ggcgaacgac
      901 aggagacgga gcatctcagt ctccggagat ggcgcgatag aatgtgccgg gttcaagttc
      961 tggcgccggg ccgacaagct cgcgaccgcc gatcaccgcc agcacgcgat aaagaacgcg
     1021 cgcgtcggct tcttcggcgg cgatcgggcg gcggggaatt ccttcccgat aacggtcgcg
     1081 ataggcgcgc aaggttgcgg tgtcgggggc acccgtcagc ggcgcgatct tttcccactc
     1141 cgcatccgag gtcgccaaaa tctccttcgc cgcgcgggtc accgcgataa agctggcaac
     1201 ggtgtcgcgg ttcgcgccgc cccatccttc gtcgaagacg tagccgatca tcgacgtgcg
     1261 gcccgtggcg ccgagcctcg gcagaagatc ctcgatccca atgagacggc ggaggccttt
     1321 ggcttccagc gcggcgcaga aattccaata attgacggtg gcatccattt cgccgctgag
     1381 cgtcttcgcc gccagcagcg ggggcgcacc gtacaggatc gtggactccg acttcaggtc
     1441 gatgccatcc tgcttcatcg atgcccgcaa cagcagccag cttttgtcga tcgggccgcc
     1501 ggcgacggcg agcttgcggc ctttgaggtc cgccaaggtc cggatcgaag atgaatccgg
     1561 taccatcacc gcgccaagcg cgcttgaata cggatagaac gtcaatttgg cgccgagccc
     1621 gcgctcgcgc gacacccaca gccagtcgga gacgatcaca tcggcggcgc cgccgcggag
     1681 cgcgaccttg cctgcctcgg gactcgcaag ttcgctcacc tcaatcgtga ggttggcctg
     1741 cctgtcgagg ccgtgagccc ggatcacggc aagttcccag gcgaatgttc cggtcttttg
     1801 cactgcaagt cgtatcgttt ccgcagcgcg ggatggaacg atatgcagcg cggtgaaaac
     1861 ggccgtagcc agcagaatac gggtgaagag tttcattgtg cccttgttat cggagcccga
     1921 tgcccgacag gcatctcagg ttcgacaaga tgtgcgatca gtcgtttcct cgacaggctc
     1981 gttttccata tgatagcaca gctcccagca ggaggaatgc gatgacattt gccggatcga
     2041 tgcgacgtaa tgtctgcgcc ctgatcgcgc tggtagcaac ggccgctgtc gcacgtgccg
     2101 acgaagtcaa tgactatccc acggcggcga gatcggagta tgtgtttggc tgcctgaagg
     2161 cgaatgggga aacgcggcag gcgatcgagc aatgctcctg ctcgatcgac gtcatcgcgt
     2221 cgctgctgcc ctacgatcgc tacgtcaccg ccgagaccgt gctgagcatg gcccaggtac
     2281 ggggaaatct cggtacgcaa ttccgctcgt ccgagcaggc gacaagcgcg ctcaacgatc
     2341 tcaggcgtgc ccaggccgaa gcggaagtga gatgtttcta agccgcgcgg tgctgcgcgg
     2401 ccgtatcgtg ccaatccggg cggcatcgca ccgcccttaa atcccggaac tgtcgacctt
     2461 ccattcgttc tggaacacgt ggccgtcggt gtccttggcc tcgacccgga agcgcttcgc
     2521 gccgttcgga acataggtaa atcggatatt gggatcttcg gaaattgaaa ttccgccttc
     2581 catcgccaaa actaggctgt cgtcctgcca cacccgcaat tcattgacga agaacggcgg
     2641 aatatagagt tgggtaagct ggtccatctg caggccggaa ttgttcgggt ggccgaccat
     2701 gatctgggct tcccgggcgc cgctggtcgg gccctcgccg ggctttgcga actgtctgaa
     2761 acgcagctgg ccgagcttgt ttttcgcctc gtctgcgttc ttggcggcgg gggccgagca
     2821 tccgccggac gccttcacgt aggttttcgt catgtagagc tggccgtcgc tcagctccgc
     2881 gacggcatga acgtcggtgt aattgttgac gcgcacgcgc gttgagattt ccgataccga
     2941 tgagtcgggt ccgagttcga accgcgccgc catcggcgcc gggttctggt cgatcaccag
     3001 cgtgatcgcc agcacgcgcc tggtgtcacc gagtggcagc gtcgcgcgca gggttacggg
     3061 aacgatcgcc gcgtcctcgg cgcggtacgg catctcgatg gcgatcaacc cggcgccgtc
     3121 gttcatcgcg cgattgttga agatgtcctg gaccaggccg ggccacggat catagggctc
     3181 cgacgtcgca gcaggcgcga gacggacgcc gaactgcatc gcagccaaaa gaccggcgat
     3241 gcagagcagg cgggaatggt gtcccaacat gatcgcgcct cctccgaaag caacgaaccc
     3301 aatgtacttc attgagccgg ctattcccat tctatttccg aaaatgcggc cgttgcgtta
     3361 cgcgcattgt aatcgtcaaa tagttgccat tgcgaccgtt cggaggcagc ggcgctatcg
     3421 gccgcgaccg ttatcggctt gccaccggcg atcagcccgc ggacatccgc ggcgagtttt
     3481 tccagatagc gacgttcgtc gaccagcgca cctgcccact cgcgcacctg tccatgaccg
     3541 ggaaccacga ggtgggcagc aacagcgcca agctcatcga tggccttcag ccagccgcga
     3601 atgctgccgt cgagcacggg caggtgagca aggaacacca gatcgcccgc gaacaaggta
     3661 ccggtttgct cgtcgagcac ggtgaggtca ttgtcgctgt gcgcggtcgg ccacgctcgc
     3721 agcatgagac t
//
LOCUS       DASWGM010000682         5530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2025864,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000682 DASWGM010000000
VERSION     DASWGM010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025864"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VGA15_19145"
     CDS             <1..696
                     /locus_tag="VGA15_19145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9589847.1"
                     /translation="PARVLETAAGTGIVTRKLRDALPAATRLTATDLNPPMLDIARTK
                     FRSGEQVEFQPADAMALPFADGSFDAVVCQFGVMFFPDKAKSCSEVLRVLAPGGRYVF
                     SVWDSHRYNPFGRLAHQVAGSFFPSDPPQFYRVPFSCHQIDPIKELLIEAGFTGIDAA
                     VVSLDKEVPDAASFARAAIYGNPLIDQIRTRGGVEPELIVSALAQEYRREFGADPGRM
                     PLQAIVFSATKRS"
     gene            802..996
                     /locus_tag="VGA15_19150"
     CDS             802..996
                     /locus_tag="VGA15_19150"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX9589848.1"
                     /translation="MPVAKVYVPEGILTPDQRREIVKGIHEVIVGVEKRPADSPTYVL
                     INEVPAGGWGATGNVYVPRT"
     gene            1182..1466
                     /locus_tag="VGA15_19155"
     CDS             1182..1466
                     /locus_tag="VGA15_19155"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX9589849.1"
                     /translation="MTAAVAASAAAGVSTALADTAPAANFGAPLVELHFPANLLTLEQ
                     KAAMIKGVTDVVLKTTKQPPDPARHSFVQIFETAEGGFGVDGQVFVPRAK"
     gene            1567..1902
                     /locus_tag="VGA15_19160"
     CDS             1567..1902
                     /locus_tag="VGA15_19160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX9589850.1"
                     /translation="MLKGHLDMIVLAALSAAGPAHGYAIIEEIKRRSGGAFDLPEGTI
                     YPALHRLEQAGLLKSSWMTADSGRRRRVYTLTRTGNRALVERRAVWKQFSDAIGGLLG
                     GTRPREDPA"
     gene            1899..2591
                     /locus_tag="VGA15_19165"
     CDS             1899..2591
                     /locus_tag="VGA15_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589851.1"
                     /translation="MIQDYLELLERELNFDRSLSRCVRQEVEDHLWEAVAVDLTGDML
                     VAQRRAIANFGDARAIAAQFAVVSLARHSRRAGVAAVLVIASVFIAMKARIAWYAAAQ
                     WAISDDMRAVGGLVVQVDRYAFWMSLIVGLGACVYIVSRQIPATFHPAYRRQLRRFFV
                     LCCAATAALVVSVISDGVLTALQLRGAELSAGFLVSIASMAIEIACVGILVVHIRGIT
                     LRAASTASLLST"
     gene            complement(2605..2928)
                     /locus_tag="VGA15_19170"
     CDS             complement(2605..2928)
                     /locus_tag="VGA15_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9589852.1"
                     /translation="MDALEGPGSWKRIPAATKQELRDNAYTLLGQTRDNRPPFSKEDA
                     EAIQTPTLFIGGANSKGALPVVLHTPAAHVKGSKTAMIPGTTHPMFEQAPQRFCEIVL
                     EFLAT"
     gene            3306..3848
                     /locus_tag="VGA15_19175"
     CDS             3306..3848
                     /locus_tag="VGA15_19175"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX9589853.1"
                     /translation="MTDQHFAIFDTAIGRCGIAWGARGINAVQLPMGNEEKTRARVRQ
                     RHGDIAEAPPPAEVQSAIDGIVELLAGKPNDLADIVLDLDGVPEFNRGVYDIARTIPP
                     GKTLTYGDIAKRLGGVELSRDVGQALGRNPCPIVVPCHRVLAAGGKPGGFSANGGVVT
                     KLKMLAIEGAVVNHTPSLFD"
     gene            complement(4019..4897)
                     /locus_tag="VGA15_19180"
     CDS             complement(4019..4897)
                     /locus_tag="VGA15_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9589854.1"
                     /translation="MSLKSAVGDLTIHRLIEQETTFLPALDMLPGLTPELLAENKPWM
                     QKAGAIDADDTLILCFQSYIVKTPHHTILVDSCIGNDKPRPLRPKWNMKTDDTYMHAL
                     ASSGFSVGDIDFVMCTHLHVDHVGWNTRLENGRWVPTFPNARYVFGKGEFDYWTAQHA
                     NTPVPPFGDSVLPIVEVGRAEIVRDDYAIGDHTRILPTPGHTPGHVAFTFGRGKDEAV
                     FSGDLMHSPLQARYPELSAKFDVDQALAATTRRNFLERYCDTDTLCCTAHFPSPSTGK
                     IRRWGNGFSCEAVMAG"
     gene            complement(4915..5529)
                     /locus_tag="VGA15_19185"
     CDS             complement(4915..5529)
                     /locus_tag="VGA15_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9589855.1"
                     /translation="MKLTFSPASPFARKVRIAAIELGLIDKIEFVPTTVAPGQPNDAY
                     SQITPLKKLPALILDNGDVIVDSYVIVEYLDELAGGGKLIPASGPTRWKVKSDHSLLQ
                     GMLDAMLLCRYEKMVRPQGLQWQAWSDDHWNRAWSGMARFEKQADVLSGPFDIVQIGL
                     VCVLGYADFRFADCGWRKAYPKLDAFHEKMLARPSVKISVPPAA"
BASE COUNT          993 a         1706 c         1761 g         1070 t
ORIGIN      
        1 ccggcgcggg tgctggagac ggcggccggc accgggatcg tcacgcgaaa gctgcgcgat
       61 gcattgcctg ccgcgacacg cctgacggcg accgatctca atccgccgat gctcgacatc
      121 gcgcgcacca agtttcgatc aggcgagcag gtcgagttcc agcccgccga tgccatggcg
      181 cttcccttcg cggatggaag ttttgatgcc gtcgtgtgcc agttcggcgt gatgttcttt
      241 ccggacaaag ccaaatcctg ttccgaggtt ttgcgtgtgc tagcccccgg cggccgttat
      301 gtgttcagtg tctgggattc gcatcgctac aatccattcg gccgtctggc acatcaggtt
      361 gcgggaagtt ttttcccctc cgatccacct cagttctacc gcgtgccgtt ctcctgccac
      421 cagatcgatc cgatcaagga actcctgatc gaagcgggct tcaccggcat cgacgctgcc
      481 gtcgtcagcc tggacaaaga ggtccctgac gccgcgagtt tcgcgcgcgc ggcgatctat
      541 ggtaacccgc tgatcgatca gatccggacc cggggtggcg tcgagcctga acttatcgtc
      601 agcgcgctgg cgcaagaata tcgccgtgaa ttcggcgccg atcccggacg catgccgctg
      661 caggcgatcg tgttttctgc aacaaaaaga tcgtgacggc gctcacgcat tgagaaccgc
      721 aagcggtcat cgcgttgacc ttgacaccta gtattacgag gcataccata acgaggtatc
      781 aagacaagaa aggagcctgc catgccggtt gccaaagtct atgtgccaga aggcattctc
      841 acgccggatc agcggcgcga gatcgtcaag gggattcacg aggtcatcgt cggcgtcgaa
      901 aagcggccag cggactcgcc gacctacgtg ctgatcaacg aggttccggc gggcggttgg
      961 ggcgccaccg gcaacgtcta tgtgccccgg acctgacgac gggtaactac cgccagctgc
     1021 atgcggcatt tcgccaatgg cgcggcgagc cggatgcggg ccgggctcga ttcaatgacc
     1081 catcatcacc catgcgaggt tggactatgg acatgatcaa cgagtcaaac aacgacgcgc
     1141 gaccggcaat cagcggcgga ttaaaccgca gggcggtcct gatgacggcg gccgttgccg
     1201 cgagcgcagc ggccggcgtc tcgacggcgc tggcggacac tgctccggcg gccaacttcg
     1261 gcgcaccgtt ggtggagctt cattttcccg ccaatctgct gacgctggag cagaaggccg
     1321 ccatgatcaa gggcgttacc gacgtcgttc tcaagacgac gaagcagccg ccggatccgg
     1381 cacggcattc gtttgtgcag atctttgaga cggctgaggg cggcttcggc gtcgacggtc
     1441 aggtgttcgt accgcgcgcc aagtagcgcg ccggctctgc cggcgaacca cggtgctcgc
     1501 tcttgcccgc ccgccgcttg accgctccgg cgccaaagcc tagtaatgca aggtatggaa
     1561 ggcgaaatgc tcaaggggca cctcgacatg atcgtgctcg ccgcgctgtc ggcggcgggc
     1621 ccggcgcatg gctatgcgat catcgaagaa atcaaacggc ggagcggtgg cgcgttcgat
     1681 ctacccgagg gaacgatcta cccggcactg catcgtcttg agcaggccgg cctgctcaag
     1741 agcagctgga tgaccgccga ctccgggcgg cggcggcggg tctacacact gaccaggacc
     1801 ggcaatcgcg cgctggtcga gcggcgcgcg gtctggaagc aattttccga tgccatcggc
     1861 ggcttgcttg gaggaacccg gccacgggag gacccggcat gatccaggac tatctcgaat
     1921 tgctggagcg cgagctcaat ttcgatcgat cgttgtcacg ttgcgtgcgg caggaagtcg
     1981 aagaccattt gtgggaagcc gtcgcggttg atttgacggg ggatatgctt gtcgcgcagc
     2041 ggcgcgccat cgcgaatttc ggcgatgcac gcgccatcgc cgcccagttc gcagtggtgt
     2101 cgctcgccag gcacagccga agagccggtg tcgctgcggt cttggtgatc gccagcgtct
     2161 tcattgccat gaaggcccgc atcgcatggt atgccgcggc gcagtgggcc atcagcgacg
     2221 acatgcgcgc ggtcggtggc ttggtggtac aggtcgaccg ctacgccttc tggatgtcgc
     2281 tcatcgtcgg gctcggcgcc tgcgtctaca tcgtcagccg gcagatccct gccacgttcc
     2341 atccggccta tcgccggcag ctccgccgct tctttgtgct gtgctgtgcc gcgaccgcag
     2401 cccttgtcgt ttcggtgatc agcgatggcg tgctcaccgc actccagttg cgtggagcag
     2461 aattatcggc gggatttttg gtctcgatcg catcgatggc gatcgaaatc gcatgcgtcg
     2521 ggattttggt cgttcacatt cgcggcatca cgctgcgggc ggcatccacg gcgagcttgc
     2581 tgagcacgtg atgtccgtgg gttttcaagt cgccagaaac tccagcacga tctcgcaaaa
     2641 tctctgcggc gcctgttcga acatcggatg cgtggtgccg ggaatcatcg cagtcttcga
     2701 ccccttcaca tgcgccgccg gcgtgtgcag caccacaggc aacgcgccct tgctattggc
     2761 gccgccgatg aacagtgtcg gcgtctggat cgcctccgcg tcttccttgg aaaacggcgg
     2821 ccggttgtca cgggtctgtc ccagcaaggt ataggcgttg tcgcgcagtt cctgcttcgt
     2881 cgccgccgga atgcgcttcc agctgccggg gccttcgagc gcatccatgt agacttccag
     2941 tccaccgtca acatcgcccc gggcgatgac ttcggccgcc gccgcgatcc gcgctgccag
     3001 cggggagggg ccgggcctga atgccggatc ggggcaacgt gctcctgcgc tcggacgtca
     3061 tacgcgggct tgacccgcgt atccatcaat ctttggcgag aagcgttctt gcaaagaaga
     3121 tgggatcacc gggctgcccg gtgatgacaa atcatcggcc tgttatgccg tcttttcatg
     3181 gcacggcgtc aggttccccc ggaattcggg tggatcgcaa caatctccaa gcccatattc
     3241 cagccaggcg ttaagctcaa accattgagt gccaagattt ggagccacag gatctggagc
     3301 cagccgtgac cgaccaacat tttgcaatat ttgacaccgc gatcggccgc tgcggcatcg
     3361 cgtggggcgc gcgcggcatc aatgcggtgc agctgccgat ggggaacgag gagaagaccc
     3421 gcgcccgcgt tcgccagcgc catggcgaca tcgcggaggc gccgccgcct gccgaggttc
     3481 aaagcgcgat cgacggcatc gtggaattgc tcgcgggcaa gccgaatgac ctcgccgata
     3541 tcgtgctcga tctcgacggc gttcccgagt tcaaccgcgg cgtctacgac atcgcgcgca
     3601 ccatcccgcc gggcaagacg ctcacttacg gcgatatcgc caaacggctc ggcggcgtcg
     3661 agctgtcacg cgatgtcggt caggcgctcg ggcgcaatcc ctgtccgatc gtggtgccct
     3721 gtcatcgcgt gctcgccgcc ggcggcaagc ccggcggatt ttccgccaat ggcggcgtcg
     3781 tgaccaagct gaagatgctg gcgatcgaag gcgcggtggt caatcacacg ccgagcctgt
     3841 tcgattgaac tttccgtcat tccgggatgc gccttcggtc ggcaattgcc gacctgaggc
     3901 gacaggcccg gaatccatac tcccggtcgt ggttatggat tccgggctcg ctcgtttcac
     3961 tcgcgccccg gaatgacggt gatcgcggtc agcgcggatc ggctaagcgg aaagcgcctt
     4021 aacccgccat caccgcctcg caggaaaatc cgttgcccca gcggcggatt tttcccgtcg
     4081 aaggcgaggg aaaatgcgcg gtgcagcaca gcgtgtcggt gtcgcaatag cgttccagga
     4141 aattgcgccg cgtcgtagcg gcgagagcct gatcgacatc gaatttcgcc gacaattcgg
     4201 gatagcgcgc ctgcagcggc gagtgcatca ggtcgccgct gaagaccgcc tcgtccttgc
     4261 cgcggccgaa ggtgaacgcg acatgtcccg gtgtatgccc tggcgtcggc aggatgcgcg
     4321 tatggtcgcc gatcgcataa tcgtcgcgca cgatctcggc cctgcccact tcgacgatcg
     4381 gcagcacgct gtcgccgaac ggcggtaccg gcgtgttcgc gtgttgtgcg gtccagtaat
     4441 cgaactcgcc cttgccgaac acatagcgcg cgttcgggaa cgtcggcacc cagcggccgt
     4501 tttccagtcg tgtattccag ccgacgtgat cgacgtgcaa atgcgtgcac atcacgaaat
     4561 caatgtcgcc gacggaaaag cccgaggacg cgagcgcgtg catataggtg tcgtcggtct
     4621 tcatgttcca tttcgggcgc agcgggcgcg gcttgtcgtt gccgatgcag ctgtcgacca
     4681 gaatggtgtg atgcggcgtc ttgacgatgt aggactggaa acacaggatc agggtatcgt
     4741 cggcgtcgat cgcgccggcc ttttgcatcc atggcttgtt ctcggccagc aactccggcg
     4801 tcaggcccgg cagcatgtcc agtgccggca ggaacgtggt ttcctgctcg atgaggcgat
     4861 gaatggtgag atcgccgacg gcggatttga gagacatggc aaacttcctg attcctaggc
     4921 cgccggcggc accgagattt tcaccgacgg ccgcgccagc atcttttcat gaaaggcatc
     4981 gagcttggga taggccttgc gccagccgca atcggcaaag cggaagtcgg catagccgag
     5041 cacgcaaaca aggccgatct gcacgatgtc gaacgggccc gacagcacgt cggcctgctt
     5101 ctcgaagcgc gccatgccgc tccaggcgcg gttccagtga tcgtcggacc acgcctgcca
     5161 ttgcagccct tgcggccgga ccattttttc gtagcggcac agcagcatcg catcgagcat
     5221 gccttgcagc agcgaatgat cgctcttgac cttccagcgt gtcgggccgg acgccgggat
     5281 cagttttccg ccgccggcga gttcatcgag atattcgacg atgacgtagg aatcgacgat
     5341 gacatcgcca ttgtcgagga tcagcgccgg caattttttc aagggcgtga tttgcgagta
     5401 cgcgtcattg ggctggcccg gtgcgaccgt ggtcgggacg aactcgatct tgtcgatcag
     5461 gccgagttcg atcgcggcaa tccgtacctt gcgggcgaat ggcgaggcgg gggagaaggt
     5521 gagtttcatt
//
LOCUS       DASWGM010000683         4788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2687562,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000683 DASWGM010000000
VERSION     DASWGM010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2687562"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /locus_tag="VGA15_19190"
     CDS             <1..1755
                     /locus_tag="VGA15_19190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9589856.1"
                     /translation="LMLLPIYQFHAQRFNANAVLLAAWPLATYCFLRSFETREIRWAV
                     AAGATAALAMLGKYYSVFLIGSFVFAAICHPQRRAYFTSWAPWVSLVTLFTTLLPHLN
                     WLAVNGAPPVVYALTRHTGKDFAPALLEAVLFLLGVAMILAIPAATWALMAQDRVKKF
                     SEDFWAMHSGLLLLFLVSIGTIAFPAITTVALGADMPPLWAFQGLFLFVILIVCGASY
                     PIERFYSANLAVAVIGIAVVAVVVAAPLHAFYRNSHPLHEGRNFFSQAAAELTRRWHE
                     QSDAALAAVGGDDGLALATAFYSPDHPVYQSPLVYPHTETSPREASFSRGWAALCYDV
                     DTNCIDSMERTAARAGRFVRTEFVVQSTLFGQPGASQGFAALIVPPYDEDKITPPPAS
                     SGPAIAQNGSEGASVPVRQDEPTCCTRNPRLDAAERLSEFSLPEISARDSDRKRARRA
                     APPAATTYQANWPDRPASSATRDGFARWPIPTPPPRAKSSVARSSFGARPALQACSAR
                     GPSVSPGREQMADSTSQPLSVRFKRRFCAMAADFDERMRVSQGKFDAFVGNARESVDR
                     RLQLWRRDSMLDQQRRGI"
     gene            complement(1845..2051)
                     /locus_tag="VGA15_19195"
     CDS             complement(1845..2051)
                     /locus_tag="VGA15_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589857.1"
                     /translation="MNQVSRTLEIDDDTGAHLREMAAERGHEVAAALAEVVAMLESVV
                     DLTTWVKSWRSAHELPRPQPRDSN"
     gene            complement(2789..3028)
                     /locus_tag="VGA15_19200"
     CDS             complement(2789..3028)
                     /locus_tag="VGA15_19200"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEX9589858.1"
                     /translation="MMSATKNSRDDRPSLLAAVALLTDLPAQRLARGQVGTIVEQLDD
                     KTSLVEFSDEQGRAYAVAPCQRADLLVLHYVPEAA"
     gene            complement(3025..3363)
                     /locus_tag="VGA15_19205"
     CDS             complement(3025..3363)
                     /locus_tag="VGA15_19205"
                     /inference="COORDINATES: protein motif:HMM:NF045464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589859.1"
                     /translation="MTTRPPNWNEAALDIRKIEDYCLSPSHPRGRHKARVFREALDLQ
                     RSDAGWLRDVLLKAARSSEASQVTVDAWGAHWRLDAAISRQGKSVVVRTIWIVRTGEI
                     APTFVTCWVL"
     gene            complement(3538..>4788)
                     /locus_tag="VGA15_19210"
     CDS             complement(3538..>4788)
                     /locus_tag="VGA15_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9589860.1"
                     /translation="ESVPDIRYWFLDENGLRAISLVVTGVDSNIVGNVASELATQMKR
                     IPLIANVISETSLDRPELRIQPRADLAARLGVSTESLSQTIRVATIGDVGPALAKFDA
                     GDRQVPIRVQLEDGARGDLQLLEQLRVPLGQHGERGGVPLSVVADIKLDQGPTSINRY
                     DRERQATVAADLVGSSALGDATKKIYDLPVMKTLPKGVKVSPSGDAESLAELSDGFAT
                     AITAGLMMVYAVLVLLFGTFLQPITILFSLPLSIGGAIVALLLTGKQLTTPVWIGILM
                     LMGIVTKNAIMLVEFAVESIREGKRRDEAIIDAGMKRARPIVMTTIAMAAGMMPSALA
                     FGAGGEFRSPMALAVIGGLVFSTVLSLVFVPAMFMMMDDVGALFWRFGKKLLASHAEH
                     EQAPAEHGAEHKGPPTIVHSPAAE"
BASE COUNT          789 a         1555 c         1520 g          924 t
ORIGIN      
        1 ctgatgctgc tgccgatcta ccagtttcac gcccagcgct tcaacgccaa cgcggttctg
       61 ctggcggcct ggccgctcgc cacctattgc ttcctgcgct cgttcgaaac gcgcgagatc
      121 cgctgggcgg ttgccgccgg cgccaccgcg gcgctggcga tgctgggtaa atactactca
      181 gtgtttctga tcggaagctt tgtgttcgcc gcgatctgtc atccgcaacg ccgcgcttat
      241 ttcacctctt gggcgccctg ggtttccctg gtcaccctat tcaccacgct gctgccgcat
      301 ctgaattggc tggcggtgaa cggcgcgccg ccggtcgttt atgcgctgac gcggcatacc
      361 ggcaaggact ttgcccctgc gctgctcgaa gcggtgctct ttctcctcgg cgttgccatg
      421 atcctggcga taccggcggc gacatgggcg ctgatggcgc aagaccgcgt caagaaattt
      481 tccgaggatt tttgggcgat gcattcgggc ctgttgctgc tgtttcttgt cagcatcggg
      541 accatcgcgt ttccggcgat cacgacggtc gcgctcggag ccgacatgcc gccgctgtgg
      601 gcatttcagg ggcttttcct gttcgtcatt ctgattgtct gcggcgctag ctatccgatc
      661 gaacggtttt attcggccaa tctggcggtc gccgtcatcg gcattgcggt ggtggcggtc
      721 gtcgtggcgg cgccgctgca cgccttctat cggaacagcc atccactgca cgagggccgc
      781 aatttctttt cgcaggcggc cgcggagttg acacggcgat ggcacgagca gtccgatgcg
      841 gcgcttgccg cggtcggcgg cgacgatggc ctcgccctcg ccacggcttt ctacagcccg
      901 gatcatccgg tctatcaatc gccgctggtc tacccgcata cggaaacctc gccgcgcgag
      961 gcgagcttca gccgcggctg ggcggccttg tgctatgacg tcgacaccaa ctgtatcgac
     1021 tcgatggaaa ggaccgcggc ccgggccggc cggtttgtcc gaaccgagtt cgtcgttcag
     1081 tcgacgctgt tcggtcagcc cggcgccagc cagggcttcg cggccctgat cgtgccgcct
     1141 tacgatgagg acaagattac gccgccaccg gcatcgtcgg gtccggcgat cgcgcaaaat
     1201 ggttcggagg gcgcgtctgt tccggttcgg caagacgagc cgacatgctg cacgcggaac
     1261 ccgcgcctcg acgccgcgga gcgcctgtcg gagttctctc ttccagagat ttccgccagg
     1321 gacagcgaca ggaagcgcgc ccgacgtgct gccccgccgg ccgcgacgac gtatcaggcg
     1381 aattggccgg atcgcccggc atcatccgcc acgcgcgacg gctttgcgcg ctggcctatc
     1441 ccgacgccgc caccgcgcgc gaaatcgtcc gtcgcaagga gcagctttgg cgcaagaccg
     1501 gccttgcagg cttgcagcgc ccgcggaccg tccgtttcgc ccggtcgcga gcagatggcg
     1561 gactcgacgt cgcagccgct gtccgtccgt ttcaaacgcc ggttctgcgc gatggccgcc
     1621 gatttcgacg aacggatgcg cgtttcgcag gggaagttcg acgccttcgt cggcaacgcg
     1681 cgcgaaagcg tcgaccgcag gctgcagctc tggcgcaggg actccatgct cgatcagcag
     1741 cgccggggga tctagttgcc gaagcgtttg aggcacgggg ggcacggcgc gaatgcgcct
     1801 ttgcccgcgc tgcaagtagt caagccctgc aagtcaagct ccggtcagtt gctatcgcgc
     1861 ggttgcggac gaggcagttc gtgcgcggat ctccagcttt tgacccacgt cgtgaggtcg
     1921 acaaccgact cgagcatcgc cacgacttca gccagcgcgg cggcaacctc gtgcccacgc
     1981 tccgccgcca tctcgcgaag gtgcgcgccg gtatcatcat cgatctcaag cgtgcggctg
     2041 acctggttca tggcctaagt ttagcatctg gggcgcgcat gtcgagccgc aggatattga
     2101 cggattattt aggttaacgc cgagcggggc tgtaacatca gcaacgatgc ggcgctgctg
     2161 tctcatcgcg ggtcgagaca tgccggggcg cgcttcaatc gtggcggccc agttcgcctg
     2221 aaagccatcc caacgcatcg gtcttgagag ggtccgccgc gagcctcctt gtttcggaat
     2281 gcccgcactt cgaacattca aatgcgcggc tttcaaggcc gttcggttcg tccgaaacgg
     2341 agatcgttgt cattcggatt tggcacctgg gacagcgcgg gcgctggatc ggcaataatt
     2401 ctggatcggg ttttcgcatg gcattcgccc ttgagcaggc gggagcgcta cactctcatt
     2461 caccgatggc ggccaaagat ctggcggcga taccaaatcc tatttcggac tttgcaattc
     2521 tgtctgtccg gttcccgacg ctaagtccgg atttaagaca caggcacgag tttggcgttc
     2581 gtcatggccg ggcttcgtcc cggccaccca cgtcttatct gccgtgcgat ttgggagacg
     2641 tggatacccg cgacaagcgc gggcatgacg catcacgtgt tgcgatgcct cgcaggcatc
     2701 gcggcccgga tgagtgcaac gaaatccggg acgtcgtcag gttcgctccc ggtcgctgcg
     2761 ctcatgcggg ccacgctcgc ttcgatgctc aggccgcctc ggggacgtag tgcaggacca
     2821 aaaggtccgc gcgctggcag ggcgccacag cataggcacg tccctgttcg tcgctgaatt
     2881 ccaccagcga agtcttgtcg tcaagctgct cgacgatggt gccaacctgc ccacgtgcaa
     2941 gccgctgggc gggaaggtcc gtcaacaatg ccaccgcggc cagaagggac ggcctgtcat
     3001 ccctgctatt tttcgtcgcg ctcatcatag cacccaacac gtgacaaacg tcggcgcgat
     3061 ctcgccggtt cgcacgatcc agatcgttct taccacaaca ctcttgccct gtctgctgat
     3121 ggcggcatcg aggcgccaat gggcgcccca cgcgtccacg gtgacctggg aagcctcgct
     3181 ggagcgtgcg gctttcagca aaacgtcccg cagccaccct gcatcgctgc gttgcaggtc
     3241 aagcgcctcg cggaataccc gagccttgtg gcgaccgcga gggtgcgacg gactgagaca
     3301 ataatcctcg atcttgcgga tatcgagcgc agcctcgttc cagttcggcg gacgtgttgt
     3361 catggcggtt ggatacgcgc ttttccccgc ttcgtcatgc cccgcgaaag cggggcccgc
     3421 agcctcgcgg ttccatcatt gacgtcggcg attactggat catccgcttt cgcggatgat
     3481 gacgtctgtg aatgtggaag agtctttgcg agcccacctg gaagcccgcc cgccccctca
     3541 ctccgccgca ggcgaatgca cgatggtcgg cgggcccttg tgctcggcgc cgtgctccgc
     3601 cggcgcctgc tcgtgctcgg cgtgggaggc cagcagcttc ttgccgaagc gccagaacag
     3661 cgcgccgacg tcgtccatca tcatgaacat cgccggcacg aacaccagcg acagcacggt
     3721 ggagaacacc aggccgccga tcacggcgag cgccatcggc gaacggaatt cgccgccggc
     3781 gccgaacgcc aaggctgaag gcatcatgcc ggcggccatg gcgatggtgg tcatcacgat
     3841 cggacgagcg cgcttcatgc cggcgtcgat gatggcctca tcgcgccgtt tgccttcgcg
     3901 gatcgattcc accgcgaatt ccaccagcat gatggcgttc ttggtgacga tgcccatcag
     3961 catcaaaatg ccgatccaca ccggcgtcgt cagctgcttg ccggtcagga gcagcgccac
     4021 gatggcgccg ccgatcgaga gcggcagcga gaacaggatg gtgatcggct gcaggaaggt
     4081 gccgaacaac agcaccagca ccgcatagac catcatcaat ccggcggtga tcgcggtggc
     4141 aaaaccatcc gagagttcgg cgaggctttc ggcgtcgccc gaggggctta ccttgacgcc
     4201 cttcggcagc gtcttcatca cgggcaggtc gtaaatcttt ttggtggcgt cgccgagcgc
     4261 cgacgagccg acgaggtcgg ccgccacggt ggcctgacgt tcccggtcat aacggttgat
     4321 gctggtcggg ccctggtcga gcttgatgtc ggcgacgacg gagagcggca cgccgccgcg
     4381 ttcgccgtgc tggccgagcg gcacccgcaa ctgctcgagc aattgcaggt cgccgcgcgc
     4441 gccgtcctcg agctgcaccc ggatcggcac ctgacggtcg ccggcatcga attttgccaa
     4501 tgcggggccg acgtcgccga tcgtcgccac gcggatggtc tgcgacaggc tctcggtgga
     4561 gacgccgagc cttgccgcga gatccgcgcg cggctggatc cgcagttcgg gccggtcgag
     4621 cgaggtttcc gagatcacat tggcgatcag cggaatccgc ttcatctggg tcgcgagctc
     4681 gctggcgacg ttgccgacga tgttgctgtc gacgccggtg accacaagcg agatcgcgcg
     4741 caggccgttt tcgtcgagga accagtagcg gatatccggc acgctttc
//
LOCUS       DASWGM010000684         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_344758,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000684 DASWGM010000000
VERSION     DASWGM010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344758"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VGA15_19215"
     CDS             <1..693
                     /locus_tag="VGA15_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9589861.1"
                     /translation="YNYEDMVDAQYRLIKEGLGIKHLRLVIGNSMGGMHTWVWGVRYP
                     QAMDILVPMASQPTEMAARNWMLRRMMIETIRNDPDYNGGNYTAQPRMMKYAINAYQI
                     ATGGGTLGYQTLAPTAAKADQMVDDRLATPVTADANDFLYQWQASHDYNPSPALERIE
                     ATLLAINAADDERNPPETGITDAALKRVKNGRLYLIPASAETRGHLTTGNAKFYTQQL
                     HELLQTAPQRTM"
     gene            725..1072
                     /locus_tag="VGA15_19220"
     CDS             725..1072
                     /locus_tag="VGA15_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX9589862.1"
                     /translation="MSSNRFRRVALGLPEVIEGAHQGHADFRVGKRIFATLGYPDDDW
                     GMVKLTPEQQSMLVEAEPDIFRPVPGGWGKHGNTNVRLARADQARLLSALTLAWTNIA
                     PKLLLATRKKTKP"
     gene            1232..1585
                     /locus_tag="VGA15_19225"
     CDS             1232..1585
                     /locus_tag="VGA15_19225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589863.1"
                     /translation="MRRFAQIALLACLSALAGAALAFDNGQYENVPPDIRAWFKGVIA
                     PNGVPCCDISDGHRTEYDFREGAYWVPIEGQWMAVPERAIIRDRGNPVGEAVVWYVHH
                     RGSIVISCFVPADAV"
     gene            1691..2296
                     /locus_tag="VGA15_19230"
     CDS             1691..2296
                     /locus_tag="VGA15_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX9589864.1"
                     /translation="MTELAASDLETIYPKPSPRVIAKARPEIDAHAKKFIGMSPFCVL
                     ATSGSDGSVDASPRGGNPGFVNVADPNRLLMPDRSGNNRIDSFRNIVEGSGFVQLIFF
                     VPGVDETLRVGGTGKLSAEPDLRAAMEEFGKLPRAVLSIAVHEAYFHCGKALMRAKLW
                     SSETRVERAGFPSISQVIHDQTKLGEPESQAEVEVRYRTQL"
BASE COUNT          480 a          795 c          808 g          437 t
ORIGIN      
        1 tacaactacg aagacatggt cgatgcgcag taccgcctga tcaaggaggg cctcggcatc
       61 aagcatctgc ggctggtgat cggcaattcg atgggcggca tgcacacctg ggtctggggc
      121 gtgagatatc cgcaagccat ggacatcctg gtcccgatgg cctcgcagcc gaccgagatg
      181 gcggcgcgca actggatgct gcggcggatg atgatcgaga ccatccgcaa cgatccggat
      241 tacaatggcg gcaactacac cgcgcagccg cggatgatga agtacgccat caacgcctat
      301 cagattgcaa ccggcggcgg tacgctcggc tatcagacgc tggcgccgac ggcggccaag
      361 gccgaccaga tggtcgacga ccggctggcg acgccggtga cggccgacgc caacgacttc
      421 ctctatcaat ggcaggcgtc gcacgactac aatccttcgc ccgcgctgga gcggatcgag
      481 gcgacgctgc tggcgatcaa tgctgccgac gatgagcgca atccgccgga aaccggcatc
      541 accgacgccg cgctgaaacg cgtcaagaac ggccggctct atctgatccc cgccagcgcc
      601 gagacgcgcg ggcatctcac caccggcaat gccaagttct acacgcagca actgcatgaa
      661 ttgctgcaga cggcgcccca gcggacgatg tgaggcagcg cgcgtctgcg ccgaggtgag
      721 cgccatgtcg tccaatcgct tccgccgcgt tgcgctgggc ctgcccgagg taatcgaagg
      781 cgcacaccag ggacacgccg attttcgcgt gggcaagcgc atctttgcca cgctcggtta
      841 tccggacgac gactggggca tggtcaagct cactccagaa cagcagtcca tgcttgtcga
      901 ggcagagccc gatatcttcc gccccgtgcc cggcggatgg ggcaagcacg gcaacaccaa
      961 tgtgcggctc gcgagagccg atcaagcgag gcttctaagc gccctcacgc tggcgtggac
     1021 caacatcgcg ccgaagttgc tgttggccac gcgcaagaag acgaagccgt gatttcgcac
     1081 cgcatcaatt tgcaggccgg gcgttcgctc attttttgag cgtgcatccg cctgacaact
     1141 ccggcttaat cgcgcgtaat cgcgagattc gtggcgcgcc aaaaaagccg gtccgccacg
     1201 atgatgcgaa tgcagcgctg tggaggatcg catgcgaagg ttcgcgcaga tcgccctgct
     1261 ggcgtgcctg tccgcgctgg ccggcgcggc gctcgccttc gacaacgggc aatacgagaa
     1321 tgtcccaccc gacatccgcg cctggttcaa gggcgtgatt gcgccgaacg gcgtgccctg
     1381 ctgcgacatc tccgatggcc atcgcaccga atatgatttt cgcgagggcg cctattgggt
     1441 tccgatcgaa ggccaatgga tggcggtgcc cgagcgcgcc atcattcgcg accgcggcaa
     1501 tccggtcggc gaagccgtgg tgtggtacgt gcatcaccgc ggcagcatcg tgatcagctg
     1561 tttcgttccc gcagacgcgg tgtgagcttt aagcgccgtg gtcctgcggt gcgagctctt
     1621 gcgagccccg aaggatgacg caaacacttt atgctgatac tctggcggaa accttgagcg
     1681 gagaaagccc ttgaccgaac tcgccgccag cgacctcgag acgatctacc caaagccctc
     1741 gccgcgcgtg attgcgaagg cgcggccgga gatcgacgcg catgcgaaaa aattcatcgg
     1801 catgtcgcca ttctgcgtgc tcgccacgtc cggttcggac ggcagcgtcg atgcgtcgcc
     1861 gcgcggcggc aatccgggtt ttgtcaacgt cgccgacccg aaccggctgc tgatgcccga
     1921 tcgctccggc aacaaccgga tcgacagttt tcgcaatatc gtcgagggct cgggcttcgt
     1981 gcagttgatc ttcttcgtgc cgggcgtcga cgagacgctt cgcgtcggcg gcaccggcaa
     2041 gctgtcagcc gagccggatc tgcgggccgc gatggaggag ttcggcaagc tgccgcgcgc
     2101 ggtactgagc atcgcggtgc acgaggccta tttccattgc ggcaaggcgc tgatgcgcgc
     2161 aaagctgtgg tcatcggaga cgcgggtcga acgcgcaggt tttccgagca tcagccaggt
     2221 gatccacgat cagaccaagc tcggcgagcc tgaaagccag gccgaagtcg aggtgcgcta
     2281 ccggacgcag ttgtgacgaa gaagcttcgc actgccctgc cctgacgttg gctgtcgtac
     2341 cccgcgaaag cggggtatcc agtacgctgc agcatctcga ttccatcgcg actgttctgg
     2401 aatactggat cacccgctgg agcctgtcat cgggcggcgc gttgcgccga cccggtggcg
     2461 ggtgatgacg gctgtgagcg ttgttgttcg cgatgcgcgg cgacccagac agcgagcttc
//
LOCUS       DASWGM010000685         3887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_475041,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000685 DASWGM010000000
VERSION     DASWGM010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475041"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VGA15_19235"
     CDS             <1..519
                     /locus_tag="VGA15_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589865.1"
                     /translation="GDMINAVKQANEFGLTKNQSMVSLLVFISDVHSMELQAAQGLKF
                     VTAFYWDRDEESRAWSTRFLAKQGRMPTMAQASVYSAVRHYIAAIAAAGTDEAKAVLT
                     KMRAMPVNDFYVKNGHLREDGRLVHDMYFAQVKKPSESTKPWDYYNMLGVIPGDQAFR
                     PLAEGGCPLVAH"
     gene            665..1717
                     /locus_tag="VGA15_19240"
     CDS             665..1717
                     /locus_tag="VGA15_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEX9589866.1"
                     /translation="MNHTAALVSLATSVPPHQFHQKQILEAATGLMADRYPQFETLSS
                     LFANTGIHHRYGVKPIEWYLKPRGWPERTEAFLEGAESLFLDVARKAIARADLDPSDI
                     DTVVTVCSTGIATPTLEARVAGKMGFRSDVSRVPVFGLGCAGGVSGLSIASRLAQARP
                     GTNVLLVAIELCSLALRHDELTKANIVAVSLFGDGAAAIILRAGDGGATRIEDAGEHL
                     WPDTLGVMGWSVDPEGFGVIFRRTIPDFVAAHLNPAVVQILARMQLSMADIDRFICHP
                     GGARVITALEAAFSLNQGTLDHERQIIADYGNMSAPTVLFVLERARAKGLPARSLLTA
                     LGPGFTASCVTLRHAT"
     gene            1714..2226
                     /locus_tag="VGA15_19245"
     CDS             1714..2226
                     /locus_tag="VGA15_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9589867.1"
                     /translation="MTSAEFILGLVTLQRAGELVLSRYNTHRLLARGAVEIAPRHYLL
                     IVAVHAAWLISLWVFVHDQPVNVVALILYVVLQGFRFWVMQTLGSRWTTRIIVLPEQP
                     LVSAGPYRFLSHPNYAVVACEIAVLPLVLGLPRLAAVFTILNAAALAIRIRSENRALA
                     ATRQTIVRGA"
     gene            2227..3783
                     /locus_tag="VGA15_19250"
     CDS             2227..3783
                     /locus_tag="VGA15_19250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX9589868.1"
                     /translation="MTERVTEPSTATWLGFVLMCLGMFMAILDIQVVATSLPTIQDAL
                     AISRDAMSWLQTAYLIAEIIAIPLTGWLTRVLTLRWLFVIAISLFTLASIGCAFSGSF
                     PTLVSFRVVQGFAGGALIPAVFSAVFLLFPIRLHPIATTMAGIMAVLAPTVGPVVGGW
                     ITETWSWHWLFLINVAPGIMAAAATPFLLPREKPRFADLATLDGYSLALLAVSLASLE
                     IGLKQAPHDGWLSPLCALLFLLSAAAATAFAARTLKAEHPVVELSTLKNVSFAIGCAL
                     SFCLGVGLFGSVYLMPVFLAYVRRHDAFEIGTIMLVTGVSQLVTAPIAGALEGRLDPR
                     WLSAAGFGLFALGLGSSALQSRVADFDEMFWPQVLRGVAIMFCLLPPTRLALGALTEA
                     QVPDASGLFNLMRNLGGAIGIALIDTILYGRTGGHAEALRDRLISGDITAAQAIGLDL
                     QLFTHRPPDVSDATVEAYLRPMVEKAAFALSANEAWALLACTALLGLLLVPFAGPLPQ
                     RTAADTGNIE"
BASE COUNT          639 a         1245 c         1188 g          815 t
ORIGIN      
        1 ggcgacatga tcaacgccgt caagcaggcg aacgaattcg gtttgacgaa aaaccagagc
       61 atggtttcgc tgctggtctt catttccgac gtccacagca tggagctgca ggccgctcag
      121 ggactgaagt tcgtcaccgc cttctactgg gatcgcgacg aagaaagccg cgcctggtcg
      181 acgcggttcc tcgccaagca aggccgcatg ccgaccatgg cgcaagcgag cgtctattcg
      241 gcagtgcgcc actatatcgc tgcgatcgcc gcggccggaa cggatgaggc caaggcggtc
      301 ctgaccaaga tgcgcgcgat gccggtgaac gacttctacg tcaagaacgg ccatttacgg
      361 gaagacggcc ggttggttca cgacatgtat tttgcccaag tgaagaagcc gtcggaatcc
      421 accaagccct gggattatta caacatgctt ggcgtgattc ccggcgatca ggcgttccgc
      481 ccactcgcgg aaggcggctg tccgctggtc gcgcattgat ccgctcgggg ccatcgttgc
      541 agcaaaggaa agccgcattc acctcatagt gaacgctccc cgctgctcgt gaacacctcc
      601 ggtcgaaata ttcgccgcac tgcgtggttc ataggtcaag gggcatgaga ggcagggaca
      661 ggatatgaac cacacggccg cgctcgtttc gctggcgacc tcggtcccgc cccatcaatt
      721 ccaccagaag caaatcctcg aagccgctac cggcctgatg gcggatcgct atccgcaatt
      781 cgagacgctt tcgagcttgt tcgcgaatac cggcattcat caccgctacg gcgtcaaacc
      841 gatcgaatgg tatctcaagc cgcgtggctg gccggagcga accgaggcct ttctggaagg
      901 cgcggaatct ctgtttctcg acgtcgcgcg caaggccatc gcgcgcgccg atctcgatcc
      961 gagcgatatc gataccgtcg tcaccgtctg ttcgaccggc atcgcgacgc cgacgctgga
     1021 ggcacgtgtc gcaggcaaga tgggctttcg ttccgacgtg tcgcgcgtgc cggtgttcgg
     1081 ccttggctgc gcgggcggcg tttcgggcct gtcgattgcg tcgcgccttg cgcaggcgcg
     1141 gcccggcacc aatgtgctgc tagtcgcgat cgagctatgc tcgctcgcac ttcgtcacga
     1201 cgaactcacc aaggccaaca tcgtcgccgt cagcctgttc ggcgacggcg ctgccgcgat
     1261 catcttgcgc gccggcgatg gcggcgcgac gcgaatcgag gatgctggcg aacacctttg
     1321 gcccgatacg ctcggggtca tgggctggag cgtcgatccg gaagggtttg gcgtgatttt
     1381 ccgccgcacc atccccgatt tcgtcgccgc gcacctcaat cccgccgtgg tgcaaatcct
     1441 ggcgcggatg cagctttcga tggcggacat cgaccgcttc atctgccatc ccggcggcgc
     1501 ccgggtcatc accgcgctgg aggcggcatt ttcactcaat caaggcacgc tcgatcacga
     1561 acggcagatc atcgccgact atggcaacat gtcggcaccc acggtgttgt tcgtgctcga
     1621 acgcgcgcgc gcgaagggac tgccggcaag gtcgctgttg acggcgctcg gacccggctt
     1681 caccgcaagc tgcgtcacgc tgcggcacgc cacgtgacat cggctgaatt cattctcggg
     1741 ctggtcacgc tgcaacgcgc gggagagctc gtcctctcgc gttacaacac tcacagactg
     1801 ctggcccgcg gggcggtcga gatcgcgccc cggcattacc tcttgatcgt cgccgtgcac
     1861 gcagcatggc tgatatcgtt atgggtattc gtgcacgatc aaccggtgaa tgtcgttgcc
     1921 ttgatcctct atgtcgtgct gcagggtttt cgtttctggg tgatgcagac gctcggctcg
     1981 cgatggacga cgcggatcat tgttctgccg gaacaacccc tggtgtcggc ggggccctat
     2041 cgatttctgt cgcatcccaa ctacgccgta gtggcctgcg agatcgccgt actgccattg
     2101 gttctgggtc tgccgcggct ggccgccgtc ttcacaatcc tcaacgctgc ggccctcgcg
     2161 atccgcatac ggtccgaaaa ccgcgccctc gccgcgactc gtcagacaat tgtgcgtggc
     2221 gcataaatga ccgaacgggt taccgagccg agtacggcca cctggcttgg tttcgtcctg
     2281 atgtgccttg gcatgttcat ggcgatcctc gacattcagg tcgtcgcgac atcgctaccc
     2341 acgatccagg atgcactcgc gatatcgcgg gatgcgatga gttggcttca gacggcgtat
     2401 ctgatcgccg agatcatcgc cattccgctg accgggtggc tgacgcgcgt gctgacgctg
     2461 cggtggctgt tcgtcattgc gatcagcctc tttaccctcg cgtccatcgg ctgtgcgttc
     2521 agcggcagct ttccgaccct tgtcagcttc cgcgtggttc agggattcgc cgggggcgcg
     2581 ttgattccgg cggtattttc cgcggtcttc ctgctgtttc cgatccgtct gcacccgatt
     2641 gccaccacga tggctggcat tatggccgtg ctggcaccca cggtcgggcc ggttgtcggc
     2701 ggctggatca ccgagacctg gtcctggcat tggctgtttc tgatcaacgt cgcccccggt
     2761 atcatggcgg ccgccgctac ccctttcctg ctgccgcgcg agaagcctcg tttcgccgat
     2821 ctggctacgc tcgacggata ttccctggcg ttgttggcgg tctcgcttgc aagcctggag
     2881 atcggtctga agcaagcgcc gcacgacggc tggctctctc cgctctgcgc cctgctgttc
     2941 ctgctgagcg ccgccgcggc gactgccttt gcggccagaa cactcaaggc cgagcatccg
     3001 gtggtcgagc tgtcgacgct gaagaacgtc tcttttgcga tcggttgcgc gttgagtttc
     3061 tgcctggggg ttggattgtt cggctcggtc tatctcatgc cggtcttcct cgcttacgtt
     3121 cgacgtcatg acgcattcga gatcggtacc atcatgctcg tcaccggcgt atcgcaactc
     3181 gtgaccgcgc cgattgccgg cgcacttgaa ggccggcttg atccgcgctg gctgtcggcg
     3241 gcggggttcg gtctgttcgc gctcggactg ggtagcagcg cattacaaag ccgcgtcgcc
     3301 gatttcgacg agatgttctg gccgcaagtc ttgcgcggcg tcgcgatcat gttctgcctg
     3361 ctgccgccga cacggctggc gctgggcgcg ctgaccgaag cgcaggtgcc cgacgcaagc
     3421 ggcctattca atctgatgcg aaacctgggc ggtgcgatcg gcatcgcact gatcgatacc
     3481 atcctctatg gacgcaccgg tggacacgcc gaagctttgc gcgaccgtct gatctccggc
     3541 gacatcacgg cggcgcaggc gatcgggctt gatctgcagt tattcaccca tcggcccccc
     3601 gatgtatcgg acgccaccgt cgaggcctat cttcgcccga tggtcgagaa ggcggcgttc
     3661 gcgctcagcg ctaacgaagc ctgggcgcta ctcgcctgta cggcgctgct cggactgttg
     3721 ctcgttcctt tcgcgggacc gctaccgcag cgcactgctg cggacactgg aaacattgaa
     3781 tagcgccacc gggcagaaac gtgcccgccc acacgcggag tgtgctttcc cgctcgccaa
     3841 ggacatttcg gtccggcaat ttcgagggca accggcgtct tcaaata
//
LOCUS       DASWGM010000686         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_344983,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000686 DASWGM010000000
VERSION     DASWGM010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344983"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VGA15_19255"
     CDS             complement(<1..1043)
                     /locus_tag="VGA15_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX9589869.1"
                     /translation="MGKRLIPPEPAEIHEVMLRDALEERYLQYALSTIMHRALPDARD
                     GLKPVHRRILYGMRLLRLDPGTPFKKSAKIVGDVMGSFHPHGDQSIYDALVRLAQDFS
                     SRYPLVDGQGNFGNIDGDNPAAYRYTEARMTEVARLLLEGIDEDGVEFRPNYDGQSKE
                     PVVLPGGFPNLLANGSQGIAVGMATSIPPHNAAELCDAALHLIDKPEAKSKALLKWVK
                     GPDFPTGGIIVDSKESIAEAYMTGRGSFRTRARWVQEEGARGTWVVVITEIPWLVQKS
                     RLVEKIAELLNDKKLPLVGDVRDESAEDIRLVIEPKSRAVDPELLMESLFRLTELESR
                     ISLNLNVLVKGRIP"
     gene            complement(1168..1371)
                     /locus_tag="VGA15_19260"
     CDS             complement(1168..1371)
                     /locus_tag="VGA15_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589870.1"
                     /translation="MFDRTDLVTIATAVSLAGYAAMALWYVALFDIGLTREGVRIAAT
                     VAAILLGAVVAIDFLVRKLAKRK"
     gene            complement(1549..1716)
                     /locus_tag="VGA15_19265"
     CDS             complement(1549..1716)
                     /locus_tag="VGA15_19265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589871.1"
                     /translation="MMIDPKPKLIAALGNAQEYLDLATSAKDRGERAFYERIAELYIK
                     IAQELEALVEG"
     gene            complement(1776..>1961)
                     /locus_tag="VGA15_19270"
     CDS             complement(1776..>1961)
                     /locus_tag="VGA15_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589872.1"
                     /translation="YNYRCPNLECRAEYVAVQRTDASLRKPRCIECATPFLAMQKGLY
                     IQYEAAWPVTLALPDLA"
BASE COUNT          347 a          606 c          616 g          392 t
ORIGIN      
        1 gggatgcggc ccttcaccaa tacgttgagg ttcagcgaaa tccggctttc cagctcggtc
       61 agccggaaca gcgattccat cagcaattca ggatcgaccg cgcgggattt cggctcgatc
      121 acgaggcgga tgtcttcggc ggactcatcg cggacgtcgc cgaccagcgg caacttcttg
      181 tcgttgagca gttcggcgat cttctcgacc agccgggatt tctgcaccag ccagggaatt
      241 tcggtgatga cgacgaccca ggtgccgcgg gcgccttctt cctggaccca tctggcgcgg
      301 gtgcggaacg agccgcgccc ggtcatatag gcttccgcga tgctttcctt ggaatcgacg
      361 atgatgccgc cggtcggaaa atccggaccc ttgacccact tcaacagggc cttcgacttt
      421 gcctcgggct tgtcgatcag atgcagcgcc gcgtcgcaca gctcggccgc gttgtgcggc
      481 gggatcgagg tcgccatgcc gaccgcgatg ccctgcgagc cgttggcgag caggttcgga
      541 aatccgccgg gcagcaccac cggctctttc gactggccgt cgtaattggg ccggaattcg
      601 acgccgtcct cgtcgatgcc ttcgagcagc agccgcgcga cctcggtcat gcgcgcttcg
      661 gtatagcggt aggcggcggg gttatcgccg tcgatattgc cgaaattgcc ctgcccgtcg
      721 accaagggat agcgcgagga gaaatcctgc gccaaccgca ccagcgcgtc gtagatcgac
      781 tggtcgccat gcggatggaa cgagcccatc acgtcgccga cgattttggc ggactttttg
      841 aatggcgtgc cggggtcgag ccgcaacagg cgcatgccat agagaatgcg gcgatgcacc
      901 ggctttagcc catcgcgggc gtccggcagc gcgcggtgca tgatggtcga gagcgcgtac
      961 tggagatagc gctcctcgag cgcgtcgcgc agcatgacct cgtggatttc cgccggctcc
     1021 ggtggaatca gtctttttcc catggggagg agttaaacgc tggaggcgaa tcgggcaagc
     1081 cgcgaatcag gggcggcacg cgcctcattt tcaggcgggc ttggaaactt tgatgattgg
     1141 ccggcctgct ccccccgcgt tcccggctca cttgcgcttg gccagcttcc tgacgaggaa
     1201 atctatcgcg accaccgcgc ccaaaaggat tgcggcgacc gtcgccgcga tgcggacccc
     1261 ttcgcgcgtg agtccgatat cgaataacgc cacataccac agtgccatcg cggcatagcc
     1321 cgccagcgag actgccgtgg caatcgtcac caagtcggtt ctgtcgaaca tcggatactc
     1381 ccgccattat cgtcctcgga ggataggcgg agtgagccca ggtgctgtga tcccactcac
     1441 attgcgcgga ctttccatgg gcgtgccctc gctcagacgg ggagttgccg ggtttgaatt
     1501 agcgaatgct gggacccgtt tgcgacccgt tgcggtaggc gatgcgccct atccctcgac
     1561 cagcgcctca agctcctgcg cgatctttat gtacagctct gcaatacgtt cgtagaacgc
     1621 gcgctcgccg cgatccttgg cgctggtcgc cagatcaagg tattcctgcg cgttgccgag
     1681 agctgcgatc agcttgggct tgggatcgat catcatgaag atactcccgc cgttgctgga
     1741 ggatcgtttc cgcgactagg atcgccagcc ggccatcagg ccaagtctgg cagcgcgagg
     1801 gtgacgggcc acgcggcctc atattggata tacagaccct tctgcatggc aaggaacggc
     1861 gttgcgcatt cgatgcacct cggcttgcgc agggaagcgt cggtccgctg cacggcaacg
     1921 tattcggcgc gacattccaa gtttgggcat ctgtagttgt a
//
LOCUS       DASWGM010000687         8436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_475061,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000687 DASWGM010000000
VERSION     DASWGM010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475061"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..980
                     /locus_tag="VGA15_19275"
     CDS             201..980
                     /locus_tag="VGA15_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9589873.1"
                     /translation="MDLKFSKVTRKGPITIVTLSRPEVYNALHIDAHFELNKIFDDFS
                     TDPEQWVAIVTGAGDKAFCAGNDLKWQAAGGKRGWDTGGFAGLTSRFDCDKPIIAAVN
                     GVAMGGGFEIALACDLIIASENATFALPEPRVGLAALAGGVHRLPRQIGLKRAMGMIL
                     TARHVSAKEGHELGFVNEVVPAGEALAAAERWAETICKNSPMSIRASKQAIQKGLSVS
                     LEQAIAEQREYPAVKAMAASQDYIEGPKAFSEKRPPKWVGK"
     gene            complement(1128..1502)
                     /locus_tag="VGA15_19280"
     CDS             complement(1128..1502)
                     /locus_tag="VGA15_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HEX9589874.1"
                     /translation="MQDEPTPTELIKAVADFLRNDITPMIEGHNAFKLRVGINALDLV
                     TRQLALEPDGDAAEATRLSQLLGLQGSLGELNRALADRIAKGEMDLQTPGLSDHLWQT
                     TMDKLAVDQPNYASYKRELDGK"
     gene            complement(1504..2490)
                     /locus_tag="VGA15_19285"
     CDS             complement(1504..2490)
                     /locus_tag="VGA15_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX9589875.1"
                     /translation="MIEGQLGRRVASWCPGAIGVANSLRLSGGASQETWSFDIVHPDG
                     NIGAILRRAPPGYGASPSRAAGLDAEATLMQLAHDAGLPSPRVMHVLTPQDELGTGFI
                     MQRIEGETIARKILRDAQFAKARPVLARQLGKVAAGIHGLPASKLPKLRQMTATREIA
                     DLEREYRSFNWPRPVFELALRWLRDRDPGPPQEVTLVHGDFRHGNLIIGPEGLRAVLD
                     WELAHLGDPMEDLGWICVNSWRFGEIDRPVGGFGSREELFAGYEGAGRRVDPDRVKFW
                     EVMGTLRWGVMCCGMMQRFRIGPDHSMERAMIGRRSSETEIDLLRLLAPRGK"
     gene            complement(2487..3278)
                     /locus_tag="VGA15_19290"
     CDS             complement(2487..3278)
                     /locus_tag="VGA15_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase"
                     /protein_id="HEX9589876.1"
                     /translation="MQFKHVTLDFDGPVAILKLDHQEVMNAVSIDMLGGLGEALDAID
                     DKRAEVRCLVITGAGRAFCTGANLQGRNNQKPGKSNAGAALETAFHPFLRRLRNLHCP
                     IVTSVNGPAAGAGMSFALMGDMILCARSSYFLQAFRRIGLVPDCGSTWLLPRLIGKAR
                     SVELSLMGERLPAEKGLEWGLVNRVYDDAALMEETMKLAHELANGPTIALSLIRKLYW
                     ESPDNSFEDQLNLEFESQRIAGSAEDFKEGVTAFLEKRPAKFKGK"
     gene            3451..4242
                     /locus_tag="VGA15_19295"
     CDS             3451..4242
                     /locus_tag="VGA15_19295"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9589877.1"
                     /translation="METLDRIFDLTGRVAVITGGNGGIGLGIAQALAAAGCNVSIWGR
                     NADKNRNAAATMAGCAGKVDTRVCDVSDPASVKNAMAGTLDTFGRVDGCFANAGIGGG
                     GRHAFIDRTEEQWRSMFATNLDGVFHVFQAAARHMTERAEAGDRFGRLVATSSLASLF
                     GTARNEHYAATKAAINALVRALAVELARQGVTANAILPGWIKSDMTAGIMANEKFVAN
                     VMPRIPMRRFGEPSDFGGIAVYLMSKASSYHTADCFVIDGGYTAF"
     gene            5014..6165
                     /locus_tag="VGA15_19300"
     CDS             5014..6165
                     /locus_tag="VGA15_19300"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9589878.1"
                     /translation="MLASQRALFEMPRQICYLNSASYSPLPLRTQEAARAAVGRKATP
                     WTLEPSFANNQHERARAAAARLIHADPADMALIPSISYGVATAAKILTIERGARVLVL
                     ENDHSSPVLEWQTRADAQGFAVETIRRPNDGDWTSAVLAAIERSGAPPVGLASISSVH
                     WSDGGLIDIEKVGLALRQRGASFLIDATHSAGVLTMDVRRLDPDFVMFPTYKWLLGPY
                     GRAFLYVARRHQGGIPLEQTAFGRRDVRAENSVYFTDVSYLPDARRFDMGERDHFISM
                     EMASIGMEMMAEWGASAVVQRLQMLTERIAEGLRGFGVSVPERHLRAPHILSLGFRNG
                     MPTGLIEGLAAEGIYVAARLGRMRVSPHVFNDEADADRFVAALAKRLRG"
     gene            6336..6581
                     /locus_tag="VGA15_19305"
     CDS             6336..6581
                     /locus_tag="VGA15_19305"
                     /inference="COORDINATES: protein motif:HMM:NF019324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEX9589879.1"
                     /translation="MNVRSARVDQLAQRLARLTGEDVETALERAIEERLSRVTPPASA
                     DRKAALDSFFERVSQIRVLDPRPTDEIVGYDGCGLPS"
     gene            6563..6883
                     /locus_tag="VGA15_19310"
     CDS             6563..6883
                     /locus_tag="VGA15_19310"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX9589880.1"
                     /translation="MRVAELMVLDTSAIIAAITNEQDSSRFRAAMLGAESLLISSVAV
                     LETKIVLFARLGPDAVGLFEELLENAGIVVVPFDDEMAKVAFDAFRRFGKGQGQPAQL
                     NIVD"
     gene            7093..7494
                     /locus_tag="VGA15_19315"
     CDS             7093..7494
                     /locus_tag="VGA15_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX9589881.1"
                     /translation="MNATLILIDSDAELTRARALVERLWQSDEPADIARLQAQARLIA
                     AYEEGKWPRRPPSTADLIRHLMDQHGLTRADMVPILGTPSRVSEVLQGKKGLSMAMVQ
                     RLRARFNIPADLLLPPPKKAPRSRPTKRAAA"
     gene            7729..>8436
                     /locus_tag="VGA15_19320"
     CDS             7729..>8436
                     /locus_tag="VGA15_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9589882.1"
                     /translation="MKHAYVPRMTTYGLNPDDELNDLRMSDQVRPLYDHVKKFIRETV
                     EPMSVEFFRLGENKQDRWSFTPGQIEVLEKAKAKAKEVGLWNFFLPDDETGAGLKNLD
                     YAYIAAELGKNPLASETMNCSAPDTGNMEVLERVGTPAQKAKWLKPLLNGEIRSAYAM
                     TEPNVASSDAKNISTTAKLVGDEWVINGEKYYISGLGDPRCKIMIVMVKTNPDAPPSK
                     QQSQILVPKDTPGVEIIG"
BASE COUNT         1634 a         2634 c         2595 g         1573 t
ORIGIN      
        1 aagtcttttt tgtcgttagc gcaaggccgc acgaaacagg cccgcgccat tcgattaagg
       61 tgagaacgat agaccaagtc aagcaacgga attgggcttg tgacgcggag gttacctgcg
      121 taattggatg gtatcggacg gtggggccaa gctagtattc aagctacgaa ttcctcgctg
      181 cgaaaaaaac gggagcaaac atggacttga agttttccaa ggtcacgcgc aagggcccga
      241 tcacgatcgt gacgctgtcg cggcccgaag tttacaacgc gctgcacatc gacgcgcatt
      301 tcgaactgaa caagatattc gacgattttt ccaccgatcc ggagcaatgg gtcgcgatcg
      361 tcaccggcgc cggcgacaag gcgttctgcg ccggcaacga tctgaaatgg caggcggccg
      421 ggggaaaacg cggctgggac accggcggat tcgcgggcct cacctcgcgc ttcgactgcg
      481 acaagccgat catcgccgcc gtcaacggcg ttgcgatggg cggcgggttt gaaattgcgc
      541 tggcctgcga cctgatcatt gcctcggaga atgccacctt cgcgctgccc gagccgcgcg
      601 tcggccttgc cgcccttgcc ggcggcgtgc accggctgcc ccgtcagatc gggctgaagc
      661 gtgcgatggg catgatcctc accgcgcgcc atgtctccgc aaaggaaggc cacgaactcg
      721 gttttgtgaa tgaggtggtt ccagccggcg aagcgctggc agccgctgag cgctgggcgg
      781 agacgatctg caagaacagc ccgatgtcga tccgcgcctc caagcaggcc attcagaaag
      841 gcctctcggt ctcgctggag caggcgatcg ccgaacagcg cgaatacccc gcggtgaagg
      901 cgatggcggc gtcgcaggat tacatcgagg gaccgaaggc attttcggaa aagcgcccgc
      961 cgaagtgggt ggggaagtag cgcatcctcg tgtcccggac gcggtgcagc gcgctttgcg
     1021 ctgctccgca gagccgggac ccagattgcc acgctggatg gaccccggat cagcagcgca
     1081 ccacgccgca agggcggcgc gctgcgcagc atccggggaa cgatgcccta ctttccatcc
     1141 aactctctct tatacgacgc atagttcggc tgatcgaccg cgagcttgtc catcgtggtc
     1201 tgccaaagat gatctgatag cccgggcgtt tgcaaatcca tttcgccttt cgcgatgcga
     1261 tcggcgagcg cgcgattgag ttcgccgagc gagccttgca ggcccagcaa ttgcgataat
     1321 cgcgtggctt cagccgcatc gccgtcaggc tccagcgcca gctgccgtgt caccagatcg
     1381 agcgcattga tccctacccg cagcttgaat gcgttgtgtc cctcgatcat cggcgtaatg
     1441 tcgttgcgca ggaaatccgc gaccgccttg atcaattcgg tgggtgtcgg ttcgtcctgc
     1501 atgtcacttc ccccgcggcg ccaagagccg caacaaatca atctcggtct ccgacgagcg
     1561 gcggccgatc atcgcgcgtt ccatcgaatg gtcggggccg atgcgaaagc gctgcatcat
     1621 gccgcagcac atcacgcccc agcgcagcgt gcccatcact tcccagaatt tcacgcgatc
     1681 aggatcgacg cggcggccgg ccccctcgta gcccgcgaat aactcctcgc gcgatccaaa
     1741 tccgccgacc ggtctgtcga tctcgccaaa gcgccacgaa ttgacgcaga tccagccgag
     1801 atcctccatc gggtcgccga gatgcgcaag ctcccagtcc agcaccgcgc gcaacccctc
     1861 ggggccgatg atgaggttgc cgtgacggaa atcgccatgc accagcgtca cctcttgggg
     1921 cggaccgggg tcgcggtcgc gcagccagcg cagcgccagt tcgaacaccg ggcgcggcca
     1981 attgaaactg cgatattccc gctccaggtc cgcaatctcc ctggttgccg tcatctggcg
     2041 aagttttgga agtttcgacg cgggcaagcc atggatgccg gcggcgacct tgccaagctg
     2101 ccgcgccagg accggtcgcg ccttggcgaa ctgcgcgtcg cgcaggatct tgcgtgcgat
     2161 ggtttcgccc tcgatccgct gcatgatgaa gccggtgccg agttcgtcct gcggcgtaag
     2221 cacatgcatc acgcgtggcg acggcaggcc ggcatcgtgt gcgagctgca tcagcgtcgc
     2281 ctccgcatcg agcccggccg cccgcgaggg cgacgcgcca taacccggcg gcgcgcggcg
     2341 caggatcgcg ccgatattgc catcgggatg aacgatatcg aacgaccagg tctcctggct
     2401 ggcgccgccg gaaagcctga gtgagttggc gaccccgatc gcgcccgggc accaggaggc
     2461 tacccgacgt ccaagttgcc cttcgatcat ttgcctttga acttcgccgg gcgcttctcg
     2521 aggaacgccg tgacaccttc cttgaaatct tccgctgagc cggcgatccg ctgcgactcg
     2581 aattcgaggt tgagctgatc ctcgaacgaa ttgtccggac tttcccaata gagtttgcgg
     2641 atcagcgaca gcgcgatcgt cgggccgttc gccagttcgt gcgcgagctt catggtctcc
     2701 tccatcaagg ccgcatcgtc atagacccgg ttgacgaggc cccattccag ccccttctcc
     2761 gccggaagcc gttcgcccat cagcgacagt tcgaccgagc gtgccttgcc gatcagccgc
     2821 ggcagcagcc aggtcgatcc gcagtcgggc acgaggccga tgcggcggaa tgcctgcagg
     2881 aaataggatg agcgcgcgca caggatcatg tcgcccatca gcgcaaaact catgccggca
     2941 ccggcggcgg gaccattgac tgacgtgacg atcgggcagt gcaggttgcg cagacggcgc
     3001 aggaacggat ggaatgcggt ttccagcgca gcacctgcat tgctcttgcc cggcttctgg
     3061 ttgttgcggc cctgcaaatt ggcgccggtg cagaacgcgc gtcccgcgcc agtgatgacc
     3121 agacaacgga cttccgccct cttgtcatcg atggcatcga gcgcctcgcc gagaccgccc
     3181 agcatgtcga tcgataccgc gttcattacc tcctggtgat cgagcttcag gatggcaacc
     3241 ggaccgtcga aatcgagggt gacgtgtttg aactgcatgg gtttcctctg gagttgtgac
     3301 cccgcgttgt cagtgccgcg gtgcgtcagg atttcgatct gaaagccata tttgattttc
     3361 tgggcgagct tgtccatggt tggtatcggc acaactgccc gccatcggac acacggccca
     3421 acgcgcgcca aaccaaaatg gaaacgccca atggaaacgc ttgaccgcat ctttgatctc
     3481 acaggccgcg tggccgttat taccggcggc aatggcggca tcggtcttgg catcgcgcag
     3541 gcgctggctg cggcgggctg caacgtctcg atctggggcc gcaacgcgga caagaacaga
     3601 aacgccgcgg cgaccatggc cggctgcgcc ggcaaggtcg acacccgcgt ttgcgatgtc
     3661 agcgatcccg cttccgtcaa gaacgcgatg gcagggacgc tcgatacatt cggccgggtc
     3721 gatggctgtt ttgccaatgc cggcatcggc ggcggcggac ggcacgcctt catcgaccgc
     3781 accgaagaac aatggcgcag catgttcgcc accaatctcg acggcgtctt tcacgtgttt
     3841 caggcggcgg cacggcacat gaccgaacgc gccgaagcag gagatcgctt tggccgactg
     3901 gtcgcgacct cgagcctggc ctcgctgttc ggcaccgctc gcaacgagca ctacgccgcg
     3961 accaaggccg ccatcaacgc gctggtccgc gcgctcgcgg tggaactggc gcgccagggc
     4021 gtcaccgcca acgccatcct gcccggctgg atcaaaagcg acatgaccgc tggcatcatg
     4081 gccaacgaaa aattcgtggc caatgtaatg ccgcggattc cgatgcggcg gttcggcgag
     4141 ccttccgatt tcggcggcat cgcggtttac ctgatgagca aggcgtcgtc gtatcatacc
     4201 gcggattgtt ttgtgattga tggcgggtat acggcgtttt gagcttactc tcgtcattgc
     4261 gagcgcagcg aagcaatcca tctcgccgcg caaagaaaga aagaatggat tgcttcgctt
     4321 cgctcgcaat gacggccccg attacaaacg cgacttcgcg cctcgcggca caaagcgccc
     4381 gagtcgtgca tttatctctc gcccccctag agggcgtggg gaatgccggg tgcctgatgc
     4441 acccgcggcc tcgtgtgcac tttgtattgg tagaacgcac acgagtaaca acgagtacac
     4501 cagaacgccc ggcattcccg cacgcaatgg ttttacggct tatgtcgtgc tctccccggt
     4561 gaccgggctc ttttgccacc gtcgttagcg gataggtttt gtctaagccc ggttgggccg
     4621 acgcaactcc gcgtacttga cgccagcgtc ggggcgtcag gaccacacga ttttgccgtc
     4681 cgcagcaagc atctctcgtc agcgtgccgg tgatcgctca caagtctttc gacctgccct
     4741 acgatcccat cgcgcgcaaa acgctgccgc gtccaccgca tcccgccccg cgttcgtgac
     4801 gatcatgata cgcccctctt gtggggtggg atggccaagt tctagaagtg atttgggggg
     4861 tacgaaaaca gaaatatttt agcaatcagg actggacgac ccaaatcacc ttgagaagtc
     4921 acaacaaatc gtcctgtgcg cgcatgcggc catatggctg gtacggcccg gataggccat
     4981 aatcggtcct tcccaaccaa gagatccctc aacatgcttg cctcgcagcg cgctttgttc
     5041 gagatgccgc gccagatctg ctacctgaat tcggcctcct acagcccgct gccgctccga
     5101 acccaggagg ccgcgcgcgc cgcggttggg cgcaaggcca caccctggac gctcgagccg
     5161 tcattcgcca acaaccagca cgaacgcgcg cgggctgccg ctgcccgatt gatccatgcc
     5221 gatcccgccg acatggcgct gatcccctcg atcagttacg gcgtcgcgac ggccgcgaaa
     5281 attctgacga tcgagcgcgg cgcgcgcgtc ctcgtgctgg aaaacgatca ttcctcgccg
     5341 gtgctcgaat ggcaaacgcg ggccgacgcg cagggttttg ccgtcgagac gatccgtcga
     5401 ccgaacgacg gcgactggac atcggcggtt ctcgcggcca tcgaacgatc aggtgctccg
     5461 ccggtcggcc ttgcctcgat ctcatcggtg cactggtcgg acggcgggct gatcgacatc
     5521 gaaaaggtag gcttggcgct gcggcagcgg ggcgccagct tcctgatcga cgcgacgcac
     5581 agcgccggcg tgctgacgat ggacgtgcgc cgtctcgatc cggatttcgt gatgtttccg
     5641 acctacaaat ggctgctcgg gccctacggc cgggcctttc tctacgtcgc caggcgccat
     5701 cagggcggca tcccgctcga gcagaccgcg ttcggccgcc gtgacgtgcg tgccgagaat
     5761 tcggtctatt tcacggatgt cagctacctt cccgatgcaa ggcgcttcga catgggcgag
     5821 cgcgaccatt tcatttcgat ggagatggcc tcgatcggca tggaaatgat ggccgaatgg
     5881 ggcgcatcgg ccgttgtgca acgcctgcag atgctgaccg aacggatcgc ggagggcctg
     5941 cgcgggttcg gcgtcagcgt gccggagcgt catttacggg caccgcatat cctgagcctt
     6001 ggctttagga atggaatgcc gaccggcctc atcgagggac tggccgccga gggcatctac
     6061 gtcgcggcgc gtcttggccg catgcgcgtc tcgccccacg tgttcaacga cgaggccgat
     6121 gccgaccgct tcgttgcggc tttggcaaag cggcttcggg gctgatggtt cagcaagtag
     6181 ctggcatgaa gtccgtttcg ggcgctggcg cgctaccacc taggaaaatc ggcctactgt
     6241 cgggcgcaaa tgggcacagg gaaacgaaac gagagtgacc ttggccccca aggatggtat
     6301 atcttcggca ctctcccgct atgacaaggt ggtccatgaa cgtccgttcc gccagggttg
     6361 atcaactcgc gcagcgtctt gcccgcctga ccggcgaaga cgtggaaacc gcgcttgagc
     6421 gtgcgattga ggaacggctg tcccgtgtta cgccaccagc gtccgccgac agaaaggccg
     6481 cgctggattc gttcttcgag cgggtatccc agatacgcgt tctggatccc cgtccgacag
     6541 acgagatcgt aggctatgac ggttgcgggt tgccgagctg atggttctgg atacgtctgc
     6601 gatcatcgct gccatcacca atgaacagga cagctcgcgt tttcgggcgg cgatgctcgg
     6661 cgccgagagc cttttgattt catcggtcgc cgtcttggaa accaagattg ttctcttcgc
     6721 gcgtctggga ccggatgcgg tgggtctttt cgaggagctg ctagaaaatg ccgggattgt
     6781 cgtggtgccg ttcgacgacg aaatggcgaa ggtcgccttc gatgcatttc gccgtttcgg
     6841 caagggccag gggcagcctg ctcagttgaa tatcgtcgat tgagcggtct acgcgctggc
     6901 caaggtgcgt tcccagcctc tcctcttcaa gggtgacgac ttcaatcgga cggatattga
     6961 ttccgccctc gtttgatggt cgagaatggc gggatacggt gtgtagctca aacgggcggc
     7021 tcatcgcgcc ttggcgattc gcttcttcgg cacccacagc gagtacgaca gaatagacgc
     7081 ggagaccgtg tgatgaatgc aaccctgatc ttgatcgata gcgatgcgga actcactcgc
     7141 gcccgcgccc ttgttgaacg actctggcag tcggatgagc cagccgacat tgcccggctg
     7201 caagcgcaag cccgtctgat cgcagcctat gaggaaggca agtggccgcg gcggccacca
     7261 agcacagcgg atctcatccg ccacctcatg gatcaacatg gtctcacccg tgcggacatg
     7321 gtgccgatcc ttggaacgcc gagccgcgtc agtgaggttc tgcagggaaa gaaggggctg
     7381 agcatggcca tggtgcagag attgcgcgcc cgttttaata ttccggccga cctgctgctt
     7441 ccgccaccga agaaagcgcc gcgctcccgc ccgaccaaac gagccgcagc ctgagacgag
     7501 gtgttgggcg ctgtgactct ggattgcttc gctgacgctc gcaatgacgg cgatcgatgg
     7561 aggcgacacc gcgcattgaa atttcgcagg gcggcaaaag ccaaccgacg gaagaagacc
     7621 tcgcctgccc gtctgcttgt aaaggttgat ctgatgtcta gactgccggc aacaagcgct
     7681 tcgcccggcc aaacgggaag caacgatctg cgcgaatggg agtactccat gaaacacgcc
     7741 tacgtgcccc gaatgacgac ctacggcctc aatccggacg atgaactcaa cgacctgcgc
     7801 atgtcggatc aggtccggcc gctttacgac cacgtgaaaa agttcatccg cgaaacggtc
     7861 gagccgatgt cggtcgagtt cttccggctt ggcgagaaca agcaggatcg ctggagcttt
     7921 acgcccggcc agatcgaggt gctggagaaa gccaaggcca aggccaagga agtcggcctg
     7981 tggaatttct tcctgcccga tgacgagacc ggcgccggcc tgaagaatct cgactatgcc
     8041 tatatcgcag ccgagctcgg caagaatccg ctggcctcgg aaaccatgaa ctgttcggcg
     8101 cccgataccg gcaacatgga agtgctcgag cgcgtcggca caccggcgca gaaggcgaag
     8161 tggctgaagc cgctgctgaa cggcgaaatc cgctccgcct atgccatgac cgaacccaat
     8221 gtcgcgtcgt cggatgccaa gaatatctcg acgacggcaa aactcgtcgg cgacgaatgg
     8281 gtgatcaacg gagaaaaata ttacatctcc ggcctcggcg atccccgctg caagatcatg
     8341 atcgtgatgg tgaagaccaa tccggacgcg ccgccgagca agcagcagtc gcagattctg
     8401 gtgcccaagg acacgccggg cgtcgagatc atcggg
//
LOCUS       DASWGM010000688         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2304801,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000688 DASWGM010000000
VERSION     DASWGM010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304801"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1030
                     /locus_tag="VGA15_19325"
     CDS             53..1030
                     /locus_tag="VGA15_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9589883.1"
                     /translation="MNLLRRQFLHLAAGAAALPALSRMSWAQTYPSRPVRLFIGFSAG
                     GGADIVGRLIGQWLQERLGQPVVIENRPGAGTNVATEALVRAPADGYTLGLIGLPNAI
                     NATLYDKLSFNFIRDVTPIAGILRTPEIMVVNLSVPAKTLSEFIAHAKANPGKINMGS
                     AGNGSVSHVAGELFMFKAGLRMVHVPYRGAAPALTDLLGGQVQVVFGAVTATMPYIST
                     GKVRALAVTGTARSELLPDIPTVGELVPGYEASAWYGIGAPKGTPVEIVDRLNTAMNA
                     SLADPKLKARLADLGATVLTGSSADLGKLIADETEKWAKVIEFAKIKAE"
     gene            1140..2693
                     /gene="tcuA"
                     /locus_tag="VGA15_19330"
     CDS             1140..2693
                     /gene="tcuA"
                     /locus_tag="VGA15_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX9589884.1"
                     /translation="MTSAELPGVAPDVLVVGAGNAALCAAISAHQNGARVLMLEAAPF
                     EERGGNSHFTGGAFRFAFAGVDDLLAVLPSLAQERLENVDFGTYTQDQFFDDLFALTQ
                     YRSDPDLLEILVRGSLDTAKWIAQQGVKLQPGLGRQAYQVDGKFKFWGGLALHIWGGG
                     PELLNALYANVERRGIPILYETPATSLIRENGRLAGVIAEHQGAPVAIRAGAVVLACG
                     SFESNPEMRAKYLGPGWEMARVRGTRFNMGAGLNMAMAIGARPYGHWSGSHSVAWDIN
                     APPYGDLTVGDQFQKHNYPFGILINAKGERYVDEGANFHSHTYAKYGGEIIKLPGMFA
                     WQVFDAKVSHLLRSEYRIRRVTKAEANTLEELAGKLDGVDPESFLRTVREFNAACRVD
                     TPFNPNVLDGRRTQGLAINKTNWANPLDTPPFHAYHVTTGVTFTFGGLKISQKGEVED
                     LYGRIIPGLYAAGEMVGGLFFHNYASGTGLMSGATFGRLAGRSAAAFARPPASRAAQV
                     RHDAEGAAP"
     gene            2690..4096
                     /locus_tag="VGA15_19335"
     CDS             2690..4096
                     /locus_tag="VGA15_19335"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9589885.1"
                     /translation="MTMATELARRIRAFTYDGLPDAALEWAKTGILDTVGVTLAGSAE
                     PCAQIMLRVCTATGPALVFGSAQRLSVTDAALVNGTASHALDFDDCSNTLGGHPSVPI
                     LPGLFALADAMPVSGRDFLAAYVAGFETETRIARAVNFHHYDKGWHPTATLGIFGATA
                     AASRLLELTQDQTATALAIAASFSSGLKANFGTMTKPLHVGHCARNGVMAAMLARAGM
                     TASHDVFEHRQGFFNVFNGAGNYDADAVLRDWAEPLDIITPGIAIKSYPCCGSTHPAI
                     DAMLDLVRAHRLTPENVAHVVSWTHPRRLAHTNRPSLRSALDAKFSVQYCLARALVDG
                     QVVLSHFNDDAYLDARVADVMARIEAAPHPQMSSAGTAHFGAEVTVTTTDGRRLVKVV
                     DSALGRTSEQPLPRFALEAKYRDCAGRVLTPDAVDRSLDLVADMETLDRASVLADTLA
                     AGCACDQAGGPAALARAI"
     gene            4234..5112
                     /locus_tag="VGA15_19340"
     CDS             4234..5112
                     /locus_tag="VGA15_19340"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9589886.1"
                     /translation="MIVVPFAAGGGNDILARLIAQHMSRTLGQPFVIENRPGAGGTSG
                     ARAVAKAAPDGHTLMVGHSGVFGIAPALYADPGYDPRRDFAPIGLIASYQQILVVHPS
                     LPVHSVANLIALARKEPGKITYATAGVGSGSHVSTELFAMMADIKLTHVPYRGTGAMQ
                     GDLAGGHVNMSITTFPSVFGQLRSGVLRPIAVTGQTRASILPDLPTVAESGLPGYVAV
                     IHYGLVAPAGLDRAIANRLNVELRNALGSEDVGKRIAEEGGEPLPGTPDQQAIDIGED
                     EAKWGAVVRKLSIKPE"
BASE COUNT          892 a         1664 c         1647 g          913 t
ORIGIN      
        1 ggaaaggtgc gcgcatgaga atgatcgtca tcagagccat gggcctctcg ccatgaacct
       61 tctccgccgc caatttctgc atctggcagc gggtgctgcg gcgctgcctg ctttatcgcg
      121 catgtcgtgg gcacaaacct atccgtcgcg tccggtgcgc ttgttcatag gcttttccgc
      181 cggcggaggg gccgatatcg tcgggcgtct gatcggtcaa tggctgcaag aacggctggg
      241 ccaacccgtc gtcattgaga accggcctgg cgccgggacc aacgttgcca ccgaggcgct
      301 cgtacgcgcg ccggccgacg gctatacgct cggccttatc ggcctgccga acgcgatcaa
      361 cgcgaccttg tacgacaagc tcagcttcaa tttcatccgc gacgtgacgc caatcgcagg
      421 catcctgcgc acgcccgaaa tcatggtcgt caatctgtcg gttccggcca aaaccctttc
      481 cgagttcatc gcccacgcca aggccaatcc gggcaagatc aatatggggt cggccggcaa
      541 tgggagcgtg tcacatgtcg ccggcgagct gttcatgttc aaggccggcc tcagaatggt
      601 gcatgtgcct tatcgcggcg cggcccccgc gctcaccgac ctgctaggcg gccaagtgca
      661 ggtcgtgttc ggggccgtga ccgcgacaat gccctacatc agtacaggaa aggtgcgtgc
      721 gctggcggtc accggcaccg cgcgctcaga actgttgcca gacatcccaa ccgtgggcga
      781 attggtcccg ggctacgagg cgagcgcctg gtacggcatc ggggcgccca aaggcacgcc
      841 cgtcgagatt gtcgatcggc tcaacacggc gatgaatgcg tcgcttgccg atcccaaatt
      901 gaaagcgcgg ctcgcggacc tgggcgccac cgtgctgact ggctcgtccg ccgacctcgg
      961 caagctcatc gctgacgaaa ccgagaaatg ggcgaaggtg atcgagtttg cgaaaataaa
     1021 agcggagtga gcggcgccga cgttctataa ataccgttcg gcgaacccgt ccgcaggacc
     1081 agcgcaatga tcgaacgttt gaagcgccgt tttgtgatcc tcacatctcg ggaggaagca
     1141 tgacatctgc cgaactgccg ggcgtggcgc cggacgtgct ggtggtcggg gcaggcaatg
     1201 cggccttgtg cgccgcgatc tcggcgcacc agaatggagc gcgcgtgctg atgctcgagg
     1261 ccgcgccgtt cgaggagcgc ggcggcaact cacattttac cggcggcgcc ttccggttcg
     1321 cgttcgcagg cgtcgacgat ctcctcgctg tgctgccatc gctggcgcag gagcgtctcg
     1381 agaacgtcga ctttggaacc tacacgcaag accagttttt tgacgacctt ttcgcgttga
     1441 cccaataccg gtccgacccg gatctcttgg aaattctcgt gcgcggcagt ctggacactg
     1501 ccaaatggat cgcccagcag ggtgtgaagc tgcagccggg gttgggacgg caggcctacc
     1561 aggtcgacgg aaaattcaaa ttctggggcg gtctcgcact gcacatctgg ggcggcgggc
     1621 cggagctcct caacgcgctc tatgccaatg tggagcggcg cggcatcccg attctctatg
     1681 agacgcccgc caccagtctc atccgtgaaa acgggcggct cgcgggcgtc atcgcggaac
     1741 accaaggcgc gccggtcgcg atccgggccg gcgctgtggt gctcgcctgc ggcagcttcg
     1801 agtccaatcc ggaaatgcgg gcaaagtacc ttggccccgg ttgggaaatg gcgcgggtgc
     1861 gcggtacgcg attcaatatg ggcgccgggc tcaacatggc gatggcgatc ggcgcaaggc
     1921 cctacggcca ttggtccggt tcccattcgg tggcgtggga catcaacgct ccgccctatg
     1981 gcgatctcac ggtcggcgat cagttccaga agcacaacta cccgttcggc attctgatca
     2041 acgccaaggg cgagcgctac gtcgatgagg gcgccaactt tcacagccat acctatgcga
     2101 aatatggcgg cgagatcatc aagcttccgg gcatgtttgc ctggcaggtg ttcgacgcca
     2161 aggtaagcca tctgctgcgc agcgagtatc gcatccgccg ggtgaccaag gcggaagcca
     2221 acacgctcga agaactggcc ggcaagctcg acggcgtgga tcccgagagt ttccttcgca
     2281 ccgtgcggga attcaacgcc gcctgccgcg tcgacactcc gttcaatccc aatgtcctgg
     2341 atggccgcag gacccaaggc ctcgccatca acaagaccaa ttgggccaat ccgctcgata
     2401 ctccgccgtt ccacgcctat catgtgacga ccggcgtgac ctttacgttc ggcggcctca
     2461 agatttccca gaagggcgag gtcgaggatc tttacggccg gatcattcca ggcctctatg
     2521 cggcgggcga aatggtcggc gggctgttct tccacaacta tgccagcggg acgggcctga
     2581 tgtcgggcgc gacctttggc cggctggcgg gacgcagcgc cgcggcgttc gcgcggccgc
     2641 cggcctcgcg cgcagcgcag gtccgccacg atgctgaagg agctgcccca tgaccatggc
     2701 caccgagctc gcgcggcgca ttcgcgcctt cacgtatgac ggcctgccgg atgccgcgtt
     2761 ggaatgggcg aagaccggca ttctcgatac cgtgggggtc acgctcgccg gatctgccga
     2821 gccgtgcgcc cagatcatgc tgcgcgtgtg caccgccacg gggccggccc tggtgttcgg
     2881 ctccgcgcag cgcctgtcgg ttacggacgc ggcgctggtg aatggcaccg cctcccatgc
     2941 gctcgacttc gacgattgca gcaacacact cggcggccat ccttcggtgc cgatcctgcc
     3001 gggcctgttc gccttggccg acgcgatgcc agtcagcggg cgcgatttcc tggccgccta
     3061 tgtggcgggc ttcgagaccg aaacgcgcat cgcccgcgcc gtcaacttcc accattacga
     3121 caagggctgg cacccgaccg caacgctggg catattcggc gccaccgcag cggcctcgcg
     3181 gctcctcgag ctgacgcagg atcagaccgc aaccgcgctc gccatcgcgg cttcgttttc
     3241 gtccggcctc aaggccaact tcggcaccat gaccaagccg ctgcatgtgg gtcactgcgc
     3301 ccgcaacggc gtgatggccg cgatgctcgc gcgcgcgggt atgacggcga gccacgatgt
     3361 gttcgagcac cggcaaggct ttttcaatgt cttcaacggc gccggcaact acgatgccga
     3421 cgccgtgctg cgcgactggg cggaaccgct cgacatcatc acgccgggca tcgccatcaa
     3481 gagctatccg tgttgcggca gcacccatcc ggccatcgac gccatgctcg atctggtgcg
     3541 cgcgcatcgc ctgacgcccg agaatgtcgc tcatgtggtg tcctggacgc atccgcgacg
     3601 tctcgcgcac accaaccgtc cgagcctgcg cagcgcgctc gatgcgaagt tcagtgtgca
     3661 atactgcctg gcccgcgcgc tggtggacgg ccaagtggtg ctgtcgcatt tcaacgacga
     3721 cgcttatctc gacgcgcggg tggcggatgt gatggcccgc atcgaagcgg cgccgcatcc
     3781 gcagatgtca tccgccggca ccgcgcattt cggtgccgag gtgacggtca cgaccaccga
     3841 cggacgacgc ttggtgaagg tggtggacag cgcgctcgga cgcacgtcgg aacagccgct
     3901 gccccggttt gccctcgagg caaagtatcg cgattgcgcc ggccgtgtgc tgacccctga
     3961 cgcggtggac cggtcgctcg atctggtggc ggacatggaa acgctcgacc gcgcgtccgt
     4021 gcttgccgac acactggccg cgggatgcgc ctgcgatcag gcgggcggac ctgccgccct
     4081 ggcgcgggca atctgacagt ttctgacttc ggtttgcgca ccatgaaaaa tttacggagc
     4141 acaaggtcac tcatcgcgag cctcgcggca gcgccgatca tgatggccgc ggcctggccg
     4201 gcgatggctc agacctatcc gacgcgtccc gttgtgatcg tggtgccgtt tgcggcgggg
     4261 ggaggcaacg acattctggc gcgcttgatt gctcagcaca tgagccgaac gctgggccag
     4321 ccgttcgtga ttgagaatcg ccccggcgct ggcggtacca gcggagcgcg ggccgtcgcc
     4381 aaagccgcgc ctgacggcca taccctgatg gtcggccatt cgggcgtgtt cggtattgcg
     4441 ccggcccttt acgccgatcc gggttatgat ccgcggcggg atttcgcacc gatcggactg
     4501 atcgcctcgt atcagcaaat ccttgtggtc catccgtcgc tgccggtgca cagcgtcgcg
     4561 aatctgattg cgctggccag gaaagagccc ggcaagatta cctatgcgac ggccggagtc
     4621 ggctcgggtt cgcacgtctc gacggagctg ttcgccatga tggccgacat caagctcacc
     4681 cacgtcccct atcgcggcac tggcgccatg caaggcgatc tcgcgggtgg ccatgtcaat
     4741 atgtcgatca ccacattccc ctccgtgttc gggcaactgc gcagcggggt cctgcgcccg
     4801 atcgccgtga cgggccaaac acgcgcctcg attcttcccg acctgccaac cgtcgcggag
     4861 tccgggctgc ccggttacgt cgcggtgatt cactacggcc tggtggcgcc ggctggactt
     4921 gatcgcgcca tcgcgaacag gctcaacgtt gagctgcgca acgcgcttgg cagcgaagac
     4981 gttggtaaac gcatcgccga agagggcggt gagccgctcc ccggcacgcc agaccaacag
     5041 gccatcgaca tcggcgagga cgaggcgaag tggggcgcgg tggtgcggaa gttgagcatc
     5101 aagccggaat gatggc
//
LOCUS       DASWGM010000689         3935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1756812,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000689 DASWGM010000000
VERSION     DASWGM010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756812"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..2230
                     /locus_tag="VGA15_19345"
     CDS             95..2230
                     /locus_tag="VGA15_19345"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX9589887.1"
                     /translation="MLQNLTEEVHRKTHPDSTLPWIGAIGLAFAVGIAYFLAAHLSLA
                     LLAEPDGVAVFWPAAGVSSGVLIALGRDARLPVAGGAMVATVIANLMGDRNVWSATAF
                     ALCNAGEALLAAWLIERYFGSNFSLDRLRNVLGLLAAAVVATAASGVGGTVAYRLFHS
                     PTAPLWTTWQHWFASDAVGIITVAPLVIGLAKALREPPPRNEILEGVVALVALAAVMT
                     VIIVLLPPEPWQTVHPAALLLPILLWLTARCQPVFAAAAAFIVSLTTVWTITFGIGHF
                     GDPGLPIGDRILDAQAAIVAFTLYAYVLAALFAERRQSETRLQEALAAGAVTAFEWNP
                     RNGLSQRSKNAAQILGLGPQQTFTAAQFLSRVHPDDRARFKALVDSVRVDSPSYSVTF
                     RFIRPDGREVWLEETSRAEFDTAGRVVGLKGLTRDITRRKQSEKRQDLLIAELDHRVK
                     NVLARVAAVVMHTRHRCGTMDEFVKALHGRIQSMAAAHALLSQSRWSGVGLTDLIRHQ
                     LAPYTTDANTTISGPEIMLTSAQTQAVAMVIHELVTNAAKYGALSSPDGRVSASWHRT
                     GADVAAILTITWRELGGPPIAAPFQSGYGSGLIRDLIPHELGGTVDLVFSSDGACCKI
                     EIPFEGGAKVPREEALRRAIMDYDALNTGVRKFFKKLRVTAQREIEKAVRDADAKRKL
                     TGTTLPTKAVVTVGGVDLKFEIDGDIELA"
     gene            complement(2908..>3935)
                     /locus_tag="VGA15_19350"
     CDS             complement(2908..>3935)
                     /locus_tag="VGA15_19350"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9589888.1"
                     /translation="VTRNGGTVMNDKQLAGPWVRRFLLEYLVAERNLSGNTQASYRDT
                     LTLLLPFASASVGHAIDRMTVEDLTPSIVRQFLDHLERDRHCSGVTRNQRLAMIHSLA
                     RFIGTHSPVHLAWCTELRSIPFKKTAKTSIGYLEKHEIDALLNQPDQRTVLGARDHFL
                     LLFLYNSGARADEAAKLTIGDLQLGRSSPSVRILGKGNKIRMCPLWPATTVLLSRLVI
                     GRAAKEAVFLSRTNQPLTRFGIHRLVTHYARLAAKSVPSMIGKHISPHTIRHTTAVHL
                     LRAGVDINTIRAWLGHVSLDTTHVYAEVDMEMKAKALASVDISGLHHRPQRGSALPSL
                     MAFLKGL"
BASE COUNT          733 a         1203 c         1212 g          787 t
ORIGIN      
        1 catcctccgc gtgtctttga cagatttcgc gtcattcccg ttcgctggta gaattgtcgt
       61 tcggtacatt ggattaagaa tttttggggg tcaaatgctg cagaatctaa ccgaggaagt
      121 tcaccgcaaa acccaccccg actcgacgtt accttggatt ggtgcgatcg ggctcgcctt
      181 tgcggtcggc atcgcctatt tcctcgcggc gcatctgagc cttgcgcttc tcgccgaacc
      241 tgacggcgtt gccgtgttct ggcctgctgc gggcgtgtcc tccggcgtcc tgatcgcgct
      301 tgggcgcgat gcgcgattgc cggtcgctgg cggcgcgatg gttgcgacgg tcattgccaa
      361 tctgatgggc gaccggaacg tctggagcgc cactgccttc gctctatgca atgccggcga
      421 agccctgctt gccgcatggc tgatcgagcg ctatttcggt tcgaatttca gcctcgacag
      481 gctacgcaat gtgctgggtc tgctggcggc tgcagtcgtc gcaaccgccg cctcgggtgt
      541 cggcgggaca gtggcttata ggttgttcca cagcccgacg gcgccgcttt ggaccacttg
      601 gcagcattgg tttgcgtccg acgccgtcgg tatcatcact gttgcgccgc tggtgatcgg
      661 cctcgccaaa gccctgcgcg agccgccgcc gcgcaacgag atcctcgagg gcgtcgtagc
      721 gcttgtggca ttggcggcgg tgatgaccgt catcatcgtt ttgctgccgc cagagccgtg
      781 gcagacggtg catccggccg ccctgctgct tccgatcctg ctgtggctta cggctcgttg
      841 ccagccggtg ttcgctgcgg cagcagcgtt catcgtttca cttacgactg tttggacgat
      901 aacctttggc atcggtcatt tcggggatcc cggccttcca atcggcgacc gcatcctgga
      961 tgcccaagcg gccatcgtcg cctttacgct atacgcatac gttctcgcgg cgctgtttgc
     1021 cgagcggcgt cagagcgaga cgcggttgca ggaagcgctg gcggccggag cggtgacggc
     1081 cttcgagtgg aatcctcgca acggcctgtc gcagcgcagc aagaacgctg cgcagatcct
     1141 gggccttggc ccgcagcaga ctttcaccgc cgcacaattc ctctcgcggg ttcatccgga
     1201 cgaccgcgca cgtttcaagg cgctcgtcga tagtgtccgc gtcgacagcc cctcctattc
     1261 tgtcaccttt cgtttcattc gcccggacgg gcgggaagtg tggctcgagg aaacgtcaag
     1321 agcagaattc gacacggccg gccgcgttgt aggcctcaaa ggcctgaccc gcgatatcac
     1381 gaggcgcaag cagtctgaaa agcgccagga tctgctgatc gccgaactcg accaccgcgt
     1441 caaaaacgtg ctggcgcgcg ttgcggcggt cgtcatgcat acgcgccacc gctgcgggac
     1501 catggacgag tttgtcaaag cgctccacgg tcgcattcaa tcgatggcgg ccgcccacgc
     1561 acttctgagc caaagccgct ggtccggcgt gggccttacc gacctcatac gccaccagct
     1621 tgcgccctac acgaccgacg caaatacgac gattagcggc ccggagatca tgctcacctc
     1681 tgcgcagacc caggcggtcg ccatggtcat tcacgagctg gtgacgaatg ctgcgaagta
     1741 cggcgcccta tcgagccccg acggcagagt gtcggcgagc tggcatcgca ccggcgccga
     1801 cgtggcggcg atcctgacaa tcacatggcg cgaacttggc ggcccgccga tcgcggctcc
     1861 gttccaatcc ggttatggca gcggcctcat ccgcgatctc attcctcatg aactcggcgg
     1921 taccgtcgac ctggtattct catccgacgg cgcgtgctgc aaaattgaaa ttcccttcga
     1981 gggaggcgct aaagttcccc gcgaggaagc gctaaggaga gcgattatgg actacgacgc
     2041 cctcaacacc ggcgtacgca agttcttcaa gaagctccgc gtcactgccc aacgagagat
     2101 cgagaaggcg gttcgcgacg ccgatgcgaa gagaaaactg accggcacca cattgccgac
     2161 caaggcggtc gttacggtcg gcggcgtcga cctcaaattc gaaatagacg gcgatatcga
     2221 attggcgtga agaacgtcat cgctaccgtg gcgtttaagc tccgctgcat ccaatactca
     2281 gattagcggt tttcccacgc gctcagcaaa catcggaggc gctcggcgcg caggtgaagg
     2341 ctgacatcga gaaatggtgg ccgatcatca agcggtccgg catcaagccg cagtgatcca
     2401 gcgaagtatt ccttaaatcc cgttcgccga atcctgcgtg cccaatgtct cttacgggtc
     2461 aatcagcgcc atcgaacttc gtggccgtca cggaggaact tgcttcgata gctgacgcgg
     2521 gacggcacga atcccggttc cgggcggcga gcaccagggg aaggccgcag gaccccagaa
     2581 accggccgcc gcggcggacg gccgtggctt cagccttggg ccaccagcag acgttgcctg
     2641 gttggcgaag acgccgttaa ttcatcttgg tcactgccgg tggcgtccag gagccatcgg
     2701 tgattgcgac atttggccag taggcgcgga ggcagagtga aaaatcgccg ctcttgggcg
     2761 ccggcagcca gttatcaacc tgccccgcgg actaccgcta ctcgcgttat gttgcgccgg
     2821 gatttatgtt gcgcggcggg cgacgggcca gggatgccgc tctttccgct ggcccgcgcg
     2881 gcacataagg aggacggtgc tgccgcccta gagtcccttc aggaatgcca tcaacgaggg
     2941 gagcgcgctg ccccgctgcg gccggtggtg cagaccggag atgtcgacgc tcgctaacgc
     3001 cttggccttc atctccatat cgacctccgc atagacatgc gtggtgtcga gcgagacgtg
     3061 acccagccag gctcggattg tgttgatgtc gacgccggca cggaggagat ggacggccgt
     3121 cgtgtggcga attgtgtgcg gactgatgtg tttgccgatc atcgacggta ccgacttggc
     3181 ggccaacctt gcatagtgcg tgacgagacg gtgtatcccg aaccgagtca acggctggtt
     3241 tgtccgactc aggaagactg cttctttcgc ggcccgcccg atcaccaagc gcgatagcaa
     3301 aactgtcgtt gccggccaca ggggacacat tcgaatcttg ttccccttgc caaggatgcg
     3361 gactgacgga gaggaccttc ccagttgcag gtcgccgata gtcagttttg ccgcctcgtc
     3421 ggcccgcgcg ccgctgttat ataagaagag caaaagaaag tggtcgcgag ccccaagcac
     3481 agtgcgttga tcaggttggt tcagcaaggc atcaatctcg tgcttttcga ggtaaccgat
     3541 cgacgtcttg gcggtcttct tgaacggaat tgatcttagc tcggtacacc aggcgaggtg
     3601 aactggcgag tgcgtgccaa tgaaccgcgc cagagaatgg atcatggcaa gcctctgatt
     3661 acgcgtgacg ccgctgcaat ggcgatcgcg ttccaggtga tcaaggaact gacgaacaat
     3721 ggacggtgtc aaatcctcga ccgtcatccg atcgatggca tgcccgacgc tcgcactggc
     3781 gaaaggcagc agcagggtca gcgtgtcacg atagctggct tgggtattgc ctgagagatt
     3841 gcgctcggcc accaggtact ccaggaggaa gcgccggacc cagggcccgg caagctgctt
     3901 gtcattcatg acggttcccc cattgcgagt aaccg
//
LOCUS       DASWGM010000690         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_345369,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000690 DASWGM010000000
VERSION     DASWGM010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345369"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="VGA15_19355"
     CDS             complement(<1..1679)
                     /locus_tag="VGA15_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX9589889.1"
                     /translation="MNHDVHQVRSFEHPGEGRRRAKSTPKGRQVDPQATEEIAVLLGD
                     RPRRRDLLIEHLHLIQDKYRQISAAHLAALADEMKLAFAEVFETATFYAHFDVVKEGE
                     ANIAPLTIRVCDSLTCAMLGAEKLLHELQDGAGPGIRVVRAPCVGRCDTAPAAEVGHH
                     FVDHASVAGVLAAAKGGDTHAHLPDYVDYDAYVAGGGYTLLKRLHSGELTKEDILKAL
                     DDASLRGLGGAGFPAGRKWRAVLGEPGPRLMAINGDEGEPGTFKDRYYLETDPHRFIE
                     GMLIGAHVVEAPDIYIYIRDEYPASREILERELAKLPPGGPTLHMRRGAGAYICGEES
                     SLLESIEGKRGLPRHKPPYPFQVGLFGLPTLINNIETLWWVRDIVEKGAEWWKSHGRN
                     DRHGLRSYSVSGRVKNPGMKLAPAGIAVRELIDEFCGGMADGHSFHAYLPGGASGGIL
                     PASMDNIPLDFGTLEKYGCFIGSAAVIILSDQDSVKGAALNLMRFFEDESCGQCTPCR
                     VGTQKAALLMEKPVWDRELLDELSQAMRDASICGLGQAASNPLTTVIKYFP"
     gene            complement(1832..2104)
                     /locus_tag="VGA15_19360"
     CDS             complement(1832..2104)
                     /locus_tag="VGA15_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589890.1"
                     /translation="MLRTSVCLAAMLLVVGALHPASAQTAAPATTPTPAAKPSKMKLT
                     ADRLKEMKAKWSANKGKLAVCRKEVKSKGLAGDDRWFYIEDCMGKT"
     gene            complement(2200..>3069)
                     /locus_tag="VGA15_19365"
     CDS             complement(2200..>3069)
                     /locus_tag="VGA15_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9589891.1"
                     /translation="NNVHLNDAHKTPQFWLIWGVLCLNVSAGIGVIGMASPMLQEIFA
                     GKLIGSPDVGFNALSVEQKATIAAIAAGFAGMLSLFNIGGRVFWASLSDYIGRKNTYF
                     CFFLLGILLYALAPTFAVMGSKLLFVGAFCIILSMYGGGFATVPAYLADMFGTQFVGA
                     IHGRLLTAWSTAGIIGPVVVNYIREFQLAAGVPRDQLYNTTMYILCAMLVGGLICNYL
                     IKPVDHKWHMSTEEVSMLQAASAKSEAAVQHGSFGIGKGGLDAKAALFWAFVGIPLAW
                     GVWKTLESAMKIF"
BASE COUNT          590 a          985 c          913 g          581 t
ORIGIN      
        1 tccggaaaat atttaatgac tgttgttagc ggattcgagg ccgcctggcc gagaccgcag
       61 atcgacgcat cgcgcatcgc ctggctcaat tcgtcgagca actcgcggtc ccagaccggt
      121 ttttccatca gcagcgccgc cttctgggta ccgacgcggc agggcgtgca ctggccacag
      181 ctttcgtcct cgaaaaagcg catcagattc agcgccgcac ctttcacgct gtcctggtcg
      241 gacaggatga tgacagcggc ggaaccgatg aagcagccgt atttttccag cgtgccgaaa
      301 tcgagcggaa tattgtccat cgatgccggc aggatgccac cggaggcgcc gcccggcaga
      361 taggcgtgga agctatgtcc gtcagccatc ccgccgcaga actcatcgat cagctcgcgc
      421 acggcgatgc cggccggcgc cagcttcatg ccgggatttt ttacccgccc cgacaccgaa
      481 tagctgcgca ggccgtggcg atcattgcgg ccgtggctct tccaccactc cgcgcctttt
      541 tcgacgatat cgcgcaccca ccacagcgtc tcgatgttgt tgatcagcgt cggcaatccg
      601 aacagcccga cctggaacgg ataaggcggc ttgtgccgcg gcagtccgcg cttgccttcg
      661 atgctttcca agagcgagga ttcctcgccg cagatatagg cgccggcgcc gcggcgcata
      721 tgcagcgttg ggccgcccgg cggcagtttt gcaagctcgc gttcgagaat ttcgcgagaa
      781 gccggatatt cgtcgcggat gtagatgtag atatccggcg cctccaccac atgcgcgccg
      841 atcagcatgc cctcgatgaa gcgatgcgga tcggtttcga gatagtagcg atccttgaac
      901 gtgccgggct cgccctcgtc gccgttgatc gccatcagcc gcggccccgg ctcaccgagc
      961 accgcgcgcc actttcgccc cgccggaaaa cccgcgccac cgagaccgcg caaggacgcg
     1021 tcgtcgagcg ccttcagaat atcttccttt gtcagttcgc cggagtgcag gcgcttcagc
     1081 agcgtatagc cgccgcccgc gacataggcg tcgtagtcta catagtcggg caggtgcgcg
     1141 tgggtgtcgc cgcccttcgc cgcggcgagc acgcctgcaa cgctggcgtg atcgacaaag
     1201 tgatgaccga cctcggccgc aggcgcggta tcgcaacggc cgacgcaagg cgcgcgcacc
     1261 acgcgaatgc ccgggccggc gccgtcttgc agctcatgca gcagtttttc cgcacccagc
     1321 atggcgcagg tcagggagtc gcagacgcga atggtcaacg gcgcgatatt ggcctcgccc
     1381 tccttcacca cgtcgaaatg cgcataaaag gtcgcggtct cgaacacttc cgcaaacgcc
     1441 agtttcatct cgtcggcaag ggctgcgagg tgggctgcgg aaatctgacg atatttgtcc
     1501 tggatcagat gcagatgctc gatcagcaga tcgcgccgcc gaggccggtc acccagcaaa
     1561 accgcaatct cttcagtggc ttggggatcg acctggcgtc ccttgggggt cgatttcgcc
     1621 cgccttcgtc cctcgccggg atgctcgaac gagcggacct gatgcacatc atgattcatc
     1681 gaaacgtctc gttcatgttt cagccatccg actctaatcc gtggcatgcc acatgccaag
     1741 cagaataatg ctgtgcccat aagcatttaa ggggcacgca gctctcgctg tcatgcccct
     1801 cggtagacgg caattggcgg aaccgccgtc ctcaggtctt gcccatgcag tcttcgatgt
     1861 agaaccagcg atcgtcaccg gccagcccct tcgacttcac ctctttgcga caaactgcca
     1921 gcttgccctt gttcgcactc cacttggctt tcatttcctt cagcctgtcg gccgtcaact
     1981 tcatttttga aggctttgcg gctggagtcg gcgtcgttgc tggcgccgcc gtctgggccg
     2041 acgcgggatg gagcgcgccc acgacaagca gcatggcggc gagacaaaca ctggtgcgaa
     2101 gcataggatt tccccccgtt tttcatttct ttgattgatt gcaagggcaa aagaacgcgg
     2161 ggcgcgcgac cgttggcgtc ccgcggcgat gagcggcgat cagaagattt tcatcgcgct
     2221 ttccagcgtc ttccatacac cccaggcgag cgggatgccg acgaaggccc agaacagcgc
     2281 ggccttggcg tcgagcccgc ctttgccgat gccgaatgag ccgtgctgca ccgcggcctc
     2341 gctcttcgca ctcgccgcct gcagcatgga gacctcttcg gtgctcatgt gccatttgtg
     2401 gtcgaccggc ttgatcaggt agttgcagat cagcccgccg accagcatgg cgcacaggat
     2461 gtacatggtg gtgttataga gctggtcgcg cggaacgccg gccgcgagct ggaactcgcg
     2521 gatatagttg acgaccaccg gaccgatgat gcccgccgtc gaccacgccg tcagcagccg
     2581 cccgtgaatg gcgcccacga actgcgttcc gaacatgtcg gcaagatagg ccggcacggt
     2641 ggcgaagcca ccgccataca tcgacaggat gatacagaac gcgccgacaa acagcagttt
     2701 ggagcccatg acggcgaagg tcggcgccag cgcgtagagc aagatgccga gcaggaagaa
     2761 gcagaaatag gtattcttgc ggccgatata gtccgacagc gacgcccaga acacccggcc
     2821 gccgatgttg aacagcgaca gcattccggc gaagccagcc gcaatcgcgg cgatggttgc
     2881 cttctgctcg acgctcagcg cgttgaagcc gacgtctggc gatccaatca gtttgccggc
     2941 gaatatctcc tgcaacatcg gcgaggccat gccgatcacg ccgatgccgg ccgacacgtt
     3001 caggcagagc accccccaga tcagccagaa ttgcggggtc ttgtgcgcgt cgttgagatg
     3061 aacgttgtt
//
LOCUS       DASWGM010000691        18911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_194086,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000691 DASWGM010000000
VERSION     DASWGM010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18911
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194086"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VGA15_19370"
     CDS             <1..382
                     /locus_tag="VGA15_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEX9589892.1"
                     /translation="RAREMFSYAASYFGNADAQYDLARLYLRGLSSSREDFRYGARWL
                     GLAAQKGQHQAQAMLGQMLFNGDQLPRQAARGLMWLTLARDSATPDETWIKESYNKAI
                     AKASDDDRAMALQMLEHWVQGRKD"
     gene            complement(433..1224)
                     /gene="xth"
                     /locus_tag="VGA15_19375"
     CDS             complement(433..1224)
                     /gene="xth"
                     /locus_tag="VGA15_19375"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX9589893.1"
                     /translation="MRIATWNVNSIRQRLDHLLTWLQDCTPDIVCLQEIKCVDEAFPR
                     EAIEALGYNVVTHGQKTFNGVALLSKLPFDETRPRLAGDDEDIHARFLEGVVTLKHGV
                     LRIACLYLPNGNPPETDKYPYKLRWMSRLHEYSKQRSKTEEPLVLAGDFNVIPAAQDV
                     HNPAAWVNDALFRPATRESFQSLLGLGLTDALRAVTDGSGQYTFWDYQAGAWQKNHGL
                     RIDHLLLSPQASDRLIDVGIDSYVRAWEKPSDHVPVWIDLDLETA"
     gene            1384..2514
                     /locus_tag="VGA15_19380"
     CDS             1384..2514
                     /locus_tag="VGA15_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9589894.1"
                     /translation="MKKRNWIILAGGLGIIALAAFATRASWMGGGASAQGPQRARVIS
                     VELAKAERKPVPVDVDSIGTVTPISSVALKSRVETTIVSVHFEDGAKVSEGDLLFTLD
                     ARQIDAQIEQAEGMLAKDQAQLEGAQRDLRRFSDLIGKGATTQVNVDNAKTQADILIG
                     TIKADQSALDNLKVQKSFTIIRAPFSGRISAANVKIGNFVRPADTAPLAVINQMAPVY
                     VTFAIPQRVLVDLRDAMATGDSRVIATIPGHERSEGGKVAMVENSVDSTTGMITVRGI
                     MNNANETLWPGILVNTKLIIRTEEAVTVPSAAVQRSQNGNFVFIMRDGKAHVEPVSVS
                     RTSQGMSVIASGLSGGEDVIVDGQLLLSEGTPVAPRAPKAGA"
     gene            2518..5646
                     /locus_tag="VGA15_19385"
     CDS             2518..5646
                     /locus_tag="VGA15_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9589895.1"
                     /translation="MTLSELCIRRPVMTTLMTASIIAFGVFGFRLLPVSALPKVDFPT
                     IAVTATLPGASADTMAASVAGPIERQLSTIAGISSMSSSSSQGLSVITIQFDLNRNID
                     GAALDVQTALTIAQRRLPIEMTIPPSFRKVNPADFPVLFVTLGSTTLPLSAVNEYGDI
                     TVGQALSQLPGVAQVTIFGAQKFAIRVQADPEAAAARGLSLEDIRAAVSRANSSTPVG
                     TLNGPKQDIALVASGQMDRAADYRHIVVAWRNGSPVKLDEVAHIYDSVENDKIATWLN
                     DRRAIVLAIQKQPDANTVAVVDGVRAKLPSLRAQIPPSVVMEVLLDRSVSIRQSVSDV
                     EETLLIAVALVIMVIFLFLRSASATFIPALAVPISLLGTCAVMYMLDYSINNMTLLAL
                     TLSVGFVVDDAIVMLENIVRHIEHGTKPYEAALKGAREIGFTIISITFSLIAVFIPVL
                     LMGGIVGRVFREFAVTIAVAIVVSGFVSLTLTPMLCARVLKAHDETRRPNIVLRVFER
                     IFDSWLRGYEWALDRVLARKAMMLVVTFATLAGTVALYVVVPKGFFPQEDTGFLFGTT
                     EAATDTSFEAMKDRQGALVAILRTDPAVDYINSTVGAGGPNATANNGRLFVALKDRKE
                     RDTAPVVIARLRQKATLVPGMQAFFQSVQNLNIGGRPSKSQYQYTLQSGDTESLYRLA
                     PEMRDRIAQVPGLLDVTTDLYVKNPQMTVDIDREKAAVYGITVDQVRNQLYNAYGARQ
                     VGTIYMPSNDYQIILEAQPQFRVDPSDLSKLYMKTANNQTIPLDAVAKLVPTVGPLLI
                     NHQGQQPAVTISFNLAPNYSLGYAVDQITSIEQSSNLPATIATGFAGTAQVFQDSLRG
                     QGVLILAAVFAAFVILGILYESFIHPITIISGLPSAGIGAILTLMLFNMELSVIAMIG
                     IVMLVGIVKKNAIMMVDFALERRRVGLSAEHAIREAALLRFRPIMMTTFAAIFGTLPI
                     AIGAGAGAELRQPLGIAVVGGLCVSQLLTLFITPVIYIYLDRIDRRLKRRLEPQADEF
                     DDVERPKVAAAE"
     gene            5933..6496
                     /locus_tag="VGA15_19390"
     CDS             5933..6496
                     /locus_tag="VGA15_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX9589896.1"
                     /translation="MNSYFRRQLADYVEYHRDPWNCAMHVFGIISLFLAAVLPLNFWS
                     VQIFGVQTTMAAMMVLPVLIYWLLLDTALGMGIVGAAVLLLSTAAIIVSNVSVAAVWS
                     ISAVLMVVGVGMQIIGHRLFERRQPALLDNPPHLLLGPMFVMAKLFIALGFRHDLALI
                     IDPVPQTAALGTTFYADARNGETHPNS"
     gene            6493..7545
                     /locus_tag="VGA15_19395"
     CDS             6493..7545
                     /locus_tag="VGA15_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX9589897.1"
                     /translation="MTSVLVTGGSGFIGQHLVSTLVDRGLQVRVLDIHPPTHPEPEVE
                     YVEGSVLDPDRVGEAVRGVEQVYHLAGLPGMWMRQKSDFHAVNCRGTEVVLAAARKHG
                     TGRFLHCSTESILFRSSASEEAGADYTLPMADHMPGPYTRSKMLAEQRAMQAAASGFP
                     VVIGTPTMPIGLHDHNLTPPVAMLRHFLNRRFQLYLDFIVNLVDVRDVAAGLLLAMER
                     GQVGHRYIFGGESIPLKEVLRLVAENNDRRSFLIPVPAKVAEAAAAMMELIADYVTRK
                     PPSGTAEGVRIARRATSLSIEKAERELGYAPRPVEPALRETIAYLRDAAGDQQRSGRA
                     GVSRVRPAHAGRRYGS"
     gene            7572..8198
                     /locus_tag="VGA15_19400"
     CDS             7572..8198
                     /locus_tag="VGA15_19400"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9589898.1"
                     /translation="MSYDPRELLAVVWSGWTATWPTQLLAIIWITWLISWVVASFWSG
                     RTQKHVMTWDSQAYRIPILVGGILFTPWTAQILGEKPLWQFGNPGIYVLSVLTLAGIS
                     FTWWARIHLGRFWSNSITRKEGHRVIDTGPYGLVRHPIYTGLIAGMLATGIAVGTVTA
                     MLGAVLISLGMWQKARMEEGFLTNELGADAYGSYCRRIPMLIPFLPPR"
     gene            complement(8221..9168)
                     /locus_tag="VGA15_19405"
     CDS             complement(8221..9168)
                     /locus_tag="VGA15_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX9589899.1"
                     /translation="MNSPAPVATFMIGAMLSAGVLCGSFDTVLAGQIAVSSNAAAEAV
                     ALPPATNPPATEPHGRAYLFRGALGPIFSRGMDRLTEKIEHAGVTANVYEFTICRLIA
                     DSAIREYRQDPAPIILIGHSMGGYCALIFAEMLQTENIPVSLIVTVDPAHVTPNVPLN
                     VERYINIFLSKSVLGGGDVKPAQGYQGDYASFDLSKQEDVTHINIDKMDLIHQQIVSK
                     IVELGTTPAKAEGEAVPLRYVVPPNTAIELWDSGTLVVARPGDTLQKLATLRHLPLWS
                     LTQINRESDSVPLVPGQRVIVPRHLVPFAEVSAQPPSRR"
     gene            complement(9447..10199)
                     /locus_tag="VGA15_19410"
     CDS             complement(9447..10199)
                     /locus_tag="VGA15_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589900.1"
                     /translation="MKPLNGYKGFVDGLSVLRGPWLRPLRAAAMTVLLLVVPVGTGTA
                     NATATAAPATGAHVYLLRGVLNIFSLGLDDIAAKLERQGIPVTVANFVSWSSLADDAA
                     AEYRSGKVRTIILVGHSSGATALPDMVAKLGQLGVPVKLAIGLDSVFRTKLAGHAERY
                     INFYVASGAGEPVTRTRDFRGTLENVDVQAVPGVGHLSIDKNQIMQQKVINAIDAAVR
                     HAATAAVAQTPQQREFSAARQSPGTSAAAARY"
     gene            complement(10431..10760)
                     /gene="erpA"
                     /locus_tag="VGA15_19415"
     CDS             complement(10431..10760)
                     /gene="erpA"
                     /locus_tag="VGA15_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX9589901.1"
                     /translation="MTTAVTISERAARRIGEILKTEGDGAMLRISVEGGGCSGFQYKF
                     DVERAQAEDDLVIARDSAVVLVDPASVPFLAGSEVDFVDDLIGASFRVVNPNATASCG
                     CGTSFSI"
     gene            10871..12079
                     /locus_tag="VGA15_19420"
     CDS             10871..12079
                     /locus_tag="VGA15_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX9589902.1"
                     /translation="MSVGMAAPRSVFACDPDHSRGRLFVEPPSKTRSPFRRDCDRVIH
                     STAFRRLKYKTQVFVFHEGDHYRTRLTHSLEVAQIARALARQLGLDEDLTEVLALAHD
                     LGHPPFGHAGERALNACLQAHGGFDHNAQTLRVVTSLEHRYPDFDGLNLTWETLEGIV
                     KHNGPLMERSGAPVGRYREHGIPVGISEYDRTHDLELWSFASPEAQVAAIADDIAYDA
                     HDIDDGLRAGLFTVDDLKIIPLAAEMIADIARHYPGLDDDRRGAELVRELISRLIGAV
                     VSEARRRLEAAQPKSVDDVRGHGEVLIAFSTDVGEAEAGIKSFLKLRMYRHSRVMRVM
                     GDAEQILFDLFARYQKLPDDLPPEWRPPDGRDSETDRARRIGNFIAGMTDRFALTEHQ
                     RLFDSTPDLR"
     gene            12112..14319
                     /gene="argS"
                     /locus_tag="VGA15_19425"
     CDS             12112..14319
                     /gene="argS"
                     /locus_tag="VGA15_19425"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX9589903.1"
                     /translation="MAKKTSKKAKQAAKKSAKKSAKKKLAEKKLATTKPVKKPVKKPV
                     KKSTKKTAKKATPKANPARGAAKRAAKGRAKKARAKSRVSPTSGKTAIAPVAEDKASA
                     EAIRATEKPVKESTKPLPKQTGHGAINEPPAHPIPAAKAAPEQHLFANLLARVHAVCR
                     TLGEESGWYADVDLSRVVVEPPRDAAHGDMATNAAMVLARDAKAKPRDLAEQIAAKLR
                     ADDLIASVDVAGPGFINLTLKASVWSDALRSVLREGDSYGRSGIGAAEKVDVEYVSAN
                     PTGPMHVGHCRGAVFGDALCSLLQFAGYAVTREYYINDAGAQVDVLARSAFLRYREAL
                     GEDIGEIPEGLYPGDYLKPVGQALAAEYGDRLLAMPEGGWLPAVRAKAIAMMMEGIKG
                     DLAALGIKHDVFFSERSLIETGNNRVTATIDYLRSKGDVYEGRLPPPKGGAPVDDYED
                     REQTLFRATAYGDDVDRPLMKSDGSYTYFASDIANHKNKFDRGFRNLIDVFGADHGGY
                     IKRMQAAVKAVTSGEATLDVKVVQLVRLLRHGEPVKMSKRSGEFVTLREVVDEVGSDA
                     VRFMMLFRKNDAVLDFDLAKVIEQSKDNAVFYVQYGHARGHSVFRNAREVVPDLPEEA
                     SARAAFLGDAAMERLTDPGEIDLLRRLAAYPRMIEAAAVAHEPHRIAFYLYDLASEFH
                     ALWTRGRDLPYLRFIINNDADITRARLALVQGVVSVLASGLAVLGVDAPAEMR"
     gene            complement(14382..14825)
                     /locus_tag="VGA15_19430"
     CDS             complement(14382..14825)
                     /locus_tag="VGA15_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589904.1"
                     /translation="MQWMDGARVFRRVLHRREFLPLRDFLPLRDFLPLRDFLPLRDFL
                     PGGALHPLRRPCGGGLICGHRALVLIALTATRRYLLIGPAHAGERIGLTDQPREFGQR
                     IAFSPSRRKLVTATIMIVARWKRFILISISHRDDACPSGKPPTHF"
     gene            14781..15926
                     /locus_tag="VGA15_19435"
     CDS             14781..15926
                     /locus_tag="VGA15_19435"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX9589905.1"
                     /translation="MEYPPEHPSAVHPLHRHAPPRATLEPRYHEAPPFADDDQEPEPD
                     PSRYDEALYGQLDHGAEEPQYDQAYAEEDDPYGYQDGYGDGDGTEERVPKRSRGIMTI
                     AIVLALAVVGTGAAFAYRTFVGASRSGEPPIIRADAGPTKIVPSPADATTKVPDRMVA
                     GDGTERIVPREEAPVDVNAKSGPRVVFPPLNPNPNPPAASSVASNALPPASGGNGTLA
                     SNEPRKIRTLSVRGDQPDAAATPVGAAPPAKPTPATRSVANANASTNAPLALTPQQSA
                     VPVVEPRTRVAASTPTDQAAPTGGGYLVQVSSQRNEADAQASFKALQGKFPAVLGSKD
                     PVIKRADLGEKGVYYRAMVGPFGSSDEASQFCGSLKTAGGQCVVQKN"
     gene            16021..17049
                     /gene="nagZ"
                     /locus_tag="VGA15_19440"
     CDS             16021..17049
                     /gene="nagZ"
                     /locus_tag="VGA15_19440"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX9589906.1"
                     /translation="MSSRAFITGVSGPELTAAEREFIAAERPWGFILFKRNIETPAQV
                     ILLVRELRAAIGNPDAPVLIDQEGGRVQRLGPPHWPVYPPGAAFGVLYDIDRAAGLTA
                     ARLSARLMAADLIELGITVDCLPLADVPVAGADAVIGNRAYGTEPDKVAAIARAVTEG
                     LEQGGILPVLKHIPGHGRATADTHFRLPEVDTSKAELERTDFAAFQPLADLPMAMTAH
                     VVFSALDPAQPATTSATIIQQVIRGGIGFQGLLMSDDVSMNALAGSIAERTRAIIAAG
                     CDMVLHCNGKLDEMREVARETPELSGKALERAGRALASRRAPQAFDRLAGRAELDALI
                     DRAGAKGA"
     gene            17046..17873
                     /locus_tag="VGA15_19445"
     CDS             17046..17873
                     /locus_tag="VGA15_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEX9589907.1"
                     /translation="MSAEILSFETGRPAELAENEPALVVDVEGYEGPLDLLLALARQQ
                     KVDLAKISILALADQYLVFIEAARKIRLELAADYLVMAAWLAYLKSRLLLPEPATADG
                     PSAEEMATALANRLRRLEAIREASNRLMNRPQLQRDIFPRGDPESIAEIKHPKFTATL
                     FDLLTAYAAQRQQRVLASVHLTKRTVWSLSEARASLERLVGLAEDWSRLDQYLLAYIV
                     DPSQKATVFASSFAAALELVREGAVELHQKQAFAPLYFRKRPPQPGSDAITAPDLPE"
     gene            17909..18625
                     /gene="scpB"
                     /locus_tag="VGA15_19450"
     CDS             17909..18625
                     /gene="scpB"
                     /locus_tag="VGA15_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX9589908.1"
                     /translation="MASLAEKRNADAEEDSVDPQARPEELRLLEALLFASSEPLDQAA
                     LAKRMPDGVDVKVALAQLQADYASRGVNLVRVANKWTFRTAGDLSWLMTRETTETRRL
                     SRAAIEMLAIIAYHQPITRAEIEDIRGVITSKGTLDVLLETGWIRPRGRRKTPGRPLT
                     FGTTEAFLSQFSLEALGDLPGLEELKGTGLLDSRLPTGFSVPSPSDDPALRDDEDPLE
                     IGDLELALAPAVEPESEDGS"
     gene            18793..>18911
                     /locus_tag="VGA15_19455"
     CDS             18793..>18911
                     /locus_tag="VGA15_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX9589909.1"
                     /translation="MGSLSIWHWIVVIAVVLLLFGRGKISDLMGDVAQGIKAF"
BASE COUNT         3596 a         5795 c         5924 g         3596 t
ORIGIN      
        1 gcgggccagg gagatgttct cctatgccgc atcgtatttc ggcaatgccg acgcgcagta
       61 tgatctggcc cggctctatc tgcggggcct cagctcgtcg cgggaggact tccgctatgg
      121 cgcccgctgg ctcggccttg ccgcccagaa gggccagcat caggctcagg ccatgctcgg
      181 tcagatgctg ttcaacggcg accagttgcc gcgtcaggcc gcccgcgggc tgatgtggct
      241 gacgctggcg cgcgacagcg ctacgccgga cgaaacctgg atcaaggaaa gctacaacaa
      301 ggcgatcgcc aaggcttccg acgacgaccg cgcgatggca ctgcaaatgc tcgagcactg
      361 ggtgcagggc cgcaaggatt gaggctctga tgcaggcagc cgtcaccctg aggtggcgga
      421 gcgctgcgcg tgttacgccg tctccaggtc gagatcgatc cagaccggca cgtgatccga
      481 cggtttttcc caggcgcgga cataactatc gatcccgaca tcgatcagcc ggtcgctggc
      541 ctgcggcgac agcaagagat gatcgatgcg aagcccatgg tttttctgcc aggcgccggc
      601 ctgatagtcc cagaaagtgt attggcccga cccgtcggtg acggcgcgca gcgcatctgt
      661 gagcccgagg ccaagcagcg attgaaagct ttcgcgggtg gcgggacgga acagcgcgtc
      721 gttgacccag gcggccggat tatggacgtc ctgcgccgcc ggaatgacgt tgaagtcgcc
      781 ggcaagcacc agcggctcct cggtcttcga ccgttgcttc gaatactcat gaagccgtga
      841 catccatctg agcttatagg gatatttatc ggtctcgggt gggttgccgt tgggcagata
      901 gaggcacgcg atccgcagca cgccatgctt cagcgtgacg acaccttcca gaaagcgcgc
      961 gtggatgtct tcatcatcgc cggcgaggcg cggcctggtt tcgtcgaacg gcagcttcga
     1021 cagcaacgcg acaccgttga aggtcttctg gccgtgggtg accacgttgt agccgagcgc
     1081 ctcgatcgct tcgcgcggaa acgcctcatc gacgcatttg atttcctgca gacagacaat
     1141 atcgggcgta caatcctgca gccaggtcag caggtggtcg agccgctgcc tgatcgaatt
     1201 gacgttccac gtagcaatac gcatgaaaaa gccccggaca agtaagcaca aattttcgat
     1261 tctgcgacat accatgcgcg gcggggctgt gataaccctt tacaaataag gcgcaaatgt
     1321 cgccgttata gggcgggaaa ccggatcgtc ccggtcgttg cggaccggct ggcgtgaaat
     1381 gcgatgaaaa agcgtaactg gatcatttta gccggtgggc taggcatcat tgctctggcg
     1441 gccttcgcga cgcgcgcgtc ctggatgggc ggcggagcga gcgcccaggg accacagcgc
     1501 gcgcgcgtga tttccgttga actggccaag gcggagcgca agcccgtgcc ggtcgatgtc
     1561 gattcaatcg gaacggtgac gcccatttcc agcgtggcgc tgaaatcgcg ggttgaaacc
     1621 accatcgtct cggtccattt cgaagacggc gcgaaggtca gtgaaggcga tctgctgttt
     1681 accctcgatg cacgccagat cgacgcccag atcgaacagg ccgagggcat gctcgccaag
     1741 gatcaggccc agctcgaggg cgcgcaacgc gatctccgcc gcttcagcga cctgatcggc
     1801 aagggcgcca ccacgcaggt caatgtcgac aacgccaaaa cccaggccga tatcctgatc
     1861 ggtacgatca aggccgatca gtccgcactg gacaatctga aggtccagaa aagcttcacc
     1921 atcatccgcg caccgttttc gggacggatc agcgcggcga acgtgaaaat cggcaatttc
     1981 gtgcgcccgg ccgacaccgc gccgctcgcc gtcatcaatc aaatggcgcc ggtctatgtg
     2041 accttcgcca ttccgcagcg tgtcctggtc gacctgcgcg atgccatggc gaccggcgac
     2101 tccagggtga ttgcgacgat ccctggccac gagcgctccg agggcggcaa ggtggcgatg
     2161 gtcgaaaatt cggttgattc caccaccggc atgatcaccg tgcgcggcat catgaacaat
     2221 gcgaacgaaa ccttatggcc ggggattctg gtgaacacga agctcatcat ccggaccgag
     2281 gaagcggtga cggtgccctc ggccgccgtg cagcgcagcc agaacggcaa tttcgtcttc
     2341 atcatgcggg acggcaaggc gcacgtggaa ccggtaagtg tcagccgaac gtctcaggga
     2401 atgtccgtca tcgccagcgg attgtccggc ggcgaggacg tcattgtcga tgggcagttg
     2461 ctgttgtccg aaggaactcc ggtcgcgccg cgcgcaccta aggcgggggc ctgatcgatg
     2521 accctttcgg agctgtgtat ccgccgcccg gtcatgacga ccctgatgac ggcttcgatc
     2581 atcgcgttcg gtgtgttcgg cttccggctc ctgccggttt ccgcgctgcc caaggtggat
     2641 ttcccgacca tcgcggtgac cgcgaccttg ccgggcgcca gcgccgacac catggcggcg
     2701 tccgtcgccg gtccgatcga acgtcagctc tcgacgattg ccggcatctc gtcgatgtcc
     2761 tccagttcgt cacagggcct gagcgtcatc accatccagt tcgacctcaa tcggaacatc
     2821 gatggtgcag cgctcgacgt gcagaccgct ttgacaatcg cgcagcgccg tctgccgatc
     2881 gagatgacca ttccgccgag ttttcgcaaa gtgaatccgg ctgatttccc ggttctgttc
     2941 gttacgcttg ggtccacgac gctgccgctg tcggctgtca atgaatacgg cgacattacc
     3001 gtcgggcagg cgctgtcgca gctcccgggc gtagcgcagg tcaccatttt cggcgcgcag
     3061 aagttcgcaa tccgggtaca ggccgatccc gaagcagccg cggcgcgcgg gctctcgctc
     3121 gaagatatcc gcgctgccgt ttctcgcgcc aactcctcga cacccgtggg cacgctgaac
     3181 ggcccgaagc aggacattgc ccttgtcgct tccgggcaga tggacagggc cgccgactat
     3241 cgccacatcg tcgtcgcatg gcgtaacggc tcaccggtca agctggacga ggttgcacac
     3301 atctacgaca gcgtggaaaa cgacaagatc gcgacctggt tgaatgaccg tcgggcgatc
     3361 gtgctggcca ttcaaaaaca gccggatgca aacaccgtcg cggtggttga tggtgtgcgg
     3421 gccaagctgc cttcactgcg ggcgcaaatt ccaccatcgg tggtgatgga agtcctgctc
     3481 gatcgatcgg tttcgatccg acaatccgtg agcgatgtcg aggaaaccct gctgatcgcc
     3541 gtagcattgg tgatcatggt gatcttcctg ttcctgcgct cggcatcggc cacgttcatt
     3601 cctgcgctcg cggttccgat ttcgctgctc ggtacctgcg cggtcatgta catgctggat
     3661 tactccatca acaacatgac gctgctggcg ctgacgctgt cggtcggctt cgtggtcgac
     3721 gacgccatcg tcatgctgga aaacatcgtc cgccatatcg aacacggcac gaagccatat
     3781 gaggcggccc tcaagggcgc gcgcgaaatc ggctttacca tcatttcgat caccttctcg
     3841 ctgatcgccg tgttcattcc ggtgttgctg atgggcggca tcgtcgggcg cgtgttccgc
     3901 gaattcgcgg taacgatcgc ggtcgcgatc gtggtttccg gttttgtatc gctgacgctg
     3961 acgccgatgc tgtgcgcgcg ggtgctgaag gcgcacgacg aaaccaggag gccgaatatc
     4021 gtccttcgcg tttttgagcg gattttcgat tcctggctcc ggggctacga atgggcgctc
     4081 gatcgggtgc tcgcccgcaa ggcaatgatg ctggtggtga cgtttgccac gctcgccggc
     4141 accgtcgcgc tctatgtcgt cgtgcccaaa ggctttttcc cgcaggagga tacaggcttt
     4201 ctgttcggga cgaccgaagc agcgaccgac acgtcgttcg aggcgatgaa ggatcggcaa
     4261 ggggcattgg tcgcgatttt gcgaaccgat cccgcggttg attatatcaa cagcaccgtc
     4321 ggcgcgggcg gcccgaacgc gaccgccaac aacggaaggc tattcgtcgc gcttaaagat
     4381 cggaaagagc gcgacactgc cccggtcgtg attgcccggt tacgtcagaa ggccacgcta
     4441 gttccgggaa tgcaggcgtt tttccagagc gtgcaaaacc tcaacattgg cggccggccg
     4501 tcgaagagcc agtaccaata tactttgcag agcggcgaca ctgaatcgct ctatcggctg
     4561 gcgcctgaaa tgcgcgacag gatcgcgcaa gtgccgggac tgctcgacgt caccaccgat
     4621 ctctatgtca agaatccgca gatgacggtc gatatcgatc gggagaaagc cgcggtctac
     4681 ggcatcaccg tcgatcaggt gcgcaaccag ctttataacg cgtatggcgc ccgtcaggtc
     4741 ggcaccatct acatgccgtc gaacgactac cagatcatcc tcgaagcgca gccgcagttt
     4801 cgggtcgatc cgtcggatct ttccaaactc tacatgaaga ccgcgaataa tcagaccatc
     4861 cccctggatg cggtggccaa actggtaccg acggtgggac cgctgctaat caaccaccag
     4921 ggtcagcagc cggcggtgac gatctcgttc aacctcgcac cgaactactc gctgggttat
     4981 gcggtggacc agatcacgtc gatcgagcaa agctcaaatc ttcccgcgac catcgccacc
     5041 gggttcgccg gcaccgcgca ggtgttccag gattcgttgc gcgggcaggg cgtgctgatc
     5101 ctggcggcgg tgtttgcagc cttcgtgatc ctcggcattc tctatgagag tttcattcac
     5161 cccattacga tcatttccgg cctgccgtcg gcgggaatcg gtgcgatcct cacgctgatg
     5221 ctgttcaata tggaactctc ggtgattgcg atgatcggca tcgtgatgct ggtcggcatt
     5281 gtcaagaaga acgccatcat gatggtcgac ttcgcgctgg agcgccgtcg cgtgggcttg
     5341 agcgccgaac atgctattcg cgaagccgcg ctgctgcggt tccggcccat tatgatgacg
     5401 acgttcgcgg cgattttcgg cacgctgccg attgcgatcg gcgccggcgc tggtgctgag
     5461 ctgcgtcagc cgctcggcat cgccgtggtc ggcggtctct gcgtatcgca gcttttgacg
     5521 ctgttcatca cgcctgtgat ctatatctat ctcgaccgga tcgaccgccg cctgaagcgc
     5581 cgcctcgaac cgcaagcaga cgaattcgac gatgtggagc ggccaaaggt cgcggcggcg
     5641 gaatgacaga tcgatgatgc agggtcctgc gccgtcttga tcaaaacgcc ggatgctatg
     5701 gataagtcat tgagcgcgaa tctgattttg cgcgcgcaac ggtggtacgg cgctacttgt
     5761 cgtcctcttg tccgcgatct gcaaacaaca aatagcgcag ccgcgctttg acttgggcct
     5821 agtcccacgt ctaaggtacc tgccagggag ctaggtgatg aagggggacg cctaattctt
     5881 gtgaccgccg gccgcggaag ttcccgcagg cgagacatgt agtgaactca gtatgaattc
     5941 ttattttcgg cgccagcttg cggattacgt cgagtatcac cgcgatccct ggaattgcgc
     6001 tatgcacgta ttcgggatca tatccttgtt cctggccgct gttctccccc tcaatttctg
     6061 gtcggtccaa atatttggcg tccaaaccac gatggctgcc atgatggtct tgcccgttct
     6121 gatttactgg ttgctgctcg ataccgctct cgggatgggg atcgttggcg ccgcggtttt
     6181 attgctttcg actgcggcca tcatcgtcag caacgtaagt gtcgccgccg tgtggtcgat
     6241 ctcggctgtt ctgatggtcg tgggcgtcgg tatgcagatt atcgggcatc ggctgttcga
     6301 acgccgacag ccggcgcttc tggacaatcc gccccatctg ttgctcggtc cgatgtttgt
     6361 gatggcgaag ctattcattg cgctcggctt taggcacgat cttgccctga tcatcgatcc
     6421 agttccgcaa acagcggctc tgggcactac gttttacgcc gatgcacgta acggcgagac
     6481 gcacccaaac tcatgacctc tgtactcgtt accggcggca gcggattcat cggacagcac
     6541 ttggtatcga cgctggtcga ccggggtctg caggtgcgcg tgctggatat ccaccctccg
     6601 acccatcccg agccggaagt cgaatatgtc gaaggctcgg tgctcgatcc ggaccgggta
     6661 ggggaagccg tgcgcggcgt cgaacaagtc tatcatctcg ccggtctgcc cggcatgtgg
     6721 atgcggcaga aaagcgattt tcatgcggtc aattgccgcg gcacggaggt cgtccttgct
     6781 gctgctcgca agcatggcac cgggcggttc ctgcactgct cgactgaatc cattcttttc
     6841 cgctcttcgg cttccgagga agccggcgcg gattataccc tgccgatggc tgaccacatg
     6901 ccggggcctt atacccgctc caagatgctg gcggagcagc gcgcgatgca ggccgcggcg
     6961 tccggttttc cggtcgtcat cggcacgccg accatgccga tcgggcttca cgatcacaac
     7021 ctaactccgc ctgtcgccat gctcaggcat tttctcaacc ggcgttttca actgtatctc
     7081 gatttcatcg tgaaccttgt cgacgtgcgc gacgtcgcag ccggtctgtt actggccatg
     7141 gagcgcgggc aagtcgggca tcgctacatt ttcggcggcg aaagcatccc gctgaaggag
     7201 gttcttcgcc ttgtggcaga aaataacgac cgccgtagtt ttcttattcc ggtccccgcc
     7261 aaggtcgcgg aagccgccgc cgcaatgatg gaactcatcg ccgactatgt gacgcgaaaa
     7321 cctccttccg gaacggcgga aggtgtgcgg atcgcgcggc gcgcgacgtc cctttcgatc
     7381 gaaaaggccg agcgcgaact tggctacgcg ccgcggcctg ttgagcctgc gttgcgcgag
     7441 accatcgcct atctccgtga cgccgccggc gatcagcaac gatccggtcg cgccggggtt
     7501 tcgcgtgttc ggcccgctca tgcgggtcgg cggtatggct cataacgcga tcctgtcata
     7561 taatctgaac catgtcttat gatccgcgcg aattacttgc cgtcgtctgg agtggatgga
     7621 ccgccacttg gcccacccag ttgctggcga tcatctggat tacctggctt atcagctggg
     7681 tcgtggcgtc gttctggtcg ggtcgcacgc aaaaacacgt gatgacctgg gactcgcaag
     7741 cctaccgtat tcccattctg gtgggaggca ttctctttac gccatggacc gcgcagatcc
     7801 tgggcgaaaa gccgctctgg caatttggca atcccggcat ctacgtgctt agcgtcctga
     7861 ccctggcggg gatctcgttc acgtggtggg cgcggattca ccttggacgc ttctggtcga
     7921 actcgatcac gcgcaaggag ggtcatcggg tcattgacac cggcccgtat ggacttgtgc
     7981 gccacccgat ctacacgggc ctcatcgccg gcatgttggc gaccggtatc gccgtcggga
     8041 cggtgaccgc gatgcttggc gccgtgctga tctccctcgg catgtggcag aaggcacgca
     8101 tggaagaagg cttcctcacc aacgagctcg gcgcggacgc ctacggatct tactgccggc
     8161 gtattccgat gctgattcca ttcctgccgc cgcgctaacc cgtcattgac gggccgcacg
     8221 tcagcgcctg gacggtggct gcgcggagac ctcagcgaac ggcacaagat ggcgtggaac
     8281 aatgacgcgt tgaccgggca ccagcggtac gctgtcggac tcccggttga tctgggtaag
     8341 cgaccagagc ggcagatggc gaagtgtcgc gagcttctgc agcgtatcgc ccggacgtgc
     8401 gactaccagc gtaccgctgt cccacagctc gatggcggtg ttgggaggaa cgacatagcg
     8461 aagcggcacg gcctcgcctt cggcttttgc cggggtcgtg ccaagctcta cgatcttgct
     8521 gacgatttgc tggtggatca ggtccatctt gtcgatattg atgtgcgtca cgtcctcctg
     8581 cttcgacagg tcgaagctgg catagtcgcc ctgatagcct tgtgccggct tgacgtcgcc
     8641 gccgcccaga acgcttttcg acaggaagat attgatgtag cgctcgacat tgagcggcac
     8701 gttgggcgtg acgtgagctg gatcgaccgt gacaatcagg cttaccggga tgttttcggt
     8761 ctgcagcatt tcggcgaata tcagcgcaca atatccgccc atcgagtgcc cgatcaggat
     8821 gatcggagcg ggatcctggc gatactcgcg aatcgccgaa tcggcgatca gacggcagat
     8881 cgtgaattcg tagacgttcg ccgtgacacc ggcgtgttcg atcttttcgg tcagacggtc
     8941 catcccgcgc gaaaagatcg gaccaagagc gccacggaac aggtaggcgc gcccgtgcgg
     9001 ctctgtcgcc ggcggattgg tggcaggcgg gagtgcaacc gcttcagcgg cggcgttgct
     9061 cgacaccgcg atctggccgg ccaacaccgt atcgaagctg ccacatagga cgcctgccga
     9121 aagcatcgcc ccgatcatga atgttgcaac tggcgccgga ctgttcatgc gcttcctgct
     9181 tgtaccttcg acgaacaggc acgcgaaaca ccttagacgc tggcggatca atcggacaga
     9241 taaaaccccg ccaacgtcct tccgcctgaa gccgcggaat tcgtcacgga ctcatccgtc
     9301 tgattttcgc ccttacgcac agccgccgat gtcagcaaag cccatcgccg aaagtgcgcc
     9361 atccgccgca cgatggcctg tatctccgac gaattggcag aatttgaggc gcgctttggg
     9421 ccggacgcgc ggtccggagc ttgagttcag tagcgcgcgg cagcggcgct cgtacccggg
     9481 ctctgcctcg ccgcgctgaa ttcccgctgc tgcggtgtct gcgccacagc cgccgttgcc
     9541 gcgtgcctga ccgcagcgtc gatggcgttg atcaccttct gctgcatgat ctggttcttg
     9601 tcgatcgaga ggtgcccgac accgggcacc gcctgcacgt cgacattttc cagcgtgccg
     9661 cgaaaatccc tggtcctcgt cacgggctcg ccggctccgc tggcgacgta gaaattgatg
     9721 tagcgctcgg cgtggcccgc cagcttggtg cggaacaccg agtcgaggcc tatcgccagc
     9781 ttgaccggga ccccaagctg accaagcttt gccaccatat ccggcagcgc cgttgcgccc
     9841 gaagagtgcc cgaccagaat gatggtccgc acctttccac ttctgtattc cgccgcagca
     9901 tcgtctgcga gcgacgacca cgagacaaaa ttggcgacgg tgaccgggat gccctgccgc
     9961 tccagcttcg ccgcgatatc atccagaccc agcgagaaga tattaagcac gccgcggagg
    10021 agatagacat gagcgcctgt cgcgggagca gcggtggccg tcgcgttcgc cgtgccggtt
    10081 ccgactggaa cgaccagcag cagaaccgtc atcgctgccg ctcgcagagg acgcaaccac
    10141 ggcccgcgca ggacggaaag tccgtcaaca aatcccttgt agccgttcaa tggcttcatt
    10201 ggtcctcctc gcaggcgtac cgaatcaggc gtaccgaata ttgtgcacga tattgtgcaa
    10261 agcaacgtag ccttcgcagg ctcgcaactg caacagggcg aggtcataag agccattttt
    10321 atccggagtg cgtgtcaggc ttgcaacagc atcggcaaaa aattccccat ttgtgcggac
    10381 atggagttta atcaggcgaa gtgctgccga cgtcgtctga tttaaggttc tcagatcgaa
    10441 aaactggtgc cgcagccgca ggacgcggtg gcattcgggt tgacgacccg gaacgaggcg
    10501 ccgatcagat cgtcgacgaa atcgacttcg gaacccgcca gaaacggcac cgaggccgga
    10561 tcgaccagca ccaccgcact gtcgcgcgcg atgacgaggt catcttcagc ctgcgcgcgc
    10621 tcgacgtcga acttatattg aaagcccgag cagccgccgc cctcgacgct gattcggagc
    10681 atggcgccgt cgccctcggt cttgaggatt tctccaatgc ggcgggcggc ccgctcgctg
    10741 atggtgacgg ctgtagtcat gtgagaggtc tccaatcgcc gatgccatcg ccaaattatt
    10801 tggaatccgg cctccagcat agttaagtgc gccagacgct ggaatcaaac cttttgggga
    10861 acaatacacc gtgtcggtcg gaatggctgc tcctcgctcg gtctttgcct gcgaccccga
    10921 ccacagccgc gggcggctgt tcgtcgagcc gccgagcaag acccgtagcc cgttccggcg
    10981 cgattgcgac cgggtgatcc actccaccgc ctttcgccgc ctgaagtaca aaacccaggt
    11041 cttcgtgttc cacgagggcg atcattaccg cacccggctg acccacagcc tcgaagtggc
    11101 gcagatcgcc cgcgcgctgg cccggcagct cggtctcgac gaggatctga cggaagtgtt
    11161 ggcgctggcc catgacctcg gccatccgcc gttcggccat gccggcgagc gcgccctcaa
    11221 cgcctgtctg caagcccatg gcggattcga tcacaacgcg cagaccctgc gcgtcgtcac
    11281 ctcgctggaa catcgctatc ccgatttcga tggcctgaac ctgacctggg agacgctgga
    11341 gggcatcgtc aagcacaatg ggccgttgat ggagcgaagc ggagcgccgg tcgggcgcta
    11401 ccgcgagcac ggcattcccg taggcatttc cgaatacgac cggacccacg atctggaact
    11461 gtggagcttt gcctcgccgg aggcccaggt cgccgcgatc gccgacgaca tcgcctatga
    11521 cgcccacgac atcgacgacg ggctgcgggc cgggctgttt acggtcgacg atctcaagat
    11581 catcccgctc gccgccgaga tgatcgccga tatcgccagg cattatcccg gtcttgacga
    11641 cgaccggcgc ggcgccgagc tggtgcgcga gctgatttcg cgtttgatcg gagcggtcgt
    11701 ctcggaagcg cgcaggcgtc tggaagcagc acagccaaaa tccgtcgatg atgtccgcgg
    11761 ccatggcgag gtgctgatcg cgttttctac tgacgtaggg gaagccgagg ccggcatcaa
    11821 gtcgttcctg aaactaagga tgtaccgcca ttcgcgggtg atgcgggtga tgggcgatgc
    11881 cgaacagatc ctgttcgact tgttcgcgcg ctatcagaag ctaccggacg atctgccgcc
    11941 cgaatggcgg ccgccggatg gcagagacag cgaaaccgat cgcgcaaggc ggatcggcaa
    12001 cttcatcgcc ggaatgaccg accgcttcgc cctgaccgaa catcaaaggc tttttgactc
    12061 gacccccgat ttgcgttagg cggcgagcca cctccgcaaa agcaggcaat aatggcgaag
    12121 aaaacttcaa agaaggcgaa gcaggcggcg aagaaatccg ccaagaaatc cgccaagaaa
    12181 aaactcgccg aaaagaaact cgccacaaca aaaccggtca agaagccggt caagaaacca
    12241 gtgaagaagt ccacgaaaaa aactgcgaaa aaagccactc caaaggcaaa ccctgccagg
    12301 ggcgcggcga agcgtgctgc caaaggccgc gccaaaaagg cgcgtgcgaa atcccgggtt
    12361 tctccgacat ccggcaaaac cgcgatcgcg cctgtggccg aggacaaagc atccgctgaa
    12421 gcgatcaggg caactgaaaa accggtcaag gaaagcacca agcctttgcc gaagcaaacc
    12481 gggcacggcg caatcaacga acctcccgct cacccgatac ctgccgccaa agctgcgccc
    12541 gagcagcatc ttttcgccaa cctgctggca cgcgttcatg cggtttgccg cacactcggc
    12601 gaggaaagcg gatggtatgc agacgttgac ctgtcgcgcg tcgtggtcga accgccgcgc
    12661 gatgctgcgc acggcgacat ggcgaccaac gccgcaatgg tgctggccag ggatgcgaag
    12721 gcaaaaccgc gcgacctggc cgagcagatc gcagccaagt tgcgcgcgga cgatttgatt
    12781 gcgtccgtcg atgtcgccgg tcccggcttc atcaatctca ccttgaaggc gtcggtctgg
    12841 tcggacgcgt tgcgctccgt gctgcgtgag ggcgatagct acgggcgaag cgggataggt
    12901 gcggccgaga aagtcgatgt cgagtacgtt tcggccaatc cgaccgggcc gatgcatgtc
    12961 ggccattgcc gcggggcggt gttcggcgat gcgctgtgca gcctgcttca gttcgcgggc
    13021 tatgcggtca cccgcgaata ttacatcaac gatgccggcg cccaggtcga cgtgctcgcg
    13081 cgttcggctt tcctgcgcta tcgcgaggcc ctcggcgagg acatcggcga aattccggaa
    13141 gggctctatc ccggcgatta tttaaagccg gtggggcagg cgctggccgc tgaatatggt
    13201 gacagactac tcgcgatgcc ggaaggcggc tggctgccgg cggtgcgggc caaggccatt
    13261 gcgatgatga tggaagggat caagggcgat ctcgccgcgc tcggtattaa gcacgacgtg
    13321 tttttttcgg agcggtcgct gatcgaaacc ggaaacaacc gggtcaccgc aaccatcgat
    13381 tatcttcgtt ccaaaggcga tgtttatgaa ggccgtctgc cacctccaaa aggaggcgcg
    13441 ccggtcgatg attatgagga tcgcgagcag acgctgtttc gtgccaccgc ctatggggac
    13501 gatgtcgatc gtccgctgat gaaatcggac ggcagctaca cctattttgc ttctgacatc
    13561 gccaaccaca aaaacaagtt cgatcgcggc ttccgcaacc tcatcgatgt cttcggcgcc
    13621 gaccacggtg gctatatcaa gcgcatgcag gccgccgtta aagcagtcac gtcaggcgag
    13681 gccacgctgg atgtgaaggt cgtgcagctc gtcaggctgt tgcgccacgg cgaaccggtc
    13741 aagatgtcga aacgctcggg cgagttcgtc acgctgcgcg aagtcgtcga cgaggtcggc
    13801 tccgacgcgg tgcggttcat gatgctgttc cgcaagaacg atgcggtgct ggattttgac
    13861 cttgccaagg tgatcgagca gtccaaggac aacgcggtgt tctatgtgca gtacgggcat
    13921 gcccgcggcc actcggtgtt ccgtaacgcc cgggaggttg ttccggacct gccggaggag
    13981 gccagcgccc gggcggcgtt tttgggggac gcggcgatgg agcggctgac cgaccctggg
    14041 gaaatcgacc tgctgcggcg gctggcggcc tatccccgga tgatcgaggc ggcggccgtg
    14101 gcccatgaac cgcaccgaat cgccttttat ctgtatgatt tggccagcga atttcacgcc
    14161 ttatggacca gggggcgcga tctgccctat ttacgcttca ttatcaataa tgatgcagac
    14221 atcacaaggg cgcggctggc tttggtccag ggcgtcgtct cggtgctagc gtcagggctc
    14281 gccgttctcg gcgtcgacgc tccggccgaa atgagataga acacggcgaa cgccgctcac
    14341 gtggggacct gtgagggcac ctggcaaggg ccagttcgtt tttaaaagtg ggttggcggc
    14401 ttacccgaag ggcacgcatc atcacgatgg ctgatcgata tcaggataaa ccgtttccag
    14461 cgggcgacga tcatgatcgt cgcggtgacc agcttgcgcc tggaagggct gaaggcgatc
    14521 cgctggccga actcgcgcgg ctgatcggtc agaccgatcc gttcgccggc atgggccggg
    14581 ccaatcagca ggtaccgccg cgtggcggtg agcgcgatca ataccaacgc ccggtggccg
    14641 cagatgaggc ccccgccgca aggccgccgc agtggatgca gcgcgccacc cggcaggaag
    14701 tcccgcagcg gcaggaagtc cctcaacggc aagaagtccc gcaacggcaa gaagtcccgc
    14761 agcggcagga actcccgccg gtggagtacc cgccggaaca cccgagcgcc gtccatccac
    14821 tgcatcgcca cgcgcccccg cgtgcaacac tggagccacg ttatcacgag gcgccgccgt
    14881 tcgccgacga cgatcaggag ccggagcccg atccgtcgcg ctatgacgaa gcgctgtacg
    14941 gtcagctcga ccacggcgcg gaggagcctc agtacgatca agcctatgcg gaggaagatg
    15001 acccgtacgg ctaccaggac ggctatggcg acggcgatgg caccgaggag cgagtgccca
    15061 agcgcagccg tggcatcatg accattgcca tcgtgcttgc gctggcggtg gtgggaacag
    15121 gcgcagcgtt tgcctatcgt acctttgtcg gagcgtcccg cagcggcgag ccgccgatca
    15181 tcagggccga cgccggcccg accaagatcg tgccgtcgcc tgccgacgcc acgaccaagg
    15241 tgccggaccg catggtggca ggtgatggca ccgagaggat cgtcccgcgc gaagaagcgc
    15301 cggtcgacgt caatgccaaa tccggcccgc gcgtggtgtt tccgccgctg aacccgaacc
    15361 cgaatccgcc ggccgcatcg agtgtagctt caaacgctct gccgccggca agcggcggca
    15421 atggcacgct ggcgagcaat gagccgcgaa agatcagaac cctttcggtc cgcggcgacc
    15481 aacctgatgc cgccgcgacg ccagttgggg cggcgccacc ggcaaagccg acaccggcca
    15541 cccgctcggt ggccaacgcc aatgcttcga ccaatgcgcc gttggcgttg acgccgcagc
    15601 agtcggctgt tccggttgtg gaaccccgaa cgcgggtcgc tgctagcacc cccacggatc
    15661 aggccgcgcc cactggcggc ggatacttgg tccaggtgtc ctcgcagcgc aatgaagccg
    15721 atgcgcaggc ttcgttcaag gcgttgcagg gcaagttccc ggcggtactg ggatcgaagg
    15781 atccggtgat caagcgcgct gatctcggcg aaaagggggt ctattaccgc gccatggtcg
    15841 gtccattcgg gtcgtcagat gaagcgtcgc agttctgcgg cagcctgaag accgccggcg
    15901 ggcagtgcgt cgtccaaaag aattgacggc cgtatgctcg gttgttgctg acgcgtcagc
    15961 cttgttgacg cttcttcagg ctccttgacc cccgggccac ccgcgggcta atcggccggc
    16021 atgagttcgc gcgcattcat cacaggcgtt tccggaccgg agctaaccgc cgccgagcgc
    16081 gaattcatcg ccgcggagcg gccctggggc tttattctat ttaagcggaa tatcgagacg
    16141 ccggcgcagg tcattctgct ggttcgggaa ttacgcgcag cgatcggcaa tccagatgca
    16201 ccggtactga tcgaccagga aggaggccgg gtccagcgcc tcggcccgcc gcactggccg
    16261 gtttacccgc cgggagcggc ctttggggtg ctttacgaca tcgatcgcgc ggccggcctg
    16321 accgcggcac gcctgagcgc gcggctgatg gcggcggacc ttattgagct cggcatcact
    16381 gtcgattgcc tgccgctggc ggatgtgccg gttgccggcg ccgacgcggt gattggtaac
    16441 cgggcctacg gaaccgagcc ggataaggtc gcggccatcg ctcgcgccgt gacggaggga
    16501 ctggaacagg gcggcatatt gccggttctc aagcatattc ccggacatgg acgcgccact
    16561 gcggataccc atttccggct tccggaggtc gatacctcga aagcggagtt ggaacggacc
    16621 gattttgccg cgtttcaacc gcttgcggac ctgccgatgg cgatgaccgc acatgttgtg
    16681 tttagcgcgt tagatcccgc ccaaccggcg acgacttctg cgacaatcat ccaacaggtg
    16741 attcgcggcg ggatcgggtt ccagggtttg ttgatgagtg atgatgtgtc catgaacgcg
    16801 ctggcgggat cgatcgccga gcgaacccgg gccatcattg ccgccggctg cgacatggtt
    16861 ctgcattgca acggcaagct cgacgaaatg cgcgaggtcg cgcgcgaaac gcccgaactg
    16921 tccggcaagg cgctggagcg tgccgggcgg gcattggcgt cgcgtcgggc accgcaagcc
    16981 ttcgatcggc ttgcggggcg ggctgaactg gacgcgttga tcgatcgggc aggggccaaa
    17041 ggcgcatgag cgcggaaatc ttgtcattcg aaaccggacg ccccgccgag ctcgccgaaa
    17101 acgagcccgc gctggtcgtc gacgtcgagg gctatgaggg cccgctcgac ctgctgctgg
    17161 cgctggcgcg gcaacagaag gtcgatctcg ccaagatttc cattctggcg ctggcggacc
    17221 agtatctggt gttcatcgag gcggcccgga aaatccgcct cgagcttgcc gccgactatc
    17281 tggtgatggc ggcatggctc gcctatctga aatcgcggct gctgctgcct gaaccggcga
    17341 cggcggacgg accgagcgcc gaggagatgg cgaccgcgct ggccaaccgg ttgcgccggc
    17401 tcgaggccat tcgcgaggca tcgaaccggc tgatgaaccg cccgcaattg cagcgcgaca
    17461 tttttccgcg cggcgatccg gaatcgatcg ccgagatcaa acatcccaaa ttcaccgcaa
    17521 ccctgttcga cctgctcacc gcctatgccg cgcagcgcca gcagcgcgtg ctggctagcg
    17581 tgcatctgac gaaacgcacg gtgtggtcgc tgagcgaagc gcgggcctca ctggagcgtc
    17641 tcgtgggctt ggcggaggat tggagccggc tcgaccagta tttgctcgcc tacatcgtcg
    17701 atccctcgca aaaggccacc gtgttcgcgt caagctttgc cgctgcgctg gaactggtcc
    17761 gggagggcgc ggtcgagttg caccagaaac aggcgttcgc gccgctgtat tttcgaaagc
    17821 gtccgccgca accgggatcc gacgcgatca ccgcgccgga tctgccagag taagtggaag
    17881 cgagtaagtg gaagaaggag accgtgccat ggcaagcctg gcggaaaaac gcaacgccga
    17941 tgccgaggag gattcagtgg atccgcaagc gcggcccgag gaattgcggc tgttggaagc
    18001 gttgctgttc gcctcgagcg agccgctcga tcaggccgcg ctcgccaagc ggatgcccga
    18061 cggcgtcgac gtcaaggtgg cgctggcaca attgcaggcg gattacgcat cgcgcggcgt
    18121 caatctggtg cgcgttgcca ataaatggac cttccgcacc gcgggcgatc tgtcctggct
    18181 gatgacgcgg gagaccaccg agacgcgccg gctgtcgcgc gcggcgatcg aaatgctcgc
    18241 gatcatcgcc taccatcagc cgatcacgcg cgcggagatc gaggatatcc gcggcgtcat
    18301 cacctccaaa gggacgctgg atgtcctgct ggaaaccggg tggatcaggc cgcgcggccg
    18361 ccgcaagacg cctggacgtc cgctgacatt cggaaccacg gaagcctttc tgtcgcagtt
    18421 cagtctggag gcgttgggcg acctgccggg ccttgaggaa ctgaaaggga ccgggctgtt
    18481 ggattcgcgg ttgcccaccg gcttcagcgt tccgtcgccg tcggacgacc cggctttgcg
    18541 cgatgacgag gacccgctgg aaatcggcga tctggaactc gcgctggcgc cggcagtgga
    18601 gccggaatcc gaggacggaa gctgatttag ctccaacccg cgatagcctt ggcgaccaat
    18661 taacggtagc ggcaatgcgc cgcgtcaaca gcgatttgac gcggcctaag tccttgtttt
    18721 tcgcaagccc caagcatact ttccgggaag attaggctgg gtttccggcc catgtgtttg
    18781 gagggttgca ggatgggttc gcttagcatt tggcactgga tcgtggtgat cgcagtcgtc
    18841 ctgctgttgt tcggccgcgg caagatttcc gacctgatgg gcgacgtcgc gcagggcatc
    18901 aaggccttca a
//
LOCUS       DASWGM010000692         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1875835,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000692 DASWGM010000000
VERSION     DASWGM010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875835"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VGA15_19460"
     CDS             <1..449
                     /locus_tag="VGA15_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589910.1"
                     /translation="VTDNPLFVAIAPSTMADIKPGMFVGSAGTMQPDGTQKAIEVHIF
                     PESMRGTGEGHYDWDLKPQTKMTNGNVEQTVAGVDGPLLSVKYKDGEKKLVVTPETVV
                     VTYVPGNKDDLKPGTRIFVAAAKKQPDGTLQTPRITYGRNGAGPAF"
     gene            519..1073
                     /locus_tag="VGA15_19465"
     CDS             519..1073
                     /locus_tag="VGA15_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX9589911.1"
                     /translation="MRSIVSLTLAACAIAVMSSSTLAQAPAPATPPVPPAPPSAGGTP
                     DAMPFDIPYGLSIGLEHAKQVMAAAEAEAKKRNWKMNIAVVDTNGELVQFERMEGAQI
                     ASGSISVGKARTAARFRRESRLFYNAFETGHGYVSTLDPTLVASPGGFPLVEGGKLIG
                     AVGCSGGTGDQDAVVCKAGAEVVK"
     gene            complement(1194..>2031)
                     /locus_tag="VGA15_19470"
     CDS             complement(1194..>2031)
                     /locus_tag="VGA15_19470"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9589912.1"
                     /translation="NDIIARIFGQKLSESLGQPVIVENKPGGGAIVATEYVAKSAPDG
                     YTLLVGASGMAINPAVYARLPYDSIRDFVAVSELASFPLILIVNPSSPIKSVAELVAY
                     AKANPDKANYASSSASFQLVTELFKQKTGAPMQVIPYKGANDSVMAVISGQVTAAIAD
                     AGPALSQVLGGQARALAVAAPKRMEELPDVPTLKEAGADVDAVLWSGLFAPKETPPVI
                     VKMLESEIMRIARLPEVSSRLKPLGILSVGNSSEEFGRILAADIKRWAEVAKAGNIKI
                     EQ"
BASE COUNT          404 a          613 c          633 g          381 t
ORIGIN      
        1 ccgtcaccga caatccgctg tttgtcgcaa tcgctccctc gaccatggcc gacatcaagc
       61 cggggatgtt cgtcggctcg gctggaacga tgcagccaga cggcacccag aaggcgatcg
      121 aggtgcatat cttcccggaa tcgatgcgcg gcaccggcga aggccattac gactgggatc
      181 tcaagccgca gaccaagatg accaatggca atgttgagca gacggttgcc ggggtcgatg
      241 gaccgctgct gtccgtcaag tacaaggatg gcgagaagaa actggtggtg acccctgaga
      301 ctgttgtcgt gacctacgtc cccggcaaca aggacgacct caagccgggc accaggattt
      361 tcgttgcggc cgcgaaaaag caacctgacg gcacgttgca aaccccgcgt attacctacg
      421 gcagaaacgg cgcgggaccg gcattttgac ttaagattta gcgtcgcggc actatcgaca
      481 gcccttacaa cggaagcaga cttcaactgg aggatactat gcgttcaatc gtttcgctga
      541 cactggcagc ctgcgccatt gcggtcatga gttcgtcgac acttgcgcaa gcgccagcgc
      601 cagcaacgcc gccggtgcca ccagcgccgc cgtccgcggg cggtacgccc gatgcgatgc
      661 cgttcgatat tccctatgga ctgtcgatcg ggcttgagca cgccaagcag gtgatggcgg
      721 cggctgaagc ggaggccaaa aagcgaaact ggaagatgaa catcgccgtg gtcgatacca
      781 acggcgaact cgtgcagttt gagcggatgg agggcgcgca gatcgcatcc ggttcgattt
      841 cggtcggcaa ggcgcgcacc gcggcacgct ttcgccgcga atcccgcctg ttctacaacg
      901 cgtttgaaac cggccatggc tatgtcagca cactcgatcc gaccttggtc gccagtcccg
      961 gtggatttcc gcttgtcgaa ggcggcaaac tgatcggcgc ggttggctgc agcggtggca
     1021 ccggcgatca ggacgcggtg gtatgcaagg ccggcgctga ggtcgtgaaa tagcacggcg
     1081 tttttctgcc ccggcgctgc acagcgccgg gtttcgctgg cgccgcggcg agacattgca
     1141 cgcggcgcca acatgactgt tattgtcgcg cttgcccgac cggcagggga gggttactgc
     1201 tcgatcttga tattgccggc cttggcgact tcggcccagc gtttgatatc ggcagccaga
     1261 atccggccaa actcttcgga cgaatttccg accgagagga tgccgagcgg tttcaagcga
     1321 gagcttacct ccggcaatcg cgcgatgcgc atgatctcgc tctcaagcat tttgacgatc
     1381 acgggcggcg tctccttggg cgcgaagagg ccgctccaca gcaccgcatc gacatcggcg
     1441 ccggcctctt tcaaggtcgg cacatcaggc agttcctcca tccgcttggg cgccgcaacc
     1501 gcgagcgcac gcgcctggcc tcccagcacc tggctcaacg ccggaccggc atcggcgata
     1561 gcagcggtga cctgacctga gatcaccgcc atcaccgaat cgttggcgcc cttgtaggga
     1621 atgacctgca tcggcgcgcc cgtcttttgc ttgaacaact cggtcaccag ctgaaacgag
     1681 gcggacgagc tggcataatt tgccttgtcc ggattggctt tggcgtaggc gacaagttcg
     1741 gcgaccgatt tgatcggcga agatgggttg acgatcagga tcagcggaaa cgatgccaat
     1801 tcggagactg caacaaagtc gcggatcgaa tcgtacggca gcctggcgta gacggcgggg
     1861 ttgatggcca tgccgctggc gccgaccaat agcgtgtagc cgtccggcgc cgacttcgcg
     1921 acatattcgg tggcgacgat cgcgccgccg cccggcttgt tttcgacaat gacgggctgg
     1981 ccgaggcttt cggacaattt ttgcccaaaa atccgcgcga tgatgtcgtt g
//
LOCUS       DASWGM010000693         8260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_475532,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000693 DASWGM010000000
VERSION     DASWGM010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475532"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..934
                     /locus_tag="VGA15_19475"
     CDS             83..934
                     /locus_tag="VGA15_19475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9589913.1"
                     /translation="MASIGAWASGISESLIAIPRITRLRPLISGAAELSDERKSPGEL
                     SGTVELSRVTFRYTTNGPPVLENVSLRIAQGEYVAIVGPSGSGKSSVFRLLLGFEKPE
                     SGTVFLDGKAIDTLDIGAVRRQLGVVLQNGKLTTGSLYDNICGGVQLPLQQAWEAARL
                     AGLDDDIKAMPMGMNTVVAEGVNTLSGGQRQRIMIARAVARRPRILLFDEATSALDNR
                     SQAIVSTSLGNLNVTRIVIAHRLSTVRQADRIIVLVDGKVVQTGSFAELSNTPGTFAS
                     FAQRQLL"
     gene            1224..2192
                     /locus_tag="VGA15_19480"
     CDS             1224..2192
                     /locus_tag="VGA15_19480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9589914.1"
                     /translation="MPPNRTSYAVDIVVPCYLEQDVLPITVPAVQNYLRGLAQQGNYK
                     LASCRLILVDDGSSDSTWDAIRKFNSLHPEIAGIKLSRNCGHQSALLAGLAHASADVI
                     ISMDADLQDDIHAIGKMLLAYEAGSDLVLGVRSDRTSDTVTKRGTAGAYYKILSLLGV
                     NIVENHADFRLMSKRALAALLLHDEVNLFLRGIIPTLGFKTSIVPYSRQPRAAGETKY
                     TLRKMAHLALDGVTSFSIAPLRFVSGVGLTIFLVSMSIGLYYFGERIFAPESVIPGWA
                     STVLPVLFLGGLQLLSIGVLGEYIGKIYLEVKRRPRFIVEETTFPS"
     gene            2189..3754
                     /locus_tag="VGA15_19485"
     CDS             2189..3754
                     /locus_tag="VGA15_19485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9589915.1"
                     /translation="MKRTVATAIIGLLVMISAALNLSFALRSGSLSRPPVYDDVVYLL
                     DAYQRLAFGGVNSLRTLVNSFLTQPPHAPMSTLTAMLGYSLIGPYLWAAYAANVWMLG
                     LFAAAVYLVARQNMDRIPSILVVALMLFVPAAHALITEFRPDQGAGVILACALYSLVS
                     TDYAKATRPRLVALGFLVVCAVMAKPSAIVATVPVLGLAAAIGILRPGYYSSSEAIYS
                     IRAAILAVAVAIAILIPCAVLWGPVTYAYIYQALVTNKDIWGADGGQLYHWMFNSFGA
                     GGHQALGPFLWLGLSSIMVDALLSFSAKPHRSNYDTLAFYFVTTILYSGIAVSSQKTV
                     YQGSLFYFPFLLATTLAVGRILMRIGGRAPIAITGVLLATAIIFMPATSFYQFYPEFQ
                     DSAPILVSISNDVADEVLRKRTSGCAADRFVLAAMNPDPIPPEAVALDLAMRRRVKIA
                     PLTPYTVRSAAEMDHAVDGADFVILSRAGEHSNLPGSKFGAHTSLRLSTSADWRRIAA
                     SGDYELFAKAKCR"
     gene            complement(3930..4727)
                     /locus_tag="VGA15_19490"
     CDS             complement(3930..4727)
                     /locus_tag="VGA15_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEX9589916.1"
                     /translation="MKRILGAVMVTAALTAPSLAADLGARSYTKAPALAPVSNWSGFY
                     VGGNLGYGWGNGNTDFSFLPDPTAFNFNNTTLGARSTGVIGGAQAGYNWQIGSLVTGL
                     EADIQGSGIKGSVSAAPTILGTSLPTTDGTLISSEPKLSWFGTVRGRLGVTVTPDLLL
                     YGTGGLAYGHVDASANYFEPNDPGFAPLVAPASVSKTKVGWTAGAGAEWMFAHNWSAK
                     LEYLYLDLGSDSATGSFAPPVPPQVQVGYTWHTRENITRVGVNYHFN"
     gene            complement(4951..5196)
                     /locus_tag="VGA15_19495"
     CDS             complement(4951..5196)
                     /locus_tag="VGA15_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9589917.1"
                     /translation="MENNRAAPRQRTLKAGTIEFSGSTIDCVVRNISETGAALEVASP
                     VGIPAEFNLLISGNVAKRPCRVVWVKDKRIGVAFKSG"
     gene            complement(5773..6507)
                     /locus_tag="VGA15_19500"
     CDS             complement(5773..6507)
                     /locus_tag="VGA15_19500"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEX9589918.1"
                     /translation="MSRQSEPDTGAAAEAVPDCPHCQKPMPLCICDSITPIENKVALL
                     ILQHPQEQDRALGTARLTALHFANAVLKIGLSWPSLSKALGRPVHDPSRWAVLYLGSA
                     KVAELDTDRDIVAINRKGEIEDNQRAILKDIEGIVLLDGTWSQAKALWWRNAWMLKCQ
                     RVILGPRHPSRYGQLRREPRRDGLSTIEAAAMLLANLEKRPDIAETLHGSFEQMLAKY
                     REAQSVLPELAPKPKPKRDYRRRKRA"
     gene            complement(6580..>8260)
                     /locus_tag="VGA15_19505"
     CDS             complement(6580..>8260)
                     /locus_tag="VGA15_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9589919.1"
                     /translation="VVSLTITPMICAHYIKQATSHNATWFDRVVEGSLSRIVAFYART
                     LRVVLGFPFLTLIVFFATIALTVVLYIKTPKGYFPTDDSGFVIGATRASADISFQSML
                     GLQQQLADIVMADPAVAGIGSVLGGGGGPGGAATSNRGTMFISLKPPAERAGLSTEQA
                     IDRLRKNLFRVAGIRLFMFAAQDLRAGGRQSDSDYQYTLTSTDLDLLQKWAPIVGKRM
                     ESVEGITDVSSDRDPGGLQLTLSIDRKAASSLGVRVQDIDNALNNAFSQRQISIVYTQ
                     RNQYMVVLEIDPKFQSDPSNLEHIFVAGAGGAQVPLSAVVHYQRGLSALAVFHSQSFP
                     STTVSFNLLPNVPLDVATSNIQRAVEELHMPEGIRGSFDGNAGDFGKTSGHMPLLILG
                     ALVAMYIVLGVLYESLAHPLTIISTLPSAGLGALLALQLTNTPLTVIAFIGIILLIGI
                     VKKNGIMMVDFALDAERHRGLSSADAIFEACSARFRPILMTTMAALFAGIPLVIATGP
                     GTELRRPLGITIIGGLFVSQILTLYTTPVIYLLIDRLRRRPGPAAIAAPAE"
BASE COUNT         1563 a         2541 c         2412 g         1744 t
ORIGIN      
        1 cgcggcggct actttcctca agagcaaact gctgcaggat gtcggcggct ttctcgcctt
       61 tttcgccgca ttcggacaat cgatggcatc gatcggcgcg tgggcttcgg gtatcagtga
      121 atcgctgatc gccatcccgc gtatcacccg tctccggccc ttgatttccg gcgcggccga
      181 gctgtctgac gaacggaaat ccccgggcga gctgtccgga acggttgagc tatcccgggt
      241 gacctttcgc tacacgacga acggcccgcc agtgctcgaa aacgtctctc tgaggatcgc
      301 gcaaggcgaa tacgtcgcca tcgtgggccc gtcaggcagc ggaaaatcct cggtgttccg
      361 cctgctgctg ggcttcgaga agcccgagtc cggcacggta ttcctcgacg gaaaggctat
      421 cgacacgctc gatatcggcg cggtccgccg tcagctcggc gtcgtattgc agaacgggaa
      481 actgaccacg ggtagcctct atgacaatat atgcggcggc gtccaactgc cgcttcagca
      541 ggcttgggag gcggcgcggc tcgccggcct cgatgacgac atcaaggcga tgccgatggg
      601 catgaatacc gtcgtcgctg aaggcgtgaa taccctatcg ggtggccagc ggcagcggat
      661 catgatcgct cgcgccgtcg ctcgccgccc gcgcattctc ctgtttgacg aggcgacgag
      721 cgcgctcgat aaccggtcgc aggccatcgt gagcacctcg ctcggcaacc tcaatgtgac
      781 ccggatcgtc attgcacacc ggctcagcac cgtgcgccag gcggatcgca ttatcgtgct
      841 cgtcgacggc aaggtcgtgc agaccggaag ctttgcggag ctcagcaaca cgcccgggac
      901 atttgcttcc ttcgctcagc gacaattgct ttagtgatat aaatcacgcg cgaatggcct
      961 ttttcgcgat gtgacgccct tgcgcttcgc tagtccttcg ccgtcatcag gctggacaga
     1021 ggacttccac ctccaagctg tcgttcatac tcggcacacc aaaaaagaag cccggcagct
     1081 tgccgggctt acccgttcgg tggagatcgt ggtggcaaac gaatctgccc cgtgaacggg
     1141 cgccctcacc tctcctcctt ccggatgacg ccgatgtcca ttgtttcggg tagtttccac
     1201 cgcatcaacc agttggaata cctgtgcccc cgaaccggac cagttacgcc gttgatattg
     1261 ttgttccctg ctaccttgaa caggatgttc ttccaatcac ggtgccagcg gtccagaact
     1321 acttgcgcgg cttggcgcag caaggaaatt acaagctggc gtcctgtcgg ctaatcttgg
     1381 tcgatgatgg gagtagtgat tcaacctggg acgccatacg gaaattcaat tcactccacc
     1441 ctgaaatcgc aggcatcaaa ctctcccgca attgcggaca ccaatcagcc ctgctggctg
     1501 gcctggcaca cgcatcagcc gatgtcatca tttcgatgga cgctgatctg caagacgaca
     1561 tccacgcaat aggaaagatg ctgctggcgt atgaggcagg gagtgacctt gtactcgggg
     1621 tccgctccga tcgcacaagc gacactgtca cgaagcgcgg cacagcggga gcctactata
     1681 aaatcctctc gcttctgggc gtcaacatcg tcgagaacca cgccgacttc cgcctcatgt
     1741 caaaacgagc gttggcggcc cttctgcttc acgacgaggt aaacctcttt ctaagaggca
     1801 tcattccaac tctgggcttc aagacttcaa tcgtccccta ctcgcgacag ccgcgcgccg
     1861 ctggcgagac aaaatacaca ctgcgcaaga tggctcatct tgctctcgac ggggtcacat
     1921 ctttctccat agcgccgctt cgtttcgtct caggcgtcgg cctcaccatc tttctagttt
     1981 cgatgtcgat cgggctttac tatttcggcg agcgtatttt tgcaccggaa agcgttatcc
     2041 cagggtgggc atctaccgtg cttccggtcc tgtttcttgg cggtcttcag cttttgtcga
     2101 tcggcgttct cggtgagtac atcggcaaga tataccttga agtgaagcgt cgacccagat
     2161 tcatcgtaga agaaacgaca ttcccatcat gaagcgcacc gtagcgacgg caatcattgg
     2221 attgctggtt atgatttcgg ctgctctaaa tttgtcattt gccttgcggt cagggtcttt
     2281 atcgaggcca ccggtttacg acgatgtcgt gtatctttta gacgcctatc agagactggc
     2341 attcggcggc gtcaattcgt tacgcaccct ggtcaattcc ttcctgaccc agcctccaca
     2401 cgctcccatg agcactctga cagcgatgtt ggggtactcg ctgatcgggc cttacttgtg
     2461 ggccgcctat gcggccaacg tctggatgct cggcctgttc gctgccgccg tatatctcgt
     2521 tgcgcgacag aacatggatc ggattccaag cattctggtc gtcgcattga tgctttttgt
     2581 tccggcggcc cacgccctga tcaccgaatt tcgtccagat caaggagcgg gtgtcatcct
     2641 ggcctgcgct ctgtattcgc tcgtttctac agattacgcg aaggcgacaa gaccgcgact
     2701 tgtcgcgctg ggatttctcg ttgtgtgcgc cgttatggcg aaaccatctg caatcgtcgc
     2761 gaccgtcccg gtcctgggcc tagccgccgc gatcggcatt ctccgccctg gctactattc
     2821 cagctcagaa gcgatttact ccattcgggc ggcaattctt gccgttgccg tcgccatcgc
     2881 gatcctcatt ccctgtgctg tcctatgggg accggtcacc tacgcctaca tctatcaagc
     2941 actcgtcacc aacaaggata tatggggcgc cgatggcggc cagctttatc actggatgtt
     3001 caattccttt ggcgcgggcg gccatcaagc ccttggaccg ttcctatggc tcgggctatc
     3061 cagcatcatg gtcgacgcgc tgctttcatt ttcggcaaaa ccgcatcgga gtaactacga
     3121 tacgcttgcg ttctatttcg taacgacaat tctctattcc gggattgctg tttcgtcaca
     3181 aaaaacggtc tatcaaggca gcctttttta ttttccgttt ctgttggcaa ccactctggc
     3241 ggtggggcgc atcctgatgc ggattggcgg ccgtgctccg atcgcgatca ctggtgtatt
     3301 gctggcaacg gccatcattt tcatgccggc cacgtcgttt tatcagttct atccggaatt
     3361 ccaggattca gccccgatac tcgtatcgat ctcgaatgac gtagcagacg aagtgcttcg
     3421 taagcgcacc agcgggtgtg cggccgatcg tttcgtactc gcggcgatga acccggaccc
     3481 aattcctccc gaggctgttg ccctggattt ggcgatgcgc cggcgcgtca aaattgctcc
     3541 ccttacgcct tacacggtga ggtctgcagc cgaaatggac cacgctgttg acggcgcgga
     3601 tttcgtgatc ttgtcaaggg ccggggagca ttccaatctg cccggctcaa agttcggcgc
     3661 ccatacgtcg ttgcgacttt cgaccagcgc cgattggcgc cgaatcgcgg catccggtga
     3721 ctacgaattg ttcgccaaag ccaaatgcag atgaccgttg ggcgctcccg tatgcgccgc
     3781 ttatggcgaa tgcggagcta tttacacccg tataagtacc atcagcgtca gtcaggcaca
     3841 cgcaccgatt ggccacgaca gatttttcga aaaaagcccg gcaaggtgcc gggctttctc
     3901 gaatctggtc caggcggccg cggccgccat cagttgaagt ggtagttcac gccgacgcgg
     3961 gtgatgtttt cccgggtatg ccaagtgtat ccgacctgga cttgtggggg taccggagga
     4021 gcgaaacttc cggtcgcaga gtcactgccg aggtcaaggt aaaggtactc aagcttggcc
     4081 gaccaattgt gcgcgaacat ccattcggcg ccggcgcccg cggtccagcc gaccttggtc
     4141 ttgctgacac tggcaggcgc gacgagggga gcgaagccag ggtcgttagg ttcgaagtaa
     4201 tttgccgaag cgtcaacatg gccatatgcc agaccgcccg tcccatagag caacaggtcg
     4261 ggcgtgacgg tgacgccgag acggccccgc accgtgccga accatgaaag ttttggttcg
     4321 gacgaaatca gagttccgtc ggtggtcggc agactggtac ccaaaatggt tggtgccgcg
     4381 ctaactgagc ctttgatgcc cgagccctgg atatccgcct ccaggccggt caccagcgag
     4441 ccgatctgcc agttgtaacc tgcttgcgcg ccgccgatga cgccggtcga tcgagccccc
     4501 aaagtcgtgt tgttgaagtt gaaagccgta ggatctggca ggaacgagaa gtccgtattt
     4561 ccattgcccc agccatagcc gagattgcca ccgacataga agccgctcca gttgctgacc
     4621 ggcgccagcg caggcgcctt ggtatagctc cgcgcgccaa gatcggccgc caagctcggc
     4681 gctgtcagcg ccgccgtcac catcaccgca cccaaaatcc gcttcattat gaccccctac
     4741 aatcgtgccc tgagatggcg ccgcactgac ggcttgctct cgaatctcag cgcaagagtt
     4801 acggggaagc gggtcctgcg acttggacag gcgcgacggt ggttttgact aaaacgaccg
     4861 tccggcgatt ggcggccact gatgcatttt tgccgatgcc cacgcgccct cgcccggccc
     4921 ttaggtgcgc ttgcggcaga tcggtcctag ctagcctgac ttgaacgcca cgcctatgcg
     4981 cttgtcttta acccagacca cccggcatgg acgcttggcg acattgccgg agatcaagag
     5041 attgaattcg gcgggaatac ccaccgggct cgcgacctca agtgctgcgc cggtctccga
     5101 aatgttccgg acgacgcaat caatggtgct gccgctgaat tcgatagtgc cggctttcaa
     5161 tgtacgctgt cgtggcgctg cccggttatt ctccagactt cggagttcct cgggttttgg
     5221 ctgcccgtct acgttggacg gttgctgctc ggccagtttc tcggcgtgcc gcgatgcgat
     5281 cgcgatggcc tcttcggtga gcccggccct gatcctcgtc cacgcgatct tgaaccgaag
     5341 ctggcagtcc ttgaaattga tcccgggtcc ccggtcatca gccgccgacg gctggccgta
     5401 gacattgcag ccccaccacc aatgcgggtc gccgggcggc ttcatgatcc tgccgatcgg
     5461 caggccgcgc caaatcgcct cgtagtcgtc ttcctgccgc ttcccgtcaa tgacggtcgg
     5521 acgcaacgcg acggattccg aatcgtcggt catcacctga cgtgccttca accggtctcc
     5581 cgcgcccgtt ccgccgaagc aggccgcggt tttacctcgc aatcgaattt gcgcagattg
     5641 cctgaatttg tctgcgatag actacgtaca agttatcaaa gcagggtttt ttctggcggg
     5701 aaaccggctt ccacctggcc ggaaaattct cagggatagg ccgggttcca tccggcgaac
     5761 ccatcggctg gatcaggcgc gcttgcggcg gcggtaatcg cgcttgggtt ttggttttgg
     5821 cgcaagctcc ggcagcacgc tctgcgcctc ccgatatttt gccagcatct gctcaaagct
     5881 gccgtgcagc gtctcggcga tgtcgggccg cttttcgaga ttggcgagca gcatcgcggc
     5941 cgcctcgatg gtggacaggc cgtcgcggcg cggctcgcgg cgcaactgcc cgtagcgcga
     6001 cggatgcctg gggccgagga tgacgcgctg gcatttcagc atccaggcat tgcgccacca
     6061 cagcgccttg gcctggctcc aggtgccgtc aagcagcacg atgccctcga tgtctttcag
     6121 gatggcgcgc tggttgtcct cgatttcgcc cttgcggttg atggcgacga tatcccggtc
     6181 ggtatcgagc tcggcgactt ttgccgaacc gaggtaaagc accgcccagc gcgagggatc
     6241 gtggaccggc cgtcccagcg ccttggacag gctcggccac gacaggccaa tcttgagcac
     6301 cgcattggca aaatgcaatg cggtcagccg cgccgtgccg agcgccctgt cctgctcctg
     6361 cggatgctgc aggatcaaca gcgcaacctt gttctcgatc ggcgtgatgc tgtcgcaaat
     6421 gcacagcggc atgggttttt ggcagtgcgg acagtccggc acagcctccg ctgcggcgcc
     6481 ggtgtcgggc tcggattgtc ttgacatggg cccgctatac gctctgagga cgtgcgcttc
     6541 aaccactcag ggtcgatcgc tcaagctatt ctgaaggcgc tattccgcgg gcgccgcgat
     6601 cgcggcgggc ccgggccgtc gccgcagccg gtcgatcaag agatagatca ctggcgtggt
     6661 gtagagcgtc aggatctgcg atacgaacaa tccgccgatg atggtgatgc cgagcggccg
     6721 gcgcagctcg gtgccggggc cggtggcgat caccagcgga atcccggcga aaagtgctgc
     6781 catcgtcgtc atcagaatcg gccggaaccg ggcgctgcac gcctcgaaaa tggcatcggc
     6841 cgacgacagg ccacggtgac gttcggcgtc gagggcaaaa tccaccatca tgatgccgtt
     6901 cttcttgacg attccgatca atagaatgat gccgatgaag gcgatgaccg tcagcggcgt
     6961 gttggtcagc tgcagcgcca agagcgcgcc gaggccggcc gacggcagtg tcgagatgat
     7021 ggtgagggga tgggcgaggc tttcatagag caccccgagc acgatataca tcgctaccag
     7081 cgcgccgagg atcaagagcg gcatatgccc gctggtcttg ccgaaatcgc cggcattgcc
     7141 gtcaaaactg ccgcgaatgc cctcgggcat gtgcaattcc tcgaccgcgc gctggatgtt
     7201 cgatgtggcg acgtccagcg gcacattggg cagcagattg aacgacaccg tggtcgacgg
     7261 aaacgactgc gaatgaaaca ccgccagcgc cgacaacccg cgctggtaat gcaccacggc
     7321 ggacagcggc acctgggcgc cacccgcgcc ggcgacgaag atatgttcga ggttggacgg
     7381 atcgctctgg aatttcggat cgatctccag caccaccatg tactggttgc gctgggtata
     7441 gacgatcgag atctgccgct gcgagaacgc gttgttgagc gcgttgtcga tgtcctgcac
     7501 gcgcacgccg aggctcgatg ccgcttttcg gtcgatcgac agcgtcaatt gcaggccgcc
     7561 gggatcgcgg tcgctggaca cgtcggtaat gccttcaacg ctctccatgc gtttgccgac
     7621 gatcggcgcc catttctgca acagatcgag atcggtgctc gtcagcgtgt attgatagtc
     7681 ggaatcgctc tgtcggccgc cggcgcggag atcctgcgcc gcgaacatga acagccggat
     7741 ccccgctacc ctaaagagat tcttgcgcag ccgatcgatc gcctgttcgg tgctcaatcc
     7801 ggcccgctcg gccggcggct tgagggagat gaacatggtg ccgcgattgg aggtcgccgc
     7861 cccgccgggg ccgccgcctc cgccaagcac cgatccgatc cccgccacgg ccggatcagc
     7921 catcacaatg tccgcgagct gctgctgcag ccccagcatc gactgaaacg agatatccgc
     7981 ggacgcccgc gtggccccga tcacaaaacc cgaatcgtcg gtcgggaaat atcccttggg
     8041 ggtcttgata tagagcacca cggtcaaggc gatggttgcg aagaagacga tcagcgtcaa
     8101 aaacggaaaa cccagcacca cccgcagcgt ccgcgcatag aatgccacga tgcgcgacaa
     8161 cgagccttcg acgacacggt cgaaccaggt cgcattgtgg gaagtggctt gcttgatgta
     8221 gtgggcgcag atcatcggcg tgatcgtcag cgacaccacg
//
LOCUS       DASWGM010000694         6735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1027559,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000694 DASWGM010000000
VERSION     DASWGM010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027559"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VGA15_19510"
     CDS             complement(<1..843)
                     /locus_tag="VGA15_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457000.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9589920.1"
                     /translation="MTSVTDAPENPLPFGRSRSRRTGSGGAIGWLRANLFSSIPSTAI
                     SLLLIFLLAKACMSLVQWGYWNAIWTVPGNQTNACRAIRGLGACWAVIPEKYRFILFG
                     TYPFDQQWRPALAILTFIALFFLSSRQSWWRKELVLVWVAALVVIGLLMWGGIFGLPY
                     VSQDRWGGLPVTLILATFGLAFGFPLGIVVALGRRSKLPAIRSLCVLYVELIRGVPLI
                     SLLFMASVMFPLFMPDGVNIDKLLRAQIAFVLYAGAYLAEVMRGGLQAVPRGQHEAAD
                     ALGLS"
     gene            complement(847..2034)
                     /locus_tag="VGA15_19515"
     CDS             complement(847..2034)
                     /locus_tag="VGA15_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315607.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9589921.1"
                     /translation="MTTSTPEPARRKRLPISLSHERLAGLFWQIVVVGIAIAVVAWLW
                     SNAVHNLSARRISTGFAFLGREAGMPIADAWLAYSPKNTYLRAFIVGIVNTLRVAVIG
                     IVVATVLGTMIGIARLSSNWLLSRLAAVYVEVLRDLPLLLQLLFWYVLMQGLPAARAA
                     WMPMDGVYLSNRGLVLPSIPIEEGNLWVIGTAAVGLVALYALRRRLIARQLLDGQPRP
                     LWPYVLVLAVGPPALVSWGLGVSWTITMPELRGFNFVGGLTLTPEYFALLVALVTYTS
                     AFIAEIVRSGIQAVPRGQWDAAMALGLRRSFVLQHIVMPQALRVIIPPMTSQYLNLIK
                     NSSLAVAVGYQDIVSIANTTLNQTGQAIEAIALIMLVFLTISLGISLLMNWYNARVAL
                     VER"
     gene            2133..3008
                     /locus_tag="VGA15_19520"
     CDS             2133..3008
                     /locus_tag="VGA15_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX9589922.1"
                     /translation="MTQPHLKSSPLNELCSALPSLPMRLREVGQFVAANDYDATTRSM
                     RDLAAAAGADPASFTRLAKALGYSGWDGLRAALTEARRPAQPSPFSGRTKGRRQGSNS
                     DVSLVADKLEAEAAGLARISPGAVAGAAKALHAAQRIWIAGFRSCRGVAELLNYQLRL
                     FRPDTVQLVGGSGPEDLDLGAFRAGDAVVVIGFTPYSTASVLSARAAHRSGARLIAIS
                     DTVAAPMAEGADHLLLFEAAASPGFFPSLTGAIAIAQSLAAVTFVLGGVGSKRRLEET
                     EARLAAMSQYVAEKG"
     gene            3014..4363
                     /locus_tag="VGA15_19525"
     CDS             3014..4363
                     /locus_tag="VGA15_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9589923.1"
                     /translation="MGVSPSRVLHRTLRETPPKAVGGDGVWLIAEDGRRILDGSGGAA
                     VSCLGHQHPRVLEAIARQASKLAYAHTGFFSSEPAEALADQLVGDEPGGLAYAYFVSG
                     GSEAIEASIKLVRQYFIEIGQPQRQRFIARRQSYHGNTLGALSAGGNAWRREPYAALL
                     SPAFSHVTPAFAYHEKRDDESEADFVARLAAELEAEFQRLGPDTVAAFMAEPVVGATA
                     GCVPPPEGYFRAVRDICNRHGALLILDEVMCGMGRTGTLHAWQQEGIAPDIQAIAKGL
                     GGGYQPIGAMLASADIIDTIRQGSGAFQHGHTYLAHPLACAAALEVQRIIGEERLLER
                     VRDLGAHLERRLTERFGNHRHVGDIRGRGLFQAIELVADRASREPFDPGLKLNQRIKA
                     AAFESGLACYPAGGTVDGRRGDHVLLAPPYVATSDDIDMIVDRLGSAVDSALKTVGK"
     gene            4377..5390
                     /locus_tag="VGA15_19530"
     CDS             4377..5390
                     /locus_tag="VGA15_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9589924.1"
                     /translation="MKRIVIATGLLATWFSGATAGTLDTVKQRGTLVCGVSAGFAGFS
                     TPDSQGNYKGLDVDYCRALAAGVLGDANKVRYVALTAQNRFTALQSGEIDVLYRNSTQ
                     TYLRGVTLGLRQGPVNFYDGQGFVVRRDSGVKDLKGLNGATVCVAQGTTHEVTLGDYG
                     RANGIEWKPLVFDRIDTMYQTFFGGRCDAMTQDASALAGAVATAAPNAADYLVLPLTI
                     SKEPLGPFTRNSDELWTDIIAWLHYGLIEAEELGVTASNADEMAQSTNVPAIQRLLGN
                     SGELGSRLGLDNKWMVQAIKAGGNYAEIFERNVGQTSPLKLNRGLNATWSKGGLMYAI
                     PFK"
     gene            5449..6087
                     /locus_tag="VGA15_19535"
     CDS             5449..6087
                     /locus_tag="VGA15_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX9589925.1"
                     /translation="MRITLVHALKHSIAPIEASFAGLWPDARLMNLLDDSLSADLARD
                     GGLTDAMTGRFLSLGRYAASTGANAILFTCSAFGPCIDAVAREHAPMPVLKPNEAMIE
                     QAVAQGRRIGLLSTFAPTLVSMPPEFPRSVEIVPKLVTGALAALDRGARAEHDRLVAE
                     ASRELRDCDLIALAQYSMAPAAALVADASGRPVLTTPDSAVLKLKELLAVVG"
     gene            complement(6198..>6735)
                     /locus_tag="VGA15_19540"
     CDS             complement(6198..>6735)
                     /locus_tag="VGA15_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9589926.1"
                     /translation="VWDGAVRIDGRDVTRFPPHRKRALGLAYAPQEQALFQDLSVRDN
                     LRLGLADDRSFEARLADVSGWFPVLTARLSQRAGTLSGGEQKMLIVARGLIAEPRLFL
                     MDEVTEGLQPSVVDRLADVLATTRRERGTAMLVVEQHLPFVLGLADRFAVFKRGEVVD
                     AGPVDAGSAVRIDEHMRL"
BASE COUNT         1331 a         2162 c         2100 g         1142 t
ORIGIN      
        1 cgagaggccg agcgcgtcgg ccgcctcgtg ctgtcccctg ggaacagcct gcaagccgcc
       61 gcgcatcact tccgcaagat aggcgccagc gtaaagcacg aacgcgatct gggcccggag
      121 cagcttgtcg atgttgacgc catcaggcat gaacagcgga aacatgacgc tcgccatgaa
      181 cagcaggctg atcagcggaa caccgcggat gagttcgaca tagagcacgc aaagtgaccg
      241 gatcgctggc aacttcgaac ggcggccgag cgcgacgaca atcccgagcg gaaagccgaa
      301 ggctagcccg aatgttgcga ggatcagcgt caccggtagc ccgccccagc gatcctggga
      361 aacatagggc agcccgaaga tgccgcccca catcagcaag ccaatcacga ccagcgcagc
      421 gacccacacc agcacgagct ccttgcgcca ccagctctgc cggcttgaca ggaagaagag
      481 cgcaatgaac gtgaggatcg caagagcggg ccgccattgt tgatcgaagg gatacgttcc
      541 gaacagaatg aagcgatact tctcggggat aaccgcccag caggcgccaa gtccacggat
      601 cgcccgacaa gcattggtct gattgccggg aaccgtccag atcgcgttcc agtaacccca
      661 ctgcacgagg ctcatacacg cctttgcgag caggaagatc agcagcagcg agatggcggt
      721 cgagggaatc gacgagaata gattggcgcg cagccatccg atcgcgccgc cgcttccggt
      781 cctgcgcgat cgcgatcgac cgaagggaag cggattttcc ggcgcatccg tcactgaggt
      841 catcgatcag cgctccacca gcgcgacgcg cgcattgtac cagttcataa gtagactaat
      901 gccaaggctg atggtcagga acaccagcat gatgagcgca atggcttcga tggcctggcc
      961 ggtttggttc agcgtcgtat tcgcaatcga cacgatgtcc tgatagccga ccgcgacagc
     1021 cagcgatgaa ttcttgatca aattcagata ttggctggtc atcggcggaa tgatgacgcg
     1081 aagtgcctgc ggcatgacga tgtgctgaag aacgaagctc cggcgtagac cgagcgccat
     1141 ggcggcatcc cattgccccc tgggaacagc ctgtatgccg ctgcgtacga tctcggcgat
     1201 gaaggccgag gtataggtga cgagcgccac caggagcgcg aagtattccg gcgtcaacgt
     1261 caatccgccg acaaaattaa agcctcgcag ctccggcatg gtgatggtcc aggatacgcc
     1321 caggccccac gacaccagcg ctggtggtcc caccgctaga acaagcacgt aaggccagag
     1381 gggacgcggc tgaccatcca gcagctgccg cgcgatcaat cgccggcgca gcgcatataa
     1441 cgcaaccagc ccgaccgcgg cggtaccgat cacccaaagg tttccttcct cgatcggaat
     1501 cgacggaagc accagacctc gatttgaaag atatacgccg tccatcggca tccatgcggc
     1561 gcgggccgct ggaagtccct gcatcagcac gtaccagaac aaaagctgca gcagcaacgg
     1621 aagatcacgg agcacttcga catagaccgc tgccagccgg gacagcagcc aattggacga
     1681 cagccgcgcg atgccgatca tcgtcccgag cacggtcgca accacgatgc cgatgacggc
     1741 gacacgaagc gtgttgacga tgccaacgat gaatgcgcgc agataggtgt tcttggggct
     1801 gtaggcgagc caagcgtccg caatcggcat cccggcctcg cgaccgagaa aggcaaagcc
     1861 ggtcgagatg cgccgcgccg acagattgtg aactgcattc gaccagagcc aggcgacgac
     1921 agcaatcgcg atccccacca ccacgatttg ccagaaaagt cctgcgaggc gctcatggct
     1981 cagtgaaatg ggcagccgct tgcggcgagc aggttcgggc gtggatgtcg tcacagcaca
     2041 aggatccttt tgtcagcagc gatcttcggc catgcgcaaa cgagcgcgct tcatatgttg
     2101 cacataaagt cattttatgc aacatacgtt tcatgaccca accgcacttg aaatcctcgc
     2161 ccctcaatga actctgcagc gccttgccgt cgctgccgat gcggttgcgg gaagttgggc
     2221 agtttgtcgc cgccaacgac tatgacgcca cgacgcgttc gatgcgcgac ctggccgccg
     2281 cggcgggcgc cgatcccgcc tccttcaccc ggctggcgaa ggcgctgggt tattcgggat
     2341 gggacggact ccgcgccgcc ttgaccgagg cgcgccggcc cgcgcaaccg tctccgttct
     2401 caggccgaac gaagggacgg cgccagggtt ccaattccga cgtctcgctg gtcgccgaca
     2461 agctcgaagc cgaggcggca gggcttgcgc ggatctcgcc tggcgccgtg gccggggcag
     2521 ccaaggccct gcatgcggca caacgtatct ggattgccgg ctttcgaagc tgccgcggcg
     2581 tggcggaatt gctcaactac cagcttcggc tgttcaggcc cgataccgtg cagctggttg
     2641 gcggctcggg tcccgaagat ctcgacctcg gcgccttccg tgccggcgac gccgttgtcg
     2701 tcatcggctt tacgccctac tcgacggcaa gcgtattgtc ggcccgtgcg gcgcatcgct
     2761 ccggcgccag actgattgcg atcagcgaca cggtcgccgc accgatggcg gaaggcgccg
     2821 atcacctgtt gctgtttgaa gccgccgcct cgcctggatt tttcccgagc ctgaccggtg
     2881 cgattgcgat cgcgcagtcg ctggccgcgg tcacgttcgt gctgggtggc gtgggctcga
     2941 agcggcgcct cgaagagacc gaagcccgcc tcgcggcaat gtcacaatat gttgctgaga
     3001 aaggttgagc ttcatgggcg ttagccccag ccgcgtgctg caccgaaccc tgcgcgaaac
     3061 gcctccaaaa gccgtcggcg gcgacggcgt gtggctcatt gccgaggatg gccgccggat
     3121 tctcgatggc tccggcggcg ctgccgtttc ctgtctcgga catcaacacc cgcgcgtgct
     3181 cgaggccatc gcaaggcagg cgtcgaagct cgcttatgcc catacaggct tcttctcgtc
     3241 ggagccggca gaggcgctgg ccgaccaact cgtcggcgat gaacctggcg gcctcgccta
     3301 tgcctacttc gtcagcggcg ggtcggaagc gatcgaagcc agcatcaaac tggtgcggca
     3361 atattttatc gaaatcggtc agccacaacg ccagcgcttc atcgcccgtc gccagagcta
     3421 ccatggcaat acgctcggcg cgttgtcggc cggcggaaac gcctggcgcc gcgaacctta
     3481 tgcggcattg ctgtcgccgg ccttcagcca cgtgacgcct gccttcgcct atcacgagaa
     3541 acgcgacgac gaatcggaag cggatttcgt ggcgcggctg gcggccgagc tggaggctga
     3601 attccagcgc ctcggcccgg acacggtggc cgcattcatg gcagagccgg tggtcggcgc
     3661 caccgccgga tgtgtgccac cgcccgaagg ttacttcagg gccgtgcgcg acatctgcaa
     3721 ccggcacggc gccctgctca tcctcgacga ggtaatgtgc ggcatgggcc gcacgggcac
     3781 gcttcacgcg tggcaacagg agggcattgc gcccgacatc caggccatcg ccaaaggact
     3841 cggcggcggc taccagccga ttggcgcgat gctcgcaagc gctgatatca tcgacacgat
     3901 ccggcagggc tcgggagctt tccagcacgg acatacctat ctcgcgcacc ctctcgcctg
     3961 cgccgcggcg cttgaggtgc agcgaattat tggtgaggag agactgctcg aaagggtcag
     4021 ggatctcggc gctcacctgg agcgacggct gaccgagcgc ttcggtaatc accggcacgt
     4081 cggcgatatc cgcgggcgcg gcctgttcca ggccatcgaa ctggtcgccg atcgcgccag
     4141 ccgcgagccg ttcgatcctg gcctcaagct caaccagcgg atcaaggccg ccgcgttcga
     4201 gagtggcctc gcctgttacc cggcaggcgg gacggtggac ggccgccgcg gcgaccacgt
     4261 cctgctcgca ccgccctacg tcgcgacatc agacgacatc gacatgatcg tcgacaggct
     4321 cggctctgcg gtagacagcg cattgaaaac tgttggcaaa taggaggatg tgcacaatga
     4381 agagaatcgt cattgctaca ggattgctcg cgacatggtt ttcgggagca accgctggaa
     4441 cgctcgacac ggtcaagcag cgcggcacgc tcgtctgcgg cgtcagcgcg ggctttgccg
     4501 gattctccac tccagactcc cagggcaatt acaaggggct cgacgtcgat tactgccgcg
     4561 cgttggcggc gggggttctc ggcgacgcca acaaggtgcg ctatgtcgcg ctcacggcgc
     4621 aaaaccgatt caccgcgctg caatcgggcg agatcgacgt gctctatcgc aattcgaccc
     4681 agacctatct gcgcggcgta accctcggcc tgcggcaggg gccggtcaat ttctatgacg
     4741 gccagggttt tgttgttcgg cgcgacagcg gcgtgaagga tctgaaggga ctgaatggcg
     4801 ccaccgtttg cgtcgcgcag ggaacgaccc acgaggtgac gctcggcgat tacggtcgcg
     4861 ccaatgggat cgaatggaag ccgctggtgt tcgatcgcat tgacaccatg tatcagacgt
     4921 ttttcggcgg gcgttgtgac gccatgaccc aggatgcctc cgcgctcgca ggcgcggttg
     4981 caacggcggc gccgaacgcc gccgactacc tcgtgctgcc gctgacgatc agcaaggaac
     5041 cgctcgggcc gttcacgcgc aatagcgacg agctgtggac cgacatcatc gcctggcttc
     5101 actatggcct catcgaggca gaggaactcg gcgtaaccgc atccaatgcg gacgaaatgg
     5161 cacaatccac caacgtacca gccatccagc gactgctcgg caactccgga gaactcggat
     5221 cgcgccttgg cctcgacaac aagtggatgg tccaggcaat caaggcgggc ggtaactacg
     5281 ctgagatctt cgagcgcaac gtgggccaga cgagcccgct gaagctcaat cgcggcctga
     5341 acgcgacctg gagcaaaggc ggcttgatgt acgccatccc cttcaaatga gggcccgcag
     5401 ctcggcgggt catattgaca taagctgcgc ccggccagga ttaacaagat gcgcatcact
     5461 ctcgtccatg ccctgaagca ttcgattgcc ccgattgagg cgtcgttcgc cgggctttgg
     5521 ccggacgccc gcctgatgaa tttgctcgac gacagcctgt cggccgactt ggcgcgtgat
     5581 ggcgggctta ccgatgcaat gaccggacgc ttcttgtcgc tcggacgtta cgcggccagt
     5641 accggtgcca acgcgatcct gttcacatgc tcggccttcg gcccttgcat cgacgccgtc
     5701 gcgcgcgagc acgcgccgat gccggtgctc aagcccaatg aagcgatgat cgagcaggcc
     5761 gtcgcgcagg gccgcaggat cggcttgctc tcgacgttcg caccgacact ggtttcgatg
     5821 ccgccggaat ttccccgctc cgtcgagatc gtgccaaaac tggtgaccgg cgctctcgca
     5881 gcgctggatc ggggggcgcg cgccgaacac gaccgtctcg tggcggaagc gtcacgggag
     5941 ttgcgcgact gcgatctgat tgcgctagcg cagtacagca tggcacctgc ggcggcgctg
     6001 gtcgccgacg cgtcgggtcg gccggtcctc acgacaccgg atagcgccgt gctgaagttg
     6061 aaggaattgc ttgccgttgt cggttgacaa gagcgggatg acttttcttc gaatcgtcat
     6121 cccgctctat ctttttgttt gagcatgatc tgttcggaaa accggcaccc acttttccgg
     6181 atcatgctct aggggcgtca caggcgcatg tgttcgtcga tccgtacggc agagccggca
     6241 tcgaccggcc cggcatccac cacttcgccg cgcttgaaca ccgcaaatcg atccgcaagg
     6301 ccgagcacga acggcagatg ctgctcgacc accagcatcg cggtgccgcg ttcgcgacgc
     6361 gtcgtggcca gcacgtcggc gagccggtcg acgaccgatg gctgcaggcc ttcagtcacc
     6421 tcatccatga gaaacagacg cggctcggcg atcagtccgc gcgccacgat cagcatcttc
     6481 tgttcgccgc ccgacagcgt gccagctcgc tgggacagtc gcgcggtcag gaccggaaac
     6541 catccgctga catcggccag ccgagcctcg aagctgcgat catcggcaag ccccagccga
     6601 aggttgtcgc gcacgctgag atcctggaac agcgcctgtt cctgcggggc ataggcaagc
     6661 ccaagcgcgc gcttgcgatg cggaggaaac ctggtgacat cgcggccgtc gatgcgcacg
     6721 gcgccgtccc acacc
//
LOCUS       DASWGM010000695         9701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_614458,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000695 DASWGM010000000
VERSION     DASWGM010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614458"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VGA15_19545"
     CDS             complement(<1..1071)
                     /locus_tag="VGA15_19545"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HEX9589927.1"
                     /translation="MQDWPIFASIVSHSLRAAQQEIRHGGSMPSIVIAQGGGPTAVIN
                     QTLCGAILAARRHDPSLRILGARYGVRGLTAGNVVDLTTIPEAQLRRLGNTPNSALGS
                     TRDKPDSGACADILTALDRLDARAFVYIGGNDTAGTLELLRQQSSGPCHFVHAPKTID
                     NDLMENDHVPGFISAAAFVANALVRVDLDFRAMPGIYVAIVMGRHAGFLAAAAAGWQQ
                     SPDDAPKLIYTPEKPFSVVQFLDEVDAIYARLGRCIVSMSEGVQDETGRPLAEALACE
                     AVERDAHGNLQLTSGDLGIEIQKALKSRFPKARARVDTLGYLPRGYIGVIDETDRKEA
                     FAAGAFAAQSALTGSGSVVLHYD"
     gene            complement(1331..3133)
                     /locus_tag="VGA15_19550"
     CDS             complement(1331..3133)
                     /locus_tag="VGA15_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589928.1"
                     /translation="MKRRVIVVDGPLAFGMRRFQAARANDVGLEVLTLPLLASRLAGG
                     FRHRADRETLSMAVARALRDGGFAQLDAVRNLPGMVRAALQSLDRAWSADLDLDNLAS
                     TSARVSDLAMLQQRVSEVLPQGAMLPRQLRDAALERVSFAPKLFGSVELDKLVDVEPL
                     WRPLLTALSAQLEVSWAATVELDRGWFPGRVSLAEPAAPTAMRGELCADPRAEVVEAL
                     RWTRELLSRGDASAGDIAITGAATPAWDEHILALARQAGIPVHFSHGLPALNSWEGQS
                     CAALADVLGNGVNQERVRRLLMHSSSSAMGALPDDWAAGLPQQAGLFTVDQWRRALQS
                     ARGRRSDPDAAERTLIPMLELLATGIANAEQAGKSFLGGASLGLWKDALRNAPPAAIA
                     MSLESLRVRDDRHPANSVVWCPASHLVAAPRRWMRLLGVAGRTWPRSESEDPLLPDQV
                     LSRRRLIPVSITQRDRRCFDLLVTQPAEEIVVSRSRRSADGALQSASALWPATAPVRT
                     CARTRIPEHAFSEADRLLARPAEAGRSGRIKAALTCWRNWDREEATPHDGAVRAGHPA
                     VARAVSRLHSATSLMLMARHPLGFLWRYALDMNS"
     gene            complement(3145..4170)
                     /locus_tag="VGA15_19555"
     CDS             complement(3145..4170)
                     /locus_tag="VGA15_19555"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9589929.1"
                     /translation="MMPVLDTCATLRFSTEGMPEERRAQAVRELHLHERTILSARLEP
                     IEPLDPLPDYPLHVDLTKRTLPGLAVVSGTLSGLRQAIRPSRAVANGEDDLLLGVNVS
                     GCSTAHQRDRDLVLRDGDAFFATRDESGFTVTRPTPVRFVGCRVPREAVAPLVGRIDD
                     TPLRLVPPRSEALILLVTYARAIADALPFATPELQRLAVTHMHDLIAATIGATRDGRA
                     IAEGRGIAAARLRSIMADITANLGHGDLSVADVARRHRVTPRYIHKLFENEGLTFSSF
                     VLGQRLSRAHRTLSDPRFADRNISSVAFDVGFGDLSYFNRTFRRRYAATPTDIKQSSM
                     TTDPPRR"
     gene            4341..5468
                     /locus_tag="VGA15_19560"
     CDS             4341..5468
                     /locus_tag="VGA15_19560"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9589930.1"
                     /translation="MAKIETILVVGGGVAGLTTAAALHWHGFTMELVERQQSWHSLGA
                     GFLVQANGMRMLLSLGLAAGVEDAGAVVRRWQFCDEQGQVLSETDLEALWGDAGPCVG
                     IERSKLQRALLPGVGHLRCRLGTSVISVVQDHRRVSVGFSDGSTEDYDVVVGADGIRS
                     TVRALTLTAAAPNDLGAMNWRSIAPIRPAGLTGLQMHMGDGCMFGLVPMGAGSTYGFA
                     YVILPRFHDPLEGRLDRLRNRFATFGGRVKEYLASLERDDQVICSAMEWMEFEKWHTG
                     RVVLVGDAAHASSPMMGQGGCMAMEDACVLAEELRAAATVESALASYVSRRKARVEWV
                     QHQSMAVGESLIIPSAVRNAMLRQRGNEAMHARFGPLVSAP"
     gene            complement(6490..6867)
                     /locus_tag="VGA15_19565"
     CDS             complement(6490..6867)
                     /locus_tag="VGA15_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589931.1"
                     /translation="MLRLGNARALLVAALIAGAMGPAYPDDFGGVRILAYGNNTCGSF
                     VKSDPQVKQMYLAWVVGFITGSNAYAAGRDRLVGEHWDPNSTLLWLENYCSKDPLATF
                     FTAADDFRRALAIKEGVQRKDTP"
     gene            complement(6899..7336)
                     /locus_tag="VGA15_19570"
     CDS             complement(6899..7336)
                     /locus_tag="VGA15_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9589932.1"
                     /translation="MFPTPAVSTTDGRTELQTVLEFLRPGDVLMVSRLDRLARSIGDL
                     QDIVRISATMKATEQPFNTGDIYGELTVNLLGCFAQFETALRKERQLEGIAKAKAEGV
                     YKGRKPSVDVAQVRALKAQGVGPASIAKTLGIGGASVYRALVT"
     gene            7638..7817
                     /locus_tag="VGA15_19575"
     CDS             7638..7817
                     /locus_tag="VGA15_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589933.1"
                     /translation="MAEYRAYILDEDGHVSSAIEFVCADDEAAKKYARQFVDGHDVEL
                     WQRDRQIATFDCEPE"
     gene            8166..8423
                     /locus_tag="VGA15_19580"
     CDS             8166..8423
                     /locus_tag="VGA15_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589934.1"
                     /translation="MNTIIILRRPERPADLIRRPIRDGLIPGLVLTLFLVLVQFEASK
                     ECRGGAFSAGFGGGGFDVHRCDLVVRRIGSDRGLRIPLPQH"
     gene            complement(8761..9516)
                     /locus_tag="VGA15_19585"
     CDS             complement(8761..9516)
                     /locus_tag="VGA15_19585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX9589935.1"
                     /translation="MTINGDEGTPITAYVAKPSGPGLFPGVVLVHHLPGWSEFYIETT
                     RRFAHHGYLAICANLYERAGQGNPDDVAAKVRADGGISDAQMVGDTEAAVKWVRAQSN
                     HNGKVGVFGSCSGGRHAFIYACQRNDVDACVDLWGGRVVMGKEELNAKTPVAPVEMTK
                     DLSCPLLGIFGNDDSAPSPEQVNQHEGELKRHGKMHEFYRYDGAGHGIFYWHRPLYRP
                     EQAMDGWSKVFTFFGNTGGWEAGGERVFTEDRV"
BASE COUNT         1804 a         2972 c         3118 g         1807 t
ORIGIN      
        1 gtcataatgc agcaccaccg agccgctgcc ggttaaggcg ctttgcgcgg caaatgcgcc
       61 agcggcgaaa gcctccttgc ggtcggtttc gtcgatcacg ccgatatatc cgcgtggcag
      121 atagcccagc gtatcgacac gcgcccttgc cttgggaaaa cgcgatttca gcgctttctg
      181 gatctcgatg ccgagatcgc cgctcgtcaa ttgcagattg ccatgggcgt cgcgctcgac
      241 cgcctcgcac gccagagcct cggcgagagg ccggccggtc tcgtcctgca ctccctcgga
      301 catcgagacg atgcagcggc ccaggcgcgc ataaatggca tcgacctcgt cgaggaactg
      361 gacaaccgag aacggctttt ccggcgtgta gatcagtttc ggggcatcgt cgggcgattg
      421 ctgccagcct gcggcggcgg cggcgaggaa gccggcatgg cggcccatga cgatggccac
      481 atagatgccg ggcatggcgc gaaaatcgag atctacgcgg acaagggcat tggcgacgaa
      541 ggcggcggcc gagatgaagc cggggacatg gtcgttctcc atcaggtcgt tatcgatggt
      601 cttgggggca tgcacgaaat ggcaggggcc acttgactgt tgacgcagca gttcgagcgt
      661 tccggcggtg tcattgccgc caatatagac gaaggcacgg gcgtcgagcc ggtccagagc
      721 ggtgaggata tcagcgcatg cgccagaatc tggtttgtcg cgggtcgagc cgagagccga
      781 attgggcgtg ttgccgaggc ggcggagttg ggcctcgggg atggtggtta ggtcgacgac
      841 attgcccgcc gtgaggcctc gtacgccata gcgcgcgccc aggatgcgca gggagggatc
      901 gtgcctgcgc gcggcaagga tggcgccgca cagggtctgg ttgatcacgg cggtggggcc
      961 gccgccctgg gcgatgacga tcgatggcat ggatcctcca tgacgtattt cttgctgtgc
     1021 cgcccgcaga ctatgagaga cgattgaagc gaagattggc caatcctgca acgggctgtg
     1081 tggcctgcgg cgaaaggatc ttccaggcag agtgcaacgg cgtcgtcttc atcatgaacg
     1141 ctgcaagcgt cgccgaggcg cacgagatcc tggaagcgct ctccttaatc tcggttcggc
     1201 tatccaagtt tcgatggcat tccgtgtctg ctcggtgatc ccgaaccgaa ctggtcgtcc
     1261 cgtctttttc tgcactgctg tcgctcgatc gcggacccgt ccgccggagc tgatatcgtc
     1321 aaggcgagtt tcacgagttc atgtctaagg catagcgcca caggaagccg agcggatggc
     1381 gtgccatcaa cattaatgat gtcgccgaat gcagtcgcga cactgcgcgg gccaccgccg
     1441 gatgccctgc gcgcaccgcg ccgtcgtgcg gggtcgcctc ttcccgatcc caattgcgcc
     1501 agcaggtcag cgccgccttg atgcggccgg accgtcctgc ctccgcaggt ctagccagca
     1561 gccggtcggc ctcgctgaac gcatgttcgg gaatgcgcgt gcgcgcgcat gttcggaccg
     1621 gcgcggtcgc cggccacagc gcgctggcgg attgcaacgc tccgtcggcc gagcgtcggc
     1681 tgcgcgatac gacgatctcc tccgctggct gtgtaaccag cagatcgaaa catcttcggt
     1741 cgcgctgcgt gatcgagaca ggaatcagcc ggcggcgcga aaggacttga tcgggcagga
     1801 gagggtcctc gctctcggat cgaggccaag tcctaccggc aacgccgaga aggcgcatcc
     1861 agcggcgcgg cgcggcaacc aaatgggacg caggacacca gacgacgctg ttggctggat
     1921 gacggtcatc ccggacacgc aggctctcga gcgacattgc gattgcggcc ggcggcgcgt
     1981 ttcgaagggc atccttccac agaccgaggc tcgcgccccc gagaaagctt ttgccggcct
     2041 gttccgcatt cgcaattccg gtagccagca attcgagcat cggaatgagc gttcgttcgg
     2101 cggcatccgg gtcggagcgt cggcctctcg cggattgcag cgcccgccgc cattgatcga
     2161 cggtgaatag tcctgcctgc tgcggaaggc cggccgccca gtcgtcgggc agggcgccca
     2221 tcgcgctcga cgacgaatgc atcagcaatc gcctgacgcg ttcttggttc acgccgttgc
     2281 cgagcacgtc ggccaacgcc gcgcacgact gcccttccca tgaattcaga gcgggaagac
     2341 cgtgggaaaa atgtactgga attccggctt gtcttgccag cgccagtatg tgttcgtccc
     2401 aggccggcgt ggcggccccg gtgatcgcga tgtcgccggc gctcgcgtcg ccgcggctga
     2461 ggagctcccg cgtccaccgc agcgcttcca cgacttcggc tctgggatcg gcgcacaatt
     2521 cgccgcgcat tgccgttggt gcagccggtt cggcgaggga cacccgtccc ggaaaccagc
     2581 ctctgtccag ttcaacggtg gcggcccagg aaacctccaa ttgcgccgac agggcggtca
     2641 gcagcggtcg ccacaacggc tcgacgtcga cgagcttgtc caattctacg ctgccaaaca
     2701 acttcggggc gaagctaacg cgctccagcg ccgcatctcg caattgacgt ggcagcatcg
     2761 ccccttgggg cagaacctcg ctcacccttt gctgcagcat cgcaagatcg gatacgcgcg
     2821 ctgacgtgga tgcgagatta tcgagatcca gatccgcgct ccaagcgcga tccagcgact
     2881 gcagagcggc ccggaccatt cccgggaggt tcctgacggc gtcgagctgt gcaaatccgc
     2941 cgtcgcggag ggcgcgtgcg acggccatcg agagcgtctc cctgtcggcc cggtggcgaa
     3001 agcctccggc gagacgcgac gcaagcaggg gtaaagtcag gacttccaat ccgacatcgt
     3061 tcgctcgggc tgcctgaaag cgcctcatgc cgaacgccag cggaccatcc accacgataa
     3121 cgcgccgttt catgagcacc tcaatcaacg gcgcggcgga tcggtcgtca ttgacgattg
     3181 cttgatatcg gtcggtgtag ccgcatagcg ccggcgaaac gtgcgattga aataggaaag
     3241 atcaccgaac cccacgtcaa acgccactga gctgatgttg cgatcggcaa agcgcgggtc
     3301 gctcagcgtg cggtgtgcgc gcgagaggcg ctggccgagc acgaacgacg agaaggtgag
     3361 gccttcgttc tcgaagagct tgtgaatgta acgtggtgtc acgcggtggc gtcttgcgac
     3421 gtcggcgacg gacagatcgc cgtggccgag attcgcggtg atgtcggcca tgatagatcg
     3481 cagccgtgcc gcggcgatgc cgcgtccctc cgcaatcgcc cggccgtcgc gcgtggcgcc
     3541 gatggttgcg gcgatgagat cgtgcatgtg ggtgacggcg agccgttgca gctccggagt
     3601 ggcgaacggc agagcgtcgg caatcgcgcg cgcataggtg acgagcagga tcagcgcctc
     3661 cgagcgcggc ggaacgagac gcagcggcgt gtcgtcgatc cggccaacca gcggtgccac
     3721 ggcctcgcgc ggcacccggc agccgacgaa gcgcaccggc gtcggacgcg tgacggtgaa
     3781 tccgctctcg tcgcgcgtcg caaaaaaggc gtccccgtcc cggagcacca gatctcggtc
     3841 gcgctgatgc gctgtgctgc acccgctgac attgacgccg agcaggagat cgtcctcgcc
     3901 gttggcgacc gccctgctag gtcgtatggc ttggcgcagt cctgacagcg ttccggacac
     3961 caccgccaga ccaggcagcg tccgtttggt cagatcgacg tggagtggat agtcgggcag
     4021 cggatcgagc ggctcgatcg gttcgagcct ggcggacagg atcgtgcgtt catgaaggtg
     4081 gagctcgcgt accgcctggg cgcgccgttc ttccggcatg ccctcggtcg agaacctcaa
     4141 ggtggcgcag gtatctagca ccggcatcat cggcgatccc ttcgcttggt ggggagcgcg
     4201 gtcggcggca agcattatcg cacgatttca ctctggtccc aatcactgtt ccgcccgatc
     4261 caagaccgca gtcccgatcc ggctaggctc cttgagccgc ggcacactgt cgatgcggta
     4321 cgccgcgatt ggagggagcc atggcaaaga tcgagacgat cctggtcgtc ggcgggggag
     4381 tagccggcct caccacggcc gcagctcttc attggcacgg cttcacgatg gagctcgtcg
     4441 agcgccagca aagttggcat tctttgggag ccgggttcct ggtccaagcc aatgggatgc
     4501 gcatgctgct ctcgctcggt ctcgcggcgg gcgtcgagga tgcaggcgcg gttgttcgcc
     4561 gatggcaatt ttgtgacgag caggggcagg tgctttcgga gactgatctc gaggcgctgt
     4621 ggggcgatgc aggcccctgc gtcgggatcg aacggtccaa gttgcaacgc gcgctgctgc
     4681 cgggtgtggg gcatctacgc tgccgactcg gcacatcggt catatccgtg gtgcaggacc
     4741 atcgtcgcgt ctcggtcggg ttctccgatg gctcgactga agactatgat gtcgtggtcg
     4801 gcgcggacgg aattaggtcg acagtacgcg ccttgacctt gaccgcggct gcgccgaacg
     4861 accttggtgc tatgaactgg cgtagcattg ccccgatccg cccggccggc ctgaccgggc
     4921 tgcagatgca catgggcgat ggttgcatgt tcgggcttgt gcctatgggt gccgggagca
     4981 cctacggttt cgcctacgtc atcctgccgc gcttccacga tcctttggag ggacgcctcg
     5041 accggctgcg caaccgtttt gcgacattcg gggggcgcgt aaaggagtat ctggcctcgc
     5101 tcgagcgtga cgatcaggtc atctgctcgg ccatggaatg gatggagttc gaaaaatggc
     5161 ataccggtcg ggtagttctg gtcggggatg cggcgcacgc cagctcgccg atgatgggac
     5221 agggcggctg catggcgatg gaagatgcat gcgtgctggc cgaagaattg cgcgcagcgg
     5281 caaccgtgga gagcgcactc gccagctacg tcagtcggcg taaggcccgc gtcgaatggg
     5341 tccaacacca gagcatggca gtaggggaaa gtctcattat tccatcggca gttcgcaatg
     5401 ccatgttacg ccaacggggc aacgaagcga tgcacgcccg cttcggcccc ctggtgtcgg
     5461 ccccttagtt cggtacacga aacagcaggc aggcggaacg gcggcgacat ggacccgctt
     5521 tcatcatctg gtcgataccg acgaagcgca cgctggcaga tgcggcaggc aaagccggcg
     5581 atgctgacgg aggtcgacat ctgctataag ttcggcgggc ttcccgtgag atggaagcgc
     5641 ttccttcgga acgtagcctg acgggtgccg cgacggctta cgcagcggac gaaaggggat
     5701 cgccccgccc gtgccacacg attcattcct gccgccgcag ggcggctggt tcctgcgaca
     5761 attaagcaga cgcagcggcg acgatgatgc ggccatcctt gttgcatcgg tcacttcaat
     5821 tgcggcagac tcccgggatg gaaacggcga atcgcggggc agttcgtgga atgatcaacc
     5881 aaaccaaaac aacggtgatt tttgcctgcc cgcaatgcaa cctggctcat gaggtgacac
     5941 agacgcactc tcgcacaaag aaggcgggag gattcgattg cgccgactgc agcgcggcgg
     6001 tccacttctg gtatggctgg tttgattgtt ccgactggag ggccgtgaac atgaattcaa
     6061 tgaccttcac gcaccccaat gccctagggg accccaacca gccgcgctga agccaagaca
     6121 attatcggct gcattccaac atggcgccgg caacgttgct cccccgcacg tcggggagca
     6181 tactccggag atgagcagcg gaacgcagac cacagtaata ttcgcctgcc ccaaatgtgg
     6241 gactgcctac gaagccacgc agcagcaaca cccggatgac cattccggca gtttcaaatg
     6301 tcaggactgc cggaccgaag tccacgcatg gtccggggtc tttagtttct ttgactggaa
     6361 ggcctatgag gccgtcggtc caaccccaga atcgtggcac aatcaaacgc cagcgaagat
     6421 gaacccctgg gcaacccgcg agcgataggc ttcgctcttc gccgaggcgc gacagcaccc
     6481 gcttgactgt cacggcgtgt ccttacgctg cacgccttcc ttgatggcaa gcgcgcgacg
     6541 aaagtcgtcg gcagctgtga agaacgtcgc gagagggtct ttgctgcaat agttctccag
     6601 ccacaaaaga gtggaattcg ggtcccagtg ctctcccacg aggcgatcgc gaccggctgc
     6661 ataggcattt gaaccggtga taaatccgac gacccaggcc aagtacattt gctttacctg
     6721 ggggtcgctc ttgacgaacg aaccgcaagt attgttcccg tacgccagga tccgaacgcc
     6781 accaaaatca tctggatatg ccgggcccat cgctccagcg atcagagcgg cgacaaggag
     6841 cgctcgggcg ttgccgagtc tcaacatgga aaaataactg agctcatgtc gaattgtctc
     6901 aggttaccag cgccctgtaa acggacgccc cgccgatgcc cagcgtcttt gcgatagaag
     6961 ctgggccgac gccctgcgcc ttgagtgcac gcacctgggc cacgtctaca ctcggtttgc
     7021 gacccttgta gacgccttca gcctttgcct tggcaatgcc ctccaactga cgttccttgc
     7081 gcagggccgt ctcgaactgc gcgaagcagc ccagcaagtt cacggtaagc tcgccgtaga
     7141 tgtcgccggt gttgaacggc tgctccgtgg ctttcatggt ggcgctgatc cggacgatgt
     7201 cctgtaggtc gccgatacta cgcgccaatc gatctagcct gctgaccatg agcacatctc
     7261 cgggccgaag gaattccagc accgtctgca gctcggtgcg gccgtcagtg gtggagacag
     7321 ccggcgttgg gaacacgacc gcgtcgctgc actggagggt agaaggcgcg gccgcgcccc
     7381 aggccgagcc cgccgtccga agcgagcacg acccagagtg gcaattcgcg cgtggctcgt
     7441 cggttccgcg aggcgcgatg accggcggcg cggcgtagcg tcgagggggc gacgggcacg
     7501 ggaatcggcg atcttttaaa ccgcgcatgg gtttgtctgt tgtcgcacca cgttttaatt
     7561 ttgttccgag tcgggaaccg aactgacaga aaacgatttc tgtgctcgat aaattgctca
     7621 gggtggggag gccgcacatg gccgagtatc gagcatacat ccttgacgag gatggccatg
     7681 tcagcagcgc aatcgagttt gtatgcgccg acgatgaggc cgcgaaaaaa tacgccaggc
     7741 aattcgtcga tggtcatgac gtagaacttt ggcagcgcga ccgtcagatc gcgacctttg
     7801 actgcgagcc tgagtgaggc cgcctcagtt ttccaaattc agtgaacgtt cggccgcttt
     7861 gtgaatcaat ttgcccaacc cggcaaaacc gttcccgttt tgagcagggg tgctccatat
     7921 tgaacacact ccgccgaacg aataggcaac tacgcactgg cccggtgggt gacgccgacg
     7981 aaaagggtac acgtcgaatg tggatcctgg cggctgcccg tcggtcgacg gtgatcaaaa
     8041 accgctggcc cacccgcgcc tgttccgggc ggggattttt ggtcagattt ttatttcggc
     8101 gcgcctgcgc gtacacaccc cctcgggggt acactgggcc gccgattcgc ccctgggccc
     8161 tgaaaatgaa tacgatcata atcctgcgtc gtcctgaaag acccgctgat ctgatccggc
     8221 ggccgatccg cgacgggttg atccccggcc tggtgctcac cctcttcttg gttctggtgc
     8281 aattcgaggc gtcgaaggaa tgtcgtggtg gcgcctttag cgctggcttt ggtggtggcg
     8341 gcttcgatgt gcaccgctgc gatctcgtcg ttagaaggat cggcagtgat cgcggactac
     8401 gtattccgct tccgcagcat tagcggtggc tacgcctcgc ttcagtggct ctcgcggacc
     8461 ctatcgacga gtcggggccg tttagcggag tgcaccaata gactgcagtt gacagctctt
     8521 attcgggaga gcccgcgcgt ggacgtgtct cggccaagcc gttgctcttg ccggcattcc
     8581 gcggcgtgcg agcgcaccgg gttatcgagc tcgtccagaa aggcgagcca gatcgtaaga
     8641 agggcgtcgt ggcgccggcg aagcgcccgc accggtgtgt caggtcgagt ccccatggac
     8701 gccagaatag cccagtatgg ttgaaagcag gtaaatggcg gaattcttgg ggtgcagaat
     8761 tcaaacccta tcctcagtaa acaccctctc tcccccagcc tcccatcccc cggtattgcc
     8821 gaagaaggtg aacaccttgc tccagccatc cattgcctgc tcgggccggt agagtggccg
     8881 gtgccaatag aagatgccgt ggccagcacc atcgtagcgg tagaactcgt gcatcttgcc
     8941 gtgcctcttg agctcgccct cgtgctggtt cacctgttcc gggctcggcg cgctgtcgtc
     9001 gttgccgaat atgccgagca gcgggcagct gaggtccttc gtcatttcga ccggcgcgac
     9061 gggcgtcttg gcattgagct cctccttgcc catcaccacg cggccgcccc agaggtcgac
     9121 gcatgcgtcg acgtcgttcc tctggcaggc atagatgaag gcgtgccgac caccagaaca
     9181 ggagccgaaa acgccgacct tgccattgtg attggactgc gcgcgtaccc acttcactgc
     9241 ggcctcggtg tcgccgacca tctgggcgtc ggaaatgccg ccgtcggcgc gcactttggc
     9301 ggcgacgtcg tctgggttgc cctggcccgc acgctcatag aggttagcgc agatcgcgag
     9361 atagccgtga tgcgcgaacc gacgcgtcgt ctcaatgtag aactcgctcc agcccgggag
     9421 atggtggaca agcacaacgc ccggaaagag gcctggacca gacggcttgg ccacataagc
     9481 ggtaatcggg gttccctcgt cgccgttgat ggtcacgttc tcgcaagtca tgcctcggta
     9541 gataacatca gtcttcctca atcggcgtca gcctagtcat ccgtcggtgc atcccagcgc
     9601 cgcaccgtca tcgataagcg gcgtcgcgct ccctttgctg ccgcggtcgc agcgcggcta
     9661 gtattcgaca atcgcgtacc gtgcgcaaca agaggcagcc g
//
LOCUS       DASWGM010000696         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_614533,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000696 DASWGM010000000
VERSION     DASWGM010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614533"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /gene="exbB"
                     /locus_tag="VGA15_19590"
     CDS             <1..479
                     /gene="exbB"
                     /locus_tag="VGA15_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HEX9589936.1"
                     /translation="LSALLSAAMREARLSAGISSDSGIKERAASSFAEIVRAEARRIR
                     LGMGVLATIGATSPFVGLFGTVWGIMNSFIGISKSQTTNLAVVAPGIAEALLATAFGL
                     VAAIPAVIIYNHFSRVTKGYLELVGRSSGAAGRLLSRDLDRTHVSSLGTTHSRAAE"
     gene            483..956
                     /gene="exbD"
                     /locus_tag="VGA15_19595"
     CDS             483..956
                     /gene="exbD"
                     /locus_tag="VGA15_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HEX9589937.1"
                     /translation="MAVSIADTELDDDSDFSETHEINVTPFIDVILVLLIIFMVAAPL
                     STVDLPIDLPSSTATPQKKPDKPTYVSIKPDLAVAIGETPVKRVDLVRSLDAMADASK
                     DRFIFLRADRAVPYGELMDVLEIMRAGGYSKIKLVALEGVPDSALPPSAPEHPKP"
     gene            987..1781
                     /locus_tag="VGA15_19600"
     CDS             987..1781
                     /locus_tag="VGA15_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX9589938.1"
                     /translation="MPDLDTEQKPSRRLWILAALGALALHIGGVALAIAHLRTDDVDD
                     SLGAPAIEIGLEMMSPHLEPTDLPPGPDADASVASPALAEQKAEVKETELPKDVPTET
                     ENPDRAVTPDDQKKPKEDDAKLAAVQTSASTESVAAEATATPSSEAIQQGPRSVAPAQ
                     GTGESAQRMRATWQKELIAHLDKHKRYPAARSQKSAEIVIGFALDRMGHVLSTSIVKG
                     SGDAAFDEAALAMVRRSDPVPQPPPLVADEGLNFTLPVIFRIKGRS"
     gene            complement(1850..>2987)
                     /locus_tag="VGA15_19605"
     CDS             complement(1850..>2987)
                     /locus_tag="VGA15_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589939.1"
                     /translation="VKVGSLGDQSGLYSDIGGPGSSVAAQMAIEDSGLLAKGWKIDLI
                     SADHQNKPDVGTNIGKQWIDVEKVDVFVDLASSGVGLAIANLAKERNVVNLNSGSASS
                     DLTGKQCSPNTVHWVYDTYMLANGTGKALVKSGGDSWFFLTADYAFGQALERDTSAVV
                     TANGGKVLGGVKHPLNNADFSSFLLQAQSSKAKIIGLANAGGDTTNSIKQAAEFGIVA
                     GGQKLAGMLMFITDVHALGLNVAQGLSFTETFYWDMNDQTRAFTKRFNERYKKSPPTM
                     VQAGVYSSLIHYFKALEALGGNPHDGAAVVAKMKEMPTDDALFGSGSIRADGRKIHPA
                     YLFEVKKPSESKYPWDYYKLVATIPADEAFLPLEKSACPMVKKS"
BASE COUNT          548 a          910 c          966 g          563 t
ORIGIN      
        1 tattgtcggc attgctgtcg gcggcgatgc gcgaggcgcg gttgtcggcg ggaatttcca
       61 gcgacagcgg catcaaggag cgcgcggcat cgagctttgc cgagatcgtg cgggccgaag
      121 cgcgccggat ccggctcgga atgggagtgc ttgcgacgat cggcgcgacc tcaccgtttg
      181 tcgggctgtt cggcacggtg tggggcatca tgaacagctt tatcggcatc tcgaaatcgc
      241 agacgaccaa tctggccgtg gtagcacccg gtattgccga agcgttgctc gccaccgcgt
      301 tcggtctggt cgcggcgatt ccagccgtca tcatctacaa tcatttttcc cgcgtgacca
      361 aaggctacct cgaactggtc ggccggtcgt cgggcgccgc cgggcggttg ctgtcgcgcg
      421 atctcgatcg cacccatgtc agttctcttg gcacgacgca ttcgcgcgca gcggagtagg
      481 cgatggccgt ttcgatcgca gataccgaac tcgatgatga cagcgatttt tcggagacgc
      541 atgagatcaa cgtcacgccg ttcatcgacg tcatcctcgt cctgctgatc atcttcatgg
      601 tggcggcgcc gctgtcgacc gtcgatcttc cgatcgattt gccgtcgtcg accgcgaccc
      661 cgcagaagaa gccggacaag ccgacctatg tcagcatcaa gccggatctc gcggtggcga
      721 tcggcgaaac cccggtcaag cgggtcgatc tggttcggtc gctggatgcg atggcggacg
      781 ccagcaagga tcgctttatc ttcctgcgcg ccgaccgcgc ggtgccctat ggcgagctga
      841 tggacgtact ggaaatcatg cgcgcgggag gttattcgaa gatcaagctg gtcgcgttgg
      901 aaggcgttcc ggatagcgcc ttgccgcctt cagcgcctga acatccaaaa ccctaaaacc
      961 ccggaaggcc aaagctgccg acagcgatgc ccgatctcga caccgaacaa aaaccgtccc
     1021 ggcggctgtg gattctggcg gcgttgggcg cgctcgcgct gcatatcggc ggcgtggcgc
     1081 tggcgatcgc gcatttgcgt accgacgatg tggacgactc cctcggcgct ccggcgatcg
     1141 agatcgggct ggaaatgatg tcgccgcatc ttgaacccac cgatctgccg ccggggccgg
     1201 acgccgatgc ttcggtcgct tcgcccgcgc tcgccgagca gaaggccgag gtcaaagaga
     1261 cggagttgcc gaaggacgtc ccgaccgaaa ccgaaaaccc ggatcgggca gtgacgccgg
     1321 acgaccagaa gaagcccaag gaagacgacg ccaaactcgc cgcggtgcaa acttccgctt
     1381 cgacggaatc cgtcgctgcc gaggcaaccg cgacgccgag ctccgaggcc atccagcaag
     1441 ggccgcgttc ggtggccccc gcgcagggca cgggagaaag cgcgcagcgg atgcgcgcga
     1501 cctggcaaaa ggagctgatc gcgcatctgg acaagcacaa gcgctatccg gcggcgcgct
     1561 cgcaaaagag cgccgaaatc gtgatcggct tcgcgctcga ccggatgggg catgtgcttt
     1621 cgaccagcat cgtcaaggga tcgggcgacg cggcgttcga cgaagccgcg ctcgcaatgg
     1681 tacggcgttc cgatccggtg ccgcagcctc cgccgctggt cgccgacgaa ggactgaatt
     1741 ttaccttgcc ggtgatcttc aggatcaagg ggcggagcta actcgaaatg aaaatgcccg
     1801 gcgatggctc gccgggcagc tccgtggcag cagctatgcg gctatcaagt cagctcttct
     1861 tgaccatcgg gcaggcgctc ttctccagcg ggagaaaagc ctcatccgcc ggaatggtgg
     1921 cgacgagctt gtaataatcc catggatatt tcgattccga tggttttttc acctcgaaca
     1981 aataggccgg gtgaatcttg cggccgtcgg cgcggatcga gcccgatccg aacaaggcat
     2041 cgtcggtcgg catttccttc attttggcga cgaccgcggc gccgtcgtgc ggattgccgc
     2101 cgagcgcctc gagcgccttg aagtagtgga tcagggacga atagacgccg gcctgaacca
     2161 tggtcggcgg gctcttcttg tatctctcgt tgaagcgctt ggtgaaggcg cgggtctggt
     2221 cgttcatgtc ccagtagaac gtctcggtga agctcaggcc ctgcgcgacg ttaagcccga
     2281 gcgcgtggac gtcggtgatg aacatcagca tgcccgcgag cttctggccg ccggcgacga
     2341 tgccgaattc cgccgcctgc ttgatcgaat tggtggtgtc gccgccggca ttggcaagtc
     2401 cgatgatctt ggccttcgag ctttgcgcct gcagcaggaa cgaggagaag tcggcattgt
     2461 tcagcgggtg cttgacgccg ccgagcacct tgccgccgtt ggctgtgacc accgcgctgg
     2521 tgtcgcgctc gagcgcctgt ccgaaggcgt agtccgcggt caggaagaac cagctatcgc
     2581 cgccggactt gaccagcgcc ttgccggtgc cgttggcgag catgtaggtg tcgtagaccc
     2641 aatgcaccgt gttcggcgaa cactgcttgc cggtgagatc agacgacgcc gagccggagt
     2701 tcaggttgac gacatttctt tccttcgcaa gattggcgat ggcgaggccg acgcccgaac
     2761 tggcgaggtc gacaaacaca tcgaccttct cgacgtcgat ccattgtttg ccgatgttgg
     2821 tgccgacgtc gggcttgttc tgatggtccg cggagatcag atcgatcttc cagcccttgg
     2881 ccaatagtcc ggaatcctcg atcgccatct gggcggcgac ggacgagccc gggccgccaa
     2941 tgtcggagta gagacccgac tggtcgccga gactgccgac cttgacg
//
LOCUS       DASWGM010000697         8895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1876091,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000697 DASWGM010000000
VERSION     DASWGM010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876091"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VGA15_19610"
     CDS             complement(<1..957)
                     /locus_tag="VGA15_19610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEX9589940.1"
                     /translation="MRPQDILIPGAAGLCCKPGGFYIDPVRPVERAVITHGHSDHARS
                     GHGAVLATQETLDMMRLRYGDNFAGRTQAIAYGEELKLGDVTIKFHPAGHVLGSAQIA
                     VSCKDTCVVASGDYKDAPDPTCAPFEAVPCDVFITEATFGLPVFRHGDAADEINKLLA
                     SVALFPERAHLVGAYSLGKAQRVIALLRLAGYDAPIYLHGAMEKITRYYESRGVALGE
                     LRLVKGIKKADLAGTITLAPPSATSDVWTRRFPDPVTAFASGWMRVRARARQRGVELP
                     LVISDHADWNGLTATIDATGAGEIWVTHGQEDALVHWCTAKGL"
     gene            1128..2060
                     /locus_tag="VGA15_19615"
     CDS             1128..2060
                     /locus_tag="VGA15_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9589941.1"
                     /translation="MPARLFLSSGDLMADRRFDFARDLQLKGDLVAAADLLLQATELA
                     PGFASAWFTLGEIRERLGERAAAIAALRRAQVADPDDRHGASLRLMLLGAEPLSSMPL
                     AYVRALFDQYAPKFEAALVDDLGYRGPALLFKAVLSVCAAFRKPALFKRAIDLGCGTG
                     LAATAFAAQVDEFTGIDLSPRMIEQARATGLYARLEVAEMVEGLRNKPDAGADLILAA
                     DAVVYLSDLAPLLCEARRVLVRGGLLAFTSETHGGDCVILGEGLRYAHSAGYLRASIE
                     ASGLALSRLEDLSARNEDNAPVPGLVVVAKKTCV"
     gene            2167..3387
                     /locus_tag="VGA15_19620"
     CDS             2167..3387
                     /locus_tag="VGA15_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9589942.1"
                     /translation="MNNNQSRRDFCATAIATILASATSVRAAEKKYDPGASDTEIKLG
                     QTVPHSGPGSLYGVLGRVGEAYFQMLNEKGGINGRQIKFFTMDDAYSAPKAVEATRRL
                     VEQEEVLALFGSLGTAPQTAVHKYLNSKGVPQLLLNTGASKWNDPKNNKWTMAGLPLY
                     PTEARILAKYVLSVKPNAKIAILYQNDDFGRDFLGPFKQVLADAGGTAKVIMEQTYDL
                     TEPTVDSQLINLSKSGADVFYNISTGKAASQSIRKVVELGWKPVQLLSAGSTGRSILS
                     AAGFENAAGIVAIRYSKEVGVPRWANDPDVMAFEELRKKYLPNVDPDNTIAFAGYGQV
                     VTMAEILRRCGDDLTRANVLRQAANLTGFHSPFFLDGITYSYTPDDYTPMKTLYISVF
                     NGKDWDISDRPVTE"
     gene            3482..6163
                     /locus_tag="VGA15_19625"
     CDS             3482..6163
                     /locus_tag="VGA15_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEX9589943.1"
                     /translation="MPSLVLPAPSAEAEPPLPDRFQRWFAARGWSPREHQLALLEKAR
                     HDRSTLLIAPTGAGKTLAGFLPTLVELSVAPRTSRLPLPLWGRSTPEGRREGGEPQAP
                     GDAAYPLPQPPDQVRGRLSTTRGEGVRSRALHTLYISPLKALAVDIARNLETPIAEMN
                     LPIKVETRTGDTPVSRRQRQRRYPPDILLTTPEQLALLLSSDDAPFLFSSLKRIVLDE
                     LHALVTSKRGDLLSLGLARLWQLAPQMHSIGLLATVAAPESLARFLVPQRDGASEVAD
                     IVVAGGAAAPVVEMLDTKERLPWAGHSARHALNEVYDLIKQNRTTLVFVNTRSQAEML
                     FQDLWRMNDDGLAIALHHGSLDVAQRRKVEDAMAKGKLRGVVCTSSLDLGVDWGDVDL
                     VINIGAPKGASRLMQRIGRANHRIDEASRAVLVPANRFEVLECAVAIDAIAENAQDTP
                     PLRTGALDVLAQHVLGCACGEPFLSDQLYAEVLTAAPYSSLTRADFDDVVDFVATGGY
                     ALKTYERFARIKQDKSGKWRVANPRTRQSYRLNVGTIVEETMLKVRLVRSRAGGSGST
                     GALGRGSRMLGHIEEAFIEGLVIGDTFVFGGEVVRYEALVEDQVYVSRAHDKDAKVPS
                     YMGGKFPLSTYLAERVRKLLDDKRAWNALPDQVRDWLSLQRDISRVPGVRELLVETFP
                     RGNKHYLVCYPFEGRLAHQTLGMLLTRRMERARVRPLGFVANEYALAVWGLGDMSFMI
                     RQGRLDLDALFDPDMLGDDLEAWLAESALMKRTFRSCAIISGLIARRFTGEEKTRRQV
                     LFSTDLIYDVLRKHQPDHVLLRAARADAATGLLDLRRLGDMLLRIKGRVVHKELDRVS
                     PLAVPVMLEIGRESVYGEASDELLAEAADELVKEATG"
     gene            6167..6874
                     /gene="pdeM"
                     /locus_tag="VGA15_19630"
     CDS             6167..6874
                     /gene="pdeM"
                     /locus_tag="VGA15_19630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEX9589944.1"
                     /translation="MTLTQYIDEQPVARVTSVDVAGVTLVADLSGALFWEEQSLLVVS
                     DLHLEKGSSFAARGVLLPPYDTVATLGRLAGAIVRHDPRMVIALGDSFHDRTAHDRLS
                     ARDRDALSALQARRDWIWISGNHDPALPCDLGGVVASEVAVGPIAFRHEPTGAAGEIA
                     GHLHPKARVSTRGRSMERRCFACDGERAVMPAFGAYTGGLSIRDVAFAKIFQTPGLMA
                     HVLGDHRLHTIAASRCY"
     gene            complement(6882..7745)
                     /locus_tag="VGA15_19635"
     CDS             complement(6882..7745)
                     /locus_tag="VGA15_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9589945.1"
                     /translation="MLDLELLRSFVSVVDSGGFTRAGERVHRTQSTVSQQIKRLEDDL
                     GQPLLNRTAKDVTPTEAGERLLSYARRLLSLAEEARDVMTRPGNEGAVRLGIPEDFAA
                     YRLAKLLATFTRARPGLRLDVRADQSVYLRRDLERGELDLALLKRDAGEKGGIAVWPE
                     RVHWVTSKSHPVDATSGSVPLIGFPPGCLYRARAIHALESAGRAWHMAYASSGLAGIQ
                     AAVAAGLGLSILSEMAIQAEHRVLTAKDGFAPIDKTEVALMASPDASPATLRLADRLA
                     EFCNTVQAKAA"
     gene            7842..8756
                     /locus_tag="VGA15_19640"
     CDS             7842..8756
                     /locus_tag="VGA15_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9589946.1"
                     /translation="MSVAPSVALPRAAFNPLPIYIALFCLLWSFAFVASKIAVTDCPP
                     LILLTARFSLAGILVLGISALRGDPWSLSPRDTCGFAILGVANNALYLGLSYTGLQSV
                     SAGLAGLIVSTNPVFTAVLAALLLKEALSWRKIAGLLLGVAGVSFIVWHRISVGADSW
                     HGILFMLGSLASIVAGTLLFKLLAPKGSLWIGTGVQNLTGGIVLLPFAFTLSSVGDIV
                     PSVRLLGAFAFLVLGGSILAYFLWFHLLNVCGATAASAYHFLMPPLGMLFAWIILGEH
                     VEVRDLMGIVPVALGIYLVTRPAAASAP"
     gene            8771..>8895
                     /locus_tag="VGA15_19645"
     CDS             8771..>8895
                     /locus_tag="VGA15_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9589947.1"
                     /translation="MNPLRITLIGGPTALIEIDGFRILTDPTFDAPGAYQLPHVKL"
BASE COUNT         1531 a         2813 c         2833 g         1718 t
ORIGIN      
        1 caggcccttg gccgtgcacc agtgtaccag cgcgtcttcc tggccgtggg tgacccagat
       61 ttcaccggca ccggtggcat caatggtcgc ggtcagtccg ttccagtcgg cgtggtccga
      121 aatcaccagc ggcagctcga cgccgcgctg gcgggcccgt gcccgcaccc gcatccagcc
      181 cgatgcaaac gcggtgaccg gatcgggaaa ccgccgcgtc cagacgtctg acgtggccga
      241 gggcggcgcc agcgtgatgg tgccggcgag atcggccttc ttgatgccct tgaccaggcg
      301 caactcgccg agcgccacgc cgcggctctc gtagtagcgc gtgatctttt ccatcgcgcc
      361 atgcagatag atcggcgcgt cgtagccggc caaacgaagc agcgcgatca cacgctgcgc
      421 cttgccgagc gaataggcgc cgaccagatg cgcccgctcc ggaaacagcg ccactgaggc
      481 gagcaacttg ttgatttcgt ccgccgcatc gccgtggcga aataccggca gaccaaacgt
      541 cgcctcggtg atgaagacat cgcatggcac cgcttcaaac ggcgcacagg tggggtcggg
      601 ggcgtccttg taatcgcctg aagcgacgac gcaggtgtcc ttgcaggaga ccgcgatctg
      661 ggccgagccc agcacatggc cggcgggatg gaatttgatg gtgacgtcgc ccagctttaa
      721 ttcttcgcca taggcgatgg cctgcgtgcg gcccgcaaaa ttgtcgccgt agcgcagccg
      781 catcatgtcg agcgtctcct gggtggccag caccgcgccg tggcccgatc gagcatggtc
      841 ggaatggccg tgggtgatca cggcgcgctc gacgggccgc accgggtcga tgtagaaacc
      901 gccgggcttg cagcacagtc cggcggcacc gggtatcagg atgtcttgcg ggcgcatctt
      961 ggctatatag tctgcctcgc acagattttt agccgtcatg cgcgggcttc acccgcgcat
     1021 ccatcttcct gcagaaccat cgattgatgg attgccgggt cgcagacaag tttacgtagt
     1081 ctgcgcaagg cagactacta tgcccggcaa tgacgaatgt ggaacccatg cctgcacgat
     1141 tattcctgtc ctccggcgat ctgatggctg accggcggtt cgattttgcg cgcgatctgc
     1201 agctgaaggg cgatcttgtc gccgccgccg atctgttgct gcaggccacc gaactcgcgc
     1261 ccggctttgc ctcggcttgg ttcacgctcg gagaaattcg tgaacggctc ggcgagcgcg
     1321 cagcggcgat cgcggccttg cgccgggcgc aggtcgcgga tcccgacgac cggcatggtg
     1381 cgagcctgcg gctgatgctg ctcggcgccg agccattgtc gtcgatgccg ctggcctatg
     1441 tgcgcgcgct gttcgatcaa tatgcgccga aattcgaagc cgctttggtg gatgatctgg
     1501 gctatcgcgg ccccgcgctg ttgttcaagg ccgtgttgtc ggtgtgcgct gctttccgta
     1561 agccggcact tttcaagcgg gcgattgatc tcggttgcgg caccgggctt gccgccaccg
     1621 cattcgcagc ccaggtcgat gagtttaccg gcatcgatct gtcgccgcgc atgatcgagc
     1681 aggcccgcgc caccgggctt tatgcgcgcc ttgaagtggc cgagatggtg gagggtttgc
     1741 gcaacaagcc tgacgccggc gccgacctca ttctcgccgc cgacgcggtg gtctacctgt
     1801 ccgatcttgc gccgctattg tgtgaagcga ggcgcgtgct ggtccgaggc ggcttgctgg
     1861 cgttcacgtc ggaaacgcat ggcggcgatt gcgtcattct tggcgagggc ctgcgctacg
     1921 cgcacagcgc cggttacctg cgcgcgtcga tcgaagcctc cggcctcgcg ctgtcgcggc
     1981 tggaagattt gtcggcccgc aacgaggaca atgcaccggt gccgggtctg gtggtggtcg
     2041 ccaaaaaaac ttgtgtctag acgctgcacc gctggcaaat ggcgacattg cgccgcaaac
     2101 cggctagttc gcacttgccg ggcgcgctgg aatgtagatc aatgtgcgat ctagggagaa
     2161 acaacaatga acaataatca atcaaggcgc gatttctgcg ccaccgctat cgcgacgatc
     2221 ctcgcgtccg ccacctccgt cagggccgcc gagaaaaaat acgatcccgg tgcgagcgat
     2281 accgaaatca agctcgggca gacggtgccg cacagcggcc cgggttcgct gtacggcgtg
     2341 cttggccggg tcggcgaagc ctatttccag atgctgaacg aaaagggcgg catcaacggc
     2401 cgccagataa aattctttac catggacgat gcctacagcg cgccgaaggc ggtcgaagcc
     2461 acgcggcggc tggtcgagca ggaggaagtg ctggccttgt tcggctcgct cggcacggcg
     2521 ccgcaaacgg cggtccataa atatctcaat tcaaagggcg tgccgcagct tctgctgaac
     2581 accggcgcat cgaagtggaa cgatccgaaa aacaacaaat ggaccatggc gggtctgccg
     2641 ctctatccga ccgaagcgcg aatcctcgcc aaatatgttt tgagcgtgaa gcctaacgcg
     2701 aagatcgcca tcctctacca gaacgacgat ttcggccgcg atttcttagg ccccttcaag
     2761 caggtgctgg cggatgccgg cggcaccgcc aaggtgatca tggagcagac ttacgatctg
     2821 accgaaccaa cggtcgattc ccagctcatc aatctctcca aatcgggcgc cgatgttttt
     2881 tacaatatct ctaccggcaa ggcggcgtcg cagtcgatcc gcaaagtggt cgaactcggc
     2941 tggaagccag tgcagttgct ttcggcggga tccaccggcc gctcgatcct cagtgccgcg
     3001 ggctttgaaa atgcggccgg gatcgttgcc atccgctaca gcaaggaagt cggcgtgccg
     3061 cgctgggcga acgatccgga cgtgatggca ttcgaggagt tgcgcaagaa atatctgccc
     3121 aatgtcgatc cggacaatac catagcgttt gccggttatg gccaggtggt gacgatggcc
     3181 gaaatcctgc gccgctgcgg cgacgatctc acccgcgcca acgttttgag acaggctgct
     3241 aacctcaccg gcttccattc gccgtttttt ctcgacggca tcacctacag ctataccccc
     3301 gacgactaca cgccgatgaa gacgctctac atatcggtct tcaacggcaa ggactgggat
     3361 atttccgacc ggccggtcac ggagtaacga gcggcgtcga tctccctggc cccgcgcttc
     3421 gcggggcgag gtgaccgagt ggccctcgac gaagcggctc tgacggcgta gctgttcagg
     3481 cgtgccgtcc cttgtcctgc ccgcgccttc ggccgaagcc gagcccccgc tgcccgaccg
     3541 cttccaaaga tggttcgcgg cgcgcggatg gtcgccgcgc gaacatcaat tggcgttgct
     3601 cgaaaaggcc cggcacgacc gttcgacact gctgattgcg ccgaccggcg ccggcaagac
     3661 gctggccggc tttctgccga cgctggtgga gctgagcgtt gctccgcgca cctctcggtt
     3721 acctctcccc ttgtggggga ggtcgacgcc cgaagggcgg cgggaggggg gtgagccaca
     3781 agcacctggc gatgcggctt acccccttcc ccaacccccg gatcaagtcc ggggcaggct
     3841 ctccaccaca aggggggagg gagttagatc gagagccctc cacaccctct acatctcgcc
     3901 gctgaaggcg ctcgcggtcg atatcgcgcg caatctggaa acgccgatcg cggaaatgaa
     3961 tctgccgatc aaggtcgaga cccgcaccgg cgatacgccg gtgtcgcggc ggcagcggca
     4021 acggcgctat ccgccggata ttctcttgac cacacccgaa caactggcgc tgctgttgtc
     4081 gtccgacgac gcgccgtttc tgttttcctc gctcaagcgc atcgtgctcg acgagctgca
     4141 cgcgctggtc acttccaagc gcggcgacct gttgtcgttg ggtctcgcgc ggctgtggca
     4201 actggcgccg cagatgcact cgatcgggct gttggccacc gtcgccgcgc cggaatcgct
     4261 ggcgcggttt ctggtgccgc agcgcgatgg cgcaagcgaa gtcgccgaca tcgtcgttgc
     4321 cggcggtgcc gcggcgcctg ttgtcgagat gctcgacacc aaggaacgcc tgccctgggc
     4381 cggccatagt gcgcgccacg cgctcaatga ggtctacgac ctgatcaagc agaacaggac
     4441 cacgctggtg ttcgtcaaca cccgcagcca ggccgagatg ctgtttcagg atctgtggcg
     4501 catgaacgac gacggccttg cgattgcgct gcatcacggc tcgctcgacg tcgcgcagcg
     4561 ccgcaaggtc gaggacgcga tggctaaggg caaactgcgc ggcgtggtct gtacctcgtc
     4621 gctcgatctc ggcgtcgact ggggcgacgt cgatctcgtg atcaatatcg gcgcgccgaa
     4681 aggcgcctcg cgcctgatgc agaggatagg ccgcgccaat caccgcatcg atgaggcctc
     4741 gcgcgcggtg ctggtgccgg ccaatcgctt cgaggtgctg gaatgcgccg tggcgatcga
     4801 cgcgatcgcc gaaaatgcgc aggacacccc gccgctccgc accggcgcgc tcgatgtgct
     4861 ggcgcagcac gtgctcggct gcgcctgcgg cgaaccgttt ctctccgacc agctctacgc
     4921 cgaagtgctg acggctgcgc cttattccag cctgacgcgg gccgatttcg acgacgtggt
     4981 cgatttcgtc gccaccggcg gctacgcgct gaaaacctac gaacgttttg cgcgcatcaa
     5041 gcaggacaaa agcgggaaat ggcgggtcgc caatccacgg acgcggcaaa gctatcgttt
     5101 gaatgtcggg accatcgtcg aagagaccat gctgaaagtc cggctggtgc gctcgcgcgc
     5161 tggtggttca ggatcgaccg gtgcgctcgg gcgcggcagc cggatgttgg ggcatatcga
     5221 ggaagccttc atcgagggcc tcgtgatcgg cgacaccttt gtctttggcg gcgaggtcgt
     5281 gcgttatgaa gcgctcgtag aggatcaggt ttacgtctcc cgggcccacg acaaggatgc
     5341 aaaagtgccg tcctatatgg gcggcaagtt tccgctatcg acgtatctgg ccgagcgcgt
     5401 ccgcaaattg ctcgacgaca agcgtgcctg gaatgcgctg ccggatcagg tgcgcgactg
     5461 gctgtcgctg cagcgggata tttcgcgggt gcccggcgtg cgcgaactgc tggtcgaaac
     5521 ttttccacgc ggcaacaagc actacctcgt ctgctatccc tttgaaggtc ggctggcgca
     5581 tcagacgctc ggcatgctgc tgacgcggcg gatggagcgc gcgcgggtgc ggccgctcgg
     5641 ctttgtcgcc aatgaatatg cgctcgcggt gtgggggctc ggcgacatgt cgttcatgat
     5701 ccggcagggc cggctcgatc tcgacgcgct gttcgatccg gacatgctgg gcgacgatct
     5761 cgaagcctgg ctggcggaat ccgcgctgat gaaacgcact tttcgaagct gcgcgatcat
     5821 ctccggattg atcgcgcggc ggtttaccgg cgaggaaaaa acccggcggc aggtgctgtt
     5881 ttcgaccgac ctgatctacg acgtgctgcg aaaacaccag ccggatcacg tgctgttgcg
     5941 cgcggcccgt gccgatgccg cgaccgggct gctcgatctt cgccggcttg gtgatatgct
     6001 gctgcgaatc aaggggcggg tcgtccacaa ggaactcgac cgggtttcgc cgctggcggt
     6061 gccggtaatg ctggaaatcg gccgtgaatc agtttatggc gaagcctccg acgagttgct
     6121 ggcggaagct gccgatgaac tggttaaaga ggcgacagga tagaccgtga cactcacgca
     6181 atatatcgac gaacagcctg ttgcgcgcgt taccagcgtc gacgtcgcgg gcgtgactct
     6241 ggtcgccgat ctctccggcg cgttgttctg ggaagagcaa agcctgctcg tcgtctccga
     6301 cctgcatctg gaaaaaggct ccagttttgc cgcgcgcggc gtgctgctgc cgccttacga
     6361 taccgtggcg acgctcgggc gcctcgctgg cgcgattgtg cggcacgacc cgcgcatggt
     6421 gatcgcgctc ggcgacagtt ttcacgaccg caccgcccat gaccggttgt ccgcaagaga
     6481 tcgcgacgcc ctctcggcat tgcaggcgag gcgcgactgg atctggattt ccggcaatca
     6541 cgatcccgcg ctcccctgcg atctcggcgg cgtggtcgcc agcgaagtcg ccgtcgggcc
     6601 gatcgcgttt cgtcacgagc cgacaggcgc cgccggtgaa atcgccggac atctgcatcc
     6661 caaggcgcgg gtgtcgacgc gcggtcgttc gatggagcgg cgctgttttg cctgcgacgg
     6721 cgagcgcgcc gtgatgccgg cgttcggcgc ctataccggg ggtttgagca ttcgcgatgt
     6781 ggcgtttgca aaaatattcc agaccccggg cttgatggcg catgtgctcg gcgatcacag
     6841 gctacacacc atcgccgcgt cgcgctgcta ctgatcgcga tttaagccgc cttcgcctgc
     6901 accgtattgc agaactcggc cagacgatcg gccagccgca atgttgcggg gctggcgtcg
     6961 ggtgaggcca tcagcgccac ttccgtcttg tcgatcggcg caaaaccatc ctttgcggtc
     7021 agcacgcgat gctcggcctg gatcgccatc tcggaaagaa tgcttaagcc caatccggcg
     7081 gcgaccgcgg cctggatgcc ggcgaggcct gaactggcat aggccatgtg ccaggcgcgc
     7141 ccggcgcttt ccagcgcgtg gatcgcccgc gcccgataca ggcagccggg gggaaatccg
     7201 atcaagggca cggacccgct tgttgcgtcg accggatggc tcttgctggt gacccagtgc
     7261 acgcgctccg gccacaccgc gatgccgccc ttttctccgg catcgcgctt gagcaacgcc
     7321 agatcgagtt cgccgcgttc gagatcgcgc cgcaaataaa cgctctgatc ggcgcgcacg
     7381 tcgagccgca aaccgggccg cgcccgggta aacgtcgcca gcaattttgc cagccgatag
     7441 gcggcgaaat cttcgggaat gccgagccgc accgcgcctt cattgcccgg ccgtgtcatg
     7501 acatcgcggg cttcctccgc cagcgacaac agccgccgcg cataggacag cagccgttcg
     7561 ccggcctcgg tcggcgtcac gtccttggcc gtgcggttga gcagcggctg gccgagatcg
     7621 tcctccagcc gcttgatctg ctggctgacg gtggactggg tgcggtgaac gcgttcgccg
     7681 gcgcgggtga agccgccgga atcgaccacc gaaacaaagc tgcgcagcag ttcgagatcg
     7741 agcatgaagg ccaccattca tatttccact ggatgacagt ttatcattta atttccaaat
     7801 atcaagccgc ggacctacat cagggaacaa ggagaatttc catgtccgtt gccccatccg
     7861 ttgcgcttcc ccgcgccgcc ttcaacccgt tgccgatcta tatcgccctg ttctgcctgc
     7921 tctggagctt tgccttcgtt gcaagcaaga tcgcggtcac cgattgcccg ccgctcatcc
     7981 tgctcacggc gcggttttcg ttggcgggaa tactggtgct cggaatctcg gcgttgcgcg
     8041 gcgacccctg gtcgctgtca ccgcgcgata cctgcgggtt cgcgatcctc ggcgtcgcca
     8101 acaacgcgct ttacctcggc ctcagctata ccgggttgca gtccgtgtca gccggccttg
     8161 ccggcctgat cgtcagcacc aatccggtgt tcaccgcggt gctggcggcg ttgcttctga
     8221 aggaagcgtt gagctggcgc aagatcgcgg ggctgctgct cggcgttgcc ggggtgagct
     8281 ttattgtctg gcaccggatc tcggtcggcg ctgatagctg gcacggaatc ctgttcatgc
     8341 tcgggtcgct ggcgtcgatt gtagcaggca cccttctgtt caagctgctg gccccgaagg
     8401 gcagcctgtg gatcggcacc ggcgtccaga atctgacggg tggtatcgtc ctcctcccat
     8461 tcgcattcac cctctcgagc gtaggcgaca tcgtaccgag cgtccggctt ctgggagcat
     8521 tcgcattcct ggtgctgggc ggttcgatcc tcgcctattt cctatggttt catctgctca
     8581 acgtatgcgg agcgaccgcc gccagcgcct atcacttctt gatgcccccg cttggcatgc
     8641 tgttcgcctg gattattctt ggcgagcacg tcgaagtcag ggatttgatg gggatcgttc
     8701 cagtggcgct cggtatttat ctggtgacgc gtcccgccgc ggcgtcggcg ccatagagag
     8761 ggaggtccac atgaatccgc ttcgtatcac gctgatcggc ggccccaccg cgctgattga
     8821 gatcgacggt tttcggatcc tcaccgatcc gaccttcgat gcgcccgggg cctatcaatt
     8881 gccgcatgtg aagct
//
LOCUS       DASWGM010000698         5403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2688594,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000698 DASWGM010000000
VERSION     DASWGM010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2688594"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VGA15_19650"
     CDS             <1..240
                     /locus_tag="VGA15_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9589948.1"
                     /translation="CVPDAELEKATDKLVDELRTFSPLAQRTAKKLLNDTEDSTLAIA
                     IELEGHCYSRLRQSADFKEGVEAFNAKRAPNFTGL"
     gene            408..1682
                     /locus_tag="VGA15_19655"
     CDS             408..1682
                     /locus_tag="VGA15_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9589949.1"
                     /translation="MINAIIASLLGWSLDLFDLFILLYVAPVIGVLFFPSTNATLSLA
                     AVYASFAVTLLMRPIGSAIFGHFADVHGRKGAMLVAIVGVGVTTAAFGALPTIAKVGI
                     AAPIMFLILRLVQGIFVGGVVASTHTIGTESVPATWRGAMSGLVGGGGAGIGALLASF
                     VFLIASSVFPGEAFAQWGWRFMFFSGLLSSLLGWFIFKNLEESPFFNELQQQKAARKA
                     MAAPSPVRVLFSSDYRSVLLINLLITFGGGAGYYLTSGYLPSFLKVVNGVPNNVSSLI
                     LMGASVAAFISAVLFGALSELIGRKPTFLIVGVCALVWLPLCYLGLAKATDIKEVTIY
                     ALAIAFIGNAGFAPVLVFLNERFPTSLRASGTGLSWNIGFALGGMMPTFVSLASAGTA
                     QIPTSLAIFSVAIYLIYLIGALIIPETRGNFR"
     gene            1996..2514
                     /locus_tag="VGA15_19660"
     CDS             1996..2514
                     /locus_tag="VGA15_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589950.1"
                     /translation="MIRTMWLTIACLAVLAALLVGKALKTPVALAAVTPIDETTIGAG
                     SAQDPLTKADRLEKADRLEFARVRPEIPAQSALRPTEPLIPPVPTSVPPVETKIVSRD
                     WHDPDVTASLAGKSKKPTRMAAGKKGKSVDAKGSRMADRSRPARPAKPCHRTGAFDDF
                     LKSLKLSPACVS"
     gene            complement(2628..3194)
                     /locus_tag="VGA15_19665"
     CDS             complement(2628..3194)
                     /locus_tag="VGA15_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589951.1"
                     /translation="MGWLFAVALGLHRQSRASVLLALPPIAFGHAVSIAMVAGAFVAA
                     GVLMNQRAIQVSAGLLLIGWSVYHQLYGHRHRVRVGMKTGQAGLVLWSFLMATAHGAG
                     FMILPAMIPLCLASSSAGETGASGPAATALAAVAVHTAAMLVVTGAVAVIVYEWVGLS
                     ILRTAWLNPDLLWTGALAGTGLVLLLLA"
     gene            complement(3307..4701)
                     /locus_tag="VGA15_19670"
     CDS             complement(3307..4701)
                     /locus_tag="VGA15_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding family protein"
                     /protein_id="HEX9589952.1"
                     /translation="MATWKPDPTFYPSPRMAMKAPAEKLAYVAAFDPSRKAPDGIAVV
                     DLDPDSKHYSTILNTVAMPNVGDELHHFGWNACSSCLCPNAPHPHVERRYLVVPGLRS
                     SRIHILDTKADPTKPTIVRVIEPEEVAEKAGYTRPHTIHCGPDGIYVAALGNAEGKAP
                     GGIFLMDHESFDILGRWEIDRGPQQLAYDAWWHLGHDTLVTSEWGTPDTFENGLVPEI
                     LLGAKYGRKLHFWDLHTRKHRQTIDFGDKYQLVFELRPAHDPTKAYGFVNCVISLEDL
                     SSSIWVWHRDGEKWGVTKVIDIPAEPADPDQLPPLLKGFKAVPPLVTDIDLSMDDKFL
                     YVACWGTGDLRQYDVSDPFKPKLTGKVRIGGIVSRATHPGAKNGALSGGPQMVEISRD
                     GRRVYFTNSLYGAIDEQFYPDGIEGWMVKLDAKPEGGISFDEKFFVEWPKSHRPHQVR
                     LEGGDCSSDSYCYP"
BASE COUNT         1030 a         1623 c         1656 g         1094 t
ORIGIN      
        1 tgcgtgcccg atgccgaatt ggagaaagcg accgacaagc ttgtcgacga acttcgcacg
       61 ttctcgccgc tggctcagcg aaccgccaaa aagctcttga acgacaccga ggactcgacg
      121 ctcgcgattg cgatcgagct cgagggccat tgttacagcc gcctgcgtca atcggcggat
      181 ttcaaggaag gcgtcgaggc cttcaacgcc aagcgcgctc cgaactttac gggactatga
      241 ccgatgccgg atggggtccg gcacacacga catactgggt cggtccggat ggtccggcgg
      301 cccggtcgta tgctgctgag ttgcgagaac cacaaaagag gggcttcgaa atgacaatga
      361 caagtgcgcc gttggcggat gcgcaggcac ctgccgataa ccgacaaatg atcaatgcga
      421 ttatcgcctc attgctgggc tggtcgctcg atctgttcga tctgtttatt ctgctctatg
      481 tcgcgccggt cattggagtt ctgtttttcc cttccaccaa tgcgacgctg tcgcttgcag
      541 cggtatatgc atcgtttgcg gtgacgctgc tgatgcgccc catcggctcg gcgatattcg
      601 gccatttcgc cgatgtccat ggtcgaaagg gcgccatgct ggtcgcgatc gtcggcgtcg
      661 gcgtcactac cgcggccttc ggtgcgctgc cgacgatcgc gaaggtcggc atagccgcac
      721 ccatcatgtt cctgatcctc cgtctggttc agggcatatt cgtcggcggc gttgttgcgt
      781 cgacgcatac cattggaact gaatcggttc ccgcgacctg gcgcggggcg atgtcgggcc
      841 tagtgggcgg cggcggcgcg ggcatcggtg cgttgctggc gtcgttcgta ttcctgatcg
      901 catcgtcggt attccccggc gaggcctttg cgcaatgggg atggaggttc atgttcttct
      961 cgggccttct gagttcgttg ctcggatggt tcattttcaa gaacctcgag gagtcgccgt
     1021 tcttcaacga gttgcagcaa cagaaggctg cgcgaaaggc gatggcggca ccctcgccgg
     1081 tcagggtcct gttttcgagc gattaccgct ccgtgctgct gatcaacctc ttgattacat
     1141 tcggcggcgg cgcgggctac tacctgacgt caggctatct cccgagcttc ttgaaggtcg
     1201 tcaacggcgt tccgaacaac gtctcgtcgc tgatcctgat gggagcgagc gtggcggcgt
     1261 tcatctccgc cgtcctgttc ggggcgctga gcgaattgat cgggcgcaag ccgacatttc
     1321 ttatcgtcgg cgtctgtgcg ctggtgtggt tgccgctgtg ctatctcggc ctggccaagg
     1381 caaccgatat caaggaagtg acgatctatg cactggccat cgcattcatc ggcaacgccg
     1441 gctttgcccc ggtcctggta tttctcaacg agaggtttcc gacctcgttg cgtgcgagcg
     1501 gcacgggatt atcctggaac atcggcttcg ccctgggtgg catgatgccg accttcgtct
     1561 cgctcgcaag cgccggcacg gcgcagatcc cgacgtcgct tgcgatattt tcggtggcga
     1621 tctacctgat ctacctgatc ggcgcactga tcattccgga aaccaggggc aattttcgtt
     1681 gactttgtcc gggtgttgaa gcgcgaggcg ccgggattgc aagatgccgg cgcgagataa
     1741 cacataccga aagacccgcg ctttccaaca acagacggtc aatgcgaccc tgacaatgcg
     1801 accctgaaat ttcccgatgg atgggactca tttcaggcga ggtcgtacgg gcaaaggcgc
     1861 cctcgacgag ggctgccggc cagaatttgg cggataatcc cgaggccgtg tccacaagca
     1921 agatctagct tgccctctta ccgcccgaat cgctccggaa cttgcggcag acccatgggt
     1981 ctgccggatt gaaccatgat caggacaatg tggcttacga ttgcgtgttt agccgtgctc
     2041 gccgccttgc tcgtcggcaa ggctctcaaa acacctgtcg ctctggcagc ggtgacaccg
     2101 atcgacgaga cgacaatcgg cgccggttca gctcaagatc cattgaccaa ggctgatcgg
     2161 cttgaaaagg ctgatcggct tgaatttgcc cgtgtgcgtc cggaaatccc ggcccagtcc
     2221 gcattgcggc cgaccgagcc actgattccg cccgttccaa caagcgtccc gccggtcgag
     2281 accaagatcg tcagccgaga ttggcacgat ccagacgtta cagcttcatt ggccgggaag
     2341 tccaaaaaac ccacgcgaat ggctgctgga aagaaaggca aatccgtcga tgccaagggc
     2401 agtcggatgg cggatcgttc caggccagct cgacctgcaa aaccctgtca ccggaccggc
     2461 gcctttgatg attttctgaa gtcattgaaa ctgtcgccgg cctgcgtctc atagtttttc
     2521 cgcattcgtc acaggtggcc tacgctcccg gatctcttgg ctcgacctcg cacgcctctg
     2581 actattctgc gctggcgctg cgatcgcgca gcgggaccgc gtttcggcta cgcgagcaaa
     2641 agcaacacca gcccagtgcc ggcgagcgct ccggtccaga gcaggtcggg gttgagccat
     2701 gcggtgcgca atattgaaag accaacccat tcgtagacaa tgaccgcgac cgccccggtg
     2761 actaccagca tcgccgccgt gtggaccgca acggcggcaa gcgcagttgc ggcgggcccg
     2821 cttgcccctg tctcgcccgc tgatgaggat gcaaggcaga gtgggatcat cgctggcagg
     2881 atcatgaatc ccgcaccatg cgctgtcgcc atgaggaacg accaaagaac cagcccagcc
     2941 tgccccgtct tcataccgac gcgtacacgg tgccggtggc cgtaaagttg gtggtaaacg
     3001 gaccatccga tcagcagaag accggccgag acttgtatcg cgcgttggtt catgagaacg
     3061 cctgccgcga cgaaggctcc ggccaccatc gcgatcgaaa ctgcgtggcc gaaggcaatt
     3121 ggcggcagcg ccaacaggac agaggctcgg ctctgtcgat gcagtccgag cgccacggcg
     3181 aacagccagc ccatggccgg gtttagccca tggaaggcgc cgagcccggt caaagccagc
     3241 cagggccagg ggccggtcat tgagcgtgcc cccccttggc ggacaagccg gcggccgaac
     3301 gcgcgatcac ggatagcaat agctatcgga cgagcagtcg ccgccttcga ggcgaacctg
     3361 gtgcgggcga tgtgatttcg gccattcaac gaagaacttc tcgtcgaagc tgatgccgcc
     3421 ctctggcttg gcgtcgagct tcaccatcca gccttcgatg ccatcggggt agaactgctc
     3481 gtcaatggcg ccatagagcg agttggtgaa atagacccgg cgaccatcgc ggctgatctc
     3541 caccatctgc gggccgccac tcagggcgcc gttcttggca ccggggtgcg tcgcgcgaga
     3601 tacgatgccg ccgatccgga ccttgcccgt caatttcggc ttgaagggat ctgaaacgtc
     3661 gtattggcgc agatcacccg tgccccagca cgcgacgtac aagaacttgt cgtccatgga
     3721 gagatctatg tcggtaacga gtggtggcac cgccttgaag cctttgagga ggggaggcag
     3781 ctgatcgggg tcggcgggtt cggccgggat gtcgattacc ttggtaaccc cccacttctc
     3841 gccgtcacga tgccaaaccc aaattgagga agagaggtct tccaggctta tgacgcagtt
     3901 gacgaaccca tacgcctttg tcgggtcatg agccggacga agctcgaaca cgagctggta
     3961 tttgtcaccg aagtcgatgg tctgccgatg cttgcgggtg tgtaggtccc agaagtgcag
     4021 cttacggccg tactttgcgc ccaaaagaat ttcgggaacg agtccgtttt cgaaagtgtc
     4081 gggcgtgccc cattcgctgg tgacgagtgt atcgtggccg aggtgccacc acgcatcata
     4141 cgcgagctgc tgtgggccgc ggtcgatctc ccagcgcccg aggatgtcaa agctttcgtg
     4201 atccatcagg aagatgccgc cgggcgcctt gccttccgcg ttgccgaggg ccgcaacata
     4261 aattccatcc ggaccgcaat ggatcgtgtg tggccgcgta taaccggcct tctccgcaac
     4321 ctcttccggc tcgatcaccc tgacgatagt tggtttcgtc gggtcggcct tggtatcgag
     4381 gatgtgaatg cgcgatgacc tcaggccggg tacgaccaga tagcgccgct cgacatgtgg
     4441 gtgaggtgca ttcgggcaga gacaggacga acacgcgttc caaccgaagt ggtgcaactc
     4501 gtcgccgaca ttcggcattg cgaccgtgtt taggatcgtt gagtagtgtt tcgagtccgg
     4561 gtcgaggtcc acgacggcga tgccgtcggg ggccttacgt gaaggatcaa acgccgcgac
     4621 ataagccagc ttctcggccg gcgccttcat ggccatgcgg ggtgagggat agaaagtcgg
     4681 atcgggtttc caggtggcca tgatgtgttc tcctctcgtt gcggatcctt acttctctgc
     4741 tcaggcggcg attacgcact ccccgagtaa gctcgcgctg cagatcggcg tcctgcggca
     4801 gcgccgggcg cactgtgatg cggcttcggt tccccagctg caccacatcg atcaggtgta
     4861 ccggatagcg ctggaaggtg caggttatca cacgacctcg acagcagcgt acacatcgcg
     4921 gcgagcggca ccgggcggcc tgcagttccc tcgtcccggc ggcgatccgg catccacctc
     4981 cacgacgtgg cgggcgatcg ccgagctgac ggcacccacg tgcgtcagcg gacccatgta
     5041 acctgcgccg tcgatcacgg ccaggtttcc gaatcgactt tgaccagccg catcaatgcc
     5101 gcccggcgtg cgatcggcga cagtggcgcc aacctgcagg atccgacgag gcagacccga
     5161 tgatcccgga aacagaggtc ctttcagagt tgatcggaac tatctatgac atgacgatca
     5221 accgcgcctt gatgcgcggg ttcggcgcga ggtcagcccc ctcgatttgg attggctttg
     5281 gcgccgtaat cgcgatattt ctgaacgatc gcggcgaccg ctgggcggga caaagacaaa
     5341 gatgaaacga tgaatggtac ggcccgcttt cgcgcccgtg atcgatcttc cgaaactctc
     5401 gaa
//
LOCUS       DASWGM010000699         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2688904,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000699 DASWGM010000000
VERSION     DASWGM010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2688904"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..865
                     /locus_tag="VGA15_19675"
     CDS             35..865
                     /locus_tag="VGA15_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX9589953.1"
                     /translation="MAKKKRGDAHGGGHGWFVTFADLMGLMMSFFVMLVAFSTMDNNK
                     LKIVAGSMREAFGVQTEARYSGIIESDGLPTRPKLKNTAHIPPEEASNTPTPDEQERN
                     KTTGARLKIDREFALASASLRQALQDMPELTEISKHVMFEETNEGLNLEIVDQDGRSM
                     FADGSKVPNDRTRRLIQKLAAPLRATPLRVSIVGHTASGFLPTQSDYGAFDLSADRAN
                     AVRQILEREGLPPAHIFAVSGRADSQPLFPDDPSLAANRRVTITLMREDPALPPDLKP
                     "
     gene            1028..>2113
                     /locus_tag="VGA15_19680"
     CDS             1028..>2113
                     /locus_tag="VGA15_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX9589954.1"
                     /translation="MAVSVTSTEARDGPVTSGFWALTLGSIGVVFGDIGTSPLYAFRE
                     AVAGASQGQPATPVIVLGVLSLILWALFIVVTAKYVLLLLRADNNGEGGTLSLMALGQ
                     RALGRRSWPLLALGVIGASMFIGDSMITPAISVLSAVEGLKLAAPALEHYVVPLTVFI
                     LVVLFSVQSSGTARVASAFGPVMIVWFATLAVMGLVHISDDPSVLAAINPWYGIHFLL
                     SHGTIGMVTLGAVFLAVTGGEALYADLGHFGRKPIQAGWLYFVLPSLLINYFGQGALV
                     LADPAAIENSFYRMVPEILLLPLVVLATAATVIASQAVITGAYSLTRQAVQLGLLPRF
                     EVRYTSESHAGQIYLPRVNRLLLIGVLL"
BASE COUNT          365 a          637 c          659 g          452 t
ORIGIN      
        1 cgtaaacggc tgaccacggt tcggaaattc aacgatggcc aagaagaaac gtggcgacgc
       61 gcatggcgga gggcacggct ggttcgtgac ctttgccgac ctcatgggtc tgatgatgag
      121 cttcttcgtg atgctcgtcg cgttctccac catggacaac aacaagctca agattgtcgc
      181 cggctcgatg cgcgaggctt tcggcgtgca gaccgaggcg cgttattccg gaattatcga
      241 atccgacggc ctgccgacac ggccgaagct gaaaaacacc gcgcacattc ccccggagga
      301 agcatccaac acgccgaccc cggacgaaca ggagcggaac aagaccaccg gcgcccgact
      361 gaagatcgat cgcgagttcg cgctcgcttc ggcatcattg cggcaggcgt tgcaggacat
      421 gccggagctg accgaaatct caaaacacgt catgtttgag gagaccaatg agggtctcaa
      481 cctggaaatc gtcgaccagg atggccggtc gatgtttgcc gacggctcca aagtgccgaa
      541 tgaccgcacc cgccggctga ttcagaaact cgcagctccg ctcagggcga cgccgctccg
      601 cgtttccatc gtcggtcaca ccgcgtcagg ctttttgccg acgcagagcg actacggcgc
      661 gttcgatctc tcggcggatc gtgccaacgc ggtgcgccag attctggagc gggaagggct
      721 accgccggcc catattttcg cggtctctgg aagagcggac agccagccgc tgtttcccga
      781 cgatccgtca ttggcggcga accgtcgcgt caccatcaca ttgatgcgcg aggatccggc
      841 gctgccgccc gatctgaagc cgtaattcaa ctacactacc atgttaccag agactgttct
      901 gatgaagccg cttcacgggc gatcgcggca tcgccggtga tgctatggtt gatcggttga
      961 caggctttcc agaagcgtcg atagccggcc cggatccaat caaccgatag agcgtttttc
     1021 cagcatcatg gctgtcagcg tcacatcaac cgaagcccgc gacgggccgg ttacatcagg
     1081 cttttgggcg ctgacgctgg gaagtatcgg cgtggtgttc ggcgatatcg gaacctcgcc
     1141 tttgtacgcg tttcgcgaag cggtcgccgg tgcttcgcag ggccaaccgg caacgccagt
     1201 cattgtgctt ggggttttgt cgctgatcct gtgggcgctt tttatcgtcg tcaccgcgaa
     1261 atacgttctg ctgctgcttc gcgccgacaa taacggcgag ggcggcacgc tgtcgctgat
     1321 ggcgctgggt caacgcgcgc tggggcggag aagctggccg ttgctggcgc tcggcgtcat
     1381 cggcgcttcg atgttcatcg gcgattccat gatcacgccg gcgatttcgg tcctgtccgc
     1441 ggtggaaggc ctcaagctcg cggcgccggc gctcgaacac tatgtggtgc cgctgacggt
     1501 gttcatcctc gtcgtgctgt tctcggtcca gagcagcggt accgctcgcg tcgcatcggc
     1561 ttttgggccg gtcatgatcg tctggttcgc tacgctggcg gtgatgggcc tcgttcatat
     1621 cagtgacgat ccgtcggtgc tggccgcgat caatccctgg tacggcattc atttcctgct
     1681 ctcccacggc acgatcggaa tggtgacgct gggagccgta tttctcgcgg tgaccggcgg
     1741 cgaggcgctc tatgcggatt tgggtcactt cggacgcaag ccgatccagg ccggctggct
     1801 ttatttcgtg ctgccttcgc tgttgatcaa ctatttcggt cagggcgcgc tggtgcttgc
     1861 cgatcccgcg gcgattgaga attccttcta tcggatggtt cccgagatat tgctgttgcc
     1921 gctggtggtg ctggcaaccg ccgcgaccgt gatcgcgagc caggcggtga tcaccggagc
     1981 gtattcgctg acccgtcagg ccgtgcagct tggcctgctg ccgcgcttcg aggtccgcta
     2041 cacctccgag tcgcacgccg gacagattta tttgccgcgc gtgaaccggc tgttgctgat
     2101 cggcgtcttg ctg
//
LOCUS       DASWGM010000700         5176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2448363,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000700 DASWGM010000000
VERSION     DASWGM010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448363"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="VGA15_19685"
     CDS             <1..1698
                     /locus_tag="VGA15_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX9589955.1"
                     /translation="SMLLWVSILALFGGLVAAFGNNLPLSLRAHVLAVQAWIASAFYL
                     FILITSNPFLRIDNPPIEGRDLNPVLQDIGLAVHPPMLYLGYVGFSISFSFAVAALLE
                     GRIDAAWARWVRPWTLVAWIFLTLGIAMGSYWAYYELGWGGWWFWDPVENASVMPWLA
                     GTALLHSAVVMEKRNALKVWTILLAILTFSLSLLGTFLVRSGVLTSVHAFATDPTRGV
                     FILLILCIFIGGSLTLYAWRASALKQGGLFAPISREGALVLNNLFLTTACATVFIGTL
                     YPLALEVLTGDKISVGAPFFNLTFAPLFLPLMIAVPFGPLLAWKRGDLLGAAQRLIAA
                     GIAALVAVAVLWAWTRGGSAFAPLAIGLAVFVISGALCDLAERTGLFRMPFATALRRA
                     RGLPRSTWGTMFAHAGVGIALIGIVCETTWNSEYVGSMKPDDVAKIAGYELKLDGVTQ
                     RQGPNFREMLAQFTVTLDGKPLSAMTPSKRNFPTRGSSTTEAALLTRGASQLYISLGD
                     TTADGAIAVRIYHKPLVLLIWGPVLMAFGGMLSLSDRRLRVGAPKPAKALRGLQAAE"
     gene            1703..2200
                     /locus_tag="VGA15_19690"
     CDS             1703..2200
                     /locus_tag="VGA15_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX9589956.1"
                     /translation="MKPILACAFAIAVMMGSSAAYAVQPDEIMSDPAMETRARDLSRE
                     LRCMVCQNQSIDDSEAPLARDLRLLVRERIAAGDSDAQVIDFLVARYGEFVLLKPRLK
                     PHTLLLWLLPPLALAGGGFALWTYGRRSRFAAAEDQSPLELTAEEEARLERLTAAEPP
                     ADKPA"
     gene            2422..4020
                     /locus_tag="VGA15_19695"
     CDS             2422..4020
                     /locus_tag="VGA15_19695"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX9589957.1"
                     /translation="MTDRPTDLSSLPSYRTPRRSLFSARKFALMASVVAGLGVAVYGF
                     SPSPGPVDIFSSPAHAQVNNEVRKVEKPVGFADIVERVKPSVISVKVNINEKLAKDDS
                     ANNNDDSPFQPGSPMERFFRRFGGPDGMPPGLRGGPRGRGAVTGQGSGFFISADGYAV
                     TNNHVVDGADKVEVTTDDGKTYTAKVIGTDARTDVALIKVEGGSNFPFAKLSDGKPRI
                     GDWVLAVGNPFGLGGTVTAGIVSASGRDIGNGPYDDFIQIDAPVNKGNSGGPAFNTEG
                     EVMGVNTAIYSPSGGSVGIAFSIPASTVKNVIAQLKDKGSVSRGWIGVQIQPVTADIA
                     DSLGLKKAEGALVAEPQANGPAAKAGIESGDVITAVNGDAVKDARELARTIGALAPGN
                     AVKLNVLHKGQDKVVNLTLGQLPNTLEAKADNDNDNGDKGSATRGTDVPKLGLTLAPA
                     NSVAGAGKDGVVVTEVDPKSAAAERGFKEGDVILEVAGKSVANAGDVREAINAARTDN
                     KNSVLMRVKSGGSLHFVAVPLAKG"
     gene            4296..4976
                     /locus_tag="VGA15_19700"
     CDS             4296..4976
                     /locus_tag="VGA15_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9589958.1"
                     /translation="MRLLIIEDDRESADYLVKAFREVGYVADLASDGEEGLSLADSGD
                     YDVLVIDRMLPKRDGLSLIGTLREKGNRTPALILSALGQVDDRIKGLRAGGDDYLPKP
                     YSFAELLARVEVLSRRHGGPAEETTYRVGDLELDRLSHRVARGKDELTLQPREFRLLE
                     YLMKHAGQVVTRTMLLENVWDYHFDPQTNVIDVHISRLRSKIDKGFERPLLHTIRGAG
                     YMIRDGIR"
BASE COUNT          896 a         1639 c         1649 g          992 t
ORIGIN      
        1 tcgatgctgc tgtgggtgtc gatcctggca ctgttcggcg gcctcgtcgc ggcgttcggc
       61 aacaatctgc cgctgtcgct gcgtgcccac gtgctggcgg tgcaggcctg gattgccagc
      121 gcgttctatc tgttcatcct catcacttcg aacccgttcc tgcgcatcga caatccgccg
      181 atcgagggcc gcgacctcaa tccggtgctg caggatatcg gcctcgcggt gcatccgccg
      241 atgctctatc tcggctacgt cggattctcg atttcgtttt cgttcgccgt cgcggccctg
      301 ctggagggcc ggatcgacgc ggcgtgggcg cgttgggtgc ggccgtggac gctggtggca
      361 tggatattcc ttacgctcgg catcgcgatg ggctcgtact gggcctatta cgaactcggc
      421 tggggcggct ggtggttctg ggatccggtc gagaatgcct cggtgatgcc gtggctcgcc
      481 ggtaccgcgc tgctgcattc cgctgtcgtg atggaaaagc gcaacgcgct caaggtctgg
      541 accatcctgc ttgcgatcct gactttctcg ctgtcgttgc tcggcacctt cctggtgcgc
      601 tcgggcgtgc tgacgtcggt gcatgcgttc gcgacggatc cgacgcgcgg cgtgttcatc
      661 ctgctgatcc tctgcatttt catcggcggc agcttgacgc tgtatgcgtg gcgggcctcg
      721 gcgctgaagc aaggcgggct gttcgcgccg atctcgcggg aaggcgcgct ggtgctcaac
      781 aatctgttcc tcaccacggc ttgcgcgacg gtgtttatcg gaaccttgta tccgctagcg
      841 ctggaagtcc tgaccggcga caagatttcg gtcggtgcgc cgttcttcaa tctcaccttc
      901 gcgccgctgt tccttccgtt gatgatcgcg gtgccgttcg ggccgttgct ggcgtggaag
      961 cgcggcgacc tcttgggtgc ggcacagcgc ctgatcgccg ccggcatcgc ggcgctggtt
     1021 gccgtcgcgg tgctatgggc gtggacccgt ggcggcagcg ccttcgcgcc gctggcgatt
     1081 gggcttgccg tcttcgtcat ctcgggcgcg ctttgcgacc tcgccgagcg caccggcctg
     1141 tttcgtatgc catttgcaac cgccctgcgg cgcgcgcgcg gattgccgcg ttcgacctgg
     1201 ggcacgatgt tcgcccatgc cggcgtcggc atcgctctga tcggaattgt ctgcgagacc
     1261 acgtggaaca gcgagtatgt cggatcgatg aagcccgatg acgtcgccaa aatcgccggc
     1321 tatgaattga aactcgacgg cgtgacgcag cggcaggggc cgaattttcg tgaaatgctg
     1381 gcgcagttca ccgtcacgct cgatggcaaa ccgctcagcg cgatgacacc gtcgaagcgc
     1441 aattttccca cgcggggatc atccaccacc gaggcggcgc tgctgacgcg cggcgcaagc
     1501 cagctctata tctcgctcgg cgataccaca gccgatggcg ccatcgcggt gcgtatctat
     1561 cataagccgc tggtgctgct gatctgggga cctgtgctga tggcgttcgg cggcatgctg
     1621 tcgctgtcgg atcgccgctt gcgggtcggc gcgccgaagc ccgccaaagc gctgcgcggc
     1681 ctgcaggcgg cggagtgatc aaatgaagcc gatccttgcc tgcgcctttg ccatagccgt
     1741 gatgatggga tcgtcggccg cctacgccgt gcaacctgac gagatcatgt cggatccggc
     1801 aatggaaacg cgggcgcggg acttgtcgcg cgaattgcgc tgcatggtgt gccagaacca
     1861 gtcgatcgac gattccgaag cgccgctggc gcgcgatttg cggctcctgg tgcgcgagcg
     1921 gatcgcggcc ggcgacagcg atgcgcaggt gatcgatttc ctggtggcgc gctatggcga
     1981 gttcgtgctg ctgaaaccgc ggctcaagcc gcacaccttg ttgctgtggc tgttgccacc
     2041 gctggcgcta gccggcggcg gatttgcact gtggacgtat ggccggcgct ccagatttgc
     2101 ggcagcggaa gatcaatcgc cgctcgaatt gaccgcggaa gaagaagcgc ggctggaacg
     2161 gctgaccgca gcggaaccgc cggccgacaa gccggcctag gctcgcgata aaccgttttc
     2221 gcccggcgat aggccctttc tcaccctgcg ataaatcgct ttcgccctgc gataaactgc
     2281 cgcattgcca tccggcttca ttaccaaagt ttaattccca agccagcgcg cggtaaggcg
     2341 gaagacccca tcttggggcc tgtaaggtgc ttgcgcctgc gcaccgtcga tttcacgctc
     2401 acacgctttg gagacctcta aatgaccgac cgtcccaccg atctttcgtc gctaccttct
     2461 taccggacgc cacgccgttc gctgttctcc gcacgcaaat tcgcgctgat ggcctcggtt
     2521 gtcgccggcc tcggcgtcgc ggtttacggc ttcagcccct cgcctggccc ggtagatatt
     2581 ttctcaagcc ccgcgcacgc gcaggtcaac aacgaagtcc gcaaagtaga gaagcccgtc
     2641 ggctttgccg acattgtcga gcgcgtgaaa ccgtcggtga tctcggtgaa ggtcaacatc
     2701 aacgagaagc tcgccaagga cgatagcgcc aacaacaatg atgactcgcc gttccagccg
     2761 ggctcgccga tggagcgttt cttccgccgc ttcggcggac ctgacggaat gccgccgggc
     2821 ctgcgcggcg gaccgcgcgg ccgaggtgct gtgaccggtc aaggctccgg cttcttcatc
     2881 tccgccgacg gctatgccgt caccaacaat cacgtggtcg acggcgccga caaggtcgag
     2941 gtcactaccg acgacggcaa gacctacacc gcgaaagtga tcggcaccga cgcacgcacc
     3001 gacgtcgcgc tgatcaaggt cgagggcggc tcgaatttcc cgttcgccaa gctttcggac
     3061 ggcaagcccc gcatcggcga ctgggtcctc gcggtcggta atccgttcgg tctgggcggc
     3121 accgtgactg ccggcatcgt ctcggccagc ggccgcgaca tcggcaacgg cccgtacgac
     3181 gatttcatcc agatcgacgc gcccgtgaac aagggcaatt ccggtggccc agccttcaac
     3241 accgagggcg aagtgatggg cgtcaacacc gcgatctact cgccgtccgg cggcagcgtc
     3301 ggcatcgcgt tctcgatccc ggcctcgacc gtgaagaatg tgatcgccca gctcaaggac
     3361 aagggctcgg tcagccgcgg ctggatcggg gtccagattc agcctgtcac ggctgacatc
     3421 gccgacagcc ttggcctcaa aaaggccgaa ggcgccctgg ttgccgaacc ccaggccaac
     3481 ggtccggcgg caaaggccgg catcgaatcc ggcgacgtga tcacggcggt gaatggtgac
     3541 gcggtcaagg acgcccgcga actcgcccgc accatcggcg ctctcgcgcc cggtaatgcg
     3601 gtgaagctca acgtccttca caagggccag gacaaggtcg tcaaccttac cctcggccag
     3661 ctgccgaata cgctggaagc caaggctgac aacgacaacg acaacggcga caagggcagc
     3721 gcaacccgcg gtaccgacgt gccgaagctc ggcctgacgc tcgcgcccgc caacagcgtt
     3781 gccggcgccg gcaaggatgg cgtcgtggtg accgaggtcg atccgaagag tgcggcggcc
     3841 gagcgtggct tcaaggaagg cgacgtgatt ctcgaagtcg cgggcaagag cgttgccaat
     3901 gccggagatg tgcgcgaagc gatcaacgcc gcgcgcaccg acaacaagaa cagcgttctg
     3961 atgcgggtca agagcggcgg gtcgttgcac ttcgtcgcag tgccgctcgc caagggctga
     4021 ggttcgactg gtttacgaga tggagggtgt cgccggcatc agtcgccccc gccgacggct
     4081 ctttccgggg ggcgggttta ggaagcccgc tccctttagc tgccttccga tggaatacgc
     4141 ccccctctgt cgggaggtca aagggcggtg aagtccccca gcttcaccgc ccgctttttt
     4201 gctgaatcga taggttttac gctgccttgc atgaaggtgc cgaccgcgcc atggtgacag
     4261 aaagccgaac ccacttgacc gcaaccgacc cacaaatgcg cctcttgatc atcgaagacg
     4321 accgcgagtc cgccgactac ctggtgaagg cgtttcgcga agtcggctac gtcgccgatc
     4381 tcgccagcga tggcgaggaa ggcttgtcgc tggccgacag cggcgactat gacgtgctgg
     4441 tgatcgaccg gatgctgccg aaacgcgacg gcctgtcgct gatcggcacc ttgcgcgaga
     4501 agggcaatcg caccccggcg ctgattctct cggcgctcgg ccaggttgac gaccgcatca
     4561 agggtttgcg cgccggcggc gacgattact tgccgaagcc gtattcgttc gccgaactgc
     4621 tggcgcgggt cgaagtgctg tcgcgccgcc acggcggccc ggccgaggaa accacctatc
     4681 gggtcggcga cctcgagctc gatcgtcttt cgcatcgggt ggcgcgcggc aaggacgaat
     4741 tgacgctgca accgcgcgag ttccggttgc tggaatatct gatgaagcac gccgggcagg
     4801 tggtgacgcg aaccatgctg ctcgaaaacg tctgggatta tcatttcgat ccgcagacca
     4861 atgtcatcga cgtgcatatt tcgcggctgc gctccaaaat cgacaaggga tttgagcggc
     4921 cgctgcttca tacgattcgc ggcgcgggat acatgatccg tgacggcatc agataaaaag
     4981 acaaaatttc aatcacttgc ccattaggcg cggggttcac ctaccagttt attgccaatt
     5041 ttcgggcgcg acttgagccg ttaacgacaa gcaccggcca cggcgaagtg ctagccgaaa
     5101 cgtcatgatc ctgaccgtat gtctcgacga aagcgggaca cacgccgact cgcccatcag
     5161 tgtgatggca ggctat
//
LOCUS       DASWGM010000701         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_758779,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000701 DASWGM010000000
VERSION     DASWGM010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758779"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VGA15_19705"
     CDS             complement(<1..836)
                     /locus_tag="VGA15_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9589959.1"
                     /translation="MDIFILLTTMLVCFLIGMPIAYSLALAAIAGAWSIGIPLEAVML
                     KISDGVSKVAMLTIPFFVLAGAIMAEGGMARRLVAFADVLVGFTRVRGGLSIVNVLAT
                     TFLSGISGSAVADTSAIGSVMIPQMEKAGYPRVFATNLTITASVQALLVPPSHNAVLY
                     SLATGGTISISALFMAGVFPGLLLGFSLIVLCLVIAYRDKHPHGQTVAAKDALKITID
                     AAWGMVTLVIILGGILGGIFTAIEAGAVACIWAFFVTMFIYRDYRWRDLPVLLHRTLR
                     TVA"
     gene            complement(854..1369)
                     /locus_tag="VGA15_19710"
     CDS             complement(854..1369)
                     /locus_tag="VGA15_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX9589960.1"
                     /translation="MATLFRRAMDSLYLACVVVGCTALVLISAVIPWAVFTRYVLNSA
                     ASWPEPLAVLLTIVLTFIGAAAGYRLNLHMNVSYFADQLPAKLRTLLDLVVQLLMALI
                     AIFMIVWGGRLVEVTWYNTIADFPFLSVGVTYLPIPVGGVCLLLFIIERIFLGAPPDP
                     ITQDSHVVPID"
     gene            complement(1459..>2182)
                     /locus_tag="VGA15_19715"
     CDS             complement(1459..>2182)
                     /locus_tag="VGA15_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX9589961.1"
                     /translation="LRVSVGAMGPIVDDINVVNMPFLFKNTAHAQRMMDGPIGQELLD
                     KVTASPNAGLVALCWMDSGARSLYNTKKPIKAIEDLKGMKFRVIGNPIFVDMMNALGG
                     NGVAMGYDQVFSALQTGVIDGAENNPPSYVFSNHYTAAKYYSLTEHLIIPEMLMFSKK
                     AWTALSSEDQGLLKKFAREAQMEERELWKTYEQQAMEKAKAAGCEITEIADKAPFQNA
                     VKPVWDKYGPKYQDMIKRIQAV"
BASE COUNT          470 a          653 c          649 g          410 t
ORIGIN      
        1 gcgaccgtgc gcagcgttcg gtgcagcagg acgggcaggt cgcgccagcg atagtcccgg
       61 tagatgaaca tggtcacgaa aaacgcccag atgcaggcga ccgcgccggc ttcgatcgca
      121 gtgaagattc cgcccagaat accgccgagg atgatgacca gcgtcaccat gccccaggcc
      181 gcatcgatcg tgatcttgag cgcgtccttc gctgccacgg tctggccgtg ggggtgcttg
      241 tcgcggtaag cgatgacgag acagagaacg atcagcgaga atccgagcag cagcccgggg
      301 aagacaccgg ccatgaacaa ggcgctgatc gaaatcgtgc cgccggtggc cagcgaatac
      361 agcaccgcat tatgactcgg cgggaccagc agcgcctgaa ccgaggcggt gatggtgagg
      421 ttggtcgcga acaccctggg atagccggcc ttttccattt gcgggatcat caccgagccg
      481 atcgcggagg tatccgcgac cgcggagcct gaaatgccgc tcaggaatgt cgtggccagc
      541 acgttgacga tggaaaggcc gccgcgcacg cgggtaaatc cgaccagaac gtccgcgaaa
      601 gcaaccagcc ggcgcgccat gccgccttca gccatgatcg cgcccgccag cacgaaaaac
      661 ggaatcgtca gcatcgccac cttgctgacg ccatcggaga tcttcagcat caccgcttcc
      721 agcgggattc cgatcgacca ggcgcccgcg atcgccgcaa gcgccagcga gtaggcgatc
      781 ggcatgccga tcagaaagca gaccagcatg gtcgtaagca gaatgaagat atccatggat
      841 tgcctatctc gggtcagtcg atcggtacga cgtgcgagtc ttgagttatt ggatccggcg
      901 gcgccccaag aaaaatgcgc tcgataatga agaggagcag gcaaaccccg ccgacgggaa
      961 tcggcaggta ggtcacgccc accgacagga acgggaaatc cgcgatggta ttgtaccagg
     1021 tcacctcgac gagccggccg ccccacacga tcatgaatat cgcgatcagc gccatcagca
     1081 gctggacgac gagatcgagc agggtgcgga gcttcgccgg caactggtct gcgaagtagg
     1141 agacattcat gtgcaggttg agccgatatc ccgcggcagc gccaatgaag gtcagcacga
     1201 tggtcaggag aaccgcgagc ggctcgggcc aggacgccgc gctgttgagc acgtagcggg
     1261 taaataccgc ccatgggata actgccgaaa tcaacaccag cgcggtgcag ccgacgacga
     1321 cgcaagcaag atagagcgag tccatcgctc ggcgaaacag tgtggccatt tctcgatttt
     1381 cctgattgcg ccgaacgccg ccggacgctt gcggcgcgtt gatctgcgcg ccgcaagtgc
     1441 aggcggcaca ccaaatttct agacggcctg gattctcttg atcatgtcct gatatttcgg
     1501 gccgtatttg tcccacaccg gcttgaccgc gttctggaac ggcgccttat cggcaatctc
     1561 ggtgatttcg cagccggcgg ctttggcttt ttccatcgcc tgctgctcat atgttttcca
     1621 gagttcgcgc tcttccatct gcgcctcgcg ggcaaatttt ttcaagaggc cctggtcctc
     1681 gctcgaaagt gcggtccatg ccttcttcga gaacatcagc atttcgggaa tgatcaggtg
     1741 ctcggtgagg gagtaatatt ttgccgccgt gtagtgattg ctgaagacat agctcggcgg
     1801 attgttctca gcgccgtcaa tcacgccggt ctgtagcgcg ctgaacacct gatcgtagcc
     1861 catcgcgacg ccattgcccc ccagggcgtt catcatgtca acgaagatgg ggttgccaat
     1921 gacgcgaaac ttcatgccct tgaggtcttc gatcgccttt atcggtttct tcgtgttgta
     1981 gaggctgcgg gcaccggaat ccatccagca caaagcgacc agtccggcat tgggactcgc
     2041 tgtgacctta tccagcaact cctgcccgat cggtccatcc atcatccttt gcgcgtgcgc
     2101 cgtatttttg aacaggaacg gcatgttgac gacgttgata tcgtcgacaa taggtcccat
     2161 cgcccctacg ctcacacgca ac
//
LOCUS       DASWGM010000702         5309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_195163,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000702 DASWGM010000000
VERSION     DASWGM010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195163"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1165)
                     /locus_tag="VGA15_19720"
     CDS             complement(347..1165)
                     /locus_tag="VGA15_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9589962.1"
                     /translation="MQFVEANGAKIPAIGLGTWELRGRTCARLVEQALKLGYRHIDTA
                     QVYENEREVGEGLRASRVRRDELFVTTKVWTTHFAPNDLERSTKESLTKLRLSEVDLL
                     LLHWPNPHVPLAETLGALAHVRKLGMARHIGVSNFTVALIDEAVAACSEPLVCDQVEY
                     HPYLDQTRVRDACARHGMAVVAYSPVAKGRIKNDETLVQIGQTHGKTAAQVCLRWLVQ
                     QNVSAIPRTSRIERLSENIDIFDFALSPDEMRLISQMGSTKGRLTDFGFAPKWD"
     gene            complement(1374..1712)
                     /locus_tag="VGA15_19725"
     CDS             complement(1374..1712)
                     /locus_tag="VGA15_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9589963.1"
                     /translation="MQRLRLKADGRIVELRDGQEFPLAPVMTSAALATIPVPPAETET
                     FPAVRDLRRRARLTQLQFASRLGVPVETIRNWEQGKRMPRGPARALLAVIAHAPETVF
                     AALATDSSPV"
     gene            complement(1791..3212)
                     /gene="mgtE"
                     /locus_tag="VGA15_19730"
     CDS             complement(1791..3212)
                     /gene="mgtE"
                     /locus_tag="VGA15_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX9589964.1"
                     /translation="MADDVDVAHSADAPVLERLPMRDENGEIRREFVEDISRAIRVAD
                     AASLRAIVAELHEADLGDLIAALEPEDRVSLVELTGADFDFSALNEVDDSVREEILEE
                     LEPETVAEGVRELESDDAVELLESLDEEDQEEILEKLPPLERDALERSLLYPENSAGR
                     RMQTEFIAVPPDWTVGQAIDYMRDTPDLPDRFYEIYAVDKARHWQGAVSLDALLRSRR
                     PVPLVDLIDEDRRRVSVLDDQEEVARLFGKYNLVAAPVVDTTDRLVGVITIDDVVDVI
                     EEEADEDLKALGGVTSHEELSDSVWTIAKGRFNWLLVNLATAFLASSVLGFFEGQLEK
                     MVALAVLAPIVASQGGNAATQTMTVAVRALATRELGSNNAFRVVMREGLVGLVNGLAF
                     AVITGIAAVAWFKIPGLGVVIGLAIICNLVAGALGGILIPMVLERVRADPAVASGTFV
                     TTITDVVGFFSFLGIATLWFGLK"
     gene            3342..4115
                     /locus_tag="VGA15_19735"
     CDS             3342..4115
                     /locus_tag="VGA15_19735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9589965.1"
                     /translation="MTGAALVALIAWTAAAQAADCPRQGTLGTLRILSVDAATTPRVG
                     LKEYPQTLPLEDHEVVLTFDDGPWLPTTPRVLAALAQECVRATFFLIGKPASEHPDLV
                     RRIAAEGHTIGHHTWLHRSLMQIKPSETTEEIDHGISAVEMALRGVATSTPSTPFFRF
                     PGFETTPATLDLLQSRGIVVFGTDLWASDWDPMTPKHELKLIIDRLNIARKGIILFHD
                     PKARTAAMLPDFLRYLKDNHYRVVHLVPTGPGVDFDGAL"
     gene            complement(4174..4446)
                     /locus_tag="VGA15_19740"
     CDS             complement(4174..4446)
                     /locus_tag="VGA15_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589966.1"
                     /translation="MKAHAANLLDVSVSGVHLLVKGSIAGLDLKEFFLLLSSTGLAYR
                     RCELAKSKGPLTGIIELRTEGGHLEMGIDEDAALALRTDLERFLTQ"
     gene            4435..4632
                     /locus_tag="VGA15_19745"
     CDS             4435..4632
                     /locus_tag="VGA15_19745"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9589967.1"
                     /translation="MSLHRYLRLRRPWAVRRLLLAGEPLQIKACALSSGFWHMGEFAA
                     LYAAQFGEAPSMTQARSQRRP"
     gene            4794..>5309
                     /locus_tag="VGA15_19750"
     CDS             4794..>5309
                     /locus_tag="VGA15_19750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX9589968.1"
                     /translation="MVGKVLVGVRTRQWAALSLGLLTCITASIASAADCPGHPDALGT
                     SRTLVVDPRVHPRIGTMQYAETLPLRDHEVVLTFDDGPLPHNSNQILEILASQCVKAT
                     FFTVGSMAQNSPEGVRKLREAGHTIATHTQTHPIGFDKFSPERARQEIDEGIASVTAA
                     LGEGGGLAPFFR"
BASE COUNT         1028 a         1673 c         1619 g          989 t
ORIGIN      
        1 tgccgtcggc gtcggcgatg cggcctgtcg cggtgccggt gaagccgcag gcgcggattt
       61 cgatgaaaga tcgaaggcgt cagagccttg agatttcggc tctggttcgg gctttctcgg
      121 aacctgcacg gcttcttcgg gcgaaactat atcaaccgcg atcggctcgg cagttaccga
      181 agcgaacgga tgcacctcgg caaagaccag gaccaccgcc agaattgaca gatggccaag
      241 cgccgatgct gcgaagcccg agcgtatgaa ccgaaggggt tccaccgtat tggatcgttt
      301 ctatgccgcc tctcgccgac agcatagcgg gcaacggcgc cgcgtctcaa tcccatttcg
      361 gcgcgaaacc gaaatcggtg agccggcctt tggtgctgcc catctgggat atcagccgca
      421 tctcgtcggg cgacagcgcg aagtcgaaaa tgtcgatgtt ttccgacagc cgttcgatcc
      481 gcgacgtccg cggtatcgcg gagacattct gctgcaccag ccagcgcagg cagacctgcg
      541 cagccgtctt gccgtgggtc tgtccgatct gcaccagtgt ctcgtcgttc ttgatacgcc
      601 ctttcgcaac cggactatag gcgacaaccg ccatgccgtg acgtgcgcag gcatcccgca
      661 ccctggtctg gtcgagataa ggatggtatt ccacctgatc gcagaccaga ggctcggaac
      721 aggcggcaac cgcctcgtcg atcaaagcca cggtgaagtt cgagacgccg atgtgcctgg
      781 ccatgccgag cttcctgaca tgggccagcg cgcccaacgt ctcggccagc ggcacgtgcg
      841 gatttggcca atgcagcagc aacagatcga cttcggaaag ccgcagtttc gtcaggctct
      901 ccttggtgga tcgttccaga tcattcggtg cgaaatgggt ggtccagact ttggtcgtca
      961 cgaacaattc gtcgcgcctg accctcgaag cgcgcagacc ctcgccgacc tcgcgttcgt
     1021 tctcatagac ctgcgcggtg tcgatgtggc gatagccgag cttcagcgcc tgctcgacca
     1081 ggcgcgcgca ggttcgcccg cgcagttccc aggtgcccag cccgatcgcg gggatttttg
     1141 cgccattggc ctcaacgaat tgcatcgcgg attacccgat catactagag cctttccgtt
     1201 tcgatagaat cgaaacgggg ctccagattc ttgatttgac gcattttctt gacgcgaacc
     1261 ggtctccacc cccggatcaa gtccgagggc atgcttcgct tgaaaacgct ccaagatcat
     1321 cccagtatgt cccttgggcg tcgcgctgcc aacatcgcgg gctgcagttt cgctcagact
     1381 ggcgaagagt ccgttgccag agcagcgaaa accgtctcgg gggcatgggc gatcacggcg
     1441 agaagcgctc gcgccggtcc gcgcggcatg cgtttaccct gctcccagtt gcggatggtc
     1501 tcaacgggga cgccgagccg gcttgcgaat tgcagctggg tcaggcgggc tcggcggcga
     1561 aggtcacgca cggccgggaa cgtttccgtc tccgccggcg ggacgggaat agtcgcgagc
     1621 gccgcggaag tcatcaccgg tgcgagcgga aactcctgcc cgtcccgcag ttcgacgatc
     1681 cgtccatccg ctttcagccg caagcgttgc atgctcgact ccgcacggga atagtcggcg
     1741 atgcgcgtta aggcttgatt aacgatgcgc gacccgaggg cggcatcgtt ctatttcaga
     1801 ccgaaccata gcgtggcgat gccgagaaac gaaaagaagc cgaccacgtc ggtgatggtc
     1861 gtgacaaagg ttcccgacgc caccgccgga tcggcgcgca cccgctccag caccatcgga
     1921 atcagaatgc cgccaagcgc gcccgccacc agattgcata tgatcgcgag tccaatcacg
     1981 acgccaagcc ctggaatctt gaaccaggcc acggcggcga taccggtgat aacggcaaat
     2041 gcgaggccgt tgacgaggcc gaccaggccc tcgcgcatca ccacgcgaaa ggcgttattc
     2101 gatccgagct cacgggtagc caaagcgcgc accgcgaccg tcatggtctg ggtcgctgca
     2161 ttgccgccct ggctggcgac gatcggcgcc agcaccgcca acgccaccat cttttccagt
     2221 tgaccttcga aaaagcccag caccgaggac gccagaaagg cggtggcaag attgaccaac
     2281 agccagttga agcggccctt ggcgatggtc cagacactgt cggataattc ttcgtggctg
     2341 gtgacgccgc cgagcgcctt caggtcctcg tcggcctctt cctcgatcac gtccaccacg
     2401 tcgtcgatgg tgatgacgcc gaccagccga tccgtggtgt cgacgacggg ggctgcgacc
     2461 agattgtact tgccgaacag ccgcgccacc tcttcctggt cgtccagcac ggagacgcgg
     2521 cggcggtctt catcgatcag gtcgacaagc gggaccggcc tgcgcgaacg caacagcgcg
     2581 tccagcgata ccgcgccctg ccagtgccgg gccttatcga ccgcgtagat ttcgtaaaac
     2641 cggtccggca aatccggcgt gtcgcgcatg tagtcgatcg cctgcccgac ggtccagtcc
     2701 ggcggcacgg cgatgaactc ggtctgcatc cggcggccgg ctgaattttc cgggtaaagc
     2761 aggctgcgct cgagcgcgtc gcgctccaat ggcggcagtt tttcgaggat ctcttcctgg
     2821 tcttcttcgt cgaggctctc aagcaattcg acggcgtcgt cggattccag ttcgcgtaca
     2881 ccctccgcga ccgtctcggg ttcgagttcc tcgaggattt cctcgcggac ggagtcgtcg
     2941 acttcattga gcgccgagaa gtcgaaatcc gcgccggtca gttctacgag gctgacgcgg
     3001 tcctcgggtt cgagcgccgc gatcagatcg ccgagatcgg cctcgtggag ttccgcgacg
     3061 atggcgcgta acgaggccgc gtcggcaaca cgtatcgcgc gcgagatgtc ttcgacgaac
     3121 tcgcggcgaa tttcgccatt ttcgtcgcgc atcgggagac gctcaagcac cggcgcgtcg
     3181 gcggaatggg caacgtccac atcatcggcc atgccgcgcc tcgccggttt gacagaattg
     3241 ctgaactcgt ctgagttgtc gcatcacgca ttacccaatg ggcaatacca agcgcaatga
     3301 caaatatcga ccggctaaaa cgggctcgga agttcgtgaa cgtgacaggg gctgccctgg
     3361 ttgccttgat cgcgtggaca gcggcggcgc aggcggcgga ctgcccgcgc caaggcacgc
     3421 tcggcacctt gcgcattctt agtgtcgatg ccgcgaccac tccgcgggta ggactgaaag
     3481 aatatccgca gacgctgccg ctggaggacc acgaggtcgt gctgacgttc gatgacggcc
     3541 cgtggctccc gaccacgccg cgggtgctgg cagcgctggc ccaagagtgt gtgcgcgcga
     3601 ccttcttcct gatcgggaag ccggcgtccg aacacccgga cctggtgcgg aggatcgcgg
     3661 ccgagggcca caccatcggc catcatacct ggttgcatcg cagcctgatg caaatcaagc
     3721 cgagtgaaac aacagaggaa atcgaccacg gcatatcggc ggttgagatg gcgctgcgcg
     3781 gcgtcgcgac cagcacgcca tccacgccgt tctttcgctt tccgggcttt gagacgaccc
     3841 cggcgacact tgacctgctg caatcgcgcg gcatcgtcgt gttcggaacc gacctttggg
     3901 ccagcgactg ggatccgatg acgccgaagc atgaattgaa gctgatcatc gacaggctca
     3961 acatcgcccg caagggcatc atcctgtttc acgaccccaa ggcgcgaacc gcggccatgc
     4021 tgccggattt cttgcgctat ctgaaggata atcactatcg ggtggtccat ctggtgccga
     4081 ccggacccgg cgtcgacttt gacggcgccc tctgagaccg tacgcggaag tcgatcggca
     4141 gcggcaaaag acagacctcc cccgaaacgt ggctcactgg gtgagaaaac gctcgaggtc
     4201 ggtacgcagc gcgagcgccg cgtcttcgtc gattcccatt tcaagatgcc cgccctcggt
     4261 tcgcaattcg ataattccgg tcaacggccc tttcgatttg gccaattcgc aacgccgata
     4321 ggcaagaccg gtcgacgaca acagaagaaa gaattccttt aagtccaacc cggcaatcga
     4381 ccctttgaca agaaggtgaa cgccggacac ggagacatcg agcagattcg cggcatgagc
     4441 cttcatcggt atcttcgatt gaggcggccg tgggcagtaa ggaggctgtt gctcgccggc
     4501 gagccattgc agatcaaggc atgcgcactt tccagcggct tttggcatat gggcgagttc
     4561 gcggccctgt atgccgccca attcggcgaa gcgccgtcaa tgacgcaggc tcgctcgcag
     4621 cggcggccat gatgaaacta tggtgaaaag tagcgcggca ggccgcagtt gccttggatt
     4681 gggcccaaaa gcatggtttg cctgacaatg ctctgaaatt acacgcaatt cacgccgcat
     4741 tcatggtgcc gcgtgtaatt gtcagcgcaa ctgggaatgc atgacggtgt tttatggtcg
     4801 gtaaagtgtt ggtaggcgtt cggacccggc aatgggccgc cttgtccctg gggctgctga
     4861 cctgcatcac cgcctcaatc gcgtcggccg ccgattgtcc cggccatccc gatgcgctcg
     4921 gcacctcgcg aacgctggtg gttgatccga gggtgcatcc ccggatcgga acgatgcaat
     4981 atgccgagac cctaccgctg cgggatcacg aagtggtgct gaccttcgat gacggcccgc
     5041 tgccgcacaa cagcaatcag atcctggaaa ttctggcctc gcaatgcgtc aaggcgacgt
     5101 tcttcaccgt cggcagcatg gcccaaaatt ctcccgaggg cgtccgcaag ctccgcgagg
     5161 ccgggcatac catcgccacc cacacccaga ctcatccgat cggcttcgac aagttctcgc
     5221 ccgagcgcgc caggcaggag atcgacgagg ggatcgcgtc ggtgacggca gcactcggtg
     5281 agggcggcgg gcttgctccg ttcttccgg
//
LOCUS       DASWGM010000703         4628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2588210,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000703 DASWGM010000000
VERSION     DASWGM010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588210"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VGA15_19755"
     CDS             <1..142
                     /locus_tag="VGA15_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX9589969.1"
                     /translation="DLTVKIPGEIDVQRVRRILEELRRRLADPQRPQIELDYIERLLK
                     DY"
     gene            complement(351..728)
                     /locus_tag="VGA15_19760"
     CDS             complement(351..728)
                     /locus_tag="VGA15_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9589970.1"
                     /translation="MPRVLIADDEDSMRTLVARAVAMDGHDTVTAEDGAEALEILIRE
                     QGAFDLLLTDIQMPVMDGIALALTAARDFPDLTILLMTGFAAQRERAHGLNAIVHDVI
                     TKPFSVADIRTAVADALAAGKKG"
     gene            complement(769..1635)
                     /locus_tag="VGA15_19765"
     CDS             complement(769..1635)
                     /locus_tag="VGA15_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEX9589971.1"
                     /translation="MHIICPHCTTSYAIDLATLGASGRTVRCSRCKEVWLARPQDATE
                     IAASVPAMAEAGQTSAADPAAEWDALAREDDSHETPIVDSPSISADWPADGQGAPQDS
                     ENDWPSIARQDGSDPETARPQRLSWLRRLVKPPLRASGKPLVSLPTVCAVMGALVLAL
                     MIWRVDVVRLLPQTAAFYKLAGFDVNLRGLAFKDIKIATETVEGKPVLVIEGAIVGEA
                     RKPVELPRLRFSVRDAQGAEIYAWNAVLDQLVLKPGETAWFKLRLASPPPEGRNIDVR
                     FFGKRDVAAGDT"
     gene            1810..2469
                     /gene="ftsE"
                     /locus_tag="VGA15_19770"
     CDS             1810..2469
                     /gene="ftsE"
                     /locus_tag="VGA15_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX9589972.1"
                     /translation="MVRFENVGLRYGLGPEILRDLSFQIPAHSFQFLTGPSGAGKTSL
                     LRLLFLSLRPTRGLVNLFGHDVSMLGKDDVADLRKRIGIVLQDFRLLDHMTTYENVAL
                     PFRVIGREESSYRKEVIDLLKWVGLGERMDALPPVLSGGEKQRAAIARAVISRPLLLL
                     ADEPTGNVDPTLGRRLLRLFIELNKSGTAVIIATHDITLMDQYEARRMVLHHGRLHIY
                     E"
     gene            2462..3433
                     /locus_tag="VGA15_19775"
     CDS             2462..3433
                     /locus_tag="VGA15_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9589973.1"
                     /translation="MNRMGDERGLLVDLGQERPRVAASARNLSPIVPRGSIAGRALVA
                     VVAIMTFLASMTTGAVLLISASAAEWQSEVASEITIQVRPLPGRDIEHDTLAVADAIR
                     AQPGIVDVRPFTRDESAKLLEPWLGTGLTLDDLPVPRVVVARVQPGTTLDLAALRSRV
                     TKAAQSASVDDHRAWIERMRSMTGATVLAGIGILALVIIATIISVSFATRGAMAANRP
                     IVEVLHFVGAGDRYIANRFLRHFLRLGLQGGVIGGGVAMFTFGFSESIAGWFSGTPIG
                     DQFGALLGTFALRPSGYLALAAQAALIAAITAWASRRTLFATLDDID"
     gene            3624..4343
                     /locus_tag="VGA15_19780"
     CDS             3624..4343
                     /locus_tag="VGA15_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX9589974.1"
                     /translation="MSLQPDDRSPNAPSSARRGWLRATIVAMLAIVFVTAAIGFVGFL
                     SKLRGAEMKPAGNADGIVVLTGGASRVSDAMELLAGGYGKRLLISGVHPTNAASDISR
                     SLPDNQSLLSCCVDLDRSAVNTRSNAAETRRWARDRGFKSLIVVTSNYHMPRAIVELS
                     HAMPGVALIPFAVVGDKWRDEPWWTNGATLRLLLSEYVKYVAAEVRVRLADAGLELTP
                     ELADQPGGSLAPPRPATAQAN"
     gene            4355..>4628
                     /locus_tag="VGA15_19785"
     CDS             4355..>4628
                     /locus_tag="VGA15_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX9589975.1"
                     /translation="MVSIFLRSLVYNVLFYALLVFWILIAIPTYLMPPRAFMAIAKAW
                     SRSSVWLMRVICNTKVEYRGLEKIPKGPLIVASKHQSMWETFALMQF"
BASE COUNT          762 a         1428 c         1547 g          891 t
ORIGIN      
        1 cgatctgacg gtgaagattc ccggcgagat cgatgtccag cgggtgcgcc ggattctgga
       61 agaactgcgc cgccgcctcg ccgatccgca gcggccccag atcgagctcg attacatcga
      121 gcggctgctg aaggattatt aaggcttcgt attcatacgg accgtcgtca cccgggcttg
      181 tcatacgcgg gcagaagcgc gaagcgcgtc ttcgcgctag atgacccgcg tatccatcat
      241 cctcacggga gtctttcgaa gacgatggat tgccgggagc gcagacaagt ttacgtcgtc
      301 tgcgcaaggc agactactat gcccggcaat gacggatgga agctcctccg ctagcctttc
      361 ttgcccgcgg ccagtgcgtc ggcgaccgcg gtgcggatat cggccaccga gaacggcttg
      421 gtgatgacgt cgtggacgat ggcgttgagg ccgtgggcgc gctcgcgctg ggcggcgaac
      481 cccgtcatca acagaatggt cagatcgggg aaatcgcgcg ccgccgtcag cgccagcgcg
      541 atcccgtcca tcaccggcat ctggatatcg gtgagcagga gatcgaacgc gccctgttcg
      601 cggatcagga tctccagcgc ttcggcgccg tcttccgcgg tgacggtgtc gtggccgtcc
      661 atggcaacgg cacgggccac cagcgtgcgc atggagtctt catcgtcggc gatcagcacg
      721 cgcggcatgg atgcccccgt ccttggcgag cgggccgggc ggccccgttc atgtgtcgcc
      781 ggcggcgaca tcccgcttgc cgaagaagcg tacatcaata ttgcgccctt cgggcggcgg
      841 cgaagccagt cgcaacttga accaggctgt ttcgcccggc ttcagcacca gttggtccag
      901 caccgcgttc caggcataga tctcggcgcc ctgggcgtcg cgaacggaaa agcgcagccg
      961 cggcaactcg accggcttgc gggcttcgcc gacgatggcg ccttcgatta ccagcaccgg
     1021 tttaccttcc accgtctcgg tggcgatctt gatgtccttg aatgcaagcc cgcgcaggtt
     1081 cacgtcgaag ccggccagtt tgtagaatgc cgcggtttgt ggcagcagcc gcaccacgtc
     1141 gacgcgccag atcatcagcg ccagcaccag cgcgcccatg acggcgcaga ccgtaggcag
     1201 gctgaccagc ggcttgcccg aagcccggag cggcggtttc accagcctgc ggagccacga
     1261 caggcgttgc gggcgggcgg tctcggggtc ggacccgtct tgccgggcta tcgacggcca
     1321 gtcgttttcg ctatcctggg gggcgccttg gccgtcggcc ggccagtcgg cggatatcga
     1381 ggggctgtcg acgatcgggg tttcgtggct gtcgtcttcg cgcgcaagcg cgtcccattc
     1441 cgcggcgggg tctgcggctg aggtctgtcc ggcctccgcc atcgcaggca ccgacgctgc
     1501 tatctcggtc gcatcctgtg gccgcgccag ccagacctcc ttgcagcggg agcagcggac
     1561 ggttcggccg gatgcgccca atgtcgccaa atcaatggca taggacgttg tacaatgggg
     1621 gcaaatgatg tgcatggagc caatcgccac aatgacgctg ccgaaatgct acaaggcgac
     1681 cgttaacgaa acggaaacca taaccgaagc acaaccattt tgtcgggttc gcagccgcgg
     1741 cgtgccaatg agcttgcggg cctccggttt cgggtttccg gccaggccct acatcgaacg
     1801 gagctgagct tggttcggtt cgaaaatgtc ggattgcgct acgggcttgg cccggagatt
     1861 ctgcgcgacc tcagtttcca gatccctgcc cattccttcc agttcctcac cggcccctcc
     1921 ggcgccggca agacctcgct gctgaggctg ctgtttttgt cgctgcggcc gacccgcggc
     1981 ctggtcaatc tgttcggcca tgacgtctcg atgctcggca aggatgacgt ggccgatctg
     2041 cgcaagcgga tcggcatcgt gctgcaggac tttcgcttgc tcgaccacat gactacctat
     2101 gaaaacgtgg cgctgccctt ccgggtgata gggcgcgagg aatcgagcta tcgcaaggag
     2161 gttatcgatc tcttgaaatg ggtcggtctc ggcgagcgga tggatgcgct cccgccggtg
     2221 ctgtccggcg gcgagaagca gcgtgcggcg atcgcacgcg cggtgatttc gcgcccgctg
     2281 ctgttgctgg cggatgagcc gaccggtaac gtcgatccga ccctggggcg acgattgctg
     2341 cgcttgttca tcgaactgaa caagtccggg acggcggtga ttatcgcgac ccacgacatt
     2401 acgctgatgg atcagtatga ggcgcggcgg atggtgctgc atcacggacg gttgcatatc
     2461 tatgaataga atgggtgacg agcgcggcct gttggtggat ttggggcaag agcggccgcg
     2521 ggtggcggcg agcgcgcgca atctgtcgcc gatcgtgccg cggggctcga tcgccggccg
     2581 cgcgctggtc gcggtcgtcg ccatcatgac gttcctcgca tcgatgacca cgggcgcggt
     2641 gctgctgatc agcgcgtcgg cggcggagtg gcagtcggag gtcgcgagcg aaatcaccat
     2701 ccaggtgcgc ccgttgcccg ggcgcgatat cgaacacgat actttagcgg tagctgacgc
     2761 gatcagggcg cagccgggca tcgtcgatgt caggcctttc accagggacg agtccgccaa
     2821 attgctggag ccatggctgg gcaccggact gacgctcgac gatctgccgg tgccgcgcgt
     2881 cgttgtggcg cgggtgcagc ccggaacgac gctggatctg gcggcgctgc gcagccgggt
     2941 gacgaaggcg gcgcagtcgg ccagcgtcga cgatcaccgc gcctggatcg agcggatgcg
     3001 atcgatgacc ggcgcaaccg tgcttgccgg catcggcatc ctggccctgg tgattattgc
     3061 gaccatcatt tcggtatcgt tcgcgacccg tggcgcgatg gcggccaacc gtccgatcgt
     3121 cgaagtcctg cacttcgttg gagccggaga ccgctacatc gccaaccggt tcctgcggca
     3181 ttttctccgg ctggggctgc agggcggcgt gatcggcggc ggcgtcgcga tgttcacttt
     3241 cgggttttcc gaatccatcg ccggctggtt ctcgggcacc ccgatcgggg accaattcgg
     3301 ggccttgctg gggacgttcg cgttgcgccc gtccggctat ctggcgctgg cggcgcaggc
     3361 ggcgctgatc gcggcgatca ccgcctgggc gtcgcggcga acgctgtttg ccacgcttga
     3421 cgacatcgac tgatgcggct ggcccgaatc ccgcgccggc ccgactctca ttcgtctgaa
     3481 aacgttctga agcgttttgg agcgaaagcc tgccccggtt tgcggcaaga agacgcgtca
     3541 aatcagcaat ccagagcccc gtttcgattc tatcgaaacg gaaaagcctc tagactgagt
     3601 gcgcggaggg tcgccagcat cacatgagtc tccagcccga cgatagatcg ccaaacgcgc
     3661 cgagttctgc gcggcggggc tggctgcgcg caacgatcgt agcgatgttg gcgatcgtgt
     3721 tcgtcaccgc ggcgatcggc tttgtcggct ttctctcgaa gttgcgcggt gctgaaatga
     3781 agcccgcagg caatgccgac ggcatcgtgg tgctgaccgg cggcgcgtcg cgggtttctg
     3841 acgcgatgga gttgctcgcc ggcggctatg gcaagcggct gttgatctcg ggcgtccatc
     3901 cgaccaatgc cgcgagcgac atctcgcgtt cgctgccgga taatcaatcg ttgctgagct
     3961 gctgcgtcga tctcgaccgc tccgccgtca acacccgcag caacgcggcg gaaacccggc
     4021 gctgggcgcg cgaccgcggc ttcaagtcgc tgatcgtggt gacatcgaat taccacatgc
     4081 cgcgggcaat cgtcgaattg tcgcatgcga tgcccggcgt cgcgctgatt ccgttcgcgg
     4141 tggtcggcga caaatggcgg gatgagccgt ggtggacgaa tggcgcgacg ttgcggctgc
     4201 tgctatcgga gtacgtgaaa tacgtcgccg ccgaggtgcg ggtgcgtctg gctgacgccg
     4261 gcctcgaact gacgcccgag ctcgcggatc agccgggcgg atctctcgcg ccgcccagac
     4321 cggcgacggc tcaagccaat tgatcgggtt ttcgatggtt tcgattttct tgcgttcgct
     4381 ggtctacaac gtgctgttct atgcgctgct ggtgttctgg attctgattg caatccccac
     4441 ctatctgatg ccgccgcggg ccttcatggc catcgcgaag gcctggtcgc gcagcagcgt
     4501 ctggctgatg cgcgtgatct gcaacaccaa ggtggaatat cgcgggctgg agaaaattcc
     4561 gaaagggccc ctgatcgtcg cgtcgaagca tcagtcgatg tgggaaacct tcgcgctgat
     4621 gcagttct
//
LOCUS       DASWGM010000704         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_616186,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000704 DASWGM010000000
VERSION     DASWGM010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616186"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VGA15_19790"
     CDS             complement(<1..878)
                     /locus_tag="VGA15_19790"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX9589976.1"
                     /translation="MPFTLPPDANGILPDRMIAAMADAGLILPAYPFVESQIQPASLD
                     LRLGDVAYRVRASFLPGPGATVAERIDELKLHEINLSDGAVLETNCVYIVPLLESLAL
                     PPQIVAAANPKSSTGRLDVFTRVIADGTRRFDMIGAGYHGPLYAEISPKTFPVLLREG
                     SRLSQVRFRTGDAILNADELDALHDLERLVDCDDADLLNGVALSVDLSGESANGFVGY
                     RAKRHTGVVDIDRRGGYAVDEFWEAISARPDGSLILDPGEFYILASKEAVQIPPDYAA
                     EMMPFDPLVGEFRVHYA"
     regulatory      990..1069
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1137..2342
                     /locus_tag="VGA15_19795"
     CDS             1137..2342
                     /locus_tag="VGA15_19795"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX9589977.1"
                     /translation="MSEVTLPVSSTAHYRPETRLVHSGTLRSQFGETSEALFLTQGFV
                     YDSAEQCEARFKGEDPGFLYSRFSNPNVSMFERRMIELEGAEAARATATGMAAVTTAV
                     LAPLKAGDHVVAAKAMFGSCRYVVEDLLPRYGIGSTLVDGLDLDQWRRAMRPNTRTCF
                     LESPTNPTLDVLDIGAIAEIAHQGGARLIVDNVFATPIWQSPLELGADVVVYSATKHI
                     DGQGRCLGGIILSSEAFIAEHIHTFMRQTGPSMSPFNAWVLLKGLETLAVRVRAQTET
                     AGAVADALARHPKISRLIYPGRSDHPQAALVKKQMRAGSTLVGFEVKGGKAAAFRCLN
                     ALKLSRISNNLGDAKSLVTHPSTTTHQRLTPEARAELGISEGFIRFSAGLEHRDDLIE
                     DLQAALETA"
     gene            complement(2404..>2634)
                     /locus_tag="VGA15_19800"
     CDS             complement(2404..>2634)
                     /locus_tag="VGA15_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX9589978.1"
                     /translation="ARLRHVGFFPRFEVMREWHEQQAIPIDNFVIADGLHMTDWGYAC
                     FAQLLGDDIIKSVGQIKLGVNVPSNVQTYRPM"
BASE COUNT          490 a          833 c          831 g          480 t
ORIGIN      
        1 gcataatgca cgcggaattc gccgaccagg gggtcgaacg gcatcatctc cgcggcgtag
       61 tccggcggaa tctgcacggc ttccttcgag gccagaatgt agaactcgcc gggatcgaga
      121 atcaggctgc cgtcgggccg cgcggatatc gcctcccaga attcatcgac cgcatagccg
      181 ccgcggcggt cgatatcgac cacgccggtg tgacgcttgg cgcggtaacc gacgaagcca
      241 ttggcgcttt cgccgctcag atcgacgctg agcgccacgc cgttgagaag atcggcgtcg
      301 tcgcaatcga ccagccgctc cagatcatgc agcgcgtcga gctcatcggc attcaggatg
      361 gcatccccgg tgcgaaaccg cacctggctg agccgcgatc cctcgcgcag caacaccgga
      421 aacgttttcg gactgatctc ggcatagagc gggccgtggt agccggcgcc gatcatgtcg
      481 aaccgccgcg taccgtccgc gatcacccgg gtgaacacgt cgagccgtcc ggtcgagctt
      541 ttcggattgg cggcggcgac gatttgcggc ggcagcgcca gactctccag cagcggcacg
      601 atgtagacgc agttggtctc cagcaccgcg ccatcgctca gattgatttc atgcagcttc
      661 aattcgtcga tgcgctcggc caccgtcgcg ccgggccccg gcaggaaact cgcgcgcacc
      721 cggtaggcga cgtcgccgag ccgcagatcg aggctcgcgg gctggatctg gctctcgaca
      781 aagggatagg cgggcagaat aaggccggca tccgccatcg ccgcgatcat gcgatcgggc
      841 aggatgccat tggcgtcggg tggaagtgtg aacggcacgt ctgattgtcc ttgccaacgc
      901 gtccctgcca tccagacgct caaaatacgg acttttatgc gttatccgat ggacggcttg
      961 acggaaaggg cgtgagcgat tatgagcatg acttatcccg tggtgatttg agccggccgg
     1021 cttgcagcca cgttaaataa gtcgctaaac aggccgggga catgtgtgat cccggcctgt
     1081 ggaatttttc caggccggtt ttttgtgacc attcctgtgg tcacgatgga ggtcacatgt
     1141 ctgaggttac gttaccggtt tcgtcaaccg cccactaccg tcccgaaacc cggctggtgc
     1201 attccggcac gcttcgctcg caattcggcg aaacgtccga ggcgctgttc ctgacgcagg
     1261 gctttgtcta cgacagcgcc gaacaatgcg aggcgcggtt caagggcgag gatcccggct
     1321 ttctgtattc gcgcttttcc aatcccaacg tgtcgatgtt cgagcgccgc atgatcgagc
     1381 tcgaaggcgc ggaagcggcg cgcgccaccg ccaccggcat ggccgcggtg accacggccg
     1441 tgctggcgcc gctgaaagcc ggcgatcacg tggtggccgc gaaagccatg ttcggctcgt
     1501 gccgctacgt ggtggaagat ctgctgccgc gctacggcat tgggtccact ttggtcgacg
     1561 gcctcgatct cgaccaatgg agacgagcga tgcggccgaa cacaaggacc tgctttctgg
     1621 aaagcccgac caatccgacg ctcgacgtgc tcgacatcgg cgcgattgcc gaaatcgccc
     1681 accaaggcgg cgcacggctg atcgtcgaca acgtgttcgc gacgccgatc tggcagagcc
     1741 cgcttgaatt gggcgccgac gtcgtggtgt attccgccac caagcacatc gacggccagg
     1801 gccgctgtct cggcggaatc attctctcct cggaggcgtt cattgccgag cacatccaca
     1861 ccttcatgcg ccagaccggc ccgtcgatgt cgccgttcaa cgcctgggtg ctgctgaagg
     1921 ggctggagac gctggcggtc cgcgtccgcg cccagaccga aaccgccggc gccgttgccg
     1981 acgcgctggc gcggcatcca aaaatctcgc ggctgattta ccccgggcga tcagatcatc
     2041 cgcaggccgc cctggtgaag aagcagatgc gcgcgggatc gacgctggtc ggcttcgagg
     2101 tcaaaggcgg caaggcggcg gcgttccgtt gtctcaacgc gctgaagctg tcgcggattt
     2161 caaacaatct cggcgacgcc aagagcctgg tcacccatcc gtcgaccacc acccatcagc
     2221 ggctgacgcc ggaagcccgc gccgaactcg gcatcagcga gggttttatc cggttctcgg
     2281 cggggctcga gcaccgcgac gatctgatcg aggatttgca agccgcgctg gagacggcgt
     2341 gaggggagcg cgcgaatagc gaatggcaat cttccctatt cgctactccc tattcgctca
     2401 aaatcacatc ggccggtagg tctgcacatt gcttggcacg ttgacgccga gcttgatctg
     2461 gccgaccgac ttgatgatgt cgtcgccgag caactgggcg aagcaggcat agccccaatc
     2521 ggtcatgtgc aggccgtcgg caatgacaaa gttgtcgatc gggatcgcct gctgctcgtg
     2581 ccattcgcgc atcacctcga agcgcgggaa gaaaccgacg tggcgcagcc gggc
//
LOCUS       DASWGM010000705         5885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2307408,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000705 DASWGM010000000
VERSION     DASWGM010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307408"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VGA15_19805"
     CDS             <1..432
                     /locus_tag="VGA15_19805"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX9589979.1"
                     /translation="TIIIDTPNTYLYYVLGGGQAVRYGIGVGRDGFTWSGVQSITRKA
                     EWPDWTPPPEMIARQPYLPRHMAGGPGNPLGARAMYLGGTVYRIHGTNAPETIGTQVS
                     SGRIRLTNEDVSDLYARVNVGTKVVVRPMDRRADNTDGKRS"
     gene            599..2155
                     /locus_tag="VGA15_19810"
     CDS             599..2155
                     /locus_tag="VGA15_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HEX9589980.1"
                     /translation="MRLKTIPLGVAVVAVSFFVSLKAMDWLSPRATVPAPVLVELPPL
                     PPAPRSSSIVAPIVIALSAIRDAADRGAPRTFAGKADNPVSQILQNADIGWTASRGPI
                     SATGAQDVLSLATPLTGTLNATGSLSAKATGAVGDALGSLLGGDVAKRIGSVNIKSLN
                     AHAEIKGNVTITARPKLAAAWRIEPNLTAQVTLSDTNLSVSGARVNVPAQVKPLIDKT
                     VADQLDAVQARIRNDPAFENNARLQWAKACRSIPLQSPGTSSSLPALWLELRPTRAIA
                     AQPRIDASAVTLTLGIEAETRITPAQTKPDCPFPATIDIVPPGPSRISIGVPIDLPFT
                     DINKIVEAQFAGRSFPEDGSGSVDVTVKRASVAASGDRLLISLLVKAKEKKSFVGLGG
                     EANIHIWGKPVLDQEQQTLRLTDVELAVESEAAFGLLGAAARAAVPHLQKVLADKATI
                     DLKPFAANAQNKIAAVISDFQKNEDGVRIAAEITSLRLADIAFDSKTLRVIAEAAGAI
                     NVSITALPGL"
     gene            2280..3407
                     /locus_tag="VGA15_19815"
     CDS             2280..3407
                     /locus_tag="VGA15_19815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9589981.1"
                     /translation="MQRLSLLLAAVVLCAVLLVAGCGDNKQPNPAVSAPPPAVTVVKV
                     TAAEIKPAKTFTGRIEAKDKVDLRVRVDGFLEKRLFTEGADVKEGELLFVIEKGLYQA
                     AVDQAKAGLETAQATLKLADIDVDRQTQLMQRNVTAQATLDQSTARQGEARGNMLAQQ
                     AALEKAELQLSYTDIKSPISGRIGRSSVSVGNFVSPSNSALATIVSQDPIYASFPVTQ
                     REMLDVRKQQAASKGSEYVIYIQLADGSRYPGAGKIDFLDNTVNQGTDTVQVRAVFAN
                     PDRVLVDGQLVTVVAEEGKGESALLVPQQALQVDQTGPYVLVVDKDNKIQIRRVETDV
                     ARGVNIVIRKGLAANELIVAEGIQRVRPGQVVAPTEAKPGA"
     gene            3411..>5885
                     /locus_tag="VGA15_19820"
     CDS             3411..>5885
                     /locus_tag="VGA15_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017271107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9589982.1"
                     /translation="MLSSVFIDRPRLAVVISIVITVAGLVALFQIPIAQFPNIVPPQI
                     AVTASYAGASAEVVEATVAQPIEARVIGVDNMLYMKSTSGNDGSYTLNITFAVGTDPD
                     LNTVKVQNRVGLAEAQLPAEVRAQGISVTKKSSALLQVIALTSPDGRYDQLFLSNYAT
                     INVIDQLKRVPGVGDVSLFSPSDYSMKVWLDTDRLTIFGLVPSDVAAALKSQNIQAAV
                     GRIGAQPALPDQQFQLNIQSKGRLSSVEEFGAVVVRANPDGSFVRVRDVARVELAGRL
                     SESLGRLDGQPAAVIGIYQSPGGNALDSAQRIGKILQELKTSFPEGVDYKITYDTTIF
                     VKESIKGVLHTLVEAFILVVIVVFLFLGSVRATLIPLLAVPVALIGTFAVMLALGYSA
                     NTVSLLALVLAIGIVVDDAIVVVEAVEAQLEKDHSLSPAEASRRAMAEITAPIIAITL
                     VLLSVFVPVAFIPGISGELFRQFAVAVSVSMVISAINALTLSPALCAVFLSASHGPKR
                     GLIGYVLRGIDKARDGYSLVVHKTVRFAVYSLLVLVAVIAGAGWLFKVTPTGFLPSED
                     QGAVFGEIQLPEGASVNRTAAVTTRIEDIVRNTPGVASVTSVIGYSLIDGLTKSNSAL
                     LIVSLNPFDERKTEALSANGIIARLTGQFQGVQEAVAFAYNLPPIIGLGTGSGFEYQL
                     LSQSGASPTDIAAVARGMVFAANQDPALRRVFTTYSASTPQLYLDIDREKVQTLGINL
                     ADVFNALQSVLANAYVNDFNLFGRTWQVNLQGEASERARVDDIYRINVRNKNGDMVSI
                     RAFAEARLILGPQSIIRYNNFRSVTLN"
BASE COUNT         1097 a         1908 c         1809 g         1071 t
ORIGIN      
        1 accatcatca tcgatacgcc caacacctat ctctattatg tgctcggcgg cggccaggcc
       61 gtccgttacg gtatcggcgt cggccgcgac ggcttcacct ggtcgggcgt gcagtcgatc
      121 accaggaagg ccgagtggcc ggattggacc ccgcccccgg aaatgatcgc gcgccagccc
      181 tatctgccgc gccacatggc cggaggcccc ggcaatccgc tcggcgcccg cgcgatgtat
      241 ctcggcggca cggtgtatcg tattcacggc accaacgcgc cggagaccat cggcacccag
      301 gtttcgtccg gtcgcatccg cctcaccaac gaagacgttt ccgatctcta tgcgcgggtc
      361 aacgtcggta ccaaggtagt cgtgcggcca atggaccgcc gcgccgataa tacggacggc
      421 aagcggagct agctcgctga cgctgccagc gaacgacgtt gaatgaaaaa tggcgcttgt
      481 tcagcgccat tcgtggtttc ctgacatgac aaaactgtaa ctgtgcactt ggccccgcgg
      541 gatggcgccc gcgaaacccg ccctgtagac ttgcggcctc aaccagtctg gggggacgat
      601 gcgtctgaag acgatcccgc tcggggtcgc ggtggtcgcg gtctcgtttt tcgtcagcct
      661 gaaggctatg gactggctgt cgccacgcgc cacggtccct gcgccggtgc ttgtcgaatt
      721 gccgccgctg ccccccgccc cgcgcagttc cagcatcgtc gcaccgatcg tgattgcgct
      781 cagcgccatt cgcgacgccg ccgatcgcgg cgcgccgcgt accttcgcag gcaaagccga
      841 taatccggtt tcgcagatcc tgcagaatgc cgacatcggc tggaccgcat cgcgtggacc
      901 gatatcggcg accggcgcgc aggatgtgct gtcgctggca accccgctga cgggaacgct
      961 gaatgcgacc ggttcgctgt cggcgaaggc gacgggcgcg gtcggcgacg cgctcggcag
     1021 cctgctcggc ggcgatgtcg ccaagcggat cggcagcgtg aacatcaagt cgctcaatgc
     1081 ccatgccgag atcaagggca acgtcaccat taccgcgcgg ccaaagctcg ccgcggcctg
     1141 gcggatcgag ccgaatttga ccgcgcaggt gaccttgagc gacaccaatc tttcggtttc
     1201 gggtgcgcgc gtcaatgtcc cggcgcaggt caagccgctg atcgacaaga ccgtcgccga
     1261 tcagctcgac gcggtgcagg cgcggattcg caacgatccc gcattcgaaa acaacgcaag
     1321 gctgcaatgg gccaaggcct gccgatcgat tccgctgcaa agcccgggca catcctcgtc
     1381 gctgccggca ctctggctgg agctgcggcc gacccgcgcg atcgccgcac aaccgcgcat
     1441 cgatgcatct gcggtgacgc tgacgctcgg catcgaggcg gagacaagga tcactccggc
     1501 gcagaccaag cccgactgcc cgtttccggc gacgatcgac atcgtcccgc cgggcccgag
     1561 ccgtatcagc atcggcgtgc cgatcgatct gccgtttacc gacatcaaca aaatcgtgga
     1621 ggcgcagttc gccggccgga gctttccaga agatggatcg ggctcggtcg acgtcaccgt
     1681 caagcgcgcc agcgtcgcgg catcgggcga ccggcttttg atctcgctgc tggtcaaagc
     1741 caaggagaaa aagagtttcg ttggactagg cggcgaggcc aatattcata tctggggcaa
     1801 gccggtgctc gaccaggaac agcagacctt gcggctgacc gacgtcgaac tcgccgtcga
     1861 atcggaggcg gcgttcggcc tgcttggcgc cgcggcgcgc gccgccgtgc cgcatctgca
     1921 aaaggtgctg gcggacaagg ccaccatcga tctcaagcca ttcgccgcga atgcgcaaaa
     1981 caagatcgcc gcggtgatat ccgattttca gaagaacgag gacggcgtgc ggatcgcggc
     2041 ggaaatcacc agcctgcgcc ttgccgacat cgccttcgat tccaaaaccc tgcgcgtgat
     2101 cgccgaagcc gccggcgcga tcaacgtttc catcaccgcg ctgccggggc tgtgagcttt
     2161 gttcgccctc ttccgcgtgg cgggagagcg cggggagcat cgcgcacgca ttacgagcct
     2221 tactgacaac cctcccgttt gctgctaagc atctgtggtc gtagcgatcg gaggctgaga
     2281 tgcagcgtct gtcactcttg cttgcggccg ttgttctttg tgcggtcctc ctcgtcgccg
     2341 gatgtggcga caacaagcaa ccgaatccgg cggtctccgc accgccgccg gccgtgaccg
     2401 tcgtcaaggt caccgccgcg gagatcaaac cagcgaaaac ctttacgggc cggatcgaag
     2461 ccaaggacaa agtcgatctg cgcgtacgcg tcgacggctt cctcgaaaaa cggttgttta
     2521 ccgaaggagc ggatgtcaag gaaggcgagc ttctgttcgt tatcgaaaag gggctgtacc
     2581 aggccgcggt cgatcaggcg aaagccgggc ttgagacggc gcaggccaca ctgaagcttg
     2641 ccgatatcga cgtcgaccgc caaacgcagt tgatgcagcg caatgtcacg gcccaggcga
     2701 cactcgatca gtccaccgcc aggcagggcg aggcccgcgg caacatgctg gcgcagcaag
     2761 ccgcactgga aaaagcggag cttcagctaa gctacaccga tatcaagtca ccgatctccg
     2821 gacgtatcgg tcgatcatcg gtgtcggtcg gcaacttcgt cagcccgtcg aatagcgcgc
     2881 tggccaccat cgtcagtcag gacccgatct acgccagctt cccggtcacg cagcgcgaga
     2941 tgctcgacgt acgcaagcag caagccgcga gtaagggcag cgagtatgtc atctacatcc
     3001 agctcgcgga cggcagccgc tatcccggcg ccggaaaaat cgacttcctc gacaatacgg
     3061 tgaatcaggg gactgacacg gtccaggtgc gagcggtgtt cgccaatccc gaccgcgtgc
     3121 tggttgacgg acaactggtc accgtcgtgg ccgaagaggg caagggcgag agcgcgctgc
     3181 tggtgccgca gcaggcgctg caggtcgatc agaccggtcc ttacgtcctc gtcgtcgaca
     3241 aggacaacaa gattcaaatc cgacgcgtcg agaccgatgt cgcgcgcggg gtgaacattg
     3301 tgatccgcaa ggggctcgcg gcgaatgagc tcatcgttgc cgaaggcatt cagcgggtgc
     3361 gtccaggaca ggttgtcgcg ccgaccgaag ccaagccggg agcgtagaac atgttgtcga
     3421 gcgtcttcat cgatcgcccc cgattagcgg tcgtcatctc gatcgtcatc accgttgccg
     3481 gcctcgtcgc gctctttcaa attccgatcg cgcaatttcc caatatcgtt ccgccgcaga
     3541 tcgccgtcac cgcatcctat gccggcgcca gcgcggaggt cgtcgaggcc acggttgccc
     3601 aaccgatcga ggcacgcgtc atcggcgtcg acaatatgct ctacatgaag tcgaccagcg
     3661 gcaatgacgg cagctatacg ctcaacatca ccttcgcggt ggggacggac cctgacctca
     3721 ataccgtcaa ggtgcagaac cgcgtcggtc tcgccgaggc ccagctgccc gccgaagtgc
     3781 gcgcgcaggg catcagcgtc accaagaaat cctcagcact gctgcaggtt atcgcgctga
     3841 cctcgcctga cggccgctat gaccagctgt tcctcagcaa ttatgcgacc atcaacgtca
     3901 ttgaccagct gaagcgggta cccggcgtcg gcgacgtctc gctgttttca ccctccgact
     3961 atagcatgaa ggtgtggctc gacaccgatc gcctgacgat tttcggcttg gtcccgagcg
     4021 acgttgcggc cgccctaaag tcccagaata tccaggccgc ggtcgggcgc atcggcgcgc
     4081 aacctgcgct gccagatcag caattccagc tcaacatcca gagcaagggc cgcctgtcga
     4141 gcgtcgagga attcggcgcg gttgtggtgc gcgccaatcc ggatggatcg ttcgtacgcg
     4201 tgcgcgacgt cgcccgcgtc gagctcgcgg ggcggctgtc ggaatcgctc ggccgcctgg
     4261 acggccagcc ggcggcggtc atcgggattt accagtcgcc ggggggcaac gcgctcgata
     4321 gcgcgcaacg gatcggcaag attctccaag aactcaaaac ctcgttcccg gaaggtgtcg
     4381 attacaagat cacctacgac accacgattt tcgtcaagga aagcatcaag ggtgtcctgc
     4441 atacgctggt cgaggccttc atccttgttg tcatcgtcgt gtttttgttc cttggcagcg
     4501 tgcgggccac gctgatcccg ctgctggcag ttcccgtggc actgatcggc accttcgccg
     4561 tgatgctcgc gctcggatat tccgccaaca ccgtctcgct gctcgcgctg gtgctggcca
     4621 tcggcatcgt ggtcgacgat gcgatcgtgg tggtggaagc cgtggaggcg cagcttgaaa
     4681 aggatcattc gctgtcgccg gccgaggcat cgcggcgggc gatggccgag atcaccgcgc
     4741 cgatcatcgc catcacgctc gtcctgcttt cggtgttcgt tccggtcgcc ttcatacccg
     4801 gtatttccgg cgaactgttt cgccagttcg cggtggcggt ttcggtgtcg atggtgattt
     4861 cggcgatcaa cgcgctgacg ctgtcgccgg ccctgtgcgc cgttttcctg tcggccagcc
     4921 acggacccaa acgcggcctg atcggctatg tgctgcgtgg aatcgacaag gcgcgcgacg
     4981 gatattcact ggtcgtccac aagaccgtcc gcttcgcggt ttacagcctg ctggtccttg
     5041 tcgcggtcat cgccggcgcc ggctggctgt tcaaggtcac gccgaccggc ttcctgccgt
     5101 cggaggatca gggcgcggtg ttcggcgaaa ttcaactgcc ggaaggcgcc tcggtcaacc
     5161 gaacggcggc ggtcacgacg cgtatcgagg acatcgtccg caacacgccg ggcgttgcca
     5221 gcgtgacctc ggtgatcggc tacagcctga tcgacggcct gacgaaatcg aacagcgcgc
     5281 tgctgatcgt atcgctcaat ccgtttgacg aacgcaagac cgaggcgctg tcggccaatg
     5341 ggatcatcgc gcgattgacg ggccagttcc agggcgttca ggaggccgta gccttcgcct
     5401 ataacctgcc gcctatcatc ggcctcggca ccggcagcgg cttcgaatac cagcttctca
     5461 gccagtccgg cgcgagcccg accgatatcg ccgcggtcgc gcgcggtatg gtgttcgccg
     5521 ccaatcagga tccggcgctg cggcgggtgt tcaccactta cagcgccagc acgccgcagc
     5581 tctatctcga tatcgaccgc gaaaaagtcc agaccctcgg catcaatttg gcggatgtct
     5641 tcaacgccct gcagtcggtg ctcgccaacg cctacgtcaa cgacttcaac ctgttcggcc
     5701 gtacctggca ggtcaacctg cagggcgagg cctccgaacg cgcgcgcgtt gacgatatct
     5761 atcgcatcaa cgtccgcaac aagaatggcg atatggtttc gattcgtgcc ttcgcggagg
     5821 cacgtttgat cctggggcct caatcgatca tccgctacaa caacttccgc agcgtgacgt
     5881 tgaat
//
LOCUS       DASWGM010000706         7267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1759423,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000706 DASWGM010000000
VERSION     DASWGM010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759423"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..893)
                     /locus_tag="VGA15_19825"
                     /pseudo
     CDS             complement(1..893)
                     /locus_tag="VGA15_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
     gene            complement(895..1590)
                     /locus_tag="VGA15_19830"
     CDS             complement(895..1590)
                     /locus_tag="VGA15_19830"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX9589983.1"
                     /translation="MLLTPTELERLTIFTAAELARRRRAKGLKLNYPEAVAIITDEIL
                     EGAREGRSVADLIGHGSTILSTDDVLSGIAAMMNIIQIECVFPDGTKLVTVHEPIRPA
                     PGSQADPEHPGLVTAADGSIDLNAGRRSVKLKARNTGDRPIQIGSHFHFFEVNKAMEF
                     DRAAAFGMRLDIPAGTAVRFEPGAWKEVSLVTFGGRSELTGLNNLTNGLSSDPAIRDG
                     ALERAKRRGFKGS"
     gene            complement(1604..2278)
                     /locus_tag="VGA15_19835"
     CDS             complement(1604..2278)
                     /locus_tag="VGA15_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX9589984.1"
                     /translation="MMLSILQAIWQADGTFPSGSFAFSYGVEGVVARRPQLDGTALTE
                     LTTTIIRQRWATCDRIALVQAFRAAGALHAIAAIDRDVEASTFGGTMREGSRRNGGSF
                     LASHARLGDATALRLRDAVRAGVCLGHIAVMQGAVWQAMGLDEKLAQLASAYGVASGV
                     TAAAVRLGAIGALEGQRVLRGCLPLIETLVSQPVPDDAELSSFVPFLEIAAARHARAD
                     LRLFVN"
     gene            complement(2275..2817)
                     /gene="ureE"
                     /locus_tag="VGA15_19840"
     CDS             complement(2275..2817)
                     /gene="ureE"
                     /locus_tag="VGA15_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX9589985.1"
                     /translation="MLRIDRVLGSRLETAFSEAIHRLEHRGAVDVVNIPVADLDRRRL
                     LATTQAGEELAIALPRHQKLFDGAVLLIDDARAIVVRAATERWMRLEPRSISDAIELG
                     YHAGNLHWRVRFQGEALFVALEGRPEDYTARLGEMIAARRVGVSILDEEVRDGVGEKG
                     APTGDGEIATKDHFHHDGHR"
     gene            complement(2930..3949)
                     /locus_tag="VGA15_19845"
     CDS             complement(2930..3949)
                     /locus_tag="VGA15_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9589986.1"
                     /translation="MSLDYEIYDLGDVKLQRGPTLRDCKLAYKTFGTLSPNKDNVIVY
                     PTWYSGQHYDNEWLIGDGRALDPKKYFIIIPNMLGNGLSSSPSNTPEPYNGPRFPLVT
                     ACDNVRVQHRLVTEKFGIEHIRLVTGWSMGALQTFHWGALYPGMMDLIAPFCGSAKCS
                     RHNFVFLEGVKAALTADAAWKEGWYTEKPQRGLRAAARVYAGWGFSQAFYRQKLDIEA
                     LGYSSLEDFLVAFWEGFFLPKDPNNLLTMLWTWQNGDIGDNELYHGDFKAALGAIKAR
                     AYVMPGQTDLYFPPEDSEFEVAHMPNAKFVPVPSVWGHFAGGPGTNPKDVEFIDAKLK
                     ELLAS"
     gene            complement(4141..5178)
                     /locus_tag="VGA15_19850"
     CDS             complement(4141..5178)
                     /locus_tag="VGA15_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic amidase"
                     /protein_id="HEX9589987.1"
                     /translation="MLHGDISSSNDTVGVAVVNYKMPRLHTKAEVLANAKKIADMVVG
                     MKTGLPGMDLVIFPEYSTHGIMYDSKEMYETASVVPGEETEIFAEACRKAKVWGVFSL
                     TGERHEEHPKKAPYNTLILMNDKGEVVQKYRKIMPWVPIEGWYPGNCTYVSEGPKGLK
                     VSLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVVMAKAMAWANNVYVAVA
                     NAAGFDGVYSYFGHSAIIGFDGRTLGECGEEDYGIQYAQLSKMLIRDARKNGQSENHL
                     FKLVHRGYTGMINSGEGDRGVAACPYEFYNKWIADPEGTREMVESFTRTTVGTDEAPI
                     EGIPNKVAAHR"
     gene            complement(5263..5958)
                     /gene="urtE"
                     /locus_tag="VGA15_19855"
     CDS             complement(5263..5958)
                     /gene="urtE"
                     /locus_tag="VGA15_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEX9589988.1"
                     /translation="MLKLTNVDSYYDRSHILHAVCLDIPIGQVTAVLGRNGTGKTTLL
                     KTLMGLTDRMEGQISLDGTEIGKDPTFRRARAGIAYVPQGREIIPDFTIRENILMGAF
                     ARADGKREIPELVPELFPYLMDNLERAGGVLSGGQQQQLAIARALAADPKIILLDEPN
                     EGIQPSIIEEIEAIIIRLNKEVGLTVVLVEQNVSFARQASHRFAMMEKGRIVVAGAIA
                     ELSDEMVHRHMAV"
     gene            complement(5958..6707)
                     /gene="urtD"
                     /locus_tag="VGA15_19860"
     CDS             complement(5958..6707)
                     /gene="urtD"
                     /locus_tag="VGA15_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX9589989.1"
                     /translation="MAQFALTIDGLGVDFGGFKAVNGVSLTVEDGELRVLLGANGAGK
                     TTLMDLVSGKTKSTQGRVFLHDTDITNWEEHKIARAGVGRKFQIPSIFKDLTVRRNLE
                     VASCRNPGVLANLRFGFSKQAKQDVDHVLDLIGLTEEANRLAAYLSHGQTQWLELGLL
                     ITQNPKVILLDEPTAGMTQAETHKTSEIVNSLKGKHTLIVVEHDMAFVREIAERITVL
                     HLGAVLAEGNVEQIENDPKVRAAYLGSKGIG"
     gene            complement(6713..>7267)
                     /locus_tag="VGA15_19865"
     CDS             complement(6713..>7267)
                     /locus_tag="VGA15_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX9589990.1"
                     /translation="PYIVPTYYFVAIALCLVLAGARLLIETRAGLILQAIRDDQNRAR
                     YLGFDVPLYQTFFFAVSAGIAGLAGMFYVVVSEFASPTFMDLTFSITMVVWAAVGGRS
                     SILGACIGAIVINMISATVSETEGFAEAWKVIIGLIFVLVVLYLPRGLAGLAHDLLDR
                     FLARGKAAPSQHAIEASDASQLAE"
BASE COUNT         1386 a         2287 c         2181 g         1413 t
ORIGIN      
        1 acgacggcag gcaatgcatc tcgccggcga cgcggatgat gtccggcgcg tggccgccgc
       61 cggcaccctc ggtatggtac atgtgaatgg tgcgaccgcc gatcgcctcc agcgtatcct
      121 cgacgaagcc ggattcattc agcgtgtcgg tatggatctg cacttgaaag tcgtgctcgt
      181 cggcaacccg cagacaggtg tcgatcgccg ccggcatcga gccccagtcc tcgtgtagtt
      241 tcaggcccat cacaccggtt tcaagctgtt cgaccagcgg cggcacgcgg tgcgaattgc
      301 cgcgccccag aaagccaaag ttcatcggcc acgcttccgc ggcctgcagc attttcccgg
      361 tgttgaaggg gccgccggaa tcgatcccaa ccgtgattgg tccgagcgaa ccgccgatca
      421 tggtggtgat gccgcttgcc agcgcatgct cgaccaggcc cgcactgtcg aaatgaacgt
      481 gaacgtcgat cgcgcccggc gttgcgatca gcccctcgca gtcgcgcact gttgtgccgc
      541 tggacacgat caggcgcgga tcgacgccgt ccatgatggc gggattgccg gccttgccga
      601 tcccgacgat gcggccatgg cggatgccga gatcgccttt gacgatcccg acgacgggat
      661 cgatcacgac gacattgcac aacaggaaat ccagcgcgcc ctccgcgctg gtgatcccgg
      721 ccatcccgat tccgtcgcga agggttttgc cgccaccgtg caggcatttc gtcgccatag
      781 acagcgtgat ccttctccac aacggccgtc agcgccgtgt cgccaagccg aacggcgtca
      841 cctgtggtcg gaccgtagag tgccgcgtaa tcccggcgat cgatgcttgc catgttacga
      901 ccctttgaag ccccgcctct tcgcccgttc aagcgccccg tcacgtatgg caggatccga
      961 gctcaatccg ttggtaaggt tgttcagccc ggttagttcg ctgcggccgc cgaatgtcac
     1021 gagcgagact tccttccagg cgccgggttc gaaccgcacc gcggtgcccg cgggaatatc
     1081 gagccgcatg ccgaatgcgg cggcgcggtc gaattccatg gccttgttga cctcgaagaa
     1141 atggaagtgc gaaccgatct ggatcggccg gtcgccggtg ttacgcgcct tcaatttgac
     1201 cgagcggcgc cccgcattga gatcgatgct gccgtcggct gcggtgacga ggccgggatg
     1261 ttccggatca gcctgcgaac cgggcgccgg ccggatcggt tcgtggactg tcacgagctt
     1321 ggtaccgtcg ggaaacacgc actcgatctg aatgatgttc atcatcgcgg cgatgccgga
     1381 cagcacgtca tcggtcgata gaatggtcga tccatgcccg atcagatcgg caaccgatcg
     1441 cccctcccgc gcgccttcga ggatttcgtc ggtgatgatg gcaacagctt cgggatagtt
     1501 caacttgagc cccttggcac gccgccggcg cgccagctcg gccgcggtga agatcgtcaa
     1561 gcgctcgagc tcggtgggcg tcagcagcat cgttcacaac ctctcaattg acgaacaggc
     1621 gaaggtcggc gcgcgcgtgc cgcgcggcgg caatctcaag aaatggaacg aagctcgaaa
     1681 gttcggcatc gtccgggacc ggctggctaa ccagcgtctc gatcaaggga aggcatccgc
     1741 gcagaaccct ctggccctcc agtgcgccga tcgcgccaag ccgcaccgcc gcggctgtca
     1801 caccggaggc gacaccatag gcggaagcga gttgcgccag tttttcgtca agacccatcg
     1861 cctgccagac cgcaccctgc atcacggcaa tgtggccaag gcagacgccc gcgcgaactg
     1921 cgtcgcgcaa tcgcagcgcc gtggcgtcgc cgagccttgc atgcgacgcc aggaaggatc
     1981 cgccgttacg ccgcgagcct tcgcgcatcg tgccgccgaa cgtcgaggcc tccacgtcac
     2041 ggtcgatcgc tgcaatggcg tgaagggcgc cggcggcgcg gaacgcctgt accagcgcga
     2101 tccggtcgca cgtcgcccag cgctggcgaa tgatcgtggt cgtcaattcg gtaagcgcgg
     2161 tcccatccag ttggggacgc cgggcgacaa cgccctcgac accataagag aaagcgaacg
     2221 atccgctcgg aaacgttcca tcggcttgcc agatcgcctg caggatcgat aacatcatcg
     2281 atggccatcg tggtgaaaat gatctttcgt ggcgatctcg ccgtcacctg tcggcgcgcc
     2341 tttttcaccg actccgtcgc gcacctcttc atccagtatg ctgacaccga cgcggcgcgc
     2401 ggcgatcatc tcgccgagac gcgcggtgta gtcctcgggc cggccttcca gcgcaacgaa
     2461 caaggcctcg ccctggaaac gcacgcgcca atgaagattc ccggcgtgat agccgagttc
     2521 gatcgcatcg ctgatcgagc gcggttcgag gcgcatccat cgctccgtgg cggcgcgaac
     2581 gacgatcgcg cgcgcgtcgt cgatcaagag gaccgcgccg tcgaacagct tctggtgacg
     2641 cggcaacgcg atcgccagtt cttcgcccgc ttgcgtcgtc gccagcagcc ggcgacgatc
     2701 gaggtcggcc accgggatat tgaccacgtc caccgcgccg cgatgttcga ggcggtgaat
     2761 cgcctccgag aacgcggttt ccaggcggct gcccaaaaca cggtcgatgc gcagcatcgc
     2821 gtgaggccct ttcaacggtt agaccggtga agaatgactg atgctcagca tgctcagata
     2881 tccttcctgg gaaaatacgg gaccggaaga ttaattccgg cgccggcgct tacgatgcaa
     2941 gaagctcctt tagcttcgca tcgatgaatt cgacatcctt cggattggtg ccgggtcccc
     3001 cggcgaagtg gccccagaca gacggaaccg ggacaaattt ggcgttcggc atatgcgcga
     3061 cctcgaactc gctatcttcc ggaggaaaat acaaatcggt ctggccaggc atgacatagg
     3121 ccctggcctt gattgcgccg agcgccgcct tgaagtcgcc gtggtaaagc tcgttgtcgc
     3181 cgatgtcccc gttctgccag gtccagagca tggtcagcag gttgtttgga tccttcggca
     3241 ggaagaaccc ttcccagaac gccaccagaa aatcctcaag cgaggagtag cccagcgcct
     3301 cgatatccag tttctggcga taaaacgcct gcgaaaagcc ccagccggca tagacccgcg
     3361 ccgccgcgcg caacccccgt tgcggcttct cggtgtacca gccttccttc catgccgcgt
     3421 cagcggtaag cgcggccttg acgccctcca ggaaaacgaa gttgtgacgt gagcatttcg
     3481 ccgaaccgca gaatggcgcg atcaggtcca tcatgccggg atagagcgcg ccccagtgaa
     3541 aggtctgcag cgcgcccatc gaccagccgg tcacgagccg gatatgctcg atgccaaact
     3601 tctcggtgac gagacggtgc tgcacgcgca cattgtcgca ggccgtcacc agcggaaagc
     3661 gcgggccatt gtagggctcc ggcgtattgc tcggtgacga tgacaggccg ttgccgagca
     3721 tatttggaat aatgatgaag tatttcttcg gatcgagcgc acggccatcg ccgatcagcc
     3781 attcattgtc gtagtgctgg cctgaatacc aggtggggta cacgattacg ttgtccttgt
     3841 tcgggctgag cgtgccgaag gttttgtaag ccagcttgca gtcgcgcagc gtcgggccgc
     3901 gctgcagctt gacgtcgccc agatcgtaga tttcgtagtc gaggctcatg gcgaatgtcc
     3961 ttgttttttg gttcatgccg gaaaaacgga tcggcctccg aaagggccga ccgacgctga
     4021 cgccccgcaa tttcgcaggg atcgccaccg cagccaggac tccaatccgg ataagcgaac
     4081 tcctccctac cccttgcgcg ccggcgcaag aacgccggcg cgcaagcttc cagcctggac
     4141 tcagcgatga gcggcgacct tgtttggaat tccctcaatc ggagcttcgt cggttcccac
     4201 cgtggtccgg gtaaaggatt ccaccatttc gcgcgtccct tcgggatcgg cgatccattt
     4261 gttgtagaac tcataggggc atgccgcgac gccgcggtcg ccttcgccgg aattgatcat
     4321 tccggtataa ccgcggtgca ccagcttgaa gagatggttt tcggattgtc cgttcttgcg
     4381 tgcgtcgcgg atcagcatct tcgaaagctg ggcgtactga atgccgtaat cctcctcgcc
     4441 acactcccca agcgtgcggc catcaaaccc gatgatcgct gagtggccga aataggagta
     4501 gacgccgtcg aagccggcgg cgttggcgac ggccacatag acgttgttgg cccacgccat
     4561 cgccttggcc atcaccacct gctgatcctt tgccggatac atgtagccct gacagcgcac
     4621 gatcagttcg gcgcccttca tcgcgcagtc gcgccatatc tccggatagt tgccgtcatc
     4681 gcagatgatc agcgaaacct tgaggccttt gggcccctcg gagacatagg tgcagttgcc
     4741 gggataccag ccctcgatcg gcacccacgg catgatcttg cgatatttct ggaccacttc
     4801 gcccttgtcg ttcatcagga tcagcgtgtt gtagggcgcc ttcttcggat gctcctcgtg
     4861 acgttcgccg gtgagcgaaa acacccccca gacctttgcc ttgcggcagg cctccgcgaa
     4921 aatctcggtc tcctctccag gcacgaccga tgccgtttcg tacatctcct tggagtcgta
     4981 catgatgccg tgggtcgaat attccgggaa aatcaccaga tccattcctg gcagaccggt
     5041 tttcatgccg accaccatat cggcgatctt cttggcgttg gccagaacct ccgccttggt
     5101 gtggaggcga ggcattttgt aattcacgac ggcgacgccg accgtgtcat tgcttgatga
     5161 gatatcgccg tgcagcatgt tgctctcctt gcggttggtt gacggttaga aacttttctg
     5221 ttcgtcggcg tttgtacggg ctggctccca caatgatccg catcacaccg ccatgtgccg
     5281 gtggaccatt tcatcgctca gttcggcaat cgcgccggcc accactatcc gtcccttctc
     5341 catcatggcg aagcgatgcg atgcctggcg cgcaaacgag acattctgct cgaccagcac
     5401 gacggtcagg ccgacctcct tgttgaggcg aatgatgatg gcttcgattt cctcgatgat
     5461 tgacggctgg atgccttcgt tcggctcatc gagcaggatg atcttcggat cggcggcaag
     5521 cgcccgcgcg atcgcgagct gctgctgctg gccaccggaa agcacgccac cggcgcgttc
     5581 gagattatcc atcagatagg gaaacagctc cggtaccaat tcgggaattt cccgcttgcc
     5641 gtcggcgcgg gcgaaagcgc ccatcaggat gttctcccgg atggtgaaat cgggaatgat
     5701 ctcacggccc tgaggaacat aggcaatgcc ggcgcgggcg cggcggaagg tcggatcttt
     5761 tccaatctcg gttccgtcga gactgatttg tccttccatc cggtcggtaa gacccatcag
     5821 tgtcttgaga agggtggtct ttccggtgcc attgcggccg agcaccgcgg tcacctgccc
     5881 gatcggaata tccaggcaca ccgcatgcag aatgtggctg cggtcatagt agctgtcgac
     5941 gttcgtcagc ttgagcatca gccaatcccc ttcgatccaa ggtaggctgc gcgaactttc
     6001 ggatcgtttt cgatctgctc gacgttgccc tccgcgagaa ccgcaccgag gtgcagcacg
     6061 gtgatgcgtt cggcgatctc ccgcacgaac gccatatcgt gctcgacaac gatgagggtg
     6121 tgcttgccct tgaggctatt gacgatctcc gaggtcttgt gggtttccgc ctgcgtcatg
     6181 ccggcggtcg gctcgtcgag caggatcacc tttggatttt gcgtgatcaa aagacccagt
     6241 tcgagccact gcgtttggcc gtggctgaga taggcggcca accggttcgc ctcttcggtc
     6301 agaccgatca gatcgagcac atggtcgaca tcctgtttcg cctgcttgct gaagccaaag
     6361 cgcagattgg ccagcactcc cggatttcgg cagctcgcga cttcgagatt gcggcgtacg
     6421 gtcaggtcct tgaagatgct cggaatctgg aactttcgtc cgacgcccgc gcgcgcgatc
     6481 ttgtgctcct cccagttggt gatgtcggtg tcgtgcagaa agacgcggcc ctgtgtgctt
     6541 ttggtcttgc cggacacgag gtccatcaag gtggtcttgc cggcgccgtt ggcgccgagc
     6601 aggacgcgca gttcgccatc ctcgaccgtc agcgacacac cattgacggc cttgaacccg
     6661 ccgaagtcga caccaaggcc atcgatggta agcgcgaact gagccatccc gctcactccg
     6721 ccagttgcga ggcgtcggaa gcctcgattg cgtgttgcga tggggccgcc ttgccgcggg
     6781 cgaggaaccg atcgagcaga tcatgcgcca gcccggcaag accgcgcggc agatacagca
     6841 cgacgagaac gaagatcagt ccgatgatga ccttccacgc ctcagcgaaa ccttcggtct
     6901 cgctgacggt ggccgaaatc atgttgatca cgatggcgcc gatacaggcg ccgaggatcg
     6961 acgaacggcc gccgaccgcg gcccagacca ccatcgtgat cgaaaaggtg aggtccatga
     7021 aggtcggcga ggcgaactcc gacaccacga cataaaacat cccggccaga cccgcgattc
     7081 ccgccgacac cgcgaagaaa aacgtctgat aaagcggcac gtcgaagccg aggtaacggg
     7141 cgcgattctg gtcgtcgcgg atcgcctgca ggatgaggcc ggcgcgggtc tcgatcagca
     7201 atcgcgcgcc tgcgagaacc aggcacagcg cgatggcgac gaaataataa gtcggaacga
     7261 tataggg
//
LOCUS       DASWGM010000707         6399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1444101,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000707 DASWGM010000000
VERSION     DASWGM010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444101"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /gene="fliF"
                     /locus_tag="VGA15_19870"
     CDS             complement(<1..561)
                     /gene="fliF"
                     /locus_tag="VGA15_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX9589991.1"
                     /translation="MQGLAGFLKGLGAARLMAMIAVTAALISFFGFVIMRVTTPQMTT
                     LFTDLSTEDSSGIIKDLERQAIPFELRNDGAVIMVPKDKVTRLRMKLAEGGLPKGGGV
                     GYEIFDKSDALGTTSFVQNINHLRALEGELARTIRAIDRVQAARVHLVLPERPLFSRE
                     TPEPSASIVVRVRGALEPQQIRAIRHL"
     gene            complement(738..1013)
                     /locus_tag="VGA15_19875"
     CDS             complement(738..1013)
                     /locus_tag="VGA15_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEX9589992.1"
                     /translation="MTEPHRPRVKYVIGPDGSPLTIADLPAPGTKRWVIRRKAEVVAA
                     VRGGLLSLEEACSRYTLTVDEFLSWQFSIDQHGLAGLRTTRIQQYRQ"
     gene            complement(1214..1912)
                     /locus_tag="VGA15_19880"
     CDS             complement(1214..1912)
                     /locus_tag="VGA15_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEX9589993.1"
                     /translation="MTTTAATNVASPIVSGTTPAPASSGSSGSGSTSNTTLAGNFQTF
                     LTLLTTQLQNQNPLDPLDTNQFTQQLVQFAGVEQQLKTNDQLTTLVSLQKTAQSTQAL
                     GFVGKTAVVDGSTATMTKGAASWLLGVKTNSNVNISITNSTGQTVFSGNYPVTAGDNQ
                     AFNWDGKGNDGTQWPDGNYKLTATAADSAGNSVAVSTEILGVVSSVDLTQSPPLLSIN
                     GQNYTVDQIKGITG"
     gene            complement(1923..3509)
                     /locus_tag="VGA15_19885"
     CDS             complement(1923..3509)
                     /locus_tag="VGA15_19885"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX9589994.1"
                     /translation="MNSNTTADTGSGRAPEPSASQRRSDDTSAAADSRRSRDTSTADQ
                     TARTDSGGRDAAATQRSDANANADASSNADASANADAAQRSRAKSDTSKTDDTKSIAK
                     PSSNDSPAADSKANPANPAQQDGPGATTPTPVAVVIPVAVASTIVPAASPSSGKATAP
                     LAIAAAAIAASSAATGAPAASAAQAETDSGAAVTAAATAPAAAAAAAAGTKIEVQTAA
                     SAAAVSVNRPATQSDAIVATDATLATAGAATKPATPKSALPKAPAAAKAGAAPSTDSA
                     AAPGTPEPSATAISTGTAHTDVAPQPASAGKEKAENSIVDAAKTDAAAGSSSTPAAIV
                     AAHQPSAIPEAGQALTGSSDAGAQATTAIQPQLPAAAAVAAPVGPFSVTAATNAAVPL
                     SGVALEIAASARSGKSRFEIRLDPADLGRIDVRIDVDRSGQVTSHLTVEKPETLSMLR
                     QDAPQLQRQLDDAGFKTASGGLQFSLRDQSSSNQNNGDETGRNAQRLVVSSEEPTPAA
                     IVGRTYGRILGSSSGVDIKV"
     gene            3988..5193
                     /gene="mnmA"
                     /locus_tag="VGA15_19890"
     CDS             3988..5193
                     /gene="mnmA"
                     /locus_tag="VGA15_19890"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX9589995.1"
                     /translation="MLNSLDLEGRPQDTRIVVAMSGGVDSSATAALLKSEGYDVVGIT
                     LQLYDHGAATHRKGACCAGQDIHDARNVAERIGIPHYVLDYEDRFRQSVIDNFADSYA
                     SGETPVPCIECNRSIKFRDLLKTARELGASALATGHYVASRRLSDGSRALTCAADADR
                     DQSYFLFATTREQLDYLRFPLGDMTKPQARELARRFGLSVADKQDSQDICFVPTGRYT
                     DIIGRLRPNAVLPGDIVDLDGRVIGTHQGIVHFTVGQRRGLGIASSAPLYVVRLDAVG
                     RRVVVGPREALRMDRIVLRDVNWIGDGALDRAVGEGLEIFVRVRSTRAPQPAWLRAAD
                     GGYEVELVAGEEGVSPGQACVFYDAPSGQARVLGGGFIQSATAKSTTAGVAARGSLAQ
                     PSLVEVMRG"
     gene            5211..5849
                     /locus_tag="VGA15_19895"
     CDS             5211..5849
                     /locus_tag="VGA15_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9589996.1"
                     /translation="MASDIDRAEVAKAYGRWAPVYDLVFGKVFDHGRQSTIAEADKIG
                     GRILDVGVGTGLSLSDYSRTTKLCGVDISEPMLRKAQQRVRAFGLGNVETLAVMDAKS
                     LAFADSSFDAVVAQYVITAVPDPEATLDDFIRVLKPGGELILVNHIGAESGPRRIFEL
                     AFAPLARRLGWRPEFPWARLTDWAAKHGGVSLTERRPMPPMGHFSLIRYRKS"
     gene            5902..6264
                     /locus_tag="VGA15_19900"
     CDS             5902..6264
                     /locus_tag="VGA15_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589997.1"
                     /translation="MRMKRSLFLSSLLMAASGMAAAQAPPVPATPPAQTAPPAPARAS
                     DCAPMQSMPQGNIAPQGTTTGQSIEPLGDKLAKSDGVLCPPAGIDPQMRAPTPDTGNT
                     PVIPPPGSPGGDPTVRPK"
BASE COUNT         1059 a         1952 c         2134 g         1254 t
ORIGIN      
        1 cagatggcgg atggcacgga tctgctgcgg ctcgagcgcg ccgcgcacgc gcaccacgat
       61 cgaggccgat ggttcgggcg tctcgcgcga aaacagcggc cgctcgggca gcaccagatg
      121 gacgcgggcg gcctggacgc ggtcgatggc gcggatggtg cgggccaatt cgccttcaag
      181 cgcgcgcaaa tgattgatgt tctggacaaa gctggtggtg ccaagcgcgt cggacttgtc
      241 gaaaatctcg tagccgacgc cgccgccctt gggcaatccg ccctcggcga gcttcatgcg
      301 cagccgggtg accttgtctt tcggcaccat gatcacggcg ccgtcgttgc gcagctcgaa
      361 ggggatcgcc tggcgttcca ggtccttgat gatcccggag gaatcttcgg tgctgaggtc
      421 ggtgaagagg gtggtcatct gcggcgtggt gacgcgcatg atgacaaagc cgaagaagct
      481 gatcagcgcg gctgtcaccg cgatcatcgc catcagccgc gccgcgccca gccccttcag
      541 gaaacctgca agaccctgca aaaccaaacc cgcccctgag attcggccgc aagcgccgac
      601 tgggcaatta ttgcctatgg gatggtttcg atatggttaa cgcaggttaa gagcgacggc
      661 gaaagttcaa gatgggaaag atcggcttcg cgaaacgaag ccgatcttca tcgaaagccg
      721 tcggcgccgg aaagcgttta ttgacgatat tgctggatgc gggtggtgcg cagtccggcc
      781 aggccgtgct ggtcgatgga aaactgccag gacaggaatt catcgacggt cagcgtgtaa
      841 cggctgcaag cctcctcgag ggagagcagg ccgccgcgca ctgcggcaac gacttcggcc
      901 ttacggcgaa taacccaccg tttggtcccg ggcgcgggca gatccgcaat tgttaacgga
      961 ctgccgtcag gcccgatgac gtattttacc ctcgggcgat ggggttctgt catggcgtac
     1021 tcacaaactc tcgaccactg aactcacggt aacaacctac gccccacgac ttaaaaattg
     1081 cctaagcccg acgctgcaat gagaatctcc ttgaaattgg ctgcaaaacc tttggcccgg
     1141 cgtctgaagg acgccgggcg tgaagcagac cgtgaatgaa tcgttaattt atccgaggct
     1201 cgatcgcctg ggattagccc gtgatgccct tgatctggtc gacggtgtag ttttggccat
     1261 tgatcgacag caatgggggc gactgggtga gatccaccga actgaccacg cccagtatct
     1321 cggtggagac ggccacggaa ttgcccgcgc tgtcggcagc tgtggcggtc agcttgtagt
     1381 tgccatccgg ccattgcgtg ccgtcgttac ccttgccgtc ccaattgaag gcctgattgt
     1441 cgccggcagt gacggggtag ttgccggaga agacggtctg cccggtgctg ttggtgatgg
     1501 agatgttcac gttggaattt gtcttaactc ccagaagcca gctcgctgct cctttagtca
     1561 tggtcgcagt gctgccgtcg accaccgcgg tcttgccgac aaaaccgagc gcctgcgtcg
     1621 attgggcggt tttctgcaac gagaccagcg tggtcagttg atcgttggtc ttgagttgct
     1681 gttccacccc tgcgaactgt accagctgct gggtgaactg gttggtgtcc agcggatcga
     1741 gcgggttctg gttttgcaat tgcgtcgtca acagcgtcag aaacgtttga aagtttccgg
     1801 caagcgtcgt gttgctggtc gaaccggacc ccgaagagcc ggatgatgct ggagcgggtg
     1861 ttgtccccga aacgatcggg ctggccacgt ttgttgctgc agttgttgtc atgtccgtct
     1921 cctcagacct tgatgtcgac gccgctgctc gatccgagga tgcggccata ggttcgtccc
     1981 acgatcgctg cgggggtggg ttcttcgctg ctgacgacca gccgctgcgc gttgcggccg
     2041 gtttcgtcgc cgttgttctg gttggacgag gactggtcgc ggaggctgaa ttgcaggccg
     2101 ccgcttgcgg tcttgaagcc ggcgtcatcg agctggcgct gcagctgggg tgcatcctgg
     2161 cgcagcattg acagcgtttc cggtttttcg actgtcaaat gcgaggtcac ctggccgctg
     2221 cgatcgacat cgatgcgaac atcgatgcgg ccgagatcgg cgggatcgag cctgatctcg
     2281 aaacggctct tgccgcttcg tgccgaggcg gcgatctcga gtgcaacccc gctcaacggc
     2341 acggcggcat tggttgccgc cgtgacgctg aagggtccga ccggagcggc cacggccgcg
     2401 gcggcgggga gctgcggctg gattgcggtt gtcgcctgcg cgcccgcatc agatgaaccg
     2461 gttagcgctt gtcccgcctc ggggatcgcc gaaggttgat gcgccgccac aatggcagcc
     2521 ggcgtcgatg aggagccggc ggcggcgtcg gttttcgccg catcaacaat gctgttttcg
     2581 gccttctcct tgccggcgct ggccggttgc ggagcgacgt cggtgtgggc ggtcccggtc
     2641 gatatggccg ttgcggaagg ttcgggcgtg cccggcgcgg cggcggaatc ggttgatggc
     2701 gcggcacccg cttttgcggc ggctggcgcc ttggggagcg cggatttcgg cgtagcaggt
     2761 tttgtcgcag caccggcggt ggccaatgtc gcatcggtcg ccacgattgc atcggactgc
     2821 gttgccggcc gattcaccga cacggcggcc gcgctagccg cggtctgaac ctcgatcttt
     2881 gtgccggcgg ccgcagccgc ggcagctgcc ggcgcggtcg ctgctgccgt taccgctgcg
     2941 ccggaatcgg tttcggcctg ggctgcggat gcagccggtg cgccggtggc cgccgagctg
     3001 gcagcgattg ccgctgcggc gatcgccagc ggcgcggtgg ccttgccgga cgatggcgaa
     3061 gctgcgggaa cgatcgtcga tgcgacggcg accggaatca caaccgcgac cggggtcggc
     3121 gtcgtcgctc ccgggccgtc ctgttgcgcc gggttggcag ggttggcttt ggaatcggct
     3181 gccggactat cgttggaaga tggctttgcg attgatttcg tgtcgtctgt tttcgaagta
     3241 tcggatttcg cccgggagcg ctgggccgca tcggcgttcg cgctagcatc ggcattggag
     3301 ctagcatcgg cattggcgtt ggcatcggaa cgctgtgtgg cggcggcgtc gcgaccgccc
     3361 gaatcggttc tcgctgtctg atcggcggtc gaggtgtcac gcgatcgtct gctgtccgca
     3421 gccgccgagg tgtcgtcgga gcggcgttgc gacgcggatg gctccggcgc gcgaccgctg
     3481 ccggtgtcgg cggtcgtgtt gctgttcacc agtgccgcga aacgatcgtt tccgggcggt
     3541 tggtcggacc gcgcaggcct cggtggcggc gggccctgaa aggacaggct ggttgctgct
     3601 tctgacgtcg aactgtacac gggccggcct cgcgcaatga aactggaact aaggtcagca
     3661 aggaccgggc catcgcgttt ctcaaaaaat actcagattt ttcagtatct tactaggatg
     3721 cccggcgaac tacggagcag ccatgagtcc cccttttcct gcccagcggc aagatttgcc
     3781 ggttgcggcg cccaaaacga gttcggctga ttgcctcgcc ggaataccgc ctatattaaa
     3841 gagcagcttt gcagccattc cgatccggcg aacgcgctcc cgggaccgct gcaaggcctt
     3901 tgaccgacaa ggctttttca ctattcgcaa aatggcggct tgatcgcgaa gcgctcgatg
     3961 ccgccgccat cgcaaccggc cggaaccatg ctcaacagtc tggatctcga agggcgtcca
     4021 caggacacca ggatcgtggt cgccatgtcc ggcggagtcg attcgtcggc gacggctgca
     4081 ttgttgaaat ccgagggcta cgacgtcgtc ggcatcacgc tgcagcttta cgatcatggc
     4141 gccgccaccc atcgcaaggg cgcctgctgc gctgggcagg acatccatga cgcgcgcaat
     4201 gtcgccgagc ggatcggcat tccgcattac gtgctggact acgaagaccg ttttcgtcaa
     4261 tcggtgatcg acaatttcgc cgacagctat gcttccggcg agacgccggt gccttgcatc
     4321 gaatgcaacc gctcgatcaa gtttcgcgat ctgctcaaga ccgcgcgcga actcggcgct
     4381 tcggcgcttg cgaccggaca ctatgtggcg tcgcgccgcc taagcgatgg atcgcgtgcg
     4441 cttacttgcg ccgccgacgc cgatcgcgac cagagctatt tcctgttcgc gaccacgcgc
     4501 gagcaactcg actatctgcg ctttccgctg ggcgacatga ccaagccgca ggcgcgcgaa
     4561 ttggcgcggc gcttcggcct gagcgtcgcg gacaagcagg acagccagga tatctgcttc
     4621 gtgccgaccg ggcgctacac cgacatcatc ggccggttgc ggccgaatgc ggttctgccc
     4681 ggcgatatcg tcgacctcga cggccgcgtc atcggcacgc atcagggcat cgttcatttc
     4741 accgtaggcc agcggcgggg attgggcatc gcctccagcg cgccgctgta tgtggtgcgg
     4801 ctcgacgccg tcggccgccg cgtcgtggtg ggcccgcgcg aggcgttgcg gatggatcgc
     4861 attgtgctgc gcgatgtcaa ctggatcggc gacggcgcgc tcgatcgcgc ggtcggcgag
     4921 ggtctcgaga ttttcgtgcg cgtgcgatcg acccgggcgc cgcagccggc ctggctgcgc
     4981 gcggccgacg gcggttatga agtcgaactc gtcgccggcg aagagggcgt ttccccgggc
     5041 caggcctgcg tgttctacga tgcgccctcg ggccaggcgc gcgtgctcgg cggcggattc
     5101 atccagagtg cgaccgcgaa gagcacaacg gccggggtcg cggcgcgcgg ttccctcgcg
     5161 cagccttcgc tcgtcgaggt catgcgcgga taagatttca gggcgggggc atggcttcag
     5221 atatcgaccg cgcggaggtc gcaaaggctt atgggcgctg ggcaccggtc tacgatctgg
     5281 tattcggcaa ggttttcgat cacggccgcc aatcgaccat tgcggaagcc gacaagatcg
     5341 gcggacggat tctcgatgtc ggcgtcggca ccggattgtc gctgtcggat tattcgcgga
     5401 ccacgaaatt gtgcggcgtc gatatttccg agccgatgct gcgcaaggcg caacagcgcg
     5461 tgcgcgcgtt cggtctcggt aatgtcgaga cgcttgcggt catggacgcc aagagtttgg
     5521 ccttcgcgga ttcgtctttc gacgcggtgg tggcgcaata tgtcatcacc gcggtgcccg
     5581 atcccgaagc cacgctggac gatttcatcc gcgtgctgaa acccggtggc gaactcattc
     5641 tggtcaacca catcggcgcc gagagcggcc cgcgccgcat cttcgaactg gcgttcgcgc
     5701 cgctggcgcg gcggcttggc tggcggccgg aatttccgtg ggcgcgcctg acagactggg
     5761 ccgccaaaca cggcggcgtt agcctgaccg agcgccgccc gatgccgcca atggggcatt
     5821 tttcacttat tcgctaccgc aaatcctgag attaccgcga gcttatgcgg aacatccgcc
     5881 cgtgcagacc gttttagccg catgcggatg aaacgctcac tcttcctttc cagccttctg
     5941 atggcggcgt ccggcatggc ggctgcgcaa gcccctcctg tgccggcgac gccgccggcg
     6001 caaaccgccc cgcctgcgcc cgcccgcgcc agcgattgcg cgccgatgca atcgatgccg
     6061 cagggaaaca tcgccccgca gggcacgacg accggtcaaa gcattgaacc actgggcgat
     6121 aaactcgcca aatccgacgg cgtgctgtgc ccgcccgcgg gcatcgaccc gcagatgcgg
     6181 gcaccgacgc cggataccgg caatacgccg gtgataccgc cccccggcag ccccggcggc
     6241 gatccgaccg tccgtccaaa atagcacgct ttacaatcac ttggcggaaa gggcgggttt
     6301 gcttgacatg cccaagacct gcttcttata tgccgcgcgt tcgcgactgc ggtttatgag
     6361 ccttgggacg atgaacgccg tggcggggta gctcagctg
//
LOCUS       DASWGM010000708         3830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_347075,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000708 DASWGM010000000
VERSION     DASWGM010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347075"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VGA15_19905"
     CDS             <1..598
                     /locus_tag="VGA15_19905"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9589998.1"
                     /translation="EAMDPEVLAWDDASNNRCLNAGKCIAIHNPISAFESAKKDKILV
                     PGTQQPIAEVIDHINTPRGPKDRKATTAFFCVGIWNFSKNVELAKDFLRFHFQPENQA
                     KWVEAGHGFNMPFLNNLTNHPVYRSDPQYRHIPDVAKVTVPPSWPGPTTAASQQVWDL
                     YIIPDMFAQYATGRMTLDQAIGWGEKEMLAIYNSKRRT"
     gene            647..877
                     /locus_tag="VGA15_19910"
     CDS             647..877
                     /locus_tag="VGA15_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9589999.1"
                     /translation="MPVSFFGLYRGDGIATEDSLFGRRGNNPYSHFRVLKLPERGTHE
                     RTLMLGCVIALRYLGRLGVNRVVLTVCRSVPV"
     gene            complement(914..1498)
                     /locus_tag="VGA15_19915"
     CDS             complement(914..1498)
                     /locus_tag="VGA15_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX9590000.1"
                     /translation="MIRLLFTIIAGALLGGVVHLVSVLALPRIATQDAYSRLTPITTL
                     NSVIALPLADPNNALMPFMDPAFAIAICCYDLSAGPIKLAVPVSQAYTSVSFYTRNEV
                     AYYAINDRSAGRKVIELDLMTEAQHADLPEDEDVTAADRLIIDSPTATGLIVLKALAP
                     EPGLMPQAQGSLAASSCKVQSEPPAKQAEPAAKR"
     gene            complement(1495..2073)
                     /locus_tag="VGA15_19920"
     CDS             complement(1495..2073)
                     /locus_tag="VGA15_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX9590001.1"
                     /translation="MRLIFITLLALILATVVGLGATWVTATRGTDLGTLTIGAWTARP
                     KTGTADVDPYSRASIARSGELPVGTGDGVAFSATTDDRKRLLDGRCDVVVSGVTPAAR
                     FWTLTLYDSKGHLVANALQRYGFTSQEIIRGADGAFEIRVASRSRAGNWLPTGGIERY
                     ALMLRLYDTPVGVATRSQRDAPMPSVATVGCP"
     gene            complement(2076..>3830)
                     /locus_tag="VGA15_19925"
     CDS             complement(2076..>3830)
                     /locus_tag="VGA15_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX9590002.1"
                     /translation="LAKNLFLSNERTIERKVNEAFLAVWLETRLSKNEILKLYLDRAY
                     MGGGTFGVDGAAHFYFNKSARDVNLAEAAMLAGLFKAPTKYAPHINLPAARARANVVL
                     DNLVDAGFMTEGQVFGARRNPATAVDRRDENSPNYYLDWAFDEMRKLVDTFPRSYVER
                     VFVVRTAIDMNVQRAAEEAIENQLRQFGRDYHATQAATVVADLDGGVRAMVGGRDYGA
                     SQFNRAVDAYRQPGSSFKPYVYSTALMNGFKPTSIVVDGPVCIGNWCPQNYGHSYSGA
                     VTLTQAITRSINVVPVKLSIAIGGKGGPKAGRAKIVEVARRFGIKAPLPDTPSLPIGA
                     DEVTVIEHAVAYATFPNKGKSVTPHSVLEVRTGTGDLVWRFDRDGKKPLQAIPASVAS
                     DMAGMMSHVVSEGTARRAALDGIPTAGKTGTTNAYRDAWFVGYTGNFTCAVWYGNDDY
                     SPTNRMTGGSLPAQTWHDIMVAAHQGVEIKEIPGIGMGTKLPAPPVSATIAANGAPKP
                     PDIKPGPPPILTKRGADILVRVEKMLDEAGRAAGKTSSGDPVKPLKPASSSSVAFPES
                     FAAAVPGTPAAMPVPRKN"
BASE COUNT          682 a         1220 c         1244 g          684 t
ORIGIN      
        1 cgaggcgatg gaccccgagg tgctggcctg ggacgacgcc agcaacaacc gctgcctcaa
       61 cgccggcaaa tgcatcgcca tccacaaccc gatcagcgcg ttcgaatcgg cgaaaaaaga
      121 caagatcctg gtgcccggca cgcagcagcc gatcgccgaa gtaatcgacc acatcaacac
      181 gccgcgaggt ccgaaggacc gcaaggccac aacagctttc ttttgcgtcg gcatctggaa
      241 cttctcgaaa aacgtcgagc tagccaagga cttcctgcgg ttccacttcc agcctgagaa
      301 ccaagccaaa tgggtggaag ccggccacgg cttcaacatg ccgttcttga acaacctgac
      361 caaccatccg gtttacagga gcgacccgca gtaccggcac attcccgacg tggccaaggt
      421 tacggtcccg ccgtcctggc cagggccgac caccgcagcc tcgcagcagg tctgggatct
      481 ctacatcatc cccgacatgt tcgcgcagta cgcgaccggc cggatgacgc tcgaccaggc
      541 gatcggctgg ggcgagaaag aaatgctggc tatctacaat agcaagcggc ggacctgacc
      601 gttcgcgccg ggtcggcctg gggtgcggct gccgacccgc gcgctaatgc ccgtgtcatt
      661 tttcgggctc tatcgcggcg acggcatcgc gaccgaggat tcgctgtttg gccgccgcgg
      721 caacaatccg tattctcatt ttcgcgttct caagcttcct gaacgcggca cccacgagcg
      781 caccctgatg ctcggctgtg tcattgcatt gcgatatcta ggtcggcttg gggtcaatcg
      841 cgtcgttctg acggtgtgtc gatcagttcc ggtctagccc gataagccga caaatctgca
      901 cgggtagcca tcgctatcgc ttggcggccg gctccgcctg ctttgccggc ggttcggact
      961 gcaccttgca gctcgatgcg gcgagcgagc cttgcgcctg cggcatcagg ccgggctccg
     1021 gcgccagcgc ctttaatacg atcaacccgg ttgcagttgg ggaatcgatg atcaagcggt
     1081 cggccgcggt cacgtcctcg tcttccggca agtcggcgtg ttgggcttcg gtcatcagat
     1141 cgagctcgat caccttgcgg ccggcggatc ggtcgttgat ggcgtaatag gcgacttcgt
     1201 tgcgggtata gaacgacacc gaggtgtagg cctggctgac cggcaccgcc agtttgatcg
     1261 gcccagcgga cagatcgtag cagcaaatcg cgatcgcgaa cgccggatcc atgaacggca
     1321 tcagcgcgtt gttcggatcg gccaggggga gggcgatcac gctgttcagc gttgtgatcg
     1381 gggtcaggcg cgaataagcg tcctgggtcg caatgcgcgg cagtgccaga acgctgacca
     1441 gatgcacgac gccgcccagc agcgcgcccg cgatgatcgt gaacagcagg cggatcatgg
     1501 gcaccccacg gtcgcaaccg agggcatcgg cgcgtcgcgc tgcgaccgcg tcgccacacc
     1561 gaccggggta tcgtaaagcc gcagcatcag cgcgtagcgc tcgatgccgc cggtgggcag
     1621 ccagtttccg gcgcgcgatc gcgacgccac ccgtatctcg aacgcgccgt ccgccccgcg
     1681 gatgatttcc tggctggtga agccgtaacg ctgcagcgca ttcgcaacca gatgtccctt
     1741 cgaatcgtac agcgtcaacg tccagaatcg cgccgccggg gtgacgccgc tgacgaccac
     1801 atcgcagcgt ccatcgagca gtcgcttgcg gtcatccgtc gttgccgaga atgccacgcc
     1861 gtcgccggtg ccgaccggca actcgccgct gcgcgcgatc gacgcgcgtg agtacggatc
     1921 gacatcggcg gtgccggtct tcggccgggc ggtccaggca ccgatcgtca gcgtgccgag
     1981 atcggtgccg cgcgtcgccg tcacccaggt cgcgccgagg ccgacaacgg tggcaaggat
     2041 cagcgccagc agggtgataa atatcagccg cacggttagt tcttgcgcgg cacgggcata
     2101 gccgcggggg tgcctggcac ggctgccgca aagctttccg ggaatgcgac ggagctcgac
     2161 gacgccggct tgagcggctt caccggatcg ccggacgacg tttttcccgc agccctgccg
     2221 gcctcgtcga gcatcttttc cacccgcacc agaatatccg caccgcgctt ggtcaggatc
     2281 ggcggcggtc ccggcttgat atcaggaggt tttggggcac cgttagccgc aatcgtcgcg
     2341 gaaacagggg gcgcgggcaa tttcgtgccc attccaatac ccgggatctc cttgatctcg
     2401 acgccctgat gggccgcgac catgatgtcg tgccatgtct gcgccggcag cgagccgccg
     2461 gtcatccggt tggtcggcga ataatcgtcg ttgccgtacc acacggcaca ggtgaagttg
     2521 ccggtatacc cgacgaacca cgcatcgcga taggcgttgg tggtgccggt tttgccggcg
     2581 gtcggaatgc cgtcgagtgc ggcgcggcgc gcggtacctt cgctgacgac gtggctcatc
     2641 atgcccgcca tgtcggaagc gaccgatgcc ggaatggcct gaagcggctt cttgccgtcg
     2701 cggtcgaagc gccagaccag atcgccggtg ccggtgcgca cttccagcac cgaatgcggc
     2761 gtgaccgatt tacccttgtt ggggaatgtc gcataggcca cggcgtgctc gatcacggtg
     2821 acctcgtcgg cgccgatcgg cagcgagggc gtgtccggca atggcgcctt gatgccgaag
     2881 cggcgcgcca cttccacgat cttggcgcgg ccggccttcg gtccgccttt gccgccgatg
     2941 gcgatcgaca gtttgaccgg aacgacgttg atcgagcgcg tgatcgcctg ggtcagcgtc
     3001 accgcgccgg aataggagtg gccgtaattc tgcggacacc agttgccgat gcagaccgga
     3061 ccgtcgacca cgatcgacgt cggcttgaat ccgttcatca gggcggtcga gtagacatag
     3121 ggtttgaacg acgaaccggg ctgccggtag gcatccacgg cgcggttgaa ctggctggcg
     3181 ccatagtcgc gtccgccgac catcgcgcgc acgccgccgt cgagatcggc gaccacggtg
     3241 gcggcctggg tcgcgtgata gtcgcggccg aactggcgca gctggttttc gatggcttcc
     3301 tcggcggcgc gctgcacgtt catgtcgatc gcggtgcgga ccacgaagac gcgctcgaca
     3361 taggatctcg gaaaggtatc gaccagctta cgcatctcgt cgaacgccca gtcgagataa
     3421 taattcggcg aattctcgtc gcggcgatcg accgcggtgg ccggattgcg ccgggcgccg
     3481 aacacctggc cctcggtcat gaagccggcg tcgacaagat tgtcgagcac cacattggcg
     3541 cgggcgcggg cggcgggcag gttgatatgc ggcgcgtatt tggtgggcgc cttgaacagg
     3601 ccggcaagca tcgcggcttc cgcgagattg acgtcgcgcg ccgacttgtt gaaatagaaa
     3661 tgcgcggcgc cgtcgacgcc gaaggtgccg ccgcccatat aggcgcggtc gagatagagt
     3721 ttcagaattt cgttcttgga aagccgcgtc tcgagccaga ccgcgagaaa cgcctcgttc
     3781 accttgcgct cgatggtgcg ctcattgctc aggaacaggt tcttggcgag
//
LOCUS       DASWGM010000709         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1028771,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000709 DASWGM010000000
VERSION     DASWGM010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028771"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGA15_19930"
     CDS             complement(<1..500)
                     /locus_tag="VGA15_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX9590003.1"
                     /translation="MTDSVVTRFAPSPTGFLHIGGARTALFNWLYAKKHGGKMLLRIE
                     DTDRERSTEPAIAAILDGLKWLELDWDGEVIYQFTRAARHREVAEQLLAAGKAYRCYA
                     TSEELTAMREKARAEGRTRLYDGLWRDRDGSQAPSGMKPTIRLKAPQSGETVIEDQVQ
                     GRVVWQ"
     gene            643..>1676
                     /gene="glnS"
                     /locus_tag="VGA15_19935"
     CDS             643..>1676
                     /gene="glnS"
                     /locus_tag="VGA15_19935"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEX9590004.1"
                     /translation="MTAEPVAAEAGRDFIRDIVQADLDANRYSQIVTRFPPEPNGYLH
                     IGHAKSIALNFGIAQEFAGRCNLRFDDTNPTKEEQEYIDSIQADVHWLGYDWGTEVFY
                     ASDYFEQLYEWAEGLIRAGHAYVDDQSQEEIRLKRGTLTEPGQNSAFRDRPAEENLDL
                     FRRMKAGEFPNGARVLRAKIDMSSGNINLRDPVLYRILHATHPRTGDKWSIYPSYDYA
                     HGQSDAIEGVTHSICTLEFEDHRPLYEWLLDKLPVPSKPRQYEFARLNLTYTLLSKRV
                     LTELVRGGHVAGWDDPRMPTIAGLKRRGVPPAAIREFVKRIGVAKANSIVDVGMLEFC
                     IRELLNKTALR"
BASE COUNT          327 a          526 c          521 g          302 t
ORIGIN      
        1 ttctgccaga ccacgcggcc ctggacctga tcctcgatca cggtttcgcc gctttgtggc
       61 gccttcagcc ggatcgtggg cttcatgcct gacggggctt gcgacccgtc gcggtcgcgc
      121 cacaagccgt catagagccg cgtacggccc tcggcgcgcg ccttctcgcg catcgccgtg
      181 agttcctctg atgtggcgta gcagcgatag gccttgccgg cggccagcaa ttgctcggcc
      241 acttcgcggt ggcgtgccgc ccgggtgaac tggtagatga cctcgccgtc ccaatcgagt
      301 tcgagccatt tcaacccgtc caggatcgcg gcgattgccg gctcggtcga gcgctcccga
      361 tcggtgtcct cgatgcgcaa cagcatcttg ccgccgtgct tcttcgcata gagccagttg
      421 aacagcgcgg tgcgggctcc cccgatgtgg agaaagccgg tgggcgaggg ggcaaagcgc
      481 gtgacgacgg aatcagtcat tacaggagcg ggtcgggcaa agagggaaag agggcatgcg
      541 caaggcgcgg tggtgtatag caggaacctt acataactaa agccttcctt gggaggtgcg
      601 gatttggcag aaggtccgct gatccccgaa caggaactac ccatgacagc agaaccggtg
      661 gcggcagagg caggtcgcga tttcattcgt gacatcgtcc aggccgatct tgacgccaac
      721 agatacagcc agatcgtgac gcgctttccg ccggagccga acggctacct gcacatcggc
      781 catgccaagt cgattgcgct caatttcggc atcgcgcagg agtttgccgg gcgctgcaat
      841 ctgcggttcg acgataccaa tcccaccaaa gaagagcagg aatatatcga ttcgattcag
      901 gccgacgtgc actggctcgg ctacgactgg ggaaccgagg ttttttacgc atcggattat
      961 ttcgaacagc tctacgaatg ggcggaaggc ctgatccgcg ccggccatgc ctatgtcgac
     1021 gaccagtcgc aggaggaaat ccggctcaag cgcggcacgc tgaccgaacc cggccagaac
     1081 agcgcgtttc gcgaccgccc ggcggaggaa aacctcgatt tgtttcgccg catgaaggcc
     1141 ggcgagtttc ccaacggcgc gcgcgtgctg cgcgccaaaa tcgacatgtc ctccggcaac
     1201 atcaatctgc gcgaccccgt gctctaccgc atcctgcacg ccacgcatcc gcgcaccggc
     1261 gacaaatggt cgatctatcc aagctatgac tacgcgcacg gacagtccga tgcgatcgag
     1321 ggcgtcaccc attcgatctg tacgctggaa ttcgaggacc accggccgct ctatgaatgg
     1381 ctgctcgaca aattgccggt gccgtcgaag ccgcgccagt acgagttcgc gcggctcaat
     1441 ctgacctaca cgctgctgtc gaagcgggtg ctgaccgaac tcgtgcgcgg cggccatgtc
     1501 gccggctggg acgatccgcg gatgccgacg atcgccggcc tgaaacggcg cggggtgccg
     1561 ccggcggcga tccgcgaatt cgtcaagcgc atcggcgtgg caaaagccaa cagcatcgtc
     1621 gacgtcggca tgctcgagtt ttgtattcgc gaactgctca acaagaccgc gttgcg
//
LOCUS       DASWGM010000710         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1028884,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000710 DASWGM010000000
VERSION     DASWGM010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028884"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGA15_19940"
     CDS             complement(<1..185)
                     /locus_tag="VGA15_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590005.1"
                     /translation="MSVPVTDSVESARPRLFQPFEIRGLALKNRLVVPPMVHYRAGPG
                     NTCGTFHNVHLGRYALGG"
     gene            479..1402
                     /locus_tag="VGA15_19945"
     CDS             479..1402
                     /locus_tag="VGA15_19945"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9590006.1"
                     /translation="MRIAVVGAGGVGGGFGAALAKAGADVVFIARGAHLAAMKSEGLK
                     VQGGRGETHLVPTRATDDPASIGKVDIVLFCVKLWDIESAGQKIKPLIGPDTAVIPLQ
                     NGIDAAERLIPILGPDAVMGGVAQISASIVGPGLIQQVGSFMRMIFGEFDGKRRQRGE
                     DFLALCLKAGFDATSSEQILTELWMKFILLASNAGIMALARQPIGRLRDDPDLRPIFV
                     AAYREVIDVGRARGVALPADALEKILDFTGHAPPAMKPSMALDLDRGNRLELPWLGGK
                     VVELGLKLGVPTPTHSMMYAMLKPYIMGAPA"
     gene            2193..>2566
                     /locus_tag="VGA15_19950"
     CDS             2193..>2566
                     /locus_tag="VGA15_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590007.1"
                     /translation="MARELEGKVAAVTGAASGIGLASTEAMLAAGSRVVMVDRDEAAL
                     KALCNKHGDAVIPLVIDLLDPKDSATLLPRVLEKAGQLDILHANAGTYVGGDLVDADT
                     GAIDRMLNLNVNVVMKNVHDVLP"
BASE COUNT          517 a          766 c          808 g          475 t
ORIGIN      
        1 cctccgagcg cgtagcggcc gagatgcacg ttgtgaaagg tgccgcaggt gttgccgggt
       61 ccggcgcggt aatggaccat cggcggcacg accaggcggt tcttcagcgc cagcccacga
      121 atctcgaacg gctggaacaa cctgggacgt gcggattcaa cgctatcggt gaccgggact
      181 gacatcgggt tgcctgttga gtgagtggcc gacgtggggc aaagcgaata attctctatt
      241 ttgagtcgca ggtccagcag gccccttgcg agagacctcg tgggcacaca tgcgtgctga
      301 gatttctcgt catggcaatt tcggactttg cgctcgcgcc aggcgatggc cggtcgtaag
      361 gataaagcga tatgcgcctg accgggacct gcgcctgacc gggaccagag gccggacgtg
      421 ttatgctcgc tcgccgtccg gcggcggtgc caggctttcg tcagtgaggg gagattccat
      481 gcgtatcgcg gttgtcggcg ccggcggcgt tggaggcgga ttcggcgcgg cgttggccaa
      541 ggccggtgcc gacgtcgtct tcatcgcacg cggcgcccat cttgccgcca tgaagagcga
      601 gggactgaag gttcagggcg gccgcggcga gacccatctt gtgccgaccc gggcaaccga
      661 cgatcccgcc agcatcggca aggtcgacat cgtactgttt tgcgtcaagc tgtgggacat
      721 cgaaagcgcg ggccaaaaaa tcaagccgct gatcggcccc gacaccgcgg tcatccctct
      781 gcagaacggc atcgacgccg ccgagcggct gatcccgatc cttgggcccg acgccgtaat
      841 gggcggcgtg gcgcaaatca gcgcatcgat cgtcgggccc ggcctgatcc agcaggtcgg
      901 cagcttcatg cgcatgatat tcggtgagtt cgacggcaaa cgccgccagc ggggcgagga
      961 ttttctcgcg ctttgcctga aggccggctt cgacgcgacc tcgagcgagc agatcctgac
     1021 ggagctgtgg atgaagttca tcctgctcgc cagcaacgcc ggcatcatgg cgctcgcgcg
     1081 ccagccgatc ggcagactgc gggacgatcc cgacctgcgt ccgatctttg tggccgccta
     1141 ccgggaagtg atcgatgtcg gtcgcgccag gggcgtggcg ctgccggccg acgcgctgga
     1201 aaagattctc gactttaccg gccacgcgcc gccagccatg aagccgtcga tggcgctcga
     1261 tctcgatcgc ggcaaccggc ttgaactacc ctggctcggc ggcaaggtcg tggagctcgg
     1321 ccttaagctt ggcgtcccga cgccgacgca tagcatgatg tatgcgatgc tgaagcccta
     1381 catcatgggt gcgcctgcct gagcaggctt gcggtcatcg caagcaaagc gagacacttt
     1441 tgggcgatga aacaacgtcc gctgtccgag ccaaagccgg catcacttgg agctgacaag
     1501 aattcagctt ttgaccgaac cggacattaa ttccgttcag gtcgcgcccc aaccgccagc
     1561 caaacgggca tggtcttgct gacagctaat tcaaataacg caatttccgt ttgttcgcta
     1621 aggtgcgttt cgctctgttg gacgccaccg tctcaaattc ctaccaatgc cgcctacggc
     1681 gaccgtagta tccgccgccg ccaaacagca gcaccaaaac aacgacaatg aggatcaatt
     1741 ccatagcaag tctccttgga tggcaatgac ttcaaatagg gaacgacctg ccgtgaacta
     1801 agttccgcct cgcgcggagg aacgatgatg actcttcgcc cgactggcgc atgagtccgt
     1861 tgctggcacc tttgagagag acatgccgac cgactctcaa aatgtctgct tatcggggaa
     1921 gaccggaagt gaccggccaa ctgtcaaatc gacgcgaata accccaaacg gaccccgctc
     1981 tgtccagttc aggtgcaaaa ctcaagatat ttgaaacacc gcttttgacc tcgaaaaaat
     2041 gggtgctttt gagagacggc ggcgcaaccc gagcaggatg cttgcggcgg ggaacgccgc
     2101 caagcgccag gccgtccttt gtccccgaca accccacttg ggattgctat actgcggcgc
     2161 gtgttgtggt gaccccagca aggtggagac ctatggcgag agaattggaa ggcaaggttg
     2221 ccgccgtgac cggggctgcg tcgggcatcg gattggctag taccgaggcg atgttggccg
     2281 cgggctcgcg cgtggtgatg gtcgaccgcg acgaggcagc gctgaaggcg ctctgcaaca
     2341 agcacggcga cgcagtgatc ccgctggtca tcgacctgct cgacccaaag gactccgcta
     2401 cgctgctgcc gagagtccta gagaaggcag gccaactcga catcttgcat gcaaatgcgg
     2461 gtacgtacgt cggcggcgac ttggtcgatg ccgacactgg ggctatcgac cggatgctga
     2521 acttgaatgt caacgtcgtg atgaagaatg tgcacgacgt gctgcc
//
LOCUS       DASWGM010000711         5226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1029593,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000711 DASWGM010000000
VERSION     DASWGM010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029593"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VGA15_19955"
     CDS             <1..1125
                     /locus_tag="VGA15_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9590008.1"
                     /translation="LTLRSTQLASSTFDFFSIFFASGLIYLALTTGVSVIQLLVERVL
                     DLDRPPAQFRLARLLPRYLAPLALEPITAEPIAPTCAGSGETLPDAAPVKDAKASRAD
                     RTRRSATLTRNGAVVEVSGLYKAYDSQAVLAAVDMTVRDGEVVALLGPSGSGKSTLLR
                     CINHLEGWDAGTVKVGGRRMGFREDGKPLSPRDLANERAMVGVGMVFQQFNLFGHLTA
                     LENVAGPLRWVHGVSRADADRRARELLERVGLSHRADALPRHLSGGQQQRVAIARAIA
                     PNPSVLLLDEPTSALDPELVNEVLEVIRRLTVEDGLTMIISTHQLRFADEVADRVVFL
                     SGGSIIEEGPAHEVLTSPRNPVTARFLSIMEADRPTAAPA"
     gene            1215..2456
                     /locus_tag="VGA15_19960"
     CDS             1215..2456
                     /locus_tag="VGA15_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX9590009.1"
                     /translation="MKHHLLPVSPKNVQWGYFSKKIAPALTLKSGDRATIETLTHHAN
                     DDHERMIAGDPGAESVFHWTREHKAIVRRGAGPTEGPFVRGSGEGVGVHLLTGPVAIE
                     NAEPGDILEVRILDVRPRPSCNACHAGRCFGSNAAASWGFHYRDLIEEPKPREVITIF
                     ELDTSGEPFARAVYNYVWTPQTDPDGIVHSTIDYPGVRVDHTLVRKRENILPNVKVPA
                     RLHFGTMGLAPAESDFVSSIPPGYTGGNIDDWRIGKGARMYYPVAVSGAFFSVGDPHA
                     AQGDSELGGTAIETSLTGDFEFILHKKGDLGGTVLEALTHPMLETEQQWSMYGFTFSN
                     YLAELGPNAQTDVANHSSLDKAMRDAFRKLRRFLMTVHRMSEDEAIALMSVAADFGVT
                     QVVDANWGVHGSIRKNVFMCT"
     gene            2596..4935
                     /locus_tag="VGA15_19965"
     CDS             2596..4935
                     /locus_tag="VGA15_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX9590010.1"
                     /translation="MSIHPFTSESYSEGDRTEAWQDVLGGFGLRSQPMSAIHGEHAMA
                     LSRASLDGVGLMRFAAAPQVFSPLPRRADLPILLLPTEDGAVLKSNGSQQGVAAGRII
                     LLPRQGDWQVAFHRNIRAIVMSVTVESFGGRRISLPEYRDAGIVPSGGLADVLRGTLE
                     ATSEALETLSPEAWNTIRLSLAEMLLTLARQKSRSATEMVGIGAQPALLDRIYEAIER
                     KLGDPDITPARVAQIEGISERYLQKLFEGTGDNFTHYLRERRLQRCSADLANPAEAHR
                     SVSDIAFGCGFSDAAHFSRSFRDRFGMSPRAFRQQQAQRASGLGVRTGQRGWPQDALA
                     QLRPTRAAPVIAPAQLKLVDFAVPRGSKPAHHFLPVDATRVHWGFFSRALKPLIEIVS
                     GDTVTVETLTQHASDDPELMISGDPGAESVFRWTRETKGVERRGAGPMDASIYGRGAG
                     EGFGVHICTGPIAVKGAEPGDVLEVRILDMVPRPSRNPDLEGRIFGSSVAAWWGYHYD
                     EFLSEPKPREAVTIYEIFPGDEPYAQALYSYRWQPQKDPYGVVHQTYDYPGIPVAADS
                     IRRRHGVLDGVRIPLRPHFGVIGVAPREAGLVDSVPPAYFGGNLDNWRLGKGAAVYLP
                     VSVPEALLSIGDPHAAQGDGELSGTAIECSMTGTFQIVLHKKADCAGQPFADLTYPLI
                     ETETDWVLTGFSHPNYLAEFGAKGQSEVYAKSSLDLAMKDAFRKVRRFLVNTKGLSED
                     EAIALISAAVDFGVTQVVDGNWGVHAILSKRLFAHEDEL"
     gene            complement(5065..>5226)
                     /gene="ssrA"
                     /locus_tag="VGA15_19970"
     tmRNA           complement(5065..>5226)
                     /gene="ssrA"
                     /locus_tag="VGA15_19970"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
BASE COUNT          944 a         1665 c         1625 g          992 t
ORIGIN      
        1 ctgacgctgc gaagcacgca gctcgcatcc tcgaccttcg acttcttttc gatcttcttt
       61 gcatccgggc tgatctatct ggcgctcacc accggggtca gcgtcatcca gttgctggtg
      121 gaacgtgttc tcgatctcga ccggcccccg gcccagtttc gtctcgcgcg cttgctgccc
      181 cggtatctcg cgccgttggc gctggagccg atcaccgcgg agcccatcgc tcctacctgc
      241 gcaggatccg gcgaaaccct ccccgacgcc gcgcctgtca aggacgccaa agcgtcgagg
      301 gcggaccgaa ccaggcggtc ggcaaccctg acgcgcaacg gcgcggtggt cgaggtcagc
      361 ggattataca aggcctatga cagccaggcg gttctcgccg ccgtcgatat gacggtgcgc
      421 gatggcgaag tcgtagcatt gctcggcccg agcgggtcgg gcaagagcac gctgctcaga
      481 tgtatcaacc atctcgaggg ctgggatgcc ggaaccgtca aggtcggcgg ccgccgcatg
      541 ggttttcggg aagacggcaa accgctttcg ccgcgtgacc ttgccaatga gcgcgccatg
      601 gtcggcgtcg gcatggtgtt ccagcaattc aatctgttcg gccatctgac ggcgctggag
      661 aatgtcgcgg gtccgttgcg ctgggtccac ggcgtttcgc gggccgacgc cgatcggcgc
      721 gcgcgagaac tgctcgaacg cgtcggcctc agccaccgcg ccgacgcgct gccgcggcat
      781 ttgtccggtg gccagcagca gcgcgttgcg atcgcgcgcg cgattgcccc gaacccgagc
      841 gtgctgctgc tcgatgagcc gacctcggcg ctcgatccgg aactggtcaa tgaagtgctg
      901 gaggtgatcc ggcgcctcac ggtcgaggat ggcctgacca tgatcatctc gacccaccag
      961 ctgcggttcg ccgacgaagt cgccgaccgc gtggtctttc tcagcggcgg ttcgattatc
     1021 gaagagggac ctgcgcacga ggtccttacc agcccgcgca atccggtcac ggcgcgattt
     1081 ctcagcatca tggaggccga cagaccaacc gcggcgcccg catgaggcag ccttgcgccg
     1141 ccttctcaac ctagggtgac atccttcaac tcaaaaccaa cttacgtccg caagcgttct
     1201 ttcgaggcca ccgcatgaaa catcatctcc ttcccgtttc tcccaagaac gtccagtggg
     1261 gctatttcag caagaagatc gcgccggccc tgaccctgaa gtccggcgac cgcgccacca
     1321 tcgaaacgct gacccatcac gccaacgacg accacgagcg gatgatcgcg ggcgatcccg
     1381 gcgccgaaag cgtgtttcat tggaccaggg aacataaggc tatcgtccgc cgtggcgcgg
     1441 ggccgaccga aggtccgttt gtgcgcggct ccggcgaagg cgttggcgtc catctgctga
     1501 ccggtcccgt tgcaatcgag aacgccgagc ccggcgatat cctggaagtt cgcatcctcg
     1561 acgtccggcc gcgtccgagc tgcaatgcct gccatgccgg gcgctgcttc ggttcgaacg
     1621 ccgccgcgtc atggggattt cactatcgcg atctgatcga ggagccgaag ccgcgcgaag
     1681 tcatcacgat cttcgagctc gacacgtcag gtgagccatt cgccagagcg gtctataact
     1741 atgtctggac accgcagacg gatcccgacg gcattgtcca ttcaaccatc gattatcccg
     1801 gcgtgcgcgt cgatcacacc ctggtgcgaa agcgcgaaaa catcctgccg aacgtcaagg
     1861 taccggcgcg tctccacttc gggaccatgg ggctagcgcc cgcggaatcc gacttcgtga
     1921 gttcgattcc gccgggctac accggcggca atatcgacga ctggcgcatc ggcaagggcg
     1981 cgcggatgta ttatccggtc gcggtatcgg gcgcgttttt ctcggtcggc gatccgcacg
     2041 cggcccaggg cgacagcgag ctcggcggga ccgcgatcga aacctcgctg accggcgact
     2101 tcgaattcat cctgcacaag aagggcgatc ttggcggcac ggtgctggag gcgctcaccc
     2161 atccgatgct ggaaaccgag cagcaatggt cgatgtacgg ctttacgttt tcgaactacc
     2221 tcgccgaact cggtccaaac gcccagaccg atgtggcgaa ccattcgagc ctcgacaagg
     2281 cgatgcgcga tgcctttcga aaacttcgcc gctttctcat gaccgtccac cgcatgagtg
     2341 aggatgaagc gatcgccctg atgtcggtcg cggccgattt cggcgtcacc caggttgtcg
     2401 atgccaactg gggcgttcac ggatccatcc ggaagaatgt ttttatgtgc acgtgaaaaa
     2461 accgccggtc ccggctgctg acgcgcagtc ccgacgctga attgccataa cggcggcaaa
     2521 accgccgatc gccgtggcgt gcctgttgtt ggcacgagcg ttgcttcggt ttggcatgtc
     2581 agcgttggcc agaccatgag tattcacccg tttaccagcg aatcctactc cgaaggagac
     2641 aggaccgaag cctggcagga cgtgttgggc ggattcggcc tgcgatctca gccaatgtcg
     2701 gccatccatg gcgagcatgc catggcgttg tcccgggcat ctttggacgg cgtcgggctg
     2761 atgcgttttg ccgcggcccc gcaggtattt tcgccgctgc cgcggcgcgc cgatctcccg
     2821 atcctgctgc tgccgaccga agacggcgcg gtgctcaagt caaacggatc gcaacaaggc
     2881 gttgcggccg gccgcatcat cctgctgccc cgccagggcg actggcaggt cgcgtttcac
     2941 cgcaacatcc gcgccatcgt catgtcggtg acggtcgaga gttttggggg gcgcaggatc
     3001 agtctgcccg aataccggga cgccggcatc gtgccgtcgg gaggattggc cgacgttctt
     3061 cgcggcacgc ttgaggctac gtccgaagcc ctcgaaaccc tgtcgccgga agcgtggaac
     3121 acgatccggc tcagtcttgc cgagatgttg ctcacccttg cgcgccagaa gtcgcgcagc
     3181 gccaccgaaa tggtgggcat tggcgcgcaa ccggcgttac tcgatcgcat ttacgaggcg
     3241 atcgagcgca agctcggcga tcccgatatc acgccggcgc gggtcgcgca gatagaaggt
     3301 atctcggagc gctatcttca gaagctgttc gaaggcaccg gcgacaattt cacgcattac
     3361 ttgcgtgagc gccggctgca acgctgctcg gccgatctgg caaatccggc cgaggcgcat
     3421 cgttcggtgt ccgacattgc ctttggttgc ggattcagcg acgccgccca tttcagccgc
     3481 tcgtttcgcg accggttcgg catgtcgccg cgggcgttcc ggcaacagca ggcccagcgc
     3541 gcttcaggtc tgggcgtccg gaccgggcag cgcggctggc ctcaggatgc gctggcgcaa
     3601 ttgcgcccca cacgggcggc gccggtgatc gcgcccgcgc aactgaagct ggtggacttc
     3661 gcggtcccgc gcgggtccaa gcctgcgcat cattttctcc ccgtcgatgc gacgcgggtt
     3721 cactggggct ttttcagccg cgcgctaaaa ccgctgatcg aaattgtgtc gggcgacacc
     3781 gtcacggtcg agaccctgac gcagcacgcc tccgacgatc ccgagctgat gatatcaggc
     3841 gatcccggcg ccgaaagcgt gtttcgctgg acgcgcgaga caaagggtgt cgagcgccgc
     3901 ggcgccgggc ctatggacgc gtcgatttac ggtcgcggcg ccggcgaagg ctttggggtc
     3961 cacatctgca ccgggccgat tgcggtaaag ggcgctgaac ccggcgacgt gctggaagtc
     4021 cgcattctcg acatggtgcc gcgcccgagc cgcaatccgg atctggaggg ccggattttc
     4081 ggcagcagcg tcgccgcctg gtggggatac cactatgacg agtttctttc ggagcccaag
     4141 ccgcgcgaag ccgtcacgat ctatgagatt ttccccggcg acgagcccta tgcgcaggcg
     4201 ctctattcct atcggtggca gccgcaaaaa gatccatacg gcgtcgtgca ccagacctac
     4261 gactatcccg gaatacccgt cgcagccgat agcatcaggc gacggcatgg cgtgctcgac
     4321 ggcgtccgca ttccgctgcg gccgcatttt ggcgtcatcg gcgtggcgcc gcgtgaggcc
     4381 ggcctggtcg attccgtgcc gccggcttac ttcggcggca atctggataa ttggcggctc
     4441 ggcaagggcg ccgcggtcta tcttccggtt tcggttcccg aagcgctgtt gtcgatcggc
     4501 gatccgcatg ccgcccaggg cgacggcgag ttgagcggaa cggcgatcga atgttcgatg
     4561 accgggacat ttcagatcgt gctgcacaag aaagccgact gtgccggcca accgtttgcc
     4621 gacctgacct atccgctgat cgaaaccgag accgattggg tgctgaccgg attcagccat
     4681 ccgaattatc tcgcagaatt cggcgccaag ggacagagcg aggtctacgc caaatcatcg
     4741 ctcgatctgg cgatgaagga tgcgtttcgc aaagtgcgcc gctttctggt gaataccaaa
     4801 ggcctgagcg aagacgaagc catcgcgctg atctcggcgg cggtggattt cggcgtcacc
     4861 caagtggtcg acggcaattg gggcgtccac gccatcctga gcaagcgcct gttcgcgcat
     4921 gaagacgagc tgtgatgaca ggggattatg cgcttcttcg cgctcctatt gtaattgagt
     4981 ttccaataaa tcggtttcaa cgaattactg catcggccaa accaagccca caaaataaag
     5041 ccgcgccaac ccgagggttg acgcggcgat ggccgttctg ttgccaggtg gacctacccc
     5101 gaacaggtta cgccgcgagg cgcacttcgt tcaatgcaac gttatcgttt gcatttaggt
     5161 ttttgacccg ataacggcgg tatcatgccg agcacaattc agagcttctt cgcgaccatc
     5221 gatcct
//
LOCUS       DASWGM010000712         3143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1445253,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000712 DASWGM010000000
VERSION     DASWGM010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445253"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..396
                     /gene="hspQ"
                     /locus_tag="VGA15_19975"
     CDS             64..396
                     /gene="hspQ"
                     /locus_tag="VGA15_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEX9590011.1"
                     /translation="MIKARTAKFQIGQIVRHRIFSFRGVIFDIDPVFNNTEEWWLSIP
                     EEMRPHKDQPFYHLLAENSESEYVAYVSEQNLLPDDSGEPIRHSQVAEIFVKDKSGGY
                     RPRNPSLN"
     gene            complement(627..1448)
                     /locus_tag="VGA15_19980"
     CDS             complement(627..1448)
                     /locus_tag="VGA15_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX9590012.1"
                     /translation="MIFRILAAPVWPRGRVFALLAATALAAAFLAPDVQAQAPAPGVP
                     AAPKAKAAAPKAAPKAAPAAPAPTAQQQAPPAAGAPAAGAQPPDQQVQLIYAPWTKFC
                     LKGQDANAKQVCFTGKDGRIESGQPVIAAVIIEPEGEPKKILRVTLPLGMQLVHGTRV
                     IVDGNPPAQSPYVICFANGCMSDYDVTPELLANMKKGQNLVVQAINSNGAPLTLPLPL
                     AEFAKAYDGPPTDPKVFEETQKKLQEELQKRAEEARKRLEANPPNGAAAANPAAK"
     gene            1812..>3143
                     /locus_tag="VGA15_19985"
     CDS             1812..>3143
                     /locus_tag="VGA15_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9590013.1"
                     /translation="MHGAAALPADFTHLPYANPDAPKGGRLVQGVLGTFDSLNPLIVR
                     GVAVQQVAAVQPMRGLVIESLMARGNDEAFTLYGLLARSVETDDTRSYVTFHIDPRAR
                     FSDGQPVTAEDVLFSWALLRDKGRPNHRQYYSKVAKAEAPDPLTVRFDLSANDRELPL
                     ILGLMPILPKHAVDVATFEETTMTPPIGSGPYRVSAVKPGASVTLTRNPDYWGLNLPV
                     NRGLWNFDEIRLDFYREANGQFEAFKRGLYDFRVENEPLRWQNGYDFPAARNGEVIRD
                     TIKTGMPQPSEFLVFNTRRPMFSDIRVRQALTLLFDFEWINRNYFFGLYTRAAGFFAG
                     SELSAYGRAAGDRERELLKPFVSHIPPDILDGSYRLPVSDGSGRDRATLRSALKLLSE
                     AGYDLDGTVLRQRSTKAPLTFEILVTTRDQERIALAYTRDLKRAGIEATVRA"
BASE COUNT          576 a          982 c          961 g          624 t
ORIGIN      
        1 gcaagggtta attcgtccgt ttgcctctag catcggccac aatcatggtc tattcgacga
       61 gatatgataa aagcccggac cgccaaattt cagatcggac agatcgtccg tcaccggatc
      121 ttttcgttcc ggggcgtgat cttcgatatc gatccggtct tcaacaatac cgaggaatgg
      181 tggttgtcga tccccgagga gatgcggcct cacaaggatc agccgttcta tcatctgctc
      241 gccgagaatt cggaatccga atacgtcgcc tacgtctccg aacaaaatct gttgcctgac
      301 gattccggcg agccgatccg gcactcgcaa gtcgccgaga ttttcgtcaa ggacaaatcg
      361 ggcggctatc gcccacgcaa cccatcgctg aattgagatc tgtcgcctgg aggaaccgtc
      421 atcctgagga gcggcctctt ggccgcgtct cgaaggatgg ccacgcgccc tttcgcttgt
      481 catccttcga ggctcgcaag ggctcgcacc tcccgagtga gcgcaaatgc gctcatccgg
      541 ggatgacgtt gaaccctccg cctcaataaa aaaggcgccc gatccgagcg cctttttggt
      601 ccgttgataa agaagggttc tgcttgctac ttcgcagccg gattggccgc agccgcgcca
      661 ttgggcggat tagcctcgag ccgcttgcgc gcttcctcgg cgcgtttctg caactcttcc
      721 tgcagcttct tctgggtctc ttcgaacacc ttcgggtcgg tcggcgggcc atcataggcc
      781 ttggcgaatt ccgcgagcgg cagcggcagg gtgagcggcg cgccgttgga gttgatcgcc
      841 tgaaccacca agttctgccc cttcttcatg ttcgcgagca gttccggggt gacatcgtag
      901 tccgacatgc agccgttggc gaagcagatc acataggggc tctgcgccgg cggattgccg
      961 tcgacaatca cgcgggttcc gtgaaccagt tgcatgccca gcggaagcgt gacgcgcagg
     1021 atcttcttcg gctcgccttc cggttcgatg atcacggctg caatcaccgg ttggccggac
     1081 tcgatgcggc catccttgcc ggtgaagcag acctgcttgg cattggcgtc ctggcccttc
     1141 aggcagaact tggtccaggg cgcatagatc agctgaacct gctggtccgg gggctgggca
     1201 ccggccgcgg gagcgccagc cgccggcggg gcctgctgct gagcggtcgg tgcaggtgcc
     1261 gcaggagccg cctttggcgc ggctttcggc gccgcggcct tggccttcgg cgcagccgga
     1321 acaccgggtg caggcgcctg ggcttgcacg tctggagcta ggaacgcggc ggccagtgcc
     1381 gtcgccgcca acagcgcgaa aacccgccca cgcggccaga ccggcgcggc caagatacgg
     1441 aaaatcattg cggaaaaccc tttctgaacg gaagcgcccg aaaccgctgc aggcaccgac
     1501 accttgccgt ttgggtccgg ctgtctcctt gccaattgag gcggatacgt gacagggccg
     1561 gccgtcttgg tcaatggcac gtcttcatac agtggcagac gaaaagatca atggcgacgg
     1621 gcatcattgc cacgcgccgg gaccggatat gacggctgtg ataagactgg actgtgacta
     1681 atatcgaatc aaatcagcac gtgtcattgc gagtttccag gtggcggcgc cttcgaaccg
     1741 tggcctgtgc gatcatcggt atctggctcg cgctggatcc ctgccatgca ggcgacaact
     1801 acgccatcgc catgcatggc gcggcggcct tgccggccga tttcacccat ctgccctatg
     1861 ccaatcccga tgcgccgaag ggtggccgac tggtgcaggg cgtcctcggt accttcgaca
     1921 gcctcaaccc cctgatcgtc aggggagttg ccgtgcagca ggttgccgcc gtgcagccaa
     1981 tgagaggcct cgtgatcgag agcctgatgg cgcgaggcaa tgacgaggcc ttcacgctct
     2041 atggtctgtt ggccaggagc gtggagaccg acgacaccag gagttacgtg accttccata
     2101 tcgatccgcg ggcgcgcttc tcggacggcc aaccggtaac ggccgaggat gtgctgtttt
     2161 catgggcgtt gctgcgcgac aagggccgtc caaaccatcg ccagtactat tccaaggtag
     2221 ccaaggccga agcgcccgac ccgctcacgg tacggttcga cctcagtgcc aatgaccgcg
     2281 aactgccatt gatcctcggg ctgatgccga tactgcccaa gcacgccgtc gatgtcgcga
     2341 cgttcgaaga gaccacgatg acgcccccga tcggttccgg cccctaccgc gtcagcgcgg
     2401 tcaagcccgg cgccagcgtg acgctgacgc gcaatcccga ctattggggc cttaacctgc
     2461 cggtcaatcg agggctatgg aatttcgacg agatcaggct cgatttctat cgggaggcca
     2521 acggccagtt cgaggcgttc aagcgcggcc tctatgactt tcgcgtcgag aacgaaccgc
     2581 tgcgctggca aaacggatac gactttccgg cggcccgcaa tggcgaggtg atccgcgaca
     2641 ccatcaagac cgggatgccg caaccttcgg aattcctggt gttcaacaca cggcgtccga
     2701 tgttttccga tatccgcgtg cgccaggcct tgacgctgct gttcgatttc gaatggatca
     2761 accgcaatta tttcttcggg ctctatacgc gcgcggccgg cttctttgcg gggtctgaat
     2821 tatcggcgta tggccgcgct gctggcgacc gcgagcggga gttgttgaag ccatttgttt
     2881 cgcacatacc ccccgatatt ctcgacggca gctaccgcct tcccgtcagc gacggctctg
     2941 gccgcgatcg cgcaacgctc cgcagcgctc tgaagctgtt gtcggaggct ggctacgatc
     3001 ttgacgggac ggtattgcgg cagcgctcga ccaaagcccc cctcaccttc gaaattctgg
     3061 tgacgacccg cgatcaggag cggatcgcgc tggcgtatac gcgcgatctg aagcgggcgg
     3121 gcatcgaggc cacggtgcgc gcg
//
LOCUS       DASWGM010000713         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2690320,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000713 DASWGM010000000
VERSION     DASWGM010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2690320"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VGA15_19990"
     CDS             complement(<1..249)
                     /locus_tag="VGA15_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9590014.1"
                     /translation="MSTKAPNPVDKYVGSRVRMRRIMLGMSQEKLGEALGLTFQQVQK
                     YEKGTNRVGASRIQQISEILQVPVSFLFEGGPSGTARAD"
     gene            complement(325..1932)
                     /gene="lnt"
                     /locus_tag="VGA15_19995"
     CDS             complement(325..1932)
                     /gene="lnt"
                     /locus_tag="VGA15_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9590015.1"
                     /translation="MDKLRAAGLAIILSWGWKRAAIALVAGALSSLAMAPFNAWPVLF
                     LTFPVMVWLIDGAGGGRLHGLPAAAMAGWWFGLGYFVPGLYWIGYAFLVDAPTFAWLM
                     PFAVLGLPAYLALFPAFGFALARLIWSKDGSRVIALAASLTASEWLRGHVLTGFPWNA
                     FGYALTKPLALAQAASLIGLWGLTFLSVAIFASPAVLIDGNSRGRRPWVAPVMALLVL
                     VAIGIFGAVRLSLQPTTTVANVKLRIMQPNLQQDVRFNYAAKAEVMQKYLTLSDRASG
                     PQSTGVRDASILIWPESAFPFFLAREADAMAQIDDLLPKGTILITGSVRAPDLPPGTK
                     ITRAYNSIYAIDHDGSVLAIYDKLHLVPFGEFLPFQDWMEKLGFVQLTKVQGGFIPGT
                     GRRAMEIPNAPRALPLICYEAIFPDEVAARGDRPGWIINLTNDGWFGISTGPYQHLQQ
                     ARLRAIEQGLPVVRAANTGISAVIDPMGRIVARLGLGVEGVLDSSLPAAIAPTIYARS
                     GDIPTAMMVAAAFILVIRRRVAKRSRL"
     gene            complement(1938..>2750)
                     /locus_tag="VGA15_20000"
     CDS             complement(1938..>2750)
                     /locus_tag="VGA15_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX9590016.1"
                     /translation="DIPLGELMSLFESAAHSRLVVYNETLDDPEGIVHIRDLLAFMTA
                     KARVSDSSKAKRKKPFPAGLDLRAVDLALPLSEANIIRKLLFVPPSMRAIDLLAQMQA
                     SRIHLALVVDEYGGTDGLVSIEDIVEQIVGEIDDEHDSDEPPAIMRQADNSFIADARA
                     SLDDVRSVIGEDFVTGEAGEEVETLGGYLVTHVGRLPVRGEVISGPGNFEIEVLDADP
                     RRVKRLRIAPRKERPPARTQRESRRREAPPEPGPAQANDNASPPTGDGAGSQ"
BASE COUNT          531 a          872 c          870 g          477 t
ORIGIN      
        1 gtcggcgcgc gcggtgccgc tgggtcctcc ctcgaacaag aacgataccg gcacctgaag
       61 tatttcggag atctgctgga tgcggcttgc tccgacccgg ttggtgcctt tctcgtactt
      121 ctgaacctgc tggaaggtga gacccaatgc ttcgccaagc ttctcctggc tcatgccgag
      181 catgatccgg cgcatgcgca cacgactgcc gacatatttg tcaacagggt tgggcgcttt
      241 ggtcgacatc tccaacactc cttgggatta cgctggtggg gtgaggcaaa acgaaaacgg
      301 agtatgcctc aggcgctggt accgtcaaag cctgcttcgt ttggcgacgc gccggcgaat
      361 gacgagaatg aaggcggcgg cgaccatcat ggctgtgggg atgtcgccgc tgcgcgcgta
      421 aatggtcggc gcaattgcag ccggcaggct cgaatccaat acaccttcga cgccgaggcc
      481 gagccgtgcg acgatccgtc ccatcggatc gatcacggcg gaaatgcccg tgttcgccgc
      541 acgaaccacc ggcagtcctt gctcgatcgc gcgcagccgc gcctgttgca agtgctggta
      601 ggggccggtt gagattccaa accagccgtc attggtcagg ttgatgatcc agccggggcg
      661 atcgccgcgc gccgcaactt catcgggaaa gatcgcctca taacagatca agggtagcgc
      721 gcggggagcg tttggtatct ccatcgcgcg gcgccccgtg cccggaatga atccgccctg
      781 aaccttggtc agctgcacaa agccgagctt ttccatccag tcctggaacg gcagaaattc
      841 gccgaacggc accaggtgca gcttgtcgta aatcgccagc acgctgccgt catggtcgat
      901 ggcgtaaatc gaattgtagg cgcgcgtgat cttggtgccc ggcggcaaat cgggcgcgcg
      961 caccgagccg gtgatcagaa tggtgccctt gggcaacaga tcgtcgatct gcgccatcgc
     1021 atcggcttcg cgcgccagga aaaacgggaa cgccgattcc ggccagatca ggatgctggc
     1081 gtcgcgcacg ccagtggatt gcggcccgga cgcgcgatcg gagagcgtca gatatttttg
     1141 catcacctcc gccttggcgg cgtaattgaa cctgacgtcc tgctgcagat tgggctgcat
     1201 gatccgcaat tttacattcg caacggtcgt ggtcggctgc agacttaagc gaaccgcccc
     1261 gaaaatgcca atggcgacga gcactagcag cgccatcacc ggcgcgaccc aaggtcttcg
     1321 gccgcgtgaa ttaccgtcga tcaacacggc cgggctggca aaaatcgcga cgctcagaaa
     1381 tgtcaggccc cacaggccga tcagcgacgc cgcctgcgcc agcgccagcg gtttggtcag
     1441 cgcgtagccg aacgcattcc atggaaagcc ggtgaggaca tgaccgcgca gccattcgct
     1501 tgccgtcagg cttgcggcga gcgcgatcac ccgcgaacca tccttgctcc agatcaggcg
     1561 cgcaagggcg aagccgaacg cggggaacag cgccagataa gcgggcaggc ccagcacggc
     1621 gaacggcatc agccatgcga atgtcggtgc atcgaccagg aacgcgtagc cgatccagta
     1681 gagtcccggc acgaaataac caaggccaaa ccaccagccg gccatcgccg ccgcgggcag
     1741 gccgtgcagg cgtccgccgc ccgcgccgtc gatcagccag accatcaccg gaaaagtcag
     1801 gaacagcacg ggccatgcat tgaacggcgc catcgcgagc gacgacagcg cccccgcgac
     1861 gagcgcaatg gcggcgcgct tccagcccca tgacaggatg atcgcaagac cggccgcgcg
     1921 aagtttgtcc atgggtttca ctgcgagcca gctccatcgc cggttggtgg gctcgcatta
     1981 tcattggcct gtgcgggacc cggctccggc ggggcttcgc ggcggcggct ctcgcgctgc
     2041 gtgcgcgctg gcgggcgttc cttgcgcggt gcgatgcgca ggcgcttgac gcgcctcgga
     2101 tcggcgtcga gcacttcgat ttcgaaattg ccggggcctg aaatgacttc gccacgcacc
     2161 gggagacggc cgacatgggt cacgagatag ccgccaagcg tctcgacttc ctctccggct
     2221 tcgccggtga cgaaatcctc gccgatcacg gagcggacgt catcgaggct ggcgcgcgcg
     2281 tcggcgatga aggaattgtc cgcctgccgc atgatcgccg gcggctcgtc gctgtcgtgc
     2341 tcgtcgtcga tctcgccgac gatctgttcg acgatgtcct cgatcgacac caggccatcg
     2401 gtgccgccat actcatccac caccagcgcc aaatggatgc gcgaggcctg catctgcgcc
     2461 agcagatcga tcgcgcgcat cgatggcggc acgaaaagca gtttccggat aatgttggcc
     2521 tccgagagcg gcagcgcgag atcgaccgca cgcagatcga gacccgcggg gaacggcttc
     2581 ttgcgcttgg ccttgcttga atccgataca cgcgctttcg cggtcatgaa cgccaggaga
     2641 tcgcggatgt ggacgatgcc ttcgggatcg tcgagggttt cgttgtagac caccaggcgc
     2701 gaatgcgccg cgctttcgaa caggctcatc aattcgccga gcggaatgtc
//
LOCUS       DASWGM010000714        10464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_617548,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000714 DASWGM010000000
VERSION     DASWGM010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617548"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VGA15_20005"
     CDS             <1..602
                     /locus_tag="VGA15_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590017.1"
                     /translation="KRSSAVRLRSRPLISIRCGSLRFRFSVGHEKQSRRYHTSFNVQS
                     CAHLVRQKYFSIVDRRPESNRPGAGRGEQDKAGVPRRRGKIINIASVQSELARPGIAP
                     YTATKGAVKNLTRCMCADWAQYGLQINAIAPGYFNTPLNQALVDNPEFSCGLEKRTPA
                     ARWGNVAELVGAAVFLSGRASSFVNGHTLCVDGGITTCL"
     gene            685..1716
                     /locus_tag="VGA15_20010"
     CDS             685..1716
                     /locus_tag="VGA15_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008529987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HEX9590018.1"
                     /translation="MKAVVIHAARDLRVEEREPEAAGAGQVEVAVEAGGICGSDLHYF
                     NHGGFGTVRVREPMILGHEVAGTIKALGAAVSGLAIGDRVAISPSRPCNICNYCLKGQ
                     QNQCLNMRFYGSAMPMPHIQGAFRQRLVAEAWQCHKVADGVSINEAAFAEPFAVVLHA
                     VSRAGSLLGKRVLVTGCGPIGMLAIMAARLHGAREIVATDVVAAVLEKALRLGADRTI
                     NVADDPHQLGTYSANKGYFDVQFEASGNERAVRSGLEVLKPRGILVQLGLGGDVSIPQ
                     NMVVAKEIEMRGTFRFHEEFGLAVDLINQRRVDLTPLLTGVFGLDEAIHAFEIAGDRS
                     RSMKVQLRF"
     gene            complement(1731..2510)
                     /locus_tag="VGA15_20015"
     CDS             complement(1731..2510)
                     /locus_tag="VGA15_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590019.1"
                     /translation="MDLGIEGRTALVCASSQGLGLACAQALAQEGAHVVINGRDTAKL
                     DRTAAELREAARGDVIAVAADVTTTDGQARLLAACPSPDILVNNNAGPSPGNFADIER
                     DRWLATIEANMIAPLMLIRAVLPAMRQRRFGRIVNITSAMVTTPRPHMTLSSGARGGL
                     TAAMKGLSLEVARDNVTINNLLPERFDTDRQHQMAKAAMAREDISYEEARARQIESIA
                     ARRLGDPKEFGATCAFLCSGLAGFMSGQNLHLDGGSYPALI"
     gene            2704..3708
                     /gene="dctP"
                     /locus_tag="VGA15_20020"
     CDS             2704..3708
                     /gene="dctP"
                     /locus_tag="VGA15_20020"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9590020.1"
                     /translation="MTRFCMALLAWFAVFTPLTAGAEPVTLKLSFFASETEVNYARAI
                     KPWIDAVNADPSGAVKIEAYPNGALGKALPAQPQLLLDGVADIAFVNPSLGPGRFPDD
                     QVFEVPGLLQNLKEGVKLYEELLKANLLRGYSDYYVVGAFMNPQYMVFARRPIKSLAD
                     LKGMKVRINGAVIGQTVKELGMVPVLMPPNEVVEAMGRGTIDATTTVPAAIVDFGIDR
                     VTSHDYLLPLGAGPLAVLMNRKKFESLPKPAQDVIAKYSIRWIDDTYIQDLGQYNDEL
                     IARFKADPKRVVTSPSQADIASLQPIYLKVTAEWVAKSPGNAELLGKARELLAGIRAN
                     "
     gene            3710..4219
                     /locus_tag="VGA15_20025"
     CDS             3710..4219
                     /locus_tag="VGA15_20025"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9590021.1"
                     /translation="MKAARTIVTGIANAFAVAGLSLLLILAVFTLLDGLLRAVANTPI
                     DLVREIGDMVAAICGTCCLPIVLLHQKNIVLRIFDRILPPSGVRVIDTIAALAIEIVM
                     IGMAWQFWLFGIKTMRAGDVTWLLNWPKAPFWFAVDGILWAAVVVQAFVLVEEMTGAG
                     RQRGPETVA"
     gene            4216..5520
                     /locus_tag="VGA15_20030"
     CDS             4216..5520
                     /locus_tag="VGA15_20030"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9590022.1"
                     /translation="MTATGIGIGGIVALFVLVALHIPLAFAMIAVGIVAFALQTDWSS
                     ALTFLATEPSQVLGSTDLAAVPLFLMMGAFVNVSGFSRDLYAAAAALLGHRRGGLSYA
                     TIGGSAAFGAICGSSPATVATFTQVALPEMLKRKYSPGFSGATIAAGGALKALIPPSL
                     SMILYCVVARTYIFDVFIAAVIPALITIAANLIAIAVMTRLDPAIAPVSGRVSRQEKL
                     AAVRRAAPAALLIAIIFAGLYSGVFTVNEAASVAAVVSFAFALMRRTITPGNLVSSLR
                     ETALIAGMIYFVLIGSSIFTYFISLAKIPEQLIAFLGHVDLPPIGIIVLMLAAYLVLG
                     AVFDELAAMIVTLPFALPVIVYFGYDPVWWAIINVVIVELGLVIPPIGLVILIINAMR
                     PDIPLRALYRNIVPFVVADLVVLALLTSFPSLALWLPALLRG"
     gene            5552..6556
                     /locus_tag="VGA15_20035"
     CDS             5552..6556
                     /locus_tag="VGA15_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9590023.1"
                     /translation="MSLPTTNFDPGFHITRASHVVLTVRDLTASRQFYQEVIGLILTA
                     EEDDTLYFRGVEEACHHSLVLRKAKDASCMRIGLRVFTDGDLDRLKAFFEAHGCPAAW
                     VDVPHQGRTLHATDPTGTPLEFCATMPVMPRMITKFTRHAGGSALRLDHYQILTPDVR
                     KVSEFYMAAGFRLSEYIAPAGTFDLRGVFLQRKGNPHDIVFFNGTGPRLHHFAFLAPE
                     VHHLLYACDVAGELGFGTRVERGPGRHGPGHAMYVYMRDPDGHRVELFNTHYQIMDIE
                     NEPVGWDPSDPGIAHPWGLPARQAWFEEATPFAEVATREPQVRPSPMTLEKYLAKQNA
                     "
     gene            6568..7266
                     /locus_tag="VGA15_20040"
     CDS             6568..7266
                     /locus_tag="VGA15_20040"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX9590024.1"
                     /translation="MPETALDTPESAKETLASHVYDRLRQDIISVAIEPGEKLHIRSL
                     CERFDVGLSPMREALSRLSSEGLVAQSDHRGFAVAPMGEVDLVDITRARCWLNELAIR
                     KSIAHGDAAWEEQVVLSFHRLSRTPRYEPGKEQERSPAWEIAHRNFHSSLISASGSRR
                     LEQYCEHLFDSSERYRHVGRKAGIKGENREKEHRALMEAVVARDADAAARLITAHFER
                     TAELVRQVLKGRAR"
     gene            7263..8840
                     /locus_tag="VGA15_20045"
     CDS             7263..8840
                     /locus_tag="VGA15_20045"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HEX9590025.1"
                     /translation="MIQTPNYDVAIVGYGPVGAIFANLLTNYGLKIAVIERAAAIYDK
                     PRAITLDHEALRVFQACGLADVMENAIAPHNGSHYLGIDREVIKMFDPMPPPYPLGWI
                     PNATFVQPDAEQALRDKLAGYQDADAFLSTTGVSLSQDEASVSLKARRETGDEFDISA
                     RYLVGCDGANSFVRKHLGIALEDLAFDEWWMVVDTLSSEPAKRPAKSFQYCWPSRPGT
                     FVPGPRNLRRWEIKLLPGEDPELAGAPDNVAKLLEGFTDTSDLTIWRSAVYRFHALLG
                     ESWRDRRVFLMGDAVHQTPPFLGQGLCAGIRDASNLAWKLALVLRGSAGDTLLDSYEV
                     ERKPHVRSVVASAKEFGKIIGELDPAAAVARDERLRAELKSGKAETIRQKFIPDLTCG
                     LIASRNKLGGRLFVQPRVRSADGTVRRLEDLLKSEFAVVTATREAMSWMSEASLLCWQ
                     QLGGERVVVTSAGESSTRDGISTVVEIDGVFSNWMRENQVGAVIVRPDRYVFAGAGNA
                     DQLKMLMGNLIEALRGR"
BASE COUNT         1937 a         3130 c         3192 g         2205 t
ORIGIN      
        1 caaaaagaag ctcggccgtt cgtttgaggt ctcgaccatt gatctccatc cgatgtggct
       61 ccttgcgatt ccgcttcagc gtcgggcacg aaaagcaatc ccgacgatac catacctctt
      121 tcaacgtcca gagttgtgcg catctagttc ggcaaaaata tttctcgatt gttgatcgga
      181 ggcccgagtc gaatcggccc ggggcagggc gcggtgagca agataaagct ggagttccgc
      241 gccgccgggg caagatcatc aacatcgcat ccgtgcagag cgaactcgcg cgtccgggca
      301 tcgcgcccta cacggccacc aagggcgcgg tgaagaacct gacccgctgc atgtgcgccg
      361 attgggcaca atacggcctc cagatcaatg caatcgcgcc gggctacttc aacaccccgc
      421 tcaaccaggc gctggtcgac aatccggaat tctcgtgcgg actcgaaaag cggacaccgg
      481 ccgcccgctg gggcaatgtc gcggagttgg tgggcgcagc tgtgttcctg tccggtaggg
      541 cctcgtcctt cgtcaacggc catacgctct gtgtcgatgg cgggatcacc acctgtctct
      601 aggcttgcgc cgcttcaagc ctctcgttga actgcgtgag atgaattgcc gcccgatcac
      661 atccgtcgta tctggagtgc aacaatgaaa gccgtcgtca ttcatgctgc ccgcgatctt
      721 cgggtcgagg agcgggagcc cgaggcagcc ggtgcgggac aggtcgaggt cgccgtcgag
      781 gccggcggta tctgcggatc ggatctgcat tatttcaacc atggcggctt cggcacggta
      841 cgggtgcgcg agccgatgat cctaggtcac gaagttgccg gaaccatcaa ggcgctgggt
      901 gcggccgtat ccggactcgc gatcggcgac cgcgtcgcca tctctccgag ccggccttgc
      961 aatatttgta actattgctt gaagggccag caaaaccagt gcctcaatat gcgcttttac
     1021 ggcagcgcga tgcctatgcc gcatatacag ggagcctttc gccagcgtct cgtcgccgaa
     1081 gcttggcaat gccacaaggt cgccgacggt gtctcgatca acgaagctgc ctttgcggag
     1141 ccctttgccg tcgtcctgca tgccgtcagc cgtgccggat ctctactcgg caaacgggtc
     1201 ctcgtgacgg gatgcggtcc gatcggtatg ctggcgatca tggctgcccg gctccacggt
     1261 gctcgggaaa tcgtcgcgac cgacgttgtg gccgcagttc tggagaaggc gctgcggctc
     1321 ggcgctgatc gaaccatcaa tgtcgctgat gacccccatc agctcggcac ctattctgcc
     1381 aacaagggct atttcgatgt ccagttcgaa gcgtccggca acgaacgagc cgttcgctcg
     1441 gggctcgagg tgctcaagcc gcgcggcatc ctggttcagc tcggcctcgg cggcgatgtt
     1501 tcgatcccgc agaacatggt ggtggccaag gagatcgaaa tgcgcgggac ctttcgcttt
     1561 cacgaggaat ttggtctggc cgtcgacctg atcaaccaga ggagagtgga tctgacgccg
     1621 ctgctgaccg gcgtctttgg actggacgag gccatacacg ccttcgagat cgcgggagat
     1681 cgcagtcgat ccatgaaggt tcagctaaga ttctgaagct gcagcgattc ttagatcagc
     1741 gcaggatatg atccgccgtc gagatggagg ttctggcccg acataaagcc cgccaaaccg
     1801 ctacaaagga aggcgcaggt cgcgccgaat tccttgggat cgccgagccg tctcgcggcg
     1861 atcgattcga tctgacgcgc gcgggcttcc tcgtaggaga tatcttcgcg cgccatcgcg
     1921 gccttcgcca tctggtgctg gcggtcggtg tcgaaccgct cgggcagcag gttgttgatc
     1981 gtcacgttgt cgcgcgcaac ttcgagggaa agccccttca tcgccgcggt caggccgccg
     2041 cgcgcgccgg aggacaaggt catatgcggg cggggtgtcg ttaccatggc ggatgtgatg
     2101 ttgacgatgc gcccgaagcg ccgctgtctc atggccggca gcaccgcgcg gatcagcatc
     2161 agcggcgcga tcatgttggc ctcgatcgtc gccagccagc ggtcgcgctc gatgtcggcg
     2221 aaattgccgg gcgagggacc tgcattgttg ttgacgagaa tgtcgggcga gggacacgcg
     2281 gcgagcagcc gtgcctgacc gtccgttgtg gtgacatcgg ccgcgaccgc gatgacgtcg
     2341 ccgcgtgccg cttcccgcaa ttctgccgcc gtcctgtcga gcttcgccgt atcgcgcccg
     2401 ttgatgacga cgtgggcgcc ttcctgcgcc agagcctgcg cacaggcgag cccgagcccc
     2461 tggctagatg cgcaaaccag cgcggtgcgc ccttcaatcc ccagatccat tttctcgctt
     2521 cctgagctta ttatttgaaa tcacttggct ttctgtcgaa tatcacggca tttcgctatc
     2581 tgaataatcg attatggcaa aaattactat tgaaaatcga ttatttttcc acgatgactc
     2641 ctctcaacaa aagtgcggcc accggcgaag tcgatccagc cgcagcatca tagggaggag
     2701 aagatgacgc gattttgcat ggcactgctg gcgtggttcg cggtgttcac gccgctgaca
     2761 gcaggcgcag agccagtaac cctcaagctg tcgttcttcg cttccgagac cgaggtcaat
     2821 tacgcgcgtg ccatcaagcc atggatcgac gccgtgaacg ccgatccgag cggcgcggtg
     2881 aagatcgagg cctatccgaa tggcgcgctc ggcaaggcgc tgccggcgca gccgcagctt
     2941 ttgctcgacg gcgtcgccga catcgccttc gtcaatccga gccttgggcc cggccgcttt
     3001 ccggacgatc aggtgttcga agtgccgggc ctgctgcaga atctgaaaga gggcgtgaag
     3061 ctctacgaag agctgctcaa ggcaaatctc ttgcgcggct attccgacta ttacgtcgtc
     3121 ggcgccttca tgaatccgca atacatggtc ttcgcccgcc gcccgatcaa atcgctcgcc
     3181 gatctcaagg gcatgaaggt acgcatcaac ggcgcggtga tcggccagac cgtcaaggag
     3241 cttggcatgg tgccggtctt gatgccaccg aacgaggtcg tcgaggcgat gggacgcggc
     3301 acgatcgacg cgaccaccac cgtgcctgcg gcgatcgtcg atttcggcat cgaccgcgtc
     3361 accagccacg actatttgct gccgctcggt gcaggccccc tggccgtcct gatgaatcgc
     3421 aagaagttcg agtcgctgcc gaagccggcg caggacgtga tcgcgaaata ttcgatccgc
     3481 tggatcgacg acacctatat ccaggacctc gggcaataca acgacgagct gatcgcgagg
     3541 ttcaaggctg atcccaaacg cgtcgtgacg tctccgtcgc aggctgacat cgccagcctg
     3601 cagccgattt atctgaaagt caccgctgaa tgggtcgcca agagcccggg gaatgccgag
     3661 ctgctgggca aggcgcgtga gttgctggcg gggatcaggg ccaactagag tgaaggcggc
     3721 gcgaaccatc gtcaccggca ttgcgaacgc gttcgccgtc gccgggttgt cgttgctact
     3781 gattcttgcg gtcttcacgc tgctcgacgg gttactcagg gcggttgcga acacgccgat
     3841 cgacctggtg cgcgagatcg gcgacatggt cgcggcgatc tgcggcacct gctgcctgcc
     3901 gatcgtgctg ctgcaccaaa aaaatatcgt gctgcggatc ttcgatcgaa ttctgccgcc
     3961 atcaggtgtt cgcgtgatcg acacgatcgc cgcgctggcg atcgagatcg tcatgatcgg
     4021 catggcctgg caattctggc ttttcggcat caagacgatg cgggcaggcg acgtcacctg
     4081 gctgctcaac tggccgaagg cgccgttctg gttcgctgtc gacggcattc tctgggcggc
     4141 ggtggtcgtg caggctttcg tgttggtcga ggagatgacg ggcgccggcc ggcaacgcgg
     4201 tccggagacc gtggcatgac ggcgaccggc atcggcatcg gcggcatcgt ggcgctgttc
     4261 gtgctggtcg cgctccatat tccgctggcg tttgcaatga tcgccgtcgg catcgtcgcg
     4321 ttcgcacttc agaccgactg gtcctcggcg ctgacctttc ttgccacaga gccgtcgcag
     4381 gtgctcggca gcacggacct tgcggccgtg ccgttgttcc tgatgatggg cgcgttcgtg
     4441 aacgtctcag gtttttcgcg cgacctctac gcggcggctg cggcgctgct cggccaccgg
     4501 cgcggtgggc tgtcctatgc gaccatcggc ggcagtgccg cgttcggcgc gatctgcgga
     4561 tcgtcgccgg cgacggtcgc gaccttcacg caggtggcgc tgccggagat gctcaagcgc
     4621 aaatactcac cgggtttttc gggggcgacc atcgccgcgg gcggtgcgct gaaggcgctg
     4681 attccgccct cgctcagcat gatcctctat tgtgtggtgg cgcggaccta cattttcgac
     4741 gtcttcattg cggcggtgat tccggcgctg atcaccatcg ccgccaacct gatcgccatc
     4801 gccgtgatga cgcgccttga tccggcgatc gcgccggttt cgggacgggt atcgcgacag
     4861 gaaaagctcg ctgccgtgcg gcgcgccgcg cccgcggcac tgctgattgc gatcatcttc
     4921 gccgggctct acagtggcgt cttcaccgtc aacgaggcgg cttcggtcgc agctgtcgtg
     4981 tcgtttgcgt tcgcactgat gcgccgcacc atcacgccgg ggaatctcgt ttccagcctg
     5041 cgcgagaccg cgctgatcgc cggcatgatc tatttcgtgc tgatcggctc ctcgatcttc
     5101 acctatttca tcagccttgc caaaatcccg gaacagctta tcgccttcct cggccacgtg
     5161 gatctgccgc cgatcggcat catcgtgctg atgcttgccg cctatctggt gctcggcgcc
     5221 gtgttcgacg agctggccgc catgatcgtc actctgccgt tcgcgctgcc agtcatcgtt
     5281 tactttggct atgatccggt ctggtgggcg atcatcaacg tggtcattgt cgagctcggc
     5341 cttgtcattc cgccgatcgg ccttgtgatc ctgatcatca acgcgatgcg gccggacatt
     5401 ccgttgcggg cactttaccg gaacatcgtt ccctttgtag tggccgatct cgtcgttcta
     5461 gcgttactga cttcattccc gtcattggcg ctgtggctgc cggccttgct gcgtggctga
     5521 ctatgataag atgaaacgaa aggtgagaga aatgagcctc ccaaccacga atttcgatcc
     5581 gggatttcac attacccgcg cgagccatgt cgtccttacc gtccgcgacc tcacggctag
     5641 ccggcaattc tatcaggagg tgattggcct gatcctgacg gcggaggagg acgacaccct
     5701 ttacttccgc ggcgtcgagg aggcgtgcca ccacagtctc gtgctgcgta aggcaaagga
     5761 tgcctcgtgc atgcggatcg ggctgcgcgt attcacggac ggcgatctcg acaggctcaa
     5821 ggcatttttc gaagcccatg gctgtccggc cgcgtgggtt gacgtgccgc atcagggtcg
     5881 aacgctgcat gcgaccgatc cgaccggaac gccgctggaa ttctgtgcga cgatgccggt
     5941 gatgccgcgc atgatcacaa aattcacacg ccacgccggc ggctcggcgc tgcggctcga
     6001 ccactaccaa atactgacgc cggacgttcg caaggtgagt gagttctaca tggcggcagg
     6061 gttccgcttg agcgaatata tcgcaccggc aggcaccttc gacctgcgcg gcgtgttcct
     6121 gcaacgcaag ggcaacccgc atgacatcgt gtttttcaac ggtacgggtc cgcgcctgca
     6181 ccattttgcc ttcctggcgc cggaggtgca tcatctgctg tatgcctgcg atgtcgcggg
     6241 cgaactcggc ttcgggactc gcgtcgagcg cggcccgggg cgacacgggc cggggcatgc
     6301 gatgtatgtc tacatgcgcg acccggatgg ccatcgcgtc gagttgttca atacccacta
     6361 ccagatcatg gatatcgaga acgagccggt tggctgggac ccttcggacc ctggtatcgc
     6421 ccacccctgg ggattgccgg cacgccaggc ctggttcgag gaggcaacgc ctttcgctga
     6481 agtcgctacc cgcgagccgc aagtcaggcc aagccccatg acgcttgaga aatatctcgc
     6541 aaagcagaat gcctaaaggc cagtccgatg cccgagaccg cgctcgatac ccccgaaagt
     6601 gccaaggaga cgctggcctc gcacgtctat gaccggctgc ggcaggacat catctcggtt
     6661 gcgatcgagc cgggcgagaa actgcacatc cgatcgctgt gcgaacgatt cgacgtcggc
     6721 ctaagcccga tgcgggaggc gctgagccgc ctgtcgagcg aggggctggt cgcgcagagc
     6781 gatcaccgcg gttttgcggt tgcgccgatg ggcgaggtag acctcgtcga tatcacccgt
     6841 gcgcgctgct ggctcaacga gcttgcgatc cgcaagtcca tcgcccatgg cgatgccgca
     6901 tgggaggagc aggtcgtgct ttcctttcat cggctgtcgc gcacgccacg ctacgaaccc
     6961 ggcaaggagc aggaacgcag ccccgcgtgg gaaattgccc atcgcaattt tcactctagc
     7021 ctgatctcgg cctcgggttc gcggcggctc gaacaatatt gcgagcattt gttcgattca
     7081 tccgagcggt accgccatgt cggccgcaag gcgggcatca agggcgagaa ccgcgagaag
     7141 gaacatcgcg cgctgatgga ggctgtggtg gcacgcgacg ccgatgccgc ggcacggctg
     7201 atcacggcgc atttcgagcg caccgccgaa ctggtccgcc aggttctgaa gggccgcgcg
     7261 cgttgatcca gaccccgaac tatgacgtcg cgatcgttgg ctatgggccg gttggcgcca
     7321 tattcgccaa tctgctgacg aactacggcc tgaagatcgc ggtgattgag cgcgcggctg
     7381 ccatctatga caagccgcgc gcgatcacgc ttgaccatga ggcgctgcgc gtgttccagg
     7441 cctgcggtct tgccgacgtc atggagaatg cgatcgcgcc gcataacggc tcgcattatc
     7501 tcggtatcga ccgcgaagtg ataaaaatgt tcgatccgat gccgccgcct tatccgttgg
     7561 gctggattcc gaatgcgacc ttcgttcagc ccgacgccga gcaggcgctt cgcgacaagc
     7621 tcgccggata ccaagacgcg gatgcgtttc tctcgaccac tggcgtatct ctgtcgcagg
     7681 atgaagcatc ggtatcgctg aaggcgagga gggaaaccgg cgatgagttt gatatcagtg
     7741 cccgctatct ggtcggatgc gacggtgcca acagtttcgt ccgcaagcac ctcggtatcg
     7801 cattggagga tctcgccttc gacgaatggt ggatggtagt ggacacgctg tccagcgagc
     7861 ccgccaaacg gcccgcgaag agttttcaat attgctggcc gtcacgtccc gggacgttcg
     7921 tgccgggccc ccgcaatctg cggcgatggg agatcaagct gctgccgggc gaagatcccg
     7981 aactcgccgg ggcacctgac aatgtggcca agctgctcga aggtttcacc gatacgtccg
     8041 atctcacgat ctggcgctcg gcagtctatc gcttccacgc tttgctcggc gaaagctggc
     8101 gagaccgtcg cgtgttcctg atgggggatg ccgtgcacca gacaccgcca ttccttggac
     8161 agggcctctg cgcgggcatt cgagatgctt ccaatctcgc atggaagctg gcgctcgtgc
     8221 tgcgcggcag tgccggtgac accttgctcg atagctacga agtcgaacgc aagccgcatg
     8281 tccgctcggt tgtcgcgagc gccaaggagt ttggcaagat catcggggaa ctggaccccg
     8341 cagcggcggt tgcccgcgat gagaggttgc gcgcagaact caaatcagga aaagccgaga
     8401 ccattcggca gaaattcatt cccgatctga cctgtggcct gatcgcctct cgcaacaagc
     8461 ttggtggtag gttattcgtt caaccgcgcg tgcggtcagc ggacgggacc gtgaggcgtc
     8521 ttgaggacct gctgaagtcg gaatttgcgg tcgtcacagc aacgcgggag gcgatgtcgt
     8581 ggatgtcgga agcgtcgctt ttgtgctggc aacagcttgg cggtgaacgc gtggttgtca
     8641 ctagcgccgg ggaaagctca acgcgagacg gcatctcgac cgttgtcgaa atcgacgggg
     8701 tattttcgaa ttggatgcga gaaaatcagg tcggcgccgt catcgtgcgg ccggatcgct
     8761 atgtcttcgc cggtgccggc aatgcggacc aactgaaaat gttgatggga aacttgatcg
     8821 aagcgctgcg tggtcgatga cagccttctc aaagcagttc tctgtcagaa ctcgcgataa
     8881 cgccgttgcc tacgctgaga cgacacccat tctcccttcc aaaacagacg gtgcatggag
     8941 gcaatgcgca ctagatgcga tccgaggcct ccggaacgat cgcgcaatgc gcattgatgg
     9001 cacgtttgag acatgccgac cggcttggga attgtccgct tatcggggaa gaccggaagt
     9061 catcggcgta ccctcagaat tacacgactg acccgattca gacatgacgg tgtccgtgat
     9121 gccttgctct gctcgatact ttgctcgacc ggctgggatg tggtgtcgcc aggacgcaat
     9181 ttcagtcggg aacaccagct cgggaactgc aatccgtgct caagggcggt ttgcttttgc
     9241 ataagaaccg aaaaaattct tcattcccgc agaaaagtta gtccacctgc gcgataagga
     9301 tgcatcttcg cagcgcgaac cttttgatcg tgttgccgag ctacccgctc tcgggatcgg
     9361 aacgttagtc gaactgatcg ccatgggtgg cccgggcgaa gatatcgagc ccgttagacg
     9421 atcgtcctga acatctgctt gcgcgctttg gctctgtcgt tctgcaatgg ctgctgcgac
     9481 tgcttctcgt ctttgggtat ccaaatacaa cgtgtactct tcgtcgaagt gtttcagttc
     9541 gttcggtgtt gggttcggtc ccgcgatatc gaaatggcag ttttgcagaa agtcataata
     9601 tgtgtatccg ccgccattct tgcgtcggcc tacgaactgc ttgtcacact gagctatcaa
     9661 cgagatgcgc ttctcttttg atgatgcggt cctctgcttt ctcagagcat tgttcaaaat
     9721 cgtgggggtt gtgctgaggc caccactgga cgctaaacca cgttgccacg ctgacggcgc
     9781 agcagatgaa tgcgattgcg acaagcctgg atttgaattt ggctctcacg tagctttcca
     9841 cgcagacaat gcaccgctgt tccatacaaa ccgaatcttg attccgtgcg gatattgtca
     9901 ctcgtcaacc tcaaattctc caaaaaaatc gctgccattt ggccttgcgg tctctggttg
     9961 tgcagacctt ctaaaaccac ctaatgttgc ggcgcagcca cagcccgatc gattacttgt
    10021 ttggaactgc aggagaaggg ctggagctac gtccggcctt aacaaactta ccttcggcga
    10081 cgccaagaaa gtcgattttg agcatcttgg ttgctgtcgg tggcccactt acatgccaat
    10141 ccaattttct tccggacgct atccaatccg gtgagttgga acgttgcttc ttggccgagg
    10201 ccctgcctat cgaacacgct gattttcagc cgtgcgtttt ctggtagcga ccgcagaagt
    10261 ccaactgcat cgtctttgta actagcggtc tttccgtccg cggacacagt ccactgtagc
    10321 ctaacgaagg gttggtcgtt gatctgatag tcgacttgaa cttcgctggc gcgcgacgcg
    10381 ggccacgttc cctccatgcg aagcaggagt tcggtgcgta gtccacgaca gcgaacagct
    10441 agagtgtttg gtgcatcctt cacg
//
LOCUS       DASWGM010000715         6826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1174275,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000715 DASWGM010000000
VERSION     DASWGM010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174275"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VGA15_20050"
     CDS             <1..502
                     /locus_tag="VGA15_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX9590026.1"
                     /translation="ALAAKFVVCPSLDQSERQTIDDWKRVCENLNSIGELATHSGLTF
                     AYHNHSFEFSNLPNGVSPYDFLLAHTDDRFVKFELDVYWAKKGNVDPVTYLKAYQSRF
                     RLMHLKDMATDGSFTEIGNGTLDFDEIIAASIAAGVRHFFVEQDYSSDPMQSITQSIA
                     YLQRYR"
     gene            complement(894..1598)
                     /locus_tag="VGA15_20055"
     CDS             complement(894..1598)
                     /locus_tag="VGA15_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590027.1"
                     /translation="MLKLSGVSASYGSVPAIGNVSIEIGEGEAVGLLGANGAGKSTSL
                     RVISGLVKLTSGTITFAGTNLASLPPHKIPELGIAHVPEGRQVFPEMTVQENLEIGAY
                     VPKAKAERSRTLDLVYSIFPRLADRKKQLAGTMSGGEQQMLAVGRGLMLKPRLLMLDE
                     PSLGLAPVMTDVTFEKIGEIHKMGTAILLVEQNVSRALSLVQRAYVLESGNVIMHGTS
                     AELAGNKQVQAAYLGI"
     gene            complement(1598..2341)
                     /locus_tag="VGA15_20060"
     CDS             complement(1598..2341)
                     /locus_tag="VGA15_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590028.1"
                     /translation="MADALVIRGLNKRFGGLRAVQDVSFTVQENETVALIGPNGAGKT
                     TSFNLITGFHRPDSGSVLAYGREITGLKPHDICAHGLARTFQVAKPFGAMTVLANVMT
                     GAFLRDKNTAAARDQARDAIEFVGLSAKEQTAAKDLTTIDQRRLEMARALATQPRLLL
                     LDEVMAGLNPSEIDQAVALVGKLSKRGLTIVIVEHVMRAIMAVARHIVVLDHGQKIAE
                     GAPKEIVENPEVIRAYLGSYVHPPTPGET"
     gene            complement(2351..3343)
                     /locus_tag="VGA15_20065"
     CDS             complement(2351..3343)
                     /locus_tag="VGA15_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590029.1"
                     /translation="MTAQGVRTRGPFWLAIGIAVVVALPLVVKSSFAIDIFIRILLFS
                     FIGVAWNLMGGYAKQLSLGHAAYFGLGAYTSTILQVDFGISPWIGMIAGGVVAMLASL
                     PIGWLCFRLRGPYFTIATIATAQALMLIFLKFRDLAWGAEGTTIPNLGSAPLMMQFDT
                     KAAYYYVALALLALGLVITYLIERSWMGYYLVAIGEDEDAAEAIGVNAPKIKRDIYLI
                     SSFLTALAGTFYTQYIYFIDPATAFSFNISIEAALVSIVGGIGTLWGPVIGTVLLETT
                     STLLQSWLGGSIGGVQLTVYSLILMAVILWRPTGLIGVATDAWQRYVSRKPVRA"
     gene            complement(3340..4197)
                     /locus_tag="VGA15_20070"
     CDS             complement(3340..4197)
                     /locus_tag="VGA15_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590030.1"
                     /translation="MAQAVINGLLIGGIYALVSIGVTLIFGVVKIVNFAQGEFVMIGM
                     YISFFLANQFGIDPLVSLLVSMPTLFVIGVLIQHFLIRRVLGPNDMPQIFLTFALSLL
                     LLNLALMLFSANYRTVHTSYSDEAIHIGELYIPVAKLIAFVVAMALSGALWVFLHFTD
                     LGKAMRAAAQNRDVATLMGINPNRVFCVALGIALALAGAAGSLLMPFYSAYPFVGQVF
                     VLMAFVAVVLGTLGNVIGALIASLMMGVAESLGIQYIGADSGLIVVFAMLLLTLAFKP
                     TGLGGGRAR"
     gene            complement(4222..5475)
                     /locus_tag="VGA15_20075"
     CDS             complement(4222..5475)
                     /locus_tag="VGA15_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9590031.1"
                     /translation="MKKDRQHSVSNKADLSRALSKRQGRREFLLKTGGILAAGAFGAV
                     PMRGWAADPVNIGALYPTTGSMAQIGVGCVAAAKLAAEMVNDAGGIKSLGGAKLNLIL
                     SDVQSDTTVTRTETDRLITGNKLSAIHGCFASALTLIASEVAERAKVPLLTGSSSDQL
                     NKGRTYTFTPFARASQFALAQLRMSRLVSDKPKVAVVFENTAFGTSTSNGLREQATAE
                     GVEIVLFEPYSAGFTDASPLINKVKSSGANALFSVSYLNDLILIVRTVKQVGLNIAIN
                     GGSGGFVIPDFYKNVGKLAEGLQGVAHWNHDMDANAEKVNAEYKKRTGEFLFEYAGGL
                     VAQTFMIADALERAASTDPQKVRDAIASLDVSAGYAAMCPGGKVKFGPDGKNIYGHPV
                     GVQWQNGDLASVFPEEDKRAPLMKT"
     gene            5660..6400
                     /locus_tag="VGA15_20080"
     CDS             5660..6400
                     /locus_tag="VGA15_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9590032.1"
                     /translation="MVGKPAKHAIARTPKSRQMTISPAREPAAAVIAKSIEEDIVLGR
                     RQPRERLVEQDLCDLFQTHRGDVRLALFELEKKGLVERIPNRGALVRGLTPLEVKEIY
                     AVREELEVMAVRIIPFPVGSSDIERLEELQVQHSAAVAAGDLLTVFYSNLSFHQVLFG
                     LCGNACLIETIDLLAQKVYGIRSYANAFPESLDRARQDHLDMIKALRGSHRDDLIVLA
                     RRHLEPSPQAYIKAYERRFGKTDLTPAH"
     gene            complement(6457..>6826)
                     /locus_tag="VGA15_20085"
     CDS             complement(6457..>6826)
                     /locus_tag="VGA15_20085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9590033.1"
                     /translation="EIGDHEKSDFLSAAIGLLLPIDWPEPFGLVMIEAMACGTPVIAY
                     NHGSVPEIIDEGLTGFVVEDEASAVGAVDRLSRLSRHAIRKQFEARFTARRMALDYLA
                     AYRSLMEAAEPRIKLVSSAE"
BASE COUNT         1367 a         2025 c         2098 g         1336 t
ORIGIN      
        1 ggcattggcg gcgaagtttg tggtttgccc aagcctggac cagtcggagc gacaaacgat
       61 cgatgattgg aagcgcgttt gcgaaaacct gaactccata ggtgaactgg cgacgcattc
      121 gggattaaca tttgcctatc ataatcacag ttttgaattc tcgaatttac ccaatggagt
      181 cagcccctat gatttcttgt tggctcatac agacgatcga tttgtgaaat tcgaactgga
      241 cgtctattgg gcaaaaaagg gcaatgttga tcctgttact tatttgaagg cgtatcaatc
      301 gcgatttcgt ctgatgcatc tgaaggacat ggcgacagat gggtccttca ccgaaattgg
      361 caatgggaca ctggatttcg atgaaatcat tgccgcttcg atagctgccg gagtgcgcca
      421 tttcttcgtc gaacaggatt attcgtcgga tccaatgcaa agcatcacgc agagtatcgc
      481 ttatcttcag cgctatcgat gataaggctg ggccaagcga acccaaacaa gagatgttgg
      541 gcaaaccgcg cgcgacacgg tgaaagagat ggattacgcc ggagcctgtc atcgggcagc
      601 gccttgcgca ggacccgttg cgctaacccc gccatccggg ttacgccgct tgcgcgcacg
      661 gcggaatttg ttgaggtgaa tcggcggctg tttgaaaatt gaatccgcat gtgtcgtccc
      721 cgcgaacgcg gggacccatc acccctggca gtcgtgacag cgacgcgatg gtccagcaag
      781 gacactgacg ccatcgcgga tcatcgacga cacggcgtat gggtccccgc tttcgcgggg
      841 acgacggtga gagattacgt atgtcgcgtc ttgtcgttgc gatgtaccgc gtgtcaaatc
      901 ccgagatacg ccgcctgcac ctgcttgtta ccagcgagct ccgcgctggt gccgtgcatg
      961 atcacgttgc cgctttccag cacataggcg cgctgcacca gcgacagcgc gcggctgacg
     1021 ttctgctcga ccagcagaat ggcggtgccc atcttgtgga tttcgccgat tttttcgaag
     1081 gtcacgtcgg tcatgacggg ggccaggccc agcgagggtt cgtcgagcat cagcagccgt
     1141 ggtttcagca tcaacccgcg gccgaccgcc agcatctgct gttcgccgcc gctcatggtg
     1201 cccgcgagtt gcttcttgcg atcggccagt cttggaaaga tgctatagac cagatccagc
     1261 gtgcggctgc gctcggcctt ggccttcggc acgtaagcgc cgatctcgag attctcctgc
     1321 accgtcattt ccggaaacac ctgccggccc tccggcacat gggcaattcc caattccgga
     1381 atcttgtgcg gcggcagcga cgcgagattg gtgccggcga aggtgatggt gccggatgtg
     1441 agcttcacaa ggcccgagat cacgcgcaag gaggtgctct tgccggcgcc gttcgcgccc
     1501 aaaaggccga cagcctcacc ttcgccgatc tcgatgctga cattgccgat ggcgggcacc
     1561 gagccgtagc tggcgcttac gccggatagc ttgagcacta ggtctctccc ggtgtcggcg
     1621 ggtggacgta ggagcccaga taggcgcgga tcacctccgg gttttccacg atctccttcg
     1681 gcgcgccttc ggcgatcttc tggccgtgat cgagcaccac gatatgacgg gcgacggcca
     1741 tgatggcgcg catcacatgt tcgacgatga cgatggtcaa gccccgcttg gacagttttc
     1801 cgaccagcgc caccgcctga tcgatctcgg atggattgag cccagccatg acttcatcga
     1861 gtagcaggag ccggggctgc gttgccagcg cgcgcgccat ctccagccgg cgctgatcga
     1921 tcgtggtcag atccttcgcc gcggtttgtt ctttggccga gagaccgacg aactcgatcg
     1981 catcacgcgc ctggtcgcgc gcagcggcag tattcttgtc gcgcaaaaat gctccggtca
     2041 tgacgttggc cagcaccgtc atggcgccaa acggttttgc gacctgaaag gtgcgcgcca
     2101 ggccgtgcgc gcagatgtca tgcggcttca atcccgtgat ctcgcggcca taggccaaga
     2161 ccgaacccga gtcgggccgg tgaaatccgg tgatgaggtt aaagcttgtg gtcttgccgg
     2221 cgccgttggg cccgatcagc gcaacggttt cgttttcctg caccgtgaag ctgacgtctt
     2281 gtacggcgcg caaaccgccg aaccgcttgt tgagacctcg aataacgagc gcgtctgcca
     2341 tgggatgccc ttacgctcgc acaggcttgc ggctgacata gcgttgccag gcgtcggtcg
     2401 cgacgccgat cagtccggtc ggccgccaca gaatgacggc catcaggatc aggctgtaga
     2461 cggtgagctg cacgccgccg atcgaaccgc cgagccagct ctgcagcaaa gtcgacgtcg
     2521 tctccagcag gacggtgccg atcaccggac cccacaacgt cccgattccg ccgacgatcg
     2581 ataccagggc ggcctcgatc gagatattga agctgaacgc ggtcgccgga tcgatgaagt
     2641 agatgtattg ggtgtaaaac gtgccggcga gggcggtgag aaacgaactg atcagataga
     2701 tgtcgcgctt gatcttcggt gcgttgacgc cgatcgcttc ggcggcgtct tcatcttcgc
     2761 cgatcgcgac gaggtaatat cccatccagg atcgctcgat cagataggtg atcaccagtc
     2821 cgagtgcgag caaagcgagc gcaacatagt aatacgccgc cttggtgtcg aactgcatca
     2881 tcagcggcgc gctgccgagg ttggggatgg tggtgccctc cgcgccccag gccagatcgc
     2941 gaaacttcag gaagatcagc atcagcgcct gcgccgtcgc gatggtggcg atcgtgaagt
     3001 aggggccgcg caggcggaag cacagccagc cgatcggcag gctcgccagc atcgccacca
     3061 cgccgccggc gatcattccg atccatggcg agatgccgaa atcgacctgc aggatggtcg
     3121 aggtgtaggc cccgagcccg aaataggcgg catgccccag cgaaagctgt ttggcgtatc
     3181 cgcccatcag gttccaggcg acgccgatga aagagaacag caggatccgg atgaagatgt
     3241 cgatcgcaaa cgagctcttt acgaccagcg gcaatgcgac gacgaccgcg atgccgatcg
     3301 ccagccaaaa cgggccgcgc gtgcgtactc cctgcgctgt catcgcgctc tcccgccgcc
     3361 aaggccggtc ggcttgaagg caagcgtcag aagcagcatg gcgaacacca cgatcaatcc
     3421 ggagtccgcg ccgatgtatt gaatgcccag cgactccgcg acgcccatca tcaggctggc
     3481 gatgagcgcg ccgatcacat tgccgagcgt gcccagcacc acggcgacga aggccatcag
     3541 cacaaacacc tgcccgacaa acgggtaggc ggaatagaac ggcatcaaca gcgaacccgc
     3601 cgcacccgcc agcgccagcg cgatgccaag ggcgacgcaa aatacgcggt tgggattgat
     3661 gcccatcaat gtcgcgacgt cacggttctg cgccgcggca cgcatcgcct tgccgagatc
     3721 ggtaaagtgc aggaagaccc agagcgcgcc gctcagcgcc atcgcgacga caaaggcgat
     3781 gagttttgcc acgggaatgt agagttcgcc gatatggatg gcttcatcgg aatacgaggt
     3841 gtgaacggtg cggtaattcg ccgagaacag catcaacgca aggttcaaca gcaacagcga
     3901 cagggcgaag gtcagaaaaa tctgcggcat gtcgttcggc cccaacaccc ggcggatcag
     3961 aaaatgctgg atcaggacgc cgatcacgaa cagtgtcggc atcgagacca gcagcgacac
     4021 caggggatcg atgccgaact ggttggcgag aaagaagctg atatacatcc cgatcatcac
     4081 gaattcgccc tgggcgaagt tgacgatctt gaccacgccg aaaatcagcg tgacgccgat
     4141 gctgacgagc gcatagatgc cgccgatgag caggccgttg atgacggcct gggccactgt
     4201 ctcccacatc gttttgacgc ttcaggtttt catcagcggt gcgcgcttgt cttcttccgg
     4261 aaagacgctc gccagatcgc cgttctgcca ctgcaccccg accggatggc cgtagatgtt
     4321 cttgccgtcg ggtccgaact tcaccttgcc gcccggacac atcgcggcat aacctgcgga
     4381 tacgtcgagc gatgctatgg catcgcgcac cttttgcgga tcggtggagg ccgcccgctc
     4441 cagcgcgtcg gcgatcatga aggtctgcgc gacgaggccg ccggcatatt cgaacaggaa
     4501 ttcgccggtg cgcttcttgt attcggcatt caccttctcg gcgttggcgt ccatgtcgtg
     4561 gttccagtgc gccacgcctt gcaaaccttc cgcgagtttt ccgacgttct tgtagaagtc
     4621 cggaatgacg aagccgccgg aaccgccgtt gatcgcgatg ttgaggccga cctgcttgac
     4681 ggtccgcacg atcagaatga ggtcgttcag gtaggacacc gagaacagcg cgttggcgcc
     4741 tgatgacttg accttgttga tcagcgggct ggcatcggtg aacccggccg aatacggttc
     4801 gaacaacacg atctcgacgc cttcggcggt cgcctgttcg cgcagaccgt tggatgtcga
     4861 ggtgccgaac gcggtgttct cgaacaccac cgcgaccttc ggcttgtcgc tgaccagccg
     4921 cgacattcgc aactgcgcca gggcgaattg cgaggcgcgg gcgaacggcg tgaaggtata
     4981 ggtacggccc ttgttgagct ggtcggaact ggatccggtc agcagcggca ctttcgcgcg
     5041 ttccgccact tcgcttgcga tcagcgtcag ggcgctggcg aaacagccgt ggatcgcgga
     5101 cagtttgttg ccggtgatca ggcggtcggt ctccgtgcgc gttaccgtgg tgtcgctctg
     5161 cacgtcggag aggatgagat tgagttttgc accgccgagc gatttgatgc cgccggcgtc
     5221 gttgaccatc tcggccgcga gcttggcggc agcgacgcag cccacgccga tttgcgccat
     5281 gcttccggta gtcgggtaaa gcgcgccgat attgaccgga tcggccgccc agccgcgcat
     5341 cggcaccgcg ccgaacgcgc ctgcggcgag tatgccgccc gtcttcagga gaaactcgcg
     5401 gcggccttgc cgtttcgata aggctcgact taggtcggct ttattgctga cggaatgctg
     5461 acgatctttt ttcatgcggt ttccccctgc gcgattccgg tacaggctgc tgtccgctcg
     5521 ctttcgggca tggtagcgtc cctgaatatt gtttacaata cagtttccaa tattatcatc
     5581 ataattgaaa actcttgccg gctggtgatg cgccggccgc gccgcacggg tggggcgcat
     5641 cttccaaatc ggggccacga tggtcggaaa accagccaaa cacgcgatcg cgcggacgcc
     5701 caaatcccgg cagatgacga tatcgccggc gcgagaaccg gccgcagcgg tcatcgcaaa
     5761 gagcatcgag gaggacatcg tacttgggcg acgtcagccg agggagcgcc tggtcgagca
     5821 ggacctttgc gatctgttcc agacccatcg cggtgacgtg agactggctc tgttcgagct
     5881 cgaaaaaaag ggcttggtgg agcgcatccc gaatcgcggg gcgctggttc gcggattgac
     5941 cccgctggag gtgaaggaga tctacgcggt tcgtgaagag ctggaagtga tggcggtccg
     6001 gatcatcccg tttccggttg gctcaagtga tatcgagagg ctggaggaat tgcaggtaca
     6061 gcacagcgca gccgttgcgg caggtgattt gctaaccgtg ttttacagca accttagttt
     6121 tcaccaggtc ttgttcggcc tctgcggcaa tgcctgtctg atcgaaacga tcgatctgct
     6181 cgcccagaag gtttacggaa ttcgctccta tgcgaatgca tttcctgaat cacttgatcg
     6241 cgcccgccag gaccacctgg atatgatcaa ggcgcttcgt ggttcgcatc gagacgatct
     6301 gatcgtgctc gcgcggcgcc atctcgagcc ttccccgcaa gcctatataa aggcgtatga
     6361 acggcgcttc ggcaaaaccg atttaacgcc cgcgcattag cggcgcccgc gccgagggtg
     6421 tgctgcccgg cgcggacgat gacgatatgg atcgagctat tcggcgctgc tcaccagctt
     6481 gatgcgtggt tccgcggcct ccatcaggct gcgataggcg gcgagatagt cgagcgccat
     6541 gcggcgcgcg gtgaagcggg cctcaaactg ctttcggatg gcatgtctgc tcaagcggga
     6601 cagccgatcg acggcgccga ccgcgctggc ttcatcctcg acgacgaagc cggtcaagcc
     6661 ctcatcgatg atctcgggaa ccgagccatg gttgtatgca atgacaggcg tcccgcatgc
     6721 catcgcttcg atcatgacga ggccgaacgg ctcgggccaa tcgatcggca gcagcaaacc
     6781 aatcgcggcg ctgaggaagt cggatttttc gtgatcgccg atctcg
//
LOCUS       DASWGM010000716         4730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_90786,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000716 DASWGM010000000
VERSION     DASWGM010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90786"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VGA15_20090"
     CDS             <1..819
                     /locus_tag="VGA15_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9590034.1"
                     /translation="MSVHSEVDPLPEGLAILQIGLVEWDLAKNYGAEIALKADVKETL
                     RVLIPALKAAGGAGLEARARQGVAALKSRNWTARRATLVEQISKNSTRSPIDPDFLAL
                     QVIETMPGNAILVDEGLTSSRQINALRPHRDRYGYHALASGGIGWGLPASVGVSMANP
                     DRPVVCFSGDGSAMYSIQALWTAAHHKLPLTVVIVNNGGYRIIKQRLLAFHGDDHYVG
                     MDFVDPPVDFTGLAKSFGLEAMRITAAKDLKAVLASAFSRPGAKLIEVVVDGTV"
     gene            complement(1015..2403)
                     /locus_tag="VGA15_20095"
     CDS             complement(1015..2403)
                     /locus_tag="VGA15_20095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9590035.1"
                     /translation="MKKEIRLNAFAMNCVAHQSPGLWTHPRDRTSEYNRLPYWIDLAR
                     TLERGRFDGLFLADVLGVYDVFGGSPDAALRNATQTPANDPLLVIPAMAAVTKNLGFG
                     VTSNLSYEPPYTFARRMSTLDHLTEGRIGWNVVTGYLDSAARGAGKEKQTAHDDRYDV
                     ADEYMDVVYRLWEGSWEDDAALRDRARGIFADPAKVHRVQHQGNNYRVDAIHLSEPSP
                     QRTPVLYQAGTSPRGRQFAAQHAECVFMSGPSAKIIAPRVAAIRALAREAGRNPAEIL
                     MFSMMTIILGRTEAEASAKYADYVSHVSPEGALALMSGWTGVDFSTYDLDQEVRHVQN
                     DAGRSAMDNITRGDPDRVWTVREVAQHVGIGGIGPVLVGTPDKVADDIEAWFEQTDVD
                     GLNVPFAISPGDFEDITDMLVPEFVKRGRYKAAYQKGTLREKLFGSGRARLAAPHPAT
                     QYRHHQAAKAAE"
     gene            2602..3984
                     /locus_tag="VGA15_20100"
     CDS             2602..3984
                     /locus_tag="VGA15_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX9590036.1"
                     /translation="MTRADAIDRAHQYLHSGEFLAELDRRVAYRTESQNPERRDALRA
                     YLEEELQPAFSQLDFSTRLVESPTGKGPYLVAEYRENSSAPTVLMYGHGDVVDGMAGE
                     WRDNLDPWRTTTSGNRVYGRGTADNKGQHSINLSALRAVREARSGRLGFNAKFIIETG
                     EEIGSPDLRQVCEQLRDELKADLFLGSDGPRLSADRPTIFLGCRGGIRIHLDVNLRDG
                     SHHSGNWGGVLANPATILASAIAGLVDGKGRLLLDELKPPRISNQIRAALADVKIEPM
                     SDEPALSENWGEERLSAAERLYAWNTLEVLAMSSGNIGKPANAIPGRAQAVLQLRFVV
                     GTKVDKIVDVIRARLHGSGFPMVEVKATQSFDASRTDFDSPWVNWAAESIRQTTGKAP
                     AVLPNFGGSLPNDVFSDCLGLPTIWVPHSYPGCSQHAPDEHILLSVTEEALGIMAGLF
                     WDLGEMPRAL"
     gene            4193..4612
                     /locus_tag="VGA15_20105"
     CDS             4193..4612
                     /locus_tag="VGA15_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590037.1"
                     /translation="MTPAPNAIDDAAIIQAFAGIPALLEQDPALIYRGRTLDCECLLG
                     PVDHPFHVSIRGGSIVDLTPAPVLMRSWRFSYRASLTAWAEYWKPAPRPGWHDLLALT
                     KREEATLEGDLHPFMAHLQYFKDVLALPRLDRSAVPT"
BASE COUNT          838 a         1465 c         1532 g          895 t
ORIGIN      
        1 atgtcggtgc acagcgaggt cgatccgttg cctgagggcc tggcgatcct gcagatcggc
       61 ctggtcgagt gggacctcgc caagaactac ggcgccgaaa tcgcgctcaa ggccgatgtg
      121 aaggaaaccc tgcgggtgct gattccggcg ctgaaggccg ccggcggcgc tgggcttgag
      181 gcccgcgcca ggcagggcgt cgccgcgctc aagtcgagga actggacggc gcggcgcgcg
      241 acgctggtcg agcagatttc gaagaacagc acgcgctcac cgatcgatcc ggattttctc
      301 gcgctgcagg tgatcgagac catgccgggt aatgccatcc tggtcgacga ggggctgaca
      361 tcctcgcgac agatcaacgc gctgcgcccg caccgcgacc gctacggcta tcacgcgctg
      421 gcgtccggcg gcatcggctg gggattgccg gcctccgtcg gcgtcagcat ggcaaatccc
      481 gaccggccgg tggtgtgttt ctccggcgat ggcagcgcga tgtattcgat ccaggcgctg
      541 tggaccgccg cgcatcacaa actgccgttg acggtggtga tcgtcaacaa tggcggctac
      601 cgcatcatca agcagcggtt gctggcgttt cacggcgacg atcactacgt cggcatggat
      661 tttgtcgacc cacccgtgga ttttaccggc ctggcaaaat ccttcgggct ggaggcgatg
      721 cggatcaccg cggcgaaaga tttgaaagcc gtactggcgt cggcattttc gcggcccggc
      781 gcgaaattga tcgaggtcgt ggtcgacgga acggtttgag accgacattg cgatgccaac
      841 gggtcgcgcg aatgcgtgcc cgatgatagg ctccgcgaaa gcaatccaca gctacgcaaa
      901 agcttcgtag ggtgggcaaa ggcgcgctcg cgccgtgccc accttttccg cctgaagatg
      961 gtggtgggca cgctgcgctt tgcccacccc acagcgctcg cgatgccgag cgattcattc
     1021 cgccgccttc gccgcctgat gatggcgata ttgcgtggcc ggatgcggtg cggcgagccg
     1081 cgcgcgcccc gagccgaaca gtttttcgcg cagcgttcct ttttgatagg ccgccttgta
     1141 gcgcccgcgc ttgacgaact ccggcaccag catgtcggtg atatcctcaa agtcgccagg
     1201 cgaaatcgcg aacgggacgt tcaatccgtc gacgtcggtc tgctcgaacc acgcttcgat
     1261 atcgtctgcg accttgtcgg gcgtgccgac cagcaccggc ccgattccgc cgatcccgac
     1321 atgctgggcg acctcgcgca cggtccacac gcgatccgga tcgccgcggg tgatgttgtc
     1381 catcgcgctg cggccggcgt cgttctggac gtggcggact tcctggtcga gatcgtaggt
     1441 cgagaaatcg acgccggtcc agccggacat cagcgcgagc gcgccttcgg ggctgacatg
     1501 gctgacatag tcggcgtatt tggctgatgc ttccgcttcg gtgcggccga gaataatggt
     1561 catcatgctg aacatcagga tttcggccgg gttgcggccg gcttcgcggg ccagcgctcg
     1621 gatcgcggca acacgcggcg cgatgatctt ggccgacggg cccgacatga acacgcattc
     1681 ggcgtgctgg gcggcgaatt gccgtccgcg cggcgaggtg ccggcctgat agagcacggg
     1741 ggtgcgctgc ggcgacggtt cgctcaaatg aatggcgtcg acccgataat tgtttccctg
     1801 gtgctggacc cgatgcactt tggccgggtc ggcgaagatg ccgcgcgcgc gatcgcgcag
     1861 cgccgcatcg tcctcccagc ttccttccca gagcctgtag accacgtcca tatattcgtc
     1921 ggcgacatcg taacggtcgt catgcgcggt ctgcttttcc ttgccggcgc cgcgggcggc
     1981 gctgtcgaga tagcccgtca ccacgttcca gccgatccgc ccctcggtga gatggtcgag
     2041 cgtcgacatc cgccgcgcaa aagtataggg cggctcatag gacaggttgg aggtcacccc
     2101 aaagccgaga ttcttggtga cggcggccat cgccgggatc accagcagcg gatcgttcgc
     2161 aggcgtctgc gtggcgttgc gcaacgccgc gtccggactg ccgccgaaca cgtcatagac
     2221 gccgagcacg tccgcgagaa acagaccgtc gaagcgtccg cgctccagcg tccgcgccag
     2281 atcgatccag tatggcagcc ggttatattc gctggtgcgg tcgcgcggat gggtccacag
     2341 ccccggcgac tgatgcgcga cgcagttcat ggcgaaggca ttgagccgga tttctttttt
     2401 catgatcgta agccttgagg cacggcttgc acggcgagtt atcatccgca gccggtcgag
     2461 cgccgggctg ttggcggaaa ttgttgcatt ccggcaggtt ttagcaagac aaaatctcgg
     2521 cgcaatgccc acttccggcc ggcatcgtat ctcgatacgt tggcagaatt cgaagaggtt
     2581 aacgaccgga aaacgcaaga catgaccaga gccgacgcca tcgaccgcgc gcaccagtat
     2641 cttcattccg gagagtttct cgcagaactc gatcgcaggg ttgcgtaccg gaccgaaagc
     2701 caaaacccgg agcgccgcga cgcgctgcgc gcctacctcg aggaagaact gcagccggca
     2761 ttctcgcagc tcgatttctc gacaagactg gtcgaatctc cgaccggcaa gggcccttat
     2821 cttgtcgcgg agtatcgtga gaattcttcg gcgcccaccg ttctgatgta cggacacggt
     2881 gacgtcgtcg acggcatggc aggagaatgg cgggacaatc tggatccgtg gcgcacgacg
     2941 acgtcaggta atcgcgttta cggccgcggc accgctgaca acaagggaca gcacagcatc
     3001 aatctgtcgg cgcttcgcgc cgtgcgcgag gcacgtagcg gccggctcgg cttcaacgct
     3061 aaatttatca tcgaaaccgg cgaggagatc gggtcgccgg atctgcgcca ggtttgcgag
     3121 cagttgcgcg acgagctcaa ggccgatctg tttctcggct ccgacggacc ccggctttcc
     3181 gcggatcgtc ccacgatatt cctcggctgc cgcggcggaa tacggattca tctcgatgtg
     3241 aacctgcgcg acggttcgca tcattcgggt aactggggcg gcgtccttgc caatcccgca
     3301 acgatcctgg ccagcgcgat cgccggcctg gtcgatggca agggacggct gttgctggac
     3361 gaattgaaac cgccgcgcat ttcgaaccag atccgcgcag cgcttgccga cgtcaagatc
     3421 gagccgatgt ccgatgagcc cgccctgtcg gagaactggg gcgaggagcg cctgtcggcg
     3481 gccgagcggc tttatgcgtg gaacacgctc gaagtgctgg cgatgtcatc cggcaacatc
     3541 gggaagccgg ccaacgccat tccgggacgc gcgcaggccg ttctgcagct tcgctttgtt
     3601 gtcggcacca aggtggataa aatcgtcgac gtgatcaggg cgcgtttgca cggcagcggg
     3661 tttcccatgg tcgaggtgaa ggcgacgcag agctttgacg cttcgcgaac cgatttcgac
     3721 agtccctggg tcaattgggc ggcggagtcg atccggcaga ccaccggcaa agcgcccgcg
     3781 gtcctgccga atttcggtgg ctcgttgccc aacgatgtgt tttccgattg cctgggcttg
     3841 ccgacgatct gggtgccgca ctcctatccc ggctgctccc agcacgcgcc cgacgaacac
     3901 attctgctgt cggtcaccga ggaagcgctc ggcatcatgg cgggcctgtt ctgggatttg
     3961 ggcgagatgc cgcgcgccct gtagaattcc gtgcgggcga gggcggcgcg acactgggcc
     4021 tgtggcgcca tgcgagacgc ggcaagacga atatgacgca gctgtggcgg ccggtatcct
     4081 ccgaacgtac cttgcgacag gctgacgttc gggccataga tacggccgat cggaagccat
     4141 tccgttcact cgccaatgtc ggcatttgca ggaaaacgcc tgcttgaagc gcatgacgcc
     4201 agctccgaac gcaatcgacg acgctgcgat aattcaggcg tttgccggaa ttcccgcgct
     4261 gctcgagcag gaccctgcgc tgatttatcg cggccgcacg ctcgactgcg aatgtctgct
     4321 cgggccggtc gatcacccgt ttcatgtgtc gatccgtggc ggcagcatcg tggatttgac
     4381 gccggcgccg gtcctgatgc ggtcctggcg tttttcatat cgcgcgtcgc taacggcctg
     4441 ggcggagtat tggaaacccg cgccccggcc gggctggcac gatctgctcg ccctgaccaa
     4501 gcgtgaggaa gcaacgctgg agggcgacct gcatccgttc atggcgcatc tgcagtattt
     4561 caaggatgtg ctggcgcttc cccggctcga tcgttccgcg gtgccgacat gaccggctat
     4621 atcgagccga tggtcggccg ctatatccat gtcgagatcg atcgcaagat gcatcgcatc
     4681 tatttcgagg aaagcggcgc gggcataccg ctggtgtgtc tgcacaccgc
//
LOCUS       DASWGM010000717         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_479383,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000717 DASWGM010000000
VERSION     DASWGM010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479383"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VGA15_20110"
     CDS             <1..223
                     /locus_tag="VGA15_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9590038.1"
                     /translation="QQSNFHDYRMLRIDQAPKIEVHVIKSGEPPGGIGETGSTAGPPA
                     LRNAILAATGVALRRLPIDRALIAVGKKA"
     gene            220..1566
                     /locus_tag="VGA15_20115"
     CDS             220..1566
                     /locus_tag="VGA15_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9590039.1"
                     /translation="MSPAARRILLSVAAIVVVALLVGLWIIRGPGPLAFAGGPNVALA
                     DYHEANPTGVPAALAQASPVERGAYLARAADCMVCHTTPGGKEYAGGLGFKLPFGTLY
                     STNITPDKETGIGNYSDQDFLNAVHRGIREDGARLYPAMPFTSYTYMTDADALAIKAY
                     LFSLPPVRAPDPDNTLAFPFNQRWAMGFWSALFNPDTRFEPDTSKSPQWNRGAYLAEA
                     LAHCGECHTPRNLAFALDNRQKFAGALTAGWRAYNITSDKTTGIGGWRDDDLVAYLTT
                     GHAAGHGSASGPMGEAVDHSFGQFAPEDIRSLVAYLRSVPPIASPDLPATLAPPAPPS
                     HRDGGGTADARGKMVFEGACASCHGWTGESPISPFATLTGAWAVNDPGATNVAQIVIS
                     GTKRHSEGAISMPAFGNAYSDVEIAAVANYVTARFGSKGSTLTAQDVAELRKQTSQ"
     gene            complement(1607..2101)
                     /locus_tag="VGA15_20120"
     CDS             complement(1607..2101)
                     /locus_tag="VGA15_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9590040.1"
                     /translation="MPKHDLDTIDCRIVAELQSDGRLSNVELADKVGLSPSPCLRRVK
                     RLERDGYIEGYRAALRRDRVGLGFSVFIGVKIDGHANERATAFEKAVVEMPEVIACHL
                     VSGESDYFLEVVVPDLGDYQRFLVGELLNLPIVREVRSNIAIQTLKAGAPLPLAHLEL
                     GLGK"
     gene            2200..2631
                     /locus_tag="VGA15_20125"
     CDS             2200..2631
                     /locus_tag="VGA15_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9590041.1"
                     /translation="MAGISNPRSESAMPDRSTTHPLFDAVERYFDLMFDNDVSRFDQV
                     FASSAQLHGLRDGKLRLLAAQEYSNALASRPSPKSGGAPRQQEILLVDFASPTQALVK
                     VRVRIDTTLYLDYLAYHCIDGAWLITAKSFHIERRYEVAPT"
     gene            complement(2628..>3027)
                     /locus_tag="VGA15_20130"
     CDS             complement(2628..>3027)
                     /locus_tag="VGA15_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HEX9590042.1"
                     /translation="AAADAIVVDFHCEATSEKQGVGFFCDGRASLVVGTHTHVPTADH
                     QILSGGTAYMTDAGMTGDYDSIIGMQKEEPLRRFISGIPSGRFEPALGVATLSGVAVE
                     TDDATGLALRIAPVRIGGRLEPTLPGFWTS"
BASE COUNT          556 a          993 c          946 g          532 t
ORIGIN      
        1 ccagcaatcc aatttccatg actaccgcat gctgcgcatc gatcaggcgc cgaagatcga
       61 ggttcacgtc atcaaaagcg gcgagccgcc cggcggtatc ggcgaaaccg gctcgacggc
      121 gggaccgccc gcgttgcgca atgcgattct cgccgcgacc ggcgtcgcgc tgcggcggct
      181 gccgatcgac cgcgccctga tcgccgtggg gaaaaaagca tgagcccggc cgcgcgtcgc
      241 attctcttga gcgtcgccgc catcgtggtg gttgcgctgt tggtggggct ttggattatc
      301 cgcggccccg gtccgcttgc cttcgccggc ggccccaacg tggcgctcgc ggactatcac
      361 gaggccaatc cgaccggcgt tccggcagca ttggcgcaag caagcccggt ggagcgcggc
      421 gcctatctgg cgcgggcggc ggactgcatg gtctgccaca ccacgccagg cggcaaggaa
      481 tatgccggcg gtctcggatt caagctgccg ttcggcacgc tttattcaac caacatcacg
      541 ccggacaagg agaccggcat cggcaattac agcgatcagg atttcctcaa cgctgtccat
      601 cgcggcatcc gcgaagacgg tgcgcggctt tatccggcga tgccgtttac gtcctacacc
      661 tatatgaccg acgccgacgc tttggcgatc aaggcctatc tgttcagcct gccgccggtg
      721 cgcgcgcctg accccgacaa tacgctggcg tttccgttca accagcgctg ggcgatgggc
      781 ttctggtcgg cgctgttcaa tccggatacg cggtttgagc ccgatacctc gaagagtccg
      841 caatggaacc ggggcgccta tctcgcggag gcgctggcgc attgcggcga atgccacacg
      901 ccgcgaaacc tcgccttcgc gctcgacaac cgccaaaaat tcgccggcgc gctgacggcg
      961 ggctggcgcg cctacaacat cacttcggac aagaccaccg gcataggcgg ctggcgcgac
     1021 gacgatctcg tcgcctacct cacgaccgga catgcggcag gccacggctc cgcgtcgggg
     1081 ccgatgggcg aagcggtgga ccacagcttt ggccagtttg cgccggaaga catccgcagc
     1141 cttgtggcct atctgcgcag cgtgcctccg attgcctcac ccgatctgcc ggcgacgctg
     1201 gcgccgccgg cacctccgtc gcaccgcgat ggcggcggca cggcggacgc ccgcggcaag
     1261 atggtgttcg aaggcgcctg cgccagttgc cacggctgga ccggcgagag tccgatctcg
     1321 ccgttcgcga cgctgaccgg ggcgtgggcg gtcaacgatc ccggcgcgac caatgtcgcc
     1381 cagatcgtga tttccgggac gaagcggcat agcgaaggcg ccatctcgat gccggcgttc
     1441 ggcaacgcct attccgatgt cgagatcgcg gccgtcgcca actacgtcac cgcacgtttc
     1501 ggcagcaaag gctcgacgct caccgcgcag gacgtggcgg agttgcgcaa gcagacctcg
     1561 cagtaaccga cgtcatcggc ggcggcatga tcgctgcgag gcttcgctat ttgccaaggc
     1621 caagttcgag gtgagcgagc ggcaatggcg ccccggcctt gagcgtctgg atcgcgatgt
     1681 tgctgcgtac ttcgcgcacg attggcaaat tgagaagttc gccgaccaga aagcgctggt
     1741 agtcaccgag atcaggtacg acgacttcga ggaaatagtc cgattcgccg gagacgaggt
     1801 ggcacgcaat gacctcaggc atctcgacga cggccttctc gaatgccgtc gcgcgctcgt
     1861 tggcgtgccc atcgatcttg acgccgatga agaccgagaa gccaaggccg acgcggtcgc
     1921 gccgcaacgc cgcccgatag ccttcgatgt acccatcccg ctccaggcgc ttgacgcggc
     1981 gcaggcacgg cgatggcgac aagccgacct tgtcggccaa ttcgacgttg ctcaaacgtc
     2041 catcggattg cagctcggcg acaatgcggc agtcgatggt atccagatca tgttttggca
     2101 tactgacgca tcctgagaac caaaaagggc agattgccgc tctttcctgc ccagtaatag
     2161 cgcagtttgc aaggacatgc ccgcgggccg catgtcacgg tggccggtat cagcaacccc
     2221 aggagtgaat cagcaatgcc cgatcgttcg accactcatc ccctgtttga cgccgttgag
     2281 cgctatttcg acctgatgtt cgacaatgac gtgtcgcgct tcgatcaggt gttcgcgtcg
     2341 agcgcacaat tgcacggact gcgagacgga aaattgcgcc tgctggccgc gcaggaatac
     2401 agcaatgcgc ttgcctccag accgtcgccg aagtctgggg gcgcgccgcg ccagcaggag
     2461 atattgctgg tcgatttcgc ttcacccacg caggccttgg tgaaggttcg ggtcaggatc
     2521 gacacgaccc tgtatctcga ctacctcgcg taccactgca tcgatggcgc atggctgatc
     2581 accgccaaat cttttcatat cgagcggaga tacgaggtag ctccaaccta actcgtccaa
     2641 aatcccggca gcgtcggctc cagcctgccg ccgatccgga ccggtgcgat ccgcagcgcc
     2701 aggccggttg catcgtcggt ctcgaccgca acgccgctga gcgttgcgac gccaagcgcc
     2761 ggctcgaatc ggcccgacgg aatgcccgat atgaaacgcc gcagcggctc ttccttctgc
     2821 atgccgatga tcgagtcata atcgccggtc atgccggcat cggtcatgta ggcggtgccg
     2881 ccggaaagga tctgatggtc ggcggtcggc acatgggtgt gggtgccgac gacgaggctg
     2941 gcgcggccgt cgcagaaaaa tcccacgccc tgcttttcgc tggtcgcctc gcaatggaaa
     3001 tccaccacga tggcatccgc cgccgcg
//
LOCUS       DASWGM010000718         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_197254,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000718 DASWGM010000000
VERSION     DASWGM010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197254"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..876
                     /locus_tag="VGA15_20135"
     CDS             211..876
                     /locus_tag="VGA15_20135"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9590043.1"
                     /translation="MVPSDSKPDVVIVGAGIAGSALAISLAGSGHSVTLLEKSTVHVD
                     RVRGEFIVPWGVAEAKTLGILDVLESAGGNYTVRSIPYGEELSPEDARKYSVPMDQMV
                     PGVRGAMNLGHPRICNALNAAAMAAGATLLRGVDRIQVNAGLPPQIEFTHSGLNYTLS
                     PRIVIGADGRGSTVARQVGFERLADPIHHILSGLLVDDVSAWPLDEQSVGVDGDVGFY
                     VFP"
     gene            1187..1393
                     /locus_tag="VGA15_20140"
     CDS             1187..1393
                     /locus_tag="VGA15_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590044.1"
                     /translation="MRRLRFSSRPLSTYRMEFTDEARRRRRMGRRRMVADPELTLWFM
                     ALQKGPFAVPSEAFSERIWYRLLD"
     gene            complement(1390..>1925)
                     /locus_tag="VGA15_20145"
     CDS             complement(1390..>1925)
                     /locus_tag="VGA15_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX9590045.1"
                     /translation="IGSFSKSIAPALRVGYIVAPWAMLSRMLALKTDAGSGALEQMVL
                     AEYCAPHFASHVPKLTRGLRAKLDTLMEALNEQFGTTAEFEDPKGGIFLWVKLPDHVD
                     TLKLYQSALAAGVAINPGPEWSTDKAYAGCRLRLCFASPSHEQIREGVAVLAEVCAKE
                     FGVPARSANVEKHVRAT"
BASE COUNT          349 a          586 c          601 g          389 t
ORIGIN      
        1 tcgacatagc gggtaaaacg caattcaatc tctttcgctc cgtggagacc catcccagcc
       61 cccttgtttc agtgggattc gcctccataa atgattctca aatacccgaa actgcggctt
      121 tctgactcag tagtactagc gtggtcgccg ctatcatgcc gtatcaatcg ccgttcgcgg
      181 tgcggtgaaa tatcggcggg cgcaaaacca atggtacctt cggatagcaa gcctgacgtc
      241 gtcatcgtgg gagcgggcat agcgggcagc gcgctcgcga tttcgcttgc cggttccggc
      301 cacagcgtca ctctgctgga gaagtcgacg gtgcatgtcg atcgtgttcg cggcgaattc
      361 atcgtgccgt ggggcgtggc ggaagccaaa acgctcggga ttctggatgt cctggagagc
      421 gccggcggaa actacaccgt ccggtcgatt ccatacggcg aggagctttc gccggaagac
      481 gcccgaaaat attcggtgcc gatggatcag atggttccgg gagtccgagg cgccatgaat
      541 ctggggcatc ccagaatttg caacgccctc aatgctgccg cgatggctgc cggtgccacg
      601 ctgcttcgcg gcgtcgatcg cattcaggtc aacgccgggc ttcctcctca aattgaattc
      661 acgcactccg gcttgaacta caccctctca cctagaatcg tcattggagc cgatggccgc
      721 ggctctacgg ttgcccgtca ggtcgggttc gaacggcttg ccgacccgat ccatcacatc
      781 ctgagcgggc ttctggtcga tgacgtcagc gcgtggccgc tcgacgaaca gtcggtcggc
      841 gtcgatggtg acgttggatt ttatgttttt ccttaaggta acggaagagt ccggctttac
      901 gccactcacg gtctcgatca acgcaaccgc ttcgcgggcg atggcgctac cgaaagattc
      961 ctgcaggcct ttgaccggcc gtcgattccg cacggcggct cgctttcgcg aggtcggcca
     1021 tccggtccct gccatggcta tccaaacaat gacgtctgga tcgacaaccc cgtggctccc
     1081 ggtgtcgtcc tgatcgggga cgcggcaggc cacaacgacc cgtcgggcgg gcagggcctg
     1141 gaccccggaa acgttcgctt cttacgcgtc gcagcggcgc gagcaaatgc ggcggcttcg
     1201 gttcagctca aggccgcttt cgacctatcg gatggaattc accgacgagg cgaggcgacg
     1261 ccgacggatg gggcgcaggc gcatggtggc cgatccggaa ctgacgcttt ggttcatggc
     1321 gctgcagaag ggaccttttg ccgtcccgag cgaggcattt tcggaacgga tatggtaccg
     1381 cttgctcgat taggtcgcgc gcacgtgctt ctccacattt gcactccggg ccggcacgcc
     1441 gaattcctta gcgcacactt cggccagcac ggcgacgccc tcgcggatct gttcatggga
     1501 ggggctcgca aaacacagcc gcaaacggca gccggcatag gccttgtcgg tggaccattc
     1561 cggcccgggg ttgatggcga cgccggcggc aagtgctgac tggtagagtt ttagcgtgtc
     1621 gacatggtca gggagtttca cccacaggaa gatgccgcct ttggggtctt cgaattccgc
     1681 ggtcgttcca aactgctcat tgagcgcctc catcagcgtg tcgagcttgg cgcgcaggcc
     1741 gcgcgtcagc ttcgggacgt ggctggcaaa atgcggggcg cagtattcgg cgagcaccat
     1801 ctgctccagc gcgccggagc cggcgtcggt cttcagcgcc agcatccgcg acagcatggc
     1861 ccagggtgcg acgatgtagc cgacccgcaa cgccggcgcg atcgatttcg agaatgagcc
     1921 gatgt
//
LOCUS       DASWGM010000719        11722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_618797,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000719 DASWGM010000000
VERSION     DASWGM010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618797"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VGA15_20150"
     CDS             <1..542
                     /locus_tag="VGA15_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590046.1"
                     /translation="RNHALFACITASPIAVRVLRPALQTGELAMITAEKCRMRAAECR
                     VLASSIDIPAQRSLEQSIMALNWEALADLIDHDNSQVTATVFSVSNLSEKDAPKGCGN
                     MPKYFFMVRHGDQTSHQSDGIEFPDLGAVQLEATKSAGEILRDLSHPIEAGSEWRMEV
                     VDEARKPLFSLRVIAELHE"
     gene            complement(841..1071)
                     /locus_tag="VGA15_20155"
     CDS             complement(841..1071)
                     /locus_tag="VGA15_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590047.1"
                     /translation="MSRLPFIFLFLSMGLCSASAQGGHLGTPQEQKACSRDASHFCRK
                     QLGDDAAVQQCLQQNRAKLSAACKKVFEGHGM"
     gene            complement(1913..3868)
                     /locus_tag="VGA15_20160"
     CDS             complement(1913..3868)
                     /locus_tag="VGA15_20160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX9590048.1"
                     /translation="MAQGMKRLGMPIAALLGVALIGLISTSWFLDRDALRQAVEAQIR
                     AVTGLDLVIKGNIDVSVFPGSYVSFHDVGLKGGGTADPALAVDVLTANLRLLPLLLRR
                     FEIADVMMLRPHIHVTRDAGGGSNWTPFIDTIARAMKPGADNQVSFSEIRIQDGALTY
                     EDAASHLSETLGDIDLSLAWPSISRSFAATGQFDWRGERVDGSISASDFVAALSGDRS
                     GLKARLASAPLKLAFDGTVANRTSTMMEGTLTVDSISLRNALRWAGQAPPGSGGFGRF
                     ALRARANVVGASIALTNVNIELDGNVAEGVLTYVNNGRQTLQATLAAGALDFTPYVST
                     FRLLASGAHDWNRQLFDLNSLSTTDLDMRLSAARVTVNSTKLGRTAFGANLRGGALAL
                     SVGEAQVYGGIAKGSFGIARSDAVADVKAQFSFTDVDLQSCASELFGVSKLSGRGNLN
                     VSLVASGSSPFGLAQSLDGTATLTGHDGAISGFNVEQLLKRLERRPLSGSGNFRTGST
                     PYDNLNIAVKFSEGIATAQDVRVEGPAARITLSGTASVPAREYDMKGVASLNSVPNAP
                     PGFDLPFVVQGPWDDPLIFPDPESLIRRSPASAPLLDAVKDRKTRDAVRSVLERFIGS
                     GSKPAEPDAAAEPAPASAPVETTAKPD"
     gene            4182..4907
                     /locus_tag="VGA15_20165"
     CDS             4182..4907
                     /locus_tag="VGA15_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX9590049.1"
                     /translation="MNKVYPDAKSALDGILKDGMMIMSGGFGLCGIAETLSDALRDSG
                     VKNLTVVSNNAGVDGIGLSRLLETRQIKKMISSYVGENKLFAQQFLAGELELEFAPQG
                     TLAERIRAGGAGIPAFYTKTGVGTLIAEGKEVKEFDGEKYIMERGLFGDLAIVHAWKG
                     DTAGNLVYRKTARNFNPMMATAAKITVAEVEHLVPAGEIDPDQVHTPGVFVKRIIEVG
                     TALKRIEQRTMRKRDAAATGEKV"
     gene            4907..5557
                     /locus_tag="VGA15_20170"
     CDS             4907..5557
                     /locus_tag="VGA15_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEX9590050.1"
                     /translation="MAWTRDQMAARAAKELRDGFYVNLGIGIPTLVSNYIPEGVDVQL
                     QSENGMLGMGPFPYEGEEDPDLINAGKQTVTELPTTSYFSSADSFGMVRGGHIDLSIL
                     GAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHSAKDGPKLLKKCNLP
                     LTGEKVVDMVVTDLAVFTIDKHGKDGMALIELADGVTLDEVKSKTEAEFRVALKNA"
     gene            complement(5737..5898)
                     /locus_tag="VGA15_20175"
     CDS             complement(5737..5898)
                     /locus_tag="VGA15_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590051.1"
                     /translation="MPSGYENLSYTVRIKGSGTKQEFAEVHEAVMATSPNFYNMSRPV
                     ALKPVLVVE"
     gene            complement(5936..6211)
                     /locus_tag="VGA15_20180"
     CDS             complement(5936..6211)
                     /locus_tag="VGA15_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590052.1"
                     /translation="MVAQSKTAKPTVVNGINVVDLFTLIDGVKRTPQKARQTGGSRRP
                     GRARRGAVRKSRVLKSAENGCHAGSRSTSMSPANWEDRTFSPIRRNI"
     gene            complement(6256..7191)
                     /locus_tag="VGA15_20185"
     CDS             complement(6256..7191)
                     /locus_tag="VGA15_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590053.1"
                     /translation="MNAIDKVSDGSERYARCVESSKRVRWDIDKDVIRGRRFDVAHKF
                     LPDALAMADAFTTLSADEKRFVSQIQGRTYANMFGLVERFINAKVLELSHEHWFGDQV
                     ALEALVRFSDEELKHQALFRRIETMVGDVLPDGYCFDVDPNGVAHAVLGKSTWAVLAL
                     TLDIELFSQLHYRQSIDPDPGLSELFKDIFLFHWKEESQHAILDELEWVRHDAGLTPE
                     QRDSAVDEFIELVAAVDGILQAQAKADAGYFAASCGRAVPAAETRAIEAAFLKAYRWQ
                     YIHSGAQHPHFGKVLSSLITETQGQRIQSALATLQ"
     gene            complement(7357..8337)
                     /locus_tag="VGA15_20190"
     CDS             complement(7357..8337)
                     /locus_tag="VGA15_20190"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9590054.1"
                     /translation="MPRDYQVNEYVRIDPSTTCQFLFDVGQIYSIDDCIPYAEFRQTR
                     VAKEWARPLGLVDQLAATLDKSAMSFSVFAIFRNEAEGRVDDEMRRRMRLIVPHVRRA
                     VLIGNVLDLNTTEKTAFADTLNGLAAGVFLVDENARVVSANTSGQAMLEDGEILRQKD
                     FVLTAVNARIGTTLPKIIALARDGDAAVGVNGIALPLSSPPEPWLAHILPLTSGARRD
                     AGIAHSAVAAVFVHKASIETPSAMETMSKLYRLTPGELRVLAALSEVGGIAAVAEVVG
                     ISEATVKTHLQRLFGKTGTNRQTELVKLVAAHASPLRHAPLRTPSPLRTQ"
     gene            complement(8820..10592)
                     /locus_tag="VGA15_20195"
     CDS             complement(8820..10592)
                     /locus_tag="VGA15_20195"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HEX9590055.1"
                     /translation="MKAFSKVLLFSLAAMVSTIDPTFAQPASGRPATPTLQYAKQGWT
                     AEDRQAFYTTSQGSHMIPYLWFKALRRLDVDEPFGGDQLARYGYLPNDTKLNPEGLPV
                     GFVIDGDAKTGFLGMTCAACHTAQIEYQKDGATQQLRIDGAPATANFQLFLTDLEAAA
                     KETIKDTNRLEQFARSVLGTSPSAAKVAALKTDFGAWVKQYSAFMDASLPASSPWGPG
                     RLDAFGMIFNRVAARDLGIDANFKIADAPVSYPFLWNASRQDKTQWNGFVPNGLFIHA
                     LGRNTGEVFGVFADFRPSKRTLPRFDFSNNSVKFAGLQQLEEKIAVLKPPPWPFDLKQ
                     DLVDRGRVLYAQNCGSCHDEKPLPDGTWLTPVNAIGTDPRMFKNADRSSQSGLLVGAL
                     TPNPPGATLAETAKTHDILATTVVGALINEAFPLTFRNRNSGVWQAIRKDLAHLLPES
                     QNTPIVVLLLQPKVRANLKSHVEDRLRTLFVPPAPDAPPAYESRVLHGIWATAPYLHN
                     GSVPNLWELLLPPEQRKPSFMVGSKVFDPVNVGYVTDSSPFKDGKLVVGSGAEPGNSN
                     AGHDFGRNLNDDERWALIEYLKQL"
     gene            complement(10937..>11722)
                     /locus_tag="VGA15_20200"
     CDS             complement(10937..>11722)
                     /locus_tag="VGA15_20200"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9590056.1"
                     /translation="IVWYLRKNLPESPRWLESQGRTAEAEALMQAIEKESAGGGTLPP
                     VVVPAPVPQVSASDMLRPPLLQRMLVGCWVLITINTLIFGFVIFLPQFFLRQGLTIAN
                     SLAYTLVLSVASLVGCAVGAYLSDAIGRRASIIGSSIVTIVSGYIYARFSAASDPAIV
                     LSVGFVLIVAIYVQTAILFGVYTPELFPTEIRLRANGICNTFGRGATVVSPFIVGALM
                     ASYQLPGVVWLMIGLVVVQIVVVWAWGVEPRNRGLEDVSTVTA"
BASE COUNT         2314 a         3514 c         3643 g         2251 t
ORIGIN      
        1 gccgcaacca tgcactgttc gcttgcatta ccgcttcgcc tatcgccgtc agagtattgc
       61 gtcccgcact tcaaaccggg gaactggcta tgatcaccgc agaaaaatgc agaatgcgcg
      121 ctgcagagtg cagagtactc gcgtcgtcga ttgatattcc ggcccaacga tcacttgaac
      181 aatcaatcat ggcgcttaat tgggaagcat tagcagatct gattgatcac gataactctc
      241 aagtcacggc gacggtcttt tctgtatcaa atctctccga gaaggacgcc ccaaagggct
      301 gtggaaatat gccgaaatac ttttttatgg tccgtcacgg tgaccagacg tctcaccaaa
      361 gcgacggaat cgagttcccc gacttgggcg cggttcaact ggaagcgaca aaatccgccg
      421 gcgaaatact ccgtgattta agccatccaa ttgaagctgg ttcggaatgg cggatggagg
      481 ttgtagacga ggcccgtaag ccgcttttca gccttcgcgt tatagccgag cttcacgagt
      541 gaggccgcct cgtcgtgaca gttgccggtt ctgagaaaga gagcccgccg acttgggcgg
      601 gcgaagtttc gggagagcgt cccagacgga acgccgtccc caagctgcac gattcggtct
      661 ttctcgccaa ggttgaattc aagtgcggtg tccaatctag gcggactgtg tcatttgggt
      721 ttttgtcgat atgaacgcaa tgcccgctta tggccaacag tcgactagtt cgtggcgaat
      781 tggcagcgtc gctttgagcc gacgcctagc gcgccgatgg gggtcgacac gaatgacgca
      841 ctacatgcca tggccttcaa aaactttctt gcatgccgca ctcagctttg cgcgattctg
      901 ctgcaagcat tgttgaaccg ctgcatcgtc acccagttgt ttgcggcaaa agtgggaggc
      961 atcgcgcgaa cacgcttttt gctcctgtgg cgtgccgaga tgcccgccct gtgctgatgc
     1021 tgaacatagg cccatcgaca aaaacaaaaa gataaatggt agtctagaca ttctggttgc
     1081 tccgtcaggt tcaaagtcat gcgttttgcg atcaacgtcg caacaccgga acgagcccga
     1141 gctacccaat cctaggcagg gattcttccg gccataggat cagccaccgg ccgcgcgccg
     1201 tccaccagcg gggtttgccg ccagccgagc cagtgctggc gcggcgatga cggttccaaa
     1261 agcgaaaaat ggtggcgaga attgaggttc gcttgagcca tcctcctatc gtttccccaa
     1321 ccgcagtaac gcacgaactg ccgaaggttt cccgaagcgg tgttctattt catccgggat
     1381 catgaccgtt ccccggtggg gcttgcgcca tcataccttg aatttgcgcc gccgatccgg
     1441 agggtctcaa tcggcagagc aggggcaatg gcaagacgat tcagttctgt ggcgacggct
     1501 ggcgcagccg atcggacgtg gcgccgatgc cagcgattcc gatcatctcg cggaataaaa
     1561 atccgcaccg gccgatacag caattcaaag agggtgtgac catgaaggcg tcggtgggca
     1621 gaattgtgca ttctgatagc gaagcgatcg cggacagggg tccggcacgg ccgccccgag
     1681 attgagagat cggccgcaac ccccggcgcg gccgtcatgc ggtctgtttt tcctccaaga
     1741 ttgccggacc gcagggagga gcccgcggcg cttaaaaaac gccgcgggct tttttgtttc
     1801 tccgtcacgt gcgcggcggc ggccgaagtg ctgaaacact tcgaatcacg gggcagagtt
     1861 caatcaggcc cgcctgacgg gtgcagattg cgctgaaggg cccgtcgaag cgtcagtccg
     1921 gctttgctgt cgtctcgacc ggcgcgctgg cgggcgctgg ctcagcggcg gcatccggct
     1981 ccgccggttt tgagccgctg ccgatgaagc gctccagcac cgagcggacc gcgtcgcggg
     2041 ttttgcgatc cttgaccgcg tcgagcaagg gtgcggaggc cggcgagcgc cggatcaggc
     2101 tttccggatc cggaaaaatc aagggatcgt cccacggacc ctgcaccacg aacggcagat
     2161 cgaagccggg cggcgcgttc ggaaccgaat tcaggctggc gacccccttc atatcgtatt
     2221 cgcgcgccgg caccgacgcg gtgccgctca aggtgatgcg cgccgccggt ccttcgacgc
     2281 ggacatcctg ggcggtggcg atcccttcgg aaaatttcac ggcgatgttc agattgtcat
     2341 agggcgtcga gccggtgcga aagttgccgc tgccggacag cggccggcgc tccagccgct
     2401 tcaacaattg ctcgacgttg aagccggaga tcgcgccatc atgaccggtc agcgtggcgg
     2461 tgccatcgag cgactgcgcg aggccaaacg ggcttgagcc cgacgccacc agcgagacgt
     2521 tgaggttgcc gcggccggac aatttgctga caccgaacaa ttcggatgcg caggattgca
     2581 gatcgacgtc ggtgaacgag aactgtgcct tgacgtcggc gaccgcgtcg gaacgcgcga
     2641 tcccgaacga gcccttggcg ataccgccat acacctgcgc ctcgccgaca ctgagcgcca
     2701 gcgcgccgcc gcgcagattg gcgccgaacg cggtgcggcc cagtttggtc gaattgaccg
     2761 tgacccttgc cgccgacaga cgcatgtcga gatcggtagt ggacagcgag ttcagatcga
     2821 acagctgccg gttccagtca tgcgcgccac tggcgagcag gcggaaggtg gagacatagg
     2881 gggtgaagtc gagcgcgccg gcggcgagtg tcgcctgcag cgtctggcgg ccattgttga
     2941 cataggtcaa tacgccctcg gcgacgttgc catcgagctc gatgttgacg ttggtcagcg
     3001 cgatcgacgc accgaccaca ttggcgcgcg ctctcagcgc gaagcggccg aacccgccgc
     3061 tgccgggcgg cgcctgaccg gcccagcgca acgcgttgcg caacgatatg ctgtcgacgg
     3121 tcagcgtgcc ttccatcatc gtggaggtgc ggttggcgac ggtgccgtcg aacgccagtt
     3181 tcagcggcgc gctggcgagc cgggccttga ggccggatcg gtcgccggaa agggctgcaa
     3241 caaagtcgct ggcgctgatc gagccatcga cgcgttcgcc gcgccagtcg aattgcccgg
     3301 tcgcggcgaa ggaacgcgag atcgacggcc atgccagcga aaggtcgata tctccgagcg
     3361 tttcggacag gtggctggcg gcatcctcat aggtcagcgc gccgtcctgg attctgattt
     3421 cagagaacga cacctggtta tcggcgccgg gcttcatggc gcgcgcgatg gtgtcgatga
     3481 acggcgtcca gttgctgccg ccacccgcgt cgcgggtgac atggatatgg ggacgcagca
     3541 tcatcacgtc ggcgatctcg aagcgccgca gcagcagcgg cagcaagcgc aaattcgcgg
     3601 tcaggacgtc gacggcgagc gcagggtcgg cggtgccgcc gcccttcagc ccgacgtcat
     3661 gaaaggagac gtagcttccg ggaaacaccg aaacgtcgat attgcctttg atcaccagat
     3721 cgaggccggt cacggcgcgg atttgcgcct cgaccgcctg gcgcaaggcg tcccggtcga
     3781 gaaaccagga cgtcgagatc aggccgatca gcgccacgcc gagaagcgcg gcaatcggca
     3841 tccccaggcg cttcattcct tgggccatcg tcaatgacat atccgggtcg ggttggtgtc
     3901 gtcgaatcgc ggttgagtcg gggcgggctg tacatcaacc gaacgcgggt tggcccgatt
     3961 tcggtagata tcacagtccg gatcggcgat agccgacatg caggggcccc acgccaaccg
     4021 gtgcaacttg aggggttttc ttgacacttt caaggccatc cggtgttcca gacccgtgca
     4081 aaagtccaat gattgacgcc ttgcgacgat ttcgcctaat aatcccaaga tacataccct
     4141 cgtgctggcc ataagccgcc ccaccgtcag gtcgcatttt catgaacaag gtctatccgg
     4201 acgccaaatc tgcactcgac ggtatcctca aggacggcat gatgatcatg tccggcggct
     4261 ttggcctgtg cggcatcgcc gagaccttgt ccgacgcgct gcgcgactca ggggtgaaga
     4321 acctcaccgt ggtctccaac aatgccggcg tcgacggcat cggattgagc cggttgctcg
     4381 agacgcggca gatcaagaag atgatctcgt cctatgtcgg cgagaacaag ctgttcgccc
     4441 agcagttcct cgccggcgaa ctggagctgg aattcgcgcc gcagggcacc ctggccgagc
     4501 gcatccgggc cggcggcgcc ggcattccgg cgttctacac caagaccggc gtcggcaccc
     4561 tgatcgccga aggcaaggaa gtgaaggaat tcgacggcga aaaatacatc atggagcgcg
     4621 ggctgttcgg cgacctcgcc atcgttcacg cctggaaggg cgataccgcc ggcaacctcg
     4681 tgtaccgcaa gaccgcgcgc aattttaacc cgatgatggc gacggcggcg aaaatcaccg
     4741 tcgccgaggt cgagcacctg gtgccggccg gcgaaatcga tcccgaccag gtccatacgc
     4801 ccggcgtgtt cgtgaaacgc atcattgaag tgggaaccgc ccttaaacgc atcgagcagc
     4861 gcaccatgcg caagcgcgac gcggccgcaa ccggagagaa agtctgatgg cctggacccg
     4921 cgatcaaatg gctgcccgtg ccgcgaagga attgcgcgac ggattctacg tcaatctcgg
     4981 catcggcatt ccgacgctgg tttcgaacta catccccgaa ggcgtcgacg tccagctgca
     5041 gagcgagaac ggcatgctcg gcatgggacc gtttccgtat gagggggagg aagaccccga
     5101 tctgatcaac gccggcaagc agaccgtcac cgaattgccg accaccagct atttctcgag
     5161 cgccgattcg ttcggcatgg tgcgcggtgg gcacattgat ctatcgatcc tcggcgcgat
     5221 gcaggtggcg cagaacggcg atctcgccaa ctggatgatc ccgggcaaga tggtcaaggg
     5281 catgggcggc gcgatggacc tggtggcggg tgtcaagcgc gtggtggtgg tgatggagca
     5341 ttccgccaag gacggcccaa agctcctgaa aaagtgcaac ctgccgctga ccggcgagaa
     5401 ggtggtcgac atggtggtga cggatctcgc ggtcttcacc atcgacaagc acggcaagga
     5461 cggcatggcg ctgatcgaac tcgccgacgg cgtcacgctc gacgaagtga agtcgaagac
     5521 cgaagccgag tttcgggtgg cactgaagaa cgcgtgaatt gattctccat tccgtcatga
     5581 ccggccttcg tcccggccat ccacgtcttt tttcttacct gctccagggt ggggacgccc
     5641 ggcacaatgg acctgtgctt ggaccggcga agccggaccc gagtgccggg catgattagc
     5701 tttgcaaggt tggcaaaggc ggcagtcttg agcccatcac tcgacaacca ggaccggttt
     5761 gagagccacg ggccgggaca tgttgtagaa gttgggcgag gtcgccatga ccgcctcgtg
     5821 gacctcggcg aactcctgct tggtgccgct gcccttgatg cgaacggtgt agctcaggtt
     5881 ttcgtagccc gacggcacgg cgggatcgat cccgaggaag ccgcgcagat cgatctcaga
     5941 tgttcctgcg gattggcgaa aaggttcgat cctcccagtt cgcagggctc atcgatgtcg
     6001 atcgcgaacc ggcgtggcac ccgttctccg ccgatttcaa aactctcgac ttgcgcacgg
     6061 ctccgcgcct ggctctgcca ggtcgtcgtg acccgccagt ttgtcttgcc ttttgcggcg
     6121 tccgcttgac gccgtcgatc agggtgaaca ggtcgacgac attgatgccg ttgacgacgg
     6181 tgggtttggc ggtcttggat tgtgcaacca tgaaaggttt cctttggctc ggtggtggta
     6241 tgggttcatc aagagttatt gcagcgtggc gagcgccgac tggatgcgct ggccctgggt
     6301 ttcggtgatc agactggaca gcaccttgcc gaagtgcggg tgctgcgcgc ctgagtggat
     6361 gtactgccag cggtaggcct tgaggaacgc ggcctcgatc gcccgcgtct ccgccgcggg
     6421 gaccgcgcgg ccgcaactgg ccgcgaaata gcccgcgtcg gctttggcct gggcttgcag
     6481 gatgccgtcc accgcggcca ccagctcgat gaactcgtcg accgcggaat cgcgttgctc
     6541 cggcgtcagc ccggcgtcgt ggcgcaccca ctcgagttcg tcgaggatcg cgtgctggct
     6601 ttcctccttc cagtgaaaga ggaagatatc cttgaacagc tcggagagtc cgggatcggg
     6661 atcgatgctc tggcgataat gcagctggga gaacagctcg atatccagcg tcagcgccaa
     6721 caccgcccag gtgctcttgc cgagcaccgc gtgagcgacg ccgttgggat cgacgtcgaa
     6781 gcaatagccg tcgggcagaa catcgccgac catcgtttcg atgcggcgga acagcgcctg
     6841 gtgctttaac tcttcgtcac tgaaccgaac cagcgcctcg agcgcaacct gatcgccgaa
     6901 ccagtgctcg tggctcaatt ccaggacctt ggcattgatg aagcgctcga ccagcccgaa
     6961 catattggca taggtgcggc cttgaatctg gctcacgaac cgcttttcat cggccgacag
     7021 ggtcgtaaac gcatccgcca tcgccagcgc gtcgggaaga aatttgtggg ccacgtcgaa
     7081 gcggcgcccg cggatcacgt ccttgtcgat gtcccaacga acccgcttgg acgattcaac
     7141 gcagcgcgca taccgttcgc tgccgtcact taccttgtcg atcgcgttca ttttctgtgc
     7201 ctctccttgc aatgattgcc gacggcaggc gccggtcggt taatgcagcg atctgtacgg
     7261 aaagctccgg gtgcgcgcgt caaccggcag gatgagcggg tgccgatttg cggccaggcg
     7321 aggcagatgg cctggagcgg tgcacaacgg caacgctcat tgagttctca gggggcttgg
     7381 agttctcagg ggggcatgtc gcagggggct tgcgtgtgcc gcaacgagtt tgacgagctc
     7441 ggtctgccgg ttggtgccgg tcttcccgaa cagacgctga agatgcgtct tgacggtcgc
     7501 ttccgagata ccgacgactt cggcaaccgc agcaattccg ccaacctcgc tcagcgctgc
     7561 gagcacgcgc agttcgcccg gcgtcagtct gtagagtttc gacattgtct ccatcgcgga
     7621 tggtgtttcg atcgaagcct tgtgaacgaa caccgccgcc acggcggaat gggctattcc
     7681 ggcatcccgc ctcgcccctg aggtgagcgg caggatgtgg gcaagccagg gctccggtgg
     7741 cgaggacaac ggcagcgcga tcccatttac tccgaccgcc gcgtccccat cacgggccaa
     7801 cgcgatgatc tttggcagcg tcgtcccgat ccgcgcattg acggccgtca acacaaaatc
     7861 cttctgacga agaatttcgc cgtcctcgag catggcctgc ccggatgtgt tggccgatac
     7921 gacgcgagcg ttttcatcga cgagaaacac gcccgcagcg aggccattta acgtatccgc
     7981 gaatgccgtt ttctccgtcg tgttgaggtc cagtacgttc ccgatcagca ccgcccggcg
     8041 cacatgcgga acgatgaggc gcattcgccg gcgcatctcg tcatcgacgc ggccctccgc
     8101 ttcatttcga aaaatagcga acaccgaaaa gctcatcgcc gacttatcga gggtggccgc
     8161 gagctgatcc accaagccca gaggtcttgc ccactccttg gcgacacgcg tttgccggaa
     8221 ttcggcatat ggaatacaat catcaatgct gtagatttgc ccgacatcaa acagaaactg
     8281 gcaggtcgtg gagggatcga tcctgacata ttcattaact tggtagtccc gaggcatcga
     8341 acctcgggct ccaacacaca aaccggttac cggtcttgcg cgcagcgttc ttcgaataaa
     8401 gcgatgacgc cgacccgccg acgaattccg ccgaccttct caacacgtcg gaccacaggg
     8461 cccgatcgag tgtcgtgtca tagaccgttc cgatcaggtc tgaaagggtt tctgcttccg
     8521 ggatcatctg gtctaccttc atcgcccttt cggatgaagc cgtgatggca ggcgaccacg
     8581 cgtgctactc tcgagaccga tctcaatccg atacgactga ctttcgggcg cactctgggg
     8641 gaaagcccgg gtgcacgcat caaccgatcg gttgagaggc gccgaatagc gcgctggcgg
     8701 tcccggaccg ttgcacaacc gcaacgatca ttgatctctc agggaacttg tctcaggggg
     8761 cttgtgtgtg cggcgaccag tttgacaagg tcggcttgcc gactggtaaa tcctgtcctt
     8821 cagagttgct tgagatattc gatcagcgcc cagcgctcgt catcgttgag gtttctgccg
     8881 aaatcgtgcc cggcgttgct gttgccgggt tcggctcccg agcccaccac gagcttgccg
     8941 tccttgaacg gcgagctgtc agttacgtaa ccgacattta ctggatcaaa caccttgctg
     9001 ccgaccatga aggacggctt cctttgttct ggcggcaaca gcaattccca gaggttcggc
     9061 accgatccgt tgtgaagata gggcgcggtc gcccagatgc cgtgcaacac acgcgattca
     9121 taggcaggag gcgcgtccgg cgccggcggc acgaacaagg ttcgaagccg gtcttcaaca
     9181 tgggacttca ggttggctct caccttgggt tgcagcagaa gcaccacgat cggggtattt
     9241 tgtgactcgg gcaacaggtg tgccagatct tttctgatgg cctgccaaac tccgctgttg
     9301 cgatttcgga atgtcagcgg gaaggcctcg ttgatcaagg ctccaaccac tgttgtcgcg
     9361 aggatgtcgt gagtctttgc ggtctctgcc agcgtcgctc cgggcggatt gggcgtcagc
     9421 gcccccacca gcaagccgga ttgcgacgac cggtccgcgt ttttgaacat cctgggatcg
     9481 gtcccgatcg cattgaccgg cgtgagccat gtgccgtcag gcaagggctt ctcgtcatga
     9541 cagctgccgc aattttgcgc gtagagcacg cggcccctgt ccaccaggtc ttgcttgaga
     9601 tcgaatggcc agggcggcgg tttcaagaca gcgatctttt cttcgagctg ctgcagaccg
     9661 gcgaacttca ccgagttgtt gctgaagtcg aaccgcggca acgtacgttt gctcggtctg
     9721 aaatcagcga atacgccgaa cacctcaccg gtgtttcgcc cgagcgcgtg gataaacaga
     9781 ccgttgggaa cgaagccgtt ccattgcgtc ttgtcctgcc gcgacgcgtt ccacaggaag
     9841 ggatagctga ccggtgcgtc cgcaatcttg aaattggcat cgataccgag gtctctcgcc
     9901 gcgacgcgat tgaagatcat gccgaaggcg tcgaggcgcc cgggccccca cggagatgat
     9961 gccggcagac ttgcatccat gaaggcgctg tattgtttga cccaggcgcc gaagtcggtc
    10021 ttgagggcgg cgaccttcgc ggccgatgga ctcgtgccca gaacggaacg ggcgaattgc
    10081 tcaaggcggt tggtatcctt gatcgtctcc ttggcggcgg cctcgaggtc ggtcaggaaa
    10141 agctggaaat tcgccgtcgc cggcgcgcca tcgatgcgaa gctgttgcgt ggccccgtcc
    10201 ttttggtact cgatctgcgc ggtatggcaa gccgcgcagg tcatgccgag gaagccggtc
    10261 ttggcgtcgc cgtcgatcac gaaaccgacc ggcaatcctt ccgggttcag tttggtatcg
    10321 ttcggcagat agccgtagcg cgccagttga tcgcccccga acggctcatc cacgtcaaga
    10381 cgccgcaacg ccttgaacca gagatagggg atcatgtgcg atccctggct tgtcgtgtaa
    10441 aaagcctgcc ggtcctcggc ggtccagccc tgcttggcgt attgcagcgt cggagttgcg
    10501 ggtctgccgg aagccggctg ggcgaatgtc ggatcgatgg tcgagaccat cgccgcgagc
    10561 gaaaacagca ggaccttgga aaatgctttc atgacaatgt ctcgaatgat ccgaaaggac
    10621 agagcttcgc gcgccgcggc ctatattaag ggatcgcctg ttgctaccgc aagttgtatc
    10681 gcgttaaaat tatttttccg gctaaaaaat aaggtgtcac gcggggattt tgatcatctc
    10741 aattcccgat cgtttttgca agatggtgca ttttgccgat cacgcttttc acaagcgaaa
    10801 gcattgcagg aagggcaaag gcgcgcttgg cgccgtgcca ccactcatgt cgtcatgacc
    10861 aaggatggtg gacacgcgaa gtttaccatc gggcgcgcat tcgcgcgccc accatcagca
    10921 ggtgggcgtg gcgccatcag gctgtaacgg ttgagacatc ctccagcccg cgattgcgcg
    10981 gctccacgcc ccaggcccac accaccacga tctgaacgac cacaaggccg atcatcagcc
    11041 agaccacgcc cggcagctgg taggaggcca tcagggcgcc gacgatgaag ggcgagacca
    11101 cggtcgcgcc ccgaccgaac gtgttgcaga tgccgttggc gcgcagcctg atctcggtcg
    11161 ggaacagttc cggcgtatag acgccgaaca ggatcgcggt ctggacatag atcgcgacga
    11221 tcaatacgaa tccgacgctc agcacgatgg ccggatcgga cgccgcgctg aagcgggcat
    11281 agatgtagcc ggagacgatg gtcacgatcg acgagccgat gatgctcgcg cgccgtccga
    11341 tcgcgtcgga cagataggcg ccgaccgcgc agcccaccag cgatgcgacc gacagtacca
    11401 gcgtataggc cagcgaattg gcgatggtca gcccctggcg caggaagaac tgcggcagga
    11461 agatcacgaa gccgaaaatc agcgtattga tggtgatcag cacccagcag ccgaccagca
    11521 tgcgttgcag cagcggcggc ctcagcatgt cggacgcgct gacctgcggc acgggggccg
    11581 gcaccaccac cggcggcagc gtcccgccgc cggcggattc cttttcgatg gcctgcatca
    11641 gggcttcggc ttccgcggtg cggccctgcg attcaagcca gcgcggcgac tccggaagat
    11701 tcttgcgcag ataccagacg at
//
LOCUS       DASWGM010000720         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2691363,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000720 DASWGM010000000
VERSION     DASWGM010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691363"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..470)
                     /locus_tag="VGA15_20205"
     CDS             complement(168..470)
                     /locus_tag="VGA15_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590057.1"
                     /translation="MTEKPAPGATNEVFTPVRDLPSAPIIFFEFCPTFGNHSGLINIM
                     LAAGIVMLSGGPGTDVVPVAVAHLRCTATAAIQLRDAIDKALLIGTPVQNPEGKAN"
     gene            956..1627
                     /locus_tag="VGA15_20210"
     CDS             956..1627
                     /locus_tag="VGA15_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9590058.1"
                     /translation="MRLLVVEDDPDLNRQLTTALTDAGYVVDRAFDGEEGHYLGDSEP
                     YDAVVLDIGLPKMDGISVLEAWRRNGRAMPVLILTARDRWSDKVQGFDAGADDYVAKP
                     FHLEEILARIRALLRRSTGHAQSELTCGPVSLDTRTGRVSVSGNPIKMTSHEYRLLAY
                     LMHHTGRVVSRTELVEHLYDQDFDRDSNTIEVFVGRIRKKLDVDIIQTVRGLGYLLTP
                     PSPRS"
     gene            1718..>2082
                     /locus_tag="VGA15_20215"
     CDS             1718..>2082
                     /locus_tag="VGA15_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX9590059.1"
                     /translation="MRGSSLATRLFLSATAWVVVILLITGVVLSSVYRDATERAFDRR
                     LNLYLRTLIAEVATPDEPPDRQFQSLGEPLFELPLSGWYWQITRTDSEKAEVRASRSL
                     WDKKLPKLEDHGADLTAAG"
BASE COUNT          384 a          622 c          659 g          417 t
ORIGIN      
        1 gcttctgagc gccgttttgc acgacaagcc cgaattaaaa cgggatgaag cctgatagga
       61 cgaatttcgc cgcgcttgag ccggcatcgc cgacgtcagt tgggtccggt catcctcgcc
      121 gtcgatccgc ggtcgacacg catacgggat ttcaatcgtc gccatcccta gttcgccttg
      181 ccctcgggat tttgaaccgg cgtgccgatc agcagtgcct tgtcgatggc atcgcgcaat
      241 tgaatggcgg cggtcgcggt gcaacgcaaa tgggccaccg cgacgggaac gacatcggtg
      301 cctggtcccc ccgacagcat gacgatgccg gcggcgagca tgatgttgat caggccgctg
      361 tggtttccga acgtcgggca gaattcgaag aaaataatgg gagcggaggg tagatcccgg
      421 accggggtga aaacctcgtt ggtggcgccc ggtgccggct tctctgtcat gatggttcct
      481 tcgccagctt catcataaat tggaatttac ttgataaatt ggaatttacc cagacggcgt
      541 gacgctgccg gcgtggatca agggcatgct ccacaatcgg gcttgagaca agacggcgcg
      601 aatccggaac ctgtccggga tcctgcgttg ctttcgatac tcgcgtcgcc aggcgttcgt
      661 gacatcaacg aatcgagggc gccggggcaa cgtcacagcc gagggttgga acgcgtggcg
      721 cgcgggggga gggccccgtc ctgttggagc tttcacgttg gcatgttagg cgcttggatc
      781 agcggccggc cgctcaaaga ccggtaccgg ccggccttgg acgcgaccgg aaaatttggc
      841 aaaagcggtt atgaagaaaa agtaatattc ggggttattc ttgttcatag gccattcacg
      901 cccgcgaggc tcatcttgag ggcggcggtt ctgccattca aatgcggaga ctactttgcg
      961 cctgctggtt gttgaagacg atcccgatct caatcgtcag ctcacgacgg cgctgacaga
     1021 cgccggctac gtggtcgatc gcgccttcga cggcgaggag gggcattatc tcggcgacag
     1081 cgagccttac gatgccgtcg tgctcgacat cggcctgccg aagatggacg gcatctcggt
     1141 gctggaggcc tggcggcgca acggacgcgc gatgccggtg ttgatcctca ccgcccgcga
     1201 tcgctggagc gataaggtgc agggcttcga cgccggcgcc gacgactacg tcgcaaaacc
     1261 gtttcatctc gaggaaatcc tggcgcggat ccgggcgctg ctgcgccgct caaccggtca
     1321 cgcccagtcc gagctgacct gcggcccggt gtcgctggat acccgcacgg gccgggtcag
     1381 cgtgtcaggc aatccgatca agatgacgtc gcacgagtac cggctgttgg cctatctcat
     1441 gcaccacacc gggcgcgtgg tgtcgcgcac cgaactcgtc gaacatctct acgatcagga
     1501 tttcgaccgg gactccaata ccattgaagt gttcgtcggc cgcatccgca agaagctgga
     1561 cgtcgatatt atccagaccg tgcgggggct cggctatctg ctgacgccgc cgtcgcctcg
     1621 gtcttgatta aaggtgttga tcaggcgcca gacttaacgt tttgtttgag catgattttg
     1681 tccgaagatc ggcatcgacg tttcgggatc atgcgcgatg cgtggtagct cgctcgccac
     1741 ccggctgttc ctgtctgcga ccgcgtgggt ggtggtgatc ctgctcatca ccggggtggt
     1801 gctgtcgtcg gtgtaccgcg acgcgaccga acgcgccttc gaccgccgtc tcaacctcta
     1861 cctccggacc ctgatcgccg aagtcgcgac cccggacgag ccgcccgacc gtcaattcca
     1921 gtcgctcggc gagccgctat tcgaactgcc gctgtccggc tggtattggc agatcacccg
     1981 caccgactcc gagaaggccg aggtgcgcgc gtcgcgctcg ctgtgggaca agaagctgcc
     2041 gaaacttgaa gaccacggcg ccgatttgac cgcggccggc at
//
LOCUS       DASWGM010000721         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2452967,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000721 DASWGM010000000
VERSION     DASWGM010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452967"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGA15_20220"
     CDS             complement(<1..240)
                     /locus_tag="VGA15_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX9590060.1"
                     /translation="MIDIAANETSAEADAGHGISFGEAFRVWLRVAVLSFGGPAGQIA
                     VMHRILVEEKNWISESRFLHALNYCMLLPGPEAQQL"
     gene            complement(237..1055)
                     /locus_tag="VGA15_20225"
     CDS             complement(237..1055)
                     /locus_tag="VGA15_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase/chromate resistance protein"
                     /protein_id="HEX9590061.1"
                     /translation="MSSHTSISPDKLARLIGTAKTPALIDVRTDEDFAADQRLIPGAV
                     RRHHQEAADWGEEFSGRSAVVVCLRGQKLAEGTAAWLRHVNVEAEALEGGFEGWKAAK
                     LPMVPASKLPARDAQGRTVWVTRARPKIDRIACPWLIRRFVDPNAVFLFVTPAEVVAV
                     GERFNAVPFDIENVFWSHRGELCTFDVMVEEFGLATPPLLRLATMVRAADTGRLDLSP
                     EAPGLLAASLGLSRMYDDDLEQLEAGIALYDAFYRWCRDATGETHNWPTNKAKP"
     gene            1320..1769
                     /locus_tag="VGA15_20230"
     CDS             1320..1769
                     /locus_tag="VGA15_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590062.1"
                     /translation="MHTFAAAGIGFLLAVLWFDLLFDVQTRKHAGDLLPQEVLASISA
                     YYRRVTTDAYPMNRLVALVMVLTLAAICAEIVQGENPWWIGWVSLALAGSGFVLTITR
                     TVPNAVRLGRGNDSPEQQSTLARAIYRDHLLSFARMIVVLGLQLTAR"
     gene            1924..>2362
                     /locus_tag="VGA15_20235"
     CDS             1924..>2362
                     /locus_tag="VGA15_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9590063.1"
                     /translation="MAAALSVDPGHAQSEKPGVEKLYILNCGEGVAGDISRWSPGVNE
                     GKSMDFVDTCYLIKHAQGWFLWDTGLSDAVAAMPNGLAPSDPKAVYWHRPKTLAAQLD
                     QLGVKPADIKAMAVSHTHPDHIGNVEMFPTTMLYVQKAEYEWPG"
BASE COUNT          435 a          735 c          733 g          459 t
ORIGIN      
        1 aagctgctgc gcctccggcc ccggcagcag catgcagtag ttcagcgcgt gcaaaaatct
       61 gctctcggaa atccagttct tttcctcgac caggatgcgg tgcatcaccg cgatctgtcc
      121 ggccgggccg ccgaaactca aaacggcgac ccggagccag acccgaaagg cttcaccgaa
      181 gctgatgccg tgaccggcat ctgcttcggc tgaagtctcg ttcgcggcga tatcgatcac
      241 ggctttgcct tgttggtggg ccagttatgg gtttcgccgg tcgcatcgcg gcaccagcga
      301 tagaacgcat cgtacagcgc gatcccggcc tcgagttgtt cgagatcgtc gtcatacatc
      361 cgcgacaggc cgagcgacgc cgcgagcagc cccggcgctt ccggcgacag atcgagccgc
      421 ccggtgtcgg cggcgcggac catcgtcgcc agccgcagca agggcggcgt cgccaggccg
      481 aactcctcga ccatcacgtc gaaggtacag agttcgccgc gatgactcca gaacacgttt
      541 tcgatatcga agggaacggc attgaagcgt tcgccgaccg cgacgacctc ggcgggtgtc
      601 acaaacagaa acaccgcatt cggatcgacg aaccgccgga tcaaccaggg gcaggcgatg
      661 cggtcgattt tcggccttgc gcgcgtaacc cagaccgtgc gtccctgcgc gtcgcgcgcc
      721 ggcagttttg acgccggcac catcggcaat ttggcggcct tccagccctc aaaaccgccc
      781 tccagcgctt cggcttcaac gttgacatgc cggagccagg ccgcagtacc ctccgcgagt
      841 ttctggccac gcaggcagac gacaaccgcc gaacgccccg aaaattcctc gccccagtcg
      901 gcggcctcct ggtggtgacg cctgaccgcg ccgggaatca gccgctggtc ggcggcaaaa
      961 tcctcgtcgg tgcgcacgtc gatcagggca ggcgtcttgg ccgtaccgat caggcgtgca
     1021 agcttatccg gagatatcga tgtgtgggat gacatggttg cgccctcgtg agaagaaacg
     1081 ggacgcgata cttgggcatg tcgcctcgtg gggggatcgc aaaaccccca gagcgccatt
     1141 acagcgaaac tgacgctggc tgtcaatgtg ctggtcggct tttgccggcg ggtagaagtt
     1201 cccgccgcgc gtagagcctt gctcacatcc gccgtcgctg gtggcgcaac attcaagcca
     1261 ggctcctgtc atcgggccca tgatgaatta tgatggccgg cgaagaaggg ggcttcgcca
     1321 tgcatacatt cgccgcggcg ggaataggtt tcctgcttgc cgtgttgtgg ttcgatctgc
     1381 tgttcgatgt gcagacccgc aagcacgccg gcgatctctt gccgcaggag gtgctggcct
     1441 cgatttcggc ctattaccgg cgcgtcacga ccgatgccta tccgatgaat cggctggtcg
     1501 ccttggtgat ggtgctgacg ctggctgcga tctgcgcgga gatcgtgcaa ggcgaaaacc
     1561 cctggtggat cggttgggtt tcgcttgcgc tggccggcag cggctttgtg ctcaccatca
     1621 cccgcacggt cccgaacgcg gtgcggctcg gccgcggcaa tgattcaccg gagcagcaat
     1681 cgacgctggc gcgcgcaatt tatcgtgatc atctcttaag cttcgcgcgc atgattgtgg
     1741 ttcttggcct gcaactgacg gcgcgataag ggcgtctggt cttcgcgcca cgtcttctgg
     1801 ccatgcgtcg tctggatgca acatttgcaa tgctgtacta tccagccatc ggggaaattc
     1861 cgggtctgtc ggcgttggag aaatgacgtg aaacgagtag gtggtggtct cgcggcggta
     1921 cttttggcag cggctttgtc ggtcgatccc ggccatgcgc aatcggaaaa gcccggcgtc
     1981 gaaaaacttt acatcctcaa ttgcggggaa ggcgtcgccg gcgatatttc gcgctggtcg
     2041 cccggggtca atgaaggcaa atcgatggac ttcgtcgaca cctgctatct gatcaagcac
     2101 gcgcaaggct ggttcctgtg ggacaccggg ctatccgatg cggtcgcggc gatgccgaat
     2161 ggccttgcgc catcagatcc gaaggccgtc tactggcatc gtccgaagac gttggcggcc
     2221 cagcttgacc agcttggcgt caagcccgcc gacatcaagg cgatggccgt ctcgcacacc
     2281 catcccgacc atatcggcaa tgtcgagatg tttccgacca ccatgctcta cgtccagaaa
     2341 gccgaatacg aatggcccgg cg
//
LOCUS       DASWGM010000722        10306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1879315,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000722 DASWGM010000000
VERSION     DASWGM010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879315"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..686)
                     /locus_tag="VGA15_20240"
     CDS             complement(354..686)
                     /locus_tag="VGA15_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590064.1"
                     /translation="MLIMLTVLVLVCSMSVTPDYLRNCDRNNAVQVMQLPEQIASPSM
                     CAMRGEAYIAGTTIGQQLAEDERVKVLCIKSRLTWDSLGANDGGESRSTIALTIDARS
                     RPIRAHLV"
     gene            complement(728..880)
                     /locus_tag="VGA15_20245"
     CDS             complement(728..880)
                     /locus_tag="VGA15_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590065.1"
                     /translation="MKVEFLSFLEGALELSILLNIFMAELCGLLIYQDRKTAVPVSNG
                     TRSRMG"
     gene            complement(1063..1248)
                     /locus_tag="VGA15_20250"
     CDS             complement(1063..1248)
                     /locus_tag="VGA15_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590066.1"
                     /translation="MTAFSDSYVLKVLLAPCTPARGLDAVARRWRKMLDPYRPELHYM
                     RGPGPKWREKRAHGTAR"
     gene            complement(1862..3091)
                     /gene="hemA"
                     /locus_tag="VGA15_20255"
     CDS             complement(1862..3091)
                     /gene="hemA"
                     /locus_tag="VGA15_20255"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX9590067.1"
                     /translation="MTYEHFFADALARLHEERRYRVFADLERIAGRFPHATWHSPDGP
                     RPVVIWCSNDYLGMGQHPKVIGAMIETATRMGAGAGGTRNISGTNHPLVELERELADL
                     HGKEAALVFTSGYISNETGIATIAKLMPNCLILSDAWNHNSMIEGVRRAGTERKIWRH
                     NDVGHLEELLAAQPKERPKLIVFEGLYSMDGDTSPVGCICDLAERYGAMTYIDEVHSA
                     GMYGPAGAGIAAREGVMERVDVVEGTLAKAFGCLGGYIAASANIIDAVRSYAPGFIFT
                     TALPPAVCAAASAAIRHLKTSQWERDRHQDRAARVKAVLGVSGLPVMPNDTHIVPVMV
                     GDPEKCKDASDLLLAEHAIYIQPINYPTVPRGTERLRITPTPYHEDALIDALAEALVD
                     VWGRLGLRLQYRALAAE"
     gene            complement(3118..4056)
                     /locus_tag="VGA15_20260"
     CDS             complement(3118..4056)
                     /locus_tag="VGA15_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX9590068.1"
                     /translation="MYMAVRVFLTLVVVAAAVFLGYEMASYYLYSPWTRDARIRADVV
                     TVAPDVSGYVTDVNVRNNQFVRKGDILFKIEQDRYRLALANAEASLAVQYAQREMLHQ
                     QFERRAKLTLGLSITAENLDNARRQFESAVANHKQATAARDVATLNLERTEVRAPVNG
                     FVTNLNNLAPGVYASQGKGVMALIDSDSYRVDAYFEETKIPRIVPGSLVQIRLMNGAG
                     TLQGQVDGIARGITDPDNKDGPELLASVNPNFTWVRLAQRIPVRIRLTEVPPNVLISA
                     GMTCTVIVKDGAAPQIGTKLKRLVSAVLDTPKVSDR"
     gene            complement(4070..4273)
                     /locus_tag="VGA15_20265"
     CDS             complement(4070..4273)
                     /locus_tag="VGA15_20265"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEX9590069.1"
                     /translation="MLADVDILGAFVPAIAIWLFVSLVIFIVPDVLLTRAGFYRLFWH
                     PALVRTSLFVCWFCAGGLAFVTM"
     gene            complement(4263..6263)
                     /locus_tag="VGA15_20270"
     CDS             complement(4263..6263)
                     /locus_tag="VGA15_20270"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX9590070.1"
                     /translation="MMTASVSTTIAGMPPRFAPAAPVRWVDNPAVVAVVFAIKAFLAG
                     LLALFIAFWLGLDEPRWALLTVFIVAQPDSGQVLAKGFYRILGTIAGVLVSIALVFGL
                     SQSGELFLAALALWIGLCNFAAAALRNFASYGFLLAGYTVAIIGISAALNPDQAFPVL
                     LARFTEIVLGIVCASLVTRLVFPRDLMPKLVGLVEALRRGAERLAAAVRRVDWQDRAG
                     ERRQFLTDFAAVETTRASAYFESPEARACNGALRQLVGAALHVSAVAEATAGRTDARS
                     GTGADPRLATLVRAAPDTPRGNGEVVSRIVDAVDARALAEAVVRLRAAAAAFDQGTAA
                     DDDAGRGFWSDPVAAALTGIRSALVVAVASAFWIVTAWPNGTIAVIVAATMCSLFGDL
                     EQPVKISLALVATLLIAFIPVFGTVYGLLPMATDFVSMSVALAPLLLACGVIIARQPL
                     GLFPVAYFTVGSNIDNVMNYDLSAFLNTSIAILMGMGFALLLFAVFFPETPRRIGNRF
                     HRQLLVRLGRLCSAEHPTVADHERALYERLAATIVRLKNEPAAARACIAGAIVALSVS
                     RAVEELRTAIATGRLPAAMTSGISRLLTAASPGFLHPSRRQIVKTAWDARALRRRALA
                     LARSVEDDQESEALVAALAGCEVLRSSLLKAPLLLREVLDAR"
     gene            6594..6824
                     /locus_tag="VGA15_20275"
     CDS             6594..6824
                     /locus_tag="VGA15_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590071.1"
                     /translation="MQAFIRLGARLELTTWHVESMSLFGDLQAIQPQHGQDSMMLRRS
                     ALKAWTVYWFKLANLCFPWVAVMSLQNDAERK"
     gene            6821..7189
                     /locus_tag="VGA15_20280"
     CDS             6821..7189
                     /locus_tag="VGA15_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590072.1"
                     /translation="MNYTVMKRAEQNSWRRSAILRVAISAGWLLCSLPAGAAPSSCQR
                     ELGTTDITLIKSVLHLRDVEHAPEDRQCAAYRQHVATVSKVRGVFARCLSGAKRDADL
                     QQLEGALDDANGVIARVCER"
     gene            7233..7673
                     /locus_tag="VGA15_20285"
     CDS             7233..7673
                     /locus_tag="VGA15_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590073.1"
                     /translation="MTVPLEPALAALAGSTIGGLTTLAVTLMTQRVQARAALTTRDLT
                     VRQKLYRKFIEEASKLYGDALMHSAVDIPMLIGTSALVNRMRVISTSGIVDKAEVVLR
                     TIVDIYFSPNKTIAELHEEINRDKLDLLRDFSNAAREELSALKH"
     gene            8920..10068
                     /locus_tag="VGA15_20290"
     CDS             8920..10068
                     /locus_tag="VGA15_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX9590074.1"
                     /translation="MKRDLRLDFFRGLSLWMIFLDHIPSHRVIDVASWVTIRNYGFSD
                     AAEIFIFVSGYTASLVYGRKMWQQGFAVGSARILRRVWQIYVAHLLLFAFYVAEVGYA
                     AAKFGAPNFENYTNTSIFFRDPGAAMAQALLLHFKPVDLDVLPVYVLVLGAFPPTLWL
                     LQRAPKLVLAGSAALYACVWLFGWNLSAYPDGLWYFNPFAWQFLFVLGAWCAAGGAER
                     LRGVTESRIAIIAAAICVVVAFFIAMGWHSRSFAAIQPSWLTQWIINHPIDKTDVHPF
                     RLLHFLALTLIVLRFVPREWVAWQAPVFRQAILCGQHSLEIFCAGVFLSFAAHVILFE
                     WSLGAVAQVAVSASGITLMICLASLLTWYRSLQLETRGLGTKSMATLR"
     gene            complement(10115..>10306)
                     /locus_tag="VGA15_20295"
     CDS             complement(10115..>10306)
                     /locus_tag="VGA15_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590075.1"
                     /translation="LPGGRPMQLGLFQGRLGFGRAIVLLEEGCEEFSNIQGLGQIRFP
                     KGNISAKFEEIRAVRGHLP"
BASE COUNT         1895 a         3170 c         3332 g         1909 t
ORIGIN      
        1 cgcagccgct atccagacgc gcgagcggcg cgtcgcctgt ttgccttttc gatcctttat
       61 ctgttcgtgc tcttcacggc gctgttggca gacgcggcgg tggcggcccg ctggccggcc
      121 tgaggcgccg ggacaattag gaggcgaaat aggcccgcgc cagaagggcg tagcgatgaa
      181 tgtcggacca ggcgcagcgc gtggttacgg gatattgcgc cgccagccgc tcgagccggt
      241 cgaggcaggc tgctcctccc tgaattcgcc cttcatcgaa atagaggcgc gtccgttcga
      301 cgattgcagc cgcggctagg cggcctctct ccgcgcgccg acggtggggg atcctatacc
      361 aaatgggccc ggatggggcg ggagcgggcg tcgatcgtca gtgcaattgt gctgcgagac
      421 tcgccgccgt catttgcgcc aaggctatcc catgtgagcc ggctcttgat gcaaagaact
      481 ttgacgcgct cgtcctcggc gagttgctgg ccgatcgtcg tcccggcaat gtaggcctct
      541 ccgcgcatcg cgcacatcga cggggaggct atctgttcgg gcagctgcat gacctgcacg
      601 gcattgttgc ggtcacaatt gcgcaaatag tccggggtga cggacatcga gcagacgaga
      661 accaacaccg tgagcatgat cagcactctt gcgattctac acgccaccca agctgtgcgg
      721 ctagcctcta tcccatgcga gaccgcgtcc cgtttgaaac gggaaccgcc gttttccggt
      781 cctggtagat caagagaccg cacagctctg ccatgaagat attgagcagg atcgatagtt
      841 ccagcgcgcc ctcaaggaag gacaaaaact cgaccttcat cgtaacctcc ttgttgtccg
      901 tcgggattcc atgcgcggca gcgcccgttc tcaatccgcc tagattaatc cgcctcaggc
      961 ggcctgttcg atcctacgga ggtgccctgt atcgtatgcc gtagatgctg tgcgggcccc
     1021 ttgtgtgaca aggcacaacg cgcctagctg atgcgggcgc agctaacgtg cggtgccgtg
     1081 tgcgcgcttt tcccgccatt tcggtccggg gccacgcatg tagtgcagct cgggccggta
     1141 cggatcgagc atcttgcgcc agcgccgggc gaccgcgtcg aggccgcgcg ccggcgtgca
     1201 gggggctagc agcaccttca agacatacga atctgagaac gccgtcattc gaacctccgt
     1261 tcgcggaagt ggcaccgatc aaaagcggga ccgggaacgg cgctgccttt tccaccgtgg
     1321 cgccctaccg gcggctcagt cgccgctttg ttgatcggct gccaaggatg ctagttcagc
     1381 gaatgccgac aagtcgatcc tacggaggtt gatccgcata acgtcttggg tcgagccgct
     1441 tatacttagg ttgggcttgg ttgtgccttg ggttgaggcg gctcctgtcg cgcgacacga
     1501 acagcttcat cggaatgcat aggggaactc agaatccgag cggcggcgga aaatcgcctc
     1561 ggaacaaaag ctatcaacag gcaagcagat cgccgtgggc ccctactttg tccctctcac
     1621 cttccccttt gtgaggacag ccaagaaggc atcaccggcg tcgtcggtga gcgtccggcc
     1681 attgctcggg aaggacgccg ggcgcgtggg gtcgtagaac agcatgtccg gcagcagcgt
     1741 ccctgccacc cgctttgctt cctccggcgt ataccacccg cgtgctccag cgcgagcgcg
     1801 aatacggcaa cgaaacgggc atcgtccgcc tgcgtcctca tggttgggtt agccagggca
     1861 gtcactctgc tgcgagcgcg cgatattgga gccgcaaccc gaggcgcccc cacacatcga
     1921 ccagcgcttc ggcgagcgcg tcgatcagcg cgtcctcgtg atagggcgtg ggcgtgatgc
     1981 gcaggcgctc ggtgccgcgc gggacggtcg gatagttgat cggctggatg tagatcgcgt
     2041 gctcggccag cagaagatcg cttgcgtcct tgcacttctc cggatcgccc accatcacgg
     2101 gcactatgtg tgtgtcgttg ggcatcaccg gtaggccgga cactccgagc accgccttta
     2161 ctcgtgccgc gcgatcctgg tgccggtcgc gctcccattg cgaggtcttc aggtgccgga
     2221 tcgccgcgct ggcggccgcg cagaccgccg gcggcagcgc ggtggtgaag atgaagcccg
     2281 gcgcatagga gcgcaccgcg tcgatgatat tggcgctcgc ggcgatgtag ccgccgaggc
     2341 agccgaacgc cttggcgagc gtgccctcga ccacgtcgac gcgctccatg acgccctctc
     2401 gcgcggcaat cccggcgccg gccgggccgt acatgccggc ggaatgcacc tcgtcgatat
     2461 aggtcatggc gccgtagcgc tcggcgaggt cgcagatgca gccgaccggc gacgtgtcgc
     2521 cgtccatcga atagagcccc tcgaacacga tcagcttggg ccgctctttg ggctgcgccg
     2581 ccagcagctc ctcgaggtgg cctacgtcgt tgtggcgcca gattttcctt tccgtgccgg
     2641 cgcggcgcac gccctcgatc atcgagttgt ggttccacgc atccgacagg atcaggcagt
     2701 tcggcatcag cttggcgatg gtggcgatgc cggtctcgtt ggagatgtag cccgaggtga
     2761 agaccagcgc ggcttccttg ccgtgcaagt cggcgagctc gcgctccaac tcgaccaggg
     2821 ggtgattggt gccggaaatg ttgcgcgtgc cgccggcgcc cgcgcccatg cgcgtcgcgg
     2881 tctcgatcat ggcgccgatc accttggggt gctggcccat gccgaggtag tcgttcgagc
     2941 accagatcac gaccggacgc gggccgtccg gggaatgcca cgtcgcgtgc ggaaaacgcc
     3001 cggcgatgcg ctcgagatcg gcgaacacgc ggtagcgccg ctcctcgtgc aggcgggcga
     3061 gcgcatcagc aaagaaatgc tcgtaggtca tgcggtggaa actccgtgcg gattggctca
     3121 acggtcggaa actttgggcg tgtcaagaac tgccgacaca agccgcttga gtttggtgcc
     3181 gatctgagga gcggcgccgt ctttcacgat caccgtgcag gtcatccccg cgctgatgag
     3241 gacgttgggc ggaacctcgg tcaaacgaat gcggacgggt atgcgctgcg ccagcctcac
     3301 ccatgtgaag tttgggttga cgctggcgag cagctcgggg ccgtccttgt tgtccgggtc
     3361 ggtgatgccg cgcgcgatgc catcgacctg tccctgcagg gtgccggcgc cgttcatcaa
     3421 gcggatctgc acgagagagc cgggaacaat gcgtggaatc tttgtttcct cgaaataagc
     3481 gtcgacgcga taggagtcgc tgtcaatcag ggccatgacg ccctttccct gggacgcata
     3541 gacgcccggt gcgaggttgt tgaggttcgt cacgaagccg ttgacgggcg cccgcacttc
     3601 ggtgcgctcg agattgagcg tcgcaacgtc gcgtgcggcg gttgcctgct tatggttggc
     3661 gacggccgat tcgaactgcc gccgcgcgtt gtcgagattc tcggcggtaa tgctgagccc
     3721 cagggtcagt ttggcccgac gctcgaactg ctgatgcagc atttcccgct gcgcgtattg
     3781 cacggcgaga gaggcctcgg cattggcgag cgccaggcga taccggtcct gctcgatctt
     3841 gaacaggatg tcgccttttc tcacgaattg attattgcgc acgttcacat cggtgacgta
     3901 gccggatacg tcgggcgcaa cggtgacgac gtcggcgcgg atgcgggcat cccgggtcca
     3961 cggcgagtag agataatagg acgccatctc gtagccgaga aatacggcgg cggccacgac
     4021 gacgagtgtg aggaacacgc gaaccgccat atacatggtc tttcccttct cacatggtca
     4081 cgaaggcgag gccgcccgcg cagaaccagc agacgaacag cgacgttcga accagcgcgg
     4141 gatgccaaaa caaccgatag aagcccgccc tcgtgagcag aacgtcagga acgatgaaga
     4201 tgacgagcga gacgaacagc cagatcgcga tggccggtac gaaggcgccg aggatgtcta
     4261 catcagcgag catccaagac ctcccgcaac agcagcggtg ctttcagcag gctcgagcgg
     4321 aggacctcgc agccggcgag cgcggcgacg agcgcctcgc tttcctgatc gtcctccacg
     4381 gagcgggcca aggcgagcgc ccggcggcgc agcgcgcgcg cgtcccaggc ggtcttgacg
     4441 atctgccggc gggacggatg cagaaaaccc ggcgaggcag ctgtgagcag ccgtgaaatt
     4501 cccgaggtca tcgcggcggg aagccggccg gtcgcgatgg ccgtcctcag ctcctcgacc
     4561 gcgcgggaga ccgacagcgc gacgatggcg ccggcaatgc aggcgcgcgc tgccgccggt
     4621 tcattcttga ggcggacgat cgttgcggcg agccgttcgt agagagcgcg ctcatgatcc
     4681 gccaccgtgg ggtgctccgc actgcacagc cggccgagcc ggaccagcaa ctgacggtga
     4741 aaccggttac ctatccgccg cggcgtctcc ggaaagaata cggcaaacag cagcagcgcg
     4801 aagcccatcc ccatgagaat ggcaatcgag gtgttgagga acgccgacag gtcgtaattc
     4861 atcacgttgt cgatattgga cccgactgtg aaatacgcga cgggaaagag gccaagaggc
     4921 tggcgcgcaa tgatgacgcc gcacgccagc agcagaggcg cgagtgccac cgacatggag
     4981 acgaagtcgg tcgccatcgg cagcagcccg tacaccgtcc cgaacaccgg gatgaatgca
     5041 atcagcaggg ttgcgacgag agcgagactg attttgaccg gttgctccag gtcaccgaac
     5101 aagctgcaca tggtggcggc gacgatcacc gcgatcgtac cgttcggcca tgcggtgacg
     5161 atccagaatg ccgaagcaac cgcgacgacg agggcggagc gaattccggt cagggccgcc
     5221 gccaccggat ccgaccagaa accgcggccg gcatcgtcgt cggcggccgt gccttggtcg
     5281 aaggccgcgg cagccgcgcg aaggcgaacc accgcttcgg cgagcgcgcg cgcatccacc
     5341 gcatcaacga tgcgggacac gacttcgcca tttccgcgcg gcgtgtccgg cgccgcacgg
     5401 acgagcgtgg cgaggcgcgg atcggcgcca gtgccggatc gcgcgtccgt tcggccggcc
     5461 gtggcctcgg cgacggcgct cacgtgcagg gccgcgccca ccagctgccg aagcgcgccg
     5521 ttgcaggccc gggcctcggg gctttcaaaa taggcggacg cgcgcgtcgt ctcgaccgcc
     5581 gcgaagtcgg tgaggaattg ccggcgttcg ccggcgcggt cctgccaatc gacgcgcctg
     5641 acggctgcgg caaggcgttc ggcgccgcgg cgcagcgcct caaccaggcc gacgagcttc
     5701 ggcatgaggt cgcgcggaaa caccaggcgg gtcaccagcg acgcgcagac aattccgaga
     5761 acgatctcgg tgaatcgcgc caacaaaacc gggaaggctt gatccggatt gagggcggcc
     5821 gatatgccga tgatggcaac cgtgtagccg gccagcagaa atccataaga agcgaagttg
     5881 cgcagcgcgg ccgccgcgaa attgcacagc ccgatccaca gcgccaaggc agcgaggaag
     5941 agttcgcccg actgggacag cccgaacacc agcgcgatcg acaccagcac cccggctatc
     6001 gtgccgagga tgcggtagaa gcctttggcg agcacctggc cgctgtcggg ctgagcaacg
     6061 atgaacacgg tgagcagcgc ccacctgggt tcgtcgagcc caagccagaa agcgatgaag
     6121 agggcaagga ggccggccaa aaaggctttg atcgcgaata caacggcgac gactgccgga
     6181 ttgtccaccc aacgaaccgg tgccgccggc gcgaagcgcg gcggcatgcc tgcaatggtc
     6241 gtcgaaacgc tcgctgtcat cataacgccg tcgtccatcg tcccggcgga gggggcggcc
     6301 ggtcctcggg ggaaagggac cggccgccgg ctagcccggc gtgggacgac gagggggagc
     6361 gcgccggggg cgccaagcac ttgcgaatgc cgcccgtctt cgtggccaaa tcggtacgcg
     6421 acaaggcgcg ccggtgcgag tgaagcattc caaatctcgg caaatcgtgg cgaatgccga
     6481 tgccatttgc atcgaccgcc gtcgtaacac actgtccgcg gcccgtttga aggaaccggc
     6541 tgaggcttta agccgatgag ctgccctcag gatttgcgac gatttagggt catttgcagg
     6601 cattcattcg cctcggcgcg cggctcgagc taacaacttg gcatgtagag agcatgtcgt
     6661 tgtttggtga cctgcaggcc atccagccgc agcacggaca ggattccatg atgctgcgcc
     6721 ggtcggcact gaaagcgtgg acggtctact ggttcaagct ggcgaatctc tgtttccctt
     6781 gggtcgccgt catgtctctg cagaacgacg ctgagcgaaa atgaactaca ccgtcatgaa
     6841 acgagcggaa cagaacagct ggcgacgatc cgcgatactg cgggttgcaa tctccgctgg
     6901 atggctgctg tgcagccttc cggcgggagc cgccccatcg tcgtgccagc gcgagctcgg
     6961 caccaccgac atcaccttga tcaagtctgt tctgcacctg cgcgacgttg agcatgcgcc
     7021 ggaagaccgg caatgcgcgg cctatcgcca gcacgtcgct accgtgagca aagtccgagg
     7081 ggtatttgcg cgatgtcttt cgggcgcgaa aagggacgcc gatctgcagc aactcgaggg
     7141 cgccctcgat gacgccaacg gcgtgatcgc gcgggtttgc gagcgatgaa gcgaagagtt
     7201 cgacctgagt ctgtggatct ggagggcgcg ccatgacggt tccgcttgaa cctgcgcttg
     7261 ccgcgctcgc cggttccacg atcgggggcc taaccacgct ggcagtgacg ttgatgaccc
     7321 agcgggtcca ggcccgggcg gcgctcacga cacgggacct gaccgtccgg caaaagctct
     7381 acagaaaatt catcgaggag gcctcgaagc tctacggcga cgcgctgatg cacagtgcgg
     7441 tcgatatccc gatgctcatc ggcacctccg cgctcgtcaa ccggatgcgc gtcatttcga
     7501 cctctggaat cgtcgacaaa gccgaggtcg tcctgcggac catcgtcgac atttactttt
     7561 cgcccaacaa gaccatcgcc gagttacatg aggagatcaa cagggacaag ctggatctcc
     7621 tgcgcgactt cagcaacgcc gcgcgagagg agctgtcggc gctgaagcat tgagactcgt
     7681 ccatccatgc tgtcggcgcg tcacgtaatt tgctgccact cgcgggttct ttgcgaccat
     7741 ttgcatcgtc tttcacctct taactcgaac ggatgcgcgc ccaccttggt tggtcgagcc
     7801 cgagtcagaa cgcgaggaat aaggcaagga ggccggccaa gaaggttttc agcgcgaata
     7861 cgacggcgac gacggcgaga ttgtccgccc agcgaaccgg tgccgcccgc gcggaccgcg
     7921 gcgacgtgcc tgggaggagc gtcgaagcac ttgctgccat catagctcgg ttcgttcgct
     7981 tgcgacgttc gccggattag agagtgttca tacacatgcg atcagcgtcg tcctccgtcg
     8041 tcccagagga ggggcggccg gtcctcgggg ggacggaccg gccgccggcg agcccggcgg
     8101 ggggaggacg ccgggatgcc aagcacgtgc gcaagccgcc tgtctgcgtg aggcaattgg
     8161 tgtacgagac ggcgcgccgg cgcgagtgaa gcagtgcaaa taccagcaaa tcgtggcgaa
     8221 tgacccgcgg agacagggta aactggatct cttgcgtgac ttcagcagtg ccgcgcgaga
     8281 ggagatgtcg gcactgaagt attgaggttc accgatccgt gtcgccgatc gccgggaatt
     8341 tgctgccgcc ctgacttctt tgtgatcatt tgcaccctct tgcgcctctt aagtcgaaga
     8401 gatgcgtgaa gtcgcctaac ttgagatcag ataacgcggc tttttcgcac gcaggatgcc
     8461 gcggctgact ctggaggcgc ccatggtccg cgagcagatg atcaatttct tgcaggcgac
     8521 tgtgatcttc ctgctgatca ccggtatcca agaggagtaa ggcatgacgc cgattggtga
     8581 caattggtgg ttgttggtga tgggtattat cggcatgctg tgctggatcc tgggcgtaat
     8641 cccatggcaa ttgtgagcga ctgccatggc ggaatcggcg tcattggcgc cgtcaggtct
     8701 tctaggtcca ttggcgttcc cgtccctgca acacgaccct aagaaatcca gggcaagcgc
     8761 tgtcacgcag aatcaacgcg atctcgacaa gcagctcaat atttgccgcg gctgttggtc
     8821 ggttccgacc tcacccacgc gtcacgcggg cgcgggcgtc gaaaagcgcg gtcgcacgcc
     8881 aatataaaac gtggatggct cttgagattc ggtgccgcga tgaagcgtga tcttcggctg
     8941 gatttctttc gaggattgtc gctttggatg atcttcctcg accacattcc ctcgcatcgc
     9001 gtgatcgatg tcgccagctg ggtcaccatc cgcaattacg gtttcagcga tgcggcggag
     9061 atcttcatct ttgtttccgg gtacacggct tccttggtct acggccgcaa gatgtggcag
     9121 cagggtttcg cggtcggcag cgcccggatt ctgcgccgcg tttggcagat ctatgtcgcc
     9181 catctgctcc tgttcgcctt ttacgttgcg gaggtgggat atgccgccgc gaaatttggc
     9241 gctcccaatt tcgagaacta caccaatacg tcgatcttct ttcgcgatcc tggggccgcg
     9301 atggcccaag cattgctgct gcacttcaag ccggtggatc tcgatgtgct ccccgtctac
     9361 gtcctcgtgc tcggcgcttt cccgccgacc ctctggctcc tgcagcgcgc gccgaagttg
     9421 gttcttgccg gatcggcggc cctctatgca tgcgtatggc tgttcggctg gaatctttcg
     9481 gcttatccgg acgggctctg gtatttcaat ccattcgcct ggcaattcct gttcgtgctc
     9541 ggggcttggt gcgcggcggg tggcgcagag cgcctgcgtg gcgtgaccga atcccgcatc
     9601 gccatcatcg ccgccgcgat ctgcgtcgtg gtggcgttct tcattgcaat gggctggcac
     9661 tcgcggtcct tcgcggcgat ccagccgtcc tggctgacgc agtggatcat caaccatccg
     9721 atcgacaaga ccgacgtgca tccatttcgg ctcctgcatt tcctggcgct cacgctcatc
     9781 gtcctgcgtt tcgtcccgcg tgaatgggtc gcttggcaag cgcccgtgtt ccgccaagct
     9841 atcctatgcg ggcagcactc actcgagatc ttctgcgccg gagtattcct gtcgttcgca
     9901 gcgcacgtca tcctgttcga atggtcgctc ggcgccgtcg ctcaagtggc cgtgagcgct
     9961 tcaggcatca cgctgatgat ttgcttggct tccttgttga cttggtacag gagtctgcag
    10021 ttagagactc ggggattggg cacgaaatcg atggctacgc ttcgataacg aatgaaggga
    10081 catcggatcg ttggcaggga acgccgcgca ggcttcaagg gagatgcccg cgaacggctc
    10141 tgatctcttc aaattttgcg ctgatattcc cttttgggaa acggatttgc cccagcccct
    10201 ggatgttgga gaattcttcg catccctcct cgagaagcac tattgcgcgc ccgaagccga
    10261 gccgcccctg aaacagtccg agttgcatag gtctccctcc ggggag
//
LOCUS       DASWGM010000723         4769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2453245,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000723 DASWGM010000000
VERSION     DASWGM010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453245"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(463..639)
                     /locus_tag="VGA15_20300"
     CDS             complement(463..639)
                     /locus_tag="VGA15_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590076.1"
                     /translation="MTPHIPEEFSTRLIDRSPFVIMGNTMPPRDPDEDEEDEDEEEED
                     RADEPPIVREPDED"
     gene            781..1011
                     /locus_tag="VGA15_20305"
                     /pseudo
     CDS             781..1011
                     /locus_tag="VGA15_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1014..1142
                     /locus_tag="VGA15_20310"
                     /pseudo
     CDS             1014..1142
                     /locus_tag="VGA15_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
     gene            1582..3444
                     /locus_tag="VGA15_20315"
     CDS             1582..3444
                     /locus_tag="VGA15_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9590077.1"
                     /translation="MSKPNGDDRHRHDLLQTILKGHFASDDAELIASIKAAAEFIDLP
                     SGDVLFHQGEYSDDVYFVLSGRLLALAEANGESKILGEIGRGETVGELALFTGEPRSA
                     SIIALRDSSVVKVTRQVVERALAKSPQVALQMTRIVIDRFRRSERERQAPTVPVNICI
                     LAVSPGVDAAALARRLRAAGRDSAATRIVGPDDIGQQFGTDAASAVRQNHDAIAHHID
                     GIEAASKAVYLVADPCESAWTQFCLQNCDEILLVGDGRQDPGLSSVESRYLAGETPVS
                     IARRTLVLLHGLETKSPTGTAQWLKNRRTLRHFHIRPELARDMRRLARIISGNGVGLV
                     LAGGGARGFAHVGVMKALEEAGIEADFVGGTSIGAIMGTCLALDLPAERISTAVHKAF
                     LLHPKGNITGDYNLIPLVSLIRGKRTHEAMVQAIENATGASIDAEDTWKTFFVIASDF
                     STNSEAVLDKGNLARNVIASYAVPGALPPMFIGGHLMFDGGMFNNFPVDVMARLGARQ
                     IIGVDLLIEDGRVFEIDQIPGTIAHLRDKLRSWDMQRYRLPTVPETMLNSSFISSVSK
                     QKAMRKFVDLLFQPRIEQVGLLQWKRYDDVVAAGYNHAKQVLAAESEDKLRAFR"
     gene            4092..>4769
                     /locus_tag="VGA15_20320"
     CDS             4092..>4769
                     /locus_tag="VGA15_20320"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX9590078.1"
                     /translation="MRALIRQMSTENQLWGAPRIHGELLKLGFSVAQSTVAKYMVKRR
                     GPPSQGWRTFLRNHAPDIAAMDLFVVPTIGFNLLYGFVIVRIDRRDLVWINVTTNPAA
                     DWIARQITEAFPWDGAPGYMIRDRDRIYGAVVTRRLRAMGIRDKPIAPASPWQNGFAE
                     RLIGSIRRECLDHILVSGEAHLRRNLISYADYYNSVRTHRSLHKDAPIFRPIQQTGII
                     RSHPILGG"
BASE COUNT          936 a         1446 c         1436 g          951 t
ORIGIN      
        1 accggcgccg accacgctcg cgcagatgcg ccgtcccgac agctaagcat ccctgcaacc
       61 gacacactca acaacagtct caatcatcaa cccgagctat ggctgcctgc cagccgcgta
      121 accaaccgcc tcgaaggaca ccgcgctccc gcgctcacac cttccgcagc agccaaatcc
      181 ccatagcgga gtgcgcggca ttctccgcgc cccatccccc gcgggttctt tccttggcgg
      241 ctttcggacg ccggccactg gtgtatgtcg cgccgctgtc gctggccggc atccgaaacc
      301 cttcacagga gcggaaacat tcactgtctt tgcgcaagtc cgccgcgcgc tcggccgaaa
      361 ccatctgcgt cacaatcggc gaaatcctcg agcatttacg cccaccgaat gcgccaacta
      421 tttccgaaac tcaggctatg cccaatccta aagcatcatg ctctagtctt cgtccggctc
      481 gcgcacgatc ggcggttcgt cggcgcggtc ctcttcctct tcgtcctcgt cctcttcgtc
      541 ttcatcagga tctcggggcg gcatcgtgtt gcccatgatc acgaagggac tcctgtcgat
      601 cagcctggtg gaaaattcct cggggatgtg tggggtcatg ttgttgctcc tcgggtgtgg
      661 gccctagggg attttacccc ttctgctccg gtttccgcat gagggttagc gccgcaccgt
      721 ttgcggggca tgccagcggt ggtccgcctg cggcggctca tgttcgtgat gccgagctaa
      781 gaggcgggca tctcggtcac gccgccaaag ccgatgacct ccaactcaaa gtcggaaggg
      841 cgcttcggca agcaggactt cgtctatgtc ggcgcggacg atatctatct ttgtgccgcc
      901 ggtgagcggc tgtcttatcg ctataccaat gaagaggacg gcaagacact acgccgctat
      961 tggacgaccg cctgtcaggc gtgcacgagc aaatgcacaa caggcaaaaa gagcgcatgg
     1021 ccgggctcgg caaatggccg ggctaagaat tccaggcggc gctcaactgg cacacggtca
     1081 agctgatcaa ggaaaagtat aatattccgc tggtgcttaa aggcatcgcc acggccgagg
     1141 acacattgcg gtagatcggt gtagtaagcg atcgcctcgt cgtagtcccc cacgaacaag
     1201 gtccgcaggc cgatccgttg gctcataaag ggctccaatt gcgaccagag cgtgcgaggg
     1261 tttcctcgcg gcgtgctgca ggttttgggc cggcgccgct atagccagcg ccgcacgcgc
     1321 gagcggtagc ggcgcatcgt cgccgtccct gcgcggatgc gcggaatggg gaggtgaagc
     1381 gcgctgcccg gcgaccatcg ggagggcatg acgcgttgca gcagaagtga gtttgcgccg
     1441 ccgcaatcac gacggtcaat cgatgcggcc cggtccgcca ggccgcatgg atgcttcagc
     1501 tgccggtctc gattgaaagg ggattgcgaa ctgtaaaacg atttggcaga cctttgtggt
     1561 ccaccgatgg aatgcacgtc catgtcgaaa ccgaacgggg acgatcgaca cagacacgac
     1621 ctgctgcaga ccattttgaa aggccacttc gcctcggacg acgccgagct gatcgcaagc
     1681 atcaaggccg cagccgaatt catcgacctg ccgtcgggcg acgtcctgtt ccatcagggc
     1741 gagtacagtg acgacgtcta tttcgtgctg tcgggccgat tgctggcgct cgcggaagcc
     1801 aacggcgaaa gcaaaatact cggcgaaatc ggccgcggcg aaaccgtcgg tgaacttgcc
     1861 ctgttcaccg gcgaaccacg ctccgcttcc atcatcgcat tgcgcgattc atcggtcgtc
     1921 aaagtgacgc ggcaggtcgt cgaacgggcg ctcgccaaat ccccgcaggt cgcgctgcag
     1981 atgacgagaa tcgtcatcga tcggtttcga cggtcggaac gagagcgtca ggccccgacc
     2041 gttcctgtca atatctgcat tcttgccgtc tcgcctggcg tcgacgcagc ggctttggcg
     2101 agacgcttgc gagccgcggg gcgggacagc gcagccacga ggatcgtggg tcccgatgac
     2161 atcggccagc agttcggtac ggatgccgca agcgcagtac gccaaaatca tgacgcgatc
     2221 gctcatcaca tcgacgggat cgaggccgcg agcaaggcgg tttatctcgt cgccgaccca
     2281 tgcgagtccg cctggacgca gttctgtctg cagaactgcg acgaaatctt actggtcggg
     2341 gatggccgcc aggacccggg cctttcaagc gtggaaagcc gctatctcgc gggcgagacg
     2401 ccggtctcga tcgcgcggcg gacactcgtc cttctgcatg gcctcgagac caaaagcccg
     2461 accggcaccg cgcaatggct gaaaaacaga cgcaccttga ggcatttcca catccgaccg
     2521 gaacttgcgc gcgacatgcg ccggttggcg cggatcatat cgggcaacgg tgtcggcctc
     2581 gtgctggcgg gcggtggggc gcgcggattc gcccatgtcg gcgtcatgaa ggcattggag
     2641 gaggcgggca tcgaagccga ttttgtcgga ggcacatcga tcggcgccat catggggacc
     2701 tgcctcgcgc tcgatctgcc ggcggagcgg atttccaccg ccgtccacaa ggcttttctg
     2761 cttcatccaa agggcaacat caccggcgac tataatctca ttccactggt gtcgctgatc
     2821 aggggcaagc gcacccacga ggcgatggtc caggccatcg agaacgcgac tggtgccagt
     2881 atcgacgcgg aggacacctg gaagacgttc ttcgtcattg cctccgattt ttccaccaac
     2941 agcgaggccg ttctcgacaa aggaaatctc gcgcgaaatg tgatcgcgag ctatgccgtt
     3001 cccggtgcgc tgccgccgat gtttatcggc ggacacctga tgttcgacgg tggcatgttc
     3061 aataattttc ccgtcgacgt gatggcgcgg ctcggcgccc ggcagatcat cggtgtcgac
     3121 ctcttgatcg aggatggccg ggtgttcgag atcgatcaga taccgggcac cattgcgcat
     3181 cttcgtgaca aactccggtc gtgggacatg cagcgctacc gcctgccgac ggtgcctgaa
     3241 acaatgctga actcgtcctt catcagttcg gtctcgaagc agaaggcgat gcgaaaattc
     3301 gtcgatcttc tgtttcagcc gcgcatcgaa caggtcggac tgctgcaatg gaagcgatac
     3361 gacgacgttg tcgcggcggg atacaatcac gccaagcaag tgctcgcggc ggaatccgag
     3421 gacaagctga gagcgttccg gtagtagcct gacgtgtatc catcagcaaa gtgctttatc
     3481 cggcaacact cacgccaagt ttgaagcgag cgtccgcttg gtcgagcctg cgattttgcg
     3541 acgcagggtg acgggtgcgc ataagccgcc ggcgggacta ttccggccga ggtgctcaag
     3601 cgcgccgacc atgtcatcca gcgtgattca ctcccgaggc cggcgcctca cttgtggttt
     3661 gcgcttacgg cgaatgactg ctcagggttc aaaagcaccg ttttcacggt cggccgacct
     3721 ctgtgtaccg aaaaccttat ccggattttt ggcgtaacag aatcggctaa actggcgcga
     3781 tgatcgcgct gatttggttt gttctggccg tgctggcctc gccgttcgag tcgaagagca
     3841 ggcttgaggc ggagaatgcg gtgctccgac atcagctgat cgttttgcgg cgcaaggtga
     3901 ggggtcgagc ccagcttacc aacaacgacc gctggttcct ggtccagacg tatcgatggt
     3961 ttccatcgat cctgacggtc gttacgattg tccaacctga aacgctggtg cggtggcatc
     4021 gggccggctt tcgtcgctat tggcgttgga aatcgagctc gcggggaggg cgcccgcgaa
     4081 tcgagatgga attgcgcgcg ctgatccggc aaatgagcac ggagaaccag ctttggggtg
     4141 cgccacgtat ccacggcgaa ctgctcaagc ttgggtttag cgtcgcgcaa tcaaccgtcg
     4201 ccaagtacat ggtcaagcga cgtggacctc caagtcaggg atggcggacc tttctgcgaa
     4261 accacgctcc ggacattgcc gccatggatc tgttcgtggt cccgaccatt ggcttcaatc
     4321 tgctgtatgg cttcgtgatt gtacggattg atcgcagaga tctcgtctgg atcaacgtca
     4381 caaccaaccc ggcggcggat tggatcgcac gccagatcac cgaggctttc ccttgggacg
     4441 gtgctccggg ttacatgatc cgtgatcgcg atcggatcta tggcgccgtc gtcacacgcc
     4501 gattgcgagc catgggcatt cgggacaaac cgattgcacc ggcctcacct tggcaaaatg
     4561 gctttgccga acggctgatc ggatcgatcc gacgcgagtg tttggaccac atccttgtct
     4621 cgggcgaggc acatctgcgc cggaatctaa tttcctatgc cgattactac aactccgtca
     4681 gaacgcatcg gtcgttgcac aaggatgcgc cgatttttcg tccgattcaa cagaccggaa
     4741 tcattcgttc acacccgatc ctcggcggg
//
LOCUS       DASWGM010000724         3224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2309945,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000724 DASWGM010000000
VERSION     DASWGM010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309945"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /gene="mmsB"
                     /locus_tag="VGA15_20325"
     CDS             <1..961
                     /gene="mmsB"
                     /locus_tag="VGA15_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004682330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HEX9590079.1"
                     /translation="IDLSVGSVAGFVGAIAAVLMVRYGVHFIPAAIACLALGALIGAA
                     QGYWVAYFKIPSFIVTLAGMLVFKGLALAVLQGQSVGPFSPIFQKLSSGFIPELFPGA
                     GALYPTSLLIGAALALGLVYVSVKTRARRASHGVEVEPTGFFALKNLILFGVIIYFTY
                     LIASHRGLPNVLVIMIALIALYGFVTARTTIGRQIYAVGGNEKAAKLSGIKTERLTFL
                     TFVNMGVLAALAGLVFAARLNTATPKAGAGFELDVIAACFIGGASAYGGVGRVAGAVI
                     GALIMGVMNNGMSILGIGIDYQQVIKGLVLLGAVCLDVYNQRR"
     gene            complement(1114..1434)
                     /locus_tag="VGA15_20330"
     CDS             complement(1114..1434)
                     /locus_tag="VGA15_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009032205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9590080.1"
                     /translation="MRSVLRQIRVLVVAAAGLSGSLAMAADPDHGSDLAKRWCAARHV
                     VNADQKRANPDAPPFEAIASKSDFTPEKIAFFLLDPHPKMPNFPLSRSEAADLAAYIG
                     SLRK"
     gene            complement(1419..1859)
                     /locus_tag="VGA15_20335"
     CDS             complement(1419..1859)
                     /locus_tag="VGA15_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590081.1"
                     /translation="MLGTEGKPNIIAAIRHSVAIVFGFVLIRHLAKPAQHVPVVEYSP
                     LEIAGAAERNGAGVPQEFPLPLRRLYRDRRTCAVNGFSGRKGTVDEIKSQCHETCDFG
                     HEMPALRPSPDVQPIWRMTEPIALVPMNSVRHLTSKENVGCARF"
     gene            1997..2926
                     /locus_tag="VGA15_20340"
     CDS             1997..2926
                     /locus_tag="VGA15_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9590082.1"
                     /translation="MTAIRVAIIGLGKIARDQHIPAIAETAGIELAAIASRNASIDGI
                     EHFATLDELLDGMPDIHAVALCTPPQVRHAQAAAALKAGKHVLLEKPPGATVSELTPL
                     VAVARQTGRTLFATWHSRFAPAVEPARAFLADRQIRSVVVEWKEDVKVWHPGQAWIWE
                     PGGLGVFDPGINALSILTRILPRTFFLREAELSFPSNRAAPIAANLAFSDGTGLSILA
                     EFDWRQTGPQTWDIRVDTDAGRLTLSSGGSRLVHDDRTLVDAKQAEYRGVYQRFVELI
                     ASRVSDVDLSPLVHVADAFMLGRRREVEPFIED"
     gene            2953..>3224
                     /locus_tag="VGA15_20345"
     CDS             2953..>3224
                     /locus_tag="VGA15_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HEX9590083.1"
                     /translation="MPKVVREAFGHLPDGAAGEMVKLRGDNGFEVRIITYGAAMQSIF
                     TPDRAGRVADIVLGRDDLEGYIALRRFLGATIGRYANRVANGAFEL"
BASE COUNT          543 a         1019 c          996 g          666 t
ORIGIN      
        1 catcgatctt tccgtcggct cggtcgcggg cttcgttggc gcaatcgccg cggtgctgat
       61 ggtgcggtac ggcgtgcatt tcattccagc cgccatcgcc tgcctcgcgc tcggtgcgct
      121 gatcggcgcg gcgcagggct attgggtggc ctatttcaag attccgtcct tcatcgtcac
      181 gctggccggc atgctggtgt tcaaagggct ggcgcttgca gtgctgcaag gccagtcggt
      241 cggcccgttc tcgccaattt tccagaaact ctcctccggc ttcattccag aactgtttcc
      301 cggcgccggc gcgctctacc ccacctcgct cctgatcggc gctgcgctgg cgcttggact
      361 ggtctatgtc agcgtcaaga cacgcgcccg gcgcgcaagc catggtgtcg aggtcgagcc
      421 caccggcttc tttgcgctta agaacctaat cctgttcggc gtgatcatct atttcaccta
      481 tctgatcgcc tcgcatcgcg gcctgcccaa cgtgctggtg atcatgatcg cactgatcgc
      541 gctctatggc tttgtcaccg cgcgcaccac cattggccgg cagatctatg cggtcggcgg
      601 caacgagaag gcggcaaagc tctccggcat caagaccgaa cggctgacct tccttacctt
      661 cgtcaatatg ggcgtgctgg ccgcgctcgc tggtctcgtg ttcgccgcgc ggctcaacac
      721 cgcaacaccg aaagccgggg ccggcttcga gctcgacgtc atcgccgcct gcttcattgg
      781 cggtgcctcg gcctatggcg gcgtcggccg ggtggccggc gctgtgatcg gcgccttgat
      841 catgggcgtg atgaataacg gcatgtcgat cctcggcatc ggcatcgact accagcaggt
      901 gatcaagggg ctggtgctgc tcggcgcagt atgtcttgat gtctacaacc agcgacgctg
      961 aggcgcggat tgatcaaaat cgctccaggc cggtcgggcc gggttgatcg aaatcgccag
     1021 ccgccgcggt ccgctggtac cgcagtggac aaataagccg ccaaaaatgg ccccgcccag
     1081 cgcctgctca ggcttatcct cgcgtcgtgc gttctatttg cgcagcgagc cgatataggc
     1141 ggcgagatcg gccgcctcgc ttcggctcaa cggaaaattc ggcatcttcg ggtgcgggtc
     1201 gagcaggaag aaggcgatct tttccggcgt aaagtccgac ttgctcgcga ttgcttcaaa
     1261 cgggggagcg tcggggttgg cccgcttctg gtcggcgttc accacgtggc gggcggcgca
     1321 ccagcgcttg gcgaggtcgg agccatggtc cgggtcggcg gccatcgcca gcgatccgct
     1381 cagcccggct gcggcaacaa ccagaacccg aatttgtctc aaaaccgagc gcatccgacg
     1441 ttctccttcg atgttagatg acgtacgcta ttcatcggca ccaacgcgat gggttcggtc
     1501 atccgccata tcggttgaac gtctggcgaa ggccgcaaag cgggcatttc atgaccgaaa
     1561 tcacacgttt cgtggcattg gcttttgatc tcatcgacgg tgcccttgcg gccggagaag
     1621 ccgttgactg cgcaagtcct gcggtcgcga tacaaacggc gcaagggcag tggaaattcc
     1681 tggggcacac cggcgccgtt gcgttcagcc gcaccagcga tttcgagagg ggaatattca
     1741 accaccggca cgtgctgcgc cggtttggcc aggtgccgga tgagtacaaa gccgaaaacg
     1801 atggccactg aatgccggat cgccgcgatg atatttggct tgccttcagt gcctaacaat
     1861 ccggcgatga tttctgtatt tcgcggggcc gattaggtcg gtttgcgtcg ctttgtcgcc
     1921 ggcgtttcgc tagagtgcct tttgccatac tcgtgtatcc cgccgcgttg atcctcaaaa
     1981 gccgggacaa gtcgccgtga ctgccatccg cgttgccatc atcggtctcg gaaagattgc
     2041 cagagaccag cacattcccg caattgccga aactgcgggc atcgaactcg ccgccattgc
     2101 cagccgcaat gcctccatcg acggcatcga gcatttcgcg accctcgacg aattgctcga
     2161 tggtatgcct gatatccatg ccgttgcact ctgcacgcca ccgcaggttc gccacgcgca
     2221 ggcggcggcg gcgctgaagg ccggcaaaca cgtcctgctc gaaaagccgc ccggggcgac
     2281 ggtcagcgaa ctcacgccgc tggtcgccgt ggcgcggcaa accggacgaa cgctgttcgc
     2341 cacctggcat tcccgcttcg cgccggcggt cgaaccggcg cgagccttcc ttgccgaccg
     2401 tcagatcagg tcggtcgtcg tcgaatggaa ggaagatgta aaagtctggc atccgggtca
     2461 ggcctggatc tgggagccgg gcgggctcgg ggtgttcgat cccggcatca atgcgctgtc
     2521 cattctgacc cggattctgc cgcggacgtt ctttctcaga gaggccgaac tatccttccc
     2581 cagcaatcgc gccgcgccca tcgcggccaa tcttgccttt tccgatggca ctggtctgtc
     2641 gattttggcg gagttcgatt ggcgccagac cgggccgcag acctgggaca tccgcgtcga
     2701 caccgatgcc gggcgcctga cgctgtcctc tggcggcagc cggcttgttc atgatgaccg
     2761 aacgctggtt gatgccaagc aggccgagta tcgcggcgtc taccagcggt ttgtcgaact
     2821 gatcgccagc cgggtatccg acgtcgatct ttccccgctg gttcatgtcg ctgacgcctt
     2881 catgctcggc cggcgccgcg aggtcgagcc cttcatcgag gactgagcat ggcgcggccg
     2941 gacaaaccac tgatgccgaa agtcgtacgc gaagctttcg gacatctgcc ggatggcgcg
     3001 gcgggcgaaa tggtcaagct gcgtggcgac aacggcttcg aggtgcgcat cattacctat
     3061 ggcgcggcga tgcagtccat cttcacgccg gatcgcgccg gccgcgttgc cgacatcgtc
     3121 cttggccgcg atgaccttga aggttatatc gcgcttcggc gcttcctcgg cgcgacgatc
     3181 gggcgttatg ccaaccgtgt cgcgaacggt gcgttcgaac tgga
//
LOCUS       DASWGM010000725         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1583657,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000725 DASWGM010000000
VERSION     DASWGM010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583657"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VGA15_20350"
     CDS             complement(<1..435)
                     /locus_tag="VGA15_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9590084.1"
                     /translation="MSRETPVMDLGEKPAGGTALLEIRGLKTHFATDDGIVHAVDGVD
                     LTVERGETVCVVGESGCGKTVTALSVLKLIAMPQGRIVGGQILWQGRDIVPLPADEMN
                     RIRAREIAIVFQEPMTSLNPVYTIGDQIGEVIALHEGLNRRAV"
     gene            complement(436..1377)
                     /locus_tag="VGA15_20355"
     CDS             complement(436..1377)
                     /locus_tag="VGA15_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590085.1"
                     /translation="MAGLTHASPIVDVAVPLEGRPRRISPGFEAWRKFRRHRMAVASV
                     VILAMLTSAVVLGPFVWRLPINEIDFTARLTSPSWQHPFGTDDLGQDLLARMLYGGRI
                     SLAVGFAAMAVAIVAGTLIGAISGISRGSVDAVLMWITDLFLALPQLPLLLLVIYLFR
                     DFLKNLVGPEGGVFILIVMVISAFRWMPVARLVRAQFLSLREKEFVEAARALGATTPR
                     LVIRHILPNSLGPVIVAGTIDVAAAIIAESTLSFLGLGFPPDIPTWGRLLFDAKDYLD
                     IAPHWALFAGAAIFLTVLAINFIGDGLRDALDPRKVM"
     gene            complement(1343..>1887)
                     /locus_tag="VGA15_20360"
     CDS             complement(1343..>1887)
                     /locus_tag="VGA15_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590086.1"
                     /translation="GFSLPTFFTGLLLILIFSVNLGWLPFVYRVDIPATGWHWYWENF
                     KQAIMPVAVLGLFQAASYTRYVRSAVLDVIRLDYVTTARSKGLDEGKVIVKHVVRNAL
                     IPVVTLVALQMPAVFGGAIVTEQIFRIPGIGSLLITAILANDTPVIMAVTFVFACLVV
                     VFNLLADIIYGWLDPRISYR"
BASE COUNT          434 a          575 c          586 g          292 t
ORIGIN      
        1 cacggcgcga cggttcaatc cttcatgcag ggcgatgacc tcgccgatct gatcgccgat
       61 ggtatagacg ggattgagcg aggtcatcgg ctcctggaac acgatggcga tttccctggc
      121 gcgaatgcgg ttcatctcgt cggccggcag cggaacgatg tcgcggcctt gccacaggat
      181 ctgcccgccg acgatgcgcc cctgcggcat cgcgatgagc ttcaggaccg agagcgcggt
      241 gacggtcttg ccgcagccgg actcgccgac gacgcaaacc gtctcgccgc gttcgacggt
      301 gagatcgacg ccatccaccg catgcacgat gccgtcgtcg gtggcgaaat gagtcttgag
      361 tcctctgatc tcgagcagcg cggttccgcc cgcgggtttc tcgccgagat ccatcacagg
      421 cgtttcacgc gacatttaca tgactttcct gggatccagc gcgtcgcgca atccgtcgcc
      481 gatgaaattg atggcaagca cggtcaggaa aatggcggcg ccggcgaaca gcgcccaatg
      541 cggtgcgata tccagataat ccttggcatc gaacaacaga cggccccagg tcggaatatc
      601 aggcggaaag ccgaggccca ggaacgacag cgtcgattcc gcgatgatcg ccgccgccac
      661 gtcgatggtg ccggccacga tgaccggtcc gagcgaattg ggcaggatat gcctgatcac
      721 cagacgcggc gttgtcgcgc cgagtgcccg cgccgcttcg acgaactcct tctcgcgcaa
      781 cgacaggaac tgcgcccgca ccagtcgcgc caccggcatc cagcgaaagg cactgatcac
      841 catgacgatc agaatgaaaa cgccgccttc gggaccgacc agatttttta ggaaatcccg
      901 gaacagatag atcaccagca acagcagcgg caattgcggc aacgccagaa acaggtcggt
      961 gatccacatc aacacggcgt cgacgctgcc ccgggaaatg cctgatatcg cgccgatcag
     1021 ggtgccggcg acaatggcga cggccatggc cgcgaaaccg accgcgagcg aaatacgccc
     1081 gccatacagc atgcgcgcca agaggtcctg accgagatcg tcggtgccga acggatgctg
     1141 ccatgacggc gaggtgagcc gcgcggtaaa atcgatttcg ttgatcggca agcgccatac
     1201 gaacggtccg agcacgacgg ccgacgtcag catcgccagg atcaccacgc tcgcgacggc
     1261 catgcgatgg cgacgaaact ttcgccacgc ctcaaagccg ggcgaaatgc ggcgcggtcg
     1321 cccctcaagc gggacggcaa cgtcaacgat aggagatgcg tgggtcaagc cagccataga
     1381 tgatgtcagc caggaggttg aagacgacga cgagacaggc gaacacgaac gtcaccgcca
     1441 tgatgacagg cgtgtcattg gcgagaatcg ccgtgatcag caacgagccg atccccggga
     1501 tgcggaagat ctgttcggtg acgatggcgc cgccgaacac ggcaggcatt tgcaacgcga
     1561 ccagcgtcac cactggaatc aatgcattgc gaaccacgtg cttgacgatg accttgcctt
     1621 cgtcaaggcc cttcgagcgc gcggtcgtga catagtcaag gcggatgaca tcaagcaccg
     1681 cggagcgcac gtagcgcgta taggatgcgg cttgaaacag gccgaggact gcgaccggca
     1741 tgatggcttg tttgaaattt tcccaatacc agtgccagcc ggtggcgggg atatcgacgc
     1801 gatagacgaa gggcagccag ccgagattga cgctgaagat cagaatcagc agcaagcccg
     1861 tgaagaaagt cggcagcgaa aagccga
//
LOCUS       DASWGM010000726         3570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1583678,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000726 DASWGM010000000
VERSION     DASWGM010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583678"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="VGA15_20365"
     CDS             <1..1831
                     /locus_tag="VGA15_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX9590087.1"
                     /translation="VRAADQAIRIGGALPAQSYLRIEAIIAAAKAGGADAVHPGYGFL
                     AENEDFAQACRDAGLVFIGPSPDAIKAMGNKAGAKTIMQAAGVPCVPGYQGADQSDGA
                     MLTEAKKIGFPVMIKAVAGGGGRGMRQVADAAAFPDLLRSARSEAQGAFGDPTVILER
                     AIANPRHIEIQVFGDRYGHAVHLGERDCSVQRRHQKLIEEAPSPAVSPELRARMGAIA
                     VAAIKSIGYEGAGTLEFLLDKTGEFYFMEMNTRLQVEHPVTEAITGLDLVELQLRVAA
                     GEPLGLRQEEIKFSGHAIEVRLCSEDTAHDFMPQPGRMALWQMPDQLRVEHALQSGSE
                     IPPFYDSMIAKLIGHGATRDEARRRLIHGLEQTAAFGVTTNQAFLAACLRHPAFAAGE
                     ATTAFVGEHRDDLLVRRNDVSADAALAALLLYVTDPHAPPWRKGRTLAATFPIAARLG
                     FDHGIQDLEIVRERDGGYAIQLDGREHRFEIGELGCDTIRFQTNGVMESARFFRDCDR
                     LHVLHRGVTLVVRDLTLAAPAATAATGSDGKVRAAMTGRVVAVLVKQGERVAAGQPVM
                     TLEAMKMEHVHTAPISGTILAIDIAEGEQVTTGKIVVEIEANA"
     gene            complement(1940..2932)
                     /locus_tag="VGA15_20370"
     CDS             complement(1940..2932)
                     /locus_tag="VGA15_20370"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX9590088.1"
                     /translation="MTDVRALLEVTDLVKQYPVRSGILRRQVGTVHAVDGVSFTVGVG
                     ETLGLVGESGCGKSTVARSILRLVEPTGGSIRINGSDITNLGKAELRSHRRSMQIIFQ
                     DPFASLNPRMTAGDIVGEPLSVHGLAAGREKQERIAELFHQVGLRPDQMKNYPHQFSG
                     GQRQRICIARALSLGPSLIVCDEPVSALDVSIQAQVINLLIDLQRKHDFSYLFIAHDL
                     AVVAHISHRVAVMYLGRIVEIAEKTELFANPRHPYTQALLASVPVADPKAKRLVPLID
                     GDVPSPINPPSGCAFHTRCRYVMARCKAETPVLADVGSTHRVACFLNEGTGRKA"
     gene            complement(2929..>3570)
                     /locus_tag="VGA15_20375"
     CDS             complement(2929..>3570)
                     /locus_tag="VGA15_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590089.1"
                     /translation="RLHRPMSRKEATAKAVEMLRLVKIPEAERRAQEYPHQLSGGMRQ
                     RAMIAMALACRPALLIADEPTTALDVTIQAQILALILELQKELGMGLVLITHDLGVVA
                     QTAQRVVVMYAGKKVEEADVETLFASPRHPYTRGLMASIPSLPSLSTNADTRLVEIPG
                     MVPSLTNLPKGCAFAPRCALAMARCREEYPPLQAWGENHLAACWRAAETARSP"
BASE COUNT          657 a         1086 c         1175 g          652 t
ORIGIN      
        1 tgtgcgggcc gccgaccagg ccatccgcat cggcggggcg ctgccggcgc aatcctattt
       61 gcggatcgag gcgatcattg ccgccgcgaa ggctggcgga gccgacgcag ttcatcccgg
      121 ctatggcttt ctggcggaaa atgaggattt cgcgcaagcg tgtcgcgacg ccggtctcgt
      181 gttcatcggg ccatcgcctg atgccatcaa ggcgatgggc aacaaggccg gcgcgaagac
      241 gatcatgcag gccgccggtg tgccctgtgt cccaggatac cagggtgcgg accagagcga
      301 tggcgccatg ctgactgagg cgaaaaagat cgggtttccc gtcatgatca aggccgtcgc
      361 cggcggcggc ggccgcggca tgcggcaggt cgcggacgcc gcggcgtttc ccgatttatt
      421 gcgcagcgcg cgatccgagg cccaaggcgc gttcggcgat ccgaccgtga tactggagcg
      481 cgcgattgcc aatccccgac acatcgaaat ccaggtgttc ggcgatcgct atggccatgc
      541 tgttcacctg ggcgagcgcg attgctcggt gcagcggcgg caccagaaat tgatcgagga
      601 agcgccgtcg ccggcagtgt cgccggaatt gcgggcgcgg atgggcgcca ttgcggtcgc
      661 ggcgatcaag tcgatcggat atgagggtgc cggcacgctg gaattcctgc tcgataaaac
      721 cggcgaattc tatttcatgg aaatgaacac ccgcctgcag gtcgaacatc ccgtcaccga
      781 agcgatcacc gggctcgatc tcgttgaatt gcaattacgt gtcgccgctg gggaaccgct
      841 cggtcttcga caggaggaga taaaattctc cgggcatgca atcgaagtgc ggctgtgctc
      901 ggaggatacc gcccacgatt tcatgccgca gcctggccgg atggcgctgt ggcaaatgcc
      961 ggatcaactg cgcgtcgagc atgcgctgca atccggttcc gagattccgc cgttctatga
     1021 ttcgatgatt gcgaaactca tcggccatgg cgcaacccgc gatgaggcgc ggcgcaggct
     1081 cattcatggc cttgagcaga ccgctgcatt cggcgtgacg accaatcagg cgtttctcgc
     1141 ggcgtgcctg cggcatcccg cgttcgcggc cggcgaagcg acgacggctt ttgtcggcga
     1201 gcatcgcgac gatctgctgg tgcggcgcaa cgacgtgtcc gctgatgccg cgctcgcggc
     1261 gttgttgctc tacgttaccg accctcatgc gccgccgtgg cggaaaggac gaacgctggc
     1321 agcgacattc ccgatcgctg ctcgtctagg gtttgatcat ggcattcagg atctcgaaat
     1381 cgtgcgcgag cgcgatggtg gttacgccat acagctcgat ggccgcgagc accggttcga
     1441 gatcggtgag cttggctgcg atacgatccg ttttcagacc aacggcgtga tggaatcggc
     1501 aagatttttc cgggactgcg accggctcca tgtcctgcac cggggcgtca cgcttgtcgt
     1561 ccgcgacctg acgttggcgg cgccagccgc gacggcggca accggcagcg acggcaaggt
     1621 ccgcgcggcg atgaccggac gcgtcgtcgc ggttctggtc aagcagggcg agcgcgtcgc
     1681 agccggtcag ccggtgatga cgctggaagc catgaagatg gagcacgtgc atactgcgcc
     1741 gatctcaggc acgattttgg ccatcgatat cgcggaaggc gagcaggtga cgacggggaa
     1801 gattgtcgtc gagatcgaag cgaacgcgta accgtgagat agtgttcgtc gtgcccaggc
     1861 ttgtcccggg catccacgcc ttaaaagcat agccacaaga aagacgtgga tggccgggac
     1921 aagcccggcc atgacggcgc taggccttcc gccccgttcc ctcattgaga aaacaagcca
     1981 cccgatgcgt cgatccgaca tccgccagca ccggcgtctc tgccttgcac cgcgccatca
     2041 cataacgaca gcgggtgtgg aaggcgcagc ctgacggcgg attgatcggg ctcggcacat
     2101 cgccatcgat caacggcacg aggcgttttg ccttgggatc ggcgaccgga accgaggcca
     2161 gcaaagcctg cgtataggga tgacggggat ttgcgaacag ctcggtcttc tccgcaatct
     2221 cgacaatgcg gccgagatac atcacggcga cgcggtggct gatatgggcc acgacggcaa
     2281 gatcatgcgc gatgaacaga tacgaaaaat cgtgcttgcg ctgcaggtcg atcaacagat
     2341 tgatcacctg ggcctggatc gagacgtcga gcgccgatac cggctcgtcg cacacgatca
     2401 ggctcggccc gagcgacagc gcgcgtgcga tgcagatgcg ctgccgctgc ccgccggaga
     2461 actggtgggg ataatttttc atttggtccg ggcggagccc gacctgatgg aataattcgg
     2521 cgatccgttc ctgcttttcg cggccggccg ccagcccgtg gacgctgagc ggctcaccga
     2581 cgatatcgcc agccgtcatg cgtggattga gcgaggcaaa cgggtcctgg aagatgatct
     2641 gcatcgagcg ccgatgcgaa cgcagttcgg cttttccgag attggtaatg tcgctgccgt
     2701 tgatccggat gctgccgccg gtcggctcga ccaggcgcag gatgctccgc gcgaccgtcg
     2761 atttgccgca accggattcg ccgacaagcc cgagcgtttc tccgacgcca accgtaaaac
     2821 tgacaccgtc caccgcgtgc acggtgccga cttggcggcg aaggattccg ctgcgcaccg
     2881 gatattgttt gacgaggtcc gtcacctcga gcagagcgcg cacatcggtc atggtgatct
     2941 cgcggtttcc gccgcgcgcc agcacgccgc caaatggttc tcgccccagg cctgcagggg
     3001 cggatattcc tcgcggcagc gggccattgc gagtgcgcat ctcggagcga aggcgcagcc
     3061 tttcggcaga ttggtcaatg agggcaccat accgggaatc tcgaccagcc gcgtatcggc
     3121 gttggtgctg agcgagggca gggatggaat cgaagccatc agaccgcggg tgtagggatg
     3181 ccttgggctc gcgaacaagg tttcgacgtc ggcctcttcg accttcttgc cggcatacat
     3241 gacgacgacg cgctgcgcgg tctgcgcgac cacgccgaga tcatgcgtga tcaggacaag
     3301 tcccataccc agttctttct gcagctccag tatcaacgcc aggatctggg cctgtatggt
     3361 gacgtcaagc gcggtggtcg gctcgtcggc gatcaacagc gccggccggc aggccagcgc
     3421 catcgcgatc atcgcgcgct ggcgcattcc gcccgagagc tgatggggat attcctgggc
     3481 gcgccgctcc gcttccggga ttttgacgag gcgcagcatc tcgacggcct tggccgttgc
     3541 ctctttccgg ctcatcggcc gatgcaggcg
//
LOCUS       DASWGM010000727         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_197839,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000727 DASWGM010000000
VERSION     DASWGM010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197839"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /gene="merA"
                     /locus_tag="VGA15_20380"
     CDS             <1..1372
                     /gene="merA"
                     /locus_tag="VGA15_20380"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104009825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HEX9590090.1"
                     /translation="GAGSAGFSASIAAAEQGAQVALIGSGTIGGTCVNIGCVPSKTLI
                     RAAETLHNARVAARFAGIAAEAELTDWSATIRQKDALVSELRQAKYIDLLPAYNSIVY
                     REGPARLVDGGVEVGDAHISAGKIIIATGARPAVPLIPGIETVPYLTSTTALDLEVLP
                     RSLLVVGGGYIGAELAQLFARAGVKVTLVCRSRLLPEAEPEIGAALTGYFQDEGIAVV
                     AGVTYRAIHKTETGIALDVSREGQDTTIDADQVLIATGRTPNIEGLGLIDRGVAVSPK
                     GGIVVDDRMRTSRTCVYAAGDVTGRDQFVYMAAYGAKIAAKNALNGDSLRYDNSAMPG
                     IVFTDPQVASVGLTEAGARAAGYAVRVSTIGLDQVPRALAARDTRGLIKLVADAGSGR
                     LLGAHILAPEGADSIQTAALVITHGLTVQQLADTIFPYLTTVEGLKLAALSFDKEVAK
                     LSCCAG"
     gene            complement(1500..1730)
                     /gene="nrtS"
                     /locus_tag="VGA15_20385"
     CDS             complement(1500..1730)
                     /gene="nrtS"
                     /locus_tag="VGA15_20385"
                     /inference="COORDINATES: protein motif:HMM:NF038050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NrtS"
                     /protein_id="HEX9590091.1"
                     /translation="MLTLRLACRCALSDGVPRRSFFVALVVGTVLNLINQGDALFGAA
                     PINWLKVILTYFVPYAVCTYGAVSFQLRKMRP"
     gene            complement(1750..2448)
                     /locus_tag="VGA15_20390"
     CDS             complement(1750..2448)
                     /locus_tag="VGA15_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX9590092.1"
                     /translation="MATNSSGAVRACVFDAYGTLFDFASAARRCRDVLGDSFDRLTAL
                     WREKQLQYTWLRAAQGRHADFWQVTGDALDFSLETLLLDQPGLRDRLMELYLTLDTFP
                     EVPDVLRQLKAAGLKTAILSNGTPAMLDSAVKNARIDGLLDIVLSVEEVGVYKPDPRV
                     YQLAVDQLGIPASQISFQSSNAWDAYAASAFGMKVAWCNRYRQRKERLPGRPDYEIET
                     LAELPRIVGAVREM"
     gene            3321..3755
                     /locus_tag="VGA15_20395"
     CDS             3321..3755
                     /locus_tag="VGA15_20395"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX9590093.1"
                     /translation="MRLFRLEGGTIDLVSLRGKPILLNFWASWCAACRTELPILERQY
                     GAAWRANLHVVAVSEDGGSRQAVERFVKDLELRTLPIYLDPNGSVAYSDSANSRNAPF
                     ALYGMPITYLIASSGWVVGYMPGAADWSSAAASDLIEFLHNM"
     gene            3945..4301
                     /locus_tag="VGA15_20400"
     CDS             3945..4301
                     /locus_tag="VGA15_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9590094.1"
                     /translation="MLVAATLLASSLVAWAGQPFDAKAFQSAQAAEKVILVDVTASWC
                     PTCRQQRPIVEQIEREKPDLVVLEADFDTAKDVLKQFRVQYQSTLIVFRGSKEVARST
                     GETDPSLIRALIAKAF"
     gene            4304..5029
                     /locus_tag="VGA15_20405"
     CDS             4304..5029
                     /locus_tag="VGA15_20405"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX9590095.1"
                     /translation="MPGVGNLALGYAAGALSTLSPCVLPILPIILFGAIERHALGPLA
                     LAAGLSASFAGVGTVIASAGFSIGLDPSALRLAIAALMTGLGIILLVPSLQGRLATVA
                     MPVATKGQTLLDRIQPSGIGGQFVLGALLGVIWSPCSGPTLGAAIGLAAQGDNVGAAA
                     ATMISFGLGAVTPILALTYGSRQAISARRDWLARMSRTARPLLGGAFVGLGAFVLTGL
                     DRITEAILTRAMPDWLPTVTTRL"
     gene            complement(5079..>5326)
                     /locus_tag="VGA15_20410"
     CDS             complement(5079..>5326)
                     /locus_tag="VGA15_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590096.1"
                     /translation="PAKAVPIDAMFRRLAISGIGSLEKVTSAVGIAGREQAANNECVR
                     KRAFRASMRPRPVSGTVGFTDVGRTVEGTASVWGRPQ"
BASE COUNT         1004 a         1678 c         1582 g         1062 t
ORIGIN      
        1 cggcgccggc tcggccggct tctcggcctc gatcgcagcc gccgagcaag gcgctcaggt
       61 ggcgctgatc ggcagcggca ccatcggggg cacctgcgtc aatatcggct gcgtgccgtc
      121 gaagaccctg atccgcgccg cagagacgct tcacaacgcc cgcgtggcgg cacgtttcgc
      181 cggcatcgcg gcggaagccg aactgaccga ctggagtgcg accattcgtc agaaagacgc
      241 actcgtttcc gagctgcgcc aggccaaata tatcgacctg ctgccggcct ataacagcat
      301 cgtctatcgc gaaggcccgg cgcgccttgt ggacggtggc gtcgaggtgg gtgacgctca
      361 catttccgcc ggcaagatca tcattgcgac cggcgcgcgg cctgcggtgc ccttgattcc
      421 cggcatcgag acggtgccgt atttgaccag cacgacagcg ctcgatcttg aggtgctgcc
      481 gcgatcgctg ctcgttgtcg gcggcggcta catcggagcg gagcttgccc agctgttcgc
      541 ccgcgccggc gtcaaggtaa cgctggtgtg ccggtcccgc ctgctccccg aagccgagcc
      601 cgagatcggc gcggcgctga ccggatattt ccaggacgaa gggatcgccg tggtggccgg
      661 cgttacctac cgcgcgatcc acaaaaccga aaccggcatt gcccttgacg tttcccgcga
      721 gggccaggac acgaccatcg acgccgatca ggtgcttatc gccacgggtc ggacgcccaa
      781 catcgaaggt cttgggctta tcgatcgtgg cgtcgcggtc tcgccgaagg gaggcatcgt
      841 cgtcgatgat cgcatgcgca cgagccgcac ctgcgtctat gccgcgggcg acgtcaccgg
      901 ccgcgaccag tttgtctaca tggccgccta tggcgccaag atcgcggcga agaatgccct
      961 caacggcgac agcctacgct atgacaacag cgccatgccc ggcatcgtgt tcaccgaccc
     1021 acaggtggcg agcgtcggcc taaccgaggc cggcgcacgc gccgccggat atgcggttcg
     1081 cgtctcgacg atcggtctcg atcaggtgcc gcgcgcactt gccgcgcgcg acacccgtgg
     1141 gctcatcaag ctcgtggccg acgcaggcag cggccggctg ctgggtgcgc atatcctcgc
     1201 gccggaggga gccgatagca ttcagaccgc ggcgctggtg atcacccatg gcctgacggt
     1261 tcaacagctt gccgacacca tcttccccta tctgaccacg gtggagggtc tgaagcttgc
     1321 ggcattgtcc ttcgataagg aggtggcgaa actgtcgtgc tgtgccgggt gagcattcgt
     1381 ctgcgcattg ccggtcacac gctcaaatcg tcgatcggtt attgactccg taccaatgtg
     1441 cgggccgctg tctatgccgg acggctgaag ccatagcagc aagctgtcgt ggtgcgtacc
     1501 tagggacgca tcttgcggag ctgaaacgaa accgcgccat aggtacaaac cgcatacggc
     1561 acgaagtaag tcagaatgac cttgagccaa ttgatcggcg ccgcgccaaa cagcgcatcg
     1621 ccctgattga tgaggttcaa caccgttccc acaacaaggg cgacaaaaaa cgaacggcga
     1681 ggcacgccat cagagagcgc gcaccggcac gcgagcctca atgtcaacat gtgtttgcca
     1741 agcttgcgtt cacatctctc gaaccgcacc gacgattctc ggtaattcgg caagcgtttc
     1801 gatttcgtaa tcgggtctgc ccggcagacg ctccttgcgc tgtcgatacc gattgcacca
     1861 ggcgactttc attccaaagg ccgacgcggc atacgcatcc cacgcattcg aggattgaaa
     1921 cgaaatttgc gaagctggaa tgcccagttg gtcgaccgcg agctgataga ctcgaggatc
     1981 cggtttgtag acgccaacct cctcgaccga caatacgata tcgagcaaac catcgatccg
     2041 ggcattcttt acagccgagt cgagcatcgc aggcgttccg ttcgacagga tcgcggtctt
     2101 caatcctgcg gccttcaatt gccggaggac gtcgggcacc tccgggaagg tgtcgagagt
     2161 caaatagagc tccatcaaac gatcgcggag cccaggctga tcgagcagca gggtctccaa
     2221 cgagaaatcc aaggcgtcac ccgtcacctg ccagaaatcg gcatgccgtc cttgtgcggc
     2281 acgaagccag gtatattgga gctgcttttc gcgccaaagg gcggtcagcc tgtcgaagct
     2341 atcaccaagc acatcgcggc agcggcgcgc cgcggacgca aaatcgaata gcgtgccata
     2401 ggcatcgaag acacaagccc tcacggcacc cgacgagtta gtggccatgg ccttctctcc
     2461 gtcggcctgc agactatcga attttgggcg ccaagacgag gcagtgcaat ttcatctttg
     2521 aaccagttcg cttttgccgg gccgaagtta cgcggtgccg acgattcttg tggagggctg
     2581 cccctccttc gaggtcggct gccgaacatt ctggcgccgt cagggttggt cggtatggca
     2641 ccaccatttt ctgtgatccg gtaacctttc ccacccaagg aacgaatatt caggagtgaa
     2701 atgcctgcac aagggccgag tttcggaaca ggaggagcga aaagatgcga acgacggcaa
     2761 tgcgctccat ccgcataact catcgaccaa gcggcgcgat cattgccgag ggaccgctcg
     2821 gattttgggg tattacgccg tttgaaggga atctctacat cagccgaaaa tgcttgaaga
     2881 cgaactgcct gcgaccgaat tgggtaccgg ggctctgcat ctacaaattc ctttacgttt
     2941 ggctcgatct gcgactcccc gacggaacgc gagagccctt tgtcggatgg atgtactggc
     3001 tgccaaaccc gctcctaccc tttatcgcgt ttcggccggc cgttcctcgc tcttcaccga
     3061 cgctacagat tgacgagata accggttgac gcgaacgcat atcgagcctg ccgatggtta
     3121 gcagctgatc agacactgct ccggttcgcc ccggcgtcaa agccccgacg cggcgggata
     3181 tcgtcctctc gggactagtt gggacgcttg cgtccctcgg aagcacggcg atcgcgtcac
     3241 cgcaagcggt cgatgtcccc agcttccaat ccggcagata tcagttcacg atccttcggc
     3301 cgcagctaga gctcccatcg atcaggctgt ttcgtttgga gggtggcacc atcgatctcg
     3361 tgtcgcttcg gggtaagccg atccttttga atttttgggc atcgtggtgc gccgcctgcc
     3421 ggacagaatt acccattctt gagcgacaat acggcgcagc ttggcgcgcc aacctgcatg
     3481 tcgtcgccgt ttcggaggac gggggcagcc gacaggcggt tgagcgcttc gtcaaagacc
     3541 tcgagctgcg aacccttccg atctatctgg accccaacgg ctctgtcgca tattcggaca
     3601 gcgccaatag caggaacgcg ccgttcgccc tttacgggat gcccatcacc tatctcatcg
     3661 caagctccgg atgggtcgtg ggctacatgc cgggcgccgc agattggtcg tcggctgctg
     3721 ccagcgacct catcgaattc ctccacaaca tgtgaggcag catggcgccg tccaaggtgc
     3781 tgcggcgttt tcgtctcgat gcgccggctc cttctcgttc gagccggtgt ttttgtgtga
     3841 ccgggtaacc ttttcggtgt tccgagcgaa ccgctccgag aacctcgagg ttcgcggcat
     3901 gcaacggaga ccgaaagatc atgcattcca aactctgtaa ggttttgctg gtcgcggcga
     3961 cgctccttgc gtcgtcgctt gtcgcttggg cggggcagcc gttcgatgcc aaggcgtttc
     4021 aatccgccca ggccgcggaa aaggtgatcc tcgtcgacgt caccgcatcc tggtgtccga
     4081 cctgtaggca gcaacggccg atcgttgagc agatcgagcg agaaaagccg gatttggtcg
     4141 tcctggaagc cgattttgac actgcaaaag acgtcttgaa gcagtttcgc gttcagtacc
     4201 agagcacgct catcgtcttc cgaggtagca aggaggtcgc gcggtcgacc ggcgaaaccg
     4261 acccgagcct tatccgcgcg ctgatcgcga aggcattctg actatgcccg gtgttggcaa
     4321 tttggcgctc ggctacgcag ccggcgcgtt atcgacgttg tccccttgtg tgctgccgat
     4381 actgccgatc atcttgttcg gagcgatcga gcgacatgca ttgggaccct tggctctggc
     4441 cgcggggcta tcggcttctt ttgccggggt cggaactgtc atcgcatcgg ccggcttcag
     4501 catcggactt gatccatccg cgttgcgcct cgcgatcgcg gccctgatga caggcctggg
     4561 aatcatcctt ctcgtcccgt cgcttcaagg cagattagcg accgtcgcga tgcccgtcgc
     4621 gacaaaagga caaaccctgc ttgaccgaat tcagccgtct ggaattggcg gccagttcgt
     4681 ccttggcgcc ctgctcgggg tgatctggtc tccttgctca ggtccgacgc ttggggcagc
     4741 aatcggactc gccgcgcaag gcgacaatgt cggcgccgcg gcggcaacca tgatcagttt
     4801 cggtctcggt gcggtaacgc cgatcctcgc gctcacttac ggctcgagac aagccatctc
     4861 tgcgcggcgc gattggctgg cgcgaatgtc gcgcaccgcc agacctttgc tgggcggggc
     4921 cttcgttggt ctcggcgcct ttgtcctcac cggcctcgac aggatcaccg aggccatctt
     4981 gacgcgcgct atgcccgact ggctgccgac cgtgacgacg cggttgtgaa gcgcgtcaat
     5041 ttttcaaacc aaagaaatgc atggccacgc tcaagacatc actgcggtct cccccagact
     5101 gacgccgttc cttctaccgt tcgcccgaca tccgtaaatc ccaccgttcc gctgacaggt
     5161 cgcggccgca tcgaggcgcg gaaggccctc ttccgaacgc attcgttgtt cgcggcctgc
     5221 tcgcgtccgg caatccccac cgcgctcgtc actttttcca acgatccgat gccgctaatc
     5281 gccaggcgcc ggaacatagc gtcaattggt acggctttcg ctggag
//
LOCUS       DASWGM010000728         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_351672,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000728 DASWGM010000000
VERSION     DASWGM010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351672"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VGA15_20415"
     CDS             complement(<1..1357)
                     /locus_tag="VGA15_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590097.1"
                     /translation="MSDLSSTSGQAALKRQLGVNPNVLMPMLWLAATVAIALPAINGG
                     VFDAMSTDDAMRLVQVRDWIGGQGWFDLFQHRLDPPGASMHWSRVVDVPLAALILLLR
                     PLIGTHGAETVTLFLWPLLLFAAALGLVAAIAREMSGSVTTSQITAVVLAVLSAPALI
                     HFRPGAIDHHNAQIVLLLALVLLTSQIEQSAVKAALGGLVASLSLAIGIEMLPAIAAI
                     GLAVFGLFIWRGASVSRQIGAFGAALAASSLLLAPALLPLPSLALPVCDAFGGPVLLL
                     VAGGGISLMIMAGIDRLHSTLRLRLATGAASAIALVGAFLSLFAGCLASPYAQLDPLV
                     TSFWVDKVVESISLATMLQLAPQKVPGFYGFPLMTMGFAAAALIRSNPLGRFRWILGI
                     MTLAALIGLSVWELRGAAAASMVAAPMFAASLAILWPTLATGRSLILLALAVSPASFA
                     AL"
     gene            complement(1496..2512)
                     /locus_tag="VGA15_20420"
     CDS             complement(1496..2512)
                     /locus_tag="VGA15_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590098.1"
                     /translation="MLLAHIGPTLEGHPEAVLFHRLFNVLLVAVGLAALMAVGLVAKL
                     PRLTLYAYIVPIAGIPVLAPLAGAINNDNAAFAGGAIATLATLQLLSTGSRAWLIAAL
                     GGIILASWAKFTALLLVGGLVGGVLLWLLWRGRLPSRWVGLIAIAALLAAAPYVALFV
                     QYRSPTPRTPGQIAMIKSGALAVGWDSAERMTPTSYAVHFVSEFVLEWMPTLKPRNAL
                     NYAALTIPITAALCAFVGFLVAVQRIAHEKAGPLDIMVATGMLAFAATFLLHGIFSYG
                     LHTEFGWQTTAYPRYYLPLAAIVPLAGLSLLGAIKPPSARAILLVFLIAGPLAFRLLG
                     APLG"
     gene            3019..3750
                     /locus_tag="VGA15_20425"
     CDS             3019..3750
                     /locus_tag="VGA15_20425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEX9590099.1"
                     /translation="MARIGTRAEVWFSNVVASFARYVAERTLPESVPMLMRRDAVLDS
                     FNYAKENMQDAYSFLDRFDGLRLSIEEAKRRFPARKLVLEFGVYKAGMINYQARNFPE
                     LNFVGFDSFEGLQEQWSGMAPKRTFDLGGRLPRVRRNVGLVKGWFAESGPRWKKENPA
                     SGIPLLVHVDCDTYAATVDVLEICSDYVEHGLVIHFDDYFAFPDWRNAGFKALKEIAE
                     RRRWRLTYLSYGTKEVAVLAEMHVG"
     gene            3890..>5205
                     /locus_tag="VGA15_20430"
     CDS             3890..>5205
                     /locus_tag="VGA15_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9590100.1"
                     /translation="MTTTDADIFVIGAGPAGLTTAYCLTKETPSVIVIEKDPVYVGGI
                     SRTVRHGNFLFDIGGHRFFSKAKEVVDLWLEILPDDFITRPRLSRIYYGGKFYSYPLS
                     AFQALRNLGIFTSAACMLSYAYAKAFPIASPRSFHDWIRNQFGERLFQIFFKTYTEKV
                     WGMSCDEISADWAAQRIKGLDLRVAVVNALKHSFGRKPKAVAVGDDVVKTLIGTFQYP
                     RKGPGMMWEAAAAKIRERGGRILMGRELVELAYDEVHKRWRIGVMTSDGKRESYTARH
                     VVSSAPVRELVQKLSPKPISLLHARALRYRDFLTVALMTDKTDVFADNWIYIHDPSVK
                     VGRVQNFGSWSPEMAPAGMTCLGLEYFCFEGDGLWTMSDEELIALATHEVVKVGLLAA
                     TDVIDGCVVRQPKAYPVYDDSYRDHMAMIRRDLEQSYPSLHLIGRNG"
BASE COUNT         1150 a         1555 c         1580 g          920 t
ORIGIN      
        1 ccaaggctgc aaagctcgcc ggcgacacgg cgagcgccaa aaggatcagg cttcgtccgg
       61 tggctagcgt tggccacaga atggccaggc tcgcggcaaa catcggagcg gccaccatcg
      121 atgccgccgc cgcgccccgc agttcccaaa cgctaagccc gatcagcgcg gccagcgtca
      181 tgatgccaag gatccagcgg aaccgtccaa gcggattgga ccggatcagc gccgccgcgg
      241 cgaagcccat cgtcatcaat ggaaacccgt agaagccagg gactttttgc ggcgcgagct
      301 gcagcatcgt cgcaagtgaa atcgattcga cgaccttgtc gacccaaaat gatgtcacga
      361 gcggatcgag ctgggcgtaa ggcgacgcaa ggcatcccgc aaacagggag aggaacgcgc
      421 caaccaaagc gattgctgac gctgctcccg tagccaggcg caggcgcagc gtagagtgga
      481 ggcgatcaat tccagccatg atcatcaggc tgatgccgcc gccggcgacc agcagcagca
      541 ccggcccgcc gaaggcgtcg cagaccggca acgccagcga aggcagtgga agcaatgccg
      601 gtgcgagcag gagcgatgat gccgccagcg ccgccccgaa ggcgccgatc tgtcgcgaga
      661 cggatgcacc gcgccagatg aaaaggccaa atacggcgag acctatcgct gcgatggcgg
      721 gcagcatttc aatgccgatc gccagcgata gcgatgccac caggccgccc agcgcggcct
      781 tgaccgcact ctgctcgatc tgtgacgtca gcaagaccaa agcgagcaac aaaacgatct
      841 gcgcgttgtg gtgatcaatc gcacctggcc ggaaatggat cagcgccggc gcggatagca
      901 ccgccagcac cactgccgtg atctgcgagg tagtgacact gccactcatt tcccgggcaa
      961 ttgccgcgac cagcccgagg gctgctgcaa acagcagcaa gggccaaaga aatagcgtca
     1021 cggtttctgc gccgtgcgtg ccaatcagcg gcctcagcag caggattaac gcggccaggg
     1081 gcacgtcgac gacgcgcgac cagtgcatcg acgcgccagg cggatccaat cgatgttgga
     1141 agaggtcaaa ccagccttgc ccaccgatcc agtcgcgaac ctggacgagc cgcatggcat
     1201 cgtccgtaga catggcatcg aagacgccgc cgttgatcgc gggcaacgca atggcgaccg
     1261 ttgccgccag ccacagcatc ggcatcaaga cgttggggtt cacgccgagt tgccgtttca
     1321 gcgccgcctg gcctgaggtc gaactgagat cggacatggt ttcctcatac ctatcgagct
     1381 agccctacgg ccgttacgga tcaattgacg gcgagcatgc gacgcggctg aaatgcttgc
     1441 cgcggcggcc gctctcgtcg gagctaaaaa ggccgcgaac tgtcaacccg aaaacctatc
     1501 ccaatggcgc acccaacaag cggaaggcca gggggccggc aatcaggaag acaagcagta
     1561 tggcgcgggc ggacggcggt ttgattgcgc ccagcaatga caaccccgcg agtggaacga
     1621 tggccgccag cggtaaatag tagcgcgggt aggcggtcgt ctgccagccg aactcggtat
     1681 gcagcccata gctgaagatg ccgtggagaa ggaacgttgc ggcaaaagcc agcatgcccg
     1741 tcgcaaccat aatatcgaga gggccggctt tttcgtgcgc gattcgctgg accgccacca
     1801 aaaacccgac gaaggcgcac agcgccgcgg tgatcgggat ggtcagcgcc gcataattca
     1861 gcgcattgcg tggcttcaaa gtcggcatcc attccaggac aaattccgag acgaaatgga
     1921 ccgcatagga cgtaggcgtc attcgctcgg cgctatccca gccgaccgcc agcgcgcccg
     1981 acttgatcat cgcgatctgt cccggtgtcc ttggcgtcgg actgcgatac tgcacgaaca
     2041 acgcgacata gggagcggcc gccagcagcg cggcgatagc gatcagcccc acccatcggg
     2101 acggcaagcg cccgcgccac aacaaccaga gcaacacgcc gccgaccaat ccgccgacca
     2161 gcagcagcgc ggtgaacttc gcccaggacg cgaggatgat gccgccgagc gcagcgatga
     2221 gccacgcccg gcttccggtg ctcaggagct gtaacgtagc cagcgtggcg attgccccac
     2281 cggcgaatgc ggcgttgtcg ttgttaatgg cgcccgcaag cggcgccagc acggggatgc
     2341 cggcgatcgg aacaatatac gcatagagcg tgagccgcgg cagcttcgcg acgagcccga
     2401 ccgccatcag agccgcgagc ccgaccgcaa ccagcaacac attgaatagc cgatggaaca
     2461 ggaccgcctc gggatggcct tccagggtgg gaccaatgtg ggccagcaac acataatacg
     2521 gcgaggggtg atcgagatag tttgcttcgc tggtaaagcc gaagctcgat gggtcgagca
     2581 tccgcatatc ctcaaaggcg ggccaggtct cgccggagtg ttggagatgc gcaacatagg
     2641 atacgtgtgc cacttcgtcg aacccaagcg tcacatcctt gcgtatcgca ccaagtgtcg
     2701 cggcgacgac gaaaagcaaa agcacaatgg cgcaaacgag ccagttggct tcggccgcga
     2761 ccgcagtttg atcttgcgag gatgtattga aattacttgg agcatgcatc gtgccagtgt
     2821 gcactggcac cccttaaaca agtcctatgc catgcgacag cccacggtga gccgcgatac
     2881 caaatttagg tctatattag gcagacccga gcagaaagta gcaacgattt gagcccataa
     2941 tccccgtttg agccaagccg ccggacggcc cggaacaggc cacggcttct gcaagatgat
     3001 gccgaggata aatctgatat ggcgcgcata ggtactcgag cggaagtctg gttttccaac
     3061 gtcgttgcct cgttcgcgcg ctacgtcgcg gaaaggacgc tgcccgaatc cgttcccatg
     3121 ttgatgcgcc gcgatgccgt cctggactcc ttcaactacg ccaaagaaaa tatgcaagat
     3181 gcgtattcgt ttctggatcg attcgacggt ctcagacttt cgatcgaaga ggcaaagcga
     3241 agatttcctg cccgcaaact tgtactggag tttggcgtct acaaagctgg aatgattaac
     3301 taccaggcca ggaattttcc cgagttgaat tttgtcgggt tcgacagttt tgaaggtttg
     3361 caggaacaat ggagcggcat ggctccgaaa agaacctttg accttggcgg caggctgccg
     3421 agggttcgac gcaatgtcgg gttggtgaag ggatggttcg cggagtcagg gccccgttgg
     3481 aaaaaggaaa atcctgcgtc cggcatcccg ctattggtgc acgttgactg tgacacctac
     3541 gccgcaacgg tcgacgtgct ggaaatctgt tctgactacg tcgaacatgg tctcgttatt
     3601 cattttgacg attactttgc gtttcccgat tggagaaacg ccgggttcaa ggcgctaaag
     3661 gaaatcgcgg aaagacgacg ttggcgtctg acctatttgt cctacgggac aaaagaggtg
     3721 gcggttctgg cggaaatgca tgttggctga cgtggtcgcg cgacaaattg ctacgcgcgc
     3781 attgatgctt cattaacgct tcaagcgacg cttctgcgag cggcgataag cccaacctta
     3841 acgaagttct gcgcaaatgc ggcccgaaac ccgagggatc gcgccgcaca tgaccaccac
     3901 agatgcagac attttcgtga tcggcgccgg ccccgccggc ctcaccacgg cttactgcct
     3961 gaccaaggag acgccgtcgg tgatcgtgat cgagaaagac ccggtctatg tcggcggcat
     4021 cagccgcacc gtgcggcacg ggaatttcct gttcgatatt ggcggccatc ggtttttctc
     4081 caaggccaag gaagtcgtcg acctctggct cgagatcctg cctgacgact tcatcacgcg
     4141 gccgcggcta tcgcggatct attatggcgg aaaattctat tcctatccgt tgagcgcgtt
     4201 ccaggcgctg cgcaatctcg ggatattcac cagcgccgcc tgcatgctgt cctatgccta
     4261 tgccaaggca ttcccgatcg cgtcgcctcg cagcttccat gactggattc gcaatcagtt
     4321 cggcgagcgc ctgtttcaga ttttcttcaa gacctacacc gagaaggtgt ggggcatgtc
     4381 ctgcgacgaa atctccgccg actgggcggc gcagcgtatc aagggactgg atcttcgcgt
     4441 cgcagtcgtg aacgcgctga aacattcgtt cgggcgcaag ccaaaagccg ttgcggtcgg
     4501 cgacgatgtc gtgaagacgt tgatcgggac gttccaatat ccgcgcaagg gtcccggcat
     4561 gatgtgggaa gccgctgcag ccaagatccg ggagcgcggc ggccgaattc tgatgggtcg
     4621 cgagctggtc gaactcgctt atgatgaggt ccacaagcgc tggcgcatcg gggtcatgac
     4681 atccgacgga aaacgcgaga gttatacggc tcgccacgtc gtgagctccg caccggtgcg
     4741 cgagctggtg cagaaattgt cgccgaaacc catttcgctg ctgcacgcgc gcgcgctgcg
     4801 ctaccgtgat ttcctcaccg tcgcgctcat gacggacaag acggacgtgt ttgccgacaa
     4861 ctggatctat atccatgatc ccagtgtgaa ggtcggtcgc gtgcagaatt tcggttcgtg
     4921 gtcccccgaa atggcgccgg ccggcatgac ctgcctcggc ctcgaatatt tctgcttcga
     4981 gggcgacggc ctgtggacga tgtccgacga ggagcttatc gcgctcgcca cgcatgaggt
     5041 cgtcaaggtc ggtttgctcg ccgccaccga tgtgatcgac ggctgcgtgg tgcgccaacc
     5101 caaggcctat cccgtctatg acgattccta tcgcgaccat atggcgatga tccggcgcga
     5161 cctcgagcaa agctatccga gcctgcatct gatcggccga aacgg
//
LOCUS       DASWGM010000729         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1033324,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000729 DASWGM010000000
VERSION     DASWGM010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033324"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGA15_20435"
     CDS             complement(<1..398)
                     /locus_tag="VGA15_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX9590101.1"
                     /translation="MSAVQETVDRVRQIDVDQYRYGFETLIESDKAPKGLSEDTVRFI
                     SAKKNEPAWMLEWRLEAYRRWLTMTEPKWARVNYPKIDYQDLYYYSAPKPKKTIGSLD
                     EVDPEILKTYEKLGIPLREVEVLEGVVRPEG"
     gene            complement(481..1647)
                     /locus_tag="VGA15_20440"
     CDS             complement(481..1647)
                     /locus_tag="VGA15_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX9590102.1"
                     /translation="MPDRVYLDWNATTPLRPEAREAMAAAWDLCGNPSSVHAEGRQAR
                     RLVEEARAAVASAVGALPRNVVFTSGGTEANALALTPGLRRGSGLPVERLLVSAIEHA
                     SVLAGGRFPAEAISMIGVTSAGLLDLDHLRTKLEAGPPALVSVMLANNETGAVQPVAQ
                     VAEIVHAAGGLLHVDAIQALGKISFDIKALNADLVTVSGHKIGGPKGVGALALAEGVR
                     GLEPLLRGGGQELGHRAGTENVAGIAGFGAAAKAAMGTREGDTIRLESLRNRLERGLR
                     ETTGAIVFSGDVPRLPNTTLFTVPGLKAETAVIGFDLEGIAVSSGSACSSGKVQPSHV
                     LKAMGFGPEIAEGAVRLSQGWSTSSADIDRCLKAWRKLAGTLLKGSDETLLERF"
     gene            complement(1681..2913)
                     /locus_tag="VGA15_20445"
     CDS             complement(1681..2913)
                     /locus_tag="VGA15_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX9590103.1"
                     /translation="MKRQTVSHSPHFAAQGQERRVDLDWVRIGAFGLLIFYHVGMLYV
                     SWGFHIKSAHRITALEPLMLVLNPWRLALLFLVSGAATRFMLGKYPIGPLLRSRSVRL
                     LIPLIFGMLVIVPPQAYDQIVESLGYPAGFADFYARHYFAFGPQFCRPGPCILLPTWN
                     HLWFVAYLWIYTMALGAVWVAVPGLVDWMERRLVPALAGVRLLIVRSVTFAAYRLAVL
                     PNFPSTHALFGDWYNHAVYASVFLLGFLLARAEAFWDAIESQRRLALSLAAALFLSFL
                     VLRWTRESATPPSLLLKLYGGIAYGCYQWLCIVAVLGFARRWLTADSGVRRYLTDAIF
                     PYYIVHQTAIIMIAHELHGRDLPVWLEASIVISGTLAVCVLTYETIRRIAILRPLFGL
                     RVAAFEPTPVQPTAQPAQ"
     gene            2944..3567
                     /locus_tag="VGA15_20450"
     CDS             2944..3567
                     /locus_tag="VGA15_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590104.1"
                     /translation="MSDVKNAKGGPGVEPVWDQKPPAWDEVRPRGTSGGLGGISGADW
                     LLYGAVAAVALGIGLVNALSKAQDVAWRGGVYDARTQLFWELSSVVTIILVAPVLFVA
                     VRRMRHVRGWPLRIALAVAAILAFSSLHIAGMVGLRKLAMLLVGSAYDFHFSAATRSG
                     CATVQRASASNPARFSGSARPEITSNTALPTASITSSAAPSPPRKRG"
     gene            3474..3782
                     /locus_tag="VGA15_20455"
     CDS             3474..3782
                     /locus_tag="VGA15_20455"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX9590105.1"
                     /translation="MSSAGNYVEYSLADGVNHLVRGTLAAEEARLTPFNIVRVHRTRL
                     VNLTRVRGLKTGANGDFELTLDTGQTISGSRRYRGAVASIEELAANPASVLTNNGAHS
                     "
BASE COUNT          798 a         1300 c         1197 g          728 t
ORIGIN      
        1 ccttccgggc gcaccacgcc ttccagcacc tcgacctcgc gcaggggaat gccgagcttt
       61 tcgtaggtct tcaggatttc cggatcgacc tcgtcgagcg agccgatcgt cttcttcggc
      121 ttcggcgcgg agtagtaata gagatcctgg taatcgatct tcggataatt gacgcgggcc
      181 catttcggct cggtcatggt cagccagcgg cgataggctt ccagccgcca ctccagcatc
      241 caggccggtt cgttcttttt agcagaaatg aagcggacgg tatcttccga tagccccttc
      301 ggggccttgt cggactcgat aagcgtctca aatccatatc gatattgatc gacgtcgatc
      361 tggcgaaccc gatcgacggt ctcttgtacg gcagacattc tcttcctccg ctcgcggttt
      421 caaggaccgc ggtggatcat ttcaaagctt tacgatgttt cttggcggaa atcacctgct
      481 ttagaaccgt tcaagcagtg tttcgtcgct acccttaagt agggtaccgg cgagctttcg
      541 ccaagcctta agacagcggt caatgtccgc tgacgacgtg gaccagccct gactgagccg
      601 cacggctcct tccgcgattt cggggccgaa tcccatcgct ttgagcacgt gggagggttg
      661 tacctttccc gacgaacagg cggaacccga ggaaaccgcg ataccttcga ggtcgaaacc
      721 gatgacggcg gtctccgcct tcagtccggg aaccgtaaaa agcgtagtat tcggcagccg
      781 cggcacatcg ccggagaaca caattgcccc tgttgtctcc cgcagacccc gctcgagccg
      841 attccttaag ctttcgaggc ggatggtatc gccctcccgg gtgcccatgg cggcctttgc
      901 cgccgcgcca aaccccgcga ttcccgcaac attctcggtt ccggcccgat gccccaattc
      961 ctgaccgccg ccgcgcagca acggttcaag gccccgaacg ccttcagcca gagccaaggc
     1021 gcccaccccc tttggcccgc cgatcttatg tccggaaacc gtcaccagat cggcattcaa
     1081 cgctttgata tcaaaagata tttttccgag cgcctggatc gcgtcaacgt gcaggagccc
     1141 gccggcggca tggacgatct cggcaacttg cgccaccggt tgcacggcgc cggtttcgtt
     1201 gttggccagc atcaccgaca ccagcgcggg aggcccggcc tcaagctttg tgcgcaggtg
     1261 atccagatcc agcaggccgg cgctggttac gccgatcatg ctgatcgcct ctgctggaaa
     1321 ccgcccgccg gccagcaccg aggcgtgctc gatggccgag accagtagcc gttcgaccgg
     1381 aagccctgag ccgcgccgca gcccaggcgt cagcgccagt gcattcgcct cggttccacc
     1441 cgaggtgaag acgacgttgc gaggcagagc gccgaccgca ctggcgaccg ccgcccttgc
     1501 ctcctcgacc agtctgcggg cctgacggcc ctcggcatgg accgaggacg ggttgccgca
     1561 caaatcccac gccgcggcca tcgcctcccg ggcctcaggg cgaagcgggg tggtcgcatt
     1621 ccagtcgagg tagacgcgat cgggcatatt tcgtcttagc acttttcctc gggcgacaaa
     1681 tcattgtgcc ggttgggctg tcggctgcac cggggtcggc tcgaatgcgg ccacgcgcaa
     1741 cccgaacagc ggccttaaga tggctattcg cctgatggtc tcataggtca gcacgcagac
     1801 cgccaatgtg cccgatatca cgatgctcgc ttccagccag accggaagat cgcggccgtg
     1861 cagttcgtgc gcgatcatga tgatcgcggt ctgatggacg atataatagg gaaagatcgc
     1921 atcggtaaga tatcggcgca cgcctgagtc cgccgtgagc cagcgcctgg caaaaccgag
     1981 cacggcgacg atgcagagcc actgatagca gccgtaggcg atcccgccat agagttttag
     2041 cagcagcgac ggtggcgtcg ccgactctct ggtccaccgc aacacgagaa aggacagaaa
     2101 gagcgccgcc gcgagcgaca gggccaaccg gcgctggctc tcgatcgcgt cccaaaaagc
     2161 ctccgcgcga gccagcagaa agccaagcag gaagacgctg gcgtaaaccg cgtgattgta
     2221 ccaatccccg aacagcgcat gcgtggaagg aaagttcggc agcacggcaa ggcggtaggc
     2281 cgcgaacgta accgatcgca cgatcaggag ccgcacgccc gctagcgctg gcacgagcct
     2341 ccgttccatc cagtccacaa gcccgggaac cgcgacccaa acggcgccaa gcgccatggt
     2401 gtaaatccac aagtaagcca cgaaccacag gtggttccat gtcggcagca gaatgcaggg
     2461 accgggacgg cagaactgcg gcccgaaagc aaaataatga cgcgcataga aatcagcgaa
     2521 tccagcggga tatcccagac tttcaacaat ctggtcgtag gcctgcggcg gcacgatcac
     2581 gagcatgccg aagatcagcg ggatcagcag ccggaccgat cttgaacgaa gcagcggccc
     2641 aatggggtat ttgcccaaca tgaatcgggt tgcggcgccc gagaccagaa acagcagcgc
     2701 cagccgccac ggattgagca ccagcatcag cggctccagc gccgtgatgc ggtgtgcgct
     2761 cttgatgtgg aacccccagg acacgtagag catgccgacg tggtagaaga tcagcagccc
     2821 gaaggcgccg atccggaccc aatcgaggtc gacgcggcgc tcctggcctt gagcggcgaa
     2881 atgcggtgaa tgtgatacag tttgtcgctt catggttcga tccccaaatg cgcgatgggc
     2941 ctgatgagtg acgtcaaaaa tgcgaaaggc ggccccggcg tcgagccggt ttgggaccag
     3001 aaaccacctg cctgggatga agtgagaccg cgtgggacaa gcggcggcct tggcgggata
     3061 tccggtgccg actggctgct ctatggcgcg gtcgcagcgg tcgcgctggg aatcgggcta
     3121 gtcaacgccc tctccaaagc gcaggacgtc gcctggcgcg gcggcgtcta tgatgcccgc
     3181 acgcaactgt tctgggagct gtcgagcgtc gtcacgatca tcctggtggc gcccgtgttg
     3241 ttcgtcgcgg tgcggcggat gcgccacgtc cggggatggc cgctccgcat tgccctcgcg
     3301 gtggcggcga tactcgcttt ctcgagcctg cacatcgccg gcatggttgg ccttcgcaag
     3361 cttgcgatgt tgctggtcgg cagtgcgtac gattttcact tctcggccgc gacccggtct
     3421 ggctgcgcga cggttcaacg cgcatccgca tcgaaccccg cgagattctc tggatcagct
     3481 cggccggaaa ttacgtcgaa tacagccttg ccgacggcgt caatcacctc gtccgcggca
     3541 ccctcgccgc cgaggaagcg cggctgacgc cgttcaatat cgtgcgggtg caccggacgc
     3601 ggctggtcaa cctcacgcgc gtgcgcggac taaagacagg tgcgaacggc gatttcgaac
     3661 tgacgctcga caccggccag accatctccg gcagccgccg ttaccgcggc gcagtcgcct
     3721 cgattgaaga gcttgccgcc aatcccgcct cggttctgac caataacggc gcacattcct
     3781 gatgcggcct gccgggaaac ctcggccggc cgccacaagc cgctaatttc tttcggcatt
     3841 acaaaacctt attatgggat tttaatcgct aaacgaggcc gcccgggtgc acaaaaccct
     3901 tgctttttgc ccctcgcctt atgctagaac gcggcctcca gttcaccgag cgcccgttcg
     3961 cttaagccca acggcgcctg atccgcccct ttatctctgc tgagattcat ctcgcgaaac
     4021 agc
//
LOCUS       DASWGM010000730         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_351978,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000730 DASWGM010000000
VERSION     DASWGM010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351978"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VGA15_20460"
     CDS             complement(<1..802)
                     /locus_tag="VGA15_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX9590106.1"
                     /translation="MSDVTSETQTEKTFDPEAFAMNLARAMESSGQALAAYLKPRENG
                     DPKDKPPSELTEVVKTFSTIAEYWLSDKARISELQMKMGKAYLDLWGSSMRRLVGEQA
                     PPAIEPSPRDKRFKDPEWKSNQFFDFVMQLYLLTTQWAHDLVHHAEGVDPHTRKKAEF
                     YVAQITNALAPSNFVLTNPEVLRETLASSGGNLVRGMKMLAEDIEAGQGTLRIRQSDP
                     SNLVVGVNMATTPGKVIFQNELMQLIQYAPSTENVLRTPLLIVPPWINK"
     gene            1059..1349
                     /locus_tag="VGA15_20465"
     CDS             1059..1349
                     /locus_tag="VGA15_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590107.1"
                     /translation="MPVDRKTSALAVGLLLLSAVTLGGCSSTIADMPLVGTPADAPAR
                     SKDAGAYLPVHDLPPDREEAEMTPAEQAKALKELAAARDRQATVAAKNPAAK"
     gene            complement(1408..>1639)
                     /locus_tag="VGA15_20470"
     CDS             complement(1408..>1639)
                     /locus_tag="VGA15_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590108.1"
                     /translation="IDDIGPGIARGRLGPVDLLEHVRRQTANAVKIFHRSQAPEAGQG
                     KIVRLLGVRAESDSAQKSHKINDLGQIQAPNR"
BASE COUNT          299 a          485 c          499 g          356 t
ORIGIN      
        1 atttgttgat ccacggcggc acgatcaaaa gcggcgtgcg cagcacgttc tcggtcgagg
       61 gcgcatactg gatcagctgc atcagctcat tctggaagat caccttgccc ggcgtggtcg
      121 ccatgttgac gccgacgaca agattggaag gatcggactg acggatccgc agcgtgcctt
      181 gtccggcctc gatgtcttcc gccagcatct tcatgccgcg aacgaggttg ccgccgctgg
      241 aggccagcgt ttcgcgcaat acttccggat tggtgagaac gaaattcgac ggcgccagcg
      301 cgttggtgat ctgcgcaacg tagaactcgg ccttcttgcg ggtatgcgga tcgacgcctt
      361 cggcgtggtg gacgaggtcg tgcgcccatt gcgtcgtcag cagatagagt tgcatcacga
      421 aatcgaaaaa ctggttcgat ttccactcgg ggtccttgaa tcgcttgtcg cgcggagacg
      481 gttcgatcgc cggcggcgcc tgctcgccga caaggcggcg catcgatgag ccccacagat
      541 cgagataggc cttgcccatc ttcatctgca gttccgaaat ccgcgccttg tccgacagcc
      601 aatattccgc aatggtgctg aaggtcttga ctacctcggt caattcgctg ggcggcttgt
      661 ccttggggtc gccgttctcg cgcggcttga gataggccgc gagcgcctgg ccgctgctct
      721 ccatggcccg cgcgagattc atcgcgaagg cttcaggatc gaacgtcttc tcggtttggg
      781 tttcgctggt aacgtcgctc ataaccggac actacaggtt cgtttagggt tcgtcaccgc
      841 gttgatcgac catgaacggt tccggattaa ccctgacatc acatcctgtt gcactgcgac
      901 aaagtatcct gcttctgtca cattgaagcc ccagcggttc gatttcttgt tcaggacttc
      961 atcctttcgg gtgacaatgg ttgagctcgg cgcccgcaac tgatgatata attgtcaggc
     1021 ggctgcaaag cttgggtgaa gtggggactt ccagaccggt gccggtggat cgaaaaacaa
     1081 gcgcacttgc ggtgggactg ctgttgctgt cggcggtcac gcttggcggc tgctcgagta
     1141 cgatcgcgga tatgccgctg gtcggcacgc cggccgacgc acccgcccgt tccaaggacg
     1201 ctggcgccta ccttccggtt catgatttgc cgccagaccg cgaggaggcc gagatgaccc
     1261 ctgcggagca ggccaaagcg ttgaaggaat tggccgcggc ccgcgaccgc caggcgacgg
     1321 tggccgccaa gaaccccgcc gcgaagtgag gcctggcgta ctattacggc tggcgcggtc
     1381 gggcgcgcgt ggtaaaacaa ccccgattca acgatttggc gcctgaattt gccctaaatc
     1441 attgattttg tgtgatttct gcgcggaatc cgattccgct ctgacgccaa gcagcctcac
     1501 gatcttgccc tgaccggctt ccggagcttg agaccgatgg aagattttta ccgcattcgc
     1561 cgtctgccgc cttacgtgtt cgagcaggtc aaccgggcca aggcggccgc gcgcaatgcc
     1621 ggggccgata tcatcgatc
//
LOCUS       DASWGM010000731         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1175518,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000731 DASWGM010000000
VERSION     DASWGM010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175518"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..952
                     /locus_tag="VGA15_20475"
     CDS             185..952
                     /locus_tag="VGA15_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590109.1"
                     /translation="MTFTLETRDLTIRFGGHVAVNRVSCTFRPGELTAIVGPNGAGKT
                     TYFNLISGQLRPSDGSILLDGADITQLSAPLRTRAGLGRAFQLTNLFPNLSVEENVRL
                     AVQSESGVHYEMLRPWTTRRDLIARADAILDKVALGGRRSVPATALSHGDQRKLEVAL
                     MMALEPKVFMFDEPTAGMSIDEVPVVLNLIAQLKQDASKIILLVEHKMDVVRSLADRI
                     IVLHNGQLVADGKPADVIASPIVQEAYLGIAPGKSAA"
     gene            949..1671
                     /locus_tag="VGA15_20480"
     CDS             949..1671
                     /locus_tag="VGA15_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590110.1"
                     /translation="MTDLLTLAGVHTHIGRYHILHGIDFAVPEGQSTMLLGRNGAGKT
                     TTLRTIMGLWQASAGRISLDGQSIEHSATPDIARLGVGYVPESMAVFSDLTVKENLVL
                     AARDGPLDDSRLEWIFGFFPALRRFWLSRAGSLSGGQKQMLSIARAIVEPRKLLLIDE
                     PTKGLAPAIVVALIECFAEVKRRGATILLVEQNFHAARELGDKVLVMDNGMIVHRGEM
                     AALASDVALQERLLGLSLETHQ"
     gene            1668..>2365
                     /locus_tag="VGA15_20485"
     CDS             1668..>2365
                     /locus_tag="VGA15_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590111.1"
                     /translation="MTDLAAPAALLKPRRDILPVLLPVILALAMLPLIGSASSWVTLT
                     VASLAMGMMIFIMASGLTLVFGLMDVLNFGHGAFISVGAYAATLVLLPLAPYVQADSL
                     WLNLAVLAPAAVLSMAVSGALGLVVERVLILPVYGQHLKQILMTTGGLIVAEQALYAL
                     WGPQIIPLPLPTSLRGSFIIGDIAIAKYRLLAMLVGLIVFAGIQLVLNRTKIGLLIRA
                     GVENREMVEALGYR"
BASE COUNT          401 a          756 c          749 g          459 t
ORIGIN      
        1 ggagtcgtcc gacgcctccg acgtttaaac cgtcattgcc gggcttgacc cggcaatcca
       61 tctcgcttcc gaataaaatc gctttggaag cggatggatg cgcggatcaa gtccgcgcat
      121 gacgctcgga atatgcggca acgctggcga tcgacgagat gacagacctg ccgatggcgc
      181 tcgaatgacc ttcacgctcg aaacccgcga tctgaccatt cgcttcggcg gacacgtcgc
      241 ggttaaccgc gtcagctgca cctttcgccc cggggaactg acggccattg tcggtcccaa
      301 cggggccggc aagaccacct atttcaacct gatctccgga cagctgcgcc ccagcgatgg
      361 cagcatcctg ctcgatggcg ccgatatcac ccagctttcc gcgccgctgc gcacccgcgc
      421 aggccttggc cgcgcgttcc agctcaccaa tttgtttccc aatttgagcg tcgaagagaa
      481 tgttcgcctc gccgtgcaat ccgaaagcgg cgtgcattac gagatgctgc ggccgtggac
      541 gacgcgccgc gacctgatcg cccgagccga cgctattctc gacaaggtag cacttggcgg
      601 ccggcgaagc gtgccggcga ccgcgctgtc gcacggcgat cagcgcaagc tcgaagtcgc
      661 cctgatgatg gcgctcgagc cgaaggtctt catgttcgac gagccgaccg ccggcatgag
      721 catcgatgaa gtccccgtcg tgctcaatct gatcgcgcag ctcaagcagg atgccagcaa
      781 gatcatcctc ttggtcgagc acaagatgga cgtggtgcgt tcgctggccg accgcatcat
      841 cgtgctgcac aacggacagc tggttgccga cggcaagccg gccgatgtga tcgcttcccc
      901 gattgtgcag gaggcctatc tcggcatcgc gcccggaaag agcgcggcat gaccgacctt
      961 ctcaccctcg ccggcgtgca cacccatatc gggcggtatc acatcctgca cggcatcgat
     1021 ttcgcggtgc ccgaaggcca gtccaccatg ctgcttggcc gcaacggcgc cggcaagacc
     1081 acgacgctgc gaaccatcat gggcctgtgg caggcatccg cggggcggat atcgctcgac
     1141 ggacaatcca tcgagcacag cgcgaccccg gatatcgcgc ggctcggcgt cggttatgtt
     1201 ccggagagca tggcggtgtt ctccgatctg accgtgaagg aaaacctcgt tctggcggcg
     1261 cgggacgggc cgctcgacga cagccgtctg gagtggattt tcggattctt tcccgcgctc
     1321 aggcgcttct ggctgtcgcg cgccggcagc ctgtccggcg gacagaagca gatgctttcc
     1381 attgcccgcg ccatcgtcga accgcgcaag ctgttgctga tcgatgagcc caccaagggt
     1441 ctggcgcccg cgatcgtggt ggcgctgatc gagtgctttg cggaagtcaa acgccgcggc
     1501 gccaccatct tgctggtcga acagaatttc cacgcggcgc gcgaactcgg cgacaaggtt
     1561 ctggtgatgg ataacggcat gatcgttcac cgcggcgaga tggcggcgct ggcgagcgac
     1621 gtagcactgc aggagcggct gctgggctta agtctggaga cgcatcagtg accgaccttg
     1681 ccgcacccgc cgcgttgctc aagccgaggc gggatattct gcccgtgctg ctgccggtga
     1741 ttcttgcatt ggcgatgctg ccgttgatcg gatcggcaag ctcctgggtg actctgacgg
     1801 tggcgagcct cgccatgggc atgatgatct tcatcatggc ttccggcctc acgctggtgt
     1861 tcggcctgat ggacgtgctc aatttcggtc acggcgcctt catctcggtc ggcgcctatg
     1921 ccgcgacgct ggtgctgttg ccgctggcgc cttacgtgca ggccgactcg ctttggctca
     1981 atcttgcggt gctggcgccg gccgccgtgt tgtcgatggc ggtgtccggc gcgcttggcc
     2041 tcgtggtcga acgggtgctg atcctgccgg tctacggcca gcacctcaag cagatcctga
     2101 tgacgacggg cggattgatc gtcgccgaac aggcgctgta tgcgttatgg ggcccgcaga
     2161 tcattccgct gccgctgccg acgtcgttgc gcggctcgtt catcatcggc gacatcgcaa
     2221 tcgcgaagta ccgcctgctg gcgatgctgg tcggcctcat cgtgttcgca ggcattcagc
     2281 tggtgctgaa ccggaccaag atcggcctcc tgatccgcgc cggcgtggaa aaccgcgaga
     2341 tggtggaagc gctcggttac cggat
//
LOCUS       DASWGM010000732         4993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1175812,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000732 DASWGM010000000
VERSION     DASWGM010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175812"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            743..1954
                     /gene="thrS"
                     /locus_tag="VGA15_20490"
     CDS             743..1954
                     /gene="thrS"
                     /locus_tag="VGA15_20490"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX9590112.1"
                     /translation="MDDLDHRNLGVKLKLWHLQEDAPGMVFWHPRGYAVYRVLEDYVR
                     RKMRRAGYAEVRTPQLLPKELWGRSGHWDKFGEHMFRVDDGELAMALKPMSCPCHVQI
                     FNKGLRSWRELPIRYAEFGACHRNEPSGSLHGIMRTRAFEQDDAHVFCREQDVEGEVA
                     CFIALLGEAYRDLGFPDYEVTLATRPAARAGDDATWDWSEAKLGDAARRCGLAPQINP
                     GEGAFYGPKLEFALRDRLGRSWQCGTVQLDGVLPQRLEASYVAPDGSRARPLMIHHAI
                     FGSLGRMIAILLEHHGGVLPFWLSPDQVAVAPISKDQAGYGADVLAAFEDAGIRAVAY
                     DGADTLSRRIVAAHEMAVPVMAIVGGREMRDGRVSLRERDGSQADVPLAEAVSRLQAR
                     ARPGALVAEAL"
     gene            2829..3071
                     /locus_tag="VGA15_20495"
     CDS             2829..3071
                     /locus_tag="VGA15_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590113.1"
                     /translation="MARARKSRTQNTVAADGLPGDLYDYARPPARRVGRPAPEDKTTW
                     TVTDDWPEDVPVTEAEIEVFEAWFGDLFDELFSTRH"
     gene            3088..3582
                     /locus_tag="VGA15_20500"
     CDS             3088..3582
                     /locus_tag="VGA15_20500"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9590114.1"
                     /translation="MTVPIRAALYLRVSTNRQADNDLSIPDQRRQGKGYCKAHGWEIV
                     ADYVEPGASATDDRRPEFQRMIDAATTKPPMFDVIVVHSFSRFFRDQFQLEFYVRRLA
                     KNGVRLISITEELGDDPMSNMIRQIMALFDEYQSKENAKHTLRAMKENARQGFWNGAL
                     PPIG"
     gene            3589..4362
                     /locus_tag="VGA15_20505"
     CDS             3589..4362
                     /locus_tag="VGA15_20505"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9590115.1"
                     /translation="MAGEQRGHRIKKTLEIDPFQAETVRLIFRLAWEGDGTSGAMGVK
                     SITKHLNAAGIRTRDGGRWGIDAVHKVLTRTTYIGRHRFNTKFWKTRERKPEAEIIEM
                     AVPPIIEVAEFEAVQALLKSRSPALTAPRIVSGPTLLTGICFCAACGMAMTLRTGKSG
                     RYRYYTCSTKARQGETGCRGRTVPMEKLDKLVADHIEHRLLQPSRLEKNTVLGSRPPR
                     GARGAPNCAYRRIAQAGGRGRRQTQAALRRHREWCHRSL"
     gene            4497..4703
                     /locus_tag="VGA15_20510"
     CDS             4497..4703
                     /locus_tag="VGA15_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590116.1"
                     /translation="MRTESGGYRRDYLRALAQRVEVDAEELRIMGSKNELLRTLVAAS
                     SAKTAGLGVPSSVPKWRTRHDSNV"
BASE COUNT          975 a         1573 c         1584 g          861 t
ORIGIN      
        1 cgtagatctg ctgccgtaaa atcatcccga agctcaaccg ctgcgctcat ttgttgctcc
       61 ccgaatcaaa tcgtgaagcc caaatgaatc acaacccgcc gaaaactccg agagtcagtc
      121 cctacgcaat ctggtataac ctatcacatg ccgtaacccg agaatatgcg accgcctgcg
      181 atttggtact gcgctcaaag ggggcgcttt ccgatgtcgc gggcgtctcg gtgtgcgtcc
      241 atggacaggg caccgaacca tcctgggtac cggtgccatg tccgttcccg gggacgaact
      301 ggaaatcgaa ggcaggacgc caaaccgacg cgaatgaccc tgaagagaca tcgggatgga
      361 ggcaaggagc cgctggccat cgatcatcaa cgaaaagacg tttgccgact tcaagcgcgt
      421 tgctgagcag accggcattc tgacaaggag ccggatcatc cgccacagga gtgttgatgt
      481 ccccgcgcat catccatatt ccgcgacgcc aagcggctgg cgttgacgcc tgagcgtttt
      541 accgccatat acagagcatg aataagcttc tatcagccgc attgaccacc acggcgaccg
      601 cttacggcgg atcgcctgct ggttgcgcgc gctagagaca agcgtacttc acccagaaaa
      661 ggccccgccg gcgacggacg gggccttttc tggtttttga tggtccccgt ccgcgctttt
      721 tcccatcgca aggaacacag ccatggacga tctcgaccac agaaatctcg gcgtgaagct
      781 caaactctgg caccttcagg aagatgcgcc gggcatggtg ttctggcatc cgcgcggcta
      841 cgcggtctac cgggtgctgg aggactatgt ccggcgcaag atgcgccgag ccggctatgc
      901 cgaggtccgg acgccacagc tgctgccaaa ggagctctgg ggccgcagcg gccactggga
      961 caagttcggc gagcacatgt tccgggtcga cgacggcgag ctggcgatgg cgctgaaacc
     1021 gatgtcgtgc ccgtgccacg tgcagatctt caacaagggg ctgcgctcgt ggcgggagct
     1081 tccgatccgc tatgccgagt ttggcgcttg ccaccgcaat gagccttccg gttcgctcca
     1141 cggcatcatg cggacgcgcg ccttcgagca agacgacgcg catgtgttct gccgcgagca
     1201 ggacgtggag ggcgaagtcg cgtgcttcat cgccctgctg ggcgaggcct accgcgacct
     1261 cggttttccc gactacgagg tgacgctggc gacgcggccg gcggcgcgcg ccggcgacga
     1321 cgcgacctgg gattggtcgg aggcgaagct cggcgatgcc gcgcggcgct gcggcctcgc
     1381 gcctcaaatc aaccccggcg aaggtgcgtt ctacggaccg aagctggaat tcgcgctgcg
     1441 cgaccggctg ggacgatcct ggcagtgcgg caccgtgcag ctcgacggcg tgctgccgca
     1501 gcggctcgag gcgtcctacg tcgcccccga cggcagccgc gcgaggcccc tgatgatcca
     1561 ccacgccatc ttcggatcgc tcggccgcat gatcgcgatc ctgctcgagc accatggcgg
     1621 cgtgcttccg ttctggctgt caccggacca ggtcgcggtc gcgccgatct cgaaagacca
     1681 ggccggatac ggcgcggacg ttctggcggc gttcgaggat gccgggatcc gggccgttgc
     1741 ctatgacggc gccgacaccc tttcgcggcg catcgtggcg gcgcatgaaa tggcggtgcc
     1801 ggtgatggct atcgtcggcg ggcgcgagat gagggacggc cgggtgagcc tgcgcgaacg
     1861 cgacggctca caggccgacg ttccgctggc cgaggcggtg tcccgcctgc aggcgagggc
     1921 ccggccgggc gcccttgtgg ctgaagcctt gtagcccgcg gacgcaggat cctaggcgag
     1981 caggccctag gaagaggggg tatgtcgcat gctgtgcaca aaaccgacat gtagtgatgt
     2041 ccgacgtgag tccgttattg aatgaagatt ggacgtgaaa ccggcatccc gaactcggta
     2101 aatcatgcgg ccggcggcgc ttacaatgcc gtccaattgt catccgcgtc ggtggccgag
     2161 tgcactgttt tcaacgggcg cacgacggct caaaggccgt tgaccgacgc agcgccgagc
     2221 aatcggcgtg cagaggaatc tgcaggcccg ctggcgcttg atgcgcaatt tcagaggagg
     2281 gaaatatcgt agcgtggagt agacgttagg cgctcgatga ggacggcgag ataaaagcga
     2341 cgcctccggt cgcgctgatt gtggttgtgg gagttgaagt taggtagacg tcgcaaggat
     2401 cgacctgcca tcgctttttg cgaaacgcca acgacatcaa tcttccgagc gacggcaggc
     2461 atttttatgc gcgcttacgg ctcagtgtga agcgatccgc taactgctct tgacgacgct
     2521 tttgcttgag cgcgacgcgt cgaatcgtga ctgtgcacgc ggctcccatt ggcctaagaa
     2581 tttattagcc tgctgatgac ctgctgatga caagtgagcc ggaccagcga caccccgtca
     2641 aggctcgcgc tccgcgcgac cggctccgca ggctggcggc cttgaccggc tgccgcgtgg
     2701 gctggtcaag caggtaaggc aagttgggag cgaggtcgct cccgtcgcca caacgatgga
     2761 ggccgcggcg tagcgtcgct tgcgcttcgg ccattctttt ggcgcagggg tggtgcgaca
     2821 ctgcggtcat ggctcgcgct cgcaaatccc gaactcagaa taccgtcgct gcggatggac
     2881 tgcccggcga tctctacgac tatgcgcgcc caccggctcg ccgcgttggc cggccggcac
     2941 cggaggacaa gacgacctgg accgtcacgg acgattggcc cgaggacgtg ccggtgaccg
     3001 aggccgagat cgaggtgttc gaagcttggt tcggggacct gttcgacgag ttgttttcga
     3061 cccgccactg acttggaagg aaccgtcatg accgtgccca ttcgtgccgc cctctatttg
     3121 cgggtctcca ccaatcggca ggcagacaac gacctctcga tcccggatca gcgccgccag
     3181 gggaagggct actgcaaggc acatggctgg gagatcgttg ccgactatgt cgagcccggc
     3241 gcgtcggcca ctgacgaccg gcggccagaa ttccagcgca tgatcgacgc cgccacgacc
     3301 aagccgccga tgttcgacgt gatcgtggtc cacagcttca gccgcttctt ccgtgaccag
     3361 ttccagcttg agttctacgt ccgccggctc gccaagaacg gcgtccgact gatctcgatc
     3421 accgaggaac tcggcgacga tcccatgagc aacatgatcc gccagatcat ggcgctgttt
     3481 gacgaatacc agtccaagga gaacgccaag cacaccttgc gggccatgaa ggagaacgcc
     3541 cggcagggtt tttggaatgg cgccctgccg ccgatcggct agcgcatcgt ggccggcgag
     3601 cagcgcggcc accgcatcaa gaagaccctg gagatcgatc ccttccaggc cgagaccgta
     3661 aggttgatct tccggctggc atgggagggc gacggcacct cgggagcgat gggcgtcaag
     3721 tccattacca agcatctcaa cgccgccggc atccggaccc gcgacggcgg acgctggggc
     3781 atcgatgccg tccacaaggt gctgacccgg acgacctata tcggccgtca ccgcttcaac
     3841 accaaattct ggaagacccg cgagcgcaag ccggaagccg aaatcatcga aatggcggtg
     3901 cctccgatca tcgaggtcgc cgagttcgag gccgtgcagg cgttgctgaa atcccgcagc
     3961 ccggcgttga cagcgccgcg catcgtcagc ggcccaactc tccttaccgg catttgcttc
     4021 tgcgcggcct gcggcatggc gatgaccctg cgcaccggca agagcggccg gtataggtat
     4081 tacacctgct cgaccaaggc gcgccagggc gagaccggct gcaggggccg tactgtcccc
     4141 atggaaaagc tcgacaagct cgttgccgat cacatcgaac accgcctgtt gcagccgagc
     4201 cgtctcgaga aaaatactgt cctcggttct cgaccgccgc gaggagcgcg cggagcgcca
     4261 aactgtgcat atcgccgaat tgcgcaagcg ggcggccgag gccgacgcca aactcaggcg
     4321 gctctacgac gccatcgaga atggtgtcac cgatctctct gacctgatgc tgaaggaccg
     4381 tatcactgag ctgaaggcca tccgggacca ggcccgcgcc gggcgcgatc aatcgacagg
     4441 cgccaaccat caccacccaa tcgatcaagt cgtttgccag gacggcccgc aagcggatgc
     4501 ggaccgagag cggcggctat cgccgcgatt accttcgcgc gctcgcccag cgcgtcgaag
     4561 tcgacgcgga agaacttcgc atcatggggt cgaaaaacga actgctgcgc acgctcgtcg
     4621 ccgcttcaag cgcaaaaacg gcgggtcttg gcgtgcccag ttctgtaccg aaatggcgca
     4681 cccgacacga ttcgaacgtg tgacctttgc cttcggaggg cacggctcga ataacggccc
     4741 atgtttaacc gaaagccctt cgaattgcca aaggcgagtt atgggttgaa agggagagcg
     4801 cggcgctacc gctgcgtcct ctcgcgccgg acatgtggcg gccggccgaa agcccgatgc
     4861 gcgcaaacag attgcggcgg actccgttga cgccatcgag cggggacacg acatatcctc
     4921 taccgtcgat ccaggtcact gctcaggagc aagatatgaa cgcgccgcca aacatcgatc
     4981 ccgtcaccac gca
//
LOCUS       DASWGM010000733         5498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2310696,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000733 DASWGM010000000
VERSION     DASWGM010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310696"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VGA15_20515"
     CDS             complement(<1..641)
                     /locus_tag="VGA15_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEX9590117.1"
                     /translation="MTQKLQRAPARASERGFVIVAVLWILAALAALATIFSVYLSNSA
                     QALAVHDTGLKAEALVSASLELTAYQLLLAGDKARPALGSFHFRLDNADVLVSFTSEA
                     ARIDLNFAPKEMLANLFAGLGADQQAAKEYADRIVGWRTRPTANAANDEEALYGAAGL
                     AYSPRQALFAHVNELSLVLGLTPVLIDRALPFVTVFSGSSNVDVLVAPPEIIAA"
     gene            complement(638..1423)
                     /locus_tag="VGA15_20520"
     CDS             complement(638..1423)
                     /locus_tag="VGA15_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9590118.1"
                     /translation="MTASPARQRGGEDGFSLIETLVAMALMGLVLSALANITAQWLPN
                     WNRGLERIQRSELIGIALQRIGADLAAAEYVSANRDARHPLFEGSELSVTFVRTALGP
                     NAGPGLDVVRVGETTDRQGLVTVRSRAPFQPLPLASSLSEQIHFGEPVLLLRAPFRLS
                     FAYAGQDRIWKSNWHDADKLPAAIKLTVRDAASERILSISTVAPVHVQSPAQGECAQP
                     DGGCDGKSGAPNNPQGSPQGNSQGNLQGNSQAGNAATGQGGSR"
     gene            complement(1420..1833)
                     /locus_tag="VGA15_20525"
     CDS             complement(1420..1833)
                     /locus_tag="VGA15_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX9590119.1"
                     /translation="MSPAAPPPIDGRDAGFTILEVLIALAVVAVSIVAIGSVMSTNVR
                     GVRLLEQHVTLMQTARTVMTAGIPPRAELGPGVLSGQIDDHKWTIDVGPMGEGWDVPG
                     ADVAWIPALVRIRVRSSSGAVSDLRTVRLMHRPPQ"
     gene            complement(1805..2269)
                     /locus_tag="VGA15_20530"
     CDS             complement(1805..2269)
                     /locus_tag="VGA15_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX9590120.1"
                     /translation="MSDGAERGFTLLEMVCVLALIAMMAAVLLPFIPHNTSRSRLQAY
                     ALQAATLLKADRNAAIRRGADVATLVDAGARSIRSGASADMIRIPDDVRFEALLPQTC
                     NRRAALSTISFFASGMSCGGTIALTRLDAGYEIRVNWLTGRIEIVSRSSATN"
     gene            complement(2262..2759)
                     /gene="gspG"
                     /locus_tag="VGA15_20535"
     CDS             complement(2262..2759)
                     /gene="gspG"
                     /locus_tag="VGA15_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX9590121.1"
                     /translation="MRSAKRVGGFSAGRAEDGRQQDFGQQGFTLVEMLVVITIIGLIM
                     GLIGPRVLNYLSESKVKAAKIQLQSFGSALDLFYLDAGRFPSTAEGLAALVRRTPGVA
                     AWNGPYLKGGNVPNDPWNNAYLYRSPGDRGPYDIMSYGSDGQEGGSGLAADISIENMA
                     SAKNE"
     gene            complement(2778..3992)
                     /locus_tag="VGA15_20540"
     CDS             complement(2778..3992)
                     /locus_tag="VGA15_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX9590122.1"
                     /translation="MPNFRYRALTQNGEIVHGTLSAPTAAEVAHRIEYLRLLPIETIE
                     DKATVAASSSGFGFFGRPSLAEVTTFTRDMALLLKAGARLDDALELLADDADVGRLRP
                     VVGKLRAALLTGESFAGAVADHPALFPAMYVALVRVGEVSGTLDQVLEMLGAERARAE
                     LMRRKLTDAMQYPTFVLIAAACVMLFFILFVLPQFSAVLQDFGAKSDSALSVFIKLSD
                     FLRANAAVMMFASASFVAGVWYLLRRPGAGAAIMTAASGVPGIAGVFQFYRTSLFCRN
                     LGVLLGSGVNLTATLRILVDIMAVTGSVTTWTTAADRVRHGGKLSDALLASSNMPPMA
                     IRMLRLGEETGQLPVLAGRVAEFYEAKLQRSLDRIVGIVGPLAIVTISTVVGGLIVSV
                     MTALLSVTQLVG"
     gene            complement(4002..>5498)
                     /locus_tag="VGA15_20545"
     CDS             complement(4002..>5498)
                     /locus_tag="VGA15_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX9590123.1"
                     /translation="DRVTLQDMLSAPSLVAAFSQRFLREMMLFPYQSADGGAVLAVAD
                     PTDTAARRAAEIVLRADVAIKVASVEDLAIVLDQRLGGDDVDLLGGSEPYQPREDDIE
                     SLRDLASGAPVVRAVNDLLEKAVELRASDIHIEPFHTGLVVRMRIDGLLRPVAAPAGV
                     LPQAVISRIKIVANLNIAERRLPQDGAARLRAGRTDIDIRVAIMPMQHGESAVIRILP
                     KDRGLLVVEKLGFSPADDATLRRLLKLPHGMIVITGPTGSGKTTTLATILSILNEPGR
                     KILTVEDPVEYDIPGVNQAQIKPAIGLTFAAALRSFVRQDPDVIMVGEVRDSETAHVA
                     VHAALTGHLVLTTLHTESAAAAVPRLLDLGVEGYLLRSTLRAVVAQRLVRQLCDRCKV
                     PKTLVEADFVEDPRLGALGFRSGETIQGPCGCERCGGTGYRGRLGVFEILELTNEVRE
                     LIAEKTDGLKIDQTAIRSGMTTMLDDGVAKCRAGLTSPAEILRVTIAR"
BASE COUNT         1092 a         1765 c         1698 g          943 t
ORIGIN      
        1 gcggcgatga tctccggcgg cgcgaccagc acatcgacgt tggacgatcc gctaaagacc
       61 gtcacgaacg gcaaagcgcg atcgatcagg accggagtca ggccgagaac gagactgagc
      121 tcattcacat gggcaaagag cgcttgccgt ggagaataag cgaggcccgc ggcaccataa
      181 agcgcctctt cgtcgttggc ggcattggcc gtgggacgcg tccgccaccc gacgatccgg
      241 tcggcatatt ccttggcggc ctgctgatcg gcaccgagac ctgcaaatag attggccagc
      301 atctccttgg gcgcgaagtt gagatcgatg cgggcggctt ccgaggtaaa gctgacaagg
      361 acgtccgcat tgtccaaccg gaagtgaaac gaccccagcg cgggacgcgc cttgtcgccc
      421 gccagcagta attgataggc cgtgagttcg aggctggccg acaccagggc ctccgccttc
      481 agtccggtat catggacggc caacgcctgc gccgaattgg acagatagac cgaaaagatc
      541 gtcgctagcg ccgccaaggc cgcgagaatc cacagcacag cgacgatcac gaagccgcgc
      601 tccgaggccc tcgcaggcgc gcgctgaagt ttttgcgtca ccgcgatcct ccctgccctg
      661 tcgcagcgtt tccggcctgt gaatttcctt gcaaatttcc ttgcgaattt ccttgagggc
      721 ttccttgggg attattcgga gcaccggatt tgccgtcaca accgccgtcc ggctgggcgc
      781 actcgccttg cgctggcgac tggacatgaa ccggcgccac cgtcgaaatc gagaggattc
      841 gttcgctcgc cgcatcgcgc accgtcagct tgatggccgc cggcagctta tcggcgtcgt
      901 gccagttgct cttccagatc cggtcctgac ctgcataggc gaacgacagc cgaaatggag
      961 cccgcaacag caacaccggt tcgccgaaat gaatttgctc tgacagggac gatgccagcg
     1021 gcaacggctg gaatggcgcg cgcgagcgga ccgtcacgag gccctgacga tcggtagttt
     1081 cgccgacccg aaccacatcc agcccgggcc cggcgttcgg gcccaatgcg gtccgcacga
     1141 atgtcaccga cagctccgag ccctcgaaca acggatggcg cgcgtcgcga ttggccgaca
     1201 catattcggc ggccgccagg tcggcgccga tccgctgcag cgcgatgccg atcaattcac
     1261 tgcgctggat gcgctcaagg ccgcgattcc agtttggcag ccattgcgcg gtgatgttgg
     1321 ccagcgctga aagcaccagg cccatcaacg ccatcgccac gagagtctcg atcagcgaaa
     1381 agccgtcctc ccctccccgt tgccttgcag gcgaagcggt cattgcggcg gcctgtgcat
     1441 caggcgaacg gtgcgaaggt cggacacagc gccggaggag gatcgaaccc gaattctgac
     1501 gagtgcggga atccaggcga cgtccgcgcc aggcacatcc cagccctcgc ccatcggtcc
     1561 gacgtcgatc gtccacttat ggtcgtcgat ctgtcccgat aatacaccgg ggccaagttc
     1621 ggcgcgcggc gggatgcccg cggtcatgac cgtgcgtgcg gtttgcatca atgtcacgtg
     1681 ctgctcaagc aagcgcacgc cgcgtacgtt tgtcgacatg acagatccaa tcgccacgat
     1741 ggagacggca acgaccgcca gcgcgatcaa cacttcgagg atcgtgaagc cggcgtcgcg
     1801 accgtcaatt ggtggcggag ctgcgggaga caatttcgat ccttccggtc agccagttga
     1861 cacgaatttc gtagccggca tcgagccggg tcagcgcgat ggtcccgccg cacgacattc
     1921 cgctggcaaa gaagctgata gtcgacaagg ccgcacgccg gttgcaggtt tgcggcaata
     1981 gcgcctcgaa gcggacgtcg tctggaatac ggatcatatc cgcgctcgcg cccgaccgga
     2041 tcgagcgcgc tccggcatcg accagcgtgg cgacgtccgc cccgcgccga atcgccgcgt
     2101 tgcggtccgc cttcagaagc gtcgcggcct gaagcgcata ggcctgcagg cgggagcggg
     2161 acgtattgtg cggtatgaac ggcagcagca cagccgccat catggcgatg agcgcaagca
     2221 cacacaccat ctcaagcaac gtgaagccac gttccgcgcc gtcactcatt tttcgcgctg
     2281 gccatgtttt cgatggaaat gtcagccgcc agaccgctgc cgccttcctg gccatccgat
     2341 ccataggaca tgatatcgta agggccgcgg tctccgggcg agcgatatag ataggcgttg
     2401 ttccagggat cgttggggac gttgccacct ttaagatacg gtccgttcca ggcggctaca
     2461 cccggtgtcc ggcgcaccag cgccgccagg ccctccgcgg tcgaggggaa ccggcctgca
     2521 tcgagataga acagatcgag cgcgctgcca aaactctgta gctgaatctt ggcggccttg
     2581 actttcgatt cgctgagata attgagaacg cgcgggccga tcaggcccat gatcaggccg
     2641 atgatggtga tcaccaccag catctcgacc agcgtgaaac cctgctggcc aaagtcctgc
     2701 tgacgcccgt cttccgcgcg cccggcggaa aatccgccaa cgcgttttgc cgatctcatg
     2761 atgaaccaac ccttgctcta tccgacaagt tgcgtaaccg acagaagcgc ggtcatgacc
     2821 gatacgatca atcccccgac cactgtgctg atggtgacga tggcaagcgg gccgacgatg
     2881 ccgacgatgc ggtcaaggct gcgttgcaat ttcgcctcgt agaattcggc gacccggccc
     2941 gccagcaccg gcaattgccc ggtctcttcg cccagccgca gcatgcggat tgccatcggt
     3001 ggcatgttgc ttgaggcgag cagagcgtcc gaaagcttgc cgccgtgacg gacgcgatcg
     3061 gctgccgtcg tccaggtcgt aacgcttccg gtgactgcca tgatgtcgac caggatgcgc
     3121 aaagtagccg tgaggttgac gccgctaccg agcagcacgc cgagattccg gcaaaacaga
     3181 ctggtgcggt aaaattgaaa gacgccggcg ataccgggaa cgccagaggc cgcggtcatg
     3241 atcgcggcgc ctgcgcccgg ccggcgaagc aggtaccaga caccggccac gaacgaagcg
     3301 gaggcaaaca tcatgacggc cgcgttcgcc cgaagaaagt ccgacagctt gatgaaaacg
     3361 ctgagcgcgg aatccgattt agcaccgaag tcctgcagca ccgcagaaaa ctgcggcagc
     3421 acgaacaaga tgaagaacag catgacgcag gccgcggcaa tcagaacgaa ggtcgggtac
     3481 tgcattgcat cggtgagttt gcggcgcatc agttcggccc gcgcccgctc cgctcccagc
     3541 atctcgagca cttgatcgag cgtgcccgat acttcgccga cgcgaacaag cgcgacgtac
     3601 atggcgggaa acaaagccgg gtgatccgca accgcgcctg cgaaactttc tccggtcaga
     3661 agtgccgccc gcaatttgcc gaccaccgga cgaagccggc cgacatcggc gtcatcagcg
     3721 agcaattcga gggcgtcgtc caggcgcgcg cccgccttca aaagcagggc catgtcccgg
     3781 gtgaatgtcg ttacttccgc caggctcggc cggccgaaga aaccgaaacc ggagcttgaa
     3841 gcggcaacgg ttgccttgtc ttctatggtt tcgatcggca gcagtcgcag atactcgatg
     3901 cggtgagcaa cttccgccgc cgtcggagcc gataacgtgc cgtgcacgat ctctccgttc
     3961 tgcgtcaggg cccggtagcg gaaattcggc acgatctcac ctcaccgcgc aatcgtaacg
     4021 cggagtattt ccgcgggcga ggtcaggccg gcgcggcatt tcgcaacgcc gtcgtcgagc
     4081 atcgtcgtca tcccggagcg gatcgcggtc tgatcgattt tcagtccatc ggtcttttcc
     4141 gcgatcagtt cgcgaacctc gttggtgagt tcaagaatct cgaacacgcc aagccggccg
     4201 cgatagcccg taccgccgca gcgctcgcac ccgcaggggc cctgaatggt ttcgccactc
     4261 cggaagccga gcgcgcccag tcgggggtct tcgacaaaat ccgcctcgac gagcgtcttt
     4321 ggcactttgc agcggtcgca cagctggcga accagccgct gggcaaccac ggctcgcaag
     4381 gtggaccgca gcagatatcc ctcgacgccg agatcgagaa gccgcggcac cgcggcggcc
     4441 gccgattcgg tgtggagtgt cgtcagcacg aggtgaccgg tgagggccgc atgaacagcg
     4501 acgtgagcgg tttcggagtc gcgcacctcg ccgaccatga tcacgtccgg atcctgacgc
     4561 acgaatgaac gcaacgccgc tgcgaacgtc agtccgatcg ccggcttgat ctgtgcctgg
     4621 ttgacgccgg gtatatcgta ttcgacggga tcctcgacgg tgaggatttt tcgacccggc
     4681 tcgttcagga tcgagaggat cgtggccaat gtcgtggttt ttccgctgcc ggtcgggccg
     4741 gtgatgacga tcatgccatg gggcagcttc agcagtcgtc tgagcgtggc atcgtcggcc
     4801 ggcgaaaatc ccagcttttc gaccaccagc aggccgcgat ccttcggcag gatgcgtatg
     4861 accgcggatt ccccatgctg catcggcatg atcgcgacgc gaatgtcgat atcggtacgc
     4921 ccggcccgca gccgcgccgc gccatcctgc ggaagccggc gctccgcgat gttgaggttg
     4981 gcgacgatct tgatgcgcga aatcacggct tgcggcaaga cgccggccgg cgccgccacc
     5041 ggccgcagca ggccgtcaat ccgcatgcgg accaccagcc cggtatgaaa gggctcaatg
     5101 tgaatgtcgc tggcgcgcag ttctactgct ttctccaaga gatcattcac ggcgcgaaca
     5161 accggcgccc cgctggcaag atcgcggagg ctttcgatat cgtcttcgcg tggctgatac
     5221 ggttcgctgc cgcccagcaa atcgacatca tcgccgccga gacgctgatc gaggacgatc
     5281 gcgagatctt cgaccgacgc caccttgatc gcgacatcgg cccggagcac gatttcggct
     5341 gcccgccggg ccgccgtatc ggtcggatcg gcgaccgcca gcacggcgcc tccgtcggcg
     5401 gattgatatg gaaacagcat catttcccgc agaaaccgct gcgaaaatgc cgcgaccaga
     5461 gatggcgcgg acagcatgtc ctggagcgtg acccgatc
//
LOCUS       DASWGM010000734         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2594293,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000734 DASWGM010000000
VERSION     DASWGM010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2594293"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VGA15_20550"
     CDS             <1..783
                     /locus_tag="VGA15_20550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590124.1"
                     /translation="MHVKDKVCVVTGAASGIGAAVARAYAQAGARGVVVADLKSSRDR
                     LAGVAGDIDGFAVTADVGQESDIQALIAAAEAKYGPVDIFFSNAGLSRKGQETAVDAD
                     WDVSWRVHVMSHVFAARALVPGMLARGSGYLLNTASAAGLLASLNSMPYGVTKHAAVA
                     LAEHLAIQYGDRGIRVSVLCPQSVQTGMTTPGPSAARVDGVMVPAEVARIVVEAMEAE
                     RFLILSHPQVHEYMQRKASGIDRWLAGMRRLRDKTYGAQRSD"
     gene            complement(1030..1719)
                     /locus_tag="VGA15_20555"
     CDS             complement(1030..1719)
                     /locus_tag="VGA15_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX9590125.1"
                     /translation="MKTVSAQKLDIEIGNAGSVSALLIRPTPAHTCFVFAHGAGAGMT
                     HPFMEAVAAGLGERGVATLRYQFPYMEQASKRPDPPGIAQATVRAAVAEAERHCADLR
                     LIAGGKSFGGRMTSQAQATAPLAGVHGLAFLGFPLHPAGKPSSDRARHLADVHVPMLF
                     LQGTRDNLAELSLLEPVVDRLGPSASLHRVEGADHSFHVPARSGRKDSEVMREILDTF
                     AAWIEVKRAVS"
     gene            1944..2993
                     /gene="bioB"
                     /locus_tag="VGA15_20560"
     CDS             1944..2993
                     /gene="bioB"
                     /locus_tag="VGA15_20560"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX9590126.1"
                     /translation="MSTSLDSIDAGNTSDQPRHDWTRAEAEALYDLPFADLIFRAQGI
                     HRRNFDPNHVETASLLSIKTGGCPEDCGYCSQSAHYETGVKATRLMDQSAVVATARRA
                     RDAGASRFCMAAAWRNPKDKDLDRVCEMVAAVKELGMETCATLGMLTGDQARRLRQAG
                     LDFYNHNVDTSPEFYGQIITTRTLEDRIDTLGHAREAGLKICCGGIVGMGEQVGDRLG
                     MLMLLANLSVHPESVPINLWNEVKGVPVNDTAERPDPIALVRMIAVARIMMPRSVVRL
                     SAGRQYMTDELQALCFVAGANSIFIGDVLLTTKNPQTDRDTNLLGRLGMTSKLDDAVP
                     AAVQIAIQAGVQAAI"
BASE COUNT          558 a          947 c          992 g          548 t
ORIGIN      
        1 atgcacgtca aagacaaggt ctgtgtggtt acgggagcgg cgagcggcat tggtgcggcg
       61 gtcgcacgtg cctatgcgca agccggcgcg cgcggcgtgg tggtcgccga tctcaagagc
      121 tcccgcgacc ggcttgccgg cgtcgcgggt gacatcgacg gctttgctgt gaccgccgat
      181 gtcggacagg aaagcgatat ccaggcgctg atcgccgcgg cggaagccaa gtatgggccg
      241 gtcgacattt tcttctccaa cgccgggctc tcgcgcaagg gacaggagac cgccgttgat
      301 gccgactggg acgtgagctg gcgggtgcac gtcatgagcc acgtgttcgc ggcgcgggcg
      361 ctggtgcccg gcatgctggc gcgcggctcc ggctatttgc tgaacaccgc atccgcggcg
      421 ggcctgttgg cgtccctgaa ctcgatgccg tatggggtca caaaacatgc cgcggtggcg
      481 ctcgcagagc atctcgccat tcaatatggc gaccgcggca tccgcgtttc cgtgctgtgt
      541 ccgcagtcgg tgcagaccgg catgaccact cccggcccga gcgcggcaag ggtcgatggc
      601 gtgatggttc ctgccgaggt ggcgcgcatc gtggtcgagg cgatggaggc ggaacgcttt
      661 ctgatcctgt cccacccgca agtgcatgag tacatgcagc gcaaggcctc cggcatcgac
      721 cgctggctgg ccgggatgcg gcggctgcgc gacaagacct atggcgcgca gcgctcagac
      781 tgacgtccgc gcttcgaact cgtgttcttt ttgcggttaa cggtttggca aaattgtatc
      841 ggcaaaggcg gcgcgctccc tctccccttg tgggagaggg ttggggtgag gggtcacggt
      901 ctatcgttgt gccgccaccc ctcacccgga tcgcatctat cgatgcgatc cgacctctcc
      961 cacaagggga gaggtggcgc cgagcatgcg gaagcggaat cccagactct aaccggatca
     1021 atcgcgcatt caggataccg cacgcttcac ctcgatccac gccgcaaacg tatccaggat
     1081 ttcgcgcatc acctcgctgt ccttccgtcc cgagcgcgcc ggcacgtgga aggaatgatc
     1141 ggcgccctcg acacgatgca acgacgccga tggcccgagt cggtcgacaa cgggttcgag
     1201 cagcgacaac tccgcgaggt tgtcgcgggt gccttgcaaa aacagcatcg gaacgtgcac
     1261 atcggcgagg tgtcgggcgc ggtcgctgga tggcttgccg gccggatgca gcggaaagcc
     1321 caggaaggca aggccgtgga caccagccag cggcgccgtc gcctgcgcct gcgatgtcat
     1381 ccgtccgccg aacgattttc cgcccgcaat cagccgcaga tccgcacaat gtcgctcggc
     1441 ttccgccaca gccgcccgca ccgtggcttg cgcgatgccc ggcggatcgg gccgcttgct
     1501 ggcttgctcc atatagggaa actgatagcg cagtgtcgcc acgccacgct cgccaaggcc
     1561 ggcggcaacc gcttccatga agggatgggt cattccggcg ccggcgccat gggcaaatac
     1621 aaaacaggtg tgcgccggag tgggacggat cagaagcgcg gagacggagc ctgcattccc
     1681 gatctcgatg tcaagctttt gcgcgctgac ggttttcatc tcatctaccc ggtattcact
     1741 acgaggttat agcacgctcc acccgataag ggcgtttcgg cgcgcgccaa tctccgtcga
     1801 tggcgtcgat atcggcgcga aggcagggat gcgcaaaccc ggattgacag gattttgagc
     1861 gtgtggtcct aagtgccgcg gctgcggaga aaatcgtttc cgtgaattcg accccgcggg
     1921 ggtgaaagcc gaaggatcgt ttgatgagta cgtcgctgga ttcgatcgac gccggcaaca
     1981 catcggatca gccgcgtcat gactggaccc gagcggaagc cgaagcgctc tacgatctgc
     2041 cgttcgccga cttgatcttc cgggcgcaag gcattcatcg ccgcaacttc gatcccaatc
     2101 acgtcgagac cgcgagcctg ctcagcatca agaccggcgg ctgcccggaa gattgcggct
     2161 actgctcgca gagtgcccac tacgagaccg gcgtcaaggc gacccggctg atggatcaat
     2221 cggcggttgt cgcgaccgcg cggcgcgcca gggacgccgg cgccagccgg ttctgcatgg
     2281 ccgccgcctg gcgcaatccc aaggacaagg atctcgatcg ggtgtgcgaa atggtcgccg
     2341 cggtcaagga actcggcatg gagacctgcg ccacgctggg catgctgacc ggggatcaag
     2401 cgaggcggct gcggcaggcg gggctggatt tctacaatca caatgtcgac acctcgccgg
     2461 aattctacgg ccagatcatc acgacccgca ccttggagga tcgcatcgat acgctcggcc
     2521 atgcccgcga agccggactc aagatctgct gcggcggcat cgtgggaatg ggagaacagg
     2581 tcggtgaccg cctgggcatg ctgatgctgc ttgccaacct ttcggttcac cccgaaagcg
     2641 ttccgatcaa cctttggaat gaggtcaagg gcgttcccgt caacgacacc gccgagcgac
     2701 ccgatccgat cgcgctggtg cggatgatcg cggtggcgcg aatcatgatg ccgagaagtg
     2761 tggttcggct gtccgcgggc cgccaatata tgacagacga attacaggcg ctgtgttttg
     2821 tggctggcgc caattccatt ttcatcggcg atgtgctgct gaccaccaag aatccgcaga
     2881 ccgatcgcga taccaatctg ctcggccgcc tcggcatgac gtccaagctg gatgacgccg
     2941 tgcccgccgc ggtccaaatc gcgatccagg ccggggttca agccgcgatt tgacgttgat
     3001 ccgcttcgaa gccgggatcg tcctgaagcg aaccttggct ttgcc
//
LOCUS       DASWGM010000735         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2032674,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000735 DASWGM010000000
VERSION     DASWGM010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2032674"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VGA15_20565"
     CDS             complement(<1..485)
                     /locus_tag="VGA15_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX9590127.1"
                     /translation="MRTDTTSAASAIFAANRAQGSVTFDVHQVEGVTRRRQLHESGSL
                     RVRFPSPEAEGLSAVFVNTAGGVAGGDRFDIDISIGEGSHLAVTTAAAEKIYRAQGPA
                     AQLNIALKLAAGSHLAWLPQETILFDRARLKRRIDIDLAEGASLLLCEIVVFGRAAMG
                     ER"
     gene            complement(671..1366)
                     /gene="urtE"
                     /locus_tag="VGA15_20570"
     CDS             complement(671..1366)
                     /gene="urtE"
                     /locus_tag="VGA15_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEX9590128.1"
                     /translation="MLDVKDINLYYGAAQALRGVSISAQPGKVMCVLGRNGVGKTSLL
                     RAMVGQYPIASGSITFNDADITGLKPYERARRGIGFVPQGREIFPLLTVEENLKTGFG
                     PLKRDDRNIPDDVFSLFPVLQTMLGRRGGDLSGGQQQQLAIGRALVMRPKLLLLDEPT
                     EGIQPSIIKDIGRAISYLRSLGNIAIVLVEQYLDFACELGDNFAVMDRGAVKFACDRS
                     TMDPAEISRQMAL"
     gene            complement(1458..>1929)
                     /locus_tag="VGA15_20575"
     CDS             complement(1458..>1929)
                     /locus_tag="VGA15_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9590129.1"
                     /translation="TVFESQTIEDNLLLALNVDHRVRGTLFWRETRDESERIERVLET
                     IRLTDARDRLAGSLSHGQKQWLEIGMLLAQDPKLLLVDEPVAGMTDVETHQTAELLKE
                     INKNKTIMVVEHDMTFVRELGVKVTCLHEGTVLAEGTIDQVSSNERVIEVYLGR"
BASE COUNT          356 a          618 c          574 g          381 t
ORIGIN      
        1 cgctccccca tcgcggcgcg gccgaatacc acgatctcgc agagcagcag cgaggcgccc
       61 tccgcaaggt cgatatcgat ccggcgcttt agccgcgccc ggtcgaacag aatggtctcc
      121 tgcggcagcc aggcgagatg cgaacctgcg gcaagcttca gggcgatgtt gagctgcgcc
      181 gcgggccctt gcgcccggta gattttttcc gccgccgccg tggtcaccgc gagatgcgat
      241 ccctcgccga tcgagatgtc gatgtcgaag cggtcgccgc cggcgacgcc gcccgcggta
      301 ttgacgaaaa ccgccgagag accttccgcc tccggtgagg gaaatcgaac gcgcaacgag
      361 ccggattcat gcagctggcg gcggcgggtg acgccctcga cctgatgcac gtcgaacgtc
      421 acggagccct gggcacggtt ggccgcgaaa atcgccgaag cagcactcgt ggtatcagtc
      481 cgcatccgcc ccaaaccttc ccaaaaaggg cgcgcgaccg ctgccggtcg cgttacacat
      541 tgcaagatca tgatccgatt agatcgaatc ggatcatgat ctcatccttt tgtttgagca
      601 tgatcttttc ggaaaaccgg ttcccaccca tcgggtcgtg gcccgagggc atgcttttcc
      661 ggatcatgct ctacagcgcc atctggcggc tgatttcagc gggatccatc gtacttcgat
      721 cgcaggcaaa tttcacggcg cctcggtcca tgaccgcgaa attgtcgccg agctcgcagg
      781 caaagtcgag atattgttcg accagcacga tcgcgatgtt gccgaggctg cgaagatagg
      841 agatcgcccg cccgatgtcc ttgatgatcg acggctgaat gccctcggtc ggctcatcga
      901 gcagcagcag tttcggccgc atcaccagcg cgcggccgat cgcgagctgc tgttgctggc
      961 cgccggacag atcgccgccg cggcggccga gcatcgtctg cagcaccggg aacagcgaga
     1021 acacgtcatc gggaatattg cggtcgtcgc gcttgagcgg cccaaaaccg gtcttgagat
     1081 tttcctccac cgtcagcagc ggaaagatct cgcggccctg cggcacgaag ccgatgccgc
     1141 gccgcgcccg ctcatagggt ttcaagcccg tgatatcggc gtcgttgaag gtgatcgagc
     1201 ctgaggcgat cgggtattgg ccgaccatgg cccgcagcag gctggtcttg ccgacgccgt
     1261 tgcgccccaa cacgcacatc accttgcccg gttgggccga aattgagacg ccgcgcagcg
     1321 cttgcgccgc gccgtaatag aggttgatgt ccttgacgtc gagcatgtgg catgtcctca
     1381 agagggcgag aagtgagtag cgaatagtaa gagagagctc gctttccatt cgctattcgc
     1441 tactcaccat tcgcccctca cctccccaaa tacacttcga tcacccgctc attcgacgaa
     1501 acctgatcga tggttccctc cgccagtacc gtgccttcgt gcaggcaggt gaccttgacg
     1561 ccgagttcgc ggacgaaggt catgtcatgt tcgacgacca tgatcgtctt gttcttgttg
     1621 atttccttga gcagctcggc ggtctgatgg gtttcgacgt cggtcatccc ggcaacgggt
     1681 tcatcgacca atagcaattt cggatcctgc gccagcagca tgccgatctc cagccattgc
     1741 ttctggccgt gcgacaggct ccctgccagg cggtcgcgcg cgtcggtcag gcgaatggtt
     1801 tccagcaccc gctcgatgcg ctcggattcg tccctggttt cccgccagaa cagcgtgccc
     1861 cgcacgcggt gatcgacgtt gagcgccagc agcagattgt cttcgatggt ctggctttcg
     1921 aataccgtc
//
LOCUS       DASWGM010000736        10864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2594725,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000736 DASWGM010000000
VERSION     DASWGM010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2594725"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VGA15_20580"
     CDS             <1..494
                     /locus_tag="VGA15_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX9590130.1"
                     /translation="IARRALDDEPLLSGLGPEPLGNEFDAAMLARSCANKKTSLKAAL
                     LDQRVVAGLGNIYVCEALFRSHLSPRRLAATLATKKAEPTDHAKRLVDAIHAVLNQAI
                     KAGGSSLRDHRQTSGELGYFQHSFQVYDREGEKCQTDGCSGIVKRFTQNGRSTFWCPK
                     CQK"
     gene            complement(755..1516)
                     /locus_tag="VGA15_20585"
     CDS             complement(755..1516)
                     /locus_tag="VGA15_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX9590131.1"
                     /translation="MLTLVLATQKGGSGKSTLAIGLALAAVQAGHTVRLIETDSQGTL
                     SNWQARRVDAEPIVEPVYKAREIEQRLQSLERSGVTLTIIDTPGGINAATTEAIRYSD
                     FCLIPARPSIADIEATAPTLHVIRAWKKPFAFVLNQTPIRGHRISNAATAIGDEAAFD
                     VADVVAQPFIVMRNDHQDALSAGLAVSELSPKGKSAEEIRGLWHWIGTRLNIGQQNAS
                     PTFDEQEIVIEFPVAPEIDPAMFLRAPARTAALGS"
     gene            1773..3452
                     /gene="ggt"
                     /locus_tag="VGA15_20590"
     CDS             1773..3452
                     /gene="ggt"
                     /locus_tag="VGA15_20590"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX9590132.1"
                     /translation="MTSNWRDRTDTVFNCRKQPASGSRGMVVSNHPLASAAGAEMLAA
                     GGNAIDAAIATLFTLTVVEPMMVGIIGGGMAHIRLSDGSHRFIDGQSTVPLAVSPDTY
                     RSKPGSAHDVFDTVDNENLNGPKAVAVPGSLKAWCETLRRFGTMSLADVMQPAIKHAS
                     RGFAATPYLHECIVDSAKEMRQDKAISAIYLPNGTPLNAGERVVQAEYAKTLATISRD
                     GEAALYQGPLGDILVDYMKAHGGFVSREDLASYRTVERSPVRADYRGWEILGPPPPAA
                     SGVHIAQMLNILEGYDIAAMAFGSAATIHHLAEVLKIAFADRAAASGDPDFVGVPVER
                     LTSKAYADERRRAIDAGRAQQWGPGVAQIEGAHTTHMTAADAMGNVVATTQTINNLFG
                     AKFLIPGLGTVPNNYMNLYDPRPGHALSLAPGKRITTSMSPVMALRDGKLVYALGLPG
                     GKKIFPSALQALLNLIDHGMSLQEAVEAPRIWTEGNALEVELAVPDAVRAKLTAMGHK
                     VVAVPTVAGGMNAIQFHDDGSLTGAACWRADGTPIGLAGGLARAGVRFGLA"
     gene            3729..5723
                     /locus_tag="VGA15_20595"
     CDS             3729..5723
                     /locus_tag="VGA15_20595"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9590133.1"
                     /translation="MSKATDRTVSLRVPTAMTASDLVVGVYASLASLRGWLITGNDAF
                     KAERAGLWKDIQTHGAEMDNLSSRWTVEKNKQDWMQAKPLLDELRNAQDKAEAIAHTI
                     DEQPAAKLLATEAAPLAKLMLQKATSIIDEEGNIASTDQRKSLLIGFADMRGSMAMAI
                     GAIRAYLLTAEPAFKTEFVELWALNQKKFDALSKRRSEMTSDQQKAFDSLVEARAKFS
                     PLPQKMFDIRASDRWNMAQWFLTNEAAPRANKLLDIFAGAKNAEGSRSGGMVTRQQDN
                     LKTDGAAVLAETSFLTTLLWVLMGIGIGVAVTVVYLTNRSIVPPILKMVGAMGRLAGG
                     DHSVEIPATDKKDEIGLMARAVLIFKENMIKAKELAAKEAEAISERVARAARVNDLTA
                     TFDKDISAVLRSVASASTELQATASSMTATAEETSNQATAVAAATEEASTNVQTVAAA
                     SEELASSVTEISRQVAQSAAITQKAVAEADRTNSTVQGLFNDAASIGDVVKLISDIAG
                     QTNLLALNATIEAARAGEAGRGFAVVAAEVKSLAEQTAKATDQISAQVSSIQNSSSEA
                     VNAIKGITATINEINEIASAIASAVEEQGSATQEIARNVQQAALGTGEISSNVAGVQQ
                     AAGDTGAAAHQVLQASSELSRQSETMRGQVESFLSNIKAA"
     gene            complement(5811..6977)
                     /locus_tag="VGA15_20600"
     CDS             complement(5811..6977)
                     /locus_tag="VGA15_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9590134.1"
                     /translation="MKHIQQTIFTGLSILLAVANPRHARADEVGVSSNTILFGQVAAL
                     EGRSSALGQGMRQGILAAFAEINAKGGVHGRKLELISRDDGYDPDRSVAQTIKLIEED
                     QVFALIGAVGTPTTTATVPIATAKHVPFIGPFTGAAFLRAPDLHNVVNIRASYAAEAE
                     AWIKHLTEHLHVKSIAIFYQDDSFGRDGLAGVMLALEKRSMELTAEGTFERNTKAVGS
                     ALRTLKRAEPEAVVMVGTYGPCAEFIKLAHKSGFNPIFVNISFVGANALAKELGPEGQ
                     GVIISQVVPFPWDKSVKVVADYQAAEKALDPNLQPEFVSLEGYLSGRLVASALEETGP
                     NPTRADMLRVINDVGRFDISGTIMTFGPKMNDAPAKVFLTVIQPDGTFKAVKEL"
     gene            7315..9738
                     /locus_tag="VGA15_20605"
     CDS             7315..9738
                     /locus_tag="VGA15_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9590135.1"
                     /translation="MIERRRIPASRSRLYRPALVAAGIGLLLSLAGASAVGQWEERVT
                     KVEFEGVAETQAIIMQNGMNEYVSRLAALRTLFESANEEITRSEFETFSGRLFERHPG
                     ILRIGWIPRINRKERAEYEAAAVGDGISGYRIKSLLADGGIATAPQSDEYFPIFFSTE
                     PKTSVAYGLDYSTDPARRATLERARDNDLVATLRAPLYEPKNGIRPLGVLVCVPVYVK
                     GTSRATIADRRRNLAGFVVGVFNLRLLLESIRTTTAATPAVSINVYPSDMGRSAGPEN
                     RFVPDFSSEPGASQSAFADGPHWSGTLKIADASWHVRVTPTADGRLTTRHERALTVLA
                     AGIVITTFLAVYLGLASRNSRQLALANRRVLELAQTDILTGLPNRAFFLEQLEDADSY
                     ERRGSGAFSILMLDLDRFKNVNDSLGHAAGDALLRQVALRLKSAIRSTDVLARLGGDE
                     FAIIQAECDDQRTGSIDLATRISKLIAEPFLLQGNQVEVGTSIGISMAPEHGSDQEQL
                     LKKADLALYRSKSAGRNCFTLYDEAMSAELEARNTLESDLRDAVARCQFELHYQPFFD
                     VQTGQRRGLEALVRWRHPTRGLIPPDQFIPLAEETGLIVPLGEWVLRRACDDATSWPA
                     DIKVAVNLSPVQLKQVELFDVIQSALRSSGLPPERLEIELTESVLLERAVENHALMER
                     LKSIGISLALDDFGTGYSSLSCLTAFPFDKIKIDRSFIGNLTKRHKSSAIISSIVTLA
                     RGLNLSVTAEGVETSEQFERLKTLGVNFAQGYLLGRPMPLGDPKVSLNQIMRTWVQLC
                     VSSRAVASG"
     gene            complement(9763..>10864)
                     /locus_tag="VGA15_20610"
     CDS             complement(9763..>10864)
                     /locus_tag="VGA15_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX9590136.1"
                     /translation="EFQKERGGKPTGVLNPQERGALAETARRRQDNVGWKIVTDAGSG
                     VRLGIPTKLVPQQSSDANGARWSSPTATIQIQLARRKEANPTTAKLAEKEKKEPAGRA
                     VDYTVVKPDFFVLSGLQGLKKFYVRGTFKGDEVRILTILYDQATENTVEPIVIAMSSA
                     FNPFPSGAQIAGPPPRKTVEYGTGIVVSDDGAIVTDRQITDGCLSIAIAGFGNADRVA
                     EDKDHDLALLRIYGARGLKPLGLGNGAAKTSVELTGVADPQNQGGGAAASSVKATTTQ
                     VGSGNDLALAPAPAVGFSGAAAFDADGKFAGIALLKSVVVAGPPNAAPAAQATLVSAD
                     TVREFLKANAVNAADGSSEVKASVLRVICVRK"
BASE COUNT         2081 a         3289 c         3428 g         2066 t
ORIGIN      
        1 tcattgcccg ccgggcgctc gatgatgagc ctctgctaag cggtctcggc cccgaaccgc
       61 tcggcaacga attcgacgcc gccatgctgg cgcgctcctg tgccaacaag aagaccagcc
      121 tgaaggcggc gctgctcgat cagcgcgtgg tcgcaggcct cggcaacatc tatgtctgtg
      181 aggcgctgtt tcgttcgcat ctgtcgccgc gaagattggc cgcgaccctg gcgacaaaaa
      241 aagctgagcc gaccgatcac gccaaacgcc tggtcgatgc aattcacgcg gtgctgaatc
      301 aggcgatcaa ggccggcggc tcatcgctgc gcgatcatcg ccagacctcc ggcgaactcg
      361 gctatttcca gcactcgttc caggtttacg accgcgaagg cgaaaaatgc caaaccgacg
      421 gttgcagcgg catcgtcaag cgttttaccc agaacggccg ctcgacgttc tggtgtccga
      481 aatgccagaa atgagtcttc gtgttcgcct ctggtgtggc cgtcatcctg aggcgcgaag
      541 ccgctgtcgc ggcgagcctc gaaggatgcg agttctgcac ctgtggccca tccttcgagg
      601 ccgtcgcttc gcgccggcac ctcaggatga cgtggcaagt tcggcgacgc caagtgtgga
      661 tcactagcca aagccaggcg ccgcgctccc tcccgctaat tggagaggga gtgcgctgtc
      721 gttatcgttc gcgtcaactt gaacggataa agtttcagga acccagtgcc gccgtcctcg
      781 cgggtgcacg caagaacatc gcgggatcga tttcgggcgc taccgggaat tcgatgacga
      841 tctcctgttc gtcgaaggtc ggcgaagcgt tttgttggcc gatattcagc ctggtcccga
      901 tccaatgcca gaggccgcga atctcctcgg ccgatttacc cttcggactg agttcgctca
      961 cggccagtcc ggcgctcagc gcatcctgat ggtcgttgcg catcacgatg aacggctgcg
     1021 cgacaacatc ggcaacgtcg aacgccgctt cgtcgccaat cgcggtcgcc gcgttgctga
     1081 ttcggtggcc gcgaatcggc gtctggttga ggacaaaagc gaatggcttc ttccaggcgc
     1141 ggatgacatg aagcgtcggt gcggtggctt cgatatcggc gatgctcggg cgcgccggga
     1201 tcaggcagaa gtcggaatag cgaatcgcct cggtcgtggc ggcgttgatg ccgcccgggg
     1261 tgtcgatgat cgtcagcgtt acgccactgc gttccagaga ctgcagccgc tgctcgatct
     1321 cgcgagcctt gtaaaccggc tcgacgatgg gttcggcatc gacgcggcgc gcctgccagt
     1381 tcgaaagcgt gccttgagaa tcggtctcga tcaggcgaac ggtgtgcccg gcttgaacag
     1441 ccgcaagggc gaggccgatg gcaagcgtac tcttgccgct gccacccttt tgggtggcca
     1501 atacgagcgt aagcattgaa gattcctcgg aatgttttta cggagatcta cggttacgct
     1561 gaactgagtt gcatcgtgtt acgatgccga gcgattctcg ctcaggacgt gccggcccga
     1621 tccaaggctc acaggatcgc ggctatccga atcattacgc gcaacgatgg ctcactttgg
     1681 ggaatatggc tattccgggg taatactgac gcgagccgac catgcaataa agtgtcagca
     1741 gtggcagtcc tgcaacagca cagggtgaaa aaatgaccag caactggcgg gatcgcacag
     1801 acacggtttt caattgccgg aagcagccgg cgtcgggcag ccgcgggatg gtggtcagca
     1861 accacccgct ggcgtcggcc gcgggtgcgg agatgcttgc cgccggcggc aacgccattg
     1921 atgccgcgat cgcaaccctg ttcacgctga ccgtcgtcga gccgatgatg gtcgggatca
     1981 tcggcggcgg catggcgcat atccggctca gtgacggcag ccaccgcttc atcgatggcc
     2041 aaagcacggt tccgcttgcc gtcagcccgg acacctatcg atcgaagccg ggttcggcgc
     2101 acgatgtatt cgataccgtc gacaatgaaa atctaaacgg tccgaaggcg gtagccgtgc
     2161 cgggctcact caaggcctgg tgcgaaacct tgcgccggtt cggcaccatg agcctcgccg
     2221 atgtcatgca gccggcgatc aaacatgcgt cgcggggttt tgcggcgacg ccctatctgc
     2281 acgaatgcat cgtcgatagc gcaaaggaaa tgcgccagga caaggcgatc tcggcgatct
     2341 acctgccgaa cgggacaccg ctgaacgccg gcgagcgcgt cgtacaggcc gaatatgcca
     2401 agacgctcgc caccatctcg cgcgacggcg aagcggcgct gtatcagggg ccgctcggcg
     2461 acattctggt ggattacatg aaggcccatg gcgggtttgt cagccgcgaa gacctcgcgt
     2521 cctacagaac cgtcgaacgc tcgccggtgc gagccgatta tcgcggctgg gaaattctcg
     2581 gtccgccgcc gccggcggcc tccggtgtgc acatcgcgca gatgctgaac atcctggagg
     2641 gctacgatat cgccgccatg gcattcggca gcgccgcgac catccatcat ctcgccgaag
     2701 tgttgaagat cgcctttgcc gatcgcgccg ccgcgagtgg cgatcctgat tttgtcggtg
     2761 tgccggtaga gcggctgacc tcgaaggcct atgccgatga acgccgccgc gcgatcgatg
     2821 ccggacgcgc gcaacaatgg ggtccgggtg tggcgcagat cgaaggcgca cacaccaccc
     2881 acatgaccgc cgcggatgcg atgggcaacg tggtcgcgac cacgcagacc atcaacaacc
     2941 tgttcggcgc gaagtttctg attccgggcc tcggcaccgt gccgaacaac tacatgaatc
     3001 tctacgatcc ccggcccgga cacgctttgt cgctggcgcc gggcaagcgc atcaccacct
     3061 cgatgtcgcc tgtcatggcg ctgcgcgatg gcaagctggt ttatgcgctc gggctgccgg
     3121 gtggaaagaa gatttttcca agcgcgttgc aggcgctgct taatctgatc gatcacggca
     3181 tgagcttgca ggaggcggtc gaggcgccgc ggatctggac cgagggcaat gcgctcgagg
     3241 tcgaattggc ggttccggat gcggtgcgcg ccaagctgac tgcgatgggt cacaaggtcg
     3301 ttgcggtgcc gaccgtggcc ggaggcatga acgccatcca gttccacgac gacggctcgc
     3361 tcacgggcgc ggcctgctgg cgcgccgacg gcacgccgat cggccttgcc ggagggctgg
     3421 cgcgcgcggg ggttcgcttt ggactggcat gagaggctgt gcgaaccaga cgagcgctct
     3481 atcagtgcgt gaggcgccgg cgccctgtgg tgagagatcc cgtattaata ccgatcaggc
     3541 attgttccaa tcttcatggt taacggacag cgtacccttt gagcttgcct ggcggctgct
     3601 tgcatcggag ggacttcacc gtggcatttc tgcaattgaa catccgaggg cggttgattc
     3661 tcggctttag cgtgctttgc attctcctgg ccggtgttgt cggcacaaca atcatcaaag
     3721 tgcactcagt gagcaaggcg accgatcgca ccgtcagctt gcgggttccg accgccatga
     3781 cggccagcga tctggtggtg ggtgtgtatg cttcgctcgc atcgctgcgg ggctggctga
     3841 tcaccgggaa cgacgcattc aaggcggagc gcgccggcct ttggaaagac atccagacgc
     3901 acggcgccga gatggataat ctctcgagcc gctggaccgt tgagaaaaat aaacaggact
     3961 ggatgcaggc caagccgttg ctcgacgaat tacgcaacgc gcaagacaag gccgaggcga
     4021 ttgcgcatac catcgacgag cagcccgcgg caaagctgct tgcgaccgag gcagccccgc
     4081 tggccaaact gatgctgcag aaagcgacct ccatcatcga cgaggaaggc aacatagcct
     4141 cgaccgatca gcgcaagagc ttgttgattg gctttgccga tatgcgaggc agcatggcaa
     4201 tggcgatcgg cgccatccgc gcctatctgc tgaccgcgga gccggctttc aaaacggagt
     4261 tcgtggagct ctgggcgctc aatcagaaaa agttcgacgc actgtcgaaa cgccgatcgg
     4321 agatgacgag cgatcagcag aaggccttcg actcactcgt tgaagcccgt gccaaattct
     4381 cgccgctgcc gcagaaaatg ttcgatatcc gcgcctcgga ccgctggaac atggcacaat
     4441 ggttcctgac caatgaagcc gcgccccgcg ccaacaagct cctcgacatc ttcgcgggag
     4501 cgaagaatgc cgagggctcg cgttcgggcg gtatggttac gcggcaacaa gacaacctga
     4561 aaacggacgg ggcggcggtt ctggccgaaa ccagtttcct caccacgctg ctttgggtgc
     4621 tgatgggtat cggcattggt gtcgcggtga cggtcgtgta cctgaccaac cgttcgatcg
     4681 tgccgcccat tctcaagatg gttggagcca tggggcggct tgccggtggc gatcactccg
     4741 tcgaaattcc cgcgaccgat aaaaaggatg aaatcggtct gatggccagg gcggtcctga
     4801 tcttcaaaga gaacatgatc aaggccaagg aacttgccgc caaggaagcc gaagccatca
     4861 gcgagcgcgt cgcccgtgcg gcgcgggtga acgacctgac cgcaacgttc gataaggata
     4921 tatcggcggt gctgaggtcg gtggcatcag cctcaaccga actacaggcg acggcatcgt
     4981 cgatgacggc gaccgccgaa gaaaccagca atcaggcaac ggcggtggcc gcggcgacgg
     5041 aagaggcttc aaccaacgta caaaccgttg cggcagcgtc ggaggaatta gccagttccg
     5101 tcaccgaaat cagccgtcag gtagcgcaat cggccgcgat cacgcagaaa gccgtggcgg
     5161 aggccgatcg caccaattcg acggtccagg gcctgttcaa tgacgccgcg agcattggcg
     5221 atgtcgtcaa gctcatcagc gacatcgccg gccagaccaa ccttctcgcg ctcaacgcca
     5281 cgatcgaagc ggcgcgcgcc ggcgaggctg gcaggggatt cgccgtggtg gcggccgagg
     5341 tcaagagcct tgccgaacag accgcgaagg caaccgatca gatcagtgct caagtttcgt
     5401 cgatccagaa ttcatccagc gaagcggtga acgccatcaa ggggatcact gcgacgatca
     5461 acgaaataaa tgagatcgcc tccgcgattg ccagcgctgt ggaagaacag ggctcggcga
     5521 cgcaggagat tgcgcgcaac gtgcaacagg cggctctcgg caccggtgaa atctcgtcca
     5581 acgttgccgg cgttcagcaa gccgccggcg ataccggcgc cgcggcccat caggtcctcc
     5641 aggcatccag cgagttgtcc aggcaatccg agacgatgcg aggtcaggtc gaatccttcc
     5701 tcagcaacat caaggccgct tgatcacgca ccgatcagcc gtctccgcca ttcagtcatg
     5761 tgcggagacg caaggccggc ttagggcatc gcccaacggg tcacttgcgc tcacagctcc
     5821 ttaaccgcct tgaacgtgcc gtccggctga atgaccgtta gaaaaacctt agccggggcg
     5881 tcgttcatct tcggcccgaa ggtcatgatc gttccactga tgtcgaatcg tccgacgtcg
     5941 ttgataaccc tgagcatgtc cgcgcgcgtc gggttcggtc cagtctcctc cagcgccgag
     6001 gcgacaaggc gtcccgaaag ataaccctcc agagagacaa attccggctg cagattcgga
     6061 tcgagggctt tttcggcggc ctggtaatcg gcgacgacct tcaccgattt gtcccagggg
     6121 aagggcacga cttgggagat gatgactccc tggccctcgg ggccgagctc tttcgccagc
     6181 gcgttggcgc cgacaaagga gatattgacg aagatcggat taaagccgct cttgtgcgcg
     6241 agcttgatga attcggcgca tggtccgtag gtgccgacca tgaccaccgc ttccggttcg
     6301 gcgcgcttga gcgtacgcag tgctgaacca accgctttgg tattgcgctc gaaggtgcct
     6361 tcggccgtca actccatgct gcgtttttcc agcgcgagca tgacgccggc cagaccgtct
     6421 cggccaaacg aatcgtcctg gtagaagatt gcgatgctct tgacatgaag atgctcggtc
     6481 aggtgcttga tccacgcctc agcctcggcg gcatagctgg cgcgaatgtt gacgacgttg
     6541 tggaggtccg gcgcgcggag gaatgcagcc ccggtgaacg gaccgatgaa cggcacatgc
     6601 ttggctgtcg caattggaac ggtcgcggtg gtggtgggtg tgccgacggc gccgatcagc
     6661 gcgaaaacct ggtcctcctc gatcagcttg atggtctggg cgaccgagcg atcgggatcg
     6721 taaccatcgt cccggctgat cagctcgagc ttgcgtccgt gcacaccacc cttggcgttg
     6781 atctcggcga aggccgcgag aatgccctgc cgcatgcctt ggccgagcgc ggatgagcga
     6841 ccttcgagtg ctgcgacctg accgaacaga atggtgttgg aactgacgcc aacctcgtcg
     6901 gccctcgcat ggcgcgggtt cgccacggcg agcaagatcg aaaggccggt gaagatcgtc
     6961 tgttgtatgt gcttcatggt atcattctgc gggataagga tgcgcatcca ccttcgccga
     7021 acggtatccc gacgttatga agagctggct aatctcctgg cgtttgtccg atgccgcatg
     7081 atcccgcgcg cggctcacga ggcgacacgt cggttgcatt ttaccgattg cgtcgatcgt
     7141 taactaagcc atccaaacta ggtggcgttg atcccgctat tttgggtggt tttaactcaa
     7201 gtctatgtcc gatcgttcgt tgacggcggg acgcctgttc ggtggccgct gttccgtgtc
     7261 gaacgaagga cgaagatggg cgatcaagat tccggcgaca cttgccaggg ggacatgatc
     7321 gagcggcggc gaatccctgc atcgcggtca aggctttacc ggcccgcgct ggttgcagcc
     7381 ggcatcggcc ttctgctgtc attggccggg gcatctgcgg tcggacagtg ggaagagcgg
     7441 gtcacgaaag tcgagtttga gggcgtagcc gagactcagg cgatcatcat gcagaacggc
     7501 atgaacgaat acgtcagccg gctggctgcg ctgcgtaccc tgttcgaatc cgccaacgag
     7561 gaaatcaccc gcagcgagtt cgagaccttc agtgggcgcc tgttcgagcg acatccgggc
     7621 atccttcgca tcggttggat cccgaggatc aaccggaagg agcgcgccga atacgaagct
     7681 gccgcggttg gcgacggaat ttccggctat cgaatcaaat cgctccttgc ggacggcggc
     7741 attgcgacgg ctccgcagag cgatgaatat tttccgattt tcttttccac ggaaccgaaa
     7801 acctcggtgg cttacgggct ggattattcg accgatcccg cccggcgggc tacgctggaa
     7861 cgcgcgcggg acaacgatct ggtcgcgacg ttgcgcgcgc cactttacga gccgaaaaac
     7921 ggtatccggc ccctgggcgt gctggtttgt gtgccggtct acgtaaaagg aacttcgcgt
     7981 gccacgatcg ccgaccggcg gcgcaatctg gccggctttg tcgtcggggt attcaatctg
     8041 cggctattgc tggagtcgat tcgcacgacg acggcggcaa cccccgccgt cagcatcaat
     8101 gtctacccgt ccgatatggg ccgcagcgcg ggaccggaaa accggttcgt tcccgatttt
     8161 tcgtccgagc ccggcgcgtc gcagtcggcc ttcgcggatg ggccgcactg gtccggcact
     8221 ctcaagatag ccgatgccag ctggcacgtg cgggtcactc ccaccgccga cggccggctg
     8281 acgacgcgtc acgaacgagc tctgaccgtg ctggcggccg gaattgtcat cacgacgttc
     8341 ctggcggtgt atctcggtct cgcaagccgc aactcgcgcc agctcgcact ggcgaaccgg
     8401 cgggttctgg agcttgcgca gaccgacatt ctgaccgggc ttccgaaccg ggcgttcttt
     8461 ctggaacaat tggaggacgc cgactcgtac gagcgccggg gcagcggggc gttttcaatc
     8521 ctcatgctcg atctcgatcg cttcaagaac gtcaacgact cgcttggtca tgccgccggc
     8581 gacgcgttgc tgcggcaggt ggcgcttcgt ttgaaatccg cgatacgcag taccgatgtg
     8641 ctggcgcggc ttgggggcga cgaattcgca atcatccagg cagagtgcga cgatcaacgg
     8701 accggttcga tcgatctggc gacccgaatc tccaaactga tcgccgagcc attcctgctg
     8761 cagggcaatc aggtggaagt cggtaccagc atcggaatct cgatggcgcc ggagcacggc
     8821 agcgatcagg agcagttgtt gaaaaaagcc gaccttgccc tttatcgatc gaaatcggcc
     8881 ggccgcaact gcttcaccct ttatgatgaa gcgatgtccg cggaactgga agcccgaaac
     8941 acgctggaga gcgacttgcg ggacgccgtc gctcgctgtc agttcgagtt acattaccaa
     9001 ccgttcttcg atgtccagac cggccagcgg cgtggcctgg aagcgcttgt tcgctggcgt
     9061 catccgacca gagggctgat accgcccgac cagttcatcc cgctcgctga ggagaccgga
     9121 ctcatcgttc cgcttggtga atgggtgctg cggcgagcct gcgacgacgc gacatcctgg
     9181 ccggcagaca tcaaggtcgc ggtgaacttg tcaccggtcc agctcaagca ggtcgagttg
     9241 tttgatgtca tccagtccgc gcttcggagt tccggtttgc cgccggagcg gctggaaatt
     9301 gaactcactg aatccgtgct gctggaacgg gcggtcgaga atcatgcgtt gatggaacga
     9361 ctgaagagta tcgggatttc gctcgcgctt gacgacttcg gcaccggata ttcatcgcta
     9421 agctgcctga cggcgttccc gttcgacaag atcaagatcg acaggtcgtt catcggcaat
     9481 ctgaccaagc gtcacaagag ttcggcgatc atatcgtcga tcgtgaccct cgcgcgcggc
     9541 ctgaacctgt cggtcactgc cgaaggtgtg gaaaccagcg agcaattcga gaggctgaaa
     9601 acgctggggg tgaacttcgc tcagggctac ctgctcggcc ggccgatgcc gctcggcgac
     9661 ccgaaggtca gtctcaatca gatcatgcgc acctgggtgc agctttgcgt cagtagtcgc
     9721 gccgtggcgt cgggatgagc gcagcggcca aacagcgccc gcctactttc tcacgcagat
     9781 cacgcgcagg acggacgcct ttacctccga cgatccatcc gccgcattca ccgcattggc
     9841 tttgagaaac tcgcgcacgg tatcggccga taccagcgtc gcctgggccg cgggcgcggc
     9901 attcggcggt cccgcgacga ccaccgactt gagcagcgca atgccggcga atttgccgtc
     9961 ggcatcgaac gccgccgcgc cggaaaagcc gaccgccggc gccggcgcca gcgccagatc
    10021 gttgccgctg ccgacttgcg tggtggttgc cttgacgctg ctcgccgcgg caccgccgcc
    10081 ctggttctgt ggatcggcaa cgcctgtcag ctcgacgctg gttttcgccg cgccattgcc
    10141 gaggccgagc ggcttcaatc cgcgcgcgcc gtagatgcgc agcagtgcca gatcatggtc
    10201 cttgtcctcg gcgacgcgat cggcgttgcc aaagcctgcg atcgcgatgg agaggcagcc
    10261 gtcggtgatc tggcggtcgg tcacgatcgc gccgtcgtcg ctcaccacaa tgccggtgcc
    10321 gtattcgacg gtcttgcgcg gcggcggccc ggcgatctgg gcgcccgatg gaaacggatt
    10381 gaacgcgctc gacatcgcga tcacgatcgg ttcaacggtg ttttctgtag cctggtcgta
    10441 gagaatggtg aggatgcgga cctcgtcgcc cttgaacgtg ccgcgcacat agaatttctt
    10501 cagaccctgc aggcccgaca gcacgaagaa gtccggcttg accacggtgt agtcgacggc
    10561 gcgcccggcg ggctctttct tttccttctc ggcgagtttt gccgtggtgg gattggcctc
    10621 cttgcgtcgt gcaagctgaa tctggatggt ggcggtgggg gaactccacc tggcgccgtt
    10681 ggcgtcagag gattgctgcg gtaccagctt ggtcgggatg ccgagccgca ccccgctgcc
    10741 ggcatcggtg acgattttcc agccgacatt gtcctgccgc cgcctggcgg tttcggcgag
    10801 cgcgccgcgc tcctgcggat tcagcacacc ggtcggcttg ccgccgcgtt ccttctgaaa
    10861 ctcc
//
LOCUS       DASWGM010000737         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1765765,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000737 DASWGM010000000
VERSION     DASWGM010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765765"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VGA15_20615"
     CDS             complement(<1..698)
                     /locus_tag="VGA15_20615"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX9590137.1"
                     /translation="MKSPICDMLGIEFPLLAFSHCRDVVAAVSRAGGFGVLGATSHSP
                     ESIEQELKWIDDHVDGKPYGLDVLIPENISTAGEKDVTWKSLELRISPEHRAFTKNLL
                     KEYGIELTTTSVADNQPQPFDAQRALEVLEASFRHPIRLIANALGVPPKAMIEMGRQH
                     GVPVAALVGAKEHALRQVAAGVDILVVQGTEAGGHCGEVSTMVLVPEVIKAIKPIRDV
                     PVLAAGGIMTGRQMA"
     gene            1177..>2844
                     /locus_tag="VGA15_20620"
     CDS             1177..>2844
                     /locus_tag="VGA15_20620"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9590138.1"
                     /translation="MSALQSVAGKSHAAGRETYDVVVVGAGFAGMYMLHKMRGLGLSV
                     RVYEQGGDVGGTWYWNRYPGARCDVESMQYSYSFSDELQQEWDWSERYAPQPEILKYA
                     NHVADRFDLRSDIQLDTRVDRAVFDESTNVWLVATSDGMTVTAKYVVLATGCLSNARM
                     PDIKGLTDFKGKVYHTGHWPHERIDFTGQRVGVIGTGSSAIQSVPIIAEQASHLTVFQ
                     RTANFSIPARNAALSAEERQSWRADYPEIRRKAREETRNGIFTEVPDKGALDDGDNER
                     RAKYEQRWTRGGLTFMSVYNNLALDKAANDTAADFVRDKIAEIVKDPHTARLLQPNNH
                     PIGSKRICIDSDYFATFNRPNVTLVDIKSNPIEEILPNAVRTGEKDYEVDALVLATGF
                     DAMTGSVAKIDIRGRNGQTLNQKWAEGPKTYLGLMSAGFPNLFIITGPGSPSVLSNMI
                     VSIEQHVDWIADCLAWMRDRDLVTMEAKKDAEDSWVAHVNEVAHTTLYPQANSWYMGA
                     NIPGKPQIFMPYIGGVAIYRQTCNDVAAKGYEGFTMSVAERPRKLAAS"
BASE COUNT          564 a          887 c          865 g          528 t
ORIGIN      
        1 gccatctggc gcccggtcat gatgccgccc gccgccagca ccggcacatc gcggatcggt
       61 ttgatggctt tgatcacctc gggcaccagc accatggtgg aaacctcgcc gcaatggccg
      121 ccggcctcgg tgccctgcac caccagaata tcgacgccgg ccgcgacctg acgcagcgcg
      181 tgttccttgg cgccgaccag tgctgcgacg ggaacgccgt gctgcctgcc catttcgatc
      241 atcgccttcg gcggcacgcc cagcgcattg gcaatcaagc ggatcgggtg ccggaacgaa
      301 gcttcgagaa cttccaacgc gcgttgcgcg tcgaacggct gcggctgatt gtcggcgacg
      361 ctggtcgtcg tcagctcgat gccgtattcc ttcagcaggt tcttggtgaa ggcgcgatgc
      421 tcgggcgaga tcctcagctc caggcttttc caagtgacgt ccttctcgcc cgccgtcgag
      481 atgttctccg ggatcagcac gtcgagccca tagggcttgc cgtcgacgtg atcgtcgatc
      541 catttcagtt cctgctcgat cgattcaggc gaatgcgagg tggcgccgag cacgccgaaa
      601 ccgccggcgc ggctgaccgc tgccaccacg tcgcggcagt ggctgaacgc cagcaggggg
      661 aattcaattc ccagcatgtc gcagatcggc gatttcatgt tttgtctgct ccccgcggca
      721 gcgtttggcg ctgctcatta ttggtttgaa tgacgaattc tccaacgacg ctagcgcatc
      781 atgggaggga aagccaagcg ggccttcgtc cctctccctg ctctgcgcgg ggagagggtg
      841 cctgagcgaa gcgagggcgg gtgaggggcg aggcattggg ttggccacgt tcaccaacct
      901 catcggtcag cacatctagc agaggcatgc ggcagctcgg ttgaaccagt tcgggaccag
      961 cgaggcagtt accatattga ttcagtgccc ggcccttcac ccggatcgca aatgcgatcc
     1021 gacctctccc cgcaaagagc ggggagaggt tagtgcaacg cattccgcgt ggcaaaatat
     1081 gcggcccgga ccggccgttt tgtctcttgc gctttcgcgc ggcgggtaga atgctggcca
     1141 gcaatacaac aaagaatccc aagggagcgc gaagacatgt ccgccttgca atccgttgcc
     1201 ggcaaaagcc acgccgcagg cagagagacc tacgatgtcg tcgtggtcgg cgcaggcttt
     1261 gccgggatgt acatgctgca taaaatgcgc gggctgggat tgtcggtccg ggtctacgag
     1321 cagggcggcg atgtcggcgg cacctggtac tggaaccgat atcccggcgc acgctgcgat
     1381 gtcgaaagca tgcagtattc ctattcgttc tcggacgagc tgcagcagga gtgggactgg
     1441 agcgagcgct atgcgccgca acccgaaatc cttaaatacg ccaaccatgt cgccgaccgt
     1501 tttgatctcc gcagcgatat ccagctcgac acccgcgtcg accgggctgt gttcgacgag
     1561 agcaccaatg tgtggctggt cgccacttcc gacggcatga ccgtcacggc aaaatatgtc
     1621 gttctcgcca ccggttgcct gtcgaatgcg cggatgccgg atatcaaggg cctcaccgat
     1681 ttcaagggca aggtctatca caccggccac tggccgcatg aacggatcga tttcaccggc
     1741 cagcgcgtcg gcgtgatcgg caccggatca tccgcgattc agtcggtgcc gatcatcgcc
     1801 gagcaggcca gccatctcac ggtgtttcag cgcaccgcga atttttccat acccgcccgc
     1861 aacgcggcgt tatcagcgga ggagcggcaa tcctggcgcg cggactatcc ggaaatccgg
     1921 cgcaaggccc gcgaagagac ccgcaacggc atttttaccg aggtgccgga caagggcgcg
     1981 ctcgatgacg gcgacaacga gcgccgcgcc aaatatgagc agcgttggac ccgcggcggc
     2041 ctcactttca tgtcggtcta caacaatctg gcgctcgaca aggccgccaa cgacaccgcc
     2101 gccgatttcg tccgcgacaa gatcgcggaa atcgtcaagg atccgcacac cgcaaggctg
     2161 ctgcagccca acaatcaccc gatcggctcg aaacgcatct gcatcgattc cgattatttt
     2221 gccaccttca accgtccgaa cgtgacgctg gtcgacatca agtccaatcc gatcgaggag
     2281 atattgccga acgccgtgcg caccggggaa aaggattacg aggtcgatgc gctggtgctg
     2341 gccaccggct tcgacgccat gacgggttca gttgcaaaga tcgatattcg cggccgcaat
     2401 ggccagacgc tcaaccagaa atgggcggag ggtccgaaaa cctatctcgg cttgatgagc
     2461 gcgggctttc ctaatctctt catcatcacc gggccgggca gcccgtcggt gctgagcaac
     2521 atgatcgtct cgatcgagca gcacgtcgac tggatcgccg attgcctcgc ctggatgcgc
     2581 gaccgcgatc tcgttaccat ggaagccaaa aaggacgcgg aggacagttg ggtagcgcac
     2641 gtcaacgagg tcgcgcatac cacgctctat ccgcaggcca attcctggta catgggcgcc
     2701 aatatcccgg gcaagccgca gatcttcatg ccctatatcg gcggcgtcgc gatctatcgt
     2761 cagacctgca acgacgtcgc ggcgaaaggc tatgaggggt ttacgatgtc ggtcgcagag
     2821 cggccgcgga agctggccgc gtct
//
LOCUS       DASWGM010000738         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1035208,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000738 DASWGM010000000
VERSION     DASWGM010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035208"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..985)
                     /locus_tag="VGA15_20625"
     CDS             complement(146..985)
                     /locus_tag="VGA15_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEX9590139.1"
                     /translation="MLPKPVLVAQISDLHIKSPGQLAYGRVDTAKALERCITALNEFR
                     PRPDLVVISGDLADTPTADEYEHLKCLLSQLDPRFVSIPGNHDSRKLMRAAFPHAAYA
                     FSSGPLNQRVEIGGLDLVLLDSSVAGKPHGELDVPTLQWLEAMLASATDRPALLFLHH
                     PPFKTGIWQMDRQNLLNASEFEPILRRYPRVQLIATGHVHRATLTMFAGVPTTICPAP
                     NHAVDLDLEELREPSFRVEPPAFHLHSWFPGEGFGSVVTHQVPIGDFDGPHPFFGPDG
                     KLL"
     gene            complement(996..>1925)
                     /locus_tag="VGA15_20630"
     CDS             complement(996..>1925)
                     /locus_tag="VGA15_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590140.1"
                     /translation="PSGCGKTTMLRIIAGLELPDAGGRVLFDDKDMTSVPIEKRNVGM
                     VFQSYALFPNMSVADNIGYGLKIRGMARQERAARVAELVVLTNITGLERRRVDQLSGG
                     QRQRVALARAVAIRPGILLLDEPLTALDAALRDRLRGELNRLLRALGITTIYVTHDQS
                     EAMELGDRIVVMRKGAIAQMGTPREIYFTPKNRFVAEFIGAANIIEAPVENGHLVLPG
                     GRQPIGGDASRAAAVAMIRPETIRVVEAGGAPLSGIIDSVSFIGDRQRMIVGGASDKL
                     LTVDAPNTVRAQAGERIGLLIAPDAVRLLPPEN"
BASE COUNT          374 a          589 c          606 g          356 t
ORIGIN      
        1 tgatcggcgg gggcgacaga gggcgtgcat cccaaatgat ggagatgtac gccctccaac
       61 cggacaggag tgtccaggcg agccgcgcca acttacctct ccaacggagc caatttcagc
      121 gttggaagag caccggtcgg cgcactcaca gcagcttccc gtccggcccg aaaaacggat
      181 gcggtccgtc gaaatcgccg atcggcactt gatgcgtcac cacgctgccg aatccctccc
      241 ccggaaacca gctgtggaga tgaaaagccg gcggctcgac cctgaacgac ggctcgcgca
      301 attcttccag atccagatcg accgcgtgat ttggcgccgg gcagatcgtc gtcggcacgc
      361 cagcgaacat cgtcagggtc gcgcgatgaa cgtgacccgt ggcgatcagt tgcacgcgcg
      421 gatagcgtct gaggatcggc tcgaattcgc tggcattgag caggttctgg cggtccatct
      481 gccagatgcc ggttttgaac ggcggatggt gcaggaacaa tagcgccggc cggtcggttg
      541 cggatgccag cattgcttca agccattgca gggttggcac atcgagttcg ccatgcggtt
      601 tgccagctac gctggaatcc agcagcacca gatcgagccc gccgatttcg acgcgttgat
      661 tgagcgggcc ggatgaaaag gcataggcgg cgtgcggaaa tgccgcgcgc atcaatttcc
      721 gtgaatcgtg attgccggga atgctgacaa atcgcggatc gagctgcgac agcaagcact
      781 tgaggtgttc gtattcgtcg gccgtcggcg tatcggcgag atcgcccgat atcaccacga
      841 ggtcgggtct tggcctgaac tcattcaagg cggtgatgca gcgctcaagt gccttcgccg
      901 tatcgacacg cccgtaagcc agctggcccg gcgatttgat gtgcagatcg gaaatctgcg
      961 caacgagaac tggttttggc aacatttgag cttcttcaat tttccggtgg cagcaggcgg
     1021 acggcgtccg gcgcgatcaa aagtccgatc cgttcgccgg cctgggctcg aaccgtgttc
     1081 ggcgcatcga cggtaagaag cttgtcggac gcgccaccga cgatcatccg ttgcctgtcg
     1141 ccgatgaaac tgacgctgtc gataatcccc gagagcggcg cgcctccggc ctcaacaacc
     1201 cggatcgtct cggggcggat catggcgacc gcggccgcgc gactggcgtc accgccgatc
     1261 ggttgccggc cgcccggcag cacaagatga ccgttttcga ccggggcttc aataatgttc
     1321 gcagcgccaa tgaactcggc cacgaaacga tttttaggcg tgaagtaaat ctcgcgcggc
     1381 gtgcccatct gggcaatcgc gcccttccgc atgacgacga tgcggtcgcc gagttccatg
     1441 gcctcggact gatcgtgagt cacatagatc gtggtgatgc cgagcgcgcg cagcaaacgg
     1501 ttgagttcgc cacgcaggcg gtcgcggagt gcggcatcga gcgcggtcaa gggctcgtcg
     1561 agcagcagga tgcccggccg gattgccacc gcgcgtgcca gcgcaacacg ctggcgctga
     1621 ccgccggaga gctggtcgac gcgtctcctc tcgagcccgg tgatattggt cagcaccacc
     1681 agctcggcga cgcgcgccgc ccgctcctgc ctcgccatgc cccggatttt caggccatag
     1741 ccgatgttgt ccgcgacgct catattggga aacagcgcat aggactgaaa caccatgccg
     1801 acgttgcgct tctcgattgg taccgaagtc atgtccttat catcaaacag tacccggccg
     1861 ccggcatcgg gcaattcgag gcctgcgatg atgcgcagca tggtggtttt tccgcaaccg
     1921 gaggg
//
LOCUS       DASWGM010000739         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2311203,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000739 DASWGM010000000
VERSION     DASWGM010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2311203"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VGA15_20635"
     CDS             <1..298
                     /locus_tag="VGA15_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9590141.1"
                     /translation="SGRIDPDGNSYIFHKSKAPQNYSAYSNPDVDRLLDDARLVTDMA
                     QRKSIYEKMTKSILEDEPIIYLYHRKNLIVHTTKLEGYKQLPDGLVRVVGLKLK"
     gene            444..1385
                     /locus_tag="VGA15_20640"
     CDS             444..1385
                     /locus_tag="VGA15_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590142.1"
                     /translation="MLSFLAKRLVQLIPTLFFVSILIFSLQHLLPGDPALVMAGEERD
                     PDVIAHIRQQYRLDQPIPVQYIYWVKGVLSGDFGESLRIKVPVRELIAQKLPVTMQLA
                     SIAIVIAFLIGIPAGIVSAVKKGTLRDYGANLFALWGISTPNFWLGIMLIFLFSIELG
                     WLPASGYVPLSENWRASLASTIMPAFVLGNAIAAILMRHTRSAMLQVLESDYVRTARA
                     KGLSERSVILKHAMRNALTPVITLGALELGTLLSGAVLTEQIFSIPGFGKLIVDAVFN
                     RDYAVVQGVVLVTATIYITLNLIADIAYILVNPRLRA"
     gene            1389..2282
                     /locus_tag="VGA15_20645"
     CDS             1389..2282
                     /locus_tag="VGA15_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590143.1"
                     /translation="MTDAALTTIRLADGRANELESPARRALRRVLQRKGAVVGLIVIA
                     TFIMLALFAPWIVPYDPIATSWTLVRKPPSALHWFGTDDLGRDILGRVIYGARASLMA
                     GAISVGIALGVGVPFGLLSGYRGGFIDALISRITDAMLACPFLILAIALAAFLGPSLG
                     NAMIAIGISTTPIFVRLTRGQVMTVKVEDYVEAARAMGNPRWRIALFHILPNIMPALL
                     VQATLSIAAAIIAEAALSFLGLGQQPPAPSWGSMLNAAQRFLTNAPWMALWPGLAIFL
                     VVLSFNLVGDGLRDALDPRQR"
     gene            complement(2324..2515)
                     /locus_tag="VGA15_20650"
     CDS             complement(2324..2515)
                     /locus_tag="VGA15_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590144.1"
                     /translation="MPVKIFYAQGNKNIGGLEAEINKWQESLGPRASVTHTSTAMAEH
                     NDDVNGRETRVIVTIWYEL"
BASE COUNT          466 a          854 c          822 g          577 t
ORIGIN      
        1 gagcggccgc atcgatcccg acggcaactc ttatattttc cataagtcca aggcgcccca
       61 gaattatagc gcctattcca acccggacgt cgacaggctg ctagacgacg cgcgcctcgt
      121 gaccgacatg gcgcaacgca aatcgattta cgagaagatg acgaaatcga ttcttgagga
      181 cgagccgatc atctatctct atcaccgcaa gaacctgatc gtgcacacca ccaagctcga
      241 aggctacaag cagctgccgg acggattggt gcgcgtggtc gggttgaaac tgaagtgagg
      301 agcctgcata tgctctcgtg tcccggacgc gatgcagcgt tcttacgctg cttcgcagag
      361 ccgggaccta ccgcgatgag atgggtcccg gttctgcggc gcaacgcttc acgatgcgcc
      421 gcgcccggga cacgagaaac gccatgctga gctttctcgc caagcggctg gtccagttga
      481 tcccgacgct gtttttcgtg tcgatcctga tcttctcgct gcagcaccta ttgcccggcg
      541 acccggcact ggtgatggcc ggcgaggaac gtgatcccga tgtcatcgcc catatccgcc
      601 agcaataccg gctcgatcaa ccgataccgg tgcaatacat ctattgggtc aagggcgtat
      661 tgtccggcga cttcggcgag tcgcttcgca tcaaggtgcc ggtgcgcgag ctgatcgcgc
      721 aaaaactgcc ggtgacgatg caactggcgt cgatagcgat cgtgatcgcg tttctgatcg
      781 gcattcccgc tggcatcgtg tcggcggtga agaagggcac gctacgggac tacggcgcca
      841 atctgtttgc gctgtggggc atctcgacgc cgaatttctg gctcgggatc atgctgatct
      901 ttctgttctc gatcgagctc ggctggctgc cggcatcggg ctatgtccct ctctccgaaa
      961 actggcgcgc cagccttgca tccaccatca tgcccgcctt cgtgctcggc aatgccatcg
     1021 ccgcgatcct gatgcggcac acccgcagcg ccatgctgca ggtactcgaa agcgattatg
     1081 tccgtaccgc ccgcgccaag ggcctttccg aacgctcggt cattctcaag cacgccatgc
     1141 gcaacgcgct gacgccggtc atcacgctcg gcgcgcttga actgggcacg ctgctgtcgg
     1201 gcgcggtgct gaccgagcag attttttcga ttccgggttt tggcaagctg atcgtcgacg
     1261 cggtgttcaa tcgcgactat gccgtggtgc aaggcgtggt gctggtcacc gccaccatct
     1321 acatcacgct gaacctgatc gccgatatcg cctatatcct ggtcaatcca aggctgaggg
     1381 cgtaggccat gaccgacgca gcgctcacca cgatccggct tgccgatgga cgcgccaatg
     1441 agcttgaaag cccggcgcgg cgcgcgctgc gccgggtgtt gcagcgcaag ggcgcggtag
     1501 tcgggctcat cgtgatcgca accttcatca tgctcgcgct gtttgcaccc tggatcgtgc
     1561 cctatgaccc gatcgcgacc agctggacgc tggtgcgcaa gccgccctcg gcactgcact
     1621 ggttcggcac cgacgacctc ggtcgcgata ttctcggacg cgtgatctat ggcgcgcgcg
     1681 cctcgctgat ggcgggcgcg atctcggtcg ggatcgcgct tggggtcggc gtgccgttcg
     1741 gactattgtc ggggtatcgc ggtgggttca tcgacgccct gatcagccgc atcaccgacg
     1801 ccatgctggc ctgcccgttc ctgatccttg ccatcgcgct ggcggcgttt ctgggcccga
     1861 gccttggcaa cgccatgatc gccatcggca tttcgacgac gccgatcttc gtgcggctga
     1921 cccgcggcca ggtgatgacc gtcaaggtcg aggactatgt cgaggccgcc cgcgcgatgg
     1981 gcaatccgcg ctggcggatc gcgctgtttc atattctgcc gaacatcatg ccggcgctgc
     2041 tggtgcaggc gacgctgtcg atcgcggcag cgatcatcgc cgaagcggcg ctgtcgttct
     2101 tgggcttagg ccagcagccg ccggcgccgt cctggggcag catgctcaac gccgcgcaac
     2161 gatttctgac caacgcaccg tggatggcgc tgtggccggg gcttgcgatt tttctcgtgg
     2221 tgctgtcgtt caatctggtc ggcgacggcc tgcgcgacgc gctcgatccg cgccagcgct
     2281 gagcgtcgag aaccagtgat ttcctgatct gaatttcggc cgatcataat tcgtaccaga
     2341 tcgttacgat cacgcgcgtc tcccggccgt ttacgtcgtc gttatgctct gccatggccg
     2401 tcgatgtatg ggtaacggag gctctggggc caaggctttc ctgccatttg ttgatttctg
     2461 cctccaatcc cccgatattc ttgttgccct gcgcataaaa gattttgact ggcatttctg
     2521 ctctcccttg cggttcgatg ttcgtcgtgc ggcgcgcacg ggaaatattc tagatcaccg
     2581 tctcgcgcat gacgcggcgg caatccatct cgtattcgag gtcgatcacc ggcgggcgcg
     2641 caaaatgcca ggtcaggccg aaacgcgtgg cgccgcggcg gaccagttca tcggacatca
     2701 gcggatgaaa gtcgcgaac
//
LOCUS       DASWGM010000740         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2455906,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000740 DASWGM010000000
VERSION     DASWGM010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2455906"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3653
                     /gene="rpoB"
                     /locus_tag="VGA15_20655"
     CDS             <1..>3653
                     /gene="rpoB"
                     /locus_tag="VGA15_20655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX9590145.1"
                     /translation="KVTLRLIVFDIDEETGAKSVKDIKEQDVYMGDIPLMTMNGTFVV
                     NGTERVIVSQMHRSPGVFFDHDKGKTHSSGKLLFAARVIPYRGSWLDIEFDAKDIVFA
                     RIDRRRKIPVTSLMFALGLDGETILSTFYKKISYKRTKDGWRVPFDANRFRGYSTIND
                     LIDADSGKVVLEAGKKLTVRAARQLQEKGLKALRLSDEELVGNYLAEDLVNPKTGEIY
                     AEAGEEITEKSLKALNEHGYKELPLLDIDHVNVGAYIRNTLAADKNMTREDALFDIYR
                     VMRPGEPPTIDSAQTMFQSLFFDSERYDLSAVGRVKMNMRLELDAPDTHRTLRKEDIL
                     AVIKTLVGLRDGKGEIDDIDHLGNRRVRSVGELMENQYRIGLLRMERAIKERMSSVDI
                     DTVMPQDLINAKPAAAAVREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRER
                     AGFEVRDVHPTHYGRICPIETPEGPNIGLINSLATFARVNKYGFVETPYRKVKDGRVT
                     DEVVYLSAMEEGRYSVAQANVALDAKGRFTEDLIVCRDGGTRDVLLIPADKVNYMDVS
                     PKQLVSVAAALIPFLENDDANRALMGSNMQRQAVPLVRAEAPFVGTGMEGVVARDSGA
                     AIAARRTGVIDQIDATRIVIRATEDLDPTKSGVDIYRLMKYQRSNQSTCINQRPLVKV
                     GDLVHKGDIIADGPSTDLGELALGRNVLVAFMPWNGYNFEDSILLSERIVKDDVFTSI
                     HIEEFEVMARDTKLGPEEITRDIPNVSEEALKNLDEAGIVYIGAEVRAGDILVGKITP
                     KGESPMTPEEKLLRAIFGEKASDVRDTSLRVPPGVQGTIVEVRVFNRHGVDKDERALA
                     IEREEIERLAKDRDDEQAILDRNVYGRLAELLENRQGIAGPKGFKKDTKITRAVLDEH
                     PRSQWWMFASPNDKLMAEIEAMRKQYDESKRGLEQRFLDKVEKLQRGDELPPGVMKMV
                     KVFVAVKRKIQPGDKMAGRHGNKGVVSKIVPIEDMPFLEDGTHADIVLNPLGVPSRMN
                     VGQILETHLGWACAGLGKRIGQAVDAYLASAKQDTKPLKETLKKIYGDEETIKSLNDA
                     ELMELGGNLRHGVPIATPVFDGAKEADIEEMLKLAGLDASGQSTVYDGRTGDAFDRKV
                     TVGYIYMLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEMLTVKSD"
BASE COUNT          755 a         1137 c         1139 g          622 t
ORIGIN      
        1 tgaaggtgac gctgcgtctc attgtgtttg atatcgacga agagaccggc gccaagtcgg
       61 tgaaggacat caaggagcag gacgtctaca tgggcgatat cccgctcatg accatgaacg
      121 gcaccttcgt cgtcaacggc accgaacgcg tcatcgtctc gcagatgcat cgttcgcccg
      181 gcgtgttctt cgaccacgac aagggcaaga cccattcgtc gggcaagctg ctgtttgccg
      241 cgcgcgtaat tccctatcgc ggctcctggc tcgatatcga attcgacgcc aaggacatcg
      301 tgttcgcgcg tatcgaccgc cgccgcaaga ttccggtgac ctcgctgatg ttcgcgctcg
      361 ggctcgacgg cgaaaccatc ctttcgacgt tctacaagaa gatcagctac aagcgcacca
      421 aggatggctg gcgcgtgccg ttcgacgcca accgtttccg cggctacagc acgatcaacg
      481 acctgatcga tgccgacagc ggcaaggttg tgctcgaagc cggcaaaaaa ttgaccgtgc
      541 gcgccgcccg tcagctgcag gaaaagggcc tcaaggcgct gcgcctgtcg gacgaagagc
      601 tggtcggcaa ttaccttgcc gaagacctcg tcaatccgaa gaccggtgaa atctacgccg
      661 aggccggcga ggaaatcacc gagaagtcgc tgaaggcgct caacgagcac ggctacaagg
      721 aattgccgct gctcgacatc gaccatgtca atgtcggcgc ctatatccgc aacacgctcg
      781 ccgccgacaa gaacatgacg cgtgaggacg cgctgttcga catctatcgc gtgatgcgcc
      841 ccggcgaacc gccgaccatc gactcggccc agaccatgtt ccagtcgctg ttcttcgact
      901 ccgagcgcta cgacctgtcc gcggtcggcc gcgtcaagat gaacatgcgc ctcgaactcg
      961 acgcgcccga cacccatcgc accctgcgca aggaagacat cctcgccgtc atcaagacgc
     1021 tggtcggcct gcgcgacggc aagggcgaga tcgacgacat cgatcacctc ggcaaccggc
     1081 gcgtgcgctc ggtcggcgag ttgatggaga atcagtatcg tatcggtctg ctccgcatgg
     1141 agcgcgcgat caaggagcgc atgtccagcg tcgatatcga caccgtgatg ccgcaggacc
     1201 tgatcaacgc caaaccggcg gccgccgcgg tgcgcgagtt cttcggctcg tcgcagctgt
     1261 cgcagttcat ggatcagacc aatccgctgt cggagatcac ccacaagcgt cgcctctcgg
     1321 cgcttggccc gggcggtttg acccgcgagc gcgccggctt cgaggtgcgc gacgtgcatc
     1381 cgacgcatta cggccggatc tgcccgatcg agacgccgga aggcccgaac atcggcctga
     1441 tcaactcgct cgcgacgttt gcgcgcgtca acaaatacgg cttcgttgaa acgccctatc
     1501 gcaaggtcaa ggacggccgc gtcaccgacg aggtggtcta tctctcggcg atggaggagg
     1561 gacgttattc cgtcgctcag gccaatgtgg cgctcgacgc caagggccgc ttcaccgagg
     1621 atctgatcgt ctgccgagac ggtggcaccc gcgacgtgct gctgatcccg gccgacaagg
     1681 tcaactacat ggacgtgtcg ccgaagcagc tggtttcggt ggcggcggcg ttgattccat
     1741 tcctcgagaa cgacgacgcc aaccgcgcgc tgatgggctc gaacatgcag cgccaggccg
     1801 tgccgctggt gcgcgccgaa gcgccgttcg tcggcaccgg catggaaggt gtggtcgcgc
     1861 gcgactcggg agccgcgatc gcggcccgcc ggaccggcgt gatcgaccag atcgacgcca
     1921 cccgtatcgt gatccgcgcc accgaggatc tcgatccgac caagtcgggc gtcgatatct
     1981 accggctgat gaaatatcag cgctccaacc agtcgacctg catcaaccag cgtccgctgg
     2041 tcaaggtcgg cgacctcgtg cacaagggcg acatcattgc cgacggtccc tcgaccgatc
     2101 tcggcgaact cgcgctcggc cgcaacgtgc tggtcgcgtt catgccgtgg aatggctaca
     2161 acttcgaaga ttcgatcctg ctgtcggaaa gaatcgtgaa ggacgatgtc tttacctcga
     2221 tccacatcga ggaattcgag gtgatggccc gcgacaccaa gctcggcccc gaggaaatca
     2281 cccgcgacat tccgaacgtc tcggaagaag cgctgaagaa cctcgacgaa gccggcatcg
     2341 tctatatcgg cgccgaagtg cgtgccggcg atatcctggt cggcaagatc acgccgaagg
     2401 gcgaaagccc gatgacgccg gaagaaaagc tgctgcgcgc gatcttcggc gaaaaggcat
     2461 ccgacgttcg cgatacctcg ctgcgcgtgc ctccgggcgt gcagggcacc atcgtcgaag
     2521 tccgcgtctt caaccggcat ggcgtcgaca aggacgagcg cgctttggcg atcgaacggg
     2581 aagagatcga acgtctggcc aaggaccgcg atgacgagca ggcgatcctc gaccgcaacg
     2641 tctatggccg tctggccgag cttctggaga accgccaggg catcgccggt ccgaagggtt
     2701 tcaagaagga caccaagatc acgcgcgcgg tgcttgatga gcatccgcgg tcgcaatggt
     2761 ggatgttcgc gtcgcccaac gacaagctga tggctgaaat cgaggcgatg cggaagcagt
     2821 acgacgaatc gaagaggggc cttgaacagc gcttcctcga caaggtcgag aagctgcagc
     2881 gtggcgacga attgccgccc ggcgtgatga agatggtcaa ggtcttcgtc gcggtgaagc
     2941 gcaagattca gcccggcgac aagatggcgg gccgccatgg caacaagggc gtggtgtcca
     3001 agatcgttcc gatcgaggac atgccgttcc ttgaggacgg tacccacgcc gatatcgtgc
     3061 tcaatccgct cggcgtgcct tcgcgcatga acgtcgggca gattctcgaa acccatctcg
     3121 gctgggcctg cgccggcctc ggcaagcgca tcggccaggc ggttgatgcc tatcttgcca
     3181 gcgccaagca ggacaccaag ccgctgaagg agaccttgaa gaagatctac ggcgacgagg
     3241 agaccatcaa gtcgctcaac gacgccgaat tgatggagct tggcggcaac cttcgccatg
     3301 gcgtgccgat cgcgacgccg gtgttcgatg gtgccaagga agccgacatc gaggagatgc
     3361 tgaagcttgc cggtctcgac gcctccggac aatcgaccgt ttacgacggc cggaccgggg
     3421 atgcgttcga ccgcaaggtg acggttgggt acatctatat gctcaagctg catcaccttg
     3481 tggacgacaa gatccacgca cgttcgatcg gaccgtactc gctcgtgacc cagcagccgc
     3541 tgggtggcaa ggcgcagttc ggcggccagc gtttcggcga aatggaagtg tgggcgctcg
     3601 aggcttacgg cgcggcgtac acgctccagg aaatgctgac cgtgaagtcg gac
//
LOCUS       DASWGM010000741         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2595951,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000741 DASWGM010000000
VERSION     DASWGM010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595951"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VGA15_20660"
     CDS             complement(<1..1230)
                     /locus_tag="VGA15_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX9590146.1"
                     /translation="MSETLETLVPSQATPNPLLMAWQTPFETPPFAEIAPEHFLPAFE
                     QAFADHTAEITAITYDPSVPDFANTITALERAGKLLSKVSAVFYDLVSAHSNPTLLEI
                     DKEVSLRMARHWNPIMMNAVLFGRIALLHGNQATLGLTPEQTRLLERTYTRFHRAGAG
                     LDETAKNRMAEINERLAHLGTAFSHHLLGDEQDWFMELGEADCDGLPDSFVAAARAAA
                     QERGMAGKAIVTLSRSSVEPFLKSSTRRDLREKVFKAFTARGDNNNDNDNNAAIVEIL
                     SLREESAKLLGFSTFAAYRLEDSMAKTPEAVRGLLERVWKPARARALADRDALQALVA
                     EEGGNFALAPWDWRYYAEKLRQRRANFDDAAIKPYLVLDHMIDAAFDCATRLFGVTFS
                     ERKDIPVWHPDVRVWEVK"
     gene            1375..1635
                     /locus_tag="VGA15_20665"
     CDS             1375..1635
                     /locus_tag="VGA15_20665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590147.1"
                     /translation="MSSQPSPTSARQIVWPSVVTVISAAILIGAEVFGAAFAGGWALA
                     ILLGLDDTGAHILQAVLFAIGVFIMAAFIRAAQRVEPFTRRA"
BASE COUNT          369 a          659 c          658 g          397 t
ORIGIN      
        1 cttcacctcc cagacccgga cgtccggatg ccagaccgga atatccttgc gctcgctgaa
       61 cgtgacgccg aacaggcggg tggcgcagtc gaaggcggcg tcgatcatgt ggtcgagcac
      121 gagataaggc ttgatcgcgg catcgtcgaa attggcgcgg cgctgccgca gcttttcggc
      181 gtagtagcgc cagtcccacg gcgccagcgc gaagttgccg ccctcctcgg cgaccagcgc
      241 ctgcagggca tcgcgatcgg ccagcgcccg cgcccgcgcc ggtttccaga cccgctccag
      301 gaggccgcgc acggcctcgg gcgtcttggc catcgaatcc tcgaggcgat aggcggcgaa
      361 ggtggaaaag cccaacagct tcgcgctctc ctcgcgcaag ctcaggatct cgacaatggc
      421 ggcattattg tcgttgtcgt tgttgttgtc gccgcgcgcg gtgaaagcct tgaacacctt
      481 ttcgcgcaga tcgcgccggg tggagctttt gaggaacggc tcgaccgatg accgcgacag
      541 cgtcacgatc gccttgccgg ccatgccgcg ctcttgggca gcggcccttg ccgccgcgac
      601 aaagctgtcc ggaaggccgt cgcagtcggc ctcgccgagt tccatgaacc agtcctgctc
      661 atcgccgagc agatggtggc tgaacgccgt gccgagatgc gccagccgct cgttgatctc
      721 ggccatccgg ttcttggcgg tttcgtcgag cccggcgccg gcgcggtgga agcgcgtata
      781 ggtccgctcc aagagccgcg tctgctcggg cgtgagcccg agcgtggcct ggttgccgtg
      841 cagcagcgcg atccggccga acagcaccgc attcatcatg atcgggttcc agtgccgcgc
      901 catccgcagc gacacttcct tgtcgatctc gagcagcgtc gggttggaat gcgccgacac
      961 caggtcataa aacaccgccg acaccttgga gagcagtttt ccggcgcgct ccagcgccgt
     1021 gatggtgttg gcgaagtcgg gcacggaggg atcgtaggtg atcgcggtga tctcggcggt
     1081 gtgatcggcg aacgcctgtt cgaacgcagg caaaaaatgt tccggcgcaa tctcggcgaa
     1141 tggcggcgtc tcgaacggtg tctgccacgc catcagcagc gggttcggag tggcttgcga
     1201 gggaaccaat gtttccaagg tttctgacat gacaattccc gtttgcgtcc taaatgattg
     1261 aggaagttat atcacacgct ggggcctttt ttgggccttt tgcgcttgat agcgcgggcc
     1321 ttttcggaga gattgcggct ccctgacagg acctagccca aaggacttgg ccccatgagt
     1381 tcgcagcctt ccccgaccag cgcccgccag atcgtctggc ccagcgtcgt caccgtcatc
     1441 agtgcggcga tcctgatcgg cgccgaagtg ttcggcgcgg cgttcgccgg cggctgggcg
     1501 ctcgcgatcc tgctcggcct cgacgacacc ggcgcgcaca tcctgcaggc ggtgctgttt
     1561 gcgatcggcg tcttcatcat ggcggcgttc atccgcgccg cgcagcgcgt cgagccgttt
     1621 acgcgacgcg cgtaagtcgg ctgagcaaca aagccgcgcg caagctggcg tcgaacgcta
     1681 acgcctcgtt aaaggaactt aacatctgtt cacgttcagc gtcgctcacg cgcgcgccga
     1741 gtggcgctgc aagcactcgt tagggaaatt tccccacagc ctaaaaaaaa cgttgcgaag
     1801 cggactcaaa acccctattt ccactcttgc ccaatttcgc acgtgcctgt ggtcgtgtgt
     1861 cagtcggtag gtatccggac aagaggccgg acagccgcca agggacgaag aaccgagggt
     1921 cgctcaagtc aaacctaggc tggagcgtcc agcatctctc tcaagagatg ccgttgaagg
     1981 tctcttcgct ccttgcaacc gtgactggca gccggaggcg aaccggcgca cctcgttctc
     2041 aacgggggac gcgacttaaa gcaacgacgg atcgggcttt ttt
//
LOCUS       DASWGM010000742         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1308000,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000742 DASWGM010000000
VERSION     DASWGM010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308000"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VGA15_20670"
     CDS             <1..1435
                     /locus_tag="VGA15_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX9590148.1"
                     /translation="ITTPVNDAMFISGLGHVLSISGYHMAVVAGVVFFAVRALLALIP
                     ALTVGFPIKKWSAAAALAAAAFYLLLSGAEVATQRSFFMTAVVLIAVMVDRRAVTFRT
                     LAVAAMIVLALAPEALVHPSFQMSFAATLGLVALVQIGMPRLFASPDNSATARVALWG
                     GREIMTLALASFVAGLATTPYAAFHFHRVTPYGVLANLAAMPVVSAVVMPAGLLGLLA
                     MPFGLDGVFWQLMGWGIDWMIVVTEWVAALPGAIGRMAAFGIGPLIAASAGIVLLGLL
                     RTPLRWAGSAVLVLAVVWALAVPQPDILISGDGHNVGVRGRDGRLHLMRTAKDAFLVK
                     EWLAADADARQPGDASLADGVSCDEAGCVTQLADGGLVALALRSDALSDDCERAALVV
                     AARQAPPSCPSPVIGQDRLRRQGAMALRRTRDGFAVDAVKPKGIDRPWSPATAGDTEA
                     ELNLATRPAAPRAVDATPAEADQQTED"
     gene            1585..1782
                     /locus_tag="VGA15_20675"
     CDS             1585..1782
                     /locus_tag="VGA15_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590149.1"
                     /translation="MDPIKLRMRYLLGIFLAAVVTAAALRHVLHVQVGLAREDIRTGV
                     LITAGIGIFLMIAFFRWSRRL"
     gene            complement(1790..2143)
                     /locus_tag="VGA15_20680"
     CDS             complement(1790..2143)
                     /locus_tag="VGA15_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590150.1"
                     /translation="MAAIADVLSREIHAEIHATTSRQPIMNMDVIVSANKVDRSGRIV
                     KEHLILGTDKRDAEEQRDLWLSENPSIKVVKIHRVKREPQTLLTLIGSKHVPRVSIMV
                     EYEEPDAAEADVPAE"
     gene            complement(2410..2628)
                     /locus_tag="VGA15_20685"
     CDS             complement(2410..2628)
                     /locus_tag="VGA15_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590151.1"
                     /translation="MVDKVTWQQVGRVTEPGRYMFRFGWLTVTAEDLAVWEKLPNAAF
                     SLVRTATDAEEFRLGAFELRENPWLSEK"
BASE COUNT          501 a          941 c          996 g          647 t
ORIGIN      
        1 gatcacgacg ccggtcaacg atgccatgtt catctccggc ctcggtcacg tgctgtcgat
       61 ttccgggtac cacatggcgg tggtcgccgg cgtggtgttc ttcgcggtgc gcgcgctgct
      121 ggcgctgatc ccggcgttga cggtcggctt tccgatcaag aaatggtcgg ccgccgcggc
      181 ccttgccgcc gccgcgtttt atcttctgtt gtccggcgcc gaggtcgcca cgcaaagatc
      241 gttcttcatg acggcggtgg tgctgatcgc cgtgatggtc gatcgtcgcg ccgtcacctt
      301 ccgcacgctc gcggtggcgg cgatgatcgt gctggcgctc gcgccggaag cactggtgca
      361 tccgagcttc cagatgtcgt tcgcggcgac gcttggcctg gtggcgctgg tgcagatcgg
      421 catgccgcgt ttgtttgcct cgcccgacaa ttcggccacc gctcgcgtcg cgctatgggg
      481 cggacgggag atcatgacgc tggcgctggc ctccttcgtc gcgggactgg cgacgacgcc
      541 ctatgccgcc tttcactttc accgggtgac gccctatggc gtcctcgcca atctggcggc
      601 gatgcctgtg gtgtcggcgg tggtgatgcc ggccgggctg cttggcctgc tggcgatgcc
      661 gttcgggctc gacggcgtgt tctggcaact catgggatgg ggcatcgact ggatgatcgt
      721 ggtgacggag tgggtcgcag cactacccgg tgccatcggc cgcatggccg cattcgggat
      781 cggcccgctg atcgcggcga gcgcggggat cgttctgctc ggactattgc gcacgccgct
      841 gcgctgggca ggctcagccg tgctggtgct tgcggtggtc tgggcgctgg cggtgccgca
      901 gccggatatc ctgatttccg gcgatggcca caatgtcggc gtccgcggtc gggatgggcg
      961 gctgcatctg atgcggacgg ccaaggatgc gttcctggtg aaggaatggc tggctgccga
     1021 cgccgatgct cggcagcctg gggacgcctc gcttgcagac ggcgtctcat gcgacgaggc
     1081 tggttgcgtg acgcagctgg ccgatggcgg gttggtggca ctggcgttgc ggtcggacgc
     1141 cttgagcgac gattgcgagc gcgcggcgct ggtggtggcg gcgaggcaag cgccgccttc
     1201 atgtccgtcc ccggtgatcg ggcaggatcg tttgcgacga cagggtgcga tggcgctgcg
     1261 gcgaacccgt gacggatttg cggtcgatgc ggtcaagcca aaaggcatcg atcgtccgtg
     1321 gtcgccggcg acggcaggcg acacggaggc cgaactaaac ctcgcgacac gccccgcggc
     1381 gccgcgtgcc gttgacgcga cgccggcgga agccgaccag cagacggagg actgattgct
     1441 catttcggtc gcggcaaacc cggcgccggt tcgtgccatc ggcgtgtctg ggcattgccg
     1501 caaattgcaa cgggggcaga gcaaaaggcg ttcaacctga ggggtgtctt gaaaggtaac
     1561 ctgtgccgat gttaaatcta aacgatggac ccgatcaagc ttcggatgag atacctgctc
     1621 ggtattttcc ttgcggcggt ggtgaccgcc gccgcgttac gccatgtcct tcacgttcaa
     1681 gtcggcctcg cccgcgaaga tattcgcacc ggggttttga taaccgctgg gatcggcata
     1741 tttctcatga tcgcgttctt ccgatggagc aggcgtctgt gaacggcttt cattccgcgg
     1801 gaacgtcggc ctctgcggcg tcgggctcct cgtactccac catgatcgaa acgcgcggga
     1861 catgcttgct gccgatcagc gtcagcaagg tctgcggctc tcgcttcacg cggtgaatct
     1921 tcacgacttt gatcgatgga ttttctgaca gccagaggtc tctttgttct tcagcatccc
     1981 gcttgtcggt gcccaaaatc aaatgctctt tcacgatcct gcccgaacga tcgaccttat
     2041 tggctgacac gataacgtcc atattcatta ttggttgtcg cgatgtcgtt gcatgtattt
     2101 cggcatgtat ttcgcgcgat agaacatctg cgatagcagc catttgcgac ctccaaagct
     2161 cgaccccccc tgcatcaggc ccagttgagc gttggtaacc gagtccgcgc gcggcaaatg
     2221 tgaactgggt cacttaccgg acaaatctcg gccgaccgga attgcacatg aacgatgaga
     2281 agcgacgacg cccattcatg ccggctggct tcgctggcat gtgtctctcg gcaggagcga
     2341 cgcgggtcga aagccggcct gcaggaggag gatggacgcg gtgttgaacc gatatttccc
     2401 gaaaatgctt cacttctcac tgagccaggg attttcccgc agttcgaagg cgcccagccg
     2461 aaactcctca gcgtccgttg ccgtcctgac cagcgaaaac gccgcattcg ggagcttctc
     2521 ccagaccgcc agatcttccg ccgtaaccgt gagccacccg aaccggaaca tgtaccggcc
     2581 cggctcggtc actcggccca cttgttgcca agtgacttta tcgaccaatc atctttttcc
     2641 ctgtgaagtc tgcaaccgac tcgggcggcg gaacggggat tcgaggcgtc atgccggcct
     2701 tgaacggtcc ttcacgcgtc cgcatttcga aggcgtcgag ctgcaacgcg aacgtcgcca
     2761 gcgccagcct cagattattg aactcttcgg ctcgcgcgtc gtcgcgatga cccgtagcgc
     2821 tgtcgcgtgc ggttcgatgc gggtcaatat tcattgacgc tgctcgcgat cgccccgccg
     2881 ggcgcaaccg gcgggggtcc aaacggatgt ttcaatcaac ggagacgcgc ggcgttgcgt
     2941 tggcagcgga ctgcgcatgc tccgtaaccc gatcaaccac cggcaattgc aagaccgtca
     3001 cgcgctgccc ttcgcagagc tgcgtcacca gcacatggct tccatctgga aattcgatcg
     3061 catcatggtg acgatgcgga acatc
//
LOCUS       DASWGM010000743         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1766988,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000743 DASWGM010000000
VERSION     DASWGM010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766988"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..94)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            complement(153..857)
                     /locus_tag="VGA15_20690"
     CDS             complement(153..857)
                     /locus_tag="VGA15_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX9590152.1"
                     /translation="MTLPVIHRVTQLDLSVQPWSWRFAEERRADIDAHFAIQQREKPG
                     IWNGRILLGRDPDFTEGGFSAHYFEADFASFLAWRDWGYPDKDVFNGFGMGALRCSDG
                     AFVLGEMGQHTANAGRIYFPSGTPDLDDVSAGALDMSGSIAREVEEETGLTPADYRAG
                     AHWDCVVTGAAIAMIRILDVDSSGEALQARIEANLARQQQPELSAIHLVRDVGDLTAA
                     MPRFVTAFIEAQSASP"
     gene            complement(876..1904)
                     /locus_tag="VGA15_20695"
     CDS             complement(876..1904)
                     /locus_tag="VGA15_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9590153.1"
                     /translation="MRTMFGRLAGALLALFLMSGLAAAQSKITIAVGGGACLCYLPTV
                     LAKQLGEFDKAGLAVELVDLKGGSDALKAVLGGSADVVSGYFDHCVNLAAKKQELQAF
                     VVYDRYPGLVLVVSPSHTSEIKSIKDLAGKKVGVSAPGSSTDFFLKYLLKKNGLDPAS
                     ASVIGVGLGATAVAAMEQGQIDAAVMLDPSVTVLQGSHPDLRILSDTRTQKDTLDVFG
                     GEYPGGALYSTTAWVSSHEKDVQALTDAIVNTLAWIHAHSAEDIMAKMPAETVGKNKE
                     LYLAALKNTIPMYSETGKMDPRGADAVLAVFSESSPEVAKANIDVSKTWTNKYVEQAG
                     KTTGMNAK"
BASE COUNT          425 a          694 c          655 g          438 t
ORIGIN      
        1 gttgccggat cggccgcatc tcccctgctc ggggacgcca ccttgctcgg gagcaggttg
       61 gcgttgagcg tcagctcaac tcttgatgtt gcgaacgcca tagacggaaa aaaccagtat
      121 ggcaaggcgc ctcgcaccga cggcaccgtg gctcagggtg acgcagactg cgcttcgata
      181 aacgcggtga caaagcgcgg catggccgcg gtgagatcgc ccacatcgcg cacgaggtgg
      241 atggcggaaa gttcgggctg ttgttgccgg gcgagattgg cttcgatcct ggcctgcagc
      301 gcctcgcctg aactatcgac gtccaatatc ctgatcatcg caatcgcagc accggtaacc
      361 acgcaatccc agtgtgcgcc ggcccgatag tcggccggcg tcaaaccggt ttcctcctcg
      421 acctcgcggg cgatgctgcc ggacatatcg agcgcgccag cgctgacatc gtcgagatcg
      481 ggcgttcccg acggaaagta gatgcgcccg gcattcgcgg tgtgctggcc catttcgccc
      541 agcacgaacg cgccgtcgga gcagcgcagc gcgcccatcc caaaaccgtt gaacacgtcc
      601 ttgtccggat aaccccaatc gcgccaggcc aggaagcttg cgaaatcggc ctcgaaatag
      661 tgggcgctga aaccgccctc ggtgaagtcg ggatcacgcc ccagcaggat gcgtccattc
      721 cagattcccg gtttttcgcg ctgctggatc gcaaaatgcg cgtcgatatc ggcgcgccgc
      781 tcttctgcga agcgccacga ccatggctgc acactcagat caagctgcgt gacgcgatga
      841 atgaccggaa gcgtcatgca aatttgcttg ccaatctatt tcgcattcat ccccgtcgtc
      901 ttgccggcct gttcgacata tttgttggtc caggtcttgc tgacgtcgat attcgctttc
      961 gcaacttcgg gcgagctctc gctgaacacc gcgagcacgg catcggcgcc cctgggatcc
     1021 atcttgccgg tttccgaata catcgggatc gtgtttttca gcgcggcgag ataaagctcc
     1081 ttgttcttgc cgacggtttc cgccggcatt ttcgccatga tatcttcagc cgaatgggcg
     1141 tggatccagg cgagcgtatt cacgatcgca tcggtcaggg cctgcacgtc cttctcgtgc
     1201 gaggacaccc acgccgtggt agagtagagc gcgccgccgg gatattcgcc gccgaaaaca
     1261 tccagcgtat ccttttgggt gcgggtatcg ctgaggatgc gcagatccgg atggctgcct
     1321 tgcagcacgg tgaccgacgg atccagcatg acagccgcgt cgatctggcc ctgctccatc
     1381 gccgcgaccg cggtggcgcc gagaccgacg ccgatcacgg aggcgctcgc cggatcgagg
     1441 ccgtttttct tcagcaaata tttgagaaag aaatcggtcg aggaacccgg cgcgctgacg
     1501 ccgactttct tgccggccag atccttgatc gatttgattt cgctagtgtg cgagggcgac
     1561 accaccaata cgagaccggg gtagcggtca taaacgacaa aagcctgcag ctcctgcttc
     1621 ttggcggcga gattgacgca gtggtcgaaa tagccggaga ccacgtcggc gctgccgcca
     1681 agcacggcct tgagcgcgtc ggagccgcct ttcagatcga ccagttcgac ggcaagcccc
     1741 gccttgtcga attcgccgag ttgcttggcc agcacggtcg gcagatagca caggcaggcg
     1801 ccgcccccga ccgcgatggt gatcttgctt tgggctgcgg ccagaccaga catcagaaac
     1861 agcgctaaaa gcgccccggc caatctacca aacatcgttc tcatgatttc ctccgcggcc
     1921 cttgttccgt caggcgcacg gtagaacagc gcgggcctct tgagaaggtc gcatccagat
     1981 cagactctgg ttacgaaaac tgcttcaaac atgaattcgc gatctcgcgg cgggatgcgc
     2041 ccgaggtttg catttatctt ccgcccccct tggagggcgt ggggaatgcc gggtgcccgc
     2101 tgcacccgca gcctcgtgtg cgtagtgtag aaagcacacg agtcttcacc acggtcgcac
     2161 cggaacaccc ggcattcccg cacgcaatgg ttttaacggc ttatgtcgtg ct
//
LOCUS       DASWGM010000744         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2168087,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000744 DASWGM010000000
VERSION     DASWGM010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2168087"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VGA15_20700"
     CDS             <1..666
                     /locus_tag="VGA15_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX9590154.1"
                     /translation="EIAALKAKLRRIPFIDPIDIRYRRFETMPKPVAQAVMFCLMDVS
                     GSMSEHMKDLAKRFYMLLYVFLTRRYRHVEIVFIRHTDRAEEVDEDTFFHGPASGGTL
                     VSSALQAMHEIVRSRFRPADWNIYAAQASDGDNSGSDGAAVSRLLTEMILPVSQFFAY
                     LEVGETGGSTFDMPDSSLWTLYQRLRAEGVPLSMRKVSDRSEIFPVFHDLFHRRRKQE
                     KAP"
     gene            663..2207
                     /locus_tag="VGA15_20705"
     CDS             663..2207
                     /locus_tag="VGA15_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX9590155.1"
                     /translation="MTATAGCLFEGADWDFTKLQRIHDACEEIARSELGLDTYPNQIE
                     VITAEQMLDAYSSAGMPLFYKHWSFGKHFAHHEAFYRKGIMGLAYEIVINSSPCISYL
                     MEENTATMQTLVIAHAAFGHNHFFKNNYLFRQWTDAEGILDYLDFAKSYVAQCEERHG
                     RLAVEQTLDAAHALMSHGIDRYPGKKKLDLRAEEKRAGERRQHEESAFNDLWRTVPTG
                     PAKSAELLSSERRRNLLGLPQENLLYFLEKTAPRLQLWQRELLRIVRHIAQYFYPQSQ
                     TKVMNEGTATYVHYRIMTRLHEQGRLSDGNFLEFLSSHTNVVFQPDFDDPRFSGFNPY
                     ALGFAMMQDIERIVSNPDDEDRQWFPDIAGTGDVMAVLRNVWANYRDESFISQFLSPR
                     LIRHLRLFHLHDDPEQRAGIRVDAIHDERGYRRVRRELARQYDVGYIDPNIEVVDVDL
                     AGNRRLMLRHAVVKGAQLNEVDAKRVLQHLADLWSYDVSLVEVDASDNVLKEYVLNPR
                     ALAAAA"
     gene            complement(2319..>2874)
                     /locus_tag="VGA15_20710"
     CDS             complement(2319..>2874)
                     /locus_tag="VGA15_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9590156.1"
                     /translation="WLFLSWIPSFFYQNYQQDLTRSAFYSAGVFLAGVIGDTLGGVVS
                     DRILHRTGDRAAARRNVIVAGMLGGFLFLIPVILVHDVNVAAISLAAAFFSVELVVAP
                     IWAVPMDIAPRYSGSASGMMNFGFGVAGIISPFVFGYLIDRTGSWTWPFAGSIGLLLL
                     GAGLAFHMHPDRPFEAAPKGARND"
BASE COUNT          608 a          866 c          842 g          558 t
ORIGIN      
        1 gagatcgccg cgctaaaggc aaaactgcgc cggattccgt tcatcgatcc gatcgacatc
       61 cgataccgcc gcttcgaaac catgccgaag ccggtagccc aggccgtcat gttttgtctg
      121 atggacgtgt ccggctcgat gtccgagcat atgaaggatc tggccaagcg gttctacatg
      181 ctgctctacg tatttctgac gcggcgctat cgccacgtcg agatcgtctt cattcgccat
      241 accgaccgcg cggaagaggt tgacgaggat actttctttc acgggccggc atcgggcggt
      301 accttggtat cgagtgcgct gcaggcgatg cacgagatcg tcaggtcacg gtttcgcccc
      361 gcggactgga acatctacgc cgcacaggcc tctgacggag acaattccgg ttcggacgga
      421 gctgccgtga gccggctgct taccgaaatg atactgccgg tcagccagtt cttcgcctac
      481 ctggaagtcg gcgagaccgg cggcagcacg ttcgatatgc ccgattcatc gctctggacg
      541 ctgtatcagc gcttgcgcgc cgaaggggtg ccgctgtcga tgcgcaaggt cagcgaccgc
      601 agtgaaatct ttccggtctt tcacgacctg tttcaccgtc gtcgcaagca ggagaaggcg
      661 ccatgaccgc gactgccgga tgcctgttcg agggagcgga ctgggacttc acgaagttgc
      721 agcgcatcca cgacgcctgc gaggagattg cgcgatctga actcgggctc gacacctatc
      781 ccaaccagat cgaggtcatc accgccgagc agatgctgga tgcatactcc tcggccggca
      841 tgccgctgtt ctacaagcac tggtctttcg gcaagcattt cgcacatcac gaggcgttct
      901 atcgcaaagg cattatgggc ctcgcctatg agatcgtgat caacagttcg ccgtgtattt
      961 cctatttgat ggaggaaaat accgcgacca tgcagacgct ggtgatcgcc cacgcggcgt
     1021 tcggccacaa ccatttcttc aagaacaact atctgttcag gcaatggacc gacgccgagg
     1081 gaattctcga ttatcttgat ttcgccaaaa gctacgtggc gcagtgcgag gagcggcacg
     1141 gccggctcgc ggtcgagcag acgctcgatg cggcgcacgc cctgatgtcg catgggatcg
     1201 atcgctatcc cggcaagaaa aaactcgatc ttcgcgcgga agaaaagcgc gccggagagc
     1261 gtcgccagca cgaggagagt gcattcaacg atctgtggcg gaccgttccg actggccccg
     1321 ccaagagcgc ggaattgctg agttcggagc ggcgccgcaa cctgctcggg ctgccccagg
     1381 aaaatctgct gtatttcctc gaaaagaccg cgccgcgact gcagctatgg cagcgcgagt
     1441 tgctccggat cgtgcggcac atcgcgcagt atttctatcc ccagagtcag accaaggtca
     1501 tgaacgaggg caccgcgacc tacgtgcatt accgcatcat gacccggctg cacgagcagg
     1561 gccggctttc cgacggcaat tttctggaat tcctgtcgtc gcacaccaac gtggtgtttc
     1621 agcctgactt cgacgatccg cgcttttcgg gttttaatcc ttacgcgctc ggcttcgcga
     1681 tgatgcagga catcgagcgc atcgtctcca atcctgacga cgaggatcgt caatggttcc
     1741 cggacatcgc cggcaccggt gacgtgatgg cggtcctgcg taatgtctgg gccaactacc
     1801 gcgacgaaag tttcatcagt caattcctca gcccgcggct aattcggcat ttgcggctgt
     1861 ttcatctgca tgacgatccg gagcaacgcg ccggcatcag ggtcgatgcc atccatgacg
     1921 aacgcggtta tcgtcgtgtt cgtcgtgaac tggcgcggca gtacgacgtc gggtacatcg
     1981 atccgaacat cgaagtggtc gacgtcgacc ttgccggaaa tcgtcgcctg atgttgcgcc
     2041 atgcggtcgt gaaaggcgcc cagttgaacg aggtggacgc caaacgcgtg cttcagcacc
     2101 tcgccgatct ctggagctat gacgtatcgc tcgtcgaagt cgacgcgtcc gacaacgtcc
     2161 tcaaggaata cgtcctgaac ccccgagcgc tcgccgcagc agcgtaaatg cttgccggtg
     2221 aatttcggac ggctttgatg gccgcgccgc gcgaggtgct gcgttttccg aatccgccag
     2281 gtcaatcgat acttaatcgc ggcaaacgag gcgaacgttc aatcgttcct ggcgccttta
     2341 ggtgccgcct cgaacggccg atccggatgc atgtgaaacg caagtccggc gccgagcaac
     2401 aatagaccaa tcgagccggc aaatggccag gtccaacttc cggtccgatc gatcagatag
     2461 ccgaacacga agggcgaaat aatgccggca acgccgaagc cgaaattcat catgccgctc
     2521 gcggatccgg aatatcttgg cgcaatgtcc atcggaactg cccagatcgg cgcgaccacc
     2581 agctcgacgg aaaagaatgc cgcggcgagt gagatcgccg caacgttgac atcgtgaacc
     2641 agtatcactg gaatcaggaa cagaaagccg ccgagcatac cggcgacgat gacgttacgt
     2701 cgcgccgcag cccggtcgcc ggtgcggtgc aggatccggt cgctcaccac gccgcccagg
     2761 gtgtcgccga tcacgccggc aaggaagacg ccggctgaat agaatgccga tcgcgtcagg
     2821 tcctgctgat agttctggta aaagaacgac ggtatccagc tcaggaacag ccac
//
LOCUS       DASWGM010000745         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_623000,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000745 DASWGM010000000
VERSION     DASWGM010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623000"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..1482
                     /locus_tag="VGA15_20715"
                     /pseudo
     CDS             373..1482
                     /locus_tag="VGA15_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1644..>1942)
                     /locus_tag="VGA15_20720"
     CDS             complement(1644..>1942)
                     /locus_tag="VGA15_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9590157.1"
                     /translation="VEEVVRWATVNGARVLGFDDIGVLAPGKLADIAIFNLSNPRYFG
                     LHDPLTGPVAGAGAADLRILMVGGRIVVENGAVPGLDLAKLRHDAAGIISRIAN"
BASE COUNT          396 a          592 c          570 g          384 t
ORIGIN      
        1 caacccagcg caaccacatc ccgcccgaaa ggatcgcctg cccaaacatg caggcaacca
       61 ccagcggcac cgccacaatt ctggcatagg tcaggagatt agggaccgcc aagggccgtc
      121 ccgggagggg ccgcgtggtg actgcatcca tttttcaaac ataatcgttt tacgctgctg
      181 tgcacactgg tcggtagcat taatattcgg ctatgggccg ctgaccacgc tggcgcatct
      241 tcgatttagt gtcattgccc ggcttgaccc acggctgtcc ggcacggttt gtgcttgaag
      301 gagtgcacgg cattgattct actcgattcc agttggttgc gaatcatctg gacaggagaa
      361 aggatcaacg ccgtgccgca ccagaatatc gtttttcatg acgttctgaa gcatattcct
      421 tggttgatcg tcgatcggct tgtgaagcag tacgatgccg actgggatga tcgctgcatc
      481 aagaccaggg ctcatctgat cgcgatgctg tacgcacagt tttccggtgc gcggggcctg
      541 cgcgagatcg aagcgaacct gaaaagccat gccagcaagc tttatcatct gggcggcagc
      601 accgtatcga agtcggcgct gtcgaccgcc aatgctgagc gtccggttga agtctttggt
      661 ggcttgcttt cggcgctgat ggcttcgttg caggccggct accggcgcaa aatcggcgat
      721 tgcgtgcgtc tgattgattc gaccagcgtg cagctgagca gcctgagcgg caaatgggca
      781 acgttttcgg cgggggtttg cggcgctaag gcgcatatca tctacgatcc cgatgccgac
      841 caaccgctct atctcatggt gacgccttcc aacgtcaatg acatcacggc cgccaaggag
      901 atgccgatcg aggccggagc gacctacgtc ttcgacctcg gctattacga ttatggttgg
      961 tgggccacgc ttgaccaggc gggctgccgc atcgtcaccc gcctcaagtc caatacgcct
     1021 tttacggttg tcgatgatct gccggtcccg ccgggatcgt cgatcctgag cgaccgcacc
     1081 ggatatcttc ccaatcggct cgcggcatcc cgcaagaacc caatgtcggg gctcgtgcgc
     1141 gaagttcaag tcatgatcga gaccggcaag gtgctgcgta tcttcaccaa tgatctggag
     1201 gccagcgcgc aggaaatcgc cgacctgtac aaacgtcgat gggccatcga gttgttcttc
     1261 cgatgggtca aacagacctt gaagatcagc cacttcttgg ggacctccga aaatgccgtg
     1321 cgcattcaaa tcgcggtggc gctgatcgct ttcttgttgc tgcgtctggc ccatgacgcc
     1381 aacaaaatcg ttgcaagccc gctcgccttt gcgcggctca ttcgagccaa cctgatgcac
     1441 cggcggccaa tcgcagaact cctcaaacct cagccaacgc ccgagccgca acagcccaga
     1501 tttgactttg ggccgtacgc gacccgcgcg gcgcaacgcc gccggaacac acgcgcgtcc
     1561 gtcgccatgg agaaagcagc atgataagca caaaccgtgc cggacagccg tgcgacaagc
     1621 gcgggcatga cggcggagag gatctaattc gcgatccggc tgatgatccc ggcggcgtca
     1681 tgccgtagct tcgcaaggtc aaggcccgga acggcgccgt tctcgaccac gatgcggcct
     1741 cccaccatga gaatcctgag gtcggcggcg ccggcgccag ccaccgggcc ggtcaggggg
     1801 tcgtgcagtc cgaaatagcg cgggttcgac agattgaaga tcgcgatgtc ggcgagcttg
     1861 ccaggagcga gtacgccgat gtcgtcgaaa ccaagcacgc gggcgccgtt cacggttgcc
     1921 caccgcacca cttcctcgac tg
//
LOCUS       DASWGM010000746        10622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1037235,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000746 DASWGM010000000
VERSION     DASWGM010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037235"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..867)
                     /locus_tag="VGA15_20725"
     CDS             complement(139..867)
                     /locus_tag="VGA15_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009032188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9590158.1"
                     /translation="MRAHQIMTRPVITATPETTIVEAANTMLQRHVSGLPVTDAAGKL
                     VGIISEGDFIRRSEIGTQRKRGRFLKFILGPGKAATDFVHEHGRKVAEIMTPEPLTIT
                     EDTALEEIVELMEKNNVKRLPVMRGDRIVGIVSRANLLQAVASLAREVPDPTADDDHI
                     RNRVIDEIEKNDWCPFGLDVIVRGGIVHLSGVITEERSRKASVVAAENITGVTKVHDH
                     LCWVDTMSGMYLNSPEDDNLAKAS"
     gene            1042..1410
                     /locus_tag="VGA15_20730"
     CDS             1042..1410
                     /locus_tag="VGA15_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590159.1"
                     /translation="MTELSEQSAALVEQSVADTATRLTKVNKNALDFLMGAQKVMLEE
                     LVFAGNEMLERTRTETHLLSEFVSKMAGSHSVKDLKTMSQECGQHQIDFIRRDSERLF
                     KHGERMIEATAKLLGNRPES"
     gene            1653..3338
                     /locus_tag="VGA15_20735"
     CDS             1653..3338
                     /locus_tag="VGA15_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9590160.1"
                     /translation="MLARLTGGISPVALSLAYIDWASHLASAPQRQMEISQDALRSVR
                     QFFESALRYFSPGQGPWSLIKPQPQDRRFAKPEWENPPFNLLAQAFLLKEQWWHDATT
                     GVRGVAPQNEAIVEFSVRQVLDMLAPSNFAATNPEVLQKAFRSGGENFIFGWQNWCSD
                     WMHLLSLSAGKSNENQDFVVGKTVATSPGKVVFRNELIELIQYQPTTGKVHPEPILIV
                     PAWIMKYYILDLSPRNSLVKFLTDQGFTVFMISWRNPGAADRDIAFDDYRKLGVEAAL
                     AMIGVIVPGPQIHALGYCLGGTLLSIAAAAMARDDIDRLRSITLLAAQTDFTEVGELT
                     LFINESQVAFLEDMMWQRGVLDTTQMAGAFQLLRSNDLIWSKITRDYLMGERAPPSDL
                     MAWNADATRLPYRMHSEYLRRLFLDNDLAEGRYLVEGKPIALSDIHTPMFVVGTARDH
                     VAPWKSTYKIHYQVDADVTYLLTSGGHNTGVVAPPNEPGHSYQVKTKPEDARYIGPDE
                     WLKVAPRIEGSWWPEWTKWLTAQSGEPCEPPRMGVEHAGGQPLPDAPGDYVRQ"
     gene            complement(3358..4197)
                     /locus_tag="VGA15_20740"
     CDS             complement(3358..4197)
                     /locus_tag="VGA15_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX9590161.1"
                     /translation="MMACDTAFRIAARRHLGDLTANHAATSEGDPVALHQMRIALTRL
                     RTAIWFFSPMVIDTEQPRIKGELKWLNTQLGVVRDLDVATEQMQAVDERRPQAIPDHR
                     AWAVKHANGHRHLAQALRSARYRRLIKIAFDWIENGPWSIRKGKRATKQRACSIATYS
                     AGKLARWRQKLLKKSRKLPEMDAKTRHRLRLLNKKLYYSIEFLEHLFPGKIFSRQRAA
                     LKYLRKAQKSLGQLNDDANSQSLAAALQRDGVGEPLQFLGPKREKRLVRKAAAAYRKL
                     AAL"
     gene            complement(4313..5833)
                     /locus_tag="VGA15_20745"
     CDS             complement(4313..5833)
                     /locus_tag="VGA15_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9590162.1"
                     /translation="MQESVFAFLTDPTMHPQVHRIDTHAASVFLEGDRALKIKRAVRF
                     PFLDYSTLEKRKAACDEEININRQFAPQIYRRVVPITQDSDGSLDIDGDGTPVEFAVE
                     MTRFDECQTIDHLAEAGEPDSDLVNAIADAIGASHAVAPRAAAEPWVESIPTFIEGNT
                     TAFRTAACFSERNIDDLNAASQSAYLRIRELLRQRGRDGYVRRCHGDLHLANIVLIER
                     KPVLFDAIEFDPTIASVDLLYDLAFPIMDFMRYNRHAAANALLDRYLAMTPLENLDAL
                     AALPLFVSLRAAIRANVVLARLGHACDDKADVMQSARAYFELAHLTIRPSAPTLVAVG
                     GLSGTGKSVLARALAPDVTPLPGAVVLRGDVLRKERFQVTETDRLPESAYRQEITAQI
                     YTVLVQRAVRILVQGQSVIVDAVFAHETERAAICDAARKLNVRFVGLFLATDLATRVR
                     RVGRRERDASDATPEIAEIQEKYNIGRVDWAVIDASGTPEQTLVQCQARIAHRDPA"
     gene            complement(5876..6859)
                     /locus_tag="VGA15_20750"
     CDS             complement(5876..6859)
                     /locus_tag="VGA15_20750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9590163.1"
                     /translation="MRAMVLLAPGEPLQMQERPDPSPDDGQIRVKVSACGVCRTDLHV
                     VDAELPGIKYPVIPGHEIVGRVDLVGSHVTTHGIGDRVGIPWLGYTCGVCRFCKDGME
                     NLCDRPLFTGYTRDGGFATHTIADARYAFPLGEDGDDVSIAPLLCAGLIGWRSLVMAD
                     NAERLGIYGFGAAGHIVAQIARWQGRSVYAFTRSGDVAAQDFARRLGAVWAGASGELP
                     DAPLDAAIIYAPAGELVPTALRAVRKGGKVVCAGIHMSDIPSFPYNLLWEERQLLSVA
                     NLTRQDGLDFLKIAPQAGIRTQTTAFPLAEANDVLKKLRTGRVLGAAVLKP"
     gene            7047..7889
                     /locus_tag="VGA15_20755"
     CDS             7047..7889
                     /locus_tag="VGA15_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX9590164.1"
                     /translation="MPIKDILLPLVGEPSAAAIAAIDKCVAVAGDIGARVTAMAVEED
                     ILVRPKVTISDDLDNTAAVEAVRSVSDARGLLKAFDAAAIRFAVRNEQKLSRLAAADI
                     AMNMAVCARLKDLSIFPVKPNDDRSEKLVERLIFESGRPILMCPEEFANELAVVFDKV
                     VIAWDHTAPAARAVADALPMLQAAANVRIITATDKITPAKQESGTALVSHLAEHGIKA
                     TFEAVKIEGSSVGKVFEAYVKANAIDLLIMGAYRHSRLNEIVSGGATKTVIGRPPCWV
                     MMSR"
     gene            8049..>10622
                     /locus_tag="VGA15_20760"
     CDS             8049..>10622
                     /locus_tag="VGA15_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEX9590165.1"
                     /translation="MSTYRLKNLLSPRSLALVGASPRQGSVGRAILANIHQAKFKGEF
                     GLVNSHYAEIDGIAAVSSLGKLPFVPELVVITAPAAAIPDLIDGAGQRGAAGAVIITS
                     GLGHGAGSLAESAERAAHKYGMRLIGPNCLGIMMPGANLNASFAAHMPCAGNLALISQ
                     SGAIATGMVEWAAQRAVGFSGIVSIGDQLDVDIADLLDYFAMDGKTRAILLYVEAIKD
                     ARKFMSAARAAARIKPVVVVKSGRMAQGAKAAATHTGALAGSDAVYDAAFRRAGILRV
                     SDLRELFDCAETLGRVYSPPGKRLAILTNGGGIGVLAIDRLAELGGTPAAITPATREK
                     LDAVLPPTWSGSNPVDIVGDADPARYAAALEVLLADASNDAILVMNVQTAIAPAGDIA
                     TTVTGLVSKYRQQHRDFAKPVLAVWVGADQKIIDLLSGAGIPNYPTEDDAVRGFMYLV
                     RHREVVAELAQVPPAMPSEFAPDADAARAIVAAALADGRQWLDPVEIKRLLEAYEIAM
                     VPTFAATDAEQAVAHASALFAQGATVVLKIMSRDIVHKSDVGGVVLNLTSTDAVRAAT
                     ADILARAKALRPEARISGVIVQAMMVRPKARELILGLADDPTFGTVIVFGRGGTAVEI
                     INDKALALPPLDLQLARDLIERTRVSRLLRAYRDVPAVEPDAVAKVLVKLAQMAADIP
                     EIRELDINPLLADQAGVLAVDARVAIGQAERKFGGSGPANFAVRPYPSQWQRHLEVKD
                     GWRVFVRPIRPEDEPLIHELLRHVTSQDLRLRFFASMKEFSHEFIARLTQLDYARAMA
                     FVALDEATNEVVGVVRIHSDSIYENGEYAILLRSDLKGRGLGWALMQLIIEYAKSEGL
                     KV"
BASE COUNT         2016 a         3163 c         3360 g         2083 t
ORIGIN      
        1 gccgaccggt gctcttccaa cgctgaaatt ggctccgttg gagaggtaag ttggcgcggc
       61 tcgcctggac actcctgtcc ggttggaggg cgtacatctc catcatttgg gatgcacgcc
      121 ctctgtcgcc cccgccgatc agctcgcctt tgccagattg tcgtcttccg gggagttcag
      181 gtacattccc gacatggtgt cgacccaaca caggtggtcg tggaccttgg tcacgccggt
      241 aatattttcc gcggcgacga ccgaagcctt ccgcgaacgc tcctccgtga tgacgccgct
      301 cagatggacg atgccgcccc gtacgataac gtcaagaccg aacgggcacc agtcgttctt
      361 ctcgatctca tcgatgacgc ggttgcggat gtggtcatcg tcggcggtcg gatcgggaac
      421 ctcgcgggca aggctcgcta ccgcctgcaa caggttggcg cgcgacacga tgccgacgat
      481 cctgtctccg cgcatcaccg gcaggcgctt gacgttgttc ttttccatca gttccacgat
      541 ctcctccagc gccgtgtctt cggtgatcgt gagcggctcc ggcgtcatga tctcggcgac
      601 tttgcggcca tgctcgtgaa cgaaatcggt tgccgccttg cctgggccga gaatgaattt
      661 cagaaaccgg ccgcgcttgc gctgggtgcc gatttcgctg cggcggatga aatcgccctc
      721 cgagatgatg ccgacaagtt tgccggcggc gtcggtcact ggcagcccgc tgacatgcct
      781 ctgcagcatg gtgttcgcgg cttcgacgat cgtggtttca ggggtggcgg tgatgacggg
      841 ccgcgtcatg atctgatgtg cgcgcattgg aggcctccgc gggttcgaga ttaacgccga
      901 tatttgacag cctattccaa ccggcgcgcc tcgatttgac gcaggtcaat gggcggccgg
      961 ggcgcgtaga cttgatgcag ctcaatgcga gctcgggggc gaaatcctat catttcaaaa
     1021 gaacatgaaa ggggagtcgt catgacagag ctttccgaac agagtgccgc gctcgtcgag
     1081 caatcggtgg ccgataccgc gacacgattg accaaggtga acaagaacgc cctggatttc
     1141 ctgatgggcg cgcaaaaggt catgcttgag gaactcgtat tcgccggcaa cgagatgctc
     1201 gagcggacac gaaccgaaac gcacctgttg tccgaattcg tctccaaaat ggccggatcg
     1261 cattcggtca aggacctgaa gacgatgtcc caggagtgcg gccagcatca gatcgatttt
     1321 atccgccgcg acagtgagcg cttgttcaag catggtgaac ggatgatcga agccaccgcg
     1381 aagctgttgg gcaaccggcc cgagagctga tccgtcttcg gaccgtcatc gattcgaagt
     1441 tcaacactcc gctcgatgtg aaggagatgg atcctgtgcc gccgatatcc agccagccaa
     1501 agccacaggc tgaagcaccg aggttgccgt gtcccgcgca tgccccgggc tacggcgaag
     1561 acggccgcgc cgtcgcctcg ggccgggctc ccgcgaagct cgcgcccgaa gcccagctgg
     1621 agaccgatca ggccgatcgc gcgtttcatg cgatgctggc gcggctgacc ggtggcatat
     1681 cgccggtcgc gctgtcgctc gcctatatcg actgggcctc gcatttggcg tcggcgccgc
     1741 agcggcaaat ggagatatcg caggacgcct tgcggagtgt gaggcagttt ttcgaatccg
     1801 cgctgcgcta tttttcgccc ggtcagggac cttggtcgct gatcaaaccg cagccgcagg
     1861 accggcgttt tgccaagccg gagtgggaaa atccaccgtt caatctgctc gcccaggcgt
     1921 ttcttctgaa agagcagtgg tggcacgacg ccaccaccgg tgtgcgcggc gtggcgccgc
     1981 agaacgaggc gatcgtcgag ttttcggtgc ggcaggtgct cgatatgctg gcgccgtcca
     2041 atttcgccgc gaccaatccc gaagtgttgc agaaggcatt ccggagcggc ggcgagaatt
     2101 tcatcttcgg ctggcagaac tggtgcagcg actggatgca cttgctgtcg ctatctgcgg
     2161 gcaagtcgaa cgaaaatcag gatttcgtcg tcggcaagac cgtcgcgacc tcgccgggca
     2221 aggttgtttt ccgcaacgag ctcatcgagc tgatccagta ccagccaacc actggcaagg
     2281 tgcatccgga gccgatcctg atcgtgccgg cctggatcat gaaatactac atcctcgacc
     2341 tgtcgccgcg gaattcgctg gtgaagttcc tgaccgatca aggctttacc gtattcatga
     2401 tctcctggcg caacccgggc gcagcggatc gcgatatcgc cttcgacgac tatcggaagc
     2461 tgggcgtcga agccgcgctc gccatgatcg gcgtcattgt gccgggccca caaattcatg
     2521 cgctcggcta ttgcctgggc gggaccttgc tgtcgatcgc cgccgcagcg atggcgcgag
     2581 acgatatcga cagattgagg tcgatcactc tgcttgcagc gcaaacagat tttaccgagg
     2641 tgggcgaact gaccttgttc atcaacgaaa gccaggtcgc gtttctcgaa gatatgatgt
     2701 ggcaacgcgg cgttctcgac accacgcaga tggcgggcgc attccagttg ctgcgctcga
     2761 acgatctgat ctggtcgaag attacccgcg actacctgat gggagaacgg gcgccgccca
     2821 gtgacctgat ggcctggaat gccgatgcca cgcgcttgcc ataccggatg cactccgaat
     2881 atctgcgcag gttgtttctc gacaacgatc tggccgaggg ccgttacctc gttgagggca
     2941 agccgattgc gctgtcggat attcacacgc cgatgttcgt ggtcgggacg gcccgcgatc
     3001 acgtggcgcc gtggaagtcc acctacaaga tacactatca ggtggacgcc gacgtgacct
     3061 acctcttgac cagcgggggc cacaatacag gcgtggtggc gccaccaaac gagccgggcc
     3121 actcctatca agtcaagacg aagccggagg atgcacgcta tatcggtccc gacgaatggc
     3181 tgaaggtagc accacggatc gaaggatcgt ggtggccgga atggaccaag tggctgaccg
     3241 cgcaatccgg cgagccctgc gagccgccgc gtatgggtgt tgaacacgcg ggcggtcagc
     3301 ctttgccgga tgcgccgggg gactatgttc gccagtagcg gttggcggcc cgttcactta
     3361 cagcgcagcc agcttgcggt atgccgcagc cgcttttcgt accagtcgct tctcgcgttt
     3421 cggaccgaga aattgcaagg gttccccgac gccgtctcgt tgcagagcgg ccgccaggga
     3481 ttggctgttg gcgtcgtcgt tcaactgccc gagagatttt tgcgctttac gcagatattt
     3541 cagcgcagcc cgttgccttg aaaatatttt gccgggaaac aagtgttcga ggaattcgat
     3601 tgaatagtac agcttcttgt ttagcagacg cagccggtgc cgtgtcttcg cgtccatttc
     3661 cggcaacttg cggctttttt tcagaagttt ttgccgccac cgcgccagct tcccggcgct
     3721 ataggtggcg atcgagcaag cgcgctgctt tgttgcccgt ttccccttcc tgatggacca
     3781 gggtccattc tcgatccaat cgaatgcgat tttgatcaga cgtcgatacc gggctgaccg
     3841 aagcgcctgc gccagatgtc gatggccgtt ggcgtgcttc acggcccatg cccgatgatc
     3901 gggtattgct tgcggtcgtc gttcgtcgac ggcctgcatc tgttcggtcg cgacatccag
     3961 atcgcgaacg acgccaagct gagtgttcag ccacttcagt tcgcccttta ttcgtggctg
     4021 ttcggtatcg ataaccatcg gcgagaaaaa ccagatagcc gtgcgcaacc gcgtgagcgc
     4081 gatgcgcatt tgatgcagag ctaccgggtc accctcgctt gtcgccgcat gatttgcggt
     4141 caaatccccg agatggcggc gcgccgcgat ccggaatgcg gtgtcgcagg ccatcattgg
     4201 attcaaccgc gaaacggcgc ggcggcgtgg ccgctgcgtc ggttcgatgc ggatcgcgcc
     4261 tttgcgcttt gatttcgggg atggcggcat tgccgtttcg cgcgatcggc tctcaggccg
     4321 gatcacggtg agcgatccgg gcttggcact gtaccagggt ttgctctgga gttccggatg
     4381 catcgatgac agcccagtca accctaccga tattatattt ctcctgaatt tcggcaattt
     4441 ccggcgtcgc gtcggatgca tcgcgctcac ggcggccgac gcggcgcact ctggtcgcaa
     4501 ggtcagtcgc gagaaagagc ccgacaaacc ggacattcag cttgcgcgcg gcatcgcaga
     4561 ttgcggctcg ttccgtttca tgcgcgaaca ccgcgtcgac gataacggat tgaccttgga
     4621 caagaatccg gaccgcgcgc tgcaccagga ccgtgtagat ttgcgcggta atctcctgcc
     4681 ggtatgcgct ctcgggcagc ctgtccgtct cggtgacctg aaatcgttcc tttcgcagga
     4741 cgtcaccgcg caacacaaca gctcccggca gcggcgtgac atcgggcgca agcgcccgcg
     4801 ccaaaacgga ctttccggtt ccggacagcc cgccgacagc gacaagcgtc ggcgccgaag
     4861 gacggatcgt caggtgcgcg agctcgaaat aggcccgggc cgactgcatg acatcagcct
     4921 tgtcgtcaca ggcgtggcca agacgggcaa gcacgacatt ggcacggatc gcggcgcgca
     4981 gtgacacaaa aagagggagc gctgcgagcg cgtcgagatt ttccagcggt gtcatggcaa
     5041 gatatctgtc gaggagggcg ttcgcagccg cgtgccggtt gtagcgcatg aaatccatga
     5101 ttggaaaggc aagatcgtag agtaaatcaa ccgacgcaat ggtcggatcg aactcaatcg
     5161 cgtcgaacaa gacgggtttg cgctcgatca gtacaatgtt cgccagatgc aggtcgccat
     5221 ggcagcgccg cacgtaaccg tccctgccac gctgtcgcag caactcgcga atccgcagat
     5281 atgccgactg agacgctgcg ttgagatcat cgatatttct ttcggaaaag caggccgccg
     5341 ttcggaacgc cgttgtgttg ccttcgatga atgtcgggat ggattcgacc cagggttcag
     5401 ccgccgcgcg cggcgccacg gcgtgcgaag ccccgatcgc gtcggcaatg gcgttgacga
     5461 gatcggaatc cggctcaccg gcttccgcca gatgatcgat ggtctggcac tcgtcgaagc
     5521 gggtcatttc aaccgcaaac tcgaccggcg tgccgtcgcc gtcgatatca agcgatccat
     5581 cgctatcttg cgtgatcgga acgactcgac gatagatctg cggcgcaaac tggcggttga
     5641 tgttgatctc ttcgtcgcag gcagctttgc gcttctcaag cgtcgagtaa tccaggaacg
     5701 ggaaacggac agcgcgcttg atcttcagcg cgcggtcgcc ttcgagaaag accgacgccg
     5761 catgggtgtc gatccggtgg acctgcggat gcatcgtggg gtcggtcaga aacgcaaaga
     5821 cgctctcctg catgcgagac tccggtggag ttgactttgt catgcgccgg tagccttaag
     5881 gtttgaggac cgcggcaccc aggactcgtc ccgtccgcag ttttttaagg acgtcgttag
     5941 cttcagcaag cgggaacgcc gtcgtctggg tccggattcc ggcctgcgga gcgattttga
     6001 ggaaatccag tccgtcctgg cgcgtgagat tggccaccga cagcagctgc cgttcctccc
     6061 aaagcagatt gtaaggaaag ctcggaatat cgctcatgtg gatgcccgcg cagaccactt
     6121 tgccgccctt gcggaccgcg cgcagcgccg tcggcaccag ttctccggcg ggagcgtaga
     6181 tgatggccgc atcgagtgga gcgtcaggta actcgcccga cgctccggcc cataccgcgc
     6241 cgagccgacg ggcaaaatcc tgcgccgcaa cgtcaccgga tcgcgtgaag gcgtagacgg
     6301 aacggccctg ccagcgcgca atctgggcga cgatgtgccc ggctgcgccg aagccataga
     6361 tgccgagccg ctcggcgtta tcagccatca ccagcgaccg ccagccgatc aggccggcgc
     6421 acaataatgg cgcgatcgaa acgtcgtcgc cgtcttcgcc gagcgggaaa gcatagcgtg
     6481 catcggcaat ggtgtgcgtg gcgaagccgc cgtcccgcgt gtagccggtg aaaagcgggc
     6541 gatcacagag attttccatg ccatccttgc agaagcggca gacgccgcag gtgtatccga
     6601 gccaggggat tccgacccta tcaccgatgc cgtgggtcgt gacatggctg ccgacgaggt
     6661 cgacacggcc tacgatttca tggcccggaa taacaggata tttgatgccg ggaagctccg
     6721 catcgacgac atgaaggtcg gttcggcaaa ccccgcacgc gctcaccttg acgcggattt
     6781 gtccgtcgtc ggggctgggg tccgggcgct cttgcatctg gagaggctcg ccgggggcca
     6841 acagcaccat cgcacgcatc tgttcctccg caagcaacca aacggtgcca ggataatgca
     6901 taccgccggg atcctgggag cccttgacct cgatcaaatg catttaggtg cgataccccc
     6961 tgaaagggaa ggcacccttg tcgtcttgat gaagatcaac gcgggtttcg cgcaagcacc
     7021 taacgtcaga tcaaaggaga gcgcgcatgc cgatcaagga tatcctgttg ccgcttgtcg
     7081 gcgaaccgag tgcggccgcg atcgcggcga ttgacaaatg cgtggcggtg gccggggata
     7141 tcggtgcccg ggttaccgcg atggcagtgg aagaggacat tctagtccgg ccgaaggtga
     7201 cgatctcgga tgacctggat aatacggcgg cggtcgaggc cgtgcgcagc gtatcggacg
     7261 cgcgtggctt gttgaaggcg ttcgacgcgg cggccattcg ctttgccgtc cgcaacgagc
     7321 aaaagcttag ccggctagcg gcggcagata ttgctatgaa tatggcggta tgcgcgcggc
     7381 tcaaggatct gtcgatcttc ccggtcaagc caaacgatga ccgatcggaa aagcttgtcg
     7441 agcggctgat attcgaatcg ggcaggccga tcctgatgtg tccggaggaa ttcgccaacg
     7501 aactggcggt ggtattcgat aaggtcgtga tcgcgtggga ccataccgca ccggccgcaa
     7561 gagcggttgc ggacgcattg ccaatgcttc aggcggctgc gaatgtgcgc attatcacgg
     7621 caaccgacaa gataacgccg gcgaaacagg aatccgggac agcccttgtc agccatctcg
     7681 ccgaacacgg aatcaaggcc accttcgaag cggttaaaat cgagggcagt tcggtcggga
     7741 aggtgttcga ggcatatgtg aaggccaacg ccatcgacct cctgatcatg ggtgcctacc
     7801 gtcactcccg gctgaacgaa atcgtgtcgg gtggcgcgac caagacggtc attgggcggc
     7861 caccatgctg ggtgatgatg tcgcgttaga gcatgatccg gaaaagtggg taccggtttt
     7921 ccgaaaagat catgctcaaa caataggcta gagcgcgatg acgattcatc ctgaaatcat
     7981 cgcgctctag tgtgcaagac gcttagattc ataacatcca ggccggcgcc agccgggcaa
     8041 ttgaactgat gtcgacttac cgtctgaaaa atctcctttc gccgcgttcg ctcgcgctgg
     8101 tcggggccag cccgcgccag gggtccgtcg ggcgggccat tcttgcgaat attcaccagg
     8161 caaaattcaa aggcgaattc gggctggtga attcgcacta tgccgagatc gacggcatcg
     8221 ccgcggtcag cagcctcggc aaattgccgt tcgtgcccga gctcgtggtg attacggcgc
     8281 cggcagctgc catccctgat ctcatcgatg gcgcaggaca gcggggggcg gcaggcgctg
     8341 tcattatcac ctccgggctt ggccatggcg cggggtcgct ggcggagagt gccgaacgcg
     8401 ccgcgcataa atatggcatg cggctgatcg ggccgaattg tcttggcatc atgatgcccg
     8461 gtgcaaacct caacgcaagt ttcgccgccc acatgccgtg tgcgggaaat ctggcgctga
     8521 tctcccagtc gggtgcgatt gccaccggaa tggtggagtg ggcggcgcag cgcgcggtcg
     8581 gtttttccgg aattgtttcc attggtgacc agctcgacgt cgatatcgcc gatctcttgg
     8641 attacttcgc gatggacggc aagacccggg cgatcctgct ctatgtcgag gcgatcaagg
     8701 acgcccgcaa attcatgtcc gcggcgcgcg ccgccgcgcg gatcaagccc gtcgtggtgg
     8761 tcaaatccgg ccgcatggcg cagggtgcga aggccgccgc gacccacacc ggggcgctgg
     8821 ccggttcgga cgcggtctat gatgccgcgt ttcgtcgtgc ggggatcctg cgggtgtcgg
     8881 atcttcgcga gttgttcgac tgcgccgaga cgctggggcg tgtctattcg ccgccgggaa
     8941 agcgactggc gatcctgacc aacggcggtg gcatcggtgt tctcgcgatc gatcggctcg
     9001 ctgaattggg gggaaccccc gcggcgatca cgcctgctac gcgtgagaag ctcgatgcgg
     9061 tcctgccgcc gacctggtcc ggatcaaacc ccgtcgatat cgtcggcgat gccgaccccg
     9121 cgcgttatgc cgcggcactt gaagtattgc tggccgacgc gagcaacgat gcgatcctgg
     9181 tgatgaatgt tcagaccgcg atcgctccag ccggcgacat cgccacgacc gtaaccggtt
     9241 tggtcagcaa gtaccgccag cagcaccgcg actttgccaa gccggtgctc gcggtttggg
     9301 tcggcgcgga ccagaagatc atcgatttgc tgagcggcgc aggtattcca aattatccga
     9361 ccgaagatga tgcggtgcgc ggcttcatgt atctggtgcg gcatcgcgag gtggtggcgg
     9421 agctggctca ggtcccgccc gccatgccca gcgaatttgc gccggatgcc gacgccgcgc
     9481 gcgcaatcgt cgccgcggcg ctcgccgacg gccggcagtg gcttgacccg gtcgagatca
     9541 agcggctgct tgaggcctat gagatcgcga tggtgccgac gtttgcggcc accgacgccg
     9601 agcaggccgt cgctcacgcc tcggcgctat tcgcgcaggg cgcgaccgta gtgctgaaaa
     9661 tcatgtcgcg cgacatcgtt cataagtccg atgtcggcgg cgtcgttctc aatcttacca
     9721 gcaccgatgc cgtgcgtgcg gcgacggccg atatcctcgc gcgggcgaaa gcgcttcgtc
     9781 cggaggcgcg gatatccggc gtcatcgtgc aggcgatgat ggtgcggccg aaggcgcgcg
     9841 aactgatcct cggccttgcc gacgatccga cctttggcac cgtcatcgtg ttcgggcgcg
     9901 gcggcacggc ggtcgagatc atcaacgaca aggcgctggc gcttccgccg ctcgatctgc
     9961 aactggcccg tgacctgatc gagcgcaccc gcgtctcgcg gctgctgcgc gcctaccgag
    10021 atgtgccggc ggtcgagccg gatgccgtcg cgaaggttct ggtcaagctg gcgcagatgg
    10081 ccgcggacat tccagagatc cgtgaactgg acatcaatcc gctgctggcg gatcaagccg
    10141 gagtactggc ggtcgacgcg cgcgtcgcca ttgggcaagc cgagcgaaag tttggcggct
    10201 caggccccgc caacttcgcg gtgcggccat atccgtcgca atggcagcgg cacctcgagg
    10261 tcaaggacgg ctggcgggtc ttcgtgcgcc cgatccgccc cgaggacgaa ccgctgatcc
    10321 atgaattatt gcgacacgtc accagccagg acctgcggct ccggttcttc gcgtcgatga
    10381 aggagttcag ccacgagttc atcgcgcgtt tgacgcagct cgactatgcc cgcgcgatgg
    10441 cctttgtcgc gttggacgaa gccaccaacg aggtcgttgg cgtggtccgg attcactcgg
    10501 attcgattta cgagaacggt gaatacgcga tcctgctcag atccgatctc aagggcaggg
    10561 gactcggttg ggcgttgatg cagctgatca tcgaatatgc caaatccgaa ggactcaagg
    10621 tc
//
LOCUS       DASWGM010000747         7060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1767871,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000747 DASWGM010000000
VERSION     DASWGM010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767871"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..369)
                     /locus_tag="VGA15_20765"
     CDS             complement(16..369)
                     /locus_tag="VGA15_20765"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9590166.1"
                     /translation="MSAKCRCDGDAAGARAFIALLIYATPVVLITHESLAADNNRGAQ
                     LAATCAACHRLDGRDKGIPSIIGLDEGKLAGALQAFKSGERSIMHTVALSLSSEEIAT
                     VVHYLAAQRKETERP"
     gene            complement(425..751)
                     /gene="soxZ"
                     /locus_tag="VGA15_20770"
     CDS             complement(425..751)
                     /gene="soxZ"
                     /locus_tag="VGA15_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX9590167.1"
                     /translation="MFTLVPRVQVPSATAKGEIFQVKALISHQMETGLRHDDQGNVIP
                     RKIINKFACRYNEVVAFKVDFHEAVAANPYVEFYLRATESGRLEFIWEEDGGARYSLA
                     HNLVVS"
     gene            complement(773..1249)
                     /locus_tag="VGA15_20775"
     CDS             complement(773..1249)
                     /locus_tag="VGA15_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEX9590168.1"
                     /translation="MVENTATISLTRRALLTAACVGGAGFAGGTLVSLPSALASDDDV
                     ELIQQLTGKIPTESDRVHLAMPPVFPNGYNVPLALDIDSPMTETDHVRYVRVLAPRNP
                     IIDVATFYFTPQRSQPRVSTRIRIAEPQYILAVAEMNDGALLTAKAWVEVATNGCI"
     gene            1670..3315
                     /gene="groL"
                     /locus_tag="VGA15_20780"
                     /pseudo
     CDS             1670..3315
                     /gene="groL"
                     /locus_tag="VGA15_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492245.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; frameshifted; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
     gene            complement(3969..4328)
                     /locus_tag="VGA15_20785"
     CDS             complement(3969..4328)
                     /locus_tag="VGA15_20785"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9590169.1"
                     /translation="MAASSGSSMSSGHRSFAKEIFYTARQFSADEAVSMGLINRTYSA
                     VDLLTAVNDASTRIGQNAPLTIVAAKLAIDAAQGDSHDKDISAVENAVARCFASEDYK
                     EGRRAFSEKRTPAFRGV"
     gene            complement(4339..4761)
                     /locus_tag="VGA15_20790"
                     /pseudo
     CDS             complement(4339..4761)
                     /locus_tag="VGA15_20790"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
     gene            4827..5360
                     /locus_tag="VGA15_20795"
     CDS             4827..5360
                     /locus_tag="VGA15_20795"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9590170.1"
                     /translation="MIKTRKDFPCPFEEYSCRVLPDWIDANGHMNIAFYGVVFDGGNY
                     AARDALGLGEDYVERAQHGVFAAETHILYIRELHLDDLIRVRTQIVGADDKRLHFAHE
                     IYRDEFSERAAAQEIMYLHVNLKTRRVVPFLDDVRAGIAEATKAHGQLPVPEWCGRRI
                     TSARTPLTRSLERASTS"
     gene            complement(5349..6200)
                     /locus_tag="VGA15_20800"
     CDS             complement(5349..6200)
                     /locus_tag="VGA15_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9590171.1"
                     /translation="MPHIEASGARLYVEETGQGYPIVFVHEFGSDCREWETQVRYFSR
                     AYRCITYNARGYPPSDVPEDINQYGWEFAVDDVAAVMRGLAVERAHVVGLSMGGYAAL
                     QFGLRYPQMASAIVAAGAGSGSPPAQREAWSKETPAIARGFIERGMDAMAQEMANNPT
                     RIQLKYKDPKGWQEFVDHLRQHSEWGMSNTMARYQALRPSLHDFQDQFSRMKVPVLLA
                     VGDEDVPCLETNMMLKTAIPGAGLWICPNTGHAINLEEPAAFNAQVEGFLSAVERGSW
                     RWAYSGS"
     gene            complement(6262..>7060)
                     /locus_tag="VGA15_20805"
     CDS             complement(6262..>7060)
                     /locus_tag="VGA15_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9590172.1"
                     /translation="IAHPAVRRVNFTGSTKVGRIIATLAAKHLKPVLLELGGKAPLIV
                     LDDADIDHAVEATAFGAFMNQGQICMSTERIIVDDKVADAFVAKLAAKAAKLPAGDPR
                     GHVVLGSLVDLAPAERMDVLIADAVAKGAKVVAGGKRSGTVIEATLLDHVTSAMKIYS
                     EESFGPVKSIIRVRGADEAVRVANDTEYGLSAAIFGRDITRALAISRKIQSGICHING
                     PTVADEAQMPFGGMKASGYGRFGGKAVINEFTDLRWITIEGPQHYPF"
BASE COUNT         1468 a         2120 c         2118 g         1354 t
ORIGIN      
        1 gacgcgtccg gcagatcatg gtcgctcggt ttcctttcgt tgcgcggcga gatagtggac
       61 cacggtcgca atctcctcgc tactgagcga aagggcgacg gtgtgcatga tcgaacgctc
      121 gcccgacttg aaggcctgca acgcgccggc aagtttcccc tcgtccagcc caatgatcga
      181 cggaattccc ttgtcacggc cgtcaaggcg atggcatgcg gcgcaagtcg cggcgagctg
      241 ggcgcctcga ttgttgtctg ccgccaggct ttcatgggtg atgagaacga ccggggttgc
      301 gtaaatcagg agcgcaatga aagcgcgcgc acccgccgcg tctccgtcgc accgacattt
      361 tgcactcatg cggcctgcct ctcggcatca aaaagagcag cagaaatcac aggcggcgcc
      421 ggcatcaact gaccacgaga ttgtgcgcca acgagtatcg ggcgccgccg tcttcttccc
      481 atatgaattc gagccggccg ctctccgtcg cccgcaagta aaattcgacg taggggttgg
      541 cagcgaccgc ttcgtggaaa tcgaccttga acgccacgac ctcgttgtag cggcaggcga
      601 acttattgat gatcttgcgc gggatgacgt tgccttggtc atcatgccgc aagccggtct
      661 ccatctgatg gctaatcagg gctttgacct gaaagatttc gcccttcgcc gtagcgctcg
      721 gcacttgtac gcgtggtacc agggtaaaca ttctccgtct cctgccggga tgtcagatgc
      781 agccgttggt ggcaacctcg acccacgcct tggccgtcag cagcgcgccg tcgttcattt
      841 cggctactgc caggatatat tgcggttcgg cgatgcgaat gcgcgtcgac acgcgaggtt
      901 gactgcgctg cggggtgaaa taaaaggtgg cgacgtcgat gatcgggttc cgcggcgcca
      961 gcacgcggac atacctcacg tggtcggttt cagtcattgg gctgtcgata tcgagcgcta
     1021 agggcacgtt atagccgttc ggaaacaccg gcggcatcgc caggtgaacg cgatccgatt
     1081 ctgtgggaat ttttccggtc agctgctgga tcagctccac gtcatcgtcg ctcgcgagcg
     1141 cgctcggcag cgagacgagg gtgccgcccg caaagcccgc accccccaca caggcggccg
     1201 tcaacagggc acggcgcgtc aaacttatcg tcgcggtgtt ttcaaccatg caaaccttcc
     1261 tgccgtccgg cgacgcatgg gcggcagtaa attgtctgcc ggcggatgcg ccacgagggc
     1321 acatcaatcc gatagctccc cggtaacggt cttccgtacg gccaccactc aggcgatcaa
     1381 agaaacgtcc ttggaaccgt tgcgcgctct ttcaggaccc agcagacgcc gatcgtttgc
     1441 aaatttacac gctggcgccg gctgcgtaaa gaattggcgc gcgtattcgt gcgggccttc
     1501 gggttgtttc ttatggcggc gataagagag cttgatgtcg aggctttcct tcgaacgcgg
     1561 ctccgcacta tcgtcgccgg accatggtgc gtcgtttggc cagggcttcc gcgattaggt
     1621 cagggtctcg cgggaacatg aaagcaaaga cttgcttgag gaggaacaaa tgagtgtcaa
     1681 ggaagtcaaa ttctcggtcg aagctcgcga caagatgctg cgcggcgtcg acgtgctcgc
     1741 caatgcggtg agggtgacgc ttgggccgaa gggtcgcaat gtggtgctcg acaaatcttt
     1801 tggcgccccg cgcatcacca aggacggcgt caccgtcgca aaggaaatcg aactcgacga
     1861 caagttcgag aacatgggcg cgcagatggt gcgggaagtt gcctccagga cctcgtacta
     1921 gactagctgg gagatggcac cactaccgca accgtgctcg ctcaagcaat catcaaggaa
     1981 ggagcgaatg cggtggccgc caggatgaac ccgatggatc tcaagcgcgg cgtggatctt
     2041 gccgtggaat ccattgtcgc ggacctgaaa aagaattcaa aaccggtgac atcgaacgag
     2101 gagatcgccc aaataggcag gatctccgcc aacggtgacg ccgaaatcgg acggttcatt
     2161 gccaacgcga tgaagaaggt cggcaacgac ggcgtgatca cggtcgagga ggcgaaatcg
     2221 cttgagaccg agctcgaagt ggtcgaaggc atgcagttcg accgcggcta catatcgcca
     2281 tatttcgtaa ccgacgcgga caagatgcgc gtcgagatgt aagaccctta cttgctgatc
     2341 tacgaaaaga agctatccgc gctgcaggaa ttgttgccgt tgctcgaggc ggtggtgaag
     2401 acggcaaagc cgttgctcat tgttgccgaa gacgtcgaag gcgaagtgct ggcgacactc
     2461 gtcgtcaaca agctgcgcgg tggactgaag gtcgcggcgg tgaaggcgcc gggcttcggt
     2521 gatcgctgca aggcgatgct gcaggatatt gccgttctga ccggcggcac ggtgatctcc
     2581 gaagatctcg gcgtcaagct ggaaaacgtg acgatcgaca cgcccggccg cgccaagaaa
     2641 gtgatgatcg agaaggagaa caccacgatc gtcaagggcg ccggcaggaa ggccggcatc
     2701 gaggcacgga tcgccgagat caaggcacag atcgaggaga cgacctcgga ctatgatcgg
     2761 gagaagttgt aggagcggct cgccaagctc gcgggcggcg ttgcggtgat ccgggtcggc
     2821 ggcgccaccg aagtcgaggt gagggagcgt aaggaccgcg ttgacgacgc gcttcatgcg
     2881 acgcgcgccg cggtcgagga aggcatcctg cccggcggcg gcgtcgcctt gcttcgttcg
     2941 atcaagacca tcgaaggtat caagcgcgaa aatgaggacc agcggcacgg cgtcgagatc
     3001 atcaagaagg cgattagcac accggcgcga cagatcgcca ccaatgccgg cgccgacagc
     3061 tcggtcgttg tcggaaagat cctcgagacg tcgacctatg cctacggcta tgatgcacaa
     3121 tcgggcgagt acggcgacct cgtgcagaag gggattatcg atccgaccaa ggtcgtgcgc
     3181 gcagcgctgc agggggctgc ttcgatcgcc ggcctcatga tcaccaccga ggtgatggtc
     3241 gcggagtggc cgaagcggga ggcgaccccg gctcacccac acgaccaccc gcacgcaggc
     3301 ggcatggatt tctgagccgc tcgcacgtcc acaggcggag gcgcccgaag agctgcccgc
     3361 ctgtccggtg tgtaccgaaa acctaacccc ggacgtctgc gagatttatc gcgctgatga
     3421 agatgtgtac gtggttgcgg ggtgatttga acctgtgagc taagtcattg atcttgttgc
     3481 gacggattcc agcatcaaaa gagaacccca gcaatatatt gatattgcta gggtttttta
     3541 ctaacttggt tgcggggagg cgcaaccatc gataacgaca ttcgctgatg gtgaccattt
     3601 gaggcgccat tggccatggg agagtccgct tcggcgctcg ggacgaacgg atcgtccgtg
     3661 ttgtggaatc aaaatcagcg cggatcttca atgatttcaa cggattttct gacgaaacag
     3721 cggtttctgc ccctagatgg atcaataggt tagacgcgca gtcagaatgg accgcccgag
     3781 aaaatgagcc ctgccaacga cgccacgagc tgcaccacta gtcggcaccg ctccgaaggc
     3841 gacgagatcg aacggaaagc aatttcgcga ttgacgctcg tgcgattgat cgagacactc
     3901 gttcggcgcg gacaacattt tcggtacaca aaaccaaaac cgtgtggcac caaatagacc
     3961 gcaggtctct agacacctct aaatgcgggc gtacgctttt ccgaaaacgc gcgccgtccc
     4021 tccttatagt cttcgcttgc aaaacatctg gccaccgcat tttcgaccgc ggagatatct
     4081 ttgtcatggc tatcgccttg cgccgcatcg attgcaagtt tcgcagccac gatggttagc
     4141 ggtgcattct gtccgatccg cgtcgatgcg tcgttgacgg ccgtaagcag atccacggcc
     4201 gaatacgtgc gattgatgag tcccatcgaa acagcctcgt ccgccgagaa ttgccgcgcc
     4261 gtgtagaaaa tctccttggc gaacgaacga tggcccgacg acatcgacga gccgcttgat
     4321 gccgccatag gcgtagccaa gcccgagcct tgccgccgga atggcaaagc gagaattcgc
     4381 cgcggccaag cgcaagtcgc agcacagcgc cacgccaaga cccccgccga tgcaataacc
     4441 ctggatcatc gcaatcgtcg gcttcgggca cgcaagaatt gcctcgttgg cgaggccagt
     4501 tgcgtcattg tagtcggcga tgccggcctc agaactgcgg accttttcaa actgagagat
     4561 gtccgctccg gaaacgaagg ccttgtcccc ctcgccgcgc agaacgacaa cgcggattgc
     4621 ggggtccccg acgaattccg cgatccgctc aggaatggcc cgccacatct cgtagctcac
     4681 cgcattaagc cgttcggggt tactgaagat cagccatccg gttgccccat ccgtgacggc
     4741 tacaattttt ccttcggaca tttgcgctaa tcctttaaga ctctatacat gtcagcacga
     4801 cgctgcttgg cgagaacggg atgaccatga tcaaaacgcg gaaagatttc ccctgccctt
     4861 tcgaagaata tagctgccgt gtgctcccgg attggatcga cgcaaacggc cacatgaaca
     4921 tcgcctttta tggcgtcgtg ttcgacgggg gaaattatgc tgcccgcgat gctctcggtc
     4981 tgggagagga ttatgtcgag cgcgcccaac acggcgtttt cgcagcggaa acccacattc
     5041 tctacattcg cgaattgcat cttgatgacc ttatcagagt tcgaacgcag atcgtgggcg
     5101 ccgacgacaa gcgtcttcat ttcgcgcacg aaatctatcg cgacgaattc tccgagcgcg
     5161 ccgcggccca ggaaataatg tatctacacg tcaacctgaa aacccgtcgc gtcgttccct
     5221 ttctcgatga cgttcgcgcc ggcatagccg aggcgaccaa agcacacgga caactgccgg
     5281 tgcccgaatg gtgcggccgg agaattacgt ccgctcgcac gcccctgacc cgctcgcttg
     5341 agcgcgcctc aacttcctga ataggcccac cgccagcttc cgcgctcaac tgcgctcaga
     5401 aagccctcga cctgcgcgtt gaaggctgcg ggctcctcca ggttgatcgc atgtccggtg
     5461 ttggggcata tccacaggcc cgcaccgggt atcgccgtct tgagcatcat attggtttcg
     5521 aggcacggca catcctcatc acccaccgcg agcaacaccg gaaccttcat ccgcgagaac
     5581 tgatcctgga agtcgtgaag cgagggccgc agcgcctgat agcgcgccat cgtattcgac
     5641 attccccatt ccgaatgctg acgcagatga tcgacgaact cctgccaacc cttcggatcc
     5701 ttgtatttga gctggatgcg cgtcgggtta ttcgccattt cctgcgccat cgcatccatg
     5761 ccgcgctcga taaagccgcg cgcaatcgcc ggagtttcct ttgaccacgc ctcgcgctgg
     5821 gccggaggcg atccggagcc cgcgccggcg gcgacgatcg cgctcgccat ctgtggatag
     5881 cgcaggccga actgcagggc ggcgtatccg cccatgctca aaccgacgac gtgggcgcgc
     5941 tcgaccgcaa gtccgcgcat cacggcggcg acgtcgtcga ccgcgaactc ccaaccgtac
     6001 tggttaatat cttcgggaac gtcgctcggc ggatatccac gcgcattgta ggtgatgcag
     6061 cgataggcgc gcgagaaata ccggacttgg gtttcccatt cgcggcagtc cgacccgaac
     6121 tcgtgaacaa acacgatcgg atatccctgc ccggtctcct cgacatagag cctcgcgccg
     6181 gaagcttcga tatgtggcat taggaacgct ctcccttgtt ctgcagagca cgcgaaaatt
     6241 ttgccccggg ggacaccccg gtcagaacgg ataatgctgc gggccctcga tcgtgatcca
     6301 tcgcaagtcg gtaaattcgt tgatcaccgc cttgccgccg aagcggccgt aaccactggc
     6361 tttcatgccg ccgaacggca tctgtgcctc gtccgcgacg gtcgggccgt tgatgtgaca
     6421 gatgccgctc tggattttcc tggatatcgc cagggcgcgg gtgatatccc ggccgaagat
     6481 cgccgcggac aatccatatt cggtatcgtt ggcgacgcgc acggcctcgt cggcgccgcg
     6541 gacgcgaatg atcgatttca ccgggccgaa cgattcctcg ctgtagatct tcatcgccga
     6601 cgtcacgtga tccagcaggg tcgcctcgat caccgtgccg ctgcgcttgc cgccggcgac
     6661 aaccttggcg cccttggcga cggcatcggc gatcaggaca tccatgcgct cggccggggc
     6721 gagatcgacc agggagccca gcaccacgtg accgcgcgga tcgcccgccg gcagtttcgc
     6781 ggccttcgct gcaagttttg cgacaaacgc gtcggcgacc ttgtcgtcga cgatgatgcg
     6841 ctcggtcgac atgcagatct ggccctggtt catgaacgca ccgaaggctg tcgcctccac
     6901 ggcgtggtcg atatccgcgt cgtcgagcac gatcagcggc gccttgccgc cgagttcaag
     6961 cagcaccggc ttcaagtgct tggcggcgag ggttgcgatg atcctcccca ccttagtgga
     7021 gccggtgaaa ttgacccggc gaaccgccgg atgcgcgatc
//
LOCUS       DASWGM010000748         2598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_623577,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000748 DASWGM010000000
VERSION     DASWGM010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623577"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..425
                     /locus_tag="VGA15_20810"
     CDS             57..425
                     /locus_tag="VGA15_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590173.1"
                     /translation="MSDTDRILDSAISPDQRLVPSTATRLQPNRGRGSQAAARISDPP
                     LSEVSALIKRVEDILLAFVLLVAAAPLMLLVGIAIKHESRRSPRFDLSHRPENRDHPA
                     ADRAAVKCRRPPFCPSYGIW"
     gene            complement(448..2031)
                     /locus_tag="VGA15_20815"
     CDS             complement(448..2031)
                     /locus_tag="VGA15_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9590174.1"
                     /translation="MSPPSNLDDLSPAELKALVLALLGEVSALKEVVAEQRAEIARLK
                     GLKGPPSIKPSGMDKASDAKPPAGGKKRRGRGRKRIARVAIEDRVLKAEVPAGSRFKG
                     YESFLVQDLVLRPVAIRFRRERWATPDGCTIVAPLPPGIEGHFGPDLRRFVLAQSHQG
                     QVTVPRLVALLEAIGIEVSKRQVVRLLIGRQGRFLGEAREVLRAGLAGAAWVTVDDTG
                     ARHKAANGFCTHIGNDRFAWFGTTGSKSRLNFLELLRAGHGDYAINAEALAYMRSRAL
                     AGPVIRQLAEHDGKRFADRAAWQEHLDRLGITALKALPDPVPIATEGALWGSVKAHGL
                     LNDAVIVSDDAGQFNLGRHALCWVHAERLVHKLDTFTDQHRAAQQRLRHLIWWFYSDL
                     KAYCRDPTPLRKAALRARFDRIFKRRTGFATLDRLLARLHANKPELLMVLDRPEIPLH
                     TNGSENDIRCQVTKRKVSGGTRSDAGRDCRDAFLGLAKTCAKLGISFWDYLGARLIIA
                     GNPAIPYLPQLVAQRCSTA"
     gene            2015..2362
                     /locus_tag="VGA15_20820"
     CDS             2015..2362
                     /locus_tag="VGA15_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590175.1"
                     /translation="MLGGDMPPQNPNFAVTGIAHLQGESICRAPQRARPFAPVTKKAL
                     MMPLYQSQIGLSGQLGCFPSRFSVVSVFGANTYLSRTLKNALLDEITLRWFEQLNDAI
                     AVFKIRLKRVQDL"
BASE COUNT          478 a          826 c          808 g          486 t
ORIGIN      
        1 tcgggttgag tcagatatag gaagtgtatc acgagtcgag gaataagagc gcatcaatga
       61 gcgacacgga tagaatactt gattccgcta tttcgcctga tcaacggctt gttccgtcta
      121 ctgcgacccg gttacagccg aatcgagggc gcggctcaca ggctgctgcc cggatttccg
      181 atcctccgct gagcgaagtg tcggcgctca taaagcgcgt cgaagatatc ctgctggctt
      241 ttgtcctcct cgtcgccgcc gcaccattaa tgcttttggt gggcatcgcc atcaagcatg
      301 aatcgcgccg atcaccccga ttcgatttgt cgcaccggcc cgaaaaccgc gatcatccgg
      361 ccgcggaccg cgcggccgtc aaatgccgcc gcccgccctt ttgcccctct tacggaattt
      421 ggtaacaggg gcaaaaggcc gggcgggtta ggctgttgag caacgctgag cgacgagttg
      481 cgggaggtag gggatggctg ggttgccggc gatgatgagc ctggcgccga ggtagtccca
      541 gaacgagatg ccgagcttgg cgcaggtctt ggcgaggccg aggaaggcat cgcggcagtc
      601 gcggccggca tcgctgcgcg tgccgccgct gaccttgcgt ttggtcactt ggcagcggat
      661 gtcgttttca gagccgttgg tgtggagcgg gatctccggg cgatcgagca ccatcagcaa
      721 ttccggcttg ttggcgtgca gccgagcgag caggcggtcg agggtggcaa agccggtgcg
      781 gcgtttgaag atgcgatcaa agcgggcgcg caaggcggcc ttgcgcagtg gtgtgggatc
      841 gcggcaatag gctttgagat cgctatagaa ccaccagatc agatggcgca gccgctgctg
      901 ggcggcgcgg tgctggtcgg tgaaggtgtc gagcttgtgc accagccgct cggcgtggac
      961 ccagcacagc gcatgccggc cgagattgaa ctggccggca tcgtcgctga cgatgacggc
     1021 atcgtttagc aggccatggg ccttgacgct gccccagagt gcgccttccg tagcgatcgg
     1081 caccggatcg ggcagagcct tgagcgcggt aatgccaagt cggtcgagat gctcttgcca
     1141 cgccgcccga tcggcgaagc gcttgccgtc gtgctcggcg agttgccgga tcaccggacc
     1201 ggcgagcgcc cggctgcgca tataagcgag cgcctcggcg ttgatcgcgt agtcgccatg
     1261 gccggcgcgc agcagttcga gaaagttgag ccggctcttc gagccggtcg tgccgaacca
     1321 ggcaaagcgg tcgttgccaa tatgcgtgca gaacccgttc gccgccttgt ggcgcgcccc
     1381 ggtgtcgtcg accgtgaccc aggcggcacc ggccagaccc gcgcgcaata cctcgcgcgc
     1441 ctcgccgagg aaacgcccct ggcggccgat caacagccgc actacctgcc gcttggagac
     1501 ttcgatgccg atcgcttcca ggagcgccac gagccgcggc acggtcactt ggccctgatg
     1561 agactgcgcc agcacgaagc ggcgcaggtc tggcccaaaa tgaccctcga tgcccggcgg
     1621 cagcggcgcc acgatggtgc agccgtcggg cgttgcccag cgctcgcgcc ggaaccggat
     1681 ggcgaccggc cgcagcacca gatcctggac caggaagctc tcatagccct tgaaccgcga
     1741 gccggccggc acctcggcct tgagcacccg atcctcgatc gccacccgcg cgatccgctt
     1801 gcggccgcgg ccccggcgct tcttcccgcc ggccggcggc tttgcgtcgc tcgccttatc
     1861 catgccggaa ggcttgatcg aaggcgggcc tttcagcccc ttcagccgcg caatctccgc
     1921 gcgctgctcc gccaccacct ctttcagcgc cgaaacctcg cccagcaatg caagcaccag
     1981 cgccttcagc tcggcaggag agagatcatc aagattgctc ggcggcgaca tgccgccaca
     2041 gaatcccaac tttgccgtga cgggaatcgc tcacctgcaa ggtgagtcga tttgccgcgc
     2101 ccctcagcgt gcccggcctt ttgcccctgt tactaaaaaa gcgttaatga tgccgctgta
     2161 ccaatcgcaa atcgggctat cagggcagct cggctgtttc ccctcgcgct tctcggtcgt
     2221 cagcgtattt ggggccaaca cctacctgtc gaggactctc aaaaacgcac tcttagatga
     2281 aatcactctt cgctggtttg agcagctcaa tgacgccatc gcggtattca agatcaggct
     2341 gaagcgcgtt caggatctct gacaattcct cgcgcgtgat tcgcaaggcg tgattgggcg
     2401 cgtagtggag ttcaacagct tccaacgtct tcgggtagaa ctggctctca gctactcgat
     2461 agccgcaggt accagaacga cgaatgattt taacttccat cggacgcacc ccccagaata
     2521 catcagcacc cgaacgcacg cagtatgcag ccactgcttt ggtccggcga tgttaaattc
     2581 gtgacaactt ccattatc
//
LOCUS       DASWGM010000749         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2457953,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000749 DASWGM010000000
VERSION     DASWGM010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2457953"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1232
                     /gene="dnaN"
                     /locus_tag="VGA15_20825"
     CDS             114..1232
                     /gene="dnaN"
                     /locus_tag="VGA15_20825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX9590176.1"
                     /translation="MKVTVERAQLMKSLGHVHRVVERRNTIPILGNVLIRAENAKLSL
                     KATDLDLEVTETLAAESATGGSTTVPAHMFYDIVRKLPDGAQIVLEGDGDRSVLAIRA
                     GRSRFTLQTLPENDFPDLAAGDMTHSFTLPASDMKRLIDRTQFAISTEETRYYLNGIY
                     LHSAGSAKDPTLRAVATDGHRLAQIDLALPKGASGMPGVIVPRKTVGEVLRLIEDNEA
                     EVAIELSQGKIRFTIGNVVLTSKLIDGTFPDYGRVIPQNNDKELIVDKKDFEAAVDRV
                     STISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYAAEALDIGFNSRYLLDIA
                     AQIEGEVAVLRLADPGSPTLVQDKDSKGALYVLMPMRV"
     gene            1322..1693
                     /locus_tag="VGA15_20830"
     CDS             1322..1693
                     /locus_tag="VGA15_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX9590177.1"
                     /translation="MLQAPHHRPTAKRIRSFAKKMRREPTDAEAAMWRLLRHRRLALF
                     KFRRQVPLQGFIVDFVCFEKRMIVEIDGSQHASSERDAARDAVLMAESFRIARYWNND
                     VLQQPSAVLEDILAKLAEDDR"
     gene            1776..>1915
                     /locus_tag="VGA15_20835"
     CDS             1776..>1915
                     /locus_tag="VGA15_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9590178.1"
                     /translation="MTASRIHRLTLTHFRNYRAASLEARGDVVVLVGPNGAGKTNCIE
                     AIS"
BASE COUNT          374 a          623 c          578 g          340 t
ORIGIN      
        1 ccccctgcgg gtttgatcaa atccagctat gatcggcttt tcgggtttca aacgccgcgg
       61 cgcgccttcc gggccgcccg gcttttccat cttaggatca ggcgggtatt gcaatgaagg
      121 ttaccgtcga acgcgcgcaa ctcatgaaat cgctgggcca cgtccatcgc gtggtcgagc
      181 gccgcaacac cattccgatc ctcggcaacg tgctgatccg cgccgaaaac gcgaagctgt
      241 cgctgaaggc caccgacctt gacctcgagg tgacggaaac gctggcggcg gaaagcgcaa
      301 ccggcggttc gaccacggtg ccggcgcaca tgttctacga catcgtgcgc aagctgcccg
      361 atggcgcgca gatcgtgctg gaaggcgatg gcgaccgctc ggtgctggcg atccgcgccg
      421 gccggtcgcg cttcacgctg cagaccctgc ccgaaaacga tttccccgat ctcgccgccg
      481 gcgacatgac gcattcgttc acgctgcccg cctccgacat gaagcggttg atcgaccgca
      541 cccagtttgc gatctcgacc gaggaaaccc gttactatct caacggcatc tatctgcact
      601 ccgccggcag cgccaaggac ccgaccttgc gcgcggtcgc gaccgacggg catcgtctcg
      661 cgcagatcga tctggctttg cccaaaggcg catccggcat gcccggcgtc atcgtcccgc
      721 gcaaaaccgt cggcgaagtg ttgcggttga tcgaggacaa cgaggccgaa gtcgcaatcg
      781 aactgtcgca gggcaagatc cgcttcacca tcggcaatgt cgtgctgacc tcgaaactga
      841 tcgacggcac gtttcccgac tacggccgcg tcatcccgca aaacaacgac aaggaactca
      901 tcgtcgacaa gaaagatttc gaggccgcgg tcgaccgcgt ctcgaccatc tcgagcgagc
      961 gcggccgcgc ggtgaagctg gcgttgtccg ccggcaagct ggtgctgtcg gtgaccaacc
     1021 cggattccgg cagcgcgacc gaagagcttg aggtcgaata cgccgccgag gcgctcgata
     1081 tcggattcaa ttcgcgctat ctgctggaca tcgccgccca gatcgagggc gaagtcgcgg
     1141 tgctgcgact ggccgatccg ggttcgccga cgctggtaca ggacaaggat tccaaaggcg
     1201 cgctctacgt gctgatgccg atgcgggtgt agaaatttcc cgcaagccgc cccctgatct
     1261 cccctctccc cttgcgggag agggtggcgc cgaacgaagt tcggcgacgg gtgaggggtt
     1321 cgtgctgcag gcgccacacc atcggccaac cgccaagcgc atccgcagtt tcgcaaagaa
     1381 gatgcgccgc gagccaacgg acgccgaagc ggccatgtgg cgcttgctac gccatcgacg
     1441 gcttgcgctc ttcaaatttc gccggcaagt cccccttcag gggtttattg tcgacttcgt
     1501 ttgctttgaa aagcgcatga tcgtcgagat cgatgggagt cagcatgcgt cgtcggagcg
     1561 cgacgcagcg agagatgccg tccttatggc cgaaagtttt cggatcgcgc gctactggaa
     1621 caatgacgtg ctgcaacaac cttctgccgt tttggaggac atcctcgcaa agctcgccga
     1681 agacgataga taacccctca cccgtcgccg aacttcgttc ggcgccaccc tctcccgcaa
     1741 ggggagaggg gaagaaagac cgacaccgcc cttccatgac cgcctcccgc atccaccgcc
     1801 tgacgctgac gcacttccgc aattatcggg cggcgagcct ggaagcgcgc ggtgatgtgg
     1861 tggtgctggt cggcccaaac ggtgccggaa agaccaactg catcgaggcg atttc
//
LOCUS       DASWGM010000750         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2597504,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000750 DASWGM010000000
VERSION     DASWGM010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2597504"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VGA15_20840"
     CDS             <1..307
                     /locus_tag="VGA15_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX9590179.1"
                     /translation="DLAVLHHRVPVTIEPDDFERWLDCRDDDAEKVMALLVAPGEGRF
                     VWHEISTRVNHAANDDAQLILPITAEEMAAEQPRPTKKNPRKPAQVASEDDGQGSLF"
     gene            complement(479..1060)
                     /locus_tag="VGA15_20845"
     CDS             complement(479..1060)
                     /locus_tag="VGA15_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase"
                     /protein_id="HEX9590180.1"
                     /translation="MTVSDRIRVKDVRVLSDNHYILKTTTFEWRRNSGEWQTQARETY
                     DRGNGAALLPYNLAQRSVVLVRQFRYPAYVNGCDDLLIEAAAGLLDNESPEVRILAEA
                     EEETGYRLGKVQKVFEAFMSPGAITEKLHFFVAEYEAGMRVGSGGGIASEGEDIEVLE
                     LPIDSALAMIGDGRIVDAKTIMLLQYAALNIFR"
     gene            complement(1082..1537)
                     /locus_tag="VGA15_20850"
     CDS             complement(1082..1537)
                     /locus_tag="VGA15_20850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEX9590181.1"
                     /translation="MNSRTPSDALTIKPIEDGDIAEVIALWQRCGSTRAWNDPAGDIA
                     LARKETNATVLLGRNDSALVASVLVGHDGHRGWVYYVTVDPDHRFKGYGRVIMTAAEN
                     WLRARGIEKLQLMVRGDNAKVRAFYESLGYYDQERVVFAKWLDGREPTP"
     gene            complement(1534..>2174)
                     /locus_tag="VGA15_20855"
     CDS             complement(1534..>2174)
                     /locus_tag="VGA15_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9590182.1"
                     /translation="SDIAVDFSVRHGIDIRDPLEAKHPWYVLMELSSSRDDARAALES
                     ILAKGLDEGIIDDAVIAANLSQRAAFWKLRDEMSAAQKPEGGSIKHDISVPVAAVPDF
                     IAEANAAVVKLIPGSRPVPFGHLGDGNIHYNVSQPVGAEPADFLERWHEVNAVVFAIV
                     MRMGGSISAEHGIGVLKRDELPEVKDKVAIELMRGIKAMLDPHGIMNPGKVL"
BASE COUNT          416 a          672 c          655 g          431 t
ORIGIN      
        1 tgatctcgcg gtattacatc accgcgtgcc cgtgaccatc gagcccgatg atttcgagcg
       61 ctggctcgat tgccgtgatg acgatgcaga gaaggtaatg gcgctgctgg tcgcgcccgg
      121 cgaaggccgt ttcgtctggc atgagatttc gacgcgggtc aatcacgccg ccaatgacga
      181 cgcgcaactg atcctcccca tcacggcgga agagatggca gcggagcaac cgaggcccac
      241 aaaaaagaac ccacgcaaac cggcgcaagt ggcgtctgaa gacgacgggc aggggtcgtt
      301 gttttgattt cgtagggttg tcgacgacga aattcgcgtc gtcccggcga acgccgggac
      361 ccataatcac aggccgtcgt attgcgcgat gccggagcga caatcccctt aacaatgaac
      421 cgcggtggct atgggtcccg gcgtgcgccg ggacgacatg tctattggtc gttgcgcgtc
      481 atcgaaaaat attcagcgcc gcgtattgca agagcatgat ggttttggca tcgacgatgc
      541 ggccgtcgcc gatcatggcc agcgcagaat cgatcggcaa ttccagcacc tcgatgtctt
      601 cgccttccga ggcgatgccg ccgccgcttc cgacccgcat acccgcctca tattccgcga
      661 caaagaagtg cagtttctcg gtgatcgcgc cggggctcat gaaggcttcg aatacctttt
      721 gcaccttgcc gagccggtag ccggtttctt cctcggcctc ggcgagaatt cgcacttccg
      781 gagattcgtt gtccaatagt cccgccgcgg cctcgatcag gagatcgtcg cagccgttga
      841 cataagccgg atagcgaaac tgccgcacca gcaccacgct gcgctgcgcc aggttgtagg
      901 gcaacagcgc cgcgccgttg ccgcggtcgt aggtttcgcg ggcttgggtc tgccattcgc
      961 cgctgttgcg ccgccactcg aaggtggtgg tcttcaggat gtaatgattg tcggagagca
     1021 cacggacatc tttcacgcgg atgcggtcgg aaacggtcat gtgattccca ttgatttttg
     1081 attacggtgt cggctcgcgg ccgtcgagcc acttggcaaa gaccacgcgt tcctgatcgt
     1141 agtagccgag cgactcgtag aaggcgcgga ccttggcgtt gtccccacgg accatgagct
     1201 ggagcttttc aataccgcgc gcacggagcc aattttcggc ggcggtcatg atcacacgac
     1261 cgtagccctt gaagcgatga tcagggtcga cggtaacgta atagacccag ccgcgatgac
     1321 cgtcatgtcc gaccagcacc gacgcgacga gggcgctgtc attccgaccc aacagcaccg
     1381 ttgcgttcgt ttccttgcgc gccagcgcaa tatcaccagc aggatcgttc cacgcgcgcg
     1441 tcgacccgca acgctgccac agcgcaatca cttcggcgat atcgccgtct tcaattggct
     1501 tgatggtcag ggcgtcactg ggggttcgag aattcacagc accttgcccg gattcatgat
     1561 gccgtgcggg tcgagcatcg ccttgatgcc gcgcatcagc tcgatcgcga ccttgtcctt
     1621 gacttcaggg agttcgtcgc gcttgagcac gccgatgccg tgctcggccg agatcgatcc
     1681 gcccatccgc atcacgatcg cgaacaccac cgcattgacc tcgtgccaac gctcgaggaa
     1741 atccgcgggc tcggcgccaa cgggctggct gacattgtaa tggatattgc cgtcgccgag
     1801 atggccgaac ggcaccggcc gcgagcctgg aatcaatttt accacggcgg cgttggcttc
     1861 ggcgatgaaa tcgggcaccg cggcaaccgg caccgaaata tcgtgcttga tcgagccgcc
     1921 ctccggcttc tgcgccgcgg acatttcgtc gcgcagtttc cagaatgcag ctcgctggct
     1981 gaggttcgcc gcgatcaccg cgtcatcgat aatgccctcg tcgagcccct tggccaggat
     2041 cgattccagc gcggcgcggg cgtcgtcgcg cgaggacgac agttccatca gcacgtacca
     2101 gggatgcttg gcttcgaggg gatcgcggat gtcgatgccg tgacgcacgc tgaaatccac
     2161 cgcgatgtcg gaaa
//
LOCUS       DASWGM010000751         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1768469,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000751 DASWGM010000000
VERSION     DASWGM010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768469"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1766)
                     /locus_tag="VGA15_20860"
     CDS             complement(<1..1766)
                     /locus_tag="VGA15_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9590183.1"
                     /translation="MGVEGIGASVVRKEDRRFITGRGRYVDDIKLQGMTFAQFVRSPH
                     AHAKVKSIDSSEAMKMPGVVGILTGQQIVDDKVGNLICGWAITSKDGTPMKMGAWPAM
                     APETVRFAGQAVAVVIAETKNQAKDAAEAVVIDYEVLPAAADISAAIKPGAPQLHPEA
                     PGNVIYDWAIGDEAAVKDAFSKAANVVALDLTNNRLVPNAMEPRAAIAEYNEAEEHFT
                     LYTTSQNPHVARLVLSAFYNIAPEHKLRVVAPDVGGGFGSKIYIYPEEMVALWASKKV
                     RRPVKWTGDRSEAFLTDAHGRDHITKAEMAFDKDNKIIGLRVKTHANLGAYMSLFSSA
                     VPTYLYATLLSGQYNIPAIYAEVVTVYTNTVPVDAYRGAGRPEASFLLERLMETAARQ
                     LKVDPAELRRKNFITSFPHQTPVIMAYDIGDFGASLDAAMKAIDYAGFPARKAKAKSD
                     GKLRGIGFSCYIEACGIAPSKAVGSLGAGVGLWESAEVRVNPVGTIEILTGSHSHGQG
                     HETTFAQLVAERLGVPISQVQIVHGDTDKVQFGMGTYGSRSIAVGGTAIIKAMEKVEA
                     KGKKIAAHLLEASEGDIVIENG"
     gene            complement(1854..>2001)
                     /locus_tag="VGA15_20865"
     CDS             complement(1854..>2001)
                     /locus_tag="VGA15_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9590184.1"
                     /translation="VHRHSGGLDEDTVRHELEGNICRCTGYHNIVKSVLDAAGRMKVS
                     QAAE"
BASE COUNT          333 a          645 c          610 g          413 t
ORIGIN      
        1 tcgccgttct cgatgacgat gtcgccctcg gacgcctcca agagatgcgc cgcgattttc
       61 ttgcccttgg cttcgacctt ctccatcgcc ttgatgatcg cggtgccgcc gaccgcgatc
      121 gagcgcgaac cataagtgcc catgccgaac tgcaccttgt cggtgtcgcc gtggacaatc
      181 tgcacctggc tgatcggaac gccgagccgc tctgcgacca gttgcgcaaa cgtcgtctcg
      241 tgcccctggc cgtggctgtg cgaaccggtg aggatttcga tggtgccgac cggattgacg
      301 cggacctccg ccgattccca caagccgaca ccggcgccca ggctgccgac cgctttcgac
      361 ggcgcgatgc cgcaggcctc gatatagcag gagaaaccga tgccgcgcag cttgccgtca
      421 gacttggctt tggccttgcg tgccggaaag ccggcgtagt cgatcgcctt cattgccgca
      481 tccagcgatg cgccgaaatc gccgatgtcg taggccatga tgacaggggt ctggtgcggg
      541 aagctggtga tgaaattctt gcggcgcaat tccgccggat cgaccttcaa ttgccgcgcc
      601 gccgtttcca tcagccgttc gagcaggaaa ctcgcctcgg gccggcccgc gccgcggtag
      661 gcatcgaccg gtacggtgtt ggtatagacc gttaccacct cggcatagat cgccgggata
      721 ttgtactggc ccgacagcag cgtcgcgtag agataggtcg gcaccgccga cgagaacagc
      781 gacatatagg cgccgagatt ggcgtgggtt ttcacgcgca ggccaataat cttgttgtcc
      841 ttgtcgaagg ccatctcggc cttcgtgata tgatcgcgac catgggcgtc ggtgaggaag
      901 gcctcggagc gatcgccggt ccacttcacc ggacgccgca ccttcttgga ggcccacagc
      961 gccaccatct cttcgggata gatgtagatc ttggagccga agccgccgcc gacatcgggc
     1021 gcgaccaccc gcagcttgtg ttccggcgcg atgttgtaga atgccgacag cacgagccgg
     1081 gcgacatgcg gattctgcga ggtcgtgtag agggtaaaat gctcttcggc ttcgttgtat
     1141 tccgcgatcg ccgcgcgtgg ctccatcgca ttcggcacca gccggttatt ggtgaggtcg
     1201 agcgccacca cattggccgc cttgctgaag gcgtccttga cggcggcctc gtcgccgatc
     1261 gcccagtcgt agatcacgtt gccgggtgct tccggatgca gctgcggcgc gccaggcttg
     1321 atcgccgcgc taatatcggc ggctgcggga agcacttcgt agtcgatcac gacggcttcc
     1381 gccgcgtcct tggcctggtt cttggtctcg gcaatcacca ccgcgaccgc ctgtccggca
     1441 aagcgcaccg tctccggcgc catcgccggc cacgcgccca tcttcatcgg cgtgccgtcc
     1501 ttggaggtaa tcgcccagcc gcaaatgaga ttgccgacct tgtcatcgac gatctgttgt
     1561 ccggtcagta tcccgaccac gccgggcatt ttcatcgcct ccgacgagtc gatgctcttc
     1621 accttggcgt gcgcgtgggg gctgcggacg aactgcgcga aggtcatgcc ctgcagcttg
     1681 atgtcgtcga cgtagcgacc tcggcccgtg atgaaacgcc ggtcttcctt gcgcacgacg
     1741 cttgcgccaa taccttcaac gcccatgtcc tgatctccct gccggagttt tattgtccgc
     1801 catttccatc gaaattcggc ggtctaaatc tgataatcga acgtcggcga cggctattcg
     1861 gccgcctgcg aaaccttcat gcggccggcg gcgtcgagca ccgatttcac gatattgtgg
     1921 tagccagtgc agcggcagat attgccctca agttcgtgcc ggacggtgtc ctcgtcgaga
     1981 ccgccgctgt ggcggtggac g
//
LOCUS       DASWGM010000752         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_200460,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000752 DASWGM010000000
VERSION     DASWGM010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200460"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VGA15_20870"
     CDS             <1..1364
                     /locus_tag="VGA15_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX9590185.1"
                     /translation="VRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPCVSG
                     CGTIRVDYGRGTMSIGSRTFKTGDVITIDGSLGQVLAGRMPMIEPKLSGEFGTLMGWA
                     DSVRKLGVRVNADTPDDARTAIKFGGEGIGLCRTEHMFFEETRIRTVREMILAEDEQA
                     RRAALSKLLPMQRADFVELFEIMKGLPVTIRLLDPPLHEFLPHTQAEIEEVARAMNTD
                     PRRLADRARDLAEFNPMLGFRGCRLAIAYPEIAEMQARAIFEAAVEAEKRTGKAVGLE
                     VMVPLIATKMEFDLVKARIDATALSVMKATGKKLAYQVGTMIELPRACLMAGDIARTA
                     EFFSFGTNDLTQTTYGISRDDAAGFLGTYIANGILEIDPFISVDRDGVGELVKIGVAR
                     GRKTRPDLKVGICGEHGGDPASVAFCHEIGLDYVSCSPYRVPIARLAAAQAALGKAVA
                     SQA"
     gene            1759..3147
                     /locus_tag="VGA15_20875"
     CDS             1759..3147
                     /locus_tag="VGA15_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEX9590186.1"
                     /translation="MSVMRNRSKGARFAPFGLGLCVFALIPTEIGYQDIASLLARQPG
                     VAERWQKRVSAAASTIQVATYSFGRPIGTALPQPPTYLLARLDNQGIDITGSLTRNPI
                     TKAPPRYQASDFPKVDRTLKGDRLVIVRPEPAAPANAAPSNEDPSTSNALVAGAKTAA
                     AMPPAATAPLDPELAEALKAPPLDGSKMAPDATSPRDGFSVKTASLFFGRSSIGAAES
                     MERWQPGEEPTIITPGAGPDPDMKMLAPPPSAADEPSRAIESGESVVAKGEVNSDNQR
                     LTPAERLGLFDEKARAKSEKCLAEAVYFEARGEAVRGQIAVAQVVMNRAFSGFYPTTV
                     CAVVYQNKHRHLACQFTFACDNVADVVREPDMWDRAKKIAKAMLDGQLWLPEVGKSTH
                     YHAYWVRPSWVNEMKKMYKFGVHTFYRPRAWGDGSDAPSWGTPAQTAVISAELSEAAK
                     SSAELSDTTVRR"
     gene            3162..3887
                     /locus_tag="VGA15_20880"
     CDS             3162..3887
                     /locus_tag="VGA15_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590187.1"
                     /translation="MNLGTLGPTVQRGGGPPLPRYPGKGSTPTIVPESNVVSTIIIVA
                     GRHNCADDECPKLQAGCDNLISMSEGKMTDSGTGDKVGPTLAIKPLTECSAGELIRLT
                     SGAWAIVANDSSARRIFVISGDDAPLTYVLPEDSTEACLSYGTGFRVASVHASFVGMH
                     TYGHEGFDPVGKLIVARPYAHDGRTSRYFAAPAGQPRFLDLENFQTVSEPLGHRALFK
                     DWEVSISRPGHPEPVAVVKSPVH"
     gene            complement(3897..>4283)
                     /locus_tag="VGA15_20885"
     CDS             complement(3897..>4283)
                     /locus_tag="VGA15_20885"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019045.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEX9590188.1"
                     /translation="FNHRFGLDDAILIKDNHIAVAGGVRPVLARARANVGHLVKIEIE
                     VDTLSQLREVLDTGLADVVLLDNMDIADLTEAAKLADGRVVLEASGSITQSSIAKIAA
                     TGVDYVSSGALTHSAPNFDVALDIDA"
BASE COUNT          811 a         1331 c         1362 g          779 t
ORIGIN      
        1 tggtgcggat cgaaaccagc ccggaagaca ttcacggcat gcacgccgcc gaaggcattc
       61 tgaccacgcg cggcggcatg acctcgcacg ccgcggtggt cgcgcgcggc atgggcaagc
      121 cctgcgtctc cggctgcggc accatccgtg tcgactatgg ccgcggcacc atgagcatcg
      181 gctcgcgcac cttcaagacc ggcgacgtca tcaccatcga tggatcgctg ggccaggtgc
      241 tggccgggcg gatgccgatg atcgagccga aattgtcggg cgaattcggc accctgatgg
      301 ggtgggccga ttccgtccgc aagctcggcg ttcgcgtcaa cgccgacacg ccggacgatg
      361 cgcgcaccgc cataaaattc ggcggcgagg gtatcgggct ttgccgcacc gagcacatgt
      421 tcttcgagga gacccggatt cgcaccgtgc gcgagatgat tctcgccgag gacgaacagg
      481 cgcgccgcgc cgcgctgtca aaactgttgc cgatgcagcg cgcggatttc gtcgagctgt
      541 tcgagatcat gaagggtctg ccggtgacga tccggctgct cgatccgcca ttgcatgagt
      601 tcctgccgca cacccaggcc gagatcgagg aagtcgcgcg cgcgatgaat accgatccgc
      661 ggcggctggc ggatcgcgcc cgcgatctgg ctgaattcaa tccgatgctc ggcttccgcg
      721 gctgtcgtct cgccatcgcc tacccggaga tcgccgagat gcaggcgcgc gccatcttcg
      781 aggccgcggt cgaagcggaa aagcgcaccg gcaaggcggt cggcctcgag gtgatggtgc
      841 cgctgatcgc gaccaagatg gagttcgatc tggtcaaggc gcggatcgat gcgaccgcgc
      901 tgtcggtgat gaaggcgacc gggaaaaagc tcgcctatca ggtcggcacc atgatcgaac
      961 tgccgcgcgc ctgcctgatg gccggcgaca ttgccaggac ggcggagttc ttctcgttcg
     1021 gcaccaacga tctgacgcag accacttatg gcatcagccg cgatgacgcc gcggggtttt
     1081 tgggcaccta catcgccaac ggaatcctgg aaatcgatcc gttcatttcg gtcgatcgcg
     1141 acggcgtcgg cgagttggtg aagatcggcg tcgcccgcgg gcgcaagacc cgccccgatc
     1201 tgaaggtcgg catttgcggc gagcatggcg gcgatccggc ttcggtcgcg ttctgccacg
     1261 agatcggact ggattacgta tcgtgctcgc cctaccgcgt cccgatcgcg cggcttgccg
     1321 cggcacaggc ggcgctcggc aaggcggtcg ccagccaggc gtaacgatca gctatcggtt
     1381 ttaagacgca aatggccggg attttcccgg ccattttctt gtttgcgagg cgcgcaaaaa
     1441 tgcgggccgc ggcatgcctg ccgtggtccg tgcgagcacg ggtgtgccgt aatgacgcgg
     1501 cggaaaaatt tgcagggtgc caagaatttt cttcatcaac ttcgttcagg ccatatttac
     1561 cactttgttg atgtgcctgt ttaccaacgt ttttgatgcg attgtgcaaa gtccagcgag
     1621 ttcttgcgtg tgtcgcgtgc gcaacgccga ttaactccct cgcaacctaa attcgatatc
     1681 accaaaaaag atcgaattgc acggatgtac tgggttcgat cgtgcgtctg tattgagtag
     1741 tgttgcgtga gcgtaccgat gtcagtaatg cgtaaccggt cgaagggcgc gaggttcgcg
     1801 cccttcggtc tcggtctttg cgtcttcgca ttgatcccga ccgaaattgg atatcaggac
     1861 attgcatcgc tgctggcccg gcagccgggc gtcgccgagc gctggcagaa gcgggtatcc
     1921 gccgccgcca gcaccatcca ggtcgccact tacagcttcg gccgtccgat cgggacggct
     1981 cttccgcagc ccccgaccta tctgctggcg cgcctcgaca atcagggcat cgacatcacg
     2041 ggttcgctga cgcgcaatcc gatcacgaag gcgccgccgc gctatcaggc ctccgatttc
     2101 cccaaggtcg atcgcaccct gaagggcgac cgcctcgtca ttgtccggcc cgagcccgca
     2161 gcgcccgcga acgcggcacc gtcgaacgag gatccgtcga cgtccaatgc cctggtggcc
     2221 ggcgcaaaga ccgccgccgc gatgccgccg gccgcaacgg ctccgctcga tccggaactt
     2281 gccgaagcgc tgaaagctcc gccgctcgac ggttcgaaaa tggcgcccga tgccacatcg
     2341 ccgcgcgatg gcttctcggt gaaaaccgcg agcctgtttt tcggcagatc gtcgatcggc
     2401 gcggccgaga gcatggagcg ctggcagccc ggcgaagagc cgaccatcat aacaccgggc
     2461 gccggtcccg atccggacat gaaaatgctg gcgccgccgc cgtcggccgc ggatgagccg
     2521 tcgagggcga tcgagagcgg cgagagcgtc gtcgccaagg gcgaagtcaa ttccgacaac
     2581 cagcgtctga cgccggccga acggctcggc ctgttcgacg aaaaggcacg cgccaaatcc
     2641 gagaaatgcc tcgccgaagc cgtctatttc gaagcccgcg gcgaagccgt gcgcggccag
     2701 atcgcggtgg cgcaggtggt gatgaaccgc gcgttctccg ggttttaccc gaccacggtg
     2761 tgcgccgtgg tctaccagaa caagcaccgt cacctcgcct gccagttcac cttcgcctgc
     2821 gacaacgtcg ccgacgtggt gcgcgagccc gacatgtggg accgcgccaa aaagatcgcg
     2881 aaagcgatgc tcgacggcca gctgtggttg ccggaagtcg gcaagtcgac gcattaccac
     2941 gcctattggg tgcggccgtc ctgggtcaat gaaatgaaga agatgtacaa attcggcgtg
     3001 cacaccttct accggccgcg cgcctggggc gacggcagcg acgcgccgag ctggggaacg
     3061 ccggcgcaga cggcggtgat ttccgccgag ctctcggaag ccgcgaagag ttcggcggaa
     3121 ctgtcggata cgacggtgcg gcgctaaaga tcgattagct agtgaacttg ggtacgcttg
     3181 gcccgactgt tcagcgcggc ggcggaccgc cgttgccgcg atatcccggc aaaggttcaa
     3241 ctcccacaat tgtgcccgaa tcaaatgttg tctcgacgat cattatcgtt gcaggcaggc
     3301 acaattgcgc agatgacgaa tgcccaaaat tacaagcagg ctgcgacaac ctcatcagta
     3361 tgagtgaggg caaaatgact gacagcggga cgggcgacaa ggttgggccg actcttgcca
     3421 tcaagccgct gacagagtgc agcgcgggcg aacttattcg cctgaccagc ggcgcctggg
     3481 caattgtagc gaacgattcg agcgcgagga gaattttcgt gatttccggt gatgatgcgc
     3541 cgctgaccta tgtcctcccg gaggacagca cggaggcgtg tctaagctac gggaccggat
     3601 ttcgagtggc gtcagtacac gcaagcttcg tcgggatgca tacctatggc catgaggggt
     3661 tcgatcccgt tggaaagctg atcgttgccc ggccgtacgc gcacgatggg cgcaccagcc
     3721 gctatttcgc ggcgccagct ggccaacccc ggtttcttga ccttgaaaat tttcagaccg
     3781 tctcggaacc gcttggacat cgcgcattgt ttaaggattg ggaagtgtcg atcagcaggc
     3841 cgggacaccc cgagccggtc gcggtcgtca agtctccggt tcactagagt gctcgcttac
     3901 gcgtcgatgt cgagcgcgac gtcgaaattc ggcgccgaat gcgtcagcgc gccggacgag
     3961 acgtagtcca caccggtcgc ggcgatcttc gcaatcgagc tttgagtgat gctgccggaa
     4021 gcctccagca ccacgcgtcc gtccgcgagt ttagcggcct cggtcagatc ggcaatatcc
     4081 atattgtcca gcagcaccac gtcggcgagg ccggtgtcga gcacctcgcg caactgcgag
     4141 agcgtatcga cctcgatctc gatcttgacc agatgtccga cgttcgcccg cgcgcgcgcc
     4201 agcactggcc tgacgccgcc ggcgaccgcg atgtgattgt ccttgatcag gatcgcatcg
     4261 tcgagaccga agcggtgatt gaa
//
LOCUS       DASWGM010000753         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2034681,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000753 DASWGM010000000
VERSION     DASWGM010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034681"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VGA15_20890"
     CDS             complement(<1..920)
                     /locus_tag="VGA15_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX9590189.1"
                     /translation="MPSPFEVAHAPIAANAMSGSIRLAQTQPAQAPAPSPAQPAADAT
                     TDAQAPGDEPIGNVAAVTGIATVIRNKDSVPLKLKDDIFLNDVLQTSANSSLGVTFND
                     ATTFHLSANARITVDSYVYEDGGQQNAALFDVAKGTVAFVAAAVAKTGDMKISTPTAT
                     MGIRGTTGLIEVPEGASATSRNNVAIKLYPDPDGKVGRIEVNDRAGARIGFLTQGASG
                     FAIRGGGGMRVAAVPLTISPQQALRDQGFVRQVHSAQNVGRQVVTEQRALRRANPALN
                     NPNRTNQPRQPGPQRQNGLPQRPGSPQPGQP"
     gene            1310..1822
                     /locus_tag="VGA15_20895"
     CDS             1310..1822
                     /locus_tag="VGA15_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590190.1"
                     /translation="MLLLGGTLNAHADSDVYDDITKHPRGDDVLQADTDDCSRMLGAP
                     QTGTPTSQSYKNCMRGRGWRYSHTVRERATRDRMNPIRIIPGRCARILRSAVSPDRTA
                     RIFEVRGRGVARRPPCMKTPGGWNGPGRRASQNPGCGEKALEHAEYDGAEKGDCQIRG
                     HNAQFADERT"
     gene            2019..3236
                     /locus_tag="VGA15_20900"
     CDS             2019..3236
                     /locus_tag="VGA15_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX9590191.1"
                     /translation="MTRGVRLYLVGSFVLVSLAGCGRGFFQSAEREPWRAEAEIACLK
                     SGAVKESAELVRISPISGPGVCGAEYPLKVAALGESSGSFGFADGLRPSTDIGNQPRW
                     PMSRPPPSQPQALPYPDTAMRQPGYGAAPNAPISLTAPGVAPEEDDIDLPPEGSPASR
                     EPIARTPYPSPQANPPRDRTSAPAYSERPSAVPPLPRLGPPQGNSVTAFGPVAVKPVA
                     TLACPIVSVLDRWLADSVQPAAQRWFGARVVEIKQISAYSCRGMNGSSRAHISEHAFG
                     NALDIAGFTLADGRHISVKDGWKGVPEEQGFLHDVQAAACQQFSTVLAPGSNVYHYDH
                     IHVDLMRRASQRVICEPAAMSGAEVAARAGQRNPFASRAPFVTGSLDGSKAVAHRPKS
                     SGKVNEEDEFEDE"
BASE COUNT          686 a         1180 c         1266 g          684 t
ORIGIN      
        1 ggctgtcccg gctgcggcga acccggccgc tgcggcaaac cattttggcg ctgcgggccc
       61 ggttgacgcg gctggttggt gcggttcgga ttgttcagag ccggattggc gcgacgcaag
      121 gcgcgttgct cggtgaccac ctgccggccc acattctgcg cggagtggac ctggcgcaca
      181 aatccctgat cgcgcaacgc ctgctgcggc gagatcgtca gcggcaccgc ggcgacgcgc
      241 atgccgccac cgccccggat cgcaaagccg ctggcgccct gggtgagaaa accgatccgc
      301 gcgccggcgc ggtcattgac ctcgatccgg ccgaccttgc cgtccggatc gggataaagc
      361 ttgatcgcga cattgttccg gctggtggcc gacgcgcctt caggcacctc gatcaggccg
      421 gtggtgccgc gaatgcccat cgtcgcggtc ggtgtcgaga ttttcatgtc gccggtcttg
      481 gcgacggcgg cggccacgaa ggccacggtg cccttggcga cgtcgaacag cgcggcgttt
      541 tgttggccgc catcctcata gacataactg tcgaccgtga tccgcgcatt ggcggagaga
      601 tgaaaggtgg tggcgtcatt gaaggtgacg ccgagcgatg agttcgccga ggtctgcagc
      661 acgtcgttga gaaagatatc gtccttgagc ttgagcggaa ctgaatcctt gttgcggatg
      721 acggtcgcga tgccggtcac ggctgcgacg ttgccaatcg gttcatcgcc gggggcttgc
      781 gcatcggtgg tcgcgtcagc cgccggctgg gcgggcgagg gtgccggcgc ctgtgccggt
      841 tgcgtctgcg ccagcctgat cgagccggac atcgcgttcg cggcgatcgg cgcgtgtgct
      901 acttcgaatg gagacggcat cgcgagaaca ttcgccgttc cggccagcat gaaacaggcc
      961 acaagccgga ccgcgaagga aaccagatgc cgcgtcacga tggagccccc aggaaggaat
     1021 cgaattggcg atggcagttt tgcgattgag ggcccggctg gctgaatatg ggatgaatgt
     1081 cggcggcatt gttgccctgc cagcaccaaa cgatcaagcg cccgcgtctg catttgcgcg
     1141 aatgacgccc gctacaagac gcggttggcg cgctacccgt ggtgagcgca ctcacgtttc
     1201 caatttgtaa tcgtttcgat ccgaaccggc gctcgttcgg tgcgaccgat agccgaggcg
     1261 gcggtcgcga cgatggagat cgaagatgaa aagcttgagt ccgatcgcca tgctgctgct
     1321 cggcggcacg ctgaacgcgc atgccgacag cgacgtctat gacgacatca caaaacatcc
     1381 gcgcggcgat gacgtgctgc aggccgatac cgatgattgc tcacggatgc ttggcgcgcc
     1441 ccaaaccggt acgccgacct cgcagtcata caagaactgc atgcgcggac ggggctggcg
     1501 ctacagccac acggtgcgcg agcgcgcaac gcgagaccgc atgaacccga tccggataat
     1561 cccgggccga tgtgcaagga ttttacgatc ggcggtatca ccggatcgga ctgctcgaat
     1621 ttttgaagtg cgcgggcgag gcgtcgcgcg gcgaccacca tgcatgaaaa cgcccggcgg
     1681 ctggaatggt ccaggccgcc gggcgtctca aaatccaggt tgtggtgaga aggccctaga
     1741 acacgccgag tacgatggcg ccgaaaaagg cgattgccag atacgcggtc acaacgctca
     1801 gtttgctgac gaaaggacct aagaaggtca tctgggaagt ctccctggtt taggtcgctg
     1861 cccactaacg cggaaagtag caaacggttc cggccggata aagtcagccc tgctgcacta
     1921 tcgcgaaatc tccgcgccga accgccggca acgtggttaa agattcgtga aaacaaggcg
     1981 ttgatgcgtc cttaaagaaa ttcgtcgatc ctgcgcggat gacgcgcgga gttcgtttgt
     2041 atctcgtcgg ctctttcgtc cttgtctcgc tggcgggttg cggtcgcgga ttttttcagt
     2101 ccgcggagcg cgaaccgtgg cgggccgagg ccgaaattgc gtgcctgaag tccggcgcgg
     2161 tcaaagagag tgccgaactg gtccggatca gcccgatttc gggtccgggg gtttgcggcg
     2221 cggagtatcc gctcaaggtc gcggcgctcg gcgaaagcag cggcagtttt gggtttgccg
     2281 acggcctgcg cccgtccacg gacatcggca atcagccgcg ctggccgatg tcgcggccac
     2341 ctccatctca gccccaggcc ctgccttatc ccgacaccgc gatgcggcag cccggctacg
     2401 gtgccgcgcc caatgctccg atttcattga cggcgcccgg cgtggcgccc gaggaagacg
     2461 acatcgatct gccgccggag ggctcgcccg cgtcgaggga gccgatcgct cgaacgccgt
     2521 atccgagccc gcaagccaat ccgccgcgcg accgcacctc tgctcccgct tattcggaac
     2581 gccccagcgc cgtcccgcct ttgccacggc tcggccctcc gcagggtaat tccgtcacgg
     2641 cattcgggcc tgttgcggta aagccggtgg cgacgctggc ctgtccgatc gtttcggtgc
     2701 tcgatcgctg gctcgccgat tccgtgcagc ccgcagcaca gcgctggttc ggcgcccgcg
     2761 tcgtcgagat caagcagatc tcggcctatt cctgccgtgg catgaacggc tcttcgcgcg
     2821 cgcatatttc cgaacacgct tttggcaatg cgctggatat cgcaggcttc acgctcgccg
     2881 acggacggca catttcggtg aaggatggct ggaagggcgt gccggaagaa cagggctttc
     2941 tgcacgacgt gcaggcagcc gcctgccagc agttcagcac cgtgctggcg ccgggctcca
     3001 acgtctatca ctacgatcac atccacgtcg acctgatgcg ccgcgccagc caacgcgtga
     3061 tctgcgagcc ggcggcgatg tcgggcgcgg aggttgccgc gcgcgcgggc cagcgcaatc
     3121 ccttcgcctc gcgtgcgccg ttcgtgacgg gatcgctcga cgggagtaaa gccgtggcgc
     3181 atcgacccaa gtccagtggc aaggtcaacg aagaagatga gttcgaggac gagtagagtg
     3241 taaggcagcc ccaagagcgc ttcaatcgtt ccccggatgc cgcgcagcgc gccgctcttg
     3301 cggcgtggtg cgctgctgat ccggggtcca ccttgcaagt gataggtccc ggctctgcgg
     3361 agcagcgtga aggacgctgc accgcgtccg ggacacgaga ggagcactcg ccgatcgtcc
     3421 ggtgatttgc ccgtcgggcg gtttgttgac aggcgtgtcc agccctcttt gcaaaaatat
     3481 ttcgcttccg tggtcggtag aaacatctct atgagtctcg ccatcccgtc ccacagagag
     3541 gggcgtatcg cgatcgtcac ggacgtaggg cacgggatgc ggtggacgcg gcagcgtttt
     3601 gcgcgcgatg ggatcgcagg gcgggttctt tgaatccgtg agcggtcaac agcacgccga
     3661 cgagagatgc tggccgcgga cggcaaaatc gtgtggtcct gacgccccga cgctggcgtc
     3721 aagtacgcgg agttgcgtcg gcccaaccgg gcttagacaa aacctatccg ctgacgacgg
     3781 tggcaaaaga gcccggtcac cggggagagc acgaca
//
LOCUS       DASWGM010000754         2850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_94742,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000754 DASWGM010000000
VERSION     DASWGM010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94742"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VGA15_20905"
     CDS             <1..1252
                     /locus_tag="VGA15_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9590192.1"
                     /translation="MQNGGCHPIDKCLGPHPFLGAKFHYLAASTVEKSTGKTVFPPYE
                     IKQFDGIPVAFIGLTLKGTPDIVSPVGVAGLEFKDEADTVNALVPELKAQGVEAIVVL
                     IHEGGFPAGDYNECPGISGPIVDIIRKFDHAVDVVISGHTHQAYVCDIDGRLVTSGDK
                     FGTIVTTIDLKLDPATRDVVSAKADNLIVRTGVYAKDPEQTALLAAYDKVAAPIANRA
                     AGSVTDALSRTPNDAGESPLGDIIADAQLAATSAETDGGAVIAVTNPGGIRTDIPKRD
                     DSAVSYADVFASQPFRNQLVTLTLTGMQIKTMLEQQWLDPKRPRILQVSKGFSYTWDG
                     ARPNGDRVVADRMSLNGQRIGPATSYRVTVNNFLSVGGDGFTVLKEGTAPRFGVYDVD
                     ALYAFFRANSPIGPAPADRIVRMN"
     gene            1353..2282
                     /locus_tag="VGA15_20910"
     CDS             1353..2282
                     /locus_tag="VGA15_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX9590193.1"
                     /translation="MTTLLLTHPATLDHLTPPGHPERPDRIRAVAEVLAEDRFGKLVR
                     GEAPEGSLDTVTLCHNEHYVEELRHIAPMSGLIYLDGDTSMSPGTWEAVMRGVGGAVA
                     ATEAVMSGAHHNAFVAIRPPGHHAEINKPMGFCFFDNAAIAARHAQRKYGIARAAVVD
                     FDVHHGNGTQDIFWADPTVMYCSTHQMPLFPGTGASGERGEHDNIVNAPLASEDGSAK
                     FRAAFENVILPQLQKFAPELIIVSAGFDAHYRDPLASLNLKSEDFGWVTRKLMDVADS
                     SAGGRIVSVLEGGYDLQGLKESAAAHVTALMGG"
     gene            2357..2608
                     /locus_tag="VGA15_20915"
     CDS             2357..2608
                     /locus_tag="VGA15_20915"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX9590194.1"
                     /translation="MAEDTQVDVKKLSFERAMEELETIVTRLEGGKVPLEESVAIYER
                     GESLKRRCEELLRQAEARVDKITTDANGQVTGAEPLDVQ"
BASE COUNT          577 a          920 c          856 g          497 t
ORIGIN      
        1 gatgcagaac ggcggctgcc atcccatcga caaatgtctg gggccgcacc cgttcctcgg
       61 cgccaagttt cactacctcg cggccagcac ggtcgagaag agcaccggca agaccgtgtt
      121 cccgccctac gagatcaagc agttcgacgg cattccggtt gccttcatcg gcctgacctt
      181 gaagggaacg cccgacatcg tctcaccggt gggcgttgca ggtctcgagt tcaaggacga
      241 ggccgacacg gtgaatgcgc tggtgccgga actgaaggca cagggggtcg aggcgatcgt
      301 cgtgctgatc catgaaggcg ggtttccggc cggcgattat aacgaatgcc cgggcatatc
      361 cggcccgatc gtcgacatca tcaggaaatt cgaccacgcc gtcgacgtgg tgatcagcgg
      421 ccacacccat caggcctatg tctgcgacat cgacggccgg cttgtcacca gcggtgacaa
      481 gttcggcacc atcgtcacaa caatcgatct gaagctcgat cccgcgaccc gcgacgtcgt
      541 cagcgccaag gccgacaacc tcatcgtccg caccggcgtc tatgccaaag atccggaaca
      601 gaccgcgctg cttgccgcgt acgataaagt tgccgcgccg atcgccaacc gcgcggcggg
      661 gtcggttact gacgcgctgt cgcgcacgcc caacgatgcc ggcgagagcc cgctcggcga
      721 tatcatcgcc gatgcccagt tggccgcgac cagcgccgag acagatggcg gcgccgtgat
      781 cgccgtcacc aatccgggcg gcatccgcac cgacattccg aaaagagacg atagcgcggt
      841 gagctacgcc gacgtcttcg ccagccagcc gttccggaat cagctggtga cgctgacgct
      901 gacgggaatg cagatcaaga ctatgctcga gcagcaatgg ctcgatccga aacgaccacg
      961 gattctgcag gtctcgaagg gcttcagcta tacatgggac ggcgcccggc cgaatggcga
     1021 ccgcgtggtc gccgatcgca tgtcactcaa cgggcaacgc atcggacccg cgacgagcta
     1081 tcgcgtgacc gtgaacaatt ttctatccgt cgggggcgac ggctttaccg tgctgaagga
     1141 aggcaccgcg ccgcgttttg gcgtctacga tgtcgatgcc ctgtacgcct ttttccgggc
     1201 aaacagcccg atcgggcccg ctccggccga tcgcatcgtg cgaatgaact gaattgcaaa
     1261 gccatatcga cgggcccagc tcttcccgag cccgcgttga ccctctagat tacttggtcc
     1321 tcacaagcgc gttcgacgtg ccaaggtatt gaatgacgac gcttcttctc acacatccgg
     1381 caaccctcga ccacttgacc ccgccgggac atccggaacg acccgatcgg attcgcgcgg
     1441 tcgccgaagt gctcgcggag gaccgcttcg gcaagctcgt gcgcggcgaa gcaccggaag
     1501 gcagtcttga tacggtcacg ctctgtcaca acgagcatta cgtcgaggaa ctccggcaca
     1561 tcgcccctat gagcggtttg atctatctcg acggcgacac gtcgatgtct cccgggacct
     1621 gggaagcggt gatgcgcggc gtcggcggcg cggtcgccgc caccgaggcg gtgatgtcag
     1681 gcgcccacca taatgcgttc gtcgcgatcc gccctccggg ccatcacgcc gagatcaaca
     1741 agccgatggg cttttgtttc ttcgacaacg ccgccatcgc cgcgcgccac gcccagcgca
     1801 aatacggcat agcccgggcc gccgtggtcg atttcgacgt gcatcacggc aacggcaccc
     1861 aggatatttt ctgggccgat ccgacggtga tgtattgctc gacgcaccag atgccgctgt
     1921 ttcccggcac cggcgccagc ggcgagcgcg gcgaacacga caatatcgtc aacgcgccgc
     1981 tggcctccga agacggcagc gccaaattcc gcgccgcctt cgaaaacgta atcctgccgc
     2041 aattgcagaa atttgcgcct gagctgatca tcgtctccgc cggcttcgac gcgcactacc
     2101 gcgatccgct ggcgtcgctc aatctcaaat cggaagattt cggctgggtc acgcgcaagc
     2161 tgatggacgt cgccgattcg agcgccggcg gccgaattgt ctcggtgctc gagggcggct
     2221 atgacttgca gggcctcaag gagtcggccg cggcacacgt cacggcattg atgggcgggt
     2281 gaatgcccgc cacaatacct ccgcaaaatt gtcttcgaat ccgcgccggc gaaggggcgc
     2341 atcccgggaa agcgatatgg ctgaagatac ccaagtggac gtcaagaagc tgagcttcga
     2401 acgcgcgatg gaagagcttg aaaccatcgt cacgcggctc gaaggcggca aggtgccgct
     2461 ggaggaatcc gtcgccatct acgagcgggg cgagtccttg aaacggcggt gcgaggaatt
     2521 gctgcggcag gcggaggcgc gggtcgacaa gatcaccacc gacgccaatg gccaggtgac
     2581 cggtgcggag ccgctcgacg ttcaatgacc ccacgacctg ttggacgccg atgcacgtcg
     2641 ccccccccgg taacattaga gaatatttga cgaaattagc cgaattcgac gccaagcgac
     2701 ccggacgggg tctgaaatag ctcttggcgc gttacccggc ctgttatagt ccccgccgtc
     2761 agagccccat ttttcgaagt tcgcgatacc ttgcggcatc ctctgccacg aacttcgaaa
     2821 aacggggcat tcgtaactct atattctagt
//
LOCUS       DASWGM010000755         2820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1038888,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000755 DASWGM010000000
VERSION     DASWGM010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038888"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VGA15_20920"
     CDS             <1..516
                     /locus_tag="VGA15_20920"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590195.1"
                     /translation="GIDAGPGFLSMSGERDPQGALEAIPDSLWDKVIDTNLTSMFTTI
                     RAAARPMKRQNSGRIILTASIAGVRPGAIVGMPYQISKAGVAHLAKQSALELARYNIL
                     VNAILPGPFLTQLTTPELQKRFEAGSPLHRVGRPEEIQGLALFLASPASSYITGTHIV
                     IDGGALLGRAD"
     gene            553..1341
                     /locus_tag="VGA15_20925"
     CDS             553..1341
                     /locus_tag="VGA15_20925"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590196.1"
                     /translation="MKADALFDLSGHVVAVTGAASGLGLAMSEVLAMNGAHVVMADID
                     RKALDVAAEGLRAAGCKVEMTLTDVSARGEVAAAFDEIVGRHGRLDCVFANAGVTGGP
                     GFGNPEGEIETASLELWEQSLRVNLSGAFFTMRAAAKHMKRQRSGSIVVTSSVAALRA
                     SSLPSYGYHAAKAGIAQAVRVAALELGPYNVRVNAIAPGPFATNIANGRLRTPEGAAM
                     FSKTLPMARVADVKEIKGLALLLASQASSFITGAIIPIDGGTQA"
     gene            1385..2632
                     /locus_tag="VGA15_20930"
     CDS             1385..2632
                     /locus_tag="VGA15_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9590197.1"
                     /translation="MINARVCVLAVAGAIVSCSSVHADDTFRIGLIDPLSGPAATTGE
                     VGLKTWQFLAGEVNASGGLNGRKLEVVGYDNKMNPQETAIQAQKAIDSGVRLLVRANG
                     SAPGVALNDFLNKFNERNPSKQALYFDYAGADPAATNEKCSYWQIRWAANIDMKVLAL
                     ASFLKGQADTKKLYLFNGDYSTGQGIQKAMRANMAEKRPDVQIVGDELVPLMKVNDFA
                     PYVEKIKASGADTVVTGAWGQDLALLLKAAGEAGLKVKWYTFFAQGIGSPTAVRQSGL
                     PPHTVFQIYEAHANLPSAAYREEEKNFRAVVGAGQTMMYPGSVNAMDMFAKAARELKT
                     LDVAKIVASLEGAKFKPRAGGDGWIRAEDHQVFQPLYVASIGPIEKDKGFDEENTGWG
                     WRQIGEVKPEDTLVPTTCQMKRP"
     gene            2709..>2820
                     /locus_tag="VGA15_20935"
     CDS             2709..>2820
                     /locus_tag="VGA15_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590198.1"
                     /translation="MLQLVTFSILNGLLYGMLLFLLASGLTLIFSMMGVLN"
BASE COUNT          584 a          815 c          906 g          515 t
ORIGIN      
        1 gggatcgacg ccggccccgg ctttctgtcg atgagcggcg agcgcgatcc gcagggtgcg
       61 ctggaggcga tacctgacag cctgtgggac aaggtgatcg ataccaacct gacgtcgatg
      121 ttcacgacga tcagggcggc agcccggccg atgaagcggc aaaacagcgg gcgcattatc
      181 ctgacggcgt cgatcgcggg tgttaggccc ggcgcgatcg tcgggatgcc gtatcagatc
      241 tccaaagcgg gtgtcgccca ccttgccaag caatcggcgc ttgaactcgc ccgctacaat
      301 atcctggtga acgcaattct gccggggcct tttctgacgc agctgacaac gccggaattg
      361 cagaagcgtt tcgaggccgg cagccccctg catcgcgtgg gacggccgga agagatccaa
      421 gggctggcgc tgtttctggc gtcgccggca tcgagctaca tcacggggac tcatatcgtc
      481 atcgacggcg gtgcgctgct tgggcgtgcg gactaaccgc tcccgggtcc ggttcgaacg
      541 taaaggattg agatgaaggc cgacgcatta ttcgatcttt ccggccatgt cgtcgcggtc
      601 accggcgccg cctccggcct tggcctggcg atgtccgaag ttcttgccat gaacggggca
      661 catgtcgtca tggcggatat cgatcgcaag gcgcttgatg tggccgccga agggctacgc
      721 gccgcgggct gcaaggtcga aatgacattg accgacgtga gcgcgcgggg cgaggttgcg
      781 gccgcattcg acgaaatcgt tggtcgccat ggacggctcg attgtgtctt cgccaatgcc
      841 ggcgtcaccg gtggccccgg attcggcaat cccgaagggg agatcgaaac ggcatcgctg
      901 gaactatggg agcaatccct tcgcgtcaat ctgagcgggg cattcttcac gatgcgggcg
      961 gcggccaagc acatgaagcg ccagcgaagc ggatcgatcg tcgtgacgtc ttcagtcgcg
     1021 gcactcaggg catcgagcct gccgagctat ggctatcacg cagcgaaagc cgggatcgcg
     1081 caggcggtcc gcgtcgccgc gctcgagctt gggccataca atgtacgcgt aaatgcgatc
     1141 gcgcccggac cgttcgcgac caacatcgcc aatggccggc tccgcacgcc ggagggcgcc
     1201 gcgatgttct caaagacctt gccgatggcg cgcgttgccg acgtcaagga gatcaagggg
     1261 ctggcgctgt tgctggcctc gcaggcatcc agcttcataa ccggagcgat cattccgatc
     1321 gatggcggga cacaggcctg aaccaattgg aatccgcgat aagtacaaaa ggggagaaac
     1381 caaaatgatc aatgcaagag tatgcgttct tgctgttgcc ggcgcgatcg tatcctgcag
     1441 ctccgtgcac gccgatgaca cattccggat cggcctgatc gacccgctct cgggtccggc
     1501 tgcgaccacc ggcgaggtcg ggctcaagac ctggcagttt ctcgccggtg aggtcaatgc
     1561 aagcggcgga ctcaatggtc ggaagctcga agttgtcggc tatgacaaca agatgaaccc
     1621 gcaggaaacc gcgatccagg cgcaaaaggc gatcgactcc ggcgtccggt tgctggtgcg
     1681 tgccaatggt tcggcgcccg gtgtcgcgct caatgatttt ttgaacaagt tcaacgagcg
     1741 gaatccaagc aagcaagccc tgtatttcga ttatgccggt gccgaccccg cagcaaccaa
     1801 tgagaagtgc agctattggc agatccgatg ggccgcgaat atcgacatga aggtgctcgc
     1861 gctcgcgagc tttctcaagg gacaggccga tactaagaag ctttatttgt tcaatggaga
     1921 ttattcgaca ggccagggca tccaaaaggc catgcgggcc aacatggctg aaaagcgtcc
     1981 cgacgtccaa atcgtcggcg acgaactggt gccgctgatg aaggtcaatg acttcgcgcc
     2041 ctatgtggag aagatcaagg cttccggggc tgacacggtc gtcaccggcg cgtggggaca
     2101 ggatctcgcg ctgctgctca aggcggccgg cgaagccggc ctcaaggtga agtggtatac
     2161 attcttcgcg cagggcatcg gcagccccac cgccgtccgc cagagcggac tgccgccgca
     2221 cactgtcttc cagatctatg aagcacacgc caatcttccg tcggcggcct accgcgagga
     2281 ggaaaagaat tttcgcgcag tggtcggcgc cggccagacc atgatgtatc ccggatccgt
     2341 caatgcgatg gatatgttcg cgaaggcggc gagggaactg aagaccctcg acgtcgccaa
     2401 aatcgttgcc agcctggaag gcgcgaagtt caagcctcgc gcgggcggag acggatggat
     2461 ccgcgccgag gatcatcagg tgtttcagcc gctctatgtg gcatcgatcg gcccgatcga
     2521 gaaggacaag gggttcgacg aggagaatac ggggtggggc tggcgccaga tcggcgaggt
     2581 caagccggag gatacccttg tgccgaccac gtgccagatg aaacgtccgt aataagaatg
     2641 acggggcagg cggccttgtg ccgcctgcaa ccgacggaag ctccgatagc ggtcaaaagc
     2701 caggaatcat gctccagctc gtcacatttt caattctgaa cggactgctt tacggcatgc
     2761 tgctgtttct gctcgccagc ggcctcacgc tgatattcag catgatgggt gtgctgaata
//
LOCUS       DASWGM010000756         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1178458,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000756 DASWGM010000000
VERSION     DASWGM010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178458"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VGA15_20940"
     CDS             complement(<1..685)
                     /locus_tag="VGA15_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX9590199.1"
                     /translation="MTVYSGPVFDMAVNQFGVIANHLSIPMDERDRLLQPKRAITVSC
                     PIHRDDGTILVFEGYRVQHHLTLGPTKGGTRFAPSVDIGEVAALAIWMSWKCALVGLP
                     YGGAKGGVRVDLATISKRELEALSRRYMQEMIPFVGPHTDVMAPDMGTNEQVMAWFMD
                     TYSMYQGRTVTEIVTGKPVASGGTLGRLEATGRGVAHLAKRVMKELAIDLNNGTAVIQ
                     GFGNVGSYAA"
     gene            905..>1795
                     /locus_tag="VGA15_20945"
     CDS             905..>1795
                     /locus_tag="VGA15_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX9590200.1"
                     /translation="MQALFIGQTYIDVTFITDHMPTGDEKHVASAYAVSFGGNAVTAA
                     FCCAKLGIVPDLIATVANDWLGRMFQDMSAKYGISIHPRKVNSSSLSFIMPKDGKRAI
                     VRCRDDEHIHPFPLLNLGGCRALHIDGHQPDAAIHYAKLCREDGILTSLDGGGLRTNT
                     HELLEFIDVAIVAERLCEQMDLTPEKMLDYLKSRGCRVGGVTQGERGLLWYDEAGAVR
                     TMPALPISHERVLDTNGAGDVFHGAYVFSYLNNPGKSWHDHFEFARAASTYKIQRLGN
                     EAGLPTLADIENVKQEFQIGR"
BASE COUNT          354 a          573 c          534 g          334 t
ORIGIN      
        1 gcgcggcata ggagccgaca ttgccaaaac cctggatcac cgcggtgcca ttgttcaggt
       61 cgatcgcgag ctccttcatc acccgcttgg cgagatgcgc gaccccgcgc ccggtggctt
      121 cgagccggcc cagtgtgccg ccggaagcca ccggcttgcc ggtgacgatc tcggtgacgg
      181 tgcggccctg atacatggaa taggtgtcca tgaaccaggc catcacctgc tcattggtgc
      241 ccatgtccgg cgccatcacg tcggtatgcg gtccgacgaa gggaatcatc tcctgcatat
      301 agcgccgcga cagcgcctcc agctcgcgtt tggaaatcgt cgcgagatcg acccgaacgc
      361 cccccttggc gccgccatag gggaggccga ccagcgcaca cttccagctc atccagatcg
      421 caagtgctgc cacctcgccg atatcgacac tcggcgcgaa acgcgtgccg cccttggtcg
      481 ggccgagggt aagatgatgc tgcacgcgat aaccctcgaa caccaagatc gtgccgtcgt
      541 cgcgatggat cggacaggag acggtgatcg cccgtttagg ctgcaacaga cggtcgcgct
      601 cgtccatcgg gatcgacaga tgattggcga tgacgccgaa ctggttcacc gccatatcga
      661 ataccgggcc ggaatagacc gtcatcgtct ctcctttatc tctctgtcag gaactgtgaa
      721 ccgccgctgg ccgtcggaaa tgcgacgcac tggcgatgtg ggaccacggt cgtagtcccg
      781 ccacacggtt atctcaagcg attaagcggg tgattcggcg aataatttta gcgaatctcc
      841 ggcgcgaagc ggtgctattg tggggtgccg ccgggatcac cattagcgca tgacggatga
      901 ctttatgcag gccctcttca tcggacaaac ctatatcgac gtcaccttca ttaccgacca
      961 catgccgacc ggcgacgaga agcacgtggc gtcggcctat gcggtctcgt tcggcggcaa
     1021 cgccgtcacg gcggctttct gctgtgccaa gctcggcatc gtgccggacc tgatcgccac
     1081 ggtggccaac gactggttgg ggcggatgtt tcaggacatg agcgcgaaat acggaatctc
     1141 gatccacccg cgcaaggtca attcctcctc gctgtccttc atcatgccga aggacggcaa
     1201 gcgggcgatc gtgcgctgtc gcgacgacga gcatatccac cccttcccgc ttttgaacct
     1261 cggcggctgt cgcgcgctgc atatcgacgg ccaccagccg gacgccgcca tccactatgc
     1321 caagctgtgc cgcgaagacg gcatcttgac ttcgctcgac ggcggcggcc tacggaccaa
     1381 cacccacgaa ctcctggaat tcatcgacgt cgccatcgtc gccgaacggc tgtgcgagca
     1441 gatggacctg acgccggaaa agatgctgga ttatctcaag agccggggct gccgggtcgg
     1501 cggcgtgacc cagggcgaaa gaggcctgct ctggtacgac gaagcggggg cggttcgtac
     1561 catgcccgcg ctgccgatct cgcacgagcg cgtgctcgat accaacggtg ccggtgatgt
     1621 atttcacggc gcttacgtgt tttcgtatct caacaatccc ggcaaaagct ggcacgacca
     1681 tttcgaattt gcgcgcgcgg cctcgacata caagatccaa cgcctcggca acgaggccgg
     1741 attgccgacg cttgccgaca tcgaaaacgt caagcaagag tttcagatcg ggcgt
//
LOCUS       DASWGM010000757         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_625911,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000757 DASWGM010000000
VERSION     DASWGM010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625911"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGA15_20950"
     CDS             <1..373
                     /locus_tag="VGA15_20950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX9590201.1"
                     /translation="DSDLGYLLAPMNRGIRLTTGVEFARRDRAPTYIQIERALPRAHV
                     LFPLGDAVDAKPWMGARPCLPDMLPVIGKAPRHAGLWFDFGHQHHGLTLGPATGRLLA
                     EMMTGEAPFADPTPFAVERFG"
     gene            475..729
                     /locus_tag="VGA15_20955"
     CDS             475..729
                     /locus_tag="VGA15_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HEX9590202.1"
                     /translation="MKVNVEIDCTPLEARQFFGLPDLQPMQTAVMDKLQQQMLANIEK
                     VSPEALMQSWFTFDPKIAERFGDMFVTMAGLGGARSNDKK"
     gene            732..1529
                     /locus_tag="VGA15_20960"
     CDS             732..1529
                     /locus_tag="VGA15_20960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9590203.1"
                     /translation="MPIDRSEPAPPGSLRPPGLGLLLAEVRGIFEFNASLLLSPLLMR
                     APKGDGHPVLTLPGFLASDLSMAPMRRYLKELGYDTYAWKMGRNVGGVSRMRAALRER
                     LAEIYNATGRKVSVVGWSLGGVYARDLALQAPGMVRYVVTLGSPFANDVRATNATRLY
                     EALSGEAVEDNAALRAAISGDLPVPTTSIYSRADGIVNWRTCILRPSDTAENIEVHLA
                     SHVGLGVNAAALWAVADRLAQAEGEFSQFDRSGPFAIAYAPPKNAQS"
     gene            1609..>2525
                     /locus_tag="VGA15_20965"
     CDS             1609..>2525
                     /locus_tag="VGA15_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX9590204.1"
                     /translation="MSDAKKLSSMDASFLYLETPEMPMHVGSMAIFRLPEDYKGDFFE
                     DFKAMIASRLHVAPILKARLEKAPLDIDHPSWVEDDQFDIDRHIFRGSLPAPHDRATL
                     ERIVGWMHAKLLNRARPLWEFYVFEGMKDNEIGLYSKMHHACIDGGAGAALTNMIYDV
                     TPVPRVVEPATAKAKVGQEPRDIAANLVDSYQQLWTQSFDVKAAAKGIDLPRSGKSDL
                     GSILFDNAMFQIESAVKFAGSLPAMLKSVSDVVGKISDPKSRDSIASMVSPSTILNKS
                     ISSERSFAGVSISLSRAKALAKQAGGKLN"
BASE COUNT          470 a          768 c          819 g          468 t
ORIGIN      
        1 cgattcggat ctcggctatc tgctggcgcc gatgaaccgc ggcatccgtc tcaccaccgg
       61 cgtcgaattt gcccgccgcg atcgggctcc gacttacatc cagatcgagc gggccttgcc
      121 gcgggcgcac gtgctgttcc ctctgggaga cgccgtcgat gcaaagccct ggatgggcgc
      181 gcggccgtgt ttgccggata tgctgccggt gatcgggaag gcgcctcgcc atgccgggct
      241 gtggttcgat ttcggacacc agcaccatgg cctgacgctg ggccccgcca ccggccgtct
      301 gctggcggag atgatgaccg gcgaggcgcc gtttgccgac cccaccccct ttgccgtgga
      361 gcgtttcggc tgaccgtttg aggctcggga tacctatccc ttcaggcagc ttgcgccgcg
      421 ttgcctatcg tgacgatact gtgacgagcg ggcaaaatgc gaccaggagc ggacatgaag
      481 gtaaacgttg aaatagattg cacgcctctc gaggcccggc agtttttcgg acttccggat
      541 ttgcagccga tgcagacggc ggtgatggac aaactccagc agcagatgtt ggccaacatc
      601 gaaaaggttt caccggaagc gctgatgcag agctggttca cgttcgatcc gaagatcgcg
      661 gagcggttcg gggacatgtt cgtgaccatg gcgggcctcg gcggtgcgcg cagcaatgac
      721 aagaaatagc gatgccgatt gaccggagcg aaccggcgcc gccgggcagt cttcgtccac
      781 cggggctcgg cctgctgctg gccgaggtga gggggatatt cgaattcaac gccagcctgt
      841 tgctttcgcc gctcttgatg cgcgcgccga agggcgacgg tcatccggtg ctgacgttgc
      901 cgggtttcct cgccagcgat ctgtcgatgg cgccgatgcg aagatatctg aaggaactcg
      961 gatacgatac ctacgcctgg aaaatgggtc gcaacgtcgg cggcgtttcg cggatgcggg
     1021 ccgcgctgcg ggagcggctc gccgaaattt acaatgccac cggccgcaag gtcagtgtcg
     1081 tcggctggag cctcggcggc gtctatgcgc gcgatctggc gctgcaggca cctggcatgg
     1141 tgcggtatgt cgtgacgctg ggaagcccgt tcgccaacga cgtcagagcg accaacgcga
     1201 cgcggctcta cgaagcgctg tcgggcgaag cggttgagga caatgcggcg cttcgagcgg
     1261 cgatatcagg cgatctgccg gttccgacca cctcgatcta ttcgcgcgcc gacggcatcg
     1321 tgaactggcg gacctgcatc ctgcgtcctt ccgataccgc tgaaaacatc gaggttcacc
     1381 ttgccagtca cgtgggcttg ggggtcaacg ccgccgcctt gtgggcggtg gccgaccggt
     1441 tggcccaggc cgagggcgaa ttcagccaat ttgaccgatc ggggccgttt gccattgcat
     1501 atgcgccccc gaaaaatgca caatcctgac cgtgtcctga acttcagggc ggtctgaacg
     1561 ccagaagcgc caccaaaggc gcccgcgtga ttgtgccggg agggaactat gagtgacgcc
     1621 aaaaagctgt cctcgatgga cgcgtcgttt ctgtatctgg aaacgccgga aatgccgatg
     1681 catgtcggga gcatggcgat cttccgcctg cccgaggact acaagggcga cttcttcgag
     1741 gacttcaagg cgatgatcgc ctcgcggttg catgtggcgc cgatcctcaa agcgcggctg
     1801 gagaaggcgc cgctcgacat cgatcatcct tcctgggtcg aggacgacca gttcgacatc
     1861 gaccgccata tcttccgcgg cagccttccc gcgccgcacg accgcgcgac gctggagcgg
     1921 atcgtcggct ggatgcatgc caagctgctc aatcgcgccc gcccgctgtg ggagttctat
     1981 gtcttcgagg gcatgaagga caacgagatc gggctctatt ccaagatgca tcacgcctgc
     2041 atcgacggcg gcgccggcgc ggcgctgacc aacatgatct acgatgtcac gccggttccg
     2101 cgggtggtag aaccagcgac ggcgaaggcg aaagtcgggc aggagccgcg cgacatcgcc
     2161 gcgaatctcg tcgactccta ccagcagctt tggacccagt cgttcgatgt caaagccgcc
     2221 gccaagggca tcgacctgcc gcgctcggga aaaagcgatc tcggatcgat cctgttcgac
     2281 aacgcgatgt tccagatcga atccgcggtg aagtttgccg gcagccttcc cgccatgctc
     2341 aaaagcgttt cggatgtggt tggcaagata tccgatccga aatcgcgcga cagcatcgcc
     2401 agcatggtgt cgccgtcgac gatcctgaac aaatcgattt catcggagcg cagctttgcc
     2461 ggggtatcga tctcgctgag ccgggcgaaa gcgctggcca agcaggccgg cggcaagctg
     2521 aacga
//
LOCUS       DASWGM010000758         3106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1178918,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000758 DASWGM010000000
VERSION     DASWGM010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178918"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VGA15_20970"
     CDS             <1..1020
                     /locus_tag="VGA15_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9590205.1"
                     /translation="HNTGGLGPRASYHVQFDGSVPGLLVGAAVLFNGIRVGEVTDLGL
                     APDNPRRVNATISVSSTTPVRSDTKVGLEFQGLTGVPVIALEGGTQLVNSGEVPTLIA
                     EPGAGQSMTQAARDALRKVDSVLAENAEPLKNTIANLQVFSEGLARNTGKLDSIVGGL
                     EKMTGGGAPAQKITYDLRAAQNLGPPSKTIKGQLAIPEPTAVAMLETQRMLFSPAKDV
                     PGFADFLWADAIPKLLQARLIEGFENYDIAHAPLRNADVGQADFQLLIDVRRFRVAVD
                     SEPTAEIGLSARIVDKNGKVIASRLFEDRQKFDKVEPTAAAAAFDDAFGRIAKDMIAW
                     TVQAL"
     gene            complement(1058..1375)
                     /locus_tag="VGA15_20975"
     CDS             complement(1058..1375)
                     /locus_tag="VGA15_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9590206.1"
                     /translation="MLRFASRQTLFMLMLATPALAASPAEQRGKTFALTNCARCHSID
                     RVTKSPLKIAPPFRTLHLRYPVETLGEALAEGIETGHPTMPAFQLDPDQINDLLSYLK
                     SLE"
     gene            1548..2261
                     /locus_tag="VGA15_20980"
     CDS             1548..2261
                     /locus_tag="VGA15_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9590207.1"
                     /translation="MKISLVAGEPNGQPCTDCSVRQFSVCAAFDKAELRELEHLGRHV
                     HFRTCERVFEQEELTTSFYNLLEGVMRLYKLLPDGRRQIVGFGLPGDFLGMGTSARHS
                     LSADAIGPVVVCRFARTSFARFIEDKPHLLRRINEFAVRELSQARDHMVLLGRRSAEE
                     KVASFLIGWRDRLAQLSGPMKTVPLPMSRQDIADYLGLTIETVSRTFTKLERDHVIDI
                     LPGSVCLLDPARAEALAAA"
     gene            2799..>3106
                     /locus_tag="VGA15_20985"
     CDS             2799..>3106
                     /locus_tag="VGA15_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9590208.1"
                     /translation="MRLLVSLVPALALLASSHAIAQTAYPDRPIKMIVPLAAASAVDV
                     AARIVTQKMADNMGQQFVIMNQPGASGLIGAEAVARAEPDGYTIGGFNDSIMTMVPNL
                     "
BASE COUNT          591 a          881 c         1022 g          612 t
ORIGIN      
        1 cacaataccg gtggcctcgg tccccgcgcc agctatcacg tccagttcga cggttcggtg
       61 cccgggcttc tggtcggcgc cgccgtgctg ttcaacggaa tccgcgttgg cgaagtgacc
      121 gatctcgggc tggcgccgga caacccgcgc cgcgtcaatg cgacgatctc ggtatcctcg
      181 acgacgccgg tgcgatccga caccaaggtt ggcctggagt tccagggctt gaccggcgtt
      241 cccgtgattg cgctggaggg cggcacgcag cttgtgaatt caggtgaggt accaacattg
      301 attgccgaac ccggggcggg ccagagcatg acccaggccg cccgcgatgc gttgcggaaa
      361 gtggattccg tgctggccga aaatgcggag ccgctgaaga acacgattgc caatttgcag
      421 gttttctccg agggcctggc gcgaaacaca ggcaagctcg acagcatcgt cgggggactt
      481 gaaaagatga ccggcggcgg tgcgccggcg caaaaaatca cttacgactt gcgtgccgcg
      541 caaaatctgg gaccgccgag caagaccatc aagggacaat tggcgatacc ggagccgacc
      601 gcggttgcca tgctcgagac ccagcgaatg ctgttctcgc cggcaaaaga cgttccggga
      661 tttgcggatt ttctctgggc cgacgccatt ccgaaactgc tgcaggccag gttgatcgag
      721 ggctttgaga actacgatat tgctcacgcg ccgttgcgca acgcggatgt cggccaagcc
      781 gatttccagc ttctgatcga tgtcaggcgt tttcgggtag cggtggattc ggagccgacc
      841 gccgagatcg gactgtcggc gcggattgtc gacaagaatg gcaaggtgat cgcgtcgcgc
      901 ctgtttgagg accgccagaa attcgacaag gtcgagccga cggcagccgc cgccgcgttc
      961 gacgacgcgt tcggccggat cgcgaaggat atgatcgcct ggacggtgca ggcgctgtag
     1021 cagccgccgc gttcgcgcgg acgttgccgc gcgaaatcta ttcgaggctc ttcagatacg
     1081 acagcaggtc attgatctgg tcgggatcga gctggaacgc cggcatcgtc ggatgaccgg
     1141 tttcgatgcc ttcggcgaga gcttcgccga gggtttcgac cgggtaacgc agatgcaggg
     1201 ttcgaaacgg aggcgcgatc ttgagcgggc tttttgtcac gcgatcgatg gagtgacacc
     1261 gcgcgcaatt ggttagcgca aaggtcttgc cgcgctgttc ggcgggcgag gcggcgagcg
     1321 ccggtgttgc cagcatcagc atgaacaggg tttggcgcga agcaaatcgc agcattgcgg
     1381 accgttctcc ttagattgcg gctacagcgg acaagccttg atagtgcggc gaggccggtc
     1441 gtgcttgatc tgcatcaacg cgaaaatggg cgatccgcta agatgcagtt gacccgcggg
     1501 ctcagcgcac agtgtggttg cagcccagcc ccatcctgga gtggtgcatg aagatttccc
     1561 tggttgccgg cgagccaaat gggcaaccct gtacggattg ctcggtgcgc cagttcagtg
     1621 tctgcgcggc gttcgataag gctgaactgc gggaattgga gcatttgggc cgccatgtcc
     1681 atttccgaac ctgcgagagg gtgttcgagc aagaggagct gacgacgtca ttttacaatc
     1741 tgctcgaggg cgtcatgcgg ctttacaagc tgctgcccga cggccggcgg cagattgtgg
     1801 gatttgggtt gcccggcgat tttctcggca tgggaacctc cgcgcgtcac agcttatccg
     1861 ccgacgcgat cggacccgtg gtcgtttgcc ggttcgccag aacctccttc gcccgtttta
     1921 tcgaggataa accgcatctc ttgcgccgga tcaacgaatt cgcggtccgc gaattgagcc
     1981 aggcccggga tcacatggtt ctgcttggac gccgttcggc ggaggaaaaa gtcgcgagtt
     2041 ttctgatcgg ctggcgcgat cgattggctc agctgtcggg tccaatgaag acggtgccgc
     2101 tgccgatgag ccgccaggac atcgcggatt acctcggcct gaccattgaa acggtcagcc
     2161 gtacgttcac caagctcgaa cgcgatcatg tgatcgatat cctgccggga agtgtttgcc
     2221 tgctggatcc cgcgcgcgcc gaggcgctgg cagcggcctg agcccagagc aaacaacgcg
     2281 ctccttaacg ccgcttttga gatttgatcc ggatcaagga ccggcatttc tcgtcgcgga
     2341 taaatgcctc cagttagatc agggaggtca ttatgccgtt tgatgcgatg ttagtgagcg
     2401 tagccgtggc aatcatgttc ttggcgtttg cgggcgtgct gctgtggggc gactttcaga
     2461 cgcggccaga tcggctgaag gccaactctc aagctcaaaa gcgtcgaagc ttttgatgca
     2521 cgtcccgctg acgcagggga ttggacggag aacgcgccat gggtcgggcg ggttgggtga
     2581 cggatgcctg aggttcgggc ccggttgccg cactgcagca tcgggcagtt cggagcagcc
     2641 aagcttccct catcccgaac aggcgcgccg cgttgccgcc cagaatttcg accttccgct
     2701 cgcgcagatt gccaaggtgg cggcgagaga gcaatctttc ctgcaaggat tacggccgga
     2761 taaaatgaaa acgtgatcca atacgggagg gattgggtat gcgcttgctt gtttcgctgg
     2821 ttccggcgct cgcgctcctt gcaagctccc atgcgatcgc gcagaccgcc tatccagacc
     2881 ggccgatcaa gatgatcgtg cccctggcgg cggcgagcgc ggtggatgtc gcagcgcgga
     2941 tcgtgacgca gaagatggcc gataacatgg gccagcaatt cgtgatcatg aaccagccgg
     3001 gcgcgtccgg gctgatcggc gccgaagcgg tcgcgagggc cgagccggat ggctacacga
     3061 tcggcgggtt caacgacagc atcatgacca tggtgccaaa cctgca
//
LOCUS       DASWGM010000759         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2460527,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000759 DASWGM010000000
VERSION     DASWGM010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460527"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..365
                     /gene="grxD"
                     /locus_tag="VGA15_20990"
     CDS             27..365
                     /gene="grxD"
                     /locus_tag="VGA15_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX9590209.1"
                     /translation="MSIEQFIDNEVKSNDVVLFMKGTPQFPQCGFSGQVVQILDHVGV
                     GYKGLNVLESADLRNGIKTYSNWPTIPQLYVKGEFVGGCDIIREMFQAGELQQLLADK
                     GVTVSAAASA"
     gene            362..916
                     /locus_tag="VGA15_20995"
     CDS             362..916
                     /locus_tag="VGA15_20995"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX9590210.1"
                     /translation="MSPSARLIGTARLEVIVADITTLRVDAIVNAANTSLLGGGGVDG
                     AIHRAAGPELLAECRALGGCETGDAKITSGYRLPARHVIHAVGPVWHGGSQSEDEALA
                     SCYRRAIELGQAHGLASMAFPAISTGVYRFPADRAAGIAVSATADALVAAPVVTRIIF
                     CCFSDDSARLHAQAMAQLGSPCAD"
     gene            958..2241
                     /locus_tag="VGA15_21000"
     CDS             958..2241
                     /locus_tag="VGA15_21000"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9590211.1"
                     /translation="MNSGVVLRALTCGALLTLAAPSAIAEGTFDIPAGAHFNPQKLDT
                     IGEFFRNEVTTGKISGAILLIQQHGKPVYHESFGVQDVVSKAPITDQTIFRLFSMTKA
                     ITSVVAVQLIDEGKLALEDPVAKYIPSFANVKVGVEKKADDGTKTLELVPPVRPPTIL
                     DLMRHTSGITYGFYGDSLVRKTYAAANLYAGDFDLAEFAERIAKLPLHDQPGSLWQYG
                     HSTDVLARIMEIVSGKSLIEVERERLLDPLGMRDTGFFVTDPAKQKLIAQPVPNDSDF
                     RVGRENRPDVPKKWESASGGMVSTMSDFARFAQMLLDGGKFEGKTYLSPKAFELMTTD
                     HVGPGSGVGRDYFYFPGDGFGFGLGLAVRTDPGNAKPPPPGSLGELKWDGASGCYFVI
                     DRKQDMFFVLLEQTPTERQRIQRTAKKLIYEAMEN"
     gene            2222..3079
                     /locus_tag="VGA15_21005"
                     /pseudo
     CDS             2222..3079
                     /locus_tag="VGA15_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
BASE COUNT          619 a          948 c         1025 g          639 t
ORIGIN      
        1 gggccgtgcc gcaaaaggac gcgaatatga gcatcgagca attcatcgac aacgaagtga
       61 agtcgaacga cgtcgtgctg ttcatgaagg ggacgccgca gtttccgcag tgcggttttt
      121 ccggccaggt ggtgcagatc ctcgatcacg tcggggtcgg ctacaagggg ctgaatgtcc
      181 tggagtccgc cgatctgcgc aacggcatca agacctattc caactggccg accattccgc
      241 agctctacgt caagggcgaa ttcgtcggcg gctgcgatat catccgcgag atgttccagg
      301 ccggcgagct gcaacagctg cttgccgaca agggcgtcac ggtcagcgcg gctgcttccg
      361 cctgagcccg agtgcgcgcc tgatcggaac ggcgcggctg gaagtcatcg tcgccgacat
      421 caccacgctc cgcgttgacg cgatcgtgaa cgcagcgaat acgtcgctgc tcggcggcgg
      481 cggcgtcgat ggtgcaatcc atcgcgccgc gggacctgag cttctcgccg aatgccgcgc
      541 gctcggcggt tgcgaaaccg gcgacgccaa aatcaccagc ggttatcggt tgccggcgcg
      601 gcatgtgatt catgccgtcg gcccggtctg gcatggcggg agccaaagcg aggacgaggc
      661 tttggcgtca tgctatcgcc gcgccatcga gctcggccag gcccatggcc tcgcttcgat
      721 ggcctttccc gcgatctcca ccggcgtcta tcgtttcccc gccgaccgtg ccgcgggaat
      781 cgcggtctcg gcgacggcag atgcgctcgt cgcggcgccc gtggttaccc gcatcatctt
      841 ctgctgcttc tccgacgaca gcgcccggct gcatgcgcag gcgatggcgc aattgggcag
      901 cccttgtgcc gattgagccg gccgctacac tcccggggga ttttggggga gggaacaatg
      961 aattcaggcg ttgtgttgcg cgcgctgacg tgtggagcgc tgctgacgtt ggcagcgccg
     1021 tcggcgatcg cggaaggtac tttcgatatt cccgcgggcg cgcatttcaa cccgcaaaag
     1081 cttgatacga ttggcgagtt cttccgcaac gaagttacga ccggcaaaat ctcgggcgcg
     1141 attctgctca tccagcagca cggcaagccg gtctatcacg aatccttcgg cgtgcaggac
     1201 gttgtctcca aggcgccgat cactgaccaa accatctttc gtctgttctc gatgaccaaa
     1261 gccatcacca gcgtggtcgc ggtgcagttg atcgatgagg gcaagctcgc gctggaggat
     1321 cccgtcgcga agtacattcc ctccttcgcg aatgtgaagg ttggcgtcga gaagaaagcc
     1381 gacgatggta caaagacgct cgaattggtg ccgccggtcc ggccgccgac cattctcgac
     1441 ctgatgcgtc acacgtctgg aatcacctac ggcttttatg gcgatagcct ggtccgcaaa
     1501 acttatgcgg ccgcaaacct ctacgcgggc gatttcgacc tcgccgagtt cgccgagcgg
     1561 atcgcgaagc tgccgctgca cgatcagccg gggtcgctct ggcagtacgg ccattccacc
     1621 gacgttctgg cacggatcat ggaaattgtg tctgggaaat ccctgatcga ggttgagagg
     1681 gagaggctgc tcgatccgct cggcatgagg gacaccggtt tctttgtcac ggatccggcg
     1741 aagcagaagc tgatcgccca gccggtgcca aacgacagcg attttcgggt cggccgcgag
     1801 aacaggcccg atgttcccaa gaagtgggaa tccgccagcg gcggtatggt ttcgactatg
     1861 tcggacttcg cgcgctttgc gcagatgctg cttgatgggg gcaagttcga aggtaagacc
     1921 tatctgagcc ctaaggcctt cgaactgatg acgacagatc acgtaggccc gggttccggc
     1981 gtcgggcgcg actatttcta tttccccggc gatggcttcg gcttcgggct cggtctcgcg
     2041 gttcgcaccg atccggggaa tgcaaagccg ccgccgccgg gctcgctggg cgagctgaaa
     2101 tgggacggcg ccagcggctg ctatttcgtg atcgaccgca agcaggacat gtttttcgtg
     2161 ttgctggagc agacacccac ggagcgtcag cgcatccagc ggacggcgaa gaagctgatc
     2221 tatgaagcaa tggagaactg acggtcttgc gagccggcgc agcgtcgcgg cggtgctcgc
     2281 gcttctggtt ctggtagttg cggtcggacg gccgggcgcg ggtaacgcag aatcaccacc
     2341 atcagtgccg gcaggctttt cgcgcgcgaa actgcagcgc gtcggcgatt atatccgtaa
     2401 cgagattgcg accggcaaga tccccggcgc cgtcgttctg attcagcagc acggtcagcc
     2461 ggtgtatttc gaaagtttcg gcgtgcgcga cgtcgaaagc aggctgccga tgacgccaga
     2521 caccatcttt cggctgtatt cgatgtcgaa gccgatcacc agcgtcgcag tgatgacgct
     2581 ggtagaggag ggcaagctca ggcttgatga tctcgtttca aagtatcttc cggcattcgc
     2641 ggacgtgaag gtcggcgtcc aaaagtctga tgaaaacggc aagccagcgc ttgctctgga
     2701 accggtcaac cggccgatca ccatcgaaga cctgatgcgc catacgtcgg gccttactta
     2761 cggcttttac ggcgatagcg aggcacgaag gctctatgcc gaggctgatc tcttcaatcg
     2821 agacaccgac aatgccgaat tcgtcgagcg gatcgcgaaa ttgccgctcg ccgagcagcc
     2881 gggaacgcta tgggattacg gccactcgac cgatgtgctc ggtcgcgtca ttgaggttgt
     2941 atccggaaaa tcgctgttcg ggtttgagaa ggaacgcctg ctcgatccct tgggaatgac
     3001 cgagacggcg ttctacgtcg ccgaccaagc gaagcggccg cgcatcgctg agccgatgcc
     3061 ggatgatcgc tcagtcagcg cggtcacgag ccttagcact acagttgctt tttatgcagg
     3121 tttctgaatc catgggcaac catgattcga agatcgtgga cgcggccagc gtcgattgaa
     3181 gagacgcttg cgctgtgggc ggcgtcgctt cgtgatatca agaaacggat a
//
LOCUS       DASWGM010000760         7281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1587759,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000760 DASWGM010000000
VERSION     DASWGM010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587759"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..3112
                     /locus_tag="VGA15_21010"
     CDS             11..3112
                     /locus_tag="VGA15_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEX9590212.1"
                     /translation="MSQPASQAAPSAADVAKDASASLVAPPRTISDISAILDQQKPDP
                     AVVARLTETADAAVPAGLKAPELAEFYYKRSQARTLLGRADAIDDAELAVSNAQGGGD
                     YYKNVASRYEALLMRWLHDAGQHKRANVLIAKQVAAFANQGKGKLFGLNYVLALGYLR
                     NGDTNTAESYVARNRVLLTEAQRWPVFPIYGMAWQAAVEDGNARIEEARGRLPQAEAA
                     FHRAAILYTSSLKTISQWESKPAEGELERHADWALAFEGRAKVKQGRVGEGEADVRRA
                     LLSRLSKSGKFHADTAGVLAVLVYVIQEQGRYQEAEQLQRQVIEIYRGLGYGAESAQM
                     VIAHLFLAQILNLERLYDAASKLYDQIDVWTAKWEPSRREPVSGGLARVGVMLSQGSN
                     DNALEIAQRTFERERQKSGDKSFNTAISRGFYAIALARKSRTSEALQAYKDSLPVLLS
                     TSGGGDDDSGATAAAREGRIRFVIEGYLRLLSRNPSIVPAGVVEETFGYADVLRGQSV
                     QRALQASSVRSATKDPVLAQLMRASQDGEKRIGAAVATLNNLLSLPTSERDDKALTDT
                     QQEIARLQATHAQILKDLAKKFPDYGNLVNPAAPSPVDLQKQLTADEALLSFYFGRFD
                     SFVWILRKDQPVQFVRIETTLGDMNAKVAKLREALEPQAAMISDIPAFDLDLAHQLFS
                     MLLKPTEASWRPAKSLIIITNGALGLLPLSLLPTMPSKIVPDDDPLFSSYRSVPWLAR
                     THAVTLVPSISALLTLRKLPQGTASRQELIGFGDPLFSQQQAEEARKESAPVQVADAS
                     NVTRGMRLKRRNSPKLDGVDSAQLAQLPRLPDTAEELKSIALALQADPAKVLNLGKDA
                     NEKTVKSTDLSKFKIIAFATHGLVPGELDGLTQPALALSAPAVADSDGDGLLTMEEIL
                     GLKLDADWVVLSACNTGAGVVAGAEAASGLGRAFFYAGTRALLVTNWSVHSQSARELI
                     TDLFKRQADDPKITRGEALRQAMMALVDGPGYVGADGKTEFAYAHPLFWAPYSIIGDG
                     GGR"
     gene            3143..3595
                     /locus_tag="VGA15_21015"
     CDS             3143..3595
                     /locus_tag="VGA15_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590213.1"
                     /translation="MRSWYVGLLIAFAAVVSSAAIPAHAAQVLITEEEAKLPPPKGAV
                     VADRRGVTRAPKVEYIEGSKEIHSPRRLQLKFESFGGAKIDPDSVKVTYLRTPNVDLT
                     SRVKSFVQATGIDIPDVELPVGDHMVRVDIKDSDGRVGSTSFVLRVAP"
     gene            3900..4577
                     /locus_tag="VGA15_21020"
     CDS             3900..4577
                     /locus_tag="VGA15_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590214.1"
                     /translation="MLEVQEIVSELDQLRRELDHVSAKLDWTRHPFRAALTYVAVYLV
                     IPTALLVAAHVLVTIVLDVTPLYLRLASVVIPLPFGLAIYAREKVGVRGAILVGALTA
                     TIAVVCMLTVTGLNDKVSIVPASWIEWREAIEYAVSIALAFVTGNIIGFLIFQALPKI
                     MTRGGKPSAVAYNVARALGQHVGEEQLRRRARIIQDLLLTLGPLMGIVVTASGSIYAG
                     LKGIFGW"
     gene            4803..7004
                     /locus_tag="VGA15_21025"
     CDS             4803..7004
                     /locus_tag="VGA15_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9590215.1"
                     /translation="MNRRFRPLTWFSFAMLVAFSNDAVALARHKQVQHARKADAAAGG
                     HHRNAALKKGKHAAHEAAARHKPTPPVEKPPQAAAASPLTGDLAAVKQAFDLVRKAKT
                     GEATAIEETIGDPAAQKLVEWLILRHPDGAANFGRYAAFIADNPSWPSTSPLRRRAEA
                     RLWQERSDAATVRSFTGGQPATARGRFALARVLLAEGDRDGAQNQVREAWRSEELSER
                     VEADALDVFRDLLTRDDYRARMDKRIGAKDFSGAMRAAHRLGSDDVAIVKACAAVSGG
                     ADKALELLDDVQTEARQDLGYILCRIHWMMGHNKLADATRLMLAAPSKSMPLQDTDEW
                     WRERRVLARKLLDLHEFHGAYQVVRNAALPANENYRAEFHFLPGWIALRYLNDPATAR
                     EHFAHIDDGSVNPIVLARANYWRGRAAEAAGENDKMRAGYEAAARHGTAYYGQLARAK
                     LGLEKIELRPPLQPDPSNGPALSDELVRAADMLYALGERDVVLNFVTDFAEQSADVAA
                     LAALGELTARRNDARAMLQIGKTALARGLALDLYAFPTIGVPQHSPVGPEIDRSVVYS
                     VARTESAFDQRDASPAKAVGLMQVTPAAGRDTAKRFGVTYDWDKMVSDPVYNTQMGAA
                     ELGALLREYRGCHIMTFAGYNAGRGRVQQWVKQYGDPRDPNVDAVDWVERIPFAETRN
                     YVQRVMENLQVYRVRFDTGTRVMSKLDQHRETTQEANSASLSSMQPAFAEW"
     gene            complement(7122..>7281)
                     /locus_tag="VGA15_21030"
     CDS             complement(7122..>7281)
                     /locus_tag="VGA15_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEX9590216.1"
                     /translation="VRLRDEANVPMVYVSHDAAEMRQLATQIVMLRHGQVTAFGGVKV
                     LSAPVSPA"
BASE COUNT         1402 a         2174 c         2305 g         1400 t
ORIGIN      
        1 ggcagagaaa ctgtcgcagc cggcttctca agccgcgccc agcgccgcgg acgtcgcgaa
       61 ggatgcctcg gcaagtcttg tcgctccgcc gcgaaccatc tccgacatca gcgccattct
      121 cgatcaacaa aagccggatc cggccgtcgt cgccaggtta accgagacgg cggacgccgc
      181 tgtcccggcg ggcctgaagg ctccggagct tgcggagttc tattacaagc gctcgcaggc
      241 acggacgctg ctcggtcgtg ccgacgcgat tgacgatgcg gagcttgcgg tgagcaacgc
      301 acaagggggc ggcgactatt ataaaaatgt agcgagccgt tacgaggcac tgctgatgcg
      361 ctggctgcac gatgccgggc aacacaagcg tgccaacgtg ctgattgcca agcaggttgc
      421 ggcgtttgcc aaccagggaa agggaaagct gttcggattg aattacgtgc tcgcgctcgg
      481 ttatctcaga aacggcgata ccaacaccgc tgaatcttat gtcgcgcgca atcgcgtact
      541 gctgacggaa gcccagcggt ggccggtgtt cccgatttac gggatggcct ggcaagccgc
      601 cgtcgaggac ggcaacgcgc ggatcgagga ggcgcgcggt cggcttcccc aggcggaggc
      661 cgcatttcat agggcagcga ttttatacac gagctcgctg aagacgattt cgcaatggga
      721 aagcaagccc gccgaaggcg aactggaaag gcacgccgac tgggctcttg cctttgaagg
      781 gcgcgccaag gtgaaacaag gcagggttgg tgaaggcgag gccgacgtca ggcgcgcgct
      841 gctgagccgc ttgagcaaat ccggcaaatt tcatgcggat acggccggcg ttctcgcggt
      901 tctcgtctac gtgattcagg agcaaggccg ctaccaggag gccgagcagc tgcaacgcca
      961 ggttatcgaa atttatcgag ggctcggata cggcgctgaa tccgcgcaga tggtcattgc
     1021 gcacctgttt ctggcgcaaa tcctgaatct cgagcggctg tatgatgccg cctccaaact
     1081 ctacgaccag atcgacgttt ggaccgccaa gtgggagccc tcgcggcgtg aacccgtcag
     1141 cggcggtttg gcgcgagtcg gcgtgatgct ctcgcaaggt agtaacgaca atgctctcga
     1201 gattgctcag cgtacgttcg agcgcgaacg tcagaaatcg ggagacaaaa gcttcaatac
     1261 ggccatatcg cgcggatttt atgccatagc ccttgcgcgg aagagcagga catccgaagc
     1321 attgcaggct tacaaggact ccctgccggt cctgctatcg acctcgggtg gcggcgatga
     1381 cgatagcggc gcaaccgccg cggcgcgcga aggtcggatc cggtttgtga tcgagggtta
     1441 cttgcggctt ctcagccgga acccgtcgat cgtgccggcc ggtgtcgtcg aggaaacctt
     1501 cggttatgcc gacgtgctgc gtggacaatc ggtccagcgc gcgctgcagg cgtcatccgt
     1561 gcgttcagcc accaaagatc cggtgctggc gcaattgatg cgcgcatcgc aggatggcga
     1621 aaagcggatc ggcgccgcgg tggcgacgct caacaatctg ctgagcctgc cgacctccga
     1681 gcgtgacgac aaggcactta ccgacacgca acaggaaatt gctcgcctgc aggcaactca
     1741 cgcgcaaata ttaaaggacc tcgccaaaaa attccccgat tacggaaacc tcgtcaatcc
     1801 ggcggcgccg agcccggttg atctgcaaaa acagctcacc gccgatgagg cattgctttc
     1861 gttttacttc ggccggtttg acagcttcgt atggattctg cggaaggacc agcccgtcca
     1921 gttcgtgcgc atcgaaacca cgctcgggga catgaacgcc aaggtggcca agctgcgcga
     1981 ggcgctcgag ccccaggctg caatgatctc cgatattccg gcattcgatt tggacctggc
     2041 ccaccagctg ttttcgatgt tgctgaagcc gacagaggca agctggagac cggcaaaaag
     2101 cttgatcatc attaccaacg gcgcgctcgg tctgctgccg ctgtcgttgc tgcccaccat
     2161 gccctcgaag atcgttcccg acgatgaccc gctgttctcc agctatcgga gcgttccgtg
     2221 gcttgcgcgt acccatgcgg taacgctggt gccttcgata tccgcgctgc tcacgctgcg
     2281 aaaattaccc cagggcacag cttcgcgtca ggagctgatc ggtttcggcg atccgttgtt
     2341 cagtcagcag caggcggagg aagccaggaa agaaagcgcg ccggtacaag tcgcagacgc
     2401 gtccaatgtc acgcgcggaa tgcggttgaa gcggcgcaat agcccgaaac tggatggcgt
     2461 cgatagcgcc cagctggcac agttgccgcg gcttccggat accgcggaag agctgaaatc
     2521 gatcgcgctc gcactgcaag ccgatccggc caaggtgctt aatctcggca aggacgccaa
     2581 cgagaaaacc gtcaagtcga cagacttgtc gaaattcaag atcatcgcgt ttgccactca
     2641 cggattggtg ccgggcgagc tcgacggcct gacgcagccg gcgctggcgc tctcggctcc
     2701 ggctgtggcc gacagcgacg gcgatggcct gcttacgatg gaggaaattc ttgggttgaa
     2761 gcttgacgcg gactgggtcg ttctgtcggc gtgtaatacc ggcgccggcg tggtcgctgg
     2821 cgccgaagcc gcatccggtt tgggccgtgc atttttctat gccggaactc gcgctttgct
     2881 ggtgacgaac tggtcggttc attcgcagtc tgcgcgcgag ctgataaccg atctgttcaa
     2941 gcggcaagct gacgatccca agatcacgcg cggcgaagca ttgcggcagg cgatgatggc
     3001 gttggttgat ggccccggct acgttggcgc agacggcaag acagagtttg cctatgcaca
     3061 cccgttattt tgggcgccgt attccatcat cggagacggc ggcgggcggt aatattgccg
     3121 gttcatgcag gggaagatcg tcgtgcgaag ttggtatgtt ggactattga ttgcatttgc
     3181 tgctgtcgtt tcgtctgcgg caattccggc tcacgcggcc caggttttga ttaccgagga
     3241 agaagccaag ctcccgccgc ccaagggcgc cgtggtggcc gaccggcggg gagtgacccg
     3301 ggctcccaag gtcgaatata tcgagggcag caaagagatt cattcgccga ggcgtctgca
     3361 gctgaagttc gaatcgttcg gaggcgcaaa gatcgatccg gactccgtca aggttaccta
     3421 tctcaggacc ccgaatgtcg atttgacgtc acgcgtcaaa tccttcgtgc aggccaccgg
     3481 catcgatatc ccggatgtcg agctgccggt cggcgatcac atggtcaggg tcgatatcaa
     3541 ggattcggat ggccgtgtcg gcagcacgag cttcgtctta cgggtcgctc cttaattgcc
     3601 atgttgatca tttgtccatt ctgcacacag gaaaatgcct cggttgcgtt ggtttgcagt
     3661 tcatgcgccc gcgacatcgc cgtgccggtc tcgctcatcg acgaacgaga cgatcttctt
     3721 cgaaagcgtc acgcggtgcg ggaggaattg ttggcagcca agcgtgaact tgcgagtctc
     3781 atgcgcggca agcaacgtcg accactctga tggagtgccc attctgcgct gaaatcatca
     3841 aggatgaggc catcgcctgc aagcactgct cgcgtgatct gcgagttgcc cgcccggtga
     3901 tgctggaagt tcaggaaatc gtttccgaac tcgaccagtt gcggagggaa ctcgatcatg
     3961 tcagtgccaa acttgattgg acccgccatc cttttcgcgc tgcgctgact tacgtggcgg
     4021 tctatctggt catacccacc gcgctcctgg tagcggcgca tgttcttgta accattgtat
     4081 tggatgttac gccgctttat ctccggttag cctcggtggt tattccgcta ccgttcggtc
     4141 tcgcgattta tgcgcgggag aaggtcggtg ttcgtggcgc catcctggtc ggcgcgctca
     4201 cggcaaccat cgccgtagtc tgcatgctca cggtgaccgg gttgaacgac aaggtatcga
     4261 tcgttccggc atcctggata gaatggcgcg aggcgattga atatgcggtg agcatagcgc
     4321 ttgctttcgt gacgggtaac attatcggct ttttgatctt ccaggcgctc ccgaagatca
     4381 tgacgcgtgg cggaaaaccg agcgctgtcg cctacaatgt ggcacgggcg cttggtcaac
     4441 acgtcggcga ggagcaactc cgccgccgag cgcgaattat tcaggatctg cttctcacct
     4501 tgggtccgct gatggggatc gtcgtgacgg cgagcggttc aatttatgcc ggtcttaaag
     4561 ggatattcgg ctggtgattt gccgccttcc gagaagcgat gtctcgcgca tgttggacgc
     4621 cgatggcaac cgctcggtca cccaggcgac gtgctcggtt caggctcacg ccacgttttc
     4681 ccccgtgtgt tgccgaaacg tgacagttgc gatgaggcgg tgtgatagcc ttcgtgcagt
     4741 tcgaaccgca cggatcgtgc gatctcatgg ttttcgccac ggggctcgcg ggggatcacg
     4801 gaatgaaccg acgctttcgg ccgctgactt ggtttagctt cgccatgctc gtcgcgtttt
     4861 cgaacgacgc ggtagcgctt gcccgccaca agcaagttca gcatgccagg aaggcggatg
     4921 cggcggccgg gggccatcac cgcaacgccg cgctcaagaa aggcaagcat gcggcgcatg
     4981 aggcggcggc gcggcataag ccgacgccgc ctgtcgaaaa accccctcag gctgcggcgg
     5041 cctccccact gaccggcgac ctcgccgcgg tcaagcaggc gttcgatctg gtgcgcaagg
     5101 ccaagactgg cgaagcgacg gctatcgaag aaaccatcgg cgatccggcg gcccaaaaac
     5161 tcgtcgaatg gctcatcctg cgccacccgg acggtgcagc gaatttcggc cgctacgccg
     5221 ctttcatcgc cgacaatccg agctggccga gcaccagccc gctgcgtcgg cgggcggagg
     5281 cgcgcttgtg gcaggagcgc agcgatgccg cgacggtgcg cagcttcacg ggcggtcagc
     5341 cggcgaccgc caggggccgg tttgcgctcg cgcgggtcct gcttgccgag ggcgaccgtg
     5401 acggcgccca aaaccaggtt cgcgaggcct ggcgttcgga ggagctgtcg gaacgcgttg
     5461 aagccgacgc gctcgatgtg ttccgcgatc tgctcacgcg cgacgactat cgggcacgca
     5521 tggacaagcg catcggcgcc aaggattttt caggcgcgat gcgcgccgcg catcggctcg
     5581 gaagcgacga tgtcgcgatc gtgaaggctt gcgcggccgt ctcgggcggc gctgacaagg
     5641 cgctcgagtt gctcgacgat gttcaaaccg aggcccgcca ggatttgggt tacattttgt
     5701 gccgcattca ttggatgatg ggtcacaaca agctcgccga cgcaacccgt ctgatgctgg
     5761 cagccccgtc caagtccatg ccgctgcagg acaccgacga atggtggcgc gagcggcggg
     5821 ttctcgcccg caagctactc gacttgcatg agttccatgg tgcttatcag gtcgtgcgca
     5881 acgccgcact cccggccaat gaaaattacc gggcggaatt ccattttctg cccggctgga
     5941 tcgcgctgcg ctatctcaac gatccagcaa cggcgcgtga acatttcgcc catatcgatg
     6001 atggctcggt taatccgatc gtgctcgcaa gggcgaacta ctggcgaggt cgcgccgccg
     6061 aggccgccgg cgagaacgac aagatgcgcg ccggctacga agctgccgcc cgccatggca
     6121 ccgcctatta cggacagttg gcgcgcgcaa aactcggcct tgaaaaaatt gaactgcggc
     6181 ccccgctgca gcccgatccg agtaacggcc ctgcgctttc ggacgaactg gtgcgcgccg
     6241 ccgacatgct ctatgcgctt ggcgagcgcg acgtggtcct gaatttcgtg acggatttcg
     6301 cggagcagag tgccgacgtc gctgcattgg ctgcgctcgg cgagttgacc gcgcggcgca
     6361 acgatgcgcg cgccatgctg cagatcggga agacggcgct cgcccgcggc ctggcgctgg
     6421 acctctacgc ctttcccacc atcggggtgc cgcagcacag cccggtcggt cctgaaatcg
     6481 accgcagcgt ggtctattcg gtagcccgga ccgaaagcgc cttcgaccag cgcgacgcgt
     6541 cgccggcaaa ggcggtcggc ctgatgcagg tcacgcccgc ggccgggcgc gataccgcca
     6601 aacggttcgg cgtcacctac gattgggaca agatggtttc cgatcccgtc tacaacacgc
     6661 agatgggcgc ggccgaactc ggcgcgctgc tacgcgaata tcgcggctgc cacatcatga
     6721 ccttcgccgg ctataatgcg ggccgcggcc gtgtccagca atgggtcaag caatacggcg
     6781 atccgcgcga tcccaacgtc gatgcggtgg attgggtgga gcgcattcca tttgccgaaa
     6841 cgcgaaacta cgtccagcgc gtgatggaga atttgcaggt ctatcgtgtt cgatttgaca
     6901 ccggcacccg ggtgatgtcg aaactcgatc agcatcgcga gacgacgcag gaagccaatt
     6961 ccgcgtcgct ttcgtcgatg cagccggcgt ttgccgagtg gtgaacccga tcacaggctt
     7021 gaccgtcggc gcatgattag cttcgaagct tccgccgcgg cgcaagacgg ccaatcgtgt
     7081 cgacaagttc gggcaggtcg gtgcgtcagg ccagggtgat atcaggccgg cgaaaccgga
     7141 gccgacaaca ccttgactcc accgaacgcg gtcacttgcc catgccggag catcacgatc
     7201 tgcgttgcga gctggcgcat ctcggcggcg tcgtggctga catagaccat cggcacattg
     7261 gcctcgtcgc gcagccgcac g
//
LOCUS       DASWGM010000761         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_924441,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000761 DASWGM010000000
VERSION     DASWGM010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924441"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VGA15_21035"
     CDS             <1..910
                     /locus_tag="VGA15_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590217.1"
                     /translation="RVAVDGKDVTGFDVRQRSVAMVYQQFINYPSMTVYDNIASPLRV
                     QGKPRAEIEKRVQEATKLLKLEPYLNRTPLQLSGGQQQRTAIARALVKGADLVLLDEP
                     LANLDYKLREELRTELPRIFEASGAIFVYATTEPSEALLLGGRTVCMWEGNILQAGET
                     SKVYRHPDTLRVAQVFSDPPLNIVGIEKRDGAVQYAGGVQAPASGLYAGLGDGAYRVG
                     FRAHQLEVANGIAGRHAFHATVTVTEITGSESFVHLNRDASNWVAVLQGVHEFPPGHV
                     LDAVLDPNNVFVFDAADRLVASPGAL"
     gene            923..2008
                     /locus_tag="VGA15_21040"
     CDS             923..2008
                     /locus_tag="VGA15_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590218.1"
                     /translation="MARIDLVDLAHSYVGNSADLESFALKPISMTWRQGGAYALLGPS
                     GCGKTTLLNLISGIVTPSRGKILFDGVDITPLSTQKRNIAQVFQFPVIYDTMSVGQNL
                     AFPLRNRGVPKGEIDARVAEIAKLLDLTPYLARKATRLTADAKQKISLGRGLVRSDVA
                     AVLFDEPLTVIDPELKWQLRSKLKALHRDLDLTMIYVTHDQTEALTFADTVVVMHEGR
                     VVQSGTPAELFDKPEHTFVGYFIGSPGMNIVPAVVRGREALIDGHTIALNRSYDALPA
                     AAKIEIGVRPEFVDVAPTAPGLLSADIERIDDLGRVRFARVRIGDAKFAARVPPGFSI
                     PGKTAGLIFDPSHVHVYADSRLVEGSA"
     gene            2008..>2187
                     /locus_tag="VGA15_21045"
     CDS             2008..>2187
                     /locus_tag="VGA15_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9590219.1"
                     /translation="MDKTINQRAWFLVLPVFLVVAFSAILPLMTVVNYSMQDTFGNNQ
                     FFWNGVGWFKELLDPS"
BASE COUNT          385 a          691 c          685 g          426 t
ORIGIN      
        1 ccgcgtcgcg gtcgacggca aggatgtcac cggcttcgac gtgcggcagc gctcggtggc
       61 gatggtctat cagcagttca tcaattatcc ctcgatgacg gtctatgaca acatcgcctc
      121 gccgctgcgg gtgcagggca aaccgcgcgc cgaaatcgag aagcgcgtcc aggaggccac
      181 caaactcctg aagctcgagc cgtatttgaa ccgtacgccg ctgcaactgt ccggcggcca
      241 gcagcagcgc accgcgattg cgcgcgcgct ggtgaagggc gccgatctgg tgctgctgga
      301 cgagccgctc gccaacctcg actacaagct gcgcgaggaa ttgcgcaccg aactgccgcg
      361 catctttgaa gcgtccggcg cgatcttcgt ctacgccacc acggagcctt ccgaggcgct
      421 gttgcttggc ggccgcaccg tctgcatgtg ggaaggcaac atcctgcagg ccggcgagac
      481 ctcgaaggtc tatcgccatc ccgacacctt gcgcgttgcc caggtatttt ccgatccgcc
      541 gctcaacatc gtcgggatcg aaaagcgcga tggcgcggtg cagtatgccg gcggcgtgca
      601 ggcgccggcg tcaggacttt acgccggcct tggcgacggc gcctaccggg tcggttttcg
      661 cgcgcatcag ctcgaggtgg ccaacggaat tgccggccgc cacgcgtttc acgccaccgt
      721 cacggtgacg gaaatcaccg gttcggaaag tttcgtgcat ctcaatcgcg atgcgtccaa
      781 ctgggtcgcc gtgctgcagg gcgtgcatga atttccaccc gggcacgtgc tggatgcggt
      841 gctcgacccg aacaatgttt tcgtgttcga cgcggccgac cgtctggtcg cttcgcccgg
      901 cgcgctgtga gggacatcca gcatggcccg cattgacctc gtcgatctcg cacactccta
      961 cgtcggcaat tcagccgatc tcgaatcgtt tgcgctcaag ccgatctcga tgacctggcg
     1021 gcagggcggg gcctatgcgc tgctcggtcc atccggttgc ggcaagacca ccttgctcaa
     1081 tttgatctcg ggtatcgtca cgccgtcgcg cggcaagatc ctgttcgacg gcgtcgacat
     1141 cacaccgctg tccacccaga agcgcaatat cgcgcaagtg ttccagtttc cagtgatcta
     1201 cgacaccatg tcggtcggcc aaaatctggc gtttccgctc aggaatcgcg gcgtgcccaa
     1261 gggcgagatc gacgcgcggg tcgcggagat tgcgaagctg ctcgatctca cgccttacct
     1321 cgcgcgcaag gcgacgcggc tgacggccga tgccaagcag aagatctcgc tcggccgcgg
     1381 actggtccgt tccgatgtcg ccgccgtgct gttcgacgag ccgctgacgg tgatcgatcc
     1441 cgagctgaaa tggcaattgc gctcgaagct gaaagcgctg catcgcgatc tcgatctgac
     1501 gatgatctat gtcacccatg accagaccga ggcgctgaca ttcgccgata ccgtggtggt
     1561 gatgcacgaa gggcgcgtgg tgcagagcgg gacgccggcg gaattgttcg acaagccgga
     1621 acacaccttt gtcggctatt tcatcggctc gcccggcatg aacatcgtgc cggccgtggt
     1681 caggggccgc gaggccctga tcgacggcca caccatcgcg ctgaaccgca gctatgacgc
     1741 cttgccggcg gctgcgaaaa tcgagattgg cgtgcgtccc gaattcgtcg acgtcgcccc
     1801 aacggcgccg gggttgctgt cggccgacat cgagcggatc gatgatctcg gccgcgtccg
     1861 tttcgcccgt gtccgaattg gcgacgcaaa attcgcagca agggtgccgc cgggcttttc
     1921 gattccgggc aagactgccg ggcttatctt cgatccgtcg catgttcacg tctatgccga
     1981 cagccgcctg gtcgagggat cagcgtaatg gacaagacca tcaaccagag ggcctggttc
     2041 ctggtgctgc cggtgttcct ggtggtggcg ttctcggcca tcctgccgtt gatgacggtg
     2101 gtgaactatt cgatgcagga tacctttgga aacaaccagt tcttctggaa cggcgtcggc
     2161 tggttcaagg aattgctcga tccgtcg
//
LOCUS       DASWGM010000762         8846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2461238,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000762 DASWGM010000000
VERSION     DASWGM010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2461238"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..284
                     /locus_tag="VGA15_21050"
     CDS             69..284
                     /locus_tag="VGA15_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590220.1"
                     /translation="MLVTLVAILCNGPLCLEKVVTNSEMSGITMMACQVNGQIGIADW
                     LAKGPYHAWTLQRYKCVAGKYIPKDEA"
     gene            complement(494..1282)
                     /locus_tag="VGA15_21055"
     CDS             complement(494..1282)
                     /locus_tag="VGA15_21055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590221.1"
                     /translation="MSLLDNPFDPAREAALVTGAGNGIGRAIAQALVGEGVRTVFADV
                     NRDTVTAAVKTSPRPELAVPFVGDLADRAARDALLADAQSALGRVTHFVHSASPARRE
                     ADHAMAVSHETWAQMHAVNLEAGFHLARELARPLIAAKEPGSFLFLTSLHSVTPRNLP
                     HYSTAKAALAMLVKELAKTFGRFGIRVNALVPGAIAAGGFVADPSLARHIPLGRLGRS
                     EDLAPMALAVLSNKVSAYVTGSAIVVDGGLSLTNWFEPPMMNGL"
     gene            complement(1384..2043)
                     /locus_tag="VGA15_21060"
     CDS             complement(1384..2043)
                     /locus_tag="VGA15_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590222.1"
                     /translation="MSRADAGSSWVNQGGKSVMAPKKAKRTTPTVCIVESLEFLQEDS
                     LKEGEIISRTLRLSEKPAHYTYLRSSDEMKAFVKEFGRSKHRYLHVSCHGNASAFFTT
                     TGRMSAADFAEMLAPHVDNRRVFLSACQAAQSEFAESLLSNSECWSVLAPVNDIYFDD
                     AAIFWSAFYHIMFKDNPDTMNRSGIERTVQKCADLINAEFRLFFREDGKVKSKTFGST
                     E"
     gene            complement(2272..2559)
                     /locus_tag="VGA15_21065"
     CDS             complement(2272..2559)
                     /locus_tag="VGA15_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590223.1"
                     /translation="MTNIVAITAANARRWQVAKIAPNRLVEVSRVAKRLTAPAAKFRY
                     QGVSQAVWGTPDRWPVVAVIHEPVGRISPTGPRKARPDDKLRRNPPPWLNP"
     gene            2861..3565
                     /locus_tag="VGA15_21070"
     CDS             2861..3565
                     /locus_tag="VGA15_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590224.1"
                     /translation="MQIVRLNNSQMDRRRFLQMMGVAAVSAGATYLALKAPRGGCYLQ
                     MGTSVTSGIAERASITPAIVGERLGMLGINAALPGACAGQNLYPHMEFRSLYCLVDAI
                     TSRDWTLQEAETEPNKAQVARLKTADFATVTYLGLEYGTNDFRYSRPIGSSGDSTRET
                     FKGGLNYSLHRLALAFPQLRLFLITPSWCTNFEDKDSDSHPNEIGAFSGSMSMRCTTS
                     APFIMSRASICGAHLA"
     gene            3593..4819
                     /locus_tag="VGA15_21075"
     CDS             3593..4819
                     /locus_tag="VGA15_21075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX9590225.1"
                     /translation="MTACTRLRKARGGAARSSRHLSRRRFDGSPTAASLARIDAHKRF
                     SMTERNNFYDCIRALAAAFVLMRHCDLLPGGSLGINMFFCLSGFLITQLLQQLPELSR
                     VNVAIFIFRRFMRVWPLMAFQVFLTLGLTVLLRSQQDVRDYIAAIPGLLTFTSSVGWV
                     NFSAAILWTLRAEFWFYVLFALAVYAAGRRAIFWIAISGIAVSWPAKYFIGHARQGKF
                     GGSFPQEFDPIFYALVYLDSLMVGALCACIVARNHPALKALYSNRVVSLWTPLALILV
                     LATLNVRAYNIAWHFKASAVAYLTGVLILHQWANPLKGDYEPLATLGRISFSIYLLHA
                     VVVDYLPLHGFPPALQFLTVGGIVVVASSLTYRWIEKPFILMSKTMAPFRPPNRILFS
                     EPADPAKTTIPFQSRP"
     gene            complement(5030..5995)
                     /locus_tag="VGA15_21080"
     CDS             complement(5030..5995)
                     /locus_tag="VGA15_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEX9590226.1"
                     /translation="MADGFWGGGAVTCAPTAPSIAIRASQRQQEVSVITRHMTKRQPR
                     LIYLGVVRAIIRISLTVALTCCSGAMATAADDLYRAQAIVTGQGEANRIIGFASCLED
                     VLIKVSGAQKLAGDRRLAAYKSNATGFVRAFSYHDQMSGTPTRDEQGTRDRPYDLIVD
                     FDEKKIDDLLKALRLKPWLSHRPIVAVFVEMQQGLKKYVVTADADQAAIQRESLLAAA
                     AKRGMTVVLPSTAALAESSINGAEFGTMTSSKLGLVAARHGGEVVLIGRLVWVDRELG
                     WATQWQMDWQSRTRRWRVRGVTFDEAFRHGIGGAAEILSNNGDPA"
     gene            complement(6434..6817)
                     /locus_tag="VGA15_21085"
     CDS             complement(6434..6817)
                     /locus_tag="VGA15_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590227.1"
                     /translation="MQKAAVVFSAAAVVAMTAVSIPTPVEARGGWGWGPGIAGGLIAG
                     ALIGGLASNAYGYGPGYGYYGPGYGYYGGYAPAYYGGYAPAYYGYGYGYGRPYYGYRR
                     AYYDYGGYRPAYYRPYYGPRFYGGW"
     gene            complement(7223..>8846)
                     /locus_tag="VGA15_21090"
     CDS             complement(7223..>8846)
                     /locus_tag="VGA15_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9590228.1"
                     /translation="LLQTLEKLATQLAGAASHADPVIDQLLSIKQLAWLVRDTGGQAA
                     LLLSNGLATGRLPPEARQNYARFIIGAEYAWNALQTMVAGTQLPPSLVEAMAATKKAY
                     FEPQYLAFSERLLNAAIAGEKLEVTSSQWRPDAVGRLGAAVTLAERALDEAKRHASAQ
                     WSSAQQSLILNMTLLAAALVLAMGSMMVVGRRVIKPLHVIRDGMLKVAAGDLSVDAGY
                     TERRDEIGSLAGALASFKQQAVEKARIEQQERERNAGAATRQQAIEGYIGEFESQVRG
                     TLTQLGDASNQMRTTSDGMSKVSTQTNASVQVAAKASGEASVNVQSVASAAEQLSASI
                     NDISRQAAHAAGIASRAVNQARSTDGTVQGLAKTANRIGEVIGLINDIASQTNLLALN
                     ATIEAARAGEAGRGFAVVASEVKSLASQTAKATDDISEQIADMQKVAGDAIEAIKGIG
                     GIIGEVNEVATAIAAAVEQQGAATQEITRSTQQAAQGTKNVSDNIVGVSAGADAAGAA
                     AQNVKTASEMLGAQTEQLRGQVDAFLGRIRAA"
BASE COUNT         1681 a         2755 c         2551 g         1859 t
ORIGIN      
        1 caccaatgct ggcatgaact ctgcatgggc tgaactgacc gggcattcct cgttaacgaa
       61 aggttaccat gcttgtcact cttgtcgcca ttctctgcaa cggcccgctt tgcctggaga
      121 aagtcgttac caacagtgag atgtccggca tcaccatgat ggcctgtcag gtaaacgggc
      181 aaatcggtat tgccgactgg ctcgccaaag gtccgtatca tgcgtggacg ttacagaggt
      241 acaaatgcgt cgcgggcaaa tacattccca aagacgaagc atgaagtgcc ccaaagacga
      301 agcatgaagt gcgcagcccc tcctatgaga ggctggtgag aggctggctt tttcgtcatg
      361 gagcgatctg atcggataag cacataaagc ggatttgcgg cagcgtcatc cgccgaaaca
      421 atcggcggcg ccatgtgctg agtttatcat cgggcgcgca ttcgcgcgac ccgttggcta
      481 ttgcgcccta ccctcaaaga ccgttcatca tcggcggctc gaaccagttc gtcagcgaca
      541 ggccaccgtc gaccacgatc gccgatcccg tgacgtaggc cgacaccttg ttcgacagca
      601 ccgccagcgc catcggggcg aggtcttcgc tgcggccgag gcggccgagc gggatgtgcc
      661 gcgccagcga aggatcggcg acgaaaccgc cggccgcgat ggcgccgggc accagcgcgt
      721 tgacccggat tccgaagcgg ccgaaggttt ttgccagttc cttcaccagc atcgcgagcg
      781 ccgccttggc cgttgaataa tgcggcaggt tgcgcggcgt gactgaatgc agcgaggtga
      841 gaaacaggaa cgagccgggc tctttggctg cgatcagcgg gcgcgccagt tcgcgtgcga
      901 ggtgaaagcc agcctccagg ttgaccgcat gcatctgcgc ccaggtctcg tggctgacgg
      961 ccattgcgtg atcggcctcg cgccgcgccg gtgacgcgct gtgaacgaaa tgcgtcaccc
     1021 gcccgagggc agactgcgca tccgccagca gcgcatcgcg agcggcacgg tcggcgaggt
     1081 cgccgacgaa aggaacggcc agttccggcc ggggcgaggt tttgacggcc gcggtgaccg
     1141 tatcccgatt gacgtcggcg aacacggttc gcacgccctc gccgaccaat gcttgcgcga
     1201 tcgcgcggcc gatgccgttt cccgccccgg tcaccagagc tgcctcgcgc gcggggtcga
     1261 atgggttgtc cagcaggctc atgctatcag gaactccctg caataagatt caacgtgggc
     1321 tgcgtgcgca gcccctcggc gacctgtcga gaatccgctc tcgcccagcg agcctcttca
     1381 gcgttactcc gtcgagccga acgtcttcga cttgaccttg ccatcttccc ggaaaaatag
     1441 cctgaattcg gcatttatca gatccgcaca tttctgcacc gtccgctcga tgccgcttct
     1501 gttcatcgta tccgggttat ccttgaacat gatatgatag aaggcagacc aaaatatcgc
     1561 ggcgtcatcg aaatatatgt cgtttacggg tgcaagtacc gaccagcact cgctgttcga
     1621 cagaagtgat tcagcgaatt cgctttgggc tgcttggcaa gccgacaaga acacccgccg
     1681 gttatcgaca tgcggcgcga gcatttcagc gaaatccgca gccgacattc ggcccgtggt
     1741 cgtgaaaaaa gcgctggcgt ttccgtggca cgacacgtga agatatcgat gtttcgaccg
     1801 cccgaactcc tttacgaaag ccttcatttc gtccgatgag cgcaaatacg tgtagtgcgc
     1861 gggtttctcc gacaggcgca gggtccgaga gatgatttcc ccctctttga gagaatcctc
     1921 ctgaaggaac tcaaggcttt cgacgatgca gaccgtcggc gtcgttcgtt ttgccttctt
     1981 gggcgccatt accgatttcc ctccttgatt tacccacgac gaaccggcgt cggcgcgcga
     2041 caccttccac tcttctacca gctcgtagca agcgtagccc ggatgaagcg gagcgaaatc
     2101 cgggacctcg ctcgccgcac accgcaatcc cggattgcgc ggagtttatc atcgggcggc
     2161 gcttcgcgcc gacccgttgg ctccatccag gctacgaaac tattctggtg agaggtaggc
     2221 ggcgttccga gacgcttctg tcgtcagccc gtagcggagc gcaatccgcc gttacggatt
     2281 cagccaaggc ggcggattac gccggagctt gtcatcgggc cgcgccttgc gcggacccgt
     2341 tgggctaatc cgccctacgg gctcatggat aacggcaact acaggccagc gatcgggcgt
     2401 cccccacacg gcttgcgaaa ccccctgata gcggaatttc gcggccggcg ccgtgagccg
     2461 cttcgcaaca cggctcactt ccacaagccg gttcggcgca atcttggcga cctgccagcg
     2521 gcgcgcattc gcggccgtaa tggcgacgat gttggtcata ggacgctcca gacatagaga
     2581 atgtcgccgg aggccctacc ggcggactgt tctaggtgag gttgtatggg atagtggtaa
     2641 ggctccgtgc gcttggtttt gcccaaccga aagaattctt ggatgcgcaa atgcatctac
     2701 gttgccgctc gagggaccgc cgccatcttg gcgctggtgt gcatcggaat atggtctgtt
     2761 gctgaccgtg gatccgacgg ggcctgttcc atctctcgct ctatcctgca ggcgtgcaca
     2821 tggcggcacg caagcgctgc cggcttgatc gaaaacctgt ttgcagatcg tgcggctaaa
     2881 taattctcag atggaccgac gacgatttct gcaaatgatg ggcgtcgctg ctgtttcggc
     2941 gggcgccacc tatttggcgc tcaaagcccc tcgcggtggc tgctatcttc agatgggaac
     3001 tagcgtcaca tctggcattg ccgagcgcgc gagcatcacg ccggccatcg tcggcgaacg
     3061 gctcggcatg ctcggcatca acgctgccct gcccggggcc tgcgccgggc aaaacttgta
     3121 tccacacatg gaattccgat cgctctattg cttggtcgac gccatcacgt cgcgcgactg
     3181 gaccttgcaa gaagccgaaa ccgagccaaa caaagcgcaa gtcgcgcgat taaagaccgc
     3241 cgattttgcc acggtgacct atctcggcct ggaatacggc actaacgatt tcaggtattc
     3301 gcgcccaatt ggatcaagcg gcgattcgac ccgcgagacg ttcaagggtg ggctgaacta
     3361 ttccctgcac aggctcgcgc tggcgttccc acagttgcga ctctttttga tcacgccttc
     3421 ctggtgcacg aattttgagg acaaggacag cgacagccac cccaatgaga ttggcgcgtt
     3481 ctcagggagt atgtcgatgc gatgcacgac atcggcgccg ttcatcatgt cccgtgcctc
     3541 gatatgtggc gcacacttgg cgtgaacgcg gacaattaca agtcccttac ctttgacggc
     3601 gtgcacccga ctccggaagg cgcgcggcgg cgcggcgagg tcatcgcgtc atttatcaag
     3661 gcgacgtttt gatgggagcc caacggccgc cagcctcgcg cgtatcgatg cgcataagag
     3721 attttcaatg acggagcgaa acaacttcta tgactgtatc cgagccctcg cggcggcctt
     3781 cgttctaatg aggcactgcg acttgctgcc gggcgggtct ctcggcataa acatgttctt
     3841 ttgtttgagc ggttttctga tcactcaact gctgcagcaa ttgcccgaat tgtcccgcgt
     3901 caacgtcgcg atattcatct ttcgtcgatt catgcgggtt tggccgttga tggccttcca
     3961 ggtttttctc actctcggct tgacggtgct ccttcggtct caacaggatg tccgagacta
     4021 cattgccgcg atccccggat tgctgacctt cacgagcagt gtcggatggg ttaacttcag
     4081 cgccgcaatt ctgtggaccc tgcgagccga attctggttc tatgtgttat tcgcgctggc
     4141 cgtttacgcg gccgggcgcc gcgcgatctt ctggatcgcg atatcaggga tagcggtcag
     4201 ctggcctgca aaatatttca ttggacacgc tcgccaaggc aagttcggcg ggagcttccc
     4261 gcaagagttc gatccaatct tctatgctct cgtctatctt gacagcctca tggtcggcgc
     4321 gctgtgcgcc tgcatcgtcg cccgcaacca tccagcgctt aaagcgctat attcaaatcg
     4381 agtcgtttca ctgtggacgc cgcttgccct gatcctggta ttggcgaccc ttaatgtgag
     4441 agcctacaat atcgcctggc attttaaggc gagcgccgtg gcctatctca cgggcgtcct
     4501 gatcctgcat caatgggcaa atccgctaaa gggtgactac gagccgctgg cgacgctcgg
     4561 ccgcatctcg ttttcaattt acctgttgca tgccgtcgtc gtggattatc tccccttgca
     4621 cggcttcccg ccggcgttgc aatttctcac ggttggcggg atcgtcgttg tcgcttcctc
     4681 gctgacttac cgctggatcg agaagccctt tatcctgatg tccaagacga tggcgccctt
     4741 tcggccgcca aacaggatct tgttttccga gcctgccgat ccagccaaaa caaccatccc
     4801 ctttcagagc cgcccgtaga tcttgatcgt gccgctgttc atgacgccgg tgctcatgaa
     4861 acacctgaaa accgtcaatc gcggaggtgt tgttccagtt cgacagggcg gatttgcggc
     4921 agcgtaatcc gccgatcagt tgtgacaggt cggtgggtca caccttcggc taagcaacct
     4981 actctacgag ctggatacga caagccggct aagtcaacct cgtgctcggc tacgccggat
     5041 cgccgttgtt tgataaaatc tcagcagcgc cgccgatgcc gtgtcggaag gcttcatcaa
     5101 aagtaactcc gcgaacccgc caccggcgcg tccggctctg ccagtccatc tgccactgcg
     5161 tggcccatcc gagttcgcga tcgacccaga ccaatcgccc gatcaacaca acttccccac
     5221 cgtgccgggc cgcgaccaaa cctaatttcg aggacgtcat cgtcccgaac tccgcgccat
     5281 tgatgctcga ttccgccaat gctgccgtgc tcggcaacac aacggtcatc ccccgcttgg
     5341 cggccgctgc gagcagtgat tcacgttgta ttgcggcctg gtctgcatca gccgtgacca
     5401 cgtacttttt caggccttgc tgcatttcga caaagactgc aacgatcgga cggtgtgaaa
     5461 gccacggctt gaggcgaagc gccttgagaa ggtcgtcgat tttcttttca tcaaagtcga
     5521 cgatcagatc gtatggccga tcgcgggtcc cctgctcgtc gcgagtcgga gtgccggaca
     5581 tttggtcgtg atagctaaat gccctgacga agcctgtcgc gttcgatttg taggctgcca
     5641 atcggcggtc gcctgcaagc ttctgcgcgc cggatacttt aatgagaacg tcctccaaac
     5701 aggaggcgaa gccgatgatg cggtttgcct cgccctggcc cgtgacgatt gcctgcgctc
     5761 gatataggtc atccgccgcg gtcgccatcg cgccgctgca acacgtcaac gcaactgtca
     5821 gcgatatcct gatgatggcg cgaaccacac caagatatat caagcgcggc tggcgctttg
     5881 tcatatgccg agtaatcacg ctcacctcct gctgtcgctg cgacgcccga atagcaattg
     5941 aaggagccgt tggtgcacag gttactgcgc cccctcccca aaaaccatcc gccatctttt
     6001 tctcgtatcc atatactccc tcacccgatt gatgcggtcg cctgcgacga caaagacaag
     6061 gcagcccggg cggatcagca agcgtagccc ggataagcga agcgacatcc ggggggttat
     6121 tctgttgatc cccgcatatc gctgcgctca tgcgggctac ttgctcttcc accagctcgt
     6181 aaggcggatt agcgaagcgc aatccgccag taacgcgagc ccagtgcaat atgctcgcta
     6241 ttgcgcccta cggtgccgag aggatcaagg ctggtttcgg ataggggaaa aaatacacgg
     6301 atcgaaagcc gcgaaggtcg aacgctgccg gcttcttgag tgtcaacttt ccgctgttgt
     6361 gaatgcggtt ctgcaacgag cagcagccgg atgtcggaac cggcggcagc aacgaggctt
     6421 gggcagttgg ttcctaccag ccgccgtaga aacgcggacc gtagtacgga cgatagtagg
     6481 ccgggcgata tccaccataa tcataataag cccgccgata tccgtagtac ggtcggccat
     6541 atccatatcc atatccataa tatgcggggg cgtagccgcc gtagtaggcg ggcgcatagc
     6601 caccgtagta gccatagccg ggtccgtaat acccatatcc cgggccgtat ccgtatgcgt
     6661 tagaagccag accgccgata agggcaccgg caattaatcc gcctgcgata cccggacccc
     6721 agccccaacc tccacgcgct tcaaccggtg tcggtatgga gactgcagtc atggccacta
     6781 cggctgcggc ggagaaaaca actgctgcct tctgcatggg ctttcctttc accaaaatgt
     6841 tgtcgattaa caaaagtcga attcgatcgt tgcgtccgca agggccaccg gaacgttctc
     6901 attgaattta atgttgatga atgtccgctg gtttgcgagg gatgtcgaaa ttttcaaata
     6961 ccttcgccta gcttagttga tagccacgaa ccaaacggac agaaaaccgc tccccgcttt
     7021 ttgccgggat gaaccctacg ccagccgcct tcaaatacgc cgctggtcta tacgctaagc
     7081 tcgtagggcg gattagcgca gcgtaatccg ccgttacgga tgcagccaag gcggcgcaat
     7141 acgcggagcc cgtcatcggg ccgcgccttg cgcggacccg ttgggctaat ccgcccgggc
     7201 tacaagactc cactgcgccc gatcaggccg cgcgaatcct gccgagaaaa gcatcgacct
     7261 gaccgcgcag ttgctcggtc tgggcgccca gcatctccga ggcggttttt acattctgcg
     7321 ccgccgcgcc ggctgcatcc gcgcccgcgc tgacgccgac gatgttgtcg gacacgttct
     7381 tggtaccctg cgccgcctgc tgggtgctgc gggtgatttc ctgggtcgca gcgccctgct
     7441 gttcgactgc cgcggcgatc gcggtggcga cctcgttgac ttcgccgatg atgccgccga
     7501 tccccttgat cgcctcaatc gcgtcgccgg ctaccttctg catatcggcg atctgctccg
     7561 aaatgtcgtc ggtcgccttt gcggtctggc tcgccaggga tttgacctcg gacgccacca
     7621 ccgcaaaacc ccgcccggcc tcgccggcgc gggcggcctc aatggtggcg ttcagcgcca
     7681 gcaggttggt ctgcgaggcg atgtcgttga tcaggccgat cacttcgccg atccggttgg
     7741 cggttttggc caagccctgg acggtgccat cggtcgagcg cgcctgattg acggcgcggc
     7801 tggcgatccc ggccgcatgc gcggcctgcc tcgaaatatc gttgatcgat gcgctcaact
     7861 gctccgcggc ggacgcgacg ctttgcacat ttaccgacgc ttcgcccgag gccttggccg
     7921 cgacctgaac gcttgcattg gtctgcgtgg aaactttcga catcccgtcc gaggttgttc
     7981 gcatctgatt ggaggcatcg ccgagttggg tgagcgtgcc gcgcacctgg ctctcgaact
     8041 cgccgatata gccctcgatg gcctgctgtc gcgtcgcggc gccggcattg cgctcgcgct
     8101 cctgctgctc gatccgcgcc ttctcgactg cctgctgctt gaagcttgcc agcgctccgg
     8161 caagcgatcc gatctcgtcg cgacgctcgg tgtagccggc atctaccgac aaatcgcccg
     8221 cggccacctt gagcatgccg tcacgaatga cgtgcagcgg cttgatcacc cggcggccga
     8281 ccaccatcat gcttcccatc gccagcacga gggctgccgc gagcaacgtc atattgagga
     8341 tcagcgattg ctgcgccgaa gaccactgcg ccgatgcgtg gcgcttcgcc tcgtccagcg
     8401 cgcgctcggc aagcgtgacc gcggcgccga ggcgcccgac cgcatcgggc ctccattggc
     8461 ttgacgtcac ttccagtttc tcgccggcga tcgccgcatt cagcaatcgc tcagagaacg
     8521 ccagatattg gggctcgaaa taggccttct tggttgcagc catcgcttcg accaggctgg
     8581 gcggcaattg cgttcccgcc accatggtct gcagcgcatt ccaggcgtac tccgcaccga
     8641 tgatgaatct tgcgtagttc tgacgcgcct cgggcggcag gcggccggtg gcaagtccgt
     8701 tcgagagcaa caaggcggct tgaccgccgg tatcgcggac cagccaggcc agctgcttga
     8761 tcgagagcaa ttgatcgatc acggggtcgg cgtggcttgc ggcaccggcg agctgcgtgg
     8821 ccagcttttc cagcgtctgc agcaac
//
LOCUS       DASWGM010000763         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1884067,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000763 DASWGM010000000
VERSION     DASWGM010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884067"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1726
                     /locus_tag="VGA15_21095"
     CDS             227..1726
                     /locus_tag="VGA15_21095"
                     /inference="COORDINATES: protein motif:HMM:NF009120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9590229.1"
                     /translation="MNPKLKPTGLRPALLAVAGLAALIAAADPVGATDRRSERVVESI
                     EQRAAGEPVMAIVSLHSQRITVYDANGWILRAPVSSGQKGRETPAGIFSVIQKEAEHY
                     SNLYDDAFMPHMQRITWSGIALHGGALPGYPASHGCVRMPFDFAARLFDATRPGLRVI
                     VAPTDVAPIEIAHPALFQSKPGAGALAAARTAEADEAARKADQARLAAGTAFREAAQA
                     MAPVRVAENLKTRAEAQLAAAETALGSAISAEAKQQAERDKTQAAARIAALQLQLASA
                     KAELQPKQDAVTRTREAVAIAETARAAAAEAARQVARQFEPVSVLISRKTQRLYVRQA
                     FEPILESPVTILDADSPIGTHVFTAMERTNSDTNMRWSVVSLDGGRSQSGVVEPQGRA
                     RGGHGRDLEPMPTDPGSAKTALDRIVIPQDALDRIAGMAPRSSLIITDEALSPETGKG
                     TDFVVLLSGEPQGGIKMRRRGPEAEFPYARARDRMPFWRSPFGGPFFNW"
     gene            complement(2578..>3111)
                     /locus_tag="VGA15_21100"
     CDS             complement(2578..>3111)
                     /locus_tag="VGA15_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9590230.1"
                     /translation="GLGLSMVYGFVKQSNGHIRIYSEEGHGTTVKLYLPQAAGVADAL
                     PAEAGISEFVRGDESILIVEDDALVREYVVTQISRLGYDTLAANNAAEALAIINGPER
                     IDLLFTDVIMPGGMNGRQLAIESQTRRPGLKILYTSGYTENAIVHHGRLDAGALLLPK
                     PYLSSDLALMIRTALAS"
BASE COUNT          587 a          956 c          996 g          572 t
ORIGIN      
        1 cgacgagatc acggtcgatg gcggctacgt caatatgctg atgaacctgg tgccgcggcc
       61 gggctttgag tgaaggggcc gtgcgctaaa tctgcactag ccgagcgctc gcagtaagca
      121 ttcgggaagt tccgggttgg caggttcgac ctgatggcgt tatgaatctc ctgagtgcaa
      181 gcaggcttag ccccgatcaa atgagacgcg catgaggtct cgttgtgtga acccgaagct
      241 taagccgaca gggctgcggc ccgcgcttct tgccgttgcg ggtctggccg cgctgatcgc
      301 ggccgcagat cctgtcggcg ccaccgaccg ccggagcgaa cgcgtcgtcg agtccatcga
      361 gcagcgcgca gccggcgagc cggtcatggc gatcgtttcg ctacatagcc agcgcatcac
      421 cgtctatgac gccaatggct ggatcctgcg ggcgccggtg tcgagcggcc agaagggacg
      481 cgagacgccc gccgggatct tcagcgtgat tcagaaagaa gcggagcact actcgaacct
      541 gtatgacgat gccttcatgc cccacatgca acgcatcacc tggtccggca tcgcgctcca
      601 cggcggcgcc ctgcccggat atccggcgtc ccacggctgc gttcggatgc ccttcgactt
      661 cgcagcccgg ctgttcgacg ccacccggcc gggcttgcgg gtgatcgtgg caccaaccga
      721 tgtggcgccc attgagatcg cccatccggc tttgtttcag tcgaagccgg gtgccggtgc
      781 cttggccgcc gcccgcaccg cagaggcaga tgaggcggcc aggaaggcgg accaggcgag
      841 actcgccgcc gggacggcct tcagggaggc cgcgcaggcg atggcgccgg tgcgcgtggc
      901 ggaaaatctg aagaccagag ccgaggcgca attggcggcc gccgagaccg cgcttggctc
      961 cgccatctcg gccgaggcca agcagcaggc cgagcgcgac aaaacgcagg ccgccgccag
     1021 gatcgcggcg ttgcagctgc aattggccag cgcaaaggcg gagctgcaac cgaagcagga
     1081 tgccgtcacg cgtacgcgtg aagccgtcgc catcgcagag acggcgcggg ccgcggcagc
     1141 cgaggcggcg cgccaagtgg cgcgacagtt cgagccggta tcggtgttga tcagccgcaa
     1201 gacccagcgg ctttatgtcc ggcaagcctt tgagcccatc ctggagagtc cggtcacgat
     1261 cctggatgcc gacagtccga tcggcaccca cgtcttcacc gccatggagc ggaccaacag
     1321 cgacaccaac atgcgatgga gcgtcgtctc actggacggc ggacgttcgc aaagcggcgt
     1381 ggtcgagcca caaggccggg cgcgcggggg ccatggtcgc gacctcgagc cgatgccgac
     1441 ggacccgggc agtgcaaaga ccgcgctcga ccggattgtc attccgcagg acgcattgga
     1501 tcgtatcgcc gggatggcgc cgcgatcctc ccttatcatc accgacgagg cgctgagccc
     1561 cgagaccggc aaaggcacgg atttcgtggt gctcttgagc ggcgagccgc aaggcggcat
     1621 caagatgcga cgacgcggtc cggaggccga attcccttac gcacgcgcac gcgatcgcat
     1681 gcctttttgg cgctcgccat ttgggggtcc gttttttaac tggtgagcct gttcttttca
     1741 cttcgggtaa cggcacaaag ctggcgtgcg cgtcccctcg atgaatgtcc gctgtcgagc
     1801 gaagagcgga catcgatcag tcgatgctcg aagtcggttt ttgtgaaggt ttgcggatgc
     1861 cggccccatg aaggtggagc ggcgcgcacc ggggccggcg tacgcaaacc tccaatggag
     1921 gaaaccgcgg gaggtgtggc gaggtggcgc gtcagacgct atggcgattt gacggctgtc
     1981 gtggctgaat gcgagtacaa cttttgcgat atggcacgaa ctttgcgaat gggtgaaaac
     2041 acctttcgtc gtccctgtaa tttcaaagag ttacgcgttg taaaacgggc acatattgca
     2101 acttttgcgt gatgtgcctg atggcagcct ttgtaccgca tcggccgaac aaatatcggc
     2161 cgaacaaata ttgccacgtg acagcgtcca acggaattat tttaagctcg ccttatacac
     2221 cactcgcgat catgactgca tctccaatcg catcggttcg ggtattcgaa cctgtccgcg
     2281 atgcggatgt cggctcgcta cgttcggtgc gctcccaacg ccgtcgacga tatatcgtga
     2341 gatcatcatg ctgaacaatc atcggttctg gggacatgct tctggcccct catcatcatc
     2401 aggtaagacg ctctcatcgt tccatccatt gctggcacgg ccatgccgca gcgccctatc
     2461 gcgcgagcgg tttagtcctt tgacccaaag ccgaccaacc ccatcggctg atgccggtcc
     2521 ggtgttggac gcgccctacg ccactcctta gatcaaggag cgcagcgtcc ggaataatca
     2581 cgacgccagc gcggtccgaa tcatcagcgc cagatcggag ctgaggtagg gcttcggcag
     2641 cagcagcgcg ccggcgtcga gccggccatg atgcacaatg gcgttttcgg tataacccga
     2701 cgtgtaaagg atcttgagcc ccgggcgtcg cgtctgcgac tcgatcgcga gctggcggcc
     2761 gttcatgccc ccgggcatga tcacgtcggt gaacagcaga tcgatgcgtt caggaccatt
     2821 gataatcgcc agcgcttcgg cggcattgtt ggcggccagc gtgtcgtaac cgagacggct
     2881 gatctgcgtc acgacgtatt cgcggaccag cgcgtcgtcc tcgacaatca ggatggattc
     2941 gtcgccgcgt acgaattcgg agatgccagc ctctgcgggc agagcgtccg cgacgcccgc
     3001 ggcctgcggc aggtaaagct tcacggtggt gccgtggccc tcttcgctgt agatcctgat
     3061 atgtccgttc gattgtttga cgaagccgta gaccatgctg agaccgagcc c
//
LOCUS       DASWGM010000764         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1884087,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000764 DASWGM010000000
VERSION     DASWGM010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884087"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VGA15_21105"
     CDS             <1..937
                     /locus_tag="VGA15_21105"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX9590231.1"
                     /translation="TPDRAGRVADIVLGRDDLEGYIALRRFLGATIGRYANRVANGAF
                     ELDGHRFQLPTNDGANALHGGLEGFDRKSWAITATGEKPVPFVTLSYVSPDGEEGYPG
                     RLQANITYSISGSMELSAAFSAETDKPTIVNLTNHSFFNLAGVETGGGILDHHLTIAA
                     DTYLPVSRAGIPLGAPGRVDGTPFDFRIRHRVGVRLHDVNEQLQIRQGYDHNFCLRGG
                     VTSVPRLAARLEDPRSGRVLELLTNQPGVQFYSGNFLDGTVTGKYGRVHRQYDALCLE
                     PQAYPDTPNRPDYPSARLDPGQTYRHSSLYRFSAS"
     gene            965..1846
                     /locus_tag="VGA15_21110"
     CDS             965..1846
                     /locus_tag="VGA15_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9590232.1"
                     /translation="MEEVATTILSRHHCLLGEGCTYDPATDTAWWFDILERTLFRADL
                     ASGVVTSHALPVMASALAFIDDQRQLLATENGLYVRDVTDGRLTLHTPIEADNVATRS
                     NDGRVHCCGALWIGTMGRKAEKGAGAIYRFHGGELVRLYAGVTIPNAICFSPDGATAY
                     FTDSSEGILHRVAIDPANAMPIGNPAILYDHRGGVGGLDGAVVDAEGLIWNARWGGSC
                     IDVYTPEGNRARTIRVPATQPSCPVFVGRNFDRLLVTTASEGMDELAKAADPQHGMTF
                     SLDVGATGRPEPRVRLT"
     gene            2036..2278
                     /locus_tag="VGA15_21115"
     CDS             2036..2278
                     /locus_tag="VGA15_21115"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX9590233.1"
                     /translation="MQRIKHPPMRNKISLADPTQVRAWTRRLAISADSLKAVIDKVGN
                     SVAAVTKEVELQRAAHSTSPAPPIERPPTAGEPAPA"
     gene            complement(2377..>2641)
                     /locus_tag="VGA15_21120"
     CDS             complement(2377..>2641)
                     /locus_tag="VGA15_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590234.1"
                     /translation="LMPGLRSERARDHARDLLAKVYPIPEDAEVRRLRQLAIDYIQEA
                     ERLEAENSGRAAESEELISAALDAVARDYFKRALLRAVSRRIR"
BASE COUNT          488 a          831 c          805 g          517 t
ORIGIN      
        1 cacgccggat cgcgccggcc gcgttgccga catcgtcctt ggccgcgatg accttgaagg
       61 ttatatcgcg cttcggcgct tcctcggcgc gacgatcggg cgttatgcca accgtgtcgc
      121 gaacggtgcg ttcgaactgg atggccatcg ctttcaattg ccgaccaatg acggcgccaa
      181 cgcgcttcac ggcggtctgg agggtttcga tcgcaaaagc tgggcgatca cggccacggg
      241 cgagaagccc gtgcctttcg tcacgctgtc ctatgtcagt ccggacggcg aggaaggcta
      301 tcccggcagg ctgcaagcca acatcaccta cagcatttcc ggtagtatgg aactttcggc
      361 cgcgttctcg gcggagaccg acaagccgac tatcgtcaat ctgaccaatc acagcttctt
      421 caatcttgcg ggcgtcgaga ccggcggcgg cattctggat catcacctca ccatcgcggc
      481 ggatacttac ttgccggtga gcagggcggg catcccgttg ggcgcgccgg gccgggtcga
      541 tgggacgccg ttcgatttcc ggatacggca tcgcgtcggc gtgcgcctgc acgatgtgaa
      601 cgagcagctt caaatccgcc agggctacga tcacaatttc tgtctgcgcg gcggcgtcac
      661 ctccgtaccg cgactggctg cccgtctcga agacccgcgc tccggccgtg tcctcgaact
      721 cctgacaaat cagccgggtg ttcagtttta ttcaggcaat ttcctcgatg ggaccgtgac
      781 cggaaaatat ggccgcgttc atcgccagta tgacgcgctg tgcctggagc cgcaggccta
      841 tcccgacacg ccgaatcggc cggactatcc ctcggcgcgg ctcgatcccg gacagaccta
      901 tcgtcacagc tcgctgtatc gattttccgc gagttgacgg cgcggaggga cgacggaaaa
      961 tcgcatggag gaagtcgcta ccacgatcct ttcccggcac cattgcctcc tgggcgaggg
     1021 ctgcacctat gatccggcga cggataccgc ctggtggttc gatatcctcg agcgaacctt
     1081 gttccgggcc gatcttgcat cgggcgtggt tacctcgcat gcgcttcccg tcatggccag
     1141 cgcgctcgct ttcattgacg accaacgcca gttgctcgcg accgaaaacg gcctctatgt
     1201 ccgcgacgtc acggatggcc gccttacgct gcatacgccg atcgaggccg acaatgtggc
     1261 gacgcgctcg aacgatggac gagtccattg ctgcggcgcg ctctggatcg ggaccatggg
     1321 ccgcaaggcc gagaagggcg cgggcgcgat ctatcgcttt catggtggcg aactggttcg
     1381 gctttatgcc ggcgtgacca taccgaacgc catctgcttt tcgccggatg gcgcaacggc
     1441 ctatttcaca gatagcagcg agggcatcct tcatcgcgtc gccatcgatc ctgcgaatgc
     1501 catgccgatc ggaaatcccg cgatacttta tgaccatcgc ggcggggtgg gcggtcttga
     1561 cggcgccgtg gtcgatgccg agggactgat ctggaatgcc cgctggggtg gatcatgtat
     1621 cgatgtctac acgccggaag gcaaccgcgc ccgcaccatt cgggtgccgg caacgcagcc
     1681 gagctgcccg gtcttcgtcg gcagaaattt cgatcggttg ctggtcacga ctgccagtga
     1741 gggaatggac gagctcgcca aggcggccga tccgcagcat ggaatgactt tcagtctgga
     1801 tgtcggtgcc accggtcgtc ctgaaccccg cgtcaggctg acttgatgaa aaaagcgccc
     1861 gtctcttgcc cgtcgattgc cgcgtgagag aaccggcctg ccgcgttttt gagagcgcgc
     1921 aggttggatt agacaacggc gttcgggcgg ttcctttcgg ctagggagtc gggcgcatgc
     1981 gaaaatggaa cgaaggcctt tgcatgccgt tgatccgcag ccagggaatg ccgccatgca
     2041 acgcatcaaa catccgccca tgcgcaataa gatcagcctg gcagatccaa cccaggttcg
     2101 tgcctggacc cggcggctcg caatttcggc cgactcgctg aaagccgtca tcgacaaggt
     2161 cggtaactct gtcgcggccg tgaccaagga agttgaattg cagcgggcgg ctcactcgac
     2221 ttcgccggcg cctccgatcg agcgcccgcc taccgcaggt gagccggctc cggcttgacg
     2281 gacaagcggg catccaagct tcatgagtgc atctgcctta aagcctgaag cgtttttacc
     2341 aggacaacgg aaccgatcga ttccggtaag cttgcctcaa cgaatccggc gcgacacggc
     2401 ccggagcagg gcacgtttga agtaatccct tgcgaccgca tccagggcgg ccgagatcaa
     2461 ttcctcggat tccgccgcac gaccgctgtt ctcagcctca agccgctcgg cttcctgaat
     2521 atagtcgatc gcgagctggc gcagccggcg gacctcggca tcttcgggaa ttggataaac
     2581 cttcgccagc aaatccctgg cgtgatcacg ggcgcgttca cttctcaggc cgggcatcag
     2641 c
//
LOCUS       DASWGM010000765         7056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1041434,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000765 DASWGM010000000
VERSION     DASWGM010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041434"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VGA15_21125"
     CDS             <1..508
                     /locus_tag="VGA15_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9590235.1"
                     /translation="PTGGGTGLIGMWKAFAELEAIGFIGSRRPRMVAVQAAGCAPMVR
                     AFEDGTEHAARWEDAHTIASGIRVPQAIGDFLILRAVRESKGFAIAVTDEEISAALNE
                     VAREEGLLLCPEGAATYAAYKQSLADGRVTKNDRVMLFNCATGLKYPLPPITRTLDRH
                     QPIDYARL"
     gene            594..1565
                     /locus_tag="VGA15_21130"
     CDS             594..1565
                     /locus_tag="VGA15_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HEX9590236.1"
                     /translation="MRRTVWAALIALLISGGSALAENFPSHPITIVVPFSAGGPSDAM
                     ARILAERMKVTLGEALLVENVTGAGGSIGVARAVRSPPDGYTISFGHLGTHVANGAIY
                     KLGYDLVSDLEPVVLLPSNPMIVVSKNAVPAKSLKELMAWLKARPEPATAGTAGAGSG
                     SHIAGLYFENVTGIKLQYVPYRGTAPAMNDLIAGQIDLIVDQTSNSIGQVRAGNIRAY
                     AITADKRLESAPDVPTTDEAGLPGFHMTLWSGLWVPKDTPKDIVAKLNAAAVDALNDP
                     AVRRQLENLGLQMPPADQLSPEALGSWQKAEIAKWWPMLKAANVKVD"
     gene            1823..3046
                     /locus_tag="VGA15_21135"
     CDS             1823..3046
                     /locus_tag="VGA15_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX9590237.1"
                     /translation="MSKYRIGIAVVVLTTLLSSAALARVRLGIGPLGVARFAVARVLS
                     LGGLHHARAFARHGRIRTASLRSQDVRNATDSGLGNPAVRRQIVAAAALAGWHGGHIT
                     NGWWRHGDGGYGWVGPLFWPFAIYDIYDYAIWGDGIGFWDYGYPDIYAAIFAPYGHDD
                     LAAYAGPSPFGRRYRRVPPLQQLCGDDSREIAGLPIDQIQQAIQPNEAQRATLDDLAN
                     ALISAAQMIRASCPTQTAFTAPDRLAVMQQRIEAMIKAELAVEQPLGKFYDLLDDEQE
                     ARLNASAEDRRKMSATNGATEAPAQGCGAAQPAALQWPADEIEARLHPNDTQRAALKV
                     LQDANARAVDILNAGCQPKDATTPPARLDAVEGRLEAMQRAVYLVSAALEGFYATLSD
                     EQKAQFEAIGQKRTA"
     gene            complement(3356..4183)
                     /locus_tag="VGA15_21140"
     CDS             complement(3356..4183)
                     /locus_tag="VGA15_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9590238.1"
                     /translation="MMKIRYAVVLVAAMIVAEPCPAVENSIVLASTTSVEASGLLANI
                     LPKFMAKTGIRVDVVAQGTGKALDTARRGDADVLLVHDPEAEEQFMSEGHGSTRRQIA
                     WNDFIIVGPTADPAHVRGGSDSVAALKSIASAQASFVSRGDKSGTNAAELRLWKIAGQ
                     TPDVLGKEKWYRDVGSGMGQALNVASAMDAYTLSDRGTWLSFNNKGSLIIVVEGDPRL
                     VNRYDVIELNGQKHASAKLDAAKVFADWLVSSEGQQAIGAYQVSGQKLFNPSANSPK"
     gene            complement(4180..4839)
                     /locus_tag="VGA15_21145"
     CDS             complement(4180..4839)
                     /locus_tag="VGA15_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590239.1"
                     /translation="MQDFTQIIGLSVGVSLAATTLAAAIGLPIGTALAIYPFCGRRLI
                     VVLMNAFFGLPPVVVGLVLYLALSRSGPLGSLALLFTPTAMIIAQATLAIPIITALVH
                     RASERAWARYGDELIADGATRLQAAPQILMIVRADLVTAILAGFGRTVSEVGAVILVG
                     GNIRGLTRTMTTTIALQTSQGDLSLALALGAVLVGISISISATVFALAGRARSAGNDE
                     V"
     gene            5286..5924
                     /locus_tag="VGA15_21150"
     CDS             5286..5924
                     /locus_tag="VGA15_21150"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX9590240.1"
                     /translation="MTDLGEIKRDRPCNRRWLLRHGALALPFILVGIPARAVERVGSV
                     EDVKGEAFAELEAVRRTLDRAAPVFLGDEVVTGVASRLGMRLGRDTTIRLGEQARLKI
                     DRFLVDAGGEMTLRSGPLLFDRPPRNARAGVQIRSPFALIAVRGTQFFAGPSNERFGV
                     FVARGSVAVTSVGQQVILREGEGTDFVSPGTPPTPVKRWAPQRIRAALASVS"
     gene            6037..>7056
                     /locus_tag="VGA15_21155"
     CDS             6037..>7056
                     /locus_tag="VGA15_21155"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX9590241.1"
                     /translation="MGRMRRALQQRLPVAAGIATLTVFLAAGILLPKTTSEVLRDSAF
                     DIELAGDQWLRRPAPSDLKVIVVDIDRASIDALGTWPWPRETMARIVEAVATGRPAAI
                     AIDVLFAEPDDRSPAALARRLGSVTGRAEISTLGESLPDGDKRLARAIGSVPVALGFV
                     LDPDRHGTLPGAAIASRGSLPFDDLWQAAGAIGPTLPLAAAASGLGALSLPGSADGAI
                     RLVPVFVAAGRVLMPGLAAEALRLASGASSYLIKGEEPPTLVIGSRRVALSRDGLLRL
                     VPVAAGRRVARTLSAVDVLEGRADGGRLAGALVLLGGSAPELGGLRKTPADPLTPSVQ
                     IQADAV"
BASE COUNT         1346 a         2202 c         2213 g         1295 t
ORIGIN      
        1 tccgaccggc ggcggcaccg gcctgatcgg catgtggaag gcgttcgccg agctcgaagc
       61 catcgggttc atcggctcca ggcggccgcg gatggtggcg gtgcaggcgg cgggctgtgc
      121 gccgatggtg cgcgcctttg aggacggcac cgagcacgca gcccgctggg aggatgccca
      181 caccatcgcg tccggcatcc gcgttcccca ggcgatcgga gattttctga tcctgcgcgc
      241 ggtgcgcgag agcaaaggct ttgcgattgc ggtgacggat gaagagattt ctgctgcgct
      301 gaacgaagtc gcgcgtgagg aaggcttgct gctgtgtccg gaaggtgccg cgacctacgc
      361 cgcctacaag cagagtctcg ccgacggccg ggtgacgaaa aatgatcgcg tgatgctgtt
      421 caattgcgcg acaggcctta aatacccgtt gccgccgatc acgcgcacgc tcgaccgcca
      481 ccagccgatc gactacgcca ggctgtagct atcgccaagg cgaagctgta gctatcgcag
      541 cgcgaagctg tagctatcgc agcttgaatg gtgcggcagt gttggagggt gtgatgcgaa
      601 gaacagtctg ggccgcgttg attgcgctct tgatatcggg cggcagcgcg ctcgcggaaa
      661 actttccatc gcatcccatc accatcgtgg tgccgttttc cgccggcggc ccctccgacg
      721 ccatggcgcg gattttggcc gagcgcatga aggttacgct cggcgaagcc ctgctcgttg
      781 agaatgtgac gggtgccggc ggctcgatcg gcgtcgcgcg cgcggtgcgt tcgccccctg
      841 acggctacac catcagcttt ggccatctcg gcacccacgt cgccaacggc gcgatctaca
      901 agctcggcta tgacctcgtc agcgatctcg agccggtggt gctgctgccg agcaacccga
      961 tgatcgtcgt cagcaagaac gcggttccgg ccaaatcgtt gaaggagctg atggcgtggc
     1021 tcaaggcgcg ccccgagccg gcgacggccg gcaccgccgg cgccggctcg ggaagtcaca
     1081 tcgccgggct ctattttgag aacgtcaccg gcatcaagct gcaatatgtg ccgtatcgcg
     1141 gcacggcgcc ggcgatgaat gatctcatcg ccggacagat cgatctcatc gtcgatcaga
     1201 cctccaattc catcggccag gtgcgcgccg gcaacatccg cgcctatgcc atcaccgccg
     1261 ataagcgcct cgaatcggcg ccagacgttc caaccaccga cgaagcgggt ctgcccgggt
     1321 ttcacatgac tttgtggtcc ggcctctggg tgccgaaaga cacgccaaaa gacatcgtcg
     1381 caaagctcaa cgctgcggcc gtggatgcgt tgaacgaccc ggcggtgcgc aggcaactcg
     1441 aaaacctggg gctgcagatg ccaccggccg atcagctctc gccggaagcg ctcggcagct
     1501 ggcaaaaggc cgagatcgcg aaatggtggc cgatgctcaa ggccgccaac gtcaaggtgg
     1561 actagaccag ggcatggact agatgcatga tccggaaaag tggttaccgg ttttccgaaa
     1621 agatcatgct caaacaaaaa gatagagcgg gatgacgatt cgaagaatag tcatcgcgct
     1681 ctagtaagca atgcggattt ctacctctac cggaagtggc tgacccatgg tcagatggtc
     1741 tgcatagcaa acgcagggaa ctttcgcttc ctctcggcgt catctaaaag tgacacgatg
     1801 aggcctggcg caggagggtg ccatgtcgaa gtaccgcatt ggaattgccg ttgtcgttct
     1861 gacaaccctg ctgtcgagtg cggccctggc tagagtgcgt cttggcatcg gtccccttgg
     1921 ggtcgcgaga ttcgcggtag cccgcgtatt gtcgctgggg ggattacatc acgcacgtgc
     1981 ctttgcccgt cacggccgca tccgaacggc ttccctcaga tcgcaagacg tccgcaacgc
     2041 tacggattcc gggcttggca acccggcggt gcgcaggcaa atcgtcgcgg ctgcagcgct
     2101 ggcgggctgg cacggcggcc acatcacgaa cggatggtgg cgacacggcg acggcggata
     2161 tggatgggtc gggccgctgt tctggccatt cgccatttac gatatctacg actacgccat
     2221 ctggggtgac ggcatcggct tctgggacta cggttatcct gacatctatg ccgcgatctt
     2281 cgcgccttac ggccatgacg atctcgccgc ctatgcggga ccgagcccgt tcggccgaag
     2341 atatcgcaga gtccctccgc tacagcagct ctgcggcgat gacagccgcg aaattgccgg
     2401 cctccccatc gatcagattc agcaggcgat acagccgaat gaggcgcagc gcgccacctt
     2461 ggacgatctt gccaacgcat tgatctcggc cgcccagatg attcgggcgt cgtgtccgac
     2521 gcagacggcg ttcacagcgc cggaccgatt ggccgtcatg cagcagcgta tcgaggcgat
     2581 gatcaaggca gagttagccg tggagcagcc gctggggaaa ttctacgacc tgctggatga
     2641 cgagcaggaa gcacggctca atgcgtcagc cgaagaccgg cgcaagatgt ccgccacaaa
     2701 cggggccacg gaagcgcctg cccaaggttg cggcgcggcg caaccagccg cgttgcaatg
     2761 gccggcggac gagatcgagg ccaggctgca cccgaacgac actcaacgcg ccgctcttaa
     2821 agtgctgcaa gacgccaatg ccagggctgt cgacatcctg aatgctggat gccagccgaa
     2881 agacgcgacc acaccacccg ctcgccttga cgcagtggaa gggcggctcg aagccatgca
     2941 gcgagcggtc tatctggtga gcgccgcgct cgaaggcttc tatgctacac tgagcgacga
     3001 gcagaaggcg cagttcgagg cgatcggaca aaaacgaacg gcgtaatttc ccatccattg
     3061 ccatcctgag aggcagcccg attcaccacg gtcacgccag caaccgacgc gatcgacccc
     3121 ggaacagacg tcggtggggc gacaacggac cagcgtctac ggcaacaccg tattcgctta
     3181 ggtcaggacg atttcgctac gacatggcct tgctctaaca ccaagaccat caccggaaag
     3241 cggctcgacg cgagccgtaa gtcacgcgcc gagcctagcc gaacgaggtc agggctactg
     3301 aacagtcggt tcggctgtcg gtagaataat tggtcggcct gcaattgaaa gtagattact
     3361 tgggagaatt cgcagacggg ttgaacaatt tttgaccgct cacctgatac gcgccgatgg
     3421 cctgctgccc ctctgatgaa accagccaat ccgcgaagac cttcgcggcg tcgagcttgg
     3481 cactcgcgtg tttctgcccg ttgagttcga tcacgtcata tcgattgacc aaacgagggt
     3541 cgccttcgac aacgatgatc aacgatccct tattgttgaa gctgagccat gttccccggt
     3601 cggacaacgt gtatgcatcc atcgcgctcg cgacattaag ggcttggccc atcccgctgc
     3661 caacgtcgcg ataccatttt tccttgccga gcacgtccgg cgtttgaccg gctattttcc
     3721 aaagccgtaa ctccgccgcg ttcgtgccgc tcttgtcgcc tcgcgaaacg aacgatgctt
     3781 gcgccgacgc tatcgatttc aacgccgcga cactgtcact gccaccgcga acgtgagccg
     3841 gatcggcagt cggaccgact atgataaagt cattccatgc gatttgccgc cgcgttgagc
     3901 cgtggccctc gctcatgaac tgttcttcgg cttctggatc gtgaacgagt aacacgtccg
     3961 catcgcctcg gcgcgctgtg tccaacgcct tgccagtgcc ctgcgcgacc acatcgaccc
     4021 tgatgccggt ctttgccatg aacttcggca ggatgttcgc caacagtccc gacgcctcga
     4081 ccgaagttgt cgaggcgagg acgatggagt tctcaacggc tgggcagggc tcggctacga
     4141 tcatcgcagc cacaagcacc actgcgtatc ggatcttcat catacttcgt cgttccccgc
     4201 tgatcgcgcc cgtccagcca atgcgaagac cgtggcgctt atcgaaatgc taatccccac
     4261 caagaccgca ccgagggcca aagccaacga aaggtcgcct tggctcgttt gaagtgcgat
     4321 ggttgtggtc atggttctag tcaagccgcg aatgttgcca ccgaccaaga tcaccgcgcc
     4381 gacttccgaa acggtacgac caaagcccgc caagatggct gtcacaaggt ccgcgcgcac
     4441 gatcatgaga atttgtggcg cggcctgcaa tcgtgtcgca ccatcagcga tcagttcgtc
     4501 gccatagcgc gcccaagctc gttcgcttgc tcgatgcaca agcgctgtaa tgatgggaat
     4561 tgcgagtgtt gcttgtgcga taatcatcgc ggttggagtg aagagcagtg ccagtgagcc
     4621 gagaggtccg gaacgcgaga gggcgagata cagaacgagc ccaaccacga ccggcggcaa
     4681 cccgaaaaac gcattcatca acacgacaat caaccgccgc ccacaaaacg gatagattgc
     4741 gagggccgtc ccgatgggca agccgatagc cgctgcgagc gtcgtcgcgg ccaagctcac
     4801 gccgaccgaa agtccaatga tttgggtgaa gtcttgcaat tcgctattgg taatggacgg
     4861 tgccaggacg cgatattata acatgcggca agttgttgac aggccacgac attgagtttt
     4921 cggctcgcca tacgattctt tgtacgctcg aaatccgccc ggtaacgttg cggatgtctg
     4981 ctaacggcgt gcgccgtgca aaggcggcgc tttcgcgtcg tttcgccgcg cggcagaatt
     5041 tggtcgctat cggagtcata gcggacatcg agcgagctac accaatcgaa cgtgatttat
     5101 gagtatacgc ccagctgcat cacccctccg tccggcttga gccaccgggc tcgccggcat
     5161 cgcaggacgg ttccatcgcg tccgtttatt tgcgcccttg tgacccaggt gacagaatgc
     5221 tccgctggca tccgcgacaa tgataggcac tgccccctcg aaaaccaagc tgacggggac
     5281 cgacgatgac tgacctcggc gagatcaaac gcgaccggcc ctgcaatcga cgttggcttc
     5341 tccgccatgg agcgctggca ctcccgttca ttcttgtcgg cataccggcg cgcgccgtcg
     5401 agcgggtcgg ctcggtcgag gacgtcaagg gtgaggcatt cgccgagctc gaggcggttc
     5461 gccgcacgct cgatcgggcc gcaccggtct ttctcggcga cgaagtcgtg accggcgtcg
     5521 catcccgact gggaatgcgg ctcggtcgcg atacgacgat caggcttggc gaacaagcgc
     5581 ggctgaagat cgatcggttt cttgtcgatg ccggtgggga gatgacgctg cgctccgggc
     5641 cgttgctgtt cgatcgaccg ccgcgcaacg cacgcgcggg agtgcagatt cgcagcccct
     5701 tcgcgctgat cgcggtacgc ggcacgcaat tcttcgccgg gcccagcaac gagcgctttg
     5761 gcgtgttcgt cgcgcgcggc tcggtagcgg tgacctcggt gggtcagcaa gtaatcctgc
     5821 gcgagggcga aggtactgac tttgtctcgc ccggcacgcc gccgacgccg gtcaaacgat
     5881 gggcgccaca acgcatccgc gctgcgctcg caagcgtcag ctgaggcgcg agcgccactg
     5941 ctgctatatt ggcaaaacat gggccagcaa acgaagtgac cctttcactg ttgtcgcaaa
     6001 caagcctgtt cgccgcgatc gcacggcggt atctcgatgg gtaggatgcg ccgagcattg
     6061 cagcagcgcc tgccggttgc ggcgggaatc gcgacgctga ccgtttttct agcggcaggt
     6121 atcctgcttc ccaagaccac gagcgaggta ctgcgcgaca gcgccttcga catcgaactc
     6181 gccggcgatc aatggctgcg taggcccgca ccatccgacc tgaaggtgat cgtcgtcgac
     6241 atcgatcgtg cgtccatcga tgcgttgggc acctggccgt ggccgcggga gacgatggcg
     6301 cggatcgtcg aggcggtggc gaccgggcgg cccgcagcga tcgcgattga tgtgctgttc
     6361 gctgagcccg acgaccgctc gccggcggcg ctcgcacgcc ggcttggatc ggtgaccggc
     6421 cgcgccgaga tcagtacgct gggagaaagc ctgccggatg gtgacaagcg gctggcgcga
     6481 gccattggca gcgtccccgt cgcgctcggc tttgtgctcg atcccgatcg ccacggcaca
     6541 ttgccgggtg ccgcgatcgc gagccgcggg tccctgccat tcgacgattt gtggcaggcg
     6601 gcgggcgcga tcgggccgac gctgccgctc gcggcggcgg cgagcggact cggcgcgctc
     6661 tcgttacctg gtagcgccga tggagccatc cgcctggttc cggtcttcgt tgccgctggt
     6721 cgtgtcctga tgcctggtct agccgccgag gctctgcgcc tcgcatccgg tgcatcgagc
     6781 tatctcatca aaggcgaaga acccccgacg ctggtgatcg gcagccggcg ggttgcgctg
     6841 tcgcgcgatg ggctgctccg gctcgtgccg gtagcagcag ggcgtcgcgt cgcccgcacg
     6901 ctatccgcgg tcgacgtcct cgagggacgg gctgacggcg gccggctggc gggcgcgctg
     6961 gtgctgcttg gcggttccgc gccggagctc ggcggcttgc gcaagacacc cgcggacccg
     7021 ctgacgccct cggtgcaaat ccaggccgat gcggtc
//
LOCUS       DASWGM010000766         3510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_488532,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000766 DASWGM010000000
VERSION     DASWGM010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488532"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1718
                     /gene="glgA"
                     /locus_tag="VGA15_21160"
     CDS             303..1718
                     /gene="glgA"
                     /locus_tag="VGA15_21160"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX9590242.1"
                     /translation="MKVLFVTTEMDDFVRVGGLAAVSAALPRALRPWGDVRILLPGYR
                     DVVEQFLHIQIVGQCAALAEMPACSLGLASTKDGLPVYILLCPQLYDRPGNPYGDASG
                     RDWPDNDVRFGRFASAAAQLAAGTLDKNWAADLVHVNDWQAALVPAYLAWNGVQIPTI
                     LTIHNLAYQGLFPKESLRRIGAPEDSFHIDGLEFYDKLSFLKGGIVYASHLTTVSETY
                     AKEITTSELGCGLEGLLRRRSDAAQLTGILNGIDESWDPRVCAQLAQPFGAGDWEGKR
                     ANADYIRQQFGLALSRGPIFGLVARLVHQKGIDLVLSAADEIIEAGGQIIVTGSGEPA
                     IEQALVEAHRRRPDAIGVVIGFNEGQARRIFAGSDFTLMPSRFEPCGLSQMYAQRFGS
                     LPIGHQTGGLAETIMDGETGFLFAQPSTESFLGGIRRAFTTFVAKDRLDSMRRSAMSR
                     SFSWGVSAACYNALYRKAVSP"
     gene            complement(1866..3029)
                     /locus_tag="VGA15_21165"
     CDS             complement(1866..3029)
                     /locus_tag="VGA15_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9590243.1"
                     /translation="MNDVQDNKSPGDLGSLGRSLPGQVVLVLQGGGALGSYQAGVYQA
                     LHEAGIEPDWIIGTSIGAINASLIAGNTPQNRLSRLKQFWKRMEQNPIWSFRDVFPGF
                     NEKLSYWSTVTNGIPGFFRPNPLAHAGNDYPLGADRAGYYSTEPLEQTLRELVDFDLV
                     NRCAPRLTVGAAHVRTSQMRYFDSRDCELGVKHVMASGALPPAFPAIRIDGELYWDGG
                     ILSNTPTEAVFDDNPRKDSLIFAVHMWNPSGAEPTTMAEVLNRHKDVQYSSRIASHIA
                     RQQQAHRLRHVISQLAARLPEAERNSDVVRELAGYGCQTRMHVVRLLAPQLDMENHTK
                     DIDFSPSGIMQRWDAGYAHTRAVLDRSPWVGEFDPLSGVILHEQMEIMPVAAE"
     gene            3322..>3510
                     /locus_tag="VGA15_21170"
     CDS             3322..>3510
                     /locus_tag="VGA15_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9590244.1"
                     /translation="MEMHQVRYFLAVARVFNFTRAADECNVTQPSLTRAIKQLEAELG
                     GDLFRRERPAAQLTELGQR"
BASE COUNT          671 a         1086 c         1029 g          724 t
ORIGIN      
        1 ggccagcatc cgggtgcaat gcggaccggc gatcgtggtt gaaaaatcca gcacgcgtag
       61 gccctgaaaa ctctttgtca aaatcccctt atcgttgtcg gctatcgtca tcgcaaactc
      121 cctcggccgc gtaccttcag ccgcggcgag accctaaatt gcaccgttac cacagtgaag
      181 cgaatctctc gacgtcatct acttagactg tcgacgactc gccggctcgc cgctacattg
      241 caacgcagca aactggacct attcttgcat agttaaagtt agcgctggca cgagggcgtt
      301 tcttgaaagt cctgttcgtc accacggaaa tggatgactt tgtccgagtg ggcgggctcg
      361 cagccgtttc agcggccctc ccccgagcac ttcgcccttg gggtgacgtc agaattctcc
      421 ttcccggata tcgggatgtt gtcgaacaat tcctgcatat tcaaatcgtt ggtcaatgcg
      481 ctgcgctggc ggagatgccg gcatgttcac tcgggctcgc atcgaccaag gacggcctgc
      541 cggtttacat cctgctgtgt ccgcagcttt atgaccgccc cggcaatccc tacggcgatg
      601 cgtcgggccg ggactggccg gacaacgacg tgcggttcgg gcgatttgct tcagccgctg
      661 cccagttggc tgcaggcact ctggacaaga attgggcagc ggatctggtt cacgtcaatg
      721 actggcaggc agcactcgtg ccggcctacc tcgcctggaa cggcgtccag atcccgacca
      781 tcctgaccat ccacaatctg gcctatcaag gcctgttccc gaaggagtcg ctgcgacgga
      841 tcggtgcccc ggaggactcg ttccatatcg acggccttga gttctacgac aaattgtcct
      901 tcctcaaagg cggcatcgtc tacgcttccc atctgaccac cgtcagcgag acctatgcca
      961 aggaaatcac cacatcagaa ctcggctgcg gactagaagg tctgctccga cgccgctccg
     1021 atgccgcgca actgacgggg atattgaacg gcatcgacga aagctgggat ccaagggtgt
     1081 gcgcccagct ggcgcagccg tttggcgccg gggattggga aggcaagcgt gcgaacgcgg
     1141 actacatccg ccagcaattc ggtctggcgc tgtcccgcgg tccgattttc ggccttgttg
     1201 cccgtctcgt tcaccagaaa ggcatcgacc tcgtattatc agccgcggac gagatcatcg
     1261 aggccggcgg gcagatcatc gtgaccggca gcggtgaacc cgctatcgag caggcactgg
     1321 tcgaagcgca ccgccgcaga cccgatgcga tcggtgtcgt catcggtttc aacgagggcc
     1381 aagcccgcag gatttttgcc ggcagcgatt ttacgctgat gccgtcacgg ttcgaaccgt
     1441 gcggcctgag ccagatgtac gcgcagaggt tcggatcgct gccgatcggc caccagaccg
     1501 gcggtctggc ggaaaccatc atggacggtg aaaccggatt tctgttcgcc cagccgtcaa
     1561 ccgagtcgtt tctcggcggt atccggcgcg cattcacgac cttcgtcgcg aaggatcgcc
     1621 tcgactccat gcgccgcagc gcgatgtcgc gatcattcag ttggggcgtg tcggcagcct
     1681 gctacaacgc actttatcgg aaggccgtgt cgccttagat catgatccgg ttcggatcat
     1741 gatctaatct ctttgtttga gcatgatctc cgggcaaacg cttcgcgttt gtccagaggg
     1801 aaaaccggtt cccacccatc gggtcggggc tcgagggcat gcttttccgg atcatgctgc
     1861 aggcttcact ccgccgccac cggcatgatt tccatctgtt cgtgaagaat aactcccgag
     1921 agtggatcga actcgccgac ccagggcgaa cgatcgagaa ccgccctggt gtgcgcataa
     1981 cctgcgtccc agcgctgcat gatgccggat gggctgaaat cgatgtcctt ggtgtggttc
     2041 tccatatcca gttgaggtgc cagcaaccgc accacgtgca tccgggtctg acagccatat
     2101 ccagctagtt cccgcaccac gtcgctattg cgctccgcct cgggcagacg cgcggcaagt
     2161 tggctgatga cgtgacgcaa ccgatgcgcc tgctgctggc gcgcaatatg gcttgcgatc
     2221 cggctggaat actgcacgtc cttgtgccga ttaagcactt ccgccatcgt ggtgggctcg
     2281 gcgccggacg gattccacat gtgaaccgcg aagatcaggg aatccttgcg ggggttgtcg
     2341 tcgaacaccg cctcggtcgg ggtattcgac agaatgccgc catcccagta aagctcgcca
     2401 tcgatacgga tcgccgggaa tgccggcggt agcgcgcccg atgccatgac gtgcttgacg
     2461 ccaagttcgc aatcgcggct gtcaaaatat cgcatctggc tggtccggac atgcgcggcg
     2521 ccgaccgtca gccgcggcgc gcagcgattg accaggtcga aatcgaccag ttcccgcagc
     2581 gtctgctcga gcggctcggt cgaataatat ccggcccggt cggcaccaag cggatagtcg
     2641 ttgccggcat gggccagcgg attgggcctg aaaaaccccg gaattccgtt tgtcaccgtc
     2701 gaccagtaag atagtttttc attgaagccg ggaaatacat cgcgaaaact ccagatcggg
     2761 ttctgctcca tccgcttcca gaactgcttt agccgcgaca ggcgattttg cggcgtgttg
     2821 ccggcgatca gactggcatt gatggcgccg atcgatgtac cgatgatcca gtccggctct
     2881 ataccggcct cgtgcagcgc ctgatagacg ccggcctgat aagatcccag cgcgccaccg
     2941 ccctgcagca cgagcacgac ctgaccggga agcgacctgc ccagtgaacc caagtcccct
     3001 ggagatttgt tgtcttgaac gtcgttcatg tcacgctccc gttggatgcc tgttgcgttc
     3061 tctgatgttc gcgaaagatt cgaatttgtt acgctgacca acgtgtttca gtgcgcggcc
     3121 cggccgccat caatccgcag ggcgtgccgg tgatttcacc gcctcgcgga gccgttgatc
     3181 tctgctgaaa cctgcacggc tgaggggatt tacggaaata cccattagct tccgaaacga
     3241 taggccggcg ctatcgcggt tccagtgtcc ttgccgggcg ttatcggcta ccgtgccagc
     3301 acgatccgac cgggcacaat catggaaatg catcaggttc gctacttcct ggcggtcgcg
     3361 cgtgtgttta acttcacgcg cgccgccgac gagtgcaacg tgacccagcc gtcactgaca
     3421 cgggcgatca agcagctcga ggcggaactc ggcggcgatc tgttccggcg cgagcggccg
     3481 gcggcgcaat tgaccgaact tggccagcgc
//
LOCUS       DASWGM010000767         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1884300,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000767 DASWGM010000000
VERSION     DASWGM010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884300"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /gene="atpD"
                     /locus_tag="VGA15_21175"
     CDS             complement(<1..977)
                     /gene="atpD"
                     /locus_tag="VGA15_21175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX9590245.1"
                     /translation="MATPANQTGRITQVIGAVVDVQFEGHLPAILNALETQNGGNRLV
                     LEVAQHLGESTVRTIAMDTAEGLVRGQEVGDTGNPIMVPVGVGTLGRIMNVIGEPVDE
                     AGPIKSEGNRAIHQPAPAYTDQSTESEILVTGIKVVDLLAPYARGGKIGLFGGAGVGK
                     TVLIQELINNVAKAHGGYSVFAGVGERTREGNDLYHEFIESGVNKKGGGEGSKCALVY
                     GQMNEPPGARARVGLTGLTVAEHFRDQGQDVLFFVDNIFRFTQAGSEVSALLGRIPSA
                     VGYQPTLATDMGAMQERITTTTKGSITSIQAIYVPADDYTDPAPATTFA"
     gene            complement(1015..1893)
                     /locus_tag="VGA15_21180"
     CDS             complement(1015..1893)
                     /locus_tag="VGA15_21180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX9590246.1"
                     /translation="MASLKDMRVRIASTKATQKITKAMQMVAASKLRRAQNAAEAARP
                     YAEKMDAVISNIAGAAAGSPGAPVLMAGTGNDKVHLLLVCTGERGLSGAFNSSIVRLA
                     REHALSLINEGKEVKFFCVGRKGLEQLRRNFEKQIVENVELRSVRQLGFVNAEDIAKK
                     VIARFEAGEFDVCTLFYSRFKSVIAQVPTAQQIIPLVVAAPAANAGPATAYEYEPEED
                     EILSGLLPRNLAVQIFRALLENNASFYGAQMSAMDNATRNAGDMIRKQTLIYNRTRQA
                     MITKELIEIISGAEAL"
     gene            complement(2073..>3216)
                     /gene="atpA"
                     /locus_tag="VGA15_21185"
     CDS             complement(2073..>3216)
                     /gene="atpA"
                     /locus_tag="VGA15_21185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX9590247.1"
                     /translation="DVKAPGIIPRKSVNEPMATGLKAIDALIPIGRGQRELIIGDRQT
                     GKTAIALDTILNQKPLNAQTDEKIKLYCIYVAIGQKRSTVAQFVKVLEEQGALEYSII
                     VAATASDPAPMQYIAPFTGCTMGEYFRDNGMHAVIIYDDLSKQAVAYRQMSLLLRRPP
                     GREAYPGDVFYLHSRLLERAAKLNDEHGSGSLTALPVIETQANDVSAYIPTNVISITD
                     GQIYLESDLFFQGIRPALNVGLSVSRVGSSAQTKAMKKVAGKIKGELAQYREMAAFAQ
                     FGSDLDASTQRLLARGSRLTELLKQPQFSPLKMEEQVVVIYAGVNGYLDGIPVAKVRA
                     FEDGLLSLLRGKNVDILNTIRDSRDLGDDTAAKLKSVVEGYTKTFA"
BASE COUNT          554 a          987 c         1031 g          644 t
ORIGIN      
        1 tgggcaaacg tcgtcgctgg cgccggatcg gtgtagtcat ccgcggggac gtaaatcgcc
       61 tgaatcgacg tgatcgagcc cttggtggtg gtggtgatgc gctcttgcat cgcgcccatg
      121 tcggtggcaa gcgtcggctg atagcccacg gcgctgggaa tacgtcccaa cagcgccgac
      181 acttccgaac ccgcctgggt aaagcggaag atgttgtcga cgaagaacag cacgtcctgg
      241 ccctgatcgc ggaagtgttc ggcgacggtg agaccggtga ggccgacgcg ggcgcgggcg
      301 cccggcggct cgttcatctg gccatagacc agcgcgcatt tggaaccttc gccgccgccc
      361 ttcttgttga cgccggattc gatgaactcg tgatagaggt cgttgccttc gcgggttcgc
      421 tcgccgacac cggcgaacac cgaataaccg ccgtgggctt tcgcaacgtt gttgatcagt
      481 tcctgaatca gcacggtctt gccgacgccg gcgccgccga acaggccgat cttgccaccc
      541 ctggcgtagg gcgccaggag atcgacgacc ttgatgccgg tgacgagaat ttccgattcg
      601 gtggattggt cggtgtaggc cggcgctggc tgatggatgg cgcggttgcc ttccgacttg
      661 atcggcccgg cttcatcgac cggttcgccg atgacgttca tgatgcgccc gagcgtgccc
      721 acaccaaccg gcaccatgat cgggttgccg gtgtcgccga cttcctgacc gcggaccagg
      781 ccttcggccg tatccatggc gatggtgcgc acggtggatt cgccgagatg ctgcgcgacc
      841 tcgagcacca ggcggttgcc gccgttctgg gtctcgagtg cgttcagaat ggccggcaga
      901 tgtccttcga actgcacgtc gacgacggcg ccgatgactt gcgtgatgcg tccggtctgg
      961 ttggcgggtg tagccatgaa atgtctctcc ttcgaaattc tgtcttagcg gctgctagag
     1021 cgcctcggcg ccggagatga tttcgatcag ctccttggtg atcatcgcct ggcgggttcg
     1081 attgtagatc agggtctgct tgcggatcat gtcgccggcg ttgcgggtgg cgttgtccat
     1141 cgcgctcatc tgcgcgccat agaacgaggc gttattttcc aaaagcgcac gaaaaatctg
     1201 caccgcgaga ttgcgcggca gcaggccgga cagaatctca tcttcttccg gctcgtattc
     1261 ataggccgta gccgggccgg cattggcagc gggggctgcc acgaccagcg gaatgatctg
     1321 ctgcgcggtc gggacctgcg cgatcacaga cttgaaccgc gaatagaaca gcgtgcagac
     1381 atcgaactcg ccggcctcga agcgcgcgat gactttcttc gcgatgtctt cggcgttgac
     1441 gaagccaagc tgcctcaccg agcgcagctc gacgttttcg acgatctgct tctcgaagtt
     1501 gcgccgcagc tgctcaagac ccttgcggcc gacgcagaag aatttaactt ctttgccttc
     1561 attgatcagc gacagcgcgt gctcgcgggc gagacgcacg atcgaagaat tgaatgcccc
     1621 ggacaggccg cgctcgccgg tgcaaaccag caagagatgc accttgtcat tgccggtccc
     1681 cgccatcagc accggcgcgc cgggcgaacc cgcggctgca ccggcaatat tcgaaatcac
     1741 cgcgtccatt ttttcggcgt aggggcgcgc cgcctcggcg gcgttctgcg cgcggcgcag
     1801 cttcgaggcg gcgaccatct gcatggcctt ggtgatcttc tgcgtcgcct tggtggaggc
     1861 gatgcggacc cgcatgtctt tcaagctggc cattgcggct gacttatcct ctagctcgac
     1921 cccgttccct ccccccttgc gggggagggt tagggagggg ggtgagcaac aacctcagag
     1981 cttgttgctc acccccctcc cgaccggctt cgccggtcga cctcccccgc aagggggaga
     2041 taactaaaga gagggctttc gcccgcgtca aattaagcaa atgtcttggt gtaaccctcg
     2101 accacgctct tcagcttggc ggcggtatcg tcgccgagat cgcggctgtc gcggatggtg
     2161 ttgaggatat cgacgttctt gccgcgcagc agcgacaaca ggccgtcctc gaaggcgcgc
     2221 accttggcga ccgggatgcc gtcgagatag ccgttgacgc cggcatagat caccacgacc
     2281 tgctcttcca tcttcagcgg cgagaactgc ggctgcttca agagttcggt gaggcgcgaa
     2341 ccgcgcgcca aaaggcgctg cgtcgaggcg tcgagatcgg agccgaactg cgcgaacgcc
     2401 gccatttcgc ggtactgcgc aagctcgccc ttgatcttgc cggcgacctt cttcatcgcc
     2461 ttggtctgcg ccgacgatcc gacgcgcgac accgacaggc cgacgttcag cgccggacga
     2521 atgccctgga agaacagatc gctctcgagg tagatctggc cgtcggtaat cgagatcacg
     2581 ttggtcggga tgtaggccga cacgtcattg gcctgggtct cgatcaccgg caacgccgtc
     2641 agcgagcctg agccgtgctc gtcgttcaat ttggcggcac gctcaagcaa gcgcgaatgc
     2701 agatagaaca cgtcgcccgg ataggcttcg cggcccggcg ggcggcgcag cagcagagac
     2761 atctggcggt aggccaccgc ctgtttcgac agatcgtcgt agatgatgac ggcatgcatg
     2821 ccgttatcgc ggaagtattc gcccatggtg cagccggtga acggcgcgat gtactgcatc
     2881 ggcgccgggt cggaagcggt ggctgcgacg atgatcgaat attccagcgc gccctgctct
     2941 tccagcacct tgacgaattg cgccacggtc gagcgcttct ggccgatcgc gacatagatg
     3001 cagtacagct tgatcttctc gtcggtctgc gcgttgagcg gcttctggtt gagaatggtg
     3061 tcgagcgcga tcgcagtctt gccggtctgg cggtcgccga tgatcaattc gcgctggcca
     3121 cggccgatcg ggatcagggc atcgatcgcc ttcagcccgg tcgccatcgg ctcgttgacc
     3181 gatttgcgcg gaatgatgcc gggcgccttg acgtcg
//
LOCUS       DASWGM010000768         4905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1451732,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000768 DASWGM010000000
VERSION     DASWGM010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451732"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2588)
                     /locus_tag="VGA15_21190"
     CDS             complement(<1..2588)
                     /locus_tag="VGA15_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HEX9590248.1"
                     /translation="MSGATPDPSEPARDADAASRLQLTQALQRAKYAIAWERSWPHFA
                     RLLTVAGLFLAVSWAGLWLALPFVARAIGLGVFVLLALGALLPLVRFRWPSREEGLSR
                     LDRGTGIRHRPATALTDTLATQDPVALALWQAQRERTLASIKRIRAGLPSPRLVIHDP
                     WALRALVAVLMVAAYIAAGDERTLRTGAAFDWNGVLSPANIRVDAWVTPPLYTGKPPI
                     ILSAANKDAAMPAAGPLPVPAGSTLIVRSSGGTLDVVAAGSVTEAVPTEQAPKGTNER
                     HFTISGDGTAHVRAPSGQPQWKFSAIPDRAPTIALAKDPERQARGSLQMSYKIEDDYG
                     VTEAQAHFSVRPGDAPKAGTEPRPLFAPPQFSLVLPNARTRNGVGQTVKDLSEDPYAG
                     ADVSLTLTAKDEAGNEGRSEPFNMRLPERLFTKPLARALIEQRRNLALDANQNTQAYA
                     ALDALMIAPELFMPETGQYLGLYSVARQLEAARTDDAMREVVASLWALAVTIEDGDIT
                     DVDKALRAAQDALKQALERGASDEEIKKLTENLRAALDNFIRQLAEQQRNNPQQLARP
                     LDPNTKMLSQQDLKNMIDRMERLSRSGDKDAAKQLLDQLQQMLENLQMAQPGQSGDSD
                     MEQALNELGDMIRKQQQLRDKTFKQGQDSRRDRMRGKQGDQSMSDLQQDQQGLRDRLK
                     RLQEELAKRGMGQGQRGEKGQRGEKGQRGEQGQQGQEGQQGDQGDGEDGLGEADSAMD
                     DATGRLGEGNADGAVDSQGRALEALRKGAQSLAEAMQQGDGDGQGDSPGNRAGRQQNG
                     ANSNDPLGRPLHGREYGDDLTVKIPGEIDVQRVRRILEELRRRLADPQRPQIELDYIE
                     RLLKDY"
     gene            2760..3140
                     /locus_tag="VGA15_21195"
     CDS             2760..3140
                     /locus_tag="VGA15_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9590249.1"
                     /translation="MDKKTHDKGLEIRKAVLGEAYVSNALKNADDFNKPFQELVTEYC
                     WGAVWGREELPRKTRSMLNLAMISILNRPHELRVHIKGALVNGVTKDEIREIFMQVAI
                     YAGVPAGVDSFRIAREVFAEIDKG"
     gene            3168..4076
                     /locus_tag="VGA15_21200"
     CDS             3168..4076
                     /locus_tag="VGA15_21200"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590250.1"
                     /translation="MDIGFIGLGKMGFPMARRLIEAKHRLVVFDQRQEAVDKLVALGA
                     QAASSPKDVADRAETVLASLPSLQASLEVATGSGGVIEGKRVRRFVDLSTVGSHMAVR
                     IHDLLAKRNIVQLDSPVSGGVGGAEKGTLAVMVSGPKAEFELIKPALGVIGKVFFIGE
                     KPGSAQTMKLANNLLSATAVVATSEAVVMGVKSGLDPHVMIDVINAGSGMNTASRDKF
                     PRAILPRTFDFGFATGLMVKDVRLCLEEAKSLGLSMEVAEAVGRLWEVVIRQMGAESD
                     FTSAIKPIEKAAGVMVGGGTGAHAAK"
     gene            4225..4539
                     /locus_tag="VGA15_21205"
     CDS             4225..4539
                     /locus_tag="VGA15_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9590251.1"
                     /translation="MTRSSKSPFGPTNPRTRCPKPGRWLLQDAKARFSELVRRVRSEG
                     PQRVTVHGRDEVVVISAEEFRRLKGDLTGESLIAAMQALPHRDVNIEPRRAPMPVREI
                     TQ"
     gene            complement(4774..>4905)
                     /locus_tag="VGA15_21210"
     CDS             complement(4774..>4905)
                     /locus_tag="VGA15_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX9590252.1"
                     /translation="QSSSYNTRALVPEVLVKADQYAVVRPRIEVDALIAMDKPAPWL"
BASE COUNT          838 a         1616 c         1554 g          897 t
ORIGIN      
        1 taataatcct tcagcagccg ctcgatgtaa tcgagctcga tctggggccg ctgcggatcg
       61 gcgaggcggc ggcgcagttc ttccagaatc cggcgcaccc gctggacatc gatctcgccg
      121 ggaatcttca ccgtcagatc gtcaccgtat tcgcgcccat gcagcggccg cccgagcgga
      181 tcgttgctgt tggcgccgtt ctgctgtctg cctgcgcgat tgcccgggct gtcgccctga
      241 ccgtcgccgt caccctgctg catcgcctcc gccagactct gggcgccctt gcgcagcgct
      301 tccagcgccc ggccctggga atccacggcg ccatcggcgt tgccctcccc gagcctgccg
      361 gtggcatcgt ccatcgccga atcggcttcg ccgagaccgt cctcaccgtc gccctggtcg
      421 ccttgctggc cttcctggcc ctgctggcct tgctcgccgc gttggccttt ctcgccgcgc
      481 tggcctttct caccgcgctg gccctgcccc atgccgcgct tggccagctc ttcctgcaat
      541 ctcttgagcc ggtcgcgcag gccctgctgg tcctgctgca gatcgctcat gctttgatcg
      601 ccctgcttgc cgcgcatgcg gtcgcgccgc gaatcctggc cctgtttgaa ggtcttgtcg
      661 cgcaactgct gctgcttgcg gatcatgtcg ccgagctcgt tcagcgcctg ttccatgtcg
      721 ctgtcgccgg actggccggg ctgcgccatt tgcaggtttt ccagcatctg ctgcagctgg
      781 tcgagcaatt gcttggccgc atccttgtcg ccggagcgcg acaggcgctc catgcggtcg
      841 atcatgttct tgaggtcctg ctggctcaac attttggtat tgggatccag cggccgcgcc
      901 agttgctgcg gattgttgcg ctgctgttcg gcgagttgac ggatgaaatt atccagcgcc
      961 gcgcgcaaat tctcggtgag cttcttgatc tcttcgtcgc tggcgccgcg ttccagcgcc
     1021 tgcttgagcg cgtcctgcgc ggcacgcaat gccttgtcca cgtcggtgat gtcgccatcc
     1081 tcgatggtga cggcgagcgc ccacaggctg gcgacgacct cgcgcatggc gtcgtcggtg
     1141 cgggcggcct ccagctgcct tgcgacgctg tacagcccga gatactggcc ggtttcgggc
     1201 atgaacaatt cgggtgcgat catcagcgca tccagcgccg cataggcttg cgtgttttga
     1261 ttggcgtcga gcgcgagatt gcggcgctgc tcgatcagcg cccgcgccaa gggcttggtg
     1321 aacaaccgct ccggcagccg catgttgaac ggctcgctgc ggccctcatt gccggcctcg
     1381 tccttggccg tcagcgtcaa gctgacgtcg gcgccggcat aaggatcctc gctgagatcc
     1441 ttgacggtct ggccgacgcc attgcgcgtt cgcgcattcg gcagcaccag cgagaattgc
     1501 ggcggcgcga acagcggccg cggctccgta ccggctttcg gcgcgtcgcc gggccggact
     1561 gaaaaatgcg cctgcgcttc ggtgacgccg taatcatcct cgatcttgta ggacatctgc
     1621 agcgagccgc gggcctgacg ctcgggatct ttcgcaagcg caatggtcgg agcgcggtcg
     1681 ggaatggcgc tgaacttcca ttgcggctgc ccggacggcg cgcggacatg tgccgtacca
     1741 tcgcccgata tcgtgaaatg ccgttcgttg gtgcccttgg gcgcctgctc ggtcggcacc
     1801 gcttcggtca cgctgcctgc ggcgacgaca tcgagcgtac cgccgcttga gcgcacgatc
     1861 agcgtgctgc ccgccgggac cggcaacggt cccgcggccg gcatcgccgc gtccttgttc
     1921 gccgccgaca gaatgatcgg aggcttgccg gtgtagagcg gcggcgtcac ccaggcgtca
     1981 accctgatat tggccggcga cagcacgccg ttccagtcga acgccgcgcc ggtgcgcagc
     2041 gtgcgctcgt cgcccgcggc aatataggcc gcaaccatca gcaccgcgac cagcgcgcgt
     2101 aacgcccagg gatcgtggat cacaagccgc ggcgacggca gaccggcgcg gatccgcttg
     2161 atcgacgcca aagtgcgctc gcgctgcgcc tgccacagcg ccagcgcgac cggatcctgc
     2221 gttgcaagcg tatcggtgag cgcggttgcc gggcgatggc ggatgccagt gccgcggtcc
     2281 agccggctca gcccctcttc gcggctgggc cagcggaacc ggaccaacgg aagcagcgcc
     2341 ccgagtgcca aaagcacgaa cacaccaagg ccgatggcgc gcgcgacaaa cggcagcgcc
     2401 agccacaatc cggcccagga taccgccaga aacagccccg cgaccgtcaa caggcgcgcg
     2461 aaatgcggcc agcttcgctc ccatgcgatg gcgtattttg cccgctgcaa ggcctgcgtc
     2521 agctgcagcc tggaggcagc atcagcgtcg cgtgctggct ctgaggggtc gggggtggcg
     2581 ccgctcaatc aactctcctg gttgcccgaa acagcaccct agcacaacgg cggcaatgag
     2641 gcacgtctcg caatgcacaa cccacacctc aaatcgcgca taacacgaag gcgtgacgcc
     2701 agcgccccga cctcgtaacg tccggtgtca tcccaccagt aagaccaaaa ggaagcgcaa
     2761 tggacaagaa aacccacgat aagggactgg aaatacgcaa agccgtgctc ggcgaggcct
     2821 acgtttcgaa cgccctgaag aatgccgacg atttcaacaa gccattccag gaactggtca
     2881 ccgaatattg ctggggcgcg gtgtggggcc gggaggaact gccgcgcaag acccgcagca
     2941 tgctcaatct ggcgatgatc tcgatcctca accggccgca tgaattgcgg gtgcacatca
     3001 agggcgcgct ggtcaacggc gtgaccaagg acgagatccg cgaaatcttc atgcaggtgg
     3061 cgatctatgc cggcgtcccc gcgggcgtcg acagttttcg gattgcgcgc gaagtgttcg
     3121 ctgaaatcga caagggctaa atcgacttgg gctaaaggcg tatcatcatg gatatcggat
     3181 tcatcggcct cgggaaaatg ggctttccca tggcgcgccg cctgatcgag gcaaaacatc
     3241 ggctcgtggt cttcgaccag cgccaggagg cggtggacaa gctggtcgcg ctgggcgcgc
     3301 aagccgcatc gtcaccgaaa gacgtcgccg accgcgccga gaccgtgctg gcgagcctgc
     3361 cgtcgctgca ggcctcgctc gaggtcgcaa ccggcagcgg cggcgtcatc gaaggcaagc
     3421 gcgtcaggcg cttcgtcgat ctctccaccg tcggatcgca catggcggtg cgaatccacg
     3481 atttgctggc gaaacgcaac atcgtgcagc tcgacagtcc cgtcagcggc ggcgtcggcg
     3541 gcgccgaaaa gggcacgctc gccgtcatgg tgtcgggccc gaaagcggaa tttgaactga
     3601 taaaacccgc gctcggcgtg atcggcaagg tgttcttcat cggcgagaaa cccggttcgg
     3661 cgcagaccat gaagctcgcc aacaatctgt tgtcggctac cgccgtcgtc gccacctccg
     3721 aggccgtggt gatgggcgtc aaatcgggcc tcgatcccca tgtcatgatc gacgtcatca
     3781 atgcgggatc cggcatgaac accgcgagcc gcgacaagtt tccgcgcgcg atcctgccgc
     3841 gcaccttcga tttcggcttt gccaccgggc tgatggtgaa ggacgtccgg ctgtgcctcg
     3901 aagaagccaa gtcgctcggg ctatcgatgg aggtcgccga agcggtcgga cggctatggg
     3961 aagtggtgat tcgccagatg ggcgcggagt ccgatttcac ctcggcgatc aagccgatcg
     4021 aaaaagccgc gggcgtgatg gtgggtgggg gaaccggcgc tcacgccgcc aagtagtggc
     4081 gcgcaccgcc aatgctgcgc acggcactca ctcctctctc ccttgtgtag ggaggaaagg
     4141 atcgacgggc ttcactgctt cgtttactct ccccggacag cgaaccattg aagttccccg
     4201 gaccatggtg atgtactggt cactatgacg agaagttcaa aatctccgtt cggaccgacg
     4261 aacccacgca cccgctgccc gaaacccggc cgctggctgt tgcaggacgc aaaggcgcgc
     4321 ttcagtgagc ttgtgcggcg cgtccgcagt gagggaccgc aacgggtgac cgtgcacgga
     4381 cgcgacgaag ttgtcgttat ctcagccgag gaattccgtc gtctcaaagg cgatctcacc
     4441 ggcgaatcct tgatcgccgc aatgcaggcc ttgccgcatc gcgacgtcaa tatcgaaccg
     4501 aggcgcgcgc ccatgcccgt gcgcgagatc acccagtgag gtgatttgcg aatgtgcgtg
     4561 gaatccgatt tcacctcgac gatcaagcca aacgacggac gtgccccgga tgctgcgcag
     4621 cgccatcagc gcgtttacgc gcgtctttga cgcgccatgc cttgcggcgt ggtgcgctgc
     4681 tgaatccggg gtccatcttt ttagctagaa catgggtccc ggctctgcgg agcagcgcga
     4741 agtgcgctgc accgcgtccg ggacacgagg tattcacagc cacggcgcgg gcttgtccat
     4801 cgcgatcagc gcatcgacct cgatgcgggg ccgcaccacc gcgtattgat cggccttgac
     4861 cagcacttcc ggcaccaggg cgcgggtgtt gtagctcgag gattg
//
LOCUS       DASWGM010000769         3365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1588599,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000769 DASWGM010000000
VERSION     DASWGM010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588599"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..525)
                     /locus_tag="VGA15_21215"
     CDS             complement(349..525)
                     /locus_tag="VGA15_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590253.1"
                     /translation="MIEGRISVLMRGRGLKRCRAEGAERFALFVGAAVLVNNLMIVAA
                     LLTKRSSRRSKAAR"
     gene            1043..3118
                     /locus_tag="VGA15_21220"
     CDS             1043..3118
                     /locus_tag="VGA15_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX9590254.1"
                     /translation="MTTHEALLPTTVLRRKAIVYVRQSTPQQVQSNLESQRRQYDLVD
                     VARRRGFTDVEIIDDDLGRSASGAVDRPGFDRLVAALCAGQVGAVLCLDASRLARNGR
                     DWHHLLELCGLVDARVIDLDGVYDPCRPNDRLLLGMKGSISEFELGIIRSRMYDAARS
                     KAKRGELRISVPIGYTWDRRVGLGLDPDRRLQEVIRLVFQKFRELGSARQVLLWMTSQ
                     NIHFPYPSNGRTLTSFEWRAIRYRNVISILKNSFYAGVYAYGKSETHASIVDGRARKS
                     YGHHKPMDAWEVFIKDHHEGYISWVEYERNQALLAGNNYGRVGDTKSGRGGRALLGGL
                     ICCARCGRRLSVAYTGRNTLPVYRCDKLNVPPGQRRHCFSFGGKRIDGIVAAEILRAV
                     APMAIEAAEEAERMLRDEDQDRRRIAELELQQAQYEASLAERRYAACDPDNRLITAQL
                     EKAWEDALQRVEASRQRLDALREPDAGDACPDLSGLADDLSAAWKAPRTTMRTRQRLV
                     RALITEIIADVDEAAGEIVLVIHWKGGQHSELRVRKLRTGEHGCNTPEQALAVMRSMA
                     GRWSDEHIAASLNRMGMPTGQGKTWTADRIGSLRRVHGIHCCLSADKNGEWLTLSDAA
                     AKLGVSHHQVRKLIKAGILTSQQIMPDAPHQIRAADLSSEQVAAALKRKGRPCRLNSE
                     KQISLFSDT"
     gene            complement(3107..>3365)
                     /locus_tag="VGA15_21225"
     CDS             complement(3107..>3365)
                     /locus_tag="VGA15_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590255.1"
                     /translation="APSLKRHRRAFRRAPELYGADRGFFSEQNVAACVQGAVKTVCIP
                     QRGGSKTPQRQAYERSAAFKQGQRFRAGMMTDSFCTSSSSI"
BASE COUNT          639 a         1001 c         1086 g          639 t
ORIGIN      
        1 attcagtctg tgcgcgaatc atctctgata tgtgttacac cacacatcac agtggcaagc
       61 aaaacttgca ccgaaaaaac gctagaaatc gctattccac cgcaatatct tgcctttcgc
      121 gacagaaact agtctagtct agtttctgtc gcgaaatgcg tggattctaa caagcatagt
      181 tcagttatgc cgatgaactg ggcctaagct aaccatggtt tgcgtaaatt tgcgagcgct
      241 gtacgcgcgg ctttctgtat tgattcgctg tcgccaggta gagttgcggt tggcgacgtg
      301 gtgaggcgcg gcggttttgt tccacctgtg cccacgattg gtgctggttt atcgggcggc
      361 ttttgatcgg cgcgaggagc gtttggtcag cagcgcggca acgatcatga ggttgttgac
      421 gagcaccgct gcgccgacga acagcgcgaa gcgctcggcg ccttcagcac ggcaacgctt
      481 caagccgcgc ccgcgcatca gcaccgagat gcgcccctcg atcatctcgc ggtcattctg
      541 cccatatcgg gcgggatgtc gttatccact atcgctggca tcctttgtac ggacgaaccg
      601 cgcgtcgcgt tcagattgag cggcgcgcga ccggcgattt ggtgcacgtc gagcacacgt
      661 cgggcgtcgt gacgatcctg ccggcgtgga agctcgatgc ggtctattgc gccagcctca
      721 aggtcggtgc accgcgggtg tcattggcgg cgctatgcgc attgcatgag gtgctgatcg
      781 cctgcgaatc gcggcaggtc tccgcggacg gcaacatcgt cacgcaggag acgcaggatg
      841 ggatcgcagc taccgctcgc gcgaaggacg aagcttgctc tgaggccgga acccgccccc
      901 ccgacattac cccgccagct cgatctcgtt ctcgacgacg tgcggctttg gggcatggct
      961 ccgccggaac gccaagcggt tctcaggtca ctggctcgcc tgctgctcga ggcaagcggc
     1021 gtcgcgacac gggaggccgg cgatgaccac ccatgaggcg cttctcccga caaccgtgct
     1081 gcggcgcaag gcgatcgtct atgtccgcca atcgacgccc cagcaggtgc aatcgaacct
     1141 cgagagccag cggcgccagt acgaccttgt cgacgtcgca aggcggcgtg gcttcactga
     1201 cgtcgagatc atcgacgacg atcttgggcg gtctgcgagt ggcgctgtcg accgaccagg
     1261 cttcgaccgc ttggtcgcgg ctctgtgcgc cgggcaggtc ggagctgtgc tctgcctcga
     1321 tgcctcgcgg ctcgctcgca atggccgcga ctggcatcat ctcctggaat tgtgtggatt
     1381 ggtggatgcg cgtgtcatcg acctcgatgg cgtgtacgat ccgtgccggc cgaacgaccg
     1441 cctgctgctt gggatgaagg gcagcatcag cgagttcgag ctggggatca tccgctcccg
     1501 gatgtacgac gcggcacgat cgaaagcgaa gcgcggcgag ctgaggatct cggtgccgat
     1561 cggctatacc tgggatcggc gcgtcggcct cggcctcgat ccagatcgac ggctgcagga
     1621 ggtgatccgg ctggtgttcc agaagttccg ggagcttggc agcgctcgcc aagtgctgtt
     1681 gtggatgacg tcgcaaaaca tccactttcc ctatccgtcg aacggccgga cattgacgtc
     1741 tttcgagtgg cgggcgatca ggtatcgcaa tgtcatctcg attctcaaaa actccttcta
     1801 cgctggcgtc tacgcgtacg ggaagagtga gacgcacgca tcgattgtcg acggccgcgc
     1861 gcgcaagagc tatggccacc acaagccgat ggacgcgtgg gaggtgttca tcaaggatca
     1921 tcacgagggt tacatcagct gggtggagta tgagcgcaat caggcgctgc ttgccggcaa
     1981 taactacggc agggttggtg acacgaagtc tggacggggc gggcgcgccc tgctgggcgg
     2041 tctcatctgt tgcgcacgct gtggccgtcg gctcagcgtt gcctacaccg gccgcaatac
     2101 cctgccagtt tacaggtgcg acaagctgaa cgtgccgccg gggcagcggc ggcattgctt
     2161 cagcttcggc ggcaagcgca tcgacgggat agtcgcggcc gagatattgc gcgccgttgc
     2221 gccgatggcg attgaggcgg ctgaggaggc cgagcgcatg ttgagagacg aggaccagga
     2281 ccgacggcgc atcgccgagc tggagctgca acaggcccag tacgaagcat cgttggcaga
     2341 gcgtcgctat gccgcttgcg accccgacaa tcgcctgatt acagcgcaac tggagaaggc
     2401 gtgggaggat gcgctgcagc gtgtagaggc gtcccgacag cggctcgacg cactgcgcga
     2461 gcccgatgcc ggcgatgctt gtcccgatct ctcgggactg gctgatgacc tctcggcggc
     2521 atggaaggcg ccaaggacaa caatgcgaac gcgccaacga ttggtgcggg cgttgatcac
     2581 cgagatcatt gccgacgtcg acgaggcggc gggagagatc gtgcttgtca tccactggaa
     2641 aggcggccag cattccgagc tgcgcgtccg caagctgcga accggcgagc atggctgtaa
     2701 cacacccgag caggcgctcg ccgtcatgcg cagcatggcc ggccgatggt cggatgagca
     2761 tattgcagca tctctgaacc gaatgggaat gccgaccggc caaggcaaga cctggacagc
     2821 ggaccgtatc gggtcgctgc gccgcgtgca tggcattcac tgctgcctct ccgccgacaa
     2881 gaacggcgag tggctgacac tcagtgacgc ggctgcaaag cttggtgtca gccatcatca
     2941 agttcgcaag ctcatcaagg ccggaattct gaccagccag cagatcatgc ctgacgcacc
     3001 tcaccagatc cgtgccgccg acctgtcaag cgaacaggtc gctgccgccc tcaaacgaaa
     3061 aggtcgcccg tgtcgactaa attccgaaaa acagatctca ctgttttcag atacttgaag
     3121 aggaggtgca gaatgaatca gtcatcatgc cggcgcgaaa gcgctggcct tgcttgaacg
     3181 ccgccgatct ttcgtaggcc tggcgttgcg gcgttttgct gccgccgcgc tggggaatac
     3241 ataccgtctt gacggcgcct tgcacgcacg ccgctacgtt ttgctcgctg aagaagccgc
     3301 gatctgcgcc atagagttcc ggggcacggc ggaacgcgcg gcggtggcgc ttgagcgacg
     3361 gcgcc
//
LOCUS       DASWGM010000770         6650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2696588,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000770 DASWGM010000000
VERSION     DASWGM010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2696588"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGA15_21230"
     CDS             <1..283
                     /locus_tag="VGA15_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9590256.1"
                     /translation="ASLSPTALARLINAVPIELNGVAPIARAISTAGGIAFSELDADF
                     MIHRLPGIFAAGEMLDWEAPTGGYLLQASFATGAAAGRGVLKWLNRRIG"
     gene            481..729
                     /locus_tag="VGA15_21235"
     CDS             481..729
                     /locus_tag="VGA15_21235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590257.1"
                     /translation="MILKPLMAAVAMAAAVSAMSVVVPSSAYAQSGAEAEIIGFHDLC
                     NKGDRRACVRFGILIGQNQQRHADWRRAHADWWWWEKQ"
     gene            complement(775..1857)
                     /locus_tag="VGA15_21240"
     CDS             complement(775..1857)
                     /locus_tag="VGA15_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HEX9590258.1"
                     /translation="MTTPLAAKIAREYGTPAAVIDMDRVERNIARVQAACDAAGVANR
                     PHIKTHKSPLLAKLQIAAGARGITCQKLGEAEVMAEAGIDDILISYNLIGDEKMARLG
                     ALQARTNMTVAADNSVVIAGLPQAAAASGRPLSVVVECDTGRKRAGVETPAEAIALAR
                     QIAASKGLTFAGFMLYPTETGWGDAQRFYDEALAGIREHGLDAAIVSTGGTPNLKNVG
                     KLKGATEHRPGTYIYNDRMQVAAGVATWDDCALNIYSTVVSRAGPDRGILDAGSKTLT
                     SDTGGGLDGHGLILEHPEAKIARFAEEHGFLDLARSNTRPSVGDVVRIVPNHVCVVVN
                     MMDEVVMVRGDEIIGTLPVAARGKLR"
     gene            complement(1970..3778)
                     /locus_tag="VGA15_21245"
     CDS             complement(1970..3778)
                     /locus_tag="VGA15_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9590259.1"
                     /translation="MAPPLIQLKDIKLTFGGTPLLSGVELSVSQSERVCLIGRNGSGK
                     STLLKIAAALVEPDSGSRFVQPGATIRYLPQEPDFSGFATTLAYVEAGLGPGDDHYQA
                     RYVLEQLGLHGDEDPATISGGEARRTALARVLAPSPDILLLDEPTNHLDLTTIEWLES
                     ELGSRRSALVIISHDRRFLSNLSRSTAWLDRGQIRQIDRGFSAFEAWRDEVLAEEERD
                     QHKLDRKIVNEEHWLRYGVSGRRKRNVKRLGNLYALREQRRDYRGAAGNANLAASEAD
                     KSGKLIIEAKHIGRTYGDRQIVDDFSIRVQRGDRIGIVGPNGAGKTTLIDMLTGANPP
                     DTGTIRFGANIEMATLDQHRESLDPKSTLADALTGGGSDNVMVGGKPKHVVSYMKDFL
                     FAQEQMRTPLEVLSGGERGRLMLARALAKPSNLLVLDEPTNDLDLETLDVLEEMLGDY
                     EGTVILISHDRDFLDRVVTSVIAPEGDGRWIEYAGGYTDMLKQRGADLKREAVKAAAE
                     EKKELKAGAPSGTAKRRLNFNEKHALETLPKTIAKLQAEIAKQQRHLDDPNLYKKDRK
                     KFDQASDALTKAQKELQEAEDKWLELEVLREEIEQA"
     gene            complement(3790..4279)
                     /locus_tag="VGA15_21250"
                     /pseudo
     CDS             complement(3790..4279)
                     /locus_tag="VGA15_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609125.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
     gene            4446..5246
                     /locus_tag="VGA15_21255"
     CDS             4446..5246
                     /locus_tag="VGA15_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590260.1"
                     /translation="MTSRIEPYRVLAHNTSKQSENKIHDDAVARRFGFSGGLVPGVDV
                     MAYMIHLPVAKWGRAFLERGLIEARFVKPVYDGEMADVTGEESDGVLAIEVKSRGQLC
                     ASGTASLPSSAPPVSISDFSETAAVAGRRPVEPASYELGKWLGTLPRGWAGEAANEYL
                     TDVRERDPIYANEGLGHPGLLQRVMNMVLVDNAILGPWIHVGSRMQLLSAAKSGDELT
                     ARAKVIGNYEKKGHRFVELDALVVANGRTPLAHCQHIAIYQPREQAAA"
     gene            complement(5288..>6650)
                     /locus_tag="VGA15_21260"
     CDS             complement(5288..>6650)
                     /locus_tag="VGA15_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9590261.1"
                     /translation="LGAYITGVAPSPLSLNVGKNLASVYRTPLMSVDIKTVLTNTTLM
                     GAYRGAGRPEANYFMERLIDRAADEMGINRLTLRKRNFIKPAQMPFAASSGVTYDSGD
                     FQGVFNKAIEISDHAGFAKRKRESRKQGKLRGIAIGSYLEVTAPPGVELGKVVFEDDG
                     SVRLITGTLDYGQGHATPFAQVLCDQLGVPFESVKLEQGDSDIVHTGNGTGGSRSITA
                     SGMAIVEASKLVIEKGKRAAAHLLEAAEADIEFGNGRFTITGTDRSIDIIELARRMRE
                     GKMPEGVPSSLDVDHTTEPVPSTFPNGCHVAEVEIDPETGIVQIVRYTGVNDFGTIVN
                     PMLVAGQLHGGVAQGIGQALMECVSYDSSGQPITGSFMDYAMPRAEDIPMMAVGDYPV
                     PAKSNPLGTKGCGEAGCAGSLSTIVNAVIDALSEYGIAHIDMPLTPERVWCAIQDAKA
                     KVA"
BASE COUNT         1123 a         2015 c         2183 g         1329 t
ORIGIN      
        1 ggcgtcgtta tcgcccacag ccctcgcccg attgatcaac gccgtaccga ttgagctcaa
       61 tggcgtggcg ccgatcgcgc gcgcgatttc gaccgcgggc ggaattgctt tcagcgaact
      121 cgatgccgac ttcatgatcc atcgcttgcc gggcattttt gccgccggcg aaatgctgga
      181 ttgggaagcc ccgaccggcg gctacctgct gcaggcctcg ttcgcgacgg gagcggcggc
      241 ggggaggggt gtgttgaagt ggctcaatcg tagaataggt tgagtgtaac gaaacccatc
      301 atgccccggg aagtttgaag gatttcacgg agttcatcat cgggcgcgca ttcgcgcgac
      361 ccgttggttc aacctatcct acaagacgca cgttaccgcg cggacgatga tgttcagatt
      421 ccgttcagcg gcggtcgctt aggtactgcg tccggagggc tgcaacaaag aggagacaga
      481 atgatcttga aaccattgat ggcggccgtg gcgatggcgg ccgctgtatc ggcaatgtct
      541 gttgtcgtgc cgtcatctgc ttacgctcaa agcggcgctg aggcggagat cattggattc
      601 catgacctct gcaacaaagg cgaccgtcgg gcctgcgtgc gctttggtat cctgattggt
      661 cagaaccagc agcgtcacgc cgactggcgc agggcccacg ccgactggtg gtggtgggaa
      721 aaacaataac gaaaggcatg aggccgctgt agccggcggc ctcatctcgc cgggtcaccg
      781 cagcttccct cttgccgcga ccggcagcgt gccgatgatt tcgtcgccgc gcaccatcac
      841 cacctcgtcc atcatgttga cgacgacgca gacatggttc ggcacgatcc gcacgacgtc
      901 gccgacactg ggacgggtgt tgctgcgcgc aagatcgaga aagccgtgtt cctcggcaaa
      961 gcgcgcgatt ttggcctcgg ggtgttcgag gatcaggcca tggccgtcga gcccgccgcc
     1021 ggtatcggag gtaagggttt tcgagccggc atccagaatg ccgcggtcgg ggccggcgcg
     1081 gctcaccacg gtcgaataga tgttcaatgc gcaatcgtcc caggtcgcga cgccggccgc
     1141 gacctgcatc cggtcgttgt agatgtaggt gccgggccgg tgctcggttg cgcccttgag
     1201 ttttccgacg ttttttagat ttggcgttcc gccagttgag acgatggccg catcaagtcc
     1261 gtgttcgcga atgccggcca gcgcctcgtc gtaaaaacgt tgcgcatctc cccagccggt
     1321 ctcggtcgga tacagcatga aaccggcaaa ggtcagtccc ttcgaagcgg cgatctggcg
     1381 tgcgagcgcg atggcttccg ctggagtctc gacgccggcg cgcttgcggc cggtgtcgca
     1441 ttcgaccacg acggagagcg gccggccgga agccgccgcc gcctgcggta accctgcgat
     1501 gacaaccgaa ttgtcggcgg cgacggtcat gttggtcctg gcctgcagcg cgccgagccg
     1561 cgccatcttc tcgtcgccga tcaagttgta gctgatcagg atgtcgtcga tgccggcctc
     1621 cgccatcact tcggcctcgc cgagtttctg gcaggtgatg cccctggcgc cggcggcgat
     1681 ctgaagtttt gccagcagcg ggcttttgtg ggtcttgatg tgcggccgat tggcgacgcc
     1741 ggcggcatcg caggcggctt gcacccgcgc gatgttgcgc tcgacgcgat ccatgtcgat
     1801 gacagctgcg ggcgtgccgt attcgcgggc gattttggcg gcaaggggtg tggtcatggc
     1861 ctgttctatc tcgttgttca aaccgtagga tgggtagagc gcagcgaaac ccatcaaacc
     1921 tcgtcggctt cttatgatgg gtatcgctgc gctcaaccca tcctacggcc taagcctgtt
     1981 cgatctcttc gcgtaacact tcaagttcca gccatttgtc ttcggcctcc tgcaattcct
     2041 tttgcgcttt cgtaagtgca tcagaggctt ggtcgaattt cttgcggtcc ttcttgtaga
     2101 gattgggatc gtcaaggtgc cgctgctgtt tggcgatttc ggcctgcaat ttggcgatcg
     2161 ttttgggcag ggtttccaac gcgtgctttt cgttgaaatt gagccggcgt tttgcggtgc
     2221 ccgaaggcgc gccagccttc aattcctttt tctcctcggc ggcggccttc accgcctcgc
     2281 gcttcaaatc cgcgccacgc tgcttcagca tgtcggtata accgccggca tattcgatcc
     2341 agcggccgtc gccttccggc gcgatcaccg aggtcaccac gcgatcgagg aaatcgcggt
     2401 catggctgat caggatcacg gtgccctcgt agtcgccgag catttcctcc agcacatcga
     2461 gggtttcgag atcgaggtcg ttggtcggct cgtccagcac caggaggttg gacggttttg
     2521 ccagcgcgcg cgccagcatc agccggccgc gctcgccgcc ggagagcact tcgagcggcg
     2581 tgcgcatctg ctcctgcgcg aacagaaaat ccttcatata gctgaccacg tgcttcggct
     2641 tgccgccgac catcacgttg tcgctgccgc ccccggtgag cgcatcggcc agcgtcgatt
     2701 tcggatcgag gctctcgcgg tgctggtcga gcgtcgccat ttcgatattg gcgccgaacc
     2761 ggatggtgcc ggtatccggc ggattggcgc cggtcagcat gtcgatcagc gtggtcttgc
     2821 cggcgccgtt gggcccgacg atgccgatgc ggtcgccacg ctgcacgcga atcgagaagt
     2881 cgtcgacgat ttggcggtcg ccataggtcc tgccgatgtg cttggcttcg atgatcagct
     2941 tgccggattt gtccgcttct gatgcggcga ggttggcatt ccccgcggcg ccacggtaat
     3001 cgcggcgctg ctcgcgcagc gcgtagagat tgccgagccg cttgacgttg cgcttgcggc
     3061 ggccggacac gccgtagcgc agccagtgct cttcgttgac gattttgcga tcgagcttgt
     3121 gctggtcgcg ctcttcctcg gccagcacct cgtcgcgcca ggcctcgaac gcactaaagc
     3181 cgcggtcgat ctgccggatc tggccgcggt cgagccacgc ggtagagcgc gacagattgg
     3241 acaggaagcg gcggtcatgg ctgatgatga ccagcgcgct gcggcggctg ccgagttcgc
     3301 tttccagcca ttcgatggtg gtgagatcga gatggttggt gggctcgtcg agcagcagga
     3361 tatcgggcga gggcgccagc acccgcgcca gcgccgtccg gcgcgcttcg ccgccggaga
     3421 tagtcgcggg gtcttcatcg ccgtggagcc caagctgctc cagaacgtag cgggcctgat
     3481 aatgatcgtc gccggggccg aggccggctt cgacataggc cagcgtcgtt gcgaatcccg
     3541 aaaagtccgg ctcctgcggc agatagcgga tggtggcgcc gggctgcacg aagcggctgc
     3601 cgctgtcggg ttcgaccagg gcggcggcga tcttcagcag cgtcgatttg cccgaaccgt
     3661 tgcggccgat caggcacacg cgttcggatt gcgagaccga cagttcgacg cccgacaaga
     3721 gcggcgtgcc gccaaaggtc agcttgatgt ctttcaactg gatcagcgga ggcgccatcg
     3781 tttttcaatt caatcttgtt cttgctcggg ctggtcggcc cgctggcgct ggatgcggcg
     3841 gatggtctga tcgagcgcgg acagaaacgc cgagcggtcg cgcggcgcga acgatcgcgg
     3901 gccgccggtc acttcgccgg atgaacgtaa atcggtcatc aggttgcgaa ccgcaagggt
     3961 catcccgatc gattgctcgg taaaaggctt gccgttcggc gcgatcacct cggcgcctgc
     4021 cttgacgcag cgactcgcca gcgggatgtc ggaggtaaca acgatatcgc cttttccggc
     4081 gcgctccgca atccagtcgt cggcggcgtc catcccggaa cccgccgcga tgcgctcgat
     4141 caagcggcac ccggatgaat tggcctgcga ccacgctgac gggcaggcca tggcgggccg
     4201 cgacacggta gatctcgtct ttgaccgggc aggcgtcggc gtcgacatag atgcgggtgg
     4261 tgttcaagaa gggcgtcatc ggttgggatt taggcgggga acgcaacggg tattcccgga
     4321 tgcacgcgtg gtaccccatc gggggcggaa ttgcgaggaa aacagcggat ggaacgcgct
     4381 tgccacggta ttttgttcgc gtacgattat cggcagaaaa gcgcgttaaa tcagaggaaa
     4441 caggcatgac cagccgcatc gagccgtacc gcgtcttggc ccacaacacc tcaaagcaat
     4501 ccgaaaacaa gatccatgac gacgccgtgg cgcgccgttt cggcttcagc ggcgggctcg
     4561 tccccggtgt cgatgtgatg gcctacatga tccacctgcc ggtcgcgaaa tggggccgcg
     4621 cgttcctcga gcgcgggttg atcgaggccc gcttcgtcaa gccggtctat gacggcgaga
     4681 tggctgatgt caccggcgaa gagagcgatg gtgtgctggc gatcgaggtc aagagccggg
     4741 ggcaactatg cgccagcggc actgcatcgc tgccttcatc cgcgcctccg gtctcgattt
     4801 cggatttcag cgaaaccgcc gcggtggccg ggcgcagacc ggtcgaaccg gcgtcctatg
     4861 aactcggcaa gtggctcggc accttgccgc gcggctgggc cggcgaagcg gcaaacgaat
     4921 atctgaccga tgtgcgcgag cgtgatccga tttacgccaa tgaaggcctt ggccatcccg
     4981 gattgctgca gcgggtgatg aacatggtgc tggtcgacaa cgccatcctg ggcccatgga
     5041 ttcacgtcgg cagccggatg cagctcttgt cggcggcgaa aagcggtgac gaactgacgg
     5101 cgcgcgcaaa agtcatcggc aattacgaaa agaagggcca ccgcttcgtc gagctcgatg
     5161 cgctggtcgt cgccaacggc cgcaccccgc tggcgcattg ccagcacatc gcgatctacc
     5221 agccaagaga gcaggcggcg gcgtagcctc ttaacctcgc cccgcttgcg gggcgaggtg
     5281 aagaagctca cgccactttc gccttggcat cctggatcgc gcaccagacc cgctccgggg
     5341 tcagcggcat gtcgatatgc gcgatgccgt attcggacag cgcatcgatc accgcgttga
     5401 cgatggtgga gaggctgccg gcgcagccgg cttcgccgca gcccttggtg cccagcggat
     5461 tggacttggc gggcaccgga taatcgccga ccgccatcat cgggatgtct tccgcgcgcg
     5521 gcatcgcata atccatgaac gagccggtga tcggctggcc gcttgaatcg tagctgacgc
     5581 actccatcag cgcctggccg atgccctgcg cgacaccgcc atgcaattgt ccggcgacca
     5641 gcatcggatt gacgatggtg ccgaaatcat tgacgccggt gtagcgcacg atctgcacga
     5701 tgccggtttc ggggtcgatc tcgacttccg cgacgtggca gccattggga aacgtcgacg
     5761 gcacgggctc ggtggtatgg tcgacgtcga gcgacgacgg cacgccctcc ggcatcttgc
     5821 cctcgcgcat ccggcgcgcg agctcgatga tgtcgatgct gcgatcggtg ccggtgatgg
     5881 tgaaacggcc attgccgaat tcgatgtcgg cttcggcggc ttccagcaga tgcgcggccg
     5941 cgcgtttgcc cttctcgatg accagcttcg aggcttcgac gattgccatg ccgctcgcgg
     6001 tgatcgagcg cgagccgccg gtgccgttgc cggtatggac gatgtcgctg tcaccctgtt
     6061 ccagcttgac gctttcgaag ggtacgccga gctggtcaca gagcacttgc gcgaatgggg
     6121 tcgcatggcc ctggccatag tcgagcgtgc cggtgatcag ccgcaccgaa ccgtcatctt
     6181 cgaacacgac cttgccgagt tcgacgccag gaggcgcggt gacttcgaga taggagccga
     6241 tcgcgatgcc tcggagtttt ccctgtttgc ggctctctct tttgcgtttg gcgaaaccgg
     6301 cgtgatcgga gatctcgatc gccttgttga agacgccctg gaaatcgccg ctgtcatagg
     6361 tgacgccgga tgacgcggcg aacggcattt gcgcgggctt gatgaaattg cgcttgcgca
     6421 gcgtcagccg gttgatgccc atctcgtcgg cggcgcgatc gatcagccgc tccatgaaat
     6481 agttggcctc ggggcgtccg gcgccgcggt aggcgcccat cagcgtggtg ttggtcagca
     6541 cggtcttgat gtcgacgctc atcagcggcg tgcggtagac gctggcgagg ttcttgccga
     6601 cgttcaggga taacggactt ggcgcgacgc cggtaatata ggcgccgaga
//
LOCUS       DASWGM010000771         7406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1311206,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000771 DASWGM010000000
VERSION     DASWGM010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311206"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VGA15_21265"
     CDS             <1..550
                     /locus_tag="VGA15_21265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9590262.1"
                     /translation="MFITMPDHAGTHIDAPRHFDKTGQPISEYPLENCIVPGICIDLR
                     HIAPRAEITPSDLEAAVEKAGVPVPKGGTVLLCTGHHERTFPRKEYATDNSGVNVAAT
                     EWLARQGIVHFGIDSMRPGPEGKVNLLVHKACFDLDITHIESLCNLEALLGAGQFTFI
                     GLPMKWRDGTGSPIRAVAVFDM"
     gene            618..1628
                     /locus_tag="VGA15_21270"
     CDS             618..1628
                     /locus_tag="VGA15_21270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9590263.1"
                     /translation="MSGKKSAHRSRREVLRAAGLFTGAAVASCLGPAGATAAGYPERP
                     VKIIVPFAPAGPTDIMARILVAHLADAIGGTLIVENKPGAGGNIGIGAAAHAEPDGHT
                     LLITSSAYVVNPGLYAKIPYDPYTDFAPIAELGTSPNVILVDPKLGVNSIPDLIARAK
                     ANPDELNYASPGIGTTPHLSGELFKIVGRIQITHVPFSGAGPAIQAVLSGTTQIALAA
                     LPPARPHIESGALKALAVTGAHRWFDLPDVPTMVELGYTDFISDTFQGFLAPAKTPPA
                     IVDLLSTKSIEILKTPAIAEQLRNNGFEVIANGPDGMRKRIADEVPKWRDIVAKAGIK
                     PV"
     gene            complement(1662..1904)
                     /locus_tag="VGA15_21275"
     CDS             complement(1662..1904)
                     /locus_tag="VGA15_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9590264.1"
                     /translation="MLRPSPDLPISDASRRRLTRWLVGLPVRDVERDLVIETLASTRG
                     NRTASARVLGMSVRTLRNKISEYSAHGIDVPRPDRS"
     gene            complement(2124..2585)
                     /locus_tag="VGA15_21280"
     CDS             complement(2124..2585)
                     /locus_tag="VGA15_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590265.1"
                     /translation="MAATAVAERLTVAAAVLAPEPEVDNSDRADHQETVSPGDADMTA
                     SVSAIDPDFLVALVMARPEIRSVSDLANKNIAIDGEPSASNGNVRTGIVAAGAAEVQL
                     SKGEAKAIDRLIGGEVPAAVLTLVSAEAAEAFPDIAGFRIFRIPLSPKARL"
     gene            complement(3478..4446)
                     /locus_tag="VGA15_21285"
     CDS             complement(3478..4446)
                     /locus_tag="VGA15_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590266.1"
                     /translation="MKDFAGKTAVITGGGTGMGRELARQLVAEGCNVAMCDVSASAMA
                     ETKRLCEVERLPQGLRITTHIADVSMEDQLLRFRDELVEQQATDRIHLLFNNAGIGGG
                     GSLFTNTRERWEKTFNICWGGVYLGVRTFLPLLQKADEGHIVNTSSVNGFWASVGPGV
                     SHTAYSAAKFAVKGFTEALINDLRLNAPHIKCSVVMPGHIGTSIVSNSRKVQSGTDSE
                     RLNENEIAQTRVRLTTAGIDASMMSDEDVQAAAAERARSFREDAPTTAAAAAKIILDG
                     VKAERWRILVGEDAHRLDERVRQTPERAYEPEFYQSFAAEVGWRLG"
     gene            complement(4812..5042)
                     /locus_tag="VGA15_21290"
     CDS             complement(4812..5042)
                     /locus_tag="VGA15_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590267.1"
                     /translation="MERLGNLVKEHLILGTDKRDVEIQRDLWLSQNPALKVIKIHGVK
                     REPQTLLTRIGSKHVPRVSIVVEFEEPGVAAE"
     gene            complement(5228..5383)
                     /locus_tag="VGA15_21295"
     CDS             complement(5228..5383)
                     /locus_tag="VGA15_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590268.1"
                     /translation="MAALAKVLLRVFPKSRIDVEILKTIAMFCGVGLLVSLLLATRGL
                     DMSAGFF"
     gene            5907..6056
                     /locus_tag="VGA15_21300"
     CDS             5907..6056
                     /locus_tag="VGA15_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590269.1"
                     /translation="MEKFIHRENPALFKKRLADVRDESERQVLLKLLAQEDAKDSPRP
                     PRLKS"
     gene            6053..6274
                     /locus_tag="VGA15_21305"
     CDS             6053..6274
                     /locus_tag="VGA15_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590270.1"
                     /translation="MNRRGIEFTLAQVEPGLWRWRFQIGDKVTTGNTKANLMGLATRR
                     VQQRIDIELKKAVRPGFWQPNSASPPDAP"
     gene            complement(6886..>7406)
                     /locus_tag="VGA15_21310"
     CDS             complement(6886..>7406)
                     /locus_tag="VGA15_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX9590271.1"
                     /translation="PIMDFSAASGLRLNECLLKWTEVNWGTRQIQKQGKGKRRVTVPI
                     TPVIREILWPLRGHHSEFVFTYVAVRTRGERVRGKRQPITYNGLKTTWRRLRSAAKVA
                     DFRFHDKRHDLATKLLRKTGNLKLVQKALNHRNLKTTLKYAHVLDEEVAAGLQQVQKS
                     RRKSRKHVRVAD"
BASE COUNT         1469 a         2272 c         2173 g         1492 t
ORIGIN      
        1 gatgttcatc acgatgcccg accacgccgg cacccacatc gacgcgcccc gacacttcga
       61 caagaccggc cagccgatca gtgagtaccc gctggagaac tgcatcgtgc cggggatctg
      121 catcgatctg cgccacatcg cgccgcgtgc cgagatcacg ccctccgatc tcgaagcggc
      181 cgttgagaaa gccggcgtgc cggtgccgaa gggcggcacc gtgctgctct gcaccggcca
      241 tcacgagcgc acctttccgc gcaaggaata tgcgaccgac aactccggcg tgaatgtcgc
      301 cgccaccgaa tggctggcca ggcagggcat cgtgcatttc ggcatcgact cgatgcggcc
      361 cggcccggaa ggcaaggtga atttgctcgt tcacaaggcg tgtttcgacc tcgatatcac
      421 ccacatcgag agcctgtgca atctggaagc actgctcggc gccggccagt tcacctttat
      481 cggcctgccg atgaagtggc gcgacggcac cggctcgccg atccgcgcgg tggccgtgtt
      541 cgatatgtga gacgcagacc aagctgaaac aaggtgaccc ggcgcaagcc gggcaattca
      601 ggccggggag aaacacaatg tccggtaaaa aatccgcgca ccgatcgcgc cgcgaagttt
      661 tgagggccgc cggcctcttt accggagccg ccgtggcgtc atgcctcggg ccggccggtg
      721 cgacagcggc gggctatccc gaacgccctg tcaagatcat cgtgccgttc gcgccggccg
      781 ggccgaccga tatcatggcg cgtattcttg tcgcgcatct cgccgacgcc atcggcggca
      841 cgctgatcgt cgaaaacaag cccggcgccg gcggcaatat cgggatcggc gctgcggcac
      901 acgccgagcc ggacggccac acgctgctga tcacgtcgag cgcctacgtc gtcaatcccg
      961 gtctctacgc caaaatcccc tacgatccct acacggattt cgcgccgatc gccgaactcg
     1021 gaacttcgcc aaacgtaatt ctggtcgatc cgaagctcgg cgtgaattcg attcccgacc
     1081 tgatcgcgcg tgccaaggcc aatcccgacg agctcaatta tgcgagcccc ggtatcggca
     1141 cgacgccgca tctgtccggc gaactgttca agatcgtcgg ccggatccag atcacgcatg
     1201 tgccattctc cggcgcaggc ccggcgatcc aggccgtgct cagcggcacc acgcaaattg
     1261 ccctcgcggc cctgccgccg gcacgtccgc atatcgaatc cggcgccttg aaggcgcttg
     1321 ccgtcaccgg cgcccatcgc tggttcgatt tgcctgatgt gccgacgatg gtcgaactcg
     1381 gctacaccga ttttatctcc gacacgttcc agggctttct ggcacctgcg aaaacgccgc
     1441 ctgccatcgt cgatctgcta tcgaccaagt cgatcgaaat tttgaagacg cctgctatcg
     1501 cggagcaatt gcgcaacaat ggcttcgagg tcatcgccaa cgggcccgac ggcatgcgca
     1561 agcgcatcgc cgacgaggtg ccgaaatggc gtgacatcgt cgccaaggcc ggcatcaagc
     1621 ccgtctgatc aacattcagc gggctcgatg cggggtcttc ttcagcttcg atccggccgc
     1681 ggaacgtcga tgccatgggc cgaatattca ctgatcttgt tccgcaacgt gcgaacggac
     1741 atgccgagca cacgcgccga cgcggtacga tttccacggg tgctggcaag tgtctcgatc
     1801 acgagatcgc gttcgacatc tctgacaggt aagccgacca gccatcgcgt cagacggcgt
     1861 cgcgaggcat cagatatcgg caaatcgggg gacggccgca gcatgcgaga tactccactg
     1921 tacaaacgat tctcaacccc gccccagagg acaagaacaa ggttaacgaa ttcttaacgg
     1981 gatctttaaa tttggaatcg gacaaaccgg cgggcgccgt atgtcggcca cattcctgaa
     2041 gaggcagagc cggccgattc gccgattcag cgtcgaccag attcgagcag gcgatcaacc
     2101 ggggttcttc aacaccacac caactagagt cgcgccttgg gcgacagcgg gattcgaaaa
     2161 atcctgaagc cggcgatatc gggaaatgct tccgccgctt ccgccgatac caacgtcaaa
     2221 accgcagccg gcacctcgcc accgatcaac cgatcgatcg ctttcgcctc acctttgctt
     2281 agttggactt cagccgcccc cgccgccacg atcccggtcc tgacattgcc gttggacgct
     2341 gatggctcgc catctatcgc aatgttttta ttggccagat cggataccga tctgatttcc
     2401 gggcgcgcca tgacgagggc aaccagaaaa tcaggatcga tcgccgagac ggatgcggtc
     2461 atgtcggcat cgccgggcga cacggtttcc tgatgatcgg cacggtcgct attatcaact
     2521 tccggttccg gcgccaaaac cgcggcggcg accgtcaatc gctccgcgac cgccgtagcg
     2581 gccatcacca ggtcccgtgg tgttcgcgtc gtggaagcgg cagccgcatc agccgtggga
     2641 tgcgaatccg cgatccttgt ttgcgcaggt tcggaagtgt tggcggcgct ggccgcgggc
     2701 tgcggatcca cgttctttgc ttgcgtgggt ttggaatcgg cagcgagacc cgccagcctc
     2761 gcgtgcggca tttcgttgga atttggtgcg gcctgtctgg aatttgctgc gccctgtctc
     2821 acgacacgag catgcgccgc gggcttcgct ctttgtccgc ggatcgctat ctcgcggtgg
     2881 accaaagctt tcggcagaac ctgcggaggt tgcccagctg cgcgctgcgc ctgtatcgac
     2941 aacgattgcc gggaccacgc catataggac tggcaaaggc tgttacatgg caatccgtcg
     3001 acatacacga tgtcaggtgg atccagcggc gtggcttccg aatttcccgc agaaccgaga
     3061 aacaaaacgc aggacagcaa tacccatttc ctcatcatga ctactccatt tgggcgcgca
     3121 gatgtttggg gcgccgaata ttcgatggag gctcacgcac gacggaacaa tcacggtccg
     3181 tgcggcccta aaaaatctaa taaaggccgg ttagctcacg attccgacga aaaaacggcc
     3241 gggtcttgca tcggtatttc tgaaccgcgt tttgccggaa agatgcattc tcgatcgtca
     3301 gaaggcctgc gtaacgagca ctcaaccacg cgagctccga tcacaactgc agcacggatg
     3361 atggtactac cgtgagagat tttcttcgcc gcgtctctcg cgtgcgcaac ttcaggtgtc
     3421 gcgcacctac tcgaccacta tggcgcgccc tgaaaaatcg ctcgcactgg atcgaggtca
     3481 gcccagccgc cagccgacct cggcggcaaa gctctggtag aactcaggct cgtaagcgcg
     3541 ctccggtgtt tgccggacgc gctcatcgag gcggtgggcg tcctcgccaa ccagaatgcg
     3601 ccagcgctcg gccttgacgc catcgagaat gatcttcgcg gcagcagccg cagtggtcgg
     3661 cgcatcctcg cgaaaactgc gggcgcgctc ggccgcggcg gcctgcacat cctcatccga
     3721 catcattgat gcgtcgatgc cggccgtggt gaggcggacg cgggtctgcg cgatttcgtt
     3781 ctcgttcagg cgctcggaat cggtgccgct ctgtactttt cgcgaattcg aaacgatcga
     3841 ggttccgata tggccgggca tgaccacgga gcacttgatg tgcggcgcgt tgagccggag
     3901 atcgttgatc agcgcttcgg taaatccctt caccgcgaat ttggcggcac tataggcggt
     3961 gtgcgatacg ccaggtccga cggaagccca gaacccgttg acgctggagg tattgacgat
     4021 gtggccctcg tcggcctttt gcagcagcgg cagaaaggtg cggacgccga gatagacgcc
     4081 gccccagcag atgttgaagg tcttttccca ccgctcacgc gtattggtga acaggctgcc
     4141 gccgccgccg ataccggcgt tgttgaacaa cagatggatc ctgtcggtgg cctgctgctc
     4201 gacgagttca tcgcggaagc gcagcaactg gtcctccatg gaaacgtcgg cgatgtgggt
     4261 ggtgatgcgc aggccctgcg gcagcctttc cacctcgcac agccgcttgg tttccgccat
     4321 cgcgctcgcc gacacatcgc acatcgcgac attgcagccc tcggcgacca gctggcgcgc
     4381 gagttcgcgg cccatgcccg tgccgccccc ggtgatgaca gcggtctttc cggcaaaatc
     4441 tttcattggg gggatcgctg acctttccgg ttctgatttg cgtgatttgg tcggagcgag
     4501 aggattttga acctccaccc aagcctcccg gacgttgaaa ctggcgtctc acgtttgttt
     4561 cagactagtg cacaagccac agtgcaactc aaatactttt ttcttcctgt gttttcgcgg
     4621 gagtgcggcg ggtttcgcaa ttcaatcatg aaggccgtga ggccgcagcg ataacccatt
     4681 ggaggttgag aagccgccgc tagcgcattg aacccgcggc gcttgtttca ggcacgctcg
     4741 acggggtttt gctgaccgca gcgatcgcga tatttctcct gatcgcgttc ttccgatgga
     4801 aacgccgggc tttattccgc ggcgacgccg ggctcctcga actccaccac gatcgaaacg
     4861 cgcggcacat gcttgctgcc gatacgcgtg agcaaggtct gcggctcacg cttcactcca
     4921 tgaatcttga tgaccttcaa ggcgggattt tgcgagagcc acaggtctct ctgtatttca
     4981 acatcccgct tgtcggtgcc cagaataaga tgctctttca cgagattacc cagacgttcc
     5041 atcgactcgc actctcaaaa tgacgccggt gtttcattac gcgtgctcta gatgagctga
     5101 tttggcacat gtggaagggt cttcgcatgt tccggaagct aatcgcaaga accatgccaa
     5161 gccggcgcaa gccttgggag gctcgcgcgt cgcgcattcc gcgcgggatg aagggtgacg
     5221 gccgccgtca gaaaaatccc gcactcatgt ccaggccgcg cgtggccagc agcaaagata
     5281 ccaacaggcc aaccccgcaa aacatggcga tggtcttgag aatttcgacg tcaatccgac
     5341 tcttgggaaa cacgcgcaag agaacttttg ccagagcagc cattttgcga cctccaagct
     5401 cgaccccagg gatgaggcca gttggagcgt tggtagctca gccggcgcgc cgcgcatgtg
     5461 agtaaggtca cactaacgaa ttaccccggc gggaatggtt ccagtgccag cgggccagtc
     5521 gcacattctt tttattcgga gagtgagcaa tattgatcac ggagtaagaa gccggttgaa
     5581 gcaggatcta gcgatcaccg ctccgccctt ggcatttatc ggtgacagag agtttggtaa
     5641 cgctcccggg cgtgtgtcat gacccaactg accctcgttc cattcagatg ccgatttgaa
     5701 acggcgtcgg ctgacgtaac ggccagcgga tcgcaaggcg agttttgcgc ttttgagttt
     5761 cctggctgcg aggggtgccg atacgatctg cacgccatca aaacggcagc cttcggagac
     5821 ccggtgaagt cgaccgcttg gcgcggatgg atcgcaggcg cgtcccgccc cacaaaatag
     5881 ttttcgcacc atgagggagg gccgctatgg agaagttcat tcaccgggaa aatccggcgc
     5941 tcttcaaaaa acgcctcgcg gacgttcgcg atgagtccga gcgccaggtg ctcttgaagt
     6001 tattggcgca ggaagacgct aaagactcgc ctcggccgcc gaggttaaaa tcatgaaccg
     6061 caggggtatt gaattcaccc tggcgcaggt tgagcccggt ctgtggcgat ggcgattcca
     6121 gatcggcgac aaggttacga ccggcaacac caaagctaac ctcatgggct tggctacccg
     6181 cagagttcag cagagaattg atatcgagct aaaaaaagcc gtccgaccgg gcttctggca
     6241 gcccaacagc gcgagcccgc ccgatgctcc ctaaagccac gccgtccccg aaaaagagcg
     6301 cattgtccgt ttgacgtgcg tccgatacgc gattgcgcgg cgatcgccta acggatgccc
     6361 acaaaggctt gcgctcttgg tacctgcacg aggtgaggcg tttacactcc tcggggcgcg
     6421 ccggtcactt cgcggcaagc gagcggcgcg aggtcatcaa aatgatggcg gcaatgcgga
     6481 aagtctgcca caatgttaaa tggggcggcg caatatgatg gccttgcttc tagctctcgg
     6541 cctgctggct gttgttcttt acatgcccgc catggtcgca atcgactacc acgggcggcc
     6601 gcgcgcttga ggggtactgt ggacacggat atctacgcgt tcattgtcac ggtggcagcg
     6661 tggctggtgg tctttattct ggggtatttt cagcggcggg ctaccgcttc cggtcccagc
     6721 cgcggttggc gtgcttggct ctcacacgct gctgccgccg taaagttccc gatcttatat
     6781 ttggtttcgc ggggtaagtt ggtcggagcg agaggatttg aacctccgac ccctagtctc
     6841 ccagaaaagg acgccgactc tgatattcat cattatatca atgtgttaat cggccacgcg
     6901 aacatgtttt cgggattttc ttcgggactt ctgaacctgc tggagcccgg cggcgacctc
     6961 ctcgtcgaga acgtgggcat atttcagcgt cgtcttgaga tttcggtgat ttagggcctt
     7021 ctgaaccagt ttgaggttgc cagtcttgcg aagcagcttc gtcgcaaggt catgccgctt
     7081 gtcgtggaac cggaagtcag caaccttggc agcggaccgc agccgcctcc acgtcgtctt
     7141 aagtccgttg taggtgatcg gctggcgctt gcccctcacc cgctctccgc gcgtcctgac
     7201 ggcgacatag gtgaatacga attcggaatg gtgtccccgc agtggccaga ggatttcccg
     7261 aatgaccggc gtgatgggga ccgtcacgcg cctcttgccc ttcccctgct tctggatttg
     7321 ccgcgtaccc caattgacct cggtccattt gagcagacac tcgttcagac ggagaccgga
     7381 cgcggcagaa aagtccatga tcggct
//
LOCUS       DASWGM010000772         5753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2037660,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000772 DASWGM010000000
VERSION     DASWGM010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2037660"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VGA15_21315"
     CDS             complement(<1..408)
                     /locus_tag="VGA15_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX9590272.1"
                     /translation="MGKRSVLAAILAGVVGILIGPAFSQEVKKEAKSTAGAAKIAPVT
                     QSELNTAAKSTDNFLLTNGNYAQTRFHPARQILRENVKNLHVAWIFQTDVKESLETSP
                     IVVDGVMYVTTSFSHVYALDAKTGAQLWHYNHKM"
     gene            974..1744
                     /locus_tag="VGA15_21320"
     CDS             974..1744
                     /locus_tag="VGA15_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX9590273.1"
                     /translation="MATSNTNLDCFGDRGPLLRWLVFTGLCVFAFVLLWRFGLIRLMV
                     ASDRTYLSSIIGLLYIAASMHCLWRTIMISREGDAARRGGAIIAAGSGGLDVDGDAVV
                     VEGAGKLPPGLVSAHIHDLAVKARLQGPRRLDQTLLLRGLAGRLRGSNQFGAFASDTL
                     MKLGLLGTIIGFIMMLAPIAGLDPNDRGAIRSSMNLMSDGMAVAMYTTLAGLVGSILV
                     RIQYYMLDDATAKLFAYAVSLTEVRVVSILEDQPGAAE"
     gene            1741..2373
                     /locus_tag="VGA15_21325"
     CDS             1741..2373
                     /locus_tag="VGA15_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590274.1"
                     /translation="MIDEFDLMPQEEPFDPFAVMLFKALQVIAFLFFIALLAIAPEAK
                     DGKVDSKAEFLVTMTWPDQHPDDFDMFVQDPLGNMVWYRRREAGFMLLDRDDRGGAND
                     FILVNGRKILSPIRQETVSIRGIVAGEYTVNVYHFVAMGREPVPVSVKVEKLNPTVQV
                     VHYATIMLEAAGTERTAVRFSIDAKGGIVDVNNHEKSLLQTLRNPRRNGG"
     gene            2379..2990
                     /locus_tag="VGA15_21330"
     CDS             2379..2990
                     /locus_tag="VGA15_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590275.1"
                     /translation="MELQKEILGLSIAYALLAALLLVIALRARMPWPLKIACVLVTSA
                     YYCVAFFHVEGLPGWSATAPLPPQFQLLWARVVEPNPLDRDPGAVHIWVEDLDAANLP
                     SGQPRAYRLPYSAALAAKVEAARDEIMKGHPQGGRAADFGAGNGQPAPEGPSDVSTFR
                     PSAAPGGDPTGGGPLDLSFLTGESGNIEFAPLPVPILPAKDTP"
     gene            complement(3127..3519)
                     /locus_tag="VGA15_21335"
     CDS             complement(3127..3519)
                     /locus_tag="VGA15_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590276.1"
                     /translation="MKRIILFLSIFGCLIASVNARDLGQWDAVDPAIRQWYQALMQPD
                     VPNASCCGEADAYWADEVHVRDGKTYARITDDRDDAPRGRPHIDIGTEIEIPNNKLKW
                     DKSNPTGHGIVFLSRAGYVFCYVQPGGV"
     gene            complement(3859..5178)
                     /locus_tag="VGA15_21340"
     CDS             complement(3859..5178)
                     /locus_tag="VGA15_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9590277.1"
                     /translation="MTLSQQSVWRGLDFPKSSGPEAVLQSAIRKAALRFVPLLTIAYL
                     FNYLDRTSLGFAALTMNQQLGLSAGQFGLAAGIFFLGYSLFEVPSNLLLYRYGARRWL
                     ARIMISWGLVSAATAFVVGPSSFYALRLLLGIAEAGFFPGVTFFLAAWFPAQYRTRML
                     AWFLIGIPASSLVGGPVCGMLLQMDGIWGLAGWQWLFILVSLPCLPLGLLTLYLLADR
                     PETAGWLTRDERDVLREVLSSEVRERPHSSLLAALKDTRVLILAAIQFGFTLGSYGIG
                     IWLPLILKEYHLSTWAIGWISAIPYLFASAGMILWARYVDLRGRRVFNLAITCFLGGA
                     GLVAPILSSSLAIALAGFSVALVGVTAARAIFWTIPTRFLTGVAAAGGLAFINSIGTV
                     GGFVGPSMMGWLREFSGSYVVGLTAVAVIMIAATVASMSLKLVVSKE"
     gene            complement(5397..5696)
                     /locus_tag="VGA15_21345"
     CDS             complement(5397..5696)
                     /locus_tag="VGA15_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX9590278.1"
                     /translation="MAVQMTKSQLIEKIAGETEVAKKQVKGVLETLATVGYKELKKSG
                     VFLVPGFAKFVVVKKPATKARKGVNPFNGEPMMFKAKPARKIVRARPVKAAKDAV"
BASE COUNT         1190 a         1742 c         1678 g         1143 t
ORIGIN      
        1 catcttgtga ttatagtgcc agagttgtgc gccggtttta gcgtcgagcg catagacatg
       61 gctgaacgac gtcgtcacat acatgacgcc gtcgacgacg atcggcgaag tctccagcga
      121 ttccttgaca tcggtctgga agatccatgc gacgtgcaga tttttcacgt tctcgcgcaa
      181 gatctgccgg gccgggtgaa agcgtgtctg ggcataattg ccgttggtca gcagaaagtt
      241 atcggtgctc ttggctgccg tgttgagctc gctctgagtg accggagcga tcttggccgc
      301 gccggccgtc gacttggctt ccttcttcac ttcctgcgaa aatgccggcc ctatcaggat
      361 accgacaact cccgccaaaa ttgcagctaa aacggaacgc ttacccatcc cttcctcccc
      421 aaatttttat cgttgatcgc gtagcgacat tcccgaatgc agagtcggac caacccaatc
      481 tggccggatg cgacaaattt gacaaggcga aaacgccaag cgcagagcac cgagcgccaa
      541 atgttttcgc gaacgacggg atcaaccgca tcggtcatct gcgaaagcaa aggccgcgtg
      601 aggtgtcagg ctcaaccttc aagctgattt cgaattcgat accgaccgat tcagcgacaa
      661 gaacatgtgg tcgcggtcgc gttgtctgct catcaaagcg tcttccagcg aagcatgccc
      721 ccggacttga tccgggggag gaggccggtt cgcggcaaga aaaacgcgcc aaatcaagaa
      781 tctagagccc cctttcgatt ctatcgaaac ggtaaaggct cgaggacagc agacctgtcg
      841 gggaaccgcg atgcagcata gccgcgataa accggcagat caggactgtc gggaaacttc
      901 ggttgcatgc atttgcgtgc tcgcgcggga tgtggaacgc ttggcttttc gggtcggatg
      961 tcgaggtgcc tggatggcaa cgtcgaatac gaatctcgat tgcttcggtg accggggccc
     1021 tctgttgcga tggctggtct tcaccgggct ctgtgttttt gcctttgtcc tgctgtggcg
     1081 cttcggactg atccgcctga tggtcgcaag cgaccgcacc tatctctcgt cgatcatcgg
     1141 tctgctctat atagcggcgt cgatgcactg cctgtggcgc accatcatga tttcgcgcga
     1201 gggtgatgca gcccgccgcg gcggagcgat catcgcagcc ggatcgggtg gccttgatgt
     1261 cgacggcgac gccgtcgtgg tcgagggcgc cggaaaactc ccgcccggtc tggtctcggc
     1321 gcacattcac gatcttgccg tcaaggcgcg cctgcagggc ccacggaggc tcgaccagac
     1381 gttgctgctg cgcgggcttg cgggccggct gcgcggctcc aaccagttcg gcgccttcgc
     1441 cagcgacacg ctgatgaagc tcggcctgct cggcaccatc atcggcttca tcatgatgct
     1501 ggcgccgatc gccgggcttg atcccaatga tcgcggcgcc atcagatcct cgatgaacct
     1561 gatgagcgat ggcatggcgg ttgccatgta caccaccctc gccggtctcg tcggctcgat
     1621 cctggtcagg atccaatact acatgctcga cgatgcaacc gcgaagctgt ttgcctatgc
     1681 cgtcagcctg accgaggtcc gcgtcgtttc gatcctcgaa gatcagcccg gagcggcgga
     1741 atgatcgacg agttcgacct catgccgcag gaagagccgt tcgatccgtt cgcggtcatg
     1801 ctgttcaagg cgctgcaggt tatcgcgttc ctgttcttca tcgcgctgct cgcgatcgcg
     1861 cccgaggcca aggacggcaa ggtcgacagc aaggccgaat tcctggtcac gatgacctgg
     1921 cccgaccagc atccggacga ttttgacatg ttcgtccagg acccgctcgg caacatggtg
     1981 tggtatcgcc ggcgcgaggc gggattcatg ctgctcgacc gtgacgatcg cggcggcgcc
     2041 aacgatttca ttctggtcaa cgggcgcaag atcctgtcgc cgatccggca ggagacggtc
     2101 agcatccgcg gcatcgtcgc cggcgagtac accgtcaacg tctatcattt tgtcgcgatg
     2161 ggccgcgaac cggtgccggt ttcggtgaag gtcgagaagc tcaacccgac cgttcaggtg
     2221 gttcactacg ccacgatcat gctggaggcc gcggggaccg agcgcacggc ggtccgcttc
     2281 tcgatcgacg ccaagggtgg gatcgtcgac gtcaacaatc acgagaaatc gcttctgcag
     2341 acgctgcgca atccgcgccg caacggcggc tgacgaacat ggaactgcag aaggaaattc
     2401 tcggtctgtc gattgcatat gcgctgctgg cggcgctgtt gcttgtcatc gcattgcgcg
     2461 cccgcatgcc gtggccgctc aagatcgcct gcgttctggt caccagcgcc tattattgcg
     2521 tggcgttctt tcacgtcgaa gggttacccg gatggtcggc gaccgcgccg ttgccgccgc
     2581 agttccaatt gctctgggcg cgggtcgtcg aaccaaaccc gctcgatcgc gaccccggcg
     2641 cagtacatat ctgggtcgag gatctcgatg ccgccaacct cccgagcggc caaccgcggg
     2701 cctatcgact tccttattca gcagcgcttg ccgccaaggt ggaagctgcc cgcgacgaaa
     2761 tcatgaaggg ccatccgcag ggaggacgcg cggcggattt cggggcgggc aatggccagc
     2821 ccgcgcccga aggtccgtcc gatgtctcga cgtttcgtcc ctcagccgcg ccgggcggcg
     2881 atccgaccgg cggcggtccg ctcgatctat cgttcctgac cggcgaatcc ggcaacatcg
     2941 aattcgcacc gctgccggtg ccgattctgc ctgccaagga tacgccctga acagtgctta
     3001 tagcgcacgc acacctattg ccgtccattt gctcggtgag cgctcttgat ctcgccgatt
     3061 caacaacaag caacttgcac gcgatttggg cattggatcc ggacgcagag cgcgattcga
     3121 atcgaatcag acgcccccgg gctgcacgta gcagaacacg taaccggcac gactcaagaa
     3181 tacgatgccg tgaccggtcg gattcgattt gtcccatttg agtttgttgt tcgggatttc
     3241 gatctccgtt ccgatgtcga tgtgcggcct gccgcgcggc gcgtcatccc ggtcatcggt
     3301 gatcctcgcg taggtcttgc cgtctctgac atggacttca tctgcccagt aggcgtcggc
     3361 ttcgccgcaa catgatgcat tcggaacgtc cggctgcatc agagcctggt accattggcg
     3421 gattgcggga tctaccgcgt cccattgtcc gagatcacga gcattcacac tggcaattag
     3481 gcaaccgaaa atactaagaa aaaggatgat acgcttcatc catctcgctc ctgtgttttc
     3541 cggcgagatg gtctaagcaa tgttcgtgcc gcgaatcaga tccccatgat acacttggca
     3601 aaattaaccg tgggcgcgat gagcgccggc ggctctattc gtacgattgc aggaggtggt
     3661 gaggaaatat ccgaccgatt cgagcctatc ggcttcttgt cgagagccgt gtcagcaata
     3721 gataccgata ggtacaagca tggcgcgtgg atgatttctc tgcgtcgcag ctcgcatcat
     3781 aaattctctg tgattgaatt ctaccgaacg cgaacggtgt taatcggagc gtggcgccat
     3841 tgggtagcct gcttgacatc actccttcga caccacgagt ttgagtgaca tcgatgcaac
     3901 ggttgcggcg atcatgatga cggccaccgc ggtcaggccg acgacgtaag atccggaaaa
     3961 ctccctcagc cagcccatca tcgaaggacc aacgaaaccc ccgaccgtgc cgatgctgtt
     4021 gatgaaagca agtccgcccg cggcggcaac gccggtcagg aagcgcgtcg gtatcgtcca
     4081 gaagatcgcc cgcgcggcgg tgacgccgac cagagccacg ctgaatcccg ccaaggctat
     4141 tgcgagcgag gaggataaga ttggcgccac aagacctgct ccgccgagaa agcacgtgat
     4201 cgccagattg aagacgcggc ggcctctgag gtcgacatag cgcgcccaca agatcatgcc
     4261 tgccgaggca aacaaataag gaatcgccga gatccaaccg atcgcccatg tcgacagatg
     4321 gtactccttc aaaatcaagg gcagccagat gccgatgccg taggacccca atgtgaagcc
     4381 gaactggatc gcagcaagga tcagcacgcg cgtatctttc agggcggcca gcaacgagga
     4441 atgcggccgc tctcgcactt cggaggagag gacctcgcgc aacacatcgc gctcgtctcg
     4501 cgtcagccag cccgcggtct ccggacgatc ggcaagcaag taaagcgtca ggagacccaa
     4561 agggaggcat ggcaagctga ccaggatgaa aagccattgc catccggcca gcccccagat
     4621 tccatccatc tgaagaagca tgccgcaaac cgggccgcca accagcgagg atgccggaat
     4681 cccgatcaga aaccacgcca gcatccgcgt gcggtattga gccgggaacc aggcggcgag
     4741 aaagaaggtt acgcctggaa agaacccggc ctccgcgatg cccagcagaa ggcgcagtgc
     4801 gtagaaactg ctgggaccaa cgacaaaggc ggtcgccgcc gacaccaggc cccagctgat
     4861 catgatgcgg gcgagccagc gccgcgcgcc gtaacggtag agcaacagat tgctcggcac
     4921 ctcaaacagc gaatagccca aaaagaaaat acccgcggcc aaaccgaact gaccggccga
     4981 cagccccagc tgttgattca tggtcaaggc ggcaaaaccg agactggtgc gatcgagata
     5041 attgaacagg taagcgatcg tcagcagcgg cacgaagcgc aaagcggctt tccgaatggc
     5101 gctttgcaac acggcttccg gaccggacga cttcgggaaa tcaagaccgc gccagacgct
     5161 ctgctgtgag agtgtcatct cctgcttcct tccccgccat gaatttcttg ttttgcttgt
     5221 tttgtaggtc gccagagcga agcggcgacg tcgccgccac gatttactgg cgagatttac
     5281 tcgcaagatt tgcctggcgt gccgacgccg tcaaagcata tctgcgtggc cggcaccgca
     5341 agatccgttg gcgaaggccg gaactgtaaa aggcccccga aggggccttt tgatattcac
     5401 accgcgtctt ttgcggcctt gacggggcgc gccctgacga tctttcgggc cggtttggcc
     5461 ttgaacatca tcggctctcc attgaatgga tttacgccct tccgcgcctt ggtcgcgggc
     5521 ttcttgacga cgacgaactt ggcaaagccg ggaaccagga acacgccgga tttcttcagc
     5581 tctttatagc cgaccgtcgc gagcgtctcc aaaacccctt ttacctgctt cttcgctact
     5641 tcagtctcgc cagcgatctt ctcgatcagc tgcgacttcg tcatctgcac tgccattttg
     5701 tctcctgtaa gttgattcgg cgtcacggac agtatttgga aatccagcca gaa
//
LOCUS       DASWGM010000773         9630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1042535,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000773 DASWGM010000000
VERSION     DASWGM010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042535"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VGA15_21350"
     CDS             <1..764
                     /locus_tag="VGA15_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX9590279.1"
                     /translation="LVAFPEACEALHSFRAQGGTVILITNAPRPADSVQRQLRKLGVA
                     DAIYDAIVSSGDLTRHFVADHPGRKMFWIGPERDSSIHRGLDPVLAPLEQADYIVCTG
                     LFDDETESAEDYRAMMMQARERQLTLVCANPDIVVERGDRLIYCAGAIAELYRELGGE
                     VIFYGKPHRPIYQRAMALAAERQGHAIPLNRVLAIGDSVRTDLAGAHGFGIDCLFVTR
                     GIHAEDFEGIDQLDPASVKELFGHPPRALTRELRW"
     gene            complement(927..1322)
                     /locus_tag="VGA15_21355"
     CDS             complement(927..1322)
                     /locus_tag="VGA15_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9590280.1"
                     /translation="MAVDLSMSVLVVDDYNTMIRIIRNLLKQLGFEHIDDASDGSAAL
                     NKMRGKKYGLVISDWNMEPMTGYDLLREVRADPNLATTPFIMITAESKTENVIAAKKA
                     GVNNYIVKPFNAATLKTKIEAVFPDTVSA"
     gene            1567..2538
                     /locus_tag="VGA15_21360"
     CDS             1567..2538
                     /locus_tag="VGA15_21360"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX9590281.1"
                     /translation="MTPGFTIIRDTTPAAAIPKGAVVAMGNFDGVHLGHRAVIAAALQ
                     MGHAHRRPALAVTFEPHPRSFFSPNSPQFRLTDETGKLRLLAGTGLAGAVVMTFDKTR
                     AGTTAQDFIHHDLIERLGISGIAVGYDFHFGKGRVGSPSLLVSEAPRLGIEVDVQAHV
                     DIEERPVSSSAIRMALAEGQIADATAMLGGPWFVTGQVIHGEKRGRELGYPTANIRLD
                     KNCGLKHGIYAVRVSRGAERLDGVASFGRRPTFDNGAPLLEIFLFDFKGDLYGAALDV
                     AFIGFIRDELKFDSVDALVRQMDDDSAKARAALVAAPNAFPQLGAIG"
     gene            2535..3104
                     /locus_tag="VGA15_21365"
     CDS             2535..3104
                     /locus_tag="VGA15_21365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEX9590282.1"
                     /translation="MNKTEKQKMLAGELYRPGDAEISADQAATKTWLVRYNAALALPV
                     SERHALLSERLGHVGAGAVIRPPFFCDYGYNIRLGDGVFLNFNCVILDVVEVTIGDRT
                     QIGPAAQIYAADHPRDAKTRRSGLEFGRPVRIGSDVWIGGGAIILPGVTIGDGAVIGA
                     GSVVTRDVGADIIVAGNPARPRQTKPASG"
     gene            3300..6395
                     /gene="ileS"
                     /locus_tag="VGA15_21370"
                     /pseudo
     CDS             3300..6395
                     /gene="ileS"
                     /locus_tag="VGA15_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
     gene            6831..7358
                     /gene="lspA"
                     /locus_tag="VGA15_21375"
     CDS             6831..7358
                     /gene="lspA"
                     /locus_tag="VGA15_21375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX9590283.1"
                     /translation="MPGLERFRNSHLAPGVIAAIAVLALDQASKLWLLFVFDIAHRGA
                     VRVTPFFDLVLAWNVGISFGWFQNDSQIAQIGLMIIKAVAVLVLAIWMARSRTLIATL
                     ALGLIIGGAVGNGIDRFAYGAVVDFALFHLQIGGNTYNWYVFNLADVAIVAGVAALLY
                     DSFLGYPPQKRPDPG"
     gene            7447..8154
                     /locus_tag="VGA15_21380"
     CDS             7447..8154
                     /locus_tag="VGA15_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590284.1"
                     /translation="MRKASGRTVQKSSRALTRVLRLAAVALGIGLLMTAGAVRAQEDD
                     EDDKTFEEKIIEGIMAGIGGTNMENKGIEYRERSPLVVPPKIDLPPPASASADVKAPN
                     WPKDPDEQRRKAAIAARKKDNKDPVEARRTLTPSELNKGKVAASARTNNDPIQPGVSN
                     SPMLSPSQLGFTGSFGDLFGGNKSETAPFKGEPTRDSLTQPPSGYQTPSPNFAYGTGP
                     KESLNKEYNPVAGKYGD"
     gene            8342..>9630
                     /locus_tag="VGA15_21385"
     CDS             8342..>9630
                     /locus_tag="VGA15_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9590285.1"
                     /translation="MMSPHCLAVSLFAALLSTFALSAGGARAQTTVTSERPASFTLGN
                     GLQVVVIPDHRTPVVTQMIWYKVGSADETPGKSGLAHFLEHLMFKGTSKHPAGEFSQT
                     VLRIGGNENAFTSTDYTGYFQRVPREQLAKMMEFEADRMTGLILKDENVLPERDVVLE
                     EFNMRVANNPEARLTEQIMAALYLNHPYGRPVIGWHQEIEKLDREDALAFYRRFYAPN
                     NATVIIAGDVEAKDVRPLAERTFGEVAAQPAIAAQRLRPQEPVPVAPRTVTLADPRVE
                     QPSVKRYYLVPSAATAAAGESPALDVLAQLMGGGSNSYLYRALVVDRPLAVSADAGYQ
                     GTSLDPTQFMISASPKPGVEFAQVEQVIDSVISDVGQNPVRAEDLERVKTQLIAEAIY
                     AQDNQATLARWYGGALTTGLSIDDLRSWPDRIRAVTA"
BASE COUNT         1931 a         3051 c         2940 g         1708 t
ORIGIN      
        1 ggctcgttgc gtttccggag gcctgcgagg cgctgcacag ctttcgcgcc cagggcggca
       61 ccgtgatcct cattaccaac gcgccgcggc cggccgattc ggtgcagcgg caattgcgca
      121 agcttggcgt cgccgacgcg atctatgacg cgatcgtcag ctcgggcgat ctgacccggc
      181 atttcgtcgc cgatcacccg gggcgaaaga tgttctggat cggaccggag cgcgacagtt
      241 cgatccaccg cgggctcgac ccggtgctgg cgccgctgga acaggccgac tacatcgtct
      301 gtaccggcct gttcgacgac gaaaccgaat cggccgaaga ctatcgcgcc atgatgatgc
      361 aggcgcgcga gcgccagctg acgctggtct gcgccaatcc cgacatcgtc gtcgaacgcg
      421 gcgaccgcct gatctattgc gcgggcgcga tcgctgaact ctatcgcgaa ctcggcggcg
      481 aagtgatctt ctacggcaag ccgcaccggc cgatctatca gcgcgccatg gcgctggccg
      541 ccgagcgcca gggccatgct attccgctca accgggtgct tgcgatcggg gattcggtgc
      601 ggaccgattt ggccggcgcc cacggcttcg ggatcgattg cctgttcgtg acccgcggca
      661 tccatgccga ggattttgag ggcatcgatc aactggaccc cgcctcggtg aaggagctgt
      721 tcggccaccc gccgcgggcg ctgacgcggg agttgcggtg gtaggggaac ggtgaggcaa
      781 gcgtgccccg gatgctgcgc agcgcgccgc ccttcgcggc gtggtgcgca gctgaatccg
      841 gggtccatgc tattagcgcc gagtagatcc cggctctgcg gagcagcgtg aaggacgctg
      901 caccgcgtcc gggacacgag cgcgccttac gcgctcaccg tgtccgggaa caccgcctcg
      961 atcttggtct tcagcgtggc ggcattgaac ggcttgacga tgtagttgtt cacgccggcc
     1021 ttcttggcgg cgatcacgtt ttcggtcttg gattccgcag tgatcatgat gaacggggtg
     1081 gtggcgagat tggggtcggc gcggacttcc cggagcaaat cgtagccggt catcggctcc
     1141 atgttccagt cggagatcac cagcccgtat ttcttgccgc gcatcttgtt cagcgccgcc
     1201 gatccgtcgc tggcatcgtc gatatgctcg aagccaagct gcttcagaag atttctgatg
     1261 attcgaatca tggtgttgta atcgtccacc accaggaccg acatcgacaa atcaaccgcc
     1321 atctcgaact ccccacacga cgcttaacgc tgcaaaccat caatcaggct gcgccaggcc
     1381 agaacgcccc gcagtccgca ccccaaagga ctagcatcag ccgttaaaca gcgcgttaac
     1441 cggccgctgc tcctaaaagc agacgcctcc ccccggcgag gccggttccg cccccgcttg
     1501 acttctttgg gcgccccaag tcacggtccc gcccccatcc gatccccgag tcgaagaatc
     1561 gccaaaatga cgcccggctt caccattatc cgcgacacca cgcccgcggc cgctatcccg
     1621 aagggagccg tggtcgccat gggcaatttc gatggcgtcc acctcggtca ccgcgccgtg
     1681 attgcagcgg cactgcagat gggccacgcc caccgccggc cggcgctggc ggtgacgttc
     1741 gagccgcatc cgcgcagctt tttcagcccg aacagccccc agttccggtt gaccgacgag
     1801 accggaaagc tgcgcctgct ggccggaacc gggctcgccg gcgccgtcgt catgaccttc
     1861 gacaagaccc gggccgggac cacggcgcag gatttcattc accatgacct gatcgagcgg
     1921 ctcgggatca gcggcatcgc ggtcggctac gactttcatt tcggcaaggg ccgggtcggc
     1981 tcgcccagcc tgctggtcag cgaggccccc cggctcggca tcgaggtcga tgtccaggcc
     2041 cacgtcgata tcgaggaacg gccggtgtcc tccagcgcga tccgcatggc gctggcggag
     2101 gggcagatcg cagacgcgac cgcaatgctc ggcggcccct ggttcgtgac aggccaggtg
     2161 atccatggcg agaaacgcgg ccgcgagctc ggctatccca ccgccaatat ccgcctcgac
     2221 aagaactgcg gcctcaagca cggcatctat gcggtgcggg tcagccgtgg tgccgagcgc
     2281 ctcgatggcg tcgcaagctt cggccgccgc ccgaccttcg acaatggcgc gccgctattg
     2341 gaaatattcc tgttcgattt caagggcgat ctttacgggg cggcgctgga tgtcgccttc
     2401 atcggcttca tccgcgacga gttgaagttc gacagcgtcg acgcgctggt gcgccagatg
     2461 gacgacgaca gcgccaaggc ccgtgcggca ttggtagcag cacccaatgc gtttcctcaa
     2521 cttggagcga tcggttgaat aaaaccgaaa agcagaagat gctcgccggc gaactttacc
     2581 ggcccggcga tgccgaaata tcagccgacc aggctgcgac caagacgtgg ctcgtgcgct
     2641 acaacgcggc gttggcgctg cccgtttccg agcgtcacgc gctgctgtcc gaacgtctcg
     2701 gtcatgtcgg cgctggagcc gtgatccgcc cgccgttctt ttgcgactac ggctacaaca
     2761 tccgcctcgg cgacggggtg ttcctgaatt tcaattgcgt catcctcgat gtggtcgagg
     2821 tcacgatcgg cgatcgcacc cagatcggac ccgcggcgca gatctacgcc gccgaccatc
     2881 cccgggatgc caaaacgcgg cgcagcgggc tcgaattcgg acgaccggtg cggatcggca
     2941 gcgacgtctg gataggtggc ggcgccatta tcctgcctgg cgtgacgatt ggtgacggcg
     3001 cggtgatcgg ggccggcagc gtggtcacgc gggatgtcgg cgcggacata attgtcgccg
     3061 gaaatccggc ccggccgcgt cagaccaagc cggcgtcagg atgatgccac ttacgcggcg
     3121 gggctttgcg atccggcccc tggctgctat ggaaggccgc atgtttgcgc ggcgcaccat
     3181 catcggcatt agcggcccgg cttccgcctg agctttggct cggcgcaaga ccggggtccc
     3241 actcattcat ccgcgaacga atcgcgtttc agcgcccttt gagccaaccc agagcattta
     3301 tgtccgaaaa gccgcagaag tccgaagcat ccgactattc caataccctg ttcctgccgc
     3361 agacggagtt tccgatgcgc gcggggctgc cgcagcgcga gccggaaatc ctggcacgct
     3421 ggaacgagat cggcctctac ggcaagctcc gcaaagccgc cgccggccgg gcgaaattcg
     3481 tgctgcatga cggcccgccc tacgccaacg gcaacatcca tatcgggcac gcgctgaaca
     3541 agatcctcaa ggacgtggtg accaagagcc agcagatgct cggcttcgat tccaactacg
     3601 tgccgggctg ggactgccac ggcctgccga tcgaatggaa aatcgaggaa gagaactacc
     3661 gctccaaggg ccagccaaag cctgactttc gggactccac cgccatggtg gcgttccgga
     3721 aggaatgccg cgcctatgcc gggcactggc tcaacgtgca gcgcgaggaa ttcaagcggc
     3781 tcggcatcat cggcgactgg gatcatccct acgccacgat ggattatttc gccgaagccc
     3841 agatcgcgcg cgagctgatg aaattcgccg ccaacggcac gctctaccgc ggctcaaagc
     3901 ccgtgatgtg gagcgtggtg gaaaaaaccg cgctggcgga agccgaggtg gagtacgagg
     3961 attataccag cgatacggtg tgggtgaagt ttccgattgc gcaagtcgaa ctcgagtttg
     4021 atccacaagc caccgctaag gatattcagg ctaagcgtca gcttgagagc gacttgaagg
     4081 gaagctcaat tgttatttgg acaacgacac cttggacaat tcctggcaac cgcgcgatca
     4141 gtttttccct cgatatcaac tatgggcttt atgaggttgt cgaagcaccc ccaaataatt
     4201 gggcgaaggt cggtgaccga tatttaatcg ctgatcgttt ggcggatgaa gttttccgac
     4261 aagcaaaaat cgcgagctcc gattataaaa aactgcatga tgttgttttt gatgacggcc
     4321 cggcaagcct cgagataatc tgcgatcatc ctcttaagaa gctcggcggc tatgacttct
     4381 tggtccctct gttagttgga gatcacgtta ctgatgatgc aggtactggt tttgtacata
     4441 ccgcaccagg acatggccgc gaagatttcg atgtttgggt cgcgaacact caacggctac
     4501 tgaagcgcgg cattaatcct aacatcccat acaccgttga tgaaaatggc gcccttactg
     4561 aacaggctcc aggcttcgtg ggaaagcgcg tcttcaccga caagggtgat aagggcgacg
     4621 caaacgaagc ggtcatcaag gcactcgctg acgcagggat gattattgcc cgtttgcgtt
     4681 taaaacatca gtacccgcat tcgtggcgtt caaaaaaacc aatcctctac cgcaataccc
     4741 cgcagtggtt cattgcaatg gacaaagcca ttaccgaaaa ggtttcttca aggctggttc
     4801 acgcaagtaa ggagtcgcca gaatttatcg atgatgaatc gccggatacc cttagaaatt
     4861 gcgcgttaga tgcgatctcg gtcacccaat gggtgccacc gtcgggccag aaccgcatca
     4921 acggcatgat cgatagcaag cccgactggg tgatctcgcg ccagcgcgcc tggggcgtgc
     4981 cgatcacggt gttcgtgcgc gaaaaagccg acggctccgc cgaaatcctg caggacgagg
     5041 ccgtcaacgc gcgcatcgca aaagccttcg agcaggaagg cgccgacgcc tggtacatgg
     5101 acggcgcccg cgagcgtttc ctcggctcgc tcgccaatga agagtggaag aagatcgacg
     5161 acatctgcga cgtctggttc gattccggct cgacccacgc cttcgtgctg gaagaccgcg
     5221 tgcatttccc tggaatcggc gacatccggc gcaaggtcga tggcggcgac gacaccgtga
     5281 tgtatctgga aggctcggac cagcaccgcg gctggttcca gtcgtcgctg ctggagagct
     5341 gcggcacccg cggccgcgcg ccgttcgata tcgtgctcac ccacggcttc acgctcgacg
     5401 agaacggccg caagatgtcg aagtcgatcg gcaacaccgt cgagccgcaa aaggtcatcg
     5461 cgcagtccgg cgccgacatc ttgaggctct gggtctgcgc caccgactac gccgacgacc
     5521 agcgcatcgg cccggaaatc ctcaaaaaca ccatcgagac ctaccgcaag ctgcggaact
     5581 cgatccgctg gatgctcggc acgctgcatc acttccatcg cagcgacatc gtcgcacacg
     5641 ccgagatgcc cgaactggag cggctgatgc tgcatcagct cgccgagcag gccgcgatca
     5701 tgcgcaaggc ttacgccgaa ttcgattaca agaccgtggt cgcctcgctg tcggcgttca
     5761 tgaacaccga actgtcggcg ttctatttcg atatccggaa agatacgctg tattgcgatc
     5821 cgccgtcgtc tctggcgcgt aaggccgccc tcaccgccat cgatattctc tgcgatgcga
     5881 tcctgaaatg gctggcgccg gtattgtcgt tcacctccga agaggcgtgg cggctttaca
     5941 gacccgacgc cgaaccctcg gtgcatctca cgctgtttcc ggaaagtttt gagaatttcc
     6001 gcgacgaaaa gctggcggcg aagtgggaag ttatccgcgc cgtccgccgc gtcgtcaccg
     6061 gcgcgctgga agtagagcgc gccgccaagc acatcggctc gtcgctggag gcgtcgccgc
     6121 tggtctatgt ctcggacaag aagattttcg acacgctgtt cgatatcgat ctggccgagg
     6181 tttgcatcac ctcgaacgcg atggtgacca atgacgacgc gccatcagat gcgttccggc
     6241 tgaacgaggt gcccggcgtc gccgtcgtcg tcgaaaaggc cgttggcacc aaatgcgcgc
     6301 ggtcgtggaa aatcctgccg accgtcggcg acgaccccga ataccctgat gtctcgccgc
     6361 gcgacgcgca ggcgctgcgc gaatggaaag cgctggagca acggccggcg gcgcccgtcg
     6421 ctcccgttcc ggcgcgcgta cgggtggttg aggccgtcgt tgcggaaccc gagccggaaa
     6481 tttccgggca acaagaagcc gagaagcaac cggttgaggt ggccgttccc aggaagagaa
     6541 agcctgccaa gagcaaaagc gaaaaggccg ccggtaaaac cgcgaagcag cccgccgata
     6601 agaagtccgc caaaaagtcc ggcacgacgg gaagcaagaa ggcccccgcc aaaaagaacg
     6661 ccgccaagaa agcgactgcc gcaacgaagg gcgccaagaa gaaacctgca aagaagaaag
     6721 cagcaaccag gaaggctgcc aagaaggggg cgaagaagcc ggcgaagaag gtctccgcag
     6781 cgaaagcaag aaagacgaaa aacaaggcca gcacaaagaa ggccagataa ttgcctggcc
     6841 tcgaacgctt tcgcaactcc cacctcgccc ccggcgtcat cgccgcgatc gccgtgctcg
     6901 cgctcgatca ggcctcaaaa ctctggctgc tgttcgtgtt cgacatcgcc caccgcggcg
     6961 ccgtgagggt cacaccgttc ttcgatctgg tgctggcctg gaacgtcggg atcagcttcg
     7021 gctggttcca gaacgacagc cagatcgccc agatcggcct gatgatcatc aaggccgtgg
     7081 cggtgcttgt gctggcgatc tggatggcgc ggtcgcggac cctgatcgcg acgctggcgc
     7141 tcggcctgat cattggcggt gccgtcggca acggcatcga ccgcttcgcc tatggcgcgg
     7201 tggtcgattt cgccctgttt cacctccaga tcgggggaaa tacctacaat tggtacgtat
     7261 ttaacctggc cgacgtggcc attgttgccg gggtagcggc ccttctgtat gattccttcc
     7321 tggggtaccc gccgcaaaag cgccctgatc ccggctgata cggaccgctt gggctgaacg
     7381 ttgcttgcag gcgcggagtt acgccttgaa ttggcggcct gcggaaacat ttgaacggga
     7441 aaagcgatgc gcaaagctag cggccggacc gtgcagaaat cgtcgagagc attgacccgc
     7501 gtgctgcggc tcgccgccgt cgcgctcggc atcggccttc tcatgacagc cggggccgtc
     7561 cgcgcccagg aagacgacga ggacgacaag accttcgagg aaaagatcat cgaaggcatc
     7621 atggccggaa tcggcggtac caacatggaa aataagggga tcgagtatcg cgagcgctcc
     7681 ccgctggtgg tgccccccaa gatcgatctg ccgccgccgg cctccgcgtc ggccgatgtg
     7741 aaggccccga actggccgaa agatccggac gaacaacgcc gcaaggctgc gatcgcggcc
     7801 cgaaagaaag acaacaagga ccccgtcgaa gcgcgccgga ccctgacgcc gagcgagctc
     7861 aacaagggca aggtagccgc ttccgcccgc accaacaacg accctattca gcccggcgtc
     7921 agcaacagtc cgatgctcag cccgtcgcag ctcggcttta ctggcagctt cggcgatctt
     7981 ttcggcggca acaagagcga gactgcccca ttcaagggcg aaccaacccg cgattcgctc
     8041 acccagccgc ccagcggata tcagacgcca tcgccgaatt tcgcctacgg caccgggcca
     8101 aaagaatcgc tgaacaagga atataacccg gtcgcgggca agtatggcga ttagccacgt
     8161 ccggctctcg gcttcgtgac gctgcagggc agccgcgcgg tgtacggtgg cgcgggttca
     8221 tcccgaacat ttcggttcgg ggggcggtcg ctctcattcc aaatattgcg cgtctcctta
     8281 gtccttgtcg caaaagcaaa tccggtgccc aattttgcgc gaaacacgca ctagacaggt
     8341 catgatgtcc ccacactgcc tcgccgtctc cctcttcgcc gcgctcctat cgacgttcgc
     8401 cctctctgca ggcggcgctc gcgcccagac cacggtgaca tcagagcgtc ccgccagctt
     8461 taccctcggc aacggtctgc aagtcgtggt gatcccggat catcgcacgc cggtcgtcac
     8521 ccagatgatc tggtacaagg tcggctctgc cgacgagacg cccggcaaat ccggcctcgc
     8581 gcattttctc gaacatttga tgttcaaggg caccagcaag cacccggccg gtgaattttc
     8641 ccagaccgtg cttcggatcg gcggcaacga gaatgccttc acctcgaccg actacaccgg
     8701 ctatttccag cgcgtgccgc gcgagcagct tgccaagatg atggagttcg aggcggaccg
     8761 catgaccggc ctgatcctga aggatgaaaa cgtgctgccg gagcgcgacg tcgtgctcga
     8821 ggaattcaac atgcgggtcg ccaacaatcc ggaggcgcgg ctgaccgagc agatcatggc
     8881 ggcgctgtat ctcaatcatc cctatggccg cccggtgatc ggctggcacc aggaaatcga
     8941 aaagctcgac cgcgaagatg cgctggcgtt ctaccgacgc ttttatgcgc cgaacaacgc
     9001 gaccgtgatc attgccggcg atgtcgaggc caaggatgtt cgcccgctgg cggagcggac
     9061 gttcggcgaa gtcgccgccc agcccgcgat agcggcgcag cgcctgcggc cgcaggaacc
     9121 ggtgcccgtg gcgccccgca ccgtgacgct ggccgacccc agggtcgaac aacccagcgt
     9181 gaagcgctat tatctggttc cgtccgccgc cactgccgcc gcgggagaaa gtccggcgct
     9241 cgacgtgttg gcgcagttga tgggcggcgg cagcaattcc tatctttatc gcgcgctggt
     9301 ggtcgatcgc ccgctcgcgg tcagcgccga cgccggatat cagggcacct cgctcgatcc
     9361 gacgcagttc atgatctcgg cctcaccgaa gcccggcgtt gagttcgcgc aggtcgagca
     9421 ggtgatcgac agcgtgatat cggatgtcgg acaaaatccg gtgcgtgccg aggatctcga
     9481 gcgggtcaag acccagctga tcgccgaggc gatctacgcc caggacaatc aggcgacgct
     9541 ggcgcgctgg tatggcgggg cgctcaccac cggcttgagc atcgacgact tgcgaagctg
     9601 gccggatcgc attcgcgccg ttaccgccga
//
LOCUS       DASWGM010000774         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_96712,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000774 DASWGM010000000
VERSION     DASWGM010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96712"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..190
                     /gene="rpmG"
                     /locus_tag="VGA15_21390"
     CDS             23..190
                     /gene="rpmG"
                     /locus_tag="VGA15_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX9590286.1"
                     /translation="MAKAVTIKVKLVSSADTGFYYVAKKNSRTMTDKLVKKKYDPVAR
                     KHVEFRESKIK"
     gene            complement(269..1501)
                     /locus_tag="VGA15_21395"
     CDS             complement(269..1501)
                     /locus_tag="VGA15_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9590287.1"
                     /translation="MPLLQILRPTLPILIGASLMLSLSMGLRQSLGIFLQPLTHDVGI
                     SVSDFTLAIAVQNLAWGFLQPLAGALTVRFGFRTIMMAGAVFYIAGLSLMAGAQGFLS
                     IMLGAGVLIGMSLACTAAAIAMSVATRAVPATVRSTVLGMVSAAGSLGALLSAPLGQV
                     LNEGYGWRTGLVGFVILSLALLPAAWFAGKVDKIPLPPRAPDQIADTSAAVATKIAFG
                     NASFVVMTCAYFVCGMQLVFVTTHLPSYLAICGLDPMLSAQTLGVIGGFNVLGSLFFG
                     WAGERWNKLALLGGIYISRSLVLGWYFMLPPSPTTTLVFGAMMGFLWLGVGPLVAGAV
                     VEMFGLQWQAMIQGLAFMSHQLGSFVGAYGGGLIYDALGSYTMAWRIGVALGLAAGII
                     QVTFALLRPTEPPLVAAR"
     gene            1663..>1797
                     /locus_tag="VGA15_21400"
     CDS             1663..>1797
                     /locus_tag="VGA15_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9590288.1"
                     /translation="MKERILETADRLFYRQGIRAVGVDTVAAEIGISKRTLYNHFPSK
                     N"
BASE COUNT          402 a          573 c          531 g          291 t
ORIGIN      
        1 tttatatttg aggtttgaga acatggccaa agcggtcacc atcaaggtca agctcgtttc
       61 ctcggcggat accggcttct attacgtcgc caagaagaat tcgcgcacca tgaccgacaa
      121 gctggtgaag aagaagtacg atccggtcgc gcgcaagcac gtcgaattcc gcgagtccaa
      181 gatcaagtaa ggttttggca catcgctggc ggatttgaac ggggcctttc ggccccgttt
      241 tgattttgtg gctgtgttgc gaaatgcttt atcgcgcggc caccaacggc ggctccgtcg
      301 gccgcagcaa tgcaaatgtc acctggatga ttcctgccgc aagacccagg gcgacgccga
      361 tccgccaggc catcgtgtag gaaccgagcg cgtcgtagat cagcccgccg ccataggcgc
      421 caacgaagct gccgagttga tggctcatga aggcaagccc ctggatcatc gcctgccatt
      481 gcagcccgaa catctcgacg accgcgcccg ctaccagcgg cccgacgccg agccacagga
      541 aacccatcat cgcgccgaac accagcgtcg tcgtggggga tggcggcagc atgaagtacc
      601 agcccagcac cagcgacctc gaaatataga ttccgcccag cagcgccagc ttgttccagc
      661 gctcgccggc ccagccgaag aacaggctgc ccaggacgtt gaaaccgccg atgacgccga
      721 gcgtctgcgc gctcagcatc ggatccagcc cgcagatcgc cagatacgac ggcagatgcg
      781 tggtgacgaa gacaagctgc atgccgcaga cgaagtaggc gcacgtcatc accacgaacg
      841 aggcgttgcc gaacgcgatc ttcgtcgcga cggcggccga agtatcggca atttggtcgg
      901 gcgcccgcgg cggcagcgga atcttgtcga ccttgccggc gaaccaggcg gctggaagca
      961 gcgccagcga caggatgaca aaaccgacga ggccggttct ccagccgtac ccttcattca
     1021 ggacctgccc tagcggcgcc gacaatagcg cgccgagcga tcccgccgcc gacaccatgc
     1081 ccagcaccgt gctgcgcacg gtcgcgggca ccgcgcgcgt tgccaccgac atcgcgatcg
     1141 cggccgccgt gcacgccaat gacatcccga tcagcacccc cgcgccgagc atgatgctga
     1201 gaaaaccttg cgcgcccgcc atcagcgaga gcccggcgat gtaaaagacc gcaccggcca
     1261 tcatgatggt gcgaaagccg aaccgcaccg tcaacgcgcc ggcgagaggt tgcaggaaac
     1321 cccaggcgag gttttgtacg gcgatggcaa gcgtgaaatc ggaaaccgat atgccgacat
     1381 catgggtcag cggctgcagg aaaatgccaa gtgactggcg caaccccatg ctcaggctga
     1441 gcatcagcga ggcgccgatc agaatcggaa gtgtgggacg aaggatctgc agcaggggca
     1501 tattcttgtt ctccctttgg ctcgccggtc ggcggaccgc ggcctgccat gacgatcagg
     1561 gcttgattac accgatctgt gtaattaagt tatgagccgt tgcattgtca aggaaaaaga
     1621 acacaggtct gtgtaatcat gtcctccccc gcaaagccga ccatgaagga gcggatcctc
     1681 gaaaccgcgg accggctgtt ttatcggcag ggcatccgcg ccgtcggcgt cgataccgtc
     1741 gcggccgaaa tcggcatctc caagcgcacg ctctacaacc actttccctc gaaaaac
//
LOCUS       DASWGM010000775         3627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_767387,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000775 DASWGM010000000
VERSION     DASWGM010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767387"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VGA15_21405"
     CDS             complement(<1..488)
                     /locus_tag="VGA15_21405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX9590289.1"
                     /translation="MTEQKLDRRDFLKTAVVGGAAAAGGSAAIVTPETAQAESVAPAA
                     AAVAPQAAGYAFLNLDEAAFVESLVDHMVPADEISPKGTDLGVNVYIDRALAGAWGKG
                     ERLYMQGPWRQGTPSQGYQLPLTPAQLYRAGIEATNAHCRKVYGKSFDRIEDAQRQEV
                     LVG"
     gene            complement(757..1389)
                     /locus_tag="VGA15_21410"
     CDS             complement(757..1389)
                     /locus_tag="VGA15_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX9590290.1"
                     /translation="MLEAVIWDFGGVLTTSPFEAFARFETERGLPADIIRRTNANNHW
                     ENAWAKFERAEVDIETFDQLFAAESLALGAEVRGKDVLPLLSGDLRPEMVEALRRIGT
                     ELKTGCITNNLPANAIGSLGGRPLYVAEVMVLFDHVIESAKIGLRKPDPRIYRMMVEK
                     LGVDPKACVYLDDLGVNLKPAREMGMTTIKVLNASQAITELETATGLSLR"
     gene            1502..2269
                     /locus_tag="VGA15_21415"
     CDS             1502..2269
                     /locus_tag="VGA15_21415"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX9590291.1"
                     /translation="MKILLLTVAGLALMVCAPAYAIVGGGVPSAEGIGRSVVTIVGSR
                     GNFCSGALIAPKLVLTAAHCVQPGATYKIVEYGAGGQPQLHDVKDVAIHPSFNMQAMM
                     AHRATADVALLQLEAPPKGKNALLLGVPQAPMVAGSRFTIAGIGVTIRGEGKSGGTIR
                     VAGLVATGRPGSLQIRLVDPVGLGARDGLGACTGDSGGPVFEDRQSGPAIVGVISWST
                     GPNGAAGCGGLTGVTPLTLYRDWILQTARQWGVALSM"
     gene            complement(2274..3116)
                     /locus_tag="VGA15_21420"
     CDS             complement(2274..3116)
                     /locus_tag="VGA15_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX9590292.1"
                     /translation="MKARASNLMIGSTTLVAIAAAFVGFLGFQKIHGIAQRGPLRIVF
                     EGSASGLRQGGNVNFDGVQVGEIKSLKLDNPRKVVALAMVDSSAPIRKDTIVGLEFQG
                     LTGVAAISLTGGAAAAPPVPLDWDGIPTLTADLSEVQSIRDTLHSVDRILVGNQAMLK
                     DTLLNFETYTATLASKGDAIDSIIRKADGAFESFDSAMAKIDNIVPGLGSGSADELYD
                     KVRTIRELAESFNKRSGAFMEEGRRSLLDISQAAVKVTRKFDPQTVSGENPPPPWKPN
                     QKRH"
     gene            3188..>3627
                     /locus_tag="VGA15_21425"
     CDS             3188..>3627
                     /locus_tag="VGA15_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590293.1"
                     /translation="MLQAPPIPALLDRAKGQGHFRPKSWLAPYNEKQEYRRKPVPKLL
                     SEDEVADYRDRGYHFPIDALSAGEVADFRRKLEDYEAESGGPIKGEMRHRSHVLFSWI
                     NELVRHPKILDAMEDLLGPNILCWNTSFFIKEAHDPGFVSWHQDA"
BASE COUNT          644 a         1124 c         1138 g          721 t
ORIGIN      
        1 ccgaccagca cctcctggcg ctgcgcgtcc tcgatccggt caaatgattt gccgtagacc
       61 ttgcggcaat gcgcgttggt ggcctcgatg ccggcgcgat aaagctgcgc aggcgtcaac
      121 ggcagttggt agccctgact cggggtcccc tgcctccacg gaccctgcat gtagaggcgc
      181 tcgcccttgc cccaggcgcc ggcaagggcg cgatcgatgt agacgttgac gccaagatcg
      241 gtgcccttcg ggctgatctc atcggcgggc accatgtgat ccacgagact ctcgacaaag
      301 gcagcctcat cgagattgag gaaggcgtat ccggcggctt gcggcgcaac ggcggctgcc
      361 gcgggcgcaa ctgattcagc ctgtgcggtt tccggcgtta caatcgcggc gcttccacct
      421 gccgcggcgg cgccgccgac aacggcggtc ttgagaaagt cgcgccggtc gagcttttgc
      481 tcggtcatga tcccctccct gcctcgggga cgtgcgcccg aaggtcatgc ttttgggagc
      541 gagcatgctg cttttggctc aacccggcaa gacaggattg agatggctgc gatgccgccg
      601 gcgggtaacg cctgacggtt tgtgcaccgc aagcgtgctg gtgttcagtc cacgagagta
      661 tcgaagcgca tctcatccat ccgccgcggc cgcggaagtg gatcaaggat gcgatttaac
      721 gctccagcag atcgcaggga gcaggcgcat cagcgcctat cggagcgaca atcccgtcgc
      781 cgtttcgagc tcggttatcg cttgtgatgc gttcagcacc ttgatggtgg tcatgcccat
      841 ttcgcgcgcc ggtttgagat tgacgcccag atcgtcgagg tagacgcaag ccttggggtc
      901 gacaccaagc ttctcgacca tcatccgata gatacggggg tcgggcttcc tgagaccgat
      961 cttggccgac tcgatgacat gatcgaacag caccatcact tccgcgacgt agagcggtcg
     1021 gccgccgagg cttccgatgg cgttggccgg aagattgttg gtgatacacc cggtcttgag
     1081 ctctgtcccg atacgccgga gcgcctcgac catctcaggc cgcagatcgc cggacagcag
     1141 tggcaaaacg tccttgcctc gaacctccgc gccgagcgca agcgattcgg cggcgaacag
     1201 ttgatcgaag gtctcgatgt cgacttcggc gcgctcgaat ttcgcccagg cattttccca
     1261 atgattattc gcgttggtgc gccggatgat gtcggcgggc agcccgcgct cggtctcgaa
     1321 acgcgcaaac gcctcgaacg gcgacgtcgt aagcacgccg ccaaaatccc agatcactgc
     1381 ctcgagcatc attccccgct catgtgaacg ccgatcggga aacggctagc acggcggctt
     1441 tgcgggggcc agcgtcgatc gccccttggc gaaaaccgtg gtgcgggtta tttctgctcg
     1501 gatgaaaatc ttgcttctga cggttgcggg cctggcgttg atggtctgcg cgcccgccta
     1561 tgcgatcgtc ggcggtggag tgccgtctgc cgaaggcatc ggccgctcgg tcgtcactat
     1621 tgtcggttcg cgcggcaatt tttgtagcgg cgccctgatc gctccgaagc tggtgttgac
     1681 ggcagcacat tgcgtgcagc ccggcgccac ctacaagatt gtcgagtacg gcgccggcgg
     1741 gcagcctcag ctgcatgacg tgaaagacgt ggctattcac ccgtcattca atatgcaggc
     1801 gatgatggcg catcgcgcca ccgccgatgt cgcgctgctg caattggaag ctccgcccaa
     1861 gggaaaaaat gcattgctgc tcggcgtgcc gcaggccccg atggtcgccg gcagccggtt
     1921 cacgatcgct ggaatcggcg taacgatccg gggcgaaggc aaaagcggcg ggaccattcg
     1981 cgtcgctggg ctggtcgcga ccggccggcc gggaagcctg cagattcgcc tcgtcgatcc
     2041 cgtcggactg ggcgcgcgcg acgggcttgg cgcctgcacc ggggattccg gaggcccggt
     2101 gtttgaagac cgccagagcg gccctgccat tgtcggtgtc atcagctggt cgacgggacc
     2161 aaacggcgcc gcgggctgcg gcggtctgac cggcgtcacg ccgctcacgc tttaccggga
     2221 ctggattttg cagaccgccc gccaatgggg tgtggcgctc tcgatgtgag ctttcaatgc
     2281 cgcttttggt tcggcttcca cggcggcggc ggattctctc cgctgacggt ctgcggatcg
     2341 aatttgcggg tcacttttac ggcggcctgg ctgatatcga gcagggagcg gcggccctcc
     2401 tccatgaagg cgcctgaacg cttattgaag ctctcggcca gttcgcgaat ggtcctcact
     2461 ttatcatata gttcgtcggc gcttccgctt cccaggcccg gcacgatgtt atcgattttt
     2521 gccatggcgc tgtcgaagct ctcgaaagcg ccgtcagcct tgcgaatgat gctgtcgatc
     2581 gcgtcgccct tgctcgccaa cgtcgccgta taggtctcga aattcagcag cgtatctttc
     2641 agcatcgcct ggttgccgac gaggatccgg tcgacgctgt gcagggtatc gcggatcgac
     2701 tgcacctcgc tgaggtcggc cgtcagggtc ggaatgccat cccaatccag cggtaccgga
     2761 ggtgccgccg ccgcgccgcc ggtcagcgat atcgccgcca cgcccgtgag cccctggaat
     2821 tccaggccca cgatcgtgtc cttgcggatc ggcgcgctgc tgtccaccat ggcgagggcg
     2881 accaccttgc gagggttgtc gagcttgagc gactttattt cgccgacctg aacgccgtcg
     2941 aagttcacgt tgccgccttg gcgcaggccg ctggcggaac cttcgaaaac gatgcgaagc
     3001 ggaccgcgct gcgcgatgcc atggattttc tggaacccga gaaaaccgac gaacgccgcg
     3061 gcgatggcca caagcgtcgt cgatccgatc atcaggttgc tcgcccgcgc tttcatctca
     3121 agatccggct gcgatgcact tcgtcatcgc ctgattgtac gtgcaaagcc aaccgggcga
     3181 aaggccgatg ctgcaggccc cgcccatacc cgcgcttctt gacagagcca agggccaagg
     3241 tcactttcga ccaaagtctt ggctcgcgcc ttacaacgaa aaacaagaat atcggaggaa
     3301 acctgtgccg aaattattga gcgaggatga agtcgctgat tatcgcgacc gtggctacca
     3361 cttcccgatc gacgcactga gtgccggcga agtggccgat tttcgtcgca agctcgagga
     3421 ctatgaagcc gagagcggag gtcccatcaa gggcgagatg cgccatcgta gtcacgtgct
     3481 gttcagctgg atcaacgagc tggtccggca tcccaaaatc ctcgatgcca tggaggacct
     3541 gctcgggcca aacatcctgt gctggaacac cagcttcttc atcaaggagg cgcatgatcc
     3601 cgggtttgtt tcttggcatc aggatgc
//
LOCUS       DASWGM010000776         7673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_203592,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000776 DASWGM010000000
VERSION     DASWGM010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203592"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(712..2496)
                     /locus_tag="VGA15_21430"
     CDS             complement(712..2496)
                     /locus_tag="VGA15_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9590294.1"
                     /translation="MTYRAPINDMLLALNHGAGLQAAVKAGHYGDFDADITAAVLEEA
                     GRFASDVLAPLNRVGDENGIRLEAGKVTTAPGWPDAYQRWSAAGWNAVSGPEAFGGQG
                     LPLAINAACTEIWSASNIAFGLCPLLTLSAIEALDAHGSIELKKIYLEKLVSGEWPGT
                     MQLTEPQAGSDVGALRTRAERAADGSYRITGTKIFITYGDHDMTDNIVHFVLARLPDA
                     PAGTKGISLFLIPKFLVNADGSLGARNDIHASGIEHKLGMHAAPTCTMTMGDKGGAVG
                     YLIGEENRGMQCMFTMMNQARLGVGLEGVGIADRAYQQALAFAQERRQGRAIGKKGDG
                     SDPIIVHPDVKRMLMQMRALTAAARTICYATAVALDVSVRATDAKVRGDATARGALLT
                     PIAKAFSTDIGNEVSSLGVQIHGGMGFIEETGAAQHLRDARITPIYEGTNGIQSIDLV
                     TRKLAANGGASVWALLDELSGIVEQVEASNDPAFGTTGAKLRDALGSLDRSSRWLLER
                     VTSAPNDALAGATPYLRLFGSTLGGCMLAGEALAARDLGEGAGDPKRYVTLARFFAEN
                     ISVQAGSLERTVIDSAEAVNGADAVLLG"
     gene            2799..2873
                     /locus_tag="VGA15_21435"
     tRNA            2799..2873
                     /locus_tag="VGA15_21435"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2831..2833,aa:Gln,seq:ttg)
     gene            complement(3375..3533)
                     /locus_tag="VGA15_21440"
                     /pseudo
     CDS             complement(3375..3533)
                     /locus_tag="VGA15_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009867220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4127..7348
                     /locus_tag="VGA15_21445"
     CDS             4127..7348
                     /locus_tag="VGA15_21445"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9590295.1"
                     /translation="MRCAKCGATSQEGKRFCADCGAAMLAVVLSEPAPRSVAGSGTVA
                     PTERIQSERRHLTVLFGDLVNSTRLAAERDPEEWRDIATECLEAAGDAIKDLGGYVAR
                     YMGDGVLAYFGWPTASEDDAERAVRAGLTIVKAIVTLNRRLTGKTGSELAVRVGIHSG
                     WVVIDELGTNKVEVFGDTPNIASRVQARCAPNSVLMTGAVHDLVAGQFIVEDCGAHPL
                     AGIERQVQLYRAIAPSGVRRSWRRVGVRGPTPFVNRKREFDVLWSSWLSARNGAGQYV
                     YATGEPGIGKSRLVEEFRSRIKDAHIWMECAGERFSESTPFHAVIKLLEQALGWEKEE
                     SAEGRILQLKRALRLSGLDLSEMMPLIAEMLKLPPSREYPPPQVAPEQARGRLLAGLA
                     EWVLNLAKLQPLILAIEDLHWVDPSSVELIGMLIEQSTDVPLMLMATARPEYHPSWES
                     RATDKSIVLGRLSNDEIGEMIAGSSDIDGLTEEVVAGVVKRSDGVPIFAEELLSFVLE
                     GEGDPTANNIPTTLLDSLTARLDRLGPARRVAQVAAVLGREFDYPLFRAVVPGSDEDV
                     QSALNALIRADLIYTQGRPPKATYQFKHALIRDAAYEGLLKSERRELHARVARTIAEQ
                     FPALAAAQPVLLARHWTEAAEAEPAIAAWKNAGQAALARCAFKEAEEAYRQASASLNR
                     LPPSETRDRGELDLCSALVRVLQVTKGYSAPETMQLGARARVLAERLGDLAQLFRQGA
                     RTWASIFFTGDYAAAASLAEQILEIALTESPNSSHLFFAHNAQVQARFYAGDLAGVED
                     HFAKLSPLLDTMSIAAAADLIVSIGVASRTAWQQGRVDVARERMSRAMDLARKSQDPY
                     AIAMALHFEGNLHWCLKAPRRVEDIANRLLSLSEEHGLGYASDLARVLLGWAKSELGH
                     AAEGVELIKQALAGFAQAGAKVAITYFLTILAQAQACAGDAESGLRTVEEALAANPQE
                     LIWRAYTLTCRGELRLKLGQPAMAEADFRDAIELSRSLEHKAWQLRAATSLARLLMRR
                     GDLRAARGSLVPVYSSFGEGFETPDLQEARLLLVEMAHHLPAT"
     gene            complement(7350..>7673)
                     /locus_tag="VGA15_21450"
     CDS             complement(7350..>7673)
                     /locus_tag="VGA15_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590296.1"
                     /translation="HGCDDVGDVKGLESLGGGGEEDLRDGILTLPASIAIKDPVIAEL
                     FCKPVRSDDELAQISRAVRAAVPAAEERLDQLAAEAKWEAHLHTANPALLYALVDHTR
                     ELSRR"
BASE COUNT         1386 a         2344 c         2457 g         1486 t
ORIGIN      
        1 ctacgagacc ctgagactgg ttcggctgcc ggggctgacg cgtaacctgc cgtgggcgaa
       61 ggcatgagcg cttgtccgag agccggatgc gccaaacgcg caagtccggt tcgatgaggg
      121 gggtgtggaa acagggacca cggctcggcc gctcaggcac cgccagacga aaggggcgga
      181 aacagatacg ccgggcctaa cgttcactgc gccacactcc tactctaccg ttaaaaccat
      241 tgcgtgcggg aatgccgggc gttccggtgt actcgttgtt actcgtgtgc tttctaccaa
      301 tacaaagtgc acacgaggcc gcgggtgcag tgggcacccg gcattcccca cgccctcctt
      361 ggggcggaag attcatcaac ggctcgggcg cattgcgcgg cgaggcgcga acgtgtgttt
      421 taagttggag caaggatggg gatgcgtgcc ccggatgctg cgcagcgcca tcagcgcgtt
      481 cacgcgcgtc ttcgacgcgc cgctctcgcg gcgtggtgcg ctgctgatcc ggggtccgtt
      541 ttgcacagcg caccgtcttg ggtcccggct ctgcggagca gcgtgaagaa cgctgcaccg
      601 cgtccgggac acgaagtcgt atctaaagtt ggctgtttga aaattgaatc aggaatgacg
      661 ttaggatcgc agtcagcgag aagcactagc acggcgctgt gcgacaaccg cctaccccag
      721 caacaccgca tccgcgccgt tcacggcctc ggcgctgtcg atcacggtgc gttccagcga
      781 gcccgcctgc acggaaatat tttcggcgaa gaaccgcgcc agcgtgacat agcgctttgg
      841 atcgccggcg ccctcgccga ggtcgcgggc ggccagcgct tcaccggcga gcatgcagcc
      901 gccaagcgtc gatccgaaca gcctgagata gggggtcgcg ccggccaagg cgtcgttcgg
      961 cgccgaggtg acgcgctcca gcagccatcg gctgctgcgg tcgagcgatc ccagcgcatc
     1021 gcgcagtttg gcgccggtgg tgccgaacgc gggatcgttg gaggcctcga cctgttcgac
     1081 gatgccggac aactcgtcga gcaaggccca gaccgacgcg ccgccattgg cagcgagttt
     1141 tcgggtcacc agatcgatcg actggatgcc gttggtgccc tcatagatcg gagtaatgcg
     1201 cgcatcgcga agatgctgcg cggcgccggt ttcctcgatg aatcccatgc cgccatggat
     1261 ctgcacgcca agcgaggaga cttcattgcc gatgtcggtg gagaacgctt ttgcaattgg
     1321 cgtcagcagc gcgccgcggg ccgtggcgtc accgcgcacc ttcgcgtccg tggcccgtac
     1381 ggaaacgtcg agcgcgaccg cggtggcgta gcagatcgtg cgtgccgccg cggtcagggc
     1441 gcgcatctgc atcagcatcc gcttgacgtc gggatgcacg atgatggggt cggagccgtc
     1501 gcccttcttg ccgatggcgc ggccctggcg gcgttcctgc gcgaaggcca aggcctgctg
     1561 ataggcgcgg tcggcgatgc cgaccccttc gagcccgaca ccaagccgcg cctggttcat
     1621 catcgtgaac atgcattgca tgccgcggtt ttcctcgccg atcaggtaac cgaccgcgcc
     1681 gcccttgtcg cccatcgtca tggtgcaggt tggcgcggca tgcatgccga gcttgtgctc
     1741 gatgccgctg gcgtggatgt cgttgcgcgc gccgagcgag ccatcggcgt tgaccaggaa
     1801 ttttggaatc aggaacagcg aaatgccctt ggtgcccgca ggcgcatccg gcaatcgcgc
     1861 cagcacgaaa tgcacgatgt tatcggtcat gtcgtgatcg ccataggtga tgaaaatctt
     1921 ggtgccggtg atgcgatagc tgccgtcggc agcgcgctcg gcgcgggtgc gcagcgcgcc
     1981 gacatccgat cccgcctgcg gctcggtgag ctgcatggtg ccaggccact cgccggacac
     2041 cagcttttcg agatagatct ttttcagctc aatgctgcca tgggcatcga gcgcctcgat
     2101 cgcactcagc gtcagcagtg ggcagaggcc gaacgcgatg ttcgaggcgc tccagatctc
     2161 ggtgcaggcc gcattgatcg ccagcggcag gccctggccg ccaaacgctt ccggaccgga
     2221 caccgcgttc caccccgccg ccgaccagcg ctggtaggca tcgggccagc ccggcgcggt
     2281 ggtcaccttg ccagcctcga gcctgatgcc gttttcgtcg cccacccgat tgagcggcgc
     2341 cagcacgtcg gaggcaaagc gtccggcttc ctctagcacg gcggcggtga tgtcggcgtc
     2401 gaaatcgccg taatggccgg ctttcaccgc agcttgcagg ccggcgccat gattgagggc
     2461 gagcagcatg tcgttgatcg gcgcgcggta tgtcatatca gtgcctcgct cggggacggg
     2521 agccgcaggc tgtcgtcttc ccacgaaacc gctgcgctct caactctccg atggggtgga
     2581 atcgagccgt gcggcgccgg aggttgcgta ttttcaggcc attgggagct tcggctcgcg
     2641 tgcagaaaca ccttatttgg caagcgacaa gaccctggat cgggttcaac cggaatttct
     2701 ggctctagcc tgttgaaatg gctcaactcc ccctatagac cggcgttgcc ggagggattc
     2761 cgcggaaggc tggtcccgcc gctttccaga gagcttattg gggcgtcgcc aagtggtaag
     2821 gcagtggatt ttgattccac catgcggagg ttcgaatcct cccgccccag ccagcggtgc
     2881 cataactcat tgaatttgtg taactaaccg gtttcgactg gggcgtccga tcgcgattgt
     2941 agcacacgcc tgtacacggg gagcccgcca tatggggaag ctctgcaccg cctgaacgga
     3001 gataatcgga catggcagca aaagtctgtc tccagcgggg gtcgtcgtcg agtgcaagag
     3061 tcgagtgcaa gaacagtgct gttcgtcctc ccaaccttca catcacgccg gacatccgac
     3121 gcgatctcgt cagcgcgcga gcgcgatggg ttccttgtca tgctcgcccc tggccatcga
     3181 gccgctcgct cacctcattt gacgctacca ggccatgatc agacggtcga actgaagaat
     3241 ctgggtattg agcatctgca gttgggcccg agagcggcaa ggcatgcgcg tgccacctcg
     3301 ggtattcgtt tgtcgctcct atccgcaagg acgttgagca gttcctcgac gccgttgcgt
     3361 ccgaccggcg cgacgatccc gaactcagca agatgagcgc ggatcgaatt gattacggca
     3421 gtctgttgac gaacgaacag gtggcgcgtg cgatggagca tcaggcagct ttgctgctcg
     3481 ggcgtcttgg tctcgacgag ccgcatgtta gccctggtga cggcttcgca gatttgccaa
     3541 ggctacaatc ctgggccgcg gcggatcgga gaacactatc agagcgtcgc atttcgcctc
     3601 tgcaattgat gcgaaggcac cgtccaagtc tgcttccttc ctcgccaccg cggcgatcag
     3661 ttcgatcccg atcgaggtcg cagccgtttc agccgtctgg tacatcaagg gatgcgcgac
     3721 atttgaggac attgatacag cgacgcgctt cgcattcctt tttgtcgtgg acgccaccaa
     3781 ccgaccttgg tggcatttcg ctccgcttca agaacgtcag gaggcgtcgt cttaggaggg
     3841 gccacgcgtt cctttctctg gtcactccgg cccgaattcg aagaaacgcc cccgatcacc
     3901 ctttgcaggc gtctcccagt cccgtctctt cagcgatcgc tttcgactat cagaaatcta
     3961 ctttgctcaa taggttggat cgatggtagg atttcctagg caagtacttt tattgaattt
     4021 cgtgttgcta gacagggcca tcccgggcgg gagcgtaacg gtcagcctca cctaaatgat
     4081 gcagtcgtcc gccggtcgcg gatgtatgga atattgcgag cgcgccatgc gatgtgcgaa
     4141 gtgcggtgcg acaagccaag aaggcaagcg cttttgcgcc gactgcggcg cggcaatgtt
     4201 ggctgtcgtt ctgtctgagc cagccccgcg tagcgtcgcc ggttccggca ccgtcgcccc
     4261 aaccgaacgc attcagagcg agcgacgtca cctgaccgtg ctttttggtg acctggtcaa
     4321 ttcgacccgc ctcgcggccg agcgtgatcc tgaagaatgg cgcgacatcg cgaccgagtg
     4381 tctggaggca gccggggatg cgatcaagga tctgggcggc tacgtcgccc gatacatggg
     4441 cgatggtgtg cttgcctatt tcggatggcc caccgcaagc gaagatgatg cagaacgcgc
     4501 ggtccgtgcc ggcttgacga tcgtcaaggc catcgtgacg ctaaaccggc ggcttacagg
     4561 aaaaacaggt tctgagctcg cggttcgtgt gggaatccac agtggatggg tggtcattga
     4621 cgagttgggc accaacaaag tcgaggtctt cggcgatacg ccgaacatcg cgtcgcgcgt
     4681 tcaggcaagg tgcgccccca attccgtttt gatgaccggt gctgtgcacg atcttgtcgc
     4741 gggtcagttc atcgttgagg attgtggcgc acatccgctc gcaggcatcg agcgccaagt
     4801 ccagctttac cgggccatcg cgccgagcgg cgtccgtcgg agctggcggc gcgttggcgt
     4861 ccgcgggcca accccgtttg tcaaccgtaa gcgcgagttc gatgtgttgt ggtcgagttg
     4921 gctcagtgcg cgcaacggcg ccgggcaata cgtttacgcg acgggcgagc cgggaattgg
     4981 aaagtcgcgt ctggtcgagg aattccgctc gagaatcaag gatgcccaca tttggatgga
     5041 gtgcgccggt gaacgcttct ccgagagcac tccgttccat gcggtcatca aattgcttga
     5101 acaagccttg ggctgggaga aagaagaaag cgccgaaggg cggatacttc agctcaagcg
     5161 tgcgctgaga ttgagtggac tcgatctttc ggaaatgatg cctctcatcg cagagatgct
     5221 gaaacttccg ccatcacggg agtatcctcc accccaagtc gcgccggagc aggccagggg
     5281 tcgcctgctg gcgggcttgg cggagtgggt gctgaacctt gcgaaacttc agccgctgat
     5341 cctcgccata gaggatctgc attgggtcga tccctctagc gtcgaactga tagggatgct
     5401 gatcgagcag agtaccgacg tcccgctgat gctgatggcc acggcgaggc cggagtatca
     5461 cccgtcctgg gaatcgcgag cgaccgacaa atcgatcgtc ctcgggcgac tgagcaatga
     5521 cgagatcggc gagatgatcg ccggttcctc ggatatcgat ggtctgaccg aggaggtcgt
     5581 agccggagtc gtcaagcgct ccgatggtgt tccgattttc gccgaagagt tgctgtcgtt
     5641 cgtattggaa ggcgagggag acccgacggc caataatatt cccacgaccc tgctcgattc
     5701 gttgaccgcg cgcttggacc gccttgggcc ggcgcgcagg gtggcgcagg tcgcggccgt
     5761 actcgggcgc gaattcgact atccgctgtt tagggcggtc gtgccgggct cggacgagga
     5821 cgtgcagtca gcgctcaacg cactcatccg ggcggatttg atttacacgc aaggcaggcc
     5881 gcccaaggcg acctaccaat tcaagcacgc attgatccgc gacgctgcct acgaaggctt
     5941 gctcaagagc gaacgccgcg agcttcatgc ccgcgttgcg cgcacgatag ctgaacaatt
     6001 tccggcgttg gctgcggcgc agccggtgct cctggcccgg cattggactg aagcagccga
     6061 agccgagcca gccatcgccg cctggaagaa tgcggggcag gcggcgcttg cgcggtgcgc
     6121 gttcaaggaa gcggaggagg cctatcgtca ggcaagcgcg tcgctcaatc ggctgccgcc
     6181 gtcggaaacg cgcgacaggg gcgagctgga cctttgcagc gcgctcgtgc gcgtgctcca
     6241 ggtgaccaag ggctattccg cgccggagac gatgcagctt ggggcgcgtg cacgggtgct
     6301 cgcggaacgg ctcggcgacc tcgcgcagct ctttcgccag ggcgcccgaa cctgggcctc
     6361 catcttcttc acgggagact acgcggccgc cgcctccctt gccgagcaaa tcctcgaaat
     6421 tgctttgacc gaaagcccga attccagcca cctgtttttc gcacacaacg cccaagtgca
     6481 ggcgcgattc tacgcagggg accttgccgg agttgaagat cactttgcga agctctctcc
     6541 gttgctcgac actatgtcga ttgccgctgc agccgacctc atcgtttcga tcggggtcgc
     6601 gagccgtacc gcgtggcagc agggccgtgt cgatgtcgcg cgcgagcgga tgtctcgcgc
     6661 gatggatctg gccaggaaga gtcaggaccc ctatgcaatc gcgatggcgc tgcacttcga
     6721 gggtaatctg cactggtgcc tcaaggcgcc gcggcgggtc gaggatattg ctaatcggct
     6781 gttgtccctg tcggaggaac acgggctcgg ctatgcctcg gacctggcgc gcgtgctact
     6841 tggctgggcc aaatcagaac tcggtcacgc cgctgaaggc gtggaattga tcaagcaggc
     6901 cttggccggc ttcgcccagg caggcgcgaa agtggctatt acctattttc tcacgattct
     6961 ggcgcaggca caggcgtgtg ccggcgacgc cgaatcaggc ctccgcactg tcgaggaagc
     7021 cctcgccgcg aatcctcagg aattgatttg gcgcgcatat accctgacat gccgcgggga
     7081 gctgcggctc aaactggggc agccggcaat ggccgaggct gatttccgcg acgcgatcga
     7141 actgtcgcga tcactcgagc acaaagcctg gcagctccgt gctgcgacaa gccttgcacg
     7201 gttgctgatg cgacgcggcg atctccgcgc cgcgcgggga agtctcgtgc ctgtctattc
     7261 cagcttcggc gaaggtttcg agacgcccga cctgcaggaa gccagattgc tcttggtgga
     7321 gatggcgcat catctacccg cgacttgacc taacggcgcg acagctccct ggtatggtcg
     7381 acaagcgcgt acagaagcgc cggattggca gtatgcagat gcgcctccca cttggcctcg
     7441 gccgccaatt ggtcgagtcg ttcctcggcg gccggtaccg ccgccctgac cgcccgagag
     7501 atttgcgcca gctcgtcatc cgatcgcacc ggcttgcaga acagttctgc gatcaccggg
     7561 tctttgatgg caatcgaggc gggcagcgtg aggatcccat ccctcaaatc ctcttcgccg
     7621 ccgccgccga gcgattccag tcccttgaca tcgcccacgt cgtcgcaccc gtg
//
LOCUS       DASWGM010000777         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2603624,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000777 DASWGM010000000
VERSION     DASWGM010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2603624"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VGA15_21455"
     CDS             complement(<1..219)
                     /locus_tag="VGA15_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590297.1"
                     /translation="MRSIVSLTLAACAIAVMSSPALAQAQAQAQTPAPAAAPATPPAP
                     PSAGGTPDAMPFDIPYGQSIGLEHAKQVM"
     gene            complement(276..893)
                     /locus_tag="VGA15_21460"
     CDS             complement(276..893)
                     /locus_tag="VGA15_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590298.1"
                     /translation="MSGTTQRTLAALSLALICIASPASAADDTVRIRGTIERVEGPVY
                     VVKNRDGAELKLTVTDNPLFVAIAPSTMADIKPGMFVGSAGTMQPDGTQKAIEVHIFP
                     ESMRGTGEGHYDWDLKPQTKMTNGNVEQTVAGVDGPLLSVKYKDGEKKLVVTPETVVV
                     TYVPGNKDDLKPGTRIFVGAAKKQPDGTLQTPRITYGRNGAGPAF"
     gene            complement(1326..2315)
                     /locus_tag="VGA15_21465"
     CDS             complement(1326..2315)
                     /locus_tag="VGA15_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9590299.1"
                     /translation="MPFEPNAKTASYLFALAAWMLAALATDLAAASYPDRTIKIVVPL
                     APGGGTDLISRTLAQEMAKDLGASVIIENKPGAGTIIGSQAVATSEPDGYTLLMGTFA
                     NAVNPSLNAKLPYDPHRDFAAVALIARAFNIVVVNPKSPVQSVADLIAAAKAEPDKLS
                     YGTYGNGTSAHLAGELFKSLAKVNLTTVPYKGSAPAITDLIGGQIQVMFTTVASAASL
                     IAGGQLRALAVTSAERSPAFPQLPTVAEAGVPGYAVESWYGLFAPARTPPDVIDRLNK
                     SAALAVQSEAFKKLGVNEGLVMVARPPQELDRYFRGEEERWRKVIQDAGIKVE"
     gene            2495..>3279
                     /locus_tag="VGA15_21470"
     CDS             2495..>3279
                     /locus_tag="VGA15_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEX9590300.1"
                     /translation="MKHWLIGLLLLIAPSAFAQQAAPTIAFDSVPNPLRLPPNMYFGE
                     VSGVSVNSKGHVFALSRGNTSGPAYAAAATQLLEFDAKGAFVREIGKNLYAWSFGHTV
                     KIDPADNIWVTDKGSDMVIKFDPEGRVVMVFGRKQEASDEETGPVKHPKPPLPAEDGR
                     FRQVTDVAWDKAGNTFISDGYINSRIAKVDKDGNWLKSWGDRGKEPGQFNTPHSIATD
                     AKGNVYVADRGNHRIQVFDGDGKFLRQFRIDVPVPPDAKPAIG"
BASE COUNT          633 a         1005 c          990 g          651 t
ORIGIN      
        1 catcacctgc ttggcgtgct cgagcccgat cgactgaccg tagggaatat cgaacggcat
       61 cgcatcgggc gtaccgcccg cggacggcgg cgccggcggc gttgctggcg ctgccgccgg
      121 cgctggcgtt tgtgcttgtg cttgtgcttg cgcaagtgcc ggcgaactca tgaccgcaat
      181 ggcgcaggct gccagtgtca gcgaaacgat tgaacgcata gtatcctcca ggttgaagta
      241 aagactgtcg acggtgccgc gacgctatat ctaagtcaaa atgccggacc cgcaccgttt
      301 ctgccgtaag tgatacgcgg ggtctgcaac gtgccgtcag gctgcttttt cgcggcgccg
      361 acgaagattc tggttcccgg ctttagatcg tccttgttgc cggggacata ggtgacaaca
      421 acggtttccg gggtcaccac cagtttcttc tcgccgtcct tgtacttgac ggacagcagc
      481 ggtccatcga ccccggcaac cgtctgctcg acattgccat tggtcatctt ggtctgcggc
      541 ttgagatccc agtcgtaatg gccttcgccg gtgccgcgca tcgattccgg gaagatatgc
      601 acctcgatcg ccttctgggt gccgtcgggc tgcatcgttc cagccgagcc gacgaacatg
      661 cctggcttga tgtcggccat ggtcgaggga gcgattgcga caaacagcgg attgtcggtg
      721 acggtcagct tcagttcggc accgtcacgg ttcttgacca cgtaaacggg accttccacg
      781 cgttcgatgg tgccgcggat gcggacggtg tcatccgccg ccgacgctgg cgacgcgatg
      841 cagatcagag cgaggcttag ggcagcaagg gtgcgctgtg tggtgccgga cattgttgat
      901 cctccctgga ttttcgaatt gacggttaac tcattcgtta ttccatcggt ggcctattcc
      961 ccagatgctt gtcgaagaag ccggcgaaca tcgccgcacc agcggtttgg gcatgcccag
     1021 cgggcgaccc gaccattcct atcgcagcgc acaaaagcgc tgtggtgccc gattttcagg
     1081 tcgactttga tccacgtcaa cttgcatgtg acaggaatat gaattccgtt ctccggtatc
     1141 ctcatcgcgt tccccctgtt cagtcttttt cttgattctt caaagagtag tttcttgatg
     1201 cttcaaaaga gtagtgcggc ggattgcgca cgacggacgc ccctctgcaa cacccgcgct
     1261 ccgcgaatat tcctgcaagc cggcaacaaa ctttcgatcg cggccgaccg caacccgcgc
     1321 ccccgctatt cgaccttgat cccggcgtcc tggatcacct tgcgccagcg ctcctcctcg
     1381 ccgcgaaaat atcgatcgag ctcttgcggg ggtcgtgcga ccatgaccag gccctcattc
     1441 acgccgagct tcttgaaagc ctcggattga accgccaagg ctgcagattt gttgagccgg
     1501 tcgatgacat cgggaggcgt cctggccggc gcgaacaggc cataccagct ctccacggcg
     1561 tagccgggga cgccggcctc cgcgacagtg ggcaattgcg ggaatgcggg cgaacgctcc
     1621 gccgaggtca cggcaagcgc acgcaactgg ccgcccgcga tcagcgaagc cgcgcttgca
     1681 accgtcgtaa acatcacctg gatttggccg ccgatcaggt cggtgatggc gggcgccgag
     1741 cccttgtagg gcaccgtcgt cagattgacc ttggccaggc tcttgaacag ttcgcccgcg
     1801 agatgcgccg aggtgccgtt gccgtacgtc ccataggaca atttatcggg ttcggctttc
     1861 gccgcggcga tcaaatcggc aaccgattga acgggggatt tggggttgac cacgacaata
     1921 ttgaaggcgc gcgcgatcag cgcaacggct gcgaaatccc tgtgcgggtc gtaaggcagc
     1981 ttcgcgttca ggctcggatt gacggcgttc gcaaacgtcc ccatcagcag cgtatagcca
     2041 tccggctcgc tggtcgcgac tgcctgactt ccgatgatgg ttcccgctcc aggcttgttc
     2101 tcgataatga ccgacgcgcc gagatccttc gccatctcct gcgccagcgt gcgcgaaatc
     2161 aagtcggtgc cgccgccagg cgctaacggg acgacgattt tgatggtccg gtccggatat
     2221 gaggccgcgg ccagatcggt cgcaagagca gcgagcatcc atgctgcgag cgcgaacaga
     2281 taagacgctg tctttgcatt gggctcaaat ggcatggcac ctgccggcaa actaaagatt
     2341 cccgaaacac cgtagcagat tattcactcc ggcgcagcgc tatgcgccgg atgacgaacg
     2401 gttatgactg gggatgcgcg tgcaaggatg gacgccaggt ctcgcgatag actagcatcc
     2461 atcccaaaga caagaacact ccggggagga aaagatgaag cattggctga tcgggctgtt
     2521 gctgttgatc gcgccatcgg ccttcgcgca gcaggcggca cccaccatcg cttttgactc
     2581 ggtcccgaat ccgctccggc tgccgcccaa catgtatttc ggcgaagtgt cgggcgtctc
     2641 ggtcaattcc aagggacacg ttttcgcact ctcgcgcggc aacacctccg ggccggccta
     2701 cgccgcggcg gcgacgcagt tgctggaatt cgatgcgaag ggcgcgttcg tccgcgaaat
     2761 cggcaagaac ctctatgcct ggtcgtttgg gcacacggtg aagatcgacc ctgccgacaa
     2821 catctgggtg accgacaagg gctccgacat ggtgatcaag ttcgatcccg agggccgggt
     2881 cgtcatggtg ttcggccgca agcaggaagc ctccgacgaa gagaccggac cggtcaagca
     2941 tccgaagcca ccgctgcccg ccgaggatgg ccggtttcgt caggtcaccg atgtcgcgtg
     3001 ggacaaggcc ggcaatacct tcatcagcga cggctacatc aactcccgca tcgccaaggt
     3061 cgacaaggac ggcaactggc tgaagtcatg gggcgaccgc ggcaaggagc ccggccagtt
     3121 caacaccccg cactccatcg cgaccgacgc caagggcaat gtctatgttg ccgatcgcgg
     3181 caaccatcga attcaggtgt tcgacggcga cggaaaattt ttgcgccagt tcaggatcga
     3241 cgtgccggtg ccgccggacg caaaacctgc gatcggcaa
//
LOCUS       DASWGM010000778         4985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1774205,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000778 DASWGM010000000
VERSION     DASWGM010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774205"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VGA15_21475"
     CDS             complement(<1..146)
                     /locus_tag="VGA15_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX9590301.1"
                     /translation="MSALISVTPDNVETVLSDLPRLVRLAFGFGSKLRRGTLDVTLPD
                     GRTLR"
     gene            complement(230..1138)
                     /locus_tag="VGA15_21480"
     CDS             complement(230..1138)
                     /locus_tag="VGA15_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEX9590302.1"
                     /translation="MAQQTARSEAHRGTEDAAAALYFGDVMHARLKPIGHRFSYRVMS
                     LMVDLDRLDAADRQSRLFGVNRAALYSFNEADHGERDGSALRIYAQRCAAEHGIDLTG
                     GRVLLLCYPRLLGYTFNPLSTYFCYRADGELALMIYEVRNTFGDIHAYVLPVKPGESS
                     DAGVRQAQEKLFYVSPFIGMAMRYHFRVSPPGDSVKLRILETDHDGPLLAATFNGRRR
                     ALTTAALLRSFCSLPLVTLKIVAAIHWEALRLWLKGARLVPRPNTVPARPVNTVLATG
                     ECNDYSGTALSAVGREPGLRESAPAR"
     gene            complement(1141..2454)
                     /locus_tag="VGA15_21485"
     CDS             complement(1141..2454)
                     /locus_tag="VGA15_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9590303.1"
                     /translation="MRVAVIGTGIAGNAAAWTLSKHYPVTVYDREIRPGGHSHTVSID
                     YDGTPLSVDIGFIVYNELNYPELTALFAHLDIETVESCMSFAVTADDGRFEWKGGGRS
                     WRETAGGLFAQPRNLLSPSYLRMLRDILTFNAQGVADHAAGRLAGLTLAEYFRDRKFA
                     PRLLTDYLAPIGAAIWSAPASGMLDFPAENFVAFFSNHRLLQYDRPVWRTVKGGSQRY
                     VAKLASAFRDRLRLGCAVTSIRRTPHGVVVNDSHGGSDSYDHVVIASHSDQALKMLSD
                     ADDRERAVLGAIGYSPNTVYLHRDVGLMPKRRRAWASWNFLRWQREGAAENDVAVTYW
                     MNRLQGIDDDKPLFLSLNPPFAPACELTFGKYSCEHPQYDAAAFAAQKRLGEIQGRRQ
                     TWFCGAWTGYGFHEDGLRSGLAVAQALGAAVPWRGPPPELAQAAE"
     gene            2898..4385
                     /locus_tag="VGA15_21490"
     CDS             2898..4385
                     /locus_tag="VGA15_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX9590304.1"
                     /translation="MLFGAVDGLPAAIPCLDRAARWLAALCIVVSAAGCIVTQDLPDP
                     ALDIPQAYKPQDYKAAKVAEQNAPPTLDWWRGFRSSELTDLMEEAQKVNLDIAAATAR
                     FIQADAQARVAGAALLPSLSGNGQETYSRTSGSSASGLTNGGREVVNYSASLSASYEL
                     DFWGKNRDAAQAAEETSVANRFDRDVVALTTLTSVANAYFQVLASQDRIRTAERNIAS
                     ATRILEAIRQRFKAGTGTDLDVAQQEAVLANQRASVPPLRQTLSQNINALATLVSRPP
                     ESLHVVGGSMNQISSPRVTPGLPSELLTQRPDIRRQEAQLASAVANVGNARAQFFPSI
                     QLTGQGGYQSSALVALFQPHAAFFSLVGGLTQPIFDGGKILGNFEFTKARQDELLQTY
                     RKTVVSAFADVDNALVAIRQTTEKLRLQREVVAASQRAFQLSEQQLRAGTADIVTVLN
                     TQLTLFQAEDVLWQAQLARLLAIVSLYQALGGGWEPRMEKPVNAL"
     gene            4375..>4985
                     /locus_tag="VGA15_21495"
     CDS             4375..>4985
                     /locus_tag="VGA15_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX9590305.1"
                     /translation="MLFKPDLNEVAKTAGDRVARVASRARRRTVSIAITLLILGGLGY
                     IGWTALQQKQQAANRAAARPDLPVPVLAATPRILDVPVYLDGVGSVRALNNVLVRAQV
                     DGKLIAVNFTEGQDVKKGDVLGEIDPVLYKAQLDQAIAKKAQDEALLANQRLDLARYQ
                     QLAASNAGSKQQADTQRATVAQTEALVNSDQAAIDNQQAMLGYT"
BASE COUNT          973 a         1583 c         1552 g          877 t
ORIGIN      
        1 cgcagcgtac gtccatcggg aagcgtcaca tcgagcgtac cgcgccgcag cttcgagccg
       61 aatccgaacg ccagtcgtac aagacgcggc aaatcggaaa gtaccgtttc gacattgtcg
      121 ggggttacag aaatcaacgc agacatttac agacccatct gcagacccat cgactgatgt
      181 ggctctgtgt ccgagcttcg tatgggccgg cttgcaccag atccgaccgt caccgggccg
      241 gagcgctttc ccgcaatccg ggctcgcgcc cgaccgccga taacgccgtc ccactataat
      301 cgttgcattc gccggtcgcc aagacggtat tcacgggcct cgccggtacg gtgttggggc
      361 gcggcaccag acgcgcgcct ttgagccaga gccgcagcgc ttcccaatgg atcgccgcga
      421 cgattttcaa ggtgaccagc ggcagggagc agaacgaccg caacagcgcc gcggtagtca
      481 gggcgcgtcg gcggccgttg aaggtcgcgg cgagcagggg gccgtcgtgg tcggtctcca
      541 gaatgcgcaa cttgacgctg tcacccggcg gcgacacccg gaaatgatag cgcatcgcca
      601 tcccgataaa gggagagacg taaaacagct tttcttgcgc ctgccggaca ccggcgtcgc
      661 tgctctcgcc tggcttcacg ggcaacacat aagcgtgaat atcgccaaac gtgttgcgaa
      721 cctcatagat catcagggcg agctcgccgt cggcgcgata gcagaaatag gtcgacaacg
      781 gattgaaggt gtagccgagc aggcgcgggt agcacagcag caacacgcgc ccgccggtga
      841 ggtcgatgcc gtgctccgcc gcgcaacgtt gcgcgtaaat gcgcaaagct gacccgtcgc
      901 gttcgccgtg atcggcttcg ttgaaactat agagagcggc ccggttcacg ccgaacaacc
      961 gcgactgccg gtccgccgcg tcaagccggt cgagatcgac catgaggctc atcacgcgat
     1021 agctgaagcg atggccgatc ggcttcagac gcgcatgcat cacgtcgcca aaatagagcg
     1081 cggccgcggc gtcttcggta ccgcgatgtg cctctgatct ggcggtttgc tgggccatcg
     1141 ctactccgca gcttgcgcca gttcaggcgg tggtccgcgc cagggcaccg ctgcgcccag
     1201 cgcttgcgca accgccagtc ccgagcgcaa tccatcctcg tggaaaccgt atccggtcca
     1261 ggcgccgcag aaccaggttt gacgtcgccc ctgaatttcg ccgaggcgtt tctgcgccgc
     1321 aaacgccgcg gcgtcatatt gcggatgttc gcagctatat ttgccgaacg tcagttcgca
     1381 agcgggcgcg aacggcggat tgaggctgag gaacaacggc ttgtcgtcat cgatgccttg
     1441 cagccgattc atccagtagg tcacggcgac gtcgttttcc gctgcccctt cgcgctgcca
     1501 gcgcaggaaa ttccacgacg cccaggcccg cctgcgcttc ggcatcaatc cgacgtcgcg
     1561 atgcaggtag acggtgttgg gcgaatagcc gatggcgccg agcacggcgc gttcgcggtc
     1621 atcggcgtcc gacagcatct tgagcgcctg atcgctgtgc gaggcgatca cgacgtgatc
     1681 gtaactgtcg cttccgccgt gactgtcatt gacgacgacg ccgtgcggcg tccgccgtat
     1741 cgatgtcacg gcgcagccga gccgaagccg atcccggaag gcggacgcga gcttcgccac
     1801 gtagcgctgg ctgccgccct tcaccgtgcg ccagaccggg cgatcatatt gcagcaggcg
     1861 gtggttgctg aaaaaggcga cgaagttttc ggcgggaaag tccagcatcc cgctggccgg
     1921 tgcggaccag atcgcggctc ctatcggcgc aagataatcg gtcagcaacc ttggtgcaaa
     1981 tttgcgatcg cgaaaatact cggcgagggt aagtccggcc agtctgcccg cggcatggtc
     2041 cgcaacgcct tgcgcgttga aggtcaggat gtcgcgcagc atccgcaagt atgacggaga
     2101 caacaggttt cgcggctgcg caaacaagcc tcccgcggtt tcgcgccagc tgcgtccgcc
     2161 gcccttccat tcgaagcgtc catcgtccgc cgtgaccgca aagctcatgc agctttccac
     2221 ggtctcgata tccagatggg cgaacagcgc cgtcagctcc ggatagttca attcattgta
     2281 gacgataaag ccgatatcga ccgaaagcgg tgttccatca tagtcgatgg agacggtgtg
     2341 gctgtgcccg ccgggcctga tttcgcgatc gtagaccgtg accggataat gtttcgacaa
     2401 ggtccaggcc gcggcattgc cggcaattcc cgttccgata accgcgacgc gcattcgaaa
     2461 gcccccgtgc ctcgatgggc gagatacggg gctttgcgag cgaagtttca gtgcggcggc
     2521 cgcaggggcg gggcaggacg aatcaattgg cctgacgagt gaaacaacat gaaatcttgg
     2581 taacatgaaa tcttggtaag atggccgggc tgctcgaatc gaacagatca gcagaaagcc
     2641 ccatagaata tagaaaactc ggctttggcc gtcccggtcg gtaccctctg ctcaggcgaa
     2701 agcgcgggga cagcgggcgg aacgggctgt ccgcgcaatg ttcccccggc tagaatggca
     2761 ggggttccca gaggaagcat cacaccggtg aacgtctcgc agccagtctc cacaggagtc
     2821 atccgcgaat cggcctggcg gttctcgcag cgtcggatgc cgggaactat gcccggcgga
     2881 gggcagatgt gagacagata cttttcggtg ccgtcgacgg gctgcctgct gccatccctt
     2941 gcctggatcg cgccgcgcga tggctggcgg cactttgcat cgttgtcagc gccgccggct
     3001 gcatcgtgac ccaggatttg cctgatccgg cgctggacat cccgcaagcc tacaagccgc
     3061 aagattacaa ggccgcgaag gtcgcggagc agaacgcgcc tccgacgctg gactggtggc
     3121 gcggcttccg ttcgtccgaa ttgactgatc tgatggagga ggcccagaag gtcaatctgg
     3181 acatcgccgc ggcgacggca cgcttcattc aggcggacgc gcaggcacgt gttgcaggcg
     3241 cggcgctgct gccgagcctc agcggcaacg gtcaggaaac ctattcccgg acatccggct
     3301 caagcgccag cggattgacc aatggtgggc gcgaggtcgt caactattca gcctcgctca
     3361 gtgccagcta tgaactcgat ttctggggca agaaccggga cgccgcgcag gccgccgagg
     3421 aaacctccgt cgccaatcgt ttcgatcgcg acgtggtcgc ccttacgaca ctcaccagcg
     3481 tcgccaatgc ctattttcag gtactcgcct cgcaggaccg gattcgcacc gccgaacgca
     3541 atatcgccag cgcgacacgc atcctcgagg cgatcaggca acggttcaaa gccggcacag
     3601 gaacggatct cgatgtggcc caacaggaag ccgttctcgc caatcagcgg gcctccgttc
     3661 cgccgctgcg gcaaaccctg tcgcagaaca tcaatgcgct ggcgacactg gtgtcgcgcc
     3721 cgccggaaag cctgcatgtc gtcggcggtt cgatgaatca aatctcctcg ccgcgagtga
     3781 ctccgggctt gccatcggaa ttgctgacgc agcgtccgga tatccggcgc caggaggcgc
     3841 agctcgcgtc cgctgtcgcc aatgtcggca acgctcgcgc acagtttttc ccaagcatcc
     3901 agctgaccgg gcagggcggc taccaaagct cggcactggt ggcgctgttc cagccgcatg
     3961 ccgcgttctt cagcctggtc ggcggcctga cgcagccgat tttcgacggc ggaaaaattc
     4021 tcggcaactt cgaattcacc aaggcccggc aagatgaatt gctgcagacc taccgcaaga
     4081 cggtggtctc cgcgttcgct gacgtcgaca acgcgctggt tgcgatccgc cagaccaccg
     4141 agaagctgcg gctgcagcgc gaggtggtgg cggcctcgca gcgagccttt caactttccg
     4201 agcagcagct tcgtgccggg accgccgaca tcgtgactgt gctaaatacg caattgacgc
     4261 tatttcaggc cgaagacgtg ttgtggcagg cccagcttgc ccggctgctc gcgatcgtca
     4321 gcctgtatca ggcgctgggg ggcggctggg aaccgagaat ggagaagccg gtcaatgctc
     4381 tttaagccgg atttgaacga agttgcgaag accgccggcg atcgtgtggc ccgcgtcgcc
     4441 tcccgcgcgc gccgtcgcac cgtttcgatt gcgataacgc tgctgatcct tggcggcctc
     4501 ggctacatcg gctggacagc gctgcagcag aaacaacagg ccgcgaaccg ggccgccgcg
     4561 cgtcccgatc tccccgtgcc cgtgctggcg gcgacaccgc gcatcctgga cgtgccggtc
     4621 tatctcgatg gcgtcggttc cgtccgcgcg ctgaacaacg tgctggtgcg cgctcaggtc
     4681 gacggaaaat tgatcgcggt caatttcacc gagggacagg acgtcaagaa gggcgacgtt
     4741 ctcggcgaaa tcgatcctgt gctctacaag gcccagctcg accaggcgat cgccaagaag
     4801 gcgcaggatg aagcgctgct cgccaatcag cgcctcgacc tcgcacgcta tcagcagctt
     4861 gccgcgtcca acgccggttc caagcagcag gccgataccc aacgcgccac cgtcgcccag
     4921 accgaggcgc tggtgaattc cgatcaggcc gcgatcgata accagcaggc aatgcttggc
     4981 tatac
//
LOCUS       DASWGM010000779         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2316022,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000779 DASWGM010000000
VERSION     DASWGM010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316022"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGA15_21500"
     CDS             complement(<1..576)
                     /locus_tag="VGA15_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9590306.1"
                     /translation="MKHFNLSAWAVSHPALILFLVVALGAAGFVSYQKLGRAEDPFFT
                     VKVVNVSAIWPGATAAEMQTQVADPIEKKLQELPFFEKVQTYSKPSFTAMQVTFKDST
                     PPKDVPYLFYVLRKKLADVQGQLPANLLGPVVNDEFSDVDSILYMMTGDGADYVQLKK
                     AAEGMRQRLLKVPGVTKVDLYGTQDERIYVEF"
     gene            complement(592..>1515)
                     /locus_tag="VGA15_21505"
     CDS             complement(592..>1515)
                     /locus_tag="VGA15_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9590307.1"
                     /translation="TIKPRIETDMGFRVPGKVAKRLVEVGQTVDVGQPLATLDEVDLK
                     LQAEQADAEFHAATGVLAQAAAAETRAKDLRVKGWTTDAQLDQAKAAGDEARARLNRA
                     ERSVELTKNSLSYATLVADTRGVVTATLIDAGQVVASGQTAIRVARFAEKEAVVSIPE
                     TLVGRAKDGAASVTLWSEPDKKYAAKLREVAPSADPATRTYLAKFSLPDAGETVSLGM
                     TATLTLTDPATTRVARLPLSALFSQGGDPSLYIVDDKGEVALKPVTVKSYESNCVVIS
                     GGVDEGAKVVALGVQKLDPAQKVRVVSSLSF"
BASE COUNT          264 a          471 c          480 g          300 t
ORIGIN      
        1 gaactcgaca tagattcgct cgtcctgggt gccgtagaga tcgacttttg tcactcccgg
       61 taccttcaac aggcgctgac gcataccttc agctgccttc ttgagctgca cgtagtcggc
      121 gccatcgccg gtcatcatat aaaggatcga atcgacgtcg ctgaattcgt cgttgacgac
      181 gggaccgagc agattggccg gaagctgccc ctgcacgtcg gccagcttct tgcgcagtac
      241 ataaaacaga tacggcacgt ccttgggcgg cgtcgaatcc ttgaaggtga cctgcatcgc
      301 ggtgaacgac ggttttgaat aggtctggac cttttcgaag aacggcagtt cctgcagctt
      361 cttctcgatg gggtcggcga cctgggtctg catctcggct gccgtagcac ccggccagat
      421 cgccgacacg ttcaccactt tcacggtgaa gaatggatct tcggcgcggc cgagtttttg
      481 gtaggagacg aacccggcgg cgcccaaggc gacgaccagg aacagaatga gcgccggatg
      541 gctgaccgcc caggccgaaa gattgaagtg tttcatcacg ccctcctcag ctcaaaacga
      601 caatgacgag acgaccctga ccttctgggc cggatcgagt ttctgcacac cgagcgcgac
      661 aaccttggcg ccttcatcga cgccgccact gatgacgacg caattgctct cataggattt
      721 taccgttacc ggcttgagcg ccacctcgcc cttgtcatcg acgatgtaga gcgagggatc
      781 gccgccctgg ctgaacagcg ccgacagcgg cagccgcgcc acgcgggtgg tggcgggatc
      841 ggtcagcgtc agcgtcgcgg tcatgccgag cgagacggtt tcgccggcgt caggcagcga
      901 gaattttgcc agataggtgc gcgtcgcggg atcggccgag ggtgctacct cgcgcagctt
      961 cgcggcatat ttcttgtcgg gctcggacca cagcgtgacg ctggcggcgc cgtccttggc
     1021 gcgtccgacc agcgtttccg ggatcgagac cacggcttcc ttttcggcaa agcgcgccac
     1081 gcggatcgcg gtctgtccgg acgccacgac ctgtccggcg tcgatcaggg tcgcggtgac
     1141 gacaccgcgg gtatcggcga ccagcgtcgc gtaagagaga gagttcttgg tgagttccac
     1201 cgagcgctcg gcgcgattga gccgcgcacg cgcttcatcg ccggcggctt tggcctgatc
     1261 cagttgcgca tccgtggtcc agcctttgac gcgcaaatcc ttcgcgcggg tttcggcggc
     1321 ggcggcctgt gccagcacgc cggtggcggc atggaattcg gcatcggcct gctcggcctg
     1381 cagcttcaga tcgacttcgt cgagggtcgc caggggttgg ccgacatcga cggtctggcc
     1441 gacttcgacc agccgttttg ccaccttgcc ggggacccgg aaccccatgt cggtctcaat
     1501 ccggggcttg atggt
//
LOCUS       DASWGM010000780         9845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1589914,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000780 DASWGM010000000
VERSION     DASWGM010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589914"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VGA15_21510"
     CDS             <1..633
                     /locus_tag="VGA15_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269472.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9590308.1"
                     /translation="YGATEGLGFTACNSAEWLAHRSTVGKVLLGDLHILDENMQPCPV
                     GTPGTVWFKTATPFEYFNDPNKTKEARSPDGSMSTVGDVGYVDGDGFLYLTDRATFMI
                     ISGGVNIYPQECENLLITHPKIADAAVFGVPNADLGEEVKAVVQPMPGILAGRDLAEE
                     LMLFCSQSLSRQKVPRSIDFEAELPRLPTGKLYKRLLRDRYWGDKTSRIL"
     gene            complement(648..2063)
                     /locus_tag="VGA15_21515"
     CDS             complement(648..2063)
                     /locus_tag="VGA15_21515"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX9590309.1"
                     /translation="MNRAMFHRTAIAFGCAALVVGPALAEEPARLSGYNANISESSVS
                     GISSGAFMAVQFGTAWSSVIKGVGVVAGGPFWCAKADADDFINAFTLPIMTATGSCMS
                     GPLSDLSGFFAKADAKSASGDIDSLQFVRRQKVYIFHGYNDAVVARPVTDAAAAFYRH
                     YLGETNRGNLYYQTTIGAGHSLVIAQDPHKAGINDCKDNAVPFIDACGYDQAGIILQH
                     IYGALNAPNRGQLTGTTKRFDQSVYTKPDDPGMLSLGDTGYVFVPKPCEDGEACRVHI
                     ALHGCKQDTGDIGRRFIDDTGYNSWADTNHLIVLYPQTTSSSLLPLNPEACWDWWSYV
                     DHEDSYVTKSGSQIRTIKAMLDALTARAAPAPAPAVAPTSLTVIDASDTGADLAWTAQ
                     AGTTGYRVRRAGADGQFAPLGDVAGPSFADSGLTPQTAYRWRISAVANGIEDPAFIEA
                     AAITRAVPPPCENPGGCAIGK"
     gene            2262..3149
                     /locus_tag="VGA15_21520"
     CDS             2262..3149
                     /locus_tag="VGA15_21520"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX9590310.1"
                     /translation="MDVVPLGPGFAAELRGVTLKDVASDDVAYAATRTAFEEHSVLVL
                     RGQEVTDELQLAFSRRFGPPEVTKVGSQGTGSHFVILSTIGPDGKVVPSDHRQALRSK
                     ANQLWHTDSSFKRVPALTSILSARIIPARGGETEFVSTRLAFERLDAGLRNRLENSFA
                     WHDYAHSRGQISADLASPEERAALPPQCWRMVWKNPVNGRGAIYLASHAYAVEGMEAA
                     AGKKLIDELMAAATAPGTSYLHQWRGGDVVMWDNRATMHRGRPWPAHEARLMVRTTIS
                     ATEADGVATMHRPSSQAAE"
     gene            complement(3238..3483)
                     /locus_tag="VGA15_21525"
     CDS             complement(3238..3483)
                     /locus_tag="VGA15_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9590311.1"
                     /translation="MMYPRRFARVRMTGKSSKAKLIVGPKAPVVDCHVIDYSAGGACL
                     EVDPNTRLPNRFELVFGVTKKRCRVVWTAGRRLGVVF"
     gene            complement(3673..5616)
                     /locus_tag="VGA15_21530"
     CDS             complement(3673..5616)
                     /locus_tag="VGA15_21530"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9590312.1"
                     /translation="MLEAYLEATLAVRDPSTVTARRDRLVQLHKDYDERREYWIKSDL
                     EQSLKSKLTKASDTEVRRFWTIIEQEMLPALEKKAAGAAETAYAKLDPGYAAHRAIID
                     DIVKQTNDENAALETTAARRVTSFSYIVWFVSGVVVLIIALGILGIALGVIRPVVRMT
                     AVMKRLAQGDLDVEIPSAARKDEIGQMAQTVEVFKTSTIENVKLREHQRQSAEDATEA
                     KRSAMLKMADAVEHETTKSVQSISTATKGVDGAARGLTVLATTLSSDSQAVAAASEQS
                     LANAQTVSAAAEQLAASIREIGSQIERASQVTGNAVRSGHRAQETIQSLSAVVTKIAE
                     MSGNIGSIASQTNLLALNATIEAARAGEAGRGFAVVASEVKSLSHQTAQSTEEINRLV
                     AEIQAATDAAATSVGQIGQEIGEVDRVASSIAAAIQQQGAATREIARSVDQSAQSSRE
                     VSSRIANVSGGAKEVSIRAVEVQGAIANASANIAALRSILVRVVRTSSEDANRRVFPR
                     FRIDVPLAVTNSRGSRTASKFIDISEGGARIASVPDLNLQDPCTLHIEGIACINCLHR
                     PGKDRTPNAAGARRGRRNQAAIFGVASSTDVGAGGSLKQVSTNDLPPASPVRNGRRQG
                     LPSRHILARRRLAAAREHLSREM"
     gene            6224..6607
                     /locus_tag="VGA15_21535"
     CDS             6224..6607
                     /locus_tag="VGA15_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590313.1"
                     /translation="MWWFNKGERKEIWDEDIQWPIGDIEAAHKIRDICRSATDSAEKL
                     AGSADRSDNKIKHEAERYERAAKTAMKIAIKISDDLLRDSAVRQIVNLCLQANDVRTA
                     RILFRAIQAVSIREDVLNDYPVLRE"
     gene            complement(6635..7309)
                     /locus_tag="VGA15_21540"
     CDS             complement(6635..7309)
                     /locus_tag="VGA15_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX9590314.1"
                     /translation="MIPWEHIDTARVPGADVELRLMRRGTDFSMMLGPNELMSSRLSG
                     SEAALATLACRRIEAVKRPHLLIGGLGMGFTLRAALAVLGTEARIVVAELVPAVIAWA
                     RGPMADIFGDSLDDPRASIRSADVIEVIESHASAFDAILLDVDNGPEGLIRKANDALY
                     DLEGLKTIRRALHLGGVLAVWSSGPNASFSKRLRAAGFDVNEVAVRANAKRSGARHVI
                     WFATKS"
     gene            7592..9139
                     /locus_tag="VGA15_21545"
     CDS             7592..9139
                     /locus_tag="VGA15_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9590315.1"
                     /translation="MLKKIVFGLVLTCSAGLALAQEPKMGGVINAVIQPEPPGLMLAM
                     IQNGPTQMVSGNIFEGLLRYSPKLEPLPGLAESWTVSEDAKVYTFKLKPGVTWHDGKP
                     FTSADVLFSLEFLKQFHARARNNLLQVEKVEAPDDLTVVFTLKQPFGPFLGIFEVGSL
                     PMVPKHLYEGTDYKTNPYNNAPIGTGPFMFKEWQKGSFIRLVKNPNYHIKGKPYIDEI
                     YWQVIPDAAARSVAFETGKVDVLPGGSVENFDVPRLSKLKDVCVTGAGWEFFSPLAWM
                     WLNNRAGPTSNKKFRQAVMYAIDRNFAKDVIWNGLGKVATGPSASTIKFYTDDVPKYD
                     FNPTKAKALLKEAGYNGEKVRLMPLPYGETWLRWGEAVKQNLQDVGINIETVATDVPG
                     SNQKIGDWDYDMAFSYLYQYGDPALGVGRNYVSSAIAKGAVFNNVEGYSNPEIDKLFA
                     DGAVATPDSRRQEIYEKAQKILVEDVPVAWMLELQFPTITRCKIKDLVTTAIGVNDGF
                     RDAWIDK"
     gene            complement(9247..9591)
                     /locus_tag="VGA15_21550"
     CDS             complement(9247..9591)
                     /locus_tag="VGA15_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX9590316.1"
                     /translation="MDRFKARARNLRAPQTSAEAKLWQALRNRRMARWKFRRQHSIDR
                     YVVDFVTPDGKLIVEVDGVTHAEPSELTRDKARSDVLEACGFLVVRVSNTDVYDNLEG
                     VLEMVESTLGLD"
     gene            9711..>9845
                     /locus_tag="VGA15_21555"
     CDS             9711..>9845
                     /locus_tag="VGA15_21555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590317.1"
                     /translation="MLSFVAQRIVKGAIVLLAIIVLNFFLIRLAPGDPAVVMAGEAGA
                     G"
BASE COUNT         1962 a         2999 c         2938 g         1946 t
ORIGIN      
        1 tacggcgcca ccgaaggcct gggctttacc gcctgcaaca gcgcggagtg gctggcgcac
       61 cggagcaccg tcggcaaggt cctgctcggc gacctgcaca ttctcgatga gaatatgcag
      121 ccctgcccgg tcggtacgcc cggcacggtc tggttcaaga ccgccacgcc gttcgaatat
      181 ttcaacgatc cgaacaagac caaagaggcc cgctcgcccg atggcagcat gagcacggtc
      241 ggcgacgtcg gctatgtcga cggcgacggc tttctttatc tcaccgatcg cgcgaccttc
      301 atgatcatct ccgggggcgt caacatctac ccgcaggaat gcgagaacct cttgatcacc
      361 catcccaaga ttgcggacgc cgccgtgttc ggggttccca atgccgacct cggcgaggaa
      421 gtcaaagcgg tggtgcagcc gatgccgggg attttggcgg ggcgggatct ggcggaagaa
      481 ctgatgttgt tttgcagcca atccctgtcg cgccaaaaag tgccgcgctc gatcgatttc
      541 gaggcggagc tgccgcggct gccgaccgga aagctctaca agcgcctgtt gcgcgaccgc
      601 tactggggcg acaagacgtc acggatattg tgaccgttga gtttcattca cttgccgatc
      661 gcgcaaccgc ccggattttc gcaaggcggg ggaacagccc gcgtgatggc ggcggcctcg
      721 atgaaagccg ggtcttcaat tccgttcgca accgccgaga tgcgccagcg ataggcggtt
      781 tgcggcgtca ggccggaatc ggcgaaactg ggacctgcca catctcccaa cggcgcaaac
      841 tgaccgtcgg cgcctgcgcg ccggacgcgg taacccgtcg ttcccgcttg cgcggtccac
      901 gccagatccg cgccggtgtc ggacgcgtcg atcaccgtga gcgaggttgg cgccacagcg
      961 ggtgccggcg caggcgctgc gcgcgcggtc aacgcgtcga gcatggcctt gatcgtcctg
     1021 atctgcgatc cggacttcgt gacatagctg tcctcgtgat cgacgtaact ccaccagtcc
     1081 cagcatgctt ccggattgag cggcagcaac gagctcgacg tggtctgcgg ataaagcacg
     1141 atcagatgat tggtgtctgc ccaggaattg tacccggtat catcgatgaa acgccgaccg
     1201 atatcgccgg tatcctgctt gcacccatga agcgcgatat ggacgcggca cgcctcgcca
     1261 tcctcgcatg gcttgggaac gaacacgtag ccggtgtcgc ccaggcttag cataccgggg
     1321 tcatcgggct tggtataaac agattggtcg aagcgcttcg tcgttccggt caattgtccc
     1381 cggttggggg cgttcaacgc gccgtaaatg tgctggagga tgattccggc ctggtcatag
     1441 ccacacgcgt cgataaacgg gacagcgttg tccttgcagt cgttgatgcc ggccttgtgc
     1501 ggatcctgcg caatgaccag cgaatgaccg gcaccgatcg tcgtctggta gtaaaggttg
     1561 ccgcggttgg tctcgcccag ataatgccga tagaaggccg cggccgcatc ggtgaccggc
     1621 cttgccacca cggcatcgtt gtagccgtgg aagatatata ctttttgccg gcgcacgaac
     1681 tgcagggaat cgatatcgcc ggaagccgat ttggcgtcgg ccttcgcgaa aaagccgctc
     1741 aggtcggaaa gcggcccgga catgcatgag cccgtcgcgg tcataatcgg gagcgtgaag
     1801 gcgttgataa agtcgtcggc gtccgccttg gcgcaccaga acgggccgcc ggcaacgacg
     1861 ccgacgccct tgatcaccga agaccaggcg gtgccgaact ggacggccat gaacgctccg
     1921 gacgagatgc cggataccga gctttcgctg atgttggcgt tgtagccgga tagccgcgcc
     1981 ggctcttcgg cgagtgccgg gcccaccacc aacgccgcgc agccaaaagc gatcgcagta
     2041 cgatgaaaca ttgctcggtt catgagacgc tccctgttga actgtgtcag cccgttagaa
     2101 gcagacgtca tcggcgcgct gatttcgtgc ccgccgtgaa tgtttgccga acaccacggc
     2161 tcggacgccg accgtcaggc ggtaacgctt cagttcattg ccggccggga aattttaccg
     2221 taaagttacg caataacaat aaaaacaggg aggacttgcc gatggacgtt gtgccgttgg
     2281 gtccgggatt cgccgccgaa ctgcgcgggg tgacgctcaa ggatgtcgcc tcggacgatg
     2341 tcgcatatgc cgcaacacgc accgcttttg aggaacattc cgttctggta ttgcgcggcc
     2401 aggaggtgac cgacgagctt caactggcgt tttcgcgccg cttcggccca ccggaggtga
     2461 ccaaggtcgg ctcgcagggt acggggtcgc attttgtgat cctgagcacc atcggccccg
     2521 acggcaaggt ggtgccatca gatcaccggc aggcgttgcg cagcaaggcc aaccagctct
     2581 ggcataccga ctcgtcgttc aagcgtgtgc cggcgctcac ctcgatcctt tcggcgcgga
     2641 tcatcccggc gcgcggcggc gagaccgaat tcgtctcgac ccgacttgcc ttcgagcggc
     2701 tcgacgcggg cctgcgcaac aggctcgaga attcctttgc ctggcacgac tatgcgcatt
     2761 cgcgcggcca gatatcggcc gaccttgcga gccccgagga gcgcgcggcg ctgccgccgc
     2821 aatgctggcg catggtctgg aagaatcccg taaacggtcg cggcgcaatc tacctcgcct
     2881 cgcatgccta tgcggtcgaa ggcatggagg cggcggcggg gaagaagctg atcgacgaat
     2941 tgatggcggc cgcgaccgcg cccggcacga gctacctgca ccaatggcgc ggaggtgacg
     3001 tcgtgatgtg ggacaaccgc gcgaccatgc atcgcggacg gccgtggccg gcgcacgagg
     3061 cgcggctgat ggttcgtacc acgatctccg cgaccgaggc cgatggcgtt gcgaccatgc
     3121 accggccgtc atcgcaggcc gcggaatagc agtgaaaaaa gcggacgctc gccgctgcga
     3181 gcctcaggtc cgatttgcat cgaattcgga agccgttcag attcaacggc gggtttgtta
     3241 gaagaccacc ccaagacgtc gaccggcagt ccaaacaacc cggcagcgtt tcttggtgac
     3301 gccgaacacc aattcgaatc gatttggcag ccgggtattc ggatctacct caaggcatgc
     3361 gccgccagcg gaatagtcaa taacatggca atcgacaacg ggagctttgg ggccaacgat
     3421 gagtttggcc ttcgagctct tgcctgtcat tcgaacgcga gcaaaccgtc gcggatacat
     3481 cactggtccc tatctatgac ctgaaaccca ttggataggt tcacagacta tcctgaaaat
     3541 ttcgtttcag ttttccatac atattcccgc gtcgcgccga gcgcgcctga acagatatgg
     3601 tcgcccgccg cactatgata ccgctactca gtgcgatcgc agatggactg acatccggag
     3661 gtttcagtcg gctcacatct ctcgcgagag atgctctcgt gccgcagcca atcgccgtcg
     3721 cgccagaatg tgccgcgacg gcaaaccctg cctacggccg ttgcggaccg gcgacgctgg
     3781 cggcaagtcg ttcgtactca cttgcttcag gctgccacca gccccgacat ctgtcgatga
     3841 agccacgcca aatattgctg cctggtttcg ccttccgcgg cgagctccgg cagcgtttgg
     3901 tgttcggtct tttcccggac gatgaaggca attgatgcaa gcaataccct cgatatgaag
     3961 ggtgcaggga tcctgaaggt tcagatccgg aacgcttgca atgcgcgctc cgccctccga
     4021 gatgtcgatg aatttggacg ctgttcgcga tccccgtgaa ttggtaacag ccagcggcac
     4081 gtctatccga aatcgaggga acactcgtcg attggcatct tctgacgatg tccgtacaac
     4141 ccggaccagg atcgagcgca acgccgcgat gttcgccgat gcattggcaa tcgctccttg
     4201 tacttcaacc gcgcgaatgc tcacttcttt ggcgccgccg ctgacgtttg caattcgcga
     4261 gctgacctcc cggctggatt gcgcggattg atccacgctg cgcgcgatct ctcgcgtcgc
     4321 ggcaccttgc tgttggatag ccgccgcgat cgaactggcg acacgatcga cctctccgat
     4381 ttcctgtccg atttgtccga ccgatgtggc cgccgcatcc gtcgctgcct gtatttcagc
     4441 taccaggcga ttgatctcct cggtggattg cgcggtctga tgggaaagcg acttcacttc
     4501 cgacgccacg acggcgaaac ctcttccagc ctctcccgcg cgggcagcct cgatggtcgc
     4561 attcagcgcc agcagatttg tctggctggc aatgctgccg atgttaccgc tcatttcggc
     4621 aatcttggtc acgacggccg agagagattg aattgtttcc tgagcgcgat ggccgctgcg
     4681 aaccgcattg cccgtgacct gactggcccg ttcgatttga gatccaatct cccggatcga
     4741 tgcggccaat tgttcggctg cggccgaaac cgtctgggcg ttggccagtg attgttcaga
     4801 tgccgcggcg acagcctgcg aatcagacga caaggttgtc gccaacaccg tcaatccccg
     4861 cgcggcgccg tccacgccct tggtggcggt agaaatcgac tggaccgatt tggttgtctc
     4921 gtgttcgacg gcgtccgcca ttttaagcat ggcgctgcgc ttggcctcgg tggcgtcttc
     4981 agccgattgg cgctggtgct cgcgcaactt cacgttctcg atcgtgctgg ttttgaagac
     5041 ctccacggtc tgtgccattt gaccgatttc gtctttccgc gcggcggaag gtatctcgac
     5101 atccagatcg ccctgagcca accgcttcat gacagccgtc atgcggacga caggccgtat
     5161 gacccccaac gcgatgccaa ggatacccag ggcgataatg agcacaacga ccccggagac
     5221 gaaccacaca atatacgaaa acgatgtcac gcgccgggcg gcggtggttt ccaatgctgc
     5281 gttttcatcg ttggtctgtt ttacaatgtc atcgatgatg gcgcggtggg cggcgtatcc
     5341 cgggtccagc ttcgcgtagg cggtttcggc ggcgccggca gcttttttct caagcgcggg
     5401 caacatttcc tgttcgatga tggtccagaa ccgccgcacc tcggtatcgg aggcctttgt
     5461 tagctttgat ttcaacgatt gttcgaggtc ggatttgatc cagtactcgc ggcgttcgtc
     5521 gtaatccttg tgcagttgaa ccaaccggtc tcgccgggct gtaaccgtcg atggatcacg
     5581 caccgcaagg gttgcctcga gataggcttc gagcacatat tccggcggcg gcaggatatc
     5641 ggcgaccaga tcgtttccga gctttatctg tgtgtatagg ggaccgccga tgcgcaattg
     5701 ctgcagcgcg tacccgctcg taaacaacac ggcaaaaagc ccgacggccg taacgaggcc
     5761 aaagatggta atggcgacgg agattgagat ccgcgatttc atgcttatcc gataccccaa
     5821 tccgccaggt cagcagtgaa actttcaccg cgccgacgat tccgcttcga cggtaggaca
     5881 ccggaggctg caagctcatt aatttgagca accgtacaac tacgaggtgt acagcccgga
     5941 cgccgccgga gatccggtcc ggcatcgacg ccgggcgact tcgccgtctt ggcgctgatc
     6001 ctcggctcca acctgaccgc ggcctggacc ggaagtccgc tcggcttgtc gcccccgagg
     6061 acgggatcgc cataagggcg ccgtttccga cccggttgac ccccgcagcg cagcctcgtt
     6121 cgaacggacg atggcgcacg gcgcgcaact ggtgctccgg tggaatgaat attgaaatcg
     6181 taccgcggcg atctatgctt gcagatattc aagggcgcgg gcgatgtggt ggttcaacaa
     6241 gggcgagcgt aaggaaatct gggacgaaga tatccagtgg ccgatcggcg atatcgaagc
     6301 ggcgcataag atacgcgaca tctgccgttc cgctaccgac agcgctgaaa aactagccgg
     6361 ctcagctgac cgctcagaca ataaaatcaa acacgaggcc gaaagatacg aacgcgccgc
     6421 caagaccgcc atgaaaatcg ccatcaagat ttccgatgac ctgttgcgcg attccgccgt
     6481 gcgccagatc gtcaatttgt gtttgcaggc caacgacgtg aggacagcgc ggatactgtt
     6541 tcgcgcgatt caggccgtgt cgatcagaga agacgtgctg aatgactatc cggtactgcg
     6601 ggagtgaatt tccggcgagc cggaaacgcg gacctcaaga cttcgtcgca aaccagatca
     6661 catggcgcgc gccgctgcgc tttgcgttgg cgcgcacggc cacctcgttc acgtcgaagc
     6721 cggcggcgcg aagccgtttg gaaaacgaag cgttaggtcc cgacgaccaa acggcgagga
     6781 cgccgcccag gtgcaaggcc cggcggatcg ttttcagccc ttccagatca tagagcgcat
     6841 cattggcttt gcggatcagg ccctccgggc cgttatcgac atcgagcagg atcgcatcga
     6901 acgccgaggc atgggattct atcacctcaa tgacgtcggc gctgcgaatg cttgcgcggg
     6961 gatcgtcaag gctatcgccg aaaatgtcgg ccatgggacc tcgtgcccac gcgatcacgg
     7021 cgggcacaag ctcggccacc acgatccgcg cttcggttcc caatacggca agcgcggcgc
     7081 gcagggtgaa tcccatgccc aatccaccga tcaacagatg cgggcgcttg accgcttcga
     7141 ttcttcgaca ggcgagtgtg gcaagcgcgg cctcagatcc actgaggcga ctgctcatca
     7201 gctcgttggg gccgagcatc atggagaaat cggtacccct tcgcatcaga cgaagttcga
     7261 cgtctgcgcc aggcacgcgt gcggtatcga tatgttccca cggaatcatg cgaagcttct
     7321 acgctgtttt gacgccatcg ggcgaggtct ggcacacacg accaccacgt cgcactctgt
     7381 ttttgctggg cgtcgcaggc ataggcgtcg agccgcgaat tgtccaaatt ttagtcgccg
     7441 tttgtcgaca gtttcatctt gcggcctgga ttggcgccat gtgagcctct tcccgatatg
     7501 gggcttttca acctacccaa agcctgaatt ctcgtgcttc aacagggctt gccgcgcaga
     7561 gtccgcggat cgagccatca aaaggtaagt catgctgaag aaaatcgtgt tcggcctcgt
     7621 tttgacctgc agcgcaggcc ttgcgctcgc gcaggagccg aagatgggag gtgtcatcaa
     7681 cgccgtgatc cagcccgagc cgcccggcct gatgttggcg atgatccaga acggcccgac
     7741 ccagatggta tcaggcaata tctttgaagg tctgctgcgg tacagtccga aactggagcc
     7801 gctgccgggc ctcgccgaaa gctggacggt cagcgaggac gccaaggtct acaccttcaa
     7861 gctgaagccg ggcgtgacct ggcatgacgg caagccattc acgtcagccg acgttctgtt
     7921 ctcgctcgaa tttctcaagc agtttcatgc ccgcgcacgc aacaatctgc tgcaggtcga
     7981 gaaggtcgag gcgccggatg atttgacggt tgtattcacg ctcaagcagc cgtttgggcc
     8041 attcctcggc atcttcgagg tcggctcact gccgatggtc ccgaagcatt tgtacgaagg
     8101 caccgactac aaaaccaatc cttacaataa cgcgccgatc ggtaccggcc cgttcatgtt
     8161 caaggaatgg cagaagggct cattcattcg tctcgtgaag aatcccaact accacatcaa
     8221 gggcaagccc tacatcgatg aaatttactg gcaggtgatt ccggatgccg cggctcgctc
     8281 ggtcgcgttc gagaccggca aggtcgacgt gctgcccggc ggatcggtgg agaatttcga
     8341 tgtcccgcgc ctcagcaaat tgaaggacgt ctgcgtcacc ggcgccggct gggaattttt
     8401 cagcccgctg gcctggatgt ggctcaacaa ccgcgccggc ccgacgtcga acaagaaatt
     8461 ccgtcaagcc gtgatgtacg cgatcgaccg caacttcgcc aaggacgtga tctggaatgg
     8521 gctcggcaag gtcgccaccg gcccgtcggc ttcgaccatc aagttttaca cggatgacgt
     8581 tccgaaatac gacttcaacc caaccaaggc caaggcgcta ctgaaggaag ccggctacaa
     8641 cggcgagaag gtgcgtctga tgccgctgcc ttacggcgag acctggctgc gctggggcga
     8701 agccgtcaag cagaacctgc aggacgtcgg catcaatatc gagaccgtcg ccaccgacgt
     8761 gcccggttcg aaccagaaga tcggcgactg ggactacgat atggccttct cttatcttta
     8821 ccagtacggg gatcctgcgc tcggcgtcgg ccgcaactac gtctccagcg cgatcgccaa
     8881 aggtgcggtg ttcaacaacg tcgagggcta ttccaacccg gagatcgaca aattgtttgc
     8941 cgacggcgcg gtcgcgacgc cggattccag gcgccaggaa atctacgaaa aggcgcagaa
     9001 aatcctggtg gaggacgtgc cggtggcatg gatgctcgaa ctgcaatttc cgaccatcac
     9061 ccgttgcaag atcaaggacc ttgttacgac cgcgatcggc gtcaatgacg gcttccgcga
     9121 cgcctggatt gacaagtgag aacattttcc ggaggaaaaa gtgagcaacg aacgctgccg
     9181 ctttcttcac ctctccccgc cggggagagg tcggctgcgt agcagccggg tgaggggcct
     9241 ttccattcaa tcgagcccga gtgtgctttc aaccatttcg aggacaccct caagattgtc
     9301 atacacgtca gtattcgaga cgcggacaac gaggaagcca caggcctcca atacgtcgga
     9361 ccgcgcttta tctctcgtca gctccgatgg ttcagcgtgc gttacgccgt caacttcgac
     9421 gatgagtttt ccgtcaggcg ttacgaagtc gacgacgtac cgatcaatgg aatgctggcg
     9481 gcgaaacttc caccgcgcca tccgtcgatt acgcagcgct tgccaaagct ttgcctcggc
     9541 actcgtttgc ggcgcacgaa ggtttcgcgc tctagcctta aacctgtcca tccccctcac
     9601 ccggctgctg cgcagccgac ctctccccgg cggggagagg tatagcacag cgaacgccgc
     9661 aacgtgaccg cccgcttcga cgcacgcttc acggaagtgg tggacgactg atgctgtcat
     9721 tcgttgccca gcgcatcgtc aagggcgcga tcgttctgct tgcgatcatc gtcttgaact
     9781 tcttcctgat ccggcttgct cccggcgatc cggccgtcgt catggccggc gaggccggcg
     9841 ccggc
//
LOCUS       DASWGM010000781         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1045802,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000781 DASWGM010000000
VERSION     DASWGM010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045802"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VGA15_21560"
     CDS             complement(<1..363)
                     /locus_tag="VGA15_21560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX9590318.1"
                     /translation="MTDMTPASSSPASKPKPRPRPQVMKLTEAAAHRIAELTRRADSE
                     IVGLRVGIKNGGCAGQSYTVEYAHEVRATDEVVEDKGVKILVDPKAVLFLLGTEMDYK
                     VDKMSAQFIFNNPNQTGAC"
     gene            complement(435..821)
                     /locus_tag="VGA15_21565"
     CDS             complement(435..821)
                     /locus_tag="VGA15_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEX9590319.1"
                     /translation="MTDTVEAKDLEVKTGNMQTNSALPPEETERLGGEIVAALKTVFD
                     PEIPADIYELGLIYKVDIKDDRTVDVEMTLTTPNCPAAGELPQMVENAVASVPGVGVV
                     NVNLVWDPAWTPDRMTDEARLVLNMW"
     gene            complement(818..2065)
                     /locus_tag="VGA15_21570"
     CDS             complement(818..2065)
                     /locus_tag="VGA15_21570"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX9590320.1"
                     /translation="MTMHPAVLNGSYDVARVREDFPALALKVYGKPLVYLDNAASAQK
                     PNAVLDRMTEAYKSEYANVHRGLHYLANAATEAYEGGRAKVAKFLNAGRNEEIVFTRN
                     ATEAINLVASSWGEPNIKAGDEIVLSIMEHHSNIVPWHFLRERHGAVIKWVDVDDEGN
                     FLIDAFEKALTPRTKLVAITQMSNALGTIVPVKDVVKLAHARGIPVLIDGSQAAVHLA
                     IDVQDIDCDFYVFTGHKLYGPTGIGVLYAKHEHLVAMRPYNGGGEMIREVARDWVTYG
                     DPPHKFEAGTPAIVESIGLGAAIDYVNSIGKERIAAHEHDLLTYAQERLREINSLRLI
                     GTAKTKGPVISFEMKGAHPHDVATVIDRQGIAVRAGTHCVMPLLERFNVTATCRASFG
                     MYNTREEVDHLAQALIKARELFS"
BASE COUNT          369 a          658 c          629 g          447 t
ORIGIN      
        1 gcaggcgccg gtctggttcg ggttgttgaa gatgaactgc gccgacatct tgtcgacctt
       61 gtagtccatc tcggtgccga gcagaaacag cacggccttg ggatcgacca gaatcttcac
      121 gcccttgtcc tcgaccacct cgtccgtggc gcgcacctcg tgggcgtatt caaccgtgta
      181 ggattgcccg gcgcagccgc cgttcttgat cccgacccgc aggcccacga tctcggaatc
      241 ggcgcgtcgg gtcagctcgg cgatccggtg cgctgccgct tcggtcagct tcatcacctg
      301 cggacgcgga cgcggcttcg gcttggatgc aggacttgat gaggcaggtg tcatgtcagt
      361 catgtggtca atccaaaagg caaatcaatg ccggctatct tcccgtgatc gagttaagtc
      421 ccaaacaatc aagctcacca catattgagg acaaggcgcg cctcgtcggt cattctatcc
      481 ggcgtccatg ccgggtccca aaccaggttg acattgacga cgccgacgcc ggggacactg
      541 gcgaccgcgt tctcgaccat ctgcggcaac tctccggccg ccgggcagtt tggcgtcgtc
      601 agcgtcatct cgacgtccac ggtgcggtcg tccttgatgt cgaccttgta gatcagaccg
      661 agttcgtaga tatcggccgg aatttccgga tcgaacacag tttttagcgc ggcgacgatt
      721 tcgccgccga gccgctcagt ctcctccggc ggcaacgccg aattggtctg catgttgccg
      781 gttttgactt cgaggtcctt ggcttcgacg gtgtcggtca tgaaaacaat tcccgcgcct
      841 tgatcagcgc ctgtgccaga tggtcgactt cttcacgggt attatacatc ccgaacgagg
      901 cgcggcacgt cgccgtgaca ttgaaccgct ctaaaagcgg catcacgcaa tgggtgccgg
      961 cgcgtaccgc gatcccctgc cggtcgatca cggtggcgac atcgtgggga tgcgcgccct
     1021 tcatctcgaa cgagatcacc ggtcccttgg tcttcgcggt gccgatcagg cgcagcgaat
     1081 tgatttcgcg caggcgctcc tgggcatagg tcaaaaggtc gtgctcatgg gcggcgatcc
     1141 gttccttgcc gatcgaattg acgtagtcga tcgcagcccc cagcccgatc gattccacga
     1201 tcgccggcgt gcctgcctcg aatttgtgcg gcggatcgcc ataggtgacc cagtctcttg
     1261 cgacctcgcg gatcatctcg ccgccgccat tatagggccg catcgccacc aggtgttcgt
     1321 gcttggcgta gagcacgccg atgccggtcg ggccatagag cttgtggccg gtgaagacgt
     1381 agaaatcgca atcgatgtcc tgcacgtcga tcgccagatg caccgcggcc tggctgccgt
     1441 cgatcagcac cgggatgccg cgggcatggg caagcttcac gacatccttg accggcacga
     1501 tggtgccgag cgcattcgac atctgggtga tcgcgaccag tttggtgcgc ggcgtcagcg
     1561 ccttttcgaa cgcgtcgatc aggaaattgc cctcgtcatc gacgtcaacc cacttgatga
     1621 cggcgccgtg acgttccctg aggaaatgcc agggcacgat gttggaatgg tgctccatga
     1681 tcgaaagcac gatctcgtcg ccagccttga tgttgggctc gccccatgac gacgccacga
     1741 ggttgatcgc ctcggtggcg ttgcgggtga acacgatctc ttcgttacgc ccggcgttga
     1801 ggaattttgc gaccttggcg cggccgccct cataggcctc cgtcgccgca ttggcgagat
     1861 aatgcagccc gcgatgcacg tttgcgtatt cgcttttgta ggcttccgtc atgcggtcga
     1921 gcaccgcatt gggcttttgc gccgacgccg cgttgtcgag atagaccagc ggcttgccat
     1981 agacttttaa cgccagcgcc ggaaagtctt cccgcacccg ggcgacatca tatgaaccgt
     2041 tcaggactgc cggatgcatg gtcaacctct tgccgccagc cagcgctgcg ccgccgagat
     2101 cgc
//
LOCUS       DASWGM010000782         5190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1454207,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000782 DASWGM010000000
VERSION     DASWGM010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454207"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..277
                     /locus_tag="VGA15_21575"
     CDS             146..277
                     /locus_tag="VGA15_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590321.1"
                     /translation="MRALIRQMSTENSLSGAPRIHGETSSGLIALLARASSVGEVIE"
     gene            313..840
                     /locus_tag="VGA15_21580"
     CDS             313..840
                     /locus_tag="VGA15_21580"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HEX9590322.1"
                     /translation="MSGAIFETGFEATTTAVAPAVPRVFAFSMQAEQSIVVDPKSHGG
                     INSPHGLILFDGVCVLCSRGCRFVSKRDRRGYFRFVPIQLAEGRPLAEHLGIDPDRPD
                     SFAFLASGQAYIKSEAVLRIARELPRWQWTWIFQFIPRVIRDATYDLVARNRYRWFGR
                     RDACILPNSDRSWPS"
     gene            complement(928..1164)
                     /locus_tag="VGA15_21585"
     CDS             complement(928..1164)
                     /locus_tag="VGA15_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX9590323.1"
                     /translation="MESYPKFHNEVGVRIVRIGCREFKCIGDKPPQDHPHIYLKMGDA
                     CEIVCPYCSTLFRFDPSLGAHEADPADCVYGDRD"
     gene            1490..2188
                     /locus_tag="VGA15_21590"
     CDS             1490..2188
                     /locus_tag="VGA15_21590"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9590324.1"
                     /translation="MAKAVGKTHFNFSINAVERETLNDRVYRELRTMIMSGGFEPGAE
                     LTLRALAQALKVSLMPVRDAIGRLVAERALEMLPNRKVIVPEITIDGFLEIRRVRILL
                     EGEAAALACQHITKDGIDALKRYHVRIKSLGSNKPRQFFSFNQKFHFVIYEAARSPLL
                     LSIIESLWLQIGPVFNHIPHNFAAEGARGHDRIIAALEARDAKAARAALENDLMKGSD
                     RIIATLIGAKQPAT"
     gene            2594..4297
                     /locus_tag="VGA15_21595"
     CDS             2594..4297
                     /locus_tag="VGA15_21595"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9590325.1"
                     /translation="MAKLSAGRAVVEVLKAEGVKFVFGMPGGHTIGIYDALYGQQEIT
                     HVLVRHEQHAASMAAGYAQLTGEPGICCVTAGPGATNLVTSIAEAFVGALPIVIIAGR
                     GATRNAHRGASQEIDQVRIFGPITKWAIRVDRPDLIVESLRQAFNVARSGKPGPVLVD
                     IPQDVLASDIEFSNYKPVGKPARSCGDAVQIKAAAAALRSAKRPLIVSGGGAIASDAA
                     GEVLRLAEALAIPVATTLAGRGILPDDHVLAAGGLGHHRQEITKRLLPNADVVLGIGT
                     RFEQQETNWKPDYLPAPAATYIQIDVDPAEIGRSVVATIGIVGDAKLVLQDLLAELDQ
                     SCVASRWNDADTHTGADDLARQRAALESEISEMVASDSRPLHPIRVIRKIRDAFPRHA
                     TVAIDVGVLAQGMGGAFPYFKIYQPRSTIVPSSFYGMGFSASAFPVARLVYPDRPAVC
                     LVGDGSFQMVMNILPVAAELKLGVTWCILNDGALGSIRDIQAQAFSERYIGTEFALDL
                     DFAKLAQASGCYGERVTEPAEIENALKRAIDANEGGRPAVLDFFVAKERLKGSVEFFK
                     R"
     gene            4294..4848
                     /locus_tag="VGA15_21600"
     CDS             4294..4848
                     /locus_tag="VGA15_21600"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9590326.1"
                     /translation="MKFLSFGQPVGGIVQAAYTVENIDRAMSDFTQRLNVGPWFVTGP
                     FTPSKGYYRGQPTDMSLTLAVAFAGHMMIELIEQHDDKPSVYQETIKIKGHGFHHWAI
                     CSKSFDQDVARYEAAGYPIAFSDLSPRGVRIVYMDTTRDLPGMLEIIEATDALEAIYQ
                     SYFDAAQGWDGRDPVRRWSLKTGA"
     gene            4850..>5190
                     /locus_tag="VGA15_21605"
     CDS             4850..>5190
                     /locus_tag="VGA15_21605"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590327.1"
                     /translation="MKTQDLFDVAGLSVLITGGAKGLGRAFAEALLDNGARVTLLDCD
                     GECLDRAVIELRDRGTAVHGDVVDVTDRAAMMKAVDRTVDRLGRLDVVFANAGIDAGP
                     GFLSMSGERDP"
BASE COUNT          979 a         1524 c         1637 g         1050 t
ORIGIN      
        1 ttcatatcgc tggttcccgt cgatccttcc ggttctcatg attatgcgac ccgaaacgct
       61 ggtacgttgg tatcgggccg gctttcgccg cgattggcgt tggaaatcgc gtcgacgggg
      121 agggcgaccg ccgatcgagg cggaattgcg cgcgctgatc cggcagatga gcacggagaa
      181 ttcgctttcg ggtgcgccgc gcatccacgg cgaaacaagc tcgggtttga tcgctttgtt
      241 ggcgcgggcg agcagcgtcg gcgaggtgat cgaatgaggc ggaggttgcc gcccagtgct
      301 cggccatcag tgatgtcggg agccattttc gagacaggct ttgaagcgac aaccaccgct
      361 gttgcgccgg ccgtcccgcg ggtgtttgct ttttccatgc aggcggagca gtcaatcgtc
      421 gttgatccaa aaagtcatgg cggcatcaac tcgccgcacg gcctgatcct gttcgacggc
      481 gtatgcgtcc tatgctcgcg cggctgccgc ttcgtcagca aacgtgatcg ccgcggctat
      541 tttcgcttcg ttccgatcca gttggccgag ggacgtccgc tcgctgagca tctcggcatc
      601 gatcctgatc gcccggattc ctttgccttc ttggcgagcg gccaggccta tataaaatcc
      661 gaagccgtac tgcgcattgc gcgcgagctt ccgcgctggc agtggacatg gatcttccaa
      721 ttcatcccgc gggtgatccg cgacgcgacc tatgacttgg tcgcgcgcaa ccgctatcgc
      781 tggttcggcc gtcgtgacgc ttgcatcctg ccgaattcgg atcgttcgtg gccatcgtga
      841 accgccccga aactgcggga cgatggcgcc tgacgctggt tggctgcggc cgccatgacg
      901 aaggaagcag cgttactgca attcaactca gtccctgtca ccgtaaacac aatctgccgg
      961 atcagcttcg tgtgcgccca agctcggatc gaagcggaat agcgtagagc aataggggca
     1021 gacgatctca caggcatcgc ccatcttgag gtagatgtgt gggtgatctt gcggcggttt
     1081 atccccaatg cacttgaact cgcggcagcc aatgcgcacg atccgcaccc cgacttcatt
     1141 atgaaacttc ggataacttt ccatctgcag cgcgcggcgt agcgcccgat cgatgatgca
     1201 aagtgccatg catggatcgt cgaacgaggg ctcgatcagc acccggtgag cggcgcaacc
     1261 gccggaatcg aggcggagaa cgccggttcc gcgtcgggac tgttcaacat gatgcgcaac
     1321 ctcggcggcg cgatgggcat cgccgcgctg cagacgctgg gcgagcagtg gaggaatggt
     1381 gaggtcgcct tctgcaccga ggttgtccgg caacgtcctt caataggcgc cggatattcc
     1441 caatgcgctt aattgtctgt atggatgacc gtccattcgg aaagatttaa tggccaaagc
     1501 agtcggcaag acgcatttca atttctccat caacgccgtt gagcgcgaaa ccctcaatga
     1561 tcgcgtatat cgagagctgc ggacaatgat catgtccgga ggattcgaac ctggtgcgga
     1621 actcactctt cgcgcgttgg ctcaggctct gaaggtgagt ctgatgccgg tccgggacgc
     1681 gatcggccgg ctggtggctg agcgtgcgct ggaaatgctg cccaaccgca aggtgatcgt
     1741 ccccgagata acaatcgacg gctttctgga aatccgaagg gttcgaattc tgctcgaggg
     1801 cgaagccgcg gcgctcgcgt gtcagcacat tacgaaggac gggatcgacg cgctgaaacg
     1861 gtaccacgtc aggatcaagt cgctcggctc gaataagccg cggcagttct tctcgttcaa
     1921 tcagaaattc cacttcgtga tttatgaagc ggccagaagt ccgctgctgc tttcgatcat
     1981 cgagtcgctg tggctgcaga tcggcccggt tttcaatcat attccgcaca actttgcagc
     2041 ggagggggcg cgtgggcacg atcggatcat cgcggcgctt gaggcgcgcg atgccaaggc
     2101 tgcgcgggca gcgttggaga acgatttgat gaagggcagc gatcgcatta tcgctactct
     2161 gatcggcgcg aagcagcccg ctacctgatt tttcgcgcaa cgtctcccgg caattcgccg
     2221 tcgatcccct catggccgtc cggctgcgcc cggaatccag caagcctagg cgtttccagt
     2281 tctcggcgct tgtgctgaga tatgtgattt gttatcacaa atttcggcag ccgggcgacg
     2341 acgtcccgcg cgccggttac agcgcggggt ctggggagga aatgggcata tggaagcggt
     2401 tagggctgct tgtcggtcgg acgatggttg cgtccaccgg cccgaacgcg gccgacgcgc
     2461 acacggccgg caacagcaag cctgcaatca tgctttgaat tgaaaccagg ccaccaggcc
     2521 ctcgcgtgcg agccttcaat tcgacaaaac aacaacagct ccggagcccg ggcgccgaaa
     2581 tggatgcagc taaatggcga aacttagtgc gggtcgcgca gtcgtcgaag tcctcaaggc
     2641 tgagggggtc aaatttgtct tcggcatgcc cggtggccat accattggca tctatgatgc
     2701 cctttacggg cagcaggaaa ttacccacgt gctggtccgc catgaacagc acgcggcgag
     2761 catggcggcc ggctatgcgc aactgaccgg tgagcccggt atctgctgcg tcacggctgg
     2821 ccccggcgcg accaatctcg tgaccagcat tgccgaggca ttcgtcggcg cgctgcccat
     2881 cgtgatcatc gcggggcggg gcgcaacgcg caatgcgcat cgcggtgcca gccaggagat
     2941 cgatcaggtc cggattttcg gcccgatcac aaagtgggcg atccgggtcg atcggcccga
     3001 tctgatcgtc gagagccttc ggcaggcctt caacgtcgcc cgctcgggaa agccgggccc
     3061 cgtgctggtc gatattccgc aggatgtcct cgcctccgac atcgaattct ccaattataa
     3121 gccggttgga aagcccgcga ggtcttgtgg cgatgccgtt cagatcaagg ctgccgccgc
     3181 cgcattgcgg tcggcaaagc ggcctttgat cgtttcaggc ggcggcgcca tcgcatccga
     3241 tgcagccggc gaagtgctac ggcttgcaga agcgctcgcg atccctgtcg ccacgacctt
     3301 ggcagggcgt ggcatcctgc ctgacgatca tgtgctggcc gccggtggat tggggcatca
     3361 ccgacaggag atcaccaagc gactgctgcc aaacgcggat gtcgtcctcg gcattggaac
     3421 ccggttcgag caacaggaaa ccaactggaa gccggactat ctgcctgccc cggcggcgac
     3481 ctacatccag atcgacgtgg acccggccga gattggtcgc tcggtggtgg ccacgatcgg
     3541 gattgtcggc gacgcaaagc tggttttgca ggatctgctg gccgagctgg atcaatcctg
     3601 cgttgccagc cggtggaacg atgcagatac gcacacgggt gccgacgacc tggcacggca
     3661 acgagctgcg ctggaatccg agatcagcga gatggtggcc tcggactctc ggccgcttca
     3721 tccgatccgg gtgatccgca aaattcgcga cgcttttccg cgtcatgcca cggtggccat
     3781 cgatgtcggt gtgctcgcgc aaggcatggg cggagctttt ccatatttca agatctacca
     3841 gccgcgctcc accatcgtac catcgagctt ttacggtatg ggtttttcgg catcggcgtt
     3901 tccggtggcg cggctggttt acccagacag gccggcggtc tgtctggttg gcgacggctc
     3961 gtttcagatg gtgatgaaca tcctgccggt ggcggccgag ctcaagctcg gtgtgacctg
     4021 gtgcatcctg aatgacggcg cgctcggttc gatcagggat atccaggccc aggcattcag
     4081 cgagcgctat atcggaacgg agttcgcgct ggatctcgac ttcgcaaaat tggcgcaggc
     4141 ttccggatgt tacggcgaac gcgtaacgga gcccgctgaa atcgaaaatg cgctcaagcg
     4201 cgcgattgat gcgaacgagg gcggcaggcc agccgtcctc gattttttcg tcgccaagga
     4261 acgtctcaaa ggcagcgtgg agtttttcaa gcgatgaaat ttctttcatt cgggcagccc
     4321 gtcgggggca tcgttcaggc cgcctacacg gtcgaaaaca tcgatcgtgc tatgagcgac
     4381 ttcacgcaac gcctgaacgt cgggccatgg ttcgtgaccg gcccatttac gccgagcaag
     4441 ggatattacc gggggcagcc caccgatatg agccttacgc tcgccgttgc attcgccggt
     4501 cacatgatga tcgagctgat cgagcaacac gacgacaagc cctcggtgta tcaggagacg
     4561 atcaagatca aggggcacgg ctttcatcac tgggcgatct gctcgaaatc gttcgatcag
     4621 gacgtcgcgc gctacgaggc tgccggctat ccgatcgcct tctccgatct ttcgccgcgc
     4681 ggtgttcgca tcgtctacat ggatacgacc cgagatctgc cgggaatgct cgagatcatc
     4741 gaggccacgg atgccttgga ggcgatctat caaagttact tcgatgcggc gcagggctgg
     4801 gacggcaggg acccggtgcg gcgctggtcg ctgaagaccg gggcatgaga tgaagacgca
     4861 agatctcttt gacgttgccg gtctgtccgt tctcatcacc ggcggtgcca aggggcttgg
     4921 ccgcgccttc gcggaggcct tgctcgacaa tggcgcgcgc gtaacattgc tcgactgcga
     4981 tggcgagtgt ctggatcggg ctgtcatcga actgcgcgac cgaggcaccg cggtacatgg
     5041 cgacgtggtc gatgttacgg atcgcgccgc gatgatgaag gccgtcgacc ggacggtcga
     5101 tcgcctgggc cggctcgacg tcgtgttcgc caatgccggg atcgacgccg gccccggctt
     5161 tctgtcgatg agcggcgagc gcgatccgca
//
LOCUS       DASWGM010000783         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_927571,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000783 DASWGM010000000
VERSION     DASWGM010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927571"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VGA15_21610"
     CDS             <1..190
                     /locus_tag="VGA15_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9590328.1"
                     /translation="RVATHCRSRGIGEQMVQWAIAEARAKECKLLELLTHNTRVDAQR
                     FYARLGFAPSHVGMTLRF"
     gene            562..945
                     /locus_tag="VGA15_21615"
     CDS             562..945
                     /locus_tag="VGA15_21615"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9590329.1"
                     /translation="MILSRKEYDRLLAAANEDVTDAAVAKKAIARNEETLSEAEMDEL
                     LAAKTPLAFWRKKRGLTQTDLAKAAEIAQGFLSEIESGLKTGDVTVLQRIAIALEISL
                     LELVPDLPPGKRKPGVKFNTVKRRK"
     gene            1505..>2551
                     /locus_tag="VGA15_21620"
     CDS             1505..>2551
                     /locus_tag="VGA15_21620"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEX9590330.1"
                     /translation="MTAAKPASRPNVPHFSSGPCAKRPGWNAQNLKDAALGRSHRAKI
                     GKAKLKLAIELTREVLEIPAGYRIGIVPASDTGAVEMALWSMLGARPVTTIAWESFGE
                     GWVSDIVKELKLKDVTKLHAGYGDIPDLSKADPASDIVFTWNGTTSGVRVPNADWISA
                     GREGLTICDATSAAFAQKLDWAKLDVVTFSWQKALGGEAAHGMLVLSPRAVERLDTYK
                     PAWPLPKIFRMTKGGKLNEGIFEGETINTPSMLCVEDYLDALNWGKSVGGLKGLMARA
                     DANTKVLADWKAKTPWIDFLAKDPAVRSNTSVCMKVVDPAITSLTLDAQADFAKKLVA
                     LVEKEGAGYDFAHYR"
BASE COUNT          567 a          747 c          766 g          471 t
ORIGIN      
        1 tcgcgtcgcc acgcattgcc gcagccgcgg cattggcgag cagatggtgc aatgggctat
       61 cgcggaagcg cgggcgaagg aatgcaagct gctcgaattg ctcacgcaca atacccgcgt
      121 cgatgcgcag cgattctacg ctcggctggg atttgcgccg agccatgtcg ggatgacgct
      181 acgattttga cagatcccaa aatgggataa acattcgtcc gatatctcaa taagagattg
      241 accagaatat ctcgatatga gataaatcga cggtgaaatc ggttctgtac acgcgaaccg
      301 cggcgacggc cctgcgcaag tacgcaaacc gagcgaaatt gattcggtcc aagatcagac
      361 aatatgccga cgatgcatcg tcgcaggcca acaacgtgaa atcgctggtc ggcgtcgatg
      421 cgaagcgctt acgggttggc agctatcttc accgaaacga ccgacaccat taccgtcctc
      481 gatattggcc cgcgcggcgg gatctacgaa taggagcggc ccatgcccgt gatgaccaag
      541 agcccaaagg gcgacgatat cgtgatcctc tcccgcaagg agtacgatcg gctgctcgcc
      601 gccgccaatg aagatgtgac cgatgcagcg gttgccaaaa aggcgattgc gcgcaacgag
      661 gaaacgctaa gcgaagccga gatggatgaa ctgcttgccg ccaaaacgcc gctcgcgttc
      721 tggcgcaaga aacgcggctt gacacagacg gatttagcga aggctgccga gatcgctcag
      781 ggttttctct ccgaaatcga gagcggcctc aagacaggag atgtcacggt ccttcagcgg
      841 attgcgatcg ctcttgaaat ttcgcttctc gaactggtgc cggaccttcc tccaggcaag
      901 cgcaaaccag gcgttaagtt caacaccgtc aagcgccgga aatgaataac gtctatccga
      961 cggccaccat ttcttcggag gagggcacca agcagcgacg tcgccggctg cgccgattgg
     1021 accgcccaaa tccggacggt ccttgcgatc attgtgaaac acggtttgcc attcgatccg
     1081 gttaaccggc agtaaactac tcagcccttc gtcgttttct tctccgtgcg aaccgtgtca
     1141 ccttgatggt gcagggcggc tcgggggtcg ttggggaccg cacatgaaag attacacgat
     1201 cgtccggatc ggcaacgaat atgtcgtgca ggcgcacgaa aaaagcgttc tgaaaatcgc
     1261 aagccggcga agagccgaaa gaacggtcac ggatgcggcc gagcttatgg attcgcaacc
     1321 cgctccgcca ttgtcgcagg atgcgccaga cgagccatca atcgcccgtg atcccggggt
     1381 catacctgat cccggcacca tacctgatcc atccgaagtt ccttgacgat tcagggtgat
     1441 tcccctaagc accgcggcgg gaaacctccc cccaccggga ggcagctatc tggaaggata
     1501 gatcatgact gcagcgaagc ccgcttcgcg gccgaatgtg ccgcatttct cctccggtcc
     1561 ctgcgccaag cgccccggct ggaatgccca aaatcttaaa gacgccgcgc tcggccgttc
     1621 gcatcgcgcg aaaatcggca aggcgaaact caagctcgcg atcgagttga cgcgcgaagt
     1681 acttgaaata ccagccggtt acaggatcgg catcgtgccg gcgtccgata ccggcgcggt
     1741 tgaaatggcg ctgtggtcga tgcttggcgc gcggcctgtg acgacgatcg cctgggaatc
     1801 atttggcgaa ggttgggtca gcgacatcgt caaggaattg aagctcaagg atgtgaccaa
     1861 gctgcatgcc ggctatggcg atatccccga cctgagcaag gccgatcctg cttccgacat
     1921 cgtcttcacc tggaacggca ccacctcggg cgtgcgcgtt cccaatgccg actggatcag
     1981 cgcgggccgc gaaggcctca ccatctgtga cgccacctcg gcggcattcg cccagaagct
     2041 cgattgggcc aagctcgatg tggtgacgtt ctcctggcag aaggcgctcg gcggcgaggc
     2101 cgcgcacggc atgctggtgc tgagcccgcg cgcggtcgag cggctcgata cgtacaagcc
     2161 ggcatggccg ctgccgaaaa tcttccgcat gaccaagggc ggcaagctca acgaaggcat
     2221 cttcgagggc gagaccatca acacgccgtc gatgctgtgc gtcgaggatt atctcgacgc
     2281 gttgaattgg ggaaaatcgg tcggcggtct caagggtttg atggcgcgcg ccgacgccaa
     2341 caccaaggtg ctcgccgact ggaaggcgaa aacgccgtgg attgatttcc tggcgaagga
     2401 tccggctgtg cgttccaata cttcggtgtg catgaaggtg gtcgatcccg cgatcacctc
     2461 gctgacgctg gacgcgcagg cggattttgc caagaagctg gtcgccctgg tggagaagga
     2521 aggcgccggc tacgatttcg cgcattatcg c
//
LOCUS       DASWGM010000784         4521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1182228,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000784 DASWGM010000000
VERSION     DASWGM010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182228"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VGA15_21625"
     CDS             complement(<1..1207)
                     /locus_tag="VGA15_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX9590331.1"
                     /translation="MRLLIVGTLKGQLTTATKIAMDNGASVTHAEDHEQAMRVLRGGK
                     GADLLLVDVALDIRDLVLRLEAEHIHVPIVACGISNDARAAVAAIHAGAKEYIPLPPD
                     PELIAAVLAAVANDSRDLIYRDEAMAKVIKLAQQIAGSDASVMITGESGTGKEVLARY
                     VHTRSNRAKKPFISINCAAIPEHLLESELFGHEKGAFTGAVARRIGKFEEATGGTLLL
                     DEISEMDVRLQSKLLRAIQERVIDRVGGTRPVPVDIRIIATSNRNLADAVREGTFRED
                     LLFRLNVVNLKIPPLRERPADILELAQHFAKKYADANGVPLRPISADARRVLTVNRWQ
                     GNVRELENTIHRAVLMAQGDEIGADAILTPDGDRLDLAKTVPAVAHATFAAEQVTRAL
                     VGRTVADVER"
     gene            complement(1262..1612)
                     /gene="fliN"
                     /locus_tag="VGA15_21630"
     CDS             complement(1262..1612)
                     /gene="fliN"
                     /locus_tag="VGA15_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX9590332.1"
                     /translation="MSDTDGQVPLPDLNAADAPSIDDVAYNEDEQASRVAADLEAVFD
                     VPVQVSAVLGRSKMDVGELLKLGPGTVLELDRRVGEAIDIYVNNRLVARGEVVLVEDK
                     LGVTMTEIIKAERT"
     gene            complement(1609..2250)
                     /locus_tag="VGA15_21635"
     CDS             complement(1609..2250)
                     /locus_tag="VGA15_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX9590333.1"
                     /translation="MAAPAKFLFDMDFSAPDKARERPATPSEISQKIASAEARAYHDG
                     YDTAQREAKAESDRRTALALEEIAIGIQGIATRFSGIETRMETEAVDVAVAVARKLCS
                     ELISGEPLVEISGLVSDCFSHLVSTPHLVVRINDSLYEAAHERIERLAKQSGFAGRLV
                     ILAEPEIANGDCRIEWADGGVVLERAAIEAKINELVGRYMASRGGTTTLRNEP"
     gene            complement(2250..3332)
                     /gene="fliG"
                     /locus_tag="VGA15_21640"
     CDS             complement(2250..3332)
                     /gene="fliG"
                     /locus_tag="VGA15_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX9590334.1"
                     /translation="MAVPQTTNAHDITSVIATLASRQKDRPKGPPLSGPKRAAILMLA
                     LGEQYGGKVWSLLDDDEVRELSMHMSTLGTVEADVVEDLLLEFVSRMSASGALMGTFD
                     GTERLLQLYLPADRVTGIMDEIRGPAGRNMWEKLSNVQEEVLANYLKNEYPQTIAVVL
                     SKLKPEHAARVLAILPEDMALDVVGRMLRMEAVQKEVIERVEQTLRTEFMSNLSQTRR
                     RDAHEVMAEIFNNFDRQTETRFITSLEEDNRESAERIKALMFTFDDLVKLDAASAQTL
                     MRHVDKDKLGIALKSANEDVRGFFLGNMSSRAGKMLLDDMAALGPVRLRDVDEAQALL
                     VNLAKDLAAKGEIMLTKNRADDELVY"
     gene            complement(3334..>4521)
                     /gene="fliF"
                     /locus_tag="VGA15_21645"
     CDS             complement(3334..>4521)
                     /gene="fliF"
                     /locus_tag="VGA15_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX9590335.1"
                     /translation="RVQAARVHLVLPERPLFSRETPEPSASIVVRVRGALEPQQIRAI
                     RHLVASAVNGLKPQRVSIVDEAGQLLADGAAGDADNAVGDERRAGFEKRMRNQVEAIV
                     SSVVGAGRARVQLSADFDYNKITQTSDKFDPEGRVLRSSQTREESSATAETNGQVTVN
                     NELPGNQRQDNTTPARDQSKKSEETNNYEISHTTKTEVTEVGRVNRISVAVLVDGSYV
                     KNDKGEMVYQERGKEQLDRIATLVRSAIGFDQKRGDQVEVVNLKFAEAPAVVPVAEPT
                     GLLGMLQFTKDDVMYIIELGVMMLLGLVVLFMVIRPLVKRILASEVIPALAHEHLPAL
                     TDGSDAAGLVPGPNATAQMIDVAQVQGQVHAQSVHRVGELAERNPNETATIIRQWLTE
                     PAN"
BASE COUNT          722 a         1428 c         1458 g          913 t
ORIGIN      
        1 cgcgctcgac gtccgcgacc gtacgtccga ccagtgcgcg ggtcacctgt tcggcggcga
       61 aggtggcgtg cgccaccgca ggcacggtct tggcgaggtc gagacgatcg ccgtccggcg
      121 tcaggatggc gtcggcgccg atctcgtcgc cctgcgccat cagcaccgca cgatggatgg
      181 tgttttccag ttcgcggacg ttgccctgcc agcggttcac cgtcagcacc cgacgggcat
      241 cggcggagat cgggcgcaac ggcacgccgt tggcgtcggc atatttcttg gcgaagtgct
      301 gcgccagttc gagaatgtcc gccgggcgct cgcgcagcgg cgggatcttc aggttcacca
      361 cgttgagccg gaacaaaagg tcctcgcgga acgtgccttc gcgcacggcg tcggcgaggt
      421 tgcggttcga ggtcgcgatg atgcggatat cgaccggcac cggccgggtg ccgccgaccc
      481 gatcgatcac gcgctcctgg atcgcgcgca gcaatttgga ttgcagccgc acatccattt
      541 cggagatttc gtccagcagc agcgtgccgc cggtggcttc ctcgaatttt ccgatccgcc
      601 gcgcaacggc gccggtgaac gcgcccttct cgtggccgaa caactcggat tcaagcaggt
      661 gctcggggat cgccgcgcag ttgatgctga tgaacggctt ttttgcgcgg ttggagcggg
      721 tgtggacata gcgcgccagc acttccttgc cggtaccgga ttcgccggtg atcatgacgg
      781 aggcgtcgga gccggcgatt tgctgcgcca gcttgatcac cttggccatt gcctcgtcgc
      841 ggtagatgag gtcgcgggag tcgttggcga cggcggcgag cacggctgcg atcaattcgg
      901 gatccggcgg cagcgggatg tattccttgg cgccggcatg gatcgcggcg accgcggcgc
      961 gcgcgtcatt ggagatgccg caggcgacga tcggcacgtg aatgtgttcc gcctccagcc
     1021 gcagcaccag gtcgcggatg tcgagagcga cgtcgaccag caacaggtcg gcgcccttgc
     1081 cgccgcgcaa cacgcgcatc gcctgttcgt ggtcctccgc gtgggtcacg gaagcgccat
     1141 tgtccatggc gatcttggtg gccgtcgtaa gctggccctt cagtgtgcca acgatgagaa
     1201 gccgcatttt aatctcctgt tcgtctgttc gcgccagtca tgcgccgtcg ctagataacc
     1261 tttaggtgcg ttccgccttg atgatctccg tcatggtcac gccaagcttg tcttccacca
     1321 gcacgacctc gccgcgcgcc accagccggt tgttgacata aatgtcgatg gcttcgccga
     1381 cgcgacgatc gagttcgagc acggtaccgg gcccgagttt cagcaattcg ccgacgtcca
     1441 tcttggaacg gccgagcacg gccgaaacct gcaccggcac gtcgaacacg gcttccaagt
     1501 cggctgcaac ccgcgaagcc tgttcgtcct cgttatacgc cacgtcatcg atcgacggcg
     1561 cgtcggcggc attgagatcc ggaagcggaa cctgtccgtc ggtgtcactc atggttcatt
     1621 ccttaaggtc gtagttccac cgcgggacgc catgtagcgc ccgacaagtt cgttgatctt
     1681 ggcttcgatc gccgcgcgct ccagcacgac gccgccgtcg gcccactcga tcctgcaatc
     1741 gccgttcgcg atttccggtt ctgccaggat cacgaggcgg cccgcgaaac cgctttgttt
     1801 cgccagccgc tcgatccgct cgtgtgcggc ctcatacagg gaatcgttga tccggaccac
     1861 gagatgcggc gttgagacca ggtgggaaaa gcaatcgctc accagcccgg agatctcgac
     1921 cagcggctcg ccggaaatca actcgctgca cagcttgcgc gccaccgcga ccgcgacatc
     1981 gacggcttcg gtctccatcc gggtttcgat gccggagaac cgcgtcgcga taccttgaat
     2041 gccaattgcg atctcctcga gcgccagcgc agtgcggcgg tcgctttcgg cttttgcctc
     2101 gcgctgcgcg gtatcgtaac cgtcgtgata ggcgcgggcc tcggcggatg cgatcttctg
     2161 cgagatttcc gaaggcgtgg cgggccgttc gcgcgccttg tctggcgcgg agaagtccat
     2221 gtcgaacagg aactttgcgg gggcggccat cagtacacca gctcgtcgtc ggcgcggttc
     2281 ttggtcagca tgatctcacc cttggccgcg aggtcctttg cgagattgac gagcagcgcc
     2341 tgcgcctcgt cgacatcgcg caatcgtacc ggtcccagcg ccgccatgtc gtcgagcagc
     2401 atcttgccgg cgcgggacga catgttgccg aggaagaagc cgcggacatc ctcgttggcg
     2461 ctcttcaggg cgatgccgag cttgtccttg tcgacgtggc gcatcagggt ctgcgccgag
     2521 gcggcgtcca gcttgacgag gtcgtcgaag gtgaacatca gcgccttgat gcgttcggcg
     2581 gactcgcggt tgtcttcctc cagcgaagtg atgaagcggg tttcggtctg gcggtcgaaa
     2641 ttgttgaaga tttcggccat cacctcgtgg gcgtcgcgac ggcgggtctg cgacaggttc
     2701 gacatgaatt cggtgcgaag cgtctgttcg acgcgctcga tcacttcctt ctgcaccgcc
     2761 tccattctca gcatgcggcc gaccacatcg agcgccatgt cttcgggcag gatcgcaagc
     2821 acccgggcgg cgtgctccgg cttcagcttc gacagcacca cggcgatggt ctgcggatat
     2881 tcattcttga gatagttggc gagcacctct tcctgaacgt tggagagttt ctcccacatg
     2941 ttgcggcccg cggggccgcg aatttcgtcc atgatgccgg tcacgcgatc ggcgggcagg
     3001 tacagctgca gcagccgttc ggtgccgtcg aaagttccca ttaacgcgcc ggatgccgac
     3061 atccgcgaga cgaattcgag cagcaggtct tccaccacgt cggcttcgac ggtacctagc
     3121 gtcgacatgt gcatcgacag ttcgcgtact tcgtcgtcgt cgagcagcga ccagaccttg
     3181 ccgccatatt gttcgccgag cgccagcatc aggatcgcgg cgcgcttggg tccgctcaga
     3241 ggagggcctt tcgggcggtc cttctgacgg ctcgccagcg tggcgatgac gctggtgata
     3301 tcgtgggcgt ttgtggtttg cggtacagcc atgtcagttc gccggctcgg ttagccattg
     3361 acgaataatg gtagcggtct cgttcgggtt tcgctcggcc agttcgccga cgcgatgcac
     3421 cgactgcgca tggacctggc cctgaacctg ggcgacatcg atcatctgcg ctgtggcatt
     3481 ggggccggga acgaggccgg cggcgtcgct accgtcggtc aacgccggca aatgttcgtg
     3541 cgcaagcgcg gggatcactt ctgaagcaag gatgcgcttg accagcggcc ggatcaccat
     3601 gaacaacacc acgaggccga gcagcatcat gacgccgagt tcgatgatgt acatcacgtc
     3661 gtccttggtg aactgcagca taccgagcag tccggtcggc tcggcgaccg gaacgacggc
     3721 aggcgcttca gcgaatttaa ggttcacgac ctcgacctga tcgccacgct tctgatcgaa
     3781 gccgatcgcc gagcgcacca gcgtcgcgat gcggtcgagc tgttccttgc cgcgttcctg
     3841 atagaccatt tcgcctttgt cgttcttgac atagctgccg tcgaccagca ccgcgaccga
     3901 gatgcggttg acccggccga cctcggtcac ctcggtcttt gtggtatggg agatttcgta
     3961 gttgttggtc tcttcgctct tcttgctctg gtcgcgcgcc ggcgtcgtat tgtcctggcg
     4021 ctgattgccc ggcaattcgt tattgacggt gacctgaccg ttggtttcgg cggtggcgct
     4081 ggattcttcc ctggtctggc tcgaacgcag cacccggcct tcggggtcga acttgtccga
     4141 ggtctgggtg atcttgttgt agtcgaagtc cgccgagagc tggacgcggg cgcgtcccgc
     4201 tccgaccacc gatgaaacaa ttgcctcgac ctgattgcgc atccgcttct cgaagccggc
     4261 tcggcgctcg tcgccgacgg cgttgtcggc gtcgccggcc gcgccgtcgg cgagcaattg
     4321 cccggcttca tcgacgatcg acacccgctg cggcttcaag ccgttgacgg cggaggcgac
     4381 cagatggcgg atggcacgga tctgctgcgg ctcgagcgcg ccgcgcacgc gcaccacgat
     4441 cgaggccgat ggttcgggcg tctcgcgcga aaacagcggc cgctcgggca gcaccagatg
     4501 gacgcgggcg gcctggacgc g
//
LOCUS       DASWGM010000785         3133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2317377,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000785 DASWGM010000000
VERSION     DASWGM010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317377"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /gene="gmd"
                     /locus_tag="VGA15_21650"
     CDS             <1..939
                     /gene="gmd"
                     /locus_tag="VGA15_21650"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX9590336.1"
                     /translation="VDHLYEDPHAGNVPFLLHYGDMTDSTNLIRLMQQIRPTEIYNLA
                     AQSHVGVSLESPEYTANADAIGVLRLLEAIRILGMEKETRFYQASTSELYGLVQEVPQ
                     KETTPFYPRSPYGVAKLYGYWITVNYREAYGMFASNGILFNHESPIRGETFVTRKITR
                     SVARIETGLEETLYLGNLGAKRDWGHARDYVEGMHRILQAEAPDDFVLATGETRSVRE
                     FVEIAFAEVGRSIEWRGKGIEETGVDKKSGKTVVRIDPQYFRPTEVDLLIGDASKARE
                     KLGWKPTTTFAQLVKEMVAGDLAIARREVANGKNPV"
     gene            920..1879
                     /locus_tag="VGA15_21655"
     CDS             920..1879
                     /locus_tag="VGA15_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX9590337.1"
                     /translation="MARTPFELKGKTVFVAGHGGMVGAALVRRLAQEDVELLTVKRRE
                     LDLRDQAAVFAWFADKRPQAVFLAAAKVGGIVANNTLRGEFLYDNLIIAANVIHAAHV
                     NGTEKLMFLGSSCIYPKLAPQPLREDSMLTGPLEPTNEPYAIAKIAGIKMVEAYRSQF
                     GSDFINVMPTNLYGRGDNYHPEYSHVVAALIRRFHEAKLSGASNVVVWGTGSPRREFL
                     YVDDLADACIHLMKIYSGDGLVNIGTGEDITIAEFARVVAATVGYAGEISFDTSRPDG
                     TPRKLLDVSRLAGLGWRARTPLEEGIKLAYQAYLAEHHSRSVS"
     gene            complement(2075..2920)
                     /locus_tag="VGA15_21660"
     CDS             complement(2075..2920)
                     /locus_tag="VGA15_21660"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX9590338.1"
                     /translation="MADRLNGYRILILETREEAQFSRLLAEQGADVLQCPMFTIRDAP
                     DAAPIEAWIRRFVEKPCDDLVLMTGEGLRRLMKVARRIGADRDFIAALGNARKFARGP
                     KPGRALREIGLEPQVTTEKPTSDGIAEMLAQVDLSGHRVGLQLYPDKNHSVLIGAITA
                     QGAEVDPVLPYIYDAQAADANIVTAIDEMAQGRIDAIALTSSGQVRRLVEVAQAHRCE
                     VLLRDGLARTPIASVGPVVSDELTSHGLRTDIYPANDAYFMKPLISAMAAALGKTAPR
                     AAAKT"
BASE COUNT          581 a          979 c          978 g          595 t
ORIGIN      
        1 gtcgaccacc tctacgaaga tccgcacgcg ggcaatgtgc cgttcctgct gcattatggc
       61 gacatgacgg attcgaccaa cctgatccgc ctgatgcagc agatccggcc gaccgaaatc
      121 tacaacctcg ccgcccaaag ccatgtcggc gtcagtttgg agagcccgga atacaccgcc
      181 aatgccgacg cgatcggcgt gctgcggcta ttggaagcga tccggattct cggcatggaa
      241 aaagagacgc ggttctatca ggcgtccacc tcggagttgt acggcctggt gcaggaagtt
      301 ccgcagaagg agacgacgcc attctatccg cgctcgccct atggcgtcgc caagctctac
      361 ggttactgga tcacggtgaa ctatcgcgag gcctatggca tgttcgcctc gaacggcatc
      421 ctgttcaatc atgaaagccc gatccgcggc gagaccttcg tgacgcgcaa gattacgcgc
      481 agcgttgccc gcatcgagac cggccttgaa gagacgctct atctcggaaa cctcggggcg
      541 aaacgcgact gggggcacgc cagggattat gtcgagggca tgcacagaat attgcaggcg
      601 gaggcgcccg acgacttcgt gctggcgacc ggcgagaccc gctcggtgcg cgaattcgtc
      661 gagatcgcgt tcgccgaagt tggccgcagc atcgaatggc gcggcaaggg catcgaggaa
      721 accggtgtcg acaagaaatc cggcaagacc gtcgttcgca tcgatcccca gtatttccgc
      781 ccgaccgaag tcgatctcct gatcggcgac gccagcaagg cgcgcgaaaa gctcggctgg
      841 aagccgacga cgacgttcgc ccaactcgtc aaggagatgg tggcgggcga tcttgcgatt
      901 gcccggcggg aggtcgccaa tggcaagaac cccgtttgag ctgaaaggca aaacggtctt
      961 cgttgccggg catggaggga tggtcggggc tgcgctggtg cgcaggctcg cgcaggaaga
     1021 tgtggaactg ctgacggtca agcgcaggga actcgatttg cgcgatcagg ccgcggtgtt
     1081 tgcctggttt gccgacaagc gaccgcaggc ggtatttctg gcggcagcga aggtcggcgg
     1141 catcgtcgcc aacaacacgc tgcgcggtga attcctctac gacaatctga tcatcgcggc
     1201 caatgtgatc catgccgcgc atgtcaatgg caccgagaag ctgatgtttc tcggctcgtc
     1261 ctgcatctat cccaagcttg cgccgcagcc gttgcgcgaa gattccatgc tgacgggacc
     1321 gctggagccg accaacgagc cctatgccat cgccaagatc gccggcatca agatggtcga
     1381 agcctatcgc agccagttcg gatccgactt catcaatgtg atgccgacca atctgtacgg
     1441 tcgcggcgat aattatcacc ccgaatacag ccatgtcgtc gcagccctga tccgccgctt
     1501 tcatgaagcc aagctttccg gcgcctcgaa tgttgtggtc tgggggaccg gttcgccgcg
     1561 gcgggaattc ctgtatgtcg acgatctcgc cgacgcctgc attcatttga tgaagatcta
     1621 ttccggcgac ggactggtca atatcggcac cggcgaagac atcaccatcg ccgaattcgc
     1681 ccgcgtggtc gccgcgaccg ttggttatgc cggcgagatc agcttcgaca cctcgcggcc
     1741 cgacggcacg ccgcgcaaac tgctcgacgt cagccgtctt gccgggcttg gctggcgcgc
     1801 ccgcaccccg ctcgaggaag gaatcaagct cgcctatcag gcgtatttgg ccgagcatca
     1861 ttcgagaagt gtcagttagt ttcgtcattc cccgatgtgc aattgcacat ctgaggatgc
     1921 gccacttggc gcaggcccgg aatccatact ccctgcggtg gttatggatt ccgggctcgc
     1981 ggcggagcct gtcatcgggc cgcgcttcgc gcggacccgt tggcgcgccc cggaatgacg
     2041 gtgcacaaga cagcagtcgt caggtcttca gtcgtcaggt cttggccgcc gcgcgcggtg
     2101 cggtcttgcc aagtgcggcc gccatcgccg atatcagcgg cttcatgaaa taggcgtcgt
     2161 tggcgggata aatgtcggtg cgcagaccgt gagacgtgag ttcgtccgac acgaccggcc
     2221 ccacggacgc gattggcgtg cgcgccagcc cgtcgcggag cagcacttca caccggtgtg
     2281 cctgcgccac ctcgacgagg cggcggacct ggcctgaact cgtcagcgca atggcgtcga
     2341 tgcggccttg cgccatctcg tcgatcgcgg tgacgatatt ggcgtcggcg gcctgcgcgt
     2401 cgtagatata gggcagcacg ggatcgacct cggcgccttg cgccgtgatg gcgccgatca
     2461 atacgctgtg attcttgtcg ggataaagct gcaagcccac gcgatgcccg ctgaggtcga
     2521 cctgcgccag catctcggcg atgccgtcgg aggtcggctt ctccgtcgtc acctgcggct
     2581 ccagcccgat ttcgcgcaag gcccggccgg gcttcgggcc gcgcgcgaat ttccgtgcgt
     2641 tgcccagcgc ggcaatgaaa tcccggtctg cgccgatccg ccgcgcgacc ttcatcaggc
     2701 ggcggaggcc ttcgccggtc atcagtacca gatcgtcgca tggcttttcg acaaaacgcc
     2761 ggatccaggc ctcgatcggc gcggcgtcag gcgcgtcgcg aatggtgaac atcgggcatt
     2821 gcagcacatc cgcgccctgt tcggccagca ggcgggaaaa ctgcgcctcc tcgcgcgttt
     2881 ccaggatcag aatgcggtag ccgttcagtc tgtcagccat gatcttgcct aacgccgatc
     2941 gcctctggtt tgttcatcat ggcgcttggg ccgggattgg cgcaagggcg tcgcgggtga
     3001 tagagcaggg ccgcaaatca cccgttccat cctcatcctt cgattgtagc gaagcaccgc
     3061 catgcccgac catcaattcg tgttgacact gtcctgtccg gatcgtcccg gcatcgtatc
     3121 ggcggtgtcg acg
//
LOCUS       DASWGM010000786         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_491889,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000786 DASWGM010000000
VERSION     DASWGM010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491889"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGA15_21665"
     CDS             <1..439
                     /locus_tag="VGA15_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9590339.1"
                     /translation="ETIGSVEVAKATREAPSRIPFLLKDPRGHADFYDSLARHDSMGS
                     ANTMRSFQGGRPSIYTMSDAIQKVPTPTLIICGDEDDNCVEPSLFLKKHLPAAGLAIF
                     PKSGHVLNLEEPALFNEMVERFIALTEAGHWPVRDPRSMMIQT"
     gene            complement(551..1219)
                     /gene="metW"
                     /locus_tag="VGA15_21670"
     CDS             complement(551..1219)
                     /gene="metW"
                     /locus_tag="VGA15_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX9590340.1"
                     /translation="MTMQQQALPLTGMSPQDTGHYRGDHLLVAQMVERGSKVLDVGCG
                     DGDLLQLLESLGIDGRGIELSREGVNRCVAKGLAVVQGDADTDLINYPDDAFDYVILS
                     QTLQATRQPRVVLENLLRIGRRAIVSFPNFGFWKMRLQLLIGGHMPRTENLPATWYDT
                     PNIHFCTIKDFVQLCDEINVKMERAVALDLYGRPLRLNAPWWFWNMFGEQGVFLLSRA
                     EKTK"
     gene            complement(1221..>1628)
                     /gene="metX"
                     /locus_tag="VGA15_21675"
     CDS             complement(1221..>1628)
                     /gene="metX"
                     /locus_tag="VGA15_21675"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084216.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX9590341.1"
                     /translation="PTFSFDADFQVESYLRHQGSSFVERFDANSYLYLTRAMDYFDIA
                     ADHNGVLAKAFRGIQTRFCVVSFTSDWLFPTSESRALVHALNASSARVSFAEIETDRG
                     HDAFLLDVPEFFDISRAFLQSAGAARGLTRTGG"
BASE COUNT          340 a          503 c          493 g          292 t
ORIGIN      
        1 cgagacgatc ggctcagtgg aggtggcaaa ggcgacgcgc gaggcgccga gccggattcc
       61 gttcctgctg aaggacccgc gcggccatgc ggatttctac gactcgctgg cccggcacga
      121 ttccatgggc tcggccaaca ccatgcgcag ctttcagggc ggcaggccct cgatctacac
      181 gatgagcgac gccattcaaa aagtcccgac gccgacttta atcatctgcg gcgacgagga
      241 cgacaattgc gtcgagccca gcctgttctt gaagaagcat cttccggcgg ctggtttggc
      301 gatatttccg aaatcgggcc atgtgctcaa tctcgaggag cccgcgctgt tcaacgaaat
      361 ggtcgagcgc ttcatcgccc tgaccgaggc cggacactgg ccggtacggg atccaagatc
      421 gatgatgatt caaacctagc cgtcattccg gggcgtgcgt aagcacgaac ccggaatctc
      481 gagattcccc gatgtgcaat tgcacatctg aggtctggtc cttcggacca tcccggaatg
      541 acgatggagg ctacttcgtc ttttccgccc ggctcagcaa gaacacgccc tgctcgccga
      601 acatgttcca gaaccaccag ggcgcgttga gccgcagcgg acggccgtaa agatcgagcg
      661 cgacggcgcg ctccatcttg acgttgatct cgtcgcagag ctgcacgaag tccttgatgg
      721 tgcagaaatg gatgttgggc gtgtcgtacc aggtcgctgg cagattttcg gtgcgcggca
      781 tgtgcccgcc gatcaatagc tgcagtcgca ttttccagaa gccgaaattc gggaacgaca
      841 cgatggcgcg ccgaccgatc cgcagcagat tctccagcac cacgcgcggc tgccgcgtcg
      901 cctgcaaggt ctgcgacagg atcacataat cgaaggcgtc gtcgggataa ttaatcaggt
      961 cggtgtcggc gtcgccctgc accacggcaa gacccttggc gacacaacga ttgacgccct
     1021 cgcgcgacag ctcaatgccg cggccgtcga tacccaggct ttccagcagt tgcagcagat
     1081 cgccatcgcc acagccgaca tcgagcactt ttgagccgcg ctcgaccatc tgcgcgacca
     1141 gaagatgatc gccgcgataa tgcccggtgt cctgcggcga catgccggtc agcggcaaag
     1201 cctgttgctg catggtcatg tcagccgcca gtcctggtca ggccgcgcgc cgcgcccgcc
     1261 gattgcagga aagcgcgcga gatatcgaaa aattcgggca cgtcgagcag gaatgcatca
     1321 tggccgcggt cggtttcgat ctccgcgaac gatacccgcg cgcttgaggc gttgagcgca
     1381 tgcaccagcg cgcgcgactc cgaggtcggg aacagccagt cgctggtgaa cgacaccacg
     1441 cagaaccggg tctggatgcc gcgaaaggct tttgccaata cgccgttgtg atcggcggca
     1501 atgtcaaaat agtccatcgc ccgcgtcagg tagagatagc tgttggcgtc gaaccgctcg
     1561 acaaaggacg aaccctggtg gcgcagatag ctctcgacct gaaaatccgc gtcgaacgag
     1621 aacgtcgg
//
LOCUS       DASWGM010000787         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_99601,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000787 DASWGM010000000
VERSION     DASWGM010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99601"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..84)
                     /locus_tag="VGA15_21680"
     tRNA            complement(9..84)
                     /locus_tag="VGA15_21680"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49..51),aa:Val,seq:tac)
     gene            complement(148..621)
                     /locus_tag="VGA15_21685"
     CDS             complement(148..621)
                     /locus_tag="VGA15_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590342.1"
                     /translation="MRTRFIMAASAALVTVLAWSPPVLAQQKTIKQCRDEWSANKVAI
                     AASGKTQRVFVAECRGVPLVARTAPIAELAKGQYATEAEAKVSCRSDAVVWVNLRSKA
                     YHDSGSKSYGATKTGAYMCEKESIAAGFHAAKSAKEAAKESARKPVKELAKPASS"
     gene            746..1815
                     /locus_tag="VGA15_21690"
     CDS             join(746..1250,1250..1815)
                     /locus_tag="VGA15_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611116.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9590343.1"
                     /translation="MPAAVSITREDYTAAELRREAKRTDDADVSRRMLALALVLEGKS
                     RGEAAISCGMDRQVLRDWVHRYNAEGVKGLHNRIAPGAKSKLSHEQQREVAELVRKGP
                     DLAEHGVVRWRRIDLSRVIEQRYGVKLAERSVGDLLRRLGFRRISVRPRCPEQDAATQ
                     EAHKKTFPNLVAACIPAHARGKPIELWWQDEARVGQQGTLTRVWAERGSRPRAPRDQH
                     YDWAYLFGAVCPARDAGAALVLPVADGETMSLHLAEISRNVMPGSHAVVLLDGAGWHQ
                     TGGKLHVPDNISLLKLPAYSPELNPVENVWQYLRQNQLSNRVFDSYDDIIDACCDAWN
                     ALTAEPGRIRSIATRAWASVNL"
     gene            complement(2006..>2882)
                     /locus_tag="VGA15_21695"
     CDS             complement(2006..>2882)
                     /locus_tag="VGA15_21695"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9590344.1"
                     /translation="VARIICLIAAGGGIDATARIVAAQLSQIWGQQVMVENKTGASGN
                     TAAEFVARSDPDGYTIYIAAFPHATNRYLYASLRYDPVADFAPVTLIGLYPLVMVVPN
                     SSPAHSVREFIAYAKTNKLSYASSGHGTSLHLAGELFQRKAGIEMTHVPYRGAGPAFN
                     DLIPGRVDVMINFTSSSLPLVRQGQLRALAVTTVKRLSVAPEVPTMAEAGVAGMEVSS
                     WSAFFVPARTPREIIRKIHTDTVTALVEPAVREKLEQGGVVVIGSTPDELASFLSSEM
                     QRWGQVIKEANIRTQ"
BASE COUNT          546 a          869 c          893 g          574 t
ORIGIN      
        1 gcgaaggctg gtgggcggtg acggactcga accgccgaca tcctgcgtgt aaagcaggcg
       61 ctctgccagc tgagctaacc gccctcgcga ccacctttat cgccgccgcc aacggcgagg
      121 caagcgctcg ccggccgaaa cctggcatca gctggaagcc ggtttggcga gttccttgac
      181 cggctttctt gcagactctt tcgcagcctc cttcgcagac ttggcggcgt ggaagccggc
      241 tgcgatcgat tccttctcgc acatataggc gccggtcttg gtcgccccgt aactcttgct
      301 gccgctgtcg tgataggcct tggaccgcag attgacccag acgaccgcat cgctgcggca
      361 gctgaccttg gcctcggctt cggtcgcata ttgccccttg gcgagttcgg cgatcggcgc
      421 cgtccttgcc accaggggaa cgccgcgaca ttccgctacg aaaactcttt gcgttttccc
      481 gctcgccgca atcgcgacct tgttggcgct ccattcgtcc cggcactgct tgatcgtctt
      541 ctgctgcgcc aacacgggcg gcgaccaagc caggacagtg accaatgcag cgctcgcagc
      601 catgatgaac ctcgttctca tcgcacctct ccaagtatgt tgcgccggac taataccagc
      661 ggcgttgaat tctgactcat aggggattcc gtacagcttg cgggcatgat tcgcttggcc
      721 ttcttgattc gcggaggagg caagcatgcc ggcagcggtt tcaatcacgc gcgaggacta
      781 tacggcggcg gagcttcgtc gcgaggcgaa acggactgat gacgccgatg tttcccgccg
      841 aatgttggcg ttggcgctgg tgttggaagg caagtcgcgc ggggaagctg cgatatcgtg
      901 cggcatggat cggcaggttc tgcgcgactg ggtgcaccgc tacaatgccg aaggcgtgaa
      961 gggtcttcac aatagaattg cgcccggagc gaagtcgaaa ctgtcccacg agcagcaacg
     1021 agaggtggcc gagttggttc ggaaagggcc ggatctagca gaacatggcg tggtgcgctg
     1081 gcgacggatc gacctgtctc gcgtgattga gcaacgctac ggggtcaagc tcgccgaacg
     1141 gtcggtcggc gatctgctcc gccgtctcgg cttccggcgc atatcggtgc ggccgcgctg
     1201 tcccgagcag gatgccgcga cccaggaggc gcataaaaaa actttcccga tctggtcgcg
     1261 gcctgcatcc cggcgcacgc gcgcggcaag ccgatcgagc tttggtggca ggatgaggcc
     1321 cgtgtcggtc agcaaggcac gctgacccgg gtgtgggccg agcgcggcag ccgaccgcgg
     1381 gcgccgcgcg accagcacta cgactgggcc tatctgttcg gcgctgtctg tcccgcccgc
     1441 gatgccggcg ccgcgctcgt cctgccggtg gccgacggtg agacgatgag cctgcatctg
     1501 gccgagatca gccgcaacgt catgccaggc agccatgccg tggtgctgct cgatggcgct
     1561 ggctggcatc agacaggcgg caagctccat gtgccggaca atatcagcct gctcaagctg
     1621 ccggcctatt cgcctgaact caatccggtc gagaatgtct ggcaatattt gcgccagaac
     1681 cagctcagca atcgcgtctt cgacagctac gatgacatca tcgacgcctg ctgcgatgct
     1741 tggaacgcac tcaccgccga accaggcaga atccgatcaa tcgctactcg cgcctgggca
     1801 tcggttaatc tctaatgccg ttggtataat accaatggtg tcagataatg actctcgatc
     1861 gtcatcgtcc gcgaaagcgg acgacccagt aaaccgcagc gtatcagtat aacttcgacg
     1921 attgtgattt actggatacc cgctttcgcg ggtatgacag gtcagagttt ggtgccgttg
     1981 gcataaggta agcctgctgg cagcttcact gggttctgat gttcgcctcc ttgatgacct
     2041 gtccccatct ctgcatctcg gagctcagaa aggaggcaag ctcatccggc gtggagccga
     2101 tgaccacgac accgccctgt tccagctttt ctctgactgc tggctcaacg agggctgtaa
     2161 ccgtatcggt gtgaattttt ctgatgattt ctcggggtgt cctggcggga acaaagaatg
     2221 ccgaccatga agaaacttcc attcctgcaa caccggcctc cgccatcgtc ggcacctcag
     2281 gcgcgactga tagccgtttg acggtggtca ccgcgagcgc ccgcagttgg ccttggcgta
     2341 ccagcggcag gctcgacgac gtgaaattga tcatgacatc gacgcggccc gggatgaggt
     2401 cattaaaggc cggtcccgct ccgcgatagg gcacgtgcgt catttcaata ccggctttgc
     2461 gctgaaacag ctcgccagca aggtgcagcg acgttccatg gcctgaggac gcatagctaa
     2521 gtttgttcgt cttcgcgtag gcgatgaact ccctcaccga gtgcgccgga gaggagtttg
     2581 gcacgaccat cacgaggggg tagaggccga tcagcgtgac gggggcgaag tcggcgaccg
     2641 gatcgtatct gagcgaggca taaagatagc gattggtggc atgcgggaac gcagcaatat
     2701 aaatggtgta accatcaggg tcggaacgcg cgacgaattc ggccgcggtg ttaccgctgg
     2761 caccagtctt gttctcgacc atgacctgct ggccccaaat ctgggatagc tgggcggcga
     2821 cgatacgggc agtcgcgtcg attcctcccc cggccgcgat caggcagatg atgcgcgcaa
     2881 cc
//
LOCUS       DASWGM010000788         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_769076,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000788 DASWGM010000000
VERSION     DASWGM010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769076"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGA15_21700"
     CDS             <1..289
                     /locus_tag="VGA15_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590345.1"
                     /translation="ALALIFAVFLTNPSPICVLDEVDAPLDDHNVERFCNLLHEMTAS
                     TETRFIIITHNPITMARMNRLFGVTMAERGVSQLVSVDLEDAVKILDQDVA"
     gene            506..1507
                     /locus_tag="VGA15_21705"
     CDS             506..1507
                     /locus_tag="VGA15_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEX9590346.1"
                     /translation="MTTPDLPAELKAALDARLQGRSRNDAAGRAAAISQTYRDGGGSG
                     GITSEADALAYALVRMPATYAAVTASLNALGETRPELAPASLLDVGAGPGTATWAAME
                     AFSSLTHFTLLDANSALRALALALCSGSARLRDMTYQRGEARAALTDAKAADLVIASY
                     MIGELDDAEREALADLMWAKTRDTLLVVEPGTPAGYAGIIALRQRLIASGAHVAAPCP
                     HDGACPLAAPDWCHFTQRLPRSRAHKQLKSAELPYEDEKFIYVALTRAAIAQRPARVL
                     AQPVVSKVEVAAKLCTPAGLVIANVPRRAKADYAQARRWRWGDAVMETEAFAPGRPR"
     gene            1677..2237
                     /locus_tag="VGA15_21710"
     CDS             1677..2237
                     /locus_tag="VGA15_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX9590347.1"
                     /translation="MSSSWIVIGVIVILVLFAFAAYNRLVALSQRVGQSFADIDVQLK
                     QRHDLIPNLLETVKGYAAHERGTLDDVVKARNAAMSAQGPGQVAAAENQLSGALGKLI
                     ALSEAYPDLKANANFQQLASELSDLENKIAASRRFFNNAVQEYNTGIQQMPAALFAGM
                     FGFTRKDFFDLGANRAEVEAVPTVKF"
     gene            2270..>2763
                     /locus_tag="VGA15_21715"
     CDS             2270..>2763
                     /locus_tag="VGA15_21715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX9590348.1"
                     /translation="MAAYGLYTHIASNKFRSMLLLAGLFLLIYVLVYAGALVAEVVID
                     SGESADTYLAAAGRDLVTAFPYATGAAVLWIVIAYFFHQSIVDAVTGGEDVTRQQQPR
                     LYNLLENLCILRGIPMPKLKVMDSEALNAFATGLNQRQYAVTVTSGLLKALNDQEIEA
                     VLGH"
BASE COUNT          511 a          899 c          867 g          486 t
ORIGIN      
        1 cgcgctggcg ctgatcttcg cggtgttcct caccaacccg tcgccgatct gcgtgctgga
       61 cgaggtcgac gcgccgctcg acgatcacaa cgtggagcgg ttctgcaacc tgctgcacga
      121 gatgaccgct tcaaccgaaa cgcgcttcat catcatcacg cacaatccga tcacgatggc
      181 gcggatgaac cggctgttcg gcgtcaccat ggccgaacgc ggcgtatcgc aactggtctc
      241 ggtcgacctc gaggatgccg tgaagattct cgatcaggac gtggcgtgat ctcgcttttc
      301 ccctctcccc ttgtgggaga gggtggtcgc gatgcgaagc atcgcggacg ggtgaggggt
      361 tacggtctct cgacctgaac aagacccctc atccggcacg gacttcgcgt agccgatgca
      421 aagcatcggc gtcctatgat agcacggcgg ccgaaggccg cctacggcca ccttctccca
      481 caaggggaga agggaagaag acgaaatgac cactcccgac cttccagcgg aactcaaagc
      541 cgccctcgac gccaggctgc agggccgctc gcgcaatgat gctgccggac gcgccgcggc
      601 gatctcgcag acctaccgcg acggcggcgg ttccggcggc atcacgtccg aggccgatgc
      661 gctggcttat gcactggtgc ggatgcccgc gacctatgcc gcggtgaccg caagcctgaa
      721 cgcgctcggt gagaccaggc cggagttggc gccggcaagc ctgctcgacg tcggcgccgg
      781 gccgggaacg gcgacatggg ccgcgatgga agcattctcg tcgctgacac attttacgct
      841 gctggatgcc aacagcgcgt tacgcgcgct ggcgctcgct ctttgcagcg gcagcgcgcg
      901 gctacgcgac atgacatacc agcgcggcga ggcgcgcgcg gcgctgacgg acgcaaaagc
      961 tgccgacctc gtcatcgcga gctacatgat cggcgagctc gatgatgcgg aacgagaagc
     1021 actcgctgat ctgatgtggg caaagacccg cgacacgctg ctggtcgtcg aacccggcac
     1081 accggcgggc tatgctggga tcatcgcgct gcgccaacgg ctgatcgcgt cgggcgcgca
     1141 tgtcgcggcc ccctgcccgc acgacggcgc gtgcccgctg gcggcgccgg actggtgcca
     1201 cttcacacaa cgcctgccgc ggtcgcgcgc gcataagcaa ctcaaatccg cagaacttcc
     1261 ctatgaggat gaaaagttca tctatgtcgc gctgacgcgc gcggcgatcg cgcaacgccc
     1321 cgcccgtgtg ctggcacagc ccgttgtcag caaggtcgaa gtggcggcga aactgtgcac
     1381 accggccggt cttgtcattg caaatgtacc ccgccgcgcc aaggcggact atgcgcaagc
     1441 ccggcgctgg cgatggggcg atgcagtgat ggagacggaa gcgtttgccc cgggtcgtcc
     1501 ccggtgaacg ccgttcgcct ggcgttcgcc gggacaaccc agcggggtag ccttcccctt
     1561 gaacttatcc cggcctgcga ccgaccaagg agatacccaa ttcgcggcgg gctgccgcgc
     1621 gggttctttt tcgtctaggc tgcgggcatc ggttgtccca ccaggagtca atggtcatgt
     1681 catcgagctg gatcgttatc ggcgtaatcg tcattctcgt gcttttcgcg ttcgcggcct
     1741 acaatcgcct cgtcgcgctc agtcagcggg tcgggcaatc ctttgccgat atcgacgtgc
     1801 agctcaagca gcgccacgat ttgatcccaa acctgctcga gaccgtgaag ggctacgccg
     1861 cgcacgagcg cggcacgctc gacgacgtcg taaaagcccg caatgcggcg atgtcggcgc
     1921 agggaccggg acaggtcgcc gccgccgaaa accagttgag cggcgcgctc ggcaagctga
     1981 tcgcgctgtc cgaggcctat ccggatctga aagccaacgc caacttccag caactggcga
     2041 gcgagttatc cgatctggag aacaagatcg ccgccagccg ccgcttcttc aacaacgcgg
     2101 ttcaggaata caataccggc atccagcaga tgcccgcagc acttttcgcc ggcatgttcg
     2161 gcttcacccg caaggatttc ttcgacctcg gggctaaccg agccgaagtc gaggcggtgc
     2221 cgacggtgaa attctgagcc tgaaaaatat cccggcagag tagcccatca tggccgccta
     2281 tggtctttac acgcatatcg catcgaacaa gtttcgttcg atgctgctgc tcgccgggct
     2341 tttcctgctg atctacgtgc tggtctatgc cggcgcgctg gtcgcggagg tggtgatcga
     2401 cagcggcgag tcggccgaca cctatctggc ggcggcaggg cgcgacctgg tgacggcttt
     2461 tccctatgcg accggagccg ccgtgctgtg gatcgtgatc gcctatttct tccatcagtc
     2521 gatcgtcgac gccgttaccg gcggcgagga cgtgacgcgg cagcagcagc cccggctcta
     2581 caacctcttg gaaaatctct gcatcttgcg cggcatcccg atgccgaaac tcaaggtgat
     2641 ggatagcgag gcattgaacg cattcgcgac cgggctcaac cagcggcaat acgccgtcac
     2701 cgtcacctcg ggcctcctga aggcgctcaa cgatcaggag atcgaggcgg tgctgggcca
     2761 cga
//
LOCUS       DASWGM010000789        42110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2466090,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000789 DASWGM010000000
VERSION     DASWGM010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 42110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42110
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466090"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(909..1748)
                     /locus_tag="VGA15_21720"
     CDS             complement(909..1748)
                     /locus_tag="VGA15_21720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX9590349.1"
                     /translation="MKRVVGLREIADQFDLFLVDQYGVLHDGVGAYPGAIDGLARIKS
                     RGRKAVVLTNSGKDAAANLARLSALGFAESGFDAVVSSGDVALQCIKKGLLGPRFAQG
                     ANVCVIGRRGDTYAFSSDGFRLVDRPRDAAFLVFAGSDAPRTSLDSYRSMLTAAAQAH
                     VPAICINPDITMIRDGELVPAPGAIAKVYEDLGGKVEYVGKPYNAIFDHAVATAAVGP
                     RARVVMIGDSPEHDVAGAKAMGLSTLLVQTGIHRHLPEQQLLGFCEECGGTPDFLMAA
                     FEW"
     gene            complement(1745..2770)
                     /locus_tag="VGA15_21725"
     CDS             complement(1745..2770)
                     /locus_tag="VGA15_21725"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX9590350.1"
                     /translation="MPVDELGRPVAAGSLYNDSADDAAVGRVASARAAVHGATSPLAR
                     AARLQRATRVTRIIHQADWIAGQFSGRFDISDESNALKTGYDPVTRAWPEWIEAAGVE
                     RGKLPAVVPAGTAIGEVTAEAACRFGVPARAVVVAGTTDGCAAFLAIGASKPGEAVTS
                     LGSTMVLKLASEQPLFAPEYGLYSHRIGGMWLAGGASNTGGAVLAQFFTPEAIRDISR
                     RIDPNVTTGLDYYPLPAAGERFPINDPALTPRITPRPADDAVFLHALLEGIANIEALG
                     YRRLAELGGPQLTSVATVGGGAANERWGHIRAAVLGVPLRMPESDEAAVGVARLALRA
                     IGVRSQL"
     gene            2964..4181
                     /locus_tag="VGA15_21730"
     CDS             2964..4181
                     /locus_tag="VGA15_21730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9590351.1"
                     /translation="MVAAAGAAAETTVKWLHIEANPAQVKIWEEVARAYEATHPGVKI
                     EMQFLENEAYKAKLPTILQSKDRPHIIYSWAGGVLKSQIEAGVLEDITDQVKGYSDTI
                     TPAALAVFSQNGHVYGLPIALSQVGFLYNKDLMAKAKVDATTIKTWDDLLAAVKALKA
                     AGVTPIVVGGADKWPLHFYWTHLAVRIGGKAAFDAALRGENGGFAGATFQQSGELFKQ
                     LVDLQPFQNGFLGFKNPQAVGYFGDGKAAMTLAISTTYHLQRALAADKVGLGEDKIGW
                     FDFPVVPGGKGEPSDTLGGITGWLITKGAPKEAVDFLRFFISKDVQTRLAAGNFLIPV
                     VKGADSGLNNPFMKRIAENLAKSNYHQNFYDQSLGPSVGRVVNDAAAEIAGGSMTPQQ
                     AATAIEAAWKQGN"
     gene            4235..5125
                     /locus_tag="VGA15_21735"
     CDS             4235..5125
                     /locus_tag="VGA15_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9590352.1"
                     /translation="MGPLPVRHTIRDGRFAVLVLFLPPALVLFTLFVVLPIGEAAWYS
                     GFNWNGFGKPTNWIGLDNYRFVLETRAFWLALRNNGLIIAVSLLIQLPLALAIALMLA
                     ERFRGAIGLRMLFFLPYILAEIATGLIFSFVYDGDYGLVASIWHAFGAEAPYLLASTD
                     TAMLAVLIVIVWKYFGFHMMLFIAALQGMDKNLVEAARIDGATRPQVLRYVVIPLLYP
                     TIRLSVFFAIVGSLQLFDLVMPLTRGGPADSSNTMVSFLYNNGISRMRVGYGSAIGVI
                     LFVICVTFAFTYKRWFMRDE"
     gene            5118..5966
                     /locus_tag="VGA15_21740"
     CDS             5118..5966
                     /locus_tag="VGA15_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9590353.1"
                     /translation="MSNMETTRPAVDLAVLYKALFLALVATFVLVPLLATVLGGFKSL
                     GELRVNPFGLPHHWEWSNYADILLSKRYWQLLCNSMIISTLTVTLTLIVASMAAFIFA
                     HVKFFGSSMLLSYLTLGLLFPAATAVLPLFIKVRDLGLLDTYFGVALPQVAFSLAMSI
                     LLLRRFFKDLPHELLEAALVDGCSYINFFRYVTLPLSRPILATVGTITFVNSWNAYLL
                     PLVMLNTDDLYPWPLGIMVYQGEYSSEWHLILAFITLTILPTIILFFFAQKHIVAGLT
                     AGAVKG"
     gene            6009..7106
                     /locus_tag="VGA15_21745"
     CDS             6009..7106
                     /locus_tag="VGA15_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9590354.1"
                     /translation="MRRVGIGIIGCGNISAAYLKAAQRFPILDIKAVADMRSEAAEKR
                     GAEFRLPAMRVDQLLKRDDIEIVINLTVPLAHTDVSLAVLNAGKHVHSEKPLGVNVAE
                     ARKVMDLAAQKDFRVGCAPDTFLGGGHQTARKLIDDGAIGTPVAGSAFFMCPGHERWH
                     PAPGFYYLRGGGPMLDMGPYYITDLVQLLGPVASVMGSTARPKSERLVTSEPMKGTLI
                     PVEVATHVAGTLEFESGAVVSIAMSFDVPKHRHTPIEIYGDKGSMLVPDPNRFGGEVQ
                     VAKTGGEWESVLLTHGHVDGEFRSIGVADMATAIVTNRPHRASGALAFHVLEVIEAFQ
                     TSSDEGRRVKIGSRVDRPAMMPAGRETGQID"
     gene            7119..7760
                     /locus_tag="VGA15_21750"
     CDS             7119..7760
                     /locus_tag="VGA15_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEX9590355.1"
                     /translation="MRKAMIVWGGWPGHDPDLCASLIRGWLKQEGFDVRIETTTAAFA
                     DPAIHDLSLIIPIYTMSKIEKPEALNLCAAVRSGVGLAGHHGGMCDAFRDSVEYQFLC
                     GGQWVAHPGNIIDCKVDLMKPDDPIMQGLKSFEHRSEQYYMHVDPANEVLATTTFTGE
                     HAPWIEGVVMPVVWKKRYGEGRVFYSSLGHRAYELDVPEIRTIMTRGMLWAAR"
     gene            7760..8809
                     /locus_tag="VGA15_21755"
     CDS             7760..8809
                     /locus_tag="VGA15_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9590356.1"
                     /translation="MASGETNPAGRARLEDVARAAGVSLATVDRVVNRREGVREKTIA
                     RVEAAVARLGYRADPAAARLARNQSFRFAFILPTGSNSFMANLTEQVQRSADWLASQR
                     AFIDVIQVDVFDPDALAGALEQLSPAYHGVATVALDHPRVRAAIDDLVVRNVAVVTLV
                     SDAPSSRRMHYVGIDNPAAGRTAATLMGRFLSGRKGTVGVIAGSLSLRDHAERQFGFH
                     QILSSEYPDLTALPALQGRDDSECTRMLTAELLKRHPDLLGIYSAGAGNRGIAAALEE
                     AGKARDIVWIAHELTQHTRRFLLHGTIDAIINQDPGHEARSAARILLAHCWAEPIVLD
                     QERIRIDIFLRDNLP"
     gene            complement(9331..11040)
                     /locus_tag="VGA15_21760"
     CDS             complement(9331..11040)
                     /locus_tag="VGA15_21760"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX9590357.1"
                     /translation="MDFNPLLGHLIYMSLTLIIVAAIAAIWLAERSVEHLKLAIAALC
                     FSAAVLLFVVTDFERAILLSSILAAAIFGASRFKYNHSGLKLIVTDLPLAFAGTVPFF
                     IVQYPLAVIAALAGGIALILAAVAALLYVRGPPVSLELQVLLFSIACISLVAAYRAGG
                     GAVSFQRIAALRRCFFSTFIASLLNPLSWRQFGGLTLSDIAKHPLPLMAAVPARSLDY
                     PDIIVIQHESIFDPRIFGLPVEPIVEAFLSPKNGLYGSLNVDIFGGGSWQSEFSLLTG
                     LSSASFGSNAYFLFKRGVGRFHNSLPKTLTALGYRTMLTSSCRRSFLNYDEFYRSIGI
                     NERIFTDNFHPPFDVNRFETTNSDALFLEAALGAHMKRIAGDAAPRFLYALTNFNHGP
                     HNRRLVAPGHFDSERAFAAASLPDAYYAEYYARLVETAVTWNRLKSELSSRFPRRSTL
                     IMHYGDHQPVMTRRIEGKLKLHIDARRQFRTFYAIETLNDCSDRLPSGRGQDLDIAFL
                     GTVALQQAGLPLDEIFATRASLLEHCGGAYFASSSERKRRFHRTLVDLGMIDVAPTVR
                     YQH"
     gene            complement(11379..11759)
                     /locus_tag="VGA15_21765"
     CDS             complement(11379..11759)
                     /locus_tag="VGA15_21765"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX9590358.1"
                     /translation="MNPTQFMPQYNRETLSGFQIAYEYIGELAGRRTKARDITPRING
                     YWENQSFHNYADYAMDSSFHSGFAKLLELSRAQRCAVMCAEALWWRCHRRIIADYLLV
                     AGETYTQARYRQSGEPYAGSDLAS"
     gene            12042..13322
                     /locus_tag="VGA15_21770"
     CDS             12042..13322
                     /locus_tag="VGA15_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9590359.1"
                     /translation="MPITTRRRLLVGSAAALALPAFARAQSEAKPRITAISQWSAGSD
                     GAAISALGKLFEERGGVWQHNPVPGFTTEMMNKLRAQIMAGDPPAISQLKGPEIAAWS
                     RIAPTVDISPWVKTAGFADRVPAELAKSCQPAGHWIALPLQVYRINTLFLSTKGMQRA
                     GVTKAPTSWAEFNDTAEKMKATGMIPVANGGIRWDDGMKFEIALAGISPEVYRRAIMQ
                     LDTDALKGKEVLASFEQLRKLANWMNPNIAAQGWAVNLPAFVKGDMGMVLMGGWAQGN
                     LMRAGATDADFSSGPAPQESGPPCFDLNADCFIFWKPKGADLDAGQKLFAEIVMEPST
                     QEMYSKITGSIPARTDVNLAAPGFTDGQRNSAQNLRDAIAKDRVLLSLGHNMAQSNGM
                     TAAMIDVLTEYVHNSAIPAVEGQKRLVAAVASAQ"
     gene            13333..14277
                     /locus_tag="VGA15_21775"
     CDS             13333..14277
                     /locus_tag="VGA15_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX9590360.1"
                     /translation="MRTGLTLPAAKQDAGREQGVWRRRFGGVLTELAIWLPLSIAAGH
                     VVVFTAWTTWVSFTASSLMPDYTWVGLRNYWAVTRTANFQIAYVNLVIFGFGFVLLTM
                     ALGLLLAILLDQRVRGENVLRTIFLYPLAVSFVVTGTVWSWLLNPGLGLQKLVQNLGW
                     LDFRFDWLVQSDKAIYTLILAGVWQGAGFAMALFLAGLRSVDADLVKAAQIDGAGPWR
                     MYRRVLLPTIWPIFITVFVILLRSAITSFDLVRALTGGGPGISTMLPTLVVYDFMFQR
                     SELGRGSAAAVLMLLSLVVVMAPYGLYVRWQRQRQAQA"
     gene            14274..15116
                     /locus_tag="VGA15_21780"
     CDS             14274..15116
                     /locus_tag="VGA15_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9590361.1"
                     /translation="MKWTRVIVYAALIILALLFLVPLGVVVVNSLRSSREIAATSLIG
                     WPRHLIFSNYGQAWNKFCMAEHCWGIEPYVINSLTLAIPATVLSTLLGALAGYSISLW
                     RFRGDQFVFGLVTLGIFLPEQMKLIPWVVVLRDLSLMNTIAGLVLIHTVQGMSFTTLF
                     CRNYYLGIPQDLLKAARIDGAGFFRIFWRIILPLSPPILIVTVIFQFTGIWNEFLYGV
                     TFTSGGQQPVTAALIALSAAITSEPMYGEQSAAVLIAALPTLLVYLFGGRYFLRGLTA
                     GAVK"
     gene            15127..16242
                     /locus_tag="VGA15_21785"
     CDS             15127..16242
                     /locus_tag="VGA15_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9590362.1"
                     /translation="MAALSIRGLSKRYANLEVLKGIDLDIESGEFTVLVGPSGCGKST
                     LLNIVAGLDRPSAGIVEIGGRVVNDIPPKDRDIAMVFQSYALYPSMTVRQNITFGMEC
                     RHVPRAQQETAVANVARLLQIEPLLDRRPSQLSGGQRQRVAMGRALVRDPLLFLFDEP
                     LSNLDAKLRVEMRMEIKRLHQRIGATIVYVTHDQIEAMTMATRIAVMHQGAVQQFADP
                     DTVYRYPANLFVARFMGSPPMNTMPARLVAEEGGPVVVIGAGRPDEVRLRLQGYDAAA
                     AFRGSEVVLGIRPECIAEGSRGFSGASGAPIVVNAPVEMIEPTGAETIVLLRLGGEPV
                     LARITPDIRPAPGGLAAFALDTRRICLFDPVTEGLIA"
     gene            16239..17006
                     /locus_tag="VGA15_21790"
     CDS             16239..17006
                     /locus_tag="VGA15_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590363.1"
                     /translation="MTQTSYAGLPGRVVLITGGASGIGAAFVRAFAAQQARVAFLDID
                     ADAGEALVREVASECDATPLFVPCDLLDIAALRAAMARVRAALGDAAVLVNNAANDQR
                     QVLSEVTPAEFDWMIGVNLRHVFFAAQAVVPQMQARGGGSIINMSSIAWMRGVPALPA
                     YAAAKAAIVGFTNSLARLVGPDRIRVNAIAPGMVITERQRRLWFPDEQKIAEVRARQA
                     IPDAVTPDDIARLALFLASDESQRITRQCFQVDGGLG"
     gene            17873..20038
                     /locus_tag="VGA15_21795"
     CDS             17873..20038
                     /locus_tag="VGA15_21795"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9590364.1"
                     /translation="MFKNLSIRLRILALAGLLILLSIVIAGAGWYAISQVSAALAESI
                     RVARQAQFVTVGIREFAGADRRVVSYLQTATDDDEQRYTTRKTDSDAAFGQALELVRD
                     PARRQAMLDGRQSLKDYFATADAIIRDRKALRQRVTSIQEALAALPGSAKKERDSAQS
                     PVQRLASMDSLRRAAQIGPKSSPIRPSSATAAGRSPRESDVGLVAAASGTGLGLADAG
                     PVSDTAKPDVAGGAAGRHAGLLQHIGEAAQLVALDPSDATLANYAAAIQAFGDTPPPN
                     DAAARLLLVAGDATAAARSILQNYDKLQETEPAFMDKLKALRLELTAFAGEIANGAIA
                     TAASGTSEVIYASAFAVVAGSLLAWFIVRGIIVPLNAMTGAMTGLAAGDGTVDIPEMR
                     SKSELGAMARAVDVFKQNADKIAAMLTAETTTREIGEVISGAAAGDLTIRVPLDNKVG
                     FLKDIGEQVNRLLNATNTAFKEFGEKARHTAISVGEASAAVGQVSDGARSQSAALSQV
                     ATALRESTDALKSVSDNTKAASDKASTASQLVQKGLTSVERLADIVEAIAQNSRKVNQ
                     ITQVIAQIANRTHILSLNAAIEAARAGEHGKGFVVVAQEVGKLAESAGQNAKQITDIV
                     EQATIDSNEGKSATDVVRGAMQGIAAQTDQTTQMIHSSAAAVEEQQATITQIDSSVSQ
                     LRSIATSNSSAAEEITATMVQLSQLADETRGRLAQFKTA"
     gene            20072..20920
                     /locus_tag="VGA15_21800"
     CDS             20072..20920
                     /locus_tag="VGA15_21800"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX9590365.1"
                     /translation="MNLAEIEPDSMALRLAEVVNRRVGLRPADAVRKVALSLACVEDA
                     HRLAYVERMIAASPRDRDWIDFVERMLVHETYFFRHTAQLDLLRDEILPRLDAQRREA
                     GRPMLVVWIAGCSTGEEAWTMAFLAHARGAGVFGEAVPVSILATDLSETSLATARHGV
                     YDRLHGLDSFRAIPLWAAAYFGPGRGTNAWIVPEILRGSVRFQRHNVLDPPPVASADL
                     VLCRNMLIYFDEAANRRAQENLVSALRPGGVLVLGPADAPRTMGGLEAIGTHSATVYL
                     KTAEVA"
     gene            20917..21978
                     /locus_tag="VGA15_21805"
     CDS             20917..21978
                     /locus_tag="VGA15_21805"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX9590366.1"
                     /translation="MSRVLVASPSAFLRIGLLQQLRRRQGNQGNIVPAEAATAADAVR
                     ILAGGGIDLAIVDDALMVTPAGDRLVAAIERHHGRTLVIATREPQGTQPASSGTLRVI
                     SGVVDGTLDIGAIARSLPMALDEVAVARGIRRAPAGAPLVPIAEPCARAPRGRPDMIL
                     VAASTGGPSALTLLLRHLGPPRLPVVIAQHMPADQTAGFAQYLAAESGVDVIEQGAGD
                     VPSQAHVIVLRGGSDYRLARGRAGQFRLVATEVAGNVFHPSADLLFGSAAEAGMTVIG
                     VVLSGMGDDGARGAAAIAAQGGRVLVQDFASCVVAGMPSATRAVCPSAAVASVPEIAE
                     RLSRWSMARIHRAEVVPEG"
     gene            21979..22377
                     /locus_tag="VGA15_21810"
     CDS             21979..22377
                     /locus_tag="VGA15_21810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590367.1"
                     /translation="MASLLLVTEDNGFATAAEALSNADLTVTRAHAEEAVAVAKHDLP
                     DVLAIDTDSIVEAKPLIATLSLVTRSIVVAVARQAWPGSEAADLWRDAGADVVLPKPS
                     GAASPSLAGADREAYAQWFADLVRTGENAP"
     gene            22374..25103
                     /locus_tag="VGA15_21815"
     CDS             22374..25103
                     /locus_tag="VGA15_21815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9590368.1"
                     /translation="MSNGADSQLMAQFNVEVDQHLAAIEPVLGMAEPDALTRSDIDLL
                     FREFHSIKGLARVVAATGMERLTHEAESLLSPVRSGDRAFDTTIQEPLIAATDALRDA
                     LAEPLGWRAPIKIIEALRAATAAIGPDGSSENTSDDAGNEPWRFLGDDPDLLRAFAEL
                     LDEVLPAAAQAIVNRDEDGLREAIDAVTFACTRLGFDGVGFVAQCVAVQTGAERVCAF
                     TRLMRKAARFGALVGEDCGVTGAAVVLQDLLRATLTTCLDEALAVQPQARRALLRRCV
                     PLCDVAAPNSGAGRLIEDIVEDLPGSVGHPRDREALLLEGIAALRRGLGSERDADLPV
                     ATFRDAICAPYRASEEIEAHLRRLGLDTRLFSLGPPRRLERLSTLLDGSGATLKEVFV
                     EAVTPASLASLARLDPLLGQSAMRGGRPGIAVLLLDADPPGAVAETVADGVGELRRLD
                     PGHVPGEFSVSSGSATGGPERAGETQVRVPVAVLDKLFGRVGEFFSIGSRLNVLATET
                     DVPDALRRLSDFAVTRAPQLQADIETLARQHRDFSTVEADVGRILSLIHESTLGLRVI
                     PLEMLTGRFPRMVRDAARAHGKQVRFLADTGGIRIDKGMSDMLADPLTHMLTNAVEHG
                     VEPEAERLAQGKPRAANLRLIAQQQGNRIVVEISDDGRGIDIERVKRRAMSSRLASED
                     EIRRMSDEQLARFIFTPGFSTREEITDASGRGVGMDVVLVHVTKLGGKIDITTQPGRG
                     TTFRLDLPLSAAIQPMLLADTGVQSIGFPEAMVSEALMFPSAGVQFVNGQRAILLRER
                     FLPVFRLTELLRLPPSTAANAAYLPIVLCQWAGQRMGVEVHRILRRGEMLIREAHPRV
                     AALPGIGGITSMGFDRIVLVLDPEGMFELAREASVSGLQVPAERLALEQGSAA"
     gene            25100..25579
                     /locus_tag="VGA15_21820"
     CDS             25100..25579
                     /locus_tag="VGA15_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590369.1"
                     /translation="MSEFLEVRCGMHRFLVPSADINSIEVLKGVPEPFGGRRAPREWL
                     LLDGRVLAGDDAGEATEHGVALRATEGTPLETRIVVDQVGALVHCDPSAIEPMPHAVA
                     RLRDLFSGIWREEESQEYLFCVRPRDELPVQRFVWRRRIRRAVMTIRPATGDRKAST"
     gene            25576..27294
                     /locus_tag="VGA15_21825"
     CDS             25576..27294
                     /locus_tag="VGA15_21825"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9590370.1"
                     /translation="MTLRAAPAAYTAARKRVLVVDDSFIMRNLVKEIVESDPDLEVVD
                     TAENGMVALQKVRQIKPDVVLLDIEMPEMSGLETLRRLGLRSPCKVVILSSLVGNTAS
                     AERIEALRLGAVATIAKPSGGVSLDLKQKRASQIVGVLRKTLGLPTIVDATPAEIEDQ
                     AGPSMRLTDLSPGPIGDQLLEGLETGVLVFGPDGSLVRANPAAGRILYGCDVNPNRTT
                     IGGFCDEFNHALGQEIWDVIAGGEALAPADVNIATPDGDWIAVRRAIRPIFVSGKPCG
                     ALVLLDDIAEKRRMQSLLEKTMSSDVARAMIDTPAAELGGAMRDATILFADIRGFTPL
                     AEALGARGVLDLLNEYFSYMADVIGAEGGIIDKFIGDAVMALFGVPTSHGDDADRAVA
                     AARNMHRALTLINERRAGPALRIGVGLGSGSVIAGQVGSPNRMNYTVIGDPANLASRI
                     EGVTKAYGCGILICDETFKRLTCPVPARKVDVIILNGRETPTAIYEVFVEDPGAAATE
                     WLGEFGAGVSAYVAGDFVAAQAHLARAMAINPDDTTAGVLAGRCRRLGLRPAGEWTGA
                     WKLTEK"
     gene            27304..28728
                     /locus_tag="VGA15_21830"
     CDS             27304..28728
                     /locus_tag="VGA15_21830"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX9590371.1"
                     /translation="MERLDFLCILAGARRVAIPLNLVLSVEEARPLTPLPFSPELVEG
                     LVMALGRVVPQMSLAAVLGEEKLEGGVLVVVAASDDIRALRVDQVAGMVQIDIQAVKT
                     TDEAERSAQSLLTARFEALGAEWHVLDYSGLTFDEQMHPAEGADGAALVAAAGEDADD
                     TPPPVEDVDQHLPLLVVEIAGESYALPTSDIVELLIPGVVRSMPAAPAWVAGMIDRRG
                     APLLVLSTAMLLGRPSTFKSTITLVVNIAGAGEVGMMIDRAVGIERVHRSAIHIVPRD
                     MAGVANYFVIETEHIVGIIDLVALTGQVGDAVAELAPREPDLPTPAQIVVESAKASHK
                     LLAVRLGRELFALPLDRVERIQASIVLTPLPQPGTGFHGMADVGDTMVPVLDLRRAIE
                     HAGSDPNPPCTLVQIEGALAGLVVDQVLRIEDIPDNDVEDITMNPRLPVSHVARSGDQ
                     LLSVLVLDRVLPPLGSILVAAAKP"
     gene            complement(28849..29328)
                     /locus_tag="VGA15_21835"
     CDS             complement(28849..29328)
                     /locus_tag="VGA15_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX9590372.1"
                     /translation="MLAHQPTWTVERIELLKNRFEAGLSCREIACDIGVSRNAVIGKL
                     SRLNLTREKNGEARRAARKDTPKAPRGRAVPRLQYQMLRALYAEPLPADDDEPIHNGH
                     TCSLLELSAEKCRWPISTPGAEDFCFCGNAPVEGLPYCPGHTRLAYRPGSRQRVARG"
     gene            complement(29410..29610)
                     /locus_tag="VGA15_21840"
     CDS             complement(29410..29610)
                     /locus_tag="VGA15_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590373.1"
                     /translation="MATEKAETDRVPVTLALSTISYLEKLVRQGTHGTSVPGVAKTLI
                     EEGIRLAIKDGLLAIRDNGKTQ"
     gene            29926..30834
                     /locus_tag="VGA15_21845"
     CDS             29926..30834
                     /locus_tag="VGA15_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590374.1"
                     /translation="MAKHDTFSAAASAAGYYYQARLALAEALRFAYAESGVEVAIEKL
                     DDVSFESAGSPLELLQSKHHIAKPADLTDYSADVWKTLRVWSSRVKQDPSLPSRTRFA
                     LVTTSAASSGSAAALLRPSTPTSDRNVDKATKLLVAVAKESSNAVLKSSFDEFLSLTS
                     EMQRSLLDAVDVLDASPNIVDLESIIEERLKMIAPRGKTKLAREHLEGWWWPRICKAL
                     LEPGVGKVSILELEARLDDIREIMKRDALPVEMDNVEVDDQHLEALDELTFVRQLRLN
                     QAWSSTHRTCKARFLPCIHATIPLDP"
     gene            30773..31126
                     /locus_tag="VGA15_21850"
     CDS             30773..31126
                     /locus_tag="VGA15_21850"
                     /inference="COORDINATES: protein motif:HMM:NF042658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HEX9590375.1"
                     /translation="MELAKRDFYRASTQRSRWTRENLLFDGEVSAFERTLVEEWQPRF
                     HAMCDTLAKNCAAAALRKAGQSLYQWVETGARFSFRTVSHRFLSVGSFHMLSNDVRVG
                     WHRDFLKTFNPSKEK"
     gene            31062..31646
                     /locus_tag="VGA15_21855"
     CDS             31062..31646
                     /locus_tag="VGA15_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007539562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three component ABC system middle component"
                     /protein_id="HEX9590376.1"
                     /translation="MTFELDGIATFSKLSTHPKRSEMALAKRSWGLAWGDRPREEATN
                     LNPAFCGELIFRSIAEFRRLRELPFPFALSFVVLPLVLHKRTRDQLPGTASTAFVGWV
                     AEHGPLLAELPQRALHLAPITREAIMFAMQHELLAVEQGGLIPGSKPIASRKRPERTT
                     DDADDARRSAGLIGRWFANQGLSSSIMQGLGVTP"
     gene            31643..33589
                     /locus_tag="VGA15_21860"
     CDS             31643..33589
                     /locus_tag="VGA15_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007539565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3732 domain-containing protein"
                     /protein_id="HEX9590377.1"
                     /translation="MTLQLRSISIYSHDEQRRDIVFKLGALNIVTGASKTGKSALLDI
                     VDYCWGRSDCTVPEGEIRRKVSWFAIHLDNAGEGILLARKNPGPAGRTSDEIYLERGV
                     EVLPVTTTSFQKNITGDGLRSQLSVVLGISENLHVPPDTASRQPMEASSRHAILFCLQ
                     AQDEIANRKFLFHRQGEQFFPAAIKDSLPYFLGAVDEAHFLALMRYNDARTRLRRAER
                     DQLEYRSAVDAASNTALSLVDEARRVGLISRVGGSDAASTHVLLTEAAMPQPMNLDLI
                     DDPTADMEQLEERRRRLRGDLQEVRDDIAEVERANRDASEFETETKEQEARLLSLELI
                     RGHESFPDHCPLCDSQLTVAVPTVDEIRQSLASIGTQLTSVRRDSPRLQERLALLEAR
                     RAELEELLRVVQRQIAQRIADNERLRIQQNQFSEQARVAGRIAYYLESYTSIGPTSDL
                     SQTIDRLKAEIAELEKVLDDDALETRIDTALSLVAQELTAYANSLSLEHGANPLRLDR
                     KHLTVVADTVDGPLSLAQMGSGENWVGYHVAAHLGLHKLLRRRSRPVPAFLMLDQPSQ
                     AHYPPEHDANGLIAGLADEDQAAVHRLFKLLSAYCVELTPRMQVIVSDHVELLDDWFR
                     DAIVQRWRDGIKLVPVDWLTAING"
     gene            33592..34674
                     /locus_tag="VGA15_21865"
     CDS             33592..34674
                     /locus_tag="VGA15_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590378.1"
                     /translation="MARDPITWDSQTGHYIPPKLTWQVDALETVGNVDAPEVWLSKPG
                     GEYRLAASFHFPGFNFPSGAGLLPSGIAYSPSSFVLHHAQDPYNSWNVKASTKRFSGP
                     MEHWDWLPGIMLVDERTGYSLPSNELSAVAGTEWADNDARIFHGLYDDAKYGEHLDVF
                     LQAVNAITGPGWANWPFGHRQLEHAVMCWLRPGVALPDVVLLLSVRQSGAKFSYRRKG
                     LYDPGERLGAWLQSPPKWDCRQAMRFLYQANLLAKDGLPELAVVSAIAALENASAEIL
                     LYLTGGNAAVVQSAFGKCKFLSRFDKVLPAYGPKLPSAEFSAVKAAYLARNGVAHALA
                     PVKQEDAIRHVKAVEDVLAWYIANGG"
     gene            34783..36123
                     /locus_tag="VGA15_21870"
     CDS             34783..36123
                     /locus_tag="VGA15_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX9590379.1"
                     /translation="MPIAVKQNMYPDSKSRSQKLYDRALTSLPGGNSRTTVFMKPYPI
                     YAARGEGCRVWDVDGNEYIDCVNNFTSLIHGHAHPLLVEAATRQLALGSAFGLPTLSE
                     IELAELLVSRLPSVEQVRFTNSGTEAVMMALKAARAFTGRPKIAKCEGAYHGSYDYAE
                     VSLDPTPEAWGRNAPVSVAYARGTPDNVLADVVTIPFNDAEAAVSLIREHGSELACVL
                     VDPMPNRAGLAPADRAYLEALRQITREVGALLIFDEVITFRLAYGGAQGLWNIDPDLT
                     TLGKIIGGGFPVGAIGGHKEFMAVFDPRSGKPALPHGGTFSANPVTMRAGLAAMELLD
                     HAAFVRLDMIGAAVRAGINEAFRRYGVPGGTVGLGSLLKIHFADRPIRDYRSAYMTEQ
                     EVLRQAIFNRGLLNRGVLAAGYGLMALSTPMTDADIDTVITAASDALEEVSASA"
     gene            36171..37844
                     /locus_tag="VGA15_21875"
     CDS             36171..37844
                     /locus_tag="VGA15_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX9590380.1"
                     /translation="MSPKIDPITRSVVQHRLSSIVKEMGEAMLRTSYSQILNSSRDFS
                     LGICDTQGRLIAQADHIPVHVGALPWATLAVEARFNDVRPGDVILLNDPYHGGSHLPD
                     LTAFVPIFDGERRLLWTIVRAHQSDIGGATHGGYNPGATEIFQEGLRVPPIKLYESGR
                     LRDDLLDLLALNIRNPREFRGDLAAMVGAAHLGERRLSRLFAEFGAPVVEAAVETILD
                     AAEAQTRAVVSTWKDGVFYGEAFLDDDGHGRSDIRIAARVTKSGSDIEVDLSDSDPQS
                     TSFVNSSHANMQAAVVMAFAYLIDADIPKNAGALRPLKVIAKQGTIVWADPGRPVTLC
                     TSHPSNEIVEAIVKAMSASCPDRAMAGWGRRFRIAIQGEDPRSGRNFIWHLFQARPGG
                     GASSGGDGWSSIGEWHSVGGIKFGSIEVAEVRFPLHFRHHEFRPGSGGDGQFRGGFGV
                     ALDLVLETAKPALANTAGDGARHGPCGMLGGEDGVPHDYRLISEGRAPRVLRTKEVGI
                     EIRPNDCLEVRSSGGGGWGPPARRSPAARQRDREQGLTQDSATMGQGGR"
     gene            37844..39886
                     /locus_tag="VGA15_21880"
     CDS             37844..39886
                     /locus_tag="VGA15_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX9590381.1"
                     /translation="MFTIGIDVGGTYTDLVAIDERGKAVFAKSPSTPSDQSVGVMAGL
                     EELARRLQITRAEMLGVTDRVVHGTTVATNALLERKGAHVALLTTEGHRDVIEMREGL
                     KGDRYDLRSPPPEPLVPRERRFGVKERLKANGEILTPLDDRSLGDAISAISASDATSV
                     AVCFLHSYLDPVHEIAAVERLARGLPGISISRSSDVLPQIKEYERVSTTIVNAYVGPL
                     VRHYLTNLEQRLKGAGFKGSLFIVLSHGGMAPVEEASRLAAGTVLSGPAGGVSGARRC
                     ADLLGISDLVPFDMGGTSTDISLISEGRASLSADGMLAGQRIALRSLDIASIAAGGGS
                     IASVDAGGTLRVGPESAGSVPGPACYGSGGLAATVTDANVVLGYLDAAAFMGGQRPLD
                     RAASEAAVDRVAVSLGLSWLEAAAGIYRMINLKMADGIRLMTLRRGVDPRRFALLSFG
                     GAAGLHAGEVARELDIKRIIVPTAASVLSAWGMLTSDLRYEVSRTHYGAGPRISADEA
                     RALFCRLEQQAAGRLRAWFDGPMSIERSAEMRYGEQIFEIDVALDDLDWDAASLVDQI
                     EDRFHRRHEELYTYSSRDQEVVFVNARVAAIGEVSRRGQDERPAPSAAACAPRTTRQA
                     FFGAWREVPVYALDALQPGHAFEGPAIVEAETTTVIVGAGDRVTVNALGWLDIALR"
     gene            complement(39951..40238)
                     /locus_tag="VGA15_21885"
     CDS             complement(39951..40238)
                     /locus_tag="VGA15_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEX9590382.1"
                     /translation="MAKKNPHVGSSFESWLEDAGIREEATAAAIKAVIARQLASEMKK
                     KKITKQRMAELMKTSRAQVDRLLDPDNGSATIESLQRAAKIVGRELRLQLV"
     gene            complement(40214..40585)
                     /locus_tag="VGA15_21890"
     CDS             complement(40214..40585)
                     /locus_tag="VGA15_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9590383.1"
                     /translation="MPESFKPIPLMFWRSATGREPVREWLNELSRDDKRTIGRDIAKV
                     QYGWPVGLPLCRALSGGLWEVRASLPSKREARIFFGFHDGILVALHAMIKKTQKTPTE
                     DLTLARQRFKELQLWQRKIRT"
     gene            40756..>42110
                     /gene="htpG"
                     /locus_tag="VGA15_21895"
     CDS             40756..>42110
                     /gene="htpG"
                     /locus_tag="VGA15_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX9590384.1"
                     /translation="MTATTDTAAPESQPFQAEVAELLHLMVHSVYSETDIFLRELISN
                     ASDACDKLRYEAIAAPDLIADGEPPKILIAPDKKADTFSVIDSGIGMDRQELIDNLGT
                     IARSGTRSFLSRLTEAKDGAGLIGQFGVGFYSAFMVADRIVVTSRRAGADQVWTWSSS
                     GGSGFEIAPASEDDAKRIMRGTEIVLHLKPDAKKYLEIYEIERVVGAYSDNIQFPILL
                     KSEDGEPRQINSASALWQRPKSELTPEDYKQAYKSIAGAFDEPAMTLHYRAEGRYSYA
                     VLLFAPSTKPFDLFEPSRKGRVKLYVRRVFITDDADLLPAYLRFIRGVVDSEDLPLNI
                     SREMLQNNPQLAQIRKAVTSRVITELEQLGEKEPENFAKIWDAFGPVIKEGLYEDYER
                     REKLLALSRFTTTAGEKRSLKQYVADLKPNQTEVYFLAGDSIERLKSNPKLEQAAARG
                     I"
BASE COUNT         7811 a        12863 c        13249 g         8187 t
ORIGIN      
        1 cgctgcttgc gcaatcaccc ggatgaggag cggggagaga cgtcacgcca acgggttttt
       61 catttcacca cgccgatttt ttgatcgcgc aggcgcgtcg cgggcggccg cctcagcttt
      121 tctaacccgc tcgcgcaggt cctgtacctc gcggtattct gttgcaagcg agtagagcaa
      181 tttggtcggc gcgttcgcga atgcgagatg gttccaagcg gcggaaagcg cacaggaaat
      241 ccgcgatccg aagtcttatc cctcctgtgc gctagcgttt ttgatttcac acgagattgc
      301 aaagtacctt gaacgactcg gctctcgcca cagttgcagt ccattgcttt caccgtgcgc
      361 gctcggcagt caatgcgatc tgcgtttcga cagtaagaat gattgcgtca atctgtttgg
      421 cgttcagcgt tcgatgttcg atcagtgcat tggaaaccgc caacactcca ccgaaaacgc
      481 gacaacgata ataatgcaat cggcggccga gatgccgtct gcgcgcgcca gaaatgcagg
      541 gatagctttc atggttcaag ctggcgccag aaccctttaa gcgaggatta attcagtgag
      601 ggaagccaat taaacaaaaa gaccgcggtc tctttggaac gagacgggtt ttcctaagtt
      661 gaggcggtat tcggcgagga gaatgcggtg tccaagaaat caatcatagc tgtggcgttg
      721 gacgcggctc tcattgcgcc actccacctt gcgcaagcgc ggcgcgcata tcgcgttcga
      781 gaaggcgccg cgccttcagg aaaagagcta gcgactcccg atcgcaaaat cggagagttt
      841 gtcgtacata aaggtcgcat gactgatttg cgcgatgctg ccgcccgcgg gcgccggccg
      901 atcgagggtc accattcgaa ggccgccata aggaaatccg gagtgccgcc acattcttcg
      961 cagaatccca acagctgctg ctcgggtaga tgacgatgaa tgccggtttg cacgagcagc
     1021 gttgacagcc ccatcgcctt ggcgccagcc acgtcatgtt ccggactgtc gccgatcatg
     1081 acgacgcggg cgcgtgggcc caccgcggcg gtcgcgacgg cgtggtcgaa gatagcattg
     1141 taaggcttac cgacatattc caccttacca ccgaggtctt cgtagacctt tgcaatggca
     1201 ccgggagcgg gaacaagctc gccgtcccgg atcatcgtga tgtccgggtt gatgcaaatc
     1261 gccggcacat gtgcctgtgc ggcggcggtc aacatcgagc ggtagctgtc cagcgaggtc
     1321 cgcggcgcgt ccgaaccggc aaagaccagg aaggcggcgt cccgcggccg atccaccagc
     1381 ctgaagccgt ccgatgaaaa ggcgtacgta tcaccgcggc gtccgatcac acagacattc
     1441 gcgccctgag caaatcgcgg gcctagtaag cccttcttga tgcattgcaa ggccacgtcg
     1501 ccggagctga cgacagcatc gaaacctgat tctgcaaaac cgagggcgct gagccgcgcc
     1561 aggtttgccg ccgcgtcctt cccggaattc gtcagcacga cggcctttcg cccgcgggac
     1621 ttaattctgg ccagtccgtc aatggcaccc ggataggctc cgacgccgtc atgcagcacc
     1681 ccgtattgat ccacgaggaa gaggtcaaac tgatccgcga tctcgcgcaa accgacgaca
     1741 cgtttcatag ctgggatcgc accccgatgg cgcgcagcgc gaggcgcgcg acgccgacgg
     1801 cagcttcgtc gctctcaggc attcgcaggg gcacgccgag cactgctgcg cggatatggc
     1861 cccaccgctc attcgcagcg ccgccaccga cggtcgcgac gctggtcagt tgtggaccgc
     1921 caagttcggc caggcgccgg tagccgagcg cttcaatgtt cgcgatgcct tccaacagtg
     1981 catgcagaaa caccgcatcg tcggctggcc gcggcgttat ccgcggcgtc aatgccggat
     2041 cgttgatggg gaagcgttcg cccgccgctg gaagagggta atagtcgagg ccggtggtaa
     2101 cgttgggatc aattcggcgg ctgatgtcgc ggatagcttc cggcgtgaag aactgcgcca
     2161 acacggcgcc acccgtgttc gaggcgccgc cggccagcca catacccccg atccgatggc
     2221 tgtaaagacc atattccgga gcaaacaatg gttgttcgct cgccagtttc aggaccattg
     2281 tcgaacctag tgacgtgacg gcttctcccg gcttgctcgc gccgatagca agaaaggccg
     2341 cgcatccgtc cgtcgtgcct gcgacaacga ccgctcgggc cggcacgcca aaacggcacg
     2401 cggcctcggc agtgacttct ccaatggctg taccagccgg aacgaccgcg ggcagcttgc
     2461 cgcgctcgac gcccgcggcc tcgatccatt ctggccaagc ccgggtgacc ggatcatacc
     2521 cggttttcag tgcgttgctt tcgtcactga tatcgaagcg tcctgaaaac tgaccagcga
     2581 tccagtcagc ctggtggata atccgcgtaa cgcgcgttgc ccgctgcaag cgcgcggcgc
     2641 gcgcgagcgg cgacgttgcg ccgtgaaccg cggcgcgcgc cgacgccacc ctgccgaccg
     2701 cagcgtcatc ggcggaatcg ttgtagagac ttcccgcagc gacggggcgt cccaattcgt
     2761 cgaccggcac aagcgtgccc gacgtgccgt ccaccgcgag ggctcagtcc ggttgatggt
     2821 tatagctcaa actttgagtt gcgcgcctca aaaaatgcgg tagtttcgcg gcaaccggcc
     2881 gcctgacgcc ggaaccgagg attctcgtgg gactgctgac agcaggagtg ctgcaaaggc
     2941 tggtgttggt tgccgccgcg ctgatggtgg ctgccgcggg cgccgcggcg gagaccaccg
     3001 tgaagtggct ccatatcgag gccaatccgg cccaggtcaa gatctgggaa gaggtcgccc
     3061 gcgcctatga agccacccat cccggcgtca agatcgagat gcagttcctc gagaacgagg
     3121 cgtacaaggc aaagctgccg accatcctgc aatccaagga ccggccgcac attatctata
     3181 gctgggcggg cggcgtcctg aagtcgcaga tcgaggccgg cgttctcgaa gacatcaccg
     3241 atcaggtgaa gggttacagc gacaccatca cgccggcggc actcgccgtc ttcagccaga
     3301 acggccacgt ctacggcctg cccatcgcgt tgtcgcaggt cggcttcctc tacaacaagg
     3361 acttgatggc gaaagccaag gtcgacgcga ccacaatcaa gacctgggac gatctgctgg
     3421 ccgcggtgaa ggcgctgaag gcggccggcg tcacgccgat cgtggtcggc ggcgccgaca
     3481 agtggccgct gcatttctac tggacccacc tcgcggtgcg gatcggcggc aaggcggcgt
     3541 tcgatgcggc gctgcgcggc gagaacggcg gcttcgccgg agcgacgttc cagcagtccg
     3601 gcgaactgtt caagcagctc gtcgacctgc agccgttcca gaacggcttc ctcggcttca
     3661 agaatccgca ggccgtcgga tatttcggcg acggcaaggc cgcgatgacg ctggccatca
     3721 gcacgaccta tcatctgcag cgcgcgctcg ccgccgacaa ggtcggcctc ggcgaggaca
     3781 agatcggctg gttcgatttt cccgtcgtgc ccggcggcaa gggcgagccg agtgatacgc
     3841 tcggcggcat caccggctgg ctcatcacca agggcgcgcc gaaggaggcc gtcgacttcc
     3901 tcaggttctt catctccaag gacgtgcaga cgcggctcgc cgccggcaat ttcctcattc
     3961 ccgtggtgaa gggtgcagac tcaggcctca acaacccctt catgaagcgg atcgccgaaa
     4021 acctggcgaa gtcgaactac catcagaact tctatgacca gagcctcggt ccgtcggtcg
     4081 gccgcgtcgt caacgacgcc gccgcggaaa tcgccggcgg cagcatgacg ccgcaacagg
     4141 cggcgaccgc gatcgaggcc gcgtggaagc agggcaactg acggtgcgcg gacaagcggc
     4201 aggcgcgccg cctctcgcct ccgtgagatc gtcgatgggg ccgctgccgg tccgccacac
     4261 cattcgcgac ggccggttcg cggtgctggt gctgttcctg ccgcccgcgc tcgtgctgtt
     4321 cacgttgttc gttgtgctgc cgatcggcga ggcggcctgg tattccggct tcaactggaa
     4381 cggtttcggc aagccgacca actggattgg cctcgacaat tatcgcttcg tgctggaaac
     4441 ccgcgccttc tggctcgcgc tgcgcaacaa cggcctgatc atcgccgtct cgctcctgat
     4501 ccagctgccg ctggcgttgg cgatcgcgct gatgctggcc gagcgttttc gcggcgcgat
     4561 cgggctgcgc atgctgttct tcctgcccta tatcctggcc gagatcgcga ccggcttgat
     4621 cttcagcttc gtctatgacg gcgactatgg cctcgtcgca tcgatttggc acgccttcgg
     4681 cgccgaggcg ccgtatctgc tcgcgagcac cgacaccgct atgctggcgg tcctgatcgt
     4741 catcgtctgg aagtacttcg gcttccacat gatgctgttc atcgcggcgc tgcagggcat
     4801 ggacaagaat ctcgtcgagg cggcgcgcat cgacggcgcg acgaggccgc aggtgctgcg
     4861 ctatgtggtc attccgctgc tctacccgac gatccggctc tcggtgttct tcgccatcgt
     4921 cggctcgctg caattgttcg acctggtgat gccgctgacg cgcggcggcc cggcggattc
     4981 gtcgaacacc atggtgagct tcctctacaa caacggcatc tcgcgcatgc gggtcggcta
     5041 cggcagcgcc atcggcgtga tcctgttcgt gatctgcgtt accttcgcct tcacctacaa
     5101 acgctggttc atgcgcgatg agtaacatgg agacgacccg ccctgccgtc gacctcgcgg
     5161 tgctctacaa ggcgctgttt ctcgcgctcg tcgccacctt cgtgctcgtg ccgctgctgg
     5221 cgaccgtgct cggcggtttc aagtcgctcg gcgaactgcg cgtcaatccg ttcgggctgc
     5281 cgcatcactg ggaatggtcg aactatgccg acatcctgct ctcgaagcgc tactggcagc
     5341 tgttgtgcaa ctcgatgatc atctcgacgc tgaccgtgac gctgacgctg atcgtcgcat
     5401 cgatggcggc gttcatcttt gcccatgtga aattcttcgg cagctccatg ctgctgagct
     5461 acctgacgct gggtctgttg tttccggccg cgaccgcggt gctgccgctg ttcatcaagg
     5521 tgcgcgacct cgggctgctc gacacctatt tcggggtggc gctgccgcag gtggcgttca
     5581 gtctcgcgat gagcatcctg ttgctgcggc gcttcttcaa ggatcttccg cacgaattgc
     5641 tggaagccgc gctggtcgac ggctgcagct acatcaactt cttccgctac gtgacgttgc
     5701 cgctgtcgcg gccgattctg gccaccgtcg gcaccatcac cttcgtcaac agctggaatg
     5761 cctatctgct gccgctggtg atgctgaaca ccgacgacct ctatccatgg ccgctcggca
     5821 tcatggtcta tcagggcgag tattcgtccg aatggcatct gatcctcgct ttcatcacgc
     5881 tgacgatcct gccaaccatc attctcttct tcttcgcgca aaagcacatc gtcgccggcc
     5941 tcacagcggg cgcggtcaag ggatgacagg tcgcgccgcg gcgcgcatca attactatgg
     6001 agaacgcaat gagaagagtc ggtatcggaa tcatcggctg cggcaatatc agcgccgcct
     6061 atctgaaggc ggcgcagcgc tttccgatcc tcgacatcaa ggccgtggcc gacatgcgca
     6121 gcgaggcggc ggagaagcga ggggccgaat ttcgcctgcc ggcgatgcgc gtcgaccagt
     6181 tgctcaagcg cgacgacatt gaaatcgtca tcaacctcac cgtgccgctc gcccacaccg
     6241 acgtcagcct cgccgtgctc aacgccggca agcatgtgca ttcggaaaag ccgctcggcg
     6301 tcaatgtcgc cgaggcgagg aaggtgatgg atctcgcggc gcaaaaggat ttccgcgtcg
     6361 gctgcgcgcc ggatacgttt ctcggcggcg gccatcagac cgcgcgcaag ctgatcgacg
     6421 acggcgcgat cggcacgccg gtcgctggca gcgccttctt catgtgcccg gggcacgaac
     6481 gatggcaccc tgcgccgggc ttctactatt tgcgtggtgg cggaccgatg ctcgacatgg
     6541 gtccgtatta catcaccgac ctcgtgcagc ttctgggtcc cgtcgcaagc gtgatgggct
     6601 cgacggcgcg accgaaatcg gagcggctgg tgacgagcga gccgatgaag ggcacgctca
     6661 ttcccgtcga ggtcgcgacc catgtcgccg gcacgctgga attcgagagc ggcgcggtgg
     6721 tctccatcgc gatgagcttc gacgtgccga agcaccggca taccccgatc gaaatctacg
     6781 gcgacaaggg cagcatgctg gtgcccgacc ccaaccgttt cggcggcgag gtgcaggtgg
     6841 ctaagaccgg tggggagtgg gaatcggtcc tgctcaccca tggtcatgtc gacggcgaat
     6901 tccgctcgat cggtgtcgct gacatggcga cggcaatcgt caccaaccgg ccgcatcgcg
     6961 ccagcggcgc gctggcgttc catgttctcg aagtcataga ggcgttccag acctcctccg
     7021 acgaaggccg gcgcgtgaag atcggaagcc gcgtcgacag gccggcgatg atgccggcgg
     7081 gacgcgaaac cggacagatt gattgaggga caacaacgat gcgcaaggcg atgatcgtat
     7141 ggggcggttg gccggggcat gatcccgatc tgtgcgcctc gctgatacgg ggctggctga
     7201 agcaggaggg attcgacgtg cggatcgaga ccacgacggc ggcatttgcc gatccggcga
     7261 tccatgacct gtcgttgatc attcccatct acaccatgtc gaagatcgag aagccggaag
     7321 ctctcaatct gtgtgcggcg gtgcggagcg gcgtcgggct ggccggccat catggtggca
     7381 tgtgcgatgc cttccgcgat tcggtggagt atcagtttct ctgcggcggc cagtgggtgg
     7441 cgcatccggg caacatcatc gactgcaagg tcgatttgat gaagccggat gatccgatca
     7501 tgcagggcct gaaaagcttc gagcaccgat ccgagcagta ttacatgcat gtcgaccccg
     7561 ccaacgaggt gttggcgacc acgaccttca ccggcgagca cgcgccctgg atcgagggcg
     7621 tcgtcatgcc ggtggtgtgg aagaagcgct atggcgaagg ccgggtgttt tactcctcgc
     7681 tcggccatcg cgcctatgaa ctcgacgtgc cggagatcag gacgatcatg acccgcggca
     7741 tgctgtgggc ggcgcgctga tggcgagcgg cgaaaccaat ccggccggac gcgcgaggct
     7801 cgaagacgtc gcgcgcgcgg ccggcgtttc gctggcgacc gtcgaccgcg tggtcaaccg
     7861 acgcgagggg gtacgcgaga agaccatcgc gcgcgtggaa gctgccgttg ccaggctcgg
     7921 ctaccgcgcc gatcccgcgg cggcgcggct tgcgcgcaac cagagcttcc gcttcgcgtt
     7981 catcctgccg accggcagca acagcttcat ggcgaacctg accgagcagg ttcagcgcag
     8041 cgccgattgg ctcgccagcc agcgggcctt catcgatgtc attcaggtcg acgtgttcga
     8101 tccggatgcg ctggcgggcg cgctggagca gttgtcgccg gcctatcacg gcgttgcgac
     8161 ggtcgcgctc gatcatccgc gcgtccgcgc cgcgatcgac gatctcgtcg tccgcaacgt
     8221 cgccgtggtg acgctggtgt cggacgcgcc gagctcgcgc cgcatgcact atgtcggcat
     8281 cgacaatccc gccgccggcc gcaccgcggc gacgctgatg ggccgcttcc tgtcgggacg
     8341 caagggcact gtgggcgtca tcgccggatc gttgtcgctg cgcgaccatg ccgagcgcca
     8401 attcggattc caccagatcc tgtcgagtga atatcccgat ctgactgcgc taccggcgct
     8461 tcaggggcgc gatgacagcg aatgcacgcg gatgctgacg gccgagctgc tgaaacggca
     8521 tcccgacctg ctcggcatct acagcgccgg cgccggcaac cggggcatcg ccgcggcgtt
     8581 ggaggaagcg ggcaaggccc gcgacatcgt ctggatcgcc catgaactga cccagcacac
     8641 ccgccgcttc ctgctgcatg gcacgatcga cgccatcatc aaccaggacc ccggccacga
     8701 ggcccgctcg gcggcgcgga tcctactcgc gcattgctgg gccgagccga tcgttctcga
     8761 tcaggaacgt atccgcatcg acatctttct ccgggataat ctgccttagc tcatgatgag
     8821 ttcaggttga accgtttccg tcattccaag agtctgactc aagatacagg caacagaatc
     8881 aggcgatgaa ctcactggca atctcggcga cgcgattgag aacacatcga tcggcgattg
     8941 tcgcttgttt cgtcctttag cggaaaattt ctcgctgcgc gttttgggac ttttgcaaca
     9001 ctatctgcca ccagcggacg gactcatgcg gagcagcagc attaattttc aaatcccttg
     9061 gatttaaact gggacgcagg ggttacagca tcccgccccc gataaatgaa atcgaagaag
     9121 gtggcactct tcgtagctac cggtcgtgtt cgtggacgtc tgcattgggt tgtttcggat
     9181 gtaggttctc gcgtcagcga ggtccgcttg cccaaattac cgtaaagctt tgctttgtga
     9241 gatacccgat aagccagtga gcgtccacgc ggctgggcca gatcgcccaa agcatattcc
     9301 caaggactcc actctcggac ccacaaatgt ttagtgttga tatcgcacag ttggcgccac
     9361 gtcgatcatg cctaaatcga caagtgtgcg gtggaagcgg cgtttccgtt ccgaggacga
     9421 tgcgaagtag gctccgccgc aatgctcgag caggctcgcg cgcgtggcaa agatctcatc
     9481 aagcggaaga ccagcctgct gcaaagccac tgttccaaga aaagcaatat ctaagtcttg
     9541 gccccgccca gaaggaagtc gatcagagca gtcattcaaa gtttctatag catagaacgt
     9601 gcggaactgg cgcctcgcgt ctatgtggag tttgagtttt ccctcgatcc gccgtgtcat
     9661 tacgggttga tgatctccgt agtgcataat cagtgtcgaa cgtctgggaa aacgggagga
     9721 aagctcggac ttgagcctat tccaggtcac ggcggtctct acgagtcgag cgtagtactc
     9781 agcataataa gcatcgggga ggcttgcggc agcaaaggct cgctcgctat caaagtgtcc
     9841 aggcgcaacc agccttcggt tgtgtgggcc gtgattgaag ttagttagcg catatagaaa
     9901 gcgaggtgcc gcatcgccag caatcctttt catatgagca ccgagtgcag cttccagaaa
     9961 caatgcatcg gaatttgtcg tttcgaatcg attgacatcg aagggcggat gaaaattatc
    10021 tgtgaaaata cgttcgttga taccaattga acggtaaaac tcatcgtagt tgagaaagct
    10081 gcgacggcag ctcgaagtga gcattgttct atacccaagc gcagttagcg tctttggaag
    10141 actgttgtgg aatcggccga cgcctcgctt aaaaagaaaa taggcgtttg aaccgaagct
    10201 cgcgcttgaa aggccagtta gtaggctaaa ttcagattgc cacgatcctc cgccaaaaat
    10261 atcgacgtta aggcttccat agagaccatt tttcggtgac agaaatgctt cgacaattgg
    10321 ctcaaccggg agcccaaata tgcgcgggtc gaagattgac tcgtgctgga tgacgataat
    10381 gtcgggatag tcgaggctgc gcgctggtac ggccgccatc agcggcaacg ggtgtttagc
    10441 tatatcgctc agagtcagcc cgccgaactg ccgccaggaa agtgggttga gaagggaggc
    10501 tatgaaggtc gaaaaaaaac accgccgcag agccgctatt cgctggaaag aaacggcccc
    10561 gccgcccgcc ctatatgccg ctactaaact aatacatgca atgctgaata agagaacttg
    10621 aagttctaga gaaactggcg gcccgcggac gtagagtaga gctgcgactg cggccagaat
    10681 gagtgcgatg cctccagcga gagcagctat tactgcgagc ggatactgta cgataaaaaa
    10741 tggaactgtt cccgcaaacg ccaatggcag atctgttaca attagtttga gcccgctatg
    10801 attgtatttg aatctggacg ctccgaagat tgctgctgcc aggatagatg ataacagaat
    10861 cgctcgttcg aaatcggtaa cgacaaacag cagcaccgcg gcgctaaaac acagcgcggc
    10921 aatcgccaat ttaaggtgct caacgctacg ttctgctaac caaatagcgg cgatggcagc
    10981 cacaattatc agggtcaatg acatataaat aagatgcccg agcagtggat tgaagtccac
    11041 aattgcaagc agttttcccg gctattcctg ttcggacgcg aaaaagaagt cggacatcag
    11101 ctggaatgaa gcgaccttcc gcgagtacat caggggtccg aaggcaaaag taccgggcac
    11161 gaaatgatct atggcggtct aaaggacgag cagaaggcca acgacctcgt cgcatttctg
    11221 aagcagttcg atgccgacgg gaaaaaaccc ttttaggggc cggccgtgcc gggccctatt
    11281 gcctatggca cttactcgca tagacggcga tctgacccgg cgatggttgc atctacgtcg
    11341 ttccgatttc atgcttggcc ggataggtta gcactccgtc aggatgcgag atcgctgccg
    11401 gcgtaaggct cgccggattg acggtaccga gcctgagtat atgtctcgcc ggctacgaga
    11461 aggtaatccg caatgatccg gcgatggcag cgccaccata atgcctcagc gcacatcaca
    11521 gcgcaacgct gggcgcggct caattccagc agtttggcga agccggaatg aaaactgctg
    11581 tccatcgcgt agtcggcata gttgtgaaaa ctctgatttt cccaatagcc gtttatgcgc
    11641 ggtgtaatat cccgtgcctt cgtacgtcga cctgccagct ctccaatata ctcgtaggcg
    11701 atttgaaagc ccgacaacgt ctcgcgattg tattgcggca tgaattgggt agggttcatg
    11761 tctggttgtt agccgcaggc cggagacagg attatatatg atcttgacag ttttgaatga
    11821 ttcgtaacga tggacgtcaa caaggaacaa gctacgtcgg ggggaatact ttgagcggta
    11881 acacggaagc gatcacgccg cgggctggcg gctctcgcgc cgccccgttg gcggttgcgc
    11941 cgcgtgattc cagattcttt gcgacacaca gaaatccatc actgtcttaa gagggagcag
    12001 cgcggctaac acgtcaaatt tcgaaccggg agggacatac tatgccaatc acaacaaggc
    12061 gtcgcctgct cgttggatcc gctgccgcgc ttgcactgcc ggcgtttgct cgagcccaaa
    12121 gcgaggcaaa gccgcgaata accgccatct cgcagtggtc tgccggcagc gacggcgctg
    12181 cgatctctgc ccttggcaaa ttgttcgagg agcgtggcgg agtctggcag cataatcccg
    12241 tacccggctt caccacagag atgatgaaca agctacgggc gcaaatcatg gccggcgatc
    12301 cgcccgcgat ctcgcagctc aaggggcccg agattgccgc atggtctagg atcgcaccga
    12361 ccgtcgatat cagcccgtgg gtaaagacgg ccggctttgc cgatcgcgtg ccggcggagc
    12421 ttgccaagag ttgccagccg gccggccatt ggatcgcgct gccattgcag gtctatcgga
    12481 tcaacacgct gtttctttcc accaagggca tgcagcgcgc cggcgtaacc aaggcaccga
    12541 cctcctgggc ggaattcaat gatacggcgg aaaaaatgaa ggccaccggc atgattccgg
    12601 tggccaatgg cggcatccgc tgggacgacg gcatgaaatt cgagattgcg ctcgccggca
    12661 tcagtcctga ggtctatcgc cgcgcgatca tgcagctcga taccgatgca ttgaaaggca
    12721 aggaagttct ggcttccttc gaacaacttc gcaaactcgc caactggatg aacccgaaca
    12781 tcgcggcgca aggatgggcc gtgaatctgc cggctttcgt gaagggcgac atgggcatgg
    12841 tgctgatggg tggctgggcg caaggcaatt tgatgcgggc cggcgccacc gatgcggatt
    12901 tcagcagtgg ccccgctccg caggagagcg gtccgccatg cttcgacctc aacgccgact
    12961 gtttcatctt ctggaagccg aagggggccg atcttgacgc cgggcagaaa ctgtttgccg
    13021 agatcgtcat ggagccgtcg acgcaggaga tgtactcgaa gatcacgggc tccattccgg
    13081 cgcgcaccga tgtcaatctg gcggccccgg gctttaccga cgggcagcgt aactcggcgc
    13141 agaatttgcg tgacgccatt gctaaggacc gtgtgctcct gagtctcggg cacaatatgg
    13201 cccagtcgaa cggcatgacc gcggccatga tcgacgtgct gaccgaatac gtgcacaaca
    13261 gcgcaattcc cgccgtcgaa ggacagaagc ggctggtcgc ggcggtagcg agcgcgcagt
    13321 aggaccgtgt cgatgcgaac gggactaact ctacccgcag ccaagcagga tgcggggagg
    13381 gagcagggtg tctggcgccg ccgcttcggc ggcgtcctga ccgagcttgc gatctggctg
    13441 ccgttatcga tcgcggccgg acacgtcgtg gtgttcacgg cgtggacgac ctgggtgtct
    13501 ttcacggcct cgtccttgat gccggactac acatgggtcg gactgcgcaa ttattgggcg
    13561 gtgacccgca cggcgaactt tcagattgcc tatgtgaacc tggtcatctt cggcttcggc
    13621 ttcgtgctgc tgacgatggc gctgggcttg ttgctggcca tcctgctcga tcaacgcgtg
    13681 cgcggcgaaa acgtgctgcg cacgatcttt ctctatcctt tggcggtctc cttcgtggtg
    13741 acgggcacgg tgtggagctg gctgctcaac cccgggcttg gcctgcaaaa gctggtgcag
    13801 aatcttggct ggctggattt ccggtttgac tggctggtgc agtcggacaa ggccatttac
    13861 acgttgatcc tcgccggcgt ttggcagggc gccggctttg ccatggcgct gttcctcgcg
    13921 gggttgcgat cggtggacgc ggatctggtc aaggccgcgc agatcgatgg cgcgggaccg
    13981 tggcggatgt atcgccgcgt gttgctgccg accatctggc cgatttttat caccgtgttc
    14041 gtgatcctgt tgcgctccgc gatcacgtcg ttcgacctgg ttcgggcgct gaccggcggt
    14101 ggacccggta tttccaccat gctgccgacc ctcgtcgtct atgacttcat gttccagcgc
    14161 agcgaactgg gacgcggctc ggcggccgcg gtgctgatgc tgctctcgct ggtcgtggtg
    14221 atggcgcctt acggactcta tgtgcgttgg cagcggcaac ggcaggcaca ggcatgaagt
    14281 ggacacgcgt gatcgtctac gccgcgctga tcatcctggc gctgctgttc ctggtgccgt
    14341 taggtgtggt ggtcgtgaac tcgttgcgct ccagccggga gatcgccgcc acctcgctga
    14401 tcggctggcc gcgccatctg atcttttcca actacggcca ggcctggaac aagttctgca
    14461 tggccgagca ttgctggggt atcgaaccct atgtgatcaa ttccctgacg ctggcgatcc
    14521 cagccaccgt cctctccacc ttgctgggcg ctcttgccgg ctattcgatc tcgctctggc
    14581 gttttcgcgg cgatcagttc gtattcgggc tggtgacgct tggcatcttc ctgccggaac
    14641 aaatgaagtt gatcccctgg gtggtcgtcc tgcgcgatct ctcgctgatg aacaccatcg
    14701 ccggtctggt cctgatccat accgtccagg gcatgagttt cacgacgctg ttttgccgta
    14761 actactatct cggtattccc caggacctgc tgaaagcggc gcgaatcgac ggggccggct
    14821 tcttccggat tttctggcgg atcatattgc cgctctcgcc cccgatcctg atcgtgaccg
    14881 tgatcttcca gtttaccggg atctggaacg agttcctcta tggcgtcacc tttacctctg
    14941 gtgggcagca gccggtcacc gccgcgttga tcgcgctcag cgccgcgatc accagcgagc
    15001 cgatgtatgg cgagcagagc gcggcggtgt tgatcgccgc gttgccgacg ctgctggtgt
    15061 atctgtttgg cggacggtat ttcctgagag gcctcaccgc aggggccgtg aaatgatggg
    15121 ggcatcatgg cagcgttgag cattcgcggc ctgtcgaagc ggtacgccaa tctggaagtg
    15181 ttgaagggga tcgatctcga catcgaaagc ggtgagttca ccgtactggt tggaccatcc
    15241 gggtgcggca aatccacgct gctcaacatc gttgccgggc tggatcgccc tagcgcgggg
    15301 atcgtggaga tcggtggacg ggtcgtcaac gacatcccgc cgaaggaccg cgacatcgcc
    15361 atggtattcc agtcctatgc gctctatccg tcgatgacgg tgcgccagaa catcacgttc
    15421 ggaatggaat gtcgccatgt ccctagggcg cagcaggaga cagccgtggc gaatgtggcc
    15481 cggctgctgc agatcgaacc gttgctcgac cgccggccgt cgcagctttc cggcggccag
    15541 cgtcagcgcg tggcgatggg ccgcgccctg gtgcgtgatc ccttgctgtt cctgttcgac
    15601 gaaccgctct ccaatctcga cgcaaaactg cgcgtcgaga tgcggatgga gatcaagcgg
    15661 ctgcatcagc gcatcggcgc gaccatcgtc tatgtcaccc acgaccagat cgaggcgatg
    15721 acgatggcga cgcggatcgc tgtcatgcat cagggagcgg tgcagcaatt cgccgatccg
    15781 gatacggtgt accgctatcc ggccaatctg ttcgttgcac gtttcatggg ctcgccgccc
    15841 atgaacacga tgccggcgcg gctggtggcg gaagagggcg ggccggtggt cgtgatcggc
    15901 gcggggcggc cggacgaggt ccgccttcgc ctgcagggat acgacgcggc cgcggctttt
    15961 cgcggcagcg aagtggtgct tgggatcagg ccggagtgca ttgccgaggg gagccgcggg
    16021 ttttcgggcg catcgggcgc gcccatcgtc gtcaatgcgc cggttgagat gatcgagccc
    16081 accggcgcgg agaccatcgt cttgctgcgg ctcggtggtg agcctgtgct ggcgcgcatc
    16141 acgccggata tccggcctgc gccgggtggg ctcgccgcct tcgcgctcga cacgcgtcgc
    16201 atttgcctgt tcgacccggt gacggagggg ctgatcgcat gacccaaacc agctatgccg
    16261 gcctcccggg tcgtgtcgtg ttgatcaccg gaggtgccag cggcatcggc gctgcgttcg
    16321 tgcgtgcatt cgcggcccag caggcccgtg tcgcctttct cgacatcgat gccgatgccg
    16381 gtgaagcgtt ggtgcgggag gtggcaagcg agtgcgacgc aactccactt ttcgtgcctt
    16441 gcgacttgct ggacatcgcc gcattgcgcg ccgcgatggc gcgggtccgc gccgcacttg
    16501 gcgacgccgc ggtgctggtc aacaacgccg ccaacgacca gcgtcaggtg ctttccgagg
    16561 tgacgccggc tgaattcgac tggatgatcg gcgtcaacct gcggcacgtc ttcttcgccg
    16621 cacaggcggt agtgccgcag atgcaggcgc gcggcggcgg ttcgatcatc aacatgtcat
    16681 ccatcgcatg gatgcgcggc gtaccggcgt tgccggccta cgccgcggcg aaggcggcga
    16741 ttgtcggctt caccaactcc ctggctcggc tggtcggacc cgaccgcatt cgcgtcaacg
    16801 ccatcgcgcc cggcatggtg atcaccgagc gccagcgtcg gctgtggttc ccggacgagc
    16861 aaaagatcgc tgaagttcgc gcgcgtcagg ctattcccga tgcggtgacg cccgacgaca
    16921 tcgcacgtct ggcgctgttt ttggcgtccg acgagagcca acgtattacg cgccagtgct
    16981 tccaggtcga cggaggcctc ggctaggagg gggattgctg ctttcgatca aaccgtctcc
    17041 tcacccgtcg gcggctgcgt cgcagaatct cgcgctatat cgcagattcg gcgggacgta
    17101 ggcctactgc gtttcgaacc cgcgtcctta gccatagtaa gccttgctat gcggcgagct
    17161 ggggaatcgc ctgttcgacc aatcgcagga atcgggtgtt gcgtgtcccc gtaaccagac
    17221 aaaccgaaac tcccgtagcg aaagctgcgg gagtttcgcg tttccgggtc tacccccgcg
    17281 gctatcccaa cgatttgcgc ttcgatccag ccgaggatcg cgccgaattc gccgtgcaag
    17341 gtggcgtcga tttcgcccgc ggtccgggcc gcaacgcaga agcgtaatcg tctccgccag
    17401 ggttcggatc gtttccacgt agtgctgcca attccagacc ggtcggccaa cattgccaca
    17461 atttccgtca cggctgatgc cggacgcaaa ctccttgaaa ctaggttacc caaccattgc
    17521 ctgcccattg cctgcgcttc gtcactctcg tgggccaccg cggaacggct cgccacgcgc
    17581 ctccagcctt ttcacatctg agtttccgcc cggtgcgagc caagcgatgt cgatggcatg
    17641 atggccgcaa tattgcgatc gtaacccgaa ttctcgcgat ggcccttctt ttgtcttgag
    17701 cccgccattc cggtcacgca tgagacggat tggggctccg gcagagacct cgtggcatcg
    17761 tcagttcagc tgccagcgag ccgatggtga tccaagaaac accggaggaa gcggccccag
    17821 ccgttttttt gtaattcgcc cgacttcccc tccgacctca ggtcgtcgca aaatgttcaa
    17881 aaatctgtca atccgattgc gaatcctggc gcttgccggg ctcctcattc tcctgtcgat
    17941 cgtgatcgcg ggggcgggat ggtacgccat atcacaggtg tcggcggcgc tggcggaaag
    18001 catccgcgtc gccaggcagg cgcaattcgt caccgtcgga atccgcgagt tcgccggagc
    18061 cgatcggcgc gtcgtcagct accttcagac tgcaacggat gacgacgagc aaaggtacac
    18121 gacgcgaaag accgattccg atgcagcgtt cggccaggcg ctcgagctgg tgcgcgaccc
    18181 cgctcgccgg caagccatgc tggacggtcg ccagagcctg aaggattatt ttgccaccgc
    18241 cgacgctatc attcgagatc gcaaggcgct gcgtcaacgg gtgaccagca ttcaggaggc
    18301 gcttgccgcc ctgcccggga gcgcgaaaaa ggagcgggac tcggcacagt cgccggttca
    18361 gcgacttgcc agcatggaca gccttcgccg agcggctcag atcggcccga agagctcacc
    18421 gatccgacca tcatccgcca cggctgccgg tcgaagccca cgggagagcg atgtcgggct
    18481 tgtggccgcg gcttccggga ccgggctggg cctcgcggat gccggtccag tttcggacac
    18541 ggccaagccg gacgtagcgg gcggcgcggc cgggcgccac gccgggctcc tccagcacat
    18601 cggcgaggcg gcgcaactcg tcgcgctcga tccgtccgac gccacgcttg caaactatgc
    18661 cgcggcgatc caggcgttcg gcgatacgcc gccgccgaac gacgccgcgg cccgcttgct
    18721 gctcgttgcc ggggacgcga ccgccgcggc gcgatcgatc ctgcagaact acgacaagct
    18781 tcaggaaaca gaacccgcgt tcatggacaa gctgaaggcg ctgcggctcg aactcaccgc
    18841 attcgcggga gagattgcga atggcgcgat cgccaccgcg gcgtcgggga ccagcgaagt
    18901 catctacgcc agcgcatttg ccgtcgtcgc cggcagcctt ctcgcatggt tcatcgtgcg
    18961 cggaatcatc gtgcctctga acgcgatgac gggtgccatg acgggcctcg cggccggaga
    19021 cggcacggtc gatatcccgg aaatgcgcag caaaagcgag ctcggcgcca tggcccgggc
    19081 ggtcgatgtg ttcaaacaga atgccgacaa gatcgccgcg atgctcaccg ccgaaaccac
    19141 cacgcgcgag atcggcgagg tcatttccgg ggccgccgcc ggcgatctca cgatccgcgt
    19201 gccgctggac aacaaggttg ggttcctcaa ggacatcggc gaacaggtca atcgcctgct
    19261 caatgcgacg aacactgcgt tcaaggaatt cggcgagaag gcgcgccaca cggcgatctc
    19321 ggtcggtgag gcgagcgcgg cggtggggca ggtctcggat ggtgcacgct cgcagagcgc
    19381 cgcgttgtcg caggtcgcaa ccgcgcttcg ggagtcgacc gacgcgctca agagcgtctc
    19441 cgacaacacc aaggctgcga gcgacaaggc gagcaccgcg tcccagctgg tccagaaggg
    19501 cctcacatcg gtcgagcggc tcgctgacat cgtcgaagcg atcgcgcaga atagccgcaa
    19561 ggtgaaccag atcacgcagg tcatcgcgca gatcgccaat cgcacccaca tcctgtcgct
    19621 caacgccgcg atcgaagccg cgcgcgcggg cgagcacggc aagggcttcg tcgttgtggc
    19681 gcaggaggtc ggcaagctcg ccgaaagcgc cggccagaac gccaagcaga tcaccgacat
    19741 cgtggagcag gcgacgatcg actccaatga gggcaagtcc gctaccgacg tggtccgcgg
    19801 cgccatgcag ggcatcgccg cgcagaccga tcagacaacg cagatgatcc attcctcggc
    19861 cgcggccgtc gaagaacagc aggcgaccat tacccagatc gacagcagcg tgtcacagct
    19921 gcggtcgatc gcgacgtcga attcctccgc cgccgaggaa atcaccgcca ccatggtgca
    19981 gctctcgcag cttgccgatg agacacgcgg ccggctcgcc caattcaaga ccgcatgagc
    20041 agttgcgttg aacctaggga gcaaaggcgg catgaacctc gccgaaatcg aaccggactc
    20101 catggccttg cgcctcgcgg aggtggtcaa ccggcgggtc ggcctgaggc ccgccgatgc
    20161 ggtccgaaag gtggccctaa gcctcgcctg cgtcgaggac gcgcaccgtc tggcctatgt
    20221 cgagcggatg atcgctgcgt ctccgcgcga tcgcgactgg atcgatttcg tcgagcgaat
    20281 gctggtccac gaaacctatt tcttccgcca taccgcccag cttgatttgc tgcgtgacga
    20341 gatcctgccc cgcctcgacg cgcagcgccg cgaggcgggg cgacccatgc tcgtggtgtg
    20401 gatcgccgga tgttcgaccg gcgaggaagc ctggacgatg gcgtttctgg cgcacgcgcg
    20461 cggcgccggg gtattcggcg aggccgttcc ggtctcgatc ctcgccaccg atctcagcga
    20521 gacatcgctc gccaccgcaa ggcacggcgt ctacgaccgt ctgcacgggc tggacagctt
    20581 tcgcgccatt ccgctctggg ccgcggctta tttcgggccg ggccgtggga cgaacgcctg
    20641 gatcgttccg gagatcctgc gcggcagcgt acgcttccag cgtcacaatg ttctcgatcc
    20701 ccctccggtc gccagcgccg atctcgtcct gtgccgaaac atgctgatct atttcgacga
    20761 ggccgccaac cggcgtgccc aggaaaacct cgtgtcggcg ctgcggcccg gcggcgtgct
    20821 cgtgctgggt ccggccgacg cgccgcggac aatgggtggg ctggaagcga tcgggacgca
    20881 ttcggcaacc gtctatctca agaccgcgga ggtcgcgtga gccgcgtcct ggtcgcttcg
    20941 ccgtcggcgt tcctgcgcat cgggctgctg cagcaattgc gccggcgcca agggaaccaa
    21001 ggcaacatcg tgcccgccga agccgcaacg gcagctgacg cggtgcgaat tctcgcaggc
    21061 ggcgggatcg atctggccat cgttgatgac gccctgatgg tgaccccggc cggcgatcgc
    21121 ctggtcgctg cgatcgaacg gcaccatgga cgcaccctcg tgatcgcgac gcgcgagccg
    21181 caagggacgc agcctgcgag cagcgggacg ctgcgggtga tctccggcgt tgtcgatggc
    21241 acgctcgaca tcggcgcgat cgcccgctcg ttacccatgg cgctcgacga ggtcgccgtc
    21301 gcgcggggca ttcgacgggc gccggccggc gctccgctgg tgccgatcgc cgagccttgc
    21361 gcccgcgcgc cacgcgggcg gcccgacatg atcctggtgg cggcatcgac aggtggtccg
    21421 agcgcgctca cgctgctgct gcgccatctc ggtccgccgc ggttgcccgt ggtgatcgcc
    21481 cagcatatgc ccgccgatca gaccgccggt ttcgcgcaat accttgccgc cgaatccggg
    21541 gtcgacgtga tcgaacaggg cgccggagac gtcccgtcgc aggcgcacgt gatcgtgctg
    21601 cgtggcggat cagattatcg tctcgcgcgc gggcgcgccg ggcaattccg cctggtcgcg
    21661 accgaagtcg cgggcaatgt cttccacccc agcgccgacc tgttgttcgg ctcggccgct
    21721 gaagccggca tgacggtcat cggcgtggtt ctgagcggca tgggcgacga tggcgcccgg
    21781 ggcgccgccg ccatcgcggc ccaaggcggc cgggtgctcg tgcaggattt tgcgagttgt
    21841 gtcgtcgccg ggatgccgtc ggccacgcgc gccgtctgcc cgtcggcggc ggttgcgagc
    21901 gtgcccgaga ttgcagagcg tttgtctcga tggtcgatgg cgcgcatcca ccgggccgag
    21961 gttgttccgg agggctgaat ggcctcgctt ctgctggtca ccgaggataa cggtttcgcc
    22021 actgccgcgg aggcgctgtc gaatgcggat ctgaccgtca cgcgtgcgca tgcggaggag
    22081 gcggtcgcgg tcgcgaaaca tgaccttccg gacgtgctcg cgatcgacac ggactcgatc
    22141 gtcgaggcga agccgctgat cgcaacgctc tcgctcgtca cgcggtcgat cgtggttgcc
    22201 gtggcgcgcc aggcatggcc gggctcggaa gccgccgacc tctggcgaga tgcgggcgcc
    22261 gacgtcgtat tgccaaaacc ttccggggcg gcctcgccga gcctcgcagg cgccgaccgc
    22321 gaggcctatg cgcaatggtt cgcggatctg gttcgcaccg gggagaacgc cccgtgagca
    22381 atggtgccga ttcgcagctg atggcgcaat tcaacgtcga ggtcgaccag caccttgcgg
    22441 cgatcgagcc cgtcctcggc atggccgagc ccgacgcatt gacgcgttcg gacatcgatc
    22501 tgctgttccg ggaattccat tcgatcaagg ggctcgcccg cgtggtcgca gcgaccggca
    22561 tggagcgttt gacccacgaa gccgaaagcc tgctgtcgcc ggtgcgatcg ggcgatcgcg
    22621 cgttcgacac gaccattcag gaaccgttga tcgcggcgac cgatgccctg agggatgcgc
    22681 tcgccgagcc gctcggatgg cgggcgccaa tcaagatcat cgaggccctg cgtgcggcga
    22741 cggctgcgat cggtcccgac ggttcaagtg aaaacacctc cgacgatgca ggcaacgaac
    22801 cgtggcgctt tctcggcgac gatcccgacc tgctgcgggc tttcgccgaa ctgctggacg
    22861 aagtccttcc agcagccgcg caagcgatcg tcaatcggga cgaagacggt ctccgtgagg
    22921 caatcgacgc cgtcaccttt gcgtgcacgc gcctgggctt cgacggtgtc gggttcgtcg
    22981 cgcaatgcgt cgccgttcaa accggcgccg aacgcgtgtg tgccttcacc aggctgatgc
    23041 ggaaggcggc acgcttcggc gcgctggtgg gcgaggactg cggtgtaacg ggcgcggccg
    23101 tcgtgcttca ggatctgctg cgagccacgc tcacgacatg tctcgacgag gcgctggcgg
    23161 tgcaacccca ggcgcggcgg gcgctgctgc gacgttgcgt cccgctgtgc gatgtcgcgg
    23221 ccccgaattc gggagccggc cgcctgatcg aggatatcgt ggaggatctg ccgggctcgg
    23281 tcggccatcc gcgggaccgc gaggcgctgc ttctcgaggg gatcgccgcc ctccggcgcg
    23341 gcctcggcag tgaacgggat gccgatctgc cggtcgcgac attccgtgac gcgatctgcg
    23401 ccccgtaccg cgccagcgag gagatcgagg cgcatttgag aaggctcggc ctcgacacca
    23461 ggctgttctc gttggggccg ccacgccggc tcgaacggtt gtccacgctg ctcgacgggt
    23521 cgggcgcgac cttgaaggaa gtctttgtcg aggccgtgac gccggcaagc ctcgcaagcc
    23581 tcgcgcggct cgatcccctg ctcggccaga gcgccatgcg cggcggacgg cctgggatcg
    23641 ccgtgctgct gctggatgcc gacccgccgg gcgcagtcgc cgaaaccgtg gcggatggcg
    23701 tcggcgaatt gcgccggctc gatccgggac atgtgcccgg cgaattctcg gtatcgtccg
    23761 ggtcggccac gggaggcccc gagcgcgccg gggagacgca agtgcgggtt ccggtcgcgg
    23821 tgctcgacaa actgttcggc cgcgtcggcg aattcttctc gatcggcagc cggctcaacg
    23881 tgctcgccac cgaaaccgac gtgccggacg cgcttcggcg tctgtcggat ttcgccgtca
    23941 cgcgcgcacc gcagctgcaa gccgacatcg aaaccctggc gcggcagcat cgcgacttca
    24001 gtacggtcga ggccgacgtc ggacgcatcc tcagcctcat tcacgaaagc acgctcggcc
    24061 tgcgcgtcat tccattggaa atgctgacag ggcggttccc ccgcatggtg cgcgacgcgg
    24121 cgcgtgcgca tggcaaacag gtgcgctttc tggcggacac gggtggcatc cgcatcgaca
    24181 agggaatgtc ggacatgctc gccgatccgc tgacgcacat gttgaccaat gcggtcgagc
    24241 acggcgtcga gcccgaggcc gagcgcctcg cccagggcaa gccgcgagcg gccaacctgc
    24301 ggctgatcgc ccagcagcag ggcaatcgca tcgtggtgga gatcagcgac gacggtcgcg
    24361 gcatcgacat cgaacgcgtc aagcggcggg cgatgtcgtc gcgcctcgct tccgaagacg
    24421 agatccgccg catgagcgac gagcagctcg cgcgcttcat ttttacaccg ggcttttcga
    24481 ccagggagga gatcaccgac gcctccggcc gcggggtcgg catggacgtc gtgctcgtcc
    24541 acgtcaccaa gctcggcggc aagatcgaca tcaccacgca gcccggtcgc ggaacgacgt
    24601 tcaggctgga cctgccgctc tctgcggcga tccagccgat gcttctcgcc gacaccggcg
    24661 ttcagtcgat cggttttccc gaagcgatgg tcagcgaggc gctgatgttc ccgtcggccg
    24721 gcgtgcaatt cgtcaatggg cagcgcgcga ttctgctgcg cgagcgcttc ctgccggtgt
    24781 tcagactgac cgagctgctg cgtctgcctc cttccaccgc ggcgaatgct gcgtatctgc
    24841 cgatcgtgct gtgccaatgg gccggtcagc gcatgggcgt cgaggtccac cgcatcctgc
    24901 gccgcggcga aatgctgatc cgcgaggcgc acccgcgcgt cgcagcactt cctggaatcg
    24961 gcggcatcac cagcatgggt ttcgaccgca tcgtcctggt gctcgatccc gaggggatgt
    25021 tcgagctcgc ccgcgaggcg tcggtgtctg gattgcaggt tccggccgaa cggctggcac
    25081 tcgaacaggg gagtgcggcg tgagcgagtt tctcgaagtt cgctgcggca tgcaccggtt
    25141 tctggttccg agtgccgaca ttaattcgat cgaggtcctc aagggcgttc cggagccgtt
    25201 cggcggcaga cgcgcgccgc gcgagtggct gctgctggac ggccgcgttc tcgctgggga
    25261 cgatgccggc gaggctaccg agcatggggt cgcgctgcgc gcgactgaag gcacccctct
    25321 cgagacccgt atcgtcgtcg atcaggttgg cgcgctggtt cattgcgatc cctcggccat
    25381 cgaacccatg ccccacgcgg tggcgcgcct gcgggacctc ttctccggca tttggcgcga
    25441 ggaggaatcg caggaatatc tgttttgcgt gcggccgcgc gacgagcttc cggtgcagcg
    25501 cttcgtttgg cgtcgccgga tccgacgcgc ggtgatgaca atccgtccgg caaccggcga
    25561 cagaaaggct tcgacatgac cctgcgggca gctccggccg cgtacactgc agcgcgcaag
    25621 cgcgttctcg tcgtcgatga cagcttcatc atgcgcaacc tggtgaagga gatcgtcgaa
    25681 agcgatcctg atcttgaggt ggtcgatacg gctgaaaacg gcatggtcgc cctgcagaag
    25741 gtgcgccaga tcaagcccga cgtcgtcctg ctcgacatcg agatgccgga aatgtccgga
    25801 ttggagacgc tgcggcgcct cgggctgcgc tccccctgca aggtcgtcat cctgtcctcg
    25861 ctggtcggca acacggcttc ggccgagcgg atcgaagccc tgcgtctggg cgcggtcgcc
    25921 acgatcgcaa agccgtccgg aggcgtgagc ctcgacctca agcaaaagcg ggcgtcgcag
    25981 atcgtcggtg tattgcgcaa aactctgggg ttgccgacca tcgtcgatgc gacgccggcc
    26041 gaaatcgaag atcaggctgg tccctcgatg aggttgacgg atctttcgcc cgggccgatc
    26101 ggcgaccaat tgctggaggg gctcgagacc ggcgtgctgg tgtttggccc cgatggatcc
    26161 ctcgtccggg ccaatccggc tgcgggccgc atcctctatg gctgcgatgt gaacccgaac
    26221 cgcaccacga tcgggggctt ctgcgatgaa ttcaaccacg cgcttggcca ggaaatctgg
    26281 gacgtgatcg ccggaggcga agccttagct ccggcggacg tcaatatcgc gacaccggac
    26341 ggcgactgga tagcggtacg gcgcgcgatc cggccgatct tcgtttccgg caagccgtgt
    26401 ggcgcattgg tgctgctcga cgacattgcc gaaaaacgcc ggatgcagtc gctgctggaa
    26461 aaaaccatgt cgtccgacgt cgccagggcc atgatcgata ccccggcggc ggaactcggc
    26521 ggcgccatgc gcgacgcgac gatcctgttc gccgacatcc gcggcttcac gccgctcgcc
    26581 gaggccctcg gcgcacgggg ggtcctcgac ctcctgaacg aatatttttc ctacatggcc
    26641 gacgtgatcg gcgccgaggg cggcatcatc gacaagttca ttggcgacgc cgtcatggcg
    26701 ctgttcggcg ttccgacgtc tcatggcgac gacgccgatc gcgccgtggc ggccgcgcgc
    26761 aacatgcatc gcgcgttgac gctgatcaac gagcgtcgtg cgggacctgc gctcaggatc
    26821 ggcgtcgggc tcggctcggg gtcagtcatc gcgggccagg tcggttcacc caaccgcatg
    26881 aactacaccg tcatcggcga cccggcgaac ctcgcctccc gcatcgaggg cgtcaccaag
    26941 gcctacggct gcggcatcct gatctgcgac gagacgttca aacggctcac ttgtccggtt
    27001 cctgcccgca aggtggatgt catcatattg aacggccggg agaccccgac tgcgatctac
    27061 gaagtattcg tcgaagaccc cggcgccgcg gcgaccgaat ggctgggaga attcggtgcc
    27121 ggcgtcagcg cctatgttgc cggcgatttc gttgccgcgc aggcgcacct ggcgcgcgcc
    27181 atggccatca atcccgacga tacgacggca ggcgtgctcg ccgggcgctg ccggcggctc
    27241 ggcctgcggc ccgcaggcga atggaccggc gcatggaaac tgaccgagaa ataggacggc
    27301 gcgatggagc ggctcgattt cctgtgcatc ctggccggcg cgcgccgggt cgcgatcccg
    27361 ctcaacctcg tgctgtcggt ggaggaggcg cgtccattga caccgctgcc cttcagtccc
    27421 gaactggtcg aaggcctcgt catggcgctc ggcagggtgg ttccgcagat gtcgctggcc
    27481 gccgtgctgg gcgaggagaa gctcgaaggc ggagtgctcg tggtggtcgc ggcaagcgac
    27541 gatatccggg cgctgcgtgt cgatcaggtc gccggcatgg tccagatcga catccaggcc
    27601 gtgaagacga ccgacgaggc cgagcgctcc gcgcaatcgc tgctcaccgc ccggttcgag
    27661 gcgctcggcg ccgaatggca cgtgctcgac tattccgggc tgactttcga cgagcaaatg
    27721 cacccagccg aaggcgctga tggcgcggcc ctcgtagcgg ccgcgggcga ggatgccgac
    27781 gacaccccgc cgcccgtcga ggatgtcgat cagcacctgc cgcttctggt cgtcgagatc
    27841 gccggcgaga gctatgccct gccgacgtcg gacatcgtcg agcttctgat cccgggcgtc
    27901 gtgcgctcga tgccggcagc gccggcctgg gtcgccggga tgatcgaccg gcgaggtgct
    27961 ccgcttctgg tgctctccac cgccatgctg ctcggacgtc cttcgacctt caaatccacc
    28021 atcacgctgg tggtgaacat cgcgggcgcg ggcgaagtcg gcatgatgat cgaccgtgcc
    28081 gtcggcatcg agcgcgtgca ccgttcagcc atccacatcg tgccgcgaga catggcaggc
    28141 gtggccaact atttcgtcat cgagaccgag cacatcgtcg gcattatcga tctggtcgcg
    28201 ttgaccggac aggtcgggga cgccgtcgca gagctcgcgc cacgcgaacc ggatttgccg
    28261 acacctgcgc aaatcgtcgt ggaatccgca aaggcttccc acaagcttct agcggttcgg
    28321 ctgggccgcg aattgttcgc gctcccgctc gacagggtcg aacgcattca ggcctccatc
    28381 gtcctgacgc cgttgccgca accgggaacg ggctttcacg gtatggcgga tgtcggtgac
    28441 acgatggtcc ccgtgctcga tctgcgtcgg gcgatcgagc atgccggctc ggatccgaat
    28501 ccgccgtgca cgctggtcca gatcgaaggc gcgctcgccg ggctcgtggt cgatcaggtg
    28561 ctgcgcatcg aggatattcc ggacaacgac gtcgaggata tcacgatgaa tccccgcctc
    28621 ccggtatcgc acgtcgcccg ctccggcgac caattgctgt ccgtattggt cctcgaccgg
    28681 gtgttgccgc cgctcggcag tatcttggta gcggccgcga aaccatgaca ccgcgtcaat
    28741 ccaccgggag gccgccagca ccccctaagg ttcgacagcg caaggcggcg ttctgaaaac
    28801 tcaacgtcga cctcgatcag cccggacact gcgccgccgc gcccaacctc acccccgcgc
    28861 gacgcgctgg cgggagcccg ggcggtaggc gagcctggtg tggcccgggc aatagggcag
    28921 gccttcgacc ggcgcgttgc cgcagaaaca gaaatcctcg gcgcccgggg tgctgatcgg
    28981 ccagcggcac ttctccgcgc tcaactcgag caacgagcag gtgtgcccgt tgtggatcgg
    29041 ctcgtcgtca tcagcgggca gcggctcggc gtacagcgcg cgcagcatct gatattgcag
    29101 cctcggcacc gctctgccgc gcggtgcctt cggggtatcc tttcgcgccg cgcgtcgcgc
    29161 ctcgccgttt ttttcgcggg tcagattgag gcgggagagt ttgccgatga cggcgttgcg
    29221 gctgaccccg atgtcgcagg caatttcgcg gcaggacagc ccggcttcaa aacggttttt
    29281 caagagttcg atgcgttcaa cagtccaggt gggttggtgg gcgagcatgt tggttcccca
    29341 agctacgagt gatcggtccg aattcttcgg cctcgttgtg gtccgaaccg tcgcgttccc
    29401 atgctcccgt cattgcgtct tgccgttgtc gcggatcgca agcagcccgt ccttgatggc
    29461 gagccgaatg ccttcctcga tcagggtctt tgcaacgccg ggaacgctgg tgccgtgagt
    29521 gccctgtctc accagttttt cgagataaga gatggtcgag agcgctaacg tcacgggaac
    29581 acggtcggtt tcggcttttt ctgtggccat gacaaaaaac tagcctttaa aaggtgtact
    29641 gaaaagtaac gatttagagt ctattaggat tcaattgtgg gcggaagccg gaggccgatt
    29701 tttctttgtc atcttggtga atccttgaat tcattgaaaa aaatcgccgt cgggcggtga
    29761 tatggaccgc ctggcggaac ctaaggaaaa gggcatctgc tccgggttgc cttctcggga
    29821 acctccggac ggaatcaaag tgcacccact ccaggtgcaa tgcggcttga ctgcaacgac
    29881 tgagcgtgtc cgcactattt gaaaatgttg gctcgtgggg gagtggtggc taagcacgat
    29941 acgttcagcg cagcggcgtc agccgccggt tactattacc aagcccggct ggcgttggcc
    30001 gaagcgctgc gctttgcata tgcagaatct ggtgtcgagg tcgcgattga aaagctcgac
    30061 gatgtatctt tcgaaagcgc cgggagcccg ctagagctgc tgcaatccaa gcatcacatt
    30121 gccaaaccgg cagaccttac ggactatagc gccgacgtgt ggaaaacgct ccgcgtttgg
    30181 tcttcacggg taaagcagga cccttctctt ccaagcagaa cgcgtttcgc attagttacc
    30241 acttccgctg catcctcagg cagcgcagcc gcattacttc gccctagcac cccgacatcc
    30301 gatcgaaacg ttgacaaagc aacaaaactc ctcgttgcgg tagcaaaaga atctagcaac
    30361 gcggtgctaa aaagttcgtt cgatgaattc ttgtctctca catcggagat gcaaagatca
    30421 ctgctcgacg ccgtcgacgt tcttgatgcc tctccgaata ttgtagattt ggaaagtatc
    30481 attgaagaga gactgaagat gatagctccg cgtggcaaaa caaagctagc gcgggaacat
    30541 ttggagggtt ggtggtggcc ccgaatatgc aaggcattgc tggagccagg ggtaggaaaa
    30601 gtgtcaattt tagagttgga agcgcgtcta gatgatattc gcgaaatcat gaaacgcgac
    30661 gcgctgcccg tcgagatgga caacgtcgaa gtagacgatc agcaccttga agcactcgac
    30721 gaactgacat tcgtaaggca gcttcgactt aatcaagctt ggtcaagcac gcatcgaact
    30781 tgcaaagcgc gatttttacc gtgcatccac gcaacgatcc cgttggaccc gtgagaattt
    30841 gctttttgat ggtgaggtaa gcgcgttcga aagaacgcta gtcgaagaat ggcagcctcg
    30901 ctttcacgcg atgtgcgaca ccttagcaaa aaactgcgcg gccgcagcgc tccgaaaggc
    30961 aggccaaagc ctgtaccaat gggtggaaac cggcgctcgc ttttcgtttc gtacagtttc
    31021 gcatcggttc ttgagcgtgg gttcattcca catgctgtcg aatgacgttc gagttggatg
    31081 gcatcgcgac tttctcaaaa ctttcaaccc atccaaagag aagtgaaatg gctttagcca
    31141 aaagatcatg gggtttggcg tggggcgacc gaccgcgcga ggaagctact aaccttaacc
    31201 cggctttttg cggcgaatta attttccgct cgattgccga attccggcgt ttgcgcgagt
    31261 tgccgttccc attcgcattg tcatttgtgg tacttccgct cgtattgcac aaaaggacgc
    31321 gcgatcagct tcccgggacc gcctctactg cgtttgttgg atgggtcgcg gaacatggcc
    31381 cacttttggc tgaattgccg caacgagcac ttcatcttgc gccgattaca cgcgaggcga
    31441 taatgttcgc aatgcaacac gagttgttgg ccgtagaaca aggtgggcta atacctggct
    31501 caaagccgat cgcgtcccgc aaacgacccg aacgaacgac ggatgatgcg gatgatgcgc
    31561 gacgctcggc gggactcata ggacgctggt ttgccaatca gggtctgtca tcgtccatca
    31621 tgcaaggcct aggtgtaacg ccatgaccct gcagcttcgt tccatttcga tttattctca
    31681 cgatgagcag cgccgcgata tcgtcttcaa attgggcgcg ctaaatattg tgacaggagc
    31741 gtcaaaaaca ggaaagtcag ccttactcga tattgttgat tattgttggg gtcggagtga
    31801 ttgcactgtt ccagaaggcg aaattcgaag gaaggtttct tggtttgcaa ttcatctgga
    31861 caacgccggg gagggcattc ttttggcgcg aaaaaatcca ggacctgcag gtcgaaccag
    31921 cgacgaaatt tatctcgaac gaggcgttga agtgcttcct gtaacgacaa cgtcatttca
    31981 gaaaaacata accggggatg gacttcggtc tcaattatcg gtcgtgttgg ggatctcaga
    32041 aaacttgcac gttccgccgg acacggcttc tcgccagcct atggaagcat catcaaggca
    32101 tgccatttta ttttgccttc aggctcagga cgaaattgca aatcgtaaat ttctttttca
    32161 tcgccaaggg gagcagtttt tcccagcagc aatcaaggac tcattgccat atttcttagg
    32221 cgcggtcgat gaggcgcatt ttctcgcact tatgcgctac aatgatgcgc gtactcgatt
    32281 gaggagagca gagcgtgacc aattggaata tcgctcggca gttgatgcgg cctctaacac
    32341 agctttgtcg ctcgtggatg aagctcgccg cgttggctta atttctcgag taggcggttc
    32401 ggatgcggcc agtactcatg tgcttctaac tgaggcggca atgcctcaac cgatgaatct
    32461 cgatcttatc gatgacccaa cagcggatat ggagcaactg gaggagcggc gtcggaggct
    32521 tcgcggtgat ttgcaagaag ttcgtgacga tattgctgag gtagagcgcg ctaatcgaga
    32581 tgcatcggaa ttcgagactg agacgaaaga gcaggaagcg cggcttttgt ctcttgagct
    32641 cattcgggga catgaatctt tccctgacca ctgtccgctc tgcgacagcc agctgactgt
    32701 tgctgttcca accgtggatg aaatacggca atctctcgca agtatcggca cccaactcac
    32761 ttcagtgcgt cgggactcgc cacgtctcca ggagcgcctt gctttattgg aagcgcgccg
    32821 cgctgagttg gaagagctgc ttcgtgttgt gcaacgtcag atagctcaac gcattgcgga
    32881 caatgagcgt ttgcggattc agcagaacca attttcagag caggcccgcg ttgctggacg
    32941 gattgcttat tatttggaga gctatacttc gatagggcca acaagcgacc tcagccaaac
    33001 aattgatagg ctcaaagccg aaattgctga gctagaaaaa gtcttggatg atgatgcgct
    33061 cgaaacgcgt atcgacaccg cgcttagtct ggtggcacag gaattgacag cgtacgcaaa
    33121 tagcctttca cttgaacatg gcgcaaatcc tttgcgattg gatcgcaagc atttgacggt
    33181 tgtcgctgat actgtcgatg gaccgctgtc gcttgcgcag atgggaagcg gtgagaattg
    33241 ggtcggctat cacgttgcgg cacatcttgg tttgcataaa cttctgcggc gccgaagcag
    33301 accggtgccg gcatttttga tgctcgatca accctctcaa gcacattatc ctccagaaca
    33361 cgacgctaat ggcctaattg ctgggcttgc ggatgaggac caagctgccg tccacaggct
    33421 cttcaaattg ctatcagcat attgtgttga acttactcca aggatgcagg ttatcgtttc
    33481 tgaccatgtt gagttattgg atgattggtt ccgcgatgcg attgttcaac gctggcgaga
    33541 tggaatcaaa cttgtaccag tcgattggtt gactgcgata aatggctgag catggcacgt
    33601 gatccgatta catgggacag ccaaactggg cattacattc cgccgaaact gacgtggcag
    33661 gtcgacgccc ttgaaacagt tggcaacgtt gatgctccag aggtgtggtt gagcaagccg
    33721 ggaggcgagt atcgattggc agcatcattc cattttccag ggtttaactt cccatccggt
    33781 gctggtcttc tccctagcgg cattgcctac tctccatcgt cctttgtcct tcatcatgca
    33841 caagatccct ataacagttg gaacgtgaag gcgtcgacaa agcgctttag cggcccgatg
    33901 gagcattggg attggctgcc cggaatcatg ttggttgacg agcggaccgg ttactcgctt
    33961 ccgtcgaatg agctaagcgc ggtcgcagga actgagtggg cggacaacga tgcgcgaata
    34021 tttcatggtt tgtacgatga tgccaaatat ggggagcacc tcgatgtgtt tttgcaggct
    34081 gtgaacgcga tcacggggcc ggggtgggca aactggcctt tcggccatcg ccagctggag
    34141 catgcagtca tgtgctggtt gcgcccaggg gtcgctctgc ctgatgtggt actgctcctg
    34201 agcgtacgtc agtcgggcgc caaattttcg tatcggcgga aaggtcttta cgatcccggt
    34261 gaaaggctgg gggcgtggtt gcagagtccg cctaaatggg actgtcgcca ggcgatgcga
    34321 tttttgtatc aagccaacct actcgccaag gatggcttac cggagttggc tgttgtgtcc
    34381 gcaattgcgg ctcttgagaa tgcgagcgct gaaattctgc tttaccttac cggtggtaac
    34441 gcggcagtag tccagtcggc attcggcaag tgcaaattcc ttagccgatt tgacaaggtg
    34501 ctgcccgcct acggaccgaa attgccatcg gccgagttct cagcggtaaa ggcagcctat
    34561 ctagctcgca acggggtggc gcatgcgctc gcgccggtaa agcaggagga tgccattcgg
    34621 cacgtcaagg cggtagagga tgtgcttgcg tggtacatcg ctaacggggg atagttatta
    34681 caaaacctta tcgagaagcg gatcgatggt ggctgagcgc gattggcgca aaatcgcaat
    34741 ggtaatcttg ggatattgag aagctacaaa gatcggaatt cgatgccgat agcggtaaag
    34801 caaaacatgt atcccgattc aaaatcgcgt tcccaaaagc tctacgatcg cgctttgacc
    34861 agcctgcccg gcggcaactc ccgcaccacc gtgttcatga agccctatcc gatctatgcc
    34921 gcgcgcggcg agggctgccg ggtctgggac gtcgacggca acgaatatat cgactgcgtc
    34981 aataatttta cgtcgctgat ccatggccac gcgcatccct tgttggtcga agcagctacg
    35041 cgacagctcg cgctgggatc ggcgttcggc ctgcccactc tgtccgagat cgaattggcg
    35101 gaattgctgg tgtcgcgatt gccgtcggtg gagcaggtcc ggttcaccaa ttccggcacc
    35161 gaagcggtca tgatggcgtt gaaagccgct cgcgcgttta ccggccgccc aaagatcgcc
    35221 aagtgcgagg gcgcctatca tggttcctat gactacgccg aagtgagcct tgatccgacg
    35281 cccgaggcgt ggggccgcaa tgccccggtt tcggtcgcct atgccagggg cacgccggac
    35341 aatgtgctgg ccgatgtcgt taccatcccc ttcaacgacg ccgaggccgc cgtcagcctg
    35401 atccgcgagc acggttccga gcttgcctgc gtgctggtcg acccgatgcc gaaccgcgcg
    35461 ggcctcgcgc cggctgacag agcctatctc gaggcgctgc gccaaatcac ccgcgaagtc
    35521 ggggcgctgc tgatcttcga tgaagtcatt acgttccggc tggcctatgg cggcgcgcag
    35581 gggctgtgga atatcgatcc cgacctgacg acgctgggaa agatcatcgg cggcggcttc
    35641 ccggttggcg cgataggagg ccacaaggaa ttcatggcgg tgttcgatcc gcggtccggc
    35701 aagccggcgc tgccgcatgg cggcacgttt tccgccaatc ccgtgacgat gcgcgcgggc
    35761 cttgccgcga tggaattgct cgaccatgcg gcgttcgtgc gtctcgacat gatcggcgcg
    35821 gcggtgcgtg ccggcatcaa cgaagccttc cgccgttatg gtgtgccggg cggcacggtc
    35881 gggctgggtt cgctcctaaa aatccatttt gccgatcgtc cgatccgcga ctaccggtcg
    35941 gcctacatga ccgaacagga agtgctgcgt caggcgatct tcaatcgggg attgctcaat
    36001 cgaggcgtgc tggccgcggg ctacgggttg atggcgctct cgacgccgat gaccgatgcc
    36061 gatatcgata cagtcatcac ggcggcgtcc gatgcgctgg aagaggtgtc cgcctcagcc
    36121 tgacgacccc tccgtcaacc gacgcaaact ttttttgcca gcaggagcgc atgtccccca
    36181 agatcgatcc catcacgcga tccgtcgttc agcatcgtct cagctcgatc gtgaaggaaa
    36241 tgggcgaagc catgcttcgc acctcctatt cgcaaatcct caattccagc cgcgatttct
    36301 cgcttggcat ttgcgacaca cagggccgcc tgatcgcgca ggccgatcac atcccggtgc
    36361 atgtcggcgc gctgccctgg gcgacgctcg cggtcgaagc gcggttcaac gacgtccggc
    36421 ccggcgacgt tattctgctc aacgatccct atcatggcgg cagccatctg cctgatctga
    36481 ccgcgttcgt gccgatcttc gacggcgagc ggcggttgct ctggaccatc gtccgcgcgc
    36541 accagagcga catcggtggc gccacccatg gcggctacaa tcccggcgcc accgagattt
    36601 ttcaggaggg gctccgcgtt cccccgatca agctatacga atctggccgg ctgcgcgacg
    36661 atctgcttga cctgctggcg ctgaacattc gtaacccacg cgagtttcgc ggcgatctgg
    36721 ctgcaatggt cggtgctgcg catctcggcg agcgccggct gtcgcggctg tttgcggaat
    36781 tcggcgcgcc ggtggtcgag gcggcggtcg agaccattct ggatgcggcc gaagcgcaaa
    36841 cacgggcggt ggtctcgacc tggaaagacg gcgtgtttta tggcgaggca ttcctcgatg
    36901 acgacggtca tggccgcagc gacatcagga tcgccgcccg ggtcacgaaa tcgggcagcg
    36961 atatcgaggt tgacctctca gactccgatc cgcagtcgac cagttttgtg aattcctcgc
    37021 atgccaacat gcaggccgcc gtggtgatgg cctttgcgta tctgatcgac gccgatattc
    37081 ccaaaaatgc cggcgcgctg cggccgctca aggtgatcgc gaaacagggc accatcgtct
    37141 gggccgatcc gggccggccg gtgacgctct gcaccagcca tccctcgaac gagatcgtgg
    37201 aagccatcgt caaggcgatg tcggcctcat gtccggaccg cgccatggcg ggatggggcc
    37261 gccgcttccg gatcgcgatc cagggagaag atccgcgttc gggacgaaac tttatctggc
    37321 acttgtttca ggcccgtccc ggcggcggcg cttcctccgg cggcgacggc tggtcgtcga
    37381 tcggcgaatg gcacagcgtc ggcggcatca agttcggcag catcgaagtc gccgaggtgc
    37441 ggtttccgct ccatttccgt caccatgaat ttcgcccagg ctccggcgga gacggacagt
    37501 ttcgcggcgg ttttggtgtc gcgctcgatc tcgtgctgga aacggcaaaa cccgcgctcg
    37561 ccaacaccgc gggcgatggc gcgcgccacg gaccctgcgg aatgcttggc ggcgaggacg
    37621 gcgtgccgca cgattaccgc ctgatctccg agggccgcgc gccgcgcgtg ttgcgcacca
    37681 aggaggtcgg catcgaaatt cgccccaacg attgcctcga agtgcgctcc tccggcggcg
    37741 gcggctgggg accgccggcg cggcgatcgc cggccgcgcg tcaacgcgac cgcgaacagg
    37801 ggcttacgca agacagcgcg acgatggggc agggcggccg gtgatgttca cgatcggaat
    37861 tgatgtcggc gggacctaca ccgatctggt tgccatcgac gagcgtggca aagccgtgtt
    37921 cgcgaaatcg ccgtcgacgc catcagatca gtcggtcggc gtcatggccg ggctggaaga
    37981 gctggcgcgg cgattgcaaa tcacgcgcgc ggaaatgctt ggcgtgacgg accgcgtggt
    38041 tcacggaacc acggtcgcga ccaacgcctt gctggagcgg aagggcgctc atgtcgcgct
    38101 gctcacgacc gaaggccatc gcgatgtcat tgagatgcgc gagggtctca aaggcgaccg
    38161 ctacgatctg cgctcgccgc cgccggagcc gctggtgccg cgcgagcggc gctttggcgt
    38221 gaaggaacgg ctcaaggcca atggcgagat cttgacgccg ctcgacgatc gctcgctcgg
    38281 cgatgccatc tcagcgatca gcgcgtctga cgccacatcg gtcgcggtgt gctttctgca
    38341 ttcctatctc gacccggtcc acgaaatcgc cgccgtcgaa cgcctcgcgc gcggactgcc
    38401 cggtatcagc atttccaggt cgagcgatgt gctgccgcag atcaaggaat atgagcgggt
    38461 ctcgaccacg atcgtgaacg cctatgtcgg gccgctggtg cggcactatc tgaccaatct
    38521 ggagcagcgt ctcaaggggg cgggcttcaa gggcagtctc ttcatcgttc tgtcgcatgg
    38581 cggcatggcg ccggtggaag aagcctcgcg gcttgccgcg ggcacggtgc tgtcgggtcc
    38641 cgccggcggc gtttcgggcg cgcggcgctg cgccgatctg ctcggcattt ccgatctggt
    38701 tccgttcgat atgggcggca ccagtaccga catctcgctg atttcggaag gccgcgcgtc
    38761 gctatcggcg gacggaatgc tcgccgggca gcgcatcgcc ctgcgcagtc tcgacattgc
    38821 cagcatcgcg gccggcggcg gctcgatcgc cagcgtcgat gctggcggca cgttgcgcgt
    38881 cgggcccgag agcgcgggct cggtgccggg gccggcctgc tatggcagtg gcggccttgc
    38941 cgcgaccgtc accgatgcca atgtcgttct cggctatctc gacgccgccg ccttcatggg
    39001 cgggcagcgc ccgctcgatc gcgctgcgtc ggaagcggcg gtcgaccggg tcgcggtgtc
    39061 tttgggtctg tcgtggctcg aagccgccgc gggcatctac cgcatgatca atctgaagat
    39121 ggcggatggc atccggctga tgacgttgcg ccgcggtgtc gatccccggc ggtttgcgct
    39181 gttgagtttt ggcggcgcgg ccggcctgca tgccggcgaa gtcgcgcgcg aactggatat
    39241 caagcgcatc atcgtgccga cggcagcctc ggtgctgtcg gcatggggca tgctgaccag
    39301 cgacttgcgc tacgaagtca gccgcacgca ttacggcgcc ggcccgcgca tttcggcgga
    39361 tgaggcgcgc gcgctgtttt gtcggttgga gcagcaggcg gccggccggc ttcgcgcctg
    39421 gttcgacggt cccatgagca tcgaacgctc cgccgaaatg cgttatggcg agcagatctt
    39481 cgagatcgat gttgcgctcg acgatctcga ctgggacgcg gcctcgctgg tcgatcaaat
    39541 cgaagaccgc tttcatcgcc gtcacgagga gctgtacacc tattcctcgc gcgatcagga
    39601 ggtggtgttc gtcaacgcgc gcgtcgcagc aatcggcgaa gtgtcacggc gcggacaaga
    39661 cgagcggccg gcgccatcgg ctgccgcctg cgcaccgcgc accacgcggc aggcgttttt
    39721 cggcgcgtgg cgcgaggttc cggtttatgc gctggatgcg ctccagccgg gccacgcctt
    39781 cgaaggtccc gcaatcgtcg aggccgagac cacgacggtc atcgtcggcg ccggcgaccg
    39841 ggtgaccgtg aacgcgctcg gctggctgga tattgcgttg aggtgatgcg gtaagatggg
    39901 ggctgcgcgc cccgagctga catcgaatct tgcgctcatg actaatggca ctataccaac
    39961 tgcagccgca gctctcgccc cacgatttta gcggcgcgct gtaggctctc gatcgtggcg
    40021 ctgccgttgt cagggtccaa caagcggtcc acttgtgcgc ggctggtttt catcaactcg
    40081 gccatccgct gcttggtgat tttctttttc ttcatttcgc tggccagttg acgtgcgatc
    40141 acagccttga tcgccgcggc agttgcttcc tctcggattc ctgcgtcttc gagccagctt
    40201 tcgaagctcg aacctacgtg cggatttttc tttgccatag ctgcaactcc ttaaatctct
    40261 gccttgccaa cgtcagatct tcggtcgggg tcttctgtgt tttcttgatc atcgcgtgca
    40321 gagcgaccag tatgccgtcg tgaaacccga agaatatgcg cgcctcgcgc ttgctcggca
    40381 gcgaagcacg cacctcccac aagccgccac tcagcgcacg gcacaatggc aagcccaccg
    40441 gccacccgta ctgcaccttg gcgatatcgc gcccgatggt tcgcttatca tcgcgggata
    40501 gttcgttgag ccattcccgc accggctccc gacctgtcgc cgatcgccaa aacatgagag
    40561 ggatgggctt gaagctttca ggcattcact gtaccaaaaa agatgcataa atgcaacgtg
    40621 gaatagtccg agaaagtcgc gcttcggcgc gctttctggt tttcagattg ccgccaggaa
    40681 acttgccacc caaaatagct tcgctatatc ggatttttcc ataattttcc gccccgccat
    40741 caagtgagac ttacaatgac cgccactacc gacaccgccg cccccgaatc tcagccgttc
    40801 caggccgagg tggccgagct cctgcacctg atggtgcatt cggtctattc ggaaaccgat
    40861 attttcctgc gcgagctgat ttcgaatgcg tcggatgcct gcgacaagct gcgctacgag
    40921 gccatcgccg cgcccgatct gatcgccgac ggggagccgc ccaaaatcct gatcgcgccc
    40981 gacaagaagg ccgatacgtt tagcgtgatc gacagcggca tcgggatgga ccggcaggag
    41041 ctgatcgaca atcttggcac catcgcgcgc tccggcacca gatcgtttct gtcgcgcctg
    41101 accgaggcca aggatggcgc cggcctgatc ggccagttcg gcgtcgggtt ttactccgcc
    41161 ttcatggtcg ccgaccgcat cgtcgtcacc agccgccgcg ccggagcgga tcaggtctgg
    41221 acctggtcgt cgtcgggcgg cagcggattt gaaatcgcgc ccgccagcga ggacgacgca
    41281 aagcgcatca tgcgcggaac cgagatcgtg cttcacttaa aacccgacgc aaagaaatat
    41341 ctcgagatat acgagatcga gcgtgtcgtc ggcgcctatt ccgacaatat ccagtttccg
    41401 attctgctta aatcggagga cggcgagccg cgccagatca attcggcgag cgcgctgtgg
    41461 cagcggccga aatcggaact gacgccggag gattacaagc aggcctacaa gtcgatcgcc
    41521 ggcgccttcg atgaaccggc gatgacgctg cattaccgcg ccgaaggccg ctattcctac
    41581 gcggtgctat tgttcgcgcc atcgacaaaa ccgttcgatc tgttcgagcc gtcgcgcaag
    41641 ggaagggtaa agctctatgt ccgccgggta ttcattaccg acgatgccga tctgttgccg
    41701 gcgtatttgc gcttcatccg cggcgtcgtc gacagcgagg atctgccgct caacatctcg
    41761 cgcgaaatgc tgcagaacaa tccgcagctg gcgcagatca gaaaagcggt gaccagccgc
    41821 gtgatcaccg agctggagca gcttggcgag aaggagccgg aaaattttgc aaaaatctgg
    41881 gacgcctttg gccccgtgat caaggaaggc ctctatgagg actatgagcg gcgcgagaaa
    41941 ctgctggcgc tgtcgcgctt caccaccacc gcgggcgaga agcgctcgct gaagcaatat
    42001 gtcgcggacc tgaaacccaa tcagaccgag gtctactttc tggccggcga cagcatcgag
    42061 cggctgaaat ccaatcccaa gctggagcag gcggccgccc gcggcatcga
//
LOCUS       DASWGM010000790         2699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1314311,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000790 DASWGM010000000
VERSION     DASWGM010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314311"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..346
                     /locus_tag="VGA15_21900"
     CDS             44..346
                     /locus_tag="VGA15_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590385.1"
                     /translation="MKKRKFSRRDVLQGSLKGANGIFKAAPNPNAAKLFQNYCFTPEA
                     QQIIIDAGGLRSLHAQVKEHPGRKPLADIKLMKEAAEGAEKQSEEIKARYQKLFHV"
     gene            365..1426
                     /locus_tag="VGA15_21905"
     CDS             365..1426
                     /locus_tag="VGA15_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX9590386.1"
                     /translation="MRKRISRRDLIKGSAALGLTAFAAPLKAAAPEPVAITHDLVEAA
                     RKEAKVILYSSMDLPVGEKLGKAFEAQYPGIAVQIERSGSERLFQRLDQEFASGIRAA
                     DIVNSSDASHFISWKKNGWLAPFVTEDIAQHFLPEYRDPDGLFATSRIWLSSIAYNTN
                     LVKPDDAPKSFADLLDPKWAGKLVKGHPAYSGTIMTATFQMVRELGWDYMEKLSKQRV
                     MQVQSSTDPPKKLSLGERAVMADGNEYGIVLLKEAGQPVEPVYPIEGTPTISGPTGIF
                     VTAPHPNAARLFQAWLHTRETQQFFIDFTAQYSVHAQVPAKPGRRKISDIKLMKEDAA
                     GVEKMTEEIKTRYAKLFRV"
     gene            1644..>2699
                     /locus_tag="VGA15_21910"
     CDS             1644..>2699
                     /locus_tag="VGA15_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9590387.1"
                     /translation="MTITASLDATKGKIDWTKPVLALFAVCLILLIVLPLSWLAVYSV
                     TDKANHLTLQNFVTLFTDPDFLDPLLTTAIIATTSATICCLVAAPMGWLVSRTDMPGR
                     QFIRALVTASFVTPPFLGAVAWELLAAPNSGLLNQLYRFVTGAESDDHLFNIYSMAGI
                     IFVISCYTFPFVFVLVANALDNMPGELEDASAILGGKAWTTARRVTIPLALPALVAGA
                     LIAFLQAMTLFGSPAILALPAGFHTMTTKIWSLFQYPPKLELAAAAAVPLLLLTILLL
                     QAQKFILGRRGYSVVGGKYGAPRRVEMKKWRWAALAFCLVVLLNPVFLPYLALLNGAL
                     SPNATTLVTPSTLTMHNI"
BASE COUNT          531 a          852 c          805 g          511 t
ORIGIN      
        1 ttgccgggcg ctgagccgat aacaaaacca gcgggaggac gcgatgaaaa agcgaaaatt
       61 ttccaggcgc gacgttctgc aaggttcgct gaaaggcgcg aacggcatct tcaaggcggc
      121 gccgaatccc aatgcggcaa aactgttcca gaattactgc ttcacgccgg aagcgcagca
      181 gatcatcatc gacgccggcg gcctgcgttc gctgcatgcg caggtgaagg aacatccggg
      241 ccgcaagccg ctcgccgaca tcaagctgat gaaggaagcc gccgaaggtg cggagaaaca
      301 aagcgaagag atcaaggcac gttatcaaaa actcttccac gtctgacgca ggaaggggag
      361 accgatgcga aagagaattt cccggcgtga cctcatcaag ggctcggcag cgctcgggct
      421 caccgcgttc gccgcgccgc tgaaggccgc ggcacccgag ccggtggcga tcacgcatga
      481 cctggtcgag gccgcgcgca aagaggcaaa agtgattctt tattcgtcga tggatcttcc
      541 agtcggcgag aagctcggca aggcgttcga ggcgcagtat cccggcattg ctgtgcagat
      601 cgagcgctcc ggctcggagc ggctgttcca gcgcctggat caggaatttg cctccggcat
      661 ccgcgcagcc gacatcgtca acagctcgga cgcttcgcac ttcatctcct ggaagaaaaa
      721 cggctggctg gcgccgttcg tcaccgaaga catcgcgcag cattttttgc cggaatatcg
      781 cgaccccgac ggcctgttcg cgacctcgcg gatctggctg tcgtcgatcg cctacaatac
      841 caatctggta aagccggacg acgcgccgaa aagttttgcg gatttgctcg atccgaaatg
      901 ggccggcaaa ttggtaaagg gccatcccgc ctatagcggc accatcatga ccgcgacctt
      961 ccagatggtt cgcgagctcg gctgggacta catggagaag ctgtcgaagc agcgcgtgat
     1021 gcaggtgcag tcgtcgaccg atccaccgaa aaaactgtcg ctcggcgaac gcgccgtgat
     1081 ggccgacggc aacgaatacg gcatcgtgct gctcaaggaa gccggacaac ccgtcgagcc
     1141 ggtgtatccg atcgagggca cgccgacgat ctcgggtccg accggaattt ttgtcaccgc
     1201 gccgcatccc aacgccgcac ggctgttcca ggcctggctg cacacccgcg agacccagca
     1261 gtttttcatc gacttcaccg cgcagtactc ggtacacgcg caggtgccgg cgaagccggg
     1321 acgccgcaag atttccgaca tcaagctgat gaaggaagat gccgcaggcg tcgagaaaat
     1381 gacggaagaa atcaagacgc gctacgcgaa actgtttcgg gtgtgacgca tggagagaag
     1441 attcgtcggc gacttgcttt cacctcgccc cgcgcgcggg gagaggtcgg tgcgcatcgc
     1501 cagatgcgcg atgggtgagg gggcgtttct ccacgcccga gcctgttgaa acgccccctc
     1561 accccaaccc tctccccgcg cgcggggcga gggggattgc cgtgctcgct gcaagtgcaa
     1621 atccctcaag attggcaaac aaaatgacca tcaccgctag cctcgacgcc accaaaggca
     1681 agatcgactg gaccaagccg gtgctggcgc tgtttgcggt gtgcctgatc ctgctgatcg
     1741 tgctgccgct gtcgtggctc gcggtctaca gcgtcaccga caaggccaat catctgacct
     1801 tgcagaattt cgtcacgttg ttcaccgatc cggatttcct cgatccgctg ctgaccaccg
     1861 ccattatcgc caccacctcc gctacgatct gctgtctggt tgcggcgccc atgggatggc
     1921 tggtgtcgcg caccgatatg ccggggcggc aattcatccg cgcgctggtc acggcctcgt
     1981 tcgtgacgcc gccatttctc ggtgcggtgg cctgggaatt gctcgcggcg cccaacagcg
     2041 gattgctcaa ccagctctat cgctttgtca ccggcgcgga atccgacgat cacctgttca
     2101 atatctactc gatggccgga atcatctttg tcatctcctg ctatacgttt ccgtttgtgt
     2161 tcgttctcgt cgccaacgca ctggataaca tgcccggcga actcgaagat gcttcggcca
     2221 ttctcggcgg caaagcctgg accacggcgc ggcgcgtcac tatcccgctg gcgctccctg
     2281 cgctggtcgc cggcgcgttg atcgccttcc tgcaggccat gacgctgttc ggttcgcccg
     2341 cgatcctggc gctgccggcc ggcttccaca ccatgaccac gaagatctgg agcctgttcc
     2401 agtatccgcc aaaactcgaa cttgccgccg ccgccgcggt gccgctgttg ctcttgacta
     2461 tcctgctgct acaggcccaa aagttcattc tgggccggcg cggctattcg gtggtcggcg
     2521 gcaaatatgg cgcgccacgc cgcgtcgaaa tgaagaagtg gcgctgggcg gcgttggcgt
     2581 tctgcctcgt ggtgctgctc aatccggtgt tcctgccgta tctggcgctg ctgaacggcg
     2641 cgctctcgcc gaacgccaca acgctggtca cgccgtcgac gttgacgatg cataacatc
//
LOCUS       DASWGM010000791         8280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1592066,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000791 DASWGM010000000
VERSION     DASWGM010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592066"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VGA15_21915"
     CDS             complement(<1..118)
                     /locus_tag="VGA15_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HEX9590388.1"
                     /translation="MPWKNQGGGPWGSGPKGPWGSGPQSVGPRPPDLEDLLRR"
     gene            complement(288..800)
                     /locus_tag="VGA15_21920"
     CDS             complement(288..800)
                     /locus_tag="VGA15_21920"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX9590389.1"
                     /translation="MEVVLIVAVADNGVIGSGGTIPWRLKADQQRFKAMTMGKPVVMG
                     RKTFMSLRRPLPGRTNIVVTRDASFRSPGAVVTTSFADARAVATGDALRRLATEIAVI
                     GGAEIYAQWMDIADRLEVTEVHARPEGDTHLAAVDPAGWEEVARVGNSAGPDDSADYS
                     YVTYRRRKSR"
     gene            complement(800..1285)
                     /locus_tag="VGA15_21925"
     CDS             complement(800..1285)
                     /locus_tag="VGA15_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9590390.1"
                     /translation="MSVIIRRARPDEAGLVLSLVRELAEYEKLLHEVEATEAGIGEAL
                     FGSHPRLFCEIAEWKGEPAGFAVWFINFSTFSGRSGIYLEDLFVRPPHRGKGIGKALL
                     AHLARQCVANGWSQLQWAVLDWNTPSVEFYKSLGAVLMEEWTGCRIGGPALTALAQGT
                     R"
     gene            complement(1294..2088)
                     /locus_tag="VGA15_21930"
     CDS             complement(1294..2088)
                     /locus_tag="VGA15_21930"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX9590391.1"
                     /translation="MNQYHDLLERILGDGVEKHDRTGTGTLSIFGHQMRFNLAAGFPM
                     LTTKKLPLKSIVHELLWFLAGDTNIKYLRDNGVSIWNEWADANGDLGPVYGSQWRSWP
                     APDGRSIDQISNVIDMIRRNPDSRRLIVSAWNPADVDKMALPPCHCLFQFYVAAGKLS
                     CQLYQRSADVFLGVPFNIASYALLTMMVAQVTGLKPGDFVHSFGDVHLYSNHLEQARL
                     QLTRPTRALPVMKLNPDVKDIFAFRYEDFVLEGYEPHPHIKAEVAV"
     gene            complement(2279..3844)
                     /locus_tag="VGA15_21935"
     CDS             complement(2279..3844)
                     /locus_tag="VGA15_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX9590392.1"
                     /translation="MTDLFSNLALGFHVVFQFVPWSPAWLGGVSIPIPANILLCLIGA
                     LVGTLVGVLPGIGTIATVAMLLPITFGLPPVGALIMLAGIYYGAQYGGSTTSILVNIP
                     GEATSVVTTLDGFQMAKQGRAGPALAIAAIGSFFAGCVSTVLIAVLGAPLTKLALAFG
                     PAEYFSLMVLGLIFATVLAKGSILKAIAMIVFGLLLSMVGSDIETGASRMAFNIPELA
                     DGLGFATVAMGVFGFAEIIRNLDAGAEMDRDLVQQKITGLMPTKKDMIDSAPAIVRGT
                     ILGSILGILPGGGAVIASFAAYTFEKKIAKDPSRFGRGAIEGVAAPESANNAAAQTSF
                     IPLLTLGIPPNAVMALMVGAMTIHGIVPGPQVMQKQPDLVWGMIASMWVGNLMLIIIN
                     LPLVGIWVRLLRVPYRLMFPSIVIFCAIGIYSINNAPVDVILAGAFGLLGYWLIKHDF
                     EPAPLLLGMVLGPLMEENLRRALLISRGDASVFFTRPLSAVLMATAAFLLVLALLPTL
                     RKKRDEVFVESET"
     gene            complement(3851..4351)
                     /locus_tag="VGA15_21940"
     CDS             complement(3851..4351)
                     /locus_tag="VGA15_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX9590393.1"
                     /translation="MSDTPSHAPVRSIMPKWVRGPQDFVGGIALMAIAVFALWASSDL
                     QGMHGFSFGAGTVPRMFAGLLLGLGVAVTVVGLVTEGPHLATYAWRGPLFVSLSILSF
                     AITIRPLGLVISAFLTFMVAALGTHETRWKETTIVGIVLTLFCSFLFPYALGLPMQLW
                     PRFLVQ"
     gene            complement(4440..4973)
                     /locus_tag="VGA15_21945"
     CDS             complement(4440..4973)
                     /locus_tag="VGA15_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX9590394.1"
                     /translation="MNSDTNPILSLAWTFGVMSLFAVGGANAAIPEMHRVAVDVQHWM
                     TDQQFADVFAISQLSPGPNVLIVTLIGYSVAGVAGALVATLAMCGPTAILAYYVSRLM
                     TRSSHSRWPAIIQVALVPLSIGLMGASGLILALAADRSWAGGVVTAVAAVLAFATRLN
                     PLWLLLAGGCLGFAGVI"
     gene            complement(4970..5581)
                     /locus_tag="VGA15_21950"
     CDS             complement(4970..5581)
                     /locus_tag="VGA15_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX9590395.1"
                     /translation="MPPDSPPVAVPAPDIPELAPSTPPGLVALFVAFAKMSLAGFGGV
                     LVWARRGIVDQHRWMTADEFNETFALCHFLPGPNVVNLSVVFGSRFRGIPGGLAAFAG
                     LLGPPVVIVTVLAALYARYGEIDALRRILAGVSCAAVGLLISALFRMMMPLIKRRDLI
                     GLVILAAVFVAIGLLRLPLPAVLLVAIPLSIAIAYFIRRRATS"
     gene            5692..6048
                     /gene="ssrA"
                     /locus_tag="VGA15_21955"
     tmRNA           5692..6048
                     /gene="ssrA"
                     /locus_tag="VGA15_21955"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            6233..6754
                     /locus_tag="VGA15_21960"
     CDS             6233..6754
                     /locus_tag="VGA15_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX9590396.1"
                     /translation="MATDHIRYDVLARDALRGVLRRVLTDAAEHGLPGEHHFFITFLS
                     TAEGVKLSPRLLAQYPEEMTVILQHQFWDLTVTEDRFEVGLSFGGIAERLVVPFNSIK
                     SFFDPSVQFGLQFEPNDAAAEAPAASLPSAAAPSSTASALTAPAPTAENKDEPAKPGE
                     GAEVVRLDRFRKK"
     gene            6853..8271
                     /gene="fumC"
                     /locus_tag="VGA15_21965"
     CDS             6853..8271
                     /gene="fumC"
                     /locus_tag="VGA15_21965"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX9590397.1"
                     /translation="MDRSAAPSRSNSTRSETDSFGPIEVPADRYWGAQTERSRRNFRI
                     GQDRMPMPIVHALGIVKLAAAETNRELALLDQRRARAIARAAREVIDGKLDDHFPLVV
                     WQTGSGTQTNMNLNEVIANRANELLGGELGAKTPVHPNDHVNMSQSSNDSFPTAMHIA
                     AATGIVGDLIPALSELHRALRKKEKAFAHIVKIGRTHTQDATPLTLGQEFSGYAAQVE
                     SGIARLRVAVKDLYPLAQGGTAVGTGLNSKPKFAKAFARHVAKITKLPFTSAPNKFEA
                     LASNDAYVFAHGAINSVATGLFKIANDIRLLGSGPRSGLGELILPENEPGSSIMPGKV
                     NPTQCEAVTMVCCQVFGNHTAITIAGSQGHFELNVYKPVLAYCMMHSIQLMSDVARSF
                     TEHCVEGIRADEKRIRELMERSLMLVTALAPRIGYDNAAKVAKSAHARGTTLKEEAVR
                     LGFVSAAEFDRLVQPAKMTHPG"
BASE COUNT         1751 a         2686 c         2480 g         1363 t
ORIGIN      
        1 cacggcgcag aaggtcttca agatcgggcg gcctcggccc caccgattgc gggccggagc
       61 cccacggccc ttttggaccc gagccccatg ggcctccgcc ttgattcttc cacggcatcg
      121 acagtctcct ctgcgggctc aaagggccaa aacgcccagc ctcgcaccat ctgcacggcc
      181 ttatagggga ccgccccggc ccttacaacg cagcttgttg gcaggttctg gcccgaagga
      241 gctaagcccg aacccgtaat tgtcaatgta aacatcgcct gacggggtta acgcgacttt
      301 cgtcggcgat atgtcacata ggaatagtcc gcgctgtcgt ccggaccggc cgaatttcca
      361 acgcgcgcaa cctcttccca gcccgccgga tcgaccgcgg caaggtgagt atcgccctcg
      421 ggccgggcgt gaacttcggt aacttccagg cggtcggcga tatccatcca ctgcgcgtag
      481 atctcggcac cgccgataac ggcgatttca gtggctaaac gccgcagcgc gtcgccggtc
      541 gcgaccgcgc gggcatcggc gaacgacgtc gtcaccaccg cccccgggct gcgaaaactg
      601 gcatcgcggg tgacgacgat attggtgcgt ccgggaagcg gccggcgcag cgacatgaag
      661 gtcttgcgac ccatcaccac cggcttgccc atcgtcatcg ccttgaagcg ctgctggtcg
      721 gctttcagcc gccatgggat cgtgccgcca gaaccgatca cgccgttgtc ggcgaccgcg
      781 acaatgagga cgacctccat caacgcgttc cctgcgccag cgcggtgagc gccggaccgc
      841 cgatccggca cccggtccac tcctccatca agaccgcgcc gagcgatttg tagaattcga
      901 ccgacggcgt gttccagtcc agcaccgccc attgcaattg cgaccagcca ttcgccacgc
      961 actgcctggc gagatgggcc agcagtgcct tgccgatgcc tttgccgcgg tgcggcggac
     1021 gcacgaacag gtcttccaga tagataccgg agcggccgct gaaggtcgaa aaattgatga
     1081 accagaccgc gaaaccggcc ggctcgccct tccattccgc gatctcgcag aacagccgtg
     1141 gatggctgcc gaacagcgcc tcgccgatgc cagcttcggt ggcctcgact tcatgcagca
     1201 gcttttcgta ctcggcgagt tcgcgaacca gcgacagaac cagtccggct tcgtcgggac
     1261 gcgcgcggcg gataatgacg gacattcaat cgctcacacc gcgacctcgg ccttgatgtg
     1321 cgggtgcggt tcgtaaccct cgagcacgaa gtcttcatag cggaacgcaa agatatcctt
     1381 cacatcggga ttgagcttca tcaccggcag cgcgcgggtc gggcgcgtca gctgcagccg
     1441 ggcctgctcg aggtggttcg aatagaggtg aacgtcgccg aatgagtgaa cgaagtcgcc
     1501 gggcttgagg cccgtcacct gcgccaccat catcgtcagc aacgcatagg aagcgatgtt
     1561 gaagggcacg ccgagaaaca cgtccgccga acgctgatag agctggcacg ataatttgcc
     1621 ggctgcgacg tagaactgaa acagacaatg acacggcggc aacgccatct tgtcgacatc
     1681 ggccggattc cacgcgctca cgatcaggcg gcgcgaatcc ggattacgcc ggatcatgtc
     1741 gatgacgttg gaaatctggt cgatgctgcg tccgtccggc gccggccagg atcgccactg
     1801 cgatccatag accgggccga gatcgccatt ggcgtcagcc cattcgttcc agatcgaaac
     1861 gccgttatcc ctgagatatt taatgttggt atcgccggcc agaaaccaca acagctcgtg
     1921 cacgatcgat ttgagcggca gcttcttggt ggtcagcatg gggaatccgg cggccagatt
     1981 gaagcgcatc tgatggccga agatcgacaa ggtgccggtg ccggtgcggt cgtgtttttc
     2041 cacgccgtcg ccgagaatcc gttcgagcag atcgtgatat tgattcatgg cgttgcgctg
     2101 cggtcctcgg aaaacgcgaa ttttagcggc cggaccggcc aatcgacagt ggcgactcgg
     2161 ccggatgcat cgattatacc cgaaaaatgc gcatccggac cacaaacgac aagggcagaa
     2221 cctgcattct gcccttgtcc gcggccggtt ccgtcagccg acggcgtacc gacgcaggtc
     2281 aggtctccga ctccacaaac acctcgtcgc gcttcttgcg cagcgtcggc aacaacgcca
     2341 atacgagcag aaatgcagcg gtcgccatca gcaccgccga gagcggccgg gtgaagaaca
     2401 cgcttgcatc gccgcgcgag atcaacagcg cccggcgcag attttcctcc atcagcggtc
     2461 cgagcaccat cccgagcagc agcggcgccg gttcgaaatc gtgcttgatc agccaatagc
     2521 cgagcagtcc gaacgcgcct gcgagtatga cgtccacggg ggcgttgttg atggaataga
     2581 tgccgatcgc gcagaagatc acgatcgatg gaaacatcag gcgatacggc acccgcagca
     2641 accgcaccca gatgccgacc agcggcaggt tgatgatgat cagcatcaga ttgccgaccc
     2701 acatcgaggc gatcatgccc cacaccagat ctggctgctt ctgcatcacc tgcggtcccg
     2761 gcacgatgcc gtgaatggtc atcgcgccga ccatcagggc catcaccgca ttgggcggaa
     2821 tgccgagcgt caacagcggg atgaacgagg tctgcgccgc cgcgttattg gcgctttccg
     2881 gcgccgcgac gccttcgatc gcgccgcggc caaaccgcga cggatccttg gcgatcttct
     2941 tctcgaacgt ataggccgcg aacgaggcga tcaccgcgcc gcctcccggc agaataccga
     3001 ggatcgagcc caggatcgtg ccgcggacga tggccggcgc agaatcgatc atatcctttt
     3061 tggtcggcat cagcccggtg atcttctgct gcacgagatc gcggtccatc tcggcgccgg
     3121 catccagatt gcggatgatc tccgcgaatc cgaacacgcc catcgccacc gtggcaaagc
     3181 cgagaccatc ggcgagttcg ggaatgttga acgccatccg cgaggcgccg gtctcgatgt
     3241 cggagccgac catcgacagc aacagcccga acacaatcat cgcaatggct ttcaggatcg
     3301 atcccttagc cagcacggtc gcgaagatca ggcccagcac catcagcgag aaatattcgg
     3361 ccggtccgaa cgccagcgcg agcttggtca gcggcgcgcc cagaacggcg atcagcacgg
     3421 tcgagacgca gccggcgaaa aacgacccga tcgcggcgat cgcgagcgcc gggccggcgc
     3481 ggccttgttt ggccatctgg aagccatcga gcgtcgtcac caccgacgtc gcctcgccgg
     3541 gaatattgac caggatcgag gtggtcgaac cgccatattg cgcgccgtaa taaatgcccg
     3601 cgagcatgat cagcgcaccg accggcggca gaccgaaagt gatcggcagc agcatggcga
     3661 cggtcgcgat agtgccgatg cccggcagca cgccgaccag cgttccgacc agcgcgccga
     3721 tcagacagag caatatgttg gccgggatcg ggatcgagac gccaccgagc cacgctggcg
     3781 accacggcac aaactggaat acgacatgga agccgagcgc gagattggaa aagagatcgg
     3841 tcatcgtccc tcactgaacc aggaagcggg gccagagctg catcggcagc cctaacgcgt
     3901 aaggaaaaag aaaactgcag aatagggtca atacgatgcc aacgatggtg gtttccttcc
     3961 accttgtctc gtgcgttccc agcgcggcaa ccatgaaggt gaggaacgcc gaaatcacca
     4021 gacccagcgg gcggatcgtg atggcaaacg aaagaatcga cagcgagacg aacagcggcc
     4081 cccgccaggc ataggtcgcc aaatgcgggc cttcggtcac cagaccgacc accgtcaccg
     4141 cgacccccaa cccgagcagc aggcccgcaa acatgcgtgg cacggtgccg gctccgaacg
     4201 aaaatccatg catgccctgc agatcgctcg acgcccacaa ggcgaacacc gcgatcgcca
     4261 tcagcgctat tccgccaacg aaatcctgcg ggccacggac ccatttcggc ataatggacc
     4321 tgaccggcgc atggctcggc gtatcactca tcgatctctc tccccgcgca gggcttctcg
     4381 cccattctgt ttggatagcg ttgatgctgc tccgaccggt tcctgcctac cgattttcct
     4441 cagataacgc cagcaaaacc caggcagcct cccgcaagca acagccatag cggattgagc
     4501 cgcgtggcaa aggccagcac cgcggcgaca gcggtgacca cgccgccggc ccagctccga
     4561 tccgcggcca gcgccaaaat caagccgctg gcgcccatca atccgattga gagcggaacg
     4621 agggccacct gaataatcgc gggccagcgc gagtggctcg atcgcgtcat caggcggctc
     4681 acataatagg caaggatagc cgtcggaccg cacatcgcca gggttgcaac cagcgcgcct
     4741 gcaacgccgg cgacggaata gccgatcagg gtcacaatca gcacattcgg ccccggcgac
     4801 aattgcgata tcgcgaacac gtcggcgaat tgctggtcgg tcatccagtg ctgaacatcg
     4861 accgcaacgc gatgcatttc gggaatggcg gcattggcgc cccccaccgc gaacagcgac
     4921 atcaccccga aagtccaggc gagcgacaga attgggttgg tgtctgaatt catgaggtag
     4981 cccgccggcg gatgaaataa gcgatggcga tgctgagcgg gattgccacc agcaataccg
     5041 ccggcaacgg cagccgcagc agcccgatcg cgacaaaaac cgccgccagg atcacaaggc
     5101 cgatcaggtc gcgccgcttg atcaacggca tcatcatccg gaacaaggcc gagatcagca
     5161 atccgaccgc agcacaggag accccggcca aaatgcgccg caacgcatcg atttcgccgt
     5221 agcgggcgta gagcgccgcc aggacggtca cgatcaccac gggcggaccg agcagcccgg
     5281 caaaggctgc gaggccgcca ggaatcccgc gaaaacgcga tccaaacacg accgacaggt
     5341 tcacgacgtt cgggccgggc agaaaatggc acagcgcgaa ggtctcgttg aactcgtccg
     5401 ccgtcatcca tcggtgctga tcgacgatgc cgcgccgcgc ccagaccagc acgccgccaa
     5461 agccggcgag cgacattttg gcgaacgcga cgaacagcgc gaccaggccg ggcggcgttg
     5521 acggggctaa ttctggaata tccggcgccg gaacggcaac cggcggcgaa tccgggggca
     5581 ttatcaagat ttagaacggc ggctgagcaa ggccaaccca tattcaacgc attccacttc
     5641 agcgtagtct accccgaggg gcgcggttcc ccggcgaaac ggccctcgca gaccctgttt
     5701 ttctcaactt ttgcccccta tattgagggg gccggcctgc cggctatgga aataaattgc
     5761 cgtcgcaata aaccattcgg acccgggggc agcacccggc gcctccacca aagtccgccg
     5821 ccaggctgcg ggtttcggcg ggggcgaaac aggatcgacg agggcgtaaa gggcgtgttt
     5881 tttctcggta tggttccgcc gttatcgggc tatgcaatag ttgcaaacga caactatgct
     5941 ccggttgctc aggctgcgta agcggtttga aagaccaagt ctgaagtcct gatgggttaa
     6001 gctccgtcag gcggggttcg gaggcacctg gcaacagaag cctccacttc aatttgaact
     6061 tgcctcatcc caggtttgcc ttatcccagg tcagattgct ggaatttgca ccactggcgg
     6121 caacacctgc tgacctcagg ccgccttcgg agtagcatgc acgaaagggg cgagtcgggc
     6181 tgccgatccg ccggcggcat ccgaacgtga caggaccgac acaggaccga caatggcgac
     6241 cgatcatatc cgatacgacg tcctggcgcg cgacgcgctg cggggtgtgc tgcgccgcgt
     6301 tctgaccgac gccgccgagc atggcctgcc tggcgaacat catttcttca tcacctttct
     6361 gtcgaccgcg gaaggcgtga agctgtcgcc gcggctgttg gcgcaatatc cggaagaaat
     6421 gacggtcatt ctccagcacc agttctggga cctgacggtg accgaagatc ggtttgaagt
     6481 cggcctctcg ttcggcggca tcgccgagcg cctggtggtt ccgttcaatt ccatcaagag
     6541 cttcttcgat ccgtcggtgc aattcggcct gcagttcgag ccgaacgatg ccgcggccga
     6601 agcgccggcg gcaagccttc cgtcggctgc tgcgccctct tccacagctt cggcgctgac
     6661 ggctcccgcc cctaccgccg agaacaagga cgagccggca aagccgggcg agggcgccga
     6721 agtcgtgcgg ctggatcgct ttcgcaagaa ataatccgcg cgcgagaaat ccgcgccgct
     6781 gtagaatgaa cgtccgcgga gccggcgtga gctggttccg aaacaaccgg ccttgcgtaa
     6841 cggacatgat tcatggatcg atcagcagca ccctcgcgat ccaattcgac gcgctccgag
     6901 actgacagct tcggcccgat cgaggttccc gccgatcgct actggggcgc gcagacggaa
     6961 cggtcgcggc ggaatttccg tatcggccag gaccggatgc cgatgccgat cgttcatgcg
     7021 ctcggcatcg tcaagctcgc ggcggcggaa accaatcgcg aactcgcctt gctcgaccag
     7081 cgtcgcgccc gcgccatcgc ccgcgccgcg cgcgaggtga tcgacggcaa gctcgacgat
     7141 cattttccgc tggtggtctg gcagaccggc tccggcacgc aaaccaacat gaacctcaac
     7201 gaggtgatcg ccaatcgcgc caacgaactg ttgggcggcg agctcggcgc caagacgccg
     7261 gttcatccga acgatcacgt caatatgagt cagtcgtcga acgactcatt tccgactgcg
     7321 atgcacatcg ccgcggcgac gggcatcgtc ggcgatctca ttccggcgct cagcgagctt
     7381 caccgcgcgc tgcgcaaaaa ggaaaaggcg ttcgctcaca tcgtcaagat cggccgcacc
     7441 catacccagg acgccacacc gctgacgctc ggtcaggagt tctccggcta cgccgcccag
     7501 gtcgagagcg gcatcgcgcg gctgcgcgtc gcggtgaagg atctgtatcc actggcgcag
     7561 ggcggcaccg ctgtcggtac cgggctcaat tcgaagccga aattcgccaa ggcgttcgcc
     7621 agacacgtcg ccaaaatcac aaagcttccc ttcaccagcg cccccaacaa attcgaggcg
     7681 ctggcctcca acgacgccta cgtattcgcg cacggcgcga tcaattcggt ggcgaccggc
     7741 ctgttcaaga tcgcgaacga tatccgcctg ttgggatcgg gtccgcgttc cggcctcggc
     7801 gaattgatcc tgcctgaaaa cgaacccggc tcgtcgatca tgccgggcaa ggtcaacccg
     7861 acgcaatgcg aagcggtgac catggtttgc tgtcaggtgt tcggcaacca tacggcgatc
     7921 accattgcgg gcagccaggg acatttcgag ctgaacgtct acaaaccggt gctcgcctat
     7981 tgcatgatgc attccatcca attgatgtcc gacgtcgcgc gctcgttcac ggagcattgt
     8041 gtcgaaggaa tacgcgccga cgaaaaacgt atccgggagc tgatggaacg ctcgctgatg
     8101 ctggtgaccg cgctcgctcc caggatcggc tacgacaatg ccgccaaggt cgccaagtca
     8161 gctcacgccc gcggaaccac gttgaaggaa gaggctgtgc ggctgggctt tgtctcggcg
     8221 gcggaattcg atcggctggt gcagcccgcc aaaatgacac atccgggctg atgccgcggc
//
LOCUS       DASWGM010000792         5487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2318165,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000792 DASWGM010000000
VERSION     DASWGM010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2318165"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VGA15_21970"
     CDS             <1..216
                     /locus_tag="VGA15_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9590398.1"
                     /translation="EEFDVEHLISGARVANPASRNILEKCGFQWSGVELHRFEALGSS
                     TPVDCFRLSRSVWSSLKNWNNSTRHER"
     gene            complement(577..1494)
                     /locus_tag="VGA15_21975"
     CDS             complement(577..1494)
                     /locus_tag="VGA15_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9590399.1"
                     /translation="MRLFSNISAYKDERSARLAGIGLMLLSIFMFSFGDAMGKFIVAT
                     YSVGQLLLLRGCAALIVLSPSIWRHRAQFMLLERPWLQLLRVALSTLEVAAFFLAAVY
                     LPLADVVTYYLACPIFVTALSAIVLRERVGWRRWTAVLIGFCGVLIALRPSTQTISWP
                     AVIALGGSLSFAVLMLITRSLRATPDIVLASSQFAGTFLLGALLSPFGWVTPGLVSLV
                     LFAAAGCISVAALLCVNRSLKLAPASVVVPYQYSMIIWAVMFGYFVFGDVPQVATILG
                     AAIIIGAGIYIFMRERALGRGETVVSPPV"
     gene            1779..2264
                     /locus_tag="VGA15_21980"
     CDS             1779..2264
                     /locus_tag="VGA15_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9590400.1"
                     /translation="MTDFDPALHRMVPQQRWFEDFALGERFVIPSRTMTSAVFAAFQT
                     ASGDTHPVHYDVEYCRVRGMPNLLAHGFQTLIHTAPGSGLFPYMVEESLVGFLEQSSR
                     FLKPVFADDTIYPALEVTELAPGRTTGVVALRSTVFNQRRELVLEGLQKFLIRRRPAQ
                     P"
     gene            2362..3411
                     /gene="obgE"
                     /locus_tag="VGA15_21985"
     CDS             2362..3411
                     /gene="obgE"
                     /locus_tag="VGA15_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX9590401.1"
                     /translation="MKFLDEAKVYIRSGDGGNGCVAFRREKYIEFGGPSGGNGGRGGD
                     VVIEVVDGLNTLIDYRYQQHFKAQKGTNGMGKDRHGANGKPIVLKVPVGTQIFDEDRE
                     TLIHDFTTLGEKFVLAEGGNGGFGNAHFKSSTNRAPRNANPGLEGEERWIWLRLKLIA
                     DAGLVGLPNAGKSTFLSVVSAAKPKIADYPFTTLHPQLGVVDVDGREFVLADIPGLIE
                     GAHEGAGLGDRFLGHVERCRVLLHLVDATCEHAGKAYKTVRTELDAYEGQLTDKIEIV
                     ALNKIDAVTPEQLKAQKDRLKRAAKKTPLLMSGVTGAGVKDALRALVEVIGEAPVSVK
                     AKGAAQAEPWAPQAT"
     gene            3705..4847
                     /gene="proB"
                     /locus_tag="VGA15_21990"
     CDS             3705..4847
                     /gene="proB"
                     /locus_tag="VGA15_21990"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX9590402.1"
                     /translation="MVRPELKNFRRIVVKVGSSLLIDSDAGEVRASWLAALAADLAKL
                     HGEGRELLVVSSGSIALGRSRLKLPRGVLKLEESQGAAAVGQIALARIWSEVLGHHGI
                     GAGQILVTLQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATTEIRYGDNDRLAA
                     RVATMTSADLLILLSDIDGLYDAPPAGDPNAKLIPIVETVTSQIEAMAGAAESELSRG
                     GMRTKIEAAKIATTSGTHMLIASGKIEHPLRAVADGGRCTWFLTPANPVTARKRWIAG
                     SLEPKGTLTIDAGAVSALRAGKSLLPAGVIRVDGQFARGDAVVVRGPDTHEIGRGLVA
                     YDADDAEKIKGRSSPDVMTILGISGRAEMIHRDDLVVGPSGASSAS"
     gene            5002..>5487
                     /gene="proA"
                     /locus_tag="VGA15_21995"
     CDS             5002..>5487
                     /gene="proA"
                     /locus_tag="VGA15_21995"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490592.1"
                     /note="Catalyzes the phosphorylation of L-glutamate during
                     the proline biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX9590403.1"
                     /translation="MSAPLKAIDGNADLPVLMAGLAIHARAAARVLALASPEQKNHAL
                     DAIERAIRANASAILGANAEDVAEVRAGGASDAFIDRLTLTQARIDAMAGGVATVRDI
                     TDPIGVVTESWQRPNGMIIERVRVPLGVVAVIFESRPNVAADAGVLCLKSGNAVILRG
                     GS"
BASE COUNT         1115 a         1751 c         1700 g          921 t
ORIGIN      
        1 gaggaattcg atgtcgaaca cctgatctcc ggcgcgcggg tcgccaaccc ggcgtcgcgc
       61 aacattctgg agaaatgcgg cttccagtgg agcggcgtcg aactgcaccg cttcgaggcg
      121 ctgggatcct ccacgccggt cgattgcttc cgcctttcgc gcagcgtctg gtcgtcgctg
      181 aaaaactgga acaattcgac gcgccatgag cggtgattta tcggtaacaa aagttggtcg
      241 tccccgcgaa aaacaacaga tcgtcgtccc cgcgaagccg gctgtgtgaa aactccgact
      301 tcgaactttc gtgtagaaat tctttctcga ttacgatgga ttggaaaaga actgctctgc
      361 cagtcatcac cgaaagaaga aaagggagaa aacaattctg cgcattctct gctcgcgcgc
      421 gtttttacac agcctcgaag gcggggaccc atagcccctg cggtatcgat tttataaaac
      481 gccggagcag ccattcggcg tgacaacaac gccggtggtt ataggtcccc gccttcgcgg
      541 ggaggacggc ggtgtttggc gttttgccac gcgccatcac accggcggac tgaccaccgt
      601 ctcgccgcgc cccagcgctc gctcgcgcat gaaaatatag atccccgcgc cgatgataat
      661 ggccgcgccc aaaatcgtcg caacctgtgg cacgtcgccg aacacgaaat agccgaacat
      721 cacggcccag atgatcatcg aatattggta cggcaccacc acgctggcag gcgcaagttt
      781 cagcgaacga ttgacgcaca atagcgccgc caccgaaatg caccccgccg cggcgaacag
      841 aacgaggctc acgaggcccg gcgtgaccca gccgaatggc gacaacagcg cgccgagcag
      901 gaacgtgccg gcgaattgcg acgacgccag cacgatatcg ggcgtcgccc gcagcgagcg
      961 ggtgatcagc atcaacaccg caaacgacag gctgccgccg agcgcgatga cagcgggcca
     1021 gcttatcgtc tgggtcgatg gacgcagcgc tatcagcacg ccgcagaagc cgatcaggac
     1081 cgcggtccag cgccgccagc cgacgcgctc gcgcagcacg atcgccgaca gcgcggtaac
     1141 gaaaatcgga caggccagat aataggtgac gacgtcagcg agcggcagat agacggccgc
     1201 cagaaaaaaa gccgcgactt cgagggtcga gagcgccacc cgaagcaatt gcagccacgg
     1261 ccgttcgagc agcatgaact gcgcgcgatg ccgccagatc gacggcgaca gcacgattag
     1321 cgccgcgcag ccgcgcaaca aaagcaattg accgaccgaa taggtcgcga cgatgaactt
     1381 tcccatcgca tcgccgaatg agaacatgaa gatcgacagc agcatcagtc cgatgccggc
     1441 gaggcgcgcg gaacgctcgt ccttataggc cgagatgttt gaaaacagcc tcatttgcgg
     1501 cgccgccttt atcttcgtca tggccggact tgttccggcc gtcttttgct tttgcgccgc
     1561 gctcttttaa agacgtggat gcccgggaca agcccgggca tgatgaattg gctaccagca
     1621 gcgttttcaa gcgaagtgga aaccggttcg cgtcaagaaa acgcgtcaaa tcaagaatct
     1681 ggaggctgtt tcgattctat cgaaacgggc tccagcagcg ttgccgcaac aacacctctg
     1741 cgatacgcat gggtcagacc cgacgcagga aacccgccat gaccgacttc gatcccgccc
     1801 tgcatcgcat ggtgccgcaa caacgctggt tcgaggattt tgcgctgggc gagcgttttg
     1861 tcattcccag ccgcaccatg acgtcggccg tgttcgccgc gtttcagacc gcgagcggcg
     1921 atacccatcc cgtgcattac gatgtcgaat attgccgcgt ccgcgggatg ccgaacctgc
     1981 tcgcgcatgg tttccagacg ctgatccata ccgcaccggg cagcgggctg ttcccctata
     2041 tggtggagga atcgctggtc gggtttctcg aacagtcgag ccggttcctg aaacccgtgt
     2101 tcgccgacga tacgatctat cccgcgctcg aggttacgga gctggcaccc ggccgcacta
     2161 ccggcgtggt ggcattgcgc agcaccgtgt tcaatcagcg ccgcgaactg gtgctggaag
     2221 gcctgcagaa gtttctcatt cgccggcggc ctgcccagcc ataacaggcg aagccgcaaa
     2281 atgcccatta attaaggctt tttgctaatt tcctcgcatc taatggttgc cgcaccggca
     2341 cccctgccct acttatgggc catgaaattc cttgatgaag caaaagtcta tatccgctcc
     2401 ggcgacggcg gcaacggctg cgtggcgttt cgccgcgaga agtacatcga gttcggcgga
     2461 ccgagcggcg gcaatggcgg ccgcggcggc gacgtcgtca tcgaagtggt cgatggattg
     2521 aacaccctga tcgactaccg ctatcagcag cacttcaagg cgcagaaggg caccaacggc
     2581 atgggcaagg accgccacgg cgccaacggc aagccgatcg tgctcaaagt gccggtcggc
     2641 actcagattt tcgacgagga ccgcgaaacc ctgatccacg atttcaccac gctcggcgag
     2701 aagttcgtgc tcgccgaagg cggcaatggc ggattcggca atgcgcattt caaatcgtcc
     2761 accaaccgcg cgccgcgcaa tgccaatccc ggtctggaag gcgaggagcg ctggatctgg
     2821 ctgcggctga aattgatcgc ggatgccggc ctagtcggcc tgcccaacgc tggcaagtcg
     2881 accttcctct ccgtcgtcag cgcggccaag ccgaagatcg ccgactatcc cttcaccacg
     2941 ctgcatccgc aactcggcgt ggtcgatgtc gatggccgcg aattcgtgct ggcggacatt
     3001 ccgggattga tcgaaggcgc gcatgaaggc gccggcctcg gcgaccggtt tttgggccat
     3061 gtcgagcgct gccgtgtctt gctgcatctg gtcgacgcca cctgcgagca cgctggcaag
     3121 gcctacaaga cggtgcgcac cgaacttgac gcctatgagg gacagcttac cgacaagatc
     3181 gagatcgtcg cgttgaacaa gatcgacgcg gtgacgccgg aacaattaaa ggcgcagaag
     3241 gaccggctga agcgcgccgc caagaagacc ccgctgctga tgtcgggcgt cacaggagcc
     3301 ggcgtgaagg acgcgctgcg ggcgctggtc gaggtgatcg gcgaagcccc ggtgtcggtg
     3361 aaggccaagg gcgcggcgca ggccgagccg tgggcgccgc aagccaccta gcgtgtgaaa
     3421 catggattgc aacggagcct gccgcgagtt cagtcccacc tctcccatgg ggagaggtcg
     3481 gatcgcatcg tatgcgatcc gggtgagggg ttaaggacta acgatggacc gtaccccctc
     3541 accccaactc tctcccgctc gaagtcggat atatccgact tcgacaattt gatataccca
     3601 actcgggtaa acccgagttg ggtgggagag ggagcgcgcc cccgttgctg caatcctttt
     3661 gtgttccacg tactctccga agcccagccg caaagtctca ttccatggtc cgcccagagc
     3721 tgaaaaactt ccgccgtatc gtcgtcaagg tcggctcgtc gctgctgatc gactcggatg
     3781 ccggcgaagt gcgggcgtcg tggctcgcag ccctcgccgc cgacctggca aaactgcatg
     3841 gcgaggggcg cgaactcctg gtcgtctcgt cgggttcgat cgcgctcggc cgcagccgtc
     3901 tgaagctgcc gcgcggcgtg ctgaagctgg aggaaagcca gggcgctgcc gcggtgggac
     3961 agatcgcact ggcgcggatc tggtcggagg tgctggggca tcacggcatc ggcgccgggc
     4021 agatcctggt gacgctgcag gacaccgagg agcgccgccg ttatctcaac gcccgctcga
     4081 ccatcgccaa gctgttggaa tggcgcgccg tgccggtgat caacgaaaac gacaccgtcg
     4141 ccaccactga aatccgctac ggcgacaatg atcgcctcgc cgcccgggtc gccaccatga
     4201 cgagcgcgga ccttctgatc ctgctttccg atatcgacgg cctctacgat gcaccgcccg
     4261 ccggcgatcc aaacgcaaaa ctgattccga tcgtcgaaac cgtcacatcg caaatcgagg
     4321 cgatggcggg cgccgcggaa tccgagctgt cgcgcggcgg catgcgcacc aagatcgagg
     4381 cggcgaagat cgccaccaca tcgggcacgc atatgctgat cgcatccggc aagatcgagc
     4441 acccgctgcg ggccgttgca gatggcgggc gctgcacctg gtttctgacg ccggccaatc
     4501 ccgtgaccgc gcggaagcga tggattgccg gctcattgga gccgaagggg acgctgacca
     4561 tcgatgccgg cgcggtgagc gcgttgcgcg ccggcaaaag cctgttgccg gccggcgtga
     4621 tccgggtcga cggacagttc gcccgcggcg atgccgtcgt ggtgcgcggc ccggacaccc
     4681 acgagatcgg gcgcggcctc gttgcctatg acgccgacga cgccgagaag atcaagggcc
     4741 gctcgtcgcc ggatgtgatg accatcctcg gcatctcggg acgcgccgag atgatccatc
     4801 gcgacgatct ggtggtcggc cccagcggcg cctcttccgc gagctaggcc aatggccgag
     4861 tggctgccaa gccggccgca cgccaaccca aaagagctgg caccgccccc agccatgccc
     4921 gccccgcacc tcgcaaaaag cggatttccg tgctaggata tggccttaac tgaaagctgg
     4981 gaccgaaaag ctgggatcga gatgagcgcg ccgctgaagg caatcgacgg caacgccgat
     5041 ttgccggtcc tgatggccgg ccttgctatc cacgcgcgcg ccgccgcgcg ggtgctggcg
     5101 cttgcctccc ccgagcagaa aaaccacgcg cttgatgcga tcgagcgcgc cattcgcgcc
     5161 aatgcatccg cgatccttgg cgccaacgcc gaagatgtgg cggaagttcg cgccggcggc
     5221 gccagcgacg ccttcatcga tcggctgacg ctgacacagg cgcgcatcga tgcgatggcc
     5281 ggcggcgttg cgaccgttcg cgacatcacc gatcccatcg gggtcgtgac cgagagctgg
     5341 cagcgaccga acggcatgat catcgagcgc gtgcgcgtgc cgctcggcgt cgtcgcggtg
     5401 atcttcgaaa gccgccccaa cgttgccgcc gacgccggcg tgctgtgtct gaaatccggc
     5461 aacgcggtga tcctgcgcgg cggctcg
//
LOCUS       DASWGM010000793         7803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1047878,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000793 DASWGM010000000
VERSION     DASWGM010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047878"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGA15_22000"
     CDS             complement(<1..287)
                     /locus_tag="VGA15_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011044137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9590404.1"
                     /translation="MMGHVTGASRDQATLFPEVLDEVVGRHDPVRVIDAFVDLLDLAE
                     LGFSKVAAEEMGRPPYAPGDLLKLYIYGYLHRIRASRRLEAETHRNVQVMWL"
     gene            complement(409..1143)
                     /locus_tag="VGA15_22005"
     CDS             complement(409..1143)
                     /locus_tag="VGA15_22005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9590405.1"
                     /translation="MSSRDSDRPPVLAHQRFVEEKESFAGLSLEQRFRRIHDTNLWGA
                     LASSSGLGSEIDATAMLRAELPRLLERIAVASLLDAPCGDAGWINQANLGVRYVGVDI
                     VPALIDRLRARAAEGEVRGEYHLADITRDPLPRCDAVLCRDALVHLSFANIERAVANF
                     KASGAVWLIATTFPEWQSNGDCEDGDWRALNFEMKPFNWGPPVELLNEHCLEAGGGWR
                     DKSLGVWRLSNVFPANAGTHTSRTPV"
     gene            complement(1203..1721)
                     /locus_tag="VGA15_22010"
     CDS             complement(1203..1721)
                     /locus_tag="VGA15_22010"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9590406.1"
                     /translation="MPDITRRDIANISAGAAIAGSTSSSRIANAVEFLEQSVSGERNM
                     AIQRYFIQEPTSGAPFISVAPIISLAAAHENIVYLCGVTADPAHPGDIKGQTKQVLER
                     IDRLLSKAGTDKSKLLSAQVWLTDMAHFEEHNAAWNAWVDPKNPPVRACLLSPQLWQP
                     GLLVEIMVTATK"
     gene            1827..2744
                     /locus_tag="VGA15_22015"
     CDS             1827..2744
                     /locus_tag="VGA15_22015"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9590407.1"
                     /translation="MDMDQIRTFVTVARGRSFSGAATALHRSQPAISRRIELLEREFD
                     IPLFERLRGGVVLTDAGAAFLPYAEIVLAAAQDGAEALRAGRQGDTGKISLALVGTLA
                     NAALTNVLRQFARKYPKLRLDLRTATSREVEELVQRGEATLGLRYSTGRNSGVVSEVI
                     AEERMVVVANPDHPLANGRRHQPGKLASERWVAFPTRYARESFVQFLQRKLLAVGLED
                     VEIIPIDSLTAQKRLVEAGFGIALLAESGIQEELKSGTLKVVNVPALQASIPIALVYR
                     RNGYLSAAARSLMSMIETSWSRPTARARR"
     gene            complement(2722..3804)
                     /locus_tag="VGA15_22020"
     CDS             complement(2722..3804)
                     /locus_tag="VGA15_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9590408.1"
                     /translation="MRAAIFRNGEIVVDTLPEPKPTAGQVLVKTLACGICGSDLHARK
                     HAHHMVELARHLPGRKPMDLTRDVVFGHEFCCEILDYGPATSRKLNIGARVCSLPALL
                     TSDGPQGIGYSNDNVGGYAERMLLSEALLLEVPNGLAAEHAALTEPLAVGIHAVAKAN
                     VRGDEVPLVIGCGPVGLAVIAALKLKGLQPIVAADYSPARRALAEKLGADIVVDPARK
                     EPYATWAEHAAMSPEQKAARPPLQAWRPALKPALIFECVGIPGLLQQVFEGAPRDARI
                     VVVGVCMETDRSEPTLGIMKELNVQYVLGYTPDEFAYSLRLIAEGQVDAASLVTATVG
                     IDGVATAFADLANPERHTKIIVEPWR"
     gene            complement(3913..4476)
                     /locus_tag="VGA15_22025"
     CDS             complement(3913..4476)
                     /locus_tag="VGA15_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9590409.1"
                     /translation="MRLPIIPPAKLSSEQKPLYDDMREGIAKNFQGFINVRDDGALLG
                     PWNPWIHEPQFGKPVWELTKAMVTKPSLPPAVREVAILVTGAHFRSAYELYAHVIVAE
                     HRGLSDEKLATIVAGQRPTDLSRQEAVAYDFASALVGGGVLPELTYRAAVEQFGQHGA
                     AELSYLVGLYCLVSVTLNTFDVPVPES"
     gene            complement(4594..5529)
                     /locus_tag="VGA15_22030"
     CDS             complement(4594..5529)
                     /locus_tag="VGA15_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9590410.1"
                     /translation="MSEKKSAERTAPARADRPFRGIALILASTVFLGASDATAKYLSA
                     TLPSIEIAWIRFLVFGMIMFPAMLPGSPLYALPSGRRGLQLMRGAALLASSLFFITGL
                     GFLPIAEASATGFVSPLFVTALSIVFLGESVGVRRWLATAVGLIGVLIILRPGSSAFH
                     PAAFFPIVSALAWACTMIMTRMMSGKEPVITTMTYSSIAGVCILSALVPFVWVAPSWH
                     DILFGIFIGIASTAGQWIVVLAFRYADASVLAPFQYTQLLWVSILGFVIFGELPDLWT
                     VTGAVFIVASGLYTAHRERVRRSQLPMQAEPSPNA"
     gene            complement(5755..6558)
                     /locus_tag="VGA15_22035"
     CDS             complement(5755..6558)
                     /locus_tag="VGA15_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9590411.1"
                     /translation="MSVIGQGTWYIDRGDRRGAVAALRRGVDLGMTHIDTAEMYGDAE
                     LVIAEAMAGRRDEIFLVSKVVPSNASRRGTVTACERSLARLKTDRLDCYLLHWRGSYS
                     LDETVAAFDQLVDAGKIRSWGVSNFDTDDLDELLGVAGDGKIACNQVLYHLGERAIEH
                     AVIPWCEQHGVAVVAYSPFGHNDFPPARSKAGQALQVIAEAHGASPRQVALSFLTRRP
                     SVFAIPKASSQEHAADNAAAANLTLSENEMTVLDKAFPRGPKPRSLPML"
     gene            complement(6766..7395)
                     /locus_tag="VGA15_22040"
     CDS             complement(6766..7395)
                     /locus_tag="VGA15_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9590412.1"
                     /translation="MTYSLPPLNALRAFEAAARHLSFKLAAHELHVTPAAVGQQVKAL
                     EARLGVRLFERLHKQLILTPAGQIFLPQISAGFRRIADATAQLRPSGAVLLQLGLHGS
                     FDLRRLALAEFRATHAEIGLRVLQPAGLHELIEGKVDLLIGRGLGHHPGYRCDRVSEG
                     SGLGDWLIAPAGTADCPEISSFRDWLRRLPAENGLATHRRRALASVSQS"
     gene            7525..>7803
                     /locus_tag="VGA15_22045"
     CDS             7525..>7803
                     /locus_tag="VGA15_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9590413.1"
                     /translation="MPRRIDHLIICVRDLAQAALSWQTLGFTLTPVGVHPFGTSNRLA
                     MFANNFLELLAVTDAAAIPAAAPGRFSFAAHNQDFLAAAEGMSMLAWHS"
BASE COUNT         1520 a         2470 c         2404 g         1409 t
ORIGIN      
        1 agccacatca cctggacgtt gcgatgcgtc tcggcttcaa gccgacggct ggcgcggatc
       61 cggtgcaagt agccgtaaat gtaaagcttc aacaggtcgc caggggcgta gggcggccgt
      121 cccatttctt ctgccgcaac cttggaaaat cccagctccg ccaggtcaag caagtctacg
      181 aaggcatcaa tcacccgcac cggatcatgg cgcccaacca cctcgtccag aacctcggga
      241 aacaacgtcg cttgatcgcg cgacgctccg gtgacgtgtc ccatcatcgc cctcgaatca
      301 aaaatcccct ccgtgccaca ctaacacacc cacttttcac acagcctgga atgccgggac
      361 ccataacccc gggcgcggat tgtttgcaga aggcctcgac gccggtggct aaacgggagt
      421 cctcgacgta tgggtcccgg cgttcgccgg gaagacgttg gaaagccgcc agacgccgag
      481 gctcttgtcg cgccagccgc cgccggcctc aaggcaatgc tcgttcagga gttcaaccgg
      541 tggtccccaa ttgaacggtt tcatctcgaa attcagcgcg cgccagtcgc cgtcctcgca
      601 atcgccgttg ctctgccatt ccgggaacgt cgtcgcgatc agccacaccg cgccggacgc
      661 cttgaaattc gcaaccgcgc gttcgatatt cgcaaacgac agatgcacga gagcgtcgcg
      721 gcacagaaca gcgtcgcatc gcggcagcgg gtcgcgggtg atatcggcga gatgatattc
      781 gcccctgact tcgccctcgg cggcacgggc ccgcaggcga tcgatcagcg cgggcacgat
      841 gtcaacgccc acatagcgca cgcccaaatt tgcctgattg atccagccgg catcgccgca
      901 gggcgcatcg agcagcgaag cgacggctat tctttcgagc agccgcggta attccgcgcg
      961 cagcatggcg gtggcgtcga tttccgagcc cagcccggaa ctggatgcca gagcacccca
     1021 caggttggtg tcgtggatgc ggcggaagcg ttgctccagg ctcaagcccg caaaggattc
     1081 cttttcctca acaaagcgct gatgcgcgag aacgggcgga cgatcggaat cgcgagagga
     1141 catgacgcac aatatcacac cggcgcgccg ccccgatgaa ccggcgcacg ctcgacggca
     1201 gcttatttgg tggcggtgac catgatctcg accagcaacc ctggttgcca caattgaggt
     1261 gaaagcaggc atgcgcgcac gggagggttc ttgggatcca cccatgcgtt ccaggccgca
     1321 ttgtgttcct cgaaatgcgc catatccgtc aaccatactt gcgcgctcaa gagcttcgat
     1381 ttgtcggtgc ctgccttgct cagcagccga tcgatccgct cgagaacctg cttggtctgg
     1441 cccttaatgt ctccggggtg tgcgggatcg gcagtgacgc cgcatagata gacaatattc
     1501 tcgtgagcgg cagccaagct gatgatgggt gcgacgctga tgaatggagc gccgctggtc
     1561 ggttcttgaa tgaaatagcg ctgaatggcc atattgcgtt ctcctgaaac actctgttcg
     1621 aggaactcga ccgcattcgc gattcgagac gacgaggtac ttccggcaat ggccgcgccc
     1681 gcggaaatat tggcgatgtc tcgccgtgtg atgtcgggca tgtcaagaac tcccgccgcc
     1741 tttcagttcg ggcaaaatca actggtattg cacatttgtc caatgcatag tttttagcat
     1801 tagcatcagc tatttggatg tcattgatgg acatggatca gatccggact ttcgtgacgg
     1861 ttgcccgcgg ccgcagcttc agtggtgcgg cgacggcgtt gcaccgctcg cagccggcga
     1921 tcagccggcg catcgaattg ctggagcgcg aattcgacat cccgttattc gagcggctcc
     1981 gcggcggcgt tgtgctcacc gatgccggcg ctgcatttct tccctatgcg gagatcgtgc
     2041 ttgccgcggc acaggacggc gccgaagcgc tgcgcgccgg acgccagggc gataccggca
     2101 agatttcgct ggcgctggtc ggcacgctcg ccaacgccgc gctgacaaat gtcctgcggc
     2161 agttcgcacg aaaatacccc aagctgcgcc tcgatctgcg gaccgcgacc agtcgcgaag
     2221 tcgaagagtt ggtgcaacgg ggcgaggcca cgctgggttt gcgctattcg accggccgca
     2281 attccggcgt cgtgtcggag gtgattgccg aagaaagaat ggttgtcgtc gccaatccgg
     2341 atcatccgct ggccaatggc cgcagacatc agccgggcaa gcttgccagc gaacgctggg
     2401 tggcatttcc gacgcgctat gcgcgggaga gtttcgtgca gtttctccag cgcaaactgc
     2461 ttgccgtcgg cctggaggac gttgaaatca ttccgatcga cagtttgacc gcccagaagc
     2521 ggctggtcga agccgggttc ggaattgcgc tgctggcgga gagcggcatt caggaagagc
     2581 tcaaatccgg caccttaaag gtggtgaacg tccccgccct gcaggcgagc ataccgatcg
     2641 cgcttgtcta ccggcgcaac ggctatttga gcgccgccgc gcgcagcctg atgtcgatga
     2701 tcgagacctc gtggagccga cctaccgcca gggctcgacg atgatcttgg tatggcgttc
     2761 cggattggcc aagtcagcga acgcggtggc gacgccgtcg atgccgacgg ttgcagtcac
     2821 cagcgaggcc gcgtcgacct gcccttccgc gatcaggcgc agcgaatagg caaactcgtc
     2881 gggcgtatag cccagcacat attgcacatt gagctccttc atgatgccga gcgtcggctc
     2941 ggagcggtcg gtttccatgc agacgccgac cacgacaatg cgggcatcgc gcggcgcgcc
     3001 ttcgaagacc tgctgcagca ggcctggtat cccgacgcat tcgaagatca gcgccggttt
     3061 cagggccggc cgccacgcct gcagcggcgg ccgcgcggcc ttctgctccg gcgacatcgc
     3121 ggcgtgctcg gcccaggtcg cataaggttc cttgcgcgcg ggatcgacca cgatgtcggc
     3181 acccagtttc tcggccaagg cgcgccgcgc cggcgaataa tccgccgcca cgatcggctg
     3241 caagcctttc agtttcagcg ccgcgatcac ggccaatccc accgggccgc agccgatcac
     3301 cagcggcacc tcgtcgccgc gcacattggc tttggcgacg gcgtggatgc cgaccgccaa
     3361 cggctcggtc aatgccgcat gttcggccgc aagtccgttc ggcacctcca gcagcagcgc
     3421 ctcgctgagc agcatgcgct cggcatagcc gccgacattg tcattggaat agccgatgcc
     3481 ctgcgggcca tccgatgtca gcaacgccgg gagcgaacac acccgggcgc cgatgttcag
     3541 cttgcgcgat gtggccgggc cgtagtcgag aatctcgcag cagaattcat ggccgaacac
     3601 gacgtcgcgg gtgaggtcca tcggcttgcg gccggggaga tgtcttgcca gttcgaccat
     3661 gtggtgggcg tgcttgcgcg catgcaggtc cgagccgcaa atgccgcagg ccagcgtctt
     3721 gaccagcacc tgccccgcgg tgggtttagg ctccggcagc gtgtcgacga cgatctcgcc
     3781 gttcctgaaa atggcagcgc gcatcggcga tcccctcacg gtttgtgatt gtccgccctc
     3841 ataccacgga ggctcagaac atgtaacgtc tacccggcaa ggcttgctgc tgaccgcctg
     3901 cgtcgtatgc tctcaggact ccggcaccgg gacgtcaaac gtattcaagg tcacggacac
     3961 cagacaatac aatccgacga gataggacag ttccgccgcc ccgtgctggc cgaactgctc
     4021 gaccgccgcg cgatacgtca gttccggcag aacgcccccg ccgaccagcg cggaagcaaa
     4081 atcgtaggcg actgcttctt gccgcgacag gtccgtcggg cgctggccgg caacgatcgt
     4141 cgccagcttc tcgtcggaca gcccccggtg ctccgcgacg atgacgtggg catagagttc
     4201 ataggcggag cggaaatgcg cgccggtgac cagaatggcg acttcacgta cggccggcgg
     4261 cagcgacggc ttcgtcacca tcgcctttgt cagctcccac accggcttgc cgaattgcgg
     4321 ttcgtgaatc cagggattcc acggcccgag cagcgcgccg tcgtcccgga cattgatgaa
     4381 tccttgaaaa ttcttggcga tgccctcccg catatcgtca taaagcggtt tctgttcgga
     4441 actgagcttc gcgggtggaa tgattggcag gcgcatgtcg caatctccat ctggcccccg
     4501 gcctaaggat gatcgtcgct cgacgaggcg atggcaaacg aattggactt tcggcggcat
     4561 cgtctcacgt cgacgtgaaa gaacgtcggt ggatcacgcg ttgggcgacg gctcagcctg
     4621 catgggaagc tgcgagcgcc tgacacgctc tcggtgtgcg gtgtagagac cgctggcgac
     4681 gatgaacacc gcgccggtca cggtccagag gtccggcaat tcaccgaaga tcacgaagcc
     4741 gaggatgctg acccacaata gctgggtgta ctgaaatggc gcgagcaccg aggcatcggc
     4801 ataacgaaac gcgagcacga cgatccattg gcccgccgtc gatgcaatac cgatgaagat
     4861 gccgaaaagg atatcgtgcc agcttggcgc gacccaaacg aacggcacca gtgccgacaa
     4921 aatgcagacg cccgcgatcg acgaataggt catggtggtg atgacgggtt ccttgccgct
     4981 catcattcgc gtcatgatca tggtgcaggc ccaggccagc gccgagacga tcggaaaaaa
     5041 cgctgcggga tgaaacgcgc ttgagcccgg ccgcaggata atcagcacgc cgatcaatcc
     5101 gaccgcggtc gcgagccagc ggcgcacgcc gacgctctcg ccgagaaaaa caatcgacag
     5161 cgcggtgacg aacagcggcg agacaaatcc ggtagcggat gcttccgcga tcgggagaaa
     5221 accgaggccg gtgatgaaaa acagcgaaga tgccagcagc gcggcgccgc gcatcaactg
     5281 caggccgcgg cgcccggatg gcaaggcata caacggcgaa cccggcagca tcgccggaaa
     5341 catgatcatc ccgaaaacca ggaatctgat ccaggcgatc tcgatcgacg gcaacgtcgc
     5401 cgagagatat ttcgcggtgg cgtccgaggc cccgagaaat acggtcgacg caaggatcag
     5461 cgcgatgccg cgaaacggcc ggtcggcgcg cgcgggtgcg gttcgttcgg ctgatttttt
     5521 ctcggacaac ggtaatgagt cgctgtccag cctggcggct gcggcggggg gcggcgtcac
     5581 aattgaaact caggcgtaat gatcgaatcg ggcgagagct caaaaaacga taattgcctg
     5641 tcatcgacaa ggtccgaaaa caggcagccg atatgcgcga tcgtgtgcgg cgactggtca
     5701 cgtcaccgct gaaacctttt gtgaggagcc gtgcgttgtg aggagtcgtg cgcgttacag
     5761 catcggcagg ctacgcggct ttggaccgcg cggaaatgcc ttgtccagta cggtcatctc
     5821 gttttcgctg agcgtgagat ttgccgctgc cgcgttgtcg gccgcgtgtt cctgactcga
     5881 ggcttttgga atcgcgaata cggaaggtcg gcgcgtcaga aagctcaagg cgacctggcg
     5941 cggcgatgcg ccgtgggctt cggcgataac ctgcaacgcc tgccccgcct tgctgcgcgc
     6001 cggaggaaaa tcgttgtgac cgaacggcga ataggcgacc accgcgacgc cgtgctgctc
     6061 gcaccacgga atcaccgcat gctcgatcgc gcgttccccg agatgataga gtacctgatt
     6121 gcaggcgatc ttgccgtcgc cggcaacccc caacagttca tccagatcgt cggtatcgaa
     6181 attgctgacg ccccagctgc ggattttccc tgcatcgacc agttgatcga acgcggccac
     6241 cgtctcatcc agcgaatagg atccgcgcca atgcagcaga tagcaatcga gccggtcggt
     6301 tttcaaccgc gccagcgaac gctcgcacgc cgtgacggtg ccgcgccgcg atgcgttgct
     6361 cggcacaact ttcgaaacca gaaaaatctc gtcgcgccgc ccggccatcg cttccgcgat
     6421 cacaagctcg gcgtcgccat acatctcggc ggtgtcgatg tgggtcatgc cgagatcgac
     6481 gccgcggcgc aacgcggcga cggcgcctct gcgatccccg cggtcgatgt accaggtgcc
     6541 ctgcccgatc acggacacct cgcgtttgct gttgccgaac ggccgagatt tcgtttcgat
     6601 cgatccatgg tccgatttgc caatgttatt tcaactcgct gatatccgcg accaggacgg
     6661 tgctcgtcac tgattccgtc acatagaggc ggtcgcggtt cttgccgccg attgcgacat
     6721 ttgtacagct cgatccgacg caggacttgc cggcatcgtg ctcgatcaac tttggctgac
     6781 gcttgccaac gcacggcggc gatgcgttgc gagcccgttc tcggccggca gccggcgcag
     6841 ccaatcgcgg aagcttgaga tctcgggaca atccgccgtg ccggcgggcg caatcagcca
     6901 gtcgccgagc ccggatccct cgctcacgcg atcgcaacga tagcccgggt ggtggccgag
     6961 accgcggccg atgagcaagt cgaccttgcc ctcgatgagt tcgtgcaggc ccgcgggctg
     7021 cagcacccgc aagccgattt cggcatgcgt tgcacggaac tcggcaagcg ccaggcggcg
     7081 gaggtcaaag cttccgtgca gacccaactg cagcagcacc gcgcccgacg gcctcaattg
     7141 cgcggtcgca tcggcgatac gacgaaagcc ggcggatatt tggggcagga agatttggcc
     7201 ggccggtgtc agaatgagct gcttgtgcag ccgctcgaac agacgcacgc cgagccgcgc
     7261 ctccaaggct ttcacctgct gtccgacggc ggcaggtgtc acgtgaagct cgtgcgccgc
     7321 cagcttgaaa ctgagatgtc gcgccgcggc ttcgaacgcg cggagcgcat taagcggtgg
     7381 gaggctgtag gtcatcgccg cccagtttgt caccgatacg cgctggcctt gcaatagatt
     7441 ttcttgagct cggcgtcagg aacaatgctt tgccctgcgt tcaccgcaca agctaccgtc
     7501 cggttcacaa gcccggagac tcccatgccc cgccgcatcg accacctcat catttgcgtc
     7561 cgcgatcttg cgcaggccgc actgagttgg caaaccctcg gttttaccct cacgccggtc
     7621 ggcgttcatc cgttcggaac cagcaaccgc ctggcaatgt ttgcgaacaa ttttctcgag
     7681 ctgctggccg tcaccgacgc cgcggcaata ccggccgccg cgcccggccg attcagcttc
     7741 gctgctcaca accaggattt tctcgccgcg gcggagggga tgtcgatgct ggcctggcac
     7801 agc
//
LOCUS       DASWGM010000794         4522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1455595,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000794 DASWGM010000000
VERSION     DASWGM010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455595"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VGA15_22050"
     CDS             complement(<1..497)
                     /locus_tag="VGA15_22050"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9590414.1"
                     /translation="MHLPVPLRGMLSVPFRAFGIRPSKMNPNTCTICELMFTAVMKAR
                     KVSVDATVLFADLRGYTGLSQSLSPDAISGLLDTFYDECANAIWEYDGLLNKTIGDAV
                     MAIFNFPIRGEDHAIRAVRAARSIQKRWASERENLAKTLGVDGSELGVGIGIHSGELS
                     FGEFGR"
     gene            complement(896..4285)
                     /locus_tag="VGA15_22055"
     CDS             complement(896..4285)
                     /locus_tag="VGA15_22055"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9590415.1"
                     /translation="MRIMAGTSWVSVRSAVIGGHALGAHGRQRSPCGVPCSGSSSQTT
                     ESGAVRCIKCELENRAGRKFCASCGTALPVPCESCGFPNEAAERYCGGCGHALVGEKI
                     AAVEAGRNVDPGGDRRPVTVLFCDLVGYTRLSSVLDAEDVHAMLERFFALVDATVDRF
                     GGTIDKHIGDAAMALFGAPLARGNDAERAVRAALEIQASVPKLASGLPSALAVHIGMA
                     TGEVIASSVGSQHHRGYTVTGEAANIAARLLEKAVSGETLVSDDVYQATNHVVSYQPL
                     GPLALKGVRHSVEAWRPTGIRSSAPETHALVGRRSELGQFRAVLDACVNGATGTAVLI
                     RGEAGIGKTRLMDELQSIAAASGMSCTAGFVLDFGTTRGHGAVRTVVAGLLGLGSEVT
                     PDIAECAIDEILKDGRLQADDALYLRDLLEMSPPETMRKHYEAIDAAARTHGKERVVT
                     ALAKASADSRPLLVTVEDVHWADPETLSLLAAITRATAMSRTVLVMTTRIEGDPLDAP
                     WRSLAGGGTLITVDLSPLSAADARSIARRFIDVAAFADQCVERAGGNPLFLEQLLRGA
                     RDLTDGRLPASIQSVVLARTDLLSAQDRRAIQAASVLGQRFNLAHLRALLQEPQYDCD
                     TLVRNVLIRPTRDGLQFAHALVRDGVYGSLTNARKRELHGAAAVIFIDDPVLRAEHLD
                     RAGDPQAARAYLAAANAQAILFRQDQAIALAARGLALATKAHDTFELAMLIGDLQLDA
                     GRGTEALEGYVQALAVASEDADRCRALLGCASSNRLIARVEDAFSGLAEAEPLAVVHI
                     DDPALAEIHYLRGNLHFARGHLAECRSEHELALAAARRVESPEWQARALSGLADAQYM
                     DCRMATALSHFAGCVDLCEAHDLTWIAVPNRVMMGHCRIYTCEFDLGSEDMRVALEAA
                     VRIGNRHAEMFATNSIGMCLTAAGRYVEADNVQAKALAQSRAIKARRYEAVILGHSAE
                     VALSRGLRAEALTLAREGREISEETGPGFVGPILYGLLALVEDKRTDQEVALAAGEAL
                     LNQGCVGHNYLWFRRYAIERALLLEDWNEVDRHADALLLRMANEPLAYASHVAARGHY
                     LARRGLGEATEMGEDKHRLLRATAAEAGWRIDALGSALRQM"
BASE COUNT          878 a         1479 c         1378 g          787 t
ORIGIN      
        1 cggccgaatt cgccaaagct cagttcaccg gaatgaatac caatgcctac ccctaattcg
       61 ctgccatcga cgccaagcgt cttggcgagg ttttcgcgct cggacgccca acgtttctgg
      121 atcgagcgcg cggcgcgtac agcacgaatg gcatgatctt cccccctgat agggaaattg
      181 aagatagcca tgacagcgtc tccaatggtc ttgttgagca acccgtcata ctcccaaatg
      241 gcgttcgcgc attcatcgta gaacgtgtcg agcagccctg atatcgcgtc cggcgacagg
      301 gactgcgaca ggccggtgta gcctctcaga tcggcgaaca gcacggtggc atcgacagag
      361 acctttcgag ccttcatcac cgccgtgaac atcaactcgc agattgtgca ggtattcggg
      421 ttcatcttgc tcggacgaat gccgaacgct cgaaatggca ccgagagcat cccgcgcagt
      481 ggcacaggca gatgcatctg ctgccaacac cccttgcaga tcctcgaccg ttgcggagtc
      541 atcaggcgcc gaccttagcc gtcatatgcg gaatgaccta ctctagccta tcatcgggag
      601 caagcactca ccacccggca ccggtctcgt ttattgagtc tggctgccaa gccagtcgat
      661 cgcccaggtg ccagacaggt cgatccgccg tgctcgcgcc gttcgcccct ggcgcacaat
      721 cccgatcgac cgcgtcaaca tctgctgccg cggcgagctt ccgcaggcag cgcgcccgct
      781 gcattcatgg cactgctgaa gggcgccaat ttcggcaagc agctagtgcg cgtcgggccg
      841 gacaaggcgt aagccgacgt ccacagaggc ccgccatcgc cggcgcggcg cagagttaca
      901 tctgacgcaa tgcgctgccg agtgcgtcga tgcgccagcc ggcctcggca gcggtcgcgc
      961 ggagaagcct atgcttatct tcgcccatct cggtggcttc gcccagtccg cgacgggcca
     1021 ggtaatggcc gcgcgcggcc acgtgggacg cgtacgcgag cggctcgttg gccatgcgaa
     1081 gaagcagcgc atcagcgtgc ctatcgactt cattccagtc ctcgagcagc agtgctcgct
     1141 cgatagcata tcgacggaac cagaggtagt tgtggccgac gcatccttga ttcagtaacg
     1201 cttctccggc ggcgagagcc acctcttgat ccgtccgctt gtcctccacc agagcgagga
     1261 gaccgtagag tatcggacca acaaacccgg gtccggtctc ctctgaaatc tctcgaccct
     1321 cgcgggcaag tgtcaacgct tctgctctca acccccttga tagagcgacc tccgcgctat
     1381 ggccaaggat cacggcttcg tagcgtcgcg ctttgatcgc ccgcgactgc gccagcgcct
     1441 tggcttgcac attatcagcc tcgacatacc gtccggcagc ggtcaggcac atacctatcg
     1501 aattggtcgc aaacatctcc gcatgcctgt taccgatcct cacggcggct tcgagcgcga
     1561 cgcgcatgtc ctccgatccg aggtcaaact cgcaggtgta aatccggcaa tgccccatca
     1621 tgacgcggtt aggcactgca atccacgtca ggtcatgcgc ttcgcaaaga tcgacacaac
     1681 cggcaaagtg ggaaagcgcg gttgccatgc ggcaatccat atactgcgca tccgccaacc
     1741 cgctcagcgc tcgggcttgc cattccggag attccacgcg tcgcgcggcc gcaagagcca
     1801 attcatgttc gctgcggcac tcggccaggt gcccccgcgc aaagtgcaga ttgccgcgga
     1861 gatagtggat ttcggcgaga gcgggatcgt cgatgtgcac gactgccaag ggctcagctt
     1921 cggccaggcc ggaaaacgcg tcctccaccc gggcgatcaa acgattggac gaggcgcaac
     1981 cgagcaaggc gcggcaccgg tcggcgtcct cgctcgccac tgccaaggcc tggacgtagc
     2041 cttcgagggc ctcggtgccg cgccccgcgt ccaactgaag atcgcctatc agcatggcaa
     2101 gttcgaatgt gtcgtgtgcc ttggtggcga gcgccaggcc gcgcgccgca agcgctatcg
     2161 cctggtcctg gcggaacagg attgcctgcg cattggccgc cgcaagatag gcgcgcgctg
     2221 cctggggatc gcccgcacgg tccagatgct cggctcgcag caccggatca tcgataaaga
     2281 tcacggccgc ggcaccgtgc aactctcgct tccgcgcgtt ggtgagcgat ccgtagacgc
     2341 cgtcgcgtac caacgcatga gcgaattgca gaccgtcccg cgttggacgt atcaggacat
     2401 tccgtaccaa cgtgtcacaa tcgtactgcg gctcctgcag tagcgccctg aggtgggcga
     2461 ggttgaagcg ctggccgagc acggaggctg cctggatggc gcggcggtcc tgggcagata
     2521 gcagatccgt acgggccaac acgacgctct gaatggatgc cggcagccgt ccgtccgtga
     2581 gatcgcgggc gcctcttagc aattgctcca ggaacagcgg attgccgccg gcacgctcga
     2641 cgcactgatc ggcgaaggca gcgacgtcga tgaagcgtcg cgcgatcgac cgcgcgtccg
     2701 cagccgacag cggagaaagg tctacggtga tcagcgttcc accgccggcg aggctgcgcc
     2761 aaggcgcatc gagcggatcg ccttcgatgc gagtggtcat aacgagcacc gtacggctca
     2821 tcgctgtagc gcgcgtgatg gcggcaagaa ggctcagcgt ttccggatct gcccaatgca
     2881 cgtcctcgac ggtgacgaga agcggccgac tgtctgccga agccttggcc agagcggtga
     2941 ccacgcgctc cttgccgtgg gtccgtgcag ctgcgtcgat cgcctcatag tgcttgcgca
     3001 tggtttcggg cggagacatc tcgagaaggt cacgcagata gagcgcgtca tctgcctgca
     3061 gacgtccgtc tttcaaaatt tcatcgatcg cgcactcggc aatgtctggc gtgacctcgg
     3121 agccaagccc caaaagcccg gcaaccaccg tgcgcacggc gccgtgaccg cgcgtggtac
     3181 cgaaatcgag gacgaatccg gcggtgcacg acatcccgga cgccgcggcg atcgactgca
     3241 actcgtccat cagacgggtc ttgccgatcc cggcctcacc gcggatcagc acggcagtac
     3301 cggtagcgcc gttgacgcag gcgtccagca cggcgcggaa ctgcccgagc tcggatcgac
     3361 ggccgactaa tgcatgggtc tccggggccg agcttctgat tcccgtcggt cgccaggcct
     3421 cgacggaatg ccggacgccc ttcagtgcca gcggtcccaa cggctggtag gaaacgacgt
     3481 gattggttgc ctgatagaca tcgtcggaaa caagcgtttc gccgctgacc gccttctcca
     3541 aaagccgcgc ggcaatgttg gcggcttcac ccgtgacggt atagccgcga tggtgctggc
     3601 ttcccaccga actcgcgatg acttcaccgg tcgccatacc gatatgaacg gccagggcgg
     3661 aaggcagacc ggacgcgagc ttcggaacgg atgcctggat ctccagggcg gcccgcacgg
     3721 cgcgctcggc atcgttgccg cgcgcgagcg gcgctccgaa cagcgccatt gccgcgtcgc
     3781 cgatgtgctt gtcgatcgtc ccgccaaagc ggtcgacggt cgcatcgacg agcgcaaaga
     3841 agcgttccag catggcatgg acatcttcgg catccagcac cgaagagagg cgggtatagc
     3901 cgaccaagtc gcagaacaac acggtaaccg gacgccgatc tccccctgga tcgacattcc
     3961 gtccggcttc gaccgccgca atcttttcgc cgacaagggc gtgaccgcag ccgccgcaat
     4021 aacgttcggc cgcctcgttc ggaaagccac agctctcgca gggaaccggc aatgcggtgc
     4081 cgcaagacgc acagaatttg cgtcccgccc ggttctccaa ttcgcacttt atgcaccgca
     4141 cagcgccgga ctccgttgtc tgggatgatg agccggagca agggacaccg caaggagatc
     4201 gttgccggcc gtgcgctccc aacgcatgtc caccgatgac ggctgaacgg acggataccc
     4261 agctggtgcc ggccatgatc cgcatcggct agcgtgcagg cggaaaacag cggaagcggt
     4321 gcgcgcgcaa tcgacgattt cgggctagcg cacaatcccg atcgagcgcg taaatatctg
     4381 ctgccgcggc gaggttccgc aggcaaacgc aaaggtcatg gtttggccgg cggccacgac
     4441 gtcgatctcg gcaccaaaat atttcatgtc ctggttgccg agatagtcgg ccggcgccgt
     4501 cgtgatgcga ctggctttga tc
//
LOCUS       DASWGM010000795         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1314625,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000795 DASWGM010000000
VERSION     DASWGM010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314625"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /gene="rpsL"
                     /locus_tag="VGA15_22060"
     CDS             complement(<1..318)
                     /gene="rpsL"
                     /locus_tag="VGA15_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX9590416.1"
                     /translation="MPTINQLIASPRVVQKSRKKVPALQQSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIPGEGHNLQEHSVVMIRGGRVKDLPGVRYHILRGVL
                     DTQG"
     gene            complement(1225..>2873)
                     /locus_tag="VGA15_22065"
     CDS             complement(1225..>2873)
                     /locus_tag="VGA15_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590417.1"
                     /translation="LGEEDFEALVKILSVLAAAGIVTSVVAVWYERWDAELAATLISD
                     VRLRLFEHVQNLPSAYFARTKRGEILSRFSIDLSAFEGSIKSFANSAALPFFELIAGI
                     VLMLFLNWQLAAVALLVFPITLIGPRILTPKAVQANYEQKINESALLAMVQENVAAQA
                     VIKAFSLQRRTLGWFTMRNDDARRKIAAAAFLSTMVERTVTISVLLLHLVVLALGAYL
                     ATKGQITIGTFVTFESAFWEVSYNIAHLMHFIPVSIQSAAAVRHIQELLDEPTRGADR
                     PGAPDLPRITNDITFDRVTFQYEGSQTPVLDNLSLKLKVGKSIAIVGPSGSGKSTLLN
                     LILRLYVPDEGRVTIDGVDIRKVTRESLRRSMAVVFQENMLFNMSIRENIRLGKEGAT
                     DEEVEQAARKAEIHRYIMSLPQKYDTSVGERGDTLSGGQRQRIAIARAIVRNPSILLL
                     DEATSALDQTTEAAINRTLLKVAKGRTMIFSTHRLTSVVDMDEIIVISGGKAIERGSH
                     AKLLAANGVYRKLWDDQGYTPHDAADQADDDSDDDDEEDR"
BASE COUNT          543 a          874 c          879 g          577 t
ORIGIN      
        1 gccctgggta tcgaggacgc cgcggagaat gtggtaacgc acgccgggca aatccttgac
       61 gcggccgccg cggatcatga ccaccgagtg ttcctgaagg ttgtggcctt cacccggaat
      121 gtagccgatg acctcgaagc cgttggtcag gcgcaccttt gcgaccttgc gaagcgccga
      181 gttcggcttc ttcggggtcg tggtgtagac gcgcgtgcaa acaccgcgct tctgcggcga
      241 ctgctgcagc gccggcacct tcttgcgcga cttctgtacg acgcgcggac ttgcgatcag
      301 ctggttgatc gtcggcatgt cagccttcac cctttaattc gtgcgaaacc ggaaaacggc
      361 tgcgcaaatt ctcaatcggg actggaagtc ccatccggtc gcctcgcgaa ttgaacccgc
      421 gcaaagcgaa atcgcgccaa ctgctcattg ctgagcggaa agcgcttcga cgccacagag
      481 gaccgcaatc gtaactggtc agcgtatgcc gttcagtcag gtaccgaggt catgcatccg
      541 aaatcaatct caagaggact tgctcaagag aaccaaaatc gtcctggcat ttgcctagct
      601 atattcgaca gcgtttgagc ggcattcgtc gaggttggtg cccgtctcga tccccggaga
      661 aagttgccca agggaattga agggtgatcc gttccgactg tggcgtcgct caccgcctgt
      721 cgttaagtgc gcgggtttta tcggcggtgg atagctaagt caagacgaaa ggacaatgta
      781 agaagcgcct ctggcgcctg cggaatcggc gtgcgcttga atttcggagc aggtgaacca
      841 acgctcttgg cgcgggtagg ccaacgattc gtacgaacct gtccaccgcc ctcttccgcg
      901 tgctcttgat gtagccggcg caggatgagc ctcaccgcaa tattatccgg gtaatatagc
      961 ctttttgggc cggttgaggg gatgccgtgc ccattttctc ggctggcggc tctgaaagcc
     1021 ggttacggaa cccctggcgc cggctccgaa gacgccgagc tgccggccag cttccaccct
     1081 gtagcctagc gatttgcccg gcccgacatc gaccagcacc tgggcagcaa tgcgtcgcca
     1141 ttccgagccg gcttcgaaat cgctggattt ggcgctaatg gtctctgccg gggtggcgaa
     1201 ttcgccggtt cgaagacgcg gccttcaccg gtcctcctca tcatcgtcgt cgctgtcgtc
     1261 gtcagcctga tcggccgcgt catgcggcgt gtagccctga tcgtcccaca gctttcgata
     1321 cacgccattg gcggcgagca gcttggcatg cgagccgcgc tcgatcgcct tgccgcccga
     1381 tatcacgatg atctcatcca tatcgaccac cgatgtcagg cggtgggtcg agaagatcat
     1441 ggtgcggccc ttggcgacct tcagaagcgt gcgattgatc gcagcttcgg tggtctggtc
     1501 gagcgccgag gtcgcctcat cgagcagcag gatcgacgga ttgcggacga tcgcccgggc
     1561 gatcgcgatg cgctggcgct gcccgcccga cagcgtatcg ccgcgttcgc cgaccgaggt
     1621 gtcgtatttc tgcggaagac tcatgatgta gcgatggatt tcggccttgc gcgccgcctg
     1681 ctcgacctct tcgtcggtag caccctcctt gccgagccga atgttttccc ggatcgacat
     1741 gttgaacagc atgttttcct ggaacaccac cgccatgctc ctgcgcagcg attcgcgggt
     1801 cacctttcgg atatcgacgc cgtcgatggt cacgcgccct tcatccggca catagagccg
     1861 gaggatcaga ttgagcagcg tgcttttgcc ggagccgctg gggccgacga tggcaatgct
     1921 cttgccgacc ttgagcttga gactgagatt gtcgagaacc ggcgtctggc tgccctcata
     1981 ctggaatgtc acgcggtcga aggtgatgtc attggtgatg cgcggcagat cgggggcacc
     2041 cgggcggtcg gcgcctcggg tcggctcgtc cagcagttcc tgaatgtggc gcaccgccgc
     2101 tgccgactgg atcgacaccg ggatgaaatg catcaaatgg gcgatgttgt aggaaacttc
     2161 ccagaacgca ctttcgaacg tcacgaaagt cccgatggtg atttggccct tggtggcgag
     2221 gtaggcgccg agcgccagca ccacgaggtg cagcaacaac acggagatcg tgaccgtgcg
     2281 ctcgaccatg gtcgacagga atgccgccgc cgcaattttt cggcgcgcgt cgtcgttgcg
     2341 catcgtgaac cagccgagcg tccggcgctg caggctgaag gccttgatga ccgcctgcgc
     2401 cgcgacgttt tcctgcacca tggcgagcag tgccgattca ttgatcttct gttcgtaatt
     2461 cgcctgcacc gccttcggcg tcagaatccg cgggccgatc agcgtgatcg ggaacaccag
     2521 cagcgccacc gcggccagct gccagttcag gaacagcatc aggacgatgc cggcgatcaa
     2581 ctcaaagaaa ggcagcgccg cgctgttggc aaaactcttg atcgaccctt cgaacgccga
     2641 cagatcgatc gagaagcgcg agagaatctc gccgcgcttg gtgcgcgcaa aataagccga
     2701 cggcaggttc tggacgtgct cgaacaggcg caaccggacg tctgaaatca gggttgcggc
     2761 gagttcggcg tcccatcgtt cgtaccagac cgcaacgacc gaggtgacga tacccgcggc
     2821 cgccagtacc gagagtatct tgaccagcgc ctcgaaatct tcctcgccga gcg
//
LOCUS       DASWGM010000796         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1592801,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000796 DASWGM010000000
VERSION     DASWGM010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592801"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VGA15_22070"
     CDS             complement(<1..884)
                     /locus_tag="VGA15_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590418.1"
                     /translation="MASPSLIPAGDYRTTYAADTTIFPTATSRNAAIAGIVLICFAPL
                     VFSEYWLSILIQIGIFAIAALGLNILVGFTGQISIGHAAFFLFGAFTSAYISNTLPIP
                     VFFAIPLAGVVTALVGLIFGVPAARLKGLYLVIATLAAQYILLDFFSRADWFSGGSVP
                     ASANPFSIFGYTFRGDRQYFYVVLAYVLVSYLLVTNLMRTRDGRALVAIRDHYLSAEI
                     MGINLTKYRTLSFGLAAFFAGIAGALYAHYQLVVSNEGFGIERSILFLAMVIIGGTGS
                     IMGTLMGTAFVVLLPESM"
     gene            complement(890..1783)
                     /locus_tag="VGA15_22075"
     CDS             complement(890..1783)
                     /locus_tag="VGA15_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590419.1"
                     /translation="MNIQFLIQLLVNGLVVGTLYGVVAMSFVLIYKATQVVNFAQGEL
                     LLVGAWVCWALLTKYQVPFWVGMPLTLVFMFVFGIAIQIVILRPMIGEPIISVIMVTI
                     GLSTVLQASLKWIFGVNPQPFPRVFTSQSVNFLGLQIQTVYVMSLVVSVAMMVGMAWF
                     FRVSKYGLAMRATAFNQQVAQSLGISVKNVFAMAWAISATVSAVAGVVVAVVNGVSSG
                     LSAYGIKVFPAAILGGLDSIGGAVLGGIIIGLLENIAQYVDSEYLHWGNLYEIAPFYV
                     LIIILMIKPYGLFGTKDIERV"
BASE COUNT          498 a          615 c          623 g          346 t
ORIGIN      
        1 tccatcgatt ccggcagcag caccacgaag gcggtgccca tcagcgtgcc catgatcgat
       61 ccagtgccac cgatgatcac catcgccagg aacaggatcg agcgttcgat cccaaaacct
      121 tcattcgaca ccaccagctg ataatgcgca tagagcgcgc ccgctatccc tgcaaagaac
      181 gccgcgagcc caaatgacag cgtgcggtat ttggtgagat tgatgcccat gatttccgcg
      241 gacagatagt ggtcgcggat cgcgaccagc gcgcggccgt cgcgggtgcg catcaaattg
      301 gtcaccaaga ggtagctgac caggacatag gccagcacca cgtagaaata ttgccggtcg
      361 ccgcggaagg tgtagccgaa gatcgagaac gggttggcgc tggccggcac cgagccgccg
      421 gagaaccagt cggcgcgcga gaagaagtcg agcaggatgt attgcgcggc cagcgtcgcg
      481 atgacgagat aaagcccctt cagccgcgcc gccggcacgc cgaagatcag gccgaccaac
      541 gcggtgacca cgcccgccag cggaatggca aaaaacaccg ggatcggcag cgtgttggag
      601 atataggccg aggtgaatgc cccgaacaaa aagaacgcgg catggccgat cgaaatctgc
      661 ccggtaaagc cgaccagaat gttcaggcca agggctgcga tcgcaaaaat accgatctgg
      721 atcaggatgc tcagccagta ttcgctgaac acgagcggcg cgaaacaaat cagcacgatg
      781 ccagcgatcg ccgcattgcg gctggtcgcg gtcggaaaga tcgtggtgtc ggccgcgtag
      841 gtggtgcggt agtcgccggc cggaatcagg gaagggcttg ccatatgggt tatactcgct
      901 cgatgtcttt ggtgccgaac aagccgtagg gcttgatcat caggatgatg atcagcacgt
      961 agaacggcgc gatttcatat aaattgcccc agtgcagata ttcgctgtcg acatattgcg
     1021 cgatgttttc caacaagccg atgatgatgc cgccgagcac cgcgccgccg attgagtcca
     1081 acccgccgag gatcgccgcc ggaaacacct tgatgccgta agccgacaga cccgacgata
     1141 cgccgttgac gaccgccacc acgacgcctg caacggccga gacggtcgcc gagatcgccc
     1201 aggccatcgc gaatacgttc ttgacggaaa tgccgagcga ctgcgccacc tgctgattga
     1261 aggcggtggc gcgcatcgca agtccgtatt tggaaacccg gaagaaccac gccatgccga
     1321 ccatcatcgc caccgagacg acgaggctca tcacatagac ggtctggatt tgcaggccca
     1381 ggaaattcac cgactggctg gtgaacacgc gcgggaacgg ctgcggattg acgccgaaaa
     1441 tccatttcag cgaggcctga agcaccgtcg atagcccgat ggtgaccatg atcaccgaaa
     1501 tgatcggctc gccgatcatc ggccgcagga tcacgatctg tatggcgatg ccgaacacga
     1561 acatgaagac cagcgtcaac ggcatgccga cccagaacgg cacctgatat ttggtcaaca
     1621 gcgcccagca cacccaggcg ccgaccagca gcaattcgcc ttgcgcgaag ttcacgactt
     1681 gcgtcgcctt gtagatcagc acgaacgaca tcgcgaccac gccataaagc gtgccgacca
     1741 cgaggccgtt gaccagaagc tggatcagga actggatgtt catcgaaatt cgcccaaaat
     1801 gcaggtcgtc attgccgggc ttgacccggc aatccatcgt tcaaggaagt tggttttcac
     1861 gagcggatgg atgcgcgggt caagcccgcg catgacgacc gggtgtgccg aactctcgtt
     1921 cgtcatgccc gggcttgtcc cgggcatcca cgccttactt gagagctgaa aaaaagacgt
     1981 ggatggccgg gacaagcccg gccatgagtg cgtccgcgag gacgttttgt ctgaacggtg
     2041 caagtccgtt ctggggttgg tcgcaactcc ataatcgaag gt
//
LOCUS       DASWGM010000797         4430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2043026,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000797 DASWGM010000000
VERSION     DASWGM010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043026"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VGA15_22080"
     CDS             <1..1224
                     /locus_tag="VGA15_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX9590420.1"
                     /translation="AHLFGGEAVMMRLLCDVLTAQGFAVSAAIAGTSVCARTMTRHVH
                     GRIIRDGEEADAVRPLPVSALGTEDAITHGLRRAGLKTIGDVAFRERHEITARFGAGF
                     TTLLEHALGQGDAPISPRKPLPDYIVEKRFAEPIATEGVISVTLSRLAEMLVGAMAQQ
                     GKGARRLEAYFFRTDGAVRAIAVDTGQPVTRADMIDRLFRERLEALNDPLDPGFGFDL
                     IRLSASRTEIVVQQQRDLDANVHDNDELAALIDRIAARIGGRRVVVHLPQDTHIPERA
                     VQAVSAQHHLAAAAQAAWPARVDEEPPLRPLRLFERPEPIKVPFATVPDGPPHHFTWR
                     RATHAVVRVEGPERIAMEWWRQNGQALTRDYFRVEDEAGLRFWIYRDGLYGSELVTGE
                     GEPAPSNWFVHGLFA"
     gene            1221..>4430
                     /locus_tag="VGA15_22085"
     CDS             1221..>4430
                     /locus_tag="VGA15_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX9590421.1"
                     /translation="MNSVAYAEIGITTNFSFLRGGSHPQDYVHEASKLRLPAIGIADH
                     NTLAGVVRAYKELDNSELTYKPKLLIGSRLVFMDGTPDILVYPRDRAAYGRLCQLLTR
                     GKRGDDTEKGECHLKLDDLLEFAEGQLLAVMLPHRLNAAKAFDVLDRLQRSRADGVWL
                     AASLLYRGDDRRRLMRLYRIATTAGVPLLATNEVLYHHPARRPLQDVLSCIREKTTID
                     AIGRRLEANAERYLKPGHEMARLFRDLPEAITETMRFADRIDFSLDQLRYQYPDEPVP
                     PGKTAQRHLEDLTWAGAHKRFPIRIAPKTKKVLHKELRLIRKLKYAHYFLTVHDIVRY
                     ARSQKILCQGRGSAANSAVCYVLDVTSVDPTKVDLLFERFISKERLEPPDIDVDFEHS
                     RREEVMQYVYRRYGRHRAAIIATVIHYRPRSAIRDVGKALGLTEDVTAALADTVWGSW
                     GKGLNEMQVKQAGLDPQNAMVELAVELATELVEFPRHLSQHVGGYVLTQDRLDTYVPI
                     GNAAMDDRTFIEWDKDDVDALNMMKVDVLALGMLTCIRKCFDLIADHKGKRYELATIP
                     QDQKPVYDMLCRGESLGVFQVESRAQMNMLPRLKPRKFYDLVIEVAIVRPGPIQGDMV
                     HPYLRRRNGQEKVSYPSPSPAHGDKDELRRVLHKTLGVPLFQEQAMRIAIEAAKFTSE
                     EANGLRRAMATFRNVGTIGKFESKMVNNMIDRGYDPAFAKSCFDQIKGFGSYGFPESH
                     AASFAQLVYVSSWLKHFHPDAFCCGLLNSQPMGFYAPAQIVGDARNNGVEVREIDVSF
                     SHAQNTLEERSGNYHAVRLGFRQIDGFKWADPDEERLKQIQSSFRDAPLGADLRCALH
                     IGESKDSQMCNSTSEFVAAATPRNDGESPPAEDWAERIVAARTRRLFTSLEDFARDTG
                     LPKRALILLADADAFRSLGLDRRAALWAVRRLPDDVPLPLFEIATAREQPDESARPLP
                     LMPLPEQVVADYQTIRLSLKGHPMEFLRPMFTKERVIACEGVCHANDRRHVRCAGVVL
                     VRQRPGSAKGVVFMTLEDETGIANIVVWPKVMERFRKEVM"
BASE COUNT          834 a         1342 c         1436 g          818 t
ORIGIN      
        1 gcccatctgt tcggcggcga ggccgtgatg atgcggctgt tgtgcgacgt gttgaccgcg
       61 caaggctttg ccgtcagtgc ggccatcgcc ggcacctcgg tgtgcgcgcg gacgatgacg
      121 cgtcatgtcc atggccgcat catccgggac ggcgaggaag ccgatgcggt caggccgctg
      181 ccggtgtcgg cgctcggcac cgaggatgcc atcacccacg gcctgcgccg cgccgggctg
      241 aaaaccatcg gcgatgtggc ctttcgcgag cgacatgaaa tcaccgcaag gttcggcgcc
      301 gggtttacaa cgttgctgga acacgcattg gggcagggcg atgccccgat cagccctcga
      361 aaaccgctgc cggattatat cgtggagaag cggttcgccg aaccgatcgc caccgagggc
      421 gtgatatcgg tgacattatc ccggctggcc gagatgctgg tcggggcgat ggcgcaacaa
      481 ggcaagggcg cgcggcggct ggaagcttat ttctttcgca ccgatggcgc ggtgcgcgcg
      541 atcgcggtcg ataccggcca gcccgtgacc agagctgaca tgatcgaccg cctgtttcgc
      601 gagcggctcg aggccttgaa cgatccgctc gatcccggct tcggcttcga tctcattcgc
      661 ctgtccgcca gccgcaccga aatcgtcgtg cagcagcagc gcgatctcga cgccaatgtg
      721 cacgacaatg acgagttggc agccctgatc gaccgcatcg ccgcgcgcat cggcggccgg
      781 cgcgtggtcg tgcacctgcc gcaggacacc catattccgg agcgcgcggt gcaggctgta
      841 tcggcgcagc accatctggc ggcagccgcg caggcggcct ggccggcgcg ggtggacgag
      901 gagccgccgt tgcggccgtt gcggctgttc gaaagaccgg agcccatcaa ggtgccgttt
      961 gcgacggtcc ctgacgggcc gccgcaccat tttacatggc ggcgggcgac ccatgcggtg
     1021 gtgcgggtcg agggccccga gcgcatcgcc atggaatggt ggcggcagaa tggccaggcg
     1081 ctgacgcgcg attatttccg cgtcgaggat gaggctggat tgcggttctg gatttaccgg
     1141 gacgggcttt acggcagcga actcgtcacc ggggagggcg agccggcgcc ttccaactgg
     1201 ttcgtgcacg ggctgttcgc atgaatagtg tcgcttacgc cgaaatcgga atcacgacga
     1261 atttttcgtt cctgcgcggc ggctcgcatc cgcaagacta cgtgcatgag gcgagcaagc
     1321 tgcgtcttcc cgccatcggc atcgccgacc acaacacgct ggcgggtgtg gtgcgggctt
     1381 acaaggaact cgacaattca gagctaacat acaagccaaa gcttctgatc ggttcgcgcc
     1441 tcgtcttcat ggacggcacg ccggacattc tggtctatcc gcgcgaccgc gccgcctacg
     1501 gccggctatg ccagttgctg acccgcggca aacgaggcga cgataccgag aagggcgaat
     1561 gccatctgaa gctcgatgat ctcctcgaat tcgccgaagg ccagctgctg gccgtgatgc
     1621 tgccgcatcg cctcaacgcg gcaaaagcgt ttgatgttct ggatcgattg cagcgcagcc
     1681 gggccgatgg ggtgtggctt gcggcgagcc tgttgtatcg cggcgacgac aggcgccgtc
     1741 tgatgcggct ctatcgcatc gccacgacgg ccggcgtgcc tctgctggcg accaatgagg
     1801 tgctgtatca ccatcccgcg cgccgtccgc tgcaggatgt tctgagctgc attcgcgaga
     1861 agaccaccat cgacgccatc ggcaggcgtc ttgaagccaa tgcggaacgc tatctcaagc
     1921 ctggacacga aatggcgcgg ctgtttcgcg accttccgga ggcgatcacg gaaaccatgc
     1981 ggtttgccga ccgcatcgat ttttcgctcg atcagcttag atatcaatac cccgacgagc
     2041 cggtgccgcc gggcaagacc gcgcagcgcc atctcgaaga cctgacctgg gccggcgcgc
     2101 acaaaagatt tccgatccgg attgcgccca agaccaagaa ggtcttgcac aaggaactgc
     2161 gcctgatccg aaaactgaaa tacgcgcatt actttcttac cgtgcacgat atcgttcgct
     2221 atgcgcggag ccaaaaaatc ctctgccagg gccgggggtc ggcggcgaat tcggccgtgt
     2281 gctacgtgct cgatgtgacg tccgtcgatc cgaccaaggt cgatctgttg ttcgaacggt
     2341 tcatttccaa ggaacgcctc gagccgcccg acatcgacgt cgatttcgag cattcgcggc
     2401 gcgaggaggt gatgcagtat gtttaccgaa gatatggccg tcaccgcgcc gcgatcattg
     2461 ccaccgtcat tcattaccgg ccgcgcagcg cgatccgcga tgtcggcaag gcgctggggc
     2521 tgacggaaga tgttaccgcg gcgttggccg atacggtgtg gggaagctgg ggcaagggcc
     2581 tcaacgagat gcaggtcaag caggcggggc tcgatccgca aaacgccatg gtcgaactcg
     2641 ccgtcgagct cgccaccgaa ctggtcgaat tcccgcggca tctgtctcag catgtcggcg
     2701 gctatgtgct gacgcaggac cggctcgaca cctatgtgcc gatcggcaat gcggcgatgg
     2761 acgaccgcac cttcatcgag tgggacaagg acgacgtcga tgccctgaac atgatgaagg
     2821 tcgatgtgct ggcgctgggc atgctgacct gcatccgcaa atgttttgac ctgatcgcgg
     2881 atcataaggg caagcgttat gaactggcga cgatccctca ggaccagaag ccggtctacg
     2941 acatgctgtg ccggggcgaa tcgctcggcg tgttccaggt cgaaagccgc gcgcagatga
     3001 acatgctgcc gcgtctgaaa ccgcgcaaat tctatgacct cgtcatcgaa gtcgccatcg
     3061 tgcgtccggg cccgatccag ggcgacatgg tgcatcccta tcttcgacgc cggaatggcc
     3121 aggagaaggt gagctacccg tcgccttcgc cggcgcatgg cgacaaggat gaattgcgca
     3181 gggtgctgca caagaccctc ggcgtgccgc tgttccagga gcaggcgatg cggatcgcca
     3241 tcgaggccgc gaaattcacc tccgaagagg ccaatgggct gcgccgcgcg atggcgacgt
     3301 tccgcaatgt cggcaccatc ggcaaattcg aatccaagat ggtcaacaac atgatcgatc
     3361 gcggctacga tccggcattc gcgaaaagct gttttgatca gatcaaggga ttcggcagct
     3421 acggttttcc ggaaagccat gccgcgagct tcgcgcaact ggtctatgtc tcgtcatggc
     3481 tgaagcattt ccatcccgat gcgttttgct gcgggttgtt gaactcgcaa ccgatgggat
     3541 tttatgcccc ggcgcagatc gtcggcgatg cccgcaataa cggcgtcgag gtgcgcgaga
     3601 tcgatgtctc gttcagccat gcgcagaaca cgctggaaga gagaagcgga aactatcacg
     3661 cggtgcggct cggttttagg cagatcgacg ggttcaaatg ggccgatccg gacgaagagc
     3721 ggttgaaaca aattcaatcg tcattccggg atgcgccgtt aggcgcagac ctcagatgtg
     3781 cactgcatat cggggaatcc aaggattctc agatgtgcaa tagcacatcg gagttcgtcg
     3841 ctgccgcgac gccccggaat gacggtgaga gcccacctgc ggaagactgg gccgaacgca
     3901 tcgtcgccgc gcgcacccgc cgtctcttca cctcgctgga agatttcgct cgcgacacgg
     3961 ggcttcccaa gcgcgcgctg atcctgctgg cggatgccga tgcgttccgc tcgctcgggc
     4021 tcgatcgccg cgccgcatta tgggcggtgc ggcggttgcc cgatgacgtg ccgctgccgc
     4081 tgttcgaaat cgccaccgcg cgcgagcagc ccgacgagag cgcgcgtcca ttgccgctga
     4141 tgccgctgcc ggagcaggtg gtcgccgatt atcagaccat ccggctgtcg ctgaagggcc
     4201 atccgatgga atttttgcgg ccgatgttta ccaaggagcg cgtcatcgcc tgcgaaggtg
     4261 tctgccatgc caatgaccgg cgccacgtcc gctgcgccgg cgtggtgctg gtgcggcagc
     4321 gtccgggcag cgccaagggc gtcgtgttca tgacgctgga ggacgaaacc ggcatcgcca
     4381 atatcgtggt ctggccgaaa gtgatggagc gttttcgcaa ggaggtgatg
//
LOCUS       DASWGM010000798         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1777929,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000798 DASWGM010000000
VERSION     DASWGM010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777929"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VGA15_22090"
     CDS             complement(<1..366)
                     /locus_tag="VGA15_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX9590422.1"
                     /translation="MRNKNHDPNQMTGAAMIVRALIDHGVKHIFGYPGGAVLPIYDEI
                     FQQDDIEHILVRHEQGAGHAAEGYARSTGKPGVVLVTSGPGATNMVTPLTDALMDSIP
                     LVCITGQVPTHLIGNDAFQE"
     gene            complement(801..1736)
                     /gene="miaA"
                     /locus_tag="VGA15_22095"
     CDS             complement(801..1736)
                     /gene="miaA"
                     /locus_tag="VGA15_22095"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX9590423.1"
                     /translation="MSSVRENSNAVLIAGPTASGKSALALELAQKTGGVIINTDSMQV
                     YRDLRVITARPTPEEEAVVPHRLYGHIDAAVNFSAGSWVVDAAKVLAEARAQRRLPIF
                     VGGSGLYFKALTRGLSAVPPTPVAIREDVRARLERDGVEALHAELAQRDPVSAERLKP
                     RDRTRVARALEVVEATGRSLTDWHRDGLPPLLPQGQFRALFLSPDREQLYARIDSRFK
                     AMLLSGALEEVAALAARRLDPLLPAMKAHGVPALIRHLKGEITQDEAAVIGRADTRHY
                     AKRQFTWFRHQLPEFEWVRPDEARGLFTTVIPGRA"
     gene            1742..>1879
                     /locus_tag="VGA15_22100"
     CDS             1742..>1879
                     /locus_tag="VGA15_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX9590424.1"
                     /translation="MSLVATLICNPADPALDSTVVDGARAILPSPGRAQWLFDEVAVD
                     IP"
BASE COUNT          345 a          620 c          568 g          346 t
ORIGIN      
        1 ttcctggaac gcgtcattgc cgatcaggtg ggtcggcacc tgcccggtga tgcacaccag
       61 cgggatcgaa tccatcagcg cgtcggtcag cggcgtcacc atgttggtgg cgcccggccc
      121 cgacgtcacc agcaccacgc caggcttgcc ggtcgagcgc gcgtagccct cggcggcgtg
      181 accggcgccc tgctcgtgcc gcaccaggat gtgctcgatg tcatcctgct ggaaaatctc
      241 gtcatagatc ggcagcaccg cgccgcccgg atagccgaag atgtgcttga cgccatgatc
      301 gatcagcgcg cgcacgatca tggctgcgcc ggtcatctgg ttcggatcgt ggttcttgtt
      361 tctcatggct ggctccagat acgctttttg ctgcgcggct tcttcagttg ggtttttgga
      421 aaaaaaaagg gcccaagagg ccccatgcac accgcccgga tttggatggc ctcagccatc
      481 cccggcggtg cgcctgggta cgacaacgat aaggagcttg gtaattttat tacgcatatg
      541 accactcgaa cttctcgaag attgcgtaaa ccatagcgac catcccccaa aagtcaaggc
      601 aaggcgccac gaagcgttga gtttggagct tagcggggtt ccggtcgttc ggcgagggta
      661 aattttgcgc ttgccgccat gcgcggtccc tcgaccgtca ttccgggatg gggcgttcaa
      721 gtcggcaaca gccgacttga atggcccaga cccggaatct cgagattccc cgatgtgcaa
      781 ttgcacatct gaggttcgcg ctacgcgcgc cccggaatga cagtggtaaa caacccccgc
      841 gcctcgtccg gcctcaccca ttcgaattcc ggcagctgat gccggaacca ggtgaattgc
      901 cgcttggcgt agtgcctggt gtcggcgcgg ccgatcacag ccgcctcatc ctgcgtgatc
      961 tcgcccttca gatgccggat cagcgccggc acgccgtggg ccttcatcgc cggcagcagc
     1021 ggatcgagcc gccgcgccgc cagtgccgca acctcctcca gtgcgcccga caaaagcatc
     1081 gctttgaaac gcgagtcgat gcgcgcataa agctgctcgc gatcggggct aagaaacagc
     1141 gcgcgaaatt gaccctgcgg caacagcggc ggcaatccgt cgcggtgcca atcggtcagc
     1201 gaacgcccgg tggcctcgac cacttccagc gcgcgggcga cgcgggtgcg atcgcgcggt
     1261 ttcaagcgct cggcggatac aggatcgcgc tgcgcgagtt cggcatgcaa cgcctcgacg
     1321 ccatcgcgtt cgagccgcgc gcgaacgtcc tcacgtatcg cgacaggagt aggcggcacc
     1381 gccgagagtc cacgtgtcaa cgccttgaag taaagacctg acccgccaac aaaaatcggc
     1441 aggcggcgtt gcgcgcgcgc ttccgcaagc actttcgcgg catcgaccac ccagctaccc
     1501 gccgagaaat tcaccgcggc atcgatgtgg ccatagagcc ggtgcggcac gacagcctcc
     1561 tcttccggcg tcggccgcgc cgtgatgacg cgcagatcgc gatacacctg catggaatcg
     1621 gtattgatga tgacgccgcc ggttttttgc gcaagctcaa gcgccagcgc cgacttgccg
     1681 ctggcagtcg gccctgcgat aagcacggcg ttgctgtttt cccgaaccga actcacgcca
     1741 aatgtctctc gtcgccacgc tgatctgcaa tcccgccgac cccgcgctcg atagcacggt
     1801 ggtggacggc gcgcgcgcca ttcttccctc gcccggtcgg gcgcaatggc tgttcgatga
     1861 agtggcggtc gatattccc
//
LOCUS       DASWGM010000799         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2175818,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000799 DASWGM010000000
VERSION     DASWGM010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175818"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1507
                     /gene="guaB"
                     /locus_tag="VGA15_22105"
     CDS             11..1507
                     /gene="guaB"
                     /locus_tag="VGA15_22105"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX9590425.1"
                     /translation="MALVQGIQGIREAYTFDDVLLKPGLSDILPSEADIRSRVTRAIP
                     LNIPIIASAMDTVTEARMAIAMAQAGGVGVIHRNFDVDGQAAQVRQVKKFESGMVVNP
                     LTIGPDAMLSDALALMKDHGFSGIPVVTGAAKGVPGKLVGILTNRDVRFATDPRQKIS
                     ELMTHENLVTVREGVSQDEAKRMLHKHRIEKLLVVDEQYRCVGLITVKDMEKAVAHPL
                     ACKDAQGRLRVAAATTVGEDGFERTERLIDAGVDLIVVDTAHGHSSRVLAAVNRIKRL
                     SNAVQVIAGNIATMEGAQALIDAGADAIKVGIGPGSICTTRIVAGVGVPQLTAIMDAV
                     EAAKKANVPVIADGGIKYSGDLAKALAAGADIAMVGSLLAGTDETPGEVFLWQGRSYK
                     AYRGMGSVGAMARGSADRYFQQDIKDTLKLVPEGIEGQVPYKGPVGNVMHQLAGGLRA
                     AMGYVGARNLTEFHEKAQFVRITGAGLRESHVHDVTITRESPNYPGGA"
     gene            1792..>2258
                     /locus_tag="VGA15_22110"
     CDS             1792..>2258
                     /locus_tag="VGA15_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590426.1"
                     /translation="MVDWSDDRIAALSDQDLKNLLVNAEKKSVAEVVAQCRAELEKRD
                     AAKPRKASKPRTEVKAFEHDMSGQLATVGKEMADKYDLSEETAKANSVGVKGFKAHKL
                     LDAKGYAKLGGMQRDGSVAVDRYISYRRGKDIVSLSVFLLKDQPVESHEFHVIA"
BASE COUNT          465 a          676 c          729 g          388 t
ORIGIN      
        1 ggagttggtg atggcgctgg tgcagggaat tcagggaatc cgcgaagcct atacgttcga
       61 cgacgtgctt ctgaaacccg gcctttcgga tatcctgccc tcggaggccg atatccgctc
      121 ccgcgtcacc cgcgccattc cgctcaacat cccgattatt gcgtctgcga tggataccgt
      181 caccgaagcg cgcatggcga tcgcgatggc gcaggccggc ggcgtcggcg tcatccaccg
      241 taatttcgac gtcgacggcc aggccgccca ggtgcggcag gtcaagaaat tcgaatccgg
      301 aatggtggtc aatccattga ccatcggtcc cgacgcgatg ctgtcggacg cgctggcgct
      361 gatgaaggat cacggctttt ccggcattcc cgtggtcacg ggcgcggcca agggcgtgcc
      421 gggcaagctg gtcggcattc tcaccaaccg cgacgttcgt tttgcgaccg acccgcgcca
      481 gaaaatctct gaactgatga cgcacgagaa tctcgtgacg gtgcgcgaag gcgtcagcca
      541 ggacgaggcg aagcggatgc tgcacaagca tcgcatcgaa aaactattgg tggtcgacga
      601 acaatatcgc tgtgtcggcc tgatcaccgt gaaggacatg gaaaaggcgg tcgcgcatcc
      661 cttggcctgc aaggatgcgc aaggaaggct gcgggtcgcc gccgctacca ccgtcggcga
      721 ggacggtttt gagcgtaccg agcggctgat cgacgccggg gtcgacctga tcgtggtgga
      781 taccgcgcac ggccattcct cgcgcgtgct agccgccgtc aaccgcatca agcggctctc
      841 caatgcggtg caggtcatcg ccggcaatat cgccaccatg gagggtgcgc aggcgctgat
      901 cgacgccggc gccgacgcga tcaaggtcgg catcggtccg ggttcgatct gcaccacccg
      961 gattgtcgcc ggtgtcggcg tgccgcagct caccgcgatc atggatgcgg tcgaagccgc
     1021 gaagaaagcc aatgtgccgg tgatcgccga cggcggcatc aaatattccg gcgatctggc
     1081 caaggctctg gccgccggcg ccgacatcgc catggtgggc tcgttgctgg ccggcaccga
     1141 cgagaccccg ggcgaggtgt tcctgtggca gggccgctcc tacaaggcct atcgcggcat
     1201 gggctcggtg ggggcgatgg cgcgcgggtc cgccgaccgc tacttccagc aggacatcaa
     1261 ggacacgctc aagctggtgc cggaaggcat cgagggccag gtgccctaca agggcccggt
     1321 cggcaacgtc atgcatcagc tcgccggcgg cctgcgcgcg gcgatgggct atgtcggcgc
     1381 gcggaacctg accgagttcc acgagaaggc gcagttcgtg cgcatcaccg gtgcggggct
     1441 gcgcgaaagc cacgtccacg acgtcaccat cacgcgcgaa agcccgaatt accccggcgg
     1501 ggcgtagagc gttttcaagc gaagcatgcc ctcggacttg atccgggggg tggagaccgg
     1561 ttcgcgtcaa gaaaacgcgt caaatcaaga atctagagcc ccgtttcgat tctatcgaaa
     1621 cggaaaaggc tctacctttg ccgggccaaa ttgaccacca caaggctggc gaaacgcggt
     1681 actgttggcg ccacggatgg ctgcgctgcc tggaaatcca tgcccgcgat tgtgccgcgg
     1741 tcttcaagag atttcacctc aacatcattc gaatagagaa gttgaaacat gatggtcgat
     1801 tggtcggacg acaggattgc tgcactttca gatcaggatc tgaagaacct gctggtcaat
     1861 gcggagaaga aatccgtcgc cgaagtcgtc gcgcaatgca gggccgaact ggaaaagcgg
     1921 gacgccgcaa aaccacgcaa ggcatcgaag ccgcgcaccg aagtgaaagc gttcgagcac
     1981 gacatgtccg gtcaactcgc caccgtcggc aaggagatgg ccgacaaata cgacctgtcg
     2041 gaagaaaccg ccaaagcgaa ctcggtgggc gtgaaaggtt tcaaggcgca caagctgctg
     2101 gacgccaagg gttatgccaa attgggcggc atgcagcgcg acggctcggt cgcggtcgac
     2161 cgctatattt cgtaccggcg cggcaaggac atcgtctcgc tgagtgtctt cctgctgaag
     2221 gatcagccgg tcgaatccca tgagtttcat gtgatcgc
//
LOCUS       DASWGM010000800         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1777950,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000800 DASWGM010000000
VERSION     DASWGM010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777950"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGA15_22115"
     CDS             <1..241
                     /locus_tag="VGA15_22115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590427.1"
                     /translation="DPLSKSRWSIFERNGPVSPGKRASYHGSAQIPQLGIALRQLALA
                     FAEMAKLSPGALYHTAPQTAKHLGVVNHGIRSRVP"
     gene            368..838
                     /locus_tag="VGA15_22120"
     CDS             368..838
                     /locus_tag="VGA15_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX9590428.1"
                     /translation="MNVDDAGSGAPGAGRQPALTGCPWHDVNEMLQAAGLRPTRQRMA
                     LGWLLFGKGARHLTAEMLYEEATHAKVPVSLATVYNTLNQLTDAGLLRQVSVDGTKTY
                     FDTNVSAHHHFYLENNHELVDIPDPHLMLQKMPEVPEGYEISRVDMVVRLRKKR"
     gene            complement(1031..1558)
                     /locus_tag="VGA15_22125"
     CDS             complement(1031..1558)
                     /locus_tag="VGA15_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX9590429.1"
                     /translation="MVLRRICSVMVLAGSMLGGSVSPGFSAKDSPVTASGLPVPRYVS
                     LKSDHVNVRAGPTKDNDVAWVYTRSGLPVEITAEFENWRRVRDSEGAEGWVYHSLLSG
                     RRTAVITMKTKDEFATLYDRADPTSAVAARLQAGVVAQVKHCAAGWCRVAGDGFDGWI
                     EQQRLWGVYADEKVD"
     gene            1913..>2601
                     /locus_tag="VGA15_22130"
     CDS             1913..>2601
                     /locus_tag="VGA15_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590430.1"
                     /translation="MSVKKKPLVVVTRKLPDSIETRMRELFDARLNLDDTPLTPEQIA
                     DAVRSADILVPTVTDVITEELLRQPDCKVRLIANFGNGVDNIDVIAAHARGITVTNTP
                     KVLTEDTADMTMALILAVPRRLIEGASILTEGKNWPGWSPTWMLGHRIGGKRLGIIGM
                     GRIGQAVARRARAFGLQIHYHNRRPVAPRIAEELGATYWESLDQMLARMDIISVNCPH
                     TPATFHLVSARR"
BASE COUNT          547 a          819 c          764 g          471 t
ORIGIN      
        1 tgaccctctc tcaaaatccc gatggagcat tttcgagcga aatggacccg tttcgcctgg
       61 gaagcgcgcc agttatcatg gatcggcaca aataccacag ctcggaattg ccctgcggca
      121 attggcgctc gcctttgccg agatggcaaa gttaagtccg ggcgctctct atcatacagc
      181 cccgcagacg gcaaagcacc tcggcgtggt aaaccacggc atccgctccc gggttcccta
      241 gtggccttgg accggtctag ttgcgaaaag cttgcatcta accagtttct ttcttatatt
      301 ccaccacgat agtgctgaga cagcgagtgc ggtgcctgca gtggaaacga gcaatgacgg
      361 ttcgagtttg aatgttgacg atgccggctc cggggctccc ggtgcgggac gtcaaccggc
      421 tttgaccggt tgcccgtggc acgatgtcaa tgaaatgctg caggcggccg gactgcgtcc
      481 gactcgtcag cggatggcgc tcggctggct gttgttcggc aagggcgcac gccatctcac
      541 cgcggaaatg ctttacgagg aagcgaccca cgccaaggtt ccggtgtcgc tcgccacggt
      601 ctacaacacc ctcaatcagc tcaccgatgc gggcttgctg cgtcaggtca gcgtcgacgg
      661 caccaagacc tatttcgaca ccaacgtgtc ggcgcatcac cacttctatc tcgaaaacaa
      721 tcacgaactg gtcgacattc cggatccgca tttgatgttg cagaagatgc cggaagtgcc
      781 cgaaggctac gagatctccc gggtcgacat ggtggtccgg ctgcgcaaga agcgctgatt
      841 ttcacgcctc gcgtcgtccc ggcggaacgc cgggacccat aacccctgtg attgatgatt
      901 aaagacggcg tcggccgcag tgccgaaaac gcgaatcctc agcgtatgga tcccggctcg
      961 cgttcgcttg cgctcacttg gccgggacga ctcgtggaaa atggatccgc aggacgtcga
     1021 cgttgaagaa ctaatccacc ttctcatcgg cgtagacgcc ccacagccgt tgctgctcga
     1081 tccagccatc gaagccgtcg ccggcaacac ggcaccagcc ggccgcgcaa tgcttgacct
     1141 gggctaccac cccggcctgc aggcgggctg caaccgcgct ggttggatcc gcgcgatcgt
     1201 acagcgtcgc gaattcgtcc ttggttttca tggtgataac cgcggtgcgg cggcccgaca
     1261 gcagcgaatg atagacccag ccctcggcgc cttcggaatc acggacccgg cgccagttct
     1321 cgaattcagc ggtgatttca accggcaggc ccgagcgggt gtagacccag gcgacgtcgt
     1381 tgtccttggt cgggccggcc ctgacgttga cgtgatcgga tttgaggctg acgtagcgcg
     1441 gcaccggcaa cccgctggcg gtgacgggcg aatcctttgc cgaaaagcct gggctcaccg
     1501 acccacccag catggacccc gcgagcacca tcacagaaca aatacgcctc aacaccatca
     1561 actcgtctcc tgccgagata ccaactcaaa tcgctggagt tgcaaccgca aatgccgacc
     1621 ccagaagccc cctaccgcgt cccggcttcc ctcggcttgt catttggggt tcttgttttg
     1681 gcccggcctt ctgctagaga ggacggaacg atgcgaaaca agacgacccc gaattttcct
     1741 tggaaacaaa agggaaatat cggggaaccc aaggaaacct agagcaagcc tggcgagcaa
     1801 acaccgggac cacgcggcag ccgcagtgtc gaacaaccgg gttaatgagg cctgaacggc
     1861 gaagcctttg acaacctaaa ggttggcgag taacgcctca tgagagcagg acatgtcggt
     1921 taagaaaaaa cctctggttg tcgtgacgcg caagctgccg gactcgatcg agacccggat
     1981 gcgcgagctg ttcgacgcaa ggctcaatct cgacgatacg ccgttgacgc cggaacagat
     2041 cgcagacgcg gtccgctcag ccgatatcct ggtgccgacc gtcaccgacg tgatcaccga
     2101 agagctgctc aggcagcccg actgcaaggt gagactgatc gccaatttcg gcaacggcgt
     2161 cgacaatatc gatgtcatcg cagcacatgc gcgcggcatc accgtcacca acacgccgaa
     2221 agttctgacc gaagacaccg ccgacatgac catggcgttg atcctggcgg tgccgcggcg
     2281 gctgatcgaa ggcgcctcga ttctcaccga aggcaagaac tggccgggct ggtcgccgac
     2341 ctggatgctc ggccaccgta ttggcggaaa acggctcggc atcatcggca tgggccgcat
     2401 cggccaggcc gtggcgcgcc gggcgcgagc cttcggcctg cagattcact accacaaccg
     2461 ccgtccggtc gcgccgcgga tcgccgagga attgggcgcg acctattggg aaagcctcga
     2521 ccagatgctg gcgcggatgg acatcatctc ggtgaactgc ccgcacacgc cggcgacttt
     2581 tcacttggtc tcggcgcgtc g
//
LOCUS       DASWGM010000801         5011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2318933,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000801 DASWGM010000000
VERSION     DASWGM010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2318933"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VGA15_22135"
     CDS             complement(<1..1630)
                     /locus_tag="VGA15_22135"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX9590431.1"
                     /translation="MHQRTIRFWLTWLVVGCVLPATLGSAFLFTISYQQQRAILERNT
                     IATARALMQAIDAELFGAQSALQVLAASHRLASGDLASFYLQASEALPNIGGNYIAIT
                     DSTGQQRLNTLKPFGDPLPQVLPSAKMRRVFENGKPAISDFFVVPEVGQSAITLEVPV
                     FSNGKVIYSLAMAIFADRLADILHRQNIPPDWITAVFDSTGTIAARTHSPQRFVGGNG
                     SAGFLLRSANRAEGITEADSLEGTPVLSAYNRSPRSGWAVAIGIPRTSLTGNPWRSLG
                     FNAAVTLALLALSIWMAKIISVRINRSIRSLHVPALALGTPELLSIPDNEIVEVNDVG
                     QALMKASRMIRERVVEREQGKLATREMILAKQIADRASRAKSEFLASMSHELRTPLNA
                     ISGFAQLLGRSGDTLDREKRIRYAQTIVEASTQLEKLIDDVLDMARFEPGDVEVNCEV
                     LDCLEVMTEVSRSLEVSARKKGVLFTVDVSGNLPSIVADRGRLIQVLLNLGSNAIKYN
                     VEGGWVLLTAFACDDVVRFIVRDTGKGIPAERHGE"
     gene            2374..3882
                     /locus_tag="VGA15_22140"
     CDS             2374..3882
                     /locus_tag="VGA15_22140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9590432.1"
                     /translation="MRFTSLVIELIRARPRLVVWLVVLLQAALWLMLPLLLYGSPPGD
                     LATVLAYGREYQVGTDLGPPLAFWLGDIAFRAAGNHMFGVYLLAQLCSVATFWTLFLL
                     ARAVVGGQQAVLAVLLTMTVTAFGSPGVEFGPLVLARPLWALLLLHSWQLIGQGRRSA
                     WFAWSIEAGLLLLTTSAALGLLTLVAGFAVATLRGRRTLTSFDPLFALLVIVVLVLPY
                     LIWLIRADTLALPQWPAIADLSDRALHWAELLGGLLLAISGIVLLVVINSDWFGRNPE
                     EAPIIYRPPVEPLARDFVYFFAIAPALAGSLISGLFNIDHVAGGAGIALLMSGLAVIV
                     ATGDLVHLRRQRVLRLVWAAAVVAPALAVILTALLLPWTGGAEVATSLPATAIAHFFG
                     DSFERRTNRPLPAVTGDAQLASLIALDAARPHLLLDAAPERTPWLTLAKFNETGGVVV
                     WRASDTVGTPPADIVRRFPGLVPEVPRAFEWPVTGRQPLLRIGWAIVRPKTP"
     gene            complement(4183..5001)
                     /locus_tag="VGA15_22145"
     CDS             complement(4183..5001)
                     /locus_tag="VGA15_22145"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9590433.1"
                     /translation="MTPDSAPTVQQLLAFYLEAGVDCALTEAPVNRLSDPDIVPLSSE
                     TAEPKPVRTIPAAIPATRGEAAPAPEAAILSARDAARTAPSLEVLRALLENFDGCALK
                     STATRLVFADGNPQARIMFVGEAPGREEDIEGLPFVGRSGKLLDRMIAAIGLDRSSAY
                     IANVIPWRPPGNRTPTPQETQICLPFIQRQIELVNPDVLVTLGNPSTQTLLSTREGIM
                     KTRGRWFDYDTGTRMIRALATFHPAYLLRSPSYKRMAWQDLRSIAKALEHSSSG"
BASE COUNT          887 a         1528 c         1598 g          998 t
ORIGIN      
        1 tttcgccgtg acgctcggca ggaattccct tgccggtatc cctgacgatg aagcgcacga
       61 cgtcgtcaca ggcaaacgcg gtcagcaaca cccatccgcc ctcgacattg tatttgatcg
      121 cattgcttcc aaggttcagc agaacctgaa tcagccgccc gcggtcggcg acgatggagg
      181 gcaggtttcc ggagacatcg accgtaaaga gcacgccttt ctttctcgcc gaaacctcca
      241 gcgagcggct cacttcagtc atgacttcca gacaatccag aacctcacag ttcacctcga
      301 catcgcctgg ttcgaaacga gccatgtcga gaacgtcgtc aatgagtttt tccagctggg
      361 tgctcgcttc cacgatggtc tgcgcatagc gaatccgttt ttcgcggtcg agcgtatctc
      421 cggaccgacc cagcaattgg gcaaagcccg agatcgcatt cagcggtgtc cgtagctcgt
      481 ggctcatcga tgcaaggaac tccgacttcg cgcggctggc tctgtcggcg atttgctttg
      541 ccaggatcat ttcccgggtg gccaatttgc cctgctcgcg ctccaccaca cgctccctga
      601 tcattcgcga ggccttcatc agcgcctggc cgacgtcatt gacctcgacg atttcgttgt
      661 ccggaatcga cagcaattcc ggcgttccga gcgcgagggc cgggacgtga agcgaccgga
      721 tcgaacgatt gatccggaca ctgatgattt ttgccatcca gatgctcaag gcgagcagcg
      781 caagggtcac ggcggcgttg aaacccagtg accgccacgg gtttccggtc aggctggtcc
      841 tcgggatgcc gatggcgaca gcccagcccg accgtggtga ccggttgtac gcgctcaaca
      901 ccggggtgcc ctcgagcgag tcggcctcgg tgataccctc ggcgcggttg gctgacctca
      961 gcaggaagcc cgccgaaccg tttccgccca cgaaacgttg cggggagtgg gttcgcgcgg
     1021 cgatcgtgcc ggtgctgtcg aatacggcgg ttatccaatc cggcggaata ttctgtcggt
     1081 gcaggatatc ggcaaggcga tcggcaaaga tggccatggc gagagagtag atcaccttcc
     1141 cgttcgagaa gaccggcact tccagggtga tcgcagactg cccgacttcg ggaacaacaa
     1201 aaaaatcgga gatcgccggt ttgccgttct cgaacacccg ccgcattttg gcggagggca
     1261 gcacctgagg cagcggatca ccgaatggct tgagcgtatt gagacgctgc tgcccggttg
     1321 aatcggtaat ggcgatgtag ttgcctccga tattcggcaa tgcctcgctc gcctgcagat
     1381 aaaaactcgc cagatcaccg gaggccaaac gatgcgatgc tgcgagcacc tgcagcgccg
     1441 attgcgcacc gaacaactcg gcatcgatcg cctgcatcag cgcgcgggcg gtcgcgatcg
     1501 tgttgcgttc gagaattgcc cgctgctgtt gataggaaat ggtgaacaga aacgcagatc
     1561 ccagcgtcgc cggcagaacg caaccgacga ccagccaggt gagccaaaat cgaatcgttc
     1621 tctgatgcaa gtgagattcc ttgacacggc ctcgatcacg gggcctcgct atgagaaaaa
     1681 cgcacggcaa agcacaaacg cggatcaacc gggaccacgg cggagtcatc ccgcctcgtg
     1741 gcgatgatca accgaggtcg cgcaacgcac tatccgacgc ttactgtgaa aattaccggc
     1801 cgttacaggg ccgccgacag gctgggtcag ccgcatcgca agccgtcgac gcatcttccg
     1861 caaatcaatc ggctctcgtg aattgtgaga cgcgggaaac gcaatgtcaa ttgctcgaac
     1921 gagtcatcgt cagcccgagc gcttgttccc gcagttgcac aagtggcaca tgatgattgt
     1981 tcgcgtcggc gagcagaagg acgtcctgac gcgaccgaat atgtcacgcg gatggatcac
     2041 taaaaattcg gcacggcgga agtgaagcgc ggcatggcga agtcgacctc gtgccgcgca
     2101 ttcgccgcgc tgaacgaaaa cgaaagtgtc tcgtcaagtg tgacggctaa ggtgttcggc
     2161 tctcggagag aaagctgatt tgcggcaatc acgatcttgt tcggattgca actcatgtcg
     2221 tgccggatgg attgggaagc ggggcgcctg cgcgtcgaca cgggatcagt ctgttgcctc
     2281 agcggctcac gcgctttatc aacgttcgag atcgaacagg gccggccaat gggctcgagt
     2341 caataggttc gacttgggca tttcggacga ttcatgcgtt ttacctccct ggtcatcgaa
     2401 ctgatccgcg cccggccgcg gctggtggtc tggcttgtgg tgctgctgca ggcggcgttg
     2461 tggctgatgc tgccgctgtt gctgtacgga agtcctcccg gcgatcttgc gacggtactg
     2521 gcctatggcc gggaatatca ggtgggaacc gatctcggcc cgccgctggc attctggctc
     2581 ggcgatatcg cgttccgcgc cgcgggcaat catatgttcg gcgtctatct gctggcgcag
     2641 ctttgctccg tcgcaacgtt ctggaccttg tttctcctgg cacgcgcggt tgtcggcgga
     2701 caacaggcgg tgctggcggt cttgctgacg atgacggtca cggcgttcgg ttcgcccggc
     2761 gtcgagttcg gccccctggt gctcgcgcgc ccgctctggg cgctgctgct gctgcattcc
     2821 tggcaactga tagggcaggg gcgccgcagc gcgtggttcg catggtcgat cgaagccgga
     2881 ctcttgctgc tgacgacctc agccgcgctc ggcctgttga ctttggtcgc cggatttgcg
     2941 gtcgcaacct tgcgcggacg gcgcacgctg acatcgttcg atccgctgtt cgcgctgctg
     3001 gtgattgtcg tgctggtgct gccctatctg atctggctga tccgcgccga tacgctggcg
     3061 ttgccgcaat ggccggcgat cgcggacttg agcgaccggg cgctgcactg ggccgagctg
     3121 ctcggcggct tgctgctggc aatttccggc atcgtgctgc tggtggtgat caactcggac
     3181 tggtttggcc gcaacccgga ggaggcgccg atcatctatc ggccaccggt cgagccgctg
     3241 gcccgggatt tcgtttattt cttcgccatc gcacccgcgc tggcgggaag tctgatctcc
     3301 ggacttttca atatcgatca tgtcgcgggc ggggcgggca tcgcgctatt gatgtcggga
     3361 ctggcggtca tcgtggcgac cggcgacctg gttcatctgc ggcgccagcg cgtgctgcgt
     3421 ctggtctggg ccgctgccgt cgtcgcgcct gcgctcgcgg taatcctgac cgcgctgttg
     3481 ctgccctgga ccggcggcgc cgaggtggcg acgtcactgc ccgcgaccgc gatcgcgcat
     3541 ttttttggcg acagttttga gcggcgcacc aatcgcccgc tgcccgcggt gaccggcgac
     3601 gcacagcttg cgagtttgat cgcattggac gccgcgcgtc ctcatctgct gcttgacgcg
     3661 gcaccggaac gaacgccttg gctgacattg gcgaaattca atgaaaccgg cggtgtcgtg
     3721 gtctggcgcg catccgacac ggtcggcaca ccgccggccg acatcgtgcg gcgatttccc
     3781 ggcctggtgc cggaggtgcc gcgggcgttc gagtggccgg tgaccggccg gcagccgctg
     3841 ttgcgaatcg gctgggccat cgtgcggccg aagacgccgt aaaatcgttt gcgagcaagg
     3901 catgcggtcg gtttccttta cctctcccag agggagaggt cggatcgcct cggcgatgcc
     3961 aacgggtcgc gcgaatgcgc gcccgatgac aggctccgca tcgtccgatg caatccgggt
     4021 gaggggttac ggcctaacga tagatcgtaa cccctcaccc caaccctctc ccatcgaagt
     4081 cggatttatc cgacttcgac gatttgggat gtccaactcg ggtaaacccg agttggatgg
     4141 gagagggagc gcgcttccac tgccgcgatg tagaaccgag cttcagccgc tggaagagtg
     4201 ctccagcgcc tttgcgatcg aacgcaaatc ctgccacgcc atccgcttgt aggacggtga
     4261 acgcaacaga tatgccggat ggaaggtggc gagcgcgcgg atcatccgcg tgccggtgtc
     4321 ataatcgaac caacgccctc gggttttcat gatgccctcg cgggtcgaca gcagggtctg
     4381 ggtcgaggga ttgccgagcg tgaccagcac gtccgggtta accagttcga tctgccgctg
     4441 gatgaacggc aggcagattt gcgtctcctg cggcgtcggc gtgcggttgc ccggcggccg
     4501 ccatggaatg acattggcga tgtaggcgct ggagcggtcg agcccgatcg ccgcgatcat
     4561 ccggtccagc aacttgccgg aacgtccgac gaacggcagc ccctcgatgt cctcctcgcg
     4621 cccgggggct tcgccgacaa acatgatgcg cgcctgcggg ttgccgtcgg cgaataccag
     4681 gcgtgtcgcg gtggatttca gcgcgcaacc gtcgaaattc tccaacaggg cgcgcagcac
     4741 ctcgagcgaa ggtgccgttc gcgccgcgtc ccgcgccgac aggatcgcgg cctccggcgc
     4801 tggcgctgcc tcgccgcgtg tcgccgggat cgcggcagga atggtcctga ccggtttggg
     4861 ctccgcggtt tcgcttgata ggggaacgat gtcgggatcg gacagccggt tgaccggcgc
     4921 ctccgtcaac gcgcagtcga ccccggcctc cagataaaag gccagcaatt gctgaacagt
     4981 gggggcgcta tcaggcgtca tggttccatg t
//
LOCUS       DASWGM010000802         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2607807,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000802 DASWGM010000000
VERSION     DASWGM010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607807"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1810
                     /locus_tag="VGA15_22150"
     CDS             <1..1810
                     /locus_tag="VGA15_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9590434.1"
                     /translation="LDVLEQGDPALEQNGTRIHDQKIATALGPRWIAWREGLVRIDAG
                     RPAEMQCVGRDVTDRTETERALAEARDLADAANRAKSRFLAMASHEIRTPLNGIIGMS
                     GLLLDTPLTPEQMTYAKAVKTSGDALLSLIEELLDYSKIEAGKIDLEHRPFALAALIE
                     DITELLAPRAQARKLEIAAYVDERLPMEVVGDAARLRQVLLNLAGNAIKFTSTGGVAL
                     IVEPGISPDEISFLVRDTGIGIAPEAQRRIFREFEQADEHIARSYGGTGLGLSISERI
                     VRRMGGRITLESQPGAGSTFEVSIPLAPSDDAAGEQRTFAAPDLTAQSIMLVAPQSIE
                     ASLIARRLQRWGGQTCMVSDVKVAQALLPERSWHAVLLDQALGTADVELLGHAARPHA
                     TQRIVMLTPAARHELQLSSASSAASAFTGYLVKPLRAASLAARLTMTPEIPAPNLAEI
                     APVEASDPTGTPMTAPVQGLSVLVAEDNEINALLMRSLLTRLGHHAVVCSNGEAAVES
                     WLSARSGGAPYDLVLMDIQMPQLDGIETTKRIRGLEAGHPGRQTPILALTANTLAEDR
                     YACFEAGMDGFLIKPLDREKLADALAGLAAARHLAA"
     gene            2052..3023
                     /locus_tag="VGA15_22155"
     CDS             2052..3023
                     /locus_tag="VGA15_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9590435.1"
                     /translation="METTDTMIPSGDAGIQLFVRNKHPAGRETSPDKILLFVHGATYP
                     AETAFDLPIEGVSMMDLIAARGYDVYLVDVRGYGRSTRPAEMSQPPAANKPIVSTKVA
                     AHDLGIAVDYILRKRKVSKINVMGWSWGTSIAGSYTSEHNDKVNRLVLYAPQWIRNEP
                     PAPAAANVPPLGAYRLVSKDSAKVRWLKDVPADKQADLIPPGVFDAWANATWATDPES
                     SKQNPPMLRAPNGVVEDSVNYWSAGKALYDPGKITVPTLLLHAEWDADLPSYLAQGYF
                     AQLKNTPYKRLIELSEGTHTVMMEKNRMQFFRELMGFLDEEKPLALK"
     gene            3333..3965
                     /locus_tag="VGA15_22160"
     CDS             3333..3965
                     /locus_tag="VGA15_22160"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9590436.1"
                     /translation="MKTLQIVCAVLLLVLGCSQASAEDSRELVSALKQGGHVIVFRHG
                     ATDDSQKDIYPLNFDDMKAQRQLSEKGRDMARQIGTAIKKLGIPIGEIYTSRLNRAIE
                     TGKLLSDKEVKPLDALTDSGAGSASAMANPAGGNAKAGLALRELVNAAPKAGTNTFIV
                     THKTNIADAFGKEASDVQEGEAFVYRSSGSAPAAFVTRVKPAFWIDQAAN"
     gene            4103..5056
                     /locus_tag="VGA15_22165"
     CDS             4103..5056
                     /locus_tag="VGA15_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9590437.1"
                     /translation="MSMQNGEFAQRFIEMLRPENVGNAAQTAITWLKPPPAPIGSKPL
                     RLANFPATLGRLGALEVRLAQEKRDVRRAQKLRYHVFYKDGAAIADAATLLARRDKDA
                     FDKICDHLMVIDHAAKPSIGGKQPVVGTYRLLRQDVAESHGGFYTEDEFDIAGLVERH
                     AGLRFLELGRSCVLPRYRNKRTVELLWHGIWSYVRQHRLDVMIGCASFEGTDPDRLKL
                     PLSFLHHYAQAPEHWRAKAHPSRRVEMNRMAKEAINPKAALHELPPLIKGYLRLGAYI
                     GDGAVIDHQFGTTDVLIVMPVSAINARYIEHFGVDASRHAA"
     gene            complement(5290..>5430)
                     /locus_tag="VGA15_22170"
     CDS             complement(5290..>5430)
                     /locus_tag="VGA15_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX9590438.1"
                     /translation="ATALAIWERTNLANLEENILPTRPRATLILKKGADHMVETVALR
                     RL"
BASE COUNT         1107 a         1682 c         1670 g          971 t
ORIGIN      
        1 gctcgatgtt cttgagcaag gcgatccggc actggaacag aacggtacgc ggatccacga
       61 ccagaaaatc gcgaccgcgc tcgggccgcg atggatcgca tggcgcgaag gcctggtccg
      121 tatcgatgcc ggccggccgg cggagatgca atgcgtcggc cgcgatgtca ccgaccgcac
      181 cgagaccgaa cgcgcgcttg ccgaggcccg cgaccttgcc gacgccgcca accgcgccaa
      241 gtcacggttc ctcgcgatgg catctcatga gattcgcacg ccgctcaatg gcatcatcgg
      301 catgagcggg ttgctgctgg atacgccgct gacgccggag cagatgacct acgccaaggc
      361 ggtgaaaacc tcgggcgacg ctttgctgtc gctgatcgag gagctcctgg attattccaa
      421 gatcgaggcc ggcaagatcg atctcgaaca tcgcccgttc gcgcttgccg cattgatcga
      481 ggatatcacc gaactgctgg cgccacgggc gcaagccaga aagctcgaga tcgcagccta
      541 tgtcgacgag cggctgccga tggaagtggt cggcgacgcc gcacggctgc ggcaggtgct
      601 gctcaacctc gccggcaacg cgatcaagtt cacctcgacc ggcggcgtgg cgctgattgt
      661 cgagccgggg atctcgcccg atgaaatcag cttcctggtg cgcgataccg gcatcggcat
      721 cgcgcccgag gcacagcggc ggattttccg cgagttcgaa caggccgacg aacacatcgc
      781 ccggagctat ggcggcacag gcctcggcct gagcatctcc gagcgcatcg tcaggcgaat
      841 gggcggccgc atcacactgg agagccagcc gggtgccggc tcgacattcg aggtgtcgat
      901 tccgctcgcc ccctccgacg acgctgctgg cgaacaacgg acgtttgcgg cgccggattt
      961 gacggctcaa tcgatcatgc tggtcgcgcc ccagagcatc gaggcatcgc tgatcgcccg
     1021 gcggctgcaa cgctggggcg gtcagacctg catggtttcg gatgtcaagg tggcgcaggc
     1081 cctgttgccg gagcggtcct ggcacgccgt tctgctcgat caggcattgg gcactgccga
     1141 tgtcgaattg ttgggtcacg ccgcgcgccc gcacgcgacg cagcggatcg tgatgttgac
     1201 gccggcggcg cgacacgagc ttcagctgtc ctccgcctcc tcggccgcct cggctttcac
     1261 gggctatctg gtcaaaccgt tgcgcgcggc atcgcttgcg gcccgcttga cgatgacccc
     1321 ggaaattcca gcccctaacc tcgccgaaat tgccccggtc gaggcatctg atcctaccgg
     1381 gacgccgatg accgcgccgg tgcaaggtct ttccgttctg gtggcggagg acaatgagat
     1441 caacgcgctc ttgatgcgtt cgctgttgac caggcttgga catcacgccg tcgtctgcag
     1501 caacggcgaa gcggcggtgg aatcatggct ttcagccaga tccggcggcg caccgtatga
     1561 tctggttctg atggacatcc agatgccgca actcgacggc atcgaaacca ccaagcggat
     1621 ccgcggcctg gaggctgggc acccgggccg ccagacgccg atcctcgccc tcacggcgaa
     1681 tacgctggcc gaggaccgct acgcttgctt tgaggccggc atggacggtt tcctgatcaa
     1741 gccgctcgat cgtgaaaagc ttgccgacgc gcttgccggt ttggctgccg cgcggcatct
     1801 cgccgcctga aggctgcacg acgcgccgac cgctcgcgcg atactcttga cgctgccggc
     1861 gattggaccg agcatgtgag agctacccgg tccgctcgag cggactggaa agcaggctgc
     1921 caacgcgcgg cccaaaaaag caaaaacgtc ccgggaggac ttcaatgacc aacaggtttt
     1981 tacagatatc tctggcgtgt cttgtcgcaa gcaccttcgc gagcgcagcc gcacaggcga
     2041 aggctccaaa gttggaaaca acagatacca tgatcccgtc aggcgatgcg ggtatccagt
     2101 tgtttgtgcg caacaagcat ccggccggcc gtgaaacttc tccggacaag atcctgttgt
     2161 tcgttcatgg cgcgacctat ccggccgaga ccgcgttcga tctgccgatc gagggcgttt
     2221 ccatgatgga tctgatcgcg gcgcgcggct acgatgtcta tcttgtcgat gtcaggggtt
     2281 acggtcgctc gacacggccc gccgaaatga gccagccgcc ggcggcgaac aaaccgatcg
     2341 tgtcgaccaa ggtcgcggcg cacgacctcg gcatcgccgt cgattacatc ctcaggaaac
     2401 gcaaggtttc aaagatcaac gtcatgggct ggtcgtgggg gacctcgatc gccggatcgt
     2461 acaccagcga gcacaatgac aaggtcaacc gtctcgtgct ctacgcgccg caatggatcc
     2521 gcaacgaacc gccggcgcct gcggccgcca atgtgccgcc gcttggcgcc tatcgactgg
     2581 tctccaagga ctcggccaag gtgcgctggc tgaaggatgt ccccgccgac aagcaggcgg
     2641 atttgattcc gcccggcgta ttcgatgcct gggcgaacgc aacctgggcg accgatcccg
     2701 agtcatcgaa gcagaaccca cccatgctgc gtgcgccgaa cggcgttgtc gaggacagcg
     2761 tgaactactg gagcgcgggc aaggccctgt acgaccccgg caagattacc gtgccgaccc
     2821 ttctgcttca tgccgaatgg gatgcagacc ttccgagcta cctcgcgcag ggctacttcg
     2881 cgcagttgaa gaatacgccc tacaagcggc tgatcgaact gagcgaggga acgcatacgg
     2941 tgatgatgga aaagaaccgc atgcaatttt tccgcgaact gatgggattc ctcgacgagg
     3001 aaaaaccact ggcgttgaaa taaaccgcca aactagagcc ccgtttcgat agaatcgaaa
     3061 cggggctcta gattcttgat ttgacgcgct ttcttgacgc gaaccggtct ccaccccgga
     3121 tcaagtccgg ggcaggcttt cgcttgaaaa cgctctggac atcttcagat ggaatgccgg
     3181 ccggatgggc cggcattctg cgtttgagcc ggcaccgccc gcgccttatc ctccaatcgg
     3241 cggattgaaa tcgctgaaca gcgcccgcac cattcgtact gaaaagagga aggcctgcga
     3301 tcaagcaaaa tagctaaaag ggagaagcaa cgatgaaaac gctccagatc gtctgcgcag
     3361 tgctcctgct tgtgctcggt tgttcgcaag cgtccgcgga agattcgcga gagcttgtgt
     3421 cagcgttgaa gcagggaggt catgtcatcg tattccgtca tggcgccacc gacgacagcc
     3481 agaaagatat ttatccgctt aattttgacg acatgaaagc ccagcggcag ttgagcgaga
     3541 aggggcggga catggcccgc cagatcggga ccgcgatcaa gaagctcggc attccaatcg
     3601 gtgagatcta cacaagcaga ctcaatcggg cgatcgaaac cggcaagctg ctgtccgaca
     3661 aggaagtaaa acccctcgat gcattgacgg acagcggcgc aggaagcgcc tctgcgatgg
     3721 cgaaccccgc cggcggcaac gcaaaggcag gcctcgccct gcgggagctg gtaaatgccg
     3781 cacccaaggc cggcacgaac actttcatcg ttacacacaa gaccaatatc gcggatgcat
     3841 tcggaaaaga ggcgagcgat gtgcaggaag gcgaagcatt cgtctaccgg tccagtggat
     3901 cggctcccgc ggcatttgtc acccgcgtaa agccggcatt ctggatcgac caggccgcca
     3961 actgatccag ccgggtccag acgtaagatc ctgcgcgagc tgtcacaccc cggcaatggc
     4021 ggtgtcacat ggctgttgaa cgcccgtgat ttctgaatcc gaagaccgcg ccgattcgaa
     4081 tcggacagat gcggtcaaag ggatgtccat gcagaacggc gaatttgcgc agcgctttat
     4141 tgagatgctg cggccggaga atgtcgggaa cgcggcgcaa acggcgatca cctggctcaa
     4201 gccgccgccg gcgccgatag gcagcaagcc cctacgttta gccaattttc cggccacgct
     4261 tgggcggttg ggcgcgctcg aagtgcggct ggcgcaggaa aagcgtgacg tgaggcgcgc
     4321 ccaaaaactg cgttatcacg tgttctacaa ggatggcgcg gccattgccg acgccgcgac
     4381 gctgctggcg cgccgcgaca aggatgcgtt cgacaagatc tgcgaccatc tcatggtgat
     4441 cgatcacgca gccaaaccgt cgatcggcgg aaagcagccg gtggtcggca cctatcgcct
     4501 gctgcgccag gacgtggcgg aaagccacgg cggtttctac accgaggacg agttcgatat
     4561 agccggtctc gtcgagcgtc atgccggctt gcgtttcctc gaactcggac gatcctgcgt
     4621 gctgccgcgc tatcgcaaca agcgcaccgt cgaattgctg tggcacggga tctggagcta
     4681 tgtccgccag caccggctcg acgtgatgat cggctgcgcc agcttcgagg gcaccgaccc
     4741 ggatcggctt aaattgccac tgtcgttcct gcatcactac gcgcaagccc cggaacactg
     4801 gcgcgccaaa gcgcatcctt cgcgccgggt cgagatgaac cggatggcca aggaagcgat
     4861 caatccgaag gccgcccttc acgagctgcc gcccctgatc aagggatatc tgcggttagg
     4921 ggcttatatc ggcgatggtg ccgtgatcga tcatcagttc ggcaccaccg acgtgctgat
     4981 cgtgatgccg gtgtcggcga tcaatgcccg ctatatcgaa catttcggcg tcgacgcgtc
     5041 gcggcatgcg gcgtagcgct gcctttcacc tctccctttg ggagaggtcg aattgcgaag
     5101 caattcgggt gaggggttag ggcctaacga tggaccgtac cccccccaac cctctcccat
     5161 cgaagtcgga tatatccgac ttcgactata agaatgccca tctcgggtaa acccgagatg
     5221 gatgggagag ggagttcgct gccgtcggcg cgacaccgaa ccaatacctc agctcacgta
     5281 gatcggacgc tacagccgcc gcagcgccac ggtttcgacc atgtggtccg cgcccttttt
     5341 caggatcagc gtcgcacgcg gccgggtcgg caaaatattc tcttcgagat tggcaaggtt
     5401 ggtccgctcc cagatcgcca atgcggttgc
//
LOCUS       DASWGM010000803         6964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2607947,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000803 DASWGM010000000
VERSION     DASWGM010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2607947"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..308
                     /locus_tag="VGA15_22175"
     CDS             156..308
                     /locus_tag="VGA15_22175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590439.1"
                     /translation="MKVEFLSFLEGALELSILLNIFMAGLCGLLIYQDRKTAVPVSNG
                     TRSRMG"
     gene            368..619
                     /locus_tag="VGA15_22180"
     CDS             368..619
                     /locus_tag="VGA15_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590440.1"
                     /translation="MLIMLTVLVLVCSMSVTPDYLRNCDRNNAVQVMRLPEQIASPSM
                     CAMRGEAYIAGTTIGQQLAEDERVKVLCIKSGNLPAHMG"
     gene            complement(1511..2455)
                     /locus_tag="VGA15_22185"
     CDS             complement(1511..2455)
                     /locus_tag="VGA15_22185"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX9590441.1"
                     /translation="MSFASEIADIARVSRAASSARALARARDFIALTKPRVMSLAVFT
                     ALVGFVLAPGHPDQLAASIAMLCIAAGAGAAGALNMWYDADIDAMMARTALRPIPRGR
                     LSRGEALVLGLVLAASAVLVLAVATNVAAAALLASTIFFYVVVYTMWLKRRTPQNIVI
                     GGAAGATPPLIGWLAAGGPFALEPFLFVAIIFLWTPPHFWALALNRAGEYARAGIPML
                     PVVAGRVATKRQILIYCVLLVGAASLPWAIGAAGTVYAVASGICGAAMIALGVRLDRS
                     RYPDARAARRLFAFSILYLFVLFTALLADAALTAHWAT"
     gene            complement(2505..2840)
                     /gene="cyoD"
                     /locus_tag="VGA15_22190"
     CDS             complement(2505..2840)
                     /gene="cyoD"
                     /locus_tag="VGA15_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEX9590442.1"
                     /translation="MNVSATIRPAEPRGGLQSYVTGALLALALTLAPFALVMSGASGT
                     NAVAGIFGLAVIQIVVHMVFFLHLGRSDQRWNLTAFVFTVLIVGILVGGSYWVMYHLD
                     HSMMPMTME"
     gene            complement(2837..3490)
                     /gene="cyoC"
                     /locus_tag="VGA15_22195"
     CDS             complement(2837..3490)
                     /gene="cyoC"
                     /locus_tag="VGA15_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009364895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEX9590443.1"
                     /translation="MTSELLTVETVRPWIPPPSPQIIPAEQSEHRSGAVTTVLGFWVY
                     LMSDCLLFAALFATYSVLGREFAGGPTSKELFDLPYVLAETLCLLASSFTYGMVMVAL
                     KAGNRGRVLGFLLLTFVLGLGFIGLEVKEFVRMIGEGAGPDRSAFLSAFFTLVGTHGL
                     HVTGGLVWMAVMLVQASLRRFDAVLPTRLTCLSLFWHFLDIIWIGVFTVVYLAGAMS"
     gene            complement(3500..5464)
                     /gene="cyoB"
                     /locus_tag="VGA15_22200"
     CDS             complement(3500..5464)
                     /gene="cyoB"
                     /locus_tag="VGA15_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEX9590444.1"
                     /translation="MLGKLSLDALPLDQPVIMGAFGFMGLVAFSVLAALTYFGRWKWL
                     WTEWLTTVDHKKIGVMYIILALVMLLRGFSDAIMMRTQLALAHGGAAGYLPPEHYDQI
                     FSAHGAIMIFFMAMPFVIGMINIVVPLQIGARDVAFPFLNSLSFWLTVAGFGLINVSL
                     GIGEFARTGWLAYPPLSELGYSPGVGVDYYIWSLQISGVGTTLSGVNFIATILRNRAP
                     GMTLMRMPVFTWTALCSSIQIVAAFPILTICLALLSLDRYLGMHFFTNDLGGNQMMYV
                     NLIWAWGHPEVYILILPAFGIFSEVVATFSGKRLFGYVPMVVATMVIAVLSFLVWLHH
                     FFTMGAGGDVNGFFGIATMIIAIPTGVKVFNWLFTMYRGRIRFSVPILWTIGFMITFV
                     IGGMTGVLLAVPPADFVLHNSLFLVAHFHNTIIGGVVFGLLAGFNYWFPKAFGFRLNE
                     KLGRCAFWCWLVGFYLAFIPLYIVGLMGMTRRLNHYDNPDWHPFMIVAAVGAVVILCG
                     IVFQLVQLVVSIVQREKTRDLTGDPWNGRTLEWAIPSPPPAYNFAEIPIVTGRDAFWE
                     TKKSRTTRGVSRFQRIHMPRNTSAGLVIGGFALVLGFALVWHIWWMAAAGAVGIVATA
                     IAYSFKLNREYFLSALDVERSEKLLSPAAV"
     gene            complement(5470..6324)
                     /gene="cyoA"
                     /locus_tag="VGA15_22205"
     CDS             complement(5470..6324)
                     /gene="cyoA"
                     /locus_tag="VGA15_22205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEX9590445.1"
                     /translation="MAATLLLGGCNWGVLDPQGPIGEAERSLILESTLLMLIIVVPVI
                     VLVCAFAWRYRASNTKAKYMPEWDRSNAVAAVMVLAPVAIVSVLAVMAWQASHRLDPF
                     KAIESDAKPVAIDVVALDWKWLFIYPELRIATVNEIAFPTDVPVDFRITSASVMNSFF
                     IPQLGSQIYAMGGMQTKLSLMASRAGSYAGLSANYSGGGFSDMKFQAIASDQPSYERW
                     IAKVKAAPQNLSDGVYLALAKPSEKHPVEYFSSVAPDLFDQILHDTKSEPRAAAMLGA
                     ALCIANKE"
     gene            complement(6719..>6964)
                     /locus_tag="VGA15_22210"
     CDS             complement(6719..>6964)
                     /locus_tag="VGA15_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007801266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9590446.1"
                     /translation="RYFDKGNMAVVGKNYAVLERGWLRTSGFLTWLVWAFVHILSLPQ
                     LQNRLRVQHQWVWSYFTGQRSSRLIPELPRTPGVDTR"
BASE COUNT         1485 a         2138 c         2216 g         1125 t
ORIGIN      
        1 gtaccttgtc acacaagggc ccgcgcagca cctgcggcat acaatacagg gcgcctccgt
       61 aggaccgaac aggccgcctg aggcggatta atctaggcgg attgagaacg ggcgctgccg
      121 cgcatggaat cccgacggac aacaaggagg ttacgatgaa ggtcgagttt ttgtccttcc
      181 ttgagggggc gctggaacta tcgatcctgc tcaatatctt catggcaggg ctgtgcgggc
      241 tcttgatcta ccaggaccgg aaaacggcgg ttcccgtttc aaacgggacg cggtctcgca
      301 tgggatagcg gtctcgcatg ggatagaggc tggccgcaca gcttgggtgg cgtgtggaat
      361 cgcaggagtg ctgatcatgc tcactgtgtt ggttctcgtc tgctcgatgt ccgtcacccc
      421 ggactatttg cgcaattgtg accgcaacaa tgccgtccag gtcatgcggc tgcccgaaca
      481 gatagcctcc ccgtcgatgt gcgcgatgcg cggagaggcc tacattgccg ggacgacgat
      541 cggccagcaa ctcgccgagg acgagcgcgt caaagttctt tgcatcaaga gcgggaactt
      601 gccggctcac atgggatagc cttggcgcaa atgacagctg cgagtctcgc agcacaattg
      661 cactgacgat cgacgcccgc tcccgcccca tccgggccca tttggtatag gatccccgac
      721 cgtcggcgcg cggagagagg ccgcctagcc gcggctgcaa tcgtggaacg gacgcgcctc
      781 tatttcgatg aagggcgaat tcagggagga gcagcctgcc tcgaccggct cgagcggctg
      841 gcggatatcc cgtaaccacg cgctgcgcct ggtccgacat tcatcgctac gcccttctgg
      901 cgcgggccta tttcgcctcg gcgcaagacc gcctggatga ggcgagttcc ctcttgcagg
      961 acttgcggaa ggaatgcgag agcgcgggca gccgctacat ggcaccccgg gtatcgatgc
     1021 acttggcgat actgctgtgg cgtgcgcggc aggtcgcgaa tagcccagac gaatttgcct
     1081 ccgtcgcggc agtggccgcg gaaaacggta tctgccaggt catccttgat gagggaacga
     1141 agctggacgc gttgctgacg gagtttcacg aggcggcgag cggcaaaata ccgccacctc
     1201 gtgccctacg tgagcggctt cccgaccttc ggcgccgggc cgaggggccc gcggccggcg
     1261 catcaacgaa ttcagctggt gcggacgctc gcgggaagac aaaatcctga ggctgatcgc
     1321 cgaagggctt tccaataaga ccatggcgcg cgagctggcg atcgctcccg aaatggtcaa
     1381 gtcgcacgtg aagcgcatct tcacgacgac cggctcgagc tcgcgcgcga ccaggcggtg
     1441 tcgcgtgcgc agagtctcgg ctgctcagga cgcagccagc ttgaagttcc aacgggaacc
     1501 gcttcctgct tcacgtggcc cagtgggccg tcagtgccgc atctgccaac agcgccgtga
     1561 agagcacgaa cagataaagg atcgaaaagg caaacaggcg acgcgccgct cgcgcgtctg
     1621 gatagcggct gcgatccaat cggaccccga gcgcgatcat cgcagccccg cagatgcccg
     1681 aggcgacggc gtaaaccgtg cccgcggcgc cgatcgccca cggcagtgac gcggcgccaa
     1741 ccagaaggac gcagtagatc agaatctgcc gcttggtggc gaccctgccc gccacgaccg
     1801 gcagcatcgg gatgccggca cgagcgtatt cgcccgcccg attgagcgcc aacgcccaga
     1861 agtgaggcgg cgtccacagg aagatgatgg cgacgaaaag aaagggctcg agcgcgaagg
     1921 gaccgcccgc cgcgagccag ccaatcagcg gcggagttgc gccggcggcg ccgccgatga
     1981 cgatgttttg cggggtgcgg cgcttgagcc acatcgtgta aacgacaacg taaaagaaga
     2041 tagtcgacgc gagcagcgcc gcggccgcaa cattagtcgc aaccgcgagg acaaggacgg
     2101 cggacgccgc caggacgagg ccaagcacca gcgcttcgcc ccgcgacagg cggccacgcg
     2161 ggatcgggcg caaggcagtg cgggccatca tcgcgtcgat atcggcgtcg taccacatgt
     2221 tgagcgcgcc ggcggctcct gccccggccg caatgcacag catggcaatc gatgcggcaa
     2281 gctgatcggg atgaccgggc gcgagcacga acccaaccag cgccgtgaac acggccagag
     2341 acatcacgcg tggctttgtc agcgcgatga agtctcgagc cctcgcgagg gcccgcgcgg
     2401 acgaagcggc tcggctgacg cgggcgatgt cggcaatttc gctggcaaaa gacatggacc
     2461 gtcccttcct caggatttga taagttttct ctctccgagt tcatttattc catggtcatc
     2521 ggcatcatgc tgtgatcgag gtgatacatg acccagtacg agcctccgac caggatcccg
     2581 acaatcagga cagtgaagac gaacgccgtg agattccagc gttgatccga gcgacccagg
     2641 tggagaaaga acaccatgtg cacgacgatc tggatcacgg ctaagccgaa aatgcctgcg
     2701 acggcgtttg tgcccgacgc tccgctcatg accaaggcaa agggagcgag cgtcagcgcg
     2761 agggcgagaa gcgcgcccgt gacatacgat tgcaggcccc cgcgcggttc ggcggggcgg
     2821 atcgttgccg agacgttcat gacatggctc ctgccaggta gacgacggtg aacacgccga
     2881 tccagatgat gtcgagaaag tgccagaaca ggctcaaaca ggtgagccgg gttgggagaa
     2941 cggcgtcgaa cctgcgcagc gacgcctgca ccagcatgac cgccatccag accaatccgc
     3001 cggtcacgtg caggccgtgc gtgccgacca gggtgaagaa ggcggacagg aacgcgctgc
     3061 ggtcgggccc cgccccttcc ccgatcatgc ggacgaattc cttcacttcc aggccgatga
     3121 aaccgagccc gagcacgaac gtcagcagca ggaacccgag tacgcgcccg cggttacccg
     3181 ccttgagcgc caccatgacc atgccgtagg tgaagctgct cgccagcagg cacagtgttt
     3241 cagcgagcac gtacggaaga tcgaacagct cctttgaggt cgggcctcca gcgaactcgc
     3301 ggcccagcac cgaataggtg gcgaacagcg ccgcgaacag aagacagtcg ctcatcaggt
     3361 agacccaaaa gccgagcacc gtggtgacgg caccactgcg atgctcgctt tgctctgccg
     3421 gtatgatttg cggcgacggc ggcggaatcc acggtcggac ggtttcgacg gtcagaagtt
     3481 cgcttgtcat ggcgttcacc tacactgccg ccggggagag gagcttctcg ctgcgctcca
     3541 cgtcgagcgc ggagaggaag tactcgcggt tgagcttgaa cgaataggcg atggcggtgg
     3601 cgacgatccc cacggcgccg gcggccgcca tccaccagat gtgccagacc agggcaaaac
     3661 cgagcaccaa ggcaaacccg ccgatcacca ggcccgcgct ggtgttgcgc ggcatgtgaa
     3721 ttctctggaa gcggctcacc ccgcgcgtgg tgcggctctt cttcgtctcc cagaatgcgt
     3781 cgcggccggt gacgatgggg atttccgcaa aattgtaggc cggcggcggc gagggaatcg
     3841 cccattccaa ggtgcgcccg ttccacggat cgccggtcag gtcgcgggtc ttctcccgct
     3901 gcacgatgct gaccacgagc tgcaccagct gaaagacgat cccgcacagg atcaccaccg
     3961 cgcccaccgc cgccacgatc atgaacgggt gccagtcggg attgtcgtaa tgattgaggc
     4021 ggcgggtcat gcccatgagg ccgacgatgt agagcgggat gaacgccagg tagaagccga
     4081 cgagccagca ccagaaggcg caccggccga gcttttcgtt gagccggaag ccgaacgcct
     4141 tcgggaacca atagttgaag cccgccagca acccgaacac gaccccgccg atgatggtgt
     4201 tgtggaaatg cgcgacgaga aacaggctgt tgtgcagcac gaagtccgcc ggcgggaccg
     4261 ccagcaacac gccggtcatg ccgccgatca cgaaggtgat catgaatccg atcgtccaca
     4321 ggatcggcac ggagaagcga atgcggccgc gatacatggt gaacagccag ttgaaaacct
     4381 tcacgccggt cgggatggcg atgatcatcg tggcgatgcc gaagaagccg ttcacgtcgc
     4441 cgcccgcccc catggtgaag aagtgatgga gccaaaccag gaaggaaaga accgcaatga
     4501 ccatggtggc caccaccatg gggacgtagc cgaacaggcg cttgccggag aacgtcgcaa
     4561 ccacctcgga aaaaatccca aaggcgggaa ggatcaggat gtacacctcc gggtgccccc
     4621 aggcccagat caggttgacg tacatcatct gattgccacc gaggtcattc gtgaagaagt
     4681 gcatgccgag ataccggtcg aggctgagca gcgcgaggca gatcgtcagg atcgggaacg
     4741 ccgcaacgat ctgaatgctg ctgcacagcg ccgtccaagt gaaaacgggc atgcgcatca
     4801 gggtcatgcc gggggcgcga ttgcgcagaa tggttgcgat gaagttcacg cccgaaagcg
     4861 tggttcccac ccccgagatc tgcaggctcc agatgtaata gtcgacgccg acgccagggc
     4921 tgtaaccgag ttccgagagc ggcgggtagg cgagccatcc cgtgcgcgcg aactcgccaa
     4981 tccccaatga aacgttgatc agtccgaacc ccgccacggt gagccagaag ctgagtgaat
     5041 tgaggaacgg aaatgctacg tcgcgtgcgc cgatctgcag gggcaccacg atgtttatca
     5101 tcccgatcac gaacggcatc gccatgaaga agatcatgat cgcgccgtgt gcggagaaga
     5161 tctggtcgta gtgctcgggc ggcaggtagc cggcggcacc gccgtgcgcc agggccagct
     5221 gcgtccgcat catgatggcg tcggaaaacc cacgcagcag catcaccagc gccaggatga
     5281 tgtacatcac gccgatcttc ttatggtcga cggtggtcag ccactcggtc cacagccact
     5341 tccatcggcc gaaatacgtg agagcggcga ggactgagaa tgcgaccagg cccatgaacc
     5401 cgaacgcccc cattatgacc ggctgatcca gagggagcgc gtcgagggac aactttccga
     5461 gcatggccgc tactccttgt tggcgatgca gagggcggca ccgagcatgg cggcggccct
     5521 cggctcgctc ttggtgtcgt gcaggatttg gtcgaacagg tcgggcgcga ccgaggagaa
     5581 gtattcgacc gggtgtttct cgctcggctt tgccagcgcc aggtagacgc cgtcgctcag
     5641 gttttgcggc gccgccttca ccttcgcgat ccagcgctca tacgacggct ggtccgaggc
     5701 gatcgcctgg aacttcatgt cggagaagcc gccgccgctg tagttcgcgg acaggccggc
     5761 atagctgccg gcccggcttg ccatcagact gagcttcgtc tgcatgccgc ccatggcata
     5821 gatctggctg ccgagctgag ggatgaagaa cgagttcatc accgaggcgg aggtgatcct
     5881 gaagtcgacc ggtacgtcgg tggggaacgc aatctcgttc accgtggcga tgcgcagttc
     5941 cgggtagatg aacagccatt tccaatcgag cgccacaacg tcgatggcga ccggcttcgc
     6001 gtcagactcg atcgccttga aaggatcgag ccggtgcgac gcctgccatg ccatgacggc
     6061 caggaccgaa acgatcgcca ccggagcaag caccatcacg gcggcgaccg cgtttgagcg
     6121 atcccactcc ggcatgtact tggccttggt gttcgaggcc cgatatcgcc aagcgaacgc
     6181 gcaaaccagc acgatcaccg ggaccacgat gatcagcatc aagagggtcg attcgaggat
     6241 aagcgagcgt tcggcctcgc caatcggccc ctgagggtcg agaacgcccc agttgcagcc
     6301 gccaagaaga agggtcgccg ccaagggcag cagcctcggt attcgattgt atctgacgct
     6361 tcgcatcggg aggccttttc atgccggtgc cggcgtagaa tgcacgtctc gtcgagcgaa
     6421 aaacgcattc gcagccggtc ttacgtcttc ccgatagcta caccgcgcgt gccgaagagc
     6481 gtgagtgaat gctcccaaat cctagcaaat ttttcataag cagcgacgcg atcaataaaa
     6541 caaagcgtgc gcgatggtcc ggcgacttta gacactactc gccatgcgcc gccaaagcgg
     6601 ggcatccagt atccgctatc cgccaccgtc gcagataagt cgcaaagggc aacgtttagt
     6661 agatcgtccg cttttgcggg cgatgacatt tgattggtcg atgccgataa cgccggaatt
     6721 atctcgtgtc cacgcccggc gttcgcggca actccgggat caatcgtgag ctgcgctggc
     6781 cggtgaaata cgaccaaacc cactgatgct gcacgcgcag gcggttctgc agctgcggca
     6841 gggagaggat atgaacgaac gcccacacca gccacgtcag gaagccgctt gtgcgcagcc
     6901 agccccgctc cagcaccgcg taattcttgc cgacgacggc catgttgccc ttgtcgaaat
     6961 agcg
//
LOCUS       DASWGM010000804         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_365500,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000804 DASWGM010000000
VERSION     DASWGM010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365500"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VGA15_22215"
     CDS             <1..283
                     /locus_tag="VGA15_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX9590447.1"
                     /translation="DKTVLFMANHGISTVGETVADAYDRLYYIERAAQVQVYAMWTGQ
                     PLKQLPAAVVEKTKRDIGGAELYEGPTPAQRHFDALKRILDRKEPDYAS"
     gene            complement(391..1044)
                     /locus_tag="VGA15_22220"
     CDS             complement(391..1044)
                     /locus_tag="VGA15_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9590448.1"
                     /translation="MARHPKAPRTLTHDQIWTALDRLAERAGLSPSGLAKRSGLDPTT
                     FNKSKRVTPDGRERWPSTESVSKALAATSSSIDTFVQLIGDSARTVQSVPLLGFAQAG
                     TGGYFDDGGLPAGKGWDEVGLPSVNDEHGYALEIAGDSMKPAYRDGDVIVVSPAMPIR
                     RGDRVVVKTRDGEVMVKELKRRTARTLELQSLNPNHVDHTLAAADVEWIARIVWVSQ"
     gene            1269..1613
                     /locus_tag="VGA15_22225"
     CDS             1269..1613
                     /locus_tag="VGA15_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEX9590449.1"
                     /translation="MRMIYKICSASAWREAERQGVYRGSADDFRDGFIHFSLPSQVAE
                     TARKHFFGQTGLFLIAVDADRLGDALRWEPSRNEELFPHLYGELDLGAVVAVRDLHAR
                     SDGYHDIPELAP"
     gene            1610..2704
                     /locus_tag="VGA15_22230"
     CDS             1610..2704
                     /locus_tag="VGA15_22230"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX9590450.1"
                     /translation="MIRAFDALSLPLLRWLDPEDAHRLAIQGLRLLPPVRPRADDPKL
                     AVRAFGLNFPNPVGMAAGFDKSAEVSDALLRLGFGFVEIGTVTPKPQVGNPRPRLFRL
                     ERDEAVINRMGFNNDGGDAVLRRLAGRAHLGGIVGVNVGANKDSADRVADYVKLIEIF
                     APVATYFTVNVSSPNTPGLRNLQQSAALDDLLAKVIDARERVRQSAGDSPVLLKVAPD
                     LSLTELDDVVHIARSRRIDGMIVGNTTLARPSTLRQQTRAKEQGGLSGRPLFRLSTRM
                     VAETYVRAEGAFPLVGVGGIDSGGAALTKIRAGASLIQLYSSLIYKGLHLVEDIKNDL
                     ASTLLRTGRDSLSEIVGADAATITAEDWPV"
     gene            complement(2744..>3370)
                     /locus_tag="VGA15_22235"
     CDS             complement(2744..>3370)
                     /locus_tag="VGA15_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9590451.1"
                     /translation="ASSLPALKRLTIARDAIDLILITHFHGDHFGGLPFLLLDAQFTR
                     RTRSLVIAGPEGIETRLTQVMEALFEHSSKTKQRFDLSVIALGPGKTRAFGAVKVTPF
                     AVVHGLSGGPFLAYRVEAEGRVIAYSADTEWTETLIPAAHDADLFIAEAYYFDKVVKN
                     HLSLKTLGAHLAEIKPKRLILTHMSDDMLGRLDTLDYTAASDGLIVEL"
BASE COUNT          602 a         1060 c         1079 g          629 t
ORIGIN      
        1 cgacaagacg gtgttgttca tggccaatca cggcatcagc acggtcggcg agaccgtcgc
       61 cgatgcctat gatcggcttt actacatcga gcgcgcggca caggttcagg tctacgcgat
      121 gtggaccggg cagccgctca agcagctccc tgccgccgtg gtcgagaaga ccaagcggga
      181 tatcggcggg gccgagctct acgagggccc aaccccggca cagcggcact tcgatgcact
      241 aaagcgcatc ctcgaccgca aggagcccga ctacgcttcg taggcggcgg acgccttcaa
      301 ttcgacaccg gcgcttaagc tcacctcgtc atgcgcgggc agaagcgcga agacgcgctt
      361 cgcgcttttg cccggcaatg acgatttcac ctactggctc acccacacga tccgtgcgat
      421 ccactcgacg tcggcggccg ccagggtgtg gtcgacatgg ttcggattga gcgattgcaa
      481 ttccagcgtc ctcgcggtcc ggcgtttcag ttccttgacc atcacctcgc catcccgggt
      541 tttcaccacc acgcggtcac cgcggcggat cggcatagca ggcgacacca cgatgacgtc
      601 accgtcgcga taggcgggct tcatcgaatc gccggcgatt tccagtgcat agccgtgctc
      661 gtcgttgacg gagggcaggc ccacctcatc ccagcccttg ccggccggta atccgccatc
      721 gtcgaaatag ccgccggttc ccgcctgcgc gaagccgagc agcggcaccg actgcacggt
      781 tcgcgcgctg tcgccgatca gctgcacgaa agtgtcgatg gaactgctgg tggcggcgag
      841 cgccttggat accgattcgg ttgacggcca ccgctcgcgc ccgtcaggcg tgacgcgttt
      901 ggatttgttg aatgtggtgg gatcgaggcc ggaacgtttg gcgagtccgg aaggcgacag
      961 tccggcccgc tccgccagcc gatccagcgc ggtccagatc tggtcgtggg tcagcgtgcg
     1021 cggcgctttt ggatgtctgg ccatgggtca ctttgtcaaa agctaggaat tattacctca
     1081 atcgaccggc tttcgcaatt cgggacaaga gactgcgaag cgacttacaa gcgcccgaat
     1141 gcgacccttg aagtgcgccg gtgccgtcga tacggtcgca gccaggtccg gaaattccag
     1201 ggaaatccca aggaaaacct caagggaatc ccgaggggac tcaaacgacc aggcaggact
     1261 ccgggcacgt gcgcatgatc tacaaaatct gttccgcttc ggcctggcgc gaggccgagc
     1321 ggcaaggcgt gtaccgcggc agcgccgacg atttccgtga cggcttcatt catttttctc
     1381 ttccctcgca ggtggcggag acggcccgga aacatttctt cggccagacc ggactgttcc
     1441 tgatcgcggt cgacgccgac aggctgggcg acgcgctgcg gtgggagccg tcgcgcaatg
     1501 aggagctgtt cccacatctc tacggcgaac ttgatctcgg cgcggtcgtc gctgtgcgcg
     1561 atctgcacgc gcgatccgat ggatatcacg acatcccgga gcttgcgccg tgatccgcgc
     1621 cttcgatgcc ctgtcattgc cgctgttgcg ctggctcgac ccggaagacg cgcaccgtct
     1681 ggcgatccag ggcttgcggc tgctgccacc ggtacggccg cgcgccgacg atccaaaact
     1741 ggcggtgcgg gcctttggcc tgaactttcc caatccggtc ggcatggccg caggcttcga
     1801 caagagtgcg gaggtttcgg acgcgctgtt gcggctgggt ttcggcttcg tcgaaatcgg
     1861 gacggtgacg ccgaagccgc aagtaggcaa cccgcggccg cggcttttcc ggctggagcg
     1921 cgacgaggcg gtgatcaacc gcatgggctt caacaatgac ggcggcgatg cggtgctgcg
     1981 gcggctggcc ggccgcgcgc atctcggcgg tattgtcggc gtcaatgtcg gggccaacaa
     2041 ggatagcgcc gaccgcgtcg ccgattatgt gaaactgatc gagatcttcg cgccggtggc
     2101 gacctatttc accgtcaacg tgtcgtcgcc gaataccccg gggctgcgca atctgcagca
     2161 gtcggcagca ctcgacgatc tcctggcaaa ggtgatcgat gcgcgcgagc gggtgcggca
     2221 gagcgccggc gattcgccgg tgctgctcaa ggtcgcgccg gatctgagcc tgaccgaact
     2281 cgacgatgtg gttcacattg cgcgctcgcg ccggatcgac ggcatgatcg tcggcaatac
     2341 cacgctggca cggccctcga ccttgcgcca gcagacccgc gcaaaagagc aggggggatt
     2401 gtcggggagg ccgctgttcc ggctgtcgac acggatggtg gcagaaacct atgtgcgcgc
     2461 cgagggcgca tttccgcttg tcggtgtcgg cggcatcgac agcggcggcg ccgcgctgac
     2521 caagatccgt gccggcgcca gcctgatcca gctctattcc tcgctgatct acaagggcct
     2581 tcacctggtg gaggatatca agaacgatct tgcctcgacg ctattgcgca ccgggcgcga
     2641 ctcgctgtcc gaaatcgtcg gcgcggatgc ggcgacgatc accgccgagg attggccggt
     2701 ttagggcgta atgcgcatcg tggtggatga gtccatctcg tgcttacagc tcgacgatca
     2761 ggccgtcgct cgccgcggtg tagtccagcg tatcgagccg ccccagcatg tcgtcgctca
     2821 tatgggttaa tatgagccgc ttcggtttga tctcggccag atgcgccccc agcgttttca
     2881 ggctgaggtg atttttgacg accttgtcga agtaataggc ctcggcgatg aacaggtcgg
     2941 cgtcgtgcgc cgccgggatc agcgtttccg tccattcggt gtccgcgctg taggcgatga
     3001 cgcggccttc ggcctcgacc cggtaggcca gaaacggacc gccggacaag ccgtgcacca
     3061 ccgcaaacgg cgttactttc accgcgccga atgcccgcgt tttcccaggc ccgagcgcaa
     3121 taaccgacag gtcaaacctc tgttttgttt tcgaggaatg ctcgaacaac gcttccatca
     3181 cttgcgtcag ccgcgtctcg attccttcgg gtcccgcgat caccagcgaa cgcgtccgac
     3241 gggtgaattg cgcatcgagc agcagaaacg gcaggccccc aaagtgatcg ccgtggaaat
     3301 gcgtgatcaa aatgaggtcg atagcatcgc gcgcgatggt gagccgcttc agcgccggca
     3361 gcgaggatgc
//
LOCUS       DASWGM010000805         1864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1457298,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000805 DASWGM010000000
VERSION     DASWGM010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457298"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..>1864
                     /locus_tag="VGA15_22240"
     CDS             2..>1864
                     /locus_tag="VGA15_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9590452.1"
                     /translation="MIWNGAQVLGSAWDDVRQVRESDKILALLESETGRLQNLIHRYI
                     NQPSPELFAEILLLREAVLGTLTTRASTDPMLSGSVEELERVTERFLNGFGELRGVQA
                     TISKTYEEQVLTPAREMAGLYSIIEGATGHRDALIWPSLGKSREAFTAMLVAANAYYL
                     SLATASAEEARRSTETIEKTIPVMSDLADNDLQRMALRRLNARTVALREGLAKLSEEL
                     ASRTELLRNTIDASQADAIGAIDRLSVKMRQREQKAQETFDRTLADISRRVMSIAVIF
                     LGIILTAGVLIALSIRLPLHQIMSAMRAITTGDYDRRVQGTDATDEVGAMARAVEVFR
                     ENAIAKRKTEDELRNSKEKAESALLELNAAQQNLIDAERLAALGGLVAGVAHEVNNPI
                     GISLTVASSFARRAEMFEAELRSDGRLRRSQLEEFVRTSRDAAQQLVANLHRAGELIQ
                     SFKQVAVDRSHAERRQFGLSEATDQIIASLRPVLKKAAIALSVDVPEGLVIDGYPGSY
                     GQILTNLFLNAANHAFPDGRSGTISISAKPRGSDDVEIIFADNGAGMTPDVQRQAFDP
                     FFTTRRNEGGTGLGLHIVYNLVTQQLGGRMMLDSRLGQGTTFRIIMPRAAKGGST"
BASE COUNT          358 a          578 c          604 g          324 t
ORIGIN      
        1 catgatctgg aacggcgcgc aggtgctcgg ttcggcctgg gacgacgtcc ggcaagtgcg
       61 cgagtcggac aagatcctgg cgttgctgga aagcgaaacc ggccgcctgc agaatctgat
      121 ccatcgttac atcaatcaac cgagccctga actgttcgcg gagatattgc tgttgcgcga
      181 ggcagttctc ggtacgctta ccacccgcgc gtcgaccgat ccgatgctgt cgggatcggt
      241 cgaggagctt gagcgcgtca ccgagcgttt cctgaacggc ttcggcgaat tgcgcggcgt
      301 ccaggccact atttctaaaa cctacgaaga gcaggtcctg acccccgcca gggaaatggc
      361 cgggctttat tcgatcatcg aaggcgcgac agggcaccgc gatgcgttga tctggccgtc
      421 gcttggaaag tcccgcgagg cgtttaccgc catgttggtc gccgccaacg cctattatct
      481 ctcgctggcg acggcatcgg ccgaggaagc ccgtcgcagc accgagacga tcgagaagac
      541 cattccggtg atgtccgacc tcgccgacaa cgacctgcag cgcatggcct tgcggcgatt
      601 gaatgcgcgg acggtggcgc tgcgggaagg cctggcgaaa ttatcggaag agctcgcgag
      661 ccggaccgag ctgttgcgca ataccatcga cgccagccag gccgacgcca tcggcgccat
      721 cgatcgattg tcggtcaaga tgcggcagcg cgagcagaaa gcgcaggaga cattcgatcg
      781 gacgctggcc gatatatccc gcagggtgat gtccatcgcc gtgatttttc tcggcatcat
      841 cctcaccgcg ggcgtcctga tcgcgctgag catccggctg cccctgcatc agatcatgtc
      901 ggcgatgcgc gcgatcacca cgggcgatta cgaccggcgc gtgcagggca cggatgccac
      961 ggacgaggtc ggcgcgatgg cgcgcgcggt ggaggtattc cgcgaaaacg cgatcgccaa
     1021 gcgcaagacc gaggacgagt tgcgcaattc caaggagaag gccgagagcg ccctgctcga
     1081 actgaacgcc gcccagcaaa acctgatcga cgcggaacgt ctggcggcgc ttggcggtct
     1141 ggtcgccggt gtcgcccatg aagtgaacaa cccgatcggc atcagcctga cggtggcgtc
     1201 cagtttcgcg cgccgcgcgg aaatgttcga ggccgagctg agatccgacg gtcggttgcg
     1261 ccggtctcaa ctggaggaat tcgtccggac ctcgcgcgac gcagcccagc aactggtggc
     1321 gaacctgcat cgcgccgggg aactgatcca gtccttcaag caggtggcgg tggaccgctc
     1381 gcatgcggaa cgccggcagt tcggcctgag cgaggcgacc gaccagatca tcgccagcct
     1441 gcggccggtc ctgaagaagg cggcgatcgc cctgtcggtc gacgtgcccg agggcctggt
     1501 gatcgacggc tatccgggct cttatgggca gatcctgacc aatctcttcc tcaacgcggc
     1561 aaatcatgca tttcccgacg gacgctccgg gacgatctcg atttcggcca agccccgcgg
     1621 cagtgacgac gttgagatca tttttgccga caatggggcc gggatgacgc cggacgtgca
     1681 gcggcaggcg ttcgacccgt tctttaccac gcgccgcaac gagggcggca ccgggctcgg
     1741 cttgcacatt gtctataatc ttgtcactca gcagcttggc ggccggatga tgctggattc
     1801 aaggctggga caaggcacca catttcgcat tatcatgccg cgggccgcca aaggcggctc
     1861 caca
//
LOCUS       DASWGM010000806         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1889781,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000806 DASWGM010000000
VERSION     DASWGM010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889781"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..938
                     /locus_tag="VGA15_22245"
     CDS             45..938
                     /locus_tag="VGA15_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEX9590453.1"
                     /translation="MAGVLSSLRYLLADIIGDDDEAPPFLPDGLPEGAAPIVSEGIHL
                     LIDYQGPSYAGLYVDRLRRFVGRRGVDDVMFGEIARLMAMRMSYEDPIRIAQSKLAET
                     EIASGASNVQSVDDVRKFRLDELIGALPAIVAEPVLDALDWVGWLHMLVSIRFSNASR
                     WGIRRLRIEAGLRRWRLFSVRYAKERVWVERWLHMIDRSLTKQPAAASAIARTATMVQ
                     GYGDAYRQALADWHAIIDGLVKPTFDEDLPLADLAAAVAEARAAVTPDPRQAALKRTI
                     AEIRARALAEARQPVVEASGE"
     gene            complement(988..2388)
                     /locus_tag="VGA15_22250"
     CDS             complement(988..2388)
                     /locus_tag="VGA15_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX9590454.1"
                     /translation="MSAELPAPDAETPPAQSQVVRSNRRQVLLFAALTLILTVATVWG
                     GRTWVRNSETLIFAVGDANSLDARFAAKLAAVLKNSSSRLRLKIVANADSAKALAQFD
                     RRQADLAVLRTDAKVPPRARALAILEHDVLLLISPGGKKIKSLAELKKKKIAVLADAD
                     NSVAFVRTILEISDNADGGPRVQMAPPNSTIEKLFASGGYGAAIAIAHASKIIKDKSY
                     EQSARHGGFTLNAIDEAKALARKNPGISEETLTAGMLSSAPAIPDDDLDTIGLQWLLV
                     AQSRMSSTTAGDLARMIYENKAELALADGFASRIEPADTDKDAFIVAHQGAAEYINDD
                     TKSFMDRYSDLLYLGAAALSVIGSIFAGIYTKFTRVAPEKAGELATAILDIGERIEHA
                     DSLDQLEALQDELEAILRGAVIGLRDGTISSDGLDTFRLGYEFVRDEIGMRRDHLKRN
                     AGHDGNVVVVKSAQSA"
     gene            2776..3132
                     /locus_tag="VGA15_22255"
     CDS             2776..3132
                     /locus_tag="VGA15_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEX9590455.1"
                     /translation="MLVLTFKHIIADFVLQTSWMAIGKDQKTGWALPLLAHCLVHLAV
                     AMTLILIIAPKFWFVALIDFAIHITVDRAKGFCASRFGVTQEDHHPWFWTLIGVDQAL
                     HHLTGFGLSILMAANG"
     gene            complement(3113..3616)
                     /locus_tag="VGA15_22260"
     CDS             complement(3113..3616)
                     /locus_tag="VGA15_22260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9590456.1"
                     /translation="MPLAFTISDLRQRPEFFDSVADRIWQAWWKADGHPLEYISGRLR
                     ENLSAAAIPFALVAHDGEVFLGTTSVIASDLAERPQLTPWVAAVWVDPQARQRGVGAA
                     LVDRATRACFALGVRRAYLCARPLRSGYYERLGWTPIERGVGPHHLSVLIRDAGAEAG
                     NISRSPP"
     gene            3774..>4200
                     /locus_tag="VGA15_22265"
     CDS             3774..>4200
                     /locus_tag="VGA15_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9590457.1"
                     /translation="MTERLLTKKTVDVLGSRMAYHERGEGTPVLFLHGNPTSSYLWRD
                     VIPELEGCGRLIAPDLIGMGDSAKLPNPDTDTYRFTTHRKYLAGFIDAVIGPAQPIVF
                     VVHDWGSALGFDWANHHRDRVRGIAYMEGIVRPVAGWEEW"
BASE COUNT          760 a         1271 c         1308 g          861 t
ORIGIN      
        1 cacgccgcgg ccgctgcacc gaatcccgct taaaggccgc tgcagtggca ggcgtcctgt
       61 cttcgctgcg ttatttgctc gccgatatta tcggcgacga cgacgaggcg ccgccgtttt
      121 tgcccgacgg gctgcccgag ggtgccgccc ccatcgtcag cgaaggcatt catctcttga
      181 tcgactacca gggccccagc tatgcgggcc tttatgtgga ccggttgcgg cggttcgtcg
      241 gccgccgcgg cgtcgatgac gtcatgttcg gcgaaatcgc gcggctgatg gcgatgcgca
      301 tgagctatga ggatccgatc cggatcgcgc agtcaaaact cgccgaaacc gaaattgcct
      361 ccggcgcttc gaatgttcag tccgtcgacg atgtcaggaa attccggctc gatgaactga
      421 tcggggcgct tcctgccatt gtcgccgaac cggtactcga tgccctggac tgggtggggt
      481 ggctacatat gctggtctcg atccgcttca gcaacgcgag ccggtggggc attcgccgtt
      541 tgaggatcga ggcgggcttg cggcgatggc ggttgttctc ggtgcgctat gccaaggagc
      601 gggtctgggt cgaacgctgg ctgcacatga tcgaccgcag cctgaccaag cagccagcgg
      661 cagcgtccgc gatcgcgcgg accgcgacca tggtgcaggg ctacggcgac gcctatcgcc
      721 aggcgctcgc cgactggcat gcgatcatcg atggcctcgt caagccgacc ttcgacgagg
      781 atctgccgct agccgatctt gccgccgccg tggcggaggc ccgcgccgcg gtcacgcccg
      841 atccgcggca ggccgcgctc aagcgcacca tcgcggagat cagggcgcgg gcgctcgccg
      901 aagcccggca accggtcgta gaggcgagtg gcgaatagtg agtagcgaat ggaaggaaaa
      961 aactattcgc tattcgccgt tcgccttcta cgcgctttgc gctgacttga cgaccaccac
     1021 attgccgtca tggccggcgt tacgcttcag gtgatcgcgg cgcatgccga tctcgtcgcg
     1081 cacgaattcg tagccgagcc tgaacgtatc cagcccgtcg cttgagatgg tgccgtcgcg
     1141 caatccgatc accgcgccgc gaagaatggc ttcgagttcg tcctgcaatg cctcgagctg
     1201 gtccagcgaa tcggcgtgtt ctatcctctc gccgatgtcg aggatagcgg ttgcaagttc
     1261 gccggccttc tccggcgcga cgcgggtgaa cttggtgtag atcccggcaa agatcgagcc
     1321 gatcacgctg agcgcggctg cgccgagata cagcaggtcg ctgtaacggt ccatgaacga
     1381 cttggtgtcg tcgttgatgt attcggcggc gccctggtgc gccacgatga aggcgtcctt
     1441 gtcggtgtcg gccggctcga tcctggaggc gaagccatcg gcgagcgcga gttcggcctt
     1501 gttttcgtag atcatgcggg cgagatctcc cgctgtcgtc gatgacatcc tggactgcgc
     1561 gaccagcagc cattgcagcc cgatggtatc gagatcgtca tcgggaatcg cgggtgccga
     1621 cgacagcatg cctgccgtca gcgtttcctc cgaaatgccg gggttttttc tggccagcgc
     1681 tttcgcctcg tcgatcgcgt tcagggtgaa cccgccgtgc ctggcagact gttcatagct
     1741 cttgtctttg atgatcttcg aggcatgcgc aatggcgatc gccgcgccgt agccgccgga
     1801 cgcgaacagc ttttcgattg tcgaattcgg cggcgccatc tgcacccgtg gaccgccatc
     1861 cgcgttgtcg gaaatctcga ggatggtgcg cacgaacgcg acgctgttgt cggcatcggc
     1921 caaaacggcg atcttctttt tcttcagctc ggcaagcgac ttgatctttt tgccgccagg
     1981 gctgatcagc agcagcacgt cgtgctccag gatggcaagc gcgcgggcgc gtggcgggac
     2041 ctttgcatcg gtgcgcagca ccgcaagatc ggcctgcctg cgatcgaact gcgcgagcgc
     2101 cttggcgctg tcggcgttgg ccacgatctt gaggcgcagc cgcgaagagc tgttcttgag
     2161 caccgcggca agcttggcgg caaaacgggc gtcaaggctg ttggcgtctc cgacggcgaa
     2221 gatcagcgtt tcggaattcc tcacccaggt gcgaccgccc caaactgtcg cgacagtgag
     2281 gatcaatgtc agtgcggcaa acaggagcac ctgcctgcgg ttgcttctga caacctgcga
     2341 ctgcgcaggc ggcgtttccg catctggcgc cgggagttca gcgctcatgg ctcatatccg
     2401 atcgcccgaa cgagatcgta aatatctaaa aagaacgaca attttccggt ttaaggatga
     2461 tatccgtggc ggcgccgaat gcaaatcggg ctgccgggtt aacgttaccg gtggacccgc
     2521 ttattgatgt tgctgtgcat tatcactgtt aggtcacacc tagcgttttt tcgattcggc
     2581 gcaggtgcat tccagctcgg gaggtgtcat aaataagtaa tatcgcgcct gaattgatcg
     2641 ggattgaaga gaagttgcgc tgacagccta agttacagtt cttaatgtca ttccagtcag
     2701 tcccagcaaa gggcgtcagc cttgttgttt ccccccatgt cttcgtcggt tccggtaggt
     2761 gcgctggtag ggtggatgct ggtcctcacg ttcaaacaca tcatcgcgga tttcgtgctg
     2821 caaacctcct ggatggcgat cggcaaggac cagaagaccg gctgggcgtt gccgctgctg
     2881 gcccactgcc tggtgcatct tgcggttgca atgacgctga tcctgatcat cgcgccaaaa
     2941 ttctggtttg tcgctctgat cgatttcgcc attcacatta cggtcgaccg cgccaaggga
     3001 ttttgcgcat cgaggtttgg cgtgacgcag gaggatcatc atccatggtt ctggacgctg
     3061 atcggggtag accaggcatt gcatcacctg accggattcg gcctgtcgat tctcatggcg
     3121 gcgaacggct gatattgccg gcttccgcgc ctgcatcccg gatcaacacg ctcagatggt
     3181 gtggcccgac accgcgttcg atcggggtcc agcccagcct ttcgtaatag cccgaccgca
     3241 gcggtcgcgc gcacagatag gcgcggcgga cgccgagcgc gaagcaagcc cgagttgcgc
     3301 ggtcgaccag cgccgcaccg actccgcgtt ggcgtgcctg cggatcgacc cacaccgcgg
     3361 cgacccatgg tgtcaattgc gggcgctcgg cgagatcgga tgcgatcacc gatgtcgtac
     3421 caagaaaaac ttcgccgtca tgggcgacca acgcaaacgg aatcgccgcg gcactcaggt
     3481 tctcccgcag acggcccgaa atatattcga gcggatggcc gtccgccttc caccaggcct
     3541 gccagatccg gtcggcaaca gaatcgaaaa actccgggcg ctgccgcagg tcggagatcg
     3601 tgaaggcaag aggcatggta taatctattg gctatcgctg gtcgggagcg atgtcttact
     3661 tcaccctgcg tttaacggga agctgttcaa atcttcaggc gcgcggccgc aatttgcgtt
     3721 aaatccagtc gcgccgcatg ctcgccaccc gtgctgattg atcgagagat ccaatgacag
     3781 aacgactgct caccaaaaag accgtcgatg tgctgggtag ccgcatggcc tatcacgagc
     3841 gcggcgaggg aacgccggtg ctgtttctgc atggcaatcc gacctcgtcc tatctttggc
     3901 gcgacgtcat tcccgaactg gaaggatgcg gccgcctgat cgcgccggat ctgatcggca
     3961 tgggcgattc cgccaaactg ccaaatcctg ataccgacac ctaccgcttc acgacccacc
     4021 gcaaatatct ggcgggcttc atcgatgcgg tcatcggtcc cgcgcaaccc atcgtgttcg
     4081 tggtgcacga ctggggctcg gcgctcggct tcgactgggc caaccatcac cgcgatcgcg
     4141 tccgcggcat cgcctatatg gaggggatcg tgcggccggt cgccggctgg gaggaatgga
//
LOCUS       DASWGM010000807        12978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_102478,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000807 DASWGM010000000
VERSION     DASWGM010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102478"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VGA15_22270"
     CDS             <1..281
                     /locus_tag="VGA15_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009495311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HEX9590458.1"
                     /translation="YFGFCETPSVLRELDQWIRRRLRAVVWKQWRRGPTRFAELRRRG
                     VGPVLAAQAAGSPRGPWRISISPALSIALSNAFFTSLGLATVTVPNAV"
     gene            complement(381..797)
                     /locus_tag="VGA15_22275"
     CDS             complement(381..797)
                     /locus_tag="VGA15_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9590459.1"
                     /translation="MVVKVNALDHIVINVADVARSAQWYQKVLGMEIGVFDPGPGQTP
                     RTSLMFGNQKINVRPRDADKVEWFTADHETAGSDDLCFLTASTPDQVVAHLTACGMAI
                     EEGPVARQGARGTLRSVYCRDPDGSLIEISSYEDGA"
     gene            932..1906
                     /locus_tag="VGA15_22280"
     CDS             932..1906
                     /locus_tag="VGA15_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9590460.1"
                     /translation="MASRRTAICLFAMGISAIASIGSASAVDYPTRPVRFVVGYPPGG
                     ATDIIARLIGQRLSEKLGQQFVIENKPGAGNNIGTEAVVNAEPDGYTVLLINPANYIN
                     ASLYGNLKFNFVRDIAPVASFNRVPNVMTVAPDVPAKTVAEFIAYVKANPGKVNMASS
                     GNGTSVHLSGELFMAMSGTKMQHVPYRGAAPAITDMLGGQVQVIFDNMPSIIQHIRSG
                     ALRALAVTTAERSSQLPDAPALAETVPGYEASALFGMGVPRKTPPEIVEKLNKEINAV
                     LAEPDMKKRLTELGGEPLISTPDAFGAMIVAETEKWEKVVKFAGAHVE"
     gene            1992..2252
                     /locus_tag="VGA15_22285"
     CDS             1992..2252
                     /locus_tag="VGA15_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HEX9590461.1"
                     /translation="MRKAMLALLVLSAAGAATVAGSGPAAAFDYPYCLQGRGVGVPGD
                     CSYQSYAQCMASASGRAVYCNINPRVAFGQQRHGRPYRNYYN"
     gene            2310..2765
                     /locus_tag="VGA15_22290"
     CDS             2310..2765
                     /locus_tag="VGA15_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9590462.1"
                     /translation="MVKYENPALDRTFAALADPTRRALLARLGRQASISVSDLAQPFA
                     MSLPAIMKHLDVLSDAGLIARAKTGRTVACRLTAKPMAQAMDWLGHYQRFWSEGLDRL
                     AAFVEEDQWHPNQAPPAMPVSSEDRASPSNVVSMRRRRKSTPRGPTRKK"
     gene            2765..3106
                     /locus_tag="VGA15_22295"
     CDS             2765..3106
                     /locus_tag="VGA15_22295"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX9590463.1"
                     /translation="MRWFGRSDAKPGSFQAEIDARIGGRYRVSFSTDDEYYEVGGIYR
                     EVVPNQKLVFSWAWHSTPERESLVTVSLKPDGDGTLLTLTHEQLFDQAARDGHERGWL
                     GALDKLERFVA"
     gene            3132..3869
                     /locus_tag="VGA15_22300"
     CDS             3132..3869
                     /locus_tag="VGA15_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX9590464.1"
                     /translation="MQPHSIVSREEWIAARKAHLAHEKEYTRARDRLSEERRALPWVK
                     VDKSYVFDGPNGKQTLGDLFAGRNQLVVQHFMFAPDWNEGCKSCSFWADGFERMIPHL
                     AARDTTLVAISRAPLEKLDAFKQRMGWTFDWLSSAGNDFNYDYAVSFTPQEIAGGAKV
                     YNFGTSGFGIEEAPGISVFFRDEAGNIFHTYSCFARGLDMMNAAYHYLDLTPLGRHEE
                     GLPYPMDWLRLRDQYQPAAGAAACCHT"
     gene            complement(4038..4232)
                     /locus_tag="VGA15_22305"
     CDS             complement(4038..4232)
                     /locus_tag="VGA15_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590465.1"
                     /translation="MNGTLSLAELDDRIAILRDNLRQLVEQAAAASGSQNEERIADRI
                     TQQTEELDRLILERDSRAKK"
     gene            complement(4382..4576)
                     /locus_tag="VGA15_22310"
     CDS             complement(4382..4576)
                     /locus_tag="VGA15_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590466.1"
                     /translation="MSERLETLRKARERMTDDRDAFAKTLAAPFDREKAERARIKFIE
                     TQVLIDAIDRAIEGERSEAA"
     gene            complement(4682..4879)
                     /locus_tag="VGA15_22315"
     CDS             complement(4682..4879)
                     /locus_tag="VGA15_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590467.1"
                     /translation="MRVSLVGVAVGEFQSASVGLGYLIQYGSRIFRLDIVMTAVTVLA
                     VVSSLIYGAIVWAERFVSRRR"
     gene            5112..5822
                     /locus_tag="VGA15_22320"
     CDS             5112..5822
                     /locus_tag="VGA15_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX9590468.1"
                     /translation="MHPYEIPAAVKERVVNRMGRLTVNETIDASRCAFIVIDMQNYFC
                     AEGFPAEVPMARTIVPNINRLAEAVRLAGGIVGWIQTTAAGAVDQWHNYQTEMLTPDR
                     QRARLAGLDEASEGFKLLPALDVRSDDLRLKKVKYSAFIQGSSGIDAALKTRNVDTVL
                     IAGTMTNVCCESSARDAMMLGYEAALISDCNASLTDEEHGAALNTFMMFFGDVMTSDE
                     ARWGPVGPAPSPLIYARS"
     gene            complement(6064..6140)
                     /locus_tag="VGA15_22325"
     tRNA            complement(6064..6140)
                     /locus_tag="VGA15_22325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6104..6106),aa:Arg,seq:ccg)
     gene            complement(6290..6544)
                     /locus_tag="VGA15_22330"
     CDS             complement(6290..6544)
                     /locus_tag="VGA15_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590469.1"
                     /translation="MSKPFCDESHPCPLSRPARNGRWPALGFPTNCVDRSPDLVAVDL
                     PAGFTIAEAPVGVEMVVSKVGYAFEQATHHRLPPQSMPGL"
     gene            6543..6842
                     /locus_tag="VGA15_22335"
     CDS             6543..6842
                     /locus_tag="VGA15_22335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX9590470.1"
                     /translation="MLTISAVIRVKKGHEDTMRRALLEVAENVRVNEPGTIGFFVSQE
                     QENPSVFTTYERFLDQAAMDAHNDSQVVARFFGIAKPILDGDVILVTSTEISAKA"
     gene            complement(6907..7542)
                     /locus_tag="VGA15_22340"
     CDS             complement(6907..7542)
                     /locus_tag="VGA15_22340"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX9590471.1"
                     /translation="MQVAVLTFDGFNELDSFVSYLIINRLKAKGWQAFITSPNEHVAS
                     MNGINIQAEKPLEFAQEADAVLVGSGTKTLDIVNDSSLMSRIKLNPDIQLIGAQCSGT
                     LVLSKLGVLDTGVACTDLKTKPWVIESGLDVLDQPFFADGNVATAGGCLASQYLATWV
                     IMKLGSQRDVEAALRTVAPVGQIEEYISRALSAVAPYIRSSSEVSAIADVV"
     gene            7779..8858
                     /locus_tag="VGA15_22345"
     CDS             7779..8858
                     /locus_tag="VGA15_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9590472.1"
                     /translation="MRPLEFGWYLPTHGDTTAYGVMEAQVAGSPELCDRVVQAAEAAG
                     FEYLLIPVGSTCWEAWISGAFMAARSARIKPLIAARPGYINPVLLAKMISTFDQMSGG
                     RICVNLIAGQNESEVEGEGVRYAKEERYVLMEEEVSILKALWTTRGPLNFAGKFHTLS
                     GAHIRPRPLQRPFPKFYLGGGSRQAWEMSAKHSDVHLFWGDLPERIADNIAEIREMAK
                     AHCRDNDIGFGMRLQVICREDEKDAWEAADTLVRNATERQKQEMKTLYNNSAANQRVQ
                     QLAREHGDLLLPHLWTGITRVRPGAGIAVVGNPRQCADTLQQFIDAGCHSFCLSGYLH
                     DEEAERFGRLIRPILAETNRGRLVA"
     gene            complement(8931..9866)
                     /locus_tag="VGA15_22350"
     CDS             complement(8931..9866)
                     /locus_tag="VGA15_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX9590473.1"
                     /translation="MQPPDRKIILVVRETRLDELIARFNTVQQAQFYVEHLGADFGDY
                     LEEQRQLHAATREVETSLRGFGRVQRLDRRYLGNFIFGADDIVVVLGQDGLVANTLKY
                     LDGQHVLGVNPDAKRWDGVLLPFRPDDLPRIMLEEVARRRPLKRVTMAKASLNTGETL
                     YAVNDLFVGPRSHISARYTLRVDDREEQQSSSGIIVSTGMGSTGWLKSLYASWAGAAA
                     SFDIAPPSRALDASFPWDSEFLHYFVREPFPSRTTGASLVSGRIPAGSAATIVSGMAE
                     HGVIFSDGIEADFLEFNAGARATIGIAERQGLLVV"
     gene            complement(9870..10889)
                     /locus_tag="VGA15_22355"
     CDS             complement(9870..10889)
                     /locus_tag="VGA15_22355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016735380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9590474.1"
                     /translation="MLGFRFIKTEPTQYVIQYRNGRPVREGAGLAFWYFAPSTSMIVV
                     PTASVNEPFIFPEITSDFQEVTIQGQITYRVTDPRRTAALLNFTFGPKGSYLSEDPKK
                     LSQRLINQVQVAMRAEVQALSLKQVLASGEPLVERVAGLLQDHPTVKTLGLELLGLSL
                     LAVKPRPETAKALEAEAREALLRRADEAIYTRRNAAVEQERTIKENELATEIAVENKK
                     RQVREAQMEAERSVQTRRLQMQQEEMTGKIGLEEQNKALVILASANAREEADAQAYGL
                     AAMMKSFGDADPKILQALASAGMSPGQLMALAFRDLADNATKIGQLNVTPDLLREMLQ
                     PQTEH"
     gene            11032..11736
                     /locus_tag="VGA15_22360"
     CDS             11032..11736
                     /locus_tag="VGA15_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007697570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9590475.1"
                     /translation="MDSAPNPKDFASKPIASVDLVIFALSETGLQVLLMKRGTEPFAD
                     HWALPGGWIHVDEDTDLDTAARRVLFDKTGVETPYLEQLRTFGGKSRDPRGWSLSIAY
                     VALISAHEVRLRQGSNATDAAWWSIEGNEVAVLTAFDHSNIFHSALARLRDKVEYSTL
                     PVHLLPAGFTLGELQSVYERILGRRLDKSAFRKRVAEADFVEPVPGEMRRASNRPAQV
                     YRLKKARSTTFFDRTI"
     gene            complement(11931..>12978)
                     /locus_tag="VGA15_22365"
     CDS             complement(11931..>12978)
                     /locus_tag="VGA15_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590476.1"
                     /translation="WAQVRRGSDQGIGGTGITRGHDQGIGGTGIVGVIQRFGSIFVNG
                     ERISYAPNVVVRIDGEPASAMALKIGHLARVLALPQANGSLITHGINVVSEVMGPIES
                     VKDGEMTVLGQRIIAAGHESWRHPGTNVAVFGLRRTDGVIVSSLVEQRRDATTRVSGP
                     LERDRDGLRIGGLRLNGVDAALVGRRVQVEGRVTQGVMQVSRIRRDDLSDFPGASRLS
                     IEGYVRRVGRDLQFGSGYVAHDDGRFKSSGKARVVVNAVFDGSGSLRVESVQSVNKFP
                     GESMRGTRPERTPGATPEPGGGSNVPRGDPTVPDSGPPGLGGGGPPGLSGGSPPGLGG
                     GGPPGLGGRGGGRR"
BASE COUNT         2488 a         4021 c         3975 g         2494 t
ORIGIN      
        1 gctacttcgg cttctgcgag acaccgtcgg tcttgcgtga acttgaccaa tggatcaggc
       61 gacggctgcg tgccgtcgtc tggaagcaat ggagacgcgg acccactcgc tttgctgaac
      121 tgcgacgccg cggcgtcggc ccggttctgg cggcgcaagc cgctggcagc ccacgtggcc
      181 cttggcggat cagcattagc ccggcactct ccatcgctct atcaaacgcc ttcttcacct
      241 cactcgggct tgcaactgtc acggtaccaa atgctgttta attcatcgaa ccgccgtata
      301 cggacccgta cgtacggtgg tgtgggaggg gaggagccgt gaggttcccc cctatcccga
      361 tcgcaatgac ggagagagga ctatgcacca tcctcatacg acgaaatctc gatcaagctg
      421 ccgtcgggat cccggcaata gaccgagcgc agcgtgccgc gggcgccttg tcttgccacc
      481 ggaccttcct cgatcgccat gccgcacgcg gtcagatgcg ccaccacctg atccggcgtg
      541 ctggcggtga gaaagcacag atcgtcgctt cctgcggttt catgatccgc cgtgaaccac
      601 tcgaccttgt cggcatcccg cggccgcaca ttgatcttct gattgccgaa catcagcgac
      661 gtccgtggcg tttggcccgg gcctggatcg aacaccccga tctccatgcc gaggactttt
      721 tggtaccact gcgcggaacg tgccacgtcg gcgacattga tgacgatatg atcgagcgca
      781 ttgactttga caaccatgga ttatcctttc gtcgcggtcc aggcgcatgt gcgcgactag
      841 ccgcatcagg ctttgtttgt tacaacgccc tcctttgtca acttcaacaa gaacggacgc
      901 gcgttgcaga tgtccgtcag ggagaaactg catggcttca cgccgcaccg cgatttgcct
      961 gttcgcaatg ggtatttcgg caatcgcttc gatcgggtct gcttcggcgg tggattatcc
     1021 gacccggccg gttcgcttcg tggtgggata tccgcccggc ggcgcgacgg atattatcgc
     1081 gcggctgatc ggccagcggc tgtctgaaaa actcggccag caattcgtca tcgagaacaa
     1141 gcccggcgcc ggcaacaata tcggcaccga ggcagtggtc aatgccgagc ccgacggcta
     1201 caccgtgctg ctgatcaatc ccgccaacta catcaacgct tcgctttacg gcaacctgaa
     1261 attcaacttc gttcgcgaca tcgcgccggt ggcgtcgttc aatcgcgtgc cgaatgtcat
     1321 gacggttgct ccggatgtgc cggcgaaaac ggtcgccgaa ttcatcgcct atgtgaaggc
     1381 caatccgggc aaggtgaaca tggcgtcatc gggcaacggg acctcggtgc atttgtccgg
     1441 cgagctgttc atggcgatgt cgggcaccaa gatgcagcat gtaccctatc ggggcgccgc
     1501 acccgcgatc accgacatgc ttggcggcca ggtccaggtg atcttcgaca acatgccctc
     1561 gatcatccag cacatccgct ccggcgcctt gcgcgcactg gcggtgacga cggcggaacg
     1621 ctcttcacag ttgccggacg cgcccgcgct cgccgagacc gttccgggct atgaggcgag
     1681 cgcgctgttc ggcatgggcg taccaaggaa gacgccgccg gagatcgtcg agaaactgaa
     1741 caaggagatc aacgccgtgc tggccgaacc ggacatgaaa aaacgcctga ccgaactcgg
     1801 cggcgaaccg ctgatcagca cgccggacgc ttttggcgcg atgatcgtgg ccgagaccga
     1861 gaaatgggag aaggtggtca aattcgccgg cgcccatgtg gaatagggta cggccggaac
     1921 attcttaagt tggcctcgtt ttgaacgtac gacgagcaag ccgcgaccaa tgcgaagaag
     1981 gagacatgac tatgcgcaaa gcaatgttgg cactgttggt tttgtcggcg gccggtgccg
     2041 ctaccgtggc gggctctggt cccgccgccg cgttcgatta tccctattgc ctccagggcc
     2101 ggggtgttgg cgttcccggc gactgttcct atcagtccta cgcgcaatgc atggcgagcg
     2161 cgtccggacg agccgtctat tgtaacatca atccgcgcgt cgcctttggc caacagcggc
     2221 acggacggcc ttatcggaat tattacaact agtgattttc gccggcgggc gggcttgact
     2281 aacgttcggt cgcacgtata ctaaaccaca tggttaagta tgagaatccg gcgctggacc
     2341 gcacctttgc ggcactagcc gacccgaccc ggcgcgcgct gctggcgcgg ctcggcaggc
     2401 aagccagtat ttcggtgagc gatctggcgc agccgttcgc gatgtcgctg cccgcgatca
     2461 tgaagcatct cgacgtgctg tcggacgcgg gcctgatcgc gcgcgcgaaa accggccgca
     2521 ccgtggcatg ccggttgacc gcaaagccga tggcgcaggc gatggactgg ctcggccact
     2581 accagcgctt ctggtccgaa ggtctcgacc gccttgccgc ttttgtggag gaagatcaat
     2641 ggcacccaaa tcaagcgccg ccggcgatgc cggtctcgtc ggaagacaga gcctcacctt
     2701 caaacgtcgt ctcaatgcgg cgccggcgaa agtctacgcc gcgtggaccg acccggaaaa
     2761 aataatccgc tggttcggac gctcggacgc caagcctggc tcgtttcagg cggagatcga
     2821 cgcgcggatc ggcggccgtt accgcgtcag cttctccacc gatgacgagt attacgaagt
     2881 cggcggtatc taccgcgagg tggtgccgaa ccagaaactg gtgttcagct gggcgtggca
     2941 ttcgacccct gaacgcgaat cgttggtcac ggtctcgctg aaacctgacg gtgacggcac
     3001 gctgctcacg ctgacccacg agcagctgtt cgatcaggcc gcgcgcgatg gccacgagcg
     3061 cggatggctg ggagcgctcg acaagcttga aagatttgtc gcctgaagca ggtccgacaa
     3121 aggagccagt catgcaaccg cacagcatcg tctcgcgtga agaatggatc gccgcgcgca
     3181 aagcccatct cgcgcatgag aaggaataca cgcgcgcgcg ggaccgtttg agcgaagagc
     3241 gccgcgcgct gccctgggtc aaggtcgaca agtcttacgt gttcgatgga ccgaacggca
     3301 agcagacgtt gggcgatctg ttcgccggac ggaaccagct ggtggtacag catttcatgt
     3361 ttgcgcccga ctggaacgaa ggctgcaaga gctgctcgtt ctgggcggac ggcttcgagc
     3421 gcatgattcc gcatctggcg gcgcgcgaca ccacgctggt cgcgatatcg cgcgcgccgc
     3481 tggaaaaact tgatgcgttc aagcagcgaa tgggctggac cttcgactgg ttgtcgtccg
     3541 cgggtaacga tttcaactac gactacgccg tctcgttcac gccgcaggaa attgcggggg
     3601 gcgccaaggt ctataatttc ggaacctccg gcttcgggat cgaggaggcg cccggcatca
     3661 gcgtgttttt ccgcgacgag gcgggcaaca tcttccacac ttattcgtgc ttcgcgcgcg
     3721 gcctcgacat gatgaacgcc gcctatcact atctcgactt gacgccgctc ggacgccacg
     3781 aagagggctt gccatatccg atggactggc tgcggctgcg cgaccagtat cagccagccg
     3841 cgggtgccgc cgcgtgctgc cacacgtgag aagcgtttga aatcgtcatt gggcgcgcat
     3901 tcgcgcgtcc cgatggcttg caatcgatga acaaagagag aagcgcgaat gccttacgcc
     3961 ggtggcttca tcgccgcggt cgcgaagaag aaccttgccg caccttgacg ctgatcggcc
     4021 acggcagcca gacagcgtca tttcttcgcg cggctatcgc gctccagaat cagcctatcg
     4081 agttcctccg tctgctgggt gatgcggtcc gcgatcctct cttcgttctg gctgccggat
     4141 gccgccgccg cttgctcgac aagttgccga aggttgtcgc gcaggatcgc aatgcgatcg
     4201 tcgagttccg caagcgatag tgtgccattc atcgtcgctc tccttgccga caccgttgcc
     4261 cgtcaaccct tgctcaggct tcaccacgct ccaggcgggc tgccgccctg attttgctgg
     4321 cccccattct cagccaatat cgctacctgc tgcaagcgga agctcgattc gaactggacg
     4381 gtcaggcagc ctcggaccgt tcgccctcga tggcccgatc gatggcgtcg atcagcacct
     4441 gggtctcgat gaatttgatc cgcgcccgct cggccttctc gcgatcgaag ggtgcggcca
     4501 gggttttggc gaaggcgtcg cggtcatcgg tcattcgttc gcgcgctttt cttaatgtct
     4561 caagtcgctc gctcatatcg gctccgcttt ctgttggtcg cgtgatcttg gttggcgcga
     4621 aatgcaattg gtacaaacgc atgacgaccg gatcggctgg tcggatgtcg ccggcggcag
     4681 gctatcgccg gcgcgagacg aacctctcgg cccagacgat ggcgccataa atcagcgacg
     4741 ataccaccgc gagaactgtc accgccgtca tgacaatatc aagccggaat atccggctgc
     4801 cgtactgaat gaggtagcca aggccgacgc tggccgactg aaactcgccg acggcgaccc
     4861 ccaccagcga aacccgcatt gaccttgagc gccgcgatca ttgtgggaat gctaccgggc
     4921 agaatgacgt tggtcaggat ctgcgcctcg ctggcgccaa acgttcgtgc gagcttgatt
     4981 tggttgactt caacaccctt aaagccactg tagatcacaa ggatcgtgac gaacagggaa
     5041 aatcgcaagc gacatcgcat agatcgattg cgacgggatc caaagactag gttttttaag
     5101 gagacgcgtc catgcacccc tacgaaattc cggctgccgt aaaggaacgc gtcgtcaacc
     5161 ggatgggaag gctgacagtc aacgaaacca ttgacgccag ccgctgcgcg ttcatcgtca
     5221 tcgacatgca gaattacttt tgcgccgaag gctttccggc cgaagttccg atggcgcgca
     5281 cgatcgtgcc aaatatcaat cggctcgccg aggcggtgcg tctcgccggc ggcattgtcg
     5341 gatggatcca gaccacggcc gccggtgcgg tcgatcagtg gcacaactat cagaccgaga
     5401 tgctgacgcc ggatcggcaa cgcgcgcggc ttgccggtct cgatgaggct tccgaaggct
     5461 ttaagctgtt gccggcgctc gacgtgcgta gcgacgatct ccggctcaag aaagtcaagt
     5521 acagcgcctt catccagggc tcgtccggca tcgacgcggc gctgaagacg agaaatgtcg
     5581 ataccgtcct gatcgccggt acgatgacca atgtgtgctg cgaatcatcc gcgcgcgatg
     5641 cgatgatgct cggctacgag gccgcgctga tctcggactg caatgcctcg ctgaccgacg
     5701 aagaacatgg cgctgcgctc aatacattca tgatgttttt cggagacgtc atgaccagcg
     5761 acgaagcaag gtggggaccg gttggtcccg caccgtctcc gctgatctac gcaagaagct
     5821 gaatgaaccg gctgctcagt tccgaactgc tccagttcaa gccgctcggg aggaagccat
     5881 ccaagatccg cgcgaattct tgttcgaatc cgccgcagac agcgagcacg gacgagcgat
     5941 gcgttcgagc tatgagtcga gatcagcgcc gataccccgc caatcttccc atatttttgg
     6001 taagtttgtg gtgagttttg ctttcactca cgtccggacg ctcaggctaa gtcattgatt
     6061 ttatggcgca cccgacacga ttcgaacgtg tgacctttgc cttcggaggg caacgctcta
     6121 tccagctgag ctacgggtgc ttggggggtt catttagccg attggcctga cctcggcaac
     6181 ggccaaacca gggctgtttt gggcgagcag caaggcccga aaaggcgagg gtcacgcgct
     6241 cgaatcgcgt cgggtgcgcc agaaagagca ccatccgtag ttgcaaaagt caaagcccag
     6301 gcatgctctg cggcggcagg cggtgatggg tggcctgctc gaaagcatag cccactttgg
     6361 acaccaccat ctcgacgcct accggtgctt ccgctatcgt aaagccggcc ggcagatcga
     6421 cggcaaccag gtccgggctg cgatcgacgc aattggtcgg aaagccaagc gccggccagc
     6481 ggccgttgcg cgcgggtcgg ctcagcgggc atgggtggga ttcatcacaa aagggctttg
     6541 acatgctgac catcagcgcc gtcatccgcg taaaaaaagg tcacgaagac accatgcgcc
     6601 gcgctttgct cgaggtcgcc gaaaacgttc gcgtcaacga gccgggcacg atcggtttct
     6661 tcgtttcaca ggaacaggag aacccttctg tcttcaccac ctatgagcgg ttcctcgatc
     6721 aggccgccat ggacgcccac aacgattcgc aagtcgtggc gcgcttcttc ggcatcgcaa
     6781 agcccattct cgacggcgac gtcattctgg tcaccagcac cgagatttcc gccaaggcct
     6841 gaccttgaag gcctgacctt gaaggcctga ccttggcaac agcgggaatg ccaggcagcg
     6901 cagccgtcac acgacgtccg caatcgcgga gacctcggaa gaactgcgaa tgtagggcgc
     6961 gaccgccgaa agcgcacgcg agatatactc ctcgatctgg ccgaccggcg cgacggtcct
     7021 caaagcggct tccacgtcac gctgcgatcc gagtttcatg atgacccatg tggcgagata
     7081 ttgagacgcc aggcatccgc cggcggtggc cacgtttccg tccgcgaaga acggttgatc
     7141 gagcacgtca aggccgcttt cgatgaccca gggttttgtc ttcaggtcag tgcatgccac
     7201 gcccgtgtca agaacgccga gcttcgacaa aaccagcgtt cccgaacatt gggctccgat
     7261 cagctgaata tccgggttca acttgatgcg cgacatcaag ctggaatcat tgacgatgtc
     7321 caacgtcttt gtcccgcttc cgaccagcac ggcgtccgcc tcttgcgcga actcgagcgg
     7381 cttctcggcc tggatattga tgccgttcat cgatgcgacg tgctcgttcg gcgaggtgat
     7441 gaaagcctgc catcccttgg ctttaagtct gttgatgatc aggtaggaca caaaggagtc
     7501 cagttcattg aagccgtcga acgtaagaac tgcgacctgc atcttctgct ccgtgaaaat
     7561 tgcgacgtaa tttactctat ccagccgagc ttcgggggtg tcgggttcat tttagccgat
     7621 tggcctgatg tcgggaaagt ccctgcaccg ctcgcagggc gccacgcaag gcccgaagtg
     7681 gcggagccgg cagcctgatt gtcagggagg acgcagcctg taaactaccc ctcgagggaa
     7741 ttgcgttgcg agagccgcgc ttcaacggag aaaagtccat gcggccgctc gaatttggct
     7801 ggtatctgcc gacgcatggc gacaccacgg cctatggtgt gatggaggct caggtggcgg
     7861 gatcgcccga actgtgcgac cgtgtggtgc aggccgcgga ggcggcggga ttcgaatatc
     7921 tcttgatccc ggtgggttcg acgtgctggg aggcatggat atcaggcgcg ttcatggcgg
     7981 cgcgttcggc caggataaaa ccgctgatcg cggcccggcc cggctacatc aatccggtgc
     8041 tgctggcgaa gatgatctcg accttcgacc agatgtcggg cggccgcatc tgcgtcaacc
     8101 tgatcgccgg ccagaacgaa agcgaagtcg aaggcgaggg cgtgcgttac gccaaggaag
     8161 agcgctacgt gctgatggag gaggaggttt cgatcctcaa ggcgctgtgg accacgcgcg
     8221 gcccgctgaa tttcgcaggc aaattccaca cgctgtcggg cgcgcacatc cggccgcgac
     8281 cgctgcagcg gccattcccg aaattctatc tcggtggcgg ctcgcgtcag gcctgggaaa
     8341 tgtcggcaaa acattccgac gtgcatctgt tctggggcga tctgccggag cggatcgctg
     8401 ataacatcgc cgagatcagg gaaatggcga aagcgcactg ccgcgacaac gacatcggat
     8461 tcggcatgcg gctgcaggtg atctgccgcg aggatgaaaa ggacgcgtgg gaggccgccg
     8521 atacattggt gcgcaacgcg accgagcggc agaagcagga gatgaagacg ctctataaca
     8581 attccgcggc caatcagcgg gtgcagcagc tcgcccgcga acatggcgat ctgctgctgc
     8641 cgcatctgtg gaccggcatc acccgggtcc ggcccggtgc cgggatcgcg gtggtcggca
     8701 accccaggca atgcgccgac acattgcagc agttcatcga tgccggctgt cattcgtttt
     8761 gcctgtcggg ctatctgcat gacgaggaag ccgagcgctt cggacggctg atccgtccaa
     8821 tcctcgccga gaccaatcgc gggcggctgg tagcgtagcg ccgtggccgg tcggatgtga
     8881 cgctgtgtaa gccaatctgt ccgaatgcgc atccaggatc gggccggcca tcacaccacc
     8941 aacagtccct ggcgttcggc aatgccaatc gtcgcccgcg cgcctgcgtt gaactcgagg
     9001 aaatcggcct cgatgccgtc actgaagatc acgccgtgtt cggccatccc ggagacaatc
     9061 gtagcagcac taccggctgg aattcggccg gataccagcg aggcgccggt ggtccggctc
     9121 gggaatggtt cgcgaacgaa gtagtgcagg aactccgaat cccagggaaa tgaggcgtcc
     9181 agggccctgc tcggtggcgc gatatcgaag ctggcggcgg caccagccca gctggcatac
     9241 aggctcttga gccatccggt cgaccccatc ccggtcgaga caatgatccc gctcgaggat
     9301 tgctgttcct cgcgatcgtc gacgcgcagc gtatagcggg ccgagatgtg gctgcgaggg
     9361 ccgacgaaga gatcgttcac cgcatacagt gtctccccgg tattgagcga cgccttcgcc
     9421 atggtgacgc gcttgagtgg ccgtctgcga gccacctcct caagcatgat cctggggaga
     9481 tcgtcagggc gaaacggcag cagcactcca tcccagcgct tcgcgtccgg gttaacgccg
     9541 agcacatgct gaccgtcgag atatttgagc gtgttcgcca ccagcccatc ctgtcccagc
     9601 acgacgacga tatcgtcggc cccgaagatg aaatttccga gatagcgtcg atcgagccgc
     9661 tgaacccggc cgaagccgcg aaggctggtc tctacctcgc gcgtggcggc gtgcagctgt
     9721 cgctgctcct cgaggtagtc gccgaaatcg gccccgagat gctcgacata gaactgcgcc
     9781 tgctggaccg tgttgaaacg ggcgatcagc tcgtcgagcc ttgtttcgcg caccaccaat
     9841 atgatcttgc ggtcgggggg ttgcatggct caatgctcgg tttgaggttg cagcatctcg
     9901 cgcaggagat ccggggtgac gttgagctgg ccgatcttgg tcgcattgtc ggcgaggtcg
     9961 cggaaagcga gcgccatgag ctgccccggg ctcatgccag ccgaggcgag cgcctgaaga
    10021 atcttcggat cggcgtcgcc gaacgatttc atcatcgcgg ctagtccata ggcctgggca
    10081 tccgcctctt cgcgggcatt ggccgaagcc aggatgacga gcgccttgtt ctgctcctcg
    10141 agcccgatct tgccggtcat ttcctcttgc tgcatctgca gccggcgcgt ctgcaccgaa
    10201 cgctccgcct ccatctgggc ctcccgtacc tgccgcttct tgttttcgac cgcgatctcg
    10261 gtcgcgagtt cgttctcctt gatggtccgt tcctgttcga cggcggcgtt gcggcgggtg
    10321 tagatggcct cgtcggcgcg ccgcaacagc gcctcgcggg cttccgcttc gagcgccttg
    10381 gcagtctcag gccgcggctt gaccgcaagc agcgaaaggc cgagcagttc gagaccaagc
    10441 gttttgacgg tcgggtgatc ctgcagcagg cccgcaaccc gttccaccag cggctcgccc
    10501 gaggcaagaa cctgcttcaa tgacaacgct tgaacctcgg cgcgcatcgc gacctgcacc
    10561 tgattgatca gccgctgcga aagtttcttc ggatcctcgg agaggtagct gcccttcggc
    10621 ccgaacgtga agttgagcag tgccgcggtg cggcgagggt cggtgacgcg gtaggtgatc
    10681 tggccctgga tcgtcacttc ctggaagtcg gaggtaatct ccggaaaaat aaagggctcg
    10741 ttcacgctcg cggtcggcac cacgatcatc gaggtcgacg gcgcgaaata ccagaaggcc
    10801 aggcctgcgc cctcccgcac gggacggcca tttcgatact ggatgacgta ttgcgtgggt
    10861 tcggttttga tgaagcgaaa tcctaacatg cctggttccc tttctgttca catggcatcc
    10921 gaggccttgc gtatgggcat caaactccac ttagtgtaat tcgtcaacta agttagttgt
    10981 cgcttcacac taaggcaaag gcacgctact ttgtcgtcca tgaccgcgtt tatggattct
    11041 gcccccaacc ccaaggattt cgcctcaaag ccgatcgcgt cggtcgacct tgtcatcttc
    11101 gcgctgtcgg agacgggcct tcaggtgctg ctcatgaaac gcggcaccga gccgttcgcg
    11161 gatcattggg cgctgccggg cggctggatt catgtcgacg aggacaccga tctcgatacc
    11221 gccgcgcgcc gcgtgctgtt cgacaagacc ggggtcgaga caccctatct ggagcaattg
    11281 cggaccttcg gcggcaaatc ccgcgacccc cgtggctgga gcctcagtat cgcctatgtg
    11341 gcgctgatct cggcgcacga ggtgcggctg cggcagggat ccaatgcaac cgacgccgcc
    11401 tggtggtcga tcgaaggaaa tgaggtcgcc gtactgacgg ccttcgatca ttcgaacatt
    11461 ttccacagcg cgctcgcccg gctgcgggac aaggtcgagt actcgacgct ccccgtgcat
    11521 ctcctgccgg ccgggttcac ccttggcgaa ctgcaatccg tctatgaacg gattctcggt
    11581 cgccggctcg ataaatcggc gttccgcaag cgtgtcgccg aggcagactt tgtcgagcca
    11641 gttccgggcg agatgcgacg cgccagcaat cggcctgctc aagtctatcg cctgaagaag
    11701 gcgcgctcga ccaccttctt cgaccgaacg atctgaaccc aggcagccgt gcctggagtt
    11761 catcggccag tggcaagcgt cgcgaaaagg ctcttcgagg ggtagatttt ctggtcaggc
    11821 aacggtcacc cgttcgaatc gtgtccggcg cgccacttcg gaatacgcca gcggggacag
    11881 ctccacgccg tcccgggtcg tgggccccac tggatccacc gcgactttgt tcagcgccgg
    11941 ccgccgccac gtccgcccag gccgggagga ccgccgccgc ccaggccggg aggactgcct
    12001 ccgctcaggc cgggaggacc gccgccgcct aggccgggag gaccgctgtc gggcacagta
    12061 ggatcaccgc gcgggacgtt cgagccgccg ccgggttcgg gcgtcgcgcc gggagttcgc
    12121 tcgggtctcg tgccgcgcat cgattcgccc ggaaatttgt tgaccgattg gacggattca
    12181 acccgcaagc taccggaacc gtcaaagacg gcgttcacga ccacgcgggc cttgcctgag
    12241 gatttgaagc gaccatcgtc atgcgcgaca tagcccgagc cgaattgcag atcgcggccg
    12301 acgcggcgga catagccttc gatcgacaga cggctcgcgc cggggaaatc agagaggtcg
    12361 tcacgcctta tgcgcgagac ctgcatcacg ccctgcgtaa cacgaccctc cacctgaaca
    12421 cgccgtccga cgagtgcagc atcgacgccg ttgagccgca agccgcctat tcgcaggccg
    12481 tcgcgatcgc gttcaagtgg tccggaaacc cgcgttgtag cgtcacggcg ttgctcgacg
    12541 aggcttgaga cgatcacgcc gtccgtccgg cgcaacccga agaccgcgac attcgtgccc
    12601 ggatggcgcc agctttcgtg gccggccgct atgatccgct ggcccagcac ggtcatctcg
    12661 ccgtccttga cgctttcgat cggccccatc acctcgctga ccacgttgat gccgtgggtg
    12721 atcagcgaac cgttcgcctg cggcagcgcc agcacgcgcg ccagatgtcc gatcttgagc
    12781 gccatcgccg atgccggttc gccatcgatc cgcaccacga cgttcggcgc atagctgatg
    12841 cgttcgccgt tgacgaaaat actgccgaaa cgctgaatga ccccgacaat gccggtgccg
    12901 ccgatgccct gatcgtggcc acgcgtgata ccggtgccgc cgataccctg gtcgctgccg
    12961 cggcgcacct gcgcccag
//
LOCUS       DASWGM010000808         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_102837,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000808 DASWGM010000000
VERSION     DASWGM010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102837"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VGA15_22370"
     CDS             <1..876
                     /locus_tag="VGA15_22370"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9590477.1"
                     /translation="GPDGAHSWHAMAYNPKTRLVYIPTQEIAKQFTPIEDFETAPIGW
                     NLGVDVAGTPGRKVGYLLAWDPVNQKEAWRVKYRGPWNGGVLTTAGNLVAEGDAAGNF
                     NLYRADTGEKLWSTFGQSGLMAGPVTYEVDGEQYIAVLSGWGSAFSLQAGKVAATSGN
                     LRNISRVLAFKLGGTTKLPALPPAEKLVLNPPPATADAATVATGKHLFGRFCSVCHGE
                     AAVGGGVVPDLRATAYLSDDTWYDILLNGALKDAGMVSFEAVLDRQKADAIRAYLIQR
                     ANDDKAASERKAADR"
     gene            1020..2348
                     /locus_tag="VGA15_22375"
     CDS             1020..2348
                     /locus_tag="VGA15_22375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX9590478.1"
                     /translation="MTRITRKGSEISKRDRIARRQFLRFLAGSPLLTATSAGAIAGLL
                     AARSHQVLAQSYDVLRGSRRALGPDGIITAPADALDVFEFEPAAKKAVLSPGAANGAA
                     PAHWGYLESGVDGDVTRDANHTAYTRYNIRVRRLIDARKVDTSVKIFGETWASPIFIC
                     PVSSLGAYDPEAGVAVARAAGKRNHQMMLSTVDNASIVDVNKAHGWPAWFMLYPTDDW
                     NVTRALVQRAESAGSPAIVLTVDRQGGRNTEELFRERRQDLRECTACHTPGFTNEVSR
                     KANFSGISVSKVTNLYGTGMTWEFVDRLREIVKGKLVLKGIMTGEDATEALRHGVDGI
                     QVSNHGGRAEESGQATIGVLVEVVDAVNGRVPVLIDGGVRRGTDVLKALGLGASAVGI
                     GRPYVWGLASFGEAGVDRVLQILDDEFITIMRQVGALNIKQITRENVTPA"
     gene            2373..2483
                     /locus_tag="VGA15_22380"
                     /pseudo
     CDS             2373..2483
                     /locus_tag="VGA15_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(2470..5064)
                     /locus_tag="VGA15_22385"
     CDS             complement(2470..5064)
                     /locus_tag="VGA15_22385"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX9590479.1"
                     /translation="MIKAGAGFGKTSLALSWAERLKQAGNLVAWLALDTDDDEPTRFL
                     FYVSQALRRACASVGESAIRLLSDVSLAPLNTIVSTLINDLADVENEMYLFLDDYHCL
                     THPDIHDAISFLLKHAPAQFHLVLTTREEPLLPLAVLRAQNRLFEIDVSALRFDPDEV
                     RHFLDQENLRTLAPEEVKVLHAKTEGWPAVLRIIASTSSQSTKDFGQYVRGLSGALRP
                     IGAYIAEMLEGLPPDMVQFMLRTAILDRLSAPLCQAVTGVKSSQAMLEAMEARQMLLA
                     PLDGEGRWYRYHALLAGHLSQKLQSDLGDEIPWLHRHAYRWYAAQELCRDAVHHAIAA
                     GDTDQAVAWIKNCAMALVKQGDLLTLLSWQRLFPTELMQSRLDVRLAIAWGMALAMRS
                     EEALTLAAGVEDDVDDTNAPAAEILRSECQAIRSVAIALKDDSQTSLPLALACLERTS
                     DPWTGNVASNVARFGHLKAGDLKSFYATPWIPYSSDDDRRNLFAAVYRRCLQGHAEAQ
                     QLRLNAAEHHYLDGMRLAEEHMGPQSIAAALPASLFARIRYEQGRIDESESLVIDRFP
                     LISGAGMLECVLSAYLVLVRVAQWRGNLERAHALLEQAETLGYGRSWGRLVAAALNEQ
                     LRLHLAQGRLTESGACLDRLDRLAAEYPSTAPCAWSDIRRYAMMARAQLVSAQKRPKD
                     SIAILKKLREEVAGRQDHYAALGLSIQLAVALLDADEPTQAMITFREVVSVAAPAGIH
                     QMILDGGPKVGTLLLRVLEHAQHTNSNGELLPYLQDILSSWRERYQKGPRPSSTLGIT
                     GLLSGRERNILELIGKGESNKEIARTLDIAPETVKSHVKNIFTKLGVEKRAQAVSRAQ
                     SFGLVSTH"
BASE COUNT         1025 a         1650 c         1686 g          965 t
ORIGIN      
        1 gggccagatg gcgcccatag ctggcacgct atggcctaca atccgaagac acgcctggtc
       61 tacatcccga cacaggagat cgcgaagcag ttcaccccga ttgaggattt cgagacggcg
      121 ccgatcggct ggaacttggg cgtcgatgtc gccggtacgc caggaaggaa agtgggttat
      181 ctcctcgctt gggatccggt gaaccagaag gaagcgtggc gtgtgaaata tcgtggccct
      241 tggaacggcg gcgtgttgac caccgccggc aatctcgtcg cggaaggcga tgccgccggc
      301 aatttcaacc tgtatcgtgc ggataccggc gaaaagctgt ggtcgacgtt tggccaatcc
      361 gggctcatgg caggcccggt cacctacgag gtcgacggcg agcagtacat cgccgtgttg
      421 tccggctggg gctcggcttt ctccttacag gcgggcaagg tggcggccac gtccggcaac
      481 ctacgcaata tcagccgcgt cctggcgttc aagctcggag gcacaaccaa actgccggcg
      541 ctcccgccgg cggagaagct ggtgctcaat ccaccgcccg ccacggccga cgccgcgacc
      601 gtcgccaccg gcaagcacct cttcgggcga ttctgcagcg tctgccacgg cgaggccgcg
      661 gtaggtggtg gggtggtccc ggatctacgc gccacagcgt acctatccga tgacacctgg
      721 tacgacatcc tcttgaacgg cgccctcaag gacgccggga tggtctcgtt cgaggccgtg
      781 ctcgatcgtc agaaggcgga cgcgatccgc gcctatctga tccagcgggc gaacgacgac
      841 aaggcggcga gcgaaagaaa agcggctgat cgctaaaaag gttaggggaa cggcaagctc
      901 caagactccg ttgctaggcg cgcggccggc aaggcaaggc tgtacctagt caggactggc
      961 gccgcgatcg aagcgaccaa ctgccgacgg ctttcttcgg catcacaatg gaggtactga
     1021 tgacccggat tacgcgcaaa gggtccgaaa tttcgaagcg cgataggatc gcgcggcgcc
     1081 agttcctgcg ctttctcgcc ggcagcccgc ttctcacggc gacctctgcc ggcgccatcg
     1141 ccggcctgtt ggcggctcgc tcgcatcaag tgctcgcgca gagctacgac gtgctgcgcg
     1201 gttctcgaag agcgctcggc ccggacggca tcatcaccgc tccggccgat gcgctggacg
     1261 tcttcgagtt cgagccggcg gcgaagaagg ccgtgctcag ccccggcgcg gccaacggcg
     1321 cggcgccggc gcactggggc tacctcgaga gcggcgtgga cggcgacgtg acgcgcgacg
     1381 ccaaccacac cgcctacacg cgctacaaca tccgcgtgag gcgcctcatc gacgcgcgca
     1441 aagtcgacac cagcgtgaag atcttcggcg aaacctgggc cagccccatc ttcatctgcc
     1501 cggtcagcag cctcggcgcc tacgacccgg aggcgggggt cgccgtggcg cgcgccgctg
     1561 gtaagcgcaa ccaccagatg atgctctcca ccgtcgacaa cgcgagcatc gtcgacgtga
     1621 acaaggcgca cggctggccc gcctggttca tgttgtatcc gaccgacgac tggaacgtga
     1681 cgcgggcgct ggtgcagcgc gccgagagcg cgggctcccc ggcgatcgtc ctcacggtcg
     1741 accggcaggg cggccgcaac accgaggagc tgttccgcga gcggcgccag gacctgcgcg
     1801 aatgcacggc ttgtcacacg ccgggcttca ccaacgaggt cagccgcaag gccaacttct
     1861 cgggcatcag cgtttccaag gtcacgaacc tctacggcac cggcatgacg tgggaattcg
     1921 tcgatcgcct gcgcgagatc gtcaagggca agctcgtgct gaagggcatc atgacgggtg
     1981 aggacgccac ggaggcgctg cgccacgggg tggacggcat ccaggtctcc aaccacggcg
     2041 gccgcgccga agagagcgga caggcgacga tcggcgtgct ggtcgaagtc gtggacgcgg
     2101 tgaacggccg cgtcccggtg ctcatcgatg gcggcgtgcg ccgtggcacc gacgtcctga
     2161 aggcgcttgg cctcggtgcc agcgccgtcg gcattggccg gccctacgtg tgggggctcg
     2221 cctcgttcgg cgaggccggc gtggaccgcg tgctccagat cctggacgat gagttcatca
     2281 cgatcatgcg gcaggtcggc gcgctgaaca tcaaacagat cacgcgggag aacgtgactc
     2341 ccgcatagcc cggccccgag ctctactgag ctcgggtcgc aactcgctat gacaagctcg
     2401 ccgccaatta tctcgccttt atccaactcg cgtcgatcag gctaggctgc gcgttaatga
     2461 atacacgccc tagtgtgtgc tgacgagccc gaagctctgg gcgcgcgaga cggcttgcgc
     2521 acgcttttcg acgccaagct ttgtaaagat gtttttgaca tgagatttca ccgtctcggg
     2581 tgcaatgtcg agggtgcgtg caatttcctt gttggactct cccttgccga tgagctctag
     2641 gatgttgcgt tcgcgcccgc ttaatagccc ggtgatgccc aacgtagagc tcgggcgggg
     2701 ccctttctga tagcgctcgc gccaactcga cagaatgtcc tgaagatagg gcaggagttc
     2761 gccgttgctg ttcgtgtgtt gcgcatgttc cagcacgcgc agcaagaggg taccgacttt
     2821 cggccccccg tccagaatca tctggtggat acccgccggc gcggcgacgc tgaccacttc
     2881 gcgaaaagta atcatcgcct gggtcggctc atctgcatcg agcaacgcta cggccagctg
     2941 tatcgacaac cccagcgcgg cgtagtggtc ttgccggccc gcgacttcct cgcgcaattt
     3001 cttcaatatg gcaatgctat ccttcgggcg cttttgcgcc gagacaagct gggcgcgtgc
     3061 catcatggca tagcggcgaa tgtccgacca ggcgcatggg gctgtcgagg gatattcagc
     3121 ggcaaggcga tcaagccggt ccaggcatgc gccgctctcc gtgagccggc cttgcgcgag
     3181 gtgtaaacgc agctgctcgt tcagagcggc ggcgacgagc cgtccccagc tgcgcccgta
     3241 accgagcgtt tccgcctgct caagcagagc gtgtgcgcgc tccaagttcc cgcgccattg
     3301 agcaactcgg accaatacga gatatgcgct caagacgcat tccagcattc cggcgccaga
     3361 aatcagtgga aatcggtcga tgacaagcga ttctgattcg tcaattcggc cttgctcata
     3421 gcgaatccgg gcaaacaagc tggcaggcaa ggcggccgca atcgattgtg gccccatatg
     3481 ttcttcagcc aaacgcatgc catcgaggta gtggtgctcc gcggcgttca ggcggagctg
     3541 ctgagcctcc gcatgaccct gtaggcaacg gcgatagacc gcggcgaaga gattccttcg
     3601 atcgtcatcg gatgaataag gtatccatgg cgtcgcgtag aagctcttga gatcgcccgc
     3661 cttcagatgg ccaaagcggg cgacgttcga ggccacattg ccggtccagg gatcgctcgt
     3721 ccgctcaagg caagctaggg caaggggcag ggatgtctgg ctatcgtcct tcagggcgat
     3781 tgcgaccgat cggatcgctt ggcattcaga tcggaggatc tccgctgccg gcgcgttcgt
     3841 gtcgtctaca tcatcttcga ctcccgctgc cagtgtcaga gcttcctcag agcgcatggc
     3901 gagcgccatt ccccaagcga ttgccaatct gacgtcgagc cgactttgca ttagctcggt
     3961 cggaaacagc cgctgccaac tcagcagcgt gagcaaatcg ccctgcttca ccagcgccat
     4021 cgcgcaattc ttgatccagg cgacagcttg gtcagtgtcg cccgccgcga tcgcgtgatg
     4081 taccgcatcc ctgcatagct cctgggcggc gtaccagcgg tacgcgtgcc gatgcagcca
     4141 ggggatttcg tcgccaagat cgctctgcag cttctggctc agatgtccag ccaggagcgc
     4201 atggtagcga taccaccgcc cctccccgtc gagaggcgca agcagcattt ggcgggcttc
     4261 catggcttcc agcatagcct ggctcgactt gacgcccgtt acggcttgac acagtggggc
     4321 ggagagccgg tcgaggatgg ccgtccgcaa catgaactgg accatgtcgg gcggaaggcc
     4381 ttcgagcatc tcggcgatat aggcgcctat cgggcgcaat gcacctgaca atccgcgcac
     4441 atattgcccg aaatctttgg tcgactgaga ggaggtcgaa gcgataatgc gcagaaccgc
     4501 cggccagcct tccgttttcg catgaagtac ctttacctct tcgggagcta gagtacggag
     4561 attctcctga tcgagaaaat gacgtacctc gtctgggtca aaacgcaggg ccgacacatc
     4621 aatctcgaac aatcggttct gggcgcgcag tacggctaga ggaaggagtg gctcctcgcg
     4681 ggtggtcaac accagatgaa attgggccgg agcatgtttc agcaagaacg atatcgcatc
     4741 atgaatgtcg ggatgcgtta ggcagtggta atcatccaag aacaagtaca tttcgttttc
     4801 gacgtcagcc aggtcgttga tcaaagtaga cacgatcgta ttcagcggcg ccagggaaac
     4861 gtccgaaagt agacggatcg cgctctcgcc cacgctagcg caagcgcgcc gcaacgcctg
     4921 ggagacgtag aagagaaacc ttgtcggctc gtcgtcgtcg gtgtcgagag caagccacgc
     4981 gaccaaatta ccggcttgtt tcagtcgctc cgcccaagag agcgccagcg aggtcttgcc
     5041 gaatcccgcg ccggccttga tcacggttag ctgctttgcg tggagttgat caatcggcct
     5101 gaaaagccgc ggccggtcaa ttagcccagc cgcgtgccgc ggtggcagga tcttggtggc
     5161 gaggagttgt ggctgttggc tggtaatcat tggggtcatg agtagctggt gacagaatct
     5221 cgccgaggaa tcgagcacga cgctgccgtc tgcctccctg cgctccccca aaaacccggt
     5281 ccctcgatcc cccgttcggg ggatgggagc ggttgccgga agagtg
//
LOCUS       DASWGM010000809         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2702522,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000809 DASWGM010000000
VERSION     DASWGM010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2702522"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VGA15_22390"
     CDS             complement(<1..1371)
                     /locus_tag="VGA15_22390"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9590480.1"
                     /translation="MSSALIALEAQVRADLAKTAHPNAPWLKSTPGPDARPALDVLIV
                     GAGQSGIATAFGLMRSQVTNILTIDKAEEGQEGPWLSYARMHTLRSPKHFTGPDLDIP
                     CLTYQSWHEARFGKEHWQNLGLIPRNLWAEYLLWLRRVVGVAVRNGCELLELAPAADG
                     LLAATMKRAGVVETLYARKIVLSTGQEAMGNWTVPEPLRHLPASVLARASEHIDFKAL
                     RGRKVAVIGAGATAFDNAATALEHGAAEVHLLCRRAEIQLVQPYVWLTFRGFLRHFSD
                     LDDAWRWRFMRAILDMREGFPQATYDRCARFANFRLHEGMPVEAAQELGNTVELQTPS
                     GIVAADFAICGTGTYIDFAERSELKNFAGNIATWADRYQPPEDERSARLGRFPYLADD
                     YAFTERVPGQTHWISNIHLFAFASTMSFGPSGSSINAMTTAIPKLVHGLTRGLFRADL
                     ERHWSSF"
     gene            complement(1368..2129)
                     /locus_tag="VGA15_22395"
     CDS             complement(1368..2129)
                     /locus_tag="VGA15_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590481.1"
                     /translation="MRLKGRTAIVTGAGSGIGRATALLFANEGAFVALVDRDTSGLLE
                     TIAIIRDAKGDGSVHVGDVGDAEFAKAVVAELVARHGRLDVLVTAAGFSCGGTVLTTD
                     PADWDAVFRTNVGGTWLWSRAAVPEMQRRGNGSIITLASQLAIAGGRGNSAYIAAKGA
                     IISLTRTMAVDFATDGIRVNAIAPGAIDTPLLRRSFARHADPEPVREVSRNRHAMKRF
                     GKAEEVAETALHLASDASSFTTGTVMVVDGGWLAA"
     gene            complement(2131..3108)
                     /locus_tag="VGA15_22400"
     CDS             complement(2131..3108)
                     /locus_tag="VGA15_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590482.1"
                     /translation="MKILIFGGTGFVGLNIAAALLVRGHAVTLFDRANLPAAAQHDFA
                     GYREALTVVHGDVTDRQAVMDVVADGHDAIVLGAAITAGPARDAADPESIMQVNLLAQ
                     LPVLVAARRSGVVRIINLSSAAAYGAAGSRHALLDEETACDPESLYAITKFASERVAA
                     RLAVLWHLDIVSVRLSGVFGRWERATGVRDTLSPQAQILATIRQQGEAVLPRPGVKDW
                     IYSPDVADAVTLLIEAPKPKHDLYNISTGREWSALQWGQNLAKLHPGFVCRLAESGET
                     PTIDLHGPTDRAPLSVSRLEQEFGWRARFGCAESAVDLSNWWMQHQEGL"
     gene            complement(3188..>3629)
                     /locus_tag="VGA15_22405"
     CDS             complement(3188..>3629)
                     /locus_tag="VGA15_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9590483.1"
                     /translation="NGMPGLQVRLPLLFDAMVSKGRLGLEKFVDLTATAPAKIYNLHP
                     RKGSIAVGADADIAIWDPEREVTLTDAMVHDLAGYTPYAGRKLRGWPVTVLSRGRVIV
                     SDGKRLAEPGSGRFLARSGGDAARPTGRLVADMDPARNFGATLL"
BASE COUNT          719 a         1178 c         1135 g          597 t
ORIGIN      
        1 gaaagaactc cagtgccgct caaggtccgc gcggaacaat ccgcgggtca ggccatgcac
       61 cagcttgggt atggcggtag tcatcgcgtt gatcgatgag ccggatggac cgaaactcat
      121 ggtggaagca aatgcaaaca ggtgaatatt ggaaatccag tgcgtctgtc ccggcacgcg
      181 ttcggtgaag gcgtaatcat cggcgagata ggggaaacgg cccagccgcg cgctgcgctc
      241 atcttccggc ggctggtaac ggtcggccca ggtcgcgatg ttgccggcaa agtttttgag
      301 ttcgcttcgc tcggcaaaat cgatgtaggt gccagtgcca cagatggcga agtccgccgc
      361 gacgatgccg cttggcgtct gtaattcgac cgtgttgccc agctcctgag cggcctcgac
      421 cggcataccc tcatgcaggc ggaaattggc gaagcgcgcg cagcggtcgt aggtcgcctg
      481 cggaaatccc tcccgcatgt cgaggatggc gcgcatgaaa cgccagcgcc aggcgtcgtc
      541 cagatcgcta aaatgccgca gaaaaccgcg gaaggtcagc caaacatagg gctgcaccag
      601 ctgaatttct gcgcggcggc aaagcagatg cacttcggcc gcgccgtgct caagcgccgt
      661 cgccgcgtta tcgaaggcag tcgcaccggc gccgatcacg gccactttcc tgccacgcag
      721 tgccttgaag tcgatgtgtt ccgatgcgcg cgccaatacg ctggccggca gatggcgcag
      781 gggctccggg acggtccagt tgcccatggc ttcctgaccg gtcgacaaca cgatcttgcg
      841 cgcgtaaagc gtttcaacga cacccgcacg cttcatcgtg gcggcgagca atccatccgc
      901 cgcgggtgca agctccaata gctcgcagcc gttgcgcact gccacgccga caacgcgccg
      961 cagccagagt agatactcag cccaaaggtt gcgcgggatc agaccaagat tctgccaatg
     1021 ttctttgccg aagcgcgctt cgtgccagga ctggtaggtg agacagggaa tgtcgagatc
     1081 gggcccggtg aaatgcttcg ggctgcgcag cgtatgcatg cgggcatagg aaagccacgg
     1141 gccctcctgc ccctcctcgg ccttgtcgat ggtgaggatg ttggtgacct gggagcgcat
     1201 gagcccgaat gcggtagcga taccggattg accggcgccg acgatgagca cgtcgagcgc
     1261 cggcctggca tccgggcccg gcgtcgactt gagccagggc gcgttcgggt gcgccgtctt
     1321 ggcaagatcg gctcggacct gagcctcgag ggcgatcaga gcgctgctca tgccgcaagc
     1381 caaccgccat ccacgaccat gacggtcccg gtcgtgaacg acgatgcatc gctggcaagg
     1441 tgcagcgcgg tctcggcgac ttcctcggcc ttgccgaacc gcttcatggc atggcggttg
     1501 cgtgacactt cacgaaccgg ctccggatcg gcatgacgag cgaaactgcg ccggagtaac
     1561 ggcgtgtcga tcgcaccagg cgcaatggca ttgacgcgaa tgccgtcggt cgcaaaatcc
     1621 accgccatcg tccgggtcag gctgatgatt gcgcccttgg cggcgatata ggcgctgttg
     1681 cccctgccgc cggcgatcgc aagttgcgag gcaagcgtga taatcgagcc gttgccccgc
     1741 cgctgcatct ccggcacggc cgcacgcgac caaagccagg tgccgccaac attggtgcgg
     1801 aagaccgcgt cccaatcggc gggatcagtc gtcagcaccg tgccgccaca agaaaacccg
     1861 gcggcggtca ccagcacatc gagccggccg tggcgcgcca caagctcggc gaccacggct
     1921 tttgcgaatt cagcgtcacc cacatcgccc acatgcaccg atccgtcgcc cttggcgtcg
     1981 cgaatgattg cgatcgtttc cagtagtccg gaagtatcgc gatcgaccag cgcgacaaac
     2041 gcgccttcat tcgcgaacaa cagcgccgtg gcgcgaccga tgccggaacc ggccccggtg
     2101 acgatcgcgg tccgcccctt caatcgcatg tcaaagccct tcctgatgct gcatccacca
     2161 attgctcaga tcgaccgcag actcggcaca gccaaaacgg gcgcgccagc caaactcctg
     2221 ctccagacgc gaaaccgaca gcggcgcccg gtcggtcggg ccgtgaagat cgatggttgg
     2281 cgtctcgcca gattcggcga gacggcagac aaaaccggga tgcagcttgg ccagattctg
     2341 tccccattgc agcgccgacc attctcgccc ggtcgaaatg ttgtaaagat catgtttcgg
     2401 cttcggcgct tcgatcagca acgtcacggc gtccgcgaca tccggggagt agatccagtc
     2461 cttgacgccc ggacgcggca gaacagcttc gccttgctga cgaatggtgg caaggatctg
     2521 cgcttgtgga ctgagcgtgt cgcgtacccc ggtcgccctt tcccatctcc cgaacacgcc
     2581 gctcagccgc acactgacga tatcgaggtg ccagagcaca gccagacgcg cggcaactct
     2641 ctcggaagca aatttggtga tggcatagag cgactcggga tcgcaggccg tctcttcatc
     2701 gagcagggcg tgccgcgagc cggcagcgcc ataggccgcg gcggatgaca gattgatgat
     2761 ccgcacgacg ccgctgcgcc gcgctgcgac cagaaccggc aactgagcca gcagattgac
     2821 ctgcatgatc gattcagggt ccgccgcatc gcgcgcgggg ccggcggtga tcgctgcccc
     2881 gagcacgatc gcatcatggc cgtcagcgac aacgtccatg acagcttgcc gatcggtgac
     2941 gtcgccgtga acgacagtca gcgcttcccg atagccggcg aaatcgtgtt gcgcggcggc
     3001 gggcaggttc gcgcgatcga acaaggtgac ggcgtgcccg cgcaccagca gggcggcggc
     3061 gatattgagc ccgacgaaac cggtgccgcc gaagatcagg attttcatgt cccagcccca
     3121 gcacctgtcg cgatcagcag cgacgaccat cgccatcgat agatccggcg acagtacgat
     3181 aaaatgctca cagcagggtc gcaccgaaat tccgcgccgg atccatatcg gcgacaagtc
     3241 ggccagtcgg cctcgctgca tccccgccgg aacgcgcgag gaagcgaccg gaaccaggct
     3301 cggccaaacg cttgccatcc gagacaatta cgcgtccgcg cgacagcacc gtgaccggcc
     3361 agccgcgcag cttgcggcca gcatagggcg tatagccggc aaggtcgtgc accatggcgt
     3421 cggtcagcgt gacctcgcgc tccgggtccc agatcgcgat atcggcatcg gcgccgacag
     3481 cgatagagcc cttgcgcgga tgcaggttgt agatcttggc gggcgcggtc gccgtcaaat
     3541 cgacgaattt ttccagcccc aggcgaccct ttgaaaccat cgcatcaaac agcagcggca
     3601 ggcgcacctg cagtcccggc ataccgttc
//
LOCUS       DASWGM010000810         4366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1595239,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000810 DASWGM010000000
VERSION     DASWGM010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595239"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VGA15_22410"
     CDS             complement(<1..258)
                     /locus_tag="VGA15_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX9590484.1"
                     /translation="MDSKIDTAPIELLEDLLASRYSCRAFRPGPVPRLTIERILTAAQ
                     KTASWCNSQPWQLAIASGAATQKFRDAIHGAASSGKPNTGDF"
     gene            complement(449..1693)
                     /locus_tag="VGA15_22415"
     CDS             complement(449..1693)
                     /locus_tag="VGA15_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9590485.1"
                     /translation="MNFDDAPQEAEFRATARQWIAANAPKQFEAELSKSSLGRIRLEK
                     EEIVDVGKAWQKKKADAGWACLHWPKEYGGRGATPIERVIWQQEEGVYGKLTQPFQIG
                     EGMCGPTVMAYGSEEHKRHYLPKLAAGEQIWCQLFSEPAGGSDVAGLRTRAERRGDDW
                     IVNGQKIWTSGAHYSDFGLLITRTDPNVPKHKGLTMFFLDMKNPGVEVRPIKQANGMQ
                     EFNEVYFTDVVIPDHQRLGAVGDGWNVSLTTLMNERMSIGSRLATGFPEMFDFCSSLM
                     TDDGLAIDDRATRSKLANWAVKASGLKYTSYRAISALSKGERPGPENSIGKLVAGSML
                     QDIATYAMDLEGAAGVFTGAAEEEARGQFQQMLLNSPSMRIAGGTDEILRNIIAERVL
                     GLPGDIRVDKNVPFNKIPTKGR"
     gene            complement(1844..3088)
                     /locus_tag="VGA15_22420"
     CDS             complement(1844..3088)
                     /locus_tag="VGA15_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9590486.1"
                     /translation="MNFDDTPQEAAFRAEAKTWISANAPKQYEDELKKSSLGRTQLKG
                     ANILEVAKAWQKKKADAGWACLHWPREYGGRGATPIERVIWQQEEGAFGKLSGMFIIG
                     HGMCGPTMMAFAGEDQKRKYLPPLASGEKVWCQLFSEPAGGSDVAGLRTRAERRGDDW
                     IINGQKIWTSGAHYSDYGILLTRTDPNVAKHKGLTMFFLDMKSEGVEIKPIKQANGQS
                     DFNEVYFTDVKIPDVQRLGEVNDGWNVSLTTLMNERMSIGAGVSTGFPELFEFCNSLM
                     LEDGPAIDDRNVRSKLANWAVRASGLKYTSMRAISALSKGERPGPENSIGKLVAGSMI
                     QDVAAYALDLQGAAGVLSGPEDAEASGKFQAMLLRAPGTRVEGGTDEIMRNIIAERVL
                     GLPGDIRVDKDVPFNKIPTKGR"
     gene            complement(3274..>4366)
                     /locus_tag="VGA15_22425"
     CDS             complement(3274..>4366)
                     /locus_tag="VGA15_22425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9590487.1"
                     /translation="RDEARKFLAEKCPPKAVRVVLDGKEPYDRELWKGLAEMGFLGVA
                     IPEEFGGAGAGHLELCVIAEEMGRALAPVPFSSTVYLAAEAVLLAGSDAQKRKWLPLI
                     ASGDAIGTLALFEGKGSLSPAAIKLLAPGGTLNGVKKPVPDGAIADFAIVAARTGSTG
                     RDSDISLFLVEMKAEGVEARALANIDPSRGQAELIFRNCKAEPLGAANEGWGILSEVL
                     DRAAVLVAFEQVGGADRALEMGRDYALDRIAFGRPIGSFQAVKHMLADMYVSATLARS
                     NCYYGAWALSTNASELPEAAAAARISATQAFQHCAKNNIQVHGGMGFTWEFDCHMYYR
                     RANATALGLGSLSYWEDALIDRMRKRNAA"
BASE COUNT          722 a         1451 c         1319 g          874 t
ORIGIN      
        1 aaagtcgccg gtattcggct tgccgctgga cgcggcgcca tggatcgcat cgcgaaattt
       61 ttgggtcgcc gcacccgagg cgatggcgag ctgccagggc tggctgttgc accacgacgc
      121 ggttttttgc gcagccgtca atatgcgctc gatggtcaag cgcggcaccg ggccgggccg
      181 gaacgcgcgg caggaatagc gcgatgccag cagatcttcg agaagctcga tcggcgcggt
      241 gtcgattttg ctgtccatca cgtcatcgcc tctgttggag ccgtgccccg gacgcagcgc
      301 agcgccataa gcgtgtttac gcgcgtcttc gacgcgctat ggcggtgcgc tgcagagccg
      361 gggtccatac tttccttact ggtcgttgtg ggtcccggct ctgcggagca gcgttgcacg
      421 ctgcaccgcg tccgggacac gagagttttc atcgtccttt ggtcgggatc ttgttgaacg
      481 gcacattctt gtcgacgcgg atgtcgccgg gcagccccag cacgcgctcg gcgatgatgt
      541 tgcgcaggat ttcatcggtg ccgccggcga tgcgcatcga cggcgagttc agcagcatct
      601 gctggaactg tccgcgcgcc tcctcttccg cggcaccggt aaagacgccg gccgcacctt
      661 cgaggtccat cgcataggtc gcgatgtcct gcagcatcga gcccgccacc agcttgccga
      721 tcgagttttc cggccccgga cgttcgccct tggatagcgc cgatatcgcc cgatagctgg
      781 tgtatttcag cccgctcgct ttcaccgccc agttggcgag cttcgagcgt gtggcgcgat
      841 cgtcgatggc gagcccgtcg tccgtcatca gactggagca aaaatcgaac atctcgggaa
      901 agccggtcgc aagacgggag ccgatcgaca tccgctcgtt catcagcgtg gtcagcgaca
      961 cgttccagcc gtcgccgacc gcaccgaggc gctgatgatc gggaatgacg acgtcggtaa
     1021 aatagacctc attgaactcc tgcatgccgt tggcctgctt gatcggcctc acttcgacgc
     1081 cggggttctt catatcgaga aagaacatcg tcaatccctt gtgcttgggc acattgggat
     1141 cggtccgggt gatcagcaga ccgaagtcag aataatgcgc gcccgaggtc cagatcttct
     1201 ggccgttgac gatccagtcg tcgccgcgtc gctccgcgcg ggtgcgcagg cccgcgacgt
     1261 cagacccgcc ggccggttcc gaaaacaact ggcaccagat ctgttcgccg gcggcaagct
     1321 tcggcaggta atggcgcttg tgctcctcgg agccataggc catcacggtc ggcccgcaca
     1381 tgccctcgcc gatctggaac ggctgcgtca gcttgccgta cacgccttct tcctgctgcc
     1441 agatcacgcg ctcgatcggc gtggcgccgc gcccgccata ttccttcggc cagtgcagac
     1501 aggcccagcc ggcgtcggcc ttcttcttct gccaggcctt gccgacatcg acgatttctt
     1561 ctttctccag cctgatccgg ccgagcgagg attttgacag ctccgcctcg aattgtttcg
     1621 gcgcgttggc ggcgatccat tggcgggccg tggcgcggaa ttcggcttcc tgcggggcgt
     1681 catcgaaatt cattcggctt ctccttaact ctggaacgtt ccccggatgc tgcgcagcgc
     1741 gaagcggtgc gctgctgatc cgaggcccat cgattgactc tcttgtgggt ctcggctctg
     1801 cggagcagcg ttgcacgctg caccgcgtcc gggacacgat gatctacctt cccttggtcg
     1861 gtatcttgtt gaacggcacg tccttgtcga cccggatatc gccgggcagg ccaagcacgc
     1921 gctcggcgat gatgttgcgc atgatctcgt cggtgccgcc ttcgacgcgg gtgccggggg
     1981 cgcgcagcag catcgcctgg aattttccgc tggcttccgc gtcctcgggg ccgctcagca
     2041 cgccggccgc gccctgcaaa tccagcgcat aggccgcgac atcctgaatc atcgaccccg
     2101 ccaccagctt gccgatcgaa ttctccggtc cgggacgctc gcccttcgac agcgccgaga
     2161 tcgcgcgcat gctggtgtat ttcagcccgc tggcgcgcac cgcccagttg gcgagtttcg
     2221 agcgaacatt acgatcgtcg atcgcggggc catcttccag catcaggctg ttgcagaatt
     2281 cgaacagctc cggaaagccg gtcgaaacac cggcgccgat cgacatgcgc tcgttcatca
     2341 gtgtggtcag tgacacattc cagccgtcat tgacttcgcc gagccgctgc acatccggaa
     2401 ttttcacgtc ggtgaaatag acttcattga aatccgactg accgttggcc tgcttgatcg
     2461 gcttgatctc gacaccttcg ctcttcatgt ccaggaagaa catggtgagg cccttgtgct
     2521 ttgccacatt cggatcggtg cgggtgagca ggatgccgta gtccgaataa tgcgcgccgg
     2581 aagtccagat cttctggccg ttgatgatcc agtcgtcgcc gcgtcgctcc gcgcgcgtgc
     2641 gcaggccggc gacgtcggaa ccgcctgccg gttcggagaa caactggcac cacacctttt
     2701 cgcctgacgc cagcggcggc aggtattttc gcttctggtc ctcgccggca aacgccatca
     2761 tggtcggccc gcacatgccg tggccgatga tgaacattcc gctgagcttg ccgaacgcgc
     2821 cctcctcctg ctgccagatc acccgctcga tcggggtcgc gccacgcccg ccatattccc
     2881 ttggccagtg caggcaggcc cagccggcat ccgccttttt cttctgccag gctttcgcca
     2941 cctcaaggat gttggcgccc ttgagctggg tgcggccgag cgaggatttt ttcagttcgt
     3001 cctcgtactg cttcggcgca ttggccgata tccaggtctt ggcctcggcg cggaacgcag
     3061 cctcctgcgg ggtgtcatca aagttcatga cggttctctc tcatctctcg acacgttccc
     3121 cggacgctgc gcagcgccat tagcgcgttc acgcgagtct tcgacacgct atggcggtgc
     3181 gcagcagatc cggggtccat caactggccc tatgggtccc ggctctgcga agcagcgcga
     3241 agggcgctgc attgcgtccg ggacacgaga acctcaagcc gcattccttt tccgcatccg
     3301 gtcgatcagc gcgtcttccc aataggaaag actgccaagc ccaagcgccg tcgcattggc
     3361 gcgccgataa tacatgtggc aatcgaactc ccaggtaaaa cccatgccgc catgcacctg
     3421 gatgttgttc ttggcgcaat gctggaacgc ctgcgtcgcg ctgatgcgcg cggcggcggc
     3481 ggcttccggc agttccgacg cgttggtcga gagcgcccag gcgccatagt agcaattcga
     3541 ccgcgccagc gtcgccgaaa cgtacatgtc ggcgagcatg tgcttcaccg cctggaacga
     3601 gccgatcggc cggccgaagg cgatacgatc cagcgcgtag tcgcgcccca tttccagcgc
     3661 gcggtcggcg ccgccgacct gctcgaacgc caccagcacc gcggcgcggt cgagcacctc
     3721 tgagagaatg ccccagcctt cattggcggc accgagcggc tcggccttgc aattcctgaa
     3781 gatcaattcg gcctggccgc gcgacggatc gatattggcc agggccctgg cttcgacgcc
     3841 ttcggctttc atctcgacca agaacagcga gatatcgctg tcgcgtccgg tcgagccggt
     3901 gcgcgcggca acaatggcga agtcggcaat cgcgccgtcg ggcaccggtt tcttgacgcc
     3961 gttgagcgtg ccgccgggcg ccaacagttt tatcgctgcc ggcgacagac tgcccttgcc
     4021 ttcgaacagc gccagcgtgc cgatcgcgtc ccccgatgca atcaagggca gccacttccg
     4081 cttttgcgca tcgctgcccg cgagcaggac ggcttccgca gccagataga cggtcgatga
     4141 aaacggcacc ggcgccagcg cgcggcccat ttcttccgcg atcacgcaga gttcgagatg
     4201 gccagcaccg gcgccgccga attcctccgg gatcgcgacg ccgagaaagc ccatctcggc
     4261 aagacccttc cacaactcgc ggtcatacgg ctccttgccg tcgagcacga cgcgcaccgc
     4321 cttgggcggg cacttttcgg caagaaattt tcgcgcttcg tcgcgg
//
LOCUS       DASWGM010000811         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_367646,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000811 DASWGM010000000
VERSION     DASWGM010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367646"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGA15_22430"
     CDS             complement(<1..270)
                     /locus_tag="VGA15_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9590488.1"
                     /translation="MAEIKKVLDVAGVSKRFGDVEVLSDVSFQVDKGETICVLGPSGS
                     GKSTLLRCINWLERPDAGQIYLNGERVGINGAGIIMSDRDLSQIRT"
     gene            complement(280..936)
                     /locus_tag="VGA15_22435"
     CDS             complement(280..936)
                     /locus_tag="VGA15_22435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667434.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9590489.1"
                     /translation="MIHDFGIVWNERALLLSGLANTAILSTLAAIAALLLGFILTPAL
                     MSKQRAVCSAARVFVDGMRCVPFLLFAYIVYYGFPSFGVRLDNWTSGLAALTIYNAAY
                     MAEILRGAWAVQPREPIEAGIAFGFSEIKLFRRIILPPLLLSAGPVIGNQMIQIIKDS
                     AFLTIIALPELTHAASSIQSRHYVPFAAFITAVFLYWGLCLVIEAGVSSIGRVAEARR
                     "
     gene            complement(933..>1562)
                     /locus_tag="VGA15_22440"
     CDS             complement(933..>1562)
                     /locus_tag="VGA15_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9590490.1"
                     /translation="AAIFQGAIVTVTLSLVGILIGLPIGLGLALIRWANVPIAAGFVT
                     LYVSMLRATPLVTLLLLLFFALPNIGVPINPISAAIVALVMNTSAFNCEVWRSALMSF
                     PRDQYEAAQSVGMRAGQRFRRIVLPQIVRVSLPGLVNEMSLLIKVTPVLAVVGVVDIT
                     RAAVRIGAETYEPLPPFLVAIALYAPIVFALVSLQRWIERRQVAAEAVA"
BASE COUNT          363 a          491 c          459 g          249 t
ORIGIN      
        1 ggttctgatc tgcgacaaat cgcggtcgct catgatgatg ccggcaccat tgatgccgac
       61 ccgctcgccg ttcaagtaga tttgcccggc gtcggggcgt tcaagccagt tgatacagcg
      121 caacaatgtc gatttgcccg acccggacgg tccaagcacg cagatcgtct cacccttgtc
      181 gacctggaag ctgacgtcgg agaggacctc gacatcgcca aaccgcttcg acaccccggc
      241 gacatcgaga acctttttga tttctgccat cgctcgtccc tatcgacggg cttcggcaac
      301 gcggccgatc gaactgaccc cggcctcgat cacgagacag agtccccaat acagaaacac
      361 cgcggtaatg aaggccgcga acggaacgta atgccgcgac tggatcgagc tcgcggcgtg
      421 cgtcagttcc ggcagtgcga tgatggtcag gaaggcgctg tccttgatga tctggatcat
      481 ctgattgccg atcaccggcc ccgccgataa cagcagcggc ggaaggatga tgcggcgaaa
      541 cagcttgatc tccgaaaagc cgaacgcgat gcccgcctcg atcggttcgc gcggttgcac
      601 cgcccaggcg ccgcgcagaa tctccgccat ataggcggca ttatagatgg tcagggcggc
      661 cagtcccgag gtccagttgt ccagcctgac gccgaacgac ggaaagccat aatacacaat
      721 gtaggcaaac agcagaaacg ggacacatcg cattccgtcg acaaacacgc gggccgcgct
      781 gcaaacggcc cgttgtttcg acatcagcgc gggggtcagg atgaacccca acaaaagtgc
      841 tgcgatagcg gcgagcgtgg acaatatcgc cgtattggcg agcccgctca gaagcaatgc
      901 gcgttcgttc cagacaatac cgaaatcgtg gatcatgcga cggcctcggc cgcgacctgg
      961 cgccgttcga tccagcgctg cagcgacacc agcgcaaaga cgatcggcgc atacagcgct
     1021 atcgccacca gaaatggcgg cagcggctca taggtctcgg cgccaatccg cacggcggcg
     1081 cgggtgatgt cgacgacccc gacgaccgca agcaccggcg tgaccttgat cagcagcgac
     1141 atctcattga cgagaccggg aaggctgacg cgcacgatct gcggcaagac gatcctgcga
     1201 aaccgttgcc cggcgcgcat gccgaccgac tgcgccgcct cgtactgatc ccttggaaaa
     1261 ctcatcagtg cggaccgcca tacctcgcag ttgaaggccg aagtgttcat gaccagcgct
     1321 acgatcgctg ccgagatcgg attgatcggc acgccgatat tgggaagggc gaaaaacaac
     1381 agcagcaaca gtgtgaccag cggggtggcg cgcaacatac tgacgtaaag cgtcacgaac
     1441 cctgccgcga ttggaacatt cgcccaccgg atcaaggcaa ggccaagtcc aatcggcagc
     1501 ccgatcagga tgccgacaag cgacagcgtc actgtcacga tggcaccctg aaagatggcc
     1561 gc
//
LOCUS       DASWGM010000812         8105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_497116,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000812 DASWGM010000000
VERSION     DASWGM010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497116"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VGA15_22445"
     CDS             <1..268
                     /locus_tag="VGA15_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEX9590491.1"
                     /translation="SDAGALLEAEQGAIEHMNADHRDAMNLYATRLLGAEPADWCCTG
                     CDPDGIDMQAGPTTLRLDFPERVTGPGELRKMLVRLAGEARAKG"
     gene            271..648
                     /locus_tag="VGA15_22450"
                     /pseudo
     CDS             271..648
                     /locus_tag="VGA15_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
     gene            966..2585
                     /locus_tag="VGA15_22455"
     CDS             966..2585
                     /locus_tag="VGA15_22455"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX9590492.1"
                     /translation="MQEETGVRNGAFGADKFGLKNLKGVNWNFGAPQLYEHSLATGEA
                     VLSTDGALCADTGVFTGRSPKDKFTVRDAVTDKNMWWAGNQSITSEQFEALYADFLGH
                     AEGMTLFAQDLYGGADPALRIKTRVFTELAWHSLFIRTLLIRPETSELASFVPELTII
                     DLPSFRADPKRHGVRSENVVAIDFARKIVLIGGSYYAGEMKKSVFTTLNYYLPEKGVM
                     PMHCSANVGPDGDAAVFFGLSGTGKTTLSADPKRTLIGDDEHGWGGDGIFNFEGGCYA
                     KCIKLSKEAEPEIYAASKRFGAVLENVVLDARTREPDFDDGSKTENTRSAYPLEFIPN
                     ASRTGRAGHPKNVVMLAADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTERGLGKEP
                     QPEFSTCFGSPFLPRDPSVYGNLLRDLIAKHDVDCWLVNTGWTGGKYGTGRRMPIKVT
                     RALLTAALNGSLRNADFRTDRYFGFAVPTSLPGVEPHILDPIKTWKDKAEFDQTARAL
                     VGMFQKNFAKFEAQVDAEVRAAAPEVKLAAE"
     gene            complement(2698..4281)
                     /locus_tag="VGA15_22460"
     CDS             complement(2698..4281)
                     /locus_tag="VGA15_22460"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9590493.1"
                     /translation="MRGISTSGDLVVRLFDGPLMGINHARSAHAALNGARLLMQAGLS
                     AGAPKETVEKIEKLVAGITDDLKVVRDRVKTKNVIAALEQAETRLRDWSDTGLKILKP
                     QAGGLTALPTPFSVAKKGDAAAAAVDDLVEMVAAYGFDYRTEAEATVAADHAAMLALA
                     VATALVGLALAVAFAHSMSKPIFAAMQIAEHVAAGNFTDRIELRRRDELGRLLKSLAV
                     MQAGLKARAEEDSALMSSKDQANSEQVSRRTRMEGEIEAFRSTITSVLTNADAMTGEL
                     AETARTLSSIAQVAGQQSIETASSADETSANVQTVSAAAGLLGESVQAIEGQLHEATS
                     IVRRASSMAEDANQTMGALATAAQHIDDVVGFIRNIAGQTNLLALNATIEAARAGDAG
                     RGFAVVASEVKALAIQTAKATEEISSQIAEVQLATKRAVDNVGAITAVMGEIDSFTAT
                     IATAVNQQNTAAAEITKNIRQAAAGTASVARGIAGTAAASEDTNRSADMVLSSAHGLS
                     RQAAELRSSVDRFLSNVAA"
     gene            complement(4386..4706)
                     /locus_tag="VGA15_22465"
     CDS             complement(4386..4706)
                     /locus_tag="VGA15_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9590494.1"
                     /translation="MRKLASLTFVVLAGLSVGALAANLTITQKGRVFSSETVTIKKGE
                     TLTFANDDSVPHNIISTSKGNEFNLGSQPPGAATDVTFKESGEALVICAIHPRMKMTV
                     KITD"
     gene            complement(4737..5822)
                     /locus_tag="VGA15_22470"
     CDS             complement(4737..5822)
                     /locus_tag="VGA15_22470"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX9590495.1"
                     /translation="MLPSEFIRSVRSGPVVAAVALGLIGFTNLGFGAAIVGEMRRTEP
                     SPKAAVALDAMKAEYRRPALIPFPKENPYTPDKASLGKKLYFDTRISVTSAQSCASCH
                     SPGFGWGDGLAVGVGHGMAKLGRHSPTIVNAAWGGIFMWDGRLANLEEQALGPIQSPG
                     EMNMPIEQLMERLATIAEYQPLFAAAFPGAGLSPKSLAAAIATYERTVVSEEAPFDLW
                     IEGNEQAISDEAKRGFVLFNTKAQCSSCHEGWNFTNDGFQDIGLPSADVGRGQFLPQV
                     IKMKHAFKTPGLREIARRGPYMHDGSIATLEQVIEHYDQGGVDRPSRSDLVKPLNLTP
                     QEKSELVAFLKTLTSNLDPTTVPVLPR"
     gene            complement(5982..6962)
                     /gene="rocF"
                     /locus_tag="VGA15_22475"
     CDS             complement(5982..6962)
                     /gene="rocF"
                     /locus_tag="VGA15_22475"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEX9590496.1"
                     /translation="MSGTSKSPGPPQIALLGVPIEIGASQRGTLMGPDALRTAGIGRV
                     LEQLGFDVEDHGNLPVHAVSSADGPPPVNAKYYDDIKGWIRALSERAFLLARSGAIPI
                     FMGGDHSLSMGSVNGVARYWQELGRPVFVLWLDAHADYNTPATTVTGNMHGMSAAFLC
                     GEPGLDGLLGDGARVSISPDQLDLFGIRSIDPLEKDLVRDRRIAVADMRAIDEFGVGV
                     LIRRVIERVKARNGVLHVSFDVDFLDPAVAPGVGTTVPGGATYREAHLVMELLHDSGL
                     VRSVDIVELNPFLDERGRTARVAVELLGSLFGLQITDRPTPSNAVLPDGT"
     gene            complement(7011..7829)
                     /locus_tag="VGA15_22480"
     CDS             complement(7011..7829)
                     /locus_tag="VGA15_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9590497.1"
                     /translation="MLTKTQHLFDDATRVTAGDSRWQGRTSDDYWAFVGPFGGITAAT
                     ILRALIDHPQRSGDPLSVTVNCCAPIAQGAFDLDVRLIKANRSTQHWRVELSQGGAEV
                     ATLATAVFAERRPSWSHQRAEFPKSPPFAQIQPYPKVAAPWVKQYDFRFVEGHPDFSG
                     AVKDAPANAFSKLWIGDRVPRKIDMLSLMSMSDAFFGRVFHARRELVPFGTVSLTTYF
                     HVDAEDLAAEDIDRVLAVADAKIFHKSYGDQAGELWSPQGRLLATTHQTAYFKA"
BASE COUNT         1524 a         2476 c         2560 g         1545 t
ORIGIN      
        1 gtcggacgcc ggagcgctgc tggaagccga gcagggcgcc atcgagcaca tgaacgccga
       61 tcatcgcgat gcgatgaacc tctatgcgac caggctgctc ggcgccgagc ctgcggactg
      121 gtgctgcacc ggctgcgatc ccgatggcat cgacatgcag gccggcccaa ccacgctgcg
      181 gctggatttc ccggagcggg tgaccgggcc gggagagttg aggaaaatgc tggtgaggct
      241 ggcgggcgaa gcgcgcgcga agggatagcg ccgtccgagc tcgacgacgg tctgctcgcc
      301 gccagcgaag tcgcgccgtt gaggctcaat gccaattggg tggtgctgtc ggcctgcaac
      361 accatcgccg gcgacaagcc cggcgcggag gcactgtcgg gtctcgcacg gtcgttcttc
      421 tacgcggtcg cccgggcgct gctggtctcg cacggggccg tcgattcggc ggccgcaacc
      481 cggctcactg tcgcgacctt cgatcgcctc aaatccgatc caaagctcgg ccgcgccgag
      541 gccctgcgtc aggcgatgct cggctatctc aacgatgcgt cgtcgccccg caacgcctat
      601 ccagcggtct ggggtccgtt tgcgctgatc ggcgaggggg cggctcgaca agtcgggagt
      661 gtgcaccgca gcattaaggt tacggacgca aatttacgca gattgcgact cttggtagcg
      721 ctttggatgg cgagggcttc tcgcgcaacg tgccgatcag gtccgcgctg cagcaagcgt
      781 tgagccgggt caatgactcg cctgggaata aaccaaatac tgagggatcg gcttggcaac
      841 cgcggcgttc atcagtatta gggtcgtcgg accgaagccg gaaggctata ttgacggtca
      901 ccgcgagagg cgcgtctggc ggcgtcggcg cgttcgagtt acgcgggttc tgggaggatt
      961 attcggtgca agaagagacg ggcgtacgca acggtgcctt cggtgccgat aaatttggtt
     1021 taaaaaatct caagggcgtg aactggaatt ttggcgcgcc gcaactttac gaacactccc
     1081 tggctaccgg tgaagccgtg ctttccaccg atggcgccct gtgcgcggac accggtgtgt
     1141 tcacgggccg cagccccaag gacaaattca ccgttcgcga cgccgtgacc gacaagaaca
     1201 tgtggtgggc cggcaaccag tcgatcacgt cggaacaatt cgaagcgctc tacgccgact
     1261 tcctcgggca tgccgaaggc atgacgctgt tcgcacagga tctctatggc ggcgccgatc
     1321 cagccttgcg gatcaagacc cgtgtcttta ccgaactcgc ctggcattcg ctgttcatcc
     1381 gcacgctgtt gattcgcccg gagacatccg agctcgcgag ctttgtgccg gaactcacta
     1441 tcatcgatct gccgagcttc cgcgccgatc cgaaacgcca cggcgtgcgc tcggagaacg
     1501 tggttgcgat cgatttcgcc cgcaagatcg tcctgatcgg cgggtcttat tatgccggcg
     1561 agatgaagaa gtcggtattc accacgctga attattacct gcccgaaaaa ggcgtcatgc
     1621 cgatgcactg ctcggccaat gtcggccccg acggcgatgc tgcggtcttc ttcggtctgt
     1681 ccggcacggg gaaaaccacg ctctcggccg atcccaagcg cacgctgatc ggcgacgacg
     1741 agcacggctg gggcggcgac ggaatcttca atttcgaagg cggctgctat gccaagtgca
     1801 tcaagctgtc gaaagaagcc gagcccgaaa tttatgccgc cagcaagcgt tttggcgcgg
     1861 tgctggaaaa cgtcgtgctc gacgcgcgca cccgtgaacc ggatttcgac gatggctcga
     1921 aaaccgaaaa tacccgttcg gcctatccgc tcgaattcat ccccaacgcc tcgcgcaccg
     1981 gccgtgccgg tcatccgaag aatgtggtga tgctggccgc cgatgcgttt ggcgtgatgc
     2041 cgccgatcgc gaagttgacg ccggcgcagg cgatgtatca cttcctgtcc ggctacaccg
     2101 cgaaggtggc gggcaccgag cgcgggctcg gcaaggagcc gcagccggag ttctcgacct
     2161 gcttcggttc gccgttcctg ccgcgcgacc cttccgtgta cggcaatctg ttgcgcgact
     2221 tgattgcaaa gcacgatgtc gattgctggc tggtcaacac cggctggacc ggtggcaaat
     2281 acggtaccgg ccgccgcatg ccgatcaagg tcacgcgggc gctgttgacc gcggcgctga
     2341 acggctcgct gcgcaatgcc gatttccgca ccgacagata tttcggcttc gcggtgccga
     2401 cctcgctgcc gggcgtcgaa ccgcacatcc tcgatccgat caagacctgg aaggacaagg
     2461 ccgagttcga ccagaccgcg cgcgcgctgg tcggcatgtt ccagaagaat ttcgcgaagt
     2521 tcgaagccca ggtcgacgcc gaggtccgcg ccgcggcacc tgaagtgaag ctcgcggcgg
     2581 agtgacccgc aggatcattc cggggcgcgc tagcgcaaac ccggaatctc tggattccgg
     2641 atcgctccgc agatggagcg tccggaaccg gcggtcgcga cgccgccttt ttcattgcta
     2701 agccgccacg ttggacagga agcgatcaac cgacgagcgc agttccgcgg cctggcggga
     2761 caggccgtgt gcagagctga gcaccatatc tgccgagcgg ttggtatcct cgcttgccgc
     2821 ggcagttccc gctatgcccc gtgcgacgct tgccgttccg gcggcagcct gacggatatt
     2881 cttggttatt tcagccgccg ccgtattctg ctggttcacg gcggtggcga tcgtcgcagt
     2941 gaagctgtcg atctctccca tgacggccgt gatggctccg acgttgtcca ccgcccgctt
     3001 tgtcgccagc tgcacctcgg cgatttgaga ggagatttcc tcggtggcct ttgcggtctg
     3061 gatcgccagc gccttcactt ccgaagcaac cactgcgaag ccgcggccgg catcgccggc
     3121 ccgcgccgcc tcgatggtgg cgttgagggc cagcaggttg gtctggccgg cgatgtttcg
     3181 gatgaaaccc accacgtcat caatatgctg ggcagcggtc gcgagcgctc ccattgtctg
     3241 gttggcgtcc tcagccatgc ttgaggcgcg ccgtacgatg ctggtggctt cgtggagttg
     3301 tccctcgatg gcctgcacgg attctccaag caggcccgcc gcggccgaga cggtctggac
     3361 gttggcggac gtctcatcgg cgctcgatgc cgtctcaatc gattgctgac cggccacctg
     3421 ggcaattgac gacagagttc gcgcggtctc ggccaactcg ccggtcattg cgtcagcgtt
     3481 ggtaagcacg gaagtgatgg tggaacggaa tgcctcgatt tccccctcca tgcgcgttcg
     3541 ccggctcacc tgctccgagt ttgcctggtc cttcgacgac atcagggcgg aatcctcttc
     3601 cgcgcgggcc ttcaggccgg cttgcatcac ggcgagggat ttcagaagac gaccaagctc
     3661 atcgcgccgc cgaagctcga tccggtcggt aaagttgccg gcggctacat gttccgcaat
     3721 ctgcatggcg gcgaagatcg gtttgctcat ggagtgcgcg aaagcgaccg cgagcgccaa
     3781 cccgacgaga gccgttgcaa ccgcaagcgc gagcatggcg gcgtggtctg ccgcgaccgt
     3841 cgcctcggct tcagtgcgat agtcgaagcc ataagcagcg accatttcca caagatcgtc
     3901 cactgccgca gcggcggcat cgcctttctt ggccaccgag aaaggcgtgg gaagcgcggt
     3961 gaggccgccg gcctgcggct tgaggatctt cagccccgtg tccgaccagt cacgaagcct
     4021 cgtttcggcc tgctccagag cggcgatgac attcttggtt tttacccgat cccgaacaac
     4081 tttcaggtcg tccgtgatcc cggcgaccag tttctcgatt ttctcgaccg tctccttcgg
     4141 cgctcccgca ctgaggccag cctgcatcag aaggcgcgcg ccgttcaacg cggcatgcgc
     4201 cgaacgcgca tgattgattc ccatcagcgg tccatcgaag agccgcacta caagatcgcc
     4261 ggaggtggag atgccacgca tgccgtaaaa cgcaagcccg caggcaaggg cgatgacgac
     4321 actaaaggcg cagaaaaact tgtaacgaat tgacatagaa attcctccgg cgaacttcca
     4381 atggattagt ccgtaatctt caccgtcatc ttcattcgcg gatgaatggc gcagatgacg
     4441 agtgcttcgc cggattcctt gaaagtcaca tccgtcgccg cgcccggcgg ctgtgatccg
     4501 aggttgaact cgttcccctt actcgtcgaa atgatgttgt ggggcacgct gtcgtcgttt
     4561 gcgaaagtca gcgtctcgcc cttcttgatg gtgacggttt cggacgagaa gacgcggccc
     4621 ttctgggtta tcgtcaggtt ggcggccagc gcgccaaccg acaggccagc gagaacgaca
     4681 aaagtgagag aggctagctt gcgcattgga tgttcctgcg gttggcgttg actggttcag
     4741 cgggggagga ccggcacggt tgtcggatcg agattgctgg tcaaggtttt caggaaggcg
     4801 accaattcgg acttctcctg cggcgtgaga ttgagcggtt tcacgaggtc ggagcggctc
     4861 ggccggtcga cgcctccttg gtcataatgt tcgatcactt gttcgagcgt ggcgatcgac
     4921 ccatcgtgca tgtaaggacc tcgcctggcg atttcgcgca gccccggcgt cttgaaggcg
     4981 tgcttcatct tgatgacctg gggcaggaac tgcccccggc cgacatccgc gctcggaagg
     5041 ccgatgtcct ggaagccatc attggtgaag ttccagccct catggcacga tgagcactga
     5101 gccttggtgt tgaacaggac aaagccgcgc tttgcctcgt cggaaatcgc ttgctcgttt
     5161 ccctctatcc agaggtcgaa cggcgcctcc tcggaaacca cggttcgttc gtaggtcgca
     5221 atcgccgctg cgagcgactt gggcgataga ccggcgcctg gaaaggccgc cgcgaacagc
     5281 ggctggtatt cggcgatggt agccagccgc tccatcagtt gctcgatcgg catattcatc
     5341 tcgcctggag attggatcgg accgagcgcc tgctcctcaa ggttcgcaag ccgaccgtcc
     5401 cacatgaaga ttcctcccca tgcggcattg acgatggtcg gcgagtgccg gccgagcttc
     5461 gccatgccgt gaccgacccc gaccgccaat ccgtcgcccc aaccaaagcc cgggctatga
     5521 cagctcgcgc aggactgcgc tgaggtgacc gagatacggg tatcgaaata gagtttcttg
     5581 ccgagcgacg ctttgtcggg cgtgtaaggg ttttcttttg ggaacgggat caacgccgga
     5641 cgccggtact ctgccttcat cgcgtcaagg gctacagcgg ctttcgggct gggttcggtg
     5701 cgacgcatct ctccgacaat cgcagcacca aagccaaggt ttgtgaatcc gatcaagccg
     5761 agggcgaccg ccgctacgac cggaccggac cggacggaac gaatgaattc ggatggaagc
     5821 atgtttggtc cttgaagcgg cgttgggaga tgccacgtcc aagaaataac catcttttga
     5881 taatggcttc ataattttag cactcgtaca actactgggg agccgaagcc ggcgcttgtc
     5941 cggaccagcg ccccgctgtc atccgaattg tcgatcagct ttcaggtacc gtcgggcagt
     6001 accgcgttgc ttggcgtcgg acgatcggtg atctgcaagc cgaacaggct gccgagcagt
     6061 tccaccgcga cacgcgcggt gcggccacgt tcgtcgagaa agggattgag ctcgacgatg
     6121 tcgacagagc gcaccaatcc tgaatcgtgc aagagctcca tgacgagatg cgcctcgcga
     6181 taggtcgcgc cgcccggcac ggtggtgccg acgcccggcg ctactgccgg gtcgagaaaa
     6241 tcgacgtcga agctgacatg cagaacgccg ttgcgcgctt tgaccctttc gatgaccctc
     6301 cggatcagga cgccgacacc gaactcatcg atcgcgcgca tgtccgccac tgcgatgcgg
     6361 cgatcgcgga cgagatcctt ttcaagcgga tcgatcgagc ggattccgaa cagatcgagt
     6421 tgatcgggac tgatcgagac ccgcgccccg tcacccagca ggccatcgag gccgggctcg
     6481 ccgcagagga acgccgccga catgccatgc atgttgccgg tcacggtcgt cgccggcgta
     6541 ttgtagtcgg cgtgggcgtc gagccacagc acgaaaaccg gacggcccag ttcctgccag
     6601 tatcgcgcaa cgccgttgac cgatcccatc gacaggctgt gatcgccccc catgaagatc
     6661 ggaatggcgc ccgagcgcgc gagcagaaag gcgcgttcgc tgagcgcgcg tatccagcct
     6721 ttgatgtcat cataatattt ggcattcacc ggcggcggac catcggcgga cgaaaccgcg
     6781 tgcaccggga gattgccgtg atcctcgacg tcaaatccca attgctcaag cacgcggccg
     6841 atgcccgcgg tcctcaaggc atcaggcccc atcagcgtgc cgcgttgcga agccccgatc
     6901 tcgatgggaa caccgagcaa ggcgatctgc ggcgggccgg ggcttttcga tgtgccggac
     6961 atgatcggtg tgaccttcaa agctggaacg gaagaaacgc ctgcatgcag ttaagccttg
     7021 aagtacgcgg tttgatgcgt ggtggcgagc aggcgcccct gcggtgacca gagttccccc
     7081 gcctgatccc cataactttt gtggaagatt ttggcgtcgg cgacggcgag aacgcggtcg
     7141 atatcttccg ccgcaaggtc ctcggcgtcg acgtggaagt aagttgtcag cgataccgtg
     7201 ccaaacggca ccagttcgcg tctggcatga aacacgcggc cgaagaacgc atccgacatc
     7261 gacatcagcg acagcatgtc gatctttcgc ggaacccggt cgccgatcca cagtttggag
     7321 aacgcgtttg ccggcgcatc cttgaccgcg ccggagaaat cgggatggcc ctcgacgaag
     7381 cgaaaatcat attgcttgac ccacggcgct gccacctttg gatagggctg gatctgcgcg
     7441 aacggcggac ttttgggaaa ttcggccctt tggtgcgacc atgatgggcg gcgctcggca
     7501 aagacggcgg tcgcaagcgt cgcaacctcg gcgccgcctt ggctgagctc gacacgccaa
     7561 tgctgtgtcg accggttggc cttgatcagg cggacgtcga gatcgaaggc gccttgcgcg
     7621 atcggcgcgc aacagttgac ggtaaccgaa agcggatcgc ctgatcgttg cggatgatcg
     7681 atcagcgccc gcaggatggt ggcggcagtg attccgccga acgggccgac aaaagcccaa
     7741 taatcatcgc tggtccgccc ctgccagcgg ctgtcgccgg cggtcacccg ggtcgcgtcg
     7801 tcgaacaggt gctgggtctt ggtcagcatt tccgcacttt ccgtcatcgc gagccaatgg
     7861 gtcggtgcaa agcgccgccc gatgacaggc accgcgaagc aatccgtaag caacgaagca
     7921 agtctggatt gctcgtcgcg gagtttatca tcgggccggc cgaagaccgg acccgttggc
     7981 tgtgtgccga gcatgatcga cagcttggag gtgaaagtcc tttatccagc ctgatggtgg
     8041 cgaaggatta gcaaagcgca agggcgtcgt cgcgaggcgg ggtctgaagg aagcgtggag
     8101 caaag
//
LOCUS       DASWGM010000813         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2321748,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000813 DASWGM010000000
VERSION     DASWGM010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321748"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VGA15_22485"
     CDS             <1..262
                     /locus_tag="VGA15_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9590498.1"
                     /translation="SILLNNFSMAIELKVMPISTEKYRSTDISGDYAAMARAFGGHGE
                     RVTRPEDIIPAIRRGIAKTREGIPVLLEFITSKETEVSRPGT"
     gene            complement(291..671)
                     /locus_tag="VGA15_22490"
     CDS             complement(291..671)
                     /locus_tag="VGA15_22490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590499.1"
                     /translation="MRAFFLPIVLSALATPAGAQSLQVTGYSGYLGEWELTATVTENV
                     SGRTNEYSGPLTMKHVGLCTQDGPEEKTGEMRLQISALPSRLNATLLVAGVECTYSGR
                     LSDSYTGTMNCPDRGAVPLRLWVK"
     gene            complement(704..>1987)
                     /locus_tag="VGA15_22495"
     CDS             complement(704..>1987)
                     /locus_tag="VGA15_22495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590500.1"
                     /translation="AEGAAVLAKAMTVGAKTQRERDYLDALGAMYADYDKVDHRTRMQ
                     AYAKAMEQLAQRYPDDDEAQIYYALALNTSASPADKTYASQLKGAAILEPIAIRQPQH
                     PGVAHYLIHLYDYPPIAEKGLDAARRYAKVAPAAAHAQHMPSHIFTRVGYWKESIASN
                     VESSRVAKADKEGGDQLHAMDYLVYAYLQLGQDKKAAAVIDEMNAVTGFAETFIGGPY
                     ALAASPARYAVERGDWKAAAALQVRPSSLPHVQAVTHFARALGAARSGNPEAAKADIA
                     KLAELRDKLRDAKDAYWSEQVDIQRQVATAWLLYAEGKRDEALTSMSAAADAEDKTEK
                     HPVTPGVPRPARELYGVMLLESGNAKDALAAFEATIKKEPNRLGAYVGAAKAAEKSGE
                     SAKAQEYYSKVVTIADAADNSRTEVADARAFLTKR"
BASE COUNT          340 a          614 c          627 g          406 t
ORIGIN      
        1 gtcgatcctg ttgaacaatt tttccatggc gatcgaactg aaggtgatgc cgatttcgac
       61 cgaaaaatat cgctcgaccg acatctccgg cgactatgcc gccatggcgc gcgccttcgg
      121 cggccacggc gagcgggtca cgaggcccga ggacatcatc ccggcgatcc ggcgcggcat
      181 tgcgaagacc agggaaggca tcccggtgct gctggaattc attaccagca aggagacgga
      241 ggtttcgcgg ccggggacct gagcagctgc ggccattgac gccagcaata ctatttcacc
      301 catagcctga gcggaaccgc cccccgatcg gggcagttca tcgtgccggt ataggagtct
      361 gacaagcgcc cgctgtaagt gcattcgacc ccggcgacca aaagcgttgc attcaaccgg
      421 gacggtaagg ccgatatctg aagacgcatt tcaccggtct tctcctccgg gccatcctgg
      481 gtgcacaaac cgacgtgctt catcgtcaat ggcccggagt attcgttggt tcggccggat
      541 acgttttcgg tgacggtggc ggtcaactcc cactcgccga gatagcccga gtatccggtg
      601 acctgaagcg attgcgcgcc ggccggcgtc gccagagcac tcagaacgat cggtagaaag
      661 aatgctctca cgacgtgctc ccgtgctttc tttcggatgc ccctcagcgt tttgtcagga
      721 acgcgcgcgc gtcggcaacc tcggtccggc tgttatccgc agcatcggcg atcgtgacga
      781 ccttgctgta atactcctga gccttggcgg actcaccgga cttttcggcg gccttcgccg
      841 cgcccacata ggcgccgagg cggtttggct ccttctttat cgtcgcttca aaggcggcaa
      901 gcgcgtcctt tgcattgccg ctctcgagca gcatgacacc gtagagttcg cgcgccggcc
      961 tgggcacgcc cggcgtcacc ggatgcttct cggtcttgtc ctcggcatcg gcggcggcac
     1021 tcatggaagt cagtgcctcg tcgcgtttgc cctcggcata gagcagccag gcggtcgcga
     1081 cctgccgctg aatatctact tgctccgacc aataggcgtc cttggcatcg cgcaacttgt
     1141 cgcgcagctc ggcgagcttg gcgatgtcgg ccttggcggc ttcaggattg cccgagcggg
     1201 ctgcgcctag cgctcgcgca aaatgagtga ccgcctgcac gtgaggcagc gagctgggac
     1261 ggacttgaag cgccgcggcg gccttccaat cgccgcgctc cactgcgtag cgtgccggcg
     1321 aagcggccag cgcgtatggc ccgccgatga aagtctcggc gaacccggtg accgcgttca
     1381 tctcgtcgat aacggctgcg gctttcttgt cctgcccgag ttgaagatag gcgtagacca
     1441 ggtaatccat ggcgtgcagc tgatcaccgc cctccttgtc cgccttcgcg acacgcgacg
     1501 attcgacgtt ggaagcaatc gactccttcc aatagccgac gcgcgtaaaa atgtgtgacg
     1561 gcatgtgctg ggcatgtgcc gcggctggcg caaccttggc atagcgtctg gcggcatcga
     1621 ggcctttctc ggcgatcggc ggatagtcgt aaaggtgaat caagtaatgc gccacgccgg
     1681 gatgctgcgg ctggcgtatt gcgatgggct ccaggatggc cgcgcccttc agctggctgg
     1741 cgtaggtttt gtcggccggc gaagccgagg tgtttagggc gagcgcataa tagatctgcg
     1801 cttcgtcatc gtcgggatag cgctgtgcta gttgttccat ggccttggcg taagcttgca
     1861 tgcgcgtacg gtgatcgacc ttgtcgtagt cggcgtacat ggcgccgagc gcatcgagat
     1921 agtcgcgctc gcgctgggtc ttggcgccga cggtcattgc cttcgccagc acggcggcgc
     1981 cctcggc
//
LOCUS       DASWGM010000814         2561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_104012,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000814 DASWGM010000000
VERSION     DASWGM010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104012"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VGA15_22500"
     CDS             <1..765
                     /locus_tag="VGA15_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590501.1"
                     /translation="FRGGGFTPRAGRYSGWIALALAIGLWQLAGSAGWVNPLFLPAPS
                     AIAVAITRLAVSGALWHHLSVSVMRIGAGWILGTVAGVIVGFSIGLSSLARGVGITFI
                     SALFPIPKIALLPLLILWLGIGEEPKIATIALGVFFSTAISVYSGVDNVPRNLIRMAQ
                     SFNVPFHSIVRRVIWPGALPSILAGFRITASVALLLVVSAEMIGAEYGIGAFVLQAGN
                     LMQTDQLLAGVVILSLFGLAVGRLINLLETRLLHWR"
     gene            986..2059
                     /locus_tag="VGA15_22505"
                     /pseudo
     CDS             986..2059
                     /locus_tag="VGA15_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2190..>2561)
                     /locus_tag="VGA15_22510"
     CDS             complement(2190..>2561)
                     /locus_tag="VGA15_22510"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9590502.1"
                     /translation="IRALIEQWSKAVRDENRDAIRADHDAEILMFDVPPPFLSRGLYA
                     YMATWETFFASAEKPVSFAFHDVEVTCGADVAFATAVGRCVNIDTNGKREPLEFRLTM
                     GLRKIDGKWRIMHEHHSLPAT"
BASE COUNT          464 a          763 c          762 g          572 t
ORIGIN      
        1 tttcgcggtg gcggatttac gcccagagcg ggccgctatt ccggctggat cgcgctggcg
       61 ctggcgatcg ggctttggca actcgccggc agcgccggtt gggtcaatcc gttgtttctg
      121 ccggcgccgt ccgcaatcgc ggtcgcgatc accaggctcg cggtcagcgg cgcgctttgg
      181 caccatctgt cggtttcggt catgcgcatc ggagcgggct ggattctcgg cacggtcgcc
      241 ggcgtcatcg tcggcttttc gatcgggctc tcgagcctcg cgcgcggcgt cggcatcacc
      301 ttcatctcgg cgctgtttcc gattccgaaa atcgcgctgc tgccgctgct catcctgtgg
      361 ctcggcatcg gcgaggagcc gaagatcgcg accatcgcgc tcggcgtgtt cttttccacc
      421 gcgatctcgg tctatagcgg cgtcgacaac gtgccgcgca acctgatccg gatggcgcag
      481 agttttaacg tgccgtttca ttcaatcgtg cgccgggtga tctggccggg cgcgctgccg
      541 tcgatcctgg ccggattccg catcaccgct tccgtcgcgc tcttgctggt ggtgagcgcg
      601 gagatgatcg gcgccgaata cggtatcggc gccttcgtgc tgcaggccgg caacctgatg
      661 cagaccgatc agctgctcgc cggcgtcgtc atcctgtcgc tgttcgggtt ggcggtcgga
      721 cggctgataa atctgctgga aacacggctg ctgcactggc ggtaagtcga ccgtcattgc
      781 gaggaacaaa gcgacgaatt ttcgtgactc tggattgctt cgctgcgctc gcaatgacga
      841 tgttaagcgg catcatttcc gttcgtcatg ccctgtcttg tgccacggct gtccggctca
      901 ttttttgctg ttcgaggtgc acggcattga ttctaatggt gtccagagtt ttgcgactca
      961 actggacatg aaaaggatca gcgccgtgcc gcatacgaat actgtgtttc gcgacatttt
     1021 gaaacttgtt ccctgggccg cgttcgacga gcttgtgaag aagcatggga gcgacgaact
     1081 ggtgcgcagt tttacgacca aacgccagtt gctggcgctg ttgtatggcc aattgtcggg
     1141 cgcctgttcg ctgcgcgaca tcgaagcttc gatgaagagc catcaggggc ggctttatca
     1201 tgcgggcggc ttcgctactg cgcgctcgac cttcgcggac gccaaccgca gccgcaatcc
     1261 ggaggtgttt tcgggtcttt tcacgcatat gctgggcatg acgacgcgcg gcctgcgccg
     1321 caagttgggc gatgcggtgc gtctgatcga ctcgaccagc ctgcatttgg cgggcctcgg
     1381 cgctaattgg gcgcgctttt ccgccgatgt ctgcggcgcc aaggcgcatg tggtctatga
     1441 tcccgacctg ggctgtccga tctatcacat gatcaccgag gcgaacgtca acgatatcac
     1501 cgcggccaag gcgatgccga tcgaggctgg cgctacctac gttttcgacc tcggctatta
     1561 tgattatggc tggtgggctg cgctggacga cgccggctgc cgtatcgtga cgcggttcaa
     1621 gaaaaacacg ccgctccatt cggtcaaatc catgcccgtc gagcctggaa cgggagtttt
     1681 gagcgacagc atcggctttt tgccggggcg tcaggccaaa agccgcaaaa atcccatgca
     1741 aggcgcggtc cgcgagatcg tcgtgaccat ggagactggc gggactttgc gcattctgtc
     1801 caacgatctg gacgcacccg ccaaggaaat tgccgacctc tacaagcggc gctggcaaat
     1861 cgaactgttc ttccgtgtga tgaagcaaac cctgaaaatc acccacttta tcggccgctc
     1921 cgaaaacgct gttcgcattc agatcgccat cgcgctgatc gctttcctgc tgctgcgaat
     1981 attgaacaag atgtcacaag agaaactaac cttgcttgag actactagac tcgtgcgcat
     2041 caatctgatg catcgaaaag acttcacccg cctgaagcaa ttacataagc cgcccccacc
     2101 cgactgtcgc cagatcacgc taaactgggg gttaacttga accggacagc cgtgggcttg
     2161 tcccggccat ccacgtcttt gtcgctagac taagtggcag gcagcgaatg atgttcgtgc
     2221 atgatgcgcc atttgccatc aatcttgcgc agacccatcg tcagccggaa ttcgagcggc
     2281 tcacgcttgc cgttggtgtc gatgttgacg caccgcccta ctgccgtagc gaaggcgaca
     2341 tccgcaccgc aagtgacctc cacatcgtgg aaagcaaacg aaacgggctt ttcagccgat
     2401 gcgaagaatg tttcccaggt cgccatgtac gcatacaggc cccgcgacag gaacggtggc
     2461 ggtacgtcga acatgagaat ttcggcgtcg tggtccgcgc gaatggcatc tctgttttcg
     2521 tcgcgaaccg ccttgctcca ttgctcgatc agcgcgcgga t
//
LOCUS       DASWGM010000815         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2321893,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000815 DASWGM010000000
VERSION     DASWGM010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321893"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGA15_22515"
     CDS             <1..217
                     /locus_tag="VGA15_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590503.1"
                     /translation="NPTTGFFFYVPKSKIIEVDMSAEDAATLIMSAGVVQPGSDPQKK
                     IAALAEVANAARVAHESSLQPVPAKVD"
     gene            266..670
                     /locus_tag="VGA15_22520"
     CDS             266..670
                     /locus_tag="VGA15_22520"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9590504.1"
                     /translation="MHEAREHVAMLSPDEAKQLIDLGGVLLIDVGEEWQLRERGTIPG
                     ARNITRGELEIRADTEQERRDPALQDRQQKMILTCGGGGKATLSASALLEMGFTHVSV
                     IRGGCRGWQRAGYSLEPYPAPISGSASSQSNR"
     gene            695..997
                     /locus_tag="VGA15_22525"
     CDS             695..997
                     /locus_tag="VGA15_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590505.1"
                     /translation="MSFPRNTIAVWEVAELHRYATFSSMSPGTKTLLEQVQSWPEEDQ
                     EELAAVAREIESRRSGVYRLSDEERIAVRAGMDAARSGDFASDEEMDEFYRLHHRV"
     gene            994..1284
                     /locus_tag="VGA15_22530"
     CDS             994..1284
                     /locus_tag="VGA15_22530"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9590506.1"
                     /translation="MRVRFTRPAQRDLAQIHAYISQDSPDIASRFVARLIERSRKLAD
                     NPFEGRETDEPNARVIIAARLRYFIFYTVTADEIHITHIRHTSRRRPRGWTG"
     gene            complement(1311..3419)
                     /gene="recG"
                     /locus_tag="VGA15_22535"
     CDS             complement(1311..3419)
                     /gene="recG"
                     /locus_tag="VGA15_22535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX9590507.1"
                     /translation="MRPALLNPLFTPVTSLSGVGPKQDKLFRYLLGRDDTPRLVDLLL
                     HLPASVIDRRARPKIREAVPGTVVTLEVTVDRHRPTPGRNPRAPHLVYASDDTGDVVL
                     TYFRSQPGYVEKLLPVGAKRFVSGTLQMFDGIPQIVHPDRVVDEAGFAKLSGIDPVYP
                     LTEGLALGSLRRAMAQALQKLPALPEWISPEVIRRCKFPKIAEALNRVHMPVEMTDIL
                     PDGPFWSRLAFDELLAGQLALALVRAQLRRPAGDRNAGDGHLRNKIIDALPYSLTSSQ
                     QQAAAAISDDLRQPVRMLRLLQGDVGSGKTVVALLAAAAVTEVGKQAALMAPTEILAR
                     QHIKTITPLAERAGLRVAILTGREKGKERRELLARLEAGEIDLVVGTHALIQDDVVFK
                     ALALAVVDEQHRFGVRERLALTAKGEAVDVLVLSATPIPRTLVLTYFGDMDISELREK
                     PAGRQPIDTRAVPNSRLSEVMDGIGRALHAGKLVYWICPLVEESEAEGIEHLTNATER
                     YESLQERFGDRVGLVHGQMKGIEKDRVMAQFAAGEIGLLVATTVVEVGVDVPAATIMV
                     IENAERFGLAQLHQLRGRIGRGSEASTCLLLYKEPLNEMSTARLKVIRETTDGFRIAE
                     EDLKLRGEGDVLGVRQSGLPGYRIARTDVHAQLITQARDEALRIMKDNPRLKGERGEA
                     LRALLYLFERDEALPLIGAG"
     gene            3601..3957
                     /locus_tag="VGA15_22540"
     CDS             3601..3957
                     /locus_tag="VGA15_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HEX9590508.1"
                     /translation="MTEPRDSSLDVLLDLDGQVFVVDPEGGHWVRFVVTQVPASVEKP
                     HGLDYSLTLHGPHGERLVGFDNAHAVERQKRGQAQDHRHRLRTIKAYDYRDAATLLED
                     FWTMVDTVLRERGVTP"
     gene            3954..>4268
                     /locus_tag="VGA15_22545"
     CDS             3954..>4268
                     /locus_tag="VGA15_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9590509.1"
                     /translation="MTTLKVGIATYGEMKARTMAVARGERRMAAGEPKVWFTSTESFA
                     KVLSAGNRDLLRVIAEKAPASLDELSRITGKAKSNLSRTLKTMAGYGLVRLERGERGR
                     ITP"
BASE COUNT          824 a         1313 c         1355 g          776 t
ORIGIN      
        1 gaacccgacc acgggttttt tcttctatgt gcccaagagc aagatcatcg aagtcgatat
       61 gagcgccgag gacgccgcga ccttgatcat gtccgccggc gtcgtgcagc ccggctccga
      121 tccgcagaag aaaattgccg cgctggcgga ggtcgcgaat gccgcgcgcg tcgcgcatga
      181 atcctcgctg caaccggtgc cggcgaaggt ggattagggc tgagggacgc ctcgcatgcg
      241 caagggaaag acctttcgcg aacgcgtgca cgaagcgcgc gaacacgttg cgatgctatc
      301 tcctgacgaa gccaagcagc tcatcgacct cggcggcgtg ctgctgatcg acgtcggcga
      361 agaatggcaa ctgcgcgagc gggggacgat accgggcgcc cgcaacatca cgcggggcga
      421 gcttgaaatc agagcggata ccgaacaaga acgtcgcgat cccgcacttc aggatcgtca
      481 gcagaaaatg atattgacat gcgggggcgg aggaaaagcg accctctcgg ctagcgcctt
      541 gctggagatg ggttttaccc acgtgtccgt cattcgaggg ggctgccgcg gatggcaacg
      601 ggccggctat tcgctcgagc cttaccccgc cccgatcagc ggcagcgcct catctcagtc
      661 gaacagatag agcagggcgc gcagcgaagg tggattgagt tttccgcgaa acaccattgc
      721 cgtttgggaa gtggcggaat tgcacagata tgctacattt agcagtatga gccctggaac
      781 aaaaacgttg ttagagcagg ttcagtcctg gccggaagag gaccaggaag agcttgccgc
      841 cgtcgcacgc gaaatcgaat cgcgtcgcag cggcgtgtat cggctgtccg atgaggagcg
      901 aatcgcggtc cgtgctggaa tggatgccgc gcgcagtggt gattttgcgt ctgacgagga
      961 aatggacgag ttttatcgct tgcaccatcg tgtatgagag ttcgctttac ccggccggcg
     1021 cagcgcgacc ttgctcaaat tcacgcttat atcagccagg acagcccgga tatcgcctcg
     1081 cgttttgtcg ctcgtcttat cgaacgttcg cggaaactgg ccgacaatcc atttgaggga
     1141 agagaaacgg acgaaccgaa cgcacgcgta attatcgctg cccgtctgcg ttatttcatt
     1201 ttctacacgg tcactgcgga tgagattcac attactcaca tccgccatac atcgcgccgt
     1261 cgtccacgcg gctggaccgg ataacttttg ttcagctaat tacggatgca ctaccccgcc
     1321 ccgatcagcg gcagcgcctc atcgcgctcg aacagataga gcagggcgcg cagcgcctcg
     1381 ccgcgctcgc ccttcagcct gggattgtcc ttcatgatgc gcaatgcttc gtcgcgcgct
     1441 tgcgtgatga gctgggcgtg cacgtcggtg cgggcgatgc ggtagccggg caggccgctt
     1501 tgccggacgc caagcacgtc gccttcgccg cgcagtttca aatcctcctc ggcaatgcga
     1561 aaaccgtcgg tggtttcccg gatcaccttc agccgcgcgg tggacatctc gttcagcggc
     1621 tccttgtaca gcaacagaca cgtcgatgcc tcggagccgc ggccgatccg gccgcgcaac
     1681 tggtgcagtt gcgccagccc aaagcgttcg gcgttctcga tcaccatgat ggtggcggcg
     1741 gggacatcga cgccgacctc gaccacggtg gtcgcgacca atagaccgat ttcgccggcg
     1801 gcgaattgcg ccatcacgcg gtccttctca atgcctttca tctggccatg cacgagaccg
     1861 acacggtccc caaagcgctc ttgcaggctc tcgtatcgtt cggtcgcgtt ggtcagatgt
     1921 tcaatgccct ccgcttcgga ttcctccacc agcggacaaa tccagtagac cagttttccg
     1981 gcatgaagcg cgcggccgat gccatccatc acctcgctga gacggctgtt cggcaccgcg
     2041 cgggtgtcga tcggctgccg cccggcaggc ttctcgcgca gttccgaaat atccatgtca
     2101 ccaaaataag tcagcaccag cgtgcgcggg atcggcgtcg cgctcagcac cagcacatcg
     2161 accgcctcgc ccttggcggt gagcgccagc cgttcgcgca ccccgaaacg gtgttgttcg
     2221 tcgacgacgg cgagcgcgag tgccttgaac acgacgtcgt cctggatcag cgcgtgggtg
     2281 ccgaccacaa gatcgatctc gccggcttcg agccgcgcca gaagttcgcg gcgttccttg
     2341 cccttttccc gtccggtgag gatcgcaacc cgaagtccgg cgcgctcggc cagcggcgtg
     2401 atggtcttga tgtgctggcg cgccaagatt tccgtcggcg ccatcaacgc ggcctgcttg
     2461 ccgacctcgg tgacggccgc ggcggccaat agcgccacca cggtcttgcc cgatccgaca
     2521 tcgccctgca gcagccgcag catgcggacc ggctggcgca gatcgtccga tatcgcggcg
     2581 gcggcctgtt gctgggagga tgtcagcgaa tagggcagcg catcgatgat cttgttgcgc
     2641 agatggccgt caccggcgtt gcggtcgccg gcggggcggc ggagctgcgc gcgcaccagc
     2701 gccagcgcca attgaccggc cagcagttcg tcgaaggcga gccgcgacca gaacggacca
     2761 tcgggcagga tgtccgtcat ctcgaccggc atatgcaccc ggttcagcgc ttcggcgatt
     2821 ttgggaaatt tacagcgccg gatcacctcc gggctgatcc attccggcag tgccggcagc
     2881 ttttgcagcg cctgcgccat cgcgcgccgc agcgagccca gcgcgaggcc ttcggtcagc
     2941 ggatagaccg gatcgatgcc tgacagtttt gcaaaacccg cttcgtccac gacgcgatcg
     3001 ggatgaacga tttgcggtat gccgtcgaac atctgcaagg tgccggaaac aaaacgtttg
     3061 gcgccgaccg gcagcagctt ttcgacatag ccgggctggg agcgaaaata ggtcagcacc
     3121 acgtcgccgg tgtcgtcgct ggcatagacc agatgcggcg cgcgcgggtt gcgccccggc
     3181 gtcggacgat gccggtcgac cgtcacttcc agtgtcacca cggttcccgg caccgcctcg
     3241 cggatctttg ggcgcgcgcg gcgatcgatc acgctggccg gcagatgcag caacagatcg
     3301 accagccgcg gcgtgtcatc gcggccgagc aggtagcgaa acagcttgtc ctgcttcggc
     3361 cccacaccag aaaggctggt aaccggcgta aacaagggat tgagcagagc agggcgcatc
     3421 gggaaaactt aagaatcgga ctcgaaagcc gtcaagcaga ataggccgtt ttctcgtcat
     3481 tgcgaggcgc gacttgtccg ccgtagctca acgagcgaag gcggaagcga ccaaagcaat
     3541 ccatatacca ttccagccat tttccttgat ttggttgctt cattctgata accttctgag
     3601 gtgacggagc cgcgcgactc atccctggat gtgctgctcg acctcgacgg gcaggtgttc
     3661 gtcgtcgatc cggaaggcgg ccactgggtc cgctttgtgg tgacgcaggt gccggcgtcg
     3721 gtggagaagc cgcacggcct ggactattcg ctgacgctgc atggaccgca tggcgagcgg
     3781 ctggtcggct tcgacaacgc ccatgcggtg gagaggcaga agcgcgggca agcacaagac
     3841 catcgccacc ggctgcgcac gatcaaagct tacgactacc gggacgctgc gacgctgctc
     3901 gaagacttct ggaccatggt ggacactgtg ttgcgcgaaa ggggagtaac gccatgacaa
     3961 ctctgaaagt gggcatcgcg acctacgggg agatgaaggc ccgcacgatg gcggtggcgc
     4021 gcggcgagcg gcgcatggcg gccggtgaac caaaggtgtg gtttacctcg accgagtcct
     4081 tcgccaaagt tctgtcggcg ggcaaccgcg acctgctcag ggtgatcgcg gaaaaggcgc
     4141 ctgcctcgct cgatgaacta tcgcggatca ccggcaaggc gaagtcgaac ctctcgcgga
     4201 cgttgaagac gatggcgggc tacgggttgg tgcggcttga gcgaggcgag cggggacgga
     4261 tcacgccc
//
LOCUS       DASWGM010000816         5651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_368074,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000816 DASWGM010000000
VERSION     DASWGM010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368074"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..373
                     /locus_tag="VGA15_22550"
     CDS             206..373
                     /locus_tag="VGA15_22550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590510.1"
                     /translation="MTAEFVSFIRVGSKAGTELATADVQELGNAGHSAFTIASSPSVS
                     AAGPGCRISHG"
     gene            647..1045
                     /locus_tag="VGA15_22555"
     CDS             647..1045
                     /locus_tag="VGA15_22555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9590511.1"
                     /translation="MTPLEKVNSMKMPFAELKGVTFTEAERDRVVARMLVRPDLCTLN
                     HTLHGGAVMAFADSVGAAATVINLPEDAKGTTTIESKTNFIGGAKEGMTVIATATPVH
                     RGRRTQVWQTRLETEEGKLVAIVTQTQMVL"
     gene            complement(1072..2121)
                     /locus_tag="VGA15_22560"
     CDS             complement(1072..2121)
                     /locus_tag="VGA15_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldoxime dehydratase family protein"
                     /protein_id="HEX9590512.1"
                     /translation="MESAIPEHLRSARTRHRRVPDDYAPPYPSFVARHRPSVERVVMA
                     YFGVQTRGAPSNAAEQAMAGIASCFYSNGGPTHWDRARYVDEAGFTNVVTVAYWDDRA
                     KFDGWFPLARDGWTGDQRTSDEFGSFIEVLYPSVDGYETLFSSLGRCEGIAVLADSMS
                     GEVQEHAYWGGMRDRIPLSQTSEMAPAGAITAIRDGARIRVIPHDNVCLIRSGQDWGD
                     TEASERKMYLEDVEPVLREGMDFLRDDGRSIGCYANRYMTVVAPDGTPSEKSYGMSWW
                     KSLAALERWAESHPTHVRIFGAAMKYLSTLGPAAKLRLYHEVTVARADEQFFEYVDCH
                     PRTGMLNAVQTVEPA"
     gene            complement(2193..3206)
                     /locus_tag="VGA15_22565"
     CDS             complement(2193..3206)
                     /locus_tag="VGA15_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX9590513.1"
                     /translation="MGLVHQKYRVAVVQAAPVFLDLDATVDKTIALIEQAAAQGAKLI
                     AFPETFIPGYPWQIWLGAPAWAIGRGFVQRYFDNSLSYDSPQAEKIRKAVKRAKLTAV
                     IGVSERNGGSLYMGQWLIGPDGETIAKRRKLRPTHAERTVFGEGDGSDLAVHDRADVG
                     RLGALCCWEHLQPLSKYAMYAQNEQVHVGAWPSFSLYDPFAHALGWEVNNAASKVYAV
                     EGSCFFLGPCAVVSQAMIDELCDSPEKHAFLHAGGGHAVIYGPDGSSLAEKLPPDQEG
                     ILYADIDLGMIGVAKNAADPAGHYSRPDVTRLLLNTSRANRVEHFTLPVDAEVMSEIR
                     LQA"
     gene            complement(3410..4363)
                     /locus_tag="VGA15_22570"
     CDS             complement(3410..4363)
                     /locus_tag="VGA15_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9590514.1"
                     /translation="MAVVLDTSSTIPDRRLAVWQDIVCDTFVGLDCRSDMDEAFWGSV
                     SQSAVGPATFTRVDSCAQRVFRTPSRIARASEDFVLVALGNSGVNGVYQDGREAVISA
                     GQFTIYDTTRPYELRFDDSFSQTILQMPRKLLQQRIGSFDALTATTFSSDRPLERLAY
                     EFLLGMSKTIDQVDLATATRLLDQALDLVAMAFADRMRGRSPDQSFHRSALLYRLKNY
                     ILTHLRDPELSMPRAAAAIGISPRYASDLMAAEQTSFRSYVQLQRLERCKRDLSDPAH
                     AARHVSDIAFAWGFNDLAHFSRIFKQRFGASPRDWREHPTQ"
     gene            4570..5193
                     /locus_tag="VGA15_22575"
     CDS             4570..5193
                     /locus_tag="VGA15_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9590515.1"
                     /translation="MRHEDITRSTRARGNVRTLRQQEELPQLRDHLLRLDPQSRHDRF
                     HGFMDDDFIERYAAKCAVDGTVIIAYVEDGVVRGAAELHPPDHSPDSQAEIAFSVETC
                     VRRQGVGSILFRKLIAEARSKGYRCLRITTGAQNQAMRALANKFGAHLTFRHGESTGS
                     IDLRQQPQPEPAKLAIAAPAEAARAIVAFNRAYWKLLLRMYGWGRTA"
     gene            complement(5272..5574)
                     /locus_tag="VGA15_22580"
     CDS             complement(5272..5574)
                     /locus_tag="VGA15_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590516.1"
                     /translation="MSEDWNTKYGTRRVRRDPPTLAEAIFAAIGITDDQEAQAEIAAS
                     LMGLPIEAVQAEVKKVGRTAARSSTRVIAGEQGAQRSVVVERRVIRKFGTAKRTGT"
BASE COUNT         1116 a         1693 c         1790 g         1052 t
ORIGIN      
        1 gttgcgacgg actccactgt ctgactctcc ggcccggcgt gaaacaccga ccgtgctcaa
       61 tccgtcgaac cgccgtatac ggacccgtat gtacggtggt gtgggagggg aggagccgcg
      121 aggctccccc ctatcccgat cgccgcggct tctcgattga acacgaccgt ctctggaata
      181 ctgggtcgcc cggtcaagcc gggcgatgac agcggagttt gtctcattca tcagggtcgg
      241 ctccaaggcc ggtaccgaac tcgcgacggc tgatgttcag gagcttggca atgcgggcca
      301 cagcgcgttc acgatcgcgt ctagcccgtc ggtcagcgcc gcgggaccgg gctgcaggat
      361 cagccatggc tgacgatatg gcgaatgatg gataggttgt caccgaggcc gcaaatgaaa
      421 ggtcgtcgcc ggacttgacc cggcaggtaa ttgtcccaag aactcttgca cgaagataaa
      481 tcgtcattcc ggggcgcgcg tagcgcgaac ctcagatgtg ctattgcaca tcggggaatc
      541 tcgagattcc gggtctggtc cttcggacca tcccggaatg acgcgaacaa tttgagcgcg
      601 ggtcaagccc gcgtatgaca gctggagaaa acgggagcat tgcgagatga cgccgcttga
      661 aaaagtcaat tcgatgaaaa tgccttttgc ggagttgaag ggcgtgacat tcaccgaggc
      721 cgaaagggat cgcgtggtgg cgcggatgct ggttcggccg gatctctgca cgctcaacca
      781 tacgcttcac ggcggcgcgg tgatggcgtt cgccgattca gtcggggctg ccgcgaccgt
      841 gatcaattta cccgaggatg ccaagggcac cactacgatc gagagcaaga ccaatttcat
      901 cggcggcgcc aaggagggga tgacggttat cgcgaccgcg accccggtgc atcgcggccg
      961 gcgcacccag gtctggcaaa cgcggctgga gaccgaggag ggcaagcttg tggccatcgt
     1021 cacgcagacg cagatggtgt tgtagcaaag cggacgacgc gcgcccgcgc cttaggcagg
     1081 ctccaccgtc tgcacggcgt tcaacattcc ggttcgcggg tgacagtcga catattcgaa
     1141 gaactgctcg tcggcacggg cgaccgtaac ctcgtgatag agccgcagct tcgcggccgg
     1201 ccccagggtc gacagatatt tcatggcagc gccgaaaatc ctgacatggg tcggatgcga
     1261 ttcagcccat cgctccagtg cggccaggct tttccaccag ctcatgccat aggacttttc
     1321 gctcggcgtg ccgtcgggtg ctacgacggt catatagcgg ttggcgtaac agccgatcga
     1381 gcggccgtca tcgcgcagga agtccatgcc ttccctcaga accggctcga catcctcgag
     1441 gtacattttg cgctcggatg cttcggtgtc gccccaatcc tgaccggacc ggatcaggca
     1501 cacattgtcg tgcgggatga cccgaatgcg cgcgccatcg cgaatggcgg tgatagcacc
     1561 ggcgggagcc atctcactgg tctgtgacag gggaatgcga tcgcgcatgc caccccaata
     1621 ggcatgttcc tgaacctctc cgctcatgct gtcggcgagg accgcgatgc cttcacaccg
     1681 ccccaacgaa gagaacaggg tctcgtagcc atccacggat ggataaagca cctcgatgaa
     1741 gctgccgaat tcgtcgctgg ttcgttgatc gccggtccag ccgtcgcgtg cgagcggaaa
     1801 ccagccgtcg aactttgcgc gatcgtccca ataggcgacc gtcacgacgt tggtgaagcc
     1861 ggcttcgtcg acatagcgtg cccgatccca atgcgttgga ccgccattcg agtagaaaca
     1921 ggacgcgatc cccgccatcg cctgctcggc ggcgttcgaa ggcgcaccgc gggtctggac
     1981 gccgaaatag gccatcacca cccgctccac gctcggtctg tggcgcgcca cgaatgacgg
     2041 gtagggcggc gcataatcgt cggggacgcg ccggtgacgc gtacgggcgc ttcgcagatg
     2101 ctcggggatc gcggattcca tggcggcctc ctcttgaagt tcgctatagc gttttcgagc
     2161 gaagaaaacg cgtcagaaca aataggctct cctcaggcct gaagcctgat ttcgctcatg
     2221 acctcggcat cgaccggaag cgtgaaatgc tcgacgcggt tggcgcggga ggtgttgagc
     2281 agcagccgcg tgacgtcggg cctggaatag tgcccggccg gatcggcggc gttcttcgcc
     2341 acgccgatca tgccgagatc gatatcggca tacaggatgc cttcctggtc gggtggaagt
     2401 ttctcggcaa gcgagctgcc gtccggccca tagatcacgg catggccgcc accggcgtgc
     2461 aggaacgcgt gtttctcggg ggagtcgcag agctcatcga tcatggcctg ggagaccacc
     2521 gcgcaggggc cgaggaagaa acatgagcct tcgacggcgt agaccttgct cgcggcgttg
     2581 ttgacctccc agccgagtgc atgcgcgaat gggtcgtaga gcgaaaagct cggccaggcg
     2641 ccgacgtgaa cctgctcgtt ctgggcgtac atcgcgtatt tcgacagcgg ttgcagatgc
     2701 tcccagcagc acagggcgcc gagccgtccg acgtcggcgc gatcgtggac ggcgaggtcg
     2761 ctgccgtcgc cctcgccgaa cacggtgcgt tcggcatggg tcggcctcag cttgcggcgc
     2821 ttcgcgatgg tctcgccgtc gggaccgatc agccattgcc ccatatagag gctgccgccg
     2881 ttgcgttcgg acacgccgat gaccgcggtc agcttggcgc gcttcacggc tttacgaatt
     2941 ttttcggcct gcgggctgtc gtatgacagc gagttatcga aatagcgctg cacgaagcca
     3001 cggccgatcg cccaggccgg agctccaagc cagatctgcc acggatatcc gggaatgaag
     3061 gtctcgggaa acgcgatcag cttggcgccc tgcgcggcgg cttgctcgat cagggcgatc
     3121 gtcttgtcca ccgtcgcatc aagatcgaga aaaaccggcg ccgcctgaac caccgcaacc
     3181 ctgtatttct gatgaacgag acccatcggc gtcttccttt cgtattggga gacagacaat
     3241 cattggcccg aaaacataca gccatgcccg ggcgcgccgc ttgtccgtgg cagaacgaaa
     3301 acttgtctta attggcgaat ggcagcttta ggcttgctgc tcggcggcgt cgcgacgggc
     3361 agggcggctt ggtgccgcga cggaaagaaa acccgatcgg taaatgattt tattgcgtcg
     3421 ggtgttcgcg ccaatcgcgc ggcgaggcgc cgaacctctg cttgaagatt cggctgaagt
     3481 gggcgagatc gttgaaaccc catgcgaatg cgatgtcgct gacatgccgg gcggcatggg
     3541 cgggatcgga caaatcgcgc ttgcatcgct ccagccgttg cagctggacg taactgcgga
     3601 acgaggtctg ttcggcggcc atcaaatcgc tggcatagcg cggcgagatt ccgatggcgg
     3661 ccgccgcgcg cggcatcgac agttcgggat cgcgcagatg cgtcaggatg tagttcttca
     3721 gacgataaag cagcgcggat ctgtgaaaag actggtccgg cgaacgccca cgcatcctgt
     3781 cggcgaaggc catcgcgacg agatccagcg cctggtccaa aaggcgggta gcggtggcga
     3841 gatcgacctg gtcgatcgtc ttgctcatgc cgagcaggaa ctcataggcc agtcgttcga
     3901 gcggacgatc gctggagaaa gtggtcgcgg tcaacgcgtc gaacgagccg atgcgctgtt
     3961 gcagaagttt tcgaggcatc tgaaggatgg tctgcgaaaa gctgtcgtcg aagcgcagct
     4021 cgtagggacg ggtggtgtcg taaatggtga actggcccgc ggatatgacg gcttctcggc
     4081 cgtcctgata cacgccgttc acgccactgt ttcccaacgc caccagcacg aaatcctcgc
     4141 tggcgcgcgc gatccgcgac ggcgtgcgga acacccgctg ggcgcaggaa tcgacgcgtg
     4201 taaacgtcgc aggcccgacc gctgattgcg aaaccgaacc ccaaaacgcc tcgtccatgt
     4261 ccgaccggca atcgagcccg acaaaggtat cgcagacgat gtcctgccag acagcaagtc
     4321 gtcgatccgg aatcgttgaa ctggtgtcga gcacgacagc cacgatggac ctccaagaac
     4381 gccaagaatc cgccgctgca aacgtcaagg aaatcttaac gataacgaaa cgcgtgtggg
     4441 cgctaatatt ttggaattgt tgccgttatt tgaggcggga aatggtcttg aatatcatgc
     4501 tgcaatgcac aatattggcg gtctagggat tcgacgcctc ctcgagggcc atccaagagg
     4561 agtactaccg tgcgacacga agacattacc cgttcgacgc gtgccagggg caacgttcgg
     4621 acgctgcgtc agcaggagga attgccgcaa ctgcgcgacc acctgttgcg gctcgatccg
     4681 cagagccgac acgatcgctt tcatggcttc atggatgacg acttcatcga gcgctatgcc
     4741 gcgaaatgcg ctgtcgacgg tactgtcatc atcgcctatg tcgaggacgg cgtggtacgc
     4801 ggcgcggcgg agctgcatcc gcccgatcat tccccggatt cgcaagcaga gatcgccttc
     4861 agcgtcgaga cgtgcgtgcg acgccagggc gtcggcagca tcctgtttcg aaagctgatc
     4921 gcggaagcgc gatcgaaggg ttaccgctgc ttgcggatca ccaccggtgc gcagaaccag
     4981 gcgatgcggg cgctcgccaa caagttcggc gcgcatctga cattccgcca cggcgagtcc
     5041 accggcagca tcgatctaag gcagcaaccg cagcccgaac cagcaaagct tgcgattgct
     5101 gcgccggccg aagccgcacg cgcgatcgtt gccttcaatc gcgcgtactg gaagctgttg
     5161 ctgcgaatgt atggctgggg ccggacggcc tgaccagcgg cgcctgcatg cagcgatcgc
     5221 cgatccctga tcaggcgatt ggccgagaca gcggcttgcg ctgaatgcgc gtcaggttcc
     5281 ggtgcgcttg gcggtgccga atttcctgat cacgcggcgc tcgaccacga cggaacgctg
     5341 cgcgccttgc tcgcccgcga tcacccgcgt cgaagaccgt gccgccgtgc ggccgacttt
     5401 cttcacttcg gcttgaacgg cttcgatcgg caaccccatc agcgacgctg cgatttcagc
     5461 ctgggcctcc tgatcgtcgg tgatgccgat cgctgcgaag atggcttcag ccagcgtcgg
     5521 cggatcgcgc cggacgcgcc gcgttccata tttggtattc caatcttcgc tcataggggc
     5581 cccttgtttg atccgaaata cctaggggtg atgatgttgc attgcaatat gaaattgatg
     5641 tcgcaatcca g
//
LOCUS       DASWGM010000817         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1891668,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000817 DASWGM010000000
VERSION     DASWGM010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891668"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VGA15_22585"
     CDS             complement(<1..573)
                     /locus_tag="VGA15_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX9590517.1"
                     /translation="MASTNLQSTSSDYRAEMDQAERASRDEIIALQTRRMAWTLAHAY
                     DNVAHYRKAFDKAGVTPQDFRQLSDLSQFPFTAKADLRQNYPFGLFAVPREKLVRIHA
                     SSGTTGKPVVVGYTKADIDMWSDVMARSIRAAGGRTGMILHNAYGYGLFTGGLGAHYG
                     AERLGCTVVPVSGGMTERQVQLINDFRPDII"
     gene            complement(612..1076)
                     /gene="paaI"
                     /locus_tag="VGA15_22590"
     CDS             complement(612..1076)
                     /gene="paaI"
                     /locus_tag="VGA15_22590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEX9590518.1"
                     /translation="MNVKAPLSPDDLARACADAMWQDDDASKDLGMEIRQIRPGEATL
                     TMTVKPQMVNGQGIAHGGFIFLLADSAFAFACNSHNERAVAAQCNISFIRPGKLGDLL
                     IATAREISRTGRSGIYDVRVTVDDTPIAELRGHSRTIGGAWVPTADPDARLK"
     gene            1317..2324
                     /locus_tag="VGA15_22595"
     CDS             1317..2324
                     /locus_tag="VGA15_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX9590519.1"
                     /translation="MDGPPVTVEARCVRLLAKADDPASLAPVIERRPLSRAANELLIE
                     VKAAAVNPSDVKAATGLMAYAVFPRTPGRDYAGIVIDGPADWIGREVFGSSGDLGIRR
                     DGTHATHLAVEADAVVEKPKSLSWDEAAGIGVPFVTAMEGFRRAGMPAKDDTVLVMGV
                     NGKVGQSAVQIASWNGARVIGVVRKREPYEGHTNSEVEVIDASAQDVATRVRELTDGK
                     GADIVFNTVGDPYFQAAHKSLAVRGRQILIAAINPIVQFNILEFYRGQHSYVGIDTLG
                     LSSTATGAVLKELVPGFASGHLKPFPIKAGAIYPLEEAKAAFVAVAGSSRDRVILRPS
                     P"
     gene            2340..>2465
                     /locus_tag="VGA15_22600"
     CDS             2340..>2465
                     /locus_tag="VGA15_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX9590520.1"
                     /translation="MPSGAHIVILAGLLIGLVYGGVGLLSGFCLMSSLRGWWAEGD"
BASE COUNT          434 a          752 c          815 g          464 t
ORIGIN      
        1 gatgatgtcg ggcctgaaat cgttgatcag ctgcacctgc cgctcggtca tgccgccgga
       61 aaccggcacc acggtgcagc cgagcctttc ggcgccgtaa tgcgcgccga ggccgccggt
      121 gaacaggcca tagccatagg cgttgtgcag gatcatgccg gtgcggccgc cggcggcgcg
      181 gatcgagcgc gccatcacat ccgaccacat gtcgatatcg gctttggtat atccgaccac
      241 gaccggcttg ccggtggtgc cggaggaggc gtgaatccgg accagttttt cgcgcggcac
      301 ggcgaacaag ccaaacggat agttttggcg cagatcggct ttcgcggtga acggaaattg
      361 gctcaaatcc gacagttggc gaaaatcctg cggggtaacg ccggccttgt cgaatgcttt
      421 ccggtagtgt gcgacgttgt cgtaggcgtg cgccagcgtc caggccattc gcctggtctg
      481 cagcgcgatg atttcgtcgc gcgatgcgcg ctcggcctgg tccatctcgg cgcggtaatc
      541 gctgctcgtg gattgaaggt tggtcgaagc catcgcgcgt ttctcctgaa atcagcgatt
      601 cagtgcagcc ttcatttcaa cctggcatcg gggtccgccg tcggcaccca ggcgccgccg
      661 atggtgcggg aatggccgcg cagctcggca atcggggtgt cgtcgacggt gacgcgcaca
      721 tcgtaaattc cggaacgccc ggtgcgcgag atttcccgcg cggtcgcgat caggaggtcg
      781 ccgagcttgc ccggccggat gaaggagata ttgcactgcg cagcaaccgc gcgttcgttg
      841 tgggagttac aggcaaaggc gaacgccgaa tccgccagca ggaagatgaa gccgccatgg
      901 gcgatgccct ggccgttgac catctgcggc ttgaccgtca tcgtcagcgt ggcttccccc
      961 ggcctgatct gccggatctc catcccgaga tccttgctgg cgtcgtcatc ctgccacatc
     1021 gcgtcggcgc aggcgcgtgc gagatcgtcg ggcgaaagtg gggccttgac gttcaccgtg
     1081 gtctctccgc gcgtttccgc ggccgcctga cgatcaggat cggatccaag acgttctccc
     1141 caagacgttc tccgagctcg ccgccgggct tgtaggaata agcctcttga actcagcgat
     1201 cagtccgtta gtcatgatgc gaagcgacca tggtgtcaac gaggccgtca ggcatcaaat
     1261 cgcgttcgaa agctgcaagg aaagcgtgaa catccgtgat tccagccccc caaaaaattg
     1321 atggcccgcc ggttaccgtc gaggcgcgct gcgtgcgtct gctcgcgaag gccgacgatc
     1381 cggcgtcgct tgcccccgtg atcgagcggc ggccgctgtc gcgggccgcg aacgagctgt
     1441 tgatcgaggt caaggcggcg gcggtcaatc cgtccgacgt caaggccgcc accggcctga
     1501 tggcttacgc ggtatttccg cgcacccccg gccgcgacta tgcggggatt gtgatcgacg
     1561 gcccggccga ttggatcggg cgcgaggtgt tcggctcgtc gggcgacctc ggaatccggc
     1621 gcgacggcac ccatgccacg catctcgcag tcgaagccga cgccgtggtg gagaaaccga
     1681 aaagcctgtc gtgggatgaa gccgccggta tcggcgttcc cttcgttacc gcgatggaag
     1741 gttttaggcg cgccggaatg cccgcgaaag acgacacggt gctggtgatg ggcgtcaatg
     1801 gcaaggtcgg tcaatccgca gtgcagatcg cgagctggaa cggcgcgcgc gtgatcggcg
     1861 tggtgcgcaa gcgcgagccc tatgagggcc acaccaattc agaagtcgaa gtgatcgacg
     1921 catcggcgca ggacgtcgcc acgcgggttc gcgaactcac cgacggcaag ggcgccgaca
     1981 tcgtgttcaa caccgtcggc gatccctatt ttcaggcggc gcacaaatcg ctggcagtgc
     2041 ggggacgtca gatcctgatt gccgcgatca atccgatcgt ccagttcaac atcctggaat
     2101 tctatcgcgg ccagcatagc tatgtcggca tcgacaccct ggggctgtcg tcaaccgcga
     2161 ccggcgcggt tctgaaagaa ctggttcccg gttttgcgag tggacatctc aagccgttcc
     2221 cgatcaaggc gggtgcgatc tatccgctgg aggaggcaaa agctgccttt gtcgcggtcg
     2281 ccggctcatc gcgcgatcgc gttattctgc ggccctcgcc ataagaaggg acaaagtcgt
     2341 tgccgtcagg tgcccacatc gtgatccttg ccggcctgct gatcggcctc gtctacggcg
     2401 gggtcgggct attgagcggg ttttgcctga tgagcagcct gcgcggctgg tgggcggagg
     2461 gcgac
//
LOCUS       DASWGM010000818         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2471513,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000818 DASWGM010000000
VERSION     DASWGM010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471513"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..474)
                     /locus_tag="VGA15_22605"
     CDS             complement(13..474)
                     /locus_tag="VGA15_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEX9590521.1"
                     /translation="MMMWRGPFAKTGPLKILMVGLALAWTASQMAPARAEATASAPGA
                     VLYFHYPSDGARVPQRFTVKIGLKEMGVAPAGVVKEFTGHHHLLVDSDMVPLDQPIPS
                     DYNHIHLGNGQTETVLTLPPGTHTLQLLLGDHQHIPHQPPVMSKKITIYVR"
     gene            complement(507..1757)
                     /locus_tag="VGA15_22610"
     CDS             complement(507..1757)
                     /locus_tag="VGA15_22610"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEX9590522.1"
                     /translation="MVRVFFRVCLIVLATITLDARIEVGAQQPAPARKPSAPNARIFF
                     VDLKDGATIPSKIKIRFGIENMEIAPAGTAKPNTGHHHLLIDTELPPLDQPIPSDFNH
                     IHFGGGQTEAEITLPAGEHTLQLLLGDQAHISHQPPVFSEVLHVRVDPATVEKARTPA
                     PVGAGVFFVEPADGATVGRKVTVKFGLSGMELVPAGTNKPNSGHHHLLIDAPLPLLDR
                     EIPSDLNHVHFGRAQTEAEINLTPGDHTLQLLLGDHEHLPHDPPIFSKVIRIHVADTA
                     SDKAPQAGPTADRRVAPPDAAVYFIYPSNGERIYPNSTIRFGLRGMGVAPAGVVKPNT
                     GHHHLMIDVETPPLDRPLPSDLNHIHFGSGQTEKKITLSPGEHTLQLILADEKHMPHD
                     PPVVSERIKVTVKARPVRKRFRRW"
     gene            1994..>2683
                     /locus_tag="VGA15_22615"
     CDS             1994..>2683
                     /locus_tag="VGA15_22615"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9590523.1"
                     /translation="MNILVRSLAFGFLFAVGLFAEASAAEQRVALVIGNGGYWSAPEL
                     RNSRNDADDISAQLKQLGFAVIDGRDLDRSSMQAALSRFAQRLKGADAGLVYYSGHGL
                     QMNGQNFLVPVDLKYDNGTFTPFELVKVDDVIDALGYTDGVRLLVLDACRDNPFANSL
                     AQNKGTRGAGTTRGLAKIERSQGMLIAYSTQPNAVAADGTGRNSPFTAALVRELQVPG
                     LEVATVFRRVAV"
BASE COUNT          521 a          818 c          806 g          538 t
ORIGIN      
        1 atcgccgttc ggttagcgca cataaatcgt gatcttcttc gacatgacgg gaggctgatg
       61 cgggatgtgc tggtggtcgc cgagcagcaa ctgcaatgtg tgcgttcccg gaggcagcgt
      121 cagcaccgtc tcggtttgtc catttcccaa atgaatatga ttgtaatcgg aggggatcgg
      181 ctgatccagc ggcaccatat cgctgtcgac cagcaggtga tggtgcccgg tgaattcctt
      241 gacgacgccg gcgggggcca cgcccatctc cttcaacccg atcttcaccg tgaagcgctg
      301 gggcacgcgc gcgccgtcgc ttggatagtg aaaatagagc accgcccccg gcgcggacgc
      361 ggtggcctcg gcccgcgccg gcgccatctg cgacgccgtc cacgccagcg ccaacccgac
      421 catgaggatc ttcaaaggcc cggtcttcgc aaatggtcca cgccacatca tcatttcacc
      481 tgttccccat ctgcgaaatc tcttcctcac cacctgcgaa atctcttcct caccggccgc
      541 gccttgacgg tcaccttgat tcgttcggag accaccggcg gatcatgcgg catgtgcttt
      601 tcgtcggcga ggatcagttg aagcgtatgc tctcccgggg aaagcgtgat tttcttctct
      661 gtctgtccgc tgccgaaatg aatgtgattc aagtcactcg gcagcggccg atccagcggc
      721 ggggtctcga catcgatcat caggtgatga tggccggtat tcggcttgac gacgccggcc
      781 ggcgccacgc ccatgccacg caggccgaac cggatcgttg agttcgggta aatgcgctcg
      841 ccgttgctcg gatagatgaa ataaaccgcg gcgtccggcg gcgcaacgcg acggtcggcc
      901 gttggcccgg cctgcggagc cttgtccgac gccgtatccg cgacatggat gcgaatgacc
      961 ttcgagaaga tcggcggatc gtggggaaga tgttcgtggt cccccagcaa cagctgcagc
     1021 gtgtgatccc cgggcgtcaa attgatttcc gcctcggtct gggcgcgacc gaaatgcaca
     1081 tggttcaggt cgctcgggat ctcgcgatca agcaagggaa gcggggcatc gatcagcaag
     1141 tgatgatgtc cgctattggg cttattggtc cccgccggca ccagctccat gccggagaga
     1201 ccgaatttca cggtcacctt gcgtcccacc gtcgcgccat ccgccggctc gacgaaaaac
     1261 acgccggcgc ccacaggcgc aggggtccgg gccttttcga cggtcgccgg atccaccctg
     1321 acgtggagga cctcggaaaa taccggcggc tggtgtgaaa tgtgagcctg atcacccaac
     1381 aggagctgca aagtatgctc acctgcgggc agggtgattt ccgcctcggt ctgaccgccg
     1441 ccaaaatgaa tgtggttgaa atcgctcggg atcggctgat cgagcggcgg tagttcggta
     1501 tcgatgagaa ggtgatgatg accggtattt ggcttcgcgg tgccggcagg agcgatctcc
     1561 atgttctcga tcccgaaacg gatcttgatc ttggagggga tcgtcgcacc gtccttgagg
     1621 tcgacaaaga atatcctggc attcggagcc gaaggcttcc tcgccggagc cggttgctgt
     1681 gcgccaacct cgatacgcgc atccagagtt attgtggcaa gaacaatcag gcagacgcgg
     1741 aaaaaaactc gaaccataag cagctcccct gtcacgctaa catgagcctc caaggcgaac
     1801 aagttgccgt agcagaccat cgctccgaag tcccaaagct gctgcgtgac gaggctcacg
     1861 ctttgctgca aatggcttgc tgcaacaagc cctgctgcaa aaagcgttgg cgaacagatc
     1921 gttcgcggct cgcgtggccg ggttgttcgt tgaaaacagg cctcctttcg gatagactgg
     1981 cggaattatg cgcatgaaca tattggtccg atctctcgct tttggtttcc tgttcgccgt
     2041 cggccttttc gccgaggcgt ccgccgccga acaacgtgtc gccctggtga tcggcaatgg
     2101 cggctactgg tcggcgccag aacttcgcaa cagccggaat gatgccgacg atatttccgc
     2161 gcaactcaaa caattgggtt ttgccgttat cgacggccgc gatctcgaca ggtcgtcgat
     2221 gcaggccgca ctcagccgct ttgcccagcg cctcaagggc gccgacgcgg gcctcgtcta
     2281 ctattcggga cacgggctgc agatgaacgg tcagaacttt ctcgttccgg tcgatttgaa
     2341 gtacgacaac ggcacgttca cgccgttcga actggtcaag gtcgacgacg tcatcgatgc
     2401 gctcggctat acggatggtg tccggctact ggtgctggac gcctgccgcg acaacccatt
     2461 cgcgaacagt ctcgctcaaa acaaaggcac ccgtggcgcc ggcacgacgc gaggccttgc
     2521 caaaatcgaa cgctcgcagg ggatgctgat cgcctattcg acgcaaccca acgccgtcgc
     2581 cgccgatggc accgggcgca acagcccttt caccgcggca ctggtgcgcg aacttcaggt
     2641 tcccgggctg gaggtcgcga cggtatttcg ccgtgtcgcg gta
//
LOCUS       DASWGM010000819         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2047192,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000819 DASWGM010000000
VERSION     DASWGM010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047192"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VGA15_22620"
     CDS             complement(<1..637)
                     /locus_tag="VGA15_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX9590524.1"
                     /translation="MTHHHLHSSPETCHWGFFEAKLKPVLTIASADEVTVDTISGGPD
                     VVPDRGKFHVPPELAEVHAKSERMLPGHILTGPIAVEGAEPGHVLEVEILDVRLRQDW
                     GYNMIRPLAGTLPDDFHESRLLNIPLDAKRMVGRLPWGLDLPLKPFFGVMGVAPPPAW
                     GRITSLIPRAMGGNLDNKELTPGAKLYLPVFVPGALFSCGDGHGVQGDGEVC"
     gene            874..1662
                     /locus_tag="VGA15_22625"
     CDS             874..1662
                     /locus_tag="VGA15_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEX9590525.1"
                     /translation="MPQAATQEEKIDLAGMVADLNSLLRLKTTVIGMKMFARAEDMAA
                     IPKIRRPTAVHTTDQIVSMASRLGWTVGITADDLVGAQCRAVIGLAPQDEKWLAGQAY
                     VGIWHENAEDARKRQEALDVVPHGRYEALVVSPLASGRLDPPDICLVYATPGQMIILI
                     NGLQWTGYKKFEWGVVGETACADSWGRALKTGEPSLSLPCYAERRYGGVPDEEMLMAL
                     SPAHLAKAITGMKALAKNGLRYPIAPYGIQSDVRAGMGVSYGKK"
     gene            1835..3016
                     /locus_tag="VGA15_22630"
     CDS             1835..3016
                     /locus_tag="VGA15_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEX9590526.1"
                     /translation="MSSSKFDIVVYGATGFTGQLVAEYLAAHYTGKAAPKWAMAGRSL
                     DKLASVRDAIGAPADIPLIAADAGDPASLRAMVDQANLVLTTVGPYQYYGSELVAACA
                     ATGTDYLDLCGEPVWMRQMIDAHEATARNTGARILFSCGYDSIPFELGVFFCQETAKK
                     LLGAPVSRIKGRVRVMKGTLSGGTAASMKAIFSAAANDLSLVALLRNPFALTPGFEGP
                     KQPPGNKPLYDGDLESWTTPFVMANINTRNVHRSNMLMGFPYGRDFVYDEMVLTGPGE
                     QGEESARRILAANTSEKMGSGGPKPGEGPSKEERESGHYDLLFVGLAPDGRQVRVAVR
                     GDRDPGYGSTSKMIGECAICLLRDTPEIPGGIWTPGAAMGVSLIKRLVDHAGLTFDVE
                     S"
     gene            complement(3132..>3304)
                     /locus_tag="VGA15_22635"
     CDS             complement(3132..>3304)
                     /locus_tag="VGA15_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase domain-containing protein"
                     /protein_id="HEX9590527.1"
                     /translation="AIFAAFLGTVGESFIIDTDHWRHFWMMLGAMWGLFAAAQRYRQT
                     SDQALTTTAQAS"
BASE COUNT          626 a         1013 c         1080 g          585 t
ORIGIN      
        1 cgcagacctc gccatcgccc tggacgccat gaccgtcgcc gcaggaaaac agcgcgcccg
       61 gcacgaacac cggcagatag agtttggcgc ccggcgtcag ttccttgttg tcgaggttgc
      121 cgcccatcgc gcgcgggatc agcgaggtga tgcggcccca ggccggcggc ggcgccacgc
      181 ccatcacgcc gaagaacggt tttagcggca gatcgaggcc ccatggcagc cgccccacca
      241 tccgcttcgc gtcgagcgga atattcaaaa gccgcgactc gtggaaatcg tccggcagcg
      301 tgcccgccag cggccggatc atgttgtacc cccaatcctg cctgagcctg acgtcgagga
      361 tctcaacctc cagcacgtgg ccgggttcgg cgccttccac cgcgatcggg ccggtcagga
      421 tatggcccgg cagcatccgc tcgctcttgg cgtgaacctc ggcgagttcg ggcggaacgt
      481 gaaatttgcc gcggtccggg accacgtccg gaccgccgct gatggtgtcg acggtgactt
      541 cgtcggcact ggcgatggtg aggacgggtt tcagcttggc ttcgaaaaag ccccaatggc
      601 aggtctcggg gctggaatgc agatgatgat gcgtcatgag ggggacttcg ggttgggggc
      661 aggggaggtg agtgtgccct cggcgaggcg ttctgtcgat gttcctactg ttattgccgg
      721 gcaaaaggcg ccaagcgccg tcttcgcgcc tgatgaccgt caatccaaca tcttcgcgta
      781 aagctctttg tgaaaatgga tggatacgcg ggtcaagccc gcgtatgacg ctgaatgagc
      841 agccatcagc ctgccgttca gggagtgtca gtcatgccgc aagcagccac gcaggaggaa
      901 aaaatcgatc tggccggcat ggtggcggat ctgaacagcc tgcttcgcct gaagaccacc
      961 gtgatcggca tgaagatgtt cgcgcgggcg gaggatatgg cggcgatccc gaagatccgg
     1021 cggccgacgg cggttcacac caccgaccag atcgtcagca tggcctcgcg gctgggctgg
     1081 accgtcggca tcaccgccga cgatctcgtc ggcgcgcaat gccgcgccgt gatcggcctt
     1141 gcgccgcagg acgagaaatg gctcgccggt caagcctatg tcggcatctg gcacgaaaat
     1201 gccgaggacg cgcgcaagcg ccaggaggcg ctcgatgtcg ttccccatgg ccggtacgaa
     1261 gcgttggtgg tgagcccgct cgccagcggc cggctcgatc cgcccgatat ctgcctggtc
     1321 tatgcgacgc cgggccagat gatcatcctg atcaacggcc tgcaatggac cggctacaag
     1381 aagttcgaat ggggcgtggt cggcgagacc gcgtgcgccg attcatgggg ccgggcgctc
     1441 aagaccggcg agcccagcct gtcgctgccg tgctatgccg agcggcgcta tggcggcgtg
     1501 ccggacgaag agatgctgat ggcgttatcg cctgcgcatc tggccaaggc gatcacgggc
     1561 atgaaggcgc ttgcgaagaa cggcctgcgc tatccgatcg cgccctatgg tatccaaagc
     1621 gatgtgcgcg ccggcatggg cgtttcctac ggcaagaaat aggttcaaat ggagtagacc
     1681 gtcattgcga ggagccaacg ggtccggcct tcggccggcc cgatgataaa ctccgcgacg
     1741 aagcaatcca tctttctgtg cgggaccgct atggattgct ttgcttcgct cgcaatgaca
     1801 gaagaaccca acagtcacct caggaaattc gcatatgtct tcgtcgaaat tcgacattgt
     1861 cgtctatggc gccaccggct ttaccgggca actcgtcgcc gaatatctcg ccgcgcatta
     1921 caccggcaag gccgctccga aatgggcgat ggccggccgc agcctggaca aactcgcttc
     1981 cgtccgcgat gcgatcggcg cgcctgccga tattcccctg attgccgccg acgccggcga
     2041 tccggcttcg ctgagggcga tggtcgacca ggccaatctg gtgctgacca cggttggtcc
     2101 ctatcagtac tacggttcgg aacttgtcgc ggcctgcgcg gccactggta ccgattacct
     2161 cgatctctgc ggcgaaccgg tctggatgcg ccagatgatc gatgcgcatg aggccacggc
     2221 aagaaacacc ggcgcccgca tcctgttttc ctgcggctac gattcgatcc cgttcgaact
     2281 cggggttttc ttttgccagg agaccgcgaa aaagctgctg ggcgcgccgg tcagccggat
     2341 aaagggacgg gtccgcgtca tgaagggcac gctgtcgggc ggcaccgcgg cgagcatgaa
     2401 agcgattttc tctgccgcgg ccaacgacct cagtctggtg gcgctgttgc gaaatccgtt
     2461 cgcgttgacg ccgggctttg agggcccaaa gcagccaccg ggcaacaagc cgctctacga
     2521 cggggatctg gaatcgtgga cgacgccttt tgtgatggcg aacattaaca cccgaaacgt
     2581 gcaccgctcc aacatgctga tgggctttcc ctacggccgg gattttgtct atgacgaaat
     2641 ggtgctgacc ggcccgggcg agcagggcga agagagcgcc agacggatcc tcgcagccaa
     2701 cacttcggag aagatgggca gcggcgggcc gaagcccggc gagggcccgt cgaaggaaga
     2761 gcgcgagtca ggccactacg atctcttgtt cgtcggcctt gcaccggacg gacgtcaggt
     2821 gcgcgtcgcg gtgcgcggcg atcgcgatcc cggctatgga tcgacctcga aaatgatcgg
     2881 ggaatgcgcg atctgcctgc tgcgcgacac gccggaaatt ccaggcggca tctggactcc
     2941 cggcgctgcc atgggcgttt ccctgatcaa gcggcttgtc gatcacgccg gtctgacgtt
     3001 tgacgtcgag agctagatcc cgcggtgcgg ggatcatagg cgtacatcca gtccatgcta
     3061 caaaacgatg ctggctgcga ccgaggaact gaaggcgatg atcgaagcgc cgaagcgcaa
     3121 gcgcagcggc gctaagaagc ctgcgcggtt gtcgtgagcg cctgatccga agtctgccgg
     3181 tagcgctgtg cggcggcgaa caggccccac atggccccca gcatcatcca gaagtgccgc
     3241 cagtggtcgg tgtcgatgat gaagctttcg ccgaccgtgc ccaggaacgc cgcgaagatc
     3301 gcaa
//
LOCUS       DASWGM010000820         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2472192,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000820 DASWGM010000000
VERSION     DASWGM010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472192"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VGA15_22640"
     CDS             complement(<1..110)
                     /locus_tag="VGA15_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HEX9590528.1"
                     /translation="MSKTLDEQDLGRPLKRAFFARSVHEVAPDLIGATLLV"
     gene            328..1287
                     /gene="lipA"
                     /locus_tag="VGA15_22645"
     CDS             328..1287
                     /gene="lipA"
                     /locus_tag="VGA15_22645"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX9590529.1"
                     /translation="MVVVVDTVSATPLRPRHPEKVNRPDALSPPKPDWIRVRAPNTRG
                     YADTRQIVRENGLVTVCEEAGCPNIGECWDKKHATFMIMGDTCTRACAFCNVKTGMPG
                     ALDAHEPEHVAEATFKLGLAHIVITSVDRDDLADGGAEHFARTIGAIRARCPTTTIEI
                     LTPDFLRKQGALETVVAAKPDVFNHNLETVPSRYLTVRPGARYFHSIRLLQRVKEIDP
                     AIFTKSGIMVGLGEERHEVLQVMDDLRSAEVDFLTIGQYLQPTRKHHAVMRYVTPDEF
                     LGYEKVAYTKGFLMVSASPLTRSSHHAGDDFARLRAARAALSG"
     gene            1298..1768
                     /locus_tag="VGA15_22650"
     CDS             1298..1768
                     /locus_tag="VGA15_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX9590530.1"
                     /translation="MPRISSKRRILHSATQMFDLVADVERYPEFVPLCKSLNIRERTP
                     KPDGTEIVICEMTVSFKLVRETFTTRVTLDRPNLKILVEYLKGPFSNLENRWTFEQKS
                     DHACDVGFFLSYEFKSRMLALLMGTMFDTAFQRFAAAFEKRADAIYGKPANASS"
     gene            complement(1784..2386)
                     /locus_tag="VGA15_22655"
     CDS             complement(1784..2386)
                     /locus_tag="VGA15_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX9590531.1"
                     /translation="MSGSDARALSRSLLDLCRMRKLTIATAESCTGGLVAGALTDIPG
                     SSDVIDRGFVTYSNDAKRVMLGVKATTLATFGAVSKETATAMAIGALEKAGVDLAVSI
                     TGIAGPGGATPGKPVGLVHFAVAARDGRILHRECRFGAIGRSAVRLRSVVEALRMLTE
                     LARGPQLPAKPRREAASRLRLRVARSPRRNAVKRRRPPRT"
     gene            complement(2388..3614)
                     /locus_tag="VGA15_22660"
     CDS             complement(2388..3614)
                     /locus_tag="VGA15_22660"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEX9590532.1"
                     /translation="MLKPDRTAAILVAAGRGLRAGSGGPKQYRLIGGQTVIFRAMEPF
                     CRHPRVFAVQPVLNPDDAAAFNAAVSELRHEPPANGGATRQASVLAGLEALAAQKPDV
                     VLIHDAARPFVSAALISRAIDAASRTGAAVPAIQVTDTIKQVGETGNVEGTPDRARLR
                     IAQTPQAFRFDVILDAHRRAAREGRSDFTDDAALAEWAGLTVATFEGDPANMKLTTPE
                     DFIREEARLGAMLGDIRTGTGYDVHAFGDGDHLMICGVRVPHSRGFLAHSDGDVGLHA
                     LVDAILGALADGDIGSHFPPSDSKWKGAASDRFLKYAVDRVTARGGRIANLEVTLICE
                     RPKIGPLRDTMRARIAEITALDISRVAVKATTSERLGFTGREEGIAATASATIRLPWT
                     DKGWTDKGWGDKGRSD"
BASE COUNT          737 a         1220 c         1251 g          707 t
ORIGIN      
        1 accagcagcg tcgcgccgat caggtcgggc gccacctcgt gcacgctgcg ggcaaagaac
       61 gcacgcttga gcgggcggcc cagatcctgc tcatccaacg ttttgctcat tcaatgtgcg
      121 attttgagcc attccaggcg gtatgctaga gcgttttcaa gcgaagtgga gaccggttcg
      181 cgttaagaaa acgcgtcaaa tcaaagaatc tagagcctcg tttcgattct atcgaaacgg
      241 ggctctaaga cccggtgcag agcatggcga tggcggttgc gattcccggt ccagcggact
      301 agctaaaggg cttagaatgg acagtccatg gtcgtcgtcg tcgataccgt ttcagccaca
      361 ccgctgcgcc cacgtcaccc cgaaaaggtg aaccggccgg acgcattgtc gccgccgaag
      421 ccggactgga taagggtgcg cgcgccgaat acccgcggct atgccgacac ccgccagatc
      481 gtcagggaaa acggcctcgt cacggtgtgc gaggaggcgg gctgcccgaa catcggcgag
      541 tgctgggaca agaagcacgc caccttcatg atcatgggcg acacctgcac ccgtgcctgc
      601 gccttctgca acgtcaagac cggcatgccc ggggcgctcg atgcgcatga gccggaacac
      661 gtcgcggaag ccaccttcaa gctcgggctt gcccatatcg tgattacctc ggtggatcgc
      721 gacgacctcg ccgatggcgg tgcagaacat ttcgcgcgaa ccattggcgc gattcgcgcg
      781 cgctgcccga ccacgaccat cgagatcctg acgccggatt tcctgcgcaa gcagggcgcg
      841 ctcgaaaccg tggtggcggc gaagcccgat gtgttcaacc acaatctgga aacggtgccg
      901 tcgcgctatc tcacggtgcg cccgggcgcg cgctatttcc attcgatccg gctgttgcag
      961 cgggtcaagg aaatcgatcc ggcgattttc accaagtccg gcatcatggt cgggcttggg
     1021 gaggagcgtc acgaggtatt gcaggtgatg gacgacctgc gctcggcgga ggtggatttt
     1081 ctgaccatcg ggcagtatct gcagccgacc cgcaagcatc acgccgtgat gcgctatgtg
     1141 accccggacg aatttttggg ctatgagaag gtggcctata ccaagggatt tctgatggtg
     1201 tcggcgagcc cgctgacgcg ctcgtcgcat catgccggcg atgattttgc ccgactgcgg
     1261 gcggcgcgcg cagcgttatc cggctgagcg ccgggcgatg ccgagaatct ccagcaagcg
     1321 ccggattctc cacagcgcga cgcagatgtt cgatctggtc gccgacgtcg agcgttatcc
     1381 cgaattcgtg ccgctgtgca aatcgctgaa cattcgcgaa cgcacgccaa agcccgatgg
     1441 caccgagatc gtgatctgcg agatgacggt gtcgttcaag ctggtgcgcg aaaccttcac
     1501 gacccgggtg acgctggacc gtcccaacct gaaaattctg gtcgaatatc tcaaggggcc
     1561 attcagcaac ctcgagaacc gctggacgtt cgagcagaag tcggaccacg cctgcgatgt
     1621 cggatttttc ctgtcttacg aattcaagag ccggatgctg gcgctgctga tgggaaccat
     1681 gttcgatacg gcgttccagc ggtttgcggc ggcattcgaa aagcgcgccg atgcgattta
     1741 cgggaagccg gcaaatgcct catcctgagg cccatcgccg ttatcacgtc ctcggcggtc
     1801 gccgccgctt gacggcgttg cgccgtggcg agcgcgccac gcgcagacgt aaccggctgg
     1861 cggcttcgcg gcgcggcttg gccggcaact gcggtccgcg cgccagctcc gtcagcatgc
     1921 gcaaggcctc cacgacagag cgcagccgca ccgcgctgcg gccgatcgcg ccgaagcggc
     1981 attcgcgatg cagaattcgg ccgtcgcggg cggcgaccgc gaaatgcacg aggccgaccg
     2041 gcttgcccgg tgtcgcccct cccgggccgg caattccggt aatggagacg gcaagatcga
     2101 ccccggcttt ctccagcgcg ccgatcgcca tcgcggtcgc ggtctccttg ctgaccgcgc
     2161 cgaatgtcgc aagcgtcgtt gccttgaccc cgagcatcac ccgcttggcg tcgttggaat
     2221 aagtgacaaa gccgcggtcg atcacgtcag acgatccggg gatatcggtc aatgcgccgg
     2281 cgaccaggcc gccggtgcag gattccgcgg tcgcgatggt cagcttgcgc atccggcaga
     2341 gatcgagcag cgagcgggag agggcgcggg cgtcgctgcc gctcatttca gtcgctccga
     2401 cctttgtcgc cccaaccttt atcggtccaa cctttatcgg tccaaggcag gcggatggtg
     2461 gcgctcgcgg tcgcggcgat gccttcctcg cgcccggtga agccgagccg ctcactggtc
     2521 gtcgccttca ccgcaacgcg cgaaatgtcc agtgcggtga tctcggcgat ccgcgcacgc
     2581 atggtgtcgc gcaacggtcc gatcttcggg cgctcgcaga tcagcgttac ctcgaggtta
     2641 gcaatgcgcc cgccgcgcgc ggtcacgcga tcgacggcgt atttgaggaa gcggtccgac
     2701 gcggcgccct tccatttgga atcgctcggc ggaaagtgcg agccgatatc gccgtccgcc
     2761 agcgcgccga gaatggcgtc aaccagcgca tggaggccga cgtcgccgtc ggaatgggca
     2821 agaaagccgc ggctgtgcgg cacgcggacg ccgcagatca tcaggtgatc gccatcgccg
     2881 aaggcgtgca cgtcatagcc ggtgccggtc ctgatatcgc cgagcatggc gccaaggcgg
     2941 gcttcttcgc ggataaaatc ttccggtgtc gtcagtttca tgttcgcagg atcgccttca
     3001 aaagtcgcca ccgtcaatcc cgcccattcc gccagcgccg cgtcatcggt gaaatcgctg
     3061 cggccctcgc gcgcggcacg gcgatgggca tcgagaatca catcgaaccg gaacgcctgc
     3121 ggtgtttgcg cgatccgcaa ccgcgcgcga tccggagtgc cttcgacgtt tccggtttcg
     3181 ccgacctgct tgatggtgtc tgtgacctgg atcgcaggaa cggccgcgcc ggtgcgggaa
     3241 gcggcgtcga tcgcgcgcga gatcagcgcc gccgagacga acgggcgcgc cgcatcatgg
     3301 atcagaacga catccggctt ttgggcagca agcgcttcaa gtccggcgag aaccgaagcc
     3361 tggcgcgttg ccccaccatt cgcgggcggt tcgtggcgca attcgctgac ggcggcattg
     3421 aaggcagctg cgtcgtcggg attcaaaacc ggctgcacgg cgaagacccg cggatgccgg
     3481 caaaacggtt ccatggcgcg gaaaattacg gtttgcccgc cgatcaagcg atattgcttc
     3541 ggtccgcccg agccggcacg aagcccgcgt ccggctgcga caaggatggc tgcagtccga
     3601 tctggtttca gcatgggact caatactgat gatttaaggc ttgacgaggg gagcatcggt
     3661 tcgccggcgc acagccctta tcatgctggg ccgcgaaagc ggaacaattc accatggccg
     3721 ttcactatgg ctgtgggaaa agcatatctt gctgcagtgc acttgcaaga ttagcagaac
     3781 tgtctaaagt gtaggcatga agcttgactg cacaagattt gtgctcacaa tgggccccgg
     3841 cggtcgcgat ggcgcgcccg ccagaaaaac gagaaatccg tgaccggctc ggaatgttca
     3901 ggccttaagc cgttg
//
LOCUS       DASWGM010000821         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1319041,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000821 DASWGM010000000
VERSION     DASWGM010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319041"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..621
                     /locus_tag="VGA15_22665"
     CDS             445..621
                     /locus_tag="VGA15_22665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590533.1"
                     /translation="MKPNRVRVPENFLFPDGASTPDLDALKTAKARQDVSHQKLDKWI
                     AAAIAELRDEKRVA"
     gene            complement(668..>1801)
                     /locus_tag="VGA15_22670"
     CDS             complement(668..>1801)
                     /locus_tag="VGA15_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9590534.1"
                     /translation="VECVLDELAEALKMDPLELRLKNAAKQGTKAAFGPVFPRIGYIE
                     TLEAAIAHPHYKAPLGKLQGRGVASGFWFNAGGESSAQVNITEDGNVVVTTGHPDIGG
                     SRAAIANICAELLGIDYRRVSVLIGDTATVGFSNLTGGSRVLFASAMVVTQSTDKVIA
                     TLRERAAKIWEIDPEAVKWENGAAHPASPNAGQFEPLTLAELAAKAPQMGGPIGAGVQ
                     LNTEGAEGGFGTHICDVEVDPELGIARVIRYTAVQDVGRAVHPGYVEGQLQGGVAQGI
                     GWALNEEYIYNRDGKVDNPGFLDYRMPVCSDLPMIDTVLVEVPNPKHPQGVKGVGEVP
                     LVPVMAAVANAIHNALGKRFYSLPMSPPKVLEVIEAGALEAAE"
BASE COUNT          336 a          588 c          535 g          342 t
ORIGIN      
        1 agcttccagg cgcccgaatt ctataagcgt catggctacc aggtattcgg ccaaatcgac
       61 tgccatgcgg gagcggcgcg catttttcta accaaggaac tggtgtgaag gcgaatttgc
      121 gttacggcga accaggcgca aacagcccgt aaggctcgcc cacgccgacg aggtgttgct
      181 gcaatgcaac gtcacatcct gcctggcgcg ttttcaagcg aaagcccgcc ccggacttga
      241 tccggggtgg caaccggttc gcgtcaagaa aatgcgttga atcaagaagc tagagcccca
      301 tttcgattct atcgaaacgg aaaggctcca gcgccaaaat tggcgttgtc attttttctg
      361 cacatttctg ccgctatcgg cggactaaat tcaccatcgt tatgcgtgcc cgagaatttg
      421 tatccagtat ttcgggggac agaaatgaaa ccgaaccgcg ttcgcgtgcc tgaaaacttc
      481 ctgtttccgg acggcgcgtc gacgccggac ctcgacgccc tgaagacggc gaaggccaga
      541 caggacgtat cccaccaaaa actggacaag tggatcgcgg ctgcgattgc cgagctccgc
      601 gacgaaaaac gcgtcgccta gcgcgcggcc cgatgacagg ctccggcgta atccgccata
      661 cgcagcgtca ctccgccgcc tccagcgccc ctgcctcaat cacctccagt accttcggcg
      721 gtgacatcgg caaactgtaa aagcgttttc ccagtgcgtt gtggatggcg ttggcgaccg
      781 cggccatgac cggcacgagc ggcacttcgc cgaccccttt cacgccttgc ggatgtttcg
      841 ggttcggcac ttcgaccagc accgtgtcga tcatcggcag gtccgagcag accggcatgc
      901 gatagtcgag gaagccgggg ttatcgacct tgccatccct gttgtagatg tattcctcgt
      961 tcagcgccca gccgatccct tgcgcgacgc cgccctgcaa ctggccctcg acatagcccg
     1021 gatgcacggc acggccgacg tcctgcacgg cggtgtaacg gatcacgcgc gcgatcccga
     1081 gctcagggtc gacttccacg tcgcagatgt gcgtaccaaa accgccctcg gcgccttcgg
     1141 tgttgagctg cacgccggcg ccgatcggcc cgcccatctg cggggccttg gccgccagtt
     1201 cggccagcgt cagcggttcg aactggccgg cgttggggct ggccggatgc gccgcgccat
     1261 tttcccattt gaccgcctcg ggatcgatct cccagatctt tgcggcgcgc tcgcgcaagg
     1321 tggcgatgac cttgtcggtc gattgcgtca cgaccatcgc ggacgcgaac agcacgcggc
     1381 tgccgccggt caggttcgag aagccgacgg ttgcggtgtc gccgatcaaa accgacaccc
     1441 gccggtagtc gatgccgagc agctcggcgc agatgttggc gatcgcggcg cgcgagccgc
     1501 cgatatccgg atggcctgtg gtgacgacga cgttgccgtc ctcggtgatg ttgacctggg
     1561 cgctcgattc accgccggcg ttgaaccaga agccggaagc cacgcccctg ccctgtagtt
     1621 tgccgagcgg cgccttgtag tgcggatgcg caatcgctgc ctccagcgtc tcgatgtaac
     1681 cgatgcgcgg aaacaccggg ccaaacgcgg ccttggtgcc ctgcttggcg gcattcttca
     1741 accgcagttc cagcggatcc atcttcagcg cttcagccag ctcatcgagc acgcactcca
     1801 c
//
LOCUS       DASWGM010000822         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1596782,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000822 DASWGM010000000
VERSION     DASWGM010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596782"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VGA15_22675"
     CDS             complement(<1..296)
                     /locus_tag="VGA15_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9590535.1"
                     /translation="MRARRWFLSGGLCLRLIALTAAPITPASAQEDPSKYPTRPVHII
                     VGFSAGGGNDIIARIFGQKLSESLGQPVIVENKPGGGAIVATEYVAKSAPDGYTL"
     gene            complement(308..2041)
                     /locus_tag="VGA15_22680"
     CDS             complement(308..2041)
                     /locus_tag="VGA15_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX9590536.1"
                     /translation="MSRHSTAHHFLQGLVDLGVEYIFANLGTDHVSLIEEMARWDSEG
                     LKHAEMILCPHEVVAVHMAGGYALATGRGQAVLVHVDAGTANAAMAIQNLFRYRLPVM
                     LFAGRAPYTLHGELPGSRDTYVHFVQDPFDIASIVRPYVKWEYSLPSGIVVKEALARA
                     SAFAQSDPPGPVYMMLPRETLAEQWDDVDMPAYPPARYGGVHTGGIEPARVDAIARAL
                     MAAKNPIALTAYLGRKPDAVAVLERLARTCGIRVAEFNSIDLNISQDSPCFAGFDPLP
                     LLETADLGLLLDSDVPFIPQYARRAGAIKWIQIDVDPLKSDFPMWGFPTDMRIQGDCA
                     IVLQQVIDAVEARADDAWRKRVAERIASWSGAREATVKRRSAAGANKGISGALNPAFV
                     FTTLNAKLSQDDIVINEAIRNAPILQEHITRTKPSSYVGLAGGGLGFSGGMALGLKLA
                     QPERRIVQVIGDGGYHFSSPDSVYAVAQQYQIPVLTVVLDNGGWQAVKSAVQRVYPKG
                     IAAETDQFQSRLRSGRQGEQRDFSAVARAFGAHGECVTEPDDLDSAIDRCLSALGDGK
                     AAVLHVHVTPL"
BASE COUNT          396 a          667 c          666 g          397 t
ORIGIN      
        1 agcgtgtagc cgtccggcgc cgacttcgcg acatattcgg tggcgacgat cgcgccgccg
       61 cccggcttgt tttcgacaat gacgggctgg ccgaggcttt cggacaattt ttgcccaaaa
      121 atccgcgcga tgatgtcgtt gccgccgccc gcgctgaagc cgacgatgat gtgaacaggc
      181 cgggtcggat attttgatgg atcttcctgc gccgaagcgg gcgtgatcgg ggccgcggtg
      241 agcgcgatca atcggagaca gagaccgcct gacagaaacc agcgacgggc cctcatcggc
      301 ttgttcctta gagcggcgtg acgtgaacgt gcagcacggc ggccttgccg tcgccgagcg
      361 cagacaggca gcggtcgatg gcggaatcaa gatcgtcggg ttcggtgacg cattcgccat
      421 gggcgccgaa tgcccgcgcg acggccgaaa aatcccgctg ctcgccctgg cggcccgatc
      481 tcagccgcga ctgaaactga tcggtttcgg cggcaattcc cttgggatag acccgctgca
      541 cggcggattt caccgcctgc cagccgccat tgtcgagtac caccgtcaga accggaatct
      601 gatattgctg cgccactgcg tagacgctgt cgggcgatga gaaatgatat ccgccgtcgc
      661 cgatcacctg cacaatgcgg cgctccggct gcgccagttt taatcccagc gccatcccgc
      721 ccgagaatcc aagcccgccg ccggcgaggc cgacatagct cgaaggcttg gtgcgcgtga
      781 tatgctcctg caggatcggc gcgttgcgga tcgcttcgtt gatgacgata tcatcctgcg
      841 acagttttgc gttcagcgtg gtgaacacga aggccgggtt gagcgcgccc gaaatgcctt
      901 tgttggcgcc cgcggcggaa cggcgtttta cggttgcttc gcgcgcaccg ctccagcttg
      961 cgatgcgctc ggctacgcgt ttgcgccagg cgtcgtcggc gcgcgcctcc acggcgtcga
     1021 tcacctgctg cagcacgatt gcgcaatcgc cctggattcg catgtcggtc ggaaatcccc
     1081 acatcggaaa atccgacttc aagggatcga catcgatctg gatccatttg atcgcgccgg
     1141 cacgccgggc gtattgcggg atgaacggta catccgaatc caagagcagg ccgagatcgg
     1201 cggtctcaag caggggcagg ggatcgaagc cggcgaaaca gggcgaatct tgcgagatat
     1261 tgaggtcgat cgaattgaat tccgcgaccc ggatgccgca ggttcgcgcc agcctctcca
     1321 gcaccgccac ggcgtccggc ttgcggccga gataggcggt cagcgcaatc ggatttttcg
     1381 ccgccatcaa tgctcgtgcg atcgcatcga cccgcgcggg ctcgataccg cctgtgtgca
     1441 cgccgccata tcgtgctggc ggataggccg gcatgtcgac gtcgtcccat tgttcggcga
     1501 gcgtttcgcg cggcagcatc atgtaaacag ggccgggcgg atcgctttgc gcaaacgcgc
     1561 tggctcgggc caaggcttcc ttcaccacga tgccggaggg cagcgaatat tcccatttga
     1621 catagggcct gacgatgctg gcgatgtcga acgggtcctg cacgaaatgc acataggtat
     1681 cgcgcgagcc cggcagttcg ccgtgcaacg tatacggcgc gcgaccggca aacagcatca
     1741 ccggcaagcg atagcgaaac aggttctgta tcgccatggc ggcattcgcc gtgccggcat
     1801 cgacatgcac cagaaccgcc tggccgcgtc ccgtcgccaa ggcatagccg ccggccatgt
     1861 gcaccgcgac gacctcgtgc gggcacagga tcatttcggc atgcttcaag ccttcactgt
     1921 cccagcgcgc catctcctcg atcagggaga cgtggtcagt tcccagatta gcaaagatgt
     1981 actccacgcc gagatcgacc aggccttgga ggaaatgatg cgcggttgag tgacggctca
     2041 tgctctatga ctttcccggc agcgtctccg cgacaaactt gttgcgcagc gtaccgatgc
     2101 cggtgatgtc gatctcgacc acgtcg
//
LOCUS       DASWGM010000823         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_498941,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000823 DASWGM010000000
VERSION     DASWGM010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498941"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..825)
                     /locus_tag="VGA15_22685"
     CDS             complement(22..825)
                     /locus_tag="VGA15_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9590537.1"
                     /translation="MRSFLVAIAGLMLFTAGAAQAKIDITIDKNAQLMTVAVDGAVRY
                     QWPVSTGIPSRETPNGSFRAFRMEEDHFSKEFDDAPMPHSIFFTKIGHAIHGTDSEGR
                     LGTPASHGCVRLSKANAATLYAMVETDGVLNTTVTLTGSSQIALARNPRTRANTAVAG
                     RDPDQPYAPQYNGAGDPVMLSPLPPPRQIAPVAQPPAQADDGYIYPADGNSDVARYPA
                     PRSSRRLYDAQANPPQQYYDNRGYAPQQAPQVYAPRPYYQPRRLFTYQD"
     gene            1128..1448
                     /locus_tag="VGA15_22690"
     CDS             1128..1448
                     /locus_tag="VGA15_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590538.1"
                     /translation="MMKMKVKTAPLPVGLPVRLYLAVAAIVLAWLAIPHIGHAQGIVR
                     GAQEGSYEGNRVAGPIGGVVGGAVGAGVGGAMGAVKGVFGISDRGYRCRGYYDRHDRF
                     HCYR"
     gene            complement(1586..>2123)
                     /locus_tag="VGA15_22695"
     CDS             complement(1586..>2123)
                     /locus_tag="VGA15_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590539.1"
                     /translation="ASFGIYFPKFVGTIYELLSAPISYFEIVLGYVGAAATKSIILGL
                     IILATAGLFVPLQIHHPWWMLTFLVLTAVTFSLFGFIIGIWADGFEKLQMIPMLVVTP
                     LTFLGGSFYSVNMLPSGWRTITLLNPVVYLISGFRWSFYEISDVTVGLSVGITLMFLA
                     ICMVTVWWIFKTGYRLKN"
BASE COUNT          391 a          614 c          719 g          399 t
ORIGIN      
        1 gcgggataac gcgagcgctt cctagtcctg atacgtgaac agccggcgcg gctggtaata
       61 tggccgcggc gcgtagacct gcggcgcctg ttgcggtgcg tatccgcggt tatcgtaata
      121 ttgctgcggt ggattggcct gcgcgtcgta aaggcgccgg gaactgcgcg gcgcgggata
      181 gcgcgccacg tccgagttgc cgtcggccgg atagatgtag ccgtcgtcag cctgcgcggg
      241 tggctgcgcg accggcgcga tctggcgcgg cggcggcagc ggagacagca tcaccggatc
      301 gccggcgcca ttatactgcg gagcgtatgg ctgatccggg tcgcggccgg cgacggcggt
      361 attggcgcgg gtccgcgggt tgcgcgccag tgcgatctgg gaagagccgg tgagcgtcac
      421 ggtggtgttg agcacgccat ctgtctcgac catcgcatac agcgtggcag cgttggcctt
      481 cgacaggcgt acgcagccat gggacgcggg cgtgccgagg cggccttcgg agtccgtgcc
      541 gtggatggcg tggccgatct tggtgaagaa gatcgagtgc ggcatcggcg cgtcgtcgaa
      601 ttccttggag aagtggtctt cctccatgcg gaaggcgcgg aagctgccgt ttggcgtctc
      661 gcgcgacgga atgccggtcg ataccggcca ctgatagcgc acggcgccgt cgaccgcgac
      721 ggtcatcagc tgcgcattct tgtcgatggt gatgtcgatt ttggcctgcg cggcgccggc
      781 ggtgaacaac atcaggccgg cgatagcaac gaggaaggaa cgcattttac ctttggcctc
      841 caggccttca ctgcgacgcc ttgactgcaa cgccgctctc accgtaccgc atcaatatgc
      901 cccgaattcc gctttggttc cagcggtccg tcggtcaatc gttaatgtct ggcagggcgc
      961 aaacaaggcg gaggccgggc tgaatgcggc gggccgcgca atttttcggg aggcgccgcc
     1021 ttgtcgtgct gtcctggatt taccacagac gcgcaacctt ttggccgtat cggcgttaca
     1081 agtttccgcc gcgccggtcg gttgcggtcg agccccaagg aattcagatg atgaagatga
     1141 aggtcaagac agcgccgctg ccggtagggt tgcccgtaag actctatctt gcggtcgcgg
     1201 ccattgtgct ggcatggctc gcgattcccc atatcgggca cgcccagggc atcgtgcgcg
     1261 gcgcgcagga aggttcctac gagggcaacc gggtcgccgg cccgatcggt ggtgtggtcg
     1321 gcggcgcggt cggagcaggt gtcggcggcg cgatgggggc cgtgaagggc gtgttcggca
     1381 tctcggaccg cggctatcgc tgccgcggct actacgaccg ccacgatcgc ttccactgct
     1441 atcgatagtt tttgtttgta acgtcattcc ggggcgcgct ttagcgcgag cctcagatgt
     1501 gcaattgcac atcgaggaaa ctcaagattc cgggttcggc gcttcacgcc gccctggaat
     1561 gacattttag ctattgccct tcgcgtcagt tcttcagccg atagccggtc ttgaaaatcc
     1621 accacaccgt gaccatgcag atggcgagaa acatcagcgt gatgcccaca ctcaggccca
     1681 cggtgacgtc ggagatttcg taaaagctcc agcggaagcc gctgatcaga tagaccaccg
     1741 gattgagcag cgtgatggtg cgccaacccg aggggagcat gttcaccgaa tagaagctgc
     1801 cgccgaggaa cgtcagcggc gtgacgacca gcatcgggat catctgcagc ttctcgaaac
     1861 cgtcggccca gattccgatg atgaagccga acaggctgaa ggtcaccgcg gtcagcacca
     1921 ggaacgtcag catccaccag ggatgatgaa tctgcagcgg cacgaacagg ccggcggtgg
     1981 cgagaatgat caggccgagg atgatcgatt tggtcgcggc cgcgccgaca tagccgagca
     2041 cgatctcgaa atacgagatc ggcgccgaca gcaattcgta gatcgtgccg acgaatttcg
     2101 gaaagtagat gccgaacgag gca
//
LOCUS       DASWGM010000824        10047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1054302,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000824 DASWGM010000000
VERSION     DASWGM010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054302"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VGA15_22700"
     CDS             complement(<1..611)
                     /locus_tag="VGA15_22700"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9590540.1"
                     /translation="MRSACTWPCTWPLVVAGRSVTALALVFALSALVTGSARADDYPN
                     RPVKIIVPFAAGGTADAIPRLVGDWLSRKWGQPVVIENRTGAAGNIGADFVYHSDPDG
                     YTLLSSPPPPLVINQNLYPKLGFDPAKFEPVIVMAQVPNALIVNPKNLKASSVSELID
                     YLQQNPEKVTAATQGIGTTSHLTAELFQVMAKVKLRTIPYRGSA"
     gene            736..1917
                     /locus_tag="VGA15_22705"
     CDS             736..1917
                     /locus_tag="VGA15_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9590541.1"
                     /translation="MISRPHVAIIGGGIGGLFAANALAAQGIRISVYEQAPAIGEIGA
                     GVFLTPNSVRHLQRMGLEPAVEKWGARVGPDSRYFRHDGAPIAPVQVTDSSGWNATFG
                     MHRADLVAMLADALPAGTVHTGHRCCGFEQDGNTACVSFANGVSVEADVVIAADGIHS
                     ELRSYVVPSSRPVFSGSVAYRGLVPHERVPHWPTDRWQMWLGKGRHFLAFPVRAGKLI
                     NYVGFMPTDEAMKESWSAPGDPEVLREAFAGWDPRINELLAQVQVTFRWALYDREPLP
                     LWTRERLSLLGDAAHPMLPHLGQGANQSIEDGMALATILKDADRKTAPKALLAYERLR
                     RERVAQVQRGARENGMRYDSAYSDLGVRDAEITAHAAFRKRLYDHDVVPDAQAAAATL
                     I"
     gene            2024..2878
                     /locus_tag="VGA15_22710"
     CDS             2024..2878
                     /locus_tag="VGA15_22710"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590542.1"
                     /translation="MTRISIVTGATRNLGFSLAQGLARRLEPDDVVYLTGRDPGRVAE
                     SVRLLSGHRANVTGEVLDVSDREAVERFANALADRHGGVDIVFSNHYARVQPGENAAE
                     VIDGYVAANNLGTTHVLRSFAPLIRDNGRLLVVASRAGSLRALAPALHGRFENLQTLD
                     DVDRAVCAWRDVVRQGLAAGQAWPEWINIPSKIGQVAAVRVLAAQRRAEDMRRGILIA
                     AISPGLIDTGASRQWLDMTGAPPPEEVAIPLLDLALEEAPDSALYGGLIHLGHGEPGR
                     FRTLVPWR"
     gene            2835..3335
                     /locus_tag="VGA15_22715"
     CDS             2835..3335
                     /locus_tag="VGA15_22715"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9590543.1"
                     /translation="MESLGGFGHLCLGDETLEMKQRKKVGADERSVGMLVKRAEQAML
                     KSKTAALKPVGLTLAQYVALAELEQKQGITAATLARACFVSPQAIMILLKSMEQQGLI
                     TRRPHPRHPNVLELHVTEVGREAFHAGRAQVAPVEESIFAAFSKQELEAFRGFLSRFV
                     SAIDAG"
     gene            complement(3716..3937)
                     /locus_tag="VGA15_22720"
     CDS             complement(3716..3937)
                     /locus_tag="VGA15_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590544.1"
                     /translation="MTAVIDMIEHRPVRVFPWIDGGAVLGGVTFGRHGAARAMATARR
                     ESAGRGWGYQADDVRNSGAGIFEREDIDR"
     gene            4049..5347
                     /locus_tag="VGA15_22725"
     CDS             4049..5347
                     /locus_tag="VGA15_22725"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX9590545.1"
                     /translation="MFTRVLALVSGALLGLATSNALAQGLPAAKPEEIGLSAAKLQRL
                     TDAFQAEIDKGAIPGAVLLVARNGKVGYFKAIGFQDRDKQIAMKPDSIFWIASLTKPI
                     ATVAAMMLVEEGKIQIDDPVARYLPELSSMQVGVEKINASTGKPELVLEPARRPMTVQ
                     DLLRHTSGFTYPSFGNSLVNQAYGAANVFDISLTLGEFVTKLSKLPLSYQPGTTWDYG
                     VSTDVLGRIVEVVSSMPLDQFIAERITQPLHLADTAFVVPQAKGSRIAQPQNEPTTGK
                     PPAFYHDMSVPPKLFSGGVGLVSTASDYARFCQMLLNGGRLDGTRLLEARTVRYMTVD
                     HLPPDVGFSPAAYFFGASGPTRAQGQGFGLGFAVRNDEGRNARFGNPGKYFWISVTGA
                     AFFVDPQEKLIGVMMVQLPIIQTQHYRSLFENLVYQAVDK"
     gene            complement(5401..5718)
                     /locus_tag="VGA15_22730"
     CDS             complement(5401..5718)
                     /locus_tag="VGA15_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9590546.1"
                     /translation="MTARPDRYCPIARTLDIVGERWTFLILRDLVLDGPRKFQDFEKS
                     LSGISPNTLSARLKTLEDNGIVERRFYSDHPPRAEYVITAKGMELRPVLRTLKAWGEK
                     HIL"
     gene            5929..6441
                     /locus_tag="VGA15_22735"
     CDS             5929..6441
                     /locus_tag="VGA15_22735"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX9590547.1"
                     /translation="MTICGNNAVRAALGALALAASCTLASAQGAGHSQQPGAALVERY
                     VLAFNAHDADAFRDVIANDYVQHNGRAGQGLAGTQATLRGYFQTFPDMHVQVEDLIID
                     GDKVVARLTITATHNHPVQLGPNAPIFQPTGKRLAWGDIEIWRVADGKFVEHWDQSDL
                     AGLARQMRPD"
     gene            complement(6458..7506)
                     /locus_tag="VGA15_22740"
                     /pseudo
     CDS             complement(6458..7506)
                     /locus_tag="VGA15_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(7521..7709)
                     /locus_tag="VGA15_22745"
     CDS             complement(7521..7709)
                     /locus_tag="VGA15_22745"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9590548.1"
                     /translation="MTLVRANPEALVWGGDWPHPRVEGEMPDAGHLFELFQQWTPDRA
                     TQRRILVTNPAKLYGFPN"
     gene            complement(7706..8674)
                     /locus_tag="VGA15_22750"
     CDS             complement(7706..8674)
                     /locus_tag="VGA15_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9590549.1"
                     /translation="MGGWFRMIAAAVAMVAAGGVASAQSSFPSKPVHIFVPYAAGGGV
                     DILARTLGDVVSRQWGQTVVVENRPGAGGVVASQALVASPPDGYTLIVVASGHATNPF
                     LYPKIPYDTFKDFAPISLLASSPNILLVRADSPFKVLGDVIAQARAKPGSLSFGHAGT
                     GTSTHLAGELLKNLARIDLNAIPYKGGAPSINDLLGGQIPMSFNNGPESVGQLEAGTV
                     RALAVTTASRAPFLPDVPSMAETVPGYDTEVWWGLLGPAGMPPDLVAKLSHDFVAALN
                     TEAVKERLAKLGAAPIGSSPEQFDARIHADYKKWGPIIEAAGMKAE"
     gene            8872..9459
                     /locus_tag="VGA15_22755"
     CDS             8872..9459
                     /locus_tag="VGA15_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HEX9590550.1"
                     /translation="MAAAEGQISPQAFQDKAFQDQAPRERSWHEIVLQTLKRNEIRLV
                     PYVPDRVLTTLIKNIHADPFFTTFPTAREEEAVGIVAGAWMGGLRGAVLMQTSGFATL
                     ANVLASLAIPYQIPLIMFVSERGTLGEFNYGQSLVCRTMRPVLNSLAMEHHTATRLDE
                     FEFIVDRSIKQAITTQAPVALILSPLLTGGKVFEK"
     gene            9476..>10047
                     /locus_tag="VGA15_22760"
     CDS             9476..>10047
                     /locus_tag="VGA15_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9590551.1"
                     /translation="MATNPPARNIKVMNRFDLTSRLVAKLKNQEAVIGGIGNTNFDLW
                     AAGHRPQNFYMLGSMGLAFPIALGVALAQPERRVFALEGDGSLLMQMGCLATIATQAP
                     KNLTMVVMDNGVYQITGAQPTPAAAVADIVAIAAGCGLSNSAWAADEEDFERLVDQSL
                     SATAPTLIAVRIDDKPGVGATRRDPVQIRER"
BASE COUNT         1914 a         3091 c         3142 g         1900 t
ORIGIN      
        1 gccgaccctc gatacgggat ggtgcgcagt ttcaccttgg ccatcacctg gaacagttcg
       61 gccgtcagat gcgaggtcgt gccgatgccc tgcgtggcgg ccgtgacctt ctcggggttc
      121 tgttgcaggt aatcgatgag ctctgacacg ctcgatgcct tgaggttttt tggattgacg
      181 atcagcgcgt tcggaacctg ggccatcacg atgacgggct cgaatttcgc cggatcaaat
      241 ccgagcttcg gatagagatt ctggttgatc acaagcggcg gcggcggtga cgaaagcaac
      301 gtgtagccgt cggggtccga atgatagaca aaatcggcac cgatgtttcc agccgccccg
      361 gtacggttct cgataacgac gggctggccc catttgcgcg agagccaatc cccgaccagc
      421 cgcgggatgg catcggccgt cccgccggcg gcgaagggca cgatgatttt tacgggacgg
      481 ttcggatagt cgtcggcacg cgccgaaccg gtgacgagcg ccgagagcgc gaacacgagc
      541 gccagcgcgg tcacggatcg gccggcaaca accaatggcc aggtgcatgg ccaagtgcat
      601 gcgctacgca tcagatgccc ttcccccaat atcccggcgg gtttgaccgc acgcgatggc
      661 aaaatgcctt ggtctgtgtg aggatacaat gccgtagcca attttgctcg cgcgttcagt
      721 gacaaggagt tgatcatgat ttcccgtccc catgtcgcga tcattggtgg cgggatcggg
      781 gggttattcg cggccaatgc gctggccgcg caaggtatcc ggatttcggt ctacgagcag
      841 gcgcccgcga tcggcgagat cggggctggc gtgtttctca cccccaacag cgtacgccat
      901 ctgcaaagaa tgggattgga accggcggtc gagaaatggg gagcgcgtgt cggccccgat
      961 tcccgctact tccgccacga tggcgcgccc atcgcgccgg tccaagtgac ggattcctca
     1021 ggctggaacg cgacgttcgg gatgcatcgc gccgacctcg tcgcgatgtt ggccgatgcg
     1081 ctgccggccg gaacggtgca taccggacat cggtgctgcg ggttcgagca ggacggcaac
     1141 accgcctgcg tgtcgttcgc caatggcgtc tcggtcgaag ccgatgttgt catcgctgcc
     1201 gacggcatcc attccgagct gcggtcttat gttgttccgt cgtcgcggcc ggtgttctcg
     1261 ggatcggtcg cgtaccgggg tctggtgccg catgagcgcg taccgcattg gccgaccgac
     1321 cgctggcaga tgtggctcgg caaaggaagg cattttcttg cctttcccgt ccgcgccgga
     1381 aagctgatca actatgtcgg gttcatgccg acggacgagg cgatgaagga atcctggtcg
     1441 gcccccggag accccgaggt tcttcgggag gcgtttgcag gatgggaccc gcgcatcaat
     1501 gaactattgg cgcaagttca ggtgaccttc cgatgggcgc tgtatgatcg cgagccgctg
     1561 ccgctctgga ccagggaaag gctgagcctg ctcggcgacg ccgcgcatcc gatgctgccg
     1621 catctcggac aaggcgccaa tcaatcgatc gaagatggca tggcgctcgc cacgatcctg
     1681 aaggatgcgg accgcaagac ggccccgaag gcgctgctgg cgtacgaacg gctccgccgc
     1741 gagcgggtgg cgcaagtgca gcgcggcgcg cgcgagaacg ggatgcgata cgactccgcc
     1801 tattccgacc tcggtgtcag agacgccgag atcaccgcgc acgccgcgtt ccgcaagcgg
     1861 ctctacgatc acgacgtcgt ccccgacgcg caagccgcgg cggcaacgct gatttagctg
     1921 gttgggtctt gcagggccct ggtgatgtgc cggcctgtgc taaatcggcg cttgattgtt
     1981 atcaacatgt tgacaacaac aatatcattg atatcattcc cgcatgacaa ggatttcgat
     2041 cgtgacgggc gcgacccgca atctcggctt cagcctcgcc caggggcttg ctcgccggct
     2101 ggaaccggac gacgtggttt atctgacggg gcgagacccg ggccgcgtcg ccgaatcagt
     2161 ccgtcttctg tccggtcatc gcgcgaacgt aacaggggaa gttctcgacg tgtcggaccg
     2221 tgaggcggtg gagcgttttg caaatgctct cgccgaccgt catggtggcg tcgatatcgt
     2281 attctcgaac cactacgcac gggtgcagcc gggcgagaat gccgctgaag ttatcgacgg
     2341 ctatgttgcc gccaacaacc tcggaaccac ccatgtcttg cggagttttg cgccgcttat
     2401 tcgcgataac gggcggctat tggtggtggc gagccgcgca ggcagcctgc gcgccctagc
     2461 ccccgcgctg cacgggcgtt tcgagaatct tcaaaccctt gatgacgttg accgtgcggt
     2521 gtgcgcgtgg cgcgatgtcg ttcgtcaggg acttgcggcc ggacaggcct ggccggagtg
     2581 gatcaatatc ccctcgaaga tcggccaagt cgccgccgtt cgcgtgctgg cggcgcaacg
     2641 gcgggcggag gatatgcggc ggggaatcct gatcgccgcg atctcgcccg ggctgatcga
     2701 caccggcgcg tcgcggcaat ggctcgatat gaccggtgct ccgccacctg aagaagttgc
     2761 gatcccgctg ttggatcttg cgttggagga agcgccggac agcgccctct atggcggcct
     2821 gatccacctc ggacatggag agcctgggcg gtttcggaca cttgtgcctt ggagatgaaa
     2881 ccctggagat gaaacaacgc aagaaagttg gagcggacga gcgaagcgtc ggcatgcttg
     2941 tcaagcgtgc tgaacaggcg atgctcaagt ccaagaccgc ggcgctcaag cctgtggggc
     3001 tcacgcttgc gcaatacgtt gcgctggcgg aactcgaaca gaaacaaggc atcacggcag
     3061 cgacgctcgc gcgggcctgc tttgtgagtc ctcaggccat catgattctg cttaaaagca
     3121 tggagcagca ggggctaatc acgcggcggc ctcatccaag gcatccgaat gtgctcgaac
     3181 ttcacgtgac cgaagtcggc cgcgaggcgt ttcacgccgg tcgcgcccaa gtcgctccgg
     3241 tggaggagag catcttcgcg gccttctcca agcaggagct ggaggccttc cgcggtttcc
     3301 tttcccgctt cgtcagcgcg atcgatgcgg gatagagatg cgggacagac tcgccagcct
     3361 tggtggagtt gaaaacgtaa cttgccgtat gtcgcggcct cgcggcggac ccgagcgata
     3421 gacggaggtc gcgtgaaaac gcctaggcgc cggccgaagg caggctggcc gccgcaatgg
     3481 cgacgatcag cgccagccgg cgtccaatcc gggacggcgc cgagcgcaac aatgaacgca
     3541 aatgtcgcat atcgcagctt ccttctgcgc cggcggaaac aatcgtttcg cgaagcaatg
     3601 cgcagaacca gaacatggtt ccgggctcta cgggtgtcga gagcgcggcg aaaatagcaa
     3661 cgcgtcgttg gtccagccgc tgtttgacag tggatttatt gccgccatcc agccgtcaac
     3721 ggtcgatatc ttctctctcg aaaatccccg cgccggaatt gcggacgtca tccgcctgat
     3781 agccccagcc tcgaccggcg gattcgcggc gcgccgttgc catcgcgcga gccgcgccat
     3841 gccgtccgaa cgtaacgccg ccaagcaccg cgccgccgtc gatccaggga aacacccgga
     3901 ccggcctgtg ctcgatcata tcgatgaccg cggtcattcg ctctccttcg atcatatcgc
     3961 cgtgtgattg aatgctaata caatagttac ttttaaaatt atagtttatg gtcgacgtcg
     4021 cataggaacg atttgacggg gatatccaat gttcactcgc gtccttgccc tggtgagcgg
     4081 tgccctgctt ggactggcga catccaacgc actcgcgcaa gggttaccag cggcaaagcc
     4141 cgaggagatc ggtctgtccg ccgcaaaatt gcagcggctg accgacgcct ttcaggccga
     4201 aatcgacaag ggagcgatcc ccggcgcggt gctgctggtc gcgcgcaacg gcaaggttgg
     4261 ctacttcaag gcgatcggct ttcaggatcg cgacaagcag attgcgatga agccggactc
     4321 gatcttctgg atcgcgtccc tgaccaagcc gatcgcgacg gtagcggcca tgatgctggt
     4381 cgaggaaggc aagatccaga tcgacgatcc agttgcgcgc taccttccgg agttatcgag
     4441 catgcaggtc ggtgtcgaga agatcaacgc ctcgaccggc aagccggaac tcgtgctcga
     4501 gccagcgcgc cggccgatga cggtgcagga tttgctgcgc cacacctccg gatttaccta
     4561 tccgtccttt ggcaactcgc tggtcaacca ggcctatggc gcggcgaacg tcttcgatat
     4621 cagcctgacg ctcggcgagt ttgtcaccaa gctgtcgaag ctgccgttat cgtaccagcc
     4681 gggcaccacg tgggattacg gggtctccac cgacgtgctt ggccgaattg tcgaggtcgt
     4741 atccagcatg ccactcgatc agttcattgc cgaacgaatt acccagccgc tgcatctggc
     4801 ggacaccgcg ttcgtcgtgc cgcaagccaa gggatcgcgc atcgcgcagc ctcagaatga
     4861 gccgaccacc ggcaagcctc cggcgtttta tcatgacatg agcgtgcccc cgaaactgtt
     4921 ctccggcggc gtcggcctgg tctcgacggc ttccgattat gcgcggttct gccagatgct
     4981 cctcaacggc ggcaggcttg acggtacgcg tctgctggag gcacgcaccg tcaggtacat
     5041 gaccgtggat catctgccgc cggatgtcgg tttcagtccg gcggcctatt tcttcggcgc
     5101 atcgggtccg acgcgcgcgc aaggccaggg ttttgggctg ggctttgccg tgcgcaacga
     5161 cgagggacgc aacgccaggt tcggcaatcc cggcaaatat ttctggatca gcgtgaccgg
     5221 cgcggcgttt tttgtcgatc cgcaggaaaa gctgattggc gtcatgatgg ttcagttgcc
     5281 gatcatccag acgcagcact accgttccct gttcgaaaac ctcgtttatc aggcggtcga
     5341 taaatgatcg aatagcgggc gtcgtccggc cggacgttgc agccgcgaat gtcggcgccg
     5401 ttagagaata tgtttctcgc cccatgcctt cagcgttcga agcacgggac gaagctccat
     5461 tcctttggcg gtgatcacat attccgctct tggcggatga tcggaataga aacgccgttc
     5521 gacgatgccg ttgtcttcaa gcgtcttcag acgagccgaa agcgtattgg ggctgattcc
     5581 ggacagtgac ttctcgaagt cctgaaactt ccgcgggcca tcgagcacga gatcgcgcag
     5641 gatcaggaac gtccagcgct cgccgacaat gtcgagggtt cgcgcgatcg gacaatagcg
     5701 gtcggggcgc gcagtcatcg acatatcctt tgatctttgg agattcagga cgagcggtga
     5761 cccaggcgcc tgaaggatcg tcgcccaaca tataacttca aaaatcatag tcaatatgga
     5821 tgacaatatc attttacata gttactatct ctttcgcagt taaatgacac gccgattcga
     5881 actcaaccag acttcattcg acgcgttcaa tcacggtgga gacgttcaat gacgatttgt
     5941 ggaaacaatg cggtccgggc agcgcttgga gctctcgcgc tggcggcgtc gtgcacgctc
     6001 gcatctgctc aaggcgctgg ccacagccag caacccggcg cagcgctggt cgaacggtac
     6061 gtactagcct tcaacgccca cgacgcggat gcttttcggg atgtcatcgc gaacgactat
     6121 gtccagcata acggccgcgc cggacagggg cttgcgggca cccaggcgac gcttcgcgga
     6181 tatttccaga ccttccccga tatgcatgtg caggtcgagg acctgattat cgatggcgac
     6241 aaggtggtgg cacgcctcac cattacggcc acgcacaacc acccggttca gctgggcccg
     6301 aatgccccga tattccagcc caccggcaaa agactcgcct ggggcgatat cgaaatctgg
     6361 cgtgtcgccg atggcaagtt cgtcgagcac tgggatcaat ccgatctggc cggtctggcg
     6421 cggcaaatgc gccctgacta ggcgtcttcc ggagccccta ccccggcgca aatccgaacg
     6481 tccgctcgaa acgtctggcg tagtccggcc agacctcggg attgatgagg cgcggcggcc
     6541 gcctgccgtc gagcgcatcg agaatttgct cggcggcgat cctgcccata ttttcccgcg
     6601 cttccctggt caccccggcc gtatgcgggc tcgccagcac gttatcgaac tgcagcaacg
     6661 gatggtccgc cggcggcggt tccttggccc agacgtcgag gcctgctcca gcgatcgatt
     6721 tgttgcgcaa tgcttcaagc agtgctgctt catcatggat aaagccgcgc gccgtggtga
     6781 tgaaataggc atgcggctgc atcagcgcga attcgcgtac gccgatcatg ccgcggctct
     6841 ccttggtcag cggacaggag atcgagacgt agtccgagcg gcgcagcagt tcctccagtt
     6901 cgaccttggc gccaccccgc tccgccatct cggtcgtcga gagataggga tcataagcca
     6961 gcacattcat gccgagcagg cccttgcaaa gggcggcgat gcggcgaccg acattgccga
     7021 gcccgacgat gccgatggtc ttgcccctgc ctcggtgccg atcaaggcgt tgcgattgac
     7081 gttggggtcg cgccgcagcg cgcgatcggc ctcgatgatg cgtttcgaca aggtcagcag
     7141 catcccgagc gcatgttcgg ccaccgaatg ggcattgccg ccggactggt tgacgacgac
     7201 gacgccggct tccgtgcagg cctcgacatc gaccggatcg tagcccgcgc cattgctgga
     7261 gacgatcaag aggttcggcg cgcgccgcag caggtcgcgg tcgacgtgga agcggggcgc
     7321 gagttcgtcg cgcgccgcac cgatttgata ggcgtgcgcc gcggccagga tcggcgccga
     7381 gatctcgtca gggctttcgt tctcgagccg gtccagccgc acatcgggcc gcgcccgcag
     7441 gatgtcggca tagatctcat gcgccagata cttgacgtaa aacacacgct tgttgttgac
     7501 ggacattcta ttgcgagcct tcagttcgga aatccataga gttttgcggg attggtcacg
     7561 aggatgcgcc gctgcgttgc ccggtccggc gtccattgtt gaaacagttc gaacaggtgt
     7621 ccggcgtcgg gcatctctcc ctcgacgcgc ggatgcggcc agtcgccgcc ccataccagc
     7681 gcttccggat tggcccgcac cagagtcatt cggccttcat gccggcggcc tcgatgatcg
     7741 gcccccactt cttgtaatca gcgtgaatcc tggcgtcgaa ttgctccgga gaactgccga
     7801 tcggcgccgc gccgagcttg gccagtcgtt ccttgacggc ctccgtgttc aaggccgcga
     7861 caaaatcatg cgagagtttc gcgacgagat cggggggcat gccggccggt ccgagcaaac
     7921 cccaccacac ttcggtatcg tagcccggta cggtctcggc catgctgggc acatcgggca
     7981 gaaacggcgc gcgcgatgcg gtggtgacgg cgagcgcgcg gacggtaccg gcttcaagtt
     8041 gtccgacgga ttccggaccg ttgttgaagg acatcgggat ctgcccgccc aacagatcgt
     8101 tgatcgaggg cgcgccgccc ttgtagggaa tcgcgttgag atcgatcctg gcaagattct
     8161 tcaaaagctc gccggccaga tgggtcgatg ttcccgtccc cgcatggccg aaggacaggc
     8221 ttcccggctt ggcgcgcgcc tgcgcgatca catcgcccag caccttgaac ggagaatcgg
     8281 cgcgcaccag caggatattg ggagacgagg ccaatagcga gatcggcgca aaatccttga
     8341 aggtgtcgta gggtattttg gggtacagga acggattggt ggcgtgaccg ctcgcgacaa
     8401 caatgagggt gtatccgtcc ggcggcgacg ccaccagcgc ctgcgacgcg accacgccgc
     8461 ccgcacccgg ccgattttcg acaacgacag tctggcccca ttgccgggag accacatcgc
     8521 ccagcgtgcg cgccaggata tccacgccgc cgccggccgc atatggcaca aaaatatgca
     8581 cgggcttgga cgggaatgac gattgagccg acgccacgcc gccagcggca accatcgcca
     8641 ctgctgcggc aatcatccgg aaccacccgc ccatggcatc cctctcccga tatcttcgtt
     8701 acgtctttta tcgcacgccg gcacagcgag ggcatcaccg tgaggcgcag atcacgggta
     8761 aagtctggac atgttgacaa tccctgagat cccgtcaagt ttcgacgcct cgacgagccg
     8821 gtcgcgatca tgtcgccaac gacaaatcgt cgcaaagcag gggagaattc gatggcggct
     8881 gccgaaggac aaatctcgcc ccaggccttt caagacaagg cctttcaaga ccaggccccg
     8941 cgagaacgga gctggcacga aatcgtgctg cagacgctca agcgcaacga gatcaggctt
     9001 gtgccttatg ttccggatcg cgtgctgacg acgctgatca agaatatcca cgccgatccg
     9061 ttcttcacca ccttccccac cgcgcgcgag gaagaagccg tcggcatcgt cgcgggtgcc
     9121 tggatgggcg gcctgcgcgg cgcggtgctg atgcagacga gcggctttgc cacgctggcc
     9181 aatgtgctgg cctcgctggc gatcccctat cagataccgc tgatcatgtt cgtgtccgaa
     9241 cgcggcacgc tcggcgaatt caattatggg cagtcgctgg tgtgccgcac catgcggccg
     9301 gtgctcaatt cgctggcgat ggagcaccac actgccacgc ggctcgacga attcgagttc
     9361 atcgtcgatc gatcgatcaa acaggccatc accacgcaag cgccggtggc gctgatcctg
     9421 tcgccgcttt tgaccggcgg caaagtcttc gagaagtgag tgaaagcgca tgaacatggc
     9481 aaccaacccc cctgcccgca acatcaaggt gatgaaccgg ttcgatctca cctcgcggct
     9541 ggtcgcgaag ctgaagaacc aagaagccgt gatcggcggc atcggcaaca ccaatttcga
     9601 tctgtgggca gccggccacc ggccgcagaa tttctacatg ctgggcagca tgggcctcgc
     9661 cttcccgatt gcgctcgggg tggcgctggc gcagccggag cgccgcgttt tcgcgctgga
     9721 aggcgacggc tcgctgctga tgcagatggg ctgcctcgcg accatcgcaa cgcaggcgcc
     9781 gaaaaacctc accatggtcg tgatggacaa cggcgtctac cagatcaccg gggcgcaacc
     9841 gacccccgcc gccgcagtgg ctgatatcgt cgccatcgcc gccggctgcg gtctttccaa
     9901 cagcgcctgg gcggccgacg aagaggattt cgagcgcctg gtcgatcagt ccctgtcggc
     9961 gaccgcgccg acgctgattg cggtgcgtat cgacgacaag ccgggcgtcg gcgcgacgcg
    10021 acgcgatccc gtgcagatca gggagcg
//
LOCUS       DASWGM010000825         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2047657,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000825 DASWGM010000000
VERSION     DASWGM010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047657"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VGA15_22765"
     CDS             <1..571
                     /locus_tag="VGA15_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9590552.1"
                     /translation="KVEPGHRVLIHAAAGGIGMLACQWARALGAHVIGTAGTRAKADL
                     ALANGCDHVILYNDEDFVARVKQISRNELCDVVYDGVGKTTFPGSLSCIKPRGLFVSF
                     GNASGPVPPFSIAELNNHGSLFATRPKLNDYIGKRSELLEGADTLFAAVINGKLHVPI
                     NHAYALKDVARAHVDLESRATTGAAILRP"
     gene            complement(760..1956)
                     /locus_tag="VGA15_22770"
     CDS             complement(760..1956)
                     /locus_tag="VGA15_22770"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEX9590553.1"
                     /translation="MTMATHPFDALMNITARPPTVFVRGEGSYLWDDTGKRYLDFIQG
                     WAVNCLGHSPPEVAEALAEQAKLLLTPSPAFYNGPSLKLAKALTDHSCFDQVFFTNSG
                     AEANEGAIKLARKYGALHKNGAYEIITFEGAFHGRTLATMSASGKKAFEPLFEPKVSG
                     FPKGKLNDLASVKRLINDNTVGVMLEPIQGEAGVWPATDQFLRELRTLTKAHGLLLIF
                     DEIQTGMGRTGKLFHYEHAGIEPDIMTLGKGIGGGVPLAALLATDAASCFEHGDQGGT
                     FNGNPLMCAAGLAVLDRVAAPDFLKSVVDSGLFLESELQRLSARHGLGEIRGRGLLQA
                     LDLKHPIGASIVAQAREDGVLLNSPQPDALRFMPALNVTREKIAAMIECLDAILTRMG
                     AARRVA"
     gene            complement(2189..>3286)
                     /gene="rimO"
                     /locus_tag="VGA15_22775"
     CDS             complement(2189..>3286)
                     /gene="rimO"
                     /locus_tag="VGA15_22775"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX9590554.1"
                     /translation="IVTGCMGAEPEQIEAAYPGVLSITGPQQYESVLDAVHRALPPVH
                     NPHLDLVPPQGIKLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPAGDVLREAEK
                     LVTAGVKELLVISQDTSAYGVDLKYAASPWKDRELRARFFDLAKELGEFGAWVRLQYV
                     YPYPHVDEVIGLMTQGKVLPYLDIPFQHASPEILKAMKRPAAQDKTLARIKKWREECP
                     ELTLRSTFIVGFPGETDSDFAYLLDWLEEAEIDRVGCFKYEPVAGAPSNALGNAVPDA
                     IKQERWNALMARQQKISARRLKRKVGTRQQVIIDEVGPTVSKGRSKADAPGIDGAVYV
                     SSRRPLRTGEIVTAKIERADEYDLHGSVAGF"
BASE COUNT          597 a         1030 c         1029 g          630 t
ORIGIN      
        1 caaagtcgag ccgggacatc gggttctgat ccacgcggcg gcaggcggca ttggaatgct
       61 cgcgtgccag tgggccaggg cgcttggcgc ccatgtcatc ggcaccgccg gcacccgggc
      121 gaaggccgac ctcgcgctcg ctaatggctg cgatcacgtc atcctctata acgacgagga
      181 ttttgtcgcg cgcgtcaaac agatcagccg caacgagctt tgcgacgtcg tctacgacgg
      241 cgtcggcaag acgacgtttc cgggttcgct gtcctgcatc aagccgcgcg ggttgttcgt
      301 gagttttggc aacgcctcgg gtccggtgcc gccattctcg attgccgaac tcaacaacca
      361 cggctcgctg tttgcgaccc ggccgaaact caacgactac atcggcaagc gctcggaact
      421 gctggaaggc gccgacacgt tgtttgctgc cgtcatcaac ggcaagctgc acgtgccgat
      481 caaccacgcc tacgcgctga aggatgtcgc cagggcgcat gtcgatctcg agagccgcgc
      541 gacgacgggg gctgcgatct tgcggccgta gattccatcg cctcctcgcg tcgtcccggc
      601 gaaggccggg acccataacc cctgtcctta aaagtgaaaa aggtcttcag ctccagtgcc
      661 gaaacgagag tccgcgccgt atgggtcccg gccttcgccg ggacgacatg cggggaggct
      721 tcgttcgccg ccgatcggcc cagaaagcgt cgacgcaacc tacgccacgc gccttgccgc
      781 gcccatcctg gtcaggatcg catccaggca ttcgatcatc gcggcgattt tttcccgtgt
      841 gacgttgagc gccggcatga accgcagcgc gtccggctgc ggcgagttga gcagcacgcc
      901 atcttcacgc gcttgcgcaa cgatcgacgc gccgatgggg tgtttcaagt cgagcgcctg
      961 cagcaaccct cggccgcgga tctcgccgag accgtgtcgc gcggaaagcc gctgcagttc
     1021 gctttccaga aacaacccgc tatcgacaac cgatttcaag aagtccgggg cggcgacgcg
     1081 gtcgagaacg gcgagcccgg ctgcgcacat cagcggattg ccgttgaagg tgccgccctg
     1141 atcgccatgt tcgaaacagg aggcggcgtc ggtcgcgagc agcgcggcga gggggacgcc
     1201 accgccgatg ccctttccga gtgtcatgat gtcaggctcg atgccagcat gctcgtagtg
     1261 gaaaagcttt ccggtgcggc ccatgccggt ctggatctcg tcgaagatca gcaatagacc
     1321 gtgcgccttg gtcaacgttc gcaactcgcg caggaactga tcggtcgcag gccagacgcc
     1381 ggcttcgccc tggatcggct ccagcatcac gccgacggtg ttgtcgttga tcagccgctt
     1441 gaccgacgcg agatcgttga gcttcccctt gggaaagcct gacaccttgg gttcgaacag
     1501 cggctcaaag gccttcttgc ccgaggccga catcgtcgcc agcgtccggc catgaaacgc
     1561 gccctcgaag gtgatgatct cgtacgcgcc gttcttgtgc agggcgccgt atttgcgcgc
     1621 gagtttgatt gcgccttcgt tggcttccgc gccggaattg gtgaagaaca cctgatcgaa
     1681 gcaactatga tcggtcagcg ccttggccag cttgaggctg gggccgttgt agaatgccgg
     1741 gctcggcgtc agcagcagtt ttgcctgttc ggcgagcgcc tcggcgacct cggggggcga
     1801 atggccgagg caattgacgg cccacccctg gatgaagtcg agatagcgct tgccggtgtc
     1861 gtcccacagg tatgagcctt cgccccgcac gaatacggtc ggcggtcgcg ccgtgatgtt
     1921 catcagcgcg tcaaacggat gtgtggccat ggtcatgtcg aactccctgt gggttaagat
     1981 gaaaaagccg gccgaaacgc gagaaggccg cactttgcgg gtgcagcctt ctcgaaaact
     2041 taagctgatc tatctgttca gcgttgtcgt cggacacggc gcgacccatc atcgtcgaac
     2101 gagcgacgac ggaaagctgc gcggagttgg gtccgagtga agatcatgat gccgccatac
     2161 agccgaacgg ccggtgctgt caagtgcgtc agaaccccgc gacgctgccg tgcagatcgt
     2221 attcgtcggc gcgctcgatt ttcgcggtga cgatttcgcc tgttcgcagc ggacggcggc
     2281 tggagacata gaccgcgcca tcgatcccag gcgcgtcggc cttggagcgg cctttcgata
     2341 cggtaggacc gacttcgtcg atgatgacct gctgccgcgt gccgaccttg cgcttcagcc
     2401 ggcgtgccga aatcttctgc tgccgcgcca tcagcgcgtt ccagcgctcc tgcttgatcg
     2461 cgtcaggcac ggcgttgccg agcgcattcg acggagcgcc tgcgaccggt tcatatttga
     2521 agcaaccgac gcggtcgatc tcggcctctt cgagccagtc gagcagatag gcgaagtctg
     2581 aatcggtttc gccgggaaag ccgacgatga aggtggaacg cagcgtcagc tccgggcatt
     2641 cctcgcgcca cttcttgatc cgcgccagcg tcttgtcctg cgccgccggg cgcttcatgg
     2701 ctttcaggat ttctgggctg gcgtgctgga acggaatatc gagatagggc aatactttgc
     2761 cctgcgtcat caggccgatc acttcatcga catgggggta ggggtagaca tattgcagcc
     2821 gcacccaggc gccgaattcg ccgagctctt tggccagatc aaaaaaccga gcgcgaagtt
     2881 cgcgatcctt ccacgggctc gccgcatact tcaaatcgac gccgtaagct gacgtgtcct
     2941 gcgaaatgac cagtaattcc ttcacaccgg ccgttaccag tttttcggcc tcccgcagca
     3001 cgtcgcccgc cggacgcgac accagatcgc cgcgcagttt cgggatgatg cagaagctgc
     3061 agcggttgtt gcagccctcg gaaatcttca aataagcgta atgccgtggc gtcagcttga
     3121 tgccctgcgg cggcaccagg tcgagatgcg gattatgcac cggcggcagc gcgcgatgca
     3181 cggcgtcgag cacgctttca tattgctgcg gtcccgtgat cgacagcacg ccgggatagg
     3241 ccgcctcgat ctgctcgggc tcggcgccca tgcagccggt gacgat
//
LOCUS       DASWGM010000826         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2472795,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000826 DASWGM010000000
VERSION     DASWGM010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472795"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VGA15_22780"
     CDS             <1..733
                     /locus_tag="VGA15_22780"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX9590555.1"
                     /translation="PLCVAIDPLDGSANLENNISVGTIFSIRPKGNDILSTFFEPGTA
                     QCAAGFVVYGPQTTLVLALNARVDIFILDRRTAEFRLIAQGVRVAPDTPEFAINASNR
                     RHWYGPVRAYIDECLAGTNGGGDADFNMRWIGSLVAESLRILVRGGVFLYPADARPGY
                     REGRLRLLYEAHPMALVMEWAGGAATTGRRRILELGAKTPHQRVPLIMGSLRGVRDVA
                     AIHEGVEPMFDNSDAPLFARRGLFL"
     gene            748..1623
                     /locus_tag="VGA15_22785"
     CDS             748..1623
                     /locus_tag="VGA15_22785"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEX9590556.1"
                     /translation="MSRSHPIISITGSSGAGTTSVKKTFENIFRREKVSAAYIEGDAF
                     HRYNRVEMRTKMAEEGERGNKHFSHFSPETNLFEELERLFRDYGESGTGTTRHYVHDD
                     DEAKLYGAEPGTFTEWAPLPEKSDLLFYEGLHGAVVTDKVNIAQYADLKIGVVPVINL
                     EWIQKLHRDRSDRGYTQEAVTDTILRRMPDYVNYICPQFAETDINFQRVPTVDTSNPF
                     IARWIPTADESMVVIRLRNPRGIDFPYLLSMIPNSFMSRANSIVIHGSKLDLAMQLIL
                     TPLILQLMERKRRTV"
     gene            1620..3659
                     /gene="tkt"
                     /locus_tag="VGA15_22790"
     CDS             1620..3659
                     /gene="tkt"
                     /locus_tag="VGA15_22790"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9590557.1"
                     /translation="MSIHALSSFAGRSDVSHQELANAIRFLAIDAVEKAKSGHPGMPM
                     GMADVATVLFSHFLKFDASDPVWPDRDRFVLSAGHGSMLLYALLYLTGYEGVTLDELK
                     AFRQWGSKTPGHPEYGHTPGVETTTGPLGQGIATAVGMALAERLLNAQFGNDFVDHHT
                     YVIAGDGCLMEGLSHEAISLAGHLRLGKLIVLFDDNQISIDGSTSLSCSDDQLARFKA
                     SGWSAERIDGHDPQAIAAAIERARSNDRPSLIACRTVIGFGAPNRQGTEKAHGAPLGA
                     EEVAKTRVALDWPYEPFQLPEHIVAQWRKIGARGQAARRSWMERTRRVNSVPRSSFHD
                     ALNRKLPCEYADAIDQLCTRFGAERPTIATRQASQLVIDAIAEALPNLLGGSADLTHS
                     NLTRAKTQRPVRADFFEGNYIHYGVREHAMAAAMNGIALHGGFIPYGGTFLTFADYSR
                     PAIRLAALMGIRVIHVMTHDSIGLGEDGPTHQPVEHLTSLRAIPNLLVFRPGDAVETA
                     EAWDCALRAEANPSVLCLSRQALPAFREAAGGANLVALGAYVVIEPAGGRDVTLIATG
                     SEVSIALEAAKLLAKDNVRAAVVSAPCFDLFRNQSSEYRAEVLGSAPRIGVEAAVEGD
                     WARWLGDSGEFVGMAGFGASAPAEVLYREFGITTEAVAKAAQRSIVRSRMAATYA"
     gene            3696..4781
                     /gene="fba"
                     /locus_tag="VGA15_22795"
     CDS             3696..4781
                     /gene="fba"
                     /locus_tag="VGA15_22795"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473339.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX9590558.1"
                     /translation="MARITLRQLLDHAAERGYGVPAFNINNMEQGLAIMEAAAAVDAP
                     VIMQASRGARSYANDIMLSRMIDALEEMYPQIPLCMHQDHGNEEATCATAIKYGFTSV
                     MMDGSLQADAKTAADYDYNVDITRRVVEMAHWIGASVEGELGVLGSLERGGGEQEDGH
                     GIEGKVSHDQLLTDPDQAVDFVRATKVDALAIAMGTSHGAYKFSRQPDGEILAMKVVE
                     EIHRRLPNTHLVMHGSSSVPQPLQDAFNQFGGEMPQTWGVPVEEIVRGIRHGVRKVNI
                     DTDCRLAMTAAFRKVAARSRSEFDPRKFFKPAMDAMRDLCRERFEQFGTAGHASKIKV
                     VPLAEMAKRYRAGSLDPRIGNIADAAE"
     gene            4835..>5205
                     /gene="rbcL"
                     /locus_tag="VGA15_22800"
     CDS             4835..>5205
                     /gene="rbcL"
                     /locus_tag="VGA15_22800"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046248.1"
                     /note="type III RuBisCO; involved in carbon fixation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HEX9590559.1"
                     /translation="MNELSMTVRRKDRYKSGVMEYKKMGYWEPDYTPKDTDIIALFRV
                     TPQDGVDPIEASAAVAGESSTATWTVVWTDRLTAAEKYRAKCYRVDPVPNSPGQYFAY
                     IAYDLDLFENGSIANLSASIIG"
BASE COUNT         1023 a         1591 c         1616 g          975 t
ORIGIN      
        1 gccactctgc gtcgccatcg atccgctcga cggatcggcc aaccttgaaa acaacatctc
       61 ggtcggcacc atcttttcga tccgccccaa gggcaacgac atcctctcga ccttctttga
      121 accgggcacg gcgcagtgcg cggcaggttt cgtcgtctac ggtccgcaga ccacgctcgt
      181 gctcgcgctc aacgcgcgcg tcgatatctt catcctcgac aggcgcacag cggaattccg
      241 gttgatcgcc cagggcgtcc gcgtcgcacc ggatacgccg gaattcgcca tcaacgcgtc
      301 caatcgccgg cactggtatg ggcccgttcg cgcctacatc gacgaatgcc tcgccggtac
      361 caacggaggc ggcgacgcgg atttcaatat gcggtggatc gggtcgctgg tcgcagaatc
      421 cctgcgcatc ctggtgcgcg gcggcgtgtt tctttatccg gccgacgccc gccccggata
      481 tcgcgaaggc aggcttcggc ttctgtatga agcgcatccc atggcgctcg tcatggagtg
      541 ggcgggaggt gctgcgacca cagggcgccg gcgaattctg gaattgggcg cgaaaacgcc
      601 gcatcagcgg gtgccgctga ttatgggatc gttgcgtggc gttcgcgacg tcgcagcgat
      661 tcacgaaggt gttgaaccga tgttcgacaa tagcgacgca ccgctgtttg cgcggcgcgg
      721 tctgtttctc tgagctggac aggtccgatg tcgcgcagcc atcccatcat atcgatcacg
      781 ggttcatcgg gcgccggcac gacgtctgtc aaaaaaacgt tcgaaaacat cttccgccgc
      841 gaaaaggtgt ccgcggccta tatcgaaggc gacgctttcc atcgttacaa ccgtgtcgaa
      901 atgcgcacga aaatggcgga ggaaggggag cgcggaaaca agcatttcag ccatttcagc
      961 cccgaaacca atctcttcga agagctcgag aggctgtttc gcgactacgg cgaatccggc
     1021 accggaacca cccgtcatta cgtccacgat gacgacgagg ccaagctgta tggcgcagaa
     1081 ccgggcactt ttaccgaatg ggccccgctg ccggaaaagt ccgatctgct gttttatgaa
     1141 ggtctgcatg gcgcggtcgt gaccgacaag gtcaatatcg cacaatacgc cgatctcaag
     1201 atcggcgttg tgcccgtcat caatctggag tggatccaga agctgcaccg cgaccggagc
     1261 gaccgcggct acacccagga ggcggtgacc gacaccatcc tgcggcggat gccggactac
     1321 gtgaattaca tctgtccaca gttcgccgag accgacatca atttccagcg cgtgccgacg
     1381 gtcgatacgt cgaacccgtt cattgcgcgc tggattccga cggcagacga atcgatggtg
     1441 gtgatccggc tcaggaaccc gcgcggcatc gattttccct atctgttgtc gatgattccg
     1501 aacagcttca tgtcgcgcgc caattcgatc gtgattcacg gatcgaaact cgatctcgcg
     1561 atgcagctca ttctcacccc gttgattctg caactgatgg aacgcaagag gcgcacagtg
     1621 tgagtattca tgctctttct tcattcgccg gtcgttcgga tgtctcgcat caagaattgg
     1681 caaatgcgat tcgctttctg gcgatcgatg cggtcgaaaa agcgaagtcg ggtcatccgg
     1741 gcatgccgat gggcatggcg gacgttgcca cggtgctgtt ctcgcatttc ctcaaattcg
     1801 acgcttccga tccggtgtgg ccggatcgcg accggtttgt gctttccgcc ggtcacggct
     1861 cgatgctgct gtatgcgctg ttgtatctga cgggctacga gggcgttaca ttagacgaac
     1921 tcaaggcatt caggcagtgg ggatccaaga cgccgggtca tcccgagtat ggccatacgc
     1981 cgggcgtcga gaccaccacc ggacccctgg ggcagggcat cgccacggcg gtcggcatgg
     2041 cgttggcgga gcgcttgttg aacgcgcagt tcggcaacga tttcgtcgat caccatacgt
     2101 atgtaattgc cggcgatggc tgcctgatgg aaggcctcag ccacgaagcg atttcgctgg
     2161 ccgggcatct ccggcttggc aagctgatcg tgctgttcga cgacaaccag atctcgatcg
     2221 acgggtcgac atcgctgtcc tgttcggatg accagcttgc gcgcttcaag gcctcgggct
     2281 ggtcggctga gcgtatcgac gggcacgatc cgcaagccat tgcagcggcg atcgaacgcg
     2341 ccaggagcaa tgaccgtcca tcgttgatcg cctgccgcac ggtcatcggc ttcggcgcac
     2401 cgaaccgcca gggcaccgaa aaagcccacg gcgcgccact cggtgccgag gaggtcgcca
     2461 agacacgcgt tgcgctggac tggccatacg aacctttcca gcttccagaa cacatcgtcg
     2521 cgcaatggcg caagatcggc gcaagggggc aggccgcccg ccggagctgg atggagcgga
     2581 caaggcgtgt caattcggtc ccgcgctcgt cgttccacga cgcgctgaac cggaaactac
     2641 cctgcgaata cgctgatgcg atcgatcagc tctgcacccg tttcggcgct gagcgtccga
     2701 ccatcgccac ccggcaagcg tcccagctgg tgatcgacgc gatcgcggaa gcgttgccga
     2761 acttgttggg gggctcggct gatctaaccc actccaacct gacgcgcgcc aagacacagc
     2821 gtccggtgcg ggccgatttc ttcgaaggca actatatcca ctatggcgtc cgcgagcatg
     2881 ccatggcggc agcaatgaat ggcatcgcgt tgcatggcgg cttcattccc tacggcggca
     2941 cgtttcttac cttcgccgat tacagccggc cggcgatccg gctggccgcc ctgatgggca
     3001 tcagggtcat ccacgtgatg acgcacgact ccatcggatt gggtgaagac ggtcccaccc
     3061 atcagccggt cgagcatctc acctcgctgc gggcgattcc taatctcctg gtgtttcggc
     3121 cgggcgacgc cgtcgagacc gccgaggcct gggactgcgc gctacgggcg gaagccaatc
     3181 cctcggtgct gtgcctgtcg cggcaggcgc tgccggcgtt tcgtgaagct gcgggcggcg
     3241 ccaatctggt tgcgctgggc gcctatgtcg tcatcgagcc cgccggtggc cgcgacgtca
     3301 cgctgatcgc aacgggctcg gaggtatcga tcgcgcttga ggctgcaaaa ctgcttgcga
     3361 aagacaacgt acgcgctgcg gtcgtgtccg cgccatgctt cgatctgttt cgtaatcaat
     3421 cgagtgagta tcgggccgaa gtccttggaa gcgccccgag gatcggcgtc gaggccgcgg
     3481 tcgaaggcga ttgggcgcgc tggctcggcg acagcggcga attcgtcggc atggccggct
     3541 tcggtgcatc cgctccggcg gaggtgctgt atcgcgaatt cggcatcacc acggaggctg
     3601 tcgccaaggc agcccagcgc agcatcgtgc gatcgaggat ggcggcaaca tacgcatgac
     3661 gcttgcaccg gacaaagaat gacacggaga aaacgatggc acgaatcacg ctgaggcaat
     3721 tgctggacca tgccgccgaa cgcggctatg gcgtgccggc attcaatatc aacaacatgg
     3781 agcagggcct tgcgatcatg gaggcggcag cggcggtcga tgcgcccgtc atcatgcagg
     3841 cctcgcgcgg cgcgcgctcc tacgccaacg acatcatgct gtcgaggatg atcgacgcgc
     3901 tggaggagat gtatccgcag attccgctct gcatgcatca ggaccacggc aacgaggaag
     3961 ccacctgcgc cacggcaatc aaatacggtt ttacttcggt catgatggat ggctcgctgc
     4021 aagctgacgc caagaccgcc gcagattatg actacaacgt cgatatcacc cgccgtgtgg
     4081 tcgagatggc gcactggatc ggtgcttcgg tggaaggcga gttgggggtg ctcggctcgc
     4141 tcgaacgtgg cggcggcgaa caggaagatg gccacggcat cgaaggcaag gtcagccacg
     4201 atcagttgct gacggatccg gatcaagcgg tcgattttgt ccgcgccacc aaggtcgacg
     4261 cgctggcgat cgcgatgggc acctcgcatg gcgcctacaa gttctcgcgc caaccggacg
     4321 gtgagattct tgcgatgaag gtggtcgagg aaatccaccg ccggctgccg aacacgcatc
     4381 tggtgatgca cggttcgtcg tcggtgccgc agcctttgca agacgccttc aaccagttcg
     4441 gcggcgagat gccgcagacc tggggcgtgc cggtcgagga gatcgttcgc ggcatccggc
     4501 atggcgtccg caaggtcaat atcgacaccg actgccggct ggcgatgacc gcggcgttcc
     4561 ggaaagtcgc ggcccggagc agaagcgagt tcgatccgcg caaattcttc aagccggcaa
     4621 tggatgcgat gcgcgacctc tgccgcgagc gttttgagca attcggtact gccggccatg
     4681 cgtcgaagat aaaggtcgtt ccgctggctg aaatggcaaa acgctatcga gccggatcgc
     4741 tcgacccgcg catcggaaac atcgccgacg ccgccgaatg acctgaagac cgtcaacccg
     4801 gatccgccag tctcgcgact taaggagaag agccatgaac gaattgtcga tgaccgtccg
     4861 ccgcaaagac cgctacaaat ccggcgtgat ggaatacaag aagatgggct attgggagcc
     4921 cgactacacg ccaaaggaca ccgacatcat cgcgctgttc cgggtgacgc cgcaggacgg
     4981 cgtcgatccg atcgaggcct cggcggcggt agccggagag tcgtcgaccg cgacctggac
     5041 ggtggtgtgg accgaccgcc tgaccgcggc cgagaaatat cgcgccaaat gctaccgcgt
     5101 cgatccggtg ccgaattccc ccggacaata ttttgcttac atcgcctacg atctcgacct
     5161 gttcgagaac ggatcgatcg ccaacctgtc ggcttcaatc atcgg
//
LOCUS       DASWGM010000827         5849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2705525,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000827 DASWGM010000000
VERSION     DASWGM010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2705525"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VGA15_22805"
     CDS             <1..476
                     /locus_tag="VGA15_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643801.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX9590560.1"
                     /translation="AEDILAMKPDGVFLSNGPGDPAETGKYAVPVIQKVINSGTPTFG
                     ICLGHQMLGLAVGAKTRKMHQGHHGANHPVRDETTGKVEITSMNHGFAVDQATLPKGA
                     TQTHVSLFDGSNCGISLDGKPVFSVQYHPEASPGPRDSHYLFKRFADLMRAKKNG"
     gene            692..1186
                     /locus_tag="VGA15_22810"
     CDS             692..1186
                     /locus_tag="VGA15_22810"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9590561.1"
                     /translation="MDDATAPKIEYGVTPTEVMAAMAGIDFVRAIFDGKLPVPPIMQS
                     IEPFDSTAEPGVVVMYSTPGFRHYNPIGSVHGGYAATLLDSAMGLAVHSTLPAGSGYT
                     TLEFKVSFIRGMTKDSGPVRTEGCILNVGRRAATAEARITDANGRLLAHATTTCLVFE
                     IPKA"
     gene            1252..1776
                     /locus_tag="VGA15_22815"
     CDS             1252..1776
                     /locus_tag="VGA15_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590562.1"
                     /translation="MPVVSADIEPLAFTFEDDGLVPNNPMPFLVYKQAISVDNEHPEK
                     TIEGLFGANGWGEMWRNGVYDYLHYHATVHEVLGVARGHARVRFGGDAGKEIEIAAGD
                     VAILPAGTGHQCLSASEDFCVIGAYPPGAKMHVTLPTLENHAKALKTIPAVKLPRTDP
                     VMGEDGSLLRLWKS"
     gene            complement(1948..3057)
                     /locus_tag="VGA15_22820"
     CDS             complement(1948..3057)
                     /locus_tag="VGA15_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX9590563.1"
                     /translation="MIIILCLYIVALWLVFSKFKLVRWGWLSGTVSVLIGVFILATFL
                     ALFNYLTPSGRVTVTGRVVEVTPNVTGQIVAIPVKPNVPVKANDVLFQIDPAPFQYKV
                     SQLQASLAAAKQQTEILKSNYDQASANVVGLTAQVAFNAKRLGDIQKLAAEDANTQFQ
                     AQDRQNQYETALAQLNVAKAAQQSAKLALDSEIGGVNTTVAQIQAQLDNASWELSQTA
                     IRAPADGYVTLVTLSVGDRALQARSAMSFIVENEITLVGMFSQNGFQTIKDGASVDIV
                     FDNAPGRIYHAKITAIPKGVGQGQVAVSGTLARTNVLGGATVFPAEISIPDGMSRESL
                     RLGMSGSATAFADNAGVIGLLASILVWISSYTAYL"
     gene            3352..4809
                     /locus_tag="VGA15_22825"
     CDS             3352..4809
                     /locus_tag="VGA15_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX9590564.1"
                     /translation="MLRGVLTLTLSLFLGAAAAPAALAQSAAPAITEQEAHAIAVDAY
                     VYFYSIMSMDVSRKQFTNGTTDFKGPMNTFVNVPEYPPANFKGVVRSNFDTLYSVSWL
                     DMTKEPVVISAPDTDGRYYLLPMLDMWTDVFASPGWRTTGTKAGTFLVTPAGWRPDLR
                     DRFADEFKLPKDTQRIDAPTPYVWVIGRTKTDGPPDYDAVHKIQAGYKVTLLSEFGKT
                     PKPIEVKIDPSVDMKTPPKVQVDTMTAGVYFAYAAELLKLHPPHVTDEPIIAQMRKIG
                     IEPGKSFDIGKLDPVLQRALETAPQDGQKLMAWKVPTLARVANGWSMNTDTMGVYGNY
                     YLKRAIVSQVGLGANLPEDAIYPLNLADESGKPLDGAHKYTITFEKGAAPPVNAFWSI
                     TLYDQEGFQVGNALNRFAVSSWMPFKNNADGSLDLYFQNESPGKDKEANWLPAPKGSF
                     NLTMRLYAPKSEALTGKWNPPPIVKTPETVGLSAQ"
     gene            4955..>5849
                     /locus_tag="VGA15_22830"
     CDS             4955..>5849
                     /locus_tag="VGA15_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX9590565.1"
                     /translation="MLTKRDLLRSAALAAITASTAKAVPASAQAKAEWPGIIEAKAIA
                     EEGFIYGLPIVMNYAVMYEYSVDKNSGQYKAPFNQIKNEARVYTYKDTAVITPNSDTP
                     YSLLWLDLRAEPVVLSVPAVEKTRYYSVMLCDGNTFNYGYIGSRATGSEAGDYMVVGP
                     NWQGATPPGIKKVFQSTTQFSAAAYRTQLFNAADMPNVEKVQAGYKVQALSAYLKQPA
                     TTAAAAIDFPKIDKELVKKNFFEYLDFALQFAPPGPEERVIREKLARIGVGPGKTFDF
                     KDLPLEHKLEIGLGMKDGGEKV"
BASE COUNT         1257 a         1759 c         1695 g         1138 t
ORIGIN      
        1 cggccgaaga catcctggcg atgaagcccg acggcgtgtt tttgtcgaac ggacccggcg
       61 atcccgccga gaccggcaaa tacgcggtgc cggtgatcca gaaggtgatc aattcgggca
      121 cgccgacctt cggcatttgc ctcggccatc agatgctggg cttggcggtc ggcgccaaga
      181 ccagaaagat gcatcagggc catcacggcg ccaatcatcc ggtcagggac gagaccaccg
      241 gcaaggtgga aatcacctcg atgaaccacg gctttgccgt ggatcaggcg acgctgccga
      301 agggcgcgac ccagacccac gtgtcgttgt tcgacggctc caattgcggc atctcgctcg
      361 acggcaagcc ggtgttctcg gtgcagtatc acccggaagc ttcccccgga ccgcgcgact
      421 cgcattatct gttcaaacgg ttcgccgatc tgatgcgggc gaagaagaac gggtagctat
      481 tgatccggcg tgccccggat gctgcgcagc gcgccgcgct ttgcggcgtg atgcgctgct
      541 gatccggggc ccatcctgca tcgcgcccgt gggtcccggc tctgcggagc agcgttacac
      601 gctgcgccgc gtccgggaca cgagagttgc gagtgcaggc gccgtttaat atgatgatca
      661 tcatcaaaaa ccgccgcagg aaataatcct catggacgac gcaaccgccc ccaaaatcga
      721 atacggcgtg acgccgacgg aagtcatggc cgcgatggcg ggtatcgatt tcgtccgcgc
      781 catcttcgac ggcaaattgc cggtgccgcc gatcatgcag agcatcgaac ccttcgattc
      841 caccgccgag cccggcgtgg tcgtgatgta cagcacgccc gggttccggc attacaatcc
      901 gatcggctcg gtgcatggcg gctatgccgc gaccttgctg gattcggcga tggggctggc
      961 cgttcacagt acgctgccgg cgggatccgg ctacaccacg ctcgaattca aggtctcctt
     1021 catcaggggc atgaccaagg atagcggtcc ggttcgcacc gaaggctgca tcctgaacgt
     1081 cggccgccgc gcggcgaccg ccgaggcacg tattaccgat gccaacggcc gcctgctcgc
     1141 ccacgccacc acgacctgtc tggttttcga gattcccaaa gcgtgatcga cgggaacacg
     1201 agccgctgac aacagttgaa tccgcgctta tttcaactgc ggagaattgc catgccagtg
     1261 gtcagcgccg acattgaacc gctcgccttc acgttcgagg acgacggcct cgtcccgaac
     1321 aatccgatgc cgtttctggt ctacaagcag gcgatatcag tcgataacga acaccccgaa
     1381 aaaaccatcg aaggactgtt cggcgccaat ggctggggcg agatgtggcg caacggcgtc
     1441 tacgattatc tgcactacca cgcgacggtc catgaagttc tcggcgtcgc gcgcggccat
     1501 gcgcgggtcc ggttcggcgg cgacgccggc aaggaaatcg agatcgcggc gggcgatgtc
     1561 gcgatcctgc cggccggaac cggacatcaa tgcttgtcgg cgagtgagga tttctgcgtg
     1621 atcggggctt atccgcccgg ggccaaaatg catgtcacgc tgccgacgct ggaaaatcac
     1681 gccaaggcgt tgaagacgat tccggcggtc aagctgccca ggaccgaccc ggtgatggga
     1741 gaagacggat cgctgctgag attgtggaag tcgtagggtg ggctccgcga gcccacccta
     1801 ctacccaaca ccttcccaac accttccatc tttacctgac atcttcgaat ggtgggcacg
     1861 ccgcagatgc gcctttgccc accctacgat tcattcggca gattgggctg ccaagagccg
     1921 aattcttaat tcccagccga cgaacggcta aaggtacgcc gtgtaggaac tgatccacac
     1981 caggatggac gcgagaaggc cgataacgcc tgcgttgtcg gcaaatgcgg tagcgctgcc
     2041 ggacattccc agacgaagtg attcacggct catcccgtca ggtatcgata tctccgccgg
     2101 aaagactgtc gcgccaccga gcacgttggt gcgcgcgagc gttcccgaca cggccacctg
     2161 tccttgaccc acgcccttgg gaatcgccgt aatttttgcg tgatagatgc gtcccggggc
     2221 gttatcgaaa acgatatcga cggaagcgcc gtccttgatg gtctgaaaac cattctgcga
     2281 aaacattccg acgagcgtga tctcgttctc gacaatgaag gacatcgccg agcgcgcctg
     2341 cagcgcccgg tcgccgaccg acaatgtcac gagcgtcaca tagccatcgg ccggtgcgcg
     2401 aatggccgtc tgcgacaatt cccagctggc gttatcaagc tgcgcctgaa tctgcgcgac
     2461 tgtggtgttg acgccgccga tctcggaatc cagcgcgagt ttggcgctct gctgcgccgc
     2521 cttggctacg tttagctgcg ccagcgccgt ctcgtattga ttctggcggt cctgcgcctg
     2581 gaattgtgtg ttggcatctt ccgccgcaag cttctgaatg tccccgaggc gtttcgcgtt
     2641 gaacgcgacc tgcgcggtca gaccgacgac attcgcactg gcctgatcgt aattcgattt
     2701 caagatctcg gtctgttgct ttgcagcagc gagcgaggct tgcaactgcg agaccttgta
     2761 ttgaaaaggc gccgggtcga tttgaaacag cacgtcgttt gccttcaccg gcacgttcgg
     2821 cttgaccgga attgcgacga tttgccccgt cacattcggt gtcacttcca ccacccgacc
     2881 ggtcaccgtg acgcgccccg acggcgtgag ataattaaaa agcgcgagaa atgttgcgag
     2941 aataaaaacg ccgataagca ccgagaccgt cccggacaac cagccccatc gcaccagttt
     3001 gaattttgaa aacacgagcc agagcgcgac gatgtaaagg cataaaataa tgatcatggc
     3061 tggtcctcat tatccactcg tgctaaggta ctacccctga tagagggctg cgtattacag
     3121 gtagaggcag ccgaagaggc ccttcgacag tcagctttaa tagcatctat ttttccgacc
     3181 ttgttcgatt ctgcaaacgc cgtacggcgt tttatgcagc gggcggtgca cgttctcttg
     3241 caagcaatac gcaacctctc gcgtcagagc gagaatgctt ccaaatcgtt cagtcgattt
     3301 tttgcagaag gaatggatct caagcgtatc atcagaagag gagtgttcga catgttgcga
     3361 ggagttctaa cactgacatt gtcactcttc cttggggcgg cggctgcgcc cgcggcgctg
     3421 gcccaaagcg cggcgccggc aatcaccgaa caggaggcgc acgctatagc ggtggacgct
     3481 tacgtttact tctactccat catgtcgatg gacgtgtccc gaaaacaatt caccaacggc
     3541 accaccgatt tcaaaggtcc gatgaacacg ttcgtcaacg tgcccgaata cccgccggca
     3601 aatttcaagg gcgtcgtacg ctccaacttc gatacgctct attccgtttc ctggctggac
     3661 atgaccaaag agcccgtcgt catcagcgct ccggatactg atggacgcta ctacctgctg
     3721 ccgatgcttg acatgtggac cgatgttttt gcgtcgccag gctggcgaac gacgggcacc
     3781 aaggccggga cttttctggt gacgcccgcc ggctggaggc cggatctgcg cgacaggttc
     3841 gccgatgagt tcaagcttcc aaaggatacc cagcggatcg atgcgccgac gccttatgtc
     3901 tgggttatcg gccgtaccaa gaccgacggc ccgccggact atgatgcggt tcataaaatc
     3961 caggccggct acaaggtcac cctgctttcc gaatttggaa agacgcccaa gccgatcgaa
     4021 gtcaaaatcg accccagcgt cgatatgaag acgccgccaa aggtccaggt cgataccatg
     4081 acggccggtg tatatttcgc ctatgcggca gagttgctca agcttcaccc gcctcatgtc
     4141 accgatgagc cgatcatcgc gcagatgagg aaaatcggta tcgagccggg taagagcttt
     4201 gacatcggca agctcgatcc agtcctgcaa agggcgctgg aaaccgcgcc gcaggacggg
     4261 cagaagctga tggcttggaa agtgccgacg ctggcgcggg tcgccaatgg ctggtcgatg
     4321 aacaccgata cgatgggtgt ttacggcaac tactatttga agcgggctat cgtctcgcag
     4381 gtgggacttg gtgcgaattt gcccgaggac gccatttatc ccctcaatct cgccgacgaa
     4441 tccggcaagc cgctggatgg tgcgcacaag tacaccatca cgtttgagaa aggcgctgcc
     4501 ccgccggtca atgctttctg gtcgatcacg ctttacgatc aggaaggatt ccaggtcggc
     4561 aacgccctga accgcttcgc cgtcagcagt tggatgccgt tcaagaacaa cgcggacggt
     4621 tcgctcgatc tttacttcca gaacgagagt ccgggcaagg acaaggaagc caactggctt
     4681 cccgcgccaa agggatcctt caatctgacc atgcgcctct acgctccgaa gtccgaggcg
     4741 ctgaccggaa aatggaatcc cccgcccatc gtgaagacgc cggaaacggt gggcctgtcg
     4801 gctcagtaaa aattaaccgg agcgccggcg cttcggcgcc ggcgccgtct cgatcattcg
     4861 ctcggtagag cgatctgtga agagaaggcc gctttagccg ccgcactcag atcagaaaac
     4921 cgaccccact ctagctgtat gaggagacgt ccaaatgctg acgaaacgcg atcttcttcg
     4981 ttccgctgcc ttggctgcga tcaccgcctc gacggcgaag gctgtcccgg cgagcgcgca
     5041 ggccaaagcc gaatggcccg gcatcatcga ggccaaggcc atcgccgagg aaggattcat
     5101 ctatgggctg cccatcgtga tgaattacgc tgtcatgtac gagtattccg tcgacaagaa
     5161 ctcggggcaa tacaaggcgc cattcaacca gatcaaaaac gaggcgcgtg tctatacgta
     5221 caaagatacg gccgtcataa ctcccaacag cgacacgccg tattcgctgc tatggctcga
     5281 tctgcgcgcc gaaccggttg ttctatcagt gccggctgtg gagaagacgc gctattactc
     5341 cgtgatgctg tgcgacggca acacgttcaa ttacggttac atcggcagcc gcgcgaccgg
     5401 cagcgaggct ggtgactaca tggtcgttgg gcctaactgg cagggtgcca caccgcccgg
     5461 catcaagaag gtattccagt ccacgacgca gttttctgcc gctgcctacc gcacgcagct
     5521 tttcaatgcg gcagacatgc cgaatgttga gaaggtacag gccggctaca aggtgcaggc
     5581 gctttccgcc tatctgaaac agccggcgac gactgccgcg gcggccatcg actttccaaa
     5641 aatcgacaag gagctcgtga agaaaaactt cttcgagtat ctcgacttcg cactgcaatt
     5701 cgcaccgccg gggccggaag aacgcgtcat tcgcgagaag cttgcccgca tcggcgtcgg
     5761 cccgggcaag accttcgact tcaaggatct tccgctggag cacaagcttg aaatcgggct
     5821 gggcatgaag gacggcggcg agaaggttg
//
LOCUS       DASWGM010000828         4980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2614310,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000828 DASWGM010000000
VERSION     DASWGM010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2614310"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VGA15_22835"
     CDS             <1..1341
                     /locus_tag="VGA15_22835"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX9590566.1"
                     /translation="VRPKQFLPLFGPRSTFQDTLRRVSDAALFERPIVITNMAYRFMV
                     LEQLAEIGLEADVLLEPMRRDSGPAIAAGAVFAQTRDNDAVVLALAADHVVRDTDAFV
                     AACRQGLVAADAGRIVTFGVQPERAATEYGYISPGEVISGAVRAVVEFVEKPDPVTAA
                     GYIKSGYLWNSGNFMFRASVLLDEYRNVDADSVQVVSDAVTKAGRDLGFVTLDPDSFA
                     KARAISIDYAVMEKTSHAAVVPVACGWSDVGSWLAVWELSDKDAQGNAAQGAAVFEDS
                     RNCNVSSDKALVALEGVDDLVVVATQDAILVSRQKDANGLKRLVAKLKTVAPHVTEDH
                     LKVHRPWGSYQSIDTGDRHQVKRIVVKPGNRLSLQKHHHRSEHWIVVRGTARVTVNEL
                     VKTVHENESIHIPIGAVHRLENPGKILLELIEVQTGSYLGEDDIIRIEDDYRRT"
     gene            1531..2547
                     /locus_tag="VGA15_22840"
     CDS             1531..2547
                     /locus_tag="VGA15_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9590567.1"
                     /translation="MISKGPASGGKAEAKRALRVGVIGAGIMGSNHARVLAGLPDTTL
                     VGVVDPLPAHRTRATELTGCRTFATLDELISEGIDAVTVAAPTHLHHEITLGCIAREI
                     HVLVEKPIASSVQEGQEIVDAARRAGVTLMVGHVERFNPAVAAIKAAISGEDILSIAI
                     TRVGPFPPRMSNVGVVIDLAVHDIDLIRWFTESDIVEVQPQLSSAVAEREDIALLQFR
                     TASGVLAHINTNWLTPFKARNVTVATRGKYVMGDLLTRQVTECFGFKPDGSYSMRHLP
                     VGHDEPLRAELIAFLGAVRSGGVPAVTGDEGVASLEIAIRCLETPARPAATAALKGPR
                     RVVG"
     gene            2588..3739
                     /locus_tag="VGA15_22845"
     CDS             2588..3739
                     /locus_tag="VGA15_22845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX9590568.1"
                     /translation="MNQHLRPEPVPFIDLATQRRRLGKSIDEAVSRVLTHCQFINGPE
                     VTQLEAALAAFSGAKHVVSCASGTDALLMVLMAKGVGRGDAVFCPSFTFCATGEAVAL
                     TGATPVFVDVDEATFNMDANSLKRGIAAAIKSGLKPRAVIPVDLFGQSADHDAIGAIA
                     EADGLFVLDDAAQAFGASYKGRRLGTFGLVTATSFFPAKPLGCFGDGGAIFTDDAELA
                     ETLRSIRVHGQGSDKYDNVRLGLTGRLDTLQAAILIEKLKIFEDEIAVRNVVAERYAR
                     GLGNIVSVPRPASGCTSVWAQYTIRLPKGSDRDAFAAALKAQGIPTAIYYPKSMHQQT
                     AYRDFPVADGGVPISEALSDDVISLPMHAYLDEPTQERIIRAVRGALSS"
     gene            3783..>4980
                     /gene="murJ"
                     /locus_tag="VGA15_22850"
     CDS             3783..>4980
                     /gene="murJ"
                     /locus_tag="VGA15_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX9590569.1"
                     /translation="MLGRIFTVGGYTLLSRLTGFARDIMLAAILGAGPVADAFFVAFR
                     LPNHFRAIFAEGAFNAAFVPAYAHVHGEHGETSARLFASRIFTLLFFSQLVLLVVAWL
                     FMPQAMRILAPGFTDDAEQRRLAIELTRITFPYLLLITLVTLYGGMLNVMHRFASAAA
                     ASILLNISMMATLALAAFFPGAGHAAAWGVLISGFLQYFLLAGDLAAHGGLPRFATLK
                     LDDDVRAFFRALGPATLGSMGTQVALFADTIIATFLPAGALSALYYADRLNQLPIGVI
                     GIAIGTVLLPEMSRRLTAGDHAGAMASQRRAFDFTLLFSVPFVAAFLTVPEIITRAMF
                     ARGAFSKADAAASGATLAAYAIGLIPFVLIRSAVATFYARKDTATPVKAALTGVAVNV
                     ALKIALV"
BASE COUNT          824 a         1562 c         1600 g          994 t
ORIGIN      
        1 gtgcgcccga agcagttcct gcctttgttc gggccgcgtt cgacgtttca ggacacgctg
       61 cggcgcgtct ctgacgccgc gctgttcgaa cgtccgatcg tgatcaccaa catggcgtat
      121 cgcttcatgg tgctcgagca attggccgag atagggcttg aggccgatgt gctgctggag
      181 ccgatgcggc gggattccgg gccggcgatc gccgcgggtg cggtctttgc ccagacgcgt
      241 gacaacgatg ccgtggtgct tgcgctcgcc gccgatcacg tcgttcgcga taccgacgct
      301 tttgttgctg cctgccgtca gggcctggtc gccgccgatg cgggacgcat cgtgaccttc
      361 ggcgtgcagc ctgagcgggc ggctaccgag tacggctata tcagccccgg cgaggtcatt
      421 tccggcgcgg tccgcgcggt cgttgaattt gttgaaaaac ccgatccagt gacggccgcc
      481 ggttacatca aatccggtta cctctggaac agcggcaact tcatgtttcg cgcatctgtt
      541 ctgctcgacg aatatcgcaa tgtcgatgct gacagcgtgc aagtggtgag cgatgccgtg
      601 accaaggctg gacgcgatct tggtttcgtg acgcttgatc cggattcgtt tgcaaaggcc
      661 agggcgatct ccattgacta cgcggtgatg gaaaagacct cgcacgccgc ggtggtgccg
      721 gtggcgtgcg gctggtccga tgtcggctcc tggcttgcgg tgtgggaact gtcggacaag
      781 gatgcccagg gcaacgccgc gcagggcgcc gcggtgttcg aggattcacg caactgcaac
      841 gtttcgagcg acaaggcgct ggtcgcgctg gaaggggtcg acgatctcgt ggtggtggcg
      901 acgcaggacg ccatactggt gtcgcgtcag aaggacgcca acggattgaa gcggctggtc
      961 gccaaactga agacggtagc acctcatgtc accgaggatc atctcaaggt gcaccgcccg
     1021 tggggaagtt atcaatcgat cgacaccgga gatcgtcacc aggtcaagcg catcgtcgtg
     1081 aaaccgggga accggctctc actgcaaaag catcatcacc gctccgagca ctggatcgtc
     1141 gtgcgcggca cggcgcgcgt aacggtcaat gaactcgtca agaccgtgca cgagaacgag
     1201 tcgatccata tcccgatcgg cgcggtccac cggctggaaa atcccggcaa gattctgctg
     1261 gaactgatcg aggttcagac cggcagttat ctcggcgagg acgacatcat ccgtatcgag
     1321 gatgattatc ggcgcacctg agcaattcat cgtttcgcct cgaatcaggc tccttttgga
     1381 tcatgttcgc tgcctgggcc ggcagcggga cggtgtaatt ttctgactca aaacgtgtcc
     1441 gggatagcct ctcggcattc gccacgattg tgacgaacgt gctaggagag ccacgggatg
     1501 ttcgtgtcga aacatgatcg gggctgattt atgatttcca aggggcccgc atcgggcggg
     1561 aaggccgagg caaaacgcgc gcttcgcgtc ggcgtcatcg gcgccggcat catgggcagc
     1621 aaccatgccc gcgtgctggc cgggttgccg gatacgacgc tggtcggcgt ggtcgacccg
     1681 ctgccggcgc atcggacgcg cgccaccgaa ctgaccggct gccgcacctt cgccactctg
     1741 gacgagctga tctccgaagg tatcgatgcc gtgacggtcg cagcgccaac gcacctccat
     1801 cacgagatta cactgggctg catcgcacgc gagattcacg tcctggtcga aaagccgatc
     1861 gcgtcctcgg tgcaggaggg gcaggagatc gtcgacgctg cacgccgcgc cggggtgacg
     1921 ctgatggtgg ggcatgtcga acgtttcaac ccggccgtcg ccgccatcaa agcagcgata
     1981 tcgggcgagg acattctttc gatcgcgatc acccgcgtcg gaccgtttcc gccgcgaatg
     2041 tcgaacgtcg gtgtcgtgat cgacctcgcc gtgcacgaca tcgatctgat ccgctggttc
     2101 acggagtcgg acattgtgga ggtgcagccg cagctttcca gcgccgtcgc cgagcgcgag
     2161 gatattgcac tattgcagtt ccgcaccgcc tccggcgtgc tcgcgcatat caacaccaac
     2221 tggctgacgc cgttcaaggc gcgtaacgtc accgtcgcca cccgcggtaa atatgtgatg
     2281 ggcgatctcc tcacccgtca ggtgaccgag tgcttcggct tcaagccgga cggcagctac
     2341 tcgatgcgcc atctgccggt gggccatgat gagccgctgc gggccgaatt gatcgcgttc
     2401 ctcggcgcgg tgcgctccgg cggcgtgccc gcggtgaccg gcgatgaagg cgtcgccagc
     2461 cttgaaatcg cgatccgttg cctggaaact ccggcaagac ctgccgcaac cgcagccctc
     2521 aaggggccgc gccgcgttgt cggctaatcg cgcgttaatg tcccattcca aatccttgca
     2581 aggctccatg aaccagcatc tgcgtccaga acccgtaccc tttatcgacc ttgccacgca
     2641 gcgccgccgg ctcggcaaat ccatcgatga ggcggtttcc cgggtgctca cccattgcca
     2701 gttcatcaat ggtccggagg tgacgcagct cgaggccgcg ctggcggcct tcagcggcgc
     2761 aaagcatgtc gtgagctgtg ccagtggcac cgacgcgttg ctgatggtgc tgatggccaa
     2821 gggcgtcggc cgtggcgacg cggtgttctg tccgtcgttc acattctgcg cgaccggcga
     2881 ggcggtggcg ctgacgggtg ctacgcctgt gttcgtcgat gtcgatgaag cgaccttcaa
     2941 catggatgca aattcgctga agcgcggcat cgcggccgcg ataaagtccg gcctcaagcc
     3001 cagggccgtg attccagtcg atctgttcgg gcaaagcgcc gaccatgacg cgatcggcgc
     3061 gatcgcggaa gctgacggcc tgttcgtgct cgatgacgca gcccaggcgt tcggcgcgag
     3121 ctacaagggc cgccgtctcg gcaccttcgg tctcgtcacc gcgaccagct ttttcccggc
     3181 aaaaccgctc ggctgctttg gcgatggcgg ggcgattttc accgacgatg ccgaactcgc
     3241 cgagacgctg cgcagcatcc gcgtgcacgg ccagggctcc gacaagtacg acaatgttcg
     3301 tctcgggctc accggaaggc tcgacaccct gcaggcggcg atcctgatcg agaagctgaa
     3361 aatcttcgag gacgagatcg ccgtgcgcaa cgtcgtcgcg gagcgctatg cgcggggtct
     3421 cggcaatatc gtgtcggttc cgcgtccagc cagcggctgc acctcggtgt gggcgcaata
     3481 caccatccgg ctgccgaagg ggagtgatcg cgacgctttt gccgcggcct tgaaggcgca
     3541 gggcattccg accgcgatct actatccgaa atcgatgcat cagcagaccg cctaccggga
     3601 tttcccggtc gccgacggcg gcgtgccgat cagcgaggcg ctgtcggacg acgtcatcag
     3661 cctgccgatg catgcctatc tcgatgagcc gacgcaggag cggatcatca gggctgtgcg
     3721 tggcgcgctt tcatcctgat ttcgccgttt tgaagcgccg ccgcatgctg tagaagcggc
     3781 gcatgctcgg tcgtattttc accgtcggcg gctacacgct cctttcgcgg ctgaccggct
     3841 ttgcgcgcga catcatgctc gcggcgatcc tcggcgccgg gccggtggcg gacgcatttt
     3901 tcgtggcttt tcgcctgccc aatcattttc gggcgatttt cgcggaaggc gcattcaatg
     3961 cggctttcgt gccggcctac gcccatgtcc acggcgagca cggtgagacg tcggcgcgat
     4021 tgttcgccag ccgtatcttc accctgctgt ttttctcgca actggttctg cttgttgtcg
     4081 cgtggctgtt catgccgcag gccatgcgca ttctcgcgcc cggcttcacc gacgacgccg
     4141 agcagcggcg gcttgcgatc gaactgacgc ggatcacgtt tccctatctg ctgctgatca
     4201 cgctggtgac gctctatggc ggcatgctca acgtgatgca tcggttcgcc agcgcggcgg
     4261 cggcgtcgat tctgctcaac atctcgatga tggcgacgct ggctctggcc gcatttttcc
     4321 caggagcagg ccacgctgcg gcatggggcg tcttgatttc cggctttctg caatacttcc
     4381 tgctggccgg cgatcttgcg gctcatggcg ggctgccgcg gttcgcaacc ctcaagctcg
     4441 acgacgacgt ccgcgcattc tttcgtgcgc tcggacccgc gacgctcggc tcgatgggga
     4501 cgcaggtcgc gttgttcgcc gacaccatca tcgcgacctt tctgccggcg ggcgcgctct
     4561 ccgcgctgta ttatgccgac cggctcaacc aactgccgat cggcgtgatc ggcatcgcca
     4621 tcggcacggt attgctgccg gaaatgtcgc ggcggctgac cgcgggcgat cacgccggcg
     4681 cgatggcgtc gcagcggcga gctttcgatt tcacgctgct gttttcggtg ccgtttgtcg
     4741 ccgcgttcct gacggtgccc gaaatcatca cgcgggcgat gttcgcccgc ggcgcgttct
     4801 cgaaggccga tgccgcggcc tcgggcgcca cgcttgccgc ctatgcgatc gggttgattc
     4861 cgttcgtgct gatccgcagc gcggtcgcaa ccttctacgc ccgcaaggac accgcgacgc
     4921 cggtgaaggc ggcgctgacc ggcgtcgcgg tcaatgtcgc gctgaagatt gcgctggtgg
//
LOCUS       DASWGM010000829         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2180848,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000829 DASWGM010000000
VERSION     DASWGM010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2180848"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3529)
                     /locus_tag="VGA15_22855"
     CDS             complement(<1..>3529)
                     /locus_tag="VGA15_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX9590570.1"
                     /translation="DIVRLRSIDISIVADTPEGSGVCGQAFRDQKVSISNDYLNDARS
                     RAWRQGACDAHIGAAAALPLTCNGRGVGVLLVTRREAGSLNDQIVSLFVRMSANISCA
                     LDNFDHEAARKSGERAMRRLNRMFGAISATNEAILRAKTEQELYQLVCDAAVNSGESI
                     ATVAFLAEPGSIWLKPVAGTGEIVELITRARFSIDPDDVHGTGVCGNAFRTQKPCINN
                     DILNSVQGRPWQQAGRETGVVACVALPLIKAGNSVGVLLFFVSKSWAADQEIIALLAR
                     MAENVSFALDNFERAAEKAKADEQKERLARMFASLSATNEAIMRAKSRAELFEMVCQA
                     AVHGAKFGSATVALAESDSLYLRVVASTGPGAGEMRLSEFAITESLPQGRGLTGSAFR
                     AQQPCVSNDFLADERTRPWHDHAIRSGVRSCAALPLLNGDRAAGVLIFNSPELGTFTP
                     ELVELLQRLAENVSFALGNFDRADEKAKAEKQKDRLTGMFEALSATNEAIMRAKTRAQ
                     LFELVCEAAVFGGTFSSATIALADPAGEFLRIAASKGINYDRMRSRRFAISAADPEGR
                     GLTGTSFRTREPCIMNDFLADERTTHWHALAREDGTRSGASFPLLKNGDRAVGVLLFL
                     SCDEAAFTDDLVELLGRLAENVSFALDNFDRVDEKQKTEEQKERLTRMFAALSATNEA
                     IMRAKSRVQLFEMVCEAAANGGKFTSTTISIANRDSDFLDIAAVAGPTAETTRNVKVS
                     ISAARPEGRGLSGAAFRSRQPCISNDYLADQRASAFHAIIRGDGAKSGAAFPLLIRGQ
                     AVGVMLFLSAEKDTFTPEFVELLQRLADNVSFALENFDRADEKTKADARIEYLASHDS
                     LTSLPNREMFNGLLHHAIEAACRHRRRFAVLFIDLDRFKVINDSLGHDAGDILLVEIA
                     NRLRQTLRSSDVVARLGGDEFVVILEETAERHDVERIAGNLLSVLSQPLQLSGHECHT
                     TASIGIAMYPADGSDVQTLTKNADMAMYLAKEDGKNGFRFFTKEVKTQSIERLTLESA
                     LRRALERDQFALHYQPKVDMATGQITGVEALLRWTHPELGVLPPAQFIPLAEETGLIV
                     PIGRWVLKEACAQNMAWQRCGLRPVSMAVNLSPRQFVDEHLLQDIDEALAASGMSAVL
                     LQLEVTESMVMRNVSRAVKVLDAIQRRGIR"
BASE COUNT          670 a         1109 c         1024 g          726 t
ORIGIN      
        1 gacgaatgcc ccgacgctgg atggcgtcca gtaccttgac cgcccgggaa acgttgcgca
       61 tcaccatgct ttcggtgacc tcaagctgca gcagcaccgc agacatgccg ctcgccgcca
      121 gcgcctcgtc gatatcctgc aacaagtgct cgtccacgaa ctgcctcggc gacagattga
      181 ccgccatcga taccggccgc aatccgcagc gctgccaggc catgttttgc gcgcaggctt
      241 ccttcaagac ccaacggccg atcgggacga tcaatccggt ctcttcggca agcggtatga
      301 attgagcggg cggcaatacg ccgagctcgg gatgggtcca gcgcaacagc gcctcgacgc
      361 cggttatttg tccagtcgcc atatccacct tcggctgata atgcagcgcg aattgatcgc
      421 gctcgagcgc acggcgcagc gccgattcca gcgtcagccg ctcaatcgac tgggtcttga
      481 cctccttggt gaaaaaccgg aagccgttct tgccgtcttc cttggcgaga tacatcgcca
      541 tgtcggcatt cttggtcagc gtctgcacgt ccgagccgtc cgcagggtac atggcgatgc
      601 cgatgctggc cgtggtgtgg cattcgtggc cgctcagttg caacggctgg ctgaggacgg
      661 acaacaagtt gccggcgatc cgttcgacgt cgtggcgttc cgcggtttcc tccagaatga
      721 caacgaattc gtcgccgccg agccgtgcca ccacatcgct tgaacgcagg gtttggcgca
      781 gccggttggc gatttccacc agcaaaatat cgccggcgtc atgccccagc gaatcgttga
      841 tgaccttgaa cctgtcgaga tcgataaaca gtaccgcgaa tcgccgccgg tggcggcacg
      901 ccgcctcaat cgcgtggtga agcaacccgt tgaacatttc tcgattgggc aggctggtca
      961 ggctgtcgtg cgatgcgagg tattcgatcc gcgcgtccgc cttggttttc tcgtcggcgc
     1021 ggtcgaaatt ctcaagggca aacgacacgt tgtcggccag ccgttgcagg agttcgacga
     1081 attcgggtgt gaaggtgtcc ttttcagcag aaagaaaaag cataacgcca accgcctgcc
     1141 cacggatgag caatggaaat gccgcgcctg actttgctcc atcgccgcga atgatggcgt
     1201 ggaaggcact ggcccgctga tcggccagat aatcgttgct gatgcagggc tgccgcgaac
     1261 ggaacgcagc cccgctcagt ccgcgtccct ccgggcgcgc agcgctgatc gataccttta
     1321 cgtttcgtgt tgtctccgcg gtcggccccg ccacggctgc gatgtcgagg aagtcgctgt
     1381 cgcgattggc tatgctgatg gtggtcgagg tgaatttgcc gccattcgcg gcggcctcgc
     1441 acaccatttc gaacagttgt acccgcgatt tggcccgcat gatcgcttcg ttggtcgcac
     1501 tgagggccgc gaacattcgc gtcagccgtt ccttttgctc ctcggtcttc tgcttttcgt
     1561 cgacgcgatc gaagttatcg agggcgaacg aaacgttttc cgccagtctt ccgagaagct
     1621 ccaccagatc gtcggtgaat gcggcttcat cacaggaaag aaacagaagt acgccgacgg
     1681 ccctgtcgcc gttcttcagc aagggaaagc tcgccccgga tcgtgtcccg tcttctcttg
     1741 ccagggcgtg ccagtgagtg gttcgctcat cggcgagaaa atcattcatg atgcaaggct
     1801 ctcgtgtccg gaacgacgtt ccggtcaaac cccggccttc ggggtccgcg gccgagatcg
     1861 caaagcggcg acttctcatg cggtcgtaat ttatgccttt cgacgcagca attcgaagaa
     1921 actcccccgc gggatccgca agcgcgatgg ttgcggagct gaacgttcct ccgaaaacgg
     1981 cggcttcgca caccagttcg aacaactgtg cccgcgtctt ggcccgcatg atcgcttcat
     2041 tggttgcgct gagggcttca aacatcccgg tcaggcgatc cttttgcttt tcggctttcg
     2101 ctttttcgtc ggctcgatcg aaatttccca gggcgaagga gacattttcc gcgagccgct
     2161 gcagaagttc gacgagttcg ggcgtgaacg ttcccaactc aggtgaattg aaaatgagca
     2221 ctccggccgc ccgatcgccg ttgagtaatg gcagcgccgc gcatgatctg accccactgc
     2281 ggatggcatg gtcgtgccag ggtctggtcc gttcgtcggc caaaaagtcg ttgctgacgc
     2341 aaggctgctg tgcgcggaac gccgacccgg tcaacccacg tccttgcggg agcgactccg
     2401 taatcgcaaa ctccgacagc cgcatctcgc ccgcgcccgg tccggtcgaa gcgacgacac
     2461 gaagatataa cgaatcggac tcggccagag cgacagtcgc ggagccaaac ttcgctccat
     2521 gcaccgctgc ctggcagacc atttcgaaca gctccgcgcg cgacttggcc cgcatgatcg
     2581 cctcgttggt cgcgctaagg gatgcgaaca ttcgcgccag gcgttccttt tgttcatcag
     2641 ctttcgcctt ttcggcggcg cgttcaaaat tgtcgagagc gaacgagacg ttctccgcca
     2701 ttcgcgccag cagcgcgatg atctcttgat ccgccgccca ggatttgctg acaaagaaca
     2761 gcaatacgcc gacgctgttg ccggccttga tgagcggaag cgcgacgcaa gccacaactc
     2821 cggtctcgcg tcccgcctgc tgccacggcc gtccctgcac cgagttgagg atatcgttat
     2881 tgatgcaagg cttctgcgtt cgaaacgcgt tgccgcaaac gccggtccca tggacgtcat
     2941 ccggatcgat cgagaagcgc gcccgcgtga tcagttcgac aatttcaccg gttcccgcga
     3001 ccggtttcag ccagatcgaa cccggctctg caagaaaggc caccgtggca atggattctc
     3061 cgctgttaac ggcggcatcg cagacaagct ggtaaagttc ctgctccgtc ttggcgcgaa
     3121 gaattgcttc gtttgttgcg ctgatggcgc cgaacatccg gttcaatctt cgcatggctc
     3181 gctcgccgct cttgcgcgcg gcctcatgat cgaaattgtc gagcgcacag gaaatgttcg
     3241 ccgacatgcg cacgaaaagc gagacgatct ggtcattcag cgacccggcc tcgcgtcggg
     3301 tgacaagcag cacgccgacg ccgcgaccgt tgcacgtcag cggcaacgcc gccgccgcgc
     3361 cgatatgcgc gtcacatgcc ccttgtcgcc aggcacggga acgcgcgtcg ttcaggtaat
     3421 cattgctgat gcttaccttc tggtcgcgaa aagcctgtcc gcagaccccc gacccctcgg
     3481 gcgtatcagc tacgatcgat atatcgatgc tgcgcaggcg tacaatgtc
//
LOCUS       DASWGM010000830         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2473692,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000830 DASWGM010000000
VERSION     DASWGM010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2473692"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VGA15_22860"
     CDS             complement(<1..757)
                     /locus_tag="VGA15_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HEX9590571.1"
                     /translation="MAEQDDVLHLIDDSGVAPEASTARRWKVAVIDDDQAVHEGTRFA
                     LSDYNLNGQKLEILSAYSAAEGRTLMRAHPDIAAVLLDVIMETDAAGLDLVEYIRNEI
                     KNETVRIILRTGQPGQAPERRVIVQYDINDYKAKTELTADKLFTSLTAALRSYQQLER
                     MVQTRRGLEIIIDAASTLYDFRSMQRLAEGVLTQIASLLNVDCAGILVLRDGGVVGDD
                     FSVLAGSGCYSRFIGSAGSKSLDPDLRSMVEAAF"
     gene            complement(727..>2340)
                     /locus_tag="VGA15_22865"
     CDS             complement(727..>2340)
                     /locus_tag="VGA15_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9590572.1"
                     /translation="TISKTYDEQVLTPARDMAGLYSIIEGATGHRDALIWPSLGKSRE
                     AFTAMLVAANAYYLSLATASAEEARRNTDTIEKTIPVMSDLADNDLQRMALQRLNART
                     VALREGLAKLSEQLASRTELLRNTIDASQADAIGAIDRLSVKMRQHEQKAQETFDRTL
                     ADISRRVLSIAVIFLGIILTVGVLIALSIRLPLQQIMTAMYAITSGDYDRRVQGTDAR
                     DEVGAMARAVEVFRENAIAKRKTEDELRTSKEKAEGALLELNAAQQNLIDAERLAALG
                     GLVAGVAHEVNNPIGISLTVASSFARRAEMFEAELRSDGPLRRSQLEEFVRNSRDAAQ
                     QLVANLHRAGELIQSFKQVAVDRSHAERRQFSLSEATDQIIASLRPVLKKAAIALSVD
                     VPEGLVIDGYPGSYGQILTNLFLNAANHAFADGRSGKISISARARGNDDVEIIFADNG
                     AGMTPDVQRQAFDPFFTTRRNEGGTGLGLHIVYNLVTQQLGGRMMLDSRLGQGTTFRI
                     IMPRAAKGGSTDAASDGTPQWPNRTMSST"
BASE COUNT          388 a          724 c          762 g          466 t
ORIGIN      
        1 tgaaggcggc ttcaaccatc gaccgcaggt cggggtcgag cgacttggat ccggcgctgc
       61 cgatgaagcg gctatagcag cccgaaccgg ccagcacgga aaagtcgtcg ccgaccacgc
      121 cgccgtcgcg cagcaccaga atgccggcgc aatcgacatt cagcagcgac gcgatctgcg
      181 tcagcacgcc ttcggcgagg cgctgcatcg atctgaaatc gtacagggtc gaggcggcat
      241 cgatgatgat ttccagcccg cgtcgggttt gcaccatgcg ttcgagctgc tgatagctgc
      301 gcaacgccgc ggtcagcgag gtaaacagct tgtcggccgt cagctcggtc ttcgccttgt
      361 agtcgttgat gtcgtactgg acgatcacgc ggcgctcggg cgcctggccg ggctgcccgg
      421 tgcgcaggat gatgcgaacg gtctcgttct tgatttcatt gcggatgtat tcgacgagat
      481 cgaggcccgc ggcgtcggtt tccatgatga cgtcgagcag cacggcggcg atatcgggat
      541 gcgcgcgcat caatgtgcgg ccctcggccg ccgaataggc cgagaggatt tcgagcttct
      601 gcccattgag attgtaatcg ctgagcgcaa agcgggtgcc ttcgtgcacg gcctggtcgt
      661 cgtcgatcac ggcgaccttc catctgcgcg cggtggatgc ctccggagcg accccggaat
      721 cgtcgatcag gtggaggaca tcgtcctgtt cggccattgc ggggtcccgt cgcttgctgc
      781 gtctgtggag ccgcccttcg cggccctcgg catgataatg cgaaatgtgg tgccttgtcc
      841 cagtcttgaa tccagcatca tccggccgcc aagctgctgg gtgacgagat tatagacgat
      901 gtgcaggccg agcccggtgc cgccctcgtt gcggcgcgta gtaaagaacg ggtcgaaggc
      961 ctgccgctgc acgtccggcg tcattccggc cccgttgtcg gcaaaaatga tctcgacgtc
     1021 gtcattgccg cgggcccgtg ctgaaatcga gatcttcccg gagcgcccgt cggcgaatgc
     1081 atgatttgcc gcgttgagga acaaattcgt cagaatttgc ccataggaac ccggataacc
     1141 gtcgatcacg aggccctcgg gcacgtcgac cgacagggcg atcgccgcct ttttcaggac
     1201 cggccgcagg ctcgcgatga tctggtcggt ggcctcgctc agactgaact gccggcgttc
     1261 ggcgtgcgag cggtcgaccg cgacctgctt gaaggattgg atcagttccc cggcgcgatg
     1321 caggttcgcc accagttgtt gcgcggcgtc acgcgagttc cggacgaatt cctccagttg
     1381 agaccggcgc aacggaccgt cggatctcaa ttcggcctcg aacatttcgg cgcggcgcgc
     1441 aaaactggac gccacggtca ggctgatgcc gatcgggttg tttacttcgt gggcgacacc
     1501 ggcgaccaga ccgccgagcg ccgccagacg ttcggcgtcg atcaggtttt gctgggcggc
     1561 gttgagttcg agcaaagcgc cttcggcctt ttccttggag gtgcgcaact cgtcttcggt
     1621 cttgcgcttg gcgatggcgt tttcgcggaa cacctccacc gcgcgcgcca tcgcgccgac
     1681 ttcgtccctg gcatcggttc cctgcacgcg ccggtcgtaa tcgcccgatg tgatcgcgta
     1741 catcgccgtc atgatctgct gcaggggcag ccggatactc agcgcgatca ggacgcccac
     1801 ggtgaggatg atgccgagga agatcacggc gatcgacagc accctgcggg atatgtcggc
     1861 cagcgtccga tcgaacgtct cctgcgcctt ttgttcgtgc tgccgcatct tgaccgacaa
     1921 tcgatcgatt gcgccgatgg cgtcggcctg gctggcgtcg atggtgttgc gcaacagctc
     1981 ggtccggctc gcgagctgtt cggataattt cgcgagcccc tcgcgcagcg ccacggtccg
     2041 cgcattcagc cgctgcaggg ccatccgctg caggtcgttg tcggcgaggt cggacatgac
     2101 cgggatggtt ttctcgatcg tgtcggtgtt gcggcgggct tcctcggccg atgccgtcgc
     2161 cagcgaaaga taataggcat tggcggcgac cagcatcgcg gtgaacgcct cgcgcgactt
     2221 tccaagcgac ggccagatca gggcatcgcg gtgccctgtc gcgccctcga tgatcgagta
     2281 aagcccggcc atgtcgcggg ccggggtcaa gacctgctca tcataggtct tcgaaatggt
//
LOCUS       DASWGM010000831         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1189229,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000831 DASWGM010000000
VERSION     DASWGM010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189229"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /gene="treS"
                     /locus_tag="VGA15_22870"
     CDS             complement(<1..1508)
                     /gene="treS"
                     /locus_tag="VGA15_22870"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX9590573.1"
                     /translation="MNLMPSIDENELPVTDLDGDELWYKDAVIYQLHVKAFADSNNDG
                     IGDFAGLTEKLGYLQELGVTALWLLPFYPSPGRDDGYDIADYGAINPDFGTMKDFRRF
                     IVEAKRRGLRVITELVINHTSDQHDWFKRARRSDPDSSARNWYVWSDTDQKYAGTRII
                     FTDTEKSNWTWDPEAGQFYWHRFFSHQPDLNFDNPRVVSALVQVMKRWLDTGVDGFRL
                     DAIPYLCERDGTNNENLPETHAIIRKLRAELDAYAKGKVLLAEANQWPEDVQEYFGQG
                     DECHMAYHFPLMPRIYMALAQEDRFPITDILRQTPDIPNNCQWALFLRNHDELTLEMV
                     TDVERDYLWSTYANDPRARINVGIRRRLAPLMDNDRRKIELMNSLLLSFPGTPIIYYG
                     DEIGMGDNIYLGDRNGVRTPMQWTPDRNGGFSRADPARLYAPTIMDPVYGYESVNVEA
                     QSRSLSSLLSATKRLISVRKSTLAFGRGTMAFIRPVNRSVLAYIRQHGDEVIL"
BASE COUNT          292 a          429 c          486 g          313 t
ORIGIN      
        1 aggatcacct cgtcgccatg ctgcctgata taggcgagca ccgagcgatt gaccgggcgg
       61 atgaacgcca tggtgccgcg gccgaacgca agcgtcgact ttcgcaccga gatcagtcgc
      121 ttggttgcac tgagcaacga agacaggctg cgcgactggg cttcgacatt gaccgactca
      181 tagccgtaga ccggatccat gatggtcggg gcgtaaagcc gtgccggatc ggcgcgggag
      241 aagccgccgt tgcgatccgg cgtccattgc atcggcgtgc gcacgccgtt gcggtcgccg
      301 agatagatat tgtcgcccat gccgatttcg tcgccgtaat agatgatcgg ggtacccgga
      361 aacgacagca gcagcgaatt catcagctcg atcttgcgcc ggtcgttgtc catcagcggc
      421 gccaaccggc ggcgaatgcc gacattgatg cgtgcgcggg gatcgttggc gtaggtcgac
      481 cacaggtagt cgcgctcgac atcggtgacc atttcgagcg tcagttcgtc gtggttgcgc
      541 agaaacagcg cccattggca attgttcggg atatccggcg tctggcgcag gatgtcggta
      601 atcggaaaac gatcctcctg cgcgagcgcc atgtagatcc gcggcatcag cgggaaatga
      661 taggccatgt ggcattcgtc gccctggccg aaatattcct gaacatcctc cggccattga
      721 ttggcttccg ccagcagcac cttgcccttg gcataggcgt cgagttcggc gcgcagcttc
      781 ctgatgatgg cgtgggtttc cggcaggttc tcgttattgg tgccatcgcg ctcgcacaga
      841 taggggatgg catcgagacg gaaaccatcg acgccggtat cgagccagcg cttcatcacc
      901 tgcacgagag cgctgaccac gcgcggattg tcgaaattga gatccggctg gtgcgagaag
      961 aagcggtgcc agtagaattg gccggcttcc gggtcccatg tccagttcga cttctcggtg
     1021 tcggtgaaga taatccgcgt gccggcatat ttctggtcgg tatcgctcca cacataccag
     1081 ttgcgcgcgc tcgagtcagg atcgctgcgg cgggcgcgct tgaaccagtc gtgctggtcg
     1141 gaagtatgat tgatgacaag ttcggtgatg acccgcagac cgcgccgctt ggcttcgacg
     1201 atgaagcggc gaaaatcctt catggtgccg aagtcgggat tgatggcgcc gtagtcggcg
     1261 atgtcatagc cgtcatcgcg gccgggcgac gggtagaacg gcaacagcca cagcgcggtg
     1321 acgccgagtt cctgcagata gccgagcttc tcggtcagtc ccgcgaaatc gccgatgccg
     1381 tcattattgc tgtcggcaaa ggctttgacg tgaagctgat agatgacggc gtctttgtac
     1441 cagagctcat cgccatcgag gtcggtgacc ggcaactcgt tttcgtcgat ggatggcatc
     1501 aggttcatgg cgtccctcac
//
LOCUS       DASWGM010000832         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_776661,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000832 DASWGM010000000
VERSION     DASWGM010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776661"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..>1707)
                     /locus_tag="VGA15_22875"
     CDS             complement(277..>1707)
                     /locus_tag="VGA15_22875"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9590574.1"
                     /translation="TSNIHYEHSDRIRGLASGGIGAMHQLAQHVGLTEAIDQQVEVLK
                     VHLPYHESDHVLGIAYNLLCGGTCLQDIERRRQDEVYLDALNAQRIPDPTTAGDFCRR
                     FTEADIEALQAAINDTRLRVWRAQPAAFFDEAIIDADGTLAETTGQCKEGIDIAYNGV
                     WGYHPLVVSLANTQEPLYLVNRSGNRPSYEGAAERFDQACTLCQAAGFRRILFRGDTD
                     FTQTRHLDRWDAGGVRFVFGCDARANLIAAADALPRRAWTPLVRRPPYAVQTEPRERP
                     ANVKAAIIVAREFKNLRLEAEAVAEFPYQPGACTKPYRMVVVRKNISVEQGDHRLFDD
                     LRYFFYLTNDQDTPAADVVFLANDRCNQENLIDQLKRVGATRMPVDTLLSNWAYMVMA
                     ALAWTLKAWFALRLPATGRWATRYAAEKAAVLRMEFKAFLHAFMLMPVQVVRTSRRLV
                     FRLLAWNPWQAVFLRGFDQLRQPLRS"
BASE COUNT          302 a          576 c          521 g          308 t
ORIGIN      
        1 cgttcatcgc cagctccgag gcgttggtgg ctgaggccaa cgtccggctt cccgatgctc
       61 gatgaacgtc tacccccttc tatctagccc caaaataagc gcgtttgcgg atcggccgat
      121 gacgtgaagc gccatgagat cgcggcgcag agttcccctg tcgggacgct gtcccattgc
      181 cctcgccgtt cgattgtcgt catcggttga tcctccccac gtgcgacgga cggttcagta
      241 catccgggcc tcgctatccc ggtctcggcg gtcgactcac gaccggagcg gctgacgcag
      301 ttggtcgaac ccgcgcagga agacggcctg ccacggattc cacgcgagca accggaagac
      361 gagccggcgg ctcgtgcgca cgacctgtac cggcatgagc ataaaggcgt ggaggaatgc
      421 tttgaattcc atgcgcagga ccgcagcctt ctcggcggcg taacgtgtcg cccaccgacc
      481 cgtcgcaggc aaccgcaacg cgaaccatgc cttgagcgtc cacgccagcg ccgccatgac
      541 catataggcc cagttactca gcaacgtgtc gacgggcatg cgtgtcgcgc cgacgcgctt
      601 gagttgatcg atcagattct cctggttgca ccggtcgttg gccaagaaca ccacgtcggc
      661 ggccggcgtg tcctgatcat tggtcagata gaagaagtac cgaaggtcat cgaacagccg
      721 gtggtccccc tgttcgaccg aaatattctt gcgcacgacg accatgcggt acggcttcgt
      781 gcaggcgccc ggttgatagg ggaactcggc gacggcttcc gcctccaacc ggaggttctt
      841 gaactcccgc gcgacgatga tggccgcttt cacgttcgcg gggcgctcgc gcggctcggt
      901 ctggaccgcg tacggcgggc gccgcaccaa tggcgtccac gcgcgccgcg gcagcgcgtc
      961 ggccgcggcg atcagattcg cgcgggcatc gcagccgaac acgaaccgga ccccgcccgc
     1021 gtcccaccga tccagatggc gcgtctgcgt gaaatccgta tccccccgga agaggatgcg
     1081 ccgaaacccc gccgcctgac acagggtgca ggcttgatcg aatcgttcgg ccgcgccctc
     1141 gtacgacggg cgattcccgc tccggttcac cagatagagc ggctcctgcg tattcgccag
     1201 cgagacgact aacgggtgat agccccagac gccgttgtag gcaatatcga tcccttcctt
     1261 acactgcccg gtcgtctcag ccagtgtgcc gtcggcgtcg atgatcgctt cgtcgaaaaa
     1321 cgccgctggc tgcgcgcgcc agacccgcag ccgcgtgtcg ttgatcgcgg cctgcaacgc
     1381 ctcgatgtcg gcctcggtga accggcggca gaaatcgccg gcggtggtgg gatcggggat
     1441 ccgttgcgca ttcagcgcat cgagatacac ctcgtcctgc cggcgccgct cgatgtcttg
     1501 caaacacgtc ccgccgcaca acaggttgta ggcgatcccc aacacgtgat cggattcgtg
     1561 atacggcagg tgcaccttta agacctcgac ctgctgatcg atcgcctcgg tcaagccgac
     1621 gtgctgagcc agctgatgca tcgccccgat gcccccactc gccagcccac ggatgcggtc
     1681 actgtgctca tagtgaatgt tgctggt
//
LOCUS       DASWGM010000833         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2707007,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000833 DASWGM010000000
VERSION     DASWGM010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2707007"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..2347)
                     /locus_tag="VGA15_22880"
     CDS             complement(71..2347)
                     /locus_tag="VGA15_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9590575.1"
                     /translation="MTSFAFRRDIVTKPIFSWARGVLPSMSDTEREALEAGDVWWDAD
                     LFTGNPDWSKLLATVPATLTPEETAFLHGPVDELCAMLDDWKINWEWRDLPGEVWDFI
                     KREKFFGMIIPKEYGGLGFSPYAHSEVVRKISSRSLTAAVTVMVPNSLGPGELLMRFG
                     TKDQQQQWLPRLADGREIPCFGLTSPEAGSDAASMIDSGILCKGNFEGCEVLGLRLNW
                     HKRYITLGPVATLLGLAFKAYDPDHLVGSQDELGITVALIPTHLPGVEIGHRHLPSMQ
                     VFQNGPNRGRDVFIPMDYIIGGQERLGQGWKMLMTALAAGRGISLPSLSAAGAAYAAR
                     TTGAYARIREQFNVPIGKFEGIEEPLARIAGTAYLLDAARRLTCAALNQGRHPAVISG
                     IMKLHATERMRIAIDDAMDIHGGKAVIDGPQNYMGNLYRSVPVGITVEGANILTRNLI
                     VFGQGAIRAHPYLLEEMNALGDPDRAKGLDAFDKAFWKHIGHSITTMFRAWGRSWSGG
                     AFAPAPDAGDATKFYRQLSRYSSAFALCADMALLTLGGALKRKEMLSARFGDILSELY
                     LLSAALKRWQDEGRQGADFAALEWCMASGFRTIENRFAEILANLPNRFVAVVLKFLIQ
                     PFGARVLGPSDIVIHRCAQLVLEPSAARDRLTPDLSHIDDDRGVARLERAFLLVTGAE
                     DIAKRMRAAHVHDWKEAVKKGIITQAEGEQLAAAHEAAAKVIEVDDFAPEALSPIYKK
                     PAEVHQFLQGLGETRAAS"
BASE COUNT          454 a          755 c          738 g          409 t
ORIGIN      
        1 gggcccggcc cgctgcgcgc tttcaggaac ggcgtccggc tgccgtcgat gataaaaacc
       61 ggtcgcgcca tcagctcgca gcccttgtct cacccagacc ttgcaagaac tgatgcactt
      121 cggcgggctt cttgtaaatc ggcgacagcg cctccggcgc aaagtcgtca acttcaatga
      181 ctttggcggc cgcctcatgc gcggcggcga gttgctcgcc ctcggcttgc gtaatgatgc
      241 ccttcttcac cgcctctttc cagtcgtgga cgtgggcggc gcgcattctt ttggcgatat
      301 cttccgcgcc ggtgacgagc aaaaatgccc tctccagccg cgcgacaccg cgatcgtcat
      361 cgatgtgcga cagatccggc gtcaggcgat cgcgcgccgc cgacggctcc agcaccagct
      421 gcgcgcagcg gtggatcacg atgtcggacg gaccgagcac gcgggcgccg aacggctgga
      481 tcaggaattt cagcacgacg gcgacaaacc gattgggcag gttcgcgagg atttcggcaa
      541 aacgattttc gatggtccta aagccgctcg ccatgcacca ttcgagcgcg gcaaaatccg
      601 caccctgacg gccctcgtcc tgccagcgtt tcagcgcggc ggacagcaaa tagagttcgg
      661 aaaggatatc gccgaaccgt gccgacagca tttccttgcg cttcagcgcg ccgcccagcg
      721 tcaacagcgc catgtcggcg cacagcgcga acgccgacga atagcgcgag agctggcgat
      781 agaatttagt ggcgtctccg gcgtcgggcg ccggggcgaa cgcgccaccg ctccagcttc
      841 ggccccaggc gcggaacatg gtcgtgatgc tgtgaccgat atgtttccag aacgccttgt
      901 cgaaagcgtc gagacccttg gcacggtcgg gatcgccgag cgcgttcatc tcttccagca
      961 gataaggatg cgcgcggatc gcaccctgcc cgaacacaat aagattgcgg gtcaggatat
     1021 tggcgccctc gacggtgatg ccgaccggca ccgagcgata gagattgccc atgtaattct
     1081 gcggaccgtc gatcacggcc ttgccgccat ggatgtccat ggcgtcgtcg atcgcgatcc
     1141 gcatccgctc ggtggcatgc agcttcatga tgccggaaat gaccgcggga tgacgaccct
     1201 gattgagcgc ggcgcaggtc agccgccgcg ccgcatcgag caggtacgcc gtgccggcga
     1261 tgcgggccag cggctcctcg atgccttcga atttgccgat gggcacgttg aactgctcgc
     1321 ggatccgcgc ataggcgccg gtggtgcgcg cggcataggc cgcgcccgcg gccgacaatg
     1381 acggcagcga gatgccgcgg ccggcggcca gcgccgtcat cagcatcttc cagccctggc
     1441 cgagacgttc ctggccgccg atgatatagt ccatcggaat gaagacatcg cggccccggt
     1501 tcggaccgtt ctggaacacc tgcattgacg gcagatggcg atggccgatc tcgacgccag
     1561 gcagatgggt cgggatcagc gccacggtaa tgccgagctc atcttgagat ccgaccagat
     1621 gatcggggtc ataggccttg aaggcaaggc cgagcaatgt agcgaccggg ccgagcgtga
     1681 tatagcgctt gtgccagttg agacgcaggc caagcacttc gcagccctcg aaattgccct
     1741 tgcagaggat gccgctgtcg atcatcgacg cggcgtcgga gccggcttcc ggactggtca
     1801 agccaaagca gggaatctcg cgtccgtcgg caaggcgcgg cagccattgc tgctgctgat
     1861 ccttggtgcc gaagcgcatc aaaagttcgc ccggccccag cgagttcggc accatcaccg
     1921 tgacggcggc ggtcagcgaa cgcgacgaaa tcttgcgcac cacttcggaa tgcgcatagg
     1981 gcgagaagcc gagcccgcca tactctttcg gaatgatcat gccgaagaat ttttcgcgct
     2041 tgataaagtc ccagacctcg cccggcagat cacgccattc ccaattaatc ttccagtcat
     2101 cgagcatcgc gcagagttca tcgaccggac cgtggagaaa cgccgtttcc tccggcgtca
     2161 gggtcgcagg caccgtggcg agcagcttcg accagtcggg attgccggtg aacagatccg
     2221 cgtcccacca gacatcgccg gcctcgagcg cctcgcgctc ggtgtccgac atcgacggca
     2281 gcaccccgcg tgcccaggag aagatcggct tggtgacgat atcgcggcga aaggcaaagg
     2341 aggtcatggc gatgtc
//
LOCUS       DASWGM010000834         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1785132,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000834 DASWGM010000000
VERSION     DASWGM010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785132"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..586
                     /locus_tag="VGA15_22885"
     CDS             2..586
                     /locus_tag="VGA15_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9590576.1"
                     /translation="MNHDAYADNYIREILSGVKSIAMVGASPVNVRPSYFAFKYLAQR
                     GYDMIPVNPGHVGKSLLGKPFVASLRDIGHPIDMIDIFRNSSHIMPVVDEALELVPPP
                     KIIWMQLGARDDAAAAKAEAAGLKVVMNRCPKIEYGRLSSEISWMGVNSRTLSAKRAP
                     MPTQGMRLSLNRMSVGGGETAASDRAAKNKNDLA"
     gene            814..2094
                     /locus_tag="VGA15_22890"
     CDS             814..2094
                     /locus_tag="VGA15_22890"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX9590577.1"
                     /translation="MTDRLPGFSTLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHA
                     ASLFGLQAFGNIYTRIGNPTQAVLEERLAALEGGTAALAVASGHAAQVVVLQQLLMPG
                     DEFIAARKLYGGSINQFNHAFKSFGWNVVWADPDDIGSFERAVTPHTKAIFIESIANP
                     SGSVTDIEAISAVARKAGVPLIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSL
                     GGIIVDAGTFDWSKDNKYPMLSEPRPEYHGIKLQETFGNFAFAIACRVLGLRDLGPAI
                     SPFNAFLILTGIETLPLRMQKHCENAKSVAEWLLAHPAVAAVNYAGLSGDKYNNLARK
                     YAPKGAGAVFTFSLKGGYEAGVNLVSKLQLFSHLANVGDTRSLVIHPASTTHSQLDDA
                     AKIKSGAAPDVVRLSIGIEDKEDLIADLAQALSA"
     gene            complement(2189..>2477)
                     /locus_tag="VGA15_22895"
     CDS             complement(2189..>2477)
                     /locus_tag="VGA15_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590578.1"
                     /translation="AEQSVDDGGKQARSPGRARSKPLKPLRGECRAFSGVTVVTNARV
                     YYTPRAAAGASGARHSLRPLIGEGGKFRAKLARVKRRDREAVCIEVVCIAV"
BASE COUNT          497 a          777 c          735 g          468 t
ORIGIN      
        1 gatgaaccat gacgcctacg ccgacaatta catccgcgaa attctgagcg gcgtgaaatc
       61 gatcgcgatg gtcggggcgt cgccggtcaa tgtgcggccg agctactttg ctttcaaata
      121 tctggcgcag cgcggctacg acatgatccc ggtcaatccc ggtcatgtcg gcaagagcct
      181 gctcggaaaa cccttcgtcg cgtcgctgcg cgatatcggc catcccatcg acatgatcga
      241 catcttcagg aactcaagcc acatcatgcc ggtggtcgat gaggcgctgg aactcgtgcc
      301 gccgccgaaa ataatctgga tgcagcttgg cgcgcgcgac gatgcggcgg ccgcaaaggc
      361 ggaagccgcc ggtctcaagg tcgtcatgaa ccgctgtccc aagattgagt acggacggct
      421 gtcctcggaa atttcgtgga tgggcgtcaa ttcgcgaacg ctgagcgcaa agcgcgcgcc
      481 gatgccgacc cagggcatgc ggctttcatt gaaccggatg agcgtcggcg gcggcgagac
      541 cgcggcgtcc gatcgcgcgg cgaaaaacaa gaacgacctt gcgtgacgcg aatacgaaac
      601 gattctttca agaatatggc gacgcaaagc gcggccgttc ccaacacgat gtgaggcacc
      661 gcgccggctt gacggcggac gccgctgacg cgattgtcga ttattcggac gaggcgggct
      721 tgcgccgtct cgaccataag atcgcaccaa attgctcatc cttcgagacg cggccgcacg
      781 gctctcgatg aggccttcat aagggaaaaa acaatgaccg atcgtcttcc gggattttcg
      841 accctcgccg tgcatgccgg cgcgcagccc gatcccacta ccggagcgcg ggcgacgccg
      901 atctatcaga ccacgtcgtt cgtgttcaac gacgccgacc atgccgcgtc gctgttcggg
      961 ctgcaggcat tcggaaacat ttacacccgg atcggaaatc cgacccaggc cgtgctggaa
     1021 gaacgccttg ccgcgcttga gggaggtacc gccgcactcg cggtcgcatc gggacatgcc
     1081 gcacaggtcg tcgtgctgca gcaattgctc atgccgggag acgaattcat cgcggcgcga
     1141 aaactctatg gcggttcgat caaccagttc aaccacgcct tcaagagttt tggctggaac
     1201 gtggtgtggg ccgatcccga tgatatcggc agcttcgagc gcgcggtgac gccgcatacc
     1261 aaggcgatct tcatcgagtc gatcgccaac cccagcggca gcgtcaccga catcgaggcg
     1321 atctccgccg tcgcccgcaa ggccggcgtg cccttgattg tcgacaatac gctggcgact
     1381 ccctatctga ttcgcccgat cgatcatggc gccgacatcg tcgtgcattc gctgacgaaa
     1441 tttctcggcg gccacggcaa ttcgctcggc ggcatcatcg tcgacgccgg caccttcgac
     1501 tggtcgaagg acaacaaata tccgatgctg agcgagccgc gtcccgaata tcacggcatc
     1561 aagcttcagg agacgtttgg caatttcgca ttcgccattg cctgccgcgt gctgggcttg
     1621 cgcgatctcg gtccggcgat ctcgccgttc aacgcgtttc tcattctcac cggcatcgaa
     1681 acgttgccgc tgcggatgca gaagcattgc gagaatgcga aatcggtcgc ggagtggctg
     1741 ctcgcacatc cagccgtggc ggcggtcaat tacgccgggc tgtccgggga taaatacaac
     1801 aacctcgccc gtaaatacgc gccgaagggc gccggcgcgg tgttcacctt cagcctcaag
     1861 ggcggctatg aggccggcgt caatctggtg tcgaagctgc aactgttctc gcatcttgcc
     1921 aatgtcggcg acacccgctc gctggtgatt cacccggctt ccaccacgca cagccagctc
     1981 gacgatgctg ccaagatcaa gtcgggcgca gcacccgacg tggtgcggct gtcgatcggc
     2041 atcgaggaca aggaagattt gatcgccgat ctggcgcagg cgctgagcgc gtaatggacg
     2101 tagcccggat gagcgaagcg atatccggga acgaacgtaa cggtggcccc ggatgtcgct
     2161 tcgctcatct gggctgcgga ttcgattttc aaacagcaat acacacaact tcaatacaca
     2221 cagcttcgcg atctcgccgc ttcacccgcg cgagttttgc tctgaacttc ccgccctctc
     2281 cgatgagagg gcgcagggaa tgccgggcgc ccgatgcgcc cgcagccgcg cgtggtgtgt
     2341 agtaaacacg cgcgttagtc accacggtca caccggaaaa cgcccggcat tccccgcgca
     2401 atggttttaa cggtttgctt cgtgctctcc ccggtgaccg ggcttgtttg ccaccgtcat
     2461 cgacggattg ctccgcc
//
LOCUS       DASWGM010000835         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1190285,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000835 DASWGM010000000
VERSION     DASWGM010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190285"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGA15_22900"
     CDS             <1..361
                     /locus_tag="VGA15_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEX9590579.1"
                     /translation="IPITGGDVRGDINGKVCAFGADFQIIRPNELIELEAKYAFETDD
                     GAVVYVENKGVRFGPIELLQRLKRGEPVDPKLIYFRTVPKFETGAEKYRWLMQNLFIG
                     SAARHVDRVVIDVHQVL"
     gene            646..1449
                     /locus_tag="VGA15_22905"
     CDS             646..1449
                     /locus_tag="VGA15_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9590580.1"
                     /translation="MGNAAPTAIADHSALLRIETVGPVLTVGLNRPAKRNALNDGIIL
                     AIQDCFSDLPEGIGAVVIHGIGDHFSSGLDLSELTEHDATEGLRHSQMWHRVFDRIQY
                     SRVPVIAALKGAVIGGGLELACAAHIRVAEPSAYFALPEGQRGIFVGGGGSVRLPRLI
                     GVARMTDMMLTGRVYSATEGASYGFAQYLTEEGHAYPKAMELAAKVAQNAPLTNFAVL
                     QALPMIAEANPQTGLLMESLMATVAQSDKEAKLRIRAFLDRKTAKVKPT"
     gene            1451..>2619
                     /locus_tag="VGA15_22910"
     CDS             1451..>2619
                     /locus_tag="VGA15_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9590581.1"
                     /translation="MSAKPNMSVPAGSSEAYPLRAISFGDPAVSIDRRTDGTIYLRPK
                     IALGEYPDRLTDRLHHWAKAEPNRIFMAERDKGGVWRQITYAQLLGFSRHIASALLAR
                     GLSADRPIVILSGNSIDHALVAFGALYAGIPFCPVSPAYSLISKDYGKLGYLMKLLTP
                     GLVFADDASKFADALAANVPTEVEILASHGDVPGRQVTMLSELLATPEHPGLDAAHDA
                     IGADTIAKFLLTSGSTGHPKAVINTQRMICANQVMLRETLAFLKDEPPVIVDWLPWNH
                     TFGGNHNIGLTLFNGGSMYLDEGKPMPGGIEETVRNLREISPTVYFNVPKGYEALLPY
                     LRDDSALRAKFFHRLHAMFFSGAALSPFIWNSLDELAVQETGVRVPMLTGLGATET"
BASE COUNT          478 a          818 c          807 g          516 t
ORIGIN      
        1 cattccgatt accggtggcg acgtgcgcgg cgatatcaac ggcaaggtct gcgcgttcgg
       61 cgcggacttc cagatcatcc ggcccaatga gctgatcgaa ctggaagcga aatatgcctt
      121 cgagaccgac gacggcgcgg tcgtctatgt cgagaacaag ggcgttcgtt tcggccccat
      181 cgagttgctg cagcggctga agcgcggcga gcccgtcgat cccaagctga tctatttccg
      241 cactgtgccg aagttcgaaa ccggcgccga aaaataccgc tggctgatgc agaacctgtt
      301 catcggctcc gccgcccgcc acgtcgaccg cgtcgtgatc gacgtgcatc aggtgctgta
      361 gttacggggc gccgagggcc tttccgccaa cctgcacgga cgctgccatt gcgccgcaaa
      421 ccacgtccct gcgagtcgtc ccggcgaagg ccgggaccca tacggcgcgg actgacgtct
      481 tctgcagtgt gttcgatgcc ttctgcaaca atcgatgcca gggattatgg gtcccggcct
      541 tcgccgggac gacgctccgg actttattgg acgcggagct tatcgattga ctccccctca
      601 aatcgttata aaacataact attctggaca ggacataata ggcctatggg aaacgccgca
      661 cccaccgcga tcgccgatca ctccgcgctg ctcaggatcg agacggtcgg cccggtgctg
      721 acggtgggcc tcaaccgccc ggccaaacgc aacgcgctga atgacggcat tatcctcgcg
      781 atccaggact gtttctctga tctgccggag gggatcggtg ccgtggtgat ccatggcatc
      841 ggcgatcatt tttcatcggg gctggatctg tcggagctga ccgagcacga tgccaccgaa
      901 ggtttgcggc attcgcagat gtggcaccgg gtgttcgacc gcattcaata tagtcgcgtg
      961 cccgtgatcg ccgcgctcaa gggtgccgtg atcggcgggg ggctggagct tgcctgcgcc
     1021 gcgcacattc gtgtcgccga accctcggcc tatttcgcgc tgcctgaggg ccagcgcggc
     1081 atcttcgttg gcggtggcgg ctcggtgcgg ctgccgcggc tcatcggtgt cgcgcggatg
     1141 acggatatga tgctgaccgg gcgggtttat tccgcgaccg agggtgcgtc ctatggcttt
     1201 gcgcaatatc tcaccgagga aggccatgcg tatcccaagg cgatggaact cgccgccaag
     1261 gtcgcgcaga atgcgccgct gacgaatttc gcggtgctgc aggcactgcc gatgatcgcg
     1321 gaggctaatc cgcaaaccgg ccttttgatg gaatcgctga tggcgacggt ggcgcagagc
     1381 gacaaggaag caaagcttcg gatccgcgcg ttcctcgatc gcaagaccgc aaaggtgaag
     1441 ccgacctgac atgagtgcga aaccgaacat gtctgtcccg gccggaagca gcgaagccta
     1501 tccgctgcgc gcaatctcgt tcggcgatcc tgctgtgtca atcgatcggc gcaccgacgg
     1561 cacgatctat cttcgtccca aaattgcgct cggcgaatat cctgaccgac tgaccgaccg
     1621 cctgcaccat tgggccaagg cggagccgaa ccgcattttc atggcggagc gcgacaaagg
     1681 tggcgtgtgg cggcaaatca cttacgcgca attgcttggc ttctcccgac acatcgcttc
     1741 cgcgctgctc gcgcgcggtc tctcggccga cagaccgatc gtcatcctct ccggcaattc
     1801 gatcgaccac gcattggtgg cgttcggcgc gctctatgcg ggcatcccgt tctgcccggt
     1861 gtcgccggcc tattcgttga tttcgaagga ttacggcaag ctcggctatc tgatgaagct
     1921 gctgacaccc ggtctggtgt tcgccgacga cgccagcaaa tttgccgatg cgctcgccgc
     1981 caatgtcccg accgaggtcg agatcctggc ctcgcacggc gacgtgcccg gtcgccaggt
     2041 gacgatgctg agcgagttgc tggcgacgcc ggagcatccc ggcctcgatg cggcccatga
     2101 cgcgatcggc gccgatacca ttgcaaaatt cttgctgacc tcgggctcga ccggccatcc
     2161 gaaggcggtc atcaacaccc agcggatgat ctgcgccaat caggtgatgc tgcgcgagac
     2221 gctggccttc ctgaaggacg agccgcccgt gatcgtcgac tggctgccgt ggaatcacac
     2281 ctttggcggc aatcacaata tcgggctgac gctgttcaac ggcggctcga tgtatcttga
     2341 cgagggcaag ccgatgcccg gcggcatcga ggaaaccgtg cgcaatctcc gcgaaatttc
     2401 gcccacggtc tatttcaacg tgcccaaggg gtatgaagcg ctgctgccgt atttgcgcga
     2461 cgattccgcg ttgcgcgcaa agttttttca tcggctgcac gcgatgttct tcagcggtgc
     2521 ggcgctgtcg ccctttatct ggaacagcct cgacgaactc gcggtgcagg aaaccggcgt
     2581 tcgggtgcct atgctgaccg gcctcggcgc caccgagac
//
LOCUS       DASWGM010000836         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_372095,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000836 DASWGM010000000
VERSION     DASWGM010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372095"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VGA15_22915"
     CDS             <1..138
                     /locus_tag="VGA15_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEX9590582.1"
                     /translation="VKYPNVNSLVEMTDMREAQRSYEANLNIISATRRMIQRTLDILK
                     A"
     gene            155..466
                     /gene="fliE"
                     /locus_tag="VGA15_22920"
     CDS             155..466
                     /gene="fliE"
                     /locus_tag="VGA15_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX9590583.1"
                     /translation="MASPTVAANAYASLARIMDTGGAGKGSEAGGGQSFGALLKDAIG
                     SVMDAGRKSDAQTVAMASGKANVMDVVTAVAETDVAVSTLVSVRDRVIQSYEDIMKMP
                     I"
     gene            614..877
                     /gene="fliQ"
                     /locus_tag="VGA15_22925"
     CDS             614..877
                     /gene="fliQ"
                     /locus_tag="VGA15_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX9590584.1"
                     /translation="MTGAETLDVARDAIWTIVVVSSPLMIVGLVVGVVVSLFQALTQI
                     QEQTLVFVPKILAIFVTLLLALPFMADSLHSHMMRISSRIIGG"
     gene            895..1665
                     /gene="fliR"
                     /locus_tag="VGA15_22930"
     CDS             895..1665
                     /gene="fliR"
                     /locus_tag="VGA15_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX9590585.1"
                     /translation="MRIDVSLLPALAAAFMLAFARIGAMVMLLPGLGETNIPVRVKLG
                     IALLLTLVILPLHRAAYHIDMQSITPLLVLMLHEIVIGVVLGATARVTLSALQVAGSV
                     IAQQLGLGFVTAIDPTQGQQGLLIGNFLTILGLTLLFATDSHHLVIAALNDSYSIFSP
                     GEMMPSGDVAALATRAFAAAFRIGLQLSAPFLVFGLVFNIGLGVLARLMPQMQVYFVG
                     VPLSILAGFLIFALVLTAMMGTFLDYFVGVMHDLIPLS"
     gene            1678..>2687
                     /locus_tag="VGA15_22935"
     CDS             1678..>2687
                     /locus_tag="VGA15_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HEX9590586.1"
                     /translation="MSDDNDASDKTEDPTQKRLDDAHDRGDVAKSQEINTWFIIAGAT
                     LVLSSFSGSIGSGLLMPMRNLIANSWMVHADGPGLLQLAQSLEYVLIAALGVPLLMLA
                     LAAIAGNMIQHRLVWSGESLKPKFSKVSPGAGLKRIFGKQAAANFGKGLFKLAALGAV
                     MMAILWPERHRLESMVRFDPAAVLGATTTLTLQLMGAVVAMLAIVAIADYFFQYRQWH
                     ERQKMSLQEMKKEFKQSEGDPHIKGRIRQLRHARMKKRMMSAVPKASVIITNPTHYAV
                     ALSYERGMPAPICPAKGIDSIALKIREIAKAHDIPIVENVPLARALHATVKVDEEIPV
                     EH"
BASE COUNT          510 a          798 c          832 g          547 t
ORIGIN      
        1 gtcaaatatc ccaacgtcaa ttcgctggtt gaaatgaccg acatgcgcga agcgcagcga
       61 tcctacgagg cgaacctcaa catcatcagc gccacgcgcc ggatgattca acgcacgctc
      121 gacatcctca aggcctgaac aggaaacctg aaccatggct tcacccacag tcgctgccaa
      181 tgcctacgcc agcctcgccc gcatcatgga caccggcgga gccggcaaag gttccgaagc
      241 gggcggcggc caatccttcg gagcactgct caaggacgcg atcggaagcg tcatggacgc
      301 cggaaggaaa tccgacgcgc agaccgtcgc gatggcgtca ggcaaggcca acgtcatgga
      361 cgtggtgacg gcggtggcgg aaaccgacgt cgcggtctcg acgctggtgt cggtgcgcga
      421 ccgcgtcatc cagtcctacg aagacatcat gaagatgccg atctgatcgt cattgcgagc
      481 caacgggtcg gcgcaaagcg ccgcccgatg acaggcttcg cgaagcaatc catctctcgg
      541 aatggattgc ttcgtcgcaa gtgctcctcg caatgacggg tgtaaatgct tcgagaatag
      601 acaggacatt gcaatgaccg gtgctgaaac cctcgacgtg gcgcgcgatg cgatctggac
      661 catcgtcgtg gtgtcgtcgc cgctgatgat cgtcggtctc gtcgtcggcg tcgtggtgtc
      721 gctgtttcag gcgctgacgc agatccagga acagacgctg gtgttcgtgc cgaaaatcct
      781 ggctatcttc gttaccctgt tgcttgcgct accatttatg gcggactcgc tgcacagtca
      841 catgatgcgg atatcgtcgc gaatcatagg cggttgatgc actatgcgcg gatcatgcgg
      901 atcgatgttt ccctgctgcc ggcgctcgcc gccgccttca tgctggcctt cgcccgcatc
      961 ggcgcgatgg tgatgctgtt gccggggttg ggcgagacca atattccggt tcgtgtcaaa
     1021 ctcgggatcg cgctgctgct gacgctggtg attttgccgc tgcaccgcgc cgcctatcac
     1081 atcgacatgc agtcgatcac gccgcttttg gtattgatgc tgcatgagat cgtgatcggg
     1141 gtcgtgctcg gcgccaccgc gcgggtgacg ctctcggcgc tgcaggtcgc aggttcggtg
     1201 atcgcccagc aactcggcct cggctttgtc accgcgatcg atccgaccca gggtcagcag
     1261 ggcctgctga tcggaaattt cctcaccatc ctcggcctga cgctgttgtt tgcgaccgac
     1321 agccatcatc tcgtcatcgc agccctcaac gacagctatt cgattttttc gccgggagaa
     1381 atgatgccga gcggtgacgt cgcggcgctg gccacccgcg ccttcgccgc cgcgtttcgg
     1441 atcggcctgc agctttcggc gccgtttctg gtgttcggac tggttttcaa tatcgggctt
     1501 ggcgtgctgg cgcggctgat gccgcagatg caggtttatt ttgtcggcgt accgctgtcg
     1561 attttggccg ggttcctcat tttcgccctt gtcctcacgg cgatgatggg aacgtttctg
     1621 gattatttcg tcggcgtcat gcacgatctg attccgttga gctgaaggcc caaaaccatg
     1681 tccgatgaca acgacgcatc cgataaaaca gaagacccga cacaaaaacg tctggatgac
     1741 gcccacgacc gcggcgatgt tgcgaaaagc caggagatca acacctggtt cattatcgcc
     1801 ggtgcgacac tggtactgtc gtcattttcc ggatcgatcg gcagcggcct tctgatgccg
     1861 atgcgcaacc tgatcgccaa ttcctggatg gtccacgccg acgggcccgg ccttttgcag
     1921 ctggcgcaga gtctcgaata tgtgctgatc gcagcactcg gcgtgccgct gttgatgctg
     1981 gcgctggcgg cgatcgcggg taacatgatc cagcaccggc tggtatggtc gggcgagtcc
     2041 ctcaagccga agttcagcaa ggtctcgccc ggggccggac tgaagcggat cttcggcaag
     2101 caggcggcgg cgaatttcgg caagggcctt ttcaaactgg ccgcgctggg cgcggtgatg
     2161 atggcgattc tctggccgga gcgtcaccgg ctggaatcga tggtgaggtt cgacccggcg
     2221 gcggttttgg gcgcgaccac caccctgacg ctgcaattga tgggcgccgt ggtggcgatg
     2281 ctcgcgattg tcgcgatcgc ggactatttc ttccagtacc gccaatggca tgagcggcag
     2341 aagatgtcgc tgcaggaaat gaagaaggag ttcaagcaat ccgaaggcga cccgcacatc
     2401 aagggccgga tcaggcagtt gcgccacgcg cggatgaaga agcgcatgat gtccgcggtt
     2461 cccaaggcct cggtgattat caccaacccg acccactatg cggtggcgct gagttacgaa
     2521 cgcggcatgc cggcgccgat ctgtcccgcc aagggcatcg acagcatcgc gctcaagatc
     2581 agggaaatcg ccaaggcgca cgatatcccg atcgtcgaga acgtgccgct ggcgagggct
     2641 ctgcatgcga ccgtgaaggt cgacgaggag atcccggtgg agcacta
//
LOCUS       DASWGM010000837         4321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_639839,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000837 DASWGM010000000
VERSION     DASWGM010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639839"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VGA15_22940"
     CDS             <1..412
                     /locus_tag="VGA15_22940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9590587.1"
                     /translation="LFSEDRLMLVTARRGDLAGRRQIDFQEVRDRDFVGLTNSTALQI
                     HIARHAARLGLRLRFRARLRDFDAICRMVAAEVGIAVVPEAAAKRCARSMPIAMIRIR
                     DPWANRKLTICARSFKSLPRPAKQLVEHLRKAAQ"
     gene            complement(861..1829)
                     /locus_tag="VGA15_22945"
     CDS             complement(861..1829)
                     /locus_tag="VGA15_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9590588.1"
                     /translation="MRILSTLVAGLSVLLLPASVAAQEFPTKPIRLIVPFPAGGPNDT
                     IARVIGQRMSELIKQPVLIDNRGGQGGVLGTDAVAKAAPDGYTIAISSAGALTISPSM
                     ERVAYDTRRDFAPITLVATVPEMLVVATNVRANNMSELIALAKAQPGKLNFASSGAGS
                     LTHLAGELLKLKANIDIVHVPYRGAAPAVNDLLGQQVQMAFLDLPILSPQIKAGNLRP
                     IAIGSPERAPTAMDVPTTAEVGMPDLKIENWYGMVAPAGTPANIVATLNKIATEAILD
                     AAVKEKLAGQGARLIGGTPEHFGHFINAEIEKWAKVIKDAEVETAK"
     gene            complement(2040..3293)
                     /locus_tag="VGA15_22950"
     CDS             complement(2040..3293)
                     /locus_tag="VGA15_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9590589.1"
                     /translation="MLGLNLVQRQRFCRLLQSALRVTLIGSTIALTAGCATIAPRNVL
                     PQAIAGQTELAGFHNIRFWGDAPAREIQAIMMADAPKAEMQLSSAVARHPPIANLLAI
                     SGGAEDGAFGAGLLAGWSETGTRPTFDLVTGVSSGALIAPFAFLGREHDNQLREIFTK
                     YGRKDIFTYNVPGLLEGAALADDAPLARLIAKYVDDAFLQEIARERMKGRVLLIGTTN
                     LDTQRPVLWDMGRIAMSNNREAMALFRKILLASATLPGVFPPVRIQVRVGGQNYDELH
                     VDGGVTRQVFIAPSIFSFVSHDQRAGRSAATPRLFVIRNGKIDPEWQSVSENVLSITQ
                     RSISTLIKNQGIGDLYRIYSITKRDGIDFNLASIPADFTQTSDEPFDQKYMIELFTRG
                     YDLGSHNYSWVKAPPGLELAAEAHN"
     gene            complement(3602..>4321)
                     /gene="hisN"
                     /locus_tag="VGA15_22955"
     CDS             complement(3602..>4321)
                     /gene="hisN"
                     /locus_tag="VGA15_22955"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX9590590.1"
                     /translation="LPFFRTSLSIDNKSTSHDFDPVTEADRAAEAVMRRLIKANFPQH
                     GIVGEEFGSEREDAEYVWVLDPIDGTKSFIAGFPIWGTLIALLHQGTPVFGMMHQPFI
                     GERFSGDSGSAHYHGPSGERKLAVRRCGSLKEATAFTTSPLLMNTADRAAFGRIEEAV
                     RLTRYGGDCYSYCMLAAGHLDLVVETELKPYDIAALIPIITGAGGIVTTWDGEPAQSG
                     GRIIAAGDPRVHEAAIKLLNS"
BASE COUNT          803 a         1289 c         1338 g          891 t
ORIGIN      
        1 tctgttcagc gaggaccgct tgatgctggt caccgcgcgg cgcggcgatc ttgccggccg
       61 tcgtcaaatc gattttcagg aggtgaggga tcgcgacttc gtcggactga ccaattccac
      121 cgcgcttcag atccatatcg ccagacacgc cgcgcggctc ggcctgcgct tgcgatttcg
      181 ggcgcggttg cgggatttcg acgcgatctg ccggatggtg gcggcggagg tcggcatcgc
      241 ggtggtgccg gaagccgccg cgaagcgctg tgcacggtcg atgccgatcg cgatgatcag
      301 gatccgcgac ccctgggcca atcgcaaact gacgatctgc gcccgcagct tcaagtcgct
      361 gccgcgccct gccaagcaac tggtcgagca tctgcgtaag gcggcgcagt aggcatcgcg
      421 gaattcgatg tgctgagcag gcgaagggtg aatttggttg aatgccgagt tatcctttga
      481 gcacggtcgg ctctctcccc ccttgtgggc aggggaatcg catatgactg aattgagccg
      541 cgcgttgatg cggtcgtggt ctcgcacaag ctctcagcgg gtcgtccctg cgaacgcagg
      601 gacccatcac ccctggcgtc aaaagtgaaa aaaggcgtct gcgccagcgc caaaatgaga
      661 gtcctcgccg ccgcgatgag cgcaattgcg ctcacgctgg ggtccctgcg ttcgcaggga
      721 cgacccgttg aaatgtcacg cgaaacaaac aagattcgtg tgcggaccgc atagaaaatt
      781 ccatatgcga tagccctgcc cgcaagcggg gagggaacgc attcgcggag acgggcggct
      841 tatcgattga tggaaggtgc tcacttcgcg gtttctacct cagcgtcctt gatcactttc
      901 gcccatttct caatttcggc gttaataaag tggccaaaat gttccggggt gccgccgatc
      961 agccttgcgc cctgaccggc gagtttctcc ttgacggcgg catcgagtat cgcctcggtc
     1021 gcgatcttgt tcagggtggc gacgatgttc gccggcgttc cggcgggcgc caccatgccg
     1081 taccaatttt cgatcttgag gtccggcatg ccgacttcgg cggtggtcgg aacgtccatc
     1141 gctgtcggcg cgcgttccgg cgagccgatc gcgatcggcc tcaggtttcc ggccttgatc
     1201 tgcggcgaaa ggatcggcag atcgaggaat gccatctgta cttgctgacc cagcaggtcg
     1261 ttgacggcgg gagctgcacc gcgatacggc acgtgcacga tgtcgatatt cgccttgagc
     1321 ttgagcaact cgccggccaa atgggtcagg ctgccggcgc cggacgatgc aaaattgagt
     1381 tttccgggct gcgccttggc gagcgcgatc agctcgctca tgttgttggc acgaacgttg
     1441 gtcgcgacca ccagcatctc cggcacggtc gctaccagtg taatcggtgc aaaatccctg
     1501 cgggtatcgt aggcgaccct ttccatgctg gggctgatcg taagcgcgcc tgcgctcgag
     1561 atcgcgatgg tgtatccgtc cggtgccgcc ttcgccaccg catcggttcc gagcacgccg
     1621 ccctgtccgc cgcgattgtc gatcagcact ggttgcttga tcagttccga catgcgctga
     1681 ccgatcaccc gcgcgatggt gtcgttgggg ccgccggccg gaaacggcac gatcagccgg
     1741 atcggctttg tgggaaattc ctgcgccgca accgacgccg gcaacagcag cacggataag
     1801 ccagccacca gcgtcgacaa aattcgcatg aaagtctcct cccttccggc ctccaggccg
     1861 catgcgcgtg cccgcgctct tatccttgcc ttaaccctac gggatggaat gctccatgaa
     1921 aagttgagat gcatggcgcc tgcggtgaat tgagaagcgg cacgagcgcg gcgtcagcgg
     1981 ggaggcttcg gtttcgcaaa ccttgaatca cggctggcgc gacgccaaca ggcgaacgtt
     2041 cagttgtgcg cctcggctgc caattccagc cccggcgggg ccttcaccca ggagtagttg
     2101 tggcttccga gatcatagcc acgggtaaaa agctcgatca tatatttctg gtcaaacggt
     2161 tcatcgctgg tttgggtaaa gtcagcagga atgctcgcga ggttaaagtc aattccgtcg
     2221 cgcttggtga tggaatagat gcggtagagg tctccgatcc cctgattctt gataagggtg
     2281 gagatcgaac gctgcgtaat cgacaggaca ttctcgctga cggattgcca ttccggatcg
     2341 atcttgccgt ttctgataac gaacagacgc ggcgtcgcgg ccgatcttcc ggctctttga
     2401 tcgtgggaaa caaacgagaa tatcgatgga gcgataaaga cctgtcgcgt gacgccgcca
     2461 tcgacatgga gttcgtcgta attctgtccg cccacccgca cctgaattcg gaccggcggg
     2521 aaaaccccgg gaagtgtcgc cgaggcgagc aatatcttgc ggaacagggc catcgcttcg
     2581 cgattgttgc tcatcgcgat ccgccccatg tcccacaata ccggcctttg cgtatcaaga
     2641 ttggtggtac cgatcagcag gactcggcct ttcatacgct ctctggcgat ttcctggaga
     2701 aacgcatcgt cgacgtattt cgcaattagc ctggccaacg gcgcgtcgtc ggccaacgcg
     2761 gctccttcga gcaaaccggg tacgttgtag gtaaaaatgt cctttcttcc atatttggta
     2821 aaaatctcgc gcagttgatt gtcatgttcg cgcccgagga aagcgaacgg agcgatgagc
     2881 gcgccggagc tgacgccggt gaccagatcg aaggttggcc gcgtgccggt ttcgctccag
     2941 ccggccagca gcccggcgcc aaaggcaccg tcttcggcac caccggatat cgccagaaga
     3001 ttcgcgatcg gcggatgcct tgcaactgcc gacgacaatt gcatctccgc tttcggcgca
     3061 tctgccatca tgatcgcctg gatttcacgg gccggggcgt caccccaaaa gcgtatgtta
     3121 tgaaatcctg cgagctcggt ttggccggct atcgcttgtg gcaacacatt gcgcggggca
     3181 atcgttgcac atccggcggt caaagcgatt gtcgaaccga tcaaggtgac gcgaagtgcg
     3241 ctctgcagca ggcggcaaaa gcgctgtctc tgcacaaggt tcaaaccaag cacttccggt
     3301 cgcttccctt cgtcgtcagc cggaccagcc ctgatccctt cgggtgtcga aagggcgtcc
     3361 tcaacgtggc gatcacagtg acattttctg aacttttcgg catttccatt tggggcttcc
     3421 ctgcagccgt ctgactcgca cttcaggtgt gaagcggcgg cggatcagtg ttagatttgc
     3481 ggcattgtcg cggccgatta ttgcggcatt gttgcggccg actacgccgc ggccgagcgc
     3541 caggcggcga ggcatcctgc attgttatcg ccaatgggca ggagagcagt gacgatttgg
     3601 gctagctgtt cagcagcttg atggcggctt catgcacgcg cggatcgccg gcggcgatga
     3661 tgcggccgcc gctttgggcg ggctcgccgt cccaggtggt gacgatgccg ccggcgccgg
     3721 tgatgatcgg aatcagtgcc gcgatgtcgt agggtttcag ttcggtctcg accacgaggt
     3781 cgagatggcc ggcggccagc atgcaatagg aatagcagtc gccgccatac cgcgtcagcc
     3841 gcaccgcttc ctcgatgcgg ccgaaggcgg cgcggtcggc cgtgttcatc agcagtggac
     3901 tggtcgtgaa cgcggtcgct tctttcagcg agccgcagcg ccgcaccgcc agtttccgtt
     3961 cgcccgacgg cccatggtaa tgcgccgagc cggagtcgcc ggaaaagcgc tcgccgatga
     4021 agggctggtg catcatcccg aacaccggcg tgccctggtg caacagcgcg atcagcgtgc
     4081 cccagatcgg aaagcccgcg ataaaggatt tggtgccgtc gatcggatcc agcacccaga
     4141 catattcggc gtcttcgcgc tcgctgccga attcctcgcc gacgatgcca tgctggggga
     4201 aattggcctt gatcagccgc cgcatcaccg cttccgccgc gcggtcggct tcggtcaccg
     4261 gatcgaaatc gtggctggtg cttttattgt cgatactcag cgacgttcga aagaacggca
     4321 g
//
LOCUS       DASWGM010000838         5135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1786374,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000838 DASWGM010000000
VERSION     DASWGM010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786374"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VGA15_22960"
     CDS             complement(<1..180)
                     /locus_tag="VGA15_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX9590591.1"
                     /translation="MSIEPRAPPPQFAARLRHALGGRAGWRGFFVQILFVAALAWIGY
                     EIAANARANLETQRIT"
     gene            complement(204..1220)
                     /locus_tag="VGA15_22965"
     CDS             complement(204..1220)
                     /locus_tag="VGA15_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9590592.1"
                     /translation="MKRVSLAFTLALAAGLSTQAATAQTLKTVKDRGMLSCGVSQGLP
                     GFSSPDDKGNWTGLDVDICRAVAAAIFNDPTKIKFVPLSAKDRFTALQSGEIDVLSRN
                     TTWTLSRDTSLGANFTGVTYYDGQGFLVKKSLKVNSALELNSASVCVQTGTTTEQNLA
                     DYFKGNNMKYEVIAFGTADEAVKAYESGRCDVFTTDVSGLYAERLKLSNPADHAVLPE
                     VISKEPLGPMVRHGDDQWFDIVKWVLFAMIDAEELGVTQKNVDDMAKSDKPELKRAFG
                     TDGNLGEQLGLTKDWMSRIVKAVGNYGESFDRNVGTGSKLGIGRGLNQLWNKGGIQYA
                     PPIR"
     gene            1555..2748
                     /gene="metC"
                     /locus_tag="VGA15_22970"
     CDS             1555..2748
                     /gene="metC"
                     /locus_tag="VGA15_22970"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEX9590593.1"
                     /translation="MNSSSNDGRSSPHKAETTLVTAGRDTKAQKGFVNPPVVHGSTVL
                     YPTAEDLHAHRGEFQYGRRGTPTTKALQEALMALEGPQCAGVGIAPSGLSAITTTLLA
                     VLKAGDHLLVCDNAYRPTRNFCDGLLAGYGIETTYFDPLIGAGVGQLFKPNTKAILLE
                     APGSQSFEMPDVPAIAAVAHGRGALVIDDNTWATPLYHRSLEQGVDISMQAATKYIGG
                     HSDIMFGTISANARAWPQLAETIRLLGVCAGPDDVFLALRGLRTLAVRLAQHHRSALD
                     MARWLATRPEVVRVLHPALESDPGHAIWKRDFTGASGLFSIVLKPAPQNAVDALLDTV
                     KLFGMGYSWGGFESLVIPFDCAPYRTATKWAPGGPALRLHIGLENVDDLKADLERGFA
                     AFNAV"
     gene            complement(2836..4176)
                     /gene="mgtE"
                     /locus_tag="VGA15_22975"
     CDS             complement(2836..4176)
                     /gene="mgtE"
                     /locus_tag="VGA15_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX9590594.1"
                     /translation="MVNPAFLAASMIEERAPDIVEALNDMPPELAVAVLLHLPPDRAI
                     EVLDQPGLEREPELFTLMPRDAAAKLLAGVSADRVADIFRQVEEPHSSELLDLLDPDT
                     RVNIRRLLEYPKDTAGSIMTTEFASVPSTYTVQQTLDYLRHVESSRETVYAIYVLDPH
                     SKKLVKTITLRRLITGDPAAPVLSVARPGRLITATPLMDREDVARLISKYDLLAVPVV
                     DHGRVIGIVTVDDIIDAIVQETTEDVQKFGGMAAINEPYMEIGFWQMMKKRGGWLCAL
                     FLSEMLTASAMQGYEGELEKAIVLTLFIPLIMSSGGNSGSQATSLLIRALALRELDIK
                     DWWRVALREIPNGLALGTMLGLVGIVRITLWQYLGFYDYGEHWPLIALTVGAALIGIV
                     MFGSLTGSMLPFILQRIGFDPASASAPFVATLVDVTGLVIYFSVASLILRGTLL"
     gene            4384..4944
                     /gene="yfcF"
                     /locus_tag="VGA15_22980"
     CDS             4384..4944
                     /gene="yfcF"
                     /locus_tag="VGA15_22980"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF011693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HEX9590595.1"
                     /translation="MRFLVSTGLFRSASSRLPSPNKLRSTMAMLLYADKQFSSPYVMS
                     AFVALTEKQIPFEVSTLDLAIGANNDPEYARSSLKQRVPTLIDGDFALSESSAIAEYL
                     NDVYPDPPIFSTDPRKRARARQVQAWLRSDFMPIRGRNGNGLPFSSGFEENAPCPEGA
                     RVVRPAVPNRAAAAAAFPCGSSEINH"
BASE COUNT          967 a         1589 c         1585 g          994 t
ORIGIN      
        1 cgtgatccgc tgcgtttcca ggttggcgcg ggcgttggcc gcgatctcat agccaatcca
       61 ggccagcgcg gcgacgaaca ggatctgaac gaaaaatccc ctccagccgg cccggccgcc
      121 gagcgcatgc cgcagcctgg cggcaaactg cggcggtggc gcgcggggct cgatgctcat
      181 cgccgcagcc gcttggccag cgatcagcgg atcggcggcg cgtactggat tccacccttg
      241 ttccagagct ggttgagccc acggccaatc ccgagctttg agccggtgcc gacgttgcga
      301 tcgaaggatt cgccgtaatt gccgaccgcc ttcacgatcc gtgacatcca atccttggtg
      361 aggccgagct gttcgccgag attgccatcg gtgccgaacg cccgcttcag ttcgggcttg
      421 tccgatttag ccatgtcgtc gacgttcttc tgggtgacgc cgagttcttc cgcgtcgatc
      481 atcgcaaaca gcacccattt cacgatatcg aaccattggt cgtcgccatg acgcaccatc
      541 gggccgagcg gctctttcga gatcacctcc ggcagcacgg catgatcggc cggatttgaa
      601 agcttcaaac gctcggcata gagccctgac acgtccgtgg tgaagacatc gcaacgtccg
      661 gactcgtagg ccttgacggc ttcgtcggcg gtgccgaacg cgatcacctc atacttcatg
      721 ttgttgccct tgaagtagtc ggcgagattc tgctccgtcg tggtgccggt ctgcacgcaa
      781 accgacgcgc tgttcagttc cagcgcagag ttcaccttga gggatttttt caccagaaaa
      841 ccctggccgt cataataggt cacgccggtg aaattggcgc caagcgaggt gtcgcgggac
      901 aacgtccagg ttgtattgcg cgaaagtaca tcaatttcgc cggattgcag tgccgtgaag
      961 cgatccttgg ccgacaacgg cacgaacttg atcttggtcg gatcgttgaa gatggcggcc
     1021 gcgaccgcgc gacagatgtc gacgtcaagc ccggtccagt tgcccttgtc gtcgggggag
     1081 gaaaagcccg gcaaaccctg gctcacgccg caggataaca tgccgcggtc cttgaccgtc
     1141 ttcagcgttt gcgcggtggc ggcctgggtc gagaggccgg cggcaagagc aagggtaaaa
     1201 gccagagata cgcgtttcat gacacagcct ttcaagggtc tttccgggaa cgtctgctta
     1261 tcatcgcata cgccgatggg ccaaccttgg cgatccgtta ccgtaaacgc cgcctgacaa
     1321 gcagtttggc gcgcagtctg gtcaaaaacg gatcgaaggt cgcggcaggc ccctcaacgt
     1381 gctgcgactg gaatagctcc cacacatcac agaacgactg gcgggccggg tcaaggggtt
     1441 gacggccctc ttctgtgtcc tgttattaac caacccagcc cgaacccgcc gtccggtggg
     1501 ctttcagagc aatgtcgcgg ctgcggcaaa aagtctttcg ggggcggcgg acccatgaat
     1561 tcctcaagca atgacggacg gtcgagcccg cacaaggcag aaaccacgct ggtgacggcg
     1621 gggcgcgaca caaaggcgca aaaaggcttc gtcaatcccc cggtcgtcca cggctcgacc
     1681 gtgctttatc cgacggcgga agatctgcac gcccatcggg gcgagtttca gtatggccgc
     1741 cgcggaacgc cgaccaccaa ggcgctgcag gaagcgctga tggctctcga aggcccgcaa
     1801 tgcgcgggcg tcggcattgc gccctcgggg ctgtcggcca tcaccacgac gctgctcgcg
     1861 gtgctgaagg ccggcgatca tctcctggtc tgcgacaacg cttatcgccc gacacgaaat
     1921 ttctgcgacg gattgctcgc aggctacggc atagagacga cctatttcga tcccctgatc
     1981 ggcgccggtg tcggccaatt gttcaagccc aacaccaagg cgatactgct cgaggcaccc
     2041 ggttcgcaat ctttcgaaat gcctgacgtt cccgcgatcg ccgctgtcgc gcatggccgc
     2101 ggggcgctcg tcatcgacga caacacctgg gcgacgccgt tgtatcaccg ttcgctggag
     2161 caaggcgtcg acatcagcat gcaagcagcg accaaatata tcggcggaca ttccgacatc
     2221 atgttcggca cgatatcggc gaatgcgcgg gcctggccgc agcttgccga aacgatccgg
     2281 ctgctcggcg tctgcgccgg gccggacgac gtgtttctcg cgctgcgcgg tctgcgcacg
     2341 ctcgcggtgc ggctggcgca gcatcatcga tcggcgctcg acatggcgcg ctggctggcg
     2401 acgcggcccg aggtcgtcag ggtgctgcac cccgcgctcg aaagcgatcc cggccacgcg
     2461 atctggaaac gggattttac cggggcctcc ggcctgttca gtatcgtcct gaagcccgcg
     2521 ccgcaaaacg ccgtcgatgc cctgctcgac accgtcaagc tgtttggcat gggctattca
     2581 tggggcggtt tcgagagcct tgtgattccg tttgactgcg ccccctatcg tacggcgacc
     2641 aaatgggcgc cgggtggacc ggccctgcgg ctccacatcg ggctcgagaa cgtcgacgat
     2701 ctcaaggccg accttgagcg tggctttgcc gctttcaacg ccgtttagcg ccccgtaccc
     2761 atcgtccgag agccggtggc ggccgtttga tgctcgccga aaaacgagcc aagcgcatcg
     2821 gcgaacgaag gaccgctaca gcagggtgcc gcgcaggatc agtgaagcga cgctgaaata
     2881 gatcaccaaa ccggtcacgt ccacgagggt cgctacgaac ggggccgaag cgctggccgg
     2941 gtcgaagccg atccgctgca ggatgaacgg aagcatggat ccggtcagcg agccgaacat
     3001 gacgatgccg atcagggcgg cgccgaccgt cagcgctatc agcggccagt gctcgccgta
     3061 gtcgtagaag ccgagatatt gccagagcgt aatgcggacg atgccgacaa ggcccagcat
     3121 cgtcccaaga gccagtccat tcgggatttc ccgcagcgcc acgcgccacc agtccttgat
     3181 atcgagttca cgcaaagcca atgcgcgtat cagaagcgat gtggcctgcg atccggaatt
     3241 gcctcccgaa ctcatgatca gcgggatgaa cagcgtcagc acgatggcct tctcgagctc
     3301 gccttcgtag ccctgcatcg cggatgccgt cagcatttcg gacaggaaca acgcgcacaa
     3361 ccagccgccg cgcttcttca tcatctgcca gaagccgatt tccatgtacg gctcgttgat
     3421 ggcggccatg ccgccgaact tctgcacgtc ttcggtggtt tcctgcacga tggcgtcgat
     3481 gatgtcgtcg accgtgacga tccctatgac gcgaccgtgg tcgacgaccg gcaccgcgag
     3541 caggtcgtac ttcgagatca ggcgtgcgac atcttcccga tccatcaggg gagtggcggt
     3601 gatcaggcgg cctggacggg ctaccgagag caccggtgct gccggatctc ccgtgatcag
     3661 ccggcgcaga gtgatcgtct tgaccagctt cttgctgtgc ggatccagca cgtagatcgc
     3721 gtagacggtt tcccggctgc tttccacgtg acgaagatag tcgagcgtct gctgcaccgt
     3781 gtaggtcgag ggaacgctgg cgaattccgt cgtcatgatc gagccggcgg tgtccttcgg
     3841 atattccagc agacggcgga tgttgaccct ggtgtcgggg tccagcagat cgagcagctc
     3901 ggagctgtgc ggctcctcga cctgccggaa gatgtcggcg acgcggtcgg ccgacacgcc
     3961 ggccagcagc tttgcggccg catcccgcgg catcagcgtg aagagttctg gttcgcgttc
     4021 cagccctggc tggtcgagga cttcaatggc gcgatcgggc ggcagatgaa gcagaaccgc
     4081 gactgcgagt tccggaggca tgtcgttcag ggcttcgacg atgtcgggcg cgcgctcttc
     4141 gatcatgctg gcggcgagga acgccggatt gaccatgttg tcggccttcg ccgccggatc
     4201 atcctggacg tcaaggtcgg tcatgattca catctccgtg ctgacgacag acggctgata
     4261 agctttgggc cttgccctgt aaataaagga aatcgaagcc gaacggcagg ccccgttgga
     4321 tggtattggt ggggtaagtc cagtcaagaa tccgcttgcg atcccggctg acctcacccg
     4381 cggttgcgtt ttcttgtttc tacgggtcta ttcaggtctg cttcaagccg gttaccatct
     4441 cccaataaat tgcgcagcac gatggccatg cttctctacg cagacaaaca gttctcgtcc
     4501 ccctatgtca tgtcggcctt tgtggcgctg acggagaagc agattccgtt tgaggtcagc
     4561 acgctggatc tggcgattgg cgccaacaac gacccggaat atgcaaggtc atctctcaag
     4621 cagcgcgtgc cgacgctgat tgacggtgac ttcgcgcttt ccgagtcgtc ggcgatcgcg
     4681 gaatatctca acgacgtcta tcccgatccc ccgatatttt cgaccgaccc gcgaaagcgc
     4741 gcccgcgccc ggcaggttca agcctggctg cgaagcgatt tcatgccgat ccgagggcgc
     4801 aatgggaacg gccttccgtt cagcagtggg ttcgaagaga acgccccctg ccctgaaggc
     4861 gcgcgggtgg tgcgtccggc cgtgcccaac cgagcggcgg ctgccgccgc atttccgtgc
     4921 ggttccagcg aaataaatca ttgatccgct gtcgtttggc gatagcctga acgttcgact
     4981 caaatccgga agtagaaatt catgggaagt gtggttcttc tcgacctgat gggcggcgtg
     5041 gcgctgctgc tgtggggcct gcacatggtt cacagcggca tcctgcgcgc cttcggtccc
     5101 gatctccgtc tgcttctggc gaaagcgctg aacaa
//
LOCUS       DASWGM010000839         4456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2325970,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000839 DASWGM010000000
VERSION     DASWGM010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325970"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VGA15_22985"
     CDS             <1..1153
                     /locus_tag="VGA15_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590596.1"
                     /translation="APDHSRDSREPPFGFWRRSYAMLVKEFIQLRRDRVSFAMIVMIP
                     VMQLMLFGYAINTTPRNLPTAVLLQEDSDLARSILKALENTAYFRFTHEVHDVAEFDN
                     LLLSGKVLFGVEIPRGFERAVRRGDHPALLVAADATDPVAAGSALGALGVVVQTALEH
                     DLFTGDPPSMPFEIRAHARYNPAGSSRLNIVPGLVGTILTMTMLIFTALSVTREIERG
                     TMESLLSMPIKPVEIMLGKILPYVLVGFIQATLIVGIGVLLFGVPVQGSLIMLALLST
                     LFITTNLSIGYTFSTIVQNQLQAMQLSMMFFLPSILLSGFMFPFAGMPVWAQYFGEGL
                     PLTHYLRIVRAIMLKGATLPNLQYDMIALLALMLLAMTIAVTRFRRTLD"
     gene            1181..2497
                     /locus_tag="VGA15_22990"
     CDS             1181..2497
                     /locus_tag="VGA15_22990"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9590597.1"
                     /translation="MPGQWWGSRGERRPAVPADFAHALTREILLTERLRVKVILVTLS
                     LLLAILTAAYAISPSSIERIWHGHLPVQPLYGAFLFFAVFELSVLRLLNLRLAQGRDV
                     PYARRYLGALIETSLPTAALYLQMSAMGPTQALAFVAPLVYFIFIILSTLRLDFWLST
                     FTGFVAAAELFAMAMFYHPAQFADDPAPEVGFHFVRSLVILVCGGLAGAVGMQLRRQF
                     EASILAATARDRVTNLFGQHVSPQVVERLLRAGAGAASETREVAVMFVDFRNFTAAAR
                     SRTPQKVVARLDDAFAVLVEILDRHGGIVNKFLGDGFLALFGAPIADPDAAARAVAAA
                     QEMLAAMAQHNDGHEWKLRIGIGIHIGAVVAGNVGSPRRKEYTVMGDTVNFAARLESL
                     NKDFNSQLLVSAAIRDAIGETGSNAVPLGEVAIRGYDRPMTVWQLG"
     gene            2572..3483
                     /locus_tag="VGA15_22995"
     CDS             2572..3483
                     /locus_tag="VGA15_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX9590598.1"
                     /translation="MQRRYITVDVFTDRAFGGNPLAVVLDAGGLATAQMQSIASEFNY
                     SETTFVLPPQDPAHDAQVRIFTVNREIPFAGHPNVGTAFVLATQASKPPARLLFEENA
                     GLVPVEILAEEGSVVGAELTAPQALKKLTPFSAEQAAACISLAAADIRTDRHTPQIVS
                     VGLPFLVVELTSRDALRRARPDASAFARTFPCDGSDALYFYTRDVPPAEACDLQARMF
                     HPGSSGLSEDPATGSATVAAAALLADLASQRDGELKLRIGQGVDMNRPSLLLTRVVKQ
                     NGVVASAHVGGACVQMMEGTFPLAGEG"
     gene            complement(3697..4155)
                     /locus_tag="VGA15_23000"
     CDS             complement(3697..4155)
                     /locus_tag="VGA15_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX9590599.1"
                     /translation="MARKKLFTIGYEQTPAKAVLDELEQAGVKLLVDVRAVAASRRPG
                     FSKNQLASGLDERGISYLHLRGLGTPKNGRDAARGGKFDALHKIYAKHLKTPQAKEEL
                     DELSALVKKSGPVCILCYERDHLHCHRQWIAEIIEDRDGVRIENLVAPQV"
     gene            4274..>4456
                     /locus_tag="VGA15_23005"
     CDS             4274..>4456
                     /locus_tag="VGA15_23005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9590600.1"
                     /translation="MSHSRLGAAAQSEPDGYTLLIGGLSNMAFNSALYSKLSYDPQRD
                     FVPVAVVYKFGYVMVGR"
BASE COUNT          711 a         1393 c         1437 g          915 t
ORIGIN      
        1 cgcacccgac catagccgcg acagcaggga gccgccattc ggcttctggc ggcgcagcta
       61 tgcgatgctg gtcaaggaat tcatccagct gcggcgcgac cgggtatcgt tcgcgatgat
      121 cgtgatgatc ccggtgatgc agctgatgct gttcggctac gccatcaaca ccacgccgcg
      181 caacctgccc acggccgtgc tgctgcagga ggactctgac ctcgcgcgct cgatcctcaa
      241 ggcgctggag aataccgcct atttccgctt cacccatgaa gtgcacgacg tcgcggagtt
      301 cgacaatctg ctgctctccg gcaaggtgct gttcggcgtc gaaatcccgc gcggttttga
      361 gcgggcggtg cggcgcggcg atcatccggc gctgttggtc gcggcggacg cgaccgatcc
      421 ggtggcggca ggctccgcgc tgggtgcact cggcgtcgtg gtgcagaccg cgctggaaca
      481 tgacttgttt acgggcgatc cgccgtcgat gccgttcgag atccgcgccc atgcgcgcta
      541 caacccggcg gggtcgtcgc ggctgaacat cgtgcccggc ctggtcggca ccatcctgac
      601 catgaccatg ctgatcttca ccgccctttc ggtgacccgc gagatcgagc gcggcaccat
      661 ggagagcctg ctgtcgatgc cgatcaagcc ggtcgagatc atgctcggca agatccttcc
      721 ttacgtgctg gtcggcttca tccaggccac gctgatcgtc ggcatcggcg tgctgctgtt
      781 cggcgtgccg gtgcagggca gcctgatcat gctggcgctg ctctcgacgc tgttcatcac
      841 caccaatctg tcgatcggct atacgttctc gaccatcgtg cagaaccagc tgcaggcgat
      901 gcagctgtcg atgatgttct tcctgccgag cattcttttg tccggcttca tgtttccgtt
      961 cgcgggcatg ccggtctggg cgcaatattt cggcgaaggc ctgccgctga cgcattacct
     1021 gcggatcgtc cgcgccatca tgctgaaggg cgcgaccctg ccaaatctgc aatacgatat
     1081 gattgccctg ctggcattga tgctgctggc gatgacgatc gcggtgacgc gcttccgccg
     1141 caccctcgat tgatgcagaa tgggcttcgg agcataggcg atgccggggc aatggtgggg
     1201 cagtagaggt gagcggcggc cggcggtgcc ggccgatttc gcgcacgcgc tgacccgcga
     1261 aatcctgttg accgagcggt tgcgggtcaa ggtgatactt gtcaccctct cgctgctgct
     1321 ggcgattttg accgcggcct acgcgatctc gccctcaagt atcgagcgca tctggcatgg
     1381 ccatctcccg gtccagcctc tgtatggggc ctttcttttc ttcgccgtgt tcgaactgtc
     1441 ggtgcttcgg ctgctcaatc tgcggctggc gcagggccgc gacgtgccgt atgcgcgtcg
     1501 ctacctcggt gcgttgatcg aaaccagcct gcccaccgct gcgctgtatc tgcaaatgtc
     1561 ggcgatgggg ccgacgcagg ccttggcctt cgtggcgccg ctggtctatt tcattttcat
     1621 catcctttcc acgctgcggc ttgatttctg gctctctacc tttaccggct tcgtcgcagc
     1681 ggccgagctg ttcgccatgg cgatgttcta tcatccggcg caattcgctg acgacccggc
     1741 gcctgaggtc ggatttcact tcgtccgcag cctcgtcatt ctggtttgcg gcgggcttgc
     1801 cggggccgtg gggatgcaac tgcgccgcca gttcgaggcc agcattctgg ctgcgacggc
     1861 gcgggaccgc gttacaaacc tgttcggcca gcacgtctcg ccgcaggtgg tggaacggct
     1921 gttgcgcgcg ggcgcgggcg ccgccagcga gacccgcgag gttgccgtga tgttcgtcga
     1981 tttccgcaac ttcaccgcgg cggcgcgttc acggacgcca cagaaggtcg tggcgcggct
     2041 ggatgacgcc tttgctgtgc tggtcgagat tctcgatcgc catggcggca tcgtgaacaa
     2101 gtttctcggc gacggctttc tcgcgctgtt cggcgcgccg attgcggatc ccgatgcggc
     2161 ggcgcgcgcg gtcgcggccg cgcaggaaat gctggccgcg atggcgcagc acaatgacgg
     2221 gcacgaatgg aaactgcgga tcggcatcgg tatccacatc ggtgccgtag tggccggcaa
     2281 tgttggctcg ccccgccgca aggaatatac cgtgatgggc gacaccgtga actttgccgc
     2341 ccggcttgaa tcactcaaca aggatttcaa ttcgcagctc ttggtgtcgg ccgctattcg
     2401 cgacgcgatc ggggagaccg gcagcaatgc agtgccgctc ggggaggtcg cgatcagggg
     2461 ttatgaccgg ccgatgacgg tgtggcagct cggataaaaa ggctggctct cgctgggctt
     2521 gacgaaccga tccgcgtcat gtttcacccg atgcaaaatc ggagacctca gatgcagcgg
     2581 cgctatatca ccgtggacgt tttcaccgac cgcgccttcg gcggcaatcc gctggcggtg
     2641 gtgctggatg ccggcgggct cgcgaccgcg cagatgcaat caatcgcatc cgagttcaat
     2701 tattccgaaa cgacctttgt gctgccgccg caggacccgg cgcacgacgc gcaggttcgg
     2761 atttttaccg tgaaccgaga gatccccttc gcgggtcatc ccaacgtcgg taccgccttt
     2821 gtgctggcaa cgcaggcgtc aaaaccgccg gcgcggctgc tgtttgagga aaatgccggc
     2881 ctcgtgccgg tcgaaatcct cgcagaagag ggcagcgtcg tcggcgccga actcaccgcg
     2941 ccgcaggccc tgaagaagct cacgccgttc agcgccgagc aggccgcggc ctgtatctcg
     3001 ctggcggctg cggatatcag gaccgaccgg catacaccgc aaatcgtctc ggtggggctg
     3061 ccgtttctgg tggtggagct gacctcccgc gacgcgctgc ggcgggccag gcccgacgca
     3121 tcggcattcg cccgtacatt tccatgcgac ggcagcgatg cgctttactt ctacacccgc
     3181 gacgtgcctc cggccgaggc gtgcgatttg caggcgcgga tgtttcatcc gggatcgagc
     3241 gggttgtccg aagatcccgc caccggcagc gccaccgtcg cagccgcagc actgctcgcc
     3301 gatcttgcaa gccaacgcga tggcgagctg aagctgcgca tcgggcaggg cgtcgacatg
     3361 aacaggccaa gcctgctgct gacgcgtgtt gtgaagcaga acggcgtggt cgcgtccgcg
     3421 catgtcggcg gggcttgcgt gcagatgatg gaggggacgt ttccgttggc gggcgagggg
     3481 tgaggcggag cgttttcaaa ctggacacgt cattccggga tgcgccttcg gtcggcaatt
     3541 gccgacctga ggcgacaggc ccggaatcca taatccccgc ggtggttatg gatcccgggc
     3601 tcgcgcggag cctgtcatcg ggccgcgctt tgcgcggaca cgttggcgcg ccccggaatg
     3661 acggccttta ttggtgtcgt gtgcatcaaa atgactctac acctgcggcg cgaccagatt
     3721 ttcgatcctg acgccgtcgc ggtcttcgat gatctcggcg atccactgcc gatggcagtg
     3781 cagatgatcg cgctcatagc agaggatgca gaccgggccg gatttcttca ccagcgccga
     3841 caattcgtcg agttcttcct tggcctgcgg cgtcttcaaa tgcttcgcgt agatcttgtg
     3901 cagcgcatcg aacttgccgc cgcgcgcggc gtcgcggccg ttcttcgggg tgccgagccc
     3961 gcgcagatgc aggtatgaaa tgccgcgctc atcgagcccg gacgcgagct ggttcttcga
     4021 aaagccgggg cggcgagaag ctgcgaccgc gcgcacgtcg accagcagct tcacgccggc
     4081 ctgctcgagt tcgtcgagca ccgccttggc gggcgtctgt tcatagccga tggtgaagag
     4141 ctttttgcgt gccatgtcgg gtccaggcat tgccgatgtc atcggctcaa ggctacgtca
     4201 gtcctgaaat gaattccaca accgctcagg ttatgttggg ccaggcgcgg tctccattgc
     4261 cgccatctgc gccatgtcgc atagtcggct cggagccgca gcccagagcg agccggatgg
     4321 ctatacgcta ttgatcggcg ggctctccaa catggccttc aattcagcac tgtattcaaa
     4381 gctttcttac gatccgcaga gggatttcgt gccggtcgcc gtggtctaca agttcggcta
     4441 tgtgatggtc ggccgc
//
LOCUS       DASWGM010000840         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_108537,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000840 DASWGM010000000
VERSION     DASWGM010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108537"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VGA15_23010"
     CDS             <1..1099
                     /locus_tag="VGA15_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9590601.1"
                     /translation="LLAIILGIGAAILLVRDVSTGIRSIVAPMQALGSGDLTAQVPHQ
                     GETTEIGSMADTLQVFKDALVAKKASDEAAAIDAGAKIRRGQRVDNITREFETLIGEL
                     VGSLSTASTQLEASAGNLTSTAERAQKLTTVVAAASEEASTNVRSVASATEEMASSIN
                     EIGRQVQESARMATDAVDQARKTNDRVSELSKAAARIGDVVELINTIAGQTNLLALNA
                     TIEAARAGDAGRGFAVVASEVKALAEQTAKATGEIGLQITGIQAATEESVNAIKEISG
                     TIEKLSEISSTIASAVEEQGAATQEISRNVLQAAHGTQQVSSNVTDVQRGAGETGAAS
                     SQVLSAAKSLSGDSNRLKLEVGKFLNAVRAA"
     gene            1394..>1714
                     /locus_tag="VGA15_23015"
     CDS             1394..>1714
                     /locus_tag="VGA15_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9590602.1"
                     /translation="MSSDSPILAGTSRTPRRGIAFGLAPRIMALSVSGIVLLGVCVTG
                     VAWYLLERAAIKAATERVETNMRVAWDVLRAHGKSFSMVDGKLLASDHVLNGDAELVD
                     KVKSL"
BASE COUNT          342 a          533 c          546 g          293 t
ORIGIN      
        1 gctactggca attatcctgg gaataggcgc tgcgatcctg ctggttcgcg acgtctccac
       61 tggaatcagg tccattgttg cgccgatgca ggcgctgggc agcggcgacc tgaccgcgca
      121 ggtgccgcat cagggtgaga cgaccgagat cggctccatg gcggacacgt tgcaggtgtt
      181 caaggacgcg ctggtcgcca aaaaggcctc cgacgaggct gcggcgattg acgccggcgc
      241 taaaatccgg cggggccagc gtgtcgacaa cattacgcgg gaattcgaaa cgctgatcgg
      301 cgaactggtc ggctcactgt ctacggcctc gacccaactt gaggcctccg ccggcaatct
      361 cacctcgacc gcggagcgcg cgcaaaaact caccaccgtg gttgcggcgg cgtccgagga
      421 ggcctccacc aacgtacggt cggtggcctc cgcaaccgag gagatggcct cgtcgatcaa
      481 cgagatcggc cgtcaggtcc aggaatcggc gcggatggcc accgacgccg tcgatcaggc
      541 gcgcaagacc aatgaccgcg tcagcgaatt gtcgaaggcg gcagcccgca tcggcgacgt
      601 cgtcgaactc atcaatacca tcgccggaca gaccaatcta ttggccctga acgccaccat
      661 cgaagcggca cgggccggcg atgccggtcg cggcttcgcg gtcgtcgcat ccgaggtcaa
      721 ggcgctggcc gagcagaccg cgaaagccac cggcgagatc ggtctgcaga tcaccggcat
      781 ccaggcggcg acggaggagt cggtcaacgc tatcaaggaa atcagcggca ccattgaaaa
      841 attgtccgag atttcttcga cgatcgcttc cgcggtggaa gagcaggggg ctgcgacgca
      901 ggaaatctcc cgcaacgtgc tgcaggcggc tcacggcacc cagcaggtct catccaacgt
      961 caccgacgtg caacgcggcg ccggcgaaac cggagcggct tcgtcgcagg ttctctccgc
     1021 ggcaaaatcg ctgtccggcg acagtaatcg cctcaagctc gaggttggca aattccttaa
     1081 cgcggtaagg gccgcctgac gacgaggagc ccggcccaag cgcggccgga gctccaccct
     1141 tcgatcatga ggtttttgaa agctcgtgcc cgataaagcg gcgacgatgg acgatccgtc
     1201 acgtgcgccg cctcaccatg aagagcctgc aaaacctcgt aactctactg acaggcaatt
     1261 aacacgaatc gagcgcgcgc gacttatgat tccgcgcagc ggcgccactt cgcacaaggt
     1321 cgagtggcga tcgccgatgc attcgcggtg ctctttgggc cgaatggcat catgctctct
     1381 tggagattcg aaaatgtcgt cggattcgcc gattctcgcc gggacgtccc ggacccctcg
     1441 tcgcggtatc gctttcgggc tcgcgccacg gatcatggcc ctgtcggttt ccggcatcgt
     1501 tctgttgggc gtctgcgtga cgggcgtcgc ctggtatctt ctggaacgcg cggcgatcaa
     1561 agcggccacc gagcgggtgg aaaccaacat gcgggttgcg tgggacgttc tccgcgctca
     1621 cggcaagagt ttcagcatgg tggacggcaa gctgctggcg agcgatcatg tcttgaacgg
     1681 tgacgcggaa ttggtcgaca aggtcaagag cctc
//
LOCUS       DASWGM010000841         5224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1321956,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000841 DASWGM010000000
VERSION     DASWGM010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321956"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1177
                     /gene="pstC"
                     /locus_tag="VGA15_23020"
     CDS             188..1177
                     /gene="pstC"
                     /locus_tag="VGA15_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX9590603.1"
                     /translation="MAVQSDVMEAAGPYDRAKALNAFKFGDVTFYWITRICAISVLLI
                     LGGIILSLIVGAWPAMKQYGFAFLWTQRWAPSADPPVLGALGPMYGTLVTSFIAMLIA
                     IPVGLGIAIFLTELCPQWLRRPIGIAIELLAGIPSIIYGMWGFFVLGPFLAYTFQPFM
                     IGLFGGVPVLGSIFGGPPSYLSLFNAALILAIMVLPFITAISVDVFKTVPPVLKEAAY
                     GVGCTTWEVVRNVVIPYTRVGVIGGIMLALGRALGETMAVTFIIGNSFRISSSIFAPG
                     TTISAAIASEFAESDGLHQSGLILLGLLLFVLTFFVLAAARLMLLRLEQKAGN"
     gene            1183..2028
                     /gene="pstA"
                     /locus_tag="VGA15_23025"
     CDS             1183..2028
                     /gene="pstA"
                     /locus_tag="VGA15_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX9590604.1"
                     /translation="MNPIYTRRRRVDLIIRGLCFAAALFGVTWLALILFTLLFNGVAG
                     LNVQIFTQNTPPPGSNEGGLLNAITGSVIMTVIGVGIGAPLGLFAGTYLAEYGRNDRL
                     TSVIRFINDILLSAPSIIIGLFIYGAIVVPMGGFSAFAGCLALAVIVIPVVVRTTEDM
                     LLLVPNPLREAASALGLPRSLVIKRIAYRAARSGLITGVLLATARVAGETAPLLFTAL
                     SNQFFSLDLTKTMANLPVTINNFVQSPYVYWKQLAWSGALIITLTVLALNIGARILGA
                     ERTAK"
     gene            2025..2849
                     /gene="pstB"
                     /locus_tag="VGA15_23030"
     CDS             2025..2849
                     /gene="pstB"
                     /locus_tag="VGA15_23030"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX9590605.1"
                     /translation="MNDLSVSVSVPNAPVPSMAGHAEVPAKVTARNLNFYYGEHPALK
                     NINLTLGTHRVTAFIGPSGCGKSTLLRIFNRMYDLYPGQRATGQLMLDQTNILDPKLD
                     LNLLRARVGMVFQKPTPFPMTIYENIAFGIRLYEKIPRSEMDGRVEKALRGGALWNEV
                     KDKLNASGLSLSGGQQQRLCIARTVAVRPEVILFDEPCSALDPISTAKIEELIQELAE
                     DYTIAIVTHNMQQAARVSDKTAFMYLGELIEFDETNKIFTSPSDRRTQDYITGRFG"
     gene            2860..3576
                     /gene="phoU"
                     /locus_tag="VGA15_23035"
     CDS             2860..3576
                     /gene="phoU"
                     /locus_tag="VGA15_23035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX9590606.1"
                     /translation="MASEHTAKAFDSDLQELTRLVAEMGGLAERMIVDSVDALVRRDI
                     ALGKRVVANDAEIDNLQRVIEERAVLTIARRQPMAVDLREIVGAMRVATDLERIGDLA
                     KNMGKRVAALENDFHPLKLIRGLEHMTDLVQSQVKSVLDAYAAHDLPAAMSVWKGDEE
                     VDAICTSLFRELLTYMMEDPRNISFCIHLMFCAKNIERIGDHATNIAETVFYMIEGQQ
                     ILDKRPKGDMTTFATTVPGN"
     gene            3674..4375
                     /gene="phoB"
                     /locus_tag="VGA15_23040"
     CDS             3674..4375
                     /gene="phoB"
                     /locus_tag="VGA15_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX9590607.1"
                     /translation="MSARILVVEDEEALTTLLRYNLDAEGYDVETVGRGDDADTRLKE
                     RVPDLIVLDWMLPGLSGIELCRRLRARPETRSLPIIMLTARGEESERVRGLATGADDY
                     IVKPFSVPELLARVKGLLRRASPERLATVLTYGDIELDREKRRVARSGRPIDLGPTEY
                     RLLEFFLEHPGRVFSREQLLDSVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTV
                     RGAGYALDDRFAKVE"
     gene            complement(4573..5082)
                     /locus_tag="VGA15_23045"
     CDS             complement(4573..5082)
                     /locus_tag="VGA15_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX9590608.1"
                     /translation="MTVLTWTDDRVEQLKKLWESGLSASQIAAELGNVTRNAVIGKVH
                     RLGLSGRAKSPSSAAPRQRKARPAQHMMRVSRPVSRGNTALAHAFEVEMEPDPIAFDN
                     VVPMSQRLSLLELSEATCHWPIGDPSSSEFFFCGGKALSSLPYCAHHSRIAYQPASDR
                     RRQPPKPTR"
BASE COUNT          959 a         1639 c         1613 g         1013 t
ORIGIN      
        1 caagggcgac aagatggccg aggaactcga ctacattccg atgccggagc cggtggtcaa
       61 gctgatcgaa aagacctggt cggctgatat caagagctga gtcgaaaagt ttcgttcccg
      121 gacgcggtgc agcgtccggg aacgagaccc atcaagtaaa acagtatagg ggattggcgt
      181 ggcagacatg gccgttcaga gcgatgtaat ggaagccgcc ggaccttacg accgcgccaa
      241 ggcgctcaac gctttcaaat tcggcgacgt tacattctac tggatcaccc gcatttgcgc
      301 gatttcggtg ctgctcatcc tcggcggcat cattctctcg ttgatcgtcg gcgcatggcc
      361 ggcgatgaag caatacggct tcgcgttcct gtggacgcag cgctgggcgc cgtcggccga
      421 tcccccggtg ctcggggcgc tcggtcccat gtacggcacg ctggtgacct ccttcatcgc
      481 gatgctgatc gcgataccgg tcgggctggg tattgcgatc tttctgaccg agctttgtcc
      541 gcaatggctg cgccgtccga tcgggatcgc catcgaattg ctcgcgggca ttccgtcgat
      601 catctacggc atgtgggggt tcttcgtgct ggggccgttc ctggcctata cgtttcagcc
      661 gttcatgatc ggcctgttcg gcggcgtccc cgttctcggt tcgattttcg gaggcccgcc
      721 gtcctacctc agcctgttca acgcggcgct gatcctcgcg atcatggtgc tgccgttcat
      781 cacagcgatc tcggtcgacg tgttcaagac ggtgccaccg gtgctcaagg aggcagccta
      841 cggcgtcggc tgcaccacat gggaagtcgt ccgcaatgtg gtgatcccct acacccgcgt
      901 cggcgtcatc ggcggcatca tgctggcgct cggccgcgcg ctcggcgaaa ccatggcggt
      961 caccttcatc atcggcaatt cgttccgcat ctcgtcttcg atcttcgcgc cgggcaccac
     1021 gatatcggcg gcgatcgcat ccgagtttgc cgaaagtgac ggtttgcacc agtccggcct
     1081 gatcctgctc ggcctgttgc tgttcgtgct gaccttcttc gttctggccg cggcgcgctt
     1141 gatgctgctg cggctggaac agaaagccgg gaactgaaga tcatgaaccc gatttacacg
     1201 cgccgccgcc gcgtcgacct catcatccgc gggctttgct ttgcagccgc cctgttcggc
     1261 gtgacctggc tggcgctgat cctgttcacc ctgctgttca acggggtcgc gggactgaac
     1321 gttcagatct tcacccagaa caccccgccg ccgggatcga acgaaggcgg cttgctgaac
     1381 gcgatcaccg gttcggtcat catgaccgtg atcggcgtcg gcatcggcgc gccgctcggt
     1441 ctgttcgccg gcacctatct cgccgaatac ggcaggaacg accggctcac ctcggtgatc
     1501 cgcttcatca acgatatcct gttgagcgca ccctcgatca tcatcggtct gttcatctac
     1561 ggcgccatcg tggtgccgat gggcgggttc tcggcttttg ccggatgtct ggcgctcgcc
     1621 gtgatcgtga ttcccgtggt ggtccgcacc accgaagaca tgctgctgct ggtgcccaat
     1681 ccgctgcgcg aggcggcctc cgcgctcggc ctgccgcgct cgctcgtcat caagcgaatt
     1741 gcctaccgcg cggcgcgctc cggcctgatc accggggtgc tgctggcgac cgcgcgcgtg
     1801 gctggcgaga ccgcgccgct gttgtttacc gccctcagca atcagttctt cagcctcgac
     1861 ctcaccaaga cgatggccaa cctgccggtc accatcaaca acttcgtgca gagcccctac
     1921 gtctactgga agcagctcgc ctggagcgga gccctgatca tcactctcac cgtactcgcc
     1981 ctgaatattg gcgcgcgcat tcttggcgcc gagaggactg caaaatgaac gatctttctg
     2041 tatccgtgag tgttccgaac gccccggtgc cgtcaatggc gggccacgca gaggtgccgg
     2101 caaaggttac ggcccgtaat ctgaatttct attacggcga gcaccctgcg ctgaagaaca
     2161 tcaacctcac gctgggcacc catcgcgtca ccgccttcat cgggccgtcc ggttgcggca
     2221 aatcgacatt gctgcggatt ttcaaccgga tgtacgatct ctatccgggc cagcgcgcca
     2281 ccggtcagct gatgctcgac cagaccaaca tcctcgatcc caagcttgat ctcaatctgt
     2341 tgcgcgcgcg cgtcggcatg gtgttccaga agccgacgcc gtttccgatg acgatctatg
     2401 agaacatcgc gttcggcatc cggctttacg aaaagattcc cagatccgag atggacggcc
     2461 gcgtcgagaa ggcgctgcgc ggcggcgcgc tgtggaacga ggtcaaggac aagctgaacg
     2521 cctctggcct cagcctgtcc ggcggccagc agcagcggct ctgcatcgcg cgcaccgtgg
     2581 cggtgcggcc cgaggtgatc ctgttcgacg agccgtgctc ggcgctcgat ccgatctcga
     2641 ccgccaagat cgaggaattg atccaggaac tggcggagga ctacaccatc gccatcgtca
     2701 cccataacat gcagcaggcc gcgcgcgtct ccgacaagac cgccttcatg tatctcggtg
     2761 aactgatcga attcgacgag accaacaaga tattcacctc gccgagcgat cgacgcaccc
     2821 aggactacat caccggccgg ttcggctgag gagagaaaca tggcttctga acataccgcc
     2881 aaggcgttcg acagcgatct gcaggaattg acccggttgg tcgcggagat gggcggactt
     2941 gccgaacgca tgatcgtcga ttccgtggac gcgctggtcc gccgcgatat cgcactcggc
     3001 aagcgtgtcg ttgccaacga cgccgaaatc gacaatttgc agcgcgtgat cgaggaacgc
     3061 gcggtgttga ccatcgcgcg gcgccagccg atggcggtcg acctgcgcga aatcgtcggc
     3121 gccatgcggg tcgccaccga tctcgagcgg atcggcgatc ttgccaagaa catgggcaag
     3181 cgggtcgcgg ccctggaaaa cgatttccat ccgctgaagc tgatccgcgg cctcgagcac
     3241 atgaccgacc tcgtgcagtc gcaggtcaaa tcggtgctcg acgcctacgc ggcgcacgat
     3301 ttgccggcgg cgatgtcggt ctggaagggc gacgaggaag tcgacgccat ctgtacctcg
     3361 ctgttccgcg aactcctgac ctatatgatg gaagatccgc gcaacatcag tttctgcatc
     3421 catctgatgt tttgcgccaa gaacatcgag cggatcggcg atcacgccac caatatcgcg
     3481 gaaaccgtgt tctatatgat cgaaggccag cagatactcg acaagcgccc gaaaggcgat
     3541 atgaccacct ttgccacgac cgtacccggg aattgagaaa gcgttttcga gcgaaagcct
     3601 gcccggcacg tgatgcaggg tggagagcca tttgcgtgaa gaaaatccgt tcaaacaaag
     3661 agagagctac gccatgagcg cgcgcattct ggtggtggaa gacgaagaag cgctcaccac
     3721 gctgttgcgc tacaacctcg acgccgaagg ctacgatgtt gaaacggtcg ggcgcggcga
     3781 cgacgccgac acccgtctga aggagcgcgt tcccgacctg atcgtgctcg actggatgct
     3841 gccgggcctg tccgggatcg agctgtgccg ccgcttgcgg gcacggccgg agacgcggtc
     3901 gctgccgatc atcatgctga ccgcgcgcgg cgaggaaagc gaacgggtca gggggctggc
     3961 gaccggcgcc gacgattaca tcgtcaagcc gttctcggtg ccggaattgc tggcgcgggt
     4021 gaagggtctg ttgcggcgcg ccagtcccga gcgcctggcc accgttctta cctatggcga
     4081 catcgagctc gaccgcgaaa agcgccgggt cgcccgctca ggccgcccga tcgacctcgg
     4141 cccgaccgag tatcgcctgc tggaattttt tctggagcat ccgggacggg tgttcagccg
     4201 cgagcagttg ctcgacagcg tgtggggccg cgatatctat atcgacgagc gtacggtgga
     4261 tgtgcatatc gggcggttgc gcaaattgct caaccccggc cgcgagcagg atccgatccg
     4321 caccgtgcgc ggcgccggct acgcgctgga tgatcggttt gcgaaagtgg aataggatgg
     4381 cggcaaaata atatccgccg tcgtcccggc gaacgccggg acccatacgc cgtgacgtat
     4441 cgatcttgcg aaaacgtgga gcaactacct gacgtaacca cacaaagctg gtggttatgg
     4501 gtcccggcgt tcgccgggac gacgaagtaa taatcgcgtt tagagatcgg acctcggcac
     4561 gaagtccgta atctaccgcg tcggcttcgg cggctggcgg cggcgatcgc tcgccggctg
     4621 ataggcgatc cgcgagtgat gtgcgcaata gggcaggctg cttagcgcct tgccgccgca
     4681 gaagaagaat tccgaactgg agggatcgcc gatcggccag tggcaggtcg cctcactcag
     4741 ttccagcagc gacagccgct ggctcatcgg caccacattg tcgaaggcga tcgggtccgg
     4801 ttccatctcg acttcgaaag cgtgggccag cgcggtgttg ccgcgcgaga ccggacgcga
     4861 aacccgcatc atgtgctggg ccgggcgggc cttgcgctga cggggggccg ccgaggaggg
     4921 gctcttggcg cggcccgaaa ggccgagccg gtggactttg ccgattacgg cattacgcgt
     4981 cacatttccg agttccgcgg cgatctggct ggccgaaagt ccgctctccc agagtttttt
     5041 cagttgctcg acgcgatcgt cggtccaggt caataccgtc atcgcaattt tcccttcgtg
     5101 tcgcgccggc cattctcgag gcggcggtgc gactccattc gttcaaaaac cctgcaggca
     5161 gaatccctta gccctcgccg ggcgcggaaa gcgcgcccgg gcgtttacgc cgaacactgg
     5221 acga
//
LOCUS       DASWGM010000842         5536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2326409,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000842 DASWGM010000000
VERSION     DASWGM010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326409"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VGA15_23050"
     CDS             complement(<1..457)
                     /locus_tag="VGA15_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590609.1"
                     /translation="MSSDGMRSARSFAIILIVHLAVIVLWQVLVDAFHVPKFVLPSPL
                     ATVATLTSAKYAWLSNLAVTAVEILGGFALGALVGVALAVVFCWSPLVSLLLLPLFVT
                     LNMVPKVALGPLFIVWFSYGIFPNILIAFSICFFPILLTTARGLSEVEPD"
     gene            complement(674..1315)
                     /locus_tag="VGA15_23055"
     CDS             complement(674..1315)
                     /locus_tag="VGA15_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9590610.1"
                     /translation="MPKQRSNGTQRRDPIATRKKLLTAARREFAQNGLAGARVDEIAA
                     RAGVNKQLVYHYFGDKDALYLAVLEWVYEEIRTQERKLNLEGLPPEQAIKRLIESSFD
                     HLAAHPDFIVLLNDENRGGARHVRGSSKIEAMHSPLVSMVSKILKQGVRAGTFRKGID
                     PVHLYISIAGLSYFFFSNTPTLSAIFGKDLASPAAKRTRRKHVVDLVMQALRP"
     gene            1452..2466
                     /locus_tag="VGA15_23060"
                     /pseudo
     CDS             1452..2466
                     /locus_tag="VGA15_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495663.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2841..4775
                     /locus_tag="VGA15_23065"
     CDS             2841..4775
                     /locus_tag="VGA15_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX9590611.1"
                     /translation="MTQTGASETAKDASASVVAQHAATLKALPFSDTRDFEEASRGFL
                     GTLENARITSAQGRVVWSLEPYGFLSEEKAPPTVDPSLWRQSRLNMSHGLFEVMPGVY
                     QVRGLDIANMTLIEGERGVIVVDTLTSIEGARAAMELYFQHRGKRTVAAVIFTHTHTD
                     HWGGARGVLDDAALAGGNIPIIAPNLFMEHAVSENIIAGPAMLRRAQYQFGPFLAKGS
                     RGQVDCGLGKSMAAGSVALLRPSDLIMTTGDKRTIDGLEFEFQMAPNSEAPAEMHFFV
                     PRYKLLNLAENCTHNFHNLLPFRGADVRDALAWSKYLGEALQMWGGKADAMCGQHHWP
                     VWGHERIDTMIRQQRDLYKFAHDQTIRLMNHGLTATEIAETIKLPASLDGAWHARGYY
                     GHIRHNVKAIYQKYLGWYDANPVHLDPLPPVEAGKKYVEYMGGAEAILARAARDFEKG
                     EFRFVAQAVSHLVFADPDNQAARALLADSFEQLGYAAESATWRNAYLFGAQELRQGMP
                     KAPPRPPMPRETLAALRTEQLWDVLGVRLNGPKAEGKHIVLNWRFTDTNETFVLTLEN
                     CALTFIAGARAAAADASFTLARGVLDEVIAKQTTFPEAVTSGKINFTGDPTRLGELMT
                     LMDEFARMFEIVEPRRVVVT"
     gene            5096..>5536
                     /locus_tag="VGA15_23070"
     CDS             5096..>5536
                     /locus_tag="VGA15_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEX9590612.1"
                     /translation="MTPTRRHVLAAFAAAAATKAASAQGGSLQEIGLQLYTVRDLLRT
                     DFEGTLRAIARLGYQEVEFAGLLGPDLKQTRNLLGELGLSAPSLHMDYNSLRKNTEAS
                     FETATALAAKFVVCPSLDQSERQTIDDWKRVCENLNSIGELATHS"
BASE COUNT         1157 a         1633 c         1715 g         1031 t
ORIGIN      
        1 gatcaggctc gacctcgctc agtccgcgcg cggtggtcag caggatcggg aagaagcaga
       61 tactgaaggc gatcaggatg ttcggaaaaa tcccgtagct gaaccagacg atgaacagcg
      121 ggccgagcgc cactttcggg accatgttca gcgtgacgaa caagggcagc agtagcaggc
      181 tcaccagcgg cgaccagcag aacacgaccg ccagcgccac gccgacgagc gcgccgagcg
      241 cgaagccgcc gaggatttcc accgctgtca cggcgaggtt ggaaagccat gcatatttcg
      301 cggatgtcag cgtcgcgacg gtggccagtg gcgagggcag tacgaatttc ggcacatgga
      361 aagcgtcgac cagaacctgc cacagcacga tcacggcaag atgcacgatc aggatgatcg
      421 caaacgatcg ggcggaacgc attccgtcgc tggacaccat gtgctcctct tgcgctcgtc
      481 ccaaccggag taggtccaat ctattgcgtt accctgtatc tttcaaccgt ctggttgatt
      541 aaaccaatca ttgcgagcca acgggtcgcg cgaatgcgcg cccgatgacg ggctccgcga
      601 agcaaatcca ttacctgagg ggccgctgga ttgcttcgtc gcttcgctcc tcgccatgac
      661 ggtgagccat gcatcaaggc cgcagcgcct gcatcaccag gtcgacgacg tgtttgcggc
      721 gggtccgctt agccgccggg ctggcgaggt ccttgccgaa gatcgccgac aaggtcggtg
      781 tgttcgagaa gaagaagtag ctgagacccg cgatcgaaat gtagaggtgc accgggtcga
      841 tgcctttgcg gaaggttcca gcccgaacgc cctgcttcag gatcttcgag accatgctga
      901 ccaggggtga atgcatggcc tcgatcttac tggagccgcg gacatgccgg gcgccgccgc
      961 ggttttcatc gttgagcagc acgatgaaat cgggatgcgc cgcgaggtgg tcgaatgaac
     1021 tctcgatcag cctcttgatg gcctgttcgg gaggcagacc ctccaggttc aacttgcgct
     1081 cctgggtccg gatttcctcg tagacccatt cgagtacggc aaggtagagt gcgtccttgt
     1141 caccgaaata atgatagacc agttgcttgt tgacgcctgc acgcgccgcg atttcgtcga
     1201 cccttgcgcc ggcgaggccg ttttgtgcaa attcccgccg cgccgccgtc agcagctttt
     1261 tgcgggtcgc gattggatcg cgccgctgcg taccatttga tcgttgttta ggcatttcca
     1321 accagacagt tgacaattcc agacagttga caattcggtc cgtcccttgt tgcattggta
     1381 gacgcaacga ccggtaacga caaggccgtc ggaaagtccg gccgggccgg gccgcaaacg
     1441 aggagggtgc catgaaagct tttgggatca aagcttttgg gatcgcggaa ctggcgctcg
     1501 cattgctggc gccttcgttg cctgccgggg ccgccgagtc cgtcaatctc attctgaact
     1561 ggacgccgac cgcggaccat tcgccattct actacgccaa ggcacaaggc tggtacgaga
     1621 aagccggtat cgatcttaac atcgaggttg gcaagggttc cggcgtttcg tcgttgaagg
     1681 tgggctccgg cggctcgccg ttcggcatcg cggatctcgc caccatgctg gtggcgcgcg
     1741 gcaagggcgc cgatgtcgtg gcgctgatga gcatctacgc caataccggc cagaccttct
     1801 attggctgaa aagctacggc gttaacggcg caaaagattt tccgggccac aagatcggca
     1861 atccgccggg cgacgcctcg cgcgtgatgt ggccggcgtt cgccaaggcg gccgggatcg
     1921 cacccgacgc ggtcggcttt gtcaatgtcg gaccgaccgc caagatcgcg gcgcttaaaa
     1981 gtcacaccgt tgatatcatc agcgatttct acaacgagca tgatttgaag gtgatcgagt
     2041 tcggcagcga cctcgggttt gtcaattgga aggacatcgg tctcaatccc tacggcaatt
     2101 cgttgatcgt caacggtgcg ttcctggaga aaaatccaaa acttgccgag gatttcgtaa
     2161 aaatcagcca gaaggcgttt gcggcctgcg tcgccgacgt caccccctgc ctgaaggcgc
     2221 tgctcgacca ggtgtcgggt ctcgacaagg aaaaccagga gcgccagtgg gagcgcatca
     2281 agttcctgat gaccgacgat tttacgacga ccaaggcgct gggctggatc gacgccgagc
     2341 ggatgaagaa ggattacgaa ctggtgcaga cctatctcgg catggagaag ccgttcgccg
     2401 tggaaaccgc gtttacaaca aaactgctcg tccatcaaga tggacgcgag caaggtcaag
     2461 aagtgagatg gatcggcgct ctgcatagag gcgaagctgc tttgccactt gtcggttgct
     2521 agagcccgat ccaggagcaa tagagccccg gagtgcagga gttcagccac ctctcccaca
     2581 gggagaggtc ggatcgttct tgcgatccgg gtgaggggtt acggcctaac gagagagcgt
     2641 aacccctcac cccaaccctc tcccatcgaa gtcggatata tccgacttcg acaatttgaa
     2701 atgtccaact cgggtagacc cgagttggat ggagagggag cgcgctgccg attcactgcg
     2761 ccgtcatgcc gaaggctttg acgaccgacg tcagctcgtc ataatccgcc ctttcgcacc
     2821 atgcagggga gaacaactca atgacccaaa caggcgccag cgaaacagcc aaggacgcct
     2881 cggcatcggt agtcgcgcag catgcggcga cgctgaaggc gctgccgttc tcggacacgc
     2941 gcgatttcga ggaggcgtcg cgcggttttc tcggcacgct ggaaaatgcg cggatcacgt
     3001 cagcgcaggg cagggtggtc tggagcctcg agccttacgg ctttctttcc gaagaaaaag
     3061 ctccgccgac ggtcgacccg agcctgtggc ggcaatcgcg gctcaacatg agccacggac
     3121 tcttcgaggt gatgcccggc gtctatcagg tcagggggct cgatatcgcc aacatgacgc
     3181 tgatcgaggg cgagcgcggc gtgatcgtgg tggacacgct gacctcgatc gaaggcgcgc
     3241 gggccgcgat ggagctctat ttccagcatc gcggaaaacg caccgtggcc gcggtgatct
     3301 tcacccacac ccataccgac cattggggcg gcgcgcgcgg cgtgctcgat gacgcggcgc
     3361 tggcgggcgg caacatcccg atcatcgcgc ccaatctgtt catggagcat gcggtttcgg
     3421 aaaacatcat cgcaggtccc gcgatgctgc ggcgcgcgca atatcagttc gggccgttcc
     3481 tggccaaggg ttcgcggggg caggtcgatt gcggcctcgg caagtcgatg gcggcgggct
     3541 cggtggcgct gttgcgcccc tcggatctca tcatgacgac cggcgacaag cgcaccatcg
     3601 acgggctcga attcgaattc cagatggcgc cgaacagcga agcgccggcg gaaatgcact
     3661 ttttcgttcc gcgctacaag ctcttgaatc tcgcggaaaa ctgcacccat aatttccaca
     3721 atctgctgcc gttccgcggc gccgacgtgc gcgacgcgct ggcctggtcg aaatatctcg
     3781 gcgaggcctt gcagatgtgg ggcggcaagg ccgatgcgat gtgcggccag caccactggc
     3841 cggtgtgggg tcacgagcgc atcgacacca tgatccgcca gcagcgtgat ctctacaagt
     3901 tcgcccatga ccagaccatc cgcctgatga atcacgggct cacggccacc gaaatcgcgg
     3961 aaacgataaa actgccggcg agccttgacg gcgcgtggca cgcacgcggg tattacggcc
     4021 acatccggca taacgtcaaa gcgatctacc agaaatatct cggctggtac gacgccaatc
     4081 ccgtccatct cgatccgctg ccgccggtcg aagccggcaa aaaatacgtc gaatatatgg
     4141 gcggcgcgga ggcgatcctg gcgcgcgcgg cgagggattt tgaaaaaggc gaattccgct
     4201 tcgtggcgca ggcggtgagc catctggtgt tcgccgatcc cgacaatcag gccgcccgcg
     4261 cgctgctcgc cgatagcttc gagcagctcg gctacgccgc cgagagcgcg acctggcgca
     4321 acgcctatct gttcggcgcg caggagttgc ggcagggcat gccgaaggcg ccgccgcggc
     4381 cgccgatgcc gcgcgaaacg ctggccgcgc tgcgcaccga gcagttatgg gatgtgctcg
     4441 gcgtccggct caatggcccc aaggccgaag gcaagcacat cgtgctcaac tggcgtttta
     4501 ccgatacaaa cgagacgttt gttctgacgc tggaaaactg cgcgctgact tttatcgcgg
     4561 gcgcgcgggc ggccgcggcc gacgccagtt ttactttggc gcgcggtgtg ctcgacgagg
     4621 tcatcgcaaa acagacgaca tttccggaag ccgtgacaag cggcaagatc aacttcaccg
     4681 gcgatccgac gcggctcgga gagctgatga cgctgatgga cgaattcgcg cgcatgttcg
     4741 agattgtcga gccgaggcgg gtggtggtta cttgacgcgt cattgcgagc caacgggtcg
     4801 gcgcgaagcg ccgcccgatg acaggctccg cgaaacaatc cataacctgt cgcgtgaact
     4861 ccggattgct tcgtcgcctc gctcctcgca atgacgttcg caaacattgt agcccggatg
     4921 agtgaagcga tatccgggaa cgaacgtgac gccgccccgg atctcgcttc gctcatccgg
     4981 gctacgctcg cttcaaaccg tcattccggg gcgatgcgaa gcatcgagag agtcggggcg
     5041 caaaagcagg atagcatgca tgctttaagg gctttcggcg caggagaagt cacagatgac
     5101 accaaccaga cgccatgttt tggcagcttt tgctgctgcc gctgctacca aagcagcctc
     5161 agcacagggt ggatctctgc aggaaatcgg gctccaattg tacaccgttc gcgatctgct
     5221 gcgcacggat tttgaaggta ccttgcgagc cattgcccga cttggctatc aagaagttga
     5281 gttcgctggc ctcctcggtc ccgacttgaa acagacaaga aacctgctcg gggaattggg
     5341 tctcagcgcc ccatcgttgc acatggacta caacagcctg cgcaaaaata cagaagcgtc
     5401 attcgagacg gcaacggcat tggcggcgaa gtttgtggtt tgcccaagcc tggaccagtc
     5461 ggagcgacaa acgatcgatg attggaagcg cgtttgcgaa aacctgaact ccataggtga
     5521 actggcgacg cattcg
//
LOCUS       DASWGM010000843         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2326689,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000843 DASWGM010000000
VERSION     DASWGM010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326689"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..479
                     /locus_tag="VGA15_23075"
     CDS             255..479
                     /locus_tag="VGA15_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX9590613.1"
                     /translation="MKIEIKKIGNSDGLLLPRELMQRLDLKRGQELHITELAGGGFQA
                     MPYDPDFEKTMEIADQIMDKYRDTLAALAK"
     gene            492..896
                     /locus_tag="VGA15_23080"
     CDS             492..896
                     /locus_tag="VGA15_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX9590614.1"
                     /translation="MRESKEPLWITYEQAIAIHSRQLRRFGGAPGLRDEGMLRSALER
                     PINKWRYEQSDMAELAAAYAFGLAKNHAFVDGNKRIAFMAMMAFLLKNGVAFDPEPAQ
                     STSIILSLAAGEVSEESLTRWIRDNWPHEPGA"
     gene            993..1145
                     /locus_tag="VGA15_23085"
     CDS             993..1145
                     /locus_tag="VGA15_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEX9590615.1"
                     /translation="MAEHNEVAYTTADGNDYPAHEQTYEGFVMLVKYGTAAVIFIVAM
                     MAIFLT"
     gene            1284..2411
                     /locus_tag="VGA15_23090"
     CDS             1284..2411
                     /locus_tag="VGA15_23090"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX9590616.1"
                     /translation="MKIAVAKEIDPSEPRVAASPDTVKKFKALGVDIAIEPGAGVKSG
                     LPDSEFTAAGATVSADALRDADIVIKVKRPEASELANYKRGTLVIAIMDPYGNDAALK
                     AMADAGISAFAMELMPRITRAQVMDVLSSQANLAGYRAVIEAAEAFGRAFPMMMTAAG
                     TIPAAKVFVMGVGVAGLQAIATARRLGAVVTATDVRPAVKEQVESLGAKFLAVEDEEF
                     KQAETAGGYAKEMSKEYQAKQAALTAEHIKKQDIIITTALIPGRPAPRLVTADMVKSM
                     KPGSVLVDLAVERGGNVEGAKAGEVADVGGIKIVGYTNVAGRVAASASSLYARNLFSF
                     IETLVDKSTKALAVKWDDELVKATALTKDGAVIHPNFQPKA"
     gene            2423..2740
                     /locus_tag="VGA15_23095"
     CDS             2423..2740
                     /locus_tag="VGA15_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEX9590617.1"
                     /translation="MDHIAQAVDPFVFRLSIFVLAVFVGYFVVWSVTPALHTPLMSVT
                     NAISSVIVVGALLAVGVAMVGSGGAWARGFGFVALIFASVNIFGGFLVTQRMLAMYQK
                     KKK"
BASE COUNT          580 a          858 c          906 g          544 t
ORIGIN      
        1 cttcgccgag cgctatctgg cgatgctggc ggccggcggc accaagcatt attccgaact
       61 gctcaagccg ttcgggctgg atgccaagga cccgaaattc tgggatggcg ggctgtcggt
      121 gatcgcgggc atgatcgacg aactggaagg gatggattaa ataatccgta ttacaagcgt
      181 tgaacgcagc caaagggcat gttatacaga tgtacaacat gccctttggc gttgctgcgc
      241 gaggagaact gaccgtgaaa atcgaaatca agaagatcgg taattccgat ggcctcctat
      301 tgccacgtga attgatgcag cggctcgacc tcaagcgggg ccaggaactg cacattaccg
      361 aactcgccgg aggcggattt caggccatgc cttacgatcc cgacttcgag aagaccatgg
      421 agatcgcgga ccagattatg gacaagtacc gggacacgct cgcggcgctt gcaaaataag
      481 cagtgcaacc tatgagagag tcgaaagagc cgctttggat cacctatgaa caggccattg
      541 cgatccatag ccgccaactg cgccgcttcg gcggagcgcc cggcttgcgc gacgaaggca
      601 tgctgcgatc cgcgcttgaa cgtccgatca acaaatggcg ttacgaacaa tcagacatgg
      661 ccgagcttgc ggcagcctat gcgtttgggc tcgcgaaaaa tcacgccttc gtcgatggta
      721 acaagcgcat cgccttcatg gccatgatgg cctttctgct taagaacggc gttgctttcg
      781 accctgaacc cgcacagtcg acatccatca tcctctccct tgcggccggc gaagtcagcg
      841 aggaaagcct tacccgctgg atcagggaca attggccgca cgagcccggc gcgtaaaaga
      901 cacggccaag cgcacgaccg cgcatcgccg ttgccctctc gttgggtttg cggtaattgc
      961 acgccagatc gagcattcac cgggggacac cgatggcaga gcataacgaa gtggcctaca
     1021 cgaccgctga cggcaacgat tatccggccc atgagcagac ctatgagggt ttcgtcatgc
     1081 tggtgaaata cggcaccgcc gccgtcatct tcatcgttgc catgatggcg attttcctga
     1141 cctgatccat cgccggccgc acaccgccaa ccggcggcgg tatcagaaga tcgcacgcat
     1201 tccgcttgca aaaccggtat ccatctctgc caaataggcg ctagtttgcg cgcgcgcgtg
     1261 cttcccgcgc cgccggaggc cccatgaaaa ttgccgttgc caaggaaatc gatccgtccg
     1321 agccgcgggt cgcggcttcg cccgacaccg tgaaaaaatt caaggcgttg ggcgtcgata
     1381 tcgcgatcga gccgggcgcg ggcgtcaagt cgggtctgcc ggattccgaa tttaccgcgg
     1441 ccggcgccac cgtcagcgcc gatgcgctca gggacgccga cattgtcatc aaggtgaagc
     1501 ggcccgaggc gagcgagctt gccaattaca agcgcggcac gctggtcatc gccatcatgg
     1561 atccctacgg caacgacgcg gcattgaagg cgatggcgga tgccggcata tcggcgttcg
     1621 cgatggagtt gatgccgcgc atcacccgcg cccaggtgat ggatgtgctg tcgagccagg
     1681 ccaatctcgc cggttatcgc gcggtgatcg aggcggcgga ggccttcggc cgcgcctttc
     1741 cgatgatgat gaccgcggca ggcacgatcc cggcggcgaa agtgttcgtg atgggcgtcg
     1801 gcgtcgccgg cttgcaggcg atcgcgaccg cgcggcggct tggcgccgtc gtcaccgcga
     1861 ccgacgtgcg ccccgctgtg aaagagcagg tcgagagtct cggcgccaaa ttcctcgcgg
     1921 tcgaggacga ggagttcaag caggccgaaa ccgccggcgg ctacgccaag gaaatgtcga
     1981 aggagtatca ggccaaacag gccgcgctga ccgccgagca catcaagaag caggacatca
     2041 tcatcaccac ggcgctgatc ccgggccggc ccgcgccgcg ccttgtcacc gcggacatgg
     2101 tgaagtcgat gaagccgggc tcggtgctgg tcgatcttgc ggtcgaacgc ggcggcaatg
     2161 tcgagggcgc caaggccggc gaagtcgccg atgtcggcgg catcaagatc gtcggctaca
     2221 ccaacgtggc cggccgtgtc gcggcctcgg cctcgagcct ttatgcgcgc aacctgtttt
     2281 ccttcatcga gacgctggtc gacaaatcga ccaaggcgct cgcggtgaag tgggacgacg
     2341 aactggtcaa ggcgaccgcg ctgaccaagg acggcgccgt cattcatccg aattttcagc
     2401 cgaaagcata aggagagccg tcatggatca tatcgcgcag gccgtcgatc ccttcgtgtt
     2461 ccggctgtcg attttcgtgc ttgccgtgtt cgtcggctat ttcgtggtgt ggtcggtgac
     2521 gcctgcgctg catacgccgc tgatgtcggt gaccaacgcg atctcctcgg tgatcgtggt
     2581 cggcgcgctg ctggccgtgg gcgtcgcgat ggtcggcagc ggcggggcct gggcgcgcgg
     2641 tttcggcttc gtcgcgctga ttttcgcgtc ggtgaatatt ttcggcggct ttctggtgac
     2701 gcagcgcatg ctcgcgatgt accagaagaa aaagaagtga cggcgactat gaatcttttt
     2761 cagaagatat cgtcgtcatt gccgggcttg acccggcaat ccatcgcgct tcgcaagatt
     2821 ctttttattc agatggatgc gcgggtcgag cccgcgcatg acggagtgcg cgcatcatga
     2881 acgccgat
//
LOCUS       DASWGM010000844         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2709695,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000844 DASWGM010000000
VERSION     DASWGM010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2709695"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1528)
                     /locus_tag="VGA15_23100"
     CDS             complement(<1..1528)
                     /locus_tag="VGA15_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9590618.1"
                     /translation="MTEPDVLIVGAGPVGLTLAIDLAWRGIDVTVVETRGRAAPPEPK
                     CNHVAARTMEIFRRLGLAEKVRDAGLPADYPHDISYRTTFTGPELARIPIPCRRDRFT
                     RTEGPDCNWPTPEPPHRINQIFLEPILFEHAAAQPRIRIINRTSVENVVVADRSAHVT
                     LRDLDAGAVRRLSCRYLIGCDGARSVVRKAIGAELAGDAVVQRVQSTFIRAPGLIDRQ
                     QHARAWGTGVINPRRSGMVYAIDGRERWLVHNYLKPDETDFEQVDRDACIRTILGVGA
                     DFDYDIISREDWYGRRLIASRFRHACAFIAGDAAHIWVPYAGYGMNAGIADAMNLSWL
                     LAAHLNGWAPASILDAYEAERWPITSQVSRFAMSHAEAEIRRRGAVPDEIEEAGPRGE
                     DARREVGRLAYEINVQQYACAGLNFGTYYDRSPIIAYDGAEHPAYTMDSYTPSSVPGC
                     RTPHLWSGNGGSLYDAMGPEFTLLRFDPALDVVALEAAARNRGVPLKILDIEPPCTAI
                     F"
     gene            complement(1584..1937)
                     /locus_tag="VGA15_23105"
     CDS             complement(1584..1937)
                     /locus_tag="VGA15_23105"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9590619.1"
                     /translation="MSLTRRAALAGCGGGLAMLTRLPAVSRPAFAQPAFAQPALAQAA
                     LGVVAPAGVPPAIVDPLAAQIAKLMADPITREKLTTIALEPLASSTPDSFAAFIKTEV
                     DRWAGIVKNSGAEPE"
     gene            2237..>3528
                     /locus_tag="VGA15_23110"
     CDS             2237..>3528
                     /locus_tag="VGA15_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9590620.1"
                     /translation="MTLIAIATMLATASAFAQGAGTAGPDMAESGAAPHPAKPVKPAK
                     PAAASTPESRSAAALALSHEPTFDEGTAQRIKEAALSYSDLAVRGGWPIIPAEAKFAS
                     GTQGPHDDLLRQRLLISGDLDPDKASGAYDDVLAESVKRFQARHGLAPTGTMTPRTLA
                     ALNVPVQKRIKQLEASLERLENMNFAFGQRYVVVNLPAAFAEAVENDHVVRRYRVIVG
                     KTEKPSPMLTAEITSVNLNPTWTVPSSIAKTEISAHMRKDPGYLARMHMEVLDAHEAP
                     IDPRSVDWSGAHTPNFTVRQQNGGWNALGAVKIDMPNPYSVYMHDTNQRNLFSDDYRF
                     DSHGCSRVDNVRDLATWLLQEEMPKWNRAAIDAAIATGQREDIALPKKVPVAWIYLTG
                     WMTKDQTVQFRNDIYDQDEQLLEATAEEAAFFSQAANHP"
BASE COUNT          707 a         1161 c         1067 g          593 t
ORIGIN      
        1 agaaaatggc ggtacatggc ggctcgatgt cgaggatttt tagcggcacg cccctgttgc
       61 gagccgcagc ctccagcgcg acaacatcca gcgccggatc gaacctcagc agcgtgaact
      121 ccggccccat tgcatcgtaa agcgagccac cattcccgct ccacagatgc ggggtgcggc
      181 aacccggcac gctcgacggc gtgtagctgt ccatcgtata ggccggatgt tcggcaccgt
      241 cataggcaat gattggcgaa cgatcgtagt aggtgccgaa gttgagaccg gcgcaggcat
      301 attgctggac gttgatctcg taggcgagac ggccgacttc ccgccgcgca tcttcgcctc
      361 tcggtcccgc ctcctcgatt tcatcgggca ccgcgccacg gcggcgaatt tcagcttcgg
      421 cgtgcgacat tgcgaatcgc gacacctggc tggtgatcgg ccaacgctcc gcctcatagg
      481 cgtcgagaat cgaggccggc gcccagccat tgagatgggc ggcaagcagc caggacaggt
      541 tcatcgcgtc ggcaattccg gcattcatgc cgtagccggc atagggcacc cagatatgcg
      601 ccgcgtcacc ggcgatgaac gcacaagcgt ggcgaaaccg gctcgcgatc agccggcgcc
      661 cgtaccaatc ctccctggaa atgatgtcgt agtcgaagtc cgctcccacg ccgagaatgg
      721 tccggatgca ggcgtcgcga tcgacctgtt caaaatcggt ctcgtcgggt ttcagataat
      781 tatgcaccag ccatcgttcg cggccatcga tcgcatacac catgccggag cgcctggggt
      841 tgatgacacc ggtgccccag gcgcgcgcgt gctgctggcg atcgatcagg ccgggagcgc
      901 ggataaaggt cgactgcacc cgctgcacca cggcgtcgcc ggccagctcc gcaccgatcg
      961 ccttgcggac caccgagcgc gcgccgtcgc agccgatcag atagcgacag ctcaaacgcc
     1021 tgaccgcgcc ggcgtcgaga tcgcgcaagg tcacatgcgc tgacctatcc gccaccacca
     1081 cgttttcgac cgacgtccgg ttgatgatgc ggatccgtgg ctgggcggcg gcatgctcga
     1141 acaggatcgg ctcgagaaat atctggttga tacgatgcgg cggctccggg gtcggccagt
     1201 tgcagtccgg cccctccgtc ctggtgaatc gatcgcggcg gcagggaatt ggaatccgcg
     1261 ccagctccgg acccgtaaac gtggtgcgat aactgatgtc gtgcggataa tccgcgggca
     1321 atccggcgtc gcgcaccttt tcagcaaggc caagccgccg aaaaatttcc atggtgcggg
     1381 cggcgacatg gttgcatttg ggctccggag gcgcggcgcg gccgcgcgtc tccacaaccg
     1441 taacatcgat ccctcgccac gccagatcga tcgcgagcgt gagcccgacc ggtcctgcgc
     1501 caacgatcag tacatccggc tcggtcattt cgggcatcga tcgcgcttgg cgcggaaaac
     1561 ttgacctgag gacatggttc tcgtcattcc ggctcggcgc ccgaattttt gacgatgccg
     1621 gcccagcgat cgacctcggt cttgatgaag gccgcaaaac tgtccggcgt cgaggaggcc
     1681 agcggctcca gtgcaatcgt cgtaagtttt tcgcgggtta taggatcggc catcagcttg
     1741 gcaatctgcg cagccaatgg gtcgacgatc gccggcggca cgccggcggg cgcgacgacg
     1801 cccaaggctg cttgggccaa ggctggttgg gcaaaggctg gttgggcaaa ggctggtcgg
     1861 gaaacggctg gcagcctcgt cagcatggcc agcccgccgc cacatccggc gagcgccgct
     1921 cggcgcgtca acgacataga tcctcccaaa cgcacgctta ttttctagcc ggcgggtgcg
     1981 ctcaccaacc acattagctg ccgcggcgac atatcgaaag ccacgaaaat ccaatacggc
     2041 acttgcggga acgcaatagc cgcaatacca acctaatcgg aacgcaactc tcacaagcac
     2101 catttacgaa aacccaattg cggatcaggt ccgatcccgt tcgtccggct ccaacggccg
     2161 ggcttgttga tttcaacgcc agcgccatca gggcgcgtcc caagggcacc caacaggggc
     2221 atccagcatg cgaagaatga cactcattgc cattgccacg atgcttgcga ccgcgtcggc
     2281 gtttgcccaa ggcgcaggca ccgcgggccc ggatatggcc gagtccggcg ccgcaccgca
     2341 cccggccaaa cctgtcaagc cagccaagcc ggcagccgca tccacccccg agtcacgctc
     2401 cgccgcggcc ttggcgctgt cgcacgagcc gaccttcgac gaggggactg cgcagcggat
     2461 caaggaggcg gcgctgagct attcggacct tgcagtgcgc ggcgggtggc cgatcatacc
     2521 ggccgaggcc aaattcgcat ccggcaccca gggaccgcat gacgatctgc tgcgccagcg
     2581 cctgttgatc agcggcgatc tcgacccgga caaagcgtcc ggtgcctatg acgacgtcct
     2641 cgccgaaagc gtaaaacgtt ttcaggcgcg tcacggcctc gcgccgacgg gaacgatgac
     2701 gccacgcacc ctcgccgccc tcaatgtccc cgtgcagaaa cggatcaagc agctcgaggc
     2761 ttcgctggag cgcctcgaga atatgaattt cgcattcggc cagcgctacg tggtggtcaa
     2821 cctgccggcg gcatttgccg aggcggtgga aaacgatcat gtggtgcgac gttatcgcgt
     2881 gatcgtcggc aagaccgaaa agccgtcgcc gatgctgacc gctgaaatca ccagcgtcaa
     2941 tctcaacccc acctggaccg tcccgtcatc gatcgcgaaa accgaaatct cggcgcatat
     3001 gcgcaaggat cccggctatc tcgcccgcat gcatatggag gtgctcgatg cgcacgaggc
     3061 tccgatcgat ccgcgatcgg tggattggtc cggtgcgcac accccgaatt tcacggtgcg
     3121 ccagcaaaac ggcggctgga atgcgctggg tgcggtgaag atcgacatgc caaatcccta
     3181 ttcggtctac atgcacgaca ccaatcagcg caatctgttc agcgacgact accgcttcga
     3241 ctcgcacggc tgctcgcgcg tcgacaacgt ccgcgacctc gccacctggc tgctgcagga
     3301 ggagatgccg aaatggaacc gcgccgccat cgacgcggcc atcgccaccg gccagcgcga
     3361 ggatatcgcc ctgcccaaaa aagtcccggt ggcctggatc tatctcacgg ggtggatgac
     3421 caaagatcag acggttcagt tccgcaacga catctacgac caggacgaac aacttctgga
     3481 agccaccgcc gaagaggccg cgttcttcag tcaggcggcc aatcatcc
//
LOCUS       DASWGM010000845         3502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2618736,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000845 DASWGM010000000
VERSION     DASWGM010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2618736"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(515..622)
                     /locus_tag="VGA15_23115"
                     /pseudo
     CDS             complement(515..622)
                     /locus_tag="VGA15_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498139.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
     gene            912..1376
                     /locus_tag="VGA15_23120"
     CDS             912..1376
                     /locus_tag="VGA15_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9590621.1"
                     /translation="MATTLTINGEAKSFDAPADMPLLWVLRDVLGMTGTKFGCGIAQC
                     GACTVHIDGKPVRSCMLPVSAVRDRAVTTIEGIGASPAGAKVQKAWLDLEVIQCGYCQ
                     SGQIMSAAALLAATPNPDDSDIDAAMAGNICRCGTYVRIRAAIKQAASGRQS"
     gene            1386..>3502
                     /locus_tag="VGA15_23125"
     CDS             1386..>3502
                     /locus_tag="VGA15_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9590622.1"
                     /translation="MTIAEKISAEQTSDPVSRRALLSGGLAGGFLLAFHLPVRALVPN
                     EPVQPPDVTDGKFAPNAFIRIDVTGHTTLVMPQVEMGQGVYTAIAMILAEELDADFSQ
                     VTLQHAPPNDRLYGNPTFGLQVTGNSNSIRAFWKPLRNAGAGARAMLVQAAAQQWQVE
                     PASCSTANSEVTHKESGRKLSYGALAAAAGSQTPPKDVALKDPKDFALIGKPLKRFDT
                     PDKVNGKAVYGIDAMLPGMKFATLAACPVFGGKVGKVDDSAAKKIPGVQKIVVLDDLV
                     AVVGDHMWAAKKGLDALVIDWDEGANARISSKDIWNDLRAASEKDGVVARSEGDIAKG
                     LATGERMDAAYELPFLAHATMEPLNATVHLTPDSCEIWTGTQIVSRVQAEAAKAAGLP
                     VNKVIVNNHLLGGGFGRKLEPDMVVAAVRIAKQVDGPVKVVWTREEDIQHDIYRPVYR
                     DTIAATLSDGKIVGWKYRITGSAVIARWLPPAFQKGIDIDAIDSAVDMPYDIPNIHVE
                     YVRAEPPAVPTGFWRGVGPNNNVFAVECFMDELARKAGKDPIAFRLAMLGKNPRMQAA
                     LNLAAEKSNWGQPLPARVGRGVCVQPSFGSFIATVVEAEVDEAGEVHLRRVTSAVDTG
                     IAVNPDTIMAQLEGGLIFSLTAALYGEITIDKGRVQQSNFHDYRMLRIDQAPKIEVHV
                     IKSGEPPGGIGETGSTAGPPALR"
BASE COUNT          694 a         1097 c         1089 g          622 t
ORIGIN      
        1 caacttgaca ccagcgtcgg ggggtcagga ccacacgact tcgccgtccg caagggttgc
       61 gctctcgtca gcagcgccgc ctgcgtccac cgcattccgc cccgcgtccg tgacgatcgc
      121 gagccgcccc tctgtgggac ggaacggcag cgggtataga agtgatttgg gtatagtggg
      181 aacagaaata tttttgcgaa aggggctgga cacaaaaatc gccgagcagc ccgtcgggca
      241 aatcagtcga cctgtcgccg tagctcaaac ggcggacggt gtaacccgct tctttatcat
      301 cgatgaatgg tgggtgatga atagccaatt aagtgcaccg tcacagtaat tcctgaatga
      361 tccgctggct caatgttttt tcgctgatca tggtcgcaat catcggcgcg gagcccgtag
      421 cccgtagccc gtaggatggg ttgagccctt cgcgaaaccc atcgacgtcc cgaaaggaat
      481 ttgatgggta tcgctggcgc tccacccatc ctgcgagact ggcgcggcca tcgcagaaaa
      541 atccgacgcc ccgcttttcg ctggtcgcct cgcaatgcaa gtcgaccacg atggcatccg
      601 cggccgtccc taaggggcag ctccgagcct gaggtcgatc accgcgaccg cagatcttgc
      661 ttcctgaccc gcacttcggt ttggcaaatt gccgactgaa gctgcactgg aatgacggcc
      721 atcatcccat tagcccaaaa ccgatgcggt cattcagcac gattgcgtga agccgcgccg
      781 tctccttcac gaccagcgca aatcagcatt gcgaaaatat cctcgcgtca tatgtcaccg
      841 cggcgcgata tagtcttgct gacttcgcca ccgtggccgc cggtcacggc tgttaaattg
      901 gaggggcact catggcgacc acgctgacga tcaatggcga agcaaaatcg ttcgacgcgc
      961 cggccgacat gccgttgctg tgggtgctcc gcgacgtgct cggcatgacc ggcacgaaat
     1021 tcggctgcgg cattgcccag tgcggcgcct gcacggtgca tatcgacggc aagccggtgc
     1081 gctcctgcat gttgccggtg agcgccgtgc gcgatcgcgc cgtcaccacc atcgaaggca
     1141 tcggcgcgtc gccggccggc gccaaagtgc agaaggcctg gctcgatctc gaggtaatcc
     1201 agtgcggcta ttgccaatcg ggccagatca tgtcggccgc ggcgctgctc gcggccacgc
     1261 ccaatccgga tgattccgac atcgacgcgg cgatggcggg aaatatctgc cgctgcggaa
     1321 cgtatgtgcg gattcgcgcg gcgatcaagc aggccgcgtc cggtcgccag tcgtaggagc
     1381 gcgccatgac aatcgccgag aaaatttccg cagaacagac ttccgatcca gtttcacgcc
     1441 gtgcgctgct gagcggcggt ctggcgggcg ggtttctgct cgccttccac ctgccggttc
     1501 gcgcgctggt gccaaacgaa ccggtgcagc cgcctgatgt gaccgacggc aagttcgcgc
     1561 ccaacgcctt cattcgcatc gatgtaacag gccataccac gctggtgatg ccgcaggtcg
     1621 agatgggcca aggcgtctac accgccattg cgatgattct ggccgaagaa ctcgacgccg
     1681 atttctcgca agtgacgctg cagcacgcgc cgccgaacga cagactgtat ggcaatccca
     1741 cgttcgggct ccaggtcacc ggcaattcca attcgatccg ggcgttctgg aaaccgctgc
     1801 gcaatgccgg cgccggggca cgcgccatgc tggtgcaggc cgccgcccag caatggcagg
     1861 tcgagccggc gagttgctcg acggcgaaca gcgaggtcac gcacaaggaa agcggacgca
     1921 agctttccta tggcgcgctg gcggccgccg ccggcagcca gacgccacca aaggatgtcg
     1981 cgctcaagga cccgaaggat tttgcgctga tcggcaagcc gctgaagcgt tttgatacgc
     2041 ccgacaaggt caacggcaag gccgtctacg gcatcgacgc gatgcttccc ggcatgaagt
     2101 tcgcgacatt ggcagcctgc ccggtattcg gcggcaaggt cggcaaggtc gacgacagcg
     2161 ccgccaaaaa aattccgggc gtgcagaaga tcgtcgtgct cgacgatctg gtcgcggtgg
     2221 tcggcgatca catgtgggcg gccaagaaag gcctcgatgc gctggtgata gattgggatg
     2281 aaggcgccaa tgcccgcatc agctcaaaag acatctggaa cgatttgcgc gccgccagcg
     2341 aaaaagacgg tgtggtggcg cgatccgagg gcgatatcgc caagggcctt gccaccggcg
     2401 agcggatgga cgcagcctat gagcttccgt ttctcgcgca cgccacgatg gagccgttga
     2461 acgccacggt tcatctgacg cccgactcct gcgaaatctg gaccggcacc cagatcgtga
     2521 gccgggtgca ggcggaagcc gcgaaggcgg ccgggctccc cgtcaacaaa gtgatcgtca
     2581 acaatcacct gctcggcggc ggtttcggcc gcaagctcga gcccgacatg gtggtcgcgg
     2641 ccgttcgcat cgccaagcag gtcgacgggc cggtcaaggt ggtgtggacc cgcgaagaag
     2701 acatccagca cgatatctat cgtccggttt atcgcgacac cattgccgcg acgctttcgg
     2761 acggcaagat cgtcggctgg aaatatcgca tcaccggctc ggccgttatc gcgcgctggc
     2821 tgccgccggc gttccagaag ggcatcgaca tcgacgccat cgatagcgct gtcgacatgc
     2881 cctacgatat tccgaatatc catgtcgaat atgtccgggc cgaaccgccc gcggtgccga
     2941 ccggcttctg gcgcggcgtc ggtcccaaca acaatgtgtt tgccgttgaa tgtttcatgg
     3001 atgagctggc gcggaaggcg ggcaaggatc cgattgcctt ccgccttgcg atgctgggca
     3061 aaaacccgcg catgcaggct gcgctcaatc ttgcggccga gaaatccaat tggggtcagc
     3121 cgctgccggc gcgcgtcgga cgcggcgtct gcgtgcagcc gtcgttcggc agcttcatcg
     3181 ccaccgtggt ggaagcggaa gtcgatgaag ccggtgaagt ccatttgcgg cgcgtcacct
     3241 cggcggtcga taccggcatc gcggtcaatc ccgataccat catggcccag ctcgagggcg
     3301 gactgatctt cagcctgacc gcagcactct atggcgagat caccatcgac aagggacggg
     3361 tccagcaatc caatttccat gactaccgca tgctgcgcat cgatcaggcg ccgaagatcg
     3421 aggttcacgt catcaaaagc ggcgagccgc ccggcggtat cggcgaaacc ggctcgacgg
     3481 cgggaccgcc cgcgttgcgc aa
//
LOCUS       DASWGM010000846         5246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2710270,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000846 DASWGM010000000
VERSION     DASWGM010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2710270"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VGA15_23130"
     CDS             <1..480
                     /locus_tag="VGA15_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9590623.1"
                     /translation="VIFLENEMLYGHSGEVPKLDDYVIPIGKARIVRSGSHVTLVSWS
                     NGMSYALKAADELAKEGIEAEVIDLRTLRPMDTDTIVASVKKTGRAVTVEEGWQQNGV
                     GAEICARIMEHAFDYLDAPVARVSGKDVPMPYAANLEKLALPSVAEVVAAAKAVCYR"
     gene            484..789
                     /locus_tag="VGA15_23135"
     CDS             484..789
                     /locus_tag="VGA15_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5076 domain-containing protein"
                     /protein_id="HEX9590624.1"
                     /translation="MAGPKEQPLPPDVMDRDDATEVLRAFVVDGGLSIAFARAFEEPD
                     MWGLLLVDIARHAARAYARESDYTEEEALTRIVDMFEAEIARPTDVGSTTLRSQQGH"
     gene            846..1298
                     /locus_tag="VGA15_23140"
     CDS             846..1298
                     /locus_tag="VGA15_23140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX9590625.1"
                     /translation="MTIEPYHFEFMAEAIREAEISIAEGGLPIGAVLTRDRKIIARGH
                     NRRVQEQNVILHGEMSCMRNAGLIPFHDTVMYTTLSPCSMCAGAIGLFKLSLLVIGES
                     VTFPGSKDILAQFGIPYLDLDDERSVNLMKSWRSVPANERLWQGDIGN"
     gene            1404..2759
                     /locus_tag="VGA15_23145"
     CDS             1404..2759
                     /locus_tag="VGA15_23145"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEX9590626.1"
                     /translation="MPINILMPALSPTMEKGNLAKWLKKEGDKVKSGDVIAEIETDKA
                     TMEVEAVDEGTIAKILVPEGTQDVAVNDVIAVLAGDGEDVKAAGAGAASAPPKAAPTA
                     QTPAAAKPVPIAAAPAAKAAAPAAAAPAPQAAAPASQTNGHARIFSSPLARRLAKEAG
                     IELARINGSGPHGRVIARDVEEARSGKGLKAPAAAPAGAPSIAPSMSDKQVRALYEDG
                     SYEVIPHDGMRRTIAQRLTASVQTIPHFYLTIDCNIGKLVEAREEINAAAPKDKDGKP
                     AYKLSVNDFVIKALAIALQRIPNANVSWTDGGMLKHKHSDIGVAVAMPGGLITPIIRK
                     AETKPISIISAEMKDFAARARARKLKPEEYQGGTTAVSNLGMYGINHFTAVINPPHAT
                     ILAVGTGEERAIVKHGKIEVAQIMSVTMSCDHRAVDGALGAELIGAFKLLIENPVMMV
                     V"
     gene            2925..3341
                     /locus_tag="VGA15_23150"
     CDS             2925..3341
                     /locus_tag="VGA15_23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX9590627.1"
                     /translation="MRQSVPGQKRALAKALHAKATGAEIKLWRLLRSRRFASMKFRRQ
                     VPIGPWIVDFVSFEQRLIIEADGSQHAESENDKRRDYDLSERGFRVLRFWNNDVLMRP
                     QSVMEMIVDVVAQSPSPVCAPSGAQPPSPTRGEGSK"
     gene            3353..4771
                     /gene="lpdA"
                     /locus_tag="VGA15_23155"
     CDS             3353..4771
                     /gene="lpdA"
                     /locus_tag="VGA15_23155"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX9590628.1"
                     /translation="MPDTSFDVIIIGSGPGGYVTAIRAAQLGYKTAIVEKSYLGGICL
                     NWGCIPTKALLRSAEIFHYMQHAKDYGLSADNVSFDPKAVVQRSRGVAKRLNDGVGFL
                     MKKNKVTVIWGEASIDAPGKITVKKSAVEAPKGASGEGAYQAKHIILATGARPRLLPG
                     LEPDQKLIWTYFEAMVPEKMPKSLLVVGSGAIGIEFASFFHTMGADVTVVEVLPQILP
                     VEDAEIAGLARKRFEKQGIKILTNTKVTKLQKKTDSVLATIDDGKKPQTIEFDRVISA
                     VGVVGNIENLGLEKLGVKTERGVVVIDGYGKTSVPGIYAIGDVAGPPMLAHKAEHEGV
                     VCIEAIKGLHPHATDKNLIPGCTYCNPQVASVGLTEAKAKEGGREIRVGRFPFVGNGK
                     AIALGEDQGLVKVVFDKKTGQLLGAHMIGAEVTELIQGYVVAMNLETTEEELMHTVFP
                     HPTLSEMMKEAVLDAYGRVLNI"
     gene            4808..>5246
                     /locus_tag="VGA15_23160"
     CDS             4808..>5246
                     /locus_tag="VGA15_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX9590629.1"
                     /translation="MKDNDNLTIERPTFVTHLECAMEGDHYPADQVHNLSKAGKPLLV
                     RYDLAGVKQALTKDALAQRPADLWRYREMLPVRKIADIVSLGEVTTPLIRLPKLAAKL
                     GGGEIIVKDEGRLPTGSFKARGLVMAVSMGKALRIKHMAMPTNG"
BASE COUNT         1117 a         1554 c         1644 g          931 t
ORIGIN      
        1 gtgatcttcc ttgaaaacga gatgctgtac ggccattccg gcgaggtccc gaaactcgac
       61 gattatgtga tcccgatcgg caaggcgcgg atcgtgcgct ccggcagtca tgtcaccctc
      121 gtgtcatggt cgaacggaat gtcctacgcg ctgaaggccg ccgacgaact cgcgaaggaa
      181 ggcatcgagg ccgaggtgat cgatctgcgc acgctgcgcc cgatggacac cgacaccatc
      241 gtcgcctcgg tcaagaagac cggccgcgcg gtcacggtcg aggaaggctg gcagcaaaac
      301 ggcgtcggcg ccgaaatctg tgcccgcatc atggagcacg ccttcgatta tctcgatgcg
      361 ccggtggcgc gggtgtcggg caaggacgtg ccgatgccgt atgccgcgaa cctcgaaaag
      421 ctcgcattgc cgtcggtggc cgaggtggtc gccgccgcca aagccgtttg ttacaggtga
      481 accatggccg gtccaaagga gcagccattg ccgccagacg tcatggaccg cgatgatgcc
      541 acggaagtgt tgcgcgcgtt cgtcgtcgat ggcgggcttt ccatcgcatt cgcgcgcgcg
      601 ttcgaggagc cggatatgtg gggtctgctt ctcgtcgaca tcgcgcgcca tgccgcgcgt
      661 gcctacgcgc gcgagagcga ctacaccgag gaggaagcgc tgacgcgtat cgtcgacatg
      721 ttcgaagccg aaatcgctcg cccgaccgac gtgggcagca ccacgcttcg gtcgcaacaa
      781 ggtcactgac aatgccaacc cgtattccgt ttgttatcgc tagctcccga acgaaagcgt
      841 gacccgtgac tattgaaccc tatcatttcg aattcatggc tgaagccatt cgcgaagccg
      901 aaatcagcat cgcggagggc ggactgccga tcggcgcggt gctgacgcgc gacaggaaga
      961 tcattgcgcg cggtcataac cgccgggtgc aggagcagaa cgtcatcctt cacggcgaga
     1021 tgtcatgcat gcgcaacgcc gggctgattc cgtttcatga caccgtcatg tataccacgc
     1081 tgtcgccctg ttcgatgtgc gcgggcgcga tcggactttt caaactttcg cttctcgtga
     1141 tcggcgagtc cgtcacattt ccgggaagca aggatattct tgctcaattc ggcattccct
     1201 atctcgatct cgacgatgag cgatcggtca acctgatgaa gtcatggcga tctgtccccg
     1261 ccaacgagcg cctgtggcaa ggcgatatcg gcaattgaag cggatcttcg gtctttcatc
     1321 gggcgccgat ggcgcccaac ggtctttggt tggcttcctt aagggatgga agacaatgag
     1381 caagaatatc ctgtgaggtc agcatgccga tcaacattct gatgcccgcg ctgtcgccga
     1441 ccatggaaaa gggcaacctt gccaagtggc tcaagaaaga gggcgacaag gtcaaatccg
     1501 gcgacgtgat cgcggaaatc gagaccgaca aggcgacgat ggaggtcgag gcggtcgacg
     1561 agggtaccat cgcgaagatt ctggtgccag agggcaccca ggatgtcgcc gtcaacgacg
     1621 tgatcgcggt tttggccggc gatggcgagg acgtgaaggc ggcgggggcg ggggctgcca
     1681 gcgcgccgcc gaaggcagcg ccaactgctc aaacacccgc tgctgcgaag ccggtgccga
     1741 tagctgcggc tcctgcggcg aaggccgcgg cacccgccgc cgcagctccc gcccctcagg
     1801 cggcggcgcc tgcctcgcag accaacggcc atgcccgtat tttctcatcg ccgctggcgc
     1861 gacgcctggc gaaagaagcc ggcatcgagc tggcgcgcat caacggttcc ggcccgcatg
     1921 gccgcgtcat cgcgcgcgac gtcgaagaag ccagatcggg caaggggctg aaagccccgg
     1981 ctgctgcgcc cgccggcgcg ccgagcattg ccccatcgat gtcggacaag caggtccgcg
     2041 cgctttacga ggacggcagt tatgaggtga tcccgcatga cggcatgcgc cgcacgatcg
     2101 cgcaacgcct caccgcgtca gtccagacca ttccgcattt ctacctgacg atcgactgca
     2161 acatcggcaa gctggtcgag gcgcgcgagg agatcaatgc cgccgcaccc aaggacaagg
     2221 acggcaagcc ggcctacaag ctctcggtca acgatttcgt catcaaggcg ctggcgatcg
     2281 cgctgcagcg cattcccaat gccaatgtga gctggaccga cggcggcatg ctgaaacaca
     2341 agcattccga tatcggcgta gccgtggcga tgcccggcgg cctgatcacg ccgatcatcc
     2401 gcaaagccga gaccaagccg atctcgatca tctccgccga gatgaaggat ttcgctgccc
     2461 gcgcccgcgc ccgcaaactc aagccggagg aatatcaggg cggcaccacc gccgtctcca
     2521 atctcggcat gtacggcatc aaccacttta ccgcggtgat caatccgccg cacgccacca
     2581 tcctcgcggt cggcaccggc gaggagcggg caatcgtcaa gcacggcaag atcgaagtcg
     2641 cgcagatcat gagcgtgacg atgtcctgcg accatcgtgc agtcgacggc gcgctcggcg
     2701 ccgaactgat cggggcgttc aaattgctga tcgaaaatcc ggtcatgatg gtggtgtgag
     2761 ataacgagaa atgtcgaatg cggcccttct tccttcttcc ctctcccctt gtgggagagg
     2821 gtggccgcca ccaaagtcgg atatatccga ctttggcata atgagaggca aactcggcaa
     2881 cagccgagtt tgatggcgga cgggtgaggg ggcgagtgag ggggatgcgc caaagcgtac
     2941 cggggcagaa gcgagctctt gccaaagctc tccacgcgaa ggcgacaggt gccgagatca
     3001 aactctggcg actgcttcgt tcgcgtcgct ttgcaagtat gaagtttcgt cgccaagttc
     3061 caatcgggcc gtggatcgtg gattttgtct ccttcgaaca gcgtttgatt atcgaagcag
     3121 atggcagcca gcacgcggaa agcgaaaatg acaagcggcg cgattacgat ctgtcggagc
     3181 gcggatttcg tgttctgcgg ttctggaata acgacgttct gatgcggccg cagtcggtca
     3241 tggaaatgat tgtggatgtc gtcgcgcagt ccccctcacc cgtctgcgcg ccaagcggcg
     3301 cgcagccacc ctctcccaca aggggagagg gaagtaagta agcgagttcg caatgcccga
     3361 cacatccttc gacgtcatta ttatcggttc cgggcccggc ggctacgtca ccgcgatccg
     3421 cgcagcgcag cttggttata aaaccgcgat tgtggaaaaa tcctatctcg gcggcatttg
     3481 tctgaactgg ggctgcatcc cgaccaaggc gctgctgcgc tcggcggaaa tctttcacta
     3541 catgcagcat gcaaaggatt acgggctgtc ggctgacaac gtctcgttcg atccaaaggc
     3601 ggtggtgcag cgttcgcgcg gcgtggcaaa gcgcctcaac gacggcgtcg gctttctgat
     3661 gaagaagaac aaggtcacgg tgatttgggg tgaagcttca atcgacgcac ccggcaagat
     3721 cacggtcaag aaatccgccg tcgaggcacc aaagggcgcg tcaggggagg gtgcctacca
     3781 agcaaaacac attattctgg cgaccggcgc gcggccgcgg ttgctgccgg ggctcgagcc
     3841 cgaccagaaa ctgatctgga cctatttcga ggcgatggtg ccggagaaga tgccgaagtc
     3901 gctgctggtg gtcggctccg gcgcgatcgg catcgaattc gcgtcgttct tccacaccat
     3961 gggcgctgac gtgaccgtgg tcgaggtgct gccgcagata ttgccggtgg aagatgctga
     4021 aatcgccggc ctggcgcgaa aacggttcga gaagcagggc atcaagatcc tcaccaatac
     4081 caaggtgaca aaacttcaga aaaagaccga cagcgtgctc gccaccatcg acgacggcaa
     4141 gaagccgcaa acgatcgaat tcgatcgggt gatttcagcg gtcggcgtgg tcggcaatat
     4201 cgaaaatctc ggcttggaaa aactcggcgt caaaaccgaa cgcggcgttg tcgtcatcga
     4261 tggctacggc aagaccagcg tgcccggcat ctacgccatc ggcgacgttg cggggccgcc
     4321 gatgctggcg cacaaggccg agcatgaagg cgtggtgtgc atcgaggcca tcaagggtct
     4381 gcatccgcat gccacggaca agaatctcat tccgggctgc acctattgca atccgcaagt
     4441 agcctcggtc gggctcaccg aggcgaaggc aaaggagggc ggccgtgaga tccgtgtcgg
     4501 gcgttttccg tttgtcggca acggcaaggc gatcgcactg ggtgaggacc agggcctggt
     4561 caaggtggtc ttcgacaaga agaccggcca gttgctcggg gcgcatatga tcggtgcaga
     4621 agtgaccgaa ctgatccagg gctacgtggt tgccatgaac ctcgaaacca cggaagaaga
     4681 actgatgcac accgtgttcc cgcacccgac gctatcagag atgatgaagg aagcggtgct
     4741 ggatgcctac ggccgcgtgc tgaatatcta aacgcgtgct gaacatctaa aaaagagacc
     4801 ttatcccgtg aaagacaatg acaatctgac gatcgagcgt ccgacgttcg tgacccatct
     4861 cgaatgcgcg atggaaggcg atcactatcc cgccgaccag gtccataacc tctccaaggc
     4921 cggcaagccg ctgttggtgc gctacgacct tgccggggtg aagcaggctt tgaccaaaga
     4981 cgcgctggcg cagcggcccg ccgatctatg gcgttatcgc gagatgctgc cggtgcggaa
     5041 aatcgccgac atcgtcagcc tcggcgaagt gacgacgccg ctgatacgcc ttccgaagct
     5101 cgccgcaaaa ctcggcggcg gcgaaatcat cgtcaaagac gaaggccggt tgccgaccgg
     5161 ctcgttcaag gcgcgcgggc ttgtcatggc ggtgtcgatg ggcaaggcgc tccgcatcaa
     5221 gcacatggcg atgccgacca acggca
//
LOCUS       DASWGM010000847         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_110378,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000847 DASWGM010000000
VERSION     DASWGM010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110378"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VGA15_23165"
     CDS             complement(<1..471)
                     /locus_tag="VGA15_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590630.1"
                     /translation="MQLKDVAVLITGGGSGLGAATARAMAAKGAKIAVLDQSKENAEK
                     VAAEVKGVAVHADVTDEEQVKAGIAKAEAAHGVARVLMNCAGIGGSQRIVGKDGVYPL
                     AKFVRIINVNLIGTFNVLRLFAERLVTAEPIGEERGVIINTASVAAYEGQIGQIA"
     gene            complement(594..1286)
                     /locus_tag="VGA15_23170"
     CDS             complement(594..1286)
                     /locus_tag="VGA15_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX9590631.1"
                     /translation="MGSQLNTSVPNGLPLAKNTTAEKLLVAASELMIERSSIEVSLSD
                     IAQKSGVNAALVKYHFGNKDGLLLALLARDAATEVTQLEYLLAQPIAPTAKLKLHIAG
                     IIRAYHQFPYMNRLIHYLLHESSAEAADEVSKFFVAPLLDFHRRLLAEGVRCGEFRNI
                     DPVLFYTSLIGACDHLFFGRHAMSRATGVGPVTDEVCRQYIRHMEALICGGMLNERKE
                     RMASSEEMANRE"
     gene            1436..>2318
                     /locus_tag="VGA15_23175"
     CDS             1436..>2318
                     /locus_tag="VGA15_23175"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9590632.1"
                     /translation="MAEAYIVAAARTAGGRKGGRLAGWHPADLAAAVLDSLIERSGAA
                     PDQVEDVIMGCVMQTGEQSNNVARNAIMASRLPESVPGTSIDRQCGSSQQALHFAAQA
                     VMSGSMDCVIAAGVESMTRVPMGLASQLPAKNGFGTYKSPNIEKRYPNIVFSQFTGAE
                     MMAEKYGLSKDELDEYSYNSHQRAMAATQAGKFKDEIIPLQITRADGSTDTHTIDEGI
                     RFDASLEGIRNVKLIAENGKLTAASASQICDGASGVMVVNEKGLKALGVKPMARIHHM
                     TMMGGDPVIMLDAPLHAT"
BASE COUNT          456 a          725 c          697 g          440 t
ORIGIN      
        1 agcgatctgg ccgatctggc cttcataggc cgcgacgctc gcggtgttga tgatgacgcc
       61 gcgctcttcg ccgatcggct ctgcggtcac gaggcgctcg gcgaacagcc gcagcacgtt
      121 gaaggtgccg atcagattga cgttgatgat gcggacaaac ttggccagcg gatagacacc
      181 gtccttgccg acgatgcgct gcgatccgcc gatgccggcg cagttcatca gcacccgcgc
      241 gacgccatgc gcagcctcgg ctttcgcgat tcccgccttg acctgctcct cgtcggtgac
      301 gtcggcatgg acggcgacgc ctttcacttc ggcggcgacc ttctcggcat tctccttgct
      361 ctggtctagc acggcgatct tggcgccttt ggcggccatg gcgcgcgcgg tggcggcgcc
      421 gagaccggag ccaccgccgg tgatgagaac ggcaacgtct ttcaactgca tgatagaaac
      481 ctttccttgg gcgtttcttc ttgagcggcg aatagggagt agcgaatagc gaatggaaga
      541 aaaagcagac acctctacct gtgtcctatt ttcttgcccc tattcgctat tcgctactcc
      601 ctattcgcca tttcttcgct actcgccatt cgctccttcc tttcgttcag catgccaccg
      661 cagatcagcg cttccatatg cctgatgtat tggcggcaga cttcgtcggt gaccggcccg
      721 acgccggtgg cgcgcgacat cgcgtgccgc ccgaaaaaca ggtggtcgca ggcgccgatc
      781 aggctggtgt agaacagcac cggatcgatg ttgcggaact cgccgcacct gacgccttcc
      841 gccagcagcc ggcggtgaaa gtccaacagc ggcgcgacaa agaattttga aacctcgtcg
      901 gcggcctccg cgctgctttc atgcaggaga tagtggatca gccggttcat gtaggggaac
      961 tggtgatagg cccggatgat gccggcgata tgcagcttga gtttcgcggt cggcgcgatc
     1021 ggctgcgcca gcagatattc gagctgcgtc acctcggtgg cggcatcccg cgccaaaagc
     1081 gccagcagca agccgtcctt gttgccaaag tgatatttca ccagcgcggc gttgacgccg
     1141 gatttttgcg caatgtcgct cagggatacc tcgatcgaag aacgctcgat catcagctcg
     1201 ctggccgcga caagcagctt ctcggcggtg gtgtttttcg ccaacggaag cccgttcggt
     1261 acgctggtat tcaactggga tcccatgacc tgtccgccgg ggaaatacat gcgcagataa
     1321 cccgatggca gcgccgtact caagagttaa ttgattgatt gactaaatcc gccggcatca
     1381 cttaaatcgg cacccgttgt tcaagcacca tccttaacaa tcagggagaa actacatggc
     1441 cgaggcttat atcgtcgccg ccgcacgcac cgcaggcggc cgcaagggag gacgcctcgc
     1501 gggctggcat ccggccgatc tcgccgctgc cgtgctggat tcgctgattg agcgctcggg
     1561 cgcggcgccc gatcaggtcg aagacgtgat catgggctgc gtgatgcaga ccggcgaaca
     1621 gtccaacaac gtcgcgcgca acgccatcat ggcttcaaga ctgccggaaa gcgtgcccgg
     1681 cacctcgatc gaccgccagt gcggttcttc gcagcaggcg ctgcactttg ccgcgcaagc
     1741 cgtgatgtcc ggcagcatgg actgcgtcat cgccgccggc gtcgaatcga tgacgcgggt
     1801 gccgatgggc ctcgcgtcgc aattgccggc caagaacggg tttggcacct acaagagccc
     1861 gaacatcgag aagcgctatc cgaacatcgt gttcagccag ttcaccggcg ccgagatgat
     1921 ggccgagaag tacggcctct cgaaggatga gctcgacgaa tattcctaca acagccatca
     1981 gcgtgccatg gccgccacgc aagcgggaaa attcaaggac gagatcatcc cgctgcagat
     2041 cacccgcgcc gatggatcga ccgataccca cacaatcgat gaaggcatcc gtttcgacgc
     2101 cagcctcgag ggcatccgca acgtcaagct gatcgcggag aacggcaagc tgaccgccgc
     2161 cagcgccagc cagatctgcg acggcgcatc gggcgtgatg gtcgtcaatg agaagggcct
     2221 gaaggcgctc ggggtcaagc cgatggcccg gatccaccac atgaccatga tgggcggcga
     2281 tcccgtgatc atgctggatg cgccgctgca tgccaccg
//
LOCUS       DASWGM010000848         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_504189,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000848 DASWGM010000000
VERSION     DASWGM010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504189"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="dctP"
                     /locus_tag="VGA15_23180"
     CDS             complement(<1..582)
                     /gene="dctP"
                     /locus_tag="VGA15_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX9590633.1"
                     /translation="MPVYSRADLSRTIAPVVALFLAAVWTSAVAREFRAADSQNEDYP
                     TVQALRYMGRMVAERSGGRHQIRVFHSRQLGEEKETIEQTRAGAIDLNRTNGALIGNF
                     VPAMNVLAMPFLFRSMEHLRKVLDGPIGNEILNSFQPYGFVGLAFYDSGARSIYNSVR
                     PVRSIADIKGLRLRVQQSELMSDTIKALGAEPIE"
     gene            793..2118
                     /gene="ugpB"
                     /locus_tag="VGA15_23185"
     CDS             793..2118
                     /gene="ugpB"
                     /locus_tag="VGA15_23185"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEX9590634.1"
                     /translation="MKSTLRFVQLAVVAAAVTLASPALAVTDIMWWHAMSGELGRQLD
                     KLATDFNASQSEYRIVPSYKGNYTETVTAAIFAFRSRSQPAIVQVSEIATATMMAARG
                     AIYPVFELMRDEQEAFSTAAYLPAITGYYTDVAGNMLSFPFNSSTPILYYNKNLFRAA
                     GLDPEVAPKTWGEVGAAAKRLRAYGAVCGFTTSWPSWINVENFSAFHNLPMSTKANGF
                     DGLDAVLTFNNPLMVRHVAQLAEWQATKMFDYSGRATSAEPRFQNGECGIFIGSSATR
                     ADIRANSKFEVGYGMLPYWPDVEGAPQNTTIGGATLWVLRDRPRDEYKGVAKFFGYLS
                     KPEIQAAWHQNTGYLPITRAAFELTRAQGFYDRNPGTAISIEQITLKAPTLNSRGVRL
                     GSFVLIRDVIDDELEQAFSGKKSAQAALDSAVERGNRLLRQFERANPDR"
     gene            2414..>2758
                     /locus_tag="VGA15_23190"
     CDS             2414..>2758
                     /locus_tag="VGA15_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX9590635.1"
                     /translation="MKRRDFLKSVSGIAAGAMVPAPAIWSSARADARSETLLVVTEGG
                     PNNLDIHGVGTNRPGYEASWNCYDRLISHETKTLPDGTLSYDRDKFKPELAEDMSIGD
                     MSVTFKLKKKAKF"
BASE COUNT          570 a          840 c          806 g          542 t
ORIGIN      
        1 ctcgatcggc tccgcgccga gtgccttgat cgtgtcggac atcaattcgg attgttgcac
       61 gcggagccgc aagcccttga tgtcggcgat cgagcgcacc ggcctgacgc tgttgtagat
      121 cgatcgcgcc ccggaatcgt agaacgccag gccgacaaag ccatagggtt ggaaactgtt
      181 caggatttcg ttgccgatcg gcccgtccag cactttccgt aaatgctcca tggagcgaaa
      241 cagaaacggc atcgccagca cgttcatcgc cggcacgaaa ttcccgatca gcgccccgtt
      301 ggtgcgattg agatcgatgg cgccggcgcg ggtctgttcg atggtttcct tttcctcgcc
      361 gagctgccgc gaatggaaca cccggatctg gtggcggccg ccgctgcgct ccgcgaccat
      421 gcggcccata tagcgcagtg cctggacggt cggataatcc tcgttctggc tgtcggcggc
      481 gcgaaattcc cgtgcgacag cgctcgtcca gaccgccgcc aaaaacagcg cgacgacagg
      541 tgcgatggtc cgcgaaagat cggcgcggct gtacacgggc aactgcatcc ccttatgatg
      601 gtgtcggcta agggaagaaa aggttcaacg cgattttata gaagcctgcg aggtatagct
      661 atactgtcga tgcggctatc gttaatggtg caccgcgacc gcccttgcag attgagaccg
      721 cgggtgctgg cttaatgagg gcgcgtgacg cgcggaaacc gtttcgaccg cggcagatcg
      781 gaaggtctta tcgtgaaatc gaccttgagg ttcgtgcaac tcgcggttgt cgccgcggcg
      841 gtcactttgg cctctccggc tctcgctgtc accgacatca tgtggtggca tgccatgtcg
      901 ggcgaactcg ggcggcagtt ggataagctc gccaccgatt tcaacgcatc gcaatccgaa
      961 taccggatcg tgccgagtta caaaggcaat tacaccgaaa ccgtaacggc ggcgatcttc
     1021 gcgtttcgtt cgcggagcca gcccgccatc gtgcaggtca gcgagatagc gaccgccacc
     1081 atgatggctg cgcgaggcgc gatctatccc gtgttcgaat tgatgcgcga cgaacaggag
     1141 gcattttcca cggcggcgta ccttccggcc attaccggct attacaccga cgtcgccggc
     1201 aacatgctgt cattcccgtt caactcctcg acgccgatcc tgtattataa caagaacctg
     1261 tttcgcgccg ccggcctcga ccccgaagtg gcgccgaaga cctggggcga agtcggcgcg
     1321 gcggcgaagc ggctgcgtgc ttacggtgcc gtttgcggtt tcacgacgtc ctggccttca
     1381 tggatcaacg tcgagaattt ttcggcgttc cacaatctgc cgatgtcgac caaagccaac
     1441 ggctttgacg gcctcgatgc ggtgctgact ttcaacaatc cgctgatggt gcgccacgtc
     1501 gcgcaactcg cggagtggca ggccaccaag atgttcgact acagcggccg cgccacctcg
     1561 gcggagccgc gatttcaaaa tggcgaatgc gggatcttca tcggctcatc ggcgacacgc
     1621 gcggatatca gggccaattc gaaattcgag gtcggatacg gcatgttgcc gtattggccc
     1681 gatgttgaag gcgcgccgca aaacaccacg atcggcggcg cgacattgtg ggtgctgcgg
     1741 gaccggccgc gcgacgaata caagggcgtc gcaaaattct tcggatacct gtcgaagccg
     1801 gagattcagg cggcatggca ccagaacacc ggctatctgc cgattacccg ggcggctttc
     1861 gagttgaccc gcgcccaggg tttctacgat cgcaatcccg gcacggcgat ctcgatcgag
     1921 cagattaccc tgaaagcacc gaccctaaat tcgaggggag ttcggcttgg atccttcgtt
     1981 ctgattcgcg atgtgatcga cgacgaactg gagcaggcct tcagcggcaa aaaatccgcg
     2041 caagccgcac tggattcggc cgtcgaacgc ggcaaccggc tgctgcgtca attcgagcgg
     2101 gccaacccgg accggtaagc tgcggccagc ttcgtcgtga agcgcgcgat gcgcgtcggg
     2161 caatccgaat ttccatcacc gcagccactt cgcccaggat aaagggcgcg ccgaaagatt
     2221 tgcaccgata ataagcaaat atgtttcttt gtatgcaatg aaccttctgg cacgtgatat
     2281 cgcggtcccc tccgaaaact caatattgaa gccaaatcat gcgcttaaag cacgcttgag
     2341 cttccgttaa ggtttggcac aaaccttgcg atgcccctcc aaagccgttt cccgccggcg
     2401 tttggagcat cacatgaagc gtcgcgattt tctgaaatcc gtctccggca ttgccgccgg
     2461 cgcgatggtg ccggcgccgg cgatctggtc ctccgccagg gccgacgccc gctctgaaac
     2521 gcttctcgtc gtcaccgaag gcggccccaa caatctcgac atccacggcg tcggcaccaa
     2581 tcggcccggc tacgaggcgt cgtggaattg ctacgatcgc ctgatcagcc atgaaaccaa
     2641 gacactgccg gatggcacgc tgtcttacga tcgcgacaag ttcaaacccg aactcgctga
     2701 agacatgagc atcggcgaca tgtccgtcac cttcaagctg aagaaaaaag ccaaattc
//
LOCUS       DASWGM010000849         2761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2183765,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000849 DASWGM010000000
VERSION     DASWGM010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183765"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /locus_tag="VGA15_23195"
     CDS             <1..2047
                     /locus_tag="VGA15_23195"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX9590636.1"
                     /translation="DIAPFVWRKHLDFAALADVHDMKRQMQTFRGQSEISVEGHNVKI
                     GRGGIREIEFFAQTQQLIAGGRHPQLRVRPTLEALNVLASSHWITFEARDQLTAAYEF
                     LRRVEHRLQMVADEQTHTLPDDVEAVERFAAFFGYENRAAFAKDLLGHLNVVQAHYGK
                     LFEGDPTGNVQLPAADYSAGPDDPRLLEHLATLGFKKPIMVAQTVQQWMVGDYRVFRL
                     ESTRNAFVEFVPALIAGLANAEEPDHAVTAFDRLLQALQRGGRLISLLSQNRDLVALV
                     ALILGAAPRLGDMLARQPQIMDGLIDPRFFGAMPDRSELSARLAATLTDANSYEEFLD
                     RLRLFGQESLFLIGTRILSGTVSAQQASVAFADVAEGIVHTVHGLVTDRFAAQHGRIK
                     RQETAILAMGRLGSREMTASSDLDLILLYDFDSEHPDSDGAKSLHGAHYFARFTQRLI
                     SAFTTRTNYGVLYDVDMRLRPSGRAGPVASRFDAFADYQEREAWTWEHMALTRARVIS
                     SSPDFRGKIERIIREVLTRPRNAAATAVDVADMRRAIALEKGERDVWDLKYAAGGMVD
                     IDFIAQYLQLVHAADKPDILDVSTLHVLDNAARLGVLPQSEAEVLRSAARLYHDLTQI
                     LRLCVTERFKPETSGEDLLRVMARAGDAPDFSSLEARVKETQTEVRRVFRFVVEGRC"
BASE COUNT          490 a          867 c          882 g          522 t
ORIGIN      
        1 ggacatcgcg ccgttcgtct ggcgcaagca tctggatttt gccgcccttg ccgacgtgca
       61 cgacatgaag cggcagatgc agacctttcg cggccaaagc gagatctcgg tggaaggcca
      121 caatgtcaag atcggccgcg gtggcatccg cgaaatcgag tttttcgcgc agacccagca
      181 gttgatcgcc ggcggccggc atccgcaatt gcgggtgcgc ccgacgctgg aagccttgaa
      241 tgtgctggcg tcgagccact ggatcacctt cgaggcacgc gaccagctga ccgcggccta
      301 tgagtttctg cggcgggtcg aacaccggct gcagatggtg gcggacgagc agacccatac
      361 cctgcctgac gatgtcgagg cggtagagcg ctttgccgct ttctttggct acgagaaccg
      421 cgcggcgttc gcaaaggatc tgctcggcca tctcaatgtc gtgcaggcgc attacggcaa
      481 gctgttcgaa ggcgatccga ccggcaatgt acaactgccg gcggcggatt acagcgcggg
      541 ccccgacgat ccgcgcctgc tcgagcatct ggccacgctc ggcttcaaga agccaatcat
      601 ggtggcgcag accgtgcagc aatggatggt cggcgactat cgcgtgttcc ggctggaatc
      661 gacgcggaat gcgtttgtcg aattcgttcc cgcattgatc gccggcctgg cgaacgccga
      721 agaacccgac catgcggtga cagccttcga ccgcctcctg caggcgctgc agcggggagg
      781 gcggctgatt tcgctgctca gccagaaccg cgacctcgtc gcattggtgg cgctgattct
      841 cggcgccgca cctcgcctcg gcgacatgct ggcgcggcaa ccgcagatca tggacggttt
      901 gatcgatccg cgcttcttcg gcgcgatgcc ggaccgcagc gaattgtccg cgcggctcgc
      961 ggcgaccctg acggacgcga attcctacga ggagtttctc gaccgattgc ggctgttcgg
     1021 ccaggaaagc ctgttcctga tcggcacgcg aatcctgtcg ggcacggtct cggcccagca
     1081 ggccagcgtc gcctttgccg atgtcgccga gggcatcgtc cataccgtgc acggcttggt
     1141 caccgaccgg ttcgcggccc agcacggccg gatcaagcga caggagaccg cgatcctggc
     1201 gatgggccgg cttggcagcc gcgagatgac cgcttcgtcc gatctcgatc tgatcctgct
     1261 ctatgatttc gattccgaac atccggactc cgacggcgcg aaatcgctgc acggcgcgca
     1321 ttatttcgca cgcttcaccc agcggttgat cagcgcattc acgacgcgga cgaattacgg
     1381 cgtactctac gacgtggata tgcggctgcg cccgtcgggg cgcgcggggc cggtggcttc
     1441 gcggttcgac gccttcgccg attaccagga gcgggaagcc tggacctggg agcacatggc
     1501 gctgacccgc gcccgggtga tttcttcgtc gcctgacttt cgcggcaaga tcgagcggat
     1561 catccgcgag gtgctgacgc gaccgcgcaa cgccgcggcc acagccgtcg acgtcgcgga
     1621 catgcgccgc gccatcgcgc tggagaaggg cgagcgcgac gtctgggacc tgaaatatgc
     1681 cgcgggcggc atggtcgata tcgatttcat tgcccaatat ctccagttgg ttcacgccgc
     1741 cgacaagccg gatattctcg acgtctctac gctgcacgtg ctcgacaacg ccgcgcggct
     1801 cggcgtcctg ccgcaatcgg aagcggaggt cttgcgttcg gcagcacgac tttatcatga
     1861 cctcacgcaa atcctgcggc tctgcgtcac cgagagattc aaaccggaaa catcgggcga
     1921 ggatctcttg cgggtgatgg cgcgggccgg cgacgcaccg gatttttcgt cgctggaagc
     1981 gcgggtgaag gaaacccaga ccgaagtacg ccgggtgttt cggttcgtag tcgaaggccg
     2041 ctgctagctc gcgcaaagcg ctgatacaat tttcgaacag ccaggtcgtc attgcgagcc
     2101 accgggtcgc gcgaatgcgc gcccgatgac aggctccgcg aagcaatcca tgcttgccgc
     2161 gaagaaagta tggattgctt cgtcgcggag tttatcatcg ggccggccga aggccggact
     2221 cgcaatgacg tgacggactt gggtgccgcg ctgctccaaa acgagtcgtc ataccccgcg
     2281 aaaggggtat ccagtacgct gcggcctctc gattccatca ttgatgtctc ggaataatgg
     2341 atcgcccgcc ggagcctgtc atcgggcggc cattcggccg acccggtggc gggcgacgac
     2401 ggctgagagt gtggcgtact catactcatc agatacggct tcgcgatctc acggcgcgat
     2461 gcgcccgagc tgtgcattta tcttccgccc cctggagggc gtggggaatg ccgggtgccc
     2521 gctgcacccg cggcctcgtg tgcactttgt attggtagaa cgcacacgag taacaacgag
     2581 tacaccagaa cgcccggcat tcccgcacgc aatggtttta acggcttatg tcgtgctctc
     2641 cccggtgacc gggctctttt gccaccgtcg tcagcggata ggttttgtct aagcccggtt
     2701 gggccgacgc aactccgcgt acttgacgcc agcgtcgggg cgtcaggacc acacgatttt
     2761 g
//
LOCUS       DASWGM010000850         6865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_504399,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000850 DASWGM010000000
VERSION     DASWGM010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504399"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..976
                     /locus_tag="VGA15_23200"
     CDS             101..976
                     /locus_tag="VGA15_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590637.1"
                     /translation="MDLVLQLLFTGIGIGAVYALVALGFVLIFRATNVVNFAQGEFSM
                     VAAYLMVVFAVDLHLPYWLSFLLALAGMALLGAIFNLGVYYPLRHRTFLPVIIATIGA
                     SILMANSVLAIYGPQPQVLEGWFDTPGIQLGPVYLDSQYLLIIAVTMILVVFNYWFFE
                     HTMLGKKLQATSQDKEMASLLGISVSTMIMITFIYSAVLGGLAGILVAPVLFVSIQMG
                     STIALKAFAATIIGGFGDVAGAIIGGLALGIIETFGAAYVSVPYKDGFAFLVLVLFLV
                     FRPQGIFGERVAEKA"
     gene            973..2757
                     /locus_tag="VGA15_23205"
     CDS             973..2757
                     /locus_tag="VGA15_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9590638.1"
                     /translation="MSAPSENMPMAVSTTARAKPLLLRHLPYFIGAAVLVTLAATMRF
                     DGYIHNILMQATTFAIAVFGLSVVLGLCGQINLAQAAFFGLGAYAVGLGTSDYHLSYW
                     LCLAAGCVIALLAGALLGASTLRLGGHYLAMVTISFQQIVTLVMINTIWLTHGPDGVA
                     RIGRPDLFQSSQSYLAFCVAMLALVGYFVWHLSDTRLGRAMRAVRDNELAAGVVGIDV
                     FRTKVSAFALSAVLGGLAGGLFAGGFAYVSPDQFSFAESVVFLTMSLLGGVASPIGSA
                     IGTGLLILIPEWLRFLKSVPGLYLAIYGLSVILIIRYMPDGIWGFVNLATARWRAKAK
                     VHASGPALQLAPASVGGGMVLEVKGLAKYFGGLKAVDDVDIEVRRGGVHALIGPNGSG
                     KTTTLNVLSGLYKATAGRVILDGTDVTDMPPHQRAAAGLGRTFQNIRLFRSMTALENV
                     VIGAERPGNTLLGQSGADPLTERAMSALTFVGLGSRANELISSFSYGHQRLIEIARAL
                     AANPTLLLLDEPAAGLNSTEKLELHELLKRIAAQGLTILIIDHDMTLVSEAAQHITVL
                     NFGRRIADGESMAVLRHPDVVSAYLGTE"
     gene            2757..3485
                     /locus_tag="VGA15_23210"
     CDS             2757..3485
                     /locus_tag="VGA15_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590639.1"
                     /translation="MPLLEINDLIVLYGEIEALRGVTISVDEGQVVTLLGANGAGKST
                     TLRAISGLAKPAAGEILFDGKSIAGLGPETIVRLGISHVPEGRRVFPGLTVKENIMLG
                     ASNRRVAKSELSREADAMFDLFPDIRSFSNALGWTLSGGQLQMVAVARGLMAKPRLLL
                     LDEPSLGLAPVIVQAVFRIISEIRRNTTVLLVEQNARMGLSVADHGYVLETGRIVLGG
                     KPDELWGNEAIAAAYLGGHAKVSA"
     gene            3604..4689
                     /locus_tag="VGA15_23215"
     CDS             3604..4689
                     /locus_tag="VGA15_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEX9590640.1"
                     /translation="MVTIKVQNLINFVAEVFAHSESSAEEARRIATYLTTANLTGHDS
                     HGVIRVPVYIRWKKMGSVVPDQTVELVVDTPSLAVVDGKFGYGQTVAPQAVKIGIEKC
                     KTAGLAAVALRNAGHIGRVGDWAEMAAAEGLVSIHFVNAAGSALVAPYGGVERRLSTA
                     PYCVGIPRKGQGPIVLDFATSIVAEGKVLVASRGGKKLPNGALIDLDGTLSEDPAVLY
                     GPHTKEGPRDHSQGKGAIRAFGEHKGSGLALICELLGGALTGTGATGPNRRFANGMFA
                     VYVDPKVVDTSNVFDGEISRYVTYFKDTKPAAGIDAVLIPGEPEAAMRADRTKNGVPL
                     PDETWAAIVNTAREAGVSEVSIQRATA"
     gene            4794..5570
                     /locus_tag="VGA15_23220"
     CDS             4794..5570
                     /locus_tag="VGA15_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX9590641.1"
                     /translation="MANNVKKVWASGKTVVNAWLAIPSGFSAEVIAQCGFDSVTVDMQ
                     HGVQDYLSMVQCFQAMNGHPVTPMVRVPWNEPGIIGKVLDGGAYGVICPMINTKQEAE
                     NLVVYSKYPPRGTRSNGPIRSGMYGSAGTYQQTANDEIVLLPMMETKTAVENMESILD
                     VEGINGVYIGPSDLGFSYGLVPKLDRDEPEILKIYEKIVKECGKRGLYPGIHCSGADG
                     ALRAINMGFKLVTLSNESGLMMTYAKMQVAQTRKDSGGKA"
     gene            5745..>6865
                     /locus_tag="VGA15_23225"
     CDS             5745..>6865
                     /locus_tag="VGA15_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX9590642.1"
                     /translation="MTSAPVIRLHPDDGVLIARSSLPPGMVVADGVTTVERIPAGHKV
                     ATRAIAVGEPIRRYGQIIGFATAPIAPGQHVHSQNCGMGDFAKDYAFGVDVTPTPNFD
                     LPATFDGIRRPDGRVATRNYIGILTSVNCSAHVAGMVADIFKKNPFTGDNPLVDFPNV
                     DGVVALTHKTGCGMTQDEPLTILRRTLGGYARHVNFSAVVVLGLGCEVNQIGGLMKEQ
                     KLAGRLRELEIQEIGGTRKTVEAGVAFVREALTDANNVKREPVSASELTVALQCGGSD
                     GYSGVSANPALGAASDLLVRHGGTVILSETPETYGAEHLLTRRAVSREVGEKLVALMR
                     WWEEYTAREGAEMNANPSPGNKAGGLTTILEKSLGAMAKA"
BASE COUNT         1225 a         2102 c         2195 g         1343 t
ORIGIN      
        1 ccttgcgttg cgctgatcga tccaccccga ttttggcctg cggcaacagc aacaatcacc
       61 aacagcagag ccgatcagct tccagtaacc gagcgtttcc atggatctcg tactgcaatt
      121 gctcttcacc ggtataggca ttggcgcggt ctatgcgctg gtcgcgctcg gcttcgtgct
      181 gatcttccgc gccaccaacg tggtcaattt cgcgcaaggc gaattctcca tggtcgcggc
      241 ctatctgatg gtggtgttcg ccgtcgattt gcacttgccg tactggctgt cgttcctgct
      301 ggcgctggcc ggcatggcgc tattaggcgc gattttcaat ctcggggtct attatccgct
      361 gcgtcaccgc acgttccttc cggtgatcat cgcgaccatc ggcgcatcga ttttaatggc
      421 caattcggtg ctggcgatct acggcccgca gccgcaggtg ctggagggat ggttcgatac
      481 acccggcatc cagctcgggc cggtctatct cgacagtcag tatctcctga tcatcgcggt
      541 cacgatgatc ctggtagtgt tcaattactg gttcttcgag cacaccatgc tcggcaagaa
      601 gctgcaggcg acatcgcagg acaaggaaat ggcctcgctg ctcggcatct ccgtgtcgac
      661 catgatcatg atcaccttca tctattcggc agtgctcggc ggtctcgccg gcattctggt
      721 ggcgccggtc ctgtttgtct cgatccagat gggctcgacc atcgcgctca aggcctttgc
      781 cgccaccatc atcggcggct tcggcgacgt cgccggcgcg atcatcggcg ggctggcgct
      841 cggcatcatc gaaacgtttg gcgcggctta tgtgtcggtg ccctacaagg acggctttgc
      901 cttcctcgtg ctggtgctgt ttctggtgtt ccggcctcag ggcatcttcg gcgaacgcgt
      961 cgcggagaaa gcatgagcgc ccccagcgag aacatgccga tggccgtgtc caccaccgcg
     1021 cgcgcaaaac cgctgctgtt gcggcatctg ccgtatttca tcggcgccgc tgtcctggtg
     1081 acgctcgccg ccacgatgcg gttcgacggc tacatccaca acatcctgat gcaggcgacc
     1141 acctttgcga tcgcggtgtt cggtctgtcg gtggtgctcg gcctgtgcgg ccagatcaat
     1201 ctggcgcagg cggcgttctt cggcttgggc gcttacgccg tcgggctcgg aacgtcggac
     1261 tatcatctca gctattggct ttgtcttgcg gccggttgcg tcatcgcgct attggctggt
     1321 gcgttgctgg gcgcctcgac gctgcggctt ggcgggcatt atctggcgat ggtgacgatt
     1381 tcgtttcagc agatcgtcac gctggtcatg atcaacacca tctggctcac ccacggcccg
     1441 gacggtgtgg cgcggatcgg ccggcccgat ctgttccagt cctcgcagtc ctatttggcc
     1501 ttctgcgtcg ccatgctggc gctggttggt tatttcgtct ggcatctgtc ggatacgcgg
     1561 ctcgggcgcg cgatgcgcgc ggtgcgcgat aacgagcttg cggccggcgt cgtcggcatc
     1621 gacgtctttc gcaccaaggt ttccgccttt gcgctgagcg cggtgctggg cggattggcc
     1681 ggcggtctgt tcgcaggcgg ttttgcctat gtcagcccgg atcagttctc gttcgccgag
     1741 tcggtcgtgt tcctcaccat gtccctgctt ggcggcgtcg cgtcgccgat agggtcggcg
     1801 atcggaacag ggctactgat cctgattccg gaatggctgc ggtttctcaa aagcgttccc
     1861 gggctgtatc tggcgatcta cggattgtcc gtcatcctga tcatccgcta catgcccgac
     1921 ggcatctggg gattcgtcaa ccttgcgacc gcgcgctggc gcgccaaggc caaggtgcat
     1981 gcctcgggtc cggcgctgca attggctccg gcgtcggttg gcggcggcat ggttctggag
     2041 gtcaaaggcc tcgccaaata tttcggcggc ctcaaggccg tcgatgatgt cgatatcgag
     2101 gtcaggcgtg gcggcgtcca cgcgctgatc gggccgaacg gctccggcaa gaccacgacg
     2161 ctgaatgtgc tttccggtct ctacaaggcg accgcgggca gggtgatcct cgacggcacc
     2221 gatgtcaccg atatgccgcc gcatcagcgc gccgccgcgg ggctggggcg aacgtttcag
     2281 aacatccggc tgttccgctc gatgacggcg ctggaaaacg tcgtgatcgg cgccgagcgg
     2341 ccgggcaata cgctactcgg acaaagcggc gctgatccgc tgaccgagcg cgcgatgtcg
     2401 gcgctgacct tcgtcgggct cggcagccgc gccaacgaac tgatctcgag cttttcctat
     2461 gggcatcagc gcctgatcga gatcgcgcgc gcgctcgccg ccaatccgac cttgctgctg
     2521 ctcgacgagc ccgcggccgg tttgaactcg accgaaaaac tcgaactgca tgagttgctc
     2581 aagcgcatcg ccgcccaagg cctgaccatc ctgatcatcg atcacgacat gaccctggtc
     2641 tccgaggcgg cccagcatat caccgtgctc aatttcggac gccgcatcgc ggacggcgaa
     2701 tccatggcgg tgctgcgcca tcccgatgtc gtctccgcct atctcgggac cgaataatgc
     2761 cgctgctcga aatcaacgac ctgattgtgc tctatggcga gatcgaagcg ctgcgcggcg
     2821 taacgatcag tgtcgatgag gggcaggtgg tgaccttgct cggcgccaac ggcgccggca
     2881 aatcgaccac gctgcgggcg atctccggcc tcgcaaaacc ggcggcgggc gaaatcctgt
     2941 tcgacggcaa atcgatcgcg gggcttggtc ctgaaaccat tgtccggctc ggcatctcgc
     3001 atgtgccgga ggggcggcgg gtgtttcccg gcctcacggt gaaggaaaac atcatgctcg
     3061 gcgcgtcgaa tcgccgggtt gcgaaatccg aattgtcgcg cgaagccgac gccatgttcg
     3121 atctgtttcc ggacatccgg tcgttttcca acgcgctcgg ctggaccttg tccggcgggc
     3181 aattgcagat ggtcgcagtg gcgcgggggc tgatggcgaa gccgcggctg ttgctgttgg
     3241 acgagccctc gctcgggctt gcgcccgtga tcgtgcaggc ggtgttccgc atcatttcgg
     3301 agatcaggcg caacaccacg gtcttgcttg tcgagcaaaa cgcgcgcatg ggactttccg
     3361 tcgccgatca cggctacgtg ctggaaaccg gccgcatcgt gctgggcggc aagcccgacg
     3421 agttgtgggg caatgaagcc atcgccgccg cctatctcgg cggtcatgcc aaagtcagtg
     3481 cttaagagca tgatcccgaa aagtgggtac cggttttcgg atgagatcat gctcaaacaa
     3541 aatgataaac gaagggtctg atccagcgga gctggagcag accctgaaga gagcggcgaa
     3601 tccatggtca ccataaaagt tcagaatctg atcaacttcg ttgcggaagt tttcgctcat
     3661 tcagagtctt cggccgaaga agccaggcgc atcgcgacat acctgacgac cgcgaatctc
     3721 acggggcacg acagccacgg cgtgatccgg gtgccggtct atatcaggtg gaagaagatg
     3781 ggctcggtcg tccccgacca gaccgtcgag ctcgtggtcg atacgccgtc gctggcggtg
     3841 gtcgacggca agtttggtta cgggcagacc gtcgcacccc aggctgtcaa gatcggcatc
     3901 gagaaatgca agacggcggg ccttgccgcg gtcgcgctgc gcaatgccgg acacatcggc
     3961 cgggtcggcg actgggccga aatggcggcg gccgaagggc tggtgtcgat ccattttgtc
     4021 aatgccgcag gctcagcgct ggtggctcct tacggcggcg tcgaacggcg gctgtcgacc
     4081 gcgccctatt gcgtcggtat tccgcggaag gggcagggac cgatcgtgct cgatttcgcg
     4141 acctcgatcg tcgccgaagg caaggtgctg gtggcgagcc gcggcggcaa gaagctgccg
     4201 aacggcgcgt tgatcgatct cgacggcacc ttgagcgaag acccggcggt gctttatgga
     4261 ccgcacacca aggaaggtcc gcgcgaccac agccagggca agggcgcgat ccgcgcgttc
     4321 ggcgagcaca aaggctcagg cctggcgttg atctgcgagt tgctcggcgg cgcactgacc
     4381 ggcaccggcg ccaccgggcc gaaccggcgc tttgccaacg gcatgttcgc tgtttatgtc
     4441 gatcccaagg tggtcgatac cagcaacgtc ttcgatggcg agatttcgcg ctatgtcacc
     4501 tacttcaagg acaccaagcc ggccgccggc atcgatgccg tgctgatccc gggcgagccg
     4561 gaagccgcca tgcgcgccga tcgtaccaaa aacggcgtgc ccttgccgga tgagacctgg
     4621 gcggcgatag tgaataccgc acgcgaagct ggcgtcagcg aggtgagcat acaaagggcg
     4681 acggcatgat tttgtaccgt cattgcgagc caacgggcga agcaatccat agctgcaagc
     4741 aaagagtgga ttgcgcaatg acgatcggtt ggaaaaaacg ggaggaataa atagtggcaa
     4801 ataacgtcaa gaaagtatgg gcctcgggca agaccgtggt gaacgcctgg cttgcgattc
     4861 cgtcaggatt ttccgccgag gtgattgcgc aatgcgggtt cgacagcgtc accgtcgaca
     4921 tgcagcacgg cgtgcaggat tacctgtcga tggtgcaatg ttttcaggcg atgaacgggc
     4981 atccggtgac gccgatggtg cgggttccct ggaacgagcc cggcatcatc ggcaaagtgt
     5041 tggacggcgg cgcctatggc gtgatctgcc cgatgatcaa caccaagcag gaagcggaga
     5101 acctggttgt ctacagtaaa tacccgccgc gcggcacgcg ctcgaacggc ccgatccgtt
     5161 ccggcatgta tggttcggcc ggcacctatc agcagaccgc caacgacgag atcgtgttgc
     5221 tcccgatgat ggagaccaag accgccgtcg agaacatgga atcgatcctc gacgtcgagg
     5281 gcatcaacgg cgtctatatc ggcccgtccg atctcggctt ctcctacggc ctggttccaa
     5341 aactcgatcg cgacgagccg gaaattctca agatttacga gaagatcgtc aaggagtgtg
     5401 gcaagcgcgg cctctatccc ggcatccatt gcagcggcgc cgacggcgcg ttgcgcgcga
     5461 tcaacatggg cttcaagctg gtgacgctgt ccaacgagag cggtctgatg atgacctacg
     5521 ccaagatgca ggtcgcgcag acccgcaagg attcaggcgg caaagcgtga agtatctgct
     5581 ccctcgcccc gcacttgcgg ggagagggtt ggggtgaggg gctctttccg cgaattctgt
     5641 gttagcgttt gacattgtgc ctcgcccctc acccggactg cttcgcagtc cgacctctcc
     5701 ccgtaaagga cggggagagg tgaagcgatc agaggagacc ccccatgacc tcagcccccg
     5761 tcattcgcct gcatcccgat gatggcgtgc tgatcgcgcg ctcgagcctg ccgcccggca
     5821 tggtggtggc tgacggcgtg accacggtcg aacgcattcc ggcgggccac aaggtcgcga
     5881 cccgcgccat cgcggtcggc gaaccgatcc gccgctacgg ccagatcatc ggctttgcga
     5941 cagcaccgat cgcgccgggc cagcatgtcc attcccagaa ttgcggcatg ggcgactttg
     6001 caaaagacta tgctttcggc gtcgacgtca cgccgacgcc gaatttcgat ctgcccgcga
     6061 ccttcgacgg catccgccgc cccgacgggc gggtggcgac ccgcaactat atcggcattc
     6121 tcacctcggt gaattgcagc gcgcatgtcg cgggcatggt cgcggatatc ttcaagaaga
     6181 acccattcac cggcgacaat ccgctggtcg actttcccaa tgtcgatggc gtggtcgcgc
     6241 tgacccacaa gaccggctgc ggcatgacgc aggacgagcc gctgaccatc ctgcgccgga
     6301 cgctcggcgg ctacgcgcgg catgtgaatt tttccgccgt ggtggtgctg ggcttgggct
     6361 gcgaggtcaa ccagatcggc gggttgatga aagagcagaa gctcgccggc cgcctgcgcg
     6421 aactggaaat tcaggaaatc ggcggcaccc gcaagaccgt cgaagccggc gtcgcctttg
     6481 tgcgcgaggc gctcaccgat gccaacaacg tcaagcgcga gccggtgtcg gccagcgagt
     6541 tgaccgtggc gctgcaatgc ggcggctccg acggttattc cggcgtctcc gccaatcccg
     6601 cgctgggtgc tgccagcgat ctgttggtgc ggcacggcgg caccgtcatt ttatcggaga
     6661 ccccggagac ctacggcgcc gaacatctgt tgacgcgccg cgcggtcagc cgcgaggtcg
     6721 gcgaaaaact ggtggcgctg atgcgctggt gggaagaata cacggcgcgg gaaggcgccg
     6781 agatgaacgc caacccgagc cccggcaaca aggccggcgg cctcaccacg atcctggaaa
     6841 agtcgctcgg cgcgatggcc aaggc
//
LOCUS       DASWGM010000851         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1897778,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000851 DASWGM010000000
VERSION     DASWGM010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897778"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VGA15_23230"
     CDS             complement(<1..874)
                     /locus_tag="VGA15_23230"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9590643.1"
                     /translation="MNARLERKLRSLAALVAAGLVAGIVFAIAQGRTSPSSYVVGASY
                     GLLMSVALGSTELFVLEGPMREWLGGLSFATSLMVRSTIYVAIITVIQWFQLGEVIAG
                     LSPDTSTFWSSFIYSAVLSVVMNLALGVTNIIGPRAFLNFFIGRYHTPVEEKRFVLFV
                     DIAGSTGLAERLGGIAIHRLLDRTFRLLTVAVVDYRGEVLNYVGDEVIVTWPERGGAI
                     DCRPLRCFVAMRDELSRASGQLEREFGAAPRIRGSLHFGPVIVGEIGDVKRAIVFNGD
                     VMNTAARLEELSRNV"
     gene            complement(959..1462)
                     /locus_tag="VGA15_23235"
     CDS             complement(959..1462)
                     /locus_tag="VGA15_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9590644.1"
                     /translation="MSTTSAMTLTRPEVRPFYAAIIAGLVCAFAIGKTLPIMMDTLSA
                     VVAPLCAVGDSSGSTPDTVEPITSHALPNVPGKRVTVVRVFYGPGGFTPAHRHAGSVT
                     AYITKGEIRSQLAGGPVETFKVGQSFFEPPGATHLVSANASATETAELIAVFVADEGA
                     QLTTFVE"
     gene            complement(1655..>2123)
                     /locus_tag="VGA15_23240"
     CDS             complement(1655..>2123)
                     /locus_tag="VGA15_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590645.1"
                     /translation="HMRRQGSGSIINNGSIAGRLAGFSSSMVYGAAKAAVIHLTKCVA
                     MELGESGIRVNSISPGAIATGIFGKALGLSVEAAEKTSDVMREVYKTAQPIPRAGLPE
                     DIAHAALFLASDESSFINGHDLVVDGAITGGRNWTQQQQGYVALRKAFDQGAG"
BASE COUNT          436 a          662 c          660 g          365 t
ORIGIN      
        1 ccacgttccg gctgagctct tcaagccttg ccgcggtatt catgacgtcg ccgttgaaaa
       61 cgatcgcgcg tttgacgtca ccgatctcgc cgacgatcac cggcccgaaa tgcaggctgc
      121 cgcgaatgcg cggggccgcg ccgaactcgc gctcgagttg gcccgatgcg cgcgacaatt
      181 catcgcgcat cgccacaaag catcgcagcg ggcgacaatc gatcgcgccg ccgcgttccg
      241 gccaggtcac gatcacctcg tcgccgacat aattgagcac ttcgccacga tagtccacga
      301 ccgcaacggt gagcagacga aacgtacggt cgagaagccg gtgaatggcg atcccgccaa
      361 gccgctcggc cagtccggtc gatccggcaa tgtcgacgaa caacacgaag cgtttctcct
      421 caaccggcgt atggtaccgc ccgatgaaga aattcaggaa tgcgcggggt ccgatgatgt
      481 tggttacccc gagagccaga ttcatgacga ccgagagcac cgcggagtag ataaagctgg
      541 accagaaagt tgacgtgtcg ggggacagtc cggcgatgac ctcacccaac tgaaaccact
      601 ggatgacagt gataatcgcg acatagatcg tgctccgcac catcagactg gttgcgaacg
      661 aaagaccacc gagccactcg cgcatcggtc cttccaggac gaacagttcg gtgctcccga
      721 gcgcgacgga catcagcagg ccatacgaag ctcccaccac ataagaggag ggcgaggtac
      781 ggccctgcgc gatggcaaac acgattcccg cgacgagccc ggcagcaacg agggcagcaa
      841 gagatcgcag ttttcgttcg agcctagcgt tcatgccgcc tacccccgcg ttgtcgtcgc
      901 agctgactat acacgggcgt ggccagcggc ccgaggcttg gagcgtggcg aggcatcgtt
      961 actcgacaaa ggtcgtgagc tgcgcgccct cgtccgccac gaacaccgca atcagttcgg
     1021 ctgtttcggt ggcgctggcg ttcgccgata ccaggtgcgt tgcgccgggc ggctcgaaga
     1081 aggattgccc gaccttgaag gtctcaacgg gaccgccggc aagctgcgag cggatttcac
     1141 ctttggtgat ataggcggta acagagcccg cgtggcgatg cgcaggggta aacccgccgg
     1201 gaccgtagaa tacccgcaca accgtcacgc gcttccccgg cacattgggc agcgcgtgcg
     1261 aggtgatcgg ttcgacggta tcgggcgtcg atccggagga atcgccgacc gcgcacagcg
     1321 gcgcaacaac cgccgaaagc gtatccatca tgatcggcag cgtttttccg atggcgaaag
     1381 cgcacaccag cccggcgata attgctgcat aaaaggggcg gacttcaggt cgcgtcagcg
     1441 tcatagcgga tgttgtggac atgatgatct cccgcagtct ggttcaaagc atatacaacc
     1501 cggattcgtc attccgggat ggtgcgtcag caccagaccc ggaatctcga gattccccga
     1561 tgtgcaattg cacatctgag gttcgatgcg gagcctgtca tcgggccggc cagaggccgg
     1621 acccgttggc atcgccccgg aatgacggcg gcggctatcc ggcgccctga tcgaacgcct
     1681 tgcgcaaggc gacatagccc tgctgttgct gggtccagtt gcggccgccg gtgatggcgc
     1741 catcgaccac cagatcatgg ccattgatga agctggattc gtcgctggcg aggaacagcg
     1801 cggcgtgggc gatgtcttcg ggcaaaccgg cgcgcggtat gggctgcgcg gtcttgtaga
     1861 cctcgcgcat cacatcggac gtcttctccg ccgcctcgac cgaaagtccg agcgccttgc
     1921 cgaagatacc ggtggcgatc gcgcccggtg aaatgctgtt gacgcggatg ccggattcgc
     1981 cgagttccat cgccacgcat ttggtgaggt gaatgaccgc ggccttggcc gccccataga
     2041 ccatcgacga cgagaaaccg gcgagccttc ccgcgatgct gccattgttg atgatgctgc
     2101 ccgacccctg gcggcgcata tga
//
LOCUS       DASWGM010000852        10013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2710644,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000852 DASWGM010000000
VERSION     DASWGM010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2710644"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGA15_23245"
     CDS             complement(<1..362)
                     /locus_tag="VGA15_23245"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9590646.1"
                     /translation="MTPFVQLVFDGPLARLTLKRGDKLNALDQAMIHALADAARTIDE
                     TTEARVTILNGEGKAFCAGGDIGAWGALPPLDMWRSWTRAGHRAFEALARLRTPLIVA
                     LTGHAFGGGLELAAAGDFR"
     gene            complement(359..1960)
                     /locus_tag="VGA15_23250"
     CDS             complement(359..1960)
                     /locus_tag="VGA15_23250"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX9590647.1"
                     /translation="MAKPRVLAASDAAELIPDGAIVTVSSSSALGCPDAVLAAIGTRF
                     DTHGHPRNLTTLHPIAAGDMWGVKGIDHIAKAGCLARVLAGSYPSGPSSAEPPTIWNM
                     ISDDAIPCYNVPSGILFDLHREAAAKRPGVLTKVGMDTFVDPIHEGCAMNAKARVAPI
                     VSRVNFAGEDWLFFPTIVPNVAIIRATTADERGNLSYEHEGGILGPLDQALSVRNNGG
                     LVIAQVKRLAAAGSLRPHDVVVPGVLVDVIVVAPDQKQTTNTPYDPAISGEIFRPLST
                     FETPAFGVGKTIARRVAQELRDGDAVNIGFGISANVPRILIEEGHHGAVTWMIEQGAV
                     GGIPLLDFQFGCASNAEAIVPSPAQFTYFQGGGFDMSLLSFLQIDRHGCVNVSRLGLR
                     PHVTAGAGGFVDITARAKKIVFSGFFTAGAALEIRDGALHVTREGKVKKLVEAVEQIS
                     FSGRRAVQQGQEIVYVTERCVMRLESAGLTVLEIAPGVNLERDVLAQSEFPLLVAPQL
                     KTMDANLFHPGPIRLQLRPAMEAVQ"
     gene            complement(1965..2975)
                     /locus_tag="VGA15_23255"
     CDS             complement(1965..2975)
                     /locus_tag="VGA15_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX9590648.1"
                     /translation="MSSGLRWGVIGASTIASEHMIGAIRANGGEVVAVLSSDASRGQS
                     YAETHGIPRSTTDLAALVEAPDIDAVYISTTNELHRDQVFAAAAAGKHVMCEKPLALT
                     LADARAMVAECKRQGVVMGTNHHLRNAATHRAMRDAIAEGRIGKPLFARVFHAVYLPP
                     RLQGWRIKTPGAGGGVILDITVHDADTLRFVLGDEPESVSAMVSRAGMGEAGLEDGVM
                     GVVRFAGGLLAQFHDAFTTRYATTGFEVHGDEGSLVATDCMTQQPKGTVVLRTSDGTV
                     PLSLIHENLYERSIRLFQDATAGAGVPAATGEDGLRSLALALATIGAAKAGRETSIAS
                     QG"
     gene            complement(2972..4036)
                     /locus_tag="VGA15_23260"
     CDS             complement(2972..4036)
                     /locus_tag="VGA15_23260"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX9590649.1"
                     /translation="MSETLRGDAAVRVGRVTIIDVARRAGVSKSTVSLVLSGSELVAE
                     ATKTRVNEAMQALGYVYHRGAASLRGASSDLVGMIMGDLNNPFFAELTAGIEDALFQR
                     GFTPILANTSEDTARQAQVLRAMREHNVAGIIMSPARGTDTWTLAQQWPSHIPAVITM
                     RRMIGSSLPYVGPDNQKGERAAVEHLLKLGHRRIAFIGGHENMTTQRERVAGWRAALN
                     GWGLAADESLIFEVPPTRDGGRIAIERAFDSPQPPTAFACYNDVVAMGASRALGLRGI
                     VVGRDIAVIGFDDIADAEHNAPPLTTVSADTREMGMRCAEGLLGLIRREDPASLSFAG
                     DARLIVRESCGAKFQGRGGA"
     gene            4217..5338
                     /locus_tag="VGA15_23265"
                     /pseudo
     CDS             4217..5338
                     /locus_tag="VGA15_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003587144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(5345..6436)
                     /locus_tag="VGA15_23270"
                     /pseudo
     CDS             complement(5345..6436)
                     /locus_tag="VGA15_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631264.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            6917..8047
                     /locus_tag="VGA15_23275"
     CDS             6917..8047
                     /locus_tag="VGA15_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX9590650.1"
                     /translation="MKSTLHLTGVACASLLLASVSAWAGPKIVAGPGANPGCFKPWSD
                     QTKFFQWDKKPGPYKIALVNGFVGNTWRIQMVKTAKAFAEQAGIKENIKEFKVVSTGT
                     DVAAQLGAMEDFINQGFDAIITIAVAPDGFDRIIRLADKNNVVVVPFDNILDTDKVMM
                     VNEDQKEMGRMSAKWLIDESGKKAGDILEVRGLPGNSVDRDRHIGFREIMEGAGNKFN
                     ITEVVGNWDTGTSQKVTADALAVHGHFDGVFTQGGSDGTVQAIMAAKHAFVPMSGEGE
                     NEFRKQIADHAKDGLKGMSYGQSPALVAIATKAAISALQGNVMPQLISIPIPVATYKD
                     LKPGTNYWPDLSANFFAPNQFLPCGVNFTAPEIMSQSEKNTQ"
     gene            8113..9657
                     /locus_tag="VGA15_23280"
     CDS             8113..9657
                     /locus_tag="VGA15_23280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX9590651.1"
                     /translation="MQDAVPTQRTFLTLSGISKRYGGVRALEGVDFACERGKIHAVLG
                     ENGAGKSTLIKIIAGVVQPDAGGMRLAGNDVSFATPSAANAAGVVCIFQELSLMPDLS
                     VADNISITSPPRRFGLIDARAQRRRAEALLAEIGCEDVNPLMRVRDLPLSRRQMVEIA
                     KALGQRPRLLILDEATSALTSADVEKVYAMLAKLKSEGIAILYISHRMHEVEALADRA
                     SVFRNGRHIETFDKGVRSTTDIVQLMIGRDIATQYPAKPARRPVKPVLALENLSWEKH
                     LHRISLGVGAGEIVGLGGLDGQGQKSLLLALFGVLRGVTGRITVEGREVRPGSPAAAK
                     SPAVGIALVPEDRKTEGLMLPMSIADNLAIASLDAISTGPFVDRAKERAAVERGIARL
                     QIKINARSDAVSTLSGGNQQKVVIAKWLMTEPRIILLNDPTRGIDVGTKQEMYQLLRE
                     LADAGAAILLYSTDYDELVGCCDRVAILYQGRILRELRGTEITEHNIVASALNLGLAG
                     GTEAAA"
     gene            9654..>10013
                     /locus_tag="VGA15_23285"
     CDS             9654..>10013
                     /locus_tag="VGA15_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003577215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590652.1"
                     /translation="MIELRLWVVQRSGMFLAIALFILMFGIYIGNHPAGLTVNVATTA
                     ANKGVLLALVAMAQTLPVLTGGLDLSVGMVFILCNCLASHIVIGSPLQVALGIIAVLA
                     TGLAAGAVSGTIVVYGRL"
BASE COUNT         1911 a         3201 c         3061 g         1840 t
ORIGIN      
        1 cgaaaatcgc cggcagccgc cagttccagg ccgccaccga aggcgtgacc cgtcagcgca
       61 acgatcaagg gcgtacgcag ccgcgcgagc gcttcaaacg cgcgatgacc ggcacgtgtc
      121 cagcttcgcc acatgtccaa cggcggcagt gcaccccagg cgccgatgtc gccgccagcg
      181 cagaacgcct tgccttcgcc attcaagatc gtgacgcgcg cctcagtcgt ctcgtcgatc
      241 gtacgagccg catcggcaag cgcgtggatc atggcctgat cgagcgcgtt gagcttgtcg
      301 ccgcgcttca gcgtcaatcg cgccagcggg ccatcgaata cgagttggac gaacggagtc
      361 attgtactgc ctccatggcc ggacgcagct gcagcctgat gggcccggga tggaacaagt
      421 tcgcatccat cgttttcagc tgcggagcga ccaggagcgg gaattccgat tgcgccagca
      481 cgtcacgctc gagattgacg cctggtgcga tctccaggac ggtcagcccc gctgattcta
      541 accgcatcac gcaacgctcg gtgacatata caatctcctg cccctgctga acagcacggc
      601 ggcccgagaa cgatatctgc tcgacggctt cgacaagttt cttgaccttg ccttcacgcg
      661 tgacgtgaag tgcgccgtcc cggatttcga gcgcagcacc tgccgtgaag aagcccgaaa
      721 agacgatctt cttcgcccgc gccgtgatgt cgacaaagcc acctgctccg gcggtcacat
      781 gaggtcgcaa tccaagcctg gagacattta cgcagccgtg gcgatcgatc tgcaagaagg
      841 acaacaacga catgtcgaag ccgccgccct ggaaataagt gaattgcgca ggtgatggca
      901 cgatcgcttc cgcattggag gcgcagccga actggaaatc caacagagga atgccaccga
      961 cagcgccctg ttcgatcatc caggtcacgg caccgtggtg accctcttca atcaggatac
     1021 gtggcacatt ggcggagatg ccgaagccga tattgacggc atcgccatcc cgcagctcct
     1081 gagcaacgcg gcgcgctatc gtcttgccga cgccaaacgc cggcgtctcg aacgtcgata
     1141 atgggcggaa gatttcgcct gaaatcgcgg gatcgtaggg cgtgttggtg gtctgcttct
     1201 gatcgggtgc gacgacgatc acatcgacga ggactcccgg gacaacgacg tcatgcggcc
     1261 gcaaggaacc agcggcggcg aggcgcttga cctgtgcgat caccaggccg ccgttgttgc
     1321 gcacgctcag cgcctgatcg agcgggccga gaatgccccc ctcatgctca taggagaggt
     1381 tgccgcgctc gtcggcagtg gtggcgcgga tgatggcgac gttggggacg attgtcggaa
     1441 agaacagcca gtcctcgccg gcaaagttaa cgcggctcac gatcggggcg acacgcgcct
     1501 ttgcgttcat cgcgcacccc tcatggatcg ggtcgacgaa cgtatccatg ccgactttgg
     1561 tcaatacgcc aggccgcttc gccgccgcct cgcgatggag gtcgaataga atgccagagg
     1621 gtacgttgta gcaagggatc gcgtcgtcgc tgatcatgtt ccagatcgta ggcggctccg
     1681 ctgatgacgg ccccgaagga taggagccgg caagaacgcg cgcgaggcag ccggcctttg
     1741 cgatgtgatc gatgcctttg acaccccaca tgtcgccggc ggcaatcgga tgcaacgttg
     1801 tcagattgcg cggatggccg tgggtgtcga agcgcgtgcc gatcgccgcc agaaccgcat
     1861 cggggcaacc caaagcagac gaggacgaga ccgtgacgat tgcgccgtct gggatgagct
     1921 cggctgcgtc gctggcggca agtactctcg gcttggccat tggatcaacc ctggcttgct
     1981 atggaggttt cccggcccgc ttttgcggcc ccgatcgtgg cgagagcgag cgccaacgag
     2041 cgcaagccat cctcgcccgt cgcggcgggt acgccggcgc cggcagtggc gtcctggaaa
     2101 agcctaatcg agcgctcgta aagattttca tggatcagtg acagcggcac ggtgccgtcg
     2161 ctggtacgca gcacgaccgt gcccttcggc tgctgcgtca tgcaatcggt cgcgacaagc
     2221 gatccctcat cgccatgaac ttcgaagccg gtcgtcgcat agcgcgttgt gaacgcgtca
     2281 tggaattggg cgagcaggcc gccggcaaag cggacgacac ccatcacgcc atcttcgagg
     2341 cctgcttccc ccatgcctgc gcgagacacc atcgcgctga cgctttcagg ctcatcgcca
     2401 agcacaaagc gcagcgtgtc ggcgtcatgg accgtgatat cgaggatgac gccgccgcct
     2461 gctccaggcg tcttgatgcg ccagccctgc aggcgcggcg gcaggtagac cgcatggaag
     2521 acccgggcga agagcggctt gccgatgcgt ccttcggcta ttgcgtcgcg cattgcccgg
     2581 tgggtcgcgg cattgcgcag atggtgattc gtgcccatga cgacaccctg gcgcttgcat
     2641 tctgcgacca tcgcgcgggc atcggccaag gtcagcgcga gcggcttttc gcacatcacg
     2701 tgctttccgg ccgccgcggc tgcaaacacc tgatcgcgat gcagctcgtt ggtggtcgag
     2761 atataaacgg catcaatatc aggcgcttcc accagcgcgg cgagatctgt cgtcgagcga
     2821 ggaattccat gggtttcggc gtagctttgc ccgcgtgacg catctgaact gagcaccgcg
     2881 acaacttcgc cgccgttcgc acgaatggcg ccgatcatgt gctctgaagc gatggtactc
     2941 gcgccgatca ccccccaacg caaaccgctg ctcatgcccc tcccctaccc tgaaacttgg
     3001 cgccacagct ctcgcgcacg atcaaacggg catcgccggc gaacgacagg ctcgcaggat
     3061 cctcgcggcg gatcaggccg agcaggccct cggcgcatcg catgcccatt tcccgcgtat
     3121 cggcactcac ggtcgtcagc ggcggcgcgt tgtgctcggc gtcggcaatg tcgtcgaacc
     3181 cgatcaccgc aatatcgcgg ccgaccacaa ttccgcgcaa accaagcgca cgcgatgcgc
     3241 ccatcgcgac gacgtcgttg taacaggcga acgcggttgg cggctgcgga ctgtcgaaag
     3301 cccgttcgat cgcgatacgc ccgccgtccc gcgtgggtgg gacttcaaag atcagggatt
     3361 cgtccgcggc gaggccccag ccgttgagcg cggcgcgcca gccggcgacg cgctcgcgtt
     3421 gcgtcgtcat gttttcgtga cccccgataa aggcgatacg acgatggccc agcttcagaa
     3481 ggtgctcgac agcggcacgc tcgcccttct gattgtcggg accgacatag gggagtgaac
     3541 tgccgatcat gcggcgcatc gtaatgacgg ctgggatatg ggatggccac tgctgggcca
     3601 gcgtccacgt atcggtgccg cgcgcagggc tcatgatgat cccggcgacg ttgtgctcgc
     3661 gcatcgcccg aagcacctgg gcctggcgcg cggtgtcttc gcttgtgttc gccaggatcg
     3721 gcgtaaagcc acgctgaaac agcgcgtcct cgatccccgc cgtgagctcg gcgaagaagg
     3781 ggttgttcaa atcgcccatg atcatcccga caagatcgga tgatgctcca cgcaggctgg
     3841 cggcgccacg atgatagacg tagccgagcg cctgcatggc ctcgttcacg cgtgttttcg
     3901 tcgcttcagc gaccaattcg ctgcccgaca agaccaacga aaccgttgat ttggaaacgc
     3961 cggcgcgacg cgcaacatcg atgatggtca cacgaccaac gcgaacagca gcatctcccc
     4021 tcagcgtctc ggacacttcc ctaacttttt ccctaacttt ctggatcgtt ccaattattt
     4081 ttactgacaa tttttccgca tgtttcctcg catatgcgaa gaaattctag tgcttcactc
     4141 ttgctaattg gaacgtgaca aaataagcct accgcagcat gcaaggatcg tcaacaaaga
     4201 tccagaggaa atgtcagtgg ctgattcgct ctgccagtcc gtgctcgatg cgctgacaga
     4261 tgttgatatg ccaaaacgtc atcccgcccg cacggctcgg ggatcgatcg aagctgaagg
     4321 cctgagattg ctggtttacg cctgcgacgc cctggtgctc ggcagcgggg cggcagggtg
     4381 gcgcgccgcc gtcgagttga agcggtacgg aatcgatgtc gttgtcgcga cgcaaaattg
     4441 cggcacatcg gcgtgctcgg gatcggacaa gcagaccctg cataccgccg cgaccggccg
     4501 gcatggcgat gatttcatcg cgcttgccgc agcccttggc gctggcggcg ccatggacga
     4561 agacacggcc tatgtggaag cggtgggatc gctgcgcgag ctgtcgtcgc tacaatatct
     4621 cggattgccg atcccgctcg accggctggg tgcggtgctc aggtatcaaa cggatcatga
     4681 cgaagccgag cgcgcgacga gttgcggacc ccgcacctct cgactgatgg tcaaggtgct
     4741 cgccgaggaa tcggcgcgtc tcggcgtgcc ggtcctgaat cgttgcgtcg gcgcgaagat
     4801 tttggcgacc gacgacaagc cgaagcgcgt cacgggcctg ctcgcattcc atgccgcgga
     4861 tcgcactgcc gacaacccgc tcgggcttgt cgtcatccga tgtgccgacc tcgtcaccgc
     4921 ggcaggcgga cccggcgaac tctatcgcga cagcgtctac cccaagcatt gtttcggcgc
     4981 cctgggactt gcgctcgaag ctggcgtgga ggcggtcaat ctgacggaaa gtcagttcgc
     5041 gatcggcacg ccgcgcgatg gatttcgctg gaatctgtcg ggaacgtacg cgcagtgcct
     5101 gccttacatc cattccgtcg ataccgatgg catcgagcgc aatttcctcg gcgactacta
     5161 tcgaacgacg caggagctcg cctccaacat tttccgcaag ggctatcaat ggccgttcca
     5221 tgcctcgcga atgctcgact tcggttctag ccttgtcgat gttgccatct ttcgcgagac
     5281 ccggatggga cggaaagtcc tgatggattt caatcggaat cctgagcctg ttccgggcgg
     5341 cgcgcctttg catcttgcgt gatggatctt ttgggcagca ctgaggtttg agcgggcata
     5401 catcgccgtc gagcttgctt gcacgatgca gaagtgtctt gccctcatgc actgtgccgc
     5461 tcgtcccaag ccgctttccg cccgggcagt tgtcggatca tattgaaagt cgctgcgcga
     5521 gaaggtgcca tcatctcgct tcgacttgtc gaacacgggg tatgtggcgc gatgcccttc
     5581 tcttcgacag gccagttcag catcggagct gccccgtaag cggtatcgcc gacaagccgc
     5641 tgcggtttga gaccgaagcg ctcctctgtt cgctcgatca tggtcttcgc cgcgctgacc
     5701 tcggcctgac gaatggcgcg cgaggcctcg acgtcaacga tgacgccgaa cttcacgtcg
     5761 atgagatagt tgtcggagta agcgaagaat gccggtccat tgtgcgcccc ggtccactgg
     5821 gctgccggat cggatagcga gacaaacttc gggacgacgt cgctggcggc gccccaggcc
     5881 gtatcatcga gggttgccag atgatccctc acagcactgg cgctcttcga atcgcggtcc
     5941 ttgtgccagt cctgacccgc gattgagcgt tgcttattgg cgtcagcctg gatcaggctc
     6001 gcatcgactg caaaaccttc gccaccaacc agaccggccg cgatacatgc ttcgacgacc
     6061 tgttcgaaca tacgacggaa aatatctccc tcgcggaagc gctcgttgcg ggcacgcgag
     6121 aacgccgaat gatccgggat agcatcctcg atgccgagct tgcagaacca gcgataggcg
     6181 aggttcacct gcacttcacg atagatcagc cgctccgagc ggatcgcaaa gacatacccc
     6241 atgaccagca tccggatcat cagttccgga tcgatgggcg acctatcgac gaataatgag
     6301 gggcaagttc gctgcgaagc caggacagat cgagagctgc atcgatccgc cgaacgaggt
     6361 gatcgtcagg caccgcatct gcgaggttaa actgagagaa aagttgaccc ggttcgctct
     6421 tcagacggcc catcatggat cgacctcgcc gagattcgcg tcagcacaac ggaatcacgg
     6481 ttcgagggct acctcaaccg acttctgcaa cgaaatccgc cagaagcgga cgtcctgctt
     6541 gttgagcagt tcgatcgggc cgtcggtact tcgctcttac ggttttaaat tgacgcgtaa
     6601 ctgcaatagt cgcgcgattg gcctgcttca atgcagttca aaaaggtttc ccactagctg
     6661 cccaaaaacc atatccgcga aggataatct ggtcagcgga accgttgtcg cccgagctaa
     6721 ctgctacagg gatgttcgcc aatcgctctt ggtaggtcgg aaacttgggc ccctgccgct
     6781 taccgtctgc attcggcttg tgcgtatcgc accttgcgtg gcatgctagt cgggtaagtg
     6841 caacgggaag cccaattccc ggcatggcga tcgaactgct cagggtcgga aaacagaaca
     6901 ctcagggaga agagacatga agtcgacact tcatcttacg ggggttgctt gcgcgagcct
     6961 cctgctggcc tcagtcagtg cttgggcagg ccccaagatt gtcgccggac cgggtgccaa
     7021 tcccggctgc ttcaagccct ggtcggatca gaccaagttc ttccagtggg acaagaagcc
     7081 cggcccctac aagatcgccc tcgtcaacgg cttcgtcggc aatacctggc gcatccagat
     7141 ggtgaagacc gccaaggcct ttgccgagca ggcgggcatc aaggagaaca tcaaggagtt
     7201 caaggtggtc tcgaccggca ccgatgtcgc ggcgcaactc ggcgccatgg aggattttat
     7261 caatcaaggc ttcgacgcca tcatcaccat cgcggtggcg cccgacggct tcgaccgtat
     7321 catccgcctc gccgacaaga acaatgtggt ggtggtgccc ttcgacaaca tcctcgacac
     7381 cgacaaggtg atgatggtca acgaggacca gaaggagatg ggccgcatgt cagccaaatg
     7441 gctgatcgat gaatcgggca agaaggccgg cgatatcctc gaggtgcgcg gcctgccggg
     7501 caattcggtc gaccgtgacc gccatatcgg cttccgcgaa atcatggaag gcgccggcaa
     7561 caagttcaac atcaccgagg tggtgggcaa ttgggacacc ggcacctcgc agaaggtgac
     7621 cgccgatgcg ctcgccgtgc acggccattt cgacggcgtg ttcacgcagg gcggctcgga
     7681 cggcaccgtg caggcgatca tggccgccaa gcatgctttc gtgccgatgt cgggcgaggg
     7741 cgagaacgag ttccgcaagc agatcgccga tcacgccaag gacggcctga agggcatgtc
     7801 ctacggccag tcgccggcgc tagtcgccat cgccaccaag gcggcgatct cggcgctgca
     7861 gggcaatgtc atgccgcagc tgatctcgat cccgatcccg gtcgcgacct acaaggatct
     7921 caagcccggc acgaattact ggcctgacct cagcgccaac ttcttcgctc cgaaccagtt
     7981 cctcccgtgc ggcgtgaatt tcacagcgcc cgagatcatg tcgcagagcg agaagaacac
     8041 ccagtgaccg ctgcggctac gttccagtca cggcctggca tccgccaggc cgtcgctgcc
     8101 tgagcccgca ccatgcagga tgcggtcccg acgcaacgaa ccttcctcac attgtccggc
     8161 atctccaagc gctacggtgg cgtgcgcgcc ctcgagggcg tcgacttcgc ctgcgagcgc
     8221 ggcaaaatcc atgcggtgct gggcgagaac ggtgccggca agtcgacgct gatcaaaatc
     8281 atcgccggcg tggtgcagcc cgatgccggc ggcatgcgcc ttgccggaaa cgatgtcagc
     8341 ttcgccacgc cgtccgccgc caacgcggct ggcgtcgtgt gcatcttcca ggagctgtcg
     8401 ctgatgcccg acctctcggt cgcggataat atctcgatca cgtcgccgcc gcggcgcttc
     8461 ggcctcatcg acgccagggc gcagcgccgc cgcgccgagg ccctgctggc cgagatcggc
     8521 tgcgaggacg tcaacccgct gatgcgggtg cgcgacctgc cgctgtcgcg gcgccagatg
     8581 gttgagatcg ccaaggctct cggccaaagg ccgcggctcc ttatcctcga tgaggccacc
     8641 tcggctctca ccagcgccga cgtggagaag gtctatgcga tgctcgcgaa actcaaatcc
     8701 gagggcatcg ccatcctcta catctcacac cgcatgcacg aggtcgaggc gctcgccgac
     8761 cgggcctcgg tgttccgcaa cggacgccat atcgaaacct tcgacaaggg cgtgcgctcg
     8821 accacggata tcgttcagct gatgatcggg cgcgacatcg ccacccaata tccggcaaag
     8881 ccggcgcgtc ggccggtgaa gccggtgctg gcactcgaga acttgtcctg ggagaagcat
     8941 ctccaccgca tttcgctcgg cgtcggcgcc ggcgagatcg tcggcctcgg cggcctcgat
     9001 gggcagggcc agaaatcgct gctgcttgcc ttgttcggcg tgctgcgcgg cgtcaccggg
     9061 cgcatcacgg tcgagggccg cgaggtgcgc cccggctctc cggcggcggc gaagtcgccc
     9121 gcggtcggca tcgcgctggt gcccgaggat cgcaagaccg aaggattgat gctgccgatg
     9181 tcgatcgccg acaatctggc gatcgcatcg ctcgatgcca tctcgaccgg gcctttcgtc
     9241 gatcgcgcca aggagcgcgc ggcggtcgag cgcggcatcg cccggctgca gatcaagatc
     9301 aacgcacgaa gcgatgcggt ctcgaccctg tcgggcggca accagcagaa ggtggtgatc
     9361 gccaaatggc tgatgaccga gccccgcatc atcctgctca acgatccgac gcgcggcatc
     9421 gatgtcggca ccaagcagga gatgtatcag ctgctgcgcg agctcgccga tgccggcgcc
     9481 gccatcctgc tttacagcac cgactacgac gagctggtcg gctgctgcga ccgcgtcgcc
     9541 atcctctatc agggacggat cctgcgcgag ctgcgcggca ccgagatcac cgagcacaac
     9601 atcgtcgcca gcgcgctcaa tctcggcctc gccggcggca ctgaggccgc ggcatgatcg
     9661 agctgcggct atgggtcgtc cagcgctccg gcatgttcct cgcaatcgcg ctgttcatcc
     9721 tgatgttcgg tatctatatc ggcaaccatc cggccgggct gaccgtcaac gtcgcgacga
     9781 ctgccgccaa taagggcgtg ctcctcgccc tcgtcgccat ggcgcagact ctgccggtgc
     9841 tgaccggcgg cctcgacctc tcggtcggca tggtgttcat cctgtgcaac tgcctcgcct
     9901 cgcatatcgt catcggcagc ccgctccagg tcgcgctcgg catcatcgcg gtgctcgcca
     9961 ccggcctcgc cgccggcgcc gtcagcggca cgatcgtcgt ctatggccgg ctc
//
LOCUS       DASWGM010000853         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2710864,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000853 DASWGM010000000
VERSION     DASWGM010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2710864"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VGA15_23290"
     CDS             complement(<1..1142)
                     /locus_tag="VGA15_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX9590653.1"
                     /translation="MTRFAPPPTLRRTALGTSALVTLALAVGLSALAAADAWAKQKVP
                     MPKPRPIARNLVPKTPAPTTPPPTTSAPTSPVPKTAAKNTTPAPNPAAKNTATAAPAA
                     PAMPPATRQHAALPPAPARRPVAPAAVAATTSTSQSDTEALESVIDLIRKHKPADATQ
                     AEAVISDPVAKKLAEWIILRSDDNGASVERYRAFIAANPSWPSQSFLRRRLEAALWDD
                     HRDDATVWAWFENESPLSAKGRFSLARVMIARGDRANAERLVREAWRNDTMSEDTEST
                     ALDLFGALLTPGDHKARMDTMLYGSEQEAGLRAAKRLGAAHVALAKARNAAYRKAPNT
                     KALLDAVPHELHGDPGYIFSRIQLLRRDEKFAEAAQLMLSAPKDPGR"
     gene            1501..2391
                     /gene="dapA"
                     /locus_tag="VGA15_23295"
     CDS             1501..2391
                     /gene="dapA"
                     /locus_tag="VGA15_23295"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX9590654.1"
                     /translation="MAAKTNFRGSFTALVTPFKNGSLDEAAFRALVNWQIAEGTHGLV
                     PVGTTGESPTVNHDEHKRVVEWCIDEAGGRVPVIAGAGSNSTKEAIELAEHAERSGAS
                     AVLVVTPYYNKPTQEGMYQHFKAINDAIGIPIIIYNIPPRSVVDMSVETMARLFELKN
                     IAGVKDATASMVRVSQQRASLGEDFNQLSGEDATVLGYMAHGGHGCISVTSNVAPRLC
                     SEFHLAWQKGDLATALKLHDKLMPLHTNLFIESNPAPVKYALSLLGKIDEKLRLPMVP
                     VTEPTRLAVRSAMVHAGLIN"
     gene            2444..2857
                     /gene="mscL"
                     /locus_tag="VGA15_23300"
     CDS             2444..2857
                     /gene="mscL"
                     /locus_tag="VGA15_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX9590655.1"
                     /translation="MLKEFREFAMKGNVVDLAVGVIIGAAFGVIVTSLVGDIIMPIIG
                     AITGGLDFSNYFTGLSKAVTAGNLAEAKKQGAVLAWGNFLTLTLNFIIIAFVLFVVIR
                     SMNKLKRKDEAAPAPPKPTRQEELLTEIRDLLKKS"
     gene            2924..>2988
                     /locus_tag="VGA15_23305"
     CDS             2924..>2988
                     /locus_tag="VGA15_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HEX9590656.1"
                     /translation="MAEKNERAIKVVAENRKARFN"
BASE COUNT          546 a          898 c          980 g          564 t
ORIGIN      
        1 cggcccgggt ccttcggtgc gctcagcatc agctgcgcgg cttcggcgaa tttctcatcg
       61 cggcggagca gctgaattct gctgaagatg tagccgggat cgccatgcag ttcgtgcgga
      121 accgcgtcga gcagcgcttt ggtgttgggc gccttgcgat aggccgcgtt ccgggctttc
      181 gccagcgcaa cgtgtgccgc gcccaatcgc ttggcggcgc gcaggccggc ttcctgctcg
      241 ctgccgtaga gcatcgtatc catccgcgcc ttgtggtcgc ccggcgtcag caatgcgccg
      301 aacagatcga gcgccgtgct ctcggtatct tcggacatgg tatcgtttcg ccaggcctcg
      361 cgcaccaggc gctccgcatt ggcgcggtcg ccgcgcgcga tcatgacccg cgccagcgag
      421 aatcggcctt tggccgacag cggcgattcg ttttcgaacc aggcccacac ggtggcgtcg
      481 tcgcgatggt cgtcccaaag tgctgcttcg aggcgccggc gcaggaagct ctgcgagggc
      541 caactcggat tggccgcgat gaatgcgcgg taacgctcga ccgaagcgcc gttgtcgtcg
      601 ctgcgcaaga tgatccattc cgcgagcttt ttggcgaccg ggtccgaaat caccgcttcg
      661 gcttgggtcg catccgcggg cttgtgcttg cggatgagat cgatgacgct ttcgagcgcc
      721 tcggtatcgg actgcgaagt cgatgtggtc gcggcaaccg ccgccggcgc taccggtctg
      781 cgcgccggtg caggcggcaa ggccgcatgc tggcgtgtcg cgggtggcat ggcaggcgct
      841 gccggagctg cggtcgcggt atttttggcg gccgggtttg gcgcaggcgt ggtatttttg
      901 gccgcggtct tggggacggg actcgtggga gcggaagtcg tgggaggagg ggtcgtggga
      961 gcaggagtct tgggcacgag gttgcgggcg atcggccgcg gcttgggcat gggaactttt
     1021 tgcttggccc aggcgtccgc cgccgccaat gccgacaatc ccaccgccag cgccagcgtc
     1081 accagcgcgc tggtgcccaa agcggtccgt cgcaatgtgg gtgggggggc gaatcgcgtc
     1141 acggcgttcc ttcgcggcga atcaatcaag cgctgaagcc ctagctctat ttaattgaat
     1201 atatggacaa aatactaaat acgcggggat tggcgatgtc tgcgccatta ccgcggcaac
     1261 atcggggcta cagcgttttc aagcgaaagc ctgcccccgg acgcgatccg gggtggagac
     1321 cggttcgcgt caagaaaacg cgtcaaatca agaatctaga gccccgtttc gattcgatcg
     1381 aaacggaaaa ggctcgaggg tcggaccggc gcgggccttt cgcccgatcc tcagacccgt
     1441 tatgaattgg tccttatcgc cggttcagac ggcaggtggc gtgcgttcgg gagaaagtcc
     1501 atggcagcca agacgaattt ccggggctcg ttcaccgcct tggtcacgcc attcaagaac
     1561 ggctcgctcg acgaagcggc ctttcgcgcc ttggtgaact ggcaaatcgc cgagggcacc
     1621 cacggcctgg tgccggtcgg cactaccggc gaaagcccca ccgtgaacca cgacgagcac
     1681 aagcgcgtcg tcgaatggtg catcgacgag gccgggggcc gggtgccggt gatcgcgggc
     1741 gccggttcca attcgaccaa ggaagccatc gaactcgccg agcacgccga gcgctccggc
     1801 gccagcgcgg tactggtggt gacgccgtac tacaacaagc cgacccagga aggcatgtac
     1861 cagcacttca aggcgatcaa cgacgcgatc ggaattccga tcatcatcta taacattccg
     1921 ccgcgctcgg tggtcgatat gtcggttgag accatggcgc ggctgttcga actaaagaac
     1981 atcgccggcg tcaaggatgc gaccgcgagc atggtgcggg tgtcgcagca acgcgcaagc
     2041 ctcggcgagg atttcaacca gttgtcgggc gaggacgcca ccgtgctcgg ctacatggct
     2101 catggcggtc acggctgcat ttccgtcacg tccaacgtcg cgccgcggct gtgctccgaa
     2161 tttcacctgg cctggcagaa gggcgatctc gccaccgctc tcaagctgca cgacaagctg
     2221 atgccgctgc acaccaatct cttcatcgag tccaatccgg cgccggtgaa atacgccctg
     2281 tcgctgctcg gcaagatcga cgagaagctg cgcctgccga tggtgccggt caccgagccg
     2341 acgcggttgg cggtgcgcag cgccatggtg catgcgggcc tcatcaacta agggcttcga
     2401 cggtccggcc gtggtgagcg acgtcgaaga gaaggggctg cagatgctca aggaatttcg
     2461 cgaattcgcg atgaagggca acgtggtcga cctcgcggtc ggggtcatca tcggcgcggc
     2521 gtttggcgtc atcgtcactt cgctggtcgg tgacatcatc atgccgatca tcggcgcgat
     2581 taccggtgga ttggatttct ccaactattt caccggcctg tcgaaggcgg tcaccgccgg
     2641 caatctggcc gaagcgaaaa agcagggcgc ggtattggct tggggcaact ttctgacgct
     2701 cactctgaac ttcatcatca tcgcctttgt gctgttcgtg gtgattcgct ccatgaacaa
     2761 gctgaagcgg aaggatgaag ccgcgccagc gccgccgaaa ccgacccgtc aggaagagct
     2821 gctgaccgaa atccgcgatt tgctcaaaaa gtcttgacgt gcgcacgggc gggttgagta
     2881 ttaccgctct ccgtcgaaga tagatcccaa ggcattcttc atcatggccg aaaagaacga
     2941 gcgcgcgatc aaggtcgtcg ccgaaaatcg caaggcccgg ttcaacta
//
LOCUS       DASWGM010000854         6311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1192964,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000854 DASWGM010000000
VERSION     DASWGM010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192964"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VGA15_23310"
     CDS             <1..659
                     /locus_tag="VGA15_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9590657.1"
                     /translation="VATAAEELSSSISEISRQVAQSSNIAARAVDSARRTDSIVRALA
                     DGAHQIEHVAELISSIAGQTNLLALNATIEAARAGDAGRGFAVVAAEVKSLTSQTAEA
                     TKEIGTRITQIQSATKEAVEAIQGITATIEEVSAIATTIGSAIEEQGAATAEIARNVT
                     QTAQATQEVTTNIGGVSAAANETGGAAGLVLSAASNLSKHAEQLSGEVNVFLAGVRAA
                     "
     gene            complement(710..4408)
                     /locus_tag="VGA15_23315"
     CDS             complement(710..4408)
                     /locus_tag="VGA15_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX9590658.1"
                     /translation="MPAPKRSLPVGGRGLGGDQSYNQRPHREAMARNTSPKGSNPRAD
                     VRLSQWDDAGWDQDDAAGRRARRLLSNPSSGLHRAGDYAGGLRRWLATERWVKRIAII
                     IAVLMVIFAGSFGGLWWRLGAGPINLDMATPWLAAAIEENIGHGNTVEVGGTQIERAG
                     RIRIAVRIRDIIVRDRDHVIVASAPKAEVRLSGTALLMGQLRAESLNLVDAELAVRIT
                     PDGYVTVSAGDTAKPLATGVTSKKQAGIPATFPRQIPAAPPGAAAPSSPAPAAGPDTP
                     SGLLAGLDWLDSLSLTGLDGQNLNEIGLKNGNLIVDDQQRGNKWNFENITLSLRRPSG
                     GGVALSVGEEGSKAWSLRVVVGPPANGVRSVDIRANKVSTANILLALRTKALTYSADL
                     PLTGELKGELGRDGLPTYFSGKLTAGEGHIIDTDTPDYPMAIDSAEISVEWDSGRRVL
                     VAPFKIISGANRVTLLAHLEPPNDSVTDWQLGFSGGTIVLAGDNGEQPLIFNRIAIGL
                     RFDTDKKRVLLTQADISNGEIGIAGTGSVDYSAEARLQLGFAGTPMSAAALKRMWPIL
                     IVPEVREWVIERIERGSLQRIEVGVNSPVRNLSRRGPPIPDDGLAVNIVASGVTLRPV
                     DELPSVRDADLKAHVTGRTATVTIGQGAVDTPAGRKLNITDFVFEVPDMAPKPSPARV
                     KFRIDGPVPAVAEILASDRLSEFSGTLIDPNSSKGTVAALVTLGLPIKRELSKADTSY
                     TITADLGGFGADRLVMNQKLEADKLKVVANNQGYQVKGDVKINGQPASLDYRKPNDGD
                     ADIKLQATLDDASRARLGMDLGPAVTGNLPIKLIGKIGGPDRDSRMGIEADLTALRLD
                     NILPGWVKLPGKSSRAVFNVVQKPQSTRFEDIVIDGGGVSIKGSLEVDQNGDLINVNF
                     PTYSPSEGDKTSLKAERGSDGVLKVTMRGDVFDGRGFLKSAMSGKEADAKSKTRNVDF
                     DVDVKLGAVAGHNGEAVRSLDVKLSRRNGTIRSFALNGKLGRDTPLTGNFGRPAQGRE
                     VIYLETNDAGAFFRFTDTYSKVVGGQLQLTMDPPTAEPKAREGLINIRNFSVKGEASL
                     DRIAAGGPTGVQNGVSFSALRAEFTWQNGQLTIRDGVVKGPMVGGTIGGNIDFPANQV
                     RMNGTFIPMYGLNNMFGQIPIVGLFMGGSNEGLFGVTYEVVGTTDKPTLRVNPISAIF
                     PGVSRKIWEFNPGKQNNPVEFPAAPSNN"
     gene            4618..5409
                     /locus_tag="VGA15_23320"
     CDS             4618..5409
                     /locus_tag="VGA15_23320"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9590659.1"
                     /translation="MSKKTRKKSSKPSSGKTTRVQAKVSGKSPLKTRNATVKKSGKSA
                     DKPSGSAAARASHKAPSKKSKSPRAGESMSRAAAESKTAAARPRAKSPAASKPSGGKP
                     DASAGLKSELAEGAKAPAFRLPRDGGETISLADYSGKKLVLFFYPRADTPGCTREAID
                     FTRLADAFAENQTAIVGVSADPLKAQEAFRDKHQLSVPLVSDEAHEMLESYGVWGEKS
                     MYGRTFQGILRTTVLIGADGRIAKIWRNVKVDGHADDVLAAARTL"
     gene            5511..>6311
                     /locus_tag="VGA15_23325"
     CDS             5511..>6311
                     /locus_tag="VGA15_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEX9590660.1"
                     /translation="MSNRSGHYSEHTLHHHQHDHARAALRRPAASQASVSSPSQAAEA
                     GYTLVHAGKQVRFGPVVFWIVVGTVTMLGMWSAATATYFAFRDDVLTRLIARQAEMQY
                     AYEDRIAELRAKVDRTTSRQLLDQQQFDQKLDQIMRRQTALESRATALGAIPDASVTG
                     SIRAPSRGAAATDAAASSGIPKPSPINDTVIFVAPPDREARLESRAPVIVNPRSSQFT
                     KVQGVDSVLARLQGSLDQIEGRQIAALSSVEESMESRVRRMRGVFTDLG"
BASE COUNT         1192 a         2047 c         1911 g         1161 t
ORIGIN      
        1 ccgtcgccac ggcggcggaa gaactgtcct cgtcgatcag cgaaatcagc cgtcaggtcg
       61 cacaatccag caatatcgcc gcgcgcgcgg tcgacagcgc acgccgtacc gacagcatcg
      121 tgcgcgcttt ggccgatggc gcccaccaga tcgagcatgt ggcggaactg atttcaagta
      181 tcgcgggaca gaccaatctg ctcgcgctca acgccaccat cgaagccgcc cgcgcgggcg
      241 atgccggcag aggcttcgcg gtggtggcgg ccgaggtcaa gagcctcacc agccagaccg
      301 cggaagccac caaggagatc ggcacccgca ttacccagat ccagagcgcc accaaggaag
      361 ccgtcgaggc gatccagggc atcaccgcca cgatcgagga agtcagcgcg atcgcaacga
      421 cgatcggctc cgcgatcgag gagcaaggtg ccgctaccgc ggagatcgcc cgcaacgtga
      481 cccagacggc gcaggcgacg caggaggtaa cgacgaatat cggtggcgtc agcgccgcgg
      541 ccaacgaaac cggtggcgcc gccggcctcg ttctttccgc cgcctccaat ctctccaagc
      601 acgccgagca actgtccggc gaggtcaacg tcttcctggc tggcgtacgc gcggcctgag
      661 ggattcgttc ttcgtcgtag gtgctgcttc cggtcgcagc tcgtcgaggt cagttgttgc
      721 tcggcgccgc cggaaattcg accgggttgt tttgcttgcc ggggttgaac tcccagattt
      781 tcctcgagac tccggggaat atggccgaaa tcggattgac gcgaagcgtt ggcttgtcgg
      841 tggtcccgac gacctcatag gtgacaccga acagcccctc attgctgccg cccatgaaca
      901 gtccgacaat cggaatctgg ccgaacatgt tgttcagccc gtacatcgga atgaaggtgc
      961 cgttcatacg cacctggttg gcggggaaat cgatgttgcc cccgatggtc ccgccgacca
     1021 tcggaccttt cacgacgccg tcccggatcg tgagttggcc gttctgccag gtaaattccg
     1081 cgcgcaatgc cgagaaggaa acgccattct gcactccggt tggcccgccc gcggcgatgc
     1141 gatcaagcga agcctcgccc ttgaccgaga aattgcggat gttgatcagc ccctccctcg
     1201 ccttcggttc ggctgtcggc ggatccatcg tcaattgaag ctggcctcca accaccttgg
     1261 agtaagtatc ggtgaagcgg aagaaggcgc cggcgtcgtt ggtctcgaga tagatcacct
     1321 cgcgtccctg cgcgggcctc ccgaaatttc cggtcagtgg tgtgtcgcgg ccaagcttgc
     1381 cgttgagcgc gaagcttctg atcgttccat tgcgccgcga taatttgaca tcgaggctgc
     1441 gcaccgcttc gccattgtgg ccggctaccg cgccgagctt cacgtcgaca tcgaaatcga
     1501 cgttcctggt cttgctcttg gcgtcggcct ccttgcccga catggcggat ttgagaaaac
     1561 cgcgaccgtc gaacacatcg ccgcgcatcg tcactttcag cacgccatcg gagccacgtt
     1621 cggccttcag cgaggtcttg tcaccctcgg atggagaata ggtcgggaag ttgacgttga
     1681 tcaggtcgcc gttctgatcg acctcgagcg atcccttgat cgacacgccg ccgccatcga
     1741 tgacaatgtc ttcgaagcgg gtcgattgcg gtttctgcac cacgttgaaa accgcgcggc
     1801 tcgacttgcc cggcaacttc acccagcccg gcaggatatt gtcgagcctc aacgccgtca
     1861 gatcggcttc gatccccatg cgactgtcgc gatcgggacc gccgatcttg ccgatcagct
     1921 tgatcggaag attcccggtg acggcggggc cgagatccat accgagccgc gcgcggctgg
     1981 cgtcatcgag cgtcgcctgc aatttgatat ccgcgtcgcc gtcgttgggc ttgcgataat
     2041 ccagtgaggc cggttgccca ttgatcttga cgtcgccctt gacctgatag ccctggttgt
     2101 tggcgacgac tttcagcttg tcggcttcga gcttttgatt catcaccaag cggtcggcgc
     2161 cgaatccgcc aaggtcggcc gtgatggtgt agctggtgtc ggccttggac agttcgcgct
     2221 tgatcggcag gccgagcgtg accagggcgg caacggttcc cttgctcgaa ttaggatcga
     2281 tcaaagtgcc ggaaaattcg ctgagacggt cggaggcgag gatttccgcc accgccggca
     2341 ccgggccatc gatccggaat ttaacgcgag ccggcgaggg ttttggcgcc atgtccggca
     2401 cctcgaacac aaaatcggta atgttcagtt tgcggcccgc gggggtgtcg accgccccct
     2461 gtccgatcgt cacggtggcg gtgcggccgg tgacatgcgc tttcaaatcc gcatcgcgca
     2521 ccgacggcag ctcgtccacc ggtcgcaatg tcacgccgct ggcgacgatg ttgacggcga
     2581 gaccgtcatc gggaatcggc ggacctcgcc gcgagagatt gcgaaccggc gagttgacgc
     2641 caacctcgat gcgctgcagc gagccgcgct cgatgcgctc gattacccat tcgcgcacct
     2701 ccggcacgat caggatcggc cacatccgct tcagcgcggc cgccgacatc ggcgttcccg
     2761 cgaaaccaag ttggaggcgc gcctccgccg aataatcgac gctgccggtt ccggcgatac
     2821 cgatctcgcc gttgctgata tcggcttgcg tcagcagaac gcgcttcttg tcggtgtcga
     2881 agcgcaggcc gatggcgatg cggttgaaga tcaagggctg ttcgccgttg tcccccgcaa
     2941 gcacgatggt gccgccactg aaacccagct gccagtcggt aacactgtca ttgggcggct
     3001 cgagatgagc cagcagcgtg acccggttcg caccggaaat gatcttgaac ggcgcgacca
     3061 gcacgcgccg tccggaatcc cattcgacac tgatttccgc tgaatcgatc gccatcggat
     3121 aatccggcgt atcggtgtcg atgatgtggc cttcgcccgc ggtgagtttg ccactgaaat
     3181 aggtcggcag cccgtcacgg cccagctcgc ccttgagttc gccggtcagc ggcaaatccg
     3241 cgctgtaggt gagggccttc gtccgcagcg ccagcaggat attcgccgtc gaaaccttgt
     3301 tggcacgaat atcgaccgat ctcacgccgt tcgcgggcgg acccaccacg acgcgcagcg
     3361 accaggcttt gctaccctcc tcgccgacgc tcagcgcgac tccgccgccg ctcggccgcc
     3421 gaaggctgag ggtgatgttt tcgaaattcc atttgttgcc gcgctgctga tcgtcgacga
     3481 tcaggtttcc gttcttcaac ccgatttcgt tgaggttctg tccgtcaagt ccggtcaggc
     3541 tcaggctgtc gagccaatcg aggccggcga gcagcccgct tggcgtatcg ggacccgcgg
     3601 caggcgccgg cgaggacgga gcagccgctc cgggtggtgc agcggggatt tggcgcggaa
     3661 aggtggccgg aatacctgcc tgtttctttg aggtcacacc ggtagccaaa ggtttggcgg
     3721 tgtcgccggc ggaaaccgtc acatagccgt ccggggtgat gcgcaccgcg agttcggcgt
     3781 ccaccagatt gagactttca gcgcgaagtt gtcccatcag cagcgcggtg cctgacagcc
     3841 tcacctcggc cttcggcgcg ctcgccacga tgacgtgatc gcgatcgcgg acgatgatat
     3901 cgcggatacg cacggcgatg cggatccggc cggcgcgctc gatctgcgtc ccgccgacct
     3961 cgacggtgtt gccgtggccg atattttcct cgatcgccgc ggccagccac ggcgtagcca
     4021 tatcaagatt gatcggaccc gcgccgagcc tccaccacag cccgccgaag gaccctgcga
     4081 agatcaccat caagaccgcg atgatgatgg cgatccgttt gacccagcgt tcggtggcaa
     4141 gccaccggcg tagccctccg gcatagtcgc cggcccggtg aagcccggaa ctcggattgg
     4201 acaacagccg gcgcgcgcga cggcccgcgg catcgtcttg atcccaaccg gcatcatccc
     4261 actgcgagag ccggacgtca gcacgtggat tcgatccctt gggcgacgtg ttccttgcca
     4321 ttgcctctcg gtgcgggcgc tggttgtaag attgatcgcc gccaaggccg cgcccgccga
     4381 caggtaacga gcgcttcggt gccggcatcg ctgctgccaa tccctcgatt aatactgtaa
     4441 cttttcgtcg ggcccaagga acgcattcag cgagccgacg ggtcgtgcgt cgaattcctt
     4501 gtaaccatac tcccagacca tcaaacttgc ccagcagtcg gtcttattcc ggcacgctgg
     4561 ggcaaggccc gcaatactcc catgattgat ccgccgaaag cgacgaaagg aaggcgtatg
     4621 tccaagaaaa cgcgcaagaa atcctccaaa ccctcctccg gcaagacgac ccgcgtccaa
     4681 gcgaaggttt cgggcaaatc gccgctgaag acccgcaacg caacagtgaa aaagtcaggc
     4741 aagagcgcag acaagccatc cgggagcgcc gccgcgcgag catcacacaa ggccccatca
     4801 aaaaagtcaa aatcgccccg tgccggcgaa tcgatgtcgc gggccgcggc cgaatcgaag
     4861 acagccgctg cgaggcccag ggcaaaaagt ccggctgcaa gcaaaccgtc aggcgggaaa
     4921 cccgacgcgt ccgccggcct taaatctgaa ttggccgagg gcgcaaaagc gccggccttc
     4981 cgcctgccgc gcgatggcgg cgaaaccatc tcgctggccg actattcggg taaaaaactc
     5041 gtgctgtttt tctatccacg cgctgacact ccgggctgca ccagggaggc catcgacttc
     5101 acccggctgg ccgacgcgtt cgcggaaaat cagaccgcga tagtgggtgt ttcggccgac
     5161 cccctgaagg cgcaggaagc cttccgggac aagcaccaac tctccgtgcc tctggtttca
     5221 gatgaagcgc acgagatgct ggaaagctac ggcgtctggg gggaaaaatc catgtatggc
     5281 aggaccttcc agggcatcct tcgcaccacg gttctgatcg gcgccgacgg ccgaatcgcc
     5341 aaaatatggc gcaatgtaaa ggtcgacggc cacgccgatg acgtgcttgc ggccgcccgt
     5401 accctttagt ttgagcgatc ccattcgctg aaaattaacc atgacagggt caaatcacac
     5461 ccgcaaattt ggccgtacgg aactcctatc cgaccggcgc gggagtgccg atgtcgaacc
     5521 gttccggtca ttattccgaa cataccctgc accatcatca acatgaccac gccagggcag
     5581 ccttgcgccg cccggcggcg agtcaggcgt cggtctcttc cccttcacaa gccgcggagg
     5641 ccggctatac cctcgtccac gccggcaagc aggtccggtt tggtccggtg gtgttctgga
     5701 tcgtggtcgg cacggtgacc atgctcggca tgtggtcggc cgcgaccgcg acctatttcg
     5761 cgtttcgcga cgatgttctc acccggctga tcgcacgcca ggcggagatg caatacgcct
     5821 acgaagaccg catcgccgaa ctgcgtgcca aggttgatcg caccaccagc cgtcaattgc
     5881 tcgatcagca gcaattcgac cagaaactcg atcagatcat gcggcgccag accgcgctgg
     5941 aatcgcgcgc cacagcactt ggcgccattc cggacgcctc ggttaccgga tcgatccgtg
     6001 cgccatcgcg aggcgctgcg gccaccgacg ccgcggcttc ttcggggatc ccaaagccct
     6061 cgccgatcaa cgataccgtg atcttcgtgg cgcctccgga tcgtgaggcg cggctggaat
     6121 cgcgtgcccc ggttatcgtc aatccccgat ccagccagtt cacgaaggtc cagggcgtcg
     6181 atagcgttct ggcccgcctg cagggttcgc tcgatcagat cgaaggccgt cagatagcgg
     6241 cccttagctc ggtcgaagag agcatggaat cgcgcgttcg ccggatgcgc ggcgtgttca
     6301 ccgatctcgg c
//
LOCUS       DASWGM010000855         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1898495,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000855 DASWGM010000000
VERSION     DASWGM010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1898495"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1677)
                     /locus_tag="VGA15_23330"
     CDS             complement(301..1677)
                     /locus_tag="VGA15_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX9590661.1"
                     /translation="MTDDTPLPFDLPAVHRKKLTVDFDGGTQSSDAGLLLLREAERKL
                     GVCRRLAAAMPDRRDPDRILHAMFEMVMERVSAIACGYKDAIDLDRLRYDPLMKATVG
                     RCPETGAPLASQSTISRLENSPSKTDAARLGVAMIDQFCATVKPGRIEIFDIDDTFCA
                     AHGGQQLAFWNAHHDERGFASMHIYHVASGAPVATILRPARTPKGTEVRTVIKHVTKL
                     LKQHWPNTRIVWRGDSHYGRVEAMEWIEENDGDYIFGLPGNSTLDALVAKTADNLRFH
                     HAKSHQEKVRTHTSFTYQAGSWTCPRKVVARLECSLQPDAGETTSNGMRQEVDVRYVV
                     TSLKGPAEYLYEDVYCGRGQMENLIKLHKAQLASDRMSCHSATANQVRLALHTAAYWL
                     MLAVRDAIPQTDPLAKAEFATIRERLIKIGARVIEHLARIRIQLPTSCPEGRLFRAVA
                     LRLAPSTG"
BASE COUNT          327 a          592 c          594 g          374 t
ORIGIN      
        1 gaggccccga caatgacgag gccgccaaac tgaggcggtc tctaaggttt atctgtgggt
       61 taaacccgtc ggaagtaggc gccggggttc tggggaggcg atttcctttt gaagctctgc
      121 gtcgatcagc atttctccta gcccggatca tgcaccacac gccccatttt tgcacgtcgg
      181 cacggcgcac gcgcgccgcc ggccggtccg accggaccgg tttgccgcaa ctcgcaatgc
      241 aacgcgtttg gggttgatct gcctcgctcg gcggcgcatc ggggcacgac gccccgtcgc
      301 tcatccggtg gatggcgcga ggcggagcgc gacagcgcgg aacaagcgcc cctcggggca
      361 gctggtcggc aactggatgc ggatgcgcgc gaggtgttcg atcacgcgcg cgccgatctt
      421 gatcaggcgc tcgcggatag tggcgaactc agcctttgcg agcgggtctg tctgcggaat
      481 ggcgtcgcgt acggcgagca tcagccagta cgcggcggtg tgaagcgcga gccgcacctg
      541 attggcggtc gcgctgtgac acgacatgcg gtccgacgcc agttgcgcct tgtgcagctt
      601 gatcaggttc tccatctgcc cgcgcccgca gtagacgtcc tcataaagat actccgccgg
      661 gcctttgagc gaagtgacga cgtagcggac atccacctcc tggcgcatgc cgtttgacgt
      721 cgtctctccg gcatccggct gcaacgaaca ctccagccgc gccaccactt tgcgcggaca
      781 ggtccagctg cctgcctgat aagtgaagct cgtatgggtg cgcaccttct cctgatggct
      841 tttggcatga tggaagcgca gattgtcggc ggtctttgcc accagcgcat caagcgttga
      901 gttgccggga aggccaaaaa tgtagtcgcc gtcgttctct tctatccatt ccattgcctc
      961 gacgcggccg taatgactgt cgccgcgcca cacgatgcgg gtgttgggcc aatgctgctt
     1021 cagcagcttc gtcacgtgct tgatgacggt cctcacctcc gtgcccttcg gggtgcgcgc
     1081 cggtcgcagg atggtggcga ccggcgcgcc gctcgcgaca tgatagatgt gcatcgaagc
     1141 gaagccgcgc tcgtcgtgat gcgcattcca gaacgcaagc tgctggccgc catgcgccgc
     1201 gcagaacgtg tcgtcgatgt cgaagatctc aatccgcccc ggcttcaccg tcgcgcagaa
     1261 ctgatcgatc atggccacgc cgagccgcgc cgcgtcggtc ttgctcggcg aattctccag
     1321 ccgggagatg gtcgattgcg acgccagcgg cgcaccagtc tccggacatc gcccgaccgt
     1381 ggccttcatc aaaggatcgt accgcagccg gtcaaggtcg atcgcatcct tgtatccgca
     1441 cgcgatcgcg gacacgcgct ccatcaccat ctcgaacatg gcatgcagga tacggtccgg
     1501 atcgcggcga tccggcatcg ccgccgcaag ccgccggcac acaccaagct tgcgctccgc
     1561 ctcgcgcagt agcaacaaac cggcatcgga ggactgggta ccgccatcaa aatctactgt
     1621 gagtttcttg cggtggacgg ctggcaggtc gaaagggagc ggcgtatcgt cggtcatggc
     1681 ggctgtggcg ctgcgttgat tgcacgaggc agttcgattg ggcaccgatt tgctatgtga
     1741 atcggccgct tcgcgctaca gtcgccgcaa aatttctaca gctgatgcat aatccgggct
     1801 aggagcccgt ctagattatc tcggcgcatg aaggtaggcc aagctcttgc tgggattggc
     1861 tggtggcgtg acttgttttt cacgcca
//
LOCUS       DASWGM010000856         4841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2478740,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000856 DASWGM010000000
VERSION     DASWGM010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2478740"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VGA15_23335"
     CDS             complement(<1..323)
                     /locus_tag="VGA15_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX9590662.1"
                     /translation="MFGKRSGAENDSRAMKPAASVLEPPSGAPAAVSREPATPTVSAP
                     PLSPAKPAAAAPAIDARRSDNYYQVKATIFGALIEAIDLAQLAKLDGESAREEIRDIV
                     NEIIA"
     gene            complement(345..1613)
                     /locus_tag="VGA15_23340"
     CDS             complement(345..1613)
                     /locus_tag="VGA15_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9590663.1"
                     /translation="MISYARLNSEQQPDDTAPVADDHIAPAPRVSVQAFCETVETAAA
                     VQSAGEDRRLGKAHLKIQMGGMAAAIEAYRTAPTPNVIILETEGRTDILTGLDQLATV
                     CDSGTRVVVIGRVNDVGLYRELVRRGVSDYAIAPVNALDVVRSVCNLFSAPEAKAVGR
                     IIAIVGAKGGVGASTVAHNVAWAIARDLSLDSVVADLDLAFGTAGLDYNQDPPQGIAD
                     AVFSPDRVDTAFIDRLLSKCTDHLSLLAAPATLERVYDFGADAFDSIFDTLRVTMPCI
                     VLDVPHQWSGWTRRALIGADDILIVAAPDLANLRNTKNIFDLLKAARPNDRTPLYCLN
                     QVGVPKRPEINASEFAKAIESQPIAALPFDPQTFGSAANNGQMIAEISANHRTTEMFL
                     QIAQRLTGRGETKKPRGSFLSPLLEKLRAK"
     gene            complement(1610..2347)
                     /locus_tag="VGA15_23345"
     CDS             complement(1610..2347)
                     /locus_tag="VGA15_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEX9590664.1"
                     /translation="MTARIPVDRNRAFRMAGALVGLAVVLGACTHTDDVLTASVPDDF
                     HQRHPIAIQEADRSVVIFVGHARGGLSASQRADVMGLAQAWLHEGTGAINADVPVDTA
                     NARVAADSFREVRALLAASGVPPRGIVVRHYRPEDPRQMATIRLNYPKISAVAGPCGL
                     WPEDLGPSIKNKSYFENKDYYNFGCAYQRNMAAMVDNPSDLVQPRSETPAYTTRRTEA
                     FEKYRKGNPTTTTYPEAERAKLSDTGK"
     gene            complement(2369..3850)
                     /locus_tag="VGA15_23350"
     CDS             complement(2369..3850)
                     /locus_tag="VGA15_23350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX9590665.1"
                     /translation="MKCRGNQLVMRTLLIRALSFSAVTALTLNPALTPVVASDYRVAA
                     PLAADGQMNARFLALGIGKSVVIDLPRDIKDVLVADPKIANAVVRSAQRAYIIGVAVG
                     QTNIVFFDSAGQQIAAYDIAVKRDLNGVRAALKQSLPNAEIQIEGIGDGVVLSGSAAS
                     PIEAQQAGELAARLVGGNDKVVNSIVVRGRDQVMLKVTVAEVARSVIKQMGIDLTATM
                     NYGTAAVTFDNSNPFTALGHTLTGNSVAGAFGSAMRGTTPVPSVQATIRAMESAGVIR
                     TLAEPNLTAISGESATFIAGGEFPIPAGYSCDPVTHVCQTQISFKKFGISLNFTPIVM
                     TEGRISLRVMTEVSELSNDNSFTLVQSAGNALTIPSIKTRRAETTLEIPSGGAMAMAG
                     LIQEQTKLAINGLPGLAQLPVLGALFRSRDFVNNQTELMVLVTPYIVRAVAQKDLSRP
                     DDGFANASDPQADLIGNINRIYGVPGRVEPARNYRGTYGFITD"
     gene            complement(3868..4656)
                     /gene="cpaB"
                     /locus_tag="VGA15_23355"
     CDS             complement(3868..4656)
                     /gene="cpaB"
                     /locus_tag="VGA15_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX9590666.1"
                     /translation="MNKARIVVLSIAVGAGGIAAYLASGSDSKPPSEPVAQLQTVDVL
                     VAKSDIGLGQSVASDDLLWQTWPAASASNSFIRRSERPDATKEVTGSIARAPFIAGEP
                     IREPKLVKANGSGFMAAILPTGMRAISTEISPETGAGGFILPNDRVDVILSKRDKNPD
                     KSGSADIVVSEIILTNVRVLAIDQAPKEKDGQNAVVGKTVTLELKPEQAETLARARQT
                     GTLALALRSIADLNAVENRSSDPRQSDNINVVRYGVSSPTTMQK"
BASE COUNT          858 a         1487 c         1558 g          938 t
ORIGIN      
        1 atcgcgatga tctcattgac gatatcgcga atttcttctc gcgccgactc gccgtcgagc
       61 ttggcgagct gggcgaggtc gatcgcttcg atcagggcgc cgaagatggt cgccttgacc
      121 tgataatagt tgtccgaccg ccgcgcgtcg atggcgggcg cggccgccgc cggctttgcg
      181 ggcgagagcg gcggcgcgga aacggtaggc gttgctggct cacgcgaaac ggccgccggc
      241 gcgccggaag gcggctccag cacactggcg gcgggtttca tcgcccgcga atcgttttca
      301 gctccgctac gcttaccaaa cacgacggca ctccatacgg gcggttactt ggcccgcagc
      361 ttttcgagga ggggcgacag gaacgaaccc cgtggctttt tggtttcgcc acggcctgtc
      421 agccgttgcg cgatctgcag aaacatctcg gtggtgcgat gattggcgga gatttccgcg
      481 atcatctggc cgttattcgc cgccgagccg aatgtttgcg ggtcgaacgg aagcgccgcg
      541 atcggctggc tttcaatggc cttggcgaat tcgctggcgt tgatttcggg ccgcttcggc
      601 acgccgacct ggttgagaca atagagcggc gtccggtcgt tgggccgcgc tgccttcagc
      661 aggtcgaata tgttcttggt gttgcgcagg ttggcgagat caggtgctgc gacgatcaga
      721 atatcgtccg caccgatcag cgcgcgcctg gtccatccgg accattggtg aggaacgtcg
      781 agcacgatgc acggcatcgt gacgcgaagc gtgtcgaaga tcgagtcgaa ggcatcggcg
      841 ccaaaatcgt agacccgctc gagcgtcgcc ggcgccgcca gcaggctgag gtggtcggtg
      901 catttcgaca gcaggcggtc aatgaacgcg gtatcgacgc gatcgggcga gaacaccgca
      961 tccgcgatcc cttgcggcgg atcctgattg tagtcgaggc cggcggtgcc gaacgcgagg
     1021 tcgagatctg cgacgaccga gtccagcgaa agatcgcgcg cgatcgccca ggcgacgttg
     1081 tgcgcgacgg tggatgcgcc gacgccgcct ttggcgccga cgattgcgat gatccggcca
     1141 accgccttcg cctccggcgc cgagaacaga ttgcaaaccg aacgcacgac gtccagcgcg
     1201 ttgaccggcg cgatcgcata gtcgctgacg ccacggcgca ccaattcgcg ataaagcccg
     1261 acatcgttga cacggccgat cacgacgacg cgggttccgg aatcgcacac ggtcgcgagc
     1321 tgatcgagac cagtcaggat gtcggtgcgg ccctcggttt ccaggatgat cacgttcggt
     1381 gtaggcgcgg tgcgataggc ttcgatggcg gctgccatgc cgcccatctg aatcttgaga
     1441 tgggccttgc caagccgacg gtcctcgccc gccgattgca cggcggccgc ggtttccacc
     1501 gtctcgcaaa aggcttgcac agacacacgc ggcgcaggcg cgatgtgatc gtccgcgacg
     1561 ggcgctgtgt cgtctggctg ctgttcggaa ttcagacgcg cgtagctgat catttgcctg
     1621 tatcactcag tttggcccgc tcggcctcgg gataggtggt cgtggtcggg ttacccttgc
     1681 gatacttctc gaatgcttcc gtgcgtcgcg tcgtatacgc aggcgtctcg gaccgcggtt
     1741 gcacgaggtc cgacggattg tcgaccatcg ccgccatgtt gcgctgatag gcgcagccga
     1801 aattgtagta atccttgttt tcgaaatagc tcttgttctt gatcgagggt ccgagatcct
     1861 ccggccacag gccgcagggt cccgcgaccg ccgatatttt cgggtaattc agccggatcg
     1921 ttgccatctg gcgcgggtct tccggacgat agtggcggac aacaatcccg cgcggcggca
     1981 cgccacttgc ggcgagcagc gcccgaacct cccggaacga atccgcggcc acccgcgcgt
     2041 tcgccgtgtc gaccggcacg tcggcgttga tcgcacctgt gccttcgtgg agccaggcct
     2101 gcgccagccc catcacgtcg gcgcgctgcg aagcggaaag accgccacgc gcatgaccga
     2161 cgaaaatgac gactgaccgg tcggcctcct gaatggcgat cggatgacgc tggtgaaagt
     2221 cgtcaggaac gctcgccgtc agcacgtcgt cggtgtgcgt gcaggctccc agcacgacgg
     2281 cgagaccaac aagcgctccg gccatgcgga aggcgcgatt gcggtcgacg ggtattctgg
     2341 ctgtcatcgt tttggtcctc gtcccgtctc aatcggtgat aaagccgtag gtgccgcgat
     2401 aattccgtgc cggctcgacg cggcccggaa ccccgtagat gcgattgatg ttgcctatga
     2461 gatcggcttg cggatccgac gcattggcaa atccatcatc cggacgcgac agatccttct
     2521 gcgcaaccgc ccggacgatg tagggcgtca cgagcaccat cagctcggtc tggttgttga
     2581 cgaaatcgcg gctgcggaac agcgccccga gaaccggcaa ttgcgccagg ccgggcaggc
     2641 cgttgatggc cagtttggtc tgttcctgga tcaggcccgc catcgccatc gcaccgcccg
     2701 acggaatttc cagcgtggtt tcggcgcgcc gggtcttgat cgaggggatc gtcagcgcat
     2761 tgccggccga ctgcaccagc gtaaaggaat tgtcattcga taactccgac acctcggtca
     2821 tcacgcgcag gctgatccgg ccttcggtca tcacgatcgg cgtgaagttg agagagatgc
     2881 cgaacttctt gaagctgatc tgggtttgac aaacatgcgt gacgggatcg caggaataac
     2941 ctgccggtat cggaaactcg ccaccggcaa tgaacgtcgc cgactcgccg gaaatcgccg
     3001 tcaggttggg ttcggccagc gtgcggatca ccccggcgct ttccatggcg cgtatggtgg
     3061 cctgcaccga gggcacagga gtagtgccgc gcatcgccga accaaaagcc cctgccaccg
     3121 aattgccggt gagggtatgg ccgagtgccg tgaacggatt gctgttgtcg aacgtgacgg
     3181 cggcggtgcc gtagttcatg gtggcggtga ggtcgatgcc catttgtttg atgaccgaac
     3241 gcgcgacctc ggcgacggtg accttcaaca tgacctgatc gcggccacgg accacgatgg
     3301 agttgacgac cttgtcgttg ccgcccacca gtcgggcggc aagttcgccc gcctgttgcg
     3361 cttcgatcgg actcgccgcc gagccggaca gcaccacgcc gtcgccgatg ccttcgatct
     3421 gaatctcggc attcggcagc gactgcttca gcgcggcgcg tacgccgttg agatcgcgtt
     3481 tgaccgcgat gtcgtaagcc gcgatctgct ggcctgccga gtcgaaaaac acgatgttgg
     3541 tctggccgac cgccacgccg atgatgtagg cgcgctgcgc ggaacgcacg acggcgttgg
     3601 caatcttcgg atcggcgacc agcacatcct tgatgtcacg cggcaaatcg atcacgaccg
     3661 atttgccgat gccaagcgca agaaagcgcg cgttcatctg accgtcggca gcaagcggcg
     3721 ccgcgacgcg atagtcgctt gccacgaccg gcgtcagcgc cgggttgagt gtcagcgcgg
     3781 tgacggccga aaacgacagg gcgcggatca aaagagtccg catcaccagc tgatttcccc
     3841 tgcacttcat atcgggcgtc cttcttatca cttctgcatc gtcgtcgggc tggagacgcc
     3901 gtagcgaacc acgttgatat tgtcgctctg cctgggatcg ctggatctgt tctcgaccgc
     3961 gttgagatcg gcaatgctgc gcagcgccag cgccagcgtg ccggtctggc gcgcgcgggc
     4021 aagcgtctcg gcctgctcgg gctttaattc aagcgtgacg gtcttgccga ccacggcatt
     4081 ctggccgtct ttttccttcg gagcctggtc gatcgcgagc acgcgaacat tggtcaggat
     4141 gatttccgag acgacgatat ccgctgaacc cgatttgtcc gggttcttgt cgcgcttcga
     4201 aagaatcaca tcgacccggt cgttcggcag gatgaagccg ccggcgccgg tctccggcga
     4261 gatttcggtc gagatggcgc gcatgccggt gggcaggatc gccgccatga agcccgatcc
     4321 gttggccttg acgagtttcg gttcgcggat cggctcgcct gcgataaacg gcgcgcgcgc
     4381 gatcgagccg gtgacttcct tggtggcgtc aggccgttcg ctgcggcgaa tgaaactgtt
     4441 gcttgcactc gccgccggcc aagtctgcca cagcaggtca tcggacgcga ccgactggcc
     4501 gaggccgatg tccgacttcg ccaccagaac gtccaccgtc tgcagttgcg caaccggttc
     4561 ggacgggggc ttgctgtcgg agccactcgc aagatatgcg gcaatgccgc cggcaccgac
     4621 ggcgatgctc aggacgacaa tgcgtgcctt attcatacgc ttcactttcc acgttacgcc
     4681 gcgacgcttc cgccgccatg acgggagttg agcagttatt cgtcaaagca tggttaatga
     4741 ggcgtatcta aatcgcctta acggcgagtt acttgaggac gcggtcagcg aacggcgaag
     4801 tgggtcagat cgaccgcgtt gatccattcc gtctccggat a
//
LOCUS       DASWGM010000857         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1465048,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000857 DASWGM010000000
VERSION     DASWGM010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465048"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VGA15_23360"
     CDS             complement(<1..140)
                     /locus_tag="VGA15_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX9590667.1"
                     /translation="MNHDAYADNYIREILSGVKSIAMVGASPVNVRPSYFAFKYLAQR
                     GY"
     gene            complement(288..1112)
                     /locus_tag="VGA15_23365"
     CDS             complement(288..1112)
                     /locus_tag="VGA15_23365"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9590668.1"
                     /translation="MTSQTARAAIPQTPVLLRETLGSIAVLTLNRPDARNSLSEGLIG
                     ELHAALKDVCDDTNVRAVVIAANGPAFSSGHDMKELTARRTDADRGRAYFAGIMTACS
                     AMMQAIVHLPKPVVAAVQGIATAAGCQLVASCDLAVASEAATFATPGVDIGLFCSTPM
                     VALSRNVPRKQAMEMLLTGEPISAARARDIGLINRVVAAGTERDAAIALAQQVALKSA
                     HTIKLGKAAFYRQAEMSLADAYRYAAEVMTENMMARDAEEGIGAFIEKRAPKWQDR"
     gene            complement(1281..1916)
                     /locus_tag="VGA15_23370"
     CDS             complement(1281..1916)
                     /locus_tag="VGA15_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9590669.1"
                     /translation="MAKEQRIGRQKAVSELKRGLIMDAARQVFEAEGLDGASLRAIAT
                     AAGYTPAALYFHFESKEAIYAEVLRESLADLRKAVVRAVTRAKTPADRFRAAAMAFFR
                     YYADNPRDLDLGFYLFRGGMKPHGLGRERDEVLNTALELAMHPIADAAQALGARHEET
                     RLLMADIFAHAAGLLLLAHTGRIRMFGASAPHLMERFVDSATLALAEKKKR"
     gene            2008..2823
                     /locus_tag="VGA15_23375"
     CDS             2008..2823
                     /locus_tag="VGA15_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9590670.1"
                     /translation="MTVRIEKDGSVWTIIHDRPEARNAMDPQSADALTAAFLEFDADR
                     DAAVAVFYGAGGAFCSGWDLKYVSTLNAAYPLGELDISTARPRGNGGEIPRGPLGPSR
                     LELDKPVIAAIEGPAVAGGMELGLWCDFRVMAEESYFGVYCRRWGVPLIDGGTVRLPR
                     IVGQGRALEIILTGRKVPAEECMSIGLCEYLTPKGGARAKAEALAQEIARFPQVCVRA
                     DRRSAIKSHGLTVREALIQEWYNGREALIADGVAGAGRFSEGLGRHGDFARIR"
     gene            2848..3276
                     /locus_tag="VGA15_23380"
     CDS             2848..3276
                     /locus_tag="VGA15_23380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX9590671.1"
                     /translation="MTAARMTAAELGAFLRTELPLMFDYDDLTIEHADGRTCQVRQRF
                     RENMLRPGGTVSGATLMALADCAMYVVLLSAIGPISLAVTTNLNINFLRKGAPGQDLV
                     ATAKLLKLGKRLAVGEVTLLPGASVDPIAHVTATYSIPNT"
BASE COUNT          595 a          983 c         1071 g          702 t
ORIGIN      
        1 tcgtagccgc gctgcgccag atatttgaaa gcaaagtagc tcggccgcac attgaccggc
       61 gacgccccga ccatcgcgat cgatttcacg ccgctcagaa tttcgcggat gtaattgtcg
      121 gcgtaggcgt catggttcat ctgatcgatc attcattatg gacgtcattg cctagggcga
      181 agcgacgaag caaatccaga cttcgtcatt ccgggatggt gctttagcac cagacccgga
      241 atctcgagat tccgggttcg atgcttcgca tcgccccgga atgacggtta cctatcttgc
      301 catttcggcg cgcgcttctc gatgaaggcg ccgatgcctt cctcggcgtc gcgggccatc
      361 atgttctcgg tcatcacctc tgccgcatag cgataggcgt ccgcaaggct catctcggcc
      421 tgccggtaga atgcggcctt gccgagtttg atcgtatggg cggatttcag cgcgacctgc
      481 tgcgccagcg cgatcgcggc atcccgctcg gtgccggctg cgaccacgcg gttgatcagg
      541 ccgatatcgc gcgcccgcgc tgccgaaatc ggctcgccgg tgagcagcat ttccatcgcc
      601 tgtttgcgcg gcacattgcg cgacagcgcc accatcggcg tcgagcagaa caatccgata
      661 tcgacgccgg gggtagcgaa cgtcgcggcc tccgatgcca ccgcaagatc gcagcttgct
      721 accaactggc atcccgccgc ggtggcgata ccctggaccg ctgccaccac cggctttggc
      781 aagtgaacga tggcctgcat catggcgctg caggcggtca tgatcccggc gaaataggcg
      841 cgtccgcgat cggcatcggt gcggcgggcg gtgagctcct tcatgtcatg gccggacgag
      901 aacgccggac cgttggccgc gatcacgacc gcgcgcacgt tcgtgtcgtc gcaaacgtct
      961 ttcagcgccg cgtgcagttc gccgatcaat ccttccgaca ggctgttgcg ggcatcaggc
     1021 cgattgagcg tgagcacggc gatgctgccc agtgtctcgc gcaggagaac cggcgtttgc
     1081 ggtatggcgg cgcgtgcggt ctggctggtc atcgaatttt ccaatagctg gtcggtcata
     1141 cggatattgt atgcactcgt ccaccgggcg ccagtcggcg catgtccacg cgttcaccaa
     1201 aattgagggg tggaatgcgg ttttcaagtc cggctttggt aggatcgtgg agcagccccc
     1261 ggggcgcggg ctgccatcca ttatctcttt ttcttctcgg ccagagccaa agtcgcgctg
     1321 tccacaaatc gttccatgag gtggggcgcc gaggcaccga acatgcggat gcgcccggtg
     1381 tgggccagga gcagcagccc cgccgcatgc gcgaagatgt ccgccatcaa cagtcttgtc
     1441 tcctcgtgac gggcaccgag cgcctgggcg gcatcggcga tcggatgcat ggccagttca
     1501 agggccgtat tgaggacttc atcgcgttcc ctgccgagac cgtgcggctt catgccgccg
     1561 cgaaacagat agaagccgag atcgaggtct ctggggttgt ctgcgtaata ccggaagaac
     1621 gccatggcgg ctgcccggaa ccggtccgcc ggtgttttgg cgcgcgttac ggcgcgaacg
     1681 acggcctttc tcagatcggc cagcgattcg cgaagaacct ccgcgtatat cgcttccttg
     1741 gattcgaagt gaaaataaag cgccgcgggc gtgtagccgg cggccgtcgc gatcgcccgc
     1801 aatgaagcgc cgtccagccc ttcagcctcg aacacctgcc gtgcggcgtc catgatcaag
     1861 ccgcgcttca gctcgctcac cgccttctgc cggccgattc tctgttcttt tgccatcggt
     1921 cgttttatta gcacacttga cggtttattt tacactgtta gaatttttaa catcgttaga
     1981 attaatgacc ggcaacagga ggctccgatg acagtgagga tcgagaagga cggctcggtt
     2041 tggaccatca tccacgatcg gcccgaagcc cgtaacgcca tggatcccca aagcgcggac
     2101 gccctgaccg cggccttcct ggagttcgat gccgacaggg atgctgcggt tgcggtattt
     2161 tatggggcag gcggcgcgtt ctgctccggc tgggatctga aatatgtcag cacgctcaat
     2221 gccgcctatc cgctgggcga gcttgatatt tcgacggcgc ggccgcgcgg caatggcggc
     2281 gaaattccac gcggcccgct gggaccgtct cgtctggaat tggacaaacc ggtgatcgcg
     2341 gcaattgagg ggccggcggt cgcgggcgga atggaactgg gattgtggtg cgattttcgg
     2401 gtgatggccg aggagtcgta ttttggcgtt tattgccgcc gatggggcgt gccgctgatc
     2461 gatggaggca cggtgcggtt gccgcgcatt gtgggacagg gccgggcgct cgagattatc
     2521 cttaccggcc gcaaagtgcc ggccgaggag tgcatgtcca tcggcttgtg cgaatacctg
     2581 acgccgaagg gcggagcgcg cgcgaaggcg gaggcactcg cacaggagat cgcgcggttt
     2641 ccgcaggtct gtgtccgtgc cgaccgacgg tctgccatca agagccatgg ccttactgtc
     2701 cgcgaggcgc tgattcagga atggtacaac gggcgcgagg cgttgatcgc agacggcgtg
     2761 gcgggggcag ggcgcttcag cgaggggctc ggccggcacg gcgattttgc gagaattcgc
     2821 tagcgcttcg gccgtctggt ttcagcaatg acagcggcgc gaatgacggc ggcggaactc
     2881 ggtgccttcc tgcgcaccga gcttccgctt atgtttgatt atgacgatct tacaatcgag
     2941 cacgccgacg gtcggacctg ccaggtaagg cagcgcttcc gtgaaaatat gcttcgtccc
     3001 ggcggaaccg tctcgggagc tacgctgatg gcgctggcgg attgcgcgat gtatgtggtg
     3061 ctgctgtcgg cgattggccc catcagcctt gcggtcacga ccaatctcaa tatcaatttt
     3121 ctgcgcaagg gagcgcctgg gcaggatctc gtagcgacag cgaagctttt gaagctgggc
     3181 aagcggctcg ctgttggcga ggttacgctg ctgccagggg cctcggtcga tccgatcgcc
     3241 catgtgaccg cgacctattc cattccaaat acttagtgtt ttgtgcggta atatagcacc
     3301 atatttgcaa gtcattgatt tagtgcagtt aattaacgtc gatcggcgtt g
//
LOCUS       DASWGM010000858         6958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2184916,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000858 DASWGM010000000
VERSION     DASWGM010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6958
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2184916"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VGA15_23385"
     CDS             <1..149
                     /locus_tag="VGA15_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9590672.1"
                     /translation="ANRSSFAVGPITDDIVATQQGVADRFFKLGLIPKPVEVYDAVWK
                     SVQT"
     gene            187..1137
                     /locus_tag="VGA15_23390"
     CDS             187..1137
                     /locus_tag="VGA15_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9590673.1"
                     /translation="MRWFKRAVAIAVLSMTTALAGVGASYGQDKVVRIGFQKYGKLVL
                     LKGKGSLEEKLKAAGYKVVWTEFPSGPPLLEALNVGAIDFGNTGEAPPIFAQAAGAPI
                     QYVAYEPPAPKGEAILVPKDSKLTSVADLKGKKIALNKGSNVHYLLVKALEKAGVKYS
                     EVEPVFLAPADARAAFERGAVDAWVIWDPFQAAAEAATGARTLADGTGIVSNYQFYFS
                     SKKFLESDPRIVDLILAQLSEVDDWAKGDIQAVADQLAPTIGLPVGVVEIALKRQAYG
                     IKPITDSVIADQQQVADTFFALGLLPKQIKISDVARRPGT"
     gene            1134..2306
                     /gene="ssuD"
                     /locus_tag="VGA15_23395"
     CDS             1134..2306
                     /gene="ssuD"
                     /locus_tag="VGA15_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HEX9590674.1"
                     /translation="MSINQSSKANILWFLPTHGDGRYLGTTTGGREVNFNYLRQIAQA
                     ADQLGYFGVLLPTGRSCEDSWVVASAVAPWTERLRYLVAVRPGLQSPSVAARMTATLD
                     RVSNGRLLINVVTGGDPVENKGDGIFLDHDARYAVTREFLNIYSDLLAGKTVNVEGEH
                     IRIEDGRLLFPPVQSPRPPLYFGGSSDAGIDVAVDTVDKYLTWGEPPAQVAEKVARVK
                     AVADKRGRKLSFGIRLHVIVRETSAEAWKAADELIQHVTDETVASAQKIFSRMDSVGQ
                     QRMAQLHGGRRDKLEISPNLWAGVGLVRGGAGTALVGDPQTVAARIREYQEVGIDTFI
                     MSGYPHLEEAYRFAELVFPLLSLAQPNNVTPLRVNTGPFGETIANEHRPLVKASQS"
     gene            2303..3109
                     /locus_tag="VGA15_23400"
     CDS             2303..3109
                     /locus_tag="VGA15_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9590675.1"
                     /translation="MSLIESLPRVRSLRLPRADGLIQWIVPLVIVIVWQIACVSGLVP
                     ARVLPAPSDVALAGWKLLLSGELVRNIWVSFWRASVGFVIGGGIGFAFGLMNGLSQLS
                     NKLTDTTLQMVRNIPHLALIPLVILWFGIDESAKLFLVALGVFFPIYLNTLHGIRTVD
                     PQLIEMGRIYGMTDGELFRRVIFPGALPSIFVGVRFALGIMWLTLIVAETIAASSGLG
                     YMAMQAREFMQIDVVVLSILIYALLGKLADSASRVLERLTLSWHPAFQKH"
     gene            3118..3960
                     /locus_tag="VGA15_23405"
     CDS             3118..3960
                     /locus_tag="VGA15_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9590676.1"
                     /translation="MQEALRFLPPDAEPVDRADFVGRARLLRRDSEVSRGLPLTIRGL
                     RKSFGDNEVLRGIDLHIPAAQFVAIVGRSGCGKSTLLRLIAGLETIDAGTIGFGEETR
                     PEDVRVMFQEPRLLPWARVLSNVEVGLGRDRASSDAQIRAQKALTEVGLDDKRSQWPA
                     VLSGGQKQRVALARALVSHPRVLAFDEPLGALDALTRISMQRLLERVWRDQAFTAILV
                     THDVSEAVALADRVLVIENGRIAHDIDVDIPRPRQRGSADLAALEGSILRGLLQSADD
                     PSDL"
     gene            3968..4552
                     /locus_tag="VGA15_23410"
     CDS             3968..4552
                     /locus_tag="VGA15_23410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9590677.1"
                     /translation="MNSTVRTITIDREVSSGDFRSAMRHLAGGVSVITVGRGKEITGM
                     TVTSASSLSVEPPTLIVSINRGSSSWPLLKRLGVFGVNILTADQLDIAERFTGKDGLK
                     GADRFAGAEWVTRVSGVLLLVGALAAVDCEVEDIVERHSHVIVIGRVLDLQVSKRTAA
                     LAYWQGQYVAIDQNEDVVKLAEVSLPARRIPRMT"
     gene            complement(4574..5929)
                     /locus_tag="VGA15_23415"
     CDS             complement(4574..5929)
                     /locus_tag="VGA15_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX9590678.1"
                     /translation="MQPNSDISFEAALDERVADMLDACTRCGKCVEVCPSVKPAGIAE
                     ASSEDIIGGVLDIVRSGDGPEASRRWAASCMLSGECIEACDYGVNPRFLLAMARLAIA
                     KAGSELPDRRRLGVERYRDLSRDVTVLSRLQLDGEVLERLGQKSASVSTPAEAPDFVF
                     YTGCNVLKTPHIALLALDIMDVLGVTYQVMGGPSHCCGVVQLRPGDIEMSGRMGSNSI
                     EKLSHSKSGQVISWCPSCYVQFTELTLPTIERQRGSRPFEMTPFMRFLDGRLAQLIPH
                     LRRRVEMRVAMHQHPGVAGAMEAAAEILQAIPGVELVDLKQPAVGLQSASLGVLPAFK
                     RELQLGELQAARDAGVDALITVYHSEHRELCAHERDWPFRIINILEVVGESMGLHRED
                     RHKQLKIMQDADQIVSECGDLIARHALDAGKARDVVIKAMLGDQPLPLSRGSQALGAP
                     T"
     gene            6172..6567
                     /locus_tag="VGA15_23420"
     CDS             6172..6567
                     /locus_tag="VGA15_23420"
                     /inference="COORDINATES: protein motif:HMM:NF025607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HEX9590679.1"
                     /translation="MERMLLRIAVAVAGLAGVGLGLTGVVFGTLDADLSGDVVLDSYV
                     RFLKGMLLAMGLIYWSCIPQIDRYGDRISLVTFILVAGTLARLMSVIGHGVPTVGIMI
                     NVIAGLVFVPLLWLWQRRVARVAQRGAVT"
     gene            6567..6722
                     /locus_tag="VGA15_23425"
     CDS             6567..6722
                     /locus_tag="VGA15_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590680.1"
                     /translation="MRRIAVWIFYAVGALAILYLALYAYAAFTGHDLQPGDPIHIFRK
                     PDAPSYS"
BASE COUNT         1195 a         2140 c         2262 g         1361 t
ORIGIN      
        1 ccgccaaccg ctcgtccttt gcggtcggcc cgatcaccga cgatatcgtt gccacccagc
       61 aaggtgtggc cgatcgcttc ttcaagttgg gtctcattcc aaagccggtc gaggtttacg
      121 acgcggtctg gaagtcggtg cagacctgag ctgaccctca caacatccgt cacagaggat
      181 cgaaacatgc gttggtttaa acgagcggtc gccattgcgg tgctgtcgat gaccacggcg
      241 ctggccggag ttggtgccag ttacggccag gacaaggtcg ttcgcatcgg ctttcaaaaa
      301 tacggcaagc tggtgttgct caaaggcaag ggctcgcttg aggaaaagtt gaaggcggcg
      361 ggttataagg tggtgtggac ggagtttccg tccggtccgc cgctgctcga agcgctcaat
      421 gtcggcgcga ttgatttcgg caacaccggc gaggcgccgc cgatcttcgc gcaggccgcg
      481 ggcgcaccga tccagtatgt cgcctatgag cctcccgcgc cgaaaggcga ggctattctt
      541 gtgccgaaag acagcaagct gacttcggtg gccgatttga aaggcaagaa gatcgcgctc
      601 aacaagggct ccaatgttca ctatctcttg gtgaaggcgc tggaaaaggc cggcgtcaaa
      661 tattcggaag tcgagccagt gtttctcgcg ccggctgacg cccgcgcagc attcgagcgc
      721 ggcgcggtcg atgcctgggt gatctgggat ccattccagg ctgccgccga ggccgccacc
      781 ggcgcgcgga cgctggcgga tggcaccggc atcgtgtcga actatcaatt ctatttctct
      841 tcgaagaaat tcctcgagag cgatcccagg attgtcgatc tgatactcgc ccaactgagc
      901 gaggtcgatg attgggccaa gggcgatatc caagcggtcg ccgaccagct cgcgccgaca
      961 atcgggcttc cggttggggt tgtcgagatc gccctgaagc gacaggccta tggcatcaaa
     1021 ccgatcaccg acagcgtgat cgcggatcag cagcaggtcg cggacacgtt tttcgcgctc
     1081 ggcctgctgc caaaacaaat caagatttcc gacgtggcgc ggaggcccgg cacgtgagca
     1141 tcaatcagtc atcgaaggcc aacatcctct ggttcctgcc gacccatggc gacggccgct
     1201 atctcggcac cacgaccggc gggcgcgagg tcaacttcaa ctatctgcgc cagatcgcgc
     1261 aggccgccga tcaactcggc tatttcggcg tgctgctgcc gaccggtcgg agctgcgagg
     1321 attcctgggt ggtggcgtcg gcggtcgcgc cctggaccga gcggctgcgt tatctggtgg
     1381 cggtgcgccc cggcctgcaa tcgcccagcg tcgccgcgcg catgaccgcc acactcgacc
     1441 gggtgtcgaa cggacgcctg ctgatcaatg tcgtcaccgg gggcgatccc gtcgagaaca
     1501 agggcgacgg catcttcctc gatcatgacg cgcgttatgc cgtgacccgc gagtttctca
     1561 acatctacag cgatctgctc gccggcaaga ccgtcaatgt cgagggcgag cacatccgca
     1621 tcgaggacgg acggctgttg tttccgccgg tgcaatcgcc gcgcccgccg ctgtatttcg
     1681 gcggatcgtc ggacgctggc atcgatgtcg cggtcgacac cgtcgacaaa tacctgacat
     1741 ggggcgagcc gccggcgcag gtggccgaaa aggtcgcgcg cgtgaaggcc gtcgccgaca
     1801 agcgcgggcg caagctctct tttggcatcc gcctgcacgt gatcgttcgc gaaaccagcg
     1861 cggaagcctg gaaagccgcc gacgaattga ttcagcacgt caccgacgag accgttgcct
     1921 ccgcacagaa gatcttttcg cggatggact cggtcggcca gcagcgcatg gcgcaactgc
     1981 acggcggacg ccgggacaag ctcgagatca gcccgaacct ctgggccggc gttggcctcg
     2041 tgcgcggcgg cgcgggaacg gcactggtcg gcgatcccca aaccgtggcg gcgcggatcc
     2101 gggaatatca ggaggtcggc atcgatacgt tcatcatgtc aggctatccg catctcgagg
     2161 aagcctatcg cttcgccgag ctggtgttcc cgttactgtc gctggcgcaa ccgaacaatg
     2221 tgacgccgct gcgcgtcaat accgggccgt tcggcgagac catcgccaac gagcaccgtc
     2281 cgctggtgaa ggcgtcgcag tcatgagcct gatcgaaagc cttccgcggg tgcgcagtct
     2341 gcgattgccg cgtgccgatg gcctgatcca gtggatcgtg ccgctggtca tcgttattgt
     2401 ctggcagatt gcctgcgtaa gcgggctggt gccggcgcgg gtactgccgg cgccgagcga
     2461 cgtggcgctg gcgggatgga agctgctgct gtcgggcgaa ctcgtgcgca acatctgggt
     2521 cagcttctgg cgggccagcg tcggctttgt cattggcggt ggcatcggct ttgccttcgg
     2581 cctgatgaac ggcctgtcgc agctcagcaa caagctcacc gacaccacgc tgcagatggt
     2641 gcgcaacatt ccgcatctcg cgctgatccc gctggtcatc ctgtggttcg gcatcgacga
     2701 atcggcaaaa ctgtttctgg tcgcgctcgg cgtgttcttc ccgatctatc tcaacacgct
     2761 gcacggcatc cgcaccgtcg atccgcaatt gatcgagatg gggcggatct atggaatgac
     2821 cgatggcgaa ttgttccggc gggtaatctt tccgggcgcg ttgccgtcga tctttgtcgg
     2881 cgtgcgcttt gcgctcggca tcatgtggct gacgctgatc gtggcggaaa ccatcgcggc
     2941 ttcgtcgggc ctcggctaca tggcgatgca ggcgcgcgaa ttcatgcaga tcgacgtcgt
     3001 cgtgctcagc atcctgatct acgctttgct tggaaagctg gccgacagcg cctcgcgcgt
     3061 gctggaacgg ctgacgctgt cctggcatcc cgcgttccag aaacattgag agtacgcatg
     3121 caagaagccc ttcgttttct ccccccggat gccgagccgg tggatcgcgc ggatttcgtc
     3181 gggcgggcgc ggctgctgcg gcgcgattcg gaagtctcac gcgggctgcc gctgaccatt
     3241 cggggcttgc gaaaatcgtt cggcgacaac gaggtgctgc gcggcatcga cctgcacatt
     3301 cccgcagcac aattcgtcgc gatcgtcggc cgcagcggct gcggcaagag cacgctgctg
     3361 cgtttgatcg ccgggttgga gaccatcgac gccggcacca tcggcttcgg cgaggaaacc
     3421 cggcccgagg atgtgcgggt gatgtttcag gagccacggc tcttgccgtg ggcgcgggtt
     3481 ctgtcgaacg tcgaggttgg gcttggccgc gaccgcgcat cgtcggatgc ccaaatacgc
     3541 gcgcaaaagg cgctgaccga agtcgggctt gacgacaagc gcagtcagtg gccggccgtc
     3601 ctctccggcg ggcaaaagca gcgcgtggcg ctggcgcgtg cgctggtcag ccatccccgc
     3661 gttctcgcct tcgacgagcc gctcggcgcg ctcgatgccc tgacgcggat ttccatgcag
     3721 cggctgttgg agcgggtctg gcgcgatcag gcttttacgg cgatccttgt cacgcatgac
     3781 gtgtccgaag ccgtcgcatt ggccgaccgg gttttggtca ttgaaaatgg ccgtatcgcg
     3841 catgacatcg atgtcgatat cccgcgtccc cgccagcgcg gctcggccga cctcgcggca
     3901 ttggaaggct cgatcttgcg cggtcttctg caatccgcgg acgacccatc agacctgtga
     3961 ggctgccatg aactccaccg tccgcaccat cacgatcgac cgcgaggttt cttcaggcga
     4021 ttttcgcagc gcgatgcgtc atctggcggg cggcgtcagc gtgatcacgg ttgggcgcgg
     4081 caaggagatc acgggcatga ccgtcacatc ggcatcgtcg ctgtcggtcg agccgccgac
     4141 attgatcgtc agcatcaatc ggggatcgtc gtcctggccg ctcctgaagc gccttggcgt
     4201 tttcggcgtg aacattctga ccgccgatca acttgacatc gccgagcgat ttaccggcaa
     4261 ggacggattg aagggcgcgg accggttcgc cggcgccgaa tgggtgacgc gagtgtcggg
     4321 cgtgctgctg ctggtgggcg ccctcgcggc ggtcgactgc gaggttgagg atatcgtcga
     4381 gcgtcactcc catgtcatcg tgatcggtcg cgtcctggat ttgcaggtct cgaagcgcac
     4441 agcggcgctg gcctactggc agggccaata tgtcgcgatc gatcagaacg aggatgtggt
     4501 caagctcgcg gaggtcagcc ttccggccag gcggatcccg cgcatgactt gatctggtga
     4561 ttcgcggccg tcgctacgtc ggtgctccca gggcctgcga gccacgactg aggggaagcg
     4621 gctgatcgcc gagcatcgcc ttgataacga cgtcgcgggc tttcccggcg tcgagcgcat
     4681 gccgcgcgat caggtcgccg cattcggaga cgatctggtc ggcatcctgc atgatcttga
     4741 gctgcttgtg gcgatcttcc cggtgcaacc ccatgctctc gccgaccact tcgaggatgt
     4801 tgatgatccg gaacggccag tcgcgctcat gcgcgcatag ttcgcgatgt tcggagtgat
     4861 agaccgtaat cagcgcatcg acgcccgcat cgcgcgccgc ttgaagctcg cccagttgca
     4921 gctcgcgctt gaacgccgga aggacgccga gactggcgct ctgcaagcca accgccggct
     4981 gtttcaagtc gacgagctcg acgcccggga ttgcctgcag gatttcagcg gcggcttcca
     5041 ttgcgccggc tacgccggga tgttggtgca tcgctactcg catttcgacc cgtcgccgca
     5101 ggtgcggtat caactgagcc agccggccgt cgagaaaccg catgaacggc gtcatctcga
     5161 acggccgcga gccgcgctgt cgctcgatcg tcggcagcgt cagttcggtg aactgaacgt
     5221 agcaactcgg gcaccaggag atcacctgcc cggatttgga gtgcgacagt ttctcgatcg
     5281 agttcgagcc catgcgcccg gacatctcga tgtcgcccgg gcgcagctgc acgacgccgc
     5341 aacaatggct tggtccgccc atcacctggt aggtgacgcc gagcacatcc atgatatcga
     5401 gggcaagcag cgcgatatgc ggcgtcttga gcacattgca gccggtgtaa aacacgaagt
     5461 ccggcgcctc ggcgggcgtc gataccgacg ccgatttctg gccgagccgc tccagcacct
     5521 cgccatcgag ttgcaagcgt gacagcacgg tcacatcgcg gctgagatcg cggtaccgct
     5581 ccacgccgag gcggcgccgg tcgggcagct cgctcccggc cttggcgatg gcgaggcgcg
     5641 ccatcgccag caggaagcgc ggattgaccc cgtagtcgca ggcctcgatg cactcgccgc
     5701 tgagcatgca agatgctgcc catctgcgcg acgcctcggg tccgtcgccg ctgcgtacga
     5761 tgtcgagcac gccgccgatg atgtcttcgg agctcgcttc cgcgatgccg gcaggtttca
     5821 cgctagggca gacctcgaca catttgccgc accgcgtgca ggcatcgagc atgtccgcga
     5881 cgcgctcgtc gagggccgcc tcgaaagaga tatcgctatt cggctgcata tccggccctt
     5941 ccgtgacgtt gaggaactag cagcgcaccc gtcgattttg atctcacgag cctaacccaa
     6001 accagttgag acgccaatgc cggggcggcg cgttgtcgca cggcaattcg atagggttca
     6061 tccgatcaac atgcgatggc ctgaggggat tcaggcagtg gcgttttgtg cgttagcgga
     6121 ctaaaaggcc cgcagcagcc cgtgattgcg ggaccgggag gaggttacgc gatggaacgc
     6181 atgcttcttc ggatcgcggt cgcggttgca ggccttgcgg gtgtgggcct gggccttacc
     6241 ggcgtcgtgt ttggcaccct ggatgccgat ttgtccggcg atgtggtgct ggacagctac
     6301 gttcgctttc tcaaggggat gctgctggcg atgggcctga tctactggtc ctgcatcccg
     6361 caaatcgatc ggtacggcga tcggatttcg ctggtgacgt tcattctcgt ggctggcacg
     6421 cttgcgcgcc tgatgtcggt gattggccac ggcgtgccga ccgtgggcat catgattaac
     6481 gtgatcgcgg gattggtctt cgtgccgctg ttgtggcttt ggcagcggcg cgtcgcccga
     6541 gtggcgcaac gtggcgccgt gacctgatga gacgtatcgc ggtctggatc ttctacgcgg
     6601 tcggcgcgct cgcgattctg tatctcgcgc tttacgccta tgcggcattc accggacatg
     6661 accttcagcc gggcgatccg atccacattt ttcggaagcc ggatgcgccc agttactcct
     6721 agactagaat gccgacgtca ttgcgagcca atgggttgcg cgaatgcgcg cccgatgacg
     6781 ggatccgcga agcaatccat cggcgtggaa acgcagactg gattgctcac atggggaatg
     6841 tgtgtgtcaa ggatgagcag tcaccttttt gttcgactgc ggccacctct tcgtcccgac
     6901 cgcgggctgt gcaggatgac gcgcgcagat caagtcaagg ccggcccttt agggccgc
//
LOCUS       DASWGM010000859         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2328812,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000859 DASWGM010000000
VERSION     DASWGM010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328812"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..513
                     /locus_tag="VGA15_23430"
     CDS             205..513
                     /locus_tag="VGA15_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX9590681.1"
                     /translation="MAKGCTHIAGIRSVTPSALGCEECLKIGSEWLHLRICRTCGHVG
                     CCDDSPHKHATSHFHATQHPVIEGYDPPEGWGWCYVDEVLFDLSDRMTPHNGPIPRYY
                     "
     gene            544..1380
                     /locus_tag="VGA15_23435"
     CDS             544..1380
                     /locus_tag="VGA15_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9590682.1"
                     /translation="MQAPQSKFAEVNGVRLHYLIAGTGDPVVLLHGYAETSHMWLPLI
                     AKLADKHTVIAPDLRGFGESAAPADGYTKAAMARDIHALVKNLNYDRIRLVGHDIGLM
                     VAYAYAAQYPGEVDRLVLMEAFLPGVGDWNSVFLLRDLWHFHFYGKTPLALVSGRERI
                     YLEHFWNDFAADPRKSVPEKDREFYAKAYAQPGHMAAGMEVFRAFPKDAEDFAGFAKT
                     RLTMPMLVLSGEKAGGPFLIEQGKMVASNVEGVLVKGSGHWLMEEAPDQVIPKLVEFL
                     NR"
     gene            1559..2071
                     /locus_tag="VGA15_23440"
     CDS             1559..2071
                     /locus_tag="VGA15_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590683.1"
                     /translation="MILGMSLATFTTVHVIISLIAIVSGILVMFGILGSSRMPGLTAI
                     FLLFTILTSATGFLIPPLMSEKLLPSHMIGILSLVLLAIACIALYGMRLAGAWRWIYA
                     VTAMISLYLNIFVLIIQGFLKVPALTALAPGNPPSGPVFAVVQGIVLLFFVLVIIGAI
                     RRFRPMPTYA"
     gene            2202..>2861
                     /locus_tag="VGA15_23445"
     CDS             2202..>2861
                     /locus_tag="VGA15_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9590684.1"
                     /translation="MATIKTKDGTEIYYKDWGSGQPVVFSHGWPLSADDRDTQMLFFL
                     NHGYRVIAHDRRGHGRSSQTGDGHDMDHYADDLAALTAYLDLKDAVHVGHSTGGGEVV
                     HYLGRHGESRVAKAAIISAVPPLMVKTPANPGGLPKEVFDGFQAQLAANRSQFYRDLP
                     AGPFYGYNRPGAKASEAIIQNWWRQGMMGGAKAHYDGIVAFSQTDFTEDLKKITVPVL
                     VM"
BASE COUNT          541 a          851 c          882 g          587 t
ORIGIN      
        1 tcggcgacgt gcgctcggga tcggtcaagc gggtcggcgg cgcgatcggc gagggcgcgc
       61 aggtggtggc ggcgctgcac ggttttcttg gcgatgccgc gaaaccggcg ctttagggca
      121 tgcgatgccc ggccgacacg acaaggattt tgagaccgtc attgcgaccc gttggctcgc
      181 aatgacgaaa ggacaggaaa ctgaatggca aaaggctgca cccacatcgc cggcatccgc
      241 agcgtcacgc cgagcgcgct cggctgcgag gaatgcctga agatcggcag cgagtggctg
      301 catctgcgga tctgccgcac ctgcggtcac gtcggctgct gtgacgactc tccgcacaaa
      361 cacgcgacca gtcattttca cgccacgcag catccggtca tcgagggcta cgatccgccg
      421 gaggggtggg gctggtgcta tgtcgatgaa gtcctgttcg acctgtcgga ccggatgacg
      481 ccgcataacg gtccgatccc gcgctattac tgagcaagcc tctcgataaa aaggaagaat
      541 cggatgcagg cgccgcaaag caagttcgcc gaggtcaacg gcgtcaggct gcattatctg
      601 atcgccggca cgggcgatcc ggtggtgctg ctgcatggct atgccgaaac cagccacatg
      661 tggctgccgc tgatcgcaaa attggccgac aaacataccg tgatcgcgcc tgacttgcgc
      721 ggcttcggtg aatccgcagc gcctgcggat ggttacacca aggcggcgat ggcgcgggac
      781 atccacgcgc tggtaaaaaa tctcaactac gaccgcatcc gtctggtcgg ccacgatatc
      841 gggctgatgg tggcctacgc ctatgcggcg caataccccg gcgaggtcga tcggctggtg
      901 ctgatggaag cgttcctgcc cggtgtcggc gactggaaca gcgtcttcct gctgcgcgat
      961 ctctggcatt tccatttcta cggcaagacg ccgttggcgc tggtctcggg gcgcgaacgg
     1021 atttaccttg agcacttctg gaacgatttt gccgccgatc caagaaaatc ggtgccggaa
     1081 aaagatcgcg aattttatgc caaggcctat gcccagcccg gccacatggc cgccgggatg
     1141 gaagttttcc gcgccttccc aaaagacgcc gaggatttcg caggatttgc aaaaaccagg
     1201 ctgacgatgc cgatgctggt gctttcaggg gaaaaagccg gcggtccgtt cctgatcgag
     1261 cagggcaaga tggtggcgag caacgtcgag ggcgtgctcg tcaagggatc gggacactgg
     1321 ctgatggaag aagcgccgga ccaggtgatc ccgaagctgg tcgaattcct caatcgttag
     1381 aataccgtca gcatcgttgg cgctgtttgc gacagccatg caaaaaactt gggcatggcc
     1441 tccgcttttt ttgatcgaag ccgcatgcct gaattgccgc cggcaccacc ggagcatgtg
     1501 gcaatttcct ttccccggtc gtaccatttg ccgcggtttg taaaaggggg gacgcgtcat
     1561 gatcctaggt atgagtttag ctacattcac cacggtgcat gtgatcatca gtctgatcgc
     1621 gatcgtttcg ggcattctgg tcatgttcgg gattctcggc tccagccgga tgccgggcct
     1681 gaccgcgatc ttcctgctgt tcacgatttt aaccagcgcc accggctttc tgattccgcc
     1741 tctgatgtct gagaagttgc tgccctcgca catgatcggc attctctcgc tggtcttgct
     1801 ggcgatcgcc tgtatcgcgc tttacggcat gcggcttgcc ggcgcctggc gctggatcta
     1861 tgccgtgacc gcgatgatct cgctctatct caacatattc gtgctcatca ttcagggatt
     1921 cctgaaagtg ccggccctga cggcgctggc gccgggtaat cctccgtctg gaccggtgtt
     1981 cgctgtggtg caaggcatcg tgctgttgtt tttcgtcctt gtgatcatcg gtgccatcag
     2041 gcgatttcgg ccgatgccga catatgcctg agcggccttt gggcgtcatt ccgggcgaca
     2101 cgaagtgtcg aacccggaat ctcacgccat catctctgga ttttcgggtt cagccctgcg
     2161 ggctgccccg gaattgacgg ccttcacaaa ggagtcctgc catggcaacg atcaagacca
     2221 aagacggcac ggaaatctac tacaaggact ggggcagtgg tcagccggtg gtgttctcgc
     2281 acggctggcc gctgtcggcc gatgatcggg acacgcagat gctgttcttc ctcaatcacg
     2341 gctatcgggt catcgcccat gaccgccgcg gccacggccg gtccagccag accggcgacg
     2401 gccatgacat ggatcactat gcggacgacc ttgcggcctt gacggcgtat ctcgatctga
     2461 aggacgccgt tcacgtcggc cattccaccg gcggcggcga ggtggtgcac tatctgggac
     2521 ggcacggcga gagccgggtg gcgaaggcgg ccatcatcag cgcggtgcca cccttgatgg
     2581 tgaagacacc ggccaatccc ggcggcctcc ccaaagaggt ctttgacgga tttcaggcgc
     2641 agctcgccgc caatcgctcg caattttatc gcgaccttcc ggcgggtccc ttctatggct
     2701 acaaccggcc gggcgccaaa gcctcggagg ccatcatcca gaattggtgg cgccagggca
     2761 tgatgggcgg cgccaaggcg cattacgacg gcatcgtcgc cttctcacag accgatttca
     2821 ccgaagacct caagaagatc accgtgcccg tcctggtgat g
//
LOCUS       DASWGM010000860         4881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_642544,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000860 DASWGM010000000
VERSION     DASWGM010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642544"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VGA15_23450"
     CDS             complement(<1..198)
                     /locus_tag="VGA15_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590685.1"
                     /translation="MRPFIVRRRRHATSDHHAKRHDKDQERYGYRFGGKDDGGGRQTL
                     CLMRPANQSGSHIHHTASCFAR"
     gene            complement(583..1468)
                     /locus_tag="VGA15_23455"
                     /pseudo
     CDS             complement(583..1468)
                     /locus_tag="VGA15_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
     gene            complement(1502..2386)
                     /locus_tag="VGA15_23460"
     CDS             complement(1502..2386)
                     /locus_tag="VGA15_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590686.1"
                     /translation="MSDMALANQQRPLKMTRPRRFFRWLATDLRAALAVFVLLMLVVV
                     AIGAPWIAPYQPTAQDVNNMLAAPSASHLLGTDDLGRDIFSRLIYGAPATVYASLLAV
                     SVAVAIGLPVGLMAGFFGGWIDDIMSRAIDTFLSFPAIVLAIAVTGALGIGLTNGMIA
                     VGIVMFPALARIMRARTLIVRQELYVDASRCFGAPAWHILWKHVLPNSLQPVIVQVTL
                     LLAAALLAEASLSFLGLGIQPPNPSWGAMLARAYQYMEIAPEQMYAPGLAILVVSLAF
                     NALGESLRIVLDPTMRDR"
     gene            complement(2383..3339)
                     /locus_tag="VGA15_23465"
     CDS             complement(2383..3339)
                     /locus_tag="VGA15_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590687.1"
                     /translation="MARRSPAGVIGSRLLQALPVILLATFMVFSLLKLVPGDIAVTLA
                     GDNASDTRIAEIRKIYGLDRSFLVQYGVWLGHVVQGDLSQSLLSGEKVATSISRAFPN
                     TLLIVAIALVLALAAGIPMGVVGAIKPNGWVDKLVSMVASLGVAIPGFWLAMILVAQI
                     SLKLNWLPATGAKSFAVSPLDAIRHALLPGIAIAAYGMAEVARQLRGSLLEVLSSQYV
                     RTLHAKGLSMTRILWQHGLKNVSVNLFTIISLLVNRMLAATVVIEAVFAIPGLGSLIV
                     RGAIQRDFPIVQGVVFTMVIVVIVVNLIADLLCAAVDPRIEQ"
     gene            complement(3343..>4881)
                     /locus_tag="VGA15_23470"
     CDS             complement(3343..>4881)
                     /locus_tag="VGA15_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9590688.1"
                     /translation="ATRRDAMALISGAVAGVALAGKARAQGAPKRGGILRISAPTNPS
                     SLDPATGGAGSDHAFLFTMYDTLTEWDFETLKPKPGLAESWSFTDPTTLVLNIRPGVT
                     FHDGTPLDAEAVKFNLERNKSDPKSNIKADLATMASVELTGPMQVTLKLNTPDAALPG
                     ILSDRAGMMVSPTVLKASGAGNVARTPVGAGAYAFVSWADGERIVIKRNEKYWKESRP
                     YPDGIEFAIIPELTTGVRSVTAGQNDLIYQLPPRQKAIVERSNNLKVVNGPTLYVFQI
                     FLNWAKPPFDDVRVRKAFNLAIDRESFVKAALAGLAEPAYMNLPKSHWAYDRSVADLN
                     PYDSDRARKLLVEAGFKEGTVIELGGYPDQDSVQRQEILIEQLRKAGIGIRFVNAPVA
                     EASAAFFGPEKRGSGLLAAWTGRPDPSLTYSLMFTKDAYYNGGRAPVPPELEAAIKES
                     RASEDIEIRRKAFAVVQKLVMENAFVVPLAFQYELLAMNKRVQGYRPNLLGKPKYDDV
                     WLEN"
BASE COUNT          993 a         1511 c         1467 g          910 t
ORIGIN      
        1 tcttgcaaag cacgaggcgg tatgatggat atgcgacccg gattggttcg cagggcgcat
       61 caagcagagc gtctgacgcc ctcccccatc atccttcccg ccgaagcgat agccgtaacg
      121 ctcctgatcc ttgtcgtggc gcttggcgtg atgatccgaa gtggcatgac gccggcggcg
      181 cacaatgaat gggcgcatgc cggttgcgag ttggacgctc ggtaacagtc gggaggtcgc
      241 gtttgtgtga attgtccgtt ccggctagcg tggaatggtc cagcggtggc cccgaaaaat
      301 cggcaagcca tgacgcggcg tatgtgcaat gggtgagcca tgaaaagtca caccaaactc
      361 gccgccgcct tgctgcttct cgtcggcgtg gttgaattct caaatatcgc cccggccttg
      421 gcgcggggtg gtgcgccgag catcatgaat tcccccggtt atcaacggcg cctccaggaa
      481 tccaggcagc cagactctcg accctatgcg cagacgctgg cgatcgaccc ccgaaagaaa
      541 cggcatcatc gccaccaccg ccattaagag cctctcgata tcctaccagg catcgatgca
      601 gggccgcttg cggtggctgc gtggttcggg ctttggcacg gattgaaggc cagccatgat
      661 ccaacggcgg gtctcggccg gatcaatcac ttcgtcgatt tccatgacag atgcgataga
      721 ggtcgccttg ccgttggcat agagctccgc gaccttggcc ttgtagtatt tctcccgctc
      781 gacgtgatcg gcgatcgctt ccatttcctt gcggaagcca agccgcacat agccctccag
      841 gcctatgccg ccgaactcgc cggtcggcca tgccaccgtg aagaaggaag catgaaaacc
      901 gccgccgatc atggattgcg cctcgaggcc gtaacccttg cgcagcacaa cgccgaacag
      961 cggcacgggg aggcttgccc ccgtcacgaa catgcgggcg acatggcgcc ggaccaacct
     1021 cggctggatg gtagacgcca agaccaccac cctcgatcat cgctggcccg cccatgccaa
     1081 tcgacgcatt cttcgtcgca atgatgacgt cgcagcaacc gagcattgca gcgttgccgg
     1141 caaagcaata gccggacacc acgcccacca ccggcaccag gccggacagc tttgcgaact
     1201 gcacgaatga cggaccatcg agaccggtca tgccaagccg gtcggtgtcg ccggggcgac
     1261 cgccgccgcc ttcggcataa aacaccaatg gcattcgcca ttgctcggca aggccgagca
     1321 tgcggtcgat cttcttgtgg ttcatatgac cttgtgtgcc ggccaacacg gtatagtcgt
     1381 aagaaatcac catgcagcgc gcgtctttcg caccgaattt gtccgcgttg accgtcgcaa
     1441 cgcctgtgat caggccgtcg gccggtgtgt acgaaagggc atgaaatgtt tccgttaaga
     1501 ctcagcgatc cctcatggtc ggatcgagca cgatgcgcaa ggactctccg agcgcattga
     1561 acgccagcga aaccaccaga atggcgaggc caggcgcata catctgctcc ggcgcgatct
     1621 ccatgtattg ataggcgcgg gccagcattg cgccccagct tgggttgggc ggctggatgc
     1681 cgagaccgag aaagctgaga cttgcttccg cgagtagcgc tgccgcgagc agcagcgtca
     1741 cctggacgat cactggctgc aatgaattcg gcagcacatg cttccaaagg atgtgccagg
     1801 ctggagcacc gaagcagcgc gacgcatcga catagagttc ctggcgcacg atcagcgtgc
     1861 gcgcgcgcat gatccgtgcc agcgccggaa acataacaat gccaacggcg atcatgccgt
     1921 tggtcaggcc gatgcccagg gccccggtca ccgcgattgc caggacgatg gccggaaatg
     1981 acagaaaagt gtcgatcgct cggctcatga tgtcatcgat ccagccgcca aaaaatccgg
     2041 ccatcaatcc gacgggtagc ccgatggcaa ccgccacgct caccgcaagc aggctcgcat
     2101 acaccgtagc cggtgcgcca tagatcaacc ggctgaagat gtcgcggccg agatcgtcgg
     2161 tgccgagcag atgacttgca ctcggcgccg ccagcatgtt gttgacatcc tgcgcggtgg
     2221 gttgatacgg cgcaatccac ggcgcgccga tcgcgacgac gacaagcatg agcaggacga
     2281 agaccgccag cgccgcgcgc aagtccgtgg ccagccatcg gaagaaacga cgcgggcggg
     2341 tcattttcaa agggcgttgc tggtttgcca gcgccatgtc gctcattgct cgatcctcgg
     2401 atcgaccgcc gcgcacaaca gatcggcgat caggttgacc acaataacga cgatcaccat
     2461 ggtgaacacc acaccctgca cgatcggaaa atcgcgctga atggctccgc gaacgatcaa
     2521 gcttcccaag ccgggaatgg cgaacacagc ctcaatgacc acggtcgcgg ccagcatgcg
     2581 gttcaccagc aggctgataa tggtgaacag attgacgctg acgttcttga ggccgtgctg
     2641 ccagaggatt cttgtcatcg acagtccctt ggcgtgcagc gtccgcacat attgcgacga
     2701 cagcacctcc agcagcgaac ctcgtagctg ccgcgccacc tcggccatgc cgtaggcggc
     2761 gatggcgata ccgggcagca acgcgtgccg gatcgcgtcc aagggcgaca cggcaaagga
     2821 ttttgcgccg gtagccggca gccagttcag tttcagcgag atttgcgcca ccagaatcat
     2881 ggcgagccag aatcccggta tcgcaacgcc aagcgaggcg accatgctga caagtttgtc
     2941 gacccagcca ttgggtttga tcgccccaac gacacccatc gggattcccg cagcgagcgc
     3001 caacaccagc gcaatggcta cgatcaagag cgtatttgga aaagcccggg agatcgatgt
     3061 tgcaaccttc tcgcccgaga gcagcgattg cgagaggtcg ccctgcacca cgtgtccaag
     3121 ccatacgccg tattgcacca ggaacgaccg atcgagaccg tatatcttcc tgatctcggc
     3181 gatgcgcgtg tccgaggcgt tgtcgcccgc cagcgtcacc gcaatgtcgc cgggcaccag
     3241 cttgagcagt gaaaacacca tgaaagtcgc gagcagaatc accggcagcg cctgcagcag
     3301 ccggctgcct atgaccccgg ccggacttcg ccgcgccatg ccctagttct ccagccagac
     3361 gtcgtcgtat ttcggcttgc cgagcaggtt cggtctgtag ccttgcacgc gcttgttcat
     3421 cgcgagcagc tcatactgga atgcgagcgg cacgacgaag gcattctcca tcacgagctt
     3481 ttgcacgaca gcgaaagcct tgcggcgaat ctcgatgtct tccgaggcac gcgattcctt
     3541 gatcgcggct tccagttccg gcgggaccgg agccctgccg ccgttgtaat aggcatcctt
     3601 ggtgaacatc agggagtacg tcaggcttgg atcaggtcgc ccggtccagg cggcaagcaa
     3661 cccggagccg cgtttttcgg ggccgaaaaa cgccgccgag gcttccgcaa ccggtgcgtt
     3721 gacaaagcgt atgccgatgc cggccttgcg gagttgttcg atcaggattt cctggcgctg
     3781 aaccgaatct tgatccgggt agccgccgag ttcgatcacc gttccttcct tgaagcccgc
     3841 ttcgacgagc agcttgcgcg ccctgtcgga atcgtaaggg ttgaggtcgg cgacagatct
     3901 gtcgtaggcc caatgcgatt tcggcaagtt catgtaagcc ggctcggcaa gtcccgcgag
     3961 tgcggctttg acgaaggatt cccggtcgat cgcaaggttg aacgccttgc gaaccctgac
     4021 gtcgtcgaac ggcggttttg cccagttcag gaaaatctgg aagacgtaga gcgtcggccc
     4081 attgaccacc ttgaggttgt tcgagcgttc cacgattgct ttctggcgcg gaggcagttg
     4141 ataaatcaaa tcgttctgtc ccgcggtgac cgagcgcacg ccggttgtca gttcggggat
     4201 gatcgcaaac tcgatgccat cgggataagg acggctttcc ttccagtatt tttcgttgcg
     4261 cttgatgacg atacgttcgc cgtccgccca gctgacaaag gcataggccc cggcgcccac
     4321 cggcgtacgg gcgacgttgc cggcgccgct tgccttgaga accgttggcg agaccatcat
     4381 gccggcgcga tccgaaagaa taccgggcaa cgccgcgtca ggcgtgttca gcttcagggt
     4441 gacctgcatc ggacctgtga gctctaccga tgccatggtc gcaagatcgg ccttgatgtt
     4501 ggatttcgga tcgctcttgt tgcgctccag attgaatttg accgcttcgg catcgagtgg
     4561 cgtgccatcg tgaaaggtca cacccggccg gatgttgagc acgagtgtcg ttgggtcggt
     4621 aaaactccag ctttcggcga gacccggctt gggtttcaag gtctcaaagt cccactcggt
     4681 caaggtgtcg tacatggtga acagaaaggc atggtccgag ccggccccgc cggtcgcagg
     4741 atcaaggctc gacgggttgg ttggagccga gatgcgcaag atgccgccgc gtttcggcgc
     4801 tccttgcgcg cgcgccttgc cggcgagcgc cacgcccgcg actgcgcccg atatcagcgc
     4861 catcgcgtcg cgtcgtgtcg c
//
LOCUS       DASWGM010000861         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2712356,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000861 DASWGM010000000
VERSION     DASWGM010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2712356"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(538..783)
                     /locus_tag="VGA15_23475"
     CDS             complement(538..783)
                     /locus_tag="VGA15_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590689.1"
                     /translation="MWTRWTCVAAICLGLAPPAKAAERSGLVHRASCTVVRFYVAKFS
                     AFAAETWARNHGATEAEIDAARHCLKDAPAQTAQAGQ"
     gene            complement(1004..1753)
                     /gene="recO"
                     /locus_tag="VGA15_23480"
     CDS             complement(1004..1753)
                     /gene="recO"
                     /locus_tag="VGA15_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX9590690.1"
                     /translation="MEWTDEGIVLGVRRHGESSAIVELLTRGHGRHLGLVRGGAGSRM
                     RPLLQPGNSVRAVWRARLDEHLGYYQIEGTRLRAAAVLASSHAVYGVTHMASLARLLP
                     ERDPHEDIYEMLERTLDDFDDIGEAAVHLVKFELAMLAELGFGLDLETCAATGGTSDL
                     IYVSPKSGGAVSRQAGDPWRERLLRLPPFLRSSGGGPNGWSDQDLQDGFALTGMFLLR
                     HVLEPRGQGHSDARDGFINAVTKHRARMAVP"
     gene            complement(1867..2247)
                     /locus_tag="VGA15_23485"
     CDS             complement(1867..2247)
                     /locus_tag="VGA15_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590691.1"
                     /translation="MNVPKNVIWFEVLLYLSLTLDALSVAFQDRTPSAEMTEQMITVA
                     TVMAACLILLLVYFVWLAAQRRKNWPRWALAAAGVLSVIQLMQIIADGGLQLDSGIEI
                     VSCILTAAGLYFSFTGDAQGWFNG"
     gene            complement(2260..>2887)
                     /gene="era"
                     /locus_tag="VGA15_23490"
     CDS             complement(2260..>2887)
                     /gene="era"
                     /locus_tag="VGA15_23490"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX9590692.1"
                     /translation="LLDAREGIDEEADAILTKVATVRHERILVLNKIDLVPREKLLAL
                     TKAANDRLSFEKTFMVSALSGDGVDDLRHTLAEMVPPGPFHYPEDQMSDAPMRHLAAE
                     ITREKIFRQLHQELPYQSTVETDSWTERKDKSIRIEQTIFVERESQRKIVLGKGGATI
                     KSIGADSRKEIAEIVGVPVHLFLFVKVRENWGDDPNRYREMGLEFPKE"
BASE COUNT          554 a          925 c          860 g          548 t
ORIGIN      
        1 atattggata tacagaccct tctgcatggc aaggaacggc gttgcgcatt cgatgcacct
       61 cggcttgcgc agggaagcgt cggtccgctg cacggcaacg tattcggcgc gacattccaa
      121 gtttgggcat ctgtagttgt acggggtcga atcgctcatc gccgcagtct agcagagacg
      181 cgctatctca gggtgttcca ctggcggaac acccctttca cgaaatgttc ttggggcgtt
      241 taagcgcgcc cctcaaacgt gcccatcaac gccgtggtgc gatccgtctc cggatgggcc
      301 gccgctgtac tgattgtcgg gattgtccgt gtctggcgtc accttcaaac cttggcgaaa
      361 tgcaaagcca atgaacccta cgaggacgac caatgcgaga agtggcccac ccaattgggc
      421 atggtcggct ccattcaggc cgcaatctgc ggctattcgg ccattccacg ctcgccgggt
      481 tggacgccag tgaacgccta tggaacgttt ttgggaaact gggtgcggaa actttgatta
      541 ttgaccggcc tgcgcggtct gggcgggcgc gtctttgagg caatgccggg cggcgtcgat
      601 ttcggcttcg gtggccccgt gattgcgcgc ccatgtctcg gcggcgaacg ccgagaactt
      661 ggcaacgtag aaccgaacga ccgtgcacga cgcgcgatga accagtccgg agcgctcggc
      721 agccttcgct ggcggagcca gccccaggca aatcgcagcg acgcacgtcc agcgtgtcca
      781 cattatccaa aaccccttct tgccccagtt caagttgctc gacgcagcta agttccattg
      841 atggaacacg catttagcgc cgacccaaga gaaaattagc cgactcgccg attgaaaaac
      901 aaggtcgcac tgcaccgcgc tgaatttacc cccgctattg ttgcatcttt gttgtggcgc
      961 ggagcccgtc cttcggcacc agcgccgcaa atcgggttcg gctctaagga accgccattc
     1021 tcgcccggtg tttcgtcacc gcgttgataa aaccatcgcg ggcgtcggag tggccctgcc
     1081 cgcgcggctc cagcacgtgg cgcaatagga acatgcccgt cagcgcaaaa ccgtcctgaa
     1141 gatcctgatc cgaccaaccg ttcgggccgc cgccggacga gcgcaagaac ggcggcaacc
     1201 gcagcagccg ctcgcgccag ggatcgccag cctgccgcga caccgcgccg ccggatttcg
     1261 gcgacacata aatcagatca gacgtcccgc cggtggcggc acaggtttcc agatcgagcc
     1321 caaaaccgag ttcggcgagc atcgccagtt cgaacttgac gagatgcacc gcggcttcgc
     1381 cgatgtcgtc gaagtcgtcg agggttcgtt ccagcatctc gtagatgtct tcgtgcggat
     1441 cgcgctccgg caacagccgc gccagcgagg ccatatgcgt cacgccatag accgcgtgcg
     1501 acgacgccag cacggcagcc gcgcgcagcc tcgtgccctc gatctggtaa tagccgagat
     1561 gctcgtcgag ccgcgcccgc cacaccgcgc gcacgctgtt gccgggctgc agcaagggcc
     1621 gcatccgcga acccgccccg ccgcgcacca ggccgaggtg gcggccgtgg ccgcgcgtca
     1681 agagttcgac gatggctgag gattcgccat gccgccgcac ccccagcacg atgccttcgt
     1741 cggtccattc catggagtgg agtgtacagg attccgttgc tggacgcagc ttctgtgact
     1801 gcgtgccccg ggcgccgcgc agcgcgctct cggcgatgcg aagcgtccgg gacacgagac
     1861 tgtgagctat ccgttgaacc agccctgggc gtcgccggta aaggaaaaat acagcccggc
     1921 agccgtcagg atgcatgaaa cgatctcgat gccgctatcg agctgcaggc caccgtcggc
     1981 gatgatctgc atcagctgga taaccgaaag cacccctgcg gcagccagcg cccagcgcgg
     2041 ccagtttttc cgccgttgcg cggcgagcca gacgaagtag accaagagca ggatcaggca
     2101 ggccgccatc acggtcgcga ccgtgatcat ctgttccgtc atctccgcgc tcggcgttcg
     2161 atcctggaac gcgaccgaca gcgcgtcgag cgtcagcgac agatacagca gcacttcaaa
     2221 ccaaatgaca tttttcggta cgttcatcgc gggcgccggc tattccttcg ggaattcaag
     2281 tcccatttca cggtagcggt tgggatcgtc gccccagttt tcgcgcacct tcacgaacaa
     2341 aaacaggtgc acggggacgc ccacgatctc ggcgatttcc ttgcgtgagt ccgcaccgat
     2401 cgacttgatg gtggcgccac ccttgccgag cacgatcttg cgctggcttt cgcgctcgac
     2461 aaagatcgtt tgctcgatgc ggatggactt gtccttgcgc tcggtccagc tatcggtctc
     2521 aacggtggat tgatatggca gttcctgatg caactggcgg aagatttttt cccgggtgat
     2581 ttcagccgcc agatgccgca tcggtgcgtc cgacatctga tcctcgggat agtgaaacgg
     2641 gcccggcggc accatctccg ccagcgtatg cctgaggtcg tcgacgccat cgccagacag
     2701 cgccgaaacc atgaaggtct tttcgaaact caggcggtca ttggccgcct tggtcagcgc
     2761 cagcagtttt tcgcgcggaa ccagatcgat cttgttgagc accagaattc gctcgtgacg
     2821 aacggtcgcc accttggtga ggatggcgtc ggcttcctcg tcaatgccct cgcgcgcatc
     2881 gagcagc
//
LOCUS       DASWGM010000862         5965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_376879,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000862 DASWGM010000000
VERSION     DASWGM010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376879"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /gene="lnt"
                     /locus_tag="VGA15_23495"
     CDS             <1..748
                     /gene="lnt"
                     /locus_tag="VGA15_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX9590693.1"
                     /translation="ILIWPESAFPFFLTREADAMAQISDLLPKGTILITGSVRAPDLP
                     PGTKITRAYNSIYAIDHDGSVLSIYDKLHLVPFGEYLPYQDWMEKLGFVQLTKVQGGF
                     IPGARRRPMEFPNAPRALPLICYEAIFPGSTAVGDERPGWIVNLTNDGWFGISTGPYQ
                     HLQQARLRGIEQGLPVVRAANTGISAVIDPLGRIVGRLGLGIEGVLDSSLPSAIAPTI
                     YARTGDIPAAVVVATALIFIIRRRVAKRTF"
     gene            998..1414
                     /locus_tag="VGA15_23500"
     CDS             998..1414
                     /locus_tag="VGA15_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9590694.1"
                     /translation="MSTKAPNPVDKYVGSRVRMRRIMLGMSQEKLGEALGLTFQQVQK
                     YEKGTNRVGASRLQQISEILQVPVSFLFEGGPSGATNAGGFSQGTSPSYVSDFLATSE
                     GLALTRAFTRISDAKLRRSIVEMVEQIAMREGPDER"
     gene            1694..2830
                     /locus_tag="VGA15_23505"
     CDS             1694..2830
                     /locus_tag="VGA15_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX9590695.1"
                     /translation="MTTANSSNPFDAKTILDGIRRWVEIETPTDAPDQVNRLATLVAD
                     GYRDLPVTIERVAGHSGCGDHILARSSWGKDTPGILVLSHLDTVHPMGFIERLPFRVE
                     GDSAFGPGIYDMKGGAYLAYHAFRQLCGDDERSPLGVTQLYVSDEEIGSPTSRALIEA
                     EGRKAKYVLVTEPARDGGKIVTGRKGVARFEIFVHGVPAHAGSRPEDGRSAIRELANV
                     IQTLEGLTDIKRGVTVNVGVVRGGTRPNVVAEEAYAEVDMRVRTIADADEFVPKILNL
                     KSRTEGVSVKVVGELNRPPYEKGNAGAALFEHAKTLAAEIGFDLVDTFTGGGSDGNFT
                     APHTATLDGLGVDGQGAHTHYEQMYISSIEPRVRLLHRLYQTLR"
     gene            2827..3582
                     /locus_tag="VGA15_23510"
     CDS             2827..3582
                     /locus_tag="VGA15_23510"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HEX9590696.1"
                     /translation="MSVAARDPTDRDTNGNASPDDGTAAPTHGSFFGRRKGHKLRLHQ
                     ADLIEHLLPHLSLDIGKPGPPDLADLFAAPVDDLRLEIGFGGGEHLIAEARAFPRIGF
                     IGCEPYVNGMAKILTQIEAHNIGNIRLFAGDAAELLAWAPPCSLARIDLIHPDPWPKR
                     RHWKRRFVQDLTVAAMARVLRPDGEFRFVSDIADYCAWTLAHLSRSPDFVWTAERASD
                     WRLPWADYTMTRYGRKAEREGRHAAYLRFRRIE"
     gene            complement(3632..4714)
                     /locus_tag="VGA15_23515"
     CDS             complement(3632..4714)
                     /locus_tag="VGA15_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEX9590697.1"
                     /translation="MTIARSLIPELDEIVRHGDPRRRAEAARRITELFLQGAATFRSD
                     HVDLFDGVLIDLVPHTELAARADLAERLSLLANAPRALVGHLAREDEIAIAGPLLRRS
                     PVIDERALIEIARIKGQGHLLAMSERPTLSCDLTDVIIRRGDRAAVRRAAGNAGALFS
                     QSGYSALIRRAGQDGVLTLAIGKRDDLSSQQLRDLLAGSIDIVRRRLFEVVKPGRQAD
                     IKMAMSEISGEAEPVESGRDFAPAQRTILALHHAGELNQSALLGFAKAYKYEESIAAL
                     SAMSAVKIAALDRLIMGDRHDPILIVGKAVGLEWATVRALILLRLGPNRVPSPADIES
                     ARVNFGRLMPSTAERVVSFWQTRQSA"
     gene            5066..5836
                     /gene="rimP"
                     /locus_tag="VGA15_23520"
     CDS             5066..5836
                     /gene="rimP"
                     /locus_tag="VGA15_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX9590698.1"
                     /translation="MTDPTAGSVDTDLLAEPRLVVEPGMAARVSAVAGPVLQGMGYRL
                     VRIKISGEFGCTVQIMAERPDGSMQIDDCEAISRALSPVLDVADPIDRAYRLEISSPG
                     IDRPLVRRSDFERYAGHLVKIEMAAAHQGRKRFRGTLGGVEGDAVHLHRDDIRAGEDA
                     DVVLVMEDIADARLVLTDELIAESMRRGKAAERELKQSLGLAPPPPPYARKSDPARSN
                     KPKLKPKPEKKSAPKNTKEHRLAAERRRRGEFDPTEGD"
     gene            5840..>5965
                     /locus_tag="VGA15_23525"
     CDS             5840..>5965
                     /locus_tag="VGA15_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX9590699.1"
                     /translation="MAAVSANKLELLQIADAVAREKSIDRGIVIAAMEDAIAKAAR"
BASE COUNT         1207 a         1867 c         1837 g         1054 t
ORIGIN      
        1 cattttgatc tggccggaat cggcgtttcc gtttttcctg acgcgcgaag ccgacgcgat
       61 ggcacaaata tccgacctgt tgccgaaggg caccatcctg atcaccggct cggtgcgcgc
      121 gcccgatctg ccgcccggca cgaaaatcac acgcgcctac aattcgatct acgccatcga
      181 ccatgatggc agtgtgctgt cgatctacga caagctgcac ctggtaccat tcggcgaata
      241 tctgccctac caggactgga tggaaaaact cggcttcgtc caactcacca aagttcaggg
      301 cggatttatt ccaggcgccc gtcgccgccc gatggagttt ccgaacgcgc cgcgcgcgct
      361 acctctgata tgctatgaag cgatatttcc cggaagcacc gccgtgggcg acgagcgtcc
      421 aggctggatc gtcaacctaa cgaatgatgg ctggttcgga atctcaaccg gcccgtatca
      481 gcatctgcag caggcgcgcc tgcgcgggat cgagcagggg ctgcccgtgg ttcgcgcagc
      541 aaacaccggg atttccgcgg tgatcgatcc gctgggccga attgtcgggc ggctcggtct
      601 cggcatcgaa ggcgtgctgg actccagcct gccgtcggct attgcgccga cgatttacgc
      661 acgcaccggc gatattccgg cagctgtcgt cgtcgccacc gccctgattt tcatcatccg
      721 gcgacgcgtc gcgaagcgaa ctttctgacg ctcacagctt gatgcttgaa ctagcttgag
      781 ggcagaaaaa cggcatgggt aaaaccacta attgacagat cggctgtgca attcgcattg
      841 tcccgcgccg tcgaggattg atccagacag ttaaatagct aaaattgtct gcattcacca
      901 actctccgag caggatttga cggtattggc gcccggggca tacaacgttt tcgttctgcc
      961 tcaccaccag cgcgcgtaat cccaaggagt gttggaaatg tcgactaaag cgcccaaccc
     1021 tgttgacaaa tatgtcggca gccgtgtgcg tatgcgccgt atcatgctcg gtatgagcca
     1081 ggaaaaacta ggcgaagcgc tgggcctcac attccagcag gttcagaaat acgaaaaagg
     1141 caccaaccgg gtcggggcaa gtcgcctgca gcaaatatct gaaatacttc aagtaccagt
     1201 gtcgtttttg ttcgagggcg ggcctagcgg cgccaccaac gccgggggtt tcagccaagg
     1261 aacgtcgccg tcctacgttt ccgattttct cgcgacctcc gagggtttgg cgctaacccg
     1321 cgcgttcacg cgcatctcgg acgccaagct tcggcgcagc attgtcgaga tggtcgagca
     1381 gatcgccatg cgcgaaggac ccgatgagcg ctagatgaga ttcgtgattc cgcgggtcga
     1441 aattggcgca tcgacgcgcg aggactgcga caaaattgcc gcatccggct acaatctgcg
     1501 gtagacgggc gagcccggaa cccaggacaa ccgccgggtg tgtcgcttcc gggcctgcgc
     1561 catcccaccc atgcgtcgcc gaccggacgt tgatatgctt aaccggcgaa cccgcgttcg
     1621 gtgaacgcgt ccacagatgt gcaactgcac atcgggaatg aagaatagtt aaaattcctc
     1681 ggaaggcggc agcatgacga cggccaattc ctccaaccct ttcgatgcca aaaccattct
     1741 cgacggcatc cgccgctggg ttgaaatcga gacgcctacg gatgcgccag accaggtcaa
     1801 cagactggcg acgctggtcg cggacggcta tcgcgatttg cccgtcacca tcgagcgcgt
     1861 cgctggacac tccggctgcg gcgatcatat cctggcgcgt tcgtcatggg gcaaggacac
     1921 gccggggatc ctggtgctga gccatctcga caccgttcat cccatgggat tcatcgaacg
     1981 gctgccgttc agggtagagg gcgacagcgc cttcggtccg gggatctacg acatgaaggg
     2041 cggcgcctat ctcgcctatc acgcgttccg ccagctttgc ggagacgacg agcgttcgcc
     2101 gcttggcgtc acccagctct acgtctcgga tgaggagatc ggcagcccga cgtcgcgcgc
     2161 gctgatcgag gccgagggac gcaaggcaaa atacgtgctg gtcactgagc ctgcccgcga
     2221 cggcggaaaa atcgtcaccg gccgcaaggg tgtcgcgcgc tttgaaatct tcgtacacgg
     2281 tgttcccgcc catgccggca gccggcccga ggatggccgc agcgcgatac gcgaactcgc
     2341 caacgtcatc cagacgctgg agggcctgac cgacatcaag cgtggcgtaa ccgtcaatgt
     2401 cggcgtagtc aggggcggca cgcggcccaa cgtcgtcgcg gaggaggcct atgccgaagt
     2461 cgacatgcgc gttcgcacca tcgcggatgc tgacgagttc gtgccaaaaa tcctcaacct
     2521 gaaatcccgg accgaaggcg tcagcgtcaa ggtcgtcggc gagttgaacc ggccgcccta
     2581 cgagaagggc aatgccggcg cagcattgtt cgaacacgcc aaaacgctcg ccgcggaaat
     2641 cggcttcgat ctggtggata ccttcaccgg tggcggctcc gacggcaatt tcaccgcacc
     2701 gcataccgcc acactcgacg gcctcggcgt cgatggccag ggcgcgcata cccattacga
     2761 gcagatgtat atttcgtcga tcgagccgag ggtacggctg ctgcatcggc tgtatcagac
     2821 gctgcgatga gtgtcgcagc ccgcgatccc accgatcgcg acacgaacgg caatgcatcg
     2881 cccgatgacg gcactgccgc acccacccac ggttcgtttt tcggacggcg caagggccac
     2941 aagctccgtt tgcaccaggc cgacctgatc gaacacttgc tgccgcacct ttcgctcgac
     3001 atcggcaagc ccggcccgcc cgacctcgct gacctgtttg cagcgccggt cgatgatctc
     3061 aggctcgaaa tcggattcgg cggcggcgaa catctgatcg ccgaagcccg cgcctttcca
     3121 aggatcggct tcatcggctg cgaaccctat gtcaacggca tggccaagat cctgacgcag
     3181 atcgaagccc ataacatcgg caacatccgg ctgttcgcgg gcgacgccgc ggaactgctg
     3241 gcatgggcgc cgccatgctc gctggcgagg atcgatctga tccatcccga tccgtggccg
     3301 aagcgaaggc actggaagcg gcgcttcgtg caggacttaa ccgtcgccgc gatggcgcgc
     3361 gtgttgaggc cggacggcga atttcgcttc gtcagcgata tcgcagacta ctgcgcgtgg
     3421 acgcttgcgc atctttcacg ctcgcccgat ttcgtctgga ccgcagaacg ggcctccgac
     3481 tggcgcctgc catgggccga ctacacgatg acgcgatacg gacgcaaagc cgaacgcgaa
     3541 ggccgccacg cggcctatct gcggttccgg cgcatcgaat gatgcgggag aaattcgcca
     3601 ccgtcattgc ggggcgcgcg tcggggagac ctcacgccga ttggcgggtc tgccagaaac
     3661 tcaccacccg ctcggccgtc gaaggcatca ggcgtccgaa attcacgcgc gcgctctcga
     3721 tatccgcggg cgatggcacc cgattgggac ccaaccgaag caggatcagg gcgcgcacgg
     3781 tggcccattc gaggccgacc gccttgccga cgatcaggat cggatcgtga cggtcgccca
     3841 tgatcagacg gtcgagggct gcgattttca ccgccgacat ggccgaaagc gcggcaatcg
     3901 attcctcgta tttgtaggcc ttggcaaagc caagcagcgc gctttggttc agttcaccgg
     3961 cgtgatgcag cgcgaggatg gtgcgctgcg ccggcgcaaa atcgcggccg ctctcgaccg
     4021 gctcggcctc gccggaaatc tcactcatcg ccatcttgat atcggcctgc cggccgggct
     4081 tgaccacctc gaacaggcgg cggcggacga tatcgattga tcccgccagc aggtccctca
     4141 attgctggct ggagagatcg tcgcgcttgc caatcgcgag cgtcaggacg ccgtcctggc
     4201 cggcacgcct gatcagcgcc gaataaccgg attgggaaaa aagcgccccc gcattgccgg
     4261 cggcgcgcct gaccgccgcg cggtcgccgc ggcgtatgat gacgtcggtg agatcgcagg
     4321 aaagcgtcgg acgctccgac atcgccagca gatggccctg gcccttgatg cgcgcgattt
     4381 cgatcaaggc ccgttcatcg atgacgggcg atcggcgaag caagggacct gcgattgcga
     4441 tctcgtcctc gcgcgcgagg tgcccgacca gggcgcgggg ggcgttggcg aggagggaca
     4501 gccgttcggc cagatcggcc cgcgcggcga gttcggtatg cggaacgaga tcgatcaaaa
     4561 cgccgtcaaa caagtcgacg tgatccgacc ggaaagttgc cgcgccctgc aggaacagct
     4621 cggtgatccg tcgggcggcc tcggcgcggc gcctgggatc gccgtgcctg acgatttcat
     4681 cgagctcggg gatcagcgat ctggcaattg tcatcaaacc cactcaaacc ccagcaaacc
     4741 ccaagccatc tgcgagtttt cagccaattt agatgggctt ggtgaaggaa gggttagatt
     4801 tgaattaagg ccaaacgagc cgcaaattca ttcgcccggg cgtcgcgggc cttgccggat
     4861 agcgcagaaa ccgttatatc aacgctaact tttggttctc atacgatcgc gtattgagag
     4921 tgggtccctt gggacccgct cttttttatt acccgcacga gcctgacgca gatgaacggc
     4981 gccagcccgc tcgggtagcc tagccggacc gccctgaagg cccggctcca acccaacgca
     5041 agttagacca actttgaccc tggatatgac tgatccaact gctggttccg tggacaccga
     5101 tctgcttgcc gagccgcgtc tggtggtcga gcccggcatg gcggcgcggg tgtcggcggt
     5161 cgcagggccg gtgctgcagg gaatgggcta ccggctggtg cgcatcaaaa tatccggaga
     5221 attcggctgc accgtccaga tcatggcgga acggcccgat ggatcgatgc agatcgacga
     5281 ttgcgaggcg atctcgaggg cgctgtcgcc ggtgctggac gtcgccgatc cgatcgaccg
     5341 ggcctaccgg ctggagattt cctcaccggg aatcgaccgg ccgctggtgc gccgttccga
     5401 tttcgaacgc tatgccggcc atctcgtgaa gatcgaaatg gccgccgcgc atcaaggccg
     5461 caaacggttt cgcggcaccc ttggcggcgt cgagggcgat gccgtgcact tacatcgtga
     5521 tgacatacgc gccggcgaag atgccgatgt tgtgctggtg atggaagata tcgccgacgc
     5581 gagactggtt ctgaccgacg agctgatcgc cgaatcgatg cgacgcggca aggcggcgga
     5641 gcgagagctc aagcagagcc tcgggctcgc accaccgcca ccgccctatg ccaggaagag
     5701 cgaccccgcc aggagcaaca agccgaagtt gaaaccaaag ccggaaaaaa agagcgcccc
     5761 gaaaaatacc aaggaacacc gtctcgccgc ggaaagacgg cgtcgaggcg agttcgatcc
     5821 cactgaagga gactgagcca tggcagctgt cagcgccaat aaactcgaac tgctgcagat
     5881 cgcggacgcg gttgcgcggg aaaaatcgat cgaccgcggc atcgtgatcg cggcgatgga
     5941 ggacgccatc gccaaggccg cgcgg
//
LOCUS       DASWGM010000863         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2713273,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000863 DASWGM010000000
VERSION     DASWGM010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2713273"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VGA15_23530"
     CDS             <1..292
                     /locus_tag="VGA15_23530"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018898354.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX9590700.1"
                     /translation="EKLFERSGMKKSDIDLFELNEAFASVVLRYIQAFDIDNAKINVN
                     GGAIALGHPLGATGAMILGTVLDELERTNKSTALITLCIGGGMGTATIIERV"
     gene            439..>2121
                     /locus_tag="VGA15_23535"
     CDS             439..>2121
                     /locus_tag="VGA15_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9590701.1"
                     /translation="MAFKNFKVETDADGIALVTWDIPGRSMNVLDETTITELEAIVKQ
                     TSADAAVKGVVVTSGKEAFSAGADLSMLEGMNHRYTELLKAKGEVAANQMLFDESRKS
                     SLVFRAIETSGKPWVAAINGLALGGAFELTLSCHYRVAAENPKTRLGLPEVKVGLFPG
                     AGGTQRVPRIVPPQDAMQLLLKGEAVNLEKAKALKLIDAVVPAGDLIKAAKDWIKGGG
                     KSVAPWDEKGFKLPGGPVYSKAGMMMFPAGNAIFRRETYDNYPAARAIMSCVYEGLLL
                     PIDAALRVESRYFTQILRSKEAAAMIRSLFLSMQELNKGARRPQNVPPTKVKKLAVIG
                     AGFMGASVGYVSARAGIEVVLIDRDQESADKGKGHAKAVIDDLIKKGRAKEGDRDAIL
                     SRISATADYNVLKDCDLIIEAVFEDRKVKAETYAKAQPLLKSDAIFASNTSTLPINSL
                     AEEFRDQSKFIGIHFFSPVEKMMLVEIIVGKNTGDVALATALDYVRAIGKTPIVVNDS
                     RGFFANRCVLRFTAEGLEMLMEGVPPAMIENTAKMAGMPVGPLSLSDEVALDL"
BASE COUNT          445 a          644 c          675 g          357 t
ORIGIN      
        1 tgaaaagctg ttcgagcgct ccggcatgaa gaaatccgac atcgacctgt tcgaactgaa
       61 cgaggcgttc gcctcggtgg tgctgcgcta tatccaggcc ttcgacattg acaacgccaa
      121 gatcaacgtc aatggcggcg ccatcgcgct cggccatccg ctcggcgcta ccggtgcgat
      181 gattctcggc accgtgctcg acgaactcga gcgcaccaac aaatcgaccg cgctgatcac
      241 gctgtgcatc ggcggcggca tgggcaccgc gaccatcatc gagcgggtgt gaggaactgc
      301 gacgaagcgt catcctgagg tgcgagggcg aagcccgagc ctcgaaggat gaataatttc
      361 atcgggtcat ccttcgaggg cctcgcaaag ggcgaggcca cctcaggatg acggaaacgg
      421 gaattgggag caaacacaat ggccttcaag aatttcaaag tcgagaccga cgccgacggc
      481 attgcgctgg tgacatggga cattccgggc cgttcgatga acgtgctcga cgagaccacg
      541 attaccgagc ttgaagcgat cgtgaagcag acctccgccg acgcggcggt gaagggcgtc
      601 gtcgtcacct cgggcaagga ggcgttctcc gccggcgccg atctctcgat gctcgaaggc
      661 atgaaccacc gatacaccga actgctcaag gcaaaaggcg aggtcgcggc caaccagatg
      721 ctgttcgacg agagccgcaa atcctcgctg gtgtttcggg cgatcgagac tagcggcaag
      781 ccatgggtgg ccgcgatcaa cgggctcgcg ctcggcggcg cgttcgagct cacactgtcg
      841 tgccactacc gcgtcgccgc ggaaaatccg aaaacccgtc tggggctccc ggaagtcaag
      901 gtcgggttgt ttcccggcgc cggcggcacc cagcgcgtgc cacgtatcgt gccgccgcag
      961 gatgcgatgc aacttctgct caagggcgag gcggtcaatc tcgagaaagc caaggcgctg
     1021 aagctgatcg acgccgtggt gccggccggc gatctcatca aggccgcgaa ggattggatc
     1081 aagggcggcg gcaagtccgt cgcgccctgg gacgagaagg gtttcaagct gccgggcggc
     1141 cccgtctact ccaaggccgg catgatgatg ttcccggcgg gaaatgcgat cttccgccgc
     1201 gagacctatg acaactatcc ggcggcgcgc gccatcatga gctgcgtcta cgagggattg
     1261 ctgttgccga tcgacgcagc tttgcgcgtt gaatcccgct acttcacgca gatcctgcgc
     1321 agcaaggaag ccgcggcgat gatccgcagc ctgttcctgt cgatgcagga actgaacaag
     1381 ggcgcgcgcc gtccgcaaaa cgtgccgccg accaaagtga aaaaactcgc cgtcatcggc
     1441 gccggcttca tgggcgccag tgtcggttac gtctcggcgc gtgccggcat cgaggtggtg
     1501 ctgatcgacc gcgaccagga gagcgccgac aagggcaagg gccacgccaa ggccgtcatc
     1561 gacgatttga tcaagaaggg ccgcgccaag gaaggcgatc gcgacgccat tctgtcccgc
     1621 atcagtgcga ccgccgacta caacgtattg aaggactgcg atctgatcat cgaagcggtg
     1681 ttcgaggacc gcaaggtcaa ggcggagacc tacgccaagg cgcagccgct gctgaaatcc
     1741 gacgcgatct ttgcgtcaaa cacctcgacc ttgccgatca attcgctggc ggaggaattc
     1801 agggatcaga gcaagttcat cggcattcac ttcttttcgc cggtcgaaaa gatgatgctg
     1861 gtggagatca tcgtcggcaa gaataccggc gacgtcgcac tcgcgaccgc gctggactac
     1921 gtacgcgcga tcggcaagac gccgatcgtg gtcaatgatt cccgcggctt cttcgccaac
     1981 cgctgcgtgc tgcgcttcac cgccgaaggc ctggaaatgc tgatggaagg cgtgccgccg
     2041 gcgatgatcg agaacaccgc gaaaatggcg ggcatgccgg tcggaccgct gtcgctgtcc
     2101 gacgaggtcg cgctcgatct c
//
LOCUS       DASWGM010000864         4168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_115364,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000864 DASWGM010000000
VERSION     DASWGM010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115364"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..423)
                     /locus_tag="VGA15_23540"
     CDS             complement(43..423)
                     /locus_tag="VGA15_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9590702.1"
                     /translation="MSKTEPNHAPFSYDGLDRVIHEKARLSLLTSLIAHPKGLAFADL
                     KQLCGLTDGNLSRHLQVLQEAGLVEVTKGYEGNRPHTSCRLTKIGRRRFLDYLAVLER
                     LVRDAAKAAGKDETASARLGILPA"
     gene            complement(410..1027)
                     /locus_tag="VGA15_23545"
     CDS             complement(410..1027)
                     /locus_tag="VGA15_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590703.1"
                     /translation="MRDLDKALADIFAIRSQIAAGTAFRGYGPATVAATGGLALATAL
                     IQFWWLDDPTSRPLTFFSGWTATALVSGAIIWIEMQARSRRHHSGLADAMIHQAIEQF
                     LPAGMAAALLAVMLWKFAPETLWMLPGLWQLLVGLGIFASVRSLPRTVGFAGAWYFVV
                     GFTVLLLASQSHALSPWTMGLPFVAGQLLMAALLHFASGETDVED"
     gene            complement(1118..2350)
                     /locus_tag="VGA15_23550"
     CDS             complement(1118..2350)
                     /locus_tag="VGA15_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9590704.1"
                     /translation="MQRFHHEGLHAKAVKAAIIAAGLIVATASFAEDRKYDPGASDTE
                     IRIGQTMAYSGPVSAFAALGTVQAAYFRMINDRGGINGRKINLISYDDAATPSKTVEQ
                     VRKLVEGDEVLFTFQTLGNASNIAIQKYLNDRKIPQLFVAGRATRFENPAEFPWTMGF
                     APNLRTEISVYARFILDNHPNARIGLLYPNDDGGKDYLAGLKQALGARAAAMLVSTAS
                     YEPTDPTVDSQVVLLKTAGVDLLVNMASPKFAAQTIRKAAELDWKPVHLLGVGSSSIG
                     AVLTPAGLDNSKGLISASSFKEAGDPSWTNDEGMKKWVAFMDAYYPEGDRKSIFTTYG
                     YSAAELMVQVLRQCGDDVSRENVMRQAANLKNFRLDLLLPGLAINTSPSDYRVVKEFR
                     MMRFNGERWEPFGPIVAD"
     gene            complement(2470..3351)
                     /locus_tag="VGA15_23555"
     CDS             complement(2470..3351)
                     /locus_tag="VGA15_23555"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9590705.1"
                     /translation="MVNPVERLTAEWQLAPAAALSDLPYTGMAVMRWTCRTALKREFV
                     SRVGDDVHVFSYVLRNSRATSWLDGRQVWDGLIPAHGLRILAPSSQPRWFAHSAFDSM
                     QFHVPVRALREMVDGGRLGGARIVLQDPLYASDPGVTPLVHAMATALASQDQLTPAYI
                     DGLAIALLTRLVMRFSLSGFGEQRPATALDGRLRRAIDFINAHLADDLRLTTMSSVAG
                     LSPYHFSHLFTAAMGVPPHRYLLKLRIERAKQRLLFSREPILTIALDCGFKDASHFAR
                     VFAGETGLSPRRFRQAA"
     gene            3554..>4168
                     /locus_tag="VGA15_23560"
     CDS             3554..>4168
                     /locus_tag="VGA15_23560"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEX9590706.1"
                     /translation="MTDQADLLTQFSNALVTRAEAVKSAVVAVRLRHERHITGMVWRP
                     DVVVVSEQSLPRKDDFELVAAGGSVLAAKIAGRDPATNIAVLRLAGSIASPSIAAGEA
                     QTGAVALAIGADGTGGASARLGLVNLAGAEWHSSRGGLIDRRIVLDLSLGRREEGGPV
                     RDAAGACLGMSTFGPRGQVIAIPTATIERIVPLLLKDGHIARGWL"
BASE COUNT          804 a         1313 c         1322 g          729 t
ORIGIN      
        1 actttgcatc acaaagtttc cttgcaaaaa aagctgtgca gatcaggcgg gcaggatacc
       61 gagccgggcg gaagcggttt cgtccttgcc cgcggccttg gccgcgtcac gaaccagccg
      121 ctcgagcacg gccaaatagt cgagaaagcg gcggcggccg attttcgtca ggcggcaact
      181 ggtgtgcggc cggttgccct catagccctt ggtgacttcg acgaggcctg cttcctgcag
      241 cacctgaaga tgacgactga gattgccgtc ggtcagcccg cagagctgct tcagatcggc
      301 gaaagccagt cccttgggat gcgcgatcag cgaggtcagc aggctgagcc tggccttctc
      361 gtgaatgacg cgatcgagac cgtcatagga gaacggcgcg tgattcggtt cagtcttcga
      421 catcggtttc tccggaagca aaatgcagca gggcggccat cagtaactgg ccggccacga
      481 acggcaggcc catcgtccat ggcgacagcg cgtggctttg gcttgccagc agcaacaccg
      541 tgaagccgac cacgaaatac caggcgccgg caaagccgac ggtgcgcggc aacgatcgga
      601 cggaagcgaa tatcccgagc cccaccagca gttgccatag accggggagc atccacagcg
      661 tctccggagc gaacttccag agcatcacgg ccagcagggc ggctgccatg cccgccggca
      721 gaaactgttc gatcgcctgg tggatcatgg cgtcggcgag acccgaatga tgccgccgcg
      781 agcgcgcctg catctcgatc cagatgatgg ccccggacac caaggcggtt gcggtccagc
      841 ccgagaagaa ggtcagcgga cggctggtcg gatcgtcaag ccaccagaat tgaatgagcg
      901 ccgtggcgag ggccagcccg ccagtggcgg cgacggtcgc cggaccatag ccgcgaaacg
      961 ccgtgccggc ggcaatctgg ctacgaatgg caaaaatatc ggccagcgcc ttgtcgagat
     1021 cgcgcatttc cttccccaac tttgtaatac aaagtatacc cggttgcaaa gtggacgcaa
     1081 gtcccgccgg atgattgtac ggctaaccga cgggcagcta gtcggccacg atcggcccga
     1141 acggctccca tcgctcgccg ttaaaacgca tcatccggaa ctccttgacg acgcgatagt
     1201 cggacgggct ggtgttgatc gcaagaccgg gcaacagcag atccagcctg aaattcttca
     1261 ggtttgccgc ctgccgcatg acattctcgc gcgatacgtc gtcgccgcat tgccgcagca
     1321 cctgcaccat caattcggcc gcgctgtagc cataggtcgt gaagatgctc ttgcgatcgc
     1381 cttcgggata gtaagcgtcc atgaacgcga cccatttttt catgccttcg tcgttggtcc
     1441 aggacgggtc gccggcctcc ttgaacgaac tcgccgagat cagtcctttg gaattgtcaa
     1501 gacccgcggg cgtcagaacg gcgccgatcg aagacgagcc gacgccgaga agatgaaccg
     1561 gtttccagtc gagttctgcc gccttcctga tcgtctgagc cgcgaatttc ggcgacgcca
     1621 tattcaccag gagatcgaca cccgccgtct tcaacagcac gacctgcgag tcgaccgtcg
     1681 gatccgtcgg ctcgtatgaa gcggtactga cgagcatcgc agccgccctt gcgccgagtg
     1741 cctgcttcaa accggcgaga tagtccttgc caccgtcgtc attcggatag agcaggccaa
     1801 ttctggcgtt cggatggttg tccaggatga agcgcgcgta gacgctgatc tcggtccgca
     1861 gatttggggc aaatcccatc gtccagggaa attctgccgg gttctcgaac cgcgtcgcgc
     1921 gcccggccac gaacagctga gggatcttgc gatcgttgag gtatttctga atggcaatgt
     1981 tggaggcgtt gccaagcgtc tggaacgtga acagcacttc gtcgccctcg acaagcttgc
     2041 gaacctgctc gaccgtcttg gacggcgtcg ccgcgtcatc gtacgaaatc aggttgatct
     2101 tgcgtccgtt gatgccgccg cgatcattga tcattcggaa ataggccgcc tgaaccgtgc
     2161 cgagtgccgc gaacgccgag accgggccgc tgtaagccat ggtctggccg atcctgattt
     2221 cggtatcgct cgcgccggga tcgtattttc ggtcttccgc gaacgaagcg gttgcgacaa
     2281 taagtccggc cgcaatgatc gctgccttca ccgccttcgc gtgcaggccc tcgtgatgga
     2341 accgctgcat tgccacggcg cacattctcc atcggtactt aggactccgc aaacacattg
     2401 tctagcgctg cgagctgcca agctctcgcc cattcttgcg gaacaggccg gatcttgcgg
     2461 cgtgcgaatt caagccgctt gccggaaccg ccgcggagac aggccggttt cacctgcgaa
     2521 gacccgcgcg aagtggctcg cgtccttgaa gccacaatcg agcgcaatgg tgagaatggg
     2581 ctcgcggctg aacagcagcc gctgcttggc gcgctcgatg cgcagcttga ggagatagcg
     2641 gtgcggcggc acgcccatgg cggcagtgaa cagatgcgag aaatgatacg ggctaaggcc
     2701 cgcgacggaa gacattgtcg tgagcctgag atcatctgcc agatgcgcgt tgatgaaatc
     2761 gatggcgcgg cgcagccgcc cgtcgagagc cgtcgcgggc cgctgctcgc cgaagcctga
     2821 caaggaaaag cgcatgacaa gccgcgtcag aagcgcgatc gcgagaccgt cgatgtaagc
     2881 cggggtaagc tggtcctggc tggcgagcgc cgtcgccatg gcgtggacaa gcggcgtcac
     2941 gccggggtcg ctggcataga gcgggtcttg caggacgatc cgcgcgccac ccaaccggcc
     3001 gccgtcgacc atctcgcgca acgcgcggac cggaacatga aactgcatcg agtcgaacgc
     3061 gctgtgggcg aaccagcgcg gctgcgacga cggcgccagg atccgcaggc catgtgccgg
     3121 gatcaggccg tcccagacct gtcgtccatc cagccatgag gtcgcgcgcg agttgcgcag
     3181 cacataggag aagacatgca cgtcgtcgcc gacgcgcgag acgaattcgc gtttgagcgc
     3241 cgtacggcag gtccagcgca tgacggccat gccggtatat ggcaaatcgg acagcgcggc
     3301 ggccggggcg agctgccact ccgcggtaag ccgctcgaca ggatttacca ttgcttgctc
     3361 ccacgttctg gcccccgaca tcgagacgac ccggcgcggc aacggccgct gcgttcacgc
     3421 tggcggcgtt caggcccaca tcgggttttg cagcccggtc aaatggccgg gatgaaccca
     3481 gccgtctggc cgatagtttg gacctgcgca atcgcccagt ttcgtcccta tcttaggcgg
     3541 ataggagtta cccatgaccg atcaagcaga cctgttgacc cagttttcga acgccctggt
     3601 aacccgggcg gaagcggtga aaagcgccgt cgtcgcggtc cgcctccgcc acgagcggca
     3661 tatcacgggc atggtgtggc gaccggatgt tgtcgtcgtc tccgagcagt cgctgccgcg
     3721 aaaagatgat tttgaacttg tcgcggccgg cggctcggtc ctcgcggcca aaattgccgg
     3781 acgcgatccc gccaccaata tcgcggttct gcggctggca gggtcgattg cgtcgccatc
     3841 gatcgccgcg ggcgaggcgc agaccggcgc ggttgccctg gcgatcgggg ccgatggaac
     3901 cggcggcgcg agcgcgcggc tcgggctcgt caatctcgcc ggcgccgaat ggcattccag
     3961 ccgcggcggc ctgatcgacc ggcgcatcgt gctcgatctt agcctcgggc gccgcgagga
     4021 gggcggaccg gttcgcgacg ccgccggcgc atgtctgggg atgtcgacct tcggaccacg
     4081 cggccaggtc atcgccattc cgaccgcaac catcgaacgc atcgtaccgc tgctgctcaa
     4141 ggacggccac atcgcgcgcg gctggctc
//
LOCUS       DASWGM010000865         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_115447,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000865 DASWGM010000000
VERSION     DASWGM010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115447"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VGA15_23565"
     CDS             complement(<1..990)
                     /locus_tag="VGA15_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX9590707.1"
                     /translation="MNAPERNLSAPIDPVKLDRLAEVAIKVGLQLKLGQDLLVTAPTV
                     ALPLVRKIAEYAYKAGAGLVTPILSDEAITLSRYRFGHDVSFDRAAGWLYEGMSKAFA
                     ANTARLAIVGDNPMLLSGEDPAKVARASKANSIAYQPALERIVGFDINWNIIAYPSPS
                     WAKQVFPGDEEDVAVAKLADAIFAASRVDNDDAIAAWTKHNAMLRSRTEWLNGQRFSA
                     LHYSGPGTELTIGLADGHEWEGGASTAKNGITCNANIPTEEVFTTPHARRVDGYVVST
                     KPLSYQGSLIDEIAVRFEEGRIVEATASRGEEVLNKVLDTDEGARRLGEVALVP"
     gene            complement(1082..1885)
                     /locus_tag="VGA15_23570"
     CDS             complement(1082..1885)
                     /locus_tag="VGA15_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9590708.1"
                     /translation="MPKYLCKACGIQHPERSGPPERCLICEDPRQFVPTAGQEWITPK
                     TLAVGRFNVFRKVAPGLFGISTVPQFAIGQRAFLVVTPAGNILWDCLSFLDDATIEII
                     RALGGIKAVAVSHPHFYSAMATWGRTFDCPVFVHDADRQWLVEPDPSVKFWTGETRDV
                     LPDITLHRLGGHFPGSTVLHLPERRTLLSGDTMLVTWDQMHVSFMWSYPNYVPLTAAE
                     VERIGRRLAALDFDAIYSAFWGRGDIEHNAKAAVERSIARHILGPGAPV"
BASE COUNT          438 a          691 c          716 g          445 t
ORIGIN      
        1 cggcaccagc gccacttcgc cgagccggcg cgcgccttcg tcggtatcca gcaccttgtt
       61 gaggacctct tcgccgcgcg atgccgttgc ctcgacgatg cggccttcct cgaaccgcac
      121 cgcgatctcg tcgatcagcg aaccctgata ggatagcggc ttggtgctga cgacataccc
      181 gtcgacccgc cttgcatgcg gcgtggtgaa tacttcctcg gtcgggatat tggcgttgca
      241 ggtgatgccg ttcttggcgg tcgaggcgcc gccttcccat tcatggccat cggcaagccc
      301 gatcgtcagc tcggtgccgg gcccggaata atgcagcgcg ctgaaccgct gaccgttcag
      361 ccattcggtc cggctgcgca gcatggcgtt atgcttcgtc cacgcggcga tggcgtcatc
      421 gttatcgacc cgcgaggccg cgaagatcgc gtcggccagt tttgcgaccg cgacatcctc
      481 ctcgtcaccc gggaaaacct gcttcgccca ggacgggctc ggataggcga tgatgttcca
      541 gttgatgtcg aaaccgacga tcctttccag cgccggttga taggcaatcg aattggcttt
      601 gctggcgcgc gcgaccttgg cggggtcttc accggaaagc agcatggggt tgtcgccgac
      661 gatcgcgagc cgcgcggtat tggcggcaaa cgccttcgac attccctcat agagccagcc
      721 ggccgcgcgg tcgaagctga cgtcgtggcc gaaccggtag cgcgacaacg tgatcgcctc
      781 gtcggacagg atcggcgtaa caaggcctgc tccggccttg taggcgtact cggcgatctt
      841 gcgtaccagc ggcagcgcca ccgtcggcgc cgttaccaag aggtcctgtc ccagtttcag
      901 ctgcagccca accttgatgg ccacctcggc gagacggtcg agttttaccg gatcgatcgg
      961 tgccgaaagg ttgcgttcgg gtgcgttcat gaagtccggc ctgttggttg tggttggaat
     1021 tataaagtga ttcatgcggg gcagatcaag gtccccgcgc gcctggcgcg ggtgcgcacc
     1081 ttcagaccgg cgctcccggc cccaggatgt ggcgggcgat ggaccgttcg acggcagcct
     1141 tggcgttgtg ctcgatgtcg ccgcgtcccc aaaatgcaga atagatcgcg tcgaaatcaa
     1201 gcgcggccag gcgccgtccg atgcgctcca cttccgcggc tgtcaaaggg acgtagttgg
     1261 gataggacca catgaaagag acgtgcatct ggtcccacgt caccagcatg gtgtccccgg
     1321 atagcagggt gcgacgctcg ggaagatgca aaaccgtgct gccaggaaaa tggccgccga
     1381 gccgatgaag cgtgatatca ggcaaaacat cccgcgtctc tccggtccag aattttacgc
     1441 tcggatcggg ctcgacaagc cattgccggt cggcatcgtg cacgaacaca ggacagtcaa
     1501 acgtccggcc ccacgtcgcc atcgcgctgt agaaatgcgg atgggaaaca gcaaccgcct
     1561 tgattccacc caacgcgcga atgatctcga tggtcgcgtc atccaaaaag ctgaggcagt
     1621 cccaaaggat gttgcccgcg ggggtaacga ccaggaaggc ccgctgtccg atcgcgaatt
     1681 gtggcaccgt ggaaattcca aacaggccgg gagccacttt acgaaacacg ttgaagcggc
     1741 cgaccgccag cgttttcggg gtgatccatt cctgacctgc ggttggaacg aattgccgcg
     1801 gatcctcgca gatcaggcaa cgttcgggtg gcccagaacg ttccggatgc tggatgccgc
     1861 aagctttgca tagatatttt ggcacagcgt atttcccctg gaatgggcgg ttactaccaa
     1921 ttctcgtgat tgtctctcat cgatgcctgc gcgccaagag gcggatggag ggcagcaacg
     1981 ggaacgcggg cttgacattg aggcttttcg cagtcgtcgg ccaatcggag gcgtctagtc
     2041 aggcgcgcgc atgatccagt tcaattccag acatcggaga tagacatatg tccgcgttct
     2101 cgcggcgcca tgcgtccgag gtttggccga acttttgtcc ctctgatgac agagggcgca
     2161 gggaatgccg ggcgcccgat gcgcccgctg ccgcgtgtgc catgatagtg gtagaacgca
     2221 cacgggtaac aacgagtaca ccggaatcgc ccggcattcc cgcacgcaat ggttttaacg
     2281 gtagagtagg
//
LOCUS       DASWGM010000866         6892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_218730,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000866 DASWGM010000000
VERSION     DASWGM010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218730"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VGA15_23575"
     CDS             complement(<1..243)
                     /locus_tag="VGA15_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HEX9590709.1"
                     /translation="MSKVGTSDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREENKSSLVKSRDGIPTPKEICKVLDDYVIGQS"
     gene            complement(659..1291)
                     /locus_tag="VGA15_23580"
     CDS             complement(659..1291)
                     /locus_tag="VGA15_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX9590710.1"
                     /translation="MRDPVETYMQLVPMVVEQTNRGERAYDIFSRLLKERIIFVTGPV
                     EDGMSTLVVAQLLFLEADNPKKEISMYINSPGGVVTSGLAIYDTMQFIRPAVSTLCTG
                     QAASMGSLLLAAGQKDMRFSLPNSRIMVHQPSGGFQGQATDIMLHAQEILNLKKRLNE
                     IYVKHTGQTFKAIEDALERDKFLTAEMARDFGIVDKVIDKRSEDPMAKSP"
     gene            complement(1576..2937)
                     /gene="tig"
                     /locus_tag="VGA15_23585"
     CDS             complement(1576..2937)
                     /gene="tig"
                     /locus_tag="VGA15_23585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX9590711.1"
                     /translation="MQVTETLAEGLKHEFQISVPASDLDAKADVRLVELKYKVRLNGF
                     RPGKVPVSHLKKVYGRSVMAETIDQTIRDTNSQIFTERGFRLATEPKVTMPTEQKAVE
                     DILTGKSDLTYTVSIEVVPTIALADFKSFTVEKPVVEVTDADIDEAIKRIADQNRSFA
                     AKGDGAKAETGDRVTISFKGTIDGTPFDGGTGENIQVTIGSNTFIPGFEEQLLGVAAG
                     ETRVLKVSFPKNYASEKLAGQPAEFETTATLIEAPQETAINDEFAKTLGLESLDKLKE
                     AARERLVAEFAGATRQRVKRMLLDRLDESHRFEAPPSLVEEEFNLMWNSIKAEMESGG
                     KTFADEDTTEEAAREEYHKIADRRVRLGLVLSEIGEKNKITVTDDEVRRAVIERARQM
                     PGREKEVWDYCRNNNNALAQLRAPIYEDKVVDFILELANVTEKKVTREDLYKDDEAEK
                     SAA"
     gene            complement(3111..3195)
                     /locus_tag="VGA15_23590"
     tRNA            complement(3111..3195)
                     /locus_tag="VGA15_23590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3159..3161),aa:Leu,seq:tag)
     gene            4643..5959
                     /locus_tag="VGA15_23595"
     CDS             4643..5959
                     /locus_tag="VGA15_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX9590712.1"
                     /translation="MADRIFQLDRRELIAGLGGAALGPALPSLANAEPLPTLALRAKT
                     DNRPLHPPDVDALLWSIRDTTTDDVGRIKRGDGLGFAFLNELSVPAELNWHGLEGHPE
                     SLFARPGLAAGDVQIFQIPFHQAGTFLCDLRLLGDGQARPSRALALVVEESAPVAVDR
                     DQVFLIEDWRLRPDGTAIAPGIDPKDTVPVYTINGLSTLDIPARANERLRFRFVNGCQ
                     RSVIAIKIEGHEVRVMAIDGQPAEPFPARNGSVVLAPGTRVDAFIDATARPGSTSSIL
                     LHDGKEPRLIARLIASNEPPIRAAPLPLAPPLPSNGLPAQLDLKNALRFDMALGGPQT
                     DWVRPATFAVAAPPSFRAKAGRTVVLALTNRAEIATVFHLHGHHFRLLDRLDDGWKPF
                     WLDTLAVEPGQTQRIAFAAEHAGRWLIESMTTDWAAPRLVRWYSVE"
     gene            complement(6107..>6892)
                     /locus_tag="VGA15_23600"
     CDS             complement(6107..>6892)
                     /locus_tag="VGA15_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX9590713.1"
                     /translation="AVIVSGDIAATGAARLSARGALRAGAGLVTLASPRDALAVNAAA
                     LTAVMVRAIDTVVEFGELLTDKRLKTCVIGPGAGVGERTRDLVLTALSAKRHLVLDAD
                     ALTSFADAPDRLFEAIRASQDQQVVLTPHEGEFPRLFSDISNKHPYRSKLERVRAAAE
                     RCGGIVLLKGSDTVVASPDGRATISANAPPWLATAGAGDVLAGMIAGLLAQGVPAFEA
                     ASIGAWMHGEAAREAGPGLIAEDLPEVLPAVFRRLYDEFGIDY"
BASE COUNT         1238 a         2246 c         2093 g         1315 t
ORIGIN      
        1 gctctgaccg atcacgtaat cgtcgaggac cttgcagatt tccttggggg tgggaatgcc
       61 gtcgcgcgac ttcaccaaag aggatttgtt ttcctcgcgg atgatatcca tgcacagctc
      121 aacgcattcg tcgcagatga acacggtggg acccgcgatc agtttgcgga cttcgtgctg
      181 gctcttgccg cagaacgagc aatacagcgt gttcttggag tcgctcgtgc cgaccttact
      241 cattcttatc tccgtccgtc cgctcgatcg ccccatcccg gcacgtaccg gcacagaggt
      301 gtagatagag caaaatccgt accaaaaaag accctacgct ccaggtgcca cgcgaatcgc
      361 ggccaactcg aatcccccac gatcataacc gatccaacct agccaaccat gctgacagcc
      421 gactatcaag aattcgctaa ctgggcgaac acccgatgcc cgtgacaata ccgtgatttg
      481 caccgcccag gcgagcccag atcctgaaat cagccaggcg ttgcgcgatt gccacgccgc
      541 caaaccagca cgaaagcgga acttctagcc ccaaacccgg cataacggcg cggttggcgg
      601 ccaaaactgc cggtcacggc ggtcattaac gccggcctaa ccttcgtggc ccgtgatgct
      661 acgggctctt cgccatcggg tcttccgagc gtttgtcgat caccttgtcg acgataccga
      721 agtcccgggc catttcggcg gtaaggaact tgtcgcgttc cagcgcatcc tcgatcgcct
      781 tgaaggtctg gccggtatgt ttcacgtaga tctcgttgag ccgcttcttg aggttgagga
      841 tctcctgcgc gtgcaacatg atgtcggtcg cctggccctg aaagccgccg gagggctggt
      901 gcaccatgat gcgggaattc ggcaacgaga agcgcatgtc cttctggccc gcggccagca
      961 gcagcgagcc catcgacgcc gcctgtccgg tgcacagggt cgacaccgcc ggacggatga
     1021 attgcatggt gtcgtagatc gcaagacccg aggtcaccac tccaccgggg gagttgatgt
     1081 acatcgaaat ttccttcttc ggattatcgg cttcgagaaa cagcagttgc gcgaccacca
     1141 aggtcgacat gccgtcctcg acgggtccgg tcacgaagat gatgcgctct ttcagcaggc
     1201 gggaaaaaat gtcgtaggcc cgttcgccgc ggttggtctg ctcgaccacc atcggcacga
     1261 gttgcatgta ggtttccacc ggatcgcgca ttagtcaccc aagggttggc ggaggcgaac
     1321 atccatcggc aaggaaacta gcaaggctgg gctggctaga atttgccggg cggcggacga
     1381 acgtcccgtg atgcaggagt taaaagacag ggctcacaga tatgagccaa ttccccggcg
     1441 acaaggccaa gtcgatacca agccaacatc gtgcgaagtc attccagttc aagctgattc
     1501 tcaagcccgc gcatagcgac ggatactgcc gccatgcccg tttcgccgca taaccttagc
     1561 gccgggccga tgtcgtcagg cagcgctctt ctcggcctcg tcgtccttgt agagatcctc
     1621 gcgcgtgacc ttcttttcgg tcacgttggc gagttcaagg atgaaatcga ccaccttgtc
     1681 ctcatagatc ggcgcacgaa gctgggccag cgcgttgtta ttgttgcgac agtagtccca
     1741 gacctccttc tcgcgaccgg gcatctggcg cgcgcgctcg atgaccgcgc gcctgacctc
     1801 gtcgtcggtg accgtgatct tgttcttttc gccgatctcg gacagcacca gaccgagccg
     1861 gacccgacga tcggcaatct tgtggtattc ctccctggca gcctcttccg tggtgtcctc
     1921 atcggcaaac gtcttgccgc cggattccat ttcggccttg atcgagttcc acatcaggtt
     1981 gaattcttcc tcgaccagcg acggcggcgc ctcgaaacga tggctctcgt cgaggcggtc
     2041 cagcagcatg cgcttgaccc gctgacgggt tgcgccggcg aattccgcca caagccgctc
     2101 gcgcgccgct tccttgagct tgtcgagcga ttcgagcccg agggtcttgg cgaactcatc
     2161 gttgatcgca gtctcctgcg gcgcctcgat caacgtcgcg gtggtctcaa actcggccgg
     2221 ctggccggcg agcttctcgc tggcgtagtt cttcggaaac gaaactttca agacgcgggt
     2281 ttcgcccgcc gcgacgccga gcagttgttc ctcgaaaccg ggaatgaagg tgtttgagcc
     2341 gatcgtcacc tggatgttct cgccggtgcc gccatcgaac ggcgtgccgt cgatggtacc
     2401 cttgaaggag atcgtgaccc ggtcgccggt ttcggccttg gcgccgtcgc ccttggcggc
     2461 gaagctgcga ttctggtcgg cgatgcgctt gatggcttcg tcaatgtcgg catcggtcac
     2521 ttcgaccacc ggcttctcga ccgtgaaact cttgaagtcg gcgagcgcaa tcgtgggcac
     2581 gacctcgatc gaaacggtgt aggtgagatc ggattttccg gtgaggatgt cctcgacggc
     2641 tttttgttcc gtgggcatcg tgaccttggg ttcggtcgcc agccggaagc cgcgctcggt
     2701 gaagatttgc gagttggtgt cgcggatggt ctggtcgatg gtctccgcca tcaccgagcg
     2761 gccatatacc ttcttcaggt ggctgaccgg caccttgcct ggacgaaagc cgttgagacg
     2821 gaccttgtac ttgagttcca ccagacggac gtccgccttg gcatcgagat ccgacgcggg
     2881 aacgctgatc tggaattcgt gcttcaatcc ctcagcgagg gtttctgtga cctgcatggc
     2941 gtcaatcttc ttccgctttc gtccggcgtt aagcgccggg acattgtcaa tctggtcgac
     3001 gcgcggcctc aaagcctgcg ccggtggttc ggcggacccc atttcttcgg gcaaccggcc
     3061 cttcaagaga taggtcctcc agtattcatc atgcaaacgc tgtcagcgct tggtgcgggc
     3121 ggagggactc gaacccccac aactttcgtc actggaacct aaatccagcg cgtctaccag
     3181 ttccgccacg cccgctagaa tagcatcatg tccgaccgcg atgccgcggg cggcgggctt
     3241 ataacatggg ccgacctgtt cgcagcaaaa aaatggccgg ttttggagag gcccggcccg
     3301 ggccgccgaa actggggcat atccggcgga aaatggggcg cccgtcgctg atgctgcgga
     3361 ccaagggctg gcgccatcgg cttgcgcccc ggccgggcgg acatggtcgc tggaagggcc
     3421 aatgacgttc ctctcattcc tgacacgact tcgccctcgc gacgcgaagc gcccgaggcg
     3481 ttgctggatc ttccgccctt gtgagggcgt ggggaacgcc gggtgcccgc cgcacccgcg
     3541 gcctcgtgcg cgctttgcat tggtaaaaag cacacgagta aacgagtaca ccggaaacac
     3601 ccggcattcc cgcacgcaat ggttttacga cttatttcgt gctctccccg gtgaccgggc
     3661 tcttttgcca ccgtcgtccg cggattaagg tttcgtccgc gcccggtagg gccgacttga
     3721 cctccgcgaa cttgacgcca gcgtcggggc gtcaggacca cacgacttcg ccgtacgcaa
     3781 caacatcgtt cgtccgctcg ctttgtgatc gctcacaagc cgtgaagcct gccctgcaac
     3841 ctttcgcgcg caaaacgctg ccgcgtccac cgcatccctc cccgcgtcgt gacgatcgcg
     3901 atacgcccct gtggtggggt gggatgcgga aagttgtaga tgtgatttgg ggggtgcgga
     3961 aacagatata tttttgcaaa ggggactcga cacaaaactg cccgacgggc aaatcaccac
     4021 ggcggcgcga gcccctctcg tgtcccggac gcggtgcagc gcccttcgcg ctgctccgca
     4081 gagccgggac ccatatcgac gctgcgagat ggaccccggc tcagcagcgc accgccatag
     4141 cgcgtcgaag acgcgcgtaa acgcgctgaa ggcgctgcgc tgcgtccggg gaacgctgtg
     4201 gccccgccgg aggttcatca gacgtgtctg atgaaggaat gcggcgagag cccgttcctc
     4261 tcgtgttccg gacgcggtgc agcgcttctt cagcgctgct gcgcagagcc gggacccata
     4321 tcgacacttg cgagatggac cccggctcag cagcgcaccg ctgcgcgctg cgctgcgtcc
     4381 ggggaacgct gtggccccgc cggaggttta tcagacgtgt ctgatgaagg aatgcggcga
     4441 gagcccgttc ctctcgtgtt ccggacgcag tgcagcgctt cttcagcgct gctccgcaga
     4501 gccgggacct acagcagtgc gagccgcacg atggaccccg gattgcacgt tgggagcgcc
     4561 ttctggataa gagggcgccg gcgggtcctc gtgacctcgc ggcaactgga catatccggc
     4621 ggaaagtgct ccgcatctga ccatggcaga tcgcattttc cagctggacc gacgtgagct
     4681 gatagccgga ttgggcggcg cggccctcgg tccggcgttg ccttccctgg ccaatgccga
     4741 acccctgcct acgctggcgc ttcgtgccaa aaccgacaac aggccgttgc accccccaga
     4801 cgtcgatgcc ttgctgtggt caatcaggga tacgaccacg gacgacgtcg gccgcatcaa
     4861 acgcggagac ggcctcggat tcgcgttttt gaacgaatta tcggtcccag cagaactcaa
     4921 ttggcacggg ctggagggcc atccggagtc actgttcgcg cgccccggcc tagccgcagg
     4981 ggatgtccag atcttccaaa ttccgttcca tcaggcgggc acgttcctgt gcgaccttcg
     5041 gctactgggt gacggtcaag cgcggccgtc gcgcgccctc gcgctggtcg tcgaggagag
     5101 cgcgcctgtc gccgtcgatc gcgatcaggt gtttctgatc gaagactggc ggctgcgccc
     5161 ggacggaacc gcgatcgcgc ccggcatcga tccaaaggac accgtgcccg tctacaccat
     5221 caacgggctc agcacgctgg atatcccggc gcgcgccaat gaacgcctga ggtttcgctt
     5281 tgttaacggc tgccaacggt cggttatcgc catcaaaata gaggggcacg aggtccgtgt
     5341 catggccatc gacggccagc ccgccgaacc gttcccggcc cgcaacggct ccgtggtgct
     5401 ggcgccgggg acccgggtcg acgccttcat cgacgcgacc gctcggcccg gctcgacctc
     5461 ttcgatcctg ctgcatgacg gcaaagagcc tcgcctcatc gcccggctga tcgcctccaa
     5521 cgagccgccg atccgcgctg cgccgctgcc gctggcaccc ccgctgccat ccaacggcct
     5581 gccggcccag ctcgacctca agaacgcctt gcggttcgat atggcgctcg gcgggccgca
     5641 gaccgactgg gtgaggccgg cgaccttcgc tgtagccgcc ccgccctcgt tccgcgccaa
     5701 ggcgggccgc acggtggtgc tggcgctgac caaccgcgcc gagatcgcga ccgtattcca
     5761 tctccatggc catcatttca ggctgctgga tcggctcgat gacggctgga agccattctg
     5821 gctggatacg ctggcggtcg agcccgggca gacacaacgc atcgcattcg ctgccgagca
     5881 tgccggccgc tggctgatcg agtcgatgac gaccgactgg gcggcgcccc gcctcgtgcg
     5941 gtggtacagt gtcgagtgag gtgcgaacac ctcgcacatg ggcttcgaag ggtccccaaa
     6001 cagaccaatc caacccgaca tgaatgtgtc agctatgtct ccgaacacac cgtcagtgat
     6061 ccgtcggggc taaccatttc gtggacatga cgaggcattt tccgtctcaa tagtcgatgc
     6121 cgaactcatc gtagaggcgc cgaaaaaccg cgggcagcac ttccggcaga tcttccgcga
     6181 tcagaccggg gcccgcttcg cgcgcggcct cgccatgcat ccaggccccg atgctggctg
     6241 cctcgaaggc cggcacgcct tgcgccagca gacctgcgat cattcccgcc agcacatcgc
     6301 cggcgcccgc ggtggcgagc cagggcggcg cgttggcgga gatggtcgcg cgcccgtcag
     6361 gtgatgccac cacggtgtcc gatcccttga gcagaacgat gccgccgcag cgctcggccg
     6421 ctgccctgac gcgttcgagt ttcgagcgat atggatgctt gttgctgata tcgctgaaca
     6481 gccgcggaaa ttctccctca tgcggagtga gaaccacctg ctgatcctgc gatgccctga
     6541 tcgcttcgaa cagacgatcg ggagcatcgg caaaacttgt cagcgcatcc gcatccagca
     6601 cgaggtgccg ctttgccgac agcgccgtca ggacaagatc gcgggtccgc tcgccgacgc
     6661 cggcacccgg cccgatcacg caggttttga ggcgcttgtc ggtcagcagc tcgccaaact
     6721 cgaccaccgt atcgatcgcg cgaaccatca cggcggtcag cgcagcggca ttgacggcga
     6781 gcgcatccct gggcgaggcc agcgtgacca gcccggcgcc ggcccgcagc gccccgcgcg
     6841 ccgacagccg cgccgcaccg gtggcggcga tatcgccgga tacgatgacc gc
//
LOCUS       DASWGM010000867         4703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_218837,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000867 DASWGM010000000
VERSION     DASWGM010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218837"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2185
                     /locus_tag="VGA15_23605"
     CDS             <1..2185
                     /locus_tag="VGA15_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX9590714.1"
                     /translation="GILNLTNYKPPAPDDYYLGQRRMTSEIRDLYGQLIDGMQGTRGQ
                     IKTGGDAAGAELQGSPPTQKPLALYSGIITVAADGSAEISFDIPEFAGTARVMAVAWT
                     ATKLGRATSDVTVRDPVVLTATLPRFLLNGDHGTMSFDLDNVEGAPGDYTINVKTAGP
                     VRVTGNPATTATLSAKQRTSMSLALDAGGAGTANLDVDIKGPNGLTLARHYTLDVKPA
                     TQILARRSIRTLAKGESLTLTSDMFSDLVQGTGGVSLSVSLSTALDAATILKALDRYP
                     HGCSEQITSRAMPLLYVNDLAAGAHLAMDTEVDQRIKDAIDRLLARQGSNGSFGLWSA
                     GGDDAWLDAYVTDFLTRAREKGFAVPDVLFRNALDRIRNSVVNANEPEKDGGRFLAYG
                     LYVLARNGAAPIGDLRYLADTKINNLATPIAKSQLAAALALVGDKGRAERVYAAALES
                     LAPKPVLEFGRTDYGSALRDAAALVSLASEGNAPRATLTQAVARVEVARGLTPYTSTQ
                     ENAWLVLASRALVRETLALDIDGAPVKTALYRSYKAAEMEGKPIKITNTGDAAVQAVI
                     SVSGAPVTPEPAASNGFKIERSYFTLDGKPADVAKAKQNDRFAVVLKITEPKPEYGHI
                     MVSDYLPAGLEIDNPHLVSSGDTGRLDWIEDGKEPENTEFRDDRFTAAIDRAANDKAV
                     FTVAYVVRAVSPGKYVLPQAYVEDMYNPSRYGRSGTGAVEVRPAK"
     gene            2296..4413
                     /gene="pbpC"
                     /locus_tag="VGA15_23610"
     CDS             2296..4413
                     /gene="pbpC"
                     /locus_tag="VGA15_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX9590715.1"
                     /translation="MDGRDKPGHDGVVRRRNLRAVAVTSAIVCAFVTIAMIVWVVSLG
                     PLPLAQARQVSTTIVDRNGKLLRAYAMADGRWRLPVDARHNVDPGYLKLLLAYEDRRF
                     YSHAGVDPFALGRAAFQLVTRGHIVSGGSTITMQLARLLEPRRQRSVYAKLRQMVRAL
                     EIERQLTKDQILDLYLALAPFGGNLEGVRAASIAYFGKEPKRLSLAESALLVALPQSP
                     ENRRLDRYPNVARAARDRVLDRMVEEGRVAAEDAAQAKSVGVSRLRKTMPILAPHSAD
                     QAMATVKDADVIALTLDSGLQKVLEALARDRATALGENISIAIIAVDNESGDVLARVG
                     SPDYFDDRRAGQVDMTRAVRSPGSTLKPFIYGLAFEDGFVHPESLIDDRPIRFGSYAP
                     ENFDMTFQGTVPVRKALQLSLNVPAIALLDRVGASRLSSRLKQAGVNLVLPKDEAPGL
                     AMGLGGVGVTLQDLAQLYSGLARLGSTRPLREIVKATDDPRDSLRLMDQAAAWQVGNV
                     LIGTPPPENGVHNRIAFKTGTSYGYRDAWSVGFDGRITIGVWVGRPDGVPVPGLVGRT
                     AAAPILFDAFARTGKLPAALPKPPKGTLVASNAKLPLPLRRFRPVGELVRTGGEQAPH
                     IQFPLNGSRIYVDRSGVTELSAMPVKVAGGVLPLTMLVNGVSVGEIDSRRQRLVDPPG
                     PGFARLTVIDATGAADTVVIRIQ"
     gene            4465..>4703
                     /locus_tag="VGA15_23615"
     CDS             4465..>4703
                     /locus_tag="VGA15_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX9590716.1"
                     /translation="MVETYARPRFGEPRQPKNRVNPGSRLVGVIDVATASHVRAIAFL
                     LLCGLLLFLPGFFNIPPIDRDEARFAQATKQMVESG"
BASE COUNT          823 a         1478 c         1550 g          852 t
ORIGIN      
        1 cggcattctc aatttgacaa attacaagcc gccggcgccg gacgattact atctcggcca
       61 gcgccgcatg acgtcggaaa tccgcgacct ctatgggcaa ttgatcgacg gcatgcaggg
      121 gacgcgcggc cagatcaaga ccggcggcga tgctgccggc gccgagttgc agggcagtcc
      181 gccgacgcaa aaaccgctgg cgctgtattc cggcattatc acggtggccg ccgacggttc
      241 ggccgaaatc agtttcgata ttcccgagtt cgccggcacc gcccgggtga tggcggtggc
      301 ttggaccgcc acgaaacttg gccgcgccac atcggatgtc acggtgcgcg atccggtggt
      361 gctgacggcg acgctgccgc gtttcctgct caatggcgat cacggcacca tgagttttga
      421 tctcgacaat gtcgaaggtg cgcccggcga ctacaccatt aatgtcaaga cggcgggtcc
      481 tgtgcgggtg actggcaatc ccgccaccac cgcaacactc tcggcaaaac agcgaacctc
      541 gatgtcgctg gcgctcgatg ccggcggcgc cggaacggcg aacctcgatg tcgatatcaa
      601 gggaccgaac gggctgacgt tggcgcggca ttatacgctc gacgtcaagc cggcgacgca
      661 aattctggcg cgccgctcga tccgcacttt ggccaagggc gagagcctga cgctgacatc
      721 cgatatgttt tcggatctgg tgcagggcac cggcggcgtc tcgctgtcgg tcagcctgtc
      781 gacggcgctc gatgccgcga ccatcctgaa ggcgctggac cgttatccgc atgggtgctc
      841 ggagcagatc accagccgcg cgatgccgct gctgtacgtc aatgatctgg cggccggcgc
      901 gcatcttgcg atggacacgg aagtcgatca gcgcatcaag gatgcgatcg atcggctgct
      961 ggcgcggcaa ggctccaacg ggtcgttcgg gctgtggtcg gccggcggtg acgacgcctg
     1021 gctcgacgcc tatgtcacgg actttttgac ccgcgcccgc gaaaagggat ttgcggtgcc
     1081 ggatgtgctg ttcagaaacg cgctcgatcg catccgcaac tcggtcgtga atgccaacga
     1141 gcctgagaag gacggcgggc gctttctcgc ttacggcctc tacgtgctgg cccgcaatgg
     1201 cgcggcaccg atcggcgatc tgcgctacct tgccgatacc aagatcaata acctcgccac
     1261 cccgattgcc aaatcgcagt tggcggcggc gctggcgctg gtcggcgaca agggcagggc
     1321 cgagcgggtc tatgccgcag cgctcgaaag cctggcgcca aagccggtgc tggagttcgg
     1381 ccgaaccgac tacggcagcg cgttgcgcga cgccgcggca ttggtttcgt tggccagcga
     1441 aggcaatgcg ccgcgggcaa cgctgacgca agccgtcgcg cgggtcgaag tcgcgcgcgg
     1501 gctgacgcct tacacctcga cccaggaaaa tgcctggctg gtattggcgt cgcgggcgct
     1561 ggtcagggaa acgctggccc tcgatatcga tggcgcgccg gtcaagaccg cgctgtatcg
     1621 cagctacaag gccgccgaga tggagggcaa gccgatcaag atcaccaaca ccggcgatgc
     1681 ggcggtgcag gcggtgatct cggtgagcgg cgcgccggta acaccggaac cggcggcgtc
     1741 gaacggtttc aagatcgagc gcagttattt taccctcgac ggcaagccgg ccgacgtcgc
     1801 gaaggcgaag cagaatgacc gcttcgccgt cgtgctcaag atcaccgagc ccaagccgga
     1861 atacggtcac atcatggtgt cggattatct tcccgccggc ctcgagatcg acaacccgca
     1921 tctggtgtcg tccggtgaca ccggcaggct ggactggatc gaggatggca aagagccgga
     1981 aaataccgaa ttccgcgacg accgcttcac cgccgccatc gaccgcgctg cgaacgacaa
     2041 ggcggtgttc acggtggcct atgtggtgcg tgcggtatcg ccgggcaaat acgtgctgcc
     2101 gcaagcctat gtcgaggaca tgtacaaccc ctcgcgctat ggccgctccg gcaccggcgc
     2161 cgtcgaggtg cgtccggcga aatgagcgcg ccaggttcac aaaggccttc gtcatgtccg
     2221 ggcaaaaggc gcgaagcgcc gtcttcgcac caagcgtccc gggcatccac gtctgtttca
     2281 cgcaacgaag aagacgtgga tggccgggac aagcccggcc atgacggcgt tgttaggcgg
     2341 cgcaaccttc gcgccgttgc tgttacttcc gcgatcgtct gcgccttcgt caccattgca
     2401 atgattgtgt gggtcgtctc gctgggtcca ttgccgctcg cgcaggcccg gcaagtctcc
     2461 accaccatcg tcgaccgcaa cggcaaactg ttgcgtgcct acgcaatggc cgacggccgc
     2521 tggcggctgc cggtcgatgc caggcacaac gtcgacccgg gctacctcaa attgctgctg
     2581 gcgtatgaag accggcgttt ttattcccac gcgggcgtcg atccctttgc attgggccgc
     2641 gccgcgtttc agcttgtcac gcgcggtcac atcgtgtccg gcggctcgac catcacgatg
     2701 caactggccc ggttgctgga gccgcggcgg cagcggtcgg tttacgccaa gctgcgccag
     2761 atggtccgtg cactcgaaat tgagcggcaa ctgacaaaag atcaaattct cgacctctat
     2821 ctggcgctgg cgccgttcgg cggcaatctc gagggcgtgc gggcggcctc gattgcctat
     2881 ttcggcaagg agccgaagcg tttgtcgctg gctgaatcgg cgctgctggt ggcgttgccg
     2941 cagtcgccgg aaaaccgccg gcttgatcgc tatcccaacg tcgcacgtgc cgcgcgtgac
     3001 cgggttctcg atcgcatggt cgaagagggc cgtgtcgcgg ccgaggatgc ggctcaggcg
     3061 aaatcggttg gggtgtcgcg gctgcgcaag acgatgccga tactcgcgcc gcattcggcc
     3121 gatcaggcga tggccaccgt gaaggatgcc gatgtcatcg cgctgacgct ggattccggt
     3181 ctgcaaaagg ttctcgaagc gctggcgcgc gaccgcgcga ccgccttggg cgaaaatatt
     3241 tccattgcca tcatcgcggt cgacaatgag agcggcgacg tgctggcgcg ggtcggttcg
     3301 cccgactatt tcgatgaccg gcgcgcggga caggtcgata tgacccgcgc ggtgcgttcg
     3361 ccgggctcga cgctcaagcc atttatttat ggcctcgcct tcgaggatgg ctttgttcat
     3421 ccggaaagcc tgatcgacga ccggccgatc cgcttcggct cttacgcgcc ggagaatttc
     3481 gacatgacgt ttcagggaac ggtgccggtg cgcaaggcgc tgcaactgtc gctgaacgtg
     3541 ccggcgatcg cgctgctgga ccgggtcggc gccagccgcc tgtcgtcgcg attgaagcag
     3601 gcgggcgtca atctggtgct gccgaaggac gaagcgcccg gtctcgcgat gggtctcggc
     3661 ggcgtcggcg tcacgctgca ggacctggcg cagctctact caggcttggc gcggctgggc
     3721 agcacccggc cgctgcgcga gatcgtgaaa gcaacagacg acccgcgcga ttcgctgcgg
     3781 ctgatggatc aggctgcggc ctggcaagtc ggcaacgtgc tgatcggcac gccgccgccg
     3841 gaaaatggcg tgcacaaccg gatcgcgttc aagaccggaa ccagctatgg ctatcgcgat
     3901 gcttggtcgg tcggcttcga cggccgcatc acaataggtg tctgggtcgg gcgtcccgat
     3961 ggcgtgccgg ttcccggtct tgtcggccgc accgccgcgg cgccgatttt gttcgatgcc
     4021 ttcgcccgca ccggaaaatt gcccgcagcc ctgccaaaac cgcccaaagg tacgctggtg
     4081 gcgagcaacg ccaaactgcc gttgccgctg cggcggttcc ggccggtcgg ggaactggtc
     4141 cgaaccggcg gcgagcaggc cccccacatc cagtttccgt tgaatggctc gcggatctat
     4201 gtggatcgtt cgggggttac ggaactttcc gcgatgccag tcaaagtcgc cggcggcgtg
     4261 cttccgctga caatgctcgt gaacggtgtc tcggtggggg aaatcgacag ccggcggcag
     4321 cgtctggtcg atccgcccgg ccccggtttt gccagattga ccgtgatcga cgccacgggt
     4381 gccgcggaca cagtcgtcat ccgaatccaa tagaattttg ctagccggtt gcccccgata
     4441 gcggtttcgg cgtatgcgga accgatggtc gagacctatg cccgcccccg atttggagag
     4501 ccacgccagc ctaagaaccg ggtgaacccg ggcagcaggc ttgtcggcgt gatcgatgtc
     4561 gccactgcca gtcatgtccg cgcgattgcg ttcctgctgc tgtgcggtct gctgctgttc
     4621 ctgcccggat ttttcaacat tccgccgatc gaccgcgacg aggcgcggtt cgcgcaggcg
     4681 accaagcaaa tggtcgagag cgg
//
LOCUS       DASWGM010000868         6298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_218882,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000868 DASWGM010000000
VERSION     DASWGM010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218882"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1748
                     /locus_tag="VGA15_23620"
     CDS             153..1748
                     /locus_tag="VGA15_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9590717.1"
                     /translation="MKQSDETWWRHGIFYQVYPRSFQDSNADGVGDIAGLIDRLPYLL
                     ELGIDAVWLSPIFPSPMADFGYDISDYTGVDPLFGTTADFDALISAAHAAGLKLILDL
                     VPNHTSDRHPWFIASRSSRDDGKRDWYIWREPAADGGPPNNWMSEFGGSAWAYDAATR
                     QYYYHAFLAQQPDLNWRNPEVRQAIHDVMRFWLRKGVDGFRVDVIWHLIKDAEFRDNP
                     PNPDFREGQPPHWKVLPLRTTDQPEVHDVIAEMRRVIDEFDARVLIGEIYLPVERLVT
                     YYGKNLAGAHLPFNFALLSAPWNARAIEKIIADYEAVLPAGAWPNWVLGNHDRPRVAS
                     RVGPAQARVAAMLLLTLRGTPTLYYGDEIGMHQVAIAPDQVRDPFEKNVPGIGMGRDG
                     CRTPMQWNATPYAGFSTVAPWLPLSDDFAQDNVVNLDADKRSTLSLYKTLIDLRKKSP
                     QLVSGAYVPIAAQGDLLIYRRQGDAEAVVIALNMGTEPVSIASETFRLGGVILLSTFL
                     DRQGEKIHGVLDLRGNEGAIIGG"
     gene            complement(1905..3026)
                     /locus_tag="VGA15_23625"
     CDS             complement(1905..3026)
                     /locus_tag="VGA15_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX9590718.1"
                     /translation="MRPIITIRALVFALATLPLAAAFSGAVAKPTVEVAFVLDTTGSM
                     GGLIEGAKRKIWSIATAIVDSNPDAEIRMGLVAYRDIGDDYVTRTFDLTTDIQDLYAN
                     LLELRAHGGGDWPESVNEALDVAVNRLHWTPTGDTRRIVFLVGDAPPHMDYAQDTKYP
                     TTLAVARQKDIIVNAVLAGDARDTERVWRDIARNGNGRFIPIPQDGGQVVIIDTPYDD
                     DIIILQKEINGTVIPYGPRSLQKHTEDRTQQLSRAAAAAPAQASEMASYLNKRARVSK
                     EAVTGDGDLVSDVASGRQKLSTVKEDELPENLRALKPEQRMDELDRQMKQRNALNEKL
                     TALVAKRDTFVAEARDKAAPKASSFDRVVEDTLKAQIKR"
     gene            3179..3664
                     /locus_tag="VGA15_23630"
     CDS             3179..3664
                     /locus_tag="VGA15_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEX9590719.1"
                     /translation="MEKPARQVSLVPAPDRRQSETALAIARGTARLLRSLGFSCISEL
                     PLPSGRRADLVALNERSEIWIVEIKSSIEDLRADQKWQDYRMHCDRLFFAFTQDLPCE
                     IFPAETGLIVADAYGAHLHCEAPEHRLPAPTRKLMTVRFAIAAAQRINRLVDPQGHGE
                     F"
     gene            complement(3687..4214)
                     /locus_tag="VGA15_23635"
     CDS             complement(3687..4214)
                     /locus_tag="VGA15_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX9590720.1"
                     /translation="MVPRTGLHVNSRLLIDGSQMLIQKLRLQRGWSQEQLVDLSGLSV
                     RTIQRLERGQTASVESLKALGAAFEIEFSDLKEPEMDPTMSQSVRADEALAFAHVRKL
                     KGFYMNLMLYIVVIGFLVIVNLVTYPRYLWVIWTALGWGIGILFHGLRVFDMVPFLNG
                     EWERRQVEKQLGRKL"
     gene            complement(4431..5504)
                     /locus_tag="VGA15_23640"
     CDS             complement(4431..5504)
                     /locus_tag="VGA15_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX9590721.1"
                     /translation="MVAAIERISKRTDAPAQPEGSDKASDISALIARLTTEVDQIACE
                     KTKSIQQITNQMKMLALNALIESSRAGALGAGFAVVAQEVRSVGQKVEDIARELETEL
                     TGRTANLAQSIEKMMERSRGERMVDLALNAVELIDRNLYERTCDVRWWATDSAVVDCA
                     ASPGAANASHVAGRLAVILRAYTVYLDLWLCDLNGNVLASARSDRYDVKAANVARMPW
                     FSDALKLRSGDDYVAGDIERQPLLGNAQVATYCASVREGGKANGKPLGVLAIHFDWEA
                     QARAIVEGIRVDDEKARVLLVDSKFRVIASSDGQGLLTERVPLKGGRSGFYKDGSGPQ
                     VAFHVTPGYETYKGLGWFGVILR"
     gene            complement(5767..>6298)
                     /locus_tag="VGA15_23645"
     CDS             complement(5767..>6298)
                     /locus_tag="VGA15_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HEX9590722.1"
                     /translation="DHADRSLLIVEDDKAFLERLSRAMETRGFTVTSCDSVSDGLAQI
                     GKAAPAFAVVDLRLGDGNGLDVVSALKRKRPDARAIVLTGYGNIATAVTAVKMGAVDY
                     LSKPADADDVVAALLASGTEKSELPINPMSADRVRWEHIQRIYEMCNRNVSETARRLN
                     MHRRTLQRILAKRAPR"
BASE COUNT         1119 a         1943 c         1977 g         1259 t
ORIGIN      
        1 cacaatgccg attttttgcg gctcgccaag gggaaattgg gctgaaaaag tgggcggttg
       61 acgctcccgt gtcatctctt cctggttcaa actgaaccgg ctagcggttc caggcgttgc
      121 ctcgcgcggc gcaatcaaag atgatttgca gaatgaagca gagcgacgaa acctggtggc
      181 ggcacggcat cttctaccag gtctatccgc gctcgtttca ggattccaat gccgacgggg
      241 tcggcgatat cgccgggctg atcgaccggc tgccctattt gctggagctc ggaattgacg
      301 cggtctggct gtcgccgatc tttccctccc cgatggccga tttcggctac gacatttcgg
      361 actataccgg cgtcgatccg ttgttcggca ccacggccga tttcgacgcg ctcatcagcg
      421 ccgcgcatgc agccggtctc aaactcatcc tcgatctggt tccgaaccat acgtcagacc
      481 ggcatccctg gttcatcgcc agccgaagct cgcgcgatga tgggaagcgc gactggtaca
      541 tctggcgcga accggccgcg gacggcgggc cgccgaacaa ctggatgtcg gaattcggcg
      601 gcagcgcctg ggcgtatgac gccgcgacac ggcagtatta ttatcatgcc tttctcgccc
      661 agcagccgga cctgaactgg cgcaatccgg aggtacgtca ggccatccat gacgtcatgc
      721 ggttctggct ccgcaaaggt gtcgacggct ttcgggtcga cgtgatctgg catctgatca
      781 aggacgcgga gtttcgggat aatccgccca atccggattt tcgcgagggt cagcctccgc
      841 actggaaggt actgccgctg cgcaccaccg atcagcccga ggttcatgac gtgatcgcgg
      901 agatgcgccg ggtgatcgac gaattcgatg cccgggtgct gatcggcgag atctaccttc
      961 cggtcgagcg cctcgtcacc tattacggca agaatctcgc gggcgcgcat ctgccgttca
     1021 attttgcgct gttgtcggcg ccatggaacg cgcgagcaat cgagaaaatc atcgccgact
     1081 acgaagccgt actcccggcg ggggcgtggc cgaactgggt gctgggcaat cacgatcgtc
     1141 cgcgggtggc aagccgggtt gggccggcgc aggctcgcgt cgccgcgatg ctgctattga
     1201 cgttgcgggg aacgccaacg ctctattacg gcgacgagat cggcatgcac caggtggcga
     1261 ttgcgcccga tcaggtgcgc gatccctttg agaagaacgt tcccggcatc gggatggggc
     1321 gcgacggctg ccggacgccg atgcaatgga acgcaacacc ttatgccggc ttctcgacgg
     1381 tggcgccgtg gctgccgctg tcagatgatt ttgcgcagga caacgttgtc aatctcgacg
     1441 ccgacaagcg atcgaccctc agcctctaca agacgctgat cgacttgaga aagaaatcgc
     1501 cgcaactcgt gtccggcgcc tacgttccga tcgcggcaca gggcgattta ttgatttacc
     1561 gcaggcaggg cgatgcagag gcggtggtga tcgcgctgaa catggggacg gagccggttt
     1621 cgatcgcgtc ggagacgttt cgccttggcg gcgtaattct gctttcgacc ttcctcgatc
     1681 ggcaaggcga aaaaatccac ggcgtgctgg atctgcgcgg caatgagggt gccatcatcg
     1741 gcggataaag ggacaagagc taacgtcgtc ccggcgaagg ccgggaccca tcacccctgg
     1801 cgttcgagtt aaggcaggca ttggctacct tctctaatca gcaacgacac ggagtatggg
     1861 tcccggcgtt cgccgggacg acgatcgtgt taatcttcgt gaagctacct tttaatctgc
     1921 gccttcagcg tatcctcgac cacgcgatcg aaggacgacg cctttggcgc ggccttgtcg
     1981 cgcgcttcgg cgacgaatgt gtcgcgcttt gcgaccaggg cggtgagctt ttcgttcaat
     2041 gcgttgcgtt gcttcatctg cctgtcgagt tcgtccatgc gctgctcggg tttcagcgcg
     2101 cgcagatttt ccggcaattc gtcttccttc accgttgaaa gcttctgacg tccggacgcg
     2161 acgtcgctga ccagatcacc atcgccggtg actgcttcct ttgacaccct ggcgcgtttg
     2221 ttgagatagc tggccatttc gctggcctgc gccggcgccg cggcggccgc ccgcgacaat
     2281 tgctgggtcc ggtcttcggt gtgcttttgc agagaccgcg ggccataggg gatcacggtg
     2341 ccgttgatct ctttctgcag gatgatgatg tcgtcatcat agggcgtgtc gatgatgacg
     2401 acctgtccgc cgtcctgcgg gatcggaatg aagcggccgt tgccgttccg ggcgatatcg
     2461 cgccacaccc gttcggtatc gcgcgcgtcg ccggccagca ccgcgttgac gatgatatcc
     2521 ttctgtctcg cgaccgccag cgtggtcgga tatttggtgt cctgcgcgta gtccatgtgc
     2581 ggcggcgcat cgccaaccag gaacacgatc cgcctggtgt cgccggttgg cgtccagtgc
     2641 agcctgttga cggcgacgtc gagcgcctcg ttgacgcttt ccggccagtc gccgccgccg
     2701 tgcgccctca actccaagag gttggcgtag agatcctgga tgtcggtggt gagatcgaag
     2761 gtccgggtga cgtagtcgtc gccgatgtcg cgataggcga cgaggcccat gcgaatctca
     2821 gcgtcggggt tggagtcgac gatcgcggtc gcgatcgacc agatcttgcg cttggcgcct
     2881 tcgatcaggc cgcccatcga gcctgtggtg tccagaacga aggcgacttc aacggttggc
     2941 ttggcgacgg ctcctgaaaa cgccgccgct agtggcagtg tggcgagtgc gaatacgagt
     3001 gcgcggatgg tgatgatggg tctcatggtt ttcctccaat ggtccgtccc ctggaaccag
     3061 gttcccctcg cttcacggtc gcgtcgggtt ggaagcaaga cgggtgcgga caaatttacg
     3121 gccgacttgg cttgggccag cctgttggac ggcctgaaat cggataaggt tcacggtcat
     3181 ggaaaagccc gcccgccagg tcagtctcgt gcccgcgccc gaccgtcgtc agtcggagac
     3241 ggcgctggcg attgcgcgcg gcaccgcgcg gctattgcgg tcgctggggt tttcctgcat
     3301 cagcgaattg ccactgccct cgggacggcg tgccgatctg gtggcgctga acgagcgcag
     3361 cgagatctgg atcgtcgaaa tcaaatcctc gatcgaggat ctgcgcgccg atcagaaatg
     3421 gcaggactac cggatgcatt gcgatcggct gttctttgcc ttcacccagg atctgccctg
     3481 cgagattttc cctgctgaaa ccggcctgat cgtcgccgac gcctatggcg cgcacctgca
     3541 ttgcgaggca ccggagcatc ggctgccggc gccgacccgc aaactgatga cggtgcgttt
     3601 tgccattgca gcggcacagc ggatcaaccg cctggtcgat ccgcagggcc acggggaatt
     3661 ttagaccgaa tattcataga atgacgtcag agcttgcgtc caagctgctt ctcgacctgc
     3721 cgccgctccc attcgccgtt caggaaggga accatgtcga acacgcgaag gccgtgaaac
     3781 aggatgccga tcccccaacc cagcgcggtc cagatgaccc agagatagcg gggatatgtc
     3841 acgaggttca caatcacgag gaagccgatg acgacgatgt acagcatcag gttcatatag
     3901 aaccccttca gtttgcggac atgtgcaaac gcgagggctt cgtcggcgcg gacgctttgg
     3961 ctcatggtgg ggtccatttc cggctccttc aaatcagaga attcgatctc aaaggccgcc
     4021 cccagcgctt tcagggattc gacgctggcg gtttgtcccc gttcgaggcg ttgaatcgtc
     4081 cgaacgctca gtccgctgag atcgaccagt tgctcttgcg accagccccg ttgcagcctg
     4141 agcttctgaa tcaacatttg agacccgtca atcaacaaac gtgaatttac gtgaagaccg
     4201 gtccgcggca ccacgtcagc gagccgccag ctgcccgcca gcgccccgac atgaacacga
     4261 cagcaggaca aaataccccg gggcaagcct ggatggctcg aaaatgcccg ggattttgct
     4321 aggcggccgc ttatgctcca acccgtcaaa tcacgatcgg ccggccgtgc acccggattg
     4381 ccgtgctcga ggatttgcgc tgacgtatgg cgcgctgcgc ccgccttgcc ctaacggagg
     4441 atcacgccga accatccgag gcctttgtag gtctcgtagc cgggggtgac atgaaacgcc
     4501 acctgcgggc cggagccgtc cttgtagaat ccgctgcgtc cgcccttcag gggcacccgc
     4561 tcggtgagca ggccctggcc gtcggaggac gcgataacgc ggaatttcga atccaccagc
     4621 agcacgcggg ccttttcgtc atccacccgg atgccttcga cgatggcgcg ggcctgcgct
     4681 tcccagtcga aatggatcgc gagaacgccg agcggcttgc cattggcctt gccgccttcg
     4741 cgcacgctgg cgcaataggt cgcgacctgg gcgttgccga gcagcggctg acgctcgatg
     4801 tcgccggcga cataatcgtc gccggatcgc agcttgagcg catcggaaaa ccatggcatt
     4861 cgggcgacgt tggccgcttt gacgtcgtag cggtccgagc gggcgctggc cagcacattg
     4921 ccgttgaggt cgcacagcca cagatcgaga tagacggtat aggcgcgcag gatcaccgca
     4981 aggcgcccgg cgacgtggga agcattcgcg gcgcctggac tggcggcgca atccacgacg
     5041 gcggaatcgg tggcccacca gcgtacgtcg caggtgcgct cataaaggtt tcgatcgatc
     5101 agctcgacgg cgttgagcgc gagatcgacc atccgttccc cgcgtgagcg ctccatcatc
     5161 ttttcgatcg actgcgccag gttggcggtc cgtccggtca gctcggtttc caactcgcgg
     5221 gcgatgtcct ccaccttctg cccgacgctg cggacttcct gcgccaccac ggcaaagccc
     5281 gcgccgagcg cgccggcgcg cgagctttcg atcagcgcgt tcagcgccag catcttcatc
     5341 tggttggtga tctgctggat cgatttggtc ttctcgcagg cgatctggtc gacctcggtg
     5401 gtcaatcgcg cgatcagggc ggaaatatct gacgctttgt cgcttccctc gggttgcgcc
     5461 ggcgcgtccg tccgtttgct gatgcgctcg atcgcagcga ccatcggcct tgtccctttg
     5521 cggctggttc cggcctgtcc agaccacagg cgccgggcgc gccggtgaca gcaacagact
     5581 gtctgtttat cgctggctat gcctaacgag cccttaattc ccggtgcgtt caggccgcaa
     5641 aaataccgcc tgccgtgatg aaagaaacgg caggttcaat tctgcccgcc aatcgcgcat
     5701 gggctcgcga cggctgccga ttggccggtc gattcgcaag gcggcaggcc cagatatcga
     5761 gaaaaattac cgcggcgcgc gcttggcgag gatgcgctgc agggtgcggc gatgcatgtt
     5821 gagccgacgt gcggtttcag agacgttgcg gttgcacatc tcgtagatgc gctggatgtg
     5881 ctcccagcgg acgcggtccg ccgacatggg attgatcggc agctcggact tctcggtgcc
     5941 gctcgccagc aatgccgcga cgacgtcgtc ggcgtcggcg ggttttgaca gataatccac
     6001 cgcgcccatc ttcaccgccg tcacggcggt ggcgatgttg ccataacccg tcagcacgat
     6061 cgcgcgcgcg tcggggcgct tgcgcttcaa cgccgacacc acgtcaaggc cattgccgtc
     6121 gccgagccgc agatccacca ccgcgaaggc tggtgccgcc ttgccgattt gcgccaaacc
     6181 atcggatacg ctgtcgcagg atgtgaccgt gaagccacgg gtctccatgg cgcgcgacag
     6241 ccgctccaga aacgccttgt cgtcctccac gatcaggagc gagcgatcgg cgtgatca
//
LOCUS       DASWGM010000869         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1063573,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000869 DASWGM010000000
VERSION     DASWGM010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063573"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            556..858
                     /locus_tag="VGA15_23650"
     CDS             556..858
                     /locus_tag="VGA15_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX9590723.1"
                     /translation="MTLDAARQPGSKRIPTLDIGDNGEATLDLWCKGGKGQFSVAGNT
                     VIFVAGPLEDRACPPARAQADDELVAALSEAATWKRQGDLVSFIGTKPLRFRINTN"
     gene            complement(954..2546)
                     /locus_tag="VGA15_23655"
     CDS             complement(954..2546)
                     /locus_tag="VGA15_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9590724.1"
                     /translation="MARYDVVVIGAGLGGLTAGAILARAGRKVLVIERNNSVGGAASS
                     YKSGDLFVEGSLHETSDPHHPRDPKHNVLTRAGVIDAVKWIPAGAFYEARGGPLGQPF
                     VLPDNFDAARRALTERFPEARAGIDQLLEEMERIASAIGTLSQGSGAFRKPREALGAL
                     LNLLSGFPDWRLSLSQKLDRVFGDNEAVKCALAANLSYYHDDPATLWWVFFAIAQGGY
                     LLSGGRYVQGGSQRLSSALARAIKVAGGEVLVRRVVSGIAPGLQGHSSTVTHSARDGS
                     DAQAVECVRIVGNAAPAALAPLMPAAAAEKLAANDRRHMPSISLFALTLGLSRPPREF
                     GISAYSTQLLPPEMARLSDYAQGTALMAEEPEKRMPPMSIVDYAAIDSGVPAPPYVLL
                     ICGPDRLSNWDSSDMDAYREKRGRWQQAIVGYLSSVYPGLADAVVASSFNTALSVRQY
                     LNAPDGAVYGFAPTPPRSIWRVPVRSPRTAVPGLYLASAYAGFGGYTGVVQSAGACAD
                     MILRESWSPVSIESKPRSRFLI"
     gene            complement(2728..>3594)
                     /locus_tag="VGA15_23660"
     CDS             complement(2728..>3594)
                     /locus_tag="VGA15_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590725.1"
                     /translation="KLTRRPTGDTFEFRGQISVGQTVTEVTSTDNSDLSEKEFQDNQA
                     TDDGIAAAPKDFTEVSPEAVGVKVKLEAALDFLKGLKGQDVEVSLSSLTVSCEALRAG
                     EQTINISVDPDHAAALIAKSKSLPGVVAAGWTSGVVEIDRSIRFAAADWRDGDKLNKD
                     KLAAAVANVLSKTLSAKSAAATWSASTGKLKLTFKRPSQIFPALGLTETIEILALVAP
                     DKPGTTDRLMLWIGNPVISTTDETAGAKLNLAEEASADEEGDAKDDNGSVDALAKEFK
                     AQRWDADKSAWK"
BASE COUNT          655 a         1132 c         1125 g          682 t
ORIGIN      
        1 caccgcgacc aactggtacg gacacgaaag cacgatcctg cgcttcgtgt tcaagcattc
       61 gatcgcgctg gcgtgtctcg tcggcctgct cgtcatgctg caggcctatg tctatccttt
      121 tacggagatg gtcctgaagt aagacgccgg gcttgacacc cagacccgca cagatccccg
      181 cggcccgcgc cgcggggatt tgctgagccg atgatttttg tggctgccag tccggacaag
      241 gtcgcgcttg ccgatcgcgc gcgatcggca aaaacgaatc cggcttttta tgggacaaat
      301 gtcttcgttg gtcctgagat cgcgctttaa atttttttgt aagtgcgcgc gatctttttc
      361 gggaaaccgc tcacactttg ccggatcatg cgctagaacg acgcgcagct gtaggtttcg
      421 aatagttcga ggagttgtat gccgttagcg acaagatttt tggggcaggc cgccgcagtg
      481 ctcgtgctgg ccacggccac cttcaaagcc atcccggcgg tctgcgccga agacgagttt
      541 ccgtttgggt tggagatgac cctggatgcg gcgcgtcagc ccggatcgaa gcggataccg
      601 acgctggaca tcggcgacaa tggcgaggcg acgctcgatc tgtggtgcaa gggcggcaag
      661 ggtcaatttt cagttgcggg caataccgtg atttttgtcg caggacccct ggaggatcgc
      721 gcctgcccgc cggcgcgggc gcaggctgac gatgaactgg tcgcagcatt gagcgaggcc
      781 gctacctgga agcggcaggg cgatctcgtt tcattcatcg gaaccaaacc gctgcgcttc
      841 cgtatcaata ccaattgaat gactggccaa agcattttca agcgaagcat gccccaggac
      901 ttgattcggg acctgatccg ggggtggaga ccggttcgcg tcaagaaaac gcgtcaaatc
      961 aagaatctgg agcgcggttt cgattcgatc gaaacggggc tccagctctc gcgcaagatc
     1021 atgtcggcgc aagcgccggc ggattgcaca acgccggtgt agccgccaaa ccccgcatag
     1081 gcggacgcca gatagaggcc cggcaccgcg gtgcgcggcg agcggaccgg gacgcgccag
     1141 atcgagcgtg gcggcgtcgg cgcgaaccca tagacggcgc cgtcaggcgc attgagatat
     1201 tgccgcaccg acagcgcggt attgaacgac gacgccacca cggcgtcggc gaggcccgga
     1261 taaaccgaag agagatagcc gacgatcgcc tgctgccatc gcccgcgctt ctcgcgatag
     1321 gcgtccatgt ccgaactgtc ccaattcgac agccggtcgg gcccacagat caacagcaca
     1381 tagggcggag cggggacgcc ggaatcgatc gcggcgtaat cgacgatcga catcggcggc
     1441 attcgtttct cgggttcctc cgccatcagc gcggtgcctt gcgcgtagtc ggacagccgc
     1501 gccatttcgg gcggcagcag ttgcgtcgaa taggccgaga tcccgaactc gcgcggcggc
     1561 ctcgataggc cgagcgtgag cgcgaacagc gaaatcgatg gcatgtgccg ccggtcgttt
     1621 gcggccaatt tttccgcggc cgcggcgggc atcaatggcg ccagcgcggc aggcgcggca
     1681 ttgccgacga tccgtacgca ctccaccgcc tgggcatcgc tgccatcgcg cgcgctgtgc
     1741 gtgaccgtgc tcgaatggcc ctgcaaaccc ggggcgatgc cgctgacgac gcggcgcacc
     1801 agcacctctc cgccggccac cttgatggcg cgggcaagcg cgctggagag ccgttgcgaa
     1861 ccgccttgca cgtaacggcc gccgctcaag agatagcctc cctgcgcgat cgcgaagaac
     1921 acccaccaca gggtcgcggg gtcgtcgtga taataggaaa gattggcggc caacgcgcat
     1981 ttgacagctt cgttgtcgcc gaagacccgg tccagctttt gcgacagcga caggcgccag
     2041 tcggggaacc cagataacaa gtttaacagg gcgcccagag cttcgcgcgg ctttctgaag
     2101 gcgccgctgc cctgcgaaag cgtgccgatc gcggatgcga tgcgctccat ttcttccagc
     2161 agttgatcaa tcccggcgcg ggcctccggg aagcgctcgg tcagggcgcg ccgcgccgcg
     2221 tcgaaattgt ccggcagcac aaacggctgg ccgagcgggc cgccgcgggc ttcgtaaaac
     2281 gcgccggccg ggatccattt taccgcgtcg ataacgcccg cacgcgtgag cacattgtgt
     2341 ttcggatcgc gaggatggtg cggatcgctg gtttcgtgca acgagccttc gacgaacaga
     2401 tcgccggatt tgtagctcga cgccgcgccg ccgaccgagt tgttgcgctc gatgacgagg
     2461 accttgcgtc ccgcgcgcgc caggatcgcg ccggcggtca agcctccaag gccggcgcca
     2521 atcaccacta cgtcatagcg cgccattcag cgtgaaatcc cgaaagccct tgcccagata
     2581 cgccttgttc attgccgcaa tggtgcggcg tgtcaaatgc gtccatgcat ggagcgggtg
     2641 cgtgcggggc tgcccttcga attgacagcg cttattccgc aaacgcggac accggttttg
     2701 cgatcacgca aattattgga acctccgtta tttccaggcc gatttatccg catcccagcg
     2761 ctgggccttg aattccttcg ccagcgcatc gaccgagcca ttgtcgtcct tcgcatcgcc
     2821 ttcctcgtcg gcgctggcct cctcggccag attgagtttc gcgccggcgg tttcgtccgt
     2881 cgtcgaaatg acgggattgc cgatccacag catcagccgg tcggtcgtgc cgggcttgtc
     2941 cggcgccacc aaagcgagaa tctcaatggt ctcggtgagg ccgagggcgg ggaatatctg
     3001 gctcggccgt ttgaacgtca acttcagctt gccggtactg gcgctccatg tcgcagccgc
     3061 cgacttcgcc gagagcgttt ttgacaggac attggcgacc gccgcagcga gcttgtcctt
     3121 gttgagcttg tcgccgtcgc gccagtcggc ggccgcaaaa cggatcgagc gatcgatctc
     3181 cacgacaccg ctggtccagc cggcggcgac cacgccgggc aacgatttcg acttcgcgat
     3241 cagcgccgcg gcgtgatccg gatcgacact gatgttgatc gtctgctcgc cggcgcgcag
     3301 cgcctcgcat gagacggtga ggctgctcaa ggaaacctcc acgtcctggc ccttcaatcc
     3361 cttcaggaaa tccagcgccg cctcgagctt taccttcacg ccaaccgctt ccggggaaac
     3421 ttcggtaaaa tccttgggcg ctgccgcgat gccgtcatcg gttgcctggt tgtcctggaa
     3481 ttccttttca ctgagatcgg aattgtccgt cgaggtcacc tccgtcacgg tctggccgac
     3541 gctgatctgg cccctgaatt cgaaggtgtc gccggtcggc cggcgcgtca gctt
//
LOCUS       DASWGM010000870         5250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1467270,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000870 DASWGM010000000
VERSION     DASWGM010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467270"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..131
                     /locus_tag="VGA15_23665"
     CDS             3..131
                     /locus_tag="VGA15_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX9590726.1"
                     /translation="MALLGGFSGIGGGPFYGTGYYGGGGLGLIVVILLILLLLGRL"
     gene            complement(286..2958)
                     /gene="ligD"
                     /locus_tag="VGA15_23670"
     CDS             complement(286..2958)
                     /gene="ligD"
                     /locus_tag="VGA15_23670"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX9590727.1"
                     /translation="MSLRNLSTYRKKRDFEKTAEPSGEAAIAPSKQRRFVIQKHDATR
                     LHYDLRLEFDGVFKSWAVARGPSLDPHDKRLAVEVEDHPLDYGDFEGTIPKGQYGGGT
                     VQLWDRGYWESDDPERGFKKGDLKFTLHGDKLGGSWVLVRMRNDRNGGKRTNWLLIKH
                     RDDFAREGEANNVLDADQSVASGRTMQQIAEGKGKAPKPFMTLKSGRGKADAVWNSNR
                     GEAAEARSENRAATSRSAPAAKQPSKGKKVSALPDFVAPELCISVDRPPAAEGWCHEI
                     KFDGYRVQLRVEGGEATLKTRKGLDWTEKFQAIAKEGSALPDVLIDGEIVALDHNGAP
                     DFSTLQAALSDGKTDVLIFYAFDLLFADGEDLRAVPLGERKARLKKLLEARAKNKGQL
                     IRYVEHFETGGDAILQSACKLSLEGIVSKKLDAPYHSGRSENWTKAKCRAGHEVVLGG
                     WKTTNGKFRSLMAGVYRGDHLAFVGIVGTGFGQDTVRRIMPALKAAASDKSPFGGKDA
                     PKKTRDVHWLKPELVAEIEFAGWTDGGNIRQAAFKGLRQDKPADEVKAEKPAMTRIAK
                     PTGKIAAKAGKAGTSNRTQLRSADSTVMGVAISKPDKKLWPDGGDGEGVTKLDLARYF
                     EAVGGWMIGHLKGRPCSVVRAPDGINGERFFQRHAMPGTSNLLELVKVSGDRKPYLQI
                     DRVEGLAAVAQIGGLELHPWNCAPDAPDAPGRLVFDLDPAPEVEFSDVIEAAKDMRQH
                     LTDVGLESFCKTTGGKGLHVVTPLAYGAKDKVTWKEAKAFAQGVCQWMADEDPERYLL
                     NMSKKLRKGKIFLDYLRNDRMATAVAVLSPRAREGATVSMPLTWAQVRGDLDPKRYTV
                     RTVPALLAKTKAWEGYDDAASSIQAAIKKLSSRK"
     gene            3143..3367
                     /locus_tag="VGA15_23675"
                     /pseudo
     CDS             3143..3367
                     /locus_tag="VGA15_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
     gene            complement(3487..3699)
                     /locus_tag="VGA15_23680"
                     /pseudo
     CDS             complement(3487..3699)
                     /locus_tag="VGA15_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
     gene            3948..4964
                     /locus_tag="VGA15_23685"
     CDS             3948..4964
                     /locus_tag="VGA15_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012753786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEX9590728.1"
                     /translation="MNVASSAVLATEPVNSEGVVAAGAYVDGRRVANIAIDEASSWRS
                     RPGHVVWIGLHEPDMALLTGVQRQFQLHDLAIEDADHAHQRPKIEQYGDALFIVARTA
                     QLVGDSIAFGETHLFVGEGYLVSVRHGASTSYTPVRERCESCPRALARGEDYILYAIL
                     DFIVDNYSPVLETIQEEVEAMEALVLASAMTRMQIERLYLLRRDLLRLRNAVGPLVEV
                     CRRLEHDNLPMVRPTMQPLFRDVTDHVRTVQEQIDSLREVLAFAFEASLLVGQAQETA
                     VSKKLASWLAIIAVPTAIAGIYGMNFKNMPELQWEYGYVTVMGVILAACAALYWRFRR
                     VGWL"
     gene            complement(4974..>5250)
                     /locus_tag="VGA15_23690"
     CDS             complement(4974..>5250)
                     /locus_tag="VGA15_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX9590729.1"
                     /translation="TGIGRGVAIPHARLPDLQRPYGLLAKLKQPIEFDAIDGQPVDLV
                     FTLLLPAAAENGQLEPLALVARKLRAPENLVRLRGAKNTSELYSAMV"
BASE COUNT          949 a         1669 c         1536 g         1096 t
ORIGIN      
        1 ttatcgcgct gcttggcggc ttcagcggca tcggcggcgg accgttctac ggcaccggct
       61 attatggcgg cggcggcctt ggtctcatcg tcgtcatcct gctgattctg ctgctgctcg
      121 gacgactcta ggttttcgta gccaatggcg cgggattgag tgatgctcaa tctcgctcat
      181 ttttttgtgc atcgcatgac gtgacgtact gcgcttcctc accataggct gtcattatcc
      241 gcgtacgcgg agtatgacga cacagagacc cttccacagt ggtaatcatt tacgcgagct
      301 cagcttcttg atcgcagcct ggatcgatga cgccgcatcg tcatagcctt cccacgcctt
      361 ggtcttggca agcagcgccg gcacggtgcg aaccgtgtag cgtttcgggt cgagatcgcc
      421 gcgcacttgc gcccatgtca gcggcatgga gacggtggcg ccttcgcggg cccgcggcga
      481 cagcacggcg acggcggtgg ccatgcggtc gttgcgaaga taatcgagga agatctttcc
      541 cttgcgcagc ttcttcgaca tgttgagcag atagcgctcg ggatcctcat cggccatcca
      601 ctggcaaacg ccctgggcga acgccttggc ctctttccac gtcaccttgt ctttggcgcc
      661 gtacgccaac ggcgtcacca cgtgcagccc cttgccgccg gtggtcttgc aaaaactctc
      721 caggccaacg tccgtcagat gttgccgcat atccttggcg gcctcgatga cgtcggaaaa
      781 ttcgacctcc ggcgcagggt cgagatcgaa taccagccgt cccggcgcgt caggcgcatc
      841 cggcgcgcaa ttccacggat gcagttcgag gccgccgatc tgcgccaccg ccgcgagacc
      901 ctcgacgcgg tcgatctgca gataaggctt gcggtcgccg gagactttta caagctccag
      961 gaggttagag gtgccgggca tggcgtggcg ctgaaagaat ctctcgccgt ttatgccgtc
     1021 gggggcgcgg accacggaac atggccgtcc cttcaggtga ccgatcatcc agccgccgac
     1081 cgcctcgaaa tagcgggcga gatcgagttt tgtaactccc tcgccgtcgc cgccgtccgg
     1141 ccacagcttc ttgtcgggct ttgagatagc gacgcccatc accgtgctat ccgccgacct
     1201 cagctgcgtc ctgttcgacg tgcccgcctt cccggctttc gcggcgatct tcccggtggg
     1261 tttggcgatc ctggtcatgg ccggcttttc cgccttcacc tcgtcggccg gcttgtcctg
     1321 gcgcagcccc ttgaacgcag cctgccgtat attgccgccg tcggtccagc cggcaaattc
     1381 gatctcggcg acgagctcgg gcttcagcca atgcacgtcg cgggtcttct tcggtgcatc
     1441 cttgccgccg aacgggctct tgtcggacgc cgcggccttc aacgcgggca tgatgcgcct
     1501 gaccgtgtcc tgcccgaagc cggtgccgac gataccgacg aatgcaagat gatcgccgcg
     1561 ataaaccccc gccatcagcg agcggaattt gccgttggtg gttttccacc ctcccagcac
     1621 gacctcgtgc ccggcgcggc atttggcctt ggtccagttc tcggaccggc cggaatgata
     1681 gggcgcgtcg agcttcttcg agacgatgcc ttccagggac agcttgcagg ccgattgcag
     1741 gatggcatcg ccgccggttt cgaaatgctc gacataacgg atcagttggc ctttgttctt
     1801 cgcccgcgcc tccagcaatt tcttcaatcg cgccttacgt tctccgagcg gaaccgcgcg
     1861 aagatcttcc ccgtcggcaa acaacaggtc gaacgcgtag aagatgagaa catcggtctt
     1921 gccgtcggaa agcgcggcct gcagcgtcga aaaatccggc gcgccattgt ggtcgagtgc
     1981 gacgatctcg ccgtcgatca gcacatccgg aagcgcgctt ccctctttgg cgatggcctg
     2041 aaatttttcg gtccagtcca ggcctttccg ggtcttcagt gtggcctcgc caccctcgac
     2101 acgcaattgc acccggtagc cgtcgaattt gatctcgtgg caccagcctt ccgcggcggg
     2161 cggacgatcg acggaaatgc agagttctgg cgcgacgaaa tccggtaatg cgctgacctt
     2221 cttgcctttg gacggctgct tggcagccgg cgcggatcgc gatgtagctg ccctgttctc
     2281 agaccgggct tcggcggctt cgccccggtt cgaattccag accgcgtcgg ccttcccgcg
     2341 cccggacttc aacgtcatga acggctttgg cgccttgccc ttgccctcgg caatctgctg
     2401 catcgtgcgc cccgaggcga ccgattgatc ggcatccaaa acgttgttgg cttcgccttc
     2461 cctggcaaaa tcgtcgcgat gcttgatcaa cagccaattg gtgcgcttgc cgccgttgcg
     2521 gtcgttgcgc atccgcacca acacccagct gccgcctagc ttgtcaccat gcagcgtgaa
     2581 tttcagatcg cccttcttga atccccgttc cggatcgtcg gattcccagt agccgcgatc
     2641 ccagagctgc acggtgccgc caccatactg tcccttcgga atggtgcctt cgaaatcgcc
     2701 gtagtccagc ggatgatctt ccacctccac cgcgagccgc ttgtcgtgcg gatcgagcga
     2761 ggggccgcgc gcgaccgccc aggatttgaa gacgccgtcg aactccagcc gcagatcata
     2821 atgcagccgg gtcgcatcat gcttctggat cacgaaccgc cgctgcttcg aaggtgctat
     2881 cgccgcttcg ccgctgggct ccgcggtctt ttcaaaatcg cgctttttcc gataggtgga
     2941 gaggtttctc aaggacacga tcaaatccca cgccaatgaa taagcggcac cctatggcac
     3001 gacagcaagg gcccggaacc aaaacccaga atatcagttg aagttcccga attccctttg
     3061 acctataaca agccggagat tgtaatggcc gttcgcgcct attggaaagg ctccttaaag
     3121 ctttccctcg tgtcatgccc gtgccttgaa gacactggtc aggcgcaagg ccgccggcaa
     3181 gccggtcaaa tcagccgtgc gcgaggaaaa gccggacaat gtcatcagcc tgatggatgc
     3241 gctgcggcaa agcctgaagg gcaaagcatc ggcgaagcgg tcggcgcatt cccgggcgcg
     3301 ccgggcgatg caacgccccg caaaaaaagc gcaccgctcg gcggcgcgac cgcgcaaggc
     3361 gagttaacct ccgtcatgcc cgggcttgtc ccgggcatcc acgtcttaac agcatcgcaa
     3421 tgaaaaagac gtggatggcc gggtcaagcc cggccatgac gagaaatcta tgacggccgg
     3481 ctatctcttc gccttacgct tcttggccgt cttcttcttg gaagccttct tcgggacttt
     3541 cgctcccgca gcccgcgctt cggaaagacc gatcgcgatc gcctgcttgc ggctcttgac
     3601 cttcttgcct gatcgaccgc tcttcaaagt gccggccttg cgcttcttca tcgcgcgccc
     3661 gactttggcg gacgcctttt tcgaatattt tcgtgccatg attattctcc tgagttgcga
     3721 agagaataat cttcgccctc gaagctggtt cccgcgagga agaaggcagg aatcggagcg
     3781 ggtgtgagtt gacctttgta tgattgaagg cgtggccgaa ccaggaccgc cgatccatca
     3841 ccgacgaagt cccgacattc catttgacgg cgggttttcc agtgctagcc ttcccgaaag
     3901 gctttgcctc ggagcaaagt tcaacagcgg gcattgggac ggaaaccatg aacgtagcgt
     3961 catcagccgt cttggcgacg gagcctgtca actccgaagg tgtcgtcgca gccggcgcct
     4021 atgtcgatgg ccggcgcgtc gccaatatcg ccatagacga agcttcaagc tggcgcagcc
     4081 ggccaggcca tgtggtgtgg atcggactgc acgaaccgga tatggcgctc ttaaccggcg
     4141 tgcagcggca attccagctg cacgatctcg ccatcgagga tgcggaccac gcccaccagc
     4201 gaccgaagat cgaacaatat ggcgacgcgc tgttcatcgt ggcgcgcacc gcgcaactcg
     4261 tgggcgacag catcgcattt ggcgagaccc atctgtttgt cggcgaagga tatcttgttt
     4321 ccgtccgtca tggtgcgtcg acgtcttata cgccggtacg cgaacgctgc gaaagctgtc
     4381 cgcgagcgct ggcgcgcggc gaggactata tcctctacgc catcctcgat ttcatcgtcg
     4441 ataactactc gccggtgctc gaaaccatcc aggaagaagt cgaggccatg gaggcgctcg
     4501 tgcttgcgag cgcgatgacg cggatgcaaa tcgagcggct ttatctgctg cgacgcgacc
     4561 tgttgcgcct gcgaaacgcg gtcgggcccc tggtggaggt ctgccgcaga cttgagcatg
     4621 acaatctgcc gatggtgcgg cctaccatgc agccgctgtt ccgcgacgtc acagaccacg
     4681 tcaggacggt tcaggagcag atcgactcgc ttcgagaggt cctcgcattt gccttcgagg
     4741 cgagtcttct ggtcggtcag gctcaggaga ccgcggtctc caagaaactg gcctcatggc
     4801 tggccatcat cgcggtcccg accgcgatcg ccggaatcta cggcatgaac ttcaagaaca
     4861 tgccggaact gcaatgggaa tatggttacg tcaccgttat gggcgtgatc ctggctgcgt
     4921 gcgcggcgct gtactggcga ttccggcgcg tcggatggct gtgagcccgg ccgtcagacc
     4981 atcgccgaat aaagctcgga cgtattcttt gcgccacgca atcgaacgag attctccggc
     5041 gctcttaatt tccgggccac cagcgcgagc ggctcgagtt gtccgttctc cgcagccgcc
     5101 ggcaacaaaa gtgtaaatac gagatccacc ggttgcccgt cgatggcgtc gaattcgatc
     5161 ggctgcttca gtttcgcaag cagcccgtag ggccgttgca aatcgggcag tcgcgcatgc
     5221 ggaatggcta cgccacggcc gatccccgtc
//
LOCUS       DASWGM010000871         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_782740,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000871 DASWGM010000000
VERSION     DASWGM010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782740"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VGA15_23695"
     CDS             complement(<1..996)
                     /locus_tag="VGA15_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX9590730.1"
                     /translation="MDDLLREFLTETSESLDTVDNQLVRFEQDPNNAKILDNIFRLVH
                     TIKGTCGFLGLPRLEALAHAGETLMGKFRDGMPVTAEAVTLILGSIDRIKEILGGLEA
                     TETEPEGTDQDLIVKLQAMVERGIQAMSASAMPIASASAAPVASAPAVKPIEQGTLVP
                     QILERELRPGEVSLDELERAFRETATEIAPPLAPAPRPAPAPKQDAASPKEAKPVVKK
                     ANPDPEAAEGNSIANQSIRVNVDTLEHLMTMVSELVLTRNQLLEISRRNEDTEFKVPL
                     QRLSNVTAELQEGVMKTRMQPIGNAWQKLPRIVRDLAGELHKQIELEMHGADTELD"
     gene            complement(1188..1835)
                     /locus_tag="VGA15_23700"
     CDS             complement(1188..1835)
                     /locus_tag="VGA15_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEX9590731.1"
                     /translation="MSGTSSPVPAPDALELAALLCSRVCHDLISPVGAIVNGLEVLDD
                     NPKPDDREFALDLIRKSAKTASARLQFCRLAFGAAGSTGAQIDLGDAQTMARGHLEDS
                     KTTIAWNLPRVLLAKNRVKLLLNMMVIAQQTIPRGGVLTVDPVGEGDAIGFRVSAAGL
                     NARMPQNIADLLSADSTSSVDAHAVQPYYTRLLAEACGLKVVLAPEGEAVVVTAS"
     gene            1972..2805
                     /locus_tag="VGA15_23705"
     CDS             1972..2805
                     /locus_tag="VGA15_23705"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX9590732.1"
                     /translation="MNEAHSSRPAIDCDAAAALMDPLTDLVIRAGAAIVAINRVAMKI
                     DGKADGSPVTEADLAADRIIGEGLARLFPEIPALSEERVDPAKPPYRGSFFLIDPLDG
                     TKEFVAGRDEFTVNLALVTNATPLLGIIGAPALGLIWRGLVGCGAQRLTIAEGSVRDV
                     EPIHTRPFPRGGAPWIAAVSRSHGDTRTEAFIEARPGAIRQALGSAVKFGRVAEGGAD
                     IYPRLAPTSEWDIAAGHAIVTAAGGKITDANGKDLRFGERRDGFIVPEFIAWGDPAAV
                     R"
     gene            complement(2810..>3055)
                     /locus_tag="VGA15_23710"
     CDS             complement(2810..>3055)
                     /locus_tag="VGA15_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590733.1"
                     /translation="GNRASFVAHPDIYGPQFGGYDPVDLARGVTYAGNPRFWLIAGQR
                     LYLFGREDNRDAFAADPERFQRKATSRWPALEQQLAQ"
BASE COUNT          571 a          960 c          943 g          581 t
ORIGIN      
        1 gtcgagctcg gtgtcggcgc cgtgcatctc cagttcgatc tgcttgtgaa gttcgccggc
       61 cagatcgcgc acgattcgcg gcagcttctg ccaggcattg ccgatcggct gcatccgcgt
      121 cttcatgacg ccttcctgca gctcggcggt gacgttcgac agccgctgca gcggcacctt
      181 gaactcggta tcctcgttgc gcctggagat ctccaagagc tgattgcggg tcagaaccag
      241 ttccgacacc atcgtcatca gatgttccag cgtatccaca ttgacccgga tcgactggtt
      301 ggcgattgag ttgccctcgg ctgcttccgg atctggattg gcttttttca cgaccggctt
      361 tgcctctttc ggtgacgcag cgtcctgctt gggcgcgggt gcgggtcgtg gcgccggcgc
      421 cagcggcggc gcgatctcgg tcgcggtctc gcggaacgcc cgctccagtt catccagcga
      481 cacttcgcca ggacgcaact cacgttccag tatctgcggc accaatgtgc cttgctcgat
      541 gggcttgacg gcaggcgctg acgcgaccgg cgcagccgac gctgacgcga tcggcatggc
      601 cgacgctgac atcgcctgga tgccgcgctc gaccatcgcc tgaagcttga cgatcagatc
      661 ctgatcggtg ccttccggct cggtttcggt cgcctcaagc ccgccgagaa tttccttgat
      721 gcggtcgatg ctgcccagga tcagcgtgac cgcctcggcg gtaaccggca tcccgtcacg
      781 gaatttgccc atcagggtct cgccggcatg ggcgagcgct tccaggcgcg gcagcccgag
      841 aaagccgcag gtccccttga tggtgtggac caggcgaaaa atgttatcca ggatcttggc
      901 gttattcgga tcctgctcga accgcaccag ctgattatcg accgtatcca gactctcgct
      961 ggtttccgtc aggaactccc gcaacaaatc atccatgaaa ctagccttca acgcaggggc
     1021 gcgcgacgtc gacacgccat tcgaagtttg gccagcatct ccgcaaagag tttaatattg
     1081 gttgagtaat tggaaaagaa cgggctcaac aaagagataa ggcagcctca ctgaatccgg
     1141 aggccgtctg tcaactcttg gttaaccaaa ttctcgcggc cagcgtctca ggaagcggtc
     1201 accaccaccg cttcgccctc gggcgccagc accactttca gtccgcaggc ctcagccaga
     1261 agccgcgtgt aataaggctg taccgcatgg gcatcgacgc tcgaggtcga atccgcactc
     1321 aagagatcgg cgatgttttg cggcatccgc gcgttcagcc cggcggcact gacgcgaaag
     1381 ccgattgcat cgccctcgcc gaccggatcg acggtaagca cgccgccacg cggtatcgtc
     1441 tgctgcgcaa tcaccatcat gttcagcagc agtttgaccc ggttcttcgc aagcagtacg
     1501 cgcggcaaat tccacgcgat cgtggtcttg ctgtcttcaa gatgaccccg cgccatggtc
     1561 tgcgcatcgc ccagatcgat ctgcgcgccc gtcgatcccg cagccccgaa cgccaggcgg
     1621 cagaactgca atcgcgccga ggccgtcttg gcgctcttgc ggatcaaatc gagggcaaat
     1681 tcgcgatcgt ccggcttggg attgtcgtcg aggacttcaa gcccattgac gatggcgccc
     1741 accggactga tgagatcgtg gcaaacccgc gaacataaca gcgccgcgag ttcgagcgca
     1801 tcgggggcgg gaacgggaga cgaggtacca gacatatgat gttcctggaa agccgcgaca
     1861 ggcgcgaaca cggcgaatca cgcatgctcg ctggcttgat acactgcgga cgcttgtgct
     1921 gccaacacaa ggtgaccaga gggaaggacc gactgcaaat gaggcctgcc aatgaatgag
     1981 gcacattcgt cgagaccggc gatcgattgt gatgccgcgg ccgcgctgat ggaccccctg
     2041 accgatctgg tgatccgggc gggtgcggcc attgtggcca tcaatcgtgt cgcgatgaag
     2101 atcgacggca aggcggacgg atcgccggtg acggaagccg atctggccgc cgaccggatc
     2161 attggcgaag gcctggcgcg gctttttccg gaaataccgg cattatccga ggaacgcgtt
     2221 gatccggcca agccgcctta tcgtggcagt ttctttctga tcgacccgct tgacggcacc
     2281 aaggaatttg tcgccggccg cgacgaattt accgtcaatc tcgcgctggt gaccaacgcg
     2341 acgccgctat tgggcatcat cggcgccccg gccctcggat tgatatggcg cgggctggtg
     2401 ggctgcggcg cccaacgact gacgatagcg gaaggatcgg tgcgagatgt cgagccgatc
     2461 catacccgcc ccttcccgcg cggcggcgcc ccctggatcg cggccgtcag ccgctcgcat
     2521 ggagatacaa ggactgaggc ctttattgag gcgaggccgg gtgcgatccg gcaagcgctt
     2581 ggctcggcgg tcaaattcgg ccgcgtcgca gagggcggag ccgatattta tccccgcctc
     2641 gccccaacca gcgaatggga tatcgcggcc ggccatgcca tcgtcaccgc cgctggtggc
     2701 aagatcacgg atgcgaacgg caaggacttg cgttttggcg agcggcgcga cggctttatc
     2761 gtgccggaat tcatcgcctg gggcgatccc gcggcggtgc gctgattcgc tattgggcca
     2821 gttgttgctc caacgccggc cagcgcgagg ttgccttcct ttggaagcgc tccggatcgg
     2881 cggcgaaggc atcccggttg tcctcgcggc caaacagata aagccgttgt cccgcgatca
     2941 gccagaaccg ggggttaccg gcataggtga cgccccgcgc cagatcgacc gggtcgtagc
     3001 ctccgaactg gggcccgtaa atatccggat gggcaacaaa agaagcgcga ttgcc
//
LOCUS       DASWGM010000872         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1327257,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000872 DASWGM010000000
VERSION     DASWGM010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327257"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VGA15_23715"
     CDS             complement(<1..441)
                     /locus_tag="VGA15_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9590734.1"
                     /translation="MTAALTAEGLHTYYGKSHILHGVSLEVAEGKITALLGRNGAGKT
                     TTLRSLMGLTPAREGKITIFGEDTTHWPTFRIAAQGGVGYVPEGRRIFANLSVEENLK
                     VPLERGGPWTVERIYQLFPRLEERKLNRGRQLSGGEQEMLSIGRA"
     gene            complement(438..1187)
                     /locus_tag="VGA15_23720"
     CDS             complement(438..1187)
                     /locus_tag="VGA15_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590735.1"
                     /translation="MNVLEINNVTKRFGNLVAVREVSLSVARGELCAIIGPNGAGKTT
                     FFNLISGFFPPTSGAIIFDGRDITRLSTHQRVTLGMARTFQITEIFPELTVFENVRIA
                     AEVAGGYRLRPWINRSEQARIHARVEETLKLVSLETKTDRLVGELAHGDQRAAEIAMA
                     LALRPHLLLLDEPTAGMGDQETYQITQLIRRLHRDSNYTIVLIEHDMRVVFHLADRIC
                     VLDQGKMLAQGTPTEIAANETVQAAYLGNAA"
     gene            complement(1184..2137)
                     /locus_tag="VGA15_23725"
     CDS             complement(1184..2137)
                     /locus_tag="VGA15_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590736.1"
                     /translation="MNAEQTRKVIVLAAIWTTLVLAPYWMVPLGGYTALATRVLVLGL
                     AAMSVNFLLGFTGVLSFGHAAYFGLGAYGAGLTLKFLALSTPLALIAGTLLGGLCGAI
                     LGTLIVRRRGVYFAMVTIAFGQVFYYIAFQWSSLTGGDDGLRGFSRQPLDFGLFKLDI
                     LSSANNFYFFVLFCFALVVGAMGFILRSPFGRTMIAIRENERRSRFLGIPVERHIWIA
                     FTLSCFFMGFAGALYALVNNFADPRGLHYSQSGDFVMMAVMGGMRSFWGPLLGAAVFV
                     VLQDYLSSITVNWMSFVGLLFVLVVLFFPRGLLGFIQRSKA"
     gene            complement(2134..3003)
                     /locus_tag="VGA15_23730"
     CDS             complement(2134..3003)
                     /locus_tag="VGA15_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590737.1"
                     /translation="MTQLLLFNVTNGLIIGAFYVLMALGLSLILNLSNVINFAHGGFL
                     VIGGYIAYTITPYIGFWGALLLAPPLTAVIGLAVERVLIRPLYGRDPLYSLLLTFGLA
                     FMIEDGTRFIWGPQGKPVTIPAFLAQPLSNDLFFITGYRLFMVAVVMIAVALLFLLLR
                     YTRLGIRIRAGTLDLETVAALGINVQMLRSLNFAVGIFLAGLSGVLAAGQLGLEPTMG
                     TGLLMPSFIAIIVGGIGSLPGTLVGGLLIGVASGLTAVFLPAASEAVIYVMMAVVLLL
                     RPRGLFGEEGMMS"
     gene            complement(3081..>3969)
                     /locus_tag="VGA15_23735"
     CDS             complement(3081..>3969)
                     /locus_tag="VGA15_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9590738.1"
                     /translation="QVTSEKKILHIVSGGHTDSITGKDCKWNVFRVCNTTRMEANSVS
                     SVLYNKYGKKWHFITPDYAFGHTLYDACTDDLKKLGGTVTGNELTPLGTTDFSAYLIK
                     ARAANPDVLILLVQGSDMINCLKQVVQFGLNKQIHVAGTQQELESLAALPPEARIGVW
                     MFEWYWKQPGVPGVEKFVADIRKVNNGNVPTARHWFGYASAMSFALVANQEKALEAVK
                     LAGALENFELPDDVKLQPNKVYYRKGDHQLMTSAFVGEAQSKGKDDPEDLYRVDEVVP
                     GDKTAPAVSETGCIVQWPA"
BASE COUNT          826 a         1209 c         1234 g          700 t
ORIGIN      
        1 cgcgcggccg atcgacagca tctcctgctc gccaccggaa agttgccggc cgcgattgag
       61 cttgcgctcc tccaggcgag ggaacagctg gtagatccgc tcgaccgtcc aggggccgcc
      121 gcgctcgagc ggaactttca gattttcctc gacgctgaga ttggcgaaga tgcgccggcc
      181 ctcgggcaca tagccgacgc cgccctgggc tgcgatccgg aaggtcggcc aatgcgtggt
      241 gtcctcgccg aagatggtaa tcttgccttc ccgcgccggc gtcaggccca tcagacttcg
      301 cagcgtcgtg gtcttgcccg cgccgttgcg tccaagcagc gcagtgatct tgccctcggc
      361 gacctcgagg ctgacgccat gcaggatatg gctcttgccg tagtaagtgt ggaggccctc
      421 ggccgtcagt gctgcggtca tgcggcgttg cccagatagg cggcctgaac ggtctcgttg
      481 gcggcgatct ccgtgggcgt gccctgcgcc agcatcttgc cctggtcgag cacgcagatg
      541 cggtcggcga gatgaaatac cacgcgcatg tcgtgttcga tcagcacgat ggtgtagttg
      601 ctgtcgcggt gcagccggcg gatcagctgg gtgatctggt aagtctcctg gtcgcccatt
      661 ccggcggtcg gctcgtccag cagcagcaga tgcggtctta atgccagcgc catcgcgatc
      721 tcggcggcgc gctgatcgcc gtgcgcgagt tcgccgacca gtcgatcggt cttggtctcc
      781 agcgatacca gtttcagcgt ctcttcgacc cgtgcatgga tccttgcctg ttccgaccgg
      841 ttgatccagg gccgcaaccg atatccgccg gccacttccg cggcaatgcg gacgttctcg
      901 aacacggtga gttccggaaa gatctcggtg atctggaacg tccgggccat gccgagcgtg
      961 acgcgctgat gggttgaaag tctggtgatg tcgcggccat cgaagatgat ggcgccggag
     1021 gtcggcggaa aaaacccgga gatcaggttg aagaaggtcg tcttgcccgc gccgttcggt
     1081 ccgatgatcg cgcacaattc tcccctcgcg acgctgagcg agacttcacg caccgccacc
     1141 aggtttccga accgcttggt gacattgttg atttcgagaa cgttcatgcc ttgctccgtt
     1201 gaatgaagcc gagcaggcca cgcgggaaaa acaggacgac gaggacgaac agcagtccga
     1261 cgaacgacat ccagttgacg gtgatgctcg acaggtagtc ctgcagcacc acgaacacgg
     1321 cggcgccgag cagcgggccc caaaaactgc gcatgccgcc catcaccgcc atcatgacga
     1381 aatcgccgga ttggctgtaa tgcaggcccc tcggatcggc aaaattgttg accagcgcat
     1441 agagcgcgcc ggcaaatccc atgaagaagc aggacagggt gaaggcgatc cagatgtgtc
     1501 gttccaccgg gatgccgagg aagcgcgagc ggcgctcgtt ctcgcggatc gcgatcatgg
     1561 tgcggccgaa cggcgagcgc aaaatgaatc ccatggcgcc gaccaccagg gcaaaacaga
     1621 acagcacgaa aaagtagaag ttgttggcgc tggagagaat gtcgagcttg aacaggccaa
     1681 agtcgagcgg ctggcgtgag aagccgcgca agccatcgtc gccacctgtc agcgaactcc
     1741 actgaaaggc gatatagtaa aacacctggc cgaacgcgat cgtcaccatg gcgaaataga
     1801 cgccgcgccg ccgcacgatc agcgtgccga ggatggcgcc gcacaggccg ccaagcagcg
     1861 ttcccgcgat cagcgccagc ggcgtgctca gggccaggaa tttgagcgtc aaaccggcgc
     1921 cgtaggcacc gagaccgaaa taggcggcgt gaccgaacga cagaacgccg gtaaatccca
     1981 gcaggaaatt caccgacatc gctgccagtc caagtaccag gacccgggtg gcgagcgcgg
     2041 tgtaaccgcc gagcggcacc atccaatagg gagcgagcac aagcgtggtc cagatcgccg
     2101 caaggacgat cactttacgc gtctgttcgg cgttcaactc atcattcctt cttcgccgaa
     2161 cagtccgcgc ggtcgcaaca gcaggaccac cgccatcatg acgtagatca ccgcctcgct
     2221 ggcggccggg agaaataccg ctgtcaatcc cgacgccacc ccgatcagca ggccgccgac
     2281 cagcgttccc ggcaggctgc cgatgccgcc gacgatgatg gcaatgaagc tcggcatcaa
     2341 aaggccggtg cccatggtcg gttcaagacc gagttgtccg gccgcgagaa ccccgctgag
     2401 gccggccaga aaaatcccga ccgcgaaatt gagcgagcgc agcatctgca cgttgattcc
     2461 gagcgccgcg acggtctcga ggtcaagcgt gccggcccgg atgcgaatgc ccaaccgggt
     2521 gtagcgtaag agcaggaaca ataatgccac cgcgatcatc accaccgcca ccatgaacag
     2581 gcgatagccg gtgatgaaaa acagatcgtt gctcaaaggc tgcgccagaa aggcgggaat
     2641 ggtgaccggc ttgccctgcg gaccccagat gaagcgcgtg ccatcctcga tcatgaaggc
     2701 gagaccgaag gtgagcagca ggctatagag cggatcgcgt ccatagagcg ggcggatcag
     2761 cacgcgttcg acggctagtc cgatgacggc agtcagcggc ggcgccagca gcagcgcgcc
     2821 ccagaaaccg atatagggcg tgatggtgta ggcgatgtag ccgccgatga cgagaaatcc
     2881 gccatgcgcg aaattgatga cgttgctcag gttgagaatg agagagaggc cgagcgccat
     2941 cagcacgtag aaggcgccga tgatgagccc attggtgacg ttaaacagga gcagttgcgt
     3001 catagaccac cggatgagag gagcacacgt ctgatccggg aggcgtggcc tcccggacct
     3061 cagaaatcgc cgacctcatg ctaagccggc cactgaacga tgcagccggt ctcgctgaca
     3121 gcaggcgccg tcttgtcgcc cggcacgact tcgtccacac ggtacagatc ttccggatcg
     3181 tccttgccct ttgactgggc ctcgccgaca aaggccgacg tcatcaattg atgatcgccc
     3241 ttgcggtagt agaccttgtt cggctgcagc ttgacatcat cgggcagctc gaagttttcg
     3301 agcgcgccgg caagcttgac cgcctcgagc gccttctcct ggttcgcgac cagggcaaag
     3361 ctcatggccg acgcatagcc gaaccagtgg cgcgcggtcg gtacattgcc gttgttgacc
     3421 ttgcgaatat cggcgacgaa tttttcgaca ccgggaacgc ccggctgttt ccagtaccat
     3481 tcgaacatcc agacgccgat ccgtgcttcc ggcggcagcg cggcgagcga ttccagctcc
     3541 tgctgcgttc ccgctacgtg gatctgcttg ttgaggccaa actgcacgac ctgcttcagg
     3601 caattgatca tgtcggagcc ctgcaccagc aggatcagca cgtccgggtt cgccgcccgc
     3661 gccttgatca gataggcgga aaaatccgtg gtgccgagcg gcgtcagctc gttgccggtg
     3721 acggtgccgc cgagcttctt caaatcatcg gtgcaggcgt cataaagcgt atgaccgaag
     3781 gcgtagtccg gggtgatgaa gtgccatttc ttgccatact tgttgtatag cacgctggat
     3841 accgaattgg cttccatccg cgtggtgttg cagacgcgga acacattcca cttgcaatcc
     3901 ttgccggtaa tcgaatcggt atggccgccg gaaacgatgt gcaggatttt cttttcgctg
     3961 gtgacttgc
//
LOCUS       DASWGM010000873         4212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1327328,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000873 DASWGM010000000
VERSION     DASWGM010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327328"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VGA15_23740"
     CDS             <1..310
                     /locus_tag="VGA15_23740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590739.1"
                     /translation="TDTPGLTFTAPHSYSTVKTIARGMPGDSGVTVVTNARAFYTTRA
                     AAGASGARHSLRPLIGEGGKFTAKLGRGARRDREAVFANEAASANNVLFENLCSAAIH
                     "
     gene            complement(491..1183)
                     /locus_tag="VGA15_23745"
     CDS             complement(491..1183)
                     /locus_tag="VGA15_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEX9590740.1"
                     /translation="MSNAPLMPKATAVWLVDNTALTFDQVADFTKMHPLEVRAIADGD
                     AAQGIKGMDPISTGQLTRDEIERGEKDVNYRLKLGESKVTLPPAAKKKGPRYTPVSRR
                     HERPSAILWLVRNHPELKDAQIMRLVGTTKTTIASVRDRTHWNASTLTPMDPVTLGLC
                     SQIELDFEVQRAAKEKPTDTAYGGATLLPASETTRKEPEYEPTEKQRDDLNVDAVFAK
                     LKTIGGKKQDEE"
     gene            1399..2364
                     /gene="ispH"
                     /locus_tag="VGA15_23750"
     CDS             1399..2364
                     /gene="ispH"
                     /locus_tag="VGA15_23750"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX9590741.1"
                     /translation="MPAKPPLRIVLCSPRGFCAGVVRAIDTVERALTIYGAPVYVRHE
                     IVHNRYVVDSLKTKGAIFVEELAEIPDNTNAPVVFSAHGVPKSVPADARARNFFSLDA
                     TCPLVTKVHREAAIHFKRGREILLIGHSHHPEVVGTLGQLPQGAVTLIETAHDAATFT
                     PKDPNNLAFVTQTTLSIDDTAEIVAMLKERFPNISGPHKEDICYATTNRQLAVKKVAP
                     VVDALIVVGAPNSSNSQRLREVAEREGCPVSVLAQRAGDLDWSMFEGITSLGITAGAS
                     APEVIVEEIMGAFAERYELHVETVSAAEENEFFPLPRSLRPDAAE"
     gene            2370..3365
                     /locus_tag="VGA15_23755"
     CDS             2370..3365
                     /locus_tag="VGA15_23755"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX9590742.1"
                     /translation="MAVYTDVAADELAEFLSRYDIGELQSYKGIAEGVENSNFLLHTT
                     AGYFILTLYEKRVAKGDLPFFLGLMTHLAGHGINCPQPVQNKSGEALSTLAGRPVAII
                     TFLEGVWPRKPNAAHCAGVGQALAKMHLAGRDFGMTRANALSVSGWRPLFDQASSRAD
                     ELQHGLRAFIGAELDHLESNVWPGNLPKNLPQGVIHADLFPDNVFFLGERVSGIIDFT
                     FACTDMLAYDVAICLNAWCFESDYSFNVTKARAFLGAYGRERQLSDAEQDALPLLARG
                     AALRFLLTRLVDFLNVPTGALVRPKDPLEYVRKLRFQQGVKSMRDYGVAASGLVA"
     gene            3362..3814
                     /gene="rnhA"
                     /locus_tag="VGA15_23760"
     CDS             3362..3814
                     /gene="rnhA"
                     /locus_tag="VGA15_23760"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX9590743.1"
                     /translation="MSSLPSVTIHTDGACSGNPGPGGWGAILKFGDVEKELKGGEAHT
                     TNNRMELMAAISALETLKKPCTVDLYTDSQYVRQGITGWIHGWKKNGWRTSDKKPVKN
                     VDLWQRLDASLHQHQVRWHWVKGHAGHDENERADQLAREGVAMARLKQ"
     gene            complement(3970..>4212)
                     /locus_tag="VGA15_23765"
     CDS             complement(3970..>4212)
                     /locus_tag="VGA15_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX9590744.1"
                     /translation="VMNAWKRDTDQRDEATFLADGNAEFTKAIGMELDASGNGLGIRS
                     KRYSMLVDDGVVKKFNLEPAPGKVEVSGGDTLLGQL"
BASE COUNT          740 a         1318 c         1336 g          818 t
ORIGIN      
        1 aacagatacg ccgggcctaa cgttcactgc gccacactcc tactctaccg ttaaaaccat
       61 tgcgcgggga atgccgggtg attccggtgt gaccgtggtg actaacgcgc gtgctttcta
      121 cactacacgc gcggctgcgg gtgcatcggg cgcccggcat tccctgcgcc ctctgattgg
      181 agagggcggg aagttcacag caaaactcgg gcgcggtgcg cggcgagatc gcgaagctgt
      241 gtttgcaaac gaagctgcgt ctgcaaacaa cgtgctgttt gaaaatttat gctctgcggc
      301 aattcattga atacaagcgc gccacacgcg tgcccgtcac cacccgcgaa ggcgggtggc
      361 ccagtattcc agagggtcag tgatggagcc gaacagtcgc ggcgtactgg atccccgcat
      421 gcgcggggat gaccagcgtt gctggggcgg gccagtgcgc tatcgcatcc agaccgcaga
      481 atggatccgt ttattcctcg tcctgcttct tgccgccgat ggtcttcagc ttggcaaaca
      541 ccgcatcgac attgaggtcg tcgcgctgtt tttcggtcgg ctcgtattcc ggttctttcc
      601 gggtggtctc ggaggccggc agcaaagtgg cgccgccgta ggcggtgtcg gtcggcttct
      661 ccttggcggc gcgctgcacc tcgaagtcga gctcgatctg cgagcacagg ccgagcgtca
      721 ccgggtccat cggggtcagg gtcgaggcat tccagtgggt gcggtcgcgg acgctcgcaa
      781 tggtggtctt ggtggtgccg accaggcgca tgatctgcgc gtccttcaat tccgggtgat
      841 tgcgcaccag ccacaggatc gcgctcggcc gctcgtggcg gcgcgacacc ggggtgtagc
      901 gcgggccctt cttcttggcg gccggcggca gcgtcacctt gctctcgccg agcttgaggc
      961 ggtagttcac gtccttctcg cccctttcga tctcgtcgcg ggtgagctgg ccggtcgaga
     1021 tcgggtccat gcccttgatg ccctgggcgg cgtcgccatc ggcgatggcg cggacctcga
     1081 gcgggtgcat cttggtgaaa tcggccactt gatcgaaagt cagggcggta ttgtcaacca
     1141 gccacacggc agtcgccttc ggcatcagcg gtgcattgct catggcatat ctcctaagcg
     1201 cttcgcccac cccttttgga ggcgaagccg tggtcatcag cgatgacggg aattatcccc
     1261 tatataagcc gtctggggcc acccgcgcaa tggtttgctg accggccttg acagcgcccg
     1321 aaagccgtcc caagtcctta tacgaattga ccgttccctc cgtgccggtg aggggtgatt
     1381 cggtccgaga tcgcccttat gccagccaaa ccacccctta gaatcgtact ttgttcgcca
     1441 cggggctttt gcgccggcgt ggtgcgggca atcgacaccg tggaacgggc gctcaccatc
     1501 tatggcgccc cggtctatgt ccgccacgag atcgtccaca accgctatgt cgtcgacagc
     1561 ctcaagacca agggcgcgat tttcgtcgag gaactggccg aaatccccga caataccaac
     1621 gcgccggtgg tattttcggc ccatggggtt ccgaaatcgg tcccggccga tgcccgggcg
     1681 cgcaatttct tttcgctcga cgccacctgt ccgctggtca ccaaggtgca ccgcgaggcg
     1741 gcgatccatt tcaagcgcgg ccgtgaaatc ctgctgatcg ggcattccca ccatccggaa
     1801 gtggtcggca cgttaggaca gcttccacaa ggagcggtga cgctgatcga gaccgctcac
     1861 gatgccgcga ccttcacgcc gaaggatccc aacaatcttg ccttcgtcac ccagacgacg
     1921 ctgtcgatcg acgacaccgc cgagatcgtc gcgatgctga aggagcggtt tcccaacatc
     1981 tccggcccgc acaaggaaga catctgctac gccaccacca accgccagct cgcggtgaaa
     2041 aaggtggcgc cggtggtcga cgccctgatc gtggtcggcg cgccgaactc gtcgaattcg
     2101 cagcggctgc gcgaagtcgc cgagcgcgag ggctgcccgg tctcggtatt ggcgcagcgc
     2161 gccggcgatc tcgactggag catgttcgag ggcatcacga gcctcggcat caccgcgggc
     2221 gcgtcggcgc cggaagtgat cgtcgaggag atcatgggtg cgttcgccga gcgttacgaa
     2281 ttgcatgtgg agacggtctc ggccgcggaa gagaacgaat tctttccgtt gccgcgctcg
     2341 ctgcggcccg acgcggcgga ataatcgcaa tggcggttta taccgacgtc gccgccgacg
     2401 agcttgcgga gtttttgagc cgttacgaca tcggcgaatt gcagtcctac aagggcatcg
     2461 ccgagggcgt ggaaaattcc aatttcctgc tgcacaccac cgccggctat tttattctga
     2521 cgctgtacga gaagcgcgtc gccaagggcg acctgccgtt ctttctcggc ctgatgacgc
     2581 atctggccgg ccacggcatc aattgtccgc agccggtgca gaacaaaagc ggcgaagccc
     2641 tgagcacgct ggccggccgt ccggtggcga tcatcacttt tctggaaggc gtctggccgc
     2701 gcaagcccaa cgccgcgcac tgtgccgggg tgggtcaggc gctggcgaag atgcatctgg
     2761 cggggcgcga tttcggcatg acgcgcgcca acgcgctgtc ggtgtcgggc tggcgcccgc
     2821 tgttcgatca ggcctcatca cgcgccgatg aactgcagca tggcttgcgc gcgtttatcg
     2881 gcgccgaact cgatcatctc gaaagcaacg tctggcccgg gaatctgccg aagaatttgc
     2941 cgcaaggggt gatccacgcc gatctgtttc cggacaatgt cttcttcctc ggcgaacgcg
     3001 tgtccggcat catcgatttc accttcgcct gcaccgacat gctggcctac gacgtcgcca
     3061 tctgtctcaa cgcctggtgt ttcgagagcg attattcgtt caacgtcacc aaggcgcgcg
     3121 ccttccttgg cgcctatggc cgcgagcgcc aattgtccga cgccgagcag gatgcgctgc
     3181 cgttattggc ccgcggtgcg gcgttgcgct tcctgctgac gcgcctggtc gatttcctca
     3241 acgttcccac cggcgcgctg gtgcggccaa aggatccgct cgaatatgtc cgcaagctgc
     3301 gctttcagca gggcgtgaag agcatgcgcg attacggcgt cgcggcctcg gggctggtgg
     3361 cgtgagttca ctcccgtcgg tcaccattca caccgatggc gcgtgctcgg gcaatcctgg
     3421 gcccggcggc tggggcgcga tcctgaaatt cggcgatgtc gagaaggaat tgaagggcgg
     3481 cgaggcgcat accaccaaca accgcatgga gctgatggcg gcgatctccg cgcttgaaac
     3541 attgaagaag ccgtgcaccg ttgatctcta caccgacagc cagtatgtcc gtcagggcat
     3601 caccggctgg atccatggct ggaagaagaa cggctggcgc acctcggaca agaagccggt
     3661 caagaacgtc gacctgtggc agcggctgga tgcgtcgctg catcagcacc aggtgcgctg
     3721 gcactgggtc aagggccacg ccggccacga cgagaacgaa cgcgccgatc aactggcgcg
     3781 ggagggcgtc gccatggcgc ggctgaaaca gtagctggag gatcagggcg gatgctccac
     3841 gtactgccgt catcacccgc gaaagcaggt gacccagtat tccagagttt tcgtgataaa
     3901 ccgcgagctc tcggagtact ggatcgcccg gtcaagccgg gcgacgacaa gtgttgtttg
     3961 ggccggctcc tacagctgcc ccagcaacgt atcgccgccg gaaacctcga ccttgcccgg
     4021 cgccggctcg aggttgaact tcttgaccac gccgtcatcc accagcatcg aatagcgctt
     4081 ggagcggatg ccgaggccgt tgccggaggc gtcgagctcc atgccgatcg ccttggtgaa
     4141 ttcggcgttg ccgtcggcga ggaaggtggc ttcgtcgcgc tggtcggtgt cgcgcttcca
     4201 cgcgttcatg ac
//
LOCUS       DASWGM010000874         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1066031,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000874 DASWGM010000000
VERSION     DASWGM010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066031"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="VGA15_23770"
     CDS             <1..1845
                     /locus_tag="VGA15_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9590745.1"
                     /translation="AAADRDLLVSAQTGSGKTVAYGLAIAKKLLDGAGRFGRAAAPLA
                     LIVAPTRELALQVQRELAWLYQYADARVVSCVGGMDPRREQRELAEGAHIVVGTPGRL
                     CDHLRRGRLDVSELKAVVLDEADEMLDLGFREDMEFILKTTPGTRRTLLFSATLPRGI
                     VALAKQYQQQAFRVEVAGDEGGHADIEYRAIRIAAGDVEHAVVNVLRFFESPSALVFC
                     NTRHAVRHLQATLLERGFSVVALSGELTQNERTQALQALRDGRARVCVATDVAARGID
                     LPNLDLVIHADLPNDPEVMQHRSGRTGRAGRKGVSVLLVPAARRRRAELLLNLAGIDV
                     VWGTAPTADEIRKLDQERMLRDALFAEETTSDDLILARALLAERSPEDIATALARLYR
                     ARLPSPEDILDPGQGAGRSRDSSGREKDGRASRGDDRATAPRSKTAKTSPRHGMADGS
                     VWFRAAIGRQKNAEARWLLPMICRRGCIDKHDIGAIRILETTTEFEISKAVAEAFAGK
                     IRRPDKEDNIRIEPLADAPQSLAPSEKRLHAPRHEGKEADRSARQNDRYRDGPKQNGK
                     FHNASGSRFDKEAGSRTKQRHQGRQPQLAGASLAKPAFGKKAKKRRRG"
     gene            1898..2125
                     /locus_tag="VGA15_23775"
     CDS             1898..2125
                     /locus_tag="VGA15_23775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590746.1"
                     /translation="MMKNAFSGEIDRFGTVRMPQTSWGQAQAVPNHSSYRLPANTALR
                     KIKARTKPRQPIVAPATTPTSKLHRMSVTGR"
     gene            complement(2217..2636)
                     /locus_tag="VGA15_23780"
     CDS             complement(2217..2636)
                     /locus_tag="VGA15_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590747.1"
                     /translation="MPLARYFLFVGGVLLALLLLSNEVLPKLPVEAERAEAGPSKSGI
                     RIQSDRKWPERVVFDTNLPTIVPPQTATIAVSAPAQTTVADVSAKSRALQSFAELKPS
                     EPKKPVHKRRIAKVRVAPPMVMVAQQPRFGFFDNNTW"
     gene            complement(2772..>3266)
                     /locus_tag="VGA15_23785"
     CDS             complement(2772..>3266)
                     /locus_tag="VGA15_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590748.1"
                     /translation="ASWMLMSGSSSKTAKSEPARLVTAAAPQPPPSPATNIPQEGFPP
                     NPATAPVETTATLPLEAIAPVEAAPVDGLKISSQYWRRGGLGSKALVTFTLRNGNGYP
                     VKDIEISCAFARRDGSHLTDRKRTIHDTLNMKSRKTFARLHVGFVNVNADRAKCSLVT
                     ASRV"
BASE COUNT          611 a         1017 c         1048 g          590 t
ORIGIN      
        1 gccgctgccg accgcgacct gctggtttcg gcgcaaaccg gctcgggcaa aactgtcgcc
       61 tatgggctgg ccatcgcaaa gaagcttctt gatggtgccg ggcggttcgg gcgagctgcg
      121 gcaccccttg cattgatcgt cgcgccaacg cgcgaactcg cacttcaggt gcagcgcgaa
      181 ctggcgtggc tctatcaata tgccgatgcg cgcgtcgttt cctgcgtcgg cggcatggat
      241 ccgcgccgcg aacagcgcga gcttgccgag ggcgcccaca tcgtggtcgg cacgccgggg
      301 cggctatgcg atcacctgcg gcgcggccgt ctcgacgtct cggaattgaa ggccgtcgtg
      361 ctcgacgagg ccgacgagat gctcgatctc ggttttcgcg aggatatgga atttatcctc
      421 aagaccacgc cgggcacccg ccgcacgtta ctgttttcgg cgactttgcc gcgcggcatc
      481 gtcgcactgg caaagcaata tcaacaacag gcctttcgcg tcgaggtcgc gggcgacgaa
      541 ggtggccacg ccgatatcga gtaccgagct atccgtatcg ccgccggcga tgtcgagcac
      601 gcggtcgtca acgtgctgcg attttttgaa tcaccgagcg cgctggtgtt ctgcaacacc
      661 agacacgctg tccggcattt gcaggcgaca cttctggagc gcggcttctc cgtggtcgcg
      721 ctatcggggg aattgaccca gaacgagcga acccaggcgc tgcaggcctt gcgggatgga
      781 cgagcccgcg tctgcgtggc caccgacgtg gcggcgcgtg gcatcgatct gccgaacctt
      841 gacctcgtca ttcacgcaga tctgccgaat gacccggagg tcatgcaaca tcgctccggc
      901 cgcaccggcc gtgcgggccg caagggcgtc agcgtcctgc tggtgcctgc tgcgcgacgg
      961 cggcgtgcgg aattgctgct taatctggct ggcatcgacg tcgtctgggg cacagctccg
     1021 acggcggatg aaattcgcaa gctcgatcag gagcgcatgc tgcgcgatgc gctgttcgcg
     1081 gaggagacga catcagacga tctcattctg gcgcgggcgt tgctcgccga acggtcgccc
     1141 gaggatattg ccacggcgct ggcgcggctg tatcgggcgc ggcttccctc gcccgaggac
     1201 attctcgacc ccggtcaggg cgctggccga tcccgtgaca gcagcggccg ggaaaaagac
     1261 ggccgggcat cgcgcggcga cgatcgcgcg accgcgccac gatcgaagac ggcaaaaaca
     1321 tcgccgcgtc acggcatggc ggacggcagc gtgtggtttc gcgccgccat cggacggcag
     1381 aaaaacgcgg aggcgcgctg gctcttgccg atgatttgcc gtcgtggctg tatcgacaag
     1441 catgacatcg gcgccatccg cattctcgaa acaaccaccg agttcgaaat ttccaaagcg
     1501 gtcgccgaag cgttcgccgg caaaatccgg cgccccgaca aggaagacaa catccgcatc
     1561 gagccgctgg cagatgctcc ccagagcctg gcgccttcgg aaaagcggtt gcacgcgcct
     1621 cgacacgaag gcaaggaggc cgaccggagc gctcgtcaga acgatagata tcgagatggg
     1681 ccgaagcaaa acggcaaatt tcacaacgca agcggctcga gatttgacaa ggaagcgggt
     1741 tccaggacga aacaacggca tcagggcaga caaccgcagc ttgccggcgc atcactagcg
     1801 aaacctgcat tcggaaagaa ggccaagaag cgtcgtcgcg gctgatgctt gagcgcccgg
     1861 cccgctggcc ccgctgtcgg attggcgcgc ttttgcgatg atgaaaaacg ccttttccgg
     1921 cgagatcgac aggttcggaa ctgttcgcat gccgcagacc tcctgggggc aggcgcaagc
     1981 cgttcccaac cactcaagct accggctgcc ggcgaacacc gcattgcgca agatcaaggc
     2041 acgcacgaaa ccccggcagc cgatcgtcgc cccggcaacg acgcctacat caaaactgca
     2101 tcggatgagc gtgacgggcc gctgaacact caaatcaaag catctgccgc ggcctgctgg
     2161 gattcgttgg aaacccgctt caccgggtcg ccctccgagc gcgccagcga gctggtttac
     2221 caggtattgt tgtcaaaaaa gccaaatcgc ggttgttggg cgaccatcac catcggcggc
     2281 gccacgcgga cttttgcgat tctgcgcttg tgcaccggct tcttcggttc ggacggcttg
     2341 agctcggcaa atgactgcag cgcgcgcgac ttggctgaga cgtcggcgac cgtggtctgg
     2401 gctggagcac tgaccgctat cgttgccgtc tgcggaggaa cgatcgtcgg aaggttggta
     2461 tcgaaaacca cccgttcagg ccatttccga tccgattgga tcctgatgcc ggatttggat
     2521 gggcccgcct ccgccctctc cgcctcgacc ggcaatttcg gcagcacttc attgctgaga
     2581 agcagcagcg cgagcagaac tccgccaacg aacaaaaaat atcgcgctaa gggcatttca
     2641 gggtcgctcc ctccgcgcgg gcgcgcggac cattcactta actggggata gcgcgatcgg
     2701 ttcctagggt tgccgtgccc acgtaacggc gatgttaccg ggtgtggtta cccctcggct
     2761 gatagccgcc gctacacccg gctggcggta accagcgagc acttggcccg gtcggcgtta
     2821 acgttgacga agccgacgtg caggcgggca aaggttttgc ggctcttcat attgagggtg
     2881 tcgtggatcg tccgtttgcg gtctgtcaaa tggcttccgt cccggcgggc aaaggcgcag
     2941 gaaatctcga tatccttgac cgggtaacca tttccgttgc gcagcgtgaa tgtgaccagg
     3001 gctttcgatc ccagtccgcc ccggcgccag tattgcgatg aaatcttgag gccgtcgacc
     3061 ggagccgctt ccacaggtgc gatcgcttcc aagggcagtg tcgctgtcgt ttccacgggc
     3121 gcagtcgccg gattcggcgg gaatccttcc tgggggatgt tcgtggcggg cgatggcggc
     3181 ggttgcggcg ctgcggccgt taccaggcgc gccggttcgc tcttggcagt tttgctggaa
     3241 gagccggaca tcagcatcca cgacgc
//
LOCUS       DASWGM010000875         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2189160,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000875 DASWGM010000000
VERSION     DASWGM010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189160"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VGA15_23790"
     CDS             complement(<1..456)
                     /locus_tag="VGA15_23790"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9590749.1"
                     /translation="MTAINNSRLIARSPFARPLPPVSAILSHPQRNTIRLQLKQNLIL
                     REMPASAWNDLEPQLEIAEYRKGEPLVRQGSTNMEQFFILDGILKRVVSNPQGKEMIL
                     RFAAETDMDTSYAAWRMKTPIPYSIAAVTKVRVARLSMPHWVEFLESHTE"
     gene            548..>1794
                     /gene="oxc"
                     /locus_tag="VGA15_23795"
     CDS             548..>1794
                     /gene="oxc"
                     /locus_tag="VGA15_23795"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEX9590750.1"
                     /translation="MGTVEPQVASGQDVKVVSSGQIVANAPAQDVTDGFHLVIDALKL
                     NGIDTIFGLPGIPITDFTRMAQAAGMRVISFRHEQNAGNAAAIAGFMTQKPGICLTVS
                     APGFLNGLTALANATTNCFPMILISGSSEREIVDLQQGDYEEMDQLAVAKPLCKAAFR
                     VLQAEDIGVGIARAIRAALSGRPGGVYLDLPAKLFAQTMDAEAGKNSLIKVVDPAPKQ
                     IPAPEAVKRALDLLKGAKKPLVILGKGAAYAQADAEIRSFIEKTGLPYLPMSMAKGLL
                     PDTHPQCASAARSFVLPEADAVMLVGARLNWLLSHGKGKTWGGKGHKDWGGQKFIQID
                     ISPQEMDSNVRIDAPVVGDIGSCVSALLAGIGSNWPKPPAEWLGAIAERKSKNIAKMA
                     ETLAKDPTPMNFHSALAVVRDIV"
BASE COUNT          327 a          576 c          573 g          318 t
ORIGIN      
        1 ttcggtatgg ctttcgagga actcgaccca gtggggcatg gacaggcgcg ccacgcggac
       61 cttggtgacc gctgcgatgc tgtaggggat gggggttttc atccgccacg cggcgtagga
      121 ggtgtccatg tcggtctcgg ccgcgaagcg caggatcatc tcctttccct gaggattgct
      181 cacgacgcgc ttcaggatcc cgtccaggat gaagaactgc tccatgttgg tgctgccttg
      241 gcgcaccagc ggctcgcctt tcctgtactc cgcgatctcc agctgcggct ccagatcgtt
      301 ccaggcggac gccggcattt cgcgcaggat aaggttctgc ttgagctgca ggcgtatcgt
      361 gttgcgctgg gggtggctga ggatggccga cacgggtggc agcgggcgtg cgaacggtga
      421 gcgggcaatt aagcgggaat tattgatcgc agtcaaggtt gaacttcgtc agcaatttta
      481 gactcttgag tcgatttttg ccctcgcccg agcactgggc tgaagggcgg catgcaagga
      541 gacctccatg ggaacagtcg agccgcaagt cgcgtccggt caggacgtga aggtggtgag
      601 cagcggccag atcgtcgcca atgcgccagc acaagatgta accgacggct ttcatctggt
      661 catcgatgcc ctaaaactca acggcatcga cacgatcttc ggtctgcccg gtatcccgat
      721 taccgacttc acccgcatgg cacaggccgc aggcatgcgc gtgatttcgt tccgccatga
      781 gcagaacgcc ggcaacgccg ccgccatcgc cggcttcatg acgcaaaagc cgggaatctg
      841 cctgacggtg tccgcccccg gcttcctcaa cggcctgacc gcactggcca acgccaccac
      901 caattgcttc ccgatgatcc tcatcagcgg ctccagcgag cgcgagatcg tcgacctcca
      961 gcagggtgac tacgaagaga tggaccagct ggccgtcgcc aagccgctgt gcaaggccgc
     1021 cttccgcgtg ctgcaggccg aggacatcgg tgtgggcatc gcgcgcgcta tccgcgccgc
     1081 gctctccggc cgtcccggtg gcgtctacct cgacctgccg gcgaagctgt tcgcacagac
     1141 catggatgcc gaggccggca agaattccct catcaaggtg gtggatcccg cgcccaagca
     1201 gattccggcg ccggaagccg tcaagcgcgc gctcgatctg ctgaaggggg cgaagaagcc
     1261 gctggtcatc ctcggcaaag gcgcggccta tgcgcaggcc gatgcggaga tccgttcctt
     1321 catcgagaag acgggcctcc cgtacctccc catgtccatg gcaaaggggc tattgcccga
     1381 tacgcatccg cagtgcgctt cagccgcccg ctcgttcgtg ctgccggagg ccgacgcggt
     1441 gatgctcgtc ggtgcgcgcc tcaactggct gctctcgcat ggcaagggca agacctgggg
     1501 cggcaagggc cacaaggact ggggcggcca gaagttcatc cagatcgaca tctcgccgca
     1561 ggagatggac agcaacgtcc gcatcgacgc cccggtggtc ggcgacatcg gttcctgcgt
     1621 gtccgcgctg cttgccggga tcggctccaa ctggcccaaa ccgccggccg agtggctcgg
     1681 cgccatcgcc gagcgcaaga gcaagaacat cgccaagatg gccgagacgc tggccaagga
     1741 tccgacgccg atgaacttcc acagcgcgct cgccgtggtg cgcgacatcg tcaa
//
LOCUS       DASWGM010000876        10510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1328307,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000876 DASWGM010000000
VERSION     DASWGM010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328307"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..667
                     /locus_tag="VGA15_23800"
     CDS             2..667
                     /locus_tag="VGA15_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX9590751.1"
                     /translation="MAEQGDVSRGQQDFRACAPCHSLEPDRNMTGPSLANLWGRKAGG
                     LSSFERYSEALKASGIIWDDRSLDGWLTDPDHMVPDNEMPFNGIKDTRARADLLAFLR
                     DATKPGAPQQTVQQGGMKGMGGMMGGGMMGGGGDPNLKDLEPAKQVKAITHCRDTYRV
                     TTADGKTRAFWERNLRFMTDSSKDGPEREVPAIMPAGMMGDRAAVIFAAPEEIARMIE
                     ARC"
     gene            836..1225
                     /locus_tag="VGA15_23805"
     CDS             836..1225
                     /locus_tag="VGA15_23805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590752.1"
                     /translation="MKRNLAIRRLLATLVVAGLALAPVSRPVMAETASDASMQAVADE
                     MAPSSATTDEMAGDMPCCPSKAPSPVGCDKCVYMTACVSKCFTGMSSIVLHPFLTASN
                     DIASLQNDAWPDGLGHPPPEHPPRILV"
     gene            1323..1730
                     /locus_tag="VGA15_23810"
     CDS             1323..1730
                     /locus_tag="VGA15_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX9590753.1"
                     /translation="MKTFKLSRALRAALIGIAMIGSAGIARADIKDYEFQLIDQTVKA
                     GSDKTITVRLVNKATGKSVPDAVIFATRLDMAPDGMQEMATKIAPITSAEPGTYKFKA
                     TFGMAGRWQLSLGAKVQGETGTVESKLVITAQK"
     gene            1727..3133
                     /locus_tag="VGA15_23815"
     CDS             1727..3133
                     /locus_tag="VGA15_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9590754.1"
                     /translation="MKRAIAGVTVAALIAAAGGGFIGVRRHLHLTAAPLVSTALAQTS
                     DDPIYYQDPDGKPFYSLTPKKVPDGRDYRAVPAGADLSFDDEPAAAPATAAATDRKIK
                     FYRNPMGLPDTSPTPKKDSMGMDYIPVYEGEDTDDGSVKLSPGKIQRTGVKSEPASQR
                     IIRTAIRAPGTIQLDERRASIVSFRFEGFIERVENVTTGEHVHKGQALMRVYSPSLSS
                     AAAEYLSALNAKADSGVSAQALKGARRRLENLGMPEKAIAEIERTREISLSIPWLAPQ
                     DGEILERAAVNGMRAAPGDMLFRIADHQVVWVLVDIAERDLEGIAIGQKVDVRPRAFA
                     NRVFSGDVALIYPHLNAQTRTARVRIELANPDGLLRPDMYVDAEIDTGSPQPVLAVPE
                     SAILDTGSRQAVFVDKGQGRFEPRDVKLGHRGGGYVEIRQGVTEGEPVVVSANFLIDA
                     ESNLMAALKGFSDAGAQP"
     gene            3130..6324
                     /locus_tag="VGA15_23820"
     CDS             3130..6324
                     /locus_tag="VGA15_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX9590755.1"
                     /translation="MIARLIAWSARNLLLVFFGAGFAAAAGVYALAHLPLDAIPDLSD
                     TQVIVYTEYPGQAPQVIEDQVTYPLTTAMLTVPRSKVVRGFSFFGVSFVYVIFEDGTD
                     IYWARSRVLEFLNGAASRLPAGVTPTMGPDATGVGWVYQYAVMSKELNLSDTRTIQDW
                     NLKFALAKAEGVAEVASVGGFVRQYNVILDPQRMRDLGITMPKMRDAIRASNADVGGR
                     TVELSEFEYVIRGKGYLKGINDLGNIVLKTSNGTPVLLRDIARVELGPDERRGITELN
                     GEGEVASGIVLQRFGVNALDVIENVKKRFREIASSLPGSVEIVAVYDRSNLINAAIET
                     LKHTLFEESLVVALVCIIFLLHVRSALVAIIMLPVGILMAFGAMKLLGIGANIMSLGG
                     IAIAVGAMIDAAIVMIENAHKHLERADPGRSRVDVLIEAASEVGPALFFSLLIITVSF
                     MPIFTLESQEGRLFSPLAFTKTFSMAAAALLSVTLVPALMVVCVRGRIVPEHRNPINR
                     FLIWIYRPVIAGVMRAKVLVILVALAVLAVTIWPARQLGTEFMPNLNEGTLLYMPTTL
                     PGISVTKAGELMQTQDKIIRSFQEVASVYGKAGRASTATDPAPSEMFETIVNLKPKQE
                     WRPGVTIESLTAEMDRALQFPGVSNAWTMPIRARIDMLSTGIRTPVGVKVIGADLVEI
                     DRLAKQIEQVLKTVPGTSSAYAERGLGGYYLDVTPDRAALARYGIMVQDVQDVIASAL
                     GGQTVTTTVEGRQRFSVNMRYPRDLRSNAQAIASDVLVPMPNGGAVPLGEVAKIAPSR
                     GPTSIRTENGQLAVYIYVDIRDRDLGGYVAGAQHAVQASIAFPAGSYVVWSGQFEYLE
                     RAAARLKIVVPVTLSIIFLLLYLNFRSFADTMIVMLSLPFALVGGIWLMWWLGFNLSV
                     AVVVGFIALAGVAAETGVIMLIYLNHALAEIQAHRDADGSRLSRQDLYDAIMKGAVER
                     VRPKMMTVVAIMAGLLPIMWNTGTGSEIMQRIAVPMIGGMISSTLLTLIVIPAIYGIV
                     KGFRLPPEDRLDSQPPSLPPKSIKRPQAVSEPAE"
     gene            6594..7226
                     /locus_tag="VGA15_23825"
     CDS             6594..7226
                     /locus_tag="VGA15_23825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX9590756.1"
                     /translation="MAASDWRLEGEWIKNCNCAFGCPCDFNAPPTHGSCKGILGMRIT
                     KGHFEGTKLDGLVFMVTVDFPGPLHEGNGKIQPIIDERATPEQRQALFNIMSGKNSAE
                     GTLFQILSLIVAKIHDPVFVPFEFSFDKNGRVARVVAKGVLETEVEPIKNPVTGAPHR
                     IQVVMPDGFEHRSAEIASANIHSTGAIKFDTKGTHSSLANVVQTPDGVAA"
     gene            7282..8058
                     /locus_tag="VGA15_23830"
     CDS             7282..8058
                     /locus_tag="VGA15_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX9590757.1"
                     /translation="MNEMSALEYLLRRDRLIVAVGLAAVVALAWIYLATGAGMDTSMA
                     DMPMDMPMPWSPLSAALVFVMWWVMMIAMMVPSAAPTVLLFTTIRRKQEASVRPSEEA
                     WIFLGGYLLIWAGFSLIAALAQWGLERAGLLSMAMASTNAMLGGVILLAAGLYQFTPV
                     KNACLRYCQSPVLFLSRYWRPGAGGALRMGLRHGSYCLGCCWFLMALLFVSGIMNLVW
                     IPGIALYVACEKLLPIGRGLSRAAGVALIVSGAIVLARAS"
     gene            8142..8756
                     /locus_tag="VGA15_23835"
     CDS             8142..8756
                     /locus_tag="VGA15_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9590758.1"
                     /translation="MGFYSDYILPRLCDFAMRNEQLVPFRERVIGAAEGRVLEIGVGS
                     GLNLPFYRPLVREVLALEPASRLADMARSASGGASMPVSFLEASAEAIPLDEDCVDTI
                     VTTWTLCSIPEAGTALAEMRRVLRPGGKLLFAEHGLAPDEGVRRWQDRLTPAWRCISG
                     GCHLNRPIRSMIEGSGFRIDRIETGYIPGPKPMTFMYEGSARPN"
     gene            complement(8808..10112)
                     /locus_tag="VGA15_23840"
     CDS             complement(8808..10112)
                     /locus_tag="VGA15_23840"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX9590759.1"
                     /translation="MRPLVDGIPAYRRIAEAVVAARHSVWLTAAFVAPDFHMPDGSGS
                     LFDVLDRAVGRGLDVRVIFWRPNQESIGYGRTFSGSSDERGILKARGSRFRIRWDRAF
                     GPYCQHQKSWLIDAGQAGETAFVGGMNLTARAMGSPGHTAEGERHDAYVEIAGPSATD
                     VHHNFVQRWNEASERAADDGTWGHDGDDGLSFPARPSVPRGESVVQIQRTVHRGRYSD
                     AHASPESRAYDIACGERSVLAQYIQAIDAAHRSIYIENQAIPIPEVAAALEKALKRGV
                     EVVALVPADPEGAVRLARQNPQRKPLFDGVAALGQYENFALVGIAGLDARGCRSNIYV
                     HSKIMLVDDGWATIGSCNLHAFSLTGNTEMNAAIWDPKVVRALRCELLAEHLDRDTTG
                     IDDRAALRLYGEIARENRRRRDAGDCKWQGLAFSLDPATYGG"
     gene            complement(10227..>10510)
                     /locus_tag="VGA15_23845"
     CDS             complement(10227..>10510)
                     /locus_tag="VGA15_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipo-like protein"
                     /protein_id="HEX9590760.1"
                     /translation="GFDYTALHWADKQKPLQEVAGEFGLFPETISPPIVPEEIDEEAP
                     LPAEEVILGAGPTTLVERVIVSEHFIAVEYVAVHPPERRAHPREPEIAV"
BASE COUNT         1944 a         3248 c         3314 g         2004 t
ORIGIN      
        1 ggtggccgag caaggcgacg tatcccgcgg ccagcaggat ttccgggctt gtgcgccgtg
       61 ccactcgctc gaacccgacc gaaacatgac gggaccgagt ctggccaacc tctggggacg
      121 gaaggccggc ggactatcaa gcttcgagcg ctattccgaa gcgctcaagg cgtcgggaat
      181 catctgggat gatcggtcgc tggacggatg gctgaccgac ccggaccaca tggtcccgga
      241 caatgagatg cccttcaacg gcatcaagga tactcgcgct cgcgccgacc tgctggcgtt
      301 cctcagagac gcgaccaagc ccggagcgcc gcaacagacg gtgcaacagg gtgggatgaa
      361 gggcatggga ggaatgatgg gtgggggaat gatgggcggc ggaggcgacc ccaacctgaa
      421 agacctggaa cccgccaagc aggtcaaggc aatcacccac tgtcgcgata cctatcgcgt
      481 cacgacggca gacggcaaga cacgcgcttt ctgggaacgc aatctgcgct ttatgaccga
      541 ctcaagcaag gacgggccgg agagagaagt ccccgcgatc atgccggccg gcatgatggg
      601 cgaccgtgcg gccgtgattt tcgcagcacc cgaggaaatc gccaggatga tcgaagctcg
      661 atgctgagcc ctcgcgccga tgatagaacg acagcccggc acgatcgtag acatgtaccg
      721 gcgcggcgaa acacagcccc ggccgcgctg agagggtaac agtaactgga attttcaacg
      781 ccatgcagcg gttgtaacct ttcatttgcc gccattttgt gggactctgg gttttatgaa
      841 gcgcaacctc gccatacgcc ggctgctcgc gaccctggtg gtcgcgggcc ttgcgctggc
      901 tccggtttcc cggcccgtca tggcggagac ggcgtccgac gcgtcgatgc aggcagtggc
      961 ggatgagatg gcaccgtcat ccgcgacgac ggacgagatg gcgggcgaca tgccgtgctg
     1021 tccgtcaaag gcgccctcgc cggttgggtg cgataagtgc gtgtacatga cggcctgcgt
     1081 gtccaagtgc tttaccggca tgtcgtcgat agtcttgcat ccgttcctca ccgcttcgaa
     1141 tgatatcgct tctctgcaga acgatgcatg gcccgatggc ctcggccacc cgcctcccga
     1201 acatccgcct cgaattctgg tttgaacggc gtcaccgcgc cgtttgctgc cgcgcgactt
     1261 gatcgcgcgg gatcgacgca tgccgccttg cggcaagacc atgacatcag aggattctca
     1321 acatgaagac gttcaaactc tcgcgcgccc tccgggccgc gcttatcggc attgctatga
     1381 tcggttcggc cggcatcgcc cgcgccgaca tcaaggacta tgagttccag cttatcgacc
     1441 aaaccgtaaa ggccggatcg gacaagacca tcacggtacg gcttgtcaac aaagccaccg
     1501 gcaagtccgt tccggacgcc gtcatcttcg caacccgcct cgacatggcg ccggacggga
     1561 tgcaggaaat ggcaacaaaa attgcgccga ttaccagcgc tgagcctggc acttacaagt
     1621 tcaaggcaac attcggaatg gcgggccgct ggcagctttc gcttggcgca aaagtgcaag
     1681 gcgaaaccgg cacggtcgaa agcaagctcg tcatcacggc gcaaaagtga aacgcgccat
     1741 cgccggtgtc accgtcgccg ctttgatagc ggcggcgggt ggcggattca taggtgttcg
     1801 ccggcacctt catttgaccg cggcgccgct ggtttcgacg gccttggcgc agaccagcga
     1861 cgatccgatc tactatcagg atccggacgg caagccgttc tattcgctga cacccaagaa
     1921 ggtcccggac gggcgggatt accgtgccgt gcctgccggc gcggacctca gcttcgacga
     1981 cgaacccgcc gccgcacccg cgacagccgc ggcgaccgac cgcaagatca agttctatcg
     2041 caatccgatg gggctgccgg acacctcgcc aaccccgaaa aaggattcga tggggatgga
     2101 ctacatcccg gtctacgaag gcgaagatac cgacgatggt tccgtcaagc tttctcccgg
     2161 aaagatccag cggacgggtg tcaagtcgga gcccgcaagc cagcggataa tccgtaccgc
     2221 cattcgagca ccggggacca ttcaactcga cgaacgccgc gcatcgatcg tgtcgtttcg
     2281 gttcgagggc ttcatcgaaa gggtagaaaa cgtcaccacc ggcgaacacg ttcacaaagg
     2341 tcaggcgttg atgcgggtct atagcccgag tctatccagc gccgcggccg aatatttatc
     2401 ggcattgaac gcgaaagcag atagcggggt tagtgcccag gccctgaagg gcgcaagacg
     2461 gcggctggaa aatctcggca tgccggagaa ggccatcgcc gagatcgagc gcacgcgcga
     2521 aatctcgctg tccattccat ggctcgcgcc gcaggacggt gagatactgg agcgcgcggc
     2581 cgtgaacggc atgcgcgccg cgccgggcga catgctgttc cgcattgccg atcatcaggt
     2641 cgtgtgggtt ctggtcgata tcgccgagcg cgatctggag ggaattgcca tcgggcaaaa
     2701 ggtcgatgtg cgcccgcgcg cctttgcaaa ccgtgttttc agtggagatg tcgcgctgat
     2761 ctatccgcat ctcaacgctc agacgcgcac cgcgcgcgtc cgcattgagc ttgcaaatcc
     2821 ggatggcctg ttgcgccccg acatgtatgt ggacgcggag atcgacaccg gcagcccgca
     2881 gccggtgctg gcggttccgg agagcgccat tctcgatacc ggcagccgcc aggcggtatt
     2941 cgtcgacaag gggcagggcc gtttcgagcc gcgcgacgtg aaactcggcc atcgcggcgg
     3001 cggctatgtc gagattcgcc agggcgtcac cgagggcgaa ccggtcgtcg tctccgccaa
     3061 tttcctgatc gacgccgaaa gcaatttaat ggccgccctc aagggcttct cggacgcggg
     3121 agcccagcca tgatcgcccg cctcatcgcc tggtcggcgc gcaatttgtt gctggtgttc
     3181 tttggtgccg gttttgccgc cgccgccggg gtctacgcgc tcgcgcatct tcccctcgac
     3241 gccatcccgg atctctccga cacccaggtc atcgtctaca ccgaatatcc gggacaagcg
     3301 ccccaggtga tcgaggatca ggtcacctat cccttgacga cggccatgct caccgtgcca
     3361 cgatccaagg tggtgcgggg gttttccttc ttcggcgttt cgttcgtcta cgtgatcttc
     3421 gaggatggca ctgacatcta ctgggcccgc tcgcgggtgc ttgaattcct caacggcgcg
     3481 gcatcgcgcc tccccgcagg tgtgacgccg accatgggtc cggacgccac cggcgtcggt
     3541 tgggtctacc aatacgcggt gatgtcgaaa gagttgaacc tctcggacac ccgcaccatc
     3601 caggactgga acctgaaatt cgcgctcgcc aaggcggagg gcgtggccga ggtggcgagc
     3661 gttggtggct tcgtccgcca gtacaacgtc attctcgacc cgcagcggat gcgcgatctc
     3721 ggcatcacca tgccgaagat gcgcgacgcg atccgcgcca gtaatgccga cgtcggcggc
     3781 cgcaccgtcg aactctccga gttcgaatat gtcatccgcg gcaagggcta cctgaaaggc
     3841 atcaacgacc tcggcaacat cgtgctgaag accagcaatg gcacgccggt gctgctgcgc
     3901 gacatcgcgc gcgtcgagct cggtccggat gagcggcgcg gcatcaccga attgaacggc
     3961 gagggtgagg tcgccagcgg catcgtgctg cagcgtttcg gcgtcaacgc gctcgacgtg
     4021 attgagaacg tgaaaaaacg ttttagggaa atcgccagca gtctgccggg ctcggtcgaa
     4081 atcgtcgcgg tctacgatcg ctccaatcta atcaacgcgg cgatcgagac cctcaagcac
     4141 acgctgttcg aggaaagcct ggtggtggcg ctggtctgca tcatcttcct gctgcacgtc
     4201 cgcagcgcgc tggtggcgat catcatgttg ccggtcggca tcttgatggc gttcggagcc
     4261 atgaagctat tgggtatagg cgccaacatc atgagccttg gcggcatcgc cattgccgtc
     4321 ggcgccatga tcgacgccgc catcgtgatg atcgaaaatg cccataaaca tcttgaacgc
     4381 gccgaccctg gacggtcgcg ggtcgatgtc ctgatcgagg cggcttccga agtcgggccg
     4441 gcgctgtttt tcagcctgct gatcatcacc gtctcgttca tgccgatctt caccctggaa
     4501 tcgcaggagg gacggctgtt cagcccgctt gccttcacca agaccttttc gatggcggcg
     4561 gcggccttgt tgtcggtgac gctggtgccg gcgctgatgg tggtctgcgt ccgcggccgg
     4621 atcgtcccgg agcacaggaa cccgatcaac cgtttcctga tctggatcta tcggccggtg
     4681 atcgccggtg tgatgcgcgc caaggtgctg gtgatcctgg tcgcgctggc tgttctcgcg
     4741 gtcacgatct ggcccgcgcg ccagctcggc accgagttca tgccgaacct caatgaaggt
     4801 acgctgctct acatgccgac gacgttacct ggcatctccg tcaccaaggc cggcgaattg
     4861 atgcagaccc aggacaagat catccggtcg ttccaggagg tggcctcggt gtacggcaag
     4921 gctggtcgtg cttccaccgc caccgatcca gcaccttcgg agatgttcga gaccatcgtc
     4981 aatctgaagc cgaagcagga gtggcggccg ggcgtcacga tcgaaagtct gaccgccgaa
     5041 atggaccgtg ccttgcaatt cccgggcgtt tccaacgcct ggacgatgcc gatcagggcg
     5101 cgcatcgaca tgttgtcgac cgggattcgc acgccggtcg gggtcaaggt gatcggcgcg
     5161 gacctcgtcg agatcgacag actggcgaaa cagatcgagc aggttctcaa aaccgtgccg
     5221 ggaacgtcct cggcctacgc ggagcgcggg ctcggcggtt attatctcga tgtgacgccg
     5281 gaccgcgcgg cattggcccg ctatggcatc atggttcagg atgtccagga cgtcatcgct
     5341 agcgcgctcg gcggccagac cgtgaccacc actgttgagg gccgccagcg cttcagcgtc
     5401 aacatgcgct atccccgtga tctcagaagc aacgcgcaag ccatcgccag cgatgtcctg
     5461 gtcccgatgc ccaatggcgg ggcagtgccg ttgggagagg tggcgaagat cgcgccgtcg
     5521 cgcggcccaa catcgatccg caccgaaaac ggtcagctcg ccgtctacat ctatgtcgac
     5581 atccgcgatc gcgatctcgg cggttacgtc gccggcgccc aacatgcggt gcaggccagc
     5641 attgcgttcc cggcaggctc ctacgtcgtc tggagcggac agttcgaata tcttgagcgt
     5701 gcggcggcgc gcctgaagat cgtggtaccg gtcacgctct cgatcatctt tctgctgctg
     5761 tatctcaact tccgctcgtt cgccgatacg atgatcgtga tgctctcgct gcccttcgcg
     5821 ctggttggcg ggatatggct gatgtggtgg ctcggcttca acctttcggt cgcggtcgtc
     5881 gtcggcttca tcgcgctggc cggcgtcgcc gccgagaccg gtgtgatcat gctgatctat
     5941 ctcaaccacg cgctggcgga aatccaggcc caccgcgatg ccgacggcag ccggttgagc
     6001 cggcaggatc tgtatgatgc gatcatgaag ggcgcggtcg agcgggtgcg tcccaagatg
     6061 atgacggtgg ttgccatcat ggcgggcctg ctgccgatca tgtggaacac cgggaccggc
     6121 tccgaaatca tgcagcggat cgcggtgccg atgatcggcg gcatgatctc atcgacgctg
     6181 ctgacgctga tcgtgatccc ggcgatctat gggatagtca aagggttccg tttgccgccc
     6241 gaagaccgcc tcgacagcca gcctccttca ttgccgccca agtcgatcaa gcgacctcaa
     6301 gctgtttcgg agccagccga atgaggtgac cgatgatgcc cggaaccttc tggacgcaag
     6361 gacgcgggtg cgcaaggata tcctgttacc atctatggcc cgcctgtacg ctgagggcgt
     6421 tggctggtat gcttctatga tcaggatagc aaatcgcagt ccgctaaccg tctcaatcgt
     6481 ccgcggagca atgcagattc tgctcgaccc cggcttctca gcgctgaaga ggataatcag
     6541 gcggcgactg catagtccag ctgtcaacct gacaacgcga tgggaggtgt accatggctg
     6601 catctgactg gcgtctcgaa ggcgaatgga tcaagaactg caattgcgcg tttggttgcc
     6661 cctgcgattt caatgccccg ccgacccacg gctcgtgcaa gggcatcctc ggcatgagga
     6721 ttaccaaggg acacttcgag ggtaccaagc tcgatggtct cgtgttcatg gtaactgtcg
     6781 atttcccggg accgctgcac gagggcaatg ggaagattca gccgatcatc gatgagcgcg
     6841 ccacaccgga acagcgacag gcgcttttca acatcatgtc gggcaagaat tcggccgagg
     6901 gtacgctgtt ccagatcctg agcctgatcg tagccaagat ccatgatccg gtattcgtgc
     6961 ccttcgagtt ttcattcgac aagaacgggc gcgtcgcgcg tgttgtcgcc aagggcgtgc
     7021 tggagaccga ggtcgagccg atcaagaatc cggtgacggg cgctccccat cgcattcaag
     7081 ttgtgatgcc cgatggcttt gagcacagat ccgccgagat tgcctccgcc aacatccact
     7141 cgaccggggc aatcaagttc gacacaaaag gcacgcacag ctcgctcgcc aacgtcgtac
     7201 agacgcctga tggcgttgcg gcttgatgct gcgctaaggt tcgctctttc gacttgtcgc
     7261 gtcggcggcc gaggtgggcc catgaacgag atgtccgccc tcgaatacct gctgcggcgc
     7321 gaccggctga tcgttgccgt ggggctggct gcggtggtgg cgctggcgtg gatatatctg
     7381 gcgaccgggg caggcatgga cacgagcatg gccgacatgc cgatggatat gccgatgccg
     7441 tggtcgccgt tgtcagccgc gctcgtcttc gtcatgtggt gggtgatgat gatcgccatg
     7501 atggtgccga gtgctgcccc cacggtcctg ttgtttacga caatccggcg caagcaagag
     7561 gcctcggttc gtccttccga ggaagcctgg atctttctcg gtggctatct cctgatatgg
     7621 gcaggtttca gcctcatcgc ggccttggca cagtgggggc tcgaacgcgc cgggctgctg
     7681 tcaatggcga tggcgagcac caacgccatg cttggcggcg tcatcctgct tgccgcaggg
     7741 ctctaccagt tcacgcctgt caagaacgcc tgcctgcgct actgccagag cccggtgctg
     7801 ttcctgagca ggtactggcg acctggcgcg gggggagcat tgcgcatggg gctccggcac
     7861 ggcagctact gcctcggctg ttgctggttc ctgatggcgc tgctgttcgt cagcgggatc
     7921 atgaaccttg tctggattcc cggcatcgcc ctttatgtcg catgcgagaa gttgctgccg
     7981 atcggccgcg ggctcagccg cgccgctggt gtggccctga tcgtatcagg cgcgattgtt
     8041 cttgcgcgcg caagttgaga acaaacgcgc ccggcaatgc gaagccaccg tcaacgtgtt
     8101 cgcgacgtcg tcgcgttgag aggaacattc aggagcggtc gatgggcttt tatagcgact
     8161 acatcctgcc gaggctttgc gatttcgcga tgcgcaatga gcagctcgtg ccgtttcgcg
     8221 aacgcgtgat cggtgcggcg gagggcaggg tgcttgagat tggcgtgggc tccgggctga
     8281 acctgccgtt ctatcgcccg ctcgtgcggg aggtgctggc gctcgagccg gcatcgcggc
     8341 tggcggatat ggctcgaagc gcttccggtg gagccagcat gccagtcagc ttcctcgagg
     8401 cttccgccga ggccattccg cttgacgaag attgcgtcga taccatcgtc acgacctgga
     8461 ccctatgctc gatcccggag gccggcaccg cgctcgccga gatgcggcgc gtattgcggc
     8521 cgggcggcaa gctgctgttc gccgagcacg gtctggctcc cgacgagggc gtgcgccggt
     8581 ggcaggatcg gctgacaccg gcctggcgat gcatcagcgg gggctgtcac ctcaaccgtc
     8641 cgatccgttc catgatcgaa gggagtggct tccgtatcga ccgcatcgaa accggctata
     8701 tccccggacc gaagccgatg acattcatgt atgaaggcag cgcacggccg aactgacgag
     8761 acggatgcgg tacacttagt gccggtatgc cggtatattg cgcgtggcta tccgccgtat
     8821 gtcgcggggt ccaggctgaa ggccagaccc tgccatttgc aatccccggc atcgcgcctg
     8881 cggcggttct cacgcgcgat ttctccgtaa aggcggagtg ctgcgcggtc gtcgatgccg
     8941 gtcgtgtccc gatcgagatg ctccgccagc aactcacaac gcagcgcgcg caccaccttc
     9001 gggtcccaga tcgcggcgtt catctcggta ttgccggtga gcgaaaatgc atgcagattg
     9061 caggagccga tggtcgccca tccgtcgtct accagcatga tcttgctatg gacatagatg
     9121 ttgctgcgac acccgcgcgc gtccaggccg gcaatcccga ccagcgcaaa gttttcgtat
     9181 tggccgagcg cggccacgcc atcgaacaga ggcttacgtt gcgggttttg acgggccagg
     9241 cggacagcgc cttccggatc ggcgggaacc agagccacga cctccacgcc gcgcttgagg
     9301 gctttttcaa gagccgctgc gacttccgga atcgggatgg cctggttctc gatatagatc
     9361 gagcggtggg cggcatcgat cgcctggatg tactgcgcaa gaaccgaccg ttccccgcag
     9421 gcaatgtcgt aagcccggct ctccggactt gcgtgggcgt cgctatagcg gccccgatgc
     9481 accgtgcgct ggatctgcac gacactctcg ccgcgcggaa ccgacgggcg cgccggaaag
     9541 ctcaggccgt catccccgtc gtggccccat gtgccgtcgt cagccgcgcg ttcgcttgct
     9601 tcgttccagc gctggacgaa attgtgatgc acgtcggttg ccgaaggccc cgcgatctcg
     9661 acgtaggcgt catgacgttc gccctcagcc gtgtgaccgg gcgatcccat ggcccgggcg
     9721 gtcagattca tgcctccgac gaacgccgtc tcgccagctt ggccggcgtc gatcagccag
     9781 ctcttttgat gctgacagta aggcccaaac gcgcggtccc agcggatgcg gaaccgtgaa
     9841 ccccgcgcct tgagtatacc gcgctcgtct gatgatcccg agaaagtgcg gccgtagccg
     9901 atgctctcct gattcggacg ccagaagata acgcgcacgt ccaaacctcg cccgactgcg
     9961 cgatcgagca catcgaacag cgaaccactg ccatccggca tgtgaaagtc cggcgcaacg
    10021 aaggcggccg tgagccagac gctgtgccgc gctgccacga cggcttccgc gatgcggcga
    10081 taggccggaa tgccgtccac gagcgggcgc acggcatttc cggcgcgcgg gggatacgcg
    10141 cacgaggcca cgaaaggaat gtgggtattc ccggtcatgg ccagggttgt agcgtcaaat
    10201 atcggcgggt ggaattcgcg aaaaccctaa accgctatct ctggctcgcg cgggtgggcg
    10261 cgacgctccg gcggatgcac cgcaacgtat tcgacggcga tgaaatgctc tgacacgatg
    10321 acgcgctcga ccagcgtcgt cggcccggcg cccaatatta cttcttcagc cggaagcggc
    10381 gcttcttcgt caatctcttc aggaacaatt ggcggcgaga tcgtttctgg aaacaggccg
    10441 aattcgcctg ctacctcctg gagcggcttt tgcttgtcgg cccagtgcaa agccgtgtag
    10501 tcgaagcctt
//
LOCUS       DASWGM010000877         8547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1066190,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000877 DASWGM010000000
VERSION     DASWGM010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066190"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VGA15_23850"
     CDS             <1..1221
                     /locus_tag="VGA15_23850"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX9590761.1"
                     /translation="GVVTSLGAGKTDNWKKLTAGESGIRTITRFPTDGLKTTMAGTVD
                     FVPVEPFSSTDLSERLADIATEEAIAQSAIGSKGDFPGPLFLAVAPVEVEWPQRLELG
                     RAIGATVDYDGMLRVSGGGRFTQYHRRFLFGSVADHLAETFGTKGSPISLSTACASGA
                     SAIQLGVEAIRRGEADAALCVATDGSVNPEALVRFSLLSALSTQNEPPHAAVRPFSKN
                     RDGFVMAEGAGALVLESYEAAAARGAKILGIVAGCGELADSFHRTRSSPDGKPIIGCV
                     RNALSDAGIVADQIDYINAHGTGTPENDKMEYLGISTVFGERTSQVPVSSNKSMVGHT
                     LSAAGAVEAVFSLLTLEHQRIPPTINYDIPDPAIPFDVVPNTARDAHVTAVMSNSFGF
                     GGQNAVLILTREPV"
     gene            1416..1535
                     /locus_tag="VGA15_23855"
                     /pseudo
     CDS             1416..1535
                     /locus_tag="VGA15_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
     gene            1535..2467
                     /locus_tag="VGA15_23860"
     CDS             1535..2467
                     /locus_tag="VGA15_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEX9590762.1"
                     /translation="MHPVLLRTKARIRDATKPLGEAAVGALTIGMLRATRYFDPIKTA
                     NLFGRILRLVGPMTREQKIGRANLTAAFPEKSPAEIEVILAGVWDNLGRLGAEFAHLD
                     HIWEHDPAHPEDSRIEIQTRTHELFAQLRLDGKPALIFTSHLGNWELPALAAVAHGLD
                     AAILYRRPNIAAANRIIEEIRAVKMGTLIPAGRDAPLKLADALQNGQHVAMLVDQYFT
                     NGVDVTFFGRKTKANPTLARLLRQIECPVHGVRIIRLPGHRFRAELSEEVQPVRDAAG
                     KIDVQGTMQAVTSVIEGWIREYPDQWLWLHRRWR"
     gene            complement(2661..3779)
                     /locus_tag="VGA15_23865"
     CDS             complement(2661..3779)
                     /locus_tag="VGA15_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9590763.1"
                     /translation="MIYRRCRPRIPAVVAIAAVVVALVAPSARAEERTVNFYNWSNYM
                     APGVLEDFTRETGIKVVYDTFDANETLETRLLAGKSGYDVVVPTAYFLQRQITANVFQ
                     KLDKAKLPNLANAWGVVTKRLATYDPGNLYAANYMWGTTGIGYNVKAVQKILGPDAKI
                     DSWDIVFKPENLEKFRDCGIHMLDSADDIFAAALGYLGLDPNSTKQADLERAADLVIK
                     IRPYVRKFHSSEYLSALATGEICFVVGWSGDIMQARSRAAEAKSDIEIGYAIPKEGAQ
                     MFFDNLAIPADAKNVAEAYELINYLYRPEVAAKNSDFLSYANGNLASQKLVDPKILND
                     KNIYPDEATLAKLFVITARDPATQRVINRLWTRVKTGR"
     gene            complement(3790..4953)
                     /locus_tag="VGA15_23870"
     CDS             complement(3790..4953)
                     /locus_tag="VGA15_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX9590764.1"
                     /translation="MTSMNKVFADLPVTIFEAMSQLARDNDAINLGQGFPDDPGPEDI
                     RRAAADAVMDGYNQYPSMMGIPELRQAIAAHYGRWHGLTLDPMTEVMVTSGGTEALSS
                     AILAVVEPGDEVVVFQPVYDSYLPIIRQAGGIPRLVRLEPPQWRLTEEALRGVFNHNT
                     KAVLFNNPLNPAAVVYPREDLELLARFCQEFDTVAICDEVWEHVIFDGREHIPLITIP
                     GMRDRTIKVGSAGKIFSLTGWKIGFVCAAPPLLRVAAKVHQFLTFTTAPNLQVAVAYG
                     LGKPADYFYQMRKELARSRDRLTQGLERIGFPVLRSQGTYFLTVDLSPLGLNETDEAF
                     CKRIVTDYKVAAIPVSAFYETDAVTSVVRFCFAKKDKTLDTALERLSDAVHRR"
     gene            complement(4961..5293)
                     /locus_tag="VGA15_23875"
     CDS             complement(4961..5293)
                     /locus_tag="VGA15_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX9590765.1"
                     /translation="MADQGRVHDLTPEQVSKGMAEGRYLLVDVREPNEVAAEAYPGAV
                     VVPLSSFDPEDIPDPQGKQVVFACRSGKRSVTASLAAQAGGLAYDKHLAGGMLAWKAA
                     GLPTKTGG"
     gene            complement(5522..7447)
                     /locus_tag="VGA15_23880"
     CDS             complement(5522..7447)
                     /locus_tag="VGA15_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX9590766.1"
                     /translation="MTSDVVVPAVEAAAANGHGDAHSTAGFKALTLGSIGVVYGDIGT
                     SPLYALREAVVAASGPSGIVSPQAVLGVVSLILWALIIVVTLKYVVILLRADNNGEGG
                     TLALMALAQRAVSSGAGNIVLLGIIGGALFYGDAVITPAVSVLSAIEGMTIVTAAFEP
                     YVVALTVVILLALFAVQSRGTARVAAFFGPIMCVWFAVIAVAALPQIARHPEVLLAFS
                     PGYAVFFMFHHGIIGFVTLGAVFLAVTGAEALYADLGHFGKRPIQTAWLFIVLPSLAL
                     NYLGQGALVLGNPKTLENPFFLMFPDWALFPMVVLAIMAAVIASQAVITGAYSLTRQA
                     IQLGLMPRFEIRHTSESHSGQIYIPRVNMLLSIGVVLLIVLFRSSSALASAYGIAVTG
                     TMVVTAMMGFVVIWKVWKWSPFAAAALIAPFLFLDLIFLSANLLKVFEGGWVPLALGA
                     VVMLLMYTWRRGSRLLFEKSRKLEFPLADLVSMLEKRPPQRVSGTAVFLTSDPVSAPT
                     ALMHSLKHYKVLHEKNVILTIETAPTPRIDPAERVRLEQISATFSKVTLRFGFMESPN
                     VPKALAIARKLGWHFDIMSTSFFLSRRALKPAAHSGMPRWQDHLFISLSRTANDATDY
                     FQIPSGRVVEVGTQVTV"
     gene            complement(7594..>8547)
                     /locus_tag="VGA15_23885"
     CDS             complement(7594..>8547)
                     /locus_tag="VGA15_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX9590767.1"
                     /translation="TGAYSLTRQAVQLGLLPRFEVRYTSESHAGQIYLPRVNRLLLIG
                     VLLLVLLFRTSSGLASAYGIAVSTTMVADGIMGFVVIWKLWNWRAATPAALIVPFVVV
                     DMTFFSANLLKLLEGAWVPLLFGFAMAIMIWTWRRGAAILIAKTRRTEVPLKDLIKSL
                     EKRPPHVVKGTAVFLTSDPNFVPTALLHNLKHNKVLHEHNVILTIETAQTPRVDPGER
                     VRMETISDKFSTVRLRFGFMESPNVPKALVIARKLGWQFDIMATSFFVSRRSLKPSAQ
                     SGMPQWQDHLFIALSRSANDATDYFQIPTGRVVEVGTQVTI"
BASE COUNT         1741 a         2669 c         2549 g         1588 t
ORIGIN      
        1 ggtgtcgtga catcgctcgg cgccggcaag accgacaact ggaaaaaact caccgccggc
       61 gaatccggca ttcgaaccat tacgcgtttt ccgaccgacg gcctgaaaac gacgatggcc
      121 ggtacggtcg atttcgtgcc ggtcgagcct ttttcctcga ccgatctttc ggagcggctt
      181 gccgatatcg ccaccgaaga agccatcgcg cagtccgcga tcgggagcaa aggtgatttt
      241 cccggaccgc tgtttcttgc cgtcgcccct gtcgaggtcg agtggccgca gcgactggaa
      301 ctgggacgcg cgatcggcgc gacggtcgat tatgacggca tgcttcgcgt cagcggcggc
      361 ggaaggttca cgcaatatca ccggcgtttt ctgttcggtt cagtggcgga tcacctcgcc
      421 gagacattcg gcaccaaagg ttcgccgatt tcgctttcga cggcatgcgc atccggcgcc
      481 agcgccatcc agcttggcgt tgaagccatt cgccgcggcg aagccgacgc cgcgctgtgc
      541 gtcgcaactg atggctcggt caatccggaa gccctggtgc ggttctcatt gctgtcggcg
      601 ctatcgaccc aaaacgagcc gccccatgcg gcggtccggc cgttttcgaa gaaccgcgac
      661 ggcttcgtga tggcggaggg cgccggcgcg ctggtgctgg aaagctacga ggccgccgca
      721 gcgcgcggcg ccaagatcct tggaatcgtc gccggctgcg gagaactggc ggattcattc
      781 caccgcaccc gatcgagtcc cgacggcaag ccgatcatcg gatgcgtgcg caatgcattg
      841 tcggatgccg gcatcgtcgc cgaccagatc gactacatca acgcccacgg caccggaacg
      901 ccggagaacg acaaaatgga atatctgggc atctcgacgg tgttcggcga acgcaccagc
      961 caggttccgg tttcctccaa caagtcgatg gtcggccata ccctctccgc ggcgggcgcg
     1021 gtcgaagcgg tgttctcgct gctgacgctc gaacaccagc ggattccgcc gactatcaat
     1081 tacgatattc cggacccggc gatacccttc gacgtggttc ccaacaccgc gcgcgacgcc
     1141 cacgtcaccg cagtgatgtc gaattcgttc ggcttcggcg gccagaatgc tgtgctgatt
     1201 ctaacgcgcg aaccggttta gcggcttagt cccttcccat gtcggcggct gacgtcatcg
     1261 taccaaccgg cgacacgtac gcgatcatcc atccttcgtc ttgccggaga cacggcctca
     1321 tgcgtgcgct cactcttgtt gccgatcgtc agctggtgat tgcgcaagtg cccgcgccgc
     1381 cgcccccggg cgccggcgag gtgcagatcc gcgtcaagat ggccggcggc atgcatccgg
     1441 cgatcgacac cgaggtgcca atcgaggatg ttgccaccgc gctgaagcgc atggaaagcc
     1501 ggcaagtgtt cggcaagatc gtcgttgatt tctgatgcat cccgttctgc ttcgcaccaa
     1561 ggcccgtatc cgcgacgcga ccaagccgct gggcgaggcc gcggtgggcg cattgacgat
     1621 cggaatgctg cgcgccaccc gctatttcga tccgatcaag accgcgaacc tgttcggccg
     1681 gattctgcgt cttgtcggac cgatgacgcg cgagcaaaag atcggccgcg ccaatctcac
     1741 cgccgcgttc ccggaaaaat cgcctgcgga aatcgaggtc attctcgccg gcgtctggga
     1801 caatctcggc cgcctcggcg ccgaattcgc gcatctcgac catatctggg agcacgatcc
     1861 cgcgcacccc gaggatagcc ggatcgagat ccagacgcgc acgcacgaat tgtttgcgca
     1921 gctgcggctc gatggcaaac cggcgctgat ctttacaagc cacctcggca attgggagct
     1981 gcccgcgctg gctgccgtcg cgcatgggct ggatgccgcg atcctgtatc gccggcccaa
     2041 tatcgctgcc gcaaaccgca tcatcgaaga gatacgcgcg gtgaagatgg gaacgctgat
     2101 ccccgccggt cgtgacgccc cgctaaagct cgcggatgcc ctgcaaaatg gccagcacgt
     2161 cgccatgctg gtcgaccagt attttaccaa tggtgtcgac gtgacgtttt ttggccgcaa
     2221 gaccaaagcc aatccgacgc tggcgcggct gctgcggcag atcgagtgtc ctgttcacgg
     2281 cgtccgcatc atccgcctgc ccggccatcg ttttcgcgct gaactatccg aagaagtgca
     2341 gccggtacgc gacgccgccg ggaaaatcga tgtccaggga accatgcagg cggtgacctc
     2401 ggtgatcgag ggctggattc gggaatatcc cgatcagtgg ctatggctgc atcgccgctg
     2461 gcggtagctc tccagcttat gtgagcagtg cgctttcgtc tcgtcattgc gaggagccaa
     2521 cgggtcgcgc gaatgcgcgc ccgatgacag gctccgcgac gaagcaatcc atgccacgca
     2581 cgaaggtttg gattgcttcg cggagcctgt catcgggcgg cgctttgcgc cgacccgttg
     2641 gctcgcaatg acggagagac ctatctcccc gtcttgaccc gcgtccagag ccggttgatg
     2701 acgcgctggg tcgccggatc gcgcgcagta atcacgaaca gttttgcaag ggtcgcctcg
     2761 tccggataga tgttcttgtc attgaggatt ttcgggtcga ccagcttctg gctcgccaga
     2821 ttgccgttgg cgtaggatag aaaatccgaa ttctttgccg cgacctccgg ccggtagaga
     2881 tagttgatca actcataggc ctcggcgaca ttcttggcat cggccgggat tgcgagatta
     2941 tcgaaaaaca tctgcgcgcc ttcctttgga atggcgtagc cgatctcgat atcgcttttt
     3001 gcctcggcag ctcggcttcg cgcctgcatg atgtcgcccg accagccgac cacgaagcag
     3061 atttcccccg tcgccaacgc gctcagatat tccgatgaat gaaattttcg gacatagggc
     3121 ctgatcttga taacgagatc ggcggccctt tcgagatcgg cctgtttggt cgagttcgga
     3181 tcgagcccga gataaccgag cgccgcggca aaaatatcat cggcggaatc cagcatgtga
     3241 atgccgcagt ccctgaattt ttccaggttc tccggcttga acacaatatc ccagctgtcg
     3301 atcttggcat cgggaccgag gatcttttgc accgccttga cgttgtagcc gatcccggtg
     3361 gtgccccaca tgtagttggc ggcatagagg ttgccgggat cgtaggtggc cagccgcttc
     3421 gtcaccacgc cccacgcgtt ggcaaggttc ggcagttttg ccttgtcgag tttttggaaa
     3481 acattcgccg tgatttgccg ctgcaaaaaa taggcagtgg gaacgacgac atcgtaaccg
     3541 gattttcccg cgagcagccg cgtctccagg gtctcgttgg cgtcgaaggt gtcgtagacg
     3601 accttgatgc cggtttcccg agtgaagtct tcgagcaccc ccggcgccat atagttcgac
     3661 caattgtaga aattgacggt gcgctcttcc gccctcgcgg acggcgcaac cagtgcaacg
     3721 accaccgcgg caatcgcgac aacagccggg attcgaggac ggcaccgtcg gtatatcatt
     3781 cgctacctct tatcgccggt gcaccgcgtc cgacaggcgt tccagcgcgg tgtcgagcgt
     3841 cttgtctttc ttggcgaaac agaaacgcac caccgaggtc accgcatccg tctcataaaa
     3901 ggccgacacc gggatcgccg cgaccttgta gtcggtcacg atgcgcttgc agaacgcctc
     3961 gtcggtctcg ttgagaccga gcggcgaaag atcaacggta aggaaatagg tgccctgcga
     4021 ccgaagaacg ggaaagccga tgcgctccaa tccttgcgtg agacggtctc ggctgcgcgc
     4081 cagttccttg cgcatctgat aaaaataatc cgccggcttg ccgaggccat aggccaccgc
     4141 gacctgaaga ttgggcgcgg tggtgaaggt gagaaactgg tgcaccttcg cagcgacgcg
     4201 caacagcggc ggtgcggcgc agacgaagcc gatcttccag cccgtcagcg aaaaaatctt
     4261 gcctgctgaa cctaccttga tggtgcggtc gcgcattccc ggaattgtaa tcagcggaat
     4321 atgttcgcgg ccgtcgaaga tcacatgctc ccagacctcg tcgcagatcg caaccgtatc
     4381 gaactcctga cagaaccgcg ccagcaattc gaggtcttcg cgcggataga ccaccgcggc
     4441 cggattgagc ggattgttga acaggaccgc cttggtgttg tggttgaaaa cgccccgcag
     4501 cgcttcttcc gtcagccgcc actgcggcgg ctcgaggcgc accaggcgcg gtatgccccc
     4561 ggcctggcga atgatcggca gataggaatc gtacaccggc tggaaaacca ccacctcgtc
     4621 gccgggctcg accacggcaa ggatcgcgct gctcagcgct tcggtgccac cggacgtcac
     4681 catcacctcg gtcatcggat cgagggtgag gccatgccag cgcccgtaat gggccgcgat
     4741 cgcctggcgc agttccggaa tgcccatcat cgaggggtat tgattgtagc cgtccatgac
     4801 ggcatccgcc gccgcgcggc gaatgtcctc aggcccggga tcatcgggaa aaccctggcc
     4861 gagattgatg gcgtcgttgt cgcgcgcgag ctgcgacatc gcctcgaaga tggtcacggg
     4921 aagatccgca aagaccttgt tcatggaagt catggggaag tcagccgccg gtcttggtcg
     4981 gcagtcccgc ggccttccag gccagcatgc cccctgcgag atgtttgtcg taggccaacc
     5041 cgccggcttg cgcggccagc gacgccgtta ccgatctttt ccctgaccgg caggcaaaaa
     5101 ccacctgctt gccttgggga tcgggaatat cctccggatc gaaactcgaa agtggaacga
     5161 cgaccgcgcc gggatatgct tcggctgcga cctcgttcgg ctcgcggacg tcgacgagaa
     5221 ggtagcggcc ctcggccatt cccttcgata cctgttccgg cgtcaaatcg tgaacccgcc
     5281 cctgatccgc cactcaaatc ctcccagtct catctctttc agcatgatct ccgggcaaac
     5341 gcgtcgcgtt tgtcccgagg gaaagccggt tctgaccccc ggatcaagtc caagggcatg
     5401 cttttccgga tcatgttcca gcaagagcaa attcggcaat gccgcgcgct tgctcgccct
     5461 tgcgcgccaa cctcgcccct cgcgcggcga aaatcaagcg ccgggaaccg gcctcaaaac
     5521 ctcaaaccgt gacttgcgtc cccacctcga ccacccgacc gctcggaatc tgaaaatagt
     5581 cggtcgcatc gttggcggtt cggctgagcg atatgaagag gtgatcctgc cagcgcggca
     5641 ttcccgaatg cgccgccggc ttcagcgcac gccgcgagag gaagaacgac gtcgacatga
     5701 tgtcgaaatg ccaaccgagc tttcgcgcga tcgccagcgc tttcggcacg ttaggcgatt
     5761 ccatgaagcc aaaccgcagc gtgaccttgg agaaggtcgc gctgatctgt tccagccgga
     5821 cgcgctcggc ggggtcgatc cgcggcgtcg gcgccgtctc gatggtgaga atgacgttct
     5881 tctcgtgcag cactttgtag tgtttcagac tgtgcatcag ggcggtcgga gcgctgacgg
     5941 gatcgctggt caggaatacg gcggtgccgg aaacccgttg cggcggccgc ttctccagca
     6001 tcgacaccag atcggccagc ggaaactcca gcttgcgcga cttctcgaac aacagccggc
     6061 tgccgcgccg ccaagtgtac atcagcagca tcaccacggc tcccagcgcc agcggcaccc
     6121 agccgccctc gaataccttc aaaaggttcg ccgaaagaaa gatgagatcg aggaacagaa
     6181 atggcgcgat cagtgcagct gccgcgaacg gcgaccattt ccagactttc cagatcacga
     6241 caaaacccat catcgccgtg accaccatgg tcccggtgac cgcgatgccg taggccgatg
     6301 ccagtgcact cgatgaacgg aacaggacga tgagcagaac cacgccgatc gacagcagca
     6361 tattgacccg ggggatgtag atttggccag aatgggattc cgaggtgtgc ctgatttcaa
     6421 accgcggcat cagccccagt tgaatggcct ggcgggtcaa cgaataggcg ccggtaatca
     6481 ccgcctggct cgcgatcacg gcggccatga tggcaagtac gaccatcgga aacaacgccc
     6541 aatccggaaa catcaggaag aatggatttt cgagcgtctt cggatttccc aaaacgagcg
     6601 cgccctgtcc cagataattg agcgccagcg acggcagcac gatgaaaagc catgccgttt
     6661 gaatcggacg tttgccgaaa tgtccgaggt cggcgtagag cgcctccgcc ccagtcacgg
     6721 caaggaagac ggccccgagg gtaacaaatc cgatgatgcc gtgatggaac atgaaaaaga
     6781 cggcatagcc agggctaaag gccagcagca cttccggatg tcgtgcgatc tgcggcaagg
     6841 ccgccactgc gatcacggcg aaccacacac acatgatcgg tccgaaaaag gccgcgactc
     6901 gagccgtgcc acgcgactgc accgcaaaca gcgcgagtaa tatcaccacc gtcagcgcca
     6961 ccacgtacgg ttcaaacgcc gcagtgacta ttgtcattcc ttcgattgcc gaaagcacgg
     7021 agactgccgg cgtgatcacc gcatcgccat aaaaaagagc accaccaata attccgagaa
     7081 gaacgatgtt gccggcaccc gaactcaccg ctcgctgcgc gagcgccatc agcgccagtg
     7141 ttccgccctc accgttgttg tccgcgcgca gcaggatcac cacgtatttc agcgtgacga
     7201 cgatgatcaa cgcccacagg atcagggata cgacgccgag gaccgcctgc ggactgacga
     7261 tgcccgaggg cccgctggcc gcgaccacag cttcgcgcag tgcgtacaac gggctggtgc
     7321 cgatgtcgcc gtatacgaca ccgatgctgc cgagcgtgag ggccttgaag ccggcagtgg
     7381 aatgggcatc gccatgccca ttggccgccg cggcttccac ggcgggaact acaacatcgc
     7441 tagtcatggg ggcggaggcc tcaaaaatgc ttcgctgcac catagcaaag ggaggcggct
     7501 tatagccctg cgcatggagc atagcctacc ctgatttcgg gccctcgcca tgcaaaaatg
     7561 catgaattgt tggattgtac cttagattgt tactcagatt gtgacttggg tcccgacttc
     7621 aaccacgcgc ccggtcggaa tctgaaaata atcggtggcg tcgttcgccg accggctgag
     7681 cgcgatgaac aggtggtcct gccactgcgg cattccggac tgcgcggatg gcttcagcga
     7741 gcgccgcgag acgaaaaacg atgtcgccat gatatcgaac tgccagccga gcttgcgcgc
     7801 tatcaccagc gccttgggaa cgtttggcga ttccatgaaa ccgaagcgca gccggacggt
     7861 ggaaaatttg tcgctgatcg tttccatccg gacccgctcg ccgggatcga cgcgcggcgt
     7921 ctgcgcggtt tcgatggtca ggatcacatt gtgctcgtga agcaccttgt tgtgcttgag
     7981 attatgcagc agtgccgtgg gcacgaaatt cggatcgctg gtgagaaaaa ccgcggtgcc
     8041 tttgacgacg tgcggcggac gcttttcgag actcttgatc aggtccttta gcggaacttc
     8101 cgttcgccgg gtcttggcga tcaggatcgc agccccccgc cgccaggtcc agatcatgat
     8161 cgccatggca aacccgaaca gcagcggcac ccaggcgccc tcaagcagct tcaaaagatt
     8221 ggcgctgaag aacgtcatgt cgaccacgac gaacggaacg atcagcgccg ccggggtggc
     8281 cgcgcgccag ttccacagct tccagatcac gacgaaaccc atgatgccat cggccaccat
     8341 ggttgtggaa accgcgatgc cataggcgga ggccaatccg cttgaggtac ggaacagcag
     8401 gaccagcagc aagacgccga tcagcaacag ccggttcacg cgcggcaaat aaatctgtcc
     8461 ggcgtgcgac tcggaggtgt agcggacctc gaagcgcggc agcaggccaa gctgcacggc
     8521 ctgacgggtc agcgaatacg ctccggt
//
LOCUS       DASWGM010000878         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1904554,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000878 DASWGM010000000
VERSION     DASWGM010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904554"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGA15_23890"
     CDS             complement(<1..513)
                     /locus_tag="VGA15_23890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX9590768.1"
                     /translation="MIGRVGILLGIVLLGIQPARAADSGFTRFIASLWPEAQAAGVSR
                     ATFDAETRGLEPDYKLPDLILPGRPATGAPSQAEFVQVPADYIKEASIARLAEEGQRL
                     MAKHRYALSAIEARFGVPATVVLAIWGRETDYGRYTLPYDAVRVLATQAYVGRRKDQY
                     RTEFILALKII"
     gene            619..1077
                     /locus_tag="VGA15_23895"
     CDS             619..1077
                     /locus_tag="VGA15_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590769.1"
                     /translation="MFFPKTNVKNDPLRRWHRPDRHFFANGACQVLAFAFLERYPDMG
                     FHARWIKPAAGFTGNHIYVTDGINAFDYHGLTTEQRLLAFGFKRGRRFFPGWAATLVD
                     LPADVLVSEQGSRQIEGLWLREPKQFLHDALPRARNFLESFGDIRLRLAT"
     gene            complement(1095..1865)
                     /gene="tam"
                     /locus_tag="VGA15_23900"
     CDS             complement(1095..1865)
                     /gene="tam"
                     /locus_tag="VGA15_23900"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HEX9590770.1"
                     /translation="MADWSAAQYLKFEDERTRPSRDLLAQVPLAEARRVIDIGCGPGN
                     STELLVRRWPQAEVIGVDTSADMLRQARERLPGRTFTEANVVHWVPPEKTDVLFANAI
                     FQWVPGHLRQLQRLLGALTPGGVLAVQMPDNLDEPAHVLMREVALLEPWRTTLAETAR
                     LRDALPKPGVYYDALGPLCSRLEIWHTIYNHVLDDAAAIVEWVKGSGLRPFVDPLDPP
                     ERKAYLAEYTARIASSYLPQADGKVLLRFPRIFIVAVR"
BASE COUNT          372 a          676 c          598 g          378 t
ORIGIN      
        1 aatgatcttc agcgccagaa tgaactcggt gcggtactga tccttgcggc ggccgacata
       61 ggcttgcgtc gccagcaccc gcaccgcatc gtagggcagc gtgtagcggc catagtcggt
      121 ttcgcggccc cagatcgcca gtaccacggt ggcgggcacg ccgaaacgcg cttcgatcgc
      181 gctcaaggcg tagcgatgct tcgccatcag ccgttgcccc tcctccgcca gccgcgcaat
      241 gctggcttcc ttgatgtaat cagccggcac ctgcacgaac tcggcctgcg atggcgcgcc
      301 ggtcgccggc cgtcccggca ggatcaagtc gggcaatttg taatccggct caagaccgcg
      361 ggtctcggca tcaaaagtcg cgcgcgacac cccggcggcc tgcgcttccg gccacagcga
      421 ggcgatgaat cgggtgaagc ctgagtcggc ggcgcgggcg ggctgtatgc ccagaagaac
      481 gattcccaac agaatgccga cccgtccaat catggctgca tattgcactc acgcacctgt
      541 tccgtcacgt ttggcgaccg gctgcgcgga tgagtcgccc aacccacccg attgtaacca
      601 gtcgagatcg tcagcgcgat gttcttccca aagaccaacg tcaagaatga tccgctccgt
      661 cggtggcacc ggcccgaccg gcacttcttc gccaacggag cctgtcaggt gttggccttc
      721 gcgttcctcg aacgctatcc cgacatgggt ttccatgccc gctggatcaa accggcggcc
      781 gggtttaccg gcaaccacat ctacgttacc gacgggatca acgcattcga ttatcacggc
      841 ctcacgacgg agcaacggct gctcgcgttc ggcttcaagc gtggccggcg gttttttcca
      901 ggctgggcgg caacgcttgt ggacttgccg gccgacgttc tcgtttccga acagggctca
      961 aggcaaatcg aaggtctgtg gctgcgcgaa ccaaagcaat tccttcatga cgcgctgccc
     1021 cgcgcccgca actttctcga aagcttcggc gatatcagac ttcgacttgc cacgtgacgc
     1081 tgccgcctca gccctcacct caccgccaca atgaaaatcc gaggaaaccg cagcagcacc
     1141 ttgccgtcgg cctgcggcag atagctcgac gcgatgcgcg cggtgtattc ggcgagatat
     1201 gctttgcgtt cgggcggatc caggggatcg acgaacggcc gcaggccgct tcccttgacc
     1261 cactccacga tcgcggcggc atcgtcgagc acgtggttat agatcgtgtg ccaaatttcc
     1321 agccggctgc agagcgggcc cagcgcatcg taatagacgc ccggcttcgg aagagcatcg
     1381 cggagccgtg cggtctcggc cagcgttgtg cgccacggct ccagcagcgc gacctcgcgc
     1441 atcaggacat gggcgggctc atcgagatta tcgggcattt gcaccgcaag gacaccgccc
     1501 ggcgtcaacg cgccgagcag ccgctgcagt tgcctgagat ggccgggcac ccactggaag
     1561 atcgcattgg caaacagcac gtcggtcttt tccggcggca cccagtgcac gacgttggcc
     1621 tcggtaaacg tccgtcccgg cagccgttcg cgcgcctgcc gcagcatgtc ggccgaagta
     1681 tcgacgccga tcacctctgc ctgcggccag cgcctgacca gaagctcggt tgaattgccc
     1741 ggaccgcacc cgatatcgat gaccctgcgc gcctcggcaa gcgggacttg cgccaagagg
     1801 tcgcgagacg ggcgggtccg ctcatcctcg aacttgagat attgcgcagc gctccagtcc
     1861 gccatcgcca ggattccagt ttaccgcgtc agcttcttgt acttcatgcg atgcgggatg
     1921 atggaatcct gtcccagcct gcgcatcttg tccttttcat aatcctggaa attgccctcg
     1981 aaccattcga cgtggctgtc gtcctcgaag gccaggatat gcgt
//
LOCUS       DASWGM010000879         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_510256,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000879 DASWGM010000000
VERSION     DASWGM010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510256"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /gene="rlmJ"
                     /locus_tag="VGA15_23905"
     CDS             <1..534
                     /gene="rlmJ"
                     /locus_tag="VGA15_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX9590771.1"
                     /translation="ALLRPQDRLTACEVEPKARKRLIEALRRDTQARVVDLDGWVALP
                     AFVPPNERRGLVLIDPPYEQNDEFERIAAGFTEAFAKWPTGSYLLWYPVKSRRATDTL
                     ARHVAEVAGAGAPPGKCLRLEFSVAPQAAGLNSAGLLIVNPPWTLAGELKAILPELEK
                     PLGQGGAGRFRLETPKP"
     gene            872..1087
                     /locus_tag="VGA15_23910"
     CDS             872..1087
                     /locus_tag="VGA15_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX9590772.1"
                     /translation="MAMTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLI
                     EGQRITFEVEPDKKGKGPKAVNLVISA"
     gene            complement(1469..2131)
                     /locus_tag="VGA15_23915"
     CDS             complement(1469..2131)
                     /locus_tag="VGA15_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX9590773.1"
                     /translation="MSRLVLAAIALTGAGFAVSAQAADIPYYGSRPPPYAGPPPVVQP
                     FDCYSWAGPYLGGNLGYAWGSVDNNRTKPSGFVGGVQAGYNWQPSPSWVFGVEGDLQV
                     TGADETFAPWKFSNPWFSTVRGRAGYAFSNILVYGTAGLAFGELRGETFGLSETHANA
                     GWTAGVGAEFGFGQTGFSRGGWSAKVEYLYVDLSNSNFTITGVPNGYRFGTLRAGINY
                     HF"
     gene            2421..>4316
                     /gene="uvrC"
                     /locus_tag="VGA15_23920"
     CDS             2421..>4316
                     /gene="uvrC"
                     /locus_tag="VGA15_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX9590774.1"
                     /translation="MTSDSPDTPRAPRPPISQPGSRPDLPPDPSSGDIDPAMANAWED
                     DEVLLPDVPEEANEAVPEGPLAVGHAAIEHAVRHAPTSPGVYRMLNAASDVLYVGKAK
                     NVRKRLASYARVAAPQPARILRMIAATVTVEIISTSTETEALLLEANLIKQLRPRFNV
                     QLRDDKSFPYILITGDHWAPQILKHRGAQSRPGQYFGPFASAGAVNRTITALQRAFLV
                     RSCTDGFFESRTRPCLLYQIRRCSGPCTGEIDFPGYTELVREAKDFLSGRSRLVKQEL
                     AGEMEKASAELAFETAALYRDRLAALSAIQSQQGINPRTVEEADVFAIHQEGGYSCVE
                     VFFFRTGQNWGNRAYFPRAEKSFTPEEVLASFLAQFYDDKPPPKLILLSHPIEECELL
                     ADALSIKAGFKVEVSTPRRGEKKELIAHALTNAREALGRRLADTATQGRLLAGLVTTL
                     GLPHPPKRIEVYDNSHIQGTNAVGAMIVAGPDGFIKNQYRKFNIKSEGLTPGDDYAMM
                     REVLQRRFKRLLTPPVEGDAAKPKADDDSFPQWPDLVIIDGGRGQLNAVREIFDSLGL
                     TQVALLAVAKGPDRDAGRETLFMPDKEPIKLEPRDPVLYFIQRLRDEAHRFVIGSHRK
                     LRKKDIRE"
BASE COUNT          817 a         1383 c         1353 g          763 t
ORIGIN      
        1 gcgctgctgc ggccgcagga ccgcctcacc gcctgcgaag tcgaacccaa agcgcgcaag
       61 cgcctgatcg aggcgctgcg gcgcgacacc caggcgcggg tggtcgacct cgacggctgg
      121 gtggcgctgc cggccttcgt gccgccgaac gagcggcgcg gcctggtgct gatcgatccg
      181 ccttacgaac aaaacgacga gttcgagcgg atcgccgcgg gctttacgga ggccttcgcg
      241 aaatggccga ccggcagcta tttgctgtgg tatccggtga aaagccggcg cgccaccgac
      301 accctggcgc ggcatgtcgc cgaggtggcc ggcgccggcg cgccgccggg aaaatgcctg
      361 cgtctggaat tcagcgtcgc gccgcaggcc gccggcctga actcggcggg tcttttgatc
      421 gtcaacccac catggacgct ggccggcgaa ctaaaggcca tcctgcccga gcttgaaaag
      481 ccgctcggcc agggcggcgc cggccggttc aggctcgaga cgcccaagcc ttagctgacc
      541 aagccttagc tgatcaagcc ttagctgacc aagccatagt tgaccaagcc ttggctggcc
      601 cggagcccca cccgcggggc cggtggacgc cgcgaaaagt gcgtaggcaa tttgccggga
      661 accgtattat gctccaatcc attaggactg gctttacgtt ccgcttccgc gaatggtagc
      721 ggcggagtga caaggcctca catattttaa aggcggatcg gcgaggtcgc ttgaatccgc
      781 ccggatggcc aagctttccg acagcgaatg tccggtcggg ccggtacgcg cgcgcgtagc
      841 ggcggagcac tacgggatag gagttttccc gatggccatg actggtacgg tcaagttctt
      901 caacggagaa cgtggctatg gcttcatcaa acccgatgac ggcggccgcg atgtgttcgt
      961 tcatatcacc gcggttgagc gggcggggtt gaaggatttg atcgaaggac agcgcatcac
     1021 attcgaggtc gaaccggaca agaagggcaa aggccccaag gcggtcaatc tggtgatttc
     1081 agcttagagc gcgtgtcgcg ctaggtgtgg aatcccgcat tcttgcggcg acaaaggcgt
     1141 agcgctgctc cgatgcctgc gtcgttgccg ggcttgaccc ggcaatccat ccgctttcga
     1201 aaatgcttgt tctgaagatg gatggatacg cggggtcgtc tagaccatga tccgattaga
     1261 ttgaatcgga tcatgatctc atctctttgt ttgagcatga tcttttcgga aaaccggttt
     1321 ccacttttcc ggatcatgct ctagcgcgaa aacgcgcttc gcgcttttgc ccgcgcatga
     1381 cgcctcgttg cagagcctcc aaaaaaatcc cggccgcgcg gcggccggga ggttgtggcg
     1441 gatattttct tgttgcagtt attggaagtt agaagtgata gttgatgccg gcgcgcagcg
     1501 ttccgaaccg gtagccgttc ggtacgccgg tgatggtgaa gttgctgttc gacagatcga
     1561 cgtagagata ttcgaccttc gcgctccagc cgccccggga gaagccggtc tggccgaatc
     1621 cgaattcggc gccgacgcct gcggtccagc ccgcgttggc atgggtttcc gacaggccga
     1681 atgtctcgcc gcgcaattcg ccgaacgcca ggccggcggt gccatagacc aaaatgttgc
     1741 tgaaggcata gccggcccgg ccgcgcacgg tgctgaacca cggattcgag aacttccacg
     1801 gcgcaaatgt ctcgtcagcg ccggtaacct ggaggtcgcc ttcgacgccg aacacccatg
     1861 agggactcgg ttgccagttg tagccggcct ggaccccgcc cacgaaaccc gacggcttgg
     1921 tccgattgtt atcgacggag ccccaggcat agccgagatt gccgccgaga taggggccgg
     1981 cccagctata gcaatcgaac ggctgaacga cggggggggg accagcatag gggggagggc
     2041 gcgatccgta ataaggaatg tcggccgcct gcgccgagac tgcgaagccc gcgcccgtca
     2101 atgcgatcgc ggccaaaacc aacctgctca tgctactctc ccacgcaact gccgcgaccg
     2161 cccggccggc ttgcgcgcgg atgatttgcg caaggcgaac ctgcatggtt acggaactcc
     2221 actgttttct gataaaggtt tatcgagagt cttaagttaa agggttacta agacccgccg
     2281 ccggccactt ggcattgtta acaatgtgtt accggcgagc gctcgcgctc agcttcacct
     2341 cggcccggca ggagcgggcg gccgattccg gaccgggctt gatgctggcg atgcccggtt
     2401 tcagcgctta gtttaggccc atgaccagcg actcccccga caccccaagg gcgccccgcc
     2461 cgccgatttc gcagcccggc tcccgcccgg atttgccgcc ggacccctcc tccggggata
     2521 tcgaccccgc catggccaac gcctgggagg atgacgaggt gctgctgccg gatgttcccg
     2581 aggaggccaa cgaggcggtg ccggaaggtc cgctggcggt cggccacgcc gcgatcgaac
     2641 acgcggtgcg ccatgccccg acctcgcccg gcgtctatcg catgctcaat gccgccagcg
     2701 acgtgctcta tgtcggcaag gcgaaaaacg tccgcaagcg gctcgcgtcc tacgctcgcg
     2761 tcgcggcgcc tcaaccggcg cgcattctgc gcatgatcgc agccaccgtg acggtcgaga
     2821 tcatctccac ctcaaccgaa accgaggcgc tgctgctcga ggccaatctg atcaagcagt
     2881 tgcggccgcg cttcaacgtg cagctgcgcg acgacaagtc gtttccctat atcctgatca
     2941 ccggcgatca ctgggcgccg cagatcctca aacatcgcgg cgcgcaatcg cggcccggac
     3001 aatatttcgg accgttcgcc tcggcgggcg cggtcaatcg caccatcacc gcgctgcagc
     3061 gcgcctttct ggtgcgctcc tgcaccgatg gtttttttga aagccgcacg cggccgtgcc
     3121 tgctctatca aatccggcgc tgttcggggc cctgcaccgg cgagatcgat tttccaggct
     3181 ataccgaact ggtgcgcgag gccaaggatt tcctctccgg ccgcagccgc ctggtaaagc
     3241 aggaactggc cggcgaaatg gaaaaggcct cggccgagct cgcgttcgag accgcggcac
     3301 tttaccgcga ccgcctcgcg gcgctgtcgg cgatccagtc gcagcagggc atcaatccgc
     3361 gcaccgtgga ggaagccgac gtgttcgcca tccaccagga gggcggatat tcctgcgtcg
     3421 aggtgttctt ctttcgcacc ggacagaact ggggcaaccg cgcctatttt ccccgcgcgg
     3481 agaaatcatt caccccggaa gaggtgctgg cctcgttcct cgcgcaattc tacgacgaca
     3541 agccgccgcc gaaattgatc ctgctgtcgc acccaatcga ggagtgcgaa ttgctcgccg
     3601 atgcgctctc catcaaggcc ggcttcaagg tggaagtctc cacgccccgg cgcggcgaaa
     3661 agaaggaact gatcgcgcac gcgctgacca atgcgcgcga agcgctcggc cgcagactgg
     3721 ccgataccgc aacccagggc cggctgctgg ccgggctggt cacgacgctc gggctgccgc
     3781 atccgccgaa gcggatcgag gtctacgata acagccacat ccagggcacc aacgcggtgg
     3841 gcgccatgat cgtggcgggg ccggatggct tcatcaagaa ccagtaccgc aagttcaaca
     3901 tcaagtccga aggcctgacg ccgggcgacg attacgcgat gatgcgcgag gtgctgcagc
     3961 ggcgcttcaa gcgactcttg acgccgcctg tggaaggcga tgccgccaag cccaaggcgg
     4021 acgatgattc gtttccgcaa tggcccgacc tcgtcatcat cgacggcggc cgcggccagc
     4081 tcaatgccgt cagggagatc tttgacagcc tcggcctgac ccaggtcgcg ctgctggccg
     4141 tggcaaaagg accggaccgc gacgccggcc gcgaaaccct gttcatgcct gacaaggagc
     4201 ccataaaact cgagccgcgc gatccggtgc tgtatttcat tcagcggctg cgcgacgagg
     4261 cgcatcgctt cgtgatcggc tcgcaccgca agctgcgcaa aaaggacatc cgcgaa
//
LOCUS       DASWGM010000880         4101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_943466,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000880 DASWGM010000000
VERSION     DASWGM010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943466"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VGA15_23925"
     CDS             complement(<1..249)
                     /locus_tag="VGA15_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9590775.1"
                     /translation="MQLQFVGCGDALGSGGRLNTCFHVTGERVNFLIDCGASSLPALK
                     RLTIARDAIDLILITHFHGDHFGGLPFLLLDAQFTRRTR"
     gene            complement(267..1541)
                     /locus_tag="VGA15_23930"
     CDS             complement(267..1541)
                     /locus_tag="VGA15_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX9590776.1"
                     /translation="MIANLTTPLIGIVSTIAIGRLGDATLLGGVAIASVIFDCLFWLF
                     GFPRMSTLAFTAQSLGAGETEELRPILVRGFVVAALIGAGLVILQIPLAAILLRVMGG
                     SEGVTRAAKIYFTIRIWSAPLALANYVVLGWLVGQARARLALGVQIAINVINVAATAL
                     LVLVFETGIAGAAMAAVAAEAAGLVLGVLIAGRLSRGQPAVPRAALLDRQKLIRMLAV
                     NRDIMIRTASLIAVFLFFTSQGARSGDVTLAANAVLNNFLLISSFFLDGLANAAEQLC
                     GRAYGARSKNEFSGAVRLVVLWGFGFALAVTATFALFGPSLIDIMTASEDVRRGARDY
                     LWFVVLSPMLAVFAFAFDGVYIGATWARDMRNLMMLSLLLFLGAWLALRSFGNAGLWG
                     ALMVHYAARGGLQALRYPALLRASFPRKPCSG"
     gene            complement(1620..1922)
                     /locus_tag="VGA15_23935"
     CDS             complement(1620..1922)
                     /locus_tag="VGA15_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HEX9590777.1"
                     /translation="MPNDTRELPAANDGLYRFLGGSPLTVAFRLILLSILVGVVLAAI
                     GFDPWNIVNSIRLLFQRIWDLGFDAINGLWRYFLLGAVIVIPVWLLSRMFGAPRGR"
     gene            complement(2024..3880)
                     /locus_tag="VGA15_23940"
     CDS             complement(2024..3880)
                     /locus_tag="VGA15_23940"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX9590778.1"
                     /translation="MNRFAELLDRLAYEPGRNNKLRLITSYFREVEDPDRGYALAALT
                     GALSFKHAKPGLIRDLIADRTDPVLFALSYDYVGDLSETVALMWPKRLDHRESSLLGY
                     PSPQPSPTRGEGAAQNPGRSSRKKSEAVATGLSVPSPLEGQGGGYREQDAHDDHPPHD
                     RHNNPPPPTLTEVVTTLRTLGKTELPKQLARWLDELDETGRWALLKLVTGAMRIGISA
                     RLAKTAAAALGDKDPHDVELMWPGLVPPYLELFAWLEGRAEKPVNLDPTPFRPVMLAH
                     AIENTDFATLKAADFVAEWKWDGIRVQAVSGRDARGHMVARLYSRSGEDITKSFPDLL
                     PSLHLPGVIDGELLVLRDGRVQTFNVLQQRLNRKIVSPQLMKEYPIHLRAYDLLGDDS
                     NDLRELPFVDRRARLETFVKRLDDPRIDLSPTIAFDSWDTLAAARKNPAGAGAGEDSE
                     AVEGVMLKHRDAAYLPGRPKGQWWKWKRDPHIIDAVLMYAQRGHGKRSSYYSDYTFGV
                     WTSGEDGEQLVPVGKAYFGFTDEELLQIDRFVRRNTVEKFGPVRHVVHEPDQGLVLEV
                     AFEGLARSPRHKSGVAMRFPRINRLRWDKPPREADRLETLERMLKADAILQA"
     gene            complement(3877..>4101)
                     /locus_tag="VGA15_23945"
     CDS             complement(3877..>4101)
                     /locus_tag="VGA15_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HEX9590779.1"
                     /translation="VELPLVISDHADWNGLTATIDATGAGEIWVTHGQEDALVHWCTA
                     KGLAARPLDLVGYGDDDESDAPAVGEDAEA"
BASE COUNT          811 a         1293 c         1322 g          675 t
ORIGIN      
        1 acgcgtccga cgggtgaatt gcgcatcgag cagcagaaac ggcaggcccc caaagtgatc
       61 gccgtggaaa tgcgtgatca aaatgaggtc gatagcatcg cgcgcgatgg tgagccgctt
      121 cagcgccggc agcgaggatg cgccgcaatc gatcaggaaa ttgacgcgct cgccggtgac
      181 atggaagcag gtgttgagcc ggccaccgga gccgagcgca tcgccgcagc cgacaaattg
      241 caattgcatc ggacgatcct ccagattcag ccggagcacg gcttgcgcgg gaatgaggcg
      301 cgcagcagcg ccggataacg caacgcctgc aagccgccgc gcgcggcata atgcaccatc
      361 agcgcgcccc atagtcccgc attgccgaac gagcgcagcg ccagccaggc gccgagaaac
      421 agcaacagcg acagcatcat caggttgcgc atgtcgcgcg cccaggtcgc gccgatatag
      481 acaccgtcga aggcgaaggc gaacacggcg agcatcggag acagcaccac aaaccataga
      541 tagtcgcgcg cgccgcggcg gacgtcctcg cttgcggtca tgatgtcgat cagcgaaggg
      601 ccgaacagcg cgaaagtcgc ggtcaccgca agggcgaagc cgaaccccca gagcacgacg
      661 agcctcacgg cgccggagaa ctcgtttttt gagcgcgcgc cataggcgcg gccgcataat
      721 tgttcggcgg cattggcaag gccgtcgagg aagaaggaac tgatcaaaag gaagttgttc
      781 aaaaccgcgt tggcggcaag ggtcacgtcg cccgagcgcg cgccttgcga ggtgaagaac
      841 aggaacaccg cgatcaggga cgcggtgcgg atcatgatat cgcggttgac cgccagcatg
      901 cggatcaact tttgccggtc gagcagcgcc gcgcgcggaa cggcgggttg tccacgcgaa
      961 aggcgtcccg cgatcaggac gccaagcacc agccccgccg cttccgcggc gacggcggcc
     1021 atggcggcac cggcgatgcc ggtctcgaac accagcacca gcagggcggt cgccgccacg
     1081 ttgatgacgt tgatcgcgat ctgcacgccg agcgcaagtc tcgcgcgagc ctgtccgacc
     1141 agccagccga gcacgacgta attggccaat gccagcggcg ccgaccagat ccggatggtg
     1201 aagtagattt tcgcggcgcg ggtgacgcct tcgctaccgc ccatcactct gagcaggatg
     1261 gcggcgagcg ggatttgcag gatgaccagc cctgcgccga tcagtgccgc cacgacaaat
     1321 ccgcgcacca ggatcgggcg caattcctcg gtctcgccgg cgccgagcga ctgagctgtg
     1381 aaggcaagcg tgctcatgcg cgggaacccg aacagccaga acaggcagtc gaagataacg
     1441 gacgcgatgg cgacgccgcc gagcagcgtc gcgtcgccca gccgcccgat cgcaatcgtc
     1501 gagacaatgc cgatcagcgg cgtggtcaga tttgcaatca tcgccggtcc ggcgatggca
     1561 aagacctgcg cggtcgtaac cttggatacg gaagtcaacg aaactggcct gcgtgcgatc
     1621 tatcgcccgc gcggggcgcc gaacatccgc gacaacagcc agaccgggat cacgatcacg
     1681 gcgccgagca ggaagtaacg ccacaacccg ttgatggcgt caaagccgag atcccagatt
     1741 cgctgaaata acagccggat gctgttgacg atattccagg gatcgaagcc gatggcggcg
     1801 agcaccacgc cgaccagaat cgacagcagg atcagccgaa acgccaccgt cagtggtgaa
     1861 cctccgagaa agcggtagag gccgtcattg gccgccggca attccctggt gtcgttaggc
     1921 attgatgatc tcccgcaccc attcgcatcc gcactatagc ctgatgtaga ggagatgggg
     1981 aacccgcttg ttggcgtcaa tggccgccgg ccggcggaaa acgctatgcc tgcaggatcg
     2041 cgtccgcctt cagcatccgc tccagcgttt ccagccggtc ggcctcgcgc ggcggcttgt
     2101 cccagcgcag ccggttgatg cgcggaaacc gcatcgccac gccggattta tgccgcggcg
     2161 agcgcgccag gccctcgaag gccacttcca gcaccagccc ctgatccggc tcatgcacca
     2221 cgtgacggac ggggccgaat ttttcgactg tgttgcggcg cacgaagcga tcgatctgca
     2281 acagttcttc gtcagtgaag ccgaaatagg ccttgccgac cggcaccaac tgctcgccat
     2341 cctcgcccga tgtccagacc ccgaacgtat agtccgaata atacgacgag cgcttgccgt
     2401 ggccgcgctg cgcatacatc aacacggcat cgatgatgtg cggatcgcgc ttccatttcc
     2461 accattggcc ttttggacgg cccggcaaat aggcggcgtc gcggtgcttc agcatcacgc
     2521 cttcgaccgc ttcggagtct tcgcccgcgc cggcgccggc gggatttttt cgcgccgcgg
     2581 ccagcgtgtc ccaactgtcg aaggcgattg tcggcgacag gtcgatgcgc gggtcgtcca
     2641 gtcgtttgac gaaggtctcc agcctcgcgc gccggtcgac aaatggcaac tcgcgcagat
     2701 cattgctatc atcgccaaga agatcatagg cgcgcaggtg gatcgggtat tccttcatta
     2761 gttgtggcga gacgattttg cggttgagac gttgctgcag cacgttaaaa gtttgcaccc
     2821 ggccgtcacg cagcaccaat agctcgccgt cgatgacgcc gggcaaatgc aaggacggca
     2881 gcaggtcggg aaaacttttg gtgatgtcct cgccgctgcg cgaataaagc cgcgccacca
     2941 tgtggccgcg cgcgtcgcgg ccgctgaccg cctgcacccg aatgccgtcc catttccatt
     3001 cggcgacgaa atcggcggcc ttgagggtcg caaaatccgt gttctcgatc gcatgcgcca
     3061 gcatcaccgg gcggaacggc gtggggtcga gattgacagg tttttcggcg cggccttcca
     3121 gccacgcgaa cagctcgaga tagggcggga ccaggcccgg ccacatcaat tcgacatcgt
     3181 gcgggtcctt gtcgccgagt gctgcggccg ccgtctttgc aagccgcgcc gagatcccga
     3241 tccgcatcgc gccggtgacg agtttcagca gtgcccagcg gccggtctcg tcgagttcat
     3301 cgagccagcg cgcgagctgt tttggcagct cggtcttgcc gagggtgcgg agtgtggtga
     3361 cgacttcggt gagggtgggt gggggagggt tgttgtggcg gtcgtgaggt gggtggtcat
     3421 catgagcatc ttgctcgcgg taccctcctc cctgaccctc aaggggggag ggaacagaaa
     3481 gacctgttgc cacggcttca gactttttgc gagacgaccg cccaggattc tgcgcggctc
     3541 cctcccccct tgtgggggag ggttggggag aggggtaacc cagcaacgag gattcgcgat
     3601 ggtccaacct cttcggccac atcagcgcga ccgtctccga caggtcgccg acgtaatcat
     3661 acgataaggc gaacagcacc ggatcggtac gatcagcgat caggtcgcgg atcagcccgg
     3721 gttttgcgtg cttaaacgac agcgcgccgg tgagggctgc gagcgcgtag ccgcgatcgg
     3781 ggtcttcgac ctcgcgaaaa tagctcgtga tcagccgcag cttgttgttg cggccgggct
     3841 cataggcgag gcgatcgagc aattcggcaa agcggttcat gcctcggcgt cctcgcctac
     3901 cgccggcgca tcgctttcgt cgtcgtcgcc gtagccgacc agatcgagcg gccgcgcggc
     3961 caggcccttg gccgtgcacc agtgtaccag cgcgtcttcc tggccgtggg tgacccagat
     4021 ttcaccggca ccggtggcat caatggtcgc ggtcagtccg ttccagtcgg cgtggtccga
     4081 aatcaccagc ggcagctcga c
//
LOCUS       DASWGM010000881         5408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2717329,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000881 DASWGM010000000
VERSION     DASWGM010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2717329"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="VGA15_23950"
     CDS             complement(<1..1679)
                     /locus_tag="VGA15_23950"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX9590780.1"
                     /translation="MTTATALKPSVPKQPPSPNTTGRVIAAAKAAYQYPLIIKHLWHA
                     PLLQARDQEIVYRDLRRFTYRELRERVGRLASALAGVGVRPGDTVGVLDWDSNRFLEA
                     FFAVPMMGAVLQTVNVRLSPEQIAYTINHAGSSVLLVNDEFVPLLQDLMPQLPKVTRL
                     VAMSDRSAPRTGGLSLEGEYEALLAASSPDYRFPDFDENTQATTFYTTGTTGLPKGVY
                     YSHRQLVLHCLSELAFLGMAGKHGRFSRDDVYMPMTPMFHVHAWGFPWAATLAGVKQV
                     YPGRYDPALLVRLIATEGVTFTHGVPTLLQMLLDAAAAAKAHLGGLKMVIGGSELHKA
                     LAKQALAAGIDVYASYGMSETGPIVAVAQVMTKDLGGDPEREVDLRVRAGITAPLVDA
                     RIVGQDMKELPHDGETAGELVLRSPWLTQGYVDNPEASEALWAGGYLHTGDIAVVTPD
                     GSIRITDRMKDVIKSGGEWVSSLQIEELIGQYPGVKEAAVIGLPDEKWGERPFALVVK
                     DSDHASRVLEADIKAHLQAFASRGVISKYGIPEKIQFVDGLAKTSVGKIDK"
     gene            complement(1700..3418)
                     /locus_tag="VGA15_23955"
     CDS             complement(1700..3418)
                     /locus_tag="VGA15_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX9590781.1"
                     /translation="MTRRVADLLVETLQASGVKTCYGIVGDTMNRIAHAIDRSQIDWV
                     HVRHEEAGAFAAAAEASLTGRLTACAGTCGPGSLHFINGLYEANRNRAPVVLIATQIV
                     RQDLGFESIQEIDFDDVFKGCSVYCEMILTPEQARRKTVAACQAALTKRGVAVLVVPA
                     DVANAASHDEVPYAVHARRPLIRPSDADLDEIAAILNKSDGITIYAGAGCAGAHDEVV
                     ATAARLMAPVAHTSRGKDFLEYDNPYNVGMTGIIGGRAGYHAVLDCNVLLLLGADFAW
                     SQFYPNRATIIQIDIDPTHIGRRHPVAIGAVGNIKTTLQALLPRLDEHEDDGFLTTHV
                     KRFQKDVAAAKAETVSGPDAVISGTYLTKVINKYAAEDALFAADDGTATVWMLRHIDT
                     GGKRRTFASLLHGTMAGGMASALGLQKCQPGRQVICLAGDGGFAMLLGDLLTTVQENL
                     PIKIVVYDNGKLGFVEIEQKAAGLVPVYTDLKNPDFGEVAKAMGLWGDSISKAGDLEE
                     AVQTWLAQPGPAVLHVKVNPMQLVMPPSPLVAPEAVVGMAVYSARAMLHGKGHDVWEM
                     MVENIP"
     gene            3794..>5408
                     /locus_tag="VGA15_23960"
     CDS             3794..>5408
                     /locus_tag="VGA15_23960"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9590782.1"
                     /translation="MTLARKWANSRTRGRKLHLTGAKARVGRATKTRADLEQQLKACR
                     REIAHARERLAEATMQQTATSEMLRLISNSPIQSVLDAVAEHAARLCDASNARIWRLE
                     DNLLRLVASYGESSATMDGREGLPVDRDTVTGRAAHDRRTIHVHDIAAEDSEYPVGSR
                     IVKGQGWRTTLATPLLREGTPMGTILVRRMEVRPFSDKQIALLETFAAQAAIAIENVR
                     LFEAEKQRTLALAHANRDLAEREAKIRRLVDSNIIGIFIWDFDGRILEANDAFLRMVN
                     YDREDLVSGRMQWTELTAPDWRDRNNARIEQQKSSGHFPPFEKEYTRKDGTRVPVLMG
                     GATFEEGGNQGMAYALDLTERKRAEEALRESEAKLRDYAETASDWFWEIGPDYKFTLL
                     TENAFGSDPAERIGTACWAHALDLETEPEKWRLFRETLDAREPFRDFVYCSVGGNGLP
                     MYVKASGKPVFDANGEFRGYRGTGSDVTERQRAQEERERLRQLEADLAHMNRLGMMGE
                     LAASLAHEITQPIASARNNARAALNFLDQR"
BASE COUNT         1015 a         1697 c         1684 g         1012 t
ORIGIN      
        1 ctcttgtcga tcttgccgac gctcgtcttt gccagaccgt cgacgaactg gatcttctcg
       61 ggaatgccgt atttcgagat gacacctcgg gacgcgaacg cctgcaagtg cgccttgata
      121 tcggcttcga gcacgcgact ggcgtgatcg ctgtccttga cgacgagcgc gaaaggccgt
      181 tccccccact tttcatccgg gagcccgatc acggcggctt ccttcacgcc gggatactgc
      241 ccgatcagct cctcgatctg cagcgatgat acccattctc cgccgctctt gatgacatcc
      301 ttcattcggt cggtaatccg aatggagccg tccggcgtga cgacggcgat atcgcccgtg
      361 tgcaggtacc cgccggccca gagcgcttcg gacgcttcgg gattgtcgac gtaaccttgc
      421 gtgagccatg gcgaccgcag caccagctcg cccgcggtct cgccatcatg cggcagttcc
      481 ttcatgtcct gcccgacaat acgtgcgtcg accagcggcg ccgtgatccc ggcacgaacg
      541 cggagatcca cttcccgctc cggatcgccg ccgaggtcct ttgtcatgac ctgcgcgacc
      601 gcgacgatcg gcccggtctc ggacattccg tagcttgcgt agacgtcgat tccggctgcc
      661 agggcctgct tcgccaacgc cttgtgcagc tccgatccgc cgatgaccat cttcaatcca
      721 ccaagatgag ctttggcggc ggcagcggcg tcgagcagca tctgcaggag agtaggtacg
      781 ccatgcgtga aggtgacgcc ctcggtcgcg atcagcctga cgagcaatgc cgggtcgtag
      841 cggccggggt agacttgctt cacgccggca agcgtcgccg cccacggaaa tccccaggcg
      901 tgcacgtgaa acatcggcgt catcggcata tagacgtcgt cgcgggagaa ccgcccgtgc
      961 ttgccggcca tgccgaggaa ggccagctcg gacaggcagt ggagaaccag ctgccgatga
     1021 ctgtaataga cccccttggg caggcccgtt gtgccggtcg tgtagaaggt ggttgcctgg
     1081 gtattctcat cgaagtccgg aaagcggtag tccggcgatg aagcagccag gagcgcctcg
     1141 tactcccctt ccaacgacag gcctccggtc cgcggcgcag agcgatccga catcgcgaca
     1201 agtcgcgtca ctttggggag ttgcggcatg agatcttgca gcagcgggac gaactcgtcg
     1261 ttgaccagga gcaccgatga gccggcgtga ttgatcgtgt aggcaatttg ctccggcgac
     1321 aggcggacat tgacggtctg cagcaccgct cccatcatcg gcaccgcaaa gaacgcctcg
     1381 agaaatctgt tactgtccca gtccagcacc ccgaccgtat cgccgggccg gacgcccact
     1441 cctgcaagcg cggaggccag gcggccgacc cgctccctga gctcgcggta agtgaagcgt
     1501 ctcaagtctc gatagacgat ctcctgatcg cgagcctgga gcagcggagc atgccacaaa
     1561 tgtttgatga tgagcggata ctgataggcg gcctttgcgg ccgcgataac cctgcccgtc
     1621 gtgttcggcg acggaggctg cttcggtacc gaaggtttga gtgccgtggc ggtggtcatt
     1681 tggcggtctc ctgatgagat caggggatgt tctccaccat catttcccag acgtcgtggc
     1741 ctttgccgtg cagcattgcc ctggcgctgt agaccgccat cccgacgacc gcctccggtg
     1801 caacgagggg cgagggcggc atcaccagct gcattgggtt caccttcaca tgcagcaccg
     1861 ctgggccggg ctgcgcgagc caagtctgga cggcctcctc gagatcgcct gccttcgaga
     1921 tgctgtcccc ccaaaggccc atcgccttcg cgacttcgcc gaagtcgggg ttcttcaagt
     1981 ccgtatacac cggcaccaag ccggctgcct tctgctcgat ctcgacaaat ccgagcttgc
     2041 cgttgtcgta gacgacgatc ttgatcggca agttttcctg caccgtcgtc aggagatcgc
     2101 cgagcagcat ggcgaacccg ccgtcgcccg ccagacagat cacttgtcgg cccggctgac
     2161 atttttgcag tccaagcgcc gacgccatcc cgcccgccat ggtgccgtgg agcaagctgg
     2221 caaaggttcg ccgtttgccg ccggtgtcga tatggcgaag catccagact gtcgctgtcc
     2281 cgtcatccgc cgcgaacaag gcatcctcgg ccgcgtactt gttgataacc ttcgtcaggt
     2341 aggtgcccga aatgacggcg tccggacccg acacggtttc agccttcgcc gccgccacgt
     2401 ccttctggaa acgcttcaca tgggtcgtca ggaatccgtc atcctcgtgc tcatcgagac
     2461 gaggtagcag agcctgcaag gtcgtcttga tattgccaac cgccccgatt gcgacgggat
     2521 gacgtcggcc tatatgggtg ggatcgatgt cgatctggat gattgtcgcc ctatttggat
     2581 agaattggct ccaggcgaaa tctgccccga gtaggagcag cacgttacag tcgaggacgg
     2641 catgatagcc ggcgcgtccg ccgatgatgc ctgtcatgcc gacgttgtaa ggattgtcgt
     2701 actcgaggaa gtccttgccg cgagacgtgt gggctactgg cgccatcaac cgcgccgccg
     2761 tggcgacgac ctcgtcatgc gcacccgcgc agccggcgcc cgcataaatc gtgattccgt
     2821 cgctcttgtt gagaatggcc gcgatctcat cgaggtccgc gtcactcggg cgaatgagcg
     2881 gacgccgcgc atgaacagca tacggcactt cgtcgtgcga ggcggcattg gcgacgtccg
     2941 ccggcacgac gagcacggcg acaccgcgtt tcgtcagggc tgcctggcag gctgcgactg
     3001 tcttgcggcg ggcctgctcg ggtgtcagga tcatctcgca atagacgctg cagcccttga
     3061 acacgtcatc aaaatcgatc tcttggatgg actcgaatcc caggtcctgc cgcacgatct
     3121 gggttgcgat caggacgacc ggtgcgcggt tgcggttggc ttcatagagg ccgttgatga
     3181 aatggagact gccggggccg caggttcccg cgcaggccgt gagacgccca gtgagcgacg
     3241 cctcggcggc cgccgcgaaa gcgccggcct cttcatgccg cacatggacc caatcgatct
     3301 ggctgcggtc gatggcgtgg gcgatccggt tcatcgtgtc gccaacgatg ccgtagcacg
     3361 tcttcacgcc ggatgcttgc agcgtttcga ccagtaaatc ggccactcgc ctggtcatga
     3421 cggcgcctcg gcggatgctg cgccagttgc gcggggtgtt cgtggagtgg cggttgccat
     3481 ttgagtctcc attctctcga cttgcccgcg ctcgcctcta gctaaccccg cgcgcttcct
     3541 ggcgcgagtg aagttcggca aatcgctcca aattcctgct aacgagactg cggatcgtca
     3601 gatccacaca gcgcgcactg gccggcatga gcatgctcgc gtgaaccgca ctcgttccac
     3661 gcttcttgtg tccgatgagc ttctcgaccg agatcaatat gctgacgcgc gctctgggcc
     3721 gtgtgtgcta ttctccccgc agccccccgt ccccggatgt aacgaccagc attggggccg
     3781 tctggcgatg gagatgacct tggctcgcaa atgggcgaat agccgaacgc gaggtcgcaa
     3841 gcttcatttg accggcgcga aggcgcgcgt tggtcgagct accaagaccc gcgccgatct
     3901 cgaacagcag ctcaaagcat gcagacggga gatcgcccac gcgcgtgagc gccttgccga
     3961 ggccacgatg caacaaactg ccacctccga gatgctgcgc ctcatctcga actcgccgat
     4021 tcagtcggtg ctggacgcgg tggccgaaca tgccgcacgc ctgtgcgacg cgagcaatgc
     4081 gaggatctgg cgcctggagg acaaccttct tcggctggtg gcttcatacg gcgaaagttc
     4141 ggcgactatg gatggccgcg agggccttcc cgtagaccga gacacggtga ctggacgagc
     4201 cgcacacgat cgtcggacga ttcatgtcca tgatatcgcc gcggaggaca gcgagtaccc
     4261 ggtgggtagc aggatcgtga aaggccaggg ttggcgcacc acgcttgcga cgccgttgtt
     4321 gcgcgagggt acccccatgg gaacaattct agtacggcga atggaggtcc gaccgttctc
     4381 cgacaaacag atcgcgctgc tcgagacctt cgccgcccag gcggcgatcg cgatcgaaaa
     4441 tgtgcgcctg ttcgaagccg agaagcaacg cacgctggcg cttgcgcacg ccaaccgcga
     4501 tctcgcagaa cgcgaggcga aaattcggcg cctagtcgac tcgaacatca tcggaatttt
     4561 catctgggat ttcgacggtc gcattctcga ggccaacgac gcgtttcttc gcatggtgaa
     4621 ctacgaccgc gaagacctgg tctcgggccg catgcagtgg acagagctga cggcgcccga
     4681 ctggcgcgac aggaataacg caaggatcga acagcaaaaa agcagcggcc actttccacc
     4741 gttcgagaag gaatacaccc ggaaggatgg aactcgggta ccagtgctga tgggtggggc
     4801 aacattcgaa gagggcggca accagggtat ggcctatgcg ctcgatttga ccgagcgcaa
     4861 gcgtgccgag gaagcattgc gggagagcga ggcgaagctc cgcgattacg ccgaaactgc
     4921 ctccgattgg ttctgggaaa tcggtccgga ctacaaattc acactgttga ccgagaatgc
     4981 gtttggttcg gacccggcgg agcgaatcgg cacagcgtgc tgggctcacg ctctcgacct
     5041 tgaaacggaa ccggagaagt ggcggctttt tcgggaaact ctcgatgcac gtgagccgtt
     5101 ccgcgacttc gtctattgct ccgtgggcgg caacggcctg ccaatgtacg tgaaggccag
     5161 cggcaagccg gtcttcgacg ccaacggaga attccgtggc tatcgcggca ccggcagcga
     5221 tgtgacggag cgccagcggg cgcaggagga gcgcgagagg ctgcgccagc tggaggcgga
     5281 cctcgcgcac atgaaccgcc tgggcatgat gggcgaactg gccgcctcgc tggctcacga
     5341 gatcacgcaa ccgatcgcca gcgcgcgcaa caacgcccgt gcggcgctga attttctgga
     5401 ccaacggc
//
LOCUS       DASWGM010000882         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1198519,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000882 DASWGM010000000
VERSION     DASWGM010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198519"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VGA15_23965"
     CDS             <1..879
                     /locus_tag="VGA15_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9590783.1"
                     /translation="LGPSVDEFEIGERVSVEIHAGCGQCKRCRQGMYTSCLNYGRAEK
                     GHRANGFTTDGGFAEYAINHINTLARVPDTMSDAEATLVVTAGTSMYGLTELGGLVAG
                     ESVVVIGPGPIGLLAVAVAKALGASPVILTGTRNKRLAIGKELGADRVINIAEENAVE
                     VVKQLTGGVGADYVVECAGTEATVNQAIHMTNRGGKICLAAFPHDPVTTDLAHLVKNN
                     IYIYGIRGEGRSATRRAMALMAEKRFDATKIHTHTFALDDLPTALRYARDRVEDAIKV
                     VVTSRQASAPTNAAAK"
     gene            complement(898..>1644)
                     /locus_tag="VGA15_23970"
     CDS             complement(898..>1644)
                     /locus_tag="VGA15_23970"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9590784.1"
                     /translation="LAGFASISEKMTPAELVAFMNEYLSAMTDIIESRGGYVDKYIGD
                     SIVAVFGAPADDSDHASNAARAALGCRARLDELNRDSAAFKGYDVAHRMGLNSGAALV
                     GNIGSRRRFNYSVMSDAVNVASRLEGANKYYRTTIAASEMTVALTGSTFTWRELDAIR
                     VKGRATPVKIYELLAEAGEETPQQAAAATAYVQGLAYWRNREFDAAAKCFGRVADVDR
                     PSALFLNRANAFASHPPGPDWEPVTTLDAK"
BASE COUNT          352 a          522 c          476 g          294 t
ORIGIN      
        1 ctcggaccca gcgtcgacga attcgaaatc ggcgaacgcg tcagcgtgga aattcacgcg
       61 ggctgcggcc agtgcaagcg atgtcgtcag ggcatgtaca cctcatgcct gaactacggg
      121 agggccgaga agggtcatcg cgccaacggc tttaccaccg atggcggttt cgccgaatac
      181 gcaatcaacc atatcaacac gctggcgcgg gtgcccgaca cgatgagcga cgcggaggcg
      241 acgcttgtcg tgacggccgg cacttccatg tacgggctga ctgaactggg cggcctggtt
      301 gccggggaaa gcgttgtcgt gattggtccg ggacccatcg ggctgctcgc agtggccgtt
      361 gcaaaagcgc ttggcgcaag ccccgtcatt ctaacgggaa cccgcaacaa acgcctcgcg
      421 atcggcaagg agctcggcgc ggatcgcgtc atcaacatcg ccgaggagaa tgccgtcgag
      481 gtcgtaaaac agctgactgg cggggtcggc gcggactatg tggtcgaatg cgccggcact
      541 gaagccactg tcaatcaagc catccacatg accaatcgcg gcggaaaaat ctgcctggcg
      601 gccttcccgc acgatccggt gacgaccgat ctggcccatc tggtcaagaa caatatctac
      661 atctacggca ttcgcggcga aggccgcagc gctacacgcc gtgcgatggc gcttatggca
      721 gaaaagcgct ttgatgcgac caaaatccat acccatacct tcgcactcga cgacctcccg
      781 accgcgctcc gatacgcacg cgatcgagtc gaagacgcca tcaaggtggt tgttaccagc
      841 aggcaagcga gcgcgccgac aaacgcagcg gcaaagtaga ctttcaagcg caatctccta
      901 ctttgcatcc aaagtagtga ccggttccca atccggaccg ggtggatggc ttgcaaatgc
      961 gttcgcccgg ttgagaaaca gcgcggacgg cctgtcgaca tcggcaaccc gcccaaagca
     1021 ttttgccgcg gcatcaaatt cacggtttcg ccaatacgcg agcccctgga cataggcagt
     1081 ggcagccgcc gcctgctgcg gcgtctcctc ccccgcctcc gcaagcagtt cgtagatctt
     1141 taccggggtg gcgcgtccct tgacccggat ggcgtcaagt tcgcgccatg taaatgtcga
     1201 tcccgtgagc gcaaccgtca tttccgaggc ggcgatcgtg gtgcggtaat acttgttcgc
     1261 accctcgagc cgcgacgcga cgttcaccgc gtcgctcatc acggaatagt tgaagcgccg
     1321 ccgcgaaccg atattgccta ccagtgccgc acccgaattg agccccatcc gatgcgccac
     1381 gtcgtaaccc ttgaaggcag cggaatctcg attgagctcg tcgagacgcg cccggcaacc
     1441 gagcgccgca cgcgccgcat tgctcgcatg gtcgctatcg tcggccggcg cgccgaacac
     1501 cgcaacgatc gagtcgccga tatatttgtc gacatagcca cctctacttt cgatgatgtc
     1561 ggtcatggcg gaaagatact cattcatgaa cgccactagt tccgccggcg tcattttctc
     1621 agagattgaa gcaaagcccg ccaa
//
LOCUS       DASWGM010000883         4442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2483929,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000883 DASWGM010000000
VERSION     DASWGM010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483929"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            551..1813
                     /locus_tag="VGA15_23975"
     CDS             551..1813
                     /locus_tag="VGA15_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9590785.1"
                     /translation="MTTLHADFASQDYFRDPGAAIGKLRTLGPVVQIRFPIVGKVWTT
                     TTQALADQVLKDTETFTIRSDDGTVAGLQWWMPGIVRTFANSMLSMDEPDHKRLRDIV
                     DEAFRRRAVLDMEPHIQAIGDELADELFAEGSPADLVERYARKLPLSVICELLGLPLA
                     DRPKFTAWAAGFTRFTGTLGFLSMIPNVFAMKRYIEQHLETVRRQGGEGLIAEIVRVE
                     KSGGQISPNEIVAMVFLLLLAGHETTTHLISGSVHELLKNPDLRDWLEEDWSRVDLAV
                     EEFLRFITPVQFTKPRYVRKDIELGGVRLRKGEKIMVMLAAANMDPQANPHPERLDLQ
                     RRPNRHIAFGTGIHFCLGHQLARIEGRCALKSLLQRWPALTLAVDESEITWRKRPGIK
                     AIDHLPVAAGRERPHQVARSVGGREAAG"
     gene            2154..3878
                     /gene="ilvD"
                     /locus_tag="VGA15_23980"
     CDS             2154..3878
                     /gene="ilvD"
                     /locus_tag="VGA15_23980"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX9590786.1"
                     /translation="MDVKTSIKKRLPSRHVTEGPERAPHRSYLYAMGLTSQQIHQPFV
                     GVASCWNEAAPCNISLMRQAQAVKKGVASAGGTPREFCTITVTDGIAMGHDGMRSSLP
                     SRECIADSVELTVRGHAYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGNF
                     RGQQVTVQDMFEAVGKHSVGEMSDADLDEIERVACPSAGACGAQFTANTMATVSEAIG
                     LALPYSAGAPAPYEIRDAFCMTAGEKVMELITLNIRPRDIVTRRSLENAAAVVAASGG
                     STNAALHLPAIAHECGIKFDLFDVAEIFKKTPYVADLKPGGRYVAKDMFEVGGIPLLM
                     KTLLDNGHLHGDCLTVTGRTIAENLKSVKWNPHQDVVRSADKPITVTGGVVGLKGNLA
                     PEGAIVKVAGMSNLKFTGPARCFDREEDAFESVQKKTYKEGEVIVIRYEGPRGGPGMR
                     EMLSTTAALTGQGMGGKIALITDGRFSGATRGFCIGHVGPEAAVGGPIALLRDGDIIE
                     IDADAGILNVKLTDTELAERKTSWKPRATNHTSGALWKYAQQVGPAVDGAVTHPGGEH
                     EKQCYADI"
     gene            3865..>4442
                     /locus_tag="VGA15_23985"
     CDS             3865..>4442
                     /locus_tag="VGA15_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9590787.1"
                     /translation="MRIFKGTIFALILGVTPVAGPLFAFDGAPATQDPVMPAVSGQPG
                     AVAALKKAVPPSTSDTSLTSLQYAAEEGHPVAQWKLGKMYADGVGVTQDDLRAFEYFS
                     RIANAHAEDSPSAPQATIVANAFVALGRYYLSGIPNSKITSDPERAREMFSYAASYFG
                     NADAQYDLARLYLRGLSSSREDFRYGARWLGLA"
BASE COUNT          861 a         1329 c         1431 g          821 t
ORIGIN      
        1 ggaaggcaag tcggcccaac cgggcgcgga cgagccagat atccgcgaga tgacggtggc
       61 aaaagagccc ggtcaccggg gagagcacga cataagctgt taaaaccatt gcgtgcggga
      121 atgccgggtg attccggtgg acctgtggct tctacccctg tgctttctac attgcacagg
      181 gggctgcggg tgcagcgggc acccggcatt ccccacgccc tcaaaggggg cgagagataa
      241 atgcacgact cgggcgcttc gcgccgcggg gtcgcgaacg cgcgtctgga gttgccgtca
      301 ttacgagcgg cccagtcgcc ggggcgagac gaaatcgaga ggcgccctta tggaaccctt
      361 atgcgctctc gacccgcctg atcttgaatt acgacaacga gcttcgcatc tttcggagga
      421 gcccggcggg gccaaaaact gaactgaacg caagcgacgg cgtttcttgt ccggggggcc
      481 tgagagtggc cgcgccggca cacccgcgca gcaccttcgg gatgcccggc tttcggtaag
      541 ggaatctctc atgacgacgc tccatgcgga cttcgccagt caggactact ttcgcgatcc
      601 cggtgccgcg atcgggaaac ttcgtaccct gggcccggtt gtgcagatcc ggttcccaat
      661 cgtcggcaag gtctggacga cgacgaccca ggcgctcgcc gaccaggtgc tgaaggacac
      721 cgaaaccttc accattcgca gcgacgacgg cacggtcgcc ggattacagt ggtggatgcc
      781 gggcatcgtg cgcacgtttg ccaacagcat gctgtcgatg gacgagccgg atcacaagcg
      841 gttgcgcgac atcgtggacg aggcatttcg ccgccgcgcc gtgctcgaca tggagccgca
      901 tattcaggcc attggtgacg aactcgccga tgaactgttt gcagaaggaa gcccggccga
      961 cctcgtcgag cgctatgcgc gcaagctgcc gctgtcggtg atctgcgagc ttctcggcct
     1021 gccgcttgcc gaccggccaa aattcactgc ctgggccgcc ggcttcaccc gcttcaccgg
     1081 cacgctcgga tttctcagca tgatcccgaa cgtctttgca atgaagcgct atattgaaca
     1141 gcatctcgaa accgttcgcc ggcagggcgg tgaagggttg atcgccgaga tcgtgcgcgt
     1201 ggagaaaagc ggcggacaga taagccctaa cgaaatcgtg gcgatggtgt tcctgttgct
     1261 gcttgccggg catgagacca ccacgcacct gatcagtgga tcagtccatg agcttctgaa
     1321 aaatcctgat ctgcgcgact ggctcgaaga agactggagc cgcgtcgatc tggcggtgga
     1381 ggaatttctg cggttcatca caccggtgca attcaccaaa ccgcgctacg tgcggaaaga
     1441 tatcgaactc ggcggcgtca gactgagaaa gggcgagaag atcatggtca tgctggcagc
     1501 cgccaacatg gaccctcagg ccaatcccca tccggaacgg ctcgatttac agcgaaggcc
     1561 gaaccggcac atcgcattcg gcaccgggat ccatttctgc cttggccatc agctcgcgcg
     1621 tatcgaaggt agatgcgcgc tgaaatctct gttgcagcgt tggcccgcct tgacgctcgc
     1681 cgtcgatgaa tcggaaatta cctggcgcaa acggcccggc atcaaggcga tcgaccatct
     1741 gccggtcgcg gccggccggg aaaggccgca tcaggtcgcg cgaagtgtgg gcggtcgaga
     1801 agcggctgga taggcctggc gggcggcggg tggcaaaagg ccgagattgc gaacgatggg
     1861 cgatgatcca gccgccaccg tcagggtcga tcggcgcgga ctgtttgcaa cctgagcgac
     1921 gaagcaaatt tggccgcaac catggttgct cccgcgccac gattgttgca gacccgcaac
     1981 acttagcgaa catttgggtc aagggcggcc tggctattgc ctgcgccgct ttttagccac
     2041 acccctccgt gacatcatat gtaggaaaaa attgccgctt cgttcgcgaa ggcgatggga
     2101 aatttggggt tccccgggga accgaagtgt gtagctgggg aaacaggtta gcaatggacg
     2161 ttaagaccag catcaagaag cgattgccga gccggcatgt gacggagggc ccggagcggg
     2221 cgccccatcg ctcgtatctc tatgccatgg ggctgaccag ccagcagatc caccagccct
     2281 tcgtcggcgt ggcgtcgtgc tggaacgagg ccgcgccctg caatatctcg ctgatgcgtc
     2341 aggcccaggc ggtcaagaag ggcgttgcct ccgccggcgg caccccgcgc gaattctgca
     2401 ccatcaccgt caccgacggc atcgcgatgg gccatgacgg catgcgctcg tcgctgccgt
     2461 cccgcgaatg catcgccgat tcggtcgaat tgaccgtccg cggccacgcc tatgacgcct
     2521 tggtgggcct cgccggctgc gacaagtcgc tgccgggcat gatgatggcg atggtccgcc
     2581 tcaacgtgcc gtcgatcttc atctatggcg gctcgattct tcccggcaat ttccgcggcc
     2641 agcaggttac ggtgcaggac atgttcgagg cggtcggcaa gcattcggtc ggcgagatgt
     2701 ccgatgccga cctcgacgaa atcgagcggg tggcttgccc gtcagccggc gcgtgcggcg
     2761 cacaattcac cgccaacacc atggcgacgg tgtccgaggc catcgggctt gcgctgccct
     2821 attcggcggg cgcgcccgcg ccttacgaga ttcgcgatgc gttttgcatg accgcgggcg
     2881 agaaagtcat ggagctgatc accctgaaca tccgcccgcg cgacatcgtc acccgcaggt
     2941 cgctggaaaa cgccgcggcg gtggtcgccg catcgggcgg atcgaccaat gctgccctgc
     3001 atctgccggc gatcgcccat gaatgcggaa tcaagttcga cctgttcgat gtcgccgaaa
     3061 tcttcaaaaa gacaccttat gtcgcggatt tgaaaccggg cggtcgctat gtcgccaaag
     3121 acatgttcga ggttggtggc ataccgcttt tgatgaagac gttgctcgat aacggccatt
     3181 tgcacggcga ctgcttaacg gtaactggcc gaacgatcgc cgaaaacctc aagagtgtga
     3241 aatggaatcc gcaccaggat gtggtgcggt ccgccgacaa gccgatcact gtcaccggcg
     3301 gtgtggttgg cttgaagggc aatctcgcgc ccgagggcgc aatcgtgaag gtcgcgggca
     3361 tgtcgaattt gaaatttacc ggtcctgccc gctgcttcga tcgcgaagag gatgccttcg
     3421 agtcggtcca gaagaagacc tacaaggaag gcgaagtcat tgtgattcgc tatgaagggc
     3481 cgcgcggcgg tcccggcatg cgcgaaatgc tctcgaccac ggcggcgctg accgggcagg
     3541 gcatgggcgg caaaatcgcg ctcatcactg acggccggtt ttcgggggct acccgcgggt
     3601 tctgcatcgg ccatgtcggg ccggaagcgg ccgtgggtgg gccgatcgcg cttctgcggg
     3661 acggcgatat catcgagatc gacgcggatg ccggaatact taacgtaaaa ttgaccgata
     3721 cggagctcgc cgaacgtaag accagttgga agcctcgcgc gaccaatcat acgtcaggtg
     3781 cgctgtggaa atatgcccag caggttgggc cggcggtgga tggcgccgta acccatccgg
     3841 gcggggagca tgagaaacag tgctatgcgg atatttaagg gaaccatttt tgcgttgatt
     3901 ttaggggtca ctccggtggc cgggccgttg tttgccttcg acggggcgcc ggctacccag
     3961 gatccggtga tgccggcggt ttcgggtcag cccggggccg tggccgcgtt gaagaaggcg
     4021 gtcccgcctt cgacgtccga cacctcgctg acttcgctgc aatatgccgc ggaggaaggt
     4081 catcccgtcg cgcaatggaa gttggggaag atgtacgccg atggcgttgg cgtcacccag
     4141 gacgatcttc gcgccttcga gtatttcagc cgcatcgcca atgcgcacgc ggaagacagc
     4201 ccgtcggcgc cgcaggcgac aatcgtcgcc aacgcctttg tggcgctggg gcggtactac
     4261 ctcagcggca ttcccaactc gaagatcacc tccgatccgg agcgggccag ggagatgttc
     4321 tcctatgccg catcgtattt cggcaatgcc gacgcgcagt atgatctggc ccggctctat
     4381 ctgcggggcc tcagctcgtc gcgggaggac ttccgctatg gcgcccgctg gctcggcctt
     4441 gc
//
LOCUS       DASWGM010000884         5403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1608686,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000884 DASWGM010000000
VERSION     DASWGM010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608686"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /gene="panC"
                     /locus_tag="VGA15_23990"
     CDS             complement(<1..844)
                     /gene="panC"
                     /locus_tag="VGA15_23990"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX9590788.1"
                     /translation="MSRSPMIVRTVPALRRALDSIRAKKNTTALVPTMGALHDGHVSL
                     VRLAKRRAKRVIVSIFVNPTQFAPTEDFGSYPRTWEADVARLAAENVDLIWNPDVKTM
                     YPDGFATRILTEGPATAGLEDRFRPHFFGGVTTVVGKLFTQCRPDFAIFGEKDFQQLR
                     VVTRMAGDLDLGVRVIGSRTVRERDGLAMSSRNVYLSPEERLIAPTLYRTMKESASSL
                     RAGDNIEAAMAGGAELITNAGFVLDYYEPRHADTLAPIASAKDGPVRILVAARLGKTR
                     LIDNI"
     gene            1126..2049
                     /locus_tag="VGA15_23995"
     CDS             1126..2049
                     /locus_tag="VGA15_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEX9590789.1"
                     /translation="MLMQTSQGQSGSAHVVVLGNEKGGSGKSTTALHVAVALLKAGQR
                     VATIDLDCRQQSFTRYINNRRAWARRTGLDLELPVHCCIKLGETMQIADNESSEFQQF
                     MDAVSAVERTFDFIVIDTPGSDSYLMRLAHSMADTLVTPINDSFLDFDVLGTVDPATY
                     SVTGESHYAEMVRDTRRKRRQLDGASTDWIVVRNRLSMLGSRNKQLVADGLKELSFRL
                     GFRSIDGFAERVVYREFFPRGLTALDDLDETTLGTRPSMGHVTAREEVTSLLRQLKLP
                     LDERGRRRAANRAEWFTQVDKPLEVHDIFSA"
     gene            2354..3205
                     /locus_tag="VGA15_24000"
     CDS             2354..3205
                     /locus_tag="VGA15_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9590790.1"
                     /translation="MKRGIAVLASLSALVTVAYFTASKWAIRHETLTFNDPARDNRPV
                     AVDIAVRWDKEMQANAGMIKLPVAILNHGNTVKFTEYSFLANVFAARGYLAVSIQHDL
                     PTDAPLVTKVGELYVGRLPQYQRGIANIRFAVDQMKKIEPNADYDRLTMVGHSNGGDI
                     SMYFAKMYPDEIKKVVTLDNLRVPFMTDGRFKILSFRSKDPVFKPDPGVIPNEEVCEK
                     SGITVVQTGYQHTDMSDRGPDNVKNTIQGMLDKFLDDDSPLKPVESNTPKITEPGPVA
                     LYAPVKN"
     gene            3336..4094
                     /locus_tag="VGA15_24005"
     CDS             3336..4094
                     /locus_tag="VGA15_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9590791.1"
                     /translation="MSGEPTKSKSLGEVSSAQTGKAGGLPEATPSASEDIAAFVAKAR
                     AMSPHAAGARGRLVFALDATMSRQPTWDMACALQADMFREAAALGSLDIRLVYYRGLN
                     ECRASGWISDSAQLAKLMGKIDCRGGNTQIGKVLSEARREAVASAVRAVVFVGDAMEE
                     RVDDLCAKAGELGLLKVPVFLFQEGHDDVAEQAFREIARLTGGAWCRFDPGAAAQLRE
                     LLRAAAAYAAGGREALLRLSKSGSGAAKLLGQMK"
     gene            complement(4215..4529)
                     /locus_tag="VGA15_24010"
     CDS             complement(4215..4529)
                     /locus_tag="VGA15_24010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590792.1"
                     /translation="MDDLDARLRHSVASQLNADVIDWQCPRLVDEAVDGVLGGLYPLS
                     NSERAALRRTMIRMLSEWKATTDWFIQVTDVDWTFDTETEQVLKGIVAKLSRSFEPYA
                     SP"
     gene            complement(4768..5169)
                     /locus_tag="VGA15_24015"
     CDS             complement(4768..5169)
                     /locus_tag="VGA15_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX9590793.1"
                     /translation="MAAQELTTIPSSFGPQDTMNRLEAAVRAKGMTVFARIDHAAGAT
                     GAGLSLRPTEVLIFGNAKAGTPLMQSVQTIGLDLPLKALVWQDASGKTWLSYNDPAWL
                     AQRHGLSGETEAAVNMMAGALEAVAKAATTG"
BASE COUNT         1061 a         1677 c         1625 g         1040 t
ORIGIN      
        1 cgatgttgtc gatcaggcgt gtctttccaa gcctggccgc aaccaggatt cgcactggcc
       61 cgtccttcgc cgaggcgatc ggcgcgaggg tatcggcgtg ccggggctcg taatagtcga
      121 ggacgaagcc ggcgttcgtg atcagttcag cgccgccggc catcgcagcc tcgatattat
      181 cgcccgcacg caaactcgag gcgctttcct tcatggtgcg atacagcgtc ggtgctatca
      241 gccgctcttc cggggacaaa tagacgttcc gcgaagacat cgcgaggcca tcgcgctcac
      301 gcaccgttcg cgagccgatc accctgacgc caaggtcgag atccccggcc atccgggtca
      361 cgacccgcag ttgctggaaa tccttttcgc cgaagatcgc gaaatccggc cggcactggg
      421 tgaacagctt gccgaccacg gtggtgacgc cgccgaagaa gtgcggccga aagcgatcct
      481 ccaggcccgc tgtcgccggc ccctcggtca atatccgggt ggcgaagccg tccgggtaca
      541 tggtcttgac gtccggattc cagatcaggt cgacattttc tgcggcgagc ctggcgacat
      601 cggcctccca ggtgcgcggg tacgaaccga aatcctccgt cggcgcgaat tgggtcgggt
      661 tgacgaagat ggaaacgata acccttttgg cgcgacgttt cgccagccga accagcgaca
      721 catggccgtc atggagcgct cccatggttg gcaccagcgc cgttgtgttc ttttttgctc
      781 tgatgctatc gagggcgcga cgcaacgcag ggaccgtgcg gacgatcatc gggcttcgcg
      841 acatctggac tcgatctgtg tggtcaagaa ggcggcggct tctgaccttg cggcgggatc
      901 gagaccttat caaagccaac gagtcgtcgc caagccgtca gtcgacgcgc gggctttcgg
      961 ccaggcttgg cgggcagccg ctagaggttg cggcgaatca gccgcgctac cacgtcgcgt
     1021 ctgcgatcaa tcattaaggc acacgacaaa tgtttcgaat gcgtgcttgc gcatttcact
     1081 tgaccgcggt cgcgcgtagt tgaagatatt cgcaggggtc aaatcatgtt gatgcaaacg
     1141 agtcagggcc agtcgggctc ggcgcatgtc gttgtgctgg gcaacgagaa gggcggttcg
     1201 ggtaaatcca ccaccgcctt gcacgttgct gtggccttgt tgaaagcagg ccagcgcgtc
     1261 gccaccatcg atctcgactg ccgccagcaa agttttaccc gctatatcaa caaccgacgc
     1321 gcttgggcgc gccgcaccgg cctcgatctg gaacttccgg tgcattgctg catcaagctc
     1381 ggcgagacca tgcagatcgc cgacaacgaa tcctccgagt tccagcaatt catggacgcc
     1441 gtgagcgcgg tcgagcggac tttcgatttc atcgttatcg atacccccgg gtccgatagt
     1501 tacctgatgc gtctggcgca ctcgatggcc gatacgctgg tgacgccgat caatgacagc
     1561 ttcctcgatt tcgacgtgct gggaaccgtc gaccccgcca cctattcggt gaccggcgaa
     1621 agccattatg cggagatggt gcgcgatacc aggcgcaagc gccgccagct cgatggcgcc
     1681 tctaccgact ggatcgtggt tcgcaaccga ctctcgatgc tgggatcgcg caacaagcag
     1741 ctggtcgccg acggtctcaa ggagctgtcg ttcaggctcg gcttccgctc gatcgacggt
     1801 tttgccgaac gcgttgttta ccgcgagttt tttccgcgcg ggctgaccgc gctcgacgac
     1861 ctcgacgaga ccacactcgg cacccgcccc agcatgggcc acgtaacggc gcgcgaggag
     1921 gtcacgagcc tgctgcgcca attaaaactg ccgctcgatg agcgaggccg ccgccgtgcc
     1981 gcgaaccggg ccgaatggtt cacgcaggtc gacaagccgc tcgaagtcca cgatatcttc
     2041 agcgcctaat ataagcctat ggtagcgggt tactacctgc ggtggaacga tcttcagtcg
     2101 gccacgtttt taccatgcct ttacctaacg cctgaaccaa accttgattc atgccatccc
     2161 agagtgagcg gggtggccga ccctcgtttg tgcatctgca caataagagg gtgcgtcgca
     2221 caacgatgag gcggtgactt cacaacattt actcttcctg tcacgcctgc gtgacatgta
     2281 tcatagataa gggaccacag agtcccgatc attggaacac aggcctccag ggtcgccgcg
     2341 ccgaggacga aatatgaaac gtggaattgc cgttcttgct tcgctttctg cgctggtaac
     2401 agtcgcgtat tttaccgcga gcaagtgggc gatccgccac gagactctga cgttcaacga
     2461 tccggcacgc gacaaccgcc cggttgcggt cgacatcgcc gtacggtggg acaaggaaat
     2521 gcaggcgaat gccggcatga ttaagctgcc ggtagcgatc ctcaatcatg gtaacaccgt
     2581 caagttcacc gagtactctt ttctggccaa cgtgtttgcc gcccgcggct atctggcggt
     2641 cagcattcag catgatcttc cgaccgatgc gccgctggta acgaaagtcg gcgaactcta
     2701 tgtcggtcgc ttgccgcagt atcagcgcgg cattgccaac atccgcttcg cggtcgacca
     2761 aatgaaaaag atcgaaccga acgcggatta tgatcgtctg accatggtcg gacattcgaa
     2821 cggcggtgac atctcgatgt atttcgccaa gatgtacccc gacgagatca agaaggtcgt
     2881 gacgctggat aatttgcggg tgccgttcat gaccgacggc agattcaaga tcctctcgtt
     2941 ccgttccaag gatccggtct tcaagcccga tccaggcgtc attcccaatg aggaggtctg
     3001 cgagaagtcc ggcatcacgg tcgtgcagac cggctaccag cacaccgata tgagcgaccg
     3061 tggtcctgat aacgtcaaga acacgatcca gggcatgctc gacaaattcc tcgatgacga
     3121 cagtccgctc aaaccggtcg agagcaacac gccgaagatc accgagccgg gacctgttgc
     3181 gctgtacgcg ccggtcaaga actgacggca ggtatcgcgc ccccggtcga aaaatcgcgt
     3241 cagcgtccga tctgaccgga atcagctccg ccgccacgca ttgaccgctc ccttgtcccg
     3301 gcccacataa gactcgctgc aatccgcgag tgcccatgtc cggcgaaccg accaagtcca
     3361 aatctttagg cgaagtgtct tcggcgcaaa ccggcaaagc cggcgggctg cctgaggcga
     3421 cgccgtcagc ttccgaagac attgccgctt ttgtcgcaaa ggcccgggcg atgtcgcccc
     3481 atgccgcagg cgcgaggggt cggctggtgt tcgcgcttga cgctaccatg agccggcagc
     3541 cgacatggga tatggcctgt gcgctgcagg ccgatatgtt ccgcgaggcg gcggcgctgg
     3601 gcagcctcga tatccggctt gtctactatc gcggcctcaa cgagtgccgc gcctcggggt
     3661 ggatttccga cagcgcgcaa ctggcaaagc tgatgggcaa gatcgattgc cgcggcggca
     3721 acacccagat cggcaaggtg ttgtcggaag cgcggcggga agccgtggcc tcggcggtgc
     3781 gcgcggtggt gttcgtcggc gacgccatgg aggaaagggt cgacgatctc tgcgccaagg
     3841 cgggcgaact cggcctgctc aaggtgccgg tgttcctgtt ccaggagggt catgacgacg
     3901 tcgccgaaca ggcgtttcgg gagatcgcgc gcctgaccgg tggcgcgtgg tgcaggttcg
     3961 atcccggcgc cgcggcgcaa ctgcgcgagc tgctgcgcgc agcggcggct tatgccgccg
     4021 gcggacgcga agcgctgctg cggttgtcga agagcgggag cggcgccgcc aaattgcttg
     4081 gccagatgaa gtgagctcga ttctggccca gcgctgcgtc tgagccctga tcaggcgcgc
     4141 acagttgccg agattgcctt cgcgacggcg cgtcgggccc ataacgcacc actcgttctt
     4201 gcctggtgtc gccctcaagg gctggcatag ggctcgaaac tccgcgacaa tttcgcgacg
     4261 attcccttca gcacttgctc tgtttccgta tcgaaggtcc aatcgacatc ggtgacctgg
     4321 atgaaccaat cggtggtcgc cttccactcg gataacatcc ggatcatcgt gcgcctgaga
     4381 gccgcccttt cggaattgga cagcggatat aaaccgccca aaacaccgtc caccgcttca
     4441 tcgaccagcc gcggacattg ccaatcgatg acatccgcat tcaactggct tgcgacgctg
     4501 tgcctcaatc gagcgtctaa gtcatccacg agaacctccg agtgcggggc gagaaaagca
     4561 gtgcataaac cacgtcatcg gtgcactccc ggcggagatc gcaattgtcg gcccgatacg
     4621 gctagaagtt cacgctttct tggggtccgg gtaatcgcag ccgcgagggc tcagctggga
     4681 atgatgatgg cgagccaacc ccgttctagt cgcgggggcc gcccggcctg ccaccgccgg
     4741 caaaaattga agcgggaagt cacccggtta ccccgtcgtt gcggctttcg ccacggcttc
     4801 cagtgcgcca gccatcatgt tgaccgctgc ctcggtttcg ccgctcagcc catgccgttg
     4861 cgcaagccag gccggatcat tgtatgaaag ccatgtcttg cccgatgcat cctgccaaac
     4921 cagcgccttc agcggcaagt ccagtccgat ggtctgaacc gattgcatca gaggggtgcc
     4981 ggctttggca ttgccgaaga tcaaaacctc ggtcggccgc agcgacagtc cagcgccggt
     5041 cgctccggcg gcatgatcga tgcgggcaaa caccgtcatg cccttggccc tcaccgcggc
     5101 ctcgagccgg ttcatggtat cttgcggccc gaaactgctc ggtatcgtcg tcaattcctg
     5161 cgccgccatt gcgcgctccc tggccgtccg gctgcggtca ggcgggagaa taataaattt
     5221 gtgctgataa cgccatcggc atcgaacagg ccttacttga tcaaccccgc gagcctcggc
     5281 agcgtcaggc tgatctcggg gatgcagatg atcaacagca gcccggcaaa cagcgccagc
     5341 atgtacggca cataggtgcg gaagtgctgc ccgacactca gatttgcaaa gcgcagcgcc
     5401 atc
//
LOCUS       DASWGM010000885         7903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1067295,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000885 DASWGM010000000
VERSION     DASWGM010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067295"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..651)
                     /locus_tag="VGA15_24020"
     CDS             complement(1..651)
                     /locus_tag="VGA15_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590794.1"
                     /translation="MAAGRAGRLRVALALAVAGALSGCGGGSMFGASSDSSPSISDRF
                     GQLFGSKSQAVGEAPPPAVDNELTCPSVSIRAGASTYAVAAPGKQAVGNDLRYQATIT
                     RTARDCNLNGGQITARIGIQGRVIAGPAGNPPTIEVPIRVAVVQSGVQEKTIATKVYR
                     TTVSMEEGSSVPFSLVAEDLVYPVPPGATGDSYVFYIGFDPQALKPEPKAATRKKK"
     gene            complement(812..1399)
                     /locus_tag="VGA15_24025"
     CDS             complement(812..1399)
                     /locus_tag="VGA15_24025"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX9590795.1"
                     /translation="MALFRLPSSGPAALAPRGNGLLLRAPQMSDFLQWAHLREYSREY
                     LTPWEPIWPSDDLTRSGFRRRLRRYAEDIAADRSYPFIVFRESDGAMIGGITLANVRR
                     GIVQAGTIGYWVGQPHAHRGYMTSALRVLLPSLFGELNLHRVEAACIPSNAPSIRVLE
                     KCGFNREGLARRYLCINGVWQDHLLFGLLHEDFRG"
     gene            complement(1523..2812)
                     /locus_tag="VGA15_24030"
     CDS             complement(1523..2812)
                     /locus_tag="VGA15_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX9590796.1"
                     /translation="MSVDVTKLPSGLTVITDTMPHLETAALGVWAGVGGRDEKPNEHG
                     ISHLLEHMAFKGTARRSSREIVEEIEAVGGDLNAGTSTETTAYYARVMKADVPLALDV
                     LSDILANPSFVPDELEREKSVIVQEIGAAQDTPDDVVFEHLNELCYPDQPMGRSLLGT
                     AKTLKGFNRDTLSGYLSTHYRGPDMVVAAAGAVDHKRVVEEVTQRFSSFDGVAAPKPQ
                     AAKFGKGGSRVVHRELEQAHLTLALEGVPQTDLSLFSLQVFTNTLGGGMSSRLFQEVR
                     EKRGLCYSIYTFHAPYSDTGFFGLYTGTDPGDAPEMMEVIVDVINDSVETLTEAEVAR
                     AKAQMKAGLLMALESCSSRAEQLARHVLAYGRPLTVEELVTRIDAVSVESTRNAARGL
                     LSRSRPAVVALGSGRGLDTAVAFAEGLTRSKAKTLLH"
     gene            complement(2809..4227)
                     /gene="thrC"
                     /locus_tag="VGA15_24035"
     CDS             complement(2809..4227)
                     /gene="thrC"
                     /locus_tag="VGA15_24035"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX9590797.1"
                     /translation="MTRYISTRGEAPALGFCDVMLTGLARDGGLYVPEAWPQLSLDTI
                     AGFFGRPYWEVAVDVIRPFVGGEISDADLGRMANEAYATFRHPAVVPLSQVSPNQFVL
                     ELFHGPTLAFKDVAMQLISRLMDHVLAQRAQRTTIVVATSGDTGGAAVDAFAGIENVD
                     LIVLFPHGRISDVQRRMMTTTGAANVHALAVEGTFDDCQAIVKGLFNHHSFRDAVSLS
                     GVNSINWARIVAQVVYYFTSAVALGSPARTVDFTVPTGNFGDIFAGYVAKRMGLPIRW
                     LRIAANVNDILPRTLKTGIYEVREVHASASPSMDIQVSSNFERLLFEASRRDAASVRR
                     LMASLKQSGRFVLPDPTLAAIREEFDAGRADETETAAAIRAAWREAGDLVDPHTAVAL
                     AVADRDTPDSKIPNIVLSTAHAAKFPDAVEAACGVRPQLPPWLEGLMSKPEHIRVMKN
                     DQVEVERFVLSVSRAAKQGVAG"
     gene            complement(4511..5287)
                     /locus_tag="VGA15_24040"
     CDS             complement(4511..5287)
                     /locus_tag="VGA15_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HEX9590798.1"
                     /translation="MGVQARRWRSVASFGVFTLAMVALFAGLGVWQLQRRVEKHALIA
                     ALTERLAGAPEALPSPAQWSALTSAHDEFRRVRFAATYQPLPDAMVYSSGSAVREDVS
                     GPGTWAFMPAALPSGETVVINAGFVQNTMQDRGQQDRAVARLITHEPETLTGYIRLPE
                     AAGVLTPPENTAKRLWFTRDHLAMARSLGWGEGGRQVAPFYIDLEQPVPASGIPTPGP
                     LEVHLKDDHLQYAITWFGLAGAVVIAFGVWLRGQGRASSS"
     gene            complement(5287..5676)
                     /locus_tag="VGA15_24045"
     CDS             complement(5287..5676)
                     /locus_tag="VGA15_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX9590799.1"
                     /translation="MQDQTLPTVAESAMRGLACKCPRCGRGKLYAGFLDLRPNCEACG
                     LDYTFIDSGDGPAVFIIMIAGAIVVGCALIVEVKYQPPFWLHAVLWLPLILATTLLPL
                     RSMKSLLIALQFHHKAAEGRLIDREPK"
     gene            complement(5871..6728)
                     /locus_tag="VGA15_24050"
     CDS             complement(5871..6728)
                     /locus_tag="VGA15_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX9590800.1"
                     /translation="MATAQLKHHDYHLVDPSPWPIVGAVSAFIMAVGAITWMHHLFAA
                     APIIFGIGTIGVLYTMAGWWTDVIREAQYKGDHTRVVQISHRYGMILFIASEVMFFVA
                     WFWAFFNSSLFPADPVHATREALFGGVWPPKGIETFDPWHLPLLNTLLLLTSGTTVTW
                     AHHALLENDRKGLKYGLILTVLLGAAFTCVQAYEYAHAAFSFAGNIYGATFFMATGFH
                     GFHVLVGTIFLIVCLARAYAGHFSPTQHLGFEFAAWYWHFVDVVWLFLFICIYVWGHG
                     AETMAHGAH"
     gene            complement(6814..7491)
                     /locus_tag="VGA15_24055"
     CDS             complement(6814..7491)
                     /locus_tag="VGA15_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX9590801.1"
                     /translation="MDKPPMPQVPIENPKTVSRRGPVLCGEPVTRRGLGRDAVVAASC
                     GLVVALMVGASYAAVPFYNWFCRTTGFNGTTQVATSAPSSAPLARKIAVRFDANVAPG
                     LPWKFEPEQTEIEIKIGEVVTVFYNVTNQSARTTIGQAAYNVAPLTVGAYFQKINCFC
                     FTEQTMAPGEKREMPVVFYVDPALVADSDNDGLNTITLSYTFYPVREPAPKPLAASEP
                     DKRRKDL"
     gene            complement(7497..7661)
                     /locus_tag="VGA15_24060"
     CDS             complement(7497..7661)
                     /locus_tag="VGA15_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoxF protein"
                     /protein_id="HEX9590802.1"
                     /translation="MDDKGKPDGIVLTEAQKKSRRERSIAIALALGVLVVLFFAVTIV
                     KGPAVLVRPM"
     gene            complement(7679..>7903)
                     /locus_tag="VGA15_24065"
     CDS             complement(7679..>7903)
                     /locus_tag="VGA15_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEX9590803.1"
                     /translation="PWPLGYFDAVYGLTSLVLGGGMLALAINVYRRREGSEALRATRK
                     LFAFSILYLFALFATLLLEVVAHAVAPSIW"
BASE COUNT         1524 a         2611 c         2448 g         1320 t
ORIGIN      
        1 ttacttcttc ttccgcgtcg cggccttcgg ctccggcttc agcgcctgcg gatcgaatcc
       61 gatatagaag acgtaggaat cgccggtcgc tcccggcggt accggataca ccagatcttc
      121 ggccaccaga ctgaacggca cgctgctgcc ctcctccatc gacacggtgg tccggtagac
      181 cttggtggcg atcgtcttct cctgcacgcc ggactgcacc accgcgacac gtatcggaac
      241 ctcgatggtc ggcggatttc ccgctgggcc agcaatgacg cggccctgaa taccgattcg
      301 cgcggtgatc tggccgccgt tcaggttaca atcgcgcgcg gtgcgggtga tggtcgcctg
      361 ataacgcaga tcgttgccga cggcctgctt gccgggcgcc gctaccgcat aggtggacgc
      421 gccggcgcga atgctcaccg aggggcaggt caattcattg tccaccgccg gtggcggcgc
      481 ctcaccgacg gcttgcgatt tcgagccgaa cagctggccg aaccggtcgc tgatcgatgg
      541 gctggaatcg gacgacgcgc cgaacatgct gccgccgccg cacccggaca aggcgcccgc
      601 aacggcgagc gccagcgcaa cgcgcagacg gcccgctcga ccggcagcca tcgtccgcat
      661 catcagcctg ttacgcatca tccggatatc ctctgggacg tatcgacgcc gttggcgcgc
      721 gtgaaatatg cgcgctttcg cgcgttataa cagcgcaatg ccggcgaacc caaggcgcca
      781 aaaaaggccg tgccacaatc tgaaaacaag gttagccgcg aaaatcctca tgcagcaggc
      841 cgaacagcaa atggtcctgc cagacgccat tgatgcagag atagcgtcgc gccaggcctt
      901 cgcggttaaa gccacatttc tccagcaccc ggatcgaggg cgcattggag ggaatgcagg
      961 cggcctcgac gcgatgaaga ttgagttccc cgaacaacga cggcaatagc acgcgcagcg
     1021 ccgacgtcat gtagccgcga tgggcatggg gctgcccgac ccagtatccg atggtgccgg
     1081 cctgcacgat gccgcggcgg acgttggcga gcgtgatacc gccgatcatc gccccgtccg
     1141 attcgcgaaa tacgatgaac ggataggatc tgtcggcggc gatgtcttcg gcatagcggc
     1201 gcaaccgtcg ccggaagccc gagcgcgtca gatcgtcgga cggccagatc ggctcccacg
     1261 gcgtgaggta ttcccggcta tattcgcgca aatgcgccca ttgcagaaaa tcggacatct
     1321 gcggcgcgcg cagcagcagg ccgttgccgc gcggcgcaag cgccgccggt ccgctggatg
     1381 gcaaacgaaa cagggccatg tctgtactcc cctgcccaaa tctgggcgtc ccaaagcacc
     1441 ctattgttcg agcatgatct tttcggaaaa ccggtgtcca ccctcgggtc atgcccaagg
     1501 gcatgctttt ccggatcatg ctctagtgaa gcagcgtctt ggccttcgat cgggtcaatc
     1561 cttccgcaaa agccaccgcc gtgtccagac ccctgccgct gccaagcgcg accaccgccg
     1621 gccggctgcg cgataacagt ccccgcgccg cgttgcgcgt tgattccacg ctgacggcgt
     1681 cgatcctggt caccagttcc tccaccgtca gcgggcgccc ataggccagg acatgccgcg
     1741 ccagctgttc ggcgcgcgaa ctgcagctct ccagcgccat caatagcccc gccttcatct
     1801 gcgccttggc gcgcgcgacc tcggcttccg tcagggtctc gacggagtcg ttgatgacat
     1861 cgacgatcac ctccatcatt tcaggcgcgt cgcccggatc ggtgccggta tagagcccga
     1921 aaaacccggt gtcggaataa ggcgcatgga aggtgtagat cgagtagcac agcccgcgct
     1981 tctcgcgcac ctcctggaac agccgcgacg acatcccgcc gcccaacgta ttggtaaaaa
     2041 cctgtaagga gaacaacgac aggtcggtct gcggcacccc ctcgagcgcc agcgtcagat
     2101 gcgcctgttc gagttcgcgg tgcaccacgc gggagccgcc cttgccgaac ttcgcggctt
     2161 gcggctttgg cgcagccacg ccgtcgaagc ttgagaaccg ctgcgtcact tcctcaacca
     2221 cccgcttgtg atcgacagca ccagccgccg ccaccaccat gtccgggccg cggtaatgcg
     2281 tcgaaaggta gccgctcagc gtatcgcggt taaaaccctt caaggtcttt gccgtgccga
     2341 gcaatgagcg tcccatcggc tgatccgggt aacacaattc gttgagatgt tcgaacacca
     2401 cgtcgtcggg cgtatcctgc gcagcaccga tctcctgcac gatgacgctc ttttcgcgct
     2461 ccagctcgtc gggcacgaac gacggattgg cgagaatgtc ggacagcacg tcgagcgcca
     2521 gcggcacgtc ggccttcatc accctcgcat aataggcggt ggtttcggtc gaggtgccgg
     2581 cgttgagatc gccgccgacc gcctcgattt cctcgacgat ctcgcgcgac gagcgtcgcg
     2641 cggtgccctt gaacgccatg tgttcgagca gatgcgagat gccgtgctcg ttcggctttt
     2701 cgtcgcggcc gccgacgccg gcccacaccc cgagcgccgc ggtctcgaga tgcggcatgg
     2761 tatcggtgat cacggtaagg ccggagggaa gcttggtgac atcgacgctc atccggcaac
     2821 tccttgcttc gcggcacggc tgaccgacag cacgaaccgc tcgacttcaa cctgatcgtt
     2881 cttcatgacc ctaatgtgct cgggtttgct catcagacct tcgagccagg gcggcagctg
     2941 cggacgcacg ccgcaggcag cctccaccgc atcgggaaat ttcgcggcat gcgcggtcga
     3001 cagcacgatg ttcggaatct tcgaatccgg ggtgtcgcga tcggccaccg ccagcgccac
     3061 cgcggtatgc ggatcgacga gatcgccggc ctcgcgccag gcggcacgga tcgcggccgc
     3121 ggtctcggtc tcgtcggcac gcccggcgtc gaattcctcg cggatcgccg ccaatgtcgg
     3181 gtcgggcagc acgaaacgcc cggactgttt cagcgacgcc atcagccggc gcacgctggc
     3241 cgcatcgcgg cggctcgcct cgaacagcag ccgctcgaaa ttcgaggaga cctgaatgtc
     3301 catcgacggc gacgccgacg cgtgaacctc gcgcacttca tagatgccgg tcttgagcgt
     3361 gcgcggcagg atatcgttga cgttggcggc gatccgcagc cacctgatcg gcagacccat
     3421 gcgcttggcg acatagcccg caaagatatc gccgaaattc ccggtcggca cggtgaaatc
     3481 caccgttcgc gcgggcgagc cgagcgcgac cgcggacgtg aaataataca ccacctgggc
     3541 gacgatccgc gcccagttga tggaattgac gcccgatagc gaaaccgcat cgcgaaagct
     3601 gtggtgattg aacaggccct tcacgatcgc ctggcaatcg tcaaacgtgc cttcgaccgc
     3661 aagcgcatga acgttggcag caccggtcgt cgtcatcatc cgccgctgca cgtcggagat
     3721 tcggccgtgc ggaaacagca caatcagatc gacattttcg attcccgcaa acgcatcgac
     3781 cgcggcgcca ccggtatcgc cggaagtcgc gaccacgatg gtggtgcgct gcgcgcgttg
     3841 cgccagcacg tgatccatca gccgcgagat cagctgcatc gccacgtcct tgaacgccag
     3901 cgtcggcccg tggaacagct ccagcacgaa ttgattgggg ctcacttggc tgagcggcac
     3961 caccgccgga tggcggaacg tggcataggc ctcattggcc atgcggccga gatcggcatc
     4021 tgaaatttcg ccgccgacaa acgggcggat cacgtcgacc gcgacttccc aatacggccg
     4081 gccgaaaaag cccgcgatgg tatcaagcga aagctgtggc caggcttcgg gcacgtagag
     4141 gccgccgtcg cgggcgagcc cggtcagcat cacgtcgcag aatcctaggg ccggggcctc
     4201 cccccgcgtc gaaatatacc gcgtcaaacc gccctccaaa ggccttggtg cccgccaagc
     4261 ctttgatatt attggattat ataactttgc cgatgacaaa gccgcgcgac accataaagc
     4321 gttttcgggc gaaggggaaa tcgggattct gccctcctgg cgagaccacg gtttcccggc
     4381 agcgtatttg cgctgtccgg tgcgccgcca gaggtgccga gcagcgaagg ccacgaacgg
     4441 cgacgacgta gcgaaggtga acggccgaac gcgtggcgtc cttcgaggct cgccgaagac
     4501 ggcgagcacc tcaggatgac gaggcccgcc cctgccctcg cagccacacc ccgaacgcga
     4561 tcaccaccgc gcccgccagg ccgaaccaag tgatggcgta ctgcaggtga tcgtccttga
     4621 gatggacctc gagcggaccg ggtgttggga tgccgcttgc aggcaccggc tgctccagat
     4681 cgatgtagaa cggcgcgacc tgtcggccgc cttcgcccca gccgagcgac cgcgccatcg
     4741 ccagatgatc ccgggtgaac cacagccgct ttgcggtatt ttccggcggt gtcagcacgc
     4801 cggcggcctc aggcaagcgg atatagccgg tcaacgtctc cggctcatgg gtgatcagcc
     4861 gcgccaccgc gcgatcctgc tggccgcgat cctgcatcgt gttctgcaca aaacccgcat
     4921 tgatcacgac ggtctcgccg cttggcaggg ccgcgggcat gaacgcccag gtgccgggac
     4981 cggaaacatc ctcacgcacg gcggagccgg aactgtagac catcgcatcc ggcaacggct
     5041 gataggtcgc ggcaaaacgg acgcggcgga attcgtcgtg tgccgaggtc agcgcgctcc
     5101 actgcgccgg cgacggcagc gcttccggtg cccccgcgag gcgctcggtc agggccgcga
     5161 tcagggcatg cttctcgacc cggcgctgca gctgccacac cccgagaccc gcgaacagcg
     5221 ccaccatcgc cagcgtgaac acgccaaagc tggcgacgct gcgccaccgt cgcgcctgca
     5281 ccccgatcat ttcggctcgc gatcgatcag ccggccttcc gcggccttgt gatggaactg
     5341 cagcgcgatc agcagcgatt tcatcgaccg cagcggcaac agcgtggtcg ccagtatcag
     5401 cggcagccac agcaccgcat gcagccagaa cggcggctgg tatttcacct ccacgatcag
     5461 cgcgcagccg accacgatgg cgccggcaat catgatgatg aaaaccgcgg ggccgtcgcc
     5521 ggaatcgatg aaggtataat cgaggccgca ggcttcacag ttcggccgca ggtcaaggaa
     5581 tccggcatac agtttgcccc tgccgcagcg cggacatttg caggcaaggc cgcgcatcgc
     5641 gctctcggcc accgtcggta aggtctggtc ttgcatgttc tgtccttccc ttctcccctt
     5701 gtgggagaag gtggcgcgga cgaagtccgc gccggatgag gggtcctttc gagttgagag
     5761 gcctacccct cacccgcctt cgctccgctc aggcaccctc tcccacaagg ggagagggaa
     5821 aaagaaaagg gcggccccgc ggccgcccct tgtagcacag gtgacaaaaa tcaatgcgcg
     5881 ccgtgggcca tggtttccgc gccgtggccc cagacataga tgcagatgaa caggaacagc
     5941 cagaccacgt cgacgaaatg ccagtaccag gcggcgaatt cgaagccgag atgctgggtc
     6001 gggctgaaat gcccggcata ggcgcgcgcc aggcacacga tcaggaagat ggtgccgacc
     6061 agcacatgga agccgtggaa gccggtcgcc atgaagaagg tcgcgccgta gatgttgccg
     6121 gcgaagctga aggcggcgtg ggcgtattca taggcctgca cgcaggtaaa cgccgcgccg
     6181 agcagcaccg tcaggatcaa accgtatttc aggcccttac gatcgttctc cagaagcgcg
     6241 tgatgcgccc aggtcaccgt ggtgcctgag gtcaagagca acagcgtatt caacagcggc
     6301 agatgccagg gatcgaaggt ctcgatgccc ttgggcggcc acacgccgcc gaacagcgcc
     6361 tcgcgggtgg cgtgcaccgg atcggcggga aacagcgacg aattgaagaa ggcccagaac
     6421 caggcgacga agaacatcac ttctgaggcg atgaacagga tcatgccata gcggtggctg
     6481 atctgcacca cgcgggtgtg atcgcccttg tactgcgcct cgcggatcac atccgtccac
     6541 cagcccgcca tggtgtagag cacgccgatg gtgccgatgc cgaaaatgat cggcgccgcc
     6601 gcgaacagat gatgcatcca ggtgatcgcg ccgacggcca tgatgaaggc cgagacggcg
     6661 ccgacgatcg gccacggact gggatcgacg aggtggtagt cgtggtgctt gagttgcgcc
     6721 gtagccattg cggtctctcc gttagcggtg ccgtgttatt cggcacgtat tcgtctcaaa
     6781 aatcaaatcc cgggatatcg atcatccctg cgatcagagg tctttcctgc gcttgtccgg
     6841 ctcgctcgcc gccagcggct ttggggccgg ctcgcgcacg gggtagaagg tataggacag
     6901 cgtgatggtg ttcagcccgt cattgtcgct gtcggcgacc agcgccggat cgacatagaa
     6961 caccaccggc atctcgcgct tttcgcccgg cgccatggtc tgttcggtga agcagaagca
     7021 gttgattttc tggaaatacg ccccgaccgt cagcggcgcc acattatagg cggcctgccc
     7081 gatcgtggtc cgcgccgact gattggtcac gttgtagaat acggtgacga cttcgccgat
     7141 cttgatctcg atttcggtct gctcgggctc gaacttccac ggcaatcccg gcgcgacatt
     7201 ggcatcgaaa cgcaccgcga tcttgcgcgc cagcggcgca gaggatggcg ccgaggtcgc
     7261 gacctgcgtg gtgccattaa agccggtggt acggcagaac cagttgtaga acggcaccgc
     7321 cgcataggac gcgccgacca tcagcgccac caccaggccg cagctcgcgg ccaccacggc
     7381 gtcgcggccg agcccgcggc gcgtgactgg ctcgccgcat aagactggcc cgcggcgcga
     7441 gaccgtcttc ggattctcga tcggcacttg cggcatcggc ggcttgtcca tgttgatcac
     7501 atcggccgca ctaggacggc cggccccttg acgatggtga ccgcgaaaaa cagcaccacg
     7561 agcacgccga gcgccagcgc gatggcgatc gaacgctcgc ggcggctttt cttctgcgcc
     7621 tcggtgagga cgattccatc tggcttgccc ttgtcatcca ttggctcgca tgcgccccct
     7681 accagatcga cggcgcgacg gcatgggcga cgacctcaag cagcaaggtc gcgaacagcg
     7741 cgaacagata aaggatcgag aacgcgaaca gttttcgcgt cgcgcgcagc gcttcgcttc
     7801 cctcacgacg gcggtagacg ttgatcgcca gcgccagcat gccgccgccg agaaccagcg
     7861 aggtcaggcc atagaccgcg tcgaaatagc cgagcggcca cgg
//
LOCUS       DASWGM010000886         8307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1608909,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000886 DASWGM010000000
VERSION     DASWGM010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608909"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..480)
                     /locus_tag="VGA15_24070"
     CDS             complement(172..480)
                     /locus_tag="VGA15_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9590804.1"
                     /translation="MAFPPTAQREMGYALFLAQMGERHATMAKTLGGFGGATVIEVRE
                     SHDGNAYRAVYTVRHADAVYVLHAFQKKSKKGIATPREEIDLIEKRLKDLIKEKEGRQ
                     "
     gene            689..970
                     /locus_tag="VGA15_24075"
     CDS             689..970
                     /locus_tag="VGA15_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9590805.1"
                     /translation="MIRSFRDQTTEAVFDGESPKGFPADLVKVARRKLRYLGAAGDLG
                     DLRSPPGDRLEALTGDRNGQHSIRINDQFRVCFVWTAEGPAEVEIVDCH"
     gene            985..1290
                     /locus_tag="VGA15_24080"
     CDS             985..1290
                     /locus_tag="VGA15_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX9590806.1"
                     /translation="MAKKLKPMHPGEVLREEFLVPLKMSAGALAKVCGLPRTRIERIA
                     SERTGITADTALRLAKALGTTAQLWLNLQNDYDVQIAKRDLGKTLDRIETVNKPKAA"
     gene            1307..1975
                     /locus_tag="VGA15_24085"
     CDS             1307..1975
                     /locus_tag="VGA15_24085"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX9590807.1"
                     /translation="MAGALIFDFDGVIADSEAIANTVLAETVTGLGHSTTLDQALARY
                     SGRRWDEAMAEIEAAIGKPLPSDFSSELKRATLDRFRTDLKEVSGAANFIRRFSHIPR
                     CIASSSSIDRLRLCLSVLALETEFGSQVFSADMVARGKPHPDIFLFAAGKLGVSPDEC
                     LVIEDSAGGIKAAVAAGMTAVGLCAASHIREGHDVKLRDAGALHLAHSWREVERFALQ
                     FFKC"
     gene            2088..3554
                     /gene="radA"
                     /locus_tag="VGA15_24090"
     CDS             2088..3554
                     /gene="radA"
                     /locus_tag="VGA15_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX9590808.1"
                     /translation="MAKNTLSFVCQNCGAAYNRWQGKCGSCGEWNTLAEEDTSGATTM
                     PVSIRSKRKGRLFALETLTGKSVEAPRLPSGMAELDRVTGGGFVRGSVLLVGGDPGIG
                     KSTLLTQATSLMARAGHRAVYISGEEAVAQVRLRAERLGLADAPVQLAAETSVEDIVS
                     TLSEGPVPRLIVIDSIQTMWTDTVESAPGTVTQVRASAQALIRFAKKSGAAIILVGHV
                     TKDGQIAGPRVVEHMVDAVLSFEGEGSQQFRILRAVKNRFGPTDEIGVFEMTGLGLRE
                     VSNPSELFLSERDLGSPGTAVFAGIEGTRPVLVELQALVAPTSLGTPRRAVVGWDPSR
                     LSMVLAVLEAHCGVKLSGHDVYLNVAGGLRIQEPAADLAAAAALVSSLVNAPLPTDAV
                     YFGEISLSGAVRPVAQTSARLKEAAKLGFGRAVLPESARGDVGGDAGLVLNTVGGLTS
                     LVADIAARGSPKGHREGNRESTAVEKNATPARFRRQES"
     gene            3717..4346
                     /locus_tag="VGA15_24095"
     CDS             3717..4346
                     /locus_tag="VGA15_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX9590809.1"
                     /translation="MPITILDLLLLAVMLISGLLAMVRGFMREILSIAAWGAAAVVTL
                     YAFSKLLPTAKTYFNNDTVASVVVVAGVFVGTLIVVSVVTVRISDMILDSRIGALDRT
                     LGFLFGLARGLLIVVVAFLFFSWLVPDKQRPDWVTGAKSRVVLQGTGDWLMSLLPDDP
                     ENTILKRFKKNKPDDEQTDTDPAAPASGDGYTKPARDSLKKLIEKPAAR"
     gene            4370..5914
                     /gene="purF"
                     /locus_tag="VGA15_24100"
     CDS             4370..5914
                     /gene="purF"
                     /locus_tag="VGA15_24100"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX9590810.1"
                     /translation="MDCQQSPSDEAAQYDLNLDPPPTPDLLDSDLQDSDLEGDTLREE
                     CGVFGIFGHPDAAAITALGLHALQHRGQEAAGIVSFDGARFHSERRLGLVGDTFSRRA
                     VIDRLPGTSAVGHVRYSTTGETILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELV
                     RNGAMMQSTTDTEVILHLVAQSKRNRFIDRFIEALRAIEGAYSLVSLTNKKLIGARDP
                     LGIRPLVLGELDGCPILASETCALDIIGAKYVRDVNPGEVVVFDEHGTQSHKPFPPMP
                     PRPCIFEYIYFSRPDSIVGGRSVYDVRKAMGAELARESHIEADVVVPVPDSGVPAALG
                     YSRESGLPFELGIIRNHYVGRTFIQPTQSVRELGVRMKHSANRAAIEGKRIVLIDDSL
                     VRGTTSKKIVKMMRDAGAREVHFRIASPPITHPDYYGIDTPDRTGLLAATHNLEEMRQ
                     LIGADTLAFLSVDGIYRAMGEPGRDPAHPKFTDHCFTGDYPTPLTDMTQREPQPRQLS
                     LLAEAS"
     gene            6072..6812
                     /locus_tag="VGA15_24105"
     CDS             6072..6812
                     /locus_tag="VGA15_24105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590811.1"
                     /translation="MTLPLASRIALVTGASRGIGYATARALARAGAHVVAVARTQGGL
                     EELDDEIRNDGGAATLVPLDLTDFDGIARLGAALHERHGRLDILVGNAGVAGPSSPLG
                     HIELKPWNDVMAVNVSANFQLIRCMEPLLKQSDAGRAVFVTSAASHMALAYLGPYSAS
                     KAALEALVRVWASETASTPIRVNLFSPGPIRTRMRASVFPGEDPLMLDTPEQAAEFIV
                     PMCAPDWTETGKFYDYKTRSLLSFRAPA"
     gene            7037..8170
                     /locus_tag="VGA15_24110"
     CDS             7037..8170
                     /locus_tag="VGA15_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590812.1"
                     /translation="MTPMQHRISRTASIFIAAAALIGTTALPRNAHAGEIPTFAVDAS
                     WPKPLPNNWILGQVGGITVDRQGHIWVIHRPRSLTDDEKGASLTPPRSKCCVSAPPVL
                     EFDADGNLLRSWGGPGEGYEWVGREHGIEVDERGFVWVGGNADNDNAILKFTLDGKFV
                     TQIGKIAPSKGSNDTTQLGKPAETAIDKAADEIYVADGYGNRRVIVFDATSGAYKRHW
                     GAYGHPPDDSKQPPYDPKAPVSQQFANPVHCVKIANDGLIYVCDRINDRIQVFRKDGT
                     FVKEWFYEKNTLGNGAVWDIAIWPDPKQTFLLSADGENNEIRVLKREDGTVVGSFGRN
                     GRNAGQFHWIHAMAVDAQGNVYTAEVDTGKRIQKFKLTSDALR"
BASE COUNT         1526 a         2656 c         2575 g         1550 t
ORIGIN      
        1 gctgagtggc tccaatccgc cgcgcggcgg cgcgttgggt caagccgaga gacgtgattg
       61 ccttgtccag ccgaaggacg agctcagcct ttagaaaatg ctcttcggca tcggggaatc
      121 cgagctgcag ccacacgttt ctcgtcgggt ccttggccgc aggtgttttt gtcattgtcg
      181 tccttccttt tccttgatca gatctttcag acgtttttcg atcaagtcga tttcttccct
      241 gggcgtcgcg atgcccttct ttgatttctt ctggaacgca tgaagcacgt agactgcgtc
      301 ggcgtggcgc accgtgtaaa ccgcccggta ggcgttgcca tcatggcttt ccctcacctc
      361 gatcacggtt gctccgccga agccgccaag cgtcttggcc atggttgcat ggcgttcgcc
      421 catttgcgcc aaaaacagcg catagcccat ctctcgctgt gcggtcgggg gaaacgccat
      481 aaagtcggtc ctcgaggctc cgcgccagat aagcggcctc ggctcgtcag cccttcgaac
      541 catgacccat tatgtgatat ttcacataat gggtcaagcg gcgcgagcat cgtcccgtgg
      601 acaatcgaat tatagagcgg gcagacgctg tatcattgat tgatacccgg actttgatag
      661 ctgtatcatt aaatgataca cattgcctgt gatcaggagc ttccgggacc agaccaccga
      721 agccgtcttc gacggcgaaa gtccgaaggg ctttccggcc gatctggtca aggtggcgcg
      781 ccggaaattg cgctacctcg gcgccgcggg tgatctcggc gacttgaggt cgcctccagg
      841 cgaccgtctt gaggctttga cgggcgatcg caacgggcaa cactcgatca ggatcaacga
      901 ccaattccgg gtgtgctttg tttggacggc ggaaggcccg gcggaggtcg agatcgtgga
      961 ctgccactag aagaggacgc gacaatggcc aagaaactca agcccatgca tcccggtgag
     1021 gttctgcggg aggagttcct ggttccgctg aaaatgtcgg cgggcgctct tgccaaggtt
     1081 tgcggcttgc ctcgcacacg gatcgaacgg atcgccagcg aacggaccgg cattaccgcc
     1141 gatacggcgc ttcggctcgc caaggcactc ggcacaacgg cacagctttg gctcaacctg
     1201 cagaacgact atgacgttca gatcgcgaag cgcgatctcg gcaagactct cgatcgcatc
     1261 gagaccgtca acaagccgaa ggcggcctaa ccggcagtgc ctgataatgg cgggtgcatt
     1321 gatttttgac ttcgacggcg tcatcgctga cagcgaggcg atcgcgaaca ccgttctggc
     1381 agaaactgta accggtctgg gtcattcaac cacacttgac caagcactcg ctcgctattc
     1441 cggtcgaagg tgggatgaag caatggccga gattgaagcg gctattggaa agccactgcc
     1501 ttccgatttt tccagcgaac tcaagcgcgc gacgttggac aggttccgaa cggatttgaa
     1561 ggaagtgagt ggggccgcaa atttcattcg gcgtttttcc cacatacccc gatgcatcgc
     1621 atcatccagt tcgattgatc gtctgcggct gtgcctttcg gtgctggccc tggaaacgga
     1681 gttcggcagt caggttttca gtgccgatat ggtggcgcga ggaaagccgc atcctgacat
     1741 ctttctgttt gccgccggca aattgggcgt aagcccggat gaatgtctcg tgatcgaaga
     1801 cagcgcgggt gggatcaagg cggccgttgc agcggggatg acggccgttg gcttgtgcgc
     1861 tgccagtcat attcgcgagg gccacgatgt taagctgcgc gatgcgggag ccctgcattt
     1921 ggcgcattcg tggcgggagg tcgaacgctt cgctcttcag ttcttcaaat gctaggttgt
     1981 gtgccgcgtc ccgagcgccg cgcacccgct gggatcatgc gcgcaggcgc gctttttcat
     2041 gctattgaac gcggcgccgt caccccaacc agcgactcac ccccttcatg gccaagaaca
     2101 cgctctcctt tgtctgccag aactgcggcg cggcgtataa ccgctggcag ggcaagtgcg
     2161 gatcctgcgg cgaatggaat acgctggccg aggaggacac gtcaggcgca accacgatgc
     2221 cggtctcgat ccgcagcaag cgcaagggcc ggctgttcgc gctggagacc ctcaccggca
     2281 aaagcgtcga agccccccgc ctgccctccg gcatggccga gctcgaccgc gtcaccggcg
     2341 gcggttttgt gcgcggctcg gtgctgctgg tcggcggcga tcccggcatc ggcaaatcga
     2401 cgttgctgac gcaggccacc agcctgatgg cgcgggccgg ccatcgcgcg gtgtacattt
     2461 caggcgaaga agcggtagcg caggtgcggc tgcgcgccga gcggcttggc cttgccgatg
     2521 ccccggtgca gctcgccgcg gaaactagcg tcgaggacat tgtttccacc ctgtccgagg
     2581 gcccggtgcc gcgcctgatc gtgatcgatt cgatccagac catgtggacc gacacggtcg
     2641 aatcagcacc tggcacggtg acgcaagtgc gcgcctcggc gcaggcgctc ataagatttg
     2701 ccaaaaaatc cggtgccgcc atcatcctgg tcggccatgt gacaaaggac ggccagatcg
     2761 ccgggccgcg cgtggtcgag cacatggtcg atgcggtgct gtcgttcgag ggcgaaggct
     2821 cgcagcaatt tcgtattctg cgcgcggtga agaaccggtt tgggccgacc gacgaaatcg
     2881 gcgtgttcga gatgacgggc ctcggcttgc gcgaggtctc caacccttcc gaattgttcc
     2941 tgtccgagcg cgatctcggc agtccgggca ccgcggtatt cgccggcatc gagggcaccc
     3001 gcccggtgct ggtggaattg caggcgctgg tggcgccgac ctcgctcggc accccgcgcc
     3061 gcgccgtggt gggctgggat ccgagccggc tctcgatggt gctggcggtg ctggaggccc
     3121 attgcggcgt caaactgtcc ggccatgacg tttatttgaa cgtggcggga ggtttgcgga
     3181 tccaggagcc cgcggccgac ctcgccgccg ccgcagcctt ggtctcctcg ctggtcaacg
     3241 cgccgctgcc caccgacgcg gtctatttcg gcgagatttc gctgtcgggc gcggtccggc
     3301 cggtggcgca aacctccgcg cggctgaagg aagccgccaa actcggattc ggccgcgccg
     3361 tgctgcccga atcggcgcgc ggggatgtcg gcggcgacgc cggcctcgtg ctcaataccg
     3421 tcggtggatt gaccagcctg gtcgccgata tcgccgcccg cggttccccc aaaggccatc
     3481 gagagggaaa ccgcgagagc acagcggtgg agaaaaatgc aacacctgcg agattccggc
     3541 gccaggagag ctagccggac gtgaccttac cttggcccgc cgctatacct cccgcgcgca
     3601 aggcggggat tgcacacggt ttccgacctt gcgggacatg ccatatcacc gtcacctagg
     3661 gcggcaccga cacgccgatt cgcggttttg ggcttacgag cggacctgac tagccgatgc
     3721 cgataacgat acttgatctc ctcctgctcg ccgtgatgct gatttccggc ctgctggcga
     3781 tggtgcgcgg cttcatgcgc gagatcctgt cgatcgcggc ctggggtgcg gcggcggtgg
     3841 tcacgcttta tgcattctcg aaactgttgc cgaccgctaa aacctatttc aataacgata
     3901 cggtcgcgag cgtggtggtg gtcgccgggg tgttcgtcgg caccctgatc gtggtttccg
     3961 tcgtcacggt gcggatttcc gacatgatcc tggattcccg gatcggggcg ctcgaccgca
     4021 ccctcgggtt cctgttcgga ctggcgaggg gtcttttgat cgtcgtcgtc gccttcctgt
     4081 tcttcagctg gctggttccg gacaagcagc ggccggattg ggtcaccggg gccaaatccc
     4141 gggtggtatt gcagggaact ggggattggc tgatgtcgct cttgccggat gaccctgaga
     4201 acaccatctt gaagagattc aagaagaata aaccagatga cgagcaaact gacaccgacc
     4261 cggcggcgcc ggctagtggt gatggctata cgaagcctgc ccgggacagc cttaaaaagc
     4321 tgatcgagaa acccgcggca cgatgagatc aggccaaaag agaggcgcga tggactgtca
     4381 acagagccct tccgatgaag ccgcacaata cgatctgaac ctcgacccac cacccacccc
     4441 ggacttgctg gattccgatc tgcaagattc tgaccttgaa ggcgacacgt tgcgcgagga
     4501 gtgcggcgtg ttcggcatct tcggccaccc cgatgccgcc gcgatcacag ccctcggatt
     4561 gcacgccctg cagcatcgcg gccaggaggc cgcgggcatc gtttccttcg atggagcccg
     4621 gtttcactca gaaaggcgcc tcggcctggt cggcgacacc ttttcccgcc gcgccgtgat
     4681 cgaccggctg ccgggcacat cagcggtcgg ccacgtccgc tattccacga ccggcgaaac
     4741 catcctgcgc aacgtgcaac cgctgttcgc cgagctcaat gccggcggct tcgcggtcgg
     4801 ccacaacggc aatctcacca acgggctgac cctgcgccgc gaactggtgc gcaacggcgc
     4861 catgatgcaa tccaccaccg acaccgaagt catcctgcac ctggtggcgc agtccaagcg
     4921 caaccgcttc atcgaccgct tcatcgaagc gctgcgcgcg atcgaaggcg cctactcgct
     4981 ggtgtcgctg accaacaaga agctgatcgg cgcccgcgat ccgctcggca ttcgcccgct
     5041 ggtgctgggc gaactcgacg gctgcccgat tctcgcgtcg gaaacctgcg cgctcgacat
     5101 catcggcgcc aaatacgtcc gcgacgtcaa tccgggtgag gtcgtcgtgt tcgacgagca
     5161 cggcacccag agccacaagc cgtttccgcc gatgccgccg cggccctgca tcttcgaata
     5221 catctatttc tcgcggccgg attcgatcgt cggcggccgt tcggtctatg acgtgcgcaa
     5281 ggcgatgggc gccgaactcg cccgcgaaag ccatatcgag gctgacgtgg tggtcccggt
     5341 gccggattcc ggcgtgccgg cggcgctcgg ctactccagg gaatccggcc tgccgttcga
     5401 actcggcatc atccgcaatc actatgtcgg ccgcaccttc atccagccga cgcaaagcgt
     5461 gcgtgaactc ggcgtgcgca tgaagcattc cgccaatcgc gcggcgatcg agggcaagcg
     5521 gatcgtgctg atcgacgatt cgctggtgcg cggcaccacc tcgaagaaga tcgtcaagat
     5581 gatgcgcgat gccggcgccc gcgaggtgca tttccgcatc gcctcgccgc cgatcacgca
     5641 tcctgattat tacggcatcg atacgcccga tcgcaccggc ttgctggcgg cgacgcataa
     5701 tctcgaggag atgcgccagc tgatcggcgc cgacacgctg gcgttcctgt cggtcgacgg
     5761 catctaccgc gccatgggcg agcccggccg cgatcccgcg catccgaaat tcaccgacca
     5821 ttgctttacc ggcgactatc cgaccccgct caccgacatg acgcagcgcg agccgcagcc
     5881 gcggcaattg tcgctgctgg cggaggcgag ctagcgacgc ccattgtggt cgtccccgcg
     5941 aaggcgggga cccatcaccc ctggcctccg ttgtccagcc aaagttagac acaagcgccc
     6001 tcccatatca taaccctcac ggcgtatggg tccccgcctt cgcggggacg accacggaga
     6061 gtgttaccgc gatgaccctt cccctcgcct cccgcatcgc cctcgtcacc ggcgcctcgc
     6121 gcggcatcgg ctatgccacc gcgcgcgcgc ttgcaagagc cggcgcgcat gtcgttgcgg
     6181 tggcgcgaac ccagggcggc cttgaagaac tcgacgacga gatccgcaac gacggcggag
     6241 ctgcgaccct ggtgccgctc gatctcaccg atttcgacgg catcgcgcgg ctgggtgctg
     6301 cgctgcatga gcgccacggc aggctcgaca ttctcgtcgg caatgccggc gtcgccggcc
     6361 cctcctcgcc gctcgggcat atcgaattaa aaccgtggaa cgatgtgatg gcggtcaacg
     6421 tatcagcgaa ttttcagttg atccgctgca tggagccgct gctaaaacag tccgatgccg
     6481 gccgcgcggt gttcgtcact tcggcggcct cccacatggc gctggcctat ctcggtccct
     6541 actccgcctc aaaagccgcg ctggaagcgc tggtgcgggt gtgggccagt gagacggcgt
     6601 cgacgcctat ccgcgtcaac ctgttcagtc ccggcccgat ccgcacccgc atgcgcgcca
     6661 gcgtatttcc cggcgaagac ccgttgatgc tcgacacgcc ggagcaggcc gcggaattca
     6721 tcgtgccgat gtgtgcgcct gactggaccg agaccggaaa attttacgac tacaagaccc
     6781 gctcgctgct gagttttcgc gcgccggcgt gagactccgt gtcccggacg cggtgcagcg
     6841 cccttcgcgc tgctccgcag agccgggacc cacaacgaca tcgcgaaagc atgggccccg
     6901 gatcagcagc gcaccgctac gcgctgcgca gcatccgggg cacgccggag caccgcccga
     6961 ttgactccca tgcctccccg ctattcactg cccgccaagc ggaaaaaacc cgcaaggaaa
     7021 aatacgggag gatgccatga cgccgatgca gcatcgcatt tccagaaccg cctccatctt
     7081 catcgccgcc gccgcgctga tcggcacgac cgcgctgcca cgcaacgctc atgccggcga
     7141 gattccgacc ttcgcggtcg atgcgtcctg gccaaagcca ttgcccaaca actggattct
     7201 cggccaggtt ggcggcatca ccgtcgaccg gcagggtcat atctgggtta tccaccgccc
     7261 gcgctcgctg accgacgacg agaagggtgc atcgctgacg ccgccgcgct cgaaatgctg
     7321 cgtgtcggcg ccgccggtgc tggagttcga cgccgacggc aatctcttgc gctcgtgggg
     7381 cgggcccggc gaaggctatg aatgggtcgg acgcgagcac ggcatcgaag tcgacgaacg
     7441 cggttttgtc tgggtcggcg gcaatgccga caacgacaac gcgatcctga aattcaccct
     7501 cgacggcaag ttcgtgacgc aaatcggcaa gatcgcgccg agcaagggca gcaacgacac
     7561 cacccagctc ggcaagcccg ccgagaccgc gatcgacaag gcggccgatg aaatctatgt
     7621 cgccgacggc tacggcaatc gccgcgtcat cgtgttcgat gccacgtcag gcgcctacaa
     7681 gcggcattgg ggcgcctacg gccatccgcc tgatgacagc aagcagccgc cttacgatcc
     7741 caaggcgccg gtgtcgcagc agttcgccaa tcccgtgcat tgcgtgaaaa tcgccaatga
     7801 tggcctcatc tatgtctgcg accgcatcaa cgaccgcatc caggtgtttc ggaaggacgg
     7861 caccttcgtg aaggaatggt tttatgagaa gaacacgctc ggcaacggcg cggtatggga
     7921 catcgcgatc tggcccgatc cgaagcagac ttttctgctc agcgccgatg gcgagaacaa
     7981 cgaaatccgc gtgctgaagc gcgaggatgg cacggtggtc ggcagttttg gccgcaacgg
     8041 acgtaacgcc gggcaattcc actggatcca cgccatggcg gtcgacgccc aaggcaacgt
     8101 ctataccgcc gaggtcgata ccggaaagcg catccagaaa ttcaaactga cctcggacgc
     8161 gctgcgctag cattacagtt gtttttttgc tttgaggtgc gcgcgctgag cggcagcctg
     8221 gtgagctcta cgccagagtg accatgcgat gatatgtgcg ggttggattc gctttcgagc
     8281 gagtctgatg gcgatgcggc ggacttc
//
LOCUS       DASWGM010000887         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_647548,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000887 DASWGM010000000
VERSION     DASWGM010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647548"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VGA15_24115"
     CDS             <1..262
                     /locus_tag="VGA15_24115"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX9590813.1"
                     /translation="LGYMARIEPEFDKRNVKIFGLSTDPVENHAKWATDIGVAPSYPV
                     IGDTDLTIAKACGTLPAVVSGGASKRTAAEALASTSQSKFGS"
     gene            689..820
                     /locus_tag="VGA15_24120"
     CDS             689..820
                     /locus_tag="VGA15_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590814.1"
                     /translation="MAYVLREIGVGVFGSITTAMIVVVIIAIATFGSNVRGKPLDVA"
     gene            complement(1045..>2351)
                     /locus_tag="VGA15_24125"
     CDS             complement(1045..>2351)
                     /locus_tag="VGA15_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9590815.1"
                     /translation="MLAEKVGRDTLLSQIVQMVAEAQRSRAPIQRLADQVSGWFVPVV
                     IVVALAAFGAWALFGPEPRMAFGLVAAVSVLIIACPCALGLATPISIMVGVGRGAEAG
                     VLIKNAEALERLERVDTLVVDKTGTLTEGKPRVVSIVPAGGFLEADILRLAASVERSS
                     EHPLADAIVRAAKERDLSLGQATDFDAPTGKGVTGKVEGKTVLLGNAAFLKSSGVETR
                     SLDAQAERLRGDGATVISIAIDGSLAGIFAIADPVKPSTPDALKALAAEGIKVIMLTG
                     DNRTTANAVARQLGIAEVEAEVLPDQKSAVVSKLQRAGHIVAMAGDGVNDAPALAAAE
                     VGIAMGTGTDVAMESAGVTLLKGDLGGIVRARRLSQATMRNIRQNLFFAFIFNAAGIP
                     IAAGVLYPTFGLLLSPIIAGAAMALSSLSVVGNALRLRSARL"
BASE COUNT          478 a          730 c          703 g          440 t
ORIGIN      
        1 acttggctac atggcgcgga tcgagccgga attcgacaag cgcaacgtca agatttttgg
       61 gctttccacg gatccggtcg agaaccacgc gaaatgggcg acagatatcg gtgtggcgcc
      121 gagctatccg gtgatcggag acaccgacct cacgatcgca aaagcctgcg gcacgctccc
      181 ggcagtggtt tcgggcggcg cgtccaagcg aacggccgcc gaggcgctcg cttcgacatc
      241 gcaatcgaag ttcgggtcgt gatggacacg caccggcact ttaggacgaa tagcggagga
      301 caagaccttc ccaaatcaaa cgacccgatc gttggctccg caacaaccgc aacgcgccca
      361 gacaaagtcg tacctcgtcc aacatgggaa tgatcagggc atctgctgca ttgcagcaat
      421 acgtcgattt acgcagtgcc acgccgagct cccgtcgcca tgacattcgt gtcggatcga
      481 gacacttggc ggctcccggt cgggcgcagc gcggtccggg ccgagaggcg gagtcatgtg
      541 tcgcgtcatt taatgaatga cggaccgcct gctcaaggat gcttcacgcc tatcagaccg
      601 aggtgtttcc gagttcgctc cgcgcgcgtg gcagcgggcc tcgtctattc gatgagccgg
      661 ctgagcgcga catccctcgc gcttcatctt ggcttatgtc cttcgtgaga taggcgtcgg
      721 cgtgttcggc tcgatcacca cggcgatgat cgtcgtcgtc atcatcgcga tcgcgacctt
      781 cggctctaac gtgcgcggca agccgcttga tgtagcgtag atttggaaat gtgcgcttcc
      841 ggtcaacagc ggtgtcacac ggcaacgtcc gttttcgaga gcgctatcat tcgggtcagg
      901 ctgctaagca cgaataatgg ggaaacagga gtcatccaac gacacgtccg cgtagaatgc
      961 cccaaatcca agctcggggt gccggatacg catgttcaga tgcgctgccg caggagcttg
     1021 agctagataa ttcgctgcat tgacctaaag ccgcgccgat cttaatcgca gagcgttacc
     1081 aacaacactc agcgacgaga gcgccatggc tgcgccggca ataattggcg agagtagcaa
     1141 tccgaatgtc ggatagagga cgcccgccgc aatggggatc cccgcagcgt taaagatgaa
     1201 tgcaaagaag agattctggc ggatattgcg catggtcgct tgggaaagac ggcgcgcgcg
     1261 cacgatgccg cccaaatcgc ccttcagcag cgtcacgccg gcgctttcca tcgcgacgtc
     1321 cgtacccgtg cccatcgcaa taccgacttc cgccgcagcg agagcgggag catcattgac
     1381 accatcgccg gccatggcga cgatatgccc ggccctctgc agcttactga caacagcgct
     1441 cttttgatcc ggaagaacct ctgcctcgac ttcggcaatg ccaagctgcc ttgcgacggc
     1501 gttcgccgtg gtccggttgt ctcccgtcag catgatgact ttgatgcctt ctgccgccag
     1561 cgccttcagg gcatccggcg ttgatggctt caccggatcg gcaatggcga agatgccggc
     1621 aaggctgccg tcgattgcga tgctgatgac agttgcacca tcgccgcgga gccgttcggc
     1681 ttgtgcgtcc aacgaccgcg tttcgacgcc tgatgacttc aggaaggcgg cgttgccaag
     1741 caggaccgtc ttgccttcga ctttaccggt cacgcccttg ccggtgggcg catcgaaatc
     1801 cgttgcctga cccaggctga gatcccgctc cttggccgca cgcacgatcg cgtcggcaag
     1861 ggggtgttcg ctcgatcgtt cgacgctggc ggccagccgc agaatatcag cttccagaaa
     1921 tccgcccgcg gggacgatcg agacgaccct cggcttgcct tcggtcagcg tgccggtctt
     1981 gtcaacgaca agcgtatcga ccctttccaa gcgctcaagg gcttcggcgt tcttgatcaa
     2041 gacgccagcc tcggcgccac ggccgacgcc gaccatgatc gatatcggcg tggcgaggcc
     2101 cagtgcacag ggacaggcaa tgatgagaac gctgacggct gcgaccagcc cgaatgccat
     2161 ccgcggttca ggtccaaaca gcgcccaggc gccaaaggcg gcaagcgcga cgacgatcac
     2221 gaccggtacg aaccagccgg aaacctgatc cgcaagccgt tgaatcggcg cgcgggatct
     2281 ttgtgcctcc gctaccatct gaacgatctg ggagagaagc gtgtcgcggc cgaccttttc
     2341 ggccagcatc a
//
LOCUS       DASWGM010000888         5985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_121349,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000888 DASWGM010000000
VERSION     DASWGM010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121349"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VGA15_24130"
     CDS             <1..452
                     /locus_tag="VGA15_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HEX9590816.1"
                     /translation="HGTPLGTADNDERYGAQGAFLIEAGISSSYHIANFFGLTEWIRQ
                     PEVVSTEAAKETAKETAKETAKEAAKEAAKQTAKQAVKQAVKPTPAVSSTPVNVPDLT
                     AVLWPLTTLNRRPESNRRPEPPRAPRRRGIDVGAVITRALTAAGLMK"
     gene            660..3149
                     /locus_tag="VGA15_24135"
     CDS             660..3149
                     /locus_tag="VGA15_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9590817.1"
                     /translation="MLKINGPTLADFLAGGGEMGALTRAYDWSASPLGLAETWPQSLR
                     TALRILLNTNHPMFIWWGEELIQFYNDAYRQTMGPERHPSALGQRGRECWAEIWDIIG
                     PQIEQVMSGGGATWHENQLVPVMRHGRLEQVYWTYGYSPIDEDDRVGGVLVVCRDVTR
                     DHLAAAVLREREAELARVQQIGRIGGFEVDLRTGFRNRRSPEYLLVHGLPPEAANETH
                     EDWVRRLHPEDRDATEKKFRDAIAGRVSDYNVQYRIIRPRDGETRWISVRSTIERDGQ
                     GRAIRLVGAHTDVTEQVMAEQALRQSEERYRRLADQLAGLTATLAQRVEEKTRERDRI
                     WNVSQDLLVVADRGGVWRTVNPAWTRTLGWSEAELLNRTSDWLEHPDDGGLTRAQFKK
                     LGTSGTTVRFESRFRHKDGSYRWLSWTGVSDEDANYAVARDVTAEKAAAERLKAAEEA
                     LLHSQKMEAVGQLTGGIAHDFNNLLTGIVGSLDLLQTRLDQGRTDNTARYINAAMTSA
                     NRAAALTHRLLAFARRQPLIPKSVDANQLVVSLEDLLRRTIGETIDLKIVAANDLWLT
                     LCDPNQLESALLNLAINARDAMPGGGKLTISTANVRLDGVTADTPALSPGDYICIGVT
                     DTGIGMSAEVAARAFDPFFTTKPIGQGTGLGLSMIYGFARQSNGHVTIDSKLGRGTSV
                     KLYLPRHHGDTASELASAIGAAEHGVTGETVLVVEDEPVVRAVILEMLGDQGYRTLEA
                     FDGPSGLRILRNPQRVDLLVTDVGLPGMNGRQLADQARETRPDLKILFITGYAESVAM
                     SDGFLQPGMEMITKPFDLDILSRRIRAMISG"
     gene            complement(3389..3691)
                     /locus_tag="VGA15_24140"
     CDS             complement(3389..3691)
                     /locus_tag="VGA15_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590818.1"
                     /translation="MKHFMITYRFANGTTEAWHREIQKFISALDNDPELKGKISYRCM
                     KNRDDSGYMHLAAAVDEQAIKTLQARDFFKHYTEKTRQVAGGEVVVTPIELIAETA"
     gene            complement(3718..4599)
                     /locus_tag="VGA15_24145"
     CDS             complement(3718..4599)
                     /locus_tag="VGA15_24145"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590819.1"
                     /translation="MILVTGATGLNGMELLRRLSAHGVAARALVRNPAKAEAIAALPH
                     VEIAQGDMAHPETLTVALHEINRAMLISSSAPDMLDVQSNFIDAARKAGVRHVVKLSG
                     IMPELHSAFRFARMHGEIEKRLEASEMAYTHLRAGEFMPAYFRQVPMIVGKGAMFLPM
                     EDARIASIDVGDIAEIAARVLTSSGHEGKTYPLTGPEALTMTEVAAKLSAATGKPIRY
                     VNVPPEDARQAQLAAGMPPYLADALFELFAERRNGKEGKVWPDAAALLGRPPTTFDEF
                     AGRNAAVFRGEAPPPKV"
     gene            4729..5106
                     /locus_tag="VGA15_24150"
     CDS             4729..5106
                     /locus_tag="VGA15_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9590820.1"
                     /translation="MAKTTSALTCGCPVAAFQKMISGKYKLRIVWDLQDGPKRYGEIR
                     TGLLRGSDGSSEIAPRVLSRELKALTEQGLIDRKDYGVVPPKVEYRLTRKGKSFVPVI
                     ASIRDWGTRHLAQVNTVEMAAAE"
     gene            complement(5171..5839)
                     /locus_tag="VGA15_24155"
     CDS             complement(5171..5839)
                     /locus_tag="VGA15_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9590821.1"
                     /translation="MANETQTKAKAGAVIVPVTLFEQNCTVIWCEATKKAVVIDPGGD
                     VPKILAAIKQTNVSVEKIWLTHGHIDHVGGAAELRDALKVPIEGPHIADKHLLDNVVT
                     SGARFGMTGVRDFAPDRWLDEGDQVSIGELKFDILHCPGHSPGSVVFFNKEMRFAHVG
                     DVLFNGSVGRTDLPGGSHATLIKSITDKLLPLGDDVGFICGHGAGSSIGQERLTNPFL
                     TGEM"
BASE COUNT         1122 a         1821 c         1939 g         1103 t
ORIGIN      
        1 cccacggcac cccgctcggg accgctgaca atgacgagcg atacggcgcc cagggcgcgt
       61 tcctgatcga ggcgggaatc tcgtcttcct atcacattgc gaatttcttt ggcctgaccg
      121 aatggattcg ccagcccgag gtggtttcga ccgaagctgc caaagaaact gcgaaggaaa
      181 ctgccaagga aactgccaag gaagctgcca aggaagctgc gaagcaaact gccaagcaag
      241 ccgtcaagca agccgtcaag ccaactccgg cagtttcatc gacgcccgta aatgtgccgg
      301 atctcaccgc ggtgctgtgg ccgctcacga cgctcaaccg ccgtcccgag tccaaccgcc
      361 gtcccgagcc accgcgcgcg ccgcggcgtc gcggtatcga cgtcggcgcc gtcatcaccc
      421 gcgcgctcac ggcggcgggc ctcatgaaat aacgggctta cggcggcgat cggtgccgca
      481 aggtcatggc gtgcgcgcgc gatggttaat gcggtgcgcg cgccgagacc tgcggttttg
      541 ttccatccgt tgaaaatata tttgggacca gatgccgtcc cctgagtttg ccggtggtgt
      601 gaccgggggt taagttccgg ctgccaattt gggctgaact gaccaaccga tcgtcttgaa
      661 tgttgaagat caacggaccg actcttgcgg atttcctcgc cggcggcggg gagatgggcg
      721 ccttgacgcg ggcctacgac tggtcggcaa gtccgctggg gcttgcggaa acctggccgc
      781 aaagtttgcg gaccgcgttg cgcatcctgc tgaataccaa ccatccgatg ttcatctggt
      841 ggggcgagga gctcattcag ttctataatg acgcctatcg ccagaccatg ggacctgagc
      901 gccatccaag cgcgctcggt caacgcggac gggagtgctg ggcggaaatc tgggacatta
      961 tcggtcccca gatcgagcag gtgatgagcg gcggcggtgc gacgtggcat gagaaccagc
     1021 tcgttccggt catgcgccac ggcaggctgg agcaggtcta ctggacctac ggctatagcc
     1081 cgatcgacga agacgatcgg gtcggcggcg tgctcgtcgt ctgccgcgac gtgaccaggg
     1141 accatcttgc cgcggcggtg ttgcgggaac gcgaagccga actggcgcgc gtgcaacaga
     1201 tcggccggat cggtggcttc gaggtcgacc tccgaaccgg ctttcgcaat cgccggtcgc
     1261 cggagtactt gctggttcac gggctgccgc cggaagcggc caacgaaacc cacgaagact
     1321 gggtgcgccg ccttcacccc gaggatcgcg acgccaccga aaagaaattc cgcgacgcca
     1381 tcgcaggcag ggtcagcgac tacaatgtgc aataccggat cattcgtccg cgcgacggcg
     1441 agacgcgctg gatttcggtc agatccacca tcgagcgcga cgggcagggg cgagccatcc
     1501 gcctggtcgg cgcccacacc gatgtcaccg agcaggtgat ggcggagcag gcgctgcggc
     1561 aaagcgaaga gcgataccgc aggcttgccg atcaactcgc cgggctgact gcgacgctgg
     1621 cgcagcgcgt cgaggaaaag acccgggagc gcgaccggat ctggaacgtt tcgcaggatc
     1681 tgttggtggt cgcggaccgg ggcggcgtct ggcgcacggt caatccggcc tggaccagaa
     1741 cgctcggctg gagcgaggcc gaattgctga accgcacctc ggactggctg gagcatcccg
     1801 acgatggcgg cctgacgcgc gcgcagttca aaaagcttgg caccagcgga accaccgtca
     1861 ggttcgagag ccgctttcgg cacaaggacg ggtcgtaccg ctggctgtca tggaccggcg
     1921 tgtccgacga ggacgccaac tacgcggttg cccgcgacgt caccgcggag aaggcggcgg
     1981 ccgaacggct caaggccgcc gaggaggcgt tgctgcactc ccagaagatg gaggcggtgg
     2041 ggcaattgac cggcggcatc gcccacgact tcaacaatct cctgactgga atcgtgggat
     2101 cgctggatct gctgcagacc cgtctcgatc aggggcggac cgacaacacc gcgcgctaca
     2161 tcaacgcggc gatgacatcg gcgaaccgcg ccgccgcgct gacgcatcgt ttgctggcct
     2221 tcgcgcggcg acaaccgctg atcccgaaaa gcgtcgacgc caatcagctc gtggtatcgc
     2281 tggaggattt gctgcgccgg acgatcggcg agaccatcga tcttaagatc gtcgcggcca
     2341 acgatctgtg gttgacgttg tgcgatccca atcagctgga aagcgcactg ctcaatctcg
     2401 ccatcaacgc gcgcgacgcc atgcccggcg gtggcaagct caccatctca accgcaaacg
     2461 tgcggctcga tggggtgacc gcggataccc ccgcgctttc gcctggcgat tatatctgca
     2521 tcggcgtcac cgacaccggg atcggcatga gcgcggaggt ggcggcgcgc gccttcgatc
     2581 cgttctttac caccaagccg atcgggcaag gtaccggact tgggctttcg atgatctatg
     2641 gatttgcgcg gcagtcgaac ggtcatgtga cgatcgacag caaattgggg cgggggacat
     2701 cggtcaaact ttatttgccg cgccatcatg gcgacaccgc aagcgagctg gcatcggcga
     2761 tcggggcggc cgagcacggc gtgaccggcg aaaccgtgct ggtggtcgag gacgaaccgg
     2821 tggtgcgcgc cgtcattctg gagatgcttg gagatcaggg atatcgaacg ctggaagcgt
     2881 tcgatggacc gtcggggctg cgcatcctgc gcaaccctca gcgggttgat cttttggtca
     2941 ccgacgtcgg cttgcccggc atgaacggcc gccagctcgc cgatcaggcg cgggaaacgc
     3001 ggccggatct caaaattctc ttcatcaccg gctacgccga gagcgttgcg atgtcggacg
     3061 gctttctgca gcccggcatg gagatgatca ccaagccgtt cgacctcgac attctctcac
     3121 ggcggattcg cgcgatgatt tcgggttagg gctgtaggtt tcttcccttc tccccttgtg
     3181 ggagaaggtg gcataggcgg ccttcggccg ccgttcttta ataggacgcc gatgctttgg
     3241 atcggctatg cgaagtccgt gccggatgag gggttgcagc gctcatcgat agaccgtgac
     3301 ccctcacccg tcgcggtgct cactgcgttc gcatcgcgcc accctctccc acaaggggag
     3361 aggggaagaa agaacgactc tctgatcgct acgccgtttc cgcgatcagt tcgatcggcg
     3421 tgaccacgac ctcgccgccg gcgacctgcc tcgtcttttc cgtgtagtgc ttgaagaaat
     3481 cccgcgcctg cagggtcttg atggcctgct cgtcgactgc cgcggcgaga tgcatgtagc
     3541 cggaatcgtc gcggttcttc atgcagcggt agctgatctt tcctttcagt tcgggatcgt
     3601 tgtcgagggc ggaaatgaat ttctggattt cccgatgcca ggcctccgtg gtgccgttgg
     3661 caaaccgata tgtgatcatg aagtgtttca tcgatggctc cttcagcggc ggagatgtca
     3721 aactttcggc ggcggcgctt caccccggaa cacggcggcg ttgcgcccgg cgaattcgtc
     3781 gaacgtcgtc ggcggtcgcc cgagcagcgc cgctgcatcc ggccagacct tgccctcctt
     3841 gccgttgcgc cgctcggcga acaactcgaa caatgcatcg gcgaggtaag gcggcatgcc
     3901 cgcggcaagc tgggcctgcc tcgcatcctc cggcggcaca ttgacatagc gaatgggttt
     3961 tccggtggcg gccgagagtt ttgcggcgac ctccgtcatg gtcagcgcct ccggaccggt
     4021 gaggggatag gtcttgcctt catgcccgga actggtcagc acgcgggccg cgatttcggc
     4081 gatatcgccg acatcgatcg acgcgatcct ggcgtcctcc atcgggagga acatcgcacc
     4141 cttgccgacg atcatcggga cctggcgaaa ataggccggc atgaactcgc cggcccgcag
     4201 atgggtgtag gccatttcgg aggcttccag ccgcttctcg atctcgccat gcatccgggc
     4261 gaagcgaaac gccgagtgaa gctccggcat gatgccggat agtttgacga cgtgccgcac
     4321 gcccgccttg cgcgcggcgt cgatgaagtt cgactggaca tcgagcatgt cgggggccga
     4381 ggacgaaatc agcatcgcgc gattgatctc gtgcaaggcc accgtgagcg tttcggggtg
     4441 cgccatgtcg ccctgcgcga tctcgacatg cggcagtgct gcgatcgcct cggccttggc
     4501 cggattgcgc accagcgcgc gcgcggcaac accatgcgcc gacagccggc gcagcaattc
     4561 cattccgttc agtccagtgg cgcctgtcac gaggatcatg gtcgggtctc cggttgcaca
     4621 cccaatctgg cgctctcatg ggcgataacg caagtatgga caaacatgat actatggatt
     4681 ttgtttgggg tgccggtatc ctgaaaacgc agtgttggag gcgacgatat ggcgaagaca
     4741 acatcagcgc tgacgtgcgg atgcccggtg gcggcgttcc agaaaatgat cagcggcaaa
     4801 tacaagctgc ggatcgtctg ggatctgcag gatggcccca agcgctatgg cgagatcaga
     4861 accggtctgc tgcgcggttc ggatggcagt tcggaaatcg cgccccgcgt gctcagccgg
     4921 gaattaaagg cgctcaccga gcagggcctg atcgaccgca aggattacgg cgtcgttccg
     4981 cccaaggtcg aatatcggct gacgcgcaag ggtaaaagct tcgttccggt cattgccagc
     5041 attcgcgact ggggcacgcg ccacctcgcg caggtgaaca cggttgaaat ggccgccgcc
     5101 gaatagcagc catcgcggga tcaaaaacct cgcggcaaat atgctgaggc cagtgacggc
     5161 acgccgggtc tcacatctcg ccggtgagga atggattggt cagccgctcc tggccgatgc
     5221 tggagccggc gccgtggccg cagatgaagc cgacatcgtc gccgagcggc aagagcttgt
     5281 cggtaatcga cttgatcagc gtggcgtggc tgccgccggg gaggtcggtg cggccaaccg
     5341 agccgttgaa caacacgtcg ccgacatggg caaagcgcat ttccttgttg aaaaaaacca
     5401 cgctgccggg cgaatgaccg gggcagtgca ggatgtcgaa tttcaactcg ccgatcgaaa
     5461 cctgatcgcc ctcgtcgagc cagcgatccg gcgcgaaatc gcgcacgccg gtcatgccga
     5521 accgcgcgcc gctggtgacg acgttgtcga gcagatgttt atcggcgatg tgcgggcctt
     5581 cgatgggcac cttcagcgcg tcgcgcagtt cggcggcgcc gccgacatgg tcgatatggc
     5641 catgggtcag ccagatcttt tcgaccgaca cattggtctg tttgatcgcg gccagaattt
     5701 tcgggacgtc gccgccgggg tcgatcacca cggccttttt ggtggcttcg caccagatca
     5761 ccgtgcagtt ctgctcaaac agcgtaaccg ggacgatgac cgcgccggcc tttgccttgg
     5821 tttgggtttc gtttgccatc gccgcacaat gccgattttt tgcggctcgc caaggggaaa
     5881 ttgggctgaa aaagtgggcg gttgacgctc ccgtgtcatc tcttcctggt tcaaactgaa
     5941 ccggctagcg gttccaggcg ttgcctcgcg cggcgcaatc aaaga
//
LOCUS       DASWGM010000889         4193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1906361,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000889 DASWGM010000000
VERSION     DASWGM010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906361"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VGA15_24160"
     CDS             complement(<1..327)
                     /locus_tag="VGA15_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX9590822.1"
                     /translation="MTINKRHDEFHTLDMSKGWETPTGYPAGIQQNILSGALDETNKR
                     GSRTRLLRFAPGVYTTTPFVHEYWEEVFLFSGDLTVGNDEHGKGGESFKPNTYACRPP
                     GAFHGPF"
     gene            complement(339..1847)
                     /locus_tag="VGA15_24165"
     CDS             complement(339..1847)
                     /locus_tag="VGA15_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9590823.1"
                     /translation="MIKSGEQHIASLRDGREIYLDGALVDDVTTHPAYRGAVASIGRM
                     FDFQTVPENRELMTFETETGTRASRIWQLPTSYDELVTRRKALEAWTELHAGFMGRAP
                     DHVASCISGMFMGSEVFEAYDAARAKALADYYRYARDNDLYLTYVIINPQADRSRSAA
                     AQSDPFLSAGLVDRDEDGITVRGAKMLATGGMMANEVFVTCIQPLQPGDEKYAISFAV
                     PMNAKGLKILSRKSYEAAAPSVFDNPLASRFDENDAVLYFDDVKVPWERVFIVDSIEM
                     CQKQFHATPAHVYQNYQAMIRLSIKLRFLLGIAHRTAETNCITGFPQVRETLGQLSAE
                     ASMVDAFVVAMETKGQQRGAYFVPDRRMLYAAQTLTQQLYAKVINTLRELAGGGMIML
                     PSSVKDFSNPEVARLIGKTQQSPAAGAIDKVKFYKLAWDAVGSEFASRHAQYEMFYAG
                     ATFVTKGHAFRTYDWNAATSLLDRMLSSYDLADEVAGKAGRLPRPERSPNHP"
     gene            complement(1768..2193)
                     /locus_tag="VGA15_24170"
     CDS             complement(1768..2193)
                     /locus_tag="VGA15_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590824.1"
                     /translation="MFVPASRRSLVRTGFLYNARTDGRKASLGIPALRTDAALACPDA
                     PLARPDAALARQATTMPVEIGFDMRESIRAGSRPIAFIKANSRAGSFVIEIASSSDAL
                     LNFRQSTKLIEGCDDQKWRTAYCELARWPRDLSRWRACR"
     gene            2192..3919
                     /locus_tag="VGA15_24175"
     CDS             2192..3919
                     /locus_tag="VGA15_24175"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX9590825.1"
                     /translation="MTAAGSNGAAAAPTHIETDVLVIGAGGAGMYAALEAARAGAAVV
                     LADRSLIGRGGATVMAQMTVAAALGEQTPDHWEHHLSDTLAAGRGLCDERLAALLCED
                     GPRRLREMDHWKVGWAREDGHIKQAQAPGHDRPRCAYVDFLATGPAVSRTLRSQLNAA
                     NVVRRIGELVIVDIAVRDGEACGATALHLPTGHAVTLAAKAVVIATGGLTGLYRRNSA
                     SSNMGGDGYALALRAGAELIDMEFVQFFPIGHLAPRLVGMDPIMWDPFRYKLGGKLLN
                     ADMREFEQDYATRDNRSDGRYVLTRDLATYAITREVEAGRGSPAGGAYLSFQHVPEAE
                     IRSAFGPVVDRLAANGIDLAKRPVEVAPIAHYHMGGVRVDETLQARVLGLYACGEAVG
                     GANGANRLSGNAITEAFVFGARAGRNAARRTLQGSSLWSPQAAGPALDLLRSAQRRDA
                     PNLAAVVVELKALMADTVGPFRTEEKLRSAVQAIERLKTEIGEVPFSSAAAFDPVLVD
                     WLDLRNMLLVAQSVTLPALARTESRGAHQREDHPGLDDNWRVNQLVALSRGKVELSRS
                     APSTGRAAA"
     gene            3916..>4193
                     /locus_tag="VGA15_24180"
     CDS             3916..>4193
                     /locus_tag="VGA15_24180"
                     /inference="COORDINATES: protein motif:HMM:NF024485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9590826.1"
                     /translation="MKATLAIQRGALGEPPRIESFEVTFEHGQSVLDGLRALRCEVDP
                     SLAFRFSCINANACKECMMLIDGRVDYACTVRLKEGLTALAPLPKKAL"
BASE COUNT          770 a         1295 c         1318 g          810 t
ORIGIN      
        1 gaacggaccg tgaaacgcgc ccggcggccg gcaggcatag gtgttcggct tgaatgattc
       61 accgcccttg ccatgctcat cgtttccgac cgtcaggtcg ccggagaaca gaaacacttc
      121 ttcccaatat tcgtgaacga acggcgttgt cgtgtagacg cctggcgcga agcgcaacag
      181 ccgggtccga ctgccgcgct tgttggtctc gtcgagcgct cccgacagga tgttctgctg
      241 aatacccgcg ggatacccgg tcggagtttc ccatcccttc gacatgtcga gggtgtggaa
      301 ttcatcatgg cgcttgttga tggtcatttg gaattctcct agggatggtt aggcgaccgc
      361 tcgggccggg gaagtcggcc ggcctttccg gcgacctcgt cggccagatc ataactggac
      421 agcatccggt cgagcaacga cgttgctgca ttccagtcat aggtgcggaa ggcatgtccc
      481 ttggtcacaa aggtcgcacc agcatagaac atttcgtact gcgcatggcg tgacgcgaat
      541 tcggagccga ccgcgtccca cgccaatttg tagaacttca ccttgtcgat cgcgcccgcc
      601 gcgggcgact gctgggtctt tccaatcagt cgcgcgacct ccggattgct gaaatccttc
      661 accgatgatg gcaacatgat cattccgccg cccgccaact cgcgcaatgt gttgatgacc
      721 ttggcgtaga gttgctgcgt cagggtctgt gcagcataga gcattcgtcg gtcaggcacg
      781 aaataagcac cgcgttgctg ccccttggtc tccatcgcca cgacaaacgc atcgaccatg
      841 ctggcttcgg cagacaattg gcccaacgtc tcgcgcacct gcggaaatcc ggtgatgcag
      901 ttggtttcag ccgtgcggtg ggcgatgccg agcagaaagc gaagcttgat cgagagccgg
      961 atcatggcct gatagttctg atagacgtgg gcgggcgtcg catgaaactg cttttgacac
     1021 atctcgatgc tgtcgacgat gaacacccgc tcccagggca ccttgacatc gtcgaaatac
     1081 agcacggcgt cgttctcatc aaaccggctc gccaacggat tgtcgaatac cgacggcgcg
     1141 gcagcctcgt aggattttcg cgacagtatc ttaagaccct tggcgttcat tgggacggcg
     1201 aatgagatgg cgtatttctc gtctcccggc tgtagcggct ggatacaggt cacgaagact
     1261 tcgtttgcca tcatgccgcc ggtcgcgagc attttcgctc cccgcaccgt aatgccatcc
     1321 tcatcgcgat cgactaatcc ggccgacaga aacggatcgc tttgcgcagc ggcgctcctg
     1381 gaacgatcgg cctgcggatt gatgatcaca taagtgaggt aaaggtcgtt gtcccgcgcg
     1441 taacggtaat agtccgcaag cgccttggcg cgtgctgcat cgtaagcttc gaaaacctcg
     1501 ctgcccataa acatcccgga gatgcaggat gcgacgtgat cgggagcccg tcccatgaag
     1561 cccgcatgca attcggtcca cgcctcgaga gctttgcggc gggtcacaag ttcgtcgtag
     1621 ctcgtgggaa gttgccagat tcgactggcc cgcgtacccg tctcggtctc gaatgtcatc
     1681 aattcacggt tttcgggaac agtttgaaaa tcgaacatcc gtccgatcga ggcaaccgcg
     1741 ccgcgatagg cgggatgggt cgtgacgtca tcgacaagcg cgccatcgag ataaatctcg
     1801 cggccatcgc gcaagctcgc aatatgctgt tcgccacttt tgatcatcac atccctcgat
     1861 cagctttgtt gattgacgaa agttcagcag tgcatcggaa gacgaagcta tttcaatcac
     1921 aaaagatccg gcacgtgaat tggccttgat gaacgcgatc ggtctgctgc ctgccctgat
     1981 ggattcgcgc atgtcaaagc cgatctcaac tggcatggtc gttgcttgac gcgccagcgc
     2041 cgcatcggga cgcgccaacg gcgcatcggg acacgccaac gccgcatcgg ttcgaagcgc
     2101 tgggatgccc agactggcct tccgcccatc ggtgcgcgca ttgtaaagga agccggtacg
     2161 aacgagtgac cgacgtgaag ctggaacgaa catgactgct gccggaagca acggtgccgc
     2221 ggcggcgccg acacatatcg agaccgacgt cctcgtgatc ggtgccggcg gcgccggcat
     2281 gtatgcggct cttgaagccg cgcgggccgg cgcggccgtt gttctggcgg accgcagcct
     2341 gatcgggcgc ggtggcgcca cggtcatggc gcaaatgacg gtcgccgcag cgctgggcga
     2401 gcagacgccg gatcattggg agcatcacct gtccgatacg ctcgcggccg ggcgcggtct
     2461 ttgcgacgag cgccttgccg cactgctgtg cgaggacgga ccgcggcggc tgcgcgagat
     2521 ggatcactgg aaggtcggct gggcgcgcga ggacggccat atcaagcagg cgcaggcgcc
     2581 cggccacgat cgaccccgtt gcgcctatgt tgattttctc gccaccggtc ccgcggtatc
     2641 gcgcaccctg cgctcgcagc tgaacgccgc gaacgttgtc cgccgcatcg gcgagcttgt
     2701 gatcgttgat atcgcggttc gtgatggcga agcctgcggc gcgacggccc tgcatctgcc
     2761 gactggacac gccgtcacgc tcgccgccaa ggccgtggtc attgcgaccg gcgggctgac
     2821 gggactttat cgccgcaaca gcgcgtcttc caacatgggc ggcgacggct atgcgctggc
     2881 tctgcgcgcc ggcgccgagc tcatcgacat ggagttcgtg cagttctttc ccattggcca
     2941 ccttgcgcca agacttgtcg gcatggaccc gatcatgtgg gacccgtttc gctacaagct
     3001 gggcggcaag ctgctcaatg ccgacatgcg tgaattcgag caggactatg cgacgcggga
     3061 caaccgcagt gacggccgct acgtgctgac gcgcgatctc gcgacctacg ctatcaccag
     3121 ggaggtcgag gccggccgcg gcagtccggc tggaggcgcc tatctgagct tccagcatgt
     3181 gcccgaggcc gagatccgca gcgccttcgg gccagtggtg gaccggcttg ccgccaatgg
     3241 catcgatctt gccaagcgcc ccgtggaagt cgcgccgatc gcgcattatc acatgggcgg
     3301 cgtgcgggtc gacgagacgc tgcaagcacg tgtgctcggt ctttacgcgt gtggcgaagc
     3361 ggtcggcgga gccaacggcg ccaaccggct atcgggcaac gcgatcaccg aagcctttgt
     3421 gttcggcgcc cgcgccggtc gcaatgctgc gcggcgcacc ttgcagggct cgtcgctctg
     3481 gtcgccgcag gccgctggtc ccgcactcga tctgctgcgc agcgcgcaaa ggcgcgacgc
     3541 ccccaatctg gcggcggtcg ttgtagaatt gaaggcgctg atggccgata cggtcgggcc
     3601 gttccgcacc gaggagaagc tgcgttcggc cgtgcaggcc attgagcgat tgaaaaccga
     3661 aatcggcgag gtcccgtttt cgtccgcagc ggcgttcgat cctgtgctgg tcgactggct
     3721 ggacctgcgc aacatgctgc tggtcgcgca atcggtcact ttgccggcgc tcgcccgcac
     3781 cgagagccgc ggcgcgcatc aacgcgagga tcatccgggt ctcgatgaca actggcgcgt
     3841 caatcagctc gtagcgcttt cgcgcggcaa ggtcgaactc agccgcagcg cgccatcgac
     3901 cgggagggcc gcggcgtgaa ggcaacgctc gccatccagc gcggcgcgct cggcgaaccg
     3961 ccacgcatcg aaagcttcga ggttacgttc gagcatggcc agtccgtgct cgacgggttg
     4021 cgcgcgcttc ggtgcgaggt cgatccgtcg cttgcgtttc ggttctcctg cattaacgcc
     4081 aacgcctgca aggaatgcat gatgctgatc gacggcaggg tcgattacgc ctgcacggtc
     4141 cgactgaagg aaggcctgac tgcccttgcg ccgttgccga agaaagcctt gat
//
LOCUS       DASWGM010000890         7758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1471400,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000890 DASWGM010000000
VERSION     DASWGM010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471400"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="VGA15_24185"
     CDS             <1..1953
                     /locus_tag="VGA15_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9590827.1"
                     /translation="RLWTEEGMPLSEATLHGAAEIAVPTLVSTLAISCVFTSVVFLDG
                     PAKYLFTPLGLAVVFAMLASYGLSRTLTPITIGLLLRSERRHTDNDGAPAGLASRLSA
                     AFDRSFERLRDGYAKFLTILLRRRAIVPLVAVLMLSLGAVMLVLVGRDFFPLIDGGQI
                     QLHVRAPAGTRIETTEAIFQAVEDKIREIIPEKDRALIVDNIGLPARAYNLAFADGST
                     IGINDGVIQVALKEGHKPTADYVRKLRQVLPAAFPEDVFYFQAADIVTQILNFGLPAQ
                     IDVRTMGYDLNNLSVARELRQRLAAIPGIVDAHLQQEVDGPAFYAQIDRTRAAQLGLN
                     ASTVATNINVSLSSSEQVSPNFWTDPTSGIPYYLAVQTPEYKVNSLNALGNTPVSTSL
                     AVSGQTVPGMLSNVATFKRDKVPTNSNQTNIQPVFDVYASVQGRDLGSVAADINKVTT
                     QLQKELKPGNSIQVIGQIQSMNDSFRNLGIGLLFAAVFVYLLMVVNYQTFGDPFVVIL
                     ALPATLCGIVTMLFITGTTLNVPSLMGAIMAVGVASANSILLVTFAREQQLKGHTAFE
                     AALSAGHTRIRPVLMTAAAMIVGMIPMAIGGAGEEQNAALARAVIGGLLFATPTTLLI
                     VPYLFAMLRKGNDGKPHHGVFEEIQA"
     gene            1950..3221
                     /locus_tag="VGA15_24190"
     CDS             1950..3221
                     /locus_tag="VGA15_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9590828.1"
                     /translation="MTEIRVRTKNEDVQERPDPATDRVVALPNPARRRRRRYGGALLG
                     GSALLLLLGGLAIGGWRHYQANLDVAATAQQSRTLVPDVRVAAVRASDSKITVTLPAT
                     TTAFEAANIFARTSGYIEKRYVDIGDRVKAGALLADITAPELDHQITQARATLTQGQA
                     TLQQTQASRELAQVTNARDSNLVKQGWLTLQQGDNDRLTLQAQQAAVGVAQSNIAAQE
                     AQIRILEQEKAYQRVVAPFDGVITQRNIDNGSLVQSGSTFMFTLMHSDVIRTQVFVPQ
                     DEAFGLGPGVDAVVRVPEIPDRTFPGKVTRIATALQPGSRTLLTEIDVPNPDGALSPG
                     IYCTVELFIPRKTPSMIIPADAVVFDQNGLHVAVVEDGTAHLQKITIARDFGNEVEVH
                     DGVKPGDQVILNPMVNLAEGSKVVARKAQTS"
     gene            complement(3218..3931)
                     /locus_tag="VGA15_24195"
     CDS             complement(3218..3931)
                     /locus_tag="VGA15_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590829.1"
                     /translation="MELQTVIDELPSGYTGDMVPVMLPTIGVGMVPIGVAGISAADDI
                     ALVDGGGTGAAAIVGGGGAGIAGGGGAGTVVPGIVDRNDVAGCADSVSIGAVVLFVDV
                     DEVAGTADIVVTAETDGIVPIVPPSADMDVTGTAAVPGAICPVGVEQVTTVPGVVGFV
                     ASGTGASVVSGAPGWVAAENGLGPLSGEVTIVPGVDESPMAVVPMVETCARLALQAAS
                     RMAVVNSKRRIAIASSLRI"
     gene            complement(4013..4294)
                     /locus_tag="VGA15_24200"
     CDS             complement(4013..4294)
                     /locus_tag="VGA15_24200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590830.1"
                     /translation="MPSGAGSGCLCRQGPGSYLLPSEKSKAGGLQQPDGDAGDTPGVA
                     AAPVALAGAIVPGVARFIVMLSAQAGVFGVGTTVIVGSMLVPASSVTAG"
     gene            4613..5290
                     /locus_tag="VGA15_24205"
     CDS             4613..5290
                     /locus_tag="VGA15_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9590831.1"
                     /translation="MAKVLLIEDDSETAEEITAELSDRGFEVEWSANGIEGLDKARSS
                     RPDAMIVDRLLPGMDGLTVIEALRKEQVRTPVLVLSALGAVDDRVRGLRMGGDDYLTK
                     PFAVVELVARIEALLRRPAESRETTLRVGPLALDLIERTAKRGDRVIDLLPREFRLLE
                     YMMQRSDQLLTRAMLLEEVWNYKFVPATNLVDVHMGRLRHKVDGPDEAPMIHNVRGAG
                     FILRAGS"
     gene            5409..6725
                     /locus_tag="VGA15_24210"
     CDS             5409..6725
                     /locus_tag="VGA15_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9590832.1"
                     /translation="MSAVFAVFVIVLFGFIYWKIDDYLTARSDRVITVELDGIAGLSP
                     ERRLEAIDEKLRQDPRGVQVAAIFAADGHRIAGNLESLPPHLRIDAPAQGTDVVRTDR
                     NSREHQVIRAIGRRMRNGDVLILGRNVDEAGEISHVVGQALALGLLPGFCLCLLAGAW
                     LSVRAQKRVEEVNQRVQRIIAGDLSERLPHRNVDEPFSKLAVIVNGMLDEMETMIHAL
                     AGVGNDIAHDLRTPLTRARLTLERGRTNAATLEQLQAVADKAIAGIDQSLTIITALLR
                     LAEIENSRRSSGFGDVALHEMLREVCDIYEPIAENKNIALQVAVRHRRSVRGDQDLLI
                     EAVANLVDNAIKFTPEGGKVNIELLQENGETIVRVTDTGCGISEQERETVLRRFYRSD
                     KIRNTPGVGLGLSLVAAIVKLHGFRLAIHSGAGGRVEIVCPDSQQA"
     gene            6978..7061
                     /locus_tag="VGA15_24215"
                     /pseudo
     CDS             6978..7061
                     /locus_tag="VGA15_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
BASE COUNT         1509 a         2416 c         2359 g         1474 t
ORIGIN      
        1 cgtctatgga ccgaagaagg catgccgctt tccgaggcga ccctgcacgg cgccgccgaa
       61 atcgcggtgc cgacgctggt ttcgacgctg gccatcagtt gcgtattcac ctcagtggtg
      121 ttcctcgacg gaccggccaa gtacctgttc acgccgctgg gcttagccgt cgtatttgcg
      181 atgctggctt cctacggatt gtcgcggacc ctcactccaa tcacgatcgg cctgctgctc
      241 aggagcgaac gtcgtcacac cgataacgac ggcgcgcccg ccggcttggc ctcacggctt
      301 tccgccgcat tcgaccgcag cttcgagcgg ttgcgcgatg gatatgcgaa attccttacc
      361 attctgcttc gccggcgagc gatcgtgcct ctcgtcgccg tgttgatgct cagtctcggt
      421 gcggtgatgc tagtgctcgt tggccgggac ttttttccgc tgatcgacgg cggccagatc
      481 cagcttcacg ttcgcgctcc cgccggtacc cgcatcgaaa ccaccgaggc gatattccag
      541 gcggtcgagg acaagattcg cgagatcatt ccggagaagg accgcgcgct gatcgtcgac
      601 aatatcggac tgccggcgcg cgcctacaat ctggcattcg ccgacggttc gacgatcggc
      661 atcaacgatg gtgtcatcca ggtcgcgctc aaggaaggcc acaagcccac cgccgactac
      721 gtcaggaaat tgcgtcaggt gctgccggcc gcattcccag aggatgtgtt ctacttccaa
      781 gccgccgata tcgtgacgca gattctgaac ttcggattgc cggcgcagat cgacgtccgg
      841 acgatgggct acgacctgaa caacctttcg gttgccaggg aactccgcca gcgcctcgcc
      901 gcaattcctg gcatcgtcga tgcgcatctg cagcaggaag tcgacgggcc ggccttttac
      961 gcccagatcg accgcacccg ggccgcgcaa cttggcctca atgccagcac ggtggccacc
     1021 aatatcaatg tcagcctcag ctcgtcggaa caggtatcgc caaacttctg gactgatccg
     1081 acgtcgggga ttccgtatta tctggccgtg cagacgccgg agtacaaagt caactcgctg
     1141 aatgcgcttg gaaacacccc ggtttccacc tcgctggcgg tgagcggcca aaccgtccct
     1201 ggcatgctca gcaatgtcgc gaccttcaaa cgggacaagg tgcccactaa ctccaaccag
     1261 accaatatcc agccggtctt tgacgtctat gccagcgtgc agggccgcga tctcggcagt
     1321 gtcgcagccg acatcaacaa ggtcacgacg cagctgcaga aggaactgaa acccggcaat
     1381 tcaattcagg tgatcggcca gatccagagc atgaacgact ctttccggaa tctgggcatc
     1441 gggctgctgt tcgccgcggt cttcgtctat ctgctgatgg tcgtgaacta tcagacgttc
     1501 ggtgatccgt tcgtcgtgat cctggctctg ccggcgacgc tgtgcggcat cgtcacgatg
     1561 ctgttcatca ccggcaccac gctgaacgtg ccgtcgctga tgggggcgat catggcggtc
     1621 ggggtcgctt cggcgaactc gattctgctc gtgaccttcg cgcgcgagca gcagcttaaa
     1681 gggcacaccg cctttgaggc cgcactcagt gccggccata cccgaatccg gcccgtgctg
     1741 atgacggcgg ccgcgatgat cgtcggcatg atcccgatgg cgatcggcgg cgccggcgag
     1801 gaacagaacg ctgcgctcgc gcgcgccgtc atcggcggcc tgctgttcgc gacgccgaca
     1861 acattgctga tcgtgcctta cctgttcgcc atgctgcgca agggcaacga cggaaagcct
     1921 caccatggcg tattcgagga aatccaggca tgaccgagat tcgtgtgcgg acgaaaaatg
     1981 aagatgttca ggagcggcct gatccggcaa ccgatcgcgt tgtcgcgctg cccaacccgg
     2041 cgcgcaggcg gcgacgtcgt tacggaggcg cgctgcttgg cggaagcgcg ctgctgttgc
     2101 tcctgggcgg ccttgctata ggaggctggc gccactacca agccaatctc gacgttgcgg
     2161 ccaccgcgca acagagccgg acccttgttc ctgacgttcg cgtcgccgcg gtccgcgcca
     2221 gcgacagcaa gatcaccgtc accttgccgg cgaccacgac ggcattcgag gccgcgaata
     2281 tcttcgcgcg caccagcggc tacatcgaaa agcgctacgt cgacatcggc gaccgggtca
     2341 aggccggagc tctgctggcg gacattaccg ccccggagct cgaccaccag attacgcagg
     2401 ccagagcgac gctgacccag ggccaggcga cattgcagca gacccaggca agccgagaac
     2461 tcgcccaggt caccaacgcg cgcgacagca atctggtcaa gcagggatgg ctcacgcttc
     2521 agcagggcga caacgatcgc ctgaccttgc aggcgcagca ggctgcggtc ggagtggctc
     2581 agtccaatat tgccgctcag gaagcccaga ttcgaatact cgagcaggag aaggcgtatc
     2641 agcgcgtcgt ggcaccgttc gacggcgtca tcacccaacg caacatcgac aacggcagcc
     2701 tggtacagtc cggatcgacc ttcatgttca ccctgatgca ttccgacgtc atccgtaccc
     2761 aggtgttcgt cccacaggac gaggccttcg ggcttgggcc cggcgtcgat gcggtggtcc
     2821 gtgtccccga aattccggat cgcacttttc ccggcaaggt cacgcgtatc gcgaccgcgt
     2881 tgcagccggg cagccggacg ctgctgaccg agatcgacgt ccccaatccg gacggcgcgc
     2941 tcagtcccgg gatctattgc actgtcgagc tgtttatccc gcgcaagaca ccatcgatga
     3001 tcataccggc cgacgcggtt gtattcgatc agaacggcct gcatgtcgcg gtcgtcgagg
     3061 acggcaccgc ccatctccaa aagatcacaa tcgcgcgcga cttcggcaac gaggtcgaag
     3121 tgcacgacgg cgtcaagccg ggcgatcagg tcatccttaa tccgatggtc aacctggcgg
     3181 aaggcagcaa ggtcgtggcg cgtaaggcgc agacaagcta gattcgtaac gaggaagcaa
     3241 tggcgatgcg gcgcttgctg ttcacgacag ccatcctgct ggcagcctgc aacgccagtc
     3301 tggcgcaggt ctccaccatg ggcaccacag ccatgggact ttcgtcgacg ccagggacga
     3361 tcgtgacttc cccactcaat ggaccgagtc cgttctcggc ggcaacccag ccgggcgctc
     3421 ccgacacgac gctggcgcca gtgccgctgg cgacgaaccc taccacgccg ggaacggtcg
     3481 tgacctgttc aacaccaacc gggcaaatcg cgcctggaac tgcggcggtg ccggttacgt
     3541 ccatgtcggc acttggcggc acaatcggga cgatgccatc ggtttccgct gtaacgacga
     3601 tatcggcagt accggctacc tcgtccacgt ccacaaatag cacgaccgcg ccaatgctga
     3661 cgctgtccgc acaaccagcg acatcattcc tgtccacaat tcccggcacg accgtaccgg
     3721 cgccgccgcc gcccgcaata ccagcgccgc ctccgcctac gatagccgca gcgcccgtac
     3781 cgccgccatc gacaagcgcg atgtcatcgg ccgcgctgat gccagcgacc ccgatgggga
     3841 ccattcccac gccgatcgtc ggcagcatga ccgggaccat atcgccagtg tacccgctcg
     3901 gcagctcatc gatcaccgtc tgcagctcca cgccgggagg tccgccgacc aatggcgcgg
     3961 ctctgccgct ctcgaccccc gaacttccga acagtcctcc gcttggcacg attcaacccg
     4021 cggtcacgga gcttgccggc accagcatcg atccgacaat gaccgtggtg ccgacgccga
     4081 atacgccggc ttgtgccgag agcatgacga tgaatctggc aacgcccggc acgatggcac
     4141 cggccaacgc cacgggtgct gcggcaacgc cgggcgtatc gcctgcatcg ccatcgggct
     4201 gctgaagtcc accggccttt gatttctccg atggcagcag gtacgaaccc gggccctggc
     4261 ggcagagaca tcctgatccc gcaccggatg gcacgcgacg gcccagagcc gcagcgaatc
     4321 ataaaaccgt atttaatggt gcggccgatt tctcgagcct atgtttgttt tcgagcggcc
     4381 gcagcgcgct cgcccccgga ggcggcgttc agatggccgt agagcggacg gcgcatgcca
     4441 cgtcttatgc ccccgagtaa gccggggcgg cgctgtccgg ctcttgatta gggtgaaatc
     4501 catcattttg caggcagctg acattatggc attggtctcg agcaagcgta cggtttcagg
     4561 ggggcgaagc tcaggcactg cgaatgcatt cgccgcaatc gagaatcagc tgatggcaaa
     4621 ggtgcttcta atcgaggatg acagcgaaac ggcggaggaa attaccgccg agctgtccga
     4681 tcgcggcttt gaagtggaat ggtcggccaa cggcatagaa ggcctcgaca aggcccgttc
     4741 gtcgcgtccc gacgccatga tcgtcgatcg gctgttgccc ggaatggatg gccttaccgt
     4801 tatcgaggca ctgcggaagg agcaggtgcg cacgccggtg ctggtgctga gtgcgctcgg
     4861 cgcggtggat gatcgcgtcc gcgggctgcg gatgggcggc gacgactatc ttacaaagcc
     4921 gttcgcggtc gtggaactcg tcgctaggat agaggcgctg ctgcgccggc ccgcggaatc
     4981 gcgcgagacg acgctgcggg tcgggccact ggcactcgat ctgatcgaac gcacggccaa
     5041 gcggggcgat cgcgtgatcg atttgctgcc gcgcgagttt cgcctgcttg aatacatgat
     5101 gcagcgcagc gatcagctgc tgacgcgagc catgctgttg gaggaggtct ggaattacaa
     5161 attcgttccc gccaccaacc ttgtcgacgt gcatatgggc cgtctgcgcc ataaggtgga
     5221 cgggcctgac gaagccccta tgatccacaa tgtccgcggc gccggcttca ttcttcgtgc
     5281 gggatcataa gcctccgatg gacggcagcg atgcgtcgct gttgacgtca gaccgtacat
     5341 ccgggcgcca agatccgatg cgtcaaccgc aattcatacg ttcgagcacc tttcactggg
     5401 cgctcgccgt gtcagccgtg ttcgcggttt tcgtcatcgt gctgttcgga ttcatctact
     5461 ggaagatcga tgactatctg acggcgcgat cggaccgggt gatcaccgtg gaactcgacg
     5521 gcattgccgg cctgtcgcct gaacgccggc tcgaggcaat cgacgagaag ctgagacagg
     5581 atcctcgagg ggtacaggtc gcggcgatat ttgccgccga cggccaccgg attgccggca
     5641 atctggaaag cctgccgccg catctgcgga tcgacgcccc tgcgcaaggc accgacgtcg
     5701 tcaggacgga tcgcaacagc cgggaacacc aggtcatccg cgcgatcggt cggcgcatgc
     5761 gaaacggtga cgtgcttatc cttggacgaa acgtcgatga ggccggcgaa atctctcacg
     5821 tcgtcggtca agcgctcgcg ctcggtctct tgccgggctt ttgtctttgt ctgctggccg
     5881 gcgcgtggct tagcgtgcgc gcccagaaac gcgtcgaaga ggtcaaccag cgagttcagc
     5941 gcatcatcgc cggtgatctg agcgagcggc ttccgcaccg taacgttgat gagccgttct
     6001 cgaaactcgc ggtcatcgtc aatggcatgc tcgatgaaat ggagacgatg atccacgctc
     6061 tcgccggggt cggtaacgac atcgcccacg acctgcgaac gccgctgact cgcgcgcggt
     6121 tgacgctgga gcgcgggcgc accaacgcgg ccacgctgga gcaattgcag gcggtagcgg
     6181 acaaggcgat cgcgggcatc gatcagtcgc tgacgatcat caccgcgcta ttgcgtctcg
     6241 cggagatcga aaacagtcgg cggtcctcag gttttggcga cgtcgcactg catgagatgc
     6301 tgcgcgaggt gtgcgatatc tacgagccga tcgccgaaaa caagaatatc gccttgcagg
     6361 tcgcagtgag gcaccgtcgc agcgtgcgcg gcgaccagga cttgctgatt gaggcggtcg
     6421 cgaacctcgt cgacaatgcc atcaagttca cgcctgaagg cggcaaggtg aacatcgagt
     6481 tgctgcagga gaatggcgag accatcgtgc gtgtgacaga taccggctgc ggcatcagcg
     6541 aacaggagcg cgagaccgta ctgcggcgtt tctaccgttc cgacaagata agaaatacgc
     6601 cgggtgtcgg tcttggcctc agcctggtgg ccgcgatcgt caagctgcac ggcttccggc
     6661 tggcgattca ttccggtgcg ggcggccggg tggagatcgt ctgtcccgat agccaacagg
     6721 cctgaagagc tatttgatgc cgcgcgcagc gtgattgggg aattcctgca tcattttctg
     6781 atttcttcaa gcgagcgagg ccgccggagc tgaagaataa aaccgcattt aatggaagcc
     6841 gacgacggcc cgcttatatt gagcgaaccg aatgcgcgac agtgaagaga cgcttattca
     6901 ttgtcggtgc cgatcatgcg ctcaatctcg gcggagcaat tccatgcgca caactcttct
     6961 cataacggtg atccactttc tgcgcccagc gttatcgctc ctacgatccc gcgtccggca
     7021 ccttccttgg ccatgacggt cgtcggcatc ggtgcgagtg aggtccaacg gtcaaagaga
     7081 cgaaggcaag cggcagtcga cggtgactgg atttcgtcac ggtccgcttt gtgaagcgat
     7141 ttgcctggtt catgggaacc tgtcctcgca gtacagggat gatgtcctct gccgccgtca
     7201 tgccgatcct gtacatggcg tccttggtcg cccgcgcatc gaaagaaggc ggcgacttcc
     7261 gattgtggtg ccatccccat cagggcatgt ccgttgatgg gcgctcaatg gtctcgcgcg
     7321 ggtgacgcga atttgcacca gcgtgttctc tgcgtggagt gggtgatcgt tggtatcgag
     7381 caggcgtgtg atgacaattt gcgcgacaca cgaattctga caaatccgac tgtagaataa
     7441 cttctttttt aatcgtcaag ttcgcccgaa ccgctatctc cttcctatct cccaggtgtt
     7501 gcatacgtcg caaactgccc tgtgcaaccg gcgaatttat tccgttgagt tcccgcggtc
     7561 caaaaacatg agcgctcagt gctcgctgga aatgaaagcc gcagcagaag ggacgtactt
     7621 ataccagatt gcgtagggac tgactctcgg agttttcggc gggttgtgat tcatttgggc
     7681 ttcacgattt gattcgggga gcaacaaatg agcgcagcgg ttgagcttcg ggatgatttt
     7741 acggcagcag atctacgt
//
LOCUS       DASWGM010000891         7069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_121607,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000891 DASWGM010000000
VERSION     DASWGM010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121607"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VGA15_24220"
     CDS             complement(<1..134)
                     /locus_tag="VGA15_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9590833.1"
                     /translation="MQIRNLGGSGLRVSAVGLGCNNFGQRTDLETSRKVIHKAIDLGV
                     T"
     gene            complement(231..1076)
                     /locus_tag="VGA15_24225"
     CDS             complement(231..1076)
                     /locus_tag="VGA15_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX9590834.1"
                     /translation="MIRIVSIVSALVVLTLVLLPFQLIGIAFNLRLQRTIPHLYHRIL
                     CALIGIRISEVGRRSTATPALILSNHVSWLDICVISALAPVVFVAKSEVAEWPVFGWL
                     AKLQRTIFINRQARHQTGAATREIAARLLGGDAVVLFAEGTSSDGIRVLPFRSSLVGA
                     VHHALGNTTRHTHITVQPMSLAYVGFGGVPMGRGLRERVAWYGDADLIPHLLQVLSSG
                     AVDVTVSWGEATAYDMSADRKAIARDAEKSVRRMTAAALRAKPPVQTPAAPVPGPRQP
                     HPRPA"
     gene            complement(1380..1841)
                     /locus_tag="VGA15_24230"
     CDS             complement(1380..1841)
                     /locus_tag="VGA15_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9590835.1"
                     /translation="MSGELSVRFVVREDYEQWLPLWDGYNAFYGRSGATALSPEITRM
                     TWARFFDAYEPVHGLVAESDGHLLGLTHYLFHRSTTAIEPVCYLQDLFTSNAARGRGI
                     GRALIDRVYEQARRTGSSRVYWQTHQTNLTAMQLYDKVAEHSGFVVYRKLL"
     gene            complement(1951..2739)
                     /locus_tag="VGA15_24235"
     CDS             complement(1951..2739)
                     /locus_tag="VGA15_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX9590836.1"
                     /translation="MQFDWRAICGPALTTVTALIAISVDAHSVAIPNPAPLFVCIVAF
                     AGSLSGLASGMISAAIALAASALFFLNHRAAPGYDATDQARLAMLAITATGTAGIAGL
                     LRRNLTNAFAWQRKRDGTAERLSAALDQVDIGIVLLDSDTRAEFINRAFRDYFSLPDE
                     KADSRPPFIALMYHGRDIGAYELPEEELSAFIARHTEMMRSGDSTPININLADGQVLR
                     FSCTALPDGGRMLSYTPVTDLVRHSDAPCSADCYQSRRRGIAAA"
     gene            2942..3295
                     /locus_tag="VGA15_24240"
     CDS             2942..3295
                     /locus_tag="VGA15_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX9590837.1"
                     /translation="MPTISPSVLPVLMLFASNVFMTFAWYAHLKFKESWLPFAIMVSW
                     GIAFFEYWLAVPANRWGSAVYSAAQLKTMQEVITLIVFAGYSVLYLKEPLGWNHGLGF
                     ALIAAGAFFIFHKWS"
     gene            complement(3477..3611)
                     /locus_tag="VGA15_24245"
     CDS             complement(3477..3611)
                     /locus_tag="VGA15_24245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590838.1"
                     /translation="MKTPAALVAAALTAAILLTASFLIATGTRGESCTLQATLRDELC
                     "
     gene            complement(3665..3847)
                     /locus_tag="VGA15_24250"
     CDS             complement(3665..3847)
                     /locus_tag="VGA15_24250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590839.1"
                     /translation="MIASSGAAGFDSRAAPVSSSRLTHAVAASIALSLCLIVTLTVFS
                     TKVTLAMPGVTQLLFP"
     gene            complement(4004..4657)
                     /locus_tag="VGA15_24255"
     CDS             complement(4004..4657)
                     /locus_tag="VGA15_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9590840.1"
                     /translation="MYKLYSMQRSGNSYKVRLALALLNAPYRAIEIDILRGESRTPDF
                     LARNPSGQVPLLEVAEGRYLAESNAILWYVAGGTSLAPESRIERAEALQWMFFEQHAL
                     EPNIGAAYFWLALVKGGRDLQTHALEDWMERGYAALQVMENHLKTHAYFATGQLTVAD
                     IALYGYTHVADHCDYDLTTFPAIRAWLRRIEQTPGFVSMDWRPCAEVDDPAGVAAEA"
     gene            complement(4878..6098)
                     /locus_tag="VGA15_24260"
     CDS             complement(4878..6098)
                     /locus_tag="VGA15_24260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX9590841.1"
                     /translation="MAHFWQGLRSGEWLTPARARGYSLILLGISALAIAGWIAVSDGL
                     IDRNGKPLGTDFSNVYAAGSLTWQGRPAEAYQPALQHAEEKAVFDGREVPFYGWHYPP
                     FFFAIAVLVAAVPYAWGLAIWLAASLAAYLAAMRAILPRPGTLLIATAFPAVFVNIGH
                     GQNGFLTAALLGGALHWLDRRPWLAGVLIGLLAYKPQFGVLIPVALLAGGRWSSIAAA
                     AATVATLLAVSFVTLGGDVWHAFADSMTFTQTVVLEQGSTGWEKIQSVFSAVRMWGAG
                     VQLAYAVQIALALMLATSLGWLWQSDAAFELKASALATASLLATPYVLDYDLVALAVG
                     IVFFVRHGLSRGFRTFEISLLAAAWIAPLLSRGVAGAAGIPLGLMVLLALYVFTLRRA
                     LIDRATIVNEIAQA"
     gene            6311..>7069
                     /locus_tag="VGA15_24265"
     CDS             6311..>7069
                     /locus_tag="VGA15_24265"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX9590842.1"
                     /translation="MPFLSAALDRVKPSATIAVTDKARELKAAGRNVIGLGAGEPDFD
                     TPANIKLAAIHAIEAGKTKYTAVDGIPELKQAIIAKFARENGLTFKPNQIIVGTGGKQ
                     VLYNALMATINPGDEVIIPAPYWVSYPEMVALAGGEPVPVVCTAEQGFKLQPDALEKA
                     ITPKTKWIILCSPSNPTGAAYTRAELKAITDVLVKHPDVWVMTDDMYEHLVYDDFVFT
                     TPAQVEPKLYNRTLTVNGVSKAYCMTGWRIGYAGG"
BASE COUNT         1414 a         2117 c         2278 g         1260 t
ORIGIN      
        1 gtgacgccga ggtcgatcgc cttgtgaatc accttgcgcg aagtttcgag atcggtgcgc
       61 tggccgaaat tgttgcagcc gaggccgacg gcggaaacgc gcaggccgga gccgccgaga
      121 ttgcgaattt gcatggactg atcctgtagg gtgtgggcgc gaggtgggat cgtattgtga
      181 cgcgtgcgcc gcaaggctgc aaggtacggg gtgccatcgc acggtgcgcg tcaggccggt
      241 cggggatgcg gttgccgcgg tccggggacc ggcgcggccg gagtttgcac aggcggtttg
      301 gcgcggagcg ccgccgcggt catgcgccgc accgattttt cggcgtcgcg cgcaattgcc
      361 ttgcgatcgg cgctcatgtc gtaggccgtg gcttcacccc agctcacggt gacgtcgaca
      421 gctcccgacg acagcacctg aagcagatga gggatcagat cggcgtcgcc gtaccatgcc
      481 acccgctcgc gcaagccccg gcccatcggc acgccgccga acccgacata ggccagcgac
      541 atcggctgca ccgtgatgtg ggtgtgacgc gtggtgttgc cgagcgcgtg atggaccgcg
      601 ccgaccagcg aggagcgaaa cggcagcacg cggatgccat cgctcgacgt tccctcggca
      661 aacagcacca ccgcgtcgcc gccgagcagc cgggctgcga tttcccgggt cgcggcgccg
      721 gtctgatgcc gcgcctgccg gttgatgaag atcgttcgct gcagtttcgc gagccagccg
      781 aacaccggcc attccgcgac ctcgcttttg gcgacgaaaa ccaccggcgc cagcgcggag
      841 atgacgcaga tgtccagcca ggacacatga ttggacagga tgagtgcggg ggtagccgtc
      901 gatcgccggc cgacctcgct aattcttatc ccgatcagcg cgcagagaat acgatgataa
      961 agatggggaa tggttcgctg cagccgcagg ttgaaagcga tgccgatcag ctggaacggc
     1021 agcagcacga gcgtaagtac gacaagggcc gacacgattg atacgatgcg gatcatgaaa
     1081 gcggttcgtt gaacgtggct gctcgcgcag cgagacagcg gaggccgtcg cataaccgtt
     1141 atacgtgatg caagcagtgt ggtgacaaaa aagtgcggcc ccggtcagac gcggcgactg
     1201 acggggaccg ctttggggcg tgtgatctgt gacggggggc ctgatcagac gccaatgcaa
     1261 cctagctgtg ctttgttacg tgccggtgac agccggactt atccacaggc cggttcagcc
     1321 tcggtccgcg ggctcatctg aacccgccgg gcgggggtgg gcgcgcaaag tgcagctcgt
     1381 cagagcagct tgcgatacac gacgaatccg gagtgctcag cgaccttgtc gtaaagctgc
     1441 atcgcggtga ggtttgtctg gtgcgtttgc cagtacaccc gcgatgatcc ggtacgccgc
     1501 gcctgctcat agacccgatc gatcaatgct ctgccgatgc ctcttccacg cgcggcattg
     1561 ctggtgaaca aatcctgcaa atagcagaca ggctcgatgg ccgtggtgct gcggtggaac
     1621 agatagtgcg tcaggccgag taagtgtccg tcgctctccg cgaccagacc atgcaccggc
     1681 tcataggcat cgaagaagcg cgcccacgtc atacgcgtaa tttcgggcga aagcgcggtc
     1741 gcgcccgacc gcccgtaaaa cgcgttgtat ccgtcccaca aagggagcca ttgctcgtag
     1801 tcctcgcgga ccacaaagcg gacagacagc tcgccggaca tttttgcttc tgcatttcat
     1861 gagggagcaa tcagggtggg cgcgggcggg tgatctcgtc cccttattag agacaccgcc
     1921 gagaaggatc aatctggttc gcgttcgaag ctatgccgcc gctattccgc ggcgccgcga
     1981 ctggtagcaa tcggcgctgc agggggcgtc gctgtggcgg acgaggtccg tcaccggcgt
     2041 gtaactcagc attcgtccgc cgtccggcag cgccgtgcaa ctgaagcgca gcacctgtcc
     2101 gtcggcgaga ttgatattga tcggggtcga atcccccgat cgcatcatct cggtatgccg
     2161 cgcgatgaag gcgctcaact cttcctccgg cagttcatag gcgccgatgt cgcggccgtg
     2221 atacatcagc gcgatgaatg gcggcctgct gtcggccttc tcgtccggca gcgaaaaata
     2281 atcgcggaag gcgcggttga tgaattccgc gcgggtgtcg gaatcaagca gcacgatgcc
     2341 gatgtcgacc tggtccagcg ccgccgacaa acgctccgcg gtcccatcac gcttgcgctg
     2401 ccacgcgaac gcgtttgtca agtttctccg cagcaggccg gcgatgccgg cggtgccggt
     2461 cgcagttatc gccagcatcg caagccgagc ctgatccgtt gcgtcataac caggcgccgc
     2521 gcggtggttg agaaaaaaca gcgccgaagc cgcgagcgcg atggctgcgc tgatcatgcc
     2581 cgaggcaagg ccgctcagcg agcctgcgaa agcgacgatg cagacaaaca gcggtgcggg
     2641 gttgggaata gcgacagaat gagcgtcgac agagatggcg atcaaagccg tcactgtcgt
     2701 cagagccgga ccgcagattg cgcgccagtc gaactgcatg cctgttctcg ttgcctgcga
     2761 gaagaagtga cccagcttga ccgatggtgg cgatcgcggc ggggccttcc ggctcgatgc
     2821 ttaagaggcg gttgaccgaa ccggtgcggc tttccgattt gttggatcaa atgctatcga
     2881 ttgcgggcgg aaccgggttc cacgctgatg ggttagcgct gaaaatttcg aaaggatgtc
     2941 catgcctacg atttcgccgt cggttttgcc ggttctgatg ctgttcgcct ccaatgtctt
     3001 catgacgttc gcgtggtacg cccatttgaa gttcaaggag agctggctgc cgttcgccat
     3061 catggtgagc tggggcatcg cgttcttcga atattggctg gcggtgccgg ccaatcgctg
     3121 gggcagcgcg gtctattcgg cggcgcagct caagacaatg caggaggtga tcacgctgat
     3181 cgtgttcgcc gggtactccg tcctgtacct gaaggagccg ttgggctgga atcacggatt
     3241 ggggtttgcg ctgatcgccg ccggcgcgtt ttttattttt cacaagtggt cgtgaggttg
     3301 gtaagcgtcg cgccacactt tgtcgtcata cccgcggaaa gcgggtatcc agtatgctgc
     3361 agtcttgcca ttcaatcacc agccaccagc gccctggaat ctggatcatc cgctttcgcg
     3421 ggtgacgacg ttgccgggtg cgggcggatc cgccgccctc ttcgcagccg gcggcgtcag
     3481 cacaattcgt cccgcagggt tgcctgtagc gtgcagcttt cgccgcgggt tcccgtggcg
     3541 atcaggaatg acgctgtcag caagatcgcc gcggtcagcg ccgcggcaac aagggcggca
     3601 ggtgttttca tcgtgacccg atggcgatgc cgatcaccgc gttggttcgc aaaattgcga
     3661 acggtcaggg aaataataat tgagttacgc cgggcatcgc caacgtgacc ttggtcgaaa
     3721 acaccgtgag ggtgacgatc agacacagcg acaacgcgat cgaggccgcg acggcatggg
     3781 tcagtctgga cgatgagaca ggggccgcgc ggctatcaaa gccggcagca ccggacgacg
     3841 ctatcatgag ccgaaatcct tgaatgcgca ggcgaatcac ctgcggcacc caagaatctt
     3901 gcagcaatcg cgggcaacat tggggcagaa ctgccttgaa aatggcaaaa tggcgacgcg
     3961 gttagcgtcg tggtggagcg gccatagccg ttatttttat agcttatgcc tcggccgcga
     4021 cgccggccgg atcgtcgact tcggcgcagg gtcgccagtc catcgaaacg aatcccgggg
     4081 tctgctcgat ccgccgcaac caggcgcgga tcgcggggaa ggtcgtgagg tcgtagtcgc
     4141 agtggtcggc gacatgggta tagccgtaga gcgcgatatc cgcgaccgtg agctggccgg
     4201 tggcaaaata ggcgtgggtc ttgaggtgat tttccatcac ctgcaacgcc gcatagccgc
     4261 gctccatcca gtcttccagc gcgtgggtct gcaggtcgcg gccacccttg acgagcgcca
     4321 gccagaaata ggccgcgccg atgttcggct ccagcgcgtg ctgctcgaag aacatccact
     4381 gcagcgcctc ggcgcgttcg atccgcgatt ccggcgccag cgaggtgccg ccggcgacat
     4441 accagaggat ggcgttggac tcggcgaggt agcgtccctc cgcgacttca agcagcggca
     4501 cctggccgct gggattcctc gcgagaaaat ccggcgtacg gctttcgccg cgcagaatgt
     4561 cgatctcgat cgcgcgatag ggcgcgttga gcagtgccag cgcaaggcgg accttgtagc
     4621 tgttgccgga gcgctgcatc gaatagagct tgtacattcc ggagttcgtc cgatcggtgg
     4681 cgcgctgtat ctggtgagtg agaacgtctt gtcgctgcgc aacgcggcta aacaatgatg
     4741 ccgataacga tgcgtcgcaa ggcccaagat ctttagaggc cataaaaagt caaagacctt
     4801 gcgaagttaa cgaccggaaa aagttgaaaa cctctaccgc actgcaaccg cgcagtagat
     4861 tttactaaca aatccgttca cgcttgcgcg atttcattaa caatggttgc gcgatcgatc
     4921 aaggcgcgcc gcaatgtgaa aacgtagagg gccagcaaca ccattaggcc aagcggtatg
     4981 ccggcggcgc cggcgacgcc gcgcgacagc agcggcgcga tccaggctgc ggccaagagg
     5041 ctgatctcga aagtgcggaa gccgcggctc agtccgtggc gcacgaaaaa tacgattcca
     5101 accgccagcg cgacgagatc atagtcgagc acatagggcg ttgcgagcaa acttgccgtc
     5161 gccaaagccg aggccttgag ttcgaatgcg gcgtcgctct gccacagcca tccgagactt
     5221 gtggcaagca tcagcgccag cgcgatctgt acggcgtagg ccaactgaac gcctgcgccc
     5281 cacatccgca ccgcggaaaa taccgattga attttctccc agccggtact gccttgctcg
     5341 agcaccaccg tctgcgtgaa ggtcatggag tccgcgaagg cgtgccagac atcgccgccg
     5401 agtgtgacga agctgaccgc gagcagcgtc gcaacggttg ctgcggccgc agcgatgctg
     5461 ctccatcgtc cgccggccaa aagtgccacg ggaatcaaaa cgccgaattg cggcttgtag
     5521 gcgaggagac cgatcagcac gcctgcgagc cagggccggc gatccagcca atgcagcgcg
     5581 ccaccgagca gcgccgcggt cagaaatcca ttctggccat ggccgatgtt gacgaacact
     5641 gcggggaacg ccgtcgcgat cagcagcgtt cccggacggg gcaggatggc gcgcatcgcc
     5701 gccagatagg ccgcgagact tgccgccagc cagatcgcaa gaccccacgc atagggaacg
     5761 gcggcgacca gaacggcgat cgcaaagaag aacggcggat aatgccagcc gtagaacggc
     5821 acctcgcgac cgtcaaatac ggctttctcc tcggcatgct gcagcgccgg ctgataggct
     5881 tccgccggcc ggccctgcca ggtcagcgag cccgcggcgt agacattgga gaaatcggtg
     5941 ccgagcggct tgccgttgcg atcgatcagg ccgtccgaaa ccgcgatcca gccggcgatc
     6001 gcgagggcgc tgatgccgag caggatcagg ctgtagcccc gcgcacgcgc aggtgtcagc
     6061 cattcgccgg atcgcaaacc ttgccagaaa tgagccattt cgccgcgaaa tgttatgaat
     6121 gttgcgccac gctaaccggc cccttttaac gttccctaaa gtttggctgg accgaccaac
     6181 cttcttgcga tgcggcgtga cgcgccttag ggtgcggcga ttccaacaag aagagtcgtg
     6241 gatcggtgcg gcggttcgga agttagtccc ccgctaccgg ccagttcacg acggttgcaa
     6301 ggagatgatg atgccgtttc tgtccgcagc gctcgaccgt gtgaagccgt ccgcgaccat
     6361 cgcggtcacg gataaagcac gagagctcaa agcggcaggc cgcaacgtca tcggcctagg
     6421 cgccggcgag cccgacttcg acacccccgc caacatcaag ctggcggcga ttcacgccat
     6481 cgaggccggc aagaccaaat acaccgcggt ggacggcatt cccgagctga agcaggcgat
     6541 catcgccaag tttgcccgcg agaacggtct caccttcaag ccgaaccaga tcattgtcgg
     6601 gaccggcggc aagcaggtgc tttacaacgc gctgatggct accatcaatc cgggtgacga
     6661 ggtcatcatt cccgcgccct attgggtaag ttatccggaa atggtggcgc tcgccggcgg
     6721 cgaaccggtg ccggtggtgt gtacggcgga gcagggtttc aagctgcagc ccgacgcgct
     6781 cgagaaggcg atcacgccga agaccaaatg gatcatcctg tgctcgccgt cgaacccgac
     6841 cggcgccgcc tatacgcgcg ccgagttgaa ggcaatcacc gacgtgctgg tgaagcatcc
     6901 cgacgtctgg gtcatgaccg acgacatgta cgagcatctg gtctacgacg atttcgtctt
     6961 cacgacgccg gcgcaggtcg aaccgaaact gtacaaccgc acgctgacgg tgaacggcgt
     7021 gtcgaaagcc tattgcatga ccggctggcg tatcggttac gccggcgga
//
LOCUS       DASWGM010000892         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1330746,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000892 DASWGM010000000
VERSION     DASWGM010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330746"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VGA15_24270"
     CDS             <1..726
                     /locus_tag="VGA15_24270"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9590843.1"
                     /translation="ANNFLELLAVTDAAAIPAAAPGRFSFAAHNQDFLAAAEGMSMLA
                     WHSADAHADAARFKANGIGAYAPFDFGRDAVLTGGATARVEFSLAFAADPAMPGIAFF
                     TCQQRHPPELFWKPDYQRHPNGALRVIEVVMSAPEPAAHQDFLERITESATELAPGRL
                     TVGKTGDRITVLSPLEIARRLPGLAADGPPRFCAARLAVADLDATKRGLQRNGVDFQV
                     TDGVLLIAPAAGLALEFVEQGTI"
     gene            728..877
                     /locus_tag="VGA15_24275"
     CDS             728..877
                     /locus_tag="VGA15_24275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590844.1"
                     /translation="MAQVTRHNPAAIHAPSGGYSMGLELGQHRRLLFVSGRLNSSLSL
                     LAITN"
     gene            complement(852..1769)
                     /locus_tag="VGA15_24280"
     CDS             complement(852..1769)
                     /locus_tag="VGA15_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX9590845.1"
                     /translation="MYLDTPPRPIGTEVFSAMPDHYRRKGVRTDWADANRPGQPTDCF
                     IEGPSFDADGNLYVVDIPFGRIFRIAPDGQWSLVTEYDGWPNGLKISADGRILVADYM
                     HGIMELDAQTGRMTQVLTARHSESFRGCNDLHIASNGDIYFTDQGQTGLHDPTGRVYR
                     LARSGRLDCLIDTGVSPNGLVLDPTESVLFVAMTRDNAVWRAPFMKNGNVSKVGRFCS
                     TFGPSGPDGLTIDEAGRLFVAHASLGHVFVFAPNGELIARIKSCAGPSCTNVAIGGKN
                     RDRLYVTESVTGTVLVADISVLNSLSPAG"
     gene            2065..2940
                     /locus_tag="VGA15_24285"
     CDS             2065..2940
                     /locus_tag="VGA15_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX9590846.1"
                     /translation="MGGAAIAPLRRGLAVLLSALSDHAQAAFVRHFDGGYDPSPLDGP
                     RQRGDGGIAVRNLSVGYGEHLALEGVTGDFAPASMTAVVGPNGAGKSTLLKALAGIIR
                     PMAGEVACAALARHRLAYLPQQAELDRGFPITLGELVALGDWRNFGSVRKPSSSLAAD
                     VHEAVATVGLEALINRQIAELSVGQFQRALFARLLLQDADVILLDEPFAALDERTTDE
                     LLNLLQRWREKGQTIVAVLHDLDRVRLHFPSTLLLARSPIAWGDSSLSLSADNLARAH
                     VALGLPDGARFGRAA"
     gene            2937..>3212
                     /locus_tag="VGA15_24290"
     CDS             2937..>3212
                     /locus_tag="VGA15_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX9590847.1"
                     /translation="MSGQLLGLLIGPFEAFGFMRIALVASFALALANGPIGTLLLMRR
                     ESLSGNVLSHAVMPGAALGYLYAGYSLAALSFGGLLTGLVVAGLAALK"
BASE COUNT          539 a         1023 c         1022 g          628 t
ORIGIN      
        1 gcgaacaatt ttctcgagct gctggccgtc accgacgccg cggcaatacc ggccgccgcg
       61 cccggccgat tcagcttcgc tgctcacaac caggattttc tcgccgcggc ggaggggatg
      121 tcgatgctgg cctggcacag cgccgacgcg catgccgatg cggcgcgctt caaggcaaat
      181 ggcatcggcg cctatgcgcc gtttgatttc ggccgtgatg cggtgctcac gggcggagcg
      241 acggctcggg ttgaattttc gctcgccttc gcggctgacc ccgccatgcc cgggatcgct
      301 ttcttcacct gccaacagcg tcatccaccg gaactgttct ggaagccgga ctaccagcgc
      361 caccccaacg gcgccttgcg cgtgatcgag gtcgtgatgt cggcgccgga accggcggcg
      421 caccaagact tccttgagcg catcaccgaa agcgcaaccg agcttgcgcc cgggcggctg
      481 acggtcggca agacaggcga ccggatcacc gtgctcagtc cgcttgagat cgcccgtcgg
      541 ctgccgggtt tggctgccga cggtccaccg cgtttttgcg cggcgcggtt ggccgtcgcc
      601 gatctggatg cgaccaagcg ggggttgcag cgcaacggtg tcgacttcca ggtcactgac
      661 ggcgttctgc tgatcgcgcc ggcagcgggc ctggctctgg agttcgtcga acaggggaca
      721 atctgacatg gctcaagtta cccgccataa tcccgcagcc atccacgctc cgtccggcgg
      781 ctacagcatg gggctcgaac ttggccaaca tcgccggctg ctgtttgtca gcggccggct
      841 taattcgtcg ctcagcctgc tggcgataac gaattgagaa cgctgatatc cgcgaccagt
      901 acggtgcccg tcactgattc cgtcacatag aggcggtcgc ggttcttgcc gccgatcgcg
      961 acattggtgc agcttggtcc agcgcaggat ttgatgcgcg cgatcaactc gccattgggc
     1021 gcgaacacga acacatgccc gagcgaggcg tgcgccacga acagccggcc tgcctcgtct
     1081 atcgtcaggc catcgggacc gctcggaccg aaggtcgaac agaagcggcc gacttttgaa
     1141 acattgccgt tcttcatgaa aggcgcgcgc cagaccgcgt tgtcgcgggt catcgcgaca
     1201 aacagcacgc tctcggtggg gtcgagcacc aggccgttgg ggctgacgcc ggtgtcgatc
     1261 aggcagtcga gccgtccgct ccgcgcgagc cgatagaccc gccccgtggg atcgtgaagc
     1321 ccggtctgtc cctggtcggt gaaataaatg tcgccgttcg atgcgatatg cagatcgttg
     1381 cagccccgaa aagattcgga atgacgcgcc gtcaaaacct gcgtcatgcg tcctgtttgt
     1441 gcatcgagct ccatgatgcc gtgcatgtag tcggcgacca gaatgcgtcc gtcggcgctg
     1501 atcttgagac cgttgggcca gccgtcatat tccgtgacca gcgaccattg accgtccggc
     1561 gcaatccgga agatgcgtcc gaacgggata tccacgacgt agagattgcc atcggcatcg
     1621 aacgacgggc cctcaataaa gcagtcggtc ggctgacccg gccggttggc gtcggcccag
     1681 tccgtccgca cacccttgcg ccggtaatga tcgggcatcg ccgaaaatac ttcagtgccg
     1741 atcgggcgcg gcggagtgtc gagatacatc atcgcgtggt ttcacctgtg agagacgaag
     1801 ggcatcctaa aggagcatgt ccacgccgtc gacgtcttgg gggcgcgcaa ggcgcggctg
     1861 tgtaaagacg tggctcatcg atacttcagg ttttcggtac acacagtcag cggagtgcca
     1921 aggcgccaaa tgccccagga ttgaccctcc tgaatgcctc ccctagacta cgcgcgcggt
     1981 tacgacattc cttcacccgg acttgtaatg gccaattctt cggatggcgc gcgctggcgt
     2041 cggtctttcc aagagatggt cagcttgggg ggcgccgcta tcgcaccgct gcggcgtggg
     2101 ctggctgttt tgctttcggc gctgagcgat cacgcgcaag ccgccttcgt ccgccatttc
     2161 gatggcggct acgatcccag cccgctcgac gggcctcgtc agcgcggcga cgggggaatc
     2221 gcggtccgca atctttccgt gggctatggc gaacacctcg cgctggaagg cgtgaccgga
     2281 gattttgcgc cagcgagcat gaccgccgtc gtcggcccaa acggagccgg gaagagcacg
     2341 ctcttgaaag cgctcgccgg gattatcagg ccaatggccg gcgaggtcgc ctgtgccgcg
     2401 cttgcgcgcc atcgcctggc ctatctgcca cagcaggccg aactcgaccg cggctttccg
     2461 atcacgctcg gcgaactcgt cgcactcggc gattggcgaa atttcggctc agtccgcaag
     2521 ccgtcatcaa gccttgccgc tgatgtccat gaggcggtcg cgaccgtcgg ccttgaagcc
     2581 ttgatcaatc ggcaaatagc cgaactctcc gtcggccaat tccagcgggc tctatttgcc
     2641 cggctgctgt tgcaggatgc ggacgtcatt ttgctggatg aaccattcgc cgccctggat
     2701 gagcgcacga ccgacgaact gctgaatctg ctgcagcggt ggcgagagaa agggcagacg
     2761 atcgtcgcgg tgctgcatga cctggaccgg gtgcgtctgc atttcccctc gacgctgctg
     2821 ttggcgcgct cgcccatcgc atggggtgac agctcgctct cgctttcggc cgacaatctt
     2881 gcgcgcgccc acgtggcgct gggtctgccg gatggcgctc gctttggacg tgccgcgtga
     2941 gtggacagct tcttggcctg ctgatcggac cgttcgaggc gtttggcttt atgcgaatcg
     3001 cgctggtcgc gagcttcgct ctggctctgg caaacgggcc catcggcacg ctgctactca
     3061 tgcgtcgcga aagcctgagc gggaatgtac tgtcgcatgc ggtgatgccg ggcgcagcac
     3121 tcggctatct ctacgccggc tattccctgg ccgccctgag tttcggtggt ctgctgactg
     3181 gccttgtcgt cgccggcctg gcggcgctca ag
//
LOCUS       DASWGM010000893         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1200922,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000893 DASWGM010000000
VERSION     DASWGM010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1200922"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="VGA15_24295"
     CDS             complement(<1..1705)
                     /locus_tag="VGA15_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9590848.1"
                     /translation="MNSHGYPTLRRILLAAVLLLVCQPAFAEKRVALVLGNSAYQNVP
                     QLTNPVNDGAVVAATLKAAGFDVVDSRHDLPAAETRRALRDFSDRARDADIAVVYYAG
                     HGMEVDGTNYLIPVDAKLERDTDVYDEALSLDRVLVAIEPATRLRLVILDACRDNPFA
                     KSMKRTLASRAIGQGLGKVEPSSPNMLIAYSAKAGSTALDGDARNSPFTLALAKHLTT
                     PGLDVRRAFGFVRDEVLKTTGNKQEPFVYGSLGGDDVPLVPAAKVAPSMPAPNPQAEA
                     RRDYELALQIGNKDALNAFLAQYPDGFYANLAKIQVAKIAAEETRVAATEKARLAEQE
                     RARLAAEGTQKTQQLKAAEADAKAAEQARIAAEKAKQVAQEQAAAAEQKRAAAEIADK
                     LSEKIASINTAADRGSASPEPAEKAQGDRQGDKQGEKAVNVASLTAGPPPADLTKSVQ
                     IELRRVGCLTADADGEWNSASQRSMALFNRHAKTRFDVKVASVDALDAIKLKPARVCP
                     MICQHGFEADGNRCSKIVCGEGSFLNDDNECEKRRAKTPTARRDREDRNYRDDRADRP
                     VR"
     gene            1953..>2945
                     /locus_tag="VGA15_24300"
     CDS             1953..>2945
                     /locus_tag="VGA15_24300"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX9590849.1"
                     /translation="MRGVVFTGERGLELMPFPDPTPDAHDVVIEMKASGMCGSDLHQY
                     RRPRGQTQATGIPMRDGPVIAGHEPCGVVVAVGAAVDAKEARVGQRAMVHHYQGCTQC
                     SHCRSGWQQLCQEVPVKVYGNNAHGGHAQYLKVPANTLVPLPDELSFSAGAAIACGSG
                     TAYSALRRMNLSGNDTIAIFGQGPVGLAATQYAKAMGAKVIALDISSQRLERAKAFGA
                     DAVVNPGSNDPVAAIRDLTHGRYADLTLDTSSNPEARLNAIRSTKVWGTMCFVGEGGD
                     VHIDVSPHLLRRQLTLVASWTFSNIIQADCARFSVERGIDVDKLFTHRWAIDQAD"
BASE COUNT          503 a          964 c          915 g          563 t
ORIGIN      
        1 cccgaaccgg gcgatcggcg cgatcgtcac gatagttgcg atcctcacgg tcgcgccggg
       61 ccgtcggtgt tttcgcgcgc cgcttttcgc attcattgtc gtcattgagg aacgagccct
      121 ccccgcagac gattttgcta cagcggttgc catcggcctc gaagccgtgc tggcagatca
      181 tcggacagac gcgcgccggc ttgagcttga tcgcgtcaag cgcatcgaca ctcgcaacct
      241 tgacatcgaa tctcgtcttg gcgtgacggt tgaacagcgc catcgagcgc tgcgaggccg
      301 agttccattc accatcggca tcggcggtga gacagccgac gcgacgcaac tcgatctgaa
      361 ccgatttggt cagatcggcc ggcggcggcc cggcggtcag cgaggcaacg tttacggctt
      421 tctcgccttg tttatcgccc tgtctatcgc cctgggcctt ctcggccggc tccgggctgg
      481 ctgaccccct gtccgcggcc gtattgatgg aagcgatctt ctcggaaagc ttgtcggcta
      541 tttcggctgc ggcacgtttt tgctcggccg cggcggcctg ttcctgcgcg acctgcttgg
      601 ccttttcggc cgcaatacgg gcctgctccg ccgctttcgc atccgcttcg gcggccttga
      661 gctgctgggt cttttgtgtg ccctcggcgg caagccgcgc ccgctcctgt tcggccaacc
      721 gcgccttttc ggtcgccgcg acgcgggttt cctcggcggc aatcttggcg acctggattt
      781 tggccaggtt tgcgtagaac ccatcaggat attgcgcgag gaaggcgttc aatgcgtcct
      841 tgttgccgat ctgcagcgcc agttcgtaat cccggcgcgc ctccgcctgg ggattgggcg
      901 cgggcatgga tggggcgacc ttggctgccg gcaccaacgg cacatcgtcg ccgccgagcg
      961 agccatagac gaagggctcc tgcttgttgc cggtggtctt cagcacctcg tcgcgcacga
     1021 aaccgaacgc ccggcgcaca tcgagccccg gcgtcgtcag gtgctttgcc agtgccagcg
     1081 tgaacgggct gttcctggca tcaccgtcca gcgccgtcga gccggccttg gcggaatagg
     1141 cgatcagcat gttcggactg ctcggctcga ccttgccaag accctgtccg atcgcgcgtg
     1201 atgccaaggt ccgcttcatg cttttggcga acggattatc gcggcaggca tcgagaatca
     1261 ccagccgcaa gcgcgtcgcg ggctcgatcg ccaccagcac acgatccagc gaaagcgctt
     1321 cgtcgtagac atcggtgtcg cgttccagct tcgcatcgac cggaatcagg taattggtgc
     1381 cgtccacctc catgccgtgg ccggcgtaat agaccaccgc gatgtcggcg tcgcgcgcgc
     1441 gatcggaaaa gtcgcgcagc gcgcgccgcg tctcagccgc cgggaggtcg tggcgtgaat
     1501 cgacgacgtc gaagccggca gccttgagcg ttgctgcgac caccgcgccg tcattgaccg
     1561 ggttcgtaag ctgcggcacg ttttgatagg ctgaattgcc gagcacaagg gccacgcgtt
     1621 tttcggcgaa agccggctgg cacaccagca gcagcacggc cgccagcaaa attctgcgca
     1681 atgttggata gccatgcgaa ttcatgatgt cacatccgaa acttcggccc tttatagcac
     1741 tgccgcctga ctaccagaac cctgcgccgg accatgtgag ccggatcacg catttgacgc
     1801 agatgccgtc gtatgtatcg attgccgcgg tgtcagagcc gctgacggaa ctccccctca
     1861 ttagtcgcgc cagatcgctg tcggttcgcc ctttgccgat cacatttttc cgtccaagat
     1921 cgggccacaa acaataacaa acggaggaaa ccatgcgcgg cgttgtgttc acgggcgaac
     1981 gcggactgga actgatgccc ttccccgatc ccacaccgga tgcccatgac gtggtcatcg
     2041 agatgaaggc ctccggcatg tgcggcagcg acctgcatca ataccgccgc ccgaggggcc
     2101 agacccaggc caccggcatt ccgatgcggg acggtcccgt catcgccggg cacgaaccct
     2161 gcggcgtggt ggtcgcggtc ggcgccgcgg tcgatgccaa ggaggcgcgc gtcggccagc
     2221 gggcgatggt gcaccattat caaggttgca cccagtgcag tcattgccgc tccggatggc
     2281 agcagctctg ccaggaggtg ccggtcaagg tctacggcaa caacgcccac ggcggccacg
     2341 cgcaatattt gaaagtgccc gccaacacac tggtgccgct gcccgacgag ctatcgttct
     2401 cggcgggggc tgcgatcgcc tgcggttccg gcaccgccta cagcgcgctg cgccgcatga
     2461 acctttcagg caacgacacc atcgcgatct tcggccaggg gccggtcggg ctggcggcca
     2521 cgcaatatgc caaggcgatg ggcgcgaaag tgatcgcgct cgacatcagc tcgcaacggc
     2581 tcgagcgcgc aaaagcgttt ggcgccgatg cggtggtcaa tcccggctcg aacgatccgg
     2641 tggcggccat cagggacctc acccacggac gttacgccga tctgacactc gacacctcga
     2701 gcaatccgga ggcccggctg aacgcgatcc gctctaccaa ggtctggggc acgatgtgct
     2761 tcgtcggcga aggcggcgac gtccatatcg atgtcagccc gcatctgctg cgccggcaac
     2821 tgacgctggt ggcgtcatgg acattttcaa atatcattca ggcggattgc gctcggtttt
     2881 cggtcgagcg cggcatcgac gtcgacaaac tgttcaccca tcgctgggcc atcgatcagg
     2941 cggac
//
LOCUS       DASWGM010000894         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2192222,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000894 DASWGM010000000
VERSION     DASWGM010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192222"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VGA15_24305"
     CDS             complement(<1..1031)
                     /locus_tag="VGA15_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX9590850.1"
                     /translation="MSEQIRSWLSSQRDAMLSLIEKVVNIDSGSYDKAGVDAVGEVFE
                     EFFKSHGIEVERLPRDVSGDIFRAGIPGPGNAPIVLMGHRDTVFSKGEAARRPFTIRD
                     GRAYGPGVTDMKGGLVLNAFVLAAFKKFGGNAAPLVALLTSDEEIGSPQSRGVIEDTA
                     RGARAVYNAEPGRPNGNVVSQRKGGVFMVMDVVGKAAHSGGNFERGHSAINALAHKTL
                     AIAALTDLSIGVTLNIGLVSGGQSVNTVAPSATGQIDMRYARPADRDRIFAAIEEIIA
                     TEHVPGTSATLRIKGEFLPLEHTAGNAGLLEIYQKAAKQAGLALEGEATGGCADSGFT
                     SAVGTPTLCA"
     gene            complement(1086..1364)
                     /locus_tag="VGA15_24310"
     CDS             complement(1086..1364)
                     /locus_tag="VGA15_24310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590851.1"
                     /translation="MKPRDVGLVEKPDITRPIEPRAPSIRCTERELFSQPRVTGILVE
                     TGVPGGVLMPVLASGTIPCGEWVRFVMLPIAKWFVICIGALIIGVWID"
     gene            complement(1361..1678)
                     /locus_tag="VGA15_24315"
     CDS             complement(1361..1678)
                     /locus_tag="VGA15_24315"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX9590852.1"
                     /translation="MKFANFITYMPDPQKVQSVRPLHRDYAQGLASQGKIVIAGPFSD
                     GAGAIIVYEAQSLEQAEALAANDPYAKGGVWTRYEIHPWDIIGVNHALLPNMANGKSS
                     QPH"
     gene            complement(1701..>2429)
                     /locus_tag="VGA15_24320"
     CDS             complement(1701..>2429)
                     /locus_tag="VGA15_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029688.1"
                     /note="FMN-linked; catalyzes the formation of
                     N-ethylsuccinimide from N-ethylmaleimide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX9590853.1"
                     /translation="RLPYETPRALEIDEIQRIVDEYDQAAARAMLAGFDGVELHGANG
                     YLIDQFLQDRTNHRSDRYGGSVENRVRFLLEVTDRLVSRLGASRVGVRLSPFSTVNDI
                     SDSDPSALFRHAIGALSCRRIAYLHLIEPRVNAGLREEPNLNVPSSVAAMFRESFEGP
                     LIASGGFTKASADAAIKAGIVDAIAFGRAFIANPDLPHRLATGAPLNAYDRPTFYGGA
                     EQGYTDYPFLQPQRGAAQEFVSPA"
BASE COUNT          481 a          752 c          708 g          488 t
ORIGIN      
        1 gcacacagtg tcggtgtgcc gacggccgac gtaaagccgg aatcggcgca accgccggtg
       61 gcttcgcctt ccaaagcgag gcctgcctgc tttgccgcct tttgataaat ctccagcagg
      121 ccggcattgc cggccgtgtg ctcgagcggc aggaactctc ccttgattct cagggtcgcc
      181 gaagtgccgg gcacgtgttc tgtcgctatg atctcttcga tggcggcgaa aatcctgtca
      241 cgatccgcag gcctcgcata gcgcatatcg atttgtcccg ttgccgacgg agccaccgtg
      301 ttgacggact gtccaccgga caccaggccg atgttcaacg tcacaccgat ggagagatcg
      361 gtaagcgctg cgatcgccaa ggtcttgtga gcaagtgcgt tgatggcgct gtgccctcgt
      421 tcgaaattgc ccccggaatg cgcggccttt ccgacaacat ccatcaccat gaaaacgccg
      481 cccttgcgct gcgagacgac gttgccgttc ggccttcccg gttcagcatt gtagacggcg
      541 cgcgcgccac gtgccgtatc ttcgatgacc ccgcgcgatt gcggcgaccc gatctcctcg
      601 tcggaggtca gtaatgcgac aagcggcgct gcattgcctc cgaacttctt gaaggccgca
      661 aggacgaacg cgttgagcac cagaccaccc ttcatgtccg tgacgccggg gccatacgcc
      721 cgtccgtccc tgatggtgaa cggacgccgg gccgcctcgc cctttgagaa cacggtatcg
      781 cgatggccca tcagcacgat aggagcattg ccgggtcccg gaattccggc acgaaaaatg
      841 tcgcctgaca cgtcccgcgg cagacgctcg acctcgatgc cgtggctttt gaaaaattcc
      901 tcgaaaactt ccccaacggc gtcgacgccg gccttgtcgt aggatccgct atcgatgttt
      961 acaaccttct cgatcaagct cagcattgca tcgcgctggg aggacaacca ggaacggatc
     1021 tgttcggaca tgtaggcaag gatccttctt tgtgattttg accggcctcg ttctcagaac
     1081 ggtcctcagt cgatccagac tccaataatc aatgccccga tgcaaatcac aaaccacttt
     1141 gcgatcggca gcatcacaaa gcgaacccat tcgccgcacg gaattgtgcc actcgcgagc
     1201 accggcatca acacgccacc tggcacgccc gtttcgacca ggattccggt cacgcgtggc
     1261 tgcgaaaaca actcgcgctc tgtgcaacgg attgagggcg cgcgcggctc aataggccgg
     1321 gtaatgtccg gtttctcgac cagaccgacg tcgcgtggtt tcaatgcggc tgtgaagact
     1381 tcccgtttgc catattcgga aggagcgcgt gatttacccc gatgatgtcc cagggatgaa
     1441 tctcatacct cgtccacacc ccacccttgg catacggatc gttggcagcc aatgcctccg
     1501 cctgctccag cgattgggct tcgtagacga tgatcgcgcc cgcgccatcg ctgaacgggc
     1561 cggcaattac aattttgccc tggctggcga gcccttgcgc atagtcacga tgcaacggtc
     1621 ggacagattg tactttttgc ggatcgggca tgtaggtgat gaagttggcg aatttcatcg
     1681 gctttccctt gcagttgcac ttacgcgggc gaaacgaact cctgcgcggc gccgcgctgc
     1741 ggctggagga acggataatc ggtgtatcct tgctcggcgc cgccgtagaa cgtcggacgg
     1801 tcatatgcat tcaggggcgc gccagtagcc agccgatgcg gcaggtccgg atttgcgatg
     1861 aatgcgcggc caaacgcgat tgcatcgaca atcccggcct tgatggcggc gtcggccgaa
     1921 gcctttgtga agccgcccga cgcgatgagc ggcccctcaa acgattcccg gaacatggct
     1981 gcgacggacg acggcacatt gagatttggc tcctcgcgca accccgcatt cacgcgcggc
     2041 tctatcaggt gcagataggc gatgcggcgg caagatagtg cgccgatggc atgtcggaag
     2101 agcgctgatg gatcggagtc gctgatgtca ttcacggtac tgaacggcga caggcgtacg
     2161 ccgacgcggg aagcgcccag gcggctgacg agcctgtcgg taacctccaa aaggaaccgc
     2221 acccggtttt caacgctgcc gccgtaacga tcggatcggt gattagtccg atcttgcagg
     2281 aactggtcga tcaggtatcc attggcgccg tggagttcaa cgccgtcaaa acctgcaagc
     2341 atcgcgcgcg cggctgcctg atcatactca tcaacaatgc gttggatctc atcgatctcg
     2401 agcgcgcggg gcgtttcata tggcagcct
//
LOCUS       DASWGM010000895         4783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1202310,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000895 DASWGM010000000
VERSION     DASWGM010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202310"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VGA15_24325"
     CDS             <1..466
                     /locus_tag="VGA15_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590854.1"
                     /translation="VQFYYLCLGCLFGGIYLIWRIVNSRFGMVVQGLRSNEQRMQAIG
                     FPAKRYRLVCFVIAGTMCGLAGALLANNTDFVSPAVMYWTRSGDLMVMVILGGMGSLF
                     GPVLGAIVYLLLEEALSQVTEYWALIMGPLLLLIVLFGRGGIMGLLGRLSRG"
     gene            459..1226
                     /locus_tag="VGA15_24330"
     CDS             459..1226
                     /locus_tag="VGA15_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590855.1"
                     /translation="MADPLLRVERLVRRFGGILATDNLSLDVISGELHAIIGPNGAGK
                     TTLISQLTGQLLPNSGTIHFAGQDITRLPAWRRSALGLARSFQITSLLPDFTASDNVA
                     LAAQAHDGHSFRFWGSARGEQHLRQAAQSALARVGLADRADTLVSKLSHGEQRELELA
                     VALATKPQLLLLDEPMAGLGTTESARMVKLLQELRREVTIVLVEHDMEAVFALADRIT
                     VLVYGCVIASDVPAAIRGNEEVRRAYLGDQHVVTRHG"
     gene            1219..1938
                     /locus_tag="VGA15_24335"
     CDS             1219..1938
                     /locus_tag="VGA15_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590856.1"
                     /translation="MADAAPLLEVDGIETCYGLSQVLFGLSLQVRTGEMVAVMGRNGM
                     GKTTSIRSIMGLTPARAGVIRFAGHEVRKLPSFRIAQMGIGLVPEGRQIFPNLTVREN
                     LVAASGNRLGAADAWTIEKIHALFPRLAERGGNMGGTLSGGEQQMLAIGRALMTNPRL
                     LILDEATEGLAPLIREEIWNCLSLLKARGQSVLVIDKNVENLVRIADRHYIIERGRAV
                     WSGTSEQLVAEPDLQHRYLGV"
     gene            complement(1959..3785)
                     /locus_tag="VGA15_24340"
     CDS             complement(1959..3785)
                     /locus_tag="VGA15_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9590857.1"
                     /translation="MISFRSIVLAATILATTGLSAYADKRVALVIGNSAYKNVNRLKN
                     PANDAAAVVAMFKTAGFDSVDLRQDLNVVEMRRALREFGNKTRDADVAVIYYAGHGIE
                     LDGTNYLIPVDATLETDTDVYDETFALDRVLVAVEPAKQLRLVILDACRDNPFAKTMK
                     RTIGSRAIGRGLAKVEPSSPNTMIAFAAKAGSTASDGDGKNSPFALALVDHLAKPGLD
                     LRKAFGFVRDDVLKATSNTQEPFVYGSLGGNDVTLVPAAPAAAASVDQDATMRRDYEF
                     ALQIATKPVWDSFINKYPSGFYTDLAKAQRDKLAAEATRIEATNKAKAAQEEQARLAI
                     EGAKAAEQAKAAEQAKAAEKARVAAELAKKAEEAKVAEAERAKTAAIAKAAEEAKVAE
                     AARVKTAEAAKAAADIKIAPEQRAVDDKVATLTPPADKSDQRTMVDLPRSLQSELRRV
                     GCHTGSIDSNWNAAAQRSLDLFNKHAGMKLDVKLASVDALDAVKSKSTRICPLICDHG
                     FKADGDLCTKITCRSGYEVGDENTCEKIEVRKPTAKREEPQPKREHPVRSNAAAELSK
                     PQALGTGQIICNNQGCRPVAKGCRLGPVKNNYGMSPPGEVCN"
     gene            complement(4204..>4783)
                     /locus_tag="VGA15_24345"
     CDS             complement(4204..>4783)
                     /locus_tag="VGA15_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX9590858.1"
                     /translation="TIQKKLNLHYSLSLDLFGSEVSTNAANAFNAGIKGRYHETQIKD
                     DHRLQNDTYPVLKLVNGEIKHVDEPALTALNMRLRDDYTQDCVKGLLRWNKIISTAGY
                     EFKLTLPNVAFHRQIGEFRDIHATPDGVLVDDATWSKRKGEWLPSVDDGDFIASLMTP
                     VTEIGQFAPWISPPKVGIDNKPGDFEYVKIET"
BASE COUNT          840 a         1409 c         1471 g         1063 t
ORIGIN      
        1 cgtgcagttt tattacctct gcctcggctg tctgtttggc gggatctacc tgatctggcg
       61 catcgtcaat tcgcgcttcg gcatggtggt tcagggcctg cgctccaacg agcagcgcat
      121 gcaggcgatc gggtttcccg ccaaacgtta tcgcctcgtt tgctttgtca tcgccggcac
      181 catgtgtggt ctggccgggg cattgctcgc caacaacacg gacttcgtca gcccggccgt
      241 gatgtactgg acccgctccg gcgatctcat ggtcatggtc atcctcggcg gcatgggctc
      301 gctgttcggc ccggtgctgg gcgcgatcgt ctatctcctg ctggaagagg cgttgtcgca
      361 ggtgacggaa tattgggcgc tgatcatggg tccgctgctg ctgctgatcg tgctgttcgg
      421 acgcggcggc atcatgggtc tgctcgggag gcttagccgt ggctgatccc ttgcttcgcg
      481 ttgaaagact ggtgcggcgg tttggcggga tcctcgccac cgacaatctc tcgctcgatg
      541 tcatcagcgg cgagttgcac gcgatcatcg gcccgaacgg cgccggcaag accaccctga
      601 tcagccaact caccggacag ttgttgccga attccggcac cattcatttt gccggtcagg
      661 acatcacgag actgccagcc tggcggcgca gcgcgctcgg gttggcgcgt tcgttccaga
      721 tcacctcgct gctacccgac ttcaccgcat ccgacaatgt cgcgctggcg gcgcaggctc
      781 acgacgggca ctcatttcgt ttctggggca gcgcaagagg cgaacagcat cttcgtcagg
      841 cggcgcaatc cgcgctggcg cgggttggac tcgctgaccg cgccgacacg ctggtgtcga
      901 agttaagcca tggcgagcag cgcgagttgg agctcgccgt tgccctggcg acgaagccgc
      961 aattgctgct gctcgacgaa ccgatggccg ggctgggcac cacggaatca gcgcgcatgg
     1021 taaagctact gcaggagctg cggcgcgaag tcaccatcgt gctggtcgag cacgacatgg
     1081 aggcggtgtt cgcgctcgcc gaccgcatca ctgttctggt ctacggctgt gtcattgcgt
     1141 ccgacgtgcc ggcggcgatc cgcggcaatg aagaggtcag gcgcgcttat ctcggcgatc
     1201 agcacgtggt gacccgtcat ggctgatgcc gcacccttgc ttgaagtcga tggcatcgag
     1261 acctgttacg gcctgagcca ggtgctgttc ggcctgtcgc tacaggtccg gacaggcgag
     1321 atggtggccg tgatgggccg caacggcatg ggaaagacca cgtcgatcag gtccatcatg
     1381 gggctgacgc cggcgcgcgc gggcgtgatc cgttttgccg gacatgaggt tagaaaactt
     1441 ccctcgtttc ggattgcgca gatgggaatt ggcctggtcc cggagggtcg tcagatattt
     1501 cccaacctca ccgtgcgcga aaatctcgtc gccgcttccg gcaaccgtct gggcgcggcc
     1561 gatgcatgga ccatcgaaaa gatccacgcg ctgttcccgc ggctcgccga acgcggcggc
     1621 aacatgggcg gcacgctgtc cggcggcgag cagcagatgc tggcgatcgg gcgcgcgctg
     1681 atgaccaatc cgcggctgct gatcctggac gaggccaccg agggcctggc gccgctgatc
     1741 cgcgaggaaa tctggaattg cctgtcgctg ctgaaagccc gcggccaatc ggttctggtg
     1801 atcgacaaga acgtcgagaa cctcgtccgc atcgccgacc gccattacat catcgagcgg
     1861 ggacgcgcgg tgtggagcgg cacctcggag cagcttgttg cagagcccga cctgcagcac
     1921 cggtatttgg gcgtgtgagg gcggggcaag tcggtcgctc aattgcaaac ctcaccgggc
     1981 gggctcatcc catagttgtt tttgaccggt ccaagtctac aaccctttgc gactggccga
     2041 catccttggt tgttgcaaat aatttgtccc gttcccaacg cctgcggttt tgataattct
     2101 gctgctgcat tggaacgcac cgggtgctct cgtttgggct gaggttcctc ccgctttgca
     2161 gtcggcttcc tcacttcgat cttttcgcag gtgttttcgt caccaacttc gtatcccgac
     2221 cggcaagtga tcttcgtgca aaggtcgccg tccgccttaa agccgtgatc gcaaatcagc
     2281 ggacaaatgc gcgtcgattt actcttgacg gcatcgagcg catcgacact cgcgagcttc
     2341 acatcgagtt tcatgcccgc gtgcttgttg aagagatcaa gcgaccgctg cgccgccgca
     2401 ttccagttgc tgtcgattga gccggtgtga catcccaccc gtcgcaattc ggattgcagc
     2461 gaacgtggca gatcgaccat cgttcgttga tcggatttgt cagccggtgg cgttaacgta
     2521 gcgaccttgt cgtcgacagc cctttgttca ggggcaatct ttatatctgc ggcagctttg
     2581 gccgcctcgg cggtcttcac gcgtgcggct tccgcaactt tcgcctcttc ggctgccttc
     2641 gctatggcgg ctgtctttgc acgttcggct tcggcaacct ttgcctcttc ggctttcttg
     2701 gcaagctcgg cggcgacacg cgccttttcg gcggccttgg cctgctcggc tgcctttgcc
     2761 tgctcggcgg ctttagcgcc ctcgatggca aggcgcgctt gttcctcctg cgcggccttg
     2821 gccttattgg tggcttcaat cctcgtggct tccgccgcga gcttgtcgcg ctgggctttt
     2881 gcaagatcag tgtagaatcc cgagggatac ttattgatga aggaatccca cacaggcttt
     2941 gtggctattt gcagggcgaa ctcgtagtcg cgccgcatcg tggcatcttg atcgaccgat
     3001 gccgcggcag ccggtgcagc cgggacaagc gtcacgtcgt tgccgcccag cgaaccgtag
     3061 acaaacggct cttgcgtatt gctcgtggcc tttaaaacat cgtcgcgcac gaagccgaaa
     3121 gccttgcgaa gatcgaggcc gggcttggca aggtgatcca ccagcgccaa agcaaatggg
     3181 ctgtttttgc catcgccatc cgatgcggtc gaacctgcct tcgcggcaaa agcaatcatt
     3241 gtgttaggac tggaaggttc gaccttggca agtccgcgtc cgattgccct tgatcctatc
     3301 gtccgcttca tggtcttggc gaacgggttg tcgcggcagg cgtccagaat gacaagccga
     3361 agttgtttag ccggttctac agcgaccaag acgcggtcca gcgcaaaggt ttcgtcgtaa
     3421 acgtcagtgt cggtctcaag cgttgcatcc acgggaatca gatagttggt gccatccaac
     3481 tcgatgccgt gcccggcgta gtagattacc gccacatcgg catcgcgcgt cttgttgcca
     3541 aactcgcgca acgcgcgccg catttcgacg acgttcaggt cttgtcgcaa atctaccgaa
     3601 tcgaagccgg ccgtcttgaa catggcgaca acagccgccg cgtcgttagc cggattcttt
     3661 agccgattta cattcttgta cgcagaattg ccgatcacga gcgccactcg tttgtcggcg
     3721 taggcgctaa ggccggttgt ggccaaaatg gtcgccgcta agacaatcga gcgaaaggaa
     3781 atcatttgct ccgcaaatca cagtcgtatt cgagcgattg tatcttgagg cagcctttct
     3841 gaacagcggg attcagttaa ccgatgggtt aaagtgatct agcgtaaact ttggaaggta
     3901 gccgcggcgt cgcgcttgag ttcgagtcct gtcgcgcggt gtggagcggc acgtccgagc
     3961 aattgatcgc cgagcccgat ctgcagcatc ggtatctggg gaggggctat agtctgattc
     4021 atgaatttgg gattgcaggg tgccgctggc tctatgccac ctctcccctt gtgggggagg
     4081 gttagggagg gggtgagcca caaggacagt gctcgtggtt acccccctct ccaactctcc
     4141 cccacaaggg ggaagagagc cggccgcgct aagttgcaac ggcgcagccg aacgcaaaga
     4201 acctcacgtc tcgatcttca catactcgaa atcgcccggc ttgttgtcga tgccgacctt
     4261 gggcggtgaa atccacgggg cgaattggcc gatttcggtc accggtgtca tcagggaagc
     4321 gatgaaatcg ccgtcatcga cggaagggag ccattcgccc tttcgcttgc tccaggtggc
     4381 atcgtcgacc agcacgccgt ccggcgtggc atggatatcc ctgaactcgc cgatctggcg
     4441 atggaacgcg acattcggca gcgtcagctt gaactcgtag ccggccgttg agatgatctt
     4501 gttccagcgc aacaggccct tgacgcagtc ctgggtgtag tcgtcgcgca gccgcatgtt
     4561 gagtgcggtg agtgccggct cgtcgacgtg cttgatctcg ccgttcacaa gcttgagcac
     4621 gggataggta tcgttctgca ggcggtgatc gtccttgatc tgggtctcgt gatagcggcc
     4681 cttgatgccg gcattgaagg cgttcgccgc attggtggaa acctccgagc cgaacaaatc
     4741 gagcgagagc gagtaatgca gattgagctt cttctgaatg gtc
//
LOCUS       DASWGM010000896         7957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1472506,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000896 DASWGM010000000
VERSION     DASWGM010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7957
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472506"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="VGA15_24350"
     CDS             complement(<1..1670)
                     /locus_tag="VGA15_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9590859.1"
                     /translation="MTDAAATGQKSLRQQAAIARFGGFALRVPDLTKILTEAARACAD
                     GLGVPFSQVWQYRAENNDLIVVAGHGWGDEVVGNAVSCADMRSPQGRAFTTGETSIVG
                     DLQNRNCCGLPPCYAAHGIVSIIDVIIKDDDDQPYGILEIGADQPRDYDGDDIHFLTG
                     FAGVLAEAVASASREAALQVSIDQLKALIEEKDRLLCEKNVADLQLHQAQKMQAVGQL
                     TGGIAHDLNNILTVITGTIEILAEGVADRPELLAIARMIDQAGARGADLTQRLLAFAR
                     KQPLQPREVDVNALVMEAANLLRPMLGELVEVQLMLGADTSRALIDPRQLTSAILNIA
                     LNARDAMANGGKLTIETGNVVLDDSHAAFNSDVTAGKYVMVAVTDSGHGIPAGILEHV
                     FEPFFTTKDVGKGSGLGLSMVYGFVKQSNGHIRIESEEGRGTTVRICLPQAIGVAEPV
                     ELAGTSSIEGGRETILVVEDDHLVRTFVVGQIQSLGYSTLAAVNAAEALVVIDGPQEI
                     DLLFTDMIMPGSMNGRQLADQALQRRASLKILFTSGYSNEAIIHHGHL"
     gene            2373..3290
                     /locus_tag="VGA15_24355"
     CDS             2373..3290
                     /locus_tag="VGA15_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX9590860.1"
                     /translation="MSWLPTNDPVLGDPHSCDALELIIVPRTRDLGDGFEVRRALPHG
                     KRQMVGPFIFFDHFGPVQFMSGKGMDVRPHPHIGLATVTYLFDGSIMHRDSEGNIQEI
                     QPGAMNLMTAGRGIAHSERTPDVQRRDGQKMLGLQSWIALPAGSEEIAPSFQHYAAAD
                     LPTVADSGFTARVIAGSSFGVKSPVAMVSPWFYTEVTAQQGVSVPLDPDHEERAIYII
                     DGEIEIAGDRHEGPRLLIFRPGDRITVKATKPARMMFLGGDALEGPRHIWWNFVSSSK
                     ERIEQAKADWKSGRFAHVPNEHEFIPLPE"
     gene            3358..4287
                     /locus_tag="VGA15_24360"
     CDS             3358..4287
                     /locus_tag="VGA15_24360"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX9590861.1"
                     /translation="MTTMLASDLPLQQIGRGKVRDIYAVGRDRVLLLTTDRISAFDVV
                     MAETIPMKGAVLTQVSAWWFNELEGVVPHHMISADADAIIDEVPALQGHRASLAGRAM
                     LCRRTEVFPIECVIRGYLSGSAWKEYAQAGTLAGEKLAPGLLESAKLEPPIFSPATKA
                     ETGHDENITVARMREILGDEVAYTLESMSRAIYRLGEKLSRDQGIIIADTKFEFGRDG
                     DGRIILIDEVMTPDSSRFWAADAYKPGQPQPSFDKQPLRDYLDAERRAGRWNGDAPPP
                     PLPASVVDATSKRYLEAYRRITGADLEIGGTNR"
     gene            4284..4796
                     /locus_tag="VGA15_24365"
     CDS             4284..4796
                     /locus_tag="VGA15_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9590862.1"
                     /translation="MTLTVREMMNSEVDVIIEYFFKSTPEHLDMLGVDPTRLPTPENW
                     RERFRQDYAQPIEQRARIAVIWLSGDQPIGFSTSDKIRYGEQAHMHLHVTEPERRHQG
                     IGAEGVRRSVEIYFERLKLKRLFCEPNAFNVAPNRALQKAGFKYLKTHMTVPGPLNYH
                     QAVTRWVIER"
     gene            4845..5648
                     /locus_tag="VGA15_24370"
     CDS             4845..5648
                     /locus_tag="VGA15_24370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX9590863.1"
                     /translation="MTDADTVLVERDGSVTIVSINRPHCRNAVDGATARKLFDAFRAF
                     DADAEASVAVFTGTGGTFCAGADLKAVAAGDPNKKREIGGHDSIAPMGPSRLRLSKPV
                     IAAIEGFAVAGGMELALWADMRVVAEDATFGVFCRRFGVPLIDLGTIRLPRLIGHSQA
                     IDLILTGRPVGGTEALRMGLANRLVPKGETRAHAIALAKDIARFPQTCMRADRLSALR
                     QWDLEEEDAIANEMRGGLEVIASGETLSGAARFASGVGRHGAFGGNKVD"
     gene            complement(5820..6902)
                     /locus_tag="VGA15_24375"
     CDS             complement(5820..6902)
                     /locus_tag="VGA15_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEX9590864.1"
                     /translation="MQFLTPDMIAKIASALGLDRNVAQKAIGGAVPALLAGLADVASA
                     PAGARQLTNTLAQQQPGSLESLKSLIGGSGQNTVAETGSSMLSGLFGGGALDTMAQSI
                     GKFAGVDGGSSKSLLGMLGSVVLATLGQQQRSEGLDASGLASLLGSQKDQIAAAIPSG
                     LADQLSAAGLIDRAAGTLRSGTAAASAAGSRIASASSAASSQWPYWLGALVVLGGLAW
                     YALERPARESVAELPRPAATQPATGTVGLAPSDLTIGGVNLANQVNSSVGTLRSVLPT
                     ITDAASAEAALPKLREATTQLNEVSNLAAKLSPEGKSTLVRLIAAAAPTINQMCDKVL
                     ATPGVGDIAKPAIDELRGKLDTLSRA"
     gene            complement(7336..>7957)
                     /locus_tag="VGA15_24380"
     CDS             complement(7336..>7957)
                     /locus_tag="VGA15_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9590865.1"
                     /translation="MVSKYRNMGQTCVCANRIYAQDKIYDQFVEKLSKKVAAMKIGDG
                     TEAGVTQGPLINMAAVDKVEKHIADAVKHGAKIVTGGKRHSLGGSFFEPTVLSNVAPD
                     ALVSQEETFGPLAPVFRFKDEADVIAMCNNSPFGLASYFYSRDLGRVWRVAEALESGM
                     VGVNTGLITTEVAPFGGVKESGLGREGSRHGMDEYVEIKYVMMAGV"
BASE COUNT         1532 a         2514 c         2435 g         1476 t
ORIGIN      
        1 tcgagatggc cgtggtggat aatggcttca ttcgaataac ctgacgtgaa caatattttc
       61 agcgaagccc ttcgctgcag cgcctggtcg gcgagttgac ggccattcat cgaccccggc
      121 atgatcatat cggtgaacaa cagatcgatt tcctgggggc catcgataac gaccaatgct
      181 tccgccgcgt tgaccgccgc caacgtcgaa taccccagac tttgaatttg accgacgacg
      241 aaggttcgca ccagatgatc gtcctcaacg accaggatgg tctcacggcc gccttcgatg
      301 cttgacgtcc ccgccaactc gacaggctcg gcgacgccga tcgcttgcgg cagacagatc
      361 ctcactgtcg tgccgcggcc ttcttcgctt tcgattctga tatgcccatt ggactgtttg
      421 acgaatccat agaccatgct gaggccgagg ccggacccct tgccaacgtc cttggtggtg
      481 aagaagggtt cgaaaacgtg ttccaaaatg ccggcgggaa tgccgtgacc ggaatcagtc
      541 accgcgacca tcacgtactt tccggcggtg acatcgctgt tgaaggcggc atggctgtcg
      601 tcgaggacga cgttgccggt ctcgatggtg agcttgccgc cgttcgccat cgcgtcgcgg
      661 gcgttgagcg cgatattgag gattgcgctc gtgagctgac gcgggtcgat cagcgcgcgc
      721 gacgtatcgg caccgagcat cagctggacc tcgaccagtt cgcccagcat cggacgcagc
      781 aggttggctg cttccatcac cagtgcattg acgtcgacct cgcgcggctg cagcggctgc
      841 ttgcgggcga aggcaagcag ccgctgggtc agatcggcgc cgcgtgcccc ggcttggtcg
      901 atcatcctgg cgatcgccag caattccggc cggtcggcga cgccttccgc caggatttcg
      961 atcgttccgg taatgaccgt cagaatgttg ttgagatcgt gagcgatgcc gccggtcaat
     1021 tgaccaaccg cctgcatttt ctgggcctgg tggagctgca ggtccgcgac gttcttctcg
     1081 cacagcaggc ggtccttttc ctcgataagg gcctttaatt ggtcgatgct gacctgcaac
     1141 gctgcctccc gggaggcaga cgcgacggcc tccgccagga cgccggcgaa gccggtgagg
     1201 aaatggatgt cgtccccgtc gtaatcgcgc ggctgatcgg cgccgatctc aagtatgccg
     1261 taaggctggt catcgtcatc cttgatgatg acgtcgatga tcgagacgat gccgtgcgcg
     1321 gcatagcacg gcggcagtcc gcaacaattc ctgttttgca aatcacccac gatcgaggtt
     1381 tcaccggtcg tgaacgcgcg cccttgtgga gatctcatat cggcgcacga cacggcattg
     1441 ccgactacct catcccccca gccatggccg gcgacgacga taagatcgtt gttctcggcg
     1501 cggtattgcc aaacctggct gaagggcacg ccgaggccgt cggcgcaggc ccgcgccgcc
     1561 tcggtcagga ttttcgtcaa atccggcacg cggagtgcga agccgccgaa gcgggcgatg
     1621 gccgcctgct ggcgcagcga cttctggcct gttgcagcgg cgtcagtcac tggcgcatgg
     1681 tccggcgtgc cgcagtgagg cggcagtctc ctcgcatcgt gggtccgaac cgtccattgc
     1741 gtgttcggca accgaagcca tcagcacccg aatttcctcc ggcgcggaga ttaaatcgct
     1801 gcgacctagc caaatcgtgt ccaagggcaa tagaaacacc ttaggccgat agcgagacca
     1861 cccgaccttc gaatcgcctc ctgaggttgc accttagctc aaccgcggcc ccgtgcaaat
     1921 ttgcaacaca ggaaaccctg catggaaacc gcccaccaag ctgcgttgca ccacgcgtgg
     1981 gacagcgtgt gggcggacga gtggcgcggg cgcgatgggc tgatcgtcga tgatgagcat
     2041 cgtattaccg gcgctgaagc gccggatcaa acgccgagag ttgcacaagc cgccaacgat
     2101 ggcgccgggc gacgtgcggg agcccacgac cgaatctgca agaacacttg ctgtatcact
     2161 ctccggatcg acgcgatgcc aaccgggccc aacggaaaac tgtttcgctg cgcagtgcga
     2221 gcggatcggc agcggcagaa cgctccgcgt catcctgcca attcttcaaa accttgttcg
     2281 ccgggacgac gcggagtttg tgatgactcc gcgcttggac gacactggat aattgctgct
     2341 atgatcacca gcaaaatgac ggagacttcc tcatgagctg gcttcccacc aacgatcccg
     2401 tgctcggcga tccgcactcc tgcgacgcgc tggagctcat catcgtgccg cgcacccgcg
     2461 acctcggcga cggattcgag gtgcgccgcg cgctgccgca cggcaagcgc cagatggtcg
     2521 gtcccttcat cttcttcgat catttcggac cggtgcagtt catgtccggc aagggcatgg
     2581 acgtccgccc gcatccgcat atcgggctcg ccaccgtcac ctatctgttc gacggcagca
     2641 tcatgcaccg cgacagcgag ggcaacatcc aggaaatcca gcccggcgcg atgaacctga
     2701 tgaccgcggg gcggggcatt gcccattcgg aacgcacgcc ggatgtgcag cggcgcgacg
     2761 gccagaagat gctgggcttg cagagctgga tcgcattgcc ggccggctcc gaagaaatcg
     2821 cgccgtcgtt tcagcattac gcggccgccg atttgccgac ggtggcggac agcggtttca
     2881 ccgcgcgcgt cattgcaggc agctcgttcg gcgtgaaatc accggtcgcg atggtgtcgc
     2941 cgtggtttta caccgaggtc acggcgcagc aaggcgtcag cgtcccgctc gaccctgatc
     3001 atgaagaacg tgcgatctac attatcgatg gcgagattga aattgccggc gatcgacatg
     3061 aggggccacg tctgctgatc ttccggcccg gcgatcgcat caccgtcaaa gcgacaaaac
     3121 cggcgcggat gatgtttctc ggcggcgacg cgctggaagg cccgcgtcac atctggtgga
     3181 attttgtctc ctccagcaag gaacggatcg agcaggccaa agcggactgg aagagcggcc
     3241 gtttcgccca cgtccccaac gaacatgagt tcattccgct gccggagtga actgtcccca
     3301 atacctccct ctgccgcttg cgtcgcgcga gatggcgccg tctttgaaag ttgaccgatg
     3361 accaccatgc tcgccagcga cctgcccctg caacagatcg ggcgcggcaa ggtccgcgat
     3421 atctacgccg tcggccgcga ccgcgtgctg ctgctgacca ccgaccgcat cagcgccttc
     3481 gacgtggtca tggccgaaac gatcccgatg aagggcgcgg tactgaccca ggtcagcgcc
     3541 tggtggttca acgagctcga gggcgtggtg ccgcatcaca tgatcagcgc cgatgccgac
     3601 gccatcatcg atgaagtgcc ggcgctgcag ggacatcgcg cctcgctcgc cggtcgcgcg
     3661 atgctgtgcc ggcgcacgga ggtgtttccg atcgaatgcg tgatccgcgg ctatctctcg
     3721 ggctcggcct ggaaggaata cgcgcaagcc ggcacgctgg caggcgagaa gctggcgccg
     3781 ggtctgctcg agagtgcaaa actggagccg ccgatcttca gtccggcgac caaggccgag
     3841 accggccacg acgagaacat cacggttgcg cgcatgcgcg agattctcgg cgacgaggtg
     3901 gcgtacacgc tggagagcat gagccgcgcg atctataggc ttggagaaaa actcagccgc
     3961 gaccagggca tcatcatcgc cgacacaaaa ttcgaattcg gccgcgacgg cgatggacga
     4021 attatcctga tcgacgaggt gatgaccccc gacagttcgc ggttctgggc cgccgacgcc
     4081 tacaagcccg gccaaccgca gccgagcttc gacaagcagc cgctgcgcga ttacctcgac
     4141 gcggaacgcc gcgccggccg ctggaacggc gatgccccgc cgccgccatt gccggccagc
     4201 gtcgtggacg ccaccagcaa gcgttatctg gaggcctatc gccgtatcac cggcgccgat
     4261 cttgagattg gaggaacgaa tagatgacac tgacggttcg ggaaatgatg aattcggagg
     4321 tggatgtcat catcgaatat tttttcaagt ccacgcccga acatctggat atgctcggcg
     4381 tcgaccccac gcgtctgccg accccggaaa actggcgcga acgcttccgc caagattacg
     4441 cacagccgat cgagcagcga gccaggattg ccgtcatctg gttgtccggc gaccagccca
     4501 ttggcttttc gacctccgac aagatccgtt atggcgagca agcccacatg catttgcatg
     4561 tcaccgaacc cgaacgtcgg caccaaggca tcggagccga aggggtgcgg cgcagcgtcg
     4621 agatctattt cgagcggctg aagctgaagc ggcttttctg cgagccgaat gcgttcaacg
     4681 tggcaccgaa tcgcgcgctg cagaaggccg gcttcaagta cctcaagacc cacatgaccg
     4741 tgcccggccc gctcaattat catcaagccg tcacgcgttg ggtgatcgaa cggtgattag
     4801 ctgatccagc cttagaaaaa tagaacaatg agggagcgcc tgacatgacc gatgcagaca
     4861 cagttctcgt cgaacgcgat ggatcggtga ccatcgtttc catcaaccgt ccgcattgcc
     4921 gcaacgccgt cgatggcgcc accgcgcgaa aattgttcga tgcgttcagg gctttcgatg
     4981 ccgatgccga ggcatccgtt gcagtgttca ccggcaccgg cggcactttt tgcgccggcg
     5041 ccgacctcaa ggcggttgca gcgggcgatc ccaacaagaa gcgcgagatc ggcggtcacg
     5101 attcgatcgc accgatggga ccgagccggc tgcgcctgtc gaagccggta atcgccgcga
     5161 tcgaaggttt cgcggttgcc ggcggaatgg aactggcgct gtgggccgac atgcgcgtcg
     5221 tcgccgaaga tgcgactttc ggcgtgttct gccggcgctt cggcgtgccg ttgatcgatc
     5281 tcggcaccat ccggctgccg cggctgatcg gtcactcgca ggcgatcgat ctcattctca
     5341 cggggcgccc ggtcggcggc acggaagcgc tccgcatggg ccttgccaac cgtctcgtgc
     5401 ccaaaggcga aacacgcgcg cacgcgattg cgctggcgaa ggatatcgcg cgtttcccgc
     5461 aaacctgcat gcgcgccgac cggctttccg cattgcggca gtgggacctc gaagaggaag
     5521 acgccatcgc caacgaaatg cgcggcgggc ttgaggtcat cgcctcgggc gagacgctgt
     5581 ccggcgccgc gcgctttgcc tccggcgttg ggcgtcacgg cgcatttggc ggcaacaaag
     5641 tggactaaca cgtcgtcccg gcggaacgcc ggaacccaca acccccggca tccgcgagag
     5701 gcacaggtct cgatttcagt gcccgagccg cgaggccgcg gcgcgcatgc gttcggcttt
     5761 agcaagtacc gccgctgcgc agtctgcctc gagcagaccg cgcaaacggc atctggagat
     5821 caagcgcgcg agagcgtatc gagttttcca cgaagctcat cgatcgccgg cttcgcaata
     5881 tccccaactc cgggggtcgc cagcaccttg tcacacatct ggttgatcgt cggcgctgct
     5941 gccgcgatca gcctgacgag cgtgcttttt ccttcgggac tgagcttcgc cgcgaggttg
     6001 ctgacttcgt tcaattgtgt tgtcgcttcc cgaagcttgg gtagggccgc ctcggcggaa
     6061 gcggcgtccg tgatggttgg aagtacggat ctcaaggtgc cgacggacga gttgacctgg
     6121 ttcgccagat tcactcctcc aattgtcaga tctgacgggg cgagacccac ggttccggtc
     6181 gcgggttggg tcgcagcggg acgcggcagc tcagcaaccg attcacgggc cgggcgctcg
     6241 agggcatacc aggcaagacc acccaatacg accagggcgc ctaaccaata gggccactgc
     6301 gacgacgctg cgcttgaggc gctggcaatt cggcttccgg cggccgaagc tgccgcggtg
     6361 ccgctgcgca gggttcccgc ggctctatcg ataaggccag cagcgctcaa ctgatcggcc
     6421 agaccggacg gaatcgccgc ggcgatctga tccttttgcg agccgagaag cgaagccagc
     6481 ccgcttgcat ccaagccctc gctgcgctgc tgttgaccca gtgtcgcgag gaccacagag
     6541 ccgagcatac cgagcagcga cttgctggaa cccccgtcga ctccggcgaa cttgccgatc
     6601 gactgtgcca tcgtgtccag cgctccgccg ccgaacaggc ccgacagcat gctcgatccg
     6661 gtttccgcga ccgtgttctg gcctgaacct ccgatgaggc ttttgagact ttcgagcgat
     6721 ccgggctgct gctgtgccaa cgtattggtg agttgacgcg ccccagcggg cgcggaggcc
     6781 acgtccgcca gacccgcaag cagcgcgggg acggcacctc caatcgcctt ttgcgcgacg
     6841 ttgcggtcaa ggcccagggc agaagcgatc tttgcgatca tgtcgggggt caggaactgc
     6901 atcacaacag agacgagatt agttgccatg gcgctctctc cagtaaggtt gtgaggtgag
     6961 ttattgggtt agctccgaaa ccgaggtttg gtccgcggat ggtgcaggcg cggctaattt
     7021 ttcggatcga aaattttcac caacagaaaa ttttcaccac cagacttgcg tcgcctgcca
     7081 atcgcccctt gcgattaaac gtgatgtcca agcctgtcct caatcacaac gcggagaggg
     7141 cgcgtacgtt ccagaccgag gaacgtgcgc tggcaagtat atcgatcccg ttgctggcac
     7201 aaagcagacc tgccgcccgc cgccaagagc gtccgcagtc gcagacaaac ccacccgccc
     7261 tgcgcaatca gacatgccaa ggccaggcac gatgatcttg caggtcgccg atttgtgaag
     7321 atgttcgaag agtgcctaga cccccgccat catcacgtat ttgatctcga catattcatc
     7381 catgccgtga cgcgagcctt cgcggccgag accgctttcc ttgacgccgc cgaatggcgc
     7441 cacctcggtg gtgatcaggc cagtattgac cccgaccatg ccggattcca gcgcttcggc
     7501 gacccgccag acccggccga gatcgcgcga atagaaatag gacgcaagac cgaacggcga
     7561 gttgttgcac atcgcgatca cgtcggcctc gtccttgaaa cgaaataccg gcgccaaggg
     7621 cccaaacgtt tcttcctgcg acaccagcgc gtcgggcgcg acgttggaca gcaccgtcgg
     7681 ctcgaagaag ctgccgccaa gcgaatggcg ttttccgccg gtgacgatct tggcgccgtg
     7741 cttgacggcg tccgcaatgt gcttttcgac cttgtcgacc gcggccatgt tgatcaacgg
     7801 gccctgcgtc acgccggctt cggtgccgtc gccgatcttc atcgccgcga ctttcttgga
     7861 aagtttttcc acgaactgat cgtagatctt gtcttgcgca tagatgcggt tggcgcagac
     7921 gcaggtctgg cccatgttgc ggtatttcga aaccatg
//
LOCUS       DASWGM010000897         4730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1472646,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000897 DASWGM010000000
VERSION     DASWGM010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472646"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /locus_tag="VGA15_24385"
     CDS             complement(<1..1662)
                     /locus_tag="VGA15_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9590866.1"
                     /translation="MIPDRIAFCASDTNLSRRSFLGTSAAIGGGLMLSLTLPLGRSEA
                     ASSDSFAPNAFIRIGSDSQVVLTMPYVEMGQGTYTSIPMLIAEELDVSLKQVWLEHAP
                     PNEKLYANPLLGVQATGNSNAMRGAWKPLREAGATARIMLVAAATKRWGVDAKSCRTE
                     DGEVIHVPTGRRLKYGEVVADAANMPVPGNVTLKSPGDFKLIGTRAKRLDAPEKVNGT
                     ALYGIDARPAGVKIATLAQSPVFGGRVKSVDDTAAKAVKGVRQIVRLDDAVAVVADHM
                     GAAKKGLAALKIEWDDGQHAKLATEEIARKLEQATLGSGAVAQNIGDADKAMASATTK
                     VEASYQVPFLAHATMEPMNCTVHFRKDECEIWIGNQAVARVQAMAAKEAGLSPDKVII
                     RNHLIGGGFGRRLEADGAVRAVQIAKHVDGPVKVVWTREEDIQHDMYRPYWFDRISAG
                     LDKQGKPVAWKNRFAGSSVIARWLPPAFKDGLDPDSTEGAIDLVYDLPNFHVEYVRAE
                     PPGIPTAFWRSVGPSHNVFVTESFIDELAAAAKQDAVAYRRALLGK"
     gene            complement(1665..2135)
                     /locus_tag="VGA15_24390"
     CDS             complement(1665..2135)
                     /locus_tag="VGA15_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9590867.1"
                     /translation="MSFAIKVNDNTHSVDVDGDTPLLWVLRDVLGMTGTKFGCGIAQC
                     GACTVHIDGIATRSCVTSIESLGKSQITTIEAIGNTPAGAKIQKAWLDHEVVQCGYCQ
                     SGQIMSASALLASNPHPSNADIDDAMSGNICRCGTYVRIREAINLAAQTNEKRG"
     gene            complement(2170..3000)
                     /locus_tag="VGA15_24395"
     CDS             complement(2170..3000)
                     /locus_tag="VGA15_24395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9590868.1"
                     /translation="MSFVKVGDENSTPIEIYYEDHGSGPPVVLIHGWPLNGDAWEKQV
                     SALLAAGHRVITYDRRGFGRSSKPGIGYNYDTFASDLNILLNTLNLTDVSLVGHSMGT
                     GEITRYIGKYGTKRLRKTVLIGTLGPYLLKAADNPDGVPASVFDEVRAGIKADRPAAV
                     MEFLKNFYSVGGPDGKLVSERVIEANWTVAMGASPIGTLACVDSWVEDFRKDIARNDV
                     PTMIIHGDNDRILPADATSRRQAKLIKNVKYVEIKGGSHGITWTRAEEINAALVGFLA
                     "
     gene            complement(3263..4111)
                     /locus_tag="VGA15_24400"
     CDS             complement(3263..4111)
                     /locus_tag="VGA15_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9590869.1"
                     /translation="MNRFEKSTTARQIRLERRLPSYWRVTFDHPPLNIFGPETIPQLN
                     ETIVALETDEHVKVVVFDSAVEGFFLTHYNFLAPLEDSTSLPPGPTGLQALPDMLVRL
                     SRAPVVSIVSIRGRATGVGSELALASDMRFASSEKAILSQWEVGAGLVPGGGPMARLP
                     RLIGRGRALEVLLGADDVRGELAERYGYVNRSLPDSELDAFVDALARRIASFDKGAIA
                     DTKRLVDVASLPSDAEISLEWDAFLASLDRPASQLRIKALMERGFHRPGDVEDRLGYH
                     VGQLGG"
     gene            complement(4108..4341)
                     /locus_tag="VGA15_24405"
     CDS             complement(4108..4341)
                     /locus_tag="VGA15_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590870.1"
                     /translation="MVPGLIVATDKGFSSNRFAWEHQFHKLDLKVELNEAVVNQLHST
                     QLLKSSHPVMLAALDVGARVARVAIPDNQGVRQ"
BASE COUNT          907 a         1430 c         1396 g          997 t
ORIGIN      
        1 cttgccgagc agcgccctac gataggcgac cgcgtcctgc ttggcggctg ccgccaactc
       61 gtcgatgaag ctttcggtga cgaagacatt gtgcgagggg ccgacactgc gccagaatgc
      121 cgtcggaatg cccggaggct cggcgcggac atattcgacg tgaaaattcg gcagatcgta
      181 aacgagatcg atcgcgcctt ccgtcgagtc gggatcaagt ccgtccttga aggccggcgg
      241 cagccatcgc gcaatcaccg aagagccagc gaagcgattt ttccaggcca ccggcttgcc
      301 ctgcttgtcc agtccggcgg aaatgcgatc aaaccaatac ggccgataca tatcgtgctg
      361 gatgtcttcc tcacgcgtcc atacgacctt gacggggccg tcgacatgct tggcgatctg
      421 aacagcccgg actgcgccgt cggcttccag ccggcgaccg aagccgccgc caatcagatg
      481 attgcggatg atgactttat ctggcgacag gcccgcttcc ttcgcggcca tcgcctggac
      541 gcgtgccacg gcctggttgc cgatccagat ctcgcactca tccttgcgaa agtgcaccgt
      601 gcaattcatc ggctccatcg ttgcgtgcgc gaggaagggt acctgatagc ttgcctcaac
      661 cttggtggtg gcactcgcca tggccttgtc ggcatcaccg atattctgcg cgacagcgcc
      721 tgaaccgagc gtcgcctgtt cgagcttgcg cgcgatctct tcggtggcga gttttgcgtg
      781 ctgaccgtcg tcccattcga tcttgagcgc tgccagacct tttttcgcag ctcccatgtg
      841 atcggccacg accgcaacag catcgtcaag ccgaacgatc tggcgcacgc ccttcaccgc
      901 ctttgcagcc gtgtcgtcga cactttttac gcgcccgcca aaaacgggcg attgcgccag
      961 cgtcgcgatc ttcacgccgg ccgggcgcgc gtcgataccg tagagcgcgg tcccgttgac
     1021 cttctccggt gcatcgagcc gcttggcccg cgtgccgatg agcttgaaat ctcccgggga
     1081 cttcagcgtg acgtttccag gaacgggcat gtttgctgcg tcagcgacaa cctcaccgta
     1141 tttcaggcgg cgaccggtcg gcacgtggat cacctcgccg tcctctgtgc ggcatgattt
     1201 tgcatcgacg ccccaacgct tggtagccgc cgcgaccagc attatccttg cggtggcgcc
     1261 cgcttcgcgc aacggcttcc acgcgccgcg catcgcgttt gaattgccgg tcgcctgaac
     1321 gccgagcagc gggttggcat agagcttctc gttcggtggt gcgtgctcga gccacacctg
     1381 tttcaggctc acatcgagct cttcggcgat cagcatcggg atcgatgtgt aagtgccttg
     1441 ccccatttcg acatagggca tggtcaggac gacctggctg tcgctgccga tacgaataaa
     1501 ggcgttcggt gcaaaactat cggaactggc agcttcgctt cggccgagag gcagggtcag
     1561 gctcagcatc aggccgccgc caatggctgc gctggtgccc agaaagctgc gccgcgacag
     1621 gttagtgtca gaagcgcaaa aagcaatgcg atcagggatc atggtcagcc tcgtttttca
     1681 ttcgtctgcg cggcgaggtt gatcgcttcg cggatacgca cataggtgcc gcagcggcag
     1741 atgttgccgc tcatggcgtc atcgatgtcg gcattgctcg gatgtggatt gcttgccaga
     1801 agcgccgaag ccgacatgat ctgtcccgac tggcagtagc cgcactggac gacctcatgg
     1861 tcaagccagg ctttctggat cttggcgcct gccggcgtgt tgccgatcgc ctcgattgtc
     1921 gtgatctgcg atttgccgag actttcgatg ctggtgacgc aggaacgcgt cgcgatgcca
     1981 tcaatatgta cggtgcacgc gccgcactgc gcgatgccgc acccgaattt ggtgccggtc
     2041 ataccgagta cgtctcgcag cacccatagc agcggcgtgt cgccgtcgac gtcgacactg
     2101 tgggtattat cgttgacttt gattgcaaaa ctcatgatca cctttcgaac gatgtgaaaa
     2161 gaccgcttgc tatgcgagga atcccacgag cgcggcgttg atttcctccg cgcgcgtcca
     2221 cgttatgccg tgcgatccgc ctttgatttc cacgtacttg acgttcttaa tgagcttcgc
     2281 ttgccgccga gacgttgcat cagccggtag aatgcgatca ttatcgccgt gtatgatcat
     2341 tgtcggcacg tcgttgcgtg cgatgtcctt ccgaaagtcc tcaacccaag agtcgacgca
     2401 agccaacgtg ccgatgggtg acgcgcccat tgcgaccgtc caattcgcct caatcacgcg
     2461 ttcgctgact agctttccgt ccgggccgcc aacgctgtag aaattcttta ggaactccat
     2521 tactgcggcg ggccggtcgg ctttgatccc ggcacggacc tcatcgaata cacttgctgg
     2581 cacgccgtcc ggattgtccg ccgcctttaa caggtagggc ccaagcgtac cgatcagcac
     2641 ggtcttccgc agccgcttcg tgccatactt gccgatgtag cgggtaatct cgcccgtccc
     2701 catggagtgt ccgaccagcg agacatcggt gagattcagt gtgttcaaca agatgttgag
     2761 gtcggacgcg aaggtatcgt agttgtatcc gatgcctggc ttgcttgacc ggccaaagcc
     2821 gcgtcgatcg tatgtgatga cccgatggcc ggcggccaac aacgcgctga cctgcttttc
     2881 ccacgcgtcg ccgttcaaag gccaaccgtg aatgaggacg accggcgggc ccgaaccatg
     2941 atcctcgtag tagatctcga tgggcgtgga attttcgtct ccaaccttga cgaaactcat
     3001 gggcttactc ctattgttat tgtcgtggcc gatgcttgag gtctctgccc cctgtgccgc
     3061 gtttacgaat gctggcagca gcggcgcggc aagagtcgct gtggcaagcg aaagggcgtc
     3121 gcgcctggaa aagccttccg gtggcgccga tggtcggccg ccttccttct ggggtcgttc
     3181 agtcataatg tctccttgtt gcgagaggcg gcgcgcacgc gcgacgtgcg ccgttcgttt
     3241 ggaagcgacc ggcgggatct tctcagccgc ctagctggcc gacatgatat ccgagccgat
     3301 cttcgacatc gccggggcga tggaagccgc gctccatcag cgccttgatt ctgagctggc
     3361 tggcggggcg gtccagcgag gcgaggaaag cgtcccattc aagtgagatt tccgcgtcgg
     3421 acggcaggct tgctacgtca accaaacgct tggtgtcagc gatcgccccc ttgtcgaacg
     3481 atgcgatccg tcttgccagc gcatcgacaa acgcatcgag ttcggagtcc ggcagggatc
     3541 gattgacgta accgtaacgc tcggcgagtt cgccgcgaac atcgtcagca ccgagcagca
     3601 cttcgagcgc acgccctcgg ccgatcaggc gaggcagccg agccatcggt ccgccgccag
     3661 gcaccaggcc ggcaccaact tcccattgcg acagaatggc tttctcgctg ctcgcaaaac
     3721 gcatgtcgct ggccagcgca agctcgcttc cgaccccggt cgcacggccg cggatcgaga
     3781 caatggatac gacgggagcc cggctgaggc gtaccagcat gtcgggaaga gcctgcaagc
     3841 ccgtgggtcc gggaggcagg cttgtcgagt cctcgagcgg cgcgaggaag ttatagtgcg
     3901 tgaggaagaa gccttcgacg gcgctatcga agacaacgac cttcacatgc tcatcggttt
     3961 cgagcgccac gatggtctca ttgagctgcg ggatcgtttc gggtccgaag atattaagcg
     4021 gcggatgatc gaacgtaacg cgccaataag acggcaagcg ccgctcaagc cggatttgtc
     4081 gggccgtggt tgatttttcg aagcgattca ctggcgtacc ccttggttgt ctggaatagc
     4141 gacgcgcgca acgcgcgcgc cgacatccaa agcagcaagc atgaccggat gactgctttt
     4201 caataattga gtcgagtgca actggttcac gacggcttca ttcagttcga ctttcaaatc
     4261 gagcttatga aattgatgtt cccaagcgaa gcgatttgaa ctgaacccct tatctgtcgc
     4321 gacgataaga ccaggaacca agctctgcca ccgtcaatca gcggcgctga atcgatccgg
     4381 tgtggccatg ctacgcaatc agcagaacct gcgaacgcgg agtaaacagt ccgccaatct
     4441 ccggagaaga tatggctatc tcgaaaaagc gtttgatgac gacaccagtc actgcctagc
     4501 gtgaaacggc tcgatcgcaa acgtgcgagg cagatacgct aatgcaggga tgcgaatttg
     4561 tacaaagcaa ctgaagttgt tgcgttgcgc gttaccgcca acagatcggg agtcctccga
     4621 ttccggttgg tgctcgcgac gatcgtttca tacttggcga tgatggcctt tttgatccac
     4681 cgcgcgcggc acgcgacgca aactgccgtt gttgcagctg acttcagccc
//
LOCUS       DASWGM010000898         5354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2193865,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000898 DASWGM010000000
VERSION     DASWGM010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2193865"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..586)
                     /locus_tag="VGA15_24410"
     CDS             complement(236..586)
                     /locus_tag="VGA15_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590871.1"
                     /translation="MRDVLLQCAGIGAIAVALIHGVLGETKVFARATIEPPRLRNLIR
                     LVWQAGTVAWIGGGVLLIAAPWMASESARHWIVVTLAAVFGFAALANAWALRGRHFGW
                     VALSAVVALAVAGY"
     gene            683..1219
                     /locus_tag="VGA15_24415"
     CDS             683..1219
                     /locus_tag="VGA15_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9590872.1"
                     /translation="MADQLSAKDWLDQGLRALARSGFTALKAEPLAKAMGVSRGSFYW
                     HFVDIGAYHAAILKHWREVAAEQIIANLEAASGDDDPVQLLLRRAFGTKPALENAIRT
                     WATLDPAARSAVQAIDRRRLGYVEKLLSTSGLPADVARPRAQILYWAFLGFALSDRPL
                     PQARQAEVVDELLRMAAR"
     gene            1287..1484
                     /locus_tag="VGA15_24420"
     CDS             1287..1484
                     /locus_tag="VGA15_24420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX9590873.1"
                     /translation="MTSSPERLEGTVDPKLLEILVCPLTKGPLEFDATRQELISRSAK
                     LAYPIRDGIPIMLPEEARKID"
     gene            1477..2424
                     /locus_tag="VGA15_24425"
     CDS             1477..2424
                     /locus_tag="VGA15_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9590874.1"
                     /translation="MISPLRAAAIIGALAILDLFGMTLNASAEETKIRVGALDAALTV
                     PADVERPPVALLIAGSGSTDHDGNGPQIKPATLKKLSEQFAARKIATLRYDKRGAGGW
                     QAEFGRPDDFRFKDYVEDAAALVNYLRGSGKFSRVILVGHSEGGLVAILTARRVPVDR
                     VVLLATAARRQGDLLKAQLEKTLAPDAFKPIAKAIDDIMAGQIVDPPPGLAIPPSMQP
                     GIASAFAEDPIGPLKLIEQPTLIVAGGRDRQVARLDFVALGAASPAAKTLWLPDMNHV
                     LVDVADEADDVAAYNQPEHALDTVLIDSVANFVLANDVR"
     gene            complement(2473..3693)
                     /locus_tag="VGA15_24430"
     CDS             complement(2473..3693)
                     /locus_tag="VGA15_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis hydroxylase"
                     /protein_id="HEX9590875.1"
                     /translation="MSAQRSIVIGGGALAGLALALALRQGLGADIPIIVADPALGLRP
                     SRDPRATAIVAACRRLFDAIGVWDQVATEAQPILDMVVTDSRLEDATRPVFLTFAGDV
                     EPGEPFAHMVENRHLIDALVARAEGQGVDLRATAVTSFDARSDGVDVTLADGGVIAAS
                     LLVAADGARSKLRERAGIATHGWDYDQSGIVVTVGHERDHHGRAEEHFLPAGPFAILP
                     LTGQRSSLVWTEKRGEAARIVELSEEEFHGELEQRFGLHLGEIKALDKPRAFPLGYFV
                     ARSFIGERMALIGDAAHVIHPIAGQGLNMGLKDVAALAEVVVDAARLGMDLGQADVLD
                     RYQRWRRFDTMAMGLATNSLNLLFSNKSTLLRTVRDIGLGLVDRAPPLKSLFIRQAAG
                     LAGEVPRLLKGEAL"
     gene            3900..4760
                     /gene="tesB"
                     /locus_tag="VGA15_24435"
     CDS             3900..4760
                     /gene="tesB"
                     /locus_tag="VGA15_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX9590876.1"
                     /translation="MSKGLIDLISILDLEPIEVNLFRGSSPKTSWQRVFGGQVIGQAM
                     VAACRTVEGRLPHSLHCYFILPGDPQIPIIYEVERLRDGKSYSTRRVTAIQHGNAIFS
                     IMVSFHVEEQGSFDHQDKMPDVPPPEKLTAEEVAKQPMFREMPDFIRRYYESDRPIEL
                     RPVELGRYFGQKIDDGRIHVWIRTASKLPDDPALHMCALAYASDFSLLDAAMARYGRT
                     LFDKRMMPASLDHAMWFHRSFRADEWLLYAQDSPSAQGGRGLTRGMIFKQDGTLVASV
                     AQEGSLRERK"
     gene            complement(4781..>5354)
                     /locus_tag="VGA15_24440"
     CDS             complement(4781..>5354)
                     /locus_tag="VGA15_24440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590877.1"
                     /translation="IGFLLVIPAPWVATSFYRWIISRLRVPQRPNLAFTGQVGDIWYV
                     FGALALLSYAGLTGVRYLRLILIPVQGFLSWMTVRWIAANISSNGRQLPIAFKGSALT
                     YVGWYILMYLSFITIIGWAWVITAWMRWICRNIEGTQREILFIATGLEMLWRTLVFAV
                     GAALIIPIPWVMRWYTTWYVSQFALVERAP"
BASE COUNT         1059 a         1718 c         1628 g          949 t
ORIGIN      
        1 cgacatagcg ggtaaaacgc aattcaatct ctttcgctcc gtggagaccc atcccagccc
       61 ccttgtttca gtgggattcg cctccataaa tgattctcaa atacccgaaa ctgcggcttt
      121 ctgactcagt agtactagag ccttttccgt ttcgatagaa tcgaaacggg gctctagatt
      181 cttgatttga cgcgtttctt gacgcgaacc ggtctccact tcgcttgaaa acgctctaat
      241 atcccgccac ggccagcgcg accacggcgc tcagcgccac ccacccgaaa tgccggccgc
      301 gcagcgccca ggcattggcc agcgcggcaa acccaaacac tgcggcgagg gttacgacaa
      361 tccagtgccg cgccgactcc gacgccatcc agggtgcggc gatcaacagc acgccgccac
      421 cgatccatgc cacggttccg gcctgccaga ccagacggat cagattgcgc agcctcggag
      481 gctcgatggt ggcgcgcgca aacacttttg tttctcccag cacgccgtga atcagcgcta
      541 ccgcgatcgc gccgatccca gcgcactgca acaaaacatc gcgcatcgga tatcctccat
      601 acatgactgt atggtagcta tggcggagac gctggcgccg gtcaatacag ttgtgtatgg
      661 tcaagttgtc gcagggatcc gcatggccga tcaactttcc gcaaaggact ggctcgatca
      721 aggcctcagg gcgctggcga gaagcggttt cacggcgctg aaggccgagc cgctggcgaa
      781 agcgatgggc gtttcgcgcg gcagcttcta ctggcacttc gtcgacatcg gcgcctacca
      841 cgccgccatc ctcaagcatt ggcgcgaggt cgccgccgag cagatcatcg ccaacctcga
      901 ggccgcgtct ggcgacgacg acccggtgca gctcttgctg cgccgggcgt tcgggaccaa
      961 gccggcgctg gagaacgcga tccgcacctg ggctacgctc gatcccgcgg ctcgaagcgc
     1021 cgtgcaggcc atcgaccgtc gccggctggg ctatgtggag aaactcctca gtacgtccgg
     1081 attgccggcg gatgtggccc gtccccgggc gcaaattctt tactgggcgt ttctcggctt
     1141 tgcgctgtcg gacaggccgt tgccgcaggc acggcaagcc gaggtggtcg acgagctgct
     1201 ccggatggcc gcgcggtgag cgccggcggc tacacgtcat gatcgaatat gctacagcgg
     1261 gtcgaattgc caagtgggag cagcgcatga catcctcacc cgaacgcctt gaaggcaccg
     1321 tcgatccgaa attgctcgaa attctggtct gtccgctgac caagggcccg ctggagtttg
     1381 acgccacaag gcaggagctg atctcgcgtt ccgcaaaact ggcctatccg atccgcgacg
     1441 gcattccgat catgctgccg gaagaggcgc ggaagattga ttagcccctt gcgcgccgcg
     1501 gcgatcattg gtgcgctcgc aatactcgac ttgttcggca tgaccctgaa cgcatccgct
     1561 gaagaaacca aaatccgcgt cggcgccctc gacgcggcgc tgaccgttcc ggccgatgtg
     1621 gaacggccgc cggtcgcgct tttgatcgcg ggctccggat ccaccgacca tgacggcaac
     1681 ggaccgcaga tcaaacccgc aaccctgaag aaactctccg agcaattcgc cgcgcgcaaa
     1741 atcgcaacgc ttcgctacga caagcgcggc gcgggcggat ggcaagcgga atttggacgg
     1801 ccggatgatt ttcgtttcaa ggactatgtc gaagatgccg cggcgctggt gaattatctg
     1861 cgcggcagcg gaaaattttc cagggttatt ctggtcggac acagcgaggg cggcctggtc
     1921 gcaatcctca ccgcccggcg cgtgccggtc gaccgtgttg tcctgctggc caccgcagcg
     1981 cggcgacagg gcgacctgct caaggcccaa cttgaaaaga cgcttgctcc ggacgcgttc
     2041 aagccgatcg cgaaggcgat cgacgacatc atggccgggc aaattgtcga tccgccgccg
     2101 gggctggcga tcccgccgtc gatgcagccc ggcatcgcct cggccttcgc ggaagacccg
     2161 atcggcccct tgaaactgat cgaacagccg accctgattg tcgcgggcgg ccgcgaccgg
     2221 caggtggcac gactggattt cgttgccctc ggcgcagcgt caccggcggc gaaaacgctt
     2281 tggcttccgg acatgaatca cgttctggtc gatgttgcag atgaagcgga tgatgtggcc
     2341 gcctataacc aacctgagca cgcgctcgac actgtcctga tcgattcggt cgcgaatttc
     2401 gttcttgcga acgacgtccg ttgatctttg ctgtagtcgt tccggcgcac gccgggagga
     2461 cgctgatgat tgctacagcg cctcgccctt caacagccgc ggcacctcgc cggcaagccc
     2521 cgccgcctga cggatgaaca ggcttttcag cggcggcgcg cgatcgacca gcccgagccc
     2581 aatatcgcgc accgtgcgta aaagcgtcga tttgttcgag aacagcagat tcagcgaatt
     2641 ggtggcgagc cccatcgcca tggtgtcgaa gcgtcgccaa cgctgatagc ggtcgagcac
     2701 atcggcctgc ccgagatcca tgccgagccg cgcggcatcg acgaccactt cggcgagtgc
     2761 agcgacgtcc ttgaggccca tgttgagacc ttggcctgcg atcgggtgaa tcacatgcgc
     2821 ggcgtcgccg atcagcgcca tccgttcgcc gatgaacgag cgcgccacga agtagccgag
     2881 tggaaacgcg cgcggtttgt cgagcgcctt gatctcaccg agatgcagcc caaaacgctg
     2941 ttcgagttcg ccgtgaaatt cttcttcgct aagctcgacg atgcgcgccg cctcgccgcg
     3001 cttctcggtc cacaccagcg atgagcgttg tccggtcaag ggcaggattg cgaacggccc
     3061 ggcgggcagg aaatgttcct cggcccgacc gtgatgatcg cgctcatggc cgacggtgac
     3121 gacaatgccg gactgatcgt aatcccagcc atgggtggcg atcccggcac gctcgcgcag
     3181 tttcgagcgc gcgccgtcgg ccgccaccag caggcttgcc gcaataacgc cgccgtcggc
     3241 gagcgttaca tccaccccat cgctgcgcgc atcgaagctc gtgaccgcgg tggctctgag
     3301 atcgacgccc tggccttccg cgcgcgcgac caacgcatcg atcagatgac gattttcgac
     3361 catgtgcgcg aacggttcgc ccggctcgac atcccccgca aaggtcagga acaccggacg
     3421 ggtggcgtcc tccagccggg aatcggtcac gaccatgtcg aggatcggct gggcctcggt
     3481 ggcgacctga tcccacactc cgatggcatc gaacagccgg cggcaggcgg cgacaatggc
     3541 ggtggcacgc ggatcgcggc ttggccgcag ccccagcgcc ggatcggcaa cgatgatcgg
     3601 tatatcagcg ccgaggccct gacgcagcgc cagcgccagc gccagccccg ccaatgcgcc
     3661 gccgccaatg acaatgcttc gctgtgccga catgctaaac cttactcacg gagctcatag
     3721 actacacttg ctacatgact atagctgggc gaaacaggta ggcgaagcaa ggctcggcgg
     3781 caatccaggt caggtcgtca ttccggggca cgcgtcttcg cgtgaacccg gaatctcgag
     3841 attccgggtt cgaggccgtc ggcctcgccc cggaatgacc agtaaccgaa agcgcttcga
     3901 tgtccaaagg cctgattgac ctgatttcca ttctcgatct cgagccgatc gaggtgaact
     3961 tgtttcgcgg ctccagcccg aagacgagct ggcagcgggt attcggcggg caggtgatcg
     4021 ggcaggcgat ggtggcggcg tgccgcaccg tcgaagggcg cctgccgcat tcgctgcact
     4081 gctatttcat cctgccgggc gatccgcaga ttccgatcat ctacgaggtc gagcgcttgc
     4141 gtgacggcaa gagctattcg acccgaaggg tcaccgcgat ccagcacggc aatgcgatct
     4201 tttcgatcat ggtgtcgttt catgtcgagg agcaaggctc gttcgaccac caggacaaga
     4261 tgccggacgt gccgccgccg gaaaaactca ccgccgaaga ggttgcgaaa cagccgatgt
     4321 tccgcgagat gccggatttc atccgccgct attacgaatc cgaccgcccg atcgagcttc
     4381 gcccggtcga actcggccgt tatttcggcc agaagatcga cgacggccgc attcacgtct
     4441 ggatccggac cgcgtcgaaa ctgcctgacg atccggcgct gcatatgtgc gcgctggcct
     4501 atgcatcgga cttctcgctg ctcgatgcgg cgatggcgcg ctacggccgc accctgttcg
     4561 acaaacgcat gatgccggct agtctcgatc acgcgatgtg gtttcatcgg tcgttccgtg
     4621 ccgacgaatg gctgctttac gcgcaggact cgccgagcgc gcagggcggg cgcggtctga
     4681 cccgcggcat gatcttcaaa caggacggca cgctggtggc ctcggtggcg caggaaggct
     4741 cgctgcgcga gcgcaaataa caacgccgcc gcttgttgcg ttacggagct ctttcaacga
     4801 gcgcaaattg cgacacgtac caggtggtgt accagcgcat cacccaggga atcggaatga
     4861 tgagcgccgc gcccacggcg aacaccaggg tccgccacag catttccaat cctgtcgcaa
     4921 tgaacaagat ctcgcgctgg gtgccctcga tgttacggca gatccatcgc atccatgcgg
     4981 tgatcaccca tgcccagcca atgatggtga tgaaagacag atacatcagg atataccagc
     5041 cgacataggt caacgcgctg cccttgaagg cgatcggaag ctgacgtccg ttcgaactga
     5101 tattcgcagc gatccatcgc accgtcatcc aggacagaaa gccctgaacc ggaatgagaa
     5161 taagtcggag gtagcggacg ccggtcaatc cggcatacga caaaagcgcc aacgcgccga
     5221 agacgtacca gatgtcgccg acttggccgg tgaaggcgag gttgggccgc tgcggcacac
     5281 ggaggcggga aatgatccag cggtagaagc tcgtcgccac ccagggcgcc gggatcacca
     5341 gcagaaatcc gatc
//
LOCUS       DASWGM010000899         6107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_225908,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000899 DASWGM010000000
VERSION     DASWGM010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225908"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VGA15_24445"
     CDS             <1..560
                     /locus_tag="VGA15_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9590878.1"
                     /translation="RERGFHSAKMSPCKLSARTGTGRIAYDFVHAAFGEAEKLSPYNA
                     IGVADSLIDLLLLPLRQAGTPLDCSGPVAMYVRAQAFIREHLRDPDLSIDQISAALGC
                     TKRYLHMLFSDRGMTVSDYIWQARLQNCRQELEAQGGKTITDVAFSWGFSSSSHFSRV
                     FRKYFGIVPSSIHKAQHGSLSPDLP"
     gene            complement(692..2314)
                     /locus_tag="VGA15_24450"
     CDS             complement(692..2314)
                     /locus_tag="VGA15_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9590879.1"
                     /translation="MIRLDNVSKQIGHQILFIEASAALQKGEKVGLVGPNGAGKTTLF
                     RMITGEESPDEGQVAVDRGVTIGYFSQDVGEMSGRSAVSEVMDGAGPVSAVASELKEL
                     EAAMGDPDRADGMDDIIERYGEVQARFEELDGYALEGRAREVLAGLSFSQEMTDGDVG
                     ALSGGWKMRVALARILLMRPDAMLLDEPSNHLDLESLIWLEQFLKGYEGALLMTSHDR
                     EFMNRIIGKVIEIDGGALTTYSGNYEFYEQQRALSEKQQQAQFERQQAMLAKEINFIE
                     RFKARASHAAQVQSRVKKLEKIERVEPPRRRQMVSFDFMPAPRSGEDVVSLKNVHKGY
                     GVRRIYQGLDFMVSRRERWCVMGINGAGKSTLLKLVAGSTQPDDGTVALGGSVKMGYF
                     AQHAMDLLDGERTVFQSLEDSFPQAGQGSLRALAGCFGFSGDDVEKKCRVLSGGEKAR
                     LVMAKMLYDPPNFLVLDEPTNHLDMATKQMLITALSQYEGTMLFVSHDRHFLAALSNR
                     VLELTPEGIHQYGGGYTEYVARTGQEAPGLRS"
     gene            complement(2483..3670)
                     /locus_tag="VGA15_24455"
     CDS             complement(2483..3670)
                     /locus_tag="VGA15_24455"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9590880.1"
                     /translation="MMDRRQFICAGAAGAALLANVPEAYAATYDLVIKGGRVIDPSVG
                     LDAERDVAIMRGRIAAVEANIAADAAETIDARGKIVAPGLIDIHTHAGRSKEGPPMCL
                     QDGVTCWVDVGSGGADTIDQVAAVARGAPQIGRALVNIARTGVISPGGELHDLNSANV
                     ALAQGAIARNHDVVVGVKARLSNNVAGANDLEALRRAQEAAAPFNLPVMIHVGQNYSP
                     MRAILALLKRGDIVTHMYAPGMNGILDDKGVLFPEVTAARRRGVLFDFGNGVADHFDW
                     DTVEKATRQGLWPDTFSTDWSVTSKTTGVVDLPNVMSKFIMFGMPLSQIIACATVNAA
                     RVFPSFDDRGTLNVGAPADVAIMELREGAFEFLDNYKGIRAGRQRLFPAGTVLAGKNV
                     QRA"
     gene            complement(4145..4654)
                     /locus_tag="VGA15_24460"
     CDS             complement(4145..4654)
                     /locus_tag="VGA15_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEX9590881.1"
                     /translation="MHPHLKMPLFSLVALATLLSGEVNAQVPSAIAVPGQYIVTTLHA
                     EGAQVYECTVGPDKKLVWQIREPIATLLLEGKTAGRHYAGPNWELADGSAVTAKAVAN
                     SPGATPNDIPWLKLEVKSQRGNGGFYGVTTVQRINTKGGVTQGPCDGAGTYQSVPYSA
                     DYVFLGNGS"
     gene            complement(5235..>6107)
                     /locus_tag="VGA15_24465"
     CDS             complement(5235..>6107)
                     /locus_tag="VGA15_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9590882.1"
                     /translation="MKQLRIGDITIDAVIEREGPWRRPQDFFPAYDEAIFKQHLPSME
                     PEVFDAALGMIVITYQTFVVRTPRYTILVDTCTGEDKGHPPPFDFPGKERWRNELFAL
                     GIAYDKVDYVFCTHLHIDHTGWNTTLRDGRWVPTFPNAKYIFHKKEYAVWQAEHARGA
                     NPPGTVFRDNCLPIVEAGQALLVDDDYALDDTITLTPTPGHSPCHCCVNIFSRGQRAV
                     VTGDMMHVIQCREPDWSARPDWDAKQSAVSRRKFLSSVAETDTLILPIHFPTPTAGLV
                     TADGDRFDYRFKRD"
BASE COUNT         1128 a         1962 c         1806 g         1211 t
ORIGIN      
        1 tgcgagaacg cggctttcat tctgcgaaga tgtcgccatg caagctttcg gcgcgcaccg
       61 gcaccggccg gatcgcctat gatttcgtgc atgccgcatt tggcgaagct gaaaaactgt
      121 ccccgtacaa cgccatcggc gtcgccgatt cgctcatcga tctcctgctg ctgcctcttc
      181 gccaggccgg aacgccgctc gactgcagtg gtccggtggc gatgtatgtg cgcgcccagg
      241 ccttcatccg cgaacacttg cgcgacccgg atctcagcat cgaccagatt tcggcggcat
      301 tgggctgcac caaacgctat ctgcacatgt tgttcagcga caggggcatg accgtcagcg
      361 attatatctg gcaggcaagg ttgcagaact gccgccagga gctggaggca cagggcggaa
      421 aaacaatcac cgatgttgcg ttttcgtggg gcttttcaag ctcgtcacac ttcagccgtg
      481 tcttccggaa atatttcgga attgttccgt cttcgatcca caaagcgcag cacggcagcc
      541 tgtccccgga tcttccctaa aacagaacgt ccaccagagg ccgcaagcag acgtggcctc
      601 atggctcgat atcgtcgcat gcgacgccgt gtggcggcgg acagacgatg gcttgttgag
      661 gtcgggcgcg gctttccgcc ttcttcccgg ctcagctgcg cagcccggga gcctcctgcc
      721 ccgtgcgcgc cacatactcc gtatagccgc cgccgtactg gtgaatgccc tcgggggtca
      781 gttccagcac ccgattggac agcgcggcca gaaagtgccg gtcatgcgag acgaacagca
      841 tggtgccctc gtactgcgac agcgccgtga tgagcatctg cttggtcgcc atgtccagat
      901 ggttggtggg ctcgtccagc accaggaagt ttggcgggtc gtagagcatc ttcgccatca
      961 ccagccgggc tttctcaccg cccgacaaca cccggcattt cttctcgaca tcgtcgccgg
     1021 agaacccgaa gcagccggcc agcgcgcgta gcgagccttg tcctgcttgc gggaacgaat
     1081 cctccagcga ctggaacacc gtgcgctcgc cgtccaacag atccatggcg tgctgcgcga
     1141 agtagcccat cttcacgctg ccacccagcg ccaccgtacc atcgtccggt tgcgtggagc
     1201 ccgccaccag cttcagcagc gtggacttgc cggcgccgtt gatacccatg acacaccagc
     1261 gctcccgccg gctcaccata aagtccagcc cctggtagat gcgcctgacg ccatagcctt
     1321 tgtgcacgtt cttcaggctc accacgtcct cacccgagcg cggcgccggc atgaagtcga
     1381 aagataccat ctggcggcgc ctgggcggct ccaccctttc gatcttctcc agcttcttca
     1441 cccggctctg cacctgggcc gcgtgcgagg cacgcgcctt gaagcgctcg atgaaattga
     1501 tttccttggc gagcatcgcc tgctggcgct cgaactgggc ctgctgctgc ttctcgctca
     1561 gcgcgcgctg ctgctcgtag aactcgtaat tgcccgaata ggtcgtcagc gcgccaccgt
     1621 cgatctcgat caccttgcca atgatgcgat tcatgaactc gcggtcgtgc gaagtcatca
     1681 gcagcgcgcc ctcataacct ttcaggaact gctcgagcca gatcaggctc tcgaggtcga
     1741 gatggttact cggctcgtcg agcagcatgg cgtccgggcg catgagaagg atgcgcgcca
     1801 gcgccacccg catcttccag ccacccgaca acgcgccgac gtcaccgtcc gtcatctcct
     1861 ggctgaaact caggccggcg aggacttcgc gcgcccgccc ctccagggcg tagccgtcca
     1921 gctcctcgaa gcgtgcctgc acctcgccat agcgctcgat gatatcgtcc ataccatccg
     1981 cccggtccgg atctcccatc gcagcttcga gctctttcag ctcgctcgcc acggcactga
     2041 cagggccggc gccgtccatc acctcggaca cagcacttcg gcccgacatc tcaccgacgt
     2101 cctggctgaa gtaaccgatg gtgacgcccc gatcgacggc cacctggccc tcgtcggggc
     2161 tctcctcgcc ggtgatcatc cggaacagcg tggtcttgcc tgctccgttg gggccgacga
     2221 ggccaacctt ctcgcccttc tggagcgctg cggaggcctc gatgaagaga atttggtggc
     2281 caatctgctt gctgacgtta tcgagacgaa tcatgtgcac cacgaggctt aagaaaagtc
     2341 gcggctgctc ttatgccatg tagcgggcct gcggaagggt gtttcggggt tggtcgctta
     2401 cgtgccgaaa ttggcgtgca attcggcatc attccgggcg agcggtcaaa ggccgcgagg
     2461 tccggccgta acgaccgagc agctaagcac gctgcacgtt ctttcccgca agcacggttc
     2521 cggccggaaa caaccgctga cggccggcgc ggatgccttt gtagttgtcg aggaactcga
     2581 acgcgccttc ccgcagctcc atgatcgcca cgtcggccgg ggcgcccaca ttgagcgtgc
     2641 cgcggtcatc gaaagacggg aacacgcgcg ccgcgttcac ggtggcacat gcaatgatct
     2701 ggctgagcgg catgccgaac atgatgaact tcgacatgac attcggcaaa tcgacgacgc
     2761 cggtcgtctt cgacgtcacg ctccaatcgg tggagaacgt atccggccat aggccttgcc
     2821 tggtcgcttt ttccaccgtg tcccagtcga agtgatcggc gacgccgttg ccgaagtcga
     2881 agagaacgcc gcggcggcgc gcagccgtca cctcgggaaa gagcacgccc ttgtcgtcga
     2941 gaatgccgtt cattcccggt gcgtacatgt gggtgacgat gtcgccgcgc ttgagtaatg
     3001 cgagaatggc acgcatcggc gaatagttct ggccgacatg aatcatgaca gggagattga
     3061 agggtgctgc cgcctcctgc gcgcgccgca acgcttcgag atcgttggcc ccggcgacgt
     3121 tattcgacag ccgcgccttg acgccaacga cgacatcgtg attgcgcgcg atggcccctt
     3181 gcgccaaggc cacgttggcg ctgttgaggt catgcaactc tccgccggga gagatgacgc
     3241 cggtccgggc gatgttcacc agggcgcggc cgatctgcgg cgcgccacgc gcgacggcgg
     3301 cgacctgatc gatggtatcg gctccgccgg agccgacatc gacccagcag gtcacgccat
     3361 cttgcaggca catcggcggc ccctccttgc ttcggccggc atgggtgtga atgtcgatca
     3421 ggcctggggc gacaatcttc ccgcgcgcat cgatcgtctc ggcggcgtcg gccgcgatgt
     3481 tggcctcgac cgctgcgatc cttccccgca tgatcgccac gtcgcgctcc gcgtcgagcc
     3541 ctacggaagg gtcgatgacc cgcccgcctt tgatcaccag atcgtaagtc gcagcgtacg
     3601 cctcggggac atttgcgagc agcgcagcac cggcggcgcc ggcgcaaata aactggcgtc
     3661 ggtccatcat ggtctatcct ccctgtgtgg ctggtctctt tgtcgcttgg ccctttgtta
     3721 tcaggttctt gttcgccagc gcgcgcgctc gcaatcggga aattcccaac gccggcggaa
     3781 tgcgttagag agattattcc acgcgctaaa ggacagagca acaacagagg caggagattg
     3841 caaccgtgga ccaaagcgtg aaacccgcac tgcttctcca atcctcaggc gatgccgcca
     3901 acacggaaag ccggatgcga cggcgggtgg tgctggcacg gcaaccgcat cgatgtcggt
     3961 tggccgatca gagcccgtca tcggcgcttc caacttggcc tccgtgcctg gtgggctgct
     4021 atgcactgcg tttcccactc gatgtcagga aacggatcgg cagaatgagc gtatcgatat
     4081 ccgggaccga cggatacagt tcagggtgtc gcggccgaat gaccgcgcgc gatagaaagg
     4141 tcccttatga accgttgccc aggaacacgt agtccgccga gtacggtacg ctttggtagg
     4201 tgcccgcgcc atcgcacggc ccctgcgtca cgccaccctt ggtgttgata cgctgcacgg
     4261 tcgttacgcc gtaaaatcct ccattaccgc gctggctctt aacttcgagc ttgagccacg
     4321 gaatgtcatt gggcgtcgcg ccaggcgaat tggccacggc cttggcagtt acagcacttc
     4381 cgtcggcgag ttcccaattt ggtcccgcgt agtgacgccc agctgtcttt ccttcaagca
     4441 gaagcgtggc gatcggctcg cggatttgcc aaaccagctt cttgtccggg ccgaccgtgc
     4501 attcgtagac ttgagcgccc tccgcatgga gcgtggtcac gatatactgg ccgggaacag
     4561 cgattgccga tggtacttgc gcattaacct ctcccgaaag cagcgtagcg agagcgacaa
     4621 gagagaacag cggcattttt agatgaggat gcatggattt tcttttcctt ggaatatggc
     4681 tcttttgggc gttccgcgta atgcgcggtc tcgagaaagg gctggttgac gatctcgccc
     4741 caatgagatg cggcccctat aaccgagtgt caggtccttc gcgtcatctg atcgcaggag
     4801 gttcgacctg ctcgctaccc gcatgcgcac caacgcggtc agatgtcaca agccgaagtc
     4861 atcgcggcca caggaagcaa gccggtgccg gcctactaca tcaccgccgt cgcaatctct
     4921 cgatcgtggt ggtcggcagc gcgcgaaata gcgtgcggcg cgccgcaatg gctcagccca
     4981 aagtgcgatt cgaaaagcgc cggccgcggc gcaaacctct ctttcgtcat gcccggactt
     5041 gttccgggca tccacgtctt gctgcggatg acgagaagaa acacgcctct cctcagtcct
     5101 tgcgaggagc gtaagcaacg aggcaatcca gtctttcctg cagctttctg gattgcttcg
     5161 cgggcctgtc atcgggcgcg cattcgcgcg acccgttggc tcgatgacgg caaatatttt
     5221 aatcgcccgc tcactcaatc ccgcttgaac cggtagtcga atcgatcccc atccgccgtg
     5281 accagtcctg ccgtcggtgt cgggaaatgg atcggcagaa tgagcgtatc ggtctccgcg
     5341 accgaggata agaatttccg acgcgacact gccgattgct ttgcatccca gtccggcctt
     5401 gccgaccagt caggctcccg gcactggatc acgtgcatca tgtcgccggt caccaccgcg
     5461 cgctgcccgc gcgagaaaat attgacgcag caatggcagg gcgagtgccc cggcgtcggc
     5521 gtcaacgtaa tggtatcatc gagagcatag tcgtcatcga ccagcagcgc ctggccggct
     5581 tccacgatag gtaagcagtt gtcgcggaat acggtgccgg gcgggttcgc tccgcgcgcg
     5641 tgctccgctt gccaaactgc atattccttt ttgtggaata tgtatttcgc attcgggaac
     5701 gtcggcaccc agcggccatc tcgtaaggtg gtgttccagc cggtatggtc gatatgaaga
     5761 tgcgtgcaga acacatagtc gactttgtcg taagcgatgc cgagcgcaaa cagctcgttc
     5821 cgccagcgct ccttgccggg aaaatcgaac ggcggcggat gacccttgtc ctcgccggtg
     5881 caggtgtcga ccaggatggt gtagcgcggc gtccgaacca caaaggtctg ataggtgatg
     5941 actatcatgc ccagcgccgc gtcgaacacc tccggctcca tcgacggcag atgctgcttg
     6001 aagatcgcct cgtcataggc cggaaagaag tcctgcggcc gccgccatgg gccttcgcgt
     6061 tcaatcaccg cgtcgatggt gatgtcgccg atcctgagct gcttcat
//
LOCUS       DASWGM010000900         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2486750,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000900 DASWGM010000000
VERSION     DASWGM010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486750"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VGA15_24470"
     CDS             complement(<1..584)
                     /locus_tag="VGA15_24470"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX9590883.1"
                     /translation="MTQPISVVIPVYDGVTQLDFTGPHQFLSRTPDLNVRVASLGGEP
                     VTADLTFSNLEDLTVIKSCDVLLVPGGGGCINAIENDRFIKEIVRLGATARYLTSVCT
                     GSLILGAAGLITGRRAACHWAWREFLPVFGAIVDEGRIVRDGNILSGGGVTAGIDFVL
                     ALIAELRGSRAAEMVQLGLEYAPEPPFNSGRPDTA"
     gene            701..1108
                     /locus_tag="VGA15_24475"
                     /pseudo
     CDS             701..1108
                     /locus_tag="VGA15_24475"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
     gene            1151..>1581
                     /locus_tag="VGA15_24480"
     CDS             1151..>1581
                     /locus_tag="VGA15_24480"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9590884.1"
                     /translation="MALIEEDHGFDIAKSVAQHLVVYHRRHGGQSQFAASVDLAPKNN
                     RISAAIGYARDHIAEALTLENLAEAVGMSRRQFSRAFRAQTGTTPARMVERLRADMAR
                     GRIEATTEPVERIAEAVGFGDAENMRRAFIRIYGQPPQSIRR"
BASE COUNT          304 a          498 c          484 g          295 t
ORIGIN      
        1 gccgtgtccg ggcgcccaga gttgaacggc ggttcgggcg catattccaa gccgagttgg
       61 accatctccg ccgcgcggga tccgcgcagt tccgcgataa gtgccagcac gaaatcgatg
      121 ccggccgtga cgcccccgcc ggacaagata ttgccatccc tcacgatgcg gccctcgtcg
      181 acgatggcgc cgaagaccgg aagaaattcg cgccatgccc agtggcaggc cgcacggcgt
      241 ccggtaataa gtccggcagc gcccaaaatg agcgatccgg tgcagaccga agtcaggtaa
      301 cgcgcggtcg ctccaagcct gacgatctct ttgatgaatc gatcgttctc gatggcgttg
      361 atgcagccgc cgcctccagg caccaaaagg acgtcacacg atttgatgac cgtcaggtct
      421 tccagatttg agaaggtgag gtccgccgtg accggttcgc cgcccaagga agccactcga
      481 acgttcagat cgggcgtgcg cgagagaaac tggtgcggac cggtgaagtc gagttgggtc
      541 acgccgtcgt acacgggtat caccacgctg attggctggg tcatcgctgt atccttctgc
      601 tggacaacct ctacagcccg ggttatatga ccggaaggtc aaacttccct atgatccggc
      661 catgcatacc atcggctttt atgtttctcc gtgctttcag atgcttgacc tcgcaggtcc
      721 ggccggcgcg ttcgaagcgg caaatgacga gctgggaagt ccggcctatc ggatccatat
      781 cctcgcggcc gatgcgggtg ccgtaacgaa cagccttggc atggctactg cagctgcgtc
      841 gctcgatgat gccgtgctcg atacgctcgt cgttatcggc ggaccaatcg agccacacct
      901 ttccgccggc gcgctaagcc gcatcgtgga cgcttcgaac cggagccggc gcgtggcgag
      961 cgtctgcacg ggcgcattca cgctcgcggc ggctgggctg ttggacggat gtcgagcgac
     1021 gacgcattgg cgatttgctg ctcgccttca gcgagactat ccatcgatcc gggtcgaggc
     1081 ggatcgcatc tactgccggg acggaaacat atggacttcg gcgggcatta ccgccggaat
     1141 cgatctctcc ttggctttga tcgaggagga ccatggtttc gacatcgcca agagcgtcgc
     1201 acagcacctc gtcgtctacc atcgtcgcca tggcgggcaa tcacaattcg cggcctctgt
     1261 cgatctggca ccgaagaaca atcgtatttc cgccgccatc ggctacgccc gcgatcacat
     1321 cgccgaagcg cttaccctcg aaaatttggc ggaagccgtc gggatgagcc gacgccagtt
     1381 cagtcgcgca ttccgggcgc agaccggcac cacgcccgcg cgtatggtgg aacggctccg
     1441 cgccgatatg gcccgaggtc gcatagaagc gaccaccgaa cctgtcgaac gcatcgccga
     1501 ggcggttggc ttcggcgacg cggagaacat gcggcgggcg ttcatccgca tctacggaca
     1561 gccgcctcaa tcgatcaggc g
//
LOCUS       DASWGM010000901         7919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_948260,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000901 DASWGM010000000
VERSION     DASWGM010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948260"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..855
                     /locus_tag="VGA15_24485"
     CDS             430..855
                     /locus_tag="VGA15_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9590885.1"
                     /translation="MAKILIVDDDNAVQMTIRLLLERAGHNVVVAGDGRKGLAVFEAG
                     DFDLLFLDIFMPGMDGLEIMRLVHQQRPLTPVIVISGNPIMEPGSGPDFLSMAGKLGT
                     VRGLQKPFKPAALLAAVTSCLESARENSKSPGRTAGNAA"
     gene            929..4438
                     /locus_tag="VGA15_24490"
     CDS             929..4438
                     /locus_tag="VGA15_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9590886.1"
                     /translation="MTLTTRLAVAMIALVAIAVTAVGWLSYHSLEQALLPRVLDRIET
                     HSRLVAADLQSHARGARADVAAFRSAAASNGLIRAHLNGGIDPVDGLSEKTWHDRIAE
                     RLLAELEAKPAYAQFRIIGIENGGRELVRVDRSGPNGAVRLVPDGELQRKGDRPYFRD
                     TISLPANEIYVSSLDLNQENGVIETPHVPTLRVGTRLLAPDGKPFGIVIINVDMRPAF
                     DRVRLSVRAGGQIYVVNKSGDYLAHPDATREFGSQLGKPTDWQSDFPELAPTLGATQG
                     VAHIVPDRTGQPGGSALAPAVLAGHEWVGVIETVPNAVIMAPAAAIQKTSVLVGLLAV
                     LSAAALAVLVARSLTRPIIQLRAAVEAAAGNESTAIPVEAGGETGVLARAFARVMGEV
                     NAKTAALEREIREHHRTETARDHYAARERLFSAAVESSHDAIVTKSLDGTITGWNPAA
                     ERLFGFTAAEAVGKGIHLIVPRDRLPEMHEILDRIGRGESIDLHETVRIRKDGSPVEV
                     SLSISPIKSASGAIIGASKTARDITESKRTQQALGQQIEERRRIFETSQDLILVSDPK
                     GVLVQVSPSAEAILGYLPEEMIGHSAIEFICRDDLDSTREEMRAARRGLHTRNFDCRY
                     VHKDGRIVTLSWMGTWSEPVRRHFFIGRDMTESRLAQETLRESEQLARGIVDTALDGF
                     VQIDETGTIADWNSQAEKIFGWSRSEALGKTLSELVIPEIHRAAHEAGLKHFLLTGEG
                     PILGRRLQIEARRRDGTEIKVELSITALRRRDGVMFNGFIRDLTDKIAAEERIRQTEK
                     MEAVGQLTGGIAHDFNNILTVITGTIEILADAVAKEPELAAIAKMIDEAASRGADLTG
                     HLLAFARKQPLQPRETDLNTLIIETVKLLRPTLGEQIEIESLFEDESCLATVDANQLA
                     TALLNLAINARDAMPDGGKLILETRSAVLDENYASLHSEVRPGRYALIAVSDTGTGIP
                     AGILDKVFDPFFTSKAPGKGTGLGLSMVYGFVKQSAGHIKIYSEEGHGTTIKIYLPPG
                     TGASLAPEAATAPAIPGGHETILVVEDDKLVRDSVLTQLRSLGYVTLEAANGAEALAV
                     IEAGHQFDLLFTDVIMPGAMNGRQLANEVEKSKPGLKVLFTSGYTENAIIHHGRLDTG
                     VLLLAKPYRKSDLAGMIRTALAD"
     gene            complement(4492..5127)
                     /locus_tag="VGA15_24495"
     CDS             complement(4492..5127)
                     /locus_tag="VGA15_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9590887.1"
                     /translation="MSENARSRGEIFVVDDDPAIREILSLVLSAGGYQVICFADGAAL
                     LAVARTRCPACILLDVHIPGKSGLDILKELHGEDYPAPIFMISGQGDIAMAVSAIKNG
                     ALDFIEKPFRGSEIVHRLDEAIEAYARRQAEDSASNVASMHFPGREPLTRREREVLEQ
                     FASGASNKEAGRELGISPRTIEDHRANIMKKLGARNAADLIRIVMTVSRNS"
     gene            5295..5615
                     /locus_tag="VGA15_24500"
     CDS             5295..5615
                     /locus_tag="VGA15_24500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590888.1"
                     /translation="MQNAEGGSVSARSDDRYDGFGSIVLDIVSLIERVQASTRLVESA
                     IESPLGHQEIAANVVNARAALNACNASLEVTLHLLLDSKRSKNGTDKAAEYDLRSARL
                     VGYA"
     gene            complement(5870..7546)
                     /locus_tag="VGA15_24505"
     CDS             complement(5870..7546)
                     /locus_tag="VGA15_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX9590889.1"
                     /translation="MFSRSNSVAQAALAQVAAISRSQAVIEFNPDGTIVTANENFLKT
                     LGYSLGEIQGKHHSMFVEPAMRESAAYREFWAKLNRGEYQAAEYKRIGKGGKEVWIQA
                     SYNPMLDRNGKVYRVIKFATDITAEKLGNMDDAGVITAIGRAQAVIAFNLDGTIITAN
                     ENFLKTLGYSLGEIQGKHHSMFVEPAMRDGAAYREFWASLNRGEYQAAEYKRIGKGGK
                     EVWILASYNPILDEKGKPFKVVKFATDVTEQKLRTADLAGQIAAIGKSQAVIEFNMDG
                     SILTANENFLNALGYSLREIQGKHHSMFVDAADRDSAAYREFWANLNAGRYQAGEFKR
                     IGKGGREVWIQASYNPILDLNGKPFKVVKYAADTTAQVIARKRAESVRGMMETVAAGA
                     EELNASVREISETMTKSRQTASEAVVRVDAADQQAQRLSAASEAMGGIVELIGNITGQ
                     INLLALNATIESARAGEAGRGFAVVASEVKNLANQAKQATDKIGQEIGNLNGISADVV
                     GALTAIKQSIQEVSEYVTSTAAAVEEQSTVTSEMSSSMQRAAAEAAGAGR"
     gene            complement(7739..>7919)
                     /locus_tag="VGA15_24510"
     CDS             complement(7739..>7919)
                     /locus_tag="VGA15_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX9590890.1"
                     /translation="IQGLNLKEFFLLLSSTGLAYRRCELVRVNGTEIDVQFLKGKNKK
                     KKPGVKAKQDAVVGG"
BASE COUNT         1621 a         2409 c         2305 g         1584 t
ORIGIN      
        1 ccttcgatgg cgggagcgtg cgacgaacgt gttggtatgc tcgcccaatc ggctgtcggt
       61 aaatccaagt ccggccgatt ttgagctcgt tgaaccgctt ctgcgtgact ttgaaccggg
      121 gcgggtttcg gagtgtggca attctccatc aacgatttga tcggtgatgt aaggtcggga
      181 ccgtaaaact acggagtgac atacgtgtcg gatcgcgatg ccttgcaaat ttcggcgcat
      241 cgaatttatc cgcatgacgt tctgatgcgt gtctgcccgc aatagtgcat atggaaagtt
      301 tctcagacat cgtattgaca aaatcagctg aacgcccacg ccgggaagtg cttgcgcgaa
      361 aatgcaaatt cagcgaaacg atgcaacctg cccttgacgt gagtccggat tcgatcggag
      421 aaatgggctg tggcgaaaat cctgatcgtg gacgacgaca atgcggtgca gatgacgatc
      481 cggctgctgc tggaacgggc tggccacaat gttgtcgtcg ccggcgacgg ccgcaaagga
      541 ctggcggtat tcgaagccgg cgatttcgat ctgctgtttc tcgatatctt catgccggga
      601 atggacgggc tggaaatcat gcggctggtt catcaacagc gcccgctgac cccggtcatc
      661 gtgatctccg gcaatcccat tatggagccg ggttccggcc cggattttct gagcatggcg
      721 ggcaagcttg gcacggtccg cggcctgcaa aaacctttca agccggccgc tctgttggcc
      781 gccgttacga gctgccttga aagcgcaaga gagaattcaa aatcgcctgg caggacagcc
      841 ggcaatgccg cataagccga tgtttcccac gcagggctca catctcggcg atctcgcgcg
      901 ctggcgaatt ccttacgatc tgaaggcgat gacgcttacc accagattgg ccgttgcgat
      961 gattgcattg gtcgccatcg cggtaaccgc ggtcggatgg ctgagctatc atagtctgga
     1021 acaggcgctg ctgccgcgtg ttctcgatcg catcgagacg cattcgcggc tggttgctgc
     1081 cgatctgcag tcccacgcgc gcggtgcgcg ggctgacgtc gccgcttttc ggtcggcggc
     1141 tgcatcgaat gggttgattc gcgcgcatct caatggcgga atcgatccgg tcgacggcct
     1201 ctccgaaaaa acatggcatg accggatcgc ggagcgcctg ttggccgaac tcgaagccaa
     1261 gccggcctac gcgcaatttc ggatcatcgg catcgaaaat ggcggacgtg agctggttcg
     1321 cgtcgatcgt tccgggccga acggtgcagt tcggctcgtt cccgatggcg agctgcaacg
     1381 aaagggcgac cggccttatt tcagggacac catcagcctg ccggcgaacg aaatatacgt
     1441 ttcgtcactt gatctcaatc aggagaatgg ggtgatcgaa accccgcacg tgcctacctt
     1501 gcgcgttggc acccgcctgc tggcacccga tggcaaaccg tttggaatcg tcatcatcaa
     1561 cgtcgacatg cgaccggcgt tcgaccgggt ccggttatcg gtacgcgcgg gcggacaaat
     1621 atatgtcgtc aacaagagcg gcgattatct cgctcacccc gacgccacgc gtgaattcgg
     1681 ctcgcaactt ggcaagccaa cggactggca gagtgacttt cccgagctgg cgccgacgct
     1741 cggcgcaaca caaggtgttg cgcatatcgt gcccgatcga accggacaac ccggtggctc
     1801 tgcgctcgcg cccgcggtgc tggccggtca cgaatgggtt ggagtcatcg aaaccgttcc
     1861 caacgcggtc atcatggcgc cagcggcggc catccagaaa acctcggtgc tggtcgggct
     1921 actggctgtg ctaagtgcgg ccgcgctggc ggtgctggtg gcgagatcgc tgacgcgccc
     1981 gatcattcaa ctgagggccg cggtcgaagc tgccgcaggg aatgagagca ccgccattcc
     2041 cgtcgaagcc ggcggcgaga ccggggtgct cgcgcgcgca ttcgcgcggg tgatgggcga
     2101 ggtgaatgcc aagaccgccg cgctcgaacg tgagatccgg gaacatcacc gcaccgaaac
     2161 cgcgcgagat cactacgcgg cgcgcgagcg tctgttcagc gccgccgtgg aatcgtccca
     2221 tgacgccatc gtcacgaaat ccctcgacgg taccatcacc ggctggaacc cggccgcgga
     2281 gcgcctgttc ggatttaccg cggcggaagc ggtcggcaaa ggcatccatc tcatcgttcc
     2341 ccgcgatcgc ctgcccgaga tgcacgagat cctggaccgg atcggccggg gtgaatcgat
     2401 cgacctccat gagacggtgc gcatacgcaa ggatggcagc ccggtcgagg tttcgctcag
     2461 catctctccg atcaagagcg cgtcgggcgc catcatcggc gcatccaaga ccgcccgtga
     2521 catcactgaa agcaaaagaa cccagcaggc gctcggccag cagatcgagg agcggcggcg
     2581 tatctttgaa acctcccagg acctgatcct ggtgtcggac cccaagggag ttctggttca
     2641 ggtgagccca agcgcagaag ccatcctggg atacttgccg gaggaaatga tcggtcacag
     2701 cgcgatcgaa ttcatctgcc gtgacgacct cgacagcacc cgcgaagaaa tgcgggcggc
     2761 gcggcgcgga ttgcacacgc ggaatttcga ctgccgctac gtccataaag acggacggat
     2821 cgtgaccctc tcatggatgg gcacatggtc ggagccggtc aggcgccact tcttcattgg
     2881 ccgcgacatg accgagagcc ggctggcgca ggaaacattg cgcgaaagcg aacaactggc
     2941 gcgcgggatc gtcgatacgg cgctcgatgg cttcgtccag atcgacgaaa ccgggaccat
     3001 cgcggactgg aattcccagg cggaaaagat attcgggtgg tcgcgcagtg aagcgcttgg
     3061 caaaaccctc agcgagctgg ttattccgga gattcaccgc gccgctcacg aggcaggtct
     3121 aaagcacttc ttgctcaccg gggaaggacc cattctcgga cgccgtctcc aaatcgaagc
     3181 tcggcgacgc gacggaaccg aaatcaaggt cgaattgagc atcaccgcgt tgcgacgccg
     3241 tgacggcgtt atgttcaacg gcttcatccg tgatctcacc gacaagatcg cggccgagga
     3301 gcgtatccgg cagaccgaaa agatggaagc cgtcggccag ctcaccggcg gaattgcgca
     3361 tgacttcaac aatatcctga cggtgatcac gggaaccatc gaaattctgg ccgacgccgt
     3421 cgcaaaggag ccggaactcg cggccatcgc gaagatgatc gatgaagccg cctcgcgagg
     3481 cgcggatctc accgggcatc ttcttgcgtt cgcccgcaag cagccgctgc agccgcggga
     3541 aaccgacctc aacaccctca tcatcgaaac ggtgaaattg ctgcgaccga ccctcggcga
     3601 gcagatcgag atcgaatcct tatttgaaga cgaatcatgt ctggccacgg tcgatgccaa
     3661 tcagcttgcg acagcgctgc tcaacctcgc catcaacgcc cgcgacgcga tgcccgacgg
     3721 cggcaagctt atcctcgaga ccaggtcggc cgttctcgac gaaaattacg cgagcctgca
     3781 cagtgaggtt cgacccggcc gctatgcgct gatcgccgtc agcgacaccg gaaccggcat
     3841 tcccgcaggg atcctcgaca aggtgttcga cccgttcttc acgtcgaagg cgcccggcaa
     3901 aggtaccggc ctcggcttga gcatggtcta cggtttcgtg aagcaatccg cagggcacat
     3961 caagatctac agcgaagagg gccacggcac gacgatcaag atctacctgc cgccgggtac
     4021 gggcgcctcg ctggctcccg aggccgctac cgcgccagcc attccaggcg gacacgagac
     4081 cattcttgtc gttgaagacg acaagctggt gcgagactct gtgctgacgc aattgcgctc
     4141 gctgggttac gtcacgctgg aagcggcgaa tggcgcggaa gcgctggctg tcatcgaggc
     4201 aggccatcag ttcgacctgc tcttcaccga cgtcatcatg ccgggcgcga tgaatggccg
     4261 ccagctggcc aacgaggtgg aaaagagcaa gcccgggcta aaagtgctgt ttacctcggg
     4321 ctacaccgag aatgcgatca tccatcacgg ccggctcgac accggggtct tgctgctcgc
     4381 caagccatat cgcaaatcgg atttggctgg gatgatccga acggcgctcg ccgactagac
     4441 tagaaatcaa gtccgggggg cagcgcccgg cgcaatacgc gccaacgcgg atcaggaatt
     4501 gcgcgaaacg gtcatcacaa ttcggatgag atccgctgcg ttccttgcac caagcttttt
     4561 catgatgttg gcgcgatgat cctcgatcgt gcgcggactg attccaagct cgcgcccggc
     4621 ttccttgttg gaagcgccgg acgcgaactg ctccaatacc tcgcgttcgc ggcgcgtcaa
     4681 aggttcgcgt ccgggaaaat gcatcgatgc gacattcgac gcggaatcct ccgcttgccg
     4741 acgtgcatat gcctcgatcg cctcatcgag ccgatgaacg atttcgctgc cccggaaagg
     4801 tttttcgatg aagtcgagcg caccgttctt gatcgcgctg accgccatgg cgatatcgcc
     4861 ctgcccggag atcatgaaga tcggcgcagg ataatcctcg ccgtgaagct ccttcaggat
     4921 atcgaggccg gatttacccg gaatatgcac atcaagcagg atgcaagcag ggcatcgggt
     4981 tcgcgcaacc gccaacagcg ccgcaccgtc tgcgaagcag atcacctgat agccacctgc
     5041 cgacaagacc agcgacagga tttcgcgaat ggcgggatcg tcgtcgacga caaaaatctc
     5101 gccgcgggaa cgtgcattct cggacatgtg ccatcgtcca tagagagtta gaaacagaca
     5161 gtgaaaatat tcgcacgcgc cgaaccgtcg taccacacct catatagaat cgggcctact
     5221 cgtatttgta cgggaccaag acttaacgca caagtagcag cggagacttt aaaacacagt
     5281 ccaggagagc acccatgcag aacgcggaag gcggcagcgt ttcagcccga tccgacgatc
     5341 ggtacgacgg ctttggttcc atcgtgttgg acattgtatc cttgatcgag cgcgtccagg
     5401 caagcacaag gctggtcgag tcagccatcg aatcgcctct tggtcatcag gaaatcgccg
     5461 ccaacgtcgt gaatgcgaga gccgcgttga acgcttgcaa cgcgagtctt gaggtaaccc
     5521 ttcaccttct gcttgattcc aaaagatcca aaaatggaac cgacaaggcc gccgaatacg
     5581 atctccggtc agcccgtttg gttggctacg cttgaacagc gccggataac ggtgcccgga
     5641 cgtacctgcc cccgtcttca cccgtccagg cctgatcggc cccgcagtct tgacggtcat
     5701 cgccggcacg atcggatttg ccgatttcaa accgccgcca cgtcgcggac gacgtcactg
     5761 atgccgacgg ccggatttga ttggataagc ccgagcatca ggcgagttcg gttcgcgcct
     5821 tgacgtttcc gggcctgcag gtgccacccc gccccggtct cgcgactgct tatcgtccgg
     5881 cgccggcagc ttctgcggcg gcgcgctgca tgctcgatga catctcgctc gttaccgtgc
     5941 tctgctcctc gaccgctgcg gcagtcgatg tcacatactc gctgacctcc tgaatcgact
     6001 gcttgatcgc ggtgagcgct ccgaccacgt cggcggaaat gccgttaagg tttccaattt
     6061 cctgcccgat cttgtcagtg gcctgcttgg cctggttggc gagattcttc acctcggagg
     6121 caacaactgc aaaccctctg ccggcttcgc ccgcgcgtgc ggattcaatg gtggcgttaa
     6181 gcgccagcag gttgatctgg ccagtaatat ttccgatcaa ctcaacgatg ccgcccatcg
     6241 cttcgctcgc tgcgctcaaa cgttgtgcct gttgatccgc cgcatcaacc ctgacaaccg
     6301 cctccgacgc cgtttgcctg gacttagtca tcgtctcgga gatttcgcgc accgaggcgt
     6361 tcaactcctc agcgcctgcc gcgaccgtct ccatcatgcc gcgcacgctt tcggccctct
     6421 tgcgggcaat cacctgagct gtggtgtctg ccgcgtattt gacgactttg aacggcttgc
     6481 cgttcagatc gaggatcggg ttgtaggatg cctgaatcca gacttcccga ccgcccttgc
     6541 cgatacgttt gaattctccc gcctggtacc gaccggcgtt cagatttgcc cagaattcgc
     6601 gataggccgc gctgtcgcga tcggcggcat cgacaaacat gctgtgatgc ttgccctgga
     6661 tctcacgaag cgaatagccc agcgcgttca ggaagttttc gttcgccgtg aggatggagc
     6721 catccatgtt gaattcgatc acggcttgcg atttaccgat cgcggcaatc tggcccgcca
     6781 gatcggcggt ccgcagcttc tgctcggtga cgtcggttgc gaacttcacg accttgaagg
     6841 gtttgccctt ttcatcaagg atcgggttat aggacgccag aatccagacc tccttgccgc
     6901 ccttgccgat gcgcttgtat tccgctgcct ggtattcacc acggttgagg cttgcccaga
     6961 attcacgata tgcggcgcca tcgcgcatcg ccggctcgac aaacatgctg tgatgcttgc
     7021 cctggatctc gccaagtgaa taaccgagcg ttttcaggaa attttcgttt gccgtgatga
     7081 tggtgccatc cagattgaac gcaatcaccg cttgcgcccg gccaatggct gtaatcactc
     7141 cggcatcgtc catgttgccg agtttctccg cggttatgtc ggttgcaaac ttgatgactc
     7201 gatagacctt gccattgcgg tcgagcatgg gattgtagga ggcctgaatc cagacctcct
     7261 tgccgccctt gccgatgcgc ttgtactcgg cggcctggta ttctccccgg ttgagcttcg
     7321 cccagaattc acgataggcg gcgctttcgc gcatcgccgg ctcgacaaac atgctgtgat
     7381 gcttgccctg gatctcgcca agcgaataac cgagcgtttt caggaaattt tcgtttgcgg
     7441 tgacaatcgt gccatccgga ttgaactcaa tcaccgcttg cgacctgctg attgctgcga
     7501 cctgcgccag cgccgcctgc gcgaccgaat tgcttcgtga aaacatctca aaattcccct
     7561 gtcccaagaa atgatcgtga ccgccactga ttcgaaacta attcggcagg attcgcgaaa
     7621 ccttaatttt ccagcaagta caactacggg ggtcgatccc cgaaaccaac catcgcccag
     7681 caacatgctt caatgctcat tcatgggcgc ccgggctgat gcagcccggc gggctgcgtc
     7741 agccgccgac gacagcgtct tgctttgcct tgacgcccgg cttctttttc ttgttctttc
     7801 ccttgagaaa ttgaacgtca atctcggtgc cgttgacgcg gacgagctcg caacgccgat
     7861 aggccagacc ggtcgacgac aacagcagga agaattcctt gagattgagg ccttgaatg
//
LOCUS       DASWGM010000902         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2194342,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000902 DASWGM010000000
VERSION     DASWGM010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194342"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VGA15_24515"
     CDS             <1..292
                     /locus_tag="VGA15_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX9590891.1"
                     /translation="YRETLKPWLIADLLILNDSLPRSLASCYGNLVRNLDQIGVAYGR
                     QGAAQRHARGVRNRLDHSHMDDIFQHGVHEFIQEFIADNGRLGEIITKQYLI"
     gene            483..1322
                     /locus_tag="VGA15_24520"
     CDS             483..1322
                     /locus_tag="VGA15_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX9590892.1"
                     /translation="MRLRIAHSTSYRYEPPATGVIQILRMTPGSHDGQYVAEWQIDVS
                     TDSRLDVHQDAFGNVTHVLTYGPIADLTITVGGLIETHDTGGVLRGTDERFPPSLFLR
                     STPLTEVNPAMATFSRELRSESEDDVLGFLHALMMQINEHMTFDEDPTNSGTSAVEAF
                     GLKRGVCQDYAHIFIACARSGGVPARFVAGHFLRADGMVNQQAGHAWAEAFVPDLGWV
                     GFDPANAICTTDAHARVAIGLDYLGAAPVRGTRYGGGMETLSVAVKVDQAGGRQGQSQ
                     SQS"
     gene            1601..>2235
                     /locus_tag="VGA15_24525"
     CDS             1601..>2235
                     /locus_tag="VGA15_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX9590893.1"
                     /translation="MTYCCGVLVRDGLVMIADTRTNAGLDNVSTFRKLHIFSKPGDRI
                     MAIASAGNLAISQSVLSTLTEGLEDPHTGDIETLLNAPTMFQAAQRIGRAIRAVHATE
                     GVALQSEDINFDVSFLFGGQIKGSRMRLFMVYPAGNFIECTTDTPYLQIGEHKYGKPV
                     LDRAMHYDVELYEALKTSLISMDSTMRSNLGVGLPIDVLVVRTDVCDADLN"
BASE COUNT          438 a          703 c          665 g          429 t
ORIGIN      
        1 ttatcgggaa accctcaagc catggctgat cgcagatctt ttgattctca acgactcgct
       61 gccgcgttcg ctggccagct gttacggcaa tctggtgcgc aatctcgacc agatcggcgt
      121 cgcctatggc cgccagggcg cagcccaacg ccacgcccgc ggtgtccgca atcgcctcga
      181 tcacagccac atggatgata ttttccagca cggcgtgcat gaattcatcc aggaattcat
      241 tgcggacaac ggcaggctcg gtgaaatcat cacaaaacag tatctgatct gataccgtca
      301 tggccgggct tgtcccggcc atccacgtct tgctttaaga agttaaaaga aagaaagacg
      361 tggatgcccg gggtcgcaga caagtctacg cagtctgcgc aaagcagaca tgctatgccc
      421 gggcatgacg atcttttttg atctaactat ctggcctgaa ctctagctct gtttggtctg
      481 tcatgcgcct gcgaattgcc cattccacca gctatcgcta cgaaccgccg gccaccggcg
      541 tgattcagat cctgcggatg acgcccggca gtcacgacgg gcaatatgtc gccgaatggc
      601 agatcgatgt ctcaacggac tcgcggctcg acgtgcatca ggatgcgttc ggcaatgtca
      661 cgcacgtgct gacctatggg ccgatcgccg atctcaccat cacggtcgga ggcctgatcg
      721 agacccacga taccggcggc gtgctgaggg gcaccgatga gcgctttccg ccaagcctgt
      781 tcctgcgctc gacgccgctg accgaagtca atccggcgat ggcgacattt tcgcgcgagt
      841 tgcgctcgga atcggaagac gatgtgctcg gctttctgca tgcgctgatg atgcagatca
      901 acgagcacat gacgttcgac gaagacccca ccaacagcgg gacctctgcg gtcgaagcct
      961 tcggactaaa acgcggggtg tgtcaggatt atgcgcatat tttcatagcc tgcgcgcgct
     1021 ccggcggcgt cccggcgcgc ttcgtcgccg ggcacttcct gcgcgccgac gggatggtca
     1081 atcagcaggc cggccacgcc tgggcggaag cgttcgtgcc ggatctcggc tgggtcgggt
     1141 tcgatccggc caacgccatc tgcaccaccg acgcgcatgc ccgcgtcgcc attggcctgg
     1201 actatctcgg tgcggcgccg gtgcgcggca cccgctatgg cggcggcatg gaaacgctgt
     1261 cggtcgcggt caaggtcgat caggccggcg ggcggcaggg tcagtcgcaa agccagtcgt
     1321 gaggtgaaaa cgccacgaaa cctcattccg ggacgcgccc acgggtccgc gcaaagcgcc
     1381 gccccgttga caggctccgc gcgaacctca gatgtgcaat tgcacatcgg ggaatcctga
     1441 ggttccgggt tcgctcgttc cactcgcgcc ccgggatgac gtttttaatg gttgtcatgt
     1501 cccacgcccc caaacctcgg cagcaatgcc caaatggggt tggagacccc cgcaaaattg
     1561 gctagtaatt ttgcgcgcgg tctgcgttcg gggatcggaa atgacctatt gctgcggagt
     1621 gctggtgcgg gacgggctgg tcatgatcgc ggacacaagg accaatgccg gcctcgacaa
     1681 cgtctcgacc tttcgcaaac tccacatctt cagcaagccg ggcgaccgca tcatggcgat
     1741 cgcgagcgcc ggcaatctgg cgatcagcca gtcagtgctg tcgaccctga ccgaaggact
     1801 tgaagacccg cataccggcg acatcgagac gctgctgaac gcgccgacca tgttccaggc
     1861 ggcgcagcgc atcggccggg ccatccgcgc cgtccacgcc accgagggcg tcgcgctgca
     1921 gtccgaggac atcaatttcg acgtgtcgtt tctgttcggc ggccagatca aggggtcgcg
     1981 gatgcggttg ttcatggtct acccggccgg caacttcatc gaatgcacca ccgacacgcc
     2041 ctacttacag atcggcgagc acaaatacgg caagccggtg ctcgaccgcg ccatgcatta
     2101 cgatgttgag ctctatgaag cgttgaaaac cagcctgatc tcgatggact cgacgatgcg
     2161 ctccaatctc ggcgtcggtc tgccgatcga cgtgctggtg gtgcgcaccg acgtctgcga
     2221 cgccgatctc aatca
//
LOCUS       DASWGM010000903         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_387423,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000903 DASWGM010000000
VERSION     DASWGM010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387423"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VGA15_24530"
     CDS             complement(<1..316)
                     /locus_tag="VGA15_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX9590894.1"
                     /translation="MSLESVRAFFAEEAPDIAVIESPISSATVALAAEAYGVEPGRIA
                     KTLSLRIGERVVLIVASGTSRMDNKKLKARFGGKPKMLGVDEVADITGHEVGGVCPFG
                     LKT"
     gene            complement(306..1052)
                     /locus_tag="VGA15_24535"
     CDS             complement(306..1052)
                     /locus_tag="VGA15_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX9590895.1"
                     /translation="MSIKAVVFDAYGTLYDIQSVAAVTEQAFPGYGEIITQVWRIKQL
                     EYTWLRSLMRRYEDFSVITRDSLSYTLRCLGLKHDPALFERIMEKYLHLDLYPDALAA
                     LAALQGRKLAILSNGSTGMLTTLVRNSGLDRVLDATISIDSQRIFKPSPDAYALIESR
                     LGVPPAEVLFVSSNPWDACGAKAFGLNVAWIERVTPEAMALACVKSDVVAPLTMFKAI
                     RTQMDELGLEPDYRIHALSELPDLVAAHEP"
     gene            complement(1079..1963)
                     /locus_tag="VGA15_24540"
     CDS             complement(1079..1963)
                     /locus_tag="VGA15_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9590896.1"
                     /translation="MSKLKLPNVDDAVAIDIHTHAEEPCGMHADDGYDDFQAQMAEYF
                     KSPNKHPPTVPETAAYYRAKKIVAVIFPVDAERETGFRRYSNYEMLEVAADNSDVLIP
                     FVSIDPHKGKLGVREARKLIEEYGVKGFKFHPTMQGFYPNDRMAYPLYQAINDGGAIA
                     LFHTGQTGVGSGMPGGMGMRLKYSNPMYMDDVAADFPDLKIILAHPSFPWQEEALSVA
                     THKPNVYIDLSGWSPKYFPPILVRYINSILQDKMLFGSDWPVITPDRWLSDFAKLEIR
                     DEIRPKILKANARKLLNI"
     gene            2090..2566
                     /locus_tag="VGA15_24545"
     CDS             2090..2566
                     /locus_tag="VGA15_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9590897.1"
                     /translation="MAQAETFETDFWKDANLRKVWDDIVPGEPRKTIPYTLTRQAIEL
                     YCKSVGEDHPVYFDEAYAKTTRHGGLIAPPSIHILLMFACTPADDWMRSPGTVNAGQS
                     WSYHIPARPGDVITLQARALDKFIKRERLFVVHDNVFFNQHGEVICSGRGQTIRPM"
BASE COUNT          518 a          760 c          800 g          506 t
ORIGIN      
        1 gcgttttcaa tccgaacggg cagacgccgc cgacttcatg gccggtgatg tcggcgacct
       61 cgtcgacgcc cagcatcttc ggcttgccgc cgaagcgcgc cttgagcttc ttgttgtcca
      121 tccgcgaggt gccgctggcg acgatcaaca ccacgcgctc gccgatccgc aagctcagcg
      181 ttttggcgat ccgtccgggc tcgacgccat aggcttcggc ggccagcgcc accgtggcgg
      241 aactgatcgg cgattcgatc acggcgatgt cgggcgcctc ttcggcaaaa aacgcgcgaa
      301 cggattcaag gctcatgcgc ggcgaccaga tcgggcagtt ctgacagtgc gtggatgcga
      361 tagtcgggct cgagcccgag ctcgtccatc tgtgtccgga tcgccttgaa catcgtgagc
      421 ggggcgacga catcgctttt gacacaggcc aatgccatcg cttccggcgt cacccgctcg
      481 atccaggcga cattcagccc gaacgccttg gcgccgcagg cgtcccaagg gttcgacgat
      541 acgaacagca cctccgcagg aggcacaccg agcctggatt cgatcaaggc gtaggcatcc
      601 gggctgggtt tgaagattct ttgggagtcg atgctgatgg tggcgtcgag cacgcggtcg
      661 agaccgctgt tgcggaccag cgtggtgagc atgccggtgc tgccgttgga aagtatcgca
      721 agctttcggc cttgcagcgc cgcaagcgct gcgagcgcat ccggataaag gtcgaggtgc
      781 agatattttt ccatgatccg ctcgaacagg gccgggtcgt gtttcaggcc aaggcatcgc
      841 agcgtgtacg acagcgagtc gcgcgtaatg accgagaaat cctcgtagcg ccgcatcagc
      901 gagcgcagcc aggtgtattc gagctgcttg atacgccaaa cctgggtgat gatctcgccg
      961 tagccgggaa acgcctgttc ggtgacggcg gcgaccgact gaatgtcgta cagcgtgcca
     1021 taggcgtcga acacaacggc tttgatgctc acgggaatac tcgtgcaatc ggtcggcgct
     1081 agatgttcaa gagcttgcgc gcgttggcct tcaggatctt cggcctgatc tcgtcgcgga
     1141 tttcgagttt tgcaaaatcc gaaagccatc ggtcgggcgt gatcaccggc cagtccgagc
     1201 cgaacagcat cttgtcctgc aggatcgagt tgatgtagcg caccaggatc ggcggaaaat
     1261 atttcggcga ccagccggac aggtcgatat agacgttcgg cttgtgggtc gcgaccgaca
     1321 gcgcttcttc ctgccaggga aacgacgggt gggcgagaat gatcttgagg tcggggaaat
     1381 cggctgcgac atcgtccatg tacatcgggt tggaatattt cagccgcatc cccatcccgc
     1441 ccggcatgcc ggagccgacc ccggtctggc cggtgtggaa cagcgcgatc gcgccgccat
     1501 cgttgatggc ttggtaaagc ggataggcca tgcgatcgtt ggggtagaag ccctgcatgg
     1561 tcggatggaa tttgaaaccc ttgacgccgt attcctcgat cagcttgcgc gcttcgcgaa
     1621 cgccgagctt gcccttgtgc ggatcgatgc tgacgaacgg gatcaacaca tcggaattgt
     1681 cagccgcgac ttccagcatc tcgtaattgc tgtagcggcg gaagccggtc tcgcgctcgg
     1741 catcgaccgg gaagatcacc gccacgatct tcttggcacg gtaataggcc gcggtttccg
     1801 gcacggtggg cggatgcttg ttcggcgact tgaaatattc agccatctgc gcctggaagt
     1861 cgtcgtagcc gtcgtcggca tgcatgccgc acggctcttc cgcatgggtg tggatatcga
     1921 tcgccacggc gtcatcaaca ttgggcagct tcagtttgga catcgtggtt ttcccggcag
     1981 cggctcgatt gcacagtgtg gcaaattgat tataccatat aacaaaattg gcaaggcacg
     2041 gaaacagcaa gacacggaaa cagcaagaca cggaaacagg gaggatagca tggcgcaggc
     2101 ggaaaccttc gagaccgatt tctggaaaga tgccaatctg cgaaaggtct gggacgatat
     2161 cgtccctggc gaaccgcgca agaccattcc ctacacgctg accaggcaag cgatcgaact
     2221 ctactgcaaa tccgtcggcg aagatcatcc ggtctatttc gacgaggcct acgccaaaac
     2281 cacccgtcat ggcggcctga tcgccccgcc gtcgatccac atcctgctga tgtttgcctg
     2341 cacgccggct gacgactgga tgcgcagtcc cggcacggtc aacgccgggc aatcctggag
     2401 ctaccatatt ccggcgcggc ccggcgacgt catcacgctg caagcccgcg cgctcgacaa
     2461 gttcatcaag cgcgagcggc tgttcgtggt gcacgacaat gtcttcttca accagcatgg
     2521 cgaagtgatc tgttccggcc gcggccagac catccggccg atgtaagggg acgggatcat
     2581 gggc
//
LOCUS       DASWGM010000904         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1800763,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000904 DASWGM010000000
VERSION     DASWGM010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800763"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /gene="ilvN"
                     /locus_tag="VGA15_24550"
     CDS             <1..276
                     /gene="ilvN"
                     /locus_tag="VGA15_24550"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX9590898.1"
                     /translation="RVVDMTLTGRSIERELAMVKLRGTGEHRVEALRLAEAFRARVID
                     ATTESFVFEITGNSAKINQFIDLMRPLGLVEVSRTGVAAIGRGAEGM"
     gene            280..975
                     /locus_tag="VGA15_24555"
     CDS             280..975
                     /locus_tag="VGA15_24555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9590899.1"
                     /translation="MLARDWYYNERRQIGLDTAVASIYDTDDDSDLRARAALTMLGVQ
                     RGWRVADIGCGNGVLACEAALIGAEVDAIDIAPAMLRLAEIQARDRKVAIRTQSAGLL
                     SFAYQPDTYDLIVSEFTLHHLPDFWKAVALSRIYGALKPGANFYLRDIVYVSMPDGSE
                     RNVGAWADFNIKNHDFARDSVMTHMRDEYSTFGWVMERMLTDVGFTLVSADYHAPLHG
                     TYLLRKPKSGEQS"
     gene            986..1906
                     /locus_tag="VGA15_24560"
     CDS             986..1906
                     /locus_tag="VGA15_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX9590900.1"
                     /translation="MKPFDVFLAVLVAVIWGLAFVASRLALDELSPALMTAMRFAIAA
                     APCLFVRRPKVPWTVLIAISGTLFLGQFLAQSWAIAHGVPVGLASVIVQSQALFTVAF
                     AAAAFREIPARRQIAGIGVATIGLLMIGGTVGLDFSIGAFAVLMVSPVSFAIGNLLLR
                     QARDVAMFDLFAWLCLVPPLPLLALAFAVDGPRATWHSLSDMSPTGWVSILFIGAVST
                     CIAYWLWGRLLRDYTAAQVVPFALLVPFVGAGASSVVFGETFGPLRLTGMVTVVGGIA
                     IMLLSKRPQVVPKQVVPKQVLPKQVLPEIA"
     gene            1914..2516
                     /locus_tag="VGA15_24565"
     CDS             1914..2516
                     /locus_tag="VGA15_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX9590901.1"
                     /translation="MSQSLFVAFVVFATVMFFTPGPNNIMLLSSGLTYGFRRTLPHVA
                     GITIGFAFMIGAVGLGLGTIFISYPVLQTILKYAGVAYLVYLAAAIAMSGPVKPDQDN
                     RRGPMTFWGAAMFQWINVKGWVMVIGTITAYAGIARFPWNITIQVMLSLLLGVVSCSV
                     WALFGSALRPLLTSPRAIRAFNIVMAVLLLASLYPVFMDA"
     gene            2689..>2809
                     /locus_tag="VGA15_24570"
     CDS             2689..>2809
                     /locus_tag="VGA15_24570"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX9590902.1"
                     /translation="MRVYYDRDADLNLIKGKKVCIVGYGSQGHAHALNLKDSGV"
BASE COUNT          453 a          877 c          905 g          574 t
ORIGIN      
        1 cgcgtcgtcg acatgacgct gaccggccgc tcgatcgagc gcgagctggc gatggtgaaa
       61 ctgcgcggca ccggcgagca ccgcgtcgag gcgctgcgtc tggcggaagc gtttcgcgcg
      121 cgcgtgatcg atgccaccac cgagagcttc gtgttcgaaa tcacgggcaa ctccgccaag
      181 atcaaccagt tcatcgacct gatgcgacca cttggcctgg tcgaggtctc gcgcactggc
      241 gtcgccgcga tcggccgcgg cgcagaaggg atgtgaacca tgctggcgcg tgattggtac
      301 tacaacgagc ggcggcagat cggtctcgat accgcggttg cctcgatcta cgacacggac
      361 gatgacagcg acctccgcgc ccgcgcggcg ttgaccatgc tcggcgtcca gcgcggctgg
      421 cgggtggccg acatcggttg cggcaacggc gtgctggcct gcgaggcggc gctgatcggc
      481 gccgaggtcg acgccatcga tatcgcgcca gccatgctgc ggctggccga aatccaggcg
      541 cgcgaccgca aggtcgcgat ccggacccag tcggcggggt tgctcagctt cgcctatcag
      601 cctgacacgt atgacctgat cgtgagcgaa ttcacgctgc atcatctgcc ggatttctgg
      661 aaggcggtgg cgctgtcgcg gatctatggc gcgttgaagc ccggcgcgaa tttttacctg
      721 cgcgacatcg tttacgtcag catgccggat ggctccgagc gtaacgtcgg ggcgtgggcg
      781 gatttcaaca tcaagaacca cgattttgcg cgcgacagtg tgatgaccca tatgcgcgac
      841 gaatattcga cttttggctg ggtgatggag cgtatgctga ccgacgtcgg cttcacgctg
      901 gtctcggccg attatcacgc gccgctgcac ggcacctatc tgttgcggaa accgaagtcc
      961 ggcgagcaaa gctagagcga cgtcgatgaa gcctttcgac gtctttctgg ccgtcctggt
     1021 ggcggtgatc tggggtcttg cgttcgtggc cagcaggtta gcgctcgacg aactctcgcc
     1081 ggcgctgatg acggcgatgc gctttgccat cgccgccgca ccatgcctgt tcgtgcgtcg
     1141 cccgaaggtt ccctggaccg tcctgatcgc gatcagcggc acgctgtttc tcggacagtt
     1201 tctggcgcag tcgtgggcga tcgcgcacgg cgttccggtc gggcttgcca gcgtcatcgt
     1261 tcagagccag gcattattca cggtggcgtt cgcagcagcg gcgtttcgcg aaattccagc
     1321 gcgccggcaa attgccggga tcggtgtcgc gacgatcggc ctgttgatga tcggcggcac
     1381 cgtcggtctc gacttcagca tcggcgcgtt cgccgtgctg atggtctcac cggtgagttt
     1441 tgcgatcggc aatctgctgt tgcggcaggc gagggacgtc gcgatgttcg atctgttcgc
     1501 atggctctgc ctggtgccgc cgctgccgct gttggcgctg gcgttcgccg tcgacggacc
     1561 gcgggcgacc tggcactcgc tgtccgacat gtcgccgacc ggctgggtca gcatcctctt
     1621 catcggtgcg gtctcgacct gcatcgccta ttggctgtgg ggacgcttgc tgcgggacta
     1681 caccgcggca caggtggtgc cgttcgcgtt gctggtgccg tttgtcggcg ccggggcatc
     1741 gagcgtcgta ttcggcgaaa cctttggacc gttgcgcctg accgggatgg tcaccgtggt
     1801 cggcggcatc gcgatcatgc tgctgtcgaa gcggccgcaa gtcgtgccga agcaagtcgt
     1861 gccgaagcaa gtcctgccga agcaagtcct gccagagatc gcctgagtgc aacatgtcgc
     1921 aatcgctttt cgtcgctttc gtcgtattcg ccaccgtgat gttcttcacg ccgggaccga
     1981 acaacatcat gctgctgtcg tccgggctga cctacgggtt tcgccgcacg ctgccgcatg
     2041 tcgcgggcat cacgatcggt ttcgccttca tgattggggc ggtgggcctt ggcctcggga
     2101 ccatcttcat cagctatccc gtgctgcaga ccatcctcaa atacgccggc gttgcctacc
     2161 tggtctatct cgccgccgcg atcgccatgt ccgggccggt taagcctgat caggacaacc
     2221 ggcgcggtcc gatgacgttc tggggcgcgg cgatgttcca gtggatcaac gtcaagggct
     2281 gggtgatggt gatcggcacc atcaccgcct atgccggcat tgcgcgattt ccctggaaca
     2341 tcacgatcca ggtcatgctc agcctgttgc tgggcgtggt atcctgctcg gtctgggcgc
     2401 tgttcggcag cgcgctgcgg cccctgctca catccccgcg tgcgatacgg gcgttcaaca
     2461 tcgtcatggc cgtattgctg ctggcctcgc tctatccggt attcatggac gcatgatgtg
     2521 gcactgcgtc atgcagaaac gggtttcccc tcaggttaaa aatggactag acaacgccgg
     2581 aaatcgccag ccgaaccagc gcgccggacc ggcatccgca ccgaccggcc tgacttcaca
     2641 acatacgagg atcccggcac tccgcccggc cagccaagag gaacgaccat gcgtgtttat
     2701 tacgatcgcg acgccgacct gaacctcatc aagggcaaga aggtctgcat cgtcggctat
     2761 ggcagccagg gccatgccca cgcgctgaac ctgaaggatt ccggcgtca
//
LOCUS       DASWGM010000905         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1801138,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000905 DASWGM010000000
VERSION     DASWGM010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801138"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..959
                     /locus_tag="VGA15_24575"
     CDS             195..959
                     /locus_tag="VGA15_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX9590903.1"
                     /translation="MDWISFLLIPVALYLIVSLRVLKQYERGVVFLLGKFAGVRGPGL
                     TLIFVPIQQMVRVSLRTVTMQIPSQKIITKDNVSIDIAAVAYYHITDPEKAVIAIENV
                     YNAINQISQTTVRNVVGRFSLDQLLSDTASINDQIKNVIDLHTEPWGTQVTAVEIKDI
                     TLPDNMQRAMAKEAEAERERRAKIVAAEGEYQAAVKLGQAADIITQHPVALQLRTLQT
                     MAEISVEKNSTIIFPAQFMTTVQEAIALLSKDLASK"
     gene            complement(1066..>1552)
                     /locus_tag="VGA15_24580"
     CDS             complement(1066..>1552)
                     /locus_tag="VGA15_24580"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9590904.1"
                     /translation="RRLHRGSPDTSDPGATGRFVMLTRRDFAGIASCALCAATGFIAT
                     DALAQGMPPATTPGVTRKILSQTGGPTPGYVTIIVEAEIETGVTVARHMHPGIESAFI
                     IEGGLELPIEGQPTRALKPGDGFVAPPNTPHAGGKIGDKKTKIASTYVVEKGKPLASP
                     A"
BASE COUNT          315 a          492 c          440 g          305 t
ORIGIN      
        1 ctaagcgagc ctgcggcctt ggcgacaaaa tcgtctccaa atgactgggc gaatgacgat
       61 gtcggccgtc aatttgccct ggttttattg ggtaattgtc acccatcggc acaaatgcat
      121 gcgtttcgcg acagaaacta gtctagctca ggcgcggcgc ggccgttgcc accgcaacaa
      181 gggatgtggg caccatggac tggatctcct ttcttctcat accggtggcc ctctatctca
      241 tcgtctcgct tcgtgtgcta aagcaatacg agcgcggcgt agtgtttctg ctcggcaaat
      301 ttgccggcgt gcgcgggccg gggctgacgc tgatcttcgt tccgatccag cagatggtgc
      361 gcgtgtcgct gcgcaccgtc accatgcaga tcccctcgca aaagatcatc accaaggaca
      421 acgtctcgat cgacatcgcg gcggtcgctt actatcacat caccgatccg gaaaaggccg
      481 tgatcgcgat cgagaacgtc tacaacgcga tcaaccagat cagccagacc accgtgcgca
      541 acgtggtggg acgcttcagc ctcgatcagt tgctgtcgga taccgcgagc atcaacgacc
      601 agatcaagaa cgtcattgac ctgcacaccg agccgtgggg cacccaggta acggcggtcg
      661 agatcaagga catcacgctg cctgacaaca tgcagcgtgc gatggccaag gaagccgagg
      721 ccgagcgcga gcgtcgcgcc aagatcgttg ccgccgaagg tgaataccag gcggcggtca
      781 agctcggcca ggcggccgac atcatcacgc aacatcccgt ggcgcttcag ctccgcaccc
      841 tgcaaaccat ggcggaaatc tcggtcgaga agaattccac catcatcttc ccggcccagt
      901 tcatgacgac cgtgcaggaa gccatcgcgc tattgtcaaa agatttggcg tcaaaatagc
      961 ccgacgccgc cgtcacggta cgatcggcgg gttgatgccc ggtttgtggc ccggcccgtc
     1021 gcaagagtcc gattgcagca gtcaaaagtc gaaaacttga tcggatcagg ccggagatgc
     1081 gagcggcttg cccttctcca cgacgtaggt gctcgcgatc ttggtctttt tgtcaccgat
     1141 cttgccgccc gcatggggcg tgttgggcgg cgccacgaaa ccgtcacccg gcttcaacgc
     1201 ccgggtcggc tgtccttcga tcggcagctc cagtccacct tcgataataa aggcggattc
     1261 gattcccggg tgcatatgcc gcgcgaccgt tacgcctgtc tctatttccg cttccacgat
     1321 gatcgtcaca tatcccggcg tcggaccgcc agtctgcgac agaatttttc gtgtgacccc
     1381 cggcgtcgtc gctggcggca tgccttgcgc caatgcatcg gtcgcaatga accccgttgc
     1441 cgcgcagagc gcgcaggacg caatacctgc aaagtctcgc cgtgtcagca tgacgaacct
     1501 ccctgtcgcg cccggatcac tggtgtccgg acttccgcga tgcaaccggc gc
//
LOCUS       DASWGM010000906         9947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_949567,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000906 DASWGM010000000
VERSION     DASWGM010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949567"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /gene="efp"
                     /locus_tag="VGA15_24585"
     CDS             <1..520
                     /gene="efp"
                     /locus_tag="VGA15_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX9590905.1"
                     /translation="DGKLYVVLTAENIHPGKGTPVSQIEMRRISDGVKVSERYKTTDQ
                     VEKATIEDQNFNYLYKDADGFHFMNNDNYDQVQVPDDVIGSAAPYLQENMTVKLSLHG
                     VIPVAIQMPQRATLEVVETEPVTKGQTASSSYKPAILSNGVRTLVPPHIGTGTRIVIM
                     TEDGSYVERAKD"
     gene            547..2217
                     /locus_tag="VGA15_24590"
     CDS             547..2217
                     /locus_tag="VGA15_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEX9590906.1"
                     /translation="MPLGGDALGTEAVRLASRLLASVVVLLAGTVVPDADEFRTAAIS
                     AVRVEWRAALDQFRSEINTRPAVASVFTFSGQRRLPAFDPRTTPALVQLNAATSPIFT
                     GIGRSPVPVLLPFETAAFLDARLSAAPETLSLSRYQADFRSADLFDAGPAGYDAMFAL
                     EPGAGDGMPQRTFARPVEVQITGSILIYDISDPLGDKGEPVKALSAQYPDLRRFIREG
                     YVRYAFTRFGVPYVVSIQCLDSAPRAGRLACREAYPVAERFLRALRIAGGQPARPRMD
                     IAAVIAERPEARSADFTYLPSGDIIPNSGARKQSGHADFTAYSQIRFPLEKAPAFARS
                     QSFGNRRSYDRPLMPDEPAGGNTGYPWRDNFCEARSFSVGQCASGFGHQGQDIRPACS
                     PGNESADRCDPYRQAVVAVRDGVLIRSPQQQAATLQINTRNEHIRFRYMHMNPRSMDA
                     DGILNGRHVDEGEKLGVVSNYLDHPNGTSRHLHFDVQVFTRDGWLWVNPYVTLVSAYE
                     RLIHGRGREIGPEVAALPAVTHASAAPAVAHPSPGDAIDSGTQEGAEN"
     gene            2307..3542
                     /locus_tag="VGA15_24595"
     CDS             2307..3542
                     /locus_tag="VGA15_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX9590907.1"
                     /translation="MKQPDSRKGLTRRTLLQSGLGAGVLLARPMAAFATAPPGFDQWR
                     DGFRARALAKGISDATYARVMGRIEPDMSVFKQMQKQPEFNEQIWQYINRRVSDWRII
                     AGKEALKKNEALFARIEQDFGVERGTLLALWGVESAFGDPLVQQNHMRPVFPSLAALA
                     WNEPRRRAYWETELINALKIVDRGWSTPEEMRGSWAGAMGHTQWMPEVWLNVGMDYDK
                     DGRVSPFGKPDDALGSSARFLVTRGKYRRGEHWGYEVRAPGAASSGSRSYAAWSSAGV
                     SRADGQPFAQPNASAQMWVPTQGGPAFLLGPNFYAVRSYNPSMNYALAICHLGDRILG
                     APPFLQPFPGSERALTLAEVQEMQVRLTKAGFDTGGTDGRVGNDTMQAIRDYQAKMGL
                     LPADGYGGLKVLARLRQGS"
     gene            complement(3645..3920)
                     /locus_tag="VGA15_24600"
     CDS             complement(3645..3920)
                     /locus_tag="VGA15_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX9590908.1"
                     /translation="MARTVRKSGVKEVPLSEVKDDLSRYLREAETQEIIITRHGRPAG
                     ILIGFGSEEDWFEYRLENDPRFLRRIEQARDSLQAGRGVRLEDVETE"
     gene            complement(4076..5917)
                     /gene="recJ"
                     /locus_tag="VGA15_24605"
     CDS             complement(4076..5917)
                     /gene="recJ"
                     /locus_tag="VGA15_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX9590909.1"
                     /translation="MTLPASALPIEAPPAFLGVSNSATGKLWRDRLDARGAARALAIT
                     QRYQLPEMLARVLAGRDVEIDAVEDFLDPTIRKLMPDPDTVTQMETAAKRIADAAIRK
                     EKVAIFGDYDVDGATSAALLTWHLRHCGLDPLIHIPDRIFEGYGPNVEAVRMLAGKGA
                     TLLVTVDCGTTSFEPLAEARRLGMAVVVIDHHQSGDELPDVDALVNPNRPDDLSGLGH
                     LAAVGLVLVTLVAVNRELRARGFWTGEMPEPDLLGMLHHVALGTVADVAPLIGLNRAF
                     VAKGLIAMRRRDHVGHTALMDVSRLNGPPEAWHLGFMLGPRINAGGRIGRADLGVRLL
                     LEGDISEAAAIAAELDRLNTERRVIEQMAEAQAEAEALASLGLEDKGSVIVTASEGWH
                     PGVVGLVASRLKEKFSRPAFAIALEPGGIGTGSGRSISGVDLGKAVRQAVKDGLLMKG
                     GGHAMAAGVTLRKERLAEFRAYMESALAADVANSRHENELFIDGAVSARAVTPEFAAT
                     LNRAGPFGSGNPEPVIALPSHQLVYADEVGQAHLRVRFKSGDGAIVNGIAFRSIGQKL
                     GSALTQHRGQPLHVAGSLSVDRWQGTERVQFRVLDIAVPDQGPAMIR"
     gene            complement(7118..7390)
                     /locus_tag="VGA15_24610"
     CDS             complement(7118..7390)
                     /locus_tag="VGA15_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HEX9590910.1"
                     /translation="MRIPALAILTIATVSAAPSARAQTYDPAYPVCLQIYQGWNDYYF
                     ECAYTSLAQCNASASGRAALCMVNPYYAGRKTVPPGRRDRRHRRVD"
     gene            complement(7567..8286)
                     /locus_tag="VGA15_24615"
     CDS             complement(7567..8286)
                     /locus_tag="VGA15_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX9590911.1"
                     /translation="MSDISAVKALVFDVFGTVVDWRTSLIADFTKWADKRGVKADWTA
                     LVDGWRAVYAASMDEVRKHPERGYVILDRLHRQSLEKLVAQLSIEGLNDADLHHLTMG
                     WHRLNPWPDSVAGLTRLKTKYIIAPLSNGNFALLTNMAKFAGLPWDLVLSAELFEHYK
                     PDPETYLGAAKLLGLPPEQVMMVAAHNHDLKAAQKLGLKTAFVARPTEYGPLQKYDFE
                     AKGDWDIVAKDFGGIADRMGC"
     gene            8889..9476
                     /locus_tag="VGA15_24620"
     CDS             8889..9476
                     /locus_tag="VGA15_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590912.1"
                     /translation="MSKWLKQFLFGIWGLLLIPLITPILEKWLEENVFSDPNGMAATV
                     FPNVMATTVFSNLLALGQQRWFKFALVFLTGIVIGVSLEWLNRKSDEKKASELRSLGS
                     KFRSLSDNIKVRTASSGWPDNVRDLKPAILSAFISAKKFDLWVPNEHVYQLPDASFLC
                     EYFRCVGRLLEDGHFDEANREALSWKPFLDKAKLS"
BASE COUNT         1913 a         3140 c         2974 g         1920 t
ORIGIN      
        1 agacggcaag ctttacgtgg ttctgaccgc cgagaacatc caccccggca agggaacacc
       61 ggtcagccag atcgaaatgc gccgaatcag cgacggggta aaggtttccg agcgctacaa
      121 gaccaccgac caggtggaaa aagcgaccat cgaggaccag aatttcaact acctttataa
      181 ggacgccgac ggcttccact tcatgaacaa cgacaattac gatcaagttc aggtgcccga
      241 cgacgtcatc ggcagcgccg cgccctatct gcaggaaaac atgaccgtga agctttcgct
      301 gcacggcgtg attccggtcg caatacagat gccgcagcgc gcgacgctgg aggtggtgga
      361 aaccgaaccg gtgaccaagg ggcagaccgc atcgtcatcc tacaagccag ctatcctctc
      421 caacggcgtg cgcacgctcg tgccgccgca tatcgggacg ggaacgcgga tcgtgatcat
      481 gaccgaggac ggctcgtacg tcgagcgcgc caaggactag tcggggatta gaggacgggg
      541 cacgcattgc cgcttggggg cgacgcatta ggtacagaag ctgtccggtt agcttcgcgg
      601 ctgctggcat cagtggttgt gctgcttgcc ggcacggtgg tccctgacgc cgacgaattt
      661 cgcaccgcgg ccatttcggc ggtccgggtc gaatggcgcg ctgcgctcga tcagttcaga
      721 tccgagatca acacccggcc cgcggtcgcg tccgtcttca ccttctcagg ccaacgccgg
      781 ctgcccgcgt tcgatccgcg cacaacaccg gcgctggtgc aattgaacgc ggcgacctcg
      841 ccgatcttta cagggatcgg acgcagtccg gtaccggtgc tactgccctt cgagaccgcg
      901 gcatttcttg acgcgcgact aagcgccgcg ccggaaaccc tctcgctttc gcgctatcag
      961 gccgatttcc gttccgctga cttgtttgac gccggtccgg ccggctacga cgcgatgttt
     1021 gcgctggagc cgggcgccgg cgatggcatg ccgcagcgaa ccttcgccag gccggtcgaa
     1081 gtccagatca ccggctcgat cctgatctac gacatcagcg atcccctcgg cgacaagggc
     1141 gaaccggtca aggcattatc tgcgcaatat cccgacctgc gccgcttcat ccgcgaaggt
     1201 tatgtgcgct atgcctttac ccgtttcggc gttccctatg tggtgtcgat ccagtgcctc
     1261 gacagcgccc ctcgcgcggg gcggcttgcc tgccgcgagg cctatcccgt cgccgagcgt
     1321 tttctcaggg cgctgcgtat cgccggcggg caacccgcgc gaccgcgaat ggatattgcg
     1381 gccgtcattg ccgagcggcc ggaagcgcgg tcggccgatt tcacctatct gccgagcggc
     1441 gatatcatcc ccaacagcgg cgcgcgcaaa cagagcggcc acgccgattt caccgcttat
     1501 tcgcaaattc gatttccgct ggaaaaagcc ccggcattcg cccgctcgca atcgttcgga
     1561 aaccgcagat cgtacgaccg gccgctgatg cccgacgagc cggctggcgg aaacaccggc
     1621 tatccgtggc gggataattt ttgcgaagcc cgcagtttca gtgtgggaca gtgcgccagc
     1681 ggtttcggcc atcaggggca ggatatccgc ccggcctgtt caccaggtaa cgaaagcgcc
     1741 gatcgctgcg atccctatcg gcaggcggtt gtcgctgtcc gcgatggcgt gctgatccgc
     1801 tcgccacagc agcaggcggc gacgctgcag atcaacacgc gcaacgaaca tatccgcttt
     1861 cgctatatgc acatgaaccc gaggagcatg gatgccgacg gcattctgaa cggacgccac
     1921 gtcgacgaag gcgaaaaact cggtgtggtt tcgaattatc tggaccatcc caacggcacc
     1981 agccgccatc ttcatttcga tgtgcaggtg ttcacgcgcg acggctggct ttgggtcaat
     2041 ccctacgtca ccttggtctc ggcctatgag cgtttgattc acgggcgcgg gcgcgagatc
     2101 gggcccgaag tcgcagcgtt acctgcggta actcatgcct ccgccgcacc tgcggtagct
     2161 catccatcgc cgggcgatgc catcgattcc ggcacccagg aaggcgccga gaactgacca
     2221 aaattgccgc agacctgcgg caggatggcg ggttccaacg gcaatgaggc ctgccacgac
     2281 cgtccttgcg ccgctaggat gccgctatga aacagcctga ttctcggaaa ggcctgaccc
     2341 gccgcaccct gctgcaatcg ggtctcggcg ccggcgtcct gctcgcccgc cccatggcgg
     2401 cgtttgccac agcaccaccc ggcttcgatc aatggcgcga cggttttcgc gcccgggcgc
     2461 tggcgaaggg catctcggac gcgacctatg cgcgcgtgat gggccgcatc gaaccggaca
     2521 tgtccgtgtt caagcagatg cagaagcaac cggagttcaa cgagcagatc tggcaataca
     2581 tcaatcgccg cgtctccgac tggcgcatca ttgccggcaa ggaggcgctt aagaagaacg
     2641 aggcgctgtt cgcgcggatc gagcaggatt ttggcgtcga gcgcggcacg ctgctggcgc
     2701 tgtggggcgt cgagtccgcg ttcggcgatc ccctggtgca gcagaaccac atgcggccgg
     2761 tatttccctc gctggcggca ttggcgtgga acgagccgcg ccgccgcgcc tattgggaaa
     2821 ccgaactgat caacgcatta aagatcgtcg atcgcggctg gagcacgccg gaggagatgc
     2881 gcgggtcctg ggccggcgcg atggggcaca cccaatggat gcccgaggtc tggctcaacg
     2941 tcggaatgga ttacgacaag gacggccgcg tgtcgccgtt cggcaagccc gatgacgcgt
     3001 tgggttcaag cgcgcgcttt ctggtcacca gaggaaaata tcgccgcggc gagcattggg
     3061 gttacgaagt ccgcgcgccg ggcgcagcct ccagcggcag ccgctcttat gctgcgtggt
     3121 ccagcgccgg cgtctcgcgc gccgacggcc agcccttcgc gcagccgaac gcttccgcgc
     3181 agatgtgggt gccgacgcag ggcggtccgg ccttcctgct gggtccgaac ttctacgcgg
     3241 tgcgcagcta caatccatcg atgaactacg cgctggcgat ctgccatctc ggcgatcgca
     3301 ttctgggcgc gccgcccttc ctccagccct tccccggctc ggagcgcgcg ctgacgctcg
     3361 cagaggtgca ggaaatgcag gtccgattga caaaagcggg tttcgacacc ggcggcaccg
     3421 acggccgcgt cggcaacgat accatgcagg cgatcaggga ttatcaggcc aagatggggc
     3481 ttttgcctgc cgacggttac ggcggactga aggtgctggc gcggctgagg caggggtcgt
     3541 gatcgcttga acctcggcgc ttgtgcagag agcttggacc caacgcgtca gacaacgcac
     3601 caaacttgcg cgtacgcgct caaaaaaatt ccgcgcggtg gttgctactc cgtttcaacg
     3661 tcttccagac ggaccccgcg cccggcctgc agactatcgc gcgcttgctc gatccggcgc
     3721 aaaaatcgcg gatcgttttc gagccgatat tcaaaccaat cctcttccga cccgaacccg
     3781 atcagaatcc cagccggcct gccgtgacga gtgataatga tctcctgagt ctccgcctcg
     3841 cgaagatacc gagacagatc atccttgacc tccgagagcg gaacttcttt cactccggat
     3901 ttccgaactg tgcgagccat gactgagcct ccgatttggt cacgatcgcc aaaacctcga
     3961 cggtcgtctc ggttacgtcg tagaatattc gcacttcgcc gacacgaagc cgatattgcg
     4021 gtttgcgcag gccgcgcagt cgtttgatgc ggcttcgact tacttttctt ggctcctacc
     4081 tgatcatcgc cggcccctga tccggcaccg cgatatccag cacccggaac tgcacgcgct
     4141 cggtgccctg ccagcggtcg acgctcagcg atcccgcgac atgcagcggc tggccgcgat
     4201 gctgggtcag cgcgctgccg agtttttgtc cgatcgaacg aaacgcgatg ccgttcacga
     4261 ttgcgccgtc gccggatttg aaccgcaccc gtaagtgcgc ctggccgact tcatcggcat
     4321 agaccaattg atgcgacggc agcgcgatga cgggctcagg attgccgctg ccgaacggcc
     4381 cggcgcgatt gagcgtggct gcgaattccg gcgtcaccgc ccgcgcgctg acggcgccgt
     4441 cgatgaacag ctcgttttcg tgccgcgaat tggcgacgtc ggccgccagc gcgctttcca
     4501 tgtaggcgcg gaattccgcc aatctttcct tgcgcagcgt cacgccggcc gccatggcgt
     4561 gaccgccgcc cttcatcaac agaccgtctt tcacggcctg ccgcaccgcc ttgccgagat
     4621 cgacgccgga gatcgagcgc cccgaaccgg tgccgatgcc gcccggctcc agcgcgatgg
     4681 caaacgccgg ccgcgagaac ttttccttca gccgtgaagc gacaagcccg accacgccgg
     4741 gatgccagcc ttccgaggcc gtgacgatca cggagccctt gtcctcgagc ccgagcgagg
     4801 ccagcgcctc ggcttccgcc tgcgcctccg ccatctgttc gatgacacgg cgttcggtgt
     4861 tcagccggtc aagttcggcg gcgatcgctg cggcttcgga gatgtcgccc tcgagcagca
     4921 gccgcacccc gagatcggcg cgcccgatgc ggccgccggc gttgatgcgc ggccccagca
     4981 tgaagccgag atgccaggct tcgggcgggc cgttgagccg cgacacatcc atcagcgcgg
     5041 tatggccgac atggtcgcgc cgccgcatcg cgatcaggcc ttttgcaaca aaggcgcggt
     5101 tgagcccgat caatggcgcg acatcggcga cggtgccgag cgccacatga tgcagcatgc
     5161 ccagaaggtc gggctccggc atttcgccgg tccaaaaacc ccgcgcacgc aattcccggt
     5221 tgaccgcgac cagcgtgacc agcacaaggc ccaccgcggc gagatggccg agacctgaaa
     5281 ggtcatcggg ccggttcgga ttcaccagcg cgtcaacatc gggcaattcg tcgccgcttt
     5341 gatggtgatc gatgacgacc accgccatcc ccagccgtcg cgcttcggcc agcggctcga
     5401 aactcgtggt gccgcaatcc accgtcacca gcagcgtcgc gcccttgccg gcgagcatgc
     5461 gcaccgcctc gacattgggg ccgtagcctt cgaaaatgcg gtcgggaata tgaatcaacg
     5521 gatccagccc gcaatggcgc aggtgccagg tcagcagcgc cgccgaggtc gcgccgtcga
     5581 catcgtaatc gccgaagatc gcgacctttt cctttcggat ggccgcgtcc gcgatgcgtt
     5641 ttgcggccgt ttccatctgg gtcacggtgt cgggatcggg catcaatttg cggatggtcg
     5701 gatcgagaaa atcctcgacc gcgtcgatct cgacatcgcg ccccgccagc acccgcgcca
     5761 gcatctccgg caattgatag cgctgcgtga tcgcgagcgc acgcgcagca ccgcgggcgt
     5821 ccagccggtc gcgccacaat tttcccgtcg ccgaattcga cacgccaaga aacgccggcg
     5881 gcgcttcaat gggcagtgcg gatgcgggaa gcgtcatgat gccttcagta gatagggaag
     5941 acggtcgcgg gatcaatgat tcgcgcaact tggtcgcgac ttatgcccgt catccccgtg
     6001 tggccgctgg gtagttcgcc agaaaccgtc gtaccccgcg catgcggggt atccagtacg
     6061 ccgcggcttc ccggctccat cactgacgcc tcggaatact ggctcgccgg ccggagcctc
     6121 tcgtcgggcg gcgcgacgcg ccgaccggtg gcagacgata acggctgaga gcgtggcgcg
     6181 ttcgcattca tcacctacaa cttcgcgatc tcgcgacgct gcgcgcccga gccttgcatg
     6241 aatccttcgc cccaaaagag ggcgtgggga atgccgggtg cccgctgcac ccgcagcctc
     6301 gtgcgcgctt tggattgata gatagcacac gagtaaacga gtacaccgga atcgcccggc
     6361 attcccgcac gcaatggttt taacggctta tgtcgcgctc tccccggtga ccgggctctt
     6421 ttgccaccgt cgactcgcgg atatgttttt gtcactgccc ggtcgggccg acttaaactc
     6481 cgcgagactt gacgccagcg tcggggcgtc aggaccacac gattttgccg tccgcgaaag
     6541 cgtctctcgt cagcgtgccg gatatcgctc acaagtcttt cgacccaccc tgcgatccca
     6601 tcgcgcgcaa aacgctgccg cgtccaccgc atcccgcccc tacgtccgtg acgatcatga
     6661 tacgcccctc ttgtggggtg ggatggcgag agtgatagag gtgatttggg gtgtgcgaac
     6721 acagaaatat ttttgcaaag aggactggac tggtagaaac agactgaacc taaaggagaa
     6781 tttttcctcc acgcgcaacg gtcgccggtc atgatctcgg cggagacacg gcatgccggc
     6841 gctttttttt cgtggcggcc gtcgatcgct tcgctcatcc gggcaacgct tgctatcgcg
     6901 tccccggacg cggtgcggcg ccctcccggc gacgccaacg ggtcgcgcga atgcgcgccc
     6961 gatgacaggc tccgcgtcgt ccggtcgcgc tgcaccgcag ggcagattgt aatccgccgt
     7021 tcagcgacga tgaagaggcg ggttacgccg gagcctgcca tcgcgccgcg cgttgcgcgg
     7081 acccgttggg ctacaacccg cccttgccgg atttagctca atcaacacgg cgatgccgcc
     7141 ggtctcgccg tcccggcggc actgttttgc gcccggcgta atacgggttg accatacaca
     7201 gtgcggcgcg gcccgatgcc gaggcattgc actgtgccag cgaggtatag gcgcattcaa
     7261 agtaatagtc gttccagcct tggtagattt gcaggcagac cggataggcc gggtcataag
     7321 tctgggcgcg agccgacggc gctgccgaaa ccgtcgcaat cgtcaaaatt gccaaagccg
     7381 gaatgcgcat tcaaaatctc cctagcggct catgcgcgac ggcgcatgaa tacgccgaag
     7441 ccagcgcata tgccgccgtc atccctgcga aagcggggaa ccagtactca ccaacgcctg
     7501 cgtttactgg atccccgctt ttcgcgggga tgacggctgt gagtgttgcg ccgaagcaaa
     7561 atatctctaa caccccatcc gatccgcgat cccgccaaaa tccttcgcga caatatccca
     7621 atcgcccttc gcctcgaaat cgtatttttg caaggggccg tattcggtcg gtcgcgcgac
     7681 gaaggccgtt ttgagtccca gtttctgcgc ggccttcaga tcgtggttat gcgcggccac
     7741 catcatcacc tgctccggcg gcaagccaag cagctttgcg gcgccgaggt aggtctcggg
     7801 atcgggcttg tagtgttcga acagttccgc cgacagcacc aggtcccacg gcaggccggc
     7861 gaatttcgcc atgttggtca aaagcgcgaa attgccgttc gagagcggcg cgatgatgta
     7921 ttttgtcttc agccgcgtca gtccggcaac gctgtccggc cacgggttga gccggtgcca
     7981 gcccatggtg aggtgatgca ggtcggcgtc gttcaatcct tcaatcgaaa gctgcgcgac
     8041 cagtttttcc agcgattgtc gatgcagtct gtccaatatc acatagccgc gctcggggtg
     8101 ctttctcacc tcatccatcg acgcggcata gacggcgcgc cagccgtcaa ccaaagcggt
     8161 ccagtccgcc ttgacgccgc gtttgtcggc ccatttggtg aagtccgcga tcaggctggt
     8221 tcgccagtcg actacggtgc cgaacacatc gaacaccagc gccttcaccg cggaaatatc
     8281 cgacatcgca ctcatggctt cactcccgcg ttattgtttt tctgcatagc acacgtccgg
     8341 ctggctcgca tggaggcgct ttaaatcacg atctgttcca tgcaacaaca gcgatcaggt
     8401 aaccaagccg cgatgcgcga ggaacggctt cggttgcaac ctcgcagttt gcatcgtgaa
     8461 aaccaacaac gaacacactt tcgaaaacgc aacgagcgga cgctgaacca tccggctgct
     8521 tacgtcttac ttgacggtcc ggtcgcgctg aaccagagag cgctcgtgga aaccttgcag
     8581 cagcgccgcc cttgggtggt cctggtatct tgaccaccaa ggaagaatgc gacgtgtaga
     8641 tgcgcgccgt gggatgaggc caaggcgctg caaaggccgt tgcccgatgc tgacctgatg
     8701 attgttgcgc ggggtacgga cgaggaggac cgcgcggcag cggcatgatc agaacggcct
     8761 atcaaggtcc ggaagtcatt attcgatcac atcgttggca cgcgcgaggg ttttttgggg
     8821 gtgggatggt cgggtttaac ctttaattcg gctggatagc tgcgtgagtt tgctaaacaa
     8881 taaggcccat gtccaaatgg cttaaacagt ttctgtttgg gatttggggt cttctgctga
     8941 tcccactgat cacgcctatt ctcgaaaaat ggttggagga aaacgtcttc tccgatccta
     9001 acggtatggc cgcgacagtt tttcccaacg ttatggccac gaccgttttt agcaacctgc
     9061 tcgcactggg ccaacagcgt tggtttaaat ttgcgctggt ctttttgacc ggaatcgtaa
     9121 ttggcgtttc attagaatgg ttaaaccgga agtcggacga aaaaaaagct tccgagctcc
     9181 gaagcctcgg ctctaaattt cgcagtcttt cagataacat caaagttcgg accgcgtcgt
     9241 caggatggcc ggacaatgtg cgtgacctta aacctgcaat cctgtctgca tttatctccg
     9301 ccaagaaatt tgatttgtgg gtaccgaatg agcacgtgta ccaattgccg gatgcgtcat
     9361 tcctttgtga atacttcaga tgtgtcggaa ggcttttaga ggatggacat tttgacgagg
     9421 ccaaccgtga ggcgctctcc tggaaaccat ttctcgacaa agcaaagctg tcttgagttt
     9481 ccgtactagt ccggggatcg actcgaagaa ttgtcggtga agttgcctaa tagatatggc
     9541 gcaacccaag ttatatgtgt gtcaccggaa attttgacca cacaacaaac aggaggaatg
     9601 tccattacac acggaaatgt ccgcgtttcg acctgaggtc ggctttgctc caataagcag
     9661 acatcgccgg gtctccgctg gaaaccggtt cacatccacg tcgttgccct ccgcataagc
     9721 tggtcaggaa ccaatacgca tcccacaggg agccagcggt ttgccgttcg agcgatgttg
     9781 caccctagtc cgggtagtgg gtcacttttg attgcggcga acgattcggc ttgagccggc
     9841 tcgccgaagg cgcatattcc gtgaatggcg acaatcgtcc aatgcgattg cggtgcggag
     9901 tacaaacgca ctgaaacaaa gttcttggta ccgcacaccg gtcacgc
//
LOCUS       DASWGM010000907         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2338509,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000907 DASWGM010000000
VERSION     DASWGM010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2338509"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..2169)
                     /locus_tag="VGA15_24625"
     CDS             complement(295..2169)
                     /locus_tag="VGA15_24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9590913.1"
                     /translation="MSGNAAAALTKPAFRRIEWLERDIAVERRPDGVIVLKSRIPLRA
                     YEKHIPASLAKWAKEHPNRIWLAQRAGADRQWRKLSYGEAKRTVDALTQAILDLKLEP
                     GRPVAILSGNSIEHALMTQASMQARFPAAPVSPAYSLMSHDHVKLKYLFDLIKPAVVM
                     VQDGPTFEKALRAVDLDGVTVIHVARPCKGIKSVAFADLAATPVTTDVEESIANIAPD
                     TVGKLLFTSGSTGMPKAVINTQRMMCANAAMMMQVRPRGPNAPQATYLDWMPWNHTMG
                     GNALFNPVLTEGGTLYIDDGRPTPGMIDETLRNLREISPTYYANVPAGYAALAAAMEK
                     DDALCRSFFKNLGLMAYGGARLPDDLHDRMQALAVRATGERIVFYTGWGSTETAPTST
                     GTYWNTERVGLIGLPFPGVELKMVPVGSKYELRLRGVNVTPGYFDRPDLTKAAFDEEG
                     FYCIGDAGVFVDPSDPVQGIIFAGRVVEDFKLTTGTFVHVGSLRTDAIAAATPVVQDA
                     LVAGQDRPFVGLLAWPNLHACRQITGDPDASYEDVVKHPGVLACLRSGLQAHNASTEG
                     ASSLRIARAMLMTEPASIDGNELTDKGYINQRAGLERRAALVERLYADRPGEDVIVLQ
                     "
     gene            complement(2456..>2786)
                     /locus_tag="VGA15_24630"
     CDS             complement(2456..>2786)
                     /locus_tag="VGA15_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590914.1"
                     /translation="ASVAAYEGQIGQIAYSASKGGVVGLTLPAARDLASQKIRVNTIA
                     PGLFLTPLLMGLNEEARKSLGAQVPHPARLGDASEYGNLAVHIVENPMLNGETIRLDG
                     AIRMAPR"
BASE COUNT          482 a          889 c          859 g          556 t
ORIGIN      
        1 gctccttgcc gtcgagcacg acgcgcaccg ccttgggcgg gcacttttcg gcaagaaatt
       61 ttcgcgcttc gtcgcggagt tgtttttgtt cgtcggagaa atcgaaattc atggcgtctt
      121 actcgggttg ggtgaggtgt agttttaata ggtcggtcgt cgtcccggcg aaggccggga
      181 cccataacca ccggcgttta tgtcttggct cggatttggc tccaacctga catcgataat
      241 cctcggcgta tgggtcccgg ccttcgccgg gacgacggtg gcaggttgtt gaattcattg
      301 cagcacaatc acatcttcgc cggggcgatc ggcgtagagc cgttccacca gagcggcacg
      361 gcgctcgagg ccggcgcgct gattgatata tcccttgtcg gtgagttcat tgccgtcgat
      421 cgaggccggc tcggtcatca gcatcgcacg cgcgatccgt aggctgctgg caccttcggt
      481 ggatgcgtta tgtgcctgca agccactcct gaggcatgcc agcacacctg ggtgtttgac
      541 gacatcctca tagctcgcgt cgggatcgcc cgttatctgg cggcaggcgt gcagattggg
      601 ccaggccagc aatccgacga acgggcggtc ctgccccgcg accagcgcgt cctgcaccac
      661 cggcgtggct gctgcgatgg cgtcggtgcg caatgagccg acgtgcacga aggtgccggt
      721 cgtcagcttg aaatcctcca ccacgcgccc tgcgaagatg atgccctgca cggggtccga
      781 aggatcgacg aacacgccgg cgtcgccgat gcaataaaag ccttcctcgt cgaacgccgc
      841 cttggtgaga tcgggccggt cgaaatagcc gggcgtgacg ttgacgccgc gcagacgcag
      901 ttcatatttc gatccgaccg gaaccatctt caactcgacg ccgggaaacg gcagcccgat
      961 cagcccgacg cgctcggtgt tccaataggt cccggtcgag gtcggcgcgg tctcggtcga
     1021 gccccagccg gtatagaaca cgatgcgctc gccggtggcg cggacggcca gcgcctgcat
     1081 ccggtcgtgg aggtcatccg gcagccgcgc gccgccatag gccatcaagc ccagattctt
     1141 gaagaagctg cggcacagcg cgtcatcctt ctccatcgcc gcggcaagcg cggcatagcc
     1201 ggccggcaca ttggcgtaat aggtcggcga tatctcgcgc aaattgcgca gcgtctcgtc
     1261 gatcatgccc ggcgtcggcc ggccatcgtc gatatagagc gtgccgcctt cggtcagcac
     1321 cggattgaac aacgcattgc cgcccatggt gtggttccac ggcatccagt cgagatacgt
     1381 cgcctgcggc gcgttcggac cgcgcggccg aacctgcatc atcatcgcgg cgttggcgca
     1441 catcatccgt tgcgtgttga tgaccgcctt gggcatcccg gtcgagcccg aggtaaacag
     1501 cagttttcca accgtatccg gcgcgatgtt cgcgatcgat tcctcgacat ctgttgtcac
     1561 aggcgttgcc gcaaggtcgg cgaaggcgac gctcttgatg cccttgcaag gacgggcgac
     1621 gtgaatgacg gtgacgccgt cgagatcgac cgccctcagc gccttctcga aggtcgggcc
     1681 gtcctgcacc atcacgaccg ccggcttgat caggtcgaac agatatttca gtttgacgtg
     1741 atcatggctc atcagcgaat aggccggcga caccggggcg gccggaaacc gcgcctgcat
     1801 cgaggcctgc gtcatcagcg cgtgctcgat cgaattgcca gacaggatcg cgaccgggcg
     1861 gccgggctcg agcttcagat caaggatggc ttgcgtcagc gcgtcgaccg tgcgtttggc
     1921 ctcgccatag gacagtttgc gccactgccg gtcggcgccc gcgcgctgcg ccagccaaat
     1981 ccggttcgga tgttccttag cccacttcgc cagggaagcc gggatatgct tctcataagc
     2041 cctcagcgga atccgcgact tcaggacgat gacgccgtcg ggccgccgct cgaccgcgat
     2101 atcgcgttcc agccattcga tcctgcgaaa ggcaggtttt gtcaaagccg cagctgcatt
     2161 cccactcatg tctcgtttcc caaagccact tggtctcttt tcggaccctt tttcttgttt
     2221 tgcgtatcca ccggatcgcg gcgtttatta ttgcgccctc tccccttgtg ggagagggca
     2281 tcaccggcag tagcttcaca tttagctggg tgaggggtct ctttctatcg atagtccgca
     2341 acccctcacc cgtcagcctt cgcatagccg atgcagcgca tcggcgttct tctaagaacg
     2401 gcggccaacg gccgcctatg caccctctcc cacaagggga gaggggaaga aaaagctacc
     2461 ttggcgccat gcggatggcg ccgtcgagcc ggatggtctc gccattcagc atcggatttt
     2521 cgacgatatg caccgcgagg ttgccatatt cggaggcgtc cccaagccgc gccggatgcg
     2581 gcacctgcgc gccgaggctc ttgcgcgcct cttcgttcag tcccatcaac agcggcgtca
     2641 ggaacaggcc gggcgcgatg gtgttgacgc ggatcttttg gctggcgaga tcgcgcgccg
     2701 ccggcaacgt caggcccacc acgccgccct tggaggccga gtaagcgatc tggccgatct
     2761 ggccttcata ggccgcgacg ctcgcg
//
LOCUS       DASWGM010000908         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1071909,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000908 DASWGM010000000
VERSION     DASWGM010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071909"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VGA15_24635"
     CDS             <1..349
                     /locus_tag="VGA15_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9590915.1"
                     /translation="DHQFIHVDPERAKRETSYGTTVAHGFLTMSLMSIMSYEVMPVIE
                     GTTMGVNYGFDKLRFISPVRAGSRVRGHFTLVEAKLRKPKELQSRTNVTVEIEGEEKP
                     ALVADWIGLIYFA"
     gene            495..1412
                     /locus_tag="VGA15_24640"
     CDS             495..1412
                     /locus_tag="VGA15_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590916.1"
                     /translation="MTIRFDGRVAIVTGAGNGLGRAHALGLASRGAKVVVNDFGGARD
                     GTGGSLTPAEAVVEEIRKAGGEAVADGADVSNFEQVKAMVAKATEKWGSVDLLCANAG
                     ILRDKSFAKMEIADFAKVLDVHLNGTFYCCKAVWDGMRERNYGRIVVTTSSSGLFGNF
                     GQANYGAAKTGMVGLMNVLAEEGRKNDIRVNMISPTAATRMTEELLPPQALALMKPEA
                     ITPAVLYLLGEDAPTRTIMGAGAGSFAVIKIMETEGINLGQSDWTPEAVSAHFAEISD
                     MAKAKALEGAFQQTQKYVGQAAARAGIKL"
     gene            1534..>2624
                     /locus_tag="VGA15_24645"
     CDS             1534..>2624
                     /locus_tag="VGA15_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEX9590917.1"
                     /translation="MLRTQVAIIGAGPAGLMAAEVLAQGGAAVTVYDAMPSAGRKFLM
                     AGRGGLNLTHGEPLPEFLARYREAMPNLRAAITAFPPQALREWSEALGQPTFVGSSGR
                     VFPEAFKASPLLRAWLRRLDSMGVQFAMRHRWTGWDDDGQLSFRTPDGQRLVDARATV
                     LALGGASWPRLGSDGGWVEMLGARGVVVSHLQPANCGFTVSWSEIFRDRFEGHPLKGV
                     VLSFGSHAARGEAVITRTGIEGGAIYALSAGLREAILNTGQATLRIALRPDLEMKDLV
                     ARLSAPRAKQSFANWLRKTAHLSPVAIGLLQEAAIASGMSLASLSPTALARLINAVPI
                     ELNGVAPIARAISTAGGIAFSELDADFMI"
BASE COUNT          488 a          782 c          875 g          479 t
ORIGIN      
        1 ggatcatcag ttcatccatg tcgatcccga gcgcgccaaa cgcgaaacct cttatggcac
       61 gacggtggcg cacggattcc tgaccatgtc gctgatgagc atcatgtcgt atgaggtgat
      121 gccggtgatc gaaggcacga cgatgggagt gaattacggt ttcgataaac tgcgattcat
      181 ttccccagtg cgcgcgggct cgcgcgtccg cggccacttt acgcttgtcg aagccaagct
      241 gcgcaagccg aaggagctgc agtcgcgtac caatgtcacc gtcgaaatcg agggcgagga
      301 aaagcccgcg ctggtggccg actggatcgg cttgatttat ttcgcttaac caccaaccgt
      361 cattgccggc ttgacccggc aatccatcac tgatcaaaaa tgtttttctt ttgatggatg
      421 cccggatcat ccccgatcgg gtcggggaca ggcccccggg catgacgaca gaaccggatc
      481 cgggaatttt actcatgaca atcaggtttg acggacgcgt cgctatcgtc accggcgcgg
      541 gcaatggtct tggacgcgcg cacgcgctgg ggctggcaag ccgcggcgcc aaggtcgtgg
      601 tcaacgattt cggcggcgcg cgcgacggca ccgggggctc gctgacgccg gccgaagccg
      661 tggtcgagga aatccgcaaa gcgggcggcg aggcggtggc cgacggcgcc gatgtctcga
      721 acttcgagca ggtcaaggcg atggtcgcca aggccacgga aaaatggggc agcgtcgatc
      781 tgctctgcgc caatgccggc attttgcgcg acaagtcatt tgcgaagatg gagatcgctg
      841 atttcgccaa ggtgctggat gtccatctca acggtacctt ctattgctgc aaggcggtgt
      901 gggacggcat gcgcgagcgc aattacggcc gcatcgtcgt caccacgtcg tcgtcaggcc
      961 tgttcggcaa tttcggccag gccaattacg gcgcggccaa aaccggcatg gtcggcctga
     1021 tgaatgtgct ggcggaagag ggccgcaaga acgacatccg cgtcaacatg atttcgccga
     1081 ccgcggcgac gcgcatgacg gaagaactgc tgccgccgca ggcgctggcg ctgatgaagc
     1141 cggaagcgat cacgccggcg gtgctgtatc tgctcggcga agacgcgccg acccgcacca
     1201 tcatgggcgc gggcgccggc tccttcgcgg tgatcaagat catggagacc gaaggcatca
     1261 acttaggcca atccgactgg acgcccgagg cggtgtcggc gcatttcgcc gaaatcagcg
     1321 acatggcaaa agcaaaggca ctggaaggcg cgtttcagca gacccagaaa tatgtcgggc
     1381 aggccgcggc cagggcgggg atcaagctgt gacgtcattc cggggcgcgc gtgagcgcga
     1441 gcccggaatc cataaccacg acagggcgca tggattccgg gcctccgcca agcggcggat
     1501 ccacgatgtg caattgcaca tcggggaatg acgatgctta gaacccaggt cgccatcatc
     1561 ggcgccggtc ccgccggact gatggcggcc gaagtgctgg cgcaaggtgg cgccgccgtc
     1621 accgtgtatg acgcgatgcc gtcggcaggc cgcaagtttc tgatggccgg gcgcggcggg
     1681 ctcaatctga cgcatggcga gccgctgccg gaatttcttg cgcgctaccg cgaggcgatg
     1741 ccgaaccttc gagccgcgat aacagcgttt ccaccgcaag ccctgcgcga atggagcgaa
     1801 gcgctggggc agccgacttt tgtcggctcc agcggccggg tttttccgga agcgttcaag
     1861 gcctcgccgc tgctgcgggc gtggctgcgg cggctcgact cgatgggcgt gcagttcgcg
     1921 atgcgccatc gctggaccgg ctgggatgac gacggacagc tttcttttcg cactccggac
     1981 ggacaacgct tggtcgacgc ccgtgcaacc gtgctcgcgc tcggcggcgc aagctggccg
     2041 cgactcgggt ccgacggcgg atgggtggaa atgcttggcg ccaggggcgt ggtggtatcg
     2101 cacttgcagc cagccaattg cggattcacg gtctcatggt ccgagatttt tcgcgaccgt
     2161 tttgagggcc atccgctgaa gggtgtcgtg ctgtcgtttg gttcgcacgc tgcgcgcggc
     2221 gaggccgtga tcacgcggac gggcatcgag ggcggcgcga tctatgcgct ctcggctggc
     2281 ttgcgcgagg ccattttgaa tacgggacag gcgacactgc gcattgcttt gcgacccgac
     2341 ctcgaaatga aagacctggt cgcgcggctg tcggcgccgc gagcaaaaca atcatttgca
     2401 aactggctgc gcaagactgc gcatctgtcg cctgtggcca tcggcctgtt gcaggaggca
     2461 gcgattgcgt ccggcatgtc gctggcgtcg ttatcgccca cagccctcgc ccgattgatc
     2521 aacgccgtac cgattgagct caatggcgtg gcgccgatcg cgcgcgcgat ttcgaccgcg
     2581 ggcggaattg ctttcagcga actcgatgcc gacttcatga tcca
//
LOCUS       DASWGM010000909        12703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2196268,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000909 DASWGM010000000
VERSION     DASWGM010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196268"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VGA15_24650"
     CDS             <1..581
                     /locus_tag="VGA15_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9590918.1"
                     /translation="AHPFTRHPGGFVNREGEVHPVIQAALDRGLRVDVGHGSHFSYRL
                     ARKAIGAGIIPTTLGADIHGYNTHVPAPAGTPDEHADDENHPFASAAKFSLVQAMSSM
                     MALGLTLEQVVPMVTSHPAEMLGLSDTIGALRPGMEADVTVLTEKPGRYILSDNEKTE
                     VVADSLLQPVFCLRAGVRHDAVAPILPRAVAA"
     gene            617..3004
                     /locus_tag="VGA15_24655"
     CDS             617..3004
                     /locus_tag="VGA15_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9590919.1"
                     /translation="MSHVESDPASDSMADTGQGVGARLLRKEDDRFMRGRGQYVADIR
                     LAGLQDVAFVRSPLAHARIRAIHVPERYRDVVFTAADLTGVKPIRAVSGLPGFKISEQ
                     PVLATGKVRHVGELVAMCLAPTRAEAEDIAAAVTLDLEELPAVHDMRKARDGDTALVH
                     EHWGDNVFLESAFEVDIAPAFDAPIKVTREISTARQCMSPLEGRGVVATFDHRLDQLT
                     LYTGAQMPHIVRNGLSDCLGIEQGQIRIVSPDIGGGFGHKGILLPEEICLAWLTMRKG
                     RAVRWIEDRREHLTASANCREHFYKITVYADRDGRLRGIDCEATVDSGAYSSYPFSAC
                     LEAAQVASILPGPYRMPAYRCRTFSVATNKCPILPYRGVARTGVCFALEIMLDAVAVA
                     AGIEPGEVRLRNLVGPDEMPFDNITKKHFDSGDYPQAMRRALAAIGIAAVRARQRTQE
                     PDGRLVGVGLSIYCEQAAHGTSVYAGWGIPMVPGHEQANARMTPDGGLEIRVGVHSHG
                     QGLETTLAQVAHEILGIDTKKVRVVHGDTAITPYSTGTWGSRSMVMAGGAVATACIEI
                     GERAKLIGAKLLQLDPTLVSLRDGRVCGPNSSIGLAEIAHTWYRRPQDLPPDVDPGGL
                     ESTSGYKPRRDSGTFSYAAHAVTVAVDPDLGDVEILDYVIVEDGGVLVNPMIVDGQIY
                     GGLAQGIGTALYEEMPFDSAGQPLATTLADYLLPGPTEVPEPRLDHMETPSPYTMFGV
                     KGIGEGGAIAPPAAIANAVNDALRPLGVELLHSPISPRRVVEAILAAREAERSAA"
     gene            3001..3837
                     /locus_tag="VGA15_24660"
     CDS             3001..3837
                     /locus_tag="VGA15_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9590920.1"
                     /translation="MKAAAFAYERPADLNAALALMANADGMTKIIAGGQSLGPMLNLR
                     LVEPDLIVDISGLSELKQAERCGDELVIGACITHGDIEDGRIPDVTRGAMQRVAGAIA
                     YRAVRNRGTIGGSLSHADPAADWISALAALGAKVTLRSLTGARDLAIEDFVTGALESA
                     LQSGEIVEAVRVPTMTPSAKWGYIKACRKTGEFAHAIAAVLIDPESASARAVIGAVEA
                     PPIVVADAAALFGGRVTADFKRQFDARAADAILIKAGIANATDRHIHVAVLRRAIVEA
                     AT"
     gene            3834..5027
                     /locus_tag="VGA15_24665"
     CDS             3834..5027
                     /locus_tag="VGA15_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9590921.1"
                     /translation="MTTIALTVNARAVKASVEPRTHLADFLRDDLDLTGTHLGCEHGV
                     CGACTLLLDDMPARSCITYAVACDGARVTTIEGLDDDEITIELRAAFTREHALQCGYC
                     TPGMLIAARDLVLRLAAPDERQIRVGLSGNLCRCTGYVGIVRAVRAVIDQRRQRGIMA
                     VPGGGRTVLGPAGSGNGATSTSDIAQVPAAEAELRSENSGAIAIPDFTPVKVFEDHFT
                     VAYPPERVFDMFGDVTQVAACLPGASLVAVPTPERVEGVIRVKLGPISANFHGAARIE
                     RNSENLSGRIVGAGSDQRSRSSTQGEIRYHLVPVEQGTATRVELSIGYSLRGMLAQIA
                     REGLVRDLAARLTAEFARNLERQLSGAPSDGSAAAAQNLNGVSLLMGLLGMRARKALR
                     RVFGR"
     gene            5097..6518
                     /locus_tag="VGA15_24670"
     CDS             5097..6518
                     /locus_tag="VGA15_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX9590922.1"
                     /translation="MTNSELRADTHSIEPIPDADRDSTGPQQMWIWAGANIAPVNWAL
                     GALGIILKLGLWETIGVIVVGNLFGCAIFAAFTVMGHKTGVNQMVLSRSAFGRRGAYL
                     PSALMFLMTLGWIGVNTYFPVKISMAILGQFGIADTLVTTFIVITIVMVLQVVIGIYG
                     FYAIRTFEKYTVPPTVAIMVLMSVLAWSQPGVVNWHLASTLPPGAHLAMLTLLMSAIG
                     VGWGISWVTWASDYSRFVPRSVSSSSVFWYSYVGMFVPSVWLAILGATIASVTLDTDP
                     AKMVSAVFGGVASLLVLLMVLHGPIATNILNVYSAALAALSMGLRLSRTALALIVGIA
                     GYLVTVYFIFAPSFAKAFDNWMISLLLWMSPWAGVVMADFFIKRKGQIDVAELYRSPE
                     TSAYGDINWGGTVAFFAGLIAGWSVQDGLVPALQGPISTGLLAGADLSWLFGIVVSGA
                     VYLALSRRAVATSSAVATGTAAQ"
     gene            6678..6935
                     /locus_tag="VGA15_24675"
                     /pseudo
     CDS             6678..6935
                     /locus_tag="VGA15_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
     gene            complement(6994..7332)
                     /locus_tag="VGA15_24680"
     CDS             complement(6994..7332)
                     /locus_tag="VGA15_24680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590923.1"
                     /translation="MPIENHRSNGDVSSPGLDSRHLDIACGASGAGHHRNAGLGLFGL
                     LFRIASWMAAEILAGCAAYAEAMYPMYTQQDEHPDSDDRAGSQQTAKVIPIKGASESR
                     SPPDIQGRKG"
     gene            complement(7551..8525)
                     /locus_tag="VGA15_24685"
     CDS             complement(7551..8525)
                     /locus_tag="VGA15_24685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX9590924.1"
                     /translation="MLLSCGDALIDFLPVKSVDGRDALVPVAGGSCLNIAVGMARLGA
                     PAGFVGGISTDLFGRMIADHALASEVELRYATRSEHQTTLAFVRTVAGEPQYAFYDEG
                     TASRNWIYRHGSIPFDQIEAVHVGSTTLADDSGAAQALAMLEDTRGSVTISFDPNCRP
                     KLVRHKARYVERMNAFAAIADIVRMSDVDFEFLYGGSDYSGTANSLIEAGASLVVVTR
                     GIRGAQAWHKEAGQVEVEAPTVEVTDTIGAGDSFQAALLFALRAIGRIATGSLAQANS
                     GELRRALSFASTCAAFTCGRAGADPPRETDVGAGLSRLLDPLERTAPQ"
     gene            complement(8530..10194)
                     /locus_tag="VGA15_24690"
     CDS             complement(8530..10194)
                     /locus_tag="VGA15_24690"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX9590925.1"
                     /translation="MRQAFIGIDVGTSSARAGVFDETGKLLATARHPITVWHEAGSVV
                     EQSSSEIWAACAASVRAAMAEAALPPSAVKGLGFDATCSLVVLDAGANPLTVSASGDE
                     RRNIIVWMDHRATAEARFVNNTHDDVLRYVGGSISPEMEIPKLLWLKRHLPSTYNRAG
                     HFFDLADYLSFRATGSTLRSICTLACKWNFLAHEQRWSGSYFERIGLGDIASDSYARI
                     GKQIVAPGTPLGTGLTESAARDFGLLEGTPVGASLIDAHAGGVGTIGGRARSGQPVDV
                     RRRLAYIMGTSACIMATTSEPCFVPGVWGPYYSGMVPGFWLNEGGQSAAGAAIDHLVR
                     SHPAYNEAAATAHAGGMEVLEFLERRIVARSQNPGEAAWFARDIHVLPEFLGNRSPFA
                     NPDSRAVVAGMDLDADIGSMERLFVAGLCGLAYGLADVVDTFRSHGVDSDLMVISGGA
                     GRSSLVRQIMADTTGLTVAVPETQEPVLLGAAMLGAVAGSSYGSIGEAMASMSAIGCL
                     SEPTAPGMTEFHRTKRQVHGLMRKLDRESRDAMHGIELIAGVDTKN"
     gene            complement(10223..10996)
                     /locus_tag="VGA15_24695"
     CDS             complement(10223..10996)
                     /locus_tag="VGA15_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590926.1"
                     /translation="MYLEKFKLDRKTAVVTGAGQGIGLACAEALAEAGAMVVIADRDP
                     GAADAGRANLKAKGLNAEIVTMDVTDSARVSEVADQLASRYGKIDILVNNAGIARSET
                     PAEKVTDEHWLNVVDVNLNGTFWCCRAFGKHMLHAKSGSIVNIGSMSGFIVNKPQEQS
                     YYNASKAAVHHLTKSLAAEWGGRGVRVNAVAPTYVTTPLNAFVKNNPQMYDAWIGGTP
                     MARMGEVDEIASVVLFLASDAASLMTGSVVLVDGGYTCW"
     gene            complement(11064..12068)
                     /locus_tag="VGA15_24700"
     CDS             complement(11064..12068)
                     /locus_tag="VGA15_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590927.1"
                     /translation="MGQITLQGVRKSFGPVNIIKDANLDIEDGAFVVFVGPSGCGKTT
                     LLRLIAGLEDVTGGKILIDGRNVVDVPPAKRGLSMVFQSYALYPHMSVRGNIAFGLKM
                     AGLPRQEIDRKVEAAAATLNLTPYLDRKPRDLSGGQRQRVAIGRAIVREPKAFLFDEP
                     LSNLDAALRVQMRLEVTKLQKQLGTTAVYVTHDQVEAMTMADKIVVLNAGRIEQYGSP
                     LELYEQPANLFVAGFIGSPKMNFVSGEAAGEPGIAILGIRPEHLKIGKEGEGWPGTVS
                     VAEHLGSDTFLYVDAGKLGTLTVRAIGEFSLKAGDRVWLSPDPARLHRFDKDGGVIRA
                     "
     gene            complement(12091..>12703)
                     /locus_tag="VGA15_24705"
     CDS             complement(12091..>12703)
                     /locus_tag="VGA15_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9590928.1"
                     /translation="NSILIAGGSTLIAMLIAVPAAWSMAFAPTKRTKDVLLWMLSTKM
                     MPPVGVLVPIYLIYRDFGMLDTRAGLVMILCLGNLPIVIWMLFTYFKEIPKDILEAAR
                     MDGATIGRELVYVLAPMAIPGLASTLLLNFILAWNEAFWTLNLSTLDAAPLTVFIASY
                     SSPEGLFWAKLSAASTLAIAPIIVLGWFTQRQLVRGLTFGAVK"
BASE COUNT         2352 a         4025 c         3938 g         2388 t
ORIGIN      
        1 tggcccatcc gttcacgcgg catcccggcg gcttcgtcaa tcgcgaaggc gaagtgcatc
       61 cggtcatcca ggcggcgctc gaccggggct tgcgggtcga tgtcggccac ggcagccact
      121 tctcgtaccg tctggcgcgc aaggccatcg gcgcaggcat cattccgacc acgttgggcg
      181 ccgatatcca cggctacaat acccatgtgc cggcaccggc agggacgccg gacgaacatg
      241 ccgatgacga aaaccatccc ttcgcaagtg cggcgaaatt cagcctggtg caggcgatga
      301 gttcgatgat ggcgctgggc ctcacgctgg agcaggtggt gccgatggtg acgtcgcatc
      361 cggccgaaat gctgggcctg tcagatacga tcggcgcgtt gcgtcctggc atggaggccg
      421 acgtcaccgt gctgacggaa aagcccggcc gctacattct aagcgacaac gagaagacgg
      481 aagtcgtcgc cgacagtctg ttgcagccgg tgttctgctt gcgcgctggc gtgcgccatg
      541 atgcggtcgc cccgatattg ccgcgggcgg tagccgcata gcagcgcgcg acgaatagag
      601 gtggagacgg ccatcaatgt cgcacgttga aagcgatcct gcttcggact cgatggccga
      661 taccggacaa ggcgtcggag cccggctgct tcgcaaggaa gacgatcgct tcatgcgcgg
      721 gcgcggccag tacgtggccg atattcgtct cgccggcctg caggacgtcg cctttgttcg
      781 cagcccgttg gcacatgcgc ggataagggc catccacgtg ccggaacgtt atcgcgatgt
      841 cgtgttcacg gctgcggatc tgaccggggt caaaccaatc cgcgccgttt cgggtttgcc
      901 cgggttcaag atatcggaac aacccgtgct cgccaccggt aaagtgcgtc acgtcggcga
      961 actcgtcgcg atgtgcctgg cgccgactcg ggcggaagcc gaggatatcg cggctgcggt
     1021 gacgttggac cttgaggagc ttccggccgt ccatgatatg cgcaaggcgc gtgacggcga
     1081 caccgcgctt gtccatgagc actggggtga caacgtcttt ctcgaaagcg ccttcgaggt
     1141 cgatatcgca ccggccttcg atgcgcccat caaggtgacg cgcgaaatat cgaccgcgcg
     1201 ccaatgcatg tccccgctcg aggggcgcgg cgtggtggcg accttcgatc atcgcctcga
     1261 ccagctcacg ctctacaccg gcgcccagat gccgcacatc gttcgcaacg gcctgtccga
     1321 ttgcctgggc atcgaacaag gccagatccg gattgtctcg ccggacatcg gcggcggatt
     1381 tggtcacaag ggaatcctgc tgcccgaaga aatctgcctt gcctggctta cgatgcgcaa
     1441 aggccgcgcc gtccgctgga tcgaggatcg gcgcgagcat ctgacggcga gcgccaattg
     1501 ccgcgagcac ttctacaaga tcaccgtcta tgccgatcgc gacggccgcc tgcgcgggat
     1561 cgattgcgag gcgaccgtgg attccggcgc ttactcgtcg tatcctttct cagcctgcct
     1621 cgaggccgcg caggtggcga gcatcctgcc gggtccttac cggatgccgg cctatcgctg
     1681 ccggaccttt tcggtcgcta ccaacaaatg tccgatcctg ccctatcgcg gtgtggcccg
     1741 caccggcgtc tgcttcgcgc tggaaatcat gctggatgcg gtggcggtgg cagccggcat
     1801 tgaacccggc gaggtccggc tgcgcaatct ggtcggcccg gacgaaatgc cgttcgacaa
     1861 catcaccaaa aaacatttcg acagcggcga ttatccgcaa gccatgcggc gcgcgctggc
     1921 ggccatcggc atcgcggcgg tgcgggcgcg tcagcgcaca caagaaccgg acggacggct
     1981 ggtcggcgtt ggcctctcga tctattgcga gcaggccgcg catggcacgt cggtgtatgc
     2041 cggctggggc attccaatgg tgcccggaca cgagcaggcc aatgcgcgca tgacgccgga
     2101 tggcgggctc gaaatccggg tcggcgtgca ttcgcatggc cagggtcttg agacgacgct
     2161 ggcccaggtc gcgcacgaaa ttctcggcat cgataccaaa aaagtgcggg tcgtgcatgg
     2221 cgataccgcc atcacgcctt attcgaccgg cacctggggt tcgcgttcga tggtcatggc
     2281 cggcggcgcg gtggccaccg cctgcattga aatcggcgag cgcgccaagc tcatcggcgc
     2341 caagcttctt caattggatc cgacgctggt tagtttgcgc gacggtcgcg tgtgcgggcc
     2401 caacagcagc atcggcctcg cggaaatcgc ccacacctgg tatcggcgtc cgcaggatct
     2461 gccgcccgac gtcgatccgg gaggcctgga gtcgaccagc ggctacaagc cgcggcgtga
     2521 cagcggcacc ttcagttacg cggcccacgc cgtgaccgtc gcggtcgatc cggatctcgg
     2581 cgacgtcgag attctcgact atgtcatcgt cgaggacggt ggcgtgctgg ttaatccaat
     2641 gatcgtcgac ggccagattt atggcggtct tgcgcaagga atcggcacgg cgctctacga
     2701 ggaaatgccg ttcgacagcg ccggacaacc gctcgcgacg acgctggccg actatctgct
     2761 tccgggtccg accgaggtcc ccgagccccg tctcgatcac atggaaacgc cgtcgcccta
     2821 cacgatgttc ggcgtcaagg gcatcggcga aggcggcgcc atcgcgccgc cggcagcgat
     2881 cgccaacgcc gtcaacgacg cgctgcggcc gcttggggtt gaactcttgc attcgccaat
     2941 ttcgccgcgc cgggtcgtcg aggcgatcct ggcggcgcgt gaagcggaaa gatcggccgc
     3001 atgaaagcgg cagcgttcgc gtatgagcgc cctgccgatc tgaatgccgc gttggccttg
     3061 atggccaacg cggacggcat gaccaagatc atcgccggag gtcagtcgct cggcccgatg
     3121 ctcaacctgc ggctggtcga gccggatctg atcgtcgaca tatccggact ttccgaactg
     3181 aagcaggcgg aacgttgcgg cgatgaactc gtcatcgggg cctgcatcac ccatggcgac
     3241 atcgaggatg gacgaatacc cgatgtaacg cgcggcgcca tgcagcgggt tgccggcgcc
     3301 atcgcctaca gggcggttcg caaccgcggc acgatcggcg gctcgctcag ccacgccgat
     3361 cccgccgcgg actggatttc agcgttggcg gcgctgggtg caaaggtcac gctgcgcagt
     3421 cttacaggag cccgtgatct cgccatcgag gattttgtca ccggcgcgct ggagtccgcg
     3481 ttgcagtctg gcgagatcgt cgaggccgtc cgcgtgccga cgatgacgcc gtcggcaaag
     3541 tggggctaca tcaaggcttg ccgcaagacg ggagagttcg cgcatgcgat tgccgcggtg
     3601 ctgatcgacc cggagtcggc cagcgcacgc gcggtcattg gcgcggtgga agcacctccg
     3661 atcgttgtcg ccgacgccgc ggcgcttttt ggcgggcgtg ttacggccga cttcaaacgg
     3721 caattcgatg cgagagcggc cgacgcgatc ctgatcaaag ccggcatagc aaacgcaacc
     3781 gaccgccaca tccatgtcgc agtgctgcgc cgtgcgatcg ttgaggccgc cacgtgacca
     3841 cgatcgcgct caccgtgaac gcgcgcgccg ttaaggcctc cgtggagcca cgcacccatc
     3901 tcgcggattt tttgcgcgac gatcttgatc tcaccggcac ccatctcggt tgcgagcacg
     3961 gcgtatgcgg ggcctgcacc ctgctgctcg acgacatgcc ggcgcgttcc tgcatcactt
     4021 acgccgtcgc ctgcgacggc gcgcgcgtca ccacgatcga ggggttagat gatgatgaaa
     4081 tcaccatcga gcttcgcgcc gcctttaccc gcgagcatgc cctgcaatgc gggtactgta
     4141 cgccggggat gctgatcgcg gcccgcgacc tcgtgcttcg cttggccgcg ccggacgagc
     4201 gccagatacg ggtcgggctt agcggcaatc tctgccgctg caccggatat gtcggtatcg
     4261 tgcgggccgt ccgcgccgtg atcgaccaac ggcgccagcg agggattatg gcggtgcccg
     4321 gcggcggccg cacggtcctc ggccccgcgg gatcgggcaa cggcgcgact tccacttcag
     4381 acatcgcaca agtgccagcg gcagaagccg aactgcgctc cgaaaacagc ggcgcgatcg
     4441 ccattccgga cttcacaccc gtgaaggttt ttgaggatca tttcacggtc gcttatccgc
     4501 ccgaaagggt tttcgacatg ttcggcgatg tcacacaggt tgccgcctgc cttcccggcg
     4561 cgtcgctcgt cgccgtgccg acgccggaac gggtggaagg cgtgatccgc gtcaagctcg
     4621 gcccgatctc cgcgaatttt cacggtgccg cacgtatcga gcgcaacagc gaaaatctgt
     4681 cgggtcggat cgtcggagcg ggcagcgacc agcgcagccg gtcttccacg caaggcgaga
     4741 tccgctatca tctggtaccg gtcgaacaag gcacggcaac gcgtgtcgag ctttcgatcg
     4801 gctacagctt gaggggaatg ctggcgcaga tcgcccggga gggattagtc cgcgacctcg
     4861 ctgcccggct gaccgcggaa ttcgcccgca acctcgaacg gcaactgtcg ggcgccccgt
     4921 cggatggatc ggcggcggca gcgcaaaacc tgaatggtgt ttccttgctg atggggctgt
     4981 tgggcatgcg tgcgcgtaag gcgcttcggc gcgtttttgg ccgatagcga cagcgttact
     5041 ggtacgattc ttgctccgta cgcgccggga acagtcattt ttgtagggag ggcgtcatga
     5101 ccaattccga gctgcgtgcc gacactcaca gcatagaacc catccccgat gccgaccggg
     5161 actcgaccgg gccgcagcag atgtggatct gggccggggc gaacatcgcg ccggtcaact
     5221 gggcgctcgg cgcgctcggc atcattctca agctcggcct gtgggagacc atcggggtca
     5281 tcgtcgtggg caacctattc ggttgcgcca tcttcgccgc cttcacggtg atgggccaca
     5341 agaccggcgt caatcaaatg gtgctcagcc gctccgcgtt tgggcgccgc ggtgcctatc
     5401 tgccgagcgc gctgatgttc ctgatgacgc tgggctggat cggggtgaac acctacttcc
     5461 cggtgaagat ctcaatggcc attctgggcc agttcggaat cgccgacacg ttggtgacga
     5521 ccttcatcgt catcacgatc gtgatggtgc tgcaggtcgt catcggtatc tacggcttct
     5581 acgccatccg caccttcgag aagtatacgg taccgccgac cgtcgccatc atggtgctga
     5641 tgagcgttct ggcctggagc cagccggggg ttgtcaattg gcacctcgcc agcacgcttc
     5701 ctcctggcgc ccacctggcg atgttgacac tgctgatgtc ggcgatcggg gtgggctggg
     5761 gaatttcctg ggtcacatgg gcctcggact attcgcgctt cgtgccgcgc agcgtctcct
     5821 cgagctccgt gttctggtat agctatgtcg gaatgttcgt gccctcggta tggctcgcca
     5881 tcctcggcgc gaccatcgcc agcgttacac tggacaccga tccggccaag atggtcagcg
     5941 ccgtgttcgg cggcgtcgcg agcttgctgg tgctgctgat ggtgctgcac ggcccgatcg
     6001 cgaccaatat cctgaatgtg tattcggcgg cgctggctgc gctcagcatg ggcttgcgcc
     6061 tgtcgcggac ggcgctggca ctgatcgtcg gtatcgccgg ttatctggtc acggtctact
     6121 tcatcttcgc accctcgttc gccaaggcgt tcgacaactg gatgatcagt ctgctgctgt
     6181 ggatgagtcc ctgggcaggc gtcgtgatgg ccgatttctt tatcaagcgg aaaggccaga
     6241 tcgatgtcgc cgaactctac cgttcgccgg aaacaagcgc ctatggcgat atcaactggg
     6301 gcggcacggt cgcgttcttc gcgggactga tcgccggctg gtcagtgcag gacgggctcg
     6361 tcccggcgct gcaggggccg atctccaccg ggctgttggc tggagcggac ctcagctggc
     6421 tgttcgggat cgtcgtttcc ggagcggtgt atctggctct cagtcggcga gcggtggcaa
     6481 cgtcctccgc cgtggcaacc ggaacggccg cccagtaagg cggctgggac cagccacttg
     6541 cgaattttaa ccccagcaaa gggacccgat gttttataga tcgttgaaaa cctcaatcac
     6601 ggctggggcg gctatctgcc tgtcggttgc aaccgcacag caatcgaaag cggacgccgc
     6661 aagctgtcct tcgccgatcg gggcctttgg tgacttttgc aagaagtagg atcgcagtgc
     6721 gctggaacgc tcgcttcaac aacattttgg agcttgccga ggcgtgttcg gttccggtgc
     6781 ggtggtcatg ccgcacgggc gtatgccaca attgcgaaag cggcctgatc gacggccagt
     6841 tgcgctattc gccggagccc atcgacccgc cagccaaagg catcgccctg atctgctgtg
     6901 cgtcgccggt ttccgacatc gaactggacc tttgagcgcc gatcagagct gcatccgcaa
     6961 cggcggcggg ctccaagata tctagcgctt caatcacccc ttccgacctt gaatatcagg
     7021 gggactacgg ctttcggatg cacctttgat tggaatgacc ttggcggttt gttgagagcc
     7081 tgcgcggtcg tcggaatccg gatgctcatc ttgttgtgtg tacatcgggt acatcgcctc
     7141 cgcgtaggcg gcgcagccag cgagaatttc agcagccatc catgacgcga ttctgaacaa
     7201 gaggccgaat aagccaagac cggcgtttcg atggtgaccc gcgcctgacg caccacaagc
     7261 gatgtccaga tgtcgtgaat ccaacccggg cgacgatacg tcgccgttcg accgatgatt
     7321 ttcgatgggc attgatctct ccagctctca ggctggagaa tttttgccat gccaatgttg
     7381 ctgcactgtt ccggcatcgc aacgaaaggc tacggttgta accgggtaca atgaacagcg
     7441 cgagctaccg caccatttca gcgaagccaa aacgtgttac cgccgtaacg atgaacgtca
     7501 gccggcaacg cagccatcac gcgccgggcc tcggaatgac ggcgccatgc tcattgcggc
     7561 gcggttcgtt cgagcggatc cagaagtcgg gacaatcctg cgccgacgtc agtttctcgt
     7621 ggcggatcgg caccggcgcg tccacatgtg aaagcagcac aggtcgatgc gaacgacagc
     7681 gcgcggcgaa gctcgccgga attcgcctgc gccagcgatc cggttgcgat ccgtccgatg
     7741 gcacgcaaag caaacagcaa ggcagcctgg aaactgtcgc cggccccgat ggtatccgtc
     7801 acctctacag tgggcgcctc gacttcaacc tggcctgctt ccttatgcca tgcctgcgcc
     7861 cctcgaattc cacgcgtaac gacgaccaga cttgcacccg cctcaatcag cgagttggcc
     7921 gttcccgaat aatcgctacc gccatacaga aattcaaagt cgacatccga catccgcacg
     7981 atgtccgcga tggcggcaaa tgcattcatt cgctcgacat agcgggcctt gtgcctgacc
     8041 agcttgggcc tgcaattcgg atcgaaggaa atggtgaccg atccgcgtgt atcctcgagc
     8101 atggcgagcg cctgggctgc tccgctgtcg tcggcgagcg tggtcgaccc gacatgaacg
     8161 gcttcgatct gatcgaaggg gatggagccg tgccgataga tccagtttcg ggacgcggtc
     8221 ccctcgtcat agaaggcata ttgcggctcg ccggcgacgg tacgaacgaa cgcaagcgtc
     8281 gtctggtgct cgctccgcgt cgcatatcga agttcgacct ccgaggcgag ggcatggtct
     8341 gcaatcatgc ggccgaacag gtcggtcgaa atcccaccca caaatcctgc cggcgcgccg
     8401 agacgtgcca tgccgacagc gatgttgagg caggagcctc cagccacagg cacgagcgcg
     8461 tcgcgcccgt ccaccgactt gacgggcagg aaatcaatca aggcatctcc gcaactcagc
     8521 agcatcgttc taatttttcg tatcgacacc ggcgatcaat tcgattccgt gcatcgcgtc
     8581 gcggctttcc cgatccagtt ttcgcatcag tccatggacc tgtcgcttgg tgcggtggaa
     8641 ttcggtcatt cctggcgccg tcggttcgct caaacaaccg atcgccgaca tcgaggccat
     8701 tgcctccccg atcgacccat aagaactgcc ggccaccgca cccagcatgg cagctcccag
     8761 cagcaccggt tcctgcgttt ccggaaccgc caccgtcagg cccgtcgtgt ccgccatgat
     8821 ctggcggacc agcgaactgc gtcccgcacc gccgctgatc accatcaagt cgctgtcgac
     8881 accgtgcgac cggaatgtgt cgacgacatc cgcaaggcca taggcgagcc cgcatagtcc
     8941 cgccacgaac agccgctcca tcgagccgat gtcggcatcg agatccatcc ctgcgacgac
     9001 cgcgcgcgaa tcgggattgg caaacggcga acgattgccg agaaactccg gcagaacgtg
     9061 aatgtcgcgg gcaaaccatg ccgcctcgcc cgggttttgc gaacgcgcga caatgcgtcg
     9121 ctcgagaaat tccagaactt ccatgccgcc ggcatgcgcc gtcgcggcgg cctcgttata
     9181 ggcagggtgg gacctgacga gatgatcgat ggccgcgccc gccgcggact gaccgccttc
     9241 gttgagccag aatcccggaa ccattccgga ataataggga ccccaaacgc ccggaacgaa
     9301 gcaaggctcg gacgttgtcg ccatgatgca ggccgaggtt cccatgatgt aggcaagacg
     9361 acggcgtaca tcgaccggct gacccgaccg cgcgcgccct ccgatcgtgc ccacacctcc
     9421 ggcgtgagcg tcgatcagcg acgcgccgac gggcgtcccc tccagcaggc cgaagtcgcg
     9481 cgcggcagat tcggtcaggc cggtgcccag cggcgtgccg ggtgcgacga tctgctttcc
     9541 gattcttgcg tagctgtccg acgcgatatc tccaagaccg atgcgctcga aatagctgcc
     9601 gctccagcgc tgttcgtggg cgaggaaatt ccatttgcag gcgagcgtgc atatcgaacg
     9661 tagcgtcgat ccggtggccc gaaacgaaag ataatcggcg agatcgaaaa aatgaccggc
     9721 cctgttgtag gtcgacggca gatgccgctt cagccaaaga agttttggaa tttccatctc
     9781 gggcgagatc gagccgccga catagcggag aacgtcgtca tgcgtattgt tgacgaaacg
     9841 ggcttcagcg gtcgcccgat gatccatcca gacgatgata ttccgtcgtt cgtcgcccga
     9901 tgcgctgacc gtcaggggat tggcgccggc gtccagcacc acgagcgagc acgtcgcatc
     9961 gaaacccagg cccttgacgg cggaaggcgg aagggcggct tccgccatgg ccgcccgaac
    10021 agaagcggca cacgccgccc agatctcgga tgaagactgt tcgacaacgc tccccgcctc
    10081 gtgccagacc gtgatcggat gccgggccgt cgccagcagc tttcccgtct cgtcgaacac
    10141 tccagcgcgc gcgctcgatg ttccgacgtc gatgccgata aaagcctgcc tcattgcaac
    10201 cccgttcaag atcagcgcaa gctcaccagc acgtatagcc tccgtcgacg agaacgacgc
    10261 tgccggtcat caagcttgcg gcgtcagacg ccagaaacag gacgacggag gcaatttcgt
    10321 cgacctcccc catccgggcc atcggcgtgc cgccgatcca ggcatcatac atctgcgggt
    10381 tgttcttgac gaatgcgttg aggggggtgg taacgtaggt tggcgccacc gcgttgacgc
    10441 gcacgccgcg gcccccccac tcggctgcga gcgacttggt gagatggtgc actgcggcct
    10501 tcgaggcgtt gtagtaggac tgctcctgcg gtttgttgac gataaagccg gacatcgagc
    10561 cgatgttcac aatggaacct gactttgcat gcagcatatg cttgccaaag gcacggcaac
    10621 accagaacgt gccgttgaga ttgacgtcga cgacgttcag ccaatgctca tcggttacct
    10681 tttccgcggg ggtctcgctc cgcgcgatgc ccgcattgtt gacgagaatg tcgatctttc
    10741 cgtaccttga cgccaattga tccgcgacct cggagacgcg cgcggaatcc gtcacatcca
    10801 tagtgacgat ctcggcattc aagccttttg ccttcaggtt cgcacggccg gcatcggcag
    10861 cgccagggtc gcgatccgcg atcacgacca tcgctcccgc ctctgccagc gcttccgcgc
    10921 acgcaagtcc gattccctga ccggcacccg tcaccacggc cgtttttcgg tccagcttga
    10981 atttttccag atacatcttc tttgttccta cttacaggtc ggccatcggc aaagtcactt
    11041 cgccggtatc gtttgcgttt cgttcatgcc cgaatgaccc ctccgtcctt gtcgaagcgg
    11101 tgcaggcggg ccggatccgg cgacagccac acgcggtcgc cggctttcag gctgaactct
    11161 ccgatggcgc gcaccgtgag cgttcccagt ttgccggcgt cgacatagag gaaggtgtcg
    11221 ctgcccagat gctccgcgac cgacacggtc ccgggccagc cctccccctc tttgccgatt
    11281 ttcagatgtt cgggcctaat gcccaggatg gcgatgcccg gctcgccagc cgcctctccg
    11341 gaaacgaagt tcatcttggg cgatccgatg aaaccggcaa cgaacaggtt tgccggctgc
    11401 tcgtataatt cgagcggcga gccgtactgt tcgatgcggc ccgcattgag cacgacgatc
    11461 ttgtcggcca tcgtcatagc ctcgacctga tcgtgcgtga catagacggc ggtggttccg
    11521 agctgctttt gcagcttggt gacttccagg cgcatctgga cgcggagcgc ggcgtcgaga
    11581 ttcgacaaag gctcatcgaa tagaaacgcc ttcggctcgc gcacgatagc ccgcccgatc
    11641 gcgacgcgtt gacgctgccc gccggagaga tcgcgcggct tgcgatcgag ataaggcgtg
    11701 agattcagcg tggcggcggc ggcctccacc ttgcggtcga tctcctgacg cggcagccca
    11761 gccatcttca ggccgaacgc aatgttgcca cgaacgctca tatgcggata gagcgcataa
    11821 gactgaaaca ccatcgacag accgcgcttc gccggcggca cgtccaccac attgcggccg
    11881 tcgatcagga tctttccgcc ggtgacgtcc tctagtccgg caatcaaacg caacagcgtg
    11941 gtcttgccgc aacctgacgg tccgacgaaa accacgaagg cgccgtcctc gatgtccaga
    12001 tttgcatcct tgatgatgtt gaccggcccg aacgacttgc ggactccctg aagcgtgatc
    12061 tggcccatga tgtctagccc tttcgctgat ctatttgacg gcgccgaacg tcagtccgcg
    12121 gacgagctgc cgctgggtga accatccgag gacgatgatc ggcgctatgg caagcgtcga
    12181 ggccgccgac agctttgccc agaacaggcc ctcaggactt gaataggacg cgataaaaac
    12241 cgtgagcggc gctgcgtcca atgtcgaaag gttcagggtc caaaatgcct cgttccacgc
    12301 cagtatgaag ttgagaagaa gcgtcgaggc gagcccggga atggccatcg gcgccagcac
    12361 gtaaacaagc tcgcgtccga tagtcgctcc atccatccgc gccgcttcga ggatgtcctt
    12421 cgggatttcc ttgaaatagg tgaacagcat ccagatgacg atcggcaggt tgccgaggca
    12481 caggatcatg acgagaccgg cacgcgtatc gagcatccca aaatcgcggt agatcagata
    12541 aatcggaacc agcacgccga cgggcggcat catcttggtc gagagcatcc acagcagaac
    12601 gtccttggtg cgctttgttg gcgcaaacgc catcgaccag gccgccggca ccgcgatcag
    12661 catggcgatc agcgtcgatc cgccggcgat gaggatggaa ttg
//
LOCUS       DASWGM010000910         4376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1333961,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000910 DASWGM010000000
VERSION     DASWGM010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333961"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VGA15_24710"
     CDS             complement(<1..602)
                     /locus_tag="VGA15_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9590929.1"
                     /translation="MGRGFRIKMHVRRFTRRHPWLTFTVRSFMIFSAAFGGAYGFIAG
                     SRSEDSGYDPHAFAIGASFLFAVACVALATLSMRLRWMHRKLRKIALHNEALADRNWE
                     LQEAEQRSRSLFESQGDLIVLRGAGGCITFVNDAYCELAQLPRRALVGSDFTLDVLEQ
                     GDPALEQNGTRIHDQKIATALGPRWIAWREGLVRIDAGRPA"
     gene            complement(785..1252)
                     /locus_tag="VGA15_24715"
     CDS             complement(785..1252)
                     /locus_tag="VGA15_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590930.1"
                     /translation="MLRFKILASAVPLIVAAVFARGEFSISPRPCIAIGGASVQITAA
                     PWQAQLQVSFTSDPSIATVRVQISETAGAADFAVVDDVDSAEDGACAANAATRFVAIS
                     ANPQRSAPVIYLSPDGPADYRIFVHSKRFTARDAAALIVGASGVPSRLAAASL"
     gene            complement(1563..3011)
                     /locus_tag="VGA15_24720"
                     /pseudo
     CDS             complement(1563..3011)
                     /locus_tag="VGA15_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(3721..>4376)
                     /locus_tag="VGA15_24725"
     CDS             complement(3721..>4376)
                     /locus_tag="VGA15_24725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590931.1"
                     /translation="ARGEKRRLDPETVDAAKKIMERQGVNNPGMAENHPEIVEPWTTL
                     IVDLSAETLADAKAIQDRLADLLYPETLEDVEALKSLRSEGLKKISLGEKPNPEHLDA
                     AKALNEIRARDMWQRREEEEAEQRERVRERKQKEEQARLVDEMMKSSARNIARVHGLP
                     ENAFDGMRPEEVLMHARFLRLSQKFAHLPAEDREARTFEALERELNHEREERRRRTE"
BASE COUNT          849 a         1340 c         1353 g          834 t
ORIGIN      
        1 gccggccggc cggcatcgat acggaccagg ccttcgcgcc atgcgatcca tcgcggcccg
       61 agcgcggtcg cgattttctg gtcgtggatc cgcgtaccgt tctgttccag tgccggatcg
      121 ccttgctcaa gaacatcgag cgtgaaatcg ctgccgacca gcgcgcggcg cggcagctgc
      181 gccaattcgc aataggcgtc gttgacgaaa gtgatgcaac ctccggcacc gcgcagaaca
      241 atcaggtcgc cttgcgattc gaacaggctg cgggagcgct gctcggcttc ctgcaattcc
      301 cagttacggt cggccagcgc ttcgttatgc agagcgatct tgcgcagctt cctgtgcatc
      361 cagcgcagcc gcatgctcaa ggtcgcaagc gcaacacaag ccaccgcgaa caggaagctg
      421 gcgccgatcg cgaaggcatg ggggtcatag ccggaatctt cggaccggct gccggcaatg
      481 aatccatacg caccgccaaa ggcggcggag aagatcatga acgagcggac ggtgaaagtg
      541 agccagggat gacgccgcgt gaagcggcgc acgtgcatct tgatccggaa accccgcccc
      601 atagccaact cccctgagat tgccttctgg cagcgtgatc gcctagcgcg atcggcattg
      661 agactgcccc gacgcgcgcc atgtgctgcc gaagaccatg cggcgccgat cttgaaaaag
      721 tacttgagtc ggtgcgacgt ctcgccgttc gatgacaaca tggttgataa agcgttaagg
      781 cgcctcagag cgaggcggcg gcgaggcggc tcggtacgcc actggcgccg acgataagag
      841 ctgccgcatc gcgtgccgta aaacgcttcg aatgcacaaa aatccggtaa tcggccggac
      901 cgtccggcga gagatagatg actggcgcgg atcgctgcgg gtttgcggag atcgccacaa
      961 accgggtcgc tgcattggcg gcgcaggcgc cgtcttccgc actatcgaca tcatcgacta
     1021 cggcgaaatc ggccgcaccg gcggtctccg agatctgaac ccgcacggtg gcgatcgacg
     1081 ggtcgctggt gaaactgacc tgaagctgcg cttgccaggg tgcggctgtg atctggaccg
     1141 atgcgccgcc gatcgcgatg caggggcgtg ggctgatcga gaactcgccg cgcgcgaaca
     1201 cagcggcaac gatcaacggc acagccgaag caagaatctt gaaacgcaac atgatacgcc
     1261 cgcccgcaga taagcccgag aaaacgccgg gggcttatgg ttggcggaat gtaaacaaaa
     1321 ctcgttaccg gcgcgttcac gtgggagccc ggcgcatgaa actaccgatt gattttaaag
     1381 cgggatggct tggagttgaa ttggcgcgct catctcaaat ccgtcaccct gaggtgcgag
     1441 ctcttgcgag cctcgaagga tgacgtgcga cggtgtgtgc ggctcatcct tcgaggccgt
     1501 cgcaagcgcg acggcgcctc aggatgacgg gaatggtttg agtgccggtt cgatccaatg
     1561 cttcacgcag cacttctcag atcttcagcg agcgcgcggt aggacaaggc ttcggccaga
     1621 tgcagccggc cgatcttgtc ggcgccatcg agatcggcca gcgtgcgggc gacgcgcagc
     1681 acgcgatgat agccccgtgc cgacagccgc atggtctcgg cggcgtcgcg cagcagtttt
     1741 aacccatgcg cgtccggctt ggcgatttct tccagcaccg acgccggcgc ttccgcattg
     1801 gtgcggacat gcggcagtcc ggccgcggcg taacgcgcca attgaacgtc gcgcgccgcg
     1861 gcgacgcgcg cggcgacttc ggcggatcct tcggtcggcg gcggcaggat cagatcggcg
     1921 gcggtcacgg ccggcacttc gatccgcaag tcgatgcgat ccatcagcgg accggagatg
     1981 cgtgcctggt aatcgccggt gcagcggtcg atccgcccgc gcttgcagga atagccgggt
     2041 tcataggcat ggccgcagcg gcaagggttc atcgccgcga tgagcatgaa gcgggccgga
     2101 taggtgacgc gatgattggc gcgcgacacc gcgacctcgc cgttctccaa gggctggcgc
     2161 agcgaatcca gcacccgggg atcgaactcc ggcaattcgt cgagaaacag cacgccctga
     2221 tgcgcgagcg agatctcgcc cggcttggcg cgcatgccgc cgccggtcag cgccgccatg
     2281 ctggcggaat gatgggggct gcggaacggc cgccgcgccg tcaaggcgcc gtcgcggatt
     2341 tcgccggcga ccgaggcaat catcgaaact tcgagcagtt cggacggcga cagcggcggc
     2401 aggatcgaag gcagccgcgc cgcgagcatc gacttgccgg cgccgggaga gccgatcatc
     2461 agcagatgat gtccgccggc cgcggcaatc tcgagcgcgc gcttggcgct ttcctgtccc
     2521 ttgatgtcgc gcaggtcgag caggttggct ttcgcctcgt ggaccttcgg tgtggggcgc
     2581 gacagcacct gggtgccctt aaaatggttg gcgatctgga tcagcgaatg ggcggcgacg
     2641 atctggatat ccggacttgc ccatgccgct tccgccccgc acgccgcggg acagatcagg
     2701 ccttcgtcgc gcgaattggc gccgatcgcc gccggcaaca cccccgcgac cggcgcgatc
     2761 gatccgtcga ggccgagttc gccaagcacc gtgaagccgc tcaacgcgtc cggcggtatc
     2821 gcgccgatcg ccgccatcag gccgagcgcg atcggcagat cgtagtggct gccctccttc
     2881 ggcaggtcgg cgggtgcgag attgacggtg attcgccgcg ccggcagcgc gaggccggag
     2941 gcgatcagtg ccgagcggac ccgctcgcgg gcctcggaga ccgccttgtc gggcagcccg
     3001 acaatggcga atgtggatcg tcaatgacaa aattcgcgtg ggaatttggg tcccctttgg
     3061 tcgaccggag cctatttttg aacgcgatgg cgttccgact tcaaaattcg aagttaaagg
     3121 ccgccgccgc tgcccggctc gcagcagcag tgtgcgcgaa cctcggggtg cctgcctctt
     3181 gagcctccat gtggctccta agccaaggtt cagtcggttg accgacggat gcgccttgga
     3241 atgcggtgtc ggctttcgcc aactgcggac gtgccgtcgc acacgtccgg ggcagctatg
     3301 ggccaatagg agacgaacgg cactgcagaa aaaaatacct cacgaacctt ttgcccgcat
     3361 ccattctagc cgtacaaaaa ctaaatgtgc tgtaaggatg atcgcgacac caatatcgaa
     3421 gacgtgccaa gtcgtccgtg aaagatagat cgggattatc ggattaaaca gcaccgcaac
     3481 acagccgagt attgctgacc acatccgtga aattgcactg tcctcccatc ctacgagggc
     3541 aaggaagcaa gcaaatccgg cgacggcaat tcttgtgact gtatagtagc cgtaaggcat
     3601 tcgtgcgatt gctaggaaaa gcagcgcgat tggagcgagc cacagccacc acggtataga
     3661 attaactcgc atggaaagtc atcgcggcct tggcaattca actgatgttg tcatttagta
     3721 ctactccgtg cgccttcttc tttcttcacg ctcatggttt agctctctct caagcgcttc
     3781 aaaggtacgt gcctcacggt cttccgccgg taagtgggca aatttctggg agaggcggag
     3841 aaaacgagca tgcatcaaca cttcttcggg tcgcatacca tcaaaagcat tttcgggaag
     3901 accgtgcact cgcgcgatgt tcctcgccga gcttttcatc atctcgtcca ccaatctggc
     3961 ttgttcctcc ttttgtttcc tttcccgaac cctttcgcgt tgttcggcct cttcttcctc
     4021 tctgcgttgc cacatgtcgc gtgctctgat ttcgttcaac gccttggcag cgtctaaatg
     4081 ttcagggttc ggcttttccc ccagagaaat cttcttcaac ccctcgcttc gtaggctctt
     4141 caacgcttcc acgtcctcaa gcgtttcagg atagagaagg tcggcgagcc gatcctgtat
     4201 tgcctttgcg tccgctaggg tttcagcact caagtcaacg atgagcgtag tccatggctc
     4261 gacaatttca ggatggttct cagccattcc gggattattc accccttgcc gctccattat
     4321 tttcttcgcg gcatccacgg tttcgggatc aaggcgtcgc ttttcgccgc gcgcga
//
LOCUS       DASWGM010000911         6437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_126676,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000911 DASWGM010000000
VERSION     DASWGM010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126676"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1263)
                     /locus_tag="VGA15_24730"
                     /pseudo
     CDS             complement(74..1263)
                     /locus_tag="VGA15_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
     gene            1408..1923
                     /locus_tag="VGA15_24735"
     CDS             1408..1923
                     /locus_tag="VGA15_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9590932.1"
                     /translation="MNQRSKRAVETAVSPDKDERLAPITAMMSSRLMVLANLLKRGAI
                     LRYKRLAGLSSVEFGLVASLGRRPPMSVARLAEAVGTDKGQISRALAGLVSRKLVSRA
                     VNPQDNREVLVCLTRSGLIVHETIVAGALERNQRLLEQLGKDEVAALLEHIDRLTETA
                     AKMLDAEKDLS"
     gene            2101..2466
                     /locus_tag="VGA15_24740"
     CDS             2101..2466
                     /locus_tag="VGA15_24740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX9590933.1"
                     /translation="MTGILQNYLPLVVFIGVAGLIGLALLIAPFLVAFQQPDPEKLSA
                     YECGFNAFDDARMKFDVRFYLVAILFIIFDLEVAFLFPWAVAFGKLGATGFWSMLVFL
                     AVLTIGFAYEWKKGALEWD"
     gene            2457..3053
                     /locus_tag="VGA15_24745"
     CDS             2457..3053
                     /locus_tag="VGA15_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX9590934.1"
                     /translation="MGLSPALTQPGFAPAVAPAATGILDPSTGRPVGANDPYFLEVNH
                     ELSDKGFFVTATDDLITWARTGSLMWMTFGLACCAVEMMQMSMPRYDAERFGFAPRAS
                     PRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCD
                     RIVPIDIYVPGCPPTAEALLYGVMLLQKKIRRTGTIER"
     gene            3059..3388
                     /locus_tag="VGA15_24750"
     CDS             3059..3388
                     /locus_tag="VGA15_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HEX9590935.1"
                     /translation="MQINDIPFGTTDWSSVETTTHMGDSGVATWRTCQFGDIRVRLVE
                     YSPGYLADHWCQKGHILFCVDGELGTDLADGRSFTLTPGMSYQVADGAEPHRSHTAKG
                     ARLFIVD"
     gene            3398..4012
                     /locus_tag="VGA15_24755"
     CDS             3398..4012
                     /locus_tag="VGA15_24755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9590936.1"
                     /translation="MSEALTTLGEKIVAGLGGAATGHSIAFDQLTVAVQADKIVDVVK
                     FLRDDPGCRFVSIIDVTAVDYPGREKRFDVVYHLLSPTLNARIRLRTEADETTQVPSI
                     IEVFPGADWFERECYDLYGVIFTGHPDMRRLLTDYGFDGHPLRKDFPLTGFVEVRYDD
                     QEKRVVYEPVRLNQEFRKFDFLSPWEGADYPLPGDEKATDKAGS"
     gene            4012..5205
                     /locus_tag="VGA15_24760"
     CDS             4012..5205
                     /locus_tag="VGA15_24760"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX9590937.1"
                     /translation="MAETAALRNFTINFGPQHPAAHGVLRLVLELDGEVVARVDPHIG
                     LLHRGTEKLIEHKTYLQAIPYFDRLDYVAPMNQEHAFCLAAEKLLGIAVPRRGQLIRV
                     LYCEIGRILSHLLNVTTQAMDVGALTPPLWGFEEREKLMVFYERASGSRMHAAYFRIG
                     GVHQDLPPKLIDDIEAWCDPFLGVVADLERLLTGNRIFKQRNVDIGVVSLKQGWEWGF
                     SGVMVRGSGAAWDLRKAQPYECYAEMDFDIPIGKNGDCYDRYCIRMEEMRQSVRIMKQ
                     CIEKLRAADGQGPVAVEDNKIFPPRRGEMKRSMEALIHHFKLYTEGFHVPAGEVYAAV
                     EAPKGEFGVYLVADGSNKPYKCKIRAPGFAHLQAMDFICRGHLLADVSAILGSLDIVF
                     GEVDR"
     gene            5205..6167
                     /locus_tag="VGA15_24765"
     CDS             5205..6167
                     /locus_tag="VGA15_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9590938.1"
                     /translation="MAMPPIQFDRATGVLEGANAWERVAALALSSGSKVASTFSHRGY
                     NSCANLLRKTLPERDIAIKLNSDATFEFPYGDGYWSKLLNRSFSYEDELELLFQDSTD
                     VDYTLIDCGANFGYWSVLISSAPYGSHKAIAIEPSSQNFSKLANNAEINGNRFEVMKC
                     AIGSSRGTARLSGTKHEAFTIAGNVAGGGEDVPVIALDNLLDDGKISADGKYLIKLDV
                     EGVEIEAIKGGARLLQGDSVLLCEEHGNDPLHTVSRYILEQTPLKLIVYDPRTNRLET
                     VTELSILNRIKVSSHVGYNVFGTASAFWESRISALNANAARHMQ"
     gene            6182..>6437
                     /locus_tag="VGA15_24770"
     CDS             6182..>6437
                     /locus_tag="VGA15_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX9590939.1"
                     /translation="MAVRRLAPKELQPANFTFTAENLAWAQKEIAKYPEGRQTSAVIP
                     ILWRVQEQHEGWVSEAAIRAVADLLDMPYIRALEIATFYTM"
BASE COUNT         1184 a         1954 c         2033 g         1266 t
ORIGIN      
        1 cacggccacc tgcggcgcga aatcgaccat ggtatgcccc ccgagattct tttttagaac
       61 gaggcgccgc cgatcacgcc tcgcgaatga agttgccggc ctcgaactcg accggtggcg
      121 catccgggtc gaacgagacg cccgcaggat cgcgcccccc tgcgatagcc tcgagctgct
      181 cgctcagcat ccgccggatc atcaggatgc cgcggtcgct ctgaccaaag tgctcctcgg
      241 aatgcagcgt caccggcccc tggccgacct gcgcctcgta atcgccgggg aattgctggt
      301 gttcctgctc ggtcatgtcc caccagaact tgccgttgaa cttcgagcgc attcgtccga
      361 tatcgccgcg ctgcttgacc cggccggcga cgtagatacg gaacgaggtg tcatcgatcg
      421 gcagcgtcca gccgatcgac tcgacaggag cgaattgcgc gacccgtgga ttgggcacca
      481 cgcgcagggt aggcaggaca gcctcggtca cgcgataaaa aatcttgccg tcgtcctggt
      541 tgcggatcga gcgcacggtg acgccgcgtt gagacatctc gaatttcacc tcgggcatcg
      601 aggccatcat gttggtaaac tgaggaccgc taaacgagcc gtgcagtacc ggcacgtggt
      661 aggggtcgac cacgttctcg aagtgctgca gccagttgca gggaatgatg gtcggcccgc
      721 cgccgccgat cgaggtgtcg tcggcctcga cgaattcgcc gtcgtccatc ttttccagag
      781 cttggtagcg cggcagctcg ggcttttctc tgatggtccc atataggcga agatcagccc
      841 gtagcgttcg gcaaccgggt accagggctg gcgcatcttg tccctgaact ggccgccctc
      901 gggttcgcag ggctgctcaa ggcaacgccc ttcggtgtcg aacttccagc cgtgatagca
      961 gcagcggatg ccatcctcct cgaccttgcc ataatacagc gtggtgccgc ggtggcagca
     1021 gcgggcgtgg agcaggccga cgcggccatg ccggtcgcgg aacaggatca ggtcctcggc
     1081 cagtgcgcgg acctttcggg ggatgtcggt ggcgtcagcc acaaggccga tcgggtgcca
     1141 gtaacgccgc agcagttccc ccatcggggt gccgcgcccg acctcggtca gttcggtccg
     1201 gctggcggac ggcctcatgg cataggcggt gccgagatcg cgatctcgct gggtgatgtt
     1261 catcgccgct tcctccaatc cacagccttg aatccacagc cttggcggcc gttgttttag
     1321 cgttggcaaa gtgaacttta gatcgatgac aatgtcaacg gttcggaaaa ccttggcatc
     1381 agggggtttt attggcacac gtcagacatg aaccagaggt cgaagagggc ggtcgagacc
     1441 gcggtcagcc ccgacaagga tgaacgactt gcgcccatta cggcgatgat gtcgtcgcgg
     1501 ttgatggtgc tcgccaatct cctcaagcgc ggcgccattt tgcgctacaa acgtctggcc
     1561 gggctttctt cggtggagtt cgggctggtg gcgtcgctgg gtcgtcgacc gccgatgagc
     1621 gtcgccaggc tcgccgaggc ggtcggcacg gacaagggac agatcagccg ggcgttggcc
     1681 gggctggtgt cccgcaagct ggtttcgcga gcggtcaatc cacaggataa ccgggaagtg
     1741 ctggtctgcc ttacccggag cggactgatc gtccatgaga cgattgtggc gggtgcattg
     1801 gagcgcaatc agcgcttgct ggaacaactc ggcaaggacg aagtggcggc gttgctggag
     1861 catatcgatc gcctgaccga gaccgccgcc aaaatgctcg acgccgaaaa agacctgagc
     1921 tgagaaaagc tccgtgcctc aacttcgggc ggccttcccg ggttatcccg ccgctcgtgg
     1981 ggccgggcag ggccgtcaaa atagcttgtc ttgcgcccct tgttgcgctg cgctaagcct
     2041 cggaataatt ccaatgtaac gaatctgcgg cggtcgaaac cgccggaaaa ggccccgccg
     2101 atgaccggca tcctgcagaa ttaccttcca cttgtggtgt ttatcggggt cgccggcctg
     2161 atcggtctgg cgctcttgat cgctcccttc ctcgtcgcct tccagcagcc cgatcctgaa
     2221 aagctgtccg cttatgaatg cggattcaac gcttttgacg acgcccgcat gaagttcgac
     2281 gtccggtttt acctggtggc gatcctcttc atcatcttcg accttgaagt ggcgttcctg
     2341 ttcccgtggg cggtggcgtt cgggaagtta ggcgcgaccg gcttctggtc gatgctggtt
     2401 ttcctcgccg tgctcacgat cggcttcgcc tatgaatgga agaaaggcgc gcttgaatgg
     2461 gattgagccc cgcactcacg cagccaggat tcgcaccggc agtagcacct gccgcgaccg
     2521 gcattctcga tccctctacc ggccggccgg tcggcgccaa tgacccttac tttctcgagg
     2581 tcaatcacga gctgtccgac aagggcttct tcgttaccgc gaccgacgac ctgattacct
     2641 gggcgcgcac gggatcgctg atgtggatga ccttcggcct cgcatgctgc gccgttgaaa
     2701 tgatgcagat gtcgatgccg cgctacgacg ccgagcgttt tggattcgcg ccgcgcgctt
     2761 ccccgcggca gtccgacgtg atgatcgtcg cgggcacgct gaccaacaag atggcgccgg
     2821 cgctgcgcaa ggtctacgac cagatgccgg agccgcgtta cgtgatctcg atggggtcat
     2881 gcgccaacgg cggcggctac tatcattatt cgtattcggt ggtgcgcggc tgcgaccgca
     2941 tcgtgcccat cgacatctat gtcccgggct gcccaccaac ggcggaagcg ttgctctatg
     3001 gcgtgatgct gctgcagaag aagatcaggc gcaccggaac catcgaacgg tgatggcgat
     3061 gcagatcaat gacatcccgt tcggaaccac tgactggtcg agcgttgaga cgaccacgca
     3121 tatgggcgac tccggcgttg cgacctggcg cacctgccag ttcggcgaca tccgggtgcg
     3181 gctggtggaa tactcgccgg gctatctggc ggaccactgg tgccagaagg gtcatatcct
     3241 gttttgcgtc gatggtgagc ttgggaccga tctcgccgat ggccgctcgt tcacgctgac
     3301 gcccggcatg agctaccagg tcgccgacgg cgccgaaccg catcgctcgc acaccgcgaa
     3361 gggcgcgcgg ctgtttattg tggattgatg gatggcgatc agcgaagcac tgaccactct
     3421 cggagagaag atcgtcgccg gtttgggtgg ggccgcgacc ggccattcga tcgcgttcga
     3481 tcagctcacg gtcgccgtgc aggccgacaa aatcgtcgat gtcgtcaaat tcctgcgtga
     3541 cgatcctggc tgccgcttcg tcagcatcat cgacgtgacg gcggtggact atcccggccg
     3601 tgaaaagcgc ttcgacgtgg tctatcacct gctgtcgccg acgctgaatg cgcgcatccg
     3661 gttgcgcacg gaggccgatg agacgacaca agtgccttcg atcatcgagg tgtttcctgg
     3721 cgccgactgg ttcgaacgcg aatgctacga cctctacggc gtgatcttca ccggtcatcc
     3781 cgacatgcgg cggctgttga ccgactacgg tttcgacggc catccgctac gcaaggattt
     3841 cccgctcacc ggcttcgtcg aggtccgcta cgacgaccag gagaagcggg tggtctacga
     3901 acccgtccgt ctcaatcagg aattccgcaa attcgatttc ctctcgccat gggaaggcgc
     3961 tgattatccg ctgccgggcg atgagaaggc gaccgacaag gcgggcagct aatggccgaa
     4021 acagcagcac tccgcaactt tacgatcaac ttcgggccgc agcatccggc ggcgcacggc
     4081 gtgctgcgcc tggtgttgga gcttgacggt gaggttgtgg ccagggtcga tccgcatatt
     4141 ggcctgttgc atcgcggcac cgagaagctg atcgagcaca agacctatct gcaggcgatt
     4201 ccgtatttcg accggctcga ctatgtcgcg ccgatgaacc aggagcatgc gttctgtctg
     4261 gcggcggaaa aactgctcgg cattgccgtg ccgcgccgcg gccaactgat ccgggtcttg
     4321 tattgcgaga tcggccgcat cctgtcgcat ctgctcaacg tcaccacgca ggcgatggat
     4381 gtcggcgcgc tgaccccgcc gctgtggggc tttgaagagc gcgaaaagct gatggtgttt
     4441 tacgagcgcg cctccggctc gcggatgcac gcggcctatt tccggatcgg cggcgtgcat
     4501 caggacctgc cgccgaaact gatcgacgat atcgaggcct ggtgcgatcc gttcctgggc
     4561 gtggtcgccg atctcgagag attgctgacg ggcaaccgca ttttcaagca gcgcaacgtc
     4621 gacatcggcg tggtctcgct caagcagggg tgggagtggg gtttttccgg cgtgatggtg
     4681 cgcggctcgg gtgctgcgtg ggatctgcgc aaggcgcaac cttacgaatg ctacgccgag
     4741 atggatttcg acattccgat cggcaagaac ggcgactgct acgaccgcta ctgcatccgg
     4801 atggaagaga tgcgccagtc ggtgcgcatc atgaaacagt gcatcgagaa actgcgcgcc
     4861 gccgacgggc aggggccggt cgcggtcgag gacaacaaga tctttccgcc gcgccgcggc
     4921 gagatgaagc gctcgatgga ggcgctgatc catcacttca agctctacac cgaaggtttt
     4981 cacgtgccgg ccggcgaggt ctacgccgcc gtcgaggcgc cgaagggcga gttcggcgtc
     5041 tatctggtcg ccgacggcag caacaagccc tacaaatgca agattcgcgc gcccggtttt
     5101 gcgcatctgc aggcgatgga tttcatctgc aggggccatc tgttggccga cgtctcggcc
     5161 atcctcggtt cgctcgatat cgtgttcgga gaggtcgacc ggtgatggcg atgccgccca
     5221 tccagtttga ccgggctacc ggcgtgctcg agggcgccaa cgcgtgggag cgcgtcgcgg
     5281 cgctcgcgct gtcgtccggc tcgaaggtgg cgtcgacgtt ttcgcaccgc ggttacaaca
     5341 gctgcgccaa tctgctgcgc aagacgttgc ccgagcgcga cattgcgatc aagctcaact
     5401 ccgacgcgac gttcgagttt ccctatggcg acggctactg gagcaagctg ctcaaccggt
     5461 cattctccta cgaggacgaa ctcgagctgt tgttccagga ttccaccgac gtcgactaca
     5521 cgctgatcga ttgcggcgcc aacttcggct actggtcggt gctgatctcg agtgcgccct
     5581 acggttcgca caaggcgatc gcgatcgagc cgtcgtcgca gaacttttcc aagctcgcga
     5641 acaatgcgga gatcaatggc aaccgcttcg aggtcatgaa gtgcgcgatc ggttcgagca
     5701 ggggtaccgc gcggctctcc ggcaccaagc atgaggcttt caccatcgcc ggcaatgtgg
     5761 ctggtggcgg cgaggacgtg ccggtcatcg cgctcgataa tctgctcgac gacggcaaga
     5821 tttcggctga tggaaaatac ctgatcaagc tcgacgtcga gggcgtcgag atcgaagcca
     5881 tcaagggcgg cgcacggttg ttgcagggcg acagcgtctt gctgtgtgag gaacatggca
     5941 acgatcccct tcataccgtg tcgcgctata tcctcgaaca gacgccgctg aagctgatcg
     6001 tgtacgatcc gcgcaccaat cgcctggaaa ccgtgactga gctttcgata ctcaatcgca
     6061 tcaaggtttc atcccacgtc ggttataacg tgttcggcac cgcaagcgcg ttctgggaaa
     6121 gccggatcag cgccctgaac gcgaacgccg cgcgccacat gcaatgagag actgatcaac
     6181 aatggccgtc cgccgcctcg cgccgaagga actgcagccc gcgaacttca cgttcacagc
     6241 ggaaaatctt gcttgggcgc aaaaggagat cgcgaaatat ccggaaggcc gccagacgtc
     6301 cgcggtgatc ccgatcctgt ggcgggtcca ggaacagcat gagggatggg tttcggaagc
     6361 ggcgatccgc gcggtcgcgg atttgctgga catgccctac atccgcgcgc tggagatcgc
     6421 gaccttctac acgatgt
//
LOCUS       DASWGM010000912         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1334030,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000912 DASWGM010000000
VERSION     DASWGM010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334030"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VGA15_24775"
     CDS             complement(<1..525)
                     /locus_tag="VGA15_24775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590940.1"
                     /translation="MIDRKNRYGRTAARIHDADGLARRPSSVTIDIHAHIVVPQAAKL
                     AQPHLDISRIPLAYFADAATKEINAQQEKDVSEVMTTIDRRLFDLDKMGIDIQVVAPA
                     PGQCYYSIDPKIAETAHRLANEGVVDYCSRKPDRFVGLGVVTLQEPEIAVRELDYIMD
                     DLKLRGVEILTNVDG"
     gene            complement(568..1458)
                     /locus_tag="VGA15_24780"
     CDS             complement(568..1458)
                     /locus_tag="VGA15_24780"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590941.1"
                     /translation="MKVGVCGTGRMGSSIAQRLMSVGHEIGVWNRNSEKTKSLIDAGA
                     KPFASPAELADGCEVVVVMLLNDAATEAVYRGPNGILRSKLAGKLVIDMSTIRPDTMK
                     SNGASVLEQGAAFVECPVGGSTGPAKEGKLFGLVGGTKKDVTRALPLLEQMCRRIEHI
                     GELGSGSMMKLAVNLPLLVYWQALGEALTICKPLDLPADRLIDILSDTAGTPTAMKGR
                     GAIIAKVLGGAPLGETAFGLNAAKKDLATAVQFGTSIHVELPVTASALACYEQAEAAG
                     LGDADATAISTRWPQSTAKS"
     gene            complement(1512..2237)
                     /locus_tag="VGA15_24785"
     CDS             complement(1512..2237)
                     /locus_tag="VGA15_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590942.1"
                     /translation="MAPDALRLTNVHAFYGDSHILHGISFSLQPGGVLALLGRNGAGK
                     TTCISAIIGFLTPRSGEIRLFGEAIEGLSPERISHLGIGLVPQGRRIFPSLSVRENLI
                     VAQQREGTTENPWNVERIYALFPRLRERHAQYAGTLSGGEQQMLAIGRALMGNPRVLL
                     LDEPSEGLAPLIVAEVGRTIKRLKEEGQSIVLVEQNIQLALDVADQAVILNTGRCVFA
                     GTAGEILNSEELIAQNLGVVHTH"
     gene            complement(2227..2982)
                     /locus_tag="VGA15_24790"
     CDS             complement(2227..2982)
                     /locus_tag="VGA15_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590943.1"
                     /translation="MNPLTSDFALEIVNLNKAFGGLVVTQNVSLAMRPGERRLIIGPN
                     GAGKTTLFNQISGDMRPSSGTIKLFGTDVTQLAPYKRAHFGLSRTYQIITLFTGDTLE
                     HNVTLGLLGLLPLRWQMWRPLSFYGDLATESRRILDTVGLLHLATHPVSDIAYGEKRR
                     VELAMALAQKPRVLLLDEPLAGLSNTERSTVKSLISSIPRETTVVMIEHDMDTALDLA
                     ETVTLLNYGRVIVDGDRDAVIADERTREVYLGA"
     gene            complement(2979..>3285)
                     /locus_tag="VGA15_24795"
     CDS             complement(2979..>3285)
                     /locus_tag="VGA15_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9590944.1"
                     /translation="FWGGISGLLYVYYNKYIHPTSLSTTSSAEALLGVIAGGSGTLGG
                     PVVGAALVLLLKNYASAYIERWNMLLGLVFLFIVLVMPTGIVPGLSRLLVGLRKGPR"
BASE COUNT          676 a          974 c          958 g          677 t
ORIGIN      
        1 accatcgacg ttggtcagga tctcgacgcc cctcaacttc aagtcgtcca tgatgtaatc
       61 gagctcacgg accgcgattt ccggctcctg caacgtaacg acgccgagtc ccacgaaccg
      121 atctggcttg cgcgagcagt aatcgacgac gccctcattg gcgaggcgat gggcggtttc
      181 cgcgatcttg gggtcgatcg aatagtaaca ctggcccggc gccggcgcca cgacctgaat
      241 gtcgatgccc atcttgtcga gatcgaacaa acggcgatcg atcgtcgtca tgacttcgct
      301 gacgtccttc tcctgctgcg cgttgatttc tttggttgca gcgtctgcga aatatgcgag
      361 cggaatccgg ctgatatcga ggtgcggttg cgccagcttg gcggcctggg gaaccacgat
      421 gtgggcgtgg atatcgatcg tgacagacga cgggcggcgg gcgaggccat cggcatcgtg
      481 gatacgtgct gcggtgcggc catagcgatt cttgcgatcg atcattgaaa tgctcgtttg
      541 ctaaaggtga cggttcgggg cgggcgatta cgacttggcc gtgctttggg gccagcgggt
      601 cgaaatcgcg gtggcgtcgg catcgccaag gcctgccgct tcggcttgct catagcaggc
      661 cagcgcgctt gccgtaacgg gaagctcgac atgaattgac gtgccgaact gaaccgcggt
      721 ggccagatcc tttttcgccg cgttgaggcc gaacgcggtt tcgccgagcg gggctccgcc
      781 caataccttg gcgataatgg cgccgcgtcc tttcatggcg gttggggtgc cggcggtgtc
      841 ggacagaata tcgatcagcc ggtcggcggg aagatcgagc ggcttgcaga tagtcaaagc
      901 ctcgccaagc gcctgccagt aaaccagcag cggcagattg accgcaagct tcatcatcga
      961 gcccgaaccg agctctccga tatgttcgat gcggcggcac atttgctcaa gaagcggcag
     1021 tgccctggtg acatctttct tcgtaccccc gacaaggccg aatagcttgc cttccttcgc
     1081 cggaccggtg ctgccgccca ccggacactc gacaaaagca gcaccttgct ccaacacgga
     1141 agcaccgttc gatttcatcg tgtccggacg aatggtactc atatcgatca cgagcttgcc
     1201 ggcgagtttg gatctcagaa tgccgttcgg cccgcggtaa acggcctctg tcgccgcatc
     1261 attcagcagc atcacgacga cgacttcgca accgtcggcg agttctgcgg gggatgcgaa
     1321 cggctttgca ccggcatcga tcagggattt tgtcttctct gaattgcgat tccagactcc
     1381 gatttcatgt cctacggaca tcaatcgctg cgcgatcgat gatcccattc gtccagttcc
     1441 gcatacgccg actttcatgg cttgacctca ttgagagagt ggattcgtgg ttttctcatc
     1501 tcttgtttcg cctaatgagt atgaacgacg ccgagattct gcgcgatcag ctcttcgctg
     1561 ttcaggattt cgccggccgt gccggcgaac acacagcgtc cggtgttgag gatgaccgcc
     1621 tgatccgcaa cgtcgagcgc taactggata ttttgttcta caagaacgat ggattgtcct
     1681 tcctccttca gccgtttgat ggtgcgaccg acttcggcga cgatcagagg cgcaagacct
     1741 tccgacggct catcgagcag aagcacccgc ggattaccca tcagcgcgcg cccgatcgcg
     1801 agcatctgct gttcgcctcc agagagcgtt ccggcatatt gcgcgtggcg ctcccgcagc
     1861 cgcggaaaca gggcgtaaat ccgctcgaca ttccagggat tttcggtcgt gccctcgcgc
     1921 tgctgcgcca cgatgaggtt ttcccgaacc gacagagacg gaaagatgcg gcgcccctgc
     1981 ggtacaaggc cgattccgag atgcgagatg cgttcggggc tcagcccttc gattgcctca
     2041 ccaaacagcc tgatctcgcc gctccggggt gttagaaagc cgatgatcgc cgagatgcag
     2101 gtggtctttc cggcaccatt gcgtcccagc agcgccagca cgccgcccgg ctgcagtgaa
     2161 aagctgatgc catgcaatat atggctgtcg ccatagaatg catgcacatt tgtcaaacgg
     2221 agtgcatcag gcgccaagat acacctcacg ggttcgctca tccgcgatca cggcatcgcg
     2281 gtcgccatca acgataacgc ggccgtaatt cagcagcgtc acggtctctg caagatcaag
     2341 ggcggtatcc atgtcatgtt ctatcataac cacggtcgtc tcccgaggga tcgatgagat
     2401 cagcgattta acggtggatc gctcggtatt tgaaagaccc gccagcggtt cgtccagcag
     2461 cagcacgcgc ggcttttgcg caagcgccat ggcgagctca acccggcgct tttcgccata
     2521 ggcgatatcc gacaccggat gcgtcgccag atgcaacagt cccaccgtat cgaggattcg
     2581 ccgggactcg gtcgcaaggt cgccgtaaaa cgataacggc cgccacatct gccaacgcaa
     2641 cggcagcaaa ccgagcaggc cgagcgtaac gttgtgctcg agcgtatcgc cagtgaacag
     2701 cgtaatgatc tggtaggttc gcgacagccc aaaatgcgcg cgcttgtacg gcgcaagttg
     2761 cgtaacgtcg gttccgaata gtttgatggt tcccgaacta ggccgcatgt cgccgctgat
     2821 ttggttgaac agggtggtct tgccggcacc atttggacca atgatcagac ggcgttcgcc
     2881 tggccgcatc gcgagagaga cgttttgcgt tacgacaagg ccaccaaagg ccttgttcag
     2941 gttgacgatc tcaagcgcaa aatctgatgt cagcgggttc atcgcggccc tttgcgcagt
     3001 cccaccagca gcctgcttag gcccggaacg attccggtcg gcatcaccaa aacgatgaac
     3061 aggaagacca gacccagcag catgttccat cgctcgatat aggccgaggc gtagttcttc
     3121 agcaacagca ccagtgccgc gccaacgacc ggaccgccga gggttcccga acccccggca
     3181 ataacgccaa gcaaggcctc ggccgaactg gtggtagaaa gtgatgtggg gtggatatac
     3241 ttattataat agacgtaaag caatccggat atgccgcccc agaag
//
LOCUS       DASWGM010000913         4111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2196530,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000913 DASWGM010000000
VERSION     DASWGM010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196530"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1229
                     /locus_tag="VGA15_24800"
     CDS             30..1229
                     /locus_tag="VGA15_24800"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9590945.1"
                     /translation="MPGPLNGVRVLDLTGVVSGPFATMFLADQGADVLKIEPIGGDIT
                     RRSRATIDKAGEFSALFISSNRGKRSLSIDVKSAVGREVLAKLVAQADVLVQNFRPGT
                     MERLGLGADELRERHPRLIYVSISGVGDTGPYVKKRVYDPIIQGLSGFADIQSQPVTN
                     RPQMIRTIVCDKTTAVFTAQAVSSALYAREKTGKGDHIQVAMLDAMISYLWPEGMMQY
                     TVVGSEATSADPNDRPDLVFKTSDGYITAGTISDSEWQGFCRASGDPELARDPRFATP
                     SARSVNATARINKMAEYIAERTTAEWLERLDAADVPCAPILRRGEIIHNEQVVARGII
                     AEFDQPAVGRVRQPKPAARFSVNEAVIGGPAPRVGEHSRDVLRDLGYDDGAIDKMVSE
                     KSVRVAV"
     gene            complement(1235..1912)
                     /locus_tag="VGA15_24805"
     CDS             complement(1235..1912)
                     /locus_tag="VGA15_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9590946.1"
                     /translation="MGARPDLTLWGVGTSRTIRPHWAMHELGLSYKTKPIGPRTGETK
                     TAEYTKLNPRQKVPLLQDGDFCIGESAAIVAYLSRMYSTPERALIPETPREYAAWLEW
                     CFFIVAELDSTSLYVMRRHRADALGHIYGVAPDVVAQAGEYFRQQLRHVETAFADGRT
                     FLMGDRFTSADILLTTCLDWAIAYGVGICDNAHPYLERIQARPAYRLAKAANVPLAPI
                     VPVQQKT"
     gene            complement(1912..2652)
                     /locus_tag="VGA15_24810"
     CDS             complement(1912..2652)
                     /locus_tag="VGA15_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590947.1"
                     /translation="MEGRGVAILVGAGDAIGAAVARRFAKGGYTVCICRRDAAKSRGL
                     VDELNAAGHSAHAFNVDARQEAEVQKLFADVEKNIGPIEICLYNAGSNVNKPLLETTE
                     KLFFKAWELACYGGFLVGREAAKYMLARGRGTIFFTGATASIRGGNGFAAFSSAKFGL
                     RAVAQAMARELGPKNIHVVHLLIDAGVDSEAIHQRMKAARGIEASEIPPDSLTKTSSI
                     AEAYWFAHQQTRDGWTHELDLRPSVEKW"
     gene            2879..>4111
                     /locus_tag="VGA15_24815"
     CDS             2879..>4111
                     /locus_tag="VGA15_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX9590948.1"
                     /translation="MTKQSEAIDLAEIVAGLPSRIHQVADGHVAEFPDRAALIEDGSS
                     WSYRELDRSVKEIAAVLASLGVRPGDRMIIVSENCIALAALLLAASRIDAWAIVANPR
                     LSARELDQIRDHSGARRIFFTAAVSKEAVAHASRCEAEVRQLGPLKEIGVSALNEGTT
                     AELVEIDPAKQVAVLIYTSGTTGTPKGVMLTHENLLISAKTTAHFRKMDAGDKVYVVL
                     PISHIVGISLLIMTLMVGGTVRMASKYDPAALARAIAEEGITILNGVPATYRRLLEYK
                     AVTGLAQLDRGSLRLIAVAGAPLDLDLKSGVEREFGLPLSNGYGITECSPGISGVRFD
                     APRADEAVGALLPGIEARLVRLDGGMVPNGEIGELHVRGRNVMRGYYRAPELTAKAID
                     TDGWFNTGDLARFDGDCLY"
BASE COUNT          773 a         1253 c         1304 g          781 t
ORIGIN      
        1 gctgcggaac gtaactcaag gagaatgtca tgcccggtcc actcaacggc gttcgcgtgc
       61 tcgatttgac gggcgtggtc tcgggtccgt tcgcgaccat gtttctggcg gaccagggcg
      121 ccgacgtgct caagatcgag ccgatcggcg gcgacatcac ccgccgcagc cgcgccacca
      181 tcgacaaggc tggagagttc tccgcgctct tcatctcgtc aaaccgtggc aagcgctcgc
      241 tgtcgatcga cgtcaagagt gcggtcggcc gcgaggttct cgccaagctc gtcgcgcaag
      301 ctgacgtgct ggtgcagaat tttcgacccg gcaccatgga gcgccttggc ctcggcgccg
      361 atgaattgcg cgagcgccat ccgcgcctga tctatgtttc aatcagcggc gtcggcgata
      421 ccggacccta tgtgaaaaag cgggtctatg atccgatcat tcagggtctg tccggattcg
      481 ccgatatcca gtcgcagccg gtgaccaatc gtccgcagat gatccgcacc atcgtctgcg
      541 acaagaccac cgccgtcttt accgcccagg ccgtgtcgtc ggcgctgtac gcgcgcgaga
      601 aaaccggcaa aggcgaccat atccaggttg cgatgctcga tgccatgatc tcgtatctct
      661 ggccggaggg catgatgcag tacaccgtgg tcggatccga ggcgaccagc gccgatccga
      721 acgatcggcc ggatctcgtg ttcaagacca gcgacggcta catcacggcc ggcaccattt
      781 ccgattccga atggcagggc ttctgcaggg cgtccggcga tccggaactt gccagggatc
      841 cgcgctttgc gacgccgtcg gcgcgctcgg tcaacgccac cgcgcgcatc aacaagatgg
      901 ccgaatatat cgcagagcgt accaccgcgg aatggctgga gcggctcgac gccgccgacg
      961 ttccctgcgc gccgatcctg cgccgcggcg agatcatcca taacgagcag gtggtggccc
     1021 gcggtattat cgccgagttc gatcaacctg cagtgggacg ggtgcggcag ccgaagcccg
     1081 cggcgcggtt ctccgtcaac gaggctgtca tcggcggacc ggcgccccgc gtcggcgaac
     1141 attcccgcga tgtgctgcgc gatcttggtt acgacgacgg cgccatcgac aagatggtca
     1201 gcgaaaagtc ggtccgggta gcggtctaat ccggtcaggt cttttgctgc accggaacga
     1261 tcggcgccag cgggacgttc gccgccttgg cgagccgata ggccggtcgc gcctggatgc
     1321 gttcgagata gggatgcgcg ttgtcgcaaa tgccgacgcc gtaggcgatc gcccaatcca
     1381 ggcaggtcgt gagcaggatg tcggcgctgg tgaacctgtc gcccatcagg aacgtgcggc
     1441 cgtcggcgaa ggcggtttcc acatggcgca gttgttgacg gaagtattca ccggcctgcg
     1501 caaccacgtc gggcgcaaca ccatagatgt gtcccaacgc gtcggcgcga tggcggcgca
     1561 tcacgtaaag gcttgtggag tcgagctccg cgacaataaa gaagcaccat tccagccacg
     1621 ccgcatattc gcgcggtgtt tcaggaatca gcgcgcgttc cggtgtcgag tacattcgcg
     1681 agaggtaggc cactatcgcg gcactttcac caatgcagaa gtcgccgtcc tgcagcagag
     1741 ggaccttttg acggggattg agcttggtgt attcggcggt cttggtctcg ccggtccgcg
     1801 gtccgatcgg cttcgtcttg tacgacaagc ccagttcgtg catggcccag tggggacgaa
     1861 tggtgcggct cgttccaact ccccacagcg tcaggtccgg tcttgcgccc atcaccactt
     1921 ctccacggat ggtcggagat cgagttcatg ggtccagccg tcccgggttt gctgatgagc
     1981 gaaccaatag gcctcggcga tcgaagacgt tttcgtcagg ctgtcgggag ggatctcgct
     2041 ggcttcgatg cctctcgccg ccttcatgcg ctgatggatc gcttcgctgt ccacgcccgc
     2101 atcgatcagg agatgcacga cgtggatatt cttgggcccg agttcgcgcg ccatcgcctg
     2161 agccaccgcg cgcagtccga atttcgccga cgaaaatgcc gcgaagccgt tgccgccgcg
     2221 gatgctggcg gttgcgccgg tgaagaagat ggtgccgcgc ccgcgcgcaa gcatgtactt
     2281 tgcggcctcg cggccgacca ggaacccgcc gtaacaggca agctcccagg ccttgaagaa
     2341 cagcttctcg gtggtctcca gcaggggctt gttgacgttg gatcccgcat tgtagaggca
     2401 gatctcgatc ggtccgatgt tcttttcgac atcggcaaac agcttctgaa cctcagcttc
     2461 ctgacgggca tcgacgttga acgcgtgagc gctgtggccc gcggcattca gctcgtcgac
     2521 caatccccgg gacttggctg cgtcacgccg gcaaatgcag acggtataac cgcccttggc
     2581 aaaacgacgg gcgacggcgg cgccgatggc gtcgcccgcg ccgacaagta tcgctacgcc
     2641 gcgaccttcc atcatcgtcg ctctcccagt aagttcttat ttggtaccta accaataccg
     2701 gggcccggag taattgtaaa tgccaatcgc gggaaatgcc aattaagtta gctcccattg
     2761 cgattcttca ccaagggggc cgaccgagaa tagagattga cgagttctaa aaaagaacgt
     2821 taattgcagc ggcgataagg tcgcgtgaaa agcggcgcga tgcatcggga ggtgggccgt
     2881 gacgaaacag tcggaagcga tcgatctcgc cgagatcgtc gccgggcttc cgagccgcat
     2941 ccatcaagta gctgatgggc atgttgccga atttcccgac cgtgccgcgt tgatcgagga
     3001 cggcagctcc tggagctatc gcgaactgga tcggtcggtc aaagagattg ccgcggtgct
     3061 cgcctcgctc ggcgtcagac ccggcgatcg gatgatcatt gtcagcgaaa actgcattgc
     3121 gcttgccgcg ttgctgctgg cggcgagccg gatcgatgca tgggcgatcg tcgccaatcc
     3181 gagattgtcg gcgcgcgagc tggatcagat ccgcgatcac agcggcgcgc gccggatatt
     3241 ctttacggct gcagtctcaa aggaggcggt ggctcacgct tcacgctgtg aggccgaggt
     3301 ccgtcaactc ggcccgctaa aggagatcgg ggtcagtgcg ctgaacgaag gcacgacggc
     3361 cgagctggtt gaaatcgatc cagcgaagca ggtcgcggtg ctgatctaca cctcgggaac
     3421 gacaggtacg ccgaagggcg tgatgctcac ccatgaaaac ctgctcatca gcgcgaaaac
     3481 cacggcgcat ttccgaaaga tggatgccgg cgacaaggtg tatgtcgtgc tgccgatctc
     3541 gcatatcgtc ggcatctcgc tgctgatcat gaccctgatg gtcggcggga cggtccgcat
     3601 ggccagcaaa tatgacccag cggcactggc cagggctatc gccgaggagg gaatcacgat
     3661 cctgaacggc gtgcccgcca cttatcggcg gttgctggaa tacaaggccg tgaccggtct
     3721 tgcgcaactc gaccgcggat cgctgcgact gatcgcggtt gccggtgctc cgcttgatct
     3781 cgacctgaag tcaggcgttg aacgagagtt cggtctgcct ctttcaaacg gttacggcat
     3841 caccgagtgt tcgccgggaa tatccggcgt gcgcttcgac gcgccgcgcg ccgacgaggc
     3901 ggttggagcg ttgctgccgg gcatcgaggc tcgcctcgtc cggctcgatg gcggaatggt
     3961 gccgaacgga gaaatcgggg aacttcacgt ccgcggccgc aacgtgatgc gcggctatta
     4021 tcgcgccccc gagctcacgg caaaggccat cgatacggac ggttggttca acaccggtga
     4081 tctcgcgcgc ttcgacggcg attgcctcta t
//
LOCUS       DASWGM010000914         3823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1206216,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000914 DASWGM010000000
VERSION     DASWGM010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206216"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1354
                     /locus_tag="VGA15_24820"
     CDS             71..1354
                     /locus_tag="VGA15_24820"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX9590949.1"
                     /translation="MARPVFIIDGSRTPFLKARSGPGPFTPVDLAVQCGRPLLARQPF
                     APTAFDQVILGCVNVIADEMNPARVAALRLGMGEAMVAFTVQINCGSGMQSIDTAYRY
                     IREGVSDLILAGGSEALSYAPLVWPQQGVRWFAGLAGAKGILGKLAAIAKARPAYFKP
                     IIGLERGLTDPITELNMGQTAEVVGHLFGITRAQSDAYAVESHKRLANAQAQGFLKGE
                     VETAFARDGKFYDHDDGVRPDSSPESLAKLKPVFERPWGQVTAGNSSQITDGASWTIL
                     ASEEAVAKHGLTPKAVIVDSQWSALDPSIMGLGPVLSATELLKRNKLSLQDIETWELN
                     EAFATQVLGCLAAWNDEKFCREILGLEGAAGQIDQTKLNVDGGAISLGHPVGCSGNRI
                     VLHLVNAMKRLGTKRGIATECIGGGQGGAMLIETV"
     gene            1363..3459
                     /locus_tag="VGA15_24825"
     CDS             1363..3459
                     /locus_tag="VGA15_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX9590950.1"
                     /translation="MDSRIMDVLGDRVLELGPKVDETGPYRNFKLTHDGDGIAWLLFD
                     RAGASANTLAADVIEELDLVLAALESQRPSGIVIRSAKKSGFIAGADVNEFRGASDPG
                     PVETGIGRAHAVIDRLEALKIPTVAVIHGFCLGGGLEVALACKSRIAIDGARFGFPEV
                     MLGLHPGLGGTARFTRLVNPMQAMTLMLTGKTIDARRAKSLGLVDAVAQERHVRNAVK
                     DAVFGRLKPAKPGPLNAILNFTPVRGFLASRMRAEAEKAAPHEHYPAPYALIDLWEKH
                     GGDTPAMLAAEKTSFANLMVTPTAQNLIRVFFLREQMKKLAGSGNKIGHVHVIGAGAM
                     GGDIAAWCANQDMRVTLADMKPEPIAGAVKRAADLFGKIMRKRTDVRDALDRLMPDLD
                     GEGVRGADLIIEAVPEKLELKQKVYAGLEPKMKPGAILATNTSSIPLQDLRTTLDKPA
                     WLLGLHFFSPVSRLQLVEVVSHDGTDPQMLKEALAFVDAIDRLPLPVKSSPGFLVNRA
                     LTPYMLEAMVMLDEKIDKLTIDAAAEKFGMPMGPIELADQVGLDICLAVGDMLRSKFG
                     DALPPTPAWLREKVAAGDLGRKTGRGFYVWKDGKAEKMSGSPAIVQPSPEMIDRLILP
                     MSNVCVACLREGIVDNADMVDGAMIFGTGYAPFRGGPLNYARTRGPENVVSALRALVT
                     KFGGRFTPDAGWENFK"
     gene            3456..>3823
                     /locus_tag="VGA15_24830"
     CDS             3456..>3823
                     /locus_tag="VGA15_24830"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX9590951.1"
                     /translation="MTDTQAVHAPPTSETEPCGDLCTRTLAMPADTNQNGDIFGGWLL
                     SQMDIGGGIFASKMAKSRTVTVAIEAMNFRKPVFVGDLVSVHANLVRIGRTSVTVHLE
                     AWVLRRKEMHSILVTDGNFT"
BASE COUNT          719 a         1151 c         1255 g          698 t
ORIGIN      
        1 cgatttacaa gaagcccgcc gaagtgcatc agttcttgca aggtctgggt gagacaaggg
       61 ctgcgagctg atggcgcgac cggtttttat catcgacggc agccggacgc cgttcctgaa
      121 agcgcgcagc gggccgggcc catttacccc ggtcgatctc gccgtgcagt gcggccggcc
      181 cttgctggcg cggcagccat tcgcaccgac cgcgttcgat caggtcatcc tcggctgcgt
      241 caatgtcatc gccgatgaga tgaacccggc gcgggtcgca gcacttcggc tcggcatggg
      301 cgaggcgatg gtggcgttca cggtgcagat caattgcggc tccggcatgc agtcgatcga
      361 cacggcgtac cgctatatcc gcgagggtgt ttcggacttg atcctcgccg gtggttcgga
      421 agcgctgagt tacgcgccgc tggtgtggcc gcagcagggc gtgcgctggt tcgcaggcct
      481 cgccggcgcc aagggcattc tcggcaagtt agccgcgatc gccaaagcgc gtccggctta
      541 tttcaagccg atcatcgggc tggagcgcgg gctgaccgat ccgatcaccg aattgaatat
      601 gggccagacc gccgaagtgg tcggtcacct cttcggcatc acccgcgcgc agtcggatgc
      661 ctatgcggtc gaaagccata agcggctggc caatgcgcag gcgcagggtt tcctcaaggg
      721 cgaggtcgaa accgcattcg cgcgcgacgg caaattctac gatcacgacg acggggtgcg
      781 gccggattcc tcgccggaga gtctggcaaa actcaagccg gtgttcgagc gtccgtgggg
      841 ccaggtcacc gccggcaatt cctcgcagat caccgacggc gcgtcgtgga cgatcctggc
      901 gtctgaggaa gccgtcgcaa aacacgggct gacgccaaag gccgtgatcg tcgacagcca
      961 atggtcggcg ctcgatccga gcatcatggg cctcggcccg gtgctgtcgg cgaccgaact
     1021 gttgaagcgc aacaaattgt cactgcaaga catcgagacc tgggaattga acgaggcgtt
     1081 cgcgacgcag gtgctgggat gtctggccgc ctggaacgac gaaaagttct gccgcgagat
     1141 ccttgggctt gaaggcgccg ccgggcaaat tgaccagaca aaactgaatg tcgacggcgg
     1201 cgcgatcagc ctcggacatc ccgtgggctg cagcggcaat cgcatcgtgc tgcatctcgt
     1261 caacgcgatg aagcggctgg gcaccaaacg cggcatcgcc accgaatgta tcggcggcgg
     1321 gcagggcggc gccatgctga tcgagacggt atagggaacg tcatggactc aaggatcatg
     1381 gatgttctcg gcgaccgcgt gctcgaactc gggccgaagg tcgacgagac cggaccctat
     1441 cgcaatttca aattgaccca tgacggcgat ggcatcgcct ggcttttgtt cgatcgcgcc
     1501 ggtgccagcg ccaacacgct ggcggccgat gtcatcgaag agctggacct ggtgttggcg
     1561 gcgctggaaa gccagcggcc gtcgggaatt gtgatccgct ccgcgaaaaa atccggcttc
     1621 atcgccggcg ccgacgtcaa cgaatttcgc ggcgcgagcg atcccgggcc cgtcgaaacg
     1681 ggaatcggcc gcgcccatgc cgtgatcgac cgcctcgaag cgttaaagat tccgaccgtt
     1741 gcggtgatcc acggtttttg tctcggcggc ggcctcgaag tcgcacttgc ctgtaagtcg
     1801 cggatcgcga tcgatggcgc ccgctttggt tttcccgaag tgatgctcgg cctgcatccg
     1861 gggcttggcg gcaccgcgcg gttcacgcgt ctggtcaatc cgatgcaggc gatgaccttg
     1921 atgctgaccg gcaagaccat cgacgcgcgc agggccaagt cgctcgggct ggtcgatgcg
     1981 gtggcgcagg agcggcatgt ccgcaacgcc gtgaaggacg cggtgttcgg ccggttgaaa
     2041 cctgccaaac ccggtccgct gaacgcgatc ctgaatttca cgccggtgcg cggttttctc
     2101 gcctcgcgga tgcgcgcgga ggccgaaaag gccgccccgc atgagcatta tcccgcgccc
     2161 tatgcgctga tcgatctctg ggaaaaacac ggcggcgaca cgcctgcgat gctggcagcc
     2221 gaaaagacct cgttcgccaa cctgatggtg acgccgaccg cgcaaaacct gatccgggtg
     2281 ttcttcctgc gcgagcagat gaaaaaactc gccggcagtg gcaacaagat cggccatgtt
     2341 cacgtcatcg gcgccggcgc gatgggcggc gacatcgcgg cgtggtgcgc caatcaggac
     2401 atgcgggtca cgctagcgga catgaagccg gagccgattg ccggtgcggt gaagcgcgcc
     2461 gcggatttgt tcggcaagat catgcgcaag cgcacggacg tgcgcgacgc gctggaccgg
     2521 ctcatgcccg atctcgacgg cgagggcgtc cgtggggccg atttgatcat cgaggcggtg
     2581 ccggaaaaac ttgaactgaa acagaaagtc tatgccgggc ttgagccgaa aatgaagccg
     2641 ggcgcgatcc ttgcgaccaa tacctccagc attcccttgc aggacttgcg cacgacgctt
     2701 gataaaccgg cgtggctgct gggactgcat ttcttcagcc cggtatcgcg gctgcaactg
     2761 gtcgaggtcg tcagccacga cgggacagat ccgcagatgt tgaaggaggc gctggccttt
     2821 gtcgacgcca tcgaccggct gccgctgccg gtcaagagtt cgccgggctt tctcgtcaac
     2881 cgcgcgctga cgccttatat gctcgaggcg atggtgatgc tcgatgagaa aatcgacaaa
     2941 ctcaccatcg atgcggcggc ggaaaaattc ggcatgccga tggggccgat cgaactcgcc
     3001 gatcaggtcg gccttgatat ctgtctggcg gtcggcgaca tgctgcgttc gaaattcggc
     3061 gatgccctgc cgccgacgcc ggcctggctg cgcgaaaagg tcgccgccgg cgatctcggc
     3121 cgcaagaccg gcaggggttt ttatgtctgg aaggacggca aggcggaaaa aatgtccgga
     3181 tcgcccgcga tcgtgcagcc tagtccggag atgatcgacc ggctgatcct gccgatgtcg
     3241 aatgtctgcg tcgcctgtct gcgcgagggc atcgtcgaca atgccgatat ggtcgacggc
     3301 gcgatgattt ttggtaccgg ctatgcgccg tttcgcggcg gcccgctgaa ttatgcgcga
     3361 acccgcggcc ccgaaaatgt agtgtcggcg ttgcgcgcgc tagtgacaaa attcggcggc
     3421 cggttcacgc cggatgccgg ttgggagaat ttcaagtgac cgacacccag gccgttcacg
     3481 ctccgccgac ttcggaaacc gagccttgcg gcgatctctg tacccgcacg ctggcgatgc
     3541 cggccgatac caaccagaac ggcgatatct ttggcggctg gctgctcagc cagatggata
     3601 tcggcggcgg aatttttgct tcaaagatgg cgaagtcgcg caccgtcacg gtcgcgatcg
     3661 aagccatgaa tttccgcaaa cccgtctttg tcggcgatct ggtttcggtg cacgccaatc
     3721 tggtgcggat cggcaggacc tcggtcaccg tccacctcga ggcctgggtg ctgcgccgca
     3781 aggaaatgca ttcgatcctg gtgaccgacg gcaattttac cta
//
LOCUS       DASWGM010000915         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_389227,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000915 DASWGM010000000
VERSION     DASWGM010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389227"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VGA15_24835"
     CDS             <1..114
                     /locus_tag="VGA15_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9590952.1"
                     /translation="IIEALEERDTELAEKRARDHTLGLAAYVEAHGQELFS"
     gene            195..1934
                     /gene="oxc"
                     /locus_tag="VGA15_24840"
     CDS             195..1934
                     /gene="oxc"
                     /locus_tag="VGA15_24840"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEX9590953.1"
                     /translation="MSATVQSIDAKATEAERELTDGFHLMIDALKLNGLNTIYGVPGI
                     PITDFGRMAQAAGIRVISFRHEQNAGNAAAIAGFLTKKPGVCLTVSAPGFLNGLTALA
                     NATTNCFPMILISGSSEREIVDLQQGDYEEMDQLAIAKPLCKAAFRVLHAADIGIGLA
                     RAIRAAVSGRPGGVYLDLPAKLFSQVMDADAGAKSLVKVIDPAPAQLPDPAAVKRALD
                     VLKSAKRPLIILGKGAAYAQADDAIRALVEKSGIPFLPMSMAKGLLPDTHSLCAGAAR
                     STVLKDSDVVMLIGARLNWLLSHGKGKSWGEAPKKFIQIDIEPKEMDSNVEIVAPVVG
                     DIGSCVSALLEGIDGKWPAPPADWTGAVKAKRDENVAKMAPRLMNNASPMDYHGALGA
                     LRTIVKERPDAILVNEGANTLDLARGVIDMYRPRKRLDVGTWGVMGIGMGYAIAAAIE
                     TGKPVLAVEGDSAFGFSGMEVETICRYNLPVCVVIFNNDGIYRGTDVNPTGGSDVAPT
                     VFVKGARYDKMMEAFGGIGVHATTPDELKRAVNAAMDSGKPTLINAAIDPAAGSESGR
                     IGNLNPQSKLRKK"
BASE COUNT          445 a          665 c          657 g          366 t
ORIGIN      
        1 atcatcgaag cgctcgaaga gcgtgacacc gagcttgcgg aaaagcgggc acgcgatcac
       61 acgctcggcc ttgccgctta tgtcgaagcg cacggccaag agctatttag ctgaccacag
      121 ccttttattc tggacgacga cttttggaag acgacgcacg cgtccacatc aagaaacgat
      181 cagggagaag cggaatgtcc gcgacagtac agagcatcga tgcaaaagca accgaagcag
      241 agcgggagct gacggacggc tttcatctca tgatcgatgc gctcaagctc aatggcctca
      301 acacgatcta cggcgtgccc ggcattccga tcacggactt cggtcgcatg gcacaggcgg
      361 cgggcatccg cgtcatctcg ttccggcacg agcaaaacgc cggcaacgcc gcggcgatcg
      421 ccggcttcct gaccaagaaa cccggcgtct gccttacggt gtcggcgccc ggctttctca
      481 acggtctgac ggcactggcc aacgccacca ccaactgctt cccgatgatc ctgatcagtg
      541 gctcgtccga gcgcgagatc gtcgatctgc agcagggcga ttacgaagag atggaccagc
      601 tcgcgatcgc caagccgctc tgcaaggccg cgttccgcgt gctccatgcc gctgatatcg
      661 gcattggcct cgcgcgcgcg atccgcgcgg ctgtctcggg gcgtccgggc ggcgtctatc
      721 tcgatctgcc cgcaaaactt ttctcgcagg tgatggacgc cgacgccggt gcgaagtcac
      781 tggtcaaggt gatcgacccg gcgccggccc agcttccgga tcccgccgcg gtcaagcgcg
      841 cgctcgatgt gctgaaaagc gcaaagcgtc cgctgattat tctcggcaag ggcgccgcct
      901 atgcgcaagc cgatgatgcg atccgcgcat tggtcgagaa gagcggcatc cctttcctcc
      961 ccatgagcat ggccaagggg cttttgcccg acacccattc gctatgcgcc ggtgccgcgc
     1021 gctccacggt gctgaaggat tccgatgtcg tgatgctgat cggcgcgcgc ctgaactggc
     1081 tgctgtcgca cggcaagggc aaaagctggg gcgaggcgcc gaagaagttc atccagatcg
     1141 acatcgagcc caaggagatg gactctaacg tggagatcgt tgcgcctgtg gtcggagaca
     1201 tcggctcctg cgtatcggct ctgctcgaag gtatcgacgg caagtggccg gccccgccag
     1261 ccgactggac cggcgcggtc aaagccaaaa gagatgagaa cgtcgccaag atggcgccga
     1321 ggctgatgaa caatgcttcg ccgatggatt accacggtgc gctaggtgca ttgcggacca
     1381 tcgtcaagga gcggcccgat gccatcctcg tcaacgaggg cgccaacacc ctcgatctcg
     1441 cgcgtggcgt catcgacatg tatcggccgc gcaaacgcct ggacgtcggc acctggggcg
     1501 tgatgggcat cggcatgggc tatgccattg cggctgcgat cgagaccggc aagcccgtgc
     1561 tcgccgtcga gggcgacagc gcgtttggct tctccggcat ggaggtcgag acgatctgcc
     1621 gctacaacct gccggtctgt gtcgttatct tcaacaatga cggcatctat cgcgggaccg
     1681 acgtcaatcc gaccggtggt tcggatgttg ccccgaccgt gttcgtcaaa ggcgcgcgct
     1741 acgacaagat gatggaagcc ttcggcggca tcggggtcca tgcgacgacg cccgacgaat
     1801 tgaagcgcgc cgtgaacgcg gccatggact ccggcaagcc gaccctgatc aacgcggcga
     1861 tcgaccccgc ggccggcagc gagagcggca gaatcggcaa cctcaatccg caaagcaagc
     1921 ttcgcaagaa atgagacagg taacccatca acgtcattcc ccgcgcaggc ggggaatcca
     1981 gtagccaccg aactatcatg agaccaaggc tgtacccgac tgaatcgacg gtgattactg
     2041 gatgtccgct ttcgcgggca tgacgacagc agatctaagg agaaagacac atgacaaagg
     2101 cgctgaaggg cgttcgcatt ctcgatttca ccc
//
LOCUS       DASWGM010000916         7634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1206251,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000916 DASWGM010000000
VERSION     DASWGM010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206251"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VGA15_24845"
     CDS             complement(<1..597)
                     /locus_tag="VGA15_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX9590954.1"
                     /translation="MAHSILVVDDDPHIRDVVRFAFEKTGMLISTAQDGKEALRQFDR
                     NVHELVVLDIGMPEMDGLEVCRQIRKTSDTPILFLSARDEEIDRILGLEIGGDDYVTK
                     PFSPRELVARVNAILRRARTTAAPAAPKAMNQGGLTVDPDGRSVVFQDKPVSLTALEF
                     DILKTLLARPGFVFTRELILDAAYAGNIHVADRTIDSHV"
     gene            complement(603..2114)
                     /locus_tag="VGA15_24850"
     CDS             complement(603..2114)
                     /locus_tag="VGA15_24850"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEX9590955.1"
                     /translation="MSIATASDFEIAPTRPLLRPLISATACVALADWLFYGWQIGISL
                     ALFFGVFGVIAAASNGVHRTRNIQIVMSAAFVAGLLALIEDVNMLSVIVSALATAMFV
                     IVITAEETSSWQRYLFAAATTPLRGPFRLAGDVFGALRHMKTWTPGWLGSLIAWIVPL
                     SVFAVFLALFSSANPLIENRLMQVDLRAFFNLLDASRIWFWILVACTIWPLLRRRIRR
                     KPTPEPEPRAANAVESSDLDYLLGIQAVTRSLILFNALFALQTGLDLVYLWGGVTLPD
                     GMSHAEYAHRGAYPLIVTALLAAGFALIAMRPGGPAEQSRLIRPLVLVWTGQNILLVV
                     SSILRLDLYVAAYSLTYLRLAALIWMGLVAAGLLLMLIQIALKKPISWLVTANAATLA
                     LVLYGCCFLNAPQLIASYNIEHSQELGGTGPSLDLIYLAGLGPQALPALEAHIKQIPA
                     LGPIAQDRRNCRGHALSLHPANWRRWSFRAWRLERYLSNNPCMPSYLSKSDKG"
     gene            complement(2280..3188)
                     /locus_tag="VGA15_24855"
     CDS             complement(2280..3188)
                     /locus_tag="VGA15_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9590956.1"
                     /translation="MELRHLRYFVAVAEEGSLTVAAQKRLHTAQPSLSRQIHDLELEL
                     GVQLLIRGPRGIELTASGRVFLDHARVALLQVEAATEAARRAAQPARTSFAIGFLTGY
                     EMDWLPAVMGILRAELPSTEVIIHSQDSPDLAAGLMRGKIDLAFLRPEKQAPGLKFRP
                     LRKDPLIVLMPHDHALTARGSVRPQDIAGQTFIGVSATRAPTLRAVIDDYARRTGLDL
                     KPDHQAENLAMAISLVASTGGVSLLPLYAKNLLPKTVVSRPVQGAPPMVDLVIGYNEA
                     NTSPLLKFLLSRLDELKFRVPKHDGQ"
     gene            3366..4112
                     /locus_tag="VGA15_24860"
     CDS             3366..4112
                     /locus_tag="VGA15_24860"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9590957.1"
                     /translation="MNHPVVLITGALTGIGRATALAFAQEGASVVVSGRRDDVGEALV
                     AELRALGAEAEFIRADVRHESDVQNLVDKTVARFGRLDVAVNNAGTEGKPGPVTEQTA
                     DSYAATFDTNVLGVLLSMKHELRVMQAQGAGSIVNLSSTMGQRGAAGASLYTASKHAV
                     EGLTKSAALEAAAFGVRVNAVAPGPIETAMLDRFTGNADRKAGLVAGVPLKRAGRPEE
                     IADTIVFVASSRASFITGQIINVNGGKTAS"
     gene            4168..4914
                     /locus_tag="VGA15_24865"
     CDS             4168..4914
                     /locus_tag="VGA15_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9590958.1"
                     /translation="MTDLISRTALVTGASRGIGRATARALAASGARVIVHYGNAAGEA
                     EKLVAEIRASGGHADAVGADLSAPDGAQKLAAEVRKLVGDRLDILVANAGIATAATIE
                     DQTVEEFDRMFAVNVRAPFFLVQQLLPQLGEGSSVVLMSSLAARASVGLLPAYAATKG
                     AIDTLVKHFAALLGPRGIRVNAVAPGVIDTDMSSFVRSDEGRDTTLGMQALQRIGHPG
                     DVADVVTFLGSDAARWVTGDTIQVGGGSKI"
     gene            5001..5633
                     /locus_tag="VGA15_24870"
     CDS             5001..5633
                     /locus_tag="VGA15_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9590959.1"
                     /translation="MKLYQSNASPNSRRVRIYLGEKGISMPLVPVDLGAREQFSDAYA
                     AINPRCVVPTLVLEDGTAIGEVPAIQRYLEEIRPEPPLLGTTPKTKAQVAMWERRVEI
                     EGFGSVMEAVRNAVPALKGRAIAGPHDYEQIPALAERSRQRVGHFYADFNARLADVPF
                     VAGDEFSVADITAVVAVDFATKAAELPIRGEHTALKRWYDLVSARPSMGA"
     gene            complement(5646..6245)
                     /locus_tag="VGA15_24875"
     CDS             complement(5646..6245)
                     /locus_tag="VGA15_24875"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9590960.1"
                     /translation="MARKSQPKRDTFRHGNLPEALVDAALLRLEAEGVESISLRDLAR
                     DAGVNHRAVYRHFPDKLSLLAVVAATGWQRLGQRLQKSTAGKAPGEPALIAGGVGFFL
                     FARDFPNLFHLMAGARINTEGKFPDLEAAIVDALQIFAVGFAGTGMAPNIVVHRTAVF
                     VAALQGVTTQILHDRLHVAPAKTKAFVADVCKMLIKGMG"
     gene            6365..6874
                     /locus_tag="VGA15_24880"
     CDS             6365..6874
                     /locus_tag="VGA15_24880"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX9590961.1"
                     /translation="MTHATVDCPDAAPDRPDVIVFPPGIPLATLAIACALQWLAPLGW
                     IANFDQGWRIAAGAFTVFVGLLTTSAGRRALLRHGTNVNPSQPTIVLVTEGVFGRTRN
                     PLYVGVSVALCGIALIFALDWLLLLIIPSCVILHVAVVRREELYLERKFGEEYRRYKA
                     RVPRYLLGY"
     gene            6941..7372
                     /locus_tag="VGA15_24885"
     CDS             6941..7372
                     /locus_tag="VGA15_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590962.1"
                     /translation="MIKSLFRISVSLGLVGIVLGIVMGIRQDFALMPAHAHLNLLGFV
                     TLFLSALYYRVVPEAAASRLARYQAIVSVAGAVVFPMGIACVLLGGHERFEPVVVAGA
                     LTVLLGMVLFAVIVFRSSGQERAMPVGAEQRQAVFQFENEA"
     gene            7462..7634
                     /locus_tag="VGA15_24890"
                     /pseudo
     CDS             7462..7634
                     /locus_tag="VGA15_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497461.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1461 a         2359 c         2419 g         1395 t
ORIGIN      
        1 gacgtggctg tcgatggtgc gatcggcgac atggatattg ccggcatagg cggcatcgag
       61 aatgagctcg cgcgtgaaca cgaatcccgg ccgtgccagc agcgttttca aaatgtcgaa
      121 ttcgagcgcc gtcagcgaaa ctggcttgtc ttggaagaca acgctgcgcc catccggatc
      181 gaccgtgagc ccgccctggt tcatggcctt cggcgcggcc ggcgcagccg tggtccgcgc
      241 ccgccgcagg atcgcgttca cccgtgccac cagttcgcgc gggctgaacg gttttgtcac
      301 gtagtcgtcg ccgccgattt cgaggccgag aatgcgatcg atctcctcgt cgcgggccga
      361 cagaaacagg atcggcgtat cggaggtttt ccggatctgg cggcagacct ccagcccatc
      421 catctccggc atgccgatgt cgagcacgac aagctcgtgg acgttgcggt cgaactgccg
      481 caacgcctcc ttgccatcct gcgcggtgga aatcagcatg cccgtttttt cgaacgcgaa
      541 gcgcacgaca tcgcgaatat gcgggtcatc gtcgacgacc agaatggaat gagccacgtt
      601 tgtcagcctt tgtcgctttt cgaaagatac gacggcatgc agggattgtt actcaaatac
      661 cgttccagac gccatgcccg gaaactccat ctccgccaat ttgccggatg cagtgaaagg
      721 gcatgacccc gacaattccg gcggtcctgg gcgatcggcc cgagcgcggg gatttgcttg
      781 atatgcgcct ccagggccgg caaagcctga ggaccaagcc ccgcaagata gatcaaatcg
      841 agagacggcc ctgtcccgcc gagttcctgc gaatgttcga tattgtagga cgcaatgagc
      901 tgcggcgcgt tgaggaagca gcatccgtat agaacaaggg ccagcgtggc ggcgttggcg
      961 gtgaccagcc aggaaatggg ctttttcaat gcgatctgga tcagcatcag cagcagtccg
     1021 gccgcgacga gccccatcca gatcaatgcc gcgagccgca gataggtcag cgagtaggcg
     1081 gcgacataca gatcgagtcg caggatcgac gagaccacaa gcaggatgtt ttgtccggtc
     1141 cagaccagaa ccagcggacg gatcagcctg gactgctccg cgggcccgcc cgggcgcatc
     1201 gcgatcaacg caaaaccggc ggcgagcagc gccgtcacga tcaaagggta ggcgccccgg
     1261 tgggcatatt cggcgtggct catgccgtcg ggcagggtca cgccacccca gagatagacc
     1321 agatcaagcc cggtctgcag cgcgaacagc gcgttgaaaa gaataagcga gcgcgttacg
     1381 gcctgtatgc cgagaagata gtccagatcg gaagactcga cggcgttggc ggcgcggggt
     1441 tcgggttctg gagttggttt gcgtcggatg cgccgccgga gcaaaggcca gatggtgcaa
     1501 gcgaccaaga tccagaacca gattcggcta gcgtcgagaa ggttgaagaa ggcgcggagg
     1561 tcgacctgca tcaagcgatt ttcgatcaat ggatttgccg acgagaacag agccaggaag
     1621 actgcgaaga cgcttaaggg gacaatccag gcaatcagag aacccagcca ccccggagtc
     1681 caggttttca tgtgtcggag tgcgccgaac acatcgccgg ccagcctaaa aggaccgcga
     1741 agtggcgtcg tcgcggctgc gaacagatat ctctgccacg acgaggtttc ctcagcggtg
     1801 atgacgatga cgaacatcgc ggtcgccagc gcgctcacga tcacggacaa catgttgacg
     1861 tcctcgatca gggccagcag cccggcgaca aatgcagccg acatgacgat ctgaatgttg
     1921 cgcgtcctgt gcacgccatt gctggcggcg gcgatcacgc cgaacacgcc gaaaaacagg
     1981 gctagcgaga tgccgatctg ccagccgtaa aacagccagt ccgccagcgc aacgcacgcg
     2041 gtggcggata tcagcggcct caacagcggg cgagtgggag caatttcgaa atcggacgcg
     2101 gtcgcgatcg acatggtttt atgttcctga tgtgattttc cccatgaccc gacgatgccc
     2161 cgccgattgg cagcgcatat gtgcggcatg ctcagaattt gcggattttt gtgcagattt
     2221 cgcgcagacg cggggaccgt ctgaacgcag ggcgggcgac gcggcgaaga agccgcgatc
     2281 tattgcccgt cgtgctttgg cacgcgaaac ttcaattcat ccagcctgga gagcagaaat
     2341 ttcagcagcg gcgatgtgtt ggcctcgttg tagccgatga cgaggtcgac catgggaggc
     2401 gcgccctgaa ccggacggct gacgacggtt ttcggcagca ggttcttggc atagagcgga
     2461 agcaggctga cgccgccggt ggacgccacc agcgaaatcg ccatcgccag attttcggcc
     2521 tggtggtcgg gctttagatc aaggccggtg cgcctggcgt agtcgtcgat taccgcccgc
     2581 agcgtcggcg cgcgggtcgc ggatacgccg atgaaggtct ggccggcgat atcctgcggg
     2641 cgaacagagc cgcgcgcggt cagggcgtga tcgtgcggca tcaggacgat cagcggatcc
     2701 tttcgcagtg gcctgaattt cagcccgggc gcctgctttt ccgggcgtag aaaggcgagg
     2761 tcgattttac cgcgcatgag tcccgccgcg agatccggtg agtcctggct atggatgatg
     2821 acttcggtag agggtaattc ggcgcgcagg atgcccatga cagccggaag ccaatccatc
     2881 tcgtaaccgg tcaggaaacc gatggcgaat gatgtcctcg ccggttgcgc cgcccgtcgc
     2941 gccgcctcag tggccgcctc gacttgcagc agcgccaccc gcgcgtgatc gagaaacact
     3001 cttcccgatg cggtcagttc gatcccgcgc ggaccgcgga tcagcaactg aacgccaagc
     3061 tccagttcga gatcgtgaat ctgccggctc agcgaaggct gcgcggtgtg cagccgtttt
     3121 tgcgccgcca ccgtcagact gccttcttcg gccacggcga cgaagtatcg gaggtgtcgc
     3181 agctccatcc cagccattcc ttaaaagtat ggcgtcgagc ttacaaagtc tttctccggg
     3241 atgccagcga gatctagata tcctccaacc gcagcaacaa agcgccagtc gagccgcgca
     3301 gccgctccga acccgaattc gtcgcaaggc tcgacaggca tagctgacat tgaaggagaa
     3361 accaaatgaa ccatcccgtt gtactgatca ccggcgcgct gaccggcatc ggccgcgcca
     3421 ccgcgctcgc tttcgcgcag gaaggcgcca gtgtcgtggt ttccggccgc cgcgatgatg
     3481 ttggcgaagc gctggtcgcc gaattgcgcg cgcttggcgc ggaagcggaa ttcatccgcg
     3541 ccgatgtccg ccacgaaagc gacgtgcaga acctggtcga caagacggtc gcccggttcg
     3601 gccgcctcga cgtcgccgtc aacaacgccg gcaccgaggg caagcccggc ccggtgaccg
     3661 agcagaccgc cgacagctac gccgccacct tcgacaccaa cgtgctcggc gtgctgctca
     3721 gcatgaagca cgagctgcgg gtgatgcagg cgcaaggagc tggcagcatc gtcaatttgt
     3781 cgtcgaccat gggccagcgc ggcgcggccg gcgcctcgct ctacaccgcc agcaagcacg
     3841 ccgtcgaagg gctgacaaaa tcggcggcgc tggaagccgc cgcgttcggc gtccgtgtca
     3901 atgcggtggc gccggggccg atcgagaccg cgatgctcga tcgcttcacc ggcaacgccg
     3961 accggaaagc cggcctggtg gcaggcgtgc cgctgaagcg cgccggccgg cccgaagaga
     4021 tcgccgacac gatcgtgttc gtcgcctcga gcagggcgtc gttcatcacc ggccagatca
     4081 tcaacgtcaa cggcggcaag actgcttcgt aacgagaccg cttcgtaaat cttccaaaca
     4141 tttctcatcc cagaccggag aacgatcatg accgacctca tcagcagaac cgcacttgtt
     4201 accggcgcct cgcgcggcat cggccgcgcc accgcccgcg cgctggccgc cagcggcgcg
     4261 cgcgtcattg ttcattacgg caacgccgcc ggggaagcgg aaaagctggt cgccgaaatc
     4321 cgcgcgtccg gcgggcatgc cgatgccgtc ggcgccgatc tttccgcacc tgatggcgcc
     4381 caaaaactgg cggcggaagt ccgcaagctg gtcggcgacc ggctcgatat cctggtcgcc
     4441 aatgccggga tcgccaccgc tgcgaccatc gaggaccaga cggtggaaga gttcgaccgg
     4501 atgttcgccg tcaatgtccg cgcgccgttc ttcctggtgc agcaattgct gccgcagctc
     4561 ggcgagggtt ccagcgtcgt gctgatgtcg tcgctggcgg cgcgcgcgtc ggtcggattg
     4621 ttaccggcct atgccgcgac caagggcgcg atcgacacgc tggtgaaaca ctttgccgcg
     4681 ctgctggggc cgcgcggcat ccgcgtcaat gccgtggcac ccggcgtgat cgacaccgac
     4741 atgtcgagct tcgtgcgatc cgacgagggc cgcgacacca ccttggggat gcaggcgctg
     4801 cagcgcatcg gtcaccccgg cgatgtcgcc gatgtcgtca ctttcctcgg ctccgacgcc
     4861 gcgcgctggg tgaccggcga caccatccag gtcggtggcg gctccaaaat ctgagggggg
     4921 acggccgcgg cgacaaccgc tgctggccgt tccaggcgat cgccccacca gtacgtctca
     4981 ggctttatct ggagatcaaa atgaaactct accaatcgaa cgcatcgcca aattctcgcc
     5041 gggtgagaat ctacctcggc gagaaaggga tttcgatgcc gctggtgccg gtcgatctcg
     5101 gcgccaggga gcagttctcc gacgcctatg ccgcgatcaa cccgcgctgt gtcgttccga
     5161 ccctggtgct ggaggatggc accgcgatcg gtgaagtgcc ggccatccag cgctacctcg
     5221 aggagatccg tcccgagccg ccgctgctcg gaaccacgcc gaagaccaag gcacaggtcg
     5281 caatgtggga acgccgcgtc gagatcgagg gatttggctc cgtgatggaa gcagttcgca
     5341 acgccgtccc cgctctcaag gggcgcgcga tcgccggacc gcatgattac gagcagatcc
     5401 cggcgctcgc cgagcgaagc aggcagcgcg tcggccattt ctacgccgac ttcaacgccc
     5461 ggctcgccga cgtgccgttt gtggcgggcg atgagttctc ggttgccgac atcaccgctg
     5521 tggtcgccgt cgacttcgcg accaaggcgg ccgagcttcc gatcaggggc gaacatacgg
     5581 cattgaagcg ctggtatgat ttggtctcgg cgcggcccag tatgggggct tagctgttgc
     5641 tggtgttatc ccatcccctt gatcagcatt ttgcagacgt cggcgacgaa cgccttggtt
     5701 ttcgcgggcg ccacatgcag ccggtcgtgc aagatttgcg tggtgacgcc ctgcagcgcc
     5761 gccacgaaca ccgcggtgcg atggaccaca atgttgggcg ccatgccggt gccggcaaag
     5821 cccaccgcga agatttgcag ggcatcgaca atggctgcct caagatcggg aaatttgcct
     5881 tcggtgttga tccgggcgcc ggccatcaga tgaaacagat tcggaaaatc ccgcgcaaac
     5941 aggaaaaatc cgacaccgcc cgcaatcagc gcgggttcgc ccggcgcctt gccggcggtg
     6001 gatttctgca gccgctgccc gaggcgctgc cagccggttg ccgcgacgac ggccagtaac
     6061 gacaatttgt ccgggaagtg acgatagacc gcgcggtggt tgacgccggc gtcgcgggcg
     6121 agatcgcgca ggctgatcga ttccacgccc tccgcctcga gtcgcagaag tgccgcatct
     6181 accagcgctt ccggcaaatt tccatggcga aaggtatcgc gcttcggctg cgattttctg
     6241 gccattgctc agtccccgcg gatgctgcgg caatttatca ccccttgacg gccaggcgaa
     6301 gttttttcat aggatgtctc cattggagac aattgcccgt tattaccgaa tacgaggaga
     6361 aatcatgacc catgccactg ttgattgccc ggatgccgct ccggatcgcc ccgatgtcat
     6421 cgtctttccg cccgggatcc cgcttgccac gctcgccatc gcctgcgcgc tgcaatggct
     6481 ggcgccgctt ggatggatcg cgaacttcga tcaggggtgg cggatcgcag caggcgcgtt
     6541 cactgtattt gtcgggttgc tgacgacgtc agcggggaga cgcgcgctgc tgcgtcacgg
     6601 caccaacgtc aatccgtcac agcccaccat tgtgctcgtc accgagggcg tcttcggacg
     6661 aacccgcaat ccgctgtatg tcggcgtatc cgtagcactg tgcgggatcg cgctgatttt
     6721 cgcactcgac tggttgctgc tgctgattat tccgagctgc gtcattctgc atgtcgccgt
     6781 cgtcaggcgc gaagagcttt atctcgaacg gaaattcggt gaagaatacc ggcgctacaa
     6841 ggcgcgcgtg ccgcgctacc tcttgggcta ttgagcaacg cagcgttgct gcccacgctt
     6901 tgcaacaaaa tcattggcaa gaaaatcaaa ggacaagatc atgatcaagt cgcttttccg
     6961 catcagcgtt tcactgggcc tggttggcat cgtgctcggc atcgtcatgg gcatccggca
     7021 ggattttgcg ctgatgccgg cgcatgcgca cctcaatctg cttggcttcg tcaccctgtt
     7081 tctctcggcg ctgtattatc gcgtcgtgcc ggaagccgcg gcgagcaggc ttgcccggta
     7141 tcaggccatc gtcagcgtgg ccggcgccgt cgtgttcccg atggggatcg cctgcgtgct
     7201 gctgggcggg catgagcgct tcgaacccgt tgtggtagct ggcgcactca cggttttgct
     7261 cggcatggta ttgttcgcgg tgatcgtgtt ccgcagctcg gggcaggaac gcgcgatgcc
     7321 cgtcggtgcc gaacagcggc aagcggtttt tcagttcgaa aatgaggctt gaggccagcg
     7381 ctgcgatgat gcagacaccg gccgatacca gaagtgacga cagtggatga tcgtcacgaa
     7441 taaaacgccg gggccgcagc gcatctcacg ctcgggcagg cgctggcttc gccaaaagtg
     7501 atcacgctga gcctgatcta tttcggtttc gtcggcgcgc tctatggcat gtagttctgg
     7561 ttgccgcaga tcgtgaaggc cttcggcttc tccagcgcgc agaccggttt tgtcaccgcg
     7621 gtgccgctag ctag
//
LOCUS       DASWGM010000917         6582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2196942,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000917 DASWGM010000000
VERSION     DASWGM010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196942"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1306)
                     /locus_tag="VGA15_24895"
                     /pseudo
     CDS             complement(108..1306)
                     /locus_tag="VGA15_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BtaA family protein"
     gene            1470..2171
                     /locus_tag="VGA15_24900"
     CDS             1470..2171
                     /locus_tag="VGA15_24900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9590963.1"
                     /translation="MPALRADAEANTSWPVEATDRMNRMYRWQRHIYDATRRYYLLGR
                     NQLISGLRPAVGANVLEIGCGTGRNLVLAARRHPEARFFGIDVSTEMLTSAVSAISRR
                     GLTNRIRVAHGDATGFDPEFLFGIRSFDHILISFSLSMIPDWRRVLDGAVSHLKPGGR
                     LHIVDFGAQERLPGFVRAALLRWLAIFDVTPRDDLERVLAAMADASGAKLKFERPFQG
                     YAQYAVLKVPTAGIT"
     gene            2168..2491
                     /locus_tag="VGA15_24905"
     CDS             2168..2491
                     /locus_tag="VGA15_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX9590964.1"
                     /translation="MSAWLALLSAGLLEIAWALGLKCSDGLTRFWPTAATVVAIAASF
                     VLMAVALKSLPFGTAYAVWTGIGAVGSIIVGILIYSEPADPVRIVCLALIVAGMVGLK
                     LNSPV"
     gene            complement(2505..3446)
                     /locus_tag="VGA15_24910"
     CDS             complement(2505..3446)
                     /locus_tag="VGA15_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEX9590965.1"
                     /translation="MRARLKGLGIIAGIAIAVAAVFALTHILKHIDYAEVLEAVRRTN
                     ASHIVLALMLVAVSYGSLTLYDVLALRTIERTDVPYRIAALASFTSYPIAHGVGAVSL
                     VSPVIRYRIYSWNGLGVVDVANICFLTGLTFWLGNLTALALSLLAEPGAISHLEYLAP
                     PVTRLLAVALLLGIVGFLLWSWLSPRSFGNRRWSVRLPSGPVVLLQIAIAIVDLGAAA
                     LAMYVLIPSGMNIGIFRVTAVFIAAILLGFASHAPAGIGVFDATILIGLGDADKEPLI
                     AALLVFRVLYHLLPFVIALGLLGVVEAMRVLRARNLP"
     gene            3714..3935
                     /locus_tag="VGA15_24915"
     CDS             3714..3935
                     /locus_tag="VGA15_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590966.1"
                     /translation="MNTELDYDLTPDQWEALKALRAPSLKTGALNRFVLEDLIVLRLA
                     AISDDLPVITPWGRKVLVRGSTRLWDVAA"
     gene            complement(3970..4428)
                     /locus_tag="VGA15_24920"
     CDS             complement(3970..4428)
                     /locus_tag="VGA15_24920"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9590967.1"
                     /translation="MSTPKGPTLAVLPMTKDEPSAAGPQLLQPSGWPAPKGYANGMAA
                     DGRLVLTGGVIGWDSQGHLPAEFVAQVRQTLSNISAILGEGGARPEHLVRLTWYVVDM
                     EEYLANLKTLGQVYRDIFGAHYPAMALVQVVRLVEKAARVEIEATAVVPR"
     gene            complement(4452..6182)
                     /locus_tag="VGA15_24925"
     CDS             complement(4452..6182)
                     /locus_tag="VGA15_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX9590968.1"
                     /translation="MSGGIPSVGIQSLGIQSLGIQSLGPSGHVDDFTRRNLPPFHQWP
                     ELLLDRPEFRYPEYLNAAVELTDRIVEKGLGDRIALIGNGRQRTYKELADWSNRLAHA
                     LVEDYGVKPGNRVLIRSGNNPALVAAWLAATKAGAVVVNTMPMLRASELTKIVDKAEI
                     GLALTDSRIADELVACAKTSRFLKQVVNFDGTSNHDAELDRVALNKPVRFDAVKTGRD
                     DVALLGFTSGTTGEPKATMHFHRDLLIVADGYAREVLNVTPDDVFVGSPPLAFTFGLG
                     GLAIFPLRFGATATLLENAAPAEMVKIIETYKATICFTSPTAYRAMMAAMDKGADLST
                     LRLAVSAGETLPAPVFESWTRKTGKTILDGIGSTELLHIFITNRLGDAVAGTTGLPVS
                     GYEAKIVDDDMNELPPGTVGKLAVRGPTGCRYLADQRQSNYVRNGWNLTGDTFIRDQN
                     GRLSFVARSDDMIVSAGYNIAGPEIETALLTHPAVAECGVVGAPDEARGMIVKAYVVL
                     IPGVTGDAALTNDLQEHVKREIAPYKYPRAIEFVAQLPKTETGKLKRFALRQIAQAGA
                     ASASASMAAE"
BASE COUNT         1368 a         2017 c         1935 g         1262 t
ORIGIN      
        1 ggaacacatc ccggaatgac aaacgttggg tcaaatccca taggatcgtt ttcatggaat
       61 cgctcgtcta ggcgctacct acggtattac ccattcgcgc ttcaccgtca gcgccgtcca
      121 tagcagtgaa atccgccgta gattcccgat ctgtcccgcg cgaatccgat cgcgcttctt
      181 gcctcgtcgc ggcgccagat ttgccggagc ggcgcaagct cggcgttgtc cagcggagac
      241 gccgtgcccg cggtccgaaa gatcacgcgg acgttgttgc tgccggtacg gtcgatcgca
      301 ttccagagcg cctggatttc ctccggcgcc atccagtcct gcgaatccag cagcacgacg
      361 gcatcgacct cgcgtgccgg aaggctctcc aggaacagcc gaagattggc gtgcaccgga
      421 agccggcgcc gtcgcgcata cgttcgaact gtctgcgctg caggtaagcc gggaggcagc
      481 gatcgtccgg acctgcgtat ttgcgactga gcgcctgcca ggcgaaatag ttggtctcgt
      541 tggggtgcac atcgatcagg cgcagcagcc gctcatgaag cacttcgttg agcggagcgc
      601 ctcctcccag caaggcccgc tgctggggcg ggatgccaag gctgaacagc aacgccggcg
      661 tccgggttat caggcgggcc agcggcgaat gaaacaggcg atgcaaccga tccagggcct
      721 gaacgcgcgc gggcgagccg atttcttcat gcaacaatgc cgcaaggtcg atccgtgcga
      781 gtttggccag tacatgggcc atgccgatga accggccgag cacgccgtgg cgaaagaagc
      841 cgtcggtgaa ataggcatag cgcggacggc cagtcgaatt ccgcttgtcc caatatgcgc
      901 gcgcgtccgc gccgagcacc ggtcttagcc gatcgcgata aagcccggcg ttggcggccg
      961 aggcgccttc gccgaagaat tgccagaagt cagcgtaatc ggacaaagcc cgaaggccag
     1021 cgagcttgag ctcgagcagc gaaagatgag cctcattgag atcgactgcg tacacctggg
     1081 ccggccgcgc cgtcagatac gacagcgcat tgcatccgcc ggatgaaatg gtgacgatgg
     1141 ttgatcccag cggcaactgc agcgccgcaa ggtcggcttc cggatcctcc cagatctggg
     1201 tgtagaccag acggcgaaac cagaatgcga aaagccggtc ccagatcgtc gcttcggcgt
     1261 cagtcctgct gttgcgaacc gcgtcagtga taagttgcgt gttcatccat cctccgaatc
     1321 aatctgacaa agatatctcg cgctcactgc agttttgcga cgacgggcca aaccaaaaag
     1381 acgccgagag gaacccgcgc ctgtcacacc cctgtcgcgc gaacatcgga tggaagtggg
     1441 ccatcaacgg tcaacatgag tcgcatgtca tgccagcgct ccgcgccgat gccgaggcaa
     1501 atacttcatg gccggtcgaa gcgaccgatc gcatgaacag gatgtaccgg tggcaacgcc
     1561 acatctacga tgccacgcgg cgatactacc ttctcggtcg aaaccagctg atatcgggcc
     1621 tgcggccggc cgtgggcgcc aacgtgctgg aaatcggctg cggaaccggc cgaaaccttg
     1681 tgttggccgc ccggcggcat cccgaggctc gattcttcgg catcgacgtt tcgacggaaa
     1741 tgctgacgtc cgcggtctcg gccatttcac ggcgcggcct gacaaatcga atccgcgtcg
     1801 cgcatggcga tgcaaccggc ttcgacccgg agttcctgtt cggcatccgg tcgttcgatc
     1861 acatattgat ttcctttagc ctgtcgatga tcccggactg gcgtcgcgtt cttgatggcg
     1921 ccgtaagcca cctcaagccc ggtggccggc tgcacatcgt cgattttggc gctcaggagc
     1981 gattgccggg gttcgttcgc gcggcgctgt tgcgatggct tgcgatcttc gacgtcacgc
     2041 cacgcgacga cctcgagcgc gtgctggcag ctatggccga cgcctccggc gccaagctga
     2101 aattcgaacg accgttccag ggctacgcgc aatatgcggt cttgaaagtg ccgactgcgg
     2161 ggatcacgtg agcgcatggc ttgcgctgct gtcggcgggc ctgctggaga ttgcgtgggc
     2221 cttgggtctc aaatgctccg acggcctgac ccggttctgg ccgaccgccg cgaccgtggt
     2281 tgcgatcgct gcgagctttg tgctgatggc ggtcgcgttg aagtcgctgc cattcggcac
     2341 ggcctatgcc gtgtggaccg ggatcggcgc cgtcggcagc atcatcgttg gaatcctgat
     2401 ttacagcgag ccagcggatc cggttcgaat cgtttgcctc gcgttgatcg tcgcggggat
     2461 ggtcgggctc aagctcaact cgccggtatg agtgccggaa cggttcacgg cagattcctg
     2521 gcgcgcagaa ctcgcatggc ctccaccacg ccgagcaagc cgagcgcgat cacaaaagga
     2581 agcaagtgat agagaacgcg gaagaccaga agtgctgcta tcagcggttc cttgtccgca
     2641 tcgccaagcc cgatcagtat cgtcgcgtcg aacacaccga tacccgccgg cgcgtgactg
     2701 gcaaagccca gcaatatcgc agcgatgaac acggctgtga cgcggaaaat cccgatattc
     2761 atcccgcttg ggatcagcac atacattgcg agcgctgcgg cgccaagatc gactattgcg
     2821 atcgcgatct gcagcaggac aaccggcccg gacggtaacc tgaccgacca ccgccggttc
     2881 ccgaagctcc tgggggaaag ccaactccac agcaggaagc ccacgatccc caacagcagc
     2941 gccactgcca gcaatcgcgt gaccggtggc gctaggtatt caaggtggct gatggcgccc
     3001 ggttcggcaa gcaggctgag cgcaagtgcc gtcagatttc cgagccagaa cgtaagtccg
     3061 gtcaggaagc agatattcgc cacatcgacg acgccaagac cgttccacga atagattcga
     3121 taacggatca ccggagagac cagcgaaacc gctccgacgc catgcgcaat cggatagctg
     3181 gtgaagctcg cgagagcagc aatccggtaa ggaacgtcgg tgcgctcgat cgtgcgaagc
     3241 gccagcacat catagagcgt caagctgccg tatgaaacgg ccacgagcat cagagccagc
     3301 acgatgtggc tggcgtttgt gcgacgaacc gcttccaata cctcggcgta atcgatgtgc
     3361 ttgaggatat gcgtcagcgc gaatacggcc gccacagcga tggcaatgcc ggcgatgatg
     3421 ccgaggccct tcaatcgggc gcgcaagcca tgtctcttgg gtagcacaac ggctttcaga
     3481 gcatccatcc attcgggcaa cgagtccgca atcgcgccgc ctgattcggg gagttcgatc
     3541 acgatccatc gacacgttga tgtgacagcc gcgttacccg cgaacttcca gccgaaacga
     3601 ctgtcggcga cagaatgtca cgcatcgaaa cgaattcgtg accagtttcg gtcgcacgga
     3661 cttcacgctc acccggtaag acgtgcgcga aaccaggagg atattgaggc gcaatgaaca
     3721 cggaattgga ctacgacctg acgccggatc aatgggaagc gctgaaagcg cttcgcgcac
     3781 cttcattgaa gaccggcgcg ttaaacagat tcgttcttga agatctcatc gtacttcgcc
     3841 ttgcggcgat cagtgacgat ttgccggtca ttacaccctg gggacgcaag gtcctggtga
     3901 gaggctcgac ccgcttatgg gacgttgcgg cctgagcctc cgcgccccgc gatcgcacgg
     3961 ccagaatcat caacgcggca ccaccgccgt cgcttcgatt tcgacccgcg cggccttttc
     4021 aacgagacgc acgacctgaa ccagcgccat ggcgggataa tgtgcgccga aaatgtcgcg
     4081 atagacttgc ccgagcgtct tcaggttcgc cagatattcc tccatatcga cgacgtacca
     4141 ggtgagacga acgagatgtt cgggtctggc accgccctcg ccgagaatgg ccgaaatgtt
     4201 gctcagggtt tgacggacct gcgccacaaa ctcggctggc agatgaccct gactatccca
     4261 gccgatcacg ccgccagtca gcacgagacg gccgtcggcc gccatgccgt tggcatatcc
     4321 cttcggcgcc ggccaaccac tcggctgcaa tagctgcgga cccgcggccg aaggctcgtc
     4381 cttcgtcatc ggcagcacgg caagcgtggg cccttttggc gtgctcacag acactcctcc
     4441 ctcacacttg tttattccgc ggccatgctt gccgacgccg acgcagcacc agcctgagcg
     4501 atctgccgca acgcaaatcg tttcaacttg ccggtttcgg tcttgggcag ttgcgcgacg
     4561 aactcgatcg cacgtggata tttgtaaggc gcgatctcgc gcttgacatg ttcctgcaag
     4621 tcattcgtca gcgcagcatc ccctgtgacg ccagggatga gaaccacata ggccttgacg
     4681 atcatgccgc gcgcttcgtc gggagcaccg acgacaccgc attcggcgac ggccggatgg
     4741 gtcagcaacg cggtctcgat ctcgggaccc gctatgttgt aaccggcgga aacgatcatg
     4801 tcgtccgatc gggccacgaa agacaagcgg ccgttctggt cgcggatgaa cgtatcgccc
     4861 gtcagattcc aaccattgcg tacataattc gactgccgct ggtccgcgag atagcggcat
     4921 ccggtcgggc cgcgaaccgc aagcttgccg acggttcccg gcggcaattc gttcatgtca
     4981 tcgtcgacga tctttgcctc gtaacccgag accggaagcc ccgtcgttcc ggcgacggcg
     5041 tcgcctagtc ggttggtaat gaagatatgc agcagttccg tgctgccgat cccgtcgaga
     5101 atggtcttgc ccgtcttgcg ggtccagctt tcgaaaaccg gcgccggcaa ggtttccccg
     5161 gccgaaaccg caagacgcag cgtcgaaaga tcggcgccct tgtccatcgc ggccatcatc
     5221 gcccgatacg cggtcggcga ggtaaagcaa atcgtggcct tgtaggtttc aatgatcttg
     5281 accatctcgg cgggtgccgc gttttccagc agcgtcgcgg tagcgccgaa ccgcaacgga
     5341 aagatcgcga ggccgcctaa cccaaacgta aaggcaagcg gcggcgaccc tacgaagacg
     5401 tcatccggag tgacgttgag tacttccctg gcatagccat ccgcgacgat caggagatcg
     5461 cgatggaaat gcatcgtcgc ttttggttcg cccgtggtgc cggaggtaaa tcccagcaag
     5521 gcgacatcat cccggccggt cttgaccgca tcgaaccgaa ccggcttgtt cagcgccacc
     5581 cgatcgagct cggcatcatg gtttgacgtt ccgtcaaaat tcacgacctg cttgagaaat
     5641 cggctggtct ttgcgcatgc gaccagttcg tcggcgatgc gactgtcagt cagcgcgagg
     5701 ccgatctctg ccttgtcaac gatcttggtc aattcagagg cacgaagcat cggcatggta
     5761 ttgacgacga cggcgcctgc tttcgttgcc gcgagccatg ccgcgaccag ggccggattg
     5821 ttgcccgagc gtatcaaaac ccggttgccg ggctttacgc cgtaatcctc taccaatgcg
     5881 tgcgcaaggc ggttcgacca gtccgccagc tctttgtagg tacgctgacg accgttgcca
     5941 atgagcgcga tacgatcgcc gagccccttc tcgacgatcc ggtcagtcag ttcgaccgcg
     6001 gcgttgagat attcgggata cctgaactcc ggccggtcga gcagcaattc cggccattgg
     6061 tggaacggcg gaagatttcg ccgcgtaaaa tcatcaacat gacccgatgg gccaaggctt
     6121 tgaattccaa ggctttgaat tccaaggctt tgaattccaa cgctgggaat tccacccgac
     6181 atttccatcg tccctcgtct ttgggatgaa acgcttacaa ccaatatcca aggcgtccga
     6241 cagcgcttta tattcccaaa cattttatac ttaaagtaat ttggcgcaat agcccaaatc
     6301 cgggcaatcc aacacttttt tgaaacctag cgcgtggcct atgtcctttg gcaccaaacg
     6361 gacatgctgg ctgcattgct caatgtccgc tcggggcaaa gcggaaacca tatgctcgca
     6421 ttgggctttt cagcttttta cccaggctgt gtgaaaagtc gcgtgagcca aggaagcgca
     6481 gaattgtttt ctcaattgcc gtctcccgac cgaggttgcc agcgcaattg gtttccgtat
     6541 cgatgaaact gagacagatg ttctacacgc aaattgaacg ac
//
LOCUS       DASWGM010000918         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2723275,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000918 DASWGM010000000
VERSION     DASWGM010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723275"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..379)
                     /locus_tag="VGA15_24930"
     CDS             complement(164..379)
                     /locus_tag="VGA15_24930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590969.1"
                     /translation="MYGAVALRVFAQTLKEESRLSDTGLEISLGLTVSRERQTRHWRP
                     MIVRGTLLTLTLLTSCLSPHSPDMLGI"
     gene            complement(636..1415)
                     /locus_tag="VGA15_24935"
     CDS             complement(636..1415)
                     /locus_tag="VGA15_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590970.1"
                     /translation="MPETCFPTRTRWQGLIASPGLTLFLAGVTLSGCSGGDFGRTRQD
                     FLNDDMHRWIGGEATGSVGLRASQFQLTDNERLLRDLAYPLIEPPHSRPAWKSVFGDY
                     AALPSPWRQKVVFDRTAYGRTLIDEPHRSHSSRYAQLMEDVRDDITRFEPFFASAVRV
                     IDLDRKRNASLRQISELSPRERADAIARMEENTLIVQWVQQCLEQRISSYRWALERLV
                     IQAPDNIAADADRLIGELAAQTANPPVAAQPVIGHAVSVRG"
     gene            complement(1432..1632)
                     /locus_tag="VGA15_24940"
     CDS             complement(1432..1632)
                     /locus_tag="VGA15_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590971.1"
                     /translation="MGTIIAFPAVAASRRLGSTMDGAPREGMGIILILPVVRIERDTD
                     ETSGGRGPEEGTAPGRRRRRRT"
     gene            1823..2062
                     /locus_tag="VGA15_24945"
     CDS             1823..2062
                     /locus_tag="VGA15_24945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590972.1"
                     /translation="MECDVKPFSKPWKLVERDEPFEVRDSASRTLAFVYFSDDPDRWN
                     FMRRLSTEDARRMAQQILRLPELVRIARGIDPGET"
BASE COUNT          408 a          669 c          652 g          410 t
ORIGIN      
        1 cgcgtaggcg acgtgcgaat cacgccctag gaatctaggt cgacggcgcg ccacccgggc
       61 ctcgcccgcg atgcgctgct ccacgttcag gtaagccacc atcagcgtgg tcccgcccgc
      121 cggatgcgga gccaaagacc caaggcaacg tccccgcgac cggtcagatg ccaagcatat
      181 ccggtgaatg cgggctgaga caagaggtaa gcaacgtaag tgtaagcaat gtacctctga
      241 cgatcatcgg ccgccaatgc ctggtttggc gttcgcggct gaccgtgagg ccaagcgaga
      301 tctcaaggcc ggtgtcggac agccggctct cttctttcaa cgtttgcgca aagacgcgca
      361 gcgccactgc gccatacata taaggtattt tcagtcaacg ctcttcatcg gcggaatgtt
      421 ctgcgcaatc tataaagcct cgttatcact cggcgccggt acagagagtg ccctcaaagc
      481 cctggatctc aatatccagg tcccttgatc caggttcctt gatccatgtc ccttgttggc
      541 actttttggc gcgctgttcg gcttgagtcc gtaatgcgca ccaatgccga cgtccgccaa
      601 gcggattatg agttcgcgcg ctaatagatc atgcgctagc cgcgaacgct caccgcatga
      661 ccgatcaccg gttgcgccgc aaccggcggg ttcgcggtct gcgcagcgag ttcgccgatc
      721 aaccgatcgg cgtcggctgc gatattatcc ggcgcctgga tcaccagccg ctccagcgcc
      781 cagcggtagg acgagatccg ctgctccagg cattgctgca cccactgcac gatcagggtg
      841 ttctcttcca tacgggcgat cgcgtcggcg cgttcgcgcg gcgagagttc cgagatttgc
      901 ctgaggctgg cgttacgttt cctgtcaaga tcgatcaccc gcacggcact ggcgaagaac
      961 ggctcgaacc gggtgatgtc gtcgcgcaca tcttccatca gctgcgcata gcgcgacgaa
     1021 tgcgagcggt gcggctcgtc gatcagggtg cggccatagg cggtgcggtc gaacaccact
     1081 ttctgccgcc agggtgatgg aagggctgcg taatcgccga acacgctctt ccaggccggc
     1141 cgcgaatgcg gcggctcgat cagggggtaa gcgagatcgc gaagcagccg ttcgttgtcg
     1201 gtgagctgga attgcgacgc ccgcaggccg acgctccccg tcgcctcgcc gccaatccag
     1261 cggtgcatgt cgtcgttgag aaaatcctgc cgggttcggc cgaaatcgcc gccgctgcag
     1321 cccgaaaggg tcacgcccgc caaaaatagc gtcaggcccg gggaagcgat cagaccctgc
     1381 catcttgtcc gggtcggaaa gcacgtctcc ggcatatcga aatgtccgat gtcaggtgcg
     1441 ccggcggcga cggcggccgg gggccgtacc ttcttccggt ccgcggccac cgctggtttc
     1501 atcggtgtcg cgttcgatcc ggacgacggg gaggatcagg atgatcccca tgccttcgcg
     1561 tggagcccca tccatggttg agcccagccg ccgcgaagcc gcaaccgctg gaaatgcaat
     1621 gatggtgccc atattgaact ctctctgatt gccgcgctga cacgattttg ccggcgacag
     1681 gccatcatta agaacaggac gtggttaacg gcatcttaac gctagggctt tgatttcatt
     1741 gatggcggtg tcgccctgcg cgggcaaatg cgccggaacg ttcgggattt gtgcactgac
     1801 gtcaggatgt taaactcccg gcatggaatg cgacgtcaag ccattctcaa agccgtggaa
     1861 gctggtcgag cgcgacgagc ccttcgaggt tcgggactca gccagccgca cgctcgcctt
     1921 tgtctatttc tccgacgatc ccgatcgctg gaacttcatg cgccggctgt caactgaaga
     1981 cgccaggcgc atggcgcagc agatcctgcg gctgccggag ctggtccgga tcgcccgcgg
     2041 catcgatcca ggcgagacgt gactgtcctg gtcgcgccga ttcggcgagc gacagactgg
     2101 aaaacgatta catcctcaaa agcgacgacc gctcgatcg
//
LOCUS       DASWGM010000919         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1334663,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000919 DASWGM010000000
VERSION     DASWGM010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334663"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VGA15_24950"
     CDS             complement(<1..1034)
                     /locus_tag="VGA15_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9590973.1"
                     /translation="MPTERFQFTGEGSHQLAASLDLPEREPLAYALFAHCFTCGKDVL
                     AAKRIAVALAAKGIAVLRFDFTGLGSSEGDFANSTFSSNVADLVRAADHLRETRKAPT
                     ILIGHSLGGAAILAAAVQIPDARAVVTIAAPSDPAHVTGLFRDRIDDIRKHGKVEVSL
                     AGRPFHISSEFLDDIAHHGLMAHVATLHKALLIMHAPTDDTVGIDNATDIFLAAKHPK
                     SFVSLADADHLLTGKRDASYVADVIAAWAERYLDPVISEPAVDRGAAPRSVVVRETRN
                     SKFQQTVSIGPHHLLADEPVAAGGEDTGPGPYDFVLAGLGACTSMTMRLYAERKSLPL
                     ERVTVTLNHS"
     gene            complement(1263..1928)
                     /locus_tag="VGA15_24955"
     CDS             complement(1263..1928)
                     /locus_tag="VGA15_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX9590974.1"
                     /translation="MADNSDEPPTSKLTRSKQRWAREGRFLIGKTSRPEEQRLPPGQH
                     LTKDWPTLDLGLTPNISRERWRLDVYGAVEHPIFWDFAQFTAQPQTKFISDIHCVTTW
                     SRYDNQWEGLATRDLLMACQPRDVARHVVLHSHDGYTTNLALEDFAAEDALLAHAWSG
                     APLEQEHGGPVRLVVPHLYFWKSAKWLQSIEFLAEDKPGYWEVRGYHNRGDPWQEQRY
                     SGD"
     gene            complement(1942..3264)
                     /locus_tag="VGA15_24960"
     CDS             complement(1942..3264)
                     /locus_tag="VGA15_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9590975.1"
                     /translation="MTDQALRPVPDHLDLADVERRIKAIFIGSVGNLVEWYDFYAYTA
                     FALYFAPAFFPNSDPVVQQLNAAVLFAATFLMRPLGGWLFGFIADRYGRRLSLTLSVV
                     CMCFGSLIIAVTPTYASIGLAAPAILAAARIIEGLSLGGEYGASATYLSEVADANHRG
                     FYSSFQYVTLIGGQLTAIIVLLLLQKVFLTPEELKAWGWRIPFVIGAGLAVFAAVMRR
                     NLHETEAFVEAKKLAKPTGSIRGLLKYPRELLLVVGLTAGGTAAFYTFTTYMQTFVKL
                     SVGLTEDQTTFVIFGSLIFATLLQPLYGALSDRIGRKPLLIFFGVAGTLATIPILTTL
                     KETKSPFTAFLLICAAWIFVAGYTSINAIVKAELFPTNVRALGVGLPYAITVSVFGGT
                     APAVALYFKSLGHEDWFYYYLSGMICLSLIIYATMRDTKHESAMHRHE"
     gene            complement(3307..3920)
                     /locus_tag="VGA15_24965"
                     /pseudo
     CDS             complement(3307..3920)
                     /locus_tag="VGA15_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315601.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
BASE COUNT          766 a         1181 c         1268 g          705 t
ORIGIN      
        1 ttgctgtggt tcagcgtcac cgtcacgcgc tccagcggca gcgatttgcg ctcggcatag
       61 aggcgcatgg tcatcgaggt gcaggcgcca aggccggcca gcacgaaatc atacggtccg
      121 ggtccggtgt cctcgccgcc ggcggcaacc ggctcatcgg ccagcaggtg atgcggcccg
      181 atcgaaacgg tctgctggaa tttgctgttg cgggtttcgc ggaccacaac gctgcgcggc
      241 gccgcaccgc gatcgacggc tggctccgat atgacgggat cgaggtagcg ctcggcccag
      301 gcggcgatga catcggcgac ataggaagca tcccgcttgc cggtcaagag atgatcggcg
      361 tccgccagcg acacaaaact cttgggatgc tttgcggcga ggaagatatc cgtagcgttg
      421 tcgatgccga cggtgtcgtc ggtcggcgcg tgcatgatca gcaacgcctt gtgcagcgtt
      481 gcgacatgcg ccatcaggcc gtgatgggcg atgtcgtcga ggaattcgct cgagatatgg
      541 aatggccggc cggcgagcga gacttcgacc ttgccgtgct tgcggatgtc gtcgatgcga
      601 tccctgaaca ggccggtgac gtgggcggga tcggagggcg cggcgatggt gacgacggcc
      661 ctggcatccg gaatttgcac cgcggcggcc agaatggcgg cgccgccgag actatgcccg
      721 atcaggatcg ttggagcctt gcgggtttca cgcagatggt cggcggcccg cacgagatcg
      781 gcgacgttcg acgagaacgt ggaattagca aagtcgcctt cgctggagcc gaggcccgtg
      841 aaatcgaacc gcagcaccgc gatgcctttt gccgccagcg ccaccgcgat gcgcttggcc
      901 gccagcacat ccttgccgca ggtaaaacaa tgcgcgaaca gcgcataggc cagcggctcc
      961 cgctcgggca ggtcaagcga ggccgcaagc tggtggctgc cttcacccgt gaattggaaa
     1021 cgttcagtcg gcatcgttgg cctccatggt gacggtcttc acctctccca tagggagagc
     1081 ataggcggcc tgcggccgcc gtccttagaa tagaacgcca atgctgcgca ttggctatcg
     1141 cgaagcgccg ggtgagaggt tacggcctat cgatagtccg taacccctca cccgaatttc
     1201 tcgctacgct cgaaattcga cctctcccaa agggagaggt gacgtaaact ccggcaccca
     1261 agctagtcgc cggaatagcg ctgttcctgc cacggatcgc cgcggttgtg ataaccgcga
     1321 acttcccagt agccgggctt atcctcggcg aggaattcga tgctctgcag ccattttgcg
     1381 cttttccaga aatacagatg cggcaccacc agccgcaccg ggccgccatg ttcctgctcc
     1441 agcggcgcgc ccgaccaggc gtgcgcgagc agggcgtctt cggcggcaaa atcttccagt
     1501 gccagattgg tggtgtagcc gtcatgggaa tgcagcacca cgtggcgcgc tacgtcgcgc
     1561 ggctggcagg ccatcagcag atcgcgggtc gcgagcccct cccactggtt gtcgtagcgc
     1621 gaccatgtcg tcacgcagtg gatgtctgaa atgaatttcg tctgcggctg cgccgtgaac
     1681 tgcgcaaagt cccaaaaaat cggatgctcg accgcgccat agacgtcgag gcgccagcgc
     1741 tcgcgggaaa tgttcggcgt caggccgaga tcgagggtgg gccagtcctt ggtcagatgc
     1801 tgtcccggcg gcaggcgctg ttcttcgggg cgcgaggttt ttccgataag gaagcggcct
     1861 tcgcgcgccc agcgctgctt gctgcgcgtc agttttgaag tcggcggttc gtcgctgttg
     1921 tcggccatat ctcaagcatc tttattcgtg acggtgcatc gccgattcgt gcttggtgtc
     1981 gcgcatcgtc gcgtagatga tcagcgacag acatatcatg ccgctgagat aataatagaa
     2041 ccagtcttcg tgtccgagcg acttgaaata gagcgcgacc gcgggcgcgg tgccgccgaa
     2101 cacggatacc gtaatcgcgt agggcaggcc gacaccgagc gcgcgcacat tggtcgggaa
     2161 caattcggcc ttcacgatgg cgttgatcga ggtgtaaccg gcgacgaaaa tccaggccgc
     2221 gcaaatcagc aggaaggcgg tgaagggtga cttggtctcc ttcagcgtgg tcaggatcgg
     2281 gatcgtcgcc agcgtgccgg cgacgccgaa aaagatcagc agcggcttgc ggccgatgcg
     2341 gtcgctcaat gcgccgtaga gcggctgcag caaggtcgca aagatcagcg aaccgaagat
     2401 gacaaaggtg gtctggtctt cggtgaggcc gaccgacaat ttcacgaaag tctgcatata
     2461 ggtcgtgaag gtatagaacg ccgcggtgcc gccggcggtg aggccgacca ccagcaatag
     2521 ctcgcgcgga tatttcaaga ggccgcggat cgagccggtc ggttttgcaa gctttttggc
     2581 ttcgacgaac gcctcggtct catgcagatt gcgtcgcatc accgcggcga aaacggcaag
     2641 cccggcaccg atcacaaacg gtatccgcca gccccacgcc ttcaattcct ccggcgtcaa
     2701 gaacaccttt tgcagcagca gcaagacgat gatcgcggtg agctggccgc cgatcagcgt
     2761 gacgtattga aagctcgaat agaagccgcg atggttggca tcggcgactt cgctgagata
     2821 ggtcgcgctg gcgccgtatt cgccgccgag gctgagaccc tcgataattc gcgcggccgc
     2881 gagaatcgcc ggcgcggcga ggccgatcga ggcataggtc ggcgtcaccg cgatgatcag
     2941 cgagccgaag cacatgcaga ccaccgacag cgtcagcgac agccgccggc cgtagcggtc
     3001 ggcgatgaaa ccgaacagcc agccgccgag cggccgcatc aggaaagtcg cggcgaacag
     3061 cacggccgcg ttgagctgct gcaccaccgg gtcgctgttc ggaaaaaacg ccggcgcgaa
     3121 gtaaagcgcg aacgccgtat aggcgtagaa atcataccat tcgacgagat tgccgaccga
     3181 gccgatgaag atcgccttga tccggcgttc cacgtcggcg agatcgagat gatcgggcac
     3241 cggcctcagg gcttgatctg tcacgtccgg cctccagcaa caatgaaggc agcttgttag
     3301 cagacttcag cccgcctttc caaacagcat cggaagctgg cgcggtccgc gcacggtgcc
     3361 ttccgaccag gtgacctggc ccgccggatc cagcctgaaa tcgggaatcc gcttcagcca
     3421 ttcctcgatg gcgaccgtca tttccatgcg cgcgagattg gagccgacgc agcggtgaat
     3481 gccgaggcca aaggcggcgt ggcgattctc cttgcggtcg atcacgacct tgtcggcgtc
     3541 ggggaacatg gctggatcgc ggttggcggc gggaaacgac agcagcacca tgttgccggg
     3601 ttttacgggg cagccgctga ccacggtctc cttcatcacc tcgcgggcca tcgtgaccgg
     3661 cgaataggcc cgcagcaatt cctcgaccgc ggtcggcatc agttccggtt ccgcgatcag
     3721 acgcgcgcgg tccgaaggcg ttttcgccag atgccacagc gaggaaccga tcgcgctcta
     3781 ggtggtgtcg atgcccgcga tcagcaatag ccgcagcgag cccagcacat gcgagtcgga
     3841 caagggctga ccgctcttgt ctttggcgtt catcagcgtc gaaatcagat cgtcagtcgg
     3901 ctgcttcttg cgttgttcga
//
LOCUS       DASWGM010000920         4830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2197595,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000920 DASWGM010000000
VERSION     DASWGM010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197595"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(702..1652)
                     /locus_tag="VGA15_24970"
     CDS             complement(702..1652)
                     /locus_tag="VGA15_24970"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9590976.1"
                     /translation="MHDHIDSRAAEGVQKRRPVEERIGFIGLGRMGAAMATNLVHAGC
                     DVRAYVRRPERIKQLAALGLDTTAEVADLFDCEIVVSMLPDDKAVRDVMFGLEPRPGN
                     GLASGLMPGAIHLSMSTISTAASSAIDSGHAKLDQGYVAAPVFGNPDAAAARQLYIVA
                     AGSGYYIERCQPIFDILGQRTFVVGPEPATANLIKLAGNALTAATLEMLGEVVALMRK
                     RGEDPEQFLQILTETMYGSRVHKIYGGKIVAQRYEPGFVLPLALKDVRLALAEAEAAG
                     VPMPSVRIARDRLMAGIARGYAALDWSALGRIAADEAGLT"
     gene            2282..3325
                     /locus_tag="VGA15_24975"
     CDS             2282..3325
                     /locus_tag="VGA15_24975"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX9590977.1"
                     /translation="MLANSLRTNLASNGPFDRNTSKPGQAAMLLSLDGHSRARERHDM
                     RTDPRAHRLPNGPRVDAAGHAVEIYPADIVKRRTMASNGMAGEIVQATKRGKIESRFR
                     APVHLLAVYDRGMRRDGETFVEGLPRSALRDLRRKLTFVPAGHEYYDCQDPHILTRVA
                     YFYFDPTELAIDPELGFAEMAPRLFFENAALWSTALKLMSLVEHSLSANRLYFEALGA
                     VLAHELVRLNTGAPSSVPPVRGGLAAWQQRIAVAYIEEHLGDQIPLAQLAQLVRLSPH
                     YFCRAFKQSFGMPPHRYHTSRRIERAKILLADPALSVTEIGLTAGFSETGSFSAAFRK
                     ATGLTPTGFRSLL"
     gene            3397..3657
                     /locus_tag="VGA15_24980"
     CDS             3397..3657
                     /locus_tag="VGA15_24980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590978.1"
                     /translation="MLTANELADDELLGMLDRRFWLARAGSVALGLPCTLRKALCRAY
                     AASSESSRAASIVKLANDEIRIGSGQIGRLRQRMGLAGTGSS"
     gene            4122..4622
                     /locus_tag="VGA15_24985"
     CDS             4122..4622
                     /locus_tag="VGA15_24985"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEX9590979.1"
                     /translation="MDIVDAARRREGRSRVGEINRAVNLAIRPMSDVAQYGVPDLWTA
                     PIQTLTAGAGDCEDYAIAKYVALEEAGVAEEDLRLVVVRDTRRREDHAVVAVRLDGRW
                     LVLDNRWLALVEDVELETYLPMFGMNREGVRRFVAAQQRVFSRGATEPAPAPLRSQKQ
                     PLEHAS"
     gene            4641..>4830
                     /locus_tag="VGA15_24990"
     CDS             4641..>4830
                     /locus_tag="VGA15_24990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590980.1"
                     /translation="MEMATPPDNIINSMHETFLLVIIAFIAFALIAAANGAYTNKARH
                     PRDQASDIRSGHETMMATP"
BASE COUNT          903 a         1516 c         1534 g          877 t
ORIGIN      
        1 cgaattttca ggtattttca gggtttttaa gaaggctgct ccaaatggca tgccgcctcg
       61 cggaggcgat cgtagatcgg tgccccgacg cggtgcgtca aggtcttgct cgcgtcacgt
      121 atgcagtttt cgaggcagag cggtcattga gtaagtacag tcgaagccgg cctcctcgcc
      181 gtggagcaca gtggaaagcg ggggctgccg ccgcaaccga tcgccccata gggccagcgc
      241 gtcacctgaa atcgccgcca gttccttgcc ggcggaaaaa atgtcgtgcc gttcggagat
      301 tatgacgcct cacccggctt cgttactcga caaaaaaaag ggccggcggc aggcgccacc
      361 ggcccaagtg tctacgtcca agcgaaaccc gcggggagga accaggcttc gcgacgaccc
      421 acgcagcgat caggaccagg aggccggacc cgagggatcg agcgaacgtg ggcctgacgt
      481 agacatcgct cgtatgctcg gttcgtctta aggcttcgac atcgtccgaa tgtaagcggc
      541 cttggcgcaa gctgctgcgg cgaaattgct acgattgatg cgttcttgca ccgatctgga
      601 ggctgcgctg cacttggtcc aaccttgttg atcgcggagc ggctgattac acccggcgcg
      661 ccgaagggga ggctcacggc ttccgccaca ccggtgtctt cctaggtcag tccggcttcg
      721 tcggcagcaa tgcggccgag cgccgaccaa tcgagtgccg catagccacg tgcaattccg
      781 gccatcagcc gatcgcgtgc aatgcggacc gacggcatgg ggacgcccgc ggcttccgcc
      841 tccgcgagcg ccaaacgcac gtccttgagc gccagcggaa ggacgaagcc cggctcgtag
      901 cgctgcgcga cgatcttgcc gccgtagatc ttgtgcaccc ggctgccgta catggtttcg
      961 gtcaggatct ggagaaactg ctcggggtcc tccccgcgct tgcgcatcag ggcaaccact
     1021 tcgccgagca tttccagtgt ggccgcggtc aacgcattgc cggcgagctt gatcagattc
     1081 gcggtcgcgg gttcggggcc gaccacgaag gtgcgctggc ccaaaatgtc gaagatcggc
     1141 tggcagcgct cgatataata accgctgccg gcggcgacga tgtagagctg acgggccgcg
     1201 gcggcatcgg gattgccgaa caccggcgcg gcaacgtagc cctggtcgag cttggcgtgc
     1261 cccgagtcga tcgccgacga agcggccgtg ctaatggtgc tcatcgagag atggatggcg
     1321 cccggcatca gcccggaagc aagcccattt ccgggccgcg gctccaggcc gaacatgacg
     1381 tcccggacag ccttgtcgtc cggcagcatg ctgaccacga tttcgcaatc gaacaggtcc
     1441 gcgacttcgg ccgtcgtgtc gagcccgagc gcagcaagct gcttgatgcg ttcgggacgc
     1501 cggacatagg ccctcacatc gcagccggcg tgcacgagat tcgtggccat ggcggcgccc
     1561 atgcgtccaa gcccgatgaa gccgatgcgc tcctccacgg gtctgcgctt ctggacgccc
     1621 tccgcagcgc gagaatcgat gtggtcgtgc atggaagcct caaagcatcg tgacgcctga
     1681 ttgcggtagg aacttagtcg tttccgcggc ggcggctgtg cctcgattgc cgcgtttggc
     1741 ccgctcttgc cgaaaatcga gcgtggacgc gcgatcttgc gggcctctgc cgaaccaaac
     1801 gggcgaatgc gcgaaaatcc gccgctttac gtagatttca gatgatttcg gaacgtttca
     1861 ggccgtcgcc aaggaccttt caggcgccga gcgcgatatt tcgtgcacgg acggaagatt
     1921 cgagcgccag aggtgcaacg ccatgtcgat gaacccgctg cccacgaacc ctacgccata
     1981 cgcgccgttg aactcgaatg cgctgcggcc gctgccgacg gccagggtgc cgtcggtcga
     2041 gcggcgatct cgatccggcg gaagacacga cgaccgggcc gattgggata acgctcgccg
     2101 atccgtcgaa gtcccgctgc caaaccaagg tatgaggctc tcaaaccaat acgctattcg
     2161 gatcaacctc cgtacgatgg gcggtttgcc tcgatcgaac tatgctctcc tgagttggtg
     2221 agagccggaa tggggccggg gaagtgcgag ctaaccgacg cgaacgtcaa aggtggaatc
     2281 catgttggcc aactccctgc gtacgaatct ggcatcgaat ggtcctttcg accggaatac
     2341 ctcaaagccg ggccaagccg ccatgttgct gtcgctggac ggacactccc gtgcccgcga
     2401 aaggcacgac atgcgcaccg atccccgggc tcatcggctg cccaatgggc ccagggtcga
     2461 cgctgccggt catgccgtgg agatttatcc agccgacatc gtgaaacgtc gcaccatggc
     2521 ctcgaacgga atggcggggg agatcgtcca ggccacgaag cgtggcaaaa tcgaatctcg
     2581 cttccgcgcg cctgtccacc tgctcgccgt atacgacagg ggcatgcgcc gggacgggga
     2641 gacgttcgtc gaggggctgc cccgctcggc cctgcgagat ctgagacgaa agctaacctt
     2701 tgtgccggca ggtcacgaat attatgactg ccaggacccg cacattctca cgcgcgtggc
     2761 ttacttctat ttcgatccaa ccgagctggc aatcgatccg gagctgggtt tcgcggagat
     2821 ggctccgcgg ctgtttttcg agaatgcggc gttatggagt acggcgctca agctgatgtc
     2881 cctggtcgaa cattcgcttt cggcgaaccg gctgtatttc gaagctctcg gcgccgtcct
     2941 ggcgcacgag ctggtgcggc tcaacaccgg agcgcccagc agcgtaccgc ccgtgcgcgg
     3001 cggtctcgcc gcctggcagc aacggatcgc ggtcgcgtac atcgaggagc accttggcga
     3061 ccagatcccg ctcgcccagc tcgcgcagct ggttcggctc agcccgcact atttctgcag
     3121 ggccttcaag cagtcctttg gcatgccgcc gcaccgctat cacaccagca gacgcatcga
     3181 gcgcgcgaag attctgttgg ccgatcctgc gctctcggtg acggagatcg gattgaccgc
     3241 gggcttcagc gagaccggtt cgttcagcgc ggcatttcgc aaagcgaccg gacttacccc
     3301 gaccggattt cgcagcctcc tctgagctcg gcaagactcc gcactacccg gtgagaggga
     3361 atgggagaag gggaattcgc tgagatggag acgcccatgt tgaccgcgaa cgaacttgcc
     3421 gacgacgaat tgctcggaat gttggatcgt cgcttttggc tggcgcgcgc cggctcggtc
     3481 gcgctgggct taccctgcac gctgcgcaag gctctatgtc gggcgtatgc ggcttcaagc
     3541 gaaagcagcc gcgccgcgtc gatcgtcaag ctggcgaatg acgagatccg cattggctcc
     3601 ggccagatcg gcaggctccg acaacggatg ggcctcgcag gcaccggatc cagctagcga
     3661 gactctcatc gtcagcttcg ccccgcccgt gtcgtgagag ctgcgagctt acaatgcgca
     3721 cgttcagaaa atcatcgatg cccaatgctt caccggcgag ggttgctcgc gaccgcatcg
     3781 cggctgcggc gcgcaggacc gctcaggcag ttgcggtttt cgtcgtgatg ggcgtcttgt
     3841 ttgggaacga agccgcgacg gcacggcctt cgcctatcaa caggatcgta actcaaacag
     3901 ggcccgcgca ttcgtcccgg catgaccggc ggcccgcttc gcccgcccag gcaatcgaac
     3961 gaatttcttc cgaaccgttc gggttgccga cggtcgaagc gctggagggt ccgcttgtaa
     4021 cgaggtggcg cggcgtcgaa agagaaatcc gtcgggatga gctgcttctt gcgcgttgcg
     4081 ccacggatcg gtcatgcggt tcggccccgg ctctacggtt gatggacatc gtcgatgcgg
     4141 cgcgtcgacg cgagggtcgc tcgcgggtcg gggagatcaa ccgggcggtc aatctggcaa
     4201 ttcgtccgat gagcgacgtt gcgcaatacg gagtgcccga cctgtggacc gcgccgattc
     4261 agacgttaac ggccggtgcc ggcgactgtg aagactatgc catcgccaaa tacgtcgcac
     4321 tcgaggaggc cggagtcgcc gaggaggatc ttcgcctagt cgtcgtgcgg gatacgcgac
     4381 ggcgggaaga tcatgccgtg gtcgccgtgc gcttggacgg ccgctggctg gtactggata
     4441 atcgctggct cgccctggtc gaagacgtcg agctcgagac ctaccttcca atgttcggga
     4501 tgaatcgcga gggcgtgaga cgcttcgtcg ccgcccaaca gcgcgtcttt tctcgaggcg
     4561 cgacagagcc ggctccggca cctctgcgct cgcaaaagca gccgctcgag cacgcttcgt
     4621 gactatcgga ctgagaggcg ttggaaatgg caaccccacc cgacaacata atcaattcga
     4681 tgcacgaaac gtttctcctc gtgatcatcg cgttcatcgc cttcgcgctt atcgccgccg
     4741 ccaacggagc gtacacgaac aaggctcgtc acccacgcga ccaagcgagc gacatccggt
     4801 cgggccacga gaccatgatg gctacgccgc
//
LOCUS       DASWGM010000921         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2631353,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000921 DASWGM010000000
VERSION     DASWGM010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2631353"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VGA15_24995"
     CDS             complement(<1..1047)
                     /locus_tag="VGA15_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9590981.1"
                     /translation="MIEISPSIDMLAPIEDRGGAAQPLLQVSGLTKHFPVRGDLFSKR
                     KTVRAVDDVSFAIAKGETVGIVGESGCGKSTTARLLMHLMKRDAGDIIYDGMAVGRAL
                     SLRELRRGMQMVFQDSYASLNPRLTIEESIAFGPKVHGMADVAARALARELLGKVGLR
                     PETFANRYPHEISGGQRQRVNIARALALSPRLVILDEAVSALDKSVEAQVLNLLVDLK
                     REFGLTYLFISHDLNVVRYISDRVLVMYLGEVVELGPVDQVWDAPAHPYTRALLAAMP
                     SSDPDQRTETPPISGDPPNPIDPPSGCRFHTRCPFAEPLCANATPKLTALDRMGHQAA
                     CYMAIAGSGHSLAPS"
     gene            complement(1052..>1562)
                     /locus_tag="VGA15_25000"
     CDS             complement(1052..>1562)
                     /locus_tag="VGA15_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9590982.1"
                     /translation="INTAIMRTVDVFYAFPSVLLAIAISGALGAGIVNSIVSLTVVFV
                     PQITRVAESVTTGVRNMDFVEAARASGAGPFTIMRVHMLGNVLGPIFVYATGLISVSM
                     ILAAGLSFLGLGTKPPEPEWGLMLNTLRSAIYVNPWVAALPGAMIFAVSICFNLLSDG
                     MRSAMDIRN"
BASE COUNT          310 a          493 c          505 g          254 t
ORIGIN      
        1 tgatggcgcg aggctgtggc ctgatcctgc gatggccatg tagcacgcgg cctgatgccc
       61 cattctatcg agcgcggtga gctttggtgt ggcatttgcg cagagcggct ccgcgaacgg
      121 gcaacgggtg tgaaaccggc agccagaggg cggatcgatc ggattgggcg gatcgcccga
      181 aatcggcggg gtttccgtgc gctgatcggg atccgacgat ggcatcgctg ccaataacgc
      241 ccgcgtataa ggatgcgccg gcgcgtccca cacctggtcc accggaccga gctcgaccac
      301 ttcgccgaga tacatcacca gaacccgatc ggaaatgtag cgcaccacgt tgagatcgtg
      361 gctgatgaac agataggtca gcccgaactc gcgcttgagg tcgaccagca ggttgagcac
      421 ctgcgcctcg accgacttgt ccagcgccga gacggcctca tcgaggatca ccagccgcgg
      481 cgacaaggcc agcgcgcggg cgatgttgac gcgctgacgc tggccgccgg aaatctcgtg
      541 cggataacga tttgcaaacg tttccggccg caagcccacc ttgccgagca gctcgcgcgc
      601 cagcgcgcgc gcggccacgt cggccatgcc gtggaccttc gggccgaacg caatcgattc
      661 ctcgatggtc aggcgcggat tgagcgaggc atagctgtcc tgaaacacca tctgcatgcc
      721 gcggcgaagc tcgcgaagcg acaaggcgcg tccgaccgcc atgccgtcat agatgatatc
      781 gccggcatcg cgcttcatca ggtgcatcag aagccgcgcg gtggtcgact tgccgcaacc
      841 ggactcgccg acgatgccga cggtttcgcc cttggcgatg gcgaacgaca cgtcatcgac
      901 cgcgcgcacc gtcttgcgtt tgctgaacag gtcgccgcgc accggaaaat gttttgtcag
      961 cccgctgacc tgcagcagcg gttgcgcggc gccgccgcga tcttcgatcg gcgccagcat
     1021 gtcgatggat ggactgatct caatcatggc gtcagttccg gatgtccatg gcgctgcgca
     1081 tgccatcgct gagcaggttg aagcagatcg acaccgcgaa aatcatggcg ccgggcaacg
     1141 ccgccaccca cggattgacg tagatcgcgg agcgcagcgt gttcagcatc aagccccatt
     1201 ccggctccgg cggttttgtc ccgaggccga ggaacgacag gccggcggcc aagatcatcg
     1261 aaactgaaat gagaccggtt gcgtaaacga aaatcggccc cagcacgttg ccgagcatgt
     1321 gcacgcgcat gatggtgaac ggaccggcgc cggaagcgcg tgccgcctcg acgaaatcca
     1381 tgttgcgcac gccggtggtg acgctctcgg caacgcgggt gatctgcggc acgaacacca
     1441 ccgtcagcga tacgatcgag ttgacgatgc cggcgccgag cgcgcccgaa atcgcgatcg
     1501 ccagcaacac cgagggaaag gcgtagaaca cgtcgacggt gcgcatgatc gcggtgttga
     1561 tc
//
LOCUS       DASWGM010000922         6858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_952236,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000922 DASWGM010000000
VERSION     DASWGM010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952236"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="VGA15_25005"
     CDS             complement(<1..2108)
                     /locus_tag="VGA15_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX9590983.1"
                     /translation="MPKTSEKTLPKPAAVKAPAKGDHVFLVDGSSYIFRAYHALPPLN
                     RKSDGLQVNAVLGFCNMLWKLLRDMPPDNRPTHLAIIFDKSEITFRNKLYPDYKAHRP
                     PAPDDLIPQFPLIREAVRAFDLPCLEQAGFEADDLIATYVRLACERGASATIVSSDKD
                     LMQLVTDCVTMYDTMKDRRIGIAEVIEKFGVPPEKVVEVQALAGDSTDNVPGVPGIGV
                     KTAAQLIVEYGDLETLLLRAGEIKQPKRREALIENAEKARISRQLVLLDDKVKLDVPL
                     DELAVHEPDARKLIAFLKAMEFSSLTRRVAEYSQIDPADIEADAGSKSGASVFSSLPL
                     AEKVDELGNAPDGDLFAGQSAPPRDQVEGKPSPASARGSAPGKTGGGKEDKAGSNKGT
                     PISLATARAEAARKTKIDRSKYQTIRSLEELRWWIARVHDVGHFAIEAKANSVDPMQA
                     EICGIALALGANDAAYVPLGHKQSGDGSGLFAAGLAPEQIKADDALEALRPLLESAGI
                     LKIGFNIKFNAVMFAQAGITIRNHDDAQLMSYALDAGRNSHGLEALAQTWLGHAVLTD
                     SDVIGSGKNKLSIDQVAIDKFTAHAAEDADVILRLWQVLKPRLVAERMTSVYETLERP
                     LISVLARMERRGISIDRQVLSRLSGEFAQTAARVEAEIQEIAGEPINVGSPKQIGDIL
                     FGKMAIPGGSKTKTGAWSTSA"
     gene            complement(2350..3087)
                     /gene="truA"
                     /locus_tag="VGA15_25010"
     CDS             complement(2350..3087)
                     /gene="truA"
                     /locus_tag="VGA15_25010"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX9590984.1"
                     /translation="MPRYKLTIEYDGAPFCGWQIQDNGASVQGALETAIAAICGEQVR
                     VHGAGRTDAGVHALAQVAHCDIAKHFVPGRFRDGLNAHLRPHPIGVLSAEIVPDTFEA
                     RFSAKKRHYLYRISNRRANLALDIGHVWRLPRPLDTEAMQAAAQRLIGKHDFTTFRDT
                     ECQAKSPEKTLDQLDVIRVGDAVNIVTSARSFLHSQVRSMVGSLVWVGEGRWSADDLA
                     AALAARNRAACGPVAPPDGLYLVRVEY"
     gene            complement(3087..4019)
                     /gene="fmt"
                     /locus_tag="VGA15_25015"
     CDS             complement(3087..4019)
                     /gene="fmt"
                     /locus_tag="VGA15_25015"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX9590985.1"
                     /translation="MPLRLIFMGTPDFAVPTLRALEKRGHHIVAVYTRPTKPAKRGLK
                     LQPTPVEIEGRRLGVPVRTPTTLKTWEAFQEFSNYQADAAVVVAYGLILPKEILDAPK
                     FGCFNLHASLLPRWRGAAPIERAIIEGDSETGVMVMKMDAGLDTGDVALAARVQISDT
                     ITGGDLRHVLAEVGAELMAHAMDDLEHHQLKLTRQREIEETYATKIDKTETHIDWDRP
                     ARQVLRHIHGLSPSPGAWCEMPIGNQGARVRILRCELADGEGAPGDLLDDRLTIACGQ
                     GAIRILELQKAGGAPMNAKAFLAGARLRPPARFR"
     gene            complement(4175..4702)
                     /gene="def"
                     /locus_tag="VGA15_25020"
     CDS             complement(4175..4702)
                     /gene="def"
                     /locus_tag="VGA15_25020"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX9590986.1"
                     /translation="MAIREIIILPDKQLRLISKPIEKVTAEVRKLADDMFETMYAAPG
                     IGLAAIQVAQPLRLITMDLAKRDEDGESKPRPRVFINPEILSSSEERSVYEEGCLSIP
                     EYYEEVERPARVRLRFTDLDGKVHEEDAEGLFATCIQHEIDHLNGVLFVDYLSKLKRD
                     RVLKKFAKAAKRAAE"
     gene            4874..6082
                     /gene="rmuC"
                     /locus_tag="VGA15_25025"
     CDS             4874..6082
                     /gene="rmuC"
                     /locus_tag="VGA15_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX9590987.1"
                     /translation="MNEILFVIGDLPVRTGSALIGFGALALLLLLSIAIVIARSGRRG
                     AELAMAQAIRAGELEERLSEMLRAQSEATGRVDAMGQQLAGRQADMARAVNERLDSVT
                     HRVGQSMEQTTRNTMDSLRVLHERLGIIDNAHKNLTDLTSQVTTLRDVLANKQSRGAF
                     GQARMEAIVQDGMPKGSYEFQFTLSTGKRPDCVVFLPDQRPLCIDAKFPLEAMTALHD
                     ARTDEEKKFATQRLRADVMKHVSDIAEKYLITGETQDTALMFVPSESVYAEIHDGFDD
                     VIQKAYRARVVLVSPSLLMLAIQVMQQILKDARMRDAADQIRTEVLNLGDDLGRLRDR
                     VLKLQKHFSDANEDVRQILISADKIEKRAGRIEELDFSKSEAPPEPPRLVKSAAPELF
                     PLPRQLQAGE"
     gene            6575..>6858
                     /locus_tag="VGA15_25030"
     CDS             6575..>6858
                     /locus_tag="VGA15_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturase"
                     /protein_id="HEX9590988.1"
                     /translation="MRQKTELEITLQAVATGEARSGGMEGTEARTATTKVESQAAGGP
                     LMEAIVERDNLRKALAHVKRNKGAAGIDGMSLDALAPHLKEHWPAIRAQLL"
BASE COUNT         1313 a         2100 c         2126 g         1319 t
ORIGIN      
        1 gccgacgtcg accacgcacc ggtcttggtc ttgctgccgc caggtatggc catcttgccg
       61 aacaggatgt cgccgatctg cttggggctg ccgacattga tcggctcgcc ggcgatctcc
      121 tggatctcgg cttcgacgcg cgcggcggtc tgggcgaatt cgcctgatag ccgcgacagc
      181 acttggcggt cgatcgagat gccgcgccgt tccatccgcg ccagcaccga gatcaacggc
      241 cgttccagcg tctcatagac cgacgtcatc cgctcggcga ccaggcgagg ttttaagact
      301 tgccacagcc tcaggatcac gtcggcatcc tcggcggcgt gggcggtaaa cttgtcgata
      361 gcgacttgat cgatactgag cttgttcttg ccgctgccga tgacgtcgct atcggtgaga
      421 acggcgtgac cgagccaagt ctgggccagc gcctcgagcc catgcgagtt gcggccggca
      481 tcgagcgcgt aactcatcag ctgcgcgtcg tcgtggttgc ggatggtaat gccggcttgc
      541 gcgaacatca cggcgttgaa tttgatgttg aagccgatct tcagaattcc cgcggattcc
      601 aatagcggcc gcagcgcctc gagcgcgtcg tccgccttga tttgctcagg tgccaagcct
      661 gcggcaaaca ggcctgagcc atcgcctgac tgcttgtggc cgagggggac ataggcggcg
      721 tcgttggccc ctagcgccag cgcaatgccg cagatctcgg cctgcatcgg atcgaccgaa
      781 ttcgccttgg cctcgatggc gaaatggccc acatcatgca ctcgtgcgat ccaccaccgc
      841 agctcctcga ggctgcggat cgtctggtat ttgctgcgat cgatctttgt cttgcgtgcg
      901 gcttcggcgc gggcggtggc aagcgagatc ggcgtgccct tgttgctccc ggctttgtct
      961 tccttgcctc cgccggtttt gccaggcgcg cttcctctcg cgctggcggg ggagggcttg
     1021 ccttcgactt ggtcacgggg cggggcactt tggcctgcga aaaggtcgcc gtccggcgcg
     1081 ttgccaagct cgtcgacctt ctcggcaagc ggaagcgatg agaaaacact cgcgccactt
     1141 ttcgacccgg catccgcctc gatatccgcc ggatcgatct gcgaatactc cgcgacgcgg
     1201 cgagtcaggc tggaaaattc catcgccttc aaaaacgcga tcagcttgcg cgcgtcgggc
     1261 tcgtgcaccg cgagctcgtc gagcggtacg tcgagcttga ccttgtcgtc gagcagcacc
     1321 agttgccgcg aaatccgcgc cttctcggca ttctcgatca gtgcctcgcg ccgcttcggc
     1381 tgcttgattt caccggcgcg caacagcagc gtttccagat cgccatattc gacgatcaat
     1441 tgcgcggcgg tcttgacgcc gatgccgggc acgcccggca cgttgtcggt ggaatctccg
     1501 gccagcgcct gcacctcgac cactttttcc ggcggcacgc cgaatttctc gatcacctcg
     1561 gcaatgccga tgcggcggtc cttcatggtg tcgtacatgg tgacgcagtc ggtcacgagc
     1621 tgcatcaggt ccttgtcgga cgaaacgatg gtcgcggatg cgccgcgttc gcaggccagc
     1681 cgcacatagg tcgcgatcag atcgtcggcc tcgaagccgg cctgttcgag gcagggcaga
     1741 tcgaaggcgc gaaccgcctc gcggatcagc ggaaattgcg ggatcaggtc gtcgggcgcc
     1801 ggcggccggt gcgccttgta gtcgggatag agcttgttgc ggaaggtaat ttccgacttg
     1861 tcgaagatga tggcgaggtg agtcggccgg ttgtcgggcg gcatgtcgcg caacagcttc
     1921 cacagcatgt tgcagaagcc gagcacggca ttgacctgca agccgtcgga tttgcggttc
     1981 agcggtggca gcgcgtgata ggcgcggaaa atgtaggagg aaccgtcgac cagaaagaca
     2041 tggtcgcctt tcgcaggggc cttgacggca gcgggtttcg gaagagtttt ttctgatgtt
     2101 ttcggcatgg ccgcaactta gggatttttg cggtgattga caccctttgg gacgggatct
     2161 gccctgattt ttacgctccg cgaagcggcc cgaatgggtt gcttcggcgg ccgttccatg
     2221 accccttgcg gtatcaagcg gaccggaagg acgagcccat gaccgcgcaa ctcgacgtgc
     2281 tgatggagaa gctgcgatcg gtctcgcccg gtcaagccgg gcgacgagac atctagacta
     2341 gatgcatcgt caatactcca cccgcaccag atagagcccg tcgggcgggg cgaccgggcc
     2401 gcaggcggcg cggtttcttg cggccaaagc ggccgcaaga tcgtcggcgc tccagcggcc
     2461 ctcgccgacc cacaccagtg agccgaccat cgagcgcacc tggctgtgca ggaacgaacg
     2521 cgccgaggtc acgatgttga cggcgtcgcc cacccggatg acgtcgagct ggtcgagggt
     2581 tttctccggc gatttcgcct ggcattcggt atcgcgaaac gtcgtaaaat cgtgcttgcc
     2641 gatcaagcgc tgtgccgcgg cctgcatcgc ttcggtatcg agcggcctcg gtagtcgcca
     2701 gacatggccg atgtcgagcg cgagattggc gcggcgatta ctgatgcgat agagataatg
     2761 acgctttttc gccgagaacc gcgcctcgaa tgtatccggc acgatctccg cactcagcac
     2821 gccgatcggg tgcggccgca gatgcgcatt caagccatcg cggaaccggc ccggcacaaa
     2881 gtgcttggca atatcgcaat gcgccacctg cgccaatgcg tgcacgccgg catcggtgcg
     2941 gcccgcgccg tgaacgcgaa cctgctcgcc gcagatcgcc gcgatcgcgg tttccagcgc
     3001 accctgcacc gaggcgccat tgtcctggat ctgccagccg cagaacggcg cgccgtcata
     3061 ttcgatggtg agtttgtagc ggggcattag cggaaacgcg ccggaggtcg aagccgagcc
     3121 ccggctagaa aggccttagc gttcattggc gctccgccag ccttctgcag ttcgagaata
     3181 cgaatcgcac cttggccgca ggcgatagtc aatcgatcat ccaacaaatc gccgggagcg
     3241 ccctctccat ctgccaactc acagcgcaag atccttactc tagctccctg attgccgatc
     3301 ggcatttcgc accaggctcc tggagatgga gatagcccgt ggatatgacg aagcacttgg
     3361 cgtgccggcc ggtcccagtc gatgtgagtt tccgttttgt cgattttcgt tgcgtaggtt
     3421 tcctcgattt cgcgttgcct agttaacttc agttggtgat gctccagatc atccatcgca
     3481 tgcgccatga gctcagcacc aacctctgcc aggacatgac gcaagtcacc cccagttatc
     3541 gtatcgctaa tttggaccct ggcagctagc gcaacatcac cggtatcgag gccggcatcc
     3601 atcttcatca ccattacacc agtctcacta tcgccttcta ttatggcgcg ctcgattgga
     3661 gctgcaccgc gccaacgcgg cagcagcgac gcgtgcagat tgaagcaacc gaacttcggc
     3721 gcatcaagaa tttcctttgg cagaattaga ccataggcca caaccaccgc tgcatcggct
     3781 tgatagttgc tgaattcctg aaacgcctcc catgtcttca gcgttgttgg tgtacgcacg
     3841 ggcacaccca gccttctgcc ctcgatctcg accggtgtgg gctgaagctt caatcctctc
     3901 ttagctggct ttgtcggacg cgtgtaaact gccactatat gatggccgcg cttttcgagc
     3961 gcacggagcg tcggcaccgc gaaatccggc gtacccatga agatcagacg aagaggcatc
     4021 gtaataactc ggtgcgttcc ctcccccctt gtgggggagg gctagggaga ggggtaagca
     4081 acaagcaccg agcccgcggc acccccctct ccacctctcc cccacaaggg gggagagagc
     4141 agatcgagcg aggtgcgacc gccatgcctc tcacctactc cgccgcgcgc ttggcggctt
     4201 tggcaaactt cttcagcacg cggtcgcgct tcagttttga tagataatcc acgaacagca
     4261 cgccattgag atgatcgatt tcgtgctgga tgcaggtggc gaacaggcct tcggcgtctt
     4321 cctcgtgcac cttgccgtcg agatcggtga aacgcaggcg cacccgcgcc ggccgctcca
     4381 cctcctcgta atactccgga atcgacaggc agccttcctc gtaaaccgag cgttcttccg
     4441 acgacgacag aatttccgga ttgatgaaga cgcggggccg cggcttgctc tcgccgtctt
     4501 cgtcccgctt ggcgaggtcc atggtaatca gccgcagcgg ctgcgcgacc tggatcgcgg
     4561 cgagcccgat ccccggtgca gcgtacatgg tctcgaacat gtcgtcggcg agcttgcgga
     4621 cctccgccgt gactttttcg atcggtttgg agatgagccg caactgtttg tcgggcagga
     4681 tgatgatttc acgaatggcc atggcgggcg atttaagctg ctgatttgcc ggggtcaatc
     4741 cgcgccgctt gctgttaacg cgttgttagc cacgaatctt aggccggcgt taaccacgaa
     4801 aattaataca tcatttacca aaaatgttcc ctcttcgttc gccttgacgc ccgaatcggc
     4861 tacaagggct ctcatgaacg agattctttt tgtgatcggt gacctgccgg tacgcaccgg
     4921 tagcgcgctg atcggtttcg gcgcgctggc gctgctgttg ctgctgagca ttgccatcgt
     4981 gattgcgagg tccggccggc gcggcgcgga attggcgatg gcgcaggcta tccgcgccgg
     5041 cgaactggaa gaacgcctga gcgaaatgct ccgcgcccag agcgaggcca ccggccgcgt
     5101 cgatgcgatg gggcagcaac tggccggccg ccaggccgac atggcccgcg ccgtcaacga
     5161 gcggctggat tcggtgaccc atcgtgtcgg ccaatcgatg gagcagacca cccgcaacac
     5221 catggacagc ttgcgcgtgc tgcacgaacg gctcggcatc atcgacaatg cccacaaaaa
     5281 cctcaccgac cttacctccc aggtgaccac gctgcgcgac gtgctggcca acaagcaatc
     5341 gcgcggcgcg ttcggccagg cgcggatgga agccatcgtc caggacggca tgccaaaagg
     5401 cagttacgaa ttccagttca cgctctccac cggcaagcga ccggattgtg tggtgtttct
     5461 gcccgatcag cgcccgctct gcatcgatgc gaaatttccg ctcgaggcga tgaccgcgct
     5521 gcatgacgcg cgcaccgacg aggaaaagaa attcgccacc caacggctgc gcgccgacgt
     5581 gatgaagcac gtcagcgata tcgccgaaaa atatttgata acaggcgaaa cccaggatac
     5641 cgcgctgatg ttcgtgccgt cggaatcggt ctacgccgag atccatgacg gctttgacga
     5701 cgtgatccag aaggcctatc gcgcccgggt ggtgctggtg tcgccgtcgc tgttgatgct
     5761 ggcgatccag gtgatgcagc agatcctgaa agacgcgcgg atgcgcgacg ccgccgacca
     5821 gatccgcacc gaagtgctca accttggcga cgatctcgga cgcctgcgcg atcgcgtgct
     5881 aaaactgcag aagcattttt ccgacgccaa cgaggatgtc cggcagatcc tgatttccgc
     5941 cgacaagatc gaaaagcgcg ccgggcggat cgaggaactc gatttcagca agagcgaagc
     6001 gcccccggaa cccccgcgcc ttgtcaaatc agccgcgcct gagctgtttc cgctgccgcg
     6061 ccagctgcag gcgggggagt agcttttctc gtgcagtgaa gcacgagagg cgacacacaa
     6121 actgcgtcat cgcccggctt gaccgggcga cccagtagtg tgccgagcat ggtcgacagc
     6181 ttggaggtga aagtcttcta tccagcctga tggtggcgaa ggattagcga agcgcaaggg
     6241 cgtcgtcgcg aggcggggtc tgaaggaagc gtggagcaaa gccgcggccc gatggacaaa
     6301 caccggataa agaggcctac ccggccggac gagcgagcta ctaatcgcga agtccacaac
     6361 catcaaaggc cggggtggta aattcggcgg gcgtgcggcg aaggcggtcg atcttacctc
     6421 gggagatctg cgctgtgtcc ctgagtcggg actgaggtcg ccgcaaggcg gcctgaccgc
     6481 agcgcagaag tcagcagaag gcgtagtagg tggggcgacc tgccaaaggc ctgaacgacg
     6541 gaatggcaag tagaacggcg atctcggacg ggtgatgcgg cagaaaactg aactggagat
     6601 caccttgcag gcggtggcga cgggtgaagc ccgaagcggc ggcatggaag ggaccgaagc
     6661 ccggacggcg acaaccaaag tcgaaagcca ggcggccggt gggccgttga tggaagcgat
     6721 tgtcgagcga gacaatctga ggaaagcgtt agcgcatgtg aaacgcaaca aaggagctgc
     6781 cggtatcgat ggcatgagcc tcgatgccct ggctcctcac ctgaaagagc actggcccgc
     6841 gatccgggcc cagctgct
//
LOCUS       DASWGM010000923         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1207728,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000923 DASWGM010000000
VERSION     DASWGM010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207728"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VGA15_25035"
     CDS             complement(<1..479)
                     /locus_tag="VGA15_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX9590989.1"
                     /translation="MAAALAASPARAQTRVGEAAVVKNEVVRVMASATSQINVGDGVL
                     RDEIVRTGLDSAARLVMADSTNLSLGPSATITLDRTVFNDEHSYRDITIRLTTGAFRF
                     VTGHSDKNAYKITTGLATIGVRGTTLDILSQRGKTTVKLQQGAATVCTVSFQCIELT"
     gene            729..1526
                     /locus_tag="VGA15_25040"
     CDS             729..1526
                     /locus_tag="VGA15_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX9590990.1"
                     /translation="MSLVRKSPSVFDNSAAWSVIVVAAVFVVARLALLVGVTTPEKFH
                     FDEVHRVPAARQMLEPVMPSPLLNPTHPPRANELMAVSIRVFGDAPLGWRNPSVPFGS
                     LAIVAMYLCGLALFTAQGPAVASALIAFFNQMVFVQSRIAMPDISALAFGLLAIAAFI
                     SGFRKRRPHLWFALAGLAFGLSIACKWRGLFALAICIVIVAVIRLMQSWQFADYALRR
                     GNGPRWLLWAFIAIAFAGFAAMPPISAAFVGTSMETFDRLMIFQNWI"
     gene            1665..>3263
                     /locus_tag="VGA15_25045"
     CDS             1665..>3263
                     /locus_tag="VGA15_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9590991.1"
                     /translation="MWAFFERLLDSSMFSPHGICLLWEPELIWLHVTSDAIIAVAYFS
                     IPVALSIFVSKRRDVDFGWIFWAFAIFIMACGFTHVLSIVTLWVPVYGIEGIIKALTA
                     AASIVTAVMLWPLLPKVLALPSPSQLRQAEAALAQEGLHRREAEDMLRQSQKMEAMGQ
                     LTGGVAHDFNNLLTIISGNLEIAETSLQSWGDATRELLTRVIANAAHGAQRAAMLTQR
                     LLAFARRQPLDPRLTDVNQLLAGMSDFFRRTVGENIDLEVVGGADLWQVEVDPGEMEA
                     SILNLVVNAKDAMAGKGKLTIETSNAFIDENYSQQNADVPVGQYVQLAVTDNGSGMSP
                     DVQEKAFDPFFTSKQPGQGTGLGLSQVYGFVKQSGGHVKIYSEVGEGTTIKIYLPRSD
                     VAAGAVRESNLPVVGSSGSETILVVEDEADVRSYLVETLEDLNYRVREAPDGATALAQ
                     FDSDPFRIDLLLTDIVMPGINGRQLADELHYRQPDLNVLFMTGYARNAIMHQGRLDSG
                     VSLLQKPLTQVVLATKIRDILDRPQ"
BASE COUNT          593 a          966 c         1002 g          702 t
ORIGIN      
        1 tgggtcagtt cgatgcactg aaagctgacg gtgcaaaccg tcgccgcacc ctgctgaagc
       61 ttgacggtgg tcttgccccg ctgcgacagg atatcgagcg tggtgccgcg cacgccgatc
      121 gtcgccagtc ccgtggtgat cttgtaggcg ttcttgtcgg agtgtccggt cacgaaacgg
      181 aatgcgccgg tggtcaggcg gatcgtgatg tcgcggtaac tgtgctcgtc attgaagacg
      241 gtgcggtcca gcgtgatggt cgcgctcggc ccgagtgaaa gattcgtgct gtcggccatc
      301 accagccgcg ctgcgctgtc gaggccggtg cgcacgatct cgtcgcgcag cacgccgtcg
      361 ccaacgttga tctggctggt tgccgacgcc atcacgcgga cgacttcgtt cttgacgaca
      421 gccgcttcgc cgacgcgcgt ctgcgcgcga gcgggactcg ccgcaagggc tgctgccatg
      481 cataacgcga aaatacaaag aacgccgtga cgcgaattca tttcaaatac cgattcaata
      541 ccgatcgatt tattcgccca tggtaccgga ccgcgcaggc ggccgatatt gtaaatttgc
      601 cacaagcggc ccataaccat cccattggtt ggtgcacgcg aaatcgaaat gcgttcaatg
      661 caaatggatt taaaaagcgg tctctcctcc attcgaatga cacgcgatcg ttgcagcgtc
      721 attgatttgt gagcctcgtg cgaaaatcac cgtctgtttt cgacaattcg gctgcctgga
      781 gcgtcattgt tgtcgctgct gtctttgtag ttgcacgcct cgcgcttctg gtcggcgtga
      841 cgacgccgga aaaattccat ttcgatgaag tgcatcgtgt gccggccgca cggcagatgc
      901 ttgagccggt catgccgagc ccgctgctca acccgacgca tccgccgcgg gcgaacgagt
      961 tgatggcggt atcgattcgg gtgttcggag atgcgccgct gggctggcgc aatccgagcg
     1021 tgccgttcgg atcgctcgcg attgtcgcga tgtatctgtg cggcctcgcg ctgttcaccg
     1081 cgcaaggccc tgcggtcgca tctgcgttga tcgccttctt caaccagatg gtgttcgtgc
     1141 agtcgcggat cgcgatgccg gatatttccg cgctcgcgtt cggcctgttg gcgatcgcag
     1201 cgttcatctc cggctttcgg aaacggcggc cgcatctctg gtttgctctc gcagggctag
     1261 ctttcggcct ttcgatcgcc tgcaaatgga gaggactgtt tgcactggcg atctgtatcg
     1321 tcatcgtcgc cgtgatccgc ctgatgcaaa gctggcagtt tgccgactat gcgctgaggc
     1381 gcggcaacgg cccgcgctgg ctgttatggg ctttcatcgc gatcgccttc gcaggttttg
     1441 cggcgatgcc gccgatctcg gctgcgttcg tgggaacgtc gatggagacc ttcgaccggc
     1501 tgatgatttt tcagaactgg atttaaggga ttccgcgcgc cgaggcgaag cctctctgat
     1561 ctgatcggcc cgcatcggga tcacttgacg ggcctcgcgc tgctcgtcaa tttggctcct
     1621 caagagcctt tccgatcacc cggttcgaac ctggaacgag catcatgtgg gcttttttcg
     1681 agcggttact cgattcgtcg atgttttccc cgcatggaat ttgcctgctg tgggaaccgg
     1741 aactgatctg gctccatgtc acgtccgacg cgatcatcgc cgtcgcctat ttctcgattc
     1801 cggttgcgct ttcgattttc gtatcgaagc ggcgcgacgt cgatttcggc tggattttct
     1861 gggcctttgc catcttcatc atggcctgcg gctttaccca cgtgctgtcg atcgtgacgc
     1921 tgtgggtacc ggtctatggc atcgagggga tcatcaaggc gctgacggcc gcggcgtcga
     1981 tcgtcaccgc ggtgatgctc tggccattgc tgcctaaagt tcttgcactg ccgtcgccgt
     2041 cgcagcttcg ccaggccgag gccgcactgg cgcaggaggg tctgcaccgg cgcgaagccg
     2101 aggacatgct ccggcaaagc caaaaaatgg aagcgatggg gcagttgacc ggcggcgtcg
     2161 cgcatgactt caacaatctg ctcacgatca tcagcggcaa cctcgaaatc gccgagacca
     2221 gcctgcagtc ctggggcgat gcaacgcggg aacttttgac gcgcgtgatc gccaatgccg
     2281 cgcacggggc gcagcgtgcg gcgatgctga cgcaacggct gctcgccttc gcgcgccggc
     2341 agccgctcga tcccaggctg accgacgtca atcagctgct cgccggaatg tctgattttt
     2401 tcaggcggac ggtgggtgaa aatatcgatc tcgaagtcgt aggcggcgcc gacctctggc
     2461 aggtcgaggt cgaccctggg gagatggagg cctcgatcct caaccttgtg gtgaacgcca
     2521 aggacgccat ggccggcaag ggcaaactga ccatcgaaac cagcaacgcg tttatcgacg
     2581 agaattactc gcaacagaac gccgacgttc cggttgggca atatgttcag ctcgcggtga
     2641 ccgacaatgg ctccggaatg tcgccggatg tccaggagaa ggccttcgat ccgtttttta
     2701 ccagcaagca gccggggcag ggcaccggcc tcggattgag ccaggtgtat ggcttcgtca
     2761 aacaatccgg cggccacgtc aaaatctaca gcgaggtcgg cgaagggacg acgatcaaga
     2821 tctatctgcc tcgatcggac gtcgccgccg gcgccgtcag ggaaagcaat ctgccggtgg
     2881 tcggcagcag cggaagcgaa accatattgg tggtcgagga cgaagccgac gtcagatcct
     2941 atctggtgga aacgctggag gatctgaact accgtgtccg tgaggcgccc gacggcgcga
     3001 ccgcgctggc gcagttcgat tccgatccgt tccggatcga tctgcttctc accgacatcg
     3061 tgatgcccgg catcaacggc cgtcaactgg cggacgagct gcactaccgc cagccggacc
     3121 tcaatgtgct tttcatgacg ggatatgcgc gcaatgccat catgcatcag ggccggctgg
     3181 attccggcgt ctcgctgctt cagaagcccc tgacccaggt cgtgctggca acaaaaatca
     3241 gggacattct cgacaggccg caa
//
LOCUS       DASWGM010000924         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1073211,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000924 DASWGM010000000
VERSION     DASWGM010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073211"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VGA15_25050"
     CDS             complement(<1..176)
                     /locus_tag="VGA15_25050"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045638.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX9590992.1"
                     /translation="MNAPIPATEDLIYVVEDGIARLTFNRPQARNALTFAMYEQMAAI
                     CETVNADRSIKAMIL"
     gene            complement(224..1429)
                     /locus_tag="VGA15_25055"
     CDS             complement(224..1429)
                     /locus_tag="VGA15_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX9590993.1"
                     /translation="MTLHTGRHFLQIPGPTNVPDRVLRAMDMPTLDHRGPEFAELGHA
                     VLAGCQRIFRTQQPVIIYPASGSGAWEAAIVNTLSPGDKVLMAETGQFAVLWLGIAEK
                     FKLDVEFLPTDWRRGADVEAIEAKLAADRLHKIKAVMVVHNETSTSCVTHPLEVRKAL
                     NRTNHPALLMVDTISGLASLEYEHDAWGIDISVGGSQKGMMLPPGLSFNAISEKALAA
                     SRANTSLRSYWDWDDMIAINKLGTFPYTPATNLLFALKEAIAMLEEEGLDNVFARHQR
                     HGAATRAAVKAWGLDLVCLDPHAYSPALTAVMVPDGHDADALRKVILENFDMSLGTGL
                     GKLKGKAFRIGHIGHFNDLMLMGTLSGVEMGLDLAKVPHRNGGVLAAMDVLKRRDVVS
                     MPKSKAAVA"
     gene            complement(1631..>2528)
                     /locus_tag="VGA15_25060"
     CDS             complement(1631..>2528)
                     /locus_tag="VGA15_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9590994.1"
                     /translation="KRGFAQGITHAFARLGNAITPPLIAALMFWLTWRGSFIALGLVS
                     LLWGVVWAAYFRNEPKDHPGITEAELASLPPRPVNMRPQVPWGALLRRMWPVTLTYFC
                     YGWSLWLYLNWLPLFFKNNYSLDIKNSALFASGVFFAGVVGDSLGGILSDRILKKTGN
                     VRFARLSVTITGFAGALLSLFPILFIHDITIVALCLSAGFFFAELVIGPMWSVPMDIA
                     PKYSGTAAGLMNTGSALAAIVSPLVAGYVIDVTGNWYLPFLMSMGLLLIGGFSAFLMH
                     PETPFEEEGGVIPMAVRPAAAG"
BASE COUNT          498 a          788 c          769 g          473 t
ORIGIN      
        1 aggatcatcg ccttgatcga gcggtcggcg ttgacggtct cgcagatcgc cgccatctgc
       61 tcgtacatcg caaacgtcag tgcgttgcgg gcttgcggcc ggttgaaggt cagccgggcg
      121 atgccgtcct cgacgacata gatcaggtct tcggtcgctg gtatcggggc gttcatggta
      181 cgctctttct gttcttgccg ttgttgcgca gcattgaaac aattcaggcg acggcagcct
      241 ttgatttcgg catcgacacc acgtcgcgtc gtttcaggac atccatggcg gcgagcacgc
      301 caccgttgcg atgcggtacc tttgcaagat ccaggcccat ctcgacaccc gacagcgtcc
      361 ccatcagcat cagatcgttg aaatgtccga tatggccgat ccggaacgcc ttgcccttga
      421 gctttccaag gccggtgccc agcgacatgt cgaagttttc caggatcacc ttgcgcaagg
      481 cgtctgcatc gtgtccatcc ggaaccatga ccgcggtcag tgccggcgaa taagcgtgcg
      541 gatcgaggca cacgaggtcc agaccccatg ccttgaccgc cgcgcgggtc gccgcgccat
      601 ggcgctggtg tcgcgcgaac acgttatcca gcccctcttc ctccagcatc gcaatggctt
      661 ccttgagcgc aaacagcagg tttgtcgctg gcgtgtaggg aaatgtgccg agcttgttga
      721 tcgcaatcat gtcgtcccag tcccagtagg accgcaacga cgtgttggcc ctggaggccg
      781 cgagcgcttt ttccgagatc gcattgaagc ttagccccgg cggcagcatc atgccctttt
      841 gcgacccgcc gacggatatg tcgatgcccc aggcgtcgtg ttcgtactcg agcgaggcga
      901 gcccggagat ggtgtcgacc atcagcagcg ccgggtggtt ggtgcggttg agggctttgc
      961 gaacttcgag cggatgcgtg acgcaactgg tcgaggtctc gttgtgcacg accatcacgg
     1021 ccttgatctt gtgcaggcga tcggcggcaa gcttcgcctc aatcgcttcc acatcggcgc
     1081 cgcgccgcca gtcggtgggc aagaattcga catcgagctt gaatttctcg gcaatgccga
     1141 gccacaacac cgcaaactgg ccggtctcgg ccatcagcac cttgtcgccg ggcgacagcg
     1201 tgttcacgat cgcggcttcc caggcgccgc tgcccgacgc cggatagatg ataaccggct
     1261 gctgggtccg gaaaatgcgc tggcaacctg cgagcacggc atgaccgagt tcggcgaatt
     1321 cagggccgcg atgatccagg gtcggcatgt ccatggcccg cagcacccgg tcgggcacgt
     1381 tggtcggccc tggaatctgt aaaaaatgcc ttcccgtgtg caaggtcatc tggcgtcctt
     1441 cccggtcttg tctttggttc gcgtctcttg gttcgcggcg ctcgaatatc actattcggc
     1501 gggcttgtcc cctgcccggc gggcgccgtc aatgcataag aagccatgct ttgcctcgca
     1561 aagcacaaga aacggggctt tgccgtgcaa agccgaaccc cgggggatcc ctgacatgcg
     1621 gggggctggt ttatcccgcc gcggctggtc gtaccgccat gggaatcacg ccgccttctt
     1681 cctcgaacgg cgtctccgga tgcatcagga acgccgaaaa ccctccgatc agcaatagtc
     1741 ccatcgacat caggaacggc agataccagt taccggtcac atcgatcacg taaccggcca
     1801 ccaaaggcga aacaatcgcc gccagcgccg agccggtatt catcaggcca gcggcggtac
     1861 cggaatattt cggcgcgatg tccattggca ccgaccacat cgggccgatg acgagttcgg
     1921 caaagaagaa gcccgccgac aagcacaagg ctacgatggt gatatcgtga atgaacagga
     1981 tcgggaacag cgacagcaag gcgccggcga agcccgtgat cgtcacgctc agccgcgcaa
     2041 agcggacatt gccggtcttc ttcagaatcc gatcggacaa aattcccccc aggctgtcgc
     2101 cgacgacgcc ggcaaagaac acgcccgagg cgaacagcgc cgaattcttg atatccaggc
     2161 tgtagttgtt cttgaagaac agcggcagcc agttcaggta cagccacagg ctccagccgt
     2221 agcagaaata ggtcagcgtg accggccaca ttcggcgcaa cagcgccccc cagggtacct
     2281 gaggccgcat attgacgggg cgtggcggaa gcgatgccag ttcagcctcg gtaatcccgg
     2341 gatgatcctt gggctcgttg cggaaatagg cggcccagac cacgccccac agcaggctga
     2401 ccaatccgag cgcgatgaag gagccgcgcc aggtcagcca gaacatcagc gcggcaatca
     2461 acggcggcgt gatcgcgttg cccagccggg cgaaggcgtg ggtgatgccc tgggcgaagc
     2521 cgcgcttg
//
LOCUS       DASWGM010000925        10464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_390880,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000925 DASWGM010000000
VERSION     DASWGM010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390880"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGA15_25065"
     CDS             <1..274
                     /locus_tag="VGA15_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sorbitol dehydrogenase"
                     /protein_id="HEX9590995.1"
                     /translation="MGQFTDAGSIETSWHRICTKDLNVLGSWGFTGNDLPLGVDMLYR
                     TREKYPWLEMQAIYPFTEEGIGRAVADAMAMKTVKSTIVPWPELAE"
     gene            complement(321..1847)
                     /locus_tag="VGA15_25070"
     CDS             complement(321..1847)
                     /locus_tag="VGA15_25070"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX9590996.1"
                     /translation="MNEARRAFANGDLATAERICSQVLAHAPDDGAVWALLTETALQR
                     GRPDAAIVCADRAVALMPKDPLALVLRAKCLFVSGEARGAFEAAEAASKMIDDAPETL
                     DACGAIFGLLGLHQRAKEFFLRAVAARPDAPQYLFNLAATERMTGALDEAEAHCDAAI
                     ALDRRYGLAHYLRSDLRIQTADRNHIEEMEALLRDGKLALPSEVILRFALGKEYEDLD
                     AHDRAFDHVDAGCNLQRRSITHDRAAEIAEVDRIIQTHTRSWIDAAPAGYCDAAPVFV
                     AGLPRTGTTLVERIIASHSAMSSVGETGAFATEMRRAMKGMPTAGDLADIGRRYIDSV
                     TAFRKLRSQRFVDKTLQNYLYCGLVHAALPAARIILVQRHPLDACWAIYKAHFQGKFS
                     FSYHQIELAEYYLAYRRVSRHWRATLPSDVLLEINYEDIVRDQSAASRRLIGFVGLPW
                     DDEVLRFHQSPAPSATASAVQVRRPIYSSSVGKWRHHAERLAPLRARLAREIPEAELA
                     "
     gene            complement(1995..4076)
                     /locus_tag="VGA15_25075"
     CDS             complement(1995..4076)
                     /locus_tag="VGA15_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9590997.1"
                     /translation="MLKQLTKRLRDRTVITAAILSLMGCDAYAVENTAMGGTHTGDRR
                     TASPIKHVIVIIGENRTFDHVFATYEPRDGEHVDNLLSKGIIRKDGSPGPNYAKAAQF
                     STTVNNFFSIDPTPKTAYNTTTNKLQAPGTSYAPQNCYATVEQAALNGPGCLATLAEA
                     ARGDYGLRKQDLPLLTTGATGIPGNSPDARILDSSNLPNGPYPLVTLPQGEDETSSLY
                     KTYGGSPVHRFYQMWQQLDCDVAKATQRNPSGCQADLFPWVEETVSSGSNGNPTPKPL
                     KEGDIAMGFYNVANGDAPYLTKLAREYTLNDNYHQAAMGGTYANHMMIGYADELYYAD
                     QNGDPATPNHVLVPGSNPPVFVDEVENPNPAPGTNNWYANNGYGGGSYTNCSDFTQPG
                     VAPIVNYLNSIHVPPNCAPRAYYLLNNYVPSYIGSGGTDPINNGPFTLPPVIKQKHIG
                     DALSQAQVSWAYFGERWNDFKTAPGEGANFGSLDPIAYLYCNICNPFLYSASVMTSQT
                     QRDAHLKDTLDLYDAIEDGTLPAVSFVKPSTFNDGHPSSSRLDLFESFTRRIIDQVKS
                     HEELWESTAILVTVDEGGGYYDSGYVQPLDYFGDGTRIPLIVVSKYSQGGRVSHEYSD
                     HASIPKFIERNWDLPKLSTYSRDNLPNPKTSHDNPYVPQNRPALGDLFGNFQFADHDG
                     GDHDGHHDGDH"
     gene            complement(5122..5679)
                     /locus_tag="VGA15_25080"
     CDS             complement(5122..5679)
                     /locus_tag="VGA15_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9590998.1"
                     /translation="MKHPSSREFFAYWDEQRGNARAPERSEIEPGAVRELLGDIFVLS
                     YDAAAGYPFRVAGTRVCALLGCDLKDRSFSALFEPEGRREIEDIIAVVSEEMLAAVAG
                     ITATSQDGSRAHLELLLLPFNTRAHTPLTLTGLLAPFGNNHSVLRDFQLTSWRYLGHQ
                     PQKLLPRALRKLAVARGFMVYEGLR"
     gene            6170..6631
                     /locus_tag="VGA15_25085"
     CDS             6170..6631
                     /locus_tag="VGA15_25085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9590999.1"
                     /translation="MRGVSFMTSNETMSAVRALLESYRDAFERLDAAAIADHFVYPSH
                     ITGDADEIVLLQLSNRQDCLAAVEKVLAMHRELEAPTGSICDLSIIELSPRLTRASFR
                     MEVHRRTGGMLYDFEAAYTLAETRAGWRIAAIAHNQIPRLLKCLAQRQAGA"
     gene            complement(6653..7930)
                     /locus_tag="VGA15_25090"
     CDS             complement(6653..7930)
                     /locus_tag="VGA15_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9591000.1"
                     /translation="MTALTYREKFYGWRIVGAAFVLAVFGWGLGFYGPPIYLQAVRDL
                     RGWSLPLVSAAVTVHFLFGALVVARLPKLYLRYGLPAVTKAGVLSLALGISGWAVAAE
                     PWQLFAAALFSGAGWVAMGAAAVNAILAPWFVRTRPAALAMAYNGASIGGVVFSPLWV
                     AAIGMWGFPAATAVVGVIVTICVWILADVYFCRTPQQMGLAPDGDEPGVAAVFGSFAA
                     ARPLSGASLWKDRRFVTLAAGTALGLFAQIGLLAHLFSLLVPAVGAQLAGIAISAATM
                     AAIAGRTLVGWLMPVGADRRLVACGSFTVQITGSIAFLIAGGTHIPLLVLGVILFGVG
                     IGNATSLPPLIAQAEFAKQDVLRVVSLNVAISQGTYAFAPASFGLIREFAPQAGVLAG
                     AAAPYLFAAAALLQALALTAFLAGRRRSTACSF"
     gene            8100..8810
                     /locus_tag="VGA15_25095"
     CDS             8100..8810
                     /locus_tag="VGA15_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX9591001.1"
                     /translation="MASTAAFAEVAAMAGDPARAAMLHALMDGRALTASELARVAGIA
                     PQTASGHLARMTQTGLLAVARQGRHRYHRLATSGVARMIEGIMRVASELQPPRKNLSV
                     GPRDVALRQARTCYDHLAGRLGVSLSDALTSAGYAELSAESGIITERGLAFLERVGID
                     VSAMRARGGMKSGRLLCRPCLDWSERRSHLAGVVGAALCAHSFAQGFVCRIDGTRALR
                     ITPKGLRVYRETFGVRFE"
     gene            complement(9091..10320)
                     /locus_tag="VGA15_25100"
     CDS             complement(9091..10320)
                     /locus_tag="VGA15_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591002.1"
                     /translation="MPAFRDRLQVLSATIIVLAATSTGAIAQKKYDIGATDTEIKIGN
                     IMPYSGPASAYGVIGKTEAAYFKKINAEGGINGRKIKFISYDDAYSPPKAVEQARKLV
                     ESDEVLFIFNALGTPSNSAIQKYMNEKKVPQLFVQTGATKWNDSKQFPWTMGWPPNYQ
                     SEGRIYARYILKERPTGKIGILYQNDDYGKDYIKGITDGLGTKAASMIVGEESYETTT
                     PTIDSHVVALKSEGADIFISITTAKFAAQSIKKVAELGWKPLFILNGPGASTGTVMKP
                     AGLENSQNIISATYQKDPTDPQWNDDAGIKNFDTFLTKYFPEGNREDLNLMTGYNVAQ
                     TMVYVLKKCGDDLTRANIMKQAASIKSLQLEGLLPGVTINTSATDFAPIKQFQLRKFK
                     GERWELFGDVISSEVGE"
BASE COUNT         2014 a         3069 c         3241 g         2140 t
ORIGIN      
        1 gatgggccag ttcaccgatg ccggttcgat cgagacgtca tggcatcgca tctgcaccaa
       61 ggacctcaac gttctgggct catggggttt taccggcaac gacctgccgc tcggcgtcga
      121 catgctctat cgcacccgcg aaaaatatcc ctggctcgag atgcaggcga tctatccctt
      181 caccgaggaa ggcatcggcc gcgcggtcgc ggatgcgatg gcgatgaaga cggtgaaatc
      241 gaccatcgtg ccgtggccgg agctggcaga gtgattttgg ggcgtggacg aatctcaagc
      301 ccgattgccg tagggcggcc tcacgccagt tccgcttccg gaatctcgcg cgccagccgc
      361 gcgcgcaacg gcgcgagccg ttccgcatgg tgtcgccatt tgcccactga ggacgaatag
      421 atcgggcgcc gcacctggac ggcgcttgcg gtcgcagacg gcgccgggct ctggtgaaat
      481 cgcaggacct cgtcgtccca cggcagtccg acaaagccga tgagacgtcg acttgctgcc
      541 gattgatctc tcacaatgtc ctcgtaattt atttccagca acacatccga cggcagtgtc
      601 gcccgccaat gccgcgagac gcggcgatag gccagataat attccgccaa ctcgatctga
      661 tgatacgaga acgaaaactt gccttgaaag tgagctttgt agattgccca gcacgcgtcc
      721 agtggatgtc gctgcaccag tatgatcctg gccgccggca gcgccgcgtg aacgaggcca
      781 cagtagaggt agttctgcag cgtcttgtcg acgaaacgct gacttcgcag cttgcggaac
      841 gccgtcaccg aatcgatata gcgtctgccg atatccgcaa ggtcgcctgc tgtcggcata
      901 cccttcatgg cgcggcgcat ctcggtcgca aacgccccgg tttctccgac cgagctcatc
      961 gccgagtggc tggcgatgat ccgctcaacc aatgtggtgc cggtgcgcgg cagcccggcg
     1021 acgaacacgg gtgcggcgtc gcaataaccg gccggggcgg cgtcaatcca gcttcttgtg
     1081 tgtgtctgga tgatccgatc gacctccgcg atctcggcgg cacgatcatg cgttatcgag
     1141 cggcgctgca gattgcagcc ggcatcgaca tgatcaaacg cgcggtcgtg tgcatcgagg
     1201 tcttcgtatt ccttgccgag cgcgaagcgc agtatgacct cgctcggcaa cgccaacttg
     1261 ccatcgcgga gcagcgcttc catttcctcg atatgattgc gatcggcggt ctggatacgc
     1321 aggtctgagc gcaggtagtg ggccaggcca tagcggcggt cgagtgcgat ggcggcgtcg
     1381 caatgcgctt cggcctcatc caacgccccg gtcattcgct cggtcgccgc gagattgaac
     1441 aggtactgcg gcgcatcggg acgggcggcg accgcgcgca ggaagaattc cttcgccctt
     1501 tgatgcaagc cgagcaggcc gaagatcgcc ccacacgcgt cgagcgtttc gggggcgtcg
     1561 tcgatcatct tcgaagcggc ttcggcggcc tcgaacgcgc cgcgcgcctc accggaaaca
     1621 aacaggcatt ttgcgcgcag aaccagcgcg agcggatcct tcggcatgag ggcgaccgcg
     1681 cgatcggcgc agacgatggc cgcatcgggg cgcccccgct gcaaggcggt ttctgtcaga
     1741 agtgcccaaa cggctccatc gtccggcgca tgtgcgagta cctgggagca aatccgttcc
     1801 gccgtcgcca gatcgccatt ggcgaatgcg cgtcgggcct cgttcacggg cggtacgttg
     1861 gctcgctgct gagctgtggt aggcaagcgg tcgctccatc atggccgggc gagatacgcc
     1921 cggccatctg cgcttatgtg aatggcatcg gtcagcgaac ggctgcttcc gtcgccggaa
     1981 gcagccattg ccgatcaatg gtcgccgtcg tggtggccgt catgatcccc gccatcgtga
     2041 tcggcgaact ggaaattgcc aaagagatcg ccgagcgccg gccgattctg cggaacgtac
     2101 gggttgtcat gcgacgtctt cgggttcggc agattgtcac ggctgtaggt ggagagtttc
     2161 ggaagatccc agttccgttc gatgaatttc gggatcgagg catgatcgga atattcatgc
     2221 gagacgcgcc cgccttgcga atatttcgac acgacaatga gcggtatgcg ggttccatcc
     2281 ccgaaatagt cgagcggctg gacgtaaccg gaatcataat agccgccgcc ctcatcgacg
     2341 gtgaccagga tcgcggtgga ttcccagagc tcctcgtggg acttcacctg gtcgatgatc
     2401 cgccgcgtga aggattcgaa cagatcgagc cgcgacgagg acggatggcc atcgttgaag
     2461 gtgctcggct tcacgaacga cacggccggc agagtaccgt cttcgatggc atcatagaga
     2521 tcgagcgtat ccttcaggtg cgcatcgcgc tgtgtttggc tcgtcatgac cgaagcggaa
     2581 tagaggaacg gattgcagat gttgcaatac agataagcga tcggatcgag agatccgaag
     2641 tttgcgccct caccaggggc ggtcttgaag tcgttccaac gctcgccgaa atacgcccac
     2701 gacacttggg cctggctgag cgcatcgccg atgtgcttct gcttgatcac cggcggcaac
     2761 gtgaatgggc cattattgat cggatcggtg ccgccgctgc cgatgtaaga cgggacatag
     2821 ttatttagca gataataggc gcgcggcgcg cagttcggcg gcacgtggat cgaattcaga
     2881 taattgacga tcggggcgac gcccggttgg gtaaagtcgg agcagttggt gtagctgccg
     2941 ccgccgtatc cgttattggc gtaccaattg ttggtgcccg gcgccggatt ggggttctcg
     3001 acctcatcga caaaaaccgg cgggttcgaa ccgggaacca gaacgtggtt gggggtcgcg
     3061 ggatcgccat tctggtcagc gtagtacagc tcgtcggcat agccgatcat catgtggttg
     3121 gcgtaggttc cgcccatcgc cgcctgatgg tagttatcgt tcagcgtgta ttcgcgggcg
     3181 agcttggtca gatagggcgc gtcgccgttg gcgacgttgt aaaagcccat cgcaatatcg
     3241 ccttccttga ggggtttcgg cgtcggattg ccattgctgc cggaggaaac ggtctcttca
     3301 acccagggga acagatcagc ctgacatcca cttggattgc gctgggtggc cttggcgacg
     3361 tcgcagtcga gctgctgcca catctgatag aagcgatgca ccgggctgcc gccgtaagtc
     3421 ttgtacaggc tcgaggtttc gtcctcgccc tgcggcagcg tcaccagcgg gtaggggccg
     3481 ttcggcaggt tgctggagtc caggatgcgc gcgtccggcg aattccccgg tatgccggtc
     3541 gcccccgtcg tcagcaacgg cagatcctgc ttgcgcaggc cgtaatcgcc ccgtgcggcc
     3601 tctgccagcg tggcgaggca tcccggacca ttgagggcag cctgttcgac cgtggcatag
     3661 cagttctgcg gcgcgtagga tgttcccggc gcctgcagct tgttggtcgt ggtgttgtag
     3721 gcggtcttcg gcgtggggtc gatcgagaag aagttgttga ccgttgtcga gaactgcgcc
     3781 gccttggcgt aattcgggcc aggcgagccg tcctttctta tgattccctt ggacaggaga
     3841 ttgtcgacat gctcgccgtc gcgcggctcg taagtcgcga acacatggtc gaaagtccgg
     3901 ttttcgccga tgatgacgat gacatgtttg atggggctgg cggtcctgcg gtcgccggta
     3961 tgggttcctc ccattgcggt gttttcgacc gcgtaagcat cgcatcccat cagtgacaag
     4021 atcgctgcgg tgatgacggt gcgatccctt agacgtttcg ttagttgttt gagcataacg
     4081 cgcctcccag ttcaaagtta cattcgaaat gcgtcgatgt cgttcggcga gcagcgaatg
     4141 gcaatggaag gtaggtgcgg cacgtgtcat gtgtgcttcg aacgcatgtc agttctgtta
     4201 caggatgacg atgacgctga acggaacggg gttcggcgat cgtttccgga tcgagggcgt
     4261 cgccgcctcc aaggtgctcg ccggcatttc tctccggatc gcgggcgcct cttgaccgaa
     4321 tggctggccc ttcgaaaaca tcgacctgac ccgcagcact agccgatcca cggcaccagc
     4381 tttggcggag cctcggggac atcaagagcg gacatggatc gagggtgctg ctgtcgttcg
     4441 caggagttca gcgcatccct tcatggatga tgaagccgcg cgcgattgcc agtttccgca
     4501 gcgcgcggag aaggaatttt tgcggctgtt ggccaggata gcgccgcgat gtcagctgga
     4561 aatcccggag cgccccgtgg tcgctctcga acggggcgcg caatactgcg ggagattagc
     4621 cggccgccta agctcgggcg ggtattgttg gggtgggtat tctttgcttc cgtgatggcg
     4681 cgaaaccgtc agtcagccga tccgccgcgg gtgcgccgga acggcgtctt agaaaaaacc
     4741 ggcgcttata tccaggccga aagtagccag acagaacgat gtgcccagaa caagtgcaca
     4801 aagcagagcg atggacatga gaggattaag agtctgagtt tcgggaatgg cgcgtaccga
     4861 gacatccgaa gcagtattca tggcaacctc cactagaaaa atactcaact gatcgttttg
     4921 attgtttgca aaaactgttg tgtcgcaggc acaacttgaa tttcgattgt ggcgaatgaa
     4981 aacttcgatt gtggccaatg agaggtgaca gcgtgaccag ctggctttgc gcaactgcgc
     5041 taccctcggt tgaaatgcat gcgacagcga agggcgtgaa accgggcgtt gctgcggtcc
     5101 gattttgtca acggatcctt tctagcgcag tccctcatag accatgaatc cgcgcgcgac
     5161 tgccagtttt cgcaacgcgc ggggaagcag cttctgcggt tggtggccga gatagcgcca
     5221 cgatgtcagt tggaaatccc ggagcacgct gtggttgttt ccgaacggcg cgagcaatcc
     5281 ggtcagggtt agcggcgtgt gcgcgcgggt gttgaacggc agcagcagca attccagatg
     5341 cgcgcgcgag ccatcttgcg acgtcgcggt gatgccggcg acagcggcca gcatttcttc
     5401 cgataccacg gcgatgatat cctcgatctc gcggcggcct tccggctcga acagcgcgga
     5461 aaagctccgg tccttcagat cgcagccgag cagggcgcag actctggttc cggcgacgcg
     5521 aaacggataa ccggcggcgg cgtcatacga cagcacgaag atatcgccga gcagttcgcg
     5581 caccgcgccg ggctcgatct cgctgcgctc cggtgcgcgc gcgttgccgc gctgctcgtc
     5641 ccaatatgcg aaaaactcgc ggcttgacgg atgtttcatg aggctacctc gtccggcgcc
     5701 cggctcggtg ggacacctct gtcccgcttt cgcccacgcg cattcccctg attgttttgc
     5761 aagacaggct ttgcagcgtc catgccgcgg aggcgctatc gggcgattgg cggcgccttt
     5821 gcgatgttaa ggttaaatta actaaggggt tggggatcgg tgcaggcgtg ctagtgtccg
     5881 cgcactccat tgctcgccgg ttttctgcag ggtccggcga ggtcgttaca gaaggcggcg
     5941 tcaagcaagt tggtcgtcgt gctgggaggg tggatgtcag tcacccctta cccttgcgcg
     6001 aaaagatcag caccgacgag ggagggcttt ctcagagccc tccctttttt gttgggtcgc
     6061 gcaaccccgc cgcggggaca ttcgagaagc cgtcggctcg ctttcgccgc cgatatcagc
     6121 gcgacgtaat ccagcggtca catccccgga catatgatgt gcgggtcgga tgcgtggagt
     6181 gagttttatg acgtcgaatg aaaccatgtc ggcggttcgg gccctcctgg aatcctatcg
     6241 cgacgcgttc gagcgactgg atgccgccgc cattgccgac cattttgtct atcccagcca
     6301 tatcactggc gatgccgatg aaatcgttct gctgcagctt tcgaaccggc aggattgcct
     6361 ggctgctgtc gagaaggttc ttgcgatgca tcgtgaactc gaagcaccaa ctggctctat
     6421 ttgtgatctt tcgatcatcg aactctcgcc gcggctgacg cgggcttcct ttcggatgga
     6481 agtgcatcgt cgcacgggtg ggatgcttta cgatttcgag gccgcatata cacttgcgga
     6541 aacgcgcgcc ggatggcgga tcgcggccat cgcgcacaat cagatccccc ggctcctgaa
     6601 atgcttggca cagcgtcaag ccggtgcgtg agccgacgtc gtccgccata tttcagaacg
     6661 agcatgcggt cgatcgtcga cggccggcga gaaaggccgt gagcgccaac gcctggagca
     6721 gtgcggcggc agcaaacaga tacggagcgg ctgcacccgc caaaacgccc gcctgcgggg
     6781 caaattccct gattaatccg aagctggccg gcgcgaacgc gtaggtccct tgcgaaatcg
     6841 cgacgttcag ggaaacgacc cttaaaacat cctgcttggc gaattcggcc tgagcgatca
     6901 gcggtggcag cgaggtggcg ttgccgatgc cgaccccgaa caggatgacg ccaagaacaa
     6961 gcaagggaat atgggtgcca cccgcgatca ggaatgcgat ggagcccgtg atctggacgg
     7021 taaagctgcc gcaggccacc agacgccggt cggcgccgac aggcatcaac cagccgacga
     7081 gcgtgcgccc tgcgatcgcg gccatcgtcg cagcgctgat cgcgatgcct gccaattgag
     7141 cgccgacggc ggggacgagc aatgaaaaca gatgggcgag caggccgatc tgcgcgaaga
     7201 ggccaagcgc cgtgccggcc gccaaggtga caaacctgcg gtccttccag aggctcgcgc
     7261 cggaaagcgg tctcgccgcc gcaaaactgc cgaaaactgc cgcgaccccg ggctcatcgc
     7321 catcgggcgc caatcccatc tgttgcggcg ttctgcagaa atagacgtcc gcgaggatcc
     7381 agacacagat cgtcacgatc acgccgacga ccgcggttgc ggccggaaaa ccccacatcc
     7441 cgatggcagc gacccagagc ggcgagaaca ccacgccgcc gatgctggcc ccgttgtagg
     7501 ccatcgcaag cgccgcgggc cgcgtccgca cgaaccacgg cgccaggatc gcgttgaccg
     7561 cagcggcgcc catcgcaacc caacctgcgc cgctgaaaag cgccgccgca aacagctgcc
     7621 agggctcggc cgcgacggcc caacccgaaa tgccgagagc gagcgagagc acgccggcct
     7681 tcgtgaccgc cgggagcccg tagcgaagat aaagttttgg caagcgcgcg acgacgagcg
     7741 cgccaaacag gaagtgaacg gtgacggccg ccgacaccag cggcagcgac cagcctctga
     7801 gatcacgaac cgcctgaaga tagatcggcg gaccgtagaa cccgagaccc cagccgaata
     7861 cggccagcac aaaggccgcc cccacgatgc gccatccata gaacttttcg cggtacgtta
     7921 atgccgtcat cgtctgctcg cctctttacc gcttgtgtcg ctggcgattg cgttcgatcg
     7981 ccggggcgga agcgcgccgc ccgaataccg gtcgggcgtg caaggatgct acggcgagga
     8041 ccgaagcatc gccggcctgc gacgcgatag gctccagctc cgtgggagga gtaggcacga
     8101 tggcgagtac ggcggccttt gccgaagtcg cggcgatggc gggcgatccg gcgcgcgccg
     8161 cgatgcttca cgcgctgatg gacggccgtg ccctgacggc ttcggaactc gcgcgcgtag
     8221 cagggatcgc gccgcagacc gcgagcgggc atcttgcgcg tatgacgcag accgggcttc
     8281 ttgcggtcgc gaggcaaggg cggcatcgct atcaccggtt ggcgacgtca ggtgtagccc
     8341 gcatgatcga ggggatcatg cgggtagcgt ccgaactgca gccgccgcgc aagaaccttt
     8401 cggttggccc gcgcgatgtg gcgctgcggc aggcgcggac ctgctacgac catcttgccg
     8461 gccgtcttgg cgtttccctg tccgacgcac tgacctcggc ggggtatgcc gagctctcgg
     8521 ccgagtcggg gattatcacc gagcgcggtc tggcatttct cgagcgggtc ggaatcgacg
     8581 tttcggccat gcgggcgcgg ggtggaatga agtcgggccg cctgctgtgc cgtccatgtc
     8641 tcgactggag cgagcggcgc agccatctgg cgggtgtcgt cggcgcagcc ctctgcgccc
     8701 atagttttgc gcaaggcttc gtctgcagga tcgacggcac gcgcgcactg cggatcaccc
     8761 cgaaggggtt acgggtttat cgagagacgt tcggagttcg gtttgagtag cgccttcggc
     8821 cgtagcagcc gaaggcgctt tctgagatca tgatccgatt cgatttaggc catgaactca
     8881 tcaatgcgcc gatcggactg actgcccgag tccgctttgt ccccgatagc cgacgaatcg
     8941 ttgcatcgca gcgaaacgac gtgacgtgcc aaattcggac tcaacaccgc cgcctctatc
     9001 gatcacctca tcggcaaggc ataccccacg ccgtggcctc tgatgttcag cgcgatgaac
     9061 tctcgccgct tcattagatc acctcatgcc ctactcgccg acctcgctac tgatgacatc
     9121 gccgaacaat tcccaacgct cgcccttgaa tttcctgagc tgaaattgct tgatcggagc
     9181 gaagtcagtt gcgctggtgt tgatcgtgac gccgggcaag agcccttcca gctgcagact
     9241 cttgatgctc gcggcctgtt tcatgatatt cgcgcgggta agatcgtcgc cgcacttttt
     9301 gagaacataa accatcgttt gagcaacatt gtagccagtc attagattga ggtcctcacg
     9361 attaccctcc gggaaatatt tcgtaaggaa ggtgtcaaag ttcttgatac ctgcgtcatc
     9421 attccactgc gggtctgtag gatctttctg ataagttgcg gatattatgt tttggctgtt
     9481 ttctaatcca gctggcttca tcacggttcc cgttgatgca cctggaccgt tgagaatgaa
     9541 cagcggtttc caaccaagct ccgctacttt cttgattgac tgcgccgcga atttcgctgt
     9601 cgtgatgcta atgaaaatat ctgcaccctc cgacttgagc gccactacgt gcgagtcaat
     9661 ggtaggcgtg gtggtttcgt agctttcctc tccgacaatc atcgaagccg ccttagtgcc
     9721 cagaccgtcc gtgatgccct tgatataatc ttttccgtag tcgtcattct gataaaggat
     9781 accgatcttg ccagtcggcc tttccttcaa aatataccta gcataaatgc gaccttcgct
     9841 ctggtaattg ggcggccagc ccatcgtcca aggaaactgc ttcgagtcgt tccactttgt
     9901 cgcaccggtt tgaacgaaca gctgaggcac cttcttctcg ttcatatact tttgaattgc
     9961 tgagttagac ggcgtaccga gagcattgaa aatgaacagc acttcgtcgc tttcaaccag
    10021 tttgcgggct tgttcaaccg ccttcggcgg gctgtaggcg tcatcatatg agataaattt
    10081 gattttgcgg ccattgatgc cgccctctgc gttgatcttc ttgaagtacg cagcctctgt
    10141 cttaccgatg acgccgtaag cggaagctgg cccgctataa ggcatgatat tgccgatttt
    10201 gatttcagta tctgtcgcac caatgtcgta tttcttctgg gcaattgcgc cggtgctggt
    10261 tgctgccaac acaataatgg ttgccgacag cacctgtagt cgatcacgaa aagccggcat
    10321 gagagacctc cttcggtcgg ctgcatcagt tcgttttgat gatcttgtac gtccctttct
    10381 tgtggccaac gacttgaccg atgccatcgt aatcgctgtt gatggcttgg ttcacacgac
    10441 aaactctttg ccgccaccaa acgc
//
LOCUS       DASWGM010000926         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1476244,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000926 DASWGM010000000
VERSION     DASWGM010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476244"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VGA15_25105"
     CDS             complement(<1..301)
                     /locus_tag="VGA15_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX9591003.1"
                     /translation="MIIAIDGPAASGKGTLGKRLAKHYGYRHLDTGVIYRAVAKALLD
                     AGADLTNEMLAVSAALELDPEKFGNPALKTQEVGDAASVVSAIPKVREVLVNFQRQ"
     gene            complement(308..715)
                     /locus_tag="VGA15_25110"
     CDS             complement(308..715)
                     /locus_tag="VGA15_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591004.1"
                     /translation="MRPNTLAEAVERIQAGLAQDIALAEFVDTFDLAKSESDRYASIE
                     REPKLTGDDRLDALVGAIAEYLAKQRRLGRVPQWASNPARRLTTPWFTTASPSDAMRE
                     YLTFASPAEFASRNIFTEERPLRRARGPQPAKI"
     gene            complement(693..1298)
                     /locus_tag="VGA15_25115"
     CDS             complement(693..1298)
                     /locus_tag="VGA15_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591005.1"
                     /translation="MTAAFDRDALLDAFDRIGRAAALAGTKLQIAVYGGSALMLASNF
                     RFATEDVDVSELEHPLPGWLATVVDEIAKENGWLDDWFNDGVAFHLSPLADRALDHLE
                     FGTFPRDGTSPGLAVSVPSAEYLLALKLKAARVTDPLRGETERLDILNLMQVVGISTV
                     EDAIVLLGRYFPVSAASSEKQRFLLKNMNRKGGNDAPKYPG"
     gene            complement(1306..>2341)
                     /gene="aroA"
                     /locus_tag="VGA15_25120"
     CDS             complement(1306..>2341)
                     /gene="aroA"
                     /locus_tag="VGA15_25120"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX9591006.1"
                     /translation="TGCRLVMGAVAGCPITAIFDGDASLRGRPMRRVLDPLELMGAKV
                     GAAKEGGRLPLTLNGARDPLPVLYRTPVASAQIKSAVLLAGLSAPGVTTVIETEASRD
                     HTELMLKHFGAQIVSTREGSHGRKIALTGQPELHGADVVVPADPSSAAFPIVAALIVE
                     GSDIVLSDIMTNPLRTGLFTTLREMGASIEESHARGDAGEPMAQFRVRASRLRGVEVP
                     PERAPSMIDEYLVLAVAAAFAEGTTIMRGLQELRVKESDRLEATAAMLRVNGVKAEIS
                     GDDMIVEGKGHVSGDGLVATHMDHRIAMSALVMGLASDKPVKVDDTAFIATSFPDFIP
                     MMRGLGAEFS"
BASE COUNT          453 a          757 c          701 g          430 t
ORIGIN      
        1 attggcgttg aaaattgacc aacacctccc gcactttcgg aatcgccgaa accaccgagg
       61 cggcgtcgcc gacctcctgg gttttcaggg ccggatttcc gaacttttcg ggatcgagtt
      121 cgagtgcggc ggagacagcc aacatctcgt tggtcagatc ggcgccggcg tccagcagcg
      181 ccttggcaac cgcgcgatag atcacgccgg tatcgaggtg gcgatagccg taatgcttgg
      241 cgagccgctt gccgagcgtg ccttttcccg aagccgcggg tccgtcgatg gcgatgatca
      301 tatgggccta tatcttggct ggttgagggc cacgggcccg tcgcagcggc cgttcctcag
      361 tgaagatgtt gcgcgatgcg aattcggccg gactggcaaa ggtcaagtat tcgcgcatgg
      421 cgtccgacgg cgacgccgtc gtaaaccatg gagtggtcaa ccggcgcgcc ggattcgatg
      481 cccattgtgg aacccggccg agccgtcgct gctttgccag atattcggcg atcgctccga
      541 caagcgcatc aagccgatcg tcgccagtca atttcggttc gcgctcgatc gacgcatagc
      601 ggtcactttc ggatttcgcc aaatcaaacg tgtcaacgaa ttcagcgaga gcaatgtcct
      661 gcgccaaacc ggcctgaatc ctttcgaccg cttcagccag ggtatttggg cgcatcgttc
      721 cctcccttac ggttcatgtt cttcaataga aaccgctgct tctcggaaga agcagcgctc
      781 accgggaagt atcggccaag caagactatc gcgtcctcta ccgtcgagat gcccacaacc
      841 tgcatcaaat tgagaatatc gagccgctcc gtttcgccgc gaagcggatc cgtgacccgc
      901 gccgctttta gcttcaacgc aagtaaatat tctgccgacg gaaccgaaac cgcaagtcct
      961 ggggacgtac catctcgcgg aaacgtccca aactccagat gatcgagggc gcgatccgca
     1021 agagggctga ggtggaaggc gaccccgtcg ttgaaccagt cgtccagcca accattttcc
     1081 ttggcgattt cgtcaacgac cgtcgccagc caacccggta gcggatgctc aagctcggat
     1141 acgtcgacat cttccgtggc aaaccggaaa ttgctcgcaa gcatgagcgc ggagcccccg
     1201 taaaccgcga tctgcagctt ggttccggcc agtgccgccg cgcgaccgat ccggtcgaat
     1261 gcgtcaagca gggcatcgcg gtcgaaggct gcagtcatcg cgccctcatg aaaattctgc
     1321 cccaaggccg cgcatcatcg ggatgaaatc cggaaagctg gtcgcgataa aggcggtatc
     1381 gtccaccttc accggcttgt cggaagcaag ccccatcacc agcgccgaca tcgcgatccg
     1441 gtgatccata tgggtggcga caagaccatc gcccgaaaca tgacccttgc cttcgacgat
     1501 catgtcgtcg ccggagatct cggccttcac gccgttgacg cgcagcatgg cggcggtcgc
     1561 ctccagccgg tcggattcct tgacccgcaa ttcctgcaag ccgcgcataa tggtcgtgcc
     1621 ttcggcgaag gcggccgcca ccgcgagcac cagatattcg tcgatcatcg agggcgcgcg
     1681 ttccggcggc acctcgacgc cgcgcagtct tgaggcacgc acccggaact gcgccatcgg
     1741 ttcgccggca tcgccgcgcg cgtggctttc ttcgatcgac gcgcccatct cgcgcagcgt
     1801 cgtgaacagc ccggtacgca gcggattggt catgatgtct gacaggacga tgtcggaccc
     1861 ttcgacgatc aaggccgcca cgatcggaaa cgccgccgac gacggatcgg ccggcaccac
     1921 gacgtcggcg ccgtgcagtt cgggctggcc ggtgagcgca attttgcggc catggctgcc
     1981 ttcacgcgtc gagacgatct gggcgccaaa atgcttcagc atcaattcgg tgtggtcgcg
     2041 gctggcttcg gtttcgatca cggtggtgac gccgggcgcg gagagccccg ccagcagtac
     2101 agcggatttg atctgcgccg aagccaccgg cgtgcggtag aggacaggca acgggtcgcg
     2161 cgcgccgttg agcgtcagcg gcagacgacc gccttcctta gctgcgccga ccttggcgcc
     2221 catcaattcc agggggtcga gcacccgccg catcggcctg ccgcgcagcg aggcgtcgcc
     2281 atcaaagatc gcggttatcg gacaaccggc caccgctccc atcaccagcc ggcagccggt
     2341 a
//
LOCUS       DASWGM010000927         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_953131,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000927 DASWGM010000000
VERSION     DASWGM010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953131"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VGA15_25125"
     CDS             <1..375
                     /locus_tag="VGA15_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9591007.1"
                     /translation="ICFFPILLTTARGLSEVEPDLLDLVKSLRGSRWTLFRKIQLPGA
                     LPYVFSGMKVGAILAVAGAIVGEFIASERGLGYLMIQVQSSLDTPAMVMAVVLLTLLG
                     VALYGLVLGLERLFVVKDVRLR"
     gene            395..1432
                     /locus_tag="VGA15_25130"
     CDS             395..1432
                     /locus_tag="VGA15_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9591008.1"
                     /translation="MLLTRENLPETIADIAALDDLLCRPSQALIDDLQKVDGDIMILG
                     VAGKMGPTLAGLAKAALPHRRIIGVARFSDASAKAWLEARGIETLNCDLLDEAAVKAL
                     PKVKNIVFMAGRKFGADGDLSLTWAMNAHVPALVAQAFAQSRIVAFSTGCVYPFVPVN
                     GNGADENLAPDPPGEYAQSCVGRERMFEYFSRQFATPGRLFRLNYAIDMRYGVLHDIA
                     TQILGGNPIDVSIGHVNFIWQGDASAQALRCLAHCDTPTSPINVSGHEILSVRDLAAK
                     LGARLGRDPIIVGTEQPTAWLTDTSQAAKLFGRPIVDTERLIAWTADWVARAMPSLGK
                     PTKYEVRDGRY"
     gene            1419..2252
                     /locus_tag="VGA15_25135"
     CDS             1419..2252
                     /locus_tag="VGA15_25135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9591009.1"
                     /translation="MAGTDHMAIIEVGPDDALAGLALSTEAHWNQNEADWRFFLSQGT
                     VFGVRDHDGHLVASAALLPYTSGNAWISMVLVTARFRRRGFATRLMDACLDAAAKQGF
                     TTWLDATPAGATVYAPLGFTPTSELRRLRLENLAVPKATMLQSLSSYNLDEFIARDIG
                     TMGFDRRALLAELGGRPGSRLVSSGGAAALVRDGRKARHIGPLFADGPAPALALVHEI
                     IRSQTGPVLIDAVSTQDEFINGLTSYGWIVERPFQRMRFGAATARPAELPFAVAGPEY
                     G"
     gene            2264..3319
                     /locus_tag="VGA15_25140"
     CDS             2264..3319
                     /locus_tag="VGA15_25140"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9591010.1"
                     /translation="MHQSAMKSEVRRLIADGTVIPAHPLALDANRKLDTVHQRALTRY
                     YIDAGAGGLAVGVHTTQFAIRDVGLYRPVLELAAETAANWTKRPLALVAGLAGPTQQA
                     IAEAETARGIGYHAGLLSLAAMKSASEDEIIAHCEAVAREIPLVGFYLQPAVGGVILS
                     ANFWRRFAEIDNVIAIKIAPFNRYRTLDVLRGVCAAGALDRIALYTGNDDHILLDLAL
                     PFDLRDRGVTVRTYFRGGLLGHWSVWTENAIKQFEMCRAARNKDTVPSDLLALDARVT
                     DCNSAFFDVANNFHGCIAGCHEILRRQGLLEGIWCLDPAEGLSPGQMQEIDRVCNEHA
                     DLSDDDFVAARLPKWLA"
     gene            3307..4092
                     /locus_tag="VGA15_25145"
     CDS             3307..4092
                     /locus_tag="VGA15_25145"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591011.1"
                     /translation="MAGMTEEPFIRLRSVRKVYRSHGAEFLAVSDITMDVREGELISL
                     VGPSGCGKTTVLKILAGLHDADGGTIQIGNARTPFDPARDIGMVFQQALLLKWRTVLD
                     NVLLPAEIVGLPIKAARARARDLLNLVGLAGYEEKYPQQLSGGMQQRTAIARAFIHDP
                     KLILMDEPFGALDALTREQMNLEMLRIWRESGKTIIFVTHSIQEAVFLASHCAVLTAS
                     PARMAEYFPIDLPFPRALPIKTTDEFGAYARRIYASLGLGSSA"
     gene            complement(4105..>4537)
                     /locus_tag="VGA15_25150"
     CDS             complement(4105..>4537)
                     /locus_tag="VGA15_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX9591012.1"
                     /translation="VDVGGWDTHVNQGAATGYLADRLGELGRALAGFSEEIGPAGWRD
                     TVVVVISEFGRTFRENGDRGTDHGHGSVYWVLGGGINGGRILGEQVKIEQASLFQNRD
                     YPVLTDYRAMFAGLFQRMFGLETASVQRIFAGVHPAELGLV"
BASE COUNT          814 a         1482 c         1398 g          843 t
ORIGIN      
        1 atctgcttct tcccgatcct gctgaccacc gcgcgcggac tgagcgaggt cgagcctgat
       61 ctgctggatc tcgtgaaatc gctgcgcggc tcgcgctgga ccttgtttcg caagatccag
      121 ttgccgggcg cgctgcctta cgtgttctcc ggcatgaagg tcggcgccat tctggcggtc
      181 gcaggcgcca tcgtcggcga gttcatcgcg tccgaacgcg ggcttggtta tctgatgatt
      241 caggtgcagt cctcgctcga cacgcccgcg atggtcatgg cggtcgtgct tctgaccttg
      301 ctgggggtcg cactttacgg cctggtgctt ggcctcgaac ggctgttcgt ggtcaaggac
      361 gtgcggctgc gataaaatcg gtcagggaag acacatgctg ctgacgcgcg aaaatttgcc
      421 ggagacgatt gccgatatcg cagcgctcga cgatctcttg tgccggccga gccaggcgct
      481 gatcgacgat ctccaaaagg tcgatggcga tatcatgatc ctcggcgtcg ccggaaagat
      541 ggggccgacg ctcgccggcc tcgccaaggc cgccctgccc catcgacgca tcatcggcgt
      601 tgcgcggttc tccgacgcca gcgccaaagc gtggctagag gcgcgcggca ttgaaacgct
      661 gaattgcgat ctgctcgatg aagccgccgt caaggcgctg ccaaaagtca aaaacatcgt
      721 cttcatggcc ggacgcaagt ttggcgccga cggcgacctg tcgctgacct gggcgatgaa
      781 cgcccatgtc cccgcccttg tcgcgcaggc ttttgcgcaa tcgcggatcg tggcgttctc
      841 gaccggctgc gtctatccgt tcgtcccggt gaacggcaac ggcgccgatg aaaatcttgc
      901 acccgatcca cccggcgaat acgcgcaatc ctgcgtcggg cgcgagcgca tgttcgaata
      961 tttctcgcgc caatttgcaa cgcccggccg gttgttccgc ctgaactacg cgatcgacat
     1021 gcgatacggc gtgctgcacg acatcgcgac ccagatcctc gggggaaacc cgatcgacgt
     1081 cagcatcggg catgtcaatt tcatctggca gggcgatgcc tcggcgcagg cgctgcgctg
     1141 cctcgcgcat tgcgatacgc cgacctcgcc gatcaatgtc agcggccatg aaatcctcag
     1201 cgtgcgcgat ctcgcggcaa aactcggcgc gcgtctcggc cgcgatccca tcatcgtcgg
     1261 taccgaacaa ccgacagcct ggctcaccga tacctcgcaa gccgcaaaac tgttcggccg
     1321 gccgatcgtc gataccgagc ggctgatcgc ctggaccgcg gactgggtcg cgcgcgcgat
     1381 gccgagcctc ggcaagccca ccaaatacga ggtgcgcgat ggccggtact gaccacatgg
     1441 ccattatcga agtcggtccg gacgacgcgc tcgctgggct tgcgctgtcc acagaggcgc
     1501 actggaatca gaacgaggcc gactggcggt tttttctgag ccaaggaacc gtgttcggcg
     1561 tgcgcgatca tgacggccac ctcgtcgcca gcgcggcgct gctgccctac acgtccggca
     1621 atgcctggat cagcatggtg ctggtgaccg cgcgtttccg ccgccgcggg tttgcaacca
     1681 gactgatgga tgcctgtctc gatgccgcgg cgaaacaagg ttttacaacc tggctggacg
     1741 cgacgccggc gggcgccacg gtttatgcac cgctgggctt tacgccgaca tcagagctta
     1801 ggcgattgcg gcttgagaat cttgcggtgc cgaaagccac aatgcttcaa tcgctatcga
     1861 gctataacct tgatgaattc atagcgcgcg atatcggcac aatgggcttc gatcgccgcg
     1921 ctctgctggc ggaactcggc ggaaggcccg gctcgcggct ggtgtcgagc ggcggtgcgg
     1981 ccgcgctcgt ccgcgatggc cgcaaggccc ggcacatcgg tcccctgttt gccgacggcc
     2041 cggcccccgc gcttgcactg gtccatgaga ttatccgatc ccaaaccgga cccgtgttga
     2101 tcgacgccgt cagcacccaa gacgaattta tcaacgggct aaccagctac ggctggatcg
     2161 tcgagcggcc gtttcagcgg atgcggttcg gcgccgccac ggctcgacca gcggaactgc
     2221 cgtttgccgt cgccggcccg gaatatggat aaggaattgc acgatgcacc aaagcgcgat
     2281 gaaatccgag gtgcggcgcc tgattgccga tggcacggtg attcccgcgc acccgctggc
     2341 gctcgacgcc aaccgcaagc tcgacaccgt ccaccagcgc gcgctgacgc gttattacat
     2401 cgacgccggt gccggcggcc tcgcggtcgg cgtccacacc acgcagttcg ccatccgcga
     2461 cgtcggcctc taccggccgg tgctggaatt ggctgcggag accgcggcta actggaccaa
     2521 acggccgctg gcgctggtcg caggtctcgc tggcccgaca caacaggcca tcgccgaggc
     2581 ggagaccgcg cgcggcatcg gctatcatgc gggcctgctg agcctcgcgg cgatgaaatc
     2641 ggcttccgaa gacgagatca tcgctcattg cgaggcggtg gcgcgcgaga ttccgctggt
     2701 cggcttctat ctgcagccgg cggtcggcgg cgtcatcctc agcgcaaact tctggcggcg
     2761 gtttgcggaa atcgacaatg tcatcgccat caagatcgcg ccgttcaacc gctaccgcac
     2821 gctcgatgtc ctgcgcggcg tttgcgctgc cggcgcgctc gatcggatcg cgctctatac
     2881 cggcaatgac gaccacatcc tgctcgatct tgcgctgccg ttcgatctgc gcgatcgggg
     2941 cgtcaccgtc agaacctatt tccgcggtgg cctgctcggc cattggtcgg tgtggaccga
     3001 aaacgccatc aagcaattcg agatgtgccg cgccgcgcgc aacaaggaca cggtgccatc
     3061 ggatctcttg gcgctcgacg cccgcgttac cgactgcaac agcgcgttct tcgacgtcgc
     3121 caacaatttt cacggctgca tcgccggctg tcatgagatt ctgcggcggc agggattgct
     3181 cgagggtatc tggtgtctcg atcccgccga gggcctcagc cccggccaga tgcaggaaat
     3241 cgaccgcgtc tgcaacgagc atgctgatct tagcgacgac gatttcgtcg ccgcccgctt
     3301 accaaaatgg ctggcatgac ggaagagccc ttcattcgtc tgcgcagcgt ccgcaaggtc
     3361 taccgctcgc atggcgcgga atttctcgcg gtctccgaca tcaccatgga tgtacgggag
     3421 ggcgagctga tctcgctggt cggcccaagc ggttgcggca agaccaccgt cttgaagata
     3481 ctcgccgggc tgcacgacgc cgacggcggc accattcaaa tcggcaacgc gcgaacgccg
     3541 ttcgaccccg cgcgcgacat cggcatggtg ttccagcagg cgctgctgtt gaaatggcga
     3601 accgtgctcg acaacgtgtt gttgccggcg gagatcgtcg gcttgccgat caaggccgct
     3661 cgcgcccggg cgcgcgatct gctcaatctg gtcgggcttg ccggctatga ggaaaaatat
     3721 ccgcagcaat tatccggcgg gatgcagcag cgcacggcga tcgcgcgcgc cttcatccac
     3781 gatccaaaac tgatcctgat ggatgagccg tttggcgcgc tcgatgcgct gacccgcgaa
     3841 cagatgaacc tcgaaatgct gcggatctgg cgcgaaagcg gcaagaccat catctttgtc
     3901 acgcacagca tccaggaggc ggtgttcctc gcctcgcact gcgccgtgct gaccgcgagc
     3961 ccggcgcgaa tggccgaata tttcccgatc gatctgccgt tcccgcgcgc gctgccgatc
     4021 aagaccaccg atgaattcgg cgcctatgca cggcggattt atgcgagcct ggggcttggt
     4081 tccagcgcgt agcccgctgc ctgactagac caatcccagc tccgccggat ggacgcccgc
     4141 aaaaatccgc tgaacgctcg cggtttccag cccgaacatg cgctggaaca gcccggcaaa
     4201 catggcacga tagtcggtca gaaccggata gtcgcggttt tgaaaaaggc ttgcctgctc
     4261 gattttgact tgttcgccga ggatacggcc gccattgatg ccgccgccca gtacccagta
     4321 gacgctgcca tggccgtgat cggtgccgcg gtcgccgttc tcccgaaagg tgcggccaaa
     4381 ttcggagatc acgaccacca ccgtatcgcg ccatcccgca ggtccaatct cttcggaaaa
     4441 acctgcaagc gcgcggccga gttcgccgag gcggtcggcg agatatcctg tcgccgcacc
     4501 ctgattgaca tgggtgtccc agccgccgac atcgaca
//
LOCUS       DASWGM010000928         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_653754,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000928 DASWGM010000000
VERSION     DASWGM010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653754"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VGA15_25155"
     CDS             complement(<1..1302)
                     /locus_tag="VGA15_25155"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9591013.1"
                     /translation="MSDAVKVGFVPFSTAPRGILVIFCDDALKFGPATGKALGDATGL
                     VKRAAATNQFKGKSGSTLDILAPEGLKASRLIVVGAGKVSAIKANDFLKLGGAAAGKL
                     GAGTGAVMIIAELPDGAMKPEQAVAVAAGIRLRAYKFDRYKTKKKDGEDAALHADVSL
                     AVGDVAAARKAFAPDGHIVDGVIMARELVNEPPNVLFPVEFARRAGQLRKLGVIVEVL
                     DVKAMTKLGMGALLGVAQGSTQPGRVVIMRWNGGKRGDQPVAFIGKGVCFDTGGISIK
                     GAASMEDMKGDMGGAACVVGLMHALAARKAKVNAVGAIGLVENMPDGNAQRPGDIVTS
                     MSGQTIEIINTDAEGRLVLADVLWYVAKKFKPKFMVDLATLTGAIMVALGTEYAGLFS
                     NNDELAERLTKVGLETGERVWRMPLGPEYDKQIDSQFADMKN"
     gene            complement(1509..>1721)
                     /locus_tag="VGA15_25160"
     CDS             complement(1509..>1721)
                     /locus_tag="VGA15_25160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591014.1"
                     /translation="RLTRTSANDASVVRKIYLSIDPIVTARSPPPELERQLATNPEIG
                     IPFWSRRHWPSRLTPHTIPLIRQQNG"
BASE COUNT          311 a          553 c          514 g          343 t
ORIGIN      
        1 gtttttcata tcggcaaact gcgaatcgat ctgcttgtcg tattccgggc cgagcggcat
       61 gcgccagacc cgttcccccg tttccagtcc gaccttggtc aaccgttcgg ccaattcgtc
      121 gttgttggag aacaacccgg catattcggt gccgagcgcc accatgatcg cgccggtcaa
      181 ggtcgccaga tcgaccatga atttcggctt gaatttcttg gccacgtacc agagcacgtc
      241 ggccagcacc aatcggccct cggcgtcggt gttgatgatc tcgatggtct ggccggacat
      301 cgacgtcacg atatcgccgg ggcgctgcgc attgccgtcg ggcatgtttt cgacgaggcc
      361 gatggcgccg accgcattga ccttcgcctt ccgcgccgcc agtgcatgca tcagcccaac
      421 cacgcaggcc gcgcccccca tgtcgccctt catgtcctcc atgctggcgg cgcccttgat
      481 ggaaataccg ccggtatcga aacagacgcc cttgccgata aaggcaaccg gctgatcgcc
      541 ccgcttgcca ccattccaac gcatgatcac aacccgtccc ggttgcgtcg agccctgcgc
      601 gacaccgagc agcgcgccca tgccgagttt cgtcatggcc ttgacgtcga ggacctcgac
      661 gatgacgccg agcttgcgaa gctggccggc gcgacgggcg aattccacgg gaaacagcac
      721 gttcggcggc tcattgacga gttcgcgcgc catgatgacg ccatcgacga tgtgtccgtc
      781 cggagcgaag gccttgcgtg ccgcggctac gtcgccgacg gcgagcgaaa cgtctgcatg
      841 caatgcggca tcctcgccgt ctttcttttt ggtcttgtag cggtcgaatt tataggcgcg
      901 cagccgtatt ccggccgcga ccgcgaccgc ctgttccggc ttcatcgccc catcgggcaa
      961 ttccgcgatg atcatcacgg cgccagttcc ggcgcccagt ttgccggctg ccgcaccgcc
     1021 aagcttgagg aaatcattgg ccttgatggc cgaaaccttg ccggcgccga ccacgatcag
     1081 gcgcgaggcc ttgagaccct ccggcgccag gatatcgagc gtggacccgc tctttccctt
     1141 gaactgattc gtggccgccg cgcgcttgac gaggccggtc gcatcaccga gcgccttgcc
     1201 ggtcgccggg ccgaatttca acgcgtcgtc gcaaaatatc accaaaatgc cacgcggggc
     1261 tgtggaaaat ggaacgaagc cgaccttgac ggcgtcggac ataggcaaat cctccaaaaa
     1321 atcagggctg tcgctgtgcc tggaacccgc cgggcggtgt ttcgaatgct cgcttcggat
     1381 gcaataccca tccgtcagaa gcatggtcgg acatactatt gcccgtcagc cgccgcgctg
     1441 ccaagcgtcc ccgtggcctg gcggccacat agcgcgaata attaaccaca tattgggggt
     1501 gccttggctc agccattttg ttgacggatc aaagggatgg tatgaggagt aagccggctg
     1561 ggccaatggc ggcgcgacca aaaggggatc ccgatttcgg gattggtcgc gagctgccgt
     1621 tccagttcgg ggggcgggga tcgtgcggta acgatggggt caatcgacag gtatatcttc
     1681 cgcacgacgc ttgcgtcgtt tgcgctggtt ctggtcagcc t
//
LOCUS       DASWGM010000929         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1912947,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000929 DASWGM010000000
VERSION     DASWGM010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912947"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..79)
                     /locus_tag="VGA15_25165"
     CDS             complement(<1..79)
                     /locus_tag="VGA15_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX9591015.1"
                     /translation="MSKPEFVYITYIETTAEKLWHALTDG"
     gene            complement(72..386)
                     /locus_tag="VGA15_25170"
     CDS             complement(72..386)
                     /locus_tag="VGA15_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX9591016.1"
                     /translation="MDEVFKALADASRRSLLDRLHAKNGQTLNELCDGLAMTRQAVTK
                     HLAILEAANLVTTFRHGREKLHYLNPVPIHQIGERWIRKFERGKLAALGELKRQLEKR
                     DE"
     gene            complement(495..1724)
                     /locus_tag="VGA15_25175"
     CDS             complement(495..1724)
                     /locus_tag="VGA15_25175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX9591017.1"
                     /translation="MSPHHIDFASRRRLLQFLAASPLFARGALAEGPRPSDPVDWAPR
                     DPDKLIADPTQALDVFDFEPVMKKNVPPAHFGYMATGADDEVTLRANREGFRKFELRP
                     RRLVDVSKIDISAEILGTKYDSPIVIAPAGSNRAFHADGEIAVAKAAKAGNHLQILST
                     VATTSIEDAIAARGAPVWFQLYTTQRWEVAEGLVKRAEAAGAPAIAVTLDVRSFAKWE
                     TFVRLRRTDARDCGSCHGLTDYLSRKPNFTGIDLGGVSSTVVTNLTWDLIKRLRDTVK
                     VKLVLKGILAFEDAKLAADAGIDAIVVSNHGGRVEDGVSATIDVLPEIVGAVGGRMPI
                     LVDSGFRRGSDIVKALALGAQAVCIGRPYLWGLGAFGQPGVERVLGILRAETRTAMQQ
                     LGAPSLKDLTPAMVRRA"
     gene            1926..>2918
                     /locus_tag="VGA15_25180"
     CDS             1926..>2918
                     /locus_tag="VGA15_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591018.1"
                     /translation="MIGAPPEASNIKLVGADDLQARSAYQPTIHHQGDRWIAYIGHHG
                     GTDDIPAPVNPQTEKAEPNGTSIVDVTDPAHPKYLRHIPGQEGKYEAGGAQMVRICDG
                     KALPKGDPNAVYMLRTFGGEAHEIWNVADPANPVLITRIGGLKDTHKSWWECETGIAF
                     LVSGAPDWRTRRMTQVYDLSDPAHPSKIRDFGLPGQEPGATGAVPTELHGPISTGPEG
                     NRVYFGYGTNKGGFLQIVDRDKLINGPKEPTPDNLRLPEISRMPMSAFNGAHTTFPMR
                     GMPIAEFAHDKDGKTRDIVMIVDEAILNECSEPRQMVWFADVTVENRPMMISSFT"
BASE COUNT          564 a          901 c          908 g          545 t
ORIGIN      
        1 cgccgtcggt cagggcgtgc cagagttttt cggcggtggt ctcgatgtag gtgatgtaga
       61 cgaattccgg tttactcatc gcgcttctcc aattgccgtt tcaattcgcc gagcgcggcg
      121 agcttgccgc gctcgaattt cctgatccac cgctcgccga tctgatggat cggcaccggg
      181 ttgaggtagt gcagcttctc gcggccatgc cggaacgtgg tgacgaggtt ggcggcctcc
      241 agaatcgcaa ggtgcttggt gacggcctgc cgcgtcatgg cgaggccgtc gcagagttcg
      301 ttcagggtct ggccgttttt ggcgtgaagc cggtccagca gcgaccgcct tgaagcatcg
      361 gccagcgctt tgaagacctc atccatgacc aggataatag gcaaccaaat ggttgcatgt
      421 caagagggcg tttcgcggtc gatgcgtttc atccgcgcaa gcgacggatt ttgtcgtcat
      481 cgaggaaaag gaactcaggc tcgccggacc atcgccggcg tgaggtcttt gagtgacggc
      541 gcgccaagtt gctgcatggc ggtgcgagtc tcggcgcgca agattccaag aacacgctcg
      601 acacctggct gaccgaaagc gcctagcccc cacagatagg gccggccgat acacaccgct
      661 tgcgcgccca gtgccagtgc cttgacgatg tcgctgccgc gccgaaaacc gctgtcgacc
      721 aggatcggca tacggccgcc gaccgccccg acgatttccg gcagcacgtc gatggtcgcg
      781 ctgacgccgt cttccacgcg cccgccgtgg ttcgagacga cgatggcatc gatgcctgca
      841 tcggcggcga gctttgcgtc ctcgaaggcg agaatgcctt tgagcacgag cttgaccttg
      901 accgtatcgc gcaggcgctt gatcaaatcc caggtcaggt tggtgacgac cgtgctgctc
      961 acgccgccga gatcgatgcc ggtaaaattc ggcttccgcg acagatagtc ggtgaggcca
     1021 tgacagctgc cgcagtcgcg ggcgtcggtg cgccgcaatc ggacgaaggt ctcccatttc
     1081 gcgaatgacc ttacatcgag cgtcaccgcg atcgccggcg cgccggcagc ctccgcccgc
     1141 ttgaccaggc cttcggcaac ctcccagcgc tgcgtcgtat agagctggaa ccataccggg
     1201 gcgccgcgcg cggcgattgc atcctcgatc gatgttgttg cgacggtgga cagaatttgc
     1261 agatggttgc cggctttcgc ggccttggcg acggcgatct cgccatcggc gtgaaaagcg
     1321 cggttgctgc ccgccggcgc aatgacgatc gggctgtcgt atttcgtgcc gaggatttcg
     1381 gcgctgatgt cgattttgct gacatcgacg agccgccgcg gccgcagctc gaatttccgg
     1441 aaaccttcgc ggttggcgcg caaggtcact tcgtcatcgg cccccgtggc catgtagccg
     1501 aaatgcgccg gcggaacatt ctttttcatg accggctcga aatcgaaaac gtcgagcgcc
     1561 tgtgtggggt cggcaatcaa tttgtcgggg tcgcgcggcg cccagtcaac cggatccgat
     1621 ggccgcggcc cttccgccaa ggcgccgcgc gcgaacaacg ggctggcagc caaaaattgc
     1681 agcaaacggc ggcggctggc gaagtcaata tgatgcggcg acatggttgc ctccccaaaa
     1741 gaagagatgt tcgcgatcga ccaagcgtag tgcaatccgg gcgcgcttcg ctatgatatc
     1801 tttcagccag cgaagactgg tttcgataat ctgggtggaa aaacatgacc ggtcagatca
     1861 aactggcaac gtattcttgc atatgtttta tcgcttgctg caccgccgcg tatgcgcagc
     1921 agcaagtgat tggcgctccg ccggaagcgt ccaacattaa gctggtcggg gctgacgatc
     1981 tgcaggcgcg cagcgcctac cagccgacca tccatcatca gggcgaccgc tggattgcct
     2041 atatcgggca tcacggtggc accgacgata tcccggcgcc ggtcaatccg cagaccgaaa
     2101 aggccgagcc gaacggaacc tcgattgtcg acgtcaccga tcccgcacat ccaaagtatc
     2161 tgcggcacat tcccggccag gaaggaaaat atgaggccgg cggcgcgcag atggtgcgca
     2221 tctgcgacgg caaggcgctg cccaagggcg atcccaacgc ggtgtacatg ctgcgcacgt
     2281 ttggcggcga agcgcatgaa atctggaatg tcgcggatcc cgccaatccg gttctcatca
     2341 cccggatcgg gggcctgaag gatacccaca agagctggtg ggaatgcgaa accggcattg
     2401 ccttcctggt gtcgggcgcg cccgactggc gcacgcgccg catgacccag gtctacgacc
     2461 tcagcgatcc tgcgcacccc tcaaaaatcc gcgatttcgg ccttcccggt caggagccgg
     2521 gcgcaaccgg cgcggtgccg accgagctgc atgggccgat ctcgaccggc cccgaaggca
     2581 accgcgtcta tttcggctac ggcaccaaca agggcggctt cctgcagatc gtcgatcgcg
     2641 acaagctgat caacggaccg aaggagccga cgccggataa tcttcgtctg ccggaaattt
     2701 cccggatgcc gatgtccgcg ttcaacggcg cccataccac ctttccaatg cggggaatgc
     2761 cgatcgccga attcgctcac gacaaggacg gcaagacccg cgacatcgtg atgatcgtcg
     2821 acgaggcgat tttgaacgaa tgcagcgagc cgcggcagat ggtgtggttc gccgacgtca
     2881 cggtcgagaa ccgcccgatg atgatttcga gtttcacc
//
LOCUS       DASWGM010000930         7891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1336196,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000930 DASWGM010000000
VERSION     DASWGM010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336196"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VGA15_25185"
     CDS             complement(<1..1011)
                     /locus_tag="VGA15_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9591019.1"
                     /translation="MNISAKPTAIDVEIHGNFIDGHEIEAGQGRMLDVRNPATGAVIA
                     RIPNSTADDIDRAMKSARAAFEGSTWGGMDIRARARLVNRLADAFEANLESLYRLETL
                     NNGRPLNETRAQLSRLPDFFRYFAGLALARRDAVIPVEGSYLNYTLRTPIGIVANCTP
                     FNHPLMIMCKSLAAVLASGCVTVVKPSEYTPLTTLKLAQIFTEAGLPPGVFNVVLGLG
                     QSAGKMLAEHPDINKLVLTGGTEAGRIAGGAAAKVFAHQTMELGGKTPVMVFDDYDID
                     QAVNYAAFGAFIGAGQTCVCASRHIVQASIYDEFVEKLKAKTKAIRIGDPFDPATQLG
                     PVI"
     gene            complement(1127..1762)
                     /locus_tag="VGA15_25190"
     CDS             complement(1127..1762)
                     /locus_tag="VGA15_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX9591020.1"
                     /translation="MLLRDNVYENLRSDILTCRLAPGDDMREQELAERYAVSRQPVRE
                     ALLRLQREHLVTVQPRQGYRVNPISLSDARDLLRFRLVLEPACVAGAIENATPEVLKA
                     LDEFRSFAGDHEDFIAYNRAFHSALAYASGNRRMAAALCDLIGQADRLVRVSLANVIG
                     HDPTQLVAEHVALIEAMQRRDARGAVRLIKAHVLQTEKRVLPALRRNAVIV"
     gene            1919..2698
                     /locus_tag="VGA15_25195"
     CDS             1919..2698
                     /locus_tag="VGA15_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9591021.1"
                     /translation="MNRLFANGTVHATQSGKGPFLFLFHSLLSDRASFDAVVPRLSQS
                     FRVVVPELPGFGGSQAVSGGLAAVADRMAEALTDAAGGDDVFVLGNGYGGFIALQMAV
                     RHPDLATKLILADCGAAFSEEGRAAFRNMAAASQAKGLSAITDVAMRRLFAPEFQIQH
                     PDLMRDRREAFLRTDPDVFRAACDALAELDLRPELTKVKVPVLVLVGEHDEATPPPMS
                     RELAAGLPHAHLKIIPGCAHVPQLQSPEVFLEAIGDFLPAL"
     gene            2824..3309
                     /locus_tag="VGA15_25200"
     CDS             2824..3309
                     /locus_tag="VGA15_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9591022.1"
                     /translation="MHTVLDYCTGGIERSLSVGTVVIREGETTDHLYVLIEGRLEVVK
                     GDTVVAIITEPGAVLGEMSVLLDQSHTATVRAASDSTIYEISDAASFLREQPAVALLV
                     ARLLAQRLNVATTYLADIMQQYAGHGDHLAMVGEILQSMINLPPIKISPGSDRQSDPR
                     M"
     gene            complement(3263..4075)
                     /locus_tag="VGA15_25205"
     CDS             complement(3263..4075)
                     /locus_tag="VGA15_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX9591023.1"
                     /translation="MRCLIVADLHYSLPQFDWLLSAAPQFDLVIFAGDALDIGSAVDF
                     RAQIVVVKKYLALLAGLTRVILCSGNHDLDERNPEGEKIARWIGDVRALGIACDGDSL
                     TIGDTLFTVCPWWDGPLVKQRIDDQLREAAAARLQRWIWVHHAPPANSPTSWGGKRFF
                     GDVELLQWIQSYQPSMVISGHVHQSPFIPDGSWFDRLGGTWVFNPGLQPGRPPAHIVL
                     DIDSNTAYWLPLGDEQCIDLNAPLQRPAAPISDPPAWLTSLGRIADPNLARS"
     gene            complement(4273..7791)
                     /locus_tag="VGA15_25210"
     CDS             complement(4273..7791)
                     /locus_tag="VGA15_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX9591024.1"
                     /translation="MNNAEFQLQGVSDPRLAVHATSALPAWLWSIDGTRILWANPVGA
                     RLFDAANGAALAKKTFGPADSHRRQVARLASRLPSNAAIRLERLRGFGATFGTLATCG
                     CARLDFADGGHGILVVGAAKAAGRTMPLVERLQRLVEGIDAPMAALTRDGLFVGASDA
                     ARSLLGFRNLSEVGLDGARSDALQHGRVETAVGIGRLILQRVGTGADVGLVALIAPGA
                     TLAAHVRHVAPASPETARAEAARQPVAPTRDLPMPDNEQSAMTGEAAAEVALIDEFAE
                     QPAEAAVEHSSTDAPPEPDLPRAEVSATSMTPEIEVHQDEPSPYVEAVADEPAAARGE
                     DLPSKDLPSKDLPPAVDPTSPAIAPEPASAPSWPDEPSPSPPEHLLRFLWRINAEGRF
                     SLDPSEFTRLIGARTAARFERLMSDIAGLSGLDPDGRFVNAVATHDTWSGITLNWPVD
                     GGDPLPVELSGLPITDSAANFAGYRGFGVCGDLDGLAQLAALRRDESFSGPSAPQMQM
                     QMDPAVIVQPNSADIVATGFGDSPAAELPAKPDVASSDTPPSPNAFETSQQTDLETAV
                     ETPKETPKETPTETPKNVLPFRPISDPKSPALTSVENSAFNELARQLSARLDSENGGV
                     AATPATPDAAETVDGPPSTPTPEPPSDQPAIEQPEWLAPPEAPARGEARRDKPLFDLL
                     PVGVLIYRLDRLLYANRPFLEQMGYESLHALEEAGGLDALYVAPGVSSASSTSDTGRP
                     VTISGTEASSEGAPAIDARLYTISWDGDSALALIFSGTRNEAAAAVAVLPEFIPFRAP
                     SAVGHANAEELGVILDTTAEGIVMFDVEGNIHSCNRSAEALFGYDGTELVQRNLIQLF
                     APESQPAVLDYLKGIKGADVASLLDHGRDVLGRVRKGGIIPLSMTMGRTRPDGPNFFT
                     VFRDVSQSRKSEGELPQAGRPADRAADAKAEMLARISHEVRTPLNAIIGFAEVMIGER
                     FGKLGNARYIEYMKDIRSSGEHVIAIINDLLDLSRIETGRLDLTFASQNLNDLVEQCV
                     AVMQPQANRERIIIRTSLAQMLPPVIADARALRQIALNLIGNSIHLANAGGQVIVSTA
                     LSDFDEVVLRVRDSGQGLNDNEVAAALEPFRTQAPSDQAPDGSGVNLSLTKALVEANR
                     AHFHIKTASHSGTLIEVTFSHARAKA"
BASE COUNT         1396 a         2440 c         2598 g         1457 t
ORIGIN      
        1 gatcaccggc ccgagttggg ttgcgggatc gaagggatcg ccgatccgaa tggctttggt
       61 cttcgccttc agcttctcga cgaattcgtc gtagatcgac gcctgcacga tatggcgcga
      121 ggcgcagacg caggtctgcc cagcgccgat aaaggcgccg aacgccgcat agttgacggc
      181 ctgatcgata tcgtagtcgt cgaacaccat gaccggcgtc ttgccgccga gctccatggt
      241 ctgatgcgcg aacactttcg ccgcggcacc cccggcaatg cggccggctt cggtgccgcc
      301 ggtcagcacc agcttgttga tatcgggatg ttcggccagc atcttgccgg cgctctggcc
      361 gaggccgagc acgacgttga acacgccggg cggcagcccc gcttcggtga agatctgcgc
      421 cagtttcagg gtcgtcaacg gggtatattc cgacggtttt acgacggtga cgcatccgct
      481 cgccagcacc gccgcgagtg acttgcacat gatcatcaag gggtgattga acggcgtaca
      541 gttggcgacg atgccgatcg gcgtgcgcaa ggtgtagttc agataggagc cctcgacggg
      601 gatcaccgcg tcgcggcgcg ccagcgccaa tccagcaaag taccgaaaaa agtccggcag
      661 ccgcgacagc tgtgcgcggg tctcgttgag cggacggccg ttgttgaggg tctccagccg
      721 gtacagcgat tccagattgg cctcgaaggc atccgcgagg cgattgacca gccgtgcgcg
      781 ggcacggatg tccatgccgc cccaggtgct gccttcgaac gcagcccgcg cgcttttcat
      841 cgcgcggtcg atgtcatcgg cggtcgaatt cggaatccgc gcgatcacag cgccggtggc
      901 gggattgcgg acatccagca tccggccttg gcctgcttcg atctcgtggc catcaataaa
      961 gttgccgtgg atttcgacat cgatggccgt tggcttcgct gaaatgttca tgggtctctc
     1021 ccttggatcg tgatccactc aattaaatcg gatcacgatc tcatctttag tgtttgagca
     1081 tggatctccg cgcaaacgct tcgcgtttgt cgcgagggat catgctctag acgatcaccg
     1141 cgttgcgcct gagcgccggc agcacccgtt tttcggtttg cagaacatgc gccttgatga
     1201 gacgcacggc gcctcgcgca tcgcgccgct gcatcgcctc gatcagcgcg acgtgctcgg
     1261 ccaccagttg cgtcgggtcg tggcctatca cattggcgag gctgacgcgc accagccggt
     1321 cggcctgtcc gatcagatcg cacagcgccg cggccatgcg gcggttaccc gacgcatacg
     1381 ccagcgcgga gtggaaggcg cgattgtagg cgatgaagtc ctcgtggtcg cccgcgaaag
     1441 agcggaattc gtccagcgcc ttcaaaacct ccggcgtggc gttctcgatc gctcccgcga
     1501 cgcaggcggg ctccagcacc agccggaacc gcaacaggtc gcgggcatcc gaaagcgaga
     1561 tcggattgac ccggtaaccc tggcgcggct gcaccgtgac cagatgctcg cgttggagcc
     1621 gcagcaacgc ctcgcgcacc ggctggcggc tgaccgcgta acgttcggca agctcttgtt
     1681 cccgcatatc atcgccgggc gccaaacgac atgtcagaat atcagaccgc aggttttcat
     1741 agacattgtc gcgcaaaagc atcgaaattt ccttgagtat gcaggccagt ctggcagtcg
     1801 tgaaatatca cgtcaagtta aatgacttga aaaggccgaa ttttgatcta tcaatccgaa
     1861 cgcgccgacg agcgccgcct cgccatgcgg tgcgctccca acctgaaaga gagccggcat
     1921 gaaccgactt ttcgccaacg gcaccgtcca tgcgacgcag tccggcaagg ggccgtttct
     1981 gtttctgttt cattcgctgc tgtcggaccg ggccagtttc gacgccgtcg ttccccgact
     2041 atcgcagtcg ttccgggtgg tggttccgga gttgccgggc ttcggagggt cccaggcggt
     2101 cagcggaggc cttgccgcgg tcgccgaccg aatggccgag gcactgacgg atgcggccgg
     2161 cggcgacgat gtgttcgtgc tcggcaatgg ctatggcggg ttcatagcgc tgcaaatggc
     2221 cgttcgccat cccgacctcg caaccaaact cattctcgcc gattgcggcg cggcgttttc
     2281 ggaagaaggc cgcgcggcct ttcgtaacat ggccgcggca tcccaggcca aggggctgtc
     2341 ggcgattacc gacgttgcga tgcgccggct gtttgcgccg gaatttcaga tccagcatcc
     2401 cgacctgatg cgcgaccgtc gcgaggcgtt tttgagaacc gatccggacg tattccgcgc
     2461 cgcctgcgat gcgctggccg aactcgacct ccggcccgag cttacgaagg tcaaggtccc
     2521 cgttctggtc ctggtcggcg agcatgatga ggcgacgccg ccaccgatgt cccgcgaatt
     2581 ggccgctggc ctgccgcacg cgcatctgaa gatcattccg ggctgcgcgc atgtcccgca
     2641 gctgcaatcg cccgaggttt tcctggaggc gatcggggat tttttgccgg cgttgtaagg
     2701 tgcgtaatac ggcaggcccg actttgctcg atgcaactaa tttgagcaga ccgaacgcat
     2761 tatccgttcg acgatagcag ccctctttgc ctagtcttgc ccgaggcttg gggtatttgc
     2821 cggatgcaca cggttctgga ctattgtacc gggggaattg agcgaagtct gtccgtcggc
     2881 acagtcgtga tccgggaggg ggagacgacc gatcacctct atgtcctgat cgagggacgg
     2941 cttgaggtcg tcaagggcga tacggttgtc gccatcatca ccgagccggg agcggtgctg
     3001 ggagagatgt cggttctgct cgatcagtcg cataccgcga ccgtgcgcgc ggcgtctgat
     3061 tcaacaatct acgagatcag cgatgccgca tcgtttctgc gcgagcagcc ggcggtggcg
     3121 ctcttggtgg cgcggctgct ggcccagcgg ctcaatgtcg ccaccaccta tcttgccgac
     3181 atcatgcagc aatatgccgg ccacggcgat catctggcga tggtgggcga gattctacag
     3241 agcatgatca acctgccgcc gatcaagatc tcgccaggtt cggatcggca atccgaccca
     3301 aggatgtaag ccacgccggc ggatcgctga tcggcgcggc gggccgctgc aacggcgcgt
     3361 tgaggtcgat gcattgctcg tcgccgagcg gcagccagta cgcggtattg ctgtcgatat
     3421 cgagcacgat atgcgccggc gggcggcccg gttgaaggcc ggggttgaac acccaggtgc
     3481 cgccgagccg atcgaaccag gagccgtcgg ggatgaacgg cgattggtgc acatggccgg
     3541 agatcaccat cgaaggctga tagctctgga tccactgcag gagttcgacg tcgccgaaaa
     3601 agcgcttgcc gccccaactg gtcggcgaat tcgccggcgg cgcgtgatga acccagatcc
     3661 atcgctgcag ccgcgccgcg gcagcctcgc gcagctggtc gtcgatccgc tgcttgacca
     3721 gtggcccgtc ccaccacgga cagaccgtga atagggtgtc gccgatcgtc aggctgtcgc
     3781 cgtcgcaggc gatgccgagc gcgcggacgt cgccgatcca acgggcgatc ttctcgcctt
     3841 cgggattgcg ttcatcgagg tcatgatttc cggagcaaag aatgacccgg gtgagaccgg
     3901 cgagcagcgc cagatatttc ttcacgacga caatctgggc gcggaaatcg accgcggatc
     3961 cgatgtcgag ggcatcgccc gcgaagatca cgagatcgaa ttgcggggcc gcgctgagca
     4021 accagtcgaa ctgcggcagc gagtaatgca agtcggccac gataaggcag cgcatttcaa
     4081 atcgatccgg atgctcaaga actgggcgac gcgggcgggc ttgtattcaa ccagccagga
     4141 tcgagcaaaa attgtgccgt gcctgcttgt tgaaatgcga cagatgcccg catgaaccgt
     4201 tctcgtggtg gccgggctta tggcttttta agcgagagga cgccggcacc catcaagaaa
     4261 accgggagga ttttacgctt tcgctctggc gtgggaaaac gtcacttcga tcagcgtgcc
     4321 ggaatgcgaa gcggtcttga tatgaaaatg cgcgcggttg gcttcgacca gggccttggt
     4381 cagggaaagg ttgacgccgg aaccgtcggg cgcctggtct gacggtgcct gggtccggaa
     4441 cggctccagc gcggccgcga cctcgttatc gttgagcccc tgtccgctgt cgcggacccg
     4501 cagcaccacc tcgtcgaaat ccgacagcgc ggtggagacg atgacctggc cgccggcatt
     4561 ggcgagatgg atcgaattgc cgatcaggtt cagcgcgatc tggcgcaacg cgcgggcgtc
     4621 ggcgatcacc ggcggcagca tctgcgccag cgacgtccgg atgatgatgc gttcgcgatt
     4681 ggcctgcggc tgcatcaccg cgacgcattg ttcgaccaga tcgttgaggt tctggctggc
     4741 aaaggtgagg tcgagcctgc cggtttcgat ccgcgacaga tcgagcagat cgttgatgat
     4801 ggcgatcacg tgttcgccgg aagagcggat gtccttcatg tattcgatgt agcgcgcgtt
     4861 gccgagcttg ccgaaacgct cgccgatcat cacttcggca aagccgatga tggcgttgag
     4921 cggggtgcgg acctcgtggc tgatccgcgc cagcatctcg gccttggcgt ccgccgcgcg
     4981 atcggccggt cgccccgcct gcggcaattc accctcgctt tttctgctct gcgagacatc
     5041 gcgaaacacc gtgaagaaat tcgggccgtc gggccgggtg cgtcccatgg tcattgacag
     5101 cgggatgatg ccgcccttgc ggacccggcc cagcacgtcg cggccgtgat cgagcaggct
     5161 cgcaacatcg gcgcccttga tgcctttcag ataatccagc accgcaggct ggctttccgg
     5221 cgcgaacagc tgaatgagat tgcgttgcac gagctcagtg ccatcgtagc cgaacagcgc
     5281 ctcggcgctg cggttgcatg aatggatgtt gccctcgacg tcgaacatca cgatgccttc
     5341 ggctgtggtg tcgaggatga cgccaagttc ctcggcattg gcgtggccga cggccgacgg
     5401 tgcgcggaag gggatgaatt cgggaagcac ggcgaccgcg gcggcggcct cgttgcgcgt
     5461 gcccgagaaa atcagagcca gcgcggaatc gccgtcccaa gaaatcgtgt acaggcgcgc
     5521 gtcgatcgcc ggcgcacctt cggaggaagc ctccgttccc gaaatcgtca ccggcctgcc
     5581 ggtgtccgat gtgctgctgg cgctggagac gccgggcgcg acatagagcg cgtcgagacc
     5641 tccggcctct tccagggcat gcaggctctc gtagcccatc tgttcaagaa acggacggtt
     5701 ggcgtagagc agccggtcaa gccgatagat cagcacgccc acgggcagca aatcgaacag
     5761 cggcttgtcg cgccgggctt cgccacgcgc gggcgcctcg ggaggcgcca gccattcggg
     5821 ttgttcgatc gcgggttgat cgctcggcgg ttcgggtgtt ggcgtcgacg gcggcccgtc
     5881 gacggtttcg gcagcgtcgg gcgttgccgg cgtcgcggcg acaccaccgt tttcgctgtc
     5941 gagccgcgcc gacaattgcc gggcgagttc gttgaaggcg ctgttttcca ccgacgtcag
     6001 ggccggggat ttcgggtcgc tgatcggccg aaacggaagg acgttcttgg gcgtttccgt
     6061 gggcgtttcc ttcggagttt ccttgggcgt ttccacagcg gtttccaaat cggtttgttg
     6121 tgaggtctcg aaagcattcg gcgatggagg cgtatcggac gatgcaacat caggcttcgc
     6181 cggcagctcc gcggcaggcg aatctccaaa acctgtcgcc acgatgtcgg ccgaattggg
     6241 ctggacgatg acggccggat ccatctgcat ctgcatctgc ggcgccgacg gaccgctgaa
     6301 agattcgtcg cggcgcaacg cggccaactg cgccaggccg tcgagatcgc cgcacacgcc
     6361 aaaacctcga tagccggcaa aattcgccgc ggaatcggtg atcggcaatc ccgacaactc
     6421 caccggcaat gggtcaccgc catcgacggg ccagttcaag gtgatgccgc tccaggtatc
     6481 atgggtcgcg accgcgttga cgaagcggcc gtcgggatcg agccccgaca atccggcgat
     6541 gtcgctcatg agccgttcga agcgagccgc ggtgcgcgcg ccgatcaggc gggtgaattc
     6601 gctcgggtcg agcgagaaac ggccttcggc attgatccgc cacaggaagc gcagcaggtg
     6661 ctcgggcggg ctaggcgagg gctcatcggg ccatgacggc gccgaggcgg gctccggtgc
     6721 gatggctggt gaggtggggt cgacggccgg aggcaggtct ttcgaaggca ggtctttcga
     6781 aggcaggtct tcgccgcgcg cagctgcagg ctcgtccgcg accgcttcga catagggaga
     6841 aggttcgtcc tgatgaacct cgatctcggg cgtcatcgat gtcgccgaga cttcggcgcg
     6901 cggtagatcg ggttcgggcg gcgcgtccgt cgaggagtgc tcgacggcgg cctctgcggg
     6961 ctgttccgcg aactcatcga tcaacgcaac ctccgccgcg gcttcgcccg tcatcgcgga
     7021 ctgttcgtta tcaggcatcg gcagatccct cgtcggtgcg actggctggc gcgcagcttc
     7081 ggccctggcg gtttccggcg atgccggtgc aacatgcctg acatgggcgg ccagggtcgc
     7141 gccgggcgca atcagggcca cgaggccgac atcggcgccg gtaccgacac gttgcagaat
     7201 caaacggccg atgccgacag ccgtctcgac gcggccgtgc tgcaaggcgt cgctgcgcgc
     7261 gccgtcgagg ccgacctcgg agagattgcg aaagccgagc agcgaccgcg cagcgtcgct
     7321 ggcgccgacg aacaggccgt cgcgcgtgag cgccgccatc ggcgcatcga tgccctcgac
     7381 cagccgctgc aaccgctcga ccagcggcat ggtgcggccg gccgccttgg cggcacccac
     7441 caccaggatg ccgtggccgc catcggcaaa atcgagccgc gcgcagccgc aggtcgcgag
     7501 ggtgccgaac gtggcgccga agccgcgcag ccgttccagg cggatcgccg cgttggatgg
     7561 cagccggctc gccaaccgcg ccacctggcg ccggtgtgaa tccgccggcc cgaaagtctt
     7621 tttggcaagc gctgcgccat tggcggcgtc gaacagcctg gcgccgaccg gattggccca
     7681 caggatccgc gtgccgtcga tcgaccacag ccatgcggga agggcgctcg ttgcatgcac
     7741 cgccagtcgc ggatcgctga cgccctgcaa ctggaattcc gcattattca tcgcttggaa
     7801 tgaccggctg gcttggctgt tgctcgacga cagaatcggc ggccaaacga taacagcctt
     7861 taacggattg ttagtattgc ctgcaggaga c
//
LOCUS       DASWGM010000931         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1477116,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000931 DASWGM010000000
VERSION     DASWGM010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477116"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VGA15_25215"
     CDS             complement(<1..531)
                     /locus_tag="VGA15_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270579.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX9591025.1"
                     /translation="MRAEGIETARRILCVFPRYTSSFGTFEYAYPLTDGVQAFMPPQG
                     LLLIAAYLPANWSVRFIDENIRATTAEDFEWAEAVFVSGMHIQRQQMTDICRRAHAFD
                     LAVAIGGPSVSACPDYYPSFDYLHVGELGDATDDLIARLARDPSRPAQQVVLKTADRL
                     DMTEFPIPAYELADVKR"
     gene            complement(740..>1697)
                     /locus_tag="VGA15_25220"
     CDS             complement(740..>1697)
                     /locus_tag="VGA15_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEX9591026.1"
                     /translation="VTSAFRNTIGLRGRMSVRLQPNHPFDDAKGITASILDGILLGSG
                     DACIGINPASDDPALIGGLWRLLDDIIVRLRIPTQSCILSHVTTTLGLIEQGVPVDLV
                     FQSIAGTQAANRSFGIDLALLREAHQAGLSLRRGTVGNNVMYFETGQGSALSANAHHG
                     VDQQTCEARAYAVARAFDPLLVNSVVGFIGPEYLYDGKEIIRAGLEDHFCGKLLGLPL
                     GVDVCYTNHAEADQDDMDNLLTLLAAAGVTFIMGVPGADDVMLNYQSTSFHDALYIRD
                     LFGLKRAPEFDDWLFRAGLADAEFRLAGNTGVLPDFASRLIA"
BASE COUNT          345 a          526 c          531 g          295 t
ORIGIN      
        1 gcgtttgacg tcggcgagct cataggccgg gatcgggaat tcagtcatgt ccaggcggtc
       61 ggcggtcttc aggaccacct gctgggcggg acgggatggg tcgcgcgcca gccgcgcgat
      121 caggtcgtcg gtcgcgtcgc cgagctcgcc gacatggaga tagtcgaacg atggatagta
      181 atccgggcag gcgctgaccg agggtccgcc gatcgcaacc gccagatcga acgcatgcgc
      241 ccggcggcag atatcggtca tctgctgacg ctgaatatgc atgccgctga cgaacaccgc
      301 ttccgcccat tcgaaatcct ccgccgtcgt ggcgcggatg ttttcgtcga tgaagcggac
      361 cgaccagttc gccggcaaat aagccgcgat cagcagcaga ccctgcggcg gcatgaaggc
      421 ctggacgccg tcggtcaggg gataggcgta ctcgaacgtt ccaaaagaag atgtatagcg
      481 cggaaaaacg cacaggatgc gccgtgccgt ttcaataccc tcagctctca tcaaatttcc
      541 tctaagtcct cgggcaaggg taatattgca caaattaagc acgaccccca aggccgagcc
      601 aaggctgagc tacaaattcc tcaacattgt gatagccgct ccaacgctgg ggtaaccgga
      661 tggttgcgcg ccagcgtccg aaatccgaca ttcgcgcctc cttgccgtgt ttcccggggc
      721 ggcggccttt tcggttccct caggcgatca atcgggacgc aaaatcgggc agcaccccgg
      781 tgttgccggc gagccgaaac tcggcgtcgg ctaacccagc gcgaaacagc caatcgtcga
      841 attccggcgc ccgcttcagg ccgaacagat cgcggatata aagtgcgtcg tggaacgaag
      901 tggactggta gttcaacatg acgtcgtcgg ccccgggcac ccccatgatg aaggtgacgc
      961 cggcggcggc gagcagcgtc aacagattgt ccatgtcgtc ctggtcggct tcggcgtggt
     1021 tggtgtagca gacgtcgacg ccaagcggca ggccgagcaa cttgccgcaa aaatggtcct
     1081 cgagcccggc gcgaatgatc tcctttccat cgtagagata ttccgggccg atgaagccga
     1141 ccacgctgtt gaccaatagc ggatcgaacg cgcgcgccac cgcgtaggcg cgcgcttcgc
     1201 aggtctgctg gtcgacaccg tgatgggcgt tggccgaaag cgccgagccc tgcccggtct
     1261 caaaatacat cacattgttg ccgacggtac cgcgccgcaa cgacagcccg gcctggtggg
     1321 cttcccgcag aagagcaaga tcgatcccga aactccggtt ggcggcttgg gtgcctgcga
     1381 tcgactgaaa caccagatcg accggcacgc cctgctcgat caatccgagc gtcgtcgtca
     1441 cgtgtgagag gatgcagctc tgcgtcggaa tccgcagccg tacaatgatg tcatcgagca
     1501 gccgccagag cccgccgatc aaggccggat cgtcgctcgc cggattgatg ccgatgcagg
     1561 cgtcgccgga tcccagcaga atgccgtcga ggatcgaggc ggtgatgccc ttggcgtcat
     1621 cgaagggatg gttgggctgc aaccgcacgc tcatccgccc gcgcagccca atcgtgttgc
     1681 ggaaggccga tgtcacc
//
LOCUS       DASWGM010000932         1850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1336587,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000932 DASWGM010000000
VERSION     DASWGM010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336587"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1230
                     /locus_tag="VGA15_25225"
     CDS             58..1230
                     /locus_tag="VGA15_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX9591027.1"
                     /translation="MIPNAHRMFNFDLGETADAIRETVHDFSSNEIAPRAAEIDKSNQ
                     FPRDLWPKLGALGLHGITVEEEYGGSGLGYLEHCIAVEEISRASAAVGLSYGAHSNLC
                     VNQLRRNGNEAQKRKFLPKLISGEHVGALAMSEPGAGSDVVSMSTRAEKKGDRYVING
                     SKMWITNGPIAETLVVYAKTDPAAGSRGITAFIIEKGMKGFSTAQKLDKLGMRGSDTG
                     ELVLENCEVPEENVLGEVGRGVNVLMSGLDYERSVLAAGPLGIMQACLDVVMPYVHER
                     KQFGEPIGSFQLVQGKIADMYTTMNASRAYVYAVAKACDRGETTREDAAGAILYASEK
                     ATQCALDAIQLLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFEKSA"
     gene            1332..>1850
                     /locus_tag="VGA15_25230"
     CDS             1332..>1850
                     /locus_tag="VGA15_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HEX9591028.1"
                     /translation="MSFVPPHPPPMPVSFSGDRGEFFGLVTRGAGLELITVGFYRFWL
                     ATDIRRHLWSNTHIDGDAAEYTGRGRELLIGFLIALAILVPIYLAYFLVGLEAERYKA
                     FASIPLFLFFYLFGQFAIYRARRYRLTRTVWRGVRFWMSGSGWAYAGQAALWGLLMIL
                     TLGLILPWRGAAL"
BASE COUNT          353 a          547 c          580 g          370 t
ORIGIN      
        1 ccagagaata aggctgtgca cggaaagcag cgttatttga ccggaggaca cccgcccatg
       61 atccccaacg cgcatcggat gttcaatttt gatcttggcg aaactgcgga tgcgattcgc
      121 gagaccgtgc atgatttctc cagcaatgag atcgcgccgc gcgccgccga aatcgacaaa
      181 agcaatcaat tcccgcgcga tctctggcca aaactcggtg cgcttggcct gcacggcata
      241 accgtcgagg aagagtatgg cggatcgggc ctgggctatc tcgagcactg catcgcggtc
      301 gaggagatct caagggcgtc ggcggcggtc ggcctgtcct acggcgcgca ttcgaacctt
      361 tgcgtcaatc agttgcgccg caatggcaac gaagcgcaga agcgaaagtt tcttccaaaa
      421 ctgatttccg gtgaacatgt cggggcactg gcgatgtcgg agcccggcgc cggttccgac
      481 gtggtgtcga tgtcgacccg cgccgagaag aagggcgacc gctacgtcat caacggcagc
      541 aagatgtgga tcaccaacgg cccgatcgcg gaaaccctgg tggtctatgc caagaccgat
      601 cccgccgccg gctcgcgcgg catcaccgcc ttcatcatcg aaaagggcat gaagggattt
      661 tcgaccgcgc agaagctcga caagctcggc atgcgcggct ccgacaccgg cgaactggtg
      721 cttgagaatt gcgaagtgcc ggaggaaaac gtgctcggcg aggtcggccg cggcgtcaat
      781 gtgctgatgt ccggcctcga ctacgagcgc tcggtgctgg cggcggggcc gctcgggatc
      841 atgcaggcct gcctcgacgt ggtgatgccc tatgtccacg agcgcaagca gttcggcgag
      901 ccgatcggca gcttccagct ggtgcagggc aagatcgccg atatgtacac cacgatgaat
      961 gcgtcccgcg cctacgtcta tgccgtcgca aaagcctgcg accgcggcga gaccacgcgc
     1021 gaggatgcgg ccggcgccat tctctatgcc tcggaaaagg cgacacaatg cgcgcttgac
     1081 gcgatccagc tgctcggcgg caacggctat atcaacgact atcctacggg gcggctgctg
     1141 cgcgacgcca agctttacga gatcggggca ggcaccagcg aaatccggcg catgctgatc
     1201 ggacgggagc tgttcgagaa atcggcataa aattcggtcg aaccggatgg tattttgagg
     1261 cgacaaaacc ggtaatatgt taattcctgc ccgccgattt acttgccttg gggagttgat
     1321 ccgtgaatca gatgagtttt gtgccgccgc atccgccgcc gatgccggtg tcgttttcag
     1381 gcgatcgcgg tgaatttttc ggactggtga cgcgcggcgc cgggctcgaa ctcatcaccg
     1441 tcggttttta tcggttttgg ctcgccaccg acattcgccg ccatttgtgg tcgaacaccc
     1501 atatcgacgg cgatgcggcg gaatacaccg gccgcggtag ggagttgctg atcggatttc
     1561 tgatcgcgct ggcgatcttg gtgccgatct atctcgccta cttcctcgtc ggccttgaag
     1621 cggaacgcta caaggcgttc gccagtattc ccttgttcct ctttttctat ttgttcggcc
     1681 aattcgcgat ctaccgcgcg cggcgttatc gcctgacccg cacggtgtgg cgcggggtgc
     1741 ggttctggat gagcggttcg ggctgggcct atgcggggca agccgcactc tggggcctgc
     1801 tgatgatact caccctcggc ctgatactgc cgtggcgtgg agccgccctc
//
LOCUS       DASWGM010000933         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_519610,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000933 DASWGM010000000
VERSION     DASWGM010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519610"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VGA15_25235"
     CDS             complement(<1..352)
                     /locus_tag="VGA15_25235"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HEX9591029.1"
                     /translation="MKPLGRATRKHPPSQIRKLQASIEQFGFVLPVVIDVDSRVIAGW
                     GLAVAAKRLGLTEVPAVTIADLDEAKLRMLRLALNRLGEDSGWDLDALKLEFSDILEI
                     SSEIDLRISGFEMGE"
     gene            complement(590..>1625)
                     /locus_tag="VGA15_25240"
     CDS             complement(590..>1625)
                     /locus_tag="VGA15_25240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591030.1"
                     /translation="HQVERIERKPASPRWGWSGVQKIVPPLWAEAELRGVAGLLPSRQ
                     SSIEYIVAELLTIGGRYHRTLHQDEFGPTRAERMQALRGLVDHLEVLLSRLEALPPQL
                     RLLVSEQQTEGRCAAGPIDGDPIASYCADKDAIEVVLEIASDVRRDLAGAGRTDDANL
                     IGEVWAAADMVVPLLWNLDSTTDGDVVIDGESAGSALADNSADPFASLCAPVRRLHSR
                     FKLALIHLGRRKGPEPRLSLDLLVWQLCDLWQRETGRPVTANPVKLGVYTGRPQSASG
                     RFVCAAVEALQPSAAWIDEHKAVEPHVRAAIMMLPPGHRVQPVHSAMRAYVADRRDPS
                     APRRGRPRRK"
BASE COUNT          310 a          506 c          508 g          301 t
ORIGIN      
        1 tctcgcccat ctcaaagccg ctgatgcgaa ggtcgatctc gctgctgatc tccagaatgt
       61 cggagaactc cagcttcagc gcatcaagat cccagcccga gtcctcgcca agccggttga
      121 gcgcgagccg cagcatccgc agtttggcct cgtcgaggtc ggcgatggtc acggcgggga
      181 cttcagtcag gccgagcctt ttggcggcaa cagccaatcc ccaaccggcg atcacgcggc
      241 tgtccacgtc gatgacgacg ggaaggacga agccaaactg ttcgatgctc gcctgcagct
      301 tgcggatctg tgagggcggg tgtttgcgcg tcgctcggcc gagcggcttc aaggatgcaa
      361 cgcggacgag atttggattg agattgcggg atacggcaaa gccgttcccg cccagcatgg
      421 gctgccggtt catcgggtat ctccttcaaa agggccaggc gcgaatcact tcgggccaaa
      481 tatcatgggc ttagctgtcc ggcgggaagt gtggcccatg cgcggtggat aggtgcgatt
      541 cgacgtggtc acgtcatacc atcggaccgc accagccgaa tttacaaagt tactttcggc
      601 gagggcgccc acgccgcggc gccgacgggt cgcgccgatc ggctacgtaa gctcgcattg
      661 ccgaatggac gggttgaacc cggtggcctg gaggcagcat catgatcgcg gcgcgcacgt
      721 ggggttcgac tgccttgtgt tcgtcaatcc aagcggcgct cggttgcaac gcttcaaccg
      781 ccgcgcacac gaagcgtcca gatgccgatt gtgggcggcc cgtgtagacg ccgagcttga
      841 cgggattggc ggtgacgggc ctgccggtct cgcgttgcca gagatcgcac agttgccaga
      901 ccagcagatc caatgacagg cgcggctccg gacctttccg acgcccgagg tggatgagcg
      961 caagcttgaa ccggctgtgc agacgcctga ccggcgcgca tagggacgcg aagggatcgg
     1021 cagagttgtc cgccagagcg gaaccggctg actcgccgtc gatgacgaca tctccgtccg
     1081 tggtcgagtc gagattccac aggagcggca ccaccatgtc ggcggcggcc caaacctctc
     1141 caatcaggtt ggcatcgtcg gtcctaccgg cgccggccaa gtcgcggcgg acatcagacg
     1201 cgatctccaa cacgacttca atggcatcct tgtcggcgca ataggacgcg atggggtcgc
     1261 cgtctattgg ccctgccgcg cagcggcctt ccgtctgctg ttccgatacg agcaaccgca
     1321 gctgcggcgg cagagcttca agccgcgaca acaggacctc cagatgatca acaaggcccc
     1381 gcagcgcttg catccgctct gcccgggtcg gcccaaactc gtcttgatgc agggtgcgat
     1441 gatagcggcc tccgatagta agtaactctg caacgatgta ttcaatcgat gattgacggc
     1501 tcggcagtag accagcgacg cccctcaact ctgcttccgc ccataatgga ggaacgatct
     1561 tctggacccc actccagccc catcgtgggc tcgcgggttt tctttcaata cgttcgactt
     1621 gatgt
//
LOCUS       DASWGM010000934         5638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_130374,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000934 DASWGM010000000
VERSION     DASWGM010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130374"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VGA15_25245"
     CDS             <1..1201
                     /locus_tag="VGA15_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX9591031.1"
                     /translation="VAGWFVPMVIVVALVAFGAWALFGPEPKMAFGLVAAVSVLIIAC
                     PCALGLATPMSIMVGVGRGAQAGVLIKNAEALERMEKIDTLVVDKTGTLTEGKPKVVS
                     IIATARFQESEILRLAASVERASEHPLADAIVRAAKEHNLELSKVDEFFSPTGKGATG
                     KVDGKTILLGNSNFLTSVGIETQSLNEQGERLRGDGATVINIAVDGKLAGLFAIADPV
                     KPSTLDALKTLAAEGIKVIMLTGDNRTTANAVARKLGISDVEAEVLPDQKSAVVTKLQ
                     KAGRIVAMAGDGVNDAPALAAADVGIAMGTGTDVAMESAGITLLKGDLGGIVRARRLS
                     QATMGNIRQNLFFAFIYNAAGIPIAAGILYPHFGILLSPIIAAAAMALSSVSVVGNAL
                     RLRVTRL"
     gene            1257..1682
                     /locus_tag="VGA15_25250"
     CDS             1257..1682
                     /locus_tag="VGA15_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX9591032.1"
                     /translation="MANFTPVSAAIGGGLIGLSAVLLMLLTGRIAGISGIFGGLLNFK
                     SDDRDWRIVFIAGLILAPLIAGWIGYAMPTPKLPASWTVIIGAGLLVGFGTRLGGGCT
                     SGHGICGIARLSSRSIAATVIFMLAAIATVAVTHHLLGG"
     gene            1686..2141
                     /locus_tag="VGA15_25255"
     CDS             1686..2141
                     /locus_tag="VGA15_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX9591033.1"
                     /translation="MRMIASLVCGLVFGAGLLISGMVQPTKVLGFLDIFGAWDPSLAV
                     VMAAALAVSTPGFMLAKGRSRPLLATKSFWPTKRDIDRPLVVGAALFGLGWGLVGLCP
                     GPALENLVTLSPGIIVFVAAMAGGMALHDFWQNSQPKIRNREALANADG"
     gene            complement(2165..3112)
                     /locus_tag="VGA15_25260"
     CDS             complement(2165..3112)
                     /locus_tag="VGA15_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX9591034.1"
                     /translation="MTRVQDVYPADRVSLREVGLRDGLQLVKTFPSTAAKQRWLRDEY
                     AAGVRHFEVGSFLPAKTFPQFADVRDVVKAVASLPGAHGIALALNERGVNEALASGVA
                     EIASVVSATEEHSQANANRSREEAIANVRRLCELRDSSPHKPIVNAAISMALGCSIAG
                     PVDPKEVIKLAERCLEAGVDFVAVADTVGYAGPKQVGELTKAIVNISGAKPVCVHLHD
                     TRGMGIANASAALDAGARILDGSLGGLGGCPFAPGATGNVVFEDLVFLCESKGFDTGI
                     DIEKLVAVRSILRSEMPHEALYGGLARAGLPDRKSSVAA"
     gene            complement(3109..4305)
                     /locus_tag="VGA15_25265"
     CDS             complement(3109..4305)
                     /locus_tag="VGA15_25265"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9591035.1"
                     /translation="MTGTGLPLAGVRVVEMTHMVMGPTCGMILAQLGAEVIKVEPPAG
                     DKTRSLGGMGTSFFPLFNRGKRSVVLDFAKAEDRETMDRLLASADVFLENFRDGQLEK
                     QGLGADELRRNYPHLIVAGHKGFLSGPYEHRPALDEVVQMMSGLAAMTGTSAKPQRVG
                     SSANDIMGGMFGVISILAALYQKRSGAPGADIRIGLFENCLFLVAQHMVEYEMTGNRP
                     RSMPEREHAWPIYDIFETAGGERIFIGVVTEGHWQSFCREFGLQEFLDDPVLRTTTDR
                     ILARSRIIPRVTEVIRHCNAAELSAKLDRLNICFSPINRPEDLFDDPHVLRPGGLVNN
                     VNADGAPFRVPALPIEWNGASIGEGLKVPALGADTEAVRAELEGQEFLEGNGFSPTGK
                     TAVRLA"
     gene            4574..>5638
                     /locus_tag="VGA15_25270"
     CDS             4574..>5638
                     /locus_tag="VGA15_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9591036.1"
                     /translation="MSVAEMAAGTAHSAARGGPDEISRRLESLPASSYVWRLVILLSL
                     GGWFEIYDLFFTGYIAPGLTRSGLLTTTTQAFFGFSGIGAFVAATFAGLFVGTFFLGF
                     LADRFGRRSIFTWALLAYTAASVAMACQTTSGGMLLWRFIAGIGIGVEIITIDAYITE
                     LVPSWMRGRAFAINQAVMFTAVPVVAALAWWLVPLSPYEIDGWRWVVLTGAAGSMVIW
                     ILRLYVPESPLWLARHGRTEEAFEILRKLEASAGSSGARPQNTADAPPSRTVAKIGFA
                     ELFKSPYLSLVVLFMVFNFCQAFGFYGFANWVPTLLVEKGITVTKSLQYSFIVAFAYP
                     IAPLLAASFADRLERKWIICG"
BASE COUNT          979 a         1740 c         1795 g         1124 t
ORIGIN      
        1 ggttgccggc tggtttgtgc cgatggttat tgtcgtagcg ctggtcgcct ttggcgcctg
       61 ggctctgttt ggacccgaac ccaaaatggc gttcggcctg gtcgcagccg tcagcgttct
      121 gatcatcgcc tgtccctgcg cgctcgggct ggccaccccg atgtcgatca tggttggcgt
      181 ggggcgcggc gcgcaggccg gcgtcttgat caagaatgcc gaagccctgg agcgcatgga
      241 aaagatcgat acgctggtgg tcgacaagac ggggacactg accgagggca agccgaaggt
      301 cgtctcgatc attgctacgg cgagatttca agagagcgag attctgcggc tcgccgcgag
      361 cgtagagcgc gccagcgagc atccgctcgc cgatgcgatc gtccgcgccg ccaaggagca
      421 caatctcgaa ttgagcaagg tagacgagtt cttttcgccg accggcaaag gcgcgaccgg
      481 caaggttgac ggcaagacta ttctcctggg caattccaac ttcctgacat ctgtcggcat
      541 cgaaacccag tcgctgaacg aacaaggcga gcgtctgcgc ggcgacggcg ccaccgtcat
      601 caatatcgcg gtcgacggca agctggccgg attgttcgcc atcgccgatc cggtcaagcc
      661 gtcgacgctg gatgcgttga agacgctggc ggcggagggc atcaaggtca tcatgctgac
      721 cggcgataac cgcaccacgg caaacgccgt cgcgcgaaaa ctcgggatat cagatgtcga
      781 ggcagaagtc ctgcccgatc agaagagtgc cgtcgtcaca aaactgcaga aggccgggcg
      841 gatcgtggcg atggccggcg acggcgtcaa cgacgccccc gcgctggccg cggccgacgt
      901 cggcatcgcc atgggcaccg gcaccgacgt cgcgatggag agtgccggca ttaccctgct
      961 gaaaggggat ctcggcggca tcgttcgcgc gcgccggctc tcgcaggcga ccatgggcaa
     1021 catccggcag aatcttttct ttgcattcat ctacaatgcc gccggcatcc cgattgccgc
     1081 gggcatcctc tatccccatt tcggtatcct gctctcgccg atcattgccg ccgcggccat
     1141 ggcgctgtcg tcggtgagtg tggtcggcaa tgcgctgcga ttgcgcgtga cgcggctgtg
     1201 agtcagcctg tggggcgcat cggagatcgt gcgcttggaa tcactggagt aaggccatgg
     1261 caaatttcac tccggtttcg gcagcgatcg gcggcggcct gatcggcctg tccgcggtct
     1321 tgctgatgct cttgaccggc cgcattgccg gcatcagcgg catatttggc ggcctgctga
     1381 atttcaaaag cgacgacagg gattggcgca tcgtctttat cgcggggctc attctggcgc
     1441 cgctgattgc aggctggatc ggctatgcga tgccgacacc aaagctgccg gcaagctgga
     1501 ccgtcatcat cggagccggc ctgctggttg gtttcggcac aagactggga ggcggctgca
     1561 cctcgggcca cggcatctgc ggaatagcgc gcctgtccag ccgttccatt gcggcaaccg
     1621 tcatcttcat gctggccgcg atcgcgacgg ttgcggtcac ccatcacttg ctcggaggct
     1681 gaaccatgcg gatgatcgca tccttggtgt gcggattggt gttcggcgcg ggccttttga
     1741 tttccggaat ggtccagccg acaaaggtct tgggatttct tgatatcttc ggcgcctggg
     1801 atccaagcct cgccgtggtg atggccgcgg cactcgcagt gtcgacgccg ggcttcatgc
     1861 tggcgaaggg ccgttcgcgg ccgctgcttg cgaccaaaag cttttggccg accaagcgcg
     1921 atatcgaccg gccgcttgtc gttggggcgg cgctattcgg actgggttgg gggcttgtcg
     1981 gcttgtgccc gggaccggcg ttggaaaatc ttgtgacgct gtcgcccggc atcattgtct
     2041 tcgtcgccgc catggctggc ggcatggcgc tgcatgactt ctggcaaaac tcgcaaccga
     2101 agattcggaa tagagaagct ttggccaacg ccgacggatg acagcccgaa gtgacggcgc
     2161 gaggtcaggc cgccacgctg gatttccggt cgggcagccc ggcgcgggcg aggccgccat
     2221 agagcgcctc atggggcatt tccgatcgca ggatcgagcg caccgcgacc agtttttcga
     2281 tatcgatgcc ggtatcgaat cccttgcttt cgcacagaaa taccaggtcc tcgaacacga
     2341 cgttgccggt ggcgccgggc gcgaacggac agccgccgag gccgcccagc gagccgtcga
     2401 ggatgcgcgc gccggcatcg agcgctgccg acgcgttcgc gatgcccatg cctctggtat
     2461 cgtgcagatg cacgcagacc ggctttgcgc cggatatgtt cacgatcgcc ttggtcagtt
     2521 cgccgacctg cttcggaccg gcatagccga cggtgtcggc gacggcgacg aaatcgacgc
     2581 cggcctcgag acatctttcg gcgagtttta tcacttcttt cgggtctacc ggccccgcga
     2641 tcgaacaacc gagcgccatc gagatcgccg cattgacgat cggcttgtgc ggacttgagt
     2701 cgcgcaactc gcaaagccgc cggacattgg caatggcctc ctcgcgggac ctgttcgcat
     2761 tggcctggct gtgctcctcg gtggccgaga ccacggatgc aatttcggca acgcctgacg
     2821 ccagcgcttc gttgacgccg cgttcgttga gcgccagcgc gatgccatgc gcccccggca
     2881 gcgatgccac ggctttgacg acgtcgcgca cgtcggcgaa ctgcgggaag gttttcgcag
     2941 ggagaaacga gccgacctcg aaatgccgca cgccggcggc gtattcgtcc cggagccagc
     3001 gttgtttcgc cgccgtcgac ggaaaagtct tcaccagttg caggccatcg cgcaaaccga
     3061 cctcgcgcaa gctcactctg tcggccggat agacgtcttg aacgcgggtc atgcgagcct
     3121 cacagcggtc ttaccggttg gagaaaaacc gttgccttca agaaattctt ggccctcgag
     3181 ctcggcgcga accgcttcgg tatcggcgcc gagcgccggc accttcagtc cttcgccgat
     3241 gctggccccg ttccactcga tcggaagtgc cggaacgcgg aacggcgcgc catcggcatt
     3301 gacgttattg acaagcccgc cgggccgcaa cacgtgcgga tcgtcgaaca ggtcttcggg
     3361 gcgattgatc ggcgagaagc agatgttcag ccggtcgagt ttcgcggaca gctccgccgc
     3421 gttgcagtgt cttatcactt cggtcacgcg cggaatgatt cttgatcgcg ccaggatccg
     3481 gtccgtggtg gtgcgcagaa cgggatcgtc cagaaactcc tgcaagccga actcgcggca
     3541 aaaactctgc cagtggcctt cggtgacaac gccgatgaaa atgcgctcgc cgccggcggt
     3601 ctcgaaaata tcatagatcg gccacgcatg ttcgcgctcc ggcatcgagc gcggtctgtt
     3661 gccggtcatc tcgtactcga ccatgtgctg ggcgaccagg aacaggcaat tttcgaataa
     3721 tccgatgcgg atatcggcac ccggagcgcc gctgcgtttt tgatagagcg cggcgagaat
     3781 ggagatcacc ccgaacatgc cgcccatgat gtcgttcgcc gacgatccga cacgctgcgg
     3841 tttagcgctg gtcccggtca tggcagcgag gccggacatc atctgcacca cctcgtccag
     3901 cgccgggcga tgctcgtagg gaccggacag aaagcctttg tgtccggcga cgatcagatg
     3961 cggatagttt cgccgtagtt catcggcgcc aagcccctgc ttttcaagtt gcccatcgcg
     4021 aaagttttcc agaaacacgt cggcgctggc caagagccgg tccatggtct cgcggtcctc
     4081 ggcctttgca aagtcgagca cgacgctgcg cttgccgcga ttgaacagcg ggaagaacga
     4141 cgtgcccatg ccgccgaggc tgcgggtctt gtcgccggcc ggcggctcga ccttgatgac
     4201 ctcggcgccg agctgcgcca gaatcatgcc gcaggtcggg cccatcacca tatgggtcat
     4261 ttcgacgacc ctcacgcccg ccagcggcaa tcccgttccc gtcatcatcg tctccgtgct
     4321 ttttcggtca tgctcgtcgt catcggccgc gcttcagact aggctttcag gcgcggtctg
     4381 gaaaatataa tctgggaaac ggtgtattca tcattccata acgcagctcc tggcggccaa
     4441 ctgccgattg agtgaaatat ccaacactgg aacgctttca aacgcagaat gaccggaaaa
     4501 gacattgacc ggaaaagaca tgaccggaaa agacttgccg caccaagcgg ccggccggct
     4561 tagggaggac catatgagcg tagccgagat ggccgctgga accgcgcata gcgccgcgcg
     4621 cggcgggccg gatgagatat cgcggcggct cgaaagcctg ccagcctctt cttatgtgtg
     4681 gcgtcttgtg atcctgctgt cgctcggcgg ctggttcgag atctatgacc tgttctttac
     4741 cggctacatc gcgccgggcc ttacccgcag cggattgctg accaccacca cccaggcctt
     4801 ttttggcttt tcggggatcg gcgcgtttgt ggcggcgacc tttgccgggc tgttcgtcgg
     4861 caccttcttt ctgggctttc tggccgaccg gttcggacgg cgctcgatct tcacctgggc
     4921 gctgctcgct tacaccgcgg cgtcggtggc gatggcatgt cagaccacat ccggcggcat
     4981 gctgctgtgg cggttcattg ccgggatcgg catcggcgtc gagatcatca ccatcgacgc
     5041 ctatatcacc gagctggtgc cgagctggat gcgcggccgc gccttcgcga tcaatcaggc
     5101 ggtgatgttc accgcggtcc ccgtggtggc ggcgctggcg tggtggctgg tgccgctgtc
     5161 gccgtatgag atcgatggct ggcgctgggt ggtgctgacg ggcgctgccg gcagcatggt
     5221 aatctggatc ttgcggctat acgtgccgga aagtccgctc tggctggcgc gccatggccg
     5281 aaccgaggag gccttcgaga tcctgcggaa actcgaagcc tccgcgggct ccagcggcgc
     5341 gcgtccgcaa aacacggcgg atgcgccgcc ctcgcgcacg gtggcgaaaa taggttttgc
     5401 ggagctgttc aagtcgcctt atttgtcact cgtcgtgctg ttcatggtgt tcaatttctg
     5461 ccaggccttc ggcttctatg gttttgccaa ctgggtgccg acgctgctgg tcgaaaaggg
     5521 catcaccgtc accaagagcc tgcaatattc cttcatcgtc gcgttcgcct acccgattgc
     5581 gccgctgctg gccgcgagct ttgccgaccg gctcgagcgc aaatggatca tctgcggc
//
LOCUS       DASWGM010000935         3558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1211077,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000935 DASWGM010000000
VERSION     DASWGM010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211077"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="VGA15_25275"
     CDS             <1..1667
                     /locus_tag="VGA15_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX9591037.1"
                     /translation="RARRRLQTGTLRGLALTDASANTSRKWRPSLSLVVFLVLTSVLS
                     LPLFSLYFLKVYQNELIQQTEAELIAQSAALSAVFHREIETSIPQAVALGTKVSPIAR
                     KPSEEPYQPIWPALELANESVLPPRPGARTPPTPADPAFVALGARMMPDLVATQNVTL
                     AGFRLLDPNGVVIAGHEEVGMSLAHLEEVSEALQGRFSGVLRVRISKHDQPALYSISR
                     GTGMRIFTAMPVIVRDQVAGVVYASRTPSNVFKYLYERRRRLMLAMLSMIVPTLLIGF
                     LFHRTITEPMRELVERTNLVGKGDRDALRPLKRHGTSEFARLSQSFLEMARRLNARSS
                     FISTFATHVSHELKSPLTSIQGAAELLRDDIDAATTPLMADRDKRKFLDNIIADTDRL
                     AKISGRLRDFARAENPVAVGAAKLSSLMAGLRSAFASLDIRTSGDLDTPMRISEENAG
                     IIFSNLADNAVRHGGATLDISATRQGNLLQVMVIDNGEGVSPNNRTQIFDSFFTTRRD
                     SGGTGMGLAIVRAMLDAHGGAVRLSDCEWGTAFELTIPIADEALVS"
     gene            1715..2137
                     /locus_tag="VGA15_25280"
     CDS             1715..2137
                     /locus_tag="VGA15_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HEX9591038.1"
                     /translation="MIMRAGLCLLIIVSGLALRKFGAGLGLPFFFVKYGGSVLWGTMV
                     FFLVAMAAPARSPSGIAMISASIAICVELFRLVHAPWLDDFRLTTLGALLIGRVFSPW
                     NMLAYGVGIGLGMWLDRLGMSVLTRARPSARNTPHSAS"
     gene            complement(2122..3285)
                     /locus_tag="VGA15_25285"
     CDS             complement(2122..3285)
                     /locus_tag="VGA15_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX9591039.1"
                     /translation="MPRAVQCHPLTKTQARRIWLRAQRLEKSAPFGEGPQATAAAVEH
                     LGYVQIDTINVIERSHHHILWTRIPNYRRADLRQAQSIDRSVFEYWTHALSYVPAKDL
                     RFFLPAMKRHKREGHKWLSSVKPADLRKVLRLIRRDGALTIRDIDDDVLVDKEHLWAS
                     RKPSKRALQLAFYTGVLTISERNGMLKTYDLMTRHFGWDRPPKPASAGETTAYLLDRA
                     LRAQGVVSLDSVCHLDAPSKPSIRRLIQTRVRRNELVPVALEGAGKQEHWARPETLEI
                     SDGGAFELVHILSPFDPLIIQRKRTELFFGYGHRFEAYVPREKRVFGYFALPVLVGEN
                     IVAAIDLKTDRKTRKLLMQKWNWVDAAPRGARKDLKRRIEEELHRFERFQLAE"
     gene            3315..>3558
                     /locus_tag="VGA15_25290"
     CDS             3315..>3558
                     /locus_tag="VGA15_25290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591040.1"
                     /translation="MKTSGFRKNACVVPSQGTKKAAPPEGRAATSLLLAIEIRFGFGR
                     VSSTKTILCAAMQWKRLGQAVFSPRPPSMNREPGVAQ"
BASE COUNT          650 a         1122 c         1080 g          706 t
ORIGIN      
        1 accgtgcacg gcgtcggctt cagactggga cgctgcgagg cctcgcgttg accgacgcct
       61 cggccaacac cagccgcaaa tggcggccga gtctcagcct cgtcgtcttt ctggttctga
      121 ccagcgtgct ttcgctgccg ctgttcagcc tgtattttct aaaagtctac cagaacgagc
      181 tgatccagca gaccgaggcc gaactgatcg cgcagagcgc ggcgctgtcg gcggttttcc
      241 atcgcgaaat cgaaaccagt attccccaag ctgtcgcgct cggcaccaag gtttcgccga
      301 tcgctcgaaa gccgtccgag gaaccctatc agccaatctg gccggcgctg gagcttgcca
      361 acgaaagcgt gctgccgcca aggccgggag ctcgcacacc gcccacgccg gccgatcccg
      421 ccttcgtcgc cctcggtgcg cgcatgatgc ccgatctcgt cgcaacgcaa aatgtgacgc
      481 tcgccgggtt tcggctgctc gacccgaacg gcgtcgtcat cgcggggcac gaggaagtcg
      541 ggatgtcgct cgcgcatctc gaggaagtca gcgaggcgct gcaagggcgc ttcagcggcg
      601 tcttgcgtgt gcgcatctca aagcatgacc aaccggcgct ctattcgatc agccggggca
      661 ccggcatgcg catctttacc gcaatgcccg tgatcgtacg cgatcaggtc gcaggcgtcg
      721 tttatgcctc gcgcacgccc agcaacgtct tcaaatacct gtatgagcgg cgcagacgac
      781 tcatgctcgc gatgctttcc atgatcgttc cgaccctgct gatcggattc ttgtttcatc
      841 gcaccatcac cgaaccgatg cgggagctgg tcgaacgaac caacctggtc ggcaagggcg
      901 accgcgatgc gctgcgcccg ctcaagcgcc atggcaccag cgagttcgcc cgcctgtcgc
      961 agagttttct ggaaatggca agaaggctga atgcgcgctc cagtttcatc tcgaccttcg
     1021 ccacccatgt atcgcatgaa ctgaaatcgc cgctgacctc gattcagggg gccgcggagc
     1081 ttttgcgcga cgatatcgac gcggcgacga cgcccttgat ggccgacagg gacaagcgaa
     1141 aatttctcga caacatcatt gccgacaccg acaggctggc gaaaatctcg ggccgcctgc
     1201 gcgatttcgc acgcgcggaa aatccggttg cggtcggcgc cgcgaaactg tcgagcctta
     1261 tggccggctt gcgttcggcg ttcgcgtcgc tcgacatacg cacgagcggc gacctcgata
     1321 cgccgatgcg gatatcggag gagaacgccg gcatcatttt ttccaatctc gccgacaacg
     1381 ccgtgcgcca tggcggcgcg acgctcgata tatccgcgac gcggcaagga aatctgctgc
     1441 aggttatggt catcgacaac ggcgaaggcg tctcgcccaa caaccggacg cagatattcg
     1501 acagcttttt taccacccgc cgcgacagcg gcggcaccgg catggggctt gccatcgttc
     1561 gcgccatgct cgacgcccat ggtggcgcgg taaggcttag cgactgcgaa tgggggacgg
     1621 cgttcgaact gacaattccg atagcggatg aggcactggt ttcatgaaca cagcgtcaaa
     1681 ccacttgacg cgagagcgca cttttggcgc tccaatgatc atgcgcgccg gcctgtgcct
     1741 gctgatcatc gtctccggtc tcgccttgcg caagttcgga gcgggccttg ggctaccctt
     1801 ctttttcgta aaatatggcg ggtccgtgct gtggggaacg atggttttct ttctggtagc
     1861 gatggccgct cccgcccgct caccgtcggg catcgcaatg atctcagcct cgatcgccat
     1921 ctgcgtcgaa ctgtttcggc ttgtccatgc cccatggctc gacgatttcc ggctgacgac
     1981 tttaggcgcg ctgttgatcg gccgcgtgtt ctcgccatgg aacatgctcg cctatggcgt
     2041 cggaatcggt ttgggaatgt ggctcgatcg cctggggatg tcggtcttga caagagcaag
     2101 accctcggca cgaaacaccc ctcactccgc gagctgaaac cgctcgaacc ggtgcagctc
     2161 ttcctcgatg cgacgcttta gatccttgcg cgcgccccgc ggcgcggcgt cgacccagtt
     2221 ccatttttgc atcagcaatt tgcgggtctt gcggtcggtc ttgagatcga tggcggcgac
     2281 gatgttttca cccactagaa ccggcaacgc gaaatagccg aacacccgtt tttcccgggg
     2341 cacataggct tcgaagcgat ggccgtagcc gaaaaacagt tcggtgcgct tgcgctgaat
     2401 gatgagcgga tcgaacggcg agaggatgtg caccagttcg aaagcgccgc cgtccgagat
     2461 ctccagcgtt tcgggccgcg cccaatgttc ctgcttgccc gcgccctcca gcgcgaccgg
     2521 caccagctcg ttgcgacgga cccttgtctg gatcaaccgc cggatcgagg gcttgctcgg
     2581 cgcatcgaga tggcagaccg aatccagact cacgacgcct tgcgcgcgca acgcgcggtc
     2641 gagcagatag gcggtggttt cgcccgccga agccggtttt ggcggcctgt cccagccaaa
     2701 gtgccgcgtc atcaggtcgt aagtcttgag catgccgttg cgctcgctga tcgtcagcac
     2761 gccggtatag aacgccagtt gcagcgcccg cttcgacggc ttgcggctgg cccacagatg
     2821 ctccttgtca accagcacat cgtcgtcaat gtcgcggatg gtcagcgcgc cgtcgcgccg
     2881 gatcaagcgc agcaccttgc gcaagtcggc cggcttgacc gagctgagcc atttatggcc
     2941 ctcgcgcttg tgacgtttca tggcgggaag gaagaagcgc agatcctttg ccggcacgta
     3001 ggacagcgca tgggtccagt attcgaacac gcttctgtcg atgctctggg cctggcgcag
     3061 atccgcgcgc cggtaattcg ggatgcgggt ccacagaatg tgatggtggc tgcgctcgat
     3121 gacgttgatg gtgtcgatct ggacatagcc gagatgctcc accgccgcgg ccgttgcctg
     3181 cggcccttca ccgaacggcg ctgacttctc gagtcgctgg gcgcgcagcc agatccgccg
     3241 ggcttgcgtc ttggtcaggg ggtggcattg aacggcgcgg ggcatggcgt cgcaatgtag
     3301 ccggattcgc ggctgtgaag accagcggat tccgcaaaaa tgcgtgcgtc gtcccttcgc
     3361 aaggcacgaa aaaagccgcg ccgcccgagg gccgcgcagc taccagcctg ttgctggcta
     3421 tcgagatacg ttttggcttc gggcgcgttt cttcaaccaa gacaattctg tgtgctgcga
     3481 tgcagtggaa aagactcggt caggccgttt tttcgccaag accaccgtca atgaaccggg
     3541 aacccggcgt cgcacaat
//
LOCUS       DASWGM010000936         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_655523,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000936 DASWGM010000000
VERSION     DASWGM010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655523"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..338)
                     /locus_tag="VGA15_25295"
     CDS             complement(138..338)
                     /locus_tag="VGA15_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX9591041.1"
                     /translation="MRSLAFADFLIGLGILFVLEGLLFAASPAWMRRAMKSALATPDN
                     ILRAVGIGSAVAGLILIWLVRH"
     gene            complement(451..1377)
                     /gene="hflC"
                     /locus_tag="VGA15_25300"
     CDS             complement(451..1377)
                     /gene="hflC"
                     /locus_tag="VGA15_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX9591042.1"
                     /translation="MRSPVAGIAALFVLFVVLIVGYSSVFTVAQTEQVLVVRLGEPVK
                     VVTEPGLNFKAPFIDTVIAIDKRILDLENPSQEVIASDQKRLVVDAFARYRIKNALRF
                     YQSVGSIQAANIQLTTLLNASLRRVLGEVTFITVVRDQREALMARIRDQLDKEADGYG
                     IQVVDVRIRRADLPEQNSQAVYQRMQTERQREAAEFRAQGGQKAQEIRSNADREATVI
                     VAEANSTAEQVRGEGDGERNRLFADAYGKDPDFFAFYRSMTAYENGLKSNDTRFLLRP
                     DSDFFKFFSNSSGKPPAAPALPAATTAAAPKP"
     gene            complement(1374..2528)
                     /gene="hflK"
                     /locus_tag="VGA15_25305"
     CDS             complement(1374..2528)
                     /gene="hflK"
                     /locus_tag="VGA15_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX9591043.1"
                     /translation="MPWKNQGGGPWGSGPKGPWGSGPQSVGPRPPDLEDLLRRGQDRL
                     QQLLPGGYFSGIGVALLLVGALAIWGMSGFYRVQSEELGVVLRFGKYVGDAQPGLNYH
                     LPYPIETVLLPKALRVSTLSIGMTLIDDPARRGRTMRDVPEESLMLTGDENIVDVDFT
                     VLWRIKPKGVADFLFNIQNPEGTVKAVAESAMREVVGRSNIQPILTGARTTNEAAVQE
                     LMQKTLDSYGSGILVQQVQMQKVDPPAQVIDSFRDVQAARADLERLQNEAQTYANRVV
                     PDARGRAAQILQVAEGYKQQAVAEAKGQSARFLKVYDEYKKAPDVTRQRIYLETMERI
                     LGGAEKLVYDSGGSSSAQSVVPYLPLNELTPRRPPAATTGQPQQPSGATR"
BASE COUNT          445 a          838 c          780 g          488 t
ORIGIN      
        1 tgagcgtcac gtcccaaact cgatcgccac cgtgcgccga cgcatcatcg acgctctcgt
       61 caggcgcttg ccacgatgcc catgctgtcg cgcttcaacc gcgcgacgcg tgcggatgaa
      121 cttgtgacgc agtagtacta gtgacggacc agccatatca aaatcagacc cgcgaccgcc
      181 gagccgatgc cgacggcgcg cagaatgttg tccggcgtcg ccagcgcgct cttcatggcc
      241 cggcgcatcc aggccgggct tgccgcaaac aacaaccctt ccagcacaaa cagaatgccc
      301 aaaccgatga ggaagtcggc gaacgctaag gacctcatcg gactgaaacc tcccgcttga
      361 tgttttttct tatgccattt ggggcgaagc gaagttcgct tcgacccggt cttgtttgtt
      421 gtttagccga gctgccctgg gaaggcggtc ttacggcttc ggcgccgccg cagtggtcgc
      481 tgccggtagc gcaggcgccg cgggcggctt gcccgacgag ttgctgaaga acttgaagaa
      541 gtcggaatcc ggccgcagca ggaagcgggt atcgttgctc ttcaagccgt tctcgtacgc
      601 cgtcatcgag cggtagaatg cgaagaaatc aggatccttg ccgtaggcgt cagcgaacag
      661 ccggttgcgc tcgccatcgc cctcgccgcg cacctgctcg gcggtggaat tggcttcggc
      721 gacgatgacg gtggcttcac gatccgcgtt ggacctgatc tcctgggcct tctggccgcc
      781 ctgggcgcgg aattcggcgg cctcgcgctg ccgctcggtc tgcatgcgct ggtagaccgc
      841 ctggctgttc tgctccggca gatcggcacg cctgatccgc acatcgacca cctggatgcc
      901 atagccatcg gcttccttgt cgagctgatc acggattcgc gccatcaggg cttcgcgctg
      961 gtcccgcacc accgtgatga aggtcacctc gccgagcacc cggcggagcg aggcgttcaa
     1021 gagcgtcgtc aactggatgt tggcggcctg gatcgagccg acgctctggt aaaatcgcag
     1081 cgcgttcttg atgcgatagc gcgcgaaggc gtcgaccacc agccgcttct ggtccgacgc
     1141 gatcacttcc tgcgacggat tttcgaggtc gaggatgcgc ttgtcgatgg cgatcacggt
     1201 gtcgatgaac ggcgccttga aattcagccc cggctcggta accaccttga ccggctcacc
     1261 gagccgcacc accagcacct gttcggtctg cgccacggta aagaccgagc tgtagccgac
     1321 gatcagaacg acaaacagca cgaacagcgc ggcaatacct gcaactgggg atctcatcgc
     1381 gtggctccgc tcggctgttg cggctggccc gttgtcgccg caggcgggcg ccgcggcgtg
     1441 agctcattca gaggcaaata gggcacgaca ctctgcgctg atgacgaacc gccgctgtca
     1501 tagaccagct tctcggcacc gccgagaatg cgctccatgg tttccagata gatgcgctgc
     1561 ctcgtcacgt caggcgcctt cttgtattcg tcgtagacct tcaggaaacg cgcgctctgg
     1621 cccttggcct cggcgaccgc ctgctgcttg tagccctctg cgacctgcag gatttgcgcg
     1681 gcgcgtccgc gcgcgtccgg cacgacgcga ttggcatagg tctgcgcctc gttctgcaag
     1741 cgttcgagat cggcgcgcgc cgcctgcacg tcgcggaacg aatcgatcac ctgcgccggc
     1801 ggatcgactt tctgcatctg cacctgctgc accagaatgc cggaaccgta gctgtcgagc
     1861 gtcttctgca tcagctcctg cacggcggcc tcgttggtgg tgcgagcgcc ggtgaggatc
     1921 ggctggatgt tggagcggcc gacgacttcg cgcatcgcgc tttcggcgac cgctttcacg
     1981 gtgccttcgg gattctggat gttgaagaga aaatcggcga cgcctttcgg cttgatccgc
     2041 cacagcacgg tgaagtcgac gtcgacgata ttttcgtcgc cggtcagcat caggctttct
     2101 tccggcacgt cgcgcatcgt cctgccgcgc cgcgccgggt cgtcgatcag cgtcatgccg
     2161 atgctcagcg tcgaaacccg cagcgctttc ggcagcaata cggtctcgat cggataggga
     2221 agatggtagt tcagtccggg ctgggcgtcg cccacatact tgccgaagcg aagcacgacg
     2281 ccgagttctt cggattgaac ccgataaaat ccggacatgc cccagatcgc cagcgcgccg
     2341 accagaagca gcgcgacgcc gatgccgctg aaatacccgc ccggcaacag ctgctgaagc
     2401 cggtcctgac cacggcgcag aaggtcttca agatcgggcg gcctcggccc caccgattgc
     2461 gggccggagc cccacggccc ttttggaccc gagccccatg ggcctccgcc ttgattcttc
     2521 cacggcatcg acagtctcct ctgcgggctc a
//
LOCUS       DASWGM010000937         5515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1618286,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000937 DASWGM010000000
VERSION     DASWGM010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618286"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VGA15_25310"
     CDS             <1..177
                     /locus_tag="VGA15_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX9591044.1"
                     /translation="HALCSAIPELRRVHYVQKGVGHYGVFNGSRFKSEIVPRISDFMV
                     SAANAKPSLRAAAE"
     gene            407..1246
                     /locus_tag="VGA15_25315"
     CDS             407..1246
                     /locus_tag="VGA15_25315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX9591045.1"
                     /translation="MICFCAERFHWRQIWQNPGELLTSGLTDMATRALLYRRPAEPSR
                     LLVKHGSQIYSIRLRRHRRARRYTLRIHPSDREAILTMPPRGTIIDAKDFAQRHGGWI
                     AARLGRLPKAAPFHSGTVVPLRGLPYRIVHRAGLRGTVWTEIRDSGERILCVAGGVEH
                     IDRRVHDFLKREARKDLQKAAQSYAQALGVKVKRLSIRDQSSRWGSCTSAGSLSFSWR
                     LILAPPYVLDYLAAHEVAHLVEMNHSARFWRVVAKVCGHVERAKTWLDAHGNDLHRYG
                     IED"
     gene            complement(1487..2740)
                     /locus_tag="VGA15_25320"
     CDS             complement(1487..2740)
                     /locus_tag="VGA15_25320"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX9591046.1"
                     /translation="MNKRSVFGILSITTLGLALLVGPVRADPISDWNAKAQAIQIEKQ
                     LPPAVSAREMAILHVAMFEAVNAIDRRYAAYRLNLPAEPGFSKEATAAAAAYAVLIAL
                     YPDQQPKLDAALASSLAGIAEGEPKAKGIDLGKKAAAGILALRANDGANAPETYRPHT
                     SAGVYVPTVVPVFSTVGTITPWVMTAGSQFRPPPPPALTSPTWTKDVNEIRELGSRTS
                     TVRTAEQTNIGRFWFFVGPQTWTPILRQVAASKDMELVDCARLFALASMAGHDAGIAV
                     FDAKYTYNLWRPITAIRNADLSKNDATPREASWLPLGDTPMHPEYPCAHCITSAAVGT
                     IIQKVVGNDVAEITLTSPTAPGVTRKWTRIQDYMDEVSQARIYAGFHYRFSTVTGADM
                     GRKIAELAVTTKLLGAKAPRANLGD"
     gene            complement(3152..5368)
                     /locus_tag="VGA15_25325"
     CDS             complement(3152..5368)
                     /locus_tag="VGA15_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX9591047.1"
                     /translation="MAWGRKKGGGRKEPQFGLAASLAELRLSPQDRVTVGDEDDEDET
                     PKKPSAKRKARDADDDAPRERKPRPSPVSRTRKSKTRSPVGISRLFYWAAVLGLWAAI
                     AVVGVIVWVGAHLPAIQSLEIPNRPPTIQITGFDGSVLATRGEMAGANVALKDLPPYL
                     PKAFIAIEDRRFYAHYGVDPVGIARAAVANILHRGVSQGGSTLTQQLAKNLFLTQERT
                     LQRKLQEVELAFWLERKHSKSEILELYLNRVYFGSGAYGVEAAAQRYFGKSAKNVTIA
                     EAAMLAGLVKSPTRLAPNRNPEGAEKRAQTVLSAMADAKFITEAQAQASIGHPSYHVK
                     AAGAGTVNYVADWIGEVLDDLIGPVEQSIVVETSIDPKLQAVAEASVIDELAAKSVKF
                     NVSQGALVAMTPDGAVRAMVGGRNYAESQYNRAVTAKRQPGSAFKPFVYLTAIEAGLT
                     PETIRQDAPLDVKGWKPENYSHEYLGAVTLTQALAMSLNTVAVRLGLEVGPKNVVRTA
                     HRLGIASKLETNASIALGTSEVSLTELVGAYAPFANGGQGVTPHVVTKIRTSEGKVLY
                     ARLPDQLGQVIEPRNVAMMNTMMQETLLSGTARKAEIPGWVAAGKTGTSQDFRDAWFV
                     GYTANLVTGVWLGNDDNSPTKKATGGGLPVEVWTRFMRAAHQGVPIAALPNSQAGGFF
                     SNLAQTASQVSAPSPPTATAPQAASGAARPAPTRTSVRPDAATGLDGWLMDRLFGR"
BASE COUNT          971 a         1735 c         1729 g         1080 t
ORIGIN      
        1 cacgcgctgt gcagtgctat tcccgaactt cgccgggtgc attacgtgca aaaaggtgtt
       61 ggacattacg gtgtgttcaa cggatcccgc ttcaaatcgg aaatcgtgcc tcgcatctcc
      121 gatttcatgg tgtcagcggc gaacgcgaag ccttcgttac gcgcggcggc ggaataaacg
      181 ccgatcaaat gcatcgacgt ttcgcgattg aaacgtcgat gctgattcga tcggcccggt
      241 aaccctctga ataggcccat acaaacccgg attcacgaat ctctgaatga atgagtctta
      301 cctcagttct tcggaggctc gtttggggct cttctcagcc aatattcccc gttccgggcc
      361 cattctgtag gggcgactcg gcggtcagcc cctgtatatt gggcaaatga tttgtttttg
      421 cgccgagcga tttcactggc ggcagatatg gcagaatccg ggcgaactcc tcacgtccgg
      481 actgacagac atggccactc gcgccctcct ttatcggcgg cctgccgaac cttctcgcct
      541 tttggtcaag catggctccc aaatctactc gatcaggctg cgccggcacc gccgcgcgcg
      601 gcgctacacc ttgcgcattc atccaagcga ccgcgaagcc atcctgacca tgccgccgcg
      661 cggcactatt atcgacgcca aggatttcgc gcagcgtcac ggcggctgga tcgccgcgcg
      721 cctcggccgg ttgccgaagg ccgcgccgtt tcattcgggc accgtggtgc cgttgcgtgg
      781 gctgccgtat cggatcgtgc atcgggcggg cttgcgcggc acggtgtgga cggaaatccg
      841 cgacagcggc gagcggattc tctgcgtcgc agggggcgtt gagcatatcg accgccgggt
      901 ccacgacttc ctcaagcgcg aagcgcgcaa ggacctgcag aaggcggcgc agtcctatgc
      961 ccaggcactc ggcgtcaagg tcaagcggct gtcgatccgc gatcagtcga gccgttgggg
     1021 ttcgtgtact tcggctggct cgctgtcgtt ctcctggcgg ctgatcctcg cgccgcccta
     1081 tgtgctcgat tatctcgccg cccacgaggt cgcgcatctc gtcgagatga accattcggc
     1141 gcggttctgg cgggtggtcg cgaaagtctg cggtcatgtc gagcgcgcca agacctggct
     1201 cgatgcgcac ggcaacgacc tgcatcgcta cggcatcgag gattaagctg gcctcgtgtc
     1261 ccggacgcgg cgcggcacga agtgacgcga ggcagagccg ggacccacca cgccgccgcg
     1321 ttcgcacaac gccgcttctc taccgtccga acagccggtc catcagccag ccgtcgagtc
     1381 cggccgctgc ttcgggcgaa gttggcttgg agtggcgagg gcgcaaggaa cagacagcaa
     1441 gaagccccgc ccggcttgag gccgcgtggg gccgtcttgt cggggctcag tctcccaaat
     1501 tggcgcgggg ggccttcgcg ccaagcaact tggtggtcac cgccaattcc gcgatcttcc
     1561 gtcccatgtc cgcgccggtc acagtcgaga agcgataatg gaagcccgca taaatcctgg
     1621 cctgcgaaac ctcgtccata tagtcctgga ttcgcgtcca cttgcgggtg acgccaggag
     1681 cggtcgggct cgtcagcgtg atctcggcga catcgttacc caccaccttc tggataatcg
     1741 tgccgaccgc ggccgaggta atgcagtgcg cacacggata ttccgggtgc atgggggtat
     1801 cacccagcgg cagccaggat gcttcccgcg gcgtggcgtc gttcttgctc agatcggcat
     1861 tgcggatcgc ggtgatagga cgccagagat tgtaggtata ttttgcatcg aatacggcga
     1921 tgccggcgtc atggccggcc atggaggcaa gcgcgaacag acgtgcacag tcaaccagct
     1981 ccatgtcctt gctggccgca acctgtcgaa gaatgggagt ccatgtctgc gggccaacga
     2041 agaaccagaa gcggccaata ttggtctgct cggccgtacg cacggtgctg gtgcggcttc
     2101 ccagttcccg gatctcgttc acatcctttg tccaggtcgg cgaagtaagc gccggcggcg
     2161 gcggcgggcg gaattgcgag ccggccgtca tcacccaagg tgttatcgtg cccacagtgg
     2221 agaatacagg aacgaccgtc ggcacataga cgcccgcgct tgtatgggga cgataggttt
     2281 ccggagcgtt tgccccgtca ttggcccgca gcgcgagtat cccggccgcg gctttcttgc
     2341 cgagatcgat tccctttgct ttcggctcgc cttcagcgat gccggccagc gaagacgcca
     2401 aggcggcgtc gagcttcggt tgttggtcgg gatatagagc gatcaagacc gcataggccg
     2461 cggctgcggc cgttgcttcc ttcgagaagc ctggttcggc gggcaggttg agccgatatg
     2521 ccgcgtagcg tcgatcgatg gcgttcaccg cctcgaacat ggcaacgtgg agaatagcca
     2581 tctcgcgcgc gctgaccgct ggcggaagtt gcttttcaat ctggatggcc tgggcctttg
     2641 cgttccagtc ggaaatcgga tcggcgcgca cgggcccgac caaaagcgca agccctagcg
     2701 tcgtgatact gagaatgccg aaaacactcc tcttgttcat tgcttcctcc attgatggtc
     2761 gtcacacggc acagcctcag gcgtatccgc cgaagctgtg gtcgaaaatc atctatctgc
     2821 caatattcgg agtgctcgcc cgccgatggg catcatgcgc tgccattaaa gcggccttcg
     2881 gcacgacctg cagcagctcc aaaaaagtct acgtcaattg ccgcgttgcg atcttgtgtg
     2941 agccaattat cctggatcaa gtatgaagtg gttcacactt cacgactgct acaagtagtc
     3001 gggcatgaga cgatgccatg catcgattca tgcccagccg taacttctgc aataggcgct
     3061 tcccggacat ggcgcgatgt ccgacgtgag tccgctcttg ccctgtacca cccgatagca
     3121 gacgcaaggc gagaatgcgc gcgaacaaaa tctaccgtcc gaacagccgg tccatcagcc
     3181 agccgtccaa tcctgtcgcg gcgtccgggc gcaccgaggt tcgcgtcggc gccggtctgg
     3241 ccgcgccgct cgcggcctgc ggcgcggtgg ctgtgggcgg tgagggtgcg ctgacctgcg
     3301 aggcggtctg cgccaggttc gagaagaaac cacccgcttg cgagttcggc agcgccgcga
     3361 tcgggacgcc ctgatgagcc gcgcgcatga agcgggtcca gacctccacc ggcaacccgc
     3421 cgccggtggc ctttttggtc ggcgaattgt cgtcattgcc gagccagacg ccggtcacga
     3481 gattggcggt gtagccgacg aaccaggcgt cgcggaaatc ctggctggtg ccggtcttgc
     3541 cggcggccac ccagcccgga atttcggcct tgcgcgcggt gccgctcagc agggtctcct
     3601 gcatcatggt gttcatcatc gcgacattgc gcggctcgat cacctggccg agctgatccg
     3661 ggagacgggc gtacaacacc ttgccctcac tggtgcgaat cttggtcacg acatgcgggg
     3721 tgacgccctg gccgccattg gcgaacggcg cataggcgcc gaccagttca gtaagcgaga
     3781 cttcggacgt gcccaatgcg atcgacgcat tggtctcgag tttcgacgca atgccgagcc
     3841 ggtgcgcggt gcgaaccacg ttcttaggtc cgacttcgag ccccagccgc accgcgacgg
     3901 tattgagcga catcgccagc gcctgcgtca atgtcaccgc gccgagatac tcatggctgt
     3961 aattttcggg cttccagccc ttgacgtcga gcggcgcatc ctgccggatc gtctccggcg
     4021 tcagaccggc ttcgatcgcg gtgagataga cgaagggctt gaacgccgag cccggctggc
     4081 gttttgcggt gaccgcgcga ttatactggc tctcggcgta attgcggccg ccgaccatgg
     4141 cgcgaacggc gccgtccggc gtcatcgcca ccagtgcgcc ctggctgaca ttgaatttta
     4201 cgcttttcgc cgcgagctcg tcgatcacgg atgcttcggc aaccgcctgc agctttggat
     4261 cgatcgaggt ttcgaccacg atgctctgct cgaccgggcc gatcagatcg tcgagcactt
     4321 cgccgatcca gtccgcgaca tagttgacgg tgcccgcgcc cgccgctttc acatggtaag
     4381 agggatgtcc gatcgaggcc tgcgcctgcg cttcggtgat gaatttggcg tccgccatcg
     4441 cggacagcac ggtctgggcg cgtttctcgg cgccttccgg attgcggttc ggcgccagcc
     4501 gcgtcggcga tttgacgagc cccgcgagca tcgcggcctc cgcgatcgtg acgttctttg
     4561 cggatttccc gaaatagcgc tgcgcggccg cctcgacgcc ataggcgccg gaaccgaaat
     4621 acacccggtt gaggtagagt tcgagaatct ccgacttgga atgcttgcgc tccagccaga
     4681 acgcgagttc gacctcctgc aatttccgtt gcagggtgcg ctcctgcgtc agaaacagat
     4741 tcttggcgag ctgctgggtc agcgtcgagc cgccctgcga aaccccgcga tgcaggatat
     4801 tggcgaccgc cgcgcgcgcg atgccgaccg gatcgacgcc gtaatgcgca tagaaccggc
     4861 ggtcctcgat cgcgatgaag gctttcggca gatagggcgg cagatccttc agcgcgacat
     4921 tggcgccggc catttcgccg cgcgtcgcca gcacgctgcc gtcgaagcct gtgatctgga
     4981 tcgtcggcgg gcggttgggg atttcaagcg actggatcgc cggcagatgc gcgcccaccc
     5041 agacgatgac gccaaccacg gcgatagcgg cccacaggcc gagcaccgcg gcccaataga
     5101 acagccgcga aatgccgacg ggggagcgtg ttttggattt gcgcgtgcgg ctcaccggac
     5161 taggacgcgg ctttcgctcg cgcggagcgt cgtcatccgc gtcgcgggcc ttgcgtttgg
     5221 cagacggctt tttcggcgtc tcgtcttcgt cgtcctcgtc gccaacggtg acgcgatcct
     5281 gcggactgag acgcagttcg gcaagcgacg cggcgagccc gaattgcggc tctttgcgtc
     5341 cgccgccctt ttttcgtccc cacgccatag cgacaacgtc cgcacccgcc gatgcgagag
     5401 actagcgggc ggggtttaag gctgggttaa gcgatgatta atgctgatgc cgtgaggagc
     5461 ccgcaagtgc ctgagaaaca agcacttcag gactcttaac cataccgtca gcaat
//
LOCUS       DASWGM010000938         3745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1914902,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000938 DASWGM010000000
VERSION     DASWGM010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914902"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VGA15_25330"
     CDS             <1..814
                     /locus_tag="VGA15_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX9591048.1"
                     /translation="RLLLIGIPVFIWTMLPIYHMFLFAISPKEDAFAGKFWPDHPTLH
                     NFEIVFRQQHYFLRDFWIQFWNSTVIAVAVGALTLFIATAAAFSISRLRVLGGRWVMN
                     LALFTYFIPAAFLAVPMYRTMGTYGLLNNHWSLILAMVTIASPYAIWVLKQASDKLPV
                     ELDEAAIMDGATTLQLFRLVYVPLMMPSLVAIGTYALLLAWNEYLYAFLLLSKDTDIT
                     LPVALGNFLAADDSPWELLMTTGFIYALPPAAIYYAFKRYMVGGLTAGAVKS"
     gene            complement(816..1631)
                     /locus_tag="VGA15_25335"
     CDS             complement(816..1631)
                     /locus_tag="VGA15_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9591049.1"
                     /translation="MARAPNKISPFNVVSLALGLAFLYLPIVILVIYSFNASRLVTVW
                     GGWSVRWYAELFHDRAMLDAASMSFRVATVSATIATLLGTLAAVALSRGERFKGRTLF
                     SGMLYAPLVMPEVITGLSLLLLFVALNAERGFWTVTIAHTTLTMCFVAVVVQSRLGPL
                     DRSLEEAAMDLGCGPARAFLRVTLPLILPAIAAGWMLAFTLSLDDLVIASFTTGPGSA
                     TLPIRIYSEVRLGVKPEINAICTLIIGLIAMVIVVASFASKLSSARGDSAAPL"
     gene            complement(1631..2545)
                     /locus_tag="VGA15_25340"
     CDS             complement(1631..2545)
                     /locus_tag="VGA15_25340"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9591050.1"
                     /translation="MSVRRTFTRPARISAIAPYLWMALFFLVPFGFVLKISLSQTVIA
                     QPPYQPVFDLTQGWASIKAALAALSLDNFRLLVTDNLYVLSYLRSLFVAAVSTSILLV
                     IGYPIAYGMARLPRRWQGVAMMLVIVPFWTSFLIRIYAWINILQHDGLLNNVLIALHL
                     VSAPVVWLSTDSAMYLGIVYSYLPFMILPLYATLAKMEPAWLEAAGDLGASPWRTFWL
                     VTFPLSLPGVGAGVLLCFIPIVGEFVIPDLLAGSNSMMIGQTLWLEFFTNKDWPVASA
                     AAVVLLLLLLAPLWFYDRLQRRQFEDAR"
     gene            complement(2542..3690)
                     /locus_tag="VGA15_25345"
     CDS             complement(2542..3690)
                     /locus_tag="VGA15_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591051.1"
                     /translation="MADELPKPEPLGYTTDVPLLRIDAVVKKFGRFKAVDRLSLDIRA
                     GEFFALLGPSGCGKTTLLRMLAGFETPDEGRILLDGKDIAQVLPHRRPVNMMFQNYAL
                     FPHLTVRDNIAFGLKRAGMAHAEMDKRVAGLVALLKLQGLEKRRPDQLSGGQKQRVAL
                     ARALARRPQVLLLDEPLAALDKKLRESTQLELMELQRRLGMTFIIVTHDQEEAMTVAH
                     RIGVMDGGRIEQVATPRELYEAPTSRWIAEFVGDVNMFEGEVGKREAGRLTISTKDGG
                     AIVAAEPRQPITNTAICVAIRPEKVKLSRRGPVSDAGNAHAINRLEGIITEVNYLGGL
                     TTYKVKLDSGAVLRSSMANTARLEFDAYMASQRVVAWFTPDDCVVLER"
BASE COUNT          725 a         1236 c         1156 g          628 t
ORIGIN      
        1 gcggctgctg ctgatcggca ttcccgtgtt catctggacc atgctgccga tctatcacat
       61 gttcctgttc gcgatctcgc cgaaggaaga cgcgttcgcg ggcaaattct ggcccgatca
      121 tccgacgctg cacaatttcg aaatcgtgtt tcgccagcag cactattttc tgagggattt
      181 ctggattcag ttctggaatt cgacggtgat cgccgtcgcg gtcggcgcgc tgacgctctt
      241 catcgcgacc gcggctgcgt tctcgatctc gcggctgcgc gtcctcggcg gccgctgggt
      301 gatgaatctc gcgctgttca cctacttcat cccggcggcg ttcctcgccg ttccgatgta
      361 ccgcaccatg ggcacctatg gactcctcaa caatcactgg tcgttgattc tggcgatggt
      421 gaccatcgca tcgccttacg cgatctgggt gctcaagcag gcctccgaca agctgccggt
      481 ggagctcgac gaggccgcga tcatggacgg tgcgacgacc ttgcagctgt tccggctggt
      541 ctatgtgccg ctgatgatgc cgtcgctggt ggcgatcggc acctatgcgc tgctgctggc
      601 ctggaacgaa tatctctacg cgttcctgct cttgtcaaaa gacaccgaca tcaccctccc
      661 cgtcgcgctc ggcaacttcc tcgccgccga cgattcgccg tgggagctat tgatgaccac
      721 cggcttcatt tacgcgctgc cgccggccgc gatctactac gcgttcaagc gctacatggt
      781 gggcggcctg accgccggcg cggtgaaatc gtgacctaca acggcgcggc gctgtcgccg
      841 cgcgcgctcg acagtttcga ggcgaacgac gccaccacga tcaccatcgc gatcaatccg
      901 atgatcaacg tgcagatggc gttgatctcc ggcttcaccc cgagccgcac ctccgaatag
      961 atccggatcg gcagcgtcgc cgaaccagga cccgtggtga agctcgcgat caccagatcg
     1021 tccagcgaca gcgtgaacgc cagcatccag cccgcggcga tcgcgggcag gatcagcggc
     1081 agcgtgacgc ggagaaacgc ccgcgccgga ccgcaaccga gatccatcgc cgcctcctcc
     1141 aggctgcgat cgagcgggcc gagccgcgac tgcaccacca cggcgacgaa gcacatggtg
     1201 agcgtggtat gggcgatggt cacggtccag aagccgcgct cggcgttcag cgccacgaac
     1261 aacaatagca gcgaaagccc ggtgatgact tcgggcatca ccagcggcgc atagagcatg
     1321 ccggaaaaca gcgtgcggcc cttgaagcgc tcgccgcgcg acagcgccac cgcggcgagc
     1381 gtgccgagca gcgtcgcgat cgtggccgat accgtcgcca cgcggaaact catcgacgca
     1441 gcgtccagca tggcgcggtc gtggaacaat tcggcatacc agcgcaccga ccagccgccc
     1501 cacaccgtca ccagccgcga ggcgttgaac gaatagatca cgagaatgac gatcggcagg
     1561 tacagaaacg caaggcccag cgccagcgag acgacgttga acggcgagat cttgttcggc
     1621 gcgcgggcca ttatcgcgcg tcttcgaact ggcggcgctg cagccggtcg tagaaccaca
     1681 atggcgccag cagcagcagg agcagcacca ccgcggccgc cgaggccacc ggccagtcct
     1741 tgttggtgaa aaattccagc cacagcgtct ggccgatcat catcgaattg gaaccggcca
     1801 gcaggtccgg tataacaaac tccccgacga tcggaatgaa gcacagcaat acgccggcgc
     1861 cgacgcccgg caacgacagc ggaaacgtca ccagccagaa cgttcgccac ggcgaagcgc
     1921 cgagatcgcc tgctgcctcc agccacgccg gctccatttt ggcgagcgtc gcatagagcg
     1981 gcaggatcat gaacggcaga taggaataga cgatgccgag atacatcgcg ctgtcggtgg
     2041 acagccacac cactggcgcc gagaccagat gcagcgcgat caggacgttg ttgagcaggc
     2101 cgtcgtgctg caggatattg atccaggcat agatgcggat cagaaacgag gtccagaacg
     2161 gaacgatcac cagcatcatc gcgaccccct gccagcgccg cggtagccgc gccatgccgt
     2221 aggcgatggg gtagccgatc accagcagga tcgacgtcga gaccgcggcg acgaacaggc
     2281 tgcgcagata ggacagcacg taaaggttgt cggtgaccag cagcctgaaa ttatccagcg
     2341 acagcgccgc cagcgccgcc ttgatcgacg cccaaccctg cgtcaggtcg aaaacgggct
     2401 gatagggcgg ctgcgcgatg acggtctgcg acaggctgat cttcagcaca aaaccgaacg
     2461 gcaccaggaa gaacagcgcc atccacagat agggggcgat cgcgctgatg cgcgccgggc
     2521 gcgtgaaggt gcggcggacg ctcatcgctc cagcaccacg caatcgtcgg gggtgaacca
     2581 cgccaccacg cgctggcttg ccatgtaggc atcgaattcg agccgcgccg tgttggccat
     2641 cgacgaacgc agcacagcgc cgctgtcgag cttcaccttg taggttgtga gaccgccgag
     2701 atagttgacc tcggttatga tgccctccag ccggttgatg gcatgcgcat tgccggcgtc
     2761 ggacaccggg ccgcggcgcg acagcttgac cttctcgggg cggatggcga cgcagatcgc
     2821 cgtgttcgtg atcggctggc gcggctccgc ggcaacgatc gcgccgccgt cctttgtcga
     2881 aatcgtcagc cgacccgcct cgcgcttgcc gacttctccc tcgaacatat tgacgtcgcc
     2941 gacgaactcg gcaatccagc gcgaagtggg ggcctcgtaa agctcccgcg gcgttgcgac
     3001 ctgctcgata cggccgccat ccatcacgcc aatccggtgg gctaccgtca tcgcttcttc
     3061 ctggtcgtgg gtgacgatga tgaaggtcat gccgaggcgc cgctgcaact ccatcaattc
     3121 gagctgggtg ctttcgcgta gtttcttgtc gagcgcggcg agcggctcgt cgagcagcag
     3181 cacctgcggg cggcgcgcca atgcgcgcgc cagcgccacg cgctgctttt ggccgccgga
     3241 gagctgatcg ggccgccgct tctccagacc ctggagcttg agcagcgcga ccaacccggc
     3301 cacacgcttg tccatctcgg catgggccat cccggcgcgc ttcagcccga acgcgatgtt
     3361 gtcccgcacg gtgaggtgag gaaacagcgc atagttctgg aacatcatat tgaccggacg
     3421 ccggtgcggc agaacctggg cgatatcctt gccgtcaagc aggatccggc cttcgtccgg
     3481 cgtctcgaag ccggcgagca tccgcaacag cgtggtcttg ccgcagccag agggaccgag
     3541 cagcgcgaaa aactctccgg ccctgatgtc gagcgacagc cgatccaccg ctttgaagcg
     3601 gccgaacttc ttgaccaccg cgtcgatacg cagcagcgga acgtcggttg tatatcccag
     3661 cggttcgggt ttcggtagtt cgtcagccat gatcctcgcc gcccatcatg gccgcacgct
     3721 agcggcggat cgggcccggc tcaac
//
LOCUS       DASWGM010000939         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_520443,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000939 DASWGM010000000
VERSION     DASWGM010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520443"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VGA15_25350"
     CDS             complement(<1..237)
                     /locus_tag="VGA15_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX9591052.1"
                     /translation="MSVDAINATAATGIDPSRARIAGAIKQAASTTGASFEYLLATAK
                     MESNFDPKASASTSSARGLFQFIDQTWLGTVKEAG"
     gene            complement(485..925)
                     /gene="hisI"
                     /locus_tag="VGA15_25355"
     CDS             complement(485..925)
                     /gene="hisI"
                     /locus_tag="VGA15_25355"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX9591053.1"
                     /translation="MSTSQDNCEDSNDIEEGLTLQPKFDASGLVTCVATDAATGDVLM
                     VAHMNEEALRKTIESGEAWYFSRSRNALWRKGETSGHVQRVVEMRLDCDQDAIWIKVE
                     QTGAACHTGRRSCFYRKVDAGEGGARLTFVDADKLFDPDKVYRK"
     gene            complement(956..1711)
                     /gene="folE"
                     /locus_tag="VGA15_25360"
     CDS             complement(956..1711)
                     /gene="folE"
                     /locus_tag="VGA15_25360"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX9591054.1"
                     /translation="MDALIKPLRPSKPSDLKPSDLKPSDVKPSDAKPSDSRIAEIRPA
                     ELDPAEFLAAAVRADQPRPSRAEAEAAVATLLGYIGENPDREGLLDTPRRVVEAYDEL
                     YQGYHQCPAEVLNRTFGETAGYDDFVLIRDIEFTSQCEHHMMPFYGRAHIAYTPVERV
                     VGLSKLARLTDIFARRLQTQEHLTAQIAAAIDEVLKPRGVAVMIEAEHTCMSVRGVAK
                     HGASTFTSRFTGMFRDNPAEQARFMSMVRGPQR"
     gene            1956..2411
                     /locus_tag="VGA15_25365"
     CDS             1956..2411
                     /locus_tag="VGA15_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX9591055.1"
                     /translation="MLNDIYNKRIIELAGNIPRLGRLDTPDASATAHSKLCGSTVKID
                     LRMDGAVVTDFAHDVKACALGQASSSIMARHVVGSTASELRELRETVRKMLKENGKPP
                     QGKWADIALLEPVRDYKARHASTMLTFDAVVDAIGQIEAKTKQPVAAQG"
     gene            complement(2423..3361)
                     /locus_tag="VGA15_25370"
     CDS             complement(2423..3361)
                     /locus_tag="VGA15_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591056.1"
                     /translation="MRFVKSLDFRGWALLGAAALCIAWPGATATLGASARAGVTLEER
                     KMPMRFSWIACQLNCRGAGSGWVSAVGIVTADSPQDFDEFARGRQLNGATIVLDSSGG
                     SVNDSIALGRRWRNLGALTAVGISAATPTPQGARASVAPEAYCESMCVFLLLSGKVRY
                     VPEAAHVRVHQIWMGDRADDAKAASYSAQDMMIVERDIGRLAKYTFDMGGAGDLLSMA
                     LSVPPWEELHELSREELRLTNLVTTEAVAEVLPQLDGPNTPVAELAAKPVQDRFASET
                     VTAEKPQPAQPVSTKSTKTAEASVPGGVVAVVAPAQ"
     gene            3605..3934
                     /gene="yidD"
                     /locus_tag="VGA15_25375"
     CDS             3605..3934
                     /gene="yidD"
                     /locus_tag="VGA15_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX9591057.1"
                     /translation="MKYSHYADCAGTVLRIPRHAGRAMIWLYRHTLSPLVGYNCRHLP
                     TCSVYGDEAIERYGLWAGGWMTLARLLRCQPWGTSGIDNVPLTRPPGARWYLPWRFGR
                     WRGVNAP"
     gene            complement(4078..>4341)
                     /locus_tag="VGA15_25380"
     CDS             complement(4078..>4341)
                     /locus_tag="VGA15_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591058.1"
                     /translation="AYQPKYAELFRRAQPMDFGIGYRWRSFESNLLLSVQLPSDGSGS
                     ADATSSAPAAEPAQPPQPKPPRPRKPRPPTFLEPPPDFFRFWR"
BASE COUNT          781 a         1381 c         1395 g          784 t
ORIGIN      
        1 gcctgcttcc ttgacggtgc cgagccaggt ctggtcgatg aactgaaaca gcccgcgcgc
       61 cgacgaggtc gaggcgctgg ccttcggatc gaaattggat tccatcttgg cggtggcgag
      121 cagatattcg aagctggcgc cggtggtgct ggcggcctgc ttgatcgcgc cggcaatccg
      181 cgcgcgcgac ggatcgatgc ctgtcgcggc ggtggcgttg atcgcgtcga ccgacatctt
      241 gaggtgcccc gctccctgcg aaacgactta ctgagcaccc aatcgcgccc atgcccgacg
      301 tcacactggg atgattatgg ttaacggagt gttaaggagg aatcgtgccc cggatgctgc
      361 gcagcgcgcc gcactggcgg cgtggtgcgc tgctgatccg gggcccacaa gtcccgggtg
      421 gtcatgggtc ccggctctgc ggagcagcgt gaagaacgct gcaccgcgtc cgggacacgg
      481 aaaatcactt tcgataaacc ttgtcaggat cgaacagctt gtccgcatcc acgaacgtca
      541 accttgcccc gccctcaccc gcgtcgacct tgcgataaaa gcacgagcgc cggccggtgt
      601 ggcaggccgc accggtctgt tcgaccttga tccagatcgc gtcctgatcg cagtccaggc
      661 gcatttcgac cacgcgctgg acatggccgg aggtctcgcc ctttcgccac agcgcgttgc
      721 gcgagcggct gaaataccac gcctccccgc tttcgatggt cttgcgcagg gcctcctcgt
      781 tcatgtgcgc gaccatcagc acgtcgccgg tcgccgcgtc ggtcgcgacg caggtcacga
      841 ggccggaggc gtcgaatttg ggctgcaacg tcagcccttc ctcgatgtca ttgctgtctt
      901 cgcaattgtc ttgggatgtg gacacgggca gccctcgcga aatctgaacc gagggttagc
      961 gctgcggccc tcgcaccatg gacatgaaac gcgcctgctc cgccggattg tcgcggaaca
     1021 tgccggtaaa gcggctggtg aacgtcgagg cgccatgctt ggcgacgccg cgcaccgaca
     1081 tgcaggtatg ctccgcctcg atcatcaccg caacgccgcg gggtttcagc acctcgtcga
     1141 tcgcggccgc gatctgggcg gtcagatgct cctgggtctg caggcggcgt gcaaagatat
     1201 cggtgagccg cgccagcttc gatagcccca ccacccgttc caccggcgta taggcgatat
     1261 gggcccggcc gtagaacggc atcatgtggt gttcgcattg cgaggtgaat tcgatgtcgc
     1321 ggatcagcac gaaatcgtca tagccggcgg tctcgccaaa ggtgcggttc aacacctccg
     1381 ccggacactg atgatagccc tgatagagct cgtcataggc ttcgaccacc cggcgcgggg
     1441 tatccaggag gccctcgcgg tcggggtttt cgccgatata gcccaagagg gtcgcgaccg
     1501 ccgcttccgc ttcggcgcgc gagggacgcg gctggtccgc gcgcacggcg gcggcgagaa
     1561 attcggcagg atcgagctcg gccggacgga tctccgcaat cctcgaatcc gaaggttttg
     1621 cgtccgaagg cttgacgtcg gaaggcttga ggtctgaagg cttgaggtct gaaggcttgc
     1681 ttgggcgtag cggtttgatc aatgcgtcca tgtcaactcc gttcgaccga cccttaaggc
     1741 cggagtgtca ccgggcagac ggggcggttg gccgccggac gcctgagtcc caaaagtaca
     1801 aacaccgcgg cggccgaact agcttctatt cgggccgcca tgggaaacgg ctggactgta
     1861 tttccgccct ttctacccat atatagggct actgacagcg gtcgccaaga gtgattgcca
     1921 agcgcgatcg ccccgcaagc ccgagacccc atctgatgct gaacgacatc tataacaagc
     1981 gcatcatcga actggccggc aatatacccc ggctcggccg gctggacacg cccgacgcca
     2041 gcgccacggc gcactccaaa ttgtgcggct cgaccgtcaa gatcgacctt cggatggacg
     2101 gagcggtggt caccgacttt gcccatgatg tgaaggcctg cgcgctcggc caggcctcat
     2161 cctcgatcat ggcgcgccac gtcgtcggct cgaccgcgag cgaactgcgc gaattgcgcg
     2221 agaccgtgcg caagatgctg aaggaaaacg gcaagccccc gcaaggcaaa tgggccgata
     2281 tcgccctgct cgagccggtg cgcgactaca aggcgcggca tgcttcgacc atgctgacct
     2341 tcgacgccgt ggtcgatgcc atcggccaga tcgaggccaa gaccaagcag ccggtcgcgg
     2401 cacagggtta gagttcgcgg gcctactgcg ccggcgccac cacggcgacg acgccgccgg
     2461 gaacgctggc ttccgcggtc ttggtggatt tggtcgacac aggctgcgcg ggctgcggct
     2521 tctcggccgt caccgtctcg ctcgcgaaac gatcctgaac cggcttggcc gcaagctcgg
     2581 ccaccggtgt gttcgggccg tccagctggg gcagcacctc ggcaacggcc tcggtggtaa
     2641 cgaggttggt cagccgcaat tcttcccggg acagttcatg cagctcttcc cacggcggca
     2701 cgctcagcgc catcgacagc aggtcgcccg ctccgcccat gtcgaaggtg tatttggcga
     2761 gccgcccgat gtcgcgttcc acgatcatca tgtcctgggc gctgtagctc gccgccttgg
     2821 cgtcgtcggc gcggtcgccc atccagatct gatgcaccct gacatgcgcg gcctcgggca
     2881 cgtagcgcac cttgcccgac aacagcagga acacgcacat cgactcgcaa taggcttcgg
     2941 gcgcgacact cgcacgcgcg ccttgcgggg tcggggtcgc ggcactgatg ccgacggcgg
     3001 tcagcgcgcc aagattgcgc cagcgccggc cgagcgcgat cgagtcgttg accgaaccgc
     3061 cgctggaatc cagcacgatg gtggcgccgt tgagctggcg tccgcgcgcg aattcgtcga
     3121 aatcctgcgg gctgtccgcg gtcacgatgc cgaccgcgct gacccagccc gagcctgcgc
     3181 ccctgcaatt cagctggcac gcgatccaac tgaatcgcat cggcattttg cgttcttcga
     3241 gggtgacgcc ggcacgtgcc gaagcgccga gcgtagcggt tgcgccaggc catgcaatgc
     3301 acagggcggc ggcgccgagg agcgcccagc cgcgaaaatc gagtgacttc acaaacctca
     3361 atttggttcc ttctcctagc tcccagccga gcagtggtag cggccccagt tcagcgccaa
     3421 aatgattctc gtcacacagg cgttatgaaa gggtaacttc acgacaacga agcgtctatt
     3481 aacatttgct gcaccgccct caaaaaaatg cgatatttgg gcgggtgtca ctcaattgca
     3541 tgcctcgcac ggttccgtgc atcggaaccg ggcaaataca ggctcaacaa cgggcacggc
     3601 gcctatgaaa tattcgcatt atgcggactg cgccgggacg gttttgcgaa ttccgcgcca
     3661 tgcgggacgc gcgatgatct ggctttaccg gcacacgctg tcgccgctgg tcggttacaa
     3721 ttgccgtcac ctgccgacct gctcggtcta tggcgacgag gcgatcgagc gttacgggct
     3781 gtgggccggc ggatggatga ccctggcgcg gctgttgcgc tgccagccct ggggcacgtc
     3841 ggggatcgac aacgtgccgc tgaccagacc gccgggcgcg cgatggtatt tgccgtggcg
     3901 tttcgggcgg tggcgcgggg tcaacgcgcc atagagtttg cttcagccag gctgaagcaa
     3961 actcgtcgct tatttacgag ccaaaacgtg cggtggtgag gaattttgtt tgaggcgtaa
     4021 acaggtatcc gccccgcatc aagtgcgggg caggctttca ctcgaacaca gggtagctta
     4081 gcgccaaaac ctgaagaaat ccgggggcgg ctcgagaaag gtcggcggac gcggcttccg
     4141 gggacgcggt ggtttgggct gcgggggctg cgcgggttcg gccgctggcg ccgatgacgt
     4201 cgcgtcagcg ctccccgaac cgtccgaggg aagctggacc gacagcagca ggttcgattc
     4261 aaacgatcgc caccgatagc ctatgccgaa atccatcggc tgagcccgcc tgaacagctc
     4321 ggcatatttc ggctggtatg c
//
LOCUS       DASWGM010000940         3892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1619163,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000940 DASWGM010000000
VERSION     DASWGM010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619163"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..2213
                     /locus_tag="VGA15_25385"
     CDS             303..2213
                     /locus_tag="VGA15_25385"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEX9591059.1"
                     /translation="MSSEIPGHFRNTTRTRLRPDVPRAAEPRRRPARPDQKAPKPPRQ
                     KERVRKTDLGTILLHWILVITLTLSIGTGLRIAMDSPEHSWLVAFDSFLPLYTVWTLH
                     IPAALTLVAVSVAYAAYIWRGGLMRRIRFDKMRLKGLGNRSKQVRWGSINIILYWVLF
                     ATMIMEITTGTLLWLGYGRGIWVEMHMWGTWIIFGYAAAHVLAHFALGGFNQLARVLR
                     PTKLGPRPTPFDPMDLVAELLDPENAANSQAEPTPKSRWIFRLSPLVVALAAATAGIA
                     IAITVDGASEDTFFVRRIAKADRPMLDGDLSDGVWRTVRPFTMFTALGGNYDGKGEAA
                     VEIRAVHDGEWAYFCFMWDDPTRSLKHLPLVKRADGWHVLHDRYDLGDERSYYEDKFS
                     ALFTKLPIVIPGDRTYHAGPTPIEGKPSTLSQRGLHFTPAPGLAVEVWQWKASSGGLL
                     GWVDKNHFDMPTEASRAESEGHVPYRGGFIQDPRTAELYSDNFERQPVGGYERPIQPK
                     RLPRNWKATYEALGRIDLDPDHSDAEASRWWMNMSESEPYSAVLDAEIPVGTMIPGSL
                     ILVDDIDRRADVIGMARWAAGHWTLEVARKLAAEAPDVPISTGTYLRVAVFDHSQIYH
                     TRHIRPIRLELE"
     gene            complement(2307..2684)
                     /locus_tag="VGA15_25390"
     CDS             complement(2307..2684)
                     /locus_tag="VGA15_25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9591060.1"
                     /translation="MTATRPPGGSVFLVEDEVMIRMMVADMLEELGYSVAAEAGEINE
                     AIRLAQCTEFDLAILDVNVNGKVISPVAELVKARNRPFIFATGYGSSGLPEEYRDRPA
                     LQKPFQLETLARMIESALKSATV"
     gene            2974..3624
                     /locus_tag="VGA15_25395"
     CDS             2974..3624
                     /locus_tag="VGA15_25395"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX9591061.1"
                     /translation="MPPAQNPPEKMMFQLNLRRRGISDQAVLRVMEEIPRDLFVEVAD
                     RADAYRDSALAIACGQTISQPFVVAYMTEQLQLQKHHRVLEIGTGSGYQAAILSRLCS
                     QVLTVERFRTLADNARLRLKKLGCGNVEVTLGDGFDIPASAGNFDRIMVTAAMQQIPD
                     SLTQRLEPGGILIAPVGPPHGVQTLIRIISTDTGFKRRELVDVRFVPALPGIAREL"
BASE COUNT          720 a         1197 c         1212 g          763 t
ORIGIN      
        1 accgcgcata aattcgtcga tctttcttca atcacatggt cttgccgcac tgcatcagcg
       61 aattgcctaa ttccgacccc gaaattcgct caatcaaaat atacaaatca acactagttg
      121 aaggggtcat ccatatccgc actgtgaaga cgtagctgat cctgcgtctg gagcgtcctt
      181 ctgcgttacg gcgcggaatg gatcgcgctt gtcacaatgc caccagatga cttcgagttg
      241 tagcgatgga ggttcgatgg accggcagcg gcatcagcga acttcggagt caggtaaagc
      301 cgatgtcttc tgaaatccca ggccacttcc gcaacacgac ccgcacgcgc ctgcgtcccg
      361 atgtccctcg cgccgcggag ccgcggcgca ggccggcgcg accggatcag aaggcgccaa
      421 agccgccgcg acaaaaggag cgcgtacgaa agaccgacct cggaacgatc ctgctgcatt
      481 ggattctggt gatcacgttg acgttgtcga tcgggaccgg actccggatc gcgatggatt
      541 caccggagca ttcgtggctc gttgcgttcg acagtttcct gccgctttac accgtgtgga
      601 cgctgcatat tccggcggcg ctgaccctcg tcgccgtgtc ggtcgcctac gcggcctata
      661 tctggcgcgg cggcctgatg cgccggatcc gttttgacaa gatgcggctg aagggattgg
      721 gcaaccgcag caagcaggtg cggtggggtt cgatcaatat catcctgtac tgggtgttgt
      781 tcgcaaccat gatcatggag atcacgaccg gcaccttgct ctggctcggc tatgggcgcg
      841 gtatctgggt cgagatgcac atgtggggca cctggatcat tttcggttat gcggcagccc
      901 atgtcctggc gcactttgcg ctcggcggct tcaatcagct cgcccgcgtg ttgcggccga
      961 ccaagctcgg cccgcggccg acgccgttcg atccgatgga tttggtggcg gaactgcttg
     1021 accccgaaaa cgcggcgaac agccaggccg agccgacgcc gaagagccgg tggatttttc
     1081 gcctcagccc gcttgtggtg gcattggcgg ccgcgaccgc gggaatagcg atcgccataa
     1141 ccgtcgacgg ggcgtccgag gacaccttct ttgtccggcg catcgccaag gccgaccgtc
     1201 cgatgcttga cggcgatctg tccgacgggg tctggcgaac ggtcaggccg ttcacgatgt
     1261 tcaccgcact tggcggcaat tatgacggca agggcgaagc ggcggtcgag atccgcgcgg
     1321 tccacgatgg cgaatgggct tacttctgct tcatgtggga tgacccgacc cgatcgctga
     1381 aacatcttcc gctggtgaaa cgggccgacg gctggcatgt cctgcatgat cgatatgatc
     1441 tcggtgacga gcgatcctat tacgaggaca agttctccgc gctgtttacc aagctgccga
     1501 tcgtgattcc cggcgaccgc acctaccacg ccggaccgac gccgatcgag ggcaagccgt
     1561 cgacgttgtc gcaacgcggt ctgcacttta ccccagcacc cggtctcgcc gtcgaagtct
     1621 ggcaatggaa ggcgtcgagc ggcggcctgc tcggatgggt cgataaaaac catttcgaca
     1681 tgccgaccga ggcgtctcgg gccgagtcgg agggccatgt gccctatcgc ggcggcttca
     1741 tccaggatcc acgcacggcg gagctctaca gcgacaattt cgagcgccaa cccgtcggcg
     1801 gctatgagcg gccgatccag ccgaaacggc tgcccaggaa ctggaaggcg acctatgagg
     1861 cgcttgggcg gatcgacctc gatcccgacc acagcgatgc cgaagcgtcg cgatggtgga
     1921 tgaacatgtc ggaatccgag ccctattcgg ccgtgctcga cgccgagatt ccggtcggaa
     1981 ccatgatacc gggatcgctg atcctggttg acgatatcga tcggcgcgct gatgtcatcg
     2041 gcatggctcg ctgggcggcc ggccactgga cgctggaagt ggcgcgcaag ctggcggccg
     2101 aggcccccga tgtcccgatt tccaccggga cctacctgcg ggttgcggtt ttcgaccatt
     2161 cccagatcta ccacacccgt cacatccgcc cgattcgcct cgagcttgag tgatgatgtg
     2221 ttggcgtcct ccccggatga gcgcaattgc actcatccgg ggatgacgtc gattggtcgg
     2281 ctgtcaattc ggttttcgtc caaaacctag accgtcgcgc tcttgagcgc gctctcgatc
     2341 atccgcgcca gggtttcgag ctggaaaggt ttctgcagtg ccggacggtc gcgatattct
     2401 tccggaaggc ctgaggagcc atagccggtc gcgaagatga acgggcgatt gcgcgccttg
     2461 accaattcgg cgacaggaga aatcaccttg ccgttgacgt tgacgtcgag aatagcgagg
     2521 tcgaactcgg tgcattgcgc cagcctgatc gcctcgttga tctcgccggc ctcggcggcc
     2581 acgctgtagc cgagctcctc cagcatgtcc gcgaccatca tcctgatcat cacctcgtcc
     2641 tcgacgagga acacagaacc gcccggcggc cgcgtcgcag tcatgataat gtccttgccc
     2701 gtcccaagcc tcaaggttcg caaataacgc ccatcggcgc aatgtcaacg tgtgaacacg
     2761 caacctggct ggccgccatc gcgccgtggc ggggcaggga gcaggattga ttattgattg
     2821 cccgcttacc aacgccaagg tgctatcccg ggttccacag ccggaacacc gattcgtggg
     2881 gaagttttcc ttaaggcggc ggtgcatatt gcatcggccg gttgggccga ggcgcgctag
     2941 agtggcctga tacctgatct tgacgagatt gccatgccgc ccgctcaaaa tccgccggaa
     3001 aagatgatgt tccagctcaa cctgcggcgg cgcgggatca gcgatcaggc ggtgctgcgc
     3061 gtcatggaag agattccgcg cgaccttttc gtggaggtgg ccgaccgcgc cgatgcctat
     3121 cgcgacagcg cgctcgccat cgcctgcggg caaaccatca gccagccctt tgtggtggct
     3181 tacatgaccg agcagttgca gctgcagaag catcaccgcg tgctcgagat cggcaccggc
     3241 tccggttacc aggcagcgat actgtcgcgg ctttgcagtc aagtgctgac ggtcgagcgc
     3301 ttccggactc tggccgacaa tgcccggttg cggctcaaga agctcggctg cggcaatgtc
     3361 gaggtgacgc tgggcgacgg tttcgacatc ccggccagtg ccggcaattt cgaccgcatc
     3421 atggtcaccg cggcgatgca acaaattccc gactcgttga cgcagcggct cgagccgggc
     3481 ggaatcctga tcgcgccggt cggtccgccg cacggcgtcc agaccctgat ccgcataatc
     3541 agcaccgaca ccggtttcaa gcgcagggag ctggtagacg tgcgtttcgt gccggcgctg
     3601 ccgggcattg cccgggagct atagggtagg gagcaacgcc agacgctgtt cgggacgcgc
     3661 gcaccggaca gcgcaagcgt gctgccggga gcttgtgcgc ctcgccatga ggggttgccc
     3721 caggccgctt cgactacccg tcaaggcccc gtccgaaggg ttaagcggtt gtttactggg
     3781 ggtgtgttta ctcaaaacag tatcaagttg cgtacgagtg agtaaccatg tcccgaatcg
     3841 tcgagttgct ttgctcgcgc cgcttgccgc acgtcgcggt gctggcgctg at
//
LOCUS       DASWGM010000941         7163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2202693,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000941 DASWGM010000000
VERSION     DASWGM010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202693"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..828
                     /locus_tag="VGA15_25400"
     CDS             181..828
                     /locus_tag="VGA15_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9591062.1"
                     /translation="MPEVIFTGPAGRLEGRYHPAKQKNAPIAMVLHPHPQFHGTMNHQ
                     IIYQCYYAFAHRGFSVLRFNFRGVGRSQGSFDHGTGELSDAASALDWAQTINPEARAC
                     WVAGFSFGAWIGMQLLMRRPEVEGFISIAPPANLYDFSFLAPCPSSGLIVHGEKDAVV
                     PPKDVNTLVEKLKTQKGIVIDQQIIPGANHFFDGKLQPLMETVTGYLDMRLANVR"
     gene            complement(1013..1687)
                     /locus_tag="VGA15_25405"
     CDS             complement(1013..1687)
                     /locus_tag="VGA15_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9591063.1"
                     /translation="MAAHLKLISHKLCPYVQRAVIALAEKGVAFERIDIDLANKPEWF
                     LAISPLGKTPVLQVGDTAIFESAVILEYLEETQPKPLHPADALARAEHRGWIEFGSAV
                     LNDIAGFYAAPDQATFNAKTSQLERRFARLETRVAAAPWFDGEGFSLVDAVFGPVFRY
                     FDVFDEIADFGILAGKPKQKCWREALAARPSVRTAVSADYPALLRSFIDRRNSWLSGL
                     QARAAA"
     gene            complement(1782..2885)
                     /locus_tag="VGA15_25410"
     CDS             complement(1782..2885)
                     /locus_tag="VGA15_25410"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX9591064.1"
                     /translation="MMLTAIGLMSGTSLDGVDVALIETDGRRVKAFGPSGYRPYTDAE
                     RGLLRQALAEAVDLPQRDARPGILREAERAVTAAHAEAVAAFTAQHRIAREDIDIVGF
                     HGQTVLHRPAQKMTVQIGDAAALARAIHIPVMHDFRAADVEAGGQGAPFVPVYHRALA
                     QTLQREGPIAVVNIGGVSNITYIDGADTLIACDTGPGNALLDDFMFRTMGQRFDCEGR
                     MAAQGAVDVAWVMQALAHPFFALPPPKSLDRNDFASQILRGVSPADGAATLTAFTAEA
                     IARIAPLLPKQPRSWIVAGGGARNLTMLRMLRERLEPATVETAEGLGWSGDAIEAQAF
                     GFLAARGLKGLPLSYPATTGVPFPMTGGVIARP"
     gene            3052..4305
                     /gene="tyrS"
                     /locus_tag="VGA15_25415"
     CDS             3052..4305
                     /gene="tyrS"
                     /locus_tag="VGA15_25415"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX9591065.1"
                     /translation="MTAFKSDFLNILQERGFIHQCSDFEGLDALAAKGQATAYVGYDC
                     TAPSLHIGNFLTMMMLHWLQQSGNKPITLMGGGTTMVGDPSGKDESRALRTVEEIEAN
                     KASIRGVFAKVLRYGSGPSDAVMLDNAEWLTKLNWIEMLRDIGRHFSVNRMLTMDSVR
                     LRLEREQEMSFIEFNYMVCQAYDFVELSRRVGCRLQMGGSDQWGNIVNGVDLGRRMGT
                     PQLFALTTPLLTTASGEKMGKTARGAVWLNADEFSPYDFWQYWRNVEDADVVRFLKLF
                     TILPMSEIARLAALRGAEVNEAKKVLATEATALLHGRDAASVASETARQTFEEGAISQ
                     SLPTATVPRSILENGLSVLEAYVLLHLAISKGEARRQIKNGGLRVNDLVVVDENMTLT
                     PADLTPEGVIKLSIGKKRHILLRPA"
     gene            4361..5608
                     /locus_tag="VGA15_25420"
     CDS             4361..5608
                     /locus_tag="VGA15_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9591066.1"
                     /translation="MDQNTPRENPETKSPQPARMALNVLALCFALSVFGRGLAESFAV
                     FLKPISESFGWDRAQVVSVYSLAALAGGLTAPLVGRLFDRSGPRTVYSLGLALLGGAF
                     LVAAHAQHLWQFQLSIGLCVGIGIAFIGNVPNSILLGRWFGPRLPTAMAIVYSATGAG
                     VLILLPVSQVLIDRFGWRGAYQIFGGGALFLLLPLLLMPWRLFSTGSPHVVKKADPDF
                     VDEGWTLPGAMRHHAFWALFSTFFFTAIGMYAISPQVVAYLIDAGFPPLQAATAWGFS
                     GVVLLFGMLGISWLDGIIGRRPSVLFSYALSIAGILMLWGLQWYPNFWMLTGFVVCFG
                     SMIGSRGPLLTATAMKIFRGKRIGTIYGTISIGSGLGSALGSWSGGLIHDWAHSYNPL
                     IAFALVSVLLGMIPFLVVPALRR"
     gene            5703..>7163
                     /locus_tag="VGA15_25425"
     CDS             5703..>7163
                     /locus_tag="VGA15_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX9591067.1"
                     /translation="MSLLSRLKIRTKLASMVVLSALTVCAIIAVSASLSERRMVEDRV
                     AQLRAAVDLMVGMAQTLEDEVTAGKMTHAEAQAQFRLRGRKMTFGNGQGYPNVYNADS
                     SVLLNGGNPSIEGKITGATDSNGVLISKAQIDAARSKPEGNVTSYLYPRPGQTVPSKK
                     LAFVRHFAPWNITISYGVYVDDLDADVNALLWRLGVLGAGVIALMALLSWLIARDILG
                     ALDRQRTRTQRIATGSLDQAVEETERGDEIGRMAETLEMLRQTALTARTLEAEQIATK
                     QQGEQEKREALIALADRFDASVGRLVGMMASGSTELEATAQSMTGTAERTNQQATVVS
                     SAAAEASARVQTVATAAEELSSSISEISRQVAQSSNIAARAVDSARRTDSIVRALADG
                     AQQIEHVAELISSIAGQTNLLALNATIEAARAGEAGRGFAVVAAEVKSLASQTAEATK
                     EIGTRITQIQSATKEAVEAIQGITATIEEVSAIATTI"
BASE COUNT         1329 a         2260 c         2239 g         1335 t
ORIGIN      
        1 gctttttgcc cctcgcctta tgctagaacg cggcctccag ttcaccgagc gcccgttcgc
       61 ttaagcccaa cggcgcctga tccgcccctt tatctctgct gagattcatc tcgcgaaaca
      121 gccagatgga gccggcacaa aggcggttgg ttttcgccga gggatcatca agggatcacg
      181 atgcctgaag tcattttcac cggccccgca ggccgcctcg aaggccgtta tcatccggcc
      241 aagcaaaaga acgcgccgat cgcgatggtg ctgcacccgc atccgcagtt tcacggcacg
      301 atgaatcatc agatcatcta ccagtgctac tacgcgttcg cgcatcgcgg attttcggtg
      361 ctgcgcttca actttcgcgg cgtcggccgc agccagggct cgttcgacca cggcaccggc
      421 gaactttcgg atgccgcctc cgcgctcgac tgggcgcaga ccatcaatcc cgaggcgcgc
      481 gcctgctggg tcgccggctt ctcgttcggc gcctggatcg gcatgcaact gttgatgcgc
      541 cggcccgagg tcgaaggttt tatttcgatc gcaccgcctg ccaatctcta tgacttctcg
      601 ttcctggcgc cttgcccgtc ctcgggactg atcgtgcatg gcgagaagga tgccgtggtg
      661 ccgcccaagg acgtcaacac gctggtcgag aaactgaaaa cccaaaaggg catcgtgatc
      721 gaccagcaga tcatcccggg cgcgaaccat ttcttcgacg gcaagctgca gccgctgatg
      781 gagaccgtca ccggctatct cgacatgcgg ctcgccaacg ttcgatagtc tggctcgtgc
      841 cccggacgcg gtgcatcacc atcagcgcgt ttacgcgcgt ctgcgacgcg ccatggtggt
      901 gcgccgcaga gccggggcct atatctccag ttggttcctt cggcagtaat aggtcccggt
      961 tctgcgaagc agcgctgcgc gctgcatcgc gcccgggaca cgcggcctat ggtcacgcgg
     1021 cggcgcgggc ctgcaggcct gacagccatg aattccgacg gtcgatgaag ctgcgaagca
     1081 gcgccggata atcggcgctg acagccgtgc gcaccgacgg ccgcgctgcc agagcctcgc
     1141 gccagcattt ttgcttcggc ttgcctgcga gaattccgaa gtcggcaatt tcgtcgaaca
     1201 cgtcgaaata gcgaaacacc ggaccgaaca ccgcgtcgac cagcgaaaaa ccttcgccgt
     1261 cgaaccatgg cgcggccgcg acccgcgtct cgagccgcgc aaagcgccgc tcgagttgcg
     1321 atgttttggc gttgaaggtt gcttgatcgg gcgccgcgta aaagcctgca atgtcgttaa
     1381 gcacagccga gccaaattcg atccagccgc gatgctcggc gcgagccaaa gcatcggccg
     1441 gatgcagcgg ttttggttgg gtttcctcca gatattccag gatgacggcc gactcgaaga
     1501 tcgcggtgtc gccgacctga aggaccggtg ttttaccgag cggcgaaatc gccagaaacc
     1561 actccggttt gttggcgaga tcgatatcga tccgctcgaa ggccacgccc ttctcggcga
     1621 gcgcgatcac cgcgcgctgc acataggggc aaagcttgtg gctgatcagt tttaggtgtg
     1681 cggccatctc gggggttctc gcagttacat gcatttgcat taaactacat gcgcctgcat
     1741 gaaactgtca agctcgatgc aattgcatcg accgcccccg ctcacggtcg cgcgatcacc
     1801 ccgccggtca tcgggaacgg gaccccggtg gtggcgggat agctcagcgg caggcccttc
     1861 aagccccgcg ccgccagaaa cccaaaagcc tgcgcctcga tggcgtcgcc ggaccagccc
     1921 agtccctcgg cggtctcgac ggtggctggc tccagccgct cgcgcagcat ccgcaacatc
     1981 gtgagattgc gggcgccgcc gccggccacg atccagctcc gcggctgctt cggcaacagc
     2041 ggagcgatcc gggcgatagc ttcggcggtg aaggccgtca gggtcgccgc accgtcggcg
     2101 ggagacaccc cgcgcaaaat ctgcgaggca aaatcgttcc ggtccagcga tttcggtggc
     2161 ggcaatgcaa agaacggatg cgccagcgct tgcatcaccc acgccacgtc caccgcgccc
     2221 tgcgctgcca tgcggccttc gcaatcgaac cgctggccca tggttcgaaa catgaaatcg
     2281 tcgagcagcg cgttgccggg cccggtatcg caggcgatca gggtatcggc gccatcgata
     2341 taggtgatgt tggagacgcc accgatatta accacggcga tcggcccctc gcgctgcagc
     2401 gtctgggcca gcgcgcggtg atagaccggc acgaagggcg cgccctgccc gccggcctcg
     2461 acatcggcgg cgcggaaatc atgcatcacg gggatatgaa tggctctcgc cagcgccgcc
     2521 gcatcgccga tctgcacggt cattttttgc gcgggccggt gcagcacggt ctggccgtga
     2581 aagccgacga tatcgatatc ctcgcgggcg atgcgatgct gcgcggtaaa ggcggcgacc
     2641 gcttccgcat gcgcggccgt gacggcgcgc tcggcctcgc gcaggatgcc tggccgcgca
     2701 tcgcgctgcg gcaggtcgac cgcctccgcc agggcctggc gcagcagacc gcgttcggcg
     2761 tcggtgtagg gcctgtaacc cgacgggcca aacgccttga cccgcctgcc gtcggtctcg
     2821 atcaacgcga catcgacccc gtcgagcgag gtgccgctca tcagaccgat tgccgtcaac
     2881 atcatcgcaa tagtgccttt gcgggcgcac tgccgctgac gcccagcttg tatcaaacca
     2941 gcacatctta taatgccaca gcgcgcccgg cagcggcagc ctattccgct atggttccgc
     3001 aactcgttaa gaatagcgtg aaaaagtaag aatgatcaga gtcgcacaca aatgactgca
     3061 tttaaatccg attttctgaa tatcctgcag gagcgtggct tcatccacca atgttccgat
     3121 ttcgaaggcc tcgacgcgct ggccgcgaaa ggccaggcaa cggcctatgt cggttacgac
     3181 tgcaccgcgc cgtcgcttca tatcggcaac ttcctcacca tgatgatgct gcattggctg
     3241 cagcagagcg gcaacaagcc aatcacactg atgggcggcg gcaccaccat ggtcggcgat
     3301 ccctccggca aggatgaatc gcgcgcgctg cgcaccgtcg aggagatcga agccaacaag
     3361 gcgagtatcc gcggcgtctt cgccaaggtg ctgcgctacg gttccggccc gagcgacgcg
     3421 gtcatgctgg acaatgcgga atggctgacc aagctgaact ggatcgagat gttgcgcgac
     3481 atcggccggc atttctcggt caaccgcatg ttgacgatgg actcggtgcg gctccgtctc
     3541 gagcgcgagc aggagatgag ttttatcgaa ttcaactaca tggtctgcca ggcctacgat
     3601 ttcgtcgaac tgtcgcggcg cgtcggctgc cgattgcaga tgggcgggtc ggaccagtgg
     3661 ggtaacatcg tcaacggcgt cgacctcggc cgccgcatgg gaacgccgca attgttcgcg
     3721 ctgacgacgc cgctgctcac gacggcgtcg ggcgaaaaga tgggcaagac cgcccgcggc
     3781 gcggtctggc tcaacgccga cgagttcagc ccctacgact tctggcaata ctggcgaaac
     3841 gtcgaggacg ccgatgtggt aagattcctc aagctgttca cgatcctgcc gatgagcgag
     3901 atcgcgcggc tcgcggcgtt gcgaggcgcc gaggtcaacg aggccaagaa ggtgctggcg
     3961 acggaagcga ccgcgctgct gcacggccgc gatgcggcca gcgtcgcgtc cgaaaccgcc
     4021 cggcaaactt tcgaggaagg cgcgatctca caatcactgc ctaccgcaac ggttccacgt
     4081 tccattcttg agaacggact gagcgtcttg gaggcctatg tgttattgca tctggcaatt
     4141 tcgaagggcg aagcgcgccg ccaaatcaaa aacggtggct tgcgagtcaa cgatttagtc
     4201 gtcgtcgacg aaaacatgac gctgacgccg gccgacctca caccggaagg cgtaataaaa
     4261 ctttcgatcg gcaagaagcg ccacatcctg ctcaggcctg cataggcgca ttcgcccaag
     4321 acccctgctc ttcgcggccg aaaagcgttc cttggtgggc atggatcaaa atacgccgcg
     4381 cgagaatccg gagacaaaat cgccgcagcc ggcacgcatg gcgcttaacg tgctggcact
     4441 gtgttttgcg ctgtcggtgt ttggccgcgg tctcgccgag agttttgccg tgttcctcaa
     4501 gccgatctcg gagagcttcg gctgggaccg cgcccaggtc gtctcggtct actcgctggc
     4561 agcccttgcc ggcgggctga cggcgccgct ggtcggccgg ctgttcgacc ggtcgggccc
     4621 gcgcacggtc tattcgcttg gcctggcact gcttggcggg gcgtttctgg tcgccgccca
     4681 tgcgcagcat ctctggcagt tccagctcag cattggtctc tgcgtcggga tcggcattgc
     4741 gttcatcggc aacgttccga actcgatcct gctcggccgc tggtttggcc cgcggttgcc
     4801 gaccgcgatg gcgatcgtgt attcggcgac cggcgccggc gtattgatcc tgctgccggt
     4861 ctcgcaggtc ttgatcgatc gcttcggctg gcgcggcgcc tatcagatct tcggtggcgg
     4921 cgcgctgttt ctgctgttgc cgctgctgct gatgccgtgg cggctgttct cgaccggctc
     4981 cccgcacgtc gtcaaaaagg ccgatccgga ttttgtcgac gaaggctgga cgcttcctgg
     5041 cgccatgcgc caccacgcgt tctgggcgtt gttctcgacg ttcttcttca ccgcgatcgg
     5101 catgtatgcg atttcaccgc aggtcgtggc ctatctgatc gacgccgggt tcccgccatt
     5161 gcaggcggcg accgcatggg gtttcagcgg tgtggtgctg ctgttcggca tgctcggcat
     5221 cagttggctc gacggcatca tcggccgcag gccatcggtc ctgttcagct atgcgctttc
     5281 gatcgctgga attctgatgc tctggggcct gcagtggtat ccgaacttct ggatgctgac
     5341 gggattcgtc gtttgtttcg gcagcatgat cggctcgcgc ggcccgctgc tgacggcaac
     5401 cgcgatgaag atctttcgcg gcaagcggat tggtacgatc tacggcacca tttcaatcgg
     5461 cagcggcctg ggctcggcgc tcggctcctg gagcgggggc ctgatccacg actgggccca
     5521 cagctataat ccgttgatcg cgttcgcgct ggtcagcgtg ctgctgggga tgattccatt
     5581 cctggttgta cctgcgctgc ggcgctgagg ctcgcacagc cggtatcatg ttcacgttca
     5641 ctattggtaa gaaagttttt gatactccct ctgcaaacag tttttcacca ggaaggttga
     5701 aaatgagttt gctgagccgt ctgaagatcc ggaccaaact tgcgagcatg gtggtgctgt
     5761 cggcactgac cgtttgcgcg atcatcgcgg tctcggcttc gctgagcgag agacgcatgg
     5821 tggaggaccg ggtggcacag ttgcgcgccg cggtcgatct catggtcggc atggcacaga
     5881 cgctggagga cgaggtcacg gcaggaaaaa tgacccacgc cgaggcgcag gcccagtttc
     5941 gcctgcgtgg ccggaaaatg acgttcggca acgggcaagg ctaccccaat gtctacaatg
     6001 cagatagctc ggtgctgctg aacgggggca acccttcgat cgaaggcaaa atcaccggtg
     6061 cgacggattc caacggtgtg ctgatctcca aggctcaaat cgacgccgcc cggtcgaaac
     6121 cggagggaaa tgtcacgtcc tatctctatc cccgccccgg ccaaaccgtg ccctccaaga
     6181 aacttgcctt tgtacgccac ttcgcgccgt ggaacatcac catcagctac ggggtgtatg
     6241 tggatgatct cgatgcggac gtaaacgcat tgctgtggcg ccttggcgtg ctgggcgccg
     6301 gcgtcatcgc gttgatggcc ttgctgtcgt ggctgatcgc tcgcgacatc ctgggagcgc
     6361 tcgaccggca gcggactcgc acgcaacgca ttgcgactgg atcgctcgac caggcggtgg
     6421 aagaaaccga acgcggcgac gagatcggac gcatggcgga aacgctggag atgttgcggc
     6481 agacggcgct gacggcgcgg acgctcgaag ccgagcagat agcgacgaag cagcaaggcg
     6541 aacaggagaa gcgcgaggcc cttattgcgc tcgccgatcg atttgatgcc tctgtgggcc
     6601 ggctggtcgg aatgatggct tcgggatcga ccgaactgga agcgaccgcg caatccatga
     6661 ccggcacggc ggaacgcacc aaccagcagg cgacggtcgt cagctcggcg gcggcggaag
     6721 ccagcgcccg cgtgcaaacc gtcgccacgg cggcggaaga actgtcctcg tcgatcagcg
     6781 aaatcagccg tcaggtcgca caatccagca atatcgccgc gcgcgcggtc gacagcgcac
     6841 gccgtaccga cagcatcgtg cgcgcccttg ccgatggggc ccagcagatc gagcatgtgg
     6901 cggaactgat ttcaagtatc gcgggccaga ccaatctgct ggcgctcaac gccaccatcg
     6961 aagccgcccg cgcaggcgaa gccggcaggg gtttcgcggt ggtcgctgcc gaggtcaaga
     7021 gcctcgccag ccagaccgcg gaagccacca aggagatcgg cacccgcatt acccagatcc
     7081 agagcgccac caaggaagcc gtcgaggcga tccagggcat caccgccacg atcgaggaag
     7141 tcagcgcgat cgcaacgacg atc
//
LOCUS       DASWGM010000942         9733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1076962,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000942 DASWGM010000000
VERSION     DASWGM010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9733
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076962"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VGA15_25430"
     CDS             <1..779
                     /locus_tag="VGA15_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9591068.1"
                     /translation="DMPFEIANIQCENPEAAAHTRIGWFRSVSNIPRAFAVQSMVGEL
                     AHATNRDQKDMLLELIGSPRIVKLDSVKDLWNYGEPYDSYPIDTARLRRVVEVVADKG
                     GWGRTVPKGHGLGIAVHRSFVSYIATMVEVSVGDKGKLTVHQVDTAIDCGMFVNPERI
                     QSQIEGAAIMGLSLAKYGEITFKDGKVQQGNFDDFPVVRIDEAPLVTNVHIVPANADT
                     PPSGVGEPGVPPFAPALINAIFAATGKRIRALPIGKQLET"
     gene            complement(2348..2812)
                     /locus_tag="VGA15_25435"
     CDS             complement(2348..2812)
                     /locus_tag="VGA15_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591069.1"
                     /translation="MPVEKALSSLQRDDLRVVAAMIIPASEQYGVPGADDAAIQADIL
                     ATLDRDAELVREALDHLARLAAMPLANLGETVREAVARQFRASGGAAAATLTRVVLQC
                     YYRDDRVLRSLGLELRPPFPEGYALEQGDWSLLDPVKARPSMWRRAPSGKPD"
     gene            complement(2820..4418)
                     /locus_tag="VGA15_25440"
     CDS             complement(2820..4418)
                     /locus_tag="VGA15_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9591070.1"
                     /translation="MNDPVDVLIIGAGASGAAVAWSLAETKMHILCLEQGDWMNPAQY
                     PSVGRDWEARAYGDYSISPNIRARPEDYPVNDDNSPIKVVNFNGVGGSTIMYTAHFPR
                     LHPSDFRVRTLDGVAEDWPIDYDTLAPFFAENDRMMGVSGLAGDPAFPPTDPPMPPLP
                     LGRSGTRLAKAMNKLGWHWWPSDTTVATVDYEGRARCINLGHCTPACAQGAKASTDIT
                     YWPVALRAGVELRTHCRVREITTNGQGMATGVVYYDKDGVEQFRPAEVVVIACNGVGT
                     PRLLLNSASGRFPNGLANSSGLVGKNLMFHPYAQAYGYVDEPTDSNRAPPTCLWSKEF
                     YETDPSRGFVRGYAFQFGRGIGPVTEAVTSEAKGLLPWGAGHHAAFRRLNGHRLGLSA
                     ICEDLPEEHNRVTLDPILKDSHGIPAPRVDYTIGENSARMLEHGIARAKEILAVAGAT
                     DICVNCPILYGGWHLLGTARMGVDPERSVVNEWGRSHDVKNLFIVDGSVFVTSGGVNP
                     TSTIQAIALYVADQMKQRLANLFD"
     gene            complement(4581..5813)
                     /locus_tag="VGA15_25445"
     CDS             complement(4581..5813)
                     /locus_tag="VGA15_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9591071.1"
                     /translation="MVQAEARQRALPSIDVSNPKLYQEDAWRPLFATLRRDDPVHYCE
                     ASRFGPYWSVTRYDDIFTVELDHENYSSSSELGGIQVADQPKGQERASFIRMDPPGHT
                     AQRRTVAPIVAPSNLVNLEPLIRKRTADVLDALPRHETFDWVTRVSVDLTNMMLTTLF
                     DFPWADRERLTWWSDVAIANVDSPDAVVHSEDERFAELDKMGAYFRKLWDARAAAPPA
                     FDLVSMLAHSDATRNMPPREFIGTLGLLIVGGNDTTRNSMSGGLMALVENPEQFELVR
                     SRRDLIPNLVSEIIRYQTPVLHMRRTARNDAELAGRSIAKGDKVVMWYISGNRDEEKI
                     EHADQFIVDRAKPRHHLAFGAGIHRCVGDRLAEQQLRILWEEILQRDLRFEIMGPPQR
                     LYSNFIRGIRSLPVRIVN"
     gene            complement(6697..8067)
                     /locus_tag="VGA15_25450"
     CDS             complement(6697..8067)
                     /locus_tag="VGA15_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9591072.1"
                     /translation="MREELHRFFRAARGAGVRVSPAESIDSMKAVADVGFGDRGILRD
                     TLLLTLAKSQDEKQALGECFDLFFRQPEVKEEETAPEDAAESTQRDAAAGETPSGDAG
                     APSPELGELAQMLLSRDRNAIAAAMANAANAASLSDIRYFTQRGIFSTRILESLGIER
                     LRDDLDALTATSPAEAERLAAALGALRENVREVVNQALLLYGREESENLRHEILRNAP
                     LARLERRQVEQMKALIRAIARRLRESYSKPRKRQRRGHLDIRRTLRRNAAWGGVPFLT
                     SWKRRHRDRPKIVAICDVSGSVAQVSDFFLLLIYSLHEVVDDVRSFAFSGHLIEVSDI
                     LDTKSPEAAMSEIMSKVGFGSSDYGGSLLDFEKGWIRTVTPKTTVIVLGDARTNNLDP
                     RADILRRISERSKRLVWLNPEGRMAWGWGDSEMPRYAAFCSVVRQCATAKQLERAVSD
                     IVAAYQ"
     gene            complement(8067..9014)
                     /locus_tag="VGA15_25455"
     CDS             complement(8067..9014)
                     /locus_tag="VGA15_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9591073.1"
                     /translation="MNNNLTADSIEAVTSGLASAGYIASRQIATAVYLAERIEKPILV
                     EGPAGVGKTELAKALAAWRGLKMIRMQCYEGLDEAKALYEWKYAKQLLYTQILKDKLG
                     EVLGGAETLAGALDRLHDFGDVFFSKEFVEPRPLLQALEQPAGCVLLVDEIDKSDAEF
                     ESLLLEILSDYQVSIPELGTVVALVKPTVILTSNGERDLSDALKRRCLHLHIGFPEQK
                     LEERIVESRVPGISQSLRTQIVGFINQVRTLDLKKLPSVSETIDWARVLVLLQSTELD
                     HETVKDTLNVLLKYEADIETTMPQVTTFVAKAGRQGVFG"
     gene            complement(9014..9535)
                     /locus_tag="VGA15_25460"
     CDS             complement(9014..9535)
                     /locus_tag="VGA15_25460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591074.1"
                     /translation="MQALEVLAPASHCELEFDGCQVPATVRVGDMLDAYPAMALPFRD
                     VEDIVGTANVSGMLSWLLEKSAICIERTEANALRLGRIAGLVSLVHAASRLAVAALDG
                     DGQDIPARTIGVRLLARDLVGEIRDLLAQVQSGDDATSRALAAFDLLSSVARQPRKVR
                     QIRLGNSVWSEKQ"
     gene            complement(9638..>9733)
                     /locus_tag="VGA15_25465"
     CDS             complement(9638..>9733)
                     /locus_tag="VGA15_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045861.1"
                     /note="catalyzes the reaction of formaldehyde with
                     glutathione to form S-hydroxymethylglutathione; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde-activating
                     protein"
                     /protein_id="HEX9591075.1"
                     /translation="RLRELGLEPYDCLSPALMDAIATHVAKSKAA"
BASE COUNT         1895 a         3035 c         2954 g         1849 t
ORIGIN      
        1 tcgacatgcc gttcgagatc gccaatatcc aatgcgagaa tccggaagcc gccgcgcata
       61 cccgcatcgg ctggttccgc tcggtatcga acatcccgcg cgcttttgcg gtgcaatcga
      121 tggtgggcga actggcgcac gccaccaacc gcgaccaaaa ggatatgctg ctggagctga
      181 tcggctctcc gcggatcgtc aagctcgact ccgtgaagga cctgtggaac tacggcgagc
      241 cctatgacag ctatccgatc gataccgcgc ggctccgccg cgtggtcgag gtggtcgcgg
      301 acaagggcgg ctggggacgg accgtgccca aaggacatgg gcttggcatt gccgtgcatc
      361 gcagcttcgt cagctatatc gcgaccatgg tcgaggtatc ggttggcgac aagggcaaac
      421 tgaccgttca ccaggtcgac accgccatcg actgcgggat gttcgtcaac cccgaaagga
      481 tccagtcgca aatcgagggc gcggcgatca tgggcctgag cctcgccaaa tatggcgaga
      541 tcaccttcaa ggacggcaag gtgcaacagg gcaattttga cgacttccct gtggtccgaa
      601 tcgacgaggc acccctcgtg accaacgtgc acatcgtgcc cgccaatgcc gatacgccac
      661 caagcggcgt cggggaaccc ggggtgcctc ccttcgctcc ggccttgatc aacgccatct
      721 tcgcagcgac cgggaaacgc atccgggcac tgccaatcgg aaagcagctt gaaacttaag
      781 acgcgttcga ccgttttagg ctcttggaga ggagcccaca aaaatcacgg cgctcgcggc
      841 actagtctcg agcgtccttc ttgcgaacgt tcaggaggcg gcggcccagg caaacccgcc
      901 gcccagcgcg acagcaacac gaccggcgac gacatcgccg gcgcaaactt ccctgccaaa
      961 cgccagcccg ccggccacaa caactcagac aaccggcgag gccaaccagg atccgactgt
     1021 caaaaaaatg aacgaagacg aaaagcagaa agtcgacacc aagggcaaat agcctgaacg
     1081 ttgattgcgt gagcagttca catttggaac gtcgtcaccg ttcccatgaa gatgatagaa
     1141 aaaagggccc cgcatttcgc gaggcctttt tttatcttta tttgcccata ccggagcctg
     1201 ggcccgtagg agtcagcgtc gatccgcttg gcgaagcgct gggaccgagt gtattgccgg
     1261 atgggctctg ggtgctgttt ggcctggccg gaccccgggc cgacgaacca gccgtgcttc
     1321 cggtagttcc agtggtggac gagccagtgg tagtgccggt tccggtggca gagcttccgc
     1381 cagccgagcc agtgccgctc tgtgcaagtg cgaaagtacc ggaaaccgcg aacgccgcag
     1441 ccagtgtgat cgtcgcaagt ctcttgatcg attccttggc gttaattgac caaggataac
     1501 ccgccggcag cgtcttatgt tccgcggatc ttcagcgcga tgcaagcggc tcgcgcaagc
     1561 gtgttacgcg gcggaacttg tcccccatcg cgtgctactc gcgtccgaag atacgtcaga
     1621 catcgccggg tcacttgagc cagtggtgtt gcattctctg aagagggaag cgatctcggc
     1681 gtgagcgaga tggtcccacc gctccgcgtc gcccaaatgt ttccagctat tttccgggtc
     1741 aaacaccgcg cgttgacggc agatggaact catggcgcga tagcttcgca cttttttcat
     1801 tgccgcctcc cctggacttc aatacgcgcg agcttattcc cactcttcgc gtcagagtca
     1861 ttacgattat atgtcagaac taattttgag atttggtcaa tggacctgac aaatggacat
     1921 ctggtgactc tttcttcaga gcccattggc ctgcccgatt ctgcttggcg ctaagtgtat
     1981 aggtacaagg ccattagcaa aagacttatg tggaacgaaa tcagatgaac ccgcagcttg
     2041 ttggacgaac acggaggctt ggagagctgg gattgaaaaa tccgggctgt gttagccagg
     2101 tcacattcct ccatccaaat cagatttatc ctggcgtggt atggccgcaa acaaggtaac
     2161 ggcggcacac acgcttggct cctcccgcca aggaacgact tagagccgtc agggtgggaa
     2221 ctcggcggct cgattttctt tccgccgtgc cgccatccag gtctcatcgt gtcgacatgg
     2281 tcgttgggcc aaggctgtcg tgcagaccgc gtcgctcttg gccatcaaag tcaggttgat
     2341 tgggttctca atcgggtttc ccgcttggtg cccgacgcca catcgaagga cgcgccttca
     2401 ccgggtccag cagcgaccag tcgccctgct ccagggcgta tccttctgga aacggtggcc
     2461 gcagctccag ccccagggaa cgcaggacac gatcgtcgcg ataatagcat tgcagcacga
     2521 cgcgggtgag cgttgccgcg gcagctccgc ccgatgcacg gaattgccgg gctaccgcct
     2581 cgcgcacggt ctcgccaaga tttgcaagcg gcatcgcggc caggcgcgcg agatgatcga
     2641 gcgcttcgcg caccaattcg gcgtcgcggt cgagcgtggc gaggatgtcg gcctgaatcg
     2701 ccgcgtcgtc cgcgccgggc acaccgtatt gctcgctggc gggaatgatc atggcggcaa
     2761 cgacacgcaa atcgtcgcgc tggagggagc taagggcttt ttccacgggc atgacggcct
     2821 caatcgaaca gattggcaag gcgctgtttc atctgatcgg cgacatagag cgcaatggcc
     2881 tgaatggtgg atgtcggatt taccccacct gacgtgacaa acacactgcc atcgacgata
     2941 aacaggtttt tcacatcgtg tgatcgcccc cattcgttca ccaccgaccg ttcggggtct
     3001 acgcccatcc gcgccgtccc gagcagatgc cagccgccat acaggatcgg gcaattgacg
     3061 cagatatcgg tcgccccggc gacggcaaga atttccttgg cccgcgcaat gccgtgttcg
     3121 agcattcgcg cactgttctc gccgatcgta tagtcgacac gcggcgccgg gatgccatgg
     3181 ctgtccttga ggatgggatc gagcgtgacg cggttatgct cctccggcag gtcctcgcag
     3241 atcgccgaca ggccgagccg gtggccatta agccggcgga acgcggcgtg gtggcccgcc
     3301 ccccacggca acaatccctt ggcctcgctg gtgacggcct ccgtcaccgg tccgatgccg
     3361 cggccgaatt gaaaggcgta gccacgcacg aaaccgcgcg aaggatcggt ttcgtagaac
     3421 tctttgctcc agagacaggt cggcggcgcg cggttgctgt cggtcggctc gtccacgtag
     3481 ccgtaggctt gcgcataggg atggaacatc aggtttttgc cgaccagtcc gctggaattg
     3541 gcaagcccgt tcggaaagcg gccggaggcc gaattcaaga gcagcctcgg cgtgccgacg
     3601 ccgttacagg cgatcacaac gacttcggcg ggccgaaatt gctcgacgcc atccttgtcg
     3661 taatacacga cgccggttgc catgccctgc ccattggtgg tgatctcgcg cacccggcaa
     3721 tgcgtgcgaa gctcgacgcc ggcgcgcaac gccacaggcc agtaggtgat gtcggtgctc
     3781 gcctttgcgc cctgcgcgca tgccggcgtg caatggccga gattgatgca tcgtgcgcgc
     3841 ccctcgtaat cgacggttgc caccgtggta tcggacggcc accagtgcca gccaagcttg
     3901 ttcatggctt tcgcaagacg ggtcccggag cggccaagcg gcagcggcgg catcggagga
     3961 tcggtgggtg gaaaggccgg atcgccggcg aggccggaaa cgcccatcat ccgatcgttc
     4021 tcggcaaaga acggcgccag cgtgtcgtaa tcgatcggcc agtcctcggc cacgccatcc
     4081 agcgtgcgca cgcgaaagtc cgaagggtga agtcgcggga aatgcgccgt atacatgatg
     4141 gtagagccgc caacgccgtt gaagttcacc accttgatcg gcgagttgtc gtcgttgacg
     4201 ggataatcct ccggccgcgc gcgaatgttg ggactgattg aataatcgcc ataggcgcgc
     4261 gcctcccagt cccgccccac gcttggatat tgggccggat tcatccagtc gccttgttcg
     4321 aggcacagga tgtgcatctt ggtttcggca agtgaccacg cgacagcggc gcccgacgca
     4381 ccggcgccga tgatcaacac gtcaacggga tcgttcattc cggttccttc tgtcattccg
     4441 ggatggtccg aaggaccaga cctcagatgt gcaattgcac atcggggaat ctcgagattc
     4501 cgggttcgat gcggagcctg tcatcgggcc ggccaaaggc cggacccgtt ggcatcgccc
     4561 cgcaatgacg acccataacc tcaattcacg atccgcaccg gtaacgaccg tatgccgcga
     4621 atgaaattcg aatagagccg ctgcggcggc cccatgatct cgaagcgaag gtcccgctgc
     4681 aggatttcct cccagagaat ccgcagttgc tgctcggcga gccggtcgcc gacgcagcga
     4741 tggatgccgg cgccgaacgc gagatgatgc cgtggttttg cgcgatcgac gatgaattga
     4801 tcggcgtgct cgatcttttc ctcgtcgcga ttgccggaga tgtaccacat caccaccttg
     4861 tcgcccttgg cgatgctgcg gccggcgagt tcggcgtcgt tgcgcgcggt gcggcgcatg
     4921 tgcagcaccg gcgtctgata gcggatgatt tcggaaacca gattcggaat caaatcgcgc
     4981 cggctgcgca ccagttcgaa ctgttcgggg ttttcgacca gcgccatcag tccgccgctc
     5041 atggaattgc gcgtggtgtc gttaccgccc acgatcagca ggccgagcgt gccgataaat
     5101 tcgcgcggcg gcatattgcg ggtcgcgtcg gaatgggcga gcatcgaaac caggtcgaac
     5161 gctggcggcg cggccgcgcg cgcatcccac aatttgcgga aatatgctcc catcttgtcc
     5221 agttcggcga aacgctcatc ctccgaatgc acgacggcat cgggcgaatc cacattggcg
     5281 atcgcgacat cggaccacca ggtcagcctc tcacggtccg cccatgggaa atcgaacagc
     5341 gtcgtcagca tcatgttggt caggtcgacc gaaacccgcg tcacccagtc gaaggtctcg
     5401 tgacgcggca gcgcgtcgag cacatcggcg gtacgcttgc gaatcaacgg ctcgagattg
     5461 acgaggttgg atggtgccac gatcggcgcg acggtgcggc gctgcgcggt gtgccccggc
     5521 gggtccattc ggataaagct cgcgcgctcc tgtcccttcg gctgatccgc gacctggatg
     5581 ccgccgagct ccgaactcga cgagtagttc tcatggtcga gctcgaccgt gaagatgtcg
     5641 tcgtagcgcg tcaccgacca atacggtccg aaccgcgacg cctcgcagta atgcacggga
     5701 tcgtcgcggc gcaacgtcgc aaacagcggc cgccaggcgt cttcctgata aagcttgggg
     5761 ttgctgacgt cgatcgatgg gagcgcgcgt tgccgcgctt ctgcctggac catggtttcg
     5821 ctcccgtacc gttgccgtct atccggcgat ggagcatagg acgggatcag gtgctggcaa
     5881 aggggaggca agttcgccaa cggttgtccg ctttgacgtc aaggtcggcc atgccgcaag
     5941 ggtcgtgagc gtttgggttg cacgctgcag ccctgggttg atccacggcg gcggcgcgac
     6001 ctatccagct ggatatcccg caccgatcga tctgcgccca atccagccac agatcgccac
     6061 tatttcgccg gcgcgacacc tcaagaggtt cgcactgaca cgatttagtc acgggcgggc
     6121 gttcagggtc aactgtggcg agcagacaac cctcgccgcc tcaagcgata tcaacataat
     6181 ttagggcgca atgagacgta cgatgaagac agaatcatca tccgcgcgtt aagttcgagc
     6241 cgagtcgagg gctggattcg gctcgactgc ggcactgatg taatccacga ctacacgatg
     6301 ggccgcgaag cacgactcat cagttcctgg atcgcatata cgttcaatgt ggaataacat
     6361 gacgcacgct tcgctactca acccgttcag ttcgagccaa gagccatccc aaatcgccaa
     6421 gcggcgggtg gcacgggcga cgcggctgac acctgtctgc tccaattgtc actcagacga
     6481 catcatctct caggcgacgg cccagtggag caacgaatca caggaatggg agctggctga
     6541 caccttcagc caaccggcac actgcacccg ctgcaacggc ccgtgcggca taacctggct
     6601 accgctcaac tgaacctgtc aggcgacgct acctcgcggc tacctcgcgc cctggcaata
     6661 tcctgccggg gcttttcgtg aacccggtct aacgaatcac tggtaggcgg ccacgatgtc
     6721 cgagacggcc cgttcgagct gctttgccgt cgcgcactga cgcacgacac tgcagaatgc
     6781 cgcatatcgt ggcatttcgg aatcgcccca gccccaggcc atgcgtcctt cggggttgag
     6841 ccagaccagc cgcttggatc gctctgaaat gcggcgaagg atgtcggcgc ggggatcgag
     6901 gttattggtg cgcgcatcgc caagaacgat caccgtcgtc tttggtgtca ccgtccggat
     6961 ccagcccttc tcgaaatcca gcaatgaacc gccatagtcg gacgagccaa atccgacctt
     7021 cgacatgatc tcgctcatgg ccgcttcagg cgatttggtg tcgaggatat cgctaacctc
     7081 gatgagatgg ccggagaagg caaacgagcg cacgtcgtcc accacctcat gcaggctata
     7141 aatcaacagc aggaagaagt cggacacctg cgccaccgat cccgagacat cgcagatcgc
     7201 cacgatcttc ggccggtcgc gatgccggcg cttccatgag gtgaggaacg ggacgccgcc
     7261 ccaggcggcg ttgcgccgaa gcgtccgcct gatgtcgaga tgcccgcgcc gctggcgttt
     7321 gcggggtttg ctgtagctct cgcgcagccg tcgtgcgata gctcggatca gggccttcat
     7381 ctgctcgacc tgccggcgtt cgaggcgggc gagcggcgcg ttgcggagga tttcatggcg
     7441 gagattctcg gattcctcgc gcccgtaaag aagtagcgct tgattgacga cctcccgcac
     7501 gttttcgcgc agcgccccca atgcggccgc gagccgttcg gcctccgccg gactggtagc
     7561 cgtcagcgcg tccaaatcgt cccgaagacg ttcgatgcca agggactcga gaatccgtgt
     7621 cgagaagatc ccgcgctggg taaaataccg gatgtcggaa agcgaggcgg cattcgccgc
     7681 attcgccatc gcggccgcga tcgcgttgcg gtcccgcgac agcagcatct gcgcgagttc
     7741 gccgagttcc ggcgatggcg cgccggcgtc gcccgatggg gtctcgccag ctgccgcatc
     7801 ccgctgcgtg ctttcggccg catcctccgg cgcggtctcc tcctccttca cctccggctg
     7861 gcggaagaaa aggtcaaaac attcgccaag cgcctgcttt tcgtcctggc tcttggcgag
     7921 cgtcaacagc agcgtgtcgc gcagaatgcc tctgtcgcca aatccgacat cggccactgc
     7981 cttcatggaa tcgatgcttt ccgcgggcga cacccgaacg ccggcgccgc gcgccgcccg
     8041 gaagaaacga tgcagttctt ctctcatcag ccgaacacgc cttgccggcc cgccttggcg
     8101 acgaaggtcg tcacctgggg catcgtggtc tcgatgtcgg cctcgtattt caagagaaca
     8161 ttgagcgtgt ccttgacggt ctcatgatcc agttccgtcg actgcagcag caccaggacg
     8221 cgcgcccaat cgatcgtctc gctgaccgag ggcagcttct tcagatcaag ggtgcggacc
     8281 tggttgatga agccgacaat ctgcgtgcgc agggattgcg agattccggg gacgcggctt
     8341 tcgacgatgc gttcttcgag cttctgctcg gggaagccga tatgcagatg caggcacctg
     8401 cgcttgagcg catcagagag gtcgcgttcg ccattggatg tcaatatcac cgtcggcttc
     8461 accagcgcca cgacggttcc gagttcggga atgctgacct ggtaatccga caggatttcg
     8521 agcagaaggg attcgaattc ggcgtccgac ttgtcgatct cgtcgaccag cagcacgcag
     8581 cccgcaggct gctccagggc ctgcagcaga gggcgcggct ccacgaattc cttcgagaag
     8641 aatacgtctc cgaaatcgtg caaccggtcg agtgctccgg cgagcgtttc ggcgccgccg
     8701 agcacttcgc cgagcttgtc cttcaggatc tgggtgtaga gcagttgctt ggcgtacttc
     8761 cattcgtaca gcgccttggc ttcatcgagc ccttcgtagc attgcatgcg gatcatcttg
     8821 agcccgcgcc atgccgcgag cgccttggca agctcggtct tgccgacgcc ggccggacct
     8881 tcgaccagga tcggcttctc gatacgctcg gccagataga ccgcggtcgc gatttggcgg
     8941 ctggcaatat agcccgccga ggccagtcct gatgtgacgg cctcgatcga atccgcggtt
     9001 aaattgttgt tcattattgt ttctcgctcc ataccgaatt gccaagccgg atctgccgca
     9061 ccttgcgtgg ctggcgtgcg accgaggaca aaagatcgaa ggcggcgagc gcccgggagg
     9121 tcgcatcgtc acccgattga acctgtgcaa gcaggtcgcg gatttctccg acgaggtcgc
     9181 gcgccagtag ccgcacgccg atcgtccgcg ctggaatgtc ctgcccgtcg ccatcgagcg
     9241 ctgccaccgc gagccggctc gccgcgtgca cgagcgatac cagacccgcg atgcggccga
     9301 gccgtaaagc attcgcttcc gtgcgctcga tgcatatggc ggacttttcg agtagccagc
     9361 tcagcatccc cgaaacgtta gccgtcccga caatgtcctc gacatcgcga aacggcaagg
     9421 ccatggccgg ataagcgtca agcatgtctc cgacccgcac cgtcgcaggc acctgacatc
     9481 cgtcgaactc cagctcgcag tgcgacgccg gcgcgagcac ttcgagggct tgcatcggct
     9541 ttaccgtcag gccgggtgtg ccgcgtgtca aaagtgatcg taacctcgct gacggcggag
     9601 atcgccgcca gcgaaaagct tgcgcaaggc tgccggatca ggccgctttg gatttcgcca
     9661 catgggtggc gatcgcatcc atcagggccg gcgacaggca atcatagggc tcaagtccga
     9721 gttccctgag gcg
//
LOCUS       DASWGM010000943         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_132104,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000943 DASWGM010000000
VERSION     DASWGM010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132104"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VGA15_25470"
     CDS             complement(<1..1568)
                     /locus_tag="VGA15_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9591076.1"
                     /translation="MASISEPFIRRPVGTTLLAIGLFLVGGVAYQFLPVAAVPNVDFP
                     SIRVSATRPGADPSVMAATVAAPLERRLGEIAGIDQITSTSSLGNTSIQLQFAIGRDI
                     DRAARDVQAAINASLADLPSDLPSLPRFRKANSAAAPVFVLALTSKTISSSRIYDVAD
                     TVLAQRISQVPGVGEVTVSGADQPAVRIALNPVALSNAGIATDDVRLAIINANPLGPV
                     GIFNGGRQSETLSINRQMRTAAEFRDIVIKSANGNFVRLSDVAEVEDSVRNSRSIAWF
                     NKQPAVLIQITKQGDANVIDTVDRVRALIPELKQWIPAGVDISTLVDRTGTIRASVAD
                     MQLTLLATAILVMVVVFAFLRRITPTIAAGVSVPLALAGTCAGMWLAGFSIDNLSLMA
                     LAISVGFVVDDAIVMIENMYRNLEHGMRPYQAALEGAKQIGFTVLSISLSLIAAFTPL
                     IFMDGIVGRLLREFSLTLTFAIVVSTVVSLTITPMICAHYIKQATSHNATWFDRVVEG
                     SLSRIVAFYARTLRV"
     gene            complement(1568..>3538)
                     /locus_tag="VGA15_25475"
     CDS             complement(1568..>3538)
                     /locus_tag="VGA15_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9591077.1"
                     /translation="LSLMALTIGTGFVVDDAIVMIENIVRHMENGETAMEASLRGASE
                     IGFTVISLTVSLIAVFIPLLFMSGLVGRMFREFALTLTIAVVTSAIVSLTLTPMMCSR
                     LLKHAGEEVAVPGLAAISRGIDRMIEFYHRTLLWVLERQRATLLVTFATLAATLILYV
                     VAPKGFLPLQDTASITAVTQAGPDVSFAEMQSRQTQAADAIKADPDVTGVVSVIGAGS
                     VNPTTNVGRLVMTLKPRGERRDDVSVVVARLKEKVASIPGMTIYFQPVQDVQISTQSS
                     RSQYQYTLTGTDSAQVSLWANNLVAELRRDPLFRDVSSEAQDGGLRAELDINRQRAGQ
                     LGVSLQAVTDTLNDAFAQRQISTIYGQANQYRVVLEALPMYQRDPSILSKLYLPGALD
                     AQVPLSAVATLTRTTAPLAISHQAQFPAVSLSFNLAPGAALGDAVDAVKSIETRIGMP
                     GSIVGVFAGDAAEFAKSLAGQPWLILAALVTIYIVLGVLYESYIHPITILSTLPSAGV
                     GAILALMLFGQDLSVIGLIGIILLMGIVKKNAIMMIDFALEAERHQGMSANDAIVQAC
                     LLRFRPIMMTTLAALFGALPLAVESGTGAELRFPLGISIIGGLLLSQLLTLYTTPVIY
                     LALDRLNRRIEKAVPPAGPQLPPVAGATEGMQ"
BASE COUNT          687 a         1114 c         1148 g          589 t
ORIGIN      
        1 acccgcagcg tccgcgcata gaatgccacg atgcgcgaca acgagccttc gacgacacgg
       61 tcgaaccagg tcgcattgtg ggaagtggct tgcttgatgt agtgggcgca gatcatcggc
      121 gtgatcgtca gcgacaccac ggtcgaaacc acgatcgcaa atgtcagggt cagcgaaaat
      181 tcccgcagca gccggccgac gatgccatcc atgaagatca gcggggtgaa ggctgcgatc
      241 agcgacaggc tgatcgacag taccgtgaag ccgatctgct tggcgccttc gagggcggcc
      301 tgatatggcc gcatgccatg ctcgaggttg cggtacatgt tctcgatcat cacgatggcg
      361 tcgtcgacca cgaagccgac cgagatcgcc agcgccatca gcgacagatt gtcgatcgag
      421 aaaccggcga gccacatccc ggcgcaggtg ccggccaagg ccagcggcac cgagacgcct
      481 gccgcgatcg tcggcgtgat ccggcgcagg aatgcgaaca ccaccaccat gaccaggatg
      541 gcggtcgcca gcagcgtcag ctgcatgtcc gcaacgctgg cgcggatggt tccggtgcgg
      601 tcgaccagcg tcgaaatgtc gaccccggcc ggaatccatt gcttcagttc gggtatcagc
      661 gcgcgcacgc gatcgacggt atcgatgacg ttggcgtcgc cctgcttggt gatctggatc
      721 agcaccgccg gctgcttgtt gaaccaggcg atcgaccggc tgttgcggac ggagtcttcg
      781 acctcggcga cgtcagagag ccggacgaaa ttgccgttcg cactcttgat cacgatatcg
      841 cggaattcgg ccgcggttcg catctgcctg ttgatcgaca aggtttcgct ctggcggccg
      901 ccattgaaga tgccgaccgg gcccagcgga ttggcattga tgatggcgag ccggacatcg
      961 tctgtggcga tccccgcgtt cgacagcgcc accgggttga gcgcaatgcg caccgccggc
     1021 tggtcggcgc cgctgacggt gacctcgccg acgccgggca cctgcgagat gcgctgcgcc
     1081 agcacggtat cggcgacgtc atagatcctg ctggaggaaa tcgtcttcga cgtcagcgcc
     1141 agcacgaaga ccggcgccgc cgcggaattg gctttgcgga agcgcggcaa cgacggtaag
     1201 tcgctcggca ggtcggcgag cgaagcattg atcgcggcct ggacatcgcg cgcggcgcgg
     1261 tcgatgtcgc ggccgatggc gaattgcagc tgaatgctgg tattgccgag cgaactggtc
     1321 gaggtgatct ggtcgatgcc ggcgatttcg ccaagccttc gttccaaggg tgcggcaacg
     1381 gttgccgcca tcaccgatgg atcggcgccc ggccgggtcg cggaaacccg gatcgacgga
     1441 aagtcgacat tgggaaccgc ggccacgggc aaaaactggt aggccacccc gccgaccaga
     1501 aaaagcccga tcgccagcag tgtggtgccg accggccgcc gaatgaaggg ctccgagatc
     1561 gatgccatta ctgcatcccc tcggtggctc cggcgaccgg cggcagttgc ggtcctgcgg
     1621 gcggcaccgc cttctcgatc cggcggttga gccggtcgag cgcgaggtag atcaccggcg
     1681 tcgtgtagag cgtcagcaac tggctcagca gcagtccgcc gatgatcgaa atgcccagcg
     1741 gaaaccgcaa ctcggcgccg gtgccgcttt ccaccgccaa cggcagcgcg ccgaacagag
     1801 ccgccagcgt cgtcatcatg atcgggcgga accgcaacag acaggcctgc acgatcgcgt
     1861 cgttcgccga catgccctgg tggcgctcgg cttccagcgc gaagtcgatc atcatgatcg
     1921 cgttcttctt gacgatgccc atcaacagaa taattccgat caggccgatc accgacagat
     1981 cctgtccgaa cagcatcagc gcgagaatgg cgccgacacc cgccgacggc agcgtcgata
     2041 aaatggtgat cgggtgaata tagctttcat agagcacgcc gagcacgatg tagatggtga
     2101 cgagggctgc caggatcagc cacggttgtc ccgccagcga ttttgcgaac tcggccgcat
     2161 cgccggcaaa cacgccgacg atgctgccgg gcatgccgat gcgggtttcg atcgatttga
     2221 ccgcgtcgac ggcgtcgccg agcgcggcac ccggcgccag attgaagctg agcgacaccg
     2281 ccggaaattg cgcctggtgc gaaatcgcca gcggtgcggt ggtgcgcgtc agcgtcgcga
     2341 ccgccgacag cggcacctgg gcgtcgagcg cgcccggcag atagagtttt gagagtatcg
     2401 acggatcacg ctgatacatc ggcagcgcct ccagcacgac gcgatactgg ttggcctgcc
     2461 cgtagatggt cgagatctgc cgctgtgcga aggcatcatt cagcgtgtcg gtcaccgcct
     2521 gcaggctgac gccgagctgg ccggcgcgct ggcgattgat gtcgagctcc gcgcgcaatc
     2581 ccccgtcctg cgcttccgaa gagacgtcgc gaaacagggg atcgcggcgc agttcggcga
     2641 ccaggttgtt ggcccagagc gaaacctgcg ccgagtcggt gccggtcagc gtgtactgat
     2701 attgcgaccg gctggactgg gtcgagatct gcacatcctg taccggctga aaatagatcg
     2761 tcatgcccgg gatcgacgct actttttcct tcagccgcgc gaccacgacg gaaacgtcgt
     2821 cgcgcctttc gccgcgtggt ttcaacgtca tcaccaggcg gccgacattg gtggtcggat
     2881 tgaccgagcc ggcgccgatc accgagacga cgccggtcac gtcaggatcg gccttgatgg
     2941 catcggcggc ctgggtctgc cggctttgca tttccgcaaa ggaaacgtca ggaccggcct
     3001 gggtcaccgc ggtgatcgag gccgtgtcct gcaacggcag gaagcccttg ggcgccacca
     3061 catacaggat cagcgtcgcg gccagcgtgg cgaaggtgac cagcaaggtc gcgcgctggc
     3121 gctcgagcac ccacaacagc gtgcgatgat agaactcgat catgcggtcg atgccgcggc
     3181 tgatcgcggc aaggcccggg accgcaactt cctcgccggc gtgcttcaac agccgcgagc
     3241 acatcatcgg cgtcagcgtc agcgacacga tcgcggaggt cacgaccgcg atcgtcaagg
     3301 tcagcgcgaa ttcacggaac atgcgcccga ccagtccgga catgaacaat agcgggatga
     3361 agaccgcgat cagcgacacc gtcagcgaaa tcaccgtaaa gccgatttcg ctggcgcccc
     3421 ggagcgaggc ttccatcgcc gtttcgccat tctccatgtg acggacgatg ttttcgatca
     3481 tcacgatggc gtcgtcgacc acgaatccgg tcccgatcgt caacgccatc agcgacag
//
LOCUS       DASWGM010000944         5519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2727932,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000944 DASWGM010000000
VERSION     DASWGM010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2727932"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1662..2174
                     /locus_tag="VGA15_25480"
     CDS             1662..2174
                     /locus_tag="VGA15_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX9591078.1"
                     /translation="MIVFSRSFIRKRVISLATTASVAATSLALAHGPTRAYHVRGTMP
                     IQYVADRPDYSDEQPFLSENDAAMNKMMADMTAKPTGDVDRDFVAMMVPHHQGAVDMA
                     KAELRYGHNEQLRRLAQEIVVTQQQEIAVMQRALGDKSAASPTKPAAVSSPPTMSHET
                     MGMSGDAAHH"
     gene            2216..2728
                     /locus_tag="VGA15_25485"
     CDS             2216..2728
                     /locus_tag="VGA15_25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX9591079.1"
                     /translation="MISVSHASISLRALLATVAMVGVATFVVARQSQPPFVISPLTLC
                     STTTQAAFPDETPYLTENDAAINKMMSDMVIKPTGDVDRDFVAMMTPHHQGAIEMAQA
                     VLRSGRNEQIRRLAQEVIVTQQQEIAAMRMAIGDPLPPSAPSPTQISATPTSRVPSIL
                     TSFSKTGELQ"
     gene            2725..4215
                     /locus_tag="VGA15_25490"
     CDS             2725..4215
                     /locus_tag="VGA15_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX9591080.1"
                     /translation="MRRGLLTFGFLASVCFAGQTAAWAGQAPGAASDPDIPVSHHDRV
                     YSAEQFSNTVSVTDPVDNKLVGVIRLGDPQPGNFSPLYKGQVLVHGMGFSPDHKTIAV
                     VSIGSNSVTFIDTATNAVKHTTCVGRSPHEVFFTPDGKEVWVTVRGEDYVAVLDGQNF
                     EEKSRIKVPAGSGMQIFSPDGKYGYVCSSFNPETVVITVADHQIVGRVKQDSPFCPNI
                     AATPDGKQVWFTLKDIGRTMVFDAQPPFAVLKSIDTGPITNHVNFARNASGTFAYVTI
                     GGLNEVKVFRTNDFSLAATIPVGKLPHGVWPSGDGSRIYVGLENADSMAAIDTATNKL
                     IATSPIGQAPQAITYVPNAVPEGDGTQGLQQLGVAGQTAHLSLGAVEGRKTVGSAGGA
                     GSEDKAPTSVSLFDQGLIQVLQASVTGLEPKQPYVLALAHDRNGEGPLEPLAAFMTNP
                     AGSAIVNATGAIRQVVRGEDKIQRRYLVIADGHADKPGPIGQIQLK"
     gene            4269..>5519
                     /locus_tag="VGA15_25495"
     CDS             4269..>5519
                     /locus_tag="VGA15_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9591081.1"
                     /translation="MGIVRFALRFPHTFYVVAALILFLGIAAIRSMPTDIFPEIRIPV
                     VTVIWQYTGLTTPEMEQRVSTYSQYSISANVNGIKNMEAQTLNGLSIQKIYFQPDVNL
                     DLAIAQIVSATNAIRALMPPGIQPPIVVQFNASSVPVLQLSLNSDSLNEQQLYDFGIY
                     RVRQELAPVPGVTLPTPAGGKYRQIMVDIDPDKLLSRGLTPLDIVNAVNTQNLTLPSG
                     TAKIGKTQYTVRTNATPASINDLNMMPVKFVNGATILLKDVAQVRDGSLVQQNIVRED
                     GRRSVLLSVIKNGNASTLAVVNGVKKALQAARAAAPAGLKINELFDQSVFVTNSVTGV
                     LREGAIAAGLTALMILIFLGSWRSTLVVMISIPLAMLSSLVVLYFLGETLNTMTLGGL
                     ALAVGILVDDSTVTIENTHRLWTEE"
BASE COUNT         1117 a         1681 c         1612 g         1109 t
ORIGIN      
        1 cgatgagggg ggtgtggaaa cagggaccat ggctcggccg ctcaggcacc gccagacgaa
       61 aggggcggaa acagatacgc cgggcctaac gttcactgcg ccacactcct actctaccgt
      121 taaaaccatt gcgtgcggga acgccgggtg attccggggt actcgtttac tcgtgtgctt
      181 tttaccaatg caaagcgccc acgaggccgc gggtgcaacg ggcatccggc gttccccacg
      241 ccctctgtct aaaggaggga caaggttcat gcacaactcg ggcgcatcgc gccgcgggat
      301 cgcgcgcaca cgtgttcggc tctttgaaaa ttgaatcaga tgttcgaaac gccgcgccat
      361 ctggcgaaca gattgggccc cttgcggctc cctgtgccgc cgccagcgta ccatccatca
      421 atttggtgag cggcttccat cgcgctaaag aagaatttaa tggtgtcggc gcggttgtct
      481 cttatctaag cagcaaagga gacaaccatg aaacttacga caatcgcact aacaattgca
      541 ttagttctcc caagcacatt cgcgcttgca cgcgggatca gtgttggaag tcacgtcact
      601 cgcctcttcg gaggcaccgc tgtcggtgcg gcaggccgta ttgcaaccaa gcccaggaat
      661 gtttctggga atgcgcttgc tcccatcgcg catgatccaa gcggatcaac actaactccg
      721 tcagctatga accgcggcgg ttagaataaa gaaaaccccg cgaactagcg gggctactgc
      781 ctgagcagtc ttcttgggaa atgtgacctc tctggaaagg tggaattccg atgcgttctg
      841 ttttggcctt aggcctgtta atcaccctgt gtgcttctgc caacgccaca acggtacatc
      901 ggacgttaca cctacgcacg cgcccacatg ttatcgttcc cagccaaggt gtgaccgccc
      961 cgcgcttcgc ggtacccggc tggaccgacg aagcaacccg gcgctggctg gataacgcca
     1021 gcgcgccctg gacacacgcc tgacggtcgc tccccaaccg attgtccgaa ccacgcgccg
     1081 cgggtcggga tagcgggctg gaattgcatg ctggctcgat atccctagag gcctcacagc
     1141 gctagtgcca tttttcttgt tactccttga tcggccgtaa cggccgcgcg ccttcgtgat
     1201 tgcacctcgc ccttcggacg gcgccggagc gtttctgacc aggctcttcg ctcgacaagg
     1261 tgaaaaatag cttccaccgc gttcaacccg gacatcgttg ggtttctcca atttgtcgat
     1321 cgtttgcgag gttcatcgtt ctgaaatcag gacataagtc tttgcgcttt gcctcacact
     1381 tctttcatgg cgctgctggc acgagatcct ggtccgagac agaagtgccg cccggcatgc
     1441 ggatcacgac aacgccgtgg ttcataaatt cgatttgaga attctaaact ggtttttaat
     1501 tgagaagatg gcgttcggcc gtcatgtttg cggggctccc ggcgctttct ctgtgctgcc
     1561 ggaagcttgt ttgggcgaac cttccaagaa aatatcggca aacgcacaag ccggatcgcg
     1621 ggagttcaag accgcgtgac ctgactttgt ccggaggtaa gatgattgtg ttctcgcgct
     1681 cctttattcg caaacgtgtg atctcgcttg cgaccactgc gtcggtcgcg gccacgtcgc
     1741 tggcgttggc tcacgggccg acgagggcgt atcacgtccg cggcactatg ccgatccaat
     1801 acgtcgcgga tcggccagac tattccgacg agcagccatt cttgtcggaa aacgacgccg
     1861 ccatgaacaa gatgatggcc gacatgacag cgaagccaac cggcgatgtc gatcgcgatt
     1921 ttgtggcgat gatggtgccg catcatcagg gcgcggtcga tatggccaag gccgagctgc
     1981 ggtatggcca taacgagcag cttcgccggc tggcgcagga aatcgtcgta acccagcagc
     2041 aggagatcgc cgtgatgcag cgtgcactgg gcgataaatc agcggcgtca ccgaccaagc
     2101 cagcagcggt ttccagtcct ccaacgatgt cgcatgaaac gatggggatg tccggtgatg
     2161 ctgctcacca ttgaaccaga aagattcgat cgttgaaccg ttcggagagc tcgccatgat
     2221 ctctgtctca catgcatcga tttcccttcg cgccttgctg gccacggtcg cgatggtggg
     2281 cgtagcgaca tttgtcgtgg cgcgacaaag tcagcccccg ttcgtgatct ctccgttgac
     2341 gctttgctcg acgacgaccc aggcggcctt tcctgacgaa acgccatatc tgacggaaaa
     2401 tgacgccgcc ataaacaaga tgatgtccga catggtgatc aagccgaccg gtgacgtcga
     2461 ccgcgatttc gttgcgatga tgacgccgca tcatcaaggc gcgatcgaga tggcgcaagc
     2521 cgtgctgcgc agcggccgta acgaacagat caggcggctc gcccaggagg tgatcgtgac
     2581 gcagcagcag gagatcgcgg cgatgcggat ggcgattggg gacccgctgc cgccgtcggc
     2641 tccgtctcca acgcaaatct ccgccacccc gacttccagg gtgccttcga ttctaacgtc
     2701 gtttagtaaa acaggagagc tccaatgagg cgcggccttt tgacgttcgg ttttctcgcg
     2761 agtgtctgtt tcgccggaca aacggcggcc tgggcgggcc aagcgcccgg agcggcgtcg
     2821 gatccggata ttccggtcag ccatcacgac cgggtctatt ccgcggagca gttttccaac
     2881 accgtctcgg tgaccgatcc cgtcgacaac aagctcgtcg gcgtgatccg gcttggcgat
     2941 ccgcagcccg gcaatttcag tccgctctat aagggacagg tgcttgtcca cggcatgggg
     3001 ttctcgccgg accataagac catcgcggtg gtttcgatcg gctcgaactc cgtcaccttc
     3061 atcgataccg cgaccaacgc ggtcaagcac accacctgtg tcggccgctc gccgcatgag
     3121 gtgttcttca cgcccgatgg caaggaggtc tgggtcaccg tccgcggtga ggattacgtc
     3181 gccgtgctcg acggacagaa ttttgaggag aagtcgcgca tcaaggtgcc ggcgggatcg
     3241 ggcatgcaga ttttctcgcc cgacggcaaa tacggttatg tctgctcctc gttcaatccg
     3301 gagaccgtgg tcatcaccgt cgccgatcat cagatcgtcg gccgcgtcaa gcaggacagt
     3361 ccgttctgcc cgaacatcgc cgccacgcct gatggcaagc aggtctggtt cacgctgaaa
     3421 gacatcggca ggaccatggt gttcgacgcg cagccgccgt tcgcggtgct gaaatcgatc
     3481 gacaccgggc cgattaccaa ccacgtcaat ttcgcccgca atgccagcgg cacgttcgcc
     3541 tatgtgacga tcggcggctt gaacgaggtc aaggtgtttc gcaccaacga cttctcgctc
     3601 gctgcgacga taccggtcgg caagctgccg cacggcgtgt ggccgtccgg cgatggcagc
     3661 cggatttacg tcgggctgga aaatgccgac agcatggccg cgatcgacac cgcgaccaac
     3721 aagctgatcg cgaccagtcc gatcggtcag gcgccgcagg cgatcactta cgttccgaat
     3781 gccgtgcccg aaggcgacgg cacgcagggc ctgcaacagc ttggtgtcgc cggacaaacg
     3841 gctcatcttt cgcttggcgc cgtggaaggc cgcaagacgg tcggtagtgc cggtggtgct
     3901 ggtagcgaag acaaagctcc aaccagcgtc tcgctgttcg atcagggcct gatccaggtg
     3961 ctgcaggctt cggtgaccgg actcgagccg aaacagcctt atgttctcgc gcttgcacac
     4021 gaccggaacg gtgaggggcc gttggagccg ctggccgcct tcatgaccaa tccggcgggc
     4081 tcggcgatcg tcaacgccac cggcgcgatc cggcaggtcg tgcgcggcga ggacaaaatt
     4141 cagcggcgct atctagtgat cgcagacggc catgccgaca agcccggtcc gatcgggcag
     4201 atacagctga agtagtaaac accgccgatt gcgcaatttg ggaaaaccgg tagcaaaaag
     4261 gatagatgat gggcattgtt cgtttcgccc tgcgatttcc gcacacgttc tatgtggtgg
     4321 ccgccctgat cctgtttctg ggcatcgccg ccatccgatc gatgccgact gatatttttc
     4381 cggagatccg cattccagtg gtcaccgtga tatggcaata caccggcctc accacgccgg
     4441 aaatggaaca gcgcgtcagc acctacagcc aatattccat cagcgccaac gtcaacggca
     4501 tcaagaacat ggaagcgcag actctcaatg gtctgtcgat ccagaagatc tacttccagc
     4561 cggacgtcaa tctcgacctc gctatcgcgc aaatcgtctc cgctaccaat gccattcgcg
     4621 cgctgatgcc gcccgggatc cagcctccga tcgtcgtgca gttcaacgcg tcgagcgttc
     4681 cggtcctgca gctcagtctt aattcggaca gcctgaatga gcagcagctt tacgacttcg
     4741 gcatctaccg cgtccgccag gagttggcgc cggttcccgg cgttaccctg ccgaccccgg
     4801 ccggcggcaa gtatcgtcag atcatggtgg atatcgaccc ggacaagctt ctgtcccggg
     4861 gactgacgcc gctcgatatc gtgaatgcgg tcaatactca gaacctgaca ctgccgtcgg
     4921 gaacggcgaa gatcggcaaa acccaatata cggtgcgaac caatgcgacg ccggcgtcga
     4981 tcaacgatct caacatgatg ccggtcaagt tcgtcaacgg cgccaccatc cttttgaagg
     5041 acgtggcgca ggttcgcgac ggatcgctgg tccagcagaa catcgtgcgc gaggatggcc
     5101 gccgatcggt gttgctcagc gtgatcaaga acggcaacgc ctccacgctg gcggtcgtca
     5161 acggcgtgaa gaaggccctg caggcggctc gcgccgcggc gccggccgga ctcaagatca
     5221 acgagctgtt cgaccagtcg gtattcgtga caaactctgt caccggcgta ctgcgggaag
     5281 gcgccatcgc ggcaggcctc accgcactga tgatcctgat cttcctcggt tcctggcgct
     5341 cgacgctggt ggtgatgatt tcaatcccgc tggcgatgct gtcgtcgctc gtcgtgctct
     5401 acttcctcgg cgaaacgctg aacaccatga cgctcggcgg cctcgcgctg gcggtcggca
     5461 tattggtcga cgattcgacc gtgaccatcg agaacaccca tcgtctatgg accgaagaa
//
LOCUS       DASWGM010000945         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2203567,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000945 DASWGM010000000
VERSION     DASWGM010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203567"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..174
                     /gene="ykgO"
                     /locus_tag="VGA15_25500"
     CDS             49..174
                     /gene="ykgO"
                     /locus_tag="VGA15_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEX9591082.1"
                     /translation="MKVRNSLKSLRGRHRNNRLVRRKGRVYVINKVQRRFKARQG"
     gene            332..928
                     /locus_tag="VGA15_25505"
     CDS             332..928
                     /locus_tag="VGA15_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9591083.1"
                     /translation="MIAAAPALAFAQDPGATPPKPQKKLPEAPSKLPKVGADRTRGLD
                     FLFGALKAAPDEASAKHVEARIWALWMQTPSDTAALLMMRARAAMDAQQMDVALKLLD
                     AVVKLRPDYVEGWNRRATLYYLKNDYTHSLEDIEQVLVREPRHFGALAGLGMIMQDIG
                     DDKRALDAFRKALAVNPHLEKVPELVKTLSEKVEGRDI"
     gene            1050..1292
                     /locus_tag="VGA15_25510"
     CDS             1050..1292
                     /locus_tag="VGA15_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591084.1"
                     /translation="MKSLLLAGAMMFAITTVCFAEELYWVVGNRATNRCEIVTSNPVV
                     IGDIWFEDGPYKSLNDAKLARSTIRACPKPPEPTSD"
     gene            complement(1289..1462)
                     /locus_tag="VGA15_25515"
     CDS             complement(1289..1462)
                     /locus_tag="VGA15_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591085.1"
                     /translation="MLSKSNEAAIDQIVASCNGDIRGALKALLLVNEHLEAELQQIYA
                     AVAHGVRGDSVLH"
     gene            1592..2374
                     /locus_tag="VGA15_25520"
     CDS             1592..2374
                     /locus_tag="VGA15_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEX9591086.1"
                     /translation="MNAADDGDKKAACSGKLRKSVIVFLVLLIGPIVVSAVKYLLGDR
                     SVDWQTADRSSAGLLPSPAGHPDALIRVYAARTVRWRGIFAVHSWIVIKELNAPRYTR
                     YDYTAWGAPIRINGFAADGRWFGGMPETIAAADGEAAERMIPKIRAAVEGYKLRSYGD
                     YRAWPGPNSNTFVTAVMAAVPELQTALPPTAIGKDFPYDGDVLGLTPSKTGVRITLGG
                     YLGLTIGWIEGIEINFLGAVFGIDLRRPAVKLPGLGRVGFGY"
     gene            2374..>2904
                     /locus_tag="VGA15_25525"
     CDS             2374..>2904
                     /locus_tag="VGA15_25525"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX9591087.1"
                     /translation="MRGLEHDSEKWVPVFPRDKREAFARRSCSNKKIERVDDSKKSHP
                     ALVGREHQRSCSCKLWSSVMENFTPLSAAIGGGLIGLSAVLLMLLTGRIAGISGIFGG
                     LLDFGNDDKGWRIAFIAGLILAPLMAGWIGHAMPTPKLPASWIVVIAAGLLVGFGTRL
                     GGGCTSGHGICGVARLS"
BASE COUNT          558 a          824 c          912 g          610 t
ORIGIN      
        1 caaacgataa gtgccgaaca cggcctttca aagcatcagg attgtcccat gaaggtccgt
       61 aactcgctga aatcgttgcg cggtcggcat cgcaacaacc gtctggtccg ccgcaagggc
      121 cgggtttacg tgatcaacaa ggtgcagcgc cgcttcaagg cacgccaagg ttgaatcaag
      181 cctgaattgg gccgttcggg tccattgcgc gaatctttct tcacgggtct ttgacgtctt
      241 gctgctttgc agcgcgtttt tctgcgtcta gactttggcc atgagattct cagctgcacg
      301 cgactgtctg gccgcgatcc tgttcaccgc gatgattgcg gcagcgccgg ctttggcgtt
      361 tgcgcaggat ccgggggcta cgccgccgaa accgcagaaa aaactgccgg aagcgccgag
      421 caagctgccg aaggtcggcg ccgatcgcac gcgcggactg gactttctgt ttggcgcact
      481 gaaagcggcg cccgacgagg ccagcgccaa gcatgtcgag gcgcggatct gggctttgtg
      541 gatgcagacc ccgagcgata cggctgcgct cttgatgatg cgcgccaggg ccgcgatgga
      601 cgcccagcag atggatgtcg cgttgaagct gctcgatgct gtcgtcaagc tgcgtcccga
      661 ttatgtcgag ggatggaacc ggcgcgcgac gctatattat ctgaagaacg actataccca
      721 ttcgctcgag gacatcgaac aggtgctggt ccgcgaaccg cgccactttg gtgcgctggc
      781 gggacttggc atgatcatgc aggatatcgg tgacgacaag cgcgcgctcg atgccttcag
      841 gaaggcattg gctgtcaacc cgcacctcga aaaagtgccg gagctggtca agaccctgag
      901 cgagaaggtc gagggccgcg atatctgatc ggcctgttga tggttagatc gtcaatgaat
      961 taaccacgat acccgacacc gcattgaggg ccggaacggt tttcgctatc gcggacttgc
     1021 ccgcttgacc gaagcgaggg agcgacagca tgaaatcatt gttgttggcc ggcgcgatga
     1081 tgtttgcgat cacgacggtt tgtttcgcgg aggagctcta ttgggtggtc ggcaaccgcg
     1141 cgaccaacag atgcgagatc gtcaccagca atccggttgt gatcggcgat atctggttcg
     1201 aagacggacc ctataaatcc ctcaacgatg ccaagctcgc gcgctccacc atccgcgcct
     1261 gcccgaagcc gcctgaaccg accagcgact agtgcaggac actgtcgccg cgcacgccgt
     1321 gagccaccgc cgcatagatc tgctgaagtt cggcctccag atgctcgttg accagcagca
     1381 gggctttcag cgcgccacgg atgtcgccgt tgcagcttgc cacgatctgg tcgatggcgg
     1441 cttcattcga tttggagagc atctcggatc cttcaacttt gccgtgagac aatctttcgc
     1501 gcctaaccat tgttcctgtg gatagtgtgg atagacgaca gcttcgatcg agcacgctcg
     1561 gctacatggc aaaactgctg atcacgcgta tatgaatgct gccgatgacg gcgataaaaa
     1621 ggcggcgtgt tcaggcaagc tgagaaaatc tgtgattgtc tttctggtgt tgctgatcgg
     1681 ccctatcgtt gtatcggctg tgaaatattt gctgggcgat cgctctgtcg attggcagac
     1741 ggcagatcgc tccagcgccg gactgctgcc gtctccggcg ggacatcccg atgccttgat
     1801 tcgggtctat gcggcgcgga cggttcgctg gcgcggaatt ttcgcggtgc atagctggat
     1861 cgtcatcaag gagttgaacg cgccgcgcta tacccgctac gattatacgg cttggggcgc
     1921 gccgatccgg atcaacgggt ttgccgccga tgggcgatgg ttcggcggca tgccggaaac
     1981 gatcgccgcc gccgacggcg aagctgccga gcgcatgatt cccaaaatcc gtgccgcggt
     2041 cgaaggctac aaattgcggt cgtacggtga ctatcgcgcc tggccgggac cgaattccaa
     2101 tacgtttgtc acagccgtaa tggcggcagt tccggaactg caaaccgcgc tgccgccgac
     2161 cgcgatcggc aaggactttc cttatgatgg cgacgtgctc ggactgacgc cttcgaaaac
     2221 gggtgtccgg atcaccctcg gcggttatct cggcctgacg atcggttgga tcgaaggcat
     2281 cgagatcaac tttctcggcg cggtgttcgg catcgatctg cggcggccgg ccgtcaaatt
     2341 gccggggctg ggacgcgtgg gctttggtta ttgatgcgtg ggctagagca tgattcggaa
     2401 aagtgggtac cggttttccc tcgcgacaaa cgcgaagcgt ttgcgcggag atcatgctca
     2461 aacaaaaaga tagagcgggt tgacgattcg aagaaaagtc atcccgctct agtcggccgg
     2521 gagcatcaga gatcgtgctc gtgcaagctg tggagtagcg tcatggagaa ttttaccccg
     2581 ctttcggcgg cgatcggtgg cggcctgatc ggcctgtccg ccgtcctact gatgctgctg
     2641 accggccgga tcgccggcat tagcggcata ttcggcggcc tcctcgattt cggcaacgac
     2701 gacaaaggct ggcgcatcgc ctttatcgca gggctcattc tggcgccgct gatggccggc
     2761 tggatcgggc atgcgatgcc aacaccgaaa ttgccggcaa gctggattgt cgttatcgcg
     2821 gccggcctgc tggttggttt cggcacgagg ctcggaggcg gctgcacctc cggccacggc
     2881 atctgcggcg tagcgcgcct gtcc
//
LOCUS       DASWGM010000946         4295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_957700,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000946 DASWGM010000000
VERSION     DASWGM010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957700"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGA15_25530"
     CDS             complement(<1..384)
                     /locus_tag="VGA15_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591088.1"
                     /translation="MCDYSLHAVATRPAQVGETLVSTTFRGTSTRGFASESAPAVAVC
                     MLPGTELAFAENVRYDNRWIWTKSVNFRVGKFGEIDPDVPHRHHDAIEFPDGSHVLVT
                     QLCEGQRVTVLQLPVVDRVTEHAQSA"
     gene            complement(740..1450)
                     /gene="lexA"
                     /locus_tag="VGA15_25535"
     CDS             complement(740..1450)
                     /gene="lexA"
                     /locus_tag="VGA15_25535"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX9591089.1"
                     /translation="MLTRKQYELLRFISERLKESGVPPSFDEMKDALDLRSKSGIHRL
                     ITALEERGFIRRLPNRARAIEVIKLPELSAGSGGNGRRGFTPSVIEGTLGRVRSSSSI
                     GSEDDGNRPVAVPVMGRIAAGTPIEALQTRSHTISVPPDMLGSGEHYALEVRGDSMVD
                     AGILDGDMALFQRNETAETGDIVVALIDEEEATLKRFRRRGASIALEPANTSYEVRIL
                     PPNRVKIQGKLIGLYRKY"
     gene            complement(1635..2846)
                     /gene="glp"
                     /locus_tag="VGA15_25540"
     CDS             complement(1635..2846)
                     /gene="glp"
                     /locus_tag="VGA15_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX9591090.1"
                     /translation="MALMPVTEALSAVLAGAEPLPEEMVALDAAHHRVLARDVAARRT
                     QPPQAMSAMDGYAVRAADASNVAARLKVIGEVAAGRPFDKTVGAGEAVRIFTGGVIPE
                     GADAVIIQEDTVAEGGGITITEAAVAGRHIRPAGVDFRQGDVLLARGIRLTDRDLSLA
                     AGMNYPELAVRRRPKVAILATGDELVMPGSTPGPGQIVYSNGYALRALARAEGAETID
                     LGIAADTVEATTQGIRRAGDSGADILITTGGASVGDHDLVKQSLEAEGVTMAFWRIAM
                     RPGKPMMHGRLGAMRVIGLPGNPVSSYVCGFLFLVPLIRKLSGRSVVHHTSETALLGR
                     SVAANDLREDYLRARLELRRDGVLVATPVDHQDSSLLGNLAAARALVIRAPFAAAAAA
                     GSACELLRLPD"
     gene            complement(2887..3516)
                     /locus_tag="VGA15_25545"
     CDS             complement(2887..3516)
                     /locus_tag="VGA15_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEX9591091.1"
                     /translation="MTRPLLSPGSADALLFDLGRVVLDIDFNKTLACWAGHAGCEPDD
                     IVARFVRDEAYKHHEIGKIGDDEYFASLRASLGINASNEQILEGWNAIFVGEMPGMSR
                     LLARASQRLPLYAFSNTNSAHVEHFSQAYADVLSHFREMFLSSTIGLRKPDAAAYDHV
                     VKAIGVPASRIVFFDDLAENIEGARARGLTAVHVTSPDDVAHALAALGI"
     gene            complement(3939..4292)
                     /locus_tag="VGA15_25550"
     CDS             complement(3939..4292)
                     /locus_tag="VGA15_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591092.1"
                     /translation="MTLFPNLLQPDVALKTYAPLSIKFWENQGSALEGMKEFADGWFA
                     RRHKTTQAGLEAAKRMGDASTHFDIMREYQTWLTGAMEAFAEDGKAYQQQLLRTGFQL
                     NPKPEARQADERRAG"
BASE COUNT          809 a         1338 c         1376 g          772 t
ORIGIN      
        1 agcggactgc gcatgctccg taacccgatc aaccaccggc aattgcaaga ccgtcacgcg
       61 ctgcccttcg cagagctgcg tcaccagcac atggcttcca tctggaaatt cgatcgcatc
      121 atggtgacga tgcggaacat cggggtcgat ctcgccgaat ttcccaaccc ggaaattgac
      181 tgatttcgtc cagatccatc tgttgtcgta gcggacgttt tcggcgaacg ccagttcggt
      241 cccgggaagc atgcagaccg ccaccgcggg cgcactttcc gaggcgaaac cacgagtcga
      301 ggttccgcgg aatgtcgtgg aaacgagcgt ctcgccaacc tgggcggggc gcgttgctac
      361 agcgtgcaga ctatagtcac acatcggttt gctcctcttt cgagatagcc gaccggcgcc
      421 gcctagaggc gcaggcctct atcagagtgg acagtagcac gtgagccttt gcccggttgt
      481 gggcaaatgt gcggctccgg ccgacgagac tgatcacaaa tatgttttaa gaggctggac
      541 cgacgcgggc aggtgatgga tgccaagctg gagcctgtca tcgggctcgc cgaaggcgga
      601 ggcccggtgg cggggcgcga cggcagaggt gagtggcgcg acgatcagcg agacgtcaat
      661 ccccgacccg caatcggcct gtcccaggtc ttttcgggat gggtcttttc gggatgggtc
      721 ttttcgggat catgcccgct caatacttcc ggtaaagccc gatcagcttg ccctgaatct
      781 tgacccggtt cggcggcagg atgcggactt cataggaggt gttggcgggc tccagcgcga
      841 tcgaggcgcc gcggcggcgg aagcgcttga gcgtggcttc ctcttcatcg atcagcgcca
      901 ccacgatgtc gccggtctcg gcggtctcgt tgcgctggaa cagcgccatg tcgccatcga
      961 gaatccctgc atccaccatg gaatcgccgc gcacctccag ggcgtagtgc tctccggaac
     1021 cgagcatgtc cggtggcacg ctgatggtgt gactgcgggt ctgcaacgcc tcaatcggcg
     1081 tgccggcggc gatgcggccc atgaccggca cggcaaccgg acgattgcca tcgtcctcgg
     1141 aaccgatgct ggagctgctg cggaccctgc cgagcgtgcc ctcgatgaca ctgggcgtaa
     1201 aaccgcggcg gccgttgccg ccactgccgg ccgaaagctc gggcagcttg atgacctcga
     1261 tggcgcgggc gcgattggga aggcggcgaa tgaagccgcg ctcttcaagg gctgtgatga
     1321 ggcgatggat cccggacttc gaacgcaaat ccagcgcatc cttcatctcg tcgaaggagg
     1381 gcggaacgcc agattccttc aagcgttcgc tgatgaatcg caggagttcg tattgtttgc
     1441 gcgtgagcat ctcgaccaat ccccaggttt gatgtcgttc gattccaaga cgcgggagtt
     1501 agctattccc aattccaagc gtctgatact cgaaacaaat catgaacaga ccctatatgt
     1561 tccatatgtg ttccgcaacc acttaatttt gcgtcaacga accagttttg cgtgaatgcg
     1621 ccgcctaaag ggaatcagtc cggaagccga agcagttcgc aggccgatcc ggcggcggcg
     1681 gcggcggcga acggcgcacg gatcacaagc gcccgtgccg cagcgagatt tcccaacaac
     1741 gaagagtcct gatggtcgac cggcgtggcg accaggacgc cgtcgcggcg cagctcgagg
     1801 cgggcgcgca gatagtcttc gcgcaaatcg ttggcggcga cgctgcgtcc gagcagcgcc
     1861 gtctcgcttg tgtggtggac gacgctgcgg cccgaaagct tacgaatcag cggcaccagg
     1921 aacaggaatc cgcagacgta ggacgatacg ggattgccgg gcaggccgat cacccgcatt
     1981 gcgcccagcc gtccatgcat catcggcttg cccgggcgca tcgcgatccg ccagaacgcc
     2041 atggtgacgc cctcggcttc cagcgattgc ttgacgaggt cgtgatcgcc gaccgaggcg
     2101 ccgccagtcg tgatgaggat gtcggcgccg ctgtcgccgg cgcggcggat gccttgcgtg
     2161 gtggcctcga cggtgtcggc ggcaatgccg agatcgatgg tctcggcacc ttcggcacgc
     2221 gccagcgccc gcagcgcata gccgttggaa tagacgatct gcccggggcc gggggtcgag
     2281 cccggcatca ccagttcgtc gccggtggcg aggatcgcga ccttgggtcg gcggcgcacc
     2341 gcgagttcgg gatagttcat gccggcggcc agcgacagat cgcggtcggt gaggcgtatt
     2401 ccgcgtgcca gcaggacatc gccttggcga aagtcgacgc cggccgggcg gatatgccgt
     2461 ccggcgaccg cggcttcggt gatggtgatg cctcccccct cggccacggt gtcttcctga
     2521 atgatgacgg catcggcacc ttcggggatc acgccgccgg taaaaatccg caccgcctcg
     2581 ccggcgccaa cggtcttgtc gaacggacgg cccgccgcga cctcgccgat caccttgagc
     2641 cgcgccgcaa cgttcgacgc gtcggccgcg cgcaccgcat agccgtccat cgccgacatc
     2701 gcctgcggcg gctgggtgcg ccgtgccgcg acgtcccgtg ccagcacgcg gtgatgcgcc
     2761 gcatcgagcg cgaccatctc ctcgggcagc ggctcggcgc ccgcgagaac cgccgacagc
     2821 gcctcggtaa ccggcatcag tgccaagata aaactcctaa agcgaaaact cctaaagcca
     2881 aaccttctag ataccgagcg cggccagcgc gtgcgctaca tcgtcaggtg atgtcacgtg
     2941 aacggcggtc aagccccgcg cccgcgcgcc ttcgatattc tcggcgagat cgtcaaagaa
     3001 cacgatgcgg gaggccggta cgccgatggc tttcaccaca tgatcatagg ccgccgcatc
     3061 cggcttccga agcccgatcg tggacgacag gaacatttcg cggaaatggc tgagcacatc
     3121 ggcataggct tgcgagaaat gttcgacatg agcgctgttg gtattggaga aggcgtagag
     3181 cggcaggcgc tgcgatgccc gcgccagcag ccgcgacatg ccgggcatct cgccgacaaa
     3241 gatggcattc cagccttcga gaatttgttc gttcgaggcg ttgattccca gcgacgcccg
     3301 caggcttgca aaatactcat catcgccgat cttgccgatc tcatgatgct tgtaggcctc
     3361 gtcgcggaca aagcgcgcaa cgatgtcgtc gggctcacag ccggcatgac cggcccagca
     3421 ggccagcgtc ttgttgaaat cgatatcgag cacgacgcgg ccgagatcga acaatagcgc
     3481 gtcggccgag ccgggggaga gcagggggcg ggtcatgggg gttgtgtacg aaaatcaggg
     3541 gagaggagca acggtgtggc gatgtgcgct gatccaattt gcggattcaa gtcagacgtc
     3601 tcgccagatc cgaaaagcaa agatatggac cggccggtcg cgccggcgca tcggcctcgt
     3661 cgaatctgat acggcgcaga gagcgctggc tcgcgcgtcg gcgcgatagg caaacgatgc
     3721 ccgtgcgggc ggttcccgct ctgctggcga tggagatggc ccgctttccg acgcccagtc
     3781 atcggcgcaa acgcaccctg ccggcatcca acagcccgca aggctggata ccgttaccag
     3841 aacgcgcgcg tcgtcgtcca cacgacaggg ctgtcaacgc ggtgccgcgc taaccagggg
     3901 gtgtggggga cgtcgactgt cgctttatga cagagccgtt agccggcccg acgctcgtcg
     3961 gcctggcgcg cttccggctt tggattcaac tgaaacccgg tcctcagcag ttgttgctga
     4021 taggccttgc catcctcggc aaacgcttcc atcgctccgg tgagccaggt ctgatattcc
     4081 cgcatgatat cgaagtgcgt cgaggcatcg cccatacgct tggcggcttc aagccccgcc
     4141 tgcgtggtct tgtgacgccg ggcgaaccag ccatcggcaa attctttcat gccttcgaga
     4201 gcgcttccct ggttttccca gaacttgatg ctcagaggcg cataggtctt caaagccaca
     4261 tccggctgca agagattcgg aaacagtgtc acgtt
//
LOCUS       DASWGM010000947         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1917098,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000947 DASWGM010000000
VERSION     DASWGM010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1917098"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGA15_25555"
     CDS             <1..289
                     /locus_tag="VGA15_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEX9591093.1"
                     /translation="LPPLGIFMSEFLVVSSTFARQPLLAIVLVLGLLLAFGALMLRLT
                     SVAFGNPRGSTASAEASYLPMFAHLSLVLVAGIYLPAPLVVWFQHVARLLG"
     gene            309..1820
                     /locus_tag="VGA15_25560"
     CDS             309..1820
                     /locus_tag="VGA15_25560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX9591094.1"
                     /translation="MPSLIDLMLEGRAVQHHGRWPRATVDADLWKFAAGEVARRRWSL
                     LDLWGEPATVHMAIMDEATGEIATVSLDCPDLTFPSVGLHHPPALRLERAINDLFGLS
                     AEGLPDARPWLDHDCWGVGFPLGNRVYGLSKTSPYRFLPAEGDGLHQIAVGPVHAGII
                     EPGHFRFTASGETVVRLEQRLGYTHKGIEGLMIGADIERAVELAGRVSGDSTVAYAYA
                     FSRAAEATSQLVVPDRAVWLRALLAELERLANHLGDIGAICNDASFTLMHAHCSVLRE
                     SVLRASNGAFGHRLLRDVIVPGGVTHDLSEEATKLIQAMLGNIRLRFPALVELYDNTA
                     SLQDRTVDTGVLKPALAQQYAAGGYIGRASGRSFDARRTLAYPPYDSLRFDVPVLNEG
                     DVNARVWIRVREVEQSLSLIDQILDRLPQGPLRVQADQLREPGEGMAIVEGFRGDVLA
                     WLRVRDGRVERCRLRDPSWFQWPLLEACIENNIVADFPLCNKSFNCSYSGQDL"
     gene            1870..2418
                     /locus_tag="VGA15_25565"
     CDS             1870..2418
                     /locus_tag="VGA15_25565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX9591095.1"
                     /translation="MRKLLFQSLFRRPFTEQAPRLDDVALSELATAVGNAARRRLGRS
                     LSIREVDAGSCNGCELEIHALNNAYYDVERFGLRFVASPRHADVLMVTGPVTKNMREA
                     LERTYHAVPNPKWVVAIGDCGRDGGCFAGSYAVVGGVSEVIPVDLHIPGCPPTPTAML
                     QGLLALLERADGRAFSGQVESG"
     gene            complement(2469..2789)
                     /locus_tag="VGA15_25570"
     CDS             complement(2469..2789)
                     /locus_tag="VGA15_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9591096.1"
                     /translation="MITANQLRAARALLNIDQRQTADLADLSVPTIQRMEASDGVVRG
                     NVDSLMKLVSALENAGIELINPGVASVAGGRGVRLREHVPKPKLKTVRPPKPSSSRTM
                     ERAR"
     gene            3010..>3523
                     /locus_tag="VGA15_25575"
     CDS             3010..>3523
                     /locus_tag="VGA15_25575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX9591097.1"
                     /translation="MRLLGRYQGRNGAIEIVECLGDGTRIYFEEGVRQSQATPDGESV
                     FTYVKLMDELLYRAKNILVLGCGGGNLPTRLARLGKRLTIVDNNPISFVIAHKFFGLP
                     DDLPCVVSDFRRFVYQDDTLYDGIAIDVGGPGFEFNDEFDLDTCDAIRARLVPGGRIV
                     MNVTVSNDIDP"
BASE COUNT          642 a         1062 c         1094 g          725 t
ORIGIN      
        1 actgccgccg ctcggcattt tcatgagcga atttctcgtc gtcagctcga cctttgcgcg
       61 gcagccgctg ctcgcgatcg tgctggtgct cgggctgttg ctggcgttcg gcgcattgat
      121 gctgcgcctg accagcgtcg cattcggaaa cccgcgcggc agcaccgcat cggccgaggc
      181 ttcgtatctc ccgatgttcg cgcatctctc actggttctg gtcgcgggaa tctatcttcc
      241 cgcgccgctc gtcgtctggt tccagcatgt ggctcgcctt cttggatagt tttttaggat
      301 taaccgatat gccatcgctg atcgatctca tgctggaggg ccgcgcggtc cagcaccacg
      361 gccgatggcc gcgggccacg gtcgatgcgg acctctggaa atttgccgcc ggcgaagtgg
      421 cacgccggcg ttggagcctg ctcgacctct ggggagaacc cgccacggtg cacatggcga
      481 tcatggatga agcaacgggc gaaatcgcca ccgtcagcct ggattgtccc gatctcactt
      541 ttccctcggt cggcttgcac cacccgccgg cgcttcgact ggagcgcgcc atcaacgatc
      601 tgttcgggtt atcggcggaa ggcttgcccg atgcccgccc ctggctggat cacgattgtt
      661 ggggcgtggg cttcccgttg ggaaatcgcg tctacgggtt gtcgaagaca tcgccctatc
      721 gcttcctgcc ggcggaaggc gacggcctgc atcaaatagc ggtcggtccg gtgcacgcgg
      781 ggatcatcga gcccggacat ttccgcttta ccgccagcgg cgagaccgtg gtccggctgg
      841 agcagcggct cggatacacc cacaagggca tcgaggggct gatgatcgga gccgatatcg
      901 agcgggccgt cgaactcgcc gggcgcgtat caggcgacag cacggtcgcc tatgcctatg
      961 cgttttcgcg ggccgctgag gccacctcgc agctcgtcgt gcccgatcgc gcggtttggc
     1021 tgcgggcgct gctcgccgaa ctcgagcggc ttgccaacca tctcggcgac atcggcgcga
     1081 tctgcaacga tgcctccttc accctgatgc acgcgcattg cagcgtgttg agggagagcg
     1141 tactgcgcgc atccaacggc gccttcggtc accggttgtt gcgggatgtc atcgtgcccg
     1201 gcggcgtgac ccacgacctc agcgaagaag cgacaaaact gatccaggcg atgttgggca
     1261 atatccgcct gcgctttccg gccctggtcg aactctacga caacaccgcg tcgctgcagg
     1321 atcgcaccgt cgataccggc gtgctcaaac ccgcattggc ccaacaatat gccgcggggg
     1381 gctatatcgg ccgcgcctcc ggccggtcct tcgatgcgcg ccggaccctg gcctatccac
     1441 cctatgacag cctgcgtttt gacgtaccgg tgctgaacga gggtgatgtc aatgcccggg
     1501 tctggattcg cgtccgcgag gtcgagcaga gtctttcgct gatcgaccag atattggacc
     1561 gactgccgca aggccccctg cgggtgcaag cagaccaact gcgagaaccc ggggaaggca
     1621 tggccatcgt cgagggattt cgcggcgatg ttctggcctg gctgcgcgtg cgcgatggcc
     1681 gggtcgagcg atgccgcctg cgcgacccca gttggtttca atggccgctg cttgaggctt
     1741 gcatcgaaaa caacatcgtt gcggattttc cgctctgcaa caagtcgttc aactgttcct
     1801 actccggcca ggatctctga gaacagcttc gctgaaaact taaggacagc tcttcaagga
     1861 cagcacgaaa tgcgaaaatt gctttttcaa agcctgtttc gccggccatt caccgaacag
     1921 gcgccgcgat tggacgatgt cgcgttgagc gaactcgcaa ccgccgtcgg caacgcggcg
     1981 cggcggcggc ttggccgcag cctttccatc cgcgaggtcg atgccggatc ctgcaacgga
     2041 tgcgaactcg agattcacgc gctcaacaac gcctactacg atgtcgaacg cttcggcctg
     2101 cggttcgtcg cctcgccgcg ccatgccgat gtgttgatgg tgaccggtcc cgtgaccaag
     2161 aacatgcgcg aagcgctgga gcgcacctat catgcggtgc ccaatcccaa atgggtggtc
     2221 gcgatcgggg attgcggccg tgacggcggg tgttttgccg gcagctacgc ggttgtcggc
     2281 ggcgtttccg aggtgatccc ggtcgatctg cacatacccg gctgcccccc taccccgacg
     2341 gcgatgctgc aaggtctgct ggcgctgctc gaacgggcgg acggcagagc attttccggc
     2401 caagtggaat ccggttagcg ctaagaaaat gcgtttaact gggaacttgc gcggttacat
     2461 cctgatcttt atcgcgcccg ttccatggtc cgcgaggaag acggctttgg cggccggacc
     2521 gtcttcagct ttggttttgg aacatgctcg cggagtcgca cgccgcggcc tccagccaca
     2581 ctcgccacgc ccggattaat caactcgatg cccgcgttct caagcgcgga aaccagtttc
     2641 atgagagaat cgacgttgcc gcggacgacg ccgtcactgg cctccatgcg ctggatcgtt
     2701 ggaaccgaaa ggtctgccag atcggccgtc tgccgctgat cgatgttcag gagcgcgcgg
     2761 gcggccctga gttgattggc agtaatcatg aacctggttt ctcccaatct gaagcaagca
     2821 tgattacacg gtacgatacg ggcctcaagc atcaatttac aagcataaga tatcaaggtg
     2881 ccctggcgca atcgttgcaa attcgcgggg atcggcggca ggcgcgattg gcatagggca
     2941 cattacatga taaaagatgt gtaatgcatg ctatttaatg cataacatca aacagatggg
     3001 ttgtcggctg tgcggttgtt ggggcgctac cagggcagga acggcgcgat cgagatcgtc
     3061 gaatgtttag gggatggaac ccggatttat ttcgaagagg gcgtgcggca gagccaggcg
     3121 acgcccgacg gtgagagcgt attcacctac gtcaagctga tggacgagct gttgtaccgc
     3181 gcgaaaaaca tcctggtgct gggatgcggc ggcggaaacc tgcccaccag gctggcccgt
     3241 cttggaaaac gactgacgat cgtcgacaac aatccgatca gcttcgtgat cgcgcacaag
     3301 ttttttgggc tgccggatga tctgccgtgc gtggtctccg atttccgcag gttcgtctat
     3361 caagacgata cgctttacga cggcatcgcg atcgatgtcg gcggtccggg ttttgagttc
     3421 aacgatgaat tcgatctcga tacctgcgat gcgatccgcg cacggctggt gccgggcggc
     3481 cgcatcgtga tgaacgtgac ggtatcgaac gatatcgatc cca
//
LOCUS       DASWGM010000948         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_796780,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000948 DASWGM010000000
VERSION     DASWGM010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796780"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VGA15_25580"
     CDS             <1..1033
                     /locus_tag="VGA15_25580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591098.1"
                     /translation="SIWPLSLMRNAVDDLEAARLEKYDADLARVPANAPLWDWTPFLD
                     TQDSTKRAKVLDSIRSIDQRQAQAETMLDRGDFPIGYLGSFDLDPTPALCEKARSQLR
                     RRVEPLVLKTADPKPYSDIALPLADAISGMNWLVGYGCACDAEAAAWEAMAKGYSNTS
                     LDVYHLAELRDPRELGRTLRERPERFSMLNAKSHLKGWLRFADDESLHDQALTGARVL
                     DHRTTDAVEILIDKYDEESRWKLLRYLTALDLDATMPLCANLLSELHGQFTKVYRPGA
                     NDEPRPYSELLERLGNGEQLPDLIWLAGHGCAAEPELSEAVALVNAYRDSLGRAEMLA
                     TLTRLRGRR"
     gene            complement(1041..1502)
                     /locus_tag="VGA15_25585"
     CDS             complement(1041..1502)
                     /locus_tag="VGA15_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9591099.1"
                     /translation="MHTLDAIDRKILSQLQSDSRTTMQELADKVGLSVSPCHRRVKLL
                     EERGVITRYIATVDQKSLGLHVSVFISIKLARQKEEDLNRFAKAISKWDEVLECYLMT
                     GNRDYLLRVVAADLSSYEAFLKNKLTRLDGIASIESSFALSQVKYSIALPV"
     gene            1641..>2876
                     /locus_tag="VGA15_25590"
     CDS             1641..>2876
                     /locus_tag="VGA15_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX9591100.1"
                     /translation="MPVEPARLEMLSALARKVLWLSSWTIHHANHVRPNVDGLKVGGH
                     QASSASLATIMSALYFSVLRPEDRVAVKPHASPVFHAIQYLFGRQTRDKLENFRGFKG
                     AQSYPSRTKDTDDVDFSTGSVGLGVAQTLFSSLVQDYVKAHGWMKERPEGRMIALVGD
                     AEMDEGNIFEALLEGWKHGLRNTWWVVDYNRQSLDAVVREGLWEKFESMFRNFGWDVV
                     IVKYGKLMQAAFAEAGGEALKRWIDDCPNAMYAALCFQGGAAFRKHLTDEIGDQGAVS
                     QLIDRRSDDELLALMSNLGGHDMASMVEAFEAIDHDRPVCFIAYTIKGVGLPFQGHKD
                     NHAGLMTVAQMEKWRSAQNIRPGHEWEKFEGLSQDPAALEAFLGQAPFNRDGRRRLTA
                     PVIEVPEQLSFKPSRQMSTQ"
BASE COUNT          562 a          842 c          930 g          542 t
ORIGIN      
        1 ttcgatctgg ccgctgtcgc tgatgcgcaa cgcggtggac gatctggagg ccgcacggct
       61 ggaaaaatac gacgcggatc tcgcccgtgt gccggcgaat gctcccctgt gggattggac
      121 gccattcctc gatacccaag atagtaccaa gcgggcgaag gtgctcgaca gcatacgcag
      181 catcgaccag cgccaggctc aagccgaaac catgctcgat cgcggcgact ttcccattgg
      241 ctatctcggg tccttcgatc tcgatccaac gcctgcgctt tgcgagaagg cgcgcagcca
      301 gcttcgcagg cgcgtcgaac cgcttgtgct caaaaccgcc gatcccaaac cgtattccga
      361 tatcgcgctg ccgttggcag acgccatctc aggcatgaac tggctggtcg gctatggttg
      421 cgcgtgcgac gccgaagcgg cggcctggga agccatggcc aaaggataca gcaacaccag
      481 tttggacgtc tatcatctgg ccgagcttcg cgatcccagg gaactggggc gaacgctgcg
      541 cgagcgcccg gagcgctttt caatgttgaa cgcgaagtcg cacctgaagg ggtggctgag
      601 atttgccgac gacgagagct tgcatgatca ggccctgacc ggcgctcgcg tgctcgacca
      661 ccgcaccacc gacgcggtcg agatattgat cgataaatat gacgaggaaa gccgatggaa
      721 gctgttgcga tatcttaccg cgctcgacct cgatgcgacg atgcccctgt gcgcgaacct
      781 gctgagcgag ttgcacggtc agttcacgaa ggtctaccgg ccaggcgcga atgatgagcc
      841 acggccttat tccgaattgc tcgagcggct gggaaacggc gagcaactgc cagacctgat
      901 atggctggcc ggacacggtt gcgcggctga gccggaattg agcgaggcgg tggcgctggt
      961 gaatgcctat cgggattcgc ttggcagagc agagatgctg gcgacgctga cgcggcttcg
     1021 cgggcggcgg tgacgaaaga tcacaccggc agcgcgatcg aatacttcac ctggctcaac
     1081 gcaaaactcg actcgatcga ggcgatgccg tcgagccggg tcagcttgtt tttcaagaac
     1141 gcttcgtacg acgacaggtc ggcggcgacg acgcgcaaca ggtagtcgcg attgccggtc
     1201 atcaggtagc actccaaaac ctcgtcccat ttcgagatcg ctttcgcaaa ccggttgagg
     1261 tcctcttcct tttgccgcgc cagcttgatc gagatgaaca cgctgacatg caggccgagc
     1321 gatttctggt cgacggtcgc gatatagcgg gtgatcacgc cgcgctcttc cagaagtttc
     1381 acccggcgat ggcagggcga gaccgacagg ccgaccttgt cggcgagctc ctgcatggtg
     1441 gtgcggctgt cggattgcag ctggctgagg attttgcggt cgatggcgtc aagcgtatgc
     1501 attaggataa actctggcta gcgggctccg aagtggggtt ttatcccaat ttttggcggt
     1561 atgtcgcgag aatttgagat ttttggcgaa agccgaagcg ctaccattgg cggataatca
     1621 ctgtgccgga gaataccgcc atgcccgttg agcccgcacg tcttgaaatg ctgtccgcgc
     1681 tggcccgcaa agtcttgtgg ctgtcgtcgt ggaccatcca tcacgccaat cacgtcaggc
     1741 ccaacgtcga cggcctgaaa gtcggcggcc atcaggcctc gtcggcctcg ctcgccacca
     1801 tcatgtcggc gctatatttt tcagtgctgc ggccggaaga ccgcgtcgcg gtgaagccgc
     1861 atgcgagccc ggtgtttcac gccatccagt acctgttcgg gcgccagacc cgcgacaagc
     1921 tggaaaactt tcgcggcttc aagggcgcgc agtcctatcc gtcgcgcacc aaggacaccg
     1981 acgacgtcga tttctccacg ggatcggtgg gcctcggcgt cgcgcagacg ctgttctcct
     2041 cgctggtgca ggattacgtc aaggcgcatg gctggatgaa ggagcggccc gaggggcgga
     2101 tgatcgcgct ggtcggcgac gccgagatgg acgagggcaa catcttcgag gcgctgctgg
     2161 agggctggaa gcacgggttg cgcaacacct ggtgggtggt cgactacaac cgccagagtc
     2221 tcgatgccgt ggtgcgcgag ggactgtggg aaaaattcga atccatgttc cgcaactttg
     2281 gttgggacgt ggtcatcgtg aagtacggca agctgatgca ggcggctttc gcggaagcag
     2341 gcggtgaggc cctaaaacgc tggatcgacg attgcccgaa cgcgatgtat gccgcgctgt
     2401 gctttcaggg cggggccgcg ttccgcaagc atctcacgga cgagatcggc gatcagggcg
     2461 cggtgtcgca gctgatcgac aggcgcagcg acgatgaatt gctggcgctg atgtccaatc
     2521 tcggcggaca cgacatggcc agcatggtcg aggccttcga ggccatcgat cacgaccggc
     2581 cggtctgctt tatcgcctac accatcaagg gcgtcggcct gccgttccag ggccacaagg
     2641 acaaccatgc cggcttgatg acggtcgccc agatggaaaa atggcgcagc gcgcagaaca
     2701 tccggccggg tcacgaatgg gaaaaattcg aggggctgtc tcaggatccg gcggcgctgg
     2761 aggcgtttct tggccaggcg ccgttcaatc gcgacggccg gcgccgcctg accgcgcccg
     2821 tgatcgaggt gcccgagcag ctttcgttca agccgtcgcg gcaaatgtcg acccag
//
LOCUS       DASWGM010000949         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1214214,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000949 DASWGM010000000
VERSION     DASWGM010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214214"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VGA15_25595"
     CDS             complement(<1..174)
                     /locus_tag="VGA15_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX9591101.1"
                     /translation="MDFNMSDRQREWLNRVESFMHKHVRPAVPIYKQQDEAGERWKVI
                     PILEDLKKKARAEG"
     gene            410..1078
                     /locus_tag="VGA15_25600"
     CDS             410..1078
                     /locus_tag="VGA15_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9591102.1"
                     /translation="MPSDHTRAAILNSAERLYADRGFADVTLRDIVAVADVNLAAVNY
                     HFGSKDELIAELFVTRSLATNRERLNELKAAEEKGGGRASIDAILRALVGPTLRGCLG
                     PERERSTAARFMIRASIESVPPIRRIKNREIDHLRKFAAAMRRALPDRDEVDLYWGLH
                     FALAMSHQTIRDSERLIRLSEGKCDLNDVAGIIERIVAVSVMALTGGESKIADKLSVQ
                     RARQ"
     gene            complement(1095..1487)
                     /locus_tag="VGA15_25605"
     CDS             complement(1095..1487)
                     /locus_tag="VGA15_25605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9591103.1"
                     /translation="MKREAIRVEPISSYLAKWKAPISPVTRGGGMVFVSGLPPFDPET
                     GEILPVPIERQTELILEQMKLCLETAGTSLQNVMKCNIYCTSAKHFATVNAIYARYFP
                     LDPPARIFVCVPEWTGPFDIEIDCVAVV"
     gene            complement(1616..>2731)
                     /locus_tag="VGA15_25610"
     CDS             complement(1616..>2731)
                     /locus_tag="VGA15_25610"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HEX9591104.1"
                     /translation="FAILLQTASASEAVATAHRVTIALNGKYELDQYGEANVGVSIGI
                     ACAPAHANEPEAPLSHADKALYAAKSAGLGIPRLYDDHLKSETKTASSASPPGKPRRD
                     SSGLQALREAANLANDLRVAMHAGHLHLHYQPIYDTCTRRLIACEALLRWNDPIRGNV
                     SPLEFIPIAEDSGLIGPLTEWVLLHACEEAAAWPDAVQVSINLSPLNLSQPSLVTTVT
                     RVLGDTGLAPQRLILELTEGLLLEKSNTVRDALLGLKDLGVELWLDDFGAGHANLGYL
                     QRLSCNVVKIDRGFLDQHDKRRELLGGMISLAQACGLRVLAEGVETIEHDALLKELGC
                     DLLQGYLLARPMSAESLRALISASDFSVSESPSLALT"
BASE COUNT          551 a          820 c          830 g          530 t
ORIGIN      
        1 gccttcggcg cgcgccttct tcttcagatc ttccaggatc gggatcacct tccagcgctc
       61 gcccgcttcg tcctgctgct tgtagatcgg caccgcgggc cggacatgct tgtgcatgaa
      121 cgattccaca cggttgagcc attcgcgctg acggtcagac atgttgaagt ccattgggcg
      181 ttcctcacat ttttgggcga aaaattgttg gcggcactgt ctccccgcag cctctgcacc
      241 gcaagactat tttgcagcgc tttgtcagca cggcgatggc ggccggcgtg gttttggctg
      301 gcctggcgat gcgtcatgcg cattgacatt ccagccttca aaccatagtt tcatacaagt
      361 gtttgaaatg caaccgccgc atttcacgct gatcgcacag ggccatgaca tgccgtcgga
      421 tcacaccaga gccgcgatcc ttaactccgc cgaacggctt tacgcggacc gcggtttcgc
      481 ggacgtcacg ctgcgcgaca tcgtcgccgt cgccgacgtc aatctggcgg cagtgaacta
      541 tcatttcggc tccaaggacg agttgatcgc cgaactgttc gtcacccgca gcctcgccac
      601 caaccgcgaa cggctcaacg aattgaaagc ggcggaagaa aaaggcggcg gccgcgccag
      661 catcgacgcg atcttgcgcg cgctggtcgg accgaccttg cgcggctgtc tcggccccga
      721 gcgcgagcgc tcgaccgcgg cgcgcttcat gatccgcgcc tcgatcgaat cggtgccgcc
      781 gatccgccgc atcaagaatc gcgagatcga tcacttgcgg aaattcgccg cggcgatgcg
      841 gcgcgcactg cccgaccgcg acgaggtcga tctctattgg ggcctgcatt tcgcgctcgc
      901 catgtcgcac cagaccatcc gcgacagcga gcgattgata agactgtcgg aaggaaagtg
      961 cgatctcaac gacgtcgccg gcatcatcga gcgcatcgtt gcggtgtcgg tgatggcgct
     1021 gaccggtggc gagagcaaga tcgccgataa gctcagcgtg cagcgcgcgc gccaataatg
     1081 gctggcgtgg aaagtcagac cacggcgacg cagtcgattt cgatgtcgaa cggcccggtc
     1141 cattccggca cgcagacgaa gatgcgcgcg ggcggatcta acgggaagta acgcgcgtag
     1201 atcgcgttca cggtcgcaaa gtgttttgcg gatgtgcagt agatgttgca cttcatcacg
     1261 ttctgcaaac ttgttcccgc cgtctccagg cacagtttca tctgctcgag gatcagttcg
     1321 gtctggcgct cgatcggcac cggcaaaatc tcgccggttt ccggatcgaa cggcggcagt
     1381 cccgaaacga agaccatgcc gccgccacgc gtgaccggcg agatcggcgc cttccacttc
     1441 gccagatagg aggaaatcgg ttcgacgcgg atggcttctc gtttcatggc aacctcggct
     1501 gggatcgatg atgatcgcaa actagcacgt gtcgcgacca acatgcttcg agctcgctgg
     1561 aagtgtgcta tggagcggag gcgttgacgc gaggccccac gatctacccg gcgatctagg
     1621 tgagtgccag gctgggactt tcgctgacgc tgaaatccga ggccgaaatc agcgcacgca
     1681 aactttcggc ggacatcgga cgggccaaaa gatacccctg cagcagatca cagccgagct
     1741 ctttgagaag cgcgtcgtgt tcaatggtct cgacaccttc ggccagaaca cgaaggccgc
     1801 acgcttgcgc cagtgaaatc atgccaccga gaagctcgcg acgcttgtcg tgctgatcga
     1861 ggaagccccg gtcgattttc acgacgttgc acgacagtcg ctgcaggtaa ccgagatttg
     1921 catggccggc gccaaaatca tcgagccaca gctcgacacc aagatccttc aggccgagaa
     1981 gcgcgtcccg aacggtgttc gacttctcga gcagcaaacc ttccgtcaac tcgagaatga
     2041 gccgctgcgg cgcaagaccg gtatcgccga gaacgcgggt aaccgtcgtt acaagggaag
     2101 gctggctcag attaagcggt gaaagattga tgctgacctg tacggcatcg ggccaggcgg
     2161 cggcttcttc gcaggcgtga agcaaaaccc actccgtcaa cggaccgata agcccgctgt
     2221 cttcggctat cggaataaac tcaagcggcg agacatttcc cctgatcggg tcgttccagc
     2281 gcagcagggc ctcgcaggcg atcaggcgcc tcgtgcacgt atcgtagatt ggctggtagt
     2341 gaaggtggag atgaccggca tgcatggcga cccgcaggtc gttggcgaga tttgcagcct
     2401 cccgaagcgc ttgcaggccg gacgaatccc ggcggggttt tcccggtggc gaggcagacg
     2461 acgcggtctt ggtctcactc ttcagatgat cgtcataaag acgaggaatt ccaagcccgg
     2521 cgctctttgc agcatagagc gctttgtcgg cgtgcgataa cggtgcctcg ggttcgttgg
     2581 catgggccgg cgcgcaggca atgccaatgc tgacaccaac gtttgcttcg ccgtattggt
     2641 cgagttcata tttgccgttc agcgcaatgg tcacgcggtg cgccgtggcg acagcttccg
     2701 aggcggaagc ggtttgcaac agaatagcga a
//
LOCUS       DASWGM010000950         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1078641,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000950 DASWGM010000000
VERSION     DASWGM010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078641"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VGA15_25615"
     CDS             complement(<1..290)
                     /locus_tag="VGA15_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX9591105.1"
                     /translation="MNGKQSQYSIGLDKTPANFVPLTPLSFLARTAAIYPSHLSAVYE
                     GRAFTWSQTYSRCRRFASFLASRNIADGDTVAAMLPNIPAMNEVHFAVPMAGA"
     gene            complement(315..>1715)
                     /locus_tag="VGA15_25620"
     CDS             complement(315..>1715)
                     /locus_tag="VGA15_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX9591106.1"
                     /translation="MALFAFLRLARAGDIRTVLGAVLSVALLIAVFWLAQPWLRPLGN
                     INLIIFFVGVAGFCVFAGVPIAFGFGLAIYGYLALTTHTPLIVLVGRMDEGMSHLILL
                     SVPLFVFLGLLIEMTGMARAMVAFLASLLGHVRGGLHYVLVGAMYLVSGISGSKAADM
                     AAVAPVLFPEMKARGAKPGDLVALLAATGAQTETIPPSLVLITIGSVTGVSIAALFTG
                     GLLPGVVLAITLSALVWWRYRGEDLSHVKRATGGEIVRSFVIALPALALPFVIRAAVV
                     EGIATATEVSTIGIVYGLVVGLLVYRRFDWRRLVPMLVETACLSGAILLIIGTATGMA
                     WGLTQSGFSRALAAGMTGLPGGSAAFIAVSIVAFTVLGSVLEGIPAIVLFGPLLFPIA
                     RAVGVHEVHYAMIIILAMGIGLFAPPFGVGYYAACAIGRVDPAEGIRPIWGYLLALLI
                     GLIVVAVFPWISIGFL"
BASE COUNT          390 a          572 c          535 g          218 t
ORIGIN      
        1 gcgccggcca tcgggaccgc gaaatgcact tcgttcatcg ccgggatgtt cggcagcatc
       61 gccgcgaccg tatcgccgtc ggcgatattt cggcttgcga ggaacgacgc aaaacgccgg
      121 cagcgcgaat aggtctgcga ccaggtgaag gcgcggcctt cgtacacggc gctgagatgg
      181 ctgggataga tggcggcggt gcgcgccagg aagctcagcg gcgtcagcgg aacaaagttg
      241 gcgggcgtct tgtccaaacc gatactgtat tggctttgct taccgttcat cggtcatcct
      301 ccctttgatg ccgcttacag gaaaccgatc gagatccacg gaaaaaccgc cacgacgatc
      361 aacccgatca gcagcgcgag cagatagccc cagatggggc gaatgccctc ggcagggtcg
      421 acacggccga tggcgcaggc ggcataatag cctactccaa acggcggcgc gaacaatccg
      481 atacccatgg cgaggataat gatcatcgca taatggacct cgtggacgcc gaccgcgcgt
      541 gcgatcggaa acagcagcgg gccgaacagc acgatcgccg gaatgccctc gagcacgctg
      601 cccaataccg tgaaagccac gatcgagacc gcgatgaacg ccgcggagcc gccgggcagg
      661 ccggtcatcc cggccgccag cgcgcgcgaa aatccagact gcgtcaatcc ccaggccatg
      721 ccggtggcgg tcccgatgat caggaggatc gcacccgaaa ggcatgccgt ctccaccagc
      781 atcggcacca gccgccgcca gtcgaagcgg cgatagacca gaagaccgac gaccaggcca
      841 tagacgatgc cgatggtgga aacctcggtc gcggtggcga tgccctcgac caccgcggcg
      901 cggatcacga acggcagcgc cagcgcgggc agggcaatga cgaaggaccg gacgatctcg
      961 ccgccggtgg cgcgtttgac gtgactgagg tcttcgccgc ggtagcgcca ccacaccagc
     1021 gccgacaacg tgatcgccag caccacgccg ggcaacaagc cgccggtaaa cagtgccgcg
     1081 atcgaaacgc cggtcaccga accgatcgtg atgagcacga gactgggcgg aatggtctcg
     1141 gtttgcgcgc cggtggccgc gagcagggcg acgagatcgc ccggctttgc gccgcgcgcc
     1201 ttcatttccg gaaacagcac gggtgcgacg gcggccatgt cggcagcctt cgagcctgaa
     1261 atacccgaca ccagatacat cgcgccgacc agcacgtaat gcagaccgcc acggacatgg
     1321 ccgagcaggc ttgccagaaa cgccaccatg gcgcgcgcca tcccggtcat ctcgatcaac
     1381 aggccgagga atacgaacag cggcaccgac agcaggatca ggtggctcat gccctcgtcc
     1441 atgcggccga ccagcactat cagcggcgta tgcgtcgtca gcgccagata gccgtaaatc
     1501 gccaggccaa acccgaaggc aatcggcacg ccggcgaaga cgcagaagcc ggcaacgccg
     1561 acaaagaaga tgatcaaatt gatattgccg agcggccgca gccacggctg cgccagccag
     1621 aacaccgcga tcagcagcgc gaccgacagc accgccccca gcacggtcct gatatcgccg
     1681 gcccgtgcca gccgcaaaaa cgcaaacagc gccat
//
LOCUS       DASWGM010000951         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1621391,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000951 DASWGM010000000
VERSION     DASWGM010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621391"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="VGA15_25625"
     CDS             complement(<1..1577)
                     /locus_tag="VGA15_25625"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX9591107.1"
                     /translation="MRRRQLFAASSAFAMLLSGAALAQTQGPALTGLVSSAEENAMEG
                     VLVSATKEGSTVTFTVVTGKDGRFSFPASKLDPGQYVLRVRAVGYELDNHDAVEIPEQ
                     KAATLDLKLRKTADLAAQLSNGEWLASIPGTDQQKGQLLNCVGCHTLERIVRSKYDSD
                     AFLTQILPRMQGYVNQSIPAHPQLRRAERLMEERGDQRVQVYRALADYLGTINLSAGS
                     PWSYPLKTFARPTGRATRVTYTEYRLPRETISPHDVIADADGIVWYSSFGEQNLGRLD
                     PKTGKVTEYPVPEHKPDFPTGFLGLRADKAGNLWLGNMYQATIVKFDRKAESFQFWPL
                     PPEQNIDAAQVNMASAQSSHIDGKVWAQNNGFAGVHRLNLATGKIETWEPFKNAPKGE
                     PHNIYDIVPDSKNNAYFTDFRQQQIGRVDAVTGEVKLFEIPTARSAPRRGSMDAQDRL
                     WFGEYRGDRIGMFDTRTEQFKEWKLGTCWASPYDVTLDRNEEAWTGSMVTDQVTRLNT
                     GTGEMVDYLLPRSTNIRR"
     gene            complement(1688..2071)
                     /locus_tag="VGA15_25630"
     CDS             complement(1688..2071)
                     /locus_tag="VGA15_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9591108.1"
                     /translation="MIDAIRTTAAAALLMTLLLAGAARADGDAARGEARFQDCAACHR
                     LEAGANNVGPSLHGIFSRKAGELADFRYSPAIKRSGITWTPETLDKFISDPQAVVPAN
                     RMPYAGMVDASDRADLIEYLSKASR"
     gene            complement(2068..>3204)
                     /locus_tag="VGA15_25635"
     CDS             complement(2068..>3204)
                     /locus_tag="VGA15_25635"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX9591109.1"
                     /translation="FPRPSANASQPYTNPDGSKFGGCQYCGYCQRFGCEANAKGSPHM
                     TVIPIAMRNPNFELRTHSWVTKVLKDSDGKRVTGVSYTNVLNGEEFEQPATIVLLCAY
                     AINNVHLMLLSGIGAPYDPVAQKGVIGKNYCYQTGAAATLFFEGKYFNPFMNAGGSNT
                     TIDDFNTNWAFDRAPHDFVGGYNVSGGFNTPLPIGYRPVPSGTPQWGSEWKRATAKWY
                     QTAMVINASGSVMANRFNCYDLDPTYRNAFGQPLMRMTFDYQNNEHKMGRHAAEVIND
                     IAKSMNPTRLNPASARTDPWTVVPYQSTHNTGGTIMGTNPRDSALNKYLQSWDCHNLF
                     VVGANVFPHNSSYNPTGPVGALAYWTADAIKNRYVKNPGPLVQA"
BASE COUNT          534 a          958 c         1030 g          682 t
ORIGIN      
        1 cgccggatgt tggtcgagcg cggcaacaga taatcaacca tctcgccggt tccggtgttc
       61 agacgtgtca cctggtcggt aaccatcgag ccggtccacg cctcctcgtt tctgtcgagg
      121 gtgacatcgt agggcgacgc ccagcacgta ccgagcttcc actccttgaa ctgttcggtc
      181 cgggtgtcga acattccgat gcgatcgccc cggtattcgc cgaaccacag ccggtcctgc
      241 gcgtccatgc tgccgcgtcg cggcgcggag cgagccgtcg gaatttcgaa cagcttgacc
      301 tcgccggtca cggcgtcgac gcgtccgatt tgctgctggc ggaaatcggt gaaataggca
      361 ttatttttcg agtccgggac aatgtcgtag atattgtgcg gttcgccctt cggggcgttc
      421 ttgaacggct cccacgtctc gatcttgccg gtcgcgaggt tgaggcggtg cacgccggca
      481 aagccgttgt tctgcgccca gaccttgccg tcgatatgcg agctttgcgc gcttgccata
      541 ttgacctgcg cggcgtcgat gttctgctct ggcggcagcg gccaaaactg gaatgactct
      601 gccttgcggt cgaatttgac gatcgtcgcc tgatacatgt tgccaagcca caaattgccg
      661 gccttgtcgg cgcgcaggcc gagaaagccg gttggaaagt cgggcttgtg ctcgggaacc
      721 ggatattccg tgacctttcc ggtcttggga tcgagtctgc cgagattctg ttcgccgaag
      781 ctcgaatacc agacgatgcc atccgcatcc gcgatcacat catgcggcga gatcgtctcg
      841 cgcggcagac ggtattccgt gtaggtgacc cgcgtcgccc gaccggtcgg tcgcgcaaac
      901 gtcttgagcg ggtagctcca cggcgaaccg gcgctgagat tgatggtgcc gagataatcc
      961 gcgagcgccc ggtagacctg gacgcgctgg tcgccgcgct cttccatcaa acgctcggcg
     1021 cggcgcaatt gcggatgcgc cggtatgctc tggttcacgt atccctgcat gcgcggcagg
     1081 atctgcgtga gaaaggcatc ggagtcatat ttcgaacgca cgatgcgctc gagcgtgtgg
     1141 cagcccacgc agttgagcaa ctggcccttt tgctgatcgg tgccgggaat gctcgccagc
     1201 cattcgccat tggacaattg cgcggcgagg tctgcggtct tgcgcagctt gaggtccagc
     1261 gtcgcggctt tctgctccgg gatttcgacg gcgtcgtgat tgtccaactc gtagccgacc
     1321 gcgcgcacgc gcaggacata ttgacctgga tccaacttgc tcgccgggaa gctgaagcgg
     1381 ccatccttgc cggtcactac cgtgaacgtg acggtcgagc cttccttggt ggcgctcacc
     1441 aacaccccct ccatggcgtt ttcctcggcg gaactgacga ggccggtcag tgcggggcct
     1501 tgcgtttgcg ccagggcggc gcccgaaagc agcatggcga atgccgaact ggcggcgaaa
     1561 agctgacgac ggcgcatggg ttcctcccgg atgacgttgt tctttttgct tctagccggt
     1621 acaggccctg cggcctgcac gtcgcaaaca gttgtctgcg cgccaagcgg tcttccgttc
     1681 ggaccgacta cctcgatgcc ttcgatagat attcgatgag atcggcgcgg tcgctcgcat
     1741 cgaccatgcc ggcgtagggc atgcggttgg ccggcaccac ggcttgcgga tcgctgatga
     1801 acttgtcgag tgtctccggc gtccaggtta tcccactacg tttgatcgcc ggcgagtagc
     1861 ggaaatcggc gagctcgccg gccttgcgcg aaaaaatgcc gtgcaggctt ggccctacat
     1921 tgttggcgcc ggcctccaat ctgtggcagg ccgcgcaatc ctggaagcgc gcttcgccgc
     1981 gcgcggcatc gccgtccgcc cgggccgcgc ctgccagcag caaggtcatc aacagcgctg
     2041 ctgccgccgt cgtgcgtatc gcgtcgatca tgcctgcacc agcgggccag gatttttgac
     2101 gtaacggttc ttgatggcgt ccgcggtcca gtaggcaagg gcgcccactg gaccggtcgg
     2161 gttgtacgat gaattgtgcg ggaacacgtt ggcgccgacc acgaacagat tgtggcaatc
     2221 ccaactctgc agatatttgt tgagcgcgct gtcgcgcgga ttggtgccca tgatggtgcc
     2281 gccggtattg tgcgtgcttt ggtagggcac cacggtccaa ggatcggtgc gcgcggatgc
     2341 ggggttgagc cgcgtgggat tcatcgattt cgcgatgtcg ttgatcacct ctgcggcgtg
     2401 gcggcccatc ttgtgttcgt tgttctggta atcgaacgtc atgcgcatca gcggctgacc
     2461 gaacgcgttg cgataggtcg gatcgaggtc gtagcaattg aagcggttgg ccatgacgct
     2521 gccgctggca ttgatcacca tcgcggtctg gtaccacttc gccgtcgccc gcttccattc
     2581 gctgccccat tgcggcgtgc ccgatggcac cgggcggtag ccgatcggca gcggggtatt
     2641 gaagccgccc gacacgttat agccgccgac gaaatcatgc ggcgcgcggt cgaacgccca
     2701 gttggtgttg aagtcgtcga tggtggtgtt ggagccgccg gcgttcatga acggattgaa
     2761 atattttccc tcgaagaaca gcgtcgcggc ggcgccggtt tgatagcaat aattcttgcc
     2821 gatcacgccc ttctgcgcga ccggatcgta gggcgcgccg atgcccgaca gcagcatcag
     2881 atgcacattg ttgatggcat aggcgcatag cagcacgatg gttgccggct gctcgaattc
     2941 ctcgccgttg agcacgttgg tgtagctgac gccggtgacg cgtttgccgt cggaatcctt
     3001 gagcaccttg gtgacccagg aatgcgtgcg cagctcaaaa ttcggattgc gcatggcgat
     3061 cgggatcacc gtcatgtgcg ggctgccctt ggcgttggct tcgcagccga agcgctggca
     3121 atagccgcaa tactggcagc cgccgaattt cgaaccgtcc gggttggtgt agggctgcga
     3181 ggcattcgcc gacggccgcg ggaa
//
LOCUS       DASWGM010000952         5527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_396249,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000952 DASWGM010000000
VERSION     DASWGM010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396249"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VGA15_25640"
     CDS             <1..132
                     /locus_tag="VGA15_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591110.1"
                     /translation="AAKVAKSAHARGTTLKEEAVRLGFVSAAEFDRLVQPAKMTHPG"
     gene            520..708
                     /locus_tag="VGA15_25645"
     CDS             520..708
                     /locus_tag="VGA15_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEX9591111.1"
                     /translation="MGDVINLRRFKKRAEKQQSAKQAEANRTRFGRTKSERTLDQQRS
                     SRASDLLDQHRIDGEDAS"
     gene            705..965
                     /locus_tag="VGA15_25650"
     CDS             705..965
                     /locus_tag="VGA15_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX9591112.1"
                     /translation="MKSPVVKRSIVVAGHKTSVSLEEAFWNGMKEISGLRGMTLSELV
                     GEIDSNRQQGNLSSAIRLFVLDYFRTRAVSAAPDAKSAQVSH"
     gene            complement(962..1180)
                     /locus_tag="VGA15_25655"
     CDS             complement(962..1180)
                     /locus_tag="VGA15_25655"
                     /inference="COORDINATES: protein motif:HMM:NF012663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591113.1"
                     /translation="MAGFDTIIRHGTIATASETYKADVGIKEGRIVAIGEKLADADEV
                     VEAAASMSDRCGSVPSIKFEDAVSPNTR"
     gene            complement(1213..1941)
                     /locus_tag="VGA15_25660"
     CDS             complement(1213..1941)
                     /locus_tag="VGA15_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu/hydantoin racemase"
                     /protein_id="HEX9591114.1"
                     /translation="MTKRILLGMLTPSSNTALEPITTAMVAGLPEVSAHFSRFKVTEI
                     ALSNKALAQFDNDEILRAAELLAHAKVDSIGWNGTSSGWLGFEADIRLCEQITAATGI
                     QATTSMLALNEILERKKVTRLGYVTPYLDDVQAKILENYARLGIACRGERHLGLQDNF
                     SFSEVPVAQLENMTREVAQDRPDAITIICTNLRAAPSVATLEREIGIPIYDTIATVVW
                     KCLKLAGVDTRRVVDWGSLFQQVA"
     gene            complement(2116..>5527)
                     /locus_tag="VGA15_25665"
     CDS             complement(2116..>5527)
                     /locus_tag="VGA15_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX9591115.1"
                     /translation="SLGSLMRGEWRATELTINGMALDLGLDSQGRIDWPASTGKFNLG
                     SLAIDRLNLTGRIALHDASSRGTLELNDIAFSGDVRSLAGSVRGDGTFTLTGVRYPFR
                     VSSGQSADASGTRVHLSIDPGERALSADLDGVLNFEARAPRFEGAVTLAAPHGAKPRG
                     NGGEASQTPWRISARIKADPSAARLEQLEASFGSEETALKFTGFADIRLGGLPLLHAA
                     LSARQLDADKFAAKDNTTAEPIRLLPGFRALMAAMPSVPFPAQIEFSSEQVMLGGRPV
                     QNVAADLHADARSWTIDRLDLRAPGATSVSLSGTSAKVGPSSDFTGALSVDSSDPDAL
                     MAWLLGRSEVTHRSQKPLRLSGDVSVTSSSVAIDAMKAEIDGGAVEGRLAASSQAPSG
                     SRFYADLKAERLDLDAAAAFVRSLAGSPTEWPDEAQVSLDIGRATSAGQELRPFMTKL
                     GYGPKTISLDQLKVGEPHGVMMEGTGSFDRVNATGRLALNSSAASLGQITGLIAPLAP
                     ALASRLGAMEQGPGPTRLKLVLDLDKNAEHADRADARAVFDLDAPQLKGAVTITATPA
                     IAAVRGIDLDALQHSEVGIESKLTSARGGSLLVLLGLDRTIAVGEGPLEFQGSATGIW
                     HAPLLLNVKITGAGLDAEAQGIAEPWAQEPKAGVSLKVRSVNLAPLFDLKPSDALAQN
                     ISLSSHVSLAGSRLILDDIDTAMSGSRLRGRLALTLDEPRTVAGEVGLDALDLAPAFA
                     LAIGAAGHDAAEPLGAGLVKGWRGRIAFQALRGSLPGGGELRPVSGTVKSDGQSLTFD
                     AISGGLGGGEATANLDAKQSANGIALNARVQLSGVDGTVLRYRGLAMPAGRASMQMTL
                     ASQGRSASALAGALSGSGTLALESARVAGLDPRAFDAAIRASDNGQATDDIRLRQIVE
                     PALSAGALSVGSAQIPFNIRDGRIRVGATTLDAEGARAVVSGGYDIPADQADLRVAIA
                     STTAGAATSRPEIQLFVVGSPDALTRTVDVAALSSWLAVRAIDRETRRLDSIERGDPT
                     AALPASIAPSAPAQPSTGASDVDLSGMPLSEVPAFGRDPRRPPSRPRIGVPHAPLAPS
                     NAPVAAQQLAPLPPPIEVRPTPSIVRQPKQRPPLALTPPAAAPPRPTF"
BASE COUNT         1034 a         1746 c         1741 g         1006 t
ORIGIN      
        1 gccgccaagg tcgccaagtc agctcacgcc cgcggaacca cgttgaagga agaggctgtg
       61 cggctgggct ttgtctcggc ggcggaattc gatcggctgg tgcagcccgc caaaatgaca
      121 catccgggct gatgccgcgg cgcaatcccc gtagaactac ggggtcgaaa attagttacg
      181 agtcatataa cgcctaaaga ataataccgt taagctatcg ccgtcgcgca ggcccgtgct
      241 agagcacgaa aatatttcgt cttcacaaaa gcggaacatt tttctgtcgc tatcaaggtg
      301 ggggatttat cgaatgagga ctgagccggc tggcgcgtat ttcgcaagtg cgaataccag
      361 ctttgcaact gcaatacgcg taaggatatt gatcgagagc attttcctga cgctgaccgg
      421 attgcgcttg gccggaactt gctctcacac cccgcgcggc ctctcctgcc ttcgtgaaga
      481 tgccgcatgt caccagctaa ttgcatgacg ggggcgagca tgggggacgt aatcaacctg
      541 aggcgattta agaagcgcgc cgaaaaacaa caatcggcga aacaggcgga ggccaaccga
      601 acacggtttg gccgaaccaa atccgaacgc acgctggacc aacagcgttc aagtcgggca
      661 agcgatttac tggatcaaca tcggatcgac ggcgaggacg cgtcatgaaa tcgcccgtgg
      721 tgaaacgctc gatcgtggtt gcgggtcaca agaccagcgt cagccttgaa gaggcattct
      781 ggaacggcat gaaggagatc tcgggccttc gtggcatgac gctgtccgaa ctggtgggtg
      841 agatcgacag caaccgccag caaggcaatc tgtcttcggc catccgcctc ttcgtgcttg
      901 attattttcg aacgcgcgcg gtgtccgccg cgccggatgc aaaatcggcg caggtgtctc
      961 actaacgcgt gttcggagat accgcgtcct cgaacttgat cgaggggacg gacccgcaac
     1021 gatcgctcat cgatgcggcg gcttcgacga cttcgtctgc gtcggccaac ttttcgccga
     1081 tggcgacaat gcgcccttcc ttgatcccga catcagcctt gtaggtttcg cttgcggtgg
     1141 cgatcgtacc gtgacgaatg atcgtgtcga acccagccat ggtcgatatc tccagcaaga
     1201 attcggatgc gatcacgcga cctgttgaaa cagcgaaccc caatcgacga cgcgcctggt
     1261 atcgacgccg gcaagtttga ggcatttcca gaccacggtg gcaatggtgt catagattgg
     1321 aatgccgatc tcgcgctcca gcgtcgcaac cgatggtgcg gccctcaaat tggtgcagat
     1381 gatcgtgatc gcatccggtc tgtcctgcgc cacctcgcgg gtcatattct ccagctgcgc
     1441 gaccggcact tcggaaaagg aaaaattgtc ttgcaggccg aggtgccgct cgccccggca
     1501 ggcaatgccg agccttgcat agttttccag aatttttgcc tgcacatcgt cgagataggg
     1561 cgtgacgtag ccgagccgtg tcactttctt ccgttcgagg atttcgttga gggccagcat
     1621 cgaagtcgtc gcttgtatcc cggttgcggc ggtgatttgt tcgcacaatc gaatatccgc
     1681 ttcaaagccc agccagccgg aggatgtacc gttccatcca atcgagtcga cctttgcatg
     1741 cgccaataat tccgcggcac gcaggatctc gtcattgtcg aattgcgcca gcgccttatt
     1801 ggaaagggcg atttcggtta ccttgaagcg tgaaaagtgc gccgacactt ccggcaaccc
     1861 ggcaaccatc gcggtcgtga tcggctccag cgccgtattc gacgacggcg tcaacattcc
     1921 aagaagaata cgctttgtca ttggtgatcc atggcctccc gggttgaatt cgcccgctcc
     1981 gagcgcagca aatccagaat gtgtcgtttg gtgggtgaga acaatgcctg atttcaacat
     2041 ctgttcgggg cgcagtcgcg cgcggatcca tcaagctcgc gtgcattctg gctacgagta
     2101 tggggtcgcg gttctctaga acgtcggtcg cggtggcgcg gcagccggcg gcgtcagcgc
     2161 caacggcggc cgctgcttcg gctgccgtac aatgcttggc gtcgggcgga cctcgatcgg
     2221 cggcggcagt ggcgcgagct gctgggcagc aaccggcgca tttgatggag cgagcggcgc
     2281 atgcggcacg ccgatcctgg gcctcgacgg cggccgccgc ggatcgcgac cgaacgcggg
     2341 aacctcggaa agcggcatgc cggacaggtc cacgtccgac gctccggtcg aaggttgcgc
     2401 gggcgcggat ggtgcaatcg acgccggcaa cgccgctgtc ggatctccgc gctcgatcga
     2461 atccagccgg cgggtttcgc gatcgatcgc cctcaccgcc agccatgatg atagcgcggc
     2521 aacgtcgacc gtgcgggtga gagcgtcggg cgagccgaca acgaacagct gaatttccgg
     2581 acgactggtg gcggctccgg ccgtcgtcga agcgatggcg acgcggagat cggcctggtc
     2641 ggcgggaata tcgtatcccc ccgatacgac ggcgcgggcg ccctcggcat cgagcgtggt
     2701 cgcgccgacg cgaatccggc catccctgat gttgaatgga atttgcgccg atccgaccga
     2761 caacgcgcca gcggacaagg ccggctcgac gatctgccgc agcctgatgt cgtccgtcgc
     2821 ctgcccgttg tcgctggcgc ggatcgcggc gtcgaacgcg cgcggatcga gcccggcgac
     2881 gcgagcggat tccagcgcca gggttccact tccggacagc gcgcctgcca gcgccgacgc
     2941 gctgcggccc tggcttgcca acgtcatctg catggacgcg cgacctgcgg gcatcgcaag
     3001 gccgcgatag cgcaaaaccg tgccgtcgac accgctgagc tgaacgcgcg cgttcaacgc
     3061 aattccattc gcggactgct tcgcgtcgag attggcggtc gcctcaccgc cgcctagtcc
     3121 accgctgatc gcgtcgaagg tcagcgattg gccatcgctc ttgacggtac cgctgacggg
     3181 gcgcaactcg ccgccgcccg gaagcgagcc gcgcaacgcc tgaaacgcga tacgaccgcg
     3241 ccagcctttc accagtccgg ctcccaacgg ctcggccgca tcatgccccg cggcgccgat
     3301 ggcgagcgca aatgccggcg ccagatcgag cgcatcgagg ccgacttcgc ccgccacggt
     3361 ccttggctcg tcaagggtca acgccagacg cccgcgcagt cgcgaacccg acatcgcggt
     3421 gtcgatatcg tccaggatca gcctgctgcc cgccagcgaa acgtgcgagg aaaggctgat
     3481 gttttgcgcc agcgcgtcgg agggtttgag atcgaacagc ggagcgagat tgacgctgcg
     3541 aacttttaga ctgacgccgg ccttgggctc ctgcgcccat ggttcggcga taccttgcgc
     3601 ttcggcatcg aggccagctc ccgtgatctt cacgttcagc agcaacggcg catgccagat
     3661 gccggtcgcc gacccctgaa attccagcgg gccttcgccc acggcgatgg tgcgatcgag
     3721 ccccagcaag acaagcaatg aaccgccgcg cgccgaggtt agtttcgatt caatgccgac
     3781 ttcgctgtgc tggagcgcat cgagatcgat gccgcgcacg gccgcgatcg cgggcgttgc
     3841 cgtgatcgtg acggcgccct tgagctgcgg cgcgtcgaga tcgaaaacgg cgcgcgcgtc
     3901 ggcgcgatcg gcatgttcgg cgttcttgtc gagatcgagc accagtttaa gtcgcgtggg
     3961 gcctgggccc tgttccatcg cacctagccg cgacgccagt gccggcgcga gcggcgcgat
     4021 cagcccggtg atttgaccaa gcgacgcggc gcttgaattc agcgccaatc gtccggtggc
     4081 attgacgcgg tcgaaactcc ccgtgccttc catcatcacg ccatgaggct cgccgacttt
     4141 caattgatcc agcgagatgg ttttcggacc gtagccgagc ttggtcatga acggtcgcaa
     4201 ttcctggccg gccgaagtgg cgcggccgat atccagcgat acctgcgctt cgtcaggcca
     4261 ctcggttggc gagccggcca gcgaacgcac gaatgccgca gcggcatcga gatcaagacg
     4321 ctccgccttc aaatccgcgt agaaccgcga gccgctcggc gcctgacttg aggccgccag
     4381 ccgcccttca acggcgccgc catcgatctc cgccttcatg gcgtcgatgg caacgctact
     4441 cgaggtcacg ctgacatcgc cgctcaggcg caacggcttc tggctgcgat gcgtgacctc
     4501 actgcgaccc agcagccacg ccatcagcgc gtccggatcc gaggagtcga cgctgagcgc
     4561 tccggtgaaa tcgctggacg ggccgacctt cgcgctggtg ccgctcaagc tcacgctggt
     4621 cgcgcccggc gcgcgaagat ccagccgatc gatggtccat gatctggcgt ccgcgtgcag
     4681 atcggccgca acattctgca ccggacggcc gcccagcatg acctgttcgg agctgaattc
     4741 gatttgcgcc ggaaaaggaa ctgaaggcat cgccgccatc agagcgcgga atcccggcaa
     4801 cagtcggatc ggctcggcgg tggtgttgtc cttggcggcg aacttgtcgg cgtcgagctg
     4861 gcgcgcggag agcgcagcat gcagcagcgg caatcctcct aaccgaatgt ccgcaaatcc
     4921 tgtgaatttc agtgccgtct cttcggagcc gaagctggcc tcgagctgtt cgagccgcgc
     4981 ggctgaagga tcggccttga tcctggccga gatccgccac ggcgtctgcg acgcctcgcc
     5041 gccgttgcct ctcggtttgg ctccgtgggg agcggccagc gtcaccgcac cttcgaagcg
     5101 cggtgcgcgc gcctcgaagt tgagcacgcc gtcgagatcg gcggacaatg cacgctcgcc
     5161 gggatcgatg ctgagatgga cgcgggtgcc gctggcatcg gcgctctgac cggatgagac
     5221 ccgaaacggg taacgcaccc cagtcagcgt gaaggtgccg tcgccccgaa ccgatccggc
     5281 cagcgagcgc acgtctccgc tgaaggcgat atcgttgagc tcgagcgtgc cgcggcttga
     5341 ggcgtcatgc agcgcgatgc ggccggtcag gttcaagcgg tcgatcgcaa gcgatcccag
     5401 attgaacttt ccggtcgacg ccggccaatc gatccggcct tgcgaatcga gaccaagatc
     5461 gagcgccatg ccgttgatcg tcagttcggt ggcgcgccat tcgccgcgca tcagcgagcc
     5521 caggctg
//
LOCUS       DASWGM010000953         7254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_236731,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000953 DASWGM010000000
VERSION     DASWGM010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236731"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..913
                     /locus_tag="VGA15_25670"
     CDS             5..913
                     /locus_tag="VGA15_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9591116.1"
                     /translation="MAAFTLAHLSDPHLAPLPAARLRDLAGKRAFGYLNWTRNRHKFH
                     RRDVLDALVSDMQAQAPDHIAITGDLVNLALEAEFAPSRAWLESIGAPDRVTVIPGNH
                     DAYVRATRHHFAEAWGHYFDGDDRRDGGITFPSLRRRGPLALISVSSAVPTPPLMATG
                     RLGRTQLDKLEGVLAGLSPEQAFRVLLIHHPLHSDSRVKRLTDSSKLLALLKQHGVEL
                     ILHGHDHVHSTIWIDGPTGAIPAIGVPSASAVAHGRYPGAAYNLFSIERDGAAWRCEQ
                     TIRGIDDKLRVHELRRTTLDVAQGRE"
     gene            complement(887..1441)
                     /locus_tag="VGA15_25675"
     CDS             complement(887..1441)
                     /locus_tag="VGA15_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591117.1"
                     /translation="MSDAQNTGSNAVSDTRPIRLRDALRQARIEAADRTGVVVDLRDA
                     EVARLEILNEALDPLFAQIPEQVDLFDRGISEGDTPRLWIDVVAHVLMGRDKRIYRFV
                     QDTRFGRIVIAESHDVAAIVDAVTDYVARRMVEREHALVATPAKPLVLEKSRRHGFWI
                     FALGFVVGAVALFALALFAALRNI"
     gene            complement(1652..1861)
                     /locus_tag="VGA15_25680"
     CDS             complement(1652..1861)
                     /locus_tag="VGA15_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591118.1"
                     /translation="MTKGSNFWVIGGEFGSMNFHTLVEGSAQVRGPFKTRKEAEDCWR
                     EVSEENRHKAGVRFSIVEEPSRVPA"
     gene            complement(1875..3182)
                     /gene="aceA"
                     /locus_tag="VGA15_25685"
     CDS             complement(1875..3182)
                     /gene="aceA"
                     /locus_tag="VGA15_25685"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX9591119.1"
                     /translation="MAKTFEQLVPAPAGRFDGISRPYTPAEVEKLRGSVPIAYTLAEK
                     GANRLWNSLKTEPFVNSLGAVTGNQAMQQARAGLPAIYLSGWQVAADANTAGAMYPDQ
                     SLYPANAGPELCRRINRTFQRADQIEHSEGGAKMDWFVPIVADAEAGFGGPLNSFEIM
                     KAYIEAGAAGVHFEDQLASEKKCGHMGGKVLIPTAAHERNLIAARLAADVCGTPTFVL
                     ARTDAESAKLITADVDERDREFITGERTAEGFFRLKPGTGLAHCIKRGIAFAKYADLL
                     WWETSTPNLEEAREFAEAVHKVYPGKMLAYNCSPSFNWEANIDKATIAKFQREIGAMG
                     YKFQFVTLAGFHSLNHGMFELARGYKAEGMAAYSRLQQAEFASEKHGYSATRHQREVG
                     TGYFDLVSTTITGGTSSTTALHDSTEAAQFKTHGDEIKVQAAE"
     gene            3455..4900
                     /locus_tag="VGA15_25690"
     CDS             3455..4900
                     /locus_tag="VGA15_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX9591120.1"
                     /translation="MAAESGKKLFVGPRFRRIRRQLGLSQTQIAEGLGISPSYINLIE
                     RNQRPVTAQILLRLAEAYDLDLRDLATADEDRFFAELNEIFSDPLFRQIDLPKQELRD
                     LAELCPGVTHSLQRLYAAYTEARRGETLVAAQMADRDEGARFEANPIERVRDLIEANR
                     NYFPELEQAAENLRDELNVPSEQLFAALSTRLREKHSIITRIMPVDVMRETLRRFDRH
                     RRQLLISELVDGPGRAFQLAFQMGLAECSASFETIAGRAGPLDDTPRRLYRITLANYF
                     AAAVMMPYQVFHGAAETLSYDVHVLARRFNAGFEQVCHRLTTLQRPNARGIPFFLLRV
                     DNAGNVSKRFSSGTFPFSKFGGTCPLWNVHSTFDTPDRLLSQVIELPDGTRYFSIAQM
                     VRRPVAPHPQAQPRFAIGLGCEIRHASRLIYASGMDLEKTEGTPIGVNCRLCERENCS
                     QRAEPPITRTLILDENTRRVSSFAFSNAREL"
     gene            complement(6009..6812)
                     /locus_tag="VGA15_25695"
     CDS             complement(6009..6812)
                     /locus_tag="VGA15_25695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591121.1"
                     /translation="MEMPSDPKANTRVRIAFLLLFVFMVVFSVGAYFVADLAKLQESI
                     AARESQTALQGITDPRQIDEALKQHPSNKFLQMIAMVTKAANETNAATEKLSTEVEPP
                     AISKNINLGAASRSDLEALRRDLNTAQANATTLMPRCIALLGTERDNVEKYALSLHVG
                     KDTISRFLDNIDKRHAETTAFTSKTLLARAEYYRAYEKYVAVLVGEFGAYKVANGQFI
                     FPLQRTAERYNVAANAMTAAAKRVAELAEERKTLMQSQLEGWEQFVSGK"
BASE COUNT         1355 a         2257 c         2180 g         1462 t
ORIGIN      
        1 ggtgatggcg gcattcacgc tggcgcacct gtccgatcct cacctggctc cgctgcccgc
       61 ggcgcggctg cgcgacctcg ctggcaagcg cgccttcggc tatctcaatt ggacccgcaa
      121 ccgccacaaa ttccatcgcc gcgacgtgct cgacgcactt gtgtcggaca tgcaggcgca
      181 agcgccggat cacatcgcaa tcaccggcga tctcgtcaac ctcgcgctgg aagcagagtt
      241 tgcgccctcg cgagcctggc tggagagcat cggcgcgccg gatcgggtca ccgtgatccc
      301 cggcaatcac gacgcctatg ttcgtgccac ccggcatcac ttcgccgagg cctggggaca
      361 ctattttgat ggcgacgata ggcgcgatgg cggcatcacg tttccatcgc tgcgccggcg
      421 cggaccgctg gcgttgatca gcgtgtcgtc ggcggtcccg acgcccccct tgatggcgac
      481 gggacggctt gggcgcaccc agcttgataa gctcgaaggc gtgctggcgg gactatctcc
      541 ggagcaggcg tttcgtgtgc tgctgattca tcacccgctg cattcggatt cgcgcgtcaa
      601 gcggctgacc gattcatcca aattgctcgc gctgctgaag caacacggcg tggaattgat
      661 cctgcacggg catgaccacg ttcattcgac gatatggatc gacgggccga cgggcgcgat
      721 acccgccatt ggcgtgccct cggcatcggc ggtcgcgcat ggccgctatc cgggagcggc
      781 gtacaatctg ttttcaattg agcgcgatgg cgccgcgtgg cggtgcgagc agacgatacg
      841 gggtatcgac gataagttgc gcgttcatga attgcggcgg acgacgctag atgttgcgca
      901 aggccgcgaa tagcgcgagg gcgaacagcg cgaccgcgcc gaccacgaag ccgagtgcaa
      961 agatccagaa gccatggcgg cgcgacttct caagcacgag cggcttcgcc ggcgtcgcca
     1021 ccagcgcgtg ctcgcgctcg accatgcggc gggcgacata atcggtgacg gcgtcgacga
     1081 tcgcggcgac gtcgtgggat tccgcgatca cgatgcggcc gaaccgggta tcctggacaa
     1141 aacggtagat gcgcttgtcg cgtcccatca ggacatgcgc aaccacgtcg atccatagcc
     1201 gcggcgtgtc gccctcgctg atgccgcgat cgaacagatc gacctgttcc gggatctgcg
     1261 cgaatagcgg gtcgagcgcc tcgttcaaaa tttcgagccg cgccacctcg gcgtcacgca
     1321 ggtccacgac gacaccggtg cgatcggcag cctcgatccg cgcctgccgc agcgcatcgc
     1381 gcaacctgat cggacgcgta tcgctgacgg cattgctccc ggtgttttgc gcgtctgaca
     1441 tccgctggcg gcctttgtcc tgatgggtga ggattgccct taacctatca gtaaccgtgc
     1501 tgtccgcaaa ggccgtttgt tcccaagggt ttacgtgttg actcgcctgc tcactcgccg
     1561 agagagcccc tcacccctgc cctctcccca cgaagggcgg ggagagggag aagagaggtg
     1621 accgcaagcg ggagaggagg aagaggcgcc gtcaggccgg cacgcgcgag ggctcttcga
     1681 cgatcgaaaa gcgcacgccg gccttgtggc ggttttcttc cgaaacctcg cgccagcaat
     1741 cttcggcctc cttgcgggtc ttgaacgggc ctctgacctg ggccgagcct tccacgagcg
     1801 tgtggaagtt catcgagccg aactcgccgc caatcaccca gaaattgctg ccctttgtca
     1861 tcatagcctc cctactattc agccgcttga accttgattt cgtcgccgtg cgtcttgaac
     1921 tgcgcggctt cggtcgagtc gtgcagcgcg gtggtcgacg aggtgccgcc ggtgatagtg
     1981 gtcgacacca gatcgaagta gccggtgccg acttcgcgct gatggcgcgt cgccgaatag
     2041 ccgtgcttct cggaagcgaa ctcggcctgc tgcaggcggg agtaagccgc catgccttcg
     2101 gccttgtagc cgcgcgccag ctcgaacatg ccgtggttga gcgagtggaa acccgccagc
     2161 gtgacaaact ggaacttgta gcccatcgcg ccgatctcgc gctggaattt tgcaatcgtc
     2221 gccttgtcga tgttggcttc ccagttgaac gacggcgaac aattgtaggc cagcatcttg
     2281 ccgggataga ccttgtgcac ggcttccgca aactcccttg cctcttcgag gttcggggtc
     2341 gaggtctccc accacagcaa gtcagcgtat tttgcgaacg cgatgccgcg cttgatgcaa
     2401 tgagccaagc ccgtgccggg cttgaggcgg aagaaacctt ccgcggtgcg ctcgccggtg
     2461 atgaactcgc gatcgcgctc gtccacgtca gccgtgatca gcttggcgct ctccgcgtcg
     2521 gtgcgcgcca ggacaaaagt cggcgtgccg cagacgtcgg ccgcaagacg cgccgcgatc
     2581 aggttgcgct catgcgccgc ggtcgggatc agcaccttgc cgcccatgtg gccgcacttc
     2641 ttttccgacg cgagctggtc ctcgaagtga acgccggccg cgcccgcctc gatataggcc
     2701 ttcatgatct cgaacgagtt caaggggccg ccgaagcctg cttccgcgtc ggcgacgatc
     2761 ggcacgaacc agtccatctt ggcgccgcct tcggagtgct cgatctggtc ggcacgctga
     2821 aaagtgcggt tgatgcggcg gcaaagctcc gggccggcat tggccggata aaggctctgg
     2881 tcgggataca tcgcgcccgc ggtgttggca tcggctgcga cctgccagcc cgacagatag
     2941 atcgcaggca agccggcgcg cgcctgctgc atcgcctggt tgccggtaac ggcgcccagc
     3001 gaattgacaa acggctcggt cttcagcgaa ttccaaagcc gattggcacc cttctcggcc
     3061 agcgtgtagg cgatcggcac ggagccgcgc agcttctcca cctcggccgg ggtgtaggga
     3121 cggctgatgc catcgaaacg ccctgcgggc gcgggaacca gctgttcaaa ggtcttcgcc
     3181 atcgtaatct ccaatcgaca ttcgtgacag tgcattgcga aacgtgtgtc gagagctaaa
     3241 cgctaaactt tttaattcgt acagaccgct tgcatcctaa tcgtgatgtc atgtaacata
     3301 tgaacatgta atagatgtca atttgtcatt tttgtcacaa acaagagctc agtgacgtca
     3361 ttccggggcg tgcgaaggac gaacccggaa tctcgagatt ccggatcgcc gctccgcgcc
     3421 gtccggaatg actacaaggg tcggtgagac tgccatggcc gcagaatccg ggaagaagct
     3481 gtttgtcggt ccgcgcttcc ggcgcatccg ccggcaattg ggattgtcgc agacccagat
     3541 cgccgagggg ctcggcattt ctccgagcta tatcaacctg atcgagcgca accagcgccc
     3601 ggtgacggcg cagatcctgc tgcggctggc ggaagcctat gacctcgatt tgcgtgatct
     3661 tgcgaccgcc gacgaggacc ggtttttcgc cgagctgaac gagatctttt ccgatccgct
     3721 gttccggcag atcgacctgc cgaaacagga attgcgggat ctcgccgaat tgtgccccgg
     3781 tgtgactcac tcgttgcaaa ggctctacgc cgcctacacc gaagcccgcc gcggcgagac
     3841 cctggttgcg gcgcaaatgg ccgaccgcga cgagggcgct cgcttcgagg ccaatccgat
     3901 cgagcgcgtc cgcgacctga tcgaggccaa ccgcaattat ttccctgaac tcgaacaggc
     3961 ggcggaaaat ctgcgcgacg aattgaacgt gccgtccgag caactgtttg cggcactcag
     4021 cacgcggttg cgcgaaaagc attcgatcat cacccgcatc atgccggtcg atgtcatgcg
     4081 cgagacgttg cggcgcttcg atcgccatcg ccggcaattg ctgatctccg aactggtcga
     4141 tggccccggc cgcgcgttcc agctcgcctt ccagatgggg cttgccgaat gcagcgccag
     4201 ttttgagacc atcgcgggtc gcgccgggcc gctcgatgac accccgcgcc ggctctatcg
     4261 gattacgctg gcgaattatt tcgcggccgc cgtgatgatg ccctatcagg tgtttcatgg
     4321 tgcggcggaa accctgagct acgatgtcca cgtgctggcg cggcgtttca atgccggttt
     4381 cgagcaggtc tgccatcgcc tgaccacgct gcagcggccc aacgcgcgcg gcattccgtt
     4441 cttcctgctg cgcgtcgaca acgccggcaa tgtttccaag cgattctctt ccggcacctt
     4501 cccgttttcg aaattcggcg gcacctgccc gttgtggaac gtgcactcga ccttcgacac
     4561 gccggaccgc ctgctcagcc aagtgatcga actgcccgac ggcaccaggt atttctcgat
     4621 cgcgcagatg gtgcgccggc cggtggcgcc gcatccgcaa gcccaaccgc gctttgcgat
     4681 cgggttgggg tgcgaaatcc gccacgcctc gcggctgatc tacgccagcg ggatggatct
     4741 ggaaaaaacc gagggcacgc cgatcggcgt caattgccgg ctttgcgagc gcgaaaactg
     4801 cagccagcgc gccgagccgc cgatcacgcg gacactgatc ctggatgaga acacgcggcg
     4861 ggtgtcgtcg ttcgcgttca gcaatgcgag ggagctgtga gtaaggcctc gcagccgtca
     4921 ttccgggatg gtccgcagga ccagacccgg aatctcgaga ttccgggttc gatgcggagc
     4981 ctgtcatcgg gccgcgcgtt gcgcggaccc gttggcatcg ccccggaatg acggtgtctg
     5041 gattgcttgg tcgcttcgct cctcgcaaaa ccgtcatact ccgcgaaagc ggggtatcca
     5101 gtacgccgca gcttttcgat tccatcattg gcgcctcgga atactggatc gcccgccttc
     5161 gcgggcgatg acggctgaga gtgttggcgc atttgtattc atcaaatacg gcttcgcgat
     5221 cccgcggcgc gatgcgcccg agtcgtgcat gaaatcttcc gcccccttga gggcgtgggg
     5281 aatgccgggt gcccgctgca cccgcggcct cgtgtgcact ttgtattggt agaacgcaca
     5341 cgagtaacaa cgagtacacc ggaatcgccc ggcattcccg cacgcaatgg ttttacggct
     5401 tatgtcgtgc tctccccggt gaccgggctc ttttgccacc gtcgcctgcg gatatgtttt
     5461 gtctaagccc ggtagggccg acgcaactcc gccaacttga cgccagcgtc ggggcgtcag
     5521 gaccacacga ttttgccgtc cgctgcaaca tctctcgtcc gctcgcttgg tgatcgctca
     5581 caggcctcat cgacccgccc tgcgatcccg tcgcgcgcaa gacgctgccg cgtccaccgc
     5641 atccgcaccc cgcgtctgtg acgatcgcga tacgcccctc tggtggggtg ggatggcgcg
     5701 aatttgtaga gatgatttgc ccgacgggtg aagcgaaata tttttgcaaa ggggactgga
     5761 ctacccaaat cacattgatc cgtttcaaca aattcgccgg tgcgcgcaag acggattagc
     5821 ctgacgggtc cgcaaggcgc cgcccgatga caggctcagc gcaatccaga agctgcgatg
     5881 gcagagaaat ggattgcttc gtcgtcattg ctcgcaatga cgagaagata cgcagcctgg
     5941 atgggcgaag cggcatccga gaacgaacgc gacgccgggg ctcgaccgta tggcgcgagt
     6001 cggttcagtc atttaccgct gacgaactgc tcccaaccct ccagctgcga ctgcatcagt
     6061 gtttttcttt cttctgccaa ttcagccaca cgctttgctg cagctgtcat tgcattggcc
     6121 gcgacattgt aacgttccgc ggtacgctga agtggaaata tgaattggcc atttgccacc
     6181 ttgtaggcac cgaattcccc gaccagaacc gcgacatatt tttcgtaagc gcgataatat
     6241 tcagcgcgcg ccaataacgt cttggaagta aaagccgtgg tttccgcgtg ccgcttatcg
     6301 atgttatcca ggaacctgct gatggtgtct tttcccacat gtagtgagag agcgtatttt
     6361 tccacattgt cgcgctcggt cccgagaaga gcgatgcagc gaggcatgag tgtcgtcgca
     6421 ttggcttgcg ccgtgttcaa atcgcggcga agtgcctcaa gatcactccg gctcgccgcg
     6481 cctaaattga tattttttga aatcgccggc ggctcaactt cagttgacaa cttttcagtt
     6541 gcagcgttgg tttcattcgc agccttggtt accattgcta tcatctgcaa gaatttattc
     6601 gagggatgtt gcttgagcgc ctcatctatt tgcctgggat cggtgatgcc ttgcaacgcc
     6661 gtctgacttt cacgagcggc aatactctcc tgcagtttgg ccaagtcggc tacgaaatat
     6721 gccccaaccg aaaagacaac cataaaaacg aataacaata agaaggcgat tcggacgcgc
     6781 gtgttcgctt tcggatctga aggcatttcc atcggcctca catcgcgata cgcccctgta
     6841 gggggaaggg acgacgagaa taatagattt ggttctgtca aacgtcgact gggcgaatca
     6901 ccagactccg ccgttccaac agatcacaca gtcttaatca tagcctcgca gggcgggctt
     6961 tcccaacggg tccgcgccaa cgggtcgcgc gaatgcgcgc ctgatgacag gcaaggcacg
     7021 gcccgatggg cgtaacccgc ccctctatcg cccgtgaacg acggattaag cggagtttat
     7081 cgtcgggcgc gcattcgcgc gacgcgctga ctaatccgcc ctcccgggct cgaattactt
     7141 cgccgaggcc gattgctcgg ttggtttgcc gaaaacgcgg cgacaaccct cgcttagact
     7201 ggaccgttgc ttgtgcatgc aggccgtaat acgctcgaca ttcggaattt ccga
//
LOCUS       DASWGM010000954         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1621591,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000954 DASWGM010000000
VERSION     DASWGM010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621591"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGA15_25700"
     CDS             complement(<1..513)
                     /locus_tag="VGA15_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591122.1"
                     /translation="MSDAQTEAMVLDVKGLRTVFFTNSGLFKAVDDVSFSVRRGETLA
                     IVGESGCGKSVTALSVMRLVPDPPGRIVGGSIVLEGTDLLGLDESEMRAIRGNRISMI
                     FQEPMTSLNPVMRIGDQITEAVRLHRPMSRKEATAKAVEMLRLVKIPEAERRAQEYPH
                     QLSGGMRQRAM"
     gene            complement(513..1406)
                     /locus_tag="VGA15_25705"
     CDS             complement(513..1406)
                     /locus_tag="VGA15_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9591123.1"
                     /translation="MDAINYDTELRRAGASSTHGWRKLTFLAQRHMLGTIGLVIMVMF
                     VLAAALADGICRFSPLTVDSAHALSAPNVQHWMGTDSFGRDVWSRIIHGARISLAVGI
                     GSTALGSSIGVMIGLASGYLSGWVDLVCQRVIDILQALPLLVLALVMSAALGPSLPNT
                     IIAIAIPLIPTVARVIRANTLALRELAFVEAAKSIGMSEMRIALRHVLPNTLAPLIVL
                     ATAQLGAAILTEASLSFLGLGIPEPYPSWGRMLSESAAEYVRTAPWLVIFPGVAISLA
                     VFGTNLLGDALRDILDPRQRG"
     gene            complement(1415..2362)
                     /locus_tag="VGA15_25710"
     CDS             complement(1415..2362)
                     /locus_tag="VGA15_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9591124.1"
                     /translation="MFAYIVRRLVLMLVTLFGISVIIFVLLRIVPGNIVDILFAAAGY
                     VDPADKANLERELGLDQSIVMQYLHWIGGLLRGDLGYSYVSEKPALDEILPRIPITAR
                     LAGLALLFSASIGIPLGVISAVNQGTRLDYILRIVSLSGLSLPSFWLGLLILMASVAL
                     FGTMPIFNPNPQGFGEACAIYCVPALAVGFRSAALVMRITRSSMLEILRQDYIRTARA
                     KGASERAVNYHHALKNAILPVITVIGIEAAFLVGGLIVTETVFNIPGVARFLVEAIRW
                     RDYPIVQNLVMLIALVVVVANFTVDMLYAAFDPRIRYTD"
     gene            complement(2493..>2878)
                     /locus_tag="VGA15_25715"
     CDS             complement(2493..>2878)
                     /locus_tag="VGA15_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9591125.1"
                     /translation="DEVETANWFSKVARKDYALGLNLTGNAVDDPDQAFYENYSCNSE
                     RNYTNYCNKDIEKLFDQQSQETDVAKRKKLVWEIDKKLQEDVARPIIFHGRLGSCWQP
                     YVKGITIMVNSSYNGYRYEDVWLDR"
BASE COUNT          592 a          874 c          897 g          515 t
ORIGIN      
        1 catcgcgcgc tggcgcattc cgcccgagag ctgatgggga tattcctggg cgcgccgctc
       61 cgcttccggg attttgacga ggcgcagcat ctcgacggcc ttggccgttg cctctttccg
      121 gctcatcggc cgatgcaggc gaaccgcctc ggtgatctgg tccccgatcc gcatcaccgg
      181 gttgagtgag gtcatcggct cctggaagat catcgagatg cggttgcccc ggatcgcgcg
      241 catctcgctc tcatcgaggc cgagaagatc ggtgccttcg agcacaatgg agccgccgac
      301 aatccggccc ggcggatcgg gcaccaggcg catgaccgaa agcgccgtca cgctcttgcc
      361 gcagccggac tcgccgacga tcgccaaggt ttcgccgcga cggacgctga acgaaacgtc
      421 gtcgacagcc ttgaacagcc cggaattcgt gaagaaaacc gtccgcaggc ccttcacgtc
      481 gagcaccatc gcttccgttt gcgcatcgct catcagccgc gctgcctcgg gtcgaggatg
      541 tcgcggagcg cgtcgccgag caggttggtt ccgaacacgg cgagactgat cgccaccccg
      601 gggaaaatca ccagccaggg ggcggtgcga acatattcgg cggccgattc cgaaagcatg
      661 cgcccccatg acgggtacgg ctcgggaatc ccaagaccga gaaacgacag cgatgcctcc
      721 gtcagaatgg cggcccccaa ctgggcggtg gcaagcacga tcaacggcgc cagcgtgttc
      781 ggcaacacgt gccgaagcgc aattcgcatc tcgctcatgc cgatcgactt ggcggcctcc
      841 acgaacgcaa gctcgcgcag cgccagggtg ttggccctga taacgcgggc gactgtggga
      901 atgagcggta tcgcgatcgc gatgatggtg ttcggcagcg acggccccag cgcggccgac
      961 atcaccagcg cgaggaccag cagcggcagc gcctgcagaa tatcgatcac ccgctggcaa
     1021 accagatcga cccagccgga cagataaccc gaagcgagac cgatcatcac gccgatggac
     1081 gagccgagcg cggtcgaccc gatgccgacg gccagcgata tgcgcgcgcc gtggatgatc
     1141 cggctccaga catcccggcc gaacgagtcc gtgcccatcc agtgttgtac gttgggcgcc
     1201 gaaagcgcgt gcgcggaatc aacggtcaac gggctgaagc gacagatgcc gtcggccagc
     1261 gccgcggcca gcacgaacat caccatgatg accaggccga tggtgccgag catatggcgc
     1321 tgcgccagga acgtgagctt gcgccagccg tgcgtcgaac tggcaccggc gcgtctcagt
     1381 tcggtatcgt agttgatcgc gtccaattga cctcctaatc cgtgtatcgg attcgcggat
     1441 cgaacgccgc gtagagcatg tcgaccgtga agtttgcgac cacgacgacc agggcaatca
     1501 gcatcaccag gttctggacg atcggataat cgcgccagcg gatcgcttcc accagaaagc
     1561 gggcgacgcc tgggatattg aacacggttt cggtgacgat caatccgccg accagaaaag
     1621 ccgcttcgat gccgatcacg gtgatgacag gaagaatggc gtttttgagc gcatggtggt
     1681 agttgacggc ccgttcggaa gcgcccttgg cgcgcgccgt gcggatgtaa tcctgccgca
     1741 atatttcaag catcgatgaa cgcgtgatcc gcatcaccag tgcggcgctg cggaagccga
     1801 ccgccaatgc cgggacgcag tagatcgcgc atgcctcgcc aaagccttgt ggattggggt
     1861 tgaagatcgg catggttccg aacagggcga ccgaggccat caggatcagc aggccgagcc
     1921 agaacgatgg cagcgacagg ccgctcaggc tgacgatgcg caggatataa tccagccggg
     1981 tgccctgatt gaccgcgctg atgacgccca gcggaatccc gatcgacgcg gagaacagca
     2041 acgccaggcc ggccagccgc gccgtgatcg gtattcgcgg caggatttcg tcgagcgcgg
     2101 gcttttcgga tacataggaa tagccgagat cgccgcgcag cagtccgccg atccagtgca
     2161 gatactgcat gacgatcgat tgatcgagcc cgagttcgcg ttccagattg gccttgtcgg
     2221 ccgggtcgac atatccggcg gcggcaaaca ggatgtcgac gatattgccg ggtacgatcc
     2281 gcagcagcac aaagatgatg accgagatcc cgaacagggt cacgagcatc aatacgaggc
     2341 gccgcacgat ataggcaaac aactggctgc tcccgttggc gcgtattttc gcaaaagtgg
     2401 ataccggttt tgcgatcgga atacgcgcaa gttattaagc cgactggcga ggctttatac
     2461 cggcaaggtt ttacattcgc tattggtcga cgttatctat ccagccacac gtcctcgtag
     2521 cgatagccgt tgtaggagct gttcaccatg atggtgatgc ccttgacgta gggctgccag
     2581 cacgagccca ggcgaccatg gaagatgatc ggacgggcga cgtcttcctg aagcttcttg
     2641 tcgatttccc agaccagctt cttgcgcttg gcaacatcgg tctcctgaga ttgctggtcg
     2701 aacagcttct cgatgtcctt gttgcagtaa ttggtgtagt tgcgctccga attgcaggag
     2761 tagttctcgt agaacgcctg gtccggatcg tcgaccgcgt ttccggtgag attgaggccg
     2821 agcgcatagt ccttgcgcgc cactttcgag aaccaattgg cggtttcgac ctcgtcca
//
LOCUS       DASWGM010000955         5690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1215757,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000955 DASWGM010000000
VERSION     DASWGM010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215757"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="VGA15_25720"
     CDS             <1..1634
                     /locus_tag="VGA15_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9591126.1"
                     /translation="AVEKFYTTRDFAPLWTQAGGLNDSGKGVVARLRDAASDGLNAAD
                     YQVPDFGAATTPDALAEADLKLTASMLDYARQAQSGRMHWSQVSGDIQYPEHPIDPAE
                     VLTNVTTAKDASAALDGYNPPQKLYRELKAKLAELRGQGEGKVVPLWEGPALKFTPAR
                     KKQPAAELMEDARVPLLRAKLGITESADDAHYDAKVAEAVRKFQEGADLKATGILDDR
                     TVKALNSPKRDRQIDTVIVNMERWRWLPRDLGAPAIGDAYVILNIPDYTLKVMQHGAQ
                     VWTTKVVTGQPGIHATPLLTETMKFITVNPTWNVPPSIIYNEYLPALQQDPTVLDRMG
                     LKLEHARDGSIHISQPPGEANALGRIRFNFPNKFLVYQHDTPDKNLFNREERPFSHGC
                     MRVQNPDQYAATLLNITAPNEHYTPEKIRGMYGHSEIDIKFPTPIPVNITYQTAFVDD
                     SGRLQLRKDVYGRDGTMLALLRNSRSKDLENVVAHSQPNYVRPPGNLPTGVSFASDNT
                     FSSGPNFFERLFGGPTAPPPVPSRRVPQQRRLFAR"
     gene            1904..3502
                     /locus_tag="VGA15_25725"
     CDS             1904..3502
                     /locus_tag="VGA15_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX9591127.1"
                     /translation="MLAGFARQFNVLSLSKAGYHLGLTSLLLLAGAGSVHDAAALNET
                     RTLSFHHTHSDEDLTVTFKRDGRYDEEALKQLNHYLRDWRSQEQTTMDRHLFDILWEV
                     YRDVDGKKPIQIVSSYRSPSTNAMLRRRSSGVARFSQHMLGHAMDFFVPDVPLEQIRF
                     AGLRLQRGGVGFYPTSGSPFVHLDTGSIRHWPRMTHDQLANVFPDGRTVHVPSDGNPL
                     KGYDLALADIEKRGDGDGVSTKSKPGLFAALFRGKSNDEEDEGASAPVAGEATAPAGP
                     MAASPAKSADAVPIPRSKPQAGSTLQLASADAQIVQPAKSRQAAAASAEKAEPKPQTP
                     ADIINARGFWGDAPTKPKQATPAQVAAISARQAVTAADPQATASSAAFEALAYAPPSN
                     SPVDRSNIVAASAPIPRSFRPASAPRNPMAATTIDQVVAKGPQGQGSAIATSTRIAAA
                     TGNGVWMRVMMLAPSASTSMSTTVLGDTDMTLMRIHFVKPQAIVAMSFSDDPQMGMVS
                     DRFTGPAIATLATQSFVMRTASLH"
     gene            complement(3655..3927)
                     /locus_tag="VGA15_25730"
     CDS             complement(3655..3927)
                     /locus_tag="VGA15_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEX9591128.1"
                     /translation="MATSAAAVKEEPATTFAKDQLKAIVERIERLEEEKKTISDDIRD
                     VYAEAKGNGFDVKALRTIVRLRKQDANERAEEETILETYMQALGML"
     gene            complement(4231..4533)
                     /locus_tag="VGA15_25735"
     CDS             complement(4231..4533)
                     /locus_tag="VGA15_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEX9591129.1"
                     /translation="MAIDDKTRTELEAAVFRRLVEHLRSRTDVQNIDLMNLAGFCRNC
                     LSNWLKDEADAKGVDMSKDESREAVYGMPYDTWKSTYQGTASPEQLAAMKKAHSGH"
     gene            4741..5397
                     /locus_tag="VGA15_25740"
     CDS             4741..5397
                     /locus_tag="VGA15_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEX9591130.1"
                     /translation="MIAKDSHRPQRFPARMTAGLMPALALAMMSLWNNPAAADFRLCN
                     NTSSRVGIALGYKDAEGWTTEGWWNVSSRACETLLRGTLVARFYYIYALDYDRGGEWS
                     GQAFMCSRDKEFTIKGTENCLARGFDRTGFFEVDTGEQRAWTVQLTESNEQAGQRVPG
                     IPGTVDPGGAGGLPGMSNSPGGAPGLSTSPGGTPGLPNSPGGTPPGASGLPPAPGSKP
                     "
     gene            5394..>5690
                     /locus_tag="VGA15_25745"
     CDS             5394..>5690
                     /locus_tag="VGA15_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX9591131.1"
                     /translation="MRRLRRIKILATLGPASSDSAMIRRLFEAGADVFRINMSHTSHD
                     TMRELVKTIRNVESSYGRPIGILVDLQGPKLRLGSFAEGSIQLKNGESFVLDSDK"
BASE COUNT         1084 a         1867 c         1690 g         1049 t
ORIGIN      
        1 ccgcggtcga gaagttttac accacgcgtg attttgcgcc gctgtggacc caggccggcg
       61 gtctgaacga cagcggcaag ggcgtcgtcg cgcgtctcag ggatgctgcc tccgacggtt
      121 tgaacgccgc cgattatcag gtgccggatt tcggcgcggc gaccacgccg gacgcgctcg
      181 cggaagccga cttgaaactg accgccagca tgctggacta cgcgcgccag gcgcagagcg
      241 gccggatgca ctggtcgcag gtcagcggcg acatccaata cccggagcat ccgatcgatc
      301 ccgccgaagt gctgaccaac gtcacgaccg ccaaggatgc atccgcggcg cttgacggct
      361 acaacccgcc gcagaagctg tatcgcgaat tgaaggccaa gctcgccgag ttgcgcggcc
      421 agggcgaagg taaggtggtt ccgctttggg aagggccggc gctcaagttc acgccggctc
      481 gcaagaaaca gcccgctgcc gagctgatgg aagatgcgcg cgtgccgctg ctgcgcgcca
      541 agctcggcat caccgaaagc gccgacgacg ctcactacga tgccaaggtc gccgaggccg
      601 tgcgcaaatt ccaggaaggc gccgacctca aagccaccgg cattctggat gaccgtaccg
      661 tgaaggcctt gaacagcccc aagcgcgacc gccagatcga caccgtgatc gtcaacatgg
      721 agcgttggcg ctggctgccg cgcgatctcg gcgcgcccgc gatcggcgat gcctacgtca
      781 ttctcaatat tccggactat acgctcaagg tgatgcagca cggcgcgcag gtctggacca
      841 ccaaggtggt caccggacag cccggcattc atgccacgcc gctgctgacg gaaaccatga
      901 agttcatcac ggtcaatccg acctggaacg tgccgccatc gatcatctat aatgaatacc
      961 tgcccgcgct gcagcaggat ccgaccgtgc tggaccgcat ggggctgaag ctcgagcacg
     1021 cccgcgacgg cagcatccac atctcgcagc cgcccggcga agccaacgcg ctcgggcgca
     1081 ttcgcttcaa ttttccgaac aagttcctgg tctaccagca cgatacgccg gacaagaatc
     1141 tgttcaaccg ggaagaacgg ccgttcagtc atggctgcat gcgggtgcag aatccggatc
     1201 aatacgccgc gacgctgctg aacatcacgg caccgaacga gcactacacc cctgaaaaaa
     1261 tccgcggcat gtacggtcac agcgagatcg acatcaaatt cccgacgccg ataccggtga
     1321 acatcaccta ccagacggcg tttgtggatg attcgggcag gctgcagctg cgcaaggacg
     1381 tctacggccg cgacggcacc atgctggcgc tgctgcgcaa ctccagaagc aaggatctgg
     1441 agaacgtggt cgcgcattcc cagccgaatt acgtccggcc gcccggcaat ctgccgactg
     1501 gcgtgagctt cgccagcgac aacacttttt cttccggacc gaacttcttc gagcggctgt
     1561 tcggggggcc gacggctcct cccccggtgc cgagccgtcg cgtgccgcag caacggcggt
     1621 tgttcgcccg ctgattttcc accgctttct gcagctggtc ggtatttcca agcagaatca
     1681 atggcctcgc tattttggcg aggccatttt cattaaccat tctccgccct cggttagccg
     1741 gcggcacttt ttcgccacac tcgccgggtt aggttaacac tgactttggg ggatgggcgc
     1801 aattctcgac aaaccctccc acgttaaggc tgcgttaatc ctctttcgcc acacggggta
     1861 gcgagagagt tcatcgatcg gtctattctg ggtgggctca tacgtgctgg ctggtttcgc
     1921 acgccaattc aatgtgctgt cgttgtcgaa ggccgggtat catctcggcc tgacatcgct
     1981 attgcttctg gccggagccg gatcggtcca cgatgcggcc gcgctcaacg aaacccgcac
     2041 cctttcgttt caccacaccc attccgatga agatctcacc gtcaccttca agcgcgacgg
     2101 gcgctacgac gaagaggcgc tgaaacagct caatcattat ctccgcgact ggcgcagcca
     2161 ggagcagacc acgatggatc gtcatttgtt cgacatcctc tgggaagtct accgcgacgt
     2221 cgacggcaag aagccgatcc agatcgtctc gtcgtaccgc tccccctcta ccaacgccat
     2281 gctgcgccgc cgatcgtcgg gggtagcccg cttcagccag catatgctgg gccacgccat
     2341 ggatttcttc gttccagacg taccgctcga gcagatccgc ttcgccgggc ttcgcctgca
     2401 acgcggcggc gtcgggttct acccgacatc gggatcgcct ttcgtgcatc ttgataccgg
     2461 aagcattcgc cactggccac gaatgaccca cgatcagctg gccaacgtat tcccggacgg
     2521 ccgcaccgtg catgttccgt cggacggcaa tccgctcaag ggctatgacc ttgctctcgc
     2581 cgacatcgaa aaacgcggcg acggcgacgg cgtttcgacc aagagcaaac cgggcctgtt
     2641 cgccgctctg ttcaggggca agtcgaacga cgaggaggac gagggcgcca gcgctcccgt
     2701 cgccggcgaa gccaccgccc ccgctggccc aatggccgct tctcccgcca aatccgccga
     2761 cgccgtgccg ataccgcgct cgaaacctca ggccggttcg accctccagc tggcttcggc
     2821 cgacgcgcag atcgtccagc ccgcaaaatc caggcaggcc gccgcggcgt cagccgaaaa
     2881 ggccgaaccg aagccgcaga cgccggctga tatcatcaat gcccgcggct tctggggcga
     2941 cgcccccacc aagccgaagc aggcaacgcc cgcgcaggtc gccgccatca gcgcccgcca
     3001 ggcggtcacc gccgccgacc cgcaagcgac tgcaagttcg gccgcgttcg aggccttggc
     3061 ctacgcgcca ccatccaatt caccggtgga ccgctcgaat atcgtcgcgg cgagcgcgcc
     3121 gataccgcgc agcttccggc cggcctcggc tccccgcaat ccaatggccg ctaccacgat
     3181 cgaccaggtc gtcgccaagg gaccgcaggg ccaaggcagc gcgattgcga catcgacgcg
     3241 gattgccgcg gccaccggca acggtgtctg gatgcgcgtc atgatgctgg cgccgagcgc
     3301 gagcacgtcg atgtccacga cagtcctggg cgataccgac atgaccttga tgcggatcca
     3361 tttcgtcaag ccgcaggcca ttgtcgcgat gagcttctcg gacgatccgc agatggggat
     3421 ggtcagcgac cggtttaccg gaccggcgat tgcgacgctg gcgacgcagt cattcgtgat
     3481 gcggaccgca tcgctgcact gagggacatc gtcattgccg ggcatagccg tccgaaggac
     3541 ggcgtcgctt tcgttcgcct acgccccggc aatccatccc cttcgtatga acctttgtga
     3601 agattgatgg atacgcgggt caagcccgcg tatgacagca tggcctgttc ggcctcacag
     3661 cattcccagc gcctgcatgt aggtttcgag aatggtttcc tcctcggcgc gctcgttggc
     3721 gtcctgcttg cgcagacgca cgatcgtgcg cagcgccttg acgtcgaagc cgttgccctt
     3781 ggcctcggcg tagacgtcgc ggatatcgtc ggagatggtt ttcttctctt cttccagccg
     3841 ctcgatgcgc tcgacgatgg ccttgagctg atcttttgca aatgttgtcg cgggctcttc
     3901 cttgacggca gcggcagagg tggccatcgg atactcctga ttgaatgggt gattgagtca
     3961 aaagcggaag gcgccgacgc ttcgccacgc accgcatccc ggaatgaccc tcgtgagtgg
     4021 gacgacctgc gtcaaggcaa cagcgcgctc atccacagcg cgcccacagg agatagcgaa
     4081 atccctctcc gcaaaatgaa gaagcttgaa tttctggaac tacgcgccgc gtggtcgcca
     4141 tgccgcgaat cgcgcgccga cgtcgcggca gttcaagtcc cgcgccgccc acatgcgcgg
     4201 ggagccgtgc tgcgacaaga tcgaaacagg tcaatgcccg ctatgggcct tcttcatcgc
     4261 cgcgagttgc tcgggactgg ccgtcccctg ataagtcgac ttccaggtat cgtagggcat
     4321 gccgtagacg gcctcgcggc tctcgtcctt gctcatgtca acgcctttgg cgtcggcctc
     4381 gtccttgagc cagttggaca ggcagttgcg gcagaagcct gcgagattca tcagatcgat
     4441 gttctggaca tcggtgcggg aacgcagatg ttcgaccagc cgccggaaca ccgcggcttc
     4501 aagttccgtt ctggttttgt cgtcaattgc catgaccgta tctcgaccgt tatctcgcgg
     4561 ttgcgcgtaa gcttattgat cttaggtggg gagttgtcac agattttacc atatcgaagc
     4621 gcaaagacag ggttaacaag cgggatttcg gctgccgaat gcctgaaaat gtcccctcgg
     4681 ccgttgacag ctgccgctgg cttacgcgaa atcatcaatg atttgatata gcttcactga
     4741 atgatcgcaa aagattccca tcgcccccag cgtttccctg ctcgcatgac cgccggtttg
     4801 atgccggcgc tggcgcttgc aatgatgtcc ctgtggaaca atccggcggc ggcggacttc
     4861 cgtctctgca ataacacctc gagccgcgtc ggcattgcgc tgggctacaa ggatgccgag
     4921 ggctggacca ctgagggctg gtggaacgtt tcctcccggg cctgcgagac cctgctgcgc
     4981 ggcacgctgg tcgcacggtt ctactatatc tatgcgctcg actacgaccg cggcggcgag
     5041 tggtcaggac aggccttcat gtgctcccgc gacaaggaat tcaccatcaa gggcaccgaa
     5101 aactgcctgg cgcgcggttt cgaccgcact ggctttttcg aagtcgatac cggcgagcag
     5161 cgtgcctgga ccgtgcaact gaccgaatcc aacgaacagg ccggacagcg ggtgccggga
     5221 atcccgggaa cggttgatcc cggcggagcc ggaggccttc cgggcatgtc gaattcgccg
     5281 ggcggcgcgc ccggcctgtc cacctcgccg ggcgggacac ccggcctgcc caattcgccg
     5341 ggcgggacgc ctccgggcgc gtctggtctg ccgccagcgc ccggaagcaa gccatgagac
     5401 gcctgcgccg catcaagatc ctggcaacgc tcggtccggc atcctccgac agcgcgatga
     5461 tccgccgcct gttcgaggcc ggcgccgatg tcttccgcat caatatgagc catacctcgc
     5521 acgatacgat gcgcgaactg gtcaagacca ttcgcaatgt cgaatcgagc tacggccggc
     5581 cgatcggcat tctggtcgat cttcaggggc cgaagctgcg cctgggctcg ttcgccgaag
     5641 gctcgatcca gctcaaaaac ggcgaaagtt ttgtgctcga ttccgacaag
//
LOCUS       DASWGM010000956         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1215970,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000956 DASWGM010000000
VERSION     DASWGM010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215970"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VGA15_25750"
     CDS             <1..598
                     /locus_tag="VGA15_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9591132.1"
                     /translation="ESFALDVTQRENCVAVAKEIADKVGQVSVLVNNAGIARRNGMLG
                     ATEAVIKDWEDIIAINLTGVFNVTHAFLEPLRAKKGRIINIGSIQSFVHLRTPSSPAY
                     TASKHGVLGFTKALAAELGKDGVRVNAIGPGFIETPLNEKVRASNPELARTFIDHTPL
                     GRAGKPEDIVGPAIFLASDLSAYVTGSIVMADGGYRTV"
     gene            808..1980
                     /locus_tag="VGA15_25755"
     CDS             808..1980
                     /locus_tag="VGA15_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9591133.1"
                     /translation="MSNAPHFDIDVAAFWTDPYPDLARMRREAPIAFVPQLGSTIFTR
                     RDDIFSQEKRIDVFSSHQPAGLMNTLMGHNMMRKDGDAHMSERAAMFPAVSPRTVRDT
                     WLRQFQAHADHILDELAPRGRADLCKAFALPLSAECLKDITGLTNMRFEDMDAWSQAM
                     IDGIANYSGNKEVEARCHAATSGIDAAIDDMIPVVTKHPNTSILSMLLAAGQPMPSVR
                     ANIKLAISGGQNEPRDAISGTIWALLTHPDQLALVREGRARWLDVFEEYARWIAPIGM
                     SPRRVARHWSFGGVDFEPEDRVFFMFGSANRDEACFADPDRFDITRDTAKSIAFGAGP
                     HYCAGAFASRAMVADVALPGIFARLKGLRLDTAEPVRIGGWAFRGLLNLPVIWDVL"
     gene            complement(2060..2287)
                     /locus_tag="VGA15_25760"
     CDS             complement(2060..2287)
                     /locus_tag="VGA15_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591134.1"
                     /translation="MNPETQLKLCAIMFTVLWTGWMLWSSGSLDRVNVILMTICGAAA
                     GYAWYRVMRWQFRRSGLHSQDERSAGPAAKR"
     gene            complement(2382..2648)
                     /locus_tag="VGA15_25765"
     CDS             complement(2382..2648)
                     /locus_tag="VGA15_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX9591135.1"
                     /translation="MCRNIKTLFNFEPPATEDEIQASALQFVRKLSGFNKPSQANAEA
                     FDRAVAEVSASARRLLTSLHTHAPARDREIEAERAKERSRLRFG"
     gene            2793..3038
                     /locus_tag="VGA15_25770"
     CDS             2793..3038
                     /locus_tag="VGA15_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX9591136.1"
                     /translation="MSHVTYKIVQHDGGWAYTVDGVFSEPFATHAAALAAARRAAAEQ
                     RVPGRTEAIEYETADGKWHTETAAGSDRPETDVEDKV"
     gene            3182..>3559
                     /locus_tag="VGA15_25775"
     CDS             3182..>3559
                     /locus_tag="VGA15_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591137.1"
                     /translation="MKCSRAALSAVFAMTSFTTLAADYPTPKQADWVAREFKFHGGET
                     MPELRLHYTTLGEPGGQPVLVLHGSGGSAANLLTAAFAGELFGPGQPLDAAKYYIIIP
                     DSLGHGKSSKPSDGMKTAFPEYNY"
BASE COUNT          688 a         1154 c         1067 g          650 t
ORIGIN      
        1 ggagagtttt gccctcgacg tgacccaacg cgaaaactgc gtcgccgtcg ccaaagagat
       61 cgccgacaag gtcggacaag tctcggtcct ggtcaacaat gccggcatcg cgcggcgcaa
      121 cggcatgctg ggtgcgaccg aggccgtgat caaggattgg gaagacatca tcgcgatcaa
      181 cctcaccggc gtcttcaatg tgacgcatgc gtttctggaa cccttgcgcg ctaaaaaagg
      241 gcgcatcatc aacatcggtt cgatccagtc gttcgtgcat ctgcgcacgc cgagctcgcc
      301 ggcctatacc gcctccaaac acggcgtact cgggtttaca aaagcgctcg ccgccgagct
      361 cggcaaggac ggcgtacgcg tcaatgcgat cggcccgggt tttatcgaaa cgccgctgaa
      421 cgaaaaagtg cgcgccagca atccggaact ggcgaggaca ttcatcgacc acacgccgct
      481 cggccgcgcc ggcaagccgg aggatatcgt gggacctgcg atttttctag cgtcggatct
      541 ttcggcctat gtcacaggat cgatcgtgat ggcggatggc gggtaccgga ccgtttgaat
      601 ctgaagtcga gaagcgcacc gcgtacgcaa cattcacagt cgtcatcgcc cggcttgacc
      661 aggcgaccca gtattccaga gtgcttatga tggaatcgaa aagccgcggc gtattggatg
      721 ccccgctttc gcggggcatg acgaccgcta tattggaggc gacgtgcgca aggatttcaa
      781 actgcaaaaa ctccgatagg aaacgccatg tccaacgccc cgcattttga catcgacgta
      841 gcggcgttct ggaccgatcc ctatcccgat ctcgcgagga tgcgccggga ggcaccgatc
      901 gcgttcgtgc cgcagctcgg ctcgaccatc ttcacgcggc gcgacgacat cttttcgcag
      961 gaaaagcgca tcgacgtgtt ctcgtcgcat cagccggccg gattgatgaa tacactgatg
     1021 ggccacaaca tgatgcgcaa ggacggcgac gcgcatatgt cggagcgcgc ggcgatgttt
     1081 cccgccgtgt cgccgcgcac cgttcgcgac acctggctca ggcagtttca ggcccatgcc
     1141 gaccacatcc tcgacgagct cgcgccgcgc ggccgcgccg atctctgcaa ggcgttcgcg
     1201 ctgccgctgt cggccgagtg cctgaaggac atcacgggtc tgaccaacat gcgctttgag
     1261 gacatggacg cgtggtcgca ggcgatgatc gacggcatcg ccaattacag cggcaacaag
     1321 gaggtcgagg cgcgctgtca cgccgccaca tcaggcatcg acgccgcgat cgacgacatg
     1381 attccggtgg tgacaaaaca tcccaacacc tcgatcctca gcatgctgct cgccgcgggc
     1441 cagccgatgc caagcgtgcg cgccaacata aaactcgcca tctccggcgg gcagaacgag
     1501 ccgcgcgatg ccatctccgg cacgatctgg gcactattga cacatccgga tcagctcgcg
     1561 ctggtgcgcg aaggacgcgc aaggtggcta gacgtgttcg aggaatatgc ccgctggatc
     1621 gcgccgatcg ggatgtcgcc gcgccgcgtc gcaagacatt ggtcctttgg cggcgtcgat
     1681 ttcgagccgg aggatcgcgt gttcttcatg ttcggctcgg ccaatcgcga cgaagcctgt
     1741 tttgccgatc ccgatcgttt cgacatcacg cgcgataccg ccaaaagcat cgccttcggc
     1801 gccggcccgc attattgcgc cggcgctttt gcttcacgcg cgatggtcgc cgacgtggca
     1861 ctgcccggca tttttgcgcg gctcaagggc ttgcggctcg acaccgcgga gccagtgcgg
     1921 atcggcggct gggcgtttcg cgggctgctc aatctgccgg tgatatggga cgtactttag
     1981 ggacgcgggt atcacggcga tgcatgtcgc ctcgcgaatc cagcagcgac cagcacgcct
     2041 tgattgcccg acatggcgtt taccgctttg ctgccggtcc ggccgaacgc tcatcctgcg
     2101 agtgcaggcc gctgcgccga aattgccagc gcatgacgcg gtaccaagca taccccgccg
     2161 ccgcgccgca gatcgtcatc aggatgacgt tgacgcggtc gagcgatccg ctcgaccaca
     2221 gcatccatcc cgtccacagg accgtgaaca tgatggcgca gagttttagt tgggtttcgg
     2281 ggttcaaggc ggctctccga gggcttgaaa aacttcaggc catcatgcgc gccacccccg
     2341 gaccccaccg tgaggtacgt cactgaaccg cgccaaaccc gtcatccaaa tctcagcctc
     2401 gaccgctcct tggccctctc ggcctcgatc tcgcggtcgc gtgccggcgc gtgggtgtgc
     2461 agcgatgtca gcaaccgccg cgccgacgcc gagacttccg cgacggcgcg gtcgaaggct
     2521 tcggcgttgg cctgcgacgg cttgttgaag cccgaaagtt tgcgcacgaa ttgcagcgcg
     2581 gaggcttgga tttcatcctc ggtcgcaggc ggctcgaaat tgaacagcgt cttgatgttg
     2641 cggcacatgg tttggctcct ttgccggtct cctcataccg aggacgattc acgcacgtaa
     2701 aaaacgacat gaccgcccat tttgtcatag aatgggcgac gcgttcaccc ccctgtcacc
     2761 gcgccccgca ccaacaaaga gcgagttccc caatgtccca cgtcacctac aaaatcgtcc
     2821 agcacgacgg cggctgggcc tataccgtcg atggcgtgtt ttccgaaccg ttcgcgaccc
     2881 atgccgcggc acttgccgcc gccaggcgcg ccgccgccga gcagcgcgtg cccggccgaa
     2941 ccgaggcgat cgaatacgaa accgccgacg gcaagtggca caccgaaacc gccgccggca
     3001 gcgaccgtcc ggagaccgat gtcgaggaca aggtctagat tattcccctg gtctcgcgcc
     3061 aattgttcgg aaattttttt tcggatcgtg gcgacgcccc catctcggat cgtgccgggt
     3121 ggcatgatag gcttgtcccc aagcggccca gctaacgcgg ccgcgccccg ggagtggcat
     3181 catgaaatgt tcgcgcgcgg cgctatcggc tgtctttgca atgacctcat ttacgacgct
     3241 ggcggccgat tatcccacgc caaagcaagc cgactgggtc gccagggagt tcaaattcca
     3301 cggcggcgaa accatgccgg agctgcgcct gcattacacc accttgggcg agccgggcgg
     3361 ccagccggtg ctggtgctgc acggttccgg cggctcggcg gcgaacctgc tgacggcagc
     3421 tttcgcgggc gaattgttcg gtcccggcca gccgctcgat gcggcgaaat attacatcat
     3481 cattcccgac agcctcggcc atggcaaatc gtcaaagccc tccgacggca tgaagaccgc
     3541 atttccggaa tacaactac
//
LOCUS       DASWGM010000957         3332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1621836,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000957 DASWGM010000000
VERSION     DASWGM010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621836"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VGA15_25780"
     CDS             <1..590
                     /locus_tag="VGA15_25780"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX9591138.1"
                     /translation="LLAATAQESMKHRCVVIGEDLGTVPKGFREQMADWGIWSYQVMM
                     FERDDHGSFRDIDYYAANALVTFNTHDLSTYAGWRSFSDLKLKRSLGIDPGESDDARW
                     HALTMLSEVLRHHAIDRHDLYAVVGFLARTKSRLLAISLEDLLGLIDQPNIPGTVNEH
                     PNWRRRLPLAIDGIAAAIDIPALRSATAERTRAAV"
     gene            620..2422
                     /locus_tag="VGA15_25785"
     CDS             620..2422
                     /locus_tag="VGA15_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX9591139.1"
                     /translation="MSCRIEDYGLIGDCETAALVGRDGSIDWLCWPAFDSDACFAALL
                     GTSKHGRWRIAPAEPVAKSWRRYWDNTLILETRFETASGVVALIDFMPPRGNASDVVR
                     LVRGVRGQVKLRMELVIRFGFGTDIPWVKRTEDGALLAICGQDMTVLRTPVETRGEDL
                     TTVADFEVGEGETIPFVLTYGPSHLPVPAPINPAQALQDTENFWTEWCSHCTHEGDNR
                     DLVIRSLITLKALTYGPTGGIVAAPTTSLPEKLGGARNWDYRFCWLRDATFTLLALMN
                     SGYTDEASAWHNWLLRAVAGAPANMQIMYGIMGQRRLLEWEADWLPGYEGAQPVRIGN
                     AAHAQLQLDVYGELIDAFHQSRVAELELDAGSWALECAVLEHLAEVWDQPDHGIWERR
                     GDGKHYVSSKVMTWVAFDRGIKSAEKFGFDAPLDRWRSLRDTICRDVCEKGFNATQNA
                     FVESYGSQLLDASILLLPAVGFLPASDPRVRGTIEAIEKYMMRDGFVLRHDPREISDE
                     RQPIEGAFLACSLWLADAHVLAGEIAKAQALFDRVVGTANDLGLLAEEFDSGEGRQTG
                     NFPQALTHIALINTAHNLSAAKEPLEKPAMQRSK"
     gene            2536..>3332
                     /locus_tag="VGA15_25790"
     CDS             2536..>3332
                     /locus_tag="VGA15_25790"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9591140.1"
                     /translation="MAATIVSAWLVLPAMAAGLDAATINNAEYRAKLPPDDRTDAAVV
                     KLQVLLDRARFSPGEIDGKLGENAQKALKAFADAKGLPSDKTLTPEIWKTLVGTSTDP
                     AITEYKISKSDVAGPFLEKLPVKMEQMKDLKALSYTSPREAIAEKFHMSEDLLAALNP
                     GKKFDQAGETVSVTNVLNKQTRLKVGRIEVDKSRQTVRAFAPSGELVAFFPATVGSEE
                     RPTPGGTLKVVSSDANPNYRYNPDYKFKGVKSKEAFTIKPGPNNPAGS"
BASE COUNT          666 a          986 c         1047 g          633 t
ORIGIN      
        1 cgctgctggc ggcgaccgca caggaaagca tgaagcatcg atgcgtggtg atcggcgaag
       61 atctcggcac cgtgccgaaa ggctttcgcg agcaaatggc cgattggggc atctggtcgt
      121 atcaggtgat gatgttcgag cgcgacgatc acggctcgtt tcgcgacatc gactattatg
      181 cggcgaacgc tcttgtcacc ttcaacaccc acgatctgtc gacttatgcg ggctggcgct
      241 ctttcagcga tcttaagctg aaacggtcgc tcgggatcga tcccggcgaa agcgacgacg
      301 cgcggtggca tgcattgacc atgctgagcg aggtgttgcg tcatcacgcc atcgaccgcc
      361 acgatcttta tgccgtggtg ggctttctgg cgcggaccaa atcccgcctg ctggcgatct
      421 cgctcgagga cctgttgggc ctgatcgacc agcccaacat tcccggcacc gtgaacgagc
      481 atccgaattg gcggcggcgg ctgccgcttg cgatcgacgg gatcgccgcg gcgattgaca
      541 ttcccgcgct ccgaagcgcg acggcggagc ggacccgagc cgcggtctga cgcacaacac
      601 tttcagcgag cgaaagaaat tgtcttgcag gattgaagat tatgggctga tcggcgattg
      661 cgagactgcg gcattggtcg gccgcgacgg atcgatcgac tggctatgct ggcccgcgtt
      721 cgattccgat gcctgttttg cggcactgct cggcaccagc aagcatggtc gatggcgcat
      781 cgcgccggcc gaaccggtcg caaaatcctg gcgccggtac tgggacaaca ccctgatcct
      841 ggaaacccgc ttcgagacgg caagcggcgt ggtcgcgttg atcgacttca tgccgccgcg
      901 gggcaatgct tccgacgtcg tccggctggt gcgtggcgtg cgcggccagg taaaactgcg
      961 gatggaactg gtgatccggt tcgggttcgg caccgatatc ccctgggtca agcgaaccga
     1021 ggatggcgcg ctgctggcga tttgcgggca ggacatgacg gtgctgcgaa cgccggtcga
     1081 gacccgcggc gaggacctga cgacggtggc cgattttgag gtgggcgaag gcgaaaccat
     1141 accctttgtg ctcacctacg ggccgtcgca tctaccggtc ccagcgccga tcaatccggc
     1201 gcaggccttg caggataccg agaatttctg gaccgaatgg tgcagccatt gcacccacga
     1261 aggcgacaac cgtgatctgg tgattcgatc gctgatcacg ctgaaggcac tgacctatgg
     1321 accgaccggc ggcattgtcg cagcacccac gacctcgctg ccggaaaagc tcggtggcgc
     1381 gagaaactgg gattaccggt tttgctggtt gcgcgacgcc acctttacac tgctggcgct
     1441 gatgaactcc ggctataccg acgaagcctc cgcctggcac aactggctgc tgcgtgcggt
     1501 tgcaggcgcg ccggccaata tgcagatcat gtacggcatc atggggcagc gacgcctgct
     1561 ggaatgggag gcggactggt tgcccggcta cgaaggcgcg cagccggttc ggatcggcaa
     1621 cgcggcgcat gcccagctgc agcttgatgt ctatggcgaa ctgatcgacg catttcacca
     1681 gtcgcgcgtg gccgagttgg agctcgatgc aggatcctgg gcgctggaat gcgcggtgct
     1741 ggagcatctg gcggaagtgt gggaccagcc ggatcacggc atctgggaac ggcgcggcga
     1801 cggcaagcat tatgtttcgt cgaaggtgat gacgtgggtg gcgttcgatc gcggtatcaa
     1861 aagcgcggaa aagttcggct ttgatgcgcc gctcgatcgc tggcgatcgt tgcgtgatac
     1921 catctgtcgc gatgtctgcg aaaagggttt caacgccacg caaaatgcgt tcgtcgaatc
     1981 ctatgggtcg cagttgctcg atgccagcat cctgctgctg cccgcggtcg gttttctccc
     2041 cgcctcggac ccgcgcgttc gcggcaccat cgaggccatc gagaaatata tgatgcgcga
     2101 cggattcgtg ctgcggcacg acccgcggga gatttcggac gaacggcaac cgatcgaagg
     2161 cgcatttctc gcctgcagcc tttggctcgc cgatgcccat gtactggccg gcgagatcgc
     2221 caaggcgcag gcgctgttcg atcgcgtggt cgggactgcc aacgatctcg gcctgttggc
     2281 ggaggaattt gattccggcg aagggcgtca gaccggcaat ttcccgcagg ccctcaccca
     2341 catcgcactg atcaatacag ctcataatct cagcgccgcc aaagaaccgc tggagaagcc
     2401 ggcgatgcag cggtcgaagt agcgacggac cggccgtgcg cagggggcgc cttcatttca
     2461 acgtggtgcg gaactcgcga gcctccaact tcttgtcgtc acgatattgc tggagggtgc
     2521 ctgatgatcc gcttcttggc cgcgacgatt gtatcggcct ggctggtgtt gcccgcgatg
     2581 gctgccggcc tggatgccgc caccatcaat aacgccgaat accgcgccaa gcttcccccc
     2641 gatgacagaa cggatgccgc cgtcgtcaag cttcaggttc tgttggaccg ggctcgattc
     2701 tcgccgggag aaatcgacgg caagctcggt gaaaacgcgc aaaaggctct gaaagccttc
     2761 gcggacgcca agggtttgcc gtctgacaaa accctcacgc cggagatctg gaagacgctc
     2821 gtgggaacga gcaccgaccc cgccatcacc gaatacaaga tctccaaaag cgatgtcgca
     2881 ggaccgtttt tggaaaagct gcccgtgaaa atggagcaga tgaaagatct caaggccctc
     2941 agctatacca gtcctcgcga agccatcgcc gagaagtttc atatgagtga agatctgctt
     3001 gcggctctaa accctgggaa gaaattcgac caggccggcg aaaccgtttc cgtcaccaac
     3061 gtgctcaata agcaaaccag gttgaaggtt ggccggatcg aagtcgataa gtcacgtcag
     3121 acggtcaggg ctttcgcgcc ctcgggcgag cttgttgcct tcttcccagc cacggttgga
     3181 agcgaggaga ggccgacccc gggcggcacg ctcaaggtcg tctcatccga cgccaatccg
     3241 aactatcgct ataatcctga ttacaaattc aaaggcgtca aatccaaaga ggctttcacc
     3301 atcaagccgg ggccgaataa cccggccgga tc
//
LOCUS       DASWGM010000958         4824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_798094,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000958 DASWGM010000000
VERSION     DASWGM010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798094"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(644..1567)
                     /locus_tag="VGA15_25795"
     CDS             complement(644..1567)
                     /locus_tag="VGA15_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9591141.1"
                     /translation="MARTRNGFTDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSR
                     QVSALEQELSVSLFHRHARGLILTEQGDLLFRTAHDVFMQLQAARAKLTDSRERPSGD
                     LKIETPPALGINWLIPRLDEFTALYPDIRISLIVTDEDLDLSMRAADVAIRTRKPTQP
                     DLIQRKLFSIGFHAYCSPEYIKRFGTPRTLDDLDTHRIIMLSDSQVPPHLANRSWLID
                     AGRNGSGPREAFFKVNNILGLVRACQQGLGIAALPDYLVEENNRLVQLFGESDSIQLD
                     TYFVYPEELKTVARVQVFRDFVVSKAQRWPS"
     gene            complement(1570..2535)
                     /gene="trxB"
                     /locus_tag="VGA15_25800"
     CDS             complement(1570..2535)
                     /gene="trxB"
                     /locus_tag="VGA15_25800"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX9591142.1"
                     /translation="MPAPIHAKVVIIGSGPAGYTAAIYAARAMLEPVLIQGIQPGGQL
                     TITTDVENYPGFADVIQGPWLMEQMEKQAAHVGTKIVTDLVTRLELAQRPFRLTCDSG
                     DIYLAETVILATGAQARWLGTASEEKFKGFGVSACATCDGFFYRGKQVIVVGGGNTAV
                     EEALFLTNFASQVTVVHRRDHFRAERILQERLFKNPKIKVVWDSAIDEICGTENPGKV
                     THVRLKNVRTGRVTELPADGVFIAIGHAPATELILGQIKLKPSGYVEVAPNSTATSVP
                     GVFAAGDVADETYRQAVTAAGLGCMASLEAERFLALRASDRAAAE"
     gene            2814..3293
                     /locus_tag="VGA15_25805"
     CDS             2814..3293
                     /locus_tag="VGA15_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9591143.1"
                     /translation="MSKNLDEIDLKILSEIQADGRITNVELAKRVGISPPPCLRRVRT
                     LEEAGYIQGYRGLLDPRRLGFDVTVFASVHLSSQAEADLRAFEEFVRAEPLVRECWML
                     SGEVDFILKCVARDMATFQDFVTNLTAAPHVRNVRTSLVLHNSKYEAAVPLDVKVLG"
     gene            complement(3407..3805)
                     /locus_tag="VGA15_25810"
     CDS             complement(3407..3805)
                     /locus_tag="VGA15_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9591144.1"
                     /translation="MNKILAAGEPVVLSIFRIITGLLLFQYGVAKLLKFPAGTMFEKV
                     QALSLFGVAGMFELVLGGLLILGLFSRLVAFILCGEMALAYFIEHFPRNFIPLLNNGN
                     LAIMLCFSCLYFACAGGGPWSLDAVMRGKR"
     gene            complement(3826..4221)
                     /locus_tag="VGA15_25815"
     CDS             complement(3826..4221)
                     /locus_tag="VGA15_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX9591145.1"
                     /translation="MIEALAAWRPRALSVLRIITGLMIIEHGMAKLIGFPAVATFANL
                     KPLSLLGAAGFIELIGGALLILGLLTRPAAFILSGEMAVAYFMVHAPKSFYPLINGGT
                     LAIMYCFACLYLSTAGAGPWSVDAAMKRD"
     gene            complement(4489..>4824)
                     /gene="greA"
                     /locus_tag="VGA15_25820"
     CDS             complement(4489..>4824)
                     /gene="greA"
                     /locus_tag="VGA15_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX9591146.1"
                     /translation="EYHAAKEEQSHNEGRIAELEDKLARADIIDISKLSGDTIKFGAT
                     VTLVDEDTDKKAVWQIVGEPEADAKKGRISITSPLARALIGKKKGASVEVVAPGGAKA
                     YEITKVEWR"
BASE COUNT          979 a         1409 c         1530 g          906 t
ORIGIN      
        1 gcggcgagaa tcaatccatt gacgggaaga tcggcgtaat gccgcgccag ccggcgcagt
       61 gacgcctcgt cgagttcgcc attgcgaaac ggcgtgacga gcggcagcca gaggccatgc
      121 aagcaaagtc gaagatcgga agttcgagag taggcggatc gaagctcggt catggtccat
      181 ctccttgggg ttgagccgga gacgggacaa taaaaaaccc cgtccagacg gcggggtttt
      241 gcgattcgtg ctgggcgaga agtctatcgc gcacgtcgat ctgcagttcc cggaagggga
      301 gcttttttcg accgcgctgc aatgcacata ttcggaatca tccgggaatg atgatgggcg
      361 aagcccggtc tgtcaacggt cgggaagacg acgtaacgtg tggcgtacgc gatcctcaaa
      421 aaaaagccgg gctcgaagag cccggcttaa ggtattggcc acgtgaggcc gacacaatca
      481 ccttccaaga gggattactg agcttccgcg ccactggagg agggggacat atgcgcaacg
      541 cgaatgctca gcgtggaagt agtatgatcc gcgtttacgc cttgcagcaa ccgtccagtc
      601 cgcatgtcag tcatgcggag atcgggggtt attgagacca ggatcaggac ggccagcgtt
      661 gcgccttgct caccacgaag tcgcggaaca cctgcacccg cgccaccgtc ttcaactctt
      721 cgggataaac gaaataggtg tcgagctgga tcgagtccga ttcgccgaac agctgcacca
      781 gacggttatt ttcctcgacc agatagtccg gcagtgccgc aatgcccaat ccctgctggc
      841 aggcgcggac cagccccaag atgttgttga ccttgaaaaa ggcctcgcgc ggccccgagc
      901 cattgcggcc ggcatcgatc agccagctgc ggttcgccag gtgcggtgga acctggctat
      961 cgctcagcat gatgatgcga tgcgtgtcga ggtcgtcgag ggtgcgcggc gtgccgaagc
     1021 gcttgatgta ttccggcgag caataggcat gaaagccgat cgagaacagc ttgcgctgga
     1081 tcagatcggg ctgggtcggc ttgcgggtgc ggatcgcgac atcggccgcg cgcatcgaca
     1141 gatcgagatc ttcatcggtg acgatcagcg agatacgaat atccggataa agtgcggtga
     1201 attcgtcgag ccgcgggatc agccagttga tgccgagcgc cggcggcgtc tcgatcttga
     1261 gatcgccgct cggccgctcg cggctgtcgg tgagcttggc gcgggccgcc tgcagctgca
     1321 tgaagacgtc atgcgcggtg cgaaacaaca ggtcgccctg ctcggtgagg atgaggccgc
     1381 gcgcgtggcg atgaaacagc gataccgaga gttcctgctc cagcgcgctg acctgccgtg
     1441 acaccgccga ttgcgacaga ccaagctgct cgccggcatg cgtgaagctg cctgcttccg
     1501 ccgccgcgtg aaacaccttc agtttgtccc agtccatatc ggtaaatccg ttgcgagttc
     1561 gagccatgac tattccgccg ccgcgcggtc gctcgcgcgc aaagcgagaa agcgttcggc
     1621 ttcgagggag gccatgcagc caaggcccgc ggccgtgact gcctgccgat aggtttcgtc
     1681 ggcgacgtcg ccggcggcga atacgccggg caccgaggtc gcggtcgaat tcggcgccac
     1741 ctcgacatag ccggagggtt tcagcttgat ctggccgagg atcagttcgg tggcgggcgc
     1801 atgcccgatt gcgatgaaga cgccgtcggc cggcagctcg gtgacgcggc cggtcctgac
     1861 gttcttcaag cggacatggg tgaccttgcc gggattttcg gtgccgcaga tctcgtcgat
     1921 ggcgctgtcc cagaccacct tgatcttggg gttcttgaac aggcgctcct gcaggatgcg
     1981 ctcggcgcgg aagtggtcgc gccgatggac gacggtcacc tgagacgcaa aattggtcag
     2041 aaacagggct tcctcgaccg cggtgttgcc gccgccgacc acgatgactt gcttgccgcg
     2101 atagaaaaat ccgtcgcagg tggcgcatgc cgatacgcca aaacccttga atttttcttc
     2161 cgatgccgtg ccgagccagc gcgcctgggc gccggttgcg agaatgaccg tttcggcgag
     2221 atagatatcg ccgctgtcgc aggtcagacg gaacggccgc tgggccagct caagcctggt
     2281 gacgagatcg gtgacgatct tggtgccgac atgggcggcc tgtttctcca tctgctccat
     2341 cagccaaggc ccctggatca cgtcggcgaa acccggatag ttttccacgt cggtggtgat
     2401 ggtgagctgg ccgccgggct ggatgccctg aatcaaaacc ggttcgagca tcgcgcgcgc
     2461 cgcgtagatc gctgcggtat agccggccgg gccggacccg atgatgacga ccttggcgtg
     2521 gatgggggca ggcatagagt cgatccctct cttaagggaa tttggcatgg attgatcagc
     2581 gcttaagcgg aatacgcccg ggaaggcatc gcgacggcgc tgaaagtgtc aaatccaaga
     2641 tagggtatgt ggcaagctat gcaagaattg caatccatat cggcaaattt tcccctccat
     2701 tacggtaaat ggcgcagcgc acgcgcaata aaattgcgca agatccacca gctgagctaa
     2761 gactgttgcc ggtattaccg ccagcattgg ccagccaagg aacccggacc ccagtgtcga
     2821 agaatcttga cgagatcgac cttaaaatcc tcagtgaaat ccaggccgac gggcggatca
     2881 ccaacgttga actggccaaa cgcgtcggca tttctcctcc cccctgcctg cgccgggtgc
     2941 gaacgctgga ggaggccggc tatatccagg ggtatcgcgg gctgctggat ccgcgccggc
     3001 tcggttttga cgtcacggtg ttcgcttcgg tgcatctgtc cagtcaggcc gaggccgacc
     3061 tgcgcgcgtt cgaggaattc gtgcgcgccg agccgctggt ccgggagtgc tggatgctgt
     3121 cgggagaggt cgacttcatc ctgaaatgcg tggcgcgcga tatggcgacg tttcaggact
     3181 tcgtcaccaa tctgacggcg gcaccgcatg tgcgcaacgt cagaacatcg ctggtgctgc
     3241 acaattcgaa atacgaggcg gccgtgccgc tcgatgtgaa ggttttgggc tgacgctgtc
     3301 gtcattccgg ggcgcgcggc agcgcgaacc cggaatctcg agattccggg tctgcgcttc
     3361 gcgcatcccg gaatgacggt cttttatttt agaaagccgc tcactctcag cgcttgccgc
     3421 gcatcaccgc atcgaggctc cacggtccgc cgccggcgca ggcgaaatag aggcaggaga
     3481 agcacagcat gatcgcgagg ttgccgttgt tcaacaacgg gatgaaattg cgcgggaaat
     3541 gctcgatgaa ataggcaagc gccatttcgc cgcacaggat gaatgccacc agccgcgaga
     3601 acaggccgag gatcaggagg ccgccgagaa ccagttcgaa catgccggcg acgccgaaaa
     3661 gcgaaagcgc ctggaccttc tcgaacatcg tccccgccgg gaatttcaac agcttggcca
     3721 cgccgtactg aaacagcagc aggccggtga tgatgcggaa gatgctgaga acgaccggct
     3781 cgccggccgc gagtatcttg ttcatcttgt ccatgttgcg ccccctcaat cccgtttcat
     3841 cgcagcatcg acgctccatg gacctgcgcc cgccgtcgac agatagaggc aggcgaagca
     3901 gtacatgatc gcaagcgtgc cgccgttgat cagcgggtag aagctttttg gcgcgtgaac
     3961 catgaaatag gcgaccgcca tttcacccga gagaatgaag gccgccggcc gcgtcagcag
     4021 gccgaggatc agaagcgccc cgccgatcag ctcaatgaag ccggcggcac cgagcagtga
     4081 tagcggcttc aggttggcaa aggtcgcaac cgccggaaag ccgatcagct tggccatgcc
     4141 gtgttcgatg atcatcaacc ccgtaatgat gcgcagcacg ctcaaggcgc gcggccgcca
     4201 cgctgcgagt gcttcaatca tgattattcc cccaatgttt ggcgcgccag accagtatcg
     4261 cgtcgcagcg ccgagggaaa ggatgtcgta tcaagaaaat cggcagcgtt ttgcaaagca
     4321 gcacaggaat aatgccgccg gcagagtgtt tgccgcgaag gtttcgacgg ccggtgttga
     4381 cgccagattc gtgacagcgc tgtcaaccaa gcggaaccaa caagggagcc gggaaaatat
     4441 cccggccctc ttcgttcgag acggaaattc gaaatgcttc gcctgggatc agcgccactc
     4501 gaccttggtg atctcgtagg ccttggcgcc gccgggcgcg acgacttcca cggaggcgcc
     4561 cttcttcttg ccgatcagcg cgcgcgccag cggcgaagtg atggagatgc ggcccttctt
     4621 ggcatcggct tccggttcgc caacgatctg ccacaccgct ttcttgtcgg tatcctcgtc
     4681 gaccagcgtg acggtggcgc cgaacttgat ggtgtcgccg gagagcttgc tgatgtcgat
     4741 aatatcggca cgcgcaagtt tgtcctccag ctcggcgatc cggccttcat tgtgggactg
     4801 ctcttccttg gcggcgtgat actc
//
LOCUS       DASWGM010000959         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1918467,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000959 DASWGM010000000
VERSION     DASWGM010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918467"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1900)
                     /locus_tag="VGA15_25825"
     CDS             complement(164..1900)
                     /locus_tag="VGA15_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9591147.1"
                     /translation="MASLSQQRLTRFIRGISVRQVRLASGLVLFGYLISHFLNHALGN
                     ISMDALAAGIYYHVAFWQFPPVGIVFYASALVHTGLGIWALYERRQFSWKAIEPLQLV
                     LGLSIPALIITHIIGVRLGQSLFGHEKLYPQVFFAYWIVWPPKMWLMYAVVIIAWIHG
                     CIGLNFWLRMKPFYQSAGPFLLGAAVLVPTLAVLGLYQGGRSIVDSDSVEWRAENLSP
                     DRVGTPAQQALLSSIEDYFLIGYLGLVGLVLLGKGARALHERRAGMITLSYGNGRTVR
                     MPRGLSVLEASLRNNVPHASVCGGRARCSTCRIRVIGDCTSLPEPSQREAFVLNRVGA
                     GSDPSIRLACQLRPETDLSFFQIFLPQITAATLRTSSPTRIGEERYLVSMFVDMRGST
                     RLAENRLPFDTVFIVNRFLGAVSQAVIECGGKPNQFVGDGQLALFGLNANPQTACRQA
                     LRAASMIAANVEQLNQFLEHDLREPIRFGIGIHGGEVIIGDIGYRDHMVFTALGDAVN
                     VAARLQDMTKSLACEAIVSEEVRVTAGLGADGLPEQEVAIRGRTEPMIVRTVTNASAL
                     SALVNELDVVAA"
     gene            2357..>3334
                     /locus_tag="VGA15_25830"
     CDS             2357..>3334
                     /locus_tag="VGA15_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9591148.1"
                     /translation="MTVLRVIYASVIDLRTDEAYYWTWSKESVLSFLDHPPMIAWFIR
                     FGTAIFGDTNLGVRFAGILAMLVTQLLLADIVRRVTHDVRAMVIAVLMPEAALYYGLL
                     MAKVSPDVALIPFAVAMVWALIRLNESNDPRWWLAAGVFAGLALLSKFTAVMLLPAVA
                     AFMLVPEWRKRWLTSLYPWASALIAVAVFSPALIWNAENDWASFKFQFVRVTATHEVS
                     LRTIGEYFGLQFGLVGFILLPVVLSGLALTAWRGYRRGNAVAILLSTCVIVPFGYFFW
                     KSLTLRVGDTWPMFMWPIGFAAAAINIAMLPREGFPAWMIKSTVAWANAA"
BASE COUNT          642 a          994 c         1055 g          643 t
ORIGIN      
        1 tgatgtgcgc cttggcgcgg gttggtcgtt tcagttctac ccatcgcaca tcagtcgttt
       61 cgctcaggag aggggttcga gaaaattgca tttcgttcga ggacaatttt ctgaacgaat
      121 attcagatgt gaaaaacatc acatactttc ggcgcgccgc cgctcaggcc gcgacgacat
      181 ccaattcgtt aaccaatgcg gacagcgcgc tcgcattggt gacggtgcgg acgatcatcg
      241 gctccgtgcg cccgcggatc gcgacttcct gctcgggcaa accatcggcc ccaagtccgg
      301 cggtgacgcg aacctcttcc gagacgatcg cttcgcaggc gaggctcttg gtcatgtcct
      361 gcagccgtgc tgcgacattg accgcgtcac cgagtgcggt gaacaccatg tgatcgcggt
      421 agccgatgtc gccgatgatg acttcgccgc catggatgcc gatgccgaag cggatcggct
      481 cgcgcagatc atgctcgagg aattgattga gctgctcgac attggcggca atcatgctag
      541 ctgcccggag cgcctggcgg cacgccgttt gcgggttggc gttgagcccg aacagtgcca
      601 actgcccatc gccgacgaac tggttgggtt taccgccgca ttcgatcacc gcctgcgaca
      661 ccgcgccgag gaaacggttg acgatgaaga cggtatcgaa cggcaaccgg ttttccgcga
      721 gcctggtcga gcctcgcatg tccacgaaca tgctgacgag atagcgctcc tcgccgatcc
      781 gggtgggact cgatgtccga agcgtggccg cggtgatctg cggcagaaaa atctggaaaa
      841 aggagagatc ggtctcgggc ctgagctggc aggcaaggcg gatcgaggga tcggagccgg
      901 ccccgacccg gttgagcacg aacgcctcgc gctgcgacgg ttccggcagc gacgtgcaat
      961 cgccgatcac gcgaatgcgg caggtggagc agcgggcgcg gccgccgcag acgctggcat
     1021 gcgggacgtt gttgcgcagg cttgcctcga gcacgctgag gcccctcggc atccgaaccg
     1081 tcctgccatt gccgtacgac agggtgatca tgccggcccg gcgctcgtgc agggcgcgcg
     1141 cgccttttcc cagcaagaca agtccaacca ggccgagata accgatcagg aaataatcct
     1201 cgatgcttga cagtaaggct tgttgggccg gggtgccgac ccggtccggc gaaagattct
     1261 ccgcccgcca ttcgacgcta tcgctgtcaa cgatgcttcg gccgccttga tagagaccaa
     1321 gcacggccag cgtcggaacc agcaccgccg cgccaagcaa aaacggcccc gcgctttggt
     1381 agaacggttt catccggagc cagaaattca gtccgatgca gccatgaatc caggcgatga
     1441 tcacgaccgc gtacatcagc cacattttgg gtggccaaac gatccagtag gcaaaaaaca
     1501 cctgcggata gagcttttcg tgtccgaaca ggctctggcc gagccgcacg ccgataatat
     1561 gggtgatgat gagcgcaggg atgctcaggc cgagcacgag ctgcagcggc tcgatcgcct
     1621 tccagctaaa ttgccggcgc tcgtaaagcg cccagatccc gaggccggtg tggaccaggg
     1681 ccgaggcata aaacacgatc ccgaccgggg gaaattgcca gaacgcgacg tggtaataga
     1741 taccggcggc aagggcatcc atcgaaatat tgccgagcgc gtggttgagg aaatggctga
     1801 tcaggtagcc aaacaacacc agcccggagg cgagacggac ctgccggacg ctgatgccgc
     1861 ggatgaatct tgtcagtcgt tgttgagaaa ggctggccat gcgatccaat cgaacagtta
     1921 tcgacaataa cgacgatagt agagttttat tccgccagcg accaatcttt ccggtcgtcc
     1981 cccgcgaacg ccgggacgtt ctttccctct ccccctgtgg gagagggtgg ccgcgatgcg
     2041 aagcatcgcg gacgggtgag gggtctcggc ctctcgatag aaagagaccc ctcatccggc
     2101 gcggacttcg catagccgat gcaaagcatc ggcgtcctac taaagaacgg cggccgaagg
     2161 ccgcctgcgg ccaccttctc ccacaagggg agaagggaag aaacacacaa tgctattccc
     2221 agcccagcac gttgacaccc catccggagt cggggagaaa gcgcccgtga cgattgccac
     2281 ccccgatagc agcagcacga aaccggacag tcttgtcctt cggcagctgc tcgccgttgc
     2341 cgcattgatc gtcgcgatga cggtgttgcg ggtgatctat gcaagcgtca tcgatctgcg
     2401 caccgacgag gcctattact ggacctggtc gaaggaaagc gtgctttcgt tcctcgatca
     2461 tccgccgatg attgcgtggt tcatccggtt cggcaccgcg atcttcggcg acacaaatct
     2521 tggcgtcagg tttgccggca tactggcgat gctggtcacg cagctgctgc tcgccgatat
     2581 cgtccggcgc gtcacgcacg atgtccgcgc gatggtgatt gcggtgctga tgccggaggc
     2641 cgccctttat tacgggttgc tgatggccaa ggtctcgccc gacgtggcgc tgatcccgtt
     2701 cgcggtcgcg atggtgtggg cgctgatccg gctcaacgag agcaacgatc cgcgctggtg
     2761 gctggccgcc ggcgtgttcg caggattggc gctgctgtcg aaattcaccg cggtcatgct
     2821 gcttccggcg gtggcggcat tcatgctggt gccggagtgg cgcaagcgct ggctgacaag
     2881 cctgtatccg tgggcgtcgg cgctgatcgc ggttgcggtg ttctcgccgg ctctgatctg
     2941 gaacgcagag aacgactggg cgtcgttcaa attccagttt gtacgggtaa cggcgaccca
     3001 cgaagtgtcg ttgcgcacga tcggggaata tttcggcctg cagttcgggc tggtcgggtt
     3061 catcctgctg ccggtggtct tgtcagggct cgcgctcacg gcgtggcggg gatatcgccg
     3121 gggtaacgcc gtggccattc tgctgtcgac ttgcgtcatc gtgcccttcg gatatttctt
     3181 ctggaaatcc ctcacgctgc gggtcggcga tacctggccg atgttcatgt ggccgattgg
     3241 atttgccgcc gccgccatca acatcgcgat gctgccgcgc gaaggctttc cggcatggat
     3301 gatcaaatcg accgtcgcct gggctaatgc tgcg
//
LOCUS       DASWGM010000960         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_524172,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000960 DASWGM010000000
VERSION     DASWGM010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524172"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VGA15_25835"
     CDS             complement(<1..141)
                     /locus_tag="VGA15_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9591149.1"
                     /translation="MKQLRIGDITIDAVIEREGPWRRPQDFFPAYDEAIFKQHLPSME
                     PEV"
     gene            complement(295..750)
                     /locus_tag="VGA15_25840"
     CDS             complement(295..750)
                     /locus_tag="VGA15_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9591150.1"
                     /translation="MTRSVSPKRSMKPSRPIYVLEEQVGFILRQVWQRHAAIFAREIG
                     INLTPTQWAALAKLTETGPCSQNQLGRLTAMDVATIKGVIDRLTARGLTETSPDPEDG
                     RRLLVSLTRAGQQMAEKAAPNALAITRETLAPLDAKERETLVVLLSKLR"
     gene            complement(783..1487)
                     /locus_tag="VGA15_25845"
     CDS             complement(783..1487)
                     /locus_tag="VGA15_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591151.1"
                     /translation="MKLSVQDLNSHYGPAHILFDIALEVDDGEVVALLGRNGAGKSTT
                     FRSIVGLVEQRTGRIVFEGKDVSDQPTHAIVRGGLGYVPEERRIFTDLTVDENLEVGR
                     QPIRPNAPHWTREKLFALFPNLGEMRGRPGGRMSGGEQQMLTIARTLMGNPSLVLLDE
                     PSEGLSPKIVEQMVDAILTMKKEGVSIVVSEQNLHFARLISDRAYIIERGRICFSGTM
                     SELDARPDVRDAHLAL"
     gene            complement(1484..2275)
                     /locus_tag="VGA15_25850"
     CDS             complement(1484..2275)
                     /locus_tag="VGA15_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591152.1"
                     /translation="MSIAPTLLAVEDLSKSYGGVHAVRKVSFALKAGEILALIGPNGA
                     GKSTCFDMLNGQNIPDSGRINLLGQDTAGKKPRAIWRLGVGRTFQITATFPTMTVREN
                     VQVALVSYRRQLFNLRASTPRSAREEAGRLLELVGMGGYAERPCGELAYGDLKRLELA
                     IALANQPKLLLMDEPTAGMAPRERIELMRLTAQIARQQSIGVLFTEHDMDVVFEHADR
                     ILVLNRGTLIAEGTPEEVRRNPQVRAIYLGEGLVYDISHREGAPS"
     gene            complement(2272..>3238)
                     /locus_tag="VGA15_25855"
     CDS             complement(2272..>3238)
                     /locus_tag="VGA15_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591153.1"
                     /translation="LAAMAALVLAAALPLIGGNYALTVGSEIAIFVIFAASLHFLMSV
                     GGLASFGHAAYFGLGAYGVALLGKLAGLPMILSLLLGPLLGCLGAAVFGFFAVQLSGV
                     YFAMLTLAFAQIVWSIAFQWVAVTGGDNGILGVWPEKWAAAPSHFYWLSIGVAALAVA
                     ALRIIVFSPFGYALRATRDSPLRSEAIGIDGKRIQWTAFVIAGTVAGIAGALFAYLKG
                     SVFPDSLGISLSVDALVMVLLGGVETVSGAVVGAIVYKALSIWLVSQTDLSKLVLGGV
                     IVLIVVAFPKGIVGMLETIRLRRRSEAPKPESSKPALAPGIETAE"
BASE COUNT          621 a         1057 c          992 g          568 t
ORIGIN      
        1 cacctccggc tccatcgacg gcagatgctg cttgaagatc gcctcgtcat aggccggaaa
       61 gaagtcctgc ggccgccgcc atgggccttc gcgttcaatc accgcgtcga tggtgatgtc
      121 gccgatcctg agctgcttca tcttcttgtc ttccccgaaa ttgccggggc accgtaatgg
      181 gcctccgtca accgttcaag ctgagggccg aaagattcaa cgagccggct atcgttgatg
      241 tcaacgtcag cgcgccgtcg tgttcgggca ggccctcgaa gcgatgacta caaatcatcg
      301 caacttgctg agcagtacga ccagcgtctc acgctctttc gcatcgagcg gcgccagcgt
      361 ctctctggtg atcgcgagtg cgttgggcgc ggccttttcg gccatctgct gaccggcgcg
      421 cgtgagactg accagcaggc gacggccatc ctcgggatca gggctggtct cggtcaggcc
      481 gcgggcggtg agccgatcga tcacgccctt gatggtggcg acatccatcg ccgtcagccg
      541 gccgagttga ttctgcgagc atggtccggt ctcggtgagt tttgcgagcg ccgcccattg
      601 cgtcggcgtc agattgatgc cgatctcgcg cgcgaagatc gccgcgtggc gctgccaaac
      661 ctggcgcaag atgaaaccga cctgctcctc aagaacgtaa atcggccgcg aaggtttcat
      721 gctccgtttt ggcgaaacac tcctggtcat atccttatcc ccgccctttt tcttgccggc
      781 gctcacaacg ccagatgcgc gtcgcgtacg tccgggcggg cgtcaagctc gctcatggtg
      841 ccgctgaagc agatgcggcc acgctcgatg atataggcgc ggtcggagat caatctcgcg
      901 aaatgcaaat tctgctcgga gacgacaatg ctgacgcctt ccttcttcat ggtcaggatg
      961 gcgtcgacca tctgctccac gatcttcggc gacagcccct ccgacggttc gtcgagcagc
     1021 accagcgacg ggtttcccat caaggtccgc gcgatggtca gcatctgctg ttcaccaccg
     1081 ctcatgcgcc cgcccgggcg gccgcgcatc tcgccaaggt tcggaaacag cgcaaataat
     1141 ttctcccggg tccagtgcgg cgcgttcggc ctgatcggct gacggcccac ttccaggttt
     1201 tcatcgaccg tcagatccgt gaagatgcgc cgctcttccg ggacatagcc gagcccgccg
     1261 cgcacgatcg catgggtcgg ctgatccgaa acgtccttgc cctcgaatac aatgcggccg
     1321 gtacgttgct cgaccagacc gacgatggag cggaaggtcg tggacttgcc ggcgccattg
     1381 cgtccgagca gcgcgactac ctcgccgtca tcgacctcaa gcgcgatgtc gaacaggatg
     1441 tgcgccggcc cgtaatgact gttgaggtcc tgaacggaga gcttcatgac ggcgctccct
     1501 cgcggtggct gatatcatag accaggcctt cgccgagata gatggcgcgg acctgcggat
     1561 tgcgtcggac ttcttcaggc gtgccttcgg caatcagtgt gccgcggttg agcaccagaa
     1621 tgcggtcggc gtgctcgaac accacgtcca tgtcatgctc ggtgaagagc acgccgatcg
     1681 actgctgccg cgcgatttgc gcggtgagcc gcatcagttc gatgcgctcg cgcggcgcca
     1741 tgccggcggt cggctcatcc atcaggagca gttttggctg attggcgagc gcgatcgcga
     1801 gctccagccg cttgagatcg ccataagcga gttcgccgca cggacgctcc gcatacccgc
     1861 ccatcccgac cagctccagc agacgaccgg cctcctctcg tgcggaccgc ggcgtcgagg
     1921 cccggagatt gaacaactgc cggcgatacg acaccagcgc gacctgaacg ttctcacgca
     1981 cggtcatggt cggaaacgtc gcggtgatct ggaaggtgcg tccgacgccg agccgccaga
     2041 tcgcgcgtgg tttcttgccc gcggtgtcct ggccgagcag gttgatccgg ccgctgtcgg
     2101 gaatattttg gccgttgagc atatcgaagc atgtgctttt tccggcgccg ttgggaccga
     2161 tcagcgccag gatttcgcca gccttgagcg cgaacgacac cttgcgcacc gcatggacgc
     2221 cgccatagga tttgctcaag tcctcgactg ccagcagtgt tggcgcgata ctcattcggc
     2281 ggtctcgatc ccgggcgcga gtgcaggctt ggacgattca ggcttgggcg cttcacttcg
     2341 gcggcgaagc ctgatcgttt ccagcatgcc gacgatgcct ttggggaacg ccaccacgat
     2401 cagaacgatg acgccgccga gcaccagctt ggacagatcg gtctggctca ccagccagat
     2461 cgagagcgcc ttgtagacga tggcgccgac gacagcgccg gagaccgttt cgacgccgcc
     2521 gagcagcacc atcacaagcg catcgaccga gagcgagata ccgaggctgt cgggaaagac
     2581 gctgcctttc aaataggcga acagcgcacc ggcaatgccg gcgacggtgc cggcaatcac
     2641 aaaggcggtc cactggatgc gcttgccgtc gatgccgatg gcctcgctgc gcagcggcga
     2701 gtcgcgcgtc gcccgcagcg cataaccgaa cggcgagaat acgatgatcc gaagggctgc
     2761 gaccgcaagc gcggccaccc cgatcgaaag ccagtagaaa tgcgacggcg ccgccgccca
     2821 cttttcgggc cagacgccga ggatgccgtt gtcgccgccg gtcaccgcga cccactggaa
     2881 cgcgatcgac cagacgatct gcgcgaatgc cagcgtcagc atcgcaaaat agacgccgga
     2941 cagttgaacc gcgaagaacc cgaacaccgc cgcgcccaga caccccaaca gcggaccgag
     3001 caaaagtgac aaaatcatcg gcaagccggc gagcttgccg agcaacgcaa caccataggc
     3061 gccgagcccg aaatacgccg cgtggccgaa tgaggcaagg ccgccgaccg acatcaggaa
     3121 atgcaggctc gcggcaaaga tcacgaagat cgcaatctcc gaaccgaccg tgagcgcata
     3181 gttgccgccg atcagcggca gcgccgcggc cagtaccagc gccgccatcg ccgcaagg
//
LOCUS       DASWGM010000961         7294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2206943,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000961 DASWGM010000000
VERSION     DASWGM010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206943"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /gene="ppx"
                     /locus_tag="VGA15_25860"
     CDS             <1..1501
                     /gene="ppx"
                     /locus_tag="VGA15_25860"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEX9591154.1"
                     /translation="KRPRKRASSVAVIDIGSNSVRLVVYESLTRSLVTLFNEKTLCGL
                     GREVQTTGLLAPDAVAKALTSLRRFRALCRVMKVGRVHAIATAACRDASNGPDFIAKA
                     ERICGVHIEILSGPREAKLSALGVVSGVHHPDGIVGDLGGGSLELIDVRGNRVHSGVT
                     LPLGSLALQDAAHGSLKRAERIVKTDLSGVAHLKAGRGRAFYAVGGTWRALARIHIIQ
                     SGYPLRVMHGYSIPAADALGFAQRLRRLAAANMLANIEVVADARRPLLAYAALVLEYI
                     IRVARPKTIVFSTFGVREGLLYERLPPAERSKDGLICAAQTLNELLSRSARHAQELIE
                     WTDRLVRVVRLRETDEDRRLRHAACLLSDIGWRVHPDHRGEETLNLITNGNFGAIGHQ
                     GRAFIALSVFYRYAGLSEQNEPPALVRELVPPAMVERARVLGAAFRVAHLISAARPGV
                     LPAIHFRSQGRKLMLVFEHRIVDLVADRVGSRFRQLARLVGRSGSIVRR"
     gene            complement(1634..2503)
                     /locus_tag="VGA15_25865"
     CDS             complement(1634..2503)
                     /locus_tag="VGA15_25865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX9591155.1"
                     /translation="MNWVLHILDPATIGALTAEFQSEMQQPAFWVAVGKIVWINVLLS
                     GDNALVIALACRGLAPRQRFWGMILGAGVAVILRIIFTGIVATLMELPYLKLVGGLAL
                     VVIAAKLLVPEEEDEDGVESASHLWAAVQIVVVADIVMSLDNVIAVAAAANGSVPLLI
                     LGLVISIPLIVAGAALIMALLNRLPILVWAGAALLGWIAGDVIATDPAIHPMLQALFA
                     GPIGVKLDVLMASFGTAPHFANGGNVGEVVCAVLGVIIVLIAGSIWRMRRLRNAKHGA
                     PSAGEAAQPIDRL"
     gene            complement(2539..2961)
                     /gene="ndk"
                     /locus_tag="VGA15_25870"
     CDS             complement(2539..2961)
                     /gene="ndk"
                     /locus_tag="VGA15_25870"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX9591156.1"
                     /translation="MAIERTFSIIKPDATKRNLTGAINALIEKAGLRIVAQKRIRMTR
                     EQAETFYAVHKARPFFGELVDFMISAPVVVQVLEGENAVLKHRDVMGATDPSKAADGT
                     IRKLHAKSIGENSVHGSDAPETAAIEIAQFFSGNEIVG"
     gene            3248..3517
                     /locus_tag="VGA15_25875"
     CDS             3248..3517
                     /locus_tag="VGA15_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur globule protein precursor"
                     /protein_id="HEX9591157.1"
                     /translation="MLRKLSLVAVAAASLGAAALAPTSASAWGGGWHGGWHGGWHDGW
                     HGRGWGGPRFYVGGPAYGYGYGGCYVRRLVPTPWGPRWRLVNRCY"
     gene            3726..3917
                     /locus_tag="VGA15_25880"
     CDS             3726..3917
                     /locus_tag="VGA15_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591158.1"
                     /translation="MNSRFSDNSEQIDRKTSRSICDAVGERLRQNLPALSSRLSSHLQ
                     HLLDELRKRDSDGRPMSSN"
     gene            4008..5885
                     /locus_tag="VGA15_25885"
     CDS             4008..5885
                     /locus_tag="VGA15_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX9591159.1"
                     /translation="MLSITDISVRIAGRLLIDNSSAQIVPGARVGFVGRNGVGKSTLF
                     HAIRGELPTESGSITIPPRWRVGSLAQEAPDGPESLIDVVLKADLERQALLHEAETAH
                     DPTRIADIQTRLVDIDAHSAPARAAAILSGLGFSSADQARSCSEFSGGWRMRVALAAT
                     LFAAPDLLLLDEPTNYLDLEGTLWLEDHLANYPRTVIVISHDRDLLDTSVDQILHLDR
                     GKLTLYKGTYSSFEEQRATREMLDAKHAKRQADERKRLQAFVDRFKAKASKARQAQSR
                     VKMLERMKPVTALVTQDVREISFPVPEKMLSPPIIAVDDVSVGYDPRKPVLNRVTLRI
                     DTDDRIALLGSNGNGKSTLVKLLANKLAPFSGHVTRAEKLSVGYFAQHQVDELNLDGS
                     PYDHVRKLMPDATETKVRGRTGAIGFSGKAGDTVVKSLSGGEKARLLLGLATFFGPNM
                     IILDEPTNHLDIDSRAALAEAINEFPGAVIMVSHDRYLIEACADQLWVVADHAVTTYD
                     GDLDDYKRMVLSARAMRANSRDRAGNERGAARDKPVRDKSEKRVPLKQKISDAEAEIA
                     RINGIISKIDTALALPDLFKRDPKQAAQLGKARAGAASALQRAEEEWLAASSQYDEAT
                     G"
     gene            complement(5923..6432)
                     /locus_tag="VGA15_25890"
     CDS             complement(5923..6432)
                     /locus_tag="VGA15_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591160.1"
                     /translation="MIVGCSGASDMFQSDLLSKDAEWFSRSGRLFIRNVSIETPPLSP
                     DKPVTSEDLVSADGLCPGMAPPGGPADANALTNGAAGAPAPTTTGTVALGHTECAVVR
                     GIGAPENVNLSSNARGDRVAVINYSRGQRAGIYSFTAGRLTSIERGAEPVAQPKAAKS
                     RAKKKPAAT"
     gene            complement(6617..7069)
                     /locus_tag="VGA15_25895"
     CDS             complement(6617..7069)
                     /locus_tag="VGA15_25895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX9591161.1"
                     /translation="MTEVLFYHLQNMSLDSVLPPLLEKSLERGWRVVVQSTSQERADA
                     LDAHLWTYRDDSFLPHATWRAGDAQDQPIILAVEEGNPNRANVRFLIDNAALPGDAGT
                     YERVVLVFNGDDGDALAAARGAWTDCKSRGFEVTYWQADEHGRWQRRQ"
     gene            complement(7095..>7294)
                     /locus_tag="VGA15_25900"
     CDS             complement(7095..>7294)
                     /locus_tag="VGA15_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX9591162.1"
                     /translation="GTRNGGSITAAQFLQRFVDDTPWAHLDIAGTAMGAPKSEINHSW
                     GSGFGVRLLERLVAEYYEARK"
BASE COUNT         1401 a         2342 c         2175 g         1376 t
ORIGIN      
        1 gaagcggccg cgcaaacgcg catccagcgt cgcggtcatc gacatcggct cgaattcggt
       61 tcgtctcgtc gtctatgaat cgctgacgcg cagcctcgtc accctcttca acgagaaaac
      121 cctttgcggg ctcggccggg aagttcaaac caccggcctg ctggcgcccg atgccgtcgc
      181 caaggctttg acatcgttgc ggcgttttcg cgcgttgtgc cgcgtgatga aggtcggtcg
      241 tgtccacgcg atcgccaccg ccgcgtgccg cgacgccagc aacggaccgg acttcatcgc
      301 caaggcggag cgcatctgcg gcgtccacat cgagatcctg tcgggtccgc gcgaagcgaa
      361 attatcggcg ctcggcgttg tatcgggcgt tcatcatccg gacggtatcg tcggcgatct
      421 cggtggcggt tcgctggaac tgatcgacgt ccgtggcaac cgcgtccaca gcggcgtgac
      481 gctcccgctc ggaagcctgg cgttgcagga tgcagcgcat ggatcgctga agcgcgccga
      541 gcgcatcgtc aagaccgatc tgtccggcgt cgcccacctc aaggccggcc gtggccgcgc
      601 cttctatgcg gtcgggggaa catggcgagc gctggcgcgc attcacatca tccaaagcgg
      661 ttacccgctg cgcgtcatgc acggctattc gattccggcg gcggacgcgc tcggtttcgc
      721 tcagcgcctg cgccggctcg ccgcggcgaa catgctggcg aacatcgaag tcgttgccga
      781 cgcacgacgg ccgttgctgg cctatgccgc cctcgttctc gaatatatca ttcgcgttgc
      841 caggccgaag accatcgtgt tttcaacttt cggggtacgc gaaggcctgc tgtacgagag
      901 gctgcctccg gccgaacggt ccaaggatgg attgatctgc gccgcgcaga ccctgaacga
      961 gttgctgtcg cgatcggcgc gccatgccca ggaattgatc gaatggaccg atcgtctggt
     1021 gcgggtcgta aggttgcggg aaaccgacga ggatcgccgc ctgcgccatg ccgcttgtct
     1081 gctgtcggac atcggatggc gggtacatcc cgaccaccgc ggcgaggaaa cgctcaatct
     1141 cattaccaat ggaaatttcg gcgcgatcgg ccatcaaggc cgcgccttta tcgcgctctc
     1201 ggtgttttat cgttatgccg gcctgagcga acaaaatgag ccgccggcgc tggttcgcga
     1261 actggtgccg ccggccatgg tggagcgtgc ccgcgtgctc ggcgcggcgt ttcgcgtcgc
     1321 ccatctgata tcggcggcgc ggcccggcgt tttgccggcg atccattttc gaagccaggg
     1381 ccgcaagctg atgctggtgt tcgagcacag aatagtcgat ctcgtcgccg accgtgtcgg
     1441 cagccgtttc aggcaattgg cgaggctggt cggccgctcc ggatcgattg taaggcgtta
     1501 aaattgtcgc ctcaaaactg aggtcacttg tctacagcct tttccgtttc gatagaatcg
     1561 aaacggggct ctagattctt gatttgacgc gttttcttgg cgcgaaccgg tctccacttc
     1621 gctcgaaaac gctctacagc cgatcaatcg gctgagcagc ctcgccagca gacggcgcgc
     1681 cgtgtttggc attgcgcagc cggcgcatgc gccagatcga tccggcgatc agcacgatga
     1741 tgacgcccag cacggcgcaa accacctcgc cgacgttgcc tccgttcgca aaatgcggcg
     1801 cggtcccgaa cgaagccatt aatacatcga gtttgacgcc gatcggacca gcgaaaagag
     1861 cctgtaacat cggatgaatt gccggatcgg tcgcaatgac gtcgccggcg atccagccca
     1921 gcagggctgc accggcccat accaaaatcg gcagacgatt gagcaacgcc atgatcagcg
     1981 cagcgccggc aacgatcaag ggaatgctga tcaccaggcc gagtatcagc agcggaacgc
     2041 tgccattggc ggcggcggca accgcgatga cgttgtcgag gctcatcacg atgtccgcga
     2101 ccaccacgat ctgcaccgca gcccacaggt gcgatgcgga ctctacgcca tcctcatcct
     2161 cttcttcggg caccagcaat ttcgcggcga tcacgactag cgccagtccg ccgaccagtt
     2221 tgaggtaagg cagctccatc aatgtcgcga cgataccggt gaagataatg cgcagaatga
     2281 cggcaacgcc ggcgcccaga atcatgcccc aaaagcgctg acgcggcgcc agtccacgac
     2341 aggccagcgc aatcaccagc gcgttgtcgc ctgacagcag cacgttgatc cagacgatct
     2401 tgccgaccgc gacccaaaac gccggctgct gcatttcgct ttgaaactca gcggtcaacg
     2461 caccgatcgt cgccggatcc aagatgtgca gcacccagtt cacaacgcaa tcctctcggc
     2521 gggcagctgc cgtccaaatc agccgacgat ttcgttgccc gagaaaaact gggcgatttc
     2581 gatggccgcc gtctcggggg catcggaacc atgcaccgag ttttcgccga tcgatttggc
     2641 gtgcagcttg cggatcgttc catccgcggc cttggaagga tcggttgcgc ccatgacgtc
     2701 gcggtgcttg aggacggcgt tttcaccttc gagaacctga accacaaccg gggcggaaat
     2761 catgaaatcc accagctcgc cgaaaaacgg acgcgccttg tgaacggcat agaaggtttc
     2821 ggcctgctcg cgggtcatgc gaatgcgctt ctgggcgacg atccgaagtc cggccttctc
     2881 gatcagcgcg ttgatcgcgc ccgtcagatt gcgcttcgtc gcgtccggct tgataatcga
     2941 gaaggtgcgt tcgatcgcca tgatttcttc cttgaaatgc gctgatggga gttgcggcgc
     3001 ttatatcggc gccatcgcgt aacggcaagc gatccggccc aatgccgcgg atgtccggcg
     3061 gccgattgac acccggaatt acaacaattt cacgaacatg aacccagtct gaaaccagca
     3121 tcccggtttc gttcagcttc tcaaggctag gcttcaatca accgaacgcc tcacgggttt
     3181 tggggggcgg cacgggtctg cagcttcacc catggcgccg atcgcgccga accttaagga
     3241 gacgaccatg ttacgcaaac tctcccttgt tgcagttgcc gcggcttcgt tgggcgcggc
     3301 cgcgttggcg ccgacttcgg cctccgcctg gggcggcggc tggcacggtg gctggcatgg
     3361 cggctggcac gatggctggc atggccgcgg ctggggcgga ccgcgcttct acgtcggtgg
     3421 cccggcttac ggttacggtt acggcggctg ctatgtgcgc cgactggtcc cgacgccgtg
     3481 gggcccccgc tggcgcctgg tgaaccgctg ctattgatct gaagtcgtcc gatccctgac
     3541 tccaaaaaag gccccggcgc cgtcgtgccg gggccttttc gctgattccg atcaggtgaa
     3601 accatccact ttcattcaat ctttactgaa aatgcacgca ttcggcgagc gacgcgaaac
     3661 aattcccatc cccatgactt ggtcctgcga cacccccttg aaattgacac gaagcggaac
     3721 ccgtcatgaa cagtcgtttc agcgacaaca gcgaacaaat cgatcgcaag accagccgat
     3781 cgatctgcga tgccgtcggc gaacggctgc ggcagaacct tcccgcgcta tcctcgcgcc
     3841 tctcgtccca cctccagcat ctgttggatg aattgcgcaa gcgggacagt gacggccgcc
     3901 cgatgtcatc aaactaaggt gaatattgag gcataagccg ttgcggaggc gctatttcgt
     3961 tcaaataaac cggttgcgtt ttctggccgc tccggtgaaa agcccgcatg ctttcaatca
     4021 ccgacatttc ggtccggatt gccggacggc ttcttatcga taatagctca gcgcagatcg
     4081 tgccgggcgc ccgcgtcggc tttgtcggcc gtaacggggt cggcaaatcg acgctgtttc
     4141 acgcgatccg cggtgaatta ccaaccgaat ccggctcgat cacgatccct ccgcgctggc
     4201 gggtcggcag tctcgcgcag gaagcccccg acggtccgga aagcctgatc gatgtcgtgc
     4261 tgaaggcgga tctcgagcgc caggcgctgc tgcacgaagc cgagactgcc cacgatccca
     4321 cccgcatcgc cgacattcaa acccggctcg tggacatcga cgcgcattcc gcccctgccc
     4381 gcgccgccgc gatcctcagc ggcctcggat tttccagcgc cgatcaggcc cgatcctgtt
     4441 cggagttttc cggtggctgg cggatgcgcg tcgcgctcgc cgcgacattg ttcgcggcac
     4501 ccgatctttt gctgttggac gagcccacca actatctcga tctcgaaggc acgctgtggc
     4561 ttgaggatca tctggcgaac tatccgcgca ccgtgattgt catcagccac gaccgcgacc
     4621 tgctcgacac ctcggtcgac cagatcctgc acctcgatcg cggcaagttg acactctaca
     4681 aggggaccta ctcctcgttc gaggaacaac gcgccacgcg cgaaatgctg gacgccaagc
     4741 acgccaaacg gcaggccgac gagcgcaagc ggctgcaggc ctttgtcgat cgtttcaagg
     4801 ccaaggcgtc gaaagcccgc caggcgcagt cccgcgtcaa aatgctggaa aggatgaagc
     4861 cggttaccgc gctggtgacg caggacgtgc gcgagatttc atttccggtg cctgagaaga
     4921 tgctgtcgcc gccgatcatc gcggtcgatg atgtctctgt cggctacgat cccagaaagc
     4981 ccgtgctcaa tcgcgtgacg ctgcggatcg acaccgacga ccgcatcgcc ttgctcggct
     5041 ccaacggcaa tggcaagtcg acgctggtaa agctgcttgc caacaaactg gcgccgtttt
     5101 ccggtcacgt cacccgcgcc gagaaactgt cggtcggata tttcgctcag caccaggtcg
     5161 acgagctcaa tctcgacgga tcgccctacg atcacgttcg caagctgatg ccggatgcta
     5221 ctgagaccaa ggtgcgcggg cgcaccggcg cgatcggctt ctccggcaag gctggcgaca
     5281 ccgtggtcaa aagtttgtcg ggcggcgaaa aagcccggct gctgctggga ctggcgacct
     5341 tcttcggtcc caacatgatc attctcgatg agccgaccaa ccatctggac atcgacagcc
     5401 gtgctgccct tgccgaggcg atcaacgagt ttcccggcgc ggtcatcatg gtctcccatg
     5461 atcgctatct gatcgaagcc tgcgccgatc aattatgggt ggtcgccgat catgcagtca
     5521 cgacctatga cggcgacctc gacgattaca agcgcatggt gttgtcggcg cgcgccatgc
     5581 gcgccaattc gcgcgaccgc gccggcaatg agcgcggagc agcccgcgac aagcctgtcc
     5641 gcgacaaaag cgaaaagcgc gtgccgctga agcagaagat ttccgacgcc gaggccgaga
     5701 tcgcgcgcat caacggcatc atctccaaga tcgataccgc gctggcgctg ccggatttgt
     5761 tcaagcgcga cccaaagcag gcagcgcaac tcggcaaggc gcgcgccggc gccgcgagcg
     5821 ccctgcagcg cgccgaggaa gaatggctgg cggcaagttc gcaatacgac gaagcaaccg
     5881 gctgagctga ttcgagactg atcgaagcgg gaagaattgc ggttacgtcg cggccggctt
     5941 cttcttcgct ctcgactttg cggcctttgg ctgcgccacc ggctcggcgc cgcgctcgat
     6001 cgatgtcagg cgcccggctg tgaaggaata gattccggcg cgctgtcccc gcgaatagtt
     6061 gataaccgcc acccggtcgc cgcgcgcatt gctcgacaga ttgacgttct caggtgcgcc
     6121 aataccgcgc acgacagcgc attcggtatg accgagcgcg accgttcccg ttgtcgtcgg
     6181 cgcaggcgcg ccagcagcgc cgttggtcaa ggcgttggcg tctgcggggc cgccgggagg
     6241 cgccatgccc gggcaaagcc cgtcggcgct gaccaaatcc tctgaagtaa ccggcttgtc
     6301 gggagatagc gggggcgttt caatcgaaac gtttcggatg aacagcctgc cggagcgcga
     6361 aaaccattcc gcatctttcg acagcagatc ggactgaaac atatcggacg cgcctgaaca
     6421 tcccacgatc atgggggcaa gcgccagcag cgccagcagt cgttttcgat gagaggtttt
     6481 gtctcgcacg atgaactaaa ttcccacccc aagcccaagc cgaagcgatt gttccactat
     6541 tactttgcgg cgcgaccgtg gcttggtgca ggcgcaccga gccgaaccac agattatcat
     6601 taatcgccgt gcgccgctat tgccgcctct gccaccggcc gtgctcgtcg gcctgccagt
     6661 aggtgacctc gaatccccgc gatttgcaat cggtccaggc gccccgcgcc gcagcaagcg
     6721 catcgccatc gtcgccgtta aagaccagaa ccacccgctc gtaggtgccg gcatcgccag
     6781 gcaaggcggc gttatcgatc aggaacctga cattcgcccg attcggattg ccctcctcga
     6841 ccgccagtat gatcggctga tcctgcgcgt cgccggcgcg ccaggtggcg tgcggcaaaa
     6901 acgaatcgtc gcgataggtc cataaatggg cgtcgagcgc gtcggcacgc tcctgcgacg
     6961 tcgactgcac cacaacgcgc cagccacgct ccagcgactt ttcaagcaac ggcggcaaaa
     7021 cgctgtcgag cgacatattt tgcaggtggt agaacaaaac ttccgtcatc cgcttttcag
     7081 ctatttggtt tcagttattt cctggcttcg taatactccg cgaccagccg ctccagcagg
     7141 cgaacgccaa agcccgatcc ccagctgtga ttgatctcgg atttcggagc gcccatcgcc
     7201 gttcccgcga tatcgagatg cgcccacggc gtgtcgtcga cgaagcgctg caggaactgc
     7261 gcggcggtaa tcgaaccacc gttgcgggtg ccgg
//
LOCUS       DASWGM010000962         6137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1482998,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000962 DASWGM010000000
VERSION     DASWGM010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482998"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VGA15_25905"
     CDS             <1..453
                     /locus_tag="VGA15_25905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX9591163.1"
                     /translation="IYALDPDVILIDLENPSRDVLEQMFQVSRAVRRPIAMFVDQSDA
                     ASIQASVDAGVSAYIVDGLKKERIKPILDLCISRFNAFSKLQDELDRAKSALEDRKVI
                     DRAKGILMKLKGLTEDEAYVLLRSTAMREKKKIGEIAQSILTASELLK"
     gene            450..1619
                     /locus_tag="VGA15_25910"
     CDS             450..1619
                     /locus_tag="VGA15_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9591164.1"
                     /translation="MTTPLRIGFIPLVDAAALIVAVDKGFAKAEGLDVTLVREVSWSN
                     VRDKLNIGLFDAAHLLAPVAIASSLGLGHVKVPIVASFNLGLNGNAITVSPALHAAIM
                     SEIEGDPFDPMATALALARVVAARRQSGAEPLTFGMTFPFSTHNYQLRFWMAAGRVDP
                     DEDIRMVVLPPPYMVDSLANGHVDAFCVGAPWNSVAVDLGVGHILHFVADILVRAAEK
                     VLATRQEWSEKNPDILTALIRAHQRAAEFIEHPHNRAEAAGILAQPERIGVDADVIQR
                     TLDGRLKISPDGTMRESSRYLLVGREGAARPDPVQAAWLYAQMVRWGQATISPEAVKI
                     AKAVFRPDLYDAALGLRAGEDEAAFGSVGAFAGPAFDPDDVEGYLAAFNVFRRVP"
     gene            1953..2813
                     /locus_tag="VGA15_25915"
     CDS             1953..2813
                     /locus_tag="VGA15_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX9591165.1"
                     /translation="MRAALELDMDYAKPADVVASMIDASLKKLALSPRDLLIRGALSG
                     ALLGAATALAFSGAITTGQPLVGALIFPVSLVMIVLLGLELVTGSFGLLPLARLEGKA
                     TWNATIANWSWVFLGNLLGSIAFGALLAISLTNMGKIEPAGVAARIISIAEAKTVANE
                     ALGAAGMVSVFVKAILCNWLVCLGVVMAMTSTSTVGKIAATWLPIFTFFALGFEHTVV
                     NMFIIPTGMLLGAKVNVYEWWVWNQIPVTLGNFVGGFVFTGLALYTTFKPVKPLALAA
                     SPIAAQVAAE"
     gene            2831..4621
                     /locus_tag="VGA15_25920"
     CDS             2831..4621
                     /locus_tag="VGA15_25920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HEX9591166.1"
                     /translation="MKLETTTADFSDEQKRYLEGFMSGLQVGRVGRLAGGAVPAGKAD
                     PEPMGPDAAGFKAQDKAIASGKKLADQEKFKREEHPFDAYSRLKQQALTNAPPTPADN
                     FRWRYYGLFYVAPAQDSYMCRLRIPNGILKHWQFAGVADLAESCGGGYSHVTTRANIQ
                     IREILPKHTVALLEGIQDLGLCSRGSGADNIRNVTGTPTAGIDPQELIDTREYARQWH
                     YHILNDRSLTGLPRKFNVAFDGAGRIAVLEDTNDIAFAAVEVKGGFGVDPGVWFRLGI
                     GGITGHKDFAKETGIIVKPADATKVADAIVRVFIDLGDRTNRLKARLKYVIDGMGVEK
                     FLVLVEEKLGHALTRVPPEALALRPAFDRMAHIGIHAQKQPGLNWIGVVLPVGKLTCD
                     QMRGLASIARDLGDGDIRLTVWQNLLISGVRDENVALATAAIETLGLAVKASHIRAGL
                     IACTGNAGCKFAASDTKRHAAEIGDWCETRVPIEAPLNIHLTGCHHSCAQHYISDIGL
                     LAAKVPAGSDDDTVEGYHLYAGGGFGPDADIGHEVYRDVKAEDAPKTVEKLLKAYLAN
                     RVSAEETFLTFSRRHDGEALRKLAEAEAMT"
     gene            4618..>6137
                     /locus_tag="VGA15_25925"
     CDS             4618..>6137
                     /locus_tag="VGA15_25925"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEX9591167.1"
                     /translation="MNQMTPPPRLEIIPPTAPFTEGQRSWLNGFLAGLLQLDGSTALA
                     PAQNGALLAPSGDGDDGEAPWHDQTMPIADRVKLAEGRPLRRRMMAAMAQQDCGQCGY
                     NCNDYSDAIVSKAEPRLNLCVPGGKETARMLKALNEELEKAPPASSGAAAVSAVAAPA
                     TAAEPGRSRDNPVPATFVSRRLLNASGSEKETWHIDFDLSGCGLDYVVGDSFGIFARN
                     DLGHVDQIIALLGALHTTEVRGKTLREVLRDDVSLAPAPDSLFELISFITGGAQREKA
                     RALAQGEDPDGDAATLDVMAALQKFSGVRPHPEAFVEALEPLQPRLYSISSSHNATPG
                     KLSLTVDCVRYVVGKRKRLGLASTFLAERIKPGDQLKVYVQKAHGFALPQDPKTPIIM
                     IGPGTGIAPFRAFLLDRRATGAPGKNWLFFGHQRSSCDFFYADELNAMKTSGLLTRMS
                     LAWSRDGDKKFYVQDRMREVGRELWTWLAEGANIYICGDAKRMAKDVERALVDIVA"
BASE COUNT         1155 a         1872 c         1933 g         1177 t
ORIGIN      
        1 atctatgcgc tcgatcccga cgtcatcctg atcgacctgg aaaatcccag ccgcgacgtt
       61 ctggagcaga tgttccaggt cagccgcgcg gtgcggcggc cgatcgcgat gttcgtggat
      121 cagagcgacg cggcatcgat ccaggcttcg gtcgatgccg gtgtctccgc ctatatcgtc
      181 gacggattga agaaggagcg catcaagccg atcctcgacc tctgcatttc gcgtttcaac
      241 gccttctcga agctgcagga tgaactcgat cgcgccaagt cagcgctcga agaccgcaag
      301 gtcatcgatc gcgccaaggg catcctgatg aagctgaagg ggctgaccga agacgaggcc
      361 tacgtcctgc tgcgatcgac ggcgatgcgc gaaaaaaaga aaatcggcga gatcgcccag
      421 tcgatcctga ccgcctcgga gttgctgaaa tgaccacacc gcttcgtata ggttttattc
      481 cgctggtcga cgccgcagcc ctgatcgtcg ccgtcgacaa ggggttcgcc aaggcggaag
      541 ggctcgatgt cacgctggtg cgggaagtgt cgtggtcgaa tgtccgcgac aagctcaaca
      601 tcggcctgtt cgacgccgcc cacctgctgg cgccggtcgc gatcgcgtcg agcctcgggc
      661 ttggtcacgt caaggtgccg atcgtcgcct ccttcaatct gggcctcaac ggcaacgcga
      721 tcacggtatc gccggcgctg catgcggcga tcatgagcga gatcgaaggc gatccgttcg
      781 atccgatggc gacggcgctg gcgctggcgc gggtcgtcgc ggcacggcgc cagagcggcg
      841 ccgagccgct gaccttcggc atgaccttcc cgttttcgac ccacaattat caattgcggt
      901 tctggatggc cgcgggcagg gtcgatcccg acgaggacat tcgtatggtg gtgctgccgc
      961 cgccttatat ggtcgacagt ctcgccaatg gtcacgtcga tgcgttctgc gtcggcgcgc
     1021 cctggaattc cgtcgcggtc gatctcggcg tcggacatat cctgcatttc gtcgcggata
     1081 ttctggtacg ggcggccgag aaggtgcttg cgacccggca ggaatggtcg gagaaaaatc
     1141 cggacatcct gaccgcgttg atccgcgcgc atcagcgcgc cgccgagttc atcgagcatc
     1201 cgcacaatcg cgccgaggcc gccggtattc tggcgcagcc ggagcggatc ggcgtcgatg
     1261 ccgatgttat ccagcgcacg ctcgatggcc gcctgaagat ttcccccgac ggcaccatgc
     1321 gcgaaagcag ccgctatctg ctggtggggc gggagggcgc ggcccggccc gatccggtgc
     1381 aggccgcctg gctttatgcg cagatggtcc gctgggggca ggccacgatc agccccgagg
     1441 ctgtcaagat agccaaggcg gtgttcaggc cggatctcta tgacgcggcg ttgggactgc
     1501 gcgcgggcga ggacgaagcc gctttcggtt ctgtcggcgc gttcgcaggg cctgcattcg
     1561 atccagatga cgtcgaggga tacctcgccg cattcaacgt ttttcgccga gttccataaa
     1621 gtggaacaac gcccgattaa atattaggcg gtttttccat tgtttatgtt ttaggcgttt
     1681 gcagccttat gtggcagctg ggctaaccct tatattgcgt cgcaatatcg gcaatccctt
     1741 gagcgagaag agctttccaa gaattgctgc actggcacac aacttgaata gggcaggata
     1801 agccagccgc cgtagatgcc cgctggcatc gtccaagatg gatttccgca gcaacgaggc
     1861 tgatcggatc gcttcgaaat ctccgatacg gcgctgcctt tcgaatcgaa tttcctagcg
     1921 atcacatgcc cctttcctcg ttgcagccac gcatgcgcgc ggcgctggag ctcgatatgg
     1981 actacgcaaa acctgccgat gttgtcgcct cgatgatcga tgccagtttg aagaaactgg
     2041 cgctttcgcc tcgggatctg ctcattcgcg gcgcgctttc cggtgcgctg ctgggtgctg
     2101 cgaccgcgct tgcattcagt ggcgccatca ctacgggtca gccgctggtc ggcgcactga
     2161 tctttcccgt cagtctcgtg atgatcgtgc tgctcgggct cgaactcgtg accggaagtt
     2221 ttggactgct gccgctggcg cggcttgagg gcaaggccac ctggaacgcc accatcgcga
     2281 actggtcgtg ggtgtttctt ggaaacctgc ttggcagcat cgccttcggc gcccttcttg
     2341 cgattagcct caccaatatg gggaagatcg aaccggcggg ggtcgcggcg cgcatcatca
     2401 gcattgccga ggcaaaaacc gtcgccaacg aggcgctcgg cgcggccgga atggtcagtg
     2461 tcttcgtcaa agccatcctc tgcaactggc tggtttgtct cggcgtcgtc atggcgatga
     2521 cgtcgacatc cacggtggga aagatcgccg cgacctggct gccgatcttc accttcttcg
     2581 cgctgggatt cgagcacacc gtcgtcaaca tgttcattat tccgaccggc atgctgcttg
     2641 gcgcgaaggt gaacgtttac gagtggtggg tgtggaatca gataccggtg acgctcggta
     2701 atttcgtcgg cggcttcgtg ttcaccggtt tggccctcta cacgaccttc aagccggtga
     2761 aaccgctggc gctggcggca tcgccgattg cagcgcaagt cgcggcagaa taacggcggc
     2821 gttgcgcatc atgaaacttg aaaccacaac cgccgatttt agcgacgagc agaagcgcta
     2881 tctggaaggc ttcatgtcgg gcctgcaggt ggggcgcgtg gggcgcctcg ccggtggtgc
     2941 cgtgccggcc ggcaaggccg atccggagcc gatgggtccg gacgccgcag gcttcaaagc
     3001 ccaggacaag gccattgcgt cgggcaaaaa gcttgcggat caggaaaagt tcaaacgcga
     3061 agagcatcca ttcgatgctt attcgcggct gaagcaacag gctctgacca acgcgccgcc
     3121 aacgccggcg gataattttc gctggcgcta ttacggcctg ttttatgtcg cgccggcgca
     3181 ggattcctac atgtgccggt tgcggattcc aaacggcatc ctcaagcatt ggcagtttgc
     3241 aggtgtcgcc gatctcgccg aaagctgcgg cggtggctac tcgcatgtca ccacccgcgc
     3301 caatatccag atcagggaga tcctgccgaa gcacaccgtg gcgttgctcg agggcatcca
     3361 ggatctcggc ctgtgctcgc gcggctccgg cgccgataac atccgcaacg tcacgggcac
     3421 gccgaccgcc gggatcgatc cgcaggaatt gatcgacacc cgcgaatacg cccggcaatg
     3481 gcactatcac attctcaacg accgctcgct gaccggattg ccgcgcaagt tcaatgttgc
     3541 cttcgacggc gccggcagga tcgcggtgct tgaagacacc aacgacatcg ccttcgccgc
     3601 ggtcgaggtg aagggcggct tcggggtcga tcccggcgtc tggtttcgcc tcggtattgg
     3661 aggcatcacc ggccacaagg attttgccaa ggagaccggc atcatcgtca aaccggcgga
     3721 tgccaccaaa gtggcggacg ccatcgtccg cgtgttcatc gatcttggcg accgtaccaa
     3781 ccggctcaag gcgcggttga aatatgtcat cgacggcatg ggcgtggaga aattcctggt
     3841 tctggtcgag gaaaagctgg gtcacgcctt gacccgggtg ccgccggaag ccctcgcgct
     3901 gcgcccggca ttcgatcgga tggcccatat cggtatccat gcccagaagc agccgggcct
     3961 gaactggatc ggcgtcgtgc tgccggtggg caaacttacc tgcgatcaaa tgcgcggcct
     4021 cgccagtata gcgcgggatc tcggcgacgg tgatatccgc ctcacggtgt ggcagaatct
     4081 gctgatatcg ggtgtgcgcg acgagaacgt agcgctggcg acggctgcga tcgagacgct
     4141 cggcctcgcg gtcaaggcgt cacacatacg cgccgggctg attgcctgca ccggcaatgc
     4201 cggctgcaag ttcgcagcct ccgataccaa acgccacgcg gccgagatcg gcgattggtg
     4261 cgagacacgc gtgccgatcg aagcgccgct gaatatccat ctcaccggct gtcatcattc
     4321 ctgcgcccag cattacatca gcgatatcgg gctgctcgcg gcaaaggtgc cggcgggaag
     4381 cgacgacgac acggtcgagg gttaccacct ttatgccggc ggcggtttcg gaccagatgc
     4441 cgatatcggg cacgaggtct atcgcgatgt gaaggcagag gatgcgccga agacggtgga
     4501 aaaattattg aaggcctatc tcgccaaccg tgtctcggct gaggagacgt ttctcacctt
     4561 ttcacgccgc catgacggcg aagccctgcg caagctggct gaagcggaag caatgacatg
     4621 aaccagatga cccccccgcc acggctcgaa atcattccgc caaccgcacc gttcaccgag
     4681 ggacagcgtt cgtggctgaa cggtttcctc gccgggctgc tgcagcttga cggttcaaca
     4741 gcgctggcgc cggcacagaa cggcgcattg cttgcgccgt ccggcgatgg cgacgatggc
     4801 gaagcgccgt ggcacgacca gaccatgccg attgccgatc gcgtgaagct tgccgaaggc
     4861 cgcccgttgc ggcgccgcat gatggcggcg atggcgcagc aggattgcgg ccagtgcggc
     4921 tacaactgca acgactattc ggatgcgatc gtcagcaagg ccgaaccgcg gctcaacctc
     4981 tgcgtacccg gcggcaagga aaccgcccgg atgctcaagg cgctgaacga ggaactggaa
     5041 aaggcgcctc cggcgtcgtc cggagcggca gcagtatccg cggtagcggc gcccgcgacc
     5101 gcggcggagc ccggacgttc gcgcgacaat ccggtgccgg cgaccttcgt gtcgcgccgc
     5161 ttgctgaacg caagcggatc ggaaaaggaa acctggcaca tcgatttcga tctcagcggt
     5221 tgcggcctcg actatgtcgt cggcgactcc ttcggcattt ttgcgcgtaa cgatctgggc
     5281 cacgtcgatc agatcattgc cttgctaggc gccttgcaca cgacggaggt tagaggcaag
     5341 acccttcgcg aggtattgcg cgacgatgtt tcgctggcgc ccgcgccgga ttcgctgttc
     5401 gaactgatct cgttcatcac cggcggcgcg cagcgcgaga aagcgcgcgc gctggcgcaa
     5461 ggcgaggatc ccgatggcga cgccgcgacg ctggatgtga tggccgcgct gcaaaaattt
     5521 tccggcgtgc gtccgcatcc cgaagcgttt gtcgaagcgc tggagccgct gcagccacgg
     5581 ctctattcga tctcctcgtc gcacaacgcc acacccggca agctgtcgct gacggtagac
     5641 tgcgtgcgct atgtcgtcgg caagcgcaag cgcctcggac tagcctcgac ctttctggcc
     5701 gaacgcatca agccgggcga tcagctgaaa gtctatgtgc agaaggccca cggctttgcg
     5761 ctgccgcagg atccaaagac gccgatcatc atgatcgggc caggcaccgg tatcgcaccg
     5821 ttccgcgcct ttctgctcga ccgccgcgcc accggcgcgc caggcaagaa ctggctgttc
     5881 ttcggtcatc agcgcagcag ttgcgatttc ttctatgccg acgagttgaa tgccatgaaa
     5941 acgtcaggtc tgctgacgcg aatgtcgctg gcgtggtcgc gcgacggtga caagaaattc
     6001 tatgtgcagg accgcatgcg cgaggtcggc cgcgaattat ggacgtggct cgccgaaggc
     6061 gccaacatct atatctgcgg cgatgccaaa aggatggcaa aagatgtcga gcgcgcgctg
     6121 gtcgatatcg tcgcgca
//
LOCUS       DASWGM010000963         7256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1342639,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000963 DASWGM010000000
VERSION     DASWGM010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342639"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGA15_25930"
     CDS             <1..187
                     /locus_tag="VGA15_25930"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEX9591168.1"
                     /translation="ASVCGPLITAIGNDRELRRKLQQGSQQLQGEVLELALEDLISQT
                     FPSDLVQLYQKLSMART"
     gene            363..659
                     /locus_tag="VGA15_25935"
     CDS             363..659
                     /locus_tag="VGA15_25935"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HEX9591169.1"
                     /translation="MKQLLKIAAAPAAAAFALAWVAMATPAAASPGEYCRIEYGSGGI
                     RLCSFATMEQCRAAVSGRSGVCERDPYLPNNNSAFAYQPKHPLAKRVRQPVVNQ"
     gene            complement(840..2465)
                     /locus_tag="VGA15_25940"
     CDS             complement(840..2465)
                     /locus_tag="VGA15_25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEX9591170.1"
                     /translation="MLAGLATISAAPAATVMMEDAASACGGLTGSSDAVRIDSVTLQT
                     PSPLAVSERAPTPAARIAPANPAFCKVLGHIEPTDTKAPPIKLEVNLPVEWNGRSVQY
                     GGGGFNGTLITGLGLPPAYPFDKPSPLARGFVTYGTDSGHESKPGEPPQVFALNDEAF
                     ENFAYRSYKKVRDAAVALMVRAYGKPPEKMYFMGSSEGGREALTMAQRYPDDFDGILA
                     RVPVINWVGLQHAGTRSGLATMGDGWIRPAQVKLVADAVRQACDKADGSDDALVQDPV
                     GCKAAFKAESLCCVGGQSGDQCLTDAQIKAVDTLHATYKFPFPLANDLDDYPGWGVSG
                     EDTPSFGPTGGWTAWWLGTAAPAQPPTPNNGIAWVYGAGGIQYVFARDPKLDVTTYKP
                     EDHKARVLEVSQLMDSTNPDLGRFRARGGRLIVLEHMADYAQSPYAGIRYFENVQRKL
                     GKVETAEFARLYTAPGVDHVGSGAPANVDMLSALVDWVEKGHAPGDLEVVEQKVEAPA
                     FAVVRALPLCRWPAWPHYKSGPVTAASSFSCAP"
     gene            complement(3054..4088)
                     /locus_tag="VGA15_25945"
     CDS             complement(3054..4088)
                     /locus_tag="VGA15_25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9591171.1"
                     /translation="MQYRNLGASGLQVPVLSFGTGTFGGEGPLFSAWGNSSADEARRL
                     IDICLDAGVTLFDTADVYSNGGSEERLGAAIKGRRDRLMISSKTSLPMGDGPADAGSS
                     RQRLTRAVDASLTRLGTDHIDLLQLHAFDAGTPIDEVLYTLDMLVRAGKVNYVGVSNF
                     SGWQLMKSLGIAAQNGWPRYAAHQVYYSLIGRDYEWELMPLGLDQGVGALVWSPLGWG
                     RLTGKIRRGQPLPEASRLHATAQFGPPVSNERLYTVTDALEIVAGETGKSIPQVALAW
                     LLTRPTVSSVIIGARNEVQLRDNLGAVGWSLTTGQIARLNTASDVMSAYPYYPYRTQQ
                     GFARLNPPPV"
     gene            complement(4110..5279)
                     /locus_tag="VGA15_25950"
     CDS             complement(4110..5279)
                     /locus_tag="VGA15_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9591172.1"
                     /translation="MPIAVYALTVGAFGIGTTEFIIMGLLLQVAADMHVGVSTAGLLI
                     SGYALGVFVGAPLLTLASRRMPRKAVLLALMAIFTLGNATCALAPSYGWLMAARVLTS
                     LAHGTFFGVGSVVATGLVAQNKRASAISTMFVGLTVATLLGVPFGAWLGLLLGWRAAF
                     WAVAAIGVIAFAILAVSVPGHLGRDDEPTSLRDEVGVLARPQVLLGLAMTVFGFAGLF
                     TVFTYIQPILTRISGFSQSAVSPVLLVLGAGLAVGNVMGGRFADRGLARALIGTLAVL
                     AIVLGALGPALGTKIPTVLLVGLLGVAAFATVAPLQLRVLEKAGSAGQNLASSLNIAA
                     FNLGNALGAWAGGVTIDAGLGFAALPLVAAAITLLGLALALWSVQLDRRLVSATA"
     gene            5383..6351
                     /locus_tag="VGA15_25955"
     CDS             5383..6351
                     /locus_tag="VGA15_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9591173.1"
                     /translation="MPRLDTNRAAEMEVFVRVVDLGGFTAAAREFRLTPSGVSKLVSR
                     LESRLDSRLVNRSTRRLQLTPEGQVFYDRAVRVLADLDEAEREASAGACPRGHLRVNS
                     NIPFGMRHIMPLVPAFLALNPEVTLDLVFSDIVVDLMQERADVAIRVGPLRDSALLAR
                     KLGASRMAVVATPEYLAKSGTPANPDDLAKHKGVGWTFARSIGGWPFLSGKATVEVSP
                     PVARASDGEAARALALGGVGLARLALFHIRPDIEAGRLIPVLQDFNPGDCEDIHAVFL
                     GQGGPLPARVRAFIDFLGENIRLDDLDLERTRNDEWRVRSARARLR"
     gene            complement(6402..6677)
                     /locus_tag="VGA15_25960"
     CDS             complement(6402..6677)
                     /locus_tag="VGA15_25960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591174.1"
                     /translation="MKPDRKLKRPSASPVARPRLGPMYSVIGGDTAFGAKWTFALGYD
                     FRGFQRDLADFTTTTPELKLSVHTAFITLSGTGRPIAVAWPRPGLAL"
     gene            6820..>7256
                     /locus_tag="VGA15_25965"
     CDS             6820..>7256
                     /locus_tag="VGA15_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEX9591175.1"
                     /translation="MLKSIDPILNADVLYALRAMGHGDELVICDTNFPADATARQTVL
                     GRLLRIDNVTAGRAARAILSVMPLDTFVDKPALRMQIVGRPDEVPPVQIEVQREIDAA
                     QGGPLSMGSIERFAFYELAKKSYCVIQTGERRFYGCFVFKKGV"
BASE COUNT         1388 a         2295 c         2289 g         1284 t
ORIGIN      
        1 cgcatcggta tgtggtccgc tgatcaccgc cataggaaat gacagagaac ttcggcgtaa
       61 gcttcagcaa ggctcgcaac agcttcaggg agaagtactc gaactggctc tggaagacct
      121 gatttcgcaa acgttcccat cggatctggt ccagctgtac caaaagttat caatggcgcg
      181 gacataatac agcgtgttca gagtcaaaat acagaaaatc aaggacgacc agcggcaggc
      241 caaagccgac attgcctctt cgaaccggac atgcagatct cccgcatccg gctctcggac
      301 tctgacatcc catgtgtgaa atcttgggac tgaacccaac acacacacac agagagagaa
      361 agatgaaaca gcttttgaaa atagccgccg cgcccgccgc tgcggcgttc gcgctggctt
      421 gggttgccat ggcgacaccc gctgccgcga gcccgggcga gtactgccgc atcgagtacg
      481 gttcgggcgg aatacgactc tgcagcttcg cgaccatgga gcaatgtcgg gcggcggtct
      541 ccggacgcag cggcgtttgt gagcgcgatc cgtacctgcc caacaacaac agcgcctttg
      601 cctatcagcc gaagcacccg cttgcgaaga gggtcaggca acctgttgtg aatcaatagg
      661 gccgtatctt cgcagaaacg gtttcgtcgt ggtcgggcac cagcgcaaga gtgcgtcttc
      721 gcgctggacg tcccggccat ccacgtcttt cttgctgcgc gatcccaaga cgtggatgcc
      781 cggcaaggcc gggcatggcg ggttctatca gcgaaagacg catgaagttc gcgattgctt
      841 cacggcgcgc atgaaaagct cgacgcggca gttacagggc ccgacttgta gtgcggccag
      901 gccggccatc tgcacaacgg caacgcacgg accacagcaa acgcaggcgc ttcgaccttc
      961 tgctcgacaa cttcgagatc gcccggcgcg tggcccttct cgacccaatc gaccagcgcg
     1021 ctcagcatgt cgacattcgc cggcgcgccg gaaccgacat ggtcgactcc aggtgccgtg
     1081 tagagccttg cgaactccgc cgtctccacc ttgcccagct tgcgctggac gttttcgaaa
     1141 tagcggatgc cggcataggg gctttgcgca tagtcggcca tatgttcgag cacgatcaac
     1201 ctgccgcccc gcgcgcggaa gcggccgaga tccggattgg tcgagtccat gagctgcgac
     1261 acttcgagga ctcgcgcctt gtggtcttcc ggcttatagg tcgtgacatc gagcttggga
     1321 tcgcgcgcga agacatattg aatgccgccg gcaccgtaga cccaggcgat gccgttgttt
     1381 ggcgtcggcg gctgcgcggg cgccgccgtg cccagccacc aagcggtcca tccgccggtc
     1441 ggaccgaaag acggcgtgtc ttcgcccgac acgccccagc ccggataatc atcaaggtca
     1501 ttggcgagtg ggaacgggaa tttatatgtc gcgtgcagcg tgtcgactgc cttgatctgc
     1561 gcgtccgtca ggcactgatc gccactctga ccgccgacgc aacacagcga ctctgccttg
     1621 aatgccgcct tgcagcccac ggggtcctga accagcgcat cgtccgagcc gtcggccttg
     1681 tcgcaggcct gccgaacggc gtcggcgacg agcttcacct gggccggacg gatccagcca
     1741 tcgcccatgg tcgcgagtcc cgaccgcgtg cctgcatgtt gcagtccaac ccagttgatg
     1801 acgggcacgc gcgcaaggat gccgtcaaaa tcatccgggt agcgttgcgc catggtcagc
     1861 gcctcgcggc cgccttcgga tgatcccatg aaatacattt tctccggcgg cttgccatag
     1921 gcgcgcacca tcaaggcaac ggcggcatcg cgcactttct tatatgaacg gtacgcgaaa
     1981 ttctcgaaag cttcgtcgtt gagcgcaaaa acttgcggcg gctcgccggg cttgctctcg
     2041 tggccggaat ccgtgccgta ggtaacgaag ccgcgcgcga gcggcgacgg cttgtcgaaa
     2101 ggataggccg gcggcaggcc gagcccggtg atgagagtgc cgttgaagcc gcctccgcca
     2161 tattgaacag agcggccgtt ccattcgacc ggcaggttca cctcgagctt gatcggcggc
     2221 gccttggtat cggtcggctc gatatggccg agcaccttgc agaacgcggg gttggccggc
     2281 gcgatccgcg ctgccggcgt cggcgcgcgt tcagacactg caagcggaga aggcgtctgc
     2341 agcgtcacgg aatcgatccg cacggcatcc gaggatccgg taaggccgcc gcaggccgaa
     2401 gcggcatctt ccatcatcac cgtcgccgcg ggcgcggcac tgatggttgc cagccccgca
     2461 agcatgcttg cgagccacac acgcggatcg gcatgaaatc gcaccatcgt ttccacccgg
     2521 cttgtcgatt attgttcggg ccgaccggcg acgctttcga tgcgaccgcc tgtgggtatc
     2581 catcattgcg gcgatcgccg tcgccacgga tcaatatcgc acgacctttt tccactgtcc
     2641 aacgggctcg tcgtagatcg gcgggttgaa tgtcgcccga tccaccaggc cggacgtgaa
     2701 ggcgcagtgc cggtgctagt cgaatgattt taatctgagt acgtggtcac gtgaaaatct
     2761 tccgtcggga cgtcgctgtt tgcgtacgaa cggccgcttt gcgccaaaac cagccgttcc
     2821 gggaaatcgg cctttcgacc tggtacgaac ccgatcagtt gggctgtcct tcggcttgaa
     2881 ccagcgcttc aaccttcgcg agtcgggatg tccctcctct cgcgggccgc gcgcggcgag
     2941 aggtgacgct acggcgatag atttgtctca aaccagtttc agccgccgca gcgatcgccc
     3001 atggcaaatt cggctgtagc ccgcggccat gcgtgagcgt tgtccggcaa acttcatacc
     3061 ggtggcgggt tgagccgggc gaagccttgc tgggtgcggt agggataata ggggtaagcg
     3121 gacatcacgt cgcttgctgt gttcagccgc gcaatctgtc ccgtcgtcag agaccatccg
     3181 acggcgccaa gattgtcgcg caactgtacc tcgttgcgtg cgccgatgat gacagacgat
     3241 actgtcgggc gggtgagcaa ccacgccagc gcgacttgcg gaatgctctt gccggtttcg
     3301 ccggcgacga tctccagcgc atcggtcaca gtgtagagcc gctcgttgct gaccggcggt
     3361 ccgaattgcg cggtggcatg gagacggctg gcttcgggaa gcggctggcc gcgccggatc
     3421 ttacctgtca atcgtcccca gccgagcggg ctccacacca atgcgccaac gccctgatcg
     3481 aggcccagcg gcatcagttc ccattcgtaa tcgcgtccga tcagcgagta atagacttgg
     3541 tgcgccgcgt agcgcggcca accattctgc gcggcgatcc cgagcgattt catcaattgc
     3601 cagccggaga aattggagac cccgacatag ttgaccttgc ccgcgcgcac cagcatgtcg
     3661 agggtgtaga gcacctcgtc gatcggcgtg ccggcatcga aggcgtgaag ctgcagcaga
     3721 tcgatgtggt cggtgccaag ccgcgtgagc gaggcatcga ccgcccgcgt cagacgctgc
     3781 cgggatgagc cggcatcggc gggcccatcg cccatcggca ggctggtctt gctcgagatc
     3841 atgagcctgt cgcgccggcc cttgatggct gcgccgagcc gttcttccga tccgccattc
     3901 gaatagacat ccgcggtgtc gaacagggtg acgccggcat cgagacagat atcgatcagt
     3961 cggcgcgctt cgtcggcgct gctgttgccc caggccgaga acagcggtcc ctcgccgccg
     4021 aacgtaccgg tgccgaagct caatacggga acctggaggc ccgatgcgcc gagattgcga
     4081 tattgcatgt tggctttcct tcttgccggt taggcggttg ccgagaccag cctgcgatcg
     4141 agctgcacgc tccacagcgc gagggcgagg ccaaggagcg tgatcgcagc ggcgaccagc
     4201 ggcagggcgg caaagccaag cccggcatcg atcgtcacgc ccccggccca tgcgccgagc
     4261 gcattgccaa gattgaaggc cgcgatgttg agactggaag cgaggttctg tccggcgctt
     4321 ccggctttct cgagcacgcg aagctgaagc ggcgcgaccg tggcgaaagc cgcaacgccc
     4381 aacaggccga ccagcagcac cgtcggaatc ttcgtcccca gggcaggccc cagcgcgccg
     4441 aggacgatgg cgaggacggc cagcgtgccg atcagggcgc gcgccaggcc gcgatcggcg
     4501 aaccggccgc ccatgacgtt gccaacggcc agcccggcgc cgagcaccag caggaccggg
     4561 gaaacggcgc tctgcgaaaa tccgctgatg cgggtgagga tcggctggat ataggtgaag
     4621 accgtaaaca gcccggcgaa gccgaacacg gtcattgcga ggcccagcag cacctgcggc
     4681 cgcgcgagaa cccccacctc gtcgcggagc gatgtcggtt cgtcgtcgcg accgagatgg
     4741 cctggcacag agaccgcgag tatggcgaag gcgatcacgc cgattgctgc caccgcccag
     4801 aaagccgcgc gccagccgag cagcaagcca agccaggccc cgaacggcac gccgagcaac
     4861 gtggcaactg tgaggccaac gaacatcgtc gaaatcgccg atgcgcgctt gttctgcgcg
     4921 accagtccgg tcgccaccac cgatccgacg ccgaagaacg tgccatgcgc gagcgacgtc
     4981 agcacgcggg cggccatcag ccagccgtag ctcggcgcca gcgcgcaggt ggcattgccg
     5041 agcgtgaaga tggccatcag ggccagcagc accgccttgc ggggcatgcg ccggctcgcc
     5101 agcgtgagca acggggcgcc cacgaagacc ccgagggcat agcccgagat cagcaggccg
     5161 gcggtcgaaa ccccgacatg catgtcggcc gccacctgca gcaagaggcc catgatgatg
     5221 aattcggtgg tgccgatgcc aaaggcaccg acggtaagcg catagacggc aatgggcatg
     5281 gggcgattcc gaaagcgaga gggacgaccg gacctacatg gtgcggggca gcaactagaa
     5341 ttagggcgcg acaattccat tcatttatga gatgaattca tcatgccacg cctcgatacc
     5401 aaccgcgccg ccgagatgga ggtgttcgtt cgcgtcgtcg atctcggcgg attcaccgcg
     5461 gcagcgcggg aattcaggct gacgccatcg ggcgtgagca agctggtatc ccgtctggaa
     5521 agccggctcg acagccgcct ggtcaaccgc tcgacgcgga ggctgcagct cacgccggaa
     5581 ggccaggtct tctatgatcg cgcggtgcgc gtgctcgccg atctggatga agccgagcgc
     5641 gaagcgtcgg ccggcgcctg cccgcgcggc catttgcggg tcaacagcaa cattccgttc
     5701 ggcatgcgcc acatcatgcc gctggtgccg gcgtttctgg cgctgaatcc cgaggttacc
     5761 ctcgacctcg tgttctcgga cattgtcgtc gatctcatgc aggagcgcgc cgacgtcgcc
     5821 attcgcgtcg gccccttgcg cgactctgcc ttgcttgcac gcaagctcgg cgcgagccgg
     5881 atggccgttg tcgctacgcc agaatacctt gcgaaatcgg gcacacccgc caatcccgac
     5941 gatctggcca agcacaaggg cgtgggctgg accttcgccc gcagcatcgg cggctggccg
     6001 ttcttgtccg gcaaggcaac cgttgaggtc tcgccgccgg tggcgcgcgc cagcgatggc
     6061 gaggccgcgc gcgcgctcgc gctgggtggc gtcggcctgg cgcggctcgc gctgtttcac
     6121 atccgccctg acatcgaagc cggccggctg atcccggtgc ttcaggattt caatccgggc
     6181 gactgcgaag atatccacgc cgtcttcctc ggccagggcg gaccgctgcc ggcgcgggtg
     6241 cgcgcgttca tcgattttct gggtgaaaac atccggctcg atgatctcga tcttgaacgc
     6301 acgcgaaacg acgaatggcg ggtcaggtct gctcgagccc gacttcgatg atttccttct
     6361 caacgaagca gtggaccgtc agcacgcgcg gtcagcgggt cctacagtgc aagacccggt
     6421 ctcggccatg cgacggcaat cggacgaccg gtgcctgaca gcgtgatgaa ggctgtgtgt
     6481 accgaaagct taagctcggg cgtggtggtg gtgaagtccg ccaggtcgcg ttgaaagccg
     6541 cgaaaatcat atcccaaagc gaatgtccac ttcgcgccaa aagcagtgtc gccgccaatt
     6601 actgagtaca tgggtcctaa tcggggacgc gcaaccggcg atgccgacgg tcgtttcagc
     6661 ttgcgatcag gcttcatcgc atgcttctcc ctttcagata cgccgcgctg gccgccagct
     6721 caatccatgt tctatattgg atttgaagga gacggctgcg tcggtgggac caaacagtca
     6781 gtcgcccaaa aggtctgtcg aattcaacgg aggcgcagca tgctgaaatc gatcgatcca
     6841 attctaaatg ccgacgtcct ttacgcgttg cgggcgatgg gccacggcga cgaactcgtc
     6901 atctgtgaca cgaatttccc ggccgacgcg accgcccgtc aaaccgtgct gggacggctg
     6961 ctccggatcg ataacgtaac ggctggccgg gcggcgcgcg ctattctttc agttatgccg
     7021 ctcgatacct ttgtcgacaa gcccgctttg cgaatgcaaa tcgtcggcag gcctgacgag
     7081 gttcctcccg tgcagatcga ggtgcagagg gagatcgacg ctgcgcaggg cggccctttg
     7141 tcaatgggct cgatcgagcg atttgccttc tatgaactcg cgaagaaatc ctattgcgtg
     7201 atccagaccg gcgaacggcg cttctacggc tgcttcgtgt tcaagaaggg agtcat
//
LOCUS       DASWGM010000964         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1342701,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000964 DASWGM010000000
VERSION     DASWGM010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342701"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VGA15_25970"
     CDS             <1..467
                     /locus_tag="VGA15_25970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX9591176.1"
                     /translation="RGFGGAGLFIISYAIETVYSILLCPILWFGHTIFLTGLLFGREI
                     GWIGQTRDDHAVPFALALRNLWPHTLLGCAALGLLAVTQPAAIPYALFLAGGPALAIP
                     FAMMTAWPWLGHFAARVGIGRLPEETARPAVLLALALPAIEQAAPLPRPSSV"
     gene            472..1218
                     /locus_tag="VGA15_25975"
     CDS             472..1218
                     /locus_tag="VGA15_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX9591177.1"
                     /translation="MFEGFRTARGIFRSLRIYYGDRSRAVAMDRLYGGFVRRGDRVFD
                     IGAHVGDRVASFRRLGARIVAVEPQPAMVKVLKLLYGRCIDVAIEPVAVGRSVGATSM
                     MINADNPTVSTASREFVSAARDAPGWETQRWIKSVPVPVTTLDALIGKYGAPAFIKID
                     VEGFEEEALQGLSVAVKALSFEFTIIQRHVALACIERCIALGYTRFNAALGESQVFVN
                     ADWVGGEEIARWLTGLPHAANSGDIYAALG"
     gene            1244..2539
                     /locus_tag="VGA15_25980"
     CDS             1244..2539
                     /locus_tag="VGA15_25980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591178.1"
                     /translation="MVLAVTALIAAGYGLTLTIFYPGILTYDAKFVYEDIAKGVLGDW
                     QSPVMTVLWGAIDPVAPGAGSMFLLIATSYWLGFGLLAFALARRSTRLTLLLPLLALM
                     PPAFVFVGIIWRDVLFATTWLLAAAIAFVAAERGARLRVPALALALALCGFGVLLRPN
                     ALIAAPILGAYIVWPTQMSWKRTAILLVPAMAGFFALVQVVYYGALGATRQHPLHSLM
                     VFDLGGISHFSKQNQFPVTWSEAESALLLNTCYQPTQWDIYWRLEPCDFVMRKVEREE
                     KLFGTPAITEAWARAVMHHPLAYLGHRTAFMWNFLWGNNPTMWLADVERPTETVFPDR
                     PAFLALVFLHDMLKLTPLFRTGSWLLVCLIVCGFAWRRRETPEGAFALGVCGSAAVYV
                     LSFFAVGVASDFRYGYWAVLAGMVGGVVAALGRPKAQVP"
     gene            complement(2553..3065)
                     /locus_tag="VGA15_25985"
     CDS             complement(2553..3065)
                     /locus_tag="VGA15_25985"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9591179.1"
                     /translation="MRILAVVGGLAILVGIGAAVFFFGGYYSVAGTAEDPAIVHWALT
                     QVRTASINRYAHDQPPPAINISDPATIQAGAKAFATHGCTNCHGAPGVAWAKFSEGLH
                     PDPPDLKEIVDHRTPAQLFWVVKNGINMTGMPSFALAGVKDDEIWSIVAFLKKLPGVS
                     EADYKAWSAP"
     gene            3297..>3537
                     /locus_tag="VGA15_25990"
     CDS             3297..>3537
                     /locus_tag="VGA15_25990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9591180.1"
                     /translation="MPAHTKRVAVLVPCFNEEAAVATVVADFRKALPSAEIFVYDNNS
                     SDRTMAVAREAGAEVRSERRQGKGHVVRRMFADIDA"
BASE COUNT          555 a         1109 c         1146 g          727 t
ORIGIN      
        1 gccgcgggtt tggcggcgcc ggactgttca tcatcagcta cgccatcgaa accgtgtact
       61 cgatcctgct ctgtccgatc ctgtggttcg gccacacgat cttcctcacc ggacttttgt
      121 tcggccgaga aattggctgg atcggacaga cccgtgacga tcacgcggtg ccattcgcgc
      181 tggcgctgcg caatttgtgg ccgcacacgc tgctcggatg cgcagcactt ggtctgctgg
      241 cggtgacgca gccggcggcc attccctatg cgctgtttct cgccggcggc ccggcgttgg
      301 cgatcccgtt tgccatgatg accgcgtggc cttggctcgg ccattttgcc gcccgggtcg
      361 gcattggccg gctgcccgag gaaaccgcga ggcccgcggt gctattggcc ttggcgctgc
      421 ccgcgattga gcaggcggcg ccgttgccgc ggccgagttc ggtctgaggc ggtgttcgag
      481 ggtttcagga ccgcgcgcgg tatttttcga tcgcttcgca tctactacgg cgacaggtcg
      541 cgcgcagtgg cgatggaccg gctctatggc ggcttcgtgc ggcgcggcga tcgggtgttc
      601 gatatcggcg cccatgtcgg cgaccgcgtg gcctcgttcc gccggctcgg cgcacggatc
      661 gtcgcggtcg agccgcagcc cgccatggtc aaggtgctca agctgctcta cgggcgctgc
      721 atcgatgtcg cgatcgagcc ggtggccgtc ggccgcagcg tcggcgcgac cagcatgatg
      781 atcaatgccg ataacccgac cgtctcgacg gcgtcgcgtg agttcgtaag cgcggcacgc
      841 gatgcgcccg gatgggaaac gcagcgctgg atcaagtcgg tgccggtgcc ggtaacgacg
      901 ctggatgcgc tgatcggcaa gtacggcgca ccggccttca tcaagatcga tgtcgaaggt
      961 tttgaggaag aggcgctgca gggactctcc gtcgcggtca aggcgctgtc gttcgagttc
     1021 acgatcatcc agcgccatgt cgcactcgcc tgcatcgaac gctgcatcgc gctgggttac
     1081 acgcgattca atgcggcgct cggcgagagc caggtttttg tgaacgccga ttgggtcggc
     1141 ggcgaagaga tcgcgcgctg gctgaccggg ctgccgcacg cggccaattc cggggacatc
     1201 tatgccgccc tcggctgacc gcacgcagcc gatgcggcga cgcttggttc tcgctgtcac
     1261 cgcgctgatc gccgccggct acggcctgac gctaacgatc ttttatcccg gcatcctcac
     1321 ctacgacgcg aaattcgtct acgaggacat cgccaagggc gtgctcggcg actggcaatc
     1381 ccctgtcatg accgtgctgt ggggcgcgat cgatccggtt gcgccgggtg cgggcagcat
     1441 gttcctgctg atcgcgacct cttactggct gggtttcggc ctcctggcat tcgcgcttgc
     1501 gcgccgctca acccggctca cgctgttgct gccgctgctg gcgctgatgc caccggcctt
     1561 cgtttttgtc gggattatct ggcgcgacgt gctgttcgcg accacctggc tgctggccgc
     1621 cgcgatcgcc tttgtcgcgg ccgaacgcgg cgcacgcctt cgcgtgccgg cgctagcgtt
     1681 ggcgctggcg ttgtgcggct tcggcgtcct gctgcgtccg aatgcgctga tcgcagcacc
     1741 aatcctcggc gcttacatcg tctggccgac gcagatgtca tggaagcgca cggcgatcct
     1801 gttggtcccc gccatggccg gatttttcgc gctggtgcag gtggtctatt acggcgcgct
     1861 cggggcgacg cggcaacatc cgttgcattc gctcatggtg ttcgacctcg gtggcatcag
     1921 ccacttcagc aaacagaacc agtttccagt cacctggagc gaggctgaga gcgcgctgct
     1981 cttgaacacc tgctaccagc cgacccagtg ggacatctat tggcggctcg agccgtgcga
     2041 tttcgtgatg cgcaaggtcg aacgcgagga aaaactgttc ggcacgccgg cgatcaccga
     2101 agcctgggcg cgtgcggtca tgcaccatcc gttggcctat cttgggcatc gcacggcgtt
     2161 catgtggaat ttcctttggg gtaacaaccc cacgatgtgg ctcgccgatg tcgagcgccc
     2221 gaccgagacc gtatttccgg atcgcccggc cttcctcgcg ctcgtctttc tgcacgacat
     2281 gctcaagctg acgccgctgt tccgcaccgg ctcatggctg ctcgtttgcc tcatcgtgtg
     2341 cggcttcgcc tggcggcgac gggagacgcc cgaaggcgcc ttcgccctcg gcgtttgcgg
     2401 atcggccgcc gtctacgtgc tcagcttctt tgccgtcggc gtcgcgtcgg actttcgtta
     2461 cggctattgg gcggtccttg ccggcatggt cggcggcgtg gtggccgcgc tgggccggcc
     2521 caaggctcaa gtgccgtgaa tgacgctgca ggctatggcg cgctccaggc cttgtaatca
     2581 gcttccgaga cgcccggcag ttttttcagg aacgcgacga tcgaccagat ctcatcgtcc
     2641 ttgacaccgg ccagtgcgaa gctcggcatg cccgtcatgt tgatgccgtt cttgaccacc
     2701 caaaacagct gcgccggcgt gcgatgatcg acgatctcct tcaaatccgg cggatcggga
     2761 tgcagccctt cggagaattt ggcccaggcc acgcccggcg cgccatgaca attggtgcag
     2821 ccatgcgtgg cgaacgcctt ggcgccggcc tgtatggtgg ctgggtcgct gatgttgatg
     2881 gcggggggag gctggtcatg cgcgtagcga ttgatcgacg cggtgcgcac ctgggtcagc
     2941 gcccaatgga caatcgccgg gtcttcggcg gtccccgcga cactgtaata acctccgaaa
     3001 aagaacaccg ctgcgccgat gccgacaagg atggccagcc cgccaaccac tgcaaggatg
     3061 cgcatgtggg tctccaagat gttcgtctgc gttggccgtt agtcctgata tcatataccg
     3121 ggcaacagct tgcatacgta gcgatatcga aacatcggct actaattccc ggagtgaaca
     3181 gaattatttt gcccgaggca agcgcttgct gatttacgtc agtggaacca accgtaaaga
     3241 atctgattgc agtataaaca tccatggaca ggcttgattc ggtaaccggg ccggcgatgc
     3301 cggcacacac gaaacgggtg gccgttctgg tgccgtgctt caacgaggaa gccgcggtcg
     3361 ccaccgtggt cgccgatttt cgtaaagccc tgccatcagc cgaaatcttt gtctatgaca
     3421 acaattcgtc ggatcgcacc atggcggtag cgcgcgaggc cggggcggag gtgcgcagcg
     3481 aacgacgtca gggcaagggc catgtggtgc ggcggatgtt cgccgatatc gatgccg
//
LOCUS       DASWGM010000965         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_238978,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000965 DASWGM010000000
VERSION     DASWGM010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238978"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VGA15_25995"
     CDS             <1..409
                     /locus_tag="VGA15_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9591181.1"
                     /translation="VGLDEPAYGAFLNWMYFSDATLTFPQTLVLRYTELEPEERRNPQ
                     VATDYAKWFLGRLRAVEAATAKAETLCADRFTAADIVIGYALRLADNIGLAKDFGPNV
                     AAYWQRLQARDGYKRAVAAEILAGEQQKVARRS"
     gene            526..1347
                     /locus_tag="VGA15_26000"
     CDS             526..1347
                     /locus_tag="VGA15_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9591182.1"
                     /translation="MNDRDRRDSGRLMLISPERVFYAGLLGRPRKRSSGGFNIYTAMQ
                     GSLKITGDKSEVVGEVALVRPYVPHSVESEHSSIICLVIEPETVEPSAMDGLGARICG
                     AEAPAIALRIRTAYETLRRQERRGGFTTAEFDQLSFGERLPDRNIDRRIRCAALMLND
                     FSGSKMTAADCAASVQLSPSRFLHLFKDQTGISFRAFRAWKRARHLLHFVNEDINLAH
                     LAQDIGYPDSTHFSHSIRRFYGLQPRAIFSGSRDLAIYRNDPGVRDSAGFRQGRP"
     gene            1459..>1632
                     /locus_tag="VGA15_26005"
     CDS             1459..>1632
                     /locus_tag="VGA15_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX9591183.1"
                     /translation="MSDKAVITCALNGVLTDPKQHHVPVTPEEMAREAKAAFNAGASV
                     MHIHLRQQEPDKGH"
BASE COUNT          307 a          520 c          520 g          285 t
ORIGIN      
        1 ggttggcctc gacgagccgg cctatggcgc cttcctgaac tggatgtatt tcagcgatgc
       61 gacgctgacc tttccgcaga cgctggtgct gcgctacacc gagctcgagc ccgaggagcg
      121 gcgcaatccg caagtcgcca ccgactacgc caaatggttc ttggggcggc tgcgcgccgt
      181 cgaggccgcc accgccaagg cggagacgct gtgcgccgat cgcttcaccg cggccgacat
      241 cgtgatcggc tacgcgttgc ggcttgccga caacatcggt ctggcaaaag atttcgggcc
      301 taacgtcgcc gcctactggc agcgcctgca ggcgcgcgac ggctacaagc gggccgtcgc
      361 cgcggaaatt ctcgccggcg agcagcaaaa ggtcgcgcgg cgctcgtagg gcatgatccg
      421 gaaacgtcgg tgacatccgg cgcgatttct gtattctggc aaggctccgc agagagcccc
      481 gccagcgcgg taagaaccag aaatcgctcc cgggagggat ggaccatgaa cgacagggat
      541 cgccgtgatt ccgggcggct gatgctgatc agccccgagc gggtgtttta tgcggggctg
      601 ttgggccggc cgcgaaaacg atccagcggc ggcttcaaca tctacactgc gatgcaggga
      661 agcctcaaga tcaccgggga caagtccgag gtggtcggtg aagtggcact ggttcggcct
      721 tacgtgcccc acagcgtcga aagcgagcat tcctcgatca tttgcctcgt gatcgagccg
      781 gaaacggtcg agccctcggc catggatggc ttgggcgcca gaatttgcgg agcggaggcg
      841 cctgccatcg cgctgcgcat tcgtaccgct tacgagaccc tgcgccggca agagcggcgt
      901 ggcggtttta ccactgcgga attcgaccaa ctgtcgtttg gcgagcgttt gcccgatcgc
      961 aacatcgatc gtcgcatccg atgcgccgct ttgatgctca atgatttctc cggcagcaag
     1021 atgaccgcgg ccgactgcgc cgcatcggtc cagctgtcgc catcgcgctt cctgcatctg
     1081 ttcaaggacc agaccggcat ctcgtttcgg gcatttcgcg cctggaagcg cgcgcggcat
     1141 ctcttacatt tcgtcaacga agacatcaat ctggcgcatc tcgcgcagga catcggctat
     1201 cccgactcga cgcatttcag ccattcgatc cgccgtttct acggcttgca gccgcgcgcg
     1261 attttttctg gctcaaggga cctggcgatc taccgcaacg atcccggcgt gcgcgacagc
     1321 gccggtttcc gtcaggggcg gccatagcgg cggcgcgctg gccccgcgat agcgcgtgga
     1381 cgcaagaagc caagcggtgc gggacgcatc atccgccagc cctgcgggtg aagacctcga
     1441 gcgacaaaag gctgatccat gagcgacaag gctgtcatca cctgcgcgct gaacggcgtg
     1501 ctgaccgacc cgaaacagca ccacgtcccg gtaacaccgg aggaaatggc gcgcgaggcc
     1561 aaagcggcgt ttaacgccgg cgccagcgtg atgcatatcc atctgcgcca gcaggagccg
     1621 gacaagggac at
//
LOCUS       DASWGM010000966         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_799380,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000966 DASWGM010000000
VERSION     DASWGM010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799380"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VGA15_26010"
     CDS             <1..586
                     /locus_tag="VGA15_26010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX9591184.1"
                     /translation="RPYDRDRDGFVMGEGAGIVVLEEYEHAKQRGAKIYAEVIGYGLS
                     GDAYHITSPTPDGDGAFRSMSAAMKRAAITSSDIDYINAHGTSTQVGDEIELGAVERL
                     LGNAASRVSMSSTKSSTGHLLGAAGAIECIFSVLAIRDNIAPPTINLDHPSVETAIDL
                     VPRTPRKREINVALSNSFGFGGTNASVIVQRVVN"
     gene            727..1992
                     /gene="mltG"
                     /locus_tag="VGA15_26015"
     CDS             727..1992
                     /gene="mltG"
                     /locus_tag="VGA15_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX9591185.1"
                     /translation="MSERPPISPRSPRAALEPERVPPPPKRSARARNPFVVFGNAVIT
                     IVLVLMIGAGGVYVYGRQKLEAPGPLQEDKVVNIPARAGMTDIADILQREGVIDNNRW
                     AFIGSVFALKARSELKPGEYSFQKSASLRDVIGTIVEGKVVQHAVTIPEGLTSEQIVA
                     RLSENDVFAGAVREMPREGTLLPETYKFPRGTSRDQVIARMQQTQKRVLAEIWERRNP
                     DIPVKTPEQLVTLASIVEKETGKADERSRVAAVFVNRLRQKIKLQSDPTIIYGLVGGR
                     GTLGRPIKRSEIQQPSPYNTYVVEGLPPGPIANPGRASLEAAANPARTRDLFFVADGT
                     GGHAFTETYDQHQKNVAKLRTMEKQIQNDTVEPADDGQPPPAGTPVDAAAPTATTAKP
                     AAPKKPARNAAPARQGAAQSTAAPPVVQQ"
     gene            2176..3063
                     /locus_tag="VGA15_26020"
     CDS             2176..3063
                     /locus_tag="VGA15_26020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX9591186.1"
                     /translation="MALSSMTGFARSHGASGPYAFEWELKSVNAKGFDLRMRLPPGWD
                     ELESFAKKRAGEMLSRGTVYANLNVKRANAVSTIRINEEVLASIVKVAGVLAGKIDAV
                     APSIDGLLGIKGVIEVVEPEADEAEDKAAQAAAAAAFELALANLVEMRRREGVTLGQI
                     LSQRMDEIEILAKQAEAAPGRKPDAIRARLVEQIAALLESSERFDPDRLHQEAIMVAA
                     KADIREELDRIASHISQAREMIGKGGPVGRRLDFLAQEFNREVNTCCSKSNDLELTNT
                     GLAMKNVVEQFREQVQNLE"
     gene            3067..>3559
                     /gene="gmk"
                     /locus_tag="VGA15_26025"
     CDS             3067..>3559
                     /gene="gmk"
                     /locus_tag="VGA15_26025"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX9591187.1"
                     /translation="MTAGGHGFDGVERRGLMFVLSSPSGAGKTTLSRLLIERMPGLKM
                     SVSATTRPMRPGEVEGHDYLFVDQSKFEAMVKRNELLEWATVFENRYGTPRAPVEAAL
                     SAGQDVLFDIDWQGTQQLREKARADVVSVFILPPSAADLERRLHSRAQDSDQVIRGRM
                     SRAS"
BASE COUNT          715 a         1085 c         1151 g          608 t
ORIGIN      
        1 gcggccctac gaccgggatc gcgacggttt cgtgatgggg gagggggccg gcatcgtggt
       61 gcttgaagaa tacgagcacg ccaagcagcg cggcgcgaag atctatgcgg aggtgatcgg
      121 ttacgggtta tccggcgacg cttatcacat cacgtctccg acgccggatg gcgatggcgc
      181 gttccgcagc atgagcgcgg ccatgaagcg cgccgcaatc acttcttccg atatcgacta
      241 catcaacgcg catggaacgt cgacgcaggt aggagacgag atcgagctcg gtgcggtgga
      301 gcggttattg ggcaacgccg cttccagggt gtcgatgtcg tcaaccaaat cgtcgaccgg
      361 tcatcttttg ggcgcggcgg gagcgatcga gtgtatcttc agcgttcttg cgattcgcga
      421 taatatcgcc cctccaacca tcaatctcga ccacccatcg gtagaaacgg cgatcgatct
      481 cgtgccgcga acgccacgca agcgcgagat caatgtcgcg ctatcgaatt cgttcggttt
      541 cgggggcacc aacgcatccg tgatcgtgca gcgcgtcgtg aactgacgcg ccatatgaat
      601 catataaaga agagtccacc gattcgccgc attcggtgat gaatcctagg gctgggcgta
      661 gactatcggc cgacgtggaa aatttcgcgg ggccgtgatt gaaccgacag gatttaggtc
      721 gcatcgatga gtgagaggcc gcccatttcg ccaaggagtc cgcgcgcagc gctggagccc
      781 gagcgggtcc caccgccgcc gaagcgatcc gcgcgcgccc gcaatccgtt cgtggttttc
      841 ggcaatgcag taattaccat cgtgctggtc ctgatgatcg gcgcgggcgg ggtctatgtc
      901 tacggcaggc agaagctcga agccccgggg ccgttgcagg aggacaaggt cgtcaacatt
      961 ccggcgcgcg cgggaatgac cgacattgcc gatatcctgc agcgcgaagg cgtcatcgac
     1021 aacaatcgct gggcgttcat tggcagcgtg tttgcgctga aggcgcgctc cgaactcaag
     1081 cccggcgaat actcgtttca gaagagcgcc agcctgcgcg atgtgatcgg taccatcgtc
     1141 gaaggcaagg tggtccagca cgccgttacg atacccgaag gcctgacctc cgagcagatc
     1201 gtggcccggc tttccgagaa cgacgttttt gcgggcgccg tccgcgaaat gccgcgcgag
     1261 ggtaccttgc tgccggaaac ctacaagttc ccgcgcggca cctcgcgcga tcaggtgatt
     1321 gcgcgcatgc agcagacgca gaagcgggtg ctggccgaaa tatgggagcg ccgcaatccg
     1381 gacattccgg tcaagacgcc ggagcaactc gtgacgctgg catcgatcgt cgagaaggaa
     1441 accggcaagg cggacgaacg cagccgggtc gctgcggtgt tcgtcaatcg gctgcggcaa
     1501 aaaataaagc tgcagtccga tccgaccatc atctatgggc tggttggcgg caggggaacg
     1561 ctgggacggc cgatcaaacg ctccgaaatt cagcagccgt ctccctataa tacctacgtg
     1621 gtcgaggggc ttccgccagg gccgatcgcc aatcccgggc gcgcctcgct ggaagccgcc
     1681 gccaatccgg cacgcacccg cgatctgttc tttgtcgcgg acggcaccgg cggccacgcc
     1741 tttaccgaga cgtatgacca gcatcagaag aacgtcgcca agctgcgcac catggaaaag
     1801 cagattcaga acgacacggt cgaaccggct gacgatggcc agccgccccc ggcaggtacg
     1861 ccggtcgacg ccgctgcccc cacagccacc accgccaagc cggcggcccc gaaaaagcct
     1921 gcccgcaatg ccgctccggc ccggcaggga gccgcgcagt cgacggcggc gcctcccgtg
     1981 gttcagcaat agtgcaagcc cgccggcatg cgccggagcc tgatggcacc tggcccggaa
     2041 aacgctgcta actcgtaact tgtgcgtatt ctacttgcaa aacccgtatc cacttttgcg
     2101 caatacgcgc taaggtcgca gcagtggttg gctttgcgaa tctcaccccc gttccgttcc
     2161 ggagaatttt acgcgatggc gctgtcgagc atgactggtt ttgcccgaag tcacggcgcc
     2221 agcggaccct acgcgttcga atgggaactg aagtctgtca acgccaaggg ttttgatctg
     2281 cggatgcggc tgccgcccgg gtgggacgaa ctcgaaagct tcgccaagaa gcgcgccggc
     2341 gaaatgctgt cgcggggtac ggtgtacgcc aatttgaacg tcaagcgcgc caacgcggtc
     2401 tctacgatcc gtatcaacga ggaggtgctc gcctcgatcg tcaaggtcgc cggcgtgctc
     2461 gccggtaaga tcgacgcggt agcgccgagc atcgacgggc tgctggggat caagggggtc
     2521 atcgaggttg tcgagccgga agccgacgag gccgaagaca aggctgcgca ggctgccgca
     2581 gcggcagcat tcgaactggc gctcgccaac ctcgtcgaga tgcgccggcg cgagggggtg
     2641 acgctcgggc agatcctctc gcagcgcatg gacgagatcg agatcctggc gaagcaggcc
     2701 gaggccgcac ccggccgcaa gcccgacgcg atcagggcac ggcttgtcga gcagatcgcg
     2761 gcactcctcg aatcgtccga acgcttcgat cccgaccggc tccatcagga ggcgatcatg
     2821 gtcgccgcca aggccgatat tcgcgaagaa ctggaccgga tcgcctcgca tatctcgcag
     2881 gcccgcgaga tgatcggcaa gggcggacca gtcggccggc gtctcgattt cctggcgcag
     2941 gaattcaacc gcgaggtcaa tacctgctgc tccaagtcga acgacctcga actgaccaac
     3001 accggccttg cgatgaagaa tgtggtcgag caattccgcg aacaggttca aaatctggag
     3061 tgaaccatga cggctggcgg ccatggcttc gacggggttg agcggcgcgg attgatgttc
     3121 gtgctgtcgt cgccgtcggg cgcggggaag accacgctgt cgcgcttgct gatcgaacgg
     3181 atgccaggac tgaagatgtc ggtgtccgcg accacgcggc cgatgcggcc cggcgaggtc
     3241 gaaggccacg actacctgtt tgtcgaccaa tccaaattcg aggcgatggt aaagcgaaac
     3301 gagttgctcg agtgggccac cgtgttcgaa aatcgctatg gtacaccgcg cgcgccggtc
     3361 gaagcggcct tgtcggcggg acaggatgtg ctgttcgata tcgattggca gggcacgcag
     3421 caattgcggg aaaaggcgcg ggcggacgtt gtcagcgttt tcatcctgcc gccctccgcc
     3481 gccgacctgg aaagacgcct gcactcccgc gcgcaggatt ccgatcaggt catccggggg
     3541 cggatgagcc gcgccagcc
//
LOCUS       DASWGM010000967         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_398002,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000967 DASWGM010000000
VERSION     DASWGM010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398002"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1515)
                     /gene="argH"
                     /locus_tag="VGA15_26030"
     CDS             complement(169..1515)
                     /gene="argH"
                     /locus_tag="VGA15_26030"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX9591188.1"
                     /translation="MEEINVSIDVDRHLYAQDIAASKAHAAMLSAQGIITATDAKNIA
                     KGLDTILSEIGNGSFEFKRALEDIHMNVESRLSELIGPAAGRLHTARSRNDQVATDFR
                     LYVRDTIDETDAALSAFQHALVQRALEHAGTVMPGFTHLQTAQPVTFGHHLLAYVEMA
                     ARDRGRFADARKRLNESPLGAAALAGTSFPIDRDLTAKTLGFDRPMANSLDAVSDRDF
                     VLETLSAAAIASVHLSRFAEEIVIWTSPLVGLVRLSDKFTSGSSIMPQKRNPDAAELV
                     RAKTGRVIGALTALLIVMKGLPLAYQKDMQEDKQGAMEAFGALSLAIRAMTGMVADVV
                     PDEARMKAAAGEGYATATDLADWLVRTLKMPFRDAHHVTGRIVAEASNAGVPLHELPL
                     KDMQAIEPKITVEALSVLSVEASVKSRTSYGGTAPKNVLAQAKAWLKRLEKERKLG"
BASE COUNT          273 a          488 c          475 g          290 t
ORIGIN      
        1 gccgccaagc gcgagcaccg ccgcgctgag caggatgatg gcccatcgcg aagatgtcgg
       61 acagtcagta cgattcacgg caaaatcccc aatgcggcgg caccatacaa agattgccct
      121 gttctggcga gacccaggcc aacatgaatc gcagcgaatt tcgtgagctc agcccaattt
      181 tcgctctttt tccagtcgtt tgagccacgc cttcgcctgc gcaaggacat tcttcggcgc
      241 ggtcccgcca tagctcgtcc ggcttttcac ggaggcttct accgagagaa cgcttaaggc
      301 ctccaccgtg atcttcggct cgatcgcctg catgtccttc aagggcagtt cgtgcaaggg
      361 cacgcccgcg ttcgatgcct cggcgacgat tcgcccggtg acatgatggg cgtcgcggaa
      421 cggcatcttc agcgtccgca ccagccagtc ggccagatcc gtggcggtgg cgtagccctc
      481 cccggcggcg gctttcatcc gcgcctcgtc cggcacgacg tcggcgacca tgccggtcat
      541 cgcccggatc gccagcgaga gcgcgccgaa cgcctccatc gcgccctgct tgtcctcttg
      601 catgtccttt tgataggcca gcggaaggcc cttcatcacg atcagcaagg cggtaagcgc
      661 gccgatcacc cggccggtct tggcgcgaac cagttcggcg gcgtcggggt tgcgcttctg
      721 cggcatgatc gaagagccgg aggtgaattt gtcggatagt cgcaccagcc cgaccagcgg
      781 cgaggtccag atcacgattt cctcggcgaa acgcgacagg tgcaccgaag cgatcgccgc
      841 cgccgacaag gtttcgagca cgaagtcgcg gtccgacacc gcgtcgagcg aattggccat
      901 cggccggtcg aagccgagcg tcttggcggt caagtcgcga tcgatcggga acgaggtgcc
      961 ggccagcgcc gcggcgccga gcggggattc attcagacgt ttgcgcgcgt cggcaaaacg
     1021 gccgcggtcg cgcgccgcca tctcgacata ggccaggaga tggtgtccga acgtcaccgg
     1081 ctgcgcggtc tgcagatggg tgaaacccgg catcaccgtg ccggcatgtt ccagcgcccg
     1141 ctgcaccagt gcgtgctgaa aggccgaaag cgccgcatcg gtctcgtcga tcgtgtcgcg
     1201 gacgtagagc cggaaatcag ttgccacctg atcgttgcgc gaccgcgcgg tgtgcagccg
     1261 ccccgccgcg ggcccgatca gctcgcttag cctactctcg acattcatgt gaatgtcctc
     1321 gagcgcgcgc ttgaattcga acgatccatt gccgatttct gacaaaatcg tgtctagacc
     1381 tttagcgata tttttcgcat cggttgcggt aatgatgcct tgggctgata gcatcgcggc
     1441 gtgggccttg gacgcggcga tgtcctgggc ataaaggtga cgatcgacgt cgatggagac
     1501 gttgatttcc tccatgatcg catcgg
//
LOCUS       DASWGM010000968         7279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2731449,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000968 DASWGM010000000
VERSION     DASWGM010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2731449"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VGA15_26035"
     CDS             <1..426
                     /locus_tag="VGA15_26035"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX9591189.1"
                     /translation="LPFVASPLGRARSTMELVRGAIKLPPDDYAIDDRLREIGYGQWE
                     GLTLPEMELHDAATFASRNEDKWGVAAPSGESYASVTVRVREWFDSLVADTVAVAHGG
                     TMRALMVALGIASPLEAAETPIGQGVVYIFSDGGMRKYG"
     gene            644..1744
                     /gene="aroC"
                     /locus_tag="VGA15_26040"
     CDS             644..1744
                     /gene="aroC"
                     /locus_tag="VGA15_26040"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX9591190.1"
                     /translation="MSFNTFGHLFRVTTFGESHGVAIGCVVDGCPPLLPLAVEDIQRD
                     LDRRRPGQSRFTTQRQEADAVKILSGVMAHPETGVQVTTGTPIALLIENTDQRSKDYS
                     DIKDKFRPGHADYTYEAKYGLRDYRGGGRSSARETATRVAAGAIARKILPGVTVRGAL
                     VQMGPHKIDRDKWDFDEIAKNPFFCPDKDKAAFFEGYLDDIRKKGSSIGAVIEVIAEG
                     VPAGLGAPIYAKLDAELAAALMSINAVKGVEIGSGFGAAELSGEENADEMRMGNQGVS
                     FLSNHAGGILGGISTGQPVVARFAVKPTSSILSPRRTVDRKGADTDILTKGRHDPCVG
                     IRAVPVGEAMMACVLTDHFLRHRGQVGGEPQR"
     gene            1804..1953
                     /locus_tag="VGA15_26045"
     CDS             1804..1953
                     /locus_tag="VGA15_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591191.1"
                     /translation="MADTVPASEFTQHRRSFATAELSDERVQAIGSSRMDERHAHLDA
                     VLEPN"
     gene            2133..3317
                     /locus_tag="VGA15_26050"
     CDS             2133..3317
                     /locus_tag="VGA15_26050"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9591192.1"
                     /translation="MNIPDLQKITDWLIDGARSAPGPTRMMSETCERLVAAGLPLWRV
                     NVFIRTLHPDIFGFSFVWRPGEEVTVTSASFDAPDSQEFKASPLAILYETASEVRYRL
                     DDPQAAKFPFLDDMRAEGVTDYFAVPLLFADGLVNSSSWTTKQPGGFSNQQLDSLRSL
                     VVPLARLIEIISLRRRASTLLDTYVGNRAGERILGGQIRRGHTDTMHAAIWLSDLRGF
                     TALSDRLPAETVVEILNRYFDCQVSSIRSHGGEVLKFMGDGLLAVFPIDEYVGDIHQV
                     CSRVLEAARESRASVDAMHFQVGEAVERFRFGVALHVGKILYGNIGGGNRLDFTCIGP
                     AVNLAARLEKIAGHLHRTIVASEGFAGICAGGWTELGEFPIAGFSKTERVYGLMDETP
                     GG"
     gene            complement(3341..3646)
                     /gene="clpS"
                     /locus_tag="VGA15_26055"
     CDS             complement(3341..3646)
                     /gene="clpS"
                     /locus_tag="VGA15_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX9591193.1"
                     /translation="MIDSVTKPKTKVKTKTERPRLHKVILVNDDFTPREFVVTVLKAE
                     FRMTEDQAHKVMITAHRRGVCVVAVFTKDVAETKATRATDAGRAKGYPLLFTTEPEE"
     gene            complement(3734..4615)
                     /locus_tag="VGA15_26060"
     CDS             complement(3734..4615)
                     /locus_tag="VGA15_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9591194.1"
                     /translation="MFRIDMVCCVLLLAASGSAMAESPTERGSYLVNTIMACGNCHTP
                     RDADGTTIAEKAFSGGGLTFTTPAFVATAPNITPDIETGIGSWSDAEIKRALVEGLRP
                     DHGHLAGVPLAAIMPSSFYKALLSDDLDAIVAYLRTVRPIRNEVPDPVYKAPVRRDSY
                     PDAEAGFSKAMFTDPVRHGAYLVTIGHCMECHSAWSRGVSDFKNGLGRGGRAFPPRAG
                     SPEGSPASVAANITSDPIAGIGGWTDQEIGRAVTHGIARDGRTLKPPMAYRYYAGLED
                     ADLADIIAYLRTVPPLQ"
     gene            4816..5694
                     /locus_tag="VGA15_26065"
     CDS             4816..5694
                     /locus_tag="VGA15_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEX9591195.1"
                     /translation="MRWCVSIGLVLVAGAMAGGDVAGIAAPSTRNQLTNQLGPQPGLE
                     LAAPKDSTPSVDIELVIAVDVSYSMDMDELAIQREGYAQAIVSKEFLQALKTGPNGKI
                     SITYFEWAASSDQKIIIPWRVIDGPETADAVANEIMKTPIRRASRTSISGAINFAMPL
                     FDENPYRGLRRVIDISGDGPNNNGTPVTGARDAALAKGITINGLPIMVKEPSYSTMDI
                     DNLDFYYEDCVIGGPGSFVVSIKDREKFKEAIRTKLLLEVAGRTPERRIVPVADKEPR
                     VSCMIGEKIWQDRWGR"
     gene            complement(6116..>7279)
                     /locus_tag="VGA15_26070"
     CDS             complement(6116..>7279)
                     /locus_tag="VGA15_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX9591196.1"
                     /translation="VPYTLGGETVEVAPVPGHHPDRRRLLRVERASPERITPFCPHFG
                     TCGGCAIQHWEAERYRAWKRDIVVETLASAKLACAVGPLIDAHGLGRRRITLHARIGT
                     HDVLKVGFAAANSHDIIPVDRCPILDPGLSGALDAGWAVAEPLIATGKPLDIQITSTS
                     NGLDIDVRGSGALPAAMIATLSRVAERHRLARLTRHGELVLMRTPPTIPIGTAQVTLP
                     PGAFLQATVAGEEALAALVSEHCSRAKHIADLFCGVGPFALRLAAKSRISAFDSDAGA
                     VAALQKAATSTSGLKPVTAEARDLFRRPLMPQELRDFDAVVFDPPRQGAQTQVLQLAA
                     SKIAVVVAVSCNVATFARDAKILIDGGYKIEGVTPVDQFRHTPHVELVARFAR"
BASE COUNT         1288 a         2280 c         2398 g         1313 t
ORIGIN      
        1 ctgccctttg tcgcaagccc gcttggccgc gcgcggtcga cgatggaact ggtccgcggc
       61 gcgataaaac tgccgccgga cgattacgcc atcgacgacc ggctgcgcga gatcggctac
      121 ggccagtggg aaggcttgac gttgcccgag atggaactgc acgacgcggc gacctttgcg
      181 tcccgcaatg aggacaaatg gggcgtcgcg gcgccctccg gcgaaagcta tgccagcgtc
      241 acggtgcgcg tgcgggagtg gttcgattcc cttgttgccg acaccgtcgc cgtcgcccat
      301 ggcggcacca tgcgggcgct gatggtcgcg ctcggcatcg caagcccgct cgaggccgcc
      361 gagacgccga tcgggcaggg cgtcgtctac atattcagcg acggcgggat gaggaagtat
      421 ggatgaattc ttttccctct cccgcttgcg ggggagggcc agggtggggg cagccgcaaa
      481 caccggcgcc agccgttccc ccaccccatc cctcccccgc aagcgggaga agggggcgca
      541 agagccgcgc cagcgtggtt aactctggtt atttgaccgc tcggaccgcc cgcgttacga
      601 caagccgtag taaagtgtcg cttgtctatc ggaccatcgc gtcatgtcct tcaacacctt
      661 cggccacctg ttccgggtca cgaccttcgg cgagagccac ggggtcgcga tcggctgcgt
      721 ggtcgatggc tgcccgccgc tgctgccgct cgcagtcgaa gacatccagc gggatctcga
      781 ccgccgccgg cccggacagt cgcgttttac gacccagcgg caggaagccg atgcggtgaa
      841 aattctgtcc ggcgtgatgg cgcatccgga gaccggcgtg caggtgacga ccggcacgcc
      901 gatcgcgttg ttgatcgaga acaccgatca gcgctcgaag gattattccg acatcaagga
      961 caagtttcgc ccgggccacg ccgattacac ctatgaagcc aaatacggcc tgcgcgatta
     1021 tcgcggcggc gggcgttcgt cggcgcgcga aaccgcgacg cgggtcgcgg ccggcgccat
     1081 cgcccgcaag atcctgcccg gcgtgaccgt gcgcggcgcg ctggtgcaga tgggcccgca
     1141 caagatcgat cgcgacaaat gggattttga cgagatcgcc aagaatccgt tcttctgccc
     1201 ggacaaggac aaggccgcgt tcttcgaggg ctacctcgac gacatcagaa agaagggctc
     1261 gtcgatcggc gcggtcatcg aagtcatcgc ggaaggcgtg ccggccggcc tcggcgcacc
     1321 gatatatgcc aaactcgatg ccgagttggc cgcggcgctg atgagcatca acgcggtgaa
     1381 gggcgtcgag atcggctcgg gctttggcgc ggcggaattg tcgggtgagg aaaacgccga
     1441 cgagatgcgg atgggcaatc agggcgtcag cttcctgtcc aaccatgccg gcggcattct
     1501 gggcggcatc tccaccggcc agccggtggt ggcgcgcttc gcggtgaagc cgacctcgtc
     1561 aattttatcg ccgcgacgca cggtggaccg caagggtgcc gacaccgaca tcctgacaaa
     1621 gggccgtcac gacccctgcg tcggcatccg cgcggtgccg gtcggcgagg ccatgatggc
     1681 ctgcgtgctg accgatcatt ttttgaggca tcgcgggcag gtggggggcg agccgcaacg
     1741 atgatgcgga ttgtcgtacg tcgtattttt tcgagtcgag ggacgtcaga aaggaccttg
     1801 gacatggctg acaccgtccc ggcttcggag ttcacccagc atcgccgcag ctttgcgacg
     1861 gctgagctgt ccgacgagag agttcaggca atcggctcct cacgcatgga cgagcgacat
     1921 gcgcatctcg atgccgtgct tgaaccgaat taacccgaac cgggtctatg ccatctgcgg
     1981 caccgtgagc atgaagcaag cgtgggccca cgccctctaa gggggcgaga gatttttgca
     2041 cagctcgggc gcttcgcttc gctcgcaatg acgggtctac ggccattgac tcgccttgac
     2101 gtccggaaaa cttgggccgc aaatgcactg ccatgaacat acccgacctg cagaaaatca
     2161 ccgactggct gatcgatggc gcgaggtccg cgccgggccc gacccggatg atgtcggaaa
     2221 cctgcgagcg gctggtggcg gccggcttgc cgctctggcg cgtcaacgtc ttcattcgga
     2281 cgctgcatcc agacatcttc ggcttcagtt tcgtctggcg gccgggcgag gaggtcacgg
     2341 tgacttcggc atcgttcgac gcgccggatt cgcaggagtt caaggcaagc ccgctggcga
     2401 tcctgtacga aacggcttcg gaagtcagat atcggctgga cgatccgcaa gccgcgaagt
     2461 ttccctttct ggacgacatg cgcgccgaag gcgtcaccga ttatttcgcg gtgcccctgc
     2521 tgtttgctga cggtttggtc aattcctcga gctggaccac gaagcagccg ggcggattta
     2581 gcaaccagca actggacagt ctgcgctcac tggtggttcc gctggcgcgg ctgatcgaga
     2641 tcatcagtct gcgccgcagg gcctcgaccc tgctcgatac ctatgtcggc aatcgcgccg
     2701 gcgagcggat tctcggcggg cagatcaggc gcggccacac cgacacgatg catgccgcga
     2761 tctggctgtc ggatctgcgc ggcttcaccg cgttgtccga ccggctgccg gccgagaccg
     2821 tggtggagat cctcaatcgc tattttgatt gccaggtgtc gtcgatcaga agccatggcg
     2881 gggaagtctt gaagttcatg ggcgacgggc tgctggcggt gtttccgatt gacgagtatg
     2941 tcggcgatat ccatcaggtc tgcagccgcg tgctggaggc ggcgcgcgaa tcccgcgcca
     3001 gcgtggatgc catgcatttc caggttggcg aagccgtcga gcgtttccgc ttcggcgtcg
     3061 ccttgcatgt cggaaaaatc ctgtacggca atatcggcgg cggcaaccgg ctcgacttca
     3121 cctgcatcgg tcccgcggtg aacctcgcag caaggctgga aaaaatcgcc ggccacctcc
     3181 accgcaccat cgtggcctcc gaaggcttcg ccggaatatg cgccggcggc tggaccgaac
     3241 ttggcgagtt tccgatcgcg ggcttttcaa agacggagcg cgtctacggg ttgatggacg
     3301 aaacgccggg cggttgaaaa tggcagccgc cgttgccgcg ctactcctcc ggctcggtgg
     3361 tgaacagcag cggataaccc ttggcgcggc cggcatcggt ggcgcgggtc gccttggtct
     3421 cggcaacgtc cttggtgaag accgcgacca cgcatacacc gcgccggtgc gccgtgatca
     3481 tgaccttgtg ggcctggtcc tcggtcatgc ggaattccgc tttcaggacg gtcacgacaa
     3541 attcgcgggg cgtgaagtcg tcgttgacca ggatgacctt gtgcaagcgc ggccgttcgg
     3601 tcttggtctt gaccttggtt ttcggcttgg tgacgctatc gatcattccg gcggcgcctc
     3661 ctgacgttac gggccgtcac gaccagttta caacgtctcg atctgtcgcg aaacctgcag
     3721 ttccgcgatg gcttcattgc agcggcggca cagtgcgcag ataggcgatg atatcagcca
     3781 gatcggcgtc ctctagtccg gcatagtagc ggtaagccat cggcggcttg agcgtccgcc
     3841 cgtcgcgcgc aattccatgg gtgacggcgc ggccgatttc ctgatcggtc cacccgccga
     3901 tgccagcgat ggggtctgag gtgatgttgg cggcgacgct ggctggcgat ccctccggag
     3961 aacccgcacg cggcgggaaa gccctgccgc cgcggcccag tccatttttg aaatccgaca
     4021 cgccgcgcga ccatgcggaa tgacactcca tgcaatgccc gatggtgacc agataggcgc
     4081 cgtgcctgac cggatcggtg aacatggctt tgctgaaccc ggcctcggcg tcgggatagg
     4141 aatcgcgccg taccggggcc ttgtacaccg gatccggaac ctcgttccgg atcggcctca
     4201 cggtgcggag gtacgcgacg atggcgtcga gatcgtctga cagcaacgcc ttatagaaac
     4261 tggacggcat gatcgcggcc agtggaacgc cggcgagatg gccgtgatcg ggtcgcaatc
     4321 cctccaccag cgcgcgcttg atctcggcat cgctccagct tccgattccg gtttcgatat
     4381 cgggcgtgat gtttggcgca gtagccacga aggccggcgt cgtgaacgtc aggccgccgc
     4441 ccgaaaatgc tttctcggcg atcgtcgtgc cgtcggcgtc gcgcggcgtg tggcaattgc
     4501 cgcaggccat gatggtgttg acgagatagc tgccacgttc cgtgggtgat tcagccattg
     4561 cgctgcctga ggcggccagc aacaggacgc aacacaccat gtcgatccga aacatgacgc
     4621 cccctcgttc aatggacgct gtatcgcatg cgaaaccaat gccggaaagc cttagcgagg
     4681 ctgcggatcg gcgaaacgga aggcgggtga aattaaggcg accgggctaa cttgaatgtg
     4741 aagcgcattc catgttcccg cttttcgaag gctgctttgc gtgtcaccat cgcgtcatac
     4801 gacgtgaggg ccgctatgcg ctggtgtgtc tcgatcgggc tggtgctagt cgcgggggcg
     4861 atggccggag gtgacgtcgc gggtatcgcc gcgccgagca ccaggaatca gctgaccaat
     4921 cagctcggcc cgcaacccgg ccttgaattg gccgccccca aggactctac gccgtcggtc
     4981 gatatcgaac tcgtgatcgc cgtcgatgtc tcctattcga tggacatgga cgagcttgcg
     5041 atccagcgcg agggttatgc ccaggccatt gtctcgaagg aattcctgca ggcgctgaag
     5101 accggtccga acggcaagat ttcgataacc tatttcgaat gggcggcctc gagcgaccag
     5161 aagatcatca ttccctggcg ggtgattgac gggccggaaa cggcggacgc ggtcgccaac
     5221 gagatcatga aaacgccgat ccgaagggcg tcgcgcacct cgatctccgg cgcgatcaat
     5281 ttcgcgatgc cgctgttcga cgagaacccg tatcggggtt tgaggcgcgt catcgatatt
     5341 tccggcgatg gccccaacaa caacggcacg ccggtcaccg gcgcacgcga cgcggcgctg
     5401 gcgaagggca tcaccatcaa cggcctgccg atcatggtga aggaaccgtc ctattcgacg
     5461 atggacatcg ataacctcga tttctactac gaggattgcg tcatcggcgg cccgggctcc
     5521 ttcgtggtct cgatcaagga ccgcgaaaag ttcaaggagg cgatccggac caagctgctg
     5581 ctcgaagtcg cgggccggac gcccgagcgc cggatcgtcc cggtcgccga caaggagccg
     5641 cgcgtctcct gcatgatcgg cgagaagatc tggcaggacc gctgggggcg atagatcgca
     5701 ggaggcgtgc cccggatgct gcgcagcgcg ccgctcttgg gcggcgtggt gcgccgctga
     5761 tccggggccc attgtgcact ccgcatggcg ttgggtcccg gctctgcggt gcatcgtaaa
     5821 agaacgctgc accgcgtccg ggacacgagc cacattgctt gaaacgctct caggatgacg
     5881 gatttgcatt ggagccagga cgaggaatgc gttccccgga tgctgcgcag cgcgccgttc
     5941 ttggcggcgt gatgcgctgc tgatccgggg cccatcgtgc actccgcatg gcattgggtc
     6001 ccggctctgc ggtgcatcgt gaccagacga tgcggagcct gccatcgggc gcgcattcgc
     6061 gcgacccgtt ggcatcgccg ggaggacgct gcaccgcgtc cgggacacga gggcgctatc
     6121 tcgcgaatcg cgccaccagc tcgacatgcg gggtgtggcg gaactggtcg accggcgtca
     6181 cgccctcgat cttgtagccg ccatcgatca ggattttggc gtcgcgggca aacgtcgcga
     6241 cattgcatga caccgcgacc accacggcaa tcttgctggc cgcgagctgg agaacttgcg
     6301 tttgcgcgcc ctggcgcggc ggatcgaaca cgacggcatc aaagtcgcgc agctcctgcg
     6361 gcatcagcgg acgccggaac aggtcgcggg cctcggccgt gaccggcttc agtccggacg
     6421 tcgacgtcgc cgccttctgc agcgccgcca ccgcgccggc atcgctgtcg aacgccgaaa
     6481 tccgcgattt tgccgcaaga cgcagcgcga acggtccgac cccgcagaac aggtcggcga
     6541 tgtgttttgc gcggctgcaa tgctccgata ccagcgccgc cagcgcttcc tcgccggcga
     6601 cggtggcttg caggaacgca cccggcggca gggttacctg cgcggtgccg atcggaatcg
     6661 tcggcggcgt ccgcatcagc accagttcgc cgtggcgcgt cagccgcgcc aggcgatggc
     6721 gttcggcaac gcgcgacagc gttgcgatca ttgccgccgg cagcgccccc gagccgcgca
     6781 catcgatgtc gaggccgttg ctcgtagagg tgatctggat gtcgagcggc tttccggttg
     6841 cgatcagcgg ctcggccacg gcccaccccg catcgagcgc gccgctcaag ccggggtcga
     6901 gaatgggaca gcgatccacc ggaataatgt cgtgcgaatt tgccgcggcg aaccctacct
     6961 tgagcacatc gtgagtgccg atccgggcgt gcagggtaat ccgccggcgc ccgaggccat
     7021 gggcatcgat tagcggaccc actgcacagg cgagttttgc cgatgccagc gtctcgacga
     7081 cgatatcgcg cttccatgcg cggtagcgtt cggcttccca gtgctggatc gcgcatcccc
     7141 cgcaagttcc aaaatgcggg caaaatggcg tgatccgctc cgggctggcg cgctcgaccc
     7201 gcagcagacg ccggcgatcg ggatgatgac ccggtacggg cgcaacctcg acggtctcgc
     7261 cgccgagcgt gtagggcac
//
LOCUS       DASWGM010000969         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1218761,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000969 DASWGM010000000
VERSION     DASWGM010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218761"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VGA15_26075"
     CDS             complement(<1..633)
                     /locus_tag="VGA15_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX9591197.1"
                     /translation="MLFVAVLSVSAVVFAFPIAARAGWPERPIKLIVTFPPGSANDAA
                     ARIFADALGKKWGKPVVVEDKPGAEGTIGVGAFVAGQDDHTLLYTVAGSVTVAPLLID
                     KLPYDVDRDLQPIAATTAIVLTLAVSNDLSVRSIPELIDILRSNPGKYAWTSGPTLPR
                     YVFAAFLKRNGLQMNFVSYRDASQPQADLGEGRIQALVTSLTASSSPVQTG"
     gene            complement(745..1242)
                     /locus_tag="VGA15_26080"
     CDS             complement(745..1242)
                     /locus_tag="VGA15_26080"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9591198.1"
                     /translation="MSPVLSSHRVPAHLARRFHQICLGVTAEILVHEDLTPMLWGVMA
                     AVLEQPGCGQRQLANSMGVDAVNFGQMIDFLEQKGLIRRQIDPNDRRARQLYVTRRGT
                     DLRRRLRPSLLSAQERLLAPLSKAECTALLNMLVRVIEANDSYARPGNGRRKPRRKSN
                     SKPAK"
     gene            complement(1474..2502)
                     /locus_tag="VGA15_26085"
     CDS             complement(1474..2502)
                     /locus_tag="VGA15_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX9591199.1"
                     /translation="MLDLTQDKFDDEPSSHPTKQAAADDRVLLDAYSNAVIGVTEQVG
                     PAVVRVETGSKAPNSRERGGLGSGIVISPDGLVLTNNHVVGSSKQIRLRDHEGIVTDA
                     RVLGVDPDTDLALLRADGARDLRHASLGNSKSLRLGQLVVAIGNPLGFESTVTAGIVS
                     ALGRSIRSVSGRTIEDVIQTDAALNPGNSGGPLVSSAAEVIGINTAIINGAQGICFAV
                     ASNTAQFVLSEIIRHGYVRRAYIGVSGQTAPIPRRHAVVAGVDNKMGALLAQIEPDSP
                     ASLAGLLPGDVVIKLDGVDVNGVDDLIRALDRDRIDRTLAMEVLRMGRLRAIDIHPIE
                     RKPSARQG"
     gene            2753..3673
                     /locus_tag="VGA15_26090"
     CDS             2753..3673
                     /locus_tag="VGA15_26090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9591200.1"
                     /translation="MTNSVWDDRLIKMTAAAALFVAIAQPSFGQDLKYKPITVKTPDG
                     LTISAQEWGNPAGAEILFIHGFSQSYLSWMRQVDSDLAKEFRIVTYDLRGHGNSDKPL
                     DPARYRDSRAWGDEVQTVIDAAGFKHPVLVGWSYAGRVISDYVATHGAGTLVGINFVD
                     AGIKADPALVGDNLKNLPLMASEDLTTNIAATRAFVHGCFSKQPTADDFEIMLSFNMM
                     VPPKIRAGLSGRPLDATEIMSKLKLPVLVTHGAEDRNVKVGVAKYTASVIPGARLSIY
                     EGIGHAPFYEDATRFNTELAAFVREANKTN"
     gene            4046..>4852
                     /locus_tag="VGA15_26095"
     CDS             4046..>4852
                     /locus_tag="VGA15_26095"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEX9591201.1"
                     /translation="MSNKAKKTSNRLTVLFVNSNKLAPGLYGDGNNLFLSVRPSGSRS
                     WVFRFRERGGNRIRDMGLGTAGPDGVPLQAARDRAVALRADLRAWRDPIADKKAAAAA
                     APEAPQKTFGDYAINDWLPVYAMGFKNPRSAKELKRVLEINAAPLFSLALDAIGKPEV
                     LSVLTPIWTTKAKTARELRARLETILQAATPQHRQGENPARWRGNLDSVFGKETKAKR
                     KLRIRKHPAVPYPELPKVVAAIRERHKDADTNVNLALEYLLLTAARTTEVR"
BASE COUNT          966 a         1512 c         1464 g          910 t
ORIGIN      
        1 tccggtctgc accggagagg acgatgccgt cagagacgtc accagggcct ggatccggcc
       61 ttcgccgaga tcggcctgcg gctgcgaggc gtcacgataa ctcacgaagt tcatctgcag
      121 accgtttcgt ttcagaaacg cagcaaagac atatcgcggc aaggtcggtc cggacgtcca
      181 ggcatatttg ccaggatttg atcgcaagat gtcgatcaat tccgggatgc tccggaccga
      241 cagatcgttg ctcacggcaa gcgtcaagac gatcgcggtc gtggcggcga tcggctgcag
      301 atctcgatcg acatcgtagg gcaatttgtc gatcagcaac ggggccactg ttacggaacc
      361 ggcgacggta tagagtagag tatggtcatc ctggccagcg acgaaggcgc cgacaccgat
      421 ggtgccttcc gcgccgggct tatcctcgac cacgaccggc ttgccccatt tcttcccaag
      481 cgcatcggcg aatatccggg ccgcggcatc gttggcgctg ccgggcggga acgtcacgat
      541 cagttttatc gggcgctcgg gccacccggc acgcgcggca attggaaaag caaagaccac
      601 ggccgacacg gagagaacgg cgacgaagag cattcgccgt atggcgcacg cgctcgcctc
      661 ttggcggtga gatatcgcat cgcaaaagcc cgtccacgga aatgagacgg tcgacgctcg
      721 cgagagccac gtcatcgtcc cctcctattt cgccggtttc gaattggact tgcggcgcgg
      781 ctttcgtcgg ccgttaccgg gacgcgcgta ggaatcgttg gcttcgatca cccgcacgag
      841 catgtttagg agggcggtgc actcggcttt cgagagcggc gccagcaggc gctcttgcgc
      901 cgatagcagg gagggccgca gccggcggcg caagtcggtg ccgcgccggg tcacgtaaag
      961 ctgtcgcgcc cgccggtcgt ttggatcaat ctgccgtctg atcaggcctt tctgctccag
     1021 aaaatcgatc atttggccga aattgaccgc atctaccccc atgctgttgg caagttgccg
     1081 ctggccgcat cctggctgct ccagcacggc ggccatcaca ccccaaagca tgggcgtcaa
     1141 atcctcgtgc acgagaattt ccgccgtgac gccaaggcaa atttgatgga agcgacgggc
     1201 cagatgcgcg ggcacccggt gcgaggacag caccggggac atttcagttg gttcagagcg
     1261 cggctcgcgc atggcacgtc caaatagtaa gaattataat gataagaata cgtactatcc
     1321 ggatttgtca atagtgtctg agcgctcggt tcaagtcagg aaacgcttga ccacgcatct
     1381 gaccgacagg acggtcagat gcggccagtg ctcacggcaa acgccgccgt ccaggaaatc
     1441 ccggcggaat cgaagccatc gcacgaggga agctcatccc tgccgggctg acggcttgcg
     1501 ctcgatcggg tgaatgtcga tcgcgcgcag tcggcccatt cgcaacactt ccatcgcaag
     1561 cgtacggtcg atccggtcgc gatccagcgc gcggatcaag tcgtcgacgc cgttgacgtc
     1621 gacgccgtcg agtttgatga cgacgtcgcc gggcaacagc ccggcaagtg atgccggact
     1681 atccggctcg atctgcgcca gcaaggcacc catcttgttg tcaacaccgg cgactaccgc
     1741 atgccgtctt ggaatcggcg cggtctggcc ggacacgccg atataggcgc gccggacgta
     1801 accgtggcgg atgatctcgg acagcacgaa ctgcgcggtg ttgctcgcga cggcaaaaca
     1861 gatgccctgc gcgccgttga tgatcgcggt gttgatgccg atcacctcgg ccgcggacga
     1921 caccagcggg ccgccggagt tgccgggatt gagcgctgca tcggtctgaa tcacgtcctc
     1981 gatggttcgg ccgctcaccg agcggatcga gcgccccagt gccgagacga tgcccgccgt
     2041 gacggtcgac tcgaagccta agggattgcc gatcgcgacc accagctgtc cgaggcgcag
     2101 gctcttcgaa ttgccgagcg acgcgtggcg caggtcccgc gcgccgtcgg cgcgcagcag
     2161 cgcgaggtcg gtgtcgggat cgacgccgag cacgcgcgcg tcggtgacga tgccctcatg
     2221 gtcgcgcagc ctgatctgtt tcgacgatcc caccacatgg ttgttggtga gcaccaggcc
     2281 atccggagag atcacgatac cggagccgag cccgccgcgc tcacggctgt ttggcgcttt
     2341 cgaaccggtt tccacccgca ccacggcggg cccaacctgc tcggtcacgc cgatcacggc
     2401 gttggaatag gcatcgagca gaacccgatc gtcggcggcg gcttgcttgg tcgggtgcga
     2461 tgacggttcg tcatcgaatt tgtcttgcgt aagatccaac atggcgaatt cctccgcctg
     2521 tcaaatggtg acggtgtttt cgcgccgcaa gaaccacctt ccgcacggat ttttgatatc
     2581 ctggttttga tatccaagtc ttgatatcca ggtctgcccg gacgcccggc gtcaccgtcc
     2641 ccagacgtat tttggaatgc gtcagccaat cggcacacgc ccctgcccga ctttcacccc
     2701 cttggcgcat gcgctagctt ggccaataac aaaaaacaaa gggagggttg cgatgacgaa
     2761 ctccgtttgg gatgatcgcc tcatcaagat gacggctgcg gccgctttgt ttgttgcgat
     2821 agcgcagccg agtttcggac aggacttgaa gtacaaacca atcaccgtga agacgcccga
     2881 cggtctaacc atatccgcgc aggaatgggg taacccggcg ggggcggaaa tcctgttcat
     2941 ccacgggttc tcgcagagct atttgtcctg gatgcgccag gtcgacagcg acctcgccaa
     3001 agagtttcgc atcgtgacct acgatctgcg cggccacggc aattccgaca agccgctgga
     3061 tccggcgcgg taccgggatt ccagggcctg gggcgacgag gtgcagacgg tgatcgacgc
     3121 cgccgggttc aagcacccgg ttctggtcgg ctggtcctac gctggccgtg tcatctccga
     3181 ttatgttgcg acccatgggg caggcacatt ggtcggcatc aacttcgtcg atgccgggat
     3241 caaggccgat ccggcgctgg tcggcgacaa tctgaaaaac ctgcccttga tggcgtccga
     3301 agatctcacc accaacatcg ccgcgacgcg tgccttcgtg cacggctgtt tctccaaaca
     3361 accgaccgcc gacgatttcg agatcatgtt gtcgttcaac atgatggtac cgcccaaaat
     3421 tcgcgccggg cttagcggcc gtccgctcga tgcgaccgag atcatgtcca agctgaagct
     3481 gcctgtcctg gttacccacg gcgcggaaga ccgcaatgtc aaggtcggcg tcgccaagta
     3541 caccgcctcg gtcatcccgg gcgccaggct ttcgatctat gagggtatcg gccacgcccc
     3601 gttctatgag gacgcgacac gcttcaacac cgagctggcc gcctttgtgc gggaagccaa
     3661 caagaccaat tgatcgctcc gcaacgattg gcgtgaacga tctttgccat cgcgcgctcc
     3721 aggcttgatc ggaagttctt gcggaagttt ttgccgtccg gctcggatcg gcgtccgtag
     3781 atgcgggtcg gggaagcagg gatggtgggc gcgacaggga tcgaaccaat ttttagccca
     3841 aaaagccacc ctgccgattt gccaaagagc gtcttgtaat cgcagcagtt tttgcgctat
     3901 acctttgaaa tcgtttattt ttatcgcgcc aatcaccttc taatctgagt ccgccaagga
     3961 ccaccacgaa ccgccggaga ccgccatgat ccggcgtttg tgtgtggacg aatgtgtggg
     4021 cgcgacaggg aatctgagtc cacccatgag taacaaagcc aagaagacct ccaaccggct
     4081 gaccgtcctc ttcgtcaatt ccaacaaact tgctcccggc ctctatggcg acgggaacaa
     4141 cctcttcctt tcggtacggc cgtccggcag ccgaagttgg gtattccgat ttcgcgagcg
     4201 cggcggtaac cgtatacgag acatggggct tggcaccgct ggaccggacg gcgtgcctct
     4261 tcaggccgcc cgtgaccggg cggtggcgct gagggccgat cttcgcgcct ggcgtgaccc
     4321 tatcgcagac aagaaggcag ccgccgctgc ggccccggaa gcgccccaga agacgttcgg
     4381 tgactacgct ataaacgatt ggctccccgt ctacgccatg ggcttcaaga acccccggag
     4441 cgcaaaggaa ctgaagcggg tcttggaaat caacgccgcg cccctcttct cgctggcgct
     4501 ggacgccatc ggcaagcccg aagtgctctc ggtgctgact cccatttgga cgaccaaggc
     4561 aaagactgcg cgggagttgc gggcccgcct tgaaaccatt ctacaggcag ccaccccaca
     4621 acataggcag ggcgaaaacc ccgcccgctg gcgcggcaac cttgatagcg ttttcggcaa
     4681 agagacgaag gccaagcgca agctgcgcat tcggaagcac cccgctgtcc cctaccccga
     4741 acttccgaag gttgtggctg caatccgtga gcgccataag gacgccgaca cgaacgtcaa
     4801 tttggccctc gaatacttgc tcctgaccgc cgcccgcacg accgaagttc gc
//
LOCUS       DASWGM010000970         5819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_962500,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000970 DASWGM010000000
VERSION     DASWGM010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962500"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGA15_26100"
     CDS             complement(<1..507)
                     /locus_tag="VGA15_26100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591202.1"
                     /translation="MLAWAALLVAIVVSFRPAVPVIWGDTPSFVESALRTLEAGRPTV
                     AGGRDPGYPAFLAVTFGFGGDLGTVVRLQQAAWAVLMLALAATAQAATRSAGGLVPII
                     LVATYPGLLLFRNVLTAELLFAVFLNLAMAGLLVATCVGKAARCYAVAASVLCAALAA
                     CFRSQGILV"
     gene            complement(680..1615)
                     /locus_tag="VGA15_26105"
     CDS             complement(680..1615)
                     /locus_tag="VGA15_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX9591203.1"
                     /translation="MSGDVMAFDIEAHRGGRALFPENTLQSFANALSMGVDTLELDIG
                     VTRDGAIVVSHERGLNPDLARAAGVYVAPPGIPFVQLRLEEVKKYDVGQIRPGSAYAA
                     QFPEQRAVPGTPIPTLKEVFDLVRRSGNSHVRLNIETKIDPNHPEESPDPQRFVTLLL
                     DLLQAEKFSDRVMVQSFDWRTLQLVQKLAPAIPTVYLSLQKGSAATVFLDKTSDWTAG
                     FDPIKHGGSLPRAIKAAGGAIWSPYFGDVDPALISESHRLGLLVVVWTVNKPADIARM
                     IDIGVDGIISDHPELLRRVAGEKGIALPIGSLVTP"
     gene            1768..2544
                     /locus_tag="VGA15_26110"
     CDS             1768..2544
                     /locus_tag="VGA15_26110"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX9591204.1"
                     /translation="MARKLIDISVPLQNDVPADPPGIHPVIQYNDHQQSLPRMLGFFE
                     GLKAEDLPDGQGWAMETVQLSTHNGTHLDAPYHYHPTMNRGERSWTIDEVPLEWCLQP
                     AVKLDFRHLPDGYVATAKDVEGELKRIGHTLSPLEIVVVNTSAGAKYGQQDYVNSGCG
                     MGYEATMYLLERGVRLTGIDGWSWDAPFVYTAKKFAETRDASLIWEGHKAGRHIGYCH
                     IEKLHNLELLPSTGFTVSCFPVKIERASAGWTRAVAILDG"
     gene            2711..4021
                     /locus_tag="VGA15_26115"
     CDS             2711..4021
                     /locus_tag="VGA15_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591205.1"
                     /translation="MDLFSRLRRSVALLSAVTATSLVLAAPASAQETFKLGIVTFLSG
                     PAADSFGVPARNGAQYVIDQLNMGSAPSPYEKVGFGGMKIEPVIIDENGGATKQVQEL
                     RNLYQRDNVDAVIGYIGSGDCLAVAPIADELKKLLLLFDCGTPRIFEEAKYNYVFRTA
                     AHATMDNVALIRYMKSHNVKMDTFSAINQDYAWGQDSRADFVAAAGQLYPEAKLGSDL
                     LPKFGAGQYGTEISALVGAGSDVVYSSLWGGDLQAFILQSAPRGLPKRSQLVLSAADH
                     VLPPLGDKMPDGTIIGARGAYGLMSQKSPINDWLFKGYEAANSVYPVQAAYRVTQSIL
                     GLKSAVEKAMAKNGGKKPSTDELVEAMTGLEWQSPGGLIQMKLADGHQAIQPIAISRT
                     KYNPDSKRVELVDIQNFPAECVNPPVGVKAIDWIKGGMAGAKCD"
     gene            4181..5077
                     /locus_tag="VGA15_26120"
     CDS             4181..5077
                     /locus_tag="VGA15_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591206.1"
                     /translation="MNFFLTIILDGLIQASWLFIVALGLTLVFGVLKILNIAHGSFYA
                     LGAYIAATAVTLVAAHGLPPSVGFVAMLVSVALIASAVGLLLERGLLKMFYGRDEVVL
                     LLVTYAVFLILEDVTKLIWGVNPIYATQPYELFGTLEFAGLNYVGYDLVLIPISAAIG
                     LAVWFGLNRTITGKIVLAVIHNEEMSVSMGVRVNRVYAVAFAFGVLLASLAGALTAPK
                     ISMQPGLSSDVIILSFAIVVIGGLGSVEGAAIGAILVGLARAASVHLMPQAELFMIYL
                     IMAAVLMFRPEGLFKRETARRI"
     gene            5077..>5819
                     /locus_tag="VGA15_26125"
     CDS             5077..>5819
                     /locus_tag="VGA15_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591207.1"
                     /translation="MKPDLMKPSLHPLVMIALVAAVTLFGRVIPTWTLSLATIAASNA
                     LIALGIVVLARTGNVSFGQGLFFAAGGYGVALIANAWGITDAVAQVIIGAVCGGLLGL
                     VIGPLIARYGGIFFGMLTLALSMVFYGALVKSTALGGSDGFNVGRPSLFGMVFTDPRE
                     ADFMLYAVAVITTGLAGIAATILFRSELGLASLAVRENNLRVEYLGISANRVVTVNFV
                     IAAIFAGASGAFALMAQRHIDPQFAYWTTS"
BASE COUNT         1104 a         1839 c         1789 g         1087 t
ORIGIN      
        1 gaccaggatt ccctgcgatc tgaagcacgc cgcgagcgcc gcgcacagga cggaggcagc
       61 gaccgcgtag caccgcgccg ctttgccgac gcaggtggca acgagcaatc cagccattgc
      121 cagattgagg aagaccgcaa agagaagctc cgcggtgagg acgttcctga acagcagcag
      181 gcccggatac gtcgccacca ggatgatcgg caccagccca ccggcgctgc gcgtcgccgc
      241 ctgggcggtt gctgcaagcg ccagcatcag gacggcccac gccgcctgct gcaggcgcac
      301 caccgtcccg aggtcgcctc cgaacccgaa cgtcacggcc aggaacgccg ggtagcccgg
      361 atcgcgccca cccgccacgg tcggccttcc ggcctcgagc gtgcgaagcg cggattccac
      421 gaaggacggc gtgtcgcccc agatgaccgg gaccgccggc ctgaacgaca cgacgatcgc
      481 caccagcagc gcggcccacg ccagcacggc ccatggcgac gggaacccga cgccgcccag
      541 ggaggcggct ccgcccgttg tacgcgctgg agcttcgcgg tcgcaccgca cgatggccgc
      601 ccacacggca agttcgaatg ccgccccagc ttcatgctag accaaaacgc tctacaagac
      661 catcggaggg caccgagtgc taaggcgtaa cgagcgatcc gatgggcaaa gcgatgccct
      721 tctcaccggc aactctgcgc aacagctcgg gatgatccga gataatgccg tccaccccga
      781 tgtcgatcat gcgcgcgatg tctgcgggct tgttcaccgt ccagaccacc accaaaagtc
      841 cgagacgatg ggattcggat atcagcgcag gatctacgtc gccgaagtac ggtgaccaga
      901 tcgcaccccc cgctgccttg atcgcgcggg ggagcgaccc gccgtgtttg atgggatcga
      961 aacctgccgt ccaatccgaa gttttgtcca aaaacactgt cgcagccgac cccttctgca
     1021 ggctgagata caccgtcggt atcgcgggag ccaatttttg gacgagctgc aacgtcctcc
     1081 agtcaaagga ttgaaccatc acacggtcac tgaatttctc tgcctggaga agatcgagca
     1141 gaagcgtgac gaaacgttgg ggatccgggg actcctcggg atggttgggg tcgatttttg
     1201 tttcgatatt gaggcgcacg tggctgttgc ctgatctgcg caccaggtcg aacacttcct
     1261 tcagtgttgg aatcggcgtt cccggcaccg cgcgctgttc cgggaattgc gccgcatagg
     1321 cgctgcccgg acgaatttga ccaacgtcgt atttcttgac ctcttcgagc cgcaattgga
     1381 caaagggaat gcccggcgga gcgacataga cgccggcggc gcgggcgagg tccgggttaa
     1441 gtccacgctc atgggagacg acgatcgcgc cgtcccgggt taccccaata tccagctcca
     1501 gcgtgtccac acccatcgag agcgcgttgg caaacgattg cagggtattt tcgggaaaca
     1561 gcgcgcgtcc gccccgatgg gcctcaatgt cgaaggccat aacgtccccg ctcatgaaaa
     1621 aacaacaggc agcaacaact gtcacgaaag aatggaccac tgccatgacc gctcctctaa
     1681 cctggaatcg tatgttatca gaacagcgtc gaacgggtgc aacggttcca cgaccgccga
     1741 aatgacgaaa ataacggagg aagcgccatg gcgcgaaaac tgatcgacat ttccgtgccg
     1801 ctgcaaaacg acgtgccggc cgatccgcca ggcatccatc ccgtcattca gtacaacgat
     1861 caccagcaga gcctgccgcg gatgctgggc ttctttgaag gcctcaaggc cgaagatctg
     1921 ccggacggcc agggctgggc gatggaaacg gtccagctgt cgacccataa cggcacccat
     1981 ctcgatgcgc cctaccatta tcatccgacc atgaatcgcg gtgagcgatc atggaccatc
     2041 gacgaggtgc cgctggagtg gtgcctgcag cccgcggtga agctggactt tcgtcatcta
     2101 ccggacggct atgtggcgac tgccaaggat gtcgaaggcg agctcaagcg cattggccac
     2161 acgctttcgc cgcttgaaat cgtcgtggtc aataccagcg ccggggccaa atacggccag
     2221 caggattatg tcaattccgg ctgcggcatg ggctacgaag cgacgatgta tctgctcgag
     2281 cgcggcgtgc ggctgaccgg cattgacggc tggagctggg atgcgccgtt cgtctatacc
     2341 gcgaaaaaat tcgccgaaac cagggatgcc agcctgatct gggagggcca caaggctgga
     2401 cgccacatcg gctactgcca tatcgaaaag ctgcacaatc tggagctttt gccgtcgacc
     2461 ggtttcaccg tgtcgtgctt cccggtcaag atcgagcgag cctcggcggg ctggacccgg
     2521 gcggtcgcca ttctcgatgg ctaaatgccg tctcgacggc tagatgccgc atctgcaaga
     2581 cggcttcgct cccttggcat cggtagacgc gccgggaaag cccacctaaa ctcgcgccaa
     2641 caaccgccga cggagcaacg gctcggacaa agcgggcgga cccaccgccg gccatccagg
     2701 ggagaaaatc atggaccttt tttcgcggtt gcgccgctct gtcgcgcttt tatctgccgt
     2761 caccgcaacg tcgcttgtcc tggcggcacc ggcgtccgcg caggaaacgt tcaagctcgg
     2821 tatcgtgacg ttcctgtccg ggcccgcggc ggacagtttc ggcgtcccgg cccgcaacgg
     2881 cgcccaatat gtcatcgacc agttgaacat gggctcggcc ccctcgcctt acgagaaggt
     2941 cggcttcggc ggcatgaaaa tcgagcctgt tatcatcgac gagaacggcg gcgccaccaa
     3001 gcaggtccag gaactgcgta acctctatca gcgcgacaac gtcgatgcgg tgatcggcta
     3061 catcggctcg ggcgactgcc tcgcggttgc accgatcgcg gacgaactga agaagctgct
     3121 attgctgttc gactgcggca cgccgcgcat cttcgaggaa gcgaaataca actacgtgtt
     3181 ccgcaccgcc gcccacgcga cgatggataa cgtcgcgctc attcgctaca tgaaatcgca
     3241 caatgtgaag atggacacat tctcggcgat caaccaggac tacgcctggg ggcaggacag
     3301 ccgggccgat ttcgtcgcgg ccgcggggca gctttatccc gaagccaagc tgggatcgga
     3361 tttgctgccg aagttcgggg cgggtcagta cggcaccgag atctcggcgc tggtcggcgc
     3421 cggatcggac gtggtctatt cgagcctgtg gggtggcgac ctccaggcct tcattctgca
     3481 atcagcgccg cgcgggctgc ccaagcgcag ccagctcgtg ctcagcgccg ccgaccacgt
     3541 gttgccgccg ctcggcgaca agatgccaga cggcaccatc attggcgcgc gcggcgccta
     3601 tggcctgatg tcgcagaagt cgccgatcaa cgactggcta ttcaaggggt acgaggccgc
     3661 caacagcgtc tatccggtgc aggccgccta tcgtgtcacg cagtcgatcc tcggactgaa
     3721 gagcgcggtc gagaaggcca tggccaagaa cggcggcaag aaaccctcga ccgacgaact
     3781 ggtcgaagcc atgaccggcc tggaatggca atcgccgggt ggcctgatcc agatgaagct
     3841 tgcggatggc catcaggcga tccagccgat cgcgatcagc cgcaccaaat acaatccgga
     3901 ttcgaaacgg gtcgaactgg tcgatatcca gaacttcccg gccgaatgcg tcaatccgcc
     3961 ggttggcgtc aaggcgatcg actggatcaa gggcgggatg gccggcgcca agtgcgactg
     4021 aacggcgtcc gcgtctgaag caaatttgaa gcggcgtgag accgacgctc gcgccgtggc
     4081 ctcctgcccg ttcggcgatt tccaatccgc gcagttgctt cctcaaacag catgttgaat
     4141 tctcgacccc gctcgccgtg acctgcatga aacccgcgcc atgaacttct ttctgacgat
     4201 tattctcgat gggctgattc aggcctcctg gcttttcatc gttgcattgg ggctgacgct
     4261 ggtattcggc gtgctgaaga ttctcaacat tgcccacggc agcttctacg cgctcggcgc
     4321 ctatatcgcg gcgaccgcgg tgacccttgt cgcggcgcac ggcctgccgc ccagcgtcgg
     4381 gtttgtcgcg atgctggttt cggtcgcttt aatcgcgtcc gcagtcgggc tcctgttgga
     4441 acgtggtctg ttgaagatgt tctacggccg cgacgaggtg gtgctgctgc tggtgacgta
     4501 tgcggtgttt ctcattctcg aggacgtcac caagctgatc tggggcgtga acccgatcta
     4561 tgcgacgcag ccctacgagt tgttcggcac cctcgaattc gccggcctca attacgtcgg
     4621 ctacgatctg gtgctgatac cgatttcggc cgccatcggc ttggcagtgt ggttcggcct
     4681 caaccgcacc atcacgggca agatcgtgct ggccgtgatc cacaacgagg aaatgagcgt
     4741 cagcatgggg gtgcgggtaa accgggtcta cgcggtcgcc tttgccttcg gcgtgctgct
     4801 cgccagcctt gccggcgcgc tgaccgcgcc gaaaatctcg atgcagccgg gtctcagttc
     4861 ggatgtcatc atcttgagct ttgccatcgt ggtgatcggt ggattgggca gcgtcgaggg
     4921 cgcggcgatc ggcgcgatcc tggtcgggct cgcgcgcgca gcctcggtgc acctgatgcc
     4981 gcaggccgag ctgttcatga tctacctgat tatggcggcg gtgctgatgt ttcgccccga
     5041 gggcctgttc aagcgcgaga cggcaaggcg aatctgatga aaccggatct tatgaagcca
     5101 agtctgcatc ctctcgtcat gatcgcgctg gtggcggcgg tgacgctgtt cggtcgcgta
     5161 attccaacct ggacgctgtc gcttgcgacc atcgccgcat ccaatgccct gatcgcgctc
     5221 ggcatcgtgg tgttggcgcg cactggcaac gtatcgttcg gccagggcct gttctttgcg
     5281 gccggcggtt atggcgtagc gctgatcgcc aacgcctggg gtatcaccga cgcggtggcg
     5341 caggtgatca tcggcgccgt ctgcggcggc ctgctcggcc ttgtgattgg tccgctgatc
     5401 gcccgctacg gcggcatctt cttcggcatg ctgacgcttg cgctgtcgat ggtgttttat
     5461 ggcgcgctgg tcaaatccac cgcgctcggc ggctccgatg gtttcaacgt cgggcggccg
     5521 agcctgttcg gcatggtctt caccgatccg cgcgaggccg acttcatgct gtacgcggtc
     5581 gcggtgatca ccactggcct cgccggcatc gccgcgacga tcctgttccg gtccgaactc
     5641 ggcctcgcca gcctcgcagt tcgtgaaaac aatttgcgcg tcgaatatct gggtatctcg
     5701 gccaaccgcg tcgtcaccgt caacttcgtg atcgcagcga ttttcgccgg cgccagcggc
     5761 gcgtttgccc tgatggcgca gcgtcatatc gatccgcaat tcgcctactg gaccacctc
//
LOCUS       DASWGM010000971         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2731809,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000971 DASWGM010000000
VERSION     DASWGM010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2731809"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="VGA15_26130"
     CDS             <1..1467
                     /locus_tag="VGA15_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-(1-6) glucans synthase"
                     /protein_id="HEX9591208.1"
                     /translation="NSQTPLLPTTQIAPEQIAQDLAQLAKISECVRTYSLENGLDQVP
                     ALAQKVGLKVILGIWLGSNRLKNLAQISTAVGLTKAYPDVITAVVVGNEVLLRGEMTT
                     SDLAANIRSVKSQVAVPVTYADVWEYWLRNREVYEAVDFVTIHILPYWEDFPIRAKHA
                     AAHVDAIRRRMAVAFPGKEILIGETGWPSEGRMREGALPSRTNQARVVSEILGLARQE
                     NFRVNLIEAYDQPWKRQLEGTVGGYWGLIDSVQRAVKYPPGDAISNYPFWKLQMGCGM
                     GLSFLVFGAAWLTLRRRPWTPRVASWIAVGISATTAGILLGVAADKMFYESYGFGGWL
                     GWGALLTAAIASPMLCAHAAMSGRSLPTFLELLGPRDERARSALTMILGLALVVTTLI
                     GSETALGFAFDPRYRDFPFAALTMAVVPFSTLMLLNRPQEGVRPIAEAVFAGLLAGAA
                     LYTVFNEGSDNWQSLWTCAVYFLFAVTLWRARAVQIPK"
BASE COUNT          271 a          459 c          496 g          299 t
ORIGIN      
        1 aacagccaga cgccgctgtt gccgaccacg cagatcgccc cggaacagat tgctcaggat
       61 ctggcgcaac ttgccaaaat atccgaatgc gtgcgcacct attcgttgga aaacggcctc
      121 gatcaggttc ctgcactggc ccaaaaagtc gggctgaaag tcatcctcgg gatatggctc
      181 ggcagtaacc gcctgaagaa cctcgcgcag atttcgaccg cggtcggcct gaccaaggca
      241 taccccgacg tcatcaccgc cgtcgtggtc ggcaacgaag tgctgttgcg cggcgagatg
      301 acgacgtccg acctcgccgc caacatccgc tcggtcaaat cgcaagtcgc ggttcccgtc
      361 acctatgccg atgtctggga atattggctt cgcaaccggg aagtctacga agcggtggat
      421 tttgtcacga tccatatcct tccctactgg gaagactttc cgatccgcgc caaacatgcc
      481 gccgcccatg tcgatgccat tcgcaggcgg atggcggtgg cgtttccggg caaggaaatc
      541 ctgatcggcg agaccggctg gccgagcgag gggcggatgc gcgagggggc actgccgtcg
      601 cggaccaatc aggcgcgggt ggtctcggag attttggggt tggcgaggca ggagaacttt
      661 cgcgtcaatc tgatcgaggc atacgaccag ccctggaaac gccagctcga aggcacggtc
      721 ggcggatatt ggggcctgat cgattcggtg cagcgtgcgg tgaaatatcc gcccggcgac
      781 gccatcagca actatccgtt ctggaagctg cagatgggat gcggcatggg cttgagcttt
      841 ttggtgttcg gcgcggcctg gctgaccctg cgccggcggc cgtggacgcc gcgcgtcgca
      901 tcgtggatcg cggtcggaat atcggcgacc acggccggaa ttctgctcgg cgtcgcggcc
      961 gacaagatgt tctatgaaag ctacggtttc ggcggctggc ttggatgggg cgcgttgctg
     1021 acggccgcga tcgcttcgcc catgctttgc gcccacgctg cgatgtcagg ccggagcttg
     1081 ccgacctttc tggaactgct gggcccgcgc gatgagcgag cccgatcggc gctgacgatg
     1141 attttgggct tggcccttgt cgtgaccacg ttgatcggca gcgaaaccgc gctcggcttt
     1201 gcattcgatc cacgctaccg cgattttccc tttgccgcgc tgaccatggc cgtggtgccg
     1261 ttttcgaccc tgatgctgct caaccgcccg caggaggggg tgcggccgat cgccgaggct
     1321 gtattcgccg gcctgctggc gggggcggcg ctctacacgg tgttcaacga aggctcggac
     1381 aactggcaat cgctgtggac ctgcgcggtg tattttctgt tcgcggttac gctgtggcgg
     1441 gcgcgggccg tgcaaatccc aaaataagca ggccgagcgc taaacccgac aggccgatat
     1501 tgtagagcac gatcccgaat ccggc
//
LOCUS       DASWGM010000972         7476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2209465,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000972 DASWGM010000000
VERSION     DASWGM010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2209465"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="VGA15_26135"
     CDS             complement(<1..112)
                     /locus_tag="VGA15_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9591209.1"
                     /translation="MPGPLNGVRVLDLTGVVSGPFATMFLADQGADVLKIE"
     gene            complement(215..853)
                     /locus_tag="VGA15_26140"
     CDS             complement(215..853)
                     /locus_tag="VGA15_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX9591210.1"
                     /translation="MDTPLKVEFQFDFGSPNAYLAEFALAGIERRTGAKFEYVPVLLG
                     GIYKATNNMSPAESLRGIRNKPEYQALETQRFIRRHNVTKFRQNPFFPVNTLMLMRGA
                     VAAQFEGVFEPYFRAAYHHMWEEPKKMDDLEVFRSAFISSGIDIEKLIARAQQDDVKK
                     RLIELTNDAVSRGAFGSPTFFVGREMFFGKDQLRDVEESIVEQTSHGFARTA"
     gene            1113..1628
                     /locus_tag="VGA15_26145"
     CDS             1113..1628
                     /locus_tag="VGA15_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9591211.1"
                     /translation="MKWDALEEEPCSLARTIGVIGDRWSLLILRECFLRTRRVEGFQS
                     ALGITRHLLAERLKKLVRQGVLRRIPYQESPKRHEYLLTQKGLDLYPVMMALVHWGDT
                     HMVDERGRPLLHEHRNCGKTFDPVMVCSECGEPLLAKQVHTHPGPGARQASAKLPPDK
                     PAKPRTRKQAA"
     gene            complement(1646..2848)
                     /locus_tag="VGA15_26150"
     CDS             complement(1646..2848)
                     /locus_tag="VGA15_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591212.1"
                     /translation="MFGALKIACALAIALGSSAAFAADNPGVTPTEVKVGGIFPFSGP
                     ASSIGLVGRGILAYVQSINDRGGINGRKINYVAYDDAYSPPKAVEHARKLVESDEVAF
                     MFSQLGTPGNSATAKYLTSKGVPAIAIVSGSNKFTNVTDFPLTTTSLVSFDTEGKIYA
                     KFLTRTLPNAKYAILYQNDDLGKDYVNAFKALLKSDFDKRVVAAAYEITDPTVDSQVV
                     NLKSSGAEALLVAGTPKFAAQAIRKASEIGWKATILLNYPSSSVGATLKPAGLDKSTG
                     VIVGTITMDPTDSQWDNNEGMKNFRAFVDKYMPGVDIADSNYLFGYTQGMLLEQIIKQ
                     CGNDLSRENIIKQAKNLRDVVLPTVLPGIKINTSDKINMDFTQMRLQRWTGTRWDQFS
                     DVLDAASE"
     gene            complement(3391..3837)
                     /locus_tag="VGA15_26155"
     CDS             complement(3391..3837)
                     /locus_tag="VGA15_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9591213.1"
                     /translation="MLDALGDRWAVLILRDLSLGLSRYEDLRRSTGVTHATLSDRLKH
                     LEEKQLIERRRYQTGPERHEYLLTRKGRDTILVMQALAQVGDKWAVSGDAGPPLKFVN
                     RKNGRTLNLALVEEKSGELVGAKDVQPQAGPGADDLVRWRLTKFGN"
     gene            4086..4805
                     /locus_tag="VGA15_26160"
     CDS             4086..4805
                     /locus_tag="VGA15_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591214.1"
                     /translation="MSELRDLVLDAHGGIARWRKLKTIEGDMSITGALWARRGWSDVL
                     RDVHVTADVWGQMVSYQPFTDPGMRSVYRPDYAAVETSDGKLIRDRRNPRAAFDGHSV
                     ETKWDDLDLAYFSGYAIWNYLNSPFLFLLPGVETEEIEPWDEHGEQRRRLKVTFPDSI
                     ATHCSEQVFHIGSDGLISRMDYSAAVTGGVPTAHYSSDYRDFDGIKIATRRRAYRRNP
                     DGTAHTNFIAVAIDIADIRLS"
     gene            4828..5325
                     /locus_tag="VGA15_26165"
     CDS             4828..5325
                     /locus_tag="VGA15_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9591215.1"
                     /translation="MADIDEKRHVVIIEINGVRRTVSVEARKLLVHLIRDDLGLTGTH
                     IGCDTSQCGACTVDIDGQAVKSCTVLAVMADGASITTIEGLSPSGAALHPIQAAFHEH
                     HALQCGFCTPGMIMSVRQLLKNHPNPTEAEIRHDLAGNICRCTGYHNIVRAVEALASE
                     AHSHD"
     gene            5318..>7476
                     /locus_tag="VGA15_26170"
     CDS             5318..>7476
                     /locus_tag="VGA15_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9591216.1"
                     /translation="MTDARAIRYVGQSLRRREDFKLITGKGRYVDDIKLPGMLHMAVL
                     RSPHAHAVINRVDLTEAAAAPGVRLVLSGGDLVGRIGPIVPNWIIPGTKVPVRPVVAV
                     DRVRFVGECVALVVAESQTMAYDAVGLIDVDYETLPAVVDEEAATRDGAPQLHDNVPN
                     NITTVFKVRGGDYQQAAREADQIIRLRVVNNRLIPTCMETRAVIADPNADGTLTVYIP
                     SQVPHMHRRWIAETVGIPEHLLRIVAPDIGGGFGAKMHLYPEDLLCPYLARELGAPVK
                     WWESRSESHQSTSHGRAHTETIEAAIRNDGTILGMRVETSGNVGAYLSNMATGGPTVN
                     TINYGTGTYRIANYEAVSRVIVTNTVPVDAYRGYGRPEGAYIAERTIDAVARHLNLDQ
                     VDVRRKNFIQRADFPYRPYGSMGVMFDSGNYQGCLDKALEAFDYAGRRGECETLRRSG
                     RYRGIGMAAYTHMCGMAPSRRLATSGFDRGGWESARVSIDSSGRATIYSGSMSQGHGH
                     VTSLSQIAADVLQIPIENIDVVQGDTRQVQAGHGTFNSRSMAVGGSSVHVSSGRIVMK
                     AKLIAAGMLEVDEKDVSYSAGEFSVPGTDIRPVTFGKVARMAHVGHKLPDGLEPGLDE
                     TVFYDPTGMGSPSGIHLAYVEVDPETGIVDILDYVAVDDVGTIINPLLAAGQIHGGVV
                     QGIAQALYEEVSYDPDNGQLLTGSLLDYAVPRAEHVP"
BASE COUNT         1525 a         2251 c         2197 g         1503 t
ORIGIN      
        1 gctcgatctt gagcacgtcg gcgccctggt ccgccagaaa catggtcgcg aacggacccg
       61 agaccacgcc cgtcaaatcg agcacgcgaa cgccgttgag tggaccgggc atgacattct
      121 ccttgagtta cgttccgcag cggcgacgat aatccattct ggtctttcaa gccgcggata
      181 tccacctgat gctgatgggc cgcgggccgc gatctcaggc cgtcctggca aaaccgtggc
      241 tggtctgttc gacaatcgat tcttcgacgt cgcggagctg atccttgccg aagaacattt
      301 ccctgccgac gaagaacgtt ggcgagccaa atgctccgcg actgaccgcg tcgttcgtca
      361 gttcgatcag cctcttcttg acgtcgtcct gctgcgcgcg cgcaatcagc ttttcgatgt
      421 cgatgccgga tgagataaac gcgctgcgga agacctcgag gtcatccatc ttcttcggct
      481 cctcccacat atgatggtag gcggcgcgga aatacggctc gaacacgcct tcgaactggg
      541 ctgcgaccgc acctctcatg agcatcagcg tattgacggg aaaaaacgga ttctggcgga
      601 atttcgtgac gttgtggcgg cgaataaaac gctgggtctc cagcgcctga tattccggct
      661 tgttcctgat tccgcgaagc gactcggccg gcgacatatt gttggtcgcc ttgtagatcc
      721 cgccgagcag gaccggaaca tattcgaact tcgcgccagt gcgccgttcg ataccggcaa
      781 gggcaaactc cgccagatag gcattcgggc tgccgaaatc gaactgaaat tcaactttca
      841 ggggggtatc caccggcctc tcctcagggc acgcgtcatt gtgtcagggc tggtcgcgcg
      901 acggccagca gccgtgcctt ggctataaat tcctcatata gttctaaaaa cgaactgtca
      961 ataaaagcct gcttcgggta gtttattgca aatacaaagg ccgctcgggt gattgtctcg
     1021 aattgagtgc ctatttagaa tggcgggacg gccgtggatc gcccagccga ttcgagcgga
     1081 tatcgacgtg ccgtgctgat tggagagcag gaatgaagtg ggatgccctg gaggaagagc
     1141 catgttcatt ggcccgaacc ataggtgtga tcggcgatcg ctggagcctt ctgatcttgc
     1201 gggaatgctt cctgcgaacc cggcgggttg agggatttca gtccgcgctc ggaatcacac
     1261 gccatctgct cgcggaacgg ctgaagaagt tggtccggca aggcgtcttg cgccgcattc
     1321 cgtaccagga gtcacccaaa cgccatgaat accttctcac ccaaaaaggc ctcgatctgt
     1381 atccggtcat gatggcactg gtccattggg gcgacactca catggtcgac gaacgcggcc
     1441 ggccgctgct gcatgagcac cgcaactgcg gcaagacgtt cgatcccgtg atggtatgct
     1501 ccgaatgcgg cgaaccgctt ttggccaagc aggttcatac ccatccgggt cccggcgcgc
     1561 ggcaagcctc ggcaaagctg ccgcctgaca agcctgcgaa accgaggacg cgcaaacaag
     1621 cggcttgaac tggaatcccg cgcgattatt cggacgcggc gtcgagaacg tcgctgaact
     1681 gatcccagcg cgtgccggtc cagcgctgca gccgcatctg ggtaaagtcc atattgatct
     1741 tgtcggacgt gttgatcttg atgcccggga gcactgtcgg aagcacaaca tccctgaggt
     1801 tcttggcctg cttgatgata ttttcgcgcg acagatcgtt accgcactgc ttgatgatct
     1861 gctcgagcag catgccctgg gtgtaaccga acaggtaatt tgaatcggcg atgtcgacgc
     1921 cgggcatgta cttgtcgacg aaggcacgga aattcttcat cccttcatta ttgtcccact
     1981 gcgagtcggt cggatccatc gtgatggttc cgacaatgac gccggtggat ttatcaaggc
     2041 cggcgggctt taaggtcgca ccaaccgaac ttgacgggta gttcaggagg atggtcgcct
     2101 tccagccgat ttcggaggct ttccgtatcg cttgcgcagc gaatttcggc gtcccggcca
     2161 ccagcagcgc ttcggcgccg gagcttttca gattgacgac ctgcgaatcc acggtcggat
     2221 cggtgatttc ataggcggcg gccaccaccc tcttgtcaaa gtcgcttttc agaagcgcct
     2281 tgaaggcatt gacgtagtcc ttgccgagat cgtcgttctg ataaaggatc gcgtacttcg
     2341 cgttcgggag cgtcctggtc agaaacttgg cgtagatttt tccttcggta tcgaaactga
     2401 ccagactggt cgtggtcagc ggaaaatccg tgacattggt gaacttgttc gatccggaga
     2461 cgatggcgat agcgggtacg cctttcgagg tcaggtactt tgccgtcgcc gaatttcccg
     2521 gcgtacctag ctggctgaac atgaaagcca cttcgtcgct ttcgaccagc ttgcgggcat
     2581 gttctaccgc cttcggcggg ctataggcgt catcgtaagc gacgtagttt attttgcgcc
     2641 cgttaatccc gccgcggtcg ttgatggact gaacgtaggc gaggattccc cggccgacga
     2701 ggccgatgga cgaggccggc ccgctgaaag gaaatatgcc accgaccttg acctcggtcg
     2761 gcgtgacccc ggggttatcg gcggcaaagg ctgcggacga tcccagcgcg atagccaacg
     2821 cgcacgctat cttcaatgcc ccaaacattg cgctcccctc cgatttgccg gcgtccgctg
     2881 ccaaggggcg cggtgcatcg cttttatggg cggcggcagc aacgaaaact cacaaccaga
     2941 gaaccggagt tgaactcgtc gtcatccatc gcccacagca atctacagtt ctttttttga
     3001 actagtcaag actttgggtg agcgattgtc acagcaagcc cacgatcgtc accgtttgaa
     3061 cgtcaaccgc aacacaatcc aggacgcgat cgcgctgatt gccgatgtaa ttttccacca
     3121 cgatatgggc atttaccgga aattggggca tgggtgtctg ttcgctcaga tcccttatga
     3181 tcgcaagggc cggcctttgc tgccgatgcg aggtttcaat ctatcgttga tttcatagtt
     3241 cttttttaag actatctgtg gttccggcgc ccgcggaggc gcgcaaagcg aggctctgca
     3301 aagcgataca gagaatggac aaggcatcta ccggattgct cggtcgcgtc gctgttcgga
     3361 ataacgcgac gcagcaacgc ccaaacgaca tcagttaccg aattttgtca ggcgccaccg
     3421 gaccagatcg tccgcaccgg gacctgcctg cggctggacg tccttcgcgc caaccagctc
     3481 acccgacttc tcctcgacca gcgcaagatt cagagtccgg ccgttctttc gattgacgaa
     3541 cttgagcggc ggccccgcgt cgccggacac cgcccacttg tccccgacct gagcgagtgc
     3601 ctgcatcaca aggatggtgt ccctgccctt tcgggtcagc agatattcat gacgctcggg
     3661 acctgtttga tatcgccgcc gctcgatcag ttgcttttct tcgaggtgct tgagccgatc
     3721 ggacagggtg gcgtgagtga cgccggttga ccggcgtaga tcctcgtacc tgctgagccc
     3781 gagcgacaga tcgcgcagga tcagcacggc ccagcggtca cccagcgcgt ccagcacgcc
     3841 ggcgatcgag cacgccattc catcaaaact cttggatcgc atcgacgtca gcctccaggt
     3901 aactcctatt accagagtaa tccaacatcc ggcgccgcca aagctcccgg gaccgcataa
     3961 aaaacgcttg ccttccggta actcttatca ttagagttac agtatcgtcc agcaccggtg
     4021 attgccggaa cgggctggcc gagaaccctg agacccacga actcagccgt gttgaggcaa
     4081 cagatatgag cgaacttcgc gaccttgtcc tcgatgccca cgggggtatc gcacgatgga
     4141 gaaagctgaa gacgattgag ggcgacatgt cgatcaccgg cgcgttgtgg gcaaggaggg
     4201 gctggagcga tgtgctcagg gatgtccacg tcacggctga cgtttggggc cagatggtca
     4261 gctatcaacc cttcacggac cccggcatgc gcagcgtcta tcggcccgac tatgccgccg
     4321 ttgaaacgtc ggatggaaaa ctcatcaggg acagacgcaa cccgcgcgcc gcattcgatg
     4381 gccatagcgt ggaaaccaaa tgggacgatc tggatctcgc ctatttcagc ggctacgcga
     4441 tctggaatta cctcaattcg ccgtttctct ttctgctgcc cggagtagag acggaagaga
     4501 tcgaaccgtg ggatgagcat ggcgaacaac ggcgacggct caaggttacc ttcccggatt
     4561 ccatcgcaac gcactgctcc gaacaggtct ttcacatcgg cagcgacggc ttgatcagca
     4621 ggatggatta cagcgccgcg gtgaccggag gtgttccgac cgcccactac agctccgact
     4681 accgggattt cgacggaatc aagatcgcga ccaggcggcg cgcctatcga agaaatccgg
     4741 acggcaccgc gcatacgaac ttcattgccg ttgcaattga tatcgccgac atccgcctct
     4801 cgtgaggcgc ggggatggga gaaacaaatg gcggacatcg atgaaaagcg ccacgtcgtc
     4861 atcatcgaga tcaatggggt gcgcaggacc gtctcggtcg aagcgcgaaa attattggtt
     4921 catctgatca gagacgatct cggtctgacc ggaacacaca tcgggtgcga tacctctcag
     4981 tgcggcgcgt gcaccgtcga tatcgatggc caggccgtaa aatcctgcac cgtcctcgcc
     5041 gtgatggccg atggcgcgtc gatcacaacc atcgagggtc tgtcgccgtc aggagccgcg
     5101 ctgcatccca ttcaggccgc cttccatgaa caccacgcct tgcaatgcgg cttctgcacg
     5161 cccgggatga tcatgagcgt ccgtcagttg ctcaagaacc atccgaaccc gaccgaagcg
     5221 gaaatccgcc acgacctcgc cggcaacatc tgccgctgca ccggctacca caacattgtc
     5281 cgggccgttg aagcccttgc atcggaggcc cacagccatg actgacgccc gtgcaatccg
     5341 ttacgtcggc caatccttgc ggagacgcga ggacttcaaa ctcatcaccg gcaaaggccg
     5401 ctacgtcgac gacatcaagc tgccgggcat gttgcacatg gcggtgttga gatcgcccca
     5461 tgcccatgcg gtcatcaacc gcgtcgatct cactgaggcc gcggctgcac cgggcgttcg
     5521 gctggtgctt tccggtgggg atctcgtggg aaggatcggc ccgatcgttc cgaactggat
     5581 cattccgggc acgaaagtac ccgtccgccc cgttgtcgcc gtcgaccgcg ttcgctttgt
     5641 cggcgaatgc gtggcgctgg tcgtggccga gtcgcagacc atggcctacg acgccgttgg
     5701 tctgatcgat gtcgactacg agacgctgcc ggcggtcgtc gacgaagagg ctgcgacccg
     5761 cgacggcgcg ccgcagctgc acgacaacgt gcccaacaac atcacgaccg tgttcaaggt
     5821 ccggggtggc gattatcagc aggccgcgcg ggaggccgac cagattatcc ggctgcgcgt
     5881 cgtcaataac aggctgatcc cgacctgcat ggaaacgcgc gccgtcattg cggatccgaa
     5941 cgccgacggc acgctgaccg tctacatccc gagccaggtt ccgcatatgc accggcgctg
     6001 gatcgccgag accgtcggca ttcccgaaca tctgctgcga attgtcgcgc cggatatcgg
     6061 cggcggcttt ggcgcaaaga tgcacctcta ccccgaggat ttgctttgcc cctatctggc
     6121 gcgcgaactc ggcgctcccg tcaagtggtg ggagtcgcgc tccgaaagcc atcaatcgac
     6181 gagccacggc cgcgctcata ccgaaaccat cgaggccgct atccgcaacg atggcacgat
     6241 cctcggcatg agggtcgaaa catcaggcaa tgtcggcgcg tacctatcga acatggccac
     6301 cggcggcccg acggtaaaca ccatcaacta cggcaccggc acctacagaa ttgcgaacta
     6361 cgaggccgtc tcgcgcgtca tcgtcaccaa taccgtgccg gtggacgcct atcgcggtta
     6421 tggacgcccc gaaggcgcct acattgcaga acgcaccatc gatgcggttg cgcgccatct
     6481 caacctcgac caggtcgacg tgcggcggaa gaatttcatt caacgcgccg attttccgta
     6541 tcggccctac ggcagcatgg gcgtgatgtt cgacagcggc aattatcaag gctgcctgga
     6601 caaagccctg gaggccttcg actacgccgg ccgcagaggc gaatgcgaga cgctgcgccg
     6661 aagcggccgt tatcgcggca tcggtatggc ggcctatacc catatgtgcg gcatggcgcc
     6721 gtcgcggcga ttggcgacga gcggcttcga ccgtggcggc tgggaaagcg cgcgggtgag
     6781 catcgattcc agcggccggg cgacgattta ctcgggctcg atgagtcagg gacacggtca
     6841 tgtgacgtcg ctttcgcaga tcgccgccga tgtccttcag atcccgattg agaatatcga
     6901 cgtcgtgcaa ggcgacacga ggcaggttca ggccggccac ggcacgttca attccaggtc
     6961 gatggccgtc ggcggatcga gcgttcatgt gtcgtccggc cgcattgtca tgaaggcaaa
     7021 gctgatcgcc gccggcatgc ttgaagtgga tgaaaaggat gtttcctatt cggcgggcga
     7081 gttcagtgtt cccgggaccg atattcgacc tgtgaccttt ggcaaggtgg cccgcatggc
     7141 ccatgtgggc cacaagcttc ccgacggatt ggagcccggc ctcgacgaga cggtgtttta
     7201 cgatccgacc ggcatgggct cgccttcggg aattcacctg gcctatgtcg aagtcgatcc
     7261 ggagaccggg attgtcgaca tcctcgacta tgttgcggtg gacgacgtcg gcaccatcat
     7321 caatcccttg ctcgccgcgg gccagattca tggcggcgtg gtgcagggaa tagcccaagc
     7381 cctttatgaa gaggtcagct acgaccccga caacggacaa ttgctgaccg gctcgctgct
     7441 cgactatgcg gtgccgcgcg ccgagcacgt ccccaa
//
LOCUS       DASWGM010000973        20876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_800039,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000973 DASWGM010000000
VERSION     DASWGM010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20876
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800039"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGA15_26175"
     CDS             complement(<1..200)
                     /locus_tag="VGA15_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HEX9591217.1"
                     /translation="MTEPPAPYRPNPKLKRVENGFESLIFNSRWLMAPFYLGLVISLA
                     VLLLKFCMVLWEFIVHAPGSKES"
     gene            complement(231..1265)
                     /locus_tag="VGA15_26180"
     CDS             complement(231..1265)
                     /locus_tag="VGA15_26180"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX9591218.1"
                     /translation="MGYKVALVGATGNVGREMLNILDERKFPADEVVALASRRSMGVE
                     VSYGDRTLKVKALEHYDFSDVDICLMSAGGAVSKEWSPKIGAAGAVVIDNSSAWRMDP
                     DVPLIVPEVNADAVAGFVKKNIIANPNCSTAQLVVALKPLHDHARIKRVVVSTYQSVS
                     GAGKDAMDELFSQTKAVYTNSELINKKFPKRIAFNVIPEIDVFMEDGYTKEEWKMMME
                     TKKILDPKIKLTATCVRVPVFVGHSEAVTIEFENPISPDEARDILRNAPGCLVIDKHE
                     PGGYVTPYEAAGEDATYISRIRADPTVDNGLVLWCVSDNLRKGAALNAVQIAECLINR
                     KLISAKKKAA"
     gene            1380..2186
                     /locus_tag="VGA15_26185"
     CDS             1380..2186
                     /locus_tag="VGA15_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591219.1"
                     /translation="MQSFLGRCATLYNPTQIHPVLNSLADECCGMLARLVAYLGALAL
                     LAIVGIHLWDELPATEAVGRSARADWSVATRSHPAFAVSQFDLAEKTETYEILRHPEG
                     GRKDILSWAAPDGKQSLKTVAELEIYRPGDESSGSGPLADIAARIDPEGLHELEAAGI
                     IDSKFGAVTLLRFAGQPGNARSCLGFIKRLEEPNLRISGWTCQGDSPPARRTAIGCIL
                     SRLILLTAGNDPKLAELFAHAELKRGSCTASATSADWVTGAENPRLRGTL"
     gene            2354..2740
                     /locus_tag="VGA15_26190"
     CDS             2354..2740
                     /locus_tag="VGA15_26190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591220.1"
                     /translation="MTWKFPAANKLAVLLAALSAIAVGFAVTAANAEPKRQFDERGRA
                     YYGPNGPNVSYQQGPRTRIYVSRRSWLDAGVEVLPGDRKFTDYAFPTPFGYPSFAREN
                     NNRPIDRQPLNPPSDLGGEPTKFPLY"
     gene            complement(2901..4013)
                     /gene="leuB"
                     /locus_tag="VGA15_26195"
     CDS             complement(2901..4013)
                     /gene="leuB"
                     /locus_tag="VGA15_26195"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX9591221.1"
                     /translation="MATHKLLLLPGDGIGPEVMAEVKRLIDWLNAQGIAHFETEQGLV
                     GGSAYDADKVAISDATMALAQASDAVIFGAVGGPKWDSVPYEVRPEAGLLRLRKDMAL
                     FANLRPAICYPALAEASSLKREVVEGLDIMIVRELTGGVYFGEPKTITDLGNGQKRAI
                     DTQVYDTYEIERIARVAFDLARKRRNKLTSMEKRNVMKSGVLWNEVVTQVHAREYKDV
                     QLEHQLADSGGMNLVKWPKQFDVIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGEIDV
                     KTKKRKSLFEPVHGSAPDIAGRGLANPIAMIASFAMALRYSFEMGALADKVDSAIAAV
                     LANGLRTADIKSEGTTAVSTIEMGEAILKELQALHG"
     gene            4299..5501
                     /locus_tag="VGA15_26200"
     CDS             4299..5501
                     /locus_tag="VGA15_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX9591222.1"
                     /translation="MHAPNPTTSYAHPARLILILSLAPTVCLGIGRFAYSLVLPDMRD
                     ALGWSYSAAGFMNTINAAGYLAGALVASKLIKRLGLSAAVRGGTLACLVSLALCALSG
                     NFIVLSFARLLAGVGAAAAFVGGAALAATIAQSQGSRANFLLSLFYAGPGLGILSSGL
                     IAPFVLQAFGPGSWWIVWWAMTLLSLLMSVPLLLAPIDTKAGIADAAAPTRFAIGPVM
                     IYLAGYFLFGAGYIAYMTFMIAYVRDAGGGAAAQSAFWCLIGLSAFATPWVWRGVLAL
                     DRGGVSTTIILAVNAVGASLPLFGHSMAWLAASALVFGVSFFAIVGSTTAFVRFNYPP
                     SAWPTAIAAMTIAFGVGQILGPIATGAITDALGSLSYALNAGAAMLALGAIATAFQRR
                     LARKSKSA"
     gene            5650..6588
                     /locus_tag="VGA15_26205"
     CDS             5650..6588
                     /locus_tag="VGA15_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9591223.1"
                     /translation="MMTLRQVEVIRAVMVTGTIGGAAKLLNVSAPGISRLVKYTEKSL
                     GIRFFQRQNGRYFPTPEAQSIFEQINGVYKKVDDLTEIISKIGRGALSELRIGSVPSI
                     SQIMVPRAIERVRRRYPDLGIDINILKIEEAIDYLMLGKGDCVAMSYRLAHSGLDFMP
                     LASGELFCIVPQGHELSGRKQVSAAEMIRYPLIGIDPNDPYGRIMAEIFARNKLAYDI
                     TIRARFGTTVCALVKAGLGIAIIDQFTVAHGGYPGVDLLRITEPTRFDTYIAVKRGAS
                     LSLHVEHFIECLRSEMRAIEPSRNAPGAPVSRIRKK"
     gene            6817..8688
                     /locus_tag="VGA15_26210"
     CDS             6817..8688
                     /locus_tag="VGA15_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX9591224.1"
                     /translation="MAKRSIATVSLSGALDEKLRAIAAAGFDAVEIFENDFLSFGGSP
                     RDVGQLCRDLGLQVCAFQPFRDFEGMPEPQRTRNFARAERKFDVMQELQTDLLLICSN
                     VSPASLGGIDRAAADFRELGELAARRGLRVGFEALAWGLHVNDYRDAWEIVRRADHDA
                     IGVILDSFHALAPSFPITAIQSIPADKIFLVQLADAPKLGLDILSWSRHFRCFPGQGD
                     LPVLGFMEAVIATGYAGPLSLEIFNDQFRAGSAARTATDGLRSLILLQDQLAANSAKG
                     SPDALEPKAHSRGVGFLEFAVNETKAGDLADLFGQLGFRKTGKHRSKAVERWSQGKVE
                     LVINCEADGFAHSHYITHGPGVCAIALDVDNADHIMARAEALKARTFYQPVGPGELEI
                     PAIHGVGGSLLYFLDTAGKNWDTDFEAIRNSVTADRLRAVDHISQSMPYDEMLSWLLF
                     YTGILDLQRLPQMEIADPLGLVQSQAIVNTDQSLRIVLNGSVATRTLSARFISEFFGS
                     GVQHIAFSCDDIFLSVAEMRARGASFLEIPDNYYDDVEARYDLDAATMADLRANRILY
                     DREGDGEFFQIYTHIFDERFFFEIVERRTYQGFGAANAPIRLAAQAREVRPLNIPRF"
     gene            8856..10427
                     /locus_tag="VGA15_26215"
     CDS             8856..10427
                     /locus_tag="VGA15_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX9591225.1"
                     /translation="MRPRNSVALLGLLLVGLEGAHGETAGTAVRIDTGEVVGKFQLNA
                     AVRAFLGVPFAAPPVGALRWKPPQPAAPWPNARAATSYSAQCMQPTRSKTSVYYEYAG
                     GDQPTSEDCLYLNVWAPSEKADGKLPVMVWIYGGGFQQGSAANPVFDGARLAARGVMV
                     VSINYRVGIFGFMAHPDLTAESPQHASGNYGLLDQVAGLQWVKRNAAAFGGDPDNVTI
                     FGQSAGAAAVTYLFTSPLARGLFARGICESFGFANKTMTSLADAEKGGAALAEKVAAG
                     SLGDLRQIPSVKLLETHVAMSPIIDGWFLPANPYTMFAQGKEAPVPLLAGWNADEGAT
                     FPHAKTLAAYQEWIRQKYPDIADRLLELYPARTDDEAKTANKRIVRDSLFAWGPWSAA
                     RLHAKNGFATYLYHFNHPQPLRAGVTYDEIDSVDGLGTFHSSEYPYLFGTLDVLTRDW
                     TNTDRMLSDRMQSYWVAYAYSGSPNASGLARWPSFNETAETTMYLGDKTGPGPVPNLD
                     RLELFDTLPSPFGGF"
     gene            10698..11579
                     /locus_tag="VGA15_26220"
     CDS             10698..11579
                     /locus_tag="VGA15_26220"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX9591226.1"
                     /translation="MTKHQPSTRRSFLTGLLGAPIAQSAAPAMHERAAEALGFRCHYQ
                     LIEVAGAGREELKVLLEGVRRLGFAGINVTFPYKEAVVDLLDALSPGAALIGAVNTVI
                     VRDGRLTGHSTDTTGFARAIAPLVAASSRGPVAVIGAGGVGKAIAFALAGLGVGELRI
                     FDSEHAKAMQLAARLEGHVRTKVTHNVEDALRGVAGLVNGSPVGMLPSRDTPVPDALL
                     HAGLWVADAVYSPLWTPLLTAAKAKGATVMTGRELAIYQAADAFELFTGLAPSIAEMG
                     IAFDDVMEKRYAAPHAA"
     gene            11632..12786
                     /locus_tag="VGA15_26225"
     CDS             11632..12786
                     /locus_tag="VGA15_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591227.1"
                     /translation="MKPGILKQGILASALLATVLTGAAFAQSGAPIKLANVAELSGGG
                     ATVGTNWKNGIDLAIEEINAKGGVLGRKLEVTHADSQSNPGVARAQVQKALDSEPYVL
                     LGPGYSGSVKVTAPLAAEAGIAQIMGGEAAELTRGDNKFLFRTSFGQQSSMPKVAKYI
                     NDDIKAKSVAIVWVNNDFGKGGRDVITGEFARYGIKVAADLSTEAGQADFAADVSKIK
                     AAAPDAVFIYLNEEESARILKELKRQSVTAPLIGETTLVGQKVVELAGDAANGARGHV
                     GLTTDAPVELVKAFREKFVKKYNYVPDHNGIKGYLAIYMIKATTEKMGKVDAKAFADN
                     LHGLTIKAANEPGILMDVTFDQKGDIDRQGFLVEIVEGKQVIKQVLPKLN"
     gene            12857..13732
                     /locus_tag="VGA15_26230"
     CDS             12857..13732
                     /locus_tag="VGA15_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591228.1"
                     /translation="MSNLLDLLLAGLATGAIYAIVAVGFTLLWQTSQTINFAQGEFVM
                     LPAFLVLAAMHAGAPFWLAILIGILLSLLLLGLGFKMLLVDPMLRHGVLPLAIATMAL
                     AIGIKEAVKQFFSAEASPFPSVVPAGDIQVFGRLVSLQSIGVLALAILAVVGLTAFLN
                     RTSIGHQMQAAAQNPTVARIIGVPVERMILLTFLINAFLVALASLLITPIYLAKFSSG
                     EVLGQAAFIAAIVGGFNQVRGAIAGGLLIGIVDNLSAAYVSTHYRAAVPLILLIVIIL
                     FRPQGILGRAEERTI"
     gene            13729..14727
                     /locus_tag="VGA15_26235"
     CDS             13729..14727
                     /locus_tag="VGA15_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591229.1"
                     /translation="MTGAGRYWRIALGAIVIAGLIVVPMNFNRYGLFILSQWAVMTIA
                     AMGLNLTLGYAGQVSLAQGAFVGIGAYAAAIMTSQGMPLISALGIAVVVCFAVGWILG
                     YPALRVQHHYLAFVTLAFSTLAFLVFRNEDWLTKGIYGISNIPRPHIFGVATNRPLPF
                     YYVCLGSLGIVSVMLWWLIRSPWGRAFQALRENPIRALSLGIDTRRYTLMAFAIGSAL
                     GGVAGTLYAPLTQYVDPVPFNLSLSLDLLMMVIVGGAGFFFGPFLGAMIAVLLPEWLR
                     FTQGYYLMLYAIAVILLLIYSPTGILGILDRFLAERRTKTAFALRAVAESRLGTAP"
     gene            14724..15467
                     /locus_tag="VGA15_26240"
     CDS             14724..15467
                     /locus_tag="VGA15_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591230.1"
                     /translation="MTAVLEVTNISKRFGGISAVDGVSFDVHEGEILGMIGPNGCGKS
                     TLFNCILGQLTPSDGEVKVDGKVVTGFRPSDLNRLGVSRTFQLLQVFPKLSVRENLIL
                     AGQEHQGSMMSRLFGPSDAGLSEAADRMIGFFKLDHLADEAAGGLSYGQQKLLDAAMA
                     FMGGPRLVLLDEPAGGVNLTMLVDLRERLAAINREKRATFVVIEHNMEFVMSLCSRVM
                     VMAEGRVLAMGKPEEVRANPAVIEAYLGH"
     gene            15490..16233
                     /locus_tag="VGA15_26245"
     CDS             15490..16233
                     /locus_tag="VGA15_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591231.1"
                     /translation="MNDAILDVQGLIGGYGKMTILNGTSFSVPAGSITTLIGPNGAGK
                     STVFKAIFGLLKLREGRILFRGCDVTGLNPRQLLAAGICYVPQGRNIFPELSVRHNIE
                     LGAAAAERDITDLAARIEAALDRFPVLRRKATQQASTLSGGEQKQLEIVRGLLLNPQL
                     VLIDEPSIGLSPLMVQQTFAMLKELRDHGVSILMIEQNARSALEISDFGIVLELGQTR
                     LVDRAERVLGDPRIGQLFLGGAMTAANGE"
     gene            complement(16286..17071)
                     /locus_tag="VGA15_26250"
     CDS             complement(16286..17071)
                     /locus_tag="VGA15_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9591232.1"
                     /translation="MGRLRSLLIPGVDKQLLVRDAARVMPDLARRRFIAGGASFGALT
                     LLTGCNVSDSFSAEEMLKQISKFNDGVQAAIFNPYALAPTFPESAITKPFPFNAYYEV
                     DDAPNIDGKNWKLEVRGLVDNKKSWTLEELYKLPQEKQVTRHICVEGWSAIGSWTGTP
                     LRDFLKIIGADTRAKYCWFQCADSDGYNSPLDMASALHPQTQMSFKFADEILPRAYGF
                     PMKIRVPTKLGFKNPKYVISMEVTNDYKGGYWEDQGYNSFSGS"
     gene            complement(17095..17724)
                     /locus_tag="VGA15_26255"
     CDS             complement(17095..17724)
                     /locus_tag="VGA15_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX9591233.1"
                     /translation="MSSLAASGQHTVVTPAKVIQPAWVRTVHWINAVAIVLMIMSGWQ
                     IYNASPLFDFKFSSNITLGGWLGGALLWHFAAMWLLMVNGLVYLTLGFATGRFRKKLL
                     PITPAGVIADTKAALTFKLSHDDLTKYNNVQKLLYAGIIVVGIVIVLSGLSIWKPVQL
                     QYLTALFGGYDVARYVHFICMAAICAFLVVHVALALLVPKSLRAMIIGR"
     gene            complement(17912..18493)
                     /locus_tag="VGA15_26260"
     CDS             complement(17912..18493)
                     /locus_tag="VGA15_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX9591234.1"
                     /translation="MSKRIALFSAAAFAALALTAAVVAPVSAQDKMKSEMSGEKTVMV
                     GGAAMFPSKNIIQNAVNSKDHTTLVAAVKAAGLVETLEGKGPFTVFAPTNSAFGKLPA
                     GTVDSLVKPENKATLTKILTYHVVPGKLEASSLTDGKKLKTVEGEELTVKHQDGKVWI
                     VDAKGGTSMVTIANVNQSNGVIHVVDTVLMPAS"
     gene            19014..20414
                     /locus_tag="VGA15_26265"
     CDS             19014..20414
                     /locus_tag="VGA15_26265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9591235.1"
                     /translation="MSSITADGLAAAAAIPADDQIGPLEQRHLQFANYRAVTDGATVS
                     KTHWHIATANALGWGFDGMDGVIFALVSPMVIKEFSLTVPEYRSGLQIALFVGIAGLY
                     FWPWLSDRLGRRTLLAVNIALFSLLMPVVALSPTFAIFVVARSLVGFALNGEWSLGSM
                     LVAETWPARLRGRVISMTRSTWCLGASLAGGITGLVAADFGWRVAVMVPGVIALLAIY
                     VRATCPESPYWVRAQDRKQRIAKTLAGGGRVSDDDSAWFGKANSVGIRQVFLPDVLPA
                     TLVALFVACCSTCIFGTVGAWMPLYLATEKHWSTAEYSTFYVLWGISGFLGLCVAGWL
                     ADKIGRRLAFVLTLIEGAVFITLWVTTENHLLLWAFGLAWSFGFLGFWGPSTTLTAEV
                     FPTRIRGAANGVVWTIAYFVGFVLFPFVTVALQQHTGSFALSFLCIPVLMVAMAIGVT
                     IMVPEHSGKELNEIIV"
     gene            complement(20534..>20876)
                     /locus_tag="VGA15_26270"
     CDS             complement(20534..>20876)
                     /locus_tag="VGA15_26270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9591236.1"
                     /translation="TDVIDGCVVRQPKAYPVYDDSYRDHMAMIRRDLEQSYPSLHLIG
                     RNGMHKYNNQDHAMMTAMLTARNILAGERIHDIWEVNEDAEYHEAGASGAQAALASER
                     SVPLKAKTRAA"
BASE COUNT         3744 a         6411 c         6399 g         4322 t
ORIGIN      
        1 gactctttcg agcccggcgc gtggacgatg aactcccaca gcaccatgca gaacttgagc
       61 aacagcaccg cgaggctgat gacgaggccg aggtaaaacg gcgccatcag ccagcggctg
      121 ttgaagatca ggctttcgaa gccgttctcg acgcgcttca gcttcggatt gggtcgataa
      181 ggagctggcg gctcggtcat cgcgattccg gctcccgggc tccaaagctc tcacgccgcc
      241 tttttcttgg cgctgatcag cttgcggttg atcaggcatt cggcgatctg gaccgcgttc
      301 agcgccgcgc ccttgcgcaa attatccgac acgcaccaca gcaccaggcc gttgtccacg
      361 gtggggtcgg cgcggatgcg gctgatatag gtggcgtcct cgcccgccgc ctcgtaaggc
      421 gtgacgtagc cgcccggttc gtgcttgtcg atgacaaggc atcccggcgc attgcgcagg
      481 atgtcgcgcg cctcgtcggg cgaaatcgga ttttcgaatt cgatggtgac cgcttcggaa
      541 tggccgacga acaccggcac ccgcacgcag gtcgcggtaa gcttgatttt gggatcaaga
      601 atcttcttag tctccatcat catcttccac tcttccttgg tgtagccgtc ctccatgaac
      661 acgtcgatct cggggatcac gttgaaggcg atacgcttgg ggaatttctt gttgatcagc
      721 tcgctgttgg tatagaccgc cttggtctgc gaaaacagct catccatggc gtccttgccg
      781 gcgcctgaca ccgattgata ggtcgagacc acgacccgct tgatcctggc gtggtcgtgc
      841 agcggcttta acgccaccac gagctgcgcg gtcgagcaat tcggattggc gatgatgttc
      901 ttcttgacga agcccgccac cgcatccgcg ttgacctcgg gcacgatcag cggcacgtcc
      961 ggatccatcc gccacgccga cgagttgtcg atcacgacag caccggcggc gccgattttc
     1021 ggcgaccatt ccttcgacac cgcgccaccc gccgacatca ggcagatatc gacgtcggag
     1081 aaatcgtaat gctcgagcgc tttgaccttc agggtgcggt cgccatacga cacttcgacg
     1141 cccatgctgc ggcgtgacgc cagcgccacc acctcgtcag ccgggaattt gcgttcgtcc
     1201 agaatgttga gcatttcccg cccgacattg ccggtcgctc cgaccagcgc gactttgtaa
     1261 cccatcgttc actctccgaa gaaaaatcct ccgaccccgc tcgaagcgga aagggaagag
     1321 gcagcgcttc tatgcgagaa acgcccttaa gacaacgtta gtgtgcattc gggcgtttga
     1381 tgcaatcgtt tttgggccgc tgtgccactc tttacaatcc gacccagatt catcctgtct
     1441 tgaacagcct tgccgacgaa tgctgcggca tgctggcgcg tctcgttgcc tatttggggg
     1501 ctctggccct gcttgccatc gtcggcatcc atttgtggga cgaattgccg gcgacggaag
     1561 ccgttgggcg atccgccaga gcggactgga gcgtggccac gcgctcgcac ccggcgtttg
     1621 ccgtcagtca gttcgattta gccgaaaaaa cagagactta cgagattctc cggcaccccg
     1681 agggcggccg caaggatatc ttgagctggg ccgccccaga cggaaagcaa agcctaaaga
     1741 cggttgccga actcgaaatc tatcgccccg gcgatgaatc gagcggatcg ggtccgctgg
     1801 ccgacatcgc cgcccgaatc gaccccgaag gcttgcacga gctggaggcg gctggaatca
     1861 tcgacagcaa attcggcgcc gtgacgctgc ttcgcttcgc aggccaacca ggcaacgcgc
     1921 gatcctgcct cggattcatc aaacgtctcg aagaacccaa tctgcggatc tccggctgga
     1981 cctgccaggg cgacagcccg ccggcccgcc gcaccgcgat tggctgtatt ctgagccggc
     2041 tgattctgct cacggccgga aacgatccaa aactggctga attattcgcc cacgccgagc
     2101 tcaagcgggg cagctgcacc gcgtcagcca catcggcgga ctgggtgacc ggcgcggaaa
     2161 accccaggct gcgcggcact ttgtgaccga gctggccgag acggccagcc aagatgctgg
     2221 tttccatgca actttttcgt attccgacgc attattgtga tgcggtgtgg cccgattgac
     2281 cttgtggccg catacccccg gcagtaaaaa tggctgaaat cgatatggtg gcccggccgc
     2341 ccgtgaggat ctgatgacct ggaaattccc tgctgccaat aagcttgcgg tgttgctggc
     2401 cgccctctcc gctatcgccg ttggcttcgc cgtgacagcg gccaacgcgg agcccaagcg
     2461 gcagttcgat gagcgcggcc gcgcctacta cgggccgaac ggtcccaatg tgtcctatca
     2521 gcagggaccg cgcacgcgca tctacgttag caggcgctcc tggctcgatg ccggcgttga
     2581 agtgctgccc ggcgaccgca aattcaccga ttacgcgttt ccgacgccgt tcgggtaccc
     2641 atcgttcgcc cgcgagaaca acaatcgccc gattgaccgt cagccgctca atccgccgtc
     2701 tgatttgggc ggcgagccga ccaagttccc gctctattga ttttgacgag cactcgcgat
     2761 ccgtcgtccc ggcgaacgcc gggacccaaa atcgtttgcg tgtgtggtta aagaaggcat
     2821 ctgctccagc gacctaatcg cgagtacacg gagtatggat cccggcgttc gccgggatga
     2881 cgcaaggtgg cctgccccgc ctacccgtgg agcgcctgca actctttcaa gatcgcctcg
     2941 cccatctcta tcgttgagac cgccgtggtg ccctcggatt tgatatccgc ggtgcgcagg
     3001 ccgttcgcga gcaccgccgc gatcgcggaa tcgaccttgt cggccagcgc ccccatttcg
     3061 aacgaatagc gcagcgccat tgcgaacgac gcgatcatcg cgatcgggtt ggcgaggcct
     3121 cggccagcga tatcgggcgc cgagccgtgc accggctcga acagcgattt gcgctttttg
     3181 gtcttgacgt cgatctcgcc gagcgaagcc gacggcagca tgccgagcga tcccgtcagc
     3241 atcgccgcga tatccgagag catgtcgccg aacagattgt cggtgacgat gacgtcgaat
     3301 tgcttcggcc atttcaccag attcatgccg ccggaatcgg cgagctgatg ctcgagctgc
     3361 acgtccttgt attcgcgcgc atgcacctgg gtgaccacct cgttccagag cacgcccgat
     3421 ttcatgacgt tgcgcttttc catcgaggtc agcttgttgc ggcgcttgcg cgccagatcg
     3481 aacgcgaccc gggcgatgcg ctcgatctca taggtgtcgt agacttgggt atcgatggcg
     3541 cgcttctggc cgttgccgag atcggtgatg gttttcggct caccaaaata taccccgccg
     3601 gtgagttcgc gcacgatcat gatgtcgagc ccctcgacca cctcgcgctt caggctcgag
     3661 gcttccgcga gcgcgggata gcagatcgcg ggacgaaggt tggcgaacag cgccatgtcc
     3721 ttgcgcagcc gcaaaaggcc ggcttcgggc cgcacctcat agggaacgct gtcccatttc
     3781 ggaccgccga ccgccccgaa aatcaccgcg tcgctcgcct gcgccagcgc catggtggcg
     3841 tctgatattg cgaccttgtc ggcgtcatag gcgctgccgc cgaccagccc ctgctcggtc
     3901 tcgaaatgcg caatgccttg cgcgttgagc cagtcgatca ggcgcttcac ctccgccatc
     3961 acttcggggc cgatgccgtc gccggggagt agcagcagtt tgtgggtcgc catggttcgt
     4021 tacctacggt ttgtcgtcat tgccggcaaa cgcgccaacg ggtccgcgcg aagcgcggcc
     4081 cgatgacagg ctccgcgcgt ctttgcactg gatgacccgg caatccagct ttcatgaaga
     4141 agatgcatgc ccgggtcagg cccgggcatg acgacggata aggaaatccg cgcgcggagt
     4201 gctaaagcgg cattgcgcaa ttggcaagac accattgcgg ggggcgacct gtgcaatggc
     4261 cctggacccg gccatattga ggcccgccgg agtatccctt gcacgccccc aatcccacca
     4321 cttcctatgc gcatcccgcg cggctgatcc ttattctgtc gctggcgccc accgtctgcc
     4381 tcggcatcgg ccgcttcgcc tattccctgg tgctgccgga catgcgcgac gcgctgggct
     4441 ggtcctattc cgccgcgggt ttcatgaaca cgatcaacgc ggcggggtat ctcgccggcg
     4501 cgctggtcgc atcgaaactc atcaagcgcc ttggactatc cgccgccgtg cgcgggggga
     4561 cgctggcctg cctggtgtcg ctggcgctgt gcgcgctgtc gggcaatttt atcgttttga
     4621 gttttgcgcg gctgctcgca ggtgttggcg cggcggcggc attcgtcggc ggcgccgcgc
     4681 tggcggcgac aatcgcgcaa tcgcagggct cgcgggcgaa ttttctgctg agcctgttct
     4741 atgccggacc cgggctcggc attctgtcgt ccggcctgat cgcgccgttc gtgctgcagg
     4801 cgttcgggcc gggatcgtgg tggatcgtct ggtgggcgat gacgctgctt tcgcttctga
     4861 tgagcgttcc gctgctgctg gcgccgatcg ataccaaagc cggcatcgcc gatgcggcgg
     4921 cgccgaccag gttcgcgatt gggccggtca tgatttatct cgccggttat ttcctgttcg
     4981 gcgccggata catcgcctac atgaccttca tgatcgccta tgtgcgcgat gccggcggcg
     5041 gtgccgcggc gcagagcgca ttctggtgcc tgatcggcct gagcgcgttc gcaacgccat
     5101 gggtatggcg cggtgtgctg gcgcttgacc gtggcggcgt ctcaaccacg atcatcctcg
     5161 ccgtcaacgc ggtcggcgca agcctgccgc tgttcggaca ttccatggcg tggctcgccg
     5221 catcggcgct ggtgttcggt gtctcgttct tcgccattgt gggctcgacc accgccttcg
     5281 tgcgcttcaa ctacccgcct tccgcctggc ccaccgcgat cgccgccatg acgattgcct
     5341 tcggcgtcgg ccagatcctt ggccccatcg cgaccggcgc cattaccgac gcgctgggca
     5401 gcctgtccta tgccctcaat gccggggcgg cgatgctggc gctgggcgcg atcgcaacgg
     5461 cgtttcagcg aaggctggcg cgaaagtcaa agtcggcctg agcttcccct ctttcgcttg
     5521 cggttccgct gatttcgata tgctatccga cgagcccgct ctatcgccgg cgccggacgg
     5581 caacgctcga aggttttgcg caagggtatt cggcggcttt gggaacgtgc gcctccaggg
     5641 atttagcaca tgatgacgct ccgccaggtc gaggtcattc gcgcggtgat ggttacgggc
     5701 acgatcggcg gcgcggccaa actgctcaat gtgtcggcgc ccggcatcag ccgcctggtc
     5761 aagtacaccg agaagtcgct cggcatcaga ttcttccagc gccagaatgg acgctatttt
     5821 ccgacccctg aagcgcagag tatttttgag cagatcaacg gcgtctacaa gaaagtcgac
     5881 gatctcaccg agatcatttc aaagatcggt cgcggcgccc tgtcggaatt gcgcatcggc
     5941 tcggttccca gcatttctca aatcatggtg cctcgcgcga tcgagcgcgt tcggcggcgc
     6001 taccctgatc tgggtatcga catcaacatc ctcaaaatag aggaagcgat cgactatctg
     6061 atgctgggca aaggcgattg cgtcgccatg agttaccgac tggcccattc gggcctcgac
     6121 ttcatgccgc tggcgtccgg cgaactgttt tgcatcgtgc cgcaaggaca tgaactctcg
     6181 ggacggaaac aggtctccgc cgctgaaatg atccgctacc cactcatcgg aatcgacccc
     6241 aacgatccct atgggcgcat catggccgag atattcgcgc gcaataaact ggcctacgac
     6301 atcaccatcc gggcgcgttt cggaacaacc gtctgtgcgc tggtcaaggc aggtcttgga
     6361 atcgcgatca tcgatcaatt caccgtcgcg catggcggct atcccggcgt tgatctcctt
     6421 cgaattaccg agcccactcg attcgacact tacatcgcgg tgaaacgcgg cgcgtcgctc
     6481 tcgctccacg tcgaacattt catcgagtgc ttgcggtcgg aaatgcgtgc gattgaaccc
     6541 agccgaaatg ctcccggggc gccggtgtct cgcattcgaa aaaaataaca tggtgttatg
     6601 ttagcggcat aaatgagtaa ttgagttagg gagccgaaac gctatcgttt gtccgccttg
     6661 cggaaacgaa cgcctgcgcg agcaagctgc cgatgcgcgt cgggtgcagc gcgggacgct
     6721 tgcggtcgtc ggggctgtcc atgaagccgc gcgtagcggc gcccgcgtct cgccccggag
     6781 catcttctcg ccctggagca tcctggagca aatcgcatgg ccaagcgctc gatcgccact
     6841 gtctcgctca gcggcgcgct ggacgaaaag ctgcgtgcca tcgccgcagc cggattcgac
     6901 gcggttgaaa ttttcgagaa tgatttttta tcgtttggcg gaagccctcg cgacgtcggc
     6961 cagctctgtc gcgatctcgg ccttcaggtt tgcgcgttcc agccgttccg cgatttcgag
     7021 ggtatgcccg agccgcaacg cactcgcaat tttgcgcgag ccgaacgcaa attcgatgtg
     7081 atgcaggaat tgcagaccga cctgctgctg atatgcagca atgtttcccc ggcctcgctc
     7141 ggaggaatcg atcgggccgc cgccgatttt cgcgaactcg gcgagttggc tgcccggcgc
     7201 ggtctgcgcg tcggcttcga ggcgctcgcc tggggactgc acgtcaacga ttatcgcgat
     7261 gcatgggaga tcgtgcgacg cgccgaccac gacgcgatcg gtgtcattct cgacagcttt
     7321 catgcgctgg cgccatcgtt tccgatcacc gccattcaat ctattccagc ggacaagata
     7381 tttctggttc aattggctga cgcgccgaag cttggtctcg acatcctgtc ctggagccgg
     7441 catttccgat gttttccggg gcaaggcgat ctgcctgtgc tcggtttcat ggaggccgtg
     7501 atcgcgaccg gctatgcggg acctctctca ctcgaaatct tcaacgacca gtttcgtgcc
     7561 ggctcggctg cgcgcaccgc caccgacggg ctgcggtcgc tcatcctgtt gcaggatcaa
     7621 ctagcggcca acagcgccaa gggctcgccg gatgcactcg aaccgaaagc gcacagccgc
     7681 ggggtcgggt ttctggaatt tgccgtcaac gagacaaagg ctggcgatct cgccgacctg
     7741 tttggtcaac tcggctttcg caaaaccggc aagcatcgca gcaaggcggt cgaacggtgg
     7801 tcccagggca aggttgaact cgtcatcaat tgcgaggctg acggcttcgc gcattcgcat
     7861 tacatcaccc atgggccggg cgtgtgcgcc atcgccctgg atgtcgacaa cgcggaccac
     7921 atcatggcgc gcgcggaggc gctgaaggcg cgaacattct accaaccggt cgggccgggc
     7981 gagctggaga ttccggcgat ccacggcgtc ggcggaagcc tgctgtattt tctggatacc
     8041 gcgggaaaga actgggacac cgatttcgag gcgatccgga acagcgttac cgcggatcgt
     8101 ctacgcgccg tggatcacat ctcgcagtcg atgccttacg acgagatgtt gtcgtggctt
     8161 ttgttttaca ccgggattct cgatcttcaa cgtctgccgc agatggagat cgccgatccg
     8221 ctggggctgg tccagagcca ggccattgtc aataccgatc aaagcctgcg tattgttctc
     8281 aacgggtcgg ttgccacgcg aacattgtcc gcgcggttta tttcggagtt cttcggctca
     8341 ggggtccagc atatcgcctt ctcgtgcgac gatattttct taagcgttgc tgaaatgcgt
     8401 gcgcggggcg ccagcttctt ggaaattccc gacaactatt atgacgacgt cgaggccaga
     8461 tacgatctgg atgccgcgac gatggccgat cttcgggcca atcggattct gtacgaccgt
     8521 gaaggcgatg gcgaattctt ccagatctat acccacattt tcgacgaacg gttttttttc
     8581 gagatcgtgg agcggcgcac ctatcaggga ttcggtgccg ctaacgcccc gatcaggctt
     8641 gcggcgcaag cccgggaagt ccgcccgctg aacatccctc gcttctgatt agcaacgtgc
     8701 cgggcgaatt aacatgaggt tgggttcgcc accggctttg gtcattgtgg agcgaaggcc
     8761 ccgggactta tgattgctgc cgggtcgagg cccgcccagc gcgggaaaaa gccgcttcaa
     8821 agcggttaaa caaacgggaa ttcgggaggg cctacatgcg accgagaaat tcagttgcgt
     8881 tgctgggact actgcttgtc ggccttgaag gagctcacgg cgagaccgcg ggcaccgcgg
     8941 ttcgaattga taccggcgaa gtcgtcggca aattccagtt gaacgccgcc gtgcgcgcct
     9001 tcctcggcgt ccccttcgca gcgccccctg tcggcgcgct gcgctggaag ccgccgcaac
     9061 cggcagcgcc ctggccgaac gcgcgggccg cgacatccta tagcgcgcaa tgcatgcagc
     9121 cgacccgctc gaaaacatcg gtgtattatg aatatgccgg tggcgatcag ccgaccagcg
     9181 aggactgcct ttatctcaat gtctgggctc caagcgaaaa ggcggacgga aaactgccgg
     9241 tcatggtctg gatctatggc ggcggcttcc agcagggatc cgcggccaat cccgtcttcg
     9301 acggcgcgcg gctggctgcg cgcggcgtca tggtcgtttc catcaactat cgcgtgggga
     9361 tattcgggtt catggcccat ccggacctga ccgcggaatc cccgcagcac gcctccggaa
     9421 attacggcct gcttgaccag gttgccggat tgcagtgggt gaagcgcaat gccgcggcgt
     9481 tcggcggcga cccggataac gtaacgatct ttgggcaatc ggctggagcc gccgcggtga
     9541 cctatttgtt cacctcgccg ctggcgcgcg gattatttgc ccgcggcatt tgcgagagct
     9601 ttggcttcgc caacaagacc atgacgagtt tggcagacgc cgagaaaggt ggggcggctc
     9661 ttgctgaaaa agtcgccgca ggttcgctgg gcgatctgcg tcagataccc tcggtaaaac
     9721 ttcttgaaac gcatgtggcg atgagcccaa tcattgacgg ctggtttctg ccggccaatc
     9781 cttacacgat gttcgctcag ggcaaggaag cccctgtccc cctgctggcg ggatggaatg
     9841 ccgacgaagg cgcgacgttt ccgcatgcga aaacgcttgc ggcctatcag gaatggattc
     9901 gccagaaata tcccgacatt gccgatcgcc tgctggaact gtaccccgcg cgaactgacg
     9961 acgaggccaa gaccgccaac aagcgcatcg tacgggacag cctgttcgca tggggtccct
    10021 ggagtgccgc gcggttgcac gccaaaaacg gttttgccac ctatctgtac catttcaatc
    10081 atccccaacc acttcgagca ggtgtcacct atgacgaaat cgactctgtc gatggcctcg
    10141 gtacgttcca cagctcggaa tatccctatc tgttcggtac gctggacgta ttgaccagag
    10201 actggacgaa taccgatcgg atgctgtccg atcgcatgca atcctactgg gtcgcttatg
    10261 cctatagcgg cagccccaat gcatctggct tggcccgttg gccgtcgttc aacgaaaccg
    10321 ccgagaccac catgtatctg ggagacaaga ccggcccggg accggtaccg aaccttgata
    10381 gactcgaact gttcgacacc ctgccatcgc cttttggcgg cttctgaaag gcggaccgcc
    10441 gtgtatcaag gcgttacgtg cgcgtcatca gcgtgcacgc cgatccagac tgcggacaat
    10501 gctgcgcaaa ggtttcgacg actgagtcag tcgccgtgtg actccggcgt catgtgaaca
    10561 tcgaaaataa catcatgtta tgtttgcggc ataaatgagt aattgtgtta cactgcggaa
    10621 acgctatcgt ttgtcttact tttctggggc cgcataagac ttgctggtgc ctgtcagttg
    10681 cgcgagacat accgactgtg accaaacatc aaccttccac ccgtcggagc tttcttaccg
    10741 gtttgctcgg cgcgccgatc gcgcagtcgg ccgcgccggc catgcatgaa cgggctgccg
    10801 aggcgctcgg ctttcgctgc cattaccagc tcatcgaggt cgccggcgcc gggcgagagg
    10861 agttgaaggt cttgctcgaa ggcgttcggc ggctgggttt cgccggcatc aacgtcacct
    10921 ttccctacaa ggaagctgtc gtcgatctgc tcgacgcgtt atcgccgggc gcggcgctga
    10981 ttggtgcggt caataccgtc attgtccgcg acggccggtt gaccggccac agtaccgata
    11041 cgaccggttt tgcgcgcgcc attgctccgc tcgtcgcggc gtcgtcgcga ggtcccgtcg
    11101 ccgtgatcgg cgccggcggt gtcggcaagg cgattgcgtt tgcgctggcc ggcctcggcg
    11161 tcggcgaatt gcggatcttc gatagcgagc acgccaaggc catgcagctt gcggcacggc
    11221 tcgaaggcca cgtcagaacg aaagttaccc acaacgtcga ggacgcgctg cgtggtgtgg
    11281 caggcttggt caacggctcg ccggtcggga tgctgcccag ccgtgacacg ccggttccgg
    11341 atgctctgct gcatgccgga ctgtgggtcg ccgatgcggt ctattccccg ctctggacgc
    11401 cgctgttgac ggcggcgaaa gcaaagggcg ccacggtcat gaccgggcgc gagcttgcca
    11461 tttatcaagc cgccgacgcg ttcgaattat tcacggggtt ggcgccgtcc atcgccgaaa
    11521 tgggaattgc attcgacgac gtgatggaaa aaagatacgc cgctccgcac gctgcgtagc
    11581 aacacggccg acgatgcgat gataaatcgc acggtcacag ggggaaaccg aatgaagcca
    11641 ggaatcttga agcaaggaat cttggcgagc gccttgctcg caaccgtctt gaccggagca
    11701 gcgttcgcgc aatccggcgc gcccattaaa ctggccaacg tcgccgaact gtccggcggc
    11761 ggcgccaccg tcggcaccaa ctggaaaaac ggtatcgatc tggcgatcga agagatcaat
    11821 gccaagggcg gcgttctcgg ccgcaagctg gaagtcaccc acgccgattc acagtcgaac
    11881 cccggcgtgg cgcgcgcgca ggtgcagaag gcgctcgaca gcgagccgta cgtcctgctc
    11941 ggtcccggtt attcggggtc cgtcaaggtc acggcaccgc tcgcagccga agccggtatc
    12001 gcgcagatca tgggcggcga agccgccgaa ctgacacggg gcgacaacaa gtttctgttc
    12061 cgcacgtcgt tcggtcagca atcatcgatg ccaaaagtcg ccaaatacat caacgacgac
    12121 atcaaggcca aatcggtggc gatcgtctgg gtgaataacg acttcggcaa aggcggacgc
    12181 gacgtcatca ccggggaatt tgcgcgttac ggcatcaagg tcgccgccga tctgtccacg
    12241 gaggccggcc aggccgattt cgccgccgac gtgagcaaga tcaaggccgc cgcgcccgac
    12301 gctgttttca tctacctcaa tgaggaagaa agcgcgcgca tcctcaagga gctgaagcgg
    12361 caaagcgtga ccgcaccgct gatcggcgag acgacgctgg tcggccagaa ggtcgtcgaa
    12421 ctcgccggcg acgccgccaa cggcgcgcgc ggccatgtcg ggctgacgac cgacgcaccg
    12481 gtcgaactgg tcaaggcgtt ccgggaaaaa ttcgtcaaga agtacaacta cgttcccgat
    12541 cacaacggca tcaaaggcta tctcgcgatc tacatgatca aggcgaccac ggaaaaaatg
    12601 ggcaaggtcg atgccaaggc cttcgccgac aatctgcatg gcctcaccat caaggctgcg
    12661 aatgagccgg gcatcctgat ggatgtgacc ttcgatcaga agggcgacat cgatcgtcag
    12721 ggttttctgg tggagatcgt cgagggcaag caggtcatca agcaggtgct gccgaaactg
    12781 aattgagcca tgcgtctcca gccacgtcgc gcgaagtgta tctgatagcg tcaatcgaca
    12841 agggggccgg agaagcatgt ccaatctgct cgatcttctg ctggccggcc ttgcgaccgg
    12901 ggccatctac gcgatcgtcg ctgttggctt cacgctgctg tggcagacct cgcagaccat
    12961 caatttcgcg cagggcgaat tcgtcatgct gccggcgttt ttggtgctgg cggcgatgca
    13021 cgccggcgcg ccattctggc tcgcgatcct gatcggcatc ctgttgtcgc tgctgctgct
    13081 cgggctgggc ttcaagatgc tgctggtcga tccgatgctt cgccacggcg tgttgccgct
    13141 ggcgatcgca acgatggcgc tcgcgatcgg catcaaggag gccgtgaagc aattcttcag
    13201 cgccgaggcg tcgccgtttc cgtccgtagt gcccgccggt gacatccagg tcttcggccg
    13261 cctggtgtcg ttgcagagca ttggcgtgct ggcgctggcg atccttgccg tggtcggcct
    13321 gaccgcgttc ctgaaccgca cctcgatcgg ccatcagatg caggcggcgg cccagaaccc
    13381 gaccgtggcg cgcattatcg gcgtgccggt ggaacggatg atcctgctga ccttcctgat
    13441 caacgccttt ctggtggcgc tggcgtcgct cttgatcacg ccgatctatc tcgccaaatt
    13501 ttccagcggc gaagtgctgg gacaggcggc cttcatcgcg gcgatcgtcg gcggttttaa
    13561 tcaggtgcga ggtgcaattg ccgggggtct attgatcggc atcgtggaca atctctcggc
    13621 ggcctatgtc tcgacccact atcgcgcggc ggtgccgctg atcctgctga tcgtcatcat
    13681 cctgtttcgc ccgcagggaa tcctcggtcg tgccgaggag cgcacgatat gaccggcgcg
    13741 ggaagatatt ggcgcatcgc gctcggcgcc atcgtgatcg cagggctgat cgtggtgccg
    13801 atgaatttca atcgctacgg tctgttcatc ctgagccagt gggcggtgat gaccatcgcc
    13861 gccatgggac ttaatctgac gctcggctat gccgggcagg tctcattggc acagggcgca
    13921 ttcgtcggca tcggggccta tgccgccgca atcatgacct cgcagggcat gccgctgata
    13981 tcagcgctcg gcatcgccgt cgtcgtgtgt tttgccgtcg gctggatcct cggctatccg
    14041 gcgctgcgcg tgcagcacca ttatctggcc ttcgtgacgc tggcgttctc gacattggct
    14101 tttctcgtgt tccgtaacga ggactggctg accaagggca tctacggcat cagcaatatt
    14161 ccccggccgc acatcttcgg cgttgccacc aatcggccgc tgccgttcta ttacgtctgc
    14221 ctcggttcac tcggcatcgt ttcggtgatg ctgtggtggc tgatccgctc gccctggggc
    14281 cgcgcctttc aggcgctgcg cgagaatccg atcagggcgc tgtcgctcgg catcgatacc
    14341 cgccgctata cattgatggc ttttgcgatc ggttcggcgc tgggcggcgt tgccggtacg
    14401 ctgtacgcgc cgctgacgca atatgtcgat ccggtgccgt tcaacctgtc gctctcgctg
    14461 gacctcctga tgatggtgat cgtcggcggc gccggcttct tcttcgggcc gttcctgggc
    14521 gcgatgatcg ccgtgctgct tccggaatgg ctgcggttca cgcagggcta ttacctgatg
    14581 ctgtacgcca tcgccgtgat cctgctcttg atctattcgc cgaccggcat tttgggcatc
    14641 cttgatcgct ttctggcgga gcgccgcacc aaaacggcgt tcgcgctgcg cgccgtcgcc
    14701 gaatccagac tgggaacggc gccatgaccg cggtcctcga ggtcaccaac atcagcaaac
    14761 gttttggcgg catctcggcc gtcgatggcg tgagctttga cgttcacgag ggcgagattc
    14821 tcggaatgat cggaccgaac ggttgcggca agtcgacgct gttcaactgc attctcggcc
    14881 agctgacgcc gagcgatggc gaagtgaagg tcgacggcaa ggtcgtgacc ggctttcggc
    14941 cctccgatct caatcgcctc ggggtcagcc gcacattcca gttgctgcag gtgtttccaa
    15001 aactgtcggt gcgcgaaaat ctcattctcg caggacagga acaccagggc agcatgatgt
    15061 cccgcctgtt cggtccctcg gatgcggggt tgtccgaagc cgccgatcgg atgatcggtt
    15121 ttttcaagct tgatcatctc gccgacgagg ccgccggagg cttgtcctac ggtcagcaga
    15181 agctgctcga cgccgcgatg gctttcatgg gcgggccacg tctggtgctg ctcgacgagc
    15241 ccgccggggg cgtcaacctc accatgctcg tcgacctcag ggagcgcctg gccgccatca
    15301 atcgcgagaa gcgcgcgacc ttcgtggtca tcgaacacaa tatggaattc gtcatgtcgc
    15361 tgtgttcgag ggtcatggtg atggccgaag gccgggtgct ggcgatgggc aagcccgaag
    15421 aagtacgcgc caatcccgcc gtgatcgaag cctatctcgg ccattgagga ccagcccatg
    15481 gacctgccca tgaacgacgc cattctggat gttcagggcc tcatcggcgg ctatggcaag
    15541 atgacgatcc tcaatggcac cagtttctcg gtgccggcgg gctcgatcac cacgttgatc
    15601 ggtccgaacg gcgccgggaa atccacggtg ttcaaggcca ttttcggcct gctgaagctg
    15661 cgcgagggca ggatcctctt cagagggtgc gacgtgaccg gcctgaaccc gcgccaattg
    15721 ctggccgcgg gcatctgcta cgtgccgcaa gggcgcaaca ttttccccga actgtcggtg
    15781 cgccacaaca tcgagcttgg cgcggcggcg gccgagcggg acattaccga tctcgcggcg
    15841 cggatcgaag ccgcgctcga ccggtttccg gtgctgcgcc ggaaggcgac gcagcaggct
    15901 tccacgctgt cgggcggcga gcagaagcag ctcgaaatcg tccgtggcct gctgctcaat
    15961 ccgcaactgg tgctgatcga cgaaccatcg atcggtcttt cgccgctgat ggtgcagcag
    16021 acttttgcta tgctgaagga attacgcgac cacggtgtgt cgattctgat gatcgagcag
    16081 aatgcgcgat ccgcactgga gatctccgat ttcggcatcg tgctcgaact cggccagacc
    16141 cgcctggtcg acagggccga gcgggtgctg ggcgatcccc gcatcgggca attgttcctc
    16201 ggcggcgcca tgactgccgc gaatggcgag tagcgagggt gactcactat tcactattcg
    16261 ctattcgcca cccgccattt gccgttcaac ttccgctgaa cgaattgtag ccctggtctt
    16321 cccagtagcc gcccttgtag tcgttggtga cttccatcga gatcacatat ttcggattct
    16381 tgaagccgag tttggtcggc acccggatct tcatcggaaa accataggcg cggggcagta
    16441 tttcgtcggc gaatttgaac gacatctggg tttgcggatg cagcgcgctg gccatgtcga
    16501 gcggtgagtt gtagccatcg gaatccgcgc actggaacca gcaatatttg gcgcgcgtat
    16561 cggcgccgat tattttcagg aaatcgcgca acggcgtgcc ggtccagctt ccgatcgcgc
    16621 tccagccctc gacgcagata tggcgcgtga cctgcttttc ctgcggcagc ttgtagagct
    16681 cttccagcgt ccatgacttc ttgttgtcga caagaccccg cacctcgagc ttccaattct
    16741 tgccgtcgat gttgggcgcg tcgtcgactt cgtagtaggc gttgaacgga aacggttttg
    16801 tgatcgcgct ttccgggaag gtcggcgcca gcgcgtaggg attgaagatc gccgcctgga
    16861 cgccgtcatt gaactttgaa atctgcttca gcatctcttc ggccgaaaaa ctgtcagaga
    16921 cgttgcagcc ggtcagaagc gtcagcgcgc cgaagctggc gccgccggcg ataaagcggc
    16981 gacgcgccag gtccggcatc acccgggcag cgtccctgac cagcagttgc ttgtcgacgc
    17041 cgggaatcag aagcgagcga agacgaccca tgatgtgttc tccctaaaat tcattcagcg
    17101 gccgatgatc atggcgcgca ggctcttggg caccaatagc gccagcgcga cgtgcaccac
    17161 caggaatgcg cagatcgccg ccatgcagat gaagtggaca taacgcgcga cgtcgtagcc
    17221 gccgaacagc gccgtcagat attgcaactg caccggtttc cagatcgaaa gacccgacag
    17281 cacgatgaca atgccgacca cgatgatgcc ggcatagagc agcttctgca cattgttata
    17341 tttggtcaga tcgtcatgtg agagcttgaa ggtcagcgcc gccttggtat cggcaatcac
    17401 gcccgccggc gtgatcggca gcagcttctt gcggaagcgg ccggtggcaa aacccagcgt
    17461 gaggtagacg aggccattga ccatcagcag ccacatcgcg gcgaaatgcc agagcagcgc
    17521 gccgccgagc cagccgccaa gggtgatatt gctcgagaac ttgaaatcga acagcggcga
    17581 ggcgttatag atctgccatc ccgacatgat catcaggaca atcgcgacgg cattgatcca
    17641 atgcacggtg cggacccacg ccggttggat cacttttgcc ggggtgacca ctgtatgctg
    17701 gccgctggct gcaaggctgg acatattagt tccttggctg ttccgttctt gaacgtcgat
    17761 ataggcttat acgccgggaa gcgctgcttc gttactgcca taaaggctat caaactgatg
    17821 cccgcggggt atcgcggtca caaaaaagcg agccgggttg ccccggctcg ccctagcgca
    17881 tcctgttggg accaaatcag gacgcggggt gttacgaagc cggcatcagg acggtgtcga
    17941 ccacatggat cacgccgttg gactgattga cgttcgcgat cgtcaccatc gaggtacctc
    18001 ccttggcatc gacgatccag accttgccgt cctggtgctt caccgtcagc tcttcgcctt
    18061 caacggtctt gagcttcttg ccgtcggtca ggctggaggc ttcgagtttg cccgggacca
    18121 cgtgataggt caggattttg gtcagggtcg ccttgttctc aggcttcacc agcgagtcga
    18181 cggtgccggc cggcagcttg ccgaacgcgg agttggtcgg tgcgaacacc gtgaacgggc
    18241 ccttgccttc cagggtctcg accaggccgg cggccttcac cgccgccacc agcgtggtgt
    18301 ggtccttcga attgacggcg ttctggatga tgttcttcga cgggaacatc gcggcgccgc
    18361 cgaccatgac ggtcttctcg ccggacattt cgcttttcat cttgtcttgc gcactcacgg
    18421 gcgcaacgac ggcggcggtc agggcaagcg cggcaaaggc ggcggccgaa aaaagtgcaa
    18481 tacgcttcga catggatacg tctcccaatg gattgaggtc accggcggat atcgccggta
    18541 gctgaaagcc aacaacgacg ctgtcttatt gcttgattga ctgcgtcgcc gttgcgccga
    18601 gttacgggag gtttcggagc cggtttcgaa gaataattta tcgtgatgcg tgaaactatt
    18661 ttccgtggcc gccgtgtgcg actttcagtc gacctcatcc ctgcgtaacg gcttcgccgt
    18721 tatcgctgga ggtgcgagcc tattcggcga gcctcgaagg atgacttgcg acggtgtgcg
    18781 tggccatcct tcgaggccgc cgcaagcgcg acggcacctc aggatgacga cggcgcacac
    18841 gcggccccct ttctcaacac ttccccgcac ccatgtgcac tgccgcaagt attctgcttg
    18901 ccggttggtg gaacttggtc cagccttgag tatccgggca ccgcctccaa aaaaacgcgg
    18961 gccggcaaca ataacgcgcc gtcaacggtg cggcgcgaag cgggaggaca cgcatgagca
    19021 gcatcacggc agatggtttg gccgcggcgg cggctattcc ggccgacgat cagatcggac
    19081 cgctggaaca gaggcacctt caattcgcca attatcgcgc cgtcaccgac ggcgcaacag
    19141 tttcaaaaac ccattggcat atcgccactg ccaacgcgct cggctggggc tttgacggca
    19201 tggacggcgt gatcttcgcg ctggtgtcgc cgatggtgat caaggagttt tcgctcaccg
    19261 tgccggaata tcgctccggc ctgcagatcg cattgtttgt cggcatcgcc ggcctgtatt
    19321 tctggccgtg gctgtccgac cggctcgggc ggcgcacgct gctcgccgtc aacatcgcgc
    19381 tgttctcgct gctgatgccg gtggtcgcgc tgtcgccgac gtttgccatc tttgttgtcg
    19441 cgcggtcgtt ggtcggtttt gcgctcaacg gcgaatggtc gctcggttcg atgctggtgg
    19501 cggagacctg gccggcgcgg ctgcgcgggc gggtcatcag catgacgcgc tcgacctggt
    19561 gcctcggagc ctcgctggcg ggtggcatca cgggacttgt cgccgccgat ttcggctggc
    19621 gtgtcgccgt catggtgccc ggcgtgatcg cgctactcgc gatctacgtc cgcgccacct
    19681 gtccggaatc gccctattgg gtgcgcgcgc aggaccgcaa gcagcggatc gccaagacgc
    19741 tggccggcgg cggccgcgtc agcgacgacg acagcgcatg gttcggaaag gcgaactcgg
    19801 tcggcatccg ccaggtattt ctgccggacg tgctgcccgc cacgctggtg gcgttgttcg
    19861 tggcgtgctg cagcacctgc attttcggca cggtcggtgc ctggatgccg ctgtatctgg
    19921 cgaccgagaa acactggtcg acggcggaat acagcacctt ctatgtgctc tggggtattt
    19981 ccggcttcct cggcctctgc gtggcgggct ggctcgccga caagatcggc cgccggctgg
    20041 cattcgtgct gacgctgatc gagggcgcgg tcttcatcac gctctgggtc actaccgaaa
    20101 atcatctgct gctgtgggcg ttcggcctcg cctggagctt tgggttcctc ggcttctggg
    20161 gaccgagcac gacgttaacg gcagaggtgt ttccaacccg cattcgcggc gccgccaatg
    20221 gcgtggtttg gacgatcgcc tatttcgtcg gttttgtcct gttcccgttt gtcaccgtcg
    20281 cgctgcagca gcacaccgga tcgttcgcgc tgtcattcct gtgcatcccc gtgctgatgg
    20341 tggcgatggc gatcggcgtc acgatcatgg tgcccgagca ttccggcaag gagctgaacg
    20401 agattatcgt gtgaggtgac gacgaatgtg tcagcgcgtt tggcagtagc tgccattaaa
    20461 aactgactgg gccgtaaacg cgtaaatgcg ccgatcgggc tgactgcccg agtccgcttt
    20521 gtccccgata gcttcaggcc gcccgggtct ttgccttgag cggcaccgag cgctcgctgg
    20581 caagcgctgc ctgcgcgccc gatgctcccg cttcgtgata ttctgcgtcc tcgttgactt
    20641 cccatatgtc gtggatgcgt tcaccggcca gaatgtttcg cgccgtcagc atggcggtca
    20701 tcatggcgtg atcctgatta ttgtatttgt gcatcccgtt tcggccgatc agatgcaggc
    20761 tcggatagct ttgctcgagg tcgcgccgga tcatcgccat atggtcgcga taggaatcgt
    20821 catagacggg ataggccttg ggttggcgca ccacgcagcc gtcgatcaca tcggtg
//
LOCUS       DASWGM010000974         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_240542,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000974 DASWGM010000000
VERSION     DASWGM010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240542"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VGA15_26275"
     CDS             <1..957
                     /locus_tag="VGA15_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX9591237.1"
                     /translation="FFGAGAVLNSTGERDMDKLGGLIHRMPLTSFVFLVGCVAISALP
                     PFNGFVSEWLIFQAVLQSPQLPQWALKIMVPAVGALLALAAALAAACFVKAFGVTFLG
                     RPRSEAAETAQEVDKYSLSAMFILAVLCLLAGILPGLVIDALSPIATEILGGRMPIQA
                     NEPWLSIVPIAESRSSYNGLLVLVFITISASLAVYFIHRFASHALRRGPAWGCGFSDP
                     TPAAQYSGASFAQPIRRVFGTLVFRARDHVEMPAPGDIRPARLRIELHDLIWEGIYVP
                     LAGAVGFSADRLNRLQFLTIRRYLSLVFATLVTLLLVLAIWS"
     gene            948..1904
                     /locus_tag="VGA15_26280"
     CDS             948..1904
                     /locus_tag="VGA15_26280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX9591238.1"
                     /translation="MVMISDILVQGVQMLLVLLLAPLLTGFVRKIKARLVRRQGASIF
                     QPYRDLLRLLRKEVVLADDASWLFRVTPYITFAAIWVAAALVPTFATGLLFNWTADLI
                     AIVALLGSARFFLALAGMDVGTSFGGIGSSREVMIAALAEPAMLLFVFCMALVAGSTQ
                     LSTVAHFMASPGVGLRVSLGMAMIALIMVALAENARIPVDNPATHLELTMVHEAMVLE
                     YSGRHLAMIEFGAFLKLLLYISLIACVFLPWKIALFGTGPLSYVVGAGAYIVKLAVAG
                     FLLALFETATAKMRVFRVPQFLGAALMLGLLGTLLLFVSRSF"
     gene            1910..2572
                     /locus_tag="VGA15_26285"
     CDS             1910..2572
                     /locus_tag="VGA15_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-4 component E"
                     /protein_id="HEX9591239.1"
                     /translation="MHSLSFDVSHTLAGGLVLISFMMLYQDRLYSLLNVFTLHALVLA
                     LSVAWQAFVQNAPHLYITAAIALVFKAIVIPVALHRIVKQLGIHRDIESAVGIGPTML
                     AGMALVALSMVLMLRVTAEADPLAREDLAFALSVVLLGLLVMVTRRNAVSQVVGFMSL
                     ENGLVLAATGAKGMPLVVEISVAFSILIAFIVIGIFLFRIRERFDSVDVSALDDYRGE
                     RE"
     gene            2569..>3872
                     /locus_tag="VGA15_26290"
     CDS             2569..>3872
                     /locus_tag="VGA15_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEX9591240.1"
                     /translation="MSTASFDAVTAVLLIPIGSAALLAILPGYRLTARLNVAASLATF
                     LSALSLLVVERPQPMPYVLVDDLNIVFIVLNTFVGFTTSIFSASYIAHELETGRLTPV
                     YLRFYHAMYQTMMFGMNLAFVSNNIGLMWVAVEIATLTTVLMVGIYRTHAALEAAWKY
                     FILGSVGIAFALFGTILVYMAARPVVGEGQDAMVWTLLIQHAGDFDPALLNVAFVFLF
                     LGYGTKVGLAPLHAWLPDAHAEGPTPISAVLSGLLLNVALYALLRFKILLAANPAAIA
                     PGPLMVTMGLVSLVFAAFMLYRRRDIKRLFAYSSIEHMGIVVFAFGMGGPLANFAGLL
                     HMVMHSLTKSAIFYAVGHISQIKGTQRIGRIRGLTVTHPVLGWGLVMGVVAIAGLPPL
                     GIFMSEFLVVSSTFARQPLLAIVLVLGLLLAFGALMLRLTSVA"
BASE COUNT          543 a         1223 c         1199 g          907 t
ORIGIN      
        1 tttttcgggg ccggcgccgt cctgaactcg acgggcgaac gggacatgga caaactgggc
       61 ggcctcatcc accggatgcc gctgaccagc ttcgtgtttc tggttggctg cgtcgccatc
      121 tcggcgctgc cgccgttcaa cggcttcgtt tcggaatggc tgatcttcca ggccgtgctg
      181 caaagtcccc aactgccgca atgggcgctc aagatcatgg tgcccgcggt cggggcgttg
      241 ctggccctgg ccgcagccct ggcggcggcg tgtttcgtca aggcgttcgg cgtcacgttt
      301 ctcggacgac cgcgcagcga ggctgcggaa accgcgcagg aagtcgacaa gtactcactt
      361 tcggccatgt tcatcctcgc cgtactctgc ctgctggccg gaattctgcc gggactggtg
      421 atcgacgcgc tttcgccgat cgcgaccgag attctcggcg gccgcatgcc gatacaggcc
      481 aacgagccct ggctttcgat cgtgccgatc gcggagagtc gcagttcgta caacggattg
      541 ctggtgctgg tcttcattac gatatccgct tcgctcgccg tctatttcat tcatcgcttc
      601 gcttcgcatg cgttgcggcg gggcccggcc tggggctgcg gcttttccga tccgaccccc
      661 gccgcccagt attccggcgc aagcttcgca caaccgatcc gtcgtgtgtt cggcacgctg
      721 gtgtttcgcg cccgcgatca tgtcgagatg cccgcccccg gcgatatcag gccggcgcga
      781 ctgaggatcg aactgcatga tctgatctgg gagggaatct atgtgccgct ggcgggtgcg
      841 gtcggcttct cggccgaccg gctcaatcgt ctgcagttcc tgaccatccg gcgctatctc
      901 agtctggttt tcgcgaccct cgtcacgctg ctgctggtgc tcgcgatatg gtcatgatct
      961 cggacattct ggtgcagggc gtgcagatgc tgctggtgct gttgctcgcc ccgctgctga
     1021 ccggcttcgt gcgcaagatc aaggcccggc tggtgcgtcg ccagggcgca tcgatttttc
     1081 aaccgtatcg cgatctgttg cggctgctcc gcaaggaggt ggtgctggcc gacgacgcat
     1141 catggctgtt tcgggtcacg ccttacatca cctttgcggc gatctgggtc gccgctgccc
     1201 tggtaccgac tttcgcgacc ggcctcttgt tcaactggac cgcggatctg atcgccatcg
     1261 tagcgctact gggaagtgcg cgcttctttc tggcgctggc gggcatggac gtcggcacca
     1321 gtttcggcgg gatcggctcc agccgcgaag tcatgatcgc ggcgctggcg gagcccgcga
     1381 tgctcctgtt tgtcttttgc atggcgctcg ttgccggatc gacccaattg tcgacggtcg
     1441 cacatttcat ggcctctccg ggagtggggt tacgggtatc gctgggaatg gcaatgatcg
     1501 cgctgatcat ggtggcgctc gcagagaacg cgcgaatccc ggtcgacaat ccggccaccc
     1561 acctggaact taccatggtg catgaggcga tggttcttga atattcggga cgccatctgg
     1621 cgatgatcga gtttggcgcc tttctcaagc tgttgctcta catttccctg attgcatgcg
     1681 tgttcctccc atggaagatc gcgctcttcg gcaccgggcc attgtcgtac gtcgtgggcg
     1741 ccggcgccta tatcgtcaaa cttgctgtag cgggctttct cctcgcgctg ttcgagaccg
     1801 ccacggcgaa aatgcgagtc tttcgagttc cgcaatttct cggggcggcg ctcatgctcg
     1861 gcctgctcgg tacgctcttg ctgttcgtgt cgaggagttt ctgatgcaga tgcacagcct
     1921 ctccttcgac gtctcgcata cgctggccgg tggcctcgtc ctgatcagct tcatgatgct
     1981 gtaccaggac cggctttatt cgctgctcaa tgtctttacg ctccacgcct tggtgctggc
     2041 gctttccgtg gcctggcagg cctttgtcca gaacgcgcct catctttaca tcacggcggc
     2101 catcgccctt gtattcaagg ccatcgtcat tccggtggcg ctgcaccgca tcgtcaagca
     2161 gcttggaatt caccgcgaca tcgaatcagc cgtgggtatc ggcccgacca tgctggccgg
     2221 gatggcactg gtcgccctct cgatggtcct gatgctgcgg gtgaccgccg aggccgatcc
     2281 gctggcgcgc gaggacctcg ccttcgcctt gtcggtggtg ctgctcggac tgctggtcat
     2341 ggtgacccgc cgcaacgccg tcagtcaggt ggtgggattc atgtcgctcg agaacggact
     2401 ggtgctggcg gccaccggcg ccaagggcat gccgctggtc gtcgaaatca gcgtggcgtt
     2461 ctcgatcctg atcgctttca tcgtcatcgg catcttcctg ttccggatcc gtgaacgctt
     2521 cgactccgtc gacgtgtccg cgctcgacga ctaccggggc gagcgggaat gagcacggcc
     2581 tccttcgatg cggttacggc ggttctgctg attccgatcg gctcggccgc gttgctggcc
     2641 atccttccgg gctaccggct gacggcgcgc ttgaacgtgg ccgcgagtct cgcgaccttt
     2701 ctttcggcgc tctcgctgct ggtggtcgag cgtccgcaac ccatgcccta cgtgctggtc
     2761 gacgatctga acatcgtctt catcgtgctc aatacttttg tgggtttcac gaccagcatc
     2821 ttcagcgcaa gctacatcgc ccacgaactc gaaaccggcc ggctgacgcc ggtgtatttg
     2881 cgtttctacc acgccatgta tcagaccatg atgttcggca tgaacctcgc cttcgtgtcg
     2941 aacaatatcg gcctgatgtg ggtcgccgtc gaaatcgcga cgctgaccac ggtgctgatg
     3001 gtcgggatct atcgcaccca cgccgcgctg gaggcggcgt ggaaatattt catcctgggt
     3061 agcgtcggga tcgcgttcgc cctgttcggc accatcctgg tctacatggc ggcgcggccg
     3121 gtcgtcggcg agggccagga cgccatggtc tggacgctct tgatccagca tgccggggat
     3181 ttcgacccgg cgctgctcaa tgtcgccttc gtgttcctgt ttctcggcta cgggaccaag
     3241 gtcggcctcg cgccgctgca tgcctggctg cccgacgcgc acgccgaagg tccaaccccg
     3301 atatcggctg tcttgtcggg cctgctgctg aacgtcgcgc tctatgcgct attgcgcttc
     3361 aagattctgc tggccgccaa tccggcggcg attgcccccg gcccgctgat ggtgacgatg
     3421 ggcctggttt cgctggtgtt cgcggcgttc atgctctacc gccggcgcga catcaaacgg
     3481 ctgttcgctt attcctcgat cgaacacatg ggcattgtcg ttttcgcgtt cggaatgggc
     3541 ggcccgctcg ccaattttgc cgggctcttg cacatggtga tgcacagtct gaccaagtcg
     3601 gcgatcttct acgccgtcgg ccatatttcc cagatcaagg ggacgcagcg gatcggcagg
     3661 atccgcggct tgacggtcac ccatccggtg cttggctggg gcctggtgat gggcgtcgtc
     3721 gcgatcgccg gactgccgcc gctcggcatt ttcatgagcg aatttctcgt cgtcagctcg
     3781 acctttgcgc ggcagccgct gctcgcgatc gtgctggtgc tcgggctgtt gctggcgttc
     3841 ggcgcattga tgctgcgcct gaccagcgtc gc
//
LOCUS       DASWGM010000975         4972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2348843,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000975 DASWGM010000000
VERSION     DASWGM010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348843"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..752)
                     /locus_tag="VGA15_26295"
     CDS             complement(57..752)
                     /locus_tag="VGA15_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011648892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591241.1"
                     /translation="MLETRGLTAYYGDFQALYGIDTVIGRGETIAIIGANAAGKSSYL
                     KAITGLIRAEPDSVRFEDRPIGALGAANILKLGIAMVPEGRRLFSSLTVEENLLIGSY
                     GRHVVGIWSLDSIYGLFPVLKERRAAPSHTLSGGEQQMAAIGRALMSNPRVLLCDEIS
                     LGLAPIVIRNIYDAMPRIKQNGTSIVLVEQDIAQAMKVADRVYCFQEGRVSLSGRPTE
                     LTREQVHLAYFGR"
     gene            complement(761..1474)
                     /locus_tag="VGA15_26300"
     CDS             complement(761..1474)
                     /locus_tag="VGA15_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591242.1"
                     /translation="MLKIKGLRKSFGSVIAADDIDLSVAQGEALGIIGPNGAGKTTLF
                     NLIAGGLAPDAGEIHLNGRDITVARPHERCIAGIGCSHQIPRPFENLTVFENLLVAAV
                     HGGGLTERAAAAPCAEILERLHLLKRANTLAGALTLLERKRLEMARALATNPRLLLLD
                     EIAGGLSEGECLELVETIKQVHAAGMTIIWIEHIVHALVAVVGKLSVLNFGKKVAEGD
                     PLEVMASAEVRQFYIGLLA"
     gene            complement(1560..2876)
                     /locus_tag="VGA15_26305"
     CDS             complement(1560..2876)
                     /locus_tag="VGA15_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591243.1"
                     /translation="MTRIVLTGIDRRALLKSSAAAGAALATGIAMPAIAQTRPIKLGY
                     VSPQTGPLAAFAEADNFIIANFREAAKAGVKLGNATVPVEVVVKDSQSNPNRAAQVAK
                     DLIVQDKIDLMLVASTPETTNPVSTQCEIEEVPCISTVAPWQPWFIGRQADPAGGPPA
                     WKNFNYSYHFFWGLEDVIAVFTNMWSQVPTNKSVGGLFPNDGDGNAWGDKQVGFPPVL
                     EKAGYKLADPGRYQNLTDNFSAQISAFKNANAEIITGVVLPPDFTTFWKQALRQGFKP
                     KVASIGKAILFPVAVEALGKDGNNLSSEVWWSPNHPFKSSLTGMSAKDLAASYEAATK
                     KQWTQPIGFVHALFEVAVDALKRAPQRDAKAMVAAIAATNLDTVVGKVQWNGANLPPF
                     AAKNVAKTPLVGGQWRVKDGNKYDIVITDNRTAPNIPAGGKMEVIG"
     gene            3083..3832
                     /locus_tag="VGA15_26310"
     CDS             3083..3832
                     /locus_tag="VGA15_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX9591244.1"
                     /translation="MTTLAKGLAILSAFGKQRPTMTLSDAATVANLSRAAARRVLRTL
                     AQLGYVVQDGRAFSLTPRVLDLGFAYLSGQDWIDRALPMMKEVSEQLGESCSGSILQG
                     IEIVYVARVPARRIMSTALAVGSRLPAFHTSLGRAQLGCLDGAEIWRRLRSAPIEAYT
                     PYTITDMQALFDRVCADREQGFSIVDEELERGLRSIAVPVLDRSGQAVGAINLSAHST
                     RTTRNEMREKFLPELKAIAQRISQAPRPVQD"
     gene            3974..>4972
                     /locus_tag="VGA15_26315"
     CDS             3974..>4972
                     /locus_tag="VGA15_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9591245.1"
                     /translation="MAKTTKTAGGSTAGATPAKATMAKTNGGGDRVAFARRPENIGQM
                     EVSRPQTGAEYIESLRDGREVFIYGERVEDVTTHPAFRNTARMVARLFDALHDDKRKK
                     KLLLPTDTDNGGMTHAFFKAPKTVEDLIAGRDAIAEWARMTYGWMGRSPDYKAAFLAT
                     LGANMNFYDPYQDNARRWYKFSQERVPFINHAIIHPPIDRDRPPNEVSDVCCHVDKET
                     DAGLIISGAKVVATGSVLTNYTFVAHHGLIPVQDKKFAAVFMLPTNSPGVKFICRTSY
                     EMSSTVMGSPFDYPLSSRLDENDAVFIMDKVLVPWENVFVYGDLEKANNFFPRTGFL"
BASE COUNT          949 a         1560 c         1476 g          987 t
ORIGIN      
        1 atacaggccg ccaagcagaa cgccctggat gatcgtgtcg agccaatcga gcatgctcaa
       61 cgtccgaaat aggcaaggtg aacctgttcg cgtgttagtt ccgttggccg ccctgacagc
      121 gacaccctac cctcctggaa acaatagaca cggtcggcga ccttcatcgc ctgcgcgatg
      181 tcctgctcga cgagcacgat gctggtgcca ttctgtttga tccgcggcat cgcatcgtag
      241 atatttcgga ttacaatcgg cgccaagccg agacttatct cgtcgcataa cagcacgcgc
      301 gggtttgaca tcaacgcgcg cccgatcgcg gccatctgtt gctctccgcc ggacagcgtg
      361 tggctcggcg cggcccggcg ttcttttagc accgggaaca gcccatagat gctatccagc
      421 gaccatatcc cgaccacatg ccggccgtag cttccgatca gcaggttctc ctcaaccgtc
      481 agcgaggaga acagccgcct cccctccggc accatcgcga tcccgagctt gagaatattt
      541 gccgcgccaa gcgcgccgat gggcctgtcc tcgaaccgca cgctatcggg ctcggcgcgg
      601 atcaggccgg tgatggcctt gaggtaggag gacttgcccg ctgcgttggc gccgatgatc
      661 gcaatcgtct cgcctcggcc gatcacagtg tcgatcccgt acagcgcctg gaagtctccg
      721 tagtaagcgg taaggccgcg ggtctcgagc attggatgtc tcacgccaac agaccgatat
      781 agaattgccg aacctccgcg gaagccatca cctccagcgg atcgccctcg gcgactttct
      841 ttccgaaatt caatacgctc agctttccga ccaccgccac cagcgcgtgc acgatatgct
      901 cgatccagat aatggtcatt cccgcggcat ggacttgctt gatggtctcg acaagttcga
      961 ggcactcccc ctcgcttagt cccccggcga tctcatcgag cagcaacaaa cgggggttgg
     1021 ttgcgagcgc gcgcgccatc tccagccgct tgcgttcgag caatgtcagt gcaccggcaa
     1081 gcgtgttggc gcgtttgagc agatgcaagc gttcaagaat ttccgcacag ggcgcggcgg
     1141 cggcgcgctc cgtcaagccc ccgccatgca cagcagcgac cagcagattc tcgaacaccg
     1201 tgagattctc gaacggccga gggatctgat gtgagcaccc gattccggcg atgcagcgct
     1261 catgcggcct ggccacggta atatcacgac cgttcaggtg gatttcgccc gcatcgggtg
     1321 cgagaccgcc ggcgatcagg ttgaaaagcg tcgtcttgcc ggcgccattc gggccaatga
     1381 tcccaagcgc ctcgccctgc gcgaccgaca gatcgatatc gtccgccgcg atgaccgaac
     1441 cgaagctctt gcgcaggcct ttaatcttca gcatgcgctt gagtttaaca acacgacgga
     1501 cgaatcgaaa tgtaatcgca aacctgcgcc gagcatgctc cgctcgcttc gtcctgcttt
     1561 caaccgatca cctccatttt gccgccggcc ggaatgtttg gcgcagtcct gttgtcggtg
     1621 atgacgatgt cgtatttatt accgtccttc acgcgccatt ggccgccgac cagcggtgtc
     1681 tttgcgacgt tttttgcggc gaatggtggc aggttcgctc cgttccactg taccttgccg
     1741 accacggtat cgagattggt cgcggcgatc gcagcgacca tggctttggc atcgcgctgc
     1801 ggcgcacgct tcagcgcatc gacggcgacc tcgaacagcg catgcacgaa gccgatcggc
     1861 tgcgtccatt gtttcttggt cgcagcctca tagctggcgg cgagatcttt cgcactcatg
     1921 ccggtgagtg acgatttgaa cggatggttc ggcgaccacc acacttccga tgaaaggttg
     1981 ttgccgtcct tgccgagcgc ctcgaccgcg accgggaaca ggatcgcctt tccgatcgag
     2041 gcgaccttcg gtttgaagcc ttgccgcagc gcctgcttcc agaatgtcgt gaagtcgggc
     2101 ggcaacacga cgccggtgat gatctcggcg ttggcgttct tgaacgcgct gatctgcgcc
     2161 gagaagttgt cggtgagatt ctggtagcgg ccgggatcgg cgagcttgta accggctttc
     2221 tccagtaccg gcgggaatcc gacctgcttg tcgccccagg cattgccgtc gccgtcgttg
     2281 ggaaacagac cgccgactga tttgttggtc ggcacctggc tccacatgtt ggtaaagacc
     2341 gcgataacgt cttcgagccc ccagaagaag tggtagctgt aattgaaatt cttccatgcc
     2401 ggaggtccac cggccggatc ggcctggcgg ccgatgaacc agggctgcca tggcgccacc
     2461 gtcgagatgc agggaacttc ctcgatctcg cattgcgttg aaactgggtt cgtggtttcg
     2521 ggcgtcgagg caaccagcat caggtcgatc ttatcctgga cgatcaaatc ctttgcgacc
     2581 tgggcggcgc ggttgggatt tgactggctg tccttgacga cgacttccac cggaacggtc
     2641 gcgttgccaa gcttgacgcc ggccttggcg gcttccctga aattcgcgat gatgaagttg
     2701 tcggcttcgg cgaacgcagc gagcgggccg gtctgcgggc tgacgtagcc gagttttatc
     2761 gggcgcgtct gcgcgatggc gggcatcgcg atcccggtag cgagcgctgc gccggccgcc
     2821 gctgacgatt tgagcaacgc gcgacgatca atccctgtga gcacgatcct ggtcatgaag
     2881 cctcctccct gaaaaactcc gcccggcggc caggtctcgg ctgagccttg accgcacccc
     2941 cgaaattgtt cgatattcga actcgcgttc gctttttccg cgagtgtcaa tgagcgcctt
     3001 gacggtgtca accgctcgct agggcacggt gtgtacgatg atgccttccg gaccgccaga
     3061 cgagggtcgt gacaaggagt tcatgaccac gctcgccaag gggctcgcca tcctcagtgc
     3121 gttcggcaag cagcggccca caatgacctt gtcggacgcg gccacggtcg ccaacctctc
     3181 gcgggcagcc gcgcggcgtg tcctgcgcac gctggcccag cttggctatg tcgttcagga
     3241 cggccgtgcg ttttcgttga ccccgcgcgt tctcgatctg ggttttgcct atctctcggg
     3301 acaggactgg atcgaccgcg cgctgccgat gatgaaggaa gtgagcgagc agcttggcga
     3361 atcctgctct ggctcgattt tgcaaggcat cgagatcgtc tacgtcgcgc gcgtgcctgc
     3421 ccggcgcatc atgtcaaccg ccctggcggt tggcagccgg ttgccggcct ttcacacatc
     3481 gctcggccgc gcgcagctcg gctgtcttga tggcgcggaa atctggcgcc ggctacggtc
     3541 ggcgcccatc gaagcctata cgccttatac gatcaccgac atgcaagcgc tgttcgatcg
     3601 cgtttgcgcc gaccgggagc agggattttc cattgtggac gaagagcttg agcgagggct
     3661 ccgctcgatt gccgtcccgg ttctcgaccg ctccggccag gcggtcggcg ccatcaatct
     3721 ctcggcgcac agcacgcgga caacgcgcaa cgagatgcgc gagaaattcc ttcccgagct
     3781 caaggccatc gcgcaacgga tttcgcaagc acctcggccg gtacaggact agagcaattc
     3841 gttttgcgat tgctcgcttg tcaggacacc acgaagacct taacattccc gttcgatttc
     3901 cgcacagaag ttcgcatatc gaacataaca ggtgcggacg gtcaggcgat cgaaaattct
     3961 acgggaggtc acgatggcaa aaacgacgaa gacggcgggg ggaagtacgg ccggcgcaac
     4021 accagccaag gcgacaatgg cgaaaaccaa cggcggcggc gaccgcgtag cgttcgcacg
     4081 gcgccccgaa aatatcggcc agatggaagt tagccggccg cagaccggcg cggagtatat
     4141 cgaatccctg cgcgacggcc gcgaggtctt catctatggc gaacgcgtcg aggatgtcac
     4201 gacccacccc gccttccgca acaccgcacg gatggtggcg cgcctattcg atgccctgca
     4261 tgacgacaag cgaaagaaaa agctgctgct gccgacagac accgacaacg ggggaatgac
     4321 gcacgccttc ttcaaggcgc caaagaccgt ggaggatctc atcgccggcc gcgacgccat
     4381 cgccgaatgg gcccgcatga cctacggctg gatgggccgc tcgccggatt acaaggcggc
     4441 gtttctcgcg accctcggcg ccaatatgaa cttttacgat ccgtatcagg acaatgcccg
     4501 acgctggtac aaattcagcc aggagcgcgt gccgttcatc aatcatgcca tcatccatcc
     4561 gccgatcgat cgcgaccggc cgccgaacga ggtcagcgat gtctgctgcc acgtcgacaa
     4621 ggagaccgac gccggcctca tcatctcggg cgcgaaggtg gtcgccaccg gctcggtgct
     4681 gacgaactat acgtttgtgg cccaccacgg attgatcccg gtgcaggaca agaaatttgc
     4741 cgcggtgttc atgttgccga ccaactcgcc gggcgtgaaa ttcatctgcc gtacgtctta
     4801 cgagatgagt tcgacggtca tgggtagtcc gttcgactat ccgctatcga gccgactcga
     4861 cgagaatgac gcggtcttca tcatggacaa ggtgctggtg ccttgggaaa acgtattcgt
     4921 ctacggcgac ctcgagaagg caaacaactt ctttccgcgc accggcttcc tg
//
LOCUS       DASWGM010000976         1950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1484626,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000976 DASWGM010000000
VERSION     DASWGM010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484626"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VGA15_26320"
     CDS             complement(<1..571)
                     /locus_tag="VGA15_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX9591246.1"
                     /translation="MMPHVLTRSLDRSATVFLIVVAVVGALIPLSNLLLPEGSLFHVP
                     TYLMALFGKYVCYAVLALAIDLIWGYCGILSLGHGAFFALGGYAMGMYLMRQIGSRGV
                     YGNPVLPDFMVFLNYPKLPWYWHGFEMFWFAALMVILVPGLLAFCFGWLAFRSRVTGV
                     YLSIITQAMTYALLLAFFRNDFGFGGNNGL"
     gene            complement(774..>1950)
                     /gene="urtB"
                     /locus_tag="VGA15_26325"
     CDS             complement(774..>1950)
                     /gene="urtB"
                     /locus_tag="VGA15_26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX9591247.1"
                     /translation="THDETMLPVIDGALQKETNKAAKLAFTEARAAILLFKEDATDAE
                     KLEAIATIKARGDQEALALLTGLTGDQPPAIARAAANATSAIQGNLALWSMVQNAWYG
                     LSLGSVLLLAAIGLAITFGVMGVINMAHGEMVMIGAYVTFVVQQTIRTSFPGLFDYSL
                     LMAVPLAFIVAGAIGILIERSIIRFLYGRPLETLLATWGLSLVLQQAVRTMFGPTNQE
                     VGNPSWMSGAFEIGQITITYNRLWILCFTLAVFVILLAMLRYTSLGLEMRAVTQNRRM
                     AASMGIATSRVDALTFGLGSGIAGIAGVALSQIDNVSPNLGQSYIIDSFMVVVFGGVG
                     NLWGTLVGAFTLGIANKFLEPVAGAVLGKIAILVLIILFIQKRPRGLFALKGRAVEA"
BASE COUNT          411 a          624 c          610 g          305 t
ORIGIN      
        1 tgaggccgtt gttgccgccg aaaccgaaat cgttgcggaa gaaggccagc aacaaggcgt
       61 aggtcatcgc ctgggtgatg atcgagagat agacgccggt gacccgcgag cgaaacgcca
      121 gccagccgaa gcagaatgcc aatagccccg gcaccaaaat gaccatcagg gccgcgaacc
      181 agaacatctc gaagccgtgc cagtaccatg gcagttttgg atagttcagg aacaccatga
      241 agtccggcaa caccggattg ccgtagacgc cgcggctgcc gatctggcgc atcagataca
      301 tgcccatcgc atagccgccg agcgcgaaga acgcgccatg gccgagcgag agaatgccgc
      361 aatagcccca gatcaaatcg atcgcgagcg ccagaacggc gtagcagaca tattttccga
      421 acagcgccat cagataggtc ggcacgtgga acagggagcc ttccggcagc agcaggttgg
      481 acagcggaat cagcgcgccc acgaccgcca ccacgatcag gaacaccgtc gcgctgcgat
      541 ccagcgagcg cgtgagcacg tgcggcatca ttgttgttgt cccagccaca cggctcggtc
      601 cgccccctct ccccgtgggg gagagggggt tgcgacgcca gttgcttgag caagcgcccc
      661 ctcacccgcg ccggagcctg tcatcgggcg cgcccttggc gcgacccgtt gggcgcgacc
      721 tctcccccac ggggagaggt gaagaaacgc cgccgcaacg ttcgaaatcg gcatcatgct
      781 tccaccgccc gccccttgag cgcgaacagg ccgcgcggac gtttctggat gaacaggatg
      841 atgagcacca ggatcgcgat cttgccgagc acggcgcccg cgaccggttc caagaacttg
      901 ttggcgatgc cgagcgtgaa cgcgccgacc agcgtgcccc agagattgcc gaccccgccg
      961 aacaccacca ccatgaagga gtcgatgatg tagctctggc cgagattggg actgacattg
     1021 tcgatctgcg acagcgccac gccggcaatc ccggcgatgc ccgagccgag gccgaaggtc
     1081 agcgcgtcga cccgcgaggt cgctatgccc atggaggccg ccattctgcg gttttgcgtc
     1141 accgcgcgca tctcaagtcc gaggctggta tagcgcagca tggccagcag gatgacaaac
     1201 accgccagcg tgaagcaaag aatccacagc ctgttatagg tgatcgtgat ctggccgatc
     1261 tcgaacgcgc cgctcatcca ggagggattg ccgacctcct ggttggtggg accgaacatg
     1321 gtgcgcacgg cctgttgcag gaccagcgac aggccccagg tcgccagcag ggtctccagc
     1381 gggcggccat agagaaagcg gatgatgctg cgctcgatca ggatcccgat cgcgccggcc
     1441 acgatgaagg ccaatggcac ggccatcagc agcgaatagt cgaacagccc gggaaaactg
     1501 gtgcggatgg tctgctgcac gacaaatgtg acataagctc cgatcatcac catctcgcca
     1561 tgggccatgt tgatgacgcc catcaccccg aaggtgatgg ccagaccgat cgcggcgagc
     1621 agcagaaccg agccgagcga cagtccgtac caggcgtttt gcaccatgga ccacagcgcg
     1681 agattgccct ggatggccga ggtcgcattg gccgcggcgc gcgcgatcgc gggcggctga
     1741 tctccggtca acccggtcag caaagcgagc gcttcctggt cgccacgcgc cttgatggta
     1801 gcgatggctt caagcttttc ggcatcggtg gcgtcttcct tgaacaacag gatcgccgcc
     1861 cgcgcttcgg taaaggccag cttcgcggcc ttgttggttt ccttctgcag cgcgccgtcg
     1921 atcaccggca gcatggtctc gtcgtgggtc
//
LOCUS       DASWGM010000977         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2210499,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000977 DASWGM010000000
VERSION     DASWGM010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2210499"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VGA15_26330"
     CDS             <1..691
                     /locus_tag="VGA15_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX9591248.1"
                     /translation="LHLWVEAIKWATTRRGLLSLSPTMVYCFWHSGETTESSDLQLTF
                     TPASYKEGVQGQLEDEPGMTVASWQQRPESRGYVRARSADPFEPPIIQTNYLVAELDR
                     RVVVAGMKLARRLLASAPLAPYYAYEDFPGPDVQTDDEFLAAATQRGTTTFHPGCTCR
                     MGPADSSWAVVDDQLRVHGLQNLRVIDASIMPRMISANLNASTMMIADKASDLIRGRK
                     LPEAAIPARRA"
     gene            927..1574
                     /locus_tag="VGA15_26335"
     CDS             927..1574
                     /locus_tag="VGA15_26335"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX9591249.1"
                     /translation="MGAAAIALSRTFCLAAAWFDITSLQARVVRGLAIDAPRPMLHMV
                     LQVNLPEGLFLADVGFGNLAPTAPLVLSPDFEQDTPHEVMRFIRMGDELTLQSRLGDR
                     WEHIYRVVLLPRVDAEYEICNWFTASHPDSPYRSNLIAARPGSNRTRITLFNARLNVR
                     HANGEVERRTLNDKAEYLDVLAGTFGLALTDADLAAALETVERKGTRGAPHPFFA"
     gene            1694..2305
                     /locus_tag="VGA15_26340"
     CDS             1694..2305
                     /locus_tag="VGA15_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX9591250.1"
                     /translation="MLYEFATLSFHPLHGRQVTAGAEAYVIETEARGHLLGCWTTDIG
                     TIGRMFVLRGFADAAELAAERKRALFSANPFGAAEVLTGLEMDSYAPFPFLPPVVPGK
                     YGKVYEFRTYHLKPGGLTPTIAAWEAAMPERAKLSPLVINMYALDGAPRITHIWPYAS
                     LDARAAIRADSYAQGIWPPKNAPEQFYHATSTIGLPTAASPLA"
     gene            complement(2331..>3185)
                     /locus_tag="VGA15_26345"
     CDS             complement(2331..>3185)
                     /locus_tag="VGA15_26345"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX9591251.1"
                     /translation="MLQRSPTYFRTGRNAIDIAETLRALQVDETWIHEITRRKILYDQ
                     AVFTRKTFEEPEAAKKDLLSAVEAYLGPDYDIATHFTPKYRPWRQRIAFIPDGDLFQG
                     INSGKASVVTDEIDRFTETGILLKSGKTLEADIIVTATGFNLSANGDMEFCIDGKPLD
                     FADTVTYRGMMFTGVPNLVWVFGYFRASWTLRVDLVADFVCRLLNHMKETGAKKVTPA
                     LRAEDHNMPLLPWIDPENFNPGYMMRGMHLLPKRGDKPEWQHNQDYWAEKDEFPAIDL
                     GDRAFVYE"
BASE COUNT          599 a          968 c          985 g          633 t
ORIGIN      
        1 actccatctg tgggtcgagg cgatcaaatg ggccacgacg cggcgcggtc tgctctcgct
       61 gtcgccgacc atggtctatt gcttctggca ctccggcgag accaccgaga gctccgatct
      121 gcagctgacc tttacgcccg caagttacaa ggagggcgtg caggggcagc tcgaagacga
      181 gcctggcatg acggtggcgt cctggcagca gcggcccgaa agccgcggct atgtccgcgc
      241 ccgctcggcc gatccgttcg aaccgccgat catccaaacc aattatctgg tcgcggagct
      301 cgaccgccgc gtcgtcgtcg ccggcatgaa gctcgcccgg cggctgctcg catcggcgcc
      361 gctcgcgccg tattacgcat acgaggattt tcccggacct gacgtgcaga ccgacgatga
      421 atttctcgcc gcggcgaccc agcgcggcac cacgacgttt caccccggat gcacctgccg
      481 catgggcccg gccgacagca gctgggcggt ggtcgacgac caactccgcg tccatgggct
      541 gcaaaacctg cgcgtgatcg acgcctccat catgccgcgg atgatctcgg ccaatctcaa
      601 cgcctcgacc atgatgatcg ccgacaaggc gtcggatctc attcgtggcc gcaagctgcc
      661 ggaggcagcg atacccgcga gacgagctta gctatttgaa ttttcgattt gattgaaagg
      721 acaattcagc gtgtccgtgg acaattttga tcaagacgcc tggttaggcc gtatcgggta
      781 tgagggatcg cgcgtaccgg tgcttgagac gttgcgggga atggtcgagg ctcattccgc
      841 agcgatttcc tacgaaagca ttgatgtgct gctggacagg ccgccgaagc tcgacctcga
      901 ctcgctgcaa cggaagatga tcgcgggtgg gcgcggcggc tattgctttg agcagaacat
      961 tctgcctcgc ggcggcctgg ttcgatatca cgagcttgca agcccgtgtc gtgcgcggac
     1021 tggcgatcga cgccccgcgt cccatgctgc acatggtgct tcaggtgaat ttgccggaag
     1081 ggctgtttct cgccgatgtc ggtttcggca atctggcacc gaccgcgccg ctcgtgttgt
     1141 ctcccgattt cgagcaggat acgccgcacg aagtcatgcg cttcatccgg atgggtgacg
     1201 aactgacgtt gcaatccagg ctaggcgatc gatgggagca catctatcgc gtggtgctgc
     1261 tgccgagagt ggatgccgag tacgagatct gcaactggtt cacggccagt catcccgata
     1321 gcccgtaccg cagtaatttg attgcagcgc gtcccggatc caacaggacc cggattaccc
     1381 tgttcaacgc gagactgaat gtacggcatg caaacggaga ggtcgaacgg cgaaccctca
     1441 atgataaggc tgaatatctc gatgttctcg ccgggacatt cgggcttgcg ctaactgatg
     1501 ctgatcttgc cgccgcgctt gagaccgtcg agcggaaggg cacgcgcggg gcgccacatc
     1561 cttttttcgc ttgagcagcg tacgcttatc gaggaaatga catgcacgac aacgcgtgct
     1621 cagagctgct gcgtggccgc agtcccgcga catggcaaga tcatgaaaat gcattcccga
     1681 acgaggaccg ctcatgcttt atgaattcgc cacgctctcc ttccaccccc tgcatggccg
     1741 gcaggtaacg gctggcgcgg aggcttacgt gatcgagacg gaggccaggg gccatcttct
     1801 tggttgctgg accaccgaca tcggtacgat tggccgcatg ttcgtgctgc gcggattcgc
     1861 tgacgccgcc gaactggcgg ccgagcgaaa gcgggcgctg ttcagcgcaa atccgttcgg
     1921 agccgcggag gtgcttactg gcctggagat ggacagctac gcgccgtttc catttctgcc
     1981 accggtcgtg cctggaaaat acggcaaggt ctacgaattc cggacctatc acctcaagcc
     2041 cggtggcttg acgccgacga ttgcagcctg ggaggctgcg atgcccgagc gcgcgaaact
     2101 ctcgccactt gtgatcaata tgtacgcgct ggacggagcg ccgcgcatca cccacatctg
     2161 gccctatgcc agtctcgacg cgcgagcggc gatccgggcg gactcttacg cccagggaat
     2221 ctggccgccg aagaatgcgc cggagcagtt ctaccacgcg acctcaacaa tcggtctgcc
     2281 cactgcagcc tcgccgctgg cgtgatgagc acgccgcgag cgcctcgggc ttactcgtag
     2341 acgaatgcgc gatcgccaag gtcgatggcc ggaaactcgt ccttttcggc ccagtaatcc
     2401 tggttgtgct gccactccgg cttgtcgccg cgcttcggca acagatgcat gccgcgcatc
     2461 atgtagccgg ggttaaaatt ttcaggatcg atccacggca acagcggcat attgtggtct
     2521 tccgcacgca aagcgggcgt gaccttcttt gcccctgttt ccttcatgtg gttgagcagc
     2581 cgacagacaa aatcggcgac cagatcgacc cgcagcgtcc agctcgcccg gaaatatccg
     2641 aacacccaga ccagattggg cacgccggtg aacatcatgc cgcgataggt cacggtgtcg
     2701 gcgaaatcca gcggcttgcc gtcgatgcaa aattccatgt cgccgttggc actcaggttg
     2761 aagccggtcg cggtgacgat gatgtcggct tcgagcgtct tgccggactt cagcaggatg
     2821 ccggtctcgg tgaagcggtc gatctcgtcg gtcacgaccg aggccttgcc ggagttgatg
     2881 ccctggaaca gatcgccgtc cggaatgaag gcgatgcgct gccgccacgg ccggtatttt
     2941 ggcgtgaagt gggtcgcgat atcgtaatcg gggccgagat aggcttcgac ggcggacagc
     3001 agatccttct tggccgcttc cggttcttcg aaggtcttgc gggtgaacac ggcctgatcg
     3061 tagagaattt tccggcgcgt gatttcatgg atccaggtct cgtcgacctg cagcgcgcgc
     3121 agggtctcgg cgatgtcgat cgcgttgcgt ccggtccgaa aataggtcgg cgaacgctgc
     3181 agcat
//
LOCUS       DASWGM010000978         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_526640,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000978 DASWGM010000000
VERSION     DASWGM010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526640"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VGA15_26350"
     CDS             <1..262
                     /locus_tag="VGA15_26350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591252.1"
                     /translation="GFGNGQQLRNEPDAMTALNAPITYINYRGEILDPYTGSPLPGVD
                     PGALARRSAPFLSRGARVGSQDDQAQQFVTDMVGFFQRQLAP"
     gene            complement(324..914)
                     /locus_tag="VGA15_26355"
     CDS             complement(324..914)
                     /locus_tag="VGA15_26355"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9591253.1"
                     /translation="MRAGEKLFRAQGYSGTGLKQLAAEAQAPWSSMYHFFPNGKEQLA
                     EEVIRYGGDLYEQMIRQCFAAYPDPLEAVGAMFTGEAKILRSSGFRNGCPVASVALDV
                     ASTTDRVRKPCADVFASWIRALAEGIAASGIPEEMATDLASYILASLEGAIILSRTSR
                     SVRPIEQTAQFVLDTLEDKLPRRSRKAKNARQNVKS"
     gene            1131..1613
                     /locus_tag="VGA15_26360"
     CDS             1131..1613
                     /locus_tag="VGA15_26360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591254.1"
                     /translation="MMHRRALLAALCGGLVAGTLDIGVAALLNSVSPLVVLVFIASGI
                     LGKAAYHTGAAGLVLGLALQWAMSFAIALIYGLGRERMEVLRHSWILGGALYGLAIFL
                     VMSFAVVPLSRAVPQPVFTLPAFVENLLAMILFGLIVAFCAHRFGAGPQRSLTLTSAG
                     "
     gene            complement(1655..2578)
                     /locus_tag="VGA15_26365"
     CDS             complement(1655..2578)
                     /locus_tag="VGA15_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX9591255.1"
                     /translation="MSRKVIITCAVTGAIHTPSMSKALPVTPQEIADAAVDAAEAGAA
                     IVHLHARNPKTGQPDQSPEAFAPFLKIIKQRSNCVINLTTGGAPTMTVDERVRPAAVY
                     KPEVASLNMGSMNFAFFGMLNRFKKFDHDWEQKHLQNKDIVFRNTFQDIEYVLKTLSE
                     TGTRFEFECYDTAHLYNLNYFLEQGMVKPPLFVQTVFGLQGGTGAHPEDVLHMKRTAD
                     RLFGDKMVWSVLGAGRNQLPIAAMAAAMGGNIRVGLEDSLWAAPGRMAASNAEQVTLA
                     RKIIEGLGLQVATSDEAREILDLKGGDKLEV"
     gene            complement(2624..3568)
                     /locus_tag="VGA15_26370"
     CDS             complement(2624..3568)
                     /locus_tag="VGA15_26370"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX9591256.1"
                     /translation="MASPKRHVAIVGTGLIGRAWAAIFARAGWKVRLSDPHIPTLKAA
                     PRLIRDELHALARHGLTGDPDGAVARISVAGSLREAVMDVEFVQENGPEKVEDKRAIF
                     AQLDRLAPPDALLVSSTSAITASRFTETLPGRARCLVGHPVNPPHLVPLVELCGAPWT
                     SADAIDRARTIYREIGQVPVTINREINGFVLNRLQGALLAEAFRLVGEGFISAEDLDH
                     TVKDGLGLRWSFLGPFETIELNAPGGIPDYCARYTGFYKELASAAAGPEVYQSPNVDR
                     VIAAWPHQPTSERLAALTQRRNDRLAALAAHKARPNDK"
     gene            complement(3590..>5293)
                     /locus_tag="VGA15_26375"
     CDS             complement(3590..>5293)
                     /locus_tag="VGA15_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9591257.1"
                     /translation="EFLGIYTENWLFWFGLVFVCFIVFSPTGLIGVGERLIAPFRKKT
                     TEDAAMSARRIEVLPLPQFLRPKSTVVGPVLSARHIVKSFGGIKAVQGIDISIIDRTL
                     HALIGPNGAGKTTAFNLLSGMYPPDQGSVSLMGQPIAGHTPEEIARAGIGRSFQITNL
                     FPSLSVAENIRLAVQARHPRRFDPLTNALSIHAINAETDATIRYLGLAGIEKAEAGML
                     SYGGQRLLDMGVALATAPRILLLDEPLAGLAAAERERIGAIIKRISSDLPVLLVEHDI
                     DRVFQLADRVTVMNEGRVLLDGLVEDARASPKVQEVYIGSGATEVAARPRETAARVNA
                     LLTATHVDTFYGKSHILNDVNFSLHENEIIALLGRNGAGKSTLLKTLIGIAPASNGSI
                     RLADSELIGLSSAQTARLGIGYVPQGRGLFAGMSVEQNLELGALRRQTGNGVHWTRQR
                     IYEYFPRIRERLDSPADYLSGGEQQMVAVARALSGDVRVLLLDEPFEGLAPTVVEQLF
                     ETFDRLRKEIAIIIVDHNLDLALALSDTTVALERGQVIHQGPSKALRDDLDLRRKVLW
                     L"
BASE COUNT          957 a         1608 c         1660 g         1068 t
ORIGIN      
        1 cgggttcgga aacggtcagc agctacgaaa cgagcccgac gccatgacgg cgctcaacgc
       61 acccattacc tatatcaact atcgaggcga gatcctggat ccatataccg gatcgccgct
      121 gccgggcgtt gacccgggcg ctctggcaag gcgaagcgcg ccattcctgt cgcgcggtgc
      181 cagagtgggt agtcaggacg atcaggctca acaattcgtc acggatatgg tcggcttttt
      241 ccagcggcag cttgcgccct aggaagcttc cgggaagctc cacctggcgc ggcgagctaa
      301 agcactgccc ggcaaattgg gggttacgat ttcacgtttt ggcgcgcgtt ctttgccttg
      361 cgcgaacggc ggggcaattt gtcttcgagt gtgtccaata cgaactgggc ggtctgttca
      421 ataggcctga cgctcctgga cgtccggctc aagatgatag ctccttccag ggaggccagg
      481 atatagcttg ccagatccgt cgccatttcc tccggtattc cggacgcggc tattccctcg
      541 gccaacgcac gtatccacga tgcaaaaacg tcggcgcacg gcttgcggac cctatcggtc
      601 gtgctggcga cgtcgagcgc aactgatgca accggacatc cgtttcgaaa ccccgatgac
      661 cgaagaatct tggcctcgcc ggtgaacatc gcgccgacgg cctcaagagg gtcaggatat
      721 gccgcgaaac attgccggat catctgttca tacaaatcgc ctccataccg gataacctct
      781 tcggcgagtt gctcctttcc gttcgggaag aagtgataca tcgaactcca gggcgcctgg
      841 gcttctgccg ccagctgctt gaggccggtg ccggaatagc cctgagctcg aaacagcttt
      901 tcgccggcgc gcaataatcg atccttggtc tcagacatcg agtccgttcc tttttgcagc
      961 cggtcaacac atcgcaggtc ttgcggcgca tcacagatct atcacaggga tcgccaaaca
     1021 aaagtctgtt ttggcgctga atgtattgat ctatacaatc tagcagggcg cggctgcggc
     1081 ggccggtatt ggtgaaacgg tccccggcct ctcatttatc gcaggagatg atgatgcatc
     1141 gtcgcgcttt actggccgcc ttgtgcgggg gtttggtggc cgggacgctc gatatcggcg
     1201 tggcggcgct tttgaattca gtcagtccgc tggtcgtgct tgttttcatc gcgagcggaa
     1261 ttttgggcaa ggccgcctat cacacagggg cggccggatt ggtattgggg ctcgcactgc
     1321 agtgggcgat gtcgttcgcg atcgcattga tttatgggct tggacgcgag cggatggaag
     1381 tgctgaggca ttcgtggatc ttaggcgggg ctctgtacgg ccttgcgatc ttccttgtca
     1441 tgagctttgc cgtcgtcccg ctgtcgagag ccgtgccgca acctgtgttt acgcttccag
     1501 cgttcgtgga aaatcttctg gcaatgatcc tgtttggctt gattgttgcc ttctgcgcgc
     1561 atcggttcgg cgcggggccg caacgttcat taactttgac atccgccgga taacgggaga
     1621 tggcggtccg ggtgcgacgg gccgccgttc accgtcacac ttccagcttg tcgccgccct
     1681 tcagatcgag aatttcgcgc gcctcgtccg atgtcgctac ctggaggcca aggccttcga
     1741 tgatcttgcg ggcgagcgtt acctgctcgg cattcgacgc cgccatccgg ccgggcgccg
     1801 cccacagcga atcttcaagc ccgacgcgga tattgccgcc catcgccgct gccatcgccg
     1861 cgatggggag ttgattgcgc ccggcgccga gcaccgacca gaccatttta tcgccgaaca
     1921 agcgatcggc ggtgcgcttc atgtgcagca cgtcctccgg atgggcgccg gtgccgccct
     1981 gcaggccgaa caccgtttgc acgaacagcg gcggcttcac catgccctgc tcgaggaagt
     2041 agttcagatt gtagaggtgc gcggtgtcgt agcattcgaa ctcgaagcgc gtgccggtct
     2101 ccgacagggt cttcaagacg tattcgatgt cctggaaggt gttgcggaac acgatgtcct
     2161 tgttctgaag atgcttttgc tcccaatcat ggtcgaactt cttgaatcga ttcagcatgc
     2221 cgaaaaaggc aaaattcatc gagcccatgt tgagggaggc gacttccggc ttgtagaccg
     2281 cggcggggcg gacccgctcg tcgaccgtca tggtcggcgc gccgcccgtg gtgagattga
     2341 tcacgcagtt ggagcgctgc ttgatgatct tcaggaacgg cgcaaaggct tcgggactct
     2401 ggtcgggttg accggtcttc gggttgcggg catgcagatg cacgatggca gcacctgcct
     2461 ccgccgcatc gaccgccgca tcggcgattt cctgcggtgt caccggcagg gccttcgaca
     2521 tcgagggggt gtggatcgcg ccggtcacgg cgcaggtgat gatgactttg cggctcatgt
     2581 tttgctctcc tttttgtagg gcgaaccttc aggatttgtc gttttacttg tcgttcggcc
     2641 tggccttgtg cgcggccagc gccgcgagac ggtcgttgcg ccgctgcgtc agcgcagcta
     2701 aacgttcgga cgtaggctga tgcggccacg ccgcgatcac ccgatccaca ttggggcttt
     2761 ggtaaacctc gggcccggcg gctgccgacg caagttcttt gtaaaagccc gtatagcgcg
     2821 cgcaatagtc gggaatgcca ccgggcgcat tgagctcgat ggtctcgaac ggtccgagaa
     2881 acgaccagcg cagtccgagg ccgtccttca cggtgtgatc gagatcctcc gccgaaataa
     2941 aaccttcgcc gaccagacgg aaggcttcgg ccaatagcgc gccctgcagc cggttcagca
     3001 caaaaccgtt gatctcgcgg ttgatggtga ccggcacctg cccaatctcg cgatagattg
     3061 ttcgcgcacg gtcgattgca tcagccgacg tccacggcgc gccgcagagt tcgaccagcg
     3121 gcaccagatg cggcggattg acgggatggc cgaccagaca gcgggcgcgg ccggggagcg
     3181 tctccgtgaa ccgcgaggcg gtgatggccg aggtcgagga gaccagcagc gcgtccggcg
     3241 gcgccagcct gtcgagctgg gcaaagatcg cccgcttgtc ttcgaccttc tccggtccgt
     3301 tttcctggac gaattcgacg tccatcaccg cctctctgag gctccccgca acggagatcc
     3361 gtgccacagc gccatcggga tcgccggtca atccgtggcg cgccagcgcg tgcaattcat
     3421 cgcggatcag gcggggcgcc gcctttagcg tcgggatatg cggatcgctc agccgcacct
     3481 tccatccggc acgggcaaag atcgcggccc aggcgcgtcc gatcaggccg gtgccgacaa
     3541 tggcgacgtg tcgtttgggg cttgccattt tcgtttctcg cattcgttct cacagccaga
     3601 gcaccttgcg gcgcagatcg agatcgtcgc gcagtgcctt ggacggtccc tggtgaatca
     3661 cctggccgcg ctccagcgcc accgtggtgt ccgacaacgc cagcgccaga tcgagattgt
     3721 gatcgacgat gatgatggcg atttccttgc gcaggcgatc gaaggtttcg aacaattgtt
     3781 cgaccacggt cggcgccaga ccctcgaacg gctcatcgag cagcaatacc cgcacgtcgc
     3841 ccgacagcgc gcgggcgacc gccaccatct gctgctcgcc gccggaaaga taatccgcag
     3901 gactatccag ccgttcgcga atccgcggga aatattcgta gatgcgttgc cttgtccagt
     3961 gcacgccatt gccggtctgg cgcctcagcg cgccgagctc aaggttctgt tcgacgctca
     4021 tgccggcgaa caggccgcgg ccctgcggca cataaccgat gccgagccgc gcggtctgcg
     4081 ccgacgaaag tccgatcaac tcgctgtctg caagccggat cgatccgttc gacgctggag
     4141 cgatcccgat cagggttttg agcagcgtcg acttgccggc gccgttgcgg ccgagcagcg
     4201 cgatgatctc gttttcatgt aggctgaagt tcacatcgtt gagaatgtgg ctcttgccgt
     4261 agaaggtatc gacatgggtt gccgtcaata gcgcgttgac gcgggcggcg gtttcgcgcg
     4321 gacgcgcggc aacctcggtc gcgccggagc cgatataaac ttcctgcacc ttcgggctgg
     4381 cgcgcgcgtc ttccaccaaa ccgtcgagca gcacccggcc ttcattcatc accgtgacac
     4441 gatcggcgag ctgaaacacc cggtcgatgt cgtgttcgac cagcagcacc ggcaaatcgg
     4501 acgagatgcg cttgatgatg gcgccgatgc gctcgcgttc cgcggcggca agcccggcga
     4561 gcggctcgtc aagcaagaga atccgcgggg ccgtggcgag cgccacgccc atgtcgagca
     4621 ggcgctggcc gccatacgac aacatgccgg cttcggcctt ttcgatgccg gcaaggccga
     4681 gatagcggat ggtcgcatcg gtttcggcat tgatcgcatg gatcgacagc gcattggtga
     4741 gggggtcgaa gcggcgggga tgccgcgcct gcacggcgag gcggatgttt tcggccacgc
     4801 tcagcgatgg aaacagattg gtgatctgga acgagcggcc gatgccggcg cgcgcgatct
     4861 cctccggcgt atgtccggcg atcggctggc ccatcagcga aaccgaaccc tggtccggcg
     4921 gatacatgcc cgacaaaaga ttgaacgccg tggtcttgcc ggcgccgttg ggcccgatca
     4981 gcgcgtgcag cgtgcggtcg atgatggaga tgtcgatgcc ctgcaccgcc ttgatgccgc
     5041 cgaaactctt gacgatgtgg cgggcggaaa gaaccggtcc gacgacggtg gatttcggcc
     5101 gcaagaattg cggcagcggc agcacttcga tccggcgcgc cgacatcgcg gcatcttctg
     5161 ttgtcttctt gcggaagggt gcgatcagcc gttcaccgac accgatcagg ccggtcggcg
     5221 aaaacacgat gaagcaaacg aacaccagtc cgaaccagaa cagccagttc tcggtataga
     5281 tgcccagaaa ttc
//
LOCUS       DASWGM010000979         7179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1083182,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000979 DASWGM010000000
VERSION     DASWGM010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083182"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VGA15_26380"
     CDS             <1..1053
                     /locus_tag="VGA15_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018117166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX9591258.1"
                     /translation="VYEKISRRFHENPDQFADAFAKAWYKLTHRDMGPIVRYLGPLVP
                     KEELPWQDPIPAVDHELIGEQDIAALKAKILASGLSVSQLVSTAWASASTFRGSDKRG
                     GANGARIRLAPQKDWDVNQPAQLAKVLAKLEAIQKEFNASQSGGKKVSLADLIVLGGS
                     AAIEKAAKDAGHDVKVPFTPGCMDASQEQTDVESFAPLELTADGFRNYLRGKQRLSPE
                     EQLVDRAQLLTLTAPEMTVLVGGLRALNAGQPAHGVLTKRPETLTNDFFVNLLDMSTH
                     WQPSAGTEGVFEGRDRKTNQVKWTGTRVDLIFGSHSQLRALAEVYACADSKEKFVKDF
                     VAAWTKVMNLDRFDLA"
     gene            complement(1125..1796)
                     /locus_tag="VGA15_26385"
     CDS             complement(1125..1796)
                     /locus_tag="VGA15_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX9591259.1"
                     /translation="MMKECGAEFVGTFMLVSSVCGAALFSAPSVGLIAVALSIGTAVL
                     AMAYALGPISGGHFNPAVTCGLVAAGRFEAGKAIPYIIAQVIGGVVAAAVFYVILQGA
                     PASGKWNTFLAVSNTYGGTGFPLLSVGLIEIVMTALFLVVITGATSAKASPGFAPIAI
                     GLTLVTIHLVAIPVSNASVNPARSTATAIFGGAEAMQSLWLFWVAPIVGGIIGGLIGK
                     AFYER"
     gene            2068..2526
                     /locus_tag="VGA15_26390"
     CDS             2068..2526
                     /locus_tag="VGA15_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX9591260.1"
                     /translation="MPTIRPARADEYDEIARVWMNSWASTGLEDASEPLLAKLRARIR
                     LEVEKGWSLYVADDRGTLAAMLALHLPQLYLDQLFVAPEYQGQNLGGQLLAFTRRQLP
                     DEIWLRCARGNEKAWRWYEREGFVFEKKAVEPMTGRVMKYYRWKRGGTGG"
     gene            2634..3476
                     /locus_tag="VGA15_26395"
     CDS             2634..3476
                     /locus_tag="VGA15_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591261.1"
                     /translation="MVRSFAVAAVLSCLVAPAWAQAQPPTGTAATPSVVPAAKPAVKK
                     PAQKTKTTVKPPAPTDSGPCLLGVIPAIGDQFVVQKVGLTIFGNEYTEVPIDAWGLDD
                     LVVARVRAAVAPGTGVRRIAYPKAAFAPYDHPAPALFRSSRDDLTAIVRQITANAGCE
                     RYVVVTKFTGQIDGTNQTHRGIGVLNRGTSLLSHTSLFAAVEVTVFDGQTFAIHKKPF
                     SLGSVLAGAFAHMTQDPLTELDNAAFPELATDTAVSATLRDRTRALLTANLDKTLPVI
                     LKEE"
     gene            3483..4079
                     /locus_tag="VGA15_26400"
     CDS             3483..4079
                     /locus_tag="VGA15_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9591262.1"
                     /translation="MMKLYWSPRSRSFSALWLMEETGKPYERVLTDISTGAQKTPEYL
                     AINPMGKVPALTDGDATLAEAAAICAYVAERYPEAKLAPPPGDPLRAKYLYWLFFAPG
                     CVEPAMVQVATKIEMNPVAAGWGDAQRVFDVLDVALAKGPWILGEQFSAADIIIGSGL
                     NFAVRLFKMVPSRPSFDRYIDRCAARPAFQRASALAAG"
     gene            complement(4253..5065)
                     /gene="fabI"
                     /locus_tag="VGA15_26405"
     CDS             complement(4253..5065)
                     /gene="fabI"
                     /locus_tag="VGA15_26405"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX9591263.1"
                     /translation="MQDLMKGKRGLIMGVANDHSIAWGIAKALAAQGAELAFTYQGEA
                     LGKRVKPLAEQLGVPTVLPCDVEDIASVDAVFDALRARWGQLDFLVHAIGFSDKNELK
                     GRYADTSRENFSRTLVISCFSFTELAKRAAAMMPERGGSMITLTYGGSTRVLPNYNVM
                     GVAKAALEASVRYLAADFGPRNIRVNTISAGPIRTLAGAGIADQRDMLRFQQLHAPLR
                     RTVTIDEVGGSALYLLSDLSTGVTGEIHYVDSGYNIISMPRPEDLKNGNGAE"
     gene            complement(5201..6424)
                     /gene="fabB"
                     /locus_tag="VGA15_26410"
     CDS             complement(5201..6424)
                     /gene="fabB"
                     /locus_tag="VGA15_26410"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEX9591264.1"
                     /translation="MRRVVITGMGIVSSIGNNTQEVLASLHEAKSGITRAEKYAELGF
                     RSQVQGAPTLDPAGVIDRRAMRFLGEGAAWNHVAMEQAIQDSGLEPADVSNNRTGIIM
                     GSGGPSARTIVEAADITRTKGPKRVGPFAVPKAMSSTASATLATWFKIKGVNYSISSA
                     CATSNHCIGNAYETIQIGKQDIIFAGGCEELDWTLSVLFDAMGAMSSKYNDTPATASR
                     PYDVSRDGFVIAGGAGVVVLEELEHAQARGARIYGEIVGYGATSDGYDMVAPSGEGAE
                     RCMKMAMATVNTRIDYINPHATSTPAGDPPEIEAIRKVFGTGDKCPPISATKALTGHS
                     LGATGVQEAIYALLMMNNGFICESAHITELDPAFADMPIVRKRIDNAKLGTVLSNSFG
                     FGGTNATLVFRRMDA"
     gene            complement(6531..7034)
                     /gene="fabA"
                     /locus_tag="VGA15_26415"
     CDS             complement(6531..7034)
                     /gene="fabA"
                     /locus_tag="VGA15_26415"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEX9591265.1"
                     /translation="MLDRRGGYEYEDLLACGRGEMFGPGNAQLPLPPMLMFDRISEIS
                     KTGGEYGKGVIRAELDVNPDLWFFGCHFKGDPVMPGCLGLDALWQMVGFFLGWTGGEG
                     RGRALGLGELKLSGQVMPNVRKIVYNVDIKRVIRLKLVLGVADGWLSADDEIIYRAKD
                     LKVGLFK"
BASE COUNT         1414 a         2276 c         2149 g         1340 t
ORIGIN      
        1 gtttacgaaa agatttcgag gcgcttccac gagaatccgg atcagttcgc ggatgcgttc
       61 gccaaggctt ggtacaagct cacgcaccgc gacatgggcc cgatcgtgcg ttatctcggc
      121 ccgctcgttc cgaaggagga actgccgtgg caagacccca tccccgcagt cgatcatgaa
      181 ctgatcggcg agcaggacat tgccgctctg aaggccaaga tcctcgcgtc tgggctgtcc
      241 gtgtcccaat tggtttcgac ggcttgggcc tctgcctcga cgttccgcgg ctccgacaag
      301 cgcggtggcg cgaacggggc gcgcattcgc ctcgcgccgc agaaggattg ggacgtcaac
      361 cagccggctc agctggcgaa ggtccttgcg aaactcgaag cgatccaaaa ggaattcaac
      421 gcttcgcagt ccggcggcaa gaaggtctcg ctcgctgacc tgatcgtcct cggcggcagc
      481 gccgcgatcg agaaagccgc gaaggacgcc gggcacgatg tgaaggtgcc cttcacgccg
      541 ggctgtatgg acgcgtcgca ggagcaaacc gacgtcgaat cctttgctcc gctcgaactg
      601 accgctgacg gtttccgcaa ctatctccgt ggcaagcagc gcttgtcccc cgaggagcag
      661 ttggtggatc gggcgcaatt gctgacgctg acggcgcccg agatgacggt gctcgtcggc
      721 ggcctgcgcg cgctgaacgc cggacaaccg gcgcacggcg tcctcaccaa acggcccgag
      781 acgctgacga acgacttctt cgtcaatctg ctcgacatga gcacgcactg gcagccgtcc
      841 gctggaaccg aaggcgtgtt cgaagggcgc gaccggaaga cgaaccaggt caagtggacc
      901 ggcacccgtg tcgacctgat cttcggttcg cactcccagc tgcgggccct cgcggaagtc
      961 tatgcgtgcg cggactcgaa ggagaagttt gtgaaagact tcgtggcggc gtggaccaag
     1021 gtgatgaacc tggaccgctt cgacctcgcc tgatcgccgc ggaaagagcc ctcgcgataa
     1081 aaaatcgcga gggctctttc gttgcgcgaa gccagccttc gaatttacct ttcgtaaaac
     1141 gccttgccga tcaggccacc gatgatgccg ccgacgatcg gcgcgaccca aaacagccac
     1201 agcgattgca tcgcctccgc gccgccgaaa atcgcagtgg cggtcgaacg tgccggattg
     1261 accgaggcat tcgaaaccgg aatggcgacg agatgaatcg tcaccagcgt caatccgatg
     1321 gcaatcggtg caaagccagg cgatgccttc gccgaggtcg cgcctgttat caccaccaga
     1381 aacagcgcgg tcatgacaat ttcgatcaga ccgacactca ataacggaaa gcccgtaccg
     1441 ccataggtgt tggacaccgc gaggaacgta ttccattttc ccgatgcggg cgcaccctgc
     1501 aggatgacgt agaataccgc cgctgcgaca acaccgccga tcacttgcgc gatgatgtag
     1561 gggatcgcct tgcccgcctc aaagcggccc gccgccacca gcccacacgt cacggcagga
     1621 ttgaaatgac cgccggagat cggacctagc gcataggcca tcgccagcac ggcggtaccg
     1681 atcgaaagcg cgacggcgat caaaccgacg ctcggggcgg aaaaaagagc cgctccacaa
     1741 acggacgaca ccagcatgaa tgtaccaaca aattctgctc cgcactcctt catcatatcg
     1801 aaatcctccc cttcacccgg cttatgctcc ggctcgtgct tcggacggtg catcatgaca
     1861 tcaagctgtc accgatgaca acttgcgcgc gaattccaca cctcgcctgg atgcggaatg
     1921 ctgcgccgca ttctgttgaa aggcgtgatg accccccccg aaattccata cccgccaagc
     1981 aattgatatc ccgccgattt gccgtatcgt cgcagacaat cttgggcgac ctggcagaca
     2041 ggttgtccac cagggatcgt ctcgatgatg ccaaccatcc gtcccgcgcg cgccgacgaa
     2101 tatgacgaga tcgcccgcgt ctggatgaat agctgggcct cgaccgggct cgaggatgcc
     2161 agtgagccgc tgctggcgaa gctgcgcgcg cgcatccggc tggaggtgga gaaaggctgg
     2221 agcctctacg tcgccgacga tcgcggaacg ctggcggcga tgctggcgtt gcatctgccc
     2281 cagctttatc tcgaccagtt gttcgtggca cccgaatatc agggccaaaa cctcggcggg
     2341 cagctgctcg ctttcactcg gcggcaattg ccggatgaaa tctggctgcg ctgcgcgcgc
     2401 ggaaacgaga aggcgtggcg ctggtacgag cgtgagggtt ttgtgtttga aaagaaagcg
     2461 gtcgaaccga tgacggggcg tgtcatgaaa tactatcggt ggaagcgagg cggaaccggc
     2521 ggatgaagaa tcttgcggac gccttggaca cgctctagtt ttggattccc gcaacaaccc
     2581 gtcttgcctt cgccgaactg tttcggcgat tcctgttttt cccggagttt ctcatggttc
     2641 ggtcgttcgc cgtcgcggct gtcctgtcat gcctcgtcgc gccggcttgg gcgcaggccc
     2701 agcccccaac agggacggcg gcgacaccct cggtagtgcc tgccgcgaag ccggcggtta
     2761 aaaagccggc gcagaaaacc aagaccaccg tcaagccgcc tgcgcccacg gacagcggac
     2821 cttgcctcct cggtgtcatt ccggcgatcg gcgaccagtt cgtggtccag aaagtcggcc
     2881 tcaccatctt cggcaacgaa tatacggaag tgccgatcga tgcctggggc ctcgacgatc
     2941 tcgtggtcgc gcgggttcgt gcggcggttg ctcccggcac cggggtaaga agaatcgctt
     3001 atcccaaagc ggcgtttgcg ccttacgacc atcccgcccc cgcgctgttt cgtagctctc
     3061 gggacgactt gaccgccatc gttcggcaaa tcacggccaa cgccggttgt gagcgctatg
     3121 tagtcgttac caagtttacc gggcagatag atggtaccaa tcaaacccac cgcggcatcg
     3181 gcgtgctgaa ccgcggcacc agccttctga gtcacacctc tttgttcgcc gctgtagagg
     3241 tgacggtatt tgacgggcaa accttcgcca tccacaaaaa gccttttagt cttggatccg
     3301 tattggccgg agcttttgcc cacatgaccc aggacccgct taccgaactc gacaatgccg
     3361 catttccgga gctcgcaact gacacagccg tcagcgcgac gttgcgcgat cgtacgcggg
     3421 ccttgcttac ggcaaacctc gacaagacat tacctgtgat tctcaaggag gagtaggtca
     3481 cgatgatgaa actctattgg tcgccacgct cgcggtcgtt ctccgcattg tggctgatgg
     3541 aagaaaccgg aaaaccttac gagcgcgtgc tgaccgacat ttccaccggc gcgcagaaga
     3601 cgccggaata tcttgcaatc aatccgatgg gcaaggtgcc ggcgctgacg gacggcgatg
     3661 cgacgctcgc ggaagccgcc gcgatctgcg cctatgtcgc cgaacgctat ccggaagcga
     3721 aactcgcccc gccgccgggc gatccgctgc gcgcgaaata tctttactgg ctgtttttcg
     3781 cacccggctg cgtcgaaccc gcgatggtgc aggtcgcgac caagatcgag atgaatccgg
     3841 tggcggcggg ctggggcgat gcccagcggg tattcgacgt gctcgacgta gcgctagcga
     3901 agggaccgtg gatcctcggc gaacaattct cggccgcgga tatcatcatc ggctcggggc
     3961 tgaacttcgc ggtgcggctg ttcaagatgg tgccgtcgcg cccatcgttc gaccgttaca
     4021 tcgatcgctg cgcggcgcgg ccggcattcc agcgcgcgag cgcgctcgcg gcgggataga
     4081 gggggcatcg tgttcccaca cccacaactg tcatcgcccg gcttgaccgg gcgacccagt
     4141 attcgagagc gccagtgctt gagtcgaatc gccggcgtac tggatccccg ctttcgcggg
     4201 gatgacggcc gaatgttggg ctgcgccgtg cgcaggcttc gacgtgcgaa aactactccg
     4261 ccccgttccc gtttttcaaa tcttccggcc ggggcatcga aatgatgttg tagccggaat
     4321 cgacataatg gatttccccg gtcaccccgg tcgacagatc cgacaacaga tacagtgccg
     4381 aaccgccgac ctcgtcgatg gtgacggtgc gccgcagcgg cgcgtggagc tgttgaaacc
     4441 tcagcatgtc gcgctgatcg gcgatccccg caccggcgag cgtgcgaatg ggccccgccg
     4501 agatggtgtt gacgcgaata ttgcgcggac cgaaatcggc agcgagatag cgcaccgagg
     4561 cttccagcgc ggccttggcg acgcccatca cattgtagtt cggcagcacg cgggtcgaac
     4621 cgccataggt caaggtgatc atcgagccgc cgcgctcggg catcatggcc gccgcacgct
     4681 tggcaagttc cgtgaaggag aagcatgaga tcaccagcgt ccgcgaaaag ttttcgcgcg
     4741 aggtgtcggc gtagcggccc ttcaactcgt tcttgtcgga aaagccgatg gcatggacca
     4801 gaaaatccag ctgcccccat cttgcacgca acgcatcgaa aacggcatcg acgctggcga
     4861 tgtcctcgac atcgcagggc agaaccgtgg gtaccccaag ctgttcggcg agcggcttga
     4921 cgcgcttgcc caaggcttcg ccctgatagg tgaatgcaag ctcggcgcct tgtgcggcga
     4981 gcgctttggc aatgccccag gcaatcgaat ggtcgttggc aacgcccatg atcaagccgc
     5041 gcttgccctt catcaggtcc tgcatacttg ccgtgctcca ttcatcccta atgatgtcat
     5101 cacccgcgaa agcgggtgat ccggtaagcg ttgcgatgaa acacgagagc cgcggaatac
     5161 tggatgcccc gctttcgcgg ggcatgacga acacaaaatc tcacgcatcc atccgcctga
     5221 acaccagcgt ggcgttggtg ccgccgaacc cgaaggaatt cgacagcacg gttccgagct
     5281 tcgcgttgtc gatgcgcttg cgcacgatcg gcatgtcggc aaacgccgga tcgagctcgg
     5341 tgatatgggc gctctcgcag atgaaaccgt tgttcatcat caatagcgca tagatcgcct
     5401 cctgcacgcc ggtggcaccg agcgaatgcc cggtcagcgc cttggtcgcc gaaatcggcg
     5461 gacacttgtc gccggtcccg aacaccttgc ggatcgcctc gatttccggc gggtcgcccg
     5521 ccggcgtcga ggtcgcatgc ggattgatgt agtcgatcct ggtattcacc gtcgccatcg
     5581 ccattttcat gcagcgctcg gcgccttcgc cggacggcgc gaccatgtcg tagccgtcgg
     5641 aggtcgcgcc gtaaccgacg atttcgccat agatgcgcgc gccgcgtgcc tgggcatgct
     5701 cgagctcttc cagcaccacc acgccggcgc cgccggcgat gacaaaaccg tcgcggctga
     5761 cgtcgtaggg gcgtgaagcg gtcgcgggcg tatcgttata tttcgacgac atcgcgccca
     5821 tggcgtcgaa cagcaccgac agcgtccagt cgagttcttc gcagccgcct gcgaaaatga
     5881 tgtcctgctt gccgatctgg atggtctcgt aggcgttgcc gatgcaatgg ttcgaggtgg
     5941 cgcaggccga cgaaatcgaa tagttcacgc ccttgatctt gaaccaggtg gcgagtgtcg
     6001 cagacgccgt cgatgacatc gccttcggca ccgcgaacgg tccgacccgt ttcggcccct
     6061 tggtgcgggt gatatcggcc gcttcgacga tggtgcgggc cgacgggccg ccggagccca
     6121 tgatgatgcc ggtgcggttg ttggaaacgt ccgcgggctc gaggccggaa tcctggatcg
     6181 cctgctccat cgcgacgtga ttccacgccg cgccttcgcc gaggaagcgc atcgcgcgcc
     6241 gatcgatgac gccggcggga tcgagcgtgg gtgcgccctg cacctgggag cggaaaccga
     6301 gctcggcata tttctccgcg cgcgtaattc cggacttcgc ctcatggagg ctcgcaagca
     6361 cttcctgggt gttgtttccg atggacgaga cgatgcccat ccccgtgatg acaacccgcc
     6421 tcatgatcgc ctcgccctgt cgttgcgttt tgtgtgctgc cgtcgtcaaa ccgaaagccg
     6481 cctcgtcggc cgaagcgcgt atgttacgtt cccgactgca atgcggcttc ctacttaaac
     6541 agaccgactt tcaaatcctt agcgcgatag ataatctcgt catcggcgga aagccacccg
     6601 tcggcgacgc ctaacacgag ctttaaacgg atcacgcgtt tgatgtcgac gttgtacaca
     6661 atcttgcgaa cgttcggcat cacctgtccg gaaagcttca gctcgccgag accgagcgcc
     6721 cggccgcgcc cctcgccgcc ggtccaaccc agaaagaaac cgaccatctg ccagagcgcg
     6781 tctaatccca gacaccccgg catcacggga tcgcccttga aatggcaccc gaagaaccaa
     6841 aggtccggat tcacgtcgag ttcggcacgg atcacgccct tgccgtattc cccgcccgtt
     6901 ttggaaatct cgctgatgcg gtcgaacatc agcatcggag gcagcggcaa ttgtgcgttg
     6961 cctgggccaa acatctcacc gcggccgcac gccagcagat cctcgtattc gtaaccgccg
     7021 cgccgatcca gcatctgctg accctctctc aaaatcccga tggagcattt tcgagcgaaa
     7081 tggacccgtt tcgcctggga agcgcgccag ttatcatgga tcggcacaaa taccacagct
     7141 cggaattgcc ctgcggcaat tggcgctcgc ctttgccga
//
LOCUS       DASWGM010000980         6780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_662297,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000980 DASWGM010000000
VERSION     DASWGM010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662297"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VGA15_26420"
     CDS             <1..832
                     /locus_tag="VGA15_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9591266.1"
                     /translation="ARTIGQSLSKTWGQQPIIENRPGAGGVIASQALAQSAPDGYRLI
                     LVASGHPLNQFFYPKLPYDTFKDFTAITEVAYSPLAIVVARKNDARNLQELLAVAKQK
                     PDSLSYGTSGNGTSAHLAGELLNYMAGVKILAIPYKGGAPALTAVLAGDIPISINPMP
                     EVVGQLDSGLVRVIAVTTATRSKALPDVATVAESGVPGYETSVWWGFLGPAGMAPELV
                     AKINADIRTALKDPAVLAAFDKLGAVPVGSSPQDFEAFMRAEAAKWGPILKQANVRVE
                     "
     gene            975..2003
                     /locus_tag="VGA15_26425"
     CDS             975..2003
                     /locus_tag="VGA15_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9591267.1"
                     /translation="MIIDVHGHYTTAPKALEDWRNRQIAGIKDPSVRPKVSELKISDD
                     ELRESIVNNQLKKMKERGSDLTIFSPRASFMAHHIGDFEVSSAWAAICNELCARVSQL
                     FPDHFIGAAMLPQSPGVDPKTCIPELEKCVKDYGFVGINLNPDPSGGHWTSPPLSDRQ
                     WYPIYEKMVELDIPAMIHVSTSCNACFHTTGAHYLNADTTAFMQCLTSDLFKDFPTLR
                     FLIPHGGGAVPYHWGRFRGLAQELKKPLLKEHLLRNIFLDTCVYHQPGIDLLTKVIPV
                     DNILFASEMIGAVRGIDPETGFPYDDTKRYIEASTILTAQDKHKVYEGNARRVFPRLD
                     AALKAKRK"
     gene            2005..2700
                     /gene="ligK"
                     /locus_tag="VGA15_26430"
     CDS             2005..2700
                     /gene="ligK"
                     /locus_tag="VGA15_26430"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEX9591268.1"
                     /translation="MTGPINNLGIVKRNIVRADTAAVEKLSRFGVATIHEAMGRVGLM
                     HPYMRPIYVGAQACGTAVTVLLQPGDNWMMHVAAEQLQPGDVVVAACTAENTDGFFGD
                     LLATSFRACGAKGLIIDGGVRDVKDLTEMQFPVFSKAISAKGTVKATLGSVNIPVVCA
                     GALVNPGDVVIADADGVVVVPAGIARQVADAAEAREANEGEKRARLAAGVLGLDMYKM
                     RESLAKAGLRYID"
     gene            2706..3629
                     /locus_tag="VGA15_26435"
     CDS             2706..3629
                     /locus_tag="VGA15_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEX9591269.1"
                     /translation="MSSNSNQWHVGLVGYGEVGRILAEDLRKQDIKVSAYDIKLGDDQ
                     AGPLRDHAGAFGVALASSHADLAAQADFIVSAVTASQAVPVAQLCASAVKNGAWFLDF
                     NSASPGAKRRAAALIDGAGGRYVEGAVMTSVPPYRIKVPLLLGGSGARELAPMLMELG
                     FDAKVASEELGVASAVKMCRSVMIKGLEAMVIECFTTARAYGVEDAVLASLAETYPGI
                     NWEKQGAYFFQRVIEHGRRRSEEVREVAETVREIGLSPWSAQGTAERQAWVADLADEG
                     LFGPKGTKEFARSPDWRTEADRILGKLNRGE"
     gene            3641..4564
                     /locus_tag="VGA15_26440"
     CDS             3641..4564
                     /locus_tag="VGA15_26440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX9591270.1"
                     /translation="MEFQKTPGWLDWYAGPSRPRFKPPAGAVDAHCHVFGPGAEFPYA
                     PERKYTPCDASKQQLYALRDHLGFARNVVVQATCHGADNRAMVDALIDSGGTARGVAT
                     VRRSVSDAELNAMHAAGVRGVRFNFVKRLVDFTPQEELIEIAGRIGKLGWHVVIYFEA
                     VDLPELWDFFTSLPTAIIVDHMGRPDVSKPVDGPEFELFVKFMRDHANVWCKVSCPER
                     LSQSGPPALNGEQNAYRDVIPFARRIVETFPDRVLWGTDWPHPNLKNHMPDDGLLVDF
                     IRHIATTPDLQRKLLVDNPMRLYWPEEAAGR"
     gene            complement(4656..5912)
                     /locus_tag="VGA15_26445"
     CDS             complement(4656..5912)
                     /locus_tag="VGA15_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX9591271.1"
                     /translation="MSICGLDFGTSNTTLGTIEGHAPVLAALETGYTTIPSAIFYEVD
                     GAVLIGRKAIEAYVEGTPGRLMRSLKSVLGTSLIEETTRLGRERVSFRNVIGYYLGAV
                     KRRAEQATGRELRDVVHGRPVHFVDNADDADRKAEQTLREIAREVGFDDVTFQFEPIA
                     AALDYERQIATEEVALIADIGGGTSDFSIVRLGPQRHGKADRAADILASDGVRIGGTD
                     FDRQLSLGVVMPLFGFGSAMKRAALEVPSRYFHDLATWSNINRMYEPRVMTELRQVRH
                     EAREPALLDRLVRVVDEQRGHTLAMEVEEAKIALSDARQADIPLEWVAPGLNAAIARP
                     DLVSHTRQLSERIAARVKICLAQARLSASDIDAVFLTGGSVRLAHVRKAITKAVPSAR
                     IVEGDTFGAVGKGLTLEALRRYGPRD"
     gene            6154..>6780
                     /locus_tag="VGA15_26450"
     CDS             6154..>6780
                     /locus_tag="VGA15_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HEX9591272.1"
                     /translation="MANINRQIAEELGVREQQVEATVALLDGGATVPFVARYRKEITG
                     ALDDAQLRNLEERLNYLRELEERRTAILNSVREQGKLDAALEAAILAADSKGRLEDIY
                     LPFKPKRRTKAEIAKEAGLEPLSELLLTQPQNDPQVVAAGFVDAAKQVADAAAALDGA
                     RAILVERFAEDADLIGTLREQVWSNGRVASTVRRGKKTEGEKFKDYFDF"
BASE COUNT         1313 a         2118 c         2166 g         1183 t
ORIGIN      
        1 cgcgcgcacc atcggtcagt cgctatcgaa gacctggggc cagcagccga tcattgaaaa
       61 ccgtcccggc gctggaggcg tgattgcctc ccaggcgttg gcccagtccg cgcccgacgg
      121 ctatagattg atcctcgttg cgagcggcca tccgctgaac caattcttct atccgaaatt
      181 gccctacgac acttttaagg attttaccgc gattaccgag gtggcgtatt cgccgctggc
      241 catcgtcgta gccaggaaga atgacgccag gaatttgcag gagctgctgg cggtcgcgaa
      301 gcaaaaaccc gactcattgt catatggaac ctccggcaat gggacgtcgg cgcacctcgc
      361 cggtgaactg ctcaactata tggccggcgt caagatcctg gccattccat acaagggcgg
      421 agcgccagca ctcacggcgg tgttggccgg agatataccc ataagcatca atccaatgcc
      481 ggaagttgtt ggccagctcg acagcggcct ggttcgcgtg attgcagtga ccaccgccac
      541 ccgatcgaag gcgctgccgg atgtggccac ggtggcggaa tccggtgtgc ccggctacga
      601 gacttcggtg tggtggggat ttctcggacc ggcgggcatg gcgccggaat tggtggcgaa
      661 aatcaacgcc gacattcgga ctgccctgaa ggatcccgcg gttttggcgg cgtttgacaa
      721 gctgggcgcc gtgccggtgg gaagttcgcc acaggacttc gaggcattta tgcgcgcgga
      781 ggccgccaaa tggggcccga tcctcaagca ggccaacgtc agggtcgagt aatgtcagga
      841 tcgagtacgc ggccagatgc ggttttcggt attaggctcg cgcggcgcgc atagaaattg
      901 atagtttgag aaacgcaagg aagccgcgat gcaaatatca atcgcatctg ccgccatccg
      961 agaaggagag ccaagtgatc atcgacgtgc acggccacta caccacggcg ccaaaggcgc
     1021 ttgaggattg gcgcaaccgg cagattgccg gcatcaagga tccctcggtc aggcccaagg
     1081 tcagcgagct gaagatcagc gacgacgaac tgcgcgagag catcgtcaac aaccagctca
     1141 agaagatgaa ggagcgcggc agcgatctta cgatcttcag cccgcgcgcc agcttcatgg
     1201 cccaccacat cggcgacttc gaggtgtcct cggcctgggc cgcgatctgc aacgagctgt
     1261 gcgcgcgtgt cagccagctg tttcccgatc atttcatcgg cgccgcgatg ctgccgcagt
     1321 cgccgggcgt cgatccgaaa acgtgcatcc ccgaactgga gaaatgcgtc aaggattacg
     1381 gcttcgtcgg gatcaacctg aaccccgacc cgtcgggcgg gcactggacc tcgccgccgc
     1441 tatcggaccg gcaatggtac ccgatctacg agaagatggt ggagctcgac atccccgcca
     1501 tgatccacgt ctccaccagc tgcaacgcct gttttcacac cacgggcgcg cattacctga
     1561 acgccgacac caccgccttc atgcaatgcc tgacctcgga cctgttcaag gactttccga
     1621 ccctgaggtt cctgatcccg cacggcggcg gcgcggtgcc ctatcactgg ggccgcttcc
     1681 gcggcctggc gcaggagctg aagaagccgc tgctgaaaga gcacctgctg cgcaatatct
     1741 tcctcgacac ctgcgtttac caccagccgg gcatcgacct tttgaccaag gtgatcccgg
     1801 tcgacaacat cctgttcgcc agcgagatga tcggcgcggt gcgcgggatc gatcctgaaa
     1861 ccggcttccc ctacgacgac accaagcgtt acatcgaggc ctcgacaatc ctcactgccc
     1921 aggacaagca caaggtctac gagggcaatg cgcggcgcgt gttcccgcgc cttgacgcgg
     1981 cgctgaaagc gaagaggaag taacatgact ggcccgatca ataatctcgg catcgtcaag
     2041 cgcaacatcg tccgcgcgga cacggcggcg gtcgagaagc tgtcgcgctt cggcgtcgcc
     2101 accatccatg aggcgatggg ccgcgtcggc ctgatgcatc cctatatgcg gccgatctac
     2161 gtcggcgcgc aggcctgcgg aaccgccgtc accgtgctgc tgcagccggg cgacaactgg
     2221 atgatgcatg tggcagcgga gcagttgcag ccgggcgatg tcgtggtcgc ggcctgcacc
     2281 gccgagaata ccgatggctt cttcggcgat ttgcttgcga cgagcttccg cgcctgcggt
     2341 gcgaagggcc tgatcatcga cggcggggtg cgtgacgtga aggacctgac cgaaatgcag
     2401 ttcccggtgt tttccaaagc gatcagcgcc aagggcacgg tgaaggcaac cctgggctcg
     2461 gtgaacatcc cggtggtctg cgccggcgcg ctggtgaatc cgggggacgt ggtgatcgcc
     2521 gatgcagacg gcgttgtcgt cgttcctgcc gggatcgccc gacaggtggc cgatgcggcg
     2581 gaggcgcgcg aggcgaatga aggcgagaag cgcgccaggc ttgcggccgg tgtgctgggc
     2641 ctcgacatgt acaaaatgcg cgagtcactc gcgaaggccg gcctcagata tatcgattga
     2701 ccgccatgtc gagcaactca aaccaatggc atgtgggact cgtcggctac ggcgaggtcg
     2761 gccgcatcct tgccgaagac ctgcggaagc aggacatcaa ggtcagcgcc tacgacatca
     2821 agctcggcga cgatcaagcc gggcccttgc gggatcatgc gggagcattc ggcgtcgcgc
     2881 tggcttcctc gcacgccgat cttgccgcgc aggccgactt catcgtctcg gccgtcacgg
     2941 cgagccaggc cgtgccggtg gcgcagctct gcgcgtcggc cgtaaaaaac ggcgcgtggt
     3001 tcctggactt caattcggcc tcgccaggcg caaaacggcg tgccgctgcc ctgatcgatg
     3061 gcgctggtgg ccgctatgtc gaaggcgcgg tgatgacatc ggttcctcca taccgcatca
     3121 aggtgccctt gctgctcggc ggcagcggcg cccgggagct tgcaccgatg ctgatggaac
     3181 tcggcttcga cgccaaggtc gcgagcgaag aactcggcgt cgcctccgcg gtcaagatgt
     3241 gccgcagcgt gatgatcaag ggcctggaag cgatggtcat cgagtgtttc acgaccgcgc
     3301 gggcctacgg cgtcgaggac gcggtgctgg cgtcgctggc ggagacttat ccgggcatca
     3361 attgggagaa gcagggcgcc tattttttcc agcgcgtcat cgaacatggc aggcggcgca
     3421 gcgaagaagt gcgcgaggtc gcggaaaccg tgcgcgaaat cggcctctcg ccctggtcgg
     3481 cgcaaggcac cgccgagcgt caggcctggg tcgccgatct cgccgacgag ggcctgttcg
     3541 gtcccaaggg caccaaggaa tttgcccgca gccccgactg gcgcaccgaa gcggaccgaa
     3601 tcctcggcaa gctcaaccgc ggggaatgaa agacgcggcg atggaattcc agaagacccc
     3661 cggctggctg gactggtatg ccggtccatc caggccccga ttcaaaccgc cggccggcgc
     3721 ggtcgacgcc cactgtcatg tgttcggccc gggggcggag tttccatacg cgccggagcg
     3781 caagtatacg ccgtgtgatg cgtcgaagca gcaactgtat gcgctgcgcg atcatctcgg
     3841 ctttgcccgc aatgtcgtgg ttcaggccac ctgccacggc gccgacaacc gcgcgatggt
     3901 cgacgcgctc atcgattccg gcggcacggc gcgcggcgtc gctaccgtca ggcgcagcgt
     3961 cagcgacgcc gaactcaacg cgatgcatgc cgccggcgtg cgcggcgtgc gcttcaactt
     4021 cgtaaagcgt ctggtcgatt tcacgccgca ggaggaattg atcgagattg ccggccgcat
     4081 cggcaaactg ggctggcacg tcgtgatcta ctttgaagca gtggatctgc ccgaattgtg
     4141 ggacttcttc acctcgctgc cgaccgcgat catcgtcgac cacatgggca ggcccgacgt
     4201 cagcaagccg gtcgatggtc ccgagttcga gctgttcgtg aaattcatgc gggatcacgc
     4261 aaacgtctgg tgcaaggtca gttgcccgga acggctgtcg cagtcgggcc cgcccgcgtt
     4321 gaacggcgag cagaacgcct accgcgacgt gatcccattc gccaggcgca tcgtcgagac
     4381 tttccctgat cgtgtactgt ggggcaccga ctggccgcat ccgaatctga agaaccacat
     4441 gccggacgac ggcctgctgg tcgattttat tcggcacatc gcgaccactc cagacctgca
     4501 acgcaaactg ctggtcgaca atccgatgcg gctttactgg cccgaggaag cagccggtcg
     4561 ataaaagtgc cgatcctgac gattcagggc agcgcggaag tcggttgaag caagccgcga
     4621 caaattgcgt tccgcgaaga tcgccgccgc tgtcctcaat ccctcggtcc atagcgtcgc
     4681 agcgcctcca gcgtgaggcc cttgccgacc gccccaaacg tgtcgccttc gacaatccgg
     4741 gccgacggca ccgccttggt gatcgccttg cggacatggg caagccgcac cgaccctccg
     4801 gtcaggaaca ccgcgtcgat gtcggatgcg gacaatcgcg cttgcgcgag gcagatcttg
     4861 acgcgggccg cgatgcgctc ggacagttgc ctagtatggc tgacaagatc ggggcgcgcg
     4921 atggcggcgt tgagccccgg cgcgacccat tccagcggga tgtcggcctg gcgcgcgtcg
     4981 gacagcgcga tcttggcttc ctcgacctcc atcgccagcg tatggccgcg ctgctcgtcg
     5041 accacgcgca ccaaccgatc gagcagggcc ggctcacggg cctcgtgccg aacctgacgg
     5101 agttccgtca tcacgcgggg ttcgtacatg cggttgatgt tcgaccaggt ggcgagatcg
     5161 tggaaataac gcgagggcac ctcgagcgcg gcgcgcttca tggcgctgcc gaagccgaac
     5221 agcggcatga cgacgccgag actgagctga cggtcaaaat cggtgccgcc gatgcgcacg
     5281 ccgtcactag cgagaatatc ggcggcgcga tcggccttgc cgtgacgctg cggcccgagc
     5341 cgcacgatcg aaaaatccga tgtgccgccg ccgatgtcgg cgatcaaggc gacttcctcg
     5401 gttgcgatct ggcgctcata gtccagcgcc gcggcgatcg gctcgaactg gaatgtgacg
     5461 tcatcgaagc cgacctcgcg cgcaatctcc cgcagggttt gttccgcctt gcggtcggcg
     5521 tcatcggcat tgtcgacgaa gtgcaccggc cggccatgca cgacgtcgcg cagttcacgg
     5581 ccggtggcct gctcggcgcg ccgtttcaca gcgccgagat agtagccgat cacattgcga
     5641 aaactgaccc gctcgcggcc gagccgggtg gtctcttcaa tcagcgaggt tccgagcacc
     5701 gacttgaggc tgcgcatcag gcggccgggt gtaccctcga cataggcctc gatggccttg
     5761 cggccgatca gaacggcgcc gtcgacctcg tagaagatcg cgctgggaat cgtggtgtag
     5821 ccggtttcca gcgccgccaa aaccggtgca tggccttcga tggtgccgag cgtcgtgttc
     5881 gacgttccga aatcaagtcc gcagatcgac atgagcgctc cagaaggagc gtccgtctac
     5941 acattaaagc gccatcgatc caccgttatc ttgatacgca atccaagcgg accgtttttt
     6001 tgaagacggg gttacgctat aggcgaatga tgtccatcca cagaacaatt tgtttgatgt
     6061 gccgggcttg aggaatcaat cggcgatggt ccataaccgg tctccccgcg gccgaccaat
     6121 cacccgccgc gactgctttt agaaagatct cgcgtggcaa acatcaatcg acagattgcg
     6181 gaagagctcg gcgttcgcga acagcaggtc gaggcgacgg tcgcgctgct cgacggcggc
     6241 gccacggttc cgttcgtggc gcggtaccgc aaggaaatca ccggcgcgct cgacgacgca
     6301 caattgcgta acctggaaga gcgcctgaac tatcttcgtg agcttgaaga gcgccgcaca
     6361 gctatcctca attcggtccg cgaacagggc aagctcgatg ccgcactgga agcggcgatt
     6421 ctcgccgcag acagcaaagg ccgcctggaa gacatctatc tgccgttcaa gccgaagcgc
     6481 cgcaccaagg ccgagattgc caaggaggcc gggctcgagc cgctgtccga actgctgctg
     6541 acgcagccgc aaaacgatcc gcaagtcgtg gccgcaggct tcgtcgatgc tgcaaagcag
     6601 gtggccgatg cggcggcagc acttgacggg gcgcgcgcaa tcctggtgga gcgtttcgcc
     6661 gaagacgccg acctgatcgg cacgctgcgc gagcaggtgt ggtcgaatgg acgggtggcg
     6721 tccaccgtgc gcaggggtaa gaaaaccgaa ggcgagaagt tcaaggatta tttcgatttc
//
LOCUS       DASWGM010000981         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1083578,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000981 DASWGM010000000
VERSION     DASWGM010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083578"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VGA15_26455"
     CDS             <1..918
                     /locus_tag="VGA15_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX9591273.1"
                     /translation="IHPVLPPHHEFPYTVRVVSEITESNGSSSMASVCGASLALMDAG
                     VPLKRPTAGIAMGLILEGSRFAVLSDILGDEDHLGDMDFKVAGTDHGITSLQMDIKIA
                     GITEEIMKVALGQAKEGRIHILGEMSKALTAARAELGEYAPRIETFKIATDKIREVIG
                     TGGKVIREIVEKTGAKVNIDDDGTVKVASADGESIKAAIKWIKSIASDPELGQIYDGT
                     VVKVMEFGAFVNFFGAKDGLVHISQLASSRVQKTSDVVKEGDKVKVKLLGFDDRGKTR
                     LSMKAVDQVTGEDLEAKQKTEAPREAAGE"
     gene            1243..1380
                     /locus_tag="VGA15_26460"
                     /pseudo
     CDS             1243..1380
                     /locus_tag="VGA15_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
     gene            complement(1576..2514)
                     /locus_tag="VGA15_26465"
     CDS             complement(1576..2514)
                     /locus_tag="VGA15_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9591274.1"
                     /translation="MITLRQLRYLSALARHGHFGRAAEACAITQPALSMQIRDLERTL
                     GVTVVERRPGEVMLTDVGREIARRADDVLTASRDLVDFARHRGGLLTGRLTLGVIPSL
                     APYLLPRILPLLQTRFPELRLELRETQTRQLVEDIKSGALDAAMLALPIGEPDIDTIA
                     LFEDLFLLAVPSSDPRPEATRVAAEDIDQSRLILLEDGHCLRDQALAFCATAARSRNA
                     SAGGTAFGASSLTTVMQMVAGGYGVTLIPQIAADVERRDDRVKFLRLKNPQPGRSIGL
                     AFRRTSPRKADFAALGEVVKESVDVPPAATASERRS"
     gene            2790..>3125
                     /locus_tag="VGA15_26470"
     CDS             2790..>3125
                     /locus_tag="VGA15_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237447.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HEX9591275.1"
                     /translation="MDAKTDESAGKCPVVHTTAARANRDWWPNQLNLQVLHQHSTLSN
                     PMGEAFDYAEEFKKLDIEALNKDLHALMTDSQEWWPADFGHYGPLFIRMAWHSAGTYR
                     IGDGRGGAGA"
BASE COUNT          639 a          980 c          959 g          547 t
ORIGIN      
        1 atccacccgg tgctgccgcc gcaccacgaa ttcccctaca cggtgcgcgt cgtctccgag
       61 atcaccgagt cgaacggatc gtcgtcgatg gcgtcggtct gcggcgcctc gctggcgctg
      121 atggatgccg gcgttccctt gaagcggccg acggcgggca tcgccatggg cctcattctg
      181 gagggatcgc gctttgcggt gctgtccgac attctcggcg acgaggatca tctcggcgac
      241 atggacttca aggtggcagg taccgatcac ggcatcacct cgctgcagat ggacatcaag
      301 atcgccggga taaccgagga gatcatgaag gtcgcgctcg gccaggccaa ggaaggccgc
      361 atccatatcc tgggtgaaat gtcgaaggcc ctgaccgccg cgcgcgccga gctcggcgaa
      421 tacgcgccgc gcatcgagac cttcaagatt gcgaccgaca agatccgcga agtgatcggc
      481 accggcggca aggtgatccg cgaaatcgtc gagaagaccg gcgccaaggt caacatcgac
      541 gacgacggca ccgtgaaggt cgcttccgcc gacggcgaat cgatcaaggc cgcgatcaag
      601 tggatcaagt cgatcgcctc cgatccggag ctcggccaga tctatgacgg caccgtcgtc
      661 aaggtgatgg agttcggcgc gttcgtgaac ttcttcggcg ccaaagacgg tctcgtccac
      721 atcagccagc tcgcctccag ccgcgtgcag aagacctccg acgtcgtcaa ggaaggcgac
      781 aaggtcaagg tcaagctgct cggcttcgac gatcgcggca agacgcggtt gtcgatgaag
      841 gcggtcgacc aggtgaccgg cgaggacctc gaagccaagc agaagacgga agcgccgcgc
      901 gaagccgccg gcgagtaatc tcgcgcgaat tgagaatacc aagggcggct tttcagccgc
      961 ccttcttttt ttgcgcaaat cacttttcga tgacggacgt gtggaattcc accgcagctt
     1021 cgtgacctcg acaatcgaaa ccgtctgacg gtccccgcgt acatactcat gcgttttgct
     1081 ggatgttcgc cgcgcaacgg ttgcggcgcg acgttctacg tcgctgccca agttattgcc
     1141 gtcacctgcg cgtcgaccgg aaaacagagc gcatgcttct ggctctcaac cttcccttca
     1201 acggagaacc cacgatgact tccatgtccc ggcgccaaca acgatgtgtg ggagcacgct
     1261 tattatctca accatcagaa ccgccgcgcc gactatctca aggcttggtg gaacattttg
     1321 aactggaagg ctatcgaaga gcgctacgtt gcggcaaagg ccggaacact gggcctgtga
     1381 gtgctcgtca atttttgaaa gcaaaaaggg ctgcgcttca gtagccccct ttgttgagca
     1441 tcgtgcctct tcaccggcac atgtcgtcac ccgcgaaagc gggtgatcaa gtattacaga
     1501 gtggtcggca atcgaagcgt gaagccgcgg cgtactggat gccccgcttt cgcggggcat
     1561 gacggccgtg ggtcgtcagc tccgtctttc actcgccgtc gcagcgggcg gcacatcaac
     1621 actctctttc accacctcgc cgagcgcggc aaaatccgcc ttgcgcggtg aggtgcggcg
     1681 gaatgccagc ccgatgctgc ggccgggttg cggatttttc aggcgcagga attttacccg
     1741 gtcgtcgcgc cgctcgacat cggcggcgat ctgggggatc agcgtcacgc cataaccgcc
     1801 cgcgaccatc tgcatcacgg tggtcaggct gctggcgcca aacgccgtcc cgccggcgct
     1861 cgcgttgcgg ctccgcgccg cggtcgcgca aaatgccagc gcctgatcgc gcaggcagtg
     1921 gccatcttcg agcaggatca accggctttg gtcgatgtcc tcggcggcga cccgcgttgc
     1981 ctccgggcgc ggatcgcttg agggcaccgc gagcagaaac aggtcttcga acagggcaat
     2041 cgtatcgatg tcgggctcgc cgatcggcaa ggcaagcatc gccgcgtcga gcgcgccgct
     2101 cttgatgtcc tcgacaagtt gcctggtctg ggtttcgcgc agctccagtc gcaattccgg
     2161 aaatcgggtt tgcagcagag gcagaatccg cggcagcaaa tagggcgcca gggagggaat
     2221 caccccgagc gtcagccgcc ctgtcagcag gccgccgcga tggcgggcga agtcgaccag
     2281 atcgcgcgac gccgtgagga catcatcagc ccggcgggcg atttcgcgcc cgacatcggt
     2341 cagcatcacc tcgcccggcc gccgctccac caccgtgacg ccgagcgtcc gctcaaggtc
     2401 gcgaatctgc atcgacagcg cgggctgggt gatggcgcag gcctcggccg cgcggccaaa
     2461 atggccatgc cgggcgagcg ccgagagata acgaagctgt cgaagcgtga tcattccaat
     2521 caaataaact gatcgagagc tcaagacaat gcgactggac ctgatgttgg atatcttcca
     2581 aagtaccgcc cgcgagttcc gcaaaagtga ccccctgttt tgtccggaat tcatccaagc
     2641 cactggaaac acatcgtttt aggaaggaca gagtttttcg acgcgaaccg gatttcactt
     2701 cgcccgaaga tgctctcgac gcctgcacac ccgccggagt tccatacgga acaactcgaa
     2761 gaaattacag aacgcgatcg gaaagaaaca tggacgcaaa gactgacgaa agcgccggca
     2821 agtgcccggt cgtgcacacg actgctgcca gggctaaccg cgactggtgg ccgaaccagc
     2881 tgaaccttca ggtgctccat cagcactcca ccctgtccaa tccgatgggc gaggccttcg
     2941 actacgcgga ggagttcaag aagctcgaca ttgaggcgct gaacaaggac ctccacgcgc
     3001 tgatgacgga ctcgcaggag tggtggccgg ccgactttgg ccactacggg ccgctgttca
     3061 ttcggatggc gtggcacagc gcaggcacgt accgcatcgg cgacggccgc ggcggcgccg
     3121 gagcc
//
LOCUS       DASWGM010000982         5743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_243067,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000982 DASWGM010000000
VERSION     DASWGM010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243067"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..408
                     /locus_tag="VGA15_26475"
     CDS             4..408
                     /locus_tag="VGA15_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX9591276.1"
                     /translation="MLTALLGAAPAKAQAVGGVWLTQAGDAKVRVSKCGGGICGVVVW
                     LRNPIDPATGKPQIDDKNRNPTLAKRPIIGLPLFGGMQPSGPNKWTGQIYNADDGNTY
                     ASSISVTGPDSLRVEGCMGALCGGENWTRAAR"
     gene            complement(434..1582)
                     /locus_tag="VGA15_26480"
     CDS             complement(434..1582)
                     /locus_tag="VGA15_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 domain-containing protein"
                     /protein_id="HEX9591277.1"
                     /translation="MTTPNDTFYSKIPVFRGFSRLMDPALYSPLPDDWSIGVADIVES
                     TKAIAEARYKAVNMAGASVIAAVTNALAGREFPFVFGGDGASFAVSHEDLARAREALA
                     ATANWVKEDLNLAMRVALVPVAAVRAQGFDVRVARFGPSPNLSYAMFSGGGLGWAETA
                     MKRGEFAVPAAPPGTQPDLTGLSCRFEEIPSARGLILSVLVVPARGADSSAFRDLIED
                     IITRVERSPDAGRPVPPGGPPLRWPPAGLEFEARATRGGPLFKRRAAVLVHTLFAYLI
                     MRLDISVGGFVPKTYVQQVVENSDFRKYDDGLRMILDCTPELEAALTLRLAAAASSGI
                     ARYGLHRQDAAMMTCFTPSALRSDHVHFIDGARGGYASAATALKAMAA"
     gene            1869..3815
                     /locus_tag="VGA15_26485"
     CDS             1869..3815
                     /locus_tag="VGA15_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEX9591278.1"
                     /translation="MNKNTAMGATVRANGTGFRTWAPNAKQVYVVTGSALAAHHEPSW
                     RPAPEHGLTPLGDGSWGGFLAGVGDGEPYMFFVEGIGSAGWKRDAYARELSLSPAFPD
                     STCIVRDPQNYSWRDETWQPPKFNDLIIYQLHVGTWWAQDETGRDVRKLRGGTFLDVV
                     EKLEHLQHLGVNAIQLLPIQEFETAFSMGYNGVDYFSPEGAYAVAPEQLGWRLARINR
                     MLERFGKQPLTAPELAHCTNQLKCLVDLCHLHGIAVIFDLVYNHAGGGFDDRSIWFYD
                     RQPSDDQNNSLFFTDHGWAGGQVFAYWNDWVAQFLIDNACFFLTEFRIDGIRYDEVRV
                     IENNGGRPFCQRLTNAVRATNPAAIQIAEYWNEDRASALRPPPRGLGFDAELGDSLRD
                     ALRDLLRQASAGASATLDLSEVANALVVPAAIPEAWRLVQCLENQDLTFASHSDAARV
                     AMLADSLDRTSWYARSRSRTVTGLLLAAPGIPALFMGQELLEDKNWSDNRQTDGLTGW
                     DALDGADTSRRDFLRFVTDMIELRRSQPALRGSGARVPRAEDFERVIVLHRWIEGEGR
                     DVVVIANLAEQPKQAYAIGLPFAGDWSELLNSDVYDNFPNPAPVGNGGHVRAWGEPLD
                     GFSARAAVTLPANGVIVLAPSRAR"
     gene            complement(3836..4876)
                     /locus_tag="VGA15_26490"
     CDS             complement(3836..4876)
                     /locus_tag="VGA15_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX9591279.1"
                     /translation="MLVHFVTDDAAKIPAIRAMLEPRYHVAPQLLGMGDAQVHSNGVL
                     MVDADLRKIDRVEQIRLVLQELSCISEKLFVVHNRVRSMVAQAYALGATAVVSRPKEI
                     IFKLAQIEIAEKAAQSDFAITSPEITESTAAFASMFSAVRAGKQINLSDAERATLEII
                     NSISEKGLAAWLDDVRRYHEGTFQHCLLVTGVAVGFALEIGFSSLDVKRLGIAATLHD
                     IGKARIPLLVLDKPDRLDPGEEEIMRRHPVIGYELLKDIPDITPEVLDGVRHHHEYLD
                     GSGYPDALTAPEISDLVRMLTISDIFAALIESRPYRPAMPRQTAYKILRGMDGKLELA
                     LVKAFRNVALVA"
     gene            complement(5071..>5743)
                     /locus_tag="VGA15_26495"
     CDS             complement(5071..>5743)
                     /locus_tag="VGA15_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846962.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX9591280.1"
                     /translation="AGEKRSLKQYVADLKPNQTEVYFLAGDSIERLKSNPKLEQAAAR
                     GIEVLLLTDPVDAFWTSAPLDFDGKPLKSLSQGDIDFGLIPRIDDSKDESKSDTDEAA
                     TIAVIKAALGDRVADVRASQRLTSSASCLVAGSHGPDRELERLLARQNRGAGTKPILE
                     INMRHPLVAAISGTQDAATDLSFLLLEQAQILDGELPEDPAAFANRLNQLVLRGMTKG
                     SGQAI"
BASE COUNT         1102 a         1782 c         1765 g         1094 t
ORIGIN      
        1 gcgatcttga cggcattgct tggcgcggcg ccggcgaaag cgcaggccgt cggcggtgtc
       61 tggctgaccc aggcgggtga cgccaaagtg cgggtcagca agtgcggcgg tggaatttgc
      121 ggggtcgtgg tctggctgag aaatccgatc gatcccgcga ccggaaaacc gcagatcgac
      181 gacaagaatc gcaaccccac tttggcgaaa cgcccgataa tcggacttcc gctgttcggc
      241 ggcatgcaac cctcagggcc aaacaaatgg accggtcaaa tctataatgc cgacgacggc
      301 aacacctatg ccagcagcat ttcggtgacc ggtccggaca gcttgagagt cgaaggctgc
      361 atgggcgcgc tctgcggcgg cgaaaactgg acccgggccg cgcgctgacg gttcaatttc
      421 gcgtaaccgc agactacgcc gccatcgcct tcagcgccgt cgccgccgag gcgtagccgc
      481 cgcgcgcgcc gtcgataaaa tgcacgtgat cgctgcgcag tgccgagggc gtaaagcagg
      541 tcatcatcgc cgcgtcctgc cggtgcaggc catagcgtgc gatcccgctc gaggccgccg
      601 cagcgaggcg cagcgtgagt gctgcctcca gctcaggcgt gcaatcgagg atcatgcgca
      661 ggccgtcgtc atatttgcgg aagtcggaat tctccaccac ctgctgcaca taggttttcg
      721 gcacgaagcc gccgacgctg atgtcaaggc gcatgatcag ataggcgaac agcgtatgca
      781 cgagcacggc ggcgcgccgc ttgaacagcg gtccgccccg cgtggcccgg gcctcgaatt
      841 cgaggccggc cggcggccat cgcagcggcg ggccgcccgg cggcaccggc cgccccgcat
      901 ccggactgcg ctcgacccgc gtgatgatgt cttcgatcaa gtcacgaaag gcgctggaat
      961 cggcgccgcg cgccggcacc accagcaccg agagaatgag cccgcgcgcg gacggaattt
     1021 cctcgaaccg gcacgatagc ccggtcagat ccggctgcgt gccggggggc gccgccggca
     1081 ccgcaaactc gccgcgcttc atcgcggttt cggcccagcc gaggccgccg ccggagaaca
     1141 tcgcatagga cagattaggc gacggcccga accgcgccac gcgaacgtca aaaccctgcg
     1201 cgcggacggc tgcgaccggc accagcgcca cccgcatcgc gagattgagg tcttccttga
     1261 cccaattcgc ggtcgccgcc agcgcctcgc gcgcccgcgc gagatcctca tgcgacaccg
     1321 caaagcttgc accgtcgccg ccgaacacga atggaaattc gcgtcccgca agcgcattcg
     1381 tgaccgcggc gatcaccgaa gcgccggcca tgttgaccgc cttgtaacgt gcttccgcga
     1441 tcgccttggt cgattccacg atatcggcga cgccgatgct ccagtcatcc ggcaacggcg
     1501 aatacagcgc cggatccatc aggcgggaaa agccgcgaaa caccggaatc ttgctgtaga
     1561 aggtgtcgtt gggcgtggtc atctcaaatt acccagttac gaaaccgtcc agagccccgt
     1621 ttcgatagaa tcgaaacggg gctctagatt tttaatttga cgcgttttct tgacgcgaac
     1681 cggtccccac ttcgcttgaa aacgctttag cagatacggc cggccccgta atcaggttca
     1741 agactgctgc cgctccaatg gacttcgacg gctggccgtt gcattttggc accgatccga
     1801 aagccgatgg gagagcggcg acttctccgc accagatgga cgctgatatg ccggtggatt
     1861 tgagccgagt gaacaaaaac accgccatgg gggccacggt gcgggccaat gggacgggct
     1921 ttcgtacctg ggcgccgaat gcaaagcagg tttacgtggt gaccggcagc gcacttgcag
     1981 cgcaccacga accgtcctgg cgcccggccc cggaacacgg tttgacgccg ctcggtgacg
     2041 gaagctgggg cggctttctg gccggagtgg gcgacggcga gccttacatg tttttcgttg
     2101 aggggatcgg cagcgctgga tggaagcggg atgcttatgc gcgcgagctc agcctgtcgc
     2161 ccgccttccc cgacagcacc tgcatcgttc gcgatcctca aaactattcc tggcgcgacg
     2221 aaacctggca gccaccaaaa ttcaacgatc tcatcatcta tcagcttcat gtcggcacct
     2281 ggtgggcgca ggacgagacc ggccgcgatg tccgcaagct gcgcggcggc acgtttctgg
     2341 acgtcgtcga aaaacttgaa catctgcaac atctcggcgt gaacgccatt caactgctgc
     2401 ccattcagga attcgagacg gcattcagca tgggctacaa tggcgtcgac tatttttcgc
     2461 cggaaggcgc atatgccgtt gcccccgagc aactcggctg gcggcttgcg cgtatcaacc
     2521 gaatgctcga acgcttcggc aagcagcctc tgacggctcc cgaacttgcc cactgcacca
     2581 atcagttgaa atgtcttgtc gacctctgcc atctgcacgg catcgccgtg attttcgatc
     2641 tggtctacaa ccacgccggc ggcggtttcg acgatcgcag catctggttt tacgaccggc
     2701 agccgagcga cgaccagaac aacagtcttt tcttcacgga tcatggctgg gccggcggtc
     2761 aagtcttcgc ctattggaat gattgggtag cgcagttcct gatcgacaac gcctgcttct
     2821 ttttgaccga gtttcggatc gatggaattc gctacgatga ggtgcgggtg atcgagaaca
     2881 atggcggccg cccgttctgc cagcgcctga cgaacgcggt gcgggcgacc aatcccgcgg
     2941 cgatccagat tgccgaatac tggaacgagg atcgcgccag cgcgctgcgc ccgcctcccc
     3001 gcggactagg tttcgacgcg gagcttggcg atagtctgcg cgatgcgctg cgcgacctgc
     3061 tcagacaggc ctccgccggt gcctcggcga cgctggatct ttcagaggtg gccaacgccc
     3121 ttgtcgtgcc ggctgcgatt cccgaggcct ggcgtctggt gcaatgcctc gaaaatcagg
     3181 acctgacgtt tgcgtcgcat tcggatgccg cgcgggtcgc gatgctggcg gactcgctgg
     3241 accgaacctc ctggtacgcc cgcagccgaa gccggacggt caccggcctg ttgctggccg
     3301 cgcccggaat tcccgccttg ttcatgggcc aggaattgct tgaggacaag aactggagcg
     3361 acaaccggca gaccgatggc ctcaccggat gggatgcgct cgatggcgct gatacctcaa
     3421 ggcgcgactt tctaagattc gtcaccgaca tgatcgagct tcgacgttcg cagccggcgt
     3481 tgcgcggtag cggcgcgcgg gtgccgcgcg ccgaagattt cgagcgggtg atcgttttgc
     3541 acagatggat cgagggagaa ggccgggatg tggtcgtgat agccaatctg gccgagcaac
     3601 cgaaacaggc ttatgcgatc gggctgccgt ttgccgggga ctggtccgaa ctgctcaaca
     3661 gcgatgtcta tgacaacttt ccaaatcccg cgccggtcgg aaatggcggc cacgtgcgtg
     3721 cctggggcga gccgttggat ggcttttcgg cgagggccgc cgtcacgctt cccgccaacg
     3781 gcgtgatcgt gctcgcgcct tcaagggcgc gttaaccacc attgagcgca ccgcatcacg
     3841 ccacaagcgc tacattccgg aacgccttca ccagcgctag ttccagcttt ccgtccatgc
     3901 cgcgcaggat cttgtaggcg gtttggcggg gcatggccgg tcggtacggc ctcgactcaa
     3961 tgagggctgc gaaaatatcc gagatcgtca gcattcttac cagatcggaa atctccggcg
     4021 ccgtcagcgc atcgggataa ccagagccgt ccagatattc gtggtggtgc cttacgccgt
     4081 caagaacctc gggggtgatg tctgggatat ccttcaataa ttcatatccg ataaccgggt
     4141 gccgtctcat gatctcttct tcgcctggat caaggcgatc gggcttgtcg agaaccaaca
     4201 ggggaatgcg ggctttgccg atgtcatgaa gggttgccgc gattcccaat cgctttacat
     4261 ccaaactgga gaacccgatc tccaacgcaa acccgactgc cactcccgtc accagcagac
     4321 aatgctggaa ggttccttcg tgatagcggc gtacgtcgtc gagccaggcg gcaagccctt
     4381 tttccgaaat gctgttgatg atttccaaag tggcgcgctc ggcatccgac aggtttattt
     4441 gtttgccggc ccgtaccgcc gaaaacatcg acgcgaaggc cgccgtgctc tctgtgatct
     4501 cgggtgaggt aatggcaaag tcgctttgtg ctgccttctc ggcgatttcg atctgcgcca
     4561 gtttgaagat gatttctttt ggacgcgaaa cgaccgcggt cgctccgagc gcataggcct
     4621 gtgcaaccat ggagcgaacg cggttatgaa cgacaaacag cttttcggaa atgcaactca
     4681 gctcctgcaa gacaagcctg atctgttcga cgcgatcgat cttgcgcagg tcggcgtcaa
     4741 ccatcagcac gccgttggaa tggacttgcg cgtcgcccat tcccaacagt tggggggcaa
     4801 cgtgatatcg cggttccaac atcgcgcgga tggccggtat tttcgcagcg tcatcggtga
     4861 cgaaatgaac aagcattgca gggccagacc ataaaaaact gaagcgtagc ctccacttca
     4921 aagcagcaat actaacgatg gcccaccatc gtgcgggaac ctgcctaact tcccaatcga
     4981 gggttaacga cttattaaga ctgtcggacc agcggtatcc tgtgccgggt ttatcgatct
     5041 cggttgcgcc aattcgaact tgccctctgg ctatatcgcc tggccgctcc ccttcgtcat
     5101 cccgcgcagc accagctggt tgagccggtt ggcaaaggcg gcgggatctt ccggcaactc
     5161 accgtcgaga atttgcgcct gttccagcag cagaaacgac aaatccgtgg cggcgtcctg
     5221 ggtcccggaa atggccgcga ccagcggatg gcgcatgttg atttcgagga tcggtttggt
     5281 gccggcgcct ctgttctgcc gtgccagcaa tcgctcgagc tcgcggtccg ggccgtggct
     5341 gccggcgacc aggcatgacg cactggacgt cagccgttgc gaggcgcgca cgtcggcgac
     5401 gcggtcgccg agggcggcct tgatcacggc gatggtcgcg gcctcgtcgg tgtccgattt
     5461 ggattcgtcc ttgctgtcgt cgatgcgcgg gatcaggcca aagtcgatgt cgccctggct
     5521 cagcgatttg agcggcttgc cgtcaaaatc gagcggcgcc gaggtccaga acgcgtcgac
     5581 cggatcggtc agcagcaaaa cctcgatgcc gcgggcggcc gcctgctcca gcttgggatt
     5641 ggatttcagc cgctcgatgc tgtcgccggc cagaaagtag acctcggtct gattgggttt
     5701 caggtccgcg acatattgct tcagcgagcg cttctcgccc gcg
//
LOCUS       DASWGM010000983         6446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1083901,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000983 DASWGM010000000
VERSION     DASWGM010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083901"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1927
                     /locus_tag="VGA15_26500"
     CDS             <1..1927
                     /locus_tag="VGA15_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX9591281.1"
                     /translation="FTCYKSLADLPEAPEMVIVAVAGDNAIGAVEDCAAHGVKVAVVM
                     ASGFGEVDAVAGKAKERRMVDAAQKTGMRIVGPNSQGLANFGTGAIASFSTMFMDMER
                     SEGHVAMLSQSGALSTVPVGFLRQRGIGVRHTHATGNDADISVGELAVAVAEDPEVKL
                     LLLYLESIPEKKYLEELAAVALDRDLPIIALKSGRSEAGRQAAQSHTGALANEDRVVD
                     AFFEHHGIWRAPDMRGLVEATELYLKGWKPKGRRLVAISNSGAVCVLTADAATAVGMP
                     MAKLAPETDKKLKGILPSFATTTNPIDLTAALLSNGRLFGDILPVIAEDPAADAFLIG
                     IPVAGPGYDVEAFARDSAAFGRQTGKPLVIAATQRSVADQFASEGLTVFPTETEAVTA
                     LHQFLAHRELMARTLARKATRVAASPPSRSAETTMLNEADSLALLAARGIPVVPHRLC
                     RSRAEAAAAFESIGGPVVVKGCSADIAHKSELGLVKLGVRTREEAGEVWAQMEGIIRK
                     HGARFDGVIIAAMAGGRREIMIGAHRDPVFGPLVAVGDGGKYVEIFRDTALLLPPFSK
                     DDAKEALGKLRIAPLFKGVRGEPPMDVDSLCDAVVRIGELMLDPAAGVMSLDLNPVLL
                     DSAGKGCVVVDAVVFRE"
     gene            2597..3121
                     /locus_tag="VGA15_26505"
     CDS             2597..3121
                     /locus_tag="VGA15_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9591282.1"
                     /translation="MARIDYSDPANASDRTREILGKNRNANIFRMMAHSPSYFEQYCR
                     LGGAIRHKGELDPVVRELAITRTGILCEAPYEIVAHKRIGKNVGVTDEQNAALEDWKS
                     ATCFSDVQRAALAFTDEIVAMRRPTDATFNAIAAKLTPAALVELQLSVGFYIMTSKFL
                     ETFDIDMQPVAEVV"
     gene            complement(3137..4996)
                     /gene="glsA"
                     /locus_tag="VGA15_26510"
     CDS             complement(3137..4996)
                     /gene="glsA"
                     /locus_tag="VGA15_26510"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEX9591283.1"
                     /translation="MKPAPSASAARATAWAAAPPPLLRFLAACHADFSTETGGTVADY
                     IPELGKADPEHFGISLATLDGHVYEVGDTRIPFTIQSMSKPFVFALALDTLGAARVES
                     AIGVEPSGDPFNSIRLNADNHPFNPMVNAGAIACSGLIHEAKGDGAFEYIRQALGRFA
                     GRELDIDEAVFASESATGDRNRAIGYLLRNSTVIKDDVSAVLDVYFRQCSVLVTARDI
                     AIMAATLSNRGINPVTGEQVMTPYAISRTLSVMTSSGMYDYAGEWIYRVGIPAKSGVG
                     GGILAALPARLGLGSYSPRLDRHGNSVRGIKVCEALSRHYDLHMLNRSDDARNSIIAD
                     YNIGKSPSRRSRRPHEQKILAAHHQDVRIIELVGTLTFSSIDYVSRQLAAKLRPQFVI
                     FDLRRVAAVTRAGARLLAEEFRELAHLHVIVILSGIKRSSADWKMLKEWTGGISNLRD
                     FYLLDAAIEWAEDQVVYRHGGAIDFHEVTELSEQPLLAGLTPEELAGLAALGTIRSYR
                     PGEKIVAAGEPATSLFFLKSGVVHVTLPDGIRLATLTAGMAFGEMALLETHRSADVLA
                     DVSATALELPLREFERFRDQHPRIGERIMRNLAQLLAERLIVANTKVDLLTAK"
     gene            complement(5054..5275)
                     /locus_tag="VGA15_26515"
     CDS             complement(5054..5275)
                     /locus_tag="VGA15_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX9591284.1"
                     /translation="MPDIKIVTEVAGRVCALPVEVGGCVGDGDDIAFVEAMKMEIPVA
                     APAAGKLKTILVSLDDVVAEGQVLAILET"
     gene            5466..5645
                     /locus_tag="VGA15_26520"
                     /pseudo
     CDS             5466..5645
                     /locus_tag="VGA15_26520"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1184 a         1980 c         2117 g         1165 t
ORIGIN      
        1 ttttacctgc tacaagagcc tcgccgatct gccggaagcc cctgaaatgg tgatcgtcgc
       61 ggtcgccggc gacaacgcca tcggtgcggt ggaggattgc gccgcgcatg gcgtcaaggt
      121 cgcggtggtg atggcgtccg gtttcggtga ggtcgatgcc gtcgcgggca aggccaaaga
      181 gcgccgcatg gtcgacgccg cgcagaaaac cggcatgcgc atcgtcggcc cgaattcgca
      241 aggcctcgcc aatttcggca ccggcgccat cgcgagcttc tccaccatgt tcatggatat
      301 ggagcgatca gaggggcacg tcgcgatgct cagccagagc ggcgcgctat ccaccgtgcc
      361 ggtcggattc ttgcgccagc gcggcatcgg cgtccgccac acccatgcca ccggcaatga
      421 tgcggacatt tccgtcggcg aacttgcggt cgcggtcgcc gaagaccccg aagtaaagct
      481 gctgctgctt tacctcgaga gtattcccga gaagaaatat ctcgaagaac tcgccgccgt
      541 cgcgctcgac cgcgatctgc cgattatcgc attaaagtcg ggccgctccg aagccggccg
      601 ccaggcggcg caatcgcaca ccggcgcgct tgccaacgag gatcgcgtgg tcgacgcctt
      661 cttcgagcat cacggcatct ggcgggcgcc ggacatgcgc gggctggtcg aggcgaccga
      721 gctttatttg aagggctgga aaccgaaagg aaggcggctg gtcgcgatca gcaattccgg
      781 cgccgtctgc gtcttgacgg cggacgcggc aactgccgtc ggcatgccga tggcgaagct
      841 cgcgccggag accgacaaga agctgaaagg catcttgccg agttttgcga cgacgaccaa
      901 cccgatcgac ctgaccgcgg cgctgttgtc caacggccgg ctgttcggcg acatcctccc
      961 cgtcatcgcg gaagatcccg cggccgatgc gttcctgatc ggcattcccg tcgccggtcc
     1021 cggctatgac gtcgaggcat ttgcgcgcga ctccgcggcc ttcggcaggc agaccggaaa
     1081 gccgctggtg atcgccgcga cgcagcgcag cgtcgccgat caatttgctt ccgaagggct
     1141 gacggtgttt ccgaccgaga ccgaggcggt gacggcgctg catcagttcc tggcgcatcg
     1201 cgaactgatg gcgcgcacgc tcgcgcgcaa agcgacgcgt gtcgcggcca gcccgccatc
     1261 tagatccgcc gagacgacga tgctgaacga ggccgacagc cttgcgctgt tggctgcgcg
     1321 cggcattccg gtggtgccgc accggctgtg ccggtcgcgc gccgaagcgg cggcggcgtt
     1381 cgagtcgatc ggcggtccgg tggtggtcaa gggatgctcg gccgatatcg cgcacaagtc
     1441 cgaacttgga ctggtgaagc tcggcgtcag gacccgtgag gaggccggcg aagtctgggc
     1501 gcagatggag ggaatcattc gcaagcacgg cgcgcggttc gacggcgtca tcattgcggc
     1561 gatggccgga ggccggcgtg aaatcatgat cggcgcgcac cgcgatcccg tgttcggtcc
     1621 gttggtggcg gtcggcgacg gcggcaaata tgtcgagatc tttcgcgata ccgcgctgct
     1681 gctgccgcca ttttcgaaag acgacgcgaa ggaagcgctg gggaagttgc gaatcgcgcc
     1741 gctattcaaa ggcgtccgcg gcgagccgcc gatggacgtt gattcgctct gcgatgcggt
     1801 ggtccgaatc ggtgagctga tgctcgatcc cgcggctggg gtgatgagcc tcgatctcaa
     1861 tccggtgctg ctcgacagcg ccggcaaggg ctgcgtcgtg gtggatgccg tggtatttcg
     1921 ggagtagtcg gagtttcgtc accggcggac cgtcgtccgg cgagttgtgg ggtgaggtgt
     1981 gactcaaaac taactacacc ccaccccgtc tcgcatcacg cctggcgtga cgctcatccc
     2041 ccctggggag gaaaccgagg agcatcttgt tgtttctgca agcgcctcaa aatattcaaa
     2101 ccttcaccgc gtgtagaaga tgaagctggc gatgacgagc attgcagtca ccgccagcat
     2161 ttgcgcaaac gcttccgagg ccgacctttt ggtggcttcc ttcatgccat tctccctaga
     2221 cctgggcacg agtcatcgtt gcccggcgaa ggctggatgg ctcaaacatt tttactcgga
     2281 acacccctcg acgatattcg ctttgatgac tccccagtca gcgaacatcg acggtgttaa
     2341 aatcgattag caatacggcg ggaatttggc tcacgcgtag cggacttgtg cacgtgccgt
     2401 gacgtttttg gagcgatttt gactcccaaa gttggcgtgc atgctcaaga ccaccgtgcc
     2461 gcgtcgcgcc aaatgctcca tttgccggcg cacaagccgt tgatccggcg ttgggcgcaa
     2521 ggcatgatcg ggcgctcggc gcggtgcttt tggcatgcaa gcccaaccaa tcaaacgaaa
     2581 aaccccaagg tgaaccatgg cccgcatcga ctattccgac cccgccaatg ccagcgaccg
     2641 cacccgcgaa atcttgggca agaaccgcaa cgccaacatc ttccggatga tggcgcattc
     2701 gccgagctat tttgagcaat attgccggct cggcggcgcg attcgccaca agggcgaact
     2761 cgatccggtg gtgcgcgaac tcgccatcac ccgcacggga attctatgcg aggcgcctta
     2821 cgagatcgtt gcgcacaagc gtatcggcaa gaatgtcggg gttaccgacg agcagaatgc
     2881 cgcgctggag gactggaaat cggctacctg ctttagcgat gtgcagcgcg cggcgctggc
     2941 cttcactgac gaaatcgtcg ccatgcgccg tccaaccgac gcgacgttca atgccatcgc
     3001 tgcgaagctg acgccggcgg cgctggtcga actgcaactc tcggtcggtt tctacatcat
     3061 gacgtcgaag tttttggaaa ccttcgacat cgatatgcag ccggtggccg aggtggtttg
     3121 aaagacgatc gcgtttctac ttcgcggtca gcaagtccac cttggtattg gccacgatca
     3181 ggcgctcggc gagcagctgc gcgagattgc gcatgatccg ttcgccgatc cgcggatgct
     3241 ggtcgcgaaa gcgctcaaac tcgcgcagcg gcagttcgag ggcggtagcg gatacatccg
     3301 ccagaacatc cgccgagcga tgtgtttcca acagcgccat ttctccgaac gccattcccg
     3361 cggtcagtgt cgcaagccgg atgccatcgg gcagcgtgac gtggacgacc ccgctcttaa
     3421 ggaaaaacag cgaggttgcc ggctcgccgg cagcgacgat cttctccccc ggccggtagc
     3481 tccggatcgt gcccagcgcg gctaggcctg caagttcttc cggcgtcagt ccggcgagca
     3541 gcggctgttc ggataattcg gtgacctcat ggaaatcgat cgcgccgcca tggcgataga
     3601 cgacctggtc ttccgcccat tcgatcgcgg cgtcgagcag gtagaagtcg cgcaagttcg
     3661 agatgccgcc ggtccattcc ttgagcattt tccaatccgc ggaggaacgt ttgatgcccg
     3721 acagaatcac gatcacatgg aggtgcgcca attcgcggaa ttcctcggcg aggagccggg
     3781 cgccggcgcg cgtcaccgcc gcaacccggc gcaggtcgaa aatgacgaat tgcgggcgca
     3841 gcttggccgc gagctgacgc gaaacgtaat cgatgctgga aaatgtcagc gtgccgacca
     3901 gctcgatgat gcggacgtcc tggtgatggg cggcgagaat cttctgctca tgcggccggc
     3961 ggctgcgccg cgacgggctt tttccgatgt tgtagtcggc gatgatgctg ttgcgcgcgt
     4021 cgtcgctgcg gttgagcatg tgcagatcgt agtgtcgcga cagcgcctcg cagaccttga
     4081 tgccgcgcac gctgttgccg tggcggtcga gccggggcga gtagctgcct agcccgagcc
     4141 gcgccggcag cgccgccagg atgccgccgc cgacgccgct cttggccggg atgccgacgc
     4201 gatagatcca ttcgccggcg tagtcgtaca tgccggaaga agtcatcacc gaaagcgtcc
     4261 gcgagatggc gtagggggtc atgacctgct cgccggtcac gggattgatg ccgcggttgg
     4321 agagcgtcgc agccatgatg gcgatatcgc gcgcggtcac cagaaccgag cattgccgga
     4381 aatagacgtc cagcaccgcg ctgacgtcat ccttgatgac ggtggagttg cgcaggagat
     4441 agccgatcgc gcggttgcgg tcgccggtcg cactttcgga cgcaaacacg gcttcgtcga
     4501 tgtcgagttc acgcccggca aaccggccga gcgcctgacg gatatattcg aatgcgccgt
     4561 cgcccttggc ctcatgaatc aggccggagc aggcgatggc gccggcgttg accatcggat
     4621 tgaagggatg gttgtcggca ttgaggcgga tcgaattgaa ggggtcgccg gacggttcga
     4681 cgccgatcgc gctttccacc cgcgccgcgc ccagcgtatc cagcgccagc gcgaacacga
     4741 acggcttcga catcgactgg atcgtgaagg ggatcctggt atcgccgacc tcgtagacat
     4801 gcccgtcgag ggtggcgagg ctgatgccga aatgctcggg gtcggccttg ccgagctcgg
     4861 ggatatagtc ggcgacggtg ccgccggtct ccgtggaaaa atcggcatga caggcggcga
     4921 ggaaccgcag caaaggcggt ggcgcggcgg cccaggccgt ggcccgggcg gcggatgcgg
     4981 agggtgctgg cttcatcgcc tgccgttgtg ccgcgcaatc atggcgcgcg caacaggccg
     5041 gcgtcgtcgt cgctcaggtc tcgaggatgg cgaggacctg tccttcggca accacgtcgt
     5101 cgagactgac aagaatggtt ttaagtttcc cggctgccgg ggcggcgacc gggatttcca
     5161 tcttcatggc ttcgacgaat gcgatgtcgt cgccatcgcc gacgcatccg ccgacctcaa
     5221 ccggaagcgc gcagacgcgc cccgccacct cggtcacgat cttgatatct ggcatggcgt
     5281 tctccggggg cagccgccgc gcgccatcgc gattggaata cgcgcacatg aaggcgggcg
     5341 ccctcggctt gtggccgcag attgcctgag gtagcttgat ctgcaagtgg aattttattc
     5401 cgcataacgg aattagggcg gggcaccaca tgggacgacg ctcggaacgg ctcagcaagc
     5461 agggaatgct cgccagcgat cttgcaggcg acggcgacgt tatccaggtg gtgtcacgcg
     5521 cgttcgacgt gttgcgctgt ttcgagggcc acgaggcacg gctcggcaat ctggaaattt
     5581 ccaatcgctg cgggctgccg cgctcgacgg tgtcgcgcct gacccacacg ctgacccgga
     5641 tgggccagct cgtctatctg ccgcgcgacc agaagtaccg catcggcccg agtgcggtgg
     5701 cgatgagcac ctcgatgatg cgggggctgc aactgcgcaa cctgatccgg ctgcggctgc
     5761 aggacgtcgc cgagcaactg ccgggaaccg tcgggttcgt cattccggat cgctttcatc
     5821 tggtctatct cgagttcgcc cgggccgcca acgcgctcgg tctgcatgaa agcaccggca
     5881 gtcgcatcgc catggcgagc acggccgcgg gccacgccta caccgccgcg ctcgacgtcg
     5941 atgtcggcga cgccctgctg gcggaaatgg agcgtgaaat tccggcgggc gccaaactgc
     6001 tgcggccccg catcgaggcc aatcgccgct tcttgcgcga acacggctat gtcgttgcct
     6061 gcggcctgtg gagtccgcat atcaacggtc tcgcggtgcc gctatggtcg ccgcaatatc
     6121 agaccttcgt ggtcgtcacg atcggccttc tgtcggccat gtatgacgaa aagcggctgc
     6181 acaaggaggt ggcccctcgg atgctggaac tgggcgcggc ggtcggcacc acgctcgagg
     6241 gcaccgaagg agatatcttc agcaaccggc tggagagaaa acagcttccg gtaccggccc
     6301 caaataataa aatcatcaag acggaggata cgaatgaact ggaagccgga actcgacgac
     6361 ctcgcccggc gcgaagcgtt cgcgcgcgag atgggaggcg ttgacaaggt caagcggcag
     6421 cgcgaccagg gccggctcac cgttcg
//
LOCUS       DASWGM010000984         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1222745,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000984 DASWGM010000000
VERSION     DASWGM010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222745"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /gene="maeA"
                     /locus_tag="VGA15_26525"
     CDS             <1..1116
                     /gene="maeA"
                     /locus_tag="VGA15_26525"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HEX9591285.1"
                     /translation="AVPPDCLLPIHLDIGTTNAALRADPLYAGLRDEPPSPDEVDALV
                     DEFVEAANRVFPGVCVHFEDWKGSDAIRLLARYKDRYLVYNDDIQGTASVTIAGLTTA
                     LQIKDEKLADQTVLFAGAGSAGIGIANMIVEAMVDEGRSREDARKRVTLFDVNGLIEA
                     SRKDLNPSQAVYARDLKPTRDLAEAVSDLKPTILIGVSTVGGLFTEAVIRNMAKTVDR
                     PIIFPLSNPTEKAECTPEQAYRWTDGKALVAAGVQFPDVTIDGKTLHPGQANNFYIFP
                     AIGLAVYATRPKRIDDRMFIEAARGSADQVSQAERDKGMLYPRQDDILETEITTATRV
                     AEYIFEQGQATVDRPDDIRAWIKAMTYKPVYCTGHKT"
     gene            1172..2632
                     /gene="fumC"
                     /locus_tag="VGA15_26530"
     CDS             1172..2632
                     /gene="fumC"
                     /locus_tag="VGA15_26530"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007630910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX9591286.1"
                     /translation="MADPNKDHPIIKDVPIGLSAGGSRREFDSMGDVDVPADRYWGAQ
                     TQRSLQHFSIGRDRMPKEVYHAYGIVKKACALVNQVAGRLLTWKAEAIVRAADEAIAG
                     DLDEHFPLFVWQTGSGTQSNMNVNEVLSNRAIQLLGGELGSQQPVGPNDDVNMSQSSN
                     DSFPTAMHIAAVTLLDEHLIPELDRLATVIETKARDWMDVVKIGRTHLEDAVPLSVGQ
                     EWSGWAAQIRACITDIERSREGLYQLALGGTAVGTGLNAPEGFSKQVAAKIAELTNKP
                     FVTAPNKFMAQGSLDAMVRASAALRGAAVALMKIANDMRWLASGPRCGLGELELPQNE
                     PGSSIMPGKVNPTQCEAMVMIAIQVIGDDTAVAFAGSQGNFELNAMRPVIINNFLHSA
                     TILADGCEKFRRFSVEGTELDRERIDGYVGNSLMLVTALSPVIGYQNAAHIAEKAAAD
                     GTTLREAAIASGKVRADEYDRIIVPKTMIGSGVGGA"
     gene            complement(2794..3318)
                     /locus_tag="VGA15_26535"
     CDS             complement(2794..3318)
                     /locus_tag="VGA15_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX9591287.1"
                     /translation="MQVRVRDAKAGPAPLHTPSDLKPNAVRDRSGALNILLADMFVLY
                     LKTKNFHWHMSGPHFRDYHLLLDEQGDQIFATTDAIAERVRKIGGTTLRSIGHVGRLQ
                     RVADNDSDFVTPMDMLAELRDDNKQLVANLRETHGLCDEHGDVATASLLEIWIDEAER
                     RTWFLFEATRRAEP"
BASE COUNT          684 a         1069 c         1072 g          601 t
ORIGIN      
        1 gcggttccgc ctgactgcct gctgccgatc catctcgaca tcggtaccac gaacgcagcc
       61 ctccgggccg acccgctcta cgcaggtctc cgcgacgagc ccccttcgcc cgatgaggtc
      121 gacgctctgg tcgacgagtt cgtcgaggca gcaaatagag tgttcccggg tgtctgcgtc
      181 cactttgagg attggaaggg atcggatgcg atccgcctgc tggcgcgcta caaggacagg
      241 tacctcgtct acaacgacga cattcagggg acagcgagcg tcaccatcgc tggcctcacg
      301 acggcgctcc agatcaagga cgagaagctc gctgatcaga ccgtgctgtt tgccggcgcc
      361 ggatcagccg gcatcggcat tgcaaacatg attgtggagg cgatggtcga cgaagggcgg
      421 tcgcgggagg acgcgcgcaa gcgcgtgacg ttgttcgacg tcaacggctt gatcgaagcc
      481 tcacgaaagg atctcaaccc gtcgcaggcg gtctatgcgc gcgacctcaa gcccacgcgc
      541 gacctggcag aggcggtatc agacctgaag ccaaccatcc tcattggcgt gagcacggtg
      601 ggtggtctct tcaccgaggc ggtcataagg aacatggcga agacggtcga tcgcccaatc
      661 atcttcccgc tgtcgaatcc gaccgagaag gcggagtgca cgcccgaaca ggcttataga
      721 tggaccgacg gcaaggcgct ggttgcagcc ggggtgcaat ttcccgacgt gacaatagac
      781 gggaagacgt tgcaccccgg ccaggcgaac aacttctaca tttttccagc tatcggcctg
      841 gcggtttacg ccacaaggcc gaagcggatc gacgaccgca tgttcataga ggccgcccgc
      901 ggcagcgccg accaggtgag ccaggcagag cgcgacaagg ggatgctgta cccgcgccag
      961 gacgacatcc tcgaaaccga gattacgacc gcgacccggg tcgccgagta tatcttcgaa
     1021 caaggtcagg cgacggtaga ccggccggac gatatccgcg cctggatcaa ggcgatgacc
     1081 tacaagccgg tctactgcac cggtcacaaa acttgaggct ccaccgccgc gggtcgtgac
     1141 ccgccgcgac caagttcacg agagatcggc tatggccgac ccgaataaag accacccgat
     1201 tatcaaggat gtcccgatcg gcctatcggc aggcggcagc cgtcgcgagt tcgacagcat
     1261 gggcgacgtc gacgtgccgg ccgaccgcta ctggggagcg cagacccaac gctcgctgca
     1321 gcacttctcc attgggcgcg accgcatgcc caaggaggtc taccacgcat acggcatcgt
     1381 gaagaaggcc tgcgccctgg tgaaccaggt ggctggccgc ttgctcactt ggaaagcgga
     1441 ggcgatcgtg cgggccgccg acgaggcaat cgccggcgat ctcgacgaac atttcccgtt
     1501 gtttgtctgg cagaccggtt cgggcacgca gtccaacatg aacgtcaacg aggtgctgtc
     1561 gaaccgcgcc atccagctat tgggcggcga gctgggctca cagcagccgg tcggccccaa
     1621 cgatgacgtc aacatgagcc agtcgtccaa cgacagcttc ccgaccgcga tgcacatcgc
     1681 cgcggtgacg ctgctcgacg agcatcttat ccccgaactc gaccgactgg ccacggtgat
     1741 cgagaccaag gcccgcgatt ggatggacgt ggtcaagatc gggcgcaccc atcttgagga
     1801 cgcggtgccg ctgtcagtcg gccaagaatg gtcgggctgg gccgcgcaga tccgtgcatg
     1861 catcaccgac atcgagcggt cgcgcgaagg tctctaccaa ctcgcgctag ggggcaccgc
     1921 cgtcggcacc ggtctgaacg cgcctgaagg cttctcgaag caagtcgccg ccaagatcgc
     1981 tgagctgacc aacaagccct tcgtcacggc gcccaacaag ttcatggccc agggttcgct
     2041 ggacgctatg gtgcgggcgt cggcggcatt gcgcggcgcc gcggttgcat tgatgaagat
     2101 cgcgaacgac atgcgctggc tggcgtccgg tccgcgctgc ggcctgggcg agctggagct
     2161 gccccagaac gagccgggct cgtcgatcat gccgggcaaa gtgaacccga cgcagtgcga
     2221 ggcaatggtt atgatcgcga tccaggtgat tggcgatgat actgcggtgg cgttcgcggg
     2281 gagccagggc aacttcgagc tgaacgcgat gcgacccgtc atcatcaaca actttttgca
     2341 ttcggcaacc atcctcgccg acggctgcga gaagttccgc cgtttctcgg tcgagggcac
     2401 cgagctcgat cgtgagcgga tcgacggcta cgtcggcaac tcgctcatgc tcgtgaccgc
     2461 gctgtctccg gtgatcggct atcagaacgc cgcgcacatc gccgagaagg cggcggccga
     2521 cggcaccacg ctgcgggaag cggccatcgc ctcgggcaag gttcgggcgg acgaatatga
     2581 ccggataata gtccccaaga cgatgatcgg cagcggcgta ggcggcgcgt gaagcatcga
     2641 aaaaggtgct gctgcaggtc cgcgatggtt cgcgatcctc tgtcagctgg gcacgtcggc
     2701 ggcagtcgcc gccttcgagg tcggcttgcg ccctcccggg cgtcactgat ctaatgatca
     2761 ttcgtacctg gcgaatgttc gagtgctttc gccctagggt tcagcccgcc gcgtggcctc
     2821 gaacaggaac catgtgcgcc gctcagcctc atcgatccag atttccaaca agcttgcggt
     2881 ggcaacatcg ccgtgctcgt cgcaaaggcc atgcgtctcg cgcaggttcg ccaccaattg
     2941 tttgttgtca tcgcgcaatt cggcgagcat atccatcggc gtaacaaaat ccgaatcgtt
     3001 gtctgcgacg cgttgcaagc ggccaacgtg gccgatcgag cgcaaggtcg tgccgccgat
     3061 cttgcggaca cgctcagcga ttgcatcggt agtggcgaag atctggtcgc cttgctcatc
     3121 cagcagaaga tgatagtcgc gaaaatgcgg acccgacata tgccagtgaa aattcttcgt
     3181 cttcagatac agaacgaaca tgtcggcgag caggatattc agcgcgcccg agcgatcccg
     3241 tacggcattc ggcttcaggt cgcttggcgt gtggagcgga gcgggccctg ccttggcatc
     3301 tcgaactcga acttgcattt tttgtctcct tccgtgtcat tgagtgcccg aagcgacgaa
     3361 cgcatcctgc tcatccctcc agcacatcct cgatccggat cggcaatttg cggacgcgga
     3421 tacccg
//
LOCUS       DASWGM010000985         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1222875,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000985 DASWGM010000000
VERSION     DASWGM010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222875"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VGA15_26540"
     CDS             complement(<1..845)
                     /locus_tag="VGA15_26540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX9591288.1"
                     /translation="MRILVATDAWHPQVNGVVRTLTNMAEAARAFGVEISFLTPQSFR
                     TFAMPSYPDLRLALPYQAKIARLIAEARPDSIHIATEGPIGLLVRRYCRKNQLPFTTS
                     FHTRFPEYISARLPIPEAWIWRALRAFHGPSQAVMAATPALAGELRARGFRNVVLWPR
                     GVDAGQFHPRAADLGLPRPVFLCVGRVAVEKNLEAFLDLDLPGTKVIVGDGPARAALT
                     RKYPQAVFLGARQGKELAEAYAAADAFVFPSKTDTFGLVLLEALASGLPVAAFPVTGP
                     RDVIG"
     gene            complement(845..>1536)
                     /locus_tag="VGA15_26545"
     CDS             complement(845..>1536)
                     /locus_tag="VGA15_26545"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX9591289.1"
                     /translation="HDADTIYLVGDIVDGWALQSSWYWPQSHNDFVQKMLRKARKGAK
                     IVYVPGNHDEFLRNYYGTHFGGIDVVENTIHVGVDGQRYLVIHGDIFDLVVQNARWLA
                     HLGDKAYDFAIQMNRIVNAFRRWFGVPYWSLSQWAKLKVKNAVNYIGAFEQTLAAEAR
                     RHGADGVICGHIHYATIRDEHGIRYMNCGDWVESCTALAEHEDGRFEIITWTDPLRRT
                     APVTSVAARAA"
BASE COUNT          297 a          496 c          480 g          263 t
ORIGIN      
        1 ttgccgatca cgtcgcgagg accggtcacg ggaaacgcgg caaccggcag gccgctcgcg
       61 agggcctcga gtagaacgag gccaaaagtg tcggtcttgc tcgggaacac gaaggcatcc
      121 gccgccgcat aggcctccgc cagctccttg ccctgacgcg cgccgagaaa caccgcttgc
      181 ggatattttc gggtcagcgc cgcgcgcgcc ggcccgtcgc cgacaatcac cttggtaccg
      241 ggcaaatcga ggtccagaaa cgcctcgagg ttcttttcca ccgcgacacg gccgacgcac
      301 aggaagaccg ggcgcggcag gccgaggtcg gcggcgcgcg gatgaaactg gccggcgtca
      361 acgccgcgcg gccacaacac gacgtttcga aacccacgcg cacgcagttc gccggccagc
      421 gccggcgtcg ccgccatgac cgcctggctg ggcccatgga aggcgcgcag cgccctccat
      481 atccacgctt cggggatcgg caggcgcgcc gatatatatt ccggaaagcg ggtgtgaaag
      541 ctcgtggtga acggcaattg attcttgcgg caatagcgcc gcaccaacag gccgatcggc
      601 ccctcggttg cgatgtgaat gctgtcgggc cgcgcctcgg cgatcagccg cgctatcttg
      661 gcctgatacg gcaacgccag ccgcagatcc ggatagctcg gcatcgcgaa ggtgcggaac
      721 gattgcgggg taagaaagct gatttcgacg ccgaacgccc ttgccgcctc cgccatattg
      781 gtcagcgtcc gcaccacccc attgacctga ggatgccagg catcggtcgc gaccagaatg
      841 cgcatcaggc ggcccgcgcc gcgaccgagg tcacgggagc cgtccggcgc agcgggtcgg
      901 tccaggtgat gatttcaaac cggccgtctt cgtgctcggc gagcgcggtg cagctttcca
      961 cccagtcgcc gcagttcatg tagcggatgc catgctcgtc cctgatggtg gcgtaatgga
     1021 tgtggccgca gatcacccca tcggcgccat gacgccgcgc ctcggcggcc agagtctgtt
     1081 cgaaggcgcc gatataattc acggcgttct tgaccttcag cttggcccat tgcgacagcg
     1141 accaataggg cacgccgaac cagcgccgaa acgcgttgac gatgcgattc atctggatgg
     1201 cgaaatcgta ggccttgtcg ccaagatggg cgagccagcg cgcgttctgc accacgagat
     1261 cgaagatatc gccgtgaatc accagatagc gctgtccgtc gacccccaca tggatggtgt
     1321 tttccacgac gtcgatgccg ccgaaatgag tgccgtaata gttcctcagg aactcgtcat
     1381 gattgccagg cacatagaca attttggcgc ctttgcgcgc cttgcgcagc attttctgca
     1441 cgaagtcgtt gtgcgattgc ggccagtacc agctggattg cagcgcccag ccatcgacga
     1501 tatcaccgac gagatagatg gtatcggcat catggc
//
LOCUS       DASWGM010000986         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_403069,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000986 DASWGM010000000
VERSION     DASWGM010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403069"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VGA15_26550"
     CDS             complement(<1..400)
                     /locus_tag="VGA15_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX9591290.1"
                     /translation="MNGATAVLDIGKTNVKVATFACDGALVWERTMPNRMVPGPPYPH
                     ADVEMIWEFLLSALAEANKVNGIAAIVPTTHGCAAALIDDSGLVLPVMDYEFAGVEEI
                     EPFYAPLRPPFSQTFSPALPVGLNLARQLAW"
     gene            complement(444..761)
                     /gene="rhaM"
                     /locus_tag="VGA15_26555"
     CDS             complement(444..761)
                     /gene="rhaM"
                     /locus_tag="VGA15_26555"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEX9591291.1"
                     /translation="MTERYAFKMFLNPGCAAEYRRRHDAIWPELVCLLTKAGVANYSI
                     HLDQETSILFAYLERGEDHRMEVLPGHPVMRRWWDHMRDIMRTHPDGSPVAVPLTEIF
                     HLP"
     gene            complement(870..2003)
                     /locus_tag="VGA15_26560"
     CDS             complement(870..2003)
                     /locus_tag="VGA15_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX9591292.1"
                     /translation="MKQIMKNRMMKTAALVILGVAMAGGSAVAADKWDGGDDLPTSPL
                     ACDAGPAAAAAAKPYDGGQTTGAPDKAGKPITLVDVPKLIGIGYFNATSKGMQDAAKE
                     IGNVKVTTDGPTEAKIDEQIKFIDNYITRGVDGILFAANDPVAIAPVLKKALSKGIDV
                     VGYDANSQPDARQWFINQAQFNGIAKAMVDAMAKEAGEDKGFAIVTSTFTTPNQARWI
                     AEMSAYAAKCHPKLKWLETVEAQEDNVLSFNQATTLINKYGDKLSGLFGMTSVATPAS
                     AEAVTKAGLCGKLAVVGLATPNAMKPYVNAGCVKSVVLWNPVDLGYAATYVIRAVVDG
                     KLKPGATSVEAGKLGKLNVINGSEILLGSPFIFTKDNIKDFDF"
     gene            complement(2050..3030)
                     /locus_tag="VGA15_26565"
     CDS             complement(2050..3030)
                     /locus_tag="VGA15_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9591293.1"
                     /translation="MAVLAPEVRSAAPRWLIRHETLLAAILVVALAILGLFNERFLTI
                     DNLLNQGRLMTEVGLLALPMTFIIVTGGIDLSVGSIVGLCAIMLGYSWKNLGFPLPLA
                     IAFAIGVGGVAGFVNGLVVTRVRVPPLIMTIATLALYRGLAEGISQARSVRNYPEWFF
                     FTGQGEWLGVPVQLWILLAGIIVAGIALDRTTFGRTLYAVGNNETAARFSGLPVDRVK
                     LVVYTLSGLAAGLSAWVLVSRVTTTRSDMGSGYELDVIAAVVLGGTSIFGGVGTIWGT
                     VVGLVMIQLLKNGLALTGVKGDATIIVIGAVLIASTLIASSLHGRREVAG"
     gene            complement(3036..4016)
                     /locus_tag="VGA15_26570"
     CDS             complement(3036..4016)
                     /locus_tag="VGA15_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9591294.1"
                     /translation="MVVSRLRPGEGGALRLFASQEALLAFAIIGIAILVGLYNPRFLA
                     TRNLADIVLGNAYIAVAAIGMSMVIVSGNIDISVGSLIGVLATISGTLAVAGVPIILV
                     WLTPLVVGILVMMLQGAVIAYLRIPAIVVTLGMLSILKGGLISVTGGRWITDLPQSFH
                     LADIELFGILPMPVLIMVAATILAALWMRYSASGRAIYATGGNAEAARLCGVSPRRTV
                     VMVFALHGLFAGIAALLFATQLKVIQSTVPPNLELTVITAAVVGGVSILGGVGTVVGS
                     TLAAVLIAEIGSALVFINVSPYWIHAVQGALILVTVVADILRRRRRQVGG"
     gene            complement(4022..5578)
                     /locus_tag="VGA15_26575"
     CDS             complement(4022..5578)
                     /locus_tag="VGA15_26575"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX9591295.1"
                     /translation="MSEARNAPVREGGAAEPLLAVAGIEKSFPGVRALSGVSFEVAPG
                     EVHALLGENGAGKSTLIKIISGVYQPDAGTIHVDGRPLRLDSPDDARRAGIATIYQEL
                     LLFRDLTVAENVFMGHAPRSRGGRLDWKAMAAKTEALLAALEIVDLAPSRIVGSLSVG
                     NRQRVEILRALSQDARLLIMDEPTAALTEYDVTRLFDIVRRLKSRGVGIIYISHRMDE
                     IFQLADRVTVLRDGAQVGTRRVVETSSAELVQMMVGRQIESLFPKVAVAVGAPVLEVR
                     DLVRRPMTKSISLTVRAGEILGLAGLVGSGRSELAQTIFGVTPAESGRILVSGEPVQI
                     RSPGRARALGIAYVPEDRATQGLVRPMNVRENFSLASLARVARFGFIDSAAETRLAET
                     GVTRFRVRAGSLDQVVGRLSGGNQQKIVLGKWLANQPKLLILDEPTRGIDVGAKAEIH
                     RLMCELAAQGLAILMISSELPEVLGMSDRVLVMREGRLVAEFSRAEATPEAVGAAMMG
                     SQGADVGRAA"
     gene            complement(5575..>6099)
                     /locus_tag="VGA15_26580"
     CDS             complement(5575..>6099)
                     /locus_tag="VGA15_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX9591296.1"
                     /translation="ALCSDGDAIIINGGTTTFEMGEFLRERRLKVLTNSYPLAETLIH
                     DSNCRVALPGGEVYREQKLIVSPFEEDAIQHYSASRMFMSAISIGPLGVIEGDPLIAR
                     AETKLLKRAEKLVVLADSSKFTPRGSLVVCPLSRISTLITDAQAPKEALDMLRDAGVE
                     VMISTETPDEVAAA"
BASE COUNT         1143 a         2007 c         1922 g         1027 t
ORIGIN      
        1 gccacgccaa ttgccgggcg aggttcaggc ccacgggcaa ggcgggcgag aacgtttgcg
       61 aaaacggcgg gcgcagcggc gcataaaacg gctcgatctc ctcgacgccg gcgaactcgt
      121 agtccatcac cggcagcacc aggccggaat cgtcgatcag cgcggctgcg cagccgtggg
      181 tcgtcgggac aatggccgca atcccgttga ccttgttggc ttccgcaagg gcggagagca
      241 gaaactccca gatcatctcg acatccgcat ggggataagg cgggccgggc accatgcgat
      301 tgggcatggt gcgctcccac acgagcgcac cgtcgcaagc gaaggtcgcg accttcacat
      361 tggtcttgcc gatatcgaga accgcggtgg caccgttcat cctggattcc ccctcggacg
      421 gcgcgcatcg tccgatccga cgtctatggc agatgaaata tctcagtgag cgggaccgcg
      481 accggcgagc cgtcgggatg ggtgcgcatg atgtccctca tgtgatccca ccagcggcgc
      541 atcaccggat ggccgggcag tacctccatc cggtggtcct cgccgcgctc cagataggcg
      601 aacaggatcg aggtttcctg atcgagatga atcgaataat tggcgacgcc ggctttggtg
      661 agcaggcaca cgagttccgg ccagatcgcg tcatggcgcc ggcgatattc cgcggcgcat
      721 cccgggttaa ggaacatctt gaaggcgtag cgctcggtca tggtcgatcc gtaagccgag
      781 atctggaaat tcaagtagtg ccgattgcag gcgcaatcct cggccgcagc gccggcctct
      841 ccgccacgag aggccggcac gaaccgcgat cagaaatcga aatccttgat gttgtccttg
      901 gtgaagatga acggcgaacc caacagaatt tcgctgccgt tgatgacgtt gagcttgccg
      961 agcttgccgg cctcgaccga ggtcgcgccg ggtttcagct tgccgtcgac cacggcgcgg
     1021 atcacatagg tcgcggcata gccgagatcg accgggttcc acagcacgac ggacttgacg
     1081 caaccggcat tgacataggg cttcatcgca ttcggcgtgg cgagaccgac gacggcgagc
     1141 ttgccgcaca gaccagcctt ggtgacggct tcggccgagg cgggcgtggc gaccgacgtc
     1201 atgccgaaca ggccggaaag cttgtcgccg tatttgttga tcagcgtggt ggcctggttg
     1261 aacgacagca cgttgtcctc ctgcgcctcc accgtttcca gccatttcag tttgggatga
     1321 catttggcgg cgtaggccga catttcggcg atccagcgcg cctgattggg cgtggtgaac
     1381 gtgctggtga cgatggcaaa ccccttgtcc tcgccggctt ccttggccat cgcatcgacc
     1441 atcgccttgg cgatgccgtt gaactgggcc tgattgatga accactggcg cgcatcgggc
     1501 tgcgagttgg cgtcgtagcc gaccacgtca atccccttcg acaaggcctt cttcagaacc
     1561 ggcgcgattg ccaccggatc attggcggcg aacaggatgc cgtcgacgcc gcgggtgatg
     1621 taattgtcga tgaacttgat ctgctcgtcg atcttggcct cggtgggtcc gtcggtggtc
     1681 accttgacgt tgccgatctc cttggcggcg tcctgcatgc ccttggaggt tgcgttgaag
     1741 tagccgatgc cgatcagctt cggcacatcc accagcgtga tcggcttgcc ggccttgtcg
     1801 ggcgcgccgg tggtctggcc gccgtcataa ggtttggccg ccgccgcggc tgccgggccg
     1861 gcgtcgcatg caagcgggct ggtcggaaga tcgtcgccgc catcccactt gtcggcggcc
     1921 acggcagagc cgcccgccat cgccacgccc agaatcacga gcgcggccgt cttcatcatc
     1981 cgatttttca tgatctgctt cataatggtt tcctccctaa agcgccgctt tttgcggctt
     2041 atcaccccat cagcccgcga cctcgcggcg tccgtggaga gaacttgcga tcagcgtgga
     2101 cgcgatcaga accgcgccga tgacgatgat ggtggcgtcg cctttgactc cagtcagcgc
     2161 cagcccgttc ttcaacagct ggatcatgac caggccgacg acggttcccc agatcgtccc
     2221 aaccccgccg aaaatgctgg tgccgccgag aaccacggcg gcgatgacgt cgagttcgta
     2281 gccgcttccc atgtccgagc gcgtcgtggt cacgcgggaa accagcaccc aggccgacag
     2341 ccccgcggcc aatccggaca gcgtgtagac cacgagcttg acccgatcga ccggcaggcc
     2401 ggaaaaccgg gcggcggtct cgttgttgcc gacggcatag agcgtgcggc cgaaggtggt
     2461 gcggtccagc gctatgccgg ccacgatgat ccccgccagc aggatccaga gctgcaccgg
     2521 cactccgagc cattcccctt gcccggtgaa gaagaaccat tcgggatagt tgcgcaccga
     2581 ccgcgcctga ctgatgcctt cggcgagccc ccgatagagc gccaaggtcg cgatggtcat
     2641 gatcagcggc ggcacccgga cacgggtgac caccaacccg ttgacaaagc cggcgacgcc
     2701 tccgaccccg atcgcgaagg cgatcgccag cggcaacggg aagccaaggt ttttccagga
     2761 atagccgagc atgatcgcgc acagcccgac gatcgatccc accgacaagt cgatgccgcc
     2821 ggtgacgatg atgaacgtca tcggcaaagc gagcagacct acctcggtca tcaggcggcc
     2881 ctggttcagc agattgtcga tggtcaggaa gcgctcgttg aataggccga ggatcgccag
     2941 cgcgacgacc agaatcgccg ccagcagcgt ctcgtggcgg atcagccaac gcggcgcggc
     3001 ggatcggacc tcgggtgcga gcaccgccat gcgcattatc ctcccacctg acggcgccgg
     3061 cgccgcagga tgtcggcgac caccgtgacc aggatcaacg caccctgcac ggcgtgaatc
     3121 cagtacggcg agacgttgat gaagaccagc gcgctgccga tttcggcgat caagaccgcc
     3181 gccagcgtcg atccaacaac ggtgccgaca ccgccgagga tgctgacgcc gccgaccacc
     3241 gcggcggtga tcaccgtcag ctccaggttg ggcggtaccg tcgactggat caccttgagc
     3301 tgggtcgcga acagcagcgc cgcgatccct gcgaacagcc catgcagcgc gaataccatc
     3361 accaccgtgc gccgcggcga cacgccacac agccgcgcgg cttcggcatt gccgccggtg
     3421 gcatagatcg cgcgaccgct tgccgaatag cgcatccaca gcgcggccag aatggtcgcc
     3481 gccaccatga tcagcaccgg catcggcaaa atcccgaaca attcgatatc ggcgagatgg
     3541 aagctttgcg gcaggtcggt gatccatcga ccgcccgtga cgctgatcag gccgcccttg
     3601 aggatcgaca acatccccag cgtaaccacg atcgccggaa tacgtaagta ggcgatcacc
     3661 gcgccttgca gcatcatgac gaggatgccg accacgagcg gcgtgagcca gaccagaatg
     3721 atcggcacgc ccgcgaccgc tagcgttccg ctgatagtgg cgagaacgcc gatcagggag
     3781 ccgacagaaa tatcgatatt gccggacacg atcaccatcg acatgccgat cgccgccacc
     3841 gcgatatagg cgttgccgag cacgatgtcg gcgaggtttc gcgtcgccag aaagcgcggg
     3901 ttgtagaggc ccaccaggat cgcaatgccg atgatcgcga aagccagcaa ggcctcctgc
     3961 gaggcgaaca agcgaagcgc gccgccttcc ccgggacgca ggcgcgaaac caccatggtg
     4021 ctcacgccgc cctccccaca tctgcgccct ggctgcccat cattgccgcg ccgaccgctt
     4081 caggcgtggc ttccgcgcgc gaaaattcgg cgaccagccg gccctcgcgc atcaccagta
     4141 cgcggtcgct catgccgagc acttcgggca actcgctgga gatcatcagg atggccaagc
     4201 cttgtgcagc gagttcgcac atcagccggt ggatttcagc cttggcgccg acgtcgatgc
     4261 cgcgggtcgg ctcatcgaga atgagcagct tcggctggtt ggcgagccac ttgcccagca
     4321 cgatcttctg ctggttgcct ccggacaatc ggccgaccac ctggtcgagc gaacccgccc
     4381 tgacgcgaaa gcgggtgacc cccgtttcgg ccaatcgcgt ttctgcggcg gaatcgatga
     4441 agccgaagcg cgccactctg gccagactgg ccagggaaaa attctcgcgc acattcatgg
     4501 ggcgaaccag gccctgggtg gcgcggtctt cggggacata ggcgatgcca agcgcccgcg
     4561 cccggccagg cgatcggatt tgcaccggct cgcccgaaac caggatccgg ccggattcgg
     4621 ccggcgtcac gccaaagatg gtctgcgcca attcgctgcg cccggagccg accaggccgg
     4681 cgaggcctag gatttcgccg gcacgcacgg tcaggctgat actcttcgtc atcggccgcc
     4741 gtaccagatc ccgcacttcc agcaccggcg cgccgaccgc cacggcgacc ttggggaaca
     4801 ggctttcgat ttggcggccg accatcatct ggaccagttc cgccgatgac gtctccacga
     4861 ctcggcgggt gccgacctgg gcaccatcgc gcagcaccgt cacccgatcg gccaattgga
     4921 agatctcgtc catgcggtgg ctgatataga tgatgcccac accgcgcgat ttgaggcgcc
     4981 gcacgatgtc gaacagccgc gtcacgtcat attcggtcaa cgcggcggtc ggctcgtcca
     5041 tgatcaagag ccgcgcatcc tgtgacagcg cgcgcaagat ctcgacgcgc tggcgattgc
     5101 cgacgctgag cgagccgacg atccggctcg gggccagatc gacaatttcg agggccgcga
     5161 gcagcgcctc ggtcttggcc gccatcgcct tccagtcgag ccggccgccg cggctgcgcg
     5221 gcgcgtggcc catgaagacg ttttcggcca ccgtcagatc gcgaaacagc aacagttcct
     5281 ggtagatggt cgcgatcccg gcgcgccgcg cgtcatccgg agaatcgagc cgaagcggcc
     5341 gtccgtcgac atgaatggtg ccggcgtcgg gctgatagac cccggagatg atcttgatca
     5401 gggtggattt gccggcgccg ttctcgccta gcaaggcgtg cacttcgccc ggcgcgacct
     5461 cgaacgagac ccccgacagc gcgcgaaccc cggggaagga tttttcgatg ccggccaccg
     5521 ccagcagcgg ctccgctgca ccgccctctc gcacgggtgc gttgcgggcc tcgctcatgc
     5581 ggccgcgacc tcgtcgggag tctcggtcga gatcatcacc tcgacgccag cgtcgcgaag
     5641 catgtcgagt gcttccttcg gggcctgggc gtcggtgatc aaggtcgaaa ttcgcgacag
     5701 cggacaaacc accaggctgc cgcgcggcgt gaatttggag ctgtcggcca gcaccaccag
     5761 cttctcggcg cgcttgagta gcttggtctc ggcgcgggcg atcaagggat cgccctcgat
     5821 cacgccaagc gggccgatcg aaatcgcgct catgaacatg cgcgaggccg aataatgctg
     5881 gatcgcgtcc tcctcgaacg gcgagacgat cagtttctgt tcgcggtaga cctcgccgcc
     5941 tggcagcgcg acgcgacagt tcgaatcgtg gatcaacgtc tcggccaatg gataggaatt
     6001 ggtgagcacc ttgaggcggc gctcgcgcag gaattcgccc atctcgaagg tcgtggtgcc
     6061 gccattgatg atgatggcgt cgccgtcgct gcacagcgc
//
LOCUS       DASWGM010000987         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_663570,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000987 DASWGM010000000
VERSION     DASWGM010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663570"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /gene="dxr"
                     /locus_tag="VGA15_26585"
     CDS             complement(<1..1004)
                     /gene="dxr"
                     /locus_tag="VGA15_26585"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX9591297.1"
                     /translation="MSAVPLRNNKAAASAVRAVTVLGATGSIGDSTMDLLRASRDRYQ
                     VEALTANSNVQGLAKLAREFGARFAAVADPDRLTELKDALAGTRTECGAGESAIIEAA
                     ARPADWVMAAVSGAAGLKPALAAVDRGATVALANKECLVCAGDFFMQRAAKAGACILP
                     ADSEHNALFQALGSGNREELVRVIITASGGPFRTWASADIEQATLAQALKHPNWSMGQ
                     KITIDSASMMNKGLEVIEASYLFALTPDEIDVLVHPQSIIHGMVEFSDCSVVAQLGAP
                     DMRTPIAHCLGWPDRIVGPAAKLDLAKIGQLTFEAPDFGRFPGLRLAYDALRTGRGAT
                     T"
     gene            complement(1009..1857)
                     /locus_tag="VGA15_26590"
     CDS             complement(1009..1857)
                     /locus_tag="VGA15_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX9591298.1"
                     /translation="MTEGDAAPAAVVEQGSRNLLMRVIAALVLAPSAIAIAYAGGWLW
                     TALVTLAAIGLYVEWLMVVGAVRQMRVVASGVVALAIAGFCLASGWIDAALLVLALGF
                     AAVALLSPERRGWTAAGFFYAGSAEMASILVRLDQVYGFVALILILLVVWVTDIGGYF
                     AGRGVGGPKLWPRVSPKKTWAGAIGGFVASLFVAAGLAAFGLGKTGPLVLLGAVLSVA
                     SQLGDLFESAVKRRFGVKDSSHIIPGHGGLLDRLDGFVAAVVLATIFGFLRGGADGVG
                     RGLVVW"
     gene            complement(1854..2612)
                     /locus_tag="VGA15_26595"
     CDS             complement(1854..2612)
                     /locus_tag="VGA15_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX9591299.1"
                     /translation="MSNAAAPATEGPDRTDGPLHVAIIMDGNGRWAAARGLPRAEGHR
                     RGVEALRRVVRAANELGILYLTIFSFSSENWSRPATEIGDLFGLLRRFIRNDLATLHR
                     DGVRVRVIGERDGLEPDICALLNEAEELTRGNTRLHLVVAFNYGSRQEIAHAAQRLAR
                     EVADGKRDPASIDADTLGRYLDAPDIPDPDLIIRTSGEQRLSNFLMWQAAYSELVFVP
                     IHWPDFDKVALEGAIAEYARRERRFGGLAAKTGS"
     gene            complement(2644..3207)
                     /gene="frr"
                     /locus_tag="VGA15_26600"
     CDS             complement(2644..3207)
                     /gene="frr"
                     /locus_tag="VGA15_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX9591300.1"
                     /translation="MPTGSFDINELKRRMQGATQSLKHELGGLRTGRAAASMLEPVQV
                     DAYGTHMPLNQLATISVPEPRLLSVQVWDKSMVKAVEKAIVDSNLGLSPATEGQVLRL
                     RIPELNEERRKELVKVAHKYAEAAKVAVRHVRRDGLDTVKKLEKNHEISEDDQERLAN
                     DVQKATDGTISEIDQLLAAKEKEILTV"
BASE COUNT          646 a         1115 c          955 g          569 t
ORIGIN      
        1 gtggtcgcgc cgcggccggt ccgcagcgcg tcatacgcaa gccgaagccc gggaaagcga
       61 ccgaaatccg gcgcttcgaa agtcagttgt ccgatcttgg caagatcgag cttcgctgcc
      121 gggccgacga tgcgatcggg ccatccgagg caatgcgcga tcggcgtgcg catgtcgggc
      181 gcccccaact gcgcgacaac ggaacaatcg gaaaattcga ccatgccgtg gatgatcgac
      241 tgcgggtgca ccagaacgtc gatctcatcc ggcgtcagcg caaacaagta agaggcttca
      301 atgacttcca gccccttgtt catcatcgag gcggaatcga tggtgatttt ctgtcccatg
      361 ctccagttcg gatgtttcaa cgcctgcgcc agcgtcgcct gctcgatatc ggcgctggcc
      421 caggtccgga acggtccgcc cgacgcggtg ataatgacac gaaccagctc ttcccgattg
      481 ccggaaccca gcgcctgaaa cagcgcgtta tgctcggagt ccgccggcag gatgcaggcc
      541 ccagccttcg ccgcccgctg catgaagaaa tcgcctgcgc aaacaaggca ttccttgttg
      601 gcgagtgcta ccgtcgcgcc gcgatccacg gccgcaagcg caggtttcag tccggccgca
      661 ccgctgaccg cggccatgac ccaatccgcc gggcgcgccg ctgcctcgat tattgcgctt
      721 tcgccggcac cgcattcggt gcgcgtgccc gccagcgcat ctttcagctc ggtgaggcga
      781 tcgggatcag cgaccgccgc aaatcgcgca ccaaattccc tcgccagctt cgccaacccc
      841 tgcacattgg agttggcggt cagcgcttcg acctggtagc ggtcgcgcga ggccctcagc
      901 aaatccatcg tgctgtcgcc gatcgaaccg gtggcgccga gaacggtgac ggcgcgtacg
      961 gcagatgccg cggctttgtt gttacgcaag ggaacggcgc tcataaattc accaaaccac
     1021 aagaccgcgg ccgacgccat ccgcgccacc ccgcaaaaac ccgaaaatcg tcgccaggac
     1081 caccgctgcg acgaacccgt caagccggtc cagtaacccg ccatgccccg gaattatgtg
     1141 acttgagtcc ttgacgccga atcgccgttt cactgcggat tcaaagagat cgccgagttg
     1201 ggaagcaacc gagagaaccg cgcccagtag caccaatggg ccggttttgc cgagaccgaa
     1261 cgccgcaagc cccgcggcga cgaaaaggct cgctacgaaa ccgccgatgg cgcctgccca
     1321 ggtcttcttg ggactgaccc gcggccagag ttttgggccg ccaactccgc gacctgcaaa
     1381 ataaccgccg atatcggtca cccagaccac cagaaggatc agaatcagcg cgacaaatcc
     1441 gtaaacttgg tcgaggcgca ccaagatcga tgccatctcc gctgagcctg catagaaaaa
     1501 tcccgccgcg gtccagccgc gccgctccgg cgaaagcagc gcaacggcag cgaagccgag
     1561 ggccaaaacg aggagcgccg catcgatcca cccggatgcg agacaaaatc cggcaatggc
     1621 aagcgcaaca acacctgacg cgaccacgcg catctggcgc accgcgccca caaccatcag
     1681 ccattcgacg taaagaccga tcgcagccag cgtcactaac gcggtccaca accatccgcc
     1741 ggcataagcg atcgcaatcg ctgaaggtgc gagcaccaac gcggcgatga cgcgcatcaa
     1801 cagattgcgc gaaccctgct cgacaacagc cgccggcgcg gcgtcgccct cggtcatgat
     1861 ccggttttcg cggctagacc gccgaaacgg cgctcccgcc tggcgtattc ggcgatcgcg
     1921 ccctcgagcg cgaccttgtc gaaatccggc cagtggatcg gcacgaacac gagctcgctg
     1981 taggccgcct gccacatcag gaaattcgac agccgctgct ctccgctggt gcgaatgatc
     2041 aaatccggat cgggaatatc gggcgcatcg agatatcgac caagcgtgtc ggcatcgatc
     2101 gacgcggggt cccgcttgcc gtccgcgact tcgcgcgcca gccgctgcgc cgcatgagcg
     2161 atttcctgcc gcgatccata gttgaacgcg acgacgagat gaagcctggt attgcccctg
     2221 gtcagttctt cggcttcgtt cagaagcgcg cagatgtccg gctccagtcc gtcgcgctcg
     2281 ccgatcaccc gcacccggac gccatcgcga tgcagggtcg ccagatcgtt gcggatgaag
     2341 cggcgcagca ggccgaacag atcgccgatt tcggtcgccg gccgcgacca gttttccgag
     2401 ctgaaggaaa aaatcgtcag gtacaggatg ccgagttcgt tggcggcgcg aacgacgcgg
     2461 cgcaaggcct cgacgccccg gcgatgacct tccgcgcgcg gcagaccccg ggccgctgcc
     2521 cagcgcccgt ttccgtccat gatgatcgcc acatgcaagg gaccgtcggt tcgatccggt
     2581 ccttcagttg cgggggcggc ggcgttcgac atccatcaat cccttgacct agcaacccct
     2641 gacctaaacg gtgaggattt ccttttcctt ggccgcaagc aactgatcga tctccgaaat
     2701 cgttccgtcg gttgccttct gcacatcatt ggccagccgt tcctgatcgt cctcggaaat
     2761 ctcgtggttt ttctcaagtt tcttgacggt atcgaggcca tcgcggcgga catgccggac
     2821 cgcgactttt gccgcctcgg catatttatg cgccaccttc accagttcct tgcggcgctc
     2881 ctcgttgagt tcgggaattc tcaggcggag cacctgccct tcggtggcgg ggcttaagcc
     2941 gaggttggag tccacgatcg ctttctccac cgccttgacc atcgacttgt cccatacctg
     3001 cacggacagc aggcgtggct ccggcacgct gatggtcgcg agctggttaa gcggcatgtg
     3061 ggtgccgtag gcatcgacct gcaccggctc cagcatcgag gccgcggcgc ggccggttcg
     3121 cagaccgccc agctcatgct tgagcgactg ggtggcgccc tgcatgcgcc gcttcaattc
     3181 gttgatgtcg aaactacccg taggcataac actctccctc ctgaaagctc gtcgccaggg
     3241 ccttcatccc gcgcatcggt gcttcgcatg tcaaccggcg acgat
//
LOCUS       DASWGM010000988         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_528275,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000988 DASWGM010000000
VERSION     DASWGM010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528275"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VGA15_26605"
     CDS             <1..282
                     /locus_tag="VGA15_26605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9591301.1"
                     /translation="KVAPAQLEALGKWGIQFDRAYDYLKSIRQPTLVVNGGNDVIVYS
                     VNSFILQQHLPNAQLILYPDANHGSQYQYPELFVRHVSMFLSADERRGV"
     gene            334..1080
                     /locus_tag="VGA15_26610"
     CDS             334..1080
                     /locus_tag="VGA15_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9591302.1"
                     /translation="MAILAGKTALVTGASRGIGRAGALALAAAGAQVLVHYGRGAKEA
                     EDVVAEIRKAGGRADAITADLAAADGAGKLARQARSIVGDRLDILVTNAGVLKAATIE
                     ETTVEDFDKLFAVNVRAPFFLVQQLLPIMCKGSSIVFLSSLAARAVVGTEPAYAATKG
                     AVDTLVKHFASMLGARGIRVNAVAPGVVETDMLNFTKTGAGRDFALGIQALKRLAQPD
                     DIGGVIAFLASEDARWITGDIIRVDGGSNL"
     gene            1237..2088
                     /locus_tag="VGA15_26615"
     CDS             1237..2088
                     /locus_tag="VGA15_26615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX9591303.1"
                     /translation="MVAHTLWTSAAPAMTYPLFAEEWHLTHTVTTGVFAIYPIVVAAV
                     LVGFGDVSDYVGRRAAILWGVGASLFGTLLFALAPDVLWLFAGRAFMGIGVGLTAGPS
                     TAAMVEFSGQGAAKRAATITTAAQAVGFAAALLLGGALIQYAPLPTRLNFWVLAAVLA
                     ALFAAAWFLPRHTGSEALGRWRPKTPFIPKRLRNVFAVAAAAVTSAYTHGLLILSLGS
                     QVARDLVGSPNALVNGAALSLFAIASAVVGIGARRLQPRTAMTGGAAASALVQRFLDL
                     THIRRPF"
     gene            complement(2072..>2281)
                     /locus_tag="VGA15_26620"
     CDS             complement(2072..>2281)
                     /locus_tag="VGA15_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018117072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX9591304.1"
                     /translation="GRFEFTFTPKHGSWLNLVEGFFSKFARSVLRHIRVASKQELKDR
                     IMAAMDHFNENTVVHTWSYKLKKAA"
BASE COUNT          375 a          733 c          716 g          457 t
ORIGIN      
        1 aaggtggcgc ccgcccaact cgaagccttg ggcaaatggg gcattcagtt cgatagggcg
       61 tacgactacc tcaagtcgat tcgacagccg acgctggtgg tgaacggcgg caacgacgtc
      121 atcgtctact ccgtgaactc gttcatcctg cagcagcacc tgccgaacgc ccaactcatc
      181 ctctaccccg acgccaatca cggctcgcag taccagtatc ccgaactctt cgtccgtcat
      241 gtctcgatgt tcctgtcggc ggacgagcgc cgaggcgtgt gaggatcgaa agcggtcacg
      301 ctatccgccg cagcaactac aaggacgatg atcatggcta ttttggccgg caaaaccgct
      361 ctcgttaccg gcgcttcccg cggcatagga cgcgccggcg cactcgcgct cgctgccgcc
      421 ggcgcccagg ttctcgtgca ttacggccgg ggagcgaagg aagccgaaga tgttgtcgca
      481 gagattcgca aagccggtgg ccgggcggat gccataacgg ccgacctcgc cgccgcggac
      541 ggcgccggca agcttgccag gcaggcccgc agcattgtcg gagatcgcct ggacatcctt
      601 gtcaccaatg caggggtctt gaaagcggcg accatcgagg aaacgacggt cgaggatttc
      661 gacaagctgt tcgcagtcaa cgtccgcgca ccgttctttc ttgtgcagca gctgcttccg
      721 atcatgtgca agggaagcag catcgttttc ctctcatcgc tcgcggcgcg cgccgttgtt
      781 ggaacggaac ccgcgtatgc ggcaaccaag ggtgccgtcg acaccctggt gaagcatttc
      841 gcctcgatgc ttggcgcacg cggtatccgc gtcaatgccg tcgcgcccgg tgtcgtcgag
      901 acggacatgt tgaacttcac caagacaggc gcgggccgcg acttcgcgct gggcattcag
      961 gcgctgaagc gcctcgcgca gcccgacgac atcggcggcg ttatcgcctt cctggcatcc
     1021 gaagatgccc gatggatcac cggcgatatc atccgcgtcg atggcggctc gaacctctga
     1081 agttggaacg ccgggagcgc cgtgagtaaa gcgatgatca agggtcccct tctgtctcca
     1141 gccgcttgcg ggcctcaccg gcccggctgc tgtacccagc ggaactccgc accgtacgag
     1201 cttgggcaac gcacaagctt ctgggttgcg gccggcgtgg tcgcgcacac cttgtggacc
     1261 agcgctgcgc cggcgatgac ctatccgctg tttgccgagg agtggcatct cacccacacg
     1321 gttacgaccg gggtcttcgc catctatcct attgtcgtgg ccgccgttct ggtcggcttc
     1381 ggcgatgtgt ccgattatgt cggccgtcgc gccgcaatcc tgtggggtgt cggcgcttcg
     1441 ctctttggca ccctgctgtt cgcgctggcg cccgatgtgc tctggctgtt tgcggggcgc
     1501 gccttcatgg gcatcggcgt cggcctcact gcgggcccgt cgaccgccgc gatggtggaa
     1561 ttcagcggcc agggagcggc aaagcgcgcc gctacgatca caaccgccgc ccaagccgtc
     1621 ggttttgccg cggccctgct gctcggcggt gccctcattc aatatgctcc gctgcccacg
     1681 cggctgaatt tttgggtgct ggccgcggtc ttggccgcgc tgtttgccgc ggcatggttt
     1741 ctgccgcgcc ataccggcag cgaagccctg ggccgctggc gtcccaaaac ccccttcata
     1801 ccgaaacgtt tgcgcaacgt tttcgcggtc gcggcggcgg cggtaacctc tgcctatacg
     1861 catggcttac tgatcctgtc gctcggaagt caggttgccc gcgatctcgt tggatcgccc
     1921 aacgcgctcg tgaacggcgc cgctctttcc ctattcgcga tcgcctcggc cgtggtcggg
     1981 attggcgcga ggaggctgca acctcggacc gcgatgaccg gtggcgccgc cgcatccgca
     2041 ctagttcagc gtttccttga tctgactcat atcaggcggc ctttttgagc ttgtaggacc
     2101 aagtgtgaac gaccgtgttc tcgttgaagt gatccatggc agccatgatg cggtccttga
     2161 gttcttgttt ggatgcgacg cggatgtggc gcaggacgga gcgcgcgaac ttggagaaga
     2221 agccttcgac caggttcagc caggagccgt gcttgggcgt gaaggtgaac tcgaagcgac
     2281 c
//
LOCUS       DASWGM010000989         2790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_403998,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000989 DASWGM010000000
VERSION     DASWGM010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403998"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1049)
                     /locus_tag="VGA15_26625"
     CDS             complement(201..1049)
                     /locus_tag="VGA15_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591305.1"
                     /translation="MINTLRRASTLWASMATVIFAGLAPAFSQTPTDAQRSAIRSECR
                     SDYEVHCASVPPGGAASLQCLQKNMSSLSSGCQTAVRAVEPPAAPKAESAPAAAPKTE
                     SKIESKTESATAKPAETAAPATPKAATDAPAKRPSSAQVAAIRSACRSDYQKVCAGVP
                     TGGAPALQCLEKNKSKVSPACEQAVNAASGGTAAPAAGGAPTTAATASPDAAPAAAPA
                     PALVLRPMRPREELFVLRSACGGDVRALCGGIPVGGGRIARCLATQAASLSPACKEVL
                     GQFAAR"
     gene            1282..2484
                     /locus_tag="VGA15_26630"
     CDS             1282..2484
                     /locus_tag="VGA15_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9591306.1"
                     /translation="MKPTRSPAFSGWDVAARQTAANACRLYFAIAAFVVLTGCEQNSF
                     VPPPPPKVDVGLPVQQSVTRFLEATGNTAPIKSVDLVARVQGVLQSIDYKDGTFVKEG
                     ATLFTIEPETYKLKLEQAEAAEAGAQASVRQAEADFKRQSDLVSRQAVSQATLDQSTS
                     SRDNAQANLQQAQANTKIAAVNFGYTNVVAPFDGIVSAHLISVGELVGAASPTQLATI
                     VQLDPIYVNFNVNEQDVLRIRAEAKRRGLTASDLTQTPIEIGLQTEDGYPHRGKLDYA
                     APTINQSTGTLAVRGVLSNAERVLLPGYYVRVRVPFGEQQNALLVPDVALGSDQAGRY
                     VLVVNAEDVVEQRKVQTGPAESDLRVIESGLKPDDRVVIAGLLRAIPGQKVDPQLQKI
                     EAPPAPAK"
     gene            2493..>2790
                     /locus_tag="VGA15_26635"
     CDS             2493..>2790
                     /locus_tag="VGA15_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9591307.1"
                     /translation="MISKFFIERPVLSNVIAILMILIGGVSLFSLAVAQYPDVVPPTV
                     QVTTRYPGSSAKTVIDTVALPIEQQVNGVEDMLYMQSYSGADGTYSLTVTFKIGT"
BASE COUNT          524 a          867 c          863 g          536 t
ORIGIN      
        1 gatcctcctg gtcctgaatt tcctcgacgg tttttataag cttgcaaggc tggagtggca
       61 tgccgcgtac cagatcgtca tgtcattctg ctacattgtc ttgtcggtcc gattgatcca
      121 gttgatcctc gcctttcgcg gcgagtagcc ggcggatctc gcgacgattc gcgagactgc
      181 cggcagagca ggcgcatggt ttatcgcgct gcgaactggc ccagcacttc cttgcacgcg
      241 ggcgaaagcg aggctgcctg cgtggccaga catcgcgcga tacggccacc gccgaccgga
      301 atgcccccgc aaagcgcgcg cacatctccg ccacatgccg accgcaagac gaacaactcc
      361 tcgcgcggcc gcatcggccg caacaccagc gcaggcgccg gcgcggcggc gggagctgcg
      421 tcaggactgg cggtcgcagc cgttgtcgga gcgccgcctg ctgcgggcgc cgcagtgccg
      481 ccgctggcgg cgttgaccgc ctgctcgcag gctggcgaga ctttcgactt gttcttctcc
      541 agacattgca gcgccggggc gcccccggtc ggcacgccgg cgcagacctt ttgatagtcg
      601 gagcggcatg cactgcgaat ggcagcaacc tgtgcgctgc ttggcctttt ggccggtgcg
      661 tctgtggcgg ccttgggagt cgccggcgcc gcggtttcgg ctggtttggc cgttgcggac
      721 tcggtcttgg actcgatctt ggactcggtc ttgggcgccg cggccggtgc cgactcggcc
      781 ttgggcgcag ccggaggctc taccgcgcgc acggcggtct gacagcctga cgagaggctc
      841 gacatgtttt tctgcaggca ttgcagggac gccgcgccgc ccggcgggac actggcgcaa
      901 tggacttcat agtcggagcg gcattcggat cggatggcgc tgcgttgcgc atcagtcggg
      961 gtttgcgaaa atgccggcgc caaacctgcg aatatgacgg ttgccatcga cgcccacagc
     1021 gtcgacgcac gtctcaacgt gttgatcatt ttatgatatc cttatcgttt gcagtgcggc
     1081 agcggcttcg gcgctgcagc agcggcttca atcgaagtga aatcttctaa aaaatgtcga
     1141 cagaaaaact ttcgcaggcg atatcatttt ctctttcgaa tcctaccgca agtagattgt
     1201 ttgccatttt catggggtag ccgaaagcgc gttcttctga ataaaaaact tttcgagaac
     1261 caaacggatt ggggtacggg aatgaagccg acgcgatcgc cagcattcag cggatgggat
     1321 gtcgcggcgc ggcaaacagc cgcgaacgcc tgccgtctgt acttcgccat cgcggcgttc
     1381 gtcgtgctga ctggctgtga gcaaaatagt tttgtgccgc cgccgccgcc gaaggtggat
     1441 gtcggattgc cggtgcagca atccgtcacg cgctttctcg aagccaccgg caacaccgcc
     1501 ccgatcaaat cagtcgatct cgtcgcgcgc gtgcagggcg tgctgcaatc gatcgactac
     1561 aaggacggca ccttcgtgaa agagggtgcg acgctgttca cgatcgagcc cgaaacctac
     1621 aagctgaaat tggagcaagc cgaggcggcc gaggccggcg cgcaggcgtc ggtcaggcag
     1681 gccgaagccg atttcaaacg ccagtcggat ctggtttcaa gacaagccgt ttcgcaggca
     1741 acgttggatc agtccacttc ttcgcgcgac aacgcccagg ccaacctgca gcaggcccag
     1801 gccaatacca agattgcggc ggtcaatttt ggctacacca atgtcgtcgc gcccttcgac
     1861 ggtatcgtca gcgcgcatct gatctcggtc ggcgaactgg tcggcgcggc gtcaccgacc
     1921 cagctcgcca ccatcgtgca gctggacccg atctacgtca atttcaacgt caacgaacag
     1981 gatgtcctgc gcattcgcgc ggaagccaaa cggcgcggat tgacggcgag cgatctcacg
     2041 caaacgccga tcgagatcgg actgcagacc gaagacggct atccgcacag gggcaagctc
     2101 gactatgcgg cgccgacgat caaccagtcg acgggaacgc tcgcggtgcg cggcgttctg
     2161 tcgaatgccg agcgcgtctt gctgccggga tattatgttc gtgtccgcgt tcccttcggc
     2221 gagcagcaga acgccttact cgtccccgat gtcgcattgg gcagcgatca ggccggacgc
     2281 tacgtactgg tggtcaacgc cgaagacgtc gtcgagcagc gcaaggtcca gaccgggccg
     2341 gccgagagcg atttgcgcgt gatcgaaagc ggcttgaagc cggatgatcg tgtcgtcatc
     2401 gccgggctat tgcgcgcgat ccccggccag aaggtcgatc cgcaactgca gaagatcgaa
     2461 gcgccaccgg caccagccaa ataggaccgg ccatgatttc gaaattcttc atcgaacggc
     2521 cggtgctctc caacgtcatc gcgatcctga tgatcctgat cggcggcgtt tcgttgttca
     2581 gccttgcggt cgcgcaatac cctgacgtgg tgccgccgac cgtgcaggtc acgacacgct
     2641 atcccggctc cagcgccaag accgtcatcg ataccgtggc actgccgatc gagcagcagg
     2701 tcaacggcgt cgaggacatg ctctacatgc agtcctacag cggcgccgac ggcacctatt
     2761 cgctcacggt tactttcaag atcggcactg
//
LOCUS       DASWGM010000990         8317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2214418,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000990 DASWGM010000000
VERSION     DASWGM010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214418"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VGA15_26640"
     CDS             complement(<1..834)
                     /locus_tag="VGA15_26640"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9591308.1"
                     /translation="MGRKIAIVGAGAVGGYAGAHMVQAGEDVTFIDPWPEHIEHMRKH
                     GLRVTHARDVAEFSVPVRALHVTDAQQLAKETPVDIAFVCMKSYDTAWATTLIQQYLA
                     PDGYVVSLQNCMNEETIAGIVGWGKTLGCIASSITVNLPEPGHIHRGAGKGGTAHTVF
                     RAGEVHGRITRRAEEICRLVGYADSAKVTGNLWGERWSKLVANAMGNGLSACTGLPGG
                     QMLQSEPIRRFSTRLGSEAIRVGQAHGYQLEEVLHLPPETIARAGEGDEAAMRACDEQ
                     RF"
     gene            complement(1033..1350)
                     /locus_tag="VGA15_26645"
     CDS             complement(1033..1350)
                     /locus_tag="VGA15_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX9591309.1"
                     /translation="MTGRRPFSELTKDFTPARRARVAAKAAALREAMTLEELRKARSL
                     SQAEVADALTVNQPAVAKLEKRGDMHVSSLRRYIEALGGTLEITAHFDKTTVVISNIG
                     ETP"
     gene            complement(1347..1709)
                     /locus_tag="VGA15_26650"
     CDS             complement(1347..1709)
                     /locus_tag="VGA15_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX9591310.1"
                     /translation="MAWNVEHTDEFAEWYHGLSEAQQDDITAAGLLLIEQGPQLPFPY
                     SSGINGSKHAHMRELRVQSGGRPLRVFYAFDPRRSAILLIGGDKTGDDRFYERMVPIA
                     DQLYDIYIAEIRKEGLIP"
     gene            complement(1811..2809)
                     /locus_tag="VGA15_26655"
     CDS             complement(1811..2809)
                     /locus_tag="VGA15_26655"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX9591311.1"
                     /translation="MQLRVFGRTGMQLSVLGFGCGAVGGFMVRGDPADQERTIARAVA
                     AGVNYFDTAVQYGNGESENNLGRVLQKLKPAGVVVGTKVRLPPGEFGRVADAVTMSLE
                     GSLARLGLDRVDIFHLHNPITETGGGSALSVRQVLDDVVPAFERLRAQGKIRFLGMTA
                     VGDTAALQQVINARAFDSAQIVYNMLNPSAAEALPTNYPAQDYGRLFDSTRAAGVGVV
                     GIRVLAGGALSGSAERHPIAGPAPEPIGSAMSYDADVDRARRLIPLVGEGFAASLTEA
                     ATRFALSQPAMGTILVGMATPQQFEDALAAVQKGPLPQAALDRLSALRQEFSGEPR"
     gene            complement(3020..3553)
                     /locus_tag="VGA15_26660"
     CDS             complement(3020..3553)
                     /locus_tag="VGA15_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX9591312.1"
                     /translation="MFTSRCAIFSAALLSAMMTDASLAETPNLGKPIDETAIAAWDIS
                     ILPDGAGLPKGSGTPAQGAVVYADKCALCHGDNGKGGSAAALVSDREIAGISAAQKTI
                     KNFWPYSTTIFDFIRRAMPFQTPRSLTDDEVYALTAYILAENKLIGANDMMNAQTLPK
                     VKMPNRENFIIRFPDRI"
     gene            complement(3540..4865)
                     /gene="soxC"
                     /locus_tag="VGA15_26665"
     CDS             complement(3540..4865)
                     /gene="soxC"
                     /locus_tag="VGA15_26665"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEX9591313.1"
                     /translation="MANARHRNARGQTLEAAAGNGLIDRRALLGRGMVFAGAMGAGIG
                     PSSAAAGAEPLADDPWSMEVGDPMPLYQVPSKFETKVARTLSNPNHEPRNSHARTPHH
                     LLEGTTTPNGLHFAINHAGVPNIDPEKHRLVIHGLVKRPLEFTLETLSRYPMISRVSF
                     VECGGNSAPMFSPEPMQETVQALHGLASCAEWTGIKLSTLLEETGIDPSAKWFIAEGA
                     DSPRLSRSVPMKKALDDAMIALYKNGERIMPGNGYPMRLLLPGYEGNMNVKFLRRIKL
                     VDQPAMSMYEARTYSQILPNGKAYRFYFLQEVKSFITSPSHGMMLKGPGLQEISGIAY
                     SGTGRVEKVMVSADGGQSWAQAALQEPVLSKAFTRFRMPWRWDGGPATLLSRAWDEAG
                     NVQPTRAEFVAARGELTKPAPVLTFPNQHYNSITSWGVASNGEVKHVYV"
     gene            complement(5285..5797)
                     /locus_tag="VGA15_26670"
     CDS             complement(5285..5797)
                     /locus_tag="VGA15_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX9591314.1"
                     /translation="MQMNDSQRIPAPKEKVWAALNDPEILKKCIPGCQSLEMSSPTEM
                     TATVVFRVGPVKATFGGKVTLSDLDPPNSYRISGEGSGGVAGFAKGGATVRLESESPD
                     VTILHYEVDAQIGGKLAQLGQRLIDSTAKKLAGEFFAAFGAVVGGEAEAPTEADAAPK
                     SWLGKLTGAT"
     gene            complement(5810..6646)
                     /locus_tag="VGA15_26675"
     CDS             complement(5810..6646)
                     /locus_tag="VGA15_26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX9591315.1"
                     /translation="MTAHVEVMELVARLKAAEETFVLATVVRTVSVTAAKAGAKAIIR
                     PDGTIVAGWIGGGCARGAVLKAAREALADGEPRMVSVQPENLLAELGVKPGENRDGVR
                     FAENMCPSKGTMDIFVEPVLPHPSLVILGASPVALSLAAQARQLGYHVTLAAPAADIA
                     AVSEAHVIVDGFQPRYLNDARRFVVISTQGKGDEAALKASVGIDAAYHAFVGSRRKMA
                     ALRAKLIAEGIDASALDRIKAPAGLDLGAITPEEIAMSILAEITIERRRGQRVLPHTA
                     TT"
     gene            complement(6942..8150)
                     /locus_tag="VGA15_26680"
     CDS             complement(6942..8150)
                     /locus_tag="VGA15_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX9591316.1"
                     /translation="MSCCGTNPGYEELDEVSRLVATKLAAFLKTLRDNAFAVGLHEGR
                     DAAALMAAGYAEKPALLRSAFKHLFSARKSDWEKFDGLFDAFWLGKRVKSRSVTMGSA
                     KAANSPSLKSLQDSKSDRTGREAASDQVPSADEAPEGRTGEGRMEGASRAANLAEIDF
                     RNMADPDQIEEAHTVAARLAKTMRTRLTRRDLARRRGYRLDLRRTIHSNISHGGVPIS
                     LVKRQRKEKPLRLVMLLDASGSMSMYTGVFLRFIHGVLDEFREAEAFLFHTRLAYVSD
                     AMKEKDAGRALDRLSIMAQGAGGGTKIGESLQTFNRWHAARVLHSRSCVMIVSDGYET
                     GNAALLGREMAALAKRCRRIVWLNPMMAWDGYAPEAAGIKAALPHVDLYAPANTLKSL
                     TALEPYLAKL"
     gene            complement(8147..>8317)
                     /locus_tag="VGA15_26685"
     CDS             complement(8147..>8317)
                     /locus_tag="VGA15_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX9591317.1"
                     /translation="VAETLDWAAALVGLEVRDLHDAPEVVHETLMCLLKTHEDKSRVT
                     REVTERLLGKVA"
BASE COUNT         1474 a         2673 c         2542 g         1628 t
ORIGIN      
        1 gaagcgctgc tcgtcgcagg cgcgcatcgc cgcctcgtcg ccctcgcccg cacgcgcaat
       61 cgtctcgggc ggcagatgca aaacctcctc cagttgatag ccgtgcgctt gcccgacgcg
      121 gatcgcttcg ctcccgagcc gggtcgagaa gcggcggatc ggctcgcttt gcaacatctg
      181 tccgccgggc aggccggtgc aggcggagag tccgttgccc atggcgttgg cgaccagttt
      241 cgaccagcgc tcgccccaca gattgccggt taccttggcg ctgtcggcgt agccgaccag
      301 acggcagatt tcttccgccc gtcgcgtgat ccggccgtgc acttcgccgg cgcgaaatac
      361 ggtgtgcgcc gtcccgccct tgccagcgcc ccggtgaatg tggccgggct caggcaggtt
      421 gaccgtgatg ctgctcgcaa tgcaccccag cgtcttgccc cagcctacga tgccggcgat
      481 cgtctcttca ttcatgcagt tctgcagcga taccacgtag ccgtccggcg ccagatactg
      541 ctggatcagc gtggtggccc aggccgtgtc gtaggacttc atgcacacga aggcgatgtc
      601 gaccggcgtc tccttggcca gctgctgcgc gtccgtgacg tgcagcgcgc gcaccggaac
      661 cgagaattcc gcgacgtccc tggcgtgggt gacgcgcagg ccatgcttcc tcatgtgctc
      721 gatgtgctcg ggccagggat cgatgaacgt cacgtcctcg cccgcctgca ccatatgggc
      781 gccggcatac ccgccgacgg cccccgctcc gacaatagct atcttccgac ccatgctttt
      841 ctcccggcat tcccagcagc cgaggctagc gtgccggccg ggctcatcgc aacggttgaa
      901 acgcatcgcg ccaggcgcca tgcgttattc gcttacttgt cctcgttcgt ccatctggcc
      961 atttcgtttg actggccaga ctagacgaca ttttcttagc ccgtagcaac cattaggagg
     1021 cgaccatggt aattacggcg tttctccgat gttgctgatg accacggttg tcttgtcgaa
     1081 gtgcgccgtg atttcaagcg tgccgccgag cgcttcgatg taacggcgca aactactcac
     1141 gtgcatgtca ccgcgctttt caagcttggc cacggcgggc tggttgactg tgagtgcatc
     1201 cgcgacttcc gcttgtgaga gagaacgcgc cttgcgcaac tcctcaagcg tcatcgcctc
     1261 gcgcagcgcg gcggcctttg ccgccacacg tgcccggcgc gcgggtgtga agtccttggt
     1321 cagttcgcta aacggcctgc gtccagtcat ggtatcaatc cttccttcct gatctcggcg
     1381 atgtaaatgt cataaagttg gtcggcgatc gggaccatgc gctcgtaaaa gcgatcatcg
     1441 cccgtcttgt cgccgccaat aagcagaatc gccgaccgcc tgggatcgaa agcgtaaaat
     1501 actcgcagcg gtcgtccacc gctctgcaca cgcaactcac gcatatgagc gtgcttcgat
     1561 ccgttaatcc ctgacgaata gggaaaaggc agttgcggcc cttgttcgat cagcaacaac
     1621 ccagcggcag ttatgtcatc ctgctgcgct tcgctgagac catggtacca ttccgcgaac
     1681 tcgtcagtgt gctcgacgtt ccaagccatc gaaggcagta tattccatta atggaatata
     1741 ttcaagaggc caaatttatg ggtgggacat ccagtgtgcg cggcgcgctc gccttggcca
     1801 acctcggcga tcatcgtggt tcgccggaga attcctgccg cagtgctgac aggcggtcga
     1861 gcgcggcttg gggcagcggg cctttttgga ccgcggcgag ggcgtcctcg aactgttgcg
     1921 gcgtcgccat gccgaccagg atcgtgccca tcgccggctg agacagcgca aaccgcgtgg
     1981 cggcttcggt caggctggcg gcgaaaccct cccctaccag cgggatcaga cggcgtgcgc
     2041 gatcgacatc ggcatcgtag ctcatcgccg aaccaatcgg ctcgggcgcc ggaccagcaa
     2101 tcggatgacg ctcggccgag cccgacaggg cgccgccggc cagcacgcgg atacccacga
     2161 cgccaacgcc ggctgcccgg gtagaatcga acaatcgtcc ataatcctgc gccggatagt
     2221 tcgtcggcaa tgcctcggcg gcagatggat tgagcatgtt gtagacgatt tgagcgctgt
     2281 cgaaggcacg tgcattaatc acttgttgca gtgccgccgt gtcccctacg gccgtcatcc
     2341 cgaggaagcg aatcttcccc tgcgcacgca ggcgttcgaa cgccggtact acgtcgtcga
     2401 gcacctgtcg gacgctcagt gccgatccgc ctcctgtctc ggtaatcgga ttgtgcaggt
     2461 gaaagatatc gacccggtca agacccagtc gcgccagact gccttcgagc gacatcgtta
     2521 cggcgtcggc aacgcggccg aactcgccgg gcggcagccg gaccttggtg ccgacgacca
     2581 cgcccgctgg tttcagcttt tgcaaaacgc ggccaaggtt gttttccgat tcaccattgc
     2641 cgtactgcac cgcggtgtcg aaatagttca cgccagcggc gaccgcccgc gcgatggtac
     2701 gctcctggtc ggcaggatcg ccgcgtacca tgaatccgcc cactgcgccg cagccaaaac
     2761 ccagcaccga gagctgcatt ccggtgcgcc caaagacccg caattgcatc gatgttcctc
     2821 cgactgcata cacacactac tcaagagacg cgccggactt gcggcacgct gatgcggcgc
     2881 tgcttcgatc gcgcacacca tatgtctaga gccttttccg tgtcgataga atcgaaacgg
     2941 ggctctagat tcttgatttg acgcgttttc ttgacgcgaa ccggtttcca cttcgcttga
     3001 aaacgctcta ggcgcgcacc taaatgcgat ccggaaaccg gatgatgaag ttttcgcgat
     3061 tgggcatttt caccttcggc aaggtttgcg cattcatcat atcgttggcg ccgatcaatt
     3121 tgttctccgc gagaatgtag gcggtgagtg cgtagacttc atcatcggtg agcgaacgcg
     3181 gcgtttgaaa cggcatggcg cggcgaatga aatcgaagat cgtcgttgaa tacggccaga
     3241 aattcttgat ggttttctgc gccgcggaga taccggcgat ctcgcgatca ctcaccaagg
     3301 cggcggcgga gccgcctttt ccgttgtcgc catgacacag tgcgcactta tcggcgtaga
     3361 ccacagcgcc ctgcgcgggc gtgccgctgc ctttcggcag tcccgcgcca tcgggcagga
     3421 tgctgatgtc ccaagcagcg atggccgttt catcgatcgg cttaccgaga ttgggcgtct
     3481 cggccaatga ggcgtcggtc atcatcgctg ataacaatgc ggcggaaaag atcgcgcacc
     3541 tagacgtaaa catgcttgac ctccccattg cttgcgacgc cccaactggt gatcgagttg
     3601 tagtgctgat tgggaaaggt caggactggc gccggtttcg tcagttcgcc gcgtgcggcc
     3661 acgaattcgg cgcgggtcgg ctgtacattg ccggcttcat cccacgcccg gctcagcagc
     3721 gtggccgggc cgccgtccca gcgccatggc atgcggaaac gggtgaaggc cttgctcagt
     3781 accggttctt gaagtgccgc ttgcgcccag ctttggccac cgtcggcgga gaccatcact
     3841 ttctcgaccc ggccggtgcc ggaataagcg atgcccgaaa tctcttggag accaggaccc
     3901 ttcaacatca tgccgtgcga agggctggtg atgaaggatt tgacttcctg caggaaataa
     3961 aaccggtacg ctttgccgtt cggcaggatc tgcgagtagg tccgcgcctc atacatgctc
     4021 atggcgggct ggtccacgag cttgatgcgg cgcaggaact tgacgttcat gttaccctcg
     4081 tagccgggca gcagcaggcg catcggatag ccgtttccag gcatgatgcg ctcgccgttc
     4141 ttgtagagcg cgatcatggc gtcatccaac gccttcttca tcggcacgct gcggctcaaa
     4201 cgtggcgagt ccgcgccctc ggctatgaac catttcgcgc tgggatcaat gccggtttcc
     4261 tcaagcaggg tcgaaagttt gattccggtc cactccgcgc aggaggcgag cccgtgtagt
     4321 gcctgcacgg tttcctgcat cggctcggga gagaacatcg gggcgctgtt gccgccgcat
     4381 tcgacgaacg acacgcgcga gatcatggga tagcgcgaca gtgtttcgag cgtgaactcc
     4441 aatggccgct tcaccagtcc atggatcacg agccgatgct tctccggatc gatgttggga
     4501 acgccagcgt gattgatggc gaaatgcagc ccattcggcg tggtcgtacc ctcgaggaga
     4561 tggtgcggcg tgcgcgcgtg tgaattacgc ggctcgtgat taggattact cagcgtgcgc
     4621 gcaaccttgg tctcgaactt cgacggcacc tggtagagcg gcattgggtc gccgacttcc
     4681 atgctccatg gatcatcggc gagcggttcg gcgcccgcgg ccgcagatga cgggccaatg
     4741 cccgcaccca tcgcgcccgc gaacaccatg ccgcgcccaa gcagcgcgcg acggtcgatc
     4801 agcccgtttc cggcagcggc ttccaaagtc tgaccacgcg cattgcgatg cctggcattg
     4861 gccatcctag cctccctcga gtacggacgt tataggtcgt cctttgttct cacgcgctac
     4921 tgtagctgtt tgcgatatgg ttgtctcata cacctcatac acctaataca tcgggcaaaa
     4981 cagcaagtag cccgcatgag cacagcgata tgcgggattc tgaaatatga aaatcccgga
     5041 tatcgctccg ctcatccggg ctacgatgcc ataacactca cggccggtcg tgtcccggac
     5101 gcggcgcagc gttcttcacg ctgctccgca gagccgggac ccacaagctt atgggccccg
     5161 gatcagcagc gcaccacgcc gcaaggcata gcgcgtcgaa gacgcgcgtg aacgcgctga
     5221 tggcgctgcg cagcatccgg ggcacgcagg acttagacat ttgctttgcc tactctacgg
     5281 ttcgctaagt ggcgccggtc agcttgccga gccagctttt cggggcggca tcggcttccg
     5341 tgggcgcctc ggcctctccg ccaacaaccg cgccgaacgc ggcaaagaac tcgccggcga
     5401 gtttctttgc tgtcgaatcg atcaagcgct ggccgagctg cgccagcttg ccgccgatct
     5461 gcgcgtcgac ctcgtagtgc aggatcgtga catcagggct ttcggattcc aggcgcacgg
     5521 tggcgccgcc cttggcaaag cccgcgacgc cgccggagcc ttcgccggaa atacgatagc
     5581 tgttcggtgg atcgagatcg gacagcgtca ccttgccgcc gaacgtcgct ttgaccgggc
     5641 cgacccgaaa caccacggtg gcggtcattt cggtcggcga ggacatttcg aggctttggc
     5701 agcccggaat gcatttcttc aggatttcgg gatcgttgag cgccgcccag actttttcct
     5761 tcggcgcggg gatgcgctgg ctgtcattca tttgcatgat caaatttcct taggtggtcg
     5821 cagtgtgggg aaggacgcgc tgaccgcgcc ggcgctcgat ggtgatctcg gccagaatcg
     5881 acatcgcgat ctcttccggc gtgatggcgc cgaggtcgag gccggccggg gccttgatgc
     5941 ggtcgagcgc gcttgcgtcg atgccttcgg cgatcagttt ggcgcgaagg gccgccatct
     6001 tgcggcggct gccgacgaag gcgtgatagg cagcgtcgat cccgaccgaa gccttcaagg
     6061 cggcctcatc gcccttgccc tgcgtcgaga tcacgacaaa gcggcgggcg tcgttgagat
     6121 agcgcggctg aaagccatcg acgatgacat gggcctctga cacggcagca atatcggccg
     6181 caggtgctgc gagcgtgacg tggtagccga gctgccgcgc ttgggctgcc agcgacaacg
     6241 ccaccgggct cgcgccgaga atgaccagcg agggatgcgg cagcaccggt tcgacaaaga
     6301 tgtccatggt gcccttgctc gggcacatgt tctctgcgaa gcgcacgccg tcgcggttct
     6361 cgccgggctt gacgccgagc tcggccaaga gattctccgg ctgcaccgag accatgcgcg
     6421 gctcgccgtc ggccagcgcc tcgcgcgcgg ccttgagcac cgcgccgcgg gcacagccgc
     6481 cgccgatcca gcccgccacg atggtgccgt ccgggcggat gatcgccttg gcgccggcct
     6541 tggcggcggt caccgacacc gtgcggacta cggtggccag aacaaaagtt tcctcggcgg
     6601 ccttgagccg ggccaccagc tccatcactt cgacatgcgc ggtcatcggc gtctatcctt
     6661 cggtcacctc tccccaccgg ggagaggtcg atttgccaac gggtcggcgc gaagcgccgc
     6721 ccgatgataa actccgcaaa tcgggtgagg gggcgccgct ctaacgagag agcgtaaccc
     6781 ctcacccggc gctacgcgcc gacctctccc atcgaagtcg gatatatccg acttcgacaa
     6841 tctaaatgcc caactcgggt aaacccgagt tgggtgggag aggtaaagtc accctcgttt
     6901 tcgttgcgtc gcagctcaat ccagtctcat ccgatcaagc ctcagagttt cgccagatag
     6961 ggttcaagcg cggtcaggct tttcagcgta ttggcgggcg cgtagagatc gacatgcggc
     7021 aatgcggcct tgatgccggc ggcctcgggc gcatagccgt cccacgccat catcgggttg
     7081 agccagacga tgcggcggca gcgttttgcc agcgccgcca tctcgcggcc gagcagcgcc
     7141 gcgttgccgg tctcatagcc gtcggacacg atcatcacgc aggagcgcga atgcagcacc
     7201 cgcgcggcgt gccagcgatt gaaagtctgc aggctctcgc cgattttggt gccgccgccg
     7261 gcgccctgcg ccatgatcga cagccggtcg agcgcgcggc cggcatcctt ttccttcatc
     7321 gcgtcggaga cataagcaag ccgcgtatgg aacaggaaag cctcggcttc gcgaaattca
     7381 tcgagcacgc cgtggatgaa gcgcaggaac acaccggtat acatgctcat cgagccagac
     7441 gcgtcgagca gcatgacgag acgcagcggc ttttccttgc gctgccgttt gacgaggctg
     7501 atcggcacgc cgccatggct gatattgctg tggatcgtcc tgcgcaaatc cagccggtag
     7561 ccgcgccgcc gcgccagatc gcggcgggtc agccgcgtgc gcatggtttt tgccagccgc
     7621 gctgccaccg tatgggcttc ctcgatctgg tcggggtccg ccatgttgcg gaaatcgatc
     7681 tcggcaaggt tggcggcgcg cgaggcgcct tccatgcggc cctcgccggt gcgcccctcc
     7741 ggcgcctcgt cggcggaggg aacctggtcg gatgcggctt cgcggccggt ccgatcggac
     7801 ttgctgtcct gcaggctctt cagcgacggg ctgttggccg ctttcgccga gcccatggtc
     7861 accgatcgcg atttcacccg tttgccgagc cagaacgcgt cgaacagccc atcgaatttt
     7921 tcccaatcgg atttccgcgc cgagaacaga tgcttgaacg ccgagcgcag caaagccggc
     7981 ttttcggcat aacccgccgc catcagggcc gccgcatcgc ggccttcatg caggccgacg
     8041 gcaaaggcgt tgtcgcgcag cgtcttcaga aacgccgcga gtttcgtcgc gaccaggcgg
     8101 gagacctcgt cgagttcttc gtatcccggg ttggtgccgc aacagctcat gcgaccttcc
     8161 ccagcaggcg ttcggtcact tcgcgcgtga cccgcgactt gtcctcatgg gttttcagca
     8221 ggcacatcag ggtctcgtgg accacttccg gcgcgtcgtg caggtcgcgc acctcgagcc
     8281 cgaccagcgc cgccgcccag tccagcgtct ccgcgac
//
LOCUS       DASWGM010000991         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2076585,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000991 DASWGM010000000
VERSION     DASWGM010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076585"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="VGA15_26690"
     CDS             complement(<1..599)
                     /locus_tag="VGA15_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX9591318.1"
                     /translation="MNEQFGARLTADGASFRLWAPAAKRVDLLLEKALPLRRGENGWF
                     SADVAGVKAGARYKFRIDGEIDVPDPASAFQPDDVSGQSEVIDHTSYRWRASDWRGRR
                     WQETSVLEAHVGTYTPEGTYRAMIGKLDHLVATGITALELMPLADFAGSRNWGYDGVL
                     WYAPDSVYGRPDDLKALIDEAHLRGLMVFLDVVYNHFGP"
     gene            complement(596..>1540)
                     /locus_tag="VGA15_26695"
     CDS             complement(596..>1540)
                     /locus_tag="VGA15_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme GlgX"
                     /protein_id="HEX9591319.1"
                     /translation="LVAEPWDLGMGGYQVGAFPSQWSEWNDRYRSAMRRYWSGEGSLI
                     GEVSSRMTGSSDLFNHDSRAPRAGINHITVHDGFTLADLFSYSEKHNEANGEENRDGS
                     SDNLSNNCGHEGPTDDATIVALRRQLRKNQLACLLLAQGLPLILAGDEVGNTQNGNNN
                     AYCQDNEIGWVGWEGLNRDGDDLTDFIGHMTGLRKRFPQIRSRRWLDGRRADGSYGVL
                     WLTPAAEEMKEQDWNFPEGRFLAYVLGPIEHGQTPIFIVLNAAPEAIGFKLPKLPEYK
                     SWQRLLNTVDSKQTVADFASGSDAEAPPRSVLAFAGSA"
BASE COUNT          272 a          488 c          462 g          318 t
ORIGIN      
        1 tcgggaccaa aatggttata gaccacgtcg agaaatacca tcagcccgcg cagatgcgct
       61 tcgtcgatca gggctttgag atcgtcgggc cgtccataga cgctgtcggg cgcgtaccac
      121 agcacgccgt catagcccca attgcgagaa ccggcgaaat ccgccagcgg catcaattcc
      181 agcgcggtga taccggtcgc caccagatga tcgagcttgc cgatcatggc gcggtaagtg
      241 ccttccggtg tgtaggtgcc gacatgggct tcgagcacgc tcgtttcctg ccaccggcgc
      301 ccgcgccagt ccgaggcgcg ccatcggtag ctggtatgat cgatcacttc gctttggccc
      361 gacacgtcgt caggctgaaa cgcggaagcc ggatcgggca cgtcgatctc gccgtcaatc
      421 cggaatttgt aacgcgcgcc ggcttttacg cccgcgacat cagccgaaaa ccagccgttt
      481 tcgccgcgtc gaagcggcag agctttttcc agcagcagat cgacgcgttt tgccgccggc
      541 gcccatagac gaaatgacgc gccgtccgct gtcagccttg cgccgaattg ctcattcatg
      601 ccgaacccgc aaaagcaagc accgagcgag gcggcgcttc tgcgtcgctg cccgaggcga
      661 aatccgcgac cgtctgtttg ctgtccaccg tgttcagcag tcgttgccag ctcttgtatt
      721 ccggcaactt cggcagtttg aacccgatcg cttcgggggc ggcgttcagc acaatgaaaa
      781 tcggcgtctg gccatgctcg atcggcccca gcacataagc gagaaaccgc ccctcaggga
      841 aattccagtc ctgctccttc atctcttcag ccgccggcgt cagccatagc acgccgtagg
      901 atccgtctgc gcgccgacca tcgagccagc ggcgtgagcg gatttgtgga aagcgttttc
      961 gcaagcctgt catgtgaccg atgaaatcgg tgaggtcgtc gccgtcgcgg ttgagcccct
     1021 cccagccgac ccagccgatc tcgttatcct ggcaataggc gttgttgttg ccgttctggg
     1081 tgttgccgac ttcgtcgccg gccagaatca gcggcaggcc ctgcgccagc agcagacaag
     1141 cgagctggtt ttttcggagc tggcggcgca gtgcgacaat tgtcgcgtcg tcggttgggc
     1201 cttcgtggcc gcaattgttg ctgaggttgt cgctggagcc gtcgcgattt tcctcgccat
     1261 tggcctcgtt atgtttttcg ctgtagctga acaaatccgc caacgtgaag ccgtcgtgca
     1321 ccgtgatgtg attgatgccg gcgcgtggtg cgcggctgtc gtggttgaac aggtcggatg
     1381 agccggtcat gcggctggac acttcgccga tcaggctgcc ttcgccgctc cagtagcgcc
     1441 gcatcgcgct gcgataacgg tcgttccatt ccgaccattg cgacggaaac gcgccgacct
     1501 gatagccgcc cattccgagg tcccacggtt cggcgaccag
//
LOCUS       DASWGM010000992         7421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1924387,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000992 DASWGM010000000
VERSION     DASWGM010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924387"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VGA15_26700"
     CDS             complement(<1..855)
                     /locus_tag="VGA15_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX9591320.1"
                     /translation="MAGADSVEKLEDVIVRSAEQVAGQIRAAKDAISTVIFGQDRVIE
                     NTLVTILSGGHALLIGVPGLAKTKLVETLGITLGLDAKRIQFTPDLMPSDILGAEVLD
                     ESTSGKRSFRFISGPVFAQLLMADEINRASPRTQSALLQAMQEQHITVAGARHDLPKP
                     FHVLATQNPLEQEGTYPLPEAQLDRFLMEIDVDYPDRDAERRILFETTGAEETLAKAA
                     MNAEILISAQRLVRRLPVGDSVVEAILSLVRAARPGPEGGEAGKLIAWGPGPRASQSL
                     MLAVRARAL"
     gene            1111..1719
                     /locus_tag="VGA15_26705"
     CDS             1111..1719
                     /locus_tag="VGA15_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX9591321.1"
                     /translation="MAKQGQTTHQGLEGLTAAVRDVANASPAKGLPPVHLWNPPFCGD
                     LDMRIASNGTWFYLGTPIGRPALVRLFSTILKREDGKHFLVTPVEKVGIRVDDAPFLA
                     VEMLKETDRRGRVLRFRTNVDDWVACDSAHRLRFEAAADGGLTPYLHVRADLWAKVTR
                     VLYYDLVDMGEERVVDGRPMFGIDSAGEFFSMADAEQVRGAL"
     gene            1716..2387
                     /locus_tag="VGA15_26710"
     CDS             1716..2387
                     /locus_tag="VGA15_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX9591322.1"
                     /translation="MNEPVLRMDPAAIGSAEFFDRARARLRFDIPAGLTDASVVPPSG
                     DQGTDRMLQIVAREQPIRPAAVLIPVVAHTQPTVLLTQRAAHLNDHAGQISFPGGKID
                     ATDASPLDAALREAEEEIGLAREFIDPIGYLDLYATGFGFRILPTVARVKPGFRLRIN
                     EAEVDDAFEVPLAFLMNSANHQVHSKEFRGMERSYYAMPFEERYIWGATAGILRVLYE
                     RICLP"
     gene            2384..2647
                     /locus_tag="VGA15_26715"
     CDS             2384..2647
                     /locus_tag="VGA15_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HEX9591323.1"
                     /translation="MIRPAFTEIGIFLIPFAVYALFLIATRSGLLVQSSWPIHIIGRL
                     VLGALVLVVISFILLAHFSGAPPNSTYIPAHIENGKFVPGVEK"
     gene            2644..3900
                     /locus_tag="VGA15_26720"
     CDS             2644..3900
                     /locus_tag="VGA15_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX9591324.1"
                     /translation="MSNARVLDDAPWLKSGPTARVLGLLNGNGEEARVVGGAVRNALL
                     QIPAGDIDIATTALPDEVIRRARAAGIKSVPTGIEHGTVTLVVDAQPFEVTTLREDTE
                     TFGRKAKVAFGRDWVRDAERRDFTINGLSVDAAGVVHDHVGGLDDILAKRVRFIGDPD
                     QRIAEDYLRILRFFRMHAAYGAGEPDRNGYLACIRARAGLATLSAERVRMEMLKLVIA
                     DGAAGAVAAMADGGLLLPIFGGIGYTGPFAAMISAERLLGLKPDAVRRLGALAVAVTE
                     DARRVATRLRLSNAETKALDSLGHRWWRLGGMDEAIARRRLYRLGKDRYRDRLMLAWA
                     RAGAGANSAHWRELATLPERWSAPKFPLKAADFVARGITEGPALGRVLALAEDAWLAA
                     DFPQDGPALKAIADQTVIRFTRDHRP"
     gene            3897..4664
                     /locus_tag="VGA15_26725"
     CDS             3897..4664
                     /locus_tag="VGA15_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX9591325.1"
                     /translation="MNILSGFSDISIAQLLLVTSVALFASVVGGLAGYGTGALMPLVL
                     VPLVGAEPVVPIIAISAIFTNSSRFAAYLRHADLRRAGIVVAAAALTTALGAYGYTRL
                     SNAGAALVIGAMLISSVPLRRSLKRRDVKIGDTGLAAGAVGYGVLVGGTSGSGVILLS
                     LLMAAGLEGAAVIATDAVISVTTSMIKISVFGLSGVVTAQVLAFAVLIGAIALPGAFL
                     AKVFVERMPVHIHTAILDVAVMVGGVVMISAAVRHLW"
     gene            complement(4797..5003)
                     /locus_tag="VGA15_26730"
     CDS             complement(4797..5003)
                     /locus_tag="VGA15_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HEX9591326.1"
                     /translation="MGLKPGDVVVLKSGGHPMTVAEVDDDDIACLWMGTEGELFRETL
                     SLAVLEAAEIEDDDEEEDDEEEEE"
     gene            complement(5212..6099)
                     /gene="hemF"
                     /locus_tag="VGA15_26735"
     CDS             complement(5212..6099)
                     /gene="hemF"
                     /locus_tag="VGA15_26735"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX9591327.1"
                     /translation="MDMSVIEDRKARARSWFESLRDDICLAFEQLEDDALASLYPGGA
                     GRFVRTPWDRTDHTGRPGGGGLMSVMRGRLFEKVGVHCSTVHGEFSPEFRSQVQGARE
                     DPNFWASGISVIAHLRNPHVPAVHMNTRFVVTTKAWFGGGSDLTPVLDRRRTQQDADT
                     IAFHAAMQAACGGHNGIADYDKYKKWCDDYFYLPHRKEARGIGGIFYDHHDSGDWNAD
                     LAFTQEVGRAFLKIYPELVRRNFDAPWTAQDREEQLIRRGRYVEFNLLYDRGTIFGLK
                     TGGNVDSILSSMPPEVKWP"
     gene            complement(6160..6525)
                     /locus_tag="VGA15_26740"
     CDS             complement(6160..6525)
                     /locus_tag="VGA15_26740"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX9591328.1"
                     /translation="MWELTKSFRFEAAHALGGTTLGAASEEIHGHSFRAEVTVRGTPD
                     PATGMVLDLGLLERSMTEVRKMLDHKLLNKVDALGPPTLENLSRFIWDRVQRAGPITR
                     VSVYRDSCNESCAYYGPQG"
     gene            complement(6541..7173)
                     /gene="queE"
                     /locus_tag="VGA15_26745"
     CDS             complement(6541..7173)
                     /gene="queE"
                     /locus_tag="VGA15_26745"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX9591329.1"
                     /translation="MSYAIKEIFLTLQGEGAHAGRAAVFCRFSGCNLWSGREQDRAAA
                     TCRFCDTDFVGTDGTLGGRYATPGELADTVAAQWTGIGANHYVVLTGGEPLLQVDKAL
                     VDALHARGFEIGIETNGTIEPPEGMDWICVSPKAGADLVIRQGHELKLVYPQADAAPE
                     AFVDLAFERFSLQPMDGPDVIENTARTIDYCLRHPQWRLSLQTHKTLGIR"
     gene            complement(7187..>7421)
                     /locus_tag="VGA15_26750"
     CDS             complement(7187..>7421)
                     /locus_tag="VGA15_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX9591330.1"
                     /translation="RLILFTTKGTNSYLDHRYGAADILLFGRESAGVPDEVVAAADAR
                     LVIPIKPGLRSLNVAMAAAMALGEALRQTSSAK"
BASE COUNT         1271 a         2354 c         2334 g         1462 t
ORIGIN      
        1 caaggcgcgc gcgcgaacag ctaacatcag cgactggctg gcgcggggac ccggtcccca
       61 ggcgatcagc ttgccggcct cgccgccctc cggaccggga cgcgcggcgc gtaccagtga
      121 gaggatcgct tcgacgacgg agtcgccgac cggcagccgc cgcaccagcc gttgcgccga
      181 tatcaggatc tcggcgttca tggccgcctt ggccaatgtc tcttcggcac cggtggtttc
      241 gaacagaatg cgccgctcgg cgtcgcgatc cggataatcg acgtcgatct ccatcaggaa
      301 gcgatcgagc tgcgcctccg gcagcggata ggtgccttcc tgctccagcg ggttttgggt
      361 cgcgagcaca tgaaacggtt ttggcagatc gtggcgtgcg ccggccaccg tgatgtgctg
      421 ctcctgcatc gcctgcagca gcgcggactg ggtgcgcggg ctggccctgt tgatttcgtc
      481 ggccatcaaa agctgtgcga acaccggtcc ggaaatgaaa cggaacgacc gcttgccgga
      541 agtgctctcg tccagcacct ccgcacccag aatatccgag ggcatcaggt ccggcgtgaa
      601 ctggatgcgc ttggcatcga gcccgagggt aatgcctaag gtttcgacca gcttggtctt
      661 ggcgaggccg ggcacgccga tcagaagcgc gtgaccgccc gacagaatcg ttaccagggt
      721 attttcaatc acccggtcct gaccgaagat aactgtggaa atcgcatctt tcgcggcgcg
      781 gatctggccg gcgacctgct cggccgaacg gacgatcacg tcctcgagtt tttcgacact
      841 gtctgcgcca gccattggtc actccttgcc ttaaacgtcg ccggatgcga cgcgatgtca
      901 tgaacccctt gcacaaccta tgcgaaggcc atgtattcgt tgaattaaac tatccccaca
      961 ttatcggcat ttcggggtat gaactgcatc acgatgtggc gactttacct taggatcggt
     1021 tccgggaact cgccgagaca ctcaccggat gacttgcaga agtcttgcac caatcgtgcc
     1081 aaaacgaggc catcaaactc agggcaaacc atggcgaagc aagggcagac cacccatcaa
     1141 ggcctcgaag gactgactgc tgccgtcaga gacgttgcaa acgccagccc cgcaaagggg
     1201 ctgccccccg tgcatttgtg gaacccgccg ttctgcggcg atctcgacat gcgaatcgcc
     1261 agcaatggta cgtggtttta tttggggacg ccaatcgggc ggccggcttt ggtccggctg
     1321 ttctcgacaa tcctcaagcg cgaggacggc aagcactttc tcgtgacacc ggtggagaag
     1381 gtcggcatcc gcgtcgacga tgcgccgttt ctcgccgtcg agatgctcaa ggaaaccgat
     1441 cgtcggggcc gagtgctgcg cttccgcacc aatgtcgacg actgggtggc ttgcgattcc
     1501 gcccaccggc tgcgcttcga ggccgcggcc gacggcggcc tcacgcccta tcttcatgtc
     1561 cgcgccgacc tgtgggccaa ggtgacgcgg gtgctttact acgatctggt tgacatgggc
     1621 gaggagcggg tggtcgatgg tcgtccaatg ttcgggatcg attcagccgg cgaattcttt
     1681 tcgatggcgg atgcggagca ggtgaggggc gcgctttgaa cgagccggtg ctgaggatgg
     1741 accccgctgc gatcggttcg gcggagtttt tcgatcgggc ccgcgcgcgg ctgcgcttcg
     1801 acatccccgc gggattgacc gacgccagcg tggtgccgcc gagcggcgat cagggcaccg
     1861 atcgcatgct gcagatcgtc gcccgcgaac agccgatccg tcccgcggcg gtgttgatcc
     1921 cggtggtggc gcatacgcaa ccgaccgtgc tgttgacgca gcgcgcggcg catctcaacg
     1981 atcacgccgg ccagatctcg ttccccggcg gcaagatcga cgcgaccgac gcttcgccgc
     2041 tcgacgcggc gttacgcgag gccgaggagg aaatcggcct tgcgcgggaa ttcatcgatc
     2101 cgatcggcta tctcgatctg tacgcaaccg ggttcggatt tcgcatcctg ccgacggtcg
     2161 cgcgggtgaa gccgggcttc aggctgcgca tcaacgaagc cgaagtggac gatgcgttcg
     2221 aggtaccgct ggcgttcctg atgaattctg cgaatcatca ggtgcacagc aaggaattcc
     2281 gcggcatgga gcgctcctac tacgccatgc cgttcgagga gcgttatatc tggggcgcaa
     2341 ccgcggggat tctgcgtgtg ctgtatgagc ggatctgcct gccatgatcc gtccggcctt
     2401 caccgaaatc ggaattttcc tgatcccgtt cgcggtgtat gcgctgtttt tgatcgcgac
     2461 gcgctccgga cttctggtgc agtcgtcatg gccgatacat atcattggca gactggtgct
     2521 cggtgcgctg gtcctggtgg tgatcagttt tatcttgctg gcgcatttct ccggcgcgcc
     2581 gccgaattcg acttacattc ccgcgcatat cgagaacggc aagttcgttc ccggagttga
     2641 aaaatgagca atgcgcgcgt gctcgacgac gcgccctggc tcaaatccgg cccaaccgcc
     2701 cgcgtgctcg gattgctgaa cggcaatggc gaggaagcgc gcgtggtcgg cggcgcggtc
     2761 cgcaatgcgc tattgcagat tcctgccggc gatatcgata tcgcgaccac cgccttgccc
     2821 gatgaagtga tccgccgcgc ccgggcggcc ggcatcaaga gcgtcccgac cggcatcgaa
     2881 cacggcaccg tgacgctggt cgtcgatgcg cagccgttcg aggtcaccac cctgcgcgag
     2941 gataccgaga cgttcggccg caaggccaag gtcgcattcg gccgcgactg ggtccgcgac
     3001 gccgagcggc gcgacttcac catcaacggg ctgtcggttg atgccgccgg tgtcgtgcac
     3061 gatcacgtcg gcgggctcga tgatatcctg gccaaacggg tgcgcttcat cggcgacccc
     3121 gatcagcgga tcgccgaaga ctatctgcgc atcctcaggt ttttccgcat gcacgccgcc
     3181 tacggcgcgg gcgagcccga tcgcaatggg tacctcgcct gcattcgcgc gcgcgccggc
     3241 cttgcgaccc tttccgcaga acgcgtgcgg atggaaatgc tgaagctggt gatcgccgat
     3301 ggcgctgccg gcgcggtcgc agcgatggcg gatggcgggt tgctgctgcc gattttcggc
     3361 ggcattggct acaccgggcc gttcgctgcc atgatctcgg cggagcgcct gcttggactg
     3421 aagcccgacg cggttcgtcg cctcggcgcg ctcgcggtcg ccgtcaccga ggacgccagg
     3481 cgggttgcaa cgcgcttgcg gctctccaat gccgaaacaa aggcgctcga ttccctgggg
     3541 catcgctggt ggcggctcgg cggcatggac gaggcgatcg ccaggcgacg gctctatcgg
     3601 ctcggcaagg accgctaccg cgaccggctg atgctggcgt gggcgcgggc gggagcgggc
     3661 gctaattccg cgcattggcg cgaactcgca accttgccgg agcgctggag tgcgccgaaa
     3721 tttccgctga aggcggccga tttcgttgcg cgcgggatca ccgaaggtcc ggcgctcggg
     3781 cgggtgctcg cgctggccga agacgcgtgg ctggcggcgg atttccctca ggatggacct
     3841 gcgctaaaag ccatcgccga ccaaaccgtc atccgtttca cccgcgacca tcggccgtga
     3901 acatcctttc cggtttttcc gatatctcga tagctcaact gctgctggtg acgagcgtcg
     3961 cgctgtttgc ctctgtcgtc ggcgggctgg cgggttacgg caccggtgcg ctgatgccgc
     4021 tggtgctggt gccgctggtc ggcgccgaac cggtggtgcc gatcatcgcg atctccgcga
     4081 tcttcaccaa cagcagccgt ttcgccgcct atctccgtca tgccgatctc cggcgcgcgg
     4141 gaatcgtggt cgcggcggct gcgttgacga ccgcgctcgg cgcttacgga tatacccggc
     4201 tcagcaacgc cggcgcggcg ctggtgatcg gcgccatgct gatatcgagc gtgccgctgc
     4261 gccgttcgct caagcgccgc gatgtcaaaa tcggcgacac cgggctcgcc gccggggcgg
     4321 tgggctacgg cgtcctggtc ggcggcactt cgggctcggg cgtgatcctg ctctcgctgc
     4381 tgatggcggc gggcctcgaa ggcgcggccg tgatcgccac cgacgcggtg atctcggtca
     4441 cgacaagcat gatcaagatt tcggtattcg gactgagcgg cgtcgtcacc gcgcaagtgc
     4501 tcgcctttgc cgttctgatc ggcgcgatcg cgctgcccgg cgcgtttctc gcaaaagtct
     4561 ttgtcgagcg catgccggtc catattcaca ccgcgatcct cgatgtcgct gtcatggtgg
     4621 gcggcgtggt catgatttcc gcagcggtgc ggcatctctg gtagagaaaa tccgggaata
     4681 cgatttcggg gattacgatt tccgggaata cgatgccgcc gggtccaaaa ggggttcggc
     4741 ggcatcgcag gcgaagtcac gccgcccgcg cttttcctgg gctcctcttc cccgtcctat
     4801 tcttcctctt cctcgtcatc ttcctcttcg tcgtcgtcct cgatttcggc cgcctcaagc
     4861 acggctaatg aaagcgtttc acgaaacagt tcgccttctg tgcccatcca caggcacgcg
     4921 atgtcgtcgt cgtcgacttc tgccacggtc atcggatgac cgccggattt cagcacgaca
     4981 acatcgcccg gtttcaaacc catgatttct ttccccaaag ttgattgaca tgattcatgg
     5041 cctgtcgatc tgtcaggaca gaccgatcac ggccacttca ccttgagagg attacccgct
     5101 tctgtgtccg gtctgtgtcg gttccgaaca ttccggcggg cagtggctcc gttgcctacg
     5161 acaatggcaa aatagcaaca ctttggaacg cttgggctga cttgagcgcc gctacggcca
     5221 tttcacttcc ggcggcatcg acgacaggat ggaatctaca ttgccgccgg ttttcagccc
     5281 gaagatggtg ccgcgatcgt aaagcagatt gaattcgacg taacgtccgc gccggatcag
     5341 ctgttcttcg cggtcttgcg cggtccacgg cgcatcgaaa ttgcgcctga cgagttcggg
     5401 atagattttc aggaaggcgc ggccgacctc ctgcgtaaag gcgaggtcgg cattccagtc
     5461 gccgctgtcg tgatgatcat aaaaaatacc gccgatgccg cgggcctcct tgcgatgcgg
     5521 cagatagaaa tagtcatcgc accacttctt gtatttgtcg tagtccgcga tcccgttgtg
     5581 cccgccacag gccgcttgca tcgccgcatg aaacgcaatc gtatcggcgt cttgctgggt
     5641 ccgccgccgg tcgagcaccg gcgtcagatc cgagccgccg ccgaaccatg ccttggtcgt
     5701 caccacgaag cgcgtgttca tgtgcacggc gggaacgtgc gggttgcgca gatgcgcgat
     5761 caccgagatg cccgacgccc agaaattcgg atcctcgcgc gcgccttgaa cttgcgaacg
     5821 gaattcgggg ctgaattcgc cgtgcaccgt ggaacaatgc acgccgacct tttcgaacag
     5881 ccgtccgcgc atcaccgaca tcaggccgcc gccgccgggc ctgccggtat ggtcggtgcg
     5941 gtcccagggc gtgcgcacga agcggcccgc gccgccggga taaagcgatg ccagcgcatc
     6001 gtcctccagc tgttcgaagg ccaggcagat gtcgtcgcgc aagctttcga accacgatcg
     6061 cgcgcgggcc ttgcgatctt cgatcacgga catgtccatg cgatttccgc cttagtttga
     6121 agcgtttttc tacgcgaacc cacttcgctt gaaaaacgcc tagccttgtg ggccataata
     6181 agcgcagctc tcgttgcaac tgtcacggta tacgctcacc cgcgtgatcg ggcccgcgcg
     6241 ctgaacccga tcccagatga agcgcgaaag gttctccagc gtcggcggtc caagcgcgtc
     6301 gaccttgttg agcaatttgt ggtcgagcat cttgcgcacc tcggtcatgc ttcgttcgag
     6361 aaggccgagg tccagtacca tgcctgtcgc gggatcgggc gtgccgcgca ccgttacctc
     6421 ggcacggaac gagtggccat ggatctcctc gctcgccgcg ccaagcgtcg ttccgcccag
     6481 cgcatgggcg gcctcgaacc ggaacgattt cgtcaattcc cacatcctga aaatccagat
     6541 ctacctgata ccgagtgtct tgtgggtctg caggctgagc cgccattgcg gatggcgcag
     6601 acaatagtcg atcgtgcgcg cggtgttttc gatcacgtcc ggtccgtcca tcggctgcag
     6661 cgagaaacgt tcgaaggcga gatcgacgaa agcttccggg gcggcatcgg cctgcggata
     6721 gaccagcttg agttcatggc cttgccggat cacgaggtcg gcgccggcct tcgggctgac
     6781 gcagatccag tccatgccct cgggcggctc gatcgtgcca ttggtctcga tgccgatttc
     6841 gaagccgcgc gcatgcagcg catcgacgag ggccttgtcc acctgcagca atggttcgcc
     6901 gccggtcaat accacgtaat ggttggcgcc gatgccggtc cattgcgcgg cgacggtatc
     6961 cgcaagttcg ccgggcgttg catagcggcc gcccagcgtg ccgtcggttc cgacaaaatc
     7021 ggtgtcgcaa aaccggcatg tcgccgcggc gcggtcctgc tcgcggccgc tccagaggtt
     7081 gcagccggag aaccggcaaa acaccgcggc ccggccggca tgcgcgcctt cgccctgcaa
     7141 ggtcagaaag atttccttta ttgcgtaact cacccgcacg atctcctcat tttgcggaac
     7201 tggtttgccg cagcgcctcg cccaatgcca tcgccgccgc catcgcgaca ttcagcgagc
     7261 gcaagcctgg cttgatcggg atcaccagcc gcgcatcggc ggcggcaacg acctcgtcgg
     7321 ggacccctgc ggactcgcgc ccgaacagca ggatgtcggc cgcgccgtag cgatgatcaa
     7381 gataggagtt ggtacccttg gttgtgaaca ggatcagccg g
//
LOCUS       DASWGM010000993         4234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_968292,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000993 DASWGM010000000
VERSION     DASWGM010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968292"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VGA15_26755"
     CDS             <1..213
                     /locus_tag="VGA15_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX9591331.1"
                     /translation="LQTADPAATVGRLIEEREPVYQHADITIWSRDVPHEKIVDECVE
                     ALHSWLCGDGTAPATQETSDPMGATR"
     gene            210..1364
                     /gene="aroB"
                     /locus_tag="VGA15_26760"
     CDS             210..1364
                     /gene="aroB"
                     /locus_tag="VGA15_26760"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX9591332.1"
                     /translation="MTAPLKRSEPVTVDVALGERAYEIVIGRGVLPSLGARVAALRPG
                     ARTAIVTDRNVARHWLEKTETSLSEAGIASSKIIVDEGEGSKSYAGLEQVSEALIAAR
                     IERNDLVVALGGGVVGDLAGFAAAILRRGVDFVQVPTSLLAQVDSSVGGKTGINSPRG
                     KNLLGAFHQPVLVVADTAVLDTLSPRQFRAGYAEVAKYGVLGDEAFFAWLESNHADIF
                     GGGAAREHAIATSCRAKAAIVARDERETGERALLNLGHTFGHALEAATGFSDRLFHGE
                     GVAVGMVLAAEFSAQLGMISESDAARVKHHLAEVGLPTHLQDIAGFTQEGLGDADALL
                     TLMAQDKKVKRGKLTFILLQAVGRAVIAPDVEPSLVRDFLSARLARKTRA"
     gene            1433..2740
                     /locus_tag="VGA15_26765"
     CDS             1433..2740
                     /locus_tag="VGA15_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEX9591333.1"
                     /translation="MDWLTFSIVIALLLVSAFFSASETALTGASRASMLRLSKQGNRE
                     AGVVSSLFAMRERMIGALLLGNNIANIGASALATGIFTAWFGEVGVLYATGVMTVMVV
                     IFAEVLPKTIAINAPDRVSLLVARPMKVMVYLLGPLLTVIEAIVRVLMRALGIKIGIN
                     QPILSPTERLRGAVDLLHHEGKVEKQDRDMFGGLLDLSELQVSDVMVHRTEMVMINAD
                     LPPEELVREMLATEYTRIPLWREKPENIIGVLHAKDLLRAIRAAEGDTSKIDVSTIAL
                     PPWFVPEMRPVSEQLKAFRRRKTHFALVVDEYGEVEGMVTLEDILEEIVGDISDEHDV
                     VVAGVRAQPDGSVVVDGSVPIRDLNRAMDWHLPDEEATTVAGLVIHEARSIPERGQSF
                     TFHGFRFRVLRRERNRITALRIVAVPREAEVEEKKPKRAGTAF"
     gene            complement(2772..3056)
                     /locus_tag="VGA15_26770"
     CDS             complement(2772..3056)
                     /locus_tag="VGA15_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX9591334.1"
                     /translation="MSAEKTVQHTITNKLREAFAPESLDVTDESHLHEGHAGHRPGGE
                     THFRVYIVSPAFEGKSRIERHRMINATLAAELAASVHALAIKALAPGEAG"
     gene            3165..3830
                     /locus_tag="VGA15_26775"
     CDS             3165..3830
                     /locus_tag="VGA15_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX9591335.1"
                     /translation="MPIDSSKFFDSIRIKPNKLSAKQQAQAREAATMCEWPDCKNKGP
                     HRAPKGRANDKEYWHFCLNHVREYNQSYNFFSGMNADAVARYQKDALTGHRPTWKMGA
                     NTSSKKGKGNPEADLEGASDPFSMFSELNGRGRWRPGPGSGIGDEPKPETRKIFNAER
                     KALQVMGLGPDATLEVVKAKYKALVKQHHPDANGGDRSTEDRLIEIIKAYNYLKTVVR
                     GAT"
BASE COUNT          797 a         1316 c         1332 g          789 t
ORIGIN      
        1 ctgcagaccg ccgatcccgc cgcgaccgtc gggcgtctga tcgaggagcg cgaaccggtc
       61 tatcagcatg ccgacatcac gatctggtcg cgcgacgtgc cgcacgagaa aatcgtcgac
      121 gaatgtgtcg aggcgttgca cagctggctg tgcggcgacg gcacggcccc ggctacgcag
      181 gaaacatccg accccatggg cgccacccga tgaccgcgcc gctgaaacgt tccgaacccg
      241 tcacggtcga tgtcgcgctt ggcgagcgcg cctatgagat cgtgatcggc cgcggcgtac
      301 tgccatcgct gggcgcccgt gtcgccgcgt tgcggcccgg cgcgcggacg gcgatcgtaa
      361 ccgatcgcaa cgtggcaagg cattggcttg agaagaccga gacctcgctg tcggaagccg
      421 gcattgcttc gtcaaaaatc attgtcgacg aaggcgaagg ctcgaaatcc tatgccggtc
      481 tcgaacaggt cagcgaggcg ctgatcgcgg ccaggatcga gcgcaacgat ctggtggtcg
      541 cgctcggcgg cggcgtggtc ggcgatctcg ccggcttcgc ggccgcgatc ctgcgccgcg
      601 gcgtcgattt cgtgcaggtg ccgacctcgc tgctggcgca ggtcgactcg tcggtcggcg
      661 gcaagaccgg aatcaattcg ccgcgcggca aaaatctgct cggcgcgttt caccaaccgg
      721 ttctggtggt cgcggacacc gcggtgctcg atacattgtc gccgcgccag ttccgcgccg
      781 gctatgccga agtcgccaaa tacggcgtgc tcggcgacga agcgttcttt gcctggctgg
      841 agagcaacca cgccgatatt ttcggaggcg gtgcggcgcg cgaacatgcc atcgccactt
      901 cctgccgcgc caaggccgcg atcgtggcgc gcgacgagcg cgagaccggc gagcgcgcgc
      961 tgctcaatct cggtcatacc tttggccacg cgctggaggc cgcgaccggc ttttccgacc
     1021 ggctgttcca tggcgaaggc gttgccgtcg ggatggtgct ggcggcggaa ttttccgcgc
     1081 agctcggcat gatctccgaa tcagatgccg cgcgcgtcaa gcatcacctt gccgaagtgg
     1141 gtctgccgac ccatctgcag gacatcgcgg gatttaccca ggagggactc ggcgatgccg
     1201 acgcgctgct gacgctgatg gcgcaggaca agaaggtcaa gcgcggcaag ctcaccttta
     1261 ttctgttgca ggcggtcggt cgcgccgtga ttgctcccga tgtcgagccg tcgctggttc
     1321 gcgattttct gagcgccagg ctggcgcgca aaacccgagc gtgatttgga agggcggaaa
     1381 ccggctttcc aaccgatcat gctccaacga tagatgaagt gaataccacg ccatggactg
     1441 gcttacattc tccatcgtca ttgccttgct gctggtctca gcgttcttct cggcgagcga
     1501 gaccgcgctg accggcgcgt cgcgcgccag catgctgcgg ctgtcgaagc agggcaaccg
     1561 cgaggccggt gtcgtctcca gcctgtttgc gatgcgcgag cggatgatcg gtgcgctgct
     1621 gctcggcaac aacatcgcca atatcggcgc ttcggcgctg gcgaccggca ttttcaccgc
     1681 ctggttcggc gaagtcggcg tgctctacgc caccggcgtg atgacggtga tggtcgtgat
     1741 cttcgccgag gtgctgccga aaaccatcgc catcaacgcg ccggaccggg tgtcgctcct
     1801 ggtcgcgcgg ccgatgaagg tgatggtgta tctgctggga ccgctactca ccgtgatcga
     1861 ggcgatcgtt cgcgtgttga tgcgggcgct gggcatcaag atcggcatca accagccgat
     1921 cctgtcgccg accgagcgac tgcgcggcgc ggtcgatctg ctccatcacg aaggcaaggt
     1981 cgaaaagcag gaccgcgaca tgttcggcgg gctcttggat ctgagcgagc tgcaggtctc
     2041 cgacgtcatg gttcaccgca ccgaaatggt gatgatcaac gccgacctgc cgcccgaaga
     2101 actggtgcgc gagatgctgg ccaccgaata taccaggatt ccgctatggc gcgaaaagcc
     2161 ggaaaatatc atcggcgtgc tgcatgccaa ggatctgctg cgcgcgatcc gcgccgccga
     2221 aggcgatacc tccaagatcg acgtctcgac gatcgcgctg ccgccatggt tcgtgccgga
     2281 gatgcgtccg gtatccgaac agctcaaggc gttccgccgc cgcaaaaccc acttcgcgct
     2341 ggtggtcgac gaatacggcg aagtcgaagg catggtgacg ctggaggata ttctggaaga
     2401 aatcgtcggc gatatttccg acgagcacga tgtggtggtg gccggcgttc gcgcccagcc
     2461 cgacggttcg gtggtggtcg acggctcggt gccgatccgc gatctcaacc gcgcgatgga
     2521 ctggcacctg ccggacgaag aggcgaccac cgtggcgggc ctggtgattc acgaggcgcg
     2581 ctcgattccg gaacgcggcc agagctttac gttccacgga tttcgtttcc gcgtcctgcg
     2641 ccgcgagcgc aaccgcatca ccgcccttcg gatcgtggca gtgccgcgcg aggccgaggt
     2701 cgaagagaaa aagccgaagc gggcgggaac ggcgttttag gtgcgatttt cgtcaaatgt
     2761 tggcagcgct atcagccagc ttcccccggc gccagcgcct tgatcgccag cgcgtgcacg
     2821 cttgccgcga gttccgctgc cagcgtcgcg ttaatcatgc gatggcgttc gatccggctc
     2881 ttcccttcga aggcaggcga cacaatatag accctgaaat gcgtctcgcc gcccggcctg
     2941 tggccggcgt ggccctcatg cagatgtgat tcgtccgtga cgtcgaggct ttccggcgcg
     3001 aaagcttcac gcaacttgtt tgtgatggta tgctgcacgg ttttttcggc gctcatgtcg
     3061 gcggcaatac gtccatgacc gcgtttcgtc aatggcgcat caagagcgag ggtttcgcaa
     3121 tgtcaagact tgaagctttt cgcattgcgt tgtcatagtc agacatgccg attgattcat
     3181 ccaaattctt cgactccatt cgcatcaagc ccaacaagct gagcgcgaag cagcaggcgc
     3241 aggcgcgcga agccgccacg atgtgcgaat ggcccgactg caagaacaag gggccgcacc
     3301 gcgcccccaa gggccgcgcc aacgacaagg agtactggca cttctgtctc aatcacgttc
     3361 gcgagtacaa ccagtcctat aatttcttct ccggcatgaa tgccgacgcg gttgcgcgtt
     3421 accagaagga tgcgctgacc ggccaccgtc cgacctggaa gatgggcgcc aacaccagct
     3481 ccaagaaggg caagggcaac ccggaagccg atctggaagg cgcgtccgat ccgttcagca
     3541 tgttcagcga actcaatggc cgcggccgct ggcggcccgg tccgggctca ggcattggcg
     3601 atgaacccaa gccggaaacc cgaaaaatat tcaacgccga gcgcaaggcg ctgcaggtga
     3661 tgggcctcgg tccggatgcg acgctcgagg tcgtcaaggc gaaatacaag gcgctggtga
     3721 agcagcacca tcccgatgcc aatggcggcg atcgctcgac cgaagatcgc ctgatcgaaa
     3781 tcatcaaggc gtataattat ctcaagaccg tcgtgcgcgg cgcgacctga gtttttgggg
     3841 tggcgttccg ccgggacgac acgcggagcg tcgtcccgct tgcacggcgg ccttcggcgt
     3901 cgcgccaaac ccacgagacg gcgcatctgc acgcccggcg tcttttccag ttactaagtc
     3961 tgcatcgagg gcgccgcttg aggtcacggt gtattgttgg acgatcagat tttcttatca
     4021 atgaagcctc ctattacgat tagacctgat cgacaacctc ttctaccgtg tagtcaatcc
     4081 gcacccagca ccagcgatct cccccactgc accggcggca gagtgcgttc gatcacgaca
     4141 acaacttcaa tatgaaagga attgtggatc atgaccgcgc cccaacgagc tcagttttcc
     4201 aaccatgcta atctcgtcga tgagatcttc acgg
//
LOCUS       DASWGM010000994         6388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1225142,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000994 DASWGM010000000
VERSION     DASWGM010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225142"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VGA15_26780"
     CDS             complement(<1..615)
                     /locus_tag="VGA15_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9591336.1"
                     /translation="MATTAATPAHSDHVHDDHAHDHPTGWRRFVYSTNHKDIGTMYLV
                     FAIIAGIIGAAMSIMIRIELMYPGVQIFHETHTYNVFVTSHGLIMIFFMVMPAMIGGF
                     GNWFVPLMIGAPDMAFPRMNNISFWLLPASFALLLISTFVEGEPGANGVGAGWTLYAP
                     LSTSGHPGPAVDFAILSIHLAGASSILGAINFITTIFNMRAPGMT"
     gene            complement(710..1549)
                     /gene="coxB"
                     /locus_tag="VGA15_26785"
     CDS             complement(710..1549)
                     /gene="coxB"
                     /locus_tag="VGA15_26785"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX9591337.1"
                     /translation="MKMSKGQTGRRLLGLAVAGVALLTGGAAFAELGQPAPWEYKLQE
                     AATPVMDNITWFHNFLLVLITVITLFVLALLVIVVVKFNAKSNPVPSRTTHNTLIEVA
                     WTLIPVLILVSIAVPSFRLLFQELDIPKADLTIKATGKQWYWSYAYPDNGKFEFDSLL
                     AQDKQPRLLGVDNEMVVPINKVVRIQTTGADVIHSFAIPAFGIKIDSIPGRLNETWFK
                     ATKLGMFYGQCSELCGKDHAFMPIAVRVVNDQEFATWVDGAKKKFATNPANSLAWAGA
                     AAQ"
     gene            2017..2592
                     /locus_tag="VGA15_26790"
     CDS             2017..2592
                     /locus_tag="VGA15_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX9591338.1"
                     /translation="MRPWKHQARGDANRSNSLAAGFGRLLAANLTAASLAVALFFGLT
                     VAASAQGAVRSVHGDWQIRCDTPPGAQAEQCALIQSVVAEDRSNAGLTVIVLKTADQK
                     SKLMRVVAPLGVLLPSGLGLKLDNQDVGRAGFVRCLPNGCVAEVVMDDKLLGQLRTAK
                     TATFIIFETPEEGIGFPLSLNGIGEGYDKLP"
     gene            2820..4244
                     /gene="tldD"
                     /locus_tag="VGA15_26795"
     CDS             2820..4244
                     /gene="tldD"
                     /locus_tag="VGA15_26795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX9591339.1"
                     /translation="MTNLATTSLLDRANLDRDAVRHEVARGLSGADDGELFLEYAQTE
                     ALMFDNGRLKQATYDTSQGFGLRAVKDDAIGYAHSSEVSLPALVRAAEAVQAVRGGYS
                     GNFAAAPAHTNVRLYGDDNPLEAQDFESKVKLLAEIDAYVRGRDPRVRQVSVSVAATW
                     QVVEIVRPDGESYRDIRPLVRVNISVVAGQGDRQESGSKGYGGREGYARFIETKAWRE
                     AADGAIREAMVNLESGPAPAGEMDVVLGAGWPGVMLHEAVGHGLEGDFNRKQTSAFAG
                     LMGQQVAAKGVTVVDDGTMAARRGSLSIDDEGTPTNRTVLIEDGILVGYMQDRQNARL
                     MNMKPTGNGRRQGYAHVPMPRMTNTYMLAGQRDPAEILASVKNGIYAANFGGGQVDIT
                     SGKYVFQCTEAYKIEDGKIGAPLKGAMLIGNGPTDLHRITMIGNDLALDTGIGTCGKN
                     GQGVPVGVGQPTLRMERITVGGTG"
     gene            4244..5014
                     /gene="lepB"
                     /locus_tag="VGA15_26800"
     CDS             4244..5014
                     /gene="lepB"
                     /locus_tag="VGA15_26800"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX9591340.1"
                     /translation="MSIGKESVASRAAGWGAQLLQLAAIVGIVFVAKGALAEPFYVPS
                     GSMEPTLLIGDALLASKYPYGYGTASLPIQINLPETGRVFGEPPKLGDVVVFRWPGDH
                     SQAWVKRVVGLPGDRIQMRQGQLFINDHAASLKPDGMGEAEDDNGAVEPAYRYIETLP
                     NGVSHAIFKLRDNGRLDNTPEVTVPPDRLFVMGDNRDNSADSRVPVREGGVGLLPMDN
                     LVGRADAVVGSWDLGMRSQPVWTWLSGFRGSRFFTAVR"
     gene            5025..5288
                     /locus_tag="VGA15_26805"
     CDS             5025..5288
                     /locus_tag="VGA15_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591341.1"
                     /translation="MTFDDVRTISLAWPEVEDGTSYGTPALKVRKKLLVRLREDGDSQ
                     SAISKSGSRFCVRSRSFLLWRMILSPNRSHFGGSCADAWRLNR"
     gene            5263..5472
                     /locus_tag="VGA15_26810"
                     /pseudo
     CDS             5263..5472
                     /locus_tag="VGA15_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
     gene            5604..6176
                     /locus_tag="VGA15_26815"
     CDS             5604..6176
                     /locus_tag="VGA15_26815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX9591342.1"
                     /translation="MNTDDFIAAALRNPINEIIAEELSRLALPDAWIVAGCLVQTVWN
                     VRTARAVDYGIADYDIFYFDPDTSWQAEDAVIRKLQGQFARTGAKVEVRNQARVHLWY
                     PEKHRLPYPALHCSTQGIDRFLTKNTQVGIRRTQSCHDVYAPNGFDDIAKMIVRPNHG
                     ANFSAANYLAKAERWKKLWPEITVLPARAG"
BASE COUNT         1217 a         1981 c         2025 g         1165 t
ORIGIN      
        1 ggtcatgccc ggcgcgcgca tgttgaagat cgtggtgatg aaattgatgg cgcccagaat
       61 cgacgaagcg ccggccagat ggatcgacag gatggcaaaa tcgaccgccg gtcccggatg
      121 gcccgaggtc gacagcggcg cgtagagggt ccagcccgcg ccgacgccat tggcgcccgg
      181 ttcaccctcg acgaaggtgg agatcaacag cagcgcgaag gaagccggca gcagccagaa
      241 cgagatgttg ttcatgcgcg ggaacgccat gtcgggcgcg ccgatcatca gcggcacgaa
      301 ccagttgccg aagccgccga tcatcgccgg catcaccatg aagaagatca tgatcaggcc
      361 gtgcgaggtc acgaacacgt tgtaggtatg ggtctcgtgg aagatctgga cgccgggata
      421 catcaactcg atgcggatca tgatcgacat cgccgcgccg atgatgccgg cgatgatcgc
      481 gaacaccagg tacatcgtgc cgatgtcctt gtggttggtc gagtagacga agcgccgcca
      541 gccggtcggg tggtcgtgcg cgtggtcgtc atggacgtga tcggagtgag ccggtgttgc
      601 cgcggtcgtt gccattttca aatcctgcct tgcagtcctt ttcggacctt gcggtcccgt
      661 cgcccttgtg ccgtcgatgc gcgtgagccc gggattttac gccttgagcc tactgcgccg
      721 ctgcgcccgc ccaggcgagc gagttcgccg ggttggtcgc aaacttcttc ttcgccccgt
      781 ctacccaggt cgcgaattcc tgatcgttca ccacccgcac cgcgatcggc atgaaggcgt
      841 ggtccttgcc gcacagttcg gagcactggc cgtagaacat gccgagcttg gtggccttga
      901 accaggtttc gttcagccgg cccggtatcg agtcgatctt gatgccgaag gccgggatcg
      961 cgaaggaatg gatcacatcc gcaccggtgg tctggatccg gaccaccttg ttaataggca
     1021 cgaccatctc gttatcaacg cctagcagcc ggggctgctt gtcctgggcc agaagcgaat
     1081 cgaattcgaa cttgccgttg tcgggatagg cgtagctcca gtaccactgc ttgccggtcg
     1141 ccttgatggt cagatcggcc ttgggaatgt cgagttcctg gaacaacagc cggaacgacg
     1201 gcaccgcgat gctgaccagg atcagcaccg gaatcagggt ccaggccacc tcgatcaacg
     1261 tattgtgggt ggttctcgag gggaccggat tggatttggc gttgaacttg acgaccacga
     1321 tgaccagcag cgccagaacg aacagcgtga tgacggtgat cagcaccaac aggaaattgt
     1381 gaaaccaggt gatgttgtcc atcaccggcg tggccgcttc ctgcagcttg tattcccacg
     1441 gcgccggctg ccccagttcg gcgaacgccg ccccgccggt gagcagcgcc acgccggcca
     1501 ccgctaatcc cagcaaccgc cggcccgttt ggcccttcga catcttcatg ccgctcgcgc
     1561 tcccttgata gaaccccaaa gcattccgcc ggatggctaa aaatgctgca cgatccgccc
     1621 cctgacaaac ggccttgggt ggtactttag aagtacgcgg gccagatcgc tagaatgcgt
     1681 cgaaatccag cctctcaaac cataattctt agcctctcgc aatgcagtag tacggcgcgg
     1741 cgcgatgcgt tagcggctca tctcttggtg tcacattttc ccgtgaaatc aggcaaatgt
     1801 gccagctttg cacgctgaca gcgcttgaca ggcagatcgc ccgatgtagg cacagacggg
     1861 gcgctcgaca ggaacccgat tcgcgaacca cgcgaaccaa cgggcggccg atcccggcgc
     1921 aaagattgct tggtggtcgc cttcaaggcg ccgtcattgc ctatcagtcc attgcctgga
     1981 tcaacattgc cacgggaata tcatgggatc gacccgatga ggccttggaa acaccaggca
     2041 agaggcgacg caaaccgcag caattccctt gccgccggtt ttggccgcct gctggctgca
     2101 aacctgaccg ctgcaagtct tgctgtcgcg ctgtttttcg gcctgaccgt ggctgccagc
     2161 gcgcagggcg cggtgcggtc ggtccatggc gactggcaga tccgctgcga caccccgccc
     2221 ggcgcgcagg ccgagcaatg cgccctgatc cagagcgtgg tggcggaaga ccgctccaat
     2281 gccggcctca ccgtcatcgt gctgaaaacc gccgaccaga agagcaagct gatgcgggtg
     2341 gtggcaccct tgggcgtgct attgccttcg ggcctcggcc tgaaactcga caatcaggac
     2401 gtcggccgcg ccggttttgt cagatgcctg cccaatggct gcgtggccga ggtcgtgatg
     2461 gacgacaagc tcttgggcca gctccgcacc gccaagaccg cgacgttcat cattttcgag
     2521 acccccgaag agggcatcgg ctttcccctg agtttgaacg ggatcggcga ggggtatgac
     2581 aagctgccgt gagagattgc cgcagtcccg ggcagaccct cccctttccc ggcgatcatg
     2641 actatcttcg aagtcatcat cgtcccggcc cggtgctttc gcgcgtgagc cgggacgaca
     2701 ggccacaggt taccgtgttg taagatgacc tttcgtgagt tcttgcccaa aatactgacg
     2761 tctgtggcta tggttccccg cgctcgcggg gacgacatct gcgttgcatc ggagccttca
     2821 tgaccaatct cgccacgacc tccctgctcg accgcgccaa cctcgaccgc gatgccgtgc
     2881 gccatgaagt ggcgcgggga ctatcgggcg ccgacgacgg cgagttgttc ctggaatatg
     2941 cccagaccga agcgctgatg ttcgacaatg gccggctgaa gcaggccact tatgacacct
     3001 cgcagggttt tggactccgc gccgtcaagg atgacgcgat cggctatgcg cattcgtccg
     3061 aagtgtcgct gccggcgctg gtgcgcgccg ccgaagcggt gcaggcggtg cgcggcggct
     3121 acagcgggaa tttcgcggcg gcgccggcgc acaccaatgt gcggctttac ggcgacgaca
     3181 atccgctgga ggcgcaggac ttcgaaagca aagtaaagct gctcgccgaa atcgacgcct
     3241 atgtccgggg acgggatccg cgcgttcggc aggtcagcgt cagcgtggcg gcgacctggc
     3301 aggtggtgga aatcgtgcgc ccggacggcg agagctatcg cgacatccgc cccttggtgc
     3361 gggtcaatat ttcagtggtt gccggccagg gcgaccggca ggaaagcggc agcaaaggct
     3421 atggcggccg cgagggctac gcgcgcttca tcgagaccaa ggcgtggcgc gaggccgccg
     3481 acggcgccat ccgcgaggcg atggtcaacc ttgaatccgg ccccgcgccc gccggtgaaa
     3541 tggatgtcgt gctcggcgcc ggctggcccg gcgtgatgct gcacgaagcc gtcggccacg
     3601 ggctggaagg ggatttcaac cgcaagcaga cttccgcttt cgccgggctg atgggtcagc
     3661 aggtcgcggc caagggcgtc accgtggtcg atgacggcac catggcggcg cggcgcggct
     3721 cgctgtcgat cgacgacgag ggcacgccga ccaaccgcac cgtcctgatc gaggatggca
     3781 tcctggtcgg ctacatgcag gaccgccaga acgcccggct gatgaacatg aagccgaccg
     3841 gcaacggccg ccgccagggc tatgcccacg tgccgatgcc gcgcatgacc aacacctata
     3901 tgctcgcggg ccagcgcgat ccggcggaaa tcctggcctc ggtcaagaac ggcatctatg
     3961 ccgcgaattt cggcggcggc caggtcgaca tcacctcggg caaatatgtg ttccagtgca
     4021 ccgaggccta caagatcgag gacggcaaga tcggcgcgcc gctgaagggc gccatgctga
     4081 tcggcaacgg gccgaccgac ctgcatcgca tcaccatgat cggcaacgat ctcgcgctgg
     4141 ataccggcat cggcacctgc ggcaagaacg gccagggtgt gccggtcggc gtcggccagc
     4201 cgacactgcg gatggagcgg attaccgtgg gaggaaccgg ctgatgagca tcggaaaaga
     4261 atcggtcgcc tcaagggcgg cgggctgggg cgcgcaactt ctccagctcg cggcgatcgt
     4321 gggtatcgtt ttcgtcgcca agggtgcact cgccgagccg ttctacgtgc cgtcgggctc
     4381 catggagccc acactgctga tcggcgatgc gctgctggcg tcgaagtacc cttacggcta
     4441 cggcaccgcc tcgctgccga tccagatcaa cctgcccgag accgggcggg tgttcggcga
     4501 gccgccgaag ctcggcgatg tcgtggtgtt ccgctggccg ggcgatcact cgcaggcctg
     4561 ggtcaagcgt gtcgtcggcc tgcccggcga tcgcattcag atgcggcagg gccagctctt
     4621 catcaacgac catgcggcgt cgctgaaacc cgatgggatg ggcgaagccg aagacgacaa
     4681 cggcgcggtc gaaccggcct atcgctacat cgagaccttg ccgaacggcg tcagccacgc
     4741 gatcttcaag cttcgcgaca atggccggct cgacaacacc cccgaagtga cggtgccgcc
     4801 ggaccggctg ttcgtgatgg gcgacaaccg cgacaactcc gccgacagcc gcgtcccggt
     4861 gcgcgaaggc ggggtcgggc tattgccgat ggacaatctg gtcggccgcg ccgacgccgt
     4921 cgtcggctcc tgggatctcg gcatgcgcag ccagccggtg tggacctggc tgtcgggttt
     4981 tcgcggttcg cggttcttca ccgcggtgcg ttgatcgcgg tgccgtgacc ttcgacgacg
     5041 tcagaacgat atcgctggcg tggccggagg tcgaggacgg cacttcctat ggcacgcccg
     5101 cgctcaaggt gcgcaagaaa ttattggtgc ggctgaggga ggacggcgac agccagagcg
     5161 cgatcagcaa aagtggaagc cggttttgcg tccgatcgcg ctctttttta ttatggcgca
     5221 tgatcttgtc gccaaaccgc tcacactttg gcggatcatg cgctgatgcc tggcgtctca
     5281 atcgatgagc gcgacatgct ggtggagagc cagccgaagg tgttttactt caccgaccac
     5341 tacaaggatt atccctttgt gctgatccgg ctgtcgaaga ccaagcgcac catcgtcgag
     5401 ccgctgctgc gccggcaatg gcggacgctg gcctcaaaag cggcggtcaa agcatttgag
     5461 acaaaatcgt agggtgggca aaggcgcgct cttcgcgccg tgcccaccgc ggtcataaag
     5521 gtgtatcgat ggtgggcacg cggagtttat catcgggcgc gcgttcgcgc gacccgttgg
     5581 ctttgcccac cctacgaatt ctcatgaaca ccgacgactt catcgccgcc gcgctccgca
     5641 atcccatcaa tgaaataatt gccgaagaat tatcccggct ggcgctgcct gatgcctgga
     5701 ttgtcgccgg atgcctggtg cagaccgtgt ggaacgtacg gactgcgcgc gccgtcgact
     5761 atggcattgc cgactacgat atcttctatt tcgatcccga cacctcctgg caggccgagg
     5821 acgccgtgat ccgaaaatta cagggacagt tcgcaaggac cggcgccaag gtcgaagtgc
     5881 gcaatcaggc ccgcgtccat ctctggtatc cggaaaaaca tcggctgccc taccccgcgc
     5941 tgcattgttc gacccaaggt atcgatcgct tcctgaccaa aaatacccag gtcggaatcc
     6001 gccgtacgca atcctgccac gacgtctatg cacccaacgg ctttgacgat atcgccaaaa
     6061 tgatcgtgcg gcccaatcac ggcgcaaact tctcggcggc gaattatttg gcgaaggctg
     6121 agcgctggaa gaagctgtgg ccggaaatca cggtgctgcc ggcccgggca ggatgatgca
     6181 ttggacccct tcgtcgcttc gctcctagga agaaacaatg agttaaggca ttgattttcg
     6241 atgtgctgcg aggaaggtgc gacgaggttc ctcttcacct ctcccatggg gagaggtcgg
     6301 cgcgcagcgc cgggtgaggg gatacggtcc atcgatacac cgaaacccct cacccggatc
     6361 gcatctgacg atgcgatccg acctctcc
//
LOCUS       DASWGM010000995         6126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_804501,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000995 DASWGM010000000
VERSION     DASWGM010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804501"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VGA15_26820"
     CDS             complement(<1..283)
                     /locus_tag="VGA15_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9591343.1"
                     /translation="MRILVVGAGAIGGYFGGRMLQAGRDVTFLVRPRRAAELADAGLV
                     IKSPNGDVTLKHPPALQADKLSEKFDVVLLSCKAFDLDDAIKSFASAVGP"
     gene            complement(383..1120)
                     /locus_tag="VGA15_26825"
     CDS             complement(383..1120)
                     /locus_tag="VGA15_26825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9591344.1"
                     /translation="MEKRNATVAVIGAGDYIGTEIAKKFAAEGFTVFAGRRNGTKLEP
                     LVRDIEAAGGSIVARALDAREADEVAAFLNDADKHAPLEVCIFNVGANVSFPILETTD
                     RVFRKVWEMACWAGFVAGRESARLMLPRGKGNIFFTGATASLRGGSGFAAFASAKFGL
                     RAVAQAMARELGPKNIHVAHLVIDSGVDTAWVRERREQLWGKEALDNPDLLMPPASVA
                     ASYWQLYQQPRSAWTFELEIRPFGEKW"
     gene            complement(1178..1786)
                     /locus_tag="VGA15_26830"
     CDS             complement(1178..1786)
                     /locus_tag="VGA15_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX9591345.1"
                     /translation="MTQTSPQFMFDVGSPNAFLSHEAIPGIEQRTGVKFEYVPILLGG
                     IFKATNNKSPAETLAGIKNKPEFQVLETERFVKRFGVKPYTMNPFFPVNTLNLMRAAV
                     AAQFEGVFEKYVDAAFHHMWGEPKKMDDPEVAMKAFTASGLDAAKLFARAQEGDVKAK
                     LIENTQAAVERGAFGSPTFFVGKEMFFGKEQLREVEELVSGK"
     gene            complement(1890..3186)
                     /locus_tag="VGA15_26835"
                     /pseudo
     CDS             complement(1890..3186)
                     /locus_tag="VGA15_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131388.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(3356..3973)
                     /locus_tag="VGA15_26840"
     CDS             complement(3356..3973)
                     /locus_tag="VGA15_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HEX9591346.1"
                     /translation="MIDAAPQRFTAFIGHQRLASGPLAEVALAVLKAPTGLGAEPIVI
                     FDDATGRPIDLDLRGTEQDIVARLPKPASNAETAAEDLSAPEPRGRGRPKLGVVAREV
                     TLLPRHWDWLGAQPGGASVALRKLVEAARRASGDRDRSRAARDAAYHFMAVMAGNLAR
                     FEEASRALFADDRRRFVELIAGWPGDIRDHIVWLAFSDRAEPPKA"
     gene            4176..4841
                     /locus_tag="VGA15_26845"
     CDS             4176..4841
                     /locus_tag="VGA15_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX9591347.1"
                     /translation="MLTVHHLNNSRSQRVLWLLEELGVAYEIVRYQRQPDMRAPAELR
                     AIHPLGKSPVITDNGNTIAESGAIAEYIIDTYGNGRLIPPPKTPERVRYTYWLHYAEG
                     SAMPPLLLKLLFTLMPKRAPALLRPLVRKVSNQALTTLVNPQLKQHMAFWEGELSKRE
                     WFAGNEFTAADIQMSFPLEAAAARGGLDQGHPKAMAFLDRIHARPAYQRALEKGGPYS
                     VGR"
     gene            complement(5000..5890)
                     /locus_tag="VGA15_26850"
     CDS             complement(5000..5890)
                     /locus_tag="VGA15_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9591348.1"
                     /translation="MTTLKGKTLFVSGGSRGIGLAIALRAARDGANVAIAAKTAEPHP
                     KLKGTIYTAADEIRAAGGKALPILCDIREEAQVMAAIDRTVAEFGGIDICVNNASAIS
                     LTNSQATDMKRFDLMMGINTRGTFMVSKYCIPHLEKSKNPHILMLSPPLDMKAKWFEH
                     STAYTMAKFGMSMCVLGLAAELKGSGVAVNALWPRTTIATAAVGNLLGGDAMMRASRK
                     PEIMGDAAHVIFTKPSREFTGQFCIDDKVLYASGVRAFDHYRVDQSVPLMSDFFVPDD
                     DVPPPGVTVRALPSVGQVQK"
BASE COUNT         1173 a         1934 c         1914 g         1105 t
ORIGIN      
        1 gcggtccaac cgctgatgca aacgacttga tcgcatcgtc gaggtcgaac gccttgcagc
       61 tcagcaagac cacgtcgaat ttttcgctca atttgtcggc ctgcaatgcc ggcggatgtt
      121 tcagcgtcac gtcgccattc gggcttttga tcacaaggcc ggcgtcagcc agctctgccg
      181 cgcgcctggg gcggaccaga aaggtcacat cgcggccagc ctgcagcatc ctgccgccga
      241 aatatccgcc gatggcgccg gcaccgacca cgagaatacg cattgctgag ttccttttgt
      301 tgtttgttct tgaagcggcg aatagggagt ggcgaatggc gaatagggaa atttccattc
      361 gctattcgcc attcgcgtcc tcttaccact tttccccgaa cgggcggatc tccagctcaa
      421 aggtccaggc gctgcgcggc tgctggtaaa gctgccaata ggacgccgcg accgacgccg
      481 gcggcatcag caagtccgga ttgtcgagcg cttccttgcc ccagagttgc tcccggcgct
      541 cgcgcaccca ggcggtgtcg acgccggaat cgatgaccag atgcgcgaca tggatgttct
      601 tcggcccgag ttcgcgcgcc atcgcctgcg ccaccgcccg caagccgaac ttggcgctgg
      661 caaaggccgc aaagccgcta ccgccgcgca agctcgcggt cgcgccagtg aagaagatgt
      721 tgcccttgcc ccgcggcagc atcagccgcg ccgattcgcg cccggcgacg aagcctgccc
      781 agcaggccat ttcccagacc ttgcggaaga cgcggtcggt ggtctccaga atgggaaagc
      841 tgacgttggc gccgacattg aagatgcaga cctccagcgg cgcatgcttg tcggcgtcat
      901 tgaggaacgc cgccacctcg tccgcctcgc gcgcgtcgag cgcgcgcgcg acgatcgatc
      961 cgcccgcggc ctcgatatcc cgaaccagcg gctcgagctt ggtgccgttg cgacgcccgg
     1021 cgaatacggt gaatccctcg gcggcgaatt tcttggcgat ttcggtgccg atataatcgc
     1081 cggcgccgat caccgcgacg gttgcgttgc gtttttccat tctgggttct ccaccaggcc
     1141 tgacgagcct acccgccctc ctcgcaatga cggcaagtca cttacccgaa accaactcct
     1201 cgacctcgcg caattgctcc ttgccgaaaa acatttcctt gccgacgaag aaggtcggcg
     1261 agccgaaggc gccgcgctcg accgccgctt gcgtgttctc gatcagcttc gccttgacgt
     1321 ccccttcttg cgcgcgggcg aacagttttg ctgcgtcgag gccggatgcc gtgaacgctt
     1381 tcatcgccac ttcgggatcg tccatttttt tcggctcgcc ccacatgtga tgaaacgcgg
     1441 catcgacata tttctcaaac acgccctcga actgggctgc gaccgcggcg cgcattaaat
     1501 tgagggtgtt gaccggaaag aacgggttca tcgtgtaggg cttgaccccg aaacgcttta
     1561 cgaaccgctc ggtctccaac acctgaaact cgggcttgtt cttgatgccg gcaagtgttt
     1621 cggcgggaga cttgttgttg gtggccttga agatgccgcc gagcagaatc ggcacatatt
     1681 cgaatttcac gccggtgcgt tgttcgattc ccgggatggc ctcgtgactg agaaaggcat
     1741 tggggctgcc gacatcgaac atgaattgcg gcgacgtctg ggtcaaggcg gcctcccggg
     1801 cttttctgct accgccgtat tctggcgcag cacgcccggc gcgtccactg tattcatgac
     1861 gaccataata ctctgcgggc gaggcacggt cagatcaacc gtcgtatcgc gcgtttgcgc
     1921 cataccagca tatagtagct catctgcaga atgaacatgg cggtaaaggt gatcggatag
     1981 gcggcccaga cgccttcgat gccgatggcg cggctgagga tgatcgcggc cggcacctcg
     2041 atcgccgcaa tcacaaaaat cgagatcagc aacggcatca acaccgttcc gctggcgcgc
     2101 atcatgcccg agaacacggt cgccatgccg aacagcaccg aactccacaa cacgatgtgg
     2161 agcaatccct gcgcgagatc gagcaccgcc gcgtcgataa tgaagaagct catgatggcg
     2221 cgtgagaaca ggtaggcgag cgccaccaat ccgccggtca gaacgaggtt catcacgagc
     2281 ccggtgcgga caatggaacc aagccggtcg gcgttgccgc ggccgatcgc ctgagcgccg
     2341 aagatcgaga ccgaaatcgc gatcgacatc gccggaaact gcacgtagct catgacctgg
     2401 ttgaccgcgc cataggctgc cgtggcgctg gagccaaagc cgttgacgag gccgagcagg
     2461 accagctccg ccagcgacat cacgaccatt ccgacggcgg cggggatacc cagacgcagg
     2521 accttgccga gcagcgcgcc gtccagccgc atgctgcgca gaaacgccgc gtcaaaggca
     2581 aggggatgat ggcggcgacg aaggtttacg gcgagccaca gcagggtgag caggctcgat
     2641 gccgccgacg cccaggcggc gctggcgacg ccgaaccgag gtaagcccaa ccagccccga
     2701 atcaacgccg gggttacggc aagaccgacg gttgtcgaga tcgtcagcgc cagcagcggc
     2761 gtcatggtgt cgccgacacc acgcaacatt gccgtcagca gaatgaagac aaaggtcagc
     2821 ggcatggtga tcatcatgat gcgcataggc gatggcctcc tcgagaatgt cgggcggcgt
     2881 ggcgagcgcg atgaggagcg gccggctgaa cacgccgccg aacacggcaa tcgttgtcgc
     2941 gcagagcagc gcgaccgtca gcgtcgcgcc ggcgacggcc ctcaccttgc cgggttcgcc
     3001 cgcaccccag gcctggccga tcaggacggt ggcgcccgag ctcagcccca taacgaaggc
     3061 gacaaaaaag aacatcaccg ggaaaaacac cgagacggcc gcgagcgcat cgaccccgat
     3121 catctggccg aggtacacgt tgttgatggt gccgaacaac gcctgcagaa tattgctcag
     3181 catcatcggc gcgaggaaaa ccagaaatgt tttccagagg gaagggttcg acacggggtt
     3241 tccttattgg tcggcgtgac cggtcgaggc cacgcaacgt tgttgtgtcg gaggtgactg
     3301 ctttagagag cgggctggcg gccggtgatg ccttggatgt tcaaggcttg cgcgttcagg
     3361 ctttgggcgg ttcggcgcga tcgctgaagg cgagccagac gatgtggtcg cggatatcgc
     3421 caggccagcc ggcgatgagc tcgacaaaac gtcgtcggtc gtccgcgaac aaggcccgcg
     3481 aggcttcctc gaaacgcgcg agattgccgg ccatgacagc catgaaatga taggcggcgt
     3541 ctctggcggc gcggctgcga tcgcggtcgc cgctggcgcg acgtgccgcc tcgaccagct
     3601 ttcgcaacgc caccgaggcg cctccgggct gtgccccgag ccaatcccag tgccgcggca
     3661 acagcgtcac ctcgcgggcg acgacgccga gtttgggccg cccgcggcca cgcggctccg
     3721 gcgcagacag gtcttcggca gcagtttccg cattcgaagc cggcttgggc aaacgcgcga
     3781 cgatatcttg ctcggtgccg cggagatcca gatcgatggg ccggccggtc gcatcgtcaa
     3841 agatgacgat aggctccgcg cccaggcctg tgggcgcctt cagcacggcc agcgcgacct
     3901 cggccagcgg gcctgaagcc agccgctggt gacctatgaa agcggtgaag cgctggggag
     3961 ctgcgtcgat catcgatcaa tactctgaat tcctgtagcg tttgatttat acccgggtaa
     4021 atttagatgt caatatcacc cgggtataat atcctggagc cggtccagcc ccatcgcgtg
     4081 gcgaagcccg aaccaccgtc caatcgccaa cctcgccgcc tgctgctacg aggtcagcac
     4141 atctaacgtc gccaaagtgc ccgggggaat tcgccatgct gaccgtccac catctcaaca
     4201 actcccgctc tcaacgcgtg ctgtggttgc tggaggaact cggcgtggcc tacgagatcg
     4261 tccgctatca gcgtcagccg gacatgcgtg ccccggcgga gttgcgcgcg atccatccgc
     4321 tcggaaaatc gccagtcatt accgacaatg gtaacaccat cgccgaatca ggcgcgatcg
     4381 ccgaatacat catcgatact tacggcaacg gccggctgat cccgccgccg aaaacgccgg
     4441 agcgggtgcg ctatacctat tggctgcatt acgccgaagg ctccgcgatg ccgccgctgc
     4501 tgctgaaatt gttgtttacg ctgatgccga agcgcgcgcc ggcgctgctg cggccgctgg
     4561 tgcgcaaggt ctccaatcag gcgctgacca cgctggtcaa tccgcagctc aagcagcaca
     4621 tggcgttctg ggaaggcgag ctttccaagc gcgaatggtt cgccggcaac gaattcaccg
     4681 ccgccgacat ccagatgagc tttccgcttg aagccgccgc cgcgcgcggc gggctcgatc
     4741 agggccaccc caaggcgatg gcattcctgg accgcattca cgcccgcccg gcttaccagc
     4801 gcgcgcttga aaagggtgga ccgtattcgg tggggcggta gaaactcgtc atcctgaggt
     4861 gctcgccgtc ttcggcgggc ctcgaaggat tggctacgtc atcgccgtcg cccatcgagg
     4921 cgcgcaagga agagcgcgca cctcagggtg acggctacgg ctgtaaggca cgcgccccga
     4981 atcggcagcg cttttagtgc tacttctgca cctggccgac cgaaggcagc gccctcacgg
     5041 tgaccccggg cggcggcacg tcgtcgtcgg gcacaaagaa atccgacatc aggggcacgg
     5101 attgatcgac gcggtagtgg tcgaaggcgc gcacgccgct ggcgtagagc accttgtcgt
     5161 cgatgcaaaa ttgcccggtg aattcccgcg acggctttgt gaatatgaca tgggcggcgt
     5221 cgcccatgat ctcgggtttg cggctggcgc gcatcatggc gtcgccccct aagagattgc
     5281 cgaccgcggc ggtggcaatg gtggtgcgcg gccacagcgc gttgacggca acgcctgagc
     5341 ccttcagttc ggccgcgaga ccgagcacgc acatgctcat gccgaacttc gccatggtgt
     5401 aggcggtgga gtgctcgaac catttcgctt tcatgtcgag tggcggcgac agcatcagga
     5461 tgtgcgggtt tttcgatttc tccagatgcg ggatgcagta tttcgacacc atgaaggtgc
     5521 cgcgggtgtt gatgcccatc atcaggtcga agcgcttcat gtcggtcgct tgcgagttgg
     5581 tcaggctgat ggcgctggcg ttgttgacgc agatatcgat gccgccgaat tcggcgacgg
     5641 ttctatcgat cgccgccatc acctgggctt cctcgcggat atcgcaaagg atcggcagcg
     5701 ccttgccgcc ggcggcgcgg atctcgtcgg cggcggtgta gatcgtgccc ttgagtttcg
     5761 gatgcggctc ggcggttttc gcggcgatcg cgacattggc gccgtcgcgc gcggcacgca
     5821 acgcgatcgc aagcccgatg ccgcggctgc cgccggatac gaacagcgtc ttgcccttga
     5881 gtgtggtcat cgaaacagct ccaaatcgat acggaacgcg tgagtttcgt acctctccta
     5941 ccgggaggga gcacgctccg gcagattcat gtcacgacaa aatgcaagaa ggctggtgtg
     6001 ttcgatcgct gcgatttcga tcgcccgcgc tggcccggcc gtactcataa tggatgttcc
     6061 cccggcgaat tctgaccgac ctttcggtcc gccccgcgtt ggccggttgg cgcggaggat
     6121 acccaa
//
LOCUS       DASWGM010000996         8871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_804533,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000996 DASWGM010000000
VERSION     DASWGM010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804533"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VGA15_26855"
     CDS             <1..201
                     /locus_tag="VGA15_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591349.1"
                     /translation="AEVNTMADLAVHPHLRRIEVDTPAGPVTYPAPAAIVVGQPRHYG
                     AVPGIGDHTDIAKSFHVRTKSS"
     gene            198..1046
                     /locus_tag="VGA15_26860"
     CDS             198..1046
                     /locus_tag="VGA15_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX9591350.1"
                     /translation="MTDKPDLDHLRQWIGRTEEASDIVTAQLVKGLRATLFMEIGKPK
                     PGDATPWTVHWCLAPPVYPMSALSADGHPTRGGFLPPVPLPRRMWAGGELEFFEPLRV
                     GDETTRTSRIADVTMKTGSTGVLCFVSVEHLVTTPRGLAIRERQDIVYRDISTAPAAA
                     KPAVAPPSAQHRESHMADPVLLFRYSALTFNGHRIHYDRDYVTKVEGYPGLIFHGPMQ
                     AAFLVEFAAKLHGGAAPKKFSYRGVLPLFEGSEFSVNANESNAGMELWTANSQGQPTM
                     KGTATW"
     gene            1300..2928
                     /gene="mdlC"
                     /locus_tag="VGA15_26865"
     CDS             1300..2928
                     /gene="mdlC"
                     /locus_tag="VGA15_26865"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HEX9591351.1"
                     /translation="MPPREKPKATGTSVTVKHATLNLLRAFGIKKVFGNPGSTELPFL
                     ADWPDDIDYVLGLQEASVIGMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAHRNQT
                     PLVITAGQQARSILPLQAFLYAERASEFPRPYVKFSVEPARAEDVPAAIARAYYVAMQ
                     PPCGPTFVSVPIDDWTHQTRAIEARRVSRELGPDPEAMKSLVAALSASKRPALVVGPG
                     VDRAHAVDLMVRVAEKAKAVVWVSPFSARCSFPERHPLFAGFLHASPGQLSDALRNYD
                     LVVVIGAPVFTFHVEGHAAIFDGATALFQITDDPTAAAVSPQGNSIIATMKPALATLL
                     ELLPETRRAMPAGRLLPSAPVAADPIPVEFLLHALFSAMPDDAALVEEAPSHRPAMQK
                     FMPMRGQDSFYTMASGGLGYSLPASVGVALGRPDIRTVCLIGDGSAMYSIQALWTAAQ
                     RKLPLTVVVINNAGYGAMRSFSQVMQVRNVPGLDLPGIDFVKIAEGMGCEAVRVSKSS
                     ELAPALKRGLAHDGVSLIEVMVDSAVPLLYAQKG"
     gene            complement(3192..3986)
                     /locus_tag="VGA15_26870"
     CDS             complement(3192..3986)
                     /locus_tag="VGA15_26870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX9591352.1"
                     /translation="MQVAGKVVVVTGGANGIGRALCEALHRAGAAKVVVADLDLAGAE
                     AVAASVHGAAFKCDVGREKDILNVIEETERQFGPIALFCSNAGIGGGFDPLSENAGGT
                     SDEPFARSWAVHVMAHIYAARHLIPRMKARGEGYFLNTISAAGLLSQVGSPAYSTTKH
                     AAVGFAENLAISHKADGIKVSILCPQGVDTNMLRSIPKGPQSGDGDLTPEQVANDALA
                     GLEQETFLILPHPQVLGYMRKKTENYDRWISGMAKIQAKMREAYGK"
     gene            complement(4012..4707)
                     /locus_tag="VGA15_26875"
     CDS             complement(4012..4707)
                     /locus_tag="VGA15_26875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX9591353.1"
                     /translation="MTIFRLKQLASTSAHAVAGAVRWNYDRAELIADGIVHGIGVFGG
                     LIAATVLVVLTAVYATGFEVAVVSVYVAGLLAMLVLSATYNLWPISRAKWVLRRFDHS
                     AIYVLIAATYTPFITQMKDSIFAIALLTGVWCVAIVGIVIKLVFPGRFDRLAVGLYLA
                     MGWSGFMVYDTVVASLPPLTLWFIAAGGVLYTFGVVFHAWQRLRFQNAIWHAFVLLGA
                     ACHYTAVLDLVLT"
     gene            4890..6359
                     /locus_tag="VGA15_26880"
     CDS             4890..6359
                     /locus_tag="VGA15_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HEX9591354.1"
                     /translation="MALSFSEIVEEARLSARALLDYGDHFFNPTVRLGVTGLSRAGKT
                     VFITALIHGLTRGGRFPIFEPFATGRIARARLAPQPDDAVPRFDYENHVRTLIEQRRW
                     PNSTVDISELRLVIDYQRQNGADRTLTLDIVDYPGEWLLDLPLLNKSFEQWSAESLAL
                     SQESPRARLAEPWHAHLATLSASGPEDEQAALAAARLFTEYLRACRDERFAMSLLPPG
                     RFLMPGNLAGSPALTFAPLDLPDDGAAPEGSLWAMMRRRYEAYKDVVVRPFFRDHFSR
                     LDRQVVLVDALSAFNSGPEALHDLEAALAGILDCFRIGRSTLLSSLFRPRIDRILFAA
                     TKADHLHHSSHDRLEAVLRRILNRAVAKAEDTGADIDVVALAAVRATREAQVQRGREK
                     LPSILGTPASGEIAGGEAFDGETEVATFPGDLPADPEELFKGDSAFQGLSSATAEKSD
                     FRFLRFRPPHLDSQNADGPMLPHIRLDRALQFLIGDRLQ"
     gene            6356..7387
                     /locus_tag="VGA15_26885"
     CDS             6356..7387
                     /locus_tag="VGA15_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HEX9591355.1"
                     /translation="MSERPRHRKPATFKLDDPGVTVIDPDDSSRPSRGTIHITPELEP
                     ALLPVPVEAPIVAARRGFRWGAVFWAAVGGLILLGLGLSTTRLIEDLFARSEGLGYLG
                     LAFAFAAALALVVVVAREAFGLARLATIEKMHLRAAAALLSDDRAESRTIVQDLLKLA
                     HQNPQLARARATLQGHADDIIDGADLIRLAERELMTPLDQEARRLISSAAQRVSIVTA
                     VSPRALIDVLFVFAASLRLIRQLARLYGGRPGALGMIRLMRHVIAHVAITGGMAASDS
                     LVQQVLGHGIAAKLSQRLGEGVLNGLLTARLGLAAIDVTRPLPFTALPRPALGDLAKD
                     LLRKREAEE"
     gene            complement(7485..7772)
                     /locus_tag="VGA15_26890"
     CDS             complement(7485..7772)
                     /locus_tag="VGA15_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591356.1"
                     /translation="MGKIASAIVAATLATAPLVLPAPARAENGQIAAGVVGGLIGGAL
                     LGGALAARPAPPPPAYYAPEPVYVDEPVCRLVRERYWDGYDWRIRRVQVCN"
     gene            complement(7878..8816)
                     /locus_tag="VGA15_26895"
     CDS             complement(7878..8816)
                     /locus_tag="VGA15_26895"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX9591357.1"
                     /translation="MRLATFVDPQLGREARFGIVRGESVVDVVAAANALHRAVPAMSV
                     KVALTSGSQMLAALEELAGSAEREKLLRPIAGLKFLPPIPDPSKFFCVGKNNKKHREE
                     LAANKMLTEVPNEPTGFIKLTSTISGDGDEVVKPAGITTLDYEPELCYVVGKRAHGVK
                     KTEAMDYIAGFTLTNDISAREIQKREVASGSRFWTAKNMPGFAPVGPHILTMEEVADT
                     DNLWVTCDVNGKRRLRANTGDYLYKIADVLEHFSRYVVFEPGDLIAMGAPSGVAVGQP
                     NAAELYLQPGDDMVISLEGLMSLQTRIVAPAGASRK"
BASE COUNT         1633 a         2873 c         2707 g         1658 t
ORIGIN      
        1 gccgaggtca acaccatggc tgaccttgcg gtgcacccgc atctgcgccg catcgaagtg
       61 gacacgccgg cgggaccggt gacctatccg gcgccggctg ccatcgtcgt cggtcaaccg
      121 cgccactatg gcgccgtgcc cggcatcggc gatcacaccg acatcgccaa atcttttcac
      181 gtccggacca aatcgtcatg accgacaaac ccgatctcga ccatttgcgg caatggatcg
      241 gccgcaccga agaagcctcc gacatcgtca ccgcgcaact ggtgaagggt ttgcgcgcga
      301 ctttgttcat ggagatcggt aagccgaaac cgggcgacgc gacgccgtgg acggtgcatt
      361 ggtgcctggc gccgccggtc tacccgatgt cggcattgag tgcggacggc caccccaccc
      421 gcggcggctt cctgcccccg gtgccgctgc cgcgccggat gtgggccggc ggtgagcttg
      481 aattcttcga accactgcgt gtcggcgacg agacgacgcg aacctcacgc attgccgatg
      541 tgaccatgaa gaccggcagc accggtgtgc tgtgtttcgt atccgtcgaa catctggtca
      601 ccacgccgcg cgggttggcc atccgcgagc ggcaggatat cgtctatcgg gatatctcga
      661 ccgcgccggc agcagccaag cccgccgtgg cgccaccgtc ggcgcagcat cgcgaaagcc
      721 acatggccga tccggtgctg ctgtttcgtt attcggcgct gaccttcaat ggccaccgca
      781 tccattacga ccgcgactac gtcaccaagg tcgagggcta tcccggcctg atcttccacg
      841 gcccgatgca ggcggcgttt ctggtggagt tcgcggcgaa gctgcatggc ggcgcggccc
      901 cgaagaaatt cagctatcgc ggggtgctgc cgctgttcga aggcagcgag ttcagcgtca
      961 atgccaacga atccaatgcc ggcatggaac tgtggacggc gaattcacag gggcagccga
     1021 ccatgaaagg cactgcgacg tggtgaggtt cgccagtctc gcgtcaacgg gaaatctgct
     1081 ccctctccca cccaactcgg gtttacccga gttgggcatc taaatcggtc gaagtcggat
     1141 atatccgact tcgatgggag agggctgggg tgaggggtta cggtctatcg ttaggccagt
     1201 tcccctcacc cggcgcttcg cgccgacctc tccccatggg agaggtgaaa ccgaactcgt
     1261 agcacgtttt gccgcaaaca ctcaccgaag gatcgtcagt tgccgccgcg tgaaaaaccc
     1321 aaagcgacag gcacttcggt taccgtcaag cacgcaacgc tcaacctgtt gcgcgccttc
     1381 ggaatcaaaa aggtgttcgg caatcccggc tcgacggaat tgccgttcct cgccgactgg
     1441 cctgacgata tcgactatgt gctgggcttg caggaagcat ccgtgatcgg catggccgac
     1501 ggctatgcgc aggccacccg caatgccggt ttcgtcaatc ttcattcggc ggccggcgtc
     1561 ggcaatgcgc tcggcaacat ctataccgcg catcggaacc agaccccgct ggtgattacc
     1621 gcgggccagc aggcgcgcag cattctgccg ctgcaggcgt tcctgtacgc cgaacgcgcg
     1681 tcggaatttc cacggcctta tgtcaaattc agcgtcgagc ctgcgcgtgc cgaagacgtg
     1741 ccggcggcga tcgcgcgagc ctactacgtg gcgatgcagc cgccctgcgg accgactttt
     1801 gtttccgtgc cgatcgacga ctggactcat caaacccggg cgatagaagc ccgtcgcgtc
     1861 agcagggagc tcggccccga tcccgaggcg atgaagtcgc tggtcgctgc actttcggca
     1921 agcaagcgcc ctgccctcgt cgtcggcccc ggcgtcgatc gcgcgcacgc ggtcgatctg
     1981 atggtgcggg tcgcggagaa ggccaaggct gtggtctggg tcagcccgtt ttccgcgcgc
     2041 tgcagtttcc cggagcggca tccgctattc gcaggctttt tgcatgcttc tccaggtcag
     2101 ctctcggacg cgttgcgtaa ctatgatctg gtggtcgtga tcggcgcgcc ggtgtttacg
     2161 ttccacgtcg aaggccacgc cgcgatcttc gacggcgcga ccgcgctgtt ccagatcacc
     2221 gacgatccca cggcggctgc ggtatcgccg cagggcaaca gcattatcgc gaccatgaag
     2281 cccgcgctgg cgacgctgct cgagctgctg cctgagacga ggcgcgcgat gccggcgggc
     2341 cggcttctgc catccgcgcc ggtcgccgcc gatccgatac cggttgaatt cctgttgcac
     2401 gcgctattca gcgccatgcc tgacgacgcc gcgctggtcg aggaagcccc gtcgcatcgc
     2461 cccgcgatgc aaaagttcat gccgatgcgc ggccaggaca gtttttacac catggcgagc
     2521 ggcggcctcg ggtacagcct gcccgcctcg gtcggcgtgg cgctcggccg tcccgacatc
     2581 aggaccgtct gcctgatcgg cgacggctcg gcgatgtatt cgatccaggc gctatggacc
     2641 gcggcgcaac gaaaacttcc gctgacggtg gtggtgatca ataacgccgg ctacggcgcg
     2701 atgcgctcgt tcagccaggt gatgcaggta cgcaacgttc ccggcctcga ccttcccggc
     2761 atcgattttg tgaagatcgc ggaaggcatg ggctgcgaag cggtgcgcgt cagcaagtca
     2821 tcggaattgg cgccggcgct caagcgcggc cttgcgcatg acggcgtcag tttgattgaa
     2881 gtgatggtgg attcggcggt gccgctgctc tacgcgcaga agggctgacg ggaacgcccc
     2941 acacgtcatt gcgagcgaag cgaagcaatc catagggcgg caaaggaaga gtggattgct
     3001 tcgtcgcgaa gcctgtcatc gggcgcgcat tcgcgcgacc cgttggctcc taggaagaaa
     3061 gtgggaaaac tccccgagcc ctatcgcctt gattcaccac actttcatcc atcagggtcg
     3121 gccccaaggc cggtacggaa ctcgcaatga cggtttgcga gcgagccgcg ttcctcacca
     3181 tgatgggccc tttacttccc gtaggcttcc cgcatcttgg cctggatctt cgccatgcct
     3241 gatatccagc gatcgtaatt ttcggtcttc ttgcgcatgt agcccaagac ttgcggatgc
     3301 ggcaggatca agaaagtttc ctgctctagt ccggccaacg catcgttcgc gacttgctcc
     3361 ggcgtcagat cgccatcgcc ggattgcggc cccttcggga tcgagcggag catgttggta
     3421 tcgacgccct gcgggcacag gatcgaaacc ttgatgccgt cggccttgtg cgagatcgca
     3481 aggttctccg caaatcccac cgccgcatgc ttggtcgtcg aataggccgg acttcccacc
     3541 tgcgacagca gtccggcggc ggaaatcgtg ttgaggaaat acccctcccc gcgcgccttc
     3601 atgcgcggga tcagatgccg cgccgcatag atgtgcgcca tgacatgaac agcccagctc
     3661 ctcgcaaacg gctcgtcgga cgtgccgccg gcgttttccg ataacggatc gaacccgccg
     3721 ccgatgccgg cattggaaca gaacagcgcg atcggaccga actgccgttc ggtttcctcg
     3781 atgacgttga gaatatcctt ctcccgtccg acatcgcatt tgaacgccgc gccatgaacc
     3841 gaggcggcga cggcctccgc acccgccaga tccaggtcgg cgacgaccac ctttgccgca
     3901 ccggcacgat gaagggcttc gcagagcgcc cggccgatgc cgttggcgcc tccggtgaca
     3961 accacgactt tgcctgccac ctgcatcgtt acacccgttg tttgaaaagt ctcaggtcaa
     4021 aacgagatcg agcacggcgg tgtaatggca ggccgcgccg agcagcacga aggcatgcca
     4081 gatcgcgttt tgaaaacgca gccgctgcca ggcatgaaag acaacgccga aggtgtagag
     4141 cacgcctccg gccgcgatga accacaatgt cagaggcggc agcgacgcca ccacggtgtc
     4201 gtagaccatg aagccgctcc agcccatcgc cagatagaga ccgaccgcca gccggtcgaa
     4261 acggcccggg aacacgagct tgatcacgat cccgacaatc gccacgcacc acacgccggt
     4321 cagcagcgcg atggcaaaaa tgctgtcctt catctgggtg atgaacgggg tgtaggttgc
     4381 cgcgatcagc acatagatcg ccgagtgatc gaagcggcgc agcacccact tggcccgcga
     4441 gatcggccaa agattatagg ttgcggacaa caccagcatg gccagcaatc ccgcgacgta
     4501 aaccgacacc accgcgactt caaaccccgt ggcataaacc gcggtgagca cgaccagcac
     4561 ggtcgctgcg atcaatccgc cgaacacgcc aatgccatgc acgataccgt ccgcgatcag
     4621 ctcggcccga tcgtaattcc agcggacggc gccagccacc gcatgggcgg acgtcgaagc
     4681 gagttgctta agcctgaaaa tggtcatagc ggtcccggcg aattcatacg cgaagcgata
     4741 aaacataggt ctcgcgaggt caacgaacgt tcaaatccct cgctgtaatc gcgtccgaaa
     4801 tgtgaaactt gattttggcg cgctaaccgc cactttacag gttgttctgc agccaatttc
     4861 ccccaccaga tcgagttttt caagcccgca tggccctcag cttttccgaa atcgtcgaag
     4921 aagcgcggct ttcggcgcgg gccctgctgg attatggcga tcactttttc aatccaacgg
     4981 tgcggctggg cgtcaccggg ctgtcacgcg ccggcaaaac cgtctttatc acggcgctga
     5041 ttcacggcct cacccgtggc ggccgctttc ccatctttga gcctttcgcc acgggacgga
     5101 tcgctcgcgc ccggcttgcg ccgcagcccg acgatgcggt gccgcgtttc gactatgaaa
     5161 accatgtccg caccttgatc gagcagcgcc gctggccgaa ttcgacggtc gatatcagcg
     5221 agcttcggct ggtcatcgac taccagcgac aaaatggcgc ggaccgcacg ctgacgctcg
     5281 acatcgtcga ttatcccggc gagtggctgc tcgatctgcc gctgctcaac aagagttttg
     5341 agcaatggtc cgccgagagc ctcgctctgt cacaagaaag tccgcgtgcg cggctcgctg
     5401 agccatggca cgcgcatctt gcgacactga gcgcctcagg ccccgaagac gagcaggccg
     5461 cgctggctgc cgcgcggctg ttcaccgaat atttgcgcgc ctgccgcgac gaacgctttg
     5521 ccatgagcct gttgccgccc ggccgcttcc tgatgccggg aaacctcgca ggctcgccgg
     5581 cgctgacctt tgcgccgctc gatctgcctg acgatggcgc ggcgccagag ggttcgctgt
     5641 gggcgatgat gcggcggcgt tacgaggcct acaaggacgt ggtggttcgc ccgttctttc
     5701 gcgatcattt ttcaaggctc gatcgccagg tggtgctggt cgatgcgctc tcggcgttca
     5761 actcagggcc ggaagcgctg cacgatctgg aagcagcact ggcgggaatt ctggattgct
     5821 tcaggatcgg ccgcagtacg ctgctcagca gtttgttccg gccgcggatc gaccggatct
     5881 tgttcgccgc gaccaaggcc gatcacctgc accattccag ccatgatcgt ctcgaggcgg
     5941 tgctgcggcg aatcctcaac agggctgtcg caaaagccga ggacactggc gccgatatcg
     6001 acgtcgttgc gctggccgcg gtgcgcgcca cccgcgaggc ccaggtgcag cgcggccgtg
     6061 aaaaactgcc ctcgatcctg ggcacccctg catcaggcga aatagcgggt ggcgaggcct
     6121 tcgacggcga gaccgaggtc gcgacatttc ccggcgatct gccggccgat cccgaagagc
     6181 tcttcaaggg cgacagcgcg tttcagggcc tttccagcgc gaccgcggaa aagagcgatt
     6241 ttcgctttct tcgctttcgg ccgccgcatc tcgatagcca aaatgcggac ggtcccatgt
     6301 tgcctcacat ccgcctcgac cgcgccctcc agttcctgat cggagacaga ctgcaatgag
     6361 cgagcgcccc cgtcatcgca agccggcgac cttcaagctc gacgatcccg gcgtgaccgt
     6421 gatcgatccg gacgattcaa gccgcccatc gcgcggcacg atccacatca cgccggaact
     6481 ggagcctgcg ctgctgccgg tgccggttga ggcgccgatc gttgccgcgc gacgaggttt
     6541 tcgctggggc gcggtgttct gggcggcggt cggcggattg atcctgctcg ggctcgggct
     6601 gagcaccacg cgtttgatcg aggatctgtt cgcgcgaagc gaggggctcg gctatctcgg
     6661 attggcattt gcctttgctg ccgcgctcgc ccttgtcgtc gtcgtcgcgc gagaggcgtt
     6721 cggtctggcg cgacttgcaa ccattgaaaa aatgcatctg cgcgctgccg cggcactgct
     6781 cagcgatgat cgtgcggaaa gccggaccat cgtgcaggat ctgttaaaac ttgcacacca
     6841 gaacccgcag ctggcgcggg cccgcgccac gctgcagggc catgccgacg acatcatcga
     6901 cggcgccgat ctgattcgcc tcgcggaacg cgagttgatg acgccgctcg atcaggaggc
     6961 gcgccgcctg atctcgtcgg cggcgcagcg cgtctcgatc gtcaccgccg tcagcccgcg
     7021 cgccttgatc gacgtgctgt tcgtcttcgc ggcatcgctg cgcctgatcc ggcagttggc
     7081 gcgcctgtat ggcggacggc cgggcgcgct cggcatgatc cggctgatgc gtcatgtcat
     7141 tgcgcatgtc gccattaccg gtggcatggc ggcaagcgac agcctggtcc agcaggtgct
     7201 cggccatggc atcgcagcaa aactgtcgca gcggctcggc gaaggcgtgc tcaacggact
     7261 gctcaccgcg cggcttgggc ttgcggccat cgacgtgacg cgtccgctgc ccttcaccgc
     7321 gttgccgcgg ccggcgctcg gcgatctcgc caaggacctc ttgcgcaagc gcgaggccga
     7381 ggagtgagct ctaatttggc gcgacgatta ggccagacaa aagaccgccc cgagggcggc
     7441 cggatttgag ttcgttcaag ccgcaaccgc ggaggagtgc gcgatcagtt gcaaacctgg
     7501 acccggcgaa ttcgccagtc atatccatcc cagtagcgct cgcgcaccaa acggcaaact
     7561 ggttcatcga cgtacaccgg ctccggcgcg taataagccg gcggcggagg cgcaggacgt
     7621 gctgccagtg cgccgccgag cagcgccccg ccgataagtc cgccgacgac gccagcggcg
     7681 atttgaccgt tctccgctct tgcgggcgcc ggcaacacaa gcggagccgt tgccaaggta
     7741 gcagccacga tagctgaagc gattttgccc atcaggtttc tccaagtcgc agccgcaaat
     7801 cgttcacgga ccgccacaac cgaccatcat acatttcgtg attaacgctt tcttaagtct
     7861 gcgcgcaagc gggtaaatta ttttctggaa gcgccagccg gcgcgacgat ccgtgtttgc
     7921 agcgacatca gcccttccaa cgagatcacc atgtcgtcac cgggctgcaa atagagttcg
     7981 gccgcattgg gctgccctac cgcgacaccg ctcggcgccc ccatggcgat caggtcgccg
     8041 ggctcgaaca cgacataacg cgaaaaatgc tccagcacgt cggcgatctt gtagagataa
     8101 tcgccggtgt tcgcgcgcag ccgccgcttg ccgttgacgt cgcaggtgac ccacagattg
     8161 tcggtatcgg cgacttcctc catggtcaga atgtgcggac cgaccggcgc gaagccgggc
     8221 atgtttttcg cggtccagaa ccggctgccg gaggccacct cacgcttctg gatttcccgc
     8281 gcgctgatat cgttggtgag cgtgaagccc gctatatagt ccatcgcctc ggtcttcttg
     8341 acgccgtggg cgcgcttgcc gacgacgtaa caaagctccg gctcgtaatc gagcgtggta
     8401 atgccggctg gcttgacgac ctcgtcgcca tcacctgata tcgtcgaggt gagcttgatg
     8461 aacccggtcg gctcgttcgg gacttcagtc agcatcttgt tggcggcgag ttcctcgcga
     8521 tgttttttgt tgttcttgcc tacgcaaaag aattttgaag gatccggaat cggcggcagg
     8581 aactttagac cggcgatcgg gcgaagcaac ttctcgcgtt cggcggagcc tgcgagttct
     8641 tccagcgccg cgagcatctg agagccgctg gtgagggcga ctttgaccga cattgccgga
     8701 accgcccgat gcagcgcgtt cgcggccgcg acgacgtcga caacgctttc gccacggaca
     8761 atgccgaaac gcgcttccct gccaagctga ggatcgacaa aagttgcaag acgcatattc
     8821 actttcctcc cggcaattgc acgtcgttgt ctttcacgag cttgctccat t
//
LOCUS       DASWGM010000997         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_804710,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000997 DASWGM010000000
VERSION     DASWGM010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804710"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VGA15_26900"
     CDS             complement(<1..966)
                     /locus_tag="VGA15_26900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9591358.1"
                     /translation="MAELKADVLVLGAGMVGVSAALHLQQRGRDVILVDRRELAGEET
                     SFGNAGMIESASVFPYMFPRDFGQILQYAINGSPQVRYQFFDLPSFLPWLVRYYLASS
                     PDRALHSATAELPLIRRSLIEHEALIAEAEVPELLRRTGWIKLFRSDATLANAVRDFE
                     RAKQYGVAGELLDSNAIAMREPNLTGEFAGAIHFPAPGFVPDPGGLAKAYAALFKRKG
                     GRFLVSDARTLEQVPGGWRVGGRDGTAVGREIVVALGPWSDLVFGPLGYSIPLGVKRG
                     YHLHLAPRGNAVLNHPVLDSDLGYLLAPMNRGIRLTTGVEFARRDR"
     gene            1166..2764
                     /locus_tag="VGA15_26905"
     CDS             1166..2764
                     /locus_tag="VGA15_26905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591359.1"
                     /translation="MSVRQTLLAATTVVIVAFAMSPASAQTLRYANQGTLKSLDPYTL
                     NESTTHAHLGHVYEGLVTRDKDLKTIPALAESWEAPEPTRWRFHLRKNVKFHNGDPFT
                     ADDVVFSAERVQAKGSNLQTRIPAGTKVVKVDDYTVDFVLTSPNPILTGQWDTWYIMD
                     KKWAEANNTVEPTPVAATSPSFASLHANGTGPFTVESHEPGVKTVYKANPNWWRKPEH
                     NLKEIVFTPIASDATRVAALLSGEVDVIEPVPIQDIARVNASAGASVLTGPELRTIFL
                     GMDQVRDELLYSNVKGKNPFKDIRVREAFYKAVDVDLIKNRVMRGLSTPSALMVAPQL
                     FKLSGDFTRPKLDPEGAKKLLTEAGYPDGFEVTMDCPNDRYVNDAAICQAIVGMLARI
                     GVKIDLLAQPKAQYFAKVLKPGGFQTSFYMLGWTPSTLDSQNVLYDIMGCRDDLKSPR
                     GEANLGGYCNKQMDAIADKVLLETDTAKRDLLIKEAFEVAAKDFGYIPLHQQALAWGV
                     SKKVKLTQRADNQVLLYWATKQEE"
     gene            2867..3847
                     /locus_tag="VGA15_26910"
     CDS             2867..3847
                     /locus_tag="VGA15_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9591360.1"
                     /translation="MLAFTLRRAIQAVGVMIAVGIISFSMFRFAGDPVNQIVSLDTSA
                     AERAAISKSLGLDDPVPVQFVRYFGNAMRFNFGMSYQFRQPVANLLMERMPATLELAT
                     CATILAMVFGILMGVYSALKRDTVLAKILQAVSLIGISLPTFLIGILLIYLFAVVLGW
                     LPSFGRGNVVRIGWWTTGLLTVSGLKALILPSITLGLFQMTLIMRLVRAEMLEVLRTD
                     YIRFARARGLTTRAIHFGHALKNTLVPVITVAGLQFGSVIAFAIITETVFQWPGMGLL
                     FVQAVQNVDIPIMAAYLLMVSLIFVTINLVVDILYTVVDPRLRSTISRPA"
BASE COUNT          789 a         1214 c         1137 g          748 t
ORIGIN      
        1 ccgatcgcgg cgggcaaatt cgacgccggt ggtgagacgg atgccgcggt tcatcggcgc
       61 cagcagatag ccgagatccg aatcgagaac gggatgattg agcacggcat tgccgcgcgg
      121 cgccagatgc agatggtatc cgcgcttgac gccaagcggg atggaatatc cgagcggacc
      181 aaataccaga tcggaccagg gtcccaacgc cacgacaatt tcgcgcccga ccgcagtacc
      241 gtcgcgaccg ccgacacgcc atccgccggg aacctgttcg agcgttctcg catcgctcac
      301 gagaaagcgc ccacccttgc gcttgaacag cgccgcatag gctttggcca atccgcctgg
      361 atcgggcacg aagcccggcg caggaaaatg gatggcaccg gcaaactctc cggtcagatt
      421 gggctcgcgc atcgcgatgg cgttgctatc gagcagctcg cctgcgacgc catactgctt
      481 cgcgcgttcg aaatcccgca ccgcgtttgc aagagtcgca tcggagcgaa acagcttgat
      541 ccagccggtc cgccgcagca actccggcac ttcggcttcg gcgatcagcg cctcgtgctc
      601 gatcaggctg cgccggatca ggggtagctc ggccgtcgcg ctgtgcagcg cccgatccgg
      661 cgaggatgcg agatagtagc gcaccagcca cggcagaaag ctcggcaggt cgaagaactg
      721 gtaacgcacc tgcggtgagc cgttgatcgc gtattgcagg atctgtccaa aatcccgcgg
      781 aaacatgtag ggaaagaccg acgcggactc gatcattccg gcgtttccga aactggtctc
      841 ctcgccggcg agttcgcgcc tgtcgaccag gataacgtcc cgtccccgct gctgcagatg
      901 caaggcggcg ctgacgccga ccatgcccgc ccccaatacg agaacatcgg ccttcagttc
      961 cgccatccgg cgctcccgtt gaatgttaag tgtttgctca tgctgcatgc agtgaagcta
     1021 gttattgcgg cattgccgcg caaaccggcc tggcgttaag gaaatcgcgc ttgcgtggcg
     1081 gccgaactct ggggcaacat ggatatgcga tagctgtctt taatcccaaa aatgaacagc
     1141 gcgccacagc ggagaacgtg aacaaatgtc ggtacgtcaa accttgcttg cagcgactac
     1201 tgtcgtgatc gtcgctttcg cgatgtcgcc ggcatcggcc cagacgctgc gctacgccaa
     1261 ccagggtaca ctcaaatcgc tcgatcccta caccctcaac gagagcacca cccacgcgca
     1321 tctcgggcac gtttatgaag gcctggtgac gcgcgacaag gacctgaaga ccattcccgc
     1381 actggcggag agctgggaag cgccggagcc gacccgctgg cgattccacc tgcgcaagaa
     1441 cgtcaaattc cataatggcg atcccttcac cgccgacgac gttgtcttct cggcggagcg
     1501 cgttcaggcc aagggctcga acctgcaaac ccgcattcca gccgggacaa aagtcgtcaa
     1561 ggtcgacgac tacaccgttg atttcgtcct gacctcgcca aatcccattc ttactgggca
     1621 atgggatacc tggtacatca tggacaagaa atgggccgag gcgaacaaca cggtggagcc
     1681 gacgccggtc gctgcgacct cgccgagttt tgcctcgctc cacgccaacg gcaccggtcc
     1741 tttcacggtc gagagccacg agcccggcgt gaaaaccgtc tacaaggcaa atccgaactg
     1801 gtggcgcaag cccgagcaca atctcaagga aattgttttc acgccgatcg cctccgacgc
     1861 cacgcgcgtc gccgccttgc tgtcaggtga ggtcgacgtg atcgagccgg ttccgatcca
     1921 ggatattgcc cgggtcaatg caagcgccgg tgcttcggtc ctgaccgggc cggaactgcg
     1981 caccatcttt ctcggcatgg atcaggtgcg cgacgaactg ctgtattcga acgtcaaggg
     2041 caagaatccg ttcaaggata tccgcgtccg cgaagcgttc tacaaggccg tcgacgtcga
     2101 cctgatcaag aaccgcgtga tgcgcggcct ttcgacgccg tcggcgctga tggtcgcgcc
     2161 acagttgttc aagctctcgg gcgatttcac ccgcccgaaa ttggatcccg aaggcgccaa
     2221 gaaactcctg accgaagccg gctatcccga tggtttcgaa gtcaccatgg attgccccaa
     2281 cgaccgctac gtcaatgacg ccgcgatatg ccaggcgatc gtcggcatgc tggcccgcat
     2341 tggggtcaag atcgaccttt tggcgcaacc caaggcgcag tatttcgcca aggtgctgaa
     2401 gcccggcggt ttccagacct cgttctacat gctgggctgg acgccgagca cgctcgattc
     2461 gcagaacgtt ctctacgaca tcatgggctg ccgcgacgac ctgaaatcgc cgcgcggcga
     2521 ggccaatctg ggcggctatt gcaacaagca gatggacgcg attgccgaca aggtgctgct
     2581 tgagaccgac accgccaaac gcgatttgct gatcaaggag gcgttcgaag tcgcagccaa
     2641 ggacttcgga tatatcccgc tgcatcagca ggccctggca tggggggtgt cgaagaaagt
     2701 gaaattgacg cagcgtgcgg acaatcaggt tctgctgtac tgggcgacca aacaggaaga
     2761 ataggcagct tcaatacaaa ggtcccggtg gcttcgcgcc cccgggactt ttcatttccg
     2821 gcggacagcg acgtccgcca cagcaaaaga acagtgaaag gatcacatgc tcgctttcac
     2881 tcttcgccga gccatacaag ccgtcggcgt catgattgcg gtcgggatca tctcgttctc
     2941 gatgttccgc ttcgccggcg acccggtcaa ccagatcgtc tcgctcgata cctcggctgc
     3001 cgagcgcgcc gcgatcagca agtctctcgg gctcgacgat ccggtgccgg tgcagttcgt
     3061 gcgatatttc ggcaacgcga tgcgattcaa tttcggcatg tcgtaccagt tccgccagcc
     3121 ggtggcgaac ctcctgatgg agcggatgcc ggcaacactg gaactcgcca cctgcgccac
     3181 gattctggcg atggtgttcg gcatcctgat gggagtctat tcggcgctca agcgcgatac
     3241 ggtgcttgcc aagatcctgc aggcggtttc gctgattgga atctcgctcc cgaccttcct
     3301 gatcggcatt ctgctgatct atctgttcgc ggtggtgctg ggctggctgc cgtcgttcgg
     3361 ccggggcaat gtcgtgcgga tcggctggtg gaccaccggg ctattgacgg tctccggcct
     3421 caaggccctg atcctgccgt cgatcacgct cggcctgttc cagatgacat tgatcatgcg
     3481 gctggtgcgg gccgaaatgc tggaggttct gcgaaccgac tacatccgct tcgcgcgggc
     3541 gcgcggattg acaacgcggg cgatccattt cggtcacgcg ctgaaaaata ccctggttcc
     3601 cgtcatcacc gtcgccgggc tgcagtttgg ctcggttatt gcattcgcaa tcattaccga
     3661 aacagtattc caatggccgg gaatgggact gctttttgtg caagcggtgc aaaacgtcga
     3721 tattccgatc atggcggcct acctgctgat ggtgtccctg atcttcgtca ccatcaatct
     3781 cgtggtcgat attctctaca ccgtagtcga tccgcgcctg cgctcgacca tcagccgccc
     3841 ggcctgagcg agagatcttc atgaccgaag tcgccttccc gcacaaag
//
LOCUS       DASWGM010000998         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_531047,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000998 DASWGM010000000
VERSION     DASWGM010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531047"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VGA15_26915"
     CDS             complement(<1..552)
                     /locus_tag="VGA15_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX9591361.1"
                     /translation="MTTLLIAEHEHATLKDSTNKALTAATQLGAEVHVLVAGGGEGTK
                     AAAEAAAKLAGVTKVLLAEGDAYAHDLAEPLAALIVALAPSYDAFVAPATSRFKNVMP
                     RVAALLDVMQVSEIIKVISPDTFERPIYAGNAIQTVKSKDAKKVITVRTSTFAAAGDG
                     GSAAVENAASAADPGLSSFVGEEV"
     gene            complement(553..1302)
                     /locus_tag="VGA15_26920"
     CDS             complement(553..1302)
                     /locus_tag="VGA15_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX9591362.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKSDGSGVELSNVKMSMNPFDEIAVE
                     EALRLREAGKATEVVVVSIGPAQAAETIRTGLAMGADRGILVKAEGNVEPLAVAKILK
                     AIADEEKPGLIILGKQAIDDDSNQTGQMLAALLGWSQATFASKLEVDGSDFKVTREVD
                     GGLQTLKLKGPAIVTTDLRLNEPRYASLPNIMKAKKKPIADKSASDYGVDLTAHLEIL
                     KTSEPPGRKGGVKVKDVAELVSKLKNEAGVL"
     gene            complement(1423..1995)
                     /locus_tag="VGA15_26925"
     CDS             complement(1423..1995)
                     /locus_tag="VGA15_26925"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX9591363.1"
                     /translation="MVVLNRIYTRTGDDGTTALGSGERRPKHDLRIGAYGTVDETNAA
                     IGIVRLHLSDARDLDTMLGLIQNDLFDLGADLAVPQREGKAERLRMLSSQVERLEHDI
                     DTLNARLAPLTSFVLPGGTPAAAYLHLARTICRRAERMMVELAAKPDEPVSDAAIQYI
                     NRLSDFLFVASRSVNDNGAGDVLWVPGQNR"
     gene            complement(2009..>2164)
                     /locus_tag="VGA15_26930"
     CDS             complement(2009..>2164)
                     /locus_tag="VGA15_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX9591364.1"
                     /translation="VGAVALVLLLGLLNMMRGGSPNRSQKLMRLRVLLQFVAIVITML
                     ALLAMGR"
BASE COUNT          383 a          709 c          644 g          428 t
ORIGIN      
        1 aacttcctcg ccgacaaaac tggacagacc aggatcggcc gccgacgcgg cattttccac
       61 cgctgcgctg ccgccgtctc cggccgcggc gaaggtcgag gtgcggacgg taatgacctt
      121 cttggcgtcc ttcgatttca ccgtctggat cgcattgccg gcatagatcg gccgctcgaa
      181 tgtgtcgggc gaaatcacct tgatgatttc cgacacctgc atcacgtcga gcagggcggc
      241 gacccgcggc atgacgttct tgaagcgcga ggtggcgggc gcaacgaagg cgtcatagga
      301 cggcgccagc gccacgatca gggccgccag cggctcggcg aggtcgtggg cataggcatc
      361 gccttcggcg agcagaacct ttgtcacgcc ggcgagtttg gccgcagcct cggccgcggc
      421 cttggtgccc tcgccgcctc cggcgaccag aacatgaacc tcggcgccga gctgggtggc
      481 cgcggtcagc gccttgttgg tggagtcctt cagggtggcg tgttcgtgtt cggcaatcaa
      541 cagcgtggtc atctagagaa ccccggcttc gttcttgagc ttcgacacca gctcggcgac
      601 gtccttgacc ttgacgcccc ccttgcggcc cggcggttcg gatgtcttga ggatctcaag
      661 atgggctgtg agatccacgc cgtaatcgga ggcgctcttg tcggcaatcg gcttcttctt
      721 ggccttcatg atattgggca ggctggcgta gcgcggctcg ttcagccgca gatcagtagt
      781 tacgatcgcc ggtcccttaa gtttcagcgt ctgcaagccg ccgtcgacct cgcgcgtcac
      841 cttgaaatcg gagccgtcga cttcgagctt ggacgcaaag gtcgcctgcg accaccccag
      901 cagcgcagcc agcatctggc cggtctggtt gctgtcatcg tcgatcgcct gcttgccgag
      961 aataatcagg ccgggtttct cttcgtcggc gatcgctttc agaattttcg ccaccgccag
     1021 cggttcgacg ttgccttccg ccttgaccag aataccgcgg tcggcgccca tcgcgagacc
     1081 ggtgcggatg gtttccgccg cctgcgccgg accgatcgat accaccacga cttcggtcgc
     1141 cttgccggcc tctcgcaggc gcaaggcttc ctcgacggcg atttcatcga acggattcat
     1201 cgacatcttg acgttggaca gttcgacgcc cgatccgtcg ctcttgacgc gaaccttgac
     1261 gttgtaatcg accacccgct ttaccggcac cagaaccttc atcgatcctc tttcgcttga
     1321 attcaggttt gatcagcctg cgcgcggaac ctaaagctcc cgccaacccc ggtcaacgcg
     1381 cgaaggccaa aaatcgcccc cgacatacgc acttaacggc gcttagcggt tctggccggg
     1441 cacccacagc acgtcgccag caccgttatc gttcacggaa cggctggcga caaacaaaaa
     1501 atccgacaag cggttgatat actgaatcgc ggcatcgctg accggttcgt cgggctttgc
     1561 cgcgagttcc accatcatcc gttccgccct gcggcatatc gtacgggcca gatgcaggta
     1621 cgccgcggcc ggcgtgcctc cgggcaacac aaacgatgtc aacggcgcca gccgcgcatt
     1681 caacgtgtcg atatcgtgtt caaggcgttc gacctggctc gacagcatcc tcaagcgttc
     1741 cgccttgccc tcgcgctggg gcaccgcgag gtcggcgccg aggtcgaaca gatcgttctg
     1801 gatcagcccc agcatcgtat cgagatcgcg cgcatccgac agatgcagcc gcacaatgcc
     1861 aatggcggcg ttggtctcgt cgacagttcc ataggcgccg atgcgcaggt cgtgtttcgg
     1921 gcggcgctcg ccgctgccaa gtgcggtggt accgtcgtcg ccggttcggg tgtagattcg
     1981 attgaggacg accattatct gtctcccttc agcggcccat cgccaacagc gcgagcatgg
     2041 tgatgacaat cgcgacgaat tgcagcaata cccgcaaccg catcagtttc tgggaccgat
     2101 tgggcgatcc gccccgcatc atgttgagga ggccaagcag cagcaccagc gcgacggcgc
     2161 cgac
//
LOCUS       DASWGM010000999         6164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2216471,
            whole genome shotgun sequence.
ACCESSION   DASWGM010000999 DASWGM010000000
VERSION     DASWGM010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216471"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VGA15_26935"
     CDS             complement(<1..266)
                     /locus_tag="VGA15_26935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591365.1"
                     /translation="MIRTAITLAMLLCATAAQAQTVIEKLCIVTAAQDLPHVPGLAIK
                     ASRITTAPADYLGNQDMKYFGAEIDVVAAGQTMTFAFACGTSPR"
     gene            489..1007
                     /locus_tag="VGA15_26940"
     CDS             489..1007
                     /locus_tag="VGA15_26940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591366.1"
                     /translation="MKLLLAGFISLLAASAAAQPVNKPTNGTELRSAQAEASKPDNVP
                     VGGCMPIGLTARGDLVFPMQCRELIERERGPVPEQQLHVPLQSSRQEPAKATAAEKDV
                     AATGQTPASQVQIGLAPADQTQTGQLQAGEFLMGQTQKRGRKKLSRAERRAKQGLPPE
                     SAPQVTGSITRN"
     gene            complement(1041..1403)
                     /locus_tag="VGA15_26945"
     CDS             complement(1041..1403)
                     /locus_tag="VGA15_26945"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591367.1"
                     /translation="MTRSDAETRWQQRFSRQDQSNDGNEADHSARATALIEPDAPIEG
                     NINAVPAFDKWRSIHTEWLLTGRVTQPDHRLKVEFRLWNVIRGTQLLGQQYFLQPEQW
                     RNVSHVIAEAILKRLNDE"
     gene            complement(1400..2260)
                     /locus_tag="VGA15_26950"
     CDS             complement(1400..2260)
                     /locus_tag="VGA15_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9591368.1"
                     /translation="MPEFRLTQISDTHLARRLPSLTQNFHRLSEYIDATRPDLVINSG
                     DLAFDGPTNRDDLEFARELHHALPVACRYLPGNHDIGDNPTAVGPVPPQLATEQDRQT
                     FLEIVGEDRWRFEAAGWCFIGLNSLVMNTAIASEAEQFDWLASELSSTKGKPVALFLH
                     KPLYLNTPDDPELPATSIRYVPMPARSRLIDMLRAVDLRLVGSGHVHQRRDFTFGHVR
                     HVWAPSAGFIISDKMQEVIGTKEVGLVEYRFQPDSFEVRHIRAPGQIDVNLDSLIGAI
                     SKSQDAPVQT"
     gene            complement(2422..3135)
                     /locus_tag="VGA15_26955"
     CDS             complement(2422..3135)
                     /locus_tag="VGA15_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acriflavin resistance protein"
                     /protein_id="HEX9591369.1"
                     /translation="MNIISPIPEGAAHAAGPPGYAVTSFLKMVVDSSGLFADDSPDGL
                     LGMLMSGLAGVATALAVAIVLTVYFRSGYRSARDIFKHGFAAAAVLALLAFVASDMRH
                     AALAYLGINPSKPAVEFEIRLPKAAASSVANSQIELHTDRNQTLAQVQAALGSDEDGR
                     SIIRGSVPLEFRTTERVVILNLPGQPQCQFKLRLAASPSRSDQFGPWHLADRVASAVG
                     GEETRTEQNDAFAIRYRVL"
     gene            3312..3734
                     /locus_tag="VGA15_26960"
     CDS             3312..3734
                     /locus_tag="VGA15_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX9591370.1"
                     /translation="MSVQMVLLPVLVLVGLAFALLFAMATARGQALSGRETSPKDIAL
                     GQPNWPTHATQIGNCFSNQFELPVLFYILIALALPLRHADFVIVLLSWVFVITRFAHA
                     GIFVTSNNVQQRSQAWLAGVLVLFAMWLYFALKVLLLI"
     gene            3793..5097
                     /locus_tag="VGA15_26965"
     CDS             3793..5097
                     /locus_tag="VGA15_26965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX9591371.1"
                     /translation="MTPAARLSAAIELIDTIDTQRVPAAKALKEWGTAHRYAGSGDRA
                     AISGLIWDVLRRRASSAWIMDNDTARARVLGMLKVERAMDADAIAALCDGGRFAPEPL
                     SGAERAALTSRSLAGAPAPIAGDYPEWLDGYLAQVFGDDRVAEATAMASRAPLDLRVN
                     TLKAKREKILGSMAHLGATPTPWSPIGLRIELGADARNPGIHAEEDFIKGAIEVQDEG
                     SQLAALLSAAKPGEQVIDLCAGAGGKTLALAAMMQGKGRLIATDHDKRQLAPIYERLS
                     RAGVHNCDIRTPKGEGDTLVDIHASADLVLIDAPCTGTGTWRRNPDAKWRMRPGALEV
                     RLKEQVTVLDRAAALTKPGGRIAYITCSVLPPENGEQIRSFLARHPEFSVVPPLQTVT
                     VLWDKAEDFAAATLQSPEGLLMTPRRTGTDGFFVSILKRNTS"
     gene            5132..5836
                     /locus_tag="VGA15_26970"
     CDS             5132..5836
                     /locus_tag="VGA15_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9591372.1"
                     /translation="MKRYHRIDLLGAAAIFLGLAAGSAFADETVNIGGSRAVLIKPDA
                     PRASVILLPGGDGAINAGDHGDIHSLNFNQLVRTKNAYAARGLAVLVADANTPLDAAV
                     QYMAAIKRPVTVIATSRGTIRAAEGIARGARPNALVLTSGFLSAESGSGQNVMSILGS
                     PASLPRTLVIHHRQDSCRSTLPAGVEPFVKWSAGRARAAWVSGGTSEGDPCEARAYHG
                     FNGVDGQVVGLAAGFH"
     gene            5943..>6164
                     /locus_tag="VGA15_26975"
     CDS             5943..>6164
                     /locus_tag="VGA15_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HEX9591373.1"
                     /translation="MIHYAAAHARQIQREPDTFWLSRHRKGPGMQYVNSIFGELLKPI
                     DRRQFREIVERHDGNAYDKTFKSWDHLVTM"
BASE COUNT         1141 a         1829 c         2019 g         1175 t
ORIGIN      
        1 tgccgcggcg aggttccgca ggcaaacgca aaggtcatgg tttggccggc ggccacgacg
       61 tcgatctcgg caccaaaata tttcatgtcc tggttgccga gatagtcggc cggcgccgtc
      121 gtgatgcgac tggctttgat cgcaagaccg ggaacgtggg gcaaatcctg cgccgcggtc
      181 acgatgcaca atttttcgat cacggtttgg gcctgcgccg cggtcgcgca cagtagcatc
      241 gccagcgtga ttgcagttct gatcattgcc gtctccgata gcggtccgcc ggagggatcg
      301 tcgaggcgcc ggcccggcag ggcgttggcg ttcgcttgca tccgtacagc gcgatataat
      361 tacatcacgt gtggactcac gtgatagcgc caggtccaaa tgcttatatt tcgggcattc
      421 gacgattgat atttcgagca gcgccgtggt agtgcagaat ctttagcgta cgcgtaaagg
      481 acgttctgat gaagctcttg ctcgcaggct ttatctcgct gctcgccgct tcggcggcgg
      541 cgcagccggt taacaagccg accaacggaa cggaattgcg cagcgcacaa gcagaggctt
      601 cgaagccgga caatgtgccc gtcggcgggt gcatgccgat cggcctgacc gcccggggtg
      661 accttgtttt tccgatgcag tgccgcgaat tgatcgagcg agagcgtggc cccgttccgg
      721 agcaacaact gcatgtaccc ctgcagagtt ctcgacagga gccggcgaag gcaactgcgg
      781 cagagaagga tgttgctgcg acggggcaga ctccagcgag tcaggtgcag atagggctag
      841 ctccagcaga tcaaacccag acaggtcagc ttcaagctgg tgaatttctg atgggccaaa
      901 ctcaaaaacg cgggcgcaaa aaactttcca gggccgagcg acgcgccaag caagggcttc
      961 cgccggaatc cgctccacag gtgaccggga gcatcacgcg gaattagaac gatccaaacc
     1021 aggtcctgct attggcgtgg ttattcgtcg ttcaaccgtt tgaggatcgc ctccgcgatc
     1081 acgtgcgaaa cgttgcgcca ttgttccggt tggagaaaat actgctgtcc gagcagctgc
     1141 gtgcctctga ttacattcca taggcgaaat tccaccttga ggcggtggtc gggctgcgtg
     1201 acgcggccgg tcagcagcca ttcggtatgg atgctgcgcc atttgtcgaa tgccggcacg
     1261 gcgttgatat ttccttcgat cggcgcgtcg ggctcgatca gtgcagtagc gcgcgccgag
     1321 tgatcggctt cattgccgtc attggattga tcctgtcgcg aaaatcgctg ctgccaacgc
     1381 gtttcagcgt cgctgcgcgt caggtttgca cgggcgcgtc ctggcttttc gaaatcgcgc
     1441 cgatcagcga atccaggttg acgtcgatct ggcccggcgc gcggatgtga cggacctcaa
     1501 aactgtcggg ctggaagcga tactccacga ggccgacttc cttggtgccg atcacttcct
     1561 gcatcttgtc ggaaatgatg aagccggcgg aaggcgccca gacgtgccgg acgtggccaa
     1621 acgtaaaatc ccggcgctgg tgcacatggc cgcttccgac cagccgcaga tcgacggcgc
     1681 gcaacatgtc gatcaggcgg ctgcgcgccg gcatcggaac gtaacgtatc gatgtcgccg
     1741 gaagctccgg atcgtcaggg gtattgaggt aaagtggttt gtgaagaaac agcgctaccg
     1801 gcttgccctt ggtgctggag agttctgacg cgagccagtc gaactgttcg gcctcgctcg
     1861 caattgcggt gttcatgaca agcgaattca ggccgatgaa acaccagccg gcggcctcga
     1921 aacgccagcg gtcttcgccg acaatctcaa gaaaagtctg ccggtcctgc tcagtggcca
     1981 gttgaggcgg aacggggccg acggcggtcg ggttatcgcc gatatcgtga ttgccgggca
     2041 gatagcggca ggcgacggga agcgcatggt gcagctcgcg ggcaaattcg agatcgtcgc
     2101 gattggtcgg cccgtcgaac gcaagatcgc cgctgttgat gacgagatcg gggcgcgtcg
     2161 cgtcgatgta ttcgctcagc cgatgaaagt tttgcgtcaa actcggcagg cggcgggcaa
     2221 gatgggtgtc ggaaatctgg gtcaggcgaa attcgggcat gcaacatttc ctcccggccc
     2281 aattatacct tttggcggaa gtcgaaacga tgacaattta ccagcgtcgc ctcacacagc
     2341 cacggccgtc atcatccgcc gcccggtcgg cgcaacgcgc cgcccggtgg cggggcatga
     2401 cgactagtcg tttgttcacg ttcacagcac gcgatagcgg atcgcgaagg cgtcattctg
     2461 ctcggtgcgg gtttcttcgc ccccgaccgc cgaagcgacg cgatcggcca gatgccatgg
     2521 cccgaactga tcggagcggc ttgggcttgc ggcgagacgc agcttgaact ggcattgcgg
     2581 ctgacccggc aggttcagga tcaccacccg ttcggtggtg cggaattcga gcggcaccga
     2641 acccctgatg atgctgcggc cgtcttcatc ggatcctagc gcggcttgca cctgggccag
     2701 cgtctggttg cggtcggtgt gcagttcgat ctggctgttg gccacggagg acgcagcggc
     2761 cttgggcagg cggatttcga actcgaccgc gggttttgac gggttgatgc caagataggc
     2821 cagcgcggca tgccgcatgt cggaggccac gaaggccaga agcgcgagca ccgccgcggc
     2881 ggcgaagccg tgtttgaaga tatcgcgcgc gctgcgatag ccgctgcgga aatagacggt
     2941 caggacgatg gccaccgcca gcgcggtcgc gacgcccgcc agccccgaca tcagcatacc
     3001 gagcaatccg tccgggctgt cgtcggcgaa aagaccgctg ctgtcgacga ccattttgag
     3061 gaacgaggtc acggcgtaac cgggcggccc ggccgcatgg gccgcgccct ctgggatcgg
     3121 ggaaataatg ttcatcgcag cctctcgctg gtcgcgtttg gtgcctaatc gagactgaaa
     3181 tcacttgatc taatgaaagt tagtcacggg aatgcgcatg gtggttcaac ccgtccgcgc
     3241 aggatttatt gtcaggggca agtgctgccg ctatagcggg ggcttagcac ctggtcccgc
     3301 ggagaattcc gatgtccgtt caaatggttt tgctgccggt cttggtcctg gtggggctcg
     3361 cttttgcgct tttgttcgcc atggccacgg cgcgtggcca ggcgctctcg ggccgggaaa
     3421 ccagccccaa ggacatcgcg ctgggtcagc cgaactggcc cacccacgcc acccagatcg
     3481 gcaattgctt ttccaaccag ttcgaactgc cggtcttgtt ctacatcctg atcgcgctgg
     3541 cgctgccgct gcgccatgcc gacttcgtaa tcgtgctgct gtcgtgggtg ttcgtgatta
     3601 ctcgttttgc ccatgccgga attttcgtga cgtcgaacaa cgtccagcag cggtcgcagg
     3661 cgtggctcgc cggcgtcttg gtgctgtttg cgatgtggct ctatttcgcg ctcaaggtat
     3721 tgctgctgat ctgagccaag tgccctcgaa gtgtccccga actggcaccg gtgctcgaac
     3781 tgaaggtttt gaatgactcc cgcagcgcgg ctctccgcgg ccatcgaact gatcgacacc
     3841 atcgacacgc aacgcgtccc ggcggcgaag gcgctgaagg agtggggcac cgcgcatcgt
     3901 tatgccggct cgggcgatcg cgccgcgatc tccggcctga tctgggacgt gctgcggcgg
     3961 cgggcgtcaa gcgcctggat catggacaac gacacagcgc gcgcgcgcgt gctgggcatg
     4021 ctgaaggtcg agcgcgccat ggatgccgat gccatcgccg cgctctgcga cggcggccgg
     4081 ttcgcgccgg agccgttgag cggggccgag cgcgccgcgc tgacgtcgcg gtcgcttgcc
     4141 ggtgcgccgg cgcccatcgc cggcgattat ccggaatggc tcgatggata tctggcgcag
     4201 gtatttggcg atgaccgcgt cgcggaggcg accgcgatgg cgagccgggc gccgctcgac
     4261 cttcgcgtca acaccttgaa ggcgaagcgc gagaagattc tcggctcgat ggcgcatctc
     4321 ggcgcaacgc cgacgccgtg gtcgccgatc ggcctgcgca tcgagctcgg cgccgacgcc
     4381 cgcaatcccg gcatccatgc cgaggaggat tttatcaagg gcgccattga agtgcaggac
     4441 gagggctcgc agctggcggc tttgctgtcg gccgcaaaac ccggcgagca ggtgatcgac
     4501 ctctgcgccg gcgccggcgg caagaccctg gcgctggcgg cgatgatgca gggcaagggc
     4561 cggctgatcg ccaccgatca cgacaagcgg cagctcgcgc cgatctatga gcggctgtcg
     4621 cgcgccggcg tccacaattg cgatatccgc accccgaagg gcgagggcga tacgcttgtc
     4681 gacatccatg cttccgctga cctcgtgctg atcgatgcgc cctgcacggg cacaggcacc
     4741 tggcggcgca atccggatgc caaatggcgg atgcgcccgg gagcgctcga ggtgcggctg
     4801 aaggagcagg tgacggtgct ggatcgcgcc gcggcattga caaaaccggg tgggcggatc
     4861 gcctatatca cctgctcggt actgccgccg gaaaacggcg agcagatccg cagctttctc
     4921 gcgcgccacc cagaattttc cgtggtgccg ccgttgcaga cggtgacggt gctgtgggac
     4981 aaagcggagg atttcgccgc cgcgacgctg caatcccccg aggggctatt gatgacgccg
     5041 cgccgcaccg ggacggatgg gttttttgtc tcaatactga aaaggaatac atcgtaattt
     5101 tagttgagta cggatttgaa cggagaacat catgaagcga taccatcgga tcgatttgtt
     5161 gggcgccgcc gcaatatttc ttggccttgc cgctggttcg gcctttgccg atgagaccgt
     5221 caacatcggc ggctcccgcg cggtattgat aaagccggac gcgccgcggg cgagcgtgat
     5281 cctgctgccg ggcggcgacg gggcaatcaa tgcgggcgac cacggggata tccactcgct
     5341 taacttcaac cagctggtcc gcacaaaaaa cgcctatgcg gcgcgagggc tggcggtgct
     5401 ggtggccgat gcgaataccc ccctcgacgc cgcggttcaa tacatggctg cgatcaagcg
     5461 tcctgtcacc gtcatcgcca ccagtcgcgg cacgatccgc gccgcggagg ggatcgcgcg
     5521 cggcgcccgg ccgaacgcgc tggtgctgac ttcaggattt ctcagcgccg agtcgggcag
     5581 cggtcaaaat gtcatgtcga tcctcggctc gccggcgtcg ttgccgcgca cgctcgtcat
     5641 ccatcacagg caggatagct gcaggtcgac cttgccggcc ggtgtcgagc cgttcgtcaa
     5701 atggtcagcc ggccgcgcgc gggcggcctg ggtgagcggc ggaacaagcg aaggcgatcc
     5761 ctgcgaggcc cgggcctatc acggcttcaa tggcgtcgac ggtcaggtcg tggggctggc
     5821 ggcgggattc cattgatgtc cgggcgacag cgcaatcttc taatgcgaga tgagtttaga
     5881 ttgagcggca gcaacaacgg aagtgcgccc cctctcccgc ttgcggcagg gctgtcccgg
     5941 gaatgattca ctatgccgca gcgcatgccc ggcaaatcca gcgtgaaccg gatactttct
     6001 ggttgtcgag acacagaaaa ggaccgggca tgcaatacgt gaatagcatc tttggggagc
     6061 tcctcaaacc gattgatcga cggcaatttc gggaaatcgt cgagcgccat gacggcaatg
     6121 cgtacgacaa aacgttcaaa agttgggatc atctcgtgac gatg
//
LOCUS       DASWGM010001000         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1490441,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001000 DASWGM010000000
VERSION     DASWGM010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490441"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            499..1452
                     /locus_tag="VGA15_26980"
     CDS             499..1452
                     /locus_tag="VGA15_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX9591374.1"
                     /translation="MPRHVNYVPHGVIPAVLLPFDNDLAIDEASFRKHLRDVASVEDL
                     SAITVNAHSTEVGSCSFEEQRRVLDIAQDEIGAKLPLINGIWADGSLEAARLARMATQ
                     GGASALLVFPPAPFTLGQSPQMALAHFKRIADATDLPLIVFQYPLATGQGYPKDTLLK
                     LCEEVPTVRAIKDWIGNVPHHEWHLRTLQNLPRRVNVLTTHSAWLFSSLVLGCNGLLS
                     GSGSVIADLQARLFRAVQGNDLAEAKRLNDRIEPLMRVFYADPFVDMHNRMKEALVLL
                     GKLPRAVVRPPLVKIERSEIMRIRQGLIEAGLLDKTARDAA"
     gene            complement(1566..2360)
                     /locus_tag="VGA15_26985"
     CDS             complement(1566..2360)
                     /locus_tag="VGA15_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEX9591375.1"
                     /translation="MLPAAGMQRGSRYGGGHRGPTAGLAPGFVQGNLAILPAPLASDF
                     LRFCQFNPKPCPLIGTSATGDPRVPELGEDLDIRFDLPRYGMWRNCDLVAESEDVRDL
                     WRDDLVSFVIGCWFSFEEALMAEGIELRHIARGRNVTMYRTSIAAHAAGPFHGPMVVS
                     MRPLRPADAIRAVQITTRFPAVHGAPVHIGKPELIGIADLMKPDYGDAVPVNDDELPV
                     FWACGVTPQSLIATVRPEFCITGYPGSMLVNGPKEFRNSRLCDPSA"
     gene            2784..>3512
                     /locus_tag="VGA15_26990"
     CDS             2784..>3512
                     /locus_tag="VGA15_26990"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591376.1"
                     /translation="MTEKWKDEEWAQDIWYRGKVTRRRIVGWGGGAIGAMMLVPAPWR
                     AAFGAAKPYKIGSEQPLSGAGAAGGKTALVGLQMAVDRINHMGGIMGRPIELVIADDE
                     SKPDVARRKTQKMVEEDNIDVHVGGFLSNICLACMPVWEEAKIVNMISVCLDTTLTTS
                     KCNRHSFRVHDYAPAQAVAFGPMLAKMGKKWHIAYADYSWGQSTRDAYADEIKKNGGE
                     VVGTTGIPLGTADMTPFLSKISGNF"
BASE COUNT          647 a         1120 c         1129 g          616 t
ORIGIN      
        1 gcgcgatatg cgcgcttgga ctgagactcc agctctgcct cattcgagcg agtaagcaat
       61 ctggccgaaa atcgtcgcca agtgcagatt gcttggaaac tcatgatgtc ggtcggaagc
      121 tcgccagtct aagaacagcg atcgtgaaag cggcttgatg cgcaaatcaa cttacggctt
      181 cggcttgctg ctgatttaat gccaatcatt gcggctcgac gtttggagca atgcccgacg
      241 ccggcagcca ctgcgaggca gagacgctgc agaagcgcgc gcctcgtgcg cgccgctgcc
      301 gaccggctat cggcggcatt cggctacgcg agcgagcccg agccgggggg tgaaggcggc
      361 ggtgcgattt tgccgtcatt ccgggacggc acgtagcgcc ggacccgaaa tccagataca
      421 aattcggtgc ctgtttctgg attccgggtt cgctcgttct gctcgcgccc cggaatgacg
      481 gggaatcgga ggcgagccat gccccggcat gtgaactatg tcccgcacgg cgtgatcccg
      541 gcggtgctgc tgccatttga taacgatctc gccatcgacg aggctagctt ccgcaagcac
      601 cttcgagacg tggcctccgt cgaagacctc tcggccatca ccgtcaacgc gcactcgacc
      661 gaggtcggct cctgcagttt cgaggagcag cgccgcgtgc tcgacatcgc gcaggacgag
      721 atcggggcca agctgccgct gatcaacggc atctgggccg acggcagcct cgaggcggcg
      781 cgccttgcgc gcatggcgac gcaaggcggc gcgtccgcgc tcttggtgtt cccgccggcg
      841 ccgttcaccc ttggccaatc gccgcaaatg gcgctcgcgc atttcaagcg catcgccgac
      901 gcgaccgacc tgccgctaat cgtgttccag tatccgctcg cgaccggcca aggctatccg
      961 aaggacacgc tgctgaagct gtgcgaggag gtgccgaccg tccgcgccat caaggactgg
     1021 atcggtaacg tgccgcatca cgagtggcac ctgcgcacgc tgcagaatct gccgcggcgg
     1081 gtgaatgtgc tcaccacgca cagcgcctgg ctgttctcct cgctggtgct cggctgcaat
     1141 ggattgctgt caggttccgg cagcgtgatt gccgatttgc aggcgcgcct gttccgcgcg
     1201 gtgcagggca acgacctcgc cgaggccaag cggctcaacg accgcatcga gccgctgatg
     1261 cgggtgttct acgccgatcc cttcgtcgac atgcacaacc gcatgaaaga agcgctggtg
     1321 ctgctcggca aattgccgcg cgcagtggtg cgcccgccgt tggtgaagat cgagcgctcg
     1381 gagatcatgc gcattcgtca ggggctgatc gaggccggtt tgctcgacaa gaccgcgcgc
     1441 gatgcggcgt aacctctctc cgtcgttctg aggtgcgagc gaagcgagcc ttgaaagatg
     1501 aaccgcccgg gccgtcgccc ttcgagggcc gcgctgcgcg cggccgcctc aggctgactg
     1561 tcgcctcagg ctgacggatc acataatcgc gaatttcgga attctttcgg tccgttcacc
     1621 agcatgctgc cggggtaacc cgtgatgcag aactccggcc tcaccgtcgc aatcaacgat
     1681 tgcggcgtca cgccgcaggc ccaaaatacc ggcagctcgt cgtcgttgac cggcaccgcg
     1741 tcgccgtaat ctggcttcat caggtcggca atgccgatca gttccggctt gccgatgtgc
     1801 actggcgcgc cgtgcacagc gggaaagcgc gtggtgatct gcaccgcgcg gatcgcatcg
     1861 gccggtctca gcggccgcat cgataccacc atcggcccat ggaacgggcc tgccgcgtgg
     1921 gcagcgatcg aggtgcggta catcgtcacg ttgcggccgc gcgcgatgtg acgcagttcg
     1981 atgccctccg ccatcagcgc ttcttcgaat gaaaaccagc agccgatgac gaaactcacc
     2041 agatcgtcgc gccagaggtc gcgcacgtcc tccgattcgg ccaccaggtc gcagttccgc
     2101 cacatgccat agcgcggcag atcgaagcga atgtcgaggt cctcgccgag ttccggcacg
     2161 cgcggatcgc ccgtggccga ggtgccgatc agcggacaag gcttcggatt gaactggcag
     2221 aaccgcaaaa aatcgctcgc cagcggggca ggaaggatgg cgagattacc ctgcacaaag
     2281 ccgggcgcga gccccgcggt cggcccgcga tggccgcccc cgtatcgcga gccgcgctgc
     2341 atgcccgctg ccggcagcat cgtcgcggcg aagggcggtg acgttcatgg gcgggctccg
     2401 atccgggcaa atcgcgcgcg gcaagtcgag catccgccgc cggttgcgcg agcggcgctt
     2461 gctcatttcg cgagcaacat attcgaccaa gtgagtacgt ctatgggttg aaacttgccc
     2521 tagatcgcgt ttgctaaaga gagcagatta ctcagcaaga gcccgccaag ggccgcaaga
     2581 gggaggatgg tcgatgctgg ctcatgccgc ctgcgtgcat ggagttccaa ggtccgcagc
     2641 tgtgctttgc attgcacggc acgtgcggat gacgacatcg actgaccgac tgcacacttg
     2701 gctcttccgc attgccgccc gcacaatagc gcgggcgtaa ttttatcgac cgacctgatc
     2761 ggaacgcatg ggaggaacgg attatgaccg agaagtggaa agacgaagaa tgggcgcagg
     2821 acatctggta tcgcggcaag gtcacgcgtc gtcgcatcgt cggttggggc ggcggggcga
     2881 tcggcgcgat gatgctggtg ccggcgccct ggcgcgccgc cttcggcgcc gccaagccgt
     2941 acaagatcgg ctccgagcag cccttgtccg gcgccggcgc cgccggcggc aagaccgcgc
     3001 tggtcggcct gcagatggcg gtcgaccgca tcaaccacat gggcggcatc atgggccggc
     3061 cgatcgagtt ggtgattgcg gacgacgagt ccaagcccga cgttgcccgc cgcaagacgc
     3121 agaagatggt ggaagaggac aatatcgacg tccatgtcgg cggcttcctt tccaatatct
     3181 gtcttgcttg catgccggtc tgggaagagg ccaagatcgt caacatgatt agcgtgtgcc
     3241 tcgacaccac gctcaccacc tccaagtgta accgccacag cttccgtgtc cacgactacg
     3301 ctccggcgca ggcggtcgcc ttcggcccga tgctcgccaa gatgggcaag aagtggcaca
     3361 tcgcttatgc tgactattcg tggggccagt cgacgcgcga cgcttacgcg gacgagatca
     3421 agaagaacgg cggcgaggtc gtcggcacga ccggcatccc gctcggtacc gccgacatga
     3481 cgccgttcct gtcgaagatt tccggcaact tc
//
LOCUS       DASWGM010001001         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2738195,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001001 DASWGM010000000
VERSION     DASWGM010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2738195"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VGA15_26995"
     CDS             <1..141
                     /locus_tag="VGA15_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEX9591377.1"
                     /translation="LAGINLPMLVKLAKVREERSLADAIAMAQEAGRKYVTIASRVLA
                     GK"
     gene            138..467
                     /locus_tag="VGA15_27000"
     CDS             138..467
                     /locus_tag="VGA15_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX9591378.1"
                     /translation="MSDDDALPEIDRGPSVPSGAVSREIPIINKRGLHARASAKFVQM
                     VERFSAEVWVTKGNETVGGTSIMGLMMLSAGPGTSIVVSAIGPEAQAAVDALTELVAS
                     KFNEESA"
     gene            complement(562..2187)
                     /locus_tag="VGA15_27005"
     CDS             complement(562..2187)
                     /locus_tag="VGA15_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX9591379.1"
                     /translation="MSTASFLPAASRARGRLSIRRMAAWLAACASDPKAAPRLVIGFA
                     AAHAVLWTLILVNLKAAQDVHMDVAEAFAWGQKFQFGYGKHPPLAGWVAGVWFRIFPV
                     ANWATYALAMATLGCGLVICWLIALRVVDRRRAFFVVVMLALYPIFNFKGFKYNPDLL
                     QLVTLPLLVLAFLHAFEQRSWRSGLWLGLAGALALMTKYWVLTMIGAIGIAALIHPDR
                     WLFLRSPAPWVAIATLVVAMVPHLIWLKEVDFVPLTYAGDVYGLSSRAQSIQLVLGYV
                     GHNLALLAAPVVLAALALAWSPRWWMTRARQPLALFTRAWSRGENRCVNVSQALNIWI
                     IQIVVAVGPPLGGLIFTVYMKTDWGISLFFLTPLALVAIPSLRLHRMALFHIAAIWLV
                     ITLAVLAASPTIAARELAENPNGASGYGARSELARELTEAWQTRFHTRWAVVAGTTEI
                     GEPMTFYSPDHPAPFTPGEVWSSGLTSLEEAKRLGFIGICDTSDPRLPVCDAWMAANA
                     DHAERLAITTQRFFHGHPGPAIGWKVYLAPPAQ"
     gene            2302..>3173
                     /locus_tag="VGA15_27010"
     CDS             2302..>3173
                     /locus_tag="VGA15_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX9591380.1"
                     /translation="MTSIPISNIRNFAIVAHIDHGKSTLADRLIQTTGGLQAREMKEQ
                     VLDSMDIERERGITIKAQTVRLTYPAKDGKTYILNLIDTPGHVDFAYEVSRSLAACEG
                     SLLVVDASQGVEAQTLANVYHALDAGHEIVPVLNKIDLPAAEPDQVKRQIEDVIGIDA
                     SDAVMISAKTGLGIDDVLEAIVHRLPAPKGDRGASLKALLVDSWYDVYLGVVVLIRVV
                     DGVMKKGQRIRMMGTNAAFDVERVGFFTPKMTQIDELGPGEIGFITAAIKEVADTRVG
                     DTITDDRKPVTDMLP"
BASE COUNT          653 a         1025 c          999 g          496 t
ORIGIN      
        1 ctcgcaggca tcaatcttcc catgctggtc aagcttgcca aggtgcgcga ggagcggtcg
       61 ctagccgacg ccatcgccat ggcccaggaa gccggccgca aatacgttac catcgccagc
      121 cgggttctcg ccggcaaatg agcgacgacg acgcgttgcc cgaaattgac cgcgggccaa
      181 gtgtcccgtc aggcgcggta tcccgggaaa ttcccatcat caacaaacgc ggtctgcacg
      241 cccgcgcctc cgcgaaattc gtccagatgg tcgagcgctt cagcgccgaa gtctgggtga
      301 caaaaggcaa cgagacggtc ggcggcacct ccattatggg gctgatgatg ctgtccgcgg
      361 ggccggggac atcgatcgtc gtctccgcga tcggccccga agcgcaagcg gccgtcgatg
      421 cgctcaccga actggtcgcc agcaagttca acgaagaaag cgcgtgattg gcgcgaagtt
      481 cggagcgtta gactagcttc gcaaccgcgg aagcgcgctc cctcgccccg ctgtcttgcg
      541 gggagaggtc gacagaacac gctattgcgc cggcggcgcg agatagacct tccagccgat
      601 cgccggaccg ggatggccgt ggaagaagcg ctgcgtggtg atggcgagac gctcggcgtg
      661 atcggcattc gccgccatcc aggcgtcgca gaccggcagc cgtggatcag acgtatcgca
      721 gatgccgata aacccgagcc gtttggcctc ctcgagcgac gtcagcccgg acgaccagac
      781 ctcgccgggc gtgaacggcg ccggatgatc cggactgtag aacgtcatcg gctcgccgat
      841 ctccgtggtg ccggcgacca ccgcccatcg cgtgtggaaa cgggtctgcc aggcctcggt
      901 cagttcgcgg gccagttcgg agcgcgcgcc atagcctgag gcgccattgg gattctcggc
      961 gagctcgcgg gcagcgatcg tgggcgacgc ggcgagcacc gcaagcgtga tcacgagcca
     1021 gatcgcggca atatgaaaca gcgccatcct gtgaaggcgc agcgacggaa tcgcgaccag
     1081 cgccagcggc gtcaagaaga acagcgagat accccaatcg gttttcatgt agaccgtgaa
     1141 aatcaggccg ccgagcggcg gtccgaccgc gaccacgatc tggatgatcc agatattgag
     1201 cgcctgcgat acattcacgc accggttctc cccgcgcgac catgcgcgcg tgaacagcgc
     1261 caatggttgc cgcgcccgcg tcatccacca tcgcggtgac catgccagcg caagtgctgc
     1321 cagcaccacc ggtgccgcga gcagcgccag attgtggccg acatagccga ggacgagttg
     1381 aatgctctgc gcgcggctcg acaggccgta gacgtcgccg gcataggtca gcggcacgaa
     1441 atcgacttct ttcagccaga tcagatgtgg aaccatggca acgacgagcg tcgcgatcgc
     1501 gacccacggc gccggcgagc gcaggaacag ccaccggtcg ggatggatca gtgcagctat
     1561 cccgatggca ccgatcatgg tcagcaccca gtactttgtc atgagcgcca gcgcgccggc
     1621 gaggccaagc cacaggcctg atcgccacga gcgctgctcg aatgcgtgca gaaaagccag
     1681 caccagcagc ggcagcgtta ccaactgcag cagatccgga ttatatttga agcccttgaa
     1741 attgaagatc ggatagagcg cgagcatcac cacgacgaag aaggcgcggc ggcgatcgac
     1801 cacgcgtaac gcgatcagcc agcaaatcac gaggccacag ccgagtgttg ccatcgccag
     1861 cgcgtaagtg gcccagttgg cgaccggaaa aatcctgaac cagacacccg cgacccatcc
     1921 cgccagcggc ggatgcttgc catagccgaa ctggaatttc tggccccagg cgaaggcttc
     1981 cgcgacgtcc atgtgaacgt cctgcgccgc tttgagattg accaggatca gggtccacag
     2041 cacggcatgg gccgcggcaa aaccgattac cagccgaggg gcggctttcg gatcgctggc
     2101 gcaagccgca agccacgccg ccatccgccg gatcgataac cggccgcgcg cgcgcgaggc
     2161 cgcgggtaag aacgatgcag tagacatggc ctcgtgcgta gcgcgttttt gcggtcagtg
     2221 gtatcagctt ggcgtgaaga agccccgctg aaacgccgca ttatggttgc cgccccgggg
     2281 tgcgaatgct atcccgcgcc catgacatcc atccctattt ccaacatccg caacttcgcc
     2341 attgttgcgc atatcgacca tggcaaatcg acgctggccg accgtctgat ccagaccacc
     2401 ggcggcttgc aggcgcgcga gatgaaggaa caggtgctcg attcgatgga tatcgagcgc
     2461 gagcgcggca tcaccatcaa ggcgcagacc gtgcggctga cctatccggc caaggacggc
     2521 aagacctaca tcctcaatct gatcgacacg cccggccatg tcgattttgc ctacgaggtt
     2581 tcccgctcgc tggcagcctg cgaaggttcg ctgctggtgg tcgacgccag ccagggcgtc
     2641 gaggcgcaga cgctcgccaa cgtctaccat gcgctcgatg ccggccacga gatcgtaccg
     2701 gtgctcaaca agatcgacct gccggcggcg gaaccggatc aggtcaaacg tcagatcgag
     2761 gacgtgatcg gcatcgacgc ctcagacgcg gtgatgatct cggccaagac cggcctcggc
     2821 atcgacgacg tgctggaagc catcgtgcac cggctgccgg cgccgaaagg cgaccgtggc
     2881 gcgtcgctga aggcgctctt ggtcgactcc tggtacgacg tctatctcgg cgtcgtggtg
     2941 ctcattcgtg tcgtcgacgg cgtgatgaaa aagggccagc gcatccggat gatgggcacc
     3001 aacgccgcct ttgatgtcga gcgcgtcggc ttcttcacgc cgaagatgac gcagatcgat
     3061 gaactcggcc ccggcgagat cggcttcatc accgccgcga tcaaggaagt ggccgacacc
     3121 cgcgtcggcg acaccatcac cgatgatcgc aagccggtca ccgacatgct gcc
//
LOCUS       DASWGM010001002         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_406743,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001002 DASWGM010000000
VERSION     DASWGM010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406743"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="VGA15_27015"
     CDS             <1..1855
                     /locus_tag="VGA15_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX9591381.1"
                     /translation="TTIAFALLWTFPCLQCGPSMVGPHALHVIAGFVLAALLVVCGLM
                     FGPPADEGRIEPISSGSLAAYLAGSTMIVLASFHTDAAIIVFALLVAGTIFVAWRAQA
                     ATGAVGAAAVFVFVVFAEWAVRANPDMLVLPGGPLPGIGPAATDGSVSLHLVTAAIFA
                     IGFGAAGFLAQDRFTSAIIPVVWSAAAVFTPLTLLIALYARIAHLDRSIPFAILAVVL
                     AAAYGAATEVLTKRHNRPGLPVSIALFATGALGALALALTFALEKGWLTIALALMSMG
                     TAWISMQRPIPFLRSLAAILAGIVVLRIGYEPRIVGDAVGSTPIFNWLLWGYGIPALS
                     FWAGSHFLRRRGDDAPLRTVESAAILFTVLLAFMEIRHAVNGGDVYHDNAGLTEIALQ
                     VCVALAMAIGLERLRIRTGSIVHNVSAILLTVFAGLASIFGLLLLENPMIWWSIDVGG
                     VFINQILLGYALPAVLALLLSYAAAGRRPAFYANSIAAGALVLALTYVTFEIRRLYHG
                     PVLTSGATSGAEQYTYSIAWLAFGVALLGIGIVFNSQRARLASAVVIALTILKAFLID
                     MSTLTGIYRALSFMCLGLVLVAIGWLYQRILFRRQAAPPSDGRHGSSQPLM"
     gene            1923..2498
                     /locus_tag="VGA15_27020"
     CDS             1923..2498
                     /locus_tag="VGA15_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX9591382.1"
                     /translation="MARPRSFDPDEALDLARDVFWQKGFQGTSLDDITAATGLAKPSL
                     YAAFGDKNALFLKVLDRYHERIVGNAERVLKEGPSARDAIERWLTGFVPFCSGAKGSR
                     GCLSVNAAADGASDQKQVRDRIQRFNRKLEELLRNRLRADRAQFSEAFDPEGSAHTLM
                     AIYVGLMVLAKDAPDAARVRATLNQAMKLLA"
BASE COUNT          397 a          815 c          823 g          544 t
ORIGIN      
        1 cacaacaata gcgttcgcgc tgctgtggac cttcccctgc ctgcaatgcg gcccgtcgat
       61 ggtcggaccg cacgcgttgc atgtgatcgc agggttcgtg ctcgccgcat tgctggtggt
      121 gtgcggactg atgttcggcc cgcccgccga cgaaggccgg atcgagccga tttcgtccgg
      181 ctcgctcgcg gcctaccttg cgggctcaac gatgatcgtg ctcgcgagtt tccataccga
      241 cgccgccatc atcgtgttcg ctctgctggt ggccggcaca atattcgtgg catggcgcgc
      301 gcaggccgcc accggcgcgg tcggcgccgc ggccgttttc gtgttcgtcg tgtttgccga
      361 atgggcggtt cgcgccaatc cggacatgct ggtgctgccg ggcgggccgc tgcccggcat
      421 cggcccagcc gccaccgacg gttcggtctc gttgcacctg gtgaccgcgg cgattttcgc
      481 gatcggattc ggcgccgcgg gatttttggc gcaggaccgc ttcacgagcg cgatcatacc
      541 ggtagtatgg tcggcggcgg ccgtgttcac gccgctgacg ctgttgatcg cgttgtacgc
      601 ccgcatcgcc catctcgatc gctcgatccc gtttgctatc ctggcggtgg tgctggcggc
      661 agcgtatggc gccgcgaccg aagttctgac caagcggcac aatcggccgg gattgccggt
      721 ttcgatcgcg ctgttcgcga caggggcgct gggcgcgctg gcgctggcgc tgacctttgc
      781 actcgaaaag ggctggctca cgattgcgct ggcgctgatg tcgatgggca cggcgtggat
      841 atcgatgcaa cggcctatac catttctgcg ctcgctggcc gcgatcctcg ccggcatcgt
      901 ggtgctgcgc atcggctacg agccgcgcat cgtcggcgat gcggtcggta gcaccccgat
      961 cttcaactgg ctgttgtggg gctacggcat tccggcgctg tcgttctggg ccggaagcca
     1021 cttcctgcgc cgccgtggcg acgacgctcc gctgcggacc gtggagtcgg cagcgatcct
     1081 gttcacggtg ctgctggcgt tcatggaaat tcgtcacgcc gtcaacggcg gcgacgtcta
     1141 ccacgacaac gccggcctga ccgagatcgc gctgcaggta tgtgtggcgc tcgccatggc
     1201 gatcggcctg gaacggctgc gcattcgcac cggcagcatt gtccacaacg tcagcgcgat
     1261 cctcctcacg gtgtttgccg gactggcgag catatttggg ttgttgttac tcgagaatcc
     1321 gatgatctgg tggtcgatcg acgtcggcgg cgtattcatc aatcagatct tgctcggcta
     1381 cgccctgccg gcggtgcttg cgctgctgtt gtcttacgcg gccgccggcc ggcgtcccgc
     1441 cttctatgcc aacagcatcg cggcgggcgc gctcgtgctc gcgctgacct atgtaacgtt
     1501 cgaaattcgc cggctctatc acggcccggt gctgaccagc ggcgctacca gcggcgccga
     1561 gcaatacacc tattcgatcg cgtggctggc gttcggcgtg gccctgctcg gcatcggcat
     1621 cgttttcaat tcgcagcgcg caaggctcgc ctccgcggtc gtgatcgcac ttaccattct
     1681 gaaggcgttc ctgatcgaca tgtcgaccct gacgggcatc tatcgcgcgc tatcgttcat
     1741 gtgtctcggc ctggtgctgg tggcgatcgg ctggctgtac cagcggatcc tgttccgccg
     1801 gcaggcggcg ccgccatcgg acgggcgcca cggttcaagc cagcccttga tgtaaatcga
     1861 cggcggtcgc cgccctcgaa acctctcttt gcgatttcgc agtcagcgcg tacataggtc
     1921 cgatggcaag accgcgcagt ttcgatcccg acgaggcgct ggacctcgcg cgcgacgtat
     1981 tctggcaaaa aggattccag ggtacatcgc tcgacgacat caccgccgcc accggccttg
     2041 ccaagcccag cctctatgcg gccttcggcg acaagaatgc gttgttcctg aaagttctgg
     2101 accgctatca cgaacgtatt gtcgggaacg ccgagcgcgt cctcaaggaa gggccgtcgg
     2161 cgcgtgacgc gatcgaacgc tggttgaccg gctttgtccc tttctgctcg ggcgctaagg
     2221 gaagcagggg ttgtctctcg gtcaatgccg cagccgacgg cgcttccgat cagaagcagg
     2281 ttcgcgacag gatccaacgc ttcaaccgga aactggagga attgctgcgc aaccgccttc
     2341 gcgctgaccg tgcgcaattt tccgaggcgt tcgatccgga gggtagcgca catacgctga
     2401 tggcgatcta tgtgggactg atggtgctgg cgaaagatgc gccggatgcg gcccgcgtcc
     2461 gcgccacctt aaatcaggcg atgaagctac ttgcttgaca ctcaatcttt cattatgtac
     2521 tttatagtac ataaccagga gaacacggtc atgcagacga tccagattga agcgttcgg
//
LOCUS       DASWGM010001003         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_406801,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001003 DASWGM010000000
VERSION     DASWGM010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406801"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..517)
                     /locus_tag="VGA15_27025"
     CDS             complement(71..517)
                     /locus_tag="VGA15_27025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591383.1"
                     /translation="MSVTGLSGEICAMKGSDWSFGGSWDGILNLMLLRKELSATDSAY
                     YIIIFGGGCFIVSMLFLCLNLLRQYVVGEPFLKLRHKGFIEHVKVFFAWPLASAITGI
                     VGVWLDILKTSIEACVAVGFAWHIVLIVFFGERTREDKDVGAGEAE"
     gene            complement(647..2080)
                     /gene="tldD"
                     /locus_tag="VGA15_27030"
     CDS             complement(647..2080)
                     /gene="tldD"
                     /locus_tag="VGA15_27030"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX9591384.1"
                     /translation="MTDLARTDALFFAQTGMDRGRVEKIVEQTLDGMDDGELFLEYSQ
                     SESLAFDDGRIKAASFDTAQGFGLRALSGEAAGYAHASELSEAAIRRAADTVRAVKTG
                     HSGSFTAPPIGTNQTLYTDANPLSLIEFGAKAKLLAEIDAYARAKDPRVRQVTASLSG
                     SWQAVQIIRPDGTRVGDIRPLVRLNVSVVVGEDDRMETGSSGAGGRVEYQRYFEPAQW
                     RAQVDEALRQALVNLGSVSAPAGEMAVVLGPGWPGVMLHEAVGHGLEGDFNRKKTSAF
                     AGLIGQRVASPGVTVVDDGTLKDRRGSLTVDDEGTPSSRTVLIEDGMLRGYMQDRLNA
                     HLMGMRPTGNGRRQSYAHAPMPRMTNTYMLAGDKDPKEIIASVKRGLYAVNFGGGQVD
                     ITSGKFVFSASEAYLIENGKVGLAVKGATLIGNGPDALTKISMIGNDLSLDDGIGTCG
                     KNGQGVPVGVGQPTLRMDGLTVGGTAA"
     gene            2339..>3840
                     /locus_tag="VGA15_27035"
     CDS             2339..>3840
                     /locus_tag="VGA15_27035"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX9591385.1"
                     /translation="MKTRTFEARKWQRASTVGASVIALLSLAVAATGHAADSAKPVET
                     ASPIKHLIVIIGENRSFDHVFATYQPKHGQSVANLLSKRIIRADGSPGPNFAQAGQFS
                     VLPRASYYISADRSAKTPFTFLPPPDLNGVPLAASDANIFPPFGTIAKAAAAEPDLEP
                     EDLVLLTTGAATGLSFTPETGGIRNPDTRIANVTRLPNGPFQLTGPTMPYDAYTEDTI
                     HRFYQMWQQTDCATDQEHATRANPTGCLSDLWPFITTTFAGTAENGMGTPMAFLNVAQ
                     GDAPVLKRLADQYTLSDNFHQAAMGGTGVSHIYLGTADNIFFSDGKGRAISPSAAFGP
                     MLGPLLIANPNPVPATNNKYLNDGFYTNCSDLTQPGAGPILDYLNALSYAPSAACDDG
                     HFYLLNNLFPGFHANGAPNTGNARFPILAIVPPSSVRTIGDALIEKNISWAYYGGAFN
                     EAVAAGVDDDLTPFCPICNPFQYAASIMADDAVRKEHTKDILDLFSAIDS"
BASE COUNT          707 a         1319 c         1131 g          683 t
ORIGIN      
        1 tggagccagc ttcttacccc gctctgtgag taaaagcgcc gatccatgcc gagtactttc
       61 tttattaggc tcattcggct tctccagcac ccacgtcctt atcctccctg gtgcgttcgc
      121 caaagaatac gatcagtaca atgtgccagg caaatccaac cgcgacacat gcctctatgc
      181 tagttttaag gatgtcgagc catacaccga ctattcccgt gatggcggac gccaacggcc
      241 aagcgaaaaa cactttcaca tgttcaataa accccttgtg gcgcaacttc agaaatggtt
      301 cgccgaccac atactggcgc aacaaattca ggcacaaaaa gagcatcgag actatgaaac
      361 agccgccgcc gaagataatg atgtagtacg cagagtcggt tgcagacagc tccttgcgca
      421 atagcatcag gtttaggatc ccatcccagc ttcccccaaa ggaccagtcg gatcctttca
      481 tagcgcagat ttcgccgctc agtccggtaa ccgacatgtt ctaccccaga agccgacatg
      541 ttctacccca gaagaatgtc tggacggccg ccgtcagcgt gatgaagtaa ttactatcag
      601 gaagcccccc gcgatttaag acctactgat caatgtcggc cggcgatcat gccgcggtgc
      661 cgccgaccgt gagcccgtcc atgcgcaggg tcggctggcc gacgccgacc ggcacgccct
      721 ggccgttctt gccgcaggtg ccgatgccgt cgtcgaggct cagatcgttg ccgatcatgc
      781 tgatcttggt gagcgcatcg gggccgttgc cgatgagcgt cgcgcctttg accgcgagcc
      841 cgaccttgcc gttctcgatg agataggcct ccgacgccga gaagacgaac ttgccggagg
      901 tgatgtcgac ctggccgccg ccgaaattga cggcgtagag gccgcgcttc accgaggcaa
      961 tgatctcctt ggggtccttg tcgcccgcaa gcatgtaggt gttggtcatg cgcggcatcg
     1021 gcgcatgggc atagctctgg cgccggccgt tgccggtggg gcgcatgccc atgagatgcg
     1081 cgttgagccg atcctgcatg tagcctcgca gcatgccatc ctcgatcagc accgtgcgcg
     1141 aggagggggt gccttcatca tcgacggtga gcgagccgcg gcggtcctta agcgtgccgt
     1201 catcgacgac ggtgacgccc ggcgaggcga cgcgctggcc gatcaggccg gcgaaggccg
     1261 aggtcttctt gcggttgaag tcgccttcga ggccgtggcc caccgcctcg tgcagcatga
     1321 cccccggcca gcccgggccg agcacgaccg ccatttcgcc cgccggcgcc gacaccgagc
     1381 ccagattgac gagcgcctgg cgcagcgcct cgtcaacctg cgcccgccat tgcgcgggct
     1441 cgaaatagcg ctggtactcg acacgcccgc cggcgcccga ggatcccgtc tccatgcggt
     1501 cgtcctcgcc gacgaccacg ctgacattga ggcgcaccag cgggcggatg tcgccgacgc
     1561 gggtgccgtc cgggcggatg atctgcaccg cctgccacga gcccgagagc gacgccgtga
     1621 cctggcgcac gcgcgggtcc ttggcccggg cataggcgtc gatctcggcg agcagcttcg
     1681 ccttggcgcc gaattcgatc agcgacagcg gattggcatc ggtatagagc gtctggttgg
     1741 tgccgatagg cggcgcggtg aagctgccgc tgtgccccgt cttgactgct cgcaccgtat
     1801 cggcggcgcg gcggatcgcc gcttccgaga gctcggaggc atgcgcgtag ccggctgcct
     1861 cgcccgagag tgcgcgcagg ccgaagccct gcgccgtgtc gaaggacgcc gccttgatgc
     1921 ggccatcgtc gaaggccagg ctttccgact ggctgtattc gaggaacagc tcgccgtcat
     1981 ccatcccgtc cagggtttgc tcgacgatct tctcgacgcg accgcggtcc atccccgtct
     2041 gggcgaagaa cagcgcatcg gtgcgtgcga ggtcggtcat tggatactcc cgttggcggg
     2101 tatgcgattg ttgcggcgcg gcatcggtct tgctgcggct tgtgccagaa aggcgctatc
     2161 catgccagcg ggttaaccgg gtgcgcggcg gcgattgcca cacataggcg ctcgcgccgc
     2221 ggccatcaag ccgaaccgcc acgccgcctc tgtcgcgatg ccgaaacgtt ggtgaaatgt
     2281 cttcgtcatc gcgcttctct agcttaccct cctgtcaatt ctaccgggag gcgagccaat
     2341 gaaaacgaga acattcgagg caaggaaatg gcagcgggca tcgacggtcg gtgcctccgt
     2401 catcgcgctg ctgagtttgg cagttgccgc caccggccat gccgccgatt cggccaagcc
     2461 tgtcgagaca gcgagcccga tcaagcacct gatcgtcatc atcggcgaga accgcagctt
     2521 tgaccatgtc ttcgccacct accagcccaa gcacggccag agcgtggcca acctgctgtc
     2581 gaagcggatc atcagggcgg acggaagccc gggtccgaat ttcgcacaag cgggccagtt
     2641 ctcggtcttg ccgcgggcga gctactacat cagcgccgac cgcagcgcca agaccccctt
     2701 caccttcttg ccgccgccgg atctcaacgg cgtcccgctt gcggccagcg atgccaacat
     2761 cttcccgccc tttggcacca tcgccaaggc ggccgctgcg gagcccgatc tcgagccgga
     2821 ggaccttgtc ctcttgacga ccggcgccgc caccggcctg agcttcacgc ccgagacggg
     2881 cgggatccgc aatcccgata cgcgcatcgc caacgttacg cgcctgccca acggcccgtt
     2941 ccagctgacc gggccgacca tgccctacga tgcctacacc gaagacacca tccaccgctt
     3001 ctatcagatg tggcagcaga ccgactgcgc caccgatcag gagcatgcca cgcgcgccaa
     3061 tcccaccggc tgcctcagcg atctctggcc cttcatcacc accaccttcg ccggcaccgc
     3121 ggagaatggc atgggtacgc cgatggcgtt cctcaacgtc gcgcagggcg atgcgcccgt
     3181 gctgaagcgc cttgccgacc agtacacgct gagcgacaat ttccatcagg cagcgatggg
     3241 cggaaccggc gtcagccaca tctatctcgg gaccgccgac aacatcttct tcagcgatgg
     3301 caagggccgc gcgatcagcc caagcgccgc cttcgggccg atgctggggc cgctgctgat
     3361 cgccaacccc aacccggtcc ccgcgaccaa caacaagtat ctcaacgacg gcttctacac
     3421 gaattgctcc gacctgaccc agcccggggc cgggccgatc ctggattacc tcaatgcgct
     3481 gtcctacgct cccagcgcgg cttgcgatga cgggcatttc tatcttctga acaacctgtt
     3541 ccccggcttt catgccaacg gcgcgccgaa caccggcaac gcccgcttcc ccattctcgc
     3601 catcgtgccg ccctcgtcgg tccggaccat cggtgacgcg ctgatcgaga agaacatctc
     3661 ctgggcctat tacggcggcg ccttcaacga ggccgtggcg gccggcgtcg acgatgacct
     3721 cacgcccttt tgcccgatct gcaacccgtt ccagtacgca gcctcgatca tggccgacga
     3781 tgcagtgcgc aaggagcaca ccaaggacat tctcgacctg ttcagcgcaa tcgacagcga
//
LOCUS       DASWGM010001004         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1926633,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001004 DASWGM010000000
VERSION     DASWGM010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1926633"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VGA15_27040"
     CDS             complement(<1..657)
                     /locus_tag="VGA15_27040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591386.1"
                     /translation="MLELKGLSKHFGGVRAVDGLDLRVTQGEVFGLIGPNGSGKSTVV
                     NLVCGLFPVTAGQVLLRNEDITSLASHVRVACGVTRTFQNIRLFGQLSVWQNLWVAQN
                     AVEHRSEGFLRRWLGGPGRAREEIDRILEFSDLSGKRDELAGNLAFGEQRRLELARAV
                     VAKPALLLLDEPAAGMNAEEIDQLDARIRKLKQDGLTVLLIEHHMELVMAVTDRIAVL
                     N"
     gene            complement(632..1561)
                     /locus_tag="VGA15_27045"
     CDS             complement(632..1561)
                     /locus_tag="VGA15_27045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591387.1"
                     /translation="MDFYLGLAQIVGVHTLLGLSAYCVLLTGQVSLAQAGFFAIGAYV
                     AAMLTVLAGWHILPAIAAASFVAGVVACAVGFPALRVKGLMLVVATLAFGEAVRLFFF
                     NFDYQVARGEVKIGPAGGEGFRQIRFFPEHGWSTLDVLLFIWLFVALVMAALWWMDRS
                     RAGAVLRAVGEDEVAAQSAGINVTAVKVAAMTVGGLIAGLGGGIFAHYTAHIEHAQFG
                     VALATFAIAYPILGGLSSVFGTLIAVIFVQGFLIEGLRFLGDWRNLLFGGLILLAMNV
                     RPRGLIDAATIAGIKRLRIRNREPGNARTQGAE"
     gene            complement(1571..>2469)
                     /locus_tag="VGA15_27050"
     CDS             complement(1571..>2469)
                     /locus_tag="VGA15_27050"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591388.1"
                     /translation="QGMSYLDVIDTLQSIADGVLFGATYSLLGIGFTLVFGVMHKINL
                     SYAAASIGAAYASLIVLSVVKAPAIFIFLLAGLSGGIIGGVIYIVCFRFIPQANPLAT
                     LMSTVGALLLIDEIIVHATVGIPVPYPALFSDVIVNAGPFTLRGDLMFMFALGCLSML
                     LLLGLLYRTKLGIATRAVSQQPAAAQLCGISIPRVNGLTFVITGVVGGIAGAMIGAAI
                     GVLSPLLSLPLTTKGLIVTVIGGLGSIPGAIIAGLLVGGFENLFQYFRGVTERDLYVM
                     LLLFAFLVFRPGGIFSPAAGRD"
BASE COUNT          555 a          802 c          765 g          347 t
ORIGIN      
        1 gttgagaact gcaatgcgat ccgtcaccgc catcaccagc tccatgtgat gctcgatcag
       61 cagcaccgtg agtccatcct gcttcagctt gcggatgcgc gcgtcgagct ggtcaatctc
      121 ctcggcgttc attcccgcgg ccggctcgtc gagcagcagc agggcaggct ttgcaacgac
      181 agcgcgggcg agctcgaggc ggcgctgttc gccgaacgcg agattacccg cgagttcgtc
      241 gcgcttgccc gacagatcgg aaaactcgag gatccggtca atttcctcgc gcgcgcggcc
      301 cggaccgccg agccagcggc gcagaaagcc ttcgctgcgg tgttcgaccg cgttctgcgc
      361 cacccacaaa ttctgccaga cgctgagttg gccgaacaac ctgatgttct gaaatgtgcg
      421 ggtaaccccg cacgccaccc gcacatgcga ggcaagggag gtgatgtcct cgttgcgcaa
      481 caggacctgg ccggccgtga ccggaaacag gccgcagacg agattgacga ccgtcgactt
      541 gccggagccg ttcggcccga tcaggccaaa cacctcgccc tgcgtaaccc tcaaatcgag
      601 gccgtcgacc gcgcggacgc cgccgaaatg tttactcagc cccttgagtt cgagcattgc
      661 ccggctccct gttgcgaatt cgcaagcgtt tgatgccggc gatcgtcgcc gcatcgataa
      721 gtccgcgcgg cctgacattc atggccagca gaatgaggcc cccgaacaac aggttgcgcc
      781 agtcgccgag gaagcgcagc ccctcgatca aaaaaccctg gacgaagatg acggcgatca
      841 aggtcccgaa cacgctcgag agcccgccga ggatcggata ggcgatcgcg aaggtggcaa
      901 gcgcgacgcc gaactgggcg tgctcgatat gggcagtgta gtgggcgaag atgccacccc
      961 caagaccggc gatgaggccg ccgacggtca tcgcggccac cttgaccgcg gtcacattga
     1021 tcccggcgct ttgggctgcg acttcatcct cacccacggc gcgcagcacg gcgccggcgc
     1081 gcgagcggtc catccaccat aatgccgcca tcaccagcgc gacaaacagc cagatgaaca
     1141 agagaacgtc gagcgtcgac cagccgtgct ccgggaaaaa cctgatctgc cgaaaacctt
     1201 cgccgccagc cgggccaatc ttcacttcgc cccttgcaac ctgataatcg aagttgaaga
     1261 agaacagccg caccgcctcg ccgaacgcca gcgtcgcgac caccagcatc aggcctttga
     1321 cgcgcagcgc ggggaagccg accgcgcagg cgactacgcc ggcaacgaag gaagctgcgg
     1381 caatcgcagg gagaatatgc cagccggcga gcaccgtcag catcgccgcg acataggcgc
     1441 cgatcgcgaa gaagccggcc tgcgccaggc tgacctgtcc ggtcagcaga acgcaatagg
     1501 cggaaagccc cagcaatgtg tggacaccga cgatctgggc gagcccgaga taaaaatcca
     1561 tgcggtctcc tcaatcccgc ccagcggcgg gggagaaaat gccgcccggc cggaatacca
     1621 gaaacgcaaa cagcagcagc atgacataga ggtcgcgctc ggtgacgccg cggaaatact
     1681 gaaacaggtt ctcgaaaccc ccgaccaaga gaccggcgat gatcgcgccc ggtatagagc
     1741 ccaatccgcc gatcacggtg acgatcagcc ccttggtggt cagcggcagc gacaatagcg
     1801 gcgacagcac gccgatggcg gccccgatca tggcgccggc gatgccgccg accacgcctg
     1861 tgatcacgaa ggtcagaccg ttgacgcgcg ggatcgaaat tccgcacagc tgcgccgcgg
     1921 cgggctgctg cgagaccgcg cgcgtcgcga tgccgagctt ggtgcggtag agcaacccca
     1981 gcagcagcag catcgacagg cagccgagag cgaacatgaa catcagatcg ccgcgcaggg
     2041 tgaaaggtcc ggcattgacg atcacgtcgg aaaacagcgc gggatagggc accgggatac
     2101 ccaccgtcgc atgcacgatg atctcgtcga tcaggagcag cgcgccgacc gttgacatca
     2161 gcgtggcgag tggattggcc tggggaatga agcggaagca gacgatatag atcacaccgc
     2221 cgatgatgcc gccggagagg cccgccagca gaaatatgaa aatcgccggt gccttgacga
     2281 ccgacagcac aatcaggctg gcgtacgccg cgccgatcga cgccgcggcg taggacaggt
     2341 tgatcttgtg catcacgccg aacaccagcg taaagccgat cccgagcagg gaataggtcg
     2401 cgccgaacag aacgccatcg gcaatcgatt gcagcgtgtc aatgacgtcg agataggaca
     2461 tgccttggg
//
LOCUS       DASWGM010001005         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1227405,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001005 DASWGM010000000
VERSION     DASWGM010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227405"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VGA15_27055"
     CDS             complement(<1..785)
                     /locus_tag="VGA15_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX9591389.1"
                     /translation="MNIAFIVDGAAVEAEGGAVFDRKDPMTGKVATRAAAATVADVDK
                     VVEAAAKAFESWSETGPSARRALLLKAADLMESRTADFVKLMLEETGATAPWAGFNVH
                     LAAGILREAAALTTQITGEVIPSDKPGILSMAIRQPAGVVLGIAPWNAPVILGTRAIA
                     AALACGNTVILRSSEICPGTHHLIGQVLNDAGFPKGVVNVLSNAPADAAKIVEALIAH
                     PAVRRVNFTGSTKVGRIIATLAAKHLKPVLLELGGKAPLIVLD"
     gene            complement(823..1518)
                     /locus_tag="VGA15_27060"
     CDS             complement(823..1518)
                     /locus_tag="VGA15_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591390.1"
                     /translation="MMRVRDLHAFYGKSHIIQGLDLDVGPGEIVSVLGRNGVGRSTML
                     KAIMGDVDVQGSVEFAGREMVGLQPYEIARRGLGYVPENREVFAGLTVRENLMMGERS
                     GNAGALWTMEQMFDLFPRLRERAEAPAGVLSGGEQQMLTICRTLMGNPRLIMIDEPTE
                     GLAPMMVELVGRLIRKISESGTAVLLVEQKLAIALQISRRLYVMGHGKIVFEGSPQDI
                     EANRAVRQEWLEV"
     gene            complement(1563..2345)
                     /locus_tag="VGA15_27065"
     CDS             complement(1563..2345)
                     /locus_tag="VGA15_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591391.1"
                     /translation="MAEISSTAALRLDDLHKSFGRTDIIRGVTLEVKQGERHAIIGPN
                     GAGKSTIFNLVSGRMAPTRGRILLDEKRIDGLSPFEVSRLGLSRSFQITNVFPKLSVF
                     ENIRCAILCAEGQAFSLWRNLNGVRAINERCGHVLESIGLADRRETLAESLSYAEMRA
                     LELGLTIAGDAHVILLDEPMAGMSRTEGERALELIRNISAGRTLLLVEHDMAVVFEIA
                     DWISVLVYGEIVATGTPAEIRANAAVQEAYLGQAAHHNGARP"
     gene            complement(2338..3573)
                     /locus_tag="VGA15_27070"
     CDS             complement(2338..3573)
                     /locus_tag="VGA15_27070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591392.1"
                     /translation="MNARFALTWASAAVLLIVLPLIFRSDSAIAVMCIIGVSVVFALS
                     YNMLLGQAGLLSFGHAIFFGLGGFCAIHVMNAVAASHAPVPLVLIPLVGGLGGLFFGI
                     VFGSLASKRGGTIFAMISFGLGELVSSAAPILNRFFGGESGITTDRSALYAPFGINFG
                     PQIQVYYLIAGWCFISSLAMYFVTRTPFGQIMRAVRENPERLEFVGCSTRQVRFVAFV
                     LSAFFAGVAGALAAINFEIMTVSNVGGNQSAMVVLMTYIGGIGYFVGPVIGAVLITLM
                     QILLSDITAGWQMYLGILFVVVVMYAPGGIAALFGPEGIAAQAWHSPRQFLLVIPLLL
                     TAVGGMLLIELGYQVGLKSSDGPIRVLFGIAVNSRSPLPWLVGLVLVIGGFCGLRLVT
                     APADRMPAVLRERTKVRHG"
     gene            complement(3636..4556)
                     /locus_tag="VGA15_27075"
     CDS             complement(3636..4556)
                     /locus_tag="VGA15_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591393.1"
                     /translation="MLNGLLYGMLLFLLASGLTLIFSMMGVLNIAHASFYMLGAYFGF
                     QIAKEIGFWPALLIAPLAVGLIGALIERYGLRRVHAYGHVAELLFTFGLAFIIEELVQ
                     MIWGRVPADYRIPAGLNTTAFTLFNTSYPTFRVFILLVAVAVFALLMLLVTKTRVGLI
                     VRAALTQPRMVGMLGHDVPRIFMMVFGLGSALAGLAGVIAGPVFVTQPSMAGVFGPIL
                     FVVIVVGGLGSLAGALIASLLIGLVQTFAVALNVSIGTVMAAVGIVSPPLLLDELWPV
                     TIAQIAPVVPYLLMVLLLIFRPTGLLGRRM"
BASE COUNT          990 a         1486 c         1341 g          752 t
ORIGIN      
        1 tcgtcgagca cgatcagcgg cgccttgccg ccgagttcaa gcagcaccgg cttcaagtgc
       61 ttggcggcga gggttgcgat gatcctcccc accttagtgg agccggtgaa attgacccgg
      121 cgaaccgccg gatgcgcgat cagcgcctcg acgattttcg ccgcatcggc cggcgcattc
      181 gacaggacat tcacgacgcc tttcgggaat ccggcgtcgt tgagcacctg gccaatcaga
      241 tgatgggttc ccggacagat ttccgacgag cgcaggatca ccgtattgcc gcaggccagc
      301 gccgccgcga tggcgcgggt accgagaatc accggggcgt tccacggcgc aatgccaagc
      361 acgacacctg cgggctggcg gatcgccatc gacaggatgc ccggcttgtc cgatggaatc
      421 acttcaccgg tgatttgcgt cgtcaaggct gcggcctccc ggagaattcc ggcagcaagg
      481 tggacattga agccggccca cggcgcggtg gcaccggtct cctcgagcat caatttgacg
      541 aaatcggcgg tcctgctttc catcaggtcg gccgccttca gcaagagcgc gcgccgcgcc
      601 gacgggccgg tttccgacca gctttcgaaa gccttcgccg cggcctccac caccttgtcg
      661 acatcggcga cggttgccgc cgcggcccgc gtcgcgacct tgccggtcat cgggtccttg
      721 cgatcgaata ccgctccccc ctcggcttcc accgccgcgc catcgacaat gaatgcgata
      781 ttcatgttgg ttcctcttat cggttgctcg ttcatcgttg cgtcagacct ccagccattc
      841 ctgcctcacg gcccggttgg cttcgatatc ctgcggggat ccttcgaaga cgatctttcc
      901 atgacccatg acatagagac ggcggctgat ttgcagcgca attgccaact tctgctcgac
      961 caacaacacc gcggttccgc tctcggatat cttccggatc aggcgtccaa cgagctcgac
     1021 catcatcggg gccagcccct cggtcggctc atcgatcatg atcaggcgtg gattgcccat
     1081 cagcgtgcga caaatcgtca gcatctgctg ctcgccgccc gacaaaaccc cggcgggagc
     1141 ttctgcccgc tcgcgaagcc tcgggaacag gtcgaacatc tgctccatcg tccacagcgc
     1201 gccagcgttg ccgctgcgct cacccatcat cagattctcg cgcaccgtca gtcccgcaaa
     1261 gacctcgcga ttctcaggga catagccgag ccctctgcgg gcgatctcgt atggctgaag
     1321 tccgaccatc tcccggcccg caaattcgac cgatccctgg acatcgacat cgcccatgat
     1381 ggctttcaac atggtcgagc ggcccacgcc attgcggccg agcacgctga cgatttctcc
     1441 cgggccgacg tcgagatcca gtccttggat gatgtggctc tttccgtaaa acgcatgcag
     1501 gtcgcgcaca cgcatcagcg ccgacgcttc gcgcgcgcac gacggttttg caggcgcatt
     1561 cgtcatggac gcgccccatt gtggtgcgcg gcctggccca aatacgcctc ctgcacggcg
     1621 gcgttggcgc ggatttccgc cggcgttccg gtcgcgacga tctcgccgta gaccaatacg
     1681 gaaatccagt ccgcgatctc aaacaccacg gccatgtcgt gctcgaccag aagtagcgtg
     1741 cggcccgccg aaatgttcct gatcagctcg agcgcccgct ccccctcggt tcgactcatc
     1801 ccggccatgg gctcatcgag caggatgaca tgcgcgtcgc ccgcgatcgt caaaccaagt
     1861 tcgagcgcgc gcatttccgc atagctgagg gattccgcca aggtctcgcg tcgatcggcc
     1921 agacctatgc tttcgagcac atgtccgcag cgctcattga tcgcgcgcac gccattcagg
     1981 ttacgccaca gcgaaaatgc ctgcccctcg gcgcacagga tggcgcagcg gatgttctcg
     2041 aatacgctca gcttggggaa tacgttggtg atctggaaac tgcgcgacag gccgagacgg
     2101 ctcacttcga acggcgaaag gccatcgata cgcttttcgt ccagaaggat acggcctcgc
     2161 gtcggcgcca tgcgtccgct gacgagattg aaaatggtgg acttgcctgc gccgttcgga
     2221 cctatgatgg cgtgccgctc gccctgcttc acttccaggg tcacgccgcg aatgatgtcg
     2281 gtccggccga aacttttgtg cagatcgtcc aatcgcaatg cagcagtgga tgatatttca
     2341 gccatgccga accttcgtcc gttcgcgcag caccgcaggc attctgtcgg ccggtgcggt
     2401 tacgagccgg agcccgcaaa agccgccgat gaccagcact aatcccacga gccacggcaa
     2461 cggcgaccgc gaattgacgg cgatgccgaa cagcacgcga atcggcccgt ccgaactctt
     2521 caacccgacc tgatatccga gttcgatcag caacattccg ccgacggccg tcagcaggag
     2581 cggtatgacc agaaggaact ggcgcggcga atgccaggcc tgcgcggcaa tgccttcggg
     2641 gccgaacagc gccgcgatgc cgccgggcgc gtacatcaca accacaacaa acaggatgcc
     2701 gagatacatt tgccatcctg ccgtgatgtc ggagagaaga atctgcatca gggtgatgag
     2761 aacagcgccg atcacggggc cgacgaaata tccgatgccg ccgatatagg tcatcaggac
     2821 gaccatcgcc gactgattgc ccccgacatt gctcactgtc atgatctcga aattgatggc
     2881 cgcgagagcg ccggcgacgc cggcaaaaaa agcggaaaga acgaaggcaa cgaaacgcac
     2941 ctgtcgcgtg ctgcagccga cgaattcaag ccgctccgga ttttcgcgca ccgcacgcat
     3001 gatctggccg aacggcgtgc gggtgacgaa atacatggcg agcgacgaga tgaagcacca
     3061 tcccgcgatc aggtaataga cctggatctg aggaccgaaa ttgattccga agggcgcgta
     3121 gagcgccgag cgatcggtcg tgataccgct ctcgccgccg aaaaaacggt tgaggatcgg
     3181 cgccgctgac gacaccagct cgcccagacc gaacgagatc atcgcgaaga tcgtgccgcc
     3241 gcgcttgctg gcaagcgagc caaagacaat tccgaaaaac aacccgccca gcccgccgac
     3301 cagcgggatc agcacaagcg gaaccggtgc gtgagatgct gcaacggcgt tcatcacgtg
     3361 aatggcacag aaaccgccga gaccgaagaa gatcgcgtgt ccgaacgaaa gcagaccggc
     3421 ctgtccgagc agcatgttat aggacaacgc aaacaccacg gagacaccga tgatgcacat
     3481 cacggcgatc gcggaatcgg acctgaatat caacggtagt acaatcaaga gcaccgcggc
     3541 cgaggcccat gtgagcgcga accgcgcgtt cattgcgagc gagttaaccg accgagccgt
     3601 tccaagactc ccggcagaca attgggaatg ggccgtcaca tccgcctccc gagcagaccg
     3661 gtcggacgga agatcaacag cagcaccatc agaagatagg gcacgaccgg cgcaatctgc
     3721 gcgatcgtga ccggccacaa ttcgtccagc agcagcggcg gcgagacgat cccgaccgcc
     3781 gccatgacag tgccgatcga aacgttgagc gccaccgcga aggtctggac caggccgatc
     3841 agcagcgagg cgatcagggc gccggcaagt gagcccagcc cgccgaccac gatgacgacg
     3901 aaaaggatcg ggccgaacac gccggccatc gagggctgcg tcacgaacac cggccccgcg
     3961 atcaccccgg cgaggccggc aagcgcgctg cccagtccga acaccatcat gaagatgcgc
     4021 ggcacatcat ggcccagcat gccgaccatc cgcggttggg tcagcgccgc gcgcacaatc
     4081 aggccaacgc gggtcttggt caccagcagc atcagcagcg cgaagaccgc gaccgcgacc
     4141 agcaggatga agacccggaa agttggatag ctggtattga acagcgtgaa cgctgtcgtg
     4201 ttcagtccgg ccgggatgcg atagtcggcc ggcacgcggc cccagatcat ctggaccagt
     4261 tcctcgatga tgaacgcgag gccgaaggtg aagagaagct cggcgacatg accataggcg
     4321 tggaccctgc gcagcccgta gcgctcgatc agcgctccaa tcaagcctac ggcaagggga
     4381 gcaatcagca aggccggcca gaatccaatt tccttggcga tctggaaacc gaaataggcg
     4441 ccaagcatgt aaaagctggc gtgcgcgata ttcagcacac ccatcatgct gaatatcagc
     4501 gtgaggccgc tggcgagcag aaacagcagc atgccgtaaa gcagtccgtt cagaattgaa
     4561 aatgtgacg
//
LOCUS       DASWGM010001006         4180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1630805,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001006 DASWGM010000000
VERSION     DASWGM010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630805"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VGA15_27080"
     CDS             <1..495
                     /locus_tag="VGA15_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591394.1"
                     /translation="GAPSLLAAKALGGEMDATVNYWNFCAALEAKGFRRLAGIEDLLP
                     KLGAKGRTAMIGYVFDEEWGSANRDAVTRFIAMTREAKEILATSDAEWEKIAPLTGAA
                     DAATLRAYRDRYREGIPRRPIADEEADARVLYRVLAAIGGREIVGRAQELAPGTFYRA
                     IPGD"
     gene            497..1234
                     /locus_tag="VGA15_27085"
     CDS             497..1234
                     /locus_tag="VGA15_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9591395.1"
                     /translation="MLRLLSFTVFLATWWGAALLAGGEKLPPPPAVLAAIVAEANSGA
                     LFFNLGVTLARVALAFTLAMTLGAAIGYVMGRIRLADRLGDPWLILLLNLPALVVIVL
                     AYIWAGLTEVAAIAAVAINKLPIAVVMVREGTRVLDTALDEMTAVFAIPRWRAFRHVI
                     LPQLAPYIAAAARSGLSLVWKIVLVAELLGRPNGVGFEIGVAFQLFDIPRLLAYALTF
                     AAVVLAIETVLVQPFETRLSRWRPRAA"
     gene            1224..1952
                     /locus_tag="VGA15_27090"
     CDS             1224..1952
                     /locus_tag="VGA15_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9591396.1"
                     /translation="MQLEVDIARKAFTNAADERHDVLADFNFALGAGEVAVFVGPSGC
                     GKTTMLKIVAGLDHDYQGRVACPASARVGMVFQEPRLLPWRSVDDNVRLAAPKASDEK
                     LSALFAVLELTAHRNHFPGELSLGLARRVALARAFAIEPDFLILDEPLASLDDALSAR
                     LRDQIATLVDSRSVTTLLVTHDMDDAIRLGDRVFLLSPRPARILAELPIRTPRNARGE
                     AEIAAIKAEIARRTNGDCAKGDVL"
     gene            1999..2793
                     /locus_tag="VGA15_27095"
     CDS             1999..2793
                     /locus_tag="VGA15_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX9591397.1"
                     /translation="MKRAFGGAALSLSLLAVTAMAQDMMRGVDLSSPDMVSAEMTRGE
                     VEAALIMASGAAPADFTGKRLSSLDLSGLDLSGAIFRAARLNKTKLAGAKLDRAILDQ
                     AWLLQADLTGASLKGANLFASQMLRARLDGADLSQARVAADLTGASLVGASIAGANLG
                     ADMRNQSMGLMRAVLKSANLERLNARGADLSRVDLEFASLKGADLTGASLKSAQLGGA
                     DLTGVTVIGTDFDGADLASAKLIAPIGLDQAKNFDKAKNRDRLIRQ"
     gene            2829..3173
                     /locus_tag="VGA15_27100"
     CDS             2829..3173
                     /locus_tag="VGA15_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591398.1"
                     /translation="MRWILASVVLLLASAGEPAAQGKGIRFWNLTTATISSFQLSPAG
                     KDTWGPNQTLNDKDKEVDHDERLRITGVEPGRYDARISYRDKRKCLVRDIEIKADSVF
                     SITDKDLMDCSK"
     gene            complement(3210..3446)
                     /locus_tag="VGA15_27105"
     CDS             complement(3210..3446)
                     /locus_tag="VGA15_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591399.1"
                     /translation="MNPIDLIVTVCAVLSPATCEETHLMFSGAVSLQQCAMGAPPYVA
                     QWVGEHPKWNAVRWRCEYPHSNDKADARGATPAG"
     gene            complement(3576..>4180)
                     /locus_tag="VGA15_27110"
     CDS             complement(3576..>4180)
                     /locus_tag="VGA15_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591400.1"
                     /translation="AFASHDINGIAGFEDRRQLAWQGVSLDLRYRFLDRDAAPFGLTF
                     ALVTHADRIDDMTGAVARRYGTELTLALDRELIPNRVVAAFNLFYEPEWTHLLGTGLA
                     EQESTAGGAVAVMAQIRPGFLIGGEVRYLRKYDGIGLEEFAGQALFAGPTAYLQLSER
                     SRLTVAWSVQAWGRAAGSTAALDLVNFERHQARLIFGVNF"
BASE COUNT          773 a         1331 c         1303 g          773 t
ORIGIN      
        1 ggtgcgccat cgctgctggc ggcgaaggcg ctcggcggcg aaatggacgc cactgtcaat
       61 tattggaatt tctgcgccgc gctggaagcg aagggcttcc ggcggctcgc tggtatcgaa
      121 gatcttctgc cgaagcttgg agccaagggc cgcacggcga tgatcggcta cgtcttcgat
      181 gaagaatggg ggagcgcaaa ccgcgacgcg gttacccgct tcatcgcaat gacccgcgag
      241 gcaaaggaga ttttagcaac gtcagatgcg gaatgggaaa agatcgcgcc gctcacgggt
      301 gccgccgacg ctgcaacctt gcgtgcctac cgcgatcgtt atcgggaagg aattccccgt
      361 cgcccgatcg ccgacgaaga agccgacgcg cgcgttctct accgcgtgct ggcggcgatc
      421 ggcggccgcg aaattgtcgg gcgggcgcaa gagcttgccc ccggcacatt ctatcgcgca
      481 atccccggag actgagatgc tccgtctcct gtcattcacc gtgtttcttg ccacctggtg
      541 gggcgccgcg ctgctcgctg gcggagagaa gctgccgcct cctcccgctg tgctggcggc
      601 aatcgttgcg gaagccaact ccggtgcgct gttcttcaat ctcggcgtga cattggcacg
      661 cgttgccctc gccttcacgt tggcgatgac gctaggagct gcgatcggtt acgtgatggg
      721 ccggatacgc cttgccgatc gactcgggga tccttggctg atcctgctgc tcaatctgcc
      781 ggcgctcgtc gtcatcgtgc ttgcgtatat ctgggccgga ttaactgagg tcgccgcgat
      841 tgcggcggtc gccattaaca aactgcctat tgctgtcgtc atggtgcgcg agggaacccg
      901 tgtactcgac acggcgctcg acgaaatgac ggcggtgttt gcaatcccgc gctggagggc
      961 attccggcac gtcatattgc cgcaactggc gccctacatc gcggccgccg cgcgatccgg
     1021 tctttctctg gtctggaaga tcgtcctggt tgccgaattg ctcggacgcc caaatggcgt
     1081 tggcttcgag atcggcgtcg ccttccagct gttcgacatc cctcgcctgc ttgcctatgc
     1141 gctgactttc gcggcggtgg tgctcgccat tgaaaccgtc ctggtgcagc cgtttgaaac
     1201 ccggctatct aggtggcggc cccgtgcagc ttgaggtcga tatcgcacgc aaggcgttca
     1261 caaacgcggc cgacgagcgg cacgacgtgc tcgccgattt caactttgcg ctcggcgccg
     1321 gagaggttgc cgtgttcgtc ggcccatccg gctgcggcaa aaccaccatg ctgaagatcg
     1381 tggccggcct cgaccacgac tatcagggcc gcgtcgcctg tccggcaagc gcacgggtag
     1441 gcatggtgtt tcaggagccg cggctgctgc cctggcggtc ggtcgatgac aacgtgcggc
     1501 ttgccgcgcc gaaggctagc gatgaaaagc tctccgcgct attcgcggtt ctggaactga
     1561 ccgcccaccg caaccatttt cccggagaac tatccctcgg cctcgcccgg cgggtcgcgc
     1621 tcgcccgtgc gttcgcgatc gagccggatt tcctcatcct cgacgaaccg ctagcatcgc
     1681 tcgacgacgc cctctccgcc cgcctgcgcg accagatagc cacgttggtg gacagccgat
     1741 ccgtgacgac actgctcgtc acccacgaca tggacgatgc gattcgcctc ggcgacaggg
     1801 tgtttctgct gtcgccacga ccggcgcgga ttctcgccga actgccaatt cgcacaccac
     1861 gcaacgcgcg cggcgaagcc gagatcgctg cgatcaaagc agagatcgca cggcggacca
     1921 atggcgattg cgcgaaggga gatgtcttat aggcagcgct tgcgtcgcgt gttaaaatac
     1981 gtgtcggagg agttggccat gaaacgtgcg tttggaggcg cagcgctcag ccttagtctt
     2041 ctcgccgtga ccgcgatggc gcaggacatg atgcgcggcg tcgatctgtc gtcgcccgac
     2101 atggtctcgg ccgaaatgac ccgcggggaa gtcgaagccg cgctcatcat ggcgagcggc
     2161 gccgcgcccg ccgatttcac cggaaagagg ttgtccagcc tcgacctctc cgggctcgac
     2221 ctgtccggcg cgatttttcg cgcagcacgg ctcaacaaga ccaagcttgc cggcgcgaag
     2281 ctcgatcgtg caatcctcga ccaggcatgg ctgctccaag cggacctgac gggcgcaagc
     2341 ctgaagggcg cgaatctgtt cgcctcgcag atgctccgcg cccggctcga cggcgccgat
     2401 ctctcgcaag cgcgcgttgc ggccgacctt accggcgcga gcctcgtcgg agcctcgatc
     2461 gccggcgcga acctgggcgc cgacatgcgc aaccagtcga tgggcctcat gcgcgcggtg
     2521 ctgaaatccg ccaatctgga gcggctgaac gcacgcggcg ccgacctgtc tcgcgtcgat
     2581 ctcgaattcg cttcgctgaa gggcgccgac ctgacaggtg cttccctcaa gagcgcccag
     2641 ctcgggggag ccgacttgac cggtgtcaca gtcatcggca ccgatttcga cggtgccgat
     2701 ctcgcttcag cgaagttgat cgctccaatt ggcctcgacc aggcaaaaaa tttcgacaag
     2761 gcgaaaaatc gcgatcgtct gataaggcaa tgacggctcc aagcgttcca ccacgggagg
     2821 attgcctgat gcgatggatc ctggcttcgg tcgtgctgtt gttagcgagc gccggcgaac
     2881 ctgccgcgca gggcaagggc atccggttct ggaacctgac caccgcgacc atttccagtt
     2941 ttcaactgtc tccagccggg aaggacacct gggggcctaa ccagaccttg aacgacaagg
     3001 acaaggaagt cgatcatgac gagcggctgc gcattaccgg cgtcgagccg gggcgctacg
     3061 acgccaggat cagctatcgc gacaagcgga aatgcttggt gcgggacatc gagatcaagg
     3121 cagattcggt gttctcgatc acggacaagg atttgatgga ctgcagcaag taacgctgtc
     3181 gcgacggcct tttatgccca gcgcgaggat catcccgcgg gggtcgctcc gcgcgcgtcg
     3241 gccttatcgt tgctgtgcgg atactcgcag cgccaccgca cggcgttcca ttttggatgc
     3301 tcaccgaccc actgggcgac gtagggcggc gcgcccatcg cacactgctg cagtgagacg
     3361 gctccggaaa acatcagatg agtctcttcg caggtcgccg gcgatagcac cgcgcagacc
     3421 gtcacaatca gatcgattgg attcatgccg aacacccttt gcttcggagc cgctgtttag
     3481 cacaaacaga aacggcgatg gcggggcgga gtttcaaaaa gctgagaaat cctgcctctg
     3541 ataccgccag cgcaggaccg cgaggacctg ggaagtcaga aattaacccc gaatatcagc
     3601 cgggcctggt ggcgctcgaa attgacgaga tccagggctg ccgtcgatcc ggccgcccgg
     3661 ccccatgcct gaacgctcca ggcgaccgta agcctggagc gctcagacag ctgcaggtag
     3721 gcggtcggcc cggcgaacag cgcctgcccg gcaaactctt ccaaaccgat accgtcgtat
     3781 ttcctgaggt agcgcacttc gccgccgatg aggaaacctg ggcggatctg cgccatcaca
     3841 gcgacggcgc ccccggccgt cgattcctgc tcggccaatc cggtgccgag gaggtgtgtc
     3901 cattccggct cgtagaagag attgaacgca gcgaccacgc ggtttgggat taattcccga
     3961 tcgagcgcca gcgtcagctc ggtgccgtat ctccgggcaa cggctcccgt catgtcatca
     4021 atccggtccg catgggtcac cagcgcgaat gtcagaccga acggcgccgc atcccggtcc
     4081 aaaaacctgt agcgcaagtc aagggaaaca ccttgccaag ccaattggcg gcggtcctca
     4141 aaaccggcga tgccgttgat gtcatgagac gcaaaggcgc
//
LOCUS       DASWGM010001007         3761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_532595,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001007 DASWGM010000000
VERSION     DASWGM010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532595"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..1391)
                     /locus_tag="VGA15_27115"
     CDS             complement(258..1391)
                     /locus_tag="VGA15_27115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX9591401.1"
                     /translation="MNEATRIRPATAGELGASGEEFQNLHEFVRKARGNLNQNAWDYI
                     VGAAETETTMRRNRMALDEIAFRPRVLRNVARVDASITSFGRKLRLPVMLAPVGALEL
                     FDPGAAASVARGAGAFGAAHMLSSVTEPGLEKVAEAAPDALRIYQLYVRGDDAFVEDC
                     VGRAMANKYAAFCLTVDTAHYSRRERDIAKRYVRASRLRATGGDFQKGLEWRTVKLIK
                     DKYKIPLVIKGIATAEDAAIALDHGVDWIYVSNHGGRQLDQGRGSMQVLPEIVAAVAG
                     RAKIMVDGSFCRGTDIVKAIASGADLVGIGRLQCWALAAAGEAGVLRMLELLEDEVIR
                     CLGLLGVRNFAELDKAYLHAATPTNLPHVFSAFPLLEIEPYRY"
     gene            1773..1970
                     /locus_tag="VGA15_27120"
     CDS             1773..1970
                     /locus_tag="VGA15_27120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591402.1"
                     /translation="MLFSPLTIQEIENPPGDAALKLSGDGPFRNVPPQFGYWLGTCAR
                     RGFGTQPSTRQNGLDDIRRAL"
     gene            2076..3410
                     /locus_tag="VGA15_27125"
     CDS             2076..3410
                     /locus_tag="VGA15_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX9591403.1"
                     /translation="MRKNSLFFLGAATGACLTFLVSGPQGAQLVAAARAAAGTDTYSQ
                     LNLFGDVFERIRTNYVEKPDDAKLIEASISGMIAALDPHSRYMNEKAWRDMQETTSGE
                     FGGLGIEVTMEDGLVKVVAPMDGTPAARAGILSGDLITHINDEAVQGLSLEEAVGKMK
                     GPIDTKTQLKIVRKGVDKPIDLSIVREIIRVRPVSYHADGGDIGYVRITAFNEQTTDG
                     LKKAIAEISKQIPPEKLAGYVVDLRNNPGGLLDQAVSVSGTFMARGEIVSTRGRTAEE
                     TQRFSARTGDLIKGKPLVVLINGGSASASEIVAGALHDHKRATLIGTRSFGKGSVQTI
                     IPLGTGNGALALTTARYFTPSGHSIQAQGIKPDIEVLQEVPDELKGRAELKGEASMHG
                     HLSAEGAEQAGSQSYVPPAEKDDKALAAAYNLLRGVTVNAGVPSSPKTAVPN"
     gene            complement(3413..>3761)
                     /locus_tag="VGA15_27130"
     CDS             complement(3413..>3761)
                     /locus_tag="VGA15_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX9591404.1"
                     /translation="GTGYSSLSYLQRFPFDKIKIDRCFVNDIAEPDGSSCIVQAVVNI
                     AAARHMTTTAEGVETQQQLELLRALGCAEMQGYLFSPAKPAAEIRQLFHRARSAAVNL
                     SPGRKRRQIPNPA"
BASE COUNT          721 a         1181 c         1184 g          675 t
ORIGIN      
        1 ccctggtttt cccagaactt gatgctcaga ggcgcatagg tcttcaaagc cacatccggc
       61 tgcaagagat tcggaaacag tgtcacgtta tcgctcatga ccatcctccg tttggggttt
      121 cctgccacgc cgacacaaat agccgttcag gtccggaccg gatgttcaat atcgcacagt
      181 cgcaacaaag attcggcgcc gccggttccc ctgtcagccc tccggtcatg acgccccgat
      241 cgcgcccagc gccgccatca atagcgatac ggctcgatct ccagcagcgg aaacgcgctg
      301 aatacatggg gcaggttggt cggcgtcgcc gcgtgcagat aggctttgtc gagttccgcg
      361 aaattcctga cgccgagcag acccaggcac cggattacct cgtcttcgag cagttccagc
      421 atccgcagga ctccggcttc gccggcagcg gcaagcgccc agcattgcag gcggccgatg
      481 ccgacgagat cggcgcctga cgcgatcgct ttcacgatat cggtaccgcg gcagaacgag
      541 ccgtcgacca tgatcttggc acggccggcg acggcggcca cgatctccgg cagcacctgc
      601 atcgagccac ggccttgatc gagctggcgt ccgccgtggt ttgacacgta gatccagtct
      661 accccgtgat cgagcgcgat cgcggcatct tccgcggtcg cgatgccctt gatgaccagc
      721 gggattttat acttgtcctt gatcagtttt accgtgcgcc attcgagccc cttctggaag
      781 tcgccgccgg tggcgcgcag ccggctggcg cggacgtaac gcttggcgat gtcgcgctcg
      841 cgccggctgt aatgcgcggt gtcgacggtc aggcagaacg cggcatattt gttggccatc
      901 gcgcgaccga cacaatcctc gacaaaggcg tcgtcgccgc ggacgtagag ctgatagatg
      961 cgcaacgcgt caggcgccgc ctcggcgacc ttctccaggc caggctcggt cacggagctc
     1021 agcatatggg cggcgccgaa ggcgcccgcg ccacgcgcga cggaagcggc ggcgccggga
     1081 tcgaacagct cgagcgctcc gaccggcgcc agcatcaccg gcaggcgcaa tttgcggccg
     1141 aacgatgtga tcgacgcatc gaccctggcg acattgcgca gcacccgcgg ccggaacgcg
     1201 atctcgtcca gcgccatgcg gttgcggcgc atcgtggtct cggtctcggc ggcgccgaca
     1261 atgtagtccc aggcgttctg gttcaggttg ccgcgcgctt tgcgcacgaa ctcgtgaagg
     1321 ttctggaatt cctcgccgct tgcgccgagt tcgccagccg tcgccggccg gatgcgggtc
     1381 gcttcgttca tagtatcctc tcccggaaaa cgcgtgggtt gttcttgggc ttggcctttc
     1441 gcgtatcgct tccggagctt ccgcgccatc gcaaaaatcg gtatggctgg tatcagcggg
     1501 acccaagcaa agaatcccgg cctgcccgaa ggggaggaat tgccatgtcg cgcagccagt
     1561 ggccggcgat catcggtcgc gatggcgcac atccagttcg gttagggccg attacaaatt
     1621 ggacagcaaa cgcccgattt cgggctttgc cggcgtcgcg gagcacgtat atcgtctcgc
     1681 tctggaagac cggccggccc gaagccggga taacggggtt atccagagat aggtccgacg
     1741 ccgaacgggg aaatgataag aggtctgaga cgttgctgtt ttcacctttg actatccagg
     1801 aaatcgagaa ccctccgggg gatgccgcgc tgaaacttag cggcgacggt ccgttccgca
     1861 acgttccgcc gcaattcggc tattggctgg gcacctgcgc caggcgcggt ttcggcaccc
     1921 agccctcgac gcggcagaac ggcctcgatg acatccgaag ggcactgtga gttaaaggca
     1981 ctgtgagtta atgggtcgag ttgagcaccg cgtaagacac cgatttctcc tgcccgtggc
     2041 gggcggcaac tgacagcaag aaggtttggc gaacaatgcg caagaactct ctctttttcc
     2101 tcggcgcggc aaccggtgca tgcctgacct ttctggtcag cgggcctcag ggcgcgcaat
     2161 tggttgcggc tgccagggct gccgccggga ccgacaccta ttcgcagctc aatctgttcg
     2221 gcgacgtatt tgagcggatc aggaccaact atgttgagaa gcccgacgac gccaagctga
     2281 tcgaggcgtc gatcagtggc atgattgcgg cgctggatcc tcactcgcgg tacatgaatg
     2341 agaaggcctg gcgcgacatg caggagacca ccagcggcga gttcggcggg ctgggcatcg
     2401 aggttacgat ggaggacggc ctcgtcaagg tagttgcgcc gatggatgga acgcccgccg
     2461 ccagggccgg catcctgtcc ggcgacctga tcacccatat caatgacgaa gcggtccaag
     2521 ggctcagcct ggaggaggct gtcggcaaga tgaaggggcc gatcgacacc aagacccagc
     2581 ttaagatcgt ccgcaagggt gtcgacaaac ccatcgacct ttcgatcgtg cgcgaaatca
     2641 ttcgcgtgcg cccggtcagc taccacgccg acgggggtga tatcggctat gtcaggatca
     2701 ccgcattcaa cgaacagacc accgacggat tgaaaaaagc gatcgccgag atatcgaaac
     2761 agatcccgcc ggaaaagctg gcgggctacg tcgtcgactt gcgcaacaac ccaggcggac
     2821 tgctcgatca ggcggtctcg gtgtccggta cattcatggc gcgaggcgag atcgtctcga
     2881 cgcggggccg caccgccgag gagacccaac gtttcagcgc gcgaaccggg gatctcatca
     2941 agggcaaacc gctcgtcgtc ctgatcaacg gtggatcggc atccgcatcc gagatcgtgg
     3001 ccggtgcact gcacgatcac aagcgcgcga ccctgatcgg gacccgctcg ttcggcaagg
     3061 gctcggtaca gacgatcatc ccgctcggca ccggcaatgg cgcattggcg ctcacgaccg
     3121 cgcgctattt cacgccgtcc ggccactcga tccaggccca gggcatcaaa cccgacatcg
     3181 aagtattgca ggaagtgccc gacgagttga agggccgcgc ggaactcaag ggagaagcct
     3241 cgatgcacgg ccacctctcc gccgaaggcg cagagcaggc cggctcgcag tcctacgttc
     3301 cgccggcaga gaaggacgac aaggcgcttg ccgcggccta caacctcctg cgcggtgtca
     3361 ccgtcaatgc cggtgtgccg tcatctccca agaccgcggt gccgaactag attcacgcag
     3421 ggtttggaat ttgccgacgc tttcggcccg ggctcaggtt cacggccgcc gatcgtgcgc
     3481 ggtggaatag ctgcctgatt tcggctgccg gcttggccgg actgaacagg tagccctgca
     3541 tctcggcgca gccgagcgcg cgaagcagct cgagctgctg ttgcgtctca acgccttccg
     3601 cggttgtcgt catgtgacgc gccgcggcga tgttgacgac cgcctgcacg atgcaggatg
     3661 acccgtccgg ctctgcgatg tcgttgacga agcagcggtc gatcttgatc ttgtcgaacg
     3721 gaaagcgctg cagataactc agcgaggaat agccggtgcc g
//
LOCUS       DASWGM010001008        12845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_667937,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001008 DASWGM010000000
VERSION     DASWGM010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12845
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667937"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2200
                     /gene="uvrB"
                     /locus_tag="VGA15_27135"
     CDS             <1..2200
                     /gene="uvrB"
                     /locus_tag="VGA15_27135"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX9591405.1"
                     /translation="FPDNAVEYFVSYYDYYQPEAYVPRTDTYIEKDSSINEQIDRMRH
                     SATRALLERDDVIIVASVSCIYGIGSVETYTAMTFALKKGERIDQRQLIADLVALQYK
                     RTQADFTRGTFRVRGDVIDIFPAHYEDRAWRVNLFGDVVENIEEFDPLTGHKQDELEF
                     IKIYANSHYVTPRPTLVQAIKSIKSELKWRLDQLNNQGRLLEAQRLEQRTTFDLEMME
                     ATGSCAGIENYSRYLTGRRPGEPPPTLFEYVPDNALVFADESHVTVPQIGGMFKGDFR
                     RKATLAEYGFRLPSCMDNRPLRFEEWDMMRPQSVAVSATPSAWELNESGGVFVEQVIR
                     PTGLIDPPVNIRPARTQVDDLVGEVRATAQAGYRSLVTVLTKRMAEDLTEYLHEQGIR
                     VRYMHSDIDTIERIEIIRDLRLGAFDALVGINLLREGLDIPECALVAILDADKEGFLR
                     SETSLIQTIGRAARNVDGKVILYADQMTGSMERAIAETDRRREKQVEYNTINGITPES
                     IKKSIGDIMNSVYERDHVLVEIGDGGKGNSWSDDVISIGHNFEAVLGDLETRMREAAA
                     DLNFEEAARLRDEVKRLRATELAVVDDPTAKQRTVQGKAGAYAGTKKYGEAANLPRGS
                     SKRGGNNTPRSSSPSPLRGGSTAKGGRGGGTGEDSSFASRVHKPHLDEMHGPESLPYR
                     PGRATPRKPAPDDIEPGGGSKIFQPTSSREAVLGRSTGGAPGHRGGWKKR"
     gene            2355..2630
                     /locus_tag="VGA15_27140"
     CDS             2355..2630
                     /locus_tag="VGA15_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591406.1"
                     /translation="MAWFLNFYRCARCKRIWTDQWSSMCDDDCPHCGARHMSPYDSEA
                     LTTLIAEDGKEFVVFWSPETAEHDPDYRELGRFPSREKALEFLSADG"
     gene            complement(2947..4074)
                     /locus_tag="VGA15_27145"
     CDS             complement(2947..4074)
                     /locus_tag="VGA15_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9591407.1"
                     /translation="MKLGHLVASGLFLGLFLGLPLSLPAAAHTPQQPPHQLFGEGDLR
                     LESGEAIKDFSISYVTHGTLNAKKSNAILMVTAVGGNHHRLDFMIGPGKALDPDKYFI
                     ICTDAIANGFSTSPSNSKSQPRMSFPRFTIRDMVESQYRLLKEKFGIDHVVAVVGPSM
                     GGMQTLQWGVSHPDFMDALVAMVPLAKTPAWSVAVVEASRKAIMNDPAWKDGNYDAPP
                     EKGVRLWRDIVSLLSARTPDMYSAQFKNGMDALPWMEAQETAALKAFDANDWIYQTFA
                     YERHDVGTTPGFNGDTAKALASIKARTLILTGTKDLLNPEFEPQEAGKNITNVKMMTI
                     SPGTVTGHASAGGAGAADVEFLNREAGAFLDGVTDRGKRLD"
     gene            4321..4467
                     /locus_tag="VGA15_27150"
     CDS             4321..4467
                     /locus_tag="VGA15_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591408.1"
                     /translation="MAKGQMRSNKEKKKPKADKNLKKGGAAPVNPFAAGKTPAGQSPN
                     SKKS"
     gene            4511..4927
                     /locus_tag="VGA15_27155"
     CDS             4511..4927
                     /locus_tag="VGA15_27155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591409.1"
                     /translation="MLDGGAGQARRLETVWRGASTTRATCIVAAAASFCQLVDDADLR
                     VIDSVSFEPSPAGLGSRHRDAKACRTRVLLLAAFSRPDAVTTFTDAIRRNSKRKQQRH
                     LPAARHPARNLKIQLWVMNHFMSRNAKRVHKAAMFC"
     gene            5026..5151
                     /locus_tag="VGA15_27160"
     CDS             5026..5151
                     /locus_tag="VGA15_27160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591410.1"
                     /translation="MLKFLDAFYSGDIDAAQACCDDEFDSITYAPIAARVYLPRS"
     gene            5219..5485
                     /locus_tag="VGA15_27165"
     CDS             5219..5485
                     /locus_tag="VGA15_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591411.1"
                     /translation="MKIALLILLGLVLGVLGGAALGVGAGLAWVEIFKTTSFEGYGGM
                     LVFFTFLPLGALIGGLGGALLFGVIAIRDAEIAIEKDPSRRHDG"
     gene            5648..6082
                     /locus_tag="VGA15_27170"
     CDS             5648..6082
                     /locus_tag="VGA15_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9591412.1"
                     /translation="MTEHSLWGFSRALYRAINERQCEELEALIDDDVEWAIYGPIDMF
                     PFLGARRGRAAVLEVIRQIAENVRVHRFDRESIMLGVDSAASMMRYSLTALDSNKPIS
                     LRLAHFAQFKTGRLVSIRVLVDTFDLVEQALGHPINLPKLAS"
     gene            6238..7206
                     /locus_tag="VGA15_27175"
     CDS             6238..7206
                     /locus_tag="VGA15_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591413.1"
                     /translation="MTLRTNITPRTLYDRGFALLVAAGVSMVLAAPASAQAVPSGEEP
                     DAAEAAAPDAGDADNKDADIKNLELDWSLLNVDASTLTAGPASKARPAPRADTGADLS
                     WSSKDKPNGSAAVSVKQPVSPFLDARVGADMTVVDQSQTLTSSDLLRQKFSTDAPPSQ
                     STGTAWAAITAPGVASIWDKTAVEARVDPSQEQSKLGTSLSKSLPLNEQYSLTLQNGY
                     NVVEQGHSSLSCADCVHLSAVPVRGIAGRPVRNYGTEQSAKLSIADTGTSFIAGQTLS
                     SADDKWLRKIGAEQKLFGEVSISGSIGETAQGTSNKSLTAGFKRSW"
     gene            7371..7790
                     /locus_tag="VGA15_27180"
     CDS             7371..7790
                     /locus_tag="VGA15_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591414.1"
                     /translation="MNVIRPDKIDGIDGDENLAAVTEVEAGIRDFVRNDVAYLRRPAP
                     GLLGSTEPALEPSTEATVNNVNSLIQRVAGTSLSEIENLISELEGLRDLLHAEGQRVQ
                     REISGYAQLSQQAMKSTRMIADNVSQWKRAADGLRNS"
     gene            7983..8120
                     /locus_tag="VGA15_27185"
     CDS             7983..8120
                     /locus_tag="VGA15_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591415.1"
                     /translation="MQSIRPDNTDPIPLRTSPHRMSTIMIRFVGLLFVAVVILALAWS
                     R"
     gene            complement(8175..8318)
                     /locus_tag="VGA15_27190"
     CDS             complement(8175..8318)
                     /locus_tag="VGA15_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591416.1"
                     /translation="MADRSGLKFVGFIFATVTLAVMLTATMVVKGYADGAYSLEATTV
                     ASQ"
     gene            complement(8499..8981)
                     /locus_tag="VGA15_27195"
     CDS             complement(8499..8981)
                     /locus_tag="VGA15_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9591417.1"
                     /translation="MRFFEDIEIGQRREVGSFTFTADGIKKFAGQFDPQPFHLDEEEG
                     RKSLFGGLAASSWHVASACMKLLVADGQRQAQEALARGEKIAIWGPSPGFRELLWLMP
                     VLAGDTIGYASEVYSLRASEKRPEWGILQARNTGTNQRGEPVFSVLATAFVPRRNAGS
                     "
     gene            complement(8981..9448)
                     /locus_tag="VGA15_27200"
     CDS             complement(8981..9448)
                     /locus_tag="VGA15_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9591418.1"
                     /translation="MTLAFEDFPPGHFGSYGPRRVTREEMLAFAAEFDPQPMHLDEEA
                     ASRSMLKGLSGSGWFLCSIMMRMLFDGFIGRTASLGSPGVSELRWLAPLRPGDDLTLE
                     VEVAEARVSRSRPDTGIVTFKGAARNAAGAVLCEMVTPIIVRRRAELALGQAG"
     gene            9663..9956
                     /locus_tag="VGA15_27205"
     CDS             9663..9956
                     /locus_tag="VGA15_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HEX9591419.1"
                     /translation="MFEFRDLFQWDRFITPTIIKTFYWLVLALIGLFGLSGIFSGLAA
                     MAISPFAGFILLLSSIAGVFVGIVFSRIAAEFILIVFRINEHLGAIRDQGQMN"
     gene            complement(10139..11236)
                     /gene="ychF"
                     /locus_tag="VGA15_27210"
     CDS             complement(10139..11236)
                     /gene="ychF"
                     /locus_tag="VGA15_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX9591420.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGE
                     VAVPDPRLDKLAEIAKSGQIIPTRLTFVDIAGLVRGASKGEGLGNQFLATIREVDAVA
                     HVVRCFEDSDITHVEGKIAPLADIETIETELMLADLDSLEKRVDNLAKKAKGNDKDAK
                     EQLDLVNRALVLLREGRPARFLARKPEEERAFGMLGLLTSKPVLYVCNVEEGSAATGN
                     DFSKVVMDLASKEGAVAVAISAKIESEIATLSREERAEFLDTLGLEEAGLDRLIRAGY
                     QLLDLITYFTVGPKEARAWTINRGTKAPAAAGVIHTDFEKGFIRAETIAYADYVALGG
                     EAGARDAGKLRLEGKEYVVADGDVMHFRFNT"
     gene            complement(11396..12004)
                     /gene="pth"
                     /locus_tag="VGA15_27215"
     CDS             complement(11396..12004)
                     /gene="pth"
                     /locus_tag="VGA15_27215"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX9591421.1"
                     /translation="MLLFVGLGNPGAKHAHNRHNIGFMAVDEIARRHGFAPWRRRFQG
                     ETAEGMLDRERVVLLRPSTFMNESGRAVQEAASFFKLSEGEITVFQDELELPAAKVRV
                     KVGGGIAGHNGLRSISSHMGNDYRRVRLGIGHPGVKELVHGYVLSDFAKDERPWVVAL
                     CEAIADNAGLLVTDRDSTFQNKVHLAMQAKGFIDKGDDAGGS"
     gene            complement(12026..12796)
                     /locus_tag="VGA15_27220"
     CDS             complement(12026..12796)
                     /locus_tag="VGA15_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX9591422.1"
                     /translation="MATVKELKATTRPAGGKGAARAERRAGRVPGVIYGNNQPPVTIS
                     VEDTELRQRILAGRFLTTIYDIDLEGKKHRVIPRDFHLDPVRDFPLHVDFMRLGEGAT
                     IRVSVPLRIQNAEAAPGVKRGGTVNIVTHTIDLECSVDNIPQYIEADVSGLEISHSLH
                     LSDVKLPTGVRSLSREDVTLVTIVPPSGYAEEVKAAAAAAAAAGPAAVAAPGAAPAAG
                     AAAAPAAGGAAAAGAKAPAAGAKAPAAGAKAPGGDKKK"
BASE COUNT         2482 a         4013 c         3902 g         2448 t
ORIGIN      
        1 cttccccgac aacgccgttg agtattttgt ctcgtattac gattactacc agcccgaggc
       61 ctatgtgccg cggaccgaca cctatatcga gaaggactct tcgatcaacg agcagatcga
      121 ccgcatgcgc cactcggcga cacgggcgct cctggagcgc gacgacgtca tcatcgtggc
      181 ttcggtatcg tgcatctacg gtatcggttc ggtcgagacc tacaccgcga tgacgtttgc
      241 gctgaagaag ggcgagcgca tcgaccagcg gcagttgatc gccgatctcg tcgcgctgca
      301 atacaagcgg acgcaagccg atttcacccg cggcacgttt cgcgtccgcg gcgacgtcat
      361 cgacatcttt ccggcgcact atgaagaccg cgcctggcgc gtgaacctgt ttggcgacgt
      421 cgtcgaaaat atcgaggagt tcgatccgct caccggccac aagcaggacg agctcgaatt
      481 catcaagatc tacgccaact cgcactatgt gacaccgcgg ccgacgctgg tgcaggccat
      541 caagtcgatc aagagcgagt tgaagtggcg gctggatcag ctcaacaatc agggccgcct
      601 gttggaagcg cagcggctcg agcagcgcac caccttcgat ctggaaatga tggaagccac
      661 cggctcctgc gccggcatcg agaattattc gcgctatctg accggacgcc gccccggcga
      721 gccgccgccg accttgttcg aatatgtccc cgacaatgcg ctggtcttcg ccgacgaaag
      781 tcacgtcacc gtgccgcaaa tcggcggcat gttcaaaggc gatttccgcc gcaaggcgac
      841 gctggccgaa tacggtttca ggctgccctc ctgcatggac aaccgtccgc tgcggttcga
      901 ggagtgggac atgatgcgcc cgcaatcggt cgcggtgtcg gcgacgccga gcgcctggga
      961 actgaacgag agcggcggcg tgttcgtcga gcaggtgatc cggccgactg gcctgatcga
     1021 tccgccggtg aatatccgtc cggcccggac ccaggtcgac gatctcgtcg gcgaggtccg
     1081 cgccaccgcg caagccggct atcgctcgct cgttaccgtc ttgacaaaac ggatggcgga
     1141 agacctcacc gaatatctgc acgagcaggg tattcgtgtg cgctacatgc actctgatat
     1201 cgacaccatc gagcgcatcg agattattcg ggatttgcgg cttggcgcgt tcgacgccct
     1261 ggtcggcatc aatctgttgc gcgaaggcct cgacattccc gaatgcgcgc tggtcgcaat
     1321 cctcgatgcc gacaaggaag gctttttgcg cagtgagaca tctctgatcc agaccatcgg
     1381 ccgcgccgcg cggaatgtcg acggcaaggt gatcctctat gccgaccaga tgacgggatc
     1441 gatggagcgc gccatcgccg agaccgaccg ccgtcgcgaa aagcaggtcg aatacaacac
     1501 catcaacggc atcacgccgg aaagcatcaa gaaatccatc ggcgacatca tgaacagcgt
     1561 ctacgagcgc gaccatgtgc tggtggaaat cggcgacggc ggcaagggaa attcgtggtc
     1621 cgatgacgtc atctccatcg ggcataattt cgaggcggtg ctcggcgatc tcgaaacccg
     1681 gatgcgcgag gccgccgccg atctgaattt tgaggaagcc gcgcggctgc gcgacgaagt
     1741 caaacgcctg cgcgccactg aactcgcggt agtcgatgat ccgacggcaa agcagcgcac
     1801 ggtgcagggc aaggccggcg cctacgccgg cacaaagaaa tacggcgagg ccgcgaattt
     1861 gccgcgcggc tcatcgaagc gcggcggcaa caacaccccg cgctcctctt ctccctcccc
     1921 cttgcgggga gggtcgaccg ccaaaggcgg tcggggtggg ggtacaggcg aagactcttc
     1981 tttcgcttcc cgcgtccaca aaccccacct cgacgagatg cacggcccgg aatccctgcc
     2041 ctaccgtccc ggccgcgcca cgccgcgcaa acccgcgccc gacgacatcg aacccggcgg
     2101 cgggagcaaa atcttccagc cgacttcatc gcgcgaagcc gttctcggcc gctccaccgg
     2161 cggcgcgccg ggacaccggg gcggatggaa gaagcggtag cctgtcaaaa attcgcaacg
     2221 cgaaacatcg ctctgcgcat acgcgaagag agatgcatat tgcgcacgtt gcaacgcggc
     2281 gaattacccg gctgcggatt tattgatttc gtcgcacgaa gcaattatcg tttgcaaatc
     2341 tcaggacggt tcgcatggcc tggtttctga atttctacag atgcgcacgc tgcaagcgga
     2401 tatggaccga tcaatggtcg agcatgtgcg acgacgattg cccgcattgc ggggcgcggc
     2461 acatgtcgcc ttacgatagc gaggcgctga ccacgctgat tgcagaggac ggcaaggaat
     2521 tcgtcgtgtt ctggtcgcct gagacagcgg agcacgatcc cgactatcgc gaactcggcc
     2581 gcttcccgtc gcgcgagaag gccctggagt tcttgtccgc tgacgggtga cctctacgtt
     2641 atggttcgtc tatgctttct gtcatcatcc gccaccgggt ctcgccgtcg gcgagcccga
     2701 tgacaggctc cagcggatga tccagtattc cagcgcgcta gcgattggac cgggaagccg
     2761 cgacgtaccg gatacccccg ctttcgcggg gtatgatggc cagttgtgga gcgacgtagt
     2821 gcgttacaat acacggcgtc atacgcgggc ttgacccgcg tatccatccc ctcgcaagag
     2881 tcgtccgaag aggatggatt gccgggtcga gcccggcaat gacgatggtt gtcggcgacg
     2941 ctcgctctaa tccaacctct tcccgcggtc ggtcacgcca tcgagaaacg cgcccgcctc
     3001 gcggttgaga aattcgacat ccgccgcacc ggcgccgccg gccgaggcgt ggccggtcac
     3061 ggtgccgggg ctgatcgtca tcatcttgac gttggtgatg ttcttgccgg cctcctgcgg
     3121 ctcgaattcc ggattgagca gatccttggt gccggtgagg atcagcgtcc tggctttgat
     3181 cgaagccagc gccttcgcag tgtcgccatt aaagcccggg gtggtgccga cgtcgtggcg
     3241 ctcataggca aaggtctggt agatccagtc gttggcgtcg aacgccttca gcgcggcggt
     3301 ctcctgcgcc tccatccagg gcagcgcatc catgccgttc ttgaactgcg ccgagtacat
     3361 gtccggcgtg cgcgccgaca agagactgac aatatcgcgc cacagccgca cgcccttctc
     3421 cggcggcgca tcgtaattgc cgtccttcca ggccggatcg ttcatgatcg ccttgcgcga
     3481 ggcttccacc accgcgacac tccaggccgg cgtcttcgcc agcggcacca tcgccaccag
     3541 cgcgtccatg aaatcgggat ggctgacgcc ccattgcagc gtctgcatgc cgcccatcga
     3601 cggcccgacc acggcgacca catggtcgat gccgaatttt tccttgagca ggcgatattg
     3661 cgattccacc atgtcgcgga tggtgaatct cgggaaactc atacgcggct gcgatttgga
     3721 attgctgggc gacgtcgaaa acccgttggc tatcgcatcg gtgcaaatga tgaaatattt
     3781 gtcgggatcg agcgccttgc cggggccgat catgaagtcg agccggtgat gattgcctcc
     3841 caccgccgtc accatcaata tggcgttgga tttcttggcg ttgagggtgc cgtgggtgac
     3901 gtaggaaatc gaaaaatcct tgatcgcctc gccgctttcg agtctgagat cgccttcgcc
     3961 aaaaagctgg tgcggcggtt gctgcggcgt atgcgccgcg gcgggcagcg acaacggcag
     4021 gcccaggaac aggcccagga acaatcctga ggccacgaga tgtccgagtt tcactttcaa
     4081 aatcctcccg aaaatggagg gactttgacg gccgccctcc tcgttcgaac gatacgggcg
     4141 cattggatcg ctgtgaagcg acatgccgtg acgcccggca aagatttgcc ggggcgcggc
     4201 atgcggaata aatcgaggtc ccagcggttt cgcgtccaaa ccgccgcata caaatcttcc
     4261 gagatttgct attcctacgc cgcgcccaat ctcgtcaacc ggggcgccag ggagatcgtc
     4321 atggccaagg gtcagatgcg cagcaacaag gaaaagaaaa agccgaaggc cgacaagaac
     4381 ctcaaaaagg gcggcgccgc gccggtcaat ccgttcgcgg cgggaaaaac cccggccggc
     4441 caaagcccga acagcaagaa gagctgaggt tcaggtctga gccagtctcg ctgaatcaga
     4501 cccgtcgctt atgcttgatg gcggcgccgg ccaagcccgg cgcctcgaaa ccgtatggcg
     4561 cggcgcctca actacacgcg caacttgcat cgtcgcggcc gcggcgtctt tttgccagct
     4621 ggtcgacgac gctgacctgc gtgtcataga cagcgtcagc tttgagccat caccggccgg
     4681 ccttgggtcg cggcaccgcg atgccaaagc ctgtcggacg cgggtcctgc tgcttgctgc
     4741 gttttcgcgg cctgatgcgg tcacgacatt caccgacgcc atccgcagaa actccaaacg
     4801 gaagcagcaa cgccatctgc cggcggcacg gcatcccgcg cgcaacctta aaatacaact
     4861 ctgggttatg aaccatttca tgtctcgcaa cgccaaacga gttcacaagg ccgcgatgtt
     4921 ttgctgattg agacctgcag aacggctttc acaaaggacc gacaatgcaa gtcgggcgta
     4981 accccggcgg agaaatggat ggtcgaggct cttcaccgat agcgcgtgct gaaatttctc
     5041 gatgcgttct attccggcga cattgatgcg gcccaggcgt gctgcgacga cgagttcgac
     5101 agcatcacct acgcgccgat cgccgcccgg gtttatttgc ctcgtagttg aacttggccg
     5161 tgtcctgccg cgacaaacgc gaggccccta tggggcatcg acaggcaaaa tcacctccat
     5221 gaaaatcgcg ttgcttatcc tgctggggct ggtgctgggc gtgctcggcg gcgccgcgct
     5281 cggcgtcggc gccgggctcg cctgggtcga aatcttcaag accaccagtt ttgaaggcta
     5341 tggcggaatg ctggtgttct tcaccttcct gccgctcggc gccctgatcg gcgggctcgg
     5401 cggggcgctg ctgttcggcg tgatcgcgat ccgcgacgcc gaaatcgcca tcgaaaagga
     5461 tccttcgcgc cggcacgatg gctaaatcgc cggcatttgt gcaatgcaaa aatgcaggtt
     5521 gcattttgat cggacgcgat tatttgaagc ttcaaatatc gaatgcagca tccaggcacg
     5581 gcgtcgccgc tgacgcccaa gagggccgtt tgctgagtaa tcaatccggt ttcgggaaaa
     5641 aaacatcatg acagagcaca gtctctgggg tttttcgcgc gcgttgtatc gcgcgatcaa
     5701 cgaacgccaa tgcgaagagc tcgaagcctt gatcgacgac gacgtcgagt gggcgatcta
     5761 cggaccgatc gacatgtttc catttctcgg ggcgcgccgc ggcagggctg cggtgctcga
     5821 ggtgatcagg cagatcgcgg aaaacgtgcg ggtgcaccgc ttcgatcgcg agtcgatcat
     5881 gctgggcgtg gactcggcgg cctcgatgat gcgctattcg ctgacggcgc tggattcgaa
     5941 caagccgatc agcctgcggc tcgcccattt cgcgcaattc aagaccggcc ggctggtcag
     6001 catccgcgtc cttgtcgata ccttcgacct cgtggagcag gcgctcgggc atccaataaa
     6061 tctgccgaag ctcgcgtcat agtgatcgtc tggtctttca ggtgatcgca gctgtaatgg
     6121 ccccgccatc attttctggc atggtaagcg aatcgacggt gatcagacgt cggtgatcag
     6181 gcgtcggtga tcaggcgtcg gtgatcagac ttgggcggag ctatgactct cggaaacatg
     6241 actctcagaa caaatattac tcccagaaca ttgtatgacc gcggtttcgc gctgttggtg
     6301 gcggccggcg tgagcatggt tctggccgcg ccggcgagcg cgcaggccgt gccgtcaggc
     6361 gaagagccgg acgccgcgga agccgccgca cccgatgccg gggacgccga caacaaggac
     6421 gccgatatca agaatttgga gctggactgg agcctgctca atgtcgatgc ctccaccctg
     6481 accgccgggc cggcgtcgaa ggctcgccct gcgccccggg cggacacggg cgcggacctg
     6541 tcgtggtctt ccaaagacaa accaaatggc tcggccgcgg tatcggtgaa gcagccggta
     6601 tcgccattcc tggatgccag ggtcggcgcc gatatgaccg tggtcgatca gtcccagaca
     6661 ttgacgagtt cggatctgct gcggcaaaaa ttttccaccg acgccccacc atcgcaatcc
     6721 accgggacgg catgggcggc gatcacggct cccggcgttg cttcaatctg ggacaagacc
     6781 gcggtcgaag cgcgggtcga tccttcgcag gagcagagca agctcggcac ttccttgagc
     6841 aaatctttgc ctctgaacga acaatattcg ctcaccctgc aaaacggcta caatgtggtc
     6901 gagcagggcc atagcagtct gtcttgcgca gactgcgtac acttgtctgc ggtgccggtg
     6961 cgcggcatcg ccggccgccc ggttcgtaac tatggaaccg agcaatcggc gaagctgagc
     7021 attgccgaca ccggcaccag tttcatcgcc ggccagacgc tttcgtcggc cgacgacaaa
     7081 tggctgcgca agatcggcgc cgagcagaaa ttgttcggcg aagtcagcat ttccggctcg
     7141 atcggcgaaa ccgcgcaggg gacgtcgaac aagagcctca ccgcgggatt caagcgcagt
     7201 tggtaggcgc cgggtgcaac ctgtgactgt ggctaaatcc ggtgaaagat ccatgccttg
     7261 ccgcatattg gccgcgatgc ggtcaaaatc agcggtgcag atgggggcgg taaaattggt
     7321 cgtgcggggg gacaaccgcg ctcgcgcaac gcgaaacagg ggaataaacg atgaacgtca
     7381 ttcgtccgga caagattgat ggaattgacg gggacgaaaa tctcgccgcc gtcacggaag
     7441 tcgaggccgg gattcgcgac ttcgtccgca atgatgtcgc ctatctgcgc cgtccggcac
     7501 cgggcctgtt gggcagcacc gagccggcgc tggagccgag caccgaggcc accgtcaaca
     7561 acgtcaactc gctgatccag cgcgtcgccg gaacgtcgct gtcggaaatc gaaaatctga
     7621 tttccgagct cgagggcctg cgcgacctgc tgcatgccga aggccagcgc gtccagcgcg
     7681 agatctccgg ctacgcccag ctcagccagc aggcgatgaa gtccacgcgc atgatcgccg
     7741 ataatgtgtc gcagtggaaa cgcgccgcgg acggccttcg caacagctga tcccgaacta
     7801 aaaccaccga caatcaccgc cgcctcccgt ctcgggagcg cggtggtttt atttggttag
     7861 agtgttttct ggcgaatcag aaccaagaag ctctgggacg cttttgaact cgtcgggtcg
     7921 cggacacgac atagagcgga caccgccgca cggcggcaag tttcgggggg ggacaaacct
     7981 tcatgcaaag cattcggcct gacaataccg atccgattcc gttgcggact tcaccgcacc
     8041 ggatgagcac catcatgatc cggtttgtcg gactgctgtt cgtggccgtg gtgattctgg
     8101 cgctggcctg gagccgctga ttttttcgca attctctttg cgatctcggc ttctttggtt
     8161 agcgaaccct gaacctattg gctggcgacc gtcgtggcct cgagcgaata ggcaccatcg
     8221 gcgtaaccct tgaccaccat cgtggcggtc agcatcaccg cgagcgtcac ggtggcgaag
     8281 ataaagccta caaatttcaa gccgctgcgg tctgccatgg tcgtccccgt ttgtccctgt
     8341 ctccacaatg gcagacactg agttcaaaat gcgttagcgg gacaatggtt ccaaacggcg
     8401 tggtgactga cttctaaacg agcgtcgaag ggttaaaatc gtcattgcga gccagcgggg
     8461 gcgtcgcttt gctcctcgca acgacggagc gcaatttttc aggaaccggc gttccgcctt
     8521 ggcacaaagg ccgtcgccag cactgaaaac accggttcgc cgcgctggtt ggtgccggtg
     8581 ttgcgggcct gcaggatgcc ccattcgggg cgcttctccg acgcgcgcag cgaatagacc
     8641 tcgctggcat agccgatggt gtcgcccgcc agcaccggca tgagccacag tagttcacgg
     8701 aagcccggcg atggtcccca gatcgcaatc ttctcgccgc gcgcgagagc ctcctgggct
     8761 tggcgctggc cgtcggccac cagcagcttc atgcaggccg acgcgacgtg ccagctcgac
     8821 gccgcgaggc cgccgaacag cgatttgcgc ccctcttcct cgtcgagatg aaacggctgc
     8881 ggatcgaatt ggcctgcgaa tttcttgatt ccgtcggcgg tgaaggtgaa cgagcccact
     8941 tcccgccgct gcccgatctc gatgtcctcg aagaaacgca tcagcccgcc tgccccaaag
     9001 cgagttcggc gcgccgccgc acgatgatcg gcgtgaccat ctcgcacaat accgcccccg
     9061 cggcgttgcg ggccgcgcct ttgaaggtga cgatgccggt atcgggacgg ctgcgggaaa
     9121 cccttgcctc cgccacctcg acttcgagcg tgagatcgtc tcccggccga agcggtgcga
     9181 gccagcgcaa ttcgctgaca ccgggtgagc ctagcgaggc ggtgcggccg ataaagccgt
     9241 cgaacaacat ccgcatcatg atcgagcaaa gaaaccagcc cgacccggag agccccttca
     9301 gcatcgaccg cgacgcggcc tcttcgtcca aatgcatcgg ctgcggatcg aattcggcag
     9361 caaaggcaag catttcctcg cgggtgacgc ggcgcggacc ataactgccg aaatgaccag
     9421 gcggaaaatc ttcaaaggcg agggtcatgg tgcggattgc ctgaaggagg cctgattgtg
     9481 gccgttattt gcggcaatct caaccgcccc actcgcatgc ctcgcgtgct ctacgggcga
     9541 gcaccccttc ggtgcccgtc ctggacttgc ccgggacaac gttcggagct aggccgtgtg
     9601 ctgccagcag caatgcctgt agttaacccc tttacttatt tgactcgccg ggagaacgac
     9661 cgatgttcga atttcgggac ctgtttcagt gggaccgctt catcaccccc accatcatca
     9721 agaccttcta ctggctagtt ctcgcgttaa ttgggctttt cggcctctcc gggatttttt
     9781 caggtctggc cgcgatggca atcagcccgt tcgccgggtt cattctgcta ttgtcctcga
     9841 tcgccggggt attcgtcgga atcgtgtttt cgcgcatcgc ggcggaattc atcctgatcg
     9901 tgtttcgcat caacgagcat cttggcgcaa tccgcgacca ggggcagatg aattagtctc
     9961 gtctcgtttt tcgttctacc ttagatcgtc atgcccggcc ttgtgccggg catccacgtc
    10021 ttcctttgat ggttgcagaa gaaagacgtg gatggccggg cgcagacaag ttcacgtagt
    10081 ctgcgcaagg cagactacta tgcccggcca tgacgaaacg tacccatctg ctctgcgctt
    10141 acgtattaaa cctgaaatgc atgacgtcgc cgtcggcgac gacatactcc ttgccctcca
    10201 gccgcaattt gccggcgtcg cgggcgccgg cctcgccgcc caatgcgacg taatcggcgt
    10261 aggcgatggt ttcggcccgg ataaaaccct tttcgaaatc ggtatgaatc acgccggcgg
    10321 ccgccggcgc cttggtgccg cggttaatgg tccaggcgcg ggcttccttg ggcccgacgg
    10381 tgaaataggt aatgaggtcg agcaactggt agccggcgcg gatcaggcgg tcgagaccgg
    10441 cctcctccag tccaagcgtg tcgagaaact cggcgcgctc ctcgcgcgac agcgtcgcga
    10501 tctccgattc aatcttggcg gagattgcaa ccgcgaccgc gccttccttg ctggcgagat
    10561 ccatcaccac tttcgaaaaa tcattgccgg tcgccgccga gccttcctcg acattgcaga
    10621 cataaagcac cggcttggaa gtgagcaggc cgagcatgcc gaaggcgcgt tcctcctcgg
    10681 gcttgcgcgc gagaaaccgc gccggtctgc cctcgcgcaa cagcaccagc gcgcggttga
    10741 cgaggtcgag ctgttccttg gcgtccttgt cgttgccttt tgccttcttg gcgaggttgt
    10801 cgacccgctt ctcgaggctg tcgagatcgg cgagcatcag ttcggtctcg atggtctcga
    10861 tatccgccag tggcgcaatc ttgccttcga catgggtgat gtcggaatcc tcaaagcagc
    10921 gcaccacatg cgcgaccgca tcgacctcgc ggatggtggc gagaaactgg ttgccgaggc
    10981 cctcgccctt cgaggcgccg cgcaccaggc ccgcgatatc gacaaaggtc agccgcgtcg
    11041 gaatgatctg gccggatttg gcgatctccg cgagcttgtc gagcctggga tcaggcaccg
    11101 cgacctcacc gacattcggc tcgatggtgc agaacggata attcgccgcc tgcgccgccg
    11161 ccgtttccgt cagcgcattg aacagcgtcg acttgccgac attgggcaat ccgacaatcc
    11221 cgcatttgaa tcccatcgtg cttcccgatc cttcgcgcgc ggcgaatgag tttatttcgg
    11281 agcgtcatac gcgggcaaaa ggcgcgaagc gccgtcttcc cacctaacaa cccgcgtatc
    11341 catcttcttc gaatgttgga tcgccggatc aagtccggcg atgacggcgc gtgctttaac
    11401 ttccgccggc gtcatcgccc ttgtcgataa atcccttggc ctgcatcgcc agatgcacct
    11461 tgttctggaa cgtcgaatcg cggtccgtca ccagcagtcc ggcattatcc gcgatcgcct
    11521 cgcagagcgc gacgacccag ggacgttcgt ccttggcgaa atcggacagc acgtagccgt
    11581 gcaccaactc cttgacgccg gggtggccga tcccgagccg cacgcggcgg tagtcgttgc
    11641 ccatatgcga cgagatcgag cgcagaccgt tgtgtccggc aatgccgccg ccgaccttga
    11701 cgcgcacctt ggcggccggc agttcgagct catcctgaaa cacggtgatc tcgccttccg
    11761 acagcttgaa gaaactcgcg gcctcctgaa ccgcacgccc cgactcgttc atgaaggttg
    11821 aggggcgcag cagcaccacg cgctcgcgat cgagcatgcc ttccgcggtc tcgccctgaa
    11881 aacgccggcg ccatggcgcg aagccatgac gccgcgcaat ttcatcgacg gccatgaaac
    11941 cgatgttgtg ccggttatgc gcgtgtttcg cgccgggatt gccgagacca acaaagagca
    12001 gcatgacgcg gcatcccgcg tccggttact tcttcttgtc gccgccaggc gccttggcgc
    12061 cggccgcggg cgccttggcg cctgccgcag gtgctttggc gcccgccgca gcggcgccac
    12121 ccgccgccgg agcggcggca gcgccggccg ccggagcagc gccgggggca gccacagccg
    12181 ccggaccggc agcagcggcg gcagccgcgg ccgccttgac ttcttcggcg tagccggacg
    12241 gcggcacgat ggtcaccagc gtcacgtctt cgcgcgacaa cgaccgcacg ccggtgggca
    12301 gcttgacatc cgacagatgc agcgagtggc tgatttccag tccgctgaca tcggcctcga
    12361 tatattgcgg aatgttatcg accgagcatt ccaggtcgat ggtgtgggtg acgatgttga
    12421 cggtgccgcc gcgcttgacg ccgggtgcgg cttccgcgtt ctggatgcgc aggggaacgc
    12481 tgacgcggat ggtggcgcct tcgccgagcc gcatgaaatc cacatgcagc ggaaaatcgc
    12541 gcaccggatc gagatggaag tcgcgcggaa tcacgcggtg cttcttgccc tccagatcga
    12601 tgtcgtaaat cgtggtgaga aagcggccgg ccaggatgcg ctggcgtagt tcggtatcct
    12661 cgaccgaaat agtcacgggg ggctggttgt tgccatagat caccccgggc actctgccgg
    12721 cgcgacgctc tgcccgtgcg gcccccttgc cgcctgccgg acgtgtggtc gccttcaatt
    12781 ccttgacggt cgccattgga ttaagtcctt gttttccaaa aagttaaagg ccgcatcgcg
    12841 gccca
//
LOCUS       DASWGM010001009        11671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1631691,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001009 DASWGM010000000
VERSION     DASWGM010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11671
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631691"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VGA15_27225"
     CDS             <1..182
                     /locus_tag="VGA15_27225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX9591423.1"
                     /translation="DDIPHTVASSTRMFKSKALDTDDSYSFTFNEPGSYGYFCSLHPH
                     MTGKIVVEPANHAAQ"
     gene            284..1348
                     /locus_tag="VGA15_27230"
     CDS             284..1348
                     /locus_tag="VGA15_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591424.1"
                     /translation="MAHNHDNHEYGVSRRHAIERMIWVGTGVLWTVSGGVPKSLSLLG
                     RAEAATASAQTKPTIPIIVKDTTSLYWQVVLAGARKAGQDLGVNVAELGAQSESDVDG
                     QIRILENAVASNPAAVVIAPAQFAPLGKPIDEAAKKVKIIGIDSAADSRALTSFLTTD
                     NEQAGRIAADILAEAIKRTYADAEGDVALITSLPGIASLDQRAKGFKEPIAAKYGALD
                     IVAEKVGDGKAATGSKIMMDLIAEHPELRGVFASNLVMAQGAALAVAENGTNKTGDKI
                     YLVGFGLDDDLVRFLQNGTIAALVVQNPFRMGYDGVKIALAASRGEQVPANVDTGANP
                     ITKANMNSARSQELLNPRIK"
     gene            1812..2354
                     /locus_tag="VGA15_27235"
     CDS             1812..2354
                     /locus_tag="VGA15_27235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591425.1"
                     /translation="MKRASALLFFTSAIVSSASLAYAQHATVDDQELMTKLRQAAPAH
                     VLEQATVMNTGHDGKMKVIQEGNNGWTCMDPGGAPMCADKAAMEWVEAWQSKGPAPQR
                     LGFIYMLSGDNGASNTDPYATKETSDNNWIKTGSHVMIVGAEAKSMMQGYPRDAKADP
                     NKPYVMWPGTPYEHLMLPTK"
     gene            2372..3151
                     /locus_tag="VGA15_27240"
     CDS             2372..3151
                     /locus_tag="VGA15_27240"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX9591426.1"
                     /translation="MMEWRDQVAVVTGSARGIGRATARQLAQRGAAVCVNYAAHADAA
                     EALIAEIAATGGRAIAAAADVAEAAAVETMVARTEAELGPVTILVNNAGVQWQGTLDT
                     YDPEMVARMRGVNVDGVIHATRAVMGGMRARRYGRIVNVSSVAAIGTALPGNAFYAAT
                     KAEVVSLTRRFAMELGPHGITVNAVAPGFVRTDMTQRGRGATDWQGTQERLAARAMMG
                     RIGEPEDIANAVVFLVSPESGWITAQMLTVDGGRMDYIGHG"
     gene            3256..3702
                     /locus_tag="VGA15_27245"
     CDS             3256..3702
                     /locus_tag="VGA15_27245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591427.1"
                     /translation="MSFVLCTGPSALRAADCEDTVARHMVGQALLAAHFVALAEKAGM
                     TPGEINAILKSVAEKSVMQEFWITDSAGHAYLTNTGIDFTFSPDSTKAPQASAFWPLI
                     NGSKDIVIQGARKREIDDQIFKYVGVGGVDKARIVQVGVGAGNLCK"
     gene            3791..4900
                     /locus_tag="VGA15_27250"
     CDS             3791..4900
                     /locus_tag="VGA15_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591428.1"
                     /translation="MRGQSLLKRYAYATATFIALTGLGTTTKSQDLPSYMAPISGRTV
                     SSPADTAVKNVLALNTGMFELYDDAAKIFQGNILAKHPVILGLFSGAGGRFILYRPGM
                     APLEAPSVPIVYQLLKSVDHSTMALSQVVGPYLDNPADQSWRGPMLAYRARMQSALAG
                     LDGTSMPPEWRDNDRIILQNNIAFMDDCLANGAISFAALEAFAKKQAPYLKEDIAWAA
                     QIQVAHWMKVLADWKKMLGGDWDKTYAASNSIYATRQNNVLFSVLAQFFGSEAVNDRL
                     VLIETISFTMTPNDMMEALARIIADRSDGALFFGNYYMMDYELMGGDARAAIVAESTK
                     LGTKPFLPPLVPFGSKQWPMRITPGPGPGSIAELP"
     gene            5000..5251
                     /locus_tag="VGA15_27255"
     CDS             5000..5251
                     /locus_tag="VGA15_27255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591429.1"
                     /translation="MITRHRLLETGVGTALVGPGLPWLGAAALAQGRPKLSPLLPVGT
                     RSEAIFDALRGKKPLISAHPGSIACKPIAAPATASISCR"
     gene            5502..6500
                     /locus_tag="VGA15_27260"
     CDS             5502..6500
                     /locus_tag="VGA15_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX9591430.1"
                     /translation="MTEREYGPKNNRKPFGFVINMLLALVCLLISAGPAVSENTGDWI
                     TGLPREPIHVAAWPGGRKIAVCFIFYVEIWGFGHGPNFRSDMVARDPDVVDEAFRQYA
                     VDWGIPRVGRLFDEQGMPLSIALNALFPETRPDLWKQFRSLVPKAPIIAHGINNSTDL
                     LPLGRGLDEQRAYIHRTLDLIEKYTGVRSRGWTSPSVYPNGDTFRATAAEGIAYSLDG
                     MDSDILSRLTTQSGPLVLIPYPAVTVDMGQYLQRLKQPADMERLWIDYVTELAREAEA
                     HPDREATVVAIGIHPFVVGTPDGAAALRRVLENFKNQKLVWVTDVEAVLGVAGEQR"
     gene            6640..7092
                     /locus_tag="VGA15_27265"
     CDS             6640..7092
                     /locus_tag="VGA15_27265"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX9591431.1"
                     /translation="MIAATDVATEDERFMRLAIEEAWQADFPFGAVIVRDGLVLARGR
                     NLGRTNGDPTAHGEMVAIRRFLADHGSAALQGSTLYTSGEPCAMCMGAILWCRIGRLV
                     FAASLQQLATKIDQIMISSVDLAAKTPFVPIAITGGVLADDAMQLFAK"
     gene            7367..8476
                     /locus_tag="VGA15_27270"
     CDS             7367..8476
                     /locus_tag="VGA15_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591432.1"
                     /translation="MKHASTLIGAACALLGSLAITGPGSQAQGAEVPIYLKSIVGTQA
                     ASPAEVGTKNILQLNTSMFELYDGSSKIFQRNFLAKHPVILGLFSGAGGRFILYRPGI
                     APIEAPSVPIVYQLMKSVGHSTMALAEVVMPYLDSPNDHSWRGALAAYRSRMQSALDG
                     LEATPMRDDWRPTNRAILQNNIAFMDECLAKNAISLEALQAFAKKQAPLLKENIAWAA
                     ETQVGHWMNVIAEWKLMLGADWDKTFAASNTIYVARQNNVLFSVLAQYFGPDAINDRL
                     LLVETISFTTTPDEMLEALTRVVADRPVGQIFFGNYHLMDFELMGGDGRRAIVDEDAK
                     RKIDPTLPPLVPWGSHQWPALVTPGPGPKTLADLP"
     gene            8558..9412
                     /locus_tag="VGA15_27275"
     CDS             8558..9412
                     /locus_tag="VGA15_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX9591433.1"
                     /translation="MNLNSVVEVKRPASADEIRQWRDGYAWLAGGTWLFSEPQDATDT
                     LIDLDQLQWPALEASSAGLDIAATCRIAELYRFEGPAEWIATPLLRECCNCFLASFKI
                     WNAATIGGNICMSLPAGPMISLAAALEATLTLWPRDAAPREVPAVDFVTGNHTNVLQR
                     GELLRNIHLPARALSGRFALRRASLTQLGRSAALVIGTRGVHSDDDLLITITATTPRP
                     LQLRFDRFPSANDLRQAIDARIPADSYFDDVHGTPAYKRHLTYYFSEQIRAELARPAK
                     PAQPGVRA"
     gene            9409..>11671
                     /locus_tag="VGA15_27280"
     CDS             9409..>11671
                     /locus_tag="VGA15_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9591434.1"
                     /translation="MNYTVNGEKFSAEPQPGQCLRTFLRDRGVFGVKKGCDAGDCGAC
                     TVWLDGTPVHSCLMPAFRAAGREVITIEGLARGGGLHPVQQAFLDAQAFQCGFCSAGM
                     IMTVASLSDEQRADLPHALKGNLCRCTGYRAIKDAIHGVGSAEEDVAGEACGASLANP
                     FGETIVTGQARYTMDLTVEGLAHLKVLRSPHAHARIIRIDRGAAMAVPGVIEVFTWED
                     VPRRLYSTATHEDHLVDPDDSYILDNVVRFVGQRVAAVVAETEAAAEAGCRALEIEYE
                     ILPAVFDPVAAMEPDAPILHQKGGEAKGNIYVDIHGEVGSVEQGFKEADAVHEMTYST
                     SRVQHVHLETHGSIAWKDEHGRLHVRTSSQAPFVAQQKLCHLFGLFPRNVHVFTERVG
                     GGFGGKQEMISEDLCVLATRKIGRPVKWEFTREEQFIGATTRHQMTTHVKLGAKRDGT
                     LTAIEIHVVSNTGAYGGHAGETLAAALGSPLTAYRCANKKADGYAVYTNMVPAGGFRG
                     YGSSQTTFAIECAVDEIAQLIGMDPFAIRRKNMVRPGDWMESVWKDPSDVDFGSYGLD
                     QCLDMVEKELAGGRGSSKPDGSEWAEGTGIALTMLDCGPPTEHRSGALMTLLPDGRYH
                     LAVGSTEMGNGTMTAHRQIAACHLEARADQIDIINADTDLTPYDSGTFASTGTVVAGQ
                     AVGLTARALRNNILDYAARKTGTDPAAWRLEHDSVVSGNNRMSLVDLHAAGIKAGHRF
                     EAKRKAYLSPRTVA"
BASE COUNT         2235 a         3618 c         3711 g         2107 t
ORIGIN      
        1 aggacgacat cccgcacacg gtcgcctcct cgacgcggat gttcaagtcg aaagccctcg
       61 acaccgacga cagctactcc ttcaccttca atgagcccgg gagctacggg tatttctgct
      121 cgctgcaccc gcacatgacg ggaaagatcg tggtcgagcc ggcgaatcac gccgcgcagt
      181 gaacgcgacg cgatcgaggc ccgggggcat tccacgacgc ggtttaggtc ttgtctccat
      241 tttggcccaa ggaggccatt ccacagacag ggaggaggac gtcatggcgc acaaccacga
      301 caatcacgaa tacggcgtca gccgtcggca cgccatcgaa cgcatgatct gggtcggcac
      361 cggcgttcta tggacggttt cgggcggtgt accgaagtcg ttgagcctgc tcgggcgggc
      421 cgaagcggcg accgccagcg cccagacgaa gccgacgatt ccgatcatcg tcaaagatac
      481 gacgtcgctc tactggcagg tggtgctggc cggcgcgcgc aaggccggtc aggatctcgg
      541 cgtcaacgtg gccgagctcg gtgcccaatc cgaatccgac gtcgacggcc agatcaggat
      601 tctcgagaac gccgtcgcgt cgaacccggc ggcggtcgtc atcgcgcccg cgcagtttgc
      661 cccgcttggc aagccgatcg atgaagctgc caagaaagtc aagatcatcg gcatcgattc
      721 cgctgccgat tccagagcct tgacctcgtt cttgacgacc gacaacgagc aggcgggtcg
      781 catcgctgcc gatattctcg ccgaggcgat caagaggaca tacgccgatg ccgaaggcga
      841 cgtggcgctc atcacctcgt tacccgggat cgcatcgctc gatcagcgcg ccaagggctt
      901 caaggagcca atcgccgcga aatatggtgc cttggatatc gtcgccgaga aggtcggcga
      961 cggcaaggcg gcgaccggct ccaagatcat gatggacctc atcgccgagc atccggagct
     1021 gcgcggagtg ttcgcctcga acctggtcat ggcgcaaggc gcggccctgg cggtggccga
     1081 gaacgggacg aacaagaccg gcgacaagat ctatctcgtc ggcttcggct tggatgacga
     1141 cctcgtcaga tttctgcaga acggcacgat cgccgcgctc gtcgtgcaga acccgttccg
     1201 catgggttat gacggcgtca agatcgcgct cgccgcctcg aggggcgaac aagtgccggc
     1261 caacgtcgat accggcgcca accccatcac gaaggccaac atgaactccg cgcgttcgca
     1321 ggaactgctc aacccgagga tcaagtgatc tgacacgatg cactgtggcc caggagagag
     1381 atcgagtggt ggccgtcgaa aaccgattga ctgcgcacga caattgcttg gccgtttgcc
     1441 aagcggctcg ccgcgtcacc caattttatg cgcgcgctgt tgagctatcg ggtcgcagtc
     1501 gggccatcgc ggccacgata gggggacgtg cagccgaaga tcagtcgcgc cgggtggcat
     1561 ttccagcacc ctacgcacag agctgaatcg cccagcattg ctgcggtacg cccaaagcat
     1621 cgaatttcga ctcagcgccg ccggcccaat ggctccagtt cccaattaga ctcgctattt
     1681 tggccataat ggcgcaagct ttgctgtgcg cgacatgctg cgcgggacgc cacgatcacc
     1741 ttcggcaacc ccctgagcta ccccgcggtc cggctagatt atcaaaggca ccacagagga
     1801 gagcaccccc aatgaaacgg gcatcagcct tgctattctt cacttccgcc atcgtaagct
     1861 ccgcctcgct cgcatatgcg cagcacgcca ctgtggatga ccaggaactc atgacgaagc
     1921 tgaggcaggc cgcgccagca cacgttctgg aacaggcaac ggtgatgaac acgggccatg
     1981 acggtaagat gaaggtgatc caagagggaa acaatggatg gacgtgcatg gaccctggag
     2041 gagcgccaat gtgcgccgac aaggcggcca tggagtgggt agaagcctgg cagagcaaag
     2101 gaccggcacc acagagactc ggtttcattt acatgctgag cggggacaac ggggcaagca
     2161 ataccgatcc ctatgccacc aaagagacct cggacaataa ctggatcaag acgggctcac
     2221 atgtgatgat tgtgggtgcc gaggccaaga gcatgatgca ggggtacccg agagacgcca
     2281 aagccgatcc caacaagcca tacgtcatgt ggccgggaac tccgtatgaa catctgatgt
     2341 tgccaaccaa atagcccttt cgaagaggga aatgatggag tggcgcgacc aagtggcggt
     2401 cgtgacgggc tccgcccgtg gcatcggccg agcaacggcc cggcaattgg cgcaacgcgg
     2461 cgccgccgta tgcgtgaact atgctgcgca tgcggacgcg gccgaggccc tgatcgccga
     2521 gattgcggcc acgggcgggc gggcgatcgc agcggcggcg gacgtcgcgg aggccgccgc
     2581 ggtcgagacg atggtcgccc gaaccgaagc agagctgggt ccggtgacca tccttgtgaa
     2641 caacgccggc gtgcagtggc agggcacgct ggacacctac gacccggaga tggtcgcgcg
     2701 gatgcggggg gtgaacgtcg atggcgtgat ccatgccacg cgcgcggtga tggggggcat
     2761 gcgggcgagg cgctacggcc gcatcgtcaa cgtgagctcg gtcgcggcga tcggcaccgc
     2821 gctgcctggc aatgcgttct acgcagcaac caaggccgag gtggtgagtc tgacgcggcg
     2881 ctttgccatg gagctgggtc cgcacggcat caccgtgaac gccgtggcgc caggcttcgt
     2941 ccgcaccgac atgacgcagc gcggccgcgg tgcaacggat tggcagggga cgcaggagcg
     3001 cctcgccgca cgagcgatga tgggccgcat cggtgaaccg gaagacatcg ccaatgcggt
     3061 ggtgttcctg gtctcgccgg agtcgggctg gatcacggca cagatgctga cggtggacgg
     3121 cgggcggatg gactacatcg gacacggtta gaatcgagcg ttgggttccg acgtcacgct
     3181 cgacctgttt tttgccggct gcaacaggag ctctgcgcca tgccgatgac tcgaatcctc
     3241 gccgcgatat ttgtcttgtc gttcgtgctc tgcaccggcc cttcggcgtt acgagctgcc
     3301 gactgcgagg acacggttgc ccggcatatg gtcggccagg ctctgctggc cgcgcacttc
     3361 gtcgccctcg ccgaaaaggc gggcatgacg cccggcgaga tcaatgcgat cctgaaaagc
     3421 gttgctgaaa aatctgtcat gcaggaattc tggatcaccg attccgcggg ccacgcttac
     3481 ttgaccaaca ccgggatcga cttcacgttc agcccggatt cgacaaaggc gccccaggcg
     3541 tccgcattct ggccgctgat caacggcagt aaggacattg tgatccaggg cgcgcgcaag
     3601 cgcgaaatcg atgaccaaat tttcaaatac gtgggcgtcg gcggcgtcga caaagcccgg
     3661 atcgtccagg tgggtgtcgg cgcaggaaac ctgtgcaaat agcggcgcac tcggatcggg
     3721 ttcacggccg cctgttaaat caatgcgttc gcgagtagac gtgctagcgc aactatccgg
     3781 gaggcaagcc atgcgaggcc aatcgctgct caaacgctac gcttatgcga cggcaacgtt
     3841 cattgcgctg acgggtttgg gcacaacgac gaaatcacaa gatctgccat cctacatggc
     3901 gccgatttcg ggtcgcacgg tctccagtcc cgccgacacc gcggtcaaaa acgtgttggc
     3961 gctgaacacc ggcatgttcg agctctatga cgacgctgca aaaatcttcc aggggaacat
     4021 tcttgccaag catcccgtca ttctcggcct gttctcgggg gcgggcggta ggtttattct
     4081 ttatcggccc ggcatggcgc cgctcgaggc gccgtcggtg cccatcgtct atcaattgct
     4141 caaatccgtc gaccacagca ccatggcgct cagccaggta gtggggccgt atctcgataa
     4201 tcccgccgac cagtcgtggc gcggcccgat gctcgcgtac cgcgcccgga tgcagtccgc
     4261 cctcgctggc ctggacggga cgtcgatgcc accggagtgg cgggacaacg atcgcattat
     4321 tctgcagaac aacattgcat tcatggacga ttgtttggcc aacggtgcca tctccttcgc
     4381 cgcgctggag gcgttcgcca agaaacaggc gccgtacctg aaggaagaca tcgcctgggc
     4441 ggcgcagatt caggtcgccc attggatgaa ggtgctggcg gattggaaga agatgctcgg
     4501 cggtgattgg gataagacct acgcggccag caattcgatc tatgccacgc gacaaaacaa
     4561 cgtattgttc agcgtgttgg cgcaattttt cgggtcggag gccgtcaacg accggctcgt
     4621 cctgatcgag accatctcct tcaccatgac gcccaacgac atgatggagg ctctcgcccg
     4681 catcatcgcc gaccgctcgg atggagcgct attctttggc aattactaca tgatggacta
     4741 tgaattaatg ggaggcgatg cgcgcgccgc gatcgtcgct gaaagcacca aactcggaac
     4801 caagccgttc ctaccgccac tggtgccgtt cgggtcaaag cagtggccga tgcggatcac
     4861 gccgggtccg ggacccggtt ccattgccga gcttccgtga ggcgaccgcc cgcgtcggtc
     4921 gagcaccctt catgcactca gatggctaaa accacgcctc tgcgttggcg tggcggagcc
     4981 gctcgatggg gaggacgcca tgatcacgcg acaccgcttg ctcgagaccg gcgtcggcac
     5041 ggcgctcgtc ggacccgggc tgccgtggct cggcgctgcg gcgttggccc aaggcaggcc
     5101 gaagctttcg ccgctgttgc ccgtgggcac gcgctcggag gcgattttcg acgcgctgcg
     5161 gggcaagaag ccgctaatca gcgcgcaccc gggttcgatc gcgtgcaagc caattgcggc
     5221 gcctgccacg gcctcgattt cgtgccgatg actccccgcc tctgaacgcg tccgcctggc
     5281 aggtcgaggt cgacaagatg atccatgcct tcggggcgcc gatcgacgat gcggacgcca
     5341 agattatcac tgagtatctg atcaagaatt acggtggttg aggcgccgac cgtctcaaga
     5401 ttgccacgag cagccttgcg ggctactgtc ttgctcgaaa cttgggatca tctggagaat
     5461 cttggctgag gtctgtgtgg ggagttgccg ccatcgtgag catgaccgaa cgagagtacg
     5521 ggccgaagaa caataggaaa cctttcggct ttgtcattaa catgctgttg gcgcttgttt
     5581 gcctgctgat ttcggcaggc ccggcagtca gtgaaaatac cggcgactgg ataaccgggt
     5641 tgccgcgaga acccattcat gtggcagcgt ggccgggagg aaggaagatc gcggtctgtt
     5701 tcattttcta cgtggaaata tggggttttg ggcacggccc gaattttcga tcggatatgg
     5761 ttgcgcggga ccccgatgtc gtcgacgaag cgtttcgtca atatgccgta gattggggca
     5821 ttcctcgggt tggaaggctg ttcgatgagc aagggatgcc cttgagcatt gccttgaatg
     5881 cgctttttcc agagacgcgc cccgatctct ggaagcaatt tcgttcgctg gtcccaaaag
     5941 cccctatcat cgcgcacggc atcaacaact cgaccgacct gctcccgctt gggcgcggcc
     6001 tcgacgaaca gagagcatac attcaccgca ctctcgacct gatcgagaag tatacgggcg
     6061 tgaggtcacg gggctggacg agtcctagcg tataccccaa cggcgataca ttcagggcca
     6121 ccgccgccga aggcatcgcc tattcgctcg acggcatgga ctctgatatc ctctctcgcc
     6181 tcaccaccca gtcgggaccg ctggtcttaa ttccctatcc ggcagtcaca gtggatatgg
     6241 ggcaatactt gcagcgcttg aaacaaccag ctgatatgga gcggttgtgg atcgattatg
     6301 taaccgaact ggccagggag gccgaggcgc accccgaccg tgaggccact gtcgttgcga
     6361 tcggcatcca tcccttcgtg gtggggacgc ccgatggcgc tgcggcattg cggcgtgttc
     6421 tcgagaattt caagaatcag aagcttgtct gggtgacgga cgtcgaagcg gtactggggg
     6481 ttgcgggcga gcagcggtag cggcgatggc ggaatcttgt cggagagccc acccatgact
     6541 tgccgcgatc attgcgcttc gagtaccgca atctcccttc ccagccgccg cgccgtcgtt
     6601 gcggcgatgg gttccgccgt cgtgttgtcc gcggcccctt tgatcgccgc gaccgacgtg
     6661 gcgaccgaag acgaacgctt catgcgcttg gcgatcgaag aggcttggca agccgatttt
     6721 ccgtttgggg cagtcatcgt gcgtgacggg ctggtgctcg cgcgcgggcg taatctcggg
     6781 cggacgaacg gcgacccgac cgcgcatggc gaaatggtcg cgatccgccg cttcctggca
     6841 gatcacggca gcgctgcgct gcagggtagc acgctctaca cttccggcga gccgtgcgcc
     6901 atgtgtatgg gagcgatctt gtggtgccgc atcggtcggc tcgtcttcgc cgcgtccctg
     6961 cagcagctag ccaccaagat cgatcagatc atgatttcga gtgtcgatct cgccgcgaag
     7021 acccctttcg tcccgattgc catcacgggt ggcgtcttgg cggacgacgc tatgcagctt
     7081 tttgccaaat gagtattggc cacgcgatgt cacagtcccg acaccaaagt aagatcgcca
     7141 gatcgggcgt ccaatcagaa tttgattgtc gagcggtccc ggcgtaggat gtccgtgatt
     7201 gcagaactcg cggcactgga acgtgtgtcg cattgagcgc ccggttcgcg gggtgccgtc
     7261 ggcataaagg gggtagacgg cacttctacc tgtgctttgc gtgaacgcac catgcggcaa
     7321 cgagattcct tgccggtcat ccgatcttca attctgggag actttcgtga aacatgcctc
     7381 gaccttgatc ggcgccgctt gcgcgctgct cggctcactt gccatcaccg gacccggctc
     7441 gcaggcgcaa ggcgccgagg tgccgatcta cctcaagtcg atcgtcggca cgcaagccgc
     7501 gagtccggcc gaggtcggca cgaagaacat cctccagctc aacacctcga tgttcgagct
     7561 gtatgacggt tcttccaaga tcttccagag gaactttctg gcgaaacatc cggtcatcct
     7621 cggcttgttc tcgggagccg gcgggcgatt catcctgtac cggcccggca tcgcaccgat
     7681 cgaggcaccg tccgtaccca tcgtctatca gttgatgaag tccgtcggcc acagcaccat
     7741 ggcgctggcc gaggtggtga tgccttacct cgatagcccc aatgaccata gctggcgcgg
     7801 cgcgctggcg gcgtaccgga gccggatgca gtccgcgctc gacggcctcg aggcgacccc
     7861 gatgcgggac gactggcggc caaccaaccg tgccatactg cagaacaata ttgcgttcat
     7921 ggatgagtgc ctggcgaaga acgcgatctc cttggaggcc ctgcaagcct ttgccaagaa
     7981 gcaggcaccg cttttgaaag agaatatcgc ctgggcggcg gagacccagg tgggccattg
     8041 gatgaacgtg atcgccgaat ggaagctgat gctcggcgcc gactgggaca agaccttcgc
     8101 ggccagcaac accatctacg tcgcgcggca gaataacgtt ctgttcagcg tgctcgccca
     8161 gtatttcgga cctgacgcca tcaacgaccg tctgctgctg gtcgagacca tctcgttcac
     8221 gacgacgccg gacgaaatgt tggaggcgct gacccgcgtc gtcgccgacc gtcccgtggg
     8281 ccagattttc tttggcaact atcatttgat ggacttcgag ctgatgggcg gcgatgggcg
     8341 ccgggcgatc gtcgacgaag acgccaagcg caagatcgac cccaccttgc cgccgctggt
     8401 gccgtggggc tcgcatcaat ggccggcgct ggtcacgccc gggcccggtc ccaaaacgct
     8461 cgcggatctt ccttgaagtc aagtgcagcg cgcacaccgc gcgcgctcta cccggcccaa
     8521 ccagctcggc tgtcagccgc ccccggaggc gaaccgcatg aacctgaatt cggtcgtcga
     8581 ggtcaaacgt ccggcctcgg ccgacgagat caggcagtgg cgcgatggat atgcctggct
     8641 tgcaggcggc acgtggctgt tctcggagcc gcaggacgcg accgacacgc tgatcgatct
     8701 cgatcagctg cagtggccgg ccctcgaggc atcatccgcc gggctcgaca ttgccgcgac
     8761 ctgccgcatt gccgagctct atcgcttcga aggaccggcc gagtggatcg ccacgccgtt
     8821 gctgcgggag tgctgcaatt gtttcctggc gtcgttcaag atctggaatg cggcgacgat
     8881 cggcggcaac atctgcatgt cattgccggc cggccccatg atttcgctgg ctgccgcctt
     8941 ggaggcgacg ctgacactgt ggccgcgcga cgccgcccca cgggaagtcc cggctgtcga
     9001 ttttgtcacg ggcaaccaca ccaacgtgtt gcaacggggc gaattgctcc gcaacatcca
     9061 tctgccggca cgcgcccttt ccgggcgctt tgcgttgcgt cgcgcttcgc tcacccagct
     9121 tggacgatcg gccgctctgg tgatcggaac gcgtggcgta cattccgacg acgatctcct
     9181 gatcacgatc acggcgacga cgccgcgccc gctgcagctg cgtttcgacc gcttcccctc
     9241 ggccaatgac ttgcgtcaag caatcgatgc gcgaattccc gccgattcct acttcgatga
     9301 tgtgcacggg acgccagcct acaagcggca tctcacctat tatttttccg agcaaatccg
     9361 cgccgagctg gcgcggccag ccaaaccggc gcagcccggg gtccgggcat gaactacacc
     9421 gtcaatggcg agaagttctc cgctgaaccg cagccgggtc agtgcctgcg caccttcctg
     9481 cgcgaccgcg gtgtgtttgg cgtcaagaag gggtgcgacg ccggcgattg cggcgcctgc
     9541 acggtctggc tcgacgggac gccggtgcat tcctgcctga tgccggcctt ccgcgccgcc
     9601 ggacgcgagg tcatcacgat cgagggactg gcgcgcggcg gcggactgca tccggtgcag
     9661 caggcctttc tcgacgcgca ggcgtttcag tgcggatttt gctcggccgg catgatcatg
     9721 acggttgcct cgctcagtga tgagcaacga gcggatctgc ctcatgcgct gaagggcaac
     9781 ctgtgccgct gcacgggcta tcgcgcgatc aaggacgcaa tccacggcgt cggctccgcc
     9841 gaggaggatg tggccggcga agcctgcggc gcgagcctgg cgaacccatt cggcgagacg
     9901 atcgtcaccg ggcaagctcg ctacaccatg gatctgacgg tggaggggct ggcacacctc
     9961 aaggtgctgc ggtcgcccca tgcccatgcg cggatcatcc ggatcgaccg cggtgcggcg
    10021 atggcggtgc ccggtgtcat cgaggtcttc acctgggagg acgtcccgcg gcggctctac
    10081 agcacggcta cccacgagga ccacctcgtc gatcccgacg acagctacat tctcgacaat
    10141 gtggtgcgct ttgtcggcca gcgggtcgcc gcggtcgtcg ccgagacgga ggccgctgcc
    10201 gaagccggct gccgagcgct cgagatcgag tacgaaatcc tgcccgcggt gttcgatccg
    10261 gtcgccgcca tggagccgga tgcgccgatc ctccaccaga aaggcggcga ggccaaaggc
    10321 aacatctatg tggatattca cggcgaggtc ggaagcgtcg agcagggctt caaggaagcc
    10381 gatgccgtcc acgagatgac ctattcgacg tcgagggtgc agcacgtcca cctcgagacg
    10441 cacggatcga tcgcctggaa ggacgagcac ggacggcttc acgtgcgcac cagctcacag
    10501 gcgccgttcg ttgcccagca gaagctttgc catctcttcg ggctgtttcc gcggaacgtg
    10561 cacgtcttca ccgagcgtgt aggcggcggg ttcggcggca agcaggagat gatctcggaa
    10621 gatctgtgcg tgctggcgac gcggaagatc gggcggccgg tgaaatggga gttcacccgc
    10681 gaggagcagt tcatcggcgc gaccacgcgc caccagatga ccacgcatgt gaagctcggc
    10741 gccaagcggg atgggacgct gaccgcgatc gagatccacg tcgtctccaa tacgggcgcc
    10801 tatgggggac acgcgggcga gacgctcgcg gccgcgctcg gcagcccgct caccgcctat
    10861 cgttgcgcca acaagaaggc ggacggctac gcggtctata ccaacatggt cccggcaggg
    10921 ggctttcgcg gctacggttc gtcacagacg acctttgcga tcgaatgcgc ggtcgacgag
    10981 atcgcacagc tgatcggcat ggatccgttc gcgattcgcc gcaagaacat ggttcggccc
    11041 ggcgactgga tggaatccgt ttggaaggac ccgtccgacg tcgacttcgg cagctacggg
    11101 ctcgaccagt gcctcgacat ggttgagaag gagctggccg gcggccgtgg atcgtcaaag
    11161 ccggatggca gcgagtgggc cgagggcacc ggcatagcgc tcacgatgct ggattgcggg
    11221 ccgccgaccg agcatcgctc gggcgcgctg atgacgctgc tgcccgatgg gcgctaccac
    11281 ctcgccgtcg gctcgaccga gatgggcaac ggaaccatga ccgcgcatcg ccagatcgcc
    11341 gcctgccatc tcgaggcgcg cgccgatcag atcgacatca tcaatgccga tactgatctg
    11401 acgccttacg acagcggtac cttcgctagc accggaacgg tcgtggccgg ccaggcggtg
    11461 ggcctgacgg ctcgagcgtt gcgcaacaac attctcgact atgcggcgcg caagacgggc
    11521 accgatccgg ccgcctggcg cttggagcac gacagcgtcg tcagcggcaa caaccgcatg
    11581 tcgctcgtcg atctccatgc cgcgggaatc aaagccggcc atcgcttcga ggccaagcgc
    11641 aaggcgtacc tgtcgccgcg tacggtcgcc t
//
LOCUS       DASWGM010001010         3181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2739700,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001010 DASWGM010000000
VERSION     DASWGM010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2739700"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VGA15_27285"
     CDS             complement(<1..530)
                     /locus_tag="VGA15_27285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591435.1"
                     /translation="MNVSGSRLSNRPPAFRTGLAVTLFLSGVVGATGAFADDDDINLR
                     PGNLLLSRSVYDNNPANVVAGTTLLPPNCVAPNCVTATDSGAYPFVFSNDAVDASFGI
                     TSKIVLDQFEPSGKFVNSLEVELPDRSERRGQPTRDQVVTSFPSKSEIALNLSTDGRV
                     VTFMGYLAPINALDVS"
     gene            complement(1017..1454)
                     /locus_tag="VGA15_27290"
     CDS             complement(1017..1454)
                     /locus_tag="VGA15_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEX9591436.1"
                     /translation="MTTSFRTTFALLAVFAAAGFTPVAAQTPSPAGAKAYFINLKDGD
                     TVSSPFLVQFGLSGMGVAPAGVEKPNTGHHHLLIDAATLTEDQMKEPLPMDDSHKHFG
                     GGQTEAMVTLPEGKHTLQLLLGDWTHIPHVPPVMSEPITITVR"
     gene            complement(1681..>3181)
                     /locus_tag="VGA15_27295"
     CDS             complement(1681..>3181)
                     /locus_tag="VGA15_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX9591437.1"
                     /translation="SQTYSRCRRFASFLASRNIADGDTVAAMLPNIPAMNEVHFAVPM
                     AGAVLNALNIRLDAASIAFQLDHGGAKIILVDPEFAGVISEALTLMTGLKPFVIDVGD
                     AAFAGGKRIGEIEYEDALSQGDESFAARPPQDEWDAIALSYTSGTTGNPKGVVTHHRG
                     AYLNAVSNILAANLGQHPVYLWTLPMFHCNGWCFPWTIAAAAGVNVCLRKVDPSKIFE
                     LIAKHGVTHMCGAPIVYNTLINAPDAPKRATARPVVGLIAGAAPPVAVLEGAESIGIK
                     LTHVYGLTEVYGPASVCAEQPGWDDLAADQRAQLKRRQGVPYPLQEAVTVIDPETMSE
                     VPRDGETIGEVMFRGNIVMKGYLKNEKATREAFAGGWFHTGDLGVLDETGYVIIKDRS
                     KDIIISGGENISSIEVEDVLHKHPAVLFAAVVAKPDLKWGEVPCAFVELKDNARATEA
                     EIIAFCRTHMPGFKTPKAVVFGPIPKTSTGKIQKFMLRDEVKSAKAISA"
BASE COUNT          609 a          940 c          986 g          646 t
ORIGIN      
        1 ttcgagacat cgagcgcatt gatgggcgca agataaccca tgaacgtcac gacccggcca
       61 tcggtcgaca ggttgagcgc gatctccgat ttcgacggaa agctggtgac cacctgatcc
      121 cttgtcggtt ggccccggcg ctctgaacgg tcgggcaatt cgacctcgag cgagttgacg
      181 aacttgccgg acggctcgaa ctgatccagc acgatctttg aggtgatgcc aaaactggcg
      241 tcgacggcat cattgctaaa gacaaatgga taggcgccgc tgtcggtcgc ggtcacgcaa
      301 ttgggagcta cgcaattggg cggcagcaag gtcgtgcctg cgacgacatt cgccggattg
      361 ttgtcatata cggagcggct gagcagcaaa tttccgggtc ggagattaat gtcatcgtcg
      421 tcggcaaagg ctcccgtggc gcccaccacg cccgacaaaa aaagcgtgac ggccagaccc
      481 gtgcgaaagg ccggcggccg gttggacaag cgcgatcctg aaacattcat tgaaaatatc
      541 ctcccagtgc ttcgcctgca ggcaacatgc tgtgcgcggc gatcgccgga gacttgcccc
      601 tgttttgtta caaacataag aacccgatgc gacagccgcg ctgtcgtcac caacatacat
      661 tccgatctgg attacgaggt gctgcgtcag ggccggccgg cgtcattccg gcctatatga
      721 cggcttgcgg ctgacgttgg cgcgtgcggg gtgacgcggg cagctgctcc gcgattgcgt
      781 tcgacataaa gctatcgcat atgactgaat tgaaccgcgc gttgatcggc cgggcaagtt
      841 tcaacgagtc gtccctgcga acgcagggac cccagcgtga gcgcaattgc gctcatcgcg
      901 gcggcgcgga ctcccgtttt cgcactggag tagaggactt tctgactttc gaggccaggg
      961 gtgatggatc cctgcgttcg cagctacgac ccgctaggag atagaccagt gcgaggctag
     1021 cgtaccgtga ttgtgatcgg ctcggacatt accggcggga catgcgggat atgggtccaa
     1081 tcgccgagca gtagctggag ggtgtgcttg ccctccggca gcgtaaccat ggcctcggtc
     1141 tggccaccgc caaaatgctt gtgactatcg tccattggaa gaggctcttt catctgatct
     1201 tcagtcaaag tggcggcatc gatcagcaga tgatggtggc cggtattcgg cttctccacg
     1261 ccagctggag ccacacccat accagataat ccgaactgca cgaggaatgg actgctcacg
     1321 gtgtcgccgt ccttcagatt gatgaagtaa gctttggctc ccgctggcga tggcgtttgc
     1381 gccgcgaccg gcgtgaaacc cgctgcagcg aaaacggcaa gcagcgcgaa tgtcgttctg
     1441 aacgaagtgg tcatctgtac ccctgtaaaa caatcaatcg gatcacgcct agctattcga
     1501 ggtctgtggg taaatttagt cgatgctgag cgccgcaggc atactagcgc ggtattgcca
     1561 gcgcgagcgc aatcgcgaag tgataaattc ttgttgagtt gcctttttta caggcatgaa
     1621 tccgcagcac ctggcgcgcg cggttttgac ttcgcgtcta tgagttcagg ccccaagccc
     1681 tcaggccgaa atcgccttcg ccgacttgac ctcgtcgcgc agcatgaact tctggatctt
     1741 cccggtcgag gtctttggga tcgggccgaa caccacggcc ttcggtgtct tgaatcccgg
     1801 catatgagtg cggcaaaacg cgatgatctc ggcctcggta gcgcgggcgt tgtccttcaa
     1861 ttcgacgaag gcgcagggca cttcgcccca ctttaaatcc ggctttgcca ccacggcggc
     1921 gaacagcacc gcgggatgct tgtggagcac atcctcgacc tcgatcgagg agatgttctc
     1981 gccgcccgag atgatgatgt ccttggagcg atccttgata atgacatagc cggtttcatc
     2041 gagcacgccg aggtcgccgg tgtgaaacca gccgccggca aaggcttcgc gcgtcgcttt
     2101 ctcgttcttg aggtatccct tcatcacgat gttgccgcgg aacatgactt ccccgatggt
     2161 ctcgccgtcg cgcggcacct cgctcatggt ttcgggatcg atcacggtca ccgcttcctg
     2221 cagcgggtag ggcacgccct ggcggcgctt gagttgcgcg cgttggtcgg ctgcgaggtc
     2281 gtcccagccg ggctgttccg cgcacaccga ggcggggcca tagacttcgg tcaggccgta
     2341 gacatgggtc agcttgatcc cgatgctttc ggcgccctcc agcacggcaa ccgggggggc
     2401 ggcgccggcg atcaatccaa cgaccggccg cgcggtcgcg cgtttcggcg cgtcgggggc
     2461 gttgatcagc gtattgtaga cgatcggcgc accgcacatg tgggtgacgc cgtgctttgc
     2521 gatcagctcg aagatcttgc tgggatcgac cttgcgcaga cacacgttga cgccggccgc
     2581 cgccgcgatg gtccacggaa aacaccagcc gttgcagtgg aacatcggca gcgtccagag
     2641 atagaccgga tgctggccga gattggccgc caggatgttg ctgacggcgt tgagataggc
     2701 gccgcggtgg tgggtcacga cgcccttagg gttacccgtg gtgcccgagg tatagctcag
     2761 cgcgatggcg tcccattcgt cctgcggcgg ccttgcggcg aagctttcgt cgccctgcga
     2821 cagcgcgtcc tcgtattcga tctcgccgat ccgctttcct cctgcaaaag ccgcatcgcc
     2881 gacatcgatg acgaagggtt ttaatcccgt catcagggtc agcgcctccg atatcacgcc
     2941 ggcaaactcc gggtcgacca ggatgatttt cgcgccgcca tgatcgagct ggaaggcgat
     3001 cgaggcggca tcaagccgga tgttgagcgc gttgagcact gcgccggcca tcgggaccgc
     3061 gaaatgcact tcgttcatcg ccgggatgtt cggcagcatc gccgcgaccg tatcgccgtc
     3121 ggcgatattt cggcttgcga ggaacgacgc aaaacgccgg cagcgcgaat aggtctgcga
     3181 c
//
LOCUS       DASWGM010001011         6695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1492710,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001011 DASWGM010000000
VERSION     DASWGM010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492710"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..2300)
                     /locus_tag="VGA15_27300"
     CDS             complement(156..2300)
                     /locus_tag="VGA15_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591438.1"
                     /translation="MNSRIATIRSPVESRYRSPDDIVLSSMPISNRARLSRFGDDRWD
                     MTPGIFQTRARSFSRELDFAAIDCPVERLTAKEYIYASLNERLSDGRSRLRPSATVIA
                     LRDLRRFMSFVCDRLGTFDAGMIDQELSDAYRSLLVTGTAAASQQIAKYLKPIAELRR
                     LSPHLTCGGLNFQPWGARPLYRIAGCGPKGDENRTPRIPEPVIGALLRWSLKYIDLFS
                     VDIFTARAELTALESRRAARPRRRLNDVLDRVGAWIDARRQSGRGIPVWEKPVGVGGR
                     ASALSCARGMGGEVINMRLIMLQTGVNVQTIQENDAALKLIQDAVTELGIERGGMDSV
                     LSIDPDTGLPWRGRFDPISLAEEEKHLQSAAYVLCAYLTGMRDGEVQSMRPGCVARSL
                     STDGMIERLAVRSTVYKHRGTRGDSAEWITIEPVERAVAVAERLAAQHRRLRDADDLW
                     LILDRRSSAARRGIPGIVKQINRFRAALDKRYGSIDQSALPMIDGQPWRFNTRQFRRT
                     LAWYIANRPFGVVAGKIQYKHASVAMFDGYAGSSASGFRQEVEQERALGQLDDIVAQY
                     EGHRRGEKQAGPAGSRLTAEYERVERELDSFPGRIADKGRERAMLAHLARTLHVGHLN
                     DCFFEPATALCLDKVDVKERTTPVLSRCAPDRCPNSCITIRHLPIWEASIAEADALLQ
                     DKRLPRFQREALQRDNDRKRKLIAPLKAGDAS"
     gene            complement(2297..4111)
                     /locus_tag="VGA15_27305"
     CDS             complement(2297..4111)
                     /locus_tag="VGA15_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591439.1"
                     /translation="MSGRGRRATFPDVHAHRATADLVRIVQTSDPRRFRVRVDGGGEM
                     AIDLSSWLHPAFSTAIAPLLQELIRQMGPAPVRRTVYCKVLDLRRFWRFLDASGDTIG
                     GLDDLTADLINRYEDWLEQNTSSQASLRRLISPVIALLRLAVEQTPDRFPQSLVNRLN
                     WLGRGEFASSHPRDAYSTGVTAALRAAARKQIAEAVKRILLVDKLPDRRIDIEASPVL
                     HPFYDDVIARIVKDGSTATHNPVFRRLKGALWNRKLPPVSSEELHGQLYLTRLDLIAF
                     LVLLSLETGMEIECLKSLKADCLRNPSRGYVEIEYRKRRARGAEWKRLRVRDGASSTP
                     GGMIRQALRLTERARHYLGTDALWAWWNGYELSHAKPGVRSGSAFVAHHVLLGDDGQP
                     LHLELSRLRKTQKAERYLRTQGQLDDFAIGHTVAVAARHYADIPALRQVHERTIADAL
                     QDALDAALKPKLLPPILEEAARSNPEAAGLPLAADQVTALLDGDEDVWLASCGGFHAS
                     PFGALGEPCPTPFWGCLECANAVITARKLPALIAFDTFMTGQRAAMDADTWAARFGRP
                     HRRIVEQILPAFPPQIVAEARAAAEAGLLYLPPEAHAS"
     gene            complement(4108..5673)
                     /locus_tag="VGA15_27310"
     CDS             complement(4108..5673)
                     /locus_tag="VGA15_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX9591440.1"
                     /translation="MQAKSCTPATRRAGEREGNPSHSKERPAPKALRFGERMPQLYFT
                     DLRALRRPAIVDSVSLELPFEIIATAATFGLIDGMPFILGDDGSYDHDLNRFFRACPT
                     MGLRSLNSLRAYARDIVVWLRFLAERRAGKSIWGADRDDIAAFHEARRLSSPTHRISA
                     SSWNRSVAALDKLYRWAVEEKLICKAPFTYRQVWTRALDGSTLSVSANMARESAGRNG
                     DTRFVSLDRYLQFRDVGLRGRLPDGREDPSWCGRNGERNALFAELLVTTGLRLQEGSS
                     LLAVELPSSRPQHHGQRSVAFRLASATAKGSKGRDIRLPLRLVKRLRDYIEVERSIAT
                     ARCRDRQSWMRLDDPIIVRGHDRQSLRIGEMNPPDRVRFDGIKPGERFRLLHEDTMEP
                     LALWLTEGGMPMPMAAWEAVFRRASARCRSFGIDIDVTPHMLRHGFAVHMLTLLLREQ
                     IGWMLEERVGRMSPAYRRIIGDPLLKLQRLMGHSRIESTYIYLDHLDDSQALIDAAVE
                     QWGLDVASVRDAA"
     gene            complement(5824..6472)
                     /locus_tag="VGA15_27315"
                     /pseudo
     CDS             complement(5824..6472)
                     /locus_tag="VGA15_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563595.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
BASE COUNT         1299 a         2097 c         2070 g         1229 t
ORIGIN      
        1 ctcagtcgca cggttggccg tggcgcggct gacgccggcc tcgatcgcca ggttgacggc
       61 ggtcatgcgt ccgtcggtct tgagtgcatg tccggcaagg agccgttcca tcgcatcttg
      121 caacgcctgc ttggttgccg cgctgacggg cgcgatcatg aggcgtcccc cgctttgagc
      181 ggtgcgatca gcttgcgctt gcgatcattg tcacgctgaa gagcttcgcg ctggaagcgc
      241 ggcaagcgtt tgtcttgcag gagagcatcc gcttccgcga tggatgcttc ccagatcggc
      301 agatgccgga tggtgataca ggagttcggg catcggtctg gcgcgcatcg cgacaggacc
      361 ggcgttgtcc gttccttcac atcgaccttg tcaagacaca gcgccgttgc cggttcgaag
      421 aagcaatcgt tcagatgacc gacatggagc gtgcgcgcca gatgcgcgag catcgcccgc
      481 tcccgccctt tgtcggcgat ccggccgggg aatgagtcaa gctctcgctc tacgcgctcg
      541 tattcggcgg taagccggga gccggcaggg ccagcctgct tctcgccacg acgatggcct
      601 tcgtattgcg cgacgatgtc atcgagttgg ccgagcgcgc gttcctgctc gacctcctga
      661 cggaagccgg atgcagatga gcccgcgtag ccgtcgaaca tagcgaccga ggcgtgctta
      721 tactggatct tgccggcaac cacgccgaag ggccgattgg cgatatacca ggcaagcgtg
      781 cggcggaact gccgggtatt aaatcgccat ggctgcccgt cgatcatcgg aagcgcggac
      841 tggtcgatgg agccataccg cttgtcgagc gccgcgcgga accgattgat ctgcttcaca
      901 atgccaggaa tgcctctgcg agccgcgctg ctgcgccgat caagaatgag ccagagatca
      961 tctgcatcgc gtagcctgcg atgctgcgca gcgaggcgct cggcgacggc aacagcacgt
     1021 tctaccggtt cgatcgtaat ccattctgca gaatcgcctc gcgttccccg atgtttgtag
     1081 accgtgctgc gtaccgccag acgctcaatc atcccgtcgg tgctgagcga gcgcgccaca
     1141 caacctggtc gcatggactg cacctcgccg tcacgcattc cggtcagata ggcgcacagc
     1201 acataggccg ccgactgaag atgcttttct tcctcggcca gactgatcgg atcgaagcga
     1261 ccgcgccatg gcagccctgt atcggggtca atcgacagga cggagtccat tccgccgcgt
     1321 tcgatgccga gttcggtaac ggcgtcctgg atcagcttga gcgcggcgtc gttctcctgg
     1381 attgtctgga cgttcacccc ggtttgcaac atgatgaggc gcatgttgat tacttcgcca
     1441 cccatgcctc tcgcgcacga cagcgcagag gcgcggccgc ccacaccaac cggcttctcc
     1501 catacgggga tgccacgtcc ggactgacgg cgtgcatcga tccacgcgcc tacgcgatca
     1561 agaacatcgt tcagacgacg gcggggtcgt gcagctcgcc ggctttccag cgccgtcagt
     1621 tccgcgcgcg ctgtaaagat atcgaccgag aacagatcga tgtatttgag cgaccaacgc
     1681 agcagtgccc cgatcaccgg ctctggaatg cggggcgtcc ggttctcgtc gccctttggt
     1741 ccgcaaccgg cgatacggta aaggggccgt gcgccccagg gctggaagtt cagcccgcca
     1801 caggtcagat gtggcgaaag acgacgtaat tcagcgattg gctttaggta ttttgcaatt
     1861 tgctgagagg cggcggccgt gccggtgacg agtaacgatc gataggcatc actgagttcc
     1921 tgatcgatca tgcccgcatc gaatgtgccc aacctgtcgc agacgaagct catgaagcgc
     1981 cgcagatcac gcagcgcgat tacggttgcg gaaggccgca aacgggaacg tccatcggac
     2041 aagcgttcgt tcaggctcgc ataaatatat tccttggctg tgaggcgctc gaccgggcag
     2101 tcgatggcgg cgaagtcgag ttcacgcgag aagctgcgcg cacgggtctg gaagattccc
     2161 ggcgtcatat cccagcgatc atcgccgaac cgtgacaagc gggctcgatt gctgatcggc
     2221 atggatgaca gaacaatgtc gtcgggagag cgataccggg attccaccgg cgagcggatc
     2281 gttgcaatgc gcgaattcac gaggcatgcg cctccggcgg cagataaaga agaccggcct
     2341 ctgccgccgc gcgtgcttcg gcgacgatct gcggcgggaa tgccggcaat atctgttcga
     2401 cgatgcgccg atgcgggcga ccgaacctgg ccgcccaggt atccgcatcc atagctgcgc
     2461 gttggccggt catgaaggtg tcgaaggcga tcaatgcggg cagcttgcgg gccgtaatca
     2521 ccgcgttcgc gcattcaagg cacccccaga atggcgtcgg acaaggttcg cccaacgccc
     2581 caaacgggct ggcatggaag ccgccgcaac ttgccaacca cacatcctca tcgccatcga
     2641 gcaacgccgt gacttgatcc gccgcaagcg gcagcccggc ggcctcggga ttgctgcggg
     2701 cggcttcctc cagaataggc ggcaggagct ttggcttcag cgccgcatcg agtgcgtcct
     2761 gcaaagcgtc ggcgatggtg cgttcatgaa cctggcgtaa cgccgggatg tcggcgtaat
     2821 gccgcgccgc gacggcaacc gtgtgcccga tggcgaagtc gtcgagttgc ccttgggtgc
     2881 gcagatagcg ctccgccttc tgggttttgc gcaaccgcga cagttcgaga tgaagcggct
     2941 ggccgtcatc cccgagcaac acatggtgcg cgacgaacgc gctgccgctt ctgacgccag
     3001 gctttgcatg gctcagttcg tagccattcc accaggccca caatgcgtcc gtgcccagat
     3061 agtgccgtgc tcgttcggtc aatcgaagcg cctgtcggat cattccaccc ggcgtcgaac
     3121 tagcgccgtc gcgcacacgc agtcgcttcc attctgcgcc gcgcgcgcgt cgtttccggt
     3181 attcgatctc gacataaccg cgcgacgggt tgcgcaggca atcggccttg agactcttca
     3241 ggcattcgat ctccatgcct gtttccagcg acagcagcac caggaaggcg atgagatcga
     3301 gccgcgtcag atagagctgc ccgtgcagtt cctccgacga caccggcggc agctttcggt
     3361 tccacagcgc acccttgagc cggcggaaca ccggattgtg cgtggctgtc gaaccgtcct
     3421 tgacgattct ggcgatgacg tcatcgtaaa agggatgcag gactggcgaa gcctcgatgt
     3481 cgatcctgcg gtcgggcagc ttatccacga gcaggatgcg ctttaccgct tccgcaatct
     3541 gcttacgtgc ggccgcacgt aaggccgccg taacaccggt gctgtaggcg tcgcgcggat
     3601 ggctgctggc aaactcgccc cgaccgagcc agtttagccg gttcaccagt gactgcggga
     3661 accggtccgg cgtctgttcg accgcaagac gcaggagagc gatcacaggg ctgatcagac
     3721 ggcgaagaga tgcttggctc gacgtgttct gctccagcca atcctcataa cgattgatca
     3781 gatcggctgt cagatcgtca aggccgccga tcgtgtcgcc ggaggcatcg aggaaccgcc
     3841 agaaccggcg cagatcgagc accttgcagt agacggtgcg cctgactggc gcgggaccca
     3901 tctggcggat gagctcctgc aagagcggag cgatcgctgt cgagaacgct ggatgcagcc
     3961 acgacgacag atctatcgcc atctcgccgc cgccatcgac ccgaacgcgg aagcgtcgtg
     4021 gatcggacgt ttgaacaatc ctgaccagat cagcagtggc acgatgcgca tggacatcgg
     4081 ggaaggtcgc gcgacgaccg cgaccactca tgccgcgtct ctcacggagg cgacatccag
     4141 tccccattgc tcgacggcgg cgtcgatcag cgcctggctg tcgtcaaggt gatccagata
     4201 aatgtaggtg ctctcgatgc gactgtgccc catcagccgt tgcaacttga gcagcggatc
     4261 gccaatgatg cggcggtagg ctgggctcat ccgtccaacc cgctcctcca gcatccagcc
     4321 gatctgctcg cgcaacagca acgtcagcat atggaccgca aagccgtggc gaagcatatg
     4381 tggcgtcacg tcgatatcga tgccgaagct acggcatcgg gcgcttgcgc ggcggaacac
     4441 cgcctcccat gccgccatcg gcattggcat tccgccctcg gtcagccata gcgccagtgg
     4501 ctccatggtg tcttcatgca aaagtcggaa tcgctcgccg ggcttgatgc cgtcaaagcg
     4561 aacccggtcc ggaggattca tctccccgat ccggagcgac tgacgatcat gtcccctgac
     4621 aataatggga tcgtcgagcc gcatccatga ttggcgatct cgacaacgcg ccgtcgcgat
     4681 ggaccgctcg acctcaatat aatcgcgcag tcgcttcacc aaccgcagtg gaagtcggat
     4741 atcccgaccc ttgcttcctt tggccgtcgc cgatgccagc cggaatgcga ccgaacgttg
     4801 gccatgatgt tggggacgtg atgatggcaa ctccaccgca agcaggcttg aaccctcttg
     4861 caatcggagg ccggtcgtca caagaagctc cgcgaagagg gcattgcgct ctccgttacg
     4921 gccgcaccat gacgggtcct cccgcccgtc tggaagacga ccgcgcaacc ccacgtcccg
     4981 gaactgcaga tagcggtcta gtgatacgaa tcgcgtgtcg ccattccggc cagcagattc
     5041 acgcgccatg ttcgctgaca cgctcagcgt gcttccgtcc agcgcgcgtg tccacacctg
     5101 ccggtaagtg aagggcgcct tgcagatcag cttctcttcg accgcccagc gataaagctt
     5161 gtcaagcgcc gcaaccgagc ggttccacga tgacgccgag atgcgatgtg tcggagacga
     5221 caaacgacgt gcttcatgga aggcagcaat gtcgtcgcga tccgctcccc agattgattt
     5281 gcccgcgcgc cgttcggcaa ggaaacgcag ccagacgaca atatcgcggg cataggcgcg
     5341 caggctgttc agcgaacgca accccatggt cggacaggcg cggaagaacc ggttcagatc
     5401 gtgatcgtag ctgccgtcat cgccgaggat gaatggcatg ccatcgatga ggccaaaggt
     5461 cgctgccgtg gcgatgatct cgaacggaag ttcaagacta acgctatcga cgatggcggg
     5521 acggcggagt gctcttaaat ccgtaaagta aagctgcggc attcgttctc caaaacggag
     5581 cgccttcggc gcgggtcttt ctttagaatg cgaggggttc ccctcgcgct ctcccgctcg
     5641 ccgcgtggca ggggtgcagg acttcgcctg caccccataa gcagcttggg gtgtcgccgc
     5701 cgcctcaagc gttgtcctcg cttcgctgcg ggaacgcttg acccggacgt ctgatgagct
     5761 tcaacggata agccggcggc ccctctccat cgctgattct gagacagata catagagtca
     5821 ggataagaac ggattatgcg tcatggtcgc ccaatccagc acgcccggca ccttccacat
     5881 ggtgtcgccg gtgccgttgc ccgcgatgtc gatcacggca gcgacaagct cgtgatccca
     5941 gatgcggcca taatccgggc cggtgacggc gcgaagctcc acgcgaccgt catcggtttc
     6001 caatgtcttc agcaactcgg cgcggtgtga gagcaagcca tgttgcagat tgatcccggc
     6061 caacggcgcg ggaagctgcc gcatatagga tgatggcgcg gccgaactac taactgccat
     6121 cttatggagg gcgaagcggt tcatggtcgc cgacaagccc gcacaattgc ccaaaactcc
     6181 aatgtgtggg tgccaccggt tcgtcacgtc ccggcaccaa caaagccaac cgctcggcat
     6241 tatcgcgggc agcctctacc tttacggcgc ggctttccac ggtgcgggcc tgtgcgcatt
     6301 cggcgcggcg gcgtaccgca tcataaagat cggtcaggct gagataacgc tcgtcatcgg
     6361 ggcgagaaaa ccattccgac gagacgtggc caacacgctc gccgcgcgac acgtcaacgc
     6421 gaaagccact ggaaataggc cgctcgttct gggtcagggt catcaatgtc atggatcatt
     6481 ctccttatgt agctgggtta aagcgcagcg ctgcgctgca accctgcccg tcggcgagac
     6541 cgggggtgca aggtgcaagg gcggccggga cggaggggga tcacccggcc tgcacaagcc
     6601 ccggacgagg gtccggggcg cggaaggttg gggagaccgt cccggacgag cgtcagcgat
     6661 cccttgcgcc gcgcgggggg cagcgcccca agcaa
//
LOCUS       DASWGM010001012         1797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_250375,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001012 DASWGM010000000
VERSION     DASWGM010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250375"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1145
                     /locus_tag="VGA15_27320"
                     /pseudo
     CDS             118..1145
                     /locus_tag="VGA15_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142987.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(1279..1680)
                     /locus_tag="VGA15_27325"
     CDS             complement(1279..1680)
                     /locus_tag="VGA15_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX9591441.1"
                     /translation="MDRDFLIDLFADFGPVTIRRMFSGFGISADGTNFALALRGGLYL
                     RADDETIPRFEAEGSKPFQYQTRAKTVTVGSYWQLPERLYDDPEELTGWARAALAAAQ
                     RAALRKRPKAKKTVKKRVAAKRTSKKKKASS"
BASE COUNT          377 a          575 c          511 g          334 t
ORIGIN      
        1 tctttcatcc tcctccgtta acttggcggc gataatttta attattgccg cggtgagcgg
       61 gctgaactgc ccgctatttt ggggacggat ccggtgctcg aagaacacga acgcacgatg
      121 gcattcgcgg aaatagccag atcagatcgc tccggcaaac cgccgtcccc cgcaactatt
      181 aaatctggta cgtctacgcg accgggtata atccaccgct caacaagatc atcaacgaga
      241 cgctggcgcg caacggcaag ctgaccgaag ccgacctcga acagatctac gagacctatc
      301 tttcccagat caagaccacc gaccgcatcg acaaggtcgg cgcccgcgtg atcggcgaaa
      361 tcgacgacgt gatgaatctc atcaccgagg ccctcgccat gtcggccaga tacgacgaca
      421 gcctgtccgg cgccagccgg aagctcgagg ttgcccagag ccgtgaccag gtgaaaacgg
      481 tcgtcgaatc gctgatgaag tctacccgcg agatgcgcga taccaacaag gcgctggacg
      541 atcggctgac gctgtcgaaa tccgagatca gcaatctcca gcagagcctg gaggcgatca
      601 gagccgaaag cctgaccgat ccgctcaccg gtctcggcaa ccgcaaatat ttcgaccgct
      661 cgatcgatgc ggccgtgcag agtgcgctgg ccagcgacga accgctttca ctgctgatgt
      721 tcgacatcga ccacttcaaa tcgttcaacg actcctacgg ccacctcacc ggcgatcagg
      781 tgctgcggct ggtcggcatg tcgctgaggc agaccatcaa gggccaggac atcaccgccc
      841 gctacggcgg cgaggagttc gcggtggtgc tgcccaacac cgcgctgcgc caggcgctga
      901 cggtcgccga tcatatccgg cgcgccgtca tggcgaagga attgaagaaa aaatccaccg
      961 gcgagatttt ggggcgcgtc accatttcgg tcggcgtttc catgctgaag cccggcgacg
     1021 ataccgattc gctgatcgag cgcgccgacg cctgcctcta cgccgccaag cgcaacggcc
     1081 gcaatcgagt cgtctgcgaa gccgacccgg aatacgccgc cgagacccac ggccgggtcg
     1141 cctgacgtcc cgcagaccgt cattcctgct taacagctta gccgtcatcc tgaggtgcga
     1201 gctcttgcga gcctcgaagg atgaagaatg acggcgtgcg tggctatcct tcgaggccgt
     1261 cgcaagtgcg acggcacctc aggatgacgc tttctttttc tttgatgtcc tcttcgcagc
     1321 tactctcttc ttaaccgtct tcttcgcctt cgggcgcttg cgcaacgcag cgcgctgcgc
     1381 cgccgcgagg gccgccctcg cccaacccgt cagttcttcc gggtcgtcat acagccgctc
     1441 cggcaattgc cagtaggagc ccaccgtgac ggtcttggcc ctggtttgat attgaaacgg
     1501 ttttgatccc tccgcctcga accgcggaat ggtctcatcg tcggcgcgaa ggtaaaggcc
     1561 gccacgcagc gccaaggcaa agttggtacc gtctgcggaa atgccgaagc ccgagaacat
     1621 ccggcggatc gtgaccggcc cgaaatctgc aaacaagtcg atcaggaaat cgcggtccat
     1681 ggaatgccgc tcacgactct acgagtcgtc ccggcgaacg ccgggaccca tacgccgtgt
     1741 tgtctcgttt gagcagagtg gtagatgcct tctttaagaa gcgacgccag gggttat
//
LOCUS       DASWGM010001013         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2741553,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001013 DASWGM010000000
VERSION     DASWGM010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2741553"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..1535)
                     /locus_tag="VGA15_27330"
     CDS             complement(360..1535)
                     /locus_tag="VGA15_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591442.1"
                     /translation="MGLAAPALAQDKTVKIGVLNDMSSLYADIGGANSVVAIKMAVED
                     SGLPKKGWTIDVLSGDHQNKPDVGVNIARKWMDNEKVDAIADTPNSGVALAVSNLVRD
                     KNAVLLNSGAATADLTGKACTPNTVSFAYDTYMLANGTGKALTKAGGDSWFFLTSDYA
                     FGQALERDTTAVVTANGGKVLGGVKHPINTSDFSSFLLQAQASKAKVVGLANAGGDTT
                     NAIKQASEFGIVSGGQKLAALLLFVNDVHSLGLKTAQGLSFTESFYWDMNDQTRAWSK
                     RFMALSPKGTMPSMTVAGNYAAVLHYLKAMEALGGNPHDGAKVVAKMKEIPTDDPLFG
                     KGPLRIDGRRIIPAYLFEVKKPEESKYPWDYYKTIATISPEDAAKPLEASECPLVKK"
BASE COUNT          274 a          468 c          458 g          342 t
ORIGIN      
        1 gtgcattcgg attgagccgt agatattcag cgctttgcag atcgtcggct cgggtacttt
       61 tctctatcag ctgatatggc tcgcccacct cctcgagcat ccacaggcat cgcatcgctc
      121 gagatctgag gttaccatat aattgaagca tcatttggct ccaaaacacc tgacggcacc
      181 aacgctacgg cgggcagccc cacgctcgga ctaactcagt ttctcgagcc gcaatctccg
      241 accgatatct tatccaggcc gttaccgaag tcgcctgtga gtttatcaca ggataacgta
      301 gtccctcaaa tcattatcgt gctggccgga cgttgtgcgt ccggccagct gccattgcct
      361 tacttcttca ccagcggaca ctcgctggct tcgagcggct tggcggcgtc ttccggcgag
      421 atggtggcga tggtcttgta gtagtcccag ggatatttcg actcctcggg ctttttcact
      481 tcgaacagat aggccggaat gatccggcgg ccatcgatcc gtaaggggcc cttgccgaac
      541 agcgggtcgt cggtcgggat ttccttcatc ttggcgacca ccttggcgcc gtcatgcgga
      601 ttgccgccga gcgcttccat ggctttcagg tagtgcaaca cggccgcgta gtttcccgcc
      661 accgtcatcg agggcatcgt gcccttcggc gacagcgcca tgaagcgctt ggaccatgcg
      721 cgggtctggt cgttcatgtc ccagtaaaag gattccgtga acgacaagcc ctgcgcggtc
      781 ttcaggccca gcgaatgcac gtcgttgacg aacagcagca gcgctgcgag cttctggccg
      841 cccgacacaa tgccgaattc ggacgcctgc ttgatcgcgt tggtggtgtc gccgcccgca
      901 ttggcgagac cgacgacctt ggctttcgag gcctgcgcct gcagcaggaa cgaggaaaaa
      961 tccgaggtgt tgatcgggtg cttgacgccg cccagaactt tgccgccatt cgcggtgaca
     1021 accgcggtgg tgtcgcgctc cagtgcctga ccgaacgcat aatcggaggt caggaaaaac
     1081 cagctgtcgc cgccggcttt tgtcagggct ttgccagtgc cattggccag catgtaggta
     1141 tcgtaggcga aggagaccgt gttcggtgtg cacgccttgc cggtgagatc ggcggtcgcg
     1201 gcgcccgagt tcagcagcac cgcgttcttg tcgcggacga ggttgctgac cgccagcgcg
     1261 acgccggaat tcggggtatc ggcgattgca tcgaccttct cgttgtccat ccatttccgt
     1321 gcgatgttga cgccgacgtc cggtttgttc tgatggtcgc cgctgaggac gtcgatggtc
     1381 cagccttttt tcggcaggcc ggaatcctcg accgccatct tgatcgcgac caccgaattg
     1441 gcgccgccga tgtcggcata caggctcgac atgtcattca gcacgccgat cttgacggtc
     1501 ttgtcctggg ccagagcggg agcagcgagg cccaatccgg ca
//
LOCUS       DASWGM010001014         7751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1230741,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001014 DASWGM010000000
VERSION     DASWGM010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230741"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VGA15_27335"
     CDS             complement(<1..280)
                     /locus_tag="VGA15_27335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591443.1"
                     /translation="MTFRQRTIGYVAAAAVAAISTVPYAVADGIGNNGPPRTGDQAAI
                     SQSMRNKETVVKFYNEALNNKQPDEALKYVGNDYKQHNPMAEDGKEGFR"
     gene            563..1384
                     /locus_tag="VGA15_27340"
     CDS             563..1384
                     /locus_tag="VGA15_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX9591444.1"
                     /translation="MLKETIDTGTDELLCEIADRVAIITLNRPEARNALSDRLTPALR
                     RMIKLRGDDPNVGAILITGAGGAFCAGGDVKGMGGGAAKVEATFEDRVADLRTKQRTL
                     TGALVAVRKPTIAALPGPAAGAGMAIALACDIRIAAESAIMATGYSRIGLTGDYGIAW
                     LLTRLAGTARARELMFLSERLDARRCEALGLVNRVVPDGELQDTAFALAKSLAEGPPI
                     ALGYMKDNLDHAMTSGLIDSMDQEAENLVRAARTIDHKEAVRAFVDKRKPTFSGR"
     gene            2109..3332
                     /locus_tag="VGA15_27345"
     CDS             2109..3332
                     /locus_tag="VGA15_27345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591445.1"
                     /translation="MSDPKQRDLDSVGRAASWLRERSKDPDPIPLGRAKDRPDWPAQG
                     KPDETVTHLRPVEPRDWQIHKQSDVFAEAVARAMQEQGEPDLVEAPSALQNRHAFGSI
                     AARFAVAMGVSALVALVFVAAFPASQGPADEDGLASLPALKSLRSSLFPAPQRKPAPS
                     LVIRDGSGPVNERLSLGVNVKSPDPGATVTIDRMPAGARLTVGRRMSAGEWRVPAQEI
                     SDASVIPPADFVGVMNLTAELGGSDGAALVSSVVRLTWTSARPGGPVGGASASAATLP
                     SSPAAPTLQQQPQQQQPVASPGPPVVVARAEPPVREINPNEVADFVRRAQELLATGDL
                     QAARLLLLRAAQAHDGRAALLLAKSFDPIVSKRIGMADAEPDLAQARNWYQKAEEWGA
                     SEARRELDALASYPR"
     gene            complement(3390..5606)
                     /locus_tag="VGA15_27350"
     CDS             complement(3390..5606)
                     /locus_tag="VGA15_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591446.1"
                     /translation="MAGPLAAERVSAASAGDNEIRIGNTMPYSGPASAYGTIGKVISA
                     YFDKINAEGGINGRKINFISYDDAYNPAKTVELTRKLVEEDRVLLVFSSLGTAPSAAV
                     QPYLNSKKVPQLFIASGASMWDQPHEFPWTMGFQPSYQTEAHIYAQYLLENHPHGKIA
                     VLYQDDGFGKDYLKGLKDGLGGKIPIVAEAPYKVTDPTVDAQITKLKASGADIFFDVT
                     TPKFAVMVIRRTAELGWKPVHIIPTVSESVSAVMQPAGPQNAEGLLSAGYSFEGDDPA
                     AASDPVYRDWAAFMDRYVPSASKTNSLSVFGYVIAKAMVEVLVRCGDDLSRENVMRQA
                     ASLKGVQLPMLLPGIVVNTSASDHAPLEQMQMMRFSAGRWERFGSVRSGIDAGSVSDS
                     FKTIFRYGTAKHDMAAQLNANTVTLMTGSFGSTYAQIGADLSSVLDNGANLRVLPILG
                     RGSVQAVADILLLKGVDAGIVRKDTLAYLERKDFANNIRNQFVYVTKMFNEEMHVLAP
                     RSIRSLKDLDGKTVVVDLPDASTFVTSINVFERLGIKPHLLYIEPRLALEQLSRGEID
                     AIVAVEGKPVQWLSQVRDPNLHLVPVEYEKTLQEEYLPSQLSSADYPNLVSDAAPVDT
                     IAAEAVLASYNWQPTSDRYRRLSLLVDSLFTRVAQLQRPPFHPKWKEVALKAPVAGWT
                     RFQAAQEWIDRNSPSVGTTAASAAVEVPRADKPPDRDDPLYREFLEWRANRLNVRR"
     gene            5934..6059
                     /locus_tag="VGA15_27355"
                     /pseudo
     CDS             5934..6059
                     /locus_tag="VGA15_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
     gene            complement(6069..6413)
                     /locus_tag="VGA15_27360"
     CDS             complement(6069..6413)
                     /locus_tag="VGA15_27360"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX9591447.1"
                     /translation="MFGEADVEKRMTASGEFGAPLQNIINAYVYGDIWERDGLADDIR
                     SLVMLGITAASNRPAEFRVHAKGALANGCTKQQVQDVLLLVAMYCGVPAAIETNRIAS
                     EVFAESGGSSGA"
     gene            6584..7549
                     /locus_tag="VGA15_27365"
     CDS             6584..7549
                     /locus_tag="VGA15_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9591448.1"
                     /translation="MMVRLGLVACLVLPGVLSPQLAVAVDDYPNRQIKIIVPFPAGAG
                     PDQIARMLGQHLQDAFGQTVVIENRTGALGSIGAQEVARSAPDGYTLLMGTNTTQAAN
                     VAMLKNLSYDPAKDFAPIIRTITTAMVLVVKPDFPAKNLAQFIEYARAHPNVTAGYGS
                     GASQISIGQLQTRGGISVIAASYRGVPQAVTDVIAGVIDLAFADFSIAIPQLQGGTLR
                     GIGVTSPKRNDLTPDLPSLAEAMPGFEATIWYGLLAPAGTPEPIVNKLYDESEKFLTM
                     PGTREKLAGVGVIVSPMRPDEFGAFIRSEIKRWTADAKAAGIEAK"
BASE COUNT         1547 a         2323 c         2323 g         1558 t
ORIGIN      
        1 tgcgaaagcc ttccttgccg tcttccgcca tcggattgtg ctgcttgtaa tcattgccga
       61 catatttgag agcctcgtcc ggctgcttgt tattgagggc ttcgttatag aacttaacga
      121 ccgtctcttt gttgcgcata ctctgcgata tcgccgcctg gtcgcccgtg cgaggcggac
      181 cgttattgcc tattccgtcg gcaactgcgt acggaaccgt cgagatcgcg gcgactgcgg
      241 ccgcagcaac ataaccaatg gtgcgttggc gaaatgtcat gcggggttct cctgacgcgc
      301 gaggggcgct cactttcgat ccgctgacca ggatggccgc gaaggcgatc ccagcgaaga
      361 gcgcttcaat atgaccgacc atcttatatt gtcaatatca aatgcatccg gatttgcaat
      421 gcccccctct cgcaacgttt cggtccgccc cctccggccg acaacaccgg accaagcctc
      481 atggcctcgg aatatcgcat tgcaagcaac gcatcattac attacaacca tcatacttat
      541 aaccggctga aggtctggat cgatgctgaa ggaaaccatt gatacgggta ccgacgaatt
      601 gctttgcgag atcgccgaca gggttgcaat cattaccctg aatcgaccag aggcgcgaaa
      661 cgcgctttcg gatcgtctta cacccgcgct gcggcgtatg atcaagctgc gcggtgacga
      721 tccgaacgtt ggcgcgattc tgataacggg cgccggcggc gctttctgcg caggcggcga
      781 cgtcaagggc atgggcggtg gcgccgcaaa agtcgaggcc acgtttgagg atcgcgtggc
      841 tgatttgcgg accaagcagc ggacgctcac tggcgcgctt gtcgcggtcc gaaagccgac
      901 catcgcagca cttcccggac ctgccgccgg cgccggcatg gccatcgcgc tcgcctgcga
      961 catccggata gccgcggaat ctgccatcat ggcgacaggc tattcgcgca tcggattgac
     1021 cggcgattac ggcatcgcct ggctcttgac gcggctcgcc ggaaccgcac gggcacgtga
     1081 actcatgttt ctgtcggaac ggctcgacgc gagacgctgc gaggcgctgg gacttgtcaa
     1141 tcgcgtggtg ccggatggcg agttgcagga tacggcgttt gccctcgcca aatcgctggc
     1201 agaagggccg ccgattgcac ttggttacat gaaggacaat ttggaccatg cgatgacgtc
     1261 gggtctgatc gattcaatgg atcaggaggc cgagaatctg gttcgggcag cccgaacaat
     1321 cgaccacaag gaagcggttc gcgccttcgt cgacaagcgc aaaccgactt tcagcggccg
     1381 ctaatccatc ccgtgataat atctgacggc gccagaaaat ttgggcattt cctgtgatcg
     1441 cgaacttcga ccacacgact ttgctggtgc acgacatcga gatcggcatc agcgcctatc
     1501 gtcaaattct gggacgggag ccccgtccca ggtaatttcg cgattggcgt tcgttgcgat
     1561 tgatcgagac gctcgttccg cctttggtct gaacgggcga tcggatcgaa cgcacattcg
     1621 agggccgatc cgggcaagcg ttatccgctt atcctcaccc gatccaactc tgcgatattt
     1681 cttccgatgg gaggcgatcc gcgccgttaa acaaactttg gtcaatttgc ggttgcgaat
     1741 atcaatccgc tcatacttta ggccagaatg ggactcacga atgccattct cgctagcgag
     1801 catggagcgc gtcggtttgg agaagcgtca gattggaaaa ctctgatgaa ctctgcaagg
     1861 gcaaacctgc cgggaattca tgtcgcaacc ttcggctaca gcggcgtgca acgcagcatc
     1921 ggatgcgttg ctgcaatcgc atttccttgg gttgggatcg tgctcggaag gccaagccgg
     1981 attattgagt cacagcgcag gtttaatcga aattttcgtg gtgcagggat gatgagggcg
     2041 catcgggccc tcggcaaatg ttgcctgaca cgctgagcag gcgcgactga gttagagtga
     2101 ggattggcgt gagcgatcca aaacaacgag acctcgattc cgttggtcgt gcggcgtcgt
     2161 ggctgcgcga acgtagcaaa gacccggacc cgataccgtt agggcgcgca aaggacaggc
     2221 cggactggcc ggcgcagggc aagcccgatg agaccgtcac gcatttgcgc cccgtcgaac
     2281 cccgggactg gcaaattcat aagcagtcgg atgtctttgc agaggcggtc gcaagggcca
     2341 tgcaagaaca gggtgagcct gatctcgtcg aagcgccatc tgcgctccag aaccgccacg
     2401 cgttcggcag cattgccgcc agatttgcgg ttgccatggg cgtgtccgct ctcgtcgcgc
     2461 tggttttcgt tgctgccttt ccggcctcgc aaggtccggc cgatgaggat ggattagctt
     2521 cgctgccggc tttgaaatcg ttgaggtcat ccctgtttcc ggcgccgcag cgcaagcccg
     2581 cccctagtct ggtcatccgc gacggcagtg gacccgtcaa tgagcggctg tcgcttggcg
     2641 tcaacgtcaa atctcccgat cccggcgcga cggtcacaat cgacagaatg ccggccggtg
     2701 ccaggctgac ggtcggcagg cgcatgagtg ccggcgaatg gcgcgtgcca gcgcaggaga
     2761 tttcagacgc atcggtcatt ccaccggcgg actttgtcgg cgtgatgaat ttgaccgccg
     2821 agcttggtgg ctccgacggg gctgcgctgg tcagcagcgt cgtccggctg acctggacgt
     2881 cagcgcggcc cggcggcccc gttggggggg cgtccgccag cgccgcaact ttgccctcca
     2941 gccccgccgc gccaacgctg caacagcagc cacaacagca acagcccgtg gcgtccccag
     3001 gaccacccgt tgttgtcgcg cgcgcggagc cgccagtgcg ggagatcaat cccaacgaag
     3061 tcgcagattt cgtcaggcgg gcgcaggagc tgctggcgac cggcgatctg caggcggccc
     3121 gcttgttgct gcttcgcgca gcccaagcgc acgacggccg cgccgccctg ttactggcga
     3181 agagtttcga tccgattgtg tcgaagcgaa ttggcatggc cgacgcggag cctgatctgg
     3241 cacaagcccg gaactggtat cagaaagccg aggaatgggg tgcgtccgag gcgcgacgcg
     3301 aactcgatgc gcttgccagc tatccgcgct gacacagaac gcgacccgcg gcgaacggta
     3361 ccgccaaaaa cccacgtctt gagcgatcgt cagcgacgta cgtttaagcg gttcgcgcgc
     3421 cactcgagga attcgcgata cagcggatcg tcgcgatccg gcggcttgtc tgcgcgtggc
     3481 acctcaactg cggcggacgc cgcagttgtc ccgaccgagg gagagttgcg gtcgatccac
     3541 tcctgcgccg cctgaaaccg ggtccagccg gctaccggag ccttcagggc cacctctttc
     3601 cattttggat ggaacggcgg ccgctgcaat tgcgcaactc gcgtaaagag cgaatcgacc
     3661 agcaaggaga gacggcgata gcggtcgctg gtcggttgcc agttgtagga cgccagaaca
     3721 gcctcggccg caatcgtatc gacgggagcg gcgtctgaaa ccaggttggg atagtccgcc
     3781 gatgaaagct gcgacggcaa atactcctcc tggagcgttt tctcatattc gaccggcacc
     3841 aaatgcaggt tgggatccct cacctggctg agccattgca ccggtttgcc ttcgaccgcc
     3901 acgatggcat cgatctcgcc tctactcagc tgttccagtg cgagccgcgg ttcgatatag
     3961 agcagatgcg gcttgatccc gagacgctcg aatacattga tcgaggtcac gaaggtgctg
     4021 gcatccggca aatcgacgac caccgtcttg ccatcgagat ccttcaggct gcggatcgac
     4081 cgcggcgcga gcacgtgcat ttcctcgttg aacatcttgg tcacgtagac aaactgattg
     4141 cggatattgt tggcgaagtc cttgcgctca aggtacgcga gcgtgtcctt gcgcacgatt
     4201 ccggcgtcca cccccttcaa taacaggata tccgccacgg cctgaaccga tccgcggccg
     4261 agaataggca acacgcgcag atttgcgcca ttgtccagca ctgaagagag atcggcgccg
     4321 atctgggcat aagtactgcc gaacgagccg gtcatcaggg ttacggtgtt ggcgttcagc
     4381 tgagccgcca tgtcgtgctt ggctgtaccg taacggaaga tcgtcttgaa ggagtcgctg
     4441 acgctgcccg catcaatccc gctgcgaacg gatccgaagc gttcccatcg tccggctgag
     4501 aaccgcatca tctgcatctg ctcaagcggc gcgtgatcgc tcgcgcttgt attgaccacg
     4561 atcccgggga gcagcattgg aagctgcaca cccttgagcg aggccgcctg ccgcatcaca
     4621 ttctctcggg acagatcatc gccgcatctc accagcacct cgaccatcgc cttggcgatc
     4681 acgtaaccga agacactcag gctgttggtc ttgcttgcgc tcggaacata tcggtccatg
     4741 aaggcggccc agtcgcggta aaccggatcg ctggcggccg ccggatcatc gccctcgaaa
     4801 ctgtatccag ccgacagcag tccctccgca ttctgcgggc ccgccggctg catcaccgcc
     4861 gagaccgatt cagacaccgt cgggatgata tgtaccggtt tccaccccag ttcggcggtg
     4921 cgccggatca ccatgacagc gaatttcggg gtcgtcacat cgaagaaaat gtcggcgccc
     4981 gaggccttga gtttggtaat ctgcgcatcg acggtcgggt ccgtgacctt gtacggagcc
     5041 tcagccacga tcgggatctt gccgcctagt ccgtccttca ggcccttgag gtagtccttg
     5101 ccgaacccat cgtcttgata aagcacggcg atcttgccgt gcggatggtt ctccagcaaa
     5161 tactgcgcat agatgtgagc ttcggtctgg tagctgggct ggaagcccat ggtccagggg
     5221 aactcgtgcg gctggtccca catcgacgcg cccgaagcga taaacagctg tgggaccttt
     5281 tttgagttca aatagggttg aaccgcggca cttggcgccg tgccgaggct cgaaaagacc
     5341 aggaggaccc ggtcctcctc gacaagcttg cgggtcagct ccacggtctt ggcggggttg
     5401 tacgcatcgt catacgatat gaagttgatc ttgcggccat tgatgccgcc ttcggcgttg
     5461 atcttgtcga agtaggccga gattaccttg ccaatggtgc cgtaggcgga agccggaccg
     5521 ctgtacggca tagtgttgcc gatccggatc tcgttgtcgc ccgcgctcgc agcggaaaca
     5581 cgctccgccg ccagcggccc cgccatgaag gcgacgatca gcgctgtcgc aacgttaggc
     5641 cacctcacgg cgcgccgcca acgaaccggt gcactggcca gcggccatcg cacttctaat
     5701 gcacggcaca caacgtcgat catggtccgc atcgtcgcac ctcaattgtg ctgttactgg
     5761 tgcatttttg gtgagcgctg cttcaaacaa cgtggactag ggtcacggca ctacccgcca
     5821 acattgtgtc tcgattagat cgattatgtg tcgaggtcag gccatgaagc ccggcatcac
     5881 cggcaagtcg gacgtattca ggagagcata ctggttcaac cgcgccaccc agtcatcgga
     5941 tcaagtacct caaggtcgat gccgacaccg gcgaggacgt cgcgtccaac gagaccatga
     6001 agtgcaaaaa ggttgatacc gacacctaca cgaaggacga gccggaaaac atcgcgctct
     6061 ctccatcttt aggctccgga tgacccgcca gattcagcaa agacctcgga ggcaatgcgg
     6121 ttcgtttcga ttgcggcggg tacgccgcaa tacatcgcga ccagcaacag gacgtcctgc
     6181 acctgctgct tcgtgcatcc gtttgccagc gcgccctttg catgaacgcg gaattccgcc
     6241 ggccgattgc tcgcggcggt tattccgagc atcacgagac tgcgaatatc gtcggcaagg
     6301 ccatctcttt cccagatgtc gccgtaaaca taggcattga tgatgttttg cagaggcgcg
     6361 ccgaactcgc cggaagcagt catccgcttt tcgacgtcgg cctcaccaaa cattcgcttt
     6421 cgccgtcgca atccgcgtgc gtacatttcg ccagtatcca cgttgtcttc cccctgtcgt
     6481 aggtgattaa gctcatgtac acatgagccc ttcctgtatt tgtacccata aaacccttgg
     6541 cgtgaaatcc ataaagcaag tggtggcgac aatgcgcagg ctaatgatgg tgcgattggg
     6601 ccttgttgcc tgccttgtcc tgccgggagt gttatcgccg caactcgcgg tcgcggtcga
     6661 cgattatccg aaccgacaga tcaagatcat cgtgccattt cctgccggtg cgggtccgga
     6721 tcagatcgca cgcatgctcg gccagcacct gcaggacgcg tttggacaga cggttgtgat
     6781 cgagaaccgg accggtgcgc ttggcagcat cggcgcgcag gaggtcgccc gcagcgcgcc
     6841 tgacggctac acgctcctga tgggaaccaa cacgacgcag gcagcgaacg tcgcgatgct
     6901 gaaaaatctg tcctacgatc cggccaagga ctttgcgccg atcattcgga cgataacaac
     6961 cgcgatggtg ctggtcgtga agccggactt tccggccaaa aacctggctc aattcatcga
     7021 gtatgcacgc gctcacccga acgtgaccgc cggctacggc tcgggggcat cgcagatatc
     7081 gatcggacaa ctgcaaacgc gcggcggcat atcggtgatc gcggcttcct atcggggtgt
     7141 accgcaggcg gtcaccgacg tgatcgccgg cgtgatcgac cttgcctttg ccgatttttc
     7201 gatcgcaatc ccgcaattac agggtggtac gctgcgcggc attggcgtga catcgccgaa
     7261 gcgaaacgac ttgacaccgg atcttccctc gcttgccgag gccatgcccg gtttcgaggc
     7321 aacgatctgg tacgggttac tggcgccggc tggtacgccc gaaccgatcg tgaacaagct
     7381 ctatgacgag agtgaaaagt tcctgacgat gcccggaacg cgggagaagc ttgcaggtgt
     7441 cggcgtgatt gtctcgccga tgcgccctga cgaattcggc gcctttatcc gcagcgagat
     7501 caagcgatgg acggcggatg ccaaggctgc cggcatcgag gcgaaatagt cacgttatcg
     7561 gaaatggcga gctgatcatg caagtgaatg ggccgatcgg aattatcgga ctaggagccc
     7621 gtccagatag gttttgtgac ggacatttgt tgcagggtag ccggctcagg ctgcgtttgc
     7681 gagggtgaac agcttcagga ggttgtgggc ggtgcagatc atcgcccact cggcgcgcac
     7741 tttctcgacg c
//
LOCUS       DASWGM010001015         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_410658,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001015 DASWGM010000000
VERSION     DASWGM010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410658"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1522
                     /locus_tag="VGA15_27370"
     CDS             77..1522
                     /locus_tag="VGA15_27370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX9591449.1"
                     /translation="MERTHLLTSFQDFGLADPISRALKEENYLTPTPIQAQTIPIALT
                     GRDVVGIAQTGTGKTAAFALPILHRILENRVKPQPKACRVLVLSPTRELSGQILESFN
                     AYGRHMRLTSALAIGGVPMGRQVRSIMQGVEVMVATPGRLLDLVQSNGLKLGQVEFLV
                     LDEADRMLDMGFINDIRKIVAKLPVKRQTLFFSATMPKDIAELAEQMLRDPARVAVTP
                     VASTVERITQRIIQVDHSAKPALLAQLLKQEPVDRALIFTRTKHGADKVVKGLARAGI
                     TADAIHGNKSQNHRERVLAAFRSGEIRTLVATDIAARGIDVDGISHVVNFDLPNVPET
                     YVHRIGRTARAGADGVAISLCAGEEMAFLRDIEKLIRITLPREDRRTPGHRDTAPAPA
                     QHRGARSGPRVHAARGNDAAPGSKGPRRHRRPGGNNNAPPTNRHEANRQEPSRTSSQA
                     GGKAEGMQGVAFLHRESRPNTRPNRTQRPQR"
     gene            1544..1825
                     /gene="infA"
                     /locus_tag="VGA15_27375"
     CDS             1544..1825
                     /gene="infA"
                     /locus_tag="VGA15_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX9591450.1"
                     /translation="MAKEELIQFEGLVTEILPDARYRVQLDAGHEIVAYTAGKMKKNR
                     IKTLAGDRVTIEMSPYDLEKGRLIFRHKDERPGGGGPRGAPPRGQFRRR"
BASE COUNT          396 a          694 c          604 g          321 t
ORIGIN      
        1 cgccccaaac acgcgggtgt ctctgacacc ccctcgcgcg ctcctggcga ttaccgtcgg
       61 gcgcctgcca ttcataatgg aaagaaccca ccttttgacc tcctttcagg atttcggtct
      121 cgccgatccc atctcgcgtg cgctcaaaga agagaattac ctcacgccta cgcccatcca
      181 ggctcaaacc attcccatcg cactgactgg ccgtgacgtc gtcggcattg cccagaccgg
      241 caccggcaag accgccgcct tcgcgctgcc gatcctgcat cgcatcctcg agaaccgcgt
      301 caagccgcag cccaaggcct gccgggtgct cgtgctcagc ccgacccgcg aattgtcggg
      361 acagattctc gagagcttca atgcctatgg ccgtcacatg cgcctcactt cggcgctggc
      421 catcggcggc gtcccgatgg gacgtcaggt ccgctcgatc atgcagggcg tcgaagtcat
      481 ggtggccaca ccgggccgcc tgctcgacct cgtgcaaagc aacgggctga agctcggaca
      541 ggtcgagttt ctcgtgctcg acgaggccga ccgcatgctg gacatgggct tcatcaacga
      601 catccggaaa atcgtcgcca aattgccggt caagcggcag acgctgttct tctccgcgac
      661 gatgcccaag gatatcgccg agctggccga acagatgctg cgcgatccgg cgcgggtggc
      721 cgtgacaccg gtcgcctcga cggtcgagcg cattacccag cgcatcatcc aggtcgacca
      781 ctcggccaag cccgcattgc tggcgcagct cttgaagcag gagccggtcg atcgcgcgct
      841 gatctttacc cggaccaagc atggcgccga caaggtggtg aagggcctcg ccagggccgg
      901 catcacggcc gacgccattc acggcaacaa gtcgcagaac caccgcgagc gggtgctggc
      961 ggcattccgc tccggcgaga tccgcacgct ggtcgccacc gacatcgccg cccgcggcat
     1021 cgacgtcgac ggcatcagcc atgtggtgaa cttcgatctt cccaacgtgc ccgaaaccta
     1081 tgtccatcgc atcggccgca ccgcgcgcgc cggcgccgac ggcgtcgcca tctcgctgtg
     1141 cgcgggagag gaaatggcct tcctgcgcga tatcgaaaag ctgatccgca tcaccttgcc
     1201 cagggaagac cgccgcacgc cgggtcatcg cgacaccgcg ccggcgccgg cgcagcatcg
     1261 aggtgcacgg tccggaccgc gcgtgcacgc ggcccgaggc aatgacgcag cccccggttc
     1321 gaaagggcct cgccgtcacc gccgtcccgg tggtaataac aatgctccgc caaccaaccg
     1381 gcacgaggcc aaccggcagg agccgtcgcg cacctcgtcg caggctggcg gcaaggctga
     1441 agggatgcag ggggttgcct ttttgcatcg cgaaagccgt ccgaataccc gacccaaccg
     1501 cacccaacga cctcagcgct agccgatcga tctggagaca accatggcta aagaagagct
     1561 gatccagttc gaaggactgg tgaccgaaat cctccccgat gcccgctacc gcgtgcagct
     1621 cgatgccgga cacgagatcg tcgcctacac cgccggcaag atgaagaaga accggatcaa
     1681 gacgctggcg ggcgatcggg tgacgatcga gatgtcgccg tatgatctgg agaagggccg
     1741 gcttatcttc cgtcacaagg acgagcggcc gggcggcgga ggcccgcgtg gtgcaccgcc
     1801 gcgcggccag ttccggcgca ggtaaacgcc ccccgcagct acagcgaaat cgcgcatgac
     1861 gagatcacct cggtctcggt gcgcggtgac ggcgcgccgc cacgaaaccg ctgcaattaa
     1921 aaaaagcgcg cacgtcagga ttgttttggg ggctgccttc gcataatatg tattaatcga
     1981 ttttcggccg gacgacatta gctatccacc ggtac
//
LOCUS       DASWGM010001016         2888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_410725,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001016 DASWGM010000000
VERSION     DASWGM010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410725"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /gene="oxlT"
                     /locus_tag="VGA15_27380"
     CDS             <1..1105
                     /gene="oxlT"
                     /locus_tag="VGA15_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEX9591451.1"
                     /translation="FTLFVLFETWLVPIEGWFVDKYGPRLVVLIAGILCAIGWAVNAE
                     ATSLNGYYLGMIIAGIGAGGVYGTCVGNALKWFPDKRGLAAGITAAGFGAGSALTVAP
                     IQAMIKDSGFQSTFLYFGLGQGIIIVILAFFLFAPKAGQVPAVVQNANIIQTRRNYQP
                     TEVIRHPIFWLMYFMFVIVGAGGLMVTANLKPIAVDWKIDNTPVTLMAMTMTAVTFAA
                     TLDRILNGLTRPFFGWVSDQIGRENTMFIAFGVEGIGIWTLYMLGHDPVWFVILSGLV
                     FFAWGEIYSLFPSTCTDTFGSKFATTNAGLLYTAKGTAALLVPVANYMQQSSGSWDLV
                     FLIAAGANILASLLALVVLKPWRRIVVAKSQAS"
     gene            1392..1826
                     /locus_tag="VGA15_27385"
     CDS             1392..1826
                     /locus_tag="VGA15_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX9591452.1"
                     /translation="MQVGDILRKKTARVATVRMNETVAIAAQLMRASNISALVVKDVV
                     RTEGNTAVGMFTERDVVRAIAEHGAAGVNLKVSQFVSVQQLVSCSSTDTLEHIRHLMN
                     KNHIRHLPVIDDYSLIGVVSIRDISTAFDDQATGAARSASAA"
     gene            1941..>2888
                     /locus_tag="VGA15_27390"
     CDS             1941..>2888
                     /locus_tag="VGA15_27390"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX9591453.1"
                     /translation="MKICIYGAGAIGGYLGVQLARAATDVSLVARGAHLAAMRENGLK
                     LLIGDQQHVVRPRCTDNPAELGPQDFVIVCLKAHSITGVIEAMQPLLGPRTRIVTAVN
                     GIPYWYFYKHGGNFEGSTLESIDPGGRQWRELTPQRAIGCIVYPATEIEAPGVIRHVY
                     GDRFPLGEPSGAISPDVERLSAVFVEAGMQAPVLERIRDEIWLKLWGNVCLNPISALT
                     GATLDVICSDPATRALSKAIMLETQAIAETYGVKFRVDVERRIDGARKVGAHKTSMLQ
                     DLERGRPMEIDPLVSVVQEMGRLTQIPTPALDAVLALVAQ"
BASE COUNT          534 a          896 c          846 g          612 t
ORIGIN      
        1 attcacgctg ttcgtgctgt tcgaaacctg gctggtcccg atcgaaggct ggtttgtcga
       61 caaatacggc ccgcgcctgg tggttctgat cgccggcatc ctttgcgcca tcggatgggc
      121 tgttaacgcc gaggcaacct cactcaacgg ctattatctc gggatgatca tcgccgggat
      181 cggtgccggc ggcgtctacg gcacctgcgt cggcaacgcg ctgaaatggt ttccggacaa
      241 gcgcggcctt gccgcgggca ttaccgcggc gggatttggc gcgggttcgg cactgacagt
      301 cgcgccgatc caggcgatga tcaaggattc cggtttccag tccacattcc tttatttcgg
      361 tcttggacaa ggcatcatta tcgtgatcct cgcgttcttc ctgttcgcac cgaaggcggg
      421 acaggtcccc gccgttgttc agaatgccaa cataatccag acccgtcgca actatcagcc
      481 gacggaagtg atccgtcatc cgatcttctg gctgatgtat ttcatgttcg tgatcgtcgg
      541 cgccggcgga ttgatggtca ctgccaatct caaaccgatc gccgtcgatt ggaagatcga
      601 caacactccg gtgacattga tggcgatgac aatgacggcg gtgacgttcg cggcaaccct
      661 cgatcgcatt ctcaacggtc tgacccgccc gttcttcggc tgggtctccg atcagatcgg
      721 ccgcgagaac acgatgttca tcgctttcgg cgtcgagggt atcggcatct ggacgctcta
      781 tatgctcggc cacgatcccg tctggttcgt gatcctatcc gggctggtat tcttcgcctg
      841 gggtgagatc tactcgctct tcccatcgac ctgcaccgac accttcggct cgaaattcgc
      901 gaccacgaac gccgggctgc tctacaccgc caaaggcacc gcggccctgc tggtgccggt
      961 cgcgaattac atgcagcaat cgtccggctc atgggatctc gtgttcctta tcgcagcagg
     1021 ggccaacatt ctggcttcgc tgctggcgct cgttgtgctc aagccgtggc gcaggattgt
     1081 cgtcgccaaa tcccaggcgt cttaacccgt cctgcaccgc ctgctgaaaa agcggcgctc
     1141 atccaagggg tgggcgcttt tttttgcgcg ggtcgcggtc gctggcatac ctggggcctt
     1201 gcggcgcctc ctggcagatc gcatcttccc gatggaagca acacagaatg ctttggaacg
     1261 tcagtcttac tgccactttc tttgctttca accgtggcat gccaaccgcg attccaattt
     1321 gacaattggc ataatgtata ccaatctagt cacgtgagag gcgccctgcg gctcaattga
     1381 aggaggttgc gatgcaagtc ggagacatcc tgcgcaagaa aactgcccgc gttgcgacgg
     1441 ttcgaatgaa cgaaaccgtc gccattgccg cccagctgat gcgcgccagc aatatcagcg
     1501 cgcttgtggt caaggacgtt gtccggaccg aaggcaatac cgcggttggc atgtttaccg
     1561 aacgcgacgt cgttcgcgcc attgccgaac acggcgctgc cggcgtcaac ctgaaagtct
     1621 cgcaatttgt ttcagtgcag cagttggtgt cctgcagttc gaccgatacg ctcgaacata
     1681 ttcgtcatct catgaacaag aaccacatcc gccatctgcc tgtcatcgac gattacagcc
     1741 tgattggcgt cgtcagcatc cgcgacatct caaccgcgtt cgacgaccaa gcaacgggcg
     1801 cggcacgatc ggcctccgct gcctgaggaa accggctccc cgccccgccg gccgcgattt
     1861 gaccctggct ttgagatttc gctcacagcc agcgcgtgtc ctgtgcggaa attttccgcc
     1921 acccgcattc gaaggaattc atgaaaatct gtatctacgg cgccggcgcg atcggcggat
     1981 atctcggcgt gcagctcgcg cgcgcagcca ccgacgtcag ccttgttgcg cgcggcgcgc
     2041 atcttgccgc gatgcgcgag aacggcctca aattgctgat cggagaccaa cagcacgtgg
     2101 tgcggccgcg ctgcaccgac aatcccgccg aactcgggcc gcaggatttc gtgatcgttt
     2161 gcctgaaggc gcattccatc accggcgtca tcgaggcgat gcagccattg ctcggaccgc
     2221 gaacgcgcat cgtcaccgcg gtcaacggaa ttccctattg gtatttctac aagcacggcg
     2281 gcaattttga gggttcgacg cttgagagca tcgatcccgg cggccggcag tggcgtgagc
     2341 ttaccccgca gcgcgccatc ggatgcatcg tctatcccgc gaccgagatc gaggcgcccg
     2401 gcgtgatccg ccatgtctac ggcgatcgct ttccgctcgg cgagccgtcg ggagcgatca
     2461 gtccggacgt cgaacggctt tccgctgtct tcgtcgaagc cggcatgcag gcgccggtgc
     2521 tcgagcgcat ccgcgacgag atctggctca aactgtgggg caatgtgtgc ctcaatccga
     2581 tcagcgcgct gaccggtgcg acgctcgacg tgatctgctc cgatccggcc acgcgcgcgc
     2641 tgtcgaaggc gatcatgctg gaaacccagg cgatcgccga aacgtatggc gtcaaattcc
     2701 gtgtcgatgt cgaacggcgg atcgacggcg cgcgcaaggt cggtgcacac aagacctcga
     2761 tgctgcagga tctcgaacgc ggacgcccga tggagatcga cccgctggtg tctgtggttc
     2821 aggaaatggg gcgattgaca cagattccga caccggctct ggatgcggtg ctggcgctgg
     2881 ttgcacag
//
LOCUS       DASWGM010001017         9362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_535854,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001017 DASWGM010000000
VERSION     DASWGM010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535854"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VGA15_27395"
     CDS             <1..1165
                     /locus_tag="VGA15_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591454.1"
                     /translation="GTAANAQISDDVVKIGVLTDMSSLYADATGKGSLAAVQMAVADY
                     GAKVKGKPVEVISADHQNKPDIGVGIARNWYDNEKVDAIFDVPTSSVALPVSALTREK
                     NKININSGGGSSDITGAACSPNTVHWTYDTYALSNVAGRAMVKRGEDSWFFVTADYAF
                     GMALERDAANVVKESGGKVVGNVRHPLNSSDFSSFLLQAQASKAKVVALANAGGDTTN
                     ALKQASEFGITKGGQKMIALLQEITDTHALGAKETQGLIVTDAFYWDMNDETRAFSRR
                     FNEKVGHMPTMIQAGLYSATMHYLKAIEAIGTDEAPKVMEQMRATPINDFFAHGGKIR
                     IDGRMVHDMYLFEVKKPEESKGEWDLYKLLATVPGDEAFRPLDKGGCPLVKMN"
     gene            complement(1387..1923)
                     /locus_tag="VGA15_27400"
     CDS             complement(1387..1923)
                     /locus_tag="VGA15_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX9591455.1"
                     /translation="MLAKYMTAGLVGSALLATVAFAQNPTATTDRANMAPAAASDSSF
                     QGDWRASKVVGVSVYNENNESLGSINDLLMDKSGNIKAVVIGVGGFLGVGEHLVAIPF
                     DKIKFVNEPVAYTGVAGAPNAPGSRPASSTTTGAATTNTPPAAAAMPKSNPWYPDHAV
                     FNATKDELKAMPEFKYST"
     gene            complement(2111..2485)
                     /locus_tag="VGA15_27405"
     CDS             complement(2111..2485)
                     /locus_tag="VGA15_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX9591456.1"
                     /translation="MTDVMTKPHQLIASDRVEGTAVRRPNGDKLGHIERLMIDKISGK
                     VSYAVLSFGGFLGMGATLLPLPWARLTYNTRFEAYQLDIDDEELKRAPSFRSDMDFDW
                     GDRSQEAELHRYYGTPPYWGGF"
     gene            complement(2724..3320)
                     /locus_tag="VGA15_27410"
     CDS             complement(2724..3320)
                     /locus_tag="VGA15_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591457.1"
                     /translation="MTSISATSSTTYQSPLQRLQDELQTEVSSGAISSSDQSALSSAL
                     NDIDSSLQSGQSSGQSCGTQSSPGDLKSKIDDLIAGEVSSGKLTSDQATELQGVFKAA
                     FANGPDGAGGPGGAGGAHGHHGGQGGPPPADSASSSDSTSSSSGTSSASDILQQFLQS
                     LQDSLSASSSTPYSATGTSGTGSNSSTSFSALLIDYQT"
     gene            3491..4219
                     /locus_tag="VGA15_27415"
     CDS             3491..4219
                     /locus_tag="VGA15_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9591458.1"
                     /translation="MATASPNILVVEDDRETRTLIAKYLRTNACNVTTANDGREMVRA
                     MTDHRVDLLILDVMLPGEDGLSLCRKVRAESQMPIIMLTARGEDVDRIVGLEMGADDY
                     LAKPFNPRELLARINAVLRRQAAAQTLSAANGATALTFLGWQIDFRLRELRNPAGARV
                     AMTSAEFDLLRTFCERPGRVLSRDSLLDLTQGRNAGSFERSIDVLVSRIRRKIETDPL
                     EPTMIKTVRSGGYMFTPRVEAVTS"
     gene            4222..5550
                     /locus_tag="VGA15_27420"
     CDS             4222..5550
                     /locus_tag="VGA15_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9591459.1"
                     /translation="MKVGFFNLKGISGQIAALVVASIIAIHLILTATFLIHRPDQPDP
                     SIDRGHAQLVASVQLLGAAPAAERPRLFADIARAFPQLDIASLPPGAAAAANEPDGFN
                     LHGLRHRLGGSYRIFSLAHEENSQKVGITLPDGAMISAKILPDQRQPPFWGGPWMMTL
                     LFAVISVTLLGVWAARALTAPLSSFAKAAESFSLNGATAPLPERGPEEIRSVAKAFNR
                     MRERITALIDDRTKMLAAISHDLRTPITRMRLRSEFIEDDGHRSRMLRDLDQMRSMLE
                     SVLSFLRNDRKLESMTLVDIATTLQLVTDQFADMGHKVGYDGPAHAMATVRPDDLHRS
                     VTNLVENAVRFGAETTIRLRVSSDTATIDIEDDGPGISDARKGVMLEPFVRGDDARNM
                     DEAAGFGLGLSIAHAIVLAHGGELSLNDRQPHGLMVRIQLPVRQQSRQAA"
     gene            complement(5577..5777)
                     /locus_tag="VGA15_27425"
     CDS             complement(5577..5777)
                     /locus_tag="VGA15_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591460.1"
                     /translation="MAKMDFDDLPHGNPCAQCGKPIAAPEWIETGPRRTSYLWHCRAC
                     DYRFEAVAFFSDTQPDHKALAA"
     gene            complement(6077..7570)
                     /locus_tag="VGA15_27430"
     CDS             complement(6077..7570)
                     /locus_tag="VGA15_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591461.1"
                     /translation="MTLSVANIAEDASPMRGLLPLWVGAFIYALFLLAGNRLLIDPDT
                     MWQITVGQWILDHRAVPETDIYSFTMRGQPWISTQWLAQVLYAKAYAAAGWSGPVVLA
                     ASAIAATFALLTKFLCKRLSESTTLVFVAAALALTVPHLLARPHVLALPIMVAWVGGL
                     IAAADRRGAPSFGLLPLMALWANLHGGFVFGLVLIAPIALDAVLSADLKLRKSLALRW
                     AAFGVAALGLSCATPYGWNSLLASQKILGLGGALPLIMEWKPADFGSVGAFEVCLLLG
                     IGLALFRGVKLPPLRIVLLLGLLHMALAQGRAAEILALLAPLVLAAPLAGPIGGTELS
                     NPAAAPPMRGVLFISVAIALLAGTFAYASVHRFEPHTRGSPVAAVTALKQLNLTRVFN
                     DYDFGGYLIANGVAPFIDGRTELYGEKFFLDHNAASGLMEPENLFRLLDEYKIEATLM
                     RTQSAATKLLDHIDGWQKVFADDIATIQLRMPGAVHTIEPAVDTKPK"
     gene            7768..8223
                     /locus_tag="VGA15_27435"
     CDS             7768..8223
                     /locus_tag="VGA15_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX9591462.1"
                     /translation="MKRHFFLAGLAAIVALPFPQAALAQRAQYEALIASHAQANGVPE
                     ALVHRVIVRESRYQPHLIGRGGTIGLMQIKLATARGLGYTGTADGLRDPNTNLTYAVK
                     YLAGAYRAANGDHNRAVAYYASGYYYAAKRQRHEHIRHSEPMLASAPAK"
     regulatory      8389..8524
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8512..>9362
                     /locus_tag="VGA15_27440"
     CDS             8512..>9362
                     /locus_tag="VGA15_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9591463.1"
                     /translation="MYQRPESSRGESPVSVIGAGIAGAWQALLFARAGHRVTLHERSD
                     AAMTQSTSTWAGGMLAPWCEAETSEPVISRLGIRSLDLWREHFPKTPFNGSLVVAHPR
                     DRGDFERFARLTSGHRRLDAQAVTELEPSLEDRFREGLFYAGEGHVEPRRVLPELHAR
                     FTAAGGTIQFNSEADASELDGIVIDCRGLAGRDTQPELRGVKGEMIVVETDEVELSRP
                     VRLIHPRWPLYVIPRGGNRFMLGATSIEAEDNGVSVRSALELLGAAYAVHPAFAEARI
                     VEFGSGLR"
BASE COUNT         1784 a         2989 c         2923 g         1666 t
ORIGIN      
        1 cggcaccgcg gcgaatgcgc agatctcgga cgacgtcgtc aagatcggcg tcctcaccga
       61 tatgtccagc ctctatgccg acgccaccgg caagggctcg ctggccgccg tccagatggc
      121 ggttgccgac tacggcgcca aagtgaaggg caaacccgtc gaggtgattt ccgccgacca
      181 tcagaacaag cccgacatcg gcgttggcat cgcgcgcaac tggtatgaca acgagaaggt
      241 cgacgcgatc ttcgacgtgc cgacctcctc ggtcgctttg ccggtttccg cgctgacccg
      301 cgagaagaac aagatcaaca tcaattctgg cggcggcagt tccgacatca ccggtgcggc
      361 ctgttcgccg aacaccgtgc actggaccta cgatacctac gcgctctcca acgtcgccgg
      421 ccgggcgatg gtcaagcgcg gcgaggacag ctggttcttc gtcaccgccg actatgcctt
      481 cgggatggcg ctggagcgcg atgcggcgaa tgtcgtcaag gagagcggcg gcaaggtggt
      541 cggaaacgtc cgccatccgc tcaattcgtc ggacttctcg tccttcctgc tgcaggcgca
      601 ggcctccaag gccaaggtcg tggcgctcgc caacgccggc ggcgacacca ccaatgcgct
      661 caagcaggct tccgaattcg gcatcaccaa gggcggccag aagatgatcg cgctgctgca
      721 ggaaatcacc gacacccacg cgctcggcgc caaggaaacc cagggcctga tcgtcaccga
      781 cgccttctac tgggacatga acgacgagac ccgcgccttc tcgaggcgct tcaatgagaa
      841 ggtcggccac atgcccacca tgattcaggc cgggctgtat tcggcaacca tgcactatct
      901 gaaggcgatc gaggcgatcg gtaccgacga agcgccgaag gtgatggagc agatgcgggc
      961 gacgccgatc aacgactttt tcgcacacgg cggcaagatc cgcatcgacg gccgcatggt
     1021 ccacgacatg tatctgttcg aggtgaagaa gccggaagag tcgaagggtg aatgggatct
     1081 ctacaagctg ctcgccaccg tacccggcga cgaagccttc cgtccgctcg acaagggcgg
     1141 ctgcccgctg gtgaagatga attgaggcgg ctcctcttct ccctcgcccc gctcttgcgg
     1201 ggagagggtc ggggtgaggg gctcttgccg cgaattgtga agatgtggtg aggccggtac
     1261 cccctcaccc gccgcgcaag agcgcggcga cctctccccg caagcggggc gaggtaagca
     1321 aactgaaaaa acgcgcccgg caaaaccggg cgcgcttcta tttgacaggt ctattcaggc
     1381 attgcctcaa gtcgaatact tgaattccgg catcgccttc agctcatcct tggtcgcgtt
     1441 gaacacggcg tggtccggat accacggatt ggacttcggc attgccgccg cggccggcgg
     1501 agtgttcgtg gttgcagcac ccgtggtcgt cgatgacgcc ggccggctgc cgggcgcatt
     1561 cggcgcgccc gcaacaccgg tgtaagcgac cggctcgttg acgaatttga tcttgtcgaa
     1621 gggaatggcg accagatgtt cgccgacccc gaggaagccg ccgacgccaa tcaccaccgc
     1681 cttgatgttt ccgctcttgt ccatcaacag atcgttgatc gaaccgaggc tttcgttgtt
     1741 ttcgttgtag acgctgacgc cgacgacctt ggacgcacgc cagtcaccct ggaacgacga
     1801 gtccgaagcg gcagccggcg ccatgttggc gcggtcggtg gttgcggtcg ggttttgcgc
     1861 gaacgcgacg gtcgcaagca atgcggaccc gaccagaccg gccgtcatat attttgctag
     1921 catgggctgt ctccctgata tgaaattggt ggggagaaaa cacgccgatc cgtcggtcgt
     1981 tcccaatcgc cgaatacaat ttaaccgtta gccgtgccga cgatgggaga gttccataca
     2041 acatcgctgc gcacacgcgc gcacggcgac cgcgtgccac attgctgcta tgcccacgtt
     2101 tcaatcgcga tcagaaaccg ccccaatatg gcggtgttcc gtaatagcgg tgcaactccg
     2161 cttcttgcga acgatcgccc cagtcgaaat ccatgtcgga gcggaatgac ggcgcgcgct
     2221 taagttcctc atcgtcgatg tcgagctggt aggcctcgaa cctggtgttg taggtgagac
     2281 gagcccatgg cagcggaagc agggtggctc ccatgccgag gaacccgccg aaactgagca
     2341 ccgcgtagga caccttgccg ctgatcttgt cgatcatcag ccgttcgatg tggccgagct
     2401 tgtcgccgtt cggccgacgc accgcggtac cctcgacgcg gtcgctcgcg atcagttggt
     2461 gtggtttggt cataacgtcg gtcatcgcac cctccttttg ggttgatgaa ttaaatcaac
     2521 gccataacag cggatcggtt ccatcagcac cgggccaaat tgcttgaggg agggcgaagg
     2581 aggaattacg taccgcctcc gggctctcac cggtccggtc tggcgggccg gggactgcgc
     2641 aggccgagaa ccgggcgatc atcctttccc gttctgggaa ggatgatgac gaggtgcgtt
     2701 ggggtgtgcg tcgcggcttg aggtcaggtt tggtagtcga ttagaagcgc agagaacgac
     2761 gtggagctgt tgctgccggt tccggaggtg ccggtcgcgc tatagggcgt agatgacgag
     2821 gccgacagcg aatcctgcaa cgactgcagg aactgctgca gaatgtcact ggcggagctg
     2881 gtgccggacg agctgctcgt gctgtcggac gaggacgcac tgtcggccgg cggtggaccg
     2941 ccctgtccgc cgtgatggcc gtgcgcgccg ccggcaccac ccggaccgcc tgcgccatcg
     3001 ggaccgttgg caaaggctgc cttgaagacg ccctgcagtt cggtggcctg atcgctggtc
     3061 aatttgccgc tggacacttc gccggcaatc aggtcgtcga tcttcgactt cagatcgccg
     3121 ggagaggatt gtgtgccgca ggactgaccg ctcgactggc cgctttgcag cgaggagtcg
     3181 atgtcgttca gcgccgagga caacgcgctc tggtcggacg aactgatggc gccggaactt
     3241 acttccgtct gcagttcgtc ctgcagcctc tgcagaggcg actggtaggt agtgctggag
     3301 gtcgcagaga ttgaggtcat gatggctccg aaggttctta agaagacgcc tcgaaggtag
     3361 ggccacggaa cttaacgagg cctttttatc gaccttccgt gttgttgccc cgtgttgcga
     3421 aggggacgcc agaaacattt ggaaacaaaa atccttcatc gaaccctgcg aatcaccgac
     3481 aaaggtcgtt atggccacgg catcgccgaa cattcttgtc gttgaggacg accgcgagac
     3541 gcggacgttg atcgcgaaat atctgcgcac caacgcctgc aacgtgacga cggcgaacga
     3601 cgggcgcgaa atggtccggg cgatgaccga ccatcgcgtc gaccttctga tcctcgacgt
     3661 gatgctgccg ggcgaggacg gcctcagcct gtgccgcaag gtccgcgccg aatcgcaaat
     3721 gccgatcatc atgctcaccg cgcgcggcga ggacgtcgac cgcattgtcg ggctcgagat
     3781 gggcgcggac gactacctcg ccaagccgtt caacccgcgc gaattgctgg cgcggatcaa
     3841 cgcggtgttg cgccgccagg ccgccgccca gacgctcagt gctgcgaatg gcgcgaccgc
     3901 cctcaccttt ctcggatggc agatcgattt caggctgcgg gagctgcgta acccggcagg
     3961 cgcccgggtt gcgatgacca gcgccgaatt cgacctgctg cggaccttct gcgagcggcc
     4021 gggccgcgtg ctgtcgcgcg acagcctgct cgatctcacc caagggcgca acgccggctc
     4081 gttcgagcgc agcatcgatg tgctggtcag ccgcatccgg cgcaagatcg aaaccgatcc
     4141 gctcgaaccc accatgatca agacggtgcg ttccggcggc tatatgttca cgccccgggt
     4201 ggaagcggtg acgagctgac gatgaaggtc ggttttttca acctgaaggg gatcagcggg
     4261 cagatcgccg cattggtggt tgcctcgatc atcgccattc acctgatcct caccgcgacc
     4321 tttctgatcc accggccgga ccagcccgat ccatcgatcg accgcggcca cgcccagctc
     4381 gtggcatcgg tccagcttct cggcgcggca ccggcggccg agcggccacg gcttttcgcc
     4441 gacatcgcgc gggcattccc gcaactggac atcgcaagcc ttccgccggg agccgctgct
     4501 gcggcaaacg agccggatgg cttcaacctg cacggcctgc gtcaccgcct cggtggcagc
     4561 taccggatct tttcacttgc gcacgaggag aacagccaaa aggtcggcat cacgttgccg
     4621 gacggcgcca tgatttccgc aaagattctg ccggaccagc ggcaacctcc gttctggggc
     4681 gggccatgga tgatgacgct gctgttcgcc gtcatcagcg tcaccttgct cggcgtgtgg
     4741 gccgcccggg ccttgacggc gccgctgtcg tcattcgcaa aagccgccga gagttttagc
     4801 ctgaacggcg ccaccgcgcc gctgcccgaa cgcggcccgg aagagattcg ttcggtggca
     4861 aaggcgttca accgcatgcg cgagcggatc acagccctga tcgacgaccg caccaaaatg
     4921 ctggccgcca tcagccacga tctgcgcacc ccgatcaccc gaatgcggct gcgctccgaa
     4981 ttcatcgagg acgacggcca tcgcagccgg atgctgcggg atctcgacca gatgcggtcg
     5041 atgctggaga gcgtgctttc gtttctgcgc aacgaccgca agctggaatc catgacgctg
     5101 gtcgacatcg cgaccacgct gcagctcgtc accgatcaat tcgccgacat gggacacaag
     5161 gtgggctatg acggccccgc acatgccatg gccacggtcc ggcccgacga tctgcatcgc
     5221 agcgttacca atctcgtgga gaacgcggtc aggttcggcg cggagaccac gatccgcctt
     5281 cgcgtgtcgt cggataccgc gacgatcgac atcgaggacg atggtcccgg catttccgat
     5341 gcgcgcaagg gggtcatgct ggaaccattc gtgcgcggcg acgacgcccg caacatggat
     5401 gaagccgcgg gctttggtct cgggctttcc atcgcgcacg ccatcgtact ggcgcatggc
     5461 ggcgagctgt ccctcaatga ccggcagcct cacggattga tggttcgcat tcagttgccg
     5521 gttcgccagc agagccggca ggcggcctga gcgcgcaccg ccgcccgcat cgaccgtcag
     5581 gccgcgaggg ctttgtgatc cggctgcgtg tcactgaaga acgccaccgc ttcgaaccgg
     5641 tagtcgcagg cccggcaatg ccagagatag gacgtgcggc gcgggccggt ttcgatccac
     5701 tcaggtgctg cgatcggctt gccgcattgg gcgcagggat tcccgtgggg caaatcgtcg
     5761 aagtccattt ttgccatggc gcatctccct gttgctcgct gatatttttt cttgatatga
     5821 ttttttggac aggatggctg tcggaaatac gacgccatct tactgcttat gagtgttggc
     5881 gagcattgcg tcatttcaac ggcgaggcct cgtgtcccgg acgcgacgca gcgttcttca
     5941 cggtgcgtcg cagagccggg acccaactca cagacccaac tctcatgggc cccggatcag
     6001 cagcgcacca cgccgcaaag aagcggcgcg ctgcgcagca tccggggcac gcaattgcct
     6061 ttcggcttgg aggaactcat ttcggctttg tatcaaccgc cggctcgatc gtatgcactg
     6121 caccgggcat acggagttga atagtggcga tgtcgtcggc aaaaactttc tgccagccgt
     6181 cgatgtgatc gagcagtttg gtggcagcac tctgcgtgcg catcagggtt gcctcgatct
     6241 tgtactcatc cagcagccgg aacagatttt ccggttccat caacccgctg gcggcgttgt
     6301 gatcgagaaa gaatttctcg ccataaagtt cggttctgcc gtcgatgaag ggcgcgacgc
     6361 cattggcgat cagataaccg ccgaaatcgt agtcgttgaa cacccgtgtc agattcaatt
     6421 gcttcagcgc ggtgaccgcg gcgaccggcg aacccctcgt gtgcggttcg aagcgatgca
     6481 ccgacgcgta ggcaaaggtt cccgccagca gcgcgattgc gacgcttatg aacagcacgc
     6541 cgcgcatcgg cggcgcggcg gctggatttg aaagctcggt gccgccgatc ggcccggcga
     6601 gcggggccgc gagaaccaag ggcgccagca gcgccagaat ttcggcagca cgtccctggg
     6661 cgagcgccat gtgcagcaat cccagcagca gcacgatgcg cagcggcggc agtttgacgc
     6721 cgcgaaacag cgccagtccg attcccagca gcaggcaaac ttcaaatgca ccgacgctgc
     6781 cgaaatccgc aggcttccat tccatgatca gcggcagcgc gccgccgagg ccaaggattt
     6841 tctgtgacgc cagcagcgaa ttccagccat agggcgtggc gcaacttagc cccagcgccg
     6901 ccacaccgaa tgccgcccat cgcagcgcca gcgatttccg cagcttcaga tccgcgctca
     6961 agacggcatc gagcgcgatc ggcgcgatca gcaccagacc gaacacaaaa ccaccatgca
     7021 gattggccca cagcgccatc aatggcagca acccgaatga cggcgcgccg cgccggtctg
     7081 ccgccgcgat cagtccgccg acccacgcca ccatgatcgg caacgccagc acatgcggcc
     7141 gcgccaacag atgcggcacg gtcaacgcca gcgccgccgc gacgaatacc agcgtcgtgc
     7201 tctcgctcag ccgcttgcag agaaattttg tcagcaacgc gaacgtcgcg gcgatcgcag
     7261 atgccgcaag caccaccggc ccgctccagc ctgctgccgc ataggccttt gcatacaaca
     7321 cttgcgccag ccactgggtc gagatccagg gctggccgcg catggtgaag gaatagatat
     7381 cggtctcggg caccgcgcga tggtccagga tccactgccc gaccgtgatc tgccacatcg
     7441 tatcgggatc gatcagcagc cggtttccgg cgagcagaaa cagcgcatag ataaaggcgc
     7501 cgacccacag cggcagcagg ccgcgcatcg ggcttgcatc ctcggcaata ttggcaacgg
     7561 acagggtcat cggcgcgacc ggttctctag gggttttccg cccgatcgaa gcacgccagg
     7621 gcataattcc tggtaaaccg gccgcgatga atgtggctgg atcacgcaag aaagccgaga
     7681 tttagccaca taaccgcccg ccggctgcag caatcgtggc tgttaagcgt tccatcgccg
     7741 gtcccacccg tcccgaggcc atccctaatg aaacgtcact tttttctcgc agggctcgcc
     7801 gccatcgtgg cgttgccgtt tccacaagcc gctttagcgc agcgcgcgca atatgaagcg
     7861 ctgatcgcca gccacgccca ggccaacggg gtgccggagg ccctggtgca tcgcgtgatc
     7921 gtgcgtgaaa gccgctatca gcctcacctg atcggacgag gcggcaccat cggcctgatg
     7981 cagatcaagc tcgcgaccgc gcgcggcctt ggttacaccg gcacggccga tggcctgcgc
     8041 gatcccaaca ccaatctcac ctatgccgta aaatatctcg ccggcgccta ccgcgctgcg
     8101 aacggcgacc acaaccgcgc cgtcgcctat tacgccagcg gctattacta cgccgccaaa
     8161 cgccagcggc acgaacatat caggcattcc gaaccgatgc tcgcgagcgc acccgcgaaa
     8221 tagcgctaga agctgctgat ccggcatcca tcgtttgggg ggatcccggt tctgcggagc
     8281 agcgtaagaa cgctgcaccg cgcccgggaa acggccaaac gcggggcagg cattcagtgc
     8341 ggctaccggt cagatgcgtt gacacttccc accatcggcc tacattagct ctgttccgag
     8401 gggagctccg atgaggagct gagagaccgc acgctcaaat cctgttcgca ggtggcgaga
     8461 ccgcggtgac cctttgaacc tgatccgggt catgccggcg aagggacagg gatgtaccag
     8521 aggccagaat cgtcgcgggg ggaatccccc gtttccgtta tcggcgcagg cattgccggc
     8581 gcatggcagg cactgttgtt tgcgcgggcc ggccaccggg tcacgctgca tgagcgcagc
     8641 gatgcggcga tgacgcagtc gaccagcacc tgggccggcg gcatgctcgc gccgtggtgc
     8701 gaggccgaga catcggagcc cgtaatcagc cggctcggca tccgctcgct cgatctgtgg
     8761 cgcgagcatt ttcccaagac gccgttcaac ggttcgctgg tggtggcgca tccacgcgac
     8821 cgcggcgatt tcgaacgttt tgccaggctc acatcgggcc atcggcggct cgacgcacag
     8881 gctgtgaccg aactcgaacc gtcgctggaa gaccgttttc gcgagggcct gttctatgcc
     8941 ggcgaaggcc atgtcgagcc gcgccgtgtg ttgccggaat tgcacgcgcg cttcaccgcg
     9001 gccggcggca ccatccagtt caacagcgaa gccgacgcgt ccgaactcga cggtatcgtg
     9061 atcgattgcc gcggcctcgc cgggcgcgac acccaaccag aactgcgcgg cgtcaagggc
     9121 gagatgatcg tcgtcgagac cgacgaggtc gaactgtcgc gtccggtgcg gttgatccat
     9181 ccgcgctggc cgctgtacgt gattccgcgc ggcggcaacc gcttcatgct gggcgcgacc
     9241 tcgatcgagg ccgaggacaa tggcgtcagc gtccgctcgg cgctggagct gttgggcgcg
     9301 gcctatgcgg tgcatccggc ctttgcggaa gcgcgcatcg tcgaattcgg ctccgggctg
     9361 cg
//
LOCUS       DASWGM010001018         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_411495,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001018 DASWGM010000000
VERSION     DASWGM010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411495"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VGA15_27445"
     CDS             <1..679
                     /locus_tag="VGA15_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX9591464.1"
                     /translation="AWIVGVGGLFGYHALYFLALRFAPPADAGLLNYLWPLLIVLFSS
                     LLPGERLAPHHIIGALLGLAGTVLLLAGNIGSGFAPGQVPGLIAAFVAAFVWAAYSVM
                     SRKLKSVPTDAVAGFCLATALLAAAVHGLVEVTVWPDTTAQWLAIAALGVGPVGAAFY
                     AWDIGMKRGDIRVLGAASYATPLLSTAFLILAGFAKPSAAIAIAAVLIAGGGLIAAKD
                     MMVKRSA"
     gene            complement(819..1514)
                     /locus_tag="VGA15_27450"
     CDS             complement(819..1514)
                     /locus_tag="VGA15_27450"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX9591465.1"
                     /translation="MLSTIDITDIAGSQVIEPAILYFGTPVVLIGSTNEDGSANLAPM
                     SSAWWVGWRCMLGLASNSKTTENMIRTGECVLNLPSADLADAVNNLARATGSNPVPAG
                     KIRHGYRHEKDKFEISGLTRLAGETVAAPRAAECPVQIEAKIAQVHEMAQEDVVWRGN
                     LVAIEVRVTRVHAHPGIMMDRQPHRIDPDKWRPLIMSFQQFYGLTPERLQRSELGQIP
                     ESAYKPPRWRPAA"
     gene            complement(1590..2024)
                     /locus_tag="VGA15_27455"
     CDS             complement(1590..2024)
                     /locus_tag="VGA15_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX9591466.1"
                     /translation="MVRTPPAADIIARLELKPHPEGGHYRETFRDPRVDANGRSVSTA
                     IYFLLARGERSHWHRIDAVEAWHYYAGSALVLHIADDRGQRIVRLGPDLAAGEVPQAM
                     VPAQAWQAAESSGDWTLVGCTVAPGFEFAAFELAEPDWFPGR"
     gene            complement(2024..2791)
                     /gene="gloB"
                     /locus_tag="VGA15_27460"
     CDS             complement(2024..2791)
                     /gene="gloB"
                     /locus_tag="VGA15_27460"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX9591467.1"
                     /translation="MAAEIRLFPCLTDNFGYLIHDSATGATASIDAPEAAPIVKALER
                     EGWTLTDILVTHHHGDHVGGIPELKQKFGCRVVAPHDKSAKIANVDLRVGQGDVVKIG
                     GLLARVLETPGHTLDHISYVFDSEKALFAADTLFSIGCGRVFEGNYPMMWDSLLKLRA
                     LPDDFKLYCGHEYTAANVKFALTVEPDNAALKARAAEVTRLRADGKPTVPTLLGEEKK
                     TNVFLRADEPSVAASVRMKDRSAAEVFGELRERKNKS"
     gene            2924..3667
                     /locus_tag="VGA15_27465"
     CDS             2924..3667
                     /locus_tag="VGA15_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX9591468.1"
                     /translation="MTIDVIDLRDFYSQRLGIVARRLINRGIHARWPNAEGQRVLGLG
                     YPTPYLGLFREDSERCIAFMPAAQGVLKWPTARPALATLVDEFSLPLSDAAVDRILMV
                     HSLEMSDDPEGLLREMWRVLAPSGRLIAVIPNRRGVWTRTDTTPFGHGRPYSRSQITQ
                     LLRQTWFTPTAWGEALFMPPVGGGWFLRSAMAWERIGAVLSLPFAGVHIVEATKQVYR
                     AIPALRERTRLIPALQPVLVPSSLQRDDV"
     gene            complement(3826..>4488)
                     /locus_tag="VGA15_27470"
     CDS             complement(3826..>4488)
                     /locus_tag="VGA15_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEX9591469.1"
                     /translation="NNNNRRGQNPMTRVFESNGPDIKIRGTASHVAEKYVQLARDARS
                     SGDPVAAENYYQHAEHYFRLIAAAQEQFRQNQPQPRTENEMPSEDGDDESESFSNFGQ
                     EPGFVPAQPQPFVPRDNNPREAQPYQPREQHPPREHRPQPQFQPPQPQPQPVIPDSVS
                     VDRLPSFITGPQPQINGGPGGFEGNGGERFPRRRRRPHGPRPEGLPAPVTPGDDFNPG
                     NE"
BASE COUNT          716 a         1393 c         1489 g          890 t
ORIGIN      
        1 tgcatggatc gtcggagtcg gcgggttgtt cggataccac gccctgtatt ttctggcgct
       61 gcgcttcgcg cctccggccg acgcaggctt gcttaattat ctctggccgc tgttgatcgt
      121 gctgttttcg tcgctgctgc cgggcgagcg gctggcgccg caccacatca tcggggcact
      181 gctcgggctc gccggaacgg tgctgctgct ggccggtaac atcggcagcg gctttgcgcc
      241 gggacaagtg cctgggctga tcgcggcgtt tgtcgctgcc ttcgtgtggg cggcctattc
      301 ggtgatgtcg cgcaagctca aatcggtgcc aacggacgcg gtggcgggct tttgtctcgc
      361 cacggcgctg ctcgcagcgg cggtgcatgg gctggtcgag gtcacggtct ggcccgatac
      421 tacggcgcaa tggctggcga tcgcggcgct cggtgtcgga ccggtcggag cagcgtttta
      481 tgcctgggat atcggaatga agcgcggcga catccgcgtg ctgggtgctg cgtcctacgc
      541 gacgccgcta ctctcgaccg cgtttttgat tctggcgggc tttgcaaagc cgagtgccgc
      601 gatcgccatc gccgcggtgc tgatcgcggg cggcggattg attgcggcga aggatatgat
      661 ggtgaagcga tcagcttagg tcggttggct catcaaccat aatagccgtc attccggggc
      721 gcgagtgaag cgagcgagcc cggaatccat aacccctgcg gggagtatgg attccgggcc
      781 tacgccaaga ggcgcatccc ggaatgacaa gcggctcgtc aagccgccgg ccgccagcgt
      841 ggtggcttgt aggcgctctc cggaatttgt ccgagttcgg agcgctgcag ccgttcgggc
      901 gtgaggccgt agaactgctg aaagctcatg atcagcggcc gccatttgtc gggatcgatg
      961 cgatggggtt ggcggtccat catgattccc ggatgcgcgt gcacgcgggt gacgcgaacc
     1021 tcgatcgcca cgagattgcc gcgccagact acgtcctcct gcgccatctc atggacctgc
     1081 gcgatctttg cctcgatctg caccgggcat tccgcggcgc gcggcgcggc aaccgtctcg
     1141 cctgccaatc gcgtcaggcc gctgatctcg aatttgtcct tctcgtgccg gtagccgtgc
     1201 ctgatttttc ccgcgggcac gggattcgag ccggtggcgc gcgccagatt gttgacggca
     1261 tcggcgagat ccgccgaggg aaggttaagt acgcactcgc cggttcgaat catgttttcg
     1321 gtggttttgg agttgcttgc cagtcccagc atgcagcgcc agccgaccca ccaggccgac
     1381 gacatcggcg cgaggttggc ggaaccgtct tcgttggtcg agccgatcag gaccactggc
     1441 gtcccgaaat acagaatcgc cggctcgatg acctgcgaac ccgcaatatc ggtgatatcg
     1501 attgtgctga gcatgtcggc ttcctcatgt gaagccttaa acgtagtgag ttggtgcgga
     1561 cacacccacc cgattcccga atgcattttt cagcggccgg gaaaccaatc aggctcagcc
     1621 aattcgaaag ccgcaaactc gaatcccggc gcgacggtgc atccgaccag cgtccagtcg
     1681 ccgctgctct cggcggcctg ccaggcctgc gccggcacca ttgcctgcgg tacttcgccc
     1741 gcggcgagat cgggccctaa cctgacgatg cgctggccgc gatcatcggc aatgtgcaag
     1801 acaagcgcgc tgccggcgta ataatgccag gcctcgaccg catcgatacg gtgccagtgc
     1861 gagcgttccc cgcgcgccag caggaaatag atcgcggtgg agaccgagcg tccgttggca
     1921 tcgacgcgcg gatcgcgaaa ggtctcgcga taatgcccac cctccgggtg cggcttcaac
     1981 tcgagccgcg caatgatgtc ggcggcggga ggcgtccgga ccatcaggac ttgttcttgc
     2041 gttcgcgcaa ttcgccgaac acctcggcag cgctcctgtc cttcatccgc acgctggccg
     2101 cgaccgacgg ttcgtcggcg cgcaggaaca cattggtctt cttttcttcg ccaagcagcg
     2161 tcgggacagt cggcttgccg tctgcgcgca accgcgtcac ttccgccgcg cgtgctttca
     2221 gcgcggcatt gtccggctcg acggtcagcg caaacttgac gttggccgcg gtatattcgt
     2281 ggccgcaata gagtttgaaa tcatcgggca gggcgcgcaa cttcaagagc gagtcccaca
     2341 tcatcggata gttaccttcg aacacccgcc cgcagccgat cgagaacagt gtgtcggcgg
     2401 cgaacagcgc cttctcgctg tcgaacacgt agctgatgtg atcgagggtg tggcccggtg
     2461 tctccagcac tcgcgccagg aggcctccga tcttcacgac atcgccctga ccgacgcgca
     2521 gatcgacgtt ggcaatcttg gccgacttgt cgtggggcgc gaccacgcgg catccgaatt
     2581 tctgtttcag ctcgggaatg ccgccgacat ggtcgccgtg atgatgggtg accagaatat
     2641 ccgtcagcgt ccaaccctcg cgctccagcg ccttgacgat gggcgcggct tccggagcgt
     2701 cgatcgaggc ggtcgccccg gtcgcggaat catggatcag gtaaccgaaa ttgtcggtca
     2761 ggcaggggaa caagcgaatt tcggcagcca tgatatctcc atgttccaga taagcggcga
     2821 ggctagcaca gccatacatg gcggggaaat atggtgttaa cgctcaccgg gcaatgcgtg
     2881 attcggcgcc ccgcgcgacg cggcgacgtg ttagataact ttcatgacca tcgatgtcat
     2941 cgatctccgc gacttctatt cgcagcgcct cggcatcgtg gcgcggcggc tgatcaatcg
     3001 tggcattcac gcgcgctggc ccaacgcgga aggccagcgg gtactcggcc tcggctatcc
     3061 cacgccctat ctcgggcttt ttcgcgaaga tagcgagcgt tgcatcgcct tcatgccggc
     3121 ggcgcagggc gtgctgaaat ggccgaccgc gcggcccgcg ctggcaaccc tggtcgatga
     3181 attttcgctg ccgctatcgg acgccgccgt cgaccgcatc ctgatggttc actctctgga
     3241 aatgtccgac gatcccgaag ggctgctgcg cgagatgtgg cgcgtgctgg caccctctgg
     3301 ccgattgatt gctgtcattc cgaaccggcg cggggtgtgg acgcgcaccg acaccacgcc
     3361 gtttggccac ggccggccct attcgcgctc gcagatcacg caattgctgc ggcagacctg
     3421 gttcacgccg acggcgtggg gcgaggcgct gttcatgccg ccggtcgggg gcggctggtt
     3481 cctgcgctcc gcgatggcat gggagcgcat cggggctgtg ctatcgctgc cgttcgccgg
     3541 cgttcatatc gtggaagcca ccaagcaggt ctaccgggcg atcccggcgc tccgcgaacg
     3601 cacgcgtctc attccggcgc tgcagccggt gctggtgccg tcgtcgctgc agcgggatga
     3661 tgtttaactt ttcacacgtc gtcgcggcga acgccgggac ccataatccc tggcgttgat
     3721 tgtggaagga aggcgtctac tccagcgtct aaacgagacg gcacagagta tgggtcccgg
     3781 cggttcgccg ggacgacgcg ggagagtgcg acgctgagcg ttgtcttact cattccccgg
     3841 attgaaatcg tcacccggcg tcaccggcgc gggcaggcct tcggggcgcg ggccatgcgg
     3901 ccggcgccgc cgccgcggga aacgctcgcc gccattgccc tcgaaaccgc cagggccgcc
     3961 gttgatttgc ggctgcggtc cggtgatgaa cgatggcagg cgatcgacgc tgacgctgtc
     4021 gggaatcacc ggctgtggct ggggctgcgg cggttgaaac tgcggctgcg ggcgatgctc
     4081 gcggggcgga tgctgctcgc gcggctggta gggctgggcc tcgcgcggat tgttgtcgcg
     4141 cggcacgaac ggctgcggct gcgccgggac gaagccgggc tcctgcccga aattcgaaaa
     4201 gctttcgctc tcgtcgtcgc cgtcctccga aggcatttcg ttctcggtgc gcggctgcgg
     4261 ctgattctga cggaactgtt cctgggcagc ggcgatcagg cggaaataat gttcggcatg
     4321 ctggtagtaa ttctcggcgg ccaccggatc gccggaagag cgtgcgtcgc gcgccagctg
     4381 cacgtatttc tcggcgacgt gggatgcggt gccgcggatc ttgatgtcgg gtccgttcga
     4441 ctcgaacacc cgcgtcatcg ggttctggcc gcgccggttg ttgttgtt
//
LOCUS       DASWGM010001019         4650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1931021,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001019 DASWGM010000000
VERSION     DASWGM010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931021"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VGA15_27475"
     CDS             <1..354
                     /locus_tag="VGA15_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX9591470.1"
                     /translation="KGAKGEAWTGVRNFTARQNLVKMKKGDRAFFYHSNEGKEIVGIA
                     EVIKEAYPDPTDKTGKFVCVDIKADEPLKTPVTMAAIKADKRLTGMALVKYSRLSVQP
                     VTAEEWKIVCKMGGM"
     gene            complement(401..1504)
                     /locus_tag="VGA15_27480"
     CDS             complement(401..1504)
                     /locus_tag="VGA15_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX9591471.1"
                     /translation="MMDQQNFVAARPVSADPHVAVTPGHPASVESLARELGLKLGDQN
                     QLTIRRVKRGKNYSFVRANGTPIRHTGTIKRLHSMAVPPAYAEVRYAPDPTSHLQAVG
                     RDAAGRLQYRYHSDWEKVREHRKAHRLARLVAALPKIRRNVSQHLKGDAPTREFALSA
                     VIELIARTAIRPGNESYARLNGTRGATTLLKSNVVLEDDSVVLTFKAKGGKAVRKECD
                     AAKLVRAIGILRGVPGKRMFQYRDNSGNVRAVSTTQVNAFLREIAGIKISLKDFRTLM
                     ASAVVLESLSRISPAASARGRRKQVLEAVRAAADELSNTPAICRKSYVHDTIVTAFED
                     GILERFAATMKGYRSQSKREQLLAQVVSAAAAA"
     gene            1916..3868
                     /gene="acs"
                     /locus_tag="VGA15_27485"
     CDS             1916..3868
                     /gene="acs"
                     /locus_tag="VGA15_27485"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX9591472.1"
                     /translation="MSEKIYDVSAEWAKRAWVDQAKYRQMYARSVSDPNGFWAEQGKR
                     IGWIKPFTKVENASFAPGNVSIKWFEDGVLNVAWNCIDRHLDKRGDQTAIIWEGDNPS
                     ESKHITYRQLHDEVCKFANILRTRNVKKGDRVTIYLPMIPEAAYAMLACARIGAIHSV
                     VFAGFSPDSLAQRITDCQSKIVITADEGLRGGKKVPLKANVDAAIAKSGGVDWVIVVK
                     RTGAAVDMNPSRDFWYHDAAKMVTTECPCEHMHAEDPLFILYTSGSTGQPKGVLHTSG
                     GYLVFASMTHQYVFDYHDGDIFWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPNYP
                     DNSRFWNVIDKHKVNIFYTAPTAIRALMQGGDEPVKKTSRKSLRLLGTVGEPINPEAW
                     EWYHRVVGDERCPIVDTWWQTETGGILITPLPGATKLKPGSATEPFFGVQPEIVDADG
                     NVLGGECQGNLCIAKSWPGLMRTVYGDHARFEQTYFSTYKGKYFTGDGCRRDADGYYW
                     ITGRVDDVINVSGHRMGTAEVESSLVAHAKVSEAAVVGYPHNIKGQGIYAYVTLMDGA
                     EPSEELRKELVAWVRKDIGPIASPDQIQFAPGLPKTRSGKIMRRILRKIAEDEPGSLG
                     DTSTLADPAVVDDLVAHRQNKKDAGV"
     gene            4344..>4650
                     /locus_tag="VGA15_27490"
     CDS             4344..>4650
                     /locus_tag="VGA15_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9591473.1"
                     /translation="MSLRIAVALAATIGAGMLMSSEAEARPDMVGFRGDYSPGTIVVK
                     TNERRLYLVLDDGHAVRYPVGVGKAGKQWAGTTRIDGKYLEPAWSPPAEVKHDKPSMP
                     "
BASE COUNT          905 a         1408 c         1487 g          850 t
ORIGIN      
        1 aaaggcgcca agggcgaagc ctggaccggc gtgcgcaatt tcacagcccg acagaacctc
       61 gtgaagatga agaagggcga ccgcgccttc ttctatcatt ccaacgaggg caaggagatc
      121 gtcggcatcg cggaagttat caaggaggcc tatcccgatc ccaccgacaa gaccggcaaa
      181 ttcgtctgcg tcgacatcaa agccgacgaa cccctgaaga cgccggtgac gatggccgcg
      241 atcaaggccg acaagcggct gaccggcatg gcgctggtga aatattcaag gctgtcggtg
      301 cagccggtga cggcggaaga gtggaagatc gtctgcaaga tggggggaat gtagctctta
      361 cttcccctct ccccttgtgg gagagggaag acgaagaaag ctacgccgcc gctgccgctg
      421 acaccacctg cgccaaaagc tgctcgcgct tggactgcga gcgatagcct ttcatggtcg
      481 cggcgaaacg ctcgaggatg ccgtcttcga acgcggtgac gatggtgtcg tggacgtagc
      541 tcttgcggca gatcgccggc gtgttcgaca gctcatccgc cgcggcgcgg acggcctcca
      601 gcacctgttt gcgacggccg cgggcgctgg ccgccggcga aatccgcgac agcgattcca
      661 gcaccacggc ggacgccatc agggtacgaa aatccttcag cgatatcttg atgccggcga
      721 tctcgcgcag gaacgcgttg acctgcgtgg tagagaccgc gcggacattg ccggaattgt
      781 cccggtactg aaacatccgc ttgcccggca cgccgcgaag gatgccgatg gcgcgcacca
      841 gcttggcggc gtcgcattcc ttgcgcacgg ccttgccgcc cttggccttg aaggtcagca
      901 cgacggagtc gtcttccagc acgacattgg atttcagcag cgtggtggcg ccgcgggtgc
      961 cgttgaggcg ggcgtaggat tcgttgcccg gacggatcgc ggtgcgcgcg atcagttcga
     1021 tcaccgccga cagcgcgaat tcacgggtcg gggcgtcgcc cttgagatgc tgcgagacgt
     1081 tgcggcgaat tttcggcagg gccgcgacca gacgcgccag ccgatgcgcc ttgcggtgct
     1141 cgcggacctt ttcccaatcg gaatggtagc ggtattgcag ccggcccgcg gcatcgcggc
     1201 cgacggcctg caggtgcgag gtcggatcgg gggcgtagcg cacctcggca taggccgggg
     1261 gcaccgccat cgagtggaga cgcttgatgg tgccggtgtg gcggatcggc gttccgttgg
     1321 cgcgaacgaa cgaatagttc ttgccgcgct tgacgcggcg gatggtcaat tggttctggt
     1381 cgccgagctt caaacccagt tcccgggcga gcgattcgac gcttgccggg tgtcccggcg
     1441 tcacagccac atgcggatcg gcggaaacgg gccgcgcagc cacgaaattc tgctgatcca
     1501 tcatgacctt agacgccttg ctccgaatgt tctcacggta atgccgattc ttgatcctta
     1561 gttccggcag accgcctgac ctaggggagg ccatttaggg ggtaattgtc ttaagcgcca
     1621 gcccaagaag tgagggcgac ggtttccaga tacctcccgg caggaaagct agcccgtttg
     1681 gggatttttt ggttatcgga aaacgcgatc gtgatggcgc tggatgctgg gcggatgccg
     1741 gggcgacccc cgccgtctgg cccaacggtt tgatcctgcg catggcgagg agcgtcgcca
     1801 aaggtactat ttgttccgct tgtcggcgcc atcaggcccg acgcataatc gttataacaa
     1861 tcaaagacgg cttgcagtgt ccgcgttcac tggccgaatg ggtggagggt caaaaatgtc
     1921 cgagaaaatt tacgacgtat ccgcggagtg ggccaagcgt gcatgggtcg accaggccaa
     1981 ataccgccag atgtatgcgc gctcggtcag cgacccgaac ggcttctggg ccgaacaggg
     2041 caagcgcatc ggctggatca aaccattcac caaggtcgag aacgcgtcct tcgctcccgg
     2101 caacgtctcg atcaaatggt tcgaggacgg cgtcctcaac gtcgcctgga attgcatcga
     2161 ccggcatctc gacaagcgcg gcgaccagac cgccatcatc tgggagggcg acaatccctc
     2221 ggaatcgaag cacatcacct atcggcagct gcacgacgag gtctgcaaat tcgccaacat
     2281 tctgcgcacc cgcaacgtca agaagggcga ccgcgtcacg atctatcttc cgatgattcc
     2341 ggaagccgcc tacgcgatgc tggcctgtgc gcggatcggc gccattcatt cggtggtgtt
     2401 tgccggcttt tcgcccgaca gcctcgccca gcgcatcacc gactgccagt ccaaaatcgt
     2461 catcactgcc gacgagggac tgcgcggcgg caagaaagtc ccgctgaagg ccaatgtcga
     2521 tgccgccatc gccaaaagcg gcggcgtcga ctgggtcatc gtggtcaagc gtaccggcgc
     2581 cgcagtcgac atgaatccgt cgcgcgattt ctggtatcac gacgcggcca agatggtgac
     2641 gacggaatgt ccgtgcgagc atatgcacgc cgaggacccg ctgttcatcc tctatacgtc
     2701 gggttcgacc ggccagccca agggcgtgct gcacaccagc ggcggctatc tggtgttcgc
     2761 ctcgatgacg catcaatacg tgttcgacta tcacgacggc gacatcttct ggtgcaccgc
     2821 cgacgtcggc tgggtcaccg gccacagcta cattgtttat gggcctttgg cgaacggcgc
     2881 gaccacgctg atgttcgaag gcgtgccgaa ttatcccgat aattcgcggt tctggaacgt
     2941 gatcgacaag cacaaggtca acatcttcta caccgcgccg accgcgatcc gcgcgctgat
     3001 gcagggcggc gatgagccgg tcaagaaaac ctcgcgcaag agtcttcgat tgctcggcac
     3061 cgtcggcgag ccgatcaatc cggaagcgtg ggagtggtat catcgcgtgg tcggcgacga
     3121 gcgctgcccg atcgtcgata cttggtggca gacggagacc ggcggcattc tgatcacgcc
     3181 gctgcctggt gcgaccaaac tcaagccggg ttcggcgacc gagccgttct tcggtgtgca
     3241 gcctgagatc gttgatgccg acggcaatgt tctgggcggc gaatgccagg gcaatctgtg
     3301 catcgccaaa tcctggccgg ggctgatgcg cacggtctat ggcgaccatg ctcgcttcga
     3361 acagacctat ttctcgactt acaaaggcaa gtattttacc ggcgacggct gccgccggga
     3421 tgccgacggc tattactgga tcaccggccg cgtcgacgac gtgatcaacg tttccggcca
     3481 tcgcatgggc accgcggagg tcgagagttc gctggtggcg catgccaaag tgtcggaggc
     3541 cgccgtggtc ggctatcccc acaacatcaa gggccagggc atctatgcct atgtcacgct
     3601 gatggatggt gccgagccca gcgaagaatt gcgcaaggaa ctggtggcgt gggtgcgcaa
     3661 ggacatcggt ccgatcgcgt ctcccgacca gatccagttc gcaccgggat tgccaaagac
     3721 ccgatcgggc aagatcatgc gccgcatcct gcgcaagatc gccgaggacg aaccgggcag
     3781 tctcggcgat acctcgacac tggcggatcc ggccgtggtc gacgatctgg tcgcacaccg
     3841 ccagaacaag aaggacgcgg gggtttagaa acaaggctcc acgcgcgggt gccgtgcccc
     3901 ggatgctgcg caacgcaatc agcgcgttca cgcgtgtctt cgacgcgcta cgccttgcgg
     3961 cgtggtgcgc tgccggggtc cacatcgtac acgtcgtcgt cttgggattc gtggatcccg
     4021 gctctgcgtc gcgtcacttc gtgccgcgcc gcgtccggga cacggacagg tgcccccaca
     4081 cacactcacg cgatcgtcag cggcaagctg cccgcggcgg cacatttccc aaagagtgtt
     4141 gttttcaagt catttacttt gtttcgaaca tttcctctct tgcccccgac taaatgagtc
     4201 ccaaggccag cgtggcaacc tgtcagttaa cgcgagcggt taaaaatgcg tcggcaaaac
     4261 cggctgcaca ccggcgcatt acgggctttg catggaattt gacggccccg aacggggtgc
     4321 ttgaagcgaa agtggagaga tcgatgtcgt tgaggatcgc ggtggcgctc gcagccacca
     4381 tcggggcggg gatgttgatg tcgagcgagg ccgaagcgcg gccggacatg gtcggcttcc
     4441 gcggcgacta ttcgccgggg acgatcgtgg tgaagaccaa tgagcgccgg ctctatctcg
     4501 tgctcgatga cggccacgcc gtgcgctatc ccgtcggcgt cggcaaggcc ggcaagcaat
     4561 gggccggcac cacccggatc gacggcaagt atctcgaacc cgcatggtcg ccgccggctg
     4621 aagtgaagca tgacaagccg tcgatgccgg
//
LOCUS       DASWGM010001020         5802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_412034,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001020 DASWGM010000000
VERSION     DASWGM010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412034"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VGA15_27495"
     CDS             <1..462
                     /locus_tag="VGA15_27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591474.1"
                     /translation="FSGQSIAVYAITIAFIIGLWLFFGFTLTGKALRATAVNRLGARL
                     VGIRTTLSGQIAFLLASVIGAISGILIVPITTLYYDTGFLIGLKGFIAAIIGGLVSYP
                     LTAIAALVVGSVEAFSSFYASNFKEVIVFFLILPVLVLRSLTAPAVEEEKD"
     gene            468..2237
                     /locus_tag="VGA15_27500"
     CDS             468..2237
                     /locus_tag="VGA15_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX9591475.1"
                     /translation="MMQRLPIIVFAAVMAAIPFIPGMPPFWIVLLDNIGLAALVAMGL
                     VLLTGVGGLTSFGQAAFCGFGAYTTAVLTTAYGVSPWLTLPLSLLVSGIAAVVLGLVT
                     VRLSGHYLPLGTIAWGIGLFYLFSKLEFLGRNDGISGIPPLTIGSLRMIDPGTIYFAI
                     WIAVLISALLTMNLLDSRTGRAIRALRRGHVAAEAFGVQTPRAKLLVFIYAAVLAGLS
                     GWLYAHFQRAANPTPFGAQAGIEYLFIAVVGGAGYVWGGVLGAAIVVILKEILQSYLP
                     YLFHGEGQLETIVFGILLVLLLQLAPTGVWPWLMARLPFKPNRKRPDTSLALPARIRT
                     AAVPAALLQIDNARKQFGGVIAVNDVSFDVEAREIVALIGPNGAGKSTTFNLITGVLT
                     SSGGTISVLGKNIDNAPPQEVAKLGVARTFQHVKLVPDMSVLENVAIGTHLRGHAGAL
                     SSMFRLDRADEAKLLAEAARQIERVGLGDQIDALAGSLSLGQQRIVEIARALCVDPIL
                     LLLDEPAAGLRHMEKQRLAGLLRQLRDGGMSVLLVEHDMGFVMDLADRIVVLDFGTKI
                     AEGTPEAIKSNPEVIKAYLGAVA"
     gene            2234..2965
                     /locus_tag="VGA15_27505"
     CDS             2234..2965
                     /locus_tag="VGA15_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591476.1"
                     /translation="MSALLSVSSAHVAYGKVEAVRSVSLEVADKEIVTIIGANGAGKT
                     TLLNAIMGILPLKGRVTFAGADVLRLEIEDRVAAGLCLVPEHRELFGTMNVEDNLQLG
                     AFRIAKAATAVAFERVYTLFPRLKERRKQLAGTLSGGEQQMLAMGRALMGAPKLLMLD
                     EPSLGLAPIIVADIFRTIGELRASGVSVLLVEQNAQAALQIADRAYVMELGEFILNGP
                     AQDIATNQRVAASYLGFAHEGASEI"
     gene            complement(3096..4250)
                     /locus_tag="VGA15_27510"
     CDS             complement(3096..4250)
                     /locus_tag="VGA15_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591477.1"
                     /translation="MKKFYLSAAVVAASLALPGLPAMAQTNEIVIGITVTTTGPAAAL
                     GIPERNALEFVPKEIGGVPLKVIVLDDGGDPTNATTNARRFVTESKADIIMGSSTTPP
                     SVAVSNVANEAGIPHFGLAPFPITPERAKWSVDMPQPVPIMGKVLYEHMKAHNIKTVG
                     YIGYSDSYGDLWFNDFKNQGIPIGMTLVDEERFARPDTSVAGQVLKLVAANPDAVLVG
                     ASGTAAGLPQAALRERGYKGLIYQTHGAASMDFIRIAGPAAEGVIMASGPVMNPEGQP
                     DTALTKKPGLALNAAYEAKYGPNSRSQFAGHSYDAFEVLKRVIPVALKAGKPGTPEFR
                     EGIRQAWLTEREIVASQGVYNFTEKDRYGLDDRSRIILTVKNGKYVAAGE"
     gene            complement(4353..4772)
                     /locus_tag="VGA15_27515"
     CDS             complement(4353..4772)
                     /locus_tag="VGA15_27515"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX9591478.1"
                     /translation="MFVHRRDVQIQWGDCDPANIVYYPRYFEMFDDSTSIMFEAAGFS
                     KQDIIKKYGLVGIPMVDTRAKFYVASTHGDWITIESRIESFKRSSFEVTHKVFKGEAL
                     AIEAFETRVLVGRHPDDPARLKSAAVPEEIIARFMRG"
     gene            complement(4783..5544)
                     /locus_tag="VGA15_27520"
     CDS             complement(4783..5544)
                     /locus_tag="VGA15_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9591479.1"
                     /translation="MQLKDQAAIVTGGASGLGAATARRLAAQGAKVAVCDLNAKLAES
                     VAAEIGGVAVVCDVADASSAEAAVAAASKAHGPARVLINCAGIGVAKRVIGREGPMPL
                     ADFEKVIKVNLIGSFNMLRLATAEMSKLEPLETGERGVVISTASVAAYDGQIGQSAYS
                     ASKGGIVAMTLPIARELAQFGIRVLTIAPGLFLTPLLANLPQEAQDSLAAAIPFPRRL
                     GHADEFASLALHMIDNPYLNGEVVRLDASLRMAPR"
     gene            complement(5584..>5802)
                     /locus_tag="VGA15_27525"
     CDS             complement(5584..>5802)
                     /locus_tag="VGA15_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643308.1"
                     /note="catalyzes the conversion of ferulic acid to
                     feruloyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEX9591480.1"
                     /translation="AFRERLIKFLATATGSSTRITRAILLDTPLSIDRGEVTDKGSIN
                     QRAVLDARAALIEELYSPTPATHMMTLG"
BASE COUNT          982 a         1899 c         1791 g         1130 t
ORIGIN      
        1 ttcagcggcc agagcatcgc ggtctacgcc atcaccattg ccttcattat cggcctgtgg
       61 ctgttcttcg gttttaccct caccggaaag gcgctgcgcg ccaccgccgt caatcgcctg
      121 ggcgcgcgcc tggtcggcat ccgaaccacg ctgtcgggac agatcgcgtt cctgctggcc
      181 tccgtgatcg gggcgatctc gggaatcctg atcgtgccga tcacgacgct gtattacgac
      241 accggcttcc tgatcggcct caagggattt atcgccgcca tcattggcgg cctcgtgagc
      301 taccccttga ccgcgatcgc ggccttggtg gtcggatcgg tcgaggcatt ctcctcgttc
      361 tatgccagca atttcaagga ggtcatcgtc tttttcctga tccttccggt tctcgtattg
      421 cggtcgctga cagcgcccgc ggtcgaggaa gagaaggatt gaccgcgatg atgcagcggc
      481 tgcccatcat cgtcttcgcc gctgtgatgg cggcgattcc gttcatcccg ggcatgccgc
      541 cattctggat cgtgctcttg gacaatatcg ggctcgcagc acttgtggcg atggggcttg
      601 tgctcctgac cggcgtcggc ggcctcacct cgttcggcca ggccgcgttt tgcggttttg
      661 gcgcctatac aacggcggtg cttaccaccg cctatggcgt gtcaccctgg ctgacattgc
      721 cgctctcgct gctggtctca ggcatcgccg cggtcgtgct cggcctcgtc acggtgcggc
      781 tgtcgggaca ttatctgccg ctcggcacca tcgcctgggg catcggcctg ttctatctgt
      841 tcagcaagct cgaattcctc ggccgcaatg acggcatctc cggcattccg ccgctgacga
      901 tcgggtcgct cagaatgatc gaccccggca cgatttattt tgcgatctgg atcgcggtgc
      961 tgatctcggc gctgctcacc atgaaccttc tggactcgcg caccggccgc gccatccgcg
     1021 cgctgcggcg cggccacgtc gccgccgaag ccttcggcgt gcagacgccc cgcgccaagc
     1081 tgctggtttt catctatgcg gcggtgctgg ccggcctgtc cggctggctc tatgcgcatt
     1141 tccaacgcgc cgccaatccg acgccgttcg gcgctcaggc cggcatcgaa tatctcttca
     1201 tcgccgtggt cggcggcgcc ggctatgtct ggggcggcgt gcttggcgct gccatcgtcg
     1261 tcatcctgaa agagattctg cagagctatc tgccatatct gtttcatggc gaaggccagc
     1321 tcgagaccat cgtgttcggc atcctgctgg tgctgctgct gcaactggca ccgaccggcg
     1381 tctggccctg gctgatggcg cgattgccgt tcaagccgaa ccgcaagcgg cccgatacct
     1441 ccctggcctt gccggcgcgc atcagaaccg cggccgttcc tgccgcgctg ctgcagatcg
     1501 acaacgcgcg caagcagttc ggcggcgtga ttgccgtcaa cgacgtctcc ttcgacgtcg
     1561 aggcccgcga gatcgtcgcg ctgatcggcc ccaatggcgc cggcaagagc acgaccttca
     1621 acctgatcac cggcgtgctc acgtcatccg gcggcaccat ctccgtgctc ggcaagaaca
     1681 tcgacaacgc accgccgcag gaggtcgcaa aacttggcgt cgcccgcacc ttccagcatg
     1741 tcaaactggt gccggatatg agcgtgctgg aaaacgtcgc gattggcacg catctgcgtg
     1801 gccatgcagg cgccctctcc agcatgttcc gcctcgaccg tgctgacgag gcaaagctgc
     1861 tggcggaagc ggcgcgccag atcgagcgcg tcggcctcgg cgaccagatc gacgccctcg
     1921 ccggcagcct gtcgctcggc cagcagcgca tcgttgagat cgcccgcgcg ctgtgcgtcg
     1981 acccgatcct gctgctgctg gacgagccgg ccgcgggatt gcgccatatg gaaaagcaac
     2041 ggctcgcagg cctgctgcgt caactgcgcg acggcggcat gtcggtgctg ctggtggaac
     2101 acgacatggg atttgtgatg gatctggccg accgcatcgt ggtgctcgac ttcggcacca
     2161 agatcgccga aggcacgccg gaagcgatca agtccaatcc cgaggtgatc aaggcctacc
     2221 tcggagctgt cgcatgagcg cgctattgtc ggtgtcatca gcgcacgtcg cctatggcaa
     2281 ggtcgaagcg gtccgctcgg tgtcgctcga ggtcgccgac aaggagatcg tcaccatcat
     2341 cggcgccaac ggcgccggca agaccacgct gttgaatgcc atcatgggga tcctgccgct
     2401 gaagggccgt gtgaccttcg ccggcgcgga cgtgttgcgg cttgagatcg aggaccgcgt
     2461 cgcggcgggt ctttgtctcg tgccggagca tcgcgagctg ttcggcacca tgaatgtcga
     2521 ggacaatctg cagctcggcg cgttccggat cgccaaggcg gccacggcgg tcgcgttcga
     2581 acgggtctac acgctgtttc cgcggctgaa ggagcggcgc aagcaactgg ccgggacgct
     2641 gtcgggcggc gagcagcaga tgctggcgat gggccgcgcc ttgatgggcg cgccaaagct
     2701 gttgatgctg gacgagccga gccttgggct ggcgccgatc atcgtcgccg atattttccg
     2761 gaccatcggc gaattgcgcg cgagcggcgt ctcggtgctg ctggtcgagc agaacgcgca
     2821 agccgcactg caaatcgccg accgcgccta tgtgatggag ctcggcgaat tcattctgaa
     2881 tggaccggcg caagacatcg caaccaacca gcgcgtcgcc gcgagctatc tcggatttgc
     2941 ccacgagggc gcgagcgaga tttaggtcgt agggtgggca aaggcgctct tcgcgccgtg
     3001 cccaccacta accaccgcaa tggtgggcac gcttcgcgtt gcccacccta cagttccgcg
     3061 ttggcggatg atgacgccag cgcaacgaaa cgcgcctact cgcccgcggc gacgtacttt
     3121 ccattcttca ccgtcaggat gatgcgcgag cgatcgtcca gaccgtagcg gtctttttcg
     3181 gtgaaattat agacgccctg gctcgccacg atttcccgtt cggtcagcca ggcctgacgg
     3241 attccctcgc gaaattccgg cgttcccggc ttacccgcct tcagcgccac cggaatgacg
     3301 cgcttgagga cttcaaaggc atcgtaggaa tgtccggcga actggctgcg gctgttcgga
     3361 ccatacttgg cctcataggc ggcgttgagc gcgaggccgg gcttcttggt cagcgcagtg
     3421 tcgggctggc cttccggatt catcaccgga cctgacgcca tgatcacgcc ttccgccgcg
     3481 ggacctgcga tgcggatgaa gtccatgctg gcggcgccat gagtctggta gatcagaccc
     3541 ttgtagccac gttcgcgcag cgccgcctgc ggcagccccg ccgcggtgcc ggacgcgccg
     3601 accagcaccg catcggggtt ggcggcgacg agtttcagca cctgtcccgc caccgaggta
     3661 tccggccgcg caaagcgttc ttcgtcgacc agcgtcatgc cgatcggaat gccctggttc
     3721 ttgaagtcgt tgaaccagag atcgccgtag ctgtcggaat agccgatata gccgacggtc
     3781 ttgatgttat gcgccttcat gtgctcgtac agcaccttgc ccatgatcgg aaccggctgc
     3841 ggcatatcga ccgaccattt cgcccgttcc ggcgtaatcg gaaacggcgc cagaccgaaa
     3901 tgcggaatgc ccgcctcgtt ggcgacattg gagaccgcca ccgatggcgg cgtggtggac
     3961 gagcccatga tgatgtcggc cttggattcc gtgacgaagc gccgcgcatt ggtggtggca
     4021 ttggtcggat cgccgccgtc gtcgagcaca atgaccttca gcggcacgcc gccgatttcc
     4081 ttcggcacga attcaagcgc attgcgctcg ggaatgccca gcgccgccgc gggtccggtc
     4141 gtcgtgacgg tgatgccgat gacgatctca ttggtctgcg ccattgccgg caaccccggc
     4201 agcgcgagcg acgccgcgac gacagcggcg gacagataga acttcttcat gcatttcctc
     4261 ccttgggcgt tattgcttac aacaaaattt cgattgataa ttcagcccct tctttaggcg
     4321 atgcgcctct ttagggcata cgcccggcca agtcagcccc gcatgaacct cgcaatgatc
     4381 tcttccggca cggctgccga tttcagccgc gccggatcgt ccggatgacg accgaccagc
     4441 acgcgggtct cgaacgcctc gatcgccagc gcctcaccct tgaacacctt gtgggtcacc
     4501 tcaaaactcg aacgcttgaa actctcgatc cggctctcga tcgtgatcca gtcgccatgg
     4561 gtcgacgcga cgtaaaattt cgcccgcgta tccaccatgg gaatgccgac caggccgtat
     4621 ttcttgatga tgtcctgctt ggagaagccg gcggcctcga acatgatcga ggtcgagtcg
     4681 tcgaacattt cgaagtaacg cggataatag acgatgttgg cggggtcgca gtcgccccac
     4741 tggatctgca catcgcgccg atggacgaac atgcgtttat ccctaacgcg gcgccatgcg
     4801 gagcgatgcg tcgagccgca ccacctcgcc gttcagatag ggattgtcga tcatgtgcag
     4861 cgccagcgaa gcgaactcgt cggcatgccc gagccggcgc ggaaacggga tcgccgcggc
     4921 caggctatcc tgcgcttcct gcggcagatt ggcgagcagc ggcgtcagga acaggccggg
     4981 cgcgatggtc agcacgcgaa tgccgaactg cgccaactcg cgcgcgatcg gcaacgtcat
     5041 cgcgacaatg ccgcctttcg aggccgaata ggccgattgt ccgatctggc cgtcataggc
     5101 cgcgaccgac gcggtcgaaa tcacaacacc gcgctcgccg gtttcaagcg gctccagttt
     5161 cgacatttcc gcggtcgcca gccgcaacat gttgaacgag ccgatcagat tgacttttat
     5221 caccttttca aagtcggcga gcggcatcgg gccctcgcgg ccgatcaccc gcttggcgac
     5281 gccgatgccg gcgcaattga tcagcacccg cgccgggcca tgggccttcg acgccgcagc
     5341 gacggcggct tccgccgagg acgcgtcggc aacatcgcag acaaccgcga cgccaccgat
     5401 ctcggccgcg acgctttcgg caagcttggc attgaggtcg cacacggcaa ccttggcacc
     5461 ctgggcagcg agccgacggg ctgtggcggc gcccaatccc gatgcgccgc cggtcacgat
     5521 ggcggcctga tccttcaact gcatggcttc ctcccttgct ccctttggcg aaatggctgt
     5581 ttatcagccg agcgtcatca tgtgggttgc cggcgtcggc gaataaagct cctcgatcag
     5641 cgcggcgcgc gcgtccagca ccgcgcgctg attgatcgag cccttgtcgg taacttcgcc
     5701 gcgatcgatc gacagcggcg tatcgagcag gatcgcgcgg gtaatccgcg tcgatgaccc
     5761 cgtcgcagtc gccagaaatt ttatcagccg ttcgcgaaac gc
//
LOCUS       DASWGM010001021         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1635763,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001021 DASWGM010000000
VERSION     DASWGM010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635763"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VGA15_27530"
     CDS             <1..114
                     /locus_tag="VGA15_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX9591481.1"
                     /translation="EPWTIGTNVSSGCIRMRNEDVIDLYGRVNVGARVVVI"
     gene            complement(187..987)
                     /locus_tag="VGA15_27535"
     CDS             complement(187..987)
                     /locus_tag="VGA15_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HEX9591482.1"
                     /translation="MTPQERQLIDDLFDRLFRVEGAPRDPDAVAAIAQGLRKAPNAVY
                     ALVQTVLLQDEALKRANSRIQELEAGGAGEPQQSGGFLDSMRETIFGQNPSRGSVPNV
                     RPPDMASRPTWNSGQVLQQAQGPGPYNQGPYGAPQAPMGMGGAPMGMGGGGGSFLGTA
                     AAAAAGVVGGSLLLGSIRSMMGGGGHQSFGDSNAFGDKATSQSPWSDQSNSELARDAG
                     INDIASSGDRADDNSRAGLFDSASNDDDHDDMDLDSDDFGGDGDSDYA"
     gene            complement(1113..1958)
                     /locus_tag="VGA15_27540"
     CDS             complement(1113..1958)
                     /locus_tag="VGA15_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX9591483.1"
                     /translation="MNDAAPLRRIWLCADDYGLSPGVNRAIRDLIERGRLNATSAMVV
                     GAAIGRDEVSLLQAAVANSPRCAIGLHVTLTAPFRPLTMHFQPLDGGMFLAFPKLLRA
                     GLLRRLDPEIIHAELMVQFAAFNELFGRAPDFVDGHQHAQLFPVVRDAFLSAVKEAAP
                     NAWVRQGGRDAPLVRRLGTPKALFLDILSAQFRRRAMRANIAFNPGFAGAYDFSQRPD
                     FGVLMRQFLDGLPEGGLIMCHPGFVDETLVSLDPLTVQREHEHAFLGGEHFPDLLAAN
                     KVTLS"
     gene            complement(1955..3001)
                     /locus_tag="VGA15_27545"
     CDS             complement(1955..3001)
                     /locus_tag="VGA15_27545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX9591484.1"
                     /translation="MMLGTDVSGLSTTAATAAAQGLSIVVPLYNEATGLAFLHARLGE
                     MAKALRQRYGLACEVVYVDDGSADATLVIARGLAADALDVQVVSLSRNFGKEAALMAG
                     LDHARRGAILFMDGDGQHPPDIVEKLVGHWIDGGYDVVYTAKAHRDNESLLRRVAVHG
                     FYALINWGARQKIPEDAGDFRLLSPRAAAALRQLPERNRFFKGLASWIGFRQIRVDYE
                     PAARAHGVTTFSPGRLVGLSIEGLTSFSVAPLRFASLLGVLLASAAFLFGLSILWETW
                     MSGKSVPGYPSLVVGLMTIGGVQLIMIGIVGEYIGKILSELKARPIYFVAEHSVKRTE
                     AETGASASERTAAE"
BASE COUNT          616 a          995 c          986 g          565 t
ORIGIN      
        1 gagccctgga ccatcggcac caacgtgtca tccggctgca tccggatgcg caatgaggac
       61 gtgatcgacc tgtatggccg ggtcaatgtc ggcgcgcggg tcgtcgtcat ctgatttttt
      121 cctgaaaacc aaaacggccg ctcgatccgg gcggccgctt tttgtcgttg cgcaagcaaa
      181 aattgatcag gcgtagtcgc tgtccccgtc gccgccgaag tcgtcggaat cgagatccat
      241 gtcgtcatga tcgtcatcgt tcgaggcaga atcgaacaat cctgcgcgcg aattgtcatc
      301 ggcgcgatcg ccggatgaag cgatgtcgtt gatgccggcg tcgcgcgcca attcgctgtt
      361 ggattgatcg ctccacggac tttggcttgt agccttgtcg ccaaacgcgt tgctatcgcc
      421 gaagctctga tggccgccgc ctcccatcat cgagcgaatg ctgccgagca gcagcgatcc
      481 gccgaccacc ccggccgccg cagccgccgc ggttccgaga aacgatccgc cacctccgcc
      541 catgcccata ggtgctccgc ccatgcccat gggtgcctgg ggcgcgccat agggcccttg
      601 attatatggt ccgggacctt gcgcctgctg cagcacctgg ccgctgttcc atgtcgggcg
      661 gctcgccatg tccggcggtc ggacgttcgg caccgagccg cgggatggat tctgcccgaa
      721 tatcgtttcg cgcatcgaat cgaggaaccc gccggattgt tgtggttcgc ccgcgccgcc
      781 ggcctccagt tcctggatgc ggctgttggc gcgcttcagt gcttcgtcct ggagcaggac
      841 cgtttgtacc agcgcgtaaa cggcattggg cgctttgcgc aaaccctggg caatggctgc
      901 aacggcgtcc ggatcgcgcg gggcgccctc caccctgaag agccggtcga aaagatcatc
      961 aatcaattgg cgttcttgcg gtgtcatgga ttctctccct cgctcgcttc gaagcgcgag
     1021 ggagatgtag gggggcttta tgtcgccagt agtggcgccc cgaattaaat ttaggtatgc
     1081 gacaagacgc ccggcgggtt tgttgacgga tgtcaactca atgtaacttt gttcgcagcc
     1141 agcagatccg ggaaatgctc gccgccgagg aaagcgtgtt cgtgttcgcg ttggaccgtc
     1201 agcgggtcga ggctgaccag ggtttcgtcg acgaaccccg ggtgacacat gatgaggcca
     1261 ccctccggca gcccatcgag aaactgccgc atcaagacgc cgaaatcggg ccgctgagaa
     1321 aaatcataag cgccggcgaa tcccggattg aatgcgatat tggcgcgcat ggcgcggcgg
     1381 cgaaattgcg cgctcaggat gtcgagaaat aacgccttgg gcgtgccgag ccgccggacc
     1441 aaaggcgcat cccgtccgcc ctggcgaacc caggcgttgg gcgcggcttc tttcaccgcc
     1501 gaaaggaacg cgtcgcgcac caccggaaac agttgcgcgt gctgatgacc gtcgacgaaa
     1561 tccggggcgc ggccaaatag ttcgttgaaa gccgcgaact gcaccatcag ttccgcatgg
     1621 atgatttcgg gatcgagccg ccgcagcagg cccgcgcgca gcagttttgg aaaggccaag
     1681 aacatgccgc catcgagcgg ctgaaaatgc atcgtcaggg gacgaaacgg cgccgtcaac
     1741 gtgacgtgga ggccgatcgc acagcgggga ctgttggcga cggcggcttg aagcaagctt
     1801 acttcgtcac ggccgatcgc cgcgccgacc accatcgccg atgtggcatt gagacggcca
     1861 cgctcgatca ggtcgcgaat ggcgcggttg acgccgggac tgagaccgta atcgtcggcg
     1921 cacagccaga tacgacgcag cggcgcggcg tcgttcattc ggcggcggtc ctttcgctgg
     1981 cgctcgcgcc ggtttccgcc tcggtgcgtt tgacactgtg ttcagccacg aaatagatcg
     2041 gtcgggcttt caactccgaa agaatcttgc cgatatattc tccgacaatg ccgatcatga
     2101 tgagctgcac gccgccgatc gtcatcaggc cgaccaccag cgagggatag ccgggaaccg
     2161 attttccgct catccaggtt tcccagagga tcgaaaggcc gaacaagaaa gccgctgaag
     2221 cgagcaggac gccgagcagg ctggcaaaac gcagcggcgc gaccgagaac gaggtgaggc
     2281 cttcgatcga aagtccgacc agccggccgg ggctgaacgt ggtgacgccg tgcgcgcggg
     2341 cggcgggttc gtagtcgaca cggatctggc ggaaaccgat ccagctggcg aggcccttga
     2401 agaagcgatt gcgctcggga agctgccgca gcgccgccgc ggcgcgcggc gacagcaggc
     2461 ggaagtcgcc ggcatcttcg gggattttct gccgcgcacc ccaattgatc agcgcgtaga
     2521 agccgtgcac ggccacgcgc cgcagcaacg attcgttgtc gcgatgcgcc ttggcggtat
     2581 agaccacatc atagccgcca tcgatccagt ggccgacaag tttctcgacg atgtcaggcg
     2641 gatgctgacc atcgccgtcc atgaacagga tcgcgccgcg ccgggcatgg tccagtcccg
     2701 ccatcaacgc ggcttccttg ccgaaattgc gcgacagcga caccacctgg acgtcaagcg
     2761 catccgcggc aagcccgcgc gcgatcacga gcgttgcgtc ggcgctgcca tcatcgacat
     2821 agacaacctc gcaggccaag ccgtagcgct gccggagcgc ttttgccatt tcgccaagcc
     2881 gcgcgtgcag aaacgccagc cccgtcgcct cgttatacaa gggcacgacg atcgacagcc
     2941 cctgcgccgc agccgtggcc gcagtggttg ataagccaga aacatccgta cccagcatca
     3001 tcgatcaggt tccaagccgc ccatatgacc gcatatgtta ctcgttgcta gctgtcgcaa
     3061 cgatgaacga aaacaccctc cgtctcactg ccgcaggaat gcctcaagct tggcgaacag
     3121 cgggttgtcg cggtcgagca cgtagtcgag cgacgccacc ga
//
LOCUS       DASWGM010001022         7071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1496379,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001022 DASWGM010000000
VERSION     DASWGM010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496379"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..379
                     /locus_tag="VGA15_27550"
     CDS             101..379
                     /locus_tag="VGA15_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591485.1"
                     /translation="MNVMRVLGLAAVAALLMLTAPAERAQALSLASPGAAAAVQDDTR
                     EMTTEVRWHHHWHHRHWGYHRHWHHRHWHHRHWHRHHWHHRHWHHRYW"
     gene            607..921
                     /locus_tag="VGA15_27555"
     CDS             607..921
                     /locus_tag="VGA15_27555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591486.1"
                     /translation="MKRWIGAAVMAAGLALSGPVATGSVAANSKVGLHKVGLLAVGLH
                     PTDTSKATDLSARRRVRHYRHDAYHPYYRAYYYDRPYDYRPYSYAAPVPFFLGIGFGP
                     RW"
     gene            1189..1752
                     /locus_tag="VGA15_27560"
     CDS             1189..1752
                     /locus_tag="VGA15_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591487.1"
                     /translation="MTRIPDPRRGVRCLVRSLGFAAATTLMLSFATDQRAEALSLISP
                     GAAPAVTHTSDGLTTEVRGGGHGGGGHGGGGHGGGGGHGGGGFHGGGGFHGGGAAFHG
                     GGFRSAHVFHGGGFRHGGFGFRHHRFHRHFYASYYDYPYYYSYHRCRVIWTYYGPRRI
                     CHYRHWHHRHYWHHHYWRHHHRHHRFY"
     gene            complement(1840..2043)
                     /locus_tag="VGA15_27565"
     CDS             complement(1840..2043)
                     /locus_tag="VGA15_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591488.1"
                     /translation="MADKPVFVDLISEATRQTRRRFAPDVDKPEVKERSRLSHWPPDR
                     WEQWRIETLTPWKLRSWKFKNWD"
     gene            complement(2147..3553)
                     /gene="leuC"
                     /locus_tag="VGA15_27570"
     CDS             complement(2147..3553)
                     /gene="leuC"
                     /locus_tag="VGA15_27570"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX9591489.1"
                     /translation="MSQPTTLYDKIWNDHLVHEADDGTCLLYIDRHLVHEVTSPQAFE
                     GLRTTGRKVRAPQKTLAVVDHNIPTTDRSKPNPDPESIEQMRVMAENAKEFGIEYYNE
                     FDKRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IQKKAKNMRAVVDGQLPDGVTGKDIILAIIGEIGTAGGTGYVLEYAGEAIRALSMEGR
                     MTVCNMSIEGGARAGLIAPDEKAFEFLKGRPKSPKGEAWDAAMRYWETLRSDDGAHFD
                     HEIRLDAAKLPPIVTWGTSPEDVVSISGMVPDPDEIADEAKRLSKHRALNYMGLKAGT
                     KITDIKLDRVFIGSCTNGRIEDLRAAAKVVAGKTVNGNLNAMIVPGSGLVKEQAEAEG
                     LDKIFIKAGFEWREPGCSMCLAMNPDKLLPEERCASTSNRNFEGRQGFKGRTHLVSPA
                     MAAAAAIAGHFVDIREWR"
     gene            complement(3632..3886)
                     /locus_tag="VGA15_27575"
     CDS             complement(3632..3886)
                     /locus_tag="VGA15_27575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591490.1"
                     /translation="MDLTKSAGSPRAFLLRHTIQAISAGHRLIVATAGIVLENNMVAD
                     QTSIASGIASRIVIDDRSRLPGRFFGRFTTSATSELPRAL"
     gene            complement(3907..4308)
                     /gene="rplS"
                     /locus_tag="VGA15_27580"
     CDS             complement(3907..4308)
                     /gene="rplS"
                     /locus_tag="VGA15_27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX9591491.1"
                     /translation="MNLIQELEKEQFDRLSAAGKPIPEFGPGDTVIVNVKVVEGDRSR
                     VQAYEGVCIGRSGGGLNESFTVRKISYGEGVERVFPVMSPMIDSITVVRRGKVRRAKL
                     YYLRNLRGKSARIVEKKTDRAQAATAAAVGE"
     gene            complement(4425..5159)
                     /gene="trmD"
                     /locus_tag="VGA15_27585"
     CDS             complement(4425..5159)
                     /gene="trmD"
                     /locus_tag="VGA15_27585"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX9591492.1"
                     /translation="MTSIAPIWRATVLTLFPEMFPGPLGVSLAGKALASGLWSLEARD
                     IRDSATDRHRSVDDTPAGGGPGMVLRADVLAAAIDAADISPDRPRLLTSPRGRPLTQS
                     RVAELAAGPGPLIVCGRFEGVDQRVIEARNLEEVSVGDYVLSGGEIASMALIDACVRL
                     LPGVMGKLASGADESFSEGLLEYPQYTRPQEFEGHTIPEILLSGDHARVAAWRQAEAE
                     ALTRARRPDLWSVKTSQKRPKNTTDG"
     gene            complement(5291..5980)
                     /locus_tag="VGA15_27590"
     CDS             complement(5291..5980)
                     /locus_tag="VGA15_27590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591493.1"
                     /translation="MLRYVVLALIGLVASSALAQQAPPQAGTPGAPPVEPAKAVVPME
                     EPLPGDHWTYEVRDEITGKISATRANVVTEVTPTEISIRFEVVGTPNQGLNVYDRSWN
                     LINSGPWRYSPSDGTGIQKPLATGKTWTFQANDVNAGGGYIWNRSGRSKVVGQENLTT
                     RAGTFETFKIETTYSRRNVKDPTKKEEVTGQTWYAPAIDHWVKRAVITRVDNHLLVNN
                     TLELTEYGRKQ"
     gene            complement(6043..6738)
                     /locus_tag="VGA15_27595"
     CDS             complement(6043..6738)
                     /locus_tag="VGA15_27595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591494.1"
                     /translation="MLRCAFLAAIGLLATASSLPAQTAPNESPPAASPPAVEAPGSPE
                     RMEDAQPGDHWTYELRDDITGDVKSTITNTVTDVSASEVSTRIAQLGNSNAGYQTFDL
                     SWNLMTNGIWRYTPNDGSGIRPPLAVGKTWSFKATDLNSTAGLSWKRSGTAKVVAQES
                     VTTRAGTFDTFKIETSLQVQNANDPTKKVQAVQQAWYAPVINHWVKRTFVSRSDGRVR
                     DKNTVELVEYGRR"
     gene            complement(6927..>7071)
                     /locus_tag="VGA15_27600"
     CDS             complement(6927..>7071)
                     /locus_tag="VGA15_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HEX9591495.1"
                     /translation="IIEIAPPQGATMLLPFTNAVVPLVDIANGRVVIELPAEIDGGDE
                     GSI"
BASE COUNT         1222 a         2168 c         2192 g         1489 t
ORIGIN      
        1 gtcagtcact ctagctttac gatctgcgtt gcggtgaggc ggtcgaccag atccgccgca
       61 cgtcgctggc gtgttgtcca atgaccaacg gaggaaacat atgaacgtga tgagagttct
      121 ggggcttgcg gccgtcgccg cattgctcat gctgaccgct cccgccgaac gcgcccaggc
      181 cctatcgctg gccagtcccg gcgccgctgc cgcggttcag gatgacacca gggagatgac
      241 caccgaagtg cgctggcacc atcactggca ccaccgccat tggggctatc atcggcactg
      301 gcatcaccgg cattggcatc atcgccactg gcatcgtcac cactggcatc atcgccattg
      361 gcatcatcgc tactggtgat atcaggcgcg acgctagggc cttttccgtt tcgatagaat
      421 cgaatcgggg ctctagattc ttgatttgac gcgttttctt gacgcgaacc ggtttccacc
      481 cccggatcaa gtccgagggc atgcttcgct tgaaaacgct ctggtgtgcg tgagtgagat
      541 tgagacaggt ccgcggtccg ggcctgtcgt tacgtggtga tcgatccagc aaaaggaaac
      601 gttgccttga agcgatggat tggagcggcc gtcatggcag cggggctggc gctctcaggg
      661 ccggttgcaa ccgggagcgt cgccgccaat tcgaaagttg gcttgcacaa agttggcttg
      721 ctcgcagtag gcctgcaccc aactgacaca tcgaaggcaa ccgacctcag cgcgcggcgt
      781 cgcgtccggc attaccggca tgatgcctac catccctatt accgcgctta ttactacgac
      841 cgaccttatg actatcggcc ttattcctac gctgcgccgg tgccgttctt cctcggcatt
      901 gggtttgggc cgcggtggta accgccgact cttgaatatt tgcgatctta ggcggcgcga
      961 tcgatcatct tgagctgtac gatcacgtag acggtcatgg cctctcctca tcgggtagcg
     1021 cgggctgatg caaccgaacc gattcgttca tcattgcggc ccgtgcattc acggcgcgtt
     1081 cacgtcgtta tcgctaattt aacgttggga cgaagcgcaa atgaacgcgt cgaaggcggg
     1141 ccgctgcatg gccttggctt ttgtcgccca atccggggag gttcacgcat gacgaggatt
     1201 ccagatccaa gacgcggcgt ccgttgcctt gtccgttctt tgggctttgc ggctgccacg
     1261 acgctgatgc tgtcgttcgc aaccgaccag cgcgccgaag cgctatcgct gatcagtccg
     1321 ggcgcggcgc cagcagtgac acacacctcc gacgggctga cgaccgaagt ccgcggcggc
     1381 ggccatggcg gtggtggtca tggtggcggt ggtcatggcg gtgggggagg ccacggtggc
     1441 ggcggttttc acggtggcgg cggcttccat ggtggaggtg cggcgtttca cggcggcgga
     1501 tttcgctccg ctcatgtttt ccacggcggc ggcttccgcc acggtgggtt cgggtttcgg
     1561 caccatcgct ttcaccggca cttctacgcg tcctattacg attacccgta ttattattcg
     1621 taccatcgct gccgggtgat ctggacctat tatggcccgc gcaggatctg ccactatcgc
     1681 cactggcatc atcgccacta ctggcatcac cactactggc gtcaccatca ccggcatcat
     1741 cgcttctatt gatcagacag gaagtgcggt gggtcgaacc aatcaggcgc cctcatgggc
     1801 gcctgatttg tgtctgccgc gaacaaactt tgccgcgctt caatcccagt tcttgaattt
     1861 ccaggatctg agcttccaag gtgtcagcgt ctcgatgcgc cactgctccc agcggtcggg
     1921 cggccagtgg ctgagacgcg acctttcctt gacctcgggc ttatcgacgt caggcgcgaa
     1981 tcggcgccgc gtctggcgcg tcgcctcgct gatcagatcg acgaaaaccg gcttgtcggc
     2041 catggtccgc tcctgcgaaa cggctcgcct ttcgcaagga tcagtctgct cccaactgct
     2101 taaggactcc cttcgggaat gcctcgacct tgaagcaaga ggtgctttac cgccactccc
     2161 tgatatcgac aaaatgcccg gcgatcgccg ctgccgccgc catcgccggc gacaccagat
     2221 gggtgcggcc cttgaatccc tgacggccct cgaaattgcg gttcgaggtc gaggcgcagc
     2281 gctcttccgg caatagcttg tcggggttca tcgccagaca catcgagcag cccggctcgc
     2341 gccattcgaa gccggccttg atgaaaatct tgtcgaggcc ttcggcttcg gcctgctcct
     2401 tcacgaggcc cgagcccggc acgatcatcg cattgagatt gccgttgacg gtcttgccgg
     2461 ccaccacctt cgccgctgcg cgcaaatcct cgatgcggcc attggtgcag gagccgatga
     2521 agacgcgatc gagcttgatg tcggtgatct tggtgccggc cttcaggccc atgtagttca
     2581 gcgcgcgatg ctttgagagc ctttttgctt catcggcgat ctcatcggga tccggcacca
     2641 tgcctgatat cgacaccacg tcttccggcg aggtgcccca ggtcacgatc ggcggcagct
     2701 ttgccgcgtc gagcctgatc tcgtgatcga aatgcgcgcc gtcgtcagag cgcagcgttt
     2761 cccaatagcg catcgcggcg tcccaggctt cgcccttcgg cgatttcggg cggcccttca
     2821 aaaattcaaa cgccttttcg tcgggcgcga tcaggccggc gcgggcgccg ccttcgatcg
     2881 acatgttgca gaccgtcatg cggccttcca tggacagcgc gcggatcgcc tcgccggcat
     2941 attccagcac atagccggtg ccgccggcgg tgccgatctc gccgatgatc gccagaatga
     3001 tgtccttgcc ggtgacgccg tcgggcaatt gcccgtcgac gacggcgcgc atgtttttcg
     3061 ccttcttctg gatcagggtt tgtgtcgcca gcacgtgctc gacttccgac gtgccgatgc
     3121 cgtgcgccag cgcgccgaat gcgccatggg tggaggtgtg gctgtcgccg cagacaatgg
     3181 tggtgccggg cagcgtaaag ccttgctcgg ggccgatgac gtgcacgatg ccctgacgct
     3241 tgtcgaactc gttgtaatat tcgatgccga actctttggc gttctcggcc atcacccgca
     3301 tctgctcgat gctttccgga tccggattgg gctttgagcg gtcggtggtg ggtatgttgt
     3361 gatcgaccac ggccagcgtc ttctgcggcg cgcgaacctt gcggccggtg gtgcgcagac
     3421 cctcgaaggc ctgcggcgag gtcacctcgt gcaccagatg acggtcgata tagagcaggc
     3481 aggtgccgtc gtcggcttcg tgaaccagat gatcgttcca gatcttgtcg tacagcgtgg
     3541 tcggttggga catgagcgta agctccaagg aatattttgc gtgaacacgg aaagcgcgga
     3601 tacgcgcaat tgaaaacaag ccggcgcggc gtcagagtgc gcgcggaagt tccgatgttg
     3661 ccgaggtcgt gaaccgtccg aagaagcggc cgggcaggcg gctgcgatcg tcgatcacga
     3721 ttcgactggc gatccctgag gcgatgctgg tctgatctgc aaccatgtta ttttctaaca
     3781 caatgccggc cgtcgcgaca atcaatcgat ggccggccga tatggcttgg atggtgtggc
     3841 gcaacaaaaa agcgcggggt gaacccgcgc ttttcgtcaa atccattgcg gacttaagac
     3901 taagaattac tcgccgacgg cggcagcagt ggcggcctgc gcgcggtccg tcttcttctc
     3961 gacaatgcgg gccgacttgc cgcgcagatt gcgcaggtaa tacaatttgg cgcggcgcac
     4021 cttgccgcgg cgcaccacgg tgatcgagtc gatcatcggc gacatcacgg gaaatacgcg
     4081 ttccacgcct tcgccgtagg aaatcttgcg caccgtaaag ctctcattga gcccgccgcc
     4141 ggagcggccg atgcagacgc cctcataggc ctgcacgcgc gagcggtcgc cttcgaccac
     4201 cttgacgttg acgatcacgg tgtcgcccgg cccgaattcc ggaatcggct tgccggctgc
     4261 ggacagtcgg tcgaactgct ctttttcgag ctcttgaatg aggttcatcg caaaatctcc
     4321 atcgggcggc gcgccccagc ctgcgaggcg cgggctgcgc gtacgtccat ttatcctgcc
     4381 attgcgcgga tttggcgctc atatacggca aaggccgcgg cttgtcaccc atccgtcgtg
     4441 ttttttgggc gtttttggct ggtttttact gaccagagat cgggccgccg cgcccgggtc
     4501 agcgcctcgg cctcggcctg gcgccaggcc gcgacccggg cgtggtcgcc ggacaacagg
     4561 atttccggga tggtgtggcc ctcgaactcc tgcgggcggg tatattgcgg gtattccaat
     4621 aggccctccg aaaagctttc gtccgcgccg gaggcgagct tgcccatcac gcccggcagc
     4681 agccggacgc aggcatcgat cagcgccatg ctcgcgatct caccccccga aagcacgtag
     4741 tcgccaaccg aaacttcctc gaggttacgg gcctcgatca cccgctggtc gacgccctcg
     4801 aaccgcccgc agacgatcag tgggcccggc ccggccgcaa gttccgcgac ccgggactgg
     4861 gtcaatggcc gaccccgcgg actcgtcagc aggcgaggtc gatctgggga gatatccgcc
     4921 gcgtcgatcg cggccgccag cacgtcggcg cgcagcacca tgccggggcc gccgccggcc
     4981 ggggtatcgt cgacgctgcg atgccggtca gtggcggaat ccctgatgtc acgcgcctcc
     5041 agcgaccaga gcccggacgc cagcgccttg ccggcgaggc tgacgccgag cggacccggg
     5101 aacatctcgg ggaacagcgt cagcacggtc gcgcgccaga tgggagctat ggaagtcatg
     5161 ccttccaatg gcgtgtccgg ccggagccgt caaaccggtt gcggcgcttc ctcggaacat
     5221 cggcatcggc gcgcgggtga gactaacggc accaacctcg gaatattgat ccggcggcgg
     5281 cgtccgggtc tcattgctta cgaccatatt cggtaagttc gagggtattg ttgacgagca
     5341 gatggttgtc gacccgagtg ataacggcgc gcttgaccca atgatcgatc gcaggcgcgt
     5401 accaagtctg tccggtcact tcttccttct tggtcgggtc tttgacgttg cgcctcgaat
     5461 aggtagtttc gatcttgaac gtctcgaacg tgccggccct ggtggtcagg ttttcctggc
     5521 cgacgacttt tgaacggccc gatcggttcc agatgtatcc acccccggca ttgacgtcgt
     5581 tggcctggaa agtccacgtc ttgccggtcg cgagcggctt ttggattccg gtgccgtcgc
     5641 tcggcgagta tcgccaggga ccggagttta tcaaattcca ggatcggtca taaacgttga
     5701 ggccttggtt gggggtgccc acgacctcga agcggatgct gatttcggtc ggcgtcacct
     5761 ccgtgacgac attggcgcgg gtggcgctga ttttcccggt aatttcgtct cgtacctcgt
     5821 aggtccaatg gtcgccgggc aacggctcct ccatcggaac aactgctttt gcaggctcga
     5881 ccggcggggc cccgggcgtt ccggcttgcg gcggggcctg ttgcgccaac gcgctactgg
     5941 cgacgaggcc gatcagcgcc agaacgacgt aacgaagcat tggcgtctcc cacatggccg
     6001 cttgttattc ctgcgagggc cgcagtgcgt gtgccggcgc aactagcggc ggccatactc
     6061 gaccagctct acggtgttct tatcccgaac gcgtccgtct gaccgcgata cgaaagtccg
     6121 cttcacccaa tgattgatca ccggtgcgta ccaggcctgc tgcacggcct gtactttctt
     6181 ggtcgggtca ttcgcgtttt gtacttgaag ggaggtctcg attttgaagg tgtcgaacgt
     6241 gcccgctctg gtcgtgacgc tttcctgcgc gaccaccttc gcggtgcccg agcgcttcca
     6301 gctaagtccg gccgtgctgt tgagatcggt ggctttgaac gaccaggttt tgcccaccgc
     6361 cagcggtggt cgaatgccgc tgccgtcatt gggcgtatac cgccagatgc cgttggtcat
     6421 cagattccag gaaagatcga aggtctgata gccggcgttg gaattgccga gctgcgcaat
     6481 ccgggtgctg acctcggaag ccgagacgtc ggtcacggta ttggtgatgg tcgatttgac
     6541 gtcgccagta atgtcgtccc gaagctcgta ggtccaatgg tcgcccggct gcgcatcttc
     6601 catgcgctcg gggctaccag gcgcttcgac agcaggcggg gacgccgcgg gtggggactc
     6661 gttgggcgcc gtttgggccg gcagggaact cgccgtggcg agcaggccga tggccgcgag
     6721 gaaagcgcaa cgcaacatgg aacatctctt gtgcagtggc agcagcaaat cgcgaaagaa
     6781 ttgccgcagg atgaggcacc aaaaatgccc gagccgaaaa ttgaacgcaa gtattttgta
     6841 attttcagcc gcagtctgtc ttaattttaa tgtcataatt tgagtattgg gccgatggcc
     6901 aaagcgaagc cagcgatgag ccggaactaa atcgatcctt cgtctccgcc gtcgatctcc
     6961 gccggcaatt cgatcaccac acgtccgttc gcgatatcga ctagcggcac cacggcattg
     7021 gtgaacggca gcagcatggt cgcgccctgc gggggcgcga tctcgatgat g
//
LOCUS       DASWGM010001023         8543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1093675,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001023 DASWGM010000000
VERSION     DASWGM010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093675"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VGA15_27605"
     CDS             complement(<1..205)
                     /locus_tag="VGA15_27605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX9591496.1"
                     /translation="MTAAEALINAPEFTVSELSSALKRTVEDAYGHVRVRGEISGFRG
                     AHSSGHCYFALKDDGAKIEAVIWK"
     gene            350..1354
                     /locus_tag="VGA15_27610"
     CDS             350..1354
                     /locus_tag="VGA15_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9591497.1"
                     /translation="MPRSLIRGSLLAVGLLLSVVGAAALLLTALVATPLKTLPELRSV
                     SASRKSVDFSTLPSVERFQARDGTMLGFRHYAASGPATGRGAIVIHGSSGSSGTTIHA
                     LSAASAGRGVETWAIDIRGHGASGTRGDIGYVGQLEDDLADFVAVVRKTNPDAPLTLI
                     GHSAGGGFAPRVAASPIQNLFARTVLLAPYLGYDAPTNRPNSGGWASADVPRILGLLA
                     LRKLGIDCCEALPTLAFAVPPNSENILVPVYSDRLMRNFATRGYQADLAAASKPLTIV
                     SGADDELMLADKYAEAVRSIAPQVNVKLIDGVNHMGIVSSPAAISVIADDVATTGLNS
                     "
     gene            1351..1935
                     /locus_tag="VGA15_27615"
     CDS             1351..1935
                     /locus_tag="VGA15_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591498.1"
                     /translation="MTSSIDPKEAAKEAAKEAASALSDIDSIVHRVRQSQIYQLASLM
                     LILWGALVFAGNIASFLWPRAAGYVWIAVNATGVVGSFAVGAFTSRRLSLPSFDFRMV
                     AAFLLFFAFGILWSIVLGHFTPRQLGVFWPTYFMMIYTIVGLWVGAAFVAIGLGISVL
                     TMIGYFFSGDVFDLWMAVVNGGGLILGGVWMRRS"
     gene            1939..2244
                     /locus_tag="VGA15_27620"
     CDS             1939..2244
                     /locus_tag="VGA15_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX9591499.1"
                     /translation="MAELDDIIHQPLRLQIMAALNALPGTTGLEFAKLKKLTGATDGN
                     LGAHIETLAKAGYVAVEKAFVGKKPQTTVTATAVGRGAFARHVATLQEIIAASAREN"
     gene            2493..3773
                     /gene="purD"
                     /locus_tag="VGA15_27625"
     CDS             2493..3773
                     /gene="purD"
                     /locus_tag="VGA15_27625"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX9591500.1"
                     /translation="MHILLLGSGGREHALAWKIAASPLVTKLWCAPGNAGIAREAECV
                     ALDIGDHAAVIDFCRTNAVDLVVVGPETPLAAGIVDVLTAAGIKAFGPSRQASQLEGS
                     KGFTKALCTEFNIPTGAYGRFTNAADARAYVRAQGAPIVVKADGLAAGKGVVVAMTMA
                     EAEAAIAMMFDGAFGAAGAEVVIEEFLAGREISFFALSDGETAIALASAQDHKRVFDH
                     DEGPNTGGMGAYSPTPFVTDEIHAEIMAKIIVPTVNGMKARGTPFRGVLYAGVMLTPD
                     GPKLFEYNVRFGDPECQVLMLRMMSDIVPAFLASCDRQLKNFDLRWFPDAALTVVMAA
                     KGYPGDYAKGTRIDALDDAAKVEGVEIFHAGTVSKDGHVLANGGRVLNVCASGKSIAE
                     AQRRAYEAVDRIKWPEGFCRRDIGWQAVEREKTG"
     gene            complement(3937..5169)
                     /locus_tag="VGA15_27630"
     CDS             complement(3937..5169)
                     /locus_tag="VGA15_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9591501.1"
                     /translation="MSIDFEVPAEAKAIREKVRKWVQEECIPAEKELDTKPLDEVLGK
                     LRKKARAQGLWCPFIPREYGGMGLGPLANALVQMELGESSLGALSMNTQGPDDASMMT
                     ILTHGTEYQKEKFLKPLLNGDKRICFSMTEKAAGADATGMQTRAVKDGNANYILNGEK
                     WFSSSASVADMALVMAKTDPDAPRHKRFSTFIVELPNPGYNIKRNIKNMAIEGPHADV
                     IHGGHSEIEIRDLKVPAENLLGGEGNGFNMGQHRLAYGRLRHGMHNIAKAQRALDMAV
                     AHVTKRSTFGVLLADRQAVQFMLAECASQLYIGRLMLLHIAYKAEKGMDLTQENSIAK
                     VYLAHMVHHVIDTAIQLHGALGYSQDTILAKWYTQVRSQRLVDGPDEVHRWKVGRNVI
                     KAFREHGTTASAAGGDLI"
     gene            5168..5410
                     /locus_tag="VGA15_27635"
     CDS             5168..5410
                     /locus_tag="VGA15_27635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591502.1"
                     /translation="MTFPRSGLLMVCLAGWLISGISQRQRQTKFKATVVIPAQAGIQY
                     AATPVVGCDVGVYCIIRWSLSSGAHSRDPLADDVRV"
     gene            5413..6318
                     /locus_tag="VGA15_27640"
     CDS             5413..6318
                     /locus_tag="VGA15_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9591503.1"
                     /translation="MPDLADLFPGYASEWINTSSGRIFARVGGKGPPLLLLHGFSETH
                     VMWHRVAPRLADRFTLIIADLPGYGWSDMPESDKDHTPYTKRAMARTIVEAMEQLGHV
                     HFALAGHDRGGRVAYRLALDHPGRLSRLAVLDILPTYNYWERMNRLYALKIYHWTFLA
                     QPFPLPETLIGGNPDFFLRQKMASQTKSKTLDAIDPRALEHYLAAFRDPSRVHAMCED
                     YRAGAYADFEIDKKDRDAGNKITIPMLVLWGDAGIASAAATPLDTWKTWAANVSGAPV
                     ASGHFLTEENPDATAKALREFFAGS"
     gene            complement(6405..6851)
                     /locus_tag="VGA15_27645"
     CDS             complement(6405..6851)
                     /locus_tag="VGA15_27645"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX9591504.1"
                     /translation="MTAPSFMDLALKAAETARKSGEVPIGCVIVLNNEVIATAGNRTL
                     TDRDPTAHAELLAIRTATQVVGSERLVACDLYVTLEPCTMCAGAISLARIRRLYYGAA
                     DPKGGAVESGVRFFASPTCHHVPEVYSAVGESEAATLLRDFFKARR"
     gene            7020..>8543
                     /locus_tag="VGA15_27650"
     CDS             7020..>8543
                     /locus_tag="VGA15_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX9591505.1"
                     /translation="MPRDNDKDNASSRGRRDRPSGGKGRSGAARGPDKKFAKRDFAGK
                     GDGEKRPYAGKSDGAKSYGKKPYAGPGKPYAGKREGPPPRRDDGGAPRPRGDRPFADR
                     PPRFNRDDRPRGDRSEPRKDFRPRDDRGGEKRPFTPRGDRPNSGRDDRAPRSDARPAR
                     QFSDRKFADKKPYAPRDRGGEKRPSSPRGEGFRKDKDGDRPRADRPYSARPSRDGDRP
                     RGDRPERKFGGDKKFSRGAPDRKSDRGDRNSDRGDRGESKPWQKRDASSPDHAGRNSR
                     PPRDGARNFDRPKFEKPRFDKPREDRGGDERPRFSRPRQDRPQGDRPFRDRPKFERPR
                     ESGSSWHEHPRSEPRSSDRSSDRPRRDNEDDSKVFVKRPAFGGRGEYRERKPDERRPP
                     RPPREKKSGERIAKVVSRAGLASRRDAEEWIVQGRVTVNGRVINSPALDVTANDVITV
                     DGKPLPPRERTRLFMFHKPRGLMTTHADPEGRPTVFDNLPEGLPRLISIGRLDFNTEG
                     "
BASE COUNT         1539 a         2656 c         2693 g         1655 t
ORIGIN      
        1 ccttccagat caccgcctcg atcttggcac cgtcgtcctt cagcgcgaaa tagcagtgcc
       61 cggaggagtg ggcgccgcga aaaccggaga tttcgccgcg cacccggaca tgcccatagg
      121 cgtcctccac cgtccgcttc aaggcggagg acagttcgga caccgtgaat tccggtgcgt
      181 tgataagggc ttcggctgcg gtcatctcca atatggaatc ggccttttcg ggccgtggtg
      241 caagggtagg cgctttggca ttcaccgata ttcaacactt gaaaactcat ttactctgta
      301 atatagagtt ctctgcatac ctcatatttt accagatgaa ggaatgatca tgccgagatc
      361 gctgatacgt gggtctttgc tcgccgtggg gctgctcctg agcgttgtag gcgcggcggc
      421 gctgctgctc accgccctgg tcgccacgcc gctaaaaact ctcccggagc tgcgttccgt
      481 ctcggcatct cgcaaaagcg tcgatttctc gaccttgcct tccgtcgaac ggttccaggc
      541 ccgcgacggc accatgcttg gtttccgtca ttatgccgcg agcggcccgg ccacggggcg
      601 cggagccatt gtgattcatg gctcatccgg atccagcggg accacgattc acgcgctgtc
      661 ggccgcttcg gcggggcgcg gcgtcgaaac atgggcgatc gacattcgcg gccatggcgc
      721 ctcggggacc cgcggcgata tcggctatgt cggccaactc gaggacgatc tcgccgattt
      781 cgttgccgtc gttcgcaaga ccaaccctga cgcgccgctg accctgatcg gtcactccgc
      841 cggcggcggc ttcgcgccgc gggtcgcggc atcgccgatc cagaacctgt tcgcccgcac
      901 cgtgctgctt gcgccctatc tcggctatga cgctccgacc aaccggccga attccggcgg
      961 ctgggccagc gccgacgtgc cgcgcattct cggattgctg gcgctgcgca agttgggcat
     1021 cgattgctgc gaggcgctgc cgacgcttgc cttcgcggtg ccgccgaatt cggaaaacat
     1081 cctggtgccg gtctattccg atcggctgat gcgcaatttc gcaacgcgcg gctatcaggc
     1141 tgatctcgca gccgcgtcca aaccgctaac gatcgtctcg ggcgccgacg acgaattgat
     1201 gctggcggac aaatatgccg aggcggtgcg ctccatcgcg ccgcaggtca atgtcaagtt
     1261 gatcgacggc gtcaatcata tgggaatcgt gagcagcccc gccgccatat ctgttatcgc
     1321 cgacgatgtc gcgacaacag gcctgaattc atgacctcca gcattgatcc caaggaagct
     1381 gccaaggaag ctgcgaagga agctgcgtcg gcactctcgg atatcgactc catcgtccac
     1441 cgcgttcgtc agtcccaaat ctaccaactc gccagcctga tgctgatcct gtggggcgcg
     1501 ttggtgttcg cgggcaacat cgcgtcgttt ctctggcctc gcgcggccgg ttacgtctgg
     1561 atcgccgtca atgcgacggg ggtggtggga tcgttcgcgg ttggcgcctt caccagccgc
     1621 cgactgagcc tgcccagctt cgatttcaga atggtcgccg cattcctgct gttcttcgcc
     1681 ttcgggattc tctggtcgat cgtgctcggg catttcacgc cgcgccagct cggcgtgttc
     1741 tggccgactt atttcatgat gatctacacc atcgtgggat tgtgggtcgg tgccgccttc
     1801 gtcgcgatcg ggcttgggat cagcgtgctg acgatgatcg gctatttctt cagcggtgac
     1861 gtgttcgacc tgtggatggc cgtggtcaat ggcggcggac tgatcctggg cggcgtctgg
     1921 atgcgccgga gctgaccgat ggccgagctc gacgacatca tccatcagcc cttgcgcttg
     1981 caaatcatgg cggcgctcaa cgcgctgccc ggaacgaccg gactggaatt cgcgaagctg
     2041 aaaaagctca ccggcgccac cgacgggaat cttggcgccc atatcgaaac gctggcgaaa
     2101 gcaggctatg tcgcggtcga aaaggccttt gtcggcaaaa aaccgcagac cacggtgacc
     2161 gccaccgcgg tggggcgcgg cgccttcgcc cgccacgtcg cgacgctgca ggaaatcatc
     2221 gcggcctcgg ccagggaaaa ctaaaacacg ctcgtcattc cggggcgcga gccaacgggt
     2281 ccggcctttg gccggcccga tgacaggctc cgcgagcgag cccggaatcc ataaccacga
     2341 tcgggagtat ggattccggg cctgcgccaa aaggcgcatc ctcagatgtg caattgcaca
     2401 tcggggaatg acggtgtttg atttgtcccc cttgcggcaa gagggagttt cgtgcgaccc
     2461 gactcgggtc cacaaccatc attggtttcc tcatgcacat tctcttgctc ggttccggcg
     2521 gccgcgaaca cgctctggcg tggaaaatcg cagcgtcgcc tttagtgaca aaactctggt
     2581 gcgcgccggg caatgccggc atcgcgcggg aggccgaatg cgtggcgctg gatatcggcg
     2641 atcatgccgc ggtgatcgat ttctgccgga ccaacgccgt tgatttggtg gtggtcggcc
     2701 cggaaacgcc actcgccgcc ggcatcgtcg acgttctcac ggccgccggc atcaaggcgt
     2761 tcgggccgag ccgacaggct tcgcagctgg agggctccaa gggatttacc aaagcgctct
     2821 gcaccgagtt caatattccg accggcgcct atggccgctt caccaatgcg gccgacgcgc
     2881 gggcttacgt ccgcgcccaa ggcgcgccga tcgtggtcaa ggccgacgga ttggcggcgg
     2941 gcaagggcgt ggtggtggcg atgacgatgg cggaagcgga agccgccatc gccatgatgt
     3001 tcgatggcgc gttcggcgcg gccggcgccg aagtggtcat cgaggaattt ctggctggcc
     3061 gcgaaatcag ctttttcgcg ctatccgacg gcgagaccgc gatcgcgctg gcctcggcac
     3121 aggaccataa gcgggtgttc gatcacgacg aggggccgaa caccggcggc atgggcgcct
     3181 attcgccgac gccgttcgtg acggacgaaa tccacgccga gatcatggcg aagatcattg
     3241 ttccgacggt gaacggcatg aaggcgcgcg gcacgccgtt tcgcggcgtg ctgtatgccg
     3301 gcgtgatgct gacgccggac ggtccaaaat tgttcgaata caatgtccgc ttcggagatc
     3361 cggaatgtca ggtgctgatg ctgcggatga tgtcggacat cgtgccggcg tttctcgcct
     3421 catgcgacag gcagttgaag aatttcgacc tgcgctggtt tcctgacgcg gcgctgacgg
     3481 tggtgatggc ggcaaagggc tatcccggcg attacgccaa gggcacgcgc atcgacgccc
     3541 tcgatgacgc cgccaaggtc gagggtgtcg aaatctttca cgccggcacg gtatcgaagg
     3601 acggacatgt tctggccaat ggcggccgcg tgctcaacgt ctgcgcgtcg ggcaaatcca
     3661 tcgccgaggc gcagcgccgc gcttacgagg cggtcgaccg catcaaatgg ccggaaggtt
     3721 tttgccgccg cgacatcggc tggcaggcgg tggagcggga gaagacgggg tagttatcat
     3781 cccgaacggt gcggcatacc ccggtcgtca tacgcgggct tgacccgcgt atccatcaat
     3841 ttcgcaaaat acattttcga aaaggatgga ttgccgggtc aagcccggca atgacaccgg
     3901 ttttcatctt cacgcgcgcg aagacgcgac cttcgttcaa atcaaatccc cacccgcggc
     3961 ggacgccgtg gtgccgtgtt cgcgaaaagc cttgatgacg ttgcggccga ccttccatct
     4021 gtgaacttcg tcgggaccgt cgaccaggcg ctgcgagcgg acctgggtgt accatttcgc
     4081 cagaatggta tcctggctgt agccgagcgc gccgtgcaac tggatcgcgg tgtcgatgac
     4141 gtggtggacc atgtgcgcca gatatacttt cgcgatggaa ttctcctggg tcaggtccat
     4201 gcccttttcc gccttgtagg cgatgtgcag cagcatcagc cgcccgatgt agagctggct
     4261 ggcgcattcg gcgagcatga actgcaccgc ctgccggtcg gcgagcagca cgccgaaggt
     4321 cgaacgcttt gtgacatgcg cgaccgccat gtcgagcgcg cgctgggcct ttgcgatgtt
     4381 gtgcatgcca tggcgcagcc ggccgtaggc gaggcggtgc tggcccatat taaaaccgtt
     4441 gccttcgccg ccgagcagat tttcggccgg aaccttgaga tccctgattt cgatttcgga
     4501 atggccgccg tggatcacgt cggcgtgcgg gccttcaatc gccatgttct tgatgttgcg
     4561 cttgatgttg tagccggggt tcggcaattc gacgatgaag gtcgaaaacc gcttgtggcg
     4621 cggcgcgtcc ggatcggtct tggccatcac cagcgccatg tcggcgacgc tggccgatga
     4681 ggaaaaccat ttttcgccgt tgaggatgta gttggcgttg ccgtccttca ccgcgcgggt
     4741 ctgcatgccc gtggcatcgg cacccgccgc cttttccgtc atcgagaagc agatgcgctt
     4801 gtcgccgttg agcaggggtt ttaagaattt ttccttctga tactccgtgc cgtgggtgag
     4861 gatggtcatc atcgaggcgt cgtcgggtcc ctgggtgttc attgagagcg cgccgagcga
     4921 actttcgcca agctccatct gcaccagcgc gttggcgagc gggcctaagc ccatgccgcc
     4981 atattccctg gggatgaacg gacaccacag accttgcgcg cgggccttct tccgcagctt
     5041 gcccaagact tcgtcgagcg gcttggtgtc cagttccttc tccgcgggga tgcattcttc
     5101 ctgcacccat ttgcggactt tttctcggat cgccttggct tcggccggga cttcgaaatc
     5161 gatcgacatg acatttcctc gttctggttt attgatggtt tgtcttgcgg gatggcttat
     5221 atccgggatc agccagcggc aacgacaaac aaagtttaaa gcaacggtcg tcatacccgc
     5281 gcaagccggc atccagtatg cggcgacgcc tgttgtcggt tgcgatgttg gtgtttactg
     5341 catcatccgc tggagcctgt catcgggcgc gcattcgcgc gacccgttgg cggatgacgt
     5401 gagagtgtga aaatgcctga tctcgccgac ctgtttccgg gctacgcgtc cgaatggatc
     5461 aacaccagct ccgggcgtat ttttgcgcgc gtcggcggca agggaccgcc gctattgctg
     5521 ttgcacggct tttccgagac gcatgtgatg tggcaccgcg tggcgccgcg gctcgccgac
     5581 aggttcacgc tgatcatcgc cgatctgccg ggttatggct ggtcggacat gccggagagc
     5641 gacaaggatc atacgcccta caccaagcgt gcgatggcgc ggacgatcgt cgaggcgatg
     5701 gagcaactcg gccatgtgca ttttgctctc gccgggcacg atcgcggcgg ccgcgtcgcc
     5761 tatcggctgg cgctcgatca tcccggccgg ctgtcgcggc ttgcggtgct cgatatcctg
     5821 ccgacctata actactggga gcgcatgaac aggctttacg cattaaagat ctatcactgg
     5881 acgtttctgg cgcagccttt tccgttgccg gaaaccctga tcggcggcaa tcctgatttc
     5941 ttcctgcgcc agaaaatggc aagccagacc aagtcgaaaa cgctcgatgc gatcgatccg
     6001 cgcgcgctgg agcattatct tgcggcattc cgcgatccct caagggtgca cgcgatgtgc
     6061 gaggattatc gcgccggcgc ctatgccgat ttcgagatcg acaagaaaga tcgcgacgcc
     6121 ggcaacaaga taacgatccc gatgctggtg ctgtggggcg atgccggcat cgccagtgcc
     6181 gccgcgacgc cgctcgacac ctggaagaca tgggcggcca atgtgagcgg agcgccggtt
     6241 gcttccggtc atttcctgac cgaggaaaat ccggatgcga ccgcgaaggc gttgagggag
     6301 ttttttgcgg gctcgtgact gcgtagcccg gatggagcgc agcgtaatcc gggacggctc
     6361 tacgttgttc ccggattacg ctgcgctcca tccgggctac ggcgctatct tcgcgccttg
     6421 aaaaaatctc gcagcagcgt cgcggcttcg ctctcgccga cggccgaata aacctcgggc
     6481 acatgatggc aggtcggtga cgcaaaaaac cgcacgccgg attccacggc gccgcccttg
     6541 ggatcggccg cgccgtaata cagccgccgg atacgcgcca acgagatcgc gccggcgcac
     6601 atcgtgcacg gctccagcgt gacatagagg tcgcacgcga ccagccgctc gctgccgacg
     6661 acctgggtgg ctgttcggat cgccagaagc tcggcatgcg cggtcggatc gcggtcggtc
     6721 aaagtgcggt tgccggccgt ggcgatcact tcgttgttga gcacgatcac gcatccgatc
     6781 ggcacttcgc cggattttcg tgccgtttcg gctgctttca gcgccaaatc catgaaagag
     6841 ggagctgtca tggctcgtat ccagccgaaa tgtctgctaa tagaggtgca ttcagcaaaa
     6901 gtggataccg gttttgcgtg agaatgcgct tcgagcgaag agcgcgcgcg gtatcgttgt
     6961 gccagcccgt ccacatcagc ctgattgcca attcacctat acccgaagcg agaccattca
     7021 tgccccgcga taacgataaa gacaacgctt cctcgcgcgg ccggcgtgat cggccctctg
     7081 gcggtaaagg ccgctcgggc gctgcccgcg ggcccgacaa gaaattcgcc aagcgcgatt
     7141 tcgccggaaa aggcgatggc gagaagcggc cctacgccgg caaatccgac ggcgccaaat
     7201 cctatggcaa gaagccttat gcggggccgg gcaaacccta cgccggcaaa cgcgagggtc
     7261 cgccgccgcg ccgcgatgat ggcggtgcac cgcgccctcg cggcgatcgg ccttttgccg
     7321 atcgtccgcc gcggttcaac cgggatgatc gtccgcgcgg cgatcgttcg gaaccgcgca
     7381 aggattttcg cccgcgcgac gatcgtggcg gcgaaaagcg ccccttcacc ccgcgcggcg
     7441 atcgcccgaa ttccggtcgc gacgaccgcg ccccgcgctc cgacgcgcgt cccgcccggc
     7501 aattctccga caggaaattt gctgacaaga agccttatgc gccacgcgac cgtggcggcg
     7561 agaagcgccc gagctcaccg cgcggcgaag gttttcggaa agataaagat ggtgaccggc
     7621 cgcgagcaga tcgcccgtac agtgcgcggc cgtcgcgcga cggcgatcgt ccgcgcggcg
     7681 atcgtcccga gagaaaattt ggcggcgaca agaaattttc ccgcggtgcg ccggatcgca
     7741 agagcgaccg aggggatcgc aacagcgacc ggggggatcg cggcgagtca aagccttggc
     7801 agaaacgtga tgcaagttcg cccgatcacg ccggacgcaa ctcccgtccg ccccgcgatg
     7861 gcgcacgtaa ttttgacaga ccgaaatttg aaaaaccccg tttcgacaaa ccgcgcgagg
     7921 accgcggcgg cgacgagcgt ccgcgctttt cgcgtccgcg ccaggatcgc ccccagggcg
     7981 atcgcccgtt tcgagaccgt ccgaaattcg agcgaccgcg cgagagcggc agttcctggc
     8041 acgaacatcc gcgcagcgaa ccgcgatctt ccgatagatc ttccgatcgg ccccgccgcg
     8101 acaacgagga tgacagcaag gtctttgtaa aacgtccggc attcggcggc cgcggcgaat
     8161 accgcgagcg caagcccgac gagcgccgtc cgccgcggcc gccgcgcgaa aagaaatccg
     8221 gtgagcgcat cgccaaggtg gtgtcgcgcg ctggtcttgc ctcgcgccgc gacgccgagg
     8281 aatggatcgt gcagggccgc gtcaccgtca acggccgcgt catcaattcg ccggcgctcg
     8341 acgtcaccgc caatgatgtc atcaccgtcg acggcaagcc gttgccgccg cgcgagcgca
     8401 cgcggctgtt catgtttcac aagccgcgcg ggttgatgac cacccatgcc gatcccgaag
     8461 ggcggccgac ggtgttcgac aatctgccgg aaggactgcc gcggctgatc agcattggcc
     8521 ggctcgattt caacaccgag ggc
//
LOCUS       DASWGM010001024         7549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_538421,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001024 DASWGM010000000
VERSION     DASWGM010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538421"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VGA15_27655"
     CDS             <1..316
                     /locus_tag="VGA15_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX9591506.1"
                     /translation="PDYALAKRLLPRGAGSIVSFGIKGGRPAGKKFIESLRLISHLAN
                     VGDAKTLVIHPASTTHQQMDAAQLKASGIGEELVRLSIGIESASDITDDLGQALRLSQ
                     KA"
     gene            320..1114
                     /locus_tag="VGA15_27660"
     CDS             320..1114
                     /locus_tag="VGA15_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9591507.1"
                     /translation="MQLSVNGVETFVATGGRDFESSLPTVVLLHGAGFDHTTWALHSR
                     WFAHHGYGVLAPDLPGHGRSSGAPLQAIADMADWTAAMLDAAGVAKAWLVGHSMGSLV
                     ALETAARHPAKVAALGLIGTAATMTVGPDLLKAAEANDHSAVDMVSIWGLGFQAGLGG
                     SLAPGLWMHSGAQRVLEQCRPDVLFNDLNACNAYRNALAAAAQIKVPATFILGERDMM
                     TPAKAGKTLAAALPNSRTVVISGAGHMMMVERPDELLAALQGYRAA"
     gene            complement(1099..1602)
                     /locus_tag="VGA15_27665"
     CDS             complement(1099..1602)
                     /locus_tag="VGA15_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9591508.1"
                     /translation="MKIDSFDTTILSELTVNARASHVELAERVGLSSTAIARRQKVLE
                     EEGFIRGYQAVLDLTRFGLSTTVLVRVTLESQNEDALKSFEAAVIGCPSVVRCFLMSG
                     SDDYIVIVLARDIEDFERIHRTELSRLPRVARIQSSFAMREVVNRAVPPVIFGGSRRQ
                     RSAQAAR"
     gene            1737..2852
                     /gene="ald"
                     /locus_tag="VGA15_27670"
     CDS             1737..2852
                     /gene="ald"
                     /locus_tag="VGA15_27670"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX9591509.1"
                     /translation="MRVGVPKEIKVHEYRVGLTPGTAREYVAAGHSVVVETNAGAGIG
                     ATNEAYRKVGAVIADTAAEIFASADMIVKVKEPQAAEWSQLREGQILFTYLHLAPDPE
                     QARGLMASGCTAIAYETVTDAKGGLPLLAPMSEVAGRLSIEAAGYALKRSAGGRGLLL
                     GGVPGVVPARVVVIGGGVVGTHAARMAVGLGAEVSVLDRSLPRLRELDELFGGRVRTR
                     FSTIEAIEQEVFSADVVIGAVLVPGASAPKLVSREMLKSMRQGSVLVDVAIDQGGCFE
                     TSHATTHADPTYVVDGIIHYCVANMPGAVPLTSSQALNNATLPFGLAIAGHGFAAVTQ
                     DPHLRAGLNVHRGHITNKAVADSLGLSCAPIEGVIAA"
     gene            3341..4792
                     /locus_tag="VGA15_27675"
     CDS             3341..4792
                     /locus_tag="VGA15_27675"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX9591510.1"
                     /translation="MAIGGQPGDHPKNGRRMMAVVRSELRKDSLSFLEALGQSVANVS
                     PTLTPALVVAVVAGMAGTASWLVYVFATIALVIVGINVGKLAKRIPSAGSFFIYVSRS
                     LGPSYGMLSAWAMLAAYIFTAMALTVATSILVKTFFTSLGFTTPIPSTLLYFLVSAGI
                     WAFACRDIKISARIGLTLEVISVAIILFVCVYTFAHFGFKIDTKQTHLEGIDFKSAAQ
                     AVVFAIFSYVGFESAATLGRETRNPTVNIPRAIVATAIFAGVFFVLTTYLIVQGFDDD
                     AAKLGASATPLADITSSISVGLTALVYFGAAISGFACALASLNAYGRMLFSLGRYQFV
                     HTSMGLVHHNFKTPYVALTSGALINFIVCAAFAGQAETDTFGWYGTLASYGFIIVYFL
                     CSVSAPVFLAKNGGATAGDKFVGGLGAVLMVLSLVGSLYPVPASPYNLFPYFFLVYIL
                     LGVSWFFILKARVPQAILGIEHDLEIAASTPAE"
     gene            5158..6882
                     /locus_tag="VGA15_27680"
     CDS             5158..6882
                     /locus_tag="VGA15_27680"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX9591511.1"
                     /translation="MSQQPSTLNASGWTLAVGASSSASATSEGADVYVIHKGEAFDRK
                     VFDRPGRGIIAAVARGIGAGPHAREAAHIALHGLLEGYFGAAATLGPGRAAGLALGSA
                     NAWMFGQSRADPGHPMAASLTALIFVGRRVRIVHVGDGRVYRRRGSQLTPLTSDHVHP
                     AADGTGLLTRLVGGDSELQMDYLEDACEPADRYFILSKGSFAATPSGRLADVLTATRT
                     ADEAAGLIVASIPDKTGASAATAIVIDVLKAPDSSFDEMAAAFLRLPIRGAPRDGENW
                     DGFLLGRTLYRSRYTTLKLARDTLGDKDVVLKIPLPSMLHDEVFRAGFLREAWVGATV
                     HSPRIASYIEVAPERRSSLYLVLPYYHGETLEARLSRTDPIAYLEGVGLALKLCAAVQ
                     DLAALQIIHRDLKPENILLLPDGEIKLLDLGLAYLPGVDEPDDDRLGGTTRYMAPELF
                     RKQPADQRSEVFSLGVTVYRLFSGGPFPFGQRETIPLQRLRPDLPAWLGQCMMSAMEM
                     NREKRFKDANEFARALENGLSQGAARPLGTLPPQHRRIDPLRLWQGLTFLFAAGFFVL
                     LIMLLRLR"
     gene            complement(6932..7144)
                     /locus_tag="VGA15_27685"
     CDS             complement(6932..7144)
                     /locus_tag="VGA15_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591512.1"
                     /translation="MSRFVVRFMKDVLGENGRETEICQGSLEIDASNEDQATELAKTK
                     FCETQALRHWSLHADRIQVKEADFPS"
     gene            complement(7279..>7549)
                     /locus_tag="VGA15_27690"
     CDS             complement(7279..>7549)
                     /locus_tag="VGA15_27690"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEX9591513.1"
                     /translation="LALRSNDLAVLLTAARAGLGLAVLPCMMARGVPDLVSVPTRLPP
                     LTRELWLLFHRDIGRSPAVRAVIDLITAITTAARGAFLGEQAAGS"
BASE COUNT         1351 a         2387 c         2329 g         1482 t
ORIGIN      
        1 tccggattat gcgctggcca aacgactgct gccgcgcggc gcggggtcga tcgtcagctt
       61 cggaatcaag ggcggccggc ctgccggcaa gaaattcatc gagagcttgc ggctgatcag
      121 ccacctcgcc aatgtcggcg acgccaagac gctggtcatt catcccgcca gcaccaccca
      181 tcagcagatg gatgccgcac agctcaaagc ctccggaatt ggcgaagagc tggttcggct
      241 ctcgatcggc atcgaatccg cctccgacat caccgacgac ctcggccagg cgctccgcct
      301 atcgcagaag gcttgagcca tgcagttgtc cgttaatggc gttgaaacct tcgtggcgac
      361 cggtggccgg gacttcgaat cctcgctgcc aaccgtcgtg cttctgcacg gcgccggctt
      421 cgaccatacc acctgggcct tgcacagccg gtggtttgcg catcacggct acggcgtgct
      481 ggctccggat ttgccgggcc acggccgttc gtccggcgcg ccccttcagg cgatcgccga
      541 catggcggac tggactgcgg caatgctcga tgccgccggc gtggcaaagg cgtggctggt
      601 cggtcattcc atgggatcct tggttgcgct cgagaccgcg gcgcggcacc cggccaaagt
      661 tgcggcgctc ggcctgattg gcacggcggc gaccatgacc gtgggacccg atctgctcaa
      721 ggccgccgaa gccaacgatc actccgccgt cgacatggtt tcgatctggg gccttggatt
      781 tcaggccggg ctgggcggca gccttgcgcc gggattgtgg atgcattccg gcgcccagcg
      841 ggtccttgaa caatgccggc ccgatgtgct gttcaacgat ctcaatgcct gcaacgccta
      901 tcggaacgcg ctcgccgccg ccgcgcagat caaggtcccg gccacgttca ttctcggcga
      961 acgcgacatg atgacaccgg cgaaggccgg caagacgctt gcagcggcgc tgccgaattc
     1021 gcgcaccgtg gtgatatcag gcgcgggcca catgatgatg gtcgaacggc ccgacgaact
     1081 gctcgcggcc ctgcaaggct atcgcgccgc ctgagctgac cgctgtcgtc gcgatccacc
     1141 aaatatcacc ggcgggaccg cccggttcac aacctcgcgc atggcgaaac tcgattgaat
     1201 ccgcgcaacc cgcggcaacc tggagagctc ggttcggtgg atgcgttcaa aatcctcgat
     1261 atcgcgcgcc aggacgatga cgatgtagtc gtcgctgccc gacatcagga agcagcgaac
     1321 caccgatgga cagccgatca ccgcggcttc gaacgatttt aaagcgtcct cgttttggct
     1381 ttccagcgtg accctgacca gaacggtggt ggacagcccg aaccgagtca gatccaatac
     1441 cgcctgatag ccacggatga aaccttcttc ctccagcacc ttctgccgcc gcgcgatcgc
     1501 cgtgctcgaa agcccgacgc gctcggcgag ttcgacgtgg ctggcccggg cattgaccgt
     1561 gagttcggag aggatggtcg tatcgaagct atcgatcttc atgcgttccg attcctgcgc
     1621 gaagtgttcg gatcatgccg gcttccggcg agaattcaga ttaatgcatc aagatcgcgg
     1681 ggattcaaac cggtatctga cctagaaacc tggtttcaaa acagggagct tccatgatgc
     1741 gtgtcggagt gccgaaggaa atcaaggttc acgagtatcg cgtcgggctc acacccggca
     1801 cggcccgtga atatgtcgcc gcaggccaca gcgtcgtcgt cgagaccaac gcaggcgcgg
     1861 gcatcggagc gaccaatgag gcctatcgca aggtcggagc tgtcatcgcc gacaccgctg
     1921 ccgagatttt cgcgagcgcc gatatgatcg tgaaggtcaa agaaccccag gctgccgaat
     1981 ggtcccagct gcgagagggc cagatcctct tcacctatct gcatctggcg cctgacccgg
     2041 aacaggccag aggtctgatg gcctcgggct gtaccgcgat tgcctatgag acggtcacgg
     2101 acgcgaaggg agggctgccc ctgctcgctc cgatgagcga ggtggcggga cgactttcca
     2161 tcgaagcggc gggctatgcc ttgaagcgga gcgccggcgg ccgcggcctg ctgctcggcg
     2221 gcgtaccggg cgtggttcct gcccgcgtcg tggtgattgg aggcggggtc gtcggcacgc
     2281 acgccgcgcg gatggccgtt gggctcggtg cggaagtcag cgtgctcgac cgttcgctcc
     2341 cgagactgcg tgaactcgac gagctgttcg gggggcgggt ccgcacacgc ttctccacca
     2401 tcgaggcgat cgaacaggag gttttctcag ccgatgtcgt gatcggcgcc gttctcgtgc
     2461 ccggcgccag cgcaccgaag ctggttagcc gggagatgct gaagtcgatg cggcagggtt
     2521 cggtgctcgt cgatgtcgcg atcgatcagg gtggctgctt cgagacatcc cacgcgacaa
     2581 cccacgccga tccgacctat gtggtcgacg gaattatcca ttactgcgtg gcaaacatgc
     2641 ccggcgcggt tccactgacg tcgagccagg ccttgaacaa tgcgacgctg ccgttcgggc
     2701 tggcgatcgc gggccatggc tttgcagccg tgacgcagga tccccatctg cgggcgggcc
     2761 tgaatgttca ccgcggccat atcaccaaca aagcggtagc cgacagtctt ggcctgtcct
     2821 gcgcgcccat cgagggcgtg atcgccgcct gattccgcct gagccctcgc ctcgcgtccc
     2881 aaccgtttcg aagccgcgga acgcgcaacc gatctcggct gtgccgcgaa cgcggccgtc
     2941 caggccgatt gcgcctgttg cggcgatgct gtggccgctt gccgacttat tcgatcttgg
     3001 tttttccgaa gcttggcccg ccgcttttgc atcggccgtc agtgacacgc caaaaagttc
     3061 gccttcgccg aggccagcgc tgaagaactc gatcggtcgc gcacgacgtc gcgtggttcg
     3121 acagcagccg cacaaatctt agacagagtg gacaggcgat gggctccggc gacacgccct
     3181 gcggccaagc acaattcttt cctgttatga aaagcaagtg ggcgtccccg aaaagcccgg
     3241 taaaccagca gttctcgcgt gcggcacgca tcttgctaag aaactatttt gcatgtcagg
     3301 aaatcggctc ggtctcacaa cccagcgggc ggaaccatgg atggcgatcg gcgggcagcc
     3361 gggagaccat cccaaaaacg ggagacggat gatggcggtg gtacggtcgg aactgcggaa
     3421 ggatagtttg tcgtttttgg aagcgctcgg tcagtcggtt gcgaatgttt cgccgacgct
     3481 gacgccggca ttggtggtcg cggtcgtggc aggcatggcc ggcacggcat cgtggctggt
     3541 ctacgtcttc gcgaccatcg ctctggtgat cgtcggcatc aatgttggca agctggccaa
     3601 acgcatcccg tctgccggct cgttcttcat ttatgtctca cgcagtctgg gtccatccta
     3661 cggcatgctg tccgcgtggg ctatgctggc ggcctatatc ttcacggcga tggctctgac
     3721 ggttgcgacc tccatcctcg tcaagacgtt tttcacctcg cttggcttca ccacccctat
     3781 tcccagtacg ctgctgtact tcctggtttc ggccgggatc tgggccttcg cctgccgtga
     3841 catcaagatt tcggcgcgca tcggcctgac gcttgaggtg atatcggtcg ccattatcct
     3901 gttcgtgtgc gtctacacgt ttgcccattt cggcttcaag atcgatacca agcaaactca
     3961 tctcgagggc attgacttca agagcgccgc gcaggcggtg gtgttcgcca tcttctccta
     4021 tgtcggcttc gagagcgcgg caacgctggg ccgggaaacc cgcaacccaa ccgtcaacat
     4081 cccgcgggcc atcgtcgcca cggcgatctt tgccggcgtg ttcttcgtgt tgacgaccta
     4141 tctgatcgtc cagggctttg atgacgacgc cgccaagctt ggcgccagtg ccacaccgct
     4201 cgcggacatc accagcagca tcagcgtggg cctgaccgcg ctggtgtatt tcggcgccgc
     4261 gatcagcggc ttcgcctgcg cgctggcatc gctgaatgcc tacgggcgaa tgctgttctc
     4321 gctcggacgc taccagttcg ttcacacctc gatgggacta gtgcatcaca acttcaagac
     4381 accctacgtg gccttgacgt ccggtgcgtt gatcaacttc atcgtctgcg ccgcgtttgc
     4441 cggtcaggcg gaaactgaca cctttggctg gtacggcacc ctcgcttcct acggtttcat
     4501 catcgtgtac ttcctgtgct cggtgagcgc gccggtcttt ttggcgaaaa acggcggcgc
     4561 cacggccggc gacaaattcg tcggcggtct cggcgcggtg ctgatggtgc tgtcgctggt
     4621 cggcagcctc tatccggtgc cggcctcgcc atacaatctg tttccttatt tcttcctggt
     4681 ctacatcctg ctgggggtga gctggttctt cattctgaag gcgcgcgtcc cgcaggcgat
     4741 cctcggcatc gagcatgatc tggaaatagc ggcatcgacg cccgccgagt agtcgaggcc
     4801 gaatagagac agacggccgc gaccgctgcg aagcgccgcc gctgccatga cccaggggtt
     4861 gcttccccgg cgctacgacc cctgcgtctg ccacacccct tccgcttcgg gaggggtgtg
     4921 tgcttgccag cggtatacgc tcccaagata ctggtcagat ccgatgcgta agccgggcgg
     4981 ctgactggtc atgttcaggc tggcgcccct cagagcgttt tcaagcgaaa gcctgccccg
     5041 gacttgatcc ggggtggaaa ccggttcgcg ttaagaaaac gcgtcaaatc aagaatctag
     5101 agcctttatc cgtttcgatt ctatcgaaac ggataaaggc tctaggaggc cagcgaaatg
     5161 agccagcagc catcaacgct caatgcctcg ggatggacgc tcgcggtcgg agcatcttcg
     5221 tcggcttcgg caacgtccga gggcgcggat gtgtatgtca tccataaagg cgaagccttc
     5281 gacagaaaag tcttcgacag acccggccgc ggtatcatcg ccgccgtcgc acgcggcatc
     5341 ggcgccgggc cccacgcgcg cgaagcggca catatcgcgc ttcacggcct gctggagggt
     5401 tactttggcg cggcggcgac gcttggcccg gggcgtgccg ccggtcttgc gttgggaagt
     5461 gccaacgcct ggatgttcgg ccagtcccgc gccgatcccg ggcaccccat ggcggcttcg
     5521 ctgacggcgc tcatttttgt cggccggcgc gtccggatcg tccatgtcgg cgacggtcgc
     5581 gtttatcgcc gacgcggcag ccagctcact ccgctgacgt cggatcatgt gcacccggcc
     5641 gccgacggaa ccggcttgct gacgcggttg gtcggcggcg attccgagtt gcagatggat
     5701 tatctcgagg acgcctgcga accggccgac cgctatttca tattgagcaa aggcagcttt
     5761 gccgcgacgc cgtcggggcg gcttgccgat gtgctcacgg cgacgcgaac cgccgacgaa
     5821 gccgccggcc ttatcgtcgc gtcgatcccc gacaagaccg gggccagcgc cgcgaccgcg
     5881 atcgtgatcg acgttctcaa ggcgccggac tcaagcttcg atgaaatggc agcggcattc
     5941 ctgcggctgc cgatacgagg cgcgccgcgc gacggcgaga attgggatgg ttttttgctt
     6001 ggccgcacgc tctatcgcag ccgctacacc accctgaaac tggcccgcga caccctcggc
     6061 gacaaggacg ttgttctcaa gatacccctg ccctcgatgt tgcatgacga agtctttcgg
     6121 gccggattcc tgcgcgaggc ctgggtcggc gccaccgttc acagcccgcg aattgccagc
     6181 tatatcgagg tcgccccgga acgccgcagc agcctgtatc tcgtgctgcc atattatcac
     6241 ggggagacgc ttgaggcccg gctgtcaagg acggacccaa tcgcctatct cgaaggcgtt
     6301 gggcttgcgc taaaactttg cgcggcggtg caggatttgg cggcattgca aatcattcac
     6361 cgcgacctca agccggagaa cattcttctg cttcccgacg gcgagatcaa actgctcgac
     6421 ctcggcctcg cctatctgcc cggcgtcgac gagcccgacg acgatcgtct gggcggcacc
     6481 acccgctaca tggcgcccga gctgttccga aagcagccgg cggatcaacg ctccgaggta
     6541 ttttcgctgg gagtgacggt ctatcgtttg ttctccggcg gcccgtttcc gttcggccag
     6601 cgcgaaacta tcccgctgca aaggttacgt cccgacctgc ctgcctggct cggccagtgc
     6661 atgatgagcg cgatggaaat gaatcgcgaa aagcgtttca aggacgcaaa cgagtttgcc
     6721 agggcgctgg aaaatggctt gagccagggc gccgcccggc cgctcggcac cttgccgccg
     6781 cagcatcggc ggatcgatcc gttgcggctt tggcagggac tcacatttct gtttgccgcc
     6841 gggtttttcg tgctgctgat catgttgctg cgcctgcgat gacaggcatg caactccagc
     6901 acccaaaagc gagtgcgccc catgaagatt ttcaggacgg aaaatcggct tccttgacct
     6961 gaattctgtc ggcgtgaagc gaccagtggc gaagcgcctg cgtttcgcag aattttgtct
     7021 tcgccagttc cgtggcctgg tcctcgttcg aagcgtcaat ctccagagaa ccctgacaga
     7081 tttctgtctc tcggccgttc tcgccgagca cgtccttcat aaaacgaacg acaaatcgtg
     7141 acatggaacc tcccgtctgc gcccccagcg ccaaaacaga tccattacaa tcttatggtg
     7201 cggaaatcgt tttgaccgaa atcaagacct acgcggttgt ccgtaacgga agaccgcact
     7261 tccatgggat cacctgatct acgacccggc ggcttgctcc ccgagaaatg cacctctcgc
     7321 cgccgtcgtg attgcggtaa tgaggtcgat cacggcgcga acggcgggcg aacggccgat
     7381 gtcgcggtgg aacaggagcc atagctcccg ggtcaacggg ggtaatcgcg tcggaacgct
     7441 gaccagatcg gggacgccgc gtgccatcat gcagggaagc accgcaagcc ccaagccggc
     7501 acgtgcggcc gtcagcagga cggcaaggtc gttggagcgg agcgcaagg
//
LOCUS       DASWGM010001025         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1498785,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001025 DASWGM010000000
VERSION     DASWGM010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498785"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..1438)
                     /locus_tag="VGA15_27695"
     CDS             complement(335..1438)
                     /locus_tag="VGA15_27695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX9591514.1"
                     /translation="MRQFMLPDLGEGLEEAEIVTWHVNEGDHVVTDQPLVSVETDKAV
                     VEVPSPWSGRIARVFGAKGDLVKVGAPLVEFAEGAAQDTGTVVGQLDTGERQAAVAER
                     PAEPAPGRMLQVLPAVRALARKLDVDLNAVQATGPGGTITRADVERAAKSLAEAGPAE
                     ALRGMRRAMAQRMAAAHAEVVPATVIDEADIDEWRTGEDASVRLMRAIAAACRAEPAL
                     NAWYNSGAGVRRLVQRVDIGIAIDTEGGLIVPVMRNVVERDARDLRAGLDRLRADAIA
                     RSIPPEELRGATITLSNFGMIGGRFASLVVVPPQVAIVGAGRIAPLVVAHRGRPAVRR
                     VLPLSLTFDHRVVTGGEAARFMVALKSDLERTS"
     gene            complement(1458..>2300)
                     /locus_tag="VGA15_27700"
     CDS             complement(1458..>2300)
                     /locus_tag="VGA15_27700"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX9591515.1"
                     /translation="RFGPERVFDTPLAELLISGLCVGMAAQGLKPVGEIQFMGFIYPC
                     IDQLVNHASRLRNRTQGRLSCPMVLRTPHGSGIRAPEHHSESTEAMLAHIPGLRVVIP
                     SSPERAYGLLLAAIRDPDPVVFLEPTRIYRAARGEVEDNGEALPLDVAFILREGRDVT
                     LISWGAMVKETLAAAETLDAEGISAEVIDLATLKPYDEETLLSSVAKTGRCVIVHEAA
                     RTGGFGAEIAALIAERGLTSLLAPVARVTGYDTVMPLPRLEQHYMPSVDRIATAGRKA
                     CQFS"
BASE COUNT          410 a          760 c          715 g          415 t
ORIGIN      
        1 gccttggcgc cgtgcgcttc cagcaactgt cgcgcatagt catggatttc gatttctcgc
       61 atggcgtcat gctcctctcg ttctgcgcgc gaccgtgaac tccagtcggg tagcagggcc
      121 gtagacgttg acacccatca agcccatgca gtctgcatcc tggcgcggga cgtttgcaag
      181 agcctcttgc agatcatggg ccgccaaagt cgggccaccg gcaaccattc cacgacggga
      241 atgccgtaat gcttccgccc gagttgtgag ctttcagaag catcgtgtct ttccaaggcc
      301 agatcaccgt ttccggctca cgcgtgtcgc ggttctagga agttcgctca agatccgact
      361 ttagcgcaac cataaagcga gctgcctcgc cgcccgtcac aacgcgatgg tcaaaggtga
      421 gcgataacgg cagcacgcgt cgtaccgccg gtcggccccg gtgtgccacc accagggggg
      481 caatccggcc ggcaccgacg atcgccacct gtgggggcac aaccacgaga ctggcgaagc
      541 ggccgccgat catgccgaag ttcgacagcg tgatggtcgc gccccgaagt tcttccggcg
      601 gaatcgagcg ggcgatcgcg tcggcccgca gcctgtcgag cccggctcgc aggtcccgtg
      661 cgtcgcgctc gacgacgttg cgcatcaccg gcacgatgag gccgccctcg gtgtcgatcg
      721 cgatgccaat atcgacgcgc tgaaccagac gtcgcacgcc cgcgccggaa ttgtaccagg
      781 cgttgagcgc gggttccgcc ctgcaggcag ccgcaatcgc gcgcatcagt cggacacttg
      841 cgtcttcgcc cgttcgccat tcgtcgatat cagcttcgtc gatgacagtg gccggaacga
      901 cttcggcatg cgccgctgcc atgcgctggg ccatggcgcg tcgcatcccg cgcaatgcct
      961 cggccggacc cgcctcggcg agacttttgg cggcccgctc gacgtcggcc cgtgtgatgg
     1021 tgccgccggg tccggtggcc tgtacagcgt tgagatcgac atcgagcttg cgtgcgaggg
     1081 cgcgcaccgc cgggagcacc tgaagcattc gccctggcgc cggctcggca ggtctttcgg
     1141 ccactgcagc ttgcctctcg ccggtgtcga gctggccgac cacggtgcca gtatcctgcg
     1201 cggcgccctc ggcgaactcg accaggggcg caccaacttt caccagatca cctttggccc
     1261 cgaacacccg cgcgatgcgg ccactccacg gtgacggcac ttcaaccacg gccttgtcgg
     1321 tctcgaccga gacgagcggt tgatccgtaa cgacgtgatc cccctcgttc acatgccagg
     1381 tcacgatctc ggcctcttcg agaccttcgc caagatcggg caacatgaac tgccgcatcg
     1441 ggtgtgttcc gctgatctca actgaactgg caagccttgc gtccggcggt cgcgatacga
     1501 tcgaccgacg gcatataatg ctgctcaagc cggggcagcg gcatcaccgt atcatagcct
     1561 gtgacgcggg cgacaggagc gagaagagac gtcagcccgc gctccgcgat aagcgcggcg
     1621 atctcggcac cgaagccgcc ggtccgtgca gcttcgtgca cgatcacgca gcgtccggtt
     1681 tttgccacgg agcttagcaa ggtctcctcg tcatagggct tgagggtcgc aagatcgata
     1741 acctccgcgc tgatgccttc agcgtcgagc gtctcggcag cagccaaagt ctccttcacc
     1801 atcgcgcccc agctgatcag cgtgacgtcc cggccttccc tgagaatgaa agcgacgtcg
     1861 agcggcaagg cctcgccgtt gtcttcaact tcgcctctcg ccgcgcggta gatgcgcgtc
     1921 ggttccagga acacaaccgg atcggggtcg cggatggcgg cgagcagcag gccgtaagcg
     1981 cgttcgggcg aagatggaat gaccacgcgc agccctggaa tatgggcgag catggcctcg
     2041 gtgctctcgg agtgatgttc gggagcgcgg atgccactcc catgcggggt gcgtaaaacc
     2101 atcggacaac tgagccggcc ttgcgtgcgg ttgcgcagac gcgaggcatg gttcaccaat
     2161 tgatcgatgc agggataaat aaatcccatg aactggattt cgccgaccgg cttcagtcct
     2221 tgtgccgcca tgccgacgca gagtccgctg ataaggagtt cggcaagcgg cgtatcgaac
     2281 acgcgttccg gtccgaaacg
//
LOCUS       DASWGM010001026         6672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1095879,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001026 DASWGM010000000
VERSION     DASWGM010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095879"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGA15_27705"
     CDS             complement(<1..224)
                     /locus_tag="VGA15_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9591516.1"
                     /translation="MAEFDVDLFVIGGGSGGVRAARIAAGYGARVMVAEEYRMGGTCV
                     IRGCVPKKLFVIGSHIQHEIEDAAGFGWLIP"
     gene            complement(308..829)
                     /locus_tag="VGA15_27710"
     CDS             complement(308..829)
                     /locus_tag="VGA15_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEX9591517.1"
                     /translation="MKRLFRILSAAGLVMGLALAGVPAGAQQAPALKPASPAAIAAAK
                     EILTMKNASAMYANAIPGIVQQTKQVLLQNNLNYQKDLNEVAVIVAQGLAGREQEIGE
                     GMAKIYANEFTEQELKELVTFYKSPLGQKLLASEPRAIQFSMSYMNQWAQSFAEVVNG
                     QFRAEMRKRGKEI"
     gene            complement(853..1551)
                     /gene="rpiA"
                     /locus_tag="VGA15_27715"
     CDS             complement(853..1551)
                     /gene="rpiA"
                     /locus_tag="VGA15_27715"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX9591518.1"
                     /translation="MSMDELKRQAAGRALEFVADGMKLGLGTGSTAKHFVELLGERVR
                     AGLNVIGVPTSEATRADAVRCGIPLTTLDDIDRLDLTVDGADEIDSGLNLIKGGGGAL
                     LREKIVATASDRMIVIADDSKWVEVLGRFPLPIEVIPFGLAATRRAIGRAFAESGVSG
                     QMVVRKAKDGHVFVTDGGHWIVDAHLERISDAPRLARLLNSIPGVVEHGLFIGLASTA
                     MLAGSQGIRVFERR"
     gene            1789..2475
                     /locus_tag="VGA15_27720"
     CDS             1789..2475
                     /locus_tag="VGA15_27720"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX9591519.1"
                     /translation="MTSPRTVVFDLDGTLVDTAPDLINALNYVLDREGLPPVPLHAAR
                     NMIGAGARRLIERGLELEGRSVGPEDITRLTSDFIDYYAAHIADASRPFDGLESALDD
                     LTARGYRFAVCTNKLEWLSKLLLDQLGLSSRFSAICGADTFGVSKPDPAILQQTIARA
                     GGQLSSAVMVGDAGPDIGVARRAGVPVIGVEFGYTDVPIADLKPDLLINHMRNLPAAV
                     ERLMYPRNST"
     gene            2629..3093
                     /locus_tag="VGA15_27725"
     CDS             2629..3093
                     /locus_tag="VGA15_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591520.1"
                     /translation="MRRAIAIAITGYGLAGCSSLPSLDAFKPTPPTVQVQLDSVPPGA
                     DARTSLGPGCKTPCAVAVSAPDSGFSVAYTLNKFQPATVPVQVIRIPGDFSSPVSTTI
                     DPNPVVAELQPAAPPPRAARKMLTPKKPKPPKAAAAPAGAPEPSPTPAPTTR"
     gene            3229..4263
                     /gene="moaA"
                     /locus_tag="VGA15_27730"
     CDS             3229..4263
                     /gene="moaA"
                     /locus_tag="VGA15_27730"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX9591521.1"
                     /translation="MNGPLISPPTSLSRPMTDPFGRTISYLRVSVTDRCDLRCFYCMS
                     EDMTFLPKADLLTLEELDRLCTAFIAKGVRKLRLTGGEPLVRRNVMSLVRSLSRHLDA
                     GALKELTLTTNGSQLARFASELRDCGVRRINVSLDTLDPAKFRAITRWGDLDKVLAGI
                     EAARSAGLAVKINAVALKNLNEEEIPSLMQWAHGKGMALTLIEVMPMGDIGEGRIDQY
                     VPLSLLRARLAKQYTLTDLDEDTGGPARYVRVSETGGKLGFITPMTHNFCESCNRVRI
                     TCTGTLHTCLGHEDASDLRKPLRASADNDLLAAAIDRAIGLKPKGHDFIIDRRHNRPS
                     VSRHMSVTGG"
     gene            4455..5036
                     /locus_tag="VGA15_27735"
     CDS             4455..5036
                     /locus_tag="VGA15_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX9591522.1"
                     /translation="MQSLLKMSRGIDAFTRWTGKRLAWLILVAVVVSALNAIVRKSFD
                     TSSNSWLELQWVLFSIVFLLCSSWTLLDNEHIRIDIVNNMLPKQVRNSIDLIGHAFFL
                     IPLTVVMIITGGPFFMRSVEINEQSGNAGGLPQWPAKSLIIIGFTLLLVQGISELIKR
                     IAVMRGLIPDPHASQVNALEAEVGHLVEAIKKD"
     gene            5066..>6672
                     /locus_tag="VGA15_27740"
     CDS             5066..>6672
                     /locus_tag="VGA15_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX9591523.1"
                     /translation="MAAFLIANMAPLMFASLVVVLLLGYPAAFSLGAVGLIFAFVGIQ
                     LGEFRPDFLQALPERVYGVMNNDTLLAIPFFTFMGLVLERSGMAEDLLDTIGQLFGTI
                     KGGLAYAVVFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRMASGIIAASGTLA
                     QIIPPSLVLIVMADQLGKSVGDMYEGAFVPGLVLAGLYALYAFFVTLIFPKAAPGLPA
                     EAIGFREKDGSRGLISLLMLFIASCFFGWFMMRNSETHGADFVVLSMFFGILFAFFVA
                     VVNWVIDKFTGFRFLSAMAQQTTFVMVPPLFLIFLVLGTIFIGLATPTEGGAMGASGA
                     IILGAAKRRLSWDLIRQATESTAKLSAFVVFILVGARVFSLTFYGVNGHVWVEHLLTS
                     LPGGQIGFLIFVNAFVFVLAFFLDFFELAFIVIPLLGPAAEHLGIDLIWFGVILGVNM
                     QTSFMHPPFGFALFYLRSVAPKDSYIDRVTGKRMDPVTTGQIYWGAVPFVVIQVAMVL
                     LVIMFPSMVMHYKGALSTIDPNTIKIE"
BASE COUNT         1169 a         2158 c         1966 g         1379 t
ORIGIN      
        1 gggatcaacc agccaaagcc cgcggcgtcc tcgatctcgt gctggatatg cgatccgatc
       61 acgaacagct ttttcggcac gcagccgcgg atcacgcagg tgccgcccat ccggtattcc
      121 tcggcaacca tcacccgggc gccatagccc gcggcgatcc gggcggcgcg aaccccgccc
      181 gaacctcctc cgatgacgaa cagatcgacg tcgaactcag ccatatccac cccgagcgaa
      241 atcggattct ttggatcaga tttccacttc agatttgcac atcagatttg cacatcagat
      301 ttacacgtca gatttccttg ccgcgcttgc gcatctcggc gcggaactga ccgttgacga
      361 cctcggcaaa gctctgcgcc cactggttca tataggacat gctgaattgt atcgcccggg
      421 gctcgctcgc cagcagcttc tggccgagcg gcgacttgta aaacgtgacc agctccttca
      481 gctcctgctc ggtaaattcg ttggcgtaga tttttgccat gccctcgccg atttcctgct
      541 cgcgaccggc caggccctgg gcaacaatca cagcgacttc attgagatcc ttctggtagt
      601 tgaggttgtt ctgcagcaaa acctgcttgg tttgctggac gatgccggga atggcgttcg
      661 catacatggc gctggcattc ttcatcgtca gaatttcctt ggccgccgcg atcgccgccg
      721 gagatgccgg cttgagcgca ggtgcctgct gggcgcctgc gggaacgccg gccagagcca
      781 gtcccatcac aaggcccgcg gccgacaaga ttctaaaaag gcgcttcatt cctagtctcc
      841 tcgattgcgg cgttaccgcc gttcaaaaac acgaattccc tgagagcccg ccagcatcgc
      901 cgtgctggca aggccgatga acagcccatg ctcgacaacg cccggtatcg agttcagcaa
      961 tcgcgcgaga cgcggagcat cgctgatgcg ttcgagatgg gcatcgacaa tccagtggcc
     1021 gccatcggtg acaaaaacgt ggccgtcctt ggcctttcgg accaccattt gcccggaaac
     1081 gccgctttcc gcaaatgccc ggccgatggc ccgccgcgtc gcggccaggc cgaacggaat
     1141 gacctcgatt ggtaacggaa agcggccgag aacctcgacc catttggaat catcggcgat
     1201 cacgatcatg cgatcagacg ccgtggccac gatcttttcg cgcagcaatg cgccgccgcc
     1261 gcccttgatg aggttgagcc cggaatcgat ttcgtccgcg ccatcgaccg tcagatcgag
     1321 ccgatcgata tcgtccagcg tggtcagcgg aatgccgcac cgtaccgcat cggcgcgggt
     1381 cgcctccgac gtcggcacgc cgatgacatt gagcccggcg cgaacccgct cgcccagcag
     1441 ctcgacaaaa tgcttcgcgg tcgaaccggt gccgaggcca agcttcatgc cgtcggccac
     1501 gaactcgagc gcgcggcccg ccgcctgccg tttcaattcg tccatgctca cgtgcaaatg
     1561 atgccccggg gactccgatg ccgattatgc gaccggcgat gtctggcggg catatctagc
     1621 gtcgtttcgc ggggaggaac agcaccatac gcctgatttg gccgcaattg acgactttcg
     1681 cgcctggcga gccgcgcatg gggcggattg tccccgcttt tgcgcaagta tcacagtcac
     1741 ctgtcgtccc accttgcatg attgcacgag gaccgatagc ggttttcgat gacctctccc
     1801 cgcactgttg ttttcgatct cgacggcacg cttgtggata cggcgcccga cctgatcaac
     1861 gcgctcaact atgttctcga ccgcgagggc ctgccgccgg taccgctgca tgccgcgcgc
     1921 aacatgatcg gcgcgggcgc ccgccggctg atcgagcgcg gcctcgagct ggagggccgg
     1981 tccgtcggcc ccgaagacat cacccggctc accagcgatt tcatcgatta ttatgccgct
     2041 cacatcgccg acgcctcgcg cccgttcgat ggcctggaaa gcgcgctcga cgatctcaca
     2101 gcgcgcggat atcgctttgc ggtttgcacc aacaagctcg aatggttgtc gaagctcctg
     2161 ctcgaccagc tcggattgag ctcgcgattt tccgcgattt gcggcgccga cacgtttggc
     2221 gtctcaaaac ccgacccggc gatcttgcag cagaccatcg cgcgcgcggg cggccagctg
     2281 tcctctgcgg tcatggtcgg cgatgccggt ccggatatcg gcgtggcgcg gcgcgccgga
     2341 gtgccggtga tcggcgtcga atttggatac accgacgttc ccatcgccga cctcaagccg
     2401 gatctgctca tcaatcacat gaggaacttg ccggctgcgg tggagcgcct gatgtatccg
     2461 cggaattcca cctaagccat tgattcactt agtatatcaa aatcgctagt tacgcgcgta
     2521 aaccattact taaccagtat atccccacgg ttgcacggct ggtgtgacgc acctatgctc
     2581 atccttgggg gctgtggcag gtagccgcac taacgtggcg gattgatcat gcgtcgtgct
     2641 atcgcgatag caataaccgg gtacggcctg gccggctgct cttcgcttcc ttccctggat
     2701 gccttcaagc cgacgccgcc aacggtgcaa gtgcagctcg attcagtgcc gccaggcgcc
     2761 gatgcgcgga cgtcgctggg gccgggttgc aagaccccgt gcgcggtcgc ggtgagcgcg
     2821 cctgacagcg gtttctcggt cgcctacacg ctgaacaagt tccagccggc gaccgtcccg
     2881 gtacaggtca tccgtatccc cggagatttc tccagtccgg tgtcgaccac gatcgatccc
     2941 aacccggttg tcgcggaact tcagccggcc gccccgccgc cgagggccgc ccgcaagatg
     3001 ctaacgccga aaaagccaaa accgcccaag gccgccgcgg ccccagcggg agcgccggag
     3061 ccgtcgccga cacccgcccc gaccacccgc taaatgcttg cactgcagca gaaaaggtgc
     3121 ggattgtatg cgcctcgcat catgcataga ttgcgatcgc ggaaccgtcc gccctatcca
     3181 ttccgcctta tccatcatgg cggctgccac agtaacaagg cgcattgaat gaacggaccc
     3241 ctgattagtc ctcccacgtc gctgtcgcgt cccatgaccg acccgttcgg ccggaccatc
     3301 agctatttgc gggtatcggt cacggatcgc tgcgatctgc gctgctttta ctgcatgtcc
     3361 gaagacatga catttctgcc caaggccgat ctgctgacgc tcgaggagct cgatcggctt
     3421 tgcacggcgt tcatcgccaa gggcgtgcga aagttgcggc tgaccggcgg cgagccgctg
     3481 gtccggcgca acgtgatgtc gctggtgcgc tcgctgtcgc gccacctcga tgccggcgcc
     3541 ctcaaggaac tgaccttgac gaccaatggc tcgcagctgg cgcgttttgc gagcgaatta
     3601 agggactgcg gagtacgccg catcaacgtg tcgctggata ccctcgaccc cgccaaattc
     3661 cgcgccatca cccgttgggg cgatctcgac aaggttctgg ccggcatcga ggcggcgcga
     3721 tcggcgggtc tcgccgtcaa gatcaacgcg gttgcgctga agaatctgaa cgaggaagaa
     3781 attccttcgc tgatgcaatg ggcgcacggc aagggcatgg cgctgacgct gatcgaggtc
     3841 atgccgatgg gcgacatcgg cgaaggacgc atcgatcaat acgtcccgtt gtccctgctc
     3901 cgcgcgcgcc tcgccaagca atacaccttg accgacctcg acgaggacac cggcggcccc
     3961 gcacgctacg tgcgcgtcag cgagaccggc ggcaagctcg gcttcatcac gccgatgacc
     4021 cataatttct gcgaatcctg caatcgggtt cggatcacct gcaccggcac gttgcacacc
     4081 tgtctcggcc acgaggatgc gtccgatctg cgcaagccgc tgcgggcctc cgccgacaat
     4141 gacctgctcg ctgccgcgat cgaccgcgcc atcggcctga agccgaaggg ccacgacttc
     4201 atcatcgatc gccggcacaa ccgcccgagc gtcagccgcc acatgagcgt caccggcggc
     4261 tgaggcttgc gccccggccc ccgcgacggt cgtccgcggg gccaaaatcc ccttcattat
     4321 caacaaactt ccaattgacg gcggcgccac gcgctggaat ggtgcggcgg cttcatgcta
     4381 gctcggctgg ataagccgct gcgcctgacg ggcgcagtca agacggcagg ggcgcaatcg
     4441 ggggaggact atcgttgcaa tcgctcttga aaatgagccg cggtatagac gcgttcacac
     4501 ggtggacggg caagcggctg gcgtggctga tcctggtcgc cgtggtggtg tccgcgctca
     4561 atgcgatcgt gcgcaagagc ttcgacacct cgtccaattc ctggctcgag ctgcaatggg
     4621 tgctgttcag catcgtgttc ctgctgtgct cgtcctggac cctgctcgac aacgagcata
     4681 tccggatcga tatcgtcaat aacatgctgc cgaagcaggt gcgcaacagc atcgatctta
     4741 tcggtcatgc gttttttctg atcccgctca ctgtcgtcat gatcatcacc ggcggtccgt
     4801 tcttcatgcg ctcggtcgag atcaacgaac aatccggcaa cgcggggggc ctgccgcagt
     4861 ggcccgccaa gtcgctgatc atcatcggat tcaccttgct gctggtgcag ggcatatccg
     4921 aactgatcaa gcgcattgcg gtgatgcgcg ggctcattcc ggacccgcac gcatcgcaag
     4981 tgaatgctct cgaggccgag gtcggacatc tcgtcgaggc gatcaagaaa gactgatcgc
     5041 ggacgcggtc gcagggggat tctggatggc tgcttttctg atcgccaata tggcgccgct
     5101 catgttcgcg tcgctggtcg ttgtcctgct gctgggttat ccggcggcgt tctcgctcgg
     5161 cgcggtggga ctgatctttg ccttcgtcgg cattcaactt ggcgaattcc ggcccgactt
     5221 cctgcaggcg cttcccgagc gcgtctatgg cgtgatgaac aacgatacgc tgctcgcgat
     5281 accgttcttt acgttcatgg gactggtgct cgaacgatcc ggcatggcgg aggacctgct
     5341 cgacaccatc gggcagctgt tcggcaccat caagggcggc cttgcctatg cggtggtgtt
     5401 cgtcggcgcg ctgctcgccg ccaccacggg cgtggtcgcg gcttccgtga tttcgatggg
     5461 cctgatctcg ctgccgatca tgttgcgcta cggatacgac cgccggatgg cttccggcat
     5521 tatcgcggcc tcaggcacat tggcgcagat cattccgccg tcgctggttc tgatcgtgat
     5581 ggccgaccag ctcggcaagt cggtcggcga catgtacgag ggcgccttcg ttccgggatt
     5641 ggtgctcgcg ggcctctatg ccttgtacgc gtttttcgtc acgctgatat ttcccaaggc
     5701 ggctccgggc ttgccggctg aagcgatcgg ctttcgcgag aaggacggca gccgcggatt
     5761 gatttcgctg ttgatgctct tcatcgccag ctgtttcttc ggatggttca tgatgcgaaa
     5821 ctccgaaacg cacggcgccg atttcgtcgt gctcagcatg ttcttcggca tcctgttcgc
     5881 gttcttcgtc gctgtcgtga actgggtcat cgacaagttc acgggattcc ggtttctgtc
     5941 cgcgatggcg cagcagacga ctttcgtgat ggtgccgccg ctgtttctga tttttctggt
     6001 gctcggcacg atcttcatcg ggctggccac accgaccgag ggtggcgcga tgggcgcctc
     6061 cggcgcgatc attctcggcg ccgccaagcg ccggctgagc tgggatctga tccggcaggc
     6121 cacggaatcg acggcgaaac tgtccgcctt cgtggtcttc attctggtcg gagcgcgggt
     6181 tttctcgctg accttctatg gcgtcaacgg tcacgtctgg gtcgaacatc tcttgacctc
     6241 cctgcccggc ggtcagatcg ggtttctcat attcgtcaac gccttcgtgt tcgtgctcgc
     6301 cttcttcctc gatttcttcg agcttgcctt catcgtcatt ccgctgctcg gaccggccgc
     6361 cgaacacctc ggcatcgacc tgatctggtt cggcgtcatc ctcggcgtca acatgcagac
     6421 ctcgttcatg catccgccgt tcgggttcgc gctgttctac ctgcgctcgg tggcgccgaa
     6481 ggattcctac atcgaccgcg tcaccggcaa gcgcatggac cccgtcacca caggccagat
     6541 ctattggggt gcggtgccgt tcgtcgtgat ccaggtggcc atggtgctgc tggtgatcat
     6601 gttcccgtcg atggtgatgc attacaaagg ggcattatcc acgatcgatc ccaacaccat
     6661 caagatcgag at
//
LOCUS       DASWGM010001027         4729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_675145,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001027 DASWGM010000000
VERSION     DASWGM010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675145"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VGA15_27745"
     CDS             <1..1545
                     /locus_tag="VGA15_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX9591524.1"
                     /translation="FFALTIIIGVVAWLFVLAQQSRRAAEEETRRQTALLIQEIDAHK
                     RTDAELQRAKEVAESANLAKSRYVVGLSHELRSPLNAISGYAQLLEQDSSLLPKPRDQ
                     VRVVRRSADHLSGLIDGILDISKIEAGRLYLSRDEVRLSEFLDQLVGMFRLQAAAKGI
                     DFIFKRPTVLPVVVFADEKRLRQILINLLSNAIKFTQRGSVQFVVHYRSPVAEFEVID
                     SGPGIQATDLERIFAPFERGALGVSQPQTGTGLGLTISRLLAGVMGGDIQVASAVGTG
                     STFRVKILLSEVTHPTRIAPVDAPFFGYHGPRKTILITDDDPVQRDLLREVLTPLGFI
                     LLSAPDGPGCLALAQHCRPDLFLLDISMSGMDGWTVAETLRSTGHRQARILMVSASAL
                     EAHGTPLAQPFHDGYLMKPIDIPRLLELIRQLLRIEWQYETDQVAVPHWKPDSGSRPP
                     VKHVEELIGLGQMGYIRAIQVKLDEIGTDYPEHTDFVSQMRILIDRFDLDQYMATLKT
                     LHSYDH"
     gene            1535..2467
                     /locus_tag="VGA15_27750"
     CDS             1535..2467
                     /locus_tag="VGA15_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9591525.1"
                     /translation="MITESKKRDVALVIDDSPETLRLLTDALDGAGMTVMVAMDGAAA
                     MRIVEQITPDIVLLDAVMPGMDGFETCRRLKRDAGLSNVPVIFMTGLAETEHIVRGLE
                     AGGVDYVTKPIVIEEMLARIRVHLANARLTQSARAALDVSGRYLLAVSRQGKIMWATP
                     QAQKLLSDKLAAAGADDELALPQPMLEWLEQAQKGKAGSKTTAMASFPDDEQLRLQYM
                     GKLGPNEFLLRLAKDTSTHLPAEFSSELGLTTREGEVLSWLSKGKTNRDIAQILGLSP
                     RTVDKHLEQIYAKLGVENRTAAAAIATSTSRRNS"
     gene            2760..3560
                     /locus_tag="VGA15_27755"
     CDS             2760..3560
                     /locus_tag="VGA15_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEX9591526.1"
                     /translation="MNLDAYRIVSEPFYQPVGDEIAVFEAAFSERLPVMLKGPTGCGK
                     TRFVEHMAWRLNKPLVTVAAHEDMTASDLAGRYLLEPDGTVWHDGPLTMAVRYGAICY
                     LDEIVEARQDTTVVIHPLTDARRILPLEKHNEIVPAHVDFQLTISYNPGYQSAVKDLK
                     ESTKQRFLAIDFGYPPPAVEAAIVAREAGTNVDLAGTLVAIAERSRNLQGHGLDEGAS
                     TRMLIHAGRLVRAGLPLEAAVRSSIVLPITDNHDIREALSAAIEACIP"
     gene            3557..>4729
                     /locus_tag="VGA15_27760"
     CDS             3557..>4729
                     /locus_tag="VGA15_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591527.1"
                     /translation="MSAALRSASLRLISAQPEGAVRRVHLLMRQRDALRPLFRIVWDN
                     LAARFDADELEGWAAGVLALADVNAGPSCLVAFWELSRNQPTEEGIAPLIVAAHTAAN
                     VCRHAGAQGAAATIVSAAVARRILGADPSLARWWRTMDDLALRAPESVEAAASRMGEI
                     LSAGNIHAFESFVAAGLKLAAGDRRKRLAFFSLQDELARRLVARATAGVGFAEAERQT
                     KAFVTSLWGRPPMLRSFPSDTGQAPQRRAGIAGPLIRLPEIYRGVQGDAAYTLYGAAA
                     AHAQAHLVFGGARFPVGSLKSLQIALVTLIEDARIELLAMRQLPGLRKLWSPYHVAVA
                     AGVATAPTLLARLAKALFDRAYEDDDSFVAKGRSMFEARLPRLEDPAISRETGMLLG"
BASE COUNT          870 a         1522 c         1486 g          851 t
ORIGIN      
        1 ttcttcgcgc tgacgattat cattggcgtc gtcgcctggc tgttcgtgct ggcacagcag
       61 agccggcgcg cggcggaaga agaaacccgg cggcagactg cgctgctgat ccaggaaatc
      121 gacgcccaca agcgtaccga cgccgagttg cagcgcgcca aggaagtcgc cgaatcggcc
      181 aatctcgcca agagccgata tgtcgttggg ctaagccatg aactgcggtc gccgctgaat
      241 gccatctccg gctacgctca attgctggag caggattcca gtctgctgcc caaaccgcgc
      301 gatcaggtgc gcgtggtacg ccgcagcgcc gatcatctgt ccgggctgat cgatggtatt
      361 ctcgacattt cgaaaatcga ggcgggccgg ctgtacctgt cgcgggacga ggttcgcctg
      421 agcgaattcc tcgatcaact cgttggaatg ttccgtctcc aggccgctgc caagggcatc
      481 gatttcatct tcaagcggcc gaccgtgttg cccgtcgtgg tattcgccga cgaaaagcgg
      541 ctgcgccaga tcctgatcaa tctgctgtcg aacgccatca aattcacgca acgcggcagc
      601 gtgcaattcg tcgttcacta tcgcagtccc gtcgccgagt ttgaagtgat cgatagcggt
      661 ccggggattc aggcgaccga cctcgagcgg atcttcgcac cgttcgagcg cggcgctttg
      721 ggcgtctcgc agccgcagac cggaaccggt ctcggcctga ccatcagccg actacttgcc
      781 ggcgtgatgg gcggcgacat ccaggtggcc agcgccgtcg gaaccggcag caccttccgg
      841 gtgaaaatcc tgctgtcgga agttacccat ccgacgcgaa ttgcgccggt ggatgctccg
      901 ttctttggct accatggtcc tcgcaagacg atcctgatca ccgacgatga tcccgtacag
      961 cgcgatctgt tgcgcgaagt gctgacgccg ctgggcttta tccttctgag cgcgcccgac
     1021 ggacccggct gtctcgcgct cgcccagcat tgccggcccg atctgttcct gctcgacatc
     1081 tcgatgtcgg gcatggacgg ctggaccgtc gcggagacgc tgcgttcgac cggccatcgc
     1141 caggcccgca tcctgatggt ttcggcaagc gcattggagg cgcatggcac gccgctagcg
     1201 cagccgtttc acgatggcta tctgatgaaa ccgatcgata tcccgcggct actggaattg
     1261 atccgccagc tgctgcggat cgaatggcaa tatgaaaccg atcaagttgc cgtgccgcac
     1321 tggaagccgg acagcggctc acggccaccg gtaaagcatg tcgaggaact catcggtctt
     1381 ggacaaatgg gttacattcg cgcgattcag gtgaagctcg atgagatagg aaccgattat
     1441 cccgagcata ccgacttcgt ctcgcaaatg cgcatcctga tcgaccgctt cgacctcgat
     1501 cagtacatgg caaccctgaa gacactgcac agctatgatc actgaatcca agaagcgcga
     1561 cgtcgcgctc gtcatcgacg attcgccgga aacgctgcgg ctgctgaccg acgcgctcga
     1621 cggcgccgga atgacggtca tggtcgcgat ggacggcgcc gcggcgatgc ggatcgtcga
     1681 acagatcacg ccggacatcg ttctgctgga cgccgtcatg cccggcatgg acggctttga
     1741 aacatgccgg cgtctcaagc gcgacgccgg cttgagcaat gtgcccgtca tcttcatgac
     1801 cggcctggcg gagaccgaac acattgtgcg cggccttgaa gcaggcggcg tcgattacgt
     1861 gaccaagccc attgtcatcg aggaaatgct ggcgcggatc agggttcatc ttgccaatgc
     1921 caggctgacc caaagcgccc gcgcggcgct cgacgtgtcc gggcggtatc ttctcgcggt
     1981 aagccggcaa ggcaagatca tgtgggcaac accgcaggcg cagaaattac tgtcggataa
     2041 gctcgccgcc gccggggccg acgacgaact cgcgctgccg cagccgatgc tggagtggct
     2101 cgagcaggct caaaagggca aggccggatc gaaaaccacg gccatggcgt cgtttccgga
     2161 cgacgagcag ctgcggctgc agtatatggg caaacttggt ccgaatgagt tcctgttgcg
     2221 gctggcaaaa gataccagca cccatctgcc cgcggaattt tccagtgaac tcggattgac
     2281 cacccgtgag ggcgaggtgc tgtcgtggtt gtccaagggg aaaaccaatc gggacatcgc
     2341 gcagatcctc ggcctcagcc caagaacggt ggataagcat ctcgagcaaa tctacgccaa
     2401 actcggcgtc gagaaccgca cggccgccgc cgccatcgcc accagcacga gccggcgcaa
     2461 ttcataaatc aggaattgcg gtcgccgagg ttcggaaaac gacttcgcgg cccctgcccg
     2521 cctcagaacg cagccgttcc gggtgcgcaa gctcattacc gtccaatcct ggccaaggtc
     2581 acttgtgcaa gatcgcgatt ggcagtctaa tgatgattga gacgtttgac gaacgtctgc
     2641 ttgcgcgctc cgaggctgtg gcgcgcccat aaggcgatgc ttgccgaacc cactgcggtg
     2701 ggaaggaggt cttcgcgcgg gcgggcggcc ttccccacgc acaatccgtg gagatgcgcg
     2761 tgaacctcga tgcctaccga atcgtatcag aaccctttta tcagcctgtg ggcgacgaga
     2821 tcgcggtgtt cgaggcggcc ttcagcgagc gtctgcccgt catgttgaag ggaccgaccg
     2881 ggtgtggcaa aacccgattt gtcgaacaca tggcctggcg tctgaataag ccgctggtga
     2941 ccgtcgccgc gcatgaagac atgaccgcct ccgaccttgc cggccgctat ttgctggagc
     3001 cggacggcac cgtctggcac gatggcccgc tgaccatggc ggtcagatac ggcgcgatct
     3061 gctacctcga tgaaatcgtc gaagcccggc aggacaccac ggtcgtgatc catccgctga
     3121 cggatgcgcg gcggatcctg ccgctggaaa agcacaacga gatcgtgccg gcgcacgtcg
     3181 atttccagct tacgatctcc tacaaccccg gctaccagag cgcggttaag gatctgaagg
     3241 aatcgaccaa gcagcgtttc ctggcgatcg atttcggcta tccgccgccg gccgtcgaag
     3301 ccgccatcgt ggcgcgcgag gctggaacca atgtcgacct tgccggaacc ctcgtcgcca
     3361 tcgcggagcg gtcccgcaat cttcagggcc acggcctgga cgaaggcgcc tcgacccgga
     3421 tgctgatcca tgcgggacgg ttggtgcgcg ccggcctgcc gcttgaagcc gcggtccggt
     3481 cgagcatcgt cctgccgatc accgacaacc acgatatccg cgaagcgctc agcgcggcca
     3541 tcgaggcctg cataccgtga gcgccgccct ccgatcggcg tcgctgcggc tgatttccgc
     3601 gcaaccggag ggcgccgttc gccgcgttca tttgctgatg cggcagcgtg atgcgctccg
     3661 gccgctgttt cgcatcgttt gggacaatct cgccgcgcgc ttcgatgccg acgagcttga
     3721 gggctgggca gccggcgtgc tggcgctggc cgacgtcaat gccggcccat cctgcctggt
     3781 cgcgttctgg gagctcagcc gaaaccagcc gaccgaagaa ggcatcgcac cgctcatcgt
     3841 ggccgcgcac acggctgcga atgtttgccg ccacgccggc gcacaggggg ccgccgccac
     3901 catcgtctcc gccgccgtcg cgcgccgaat tctgggcgcc gacccgagcc tcgcgcgctg
     3961 gtggcggacc atggacgacc tcgcgctgcg ggcgccggaa tcggtcgagg cggcggcatc
     4021 acggatgggc gaaatcctgt cggcgggcaa catccatgcc ttcgagagct tcgtcgcagc
     4081 cggcttgaag ctggcggccg gcgacaggcg taaacggctg gccttcttct cgctgcagga
     4141 tgagcttgcg cggcgactgg tggcaagagc aacggccggc gtcggattcg ccgaggccga
     4201 gcgccagacc aaggctttcg tcacctcatt gtggggccgg ccgccgatgt tgcgcagttt
     4261 tccaagcgac accggccagg cgccacaacg ccgtgccggc atcgcaggac cgctgatccg
     4321 cttgcccgag atctatcgcg gcgtgcaggg cgacgccgcg tacactttat atggagccgc
     4381 ggcggcgcac gcccaggcgc atctggtgtt cggcggcgcc cgcttccccg tcgggagcct
     4441 aaaatcgctt cagatcgcgc tcgttacctt gatcgaggat gcccgcatcg agttgctggc
     4501 gatgcgccag ctgccgggat tgcgtaaatt atggtcgccc taccacgtcg ccgtggcggc
     4561 cggcgtcgcc acggccccga ccctgctcgc ccggctggcc aaggccttgt tcgaccgagc
     4621 ttacgaggac gacgacagtt ttgtcgccaa ggggcgctcg atgttcgagg cccggcttcc
     4681 acgtctggaa gacccggcca tcagccgcga aaccggcatg ctgctgggc
//
LOCUS       DASWGM010001028         3321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1359213,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001028 DASWGM010000000
VERSION     DASWGM010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359213"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VGA15_27765"
     CDS             <1..994
                     /locus_tag="VGA15_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH--cytochrome reductase"
                     /protein_id="HEX9591528.1"
                     /translation="QIRLQVAEGRRAQLPIGDTVSVGRLLSEFVELQQVATRKQIQIM
                     SEHTRCPVTKPKLLAYVGDDAAASEHYRADILGKRKSVFDLLEEHPACELPFHAYLEM
                     LSLLAPRYYSISSSPSGDPQRCSVTVAVVDAPASSGRGIYKGICSNYLAGRRTGETIQ
                     ATVRETKAGFRLPNEAAVPIIMVGPGTGLAPFRGFLQERAARKAKGAALGPAMLFFGC
                     RHPDQDFLYADELKAFEASGITELHTAFSRAEGPKTYVQNLVAAQQERVWSLIEDGAI
                     IYVCGDGGKMEPDVKAALVAIYRKRNGADADAGLRWIDDLGTSNRYVLDVWAGG"
     gene            complement(1161..2081)
                     /locus_tag="VGA15_27770"
     CDS             complement(1161..2081)
                     /locus_tag="VGA15_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9591529.1"
                     /translation="MNVLAHQPPQIARANGIELCYEIFGEAGAEPMLLIMGLGAQMIH
                     WDDDFCWQLAARGFRVIRFDNRDIGKSSKMTGGKRLTAVELLKLRFLKIPVAAPYKLS
                     DMAEDVIGLMDVLGIKSAHLVGASMGGMIAQELAISFPQRVRSLTSIMSTTGNPKVPP
                     PTREAAAILMAPPPANKEEYYTRFAQTWKILRVGSFPLDESLDRARAERTYARGLSPA
                     GVGRQFRAILASGSRKQRLASVKAPTLVIHGTVDPLVRPEGGKDTAASIPGAKLLMIE
                     GMGHALPIPMWPEIIDAIDKHAHGAAAKAA"
     gene            2262..2648
                     /locus_tag="VGA15_27775"
     CDS             2262..2648
                     /locus_tag="VGA15_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591530.1"
                     /translation="MADNSNDPVAMWQNMIGEMEKGFNAFANQAMASPEFSKVVNQVG
                     GASAGAQKQLGDLMEKYLVGMNLPSRAQMVSMAERLQSIEGQLNEIKALLYQVHNNSG
                     APASGPAATPKPPRTKRPPPPGGEQK"
     gene            2645..>3321
                     /locus_tag="VGA15_27780"
     CDS             2645..>3321
                     /locus_tag="VGA15_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX9591531.1"
                     /translation="MNAAAGLDLQSIPDRIQSEVQRAIQRSIKGVEYIASSGPSLGST
                     PKDILSVRGTMNLYHYRPVSDEIYRVPILIVMATTNRGYILDLVPGQSFIEFLLKRGY
                     DVYMLDWTAPKPEEKSLRMEDYVLDFIPDCVGRVQQDSGETDVTVIGYCFGGVLSLLY
                     GAIFADGPMKNLICFTTPVDFREMKLFQNFSDKRYFDVDRLIDSVGNVPPEMILSSFE
                     MLRPASRA"
BASE COUNT          615 a         1024 c         1062 g          620 t
ORIGIN      
        1 tcagatccgg ctgcaggtcg ccgaaggccg ccgcgcgcaa ttgccgatcg gcgacaccgt
       61 gtcggtcggg cgattgctct ccgaatttgt cgaattgcag caggtcgcga cccgcaagca
      121 gatccagatc atgtcggaac atacgcggtg tccggtgaca aaaccaaaac tgctggccta
      181 tgtcggcgac gatgccgccg catcggagca ttatcgcgcg gatatcctcg gcaagcgcaa
      241 atcggtgttc gatctcctgg aggagcaccc ggcctgcgaa ttgccgttcc atgcctatct
      301 ggaaatgctg tcgctgctgg cgccgcgcta ttattcgatc tcgtcgtcgc cgtcgggcga
      361 tccccagcgc tgcagcgtca ccgtcgccgt ggtcgacgcg cccgcgagtt cgggacgcgg
      421 catctacaag ggcatctgct cgaattacct cgccggccgc cggaccggcg agacgatcca
      481 ggcgacggtg cgcgagacca aggccggttt ccggttgccc aatgaggctg ccgtgccgat
      541 cattatggtc ggccccggaa ccgggcttgc gccattccgc ggcttcctgc aggagcgcgc
      601 cgcccggaaa gccaagggcg cggcgctcgg cccggcgatg ctgtttttcg gctgccgtca
      661 tcccgatcag gattttctct atgccgatga actgaaagcg ttcgaagcca gcggcatcac
      721 cgagctgcat accgcgttct cgcgcgccga gggcccaaaa acctatgtgc agaatctggt
      781 cgcggcgcaa caggagcgcg tctggagcct gatcgaggac ggcgcgatca tctatgtctg
      841 cggcgacggc ggcaagatgg agccggacgt caaggccgcc ctggttgcga tctatcgcaa
      901 gcgcaacggg gccgacgccg atgcggggct gcgctggatc gacgatctcg gcaccagcaa
      961 tcgttacgtg ctcgatgtct gggcgggggg atgaggctgc ctcgacacat attgccgtca
     1021 tcctgaggag cgcgtcttcg cgcgtctcga aggatggcca cgtgcaccgt cgcttgtcat
     1081 ccttcaaggc tcgccgaaga ggctcgcacc tcaggatgac gcccggcgtt tgccgggacg
     1141 accgaaaagt tgggacgacg tcacgccgcc ttggccgcgg cgccgtgggc gtgcttgtcg
     1201 atggcgtcga tgatctccgg ccacatcggg atcggcagcg cgtgacccat gccttcgatc
     1261 atcagaagct ttgcgccagg gatcgaggcc gcggtgtcct tgccgccttc ggggcgcacc
     1321 aggggatcga ccgtgccatg gatcaccagc gtcggcgcct tgaccgatgc cagccgctgc
     1381 ttgcggctgc ctgaggcgag gatggcgcgg aattgccgcc cgacgccggc gggactcagt
     1441 ccgcgcgcat aggtgcgttc ggcgcgggcg cgatcgagcg attcgtcgag cgggaagctg
     1501 ccgacacgca ggatcttcca ggtctgcgcg aagcgcgtgt aatattcctc tttgttggca
     1561 ggcggcggag ccatcagtat ggccgcggcc tcgcgggtcg gcggcggcac ttttggattg
     1621 cccgtcgtcg acatgatgga agtgagcgag cgcacccgct gcggaaacga aatcgccagt
     1681 tcctgggcga tcatcccgcc catcgacgcc cccaccagat gtgccgactt gatgccaagc
     1741 acatccatca ggccgatcac atcctccgcc atgtcggaca gcttgtaggg cgcggccacc
     1801 ggaatcttca ggaaccgcag cttgagcagt tcgacggcgg tcaggcgctt gccgccggtc
     1861 atcttgctgg acttgccgat atcgcgattg tcgaaccgga tgacgcgaaa cccgcgcgcg
     1921 gcgagctgcc agcagaaatc gtcgtcccaa tggatcatct gggcgcccag ccccatgatc
     1981 agcagcatcg gctcggcgcc ggcttcgccg aagatctcgt agcaaagctc gatgccattg
     2041 gcgcgggcga tctgcggcgg ctgatgggcg agtacgttca ccttggattt tccctcaaat
     2101 cggtttttac cgggatttta tggcacggtt tcgtgcactg cagaaggctt gcgaacggca
     2161 gggaggctga caaatcgacc tcgcgacgtt gaccgccgtg gcgcaacagt gtggtatgat
     2221 ttgaaaacag gcgagggact cgtcgtcaat ccggggcgcc aatggcagac aacagcaacg
     2281 atccggtcgc gatgtggcaa aacatgatcg gcgagatgga gaaggggttc aacgccttcg
     2341 ccaatcaggc gatggcgtcg cctgaattca gcaaggtcgt caatcaggtc ggcggggcct
     2401 cggcgggcgc gcaaaagcag ctcggcgatc tcatggagaa atatctcgtc ggcatgaatt
     2461 tgccgagccg ggcccagatg gtcagcatgg ccgaacggct gcagtcgatc gaagggcagc
     2521 tgaacgagat caaggcgctg ctctatcagg tccacaacaa ttccggcgcg ccggcaagcg
     2581 ggcccgctgc tacgccgaag ccaccccgca ccaaacgtcc gccgcctccg ggtggagagc
     2641 aaaaatgaac gccgccgcgg gcctggatct tcaatccatc ccggaccgga ttcaatccga
     2701 ggtgcagcgt gcgatccagc gcagcatcaa gggtgtcgaa tatatcgctt cgtcgggccc
     2761 ttcgctggga tcgacgccaa aggacatcct gagcgtccgc ggcacgatga atctgtatca
     2821 ctaccggccg gtctcggacg agatctaccg cgtgccgatc ctgatcgtga tggcgaccac
     2881 caatcgcggc tacatcctcg atctggtgcc cggccagagt ttcatcgagt ttctgttgaa
     2941 gcgcggctac gacgtctaca tgctggactg gaccgcgccg aagccggaag agaaatcgtt
     3001 gcggatggag gattacgttc tcgacttcat cccggattgc gtcggccgcg tgcagcagga
     3061 ttccggcgag accgatgtca cggtgatcgg ctattgcttc ggcggcgtgc tgtcgctgct
     3121 gtacggcgcg atcttcgccg acggtccgat gaagaatctg atctgcttca ccaccccggt
     3181 tgatttccgc gaaatgaagc tgttccagaa cttttcggac aaacgctatt tcgacgtcga
     3241 tagattgatc gacagcgtcg gcaatgtccc gcccgagatg atcctttcat cgttcgagat
     3301 gttgcggccg gcatcgcgcg c
//
LOCUS       DASWGM010001029         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1235792,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001029 DASWGM010000000
VERSION     DASWGM010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235792"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VGA15_27785"
     CDS             <1..406
                     /locus_tag="VGA15_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9591532.1"
                     /translation="GGSFGRDLAAQGEPAMVAFAVEWLTKLFGSDAAAAVKKSSATRW
                     DVAPFALGAMSVAAPGAQPSRKILTEPIGCMFLAGEATHETLWGTVDGAWESGERAAE
                     AALRKIGALKDTEPAARAAPAVKRRRRGATIN"
     gene            complement(478..1422)
                     /locus_tag="VGA15_27790"
     CDS             complement(478..1422)
                     /locus_tag="VGA15_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX9591533.1"
                     /translation="MIDRRDALKLALCSAAALASPALAQAPAKPRTRIIFLGTKGGPR
                     VTMGASNPANLVMVNDTPYVIDCGAGVSRQLVAAGVPIPSVKYIFISHHHSDHNLEYG
                     NLFYNAWAAGLSTPIHSFGPKGIEAMTQTYWELNRFDVETRIEDEGRPDPRKLLMPKD
                     ITEDGVVLQTADVKVTAFRTPHPPIVDNFAYKFETPDGTIVFSSDTACNPKLAEFAKG
                     ADVLVHECLYVPAVDRLVIKTKNGATLKKHLLEGHTTTEDVGRIAAAAGVKVLVLSHF
                     VPGDDPLVTDDNWTEDVKKNFSGRIIVAKDLMQLTLPV"
BASE COUNT          253 a          442 c          526 g          287 t
ORIGIN      
        1 tggaggctcg ttcgggcgcg atcttgcggc gcagggcgag ccggccatgg tcgccttcgc
       61 ggtggaatgg ttgaccaagc tgttcggcag cgacgcagct gcggccgtca agaaatcgag
      121 cgcgacccgc tgggacgtcg cgccgtttgc gctcggcgcg atgtctgttg cggcgccggg
      181 cgcccaaccg tcgcgaaaaa tcctgacgga gccgatcggc tgcatgttcc tcgcgggcga
      241 ggcgacccat gagacgctgt ggggaacggt tgacggcgcc tgggaaagcg gcgagcgcgc
      301 ggccgaggcg gcgctgcgaa agatcggcgc attgaaggat accgagcccg cagcgcgagc
      361 cgcgccggcg gtcaagcgcc ggcgtcgggg agcgacgatc aattgagaga gccgcgaccg
      421 gttgtcggcg cattgacgac ggtgaccgag acgttcggga tgacggcagc gtcgtcctca
      481 caccggcagc gtcagttgca tcaggtcctt ggcgacgatg atccggccgg aaaaattctt
      541 cttcacgtcc tcggtccagt tgtcatcggt gacgagtgga tcatcgccgg ggacgaaatg
      601 gctgagcacc agcaccttca cgccggcggc ggcggcgatg cggccgacgt cctcggtggt
      661 ggtgtggccc tcgagcagat gtttcttcag cgtcgcgccg ttcttggtct tgatcacgag
      721 gcggtcgacc gcgggcacgt agaggcattc gtgcaccagc acgtcggcgc ctttggcaaa
      781 ctccgccagt tttggattgc aggcggtgtc gctggagaag acgatggtgc cgtcgggcgt
      841 ctcgaatttg taggcgaagt tgtcgacgat gggcggatgt ggcgtgcgga acgccgtcac
      901 ctttacgtcc gccgtctgca gcaccacgcc gtcctcggtg atgtccttgg gcatcagcag
      961 cttgcgcgga tcgggacggc cttcatcctc gatccgggtc tcgacgtcga accggttcag
     1021 ctcccaatag gtttgggtca tcgcctcgat gcccttgggg ccgaacgaat ggatcggcgt
     1081 cgacaggccg gcggcccagg cgttgtagaa cagattgccg tattcgagat tatgatcgga
     1141 atggtgatgg ctgatgaaga tatatttcac cgacgggatc ggcacgccgg cggccaccag
     1201 ttgacggctg acccctgccc cgcagtcgat cacgtacggc gtgtcgttca ccatcacaag
     1261 gttggctgga ttggatgccc ccatggtgac gcgcggcccg cccttggtgc cgaggaatat
     1321 gatacgtgtg cggggttttg caggggcctg cgccagtgcg ggcgaggcca gcgcggctgc
     1381 gctgcaaagg gcgagcttca gcgcatcgcg acggtcgatc atggcgtttc cccaacattt
     1441 cttgttggac gcaacttaac tgtatctgct tttcgcgcaa gcgttcagcg caacacgccg
     1501 tcaagcag
//
LOCUS       DASWGM010001030         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_676802,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001030 DASWGM010000000
VERSION     DASWGM010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676802"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2387)
                     /locus_tag="VGA15_27795"
     CDS             complement(<1..2387)
                     /locus_tag="VGA15_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX9591534.1"
                     /translation="MASTNRLSPIPHPPTKPVVGNMLSLDSTAPVQHLVRLTRELGPI
                     FWLDMMGAPLVIVSGHDLVEELSDEKRFDKAVRGSLRRVRAVGGDGLFTADTSEPNWS
                     KAHNILLQPFGNRAMQSYHPSMVDIADQLVKKWERLNGDEEIDVVHDMTALTLDTIGL
                     CGFDYRFNSFYRRDYHPFVESLVRSLETIMMTRGIPLEGIWLKKRRQELARDVAFMNK
                     MVDEIVAERRKNAEAAEARKDMLGAMMTGVDRATGEQLDDVNIRYQINTFLIAGHETT
                     SGLLSCTLYALLKHPEVRKKAYEEVDRVLGPDTDARPTYQQVTQLTYITQILKEALRL
                     WPPAPAYGIAPLQDETIGGKYKLKKNTFITILVMALHRDPSVWGPNPDAFDPENFSRE
                     AEAARPVNAWKPFGNGQRACIGRGFAMHEAALAIGMILQRFKLIDIHRYQMHLKETLT
                     IKPDAFKIKVRPRSEKDRGAYAGRTTTTVAASTGAPAPRARTRPGHNTPLLVLYGSNL
                     GTAEELATRVADLAEVNGFATKLAALDDFVGKLPEQGGVLIFCASYNGAPPDNATQFV
                     KWLGSDLARDAFSKVRYAVFGCGNSDWLATYQSIPRFIDEQLAAHGASNAYVRGEGDA
                     RDDLDGQFESWFAKLRPLAVKQFGIDTSFDRSADDEPLYKVEPVAPTAVNAILALGGA
                     APMKVLVNTELQNKLGANASDRSTRHIEVQLPSGVTYRVGDHLSVVPRNDPVLVDSVA
                     RRFGFLPADQIRLQVAEGRRAQLPIGDTVSVGRLLSEFVELQQVATRKQIQIMSE"
     gene            complement(2550..3545)
                     /locus_tag="VGA15_27800"
     CDS             complement(2550..3545)
                     /locus_tag="VGA15_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9591535.1"
                     /translation="MIEMPPLQFAETNGIRMGYYEAGPKTDAPPVILCHGWPEIAFSW
                     RHQIKALGDAGIRVIAPDQRGYGATDRPEPVEAYDIEHLTDDLVGLLDHLRIDKAIFV
                     GHDWGGFIVWQMPLRHIDRVAGVVGINTPHTNRAPADPIELLRRRFGEHMYIVQFQDP
                     AREPDRIFGGRVEQTFDAFMRKPAPRGDTAPEEQVVAGIGASPRLNLAFPQMIAGYDA
                     KHDPRTPILSPEEKKVFVDTFTRTGFTGGINWYRNMSRNWLRSAQIDHTVRVPSLMIM
                     AENDSVLPPSAADGMEKIIPDLEKYLVRGSGHWTQQEKPEEVSAKLIEWRKRRFG"
     gene            complement(3594..>4077)
                     /locus_tag="VGA15_27805"
     CDS             complement(3594..>4077)
                     /locus_tag="VGA15_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HEX9591536.1"
                     /translation="CSIATNIEDPKARLEAIIAQSTKSKEMSHPLRALMPQVSNLSML
                     GAPILVQVLALLYSRSNLSDVLPPAANITVSNVPGPRQTLYAAGAELLHIFPVSISTH
                     GLALNITVQSYRDQLDFGFIAGANIIPHVQVLCDMLPAEFAALEAAFAPPADLKSAAG
                     "
BASE COUNT          719 a         1270 c         1300 g          788 t
ORIGIN      
        1 tgttccgaca tgatctggat ctgcttgcgg gtcgcgacct gctgcaattc gacaaattcg
       61 gagagcaatc gcccgaccga cacggtgtcg ccgatcggca attgcgcgcg gcggccttcg
      121 gcgacctgca gccggatctg atccgcaggc agaaacccga aacgccgcgc caccgaatcc
      181 accagcacgg gatcgttgcg cggcaccacg ctgagatgat cgccgacccg gtaggtgacg
      241 ccggaaggca attgcacctc gatgtgccgg gtcgatcgat ccgaggcatt ggcgccgagc
      301 ttgttttgca gttcggtgtt gaccagcacc ttcatcggcg cggcgccgcc gagggccaag
      361 atcgcattca ccgccgtcgg cgccaccggc tcgaccttgt acagcggctc gtcgtcggcg
      421 ctgcgatcga aactggtatc gatgccgaat tgcttcaccg ccagcggacg cagtttcgcg
      481 aaccaggatt cgaactggcc gtcgagatcg tcgcgggcgt cgccctcgcc gcgcacatag
      541 gcgttgctgg cgccgtgcgc cgccagttgt tcgtcgatga agcgggggat cgactgatag
      601 gtcgcaagcc agtcgctgtt gccgcagccg aacaccgcgt agcgcacctt tgagaatgca
      661 tccctggcta agtcgctgcc gagccatttg acgaactgcg tggcattgtc tggcggggcg
      721 ccattgtacg aggcgcagaa aatcaggacg ccgccctgct ccggcaattt gccgacgaaa
      781 tcatcgaggg cagcgagttt ggtcgcaaag ccgttgacct cggccaaatc tgccacgcgt
      841 gtcgccagtt cctcggcagt gccgagattc gatccgtaca gcaccaggag cggcgtattg
      901 tgccccgggc gggtccgggc gcgcggcgcc ggcgcgccag tcgatgcagc caccgttgtc
      961 gtggtgcggc cggcataggc gccgcgatcc ttttcgctac gcgggcgcac cttgatcttg
     1021 aaggcgtccg gcttgatggt cagcgtctcc ttcaggtgca tctggtagcg atggatgtcg
     1081 atcagcttga accgctgcag gatcatgccg atcgccagcg ccgcttcatg catggcaaag
     1141 ccgcggccga tgcaggcgcg ctggccgttg ccgaacggct tccaggcatt gacgggacgc
     1201 gccgcctcgg cctccctgct gaaattctcc gggtcgaacg cgtcgggatt gggaccccag
     1261 acgctggggt cacgatgcag cgccatcacc aatatcgtga tgaaggtatt cttcttgagc
     1321 ttgtacttgc cgccgatggt ttcgtcctgg agcggcgcaa tgccataggc cggcgcgggc
     1381 ggccaaagcc gcaaggcctc tttgagaatc tgcgtgatgt aggtgagctg cgtcacctgc
     1441 tggtaggtcg gcctcgcatc ggtatcgggg ccgagcaccc ggtcgacctc ctcataggct
     1501 ttcttgcgca cctcggggtg ttttagcagc gcgtacagcg tacaggacaa caggccgctg
     1561 gtggtctcgt gacccgcgat caggaaagtg ttgatctggt agcggatgtt gacgtcgtcg
     1621 agctgctcgc cggttgcgcg gtcgacgccg gtcatcatgg cgccgagcat gtccttcctg
     1681 gcctcggcgg cctccgcatt cttgcggcgt tcggcgacga tctcgtcgac catcttgttc
     1741 atgaaggcga cgtcgcgcgc aagctcctgc cggcgctttt tcagccaaat gccctcgagc
     1801 ggaatcccgc gggtcatcat gatggtttcg agcgagcgca ccagcgattc cacgaacgga
     1861 tgataatcgc gccggtagaa tgaattgaat cgatagtcga aaccgcacag cccgatggtg
     1921 tcgagcgtca gcgccgtcat gtcgtgaacg acgtcgatct cctcgtcgcc gttcagccgc
     1981 tcccatttct tcaccagctg atcggcgata tcgaccatgc tcgggtgata ggactgcatg
     2041 gcgcgattgc cgaacggctg cagcaggata ttgtgcgcct tgctccagtt cggctctgac
     2101 gtatcggccg tgaacagtcc gtcgccgcca accgcgcgga cgcggcgcaa cgagccacgc
     2161 accgccttgt cgaaacgctt ttcgtcgctg agttcctcga cgagatcgtg gccggaaacg
     2221 atgaccagcg gtgcgcccat catatcgagc cagaagatcg gtccgagctc cctggtcaac
     2281 cgcaccagat gctgcaccgg cgcggtcgaa tccagcgaca gcatgttgcc gaccaccggt
     2341 ttcgtcggcg gatgcgggat cgggctcagt cggttggtgg acgccatcga ggtagtgtct
     2401 cctgctgggt gtatcgtgcc ccggacgcgg cgcagcgcgc tagcgatgcg ccgctgagcc
     2461 ggggcccacg ctacgagcac cgttgcattg tgggtcccgc tctgcggagc agcgtgaagg
     2521 acgctgcatc gcgtcgggga ccgatgcccc tatccaaatc ttcgcttacg ccattcgatc
     2581 agcttggcgc tgacctcttc cggcttttcc tgctgcgtcc aatggccgct gccccgaacc
     2641 aggtactttt ccagatcggg aatgattttc tccatgccgt cggccgccga cggcggcagc
     2701 acggaatcgt tctcggccat gatcatcagc gacggcaccc gcacagtgtg atcgatctgc
     2761 gccgaacgca gccagttgcg cgacatgttg cggtaccagt tgatgccgcc ggtgaagccg
     2821 gttctggtga aggtatcgac aaacaccttc ttttcctccg gcgacaggat cggcgtgcgc
     2881 ggatcatgtt tggcgtcgta gccggcaatc atttgcggaa acgccagatt taaccgtggc
     2941 gaagcgccga tgccggcgac gacctgctcc tccggcgcgg tgtcgccgcg cggcgccggc
     3001 ttcctcataa aggcgtcaaa ggtctgttcg acccggccgc caaaaatcct gtcgggctcg
     3061 cgcgcaggat cctgaaactg cacgatatac atgtgctcgc cgaaacgccg gcgtaacagt
     3121 tcgatcggat ccgccggcgc gcggttggtg tgcggcgtat tgatcccgac cacgccggcg
     3181 acgcggtcga tgtgccgcag cggcatttgc cagacgataa aaccgcccca gtcatgaccg
     3241 acgaaaatcg ccttgtcgat cctgaggtgg tcgagcagcc cgacgagatc gtcggtcagg
     3301 tgctcgatat cataggcttc gacgggttcg ggccggtcgg tcgccccata gccgcgctga
     3361 tccggcgcga tcacccgaat tccggcatcg cccaacgcct tgatctggtg gcgccacgaa
     3421 aacgcgatct ccggccagcc gtggcagaga ataaccggcg gcgcatcggt tttcgggccc
     3481 gcttcgtaat agcccatgcg aatgccgttg gtctcggcga actgcagcgg cggcatctcg
     3541 atcatcggca atgtctcaca tttatcctgg gcatgatctg atcggatcgt gcgctagcct
     3601 gccgcgctct tgagatcggc cggcggcgcg aaggcggctt cgagggcggc gaattccgcc
     3661 ggcagcatgt cgcacaaaac ctgcacatgg ggaatgatgt tggcgccggc gataaaacca
     3721 aaatccagtt gatcgcggta gctctgcacg gtgatgttga gcgcaaggcc gtgggtcgag
     3781 atcgacaccg gaaagatgtg cagcaattcc gcccccgccg catacagcgt ctgacgcggc
     3841 ccgggaacgt tggagacggt gatgttcgcg gccggcggca agacgtccga caggttggag
     3901 cggctataca gcaacgccag aacctgaacc aggatgggcg cgccgagcat cgagaggttg
     3961 gagacctgcg gcatcagggc gcgcagcgga tgcgacattt ccttcgactt ggtggattgc
     4021 gcgatgatcg cttccagccg cgccttggga tcctcgatgt tggtggcgat cgagcag
//
LOCUS       DASWGM010001031         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1501930,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001031 DASWGM010000000
VERSION     DASWGM010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501930"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VGA15_27810"
     CDS             <1..776
                     /locus_tag="VGA15_27810"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9591537.1"
                     /translation="DAAKKYPKVEFRHPTSLWQADKHPANAGGYFCYLDQGHYVNGIA
                     AGLSTKSNKIGYIAAKPIPLVLRNVNSFMLGARKVNPNATVQMIVTGDWALPVREAEA
                     ANALVDAGCDIIACHIDSPKVVIETAEKRGVKSCGHNANQADLAPKGFITGAELKWGT
                     IYKSFAGMIAKGEKLPNMVIGGYDKEMVASSPFGAGASEAGRNAAMAAIADLKAGKPI
                     FVGEVKSNTGKVITSKDTALYDPSLETTDYLVEGIVGSV"
     gene            1118..1843
                     /locus_tag="VGA15_27815"
     CDS             1118..1843
                     /locus_tag="VGA15_27815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX9591538.1"
                     /translation="MANSKYTSRGTLAAEPEPIDLDWSTTALLIIDMQRDFMEPGGFG
                     ETLGNDVSQLARAVKPIAAVLAAAREQGMLVVHTREGHLPDLSDAPPAKVERGAPSLR
                     IGDPGPMGRILIRGEAGHDIIPALYPLDSEVVIDKPGKGAFYATELGDTLQKYGIENL
                     LVCGVTTEVCVNTTVREANDRGYRCVVLADGCASYFPEFHEMGLRMIKAQGGIFGWVS
                     DSAAVLKAMTPETSNNLAAGASR"
     gene            1840..>2589
                     /locus_tag="VGA15_27820"
     CDS             1840..>2589
                     /locus_tag="VGA15_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HEX9591539.1"
                     /translation="MSTSTTGTAAKSDFKPVLWTPGDWNAFFGFGTNILVNMLVLTGL
                     LRFVLKMPDSLVFGRILPALGLMMCLSTFYYAWLAYRLAQKTGRADVCALPSGVSVPH
                     MFIVTFVIMLPITIKTGDPLKGWSAGLVWVFFQSFILMIGGFIAPYIRKITPRAALLG
                     TLAGVSVTFISMRPALEMYMTPQIGLVCFAIILVSWFGGVKYPKGIPAGLVAIAVGMI
                     IAWGSNLFGLGLGGLSIKGVGDAFASFGFSVP"
BASE COUNT          503 a          796 c          796 g          494 t
ORIGIN      
        1 tcgatgccgc gaagaaatat cccaaggtcg aattccgcca tccgacgtcg ttgtggcagg
       61 ccgacaagca tccggccaat gccgggggtt atttctgcta tctggatcag ggacactacg
      121 tcaacggcat cgccgccggt ctgtcgacca agtcgaacaa gatcggctac atcgcggcca
      181 agccgatccc gctggtgttg cgaaacgtca actcgttcat gctcggcgcg cgcaaggtca
      241 accccaatgc caccgtgcaa atgatcgtca ccggcgactg ggcgttgccg gtgcgcgagg
      301 ccgaggccgc caatgcgctg gtcgacgccg gctgcgacat cattgcctgc cacatcgaca
      361 gccccaaggt ggtgatcgag acggcggaga agcgcggtgt gaagagttgt ggccacaatg
      421 ccaaccaggc cgacctcgca ccgaaggggt tcatcaccgg cgccgagttg aagtggggaa
      481 cgatctacaa gagctttgcc ggcatgatcg ccaagggcga gaagctgccc aatatggtga
      541 tcggcggtta cgacaaggag atggtggcga gttcgccctt tggcgccggc gccagcgagg
      601 ccggcaggaa tgccgccatg gccgcgatcg ccgatctcaa ggccggcaag ccgatctttg
      661 tcggcgaggt caagagcaac accggcaaag tcattaccag caaagacacc gccctctatg
      721 atccgtcgct cgaaaccacc gattacctgg tcgagggaat cgtcggtagc gtctgattcc
      781 aaggtaactt cggcagcaaa tagtgtgtcg cggtcgcctt gcggatcgtc ccaagcctcg
      841 tgatcgtcgc ggcgcggcat gccgttacgg ccgcgcttct cttgcgaagt tccgcgtcac
      901 tctgcgagat gccgcatcaa tcatcacgcc caatgaataa gcaagtgcac gtcgcaaaag
      961 cgacgcttag ggaataggca gagctcaatg caaaatacaa ctaactgaat ttacaaaaga
     1021 ttccaaaatc tggcggcgcc taaaggcgtt gtacacaatg gcatgcgcct tgcttagtta
     1081 gctccgagag ttctctcgct caaggagcgc gtgcgtcatg gcgaactcaa agtatacatc
     1141 ccgcggcacc ctggctgcgg agccagagcc gatcgatctc gactggtcga ccaccgcact
     1201 cctcatcatc gacatgcagc gcgatttcat ggagcccggc ggcttcggcg agacgctcgg
     1261 caacgatgtc tcgcaactcg cccgtgcggt gaagccgatc gcggcggtgc tggcggcggc
     1321 gcgcgagcag ggcatgctcg tggtgcacac ccgcgaaggt catctgcccg atctgtccga
     1381 tgcgccgccg gcgaaggtcg aacgcggcgc gccaagcctg cgcatcggcg atcccggccc
     1441 gatgggacgg atcctgatcc gcggcgaagc cggccacgat atcattcccg cgctgtatcc
     1501 gctggacagc gaagtcgtga tcgacaagcc cggcaagggc gcgttctacg cgaccgaact
     1561 cggcgatacc ttgcagaaat acggcatcga aaatcttctg gtgtgcggcg tcaccaccga
     1621 agtctgtgtc aacaccacgg tgcgcgaggc caacgaccgc ggctatcgct gcgtcgtgct
     1681 ggccgacggc tgcgcctcct atttccccga attccacgag atgggcctga ggatgatcaa
     1741 ggcccagggt ggcatcttcg gctgggtcag cgattcagcc gcggttctca aggcaatgac
     1801 accggagact tcaaacaatc tggcagcggg agcatcacga tgagcacgag cacgacgggg
     1861 actgcagcaa aatccgattt caagccggtg ttgtggacgc cgggcgactg gaacgcgttc
     1921 ttcggtttcg gcaccaacat cctcgtcaac atgctggtgc tgacggggct gttgcgcttc
     1981 gtgctgaaga tgcccgactc gctggtgttc ggccgcatcc tgccggcgct cggcctgatg
     2041 atgtgcctct cgacatttta ctatgcgtgg ctggcctatc ggctggcgca gaagaccggc
     2101 cgtgccgatg tctgcgcgct accctccggc gtcagcgtgc cgcacatgtt catcgtcacc
     2161 ttcgtgatca tgctgccgat caccatcaag accggcgatc ccctgaaggg ctggtccgcg
     2221 ggcctagtct gggtgttctt ccagagtttc attctgatga tcggcggctt catcgcgcca
     2281 tacattcgga aaattacgcc ccgcgcggcg ttgctcggca cgctggccgg cgtctccgtg
     2341 accttcatct cgatgcggcc ggcattggag atgtacatga cgccgcagat cggcctcgtc
     2401 tgcttcgcca tcatcctggt gagctggttc ggcggcgtga aatatccgaa gggaattcca
     2461 gccgggctcg tcgccatcgc ggtcggcatg atcatcgcgt ggggctcgaa cctgttcggg
     2521 cttgggctcg gtggcctcag catcaagggc gtcggcgacg cgtttgcgag tttcggcttc
     2581 tcggtgccg
//
LOCUS       DASWGM010001032         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_678082,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001032 DASWGM010000000
VERSION     DASWGM010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678082"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..595
                     /locus_tag="VGA15_27825"
     CDS             128..595
                     /locus_tag="VGA15_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591540.1"
                     /translation="MPLLSEHRDPRELSDRLRDAHAATADLVAEIIGETCRRFPSTGQ
                     SEKTARIERLIQTDAWTDAALALIDLELPQWQVRRIAYDEGEWYCALSRERELPDWLD
                     QSIEAHHPDLPLAILSAFVEAQRVAAPSSRTSVPTVPRAASTFYESVLTDNFA"
     gene            761..976
                     /locus_tag="VGA15_27830"
     CDS             761..976
                     /locus_tag="VGA15_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591541.1"
                     /translation="MFILALFATFGGIGFGRSLAALLCMSTILSAVIGAMRREPPFDI
                     VLNHWDETVAYAALCCLASGLNQAAPF"
     gene            1290..2093
                     /locus_tag="VGA15_27835"
     CDS             1290..2093
                     /locus_tag="VGA15_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX9591542.1"
                     /translation="MNIISADLSTILDRILDTAADNFRIAKILVQLGLDPNNITYDAL
                     FNRLLEIFLANITLANMFALVGAIFFVATLLTQTMVPLRVANMVGCAFIVGYGALAGD
                     VKTFLLYLLLLPINAFRLRQMLNLVKKARSATQGDTSMEWLKPFMTERKYRKGDVLFK
                     KDDAANEMFLTITGKFLVTEIGIEIPPGRLMGELGFLSPNNRRTATVECTEDGLVLTI
                     TYEKLLEIYFQNPQFGYYFLVLTSQRLLENISRLEAIIAQNKITQHTPP"
     gene            complement(2119..>3375)
                     /locus_tag="VGA15_27840"
     CDS             complement(2119..>3375)
                     /locus_tag="VGA15_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9591543.1"
                     /translation="PAETIREVARMYARSRASIIFWGMGISQHVHGTDNARCLIALAL
                     ITGQVGRPGTGLHPLRGQNNVQGASDAGLIPMFLPDYQPVGRTDLREPFEKLWHQDLD
                     PVRGLTVVEIINAIHAGEITGMYIEGENPAMSDPDLQHAREALAKLDHLVVQDLFVTE
                     TAFHADVILPASAFAEKSGTFTNTDRRVQLAREVIKPPGDARQDLWIIQEIARRMGLD
                     WRYDGPADVFAEMTEVMPSLKNITWERLVREGAVTYPVDDPHQPGNEIIFTTGFPTES
                     GRGKIVPAHVLPPDELPDDEYPMVLSTGRVLEHWHTGSMTRRSLVLDQIEPEAVAFMS
                     PKDMRRLKVWPGDFIRLETRRGAVEIKVRSDRDVPENMVFMPFCYAEAAANLLTNPAL
                     DPFGKIPEFKFCAVRAERAEIRSAAE"
BASE COUNT          623 a         1062 c         1002 g          688 t
ORIGIN      
        1 ttccccgccg gcacttatga aatccctata tcttggggcc ctgccccatc tcgttttcaa
       61 atggcgcttt atccatcttg cccggtgcgg ctgcagatcg cggccgagcc ttatgtgaga
      121 tgacgtcatg ccgctccttt cagaacaccg agacccccgc gaactcagtg atcggcttcg
      181 cgacgcccat gcggcaacgg ctgatctcgt agcggagatt atcggcgaga cctgccggcg
      241 ctttccctcg acgggacagt ccgagaagac ggcgcgaatc gaacgattga tccagaccga
      301 tgcctggacc gatgcagcgc tggcgctgat cgatttggaa ctgccgcaat ggcaggttcg
      361 ccgcatcgcc tatgacgagg gcgagtggta ttgcgcgctg tcgcgcgagc gcgagcttcc
      421 ggattggctc gatcaatcga tcgaggccca tcatccggat ttaccgctgg cgattttgag
      481 cgcgttcgta gaagcccagc gcgttgccgc gccgtcgagc aggaccagcg tcccgacggt
      541 gccgcgcgcc gcgagcacgt tttacgagtc ggtgctcacc gacaacttcg cgtaaagccg
      601 cgaacgaact gatgtcgcag cttgctgcgt ctccatgcct tcgatcgtgt cccgggggcc
      661 gcgcaaggcg catccgtcag ggttttgaaa cttggcacaa atcccgaatc tgccgcgaca
      721 actgagatcg atccaggtga tcgctcgctt ttgcctgcga atgttcattc tggccctttt
      781 tgcaacgttt ggcggcatcg gcttcggcag aagccttgcc gcgctattgt gcatgtcgac
      841 catcctcagc gccgtcatcg gcgccatgag gcgcgagcct ccgttcgaca tagttctcaa
      901 tcactgggat gagacggtgg cctatgcggc tctctgctgc ctggccagcg gactgaacca
      961 agctgctcca ttttgaagcg gtgggcgacc ttatacggcg cactcccgtt gcgatgccac
     1021 agctggtcgg cttggcaatc gccgcagcgg ccggcgagcg ccatcggcag cagcgatgca
     1081 tgacggcgtc tttgcccggg cctcggcggg tagcgctgtg ctggcgccgg tcgcccatgc
     1141 cataatcgct gtttcgctgg aagcaggcgg ctcgcgggtg gtggctccga cagccgcggg
     1201 tttgccgttg gcgccgtatc cggcgttgac cgggtctttt gtttgagtca cagaaacatt
     1261 ttgcttcgcc gagacgcgcg ggagattcga tgaacatcat cagtgctgac ctgtcgacga
     1321 tcctggaccg cattctcgac acagcggcgg acaatttcag gatcgcgaag atcctggttc
     1381 aactgggtct cgaccccaac aacatcacat acgacgcatt attcaaccgc ctgctggaaa
     1441 tctttctggc caacatcacc cttgccaaca tgtttgccct ggttggcgcc atcttctttg
     1501 tcgccacctt gctgacgcag accatggtgc cgctgcgcgt cgcgaacatg gtcggctgtg
     1561 cgttcatcgt cggctatggt gcgctggccg gagacgtcaa gactttcctc ttgtacctgc
     1621 tgttgctccc catcaacgcc tttcgccttc gtcaaatgct caacctcgtc aagaaggcgc
     1681 gtagcgcaac gcagggcgat acgtcgatgg aatggctcaa gccgttcatg accgagcgca
     1741 aatatcgcaa gggcgatgtt ctgttcaaaa aggacgatgc agccaacgaa atgttcctga
     1801 ccatcaccgg aaaattcctg gtcaccgaaa tcggcatcga aattccgccg ggacgcctga
     1861 tgggcgaact cggcttccta tcgcccaata accggcggac ggcgacggtc gaatgcacgg
     1921 aggacggcct cgtgctgacc ataacctatg aaaagctgct ggagatctac ttccagaatc
     1981 cgcaattcgg ctattatttt ctcgttctga ccagccagcg cctgctggag aatatctcga
     2041 gactagaggc gattatcgcg cagaacaaaa tcactcagca cacgccgccg taggcggccc
     2101 gaactgagcg cttcgcatct actccgccgc gctgcggatc tcggcgcgtt cggcgcgcac
     2161 ggcgcagaac ttgaattcgg gaatttttcc aaatggatcg agcgccggat tggtcaacag
     2221 gtttgccgcc gcctccgcgt agcagaacgg catgaacacc atgttctcag ggacatcgcg
     2281 atcggagcgc accttgattt cgaccgcgcc gcgccgcgtc tccagccgga tgaaatcgcc
     2341 aggccacact ttcaagcgcc gcatgtcctt tggcgacatg aatgccaccg cctccggttc
     2401 gatctgatcg agcaccaggg aacgccgtgt catcgagccg gtgtgccagt gttcgagcac
     2461 gcgccccgtg gagagcacca tcggatattc gtcgtcgggc aattcgtccg gcggcaacac
     2521 atgcgccggc acgatcttgc cgcggccact ctcggtcgga aaaccggtgg tgaagatgat
     2581 ctcgttaccg ggctggtggg gatcgtcgac cggataggtc accgcgccct cgcgcaccag
     2641 tcgctcccat gtgatgttct tcagcgacgg catcacctcc gtcatctctg cgaagacatc
     2701 agccggcccg tcatagcgcc agtcgagccc catccgcctg gcgatctcct ggatgatcca
     2761 cagatcctgc cgcgcgtcgc ccggcggttt tatgacttcg cgcgcgagct gcacgcggcg
     2821 gtcggtattg gtgaaggtgc cggacttttc cgcgaacgca gacgccggca ggatcacgtc
     2881 ggcatgaaac gcggtctcgg tgacgaacag gtcctgcacc acgagatgat cgagttttgc
     2941 cagtgcctcg cgcgcatgct gcaggtcagg gtcggacatc gcggggttct cgccctcgat
     3001 atacatgccg gtgatctcgc cggcatggat cgcgttgatg atctcgacca cggtgaggcc
     3061 gcgaaccgga tcgaggtcct gatgccaaag cttctcgaac ggttcgcgca gatcggttcg
     3121 cccgaccggc tgataatccg gcaggaacat cggaatcagg ccggcgtcgg aggcgccctg
     3181 cacgttgttc tggccgcgca gcggatgcag cccggtgccg ggacggccga cctgcccggt
     3241 gatcagcgcc agcgcgatca ggcagcgcgc attgtcggtg ccgtgcacat gctggctgat
     3301 gcccatgccc cagaagatga tcgaggcgcg cgaacgcgca tacattcgcg ccacttcgcg
     3361 gatcgtctcc gccgg
//
LOCUS       DASWGM010001033         2495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1937657,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001033 DASWGM010000000
VERSION     DASWGM010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937657"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VGA15_27845"
     CDS             <1..376
                     /locus_tag="VGA15_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9591544.1"
                     /translation="KAIELLARFGLADLADRPISELAGGVRKLVDIAMALVRRPKLLL
                     LDEPTSGVSAEEKFATMDRVIHAVAPDAATIVFVEHDMEIVSHYADRVVAFYQGRILA
                     DGEPREVLTDPEVRRYVTGGAR"
     gene            373..1038
                     /locus_tag="VGA15_27850"
     CDS             373..1038
                     /locus_tag="VGA15_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9591545.1"
                     /translation="MSAVAPMLEIDHLVVEIQSMPALRGFSMHVARGAIASLVGRNGA
                     GKTTLMRSIMGHLKPAQGAIRFEGADLSARPRHARAALGIGYMPEDRGLVPQLTVEEN
                     ILLPLWVADHLDRKARLDFVYEVIGELTEMRHRKALLLSGGQQKLVALGRALGIGTKC
                     LLLDEPFEGIAPALSERLSEVLASLKGKDLTFLMSQSDMNHSRGLIDVEFTIERGANL
                     ASE"
     gene            complement(1063..1305)
                     /locus_tag="VGA15_27855"
     CDS             complement(1063..1305)
                     /locus_tag="VGA15_27855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591546.1"
                     /translation="MHGSFDRSGKSNNRRGWGIGFIALPVLLVIALIGLSMIQPAAPA
                     WISEAVQAEFVGTDLAPGTRPTQLAQPSMAGTVKAY"
     gene            complement(1518..2252)
                     /locus_tag="VGA15_27860"
     CDS             complement(1518..2252)
                     /locus_tag="VGA15_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX9591547.1"
                     /translation="MPQDNTGEARGFPSSKLAILRKHPIFCDLDNEAFEQLCRYAKHS
                     TLKRGTTIVSKGDPGNSLIAVISGTVKISVSSPDGRSAIFNLIGPGEIIGEIAVLDGQ
                     ARTADATANTNCEIFVIDRREFLPFVRSHPALAMKFIELLCAKLRWTSDQVEQVILQN
                     LPGRLASALIRLAEKHEDAPGGRTIAVTQQEISEMVGMTRESINKQLRVWATRHWVRL
                     EHGAIVVLDPESLQALAEAGSGNEGD"
BASE COUNT          492 a          754 c          755 g          494 t
ORIGIN      
        1 caaggcgatc gaattgctgg cgcggtttgg gcttgccgat ctcgccgacc gcccgatctc
       61 tgagctcgcc ggcggcgtgc gcaagctggt cgacatcgcc atggcgctgg tccgccggcc
      121 gaaattgctg ctgctggacg aaccgacatc aggcgtttca gcggaagaga aattcgctac
      181 gatggatcgc gtgatccatg ccgttgcgcc cgatgccgca accattgtct tcgtcgaaca
      241 cgacatggag atcgtcagcc actatgccga ccgggtggtg gcgttttatc agggccgcat
      301 cctcgccgat ggcgaaccgc gcgaagtcct gacggatccg gaagtgcgcc gctacgtcac
      361 cgggggcgcg cgatgagcgc ggtagcgcca atgcttgaaa tcgaccacct cgtcgtcgag
      421 atccagtcga tgccggcgct gcgcggcttt tcgatgcatg tggcaagagg cgccatagcg
      481 agccttgtcg gccgcaacgg cgccggcaag accacgctga tgcgatcgat catgggtcat
      541 ctcaaaccgg cgcaaggcgc cattcggttc gaaggggccg atctgtccgc aaggccgcgc
      601 catgcccgcg cggcgcttgg gatcggctac atgcccgaag atcgcggcct ggtgcctcag
      661 ctcacggtag aggaaaacat cctgctgccg ctgtgggtcg cagaccatct cgaccgcaag
      721 gcgcggctcg acttcgtcta cgaggtgatc ggcgaactca cggaaatgcg ccatcgcaag
      781 gcgctgctgc tgagcggagg ccagcagaag ctggtagcgc ttggccgcgc gctcggtatc
      841 ggcacaaaat gcctgctgct cgatgagccg ttcgagggaa tagcgccggc gctttccgag
      901 cggctgtccg aggtcctggc ctcgctcaag ggcaaggatc tcaccttcct gatgtcgcaa
      961 tcggacatga atcattcgcg cgggctgatc gatgtcgagt tcacgatcga acgcggcgcc
     1021 aacctcgcat ccgaataagc cgactaactg cgcgctctac cgtcaatagg ctttgacggt
     1081 cccggccata ctcggctgcg caagctgcgt cggccttgta cccggcgcca aatcggtgcc
     1141 gacaaactca gcctgcacag cttccgatat ccaggcgggc gcggccggtt ggatcatcga
     1201 caatccgata agcgcgatca cgagaagcac gggtaacgca ataaatccaa tgccccagcc
     1261 ccgacggttg ttgctcttgc ctgatcggtc gaacgagccg tgcatggatg gatgccccca
     1321 ttgtgcggat ttgcagccac gtgactagct caatgcgtgc cgggcccatg tgaagcgcct
     1381 cacatatgcc gagataaaac agttgggaaa tgggtcgcca accgtcggag cggttgagag
     1441 gcgcacggcg tccagcgggc aaccgtgcgc cagaagtctg atccagcttg actgaatcag
     1501 accttcgggg ctgggctcta atcgccttcg tttcccgatc ccgcttcggc tagcgcctga
     1561 agcgattcgg gatccaacac aacgatggcc ccatgttcga gacgaaccca gtggcgggtg
     1621 gcccaaaccc gcaactgctt gttgatgctt tccctggtca tgccgaccat ctcgctgatc
     1681 tcctgctggg tgacggcgat cgttcggccg ccaggcgcgt cctcgtgttt ttcggccagc
     1741 cgaataagcg cgctggcgag ccggccgggc aggttctgaa ggatgacctg ttcgacctgg
     1801 tcactggtcc accgcagctt cgcgcaaagc agctcgatga atttcatcgc gagcgccgga
     1861 tggcttcgta caaaaggaag aaattcgcgg cgatcgatca caaagatctc gcaattcgta
     1921 tttgccgtgg cgtcggctgt ccgcgcttgc ccatcgagca ccgcgatctc tccgatgatc
     1981 tcgccggggc cgatcagatt aaaaatcgcg ctgcggccgt cgggagagga gacgctgatc
     2041 ttcacggtgc cgctgatcac ggcgatgagg ctgttgccgg gatctccctt ggagacgatc
     2101 gtggttcccc gcttcagcgt ggagtgcttg gcgtaacggc acagctgttc gaaggcctca
     2161 ttatcaagat cgcaaaagat cggatgcttg cgcaggatcg caagtttgct gctcggaaac
     2221 ccgcgagctt ctcctgtatt gtcctgaggc acgccggtca cgaccattta tatgcgtttt
     2281 ccatcatcga cggctcgcgc ccaatgctgg gtccgacgag agccatcaat ccgccgcgct
     2341 ttccgaatta tacgccccga tggtgcgcac agcaagaaaa ggcctgaatt tccttcactc
     2401 atttgctcgt aaagggtgag aataattgga tagcaatttt agattatcat acgggaaacg
     2461 cgtggcagat tgatggtgca aacgccgact atcaa
//
LOCUS       DASWGM010001034         3677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_259442,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001034 DASWGM010000000
VERSION     DASWGM010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259442"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VGA15_27865"
     CDS             <1..355
                     /locus_tag="VGA15_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9591548.1"
                     /translation="AGQIHGGVVQGIAQALYEEVSYDPDNGQLLTGSLLDYAVPRAEH
                     VPNIRSFFQETPSPTNPIGVKGIGESGSIAAPPCMVHAVLDALSPFGISHLDMPLTPP
                     RIWSAIQSARAGASQ"
     gene            352..1215
                     /locus_tag="VGA15_27870"
     CDS             352..1215
                     /locus_tag="VGA15_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9591549.1"
                     /translation="MIPASFDYVRATSLSQAIDLLQNDPDGTRLVAGGHTLIPTLKLR
                     LASPSLLIDIGSIDELRGIETGGGRIRIGALTTHAELLASGKLREVLPIFGQAANLIA
                     DPQVRNRGTIGGSLANADPAADWPAVVIALKAELEIAGPKGRRRVAATDFFVDIFTTA
                     LEAGEVLAAIHIARPEPGTRFTYRKIRHPASGYAVVGVAAGLRLKDGVVTEISIGVTG
                     AAGRAFAADAATDFLSGKTPSRENIETAAALVSENTDCLSDRYASAEYRANLVKTEIA
                     RALASLLALSP"
     gene            complement(1195..1749)
                     /locus_tag="VGA15_27875"
     CDS             complement(1195..1749)
                     /locus_tag="VGA15_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX9591550.1"
                     /translation="MSDAKRSRIANKKCSMARAMEVVGDRWSILILREAYYGVKRFDE
                     FEYYVGVAPNILSSRLKKFVDAGVMTRIPLPEHSGRYEYVLTEKGRDFFPAYLALKKW
                     GDDWLAEPEGPQVVFRDRAAGREIKYPKLLTASGKPLRLEDVEVVAGAGAVAFNRKRF
                     GGAADAGPAKAGTKSRVLSRRKRK"
     gene            complement(1861..3126)
                     /locus_tag="VGA15_27880"
     CDS             complement(1861..3126)
                     /locus_tag="VGA15_27880"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX9591551.1"
                     /translation="MSGPLNGIRVVDFSRVLAGPLCARTLQDLGAEVIKVEPPSPDVS
                     RFAFPSTDGMSGYYAQQNAGKRNVSINLNIPGAYELALKLCDTADIVVENFRAGTLGF
                     FGLDYETLSKRNPRLIYASITGYGQGGPWRSRMAYAPAVQAEAGFTENSVRHYGSALT
                     EPRTDSLSHADVYAGLQAVIAILAALNSRQKTGLGQYIDVAMAATLLAVNERAHVDLS
                     DDDIGAEPAVLGATDCSFFTGPQGEQFTVATSIIGSRTFPSWLRAMRRVDLLDDPRFS
                     SAAARRLNFGALHQIIQSWMLTFPDMATLDAQFDEAKIAMGEIRSIKELTASEWSDYW
                     GAVQLVPDRSGGEYRLPGRPWRFSADELTPIGTPAFQGEHNIAVFSELGVGEAELKRL
                     AETGVLVTHRRALEPEMAVKPSTEPGQAA"
     gene            complement(3128..>3677)
                     /locus_tag="VGA15_27885"
     CDS             complement(3128..>3677)
                     /locus_tag="VGA15_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9591552.1"
                     /translation="NRRLSGVEFLRFMELLPIPLIASVHGVCLGGGLELALSCDYIIA
                     ASSAKIGSVEATLGLHPLLGGIQRQVQRIGALRAKEMSMLARRYDAPTLEKWGLINLT
                     VPEEQLEKATLAIAEEFAQGPTLAHAATKELVHIAVNDGVAAADEAMARVQAPIWASE
                     DLKTGLASFRKNGPGLAKFAGR"
BASE COUNT          680 a         1194 c         1121 g          682 t
ORIGIN      
        1 cgcgggccag attcatggcg gcgtggtgca gggaatagcc caagcccttt atgaagaggt
       61 cagctacgac cccgacaacg gacaattgct gaccggctcg ctgctcgact atgcggtgcc
      121 gcgcgccgag cacgtcccca acatcagatc attttttcag gagaccccct ccccgacgaa
      181 cccgatcggc gtcaagggca ttggcgaaag cggatcgatt gcggccccgc cgtgcatggt
      241 tcacgccgtg ctcgacgcat tgtcgccgtt tgggatatcg catctcgaca tgcccttgac
      301 gccgcctcga atctggtcgg cgattcagag cgcgcgcgcc ggagcaagcc aatgattccg
      361 gcatcatttg actatgtgcg cgcaacgtcg ctgtcccagg cgatcgattt gctgcagaac
      421 gatccggacg gaaccaggct cgtggccggc ggccacaccc tgatcccgac actgaagttg
      481 cggctggcgt cgccctcgct gctgatcgat atcggcagca ttgacgagct aagaggaatc
      541 gagaccggcg gtggccggat cagaattggc gcgctgacga cgcatgccga actgctcgca
      601 tccgggaaac tgcgcgaggt gttgccgatc tttggtcagg ccgccaacct gatcgccgac
      661 ccgcaagtcc gcaatcgcgg caccatcggc ggctccctgg caaatgcgga tcccgcggcg
      721 gactggcctg cggttgttat cgcgctgaag gcggaactcg agatcgcggg tccgaaaggc
      781 cgccgccgcg ttgcggcaac cgacttcttt gtggacatct ttaccacggc cctggaagcc
      841 ggcgaggttc tggcagccat ccatatcgcc cggcccgaac cggggacgcg gttcacatac
      901 cggaaaatcc gccatccagc gagcgggtat gccgtcgtcg gggtcgcggc tggtctccgc
      961 ctgaaggatg gcgtcgtcac ggagatttcg atcggcgtca ccggcgcggc aggtcgcgct
     1021 ttcgcagccg atgcggccac ggatttcctg agcggcaaga cgccttcgcg cgagaatatc
     1081 gaaacggccg ctgcattggt tagcgagaat accgactgct tgtccgatcg ctacgcctcg
     1141 gcggaatatc gcgccaacct cgtcaaaacc gaaatcgcgc gggcgttggc gtcgctactt
     1201 gcgctttcgc cgtgataaaa cacgggactt cgtgccggct ttagccggcc cagcatcggc
     1261 ggccccgcca aatctcttgc ggttgaaagc caccgcgccg gcgcccgcga cgacctcgac
     1321 gtcttcgagg cggagcggct tgccgctggc tgtcaggagt tttggatatt tgatctctcg
     1381 tccggcggcg cgatccctga aaaccacttg cggcccctcc ggctcggcca gccagtcgtc
     1441 accccatttc ttcagcgcca gataggccgg aaagaagtcg cgcccctttt ccgtcagcac
     1501 atactcgtat cgcccggagt gttccggcaa cgggatgcgg gtcatcacgc ccgcatcgac
     1561 aaatttcttc agacggctgc ttaggatgtt gggagccaca ccgacataat actcgaactc
     1621 gtcgaatcgc ttcacgccgt aataggcctc gcgcagaatc aagatagacc agcggtcgcc
     1681 gaccacttcc atggcgcgag ccatggaaca ttttttgttg gcgatacggc ttcgcttggc
     1741 gtcggacatt ggtttccttg gaccgggatt cctgctcaag cagtatatcc gagcccgacg
     1801 cccgcctgtc cagcgtcctg ctgaactgct ccaggaattg aggggcaaat acctgcggaa
     1861 tcaggcagcc tgtcccggtt ccgttgaagg tttgaccgcc atctctggct ccagcgcacg
     1921 gcgatgcgtg acgagcacgc ccgtctccgc cagccgcttc aactcggctt cgccgacgcc
     1981 gagttcgctg aaaaccgcga tattatgttc gccctgaaat gccggcgttc cgatcggcgt
     2041 caactcatct gcggaaaacc gccatggccg gcccggcagc cgatactctc cgccgcttcg
     2101 atccggcacg agttgcacag cgccccagta gtcgctccac tcggacgcgg tcagctcctt
     2161 gatcgagcgg atttcgccca ttgcgatctt ggcctcgtcg aactgggcat cgagcgtcgc
     2221 catatcgggg aatgtcagca tccacgactg aatgatctgg tggagtgcgc cgaaattcaa
     2281 ccgccgcgcg gccgcactgg aaaaccgcgg gtcatccagc agatccacgc ggcgcatcgc
     2341 gcgcagccag gacggaaatg tccggctgcc gatgatgctg gtcgcgacgg tgaattgctc
     2401 cccctgcggt ccggtgaaga acgaacaatc ggtggcgccg agcaccgcgg gctccgcccc
     2461 aatatcgtcg tcggacagat cgacatgcgc gcgttcattg accgcaagca aggtcgcggc
     2521 catcgcgacg tcgatgtatt ggcccaggcc ggttttctgg cggctgttca gcgccgcgag
     2581 gatcgcgatc accgcctgca atcccgcata gacgtcggcg tgcgacaggc tgtcggtgcg
     2641 cggttcagtg agcgcgctgc cataatggcg aacgctgttt tcggtgaagc cggcttcggc
     2701 ctgcaccgcc ggcgcataag ccatccggct gcgccacgga ccgccctggc cgtagcccgt
     2761 gatcgaggcg tagatcaatc gcggattgcg tttggaaagg gtctcgtaat cgagaccgaa
     2821 gaaaccgagc gttcccgccc ggaaattttc caccacgata tcggcggtat cgcagagctt
     2881 caacgccagc tcgtaggcgc cggggatatt gagattgatg ctgacgttgc gcttgccggc
     2941 gttctgctgg gcgtaatagc ccgacatgcc gtcggtcgat ggaaacgcaa agcgcgaaac
     3001 atccgggctt ggcggctcga ccttgatgac ctcggcgccg agatcctgca atgtccgcgc
     3061 gcacaggggg ccggccagca cgcgggaaaa gtcgacgacg cggattccat tgagaggacc
     3121 gctcatgtca gcgccccgcg aacttggcaa ggccaggccc gttcttgcgg aacgatgcaa
     3181 ggccggtctt cagatcttcc gatgcccaga tcggcgcctg gacccttgcc atcgcttcgt
     3241 cggcggcggc gacgccgtcg ttgacggcga tatgcacaag ctccttggtc gcggcatgtg
     3301 ccagcgtcgg gccttgcgca aactcctcgg cgatcgccag ggtcgctttc tcgagctgtt
     3361 cttccggcac cgtgaggttg atcagacccc acttctccag cgtcggcgca tcgtagcgcc
     3421 gtgcgagcat cgacatctcc ttggcgcgca gcgccccgat ccgctggacc tgacgctgga
     3481 ttccgccgag cagcggatga agccccaacg tcgcctctac cgagccaatc ttcgcggacg
     3541 acgcggcaat gatgtagtcg caggacagag cgagctcaag ccctccaccg aggcaaacgc
     3601 cgtgaacgct ggcgatcagc gggatcggca gcaattccat gaagcgcaag aattcgacac
     3661 cgctcagccg gcgattc
//
LOCUS       DASWGM010001035         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1938256,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001035 DASWGM010000000
VERSION     DASWGM010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938256"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1561
                     /locus_tag="VGA15_27890"
     CDS             410..1561
                     /locus_tag="VGA15_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HEX9591553.1"
                     /translation="MLAKPESACFVIADISGYTGFLAGVELEHAQDIIADVMDTMVRR
                     LRPPFRLAKFEGDAAFFYAVADKVDGSVLQDAVEAAYFAFRKRLRNISQATSCACNAC
                     SRMQDLDLKFVCHHGEFIKQKMSGRDELAGRDVILVHRLLKNGVNERLGGHAYALYSD
                     PCIRAMGIDPAAQGLVEHRESIDIIGEVTCWVRDLEAAWQQEKDRQMNQVTREAAAAV
                     IEFDVAAPRPAVWEHFTQPGLRPKWRAADEVRETSQSGRRGVGTVNHCMHGPHAIIEE
                     VLDWRPFDYLTISTLLPMPDAPKVIMSYAFLEEKPGATHIEIRLARPKPKDQAFLDHV
                     GAEFQKTITAEIEKLRQMLEGQDGAPAAVEEPMLPVPAERFLTEPVHAR"
BASE COUNT          321 a          523 c          538 g          297 t
ORIGIN      
        1 tcggcgcggg ccagacgccg ctagaccgtg cggtgaattg acgccggagc agcctgaaga
       61 gaattgctgg caagtcgctg ttcataccct gatgctatta aaaagccccg atatatcaag
      121 gcggccacta tcatgttgca gacggctctg aacttgagcg ggatgcttgc tcagcccccg
      181 gtgactgatg gtcagctatt ttaagctgcg cccattcctc ggcatagcgg acatggccgg
      241 acttgctgct ggctcgaccc ggtaacgttt gacccatctg agacatcgaa cctgatcgcg
      301 tctgaggccg gttgggtgcc atttccctcc cgctggccgg cacaaagtgc taggctttta
      361 agtattgcac aaggcgcttt ctcaggagtt acgaggaagg gacagctcga tgcttgcgaa
      421 acccgaatcc gcttgtttcg tgatcgcgga catctccggc tacaccggct ttctcgccgg
      481 ggtcgagctc gagcatgcgc aggacatcat cgccgacgtg atggacacca tggtgaggcg
      541 gctgcggccg ccgttccggc tcgccaagtt cgagggcgac gcggccttct tctacgcggt
      601 cgccgacaag gtcgacggct cggtgctgca ggacgccgtg gaggcggcgt atttcgcctt
      661 ccggaagcgt cttcgcaaca tcagccaagc gacctcctgc gcgtgcaacg cctgcagccg
      721 gatgcaggat ctcgacctca aattcgtctg ccaccacggc gagttcatca agcagaagat
      781 gtcgggccgc gacgaactcg ccggccgcga cgtcatcctg gtgcaccgcc tgctgaagaa
      841 cggcgtcaac gagcgcctcg gcggccacgc ctacgcgctg tattccgacc cctgcatccg
      901 ggccatgggc atcgatccgg cggcgcaggg cctggtcgag caccgggagt cgatcgacat
      961 catcggcgag gtgacgtgct gggtgcgcga cctcgaagcg gcatggcagc aggaaaaaga
     1021 ccggcagatg aaccaggtga cccgcgaggc cgcggcagcc gtgatcgaat tcgacgtcgc
     1081 cgctccgcgt ccggcggtgt gggagcattt cacccagccc gggctgcggc cgaaatggcg
     1141 ggcggcggac gaggtgcgcg agacttcgca gagcggccgg cgcggggtcg ggaccgtcaa
     1201 tcactgcatg catggtcccc acgccatcat cgaggaggtg ctggactggc gtccgttcga
     1261 ctaccttacg atttcgacgc tgctgccgat gcccgacgcc cccaaggtga tcatgtccta
     1321 cgcgttcctg gaggagaagc ccggggcgac gcatatcgag atccggctgg cgaggccgaa
     1381 gccgaaggac caggcgtttc tcgatcatgt cggcgcggaa ttccagaaga ccatcacggc
     1441 cgaaatcgaa aagctccggc agatgctaga aggacaggac ggggcgccgg ccgcggtcga
     1501 agagccgatg ctgccggtgc cggcggagcg cttcctgacc gagccggtcc acgcccgctg
     1561 acttatcgac ctagcgtggc gatgaggagg gctctattac accgaccgcg cgcttcataa
     1621 gcattgcggt ttcaaaccct ggcaaactgc cgatcaatga aaagccactt ccgctcatg
//
LOCUS       DASWGM010001036         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1642811,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001036 DASWGM010000000
VERSION     DASWGM010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642811"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VGA15_27895"
     CDS             <1..769
                     /locus_tag="VGA15_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX9591554.1"
                     /translation="AGFIGLEFAATARAKGLEVDVVELGARVMARAVTAEISDYFQER
                     HTAAGIRIHLGVQATSIESDGTNVTGVSLSDGRHVPADLVVVGVGVLPNVELAAEAGL
                     PVAAGIIVDEQLLTSDPNISAIGDCALFASPRFGGSLRLESVQNATDHARCVAARLTG
                     DARTYDGQPWFWSDQGDDKLQIAGLTTGYDRVVVRGDRGQRSFSAFCYNAGQLVGVES
                     INRGSDHVFGRKILGMHRSIEPEQAADLSFDLKAALA"
     gene            complement(873..1814)
                     /locus_tag="VGA15_27900"
     CDS             complement(873..1814)
                     /locus_tag="VGA15_27900"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX9591555.1"
                     /translation="MGRVFVAGSINMDVVATADRHPRIGETIAGKAVLYFPGGKGANQ
                     AVAAAKLGASTTLIGRLGRDAFGDELKAFLVAQGIDLSFVQQTSDTHTGTAIITVANA
                     DNTIVVIPGANALVSAADVEAPTLATGDIAVSQFEIPLPTITAFFQRARAAGTTTILN
                     PAPAVEFGRELLDLVDILILNETELGLLAKAELRDTDDHARFIDAARSLQAGQEKIIC
                     VTLGKRGIVALIDGQPHVDLGRAVKAIDTTGAGDCFVGAVAALLADGQPLRSAFRYAN
                     IAASICVQRMGAAPSMPTVEEVKAVLSASRPGEGRDP"
BASE COUNT          342 a          594 c          653 g          390 t
ORIGIN      
        1 cgcaggattc atcggcctgg aattcgccgc caccgcccgg gccaagggcc tcgaggtgga
       61 tgtggtcgag ctcggagccc gcgtgatggc gcgcgcggtg accgcggaaa tctcggacta
      121 ctttcaggag cgccacaccg ctgcgggcat tcgtatccat ctcggtgtgc aggccaccag
      181 catcgagagc gatggcacca acgtgaccgg cgtcagcctc agcgacggcc gccatgtgcc
      241 cgccgatctt gtcgtggtcg gcgtcggggt tctgcccaac gtcgaactgg cagcagaagc
      301 cggtctgccg gtggcggcgg ggatcatcgt cgacgaacaa ttgctgacgt cggatcctaa
      361 catctccgcg atcggcgact gcgcgctgtt cgcaagcccg cgcttcggcg ggtcgctgcg
      421 gctggagtcg gtgcagaacg ccaccgatca cgcgcgctgc gttgcggcgc ggttgactgg
      481 cgatgccagg acctatgacg gccagccgtg gttctggagc gatcagggcg acgataagct
      541 gcagatcgca gggttaacca ccggatacga tcgcgtcgtg gtgcgcggcg atcgcgggca
      601 gcgatcgttt tcggcgttct gttacaatgc cgggcagctc gtcggcgtcg agtcgatcaa
      661 tcgcgggtca gaccatgtgt tcggacggaa aattctgggc atgcaccggt cgatcgagcc
      721 ggaacaggcg gcggatttga gcttcgattt gaaggctgcg ctggcgtagg tttttttgaa
      781 catgtcgtcc cggcgaaggc cgggacccat acggcgcgga ctttcgtttt ggtagagtgg
      841 ctaacgcctt ctgctatcat caacgccagg ggttatgggt cccggccttc gccgggacga
      901 ctcgcggaga ggacggcctt gacttcctca accgtcggca ttgacggcgc ggcccccatt
      961 cgctgcacgc aaattgaggc tgcaatattg gcgtacctaa atgcactgcg aagtggctgt
     1021 ccgtcggcga gcagcgccgc gaccgcgccg acgaagcaat cgccagcgcc ggtggtatcg
     1081 atcgccttga cggcgcgacc gagatcgacg tgcggttggc cgtcaatcag cgccacgatc
     1141 ccgcgctttc ccaaggtgac gcaaatgatt ttctcttggc cggcttgcag cgagcgcgcc
     1201 gcgtcgatga atcgcgcatg atcgtcggta tcgcgcagct cggccttcgc caacaagccg
     1261 agttcggttt cgttgaggat gagaatatcg acgagatcca ggagttcgcg gccgaattcg
     1321 accgcgggcg ccggattgag gatcgtggtt gttcctgccg cacgggcgcg ctggaagaag
     1381 gcggtgatgg tgggcagcgg aatttcgaac tggctgaccg cgatatcgcc tgtcgcgagc
     1441 gtgggcgcct caacatcggc ggcactcacc agcgcattgg cgccgggaat gacgacaatg
     1501 gtgttgtcgg cattggcgac ggtaatgatg gccgttccgg tatgggtatc ggaagtctgc
     1561 tgcacgaagc tcaggtctat tccctgggct acgaggaacg ccttcaattc atcgccgaac
     1621 gcatccctgc cgagccggcc gatcagcgtc gtcgatgcac cgagcttcgc cgccgcgacc
     1681 gcctggttcg cgcctttgcc accgggaaaa taaagcacgg ccttgccggc gatcgtctcg
     1741 ccgatgcggg gatgccggtc ggccgtcgcc accacatcca tgttgatgct gccggcgacg
     1801 aagacgcggc ccattgccta atcccaaaat tccttcttac gcccgatctg aaactcttgc
     1861 ttgacgtttt cgatgtcggc aagcgtcggc aatccggcct cgttgccgag gcgttggatc
     1921 ttgtatgtcg aggccgcgcg cgcaaattcg aaatggtcgt gccagctttt gccgggatt
//
LOCUS       DASWGM010001037         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_544066,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001037 DASWGM010000000
VERSION     DASWGM010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544066"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VGA15_27905"
     CDS             complement(<1..241)
                     /locus_tag="VGA15_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX9591556.1"
                     /translation="MDAGTSPERPFQRTINTRCCIVGGGPAGMMLGYLLGRAGIDTLV
                     LEKHADFFRDFRGDTVHPSTLEVMDELGLIEGFLKL"
     gene            complement(267..1712)
                     /locus_tag="VGA15_27910"
     CDS             complement(267..1712)
                     /locus_tag="VGA15_27910"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX9591557.1"
                     /translation="MALAAPAHAEKRVALVVGNNDYKNVPKLQKAVNDARTMGATLKQ
                     LGFSVMVAENQTRQAFSQTLLAFDNAVEPGDTAFFFYAGHGFEIAGQNFLLPTDVPAA
                     GEGQEELVRDASVLADRIIERLQNKKVRTSILVFDACRNNPFERAGTRAVAGGGGLAP
                     MTQLPEGVFSVFSAGPRQTALDRLSNADDNPNSVFTRTFSKELLQPGENLVQVAQHTR
                     RLVSEMAETVSHKQIPVYFDQMVDDVFLNGMAKGQPEAAKPAEPTQKVAALPPVSVPR
                     IPQNDSINAPIAMFSRHNGGWTVVFSIADPTLGISWRMGDKGEFRETGFMDILDPRTR
                     KRMPNLSIELPGDAPAATIQVRYIDTSGEMQGPFPIKFDPEAALIRDQRKILDMTATS
                     WLSFREFNGLLVYYTHLMSYRCAIREVRVGIDTTVPDKVLKMPPCDPRDPSAIPSDAQ
                     PYLKLAPSTQSVSVELTYRDGSVSEIKSFRR"
BASE COUNT          481 a          779 c          769 g          479 t
ORIGIN      
        1 gaagtttcag gaatccctcg atcaggccga gttcgtccat cacctcaagc gtcgagggat
       61 gcacggtatc gccgcgaaaa tcccggaaga agtcggcgtg tttctccagc accagcgtgt
      121 cgatgccggc gcggccgagc aaatagccga gcatcatgcc ggcgggaccg ccgccgacga
      181 tgcagcatcg ggtgttgatc gtgcgctgga atggtcgctc aggagaagtg cccgcgtcca
      241 tgatctgact ttcgtcgaga ccgctctcag cgccggaaac tcttgatctc cgagacgctg
      301 ccgtcgcgat aggtcaattc caccgagacc gattgggtgg acggcgccag cttcagataa
      361 ggctgcgcgt cgctgggaat agcgcttgga tccctgggat cgcagggcgg cattttcagc
      421 accttgtccg gtaccgtcgt atcgatgccg acgcgcacct cgcgaatcgc acagcgatac
      481 gacatcagat gggtgtaata gactaggagg ccgttgaact cgcggaacga cagccagctg
      541 gtcgcggtca tgtcgagaat ttttcgctga tcgcggatca gcgccgcttc cggatcgaat
      601 ttgatcggaa acggtccctg catctcaccc gaagtatcga tatagcgaac ttgaatggtc
      661 gccgccggcg catcgccggg aagctcgatc gatagattgg gcatccgctt gcgggtgcgc
      721 ggatccagga tgtccataaa gccggtctcg cggaattcgc ccttgtcccc catccgccag
      781 gaaatcccca gcgtcggatc ggcgatcgag aacaccacgg tccagccgcc attgtggcgg
      841 gagaacatcg cgatcggcgc gttgatcgaa tcattttggg gaatgcgcgg caccgacacc
      901 ggcggcagcg ccgccacctt ctgcgtcggc tcggcaggtt ttgcggcttc aggctggccc
      961 ttcgccatgc cattcaggaa tacgtcgtcg accatctggt cgaaatagac cgggatctgc
     1021 ttgtggctga cggtctccgc catctcgctg accagccggc gcgtgtgctg ggcgacctga
     1081 accagattct cgccgggttg cagcagctct ttggaaaatg ttcgcgtgaa caccgaattg
     1141 ggattgtcgt cagcgttgga gagacgatcg agcgcggtct ggcgggggcc tgccgaaaac
     1201 accgagaaga cgccctccgg caattgtgtc atcggcgcga ggcctccgcc gccggcgacg
     1261 gcgcgggtgc ctgcccgctc gaacggatta ttgcgacagg cgtcgaacac caggatggag
     1321 gtgcgcactt tcttgttttg caggcgttcg atgatgcggt cggcgagcac ggaggcgtcg
     1381 cggaccagtt cttcctggcc ttcgcccgcg gcgggcacgt cggtcggcag caggaaattc
     1441 tgacccgcga tctcgaagcc gtggccggca tagaagaaga acgcggtgtc gcccggctca
     1501 accgcattgt cgaacgccag cagggtctgg ctgaacgcct gccgcgtctg gttttccgcg
     1561 accatcacgg aaaagcccag ttgtttgagg gtggcgccca tggtgcgggc atcgttgacc
     1621 gccttctgca gtttcggcac gttcttgtag tcgttgttac cgacgacaag cgcgacccgt
     1681 ttttccgcgt gcgccggcgc tgcgagcgcc atcagcgcga ctgcgacgct tgtcagcgtt
     1741 ggtagcccgg aaagccatct cgtcatcgaa ttccccacct cgcacgattg acccgcggct
     1801 cgcgccaacc gccttatgcc gtagtcggca aacctgcgcc gccggttcaa tcgagcaccg
     1861 cagcccggcg ccccaccgcc gagcagcggc ccggcaaagt tcagggcgag tgccgatagc
     1921 gccagccgcg gattgacaca actcagcgca atcgcaagca gcgatgagcc catcagcatg
     1981 atcgccgaca tctgccgatg acgcaaatgg tcgcccagtt ccagatgcgg cgtgatcaag
     2041 acaagacgaa ggccgatggc agcaatcagc gccgtatgac tcgtatagcg ccagatcgcc
     2101 gccgtgaact gcgcaaaacc gccgacgacg atagtcgtga acggggcgca gccgacaaca
     2161 cgggcaagga cagtgtcaag cttcgcatgg gcagcaggac aaccggaacg ggtggataga
     2221 atgtggcgcc atccatgcaa aacattaagc tttttcgccc ttccgccgcc cgctccattg
     2281 actttggcca attcgcccct atgttccgct tcgacgcggc ccggtatcga aacgcgatat
     2341 cttgaataag ccgcctgcct gcgtttccga aaaccccgtg caaatctgcg tgccgttccg
     2401 gggttgagcg cgacagtctt ttccgggatt ccaagcggcg gtatcttcca gaggctcaat
     2461 gtctttttcc catctcggct tgtccgacaa ggtgctcgcc gccgtcgc
//
LOCUS       DASWGM010001038         3256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_261101,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001038 DASWGM010000000
VERSION     DASWGM010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261101"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2315
                     /locus_tag="VGA15_27915"
     CDS             <1..2315
                     /locus_tag="VGA15_27915"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX9591558.1"
                     /translation="KRNEDRRLLTGQALFIDDIELPGMLHVAFLRSQVAHAKIVSIDV
                     SSALQRPGVVAAFVAGDLGDYWHPGPLLVPPPPIAGIVFHQRTQVPLAKDKVRHVGEP
                     LVIVIAESRYIAEDALDDIDVELDPLPAVVDLEKGLAGGSALVHDDVGSNVSAHVRQT
                     KGNYAAAAAKADHIIKRRFLYEHGISSPIETRGVVAQWDKRANQMTIWDTTQAPVFVR
                     NGLAGMLGLNERQVRVIAPFVGGGFGPKIMMFYPEEVLLPWVSMKLNRPIKWIEDRLE
                     HFFATTHERGQIHDAEIALTKDGRILGIKDVFLHDGGAYDPYGLTVPINSQCTLLGPY
                     VVPNYDSTFTQVFTNLPIVTPYRGAGRQHGVFVMERLLDFAARELNIDATEIRRKNLI
                     PPEAFPYKNEIIYQDFQPLEYDSGNYEPVLDMALEAIGYRAFKEEQVAARLQDRHVGI
                     GLACYVEGTGIGPYEGAKVQVQPNGKVSVATGIGTQGQGHFTVFAQIAADQLGVAVTD
                     VDVVTGDTDQFYWGAGTFASRGAVVAGNAVNEASKVVRKKALKLASDLFECAEDDLVI
                     EDGKVSIVGIPGKFVKLGDLARQANPMRGAVQPGTEPGLESTQYFGPKMGATANGVHA
                     MIIEMDPQTFDLKILKYIVVHDCGTVINPMILAGQIHGGVAQGIGNAFYEKLVFDDQG
                     QLLNASLADYLLPTALEVPRMELGHTVTASPLNPMGIKGAGEAGAIPAGPLFAQAIED
                     ALDLKGRGVELLEIPLSPSRLFELTRNAQR"
     gene            2342..3202
                     /locus_tag="VGA15_27920"
     CDS             2342..3202
                     /locus_tag="VGA15_27920"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEX9591559.1"
                     /translation="MILVTGAGGKTGKAVLKALVARGEAVRAFVRSAAHHAALKAIGV
                     HNVELGEMDDPRAWSQAIRGTSAVYHICPNVNPHELPFAKALVAAAGTHGIRRFVYHS
                     VLHPQIEAMPHHWAKLRVEEMLFSSGLDVTILQPTAYMQNSLAEWDRMVADGIYRVPY
                     PVETKLSLVDLDDVAEAAALVLTEDGHSGATYELAGTPPMSQIEIAETFGHALRRAVR
                     AEADPVEVWTQRARGAAMDDTQLETLAKMFQAYARDGLKGNPNVLGWLLKRPPTSLAA
                     FAARMAAAHA"
BASE COUNT          633 a         1027 c         1013 g          583 t
ORIGIN      
        1 tcaaacgcaa tgaagatcgc aggcttctga cgggccaggc gctgtttatc gacgacatcg
       61 agttgccggg gatgctgcat gtcgcgttcc tgcgcagcca ggtcgcgcat gcgaagatcg
      121 tcagcatcga tgtttccagc gcactgcagc ggcctggcgt cgtcgcagcc ttcgtcgccg
      181 gcgacctcgg cgattactgg caccccggcc ctttgctggt gccgccgcct ccgatcgccg
      241 gcatcgtctt ccatcagcga acccaggttc cgctggcaaa ggacaaggtc cgccatgtcg
      301 gcgagccctt ggttatcgtg atcgccgaga gccgttacat cgccgaggac gcgctcgacg
      361 atatcgacgt cgaactcgat ccgcttccgg ctgtggtcga tctcgaaaag gggctcgctg
      421 gcggatcggc gctggttcac gacgacgtcg gctccaacgt ctccgcccac gtgcgtcaga
      481 ccaagggcaa ctatgccgca gctgcggcaa aggcggatca catcatcaag cgtcgctttc
      541 tctacgagca cggcatctcg tcgccgatcg agacccgcgg cgtcgtcgcg caatgggata
      601 agcgcgccaa ccagatgacg atctgggata cgacacaggc gccggtgttc gtccgcaacg
      661 gccttgccgg catgctcggc ctcaacgaac gtcaggtccg cgtcatcgcg ccattcgtcg
      721 gcggcggctt cgggccgaag atcatgatgt tttatcccga ggaggtgctg ctgccgtggg
      781 tatcgatgaa actgaaccgg ccgatcaaat ggatcgagga ccggctcgag catttctttg
      841 ccaccaccca tgagcgtgga cagattcacg acgccgaaat cgccctgaca aaggatggcc
      901 gcatcctcgg catcaaggac gtgttcctgc acgacggcgg cgcctacgat ccgtatgggc
      961 tgaccgtgcc gatcaacagc cagtgcacgc tgctcggccc ctatgtcgtg ccgaattacg
     1021 acagcacgtt tacgcaagtg ttcaccaatc tgccgatcgt cacgccgtat cgcggcgccg
     1081 gacgccagca cggcgtgttc gtgatggagc gactgctcga cttcgcggcc cgtgaattga
     1141 acatcgacgc gaccgaaatc cggcgcaaaa atctgattcc gcccgaggca ttcccttaca
     1201 agaacgagat catctaccag gatttccagc cgctcgaata tgacagcggc aactacgagc
     1261 cggtgctcga catggcgctg gaagcaatcg gctacagggc cttcaaggag gaacaggtcg
     1321 cggcccgact tcaagatcgt catgtcggca ttggtctcgc ttgctacgtc gaaggcaccg
     1381 ggatcggtcc ctacgaaggt gccaaagtgc aggtgcaacc caacggcaag gtgagcgtcg
     1441 ccaccgggat cggcacccag ggacaggggc acttcaccgt gttcgcgcaa attgccgccg
     1501 accagcttgg cgtcgccgtc acggatgtcg atgtcgtcac cggcgatacc gatcagttct
     1561 attggggcgc cggcaccttc gccagccgcg gcgccgtggt cgcgggcaac gccgtcaacg
     1621 aagcctcaaa ggtggtgcgg aagaaggcgc tgaagctcgc gagcgacctg ttcgaatgcg
     1681 ccgaggacga cctcgtgatc gaagacggca aggtgtcgat cgtcggcatt cccggcaaat
     1741 tcgtgaagct tggcgatctt gcgaggcagg ccaatccgat gcgcggggcg gtccaacctg
     1801 gcaccgagcc gggactggaa tcgacgcagt atttcgggcc caaaatgggg gcgacggcca
     1861 atggcgtcca cgccatgatc atcgagatgg acccgcaaac gttcgacctg aagatattga
     1921 aatacatcgt ggtgcatgat tgcggcaccg tgatcaatcc gatgattctg gccggccaga
     1981 ttcatggtgg cgtcgcgcaa gggatcggca atgcattcta cgaaaagctg gtgttcgacg
     2041 atcagggcca actgctcaac gcctcgctgg cggactatct gttgccgacc gcgctcgagg
     2101 tgccgcgcat ggaactcggc cacaccgtga cagcgtcgcc gctcaatccg atgggcatca
     2161 agggcgccgg cgaggccggt gccatccccg ccgggccgct gtttgcgcaa gccatcgagg
     2221 atgcgctcga cttaaagggg cgcggcgtcg aactgctgga gattccgctc agcccgagcc
     2281 ggctgttcga actcacccgg aatgcgcagc gctagactag cgcaaaatcg gggtgccaca
     2341 catgatcctg gtcactggcg ctggcggcaa gacgggcaag gccgtgctta aggcactggt
     2401 tgcgcgcggc gaggccgttc gtgcatttgt gcgaagcgcc gcgcatcacg cagccttgaa
     2461 ggcgatcggc gttcataacg tcgaactcgg cgagatggat gatccgcgcg catggtcgca
     2521 ggccattcgc ggaacgagcg cggtctatca catctgcccc aatgttaatc ctcacgagct
     2581 gccatttgca aaagccctcg tcgcagccgc cggcacccat ggtatccggc gatttgtcta
     2641 tcactcggtg ctgcatccgc agatcgaggc gatgccgcat cattgggcca agctgcgggt
     2701 cgaggagatg ctgttcagct cagggcttga tgtcaccatc ctgcagccca cggcctacat
     2761 gcagaattcg ctcgccgaat gggaccgcat ggttgccgac ggcatctatc gcgtgcccta
     2821 cccggtcgaa acgaaactca gccttgtcga tctcgacgac gtcgccgaag ccgcagccct
     2881 ggtgctgacc gaggacggcc attccggcgc gacctatgaa ctggccggca cgccgccgat
     2941 gagccagatc gagattgccg agacgtttgg acacgccttg cggagagccg tccgcgccga
     3001 ggccgatccg gtcgaggtct ggacccaacg cgcccgcggc gccgcgatgg acgacaccca
     3061 gctcgaaacg ctggcaaaga tgtttcaggc ctatgcgcgc gacggtctca agggtaatcc
     3121 caatgtgctg ggctggctgc tcaagcgccc gccgacgtcg ctcgcggcct ttgccgcgcg
     3181 catggccgcc gctcacgcgt gaggcggcca gccaatgggg tgggtctgct gcacgatctt
     3241 catggccgat aaaatc
//
LOCUS       DASWGM010001039         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1939519,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001039 DASWGM010000000
VERSION     DASWGM010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939519"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..>2135
                     /locus_tag="VGA15_27925"
     CDS             72..>2135
                     /locus_tag="VGA15_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9591560.1"
                     /translation="MDRLVAIAVSRRYLMVGLFAAVIVGGLIAFKQLNIEAYPDPTPP
                     MVDIVTQSPGLSSEEIERYITIPIETQVSGIKNLRTIRTISLYGLSDVKLQFSFDYTY
                     DEALQQVLNRLSQLPPLLGNVQPGISPVSPIGEIYRYRLAGPPNYSVLDLKTLQDWVL
                     QRRFRAVPGVIDVTGWGGRTKTYELQVDFNKLIAYGLTLPQLLQAVGNSNINVGGNTV
                     NLGAQSAVVRGVGLIRSIDDLASTMVSQSGGNPVLVKDIATVTVGEKPRLGIAGMNQD
                     DDIVQGTVLMRRGEQSSPTIARVEALVASINNSSILPPGVRIERIYDRKDLIDLTTAT
                     VLHNMVVGILLIVLLQWIFLGDLRSALIVGATIPFALFLAVIILVLRGESANLLSVGA
                     IDFGLIVDATVILVEAIFRRLSQTEAEQSYISTDTAMGMKSHAILSASADVSRSIFFA
                     AAIIIAAFLPLFTLGGVEGNIFGPMARTYAYALAGGLLATFTVTPALSAIILPSHIHE
                     TETWIMALLHRLYVPVLNWAVGNRRPVMAGAVGLAVMTLVFGRLLGLEFLPKLEEGNL
                     WIRATLPPTISLQEGNAFVNEMRKTIRGFPEVESVVSQHGRPDDGTDAAGFFNAEFFA
                     PLKPVSEWSGAHDKDELTAQLLAKLQDKFPGVEFNFSQYLQDNVSEAVSGVKGENSIK
                     LYGNDL"
BASE COUNT          407 a          646 c          640 g          442 t
ORIGIN      
        1 gacgccacgt aaggagaact tcggattcgg gacacgggtc ctccttcggc ccattgaaag
       61 ctctgatctg aatggatcgc cttgtcgcca tcgcagtcag ccgccgttat ttgatggtgg
      121 gcttgttcgc cgccgtgata gtcggcggcc tgatcgcgtt caagcagctc aatatcgaag
      181 cctatcccga tccgacgccg ccgatggtcg acatcgtgac gcaaagtccg ggattgtcgt
      241 cggaagagat cgaacgctac atcaccattc cgatcgagac ccaggtgtcg ggcatcaaga
      301 acctgcgcac gatccggacc atctcgctgt acggactatc cgacgtcaaa ctgcagtttt
      361 cgttcgacta cacttatgac gaggcgctgc agcaggtgct gaaccggttg tcgcaattgc
      421 cgcctttgct tggcaacgtg cagccgggga tttcgccggt cagccccatc ggcgaaatct
      481 atcgctaccg gctggcgggg ccgccgaact acagcgtgct cgacctgaaa acgctgcagg
      541 actgggtttt gcagcgccgc ttccgcgccg tacccggcgt gatcgacgtc accggctggg
      601 gcggaaggac caagacctac gagctgcagg tcgatttcaa caagctgatc gcgtacggcc
      661 tgacgctgcc gcaactgctg caggccgtcg gtaattccaa catcaatgtc ggcggcaata
      721 ccgtcaatct cggcgcgcaa tccgccgtgg tgcgtggcgt cggcctgatc cgttcgatcg
      781 acgatctggc cagcaccatg gtgtcgcaga gcggcggcaa tccggtgctg gtcaaggata
      841 tcgccacggt gaccgtcggc gaaaagccgc ggcttggcat cgccggaatg aatcaggacg
      901 acgacattgt gcagggcacg gttctgatgc ggcgtggtga gcaaagttcg ccgaccatcg
      961 cgcgggttga agcgctggtg gcctccatca acaactccag cattttgccg ccgggagtgc
     1021 gcatcgagcg tatctacgac cgcaaggatc tgatcgacct caccaccgcg accgtgctgc
     1081 acaatatggt ggtcggcatt ctcctgatcg tgctgctgca atggattttc ctcggcgatc
     1141 tgcgcagcgc gctgatcgtt ggtgcgacca ttccgttcgc gctgtttctc gccgtcatca
     1201 ttctggtgct gcgcggcgaa tccgccaacc tgctctcggt cggcgccatc gatttcgggc
     1261 tgatcgtgga tgccaccgtg atcctggtgg aggcaatttt ccgccggctg tcgcaaacgg
     1321 aagccgagca aagctatatc tcgaccgaca ctgccatggg catgaaaagc cacgccattc
     1381 tcagcgcgtc cgccgacgtt tcgcggtcga tcttctttgc agccgccatc atcatcgcgg
     1441 cgttcctgcc gctgttcaca ctaggcggcg tcgaaggtaa tatcttcggg ccgatggcgc
     1501 ggacctacgc ctacgcgctg gctggcggcc tgcttgcgac cttcacggtc acaccggcac
     1561 tgagcgcgat catcctgccg tcgcatatac acgagaccga aacctggatc atggcgctcc
     1621 tgcatcggct ctatgttccg gtattgaatt gggcggttgg caaccggaga ccggtgatgg
     1681 ctggagccgt ggggctcgcg gtcatgaccc tcgtgttcgg ccgcctgctg ggactggaat
     1741 tcttgcccaa actcgaagag ggcaatctct ggatccgcgc gaccttgccg ccgaccatct
     1801 cgctgcagga gggtaacgcc tttgtcaacg agatgcgcaa gaccatccgc ggctttcccg
     1861 aggtcgagtc ggtggtgtcg cagcatggcc gccccgatga cggcaccgac gctgccggat
     1921 ttttcaatgc ggaattcttt gcgccgctga agccggtcag cgaatggtcc ggggcgcacg
     1981 acaaggatga actaacggct caattactgg cgaagttgca ggataaattc cccggcgtcg
     2041 aattcaactt ctcgcaatat ctgcaggaca acgtttccga ggcggtatca ggcgtaaagg
     2101 gagagaactc gatcaaattg tacggcaacg atctg
//
LOCUS       DASWGM010001040         8615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1504156,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001040 DASWGM010000000
VERSION     DASWGM010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1504156"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="VGA15_27930"
     CDS             <1..648
                     /locus_tag="VGA15_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEX9591561.1"
                     /translation="VIGYTAIQSMAKWARRNDMILHLHRAGHSTYTRQRNHGVSFRVI
                     AKWMRLAGVDHIHAGTVVGKLEGDPATTRGYYDICREDFNPAKLEHGVFFDQSWASLN
                     KLMPVASGGIHAGQMHQLLDHLGEDVVLQFGGGTIGHPMGIQAGATANRVALEAMILA
                     RNEGRDYLHEGPEILAKAAATCTPLKAALEVWKDVTFNYESTDMPDYAPTASVSM"
     gene            661..1086
                     /locus_tag="VGA15_27935"
     CDS             661..1086
                     /locus_tag="VGA15_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEX9591562.1"
                     /translation="MRVTQGCFSFLPDLTDEQISRQVQYCLRKGWAVNIEFTDDPHPR
                     NNFWEMWGLPMFDLRDAAGVMMELNECRKVYGDRYIRMSAFDSSHGWESQRLSFIVNR
                     PKDEPGFRLERHEVNGRNIRYTTKSYAVDRPEGVRYGDR"
     gene            1083..2018
                     /gene="cbbX"
                     /locus_tag="VGA15_27940"
     CDS             1083..2018
                     /gene="cbbX"
                     /locus_tag="VGA15_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HEX9591563.1"
                     /translation="MNSQLSSSSIPQSAGETPPVEQVDLRREFAEVGIGEVLDQLDRE
                     LIGLKPVKTRIREIASLLLIERIRKRMNLTSDVPTLHMSFTGNPGTGKTTVALRIASI
                     LHKLGFVRRGQVVSVTRDELVGQYIGHTAPKTKEILKKAMGGVLFIDEAYYLHRPDNE
                     RDYGQEAIEILLQVMESQREDLVVILAGYGDRMDKFFASNPGFRSRIAHHIDFPDYSE
                     DELLAIAELMLHDMNYRFSPDARQAFIHYISLRKTQPLFSNARSIRNALDRMRLRQAN
                     RLVADLDRVLTADDIMSLEAPDVLASRVFSKNSSG"
     gene            complement(2114..3907)
                     /locus_tag="VGA15_27945"
     CDS             complement(2114..3907)
                     /locus_tag="VGA15_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9591564.1"
                     /translation="MERRLAAIVCADVAGYSRMMGADEQGTHAAFKAHRSATYPIILN
                     YGGRVVKNTGDGFLLEFPSIVGAIESSIAMQALMAERNNHLPADRVMQFRLGVHMGDV
                     IADEDEVYGDGVNIAVRLEAVAAPGGVAVSAKAYHEASKHLRATLVDAGSHRLKNIEE
                     PVNVWTWEPGGSDTRGREPKNTSNLPAQYRTAIVGVLPFANLSDSTDEYFSDGLTEDL
                     IHALSLQSFYRVLSRNSTFSFKDKNLSARLIAREIDATYLIQGSVRRAGTKIRVTAEL
                     VAPENGEQLWTGRYDRDIGDLFAMQDEITTSLSAALAPEIYRAEASAPARSSSTDLTA
                     WDRFLRGLSHYYRHTKADFEASISLFREAIALDPALAIARAYLATIMVQGVQYGWLKS
                     TSQLWTEAMNLAESSVRLDPRSSFAFSILSYMHAMQGHYEAAMEAAKRAVKLNPYDMG
                     ARGVLGISHLVIGEHRQAIELFSAAVQRGNSDPRYKWAALNAFSHYLVGQYDASLSWA
                     REALYLNPNHLQVLAVRAAALAQLGRTEEAAKAAEVLLTNFPGLTVERHLRNFRWKTP
                     ADIAHFRDGLLKAGVPFSKLTLVDSTSKRTA"
     gene            complement(4141..4845)
                     /locus_tag="VGA15_27950"
     CDS             complement(4141..4845)
                     /locus_tag="VGA15_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HEX9591565.1"
                     /translation="MATHPRYAIYYAAAPDTDLDRFGARLLGCDAFSGKDLPFPDGVL
                     QMAPDWRELTRDPRKYGFHATLKPPLSLAPGKTETELLAACEAFATTPRPVPVIRPVV
                     DSISGFIAVVPAEPSTELERLAADCTSEFDSFRAPLTPEGRTRRDPTALTERQCGHLD
                     RWGYPYVMEDFRFHMTLTGRLDAERREPVLRMLRNSFSAIGIKTLAIDRIALFRQVDA
                     DSRFRIVSHWKLRVRR"
     gene            complement(4866..6059)
                     /locus_tag="VGA15_27955"
     CDS             complement(4866..6059)
                     /locus_tag="VGA15_27955"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HEX9591566.1"
                     /translation="MTDILIEGGRALLGNTILETSVQIAGGKISAVGSDHGVISFGLD
                     ARGLLVLPGIVDLHGDAFERQMMPRPGVDFPVDVALIDSDRQAIGNGITTVFHATTWS
                     WEPGLRSGDNATRLLEAIETLRPQLAADTRFHLRHETYNLDAETDIAQWLSGGRIDLF
                     AFNDHMDSTVTSLAKPQKRNRMVERTGLSNEAFDSLVERVVSRGDDVPASVTRLAKAA
                     RYANVRMLSHDDESPEMRKAFRAQGVSIAEFPVNEETAREAAEAGDFIVFGAPNVVRG
                     GSHTGWTKAADMIAKGLCSILASDYYYPAQLLAAFRLADDGVLPLAEAWNLISATPAR
                     AAGLADRGVLAEGHRADIILVDDELPLRPRIVAVIAAGRLVHLTDASRLIHSSVKPHK
                     AVAAA"
     gene            complement(6056..6724)
                     /locus_tag="VGA15_27960"
     CDS             complement(6056..6724)
                     /locus_tag="VGA15_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX9591567.1"
                     /translation="MAGKMLSVEPTMDPSAKLHDTTLGVYCEVGARTILHEVTLGDYS
                     YVVNDAQITYTTIGRFCSIAAMTRINPGNHPMHRASQAHFTFRASAYFPGESDEAEFF
                     EWRRQHYVHIGHDVWIGHGAIVLPGRSIGTGAVVAAGAIVTKDVPAYTIVAGNPARAI
                     KRRFSEDITNRLAELEWWDWDHEALRSALPDFRKLAVEEFLAKYEAATTSGHLSTRRR
                     SAVS"
     gene            6921..7736
                     /gene="phnC"
                     /locus_tag="VGA15_27965"
     CDS             6921..7736
                     /gene="phnC"
                     /locus_tag="VGA15_27965"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX9591568.1"
                     /translation="MLVVEGLTCRFGSKAAVDNASFSIAPGGFVGVIGRSGAGKSTLL
                     RMINRLADPSEGRILFEGLDVTTLRGKNLRQWRARSAMIFQQFNLVGRLDVLTNVLMG
                     RLADISSWRSLTQLWPEEDKALAMSALEQFDMAQLAAQRADQLSGGQQQRVAIARALV
                     QEPDIILADEPIASLDPRNTKIVMDALLRINKHFGITVICNLHSLDLARSYCDRLIGM
                     ASGRVVFDGAPAMLTDHIARELYDLEANEVMGGSAQQDPADSGIPALGTAAAA"
     gene            7834..>8615
                     /gene="phnD"
                     /locus_tag="VGA15_27970"
     CDS             7834..>8615
                     /gene="phnD"
                     /locus_tag="VGA15_27970"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9591569.1"
                     /translation="MINRRYILAGAAALAFAASAASAQDWKAKYPELTFAVVPAENAS
                     GVTERWTPFVAYLSKELGVKVTLRIANDYAAVIEGQRSGNIHIASYGSASFARARLTG
                     VKTDAFANDINSDGSTGYYSMFFVKASSPYKGIDELKGKNLGLVDPNSTSGNNVPRFE
                     LDKMGISDADSYFGKVVFTGSHENAMLALAQGTVDVAANQWTSDDDSTLAQMLHKGML
                     KNADGTPMKKEDFRIIHKSAQIINGPYAYSSELPEDLKAAIA"
BASE COUNT         1768 a         2577 c         2568 g         1702 t
ORIGIN      
        1 gtgatcggct ataccgcgat ccagtcgatg gcaaaatggg cgcgccgcaa cgacatgatc
       61 ctgcatctgc atcgggcagg gcattcgacc tatacgcggc aacgcaacca cggcgtctcg
      121 ttccgcgtga tcgcgaaatg gatgcggctc gcgggggtcg atcacatcca tgccggcacg
      181 gtggtcggca agctcgaggg cgatccggcc accacgcgcg gctattacga catctgccgg
      241 gaagacttca atccagcaaa actcgaacac ggcgtgttct tcgatcagag ctgggccagt
      301 ctcaacaagc tgatgccggt ggcgtccggc ggcattcatg ccggccagat gcatcagctg
      361 ctcgaccatc tcggcgaaga cgtggtgctg cagttcggcg gcggcaccat cggtcatccc
      421 atggggatcc aggccggtgc gaccgcgaac cgcgttgccc tggaagccat gattctcgcc
      481 cgcaacgaag ggcgcgacta tctgcacgaa gggccggaaa ttctcgccaa ggcggcggcg
      541 acctgcacgc cgctgaaggc cgcgctggaa gtctggaagg acgtcacctt caactacgaa
      601 tccaccgaca tgcccgacta tgcccccacc gccagcgtct cgatgtaagg agctaagatc
      661 atgcgtgtca cccaaggctg tttttcattc ctgcccgacc tcacggacga gcagatctcc
      721 cgccaggttc agtattgtct ccgcaagggg tgggcggtga acatcgaatt caccgacgat
      781 ccgcatcccc gcaacaattt ctgggaaatg tgggggctgc cgatgttcga tcttcgcgat
      841 gccgccggcg tcatgatgga gctaaacgaa tgccgcaagg tctatggcga ccgctacatc
      901 cggatgtcgg cattcgattc cagtcacggc tgggagtcgc agcggctgtc tttcatcgtc
      961 aaccggccga aggacgagcc gggcttccgt ctcgagcggc acgaagtaaa cggccgcaac
     1021 atccgctaca ccacgaaatc ctatgccgtc gatcgtcccg aaggcgtgcg ctacggtgac
     1081 agatgaattc gcaattgagt tcatcttcca tcccgcagag cgcaggcgaa acgccgccgg
     1141 ttgagcaggt cgacctgcgc cgcgaattcg ctgaggttgg catcggcgag gtactcgatc
     1201 aactggaccg cgaactgatc gggctaaagc cggtcaagac gcgaattcgg gaaattgcct
     1261 cgctgctgct gatcgagcgc atccgcaagc gcatgaacct gacgtcggac gtgccgacgt
     1321 tgcacatgtc gttcaccggc aatcccggca ccggcaagac gacggtggcg ctccggatcg
     1381 ccagcattct ccacaagctc ggctttgtac gccgcggcca ggtcgtgtcg gtcacccgcg
     1441 acgaattggt gggtcagtac atcgggcaca ccgcgccgaa gacaaaggag attttgaaga
     1501 aggcgatggg cggcgtgctg ttcatcgacg aagcctatta cctgcatcgc cccgataacg
     1561 aacgcgacta tggacaggaa gccattgaaa ttttgctgca ggtgatggaa tcgcaacgcg
     1621 aggatctggt ggtgatcctt gccggctacg gcgaccggat ggacaagttc ttcgccagca
     1681 atcccggctt ccgctctcgt atcgcccacc acattgattt tccggattat tccgaggatg
     1741 agttattggc gatcgcggaa ttgatgctgc acgacatgaa ctaccggttc agtcccgacg
     1801 cgcgccaggc gttcatccat tacatcagct tgcgcaagac ccagccgcta ttctccaacg
     1861 cacgctcgat ccgcaacgca ctcgatcgga tgcggctgcg ccaggccaac cgtctcgtcg
     1921 ctgatcttga ccgcgtgctg accgccgacg acatcatgtc gctggaggcg cccgatgtgc
     1981 tggccagccg ggtgttctcg aagaactcca gcgggtgaaa ccggtccgct cagatatcat
     2041 cgaggtgtaa cgcattggtt ccgtctggtc tcaagcccgc aacgtttgca gctaagtctt
     2101 tgagaaaccg cgcctaagcc gtgcgttttg aggtagaatc gacgagggtc agtttgctga
     2161 acggtacgcc ggccttcaaa agtccgtcac gaaaatgggc gatgtcagcc ggagttttcc
     2221 aacggaagtt cctgagatgc cgttcgacgg tcaggcctgg aaagttggtc agcaaaactt
     2281 cggccgcctt tgccgcttcc tcggttcgtc ccaattgcgc caaagccgcc gctcgtacgg
     2341 cgagcacctg aagatgattg ggatttagat acagggcttc acgcgcccag gacagcgacg
     2401 catcatattg tcctacaagg tagtggctga acgcgttcaa cgccgcccat ttataccggg
     2461 ggtcactgtt gccgcgctgc acggccgccg aaaacagttc gatggcctgt cgatgctcgc
     2521 cgatcacgag gtgagatatt ccaagtacgc cgcgggcacc catgtcgtac gggtttaact
     2581 taaccgccct ctttgcggct tccattgccg cctcgtaatg cccctgcatc gcatgcatgt
     2641 aggacaaaat cgaaaatgcg aatgacgaac gcggatcaag ccggacactg ctttccgcca
     2701 ggttcatcgc ctcggtccac aattggctcg tacttttgag ccatccatac tgcacaccct
     2761 gcaccatgat cgtggcgaga taggcgcgtg caattgcaag cgccggatcg agcgcaatgg
     2821 cctccctgaa cagactgatc gaggcttcga aatctgcttt tgtgtgccga taatagtgag
     2881 acagtcccct taagaatcga tcccatgcgg tcagatcggt ggacgatgac cgggccggag
     2941 cggaagcttc cgcacgatat atctccgggg ccaacgcagc ggacagactg gtggtgattt
     3001 catcctgcat cgcgaacaga tcgccgatgt ccctgtcgta tcgaccggtc cataattgct
     3061 cgccgttctc cggggcgacc agttcggcgg ttacacgaat tttcgttccg gcacgccgta
     3121 ccgaaccctg gatcaggtat gtcgcatcaa tctcccgcgc aatcagccgt gcgctcagat
     3181 ttttgtcctt gaacgaaaaa gtcgaatttc tgctcagtac gcggtaaaag gattgcaagg
     3241 aaagagcgtg gatcaagtcc tcggtgagtc cgtcggaaaa atattcgtcg gtgctgtcac
     3301 tgaggttcgc gaacggaagc acgcccacga tcgcagttcg atattgagcc ggcaggtttg
     3361 atgtattttt gggctcccga ccgcgggtgt cggacccgcc tggctcccag gtccagacat
     3421 ttaccggctc ttcgatgttc ttcaaacggt gactgccggc gtcgacgagg gtggcacgga
     3481 gatgcttgct ggcctcatga taggcctttg cggaaacggc cacgccgccg ggggcggcaa
     3541 ccgcttcaag gcggacggcg atgttgacgc cgtcgccgta aacttcgtct tcgtcggcga
     3601 tcacatctcc catgtggacg cccagacgga attgcatgac ccgatcagcg gggaggtggt
     3661 tattccgttc tgccatcagc gcctgcatcg cgatcgaaga ctcgatggcc ccgacgatgc
     3721 tcggaaactc cagcaggaaa ccgtcgccgg tgttcttcac aacacggccg ccgtagttga
     3781 ggatgatcgg gtatgtcgcg cttcgatgag ccttgaacgc ggcgtgcgta ccttgctcgt
     3841 ctgcgcccat catgcgcgaa tagccggcga catcagcgca gactatggca gctaggcgtc
     3901 tttccatctc ctggccatct cctaggcacc tgaactggca gatacggtac gtggtcggct
     3961 cccggaatcc aatggtttta aaacacactg cccatgcgga aacccgatct ggaggtagcg
     4021 gtaatgcaat ccgctccgga ttggcggggg ttggccgacg atctcgagga gcgggaaccg
     4081 gagaagtcca gggaggctga tcgcagcgac ttggccgcgg ccaacgcttg accttcgctc
     4141 tcagcgccta acgcgcagct tccagtggct gacgatgcgg aaccgggaat cggcgtcgac
     4201 ttgacggaat agcgcgatcc tgtcgatcgc gagcgttttg atgccgattg cggagaagct
     4261 gttacgcagc atcctcagaa cgggctcccg ccgttcggcg tcaagtcggc ctgtcagcgt
     4321 catgtggaag cggaagtcct ccataacgta cggatacccc cagcgatcga gatgtccaca
     4381 ctgcctttcc gtcaaggcag taggatcccg ccgcgtacgg ccttccggtg taaggggggc
     4441 ccgaaatgaa tcgaattcgc tggtgcaatc cgcagcaagc cgctcaagtt cggtcgatgg
     4501 ctccgcaggc accacggcga taaagccgct gatcgaatcg acgacaggcc ggatcaccgg
     4561 aactggtcga ggcgtggtgg cgaaagcctc gcatgctgca aggagctcgg tctcggtctt
     4621 gccaggcgcc agcgacagcg gcggcttcag cgtggcatgg aaaccgtatt ttcttggatc
     4681 ccgcgtcagt tcacgccaat ccggcgccat ctgcaggacg ccgtccggaa agggcaggtc
     4741 cttgccgctg aaggcgtcac aaccaagcaa tcgcgcgccg aagcgatcaa gatcggtgtc
     4801 cggcgcggct gcataataga tcgcataacg tggatgagtt gccatatcag cagaatagag
     4861 ccggctcacg cggctgcaac cgccttgtgt ggcttaacgg atgagtggat cagtcggctc
     4921 gcgtccgtca gatgaacgag acggccggcg gcgatgacgg cgacaatgcg cggccgcagc
     4981 ggcagttcgt catcgacgag gatgatgtcg gcacgatgcc cttcggcgag aaccccgcga
     5041 tccgctagac ctgcggccct ggcgggcgtc gccgaaatca ggttccaggc ttcggcaagc
     5101 ggcaacacac catcgtcagc aaggcgaaat gccgccagca actgcgccgg atagtagtag
     5161 tcggaggcca ggatcgagca taagcccttg gcgatcatgt cggccgcttt ggtccatccg
     5221 gtatggctgc cgccgcgcac gacattgggc gcgccgaaca cgatgaaatc gccggcttcg
     5281 gccgcctcac gcgcggtttc ctcgttgacc ggaaattcgg caatgctgac gccctgcgcg
     5341 cgaaatgcct ttcgcatctc cgggctttca tcgtcatgag acagcatccg cacgtttgcg
     5401 tatcgcgcag ctttggccag ccgggtcacc gatgcaggga cgtcgtcacc acgggacacc
     5461 acacgctcca ccagggaatc gaacgcttcg ttggaaagac cggtacgttc gaccatacgg
     5521 tttcgttttt gcggcttggc cagactggtg acggttgaat ccatgtggtc attgaacgcg
     5581 aacagatcga tccgtccgcc ggacaaccat tgcgcgatgt cggtctcggc atcgagattg
     5641 taggtctcat ggcgcaggtg gaagcgggta tccgccgcca gttgcggcct cagggtctcg
     5701 atggcctcaa gcaatcgcgt tgcattgtcg ccgcttctca ggcccggttc ccaggaccac
     5761 gtcgtggcgt gaaaaaccgt ggtgatgcca tttccgatgg cctgccggtc gctgtcgatc
     5821 agcgctacat cgacgggaaa atcgacaccg gggcgcggca tcatctgtcg ctcgaacgcg
     5881 tcgccatgca aatcgactat ccccggcaac accagcaggc cgcgggcatc gagaccaaat
     5941 gaaatgactc cgtggtccga acccactgcg ctgatttttc caccggcgat ctgcaccgag
     6001 gtttcaagaa tggtattgcc gagaagcgcc cggccgcctt cgatcaatat atccgtcacg
     6061 atactgcact tcgtcgtctg gtggataaat gaccggaagt ggtcgcggcc tcgtattttg
     6121 cgagaaactc ctcgacagcc agtttgcgga aatcgggcag ggcactgcga agcgcttcat
     6181 ggtcccagtc ccaccactca agctccgcca gccggttggt aatgtcctcc gaaaaacgcc
     6241 gcttgatcgc ccgcgccgga tttcctgcga caatcgtgta ggccggtacg tccttggtca
     6301 cgatcgcgcc agcggcgacc acggcgccgg tgccgatgct gcggcccggc aacacgatgg
     6361 cgccgtgccc gatccagaca tcatggccaa tatggacgta atgctggcgc cgccattcga
     6421 agaactccgc ctcgtcgctc tctcccggaa aataggcgct ggcgcgaaag gtgaagtgcg
     6481 cctgcgacgc gcggtgcatg ggatgattgc cgggattgat ccgcgtcatg gctgcgatcg
     6541 agcagaacct tccgatcgtg gtgtaggtga tctgcgcgtc gttcacgacg taggaataat
     6601 cgcccagcgt gacctcatgc aggatcgtgc gggcgccgac ttcgcaatac acaccgagcg
     6661 ttgtgtcgtg caattttgcg ctgggatcca tggtcggttc aaccgatagc attttgccgg
     6721 ccatgggaat tttccacaaa taaccgatcc caggatactg aaaaaggccc gcacgtcatc
     6781 gatggccttg atgacagagt catgacaaac gcttgacagt ctgccgaggt gtggatgatt
     6841 gccgcactgc agcgtgctgt cacacaactg tcgaatccag acgatagcgg tggtccagga
     6901 ttacgcgtct ggagcattac atgctggtgg tggaaggttt gacgtgccgg ttcggctcca
     6961 aggccgcggt cgacaatgcg tcattctcga tcgcgccggg cggctttgtc ggtgtgatcg
     7021 gccgttccgg ggccggcaag tcgactttgc tgcgaatgat caatcgcctc gccgacccat
     7081 cggagggacg aatcctgttc gaaggtctgg acgtgacaac gctgcgcggc aagaatctgc
     7141 ggcaatggcg cgcacggtcg gcgatgattt tccagcaatt caatctggtt ggccgccttg
     7201 atgtgttgac caacgttctg atgggacggc tcgcggacat ctcgtcctgg cgctcgctga
     7261 cacagctttg gcccgaagag gacaaggcgc tggcgatgtc tgcccttgag caattcgaca
     7321 tggctcagct ggccgcgcag cgtgccgacc agctctccgg cgggcagcag cagcgcgttg
     7381 cgattgcgcg ggcgctggtt caggagcccg acatcatcct tgccgatgag ccgatcgcgt
     7441 cgctggatcc ccgcaatacc aaaatcgtga tggatgcgct gctgcgcatc aacaagcatt
     7501 tcggcatcac cgtgatctgc aacctgcatt cgctcgatct cgcgcgcagc tattgcgacc
     7561 gtctgatcgg gatggcgtcc ggccgcgtgg tgttcgacgg ggcgccggcg atgttgaccg
     7621 atcacatcgc gcgcgagctt tacgatctcg aagccaacga agtgatgggc ggctcggccc
     7681 agcaagatcc agctgattcg ggaattccgg ctctaggcac ggctgccgcg gcctgactat
     7741 cgcgtacgtc aagacggctt gttgcaaaac tgcgatcagc cgatttgttg caattcgcta
     7801 caagcaattc gtcacaacaa gacagggaac gtcatgatca atcgtcgata cattctcgcc
     7861 ggtgccgcgg cgctggcgtt cgccgcttcc gccgcatcgg cgcaagactg gaaagccaaa
     7921 tatcccgaac tgactttcgc ggtggtgccc gcggagaacg cgtcgggcgt caccgaacgc
     7981 tggacgccgt tcgtcgccta tctgtccaaa gaactcggcg tgaaagttac cttgcgcatt
     8041 gccaacgact acgccgcggt gatcgaagga cagcgatccg gcaacatcca catcgcgagc
     8101 tacggttcgg cgtcattcgc gcgcgcaagg ttgaccggcg tcaagaccga tgctttcgcc
     8161 aacgacatca attccgacgg tagcaccggc tattattcga tgttcttcgt aaaggcgtcc
     8221 agcccgtaca agggcatcga cgagctgaag ggcaagaatc tcggcctcgt tgatccgaat
     8281 tcgacatccg gcaacaacgt tccccggttc gaactcgaca agatgggcat ttcggatgcc
     8341 gacagttatt tcggcaaggt cgtcttcacc ggcagccacg aaaacgcaat gctggcgttg
     8401 gcgcaaggca ccgtggatgt cgcggccaat caatggacga gcgacgacga ctcgaccctg
     8461 gcgcagatgc tgcacaaggg gatgctgaag aacgccgacg gcacgccgat gaagaaggag
     8521 gatttccgga tcattcacaa atcggcgcag atcatcaacg gaccctatgc ctatagttcg
     8581 gagcttccgg aagatttgaa ggccgccatc gccaa
//
LOCUS       DASWGM010001041        11573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1644270,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001041 DASWGM010000000
VERSION     DASWGM010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11573
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644270"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1449
                     /locus_tag="VGA15_27975"
     CDS             262..1449
                     /locus_tag="VGA15_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX9591570.1"
                     /translation="MRRKTAVRAGLLLVVLAGGGAFAFTHGFGSKTSAPKPSAPTPVP
                     IVAGTVEQHDVPIYLTGVGTVVAYNTDVVRSQIQGQIVSINFTEGQAVHAGDLLAQID
                     PRPYQAQVDQYTANRDRDEAQLTNAKANLNRYAPLAQKGWATPQLLDTQKAQVAQLEA
                     AVKSDEALIESANVQLSYTRLTSPIDGVTGIRQIDVGNIISPANTNGVVIVTQLEPIS
                     LIFTLPETVLPLIQQQQQQKTQEPLAVLAYSQDNTMRLAQGTLGLVNNEILQTTGSIQ
                     LKANFPNKAHKLWPGELINARLLLDTRHDGLTVPASVVQQGPKGAYAYVVNPDSSVAI
                     RPIKVAQVSDGQALIDSGLTANEQVVVDGQYKLQPGTHVTILHGQAAEEAAAQSAQQA
                     PIP"
     gene            1446..4559
                     /locus_tag="VGA15_27980"
     CDS             1446..4559
                     /locus_tag="VGA15_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX9591571.1"
                     /translation="MNISAPFIQRPIATALLMVGLLVGGLAAYPLLPVASLPNVNYPT
                     LQVTAQLPGADPQTMASSVATPLELQFGEIPGLTQMTSASALGFTQVTLQFDLSRGID
                     GCVSDTLSAINAATANLPPNMPYPPTIRKVNPADTPILVLGVTSDSLPLTTVDAYAEN
                     ILLQKISQISGVGLVGIGGQQKPAIRVQLDPEALASRGISLEDVRNVLGQANVDLPKG
                     TLNSPRQTYTLNTNDQLLKADEYDNLVIAYSNGSPVRVRDVGRAISAPENNLIAGWFN
                     TQRAIILVIQRQPGANVIETVNRVKAMLPVLQASIPPAVKVSIISDRTETIRASVADV
                     QFTLMLTVALVVMVIFVFLRNFWATIIPAVTVPLSLVGTFAVLYELGYSLDNLSLMAL
                     SIAVGFVVDDAVVVIENIVRHMEEGASPFEAALKGAGEIGFTIVSITLSLIAVFIPLF
                     LMSGYVGLLFREFAITVSVALILSLLISLTLTPMMCSRLLKPETKEHGWLYRLFERGF
                     DGLLNAYEAGLKVVLRHRFVTMMVMLGTIALTGYLYVVIPKGFFPQQDTGLIIGLSEA
                     AQDISFQAMAERQQALLNAVMQDPAVASIGSGVGAGGGNTTVNNGRVYIALKPQNQRP
                     SMDQVLARLRANLAKIQGITLYMQAAQDITIGGRVSKTQYQYTLDDADPGELNHWAAL
                     FLEKIKAVPGVTDVATDQLNAGPKLDITIKREVASSYGILPFTIDNTLDDAFGQRIVS
                     TMFTTLNQYHVVMEVDPKFQYGPEALNGIYVKSSSGQQVPLSTLVDSVVTVAPLVVNH
                     QGQFPSVTISFNLAPGTAIGKAVSAIQQIEKDLGKPLSLQTSFQGNAQAFGDSLSSTP
                     ILIAAALFVIYLILGVLYESVIHPITIISTLPSAGLGALLLLMAVHYDLSVIAIVGII
                     LLIGIVKKNGIMLVDFAQQVEHSEGLTAEESIYRACVLRFRPILMTTMAALLGGVPMM
                     LGTGVGSEIRQPLGYAIVGGLALSQILTLYTTPVVYIYLDRLQTWMFGGERKQAVSGH
                     AEPVPAE"
     gene            4852..6096
                     /locus_tag="VGA15_27985"
     CDS             4852..6096
                     /locus_tag="VGA15_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate decarboxylase family bicupin"
                     /protein_id="HEX9591572.1"
                     /translation="MISRREVLAATAAGAVMSAATARAASFGNPDEPPQGAINAKNPG
                     SVTDPGPQNPAVVGQFPSAVSPPATDVGGMPMTWASFNNAPRRIQNGGWARQVTQADF
                     AISETIAGVNMRLAVGGIRELHWHQAAEWAFMTYGNCRVTVLDTNGRPYVTDVKEGDI
                     WYFPAGYPHSLQGLGPDGCEFVICFDNGAASEFNTLLVTDWFIHTPPSVLAKNFGVPA
                     ETFAKMPLHDLWIFQGKVPGELSAVKKAMTGKAGAPPYPFTFSLGSSAPVSETKSGKV
                     QIADSNNFNVSTTVAAALVTVRPGALREMHWHPNADEWQYYVKGKGRMTVFNTGPNAL
                     TMDFNPGDIGYVKRNYGHYVQNVGDSDLQFIGVFRAPRYEEFALSDWLTHTPPALVAQ
                     HLNVDETTIAKWPNDRPAVLPE"
     gene            6235..6465
                     /locus_tag="VGA15_27990"
                     /pseudo
     CDS             6235..6465
                     /locus_tag="VGA15_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
     gene            6670..7266
                     /locus_tag="VGA15_27995"
     CDS             6670..7266
                     /locus_tag="VGA15_27995"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEX9591573.1"
                     /translation="MRKNALKPVATHGVSSGQPRLHQRGREIQAFGTVRRSLLGLSDE
                     ARLYSCQRLNQLLADTRILHALYKNHHWVMRGPGFYQLHLLFEKHADEQIELVDEIAE
                     RIQALGGVAVGDPRHVAEITRIPRAPNGCEAVPAMLSRLLEAHELILLDGHDAATSFT
                     ERGDDGTKDLIVSEVIRIGDTQVWSLAEYLANAPLVRG"
     gene            7375..8598
                     /locus_tag="VGA15_28000"
     CDS             7375..8598
                     /locus_tag="VGA15_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX9591574.1"
                     /translation="MASPMMTRRSLLGTAGLGGLALLSAPAWAQRMIDLPLPGGPDAR
                     SITTQFPQKGSMILQRTRPPLLETPWDVFDKGVFTPNNQFYVRWHWAGIPTSVDIDTF
                     RLTVHGHVQRTVSLTLKDMLDGFSRVELAAVNQCSGNSRGFFAPRVPGAQWVNGAMGN
                     ALWTGVRLKDVLDRAGVKAGAVQARFSGLDEAVVPDAPRLMKSLDVDHTRDGEVMIAY
                     AMNGEQLPLLNGFPLRLVVPGWYSTYWVKMLNDIEILDQPDTNYWMKTAYTIPDTPHA
                     SIRPGDTGVKMVPINRMVPRSFLTNVSPGNTVPVGAPTPARGIAFGGDCGVAKVDISS
                     DAGKSWNATQLGKDEGKYGFRQWETRITLPSAGRYTLMVRCTNTNGEAQPDTPNWNPA
                     GFMRNVIESVDVIAA"
     gene            8643..8942
                     /locus_tag="VGA15_28005"
     CDS             8643..8942
                     /locus_tag="VGA15_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9591575.1"
                     /translation="MGAALAPFAACAAAPLELKSVTVDLPDSDRTFPPGPGSDAVNNN
                     CLACHSAGMVLNQPALSKAAWSAEVNKMITAYKAPVAPEDVGAIVEYLTSLPSAK"
     gene            9197..9535
                     /locus_tag="VGA15_28010"
     CDS             9197..9535
                     /locus_tag="VGA15_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX9591576.1"
                     /translation="MIICSCNVLSDHDVRRVMTAAASQPRTASDLYGCLGCSAQCGRC
                     ARTIKRIMDEALSASSCCCRKTSLPPAPVVRAGDLRTGSSSWSASRHGGNRSARKSGS
                     TGPNWGSPSG"
     gene            9451..10551
                     /locus_tag="VGA15_28015"
     CDS             9451..10551
                     /locus_tag="VGA15_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEX9591577.1"
                     /translation="MVRIAARWKSLGTQVREHRAQLGLSLRVTVAALLSFVLSHLLHV
                     PLPLWTVLTAVILTQVSFGKSLKATIDYLVGTLGGAVYAGAVAALFPHANEIALAGVL
                     AIAVAPLALLGAINPRFSVATFTGVLVILVPEITHVSSIASAFYRVLEVALGGLTAVA
                     VSFLVLPARAHVLAIEAAAQMLDLMARSLPELFGGFMQARDATAIGRIQDNIGQALAR
                     LEALVAEARHERISLLAAEPDPGPLLRTLLRLRHDLVMIGRAAAVPLPEPFQGRFGPL
                     LAQIANSAADYLRRSGEALAAGRDPPSLDAAEAALDDYADAFTIVRHEGLTRGLPADT
                     AERIFALGFALDQLHQNFRDLERWVREAVAGR"
BASE COUNT         2154 a         3755 c         3573 g         2091 t
ORIGIN      
        1 cgttttcgtc gtcgccctcg acgatctctc ccggcggcgc cggccgcacc ggaatcccga
       61 tgggctcgac cgagatcgga aacgcgggaa ttagcggcgt gcccgccgtc acgacgccga
      121 gccccgccat ttccaacatc ctgccgggga gctccaattg tcccacgaat ggaatgtcca
      181 acgcccaaaa ccccggagca atcccgtcaa cgacgggctt tccgggcgtc tgctgaaccg
      241 acatcgcagg gggagagcgt catgcgacga aagacggcgg ttcgcgccgg cctgctgctc
      301 gtcgtcttgg cgggcggcgg tgctttcgcg ttcacgcacg gatttggatc caaaacatcc
      361 gcaccgaagc ctagcgcgcc gacgccggtg ccaatcgtcg ccggaacggt cgagcagcac
      421 gacgtgccga tctacctgac cggcgtcggc acggtggtcg cgtacaacac cgacgtcgtg
      481 cgcagccaga tccagggcca gatcgtcagc atcaatttca ccgaagggca ggcggtgcac
      541 gccggcgacc tgctcgccca aatcgatccg cgtccgtacc aagctcaggt cgaccagtac
      601 accgcgaacc gcgatcgcga tgaggcccag ctcaccaatg cgaaagccaa tctcaaccgc
      661 tatgcgccgc tggcgcagaa aggctgggcg actccgcagc tgctcgacac ccagaaggcg
      721 caggtcgcgc agctcgaggc ggccgtcaaa tccgacgagg cgctcatcga atcagcgaac
      781 gtgcagctca gctacacgcg gttgacttcg ccgatcgatg gcgtgaccgg cattcggcag
      841 atcgacgtgg gcaacatcat cagtccagca aatacgaacg gcgtcgtgat cgtcacgcag
      901 ctcgagccga tttcgctgat attcacgctg ccggaaaccg ttctgccgct aattcagcag
      961 cagcagcagc agaaaaccca ggaaccgctc gcggttctcg cctacagcca ggacaacacg
     1021 atgaggctcg ctcagggcac gctcggcctc gtcaacaatg agatcctgca gaccaccggc
     1081 tcgatccagc tcaaggcgaa ttttcccaac aaggcgcata agctttggcc gggcgagctc
     1141 atcaacgcgc ggctgctgct cgacacgcgg catgacggcc tcaccgttcc cgcatcggtc
     1201 gtccagcagg gcccgaaagg cgcctacgcg tacgtggtca atcccgacag ctcggtggca
     1261 atccgcccca tcaaggtcgc gcaggtcagc gacggccagg cgctcatcga ttccggcctg
     1321 acggccaacg agcaggtcgt ggtcgacgga caatacaagc tgcagcccgg cacgcatgtc
     1381 acgatcctgc acggccaggc ggccgaggaa gccgccgccc aaagcgcgca gcaggcgccg
     1441 atcccatgaa catctccgcg ccgttcatcc agcggccgat cgcgaccgcg ctgctgatgg
     1501 ttggattgct ggtcggcggt ctggccgcct acccgctgtt gccggtggcg tcgctgccca
     1561 acgtcaatta tccgacgctc caggtcaccg cgcagctgcc gggcgccgac ccgcagacca
     1621 tggcatcctc ggtggcgaca ccgctcgagc tgcagttcgg cgagatcccg ggcctcaccc
     1681 agatgacgtc ggcgagcgcg ctcggcttta cccaggtcac cctgcagttc gacctgagcc
     1741 gcggcatcga tggctgcgtc agcgacacgc tctccgcgat caatgccgcc accgcgaacc
     1801 tgccgccgaa catgccgtat ccgccgacga tccggaaggt caacccggcc gacacgccga
     1861 tcctggtgct cggcgtgacc tcggacagcc tgccgctgac cacggtcgac gcttatgcgg
     1921 aaaatatttt actgcaaaag atctcgcaga tctccggcgt cggcctcgtg ggtatcggag
     1981 gccagcagaa gccggccatc cgcgtacagc tcgatccgga ggcgctggcc tcccgcggca
     2041 tcagcctcga ggacgtgcgc aatgtgctcg gccaggcgaa cgtcgatctg ccgaaaggca
     2101 ccctcaacag cccgcgccag acctacacgc tcaacaccaa cgatcagctg ctcaaggccg
     2161 acgaatacga caatctggtc atcgcctaca gcaacggctc gccggtccgc gtgcgcgacg
     2221 tcggccgcgc catcagtgcg ccggagaaca atctgattgc cggctggttc aacacgcagc
     2281 gggcgattat cctggtgatt cagcgccaac ccggagccaa cgtcatcgag acggtgaacc
     2341 gggtcaaggc gatgctgccg gtgctccaag cgtcgatccc tccggccgtc aaggtttcga
     2401 tcatttccga ccgcaccgag accatccgcg catcggtcgc cgacgtgcag ttcacgttga
     2461 tgctcaccgt tgcgctggtg gtcatggtga tcttcgtgtt cctgcgcaat ttctgggcaa
     2521 cgatcattcc cgccgttacc gtgccgctgt cgctggtcgg cacgttcgcg gtgctctacg
     2581 agctcggata cagcctcgac aacctctcgc tgatggccct gtcgatcgcg gtcggcttcg
     2641 tggtcgacga cgccgtcgtc gtcatcgaga acatcgtgcg gcatatggag gagggcgctt
     2701 caccgttcga ggcagcgctg aagggcgccg gcgagatcgg cttcaccatc gtgtcgatca
     2761 cgctgtcgct cattgcggtg ttcattccgc tgttcctgat gagcgggtat gtcggactgc
     2821 tgtttcgtga gttcgcgatc acggtgagcg tcgctctcat tctctcgctg ctgatctcgc
     2881 tcaccctcac cccaatgatg tgctcgcggc tgctcaagcc ggagaccaag gagcacgggt
     2941 ggctctaccg gttgttcgaa cgcggcttcg acggcctgct caacgcctat gaggcaggcc
     3001 tgaaggtcgt gctgcggcac cgctttgtca ccatgatggt aatgctcggc accatcgcgc
     3061 tgaccgggta tctctacgtg gtgataccca agggcttctt tccccagcag gacaccggat
     3121 tgatcatcgg cctctccgag gcggcgcagg atatctcctt ccaggccatg gcggagcgcc
     3181 agcaagcttt gctcaatgcc gtgatgcagg atccggccgt cgcctccatc ggttcggggg
     3241 tgggtgccgg cggcggcaac accacggtca acaacggccg cgtatacatt gcactcaagc
     3301 cccagaatca gcgccccagc atggaccagg tgctcgcgcg gcttcgtgcc aacctcgcca
     3361 aaatccaggg gatcacgctt tacatgcagg ctgcgcagga catcaccatc ggcgggcggg
     3421 tgtccaagac ccagtaccag tacacgctcg acgatgccga cccgggcgag ctcaaccatt
     3481 gggcggcgct cttcctcgag aagatcaagg ccgtcccggg cgtcaccgac gtcgccaccg
     3541 atcagctcaa tgccgggccg aagctcgaca tcaccatcaa gcgcgaggtc gcatcgagct
     3601 acggcatcct gcccttcacc atcgacaaca ccctcgacga cgccttcggc cagcgcatcg
     3661 tgtcgaccat gtttaccacg ctgaaccagt accatgtcgt catggaggtc gatcccaagt
     3721 tccagtacgg gcccgaggcg ctcaacggca tttacgtcaa atcatcgagc gggcagcagg
     3781 tgcccttgtc cacgttggtc gacagcgtcg tgacggtggc gccgctcgtc gtcaaccacc
     3841 aaggtcagtt tccctcggtg acgatctcgt tcaacctcgc gcccggcacc gcgatcggta
     3901 aggcggtgag cgccatccag cagatcgaaa aagacttggg caagccgctc tcgctgcaga
     3961 cgagcttcca gggcaacgcc caggcattcg gagactcgct gtcgagcaca ccgatcctca
     4021 tcgccgcagc gctgttcgtc atctacctaa ttcttggggt gctctacgag agcgtgatcc
     4081 acccgatcac gatcatctca accctgccgt cggccgggct cggcgcgctg ttgctgctga
     4141 tggcggtgca ctacgatctg agcgtgatcg ccatcgtcgg catcatcctg ctcatcggca
     4201 tcgtcaagaa gaacggcatc atgctggtcg attttgccca gcaggtcgag catagcgagg
     4261 gtttgacggc cgaggaatcg atctaccgcg cctgcgtgct gcgctttcgg ccgatcctga
     4321 tgaccacgat ggcggcgctg ctcggcggcg tgccgatgat gctgggcacg ggggtcggct
     4381 cggagattcg ccagccgctc ggctacgcca tcgtcggcgg cctggcgttg tcgcagattc
     4441 tcacgctcta caccacgccg gtcgtctaca tctatctcga ccggctgcag acctggatgt
     4501 tcgggggaga aaggaaacaa gcggtctccg gccacgccga gccagtgccc gccgaatgac
     4561 cggcaggggt cgcccgggtg gccgacgaat tcggtagaag tggacaaatc acggcaatcc
     4621 acgaaaagct attttccggt gcaaccatta ttttcgatag catctggcag cggctactac
     4681 ccggaagcca acccgttgct cccgttggca taccacgatg caaagagcaa aaccccggcg
     4741 gcgaaatcga tcccggttgt ggtcagacca caatgagggg atgggcaacg tatgtcatgc
     4801 ggaacccgct ggacgggctt cgtccctccg ctcgagagga gtcaatgggt catgatatcg
     4861 agacgcgaag tactggcagc gacagccgct ggtgcggtga tgagtgccgc cacggcgcgc
     4921 gcggcctcct tcggcaatcc ggacgagccg ccgcaaggag cgatcaacgc caagaacccg
     4981 gggagcgtca ccgatccggg cccgcagaat cctgcggtcg tcggtcagtt tccctcggcg
     5041 gtatctccgc ctgcgaccga cgtcggcggc atgccgatga cctgggcatc cttcaacaac
     5101 gcgccacggc gcatccagaa cggcggctgg gcgcgtcagg tcacacaggc cgacttcgcg
     5161 atttccgaga cgatcgccgg cgtcaacatg cgactggcgg tcgggggcat ccgcgaattg
     5221 cactggcacc aggcggccga atgggcgttc atgacctacg gcaactgccg ggtcactgtt
     5281 ctcgacacga acggccggcc ctatgtgaca gatgtgaagg agggggacat ctggtatttc
     5341 cccgccggct acccgcattc gctgcagggg ctcggccccg acgggtgcga gttcgtcatt
     5401 tgcttcgaca atggagccgc gagcgagttc aacacccttt tggtgacgga ctggttcatc
     5461 catacgcctc cgtcggttct cgccaaaaac ttcggtgtgc ccgccgagac gttcgcgaaa
     5521 atgcctctgc acgatctctg gatttttcag ggcaaagttc cgggagagct gtctgctgtc
     5581 aaaaaggcga tgaccggaaa ggcgggcgct ccgccgtacc cgttcacgtt ctctctcgga
     5641 tcatcggcac ccgtcagcga gaccaaaagc ggcaaggtgc agatcgccga cagcaacaat
     5701 ttcaacgttt ccacaacggt tgctgcggcg ctcgtgaccg ttcgacccgg agcgttgcgg
     5761 gagatgcatt ggcatccgaa cgccgacgaa tggcaatatt acgtcaaggg caaggggcgc
     5821 atgacggtgt tcaacaccgg tcccaatgcg ctcacgatgg acttcaaccc gggcgacatc
     5881 ggttacgtca agcgtaacta cggacattat gtacagaacg tcggcgacag cgatctgcag
     5941 tttatcggcg tgttcagggc tccccgttac gaagaattcg ccttgtcgga ctggctgacg
     6001 catacgccgc cggcgttggt cgcccagcac ctgaacgtcg acgagacgac gatcgcgaaa
     6061 tggcccaacg accggccggc agtcctgccc gagtgatcgg atcgggcgcg cggcatggct
     6121 tttgcggaga tcgacgtgcc gaacttgccg actgcggtcg cgtgcctgcc gcgcaccgcg
     6181 gcgcgcggcg ccacctggta gggggcgagc ggctcgttcc cgaggagacc gcgattggtc
     6241 gtcctgatct tcctggcggc cttcgccacc ctggccatct cattctggcc gtacatgatc
     6301 ccgttcgtcc tcgccatcga cgaagcggcg gcgcctcaat cgaggttcat gttctggggc
     6361 gagggcctgt tcgtcttccc gctgatgctg ctctacacga tcatcagcta cagagtcttc
     6421 aggggcaaag tcgcatccac gcccgagcac tctgagactg cttaggttcg acggacaaga
     6481 gaggcggatt gtgcgggggc caattgaaca atggcggcaa catcggcacg accacggcaa
     6541 tacgcatcaa gcgtttttcc ggcattcgga taggcttccc agctcccgca tgcccaactc
     6601 agcgcccccg caggtgcgcc gggtcggacg caaacagcgc ttcactgatc attcgcaagg
     6661 agcagccgca tgagaaagaa cgcactgaaa ccggttgcca ctcacggcgt gtcgagcggt
     6721 cagccccggt tgcaccagcg cggcagggag atccaagcgt ttgggaccgt gcggcggtct
     6781 ttgctcgggc tatctgatga ggcgcggctg tattcctgcc aacggctcaa tcagctcttg
     6841 gccgacacgc ggattctcca cgcgttgtac aagaaccacc attgggtgat gcgaggacct
     6901 ggcttctatc agctgcactt gctgttcgag aagcatgcag atgagcagat cgagctggtc
     6961 gacgagatcg ccgaacggat acaggccctg ggcggagtcg cggtgggcga tccccggcac
     7021 gtcgccgaga tcaccaggat cccccgggca ccgaacggct gcgaggcggt gcccgcgatg
     7081 ctgtctcggc tgctggaggc gcacgagctg atcctgctcg acggacacga cgcagctacg
     7141 agcttcaccg agcggggcga cgatggcaca aaagacctga tcgtctccga ggtgatccgc
     7201 atcggagata cccaggtttg gtccctcgcc gagtatctcg ccaacgcccc gctcgtccgt
     7261 ggctgagact aagccgcgcg atttgtgctc gatggcacga cctatgagga gaagaggcgc
     7321 acgatcgtgc cgaacggccc ggcaagcagg ttgcctcgaa taaccgataa aggtatggct
     7381 tctcccatga tgacacgtcg gagcctgctg ggcacggcag gtctaggcgg gttggcactc
     7441 ctatccgcac ctgcctgggc gcagaggatg atcgatttgc cgctgcccgg cggtcccgac
     7501 gcgcgatcga tcacaaccca atttccccag aagggttcaa tgatcctgca gcggacgcga
     7561 ccgccgctgc tggagactcc ttgggatgtc ttcgacaagg gtgtgttcac gccgaacaat
     7621 caattctatg tgcgttggca ttgggccggt atcccgacct cggtcgatat cgataccttc
     7681 cgcctgacgg tgcacggtca tgtgcagcga accgtgtcgc tgacgctcaa ggatatgctc
     7741 gatgggttct cacgcgtgga attggccgcc gtgaatcaat gctcgggcaa ttcgcgcgga
     7801 ttctttgcgc cgcgggtgcc gggcgcccaa tgggtgaacg gcgccatggg gaacgcgttg
     7861 tggaccggcg tccgcctgaa ggacgtgctc gatcgggcgg gtgtgaaggc cggcgccgtg
     7921 caagcgcgct tcagcggcct cgacgaggcg gttgtgccgg acgcgccgcg cctgatgaag
     7981 tcgctcgacg tcgaccacac gcgcgacggc gaggtcatga tcgcctatgc gatgaacggc
     8041 gaacaactcc ccttgttgaa tggctttcct ttgcggctcg tcgttccagg ctggtactcg
     8101 acctattggg tcaagatgct gaacgacatc gagattctgg accagcccga caccaactat
     8161 tggatgaaga cggcctacac cattccggat acgccgcacg ccagcattcg gcccggcgac
     8221 accggcgtga aaatggttcc catcaaccgg atggttcccc gatccttcct cacgaacgtc
     8281 agccccggca acacggtgcc tgtcggcgcg ccgacaccgg cgcgtggcat agcattcggc
     8341 ggcgactgcg gcgttgcgaa ggtggacatc tcgagcgatg caggcaaatc ctggaacgcg
     8401 actcagctcg gaaaggacga ggggaagtac ggcttccggc agtgggaaac ccggatcacg
     8461 ctgccgtcag caggcagata cactttgatg gtccgctgca cgaacaccaa cggcgaggcc
     8521 cagcccgata cccccaattg gaaccccgcc ggcttcatgc gcaatgtcat cgaatcagtc
     8581 gatgtcatcg cggcctgaga ggactggccg tgctccggac attcattgcc gtgggggttc
     8641 tcctgggcgc tgcgctcgcg ccgttcgccg cctgcgccgc cgcacctctc gagttgaaat
     8701 cggtcacggt cgacctgccg gacagcgacc ggacattccc cccgggcccg ggatcggatg
     8761 cagtcaacaa caactgcctc gcctgtcatt ccgccggcat ggtgctcaac cagccggcat
     8821 tatcgaaggc cgcctggagc gccgaagtca acaaaatgat cacagcctac aaggcccccg
     8881 tggcgccaga agacgtcggt gcaatcgtcg aatacctgac cagcctccca agcgccaaat
     8941 agaaaaggga ccgggccaat ggtcgctcat ggcggcttca gcccccgaat gtcgtgccgg
     9001 agggcgatgg caggcagcgg ctgcagccac tcggcggcgc ccgcgcggtc gcgcagctct
     9061 cgctcgcgcc cgccgcgcac ggcgggatcg cggacaggaa gacgacgccc agcagccctt
     9121 ccggtgagag ctgcggacta ccgcctgaag cgagaccaag ggctccaacg ctcggactgc
     9181 cgaagttccg attcccatga tcatatgctc ctgcaatgtt ttgagcgatc acgatgtacg
     9241 tagagtaatg actgcggccg catcacagcc acgtaccgcc agcgatctct atggttgcct
     9301 cggctgcagc gcgcagtgcg ggcgctgcgc gcgcaccatc aagcggatca tggacgaagc
     9361 gctctcggct tccagctgtt gttgccgtaa aacgagcctg cctccggctc ccgtggttcg
     9421 tgccggagac ctacgcaccg ggagctcctc atggtccgca tcgcggcacg gtggaaatcg
     9481 ctcggcacgc aagtccggga gcacagggcc caactggggc tctccctcag ggtgacggtc
     9541 gccgctctgt tgagcttcgt actgtcacac ttgctgcatg ttcccctgcc attgtggacg
     9601 gtgctgacgg cggtgatcct gacgcaggtg agcttcggca agtcgctgaa ggcgaccatc
     9661 gactatctgg tgggtacgct cgggggcgcg gtttatgccg gcgccgtcgc cgcgctgttc
     9721 ccccacgcga acgaaatcgc gctggcaggc gtgctggcga ttgcggtcgc acccttggct
     9781 ctgctcggag cgatcaatcc ccgcttcagc gtcgcgacgt tcacgggggt gttggtgatc
     9841 ctggttcctg aaatcaccca tgtgagctcg atcgcatcgg cgttctatcg cgtgctcgag
     9901 gtcgcccttg gaggtctcac cgccgtagcg gtgtcattcc tggtgctgcc ggcgcgtgcc
     9961 catgttttgg cgatcgaggc cgcggcccag atgctcgatc tgatggcgcg gtctctgcct
    10021 gagttgttcg gtggcttcat gcaggcccgc gacgcaaccg cgatcgggcg catccaagac
    10081 aacatcggcc aagccttggc gcggttggag gccctcgtcg ccgaggcgag gcacgagcgg
    10141 ataagccttc tcgccgcgga gcccgatccg gggccactgc tgcgcacgct gctacgcctg
    10201 cgtcacgatc tggtgatgat cggacgggcg gctgccgttc cgcttcctga gccattccaa
    10261 ggacggttcg gaccgctgct ggcacagatc gcgaatagcg cagccgatta cctgcgccgg
    10321 agtggagaag cgctggccgc cgggcgcgat ccgccgtcgc tcgatgcggc cgaagcagcc
    10381 ctcgacgact acgccgatgc gttcaccatt gttcgccacg agggtcttac acgaggtctg
    10441 ccggccgaca cggcggagcg cattttcgcg ctgggttttg cccttgacca attgcaccag
    10501 aatttccgcg acctcgaacg ctgggtgagg gaagcggtag ctgggcggta aatgcggcca
    10561 cttcggcgca atttcgcgtt acacccggca aaatcgcgat agcgctattt tctcggcagc
    10621 atacatctcg gcagaactgg ccgccgcagc aggcgtcgac gctccttggg ccgggccggc
    10681 tattcaagat caggagttga ccatgagagt ccatcgtcca ttggctttcc tcccagtcat
    10741 attcctgctg acccagcctg cgtgggcggc accacgatct ggaggatccg caccgcatcc
    10801 ggcccttcgg gggctgagcg gcgttccggc cgaggtgcca aatcctatca caccacaatt
    10861 caacgatccg ggtccgcagc tcaccccggt acggccgggc aatccggtgc agcagctctc
    10921 gcccctcggc aattcggggc aaccggactc gctgggaatc cgataaagga cggaatttgt
    10981 tgaagacaga agtccaactg gttgggcggg cggggtcggc tccgcctact tgccgttgct
    11041 tgtcgcgatt tccggcggtc caatcggagg ctgcgcactg ttttttgggg agatcgaatc
    11101 ggccgggtga acggaggtga agcgcgactt ctcctcggca gcttggttgt tgccaagtga
    11161 tccactccgg ctttccgcat agaatccgga cctcgacagt caggtcaggg cagcgatgcg
    11221 cgaccaaacg gcacggctcg tgccggcgtc ggcacgccat cgcgccacca cagcatcgac
    11281 gcgccgatag tcggattgcc gcaagaagca ataagcacca gcaagatcgc gtaagcagcc
    11341 gcgacgctgc gtactacctg tcctttgcgg tccaccgatc ggatgttcgc acttgagacg
    11401 gccggtcgtg aaccgttcac accagtagtt gacgcgcggc tcgcgtccaa aggagaatgt
    11461 tatgttgcgc aagatgatga tggtcgcggc agcggttgcc gctctttccc tcgtcgccac
    11521 cgatggtttc gcgcgcggtg gaggcggggg cggtgggggc ggtggtggcc atg
//
LOCUS       DASWGM010001042         7257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2752170,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001042 DASWGM010000000
VERSION     DASWGM010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2752170"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VGA15_28020"
     CDS             <1..327
                     /locus_tag="VGA15_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9591578.1"
                     /translation="VESVKVEHLTVARSYGATAAQTAFRVYLPSMLPVLLEALRISMI
                     FNLTGVILAEMYASRDGIGHQIATWGENFQMKQLLAGVLMIGAIAIAFNELVRWVETR
                     CSHWRT"
     gene            306..1112
                     /locus_tag="VGA15_28025"
     CDS             306..1112
                     /locus_tag="VGA15_28025"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591579.1"
                     /translation="MQPLANVTPLKPAARAGSVQVTGVGQIYKTSGQDVVALSDVSIE
                     VKPGRFVVLVGPSGCGKSSLLMMMAGLRPQTSGTILINGAPIDVPDPDRVGVVFQEPS
                     LFPWLTAEENVEFPLTLRGVSKKERRVKAEEALQLVGLDGFGKRHPHELSGGMKQRVS
                     IARGLVQDPPVLLMDEPFAALDEQTRMTMGDELLRIWAATGKTIIFVTHSLTEAVYLA
                     DEVVVMSARPGRIVDRLDVALPRPRTFEMLSGDAFGSLRDRIWRHIRKSA"
     gene            1123..1929
                     /locus_tag="VGA15_28030"
     CDS             1123..1929
                     /locus_tag="VGA15_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9591580.1"
                     /translation="MSSTTLRRLIVIALIVLWEVLPRIGAIPELFLPSLSSTLQAGWS
                     DAGEYGHALVVTLYEVAVAMVFACGGGILGGAVVGSLPRPRVLIMPMVSSLYAVPLVI
                     LYPVFTVWLGIGSESKIAFASVYGFLPTMLATAAGIQTIDPQLLLAARSMGATLPQRI
                     TRVIIPAAIPTVLSGLRVGGALVIVGVVVSEMLISSAGIGYLISRYRTILESAHVFAG
                     VLLVLVMAVAFNALIKFVERRAAVWQTGTRAGRDTIAREIPAAALQPTNS"
     gene            complement(1949..2989)
                     /locus_tag="VGA15_28035"
     CDS             complement(1949..2989)
                     /locus_tag="VGA15_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HEX9591581.1"
                     /translation="MPLPKHVLPTTVVGSYPQPDWLVDREMLSKSVPRTRMTQMWRIP
                     EAYLEQAQDDATILAIRDLERAGIDIVTDGEIRRESYSNRFATALDGIDTDNPAIITS
                     RSGQKTPVPRVVGKVVRTGPIELRDMEFLRRNTDRAAKITLPGPFTMSQQAKNEFYKD
                     DEELAMALAAAVNAEALELQKAGADVIQLDEPWVRNDPAAAKRYAVKAINRALQGVTV
                     PTVVHICFGYAAVVPGDTKPSGYSFLAELADSTAQQISIEAVQPKLDLGVLADLSSKK
                     ILLGVIDLGNPEIETAAVIADRIRHGLNYVSADRLIPAPDCGMKYMPRHVAFGKLKAM
                     SEAAAIVRKEIS"
     gene            complement(3111..4082)
                     /locus_tag="VGA15_28040"
     CDS             complement(3111..4082)
                     /locus_tag="VGA15_28040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX9591582.1"
                     /translation="MQTRLRCLALAFAFALAAGIAWADDYPSRPIRLIVPYAAGGGAD
                     SVARIIAKRVSETIGQTIVIENRGGGGSIIGTDLVKNSDPDGYTLLLGQSGPISINPA
                     IYRKLPYDPVKDFAPISMTTAYPYIMVVNPGLGVKTLKDFVALAQSKPGELNYGTTGV
                     GAANHLVTELFDSKARIKMTHIPYRGTALAVTDLLAGQVQVVFADPVSALSHIKAGTL
                     LALAVTSKDRSPVAPDVPTVSESGYPGFDAIAWHGVLAPANTPPAIVNKLNAEIVTAL
                     KDPETRTLLEQQAMQIIGSSPEAFAGFIKRDIAVWKQVADQAKVEVR"
     gene            4240..5589
                     /locus_tag="VGA15_28045"
     CDS             4240..5589
                     /locus_tag="VGA15_28045"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX9591583.1"
                     /translation="MMLHKSGVTQSLARFIAATRWDDIPAPVRHQAKRSLMNFFAVAL
                     AGCRTGPVEIALRSLAEFSGGRQATLVGRAERIDALSAAFLNAAGANVHDFCDTHLRT
                     VIHPSAPVAPALLALAELRRVSGPDLLLAFILGNEIQARIGLAISPNHYSRGWHITST
                     CGVFGAAGGAGKLLRLDERRIVWALGTASTQSSGLCECLGTPAKSVGVGNAARNGLWS
                     ALLAEKGFDGPPEPLAGVQGFYNAMGETPDLSVVIDGLGESWEIMATSYKPYPCGFVI
                     HPVLDCVLDWRRDNPKADVTRVVVRGNPLLSDRTDRPDISTGRESQVSVQHAVAAALV
                     TGKAGLDQFTDACVRDPKVLALRGKVEVLRDSAFSTIAASVEITTADGTTHRLSQPAA
                     RGSDVNPMSDSDLEAKLRSAAAGWDPRYDVAPLVDAIWALDKSADVSRLASLAVPRD"
     gene            5705..7066
                     /locus_tag="VGA15_28050"
     CDS             5705..7066
                     /locus_tag="VGA15_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX9591584.1"
                     /translation="MRKSRIIGSVTAALVIAGAVAAFAIAWRPAIAAIDPPTPQSFDA
                     ALVKRGRDLAAIGNCNDCHTVRGGKNFAGGLPVPTPFGTIYSSNITPDAETGIGRWPE
                     AAFRRAMRSGVDRDGQHLYPTFPYDHFTNVTDEDDQALYAYLMTRQPVHAPARANQLA
                     FPLDQRFVIAGWKLLFLRRGTFQPDSAQSAEWNRGAYLVEGLAHCGSCHTPRNALGAE
                     RATAQFAGGDVDNWHAYAINAQSPAPVPWDADALFAYLRDGWHPDHGVARGPMAEVVS
                     NLSSVPPSDIRAIAIYMAGVFGAPTPDRKRRGEAVLAQVRSPSVPAPKADAVGAAIYA
                     AACATCHETGRTLPYGGVNLALSTAIAGPDPRNVANIVLSGVRPVEGERSPIMPGFAA
                     SMNDGQVTALLNYLRARFSSQPAWTNVEKTVEDARRTQIVFLQTSPGPLDAPAHPTQR
                     DKP"
     gene            7063..>7257
                     /locus_tag="VGA15_28055"
     CDS             7063..>7257
                     /locus_tag="VGA15_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX9591585.1"
                     /translation="MMTLKVNGIDHQIDADPDTPLLYVLRDDIKLNAAKFGCGLGQCG
                     SCTVIVDGKAVLSCVTPLLLL"
BASE COUNT         1210 a         2268 c         2375 g         1404 t
ORIGIN      
        1 gtcgaatccg tgaaggtcga gcatctgacg gtggcgcgct cttatggggc gacggcggcg
       61 cagacggcat ttcgggttta tctgccgagc atgttgccgg tgctgctgga ggcgctgcgg
      121 atttccatga tcttcaatct caccggcgtc attctcgcgg agatgtacgc ctcgcgcgat
      181 ggtatcggcc accagatcgc gacctgggga gaaaacttcc agatgaagca attgctggcc
      241 ggcgttctga tgatcggcgc gatcgccatt gccttcaacg aactcgtcag gtgggtggaa
      301 acgcgatgca gccattggcg aacgtaaccc cgctcaagcc ggcggcgcgc gcaggctccg
      361 ttcaggtcac cggtgtcggc cagatttaca agacctccgg ccaggatgtg gtggcgctgt
      421 ccgacgtatc gatcgaggtc aagcccggcc gttttgtcgt gctggtcggt ccaagcggct
      481 gcggaaagtc gtcgctgctg atgatgatgg ccgggttgcg gccgcaaacc tcggggacga
      541 tcttgatcaa cggcgcgccg atcgacgttc ccgacccgga ccgggtcggc gtggtgttcc
      601 aggagccgag cctgtttccg tggttgaccg ccgaagagaa cgtcgaattt ccgctgacgc
      661 tgcgcggcgt ttccaaaaaa gagcgccggg tcaaggcgga agaagccttg cagctcgtgg
      721 gtctcgacgg cttcggcaaa cgtcacccgc atgaactctc cggcggcatg aagcagcggg
      781 tctcgatcgc acgggggctg gtgcaggatc cgccggtact gctgatggac gagccgttcg
      841 ccgcactcga cgagcaaacc cgcatgacca tgggcgatga attgctgcgg atttgggcgg
      901 cgaccggcaa gaccatcatc ttcgtgaccc acagcctgac cgaagccgtc tatctcgcgg
      961 atgaggtggt ggtgatgtcg gcgcgtcccg gccgcatcgt ggaccggctg gacgtcgcgc
     1021 tgccgcgccc gcgcaccttt gagatgctga gcggcgacgc gttcggaagc ttgcgcgacc
     1081 gcatctggcg ccatatccgc aagtcggcgt aaggggatgg tgatgtcgtc gaccaccctg
     1141 cgccggctca tcgtcattgc cctcatcgta ttgtgggaag ttctgccgcg catcggcgcc
     1201 attcccgaac tgttcctgcc gtccttgtcg tcgacgctgc aggccggctg gagcgacgcc
     1261 ggcgagtacg gccacgcgct ggtggtgacg ctgtacgagg tcgccgtcgc catggtgttt
     1321 gcatgcggcg gcggaatctt aggcggcgcg gtggtcggca gcctgccgcg gcctcgcgtg
     1381 ctgatcatgc cgatggtgtc cagcctctac gcggttccgc tggtgattct ctatccggta
     1441 ttcacggtgt ggctcgggat aggctcggaa tccaagatcg ccttcgccag cgtgtacggc
     1501 tttctgccga ccatgctggc gaccgccgcc ggcattcaga ccatcgatcc gcaattgctg
     1561 ctggcggcgc gcagcatggg cgcgacgctc ccacagcgca tcacccgcgt catcattccg
     1621 gcagcgattc ccaccgtgct gtcaggcttg cgggtcggcg gcgcgctggt gatcgtcggc
     1681 gttgtcgtat cggagatgct gatctcgtcg gcgggcatcg gctatctgat ctcgcgctac
     1741 cggacgattt tggaaagcgc gcatgtgttc gccggcgtgc tgctggtgct ggtgatggcc
     1801 gtcgccttca acgccctgat caagttcgtg gaacgacgcg ctgcggtctg gcagactggt
     1861 acgcgcgcgg ggcgggatac gatcgcgcgc gaaattcccg ccgctgcgct gcagccgacg
     1921 aattcctgac ggcactgctc gtcaagtatc agctgatttc cttccgcacg atggccgcgg
     1981 cctcactcat cgccttcagc ttcccgaagg cgacgtggcg cggcatgtat ttcatgccgc
     2041 agtccggcgc cgggatcagt cgatccgcgg agacgtagtt gaggccatgc cggatccgat
     2101 cggcgatgac ggccgcggtc tcgatctcgg gattgccgag gtcgatcacg ccgagcagga
     2161 ttttcttgga cgatagatct gcgagcacgc cgagatcgag tttgggctgc acggcctcga
     2221 tcgagatttg ctgggcggtg gaatccgcaa gctccgccag aaaggaatag cccgacggct
     2281 tggtatcgcc gggcacgacc gcggcgtagc cgaagcagat gtggaccacc gtcggcaccg
     2341 tgacgccctg cagggcgcga ttgatggcct tcacggcgta gcgcttggcg gcggccggat
     2401 cgtttcgcac ccagggttcg tcgagctgga tcacgtcggc accggccttc tgcagctcca
     2461 gcgcttcggc attcaccgcc gccgccaatg ccatcgcgag ttcttcatcg tccttgtaga
     2521 attcgttctt ggcctgctgg ctcatggtga atgggccggg cagcgtgatt tttgctgcgc
     2581 gatcggtgtt gcggcgaagg aattccatgt cccgcagttc gatcggaccg gtacggacaa
     2641 ccttgcccac cacgcgcgga accggcgttt tctgtcccga ccgcgaggta atgatggcag
     2701 gattgtcggt gtcgattccg tccagcgccg tcgcaaaccg gttggaatag ctttcccgcc
     2761 tgatctcgcc gtcggtcacg atgtcaatgc cggcgcgctc caggtcgcgg atggcgagga
     2821 tcgtcgcatc gtcctgcgcc tgctcgagat acgcctcggg aatccgccac atctgcgtca
     2881 tccgggtccg cggaacggat tttgacagca tctcgcgatc caccagccaa tcgggctgcg
     2941 gataacttcc aacaacggtg gtcggcagca cgtgcttcgg tagcggcaat atctcaactc
     3001 cccggggcag acggattgtt cttataccga atgtacgggt tatctcccgt gaatgtaaac
     3061 gctctcgaat ttcggaagcg tccttgctgc ttcgcgggtc tcgaccgcac ttacctgact
     3121 tccaccttgg cctggtccgc cacttgcttc cagaccgcga tatcccgctt gatgaaaccg
     3181 gcgaaggcct caggcgaact gccgatgatc tgcatcgcct gctgttccag cagcgtcctg
     3241 gtttccggat ctttcagcgc cgtcacgatc tctgcgttga gcttgttgac gatggcaggc
     3301 ggcgtattgg ccggcgccag aacgccgtgc caggcgatgg cgtcgaaccc gggatagccg
     3361 ctctcggaga ccgtcggcac gtcgggggcg accggtgagc ggtccttgct ggttaccgcc
     3421 agcgcgagca gcgtacccgc cttgatgtgc gacagcgccg aaaccggatc ggcgaacacc
     3481 acctgcacct ggccggcgag cagatcggta accgccagcg cggtgccgcg ataggggata
     3541 tgcgtcatct tgattctggc tttgctgtcg aacaattcgg tgaccagatg attggcggcg
     3601 ccgacacctg tggtgccgta gttcaactcg cccggcttgc tttgggccag cgcgacgaag
     3661 tctttcaggg tcttcacgcc cagtccgggg ttgacgacca tgatgtaggg ataggctgtt
     3721 gtcatcgaga tcggcgcaaa gtccttcacg gggtcgtagg gaagctttct gtagatcgcc
     3781 ggattgatcg agatcggccc ggattggccg agcagcagcg tatagccgtc agggtcggag
     3841 ttcttcacga gatcggtgcc gatgatcgat ccgccgccgc cgcggttttc gatgacgatg
     3901 gtctgtccaa tggtctcgct gacgcgtttg gcgatgatgc gcgcaaccga gtcggcaccg
     3961 ccaccggcag catagggtac gatgagccgg attgggcgcg acggataatc gtctgcccac
     4021 gcgatgcccg cggccaaagc gaacgcaaag gcgagcgcca ggcaccgtaa tctcgtttgc
     4081 accgtgttcg ctccccaagt caattattcg gaccgcgctg gcggcggcct tctgatcgcc
     4141 cgcagcagca tagtgcaaat tttcctgccc ggtctaccga acgctctgtt gcgctttggc
     4201 gcgggccagc caatgcttgc gccgattttg gggaggacaa tgatgctgca caagtcaggc
     4261 gtgacgcaga gcctcgcccg ctttattgcg gcaacgcggt gggacgacat accagcgccg
     4321 gtgcggcatc aggccaaacg ctcgttgatg aatttctttg ccgtggcgct cgcgggctgc
     4381 cgcaccgggc cggtcgagat cgcgctgcgc tcgctcgccg aattctccgg cggcagacag
     4441 gcaaccctgg tcgggcgcgc cgagcgcatc gatgcgctga gcgcggcctt cctcaacgcc
     4501 gccggcgcca atgtccatga cttctgcgac acccatcttc gcaccgtgat ccacccctca
     4561 gcgccggtgg cgccggcgct gctggcgctc gccgaattgc ggcgggtgag cgggcccgat
     4621 ctgctgctcg ccttcatcct cggcaacgag attcaggcgc gcatcggtct ggcgatctcg
     4681 cccaaccact acagcagggg ctggcacatc acttcgacct gcggcgtatt cggcgccgct
     4741 ggtggggcgg gcaagctgct cagattagac gaacgccgga tcgtgtgggc gctcggcact
     4801 gcgtccacgc agtcgtccgg cttgtgcgag tgcctgggca cgccggcgaa aagcgttggc
     4861 gtcggcaacg cggcgcgcaa cgggctgtgg tcggcgctgt tggccgagaa aggttttgat
     4921 gggccgccgg agccgctcgc gggcgtgcag ggtttctaca acgccatggg cgagacgccc
     4981 gacctttcag tggtcatcga cggcctcggc gaaagctggg agatcatggc gacgtcatac
     5041 aagccgtatc cgtgcggctt tgtcattcat ccggtgctcg attgcgtgct ggactggcgg
     5101 cgcgacaatc cgaaagccga cgtcacgcgc gtggtggtgc gcggcaaccc gctgctgagc
     5161 gaccgcaccg accggcctga tatttcgacc gggcgggagt cgcaggtcag tgtgcagcac
     5221 gcggtcgccg ccgccctggt gaccggcaag gccgggctcg accagttcac cgacgcctgc
     5281 gtgcgtgatc cgaaagtgct ggcgctgcgc ggcaaggtcg aagtgctgcg ggattccgcg
     5341 ttctccacta tcgcggcatc ggtcgagatt accaccgccg acggcacgac ccaccgactg
     5401 tcgcagccgg cggcgcgcgg cagcgacgtc aatccgatga gcgacagcga tctcgaggcg
     5461 aagttgcgca gcgccgccgc cggctgggat ccgcgctacg acgtagcgcc gttggtcgat
     5521 gcgatctggg cactcgacaa aagcgccgat gtttccaggt tggcttcgct ggccgtgccc
     5581 cgggactaag ctgacaagag gaaccctcaa caaaccggaa gtcaacagac ggtgatttgg
     5641 gatcccgcgg aaaactccgg gcgcggattt gtgttaacaa tggccgcaaa agccgtaggg
     5701 acggatgaga aaatcgcgaa ttatcggtag cgttaccgcg gcgctcgtga tcgcgggcgc
     5761 ggttgcggcc tttgcgatcg cgtggcggcc ggcgatcgct gcgatcgatc cgcccacgcc
     5821 gcagtctttc gacgcagccc tggtcaagcg aggccgcgat ctggccgcga tcggcaattg
     5881 caacgattgc cataccgtgc gcggcggcaa gaactttgcc ggcggccttc cggtgccgac
     5941 gccgttcggc acgatctatt cgtcgaatat cacaccggat gccgagacgg ggatcggccg
     6001 gtggccggaa gccgcctttc gtcgtgccat gcggtcggga gtcgatcgcg acgggcagca
     6061 cctctatccg acatttccct acgatcactt taccaatgtg accgacgaag atgaccaggc
     6121 gctctacgct tacctgatga cacggcagcc ggttcacgcg ccggcccgcg cgaaccagct
     6181 tgcctttccg ctcgaccagc ggtttgtgat cgccggctgg aagctgctgt tccttcgtcg
     6241 cggcacgttt cagccggact cagcgcagag cgcggaatgg aatcgcggcg cctatctggt
     6301 cgaagggctg gcgcattgcg gttcatgtca caccccgcgc aatgcgcttg gcgcggaacg
     6361 tgcgaccgcg cagtttgccg gtggcgatgt cgacaattgg cacgcttatg cgatcaacgc
     6421 gcaatctccg gcgccggtgc catgggacgc cgacgcgctg ttcgcctatc tccgcgatgg
     6481 ctggcatccc gatcacggcg tcgcgcgcgg gccgatggct gaggtggtca gcaatctgtc
     6541 atcggtaccg ccaagcgata tccgggccat tgcgatctat atggccggcg tgttcggcgc
     6601 gccgacgccg gatcgcaaac gtcggggcga agcagtgctg gcacaagtca ggtcaccctc
     6661 ggtgccggcc ccaaaggccg acgcggtcgg cgcggcgatc tatgccgcgg cctgcgcgac
     6721 atgccatgag accggcagga cgttgcccta tggcggggtc aatctcgctc tttctactgc
     6781 catcgctggc cccgacccgc gcaacgtcgc caacatcgtg ctgtcgggcg ttcgacccgt
     6841 ggaaggagag cgcagcccga tcatgccggg tttcgcggcc agcatgaatg acggccaggt
     6901 tacagcgctg ttgaactatt tgcgggcgcg gttcagcagc caaccggcat ggaccaacgt
     6961 tgaaaagacc gttgaggacg cgaggcgcac ccagatcgtg tttcttcaga catcgcccgg
     7021 gcccctcgac gcgcccgccc atccaacgca gcgagacaag ccatgatgac attgaaggtc
     7081 aatggtatcg atcaccagat cgatgccgat ccggacacgc cgctgcttta tgtcctgcgc
     7141 gacgatatca agctcaatgc cgccaagttc ggctgcgggc tcggccaatg cggttcgtgc
     7201 acggtgatcg tcgacggcaa ggcggtgttg tcgtgcgtga cgccgctgct tctgctc
//
LOCUS       DASWGM010001043         2898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1365968,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001043 DASWGM010000000
VERSION     DASWGM010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365968"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VGA15_28060"
     CDS             complement(<1..550)
                     /locus_tag="VGA15_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX9591586.1"
                     /translation="MLELVIISTLNGVLYGMLLFLMASGLTLIFSMMGVLNFAHASIY
                     MIGAYLAFTLSQLLGFFPALIIAPVLCGLIGAAIEVWGLRRVHNNGHIAELLFTFGMV
                     FIIQRGVQMIWGMLPMAYRVPPLLDFALFTVYGANFPAYRGFMLLVSVGMLVATWLLL
                     KKTRVGLIIQAALTHPQMASALG"
     gene            complement(840..2084)
                     /locus_tag="VGA15_28065"
     CDS             complement(840..2084)
                     /locus_tag="VGA15_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9591587.1"
                     /translation="MNFVHLKAALLAGAAVCLSSPAGAQETVRIAFIDPLSGLAASTG
                     EAGLKTFQYLAEQVNAKGGVLGKKLEVVGYDNKVNAQESLVQLQKAIDAGIRIVTQGN
                     GSNVAAALEDQVGKYNDRNPGKEVVYFNYAAVDPVLTNEKCNFWHFRWDANSDIKMEA
                     LTNYMKTRTNIKKIYLINQDYSFGQAVRSAARKMLSEKRPDIQLVGDEVHPLQKITDF
                     SPYVAKIKASGADSVVTGNWANDLSLLIKAAADAGLQVDWYTYYAGGPGGPTAIKQAN
                     VPDRVFQITEGYPNIPYKPSQDFEKAFRAKIGQSLFYPRAVNEMNMLVKAIQEANSTE
                     PGKIAAKLEGMKVEVHNGGEGFMRADDHQFFQPIYIANLGPIGKDQPFDEEGTGWGWK
                     VTGKVDTNNTIVPTSCKMNRPS"
     gene            2281..2739
                     /locus_tag="VGA15_28070"
     CDS             2281..2739
                     /locus_tag="VGA15_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX9591588.1"
                     /translation="MRVFADFDAVKAAVGEDIGVSDWVLVSQQRIDRFAEATGDDQWI
                     HVDVERARRELPYGTTIAHGLLTLALVPSFFRTVLRVERVKNSLNYGSNRVRYLSPVP
                     AGSRLRGRIGIAQAVDVPPNGLRVTYHTTVEIEGSERPACVADTIGVHFR"
BASE COUNT          505 a          914 c          920 g          559 t
ORIGIN      
        1 ggccgagcgc gctcgccatt tgcggatggg tcaaggccgc ctggatgatc aggccgacgc
       61 gggttttttt caacagcagc catgtcgcca ccagcatgcc gacggaaacc agcagcatga
      121 agccgcgata ggctggaaag ttggcgccat agacggtgaa caacgcaaag tccagcagcg
      181 gcggcacccg ataggccatc ggcagcatgc cccagatcat ctgcacgccg cgctggatga
      241 tgaacaccat gccgaaggtg aaaagcaatt cggcgatgtg gccattgtta tggacccgcc
      301 tgaggcccca gacctcgatg gcggcgccga tcaggccgca cagaaccggc gcgatgatga
      361 gggcgggaaa gaagccgagc agctggctca gcgtgaacgc cagatacgcg ccgatcatgt
      421 agatgctggc atgggcgaaa ttcagcacgc ccatcatgct gaagatcagc gtcaagccgc
      481 tcgccatcag gaacagcagc atgccgtaga gcacgccgtt gagggtcgag atgatgacaa
      541 gctcgagcat ttcttcagcg ctccgagcac gccacatcct gctccctccc cccttgtggg
      601 ggagggatgg ggaggggggt atttgtttga tactgccggc cagacacccc cctcccggct
      661 cgcgcgccgg acgcgcgcgg agccgacctc ccccacaagg ggggaggtaa ccggagcgag
      721 ctgatcgtca tatgggacag cgtgcccatg ggagaggtca ccggagtgcg tccgccgtca
      781 tcatgatccc cctgccgacc ttgtgtcggt cgcagttcca cggcggaaat tcaccgacgt
      841 caactcgggc gattcatctt gcagctggtc ggcacgatgg tgttgttggt gtccaccttg
      901 ccggtgactt tccagcccca gccggtgcct tcctcgtcga acggctggtc cttgccgatc
      961 ggcccgaggt tggcgatgta gatcggctgg aagaactgat ggtcgtcggc ccgcatgaag
     1021 ccttcgccgc cattgtgcac ctcgaccttc atgccctcga gcttggcggc gatcttcccc
     1081 ggctcggtcg aattggcttc ctggatggct tttaccagca tgttcatctc gttgacagcg
     1141 cgcggataga acagcgactg gccaatcttg gcccggaacg ccttctcgaa atcctgcgac
     1201 ggcttgtacg gaatgttcgg atacccttcg gtgatctgga atacccggtc gggtacattg
     1261 gcctgcttga tcgcggtcgg gccgccgggg ccgccggcgt aataggtgta ccaatcgacc
     1321 tgcaggccgg cgtcggctgc ggccttgatc agcagcgaca gatcgttggc ccagttgccg
     1381 gtcacgacgc tgtcggcgcc ggacgccttg atcttggcga catacggcga gaagtcggtg
     1441 atcttctgca gcggatgcac ctcgtcgccc acgagctgga tgtcgggccg cttctccgag
     1501 agcatcttgc gggccgcgct gcgcaccgcc tggccgaacg aataatcctg gttgatcaga
     1561 tagattttct tgatgttggt gcgcgtcttc atgtagttgg tgagcgcctc catcttgatg
     1621 tcggaattgg cgtcccagcg gaaatgccag aaattgcatt tctcgttggt gagcaccggg
     1681 tcgacggcgg cgtaattgaa atacacgact tccttgcccg gattgcgatc gttgtatttg
     1741 ccgacctggt cctcgagcgc ggcggcgacg ttcgagccgt tgccctgggt gacgatgcga
     1801 atgccggcgt cgatggcctt ttgcagctgc accaggcttt cctgggcgtt gaccttgttg
     1861 tcgtagccca cgacttcgag cttcttgccg aggacgccgc ccttggcgtt gacctgctcg
     1921 gcgagatact ggaaggtctt gagccccgcc tcgccggtgc tcgcggcaag cccggacaac
     1981 ggatcgatga aggcgatccg aacggtttcc tgggcgcccg cgggagaact caggcacacc
     2041 gccgcgccgg ccagcaacgc cgctttcaaa tgcacgaaat tcattgcact cctcccgttt
     2101 gatcgatcct tgttgcgcaa tctcatgcgc ggccgacgcc tagatgatcc ccgggggcgt
     2161 gcgtagtcaa gcgcccgccc gggccggatg cggcgaacca tcacctgcct gcacggggct
     2221 cgcttcctcg ccggcttttg gcgtaaatcc cagcccggcc attggaccac ggagggattc
     2281 atgcgggtgt ttgccgattt cgacgccgtg aaagccgccg tcggcgagga catcggggtc
     2341 agcgattggg tgctggtctc gcagcagcgc atcgaccgct tcgccgaggc gaccggcgac
     2401 gaccaatgga tccacgtcga cgtcgagcgg gcccgtcgcg agctgcccta cggcacgacc
     2461 atcgcgcacg ggctgctgac gctggcgctc gtgccgtcgt tctttcgcac cgtgctccgc
     2521 gtcgagcgcg tgaagaacag cctcaattac ggctcgaacc gggtgcgcta tctcagcccg
     2581 gttccggccg gctcgcggct gcgcggccgc atcggcatcg cgcaggcggt ggatgtcccg
     2641 cccaacggcc tgcgcgtcac ctaccacacc acggtcgaga tcgaaggcag cgagcggccg
     2701 gcctgcgtgg ccgacaccat cggggtgcat ttcaggtgat cagaagtcag aaatcagaaa
     2761 tcacggcccg atcaccctac cgacttgtga ttctgatcac tgtctcctgg acggcggctg
     2821 gtatttgcgc agctcgagct tcgccaccgt ctcgcgatgc acttcgtccg ggccgtcggc
     2881 gaggcgcagc gtgcgcgc
//
LOCUS       DASWGM010001044         8004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1103453,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001044 DASWGM010000000
VERSION     DASWGM010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103453"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGA15_28075"
     CDS             complement(<1..369)
                     /locus_tag="VGA15_28075"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX9591589.1"
                     /translation="MSQQFASRPLVIAPSILASDFARLGEEVRAVDQAGADWIHLDVM
                     DGHFVPNISYGPDVIKAMRPHTKKIFDAHLMISPCDPYLEAFAKAGCDHITVHAEAGP
                     HLHRSLQAIRALGKKAGVSLN"
     gene            1177..1986
                     /locus_tag="VGA15_28080"
     CDS             1177..1986
                     /locus_tag="VGA15_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX9591590.1"
                     /translation="MLLALGAASSVIDAFKSLTQTKAKSSSPQATGFTQSSTNPFDAS
                     SSTSTSASPAPASGSTGVSQISPSTMSALLAAQSQSSTATTTSTSTSPSSALQDLFSQ
                     IDANGDGQISQSEFESALGAGGTNTAQADDVFSKMDKNGDGSVSLDEMSSALKGGGHH
                     HHHHVAGSDGSSGATGDSSSTSGSSSDPLLQALDGATSTSTTNSDGSTTTSMTYADGS
                     KVTMTMPATKSSSSATSSSSATSSYNIIEQMIQRQAQAISSGATSSLSMSV"
     gene            complement(2024..3019)
                     /locus_tag="VGA15_28085"
     CDS             complement(2024..3019)
                     /locus_tag="VGA15_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX9591591.1"
                     /translation="MKNLLTDIAGVRVGHADDAALASGVTAVMFDKPAVAAIDARGGG
                     PGTREGALLDLSNTVQRIDAIALAGGSAFGLEAGGGVQAWLAEQGRGFAVRGAIIPIV
                     PGAIMFDLLNGGNKAWGRFAPYRDLGYTAAAAASTNFAQGSVGAGLGATTANFKGGIG
                     SASALTQSGVQVAALAVVNAVGSVTVGDGPWFWAAPFEIDGEYGGRGLPPSFTPDMRT
                     MRIKGGAAATSVENTTLAVVVTDADLTNPQAKRLAMIAQTGFARAIYPVHAPLDGDVV
                     FAAATGEKPIDPLVGLTELGMVAANVVARAIARGVYAATALPFPGALPAWKNRFG"
     gene            complement(3204..4001)
                     /locus_tag="VGA15_28090"
     CDS             complement(3204..4001)
                     /locus_tag="VGA15_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591592.1"
                     /translation="MLASRQGTKLKFPKWPVSGPVIALCLTASSGPAWAYRPFDGTDA
                     AIAAPGEMEIELQPAGRLHEGGNTSLVAPATVINYGLSEGWEAVLAGQGETALSPSGP
                     TSLTSAGAFLKHVLQPGSLQDKTGPSIATEFGMLLPDSTGSSGVGASLAGIVSQRWDG
                     GTIHLNAATALTRDHHGDLFLGGIIEGPSKWSIRPVAEFFYENEFGKSETISGLIGLI
                     WRVRDNLSFDVGLRHALTNGHQVNEVRAGLTFGFPLGQFGGRSAQSR"
     gene            complement(4032..5045)
                     /locus_tag="VGA15_28095"
     CDS             complement(4032..5045)
                     /locus_tag="VGA15_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591593.1"
                     /translation="MLRPLRTIVMVLAGVMALASTAGQAPAQSADTGLLQLETKILLG
                     DVRGRIDHMAVDLKRQRLFVAELGNDSVGIVDLPNRKLISRIPELKEPQGVGYEPTTD
                     MLYVANAGDGSVRLFEGSDYKTTGRIDLGSDADNIRIDAAAKRVFIGYGSGALAVIDP
                     STRSKVGDISLKAHPEAFQIDPDTSQIFVNVPGARGIAVVDRVSQKQIGEWPLADRGA
                     NFPMALDHVRRQVLVIFRAPAALGVFSMTDGKRIATAETCGDADDLFIDAKRGRVYVS
                     CGDGFLDVLEAREATYRRIARIPTVSGARTSLFVPEMDRLVVAVRANSGEPAAIWMFR
                     PMP"
     gene            complement(5111..5731)
                     /locus_tag="VGA15_28100"
     CDS             complement(5111..5731)
                     /locus_tag="VGA15_28100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591594.1"
                     /translation="MPHLGDGLLAEAKEKWGDINPPMEVNMTRRKLLLMTAGATVLGL
                     AALVGFADHNAIFAGDDEKEGQEALIKTLGAAKISLQQGLAASAQEGQPISAKFEVDE
                     GKLQLSVYTAKDGKFSELLVDYVNGKVTKAVPITEGDDLGAAKSQSAALATAKISLKE
                     AVDKAAAQSASARVVSAVPSLKVGHPVASIVLLDGERFKTVQQPLD"
     gene            complement(5831..6019)
                     /locus_tag="VGA15_28105"
     CDS             complement(5831..6019)
                     /locus_tag="VGA15_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591595.1"
                     /translation="MRIFALALLVLVAFYGANRTAQAQQSSCKVCGEQRQACMKNYAG
                     PTCKSEYQMCMKSCGKKS"
     gene            complement(6055..6999)
                     /locus_tag="VGA15_28110"
     CDS             complement(6055..6999)
                     /locus_tag="VGA15_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEX9591596.1"
                     /translation="MRKMLWITVSIFAASFAGGANAQKIDWQIFLEPFVTSAHAQEVD
                     WQKVDDAFGRKPAVSGDVHRYGFPRTDLTVALDGVTIRPALALGGWIAFKPMHGEAMV
                     MGDLVLLETEVNPVMLKLIEGGLEITAVHNHLLRASPATFYLHVGGHGDPAKMAAVIR
                     DALGASKTPLATPATAGPAPAVDLDTAQLDQIIGVKGQANGGVYQFAVPRRDPVTENG
                     MQISPVGPMGVANAIGFQPTGDGKAAITGDFVLTGDEVNPVIKALRANGIDVTAVHSH
                     MLDEQPRLFFLHFWANDDALKLAKGLRAALDKTASTKS"
     gene            complement(7060..7440)
                     /locus_tag="VGA15_28115"
     CDS             complement(7060..7440)
                     /locus_tag="VGA15_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591597.1"
                     /translation="MNRRTPQIVALTILMPTTICGPIAHAREMFKLLGEKENRTRVVG
                     KDITDSSHWVSYLRPDGVLLSSEMGRKWTGTWKIQNNKLCLSNPNLESPDCNEVWMSG
                     ANIRMRANKDQETFDATVEKHRPN"
     gene            complement(7437..>8004)
                     /locus_tag="VGA15_28120"
     CDS             complement(7437..>8004)
                     /locus_tag="VGA15_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX9591598.1"
                     /translation="GCDPVRIKGMSERLIISHYENNYGGAVKRLNLIGEKLAELDYAN
                     APGFLINGLKREELVAMNSMILHEHFFDSLGDQSEPDATLQEALARDFGSFERWRAEF
                     TAMGKALGGGSGWVLLSWSPRDRKLVNQWAADHCHTLAGGTPILALDMYEHSYHIDFG
                     AKAADYVGTFMAAINWPAVQRAYDGVRS"
BASE COUNT         1557 a         2485 c         2331 g         1631 t
ORIGIN      
        1 attgagcgag acgccggctt tcttgccaag cgcccggatc gcctgcagcg agcgatgcag
       61 atgcgggccg gcctcggcat gcaccgtgat gtgatcgcag ccggccttgg cgaaggcctc
      121 cagatacgga tcgcacggcg aaatcatcag atgcgcatcg aagatttttt tggtgtgcgg
      181 gcgcatcgcc ttgatgacgt ccgggccgta ggaaatgttg gggacgaaat gcccgtccat
      241 cacgtcgagg tggatccagt cggcgccggc ctggtccacg gcgcggacct cttcgcccag
      301 tctggcgaaa tcggacgcca ggatcgacgg cgcgatgacg agggggcgtg acgcaaactg
      361 ttgagacatg tgcgctttcc cgaaccattt tcgaagcgtg gagcagtttt cgggtaacat
      421 gcgcaaagct cgggggcaat gcagggaaaa ctccacctgt gtctcggacg cggcgcgcgc
      481 tcccggcgat gcgaagctcg tccggcgcgg gcgctcgggc ttgggtccaa gtaacgttgt
      541 ttcagcatac ccccgcagtt tccccgcccg cgatagctcg cctcggttcc gatcccagtt
      601 tttctggctt gcaaattgtc atgggatgcc cttggcacga tattcgcatc gtcactgacg
      661 ctggcggtca ttggcggaat atttcctccg agcttcctgc ctcggtgagc aaaatgggcg
      721 aagtcgtgca attcatcccg aagcctgccc ccgaccgggc agcccgtctc attcaagagg
      781 cccgcgcgat atacgagagc atattcccga cggagacccc cagtatcgcg ccaatcgaag
      841 atgacgatac ttcgtcctga ttttttccct cggaaactgt tccttcaatc ttgatttggt
      901 ggaccgcctc gttttggcgg cgctatttgt tccttgtatg ccgcggggaa cgcgagcacc
      961 gccacgcgga gcgcggaccc cagcgcatta agcagcatcg ggggcacgtc gatgggcgga
     1021 aaattctgcc gcggcggcat caattgccgg ggagggtgga ccgttcggga tacaaaaacc
     1081 catattttac tgggtttttc gcggtggcac ggcgtttgct gatcaatcgg tgtcgaagta
     1141 acagccgcac gatgcggcat ttttcggagt tccgctatgc tgctcgccct tggcgccgcg
     1201 tcgtccgtaa tcgacgcctt caagtcgctg acccaaacga aagcgaaatc gtcgtctccg
     1261 caagccaccg gctttaccca gagctcgacg aacccgttcg atgcttccag cagtacctcg
     1321 acttcggcca gcccggcccc ggcgtccggg tccaccgggg tttcgcagat atcgccgtcg
     1381 acgatgagcg cgctgctggc ggcgcaaagc cagtcatcga ccgcgacaac gacgtcgaca
     1441 tcgaccagcc cgtccagcgc cctgcaagac ctgttctcgc agatcgacgc caacggcgac
     1501 ggccagatca gccaatccga atttgaaagc gccctcggcg ccggcggtac caacaccgcg
     1561 caggccgacg acgtgttcag caaaatggac aagaatggcg acggttcggt cagtctcgac
     1621 gaaatgtcgt cggcgctgaa gggaggcggt caccatcacc atcatcacgt cgccggttcg
     1681 gatggatcga gcggcgctac cggtgattcc agcagcacca gcggatcgag ctccgatccc
     1741 ctgctgcagg cattggacgg cgcaaccagc acgtcgacaa ccaacagcga cggctcgacc
     1801 accacatcaa tgacctatgc ggatgggtcc aaggtgacga tgaccatgcc agcgacgaag
     1861 tcgtccagct ccgccacctc atccagcagc gcgacctcat cctacaatat catcgagcag
     1921 atgattcagc gtcaggcaca ggcgatttca tccggagcga cctcatcgct ctcgatgagc
     1981 gtctgaaaca gcttggcgaa ccgtctgtta ggagcacctt gacctagcca aagcggttct
     2041 tccacgccgg cagcgcgccg gggaagggca gcgcggtcgc cgcgtaaacg ccgcgcgcga
     2101 tggcgcgggc cacgacattg gccgcgacca tgccgagctc ggtgaggccg accagcggat
     2161 cgatcggctt ttcgccggtc gcggcggcga acaccacatc gccatcgagc ggtgcgtgca
     2221 ccggatagat cgcgcgggca aatccggtct gcgcgatcat cgccagccgc ttcgcctggg
     2281 ggttggtcag atcggcgtcg gtgacgacca ccgccagcgt ggtgttttcc accgatgtcg
     2341 cggccgcgcc acctttgatg cgcatcgtgc gcatgtccgg cgtgaatgat ggcggcaatc
     2401 cgcgtccgcc atattcgccg tcgatctcaa atggcgccgc ccagaaccac ggtccatcgc
     2461 cgaccgtgac gctgcccacc gcattgacga ccgcaagcgc tgcgacctgg acgccgcttt
     2521 gtgttagcgc cgatgccgag ccgatcccgc ccttgaaatt cgcggtggtc gcgccgagac
     2581 cggcgccgac gctcccctgt gcgaaattgg tgctggccgc ggcggcagcg gtgtagccga
     2641 gatcgcggta gggcgcgaag cggccccagg ccttgttgcc gccgttgagc agatcgaaca
     2701 tgatcgcgcc cggcacgatc ggaatgatcg cgccgcgcac cgcaaagccg cggccctgtt
     2761 cagcgagcca ggcctgcacc ccgccgccgg cctcgagccc gaatgccgag ccgccggcga
     2821 gcgcgatggc gtcgatccgc tgcacggtgt tggataggtc aagcagcgcg ccttcgcggg
     2881 tgccgggacc gccgccgcgc gcgtcgatcg cggccaccgc cggtttgtcg aacatgaccg
     2941 ccgtgacgcc ggacgccagc gccgcatcat cggcgtggcc gacgcggacg cccgcgatat
     3001 cggtgaggag gtttttcaaa ggagaggacc ggctcccttt ccctcactcc ttacgttctt
     3061 gaggaggggt cgtggacgat ggaagttgtt ctccagaggg ggataccgcg ttgatatcgc
     3121 gtgcgtcaac agggagggac ggtcacgttt gatctaatca ggacgccttt tacatgcagg
     3181 cgctttgatt tcgaacgtta agattatctg gattgggccg agcgcccacc gaactgcccc
     3241 aggggaaagc cgaacgtcag ccctgcacgc acttcattta cctgatgacc attcgtgaga
     3301 gcatgacgca gcccgacgtc gaaactgaga ttgtcgcgca cccgccagat gaggcctatc
     3361 aaacccgata tcgtttcaga tttgccaaac tcgttttcgt agaaaaactc tgcaacgggc
     3421 cggatcgacc actttgatgg cccctcgatg atgccgccca gaaacagatc gccgtgatga
     3481 tcgcgcgtga gcgcggtcgc ggcgttcaag tggatcgtcc ccccgtccca gcgctgcgag
     3541 acgattccag ccaaacttgc accgacgccc gaactgccgg tgctgtcggg aagcagcatg
     3601 ccaaattccg tcgcaatact cggtccggtc ttatcctgca gactgccggg ctggaggaca
     3661 tgtttgagga atgcacccgc agacgtcagg ctggtgggac cagagggtga aagagcagtc
     3721 tcgccctggc ccgcaagcac ggcttcccat ccctcgctca atccgtaatt aatgaccgtt
     3781 gccggggcga ccagactggt atttccacct tcatgcaggc ggccggctgg ttgcaattca
     3841 atttccatct ctcccggcgc tgcgatcgcc gcatcggttc cgtcgaatgg gcgataagcc
     3901 caggcaggac cggacgatgc ggtcaggcac aaggcgatga ctggtcccga cacgggccat
     3961 ttcggaaatt tcagtttcgt accctgccgg ctcgcaagca tgcgctggtc ctcagtgccg
     4021 cggctacttt ctcaaggcat tggtcgaaac atccagatgg ctgctggctc tcccgagttc
     4081 gcccggacag caacaaccag acgatccatc tcgggtacaa agagcgaagt gcgcgcaccc
     4141 gaaaccgtgg ggatacgcgc gatccgtcga taagtcgctt ctcttgcttc caaaacatca
     4201 agaaagccat cgccacaact cacgtaaacg cggccgcgct tggcatcgat gaacaggtca
     4261 tcggcatcac cgcaggtttc ggcggtcgcg atccgcttgc cgtccgtcat ggagaagacg
     4321 ccgagcgcgg cgggagcccg aaagatcacg aggacctggc gccgaacgtg gtccagggcc
     4381 atcggaaaat ttgcaccccg atcggccagc ggccattctc cgatttgctt ttgcgagacc
     4441 cggtccacga ccgcgatgcc gcgggcacct gggacgttga cgaagatttg gctggtgtcc
     4501 gggtcaatct ggaatgcttc ggggtgcgcc tttagtgaaa tgtccccgac cttgctgcga
     4561 gttgaaggat caatcacggc cagcgctccg ctgccatatc caatgaagac gcgcttggcg
     4621 gcggcatcaa tacgaatatt gtcggcatcg ctgccgagat cgattcgccc cgtcgtcttg
     4681 taatcgctcc cttcaaacaa tcgcaccgag ccgtcgccgg cattggcgac ataaagcata
     4741 tcggtcgtgg gctcgtagcc gacgccttga ggctccttca gttctggaat cctgctgatc
     4801 aattttcgat ttggcagatc gacaattccg acggaatcat tgcccaattc ggcgacgaac
     4861 agtcgttgcc gcttcaagtc aacggccatg tgatcgatgc ggccgcggac gtcgcccaac
     4921 agaatcttgg tttcgagttg gagcaggcct gtatccgcgg actgagcagg cgcttggcct
     4981 gcggtgctag ccagcgccat gacgccggct aacaccatta cgatcgtccg gagtggacgt
     5041 aacatggccg caccatcctt gacggacgaa aaaaccgctc gtccgatgcg ttgtttcagt
     5101 ggggacgtcc ctaatccaaa ggctgttgga ctgttttgaa ccgttcacca tcgaggagaa
     5161 ctattgaagc aacgggatgc ccaactttca gactgggcac cgcactgacc acgcgagcgc
     5221 tggctgactg ggccgcggcc ttatcgacgg cttccttcaa agagatcttt gccgtcgcca
     5281 gcgccgcgct ctgtgatttg gccgcaccga ggtcgtcccc ctcggtgatt ggtacggcct
     5341 tcgtcacctt gccgttcaca tagtcgacca gcagctcgga gaacttgccg tccttcgccg
     5401 tgtaaaccga cagctggagc ttgccctcat cgacctcgaa tttggccgag atcggctggc
     5461 cctcctgagc gctggccgcc aatccttgct gcaagctgat cttggcagcg ccaagcgtct
     5521 tgatcagcgc ctcctgacct tccttttcat cgtcgcccgc aaaaatcgcg ttgtggtcgg
     5581 cgaagcctac caaagcggct agtccaagaa ccgtcgcgcc ggcagtcatc agcaacaact
     5641 ttcttcgggt catgttgacc tccatcggtg ggttaatgtc gccccatttt tcttttgctt
     5701 cagctagtag accatcaccc aggtgcggca agcgcgctga tctcgaccgc gattgcagcc
     5761 actgtcgccg cggcaactat cctcaatacc tttcttcatg gcggcgtcta atcttgaaga
     5821 ggtccgcgtt tcacgatttc ttcccgcaag atttcatgca catttgatat tcagacttgc
     5881 acgtcgggcc ggcataattt ttcatgcagg cctgtcgctg ctcgccgcac actttgcagc
     5941 ttgattgttg agcctgtgct gtacgattgg cgccataaaa ggcaacaagc accagcagcg
     6001 ccaacgcaaa gattcgcatg ataagatttc ctcttggtgc tgagacagta gagtctaact
     6061 cttcgtgctc gctgtcttgt cgagcgcggc gcgcaatccc ttggcaagct tcaatgcatc
     6121 gtcattggcc cagaaatgga ggaagaagag tcgcggctgc tcgtccagca tgtggctatg
     6181 gacagccgtc acgtcgatgc cattcgctcg caacgccttg atcacgggat tcacctcatc
     6241 gcctgtaaga acaaaatctc cggtgatggc tgccttgcca tcaccggtcg gctggaaacc
     6301 gatagcattc gccacgccca tcggtccgac aggacttatc tgcattccgt tctcggtcac
     6361 cggatcgcgg cgcggcacag caaattgata gacaccgccg ttcgcctgcc ccttaacgcc
     6421 aatgatctga tcaagctgcg ccgtgtcgag atcgaccgcc ggcgcagggc cggccgtagc
     6481 cggagtcgcc agcggggtct tgctcgcgcc aagcgcatca cgaataacag ccgccatctt
     6541 cgccgggtcg ccatggccgc cgacatgcaa gtagaacgtc gcagggctgg cgcgcagcag
     6601 gtgattgtgc acggcggtga tctccaggcc accctcgatc agcttgagca tcaccggatt
     6661 gacttcggtc tcaagcagca cgaggtcgcc catgaccatg gcttccccat gcatcggctt
     6721 gaacgcaatc cagccgccaa gtgccagcgc cggcctgatc gtgacgccgt cgagtgccac
     6781 cgtgagatcg gtgcgtggaa agccataacg atgcacgtca cccgacacgg cgggcttcct
     6841 gccaaaggcg tcgtcgacct tctgccagtc gacttcctgt gcatgtgcgg aagtgacaaa
     6901 cggttccaaa aaaatctgcc agtcaatttt ctgtgcgttc gcgccaccgg caaacgaagc
     6961 tgcgaaaatt gaaactgtaa tccaaagcat cttacgcatt gttcggtcct cctttgttgg
     7021 gttgatcgcc ccgttcttgt tcttgtccgc gtcaagttgt cagtttggtc tgtgcttctc
     7081 gaccgttgcg tcgaaagttt cttgatcctt attcgcgcgc atccttatgt tggcgccgga
     7141 catccagact tcgttgcaat cgggtgactc cagatttggg ttcgacaagc agagtttgtt
     7201 gttctggatt ttccatgtcc cggtccattt ccgacccatt tcgctgctga gaagcacgcc
     7261 atcgggtcgg agatagctca cccaatgaga gctgtcggta atgtccttgc cgacgaccct
     7321 ggtgcggttt tccttttcgc caagcaattt gaacatttcc cgcgcgtgtg cgatcggtcc
     7381 acaaatcgtc gtaggcatca ggattgtcag cgcaacgatt tgtggcgttc ggcggttcat
     7441 gaccgaactc cgtcataggc gcgttgcacg gccggccaat tgatggccgc catgaaggtg
     7501 ccaacataat cggccgcttt ggcgccgaag tcgatgtgat aggagtgttc gtacatatcg
     7561 agcgcgagga tgggcgtgcc gccagccaag gtgtggcaat ggtccgccgc ccattgattg
     7621 acgagtttgc ggtcgcgcgg cgaccatgac agcagcaccc aaccggaacc tccgccgagg
     7681 gctttgccca tggcggtgaa ctccgcacgc caccgctcga acgagccgaa atcgcgagcc
     7741 agcgcttcct ggagggttgc atcgggctcg ctttgatcac cgagactgtc gaagaaatgc
     7801 tcgtgaagaa tcatcgagtt catggcgaca agttcttcgc gcttgagccc gttgatcagg
     7861 aagccgggag cgttggcgta atcgagctca gcgagcttct ctccgatgag attgagccgc
     7921 ttcacggcgc cgccatagtt gttttcgtag tggctgataa tcagccgctc ggacattccc
     7981 ttgattcgca cgggatcgca cccg
//
LOCUS       DASWGM010001045         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_547436,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001045 DASWGM010000000
VERSION     DASWGM010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547436"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VGA15_28125"
     CDS             <1..657
                     /locus_tag="VGA15_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX9591599.1"
                     /translation="SGKSTLLRCINWLERPDAGQIYLNGERVGINGAGIIMSDRDLSQ
                     IRTRIGMVFQHFALWPHLTVLQNLMEAPVQVQKRPKAEVRDEALALLAKVGLSEKRDA
                     FPARLSGGQKQRVGIARALAMRPDLLLFDEPTSALDPELVGEVLVVMRDLAREGMTMV
                     IVTHEMGFAREAATRVLFLDRGRAVETGSPERFFSDPETERARQFLQRYAGDIANGRR
                     "
     gene            687..1079
                     /locus_tag="VGA15_28130"
     CDS             687..1079
                     /locus_tag="VGA15_28130"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX9591600.1"
                     /translation="MHPIFHLLPDAPKPVAPYSHAVEAGAFVFVTGQLATDPDDDSLP
                     IPPGIEAQTHKVMDNLSRVLKGCGLTLANVVCVRIFLTDFNRDYARMNEIYATYFAAD
                     KRPARTTVGVTGLARGGIVEIDMIAVRS"
     gene            1090..1722
                     /locus_tag="VGA15_28135"
     CDS             1090..1722
                     /locus_tag="VGA15_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HEX9591601.1"
                     /translation="MTDSPIEIPARRGKAVFVSAGQIVTVINTHGEQVVDTWAFNRAD
                     LGEFMSNEHTRAHSLHLVPRPGDVLRTNRRRPILTLTEDTSGGIHDTLIAACDRYRYA
                     FLGVQDYHDNCTDNLFAGMAELGLVPAEVPSPLNLFMNIPWAADGTLGFAAPPRPIPG
                     GYVRLRAEMDLVIAFSACPQDILPINGTAGKPVEAHFTITEERFRAGARA"
     gene            1725..2537
                     /locus_tag="VGA15_28140"
     CDS             1725..2537
                     /locus_tag="VGA15_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HEX9591602.1"
                     /translation="MAQTKQEIAEANRRRYEKLRAAGQELTPKGLPEATALDGAPIAP
                     DTVTSREQVPAGWYATLRLRRGEALRIVDDSGRSSISLIGWREEDTSERINCADTVKV
                     QWSAAISKGRVILSDMGRVLLSLVEDTSGAHDLMVGGSTPASTLDAFGTPSRNTQENF
                     LAAAAKIGLSLRDIPPCVTFFAPVSVDAEGRFLWNSGRKRAGDFVDLRAEMNLALVLS
                     NCAHPLDPARPAASSPITLIRHRAPPPAHDDPCRTTSPEIVRAFVFTDRLYA"
     gene            2549..>3075
                     /locus_tag="VGA15_28145"
     CDS             2549..>3075
                     /locus_tag="VGA15_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018496126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEX9591603.1"
                     /translation="MTNPAGPLSIPDGSDAVYSHDIPAERPWSRVLEQGQTLRIVDSE
                     GQQAVDALLYCADDPAERYSAQDTLRVQGSAYIELGTRLVSNRGRVMAHISADSCGRH
                     DTSAGCCSCESNAVRFGEATKYQHACRENFLLELSKHGLTKRDIVANLNFFMNVPIDP
                     SGAFTVVDGISAPGN"
BASE COUNT          583 a          967 c          961 g          564 t
ORIGIN      
        1 tcgggcaaat cgacattgtt gcgctgtatc aactggcttg aacgccccga cgccgggcaa
       61 atctacttga acggcgagcg ggtcggcatc aatggtgccg gcatcatcat gagcgaccgc
      121 gatttgtcgc agatcagaac ccgcatcggc atggtgtttc agcattttgc gctgtggccc
      181 cacctgacag tgctacaaaa tctgatggaa gcgccggttc aggtccagaa gcggccgaag
      241 gccgaggttc gcgacgaggc gctggccttg ctcgcgaagg tcgggctcag cgagaagcgc
      301 gacgcctttc cggcgaggct gtcgggcggg cagaaacagc gggtcggcat cgcgcgggcg
      361 ctggcgatgc gcccggatct cctgcttttc gacgaaccca ccagcgcgct cgatccggaa
      421 cttgtcggcg aagtgctggt cgtgatgcgc gatctcgccc gcgagggcat gacgatggtg
      481 atcgtcaccc acgaaatggg attcgcgcgc gaagcggcga cgcgagtact gtttctcgat
      541 cgcggccggg cggtcgaaac cggatcgccg gagcgttttt tctccgatcc cgaaaccgaa
      601 cgcgcgcgcc agtttctcca gcgctatgct ggtgacatcg cgaacggcag gcgctgagaa
      661 caacttgagc caagaggacg tccaccatgc atccgatctt tcatctgttg cccgacgcgc
      721 cgaagccggt cgcgccctac agccatgccg tcgaggccgg agcgttcgtc ttcgtgaccg
      781 gccaactcgc gaccgatccg gacgacgatt ctctgccgat tccgcccggc atcgaggcgc
      841 agacgcacaa ggtgatggac aatctaagcc gcgtgctcaa aggctgcggc ttgacgctcg
      901 ccaacgtggt ttgcgtgcgc atcttcctga ccgacttcaa ccgcgattac gccaggatga
      961 acgaaatcta cgcgacttat ttcgctgccg acaagcgtcc ggcccgaacc acggtggggg
     1021 tgacgggcct cgcccgcggc gggattgtcg agatcgacat gatcgctgtc agatcgtgag
     1081 gcgcctggca tgacggactc gccaatcgaa attccagcaa ggcgtggcaa ggccgtcttt
     1141 gtatcggccg ggcagatcgt caccgtcatc aatacccacg gcgaacaggt ggtcgatacc
     1201 tgggcgttca accgcgccga tctcggcgag ttcatgtcga acgagcatac ccgtgcgcac
     1261 agcctgcatc tggttcccag gccgggcgat gtcctgcgga ccaacaggcg ccggccgatc
     1321 ctgacattga ccgaagacac ctccggcgga attcacgaca cgctgatcgc ggcctgcgac
     1381 cgttaccgct acgcctttct cggcgtgcag gactatcacg acaattgcac ggacaatctc
     1441 tttgccggga tggccgaact tggcctcgtg ccggcggaag tgccgagccc gctcaacctg
     1501 ttcatgaaca taccatgggc ggcggatggt acgctcggtt tcgcggctcc gccgcgaccg
     1561 atacctggcg gctatgttcg gctgcgggcc gagatggatc tggtgatcgc gttttccgcg
     1621 tgcccgcagg acatcctgcc gatcaacggt acggccggaa aacccgtcga ggcgcatttt
     1681 acaatcaccg aagagcgttt cagggccggg gcgagagcat gacgatggcg caaacgaagc
     1741 aggagattgc cgaggccaat cggcggcgct acgaaaaact tcgggcggcg gggcaggaac
     1801 taaccccaaa aggcttgccc gaggcgaccg ctctggatgg cgcaccgatc gcgccggaca
     1861 ccgtcaccag ccgcgaacag gtccccgccg gctggtacgc cacgcttcgg ttgcgacgcg
     1921 gcgaggcgct gcggatcgtc gacgattccg gccgctccag catctcgctg atcggctggc
     1981 gcgaggaaga tacttccgag cgcatcaatt gcgccgacac cgtcaaggta cagtggagcg
     2041 ccgcgatctc gaaaggccgc gtcatcctgt cggacatggg acgtgtattg ctgtcgctgg
     2101 tcgaagatac cagcggcgcc catgatctga tggttggtgg ctcgacaccg gcctcaacgc
     2161 tggatgcgtt cgggacacct tcgcgcaaca cccaggagaa tttccttgcc gccgcggcca
     2221 agatcgggct cagcctccgg gacattccgc cctgtgtgac gtttttcgct cccgtctcgg
     2281 tcgacgcgga gggccgcttc ctgtggaatt caggccggaa gcgggcaggc gacttcgtcg
     2341 atctgcgcgc cgagatgaat ctcgcgctgg tgctgtcgaa ttgcgcccat ccgcttgatc
     2401 cggcgcggcc cgctgcgtcc tcgccgatca cgttgatcag gcaccgcgcg ccgccacccg
     2461 cgcatgacga tccctgcagg acaacgtcac cggaaatcgt ccgcgccttc gtattcacgg
     2521 atcggctcta cgcctgagga gagtttcagt gaccaatccc gcaggcccgt tatcgattcc
     2581 agacggctcc gatgccgtat actcccacga cattccggcg gaacggccgt ggtcgcgcgt
     2641 gttggagcag gggcagacct tgcggatcgt cgacagcgaa gggcagcaag cggtcgatgc
     2701 gctgctttat tgcgccgatg atccggcgga acgttacagt gcgcaggata cgctgcgtgt
     2761 tcagggatcg gcttatatcg aactcggaac ccggctggtc tcgaacaggg gccgggtgat
     2821 ggcacacatt agcgcggact catgcggccg acacgacacc tcggcaggtt gttgctcctg
     2881 cgaaagcaat gcagtccgct tcggggaagc gacaaaatac cagcacgcct gccgcgaaaa
     2941 ttttcttctc gagctgtcga agcacggact gaccaagcgc gacatcgtcg caaacctgaa
     3001 tttcttcatg aatgtgccga tcgacccttc cggagctttt acggtcgtcg atggcatctc
     3061 ggcccccgga aatta
//
LOCUS       DASWGM010001046         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1244336,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001046 DASWGM010000000
VERSION     DASWGM010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244336"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1028)
                     /locus_tag="VGA15_28150"
     CDS             complement(27..1028)
                     /locus_tag="VGA15_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX9591604.1"
                     /translation="MDRRQTLKLIGGAAAMAGAGLPSLAFAEAQKEMVTVVKIAGIPW
                     FNAVEKGILKGAKDFDINATMVGPANVDPAQQVKLLEDLIAKKVNVIGLVPLDVKVCE
                     PVLKRAQAAGIKVITHEGPEQEGRDWNVELIDSVRFGEVQMERLAKDLGGEGEYVVYV
                     GTLTTPLHNKWADAAIAYQQRKFPNMKLVADRFPGADEIDTSQRTTLDVIKAYPNLRG
                     ILGFGSNGPIGAGNAVRQQRLAKKIAIVGTVLPSQAKSLIADDIIREGFLWNPTDAGY
                     AMVAVAKLVLDGKSITDGVEVPGLGKAAVDVAGKQIKVDKIMRINKETIDGLISAGL"
     gene            1257..>1513
                     /locus_tag="VGA15_28155"
     CDS             1257..>1513
                     /locus_tag="VGA15_28155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591605.1"
                     /translation="MTMSFDMAREMSGKSIACAIADDDAVVRPFDRTARRRPACSSQS
                     GARLPKRDATLLLKGVGDRVMRYALGGIAHEPHGSLKPASQP"
BASE COUNT          264 a          474 c          457 g          318 t
ORIGIN      
        1 aggaggcggc ttgggctggt gtgttcctag agccccgcgg agatcagccc gtcgatggtt
       61 tccttgttga tccgcatgat cttgtcgacc ttgatctgct tgccggcgac atcgaccgcc
      121 gctttgccaa ggccggggac ctcgacgcca tccgtgatcg acttgccgtc gagcacgagt
      181 ttggcgaccg cgaccatcgc gtaacctgca tcggttggat tccacaggaa gccctcgcgg
      241 atgatgtcgt ccgcgatcag cgatttcgcc tggctcggca gcacggtgcc cactatggcg
      301 atcttcttgg cgaggcgctg ctgacgcacc gcgttaccgg cgccgatcgg cccgttcgag
      361 ccgaagccga gaatgccgcg caagttagga taggccttga tcacgtccag cgtcgtgcgc
      421 tggctggtgt cgatctcgtc ggcgccgggg aaacggtcgg cgacgagctt catgttcggg
      481 aatttcctct gctgataggc aatcgcggcg tcggcccact tgttgtgaag cggcgtcgtc
      541 aacgttccga catagaccac atactcgcct tcgccaccga ggtccttggc gaggcgctcc
      601 atctgcactt caccgaagcg aacggagtcg atcagttcca cgttccagtc acgaccttcc
      661 tgctccggac cttcatgggt gatgaccttg atgccggccg cctgggcgcg cttgagcacc
      721 ggctcgcaaa ccttgacgtc gagcggcacc aggccgatca cgttgacctt cttggcaatc
      781 aggtcttcaa gcaacttgac ctgctgcgcc ggatcgacgt tcgctgggcc aaccatggtg
      841 gcattgatgt cgaaatcctt cgcgcctttc aggatccctt tttcaacggc gttgaaccag
      901 ggaatgcccg cgatcttcac gaccgtgacc atctccttct gagcctccgc gaaggcgagg
      961 ctaggcaaac cggcaccggc catcgctgcc gcaccaccaa tcaatttgag tgtctggcgt
     1021 ctgtccattg ttccctccca ggaaactatt tcgctgccgc tcccttgatc cggcacatgc
     1081 cgggcgggag cagtcggctt cattcttgat tttcggcaga aacttccgcg cttccggccg
     1141 cagaaccttg ttgagggatt agagaccaac gctccagaag gtgtcaagac cgcgcacgtt
     1201 ccggcagtgc cgcccgcacc ggccgtggtg tttgttgatc gtcaggaatt gcgaaggtga
     1261 cgatgagctt cgacatggca cgcgagatgt cgggaaaatc gattgcctgc gcgattgccg
     1321 atgatgacgc agtggtgaga cctttcgacc ggacagcgcg gcgtcgcccg gcatgctcat
     1381 cgcagagcgg agcgcggctg ccgaagaggg acgcgacctt gttgctcaag ggcgtcggcg
     1441 atcgcgtcat gcgctacgcg ctcggtggca tcgctcacga gccgcatggt tccttaaagc
     1501 cggcaagcca gcc
//
LOCUS       DASWGM010001047         8501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1244739,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001047 DASWGM010000000
VERSION     DASWGM010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244739"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /gene="trpA"
                     /locus_tag="VGA15_28160"
     CDS             <1..134
                     /gene="trpA"
                     /locus_tag="VGA15_28160"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508670.1"
                     /note="catalyzes the formation of indole and
                     glyceraldehyde 3-phosphate from indoleglycerol phosphate
                     in tryptophan biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX9591606.1"
                     /translation="ALVDALRGSLDAQGRATAKTVGTVADLVAALAQGVRGAKQAAE"
     gene            237..1181
                     /gene="accD"
                     /locus_tag="VGA15_28165"
     CDS             237..1181
                     /gene="accD"
                     /locus_tag="VGA15_28165"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX9591607.1"
                     /translation="MNWLTNVVRPKIRNILRRETPENLWIKCPDSGQLVFYKDVESNQ
                     FVIPGSNYHMRMGAVARLKSIFDNETWFDVALPEVTADPLKFRDERRYADRIRDARAR
                     TGLNDAIKVGYGKLEGAGVVIAVQDFDFMGGSLGMAAGEAIVRGLELAVEKKAPFIVF
                     AASGGARMQEGILSLMQMPRTIVGVQMLREAKQPYIVVLTNPTTGGVTASYAMLGDVQ
                     LAEPGALIGFAGARVIEQTIREKLPEGFQRAEYLKDHGMIDMVVHRHDLRPTLARLCR
                     LLSRSPAIETASKPAPPIAIPAEIVPVVADVAPAAPHA"
     gene            1178..2521
                     /locus_tag="VGA15_28170"
     CDS             1178..2521
                     /locus_tag="VGA15_28170"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX9591608.1"
                     /translation="MNAPAAQPSPPLGELIARLSALHAKRIDLSLERMQGLLERLDHP
                     ERRLPPVIHVAGTNGKGSTIAYLRAILEAANLRVHVYTSPSLVRINECFRLGRTGGGA
                     LVGDDELRSTLEHCERANAGAPITIFEILTAVAFCLFAQYPADVVLLEVGLGGRLDAT
                     NVIDAPLATVMAPVSMDHTEFLGHTLTAIAGEKAAIIKRRVPVICAEQFPETMAVIEV
                     QAERMRAPLHAVGQQWHVGVECGRLVYQDDRGLMDLAAPKLFGRHQFDNAGLAIATLR
                     AQSLFEIGTTAFEAGIVNAEWPARMQRLASGALLEQAPPGCEIWLDGGHNAEGGRVVA
                     AAMGDLEERVSRPLVVIVGMMANKDGGAFLANFAGLTRHIIAVQIPDRDNAMPPDRLA
                     DAARSLGMRVEISASVEAALRSLARLAYEVPPRILITGSLYLAGHVLAANGTPPG"
     gene            2530..3270
                     /locus_tag="VGA15_28175"
     CDS             2530..3270
                     /locus_tag="VGA15_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX9591609.1"
                     /translation="MRFAAIADVHGNYLALAAVIADIRAQNIDEIVNLGDMASGPLDA
                     RRTMDALMALDAVHVLGNHDRYLIDRPPEKMGSWDRPAHGQLEAHHLDWLRAVPPTLV
                     FRDEVFLCHATPDRDEVYWLETVLPDGTVRMSSLEEIEKAAERVTQRLILCGHTHIAR
                     AVRLGDGRMVVNPGSVGSPGYRDVHPYPHVIEAGTPDARYAILEFSDGVWRATFRHVP
                     YDHDAMAALARRNGQPELASALATGWIR"
     gene            3526..3954
                     /locus_tag="VGA15_28180"
     CDS             3526..3954
                     /locus_tag="VGA15_28180"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX9591610.1"
                     /translation="MNGEQVWRGEYGIETSATPDIIWSIFRDAPGWKTWNAGIEHIDI
                     DGPFSTGTCFTMKPPGEDPLRSRLVEVSKNECFVDETRIGYLAITVAHRIESLGARRT
                     RIAYVVEARGPEASEIGPAVASDFPEILAALAKLAEARSA"
     gene            3951..4391
                     /locus_tag="VGA15_28185"
     CDS             3951..4391
                     /locus_tag="VGA15_28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX9591611.1"
                     /translation="MTAWLGVVSRAHVRTGVAGRFAQLCHGKAQPLRRMRRGDWLVYY
                     SPTIEMGGAALRAFTAIGQVEGPEVYRYDMGGGFVPYRRDVRYADAQEVAIDMIKPRL
                     ELCARPNWGTALRRGQVPLEAADFATIAAAMGVRQTKRFGVGLE"
     gene            4499..4876
                     /locus_tag="VGA15_28190"
     CDS             4499..4876
                     /locus_tag="VGA15_28190"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX9591612.1"
                     /translation="MALQPYGLTHSQFVVLATATWFGASETLTQSRISQLSGIDPMTT
                     SQVLRALEAASLIQRVDHPKDPRAKSIMVTRVGRDLARKAIVVVEETDAAFFEPLASD
                     TARLVEMFQALVRDNERMEADEN"
     gene            complement(4980..5300)
                     /gene="trxA"
                     /locus_tag="VGA15_28195"
     CDS             complement(4980..5300)
                     /gene="trxA"
                     /locus_tag="VGA15_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX9591613.1"
                     /translation="MAVGKVSDADFEAEVLKATGPVVVDFWAEWCGPCRMIAPALDEI
                     SGAMGDKVKIVKLNVDESPKTASKYGVMSIPTLMIFKGGEMASRQVGAAPKQKLQQWI
                     TAAV"
     gene            complement(5372..8501)
                     /gene="addA"
                     /locus_tag="VGA15_28200"
                     /pseudo
     CDS             complement(5372..8501)
                     /gene="addA"
                     /locus_tag="VGA15_28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603936.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
BASE COUNT         1507 a         2698 c         2757 g         1539 t
ORIGIN      
        1 ccgcgctggt cgatgccttg cgtggcagcc tcgatgccca gggtcgtgcg acggccaaaa
       61 ccgtcggaac ggtggccgat cttgtggccg cgctggcgca gggcgtgcgg ggcgcgaaac
      121 aggccgcgga ataagccaca attgcgggag atagaccgca agctgcggct tgcccggcac
      181 cgtccaggcc gccatatatt ccatgatggc tgcagaatcg catctcggag cgaaacatga
      241 attggctcac caacgtcgtc cggccgaaaa tccgcaacat cctgcgccgc gagacgccgg
      301 agaatctgtg gatcaaatgc cccgattcag ggcagctcgt gttctacaag gacgtcgaaa
      361 gcaaccagtt cgtcatcccc ggctcgaact accacatgcg gatgggcgcg gtggcgcggc
      421 tgaaatcgat cttcgacaac gagacctggt tcgacgtggc gctgcccgag gtgaccgccg
      481 atcccctgaa gtttcgggat gaacgcagat atgccgaccg catcagggat gcccgcgccc
      541 ggaccgggct gaacgacgcc atcaaggtgg gctacggcaa gctcgagggc gccggtgtcg
      601 tgatcgcggt gcaggatttt gatttcatgg gcggatcgct cggcatggct gcgggggaag
      661 cgatcgtgcg cgggctcgaa ctcgcggtcg aaaagaaagc tccgttcatc gtgttcgcgg
      721 cttccggcgg cgcccgcatg caggaaggca ttctgtcgct gatgcagatg ccgcgcacca
      781 ttgtcggcgt gcagatgctg cgcgaggcga aacagcccta catcgtggtg ctgaccaatc
      841 caaccaccgg cggtgtcacc gcctcctatg ccatgctcgg cgacgtgcag cttgccgagc
      901 ctggcgcgct gatcggcttt gccggcgcgc gggtaattga acagaccatc cgcgagaaat
      961 tgccggaagg attccagcgc gcggaatacc tcaaggatca cggcatgatc gacatggtgg
     1021 tgcaccgcca tgatctgcgc ccgacgctgg cgcggctctg tcgcttgctg agcaggtcgc
     1081 ccgccataga gaccgcgtcg aagcccgcgc caccgatcgc tattccggcc gagatcgtcc
     1141 cggtcgttgc ggatgtggcg ccggccgcac cccacgcgtg aacgcgcctg cggcccaacc
     1201 atcgccaccg ctcggcgaat tgatcgcgcg gctgtccgcg ctgcatgcca agcgtatcga
     1261 tctcagtctg gagcggatgc aggggctgct ggagcggctc gaccatcccg aacgcaggct
     1321 gccgccggtg attcatgtcg ccggcaccaa tggcaagggg tcgacgatcg cctatttgcg
     1381 ggcgattctg gaagccgcaa acctgcgcgt tcacgtctat acgtcgccct cgctggtgcg
     1441 gatcaacgaa tgctttcgcc tcggcaggac cggcggcggc gcgctcgtcg gcgatgacga
     1501 actgcgcagc acgctcgaac attgcgagcg cgccaatgcg ggggcgccga tcactatttt
     1561 cgaaatccta accgcggtgg cgttttgtct gttcgcgcaa tatccggccg acgtcgtgct
     1621 gctggaagtc ggtctcggtg ggcggcttga cgctaccaac gtgatcgacg cgccgctggc
     1681 gaccgtgatg gcgccggtca gcatggacca caccgaattt ctggggcaca cgctgacggc
     1741 gattgccggc gagaaggccg ccatcatcaa gcgccgggtg ccggtgattt gcgcggagca
     1801 atttcccgag acgatggccg tgatcgaggt gcaggccgaa cgcatgcgcg caccgttgca
     1861 tgcagttggc caacagtggc acgtgggtgt cgagtgcggc cgactggttt atcaggacga
     1921 tcgtggcctg atggatctcg cggcaccaaa actgttcggc cggcatcagt tcgacaatgc
     1981 cggcctcgct atcgcgacgt tgcgcgcgca aagcctgttc gagatcggta ccacagcctt
     2041 cgaggccgga atcgtcaacg ccgaatggcc ggcgcggatg cagcgtctgg cgtcgggcgc
     2101 gctgctggag caggcgccgc cgggttgcga gatatggctc gatggcggac ataatgccga
     2161 gggcgggcgc gtcgtcgcgg cggccatggg cgatctcgag gagcgggtat cgcgtccgct
     2221 ggtcgtgatc gtcgggatga tggccaacaa ggatggaggc gcgtttctcg ctaatttcgc
     2281 tggcctgacg cgccacatca tcgcggtgca gatcccggat cgcgacaacg cgatgccgcc
     2341 ggatcgactg gcggacgccg cgcgctcgct cggcatgcgg gtggaaatct cagccagtgt
     2401 cgaggcagcg ctgcgctcgc tcgcgcgcct tgcctatgag gtgccgccgc gcatcctgat
     2461 cacgggatca ctttatctcg ccggccatgt gctcgccgcc aacggcacgc caccaggata
     2521 agcctgacaa tgcgctttgc cgcgattgcc gatgtgcacg gaaattatct cgcgcttgca
     2581 gccgtgatcg ccgatatccg cgcccaaaac atcgacgaga tcgtcaacct cggcgacatg
     2641 gcgagcggcc cgctcgacgc gcggcggacg atggatgccc tgatggcgct cgacgctgtg
     2701 catgtactcg gcaatcacga ccgctatttg atcgaccggc cacccgagaa gatgggatcg
     2761 tgggaccgcc ccgcgcacgg gcaactcgag gctcatcatc tcgactggct gcgcgcggtg
     2821 ccgccgacgc ttgtgttccg cgatgaggtg tttctctgcc acgcgacacc ggaccgcgac
     2881 gaggtctatt ggctggagac cgttttgcct gatggcacgg tacggatgtc gtcgcttgaa
     2941 gaaatcgaaa aggcggccga gagagttacg cagcggctga ttctctgcgg ccacacccac
     3001 atcgcgcgcg cggtccggct tggcgacgga cgcatggtcg tcaatcccgg cagcgtcggc
     3061 tctcccggct atcgcgatgt ccatccgtat ccgcatgtca tcgaggccgg cacgccggac
     3121 gcgcgttacg cgatcctgga attttccgat ggcgtgtggc gcgcgacgtt ccggcatgtg
     3181 ccgtacgatc atgatgcgat ggccgcattg gcgcgtcgca acggtcagcc tgaactcgca
     3241 tccgcgctgg cgacgggatg gatcaggtag cgacgaaacg cacgtgtccc ggacgcggtg
     3301 cagcgtctct tcgacgatgc accgcagagc cgggacccat cgtttatctg atacggcatg
     3361 ggccccggat cagcagcgcg tcacttcgtg ccgcgcagca tccgggcacg gcagaaatcc
     3421 agccaacacg caccacaact caaacggccg atgatcggca ggtgcccctt gacaattatg
     3481 tatgtgcgca tattatatgc acacatatat aatggagagg cgacaatgaa cggggagcag
     3541 gtttggcgtg gcgaatacgg gatcgagacg tcggcgaccc cggacatcat ctggagcatt
     3601 ttccgcgatg cccccggctg gaaaacctgg aatgcgggca tcgagcacat tgatatcgat
     3661 gggccgttct cgaccggaac gtgcttcacg atgaaacctc ccggggagga tccactgcgg
     3721 tcaaggcttg tcgaggtttc caagaacgag tgcttcgtgg acgaaacgcg cattggatac
     3781 ctcgcgatca ccgtcgcgca tcgaatcgag tccctcggag ccaggcgcac acggatcgcc
     3841 tacgtggtcg aggctcgcgg acccgaagcc tccgagatcg gtcccgctgt tgcgtcggac
     3901 tttcctgaga ttctcgcagc gctcgccaaa ctcgccgaag cgaggtcagc atgacggcct
     3961 ggcttggcgt ggtttctcgc gcccatgtcc gaacaggcgt agctggccgc ttcgcgcaat
     4021 tgtgccacgg caaggcgcag ccacttcgcc ggatgcgccg gggcgactgg ctcgtctact
     4081 attcgccgac gatcgagatg ggcggcgccg cattgcgcgc gttcacggcc atcggacagg
     4141 tcgaaggtcc ggaggtctac cggtatgaca tgggcggcgg atttgtcccg tatcggcggg
     4201 atgtccgcta tgcggatgcc caggaggtgg cgatcgacat gatcaagcca aggctggagc
     4261 tctgcgcgcg tcctaactgg gggacggcgc ttcgccgcgg tcaggtgccg ctcgaggcgg
     4321 ccgacttcgc cacgatcgcc gccgcaatgg gcgtgcggca gacgaaacga tttggggtgg
     4381 ggttggaatg aagcgcaggt tccggggccc accgttaccg acgcgggttg ccggtccggc
     4441 ggaaagccca ggctttctgc tgtggaagat atcgaacgct tggcagcggc gacagcggat
     4501 ggctcttcag ccttatgggc tcacccattc ccagttcgtg gttctggcga ccgcgacctg
     4561 gttcggcgcg tccgaaaccc tgacgcagtc gcggatttcc cagttatccg gcatcgatcc
     4621 aatgaccacg tcccaggttc tacgcgcgct ggaagctgcc tccctgatcc aaagggtcga
     4681 tcaccccaag gatccgcgcg ccaagtcgat catggtcacg cgggtcggtc gtgatcttgc
     4741 ccgcaaggca atcgtcgtgg tcgaggagac cgacgccgcc tttttcgaac cgctggcgtc
     4801 tgataccgcg cgacttgtcg agatgttcca ggcgctcgtt cgagacaatg aacgaatgga
     4861 agccgatgaa aattaacggg tctcgtttag ccaaggctcc gcacggtcgg tgccgcctac
     4921 caaaaaaacg gccggctatt cgccggccgt cttcgggaat aattgctgtg cgcgagcgat
     4981 cacaccgcgg cggtgatcca ctgctgcagc ttctgcttcg gcgcggcgcc gacctgacgc
     5041 gaggccatct cgccgccctt gaagatcatc agcgtcggga tcgacatcac gccgtatttc
     5101 gaggccgtct tcgggctctc gtcgacgttc agcttcacga tcttgacctt gtcgcccatc
     5161 gcgccggaaa tctcgtcgag cgcgggtgcg atcatgcggc aggggccgca ccattcggcc
     5221 cagaaatcca ccacgaccgg gccggtcgcc ttgagcactt cggcttcgaa atcggcgtca
     5281 gaaaccttgc caacggccat tgggtacctc gttcggtttt aggagtggat gcggcctcaa
     5341 gaatcgccgc ctggatcatg catccaacgt atgaacgcgg ccctgccggg tcaagctcgg
     5401 tcatcccatt ggatatggcc ggccagctgc gcgtccaacg caggagcaga aatctccatc
     5461 aattcaggcg tttcggtcca gagcagcgct gcgcggaccg gcaatcgggg ataaagcttc
     5521 tgcagcacca cgcggtagag cgcgagctgg cggacatagc cgggaggcgc ctcggccgga
     5581 agcttcggcg gggcgtgatt ggtcttgaaa tcgacgatca ggacctcgtc cggcgtaacc
     5641 accagccggt cgatctgtcc cgaaaccagg gcggccgagc gtccgggccg ctccagcctt
     5701 ccgacgatcg agacctcggc gcggctatgg ggcgcgaata ccggcgcgaa ccgcgtgtcc
     5761 caaatcaagg ccagcacccc ttccgcgagc gcctcgcggt cgcccgcggt ccagccgtcg
     5821 gcgttgcggg tgaggtattt tagcgccgcg tcgcggcggc gctcgagggc gacatcgggc
     5881 agcgactgca gcagccggtg caccagcgtg ccgcgctgca aggcgcgccc gcgcgcctgg
     5941 atggattccc cggttcgggt acgatgacct tcgttctcgg caggatccga gggatgcagc
     6001 agacgatcgg cgcggaggtc caacggcgcg gcggcgcgta accacagcgg cagttcggtc
     6061 ggagcggcgg tcgctggagc tgcgactttg ccgacgggcg ccgcgacatc ctccagccgt
     6121 gaatagcgtc ttacggggcc gtccggcgtc tcgatcgtcc gctcatgcaa tccggaattg
     6181 ccgaggccct tggcgatcag gtcgtaccag gagaatttgc gtatcgtatt catgttgccc
     6241 ggcatgcagc cgccgacgat caggcggtct gccgcgcgcg tcatcgccac ataaagcagc
     6301 cgacggtact cgtcttcggt gtcgctcagc atcgccgcgc gggccgccgc gacctccgca
     6361 ggatcgtcag cttttttgcc ggcccagacc acgacgccgg gtgcgtgagg cgccgcattg
     6421 ccctgcggca gatggatcag tttgaggcgc tgggtatccg caggcgacgt cgtggtgtcg
     6481 accagaaaga ccaccgacgc ttccagcccc ttggcgccgt gcaccgtcat gacccggact
     6541 tcatcgcggg aaatttccat gtcgcgcttc acctcgaggt cggcggcgcg cagccacgcc
     6601 atgaaaccct gcagcgaagc gggcgccttg cgctcatagc cgagcgcgag ttcgagaaat
     6661 tcgtcgagcg cgtcgttggc ctcgtgcccg agccgtttga ggattcgcgc gcggccgccg
     6721 tcgccgccga gcagccaggc atagaacgca aacggcgttt cctgcagcac gcgcgcctcg
     6781 caggcgctga ggcgggcaag cgcggcctcg aatttgctgt cggtcgccgc gtgaaggttc
     6841 aatgcatcgc gcagcgatcc cttgcgctgc caggcgagct tgaacagatc gtcgtcggta
     6901 aggccgaaca acgggctttt cagtgccacc gcgagcgcca gatcgtcgtg cgacaacaat
     6961 agcgcgtcgg ccagattcat cagatcgatg atcgcgatat gctcggtcaa tttcaacctg
     7021 tcggcgccgg cgaccggaat attggcgtgc ttcagcgcct ggatcaccgc gtcaaaggcg
     7081 ttgccgcgcc gccgcaccag caccagcatg tcgccgtagc gcaggggacg gcgatcggcg
     7141 gttgggccgg tcatggcgcc gctttcgacc agcgacttga tctcggcctg aatgcgcctg
     7201 gccagcctga cttccgggct ggtctccgaa accgcgtcga acggcgcccg ccagccttcg
     7261 atatcctgtc gtccgtccgg cagctgcaac tcccagaggt caatcaggct cggcccggca
     7321 tcggcgaggt agtcgtggat cgggtagccg gtttcggcgg aatggatgct gcggtagatc
     7381 atttcttcgc gaaatacatg atcgaccgat tgcaggatcg caggccccga acggaacgag
     7441 taagtgaacg agaccggatc gaatttcagg ccggcatcga taaacttctt ctccagcccc
     7501 ctgcggcggc tatcgaattc gcgcggggcc gcaccctgaa acgaaaaaat cgactgcttc
     7561 tcgtcgccga ccgcgaagat cgtgcgggtg acgccgtcgc gcgcgccttc gccggatgtg
     7621 aattcggaaa tgatatgcgc gacgatatcc cattgccggg ggctggtgtc ctgggcctcg
     7681 tcgatcagca cgtgatcgac gccgcgatcg agcttgtaat gcacccaccc cgacgacacc
     7741 cgatccagca tctccagcgt cttgtcgatc agatcgtcat agtcgagcaa gccacgttcc
     7801 cgcttctcgc gccggtaatt cgcagccgcc gcggttgcga tcgccagcag cgcttgcgtg
     7861 cggtcgcggg tggtgaccgc gcggcgccgc tcgatcagag gtccgatgcg ctggccctcc
     7921 cgctcgaaca agtttgcgac aatcgcattg ttgtcgccga atttcttcgt caccaccgtc
     7981 ttgcggggtg tccgctcgtc ggtaaggaat atgccgagat attcttcgac ttgcgcagcc
     8041 ccgatcgatg cgagcgctgc gcgaagccga tccgcgtggc cctgctcgga tttggcgcca
     8101 ccgtcgagaa tcgcggcgac ttccttccac cgcgatctcg gcagatgtgg cccgtcgaca
     8161 atctcgcgct cgacgtcttc gatgaggtca atgggatcga cgccaagtgc ggcggagatc
     8221 tgcgccgccg cagcctcgac gctgccggcg ctatcggtcc aggccatgaa gtgatcgcgg
     8281 ctcaagcagg cttcgcgcac cacgtccttg aaggtgacat cggcagcact ggccatggca
     8341 acattgagcg cccggcctgt cgcgctatcg ggatcgcggg acgcgtcgag cagcaccttg
     8401 agattggcgc gctccatcat ctcgttctgg tcgcgctcgt cgagcaccgt gaagcgcgcc
     8461 ggcacgttcg cctcgaacgg aaattgctgc agcagccggg t
//
LOCUS       DASWGM010001048         4797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_822263,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001048 DASWGM010000000
VERSION     DASWGM010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822263"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VGA15_28205"
     CDS             <1..462
                     /locus_tag="VGA15_28205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591614.1"
                     /translation="AFWGHASVRAHALEAGAFVLSACGQITEADIPDDFPHKGRMNIN
                     YAKGGSSVIAPLGVPLVGPVEGPAIVRAELQAWMIKAWKAIIDTNGHYARPDLVGLSV
                     TNHIPSQHPVVLRTQPKPALLAEAAERNGVEPALVESVMHDAARLDAPVNR"
     gene            complement(563..2485)
                     /locus_tag="VGA15_28210"
     CDS             complement(563..2485)
                     /locus_tag="VGA15_28210"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX9591615.1"
                     /translation="MMTIAIKAYANADDVLIAWQPDTWSDAWVGFQLERRNDTTQAAT
                     VLVNRIPPKAGEGPVQPTGISSAQSPIRRCIWTDHSVVATDNVSYRVTAMTSAANGAF
                     APDATSVTDWTAPLIASGDAGGGLQAYFNRGTLMSQIVSRFVNGDVSDASLRKFLDDL
                     ATSGFQARLYLSGDALHEILAFLRDADRRGSTIHAAIYEMNDQQLVDALKPFGGRGHL
                     LLGNGSATRPTVAPELSSAGLEVKHRDLSHHGKSSPSVHNKFVVESDAHGNASRVLTG
                     STNWTTTGLCTQLNNVLIVDDTVIASRYLDQWGKLVAAGDNLPAGLIASNSNPTTHNN
                     ISVYFAATNGEAEFKPALDLIAGAKQGALFLMFMPGQSPLLSALLDRAVQNNIYVRGV
                     VSTIMPTQNGDIVAVGGQVVKSGAPAQSFHDDVRLPAGVSAKDHPSWEETEFNVQEMH
                     AAHLMAIVHSKAIVIDPFSDQCAVITGSHNFSDSASQKNDENLVIVRGNKKLTQAYAL
                     HINGVYDHYSWRAFLGSGGDPDQIFKPLDGWMPGGSRAQELDFWMNQPIPPQRTKQGT
                     SSAKSKAGKTPKKSKTSPKKKAAPKKSSKTSPKKSKAGRKKKATPASRAKAARKSKKT
                     GRAAPKEAKRKPARGR"
     gene            2678..3538
                     /locus_tag="VGA15_28215"
     CDS             2678..3538
                     /locus_tag="VGA15_28215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX9591616.1"
                     /translation="MSEIEKITTSPRFTFDALVTGHRGSPLVLLLHGFAESMNCWRAQ
                     MAALAAANYRAVAPNQRGYSPGARPDTGDSANYHIDRLMDDAMAIVGASGYGDRRFHL
                     VGHDWGGSIAWALADRFPDRLASLTVLSRPHPNAFNRALQMQDGDQAQRSRHHKAFLE
                     PDAGELLLADNAKWLRERLSAAGVPSAAIEKHLSVLGNKAAMEAALAWYRARGAIRSP
                     LGPIKVSTLYIWGDADDTVGRVAAEGTGDFITAPYRFEVLPGVGHFAADQVPDRVSEL
                     LLQHVATYPA"
     gene            complement(3526..4503)
                     /locus_tag="VGA15_28220"
     CDS             complement(3526..4503)
                     /locus_tag="VGA15_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9591617.1"
                     /translation="MNGVTINQLRCFDAVVTEGGFQAAATKLRRTHSAVFTSIKNLES
                     QLGLRLLDRDGYRVTLTDHGRSFHDRARGLLADLALLGKHARQLAMGEEAELRIVLGD
                     LCPLPEVLGLLRHFFKGCPETRLHLYFEAISGPWERLFDNEADLIIHHIDKSDPRLEF
                     IDLFAGTLLPVVAPNFLPFPISKGITPEQMRGHVQCVIRDTALRWPARDYYLIEGART
                     WTVSDQQMKKEIILQGMGWGHMPGFMIADELRDGRLLSIAGKHLRGGGGEIVAARRRD
                     APHGPIANRLWSFIAAQAPALNATIKRRGPKSRGHFRVGEVPRPIHQAG"
     gene            4617..>4797
                     /locus_tag="VGA15_28225"
     CDS             4617..>4797
                     /locus_tag="VGA15_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX9591618.1"
                     /translation="MITLHGFGRFFGTPDSSPFVIKVMMLLKLAGVAYREVQGNPFTA
                     PRRLLPYLRDEDVTVA"
BASE COUNT          890 a         1478 c         1469 g          960 t
ORIGIN      
        1 gcattctggg ggcatgcatc cgtgcgggcg cacgcgctgg aggccggtgc gttcgtcctc
       61 tcggcgtgtg gacagattac cgaagccgat attcccgacg atttccccca taaaggccgc
      121 atgaacatca actacgccaa aggaggatca agcgtcattg cgcctctagg agtcccgctg
      181 gtggggccgg tggagggccc agccatcgtt cgcgcggaac ttcaagcctg gatgatcaag
      241 gcgtggaagg ccatcatcga taccaacggc cactatgcgc gaccggacct cgtcggacta
      301 agcgtgacaa atcacattcc ctcgcagcat ccggtggtgc ttcgaacgca gcccaaaccg
      361 gccctgctgg ctgaggcggc tgagagaaat ggcgtcgagc cggcgctggt agaatccgtg
      421 atgcacgatg ccgcgcgcct cgacgcgccg gtgaatcggt agagcggcga accagccaac
      481 cgtggcgcga acaatcgcac ggcgcgcttg agaccggagc gagcacaggc aaccagcagt
      541 cggatcctgt ccgcaaaaag tcctaccggc cccgcgccgg ttttcgtttg gcttcttttg
      601 gcgccgcccg cccggtcttc ttcgatttcc tggccgcctt ggcccggctg gcaggcgtgg
      661 cctttttctt tcgaccggcc tttgacttct tcggcgatgt cttggacgac ttcttgggcg
      721 ccgctttctt ttttggcgaa gtttttgact ttttcggcgt tttgccggct ttcgatttgg
      781 cggatgatgt tccctgcttc gtgcgctgag gcggaatcgg ctggttcatc cagaaatcca
      841 gttcctgcgc gcgcgatccg cctggcatcc atccatcgag tggcttgaag atctgatcgg
      901 gatcgccgcc gctgccgaga aacgcgcgcc acgaataatg gtcgtaaacc ccgttgatgt
      961 gcagcgcgta ggcctgcgta agcttcttgt tgccgcggac gatcacgaga ttctcgtcgt
     1021 ttttctggga cgcggaatca gagaaattat gcgagccggt gatgacggcg cattggtccg
     1081 agaacggatc gatcacgatc gccttcgagt gaacgatcgc catcagatgc gccgcgtgca
     1141 tttcctggac gttgaactcg gtttcctccc aggacgggtg atcttttgcg ctgacgccgg
     1201 ccggaagccg aacatcgtcg tggaaggact gcgccggcgc gcccgacttt accacctggc
     1261 cgccgacggc gacgatatcg ccgttttgcg ttggcatgat cgtcgaaacc acgccacgaa
     1321 catagatatt gttctgcacg gcccgatcga gcaacgcgct cagcagcggc gactggcccg
     1381 gcatgaacat caaaaacagc gcgccttgct tggcgccggc gatcagatcg agagccggct
     1441 tgaattcagc ttcgccgttg gttgcggcga aatagaccga aatattgttg tgcgtcgtgg
     1501 ggttcgaatt tgacgcgatc agaccagccg gcagattatc gccggcggcg acgagtttgc
     1561 cccattggtc gagataacgg ctggcgataa cggtatcgtc aacaatgagc acgttgttca
     1621 attgcgtgca aaggccggtc gtcgtccagt tcgtgctgcc ggtcagcacg cggctggcgt
     1681 tgccatgcgc atcgctctcg accacgaatt tgttgtgcac gctcggtgac gattttccgt
     1741 gatgcgacag atcgcggtgc tttacctcga ggcctgccga tgagagctcc ggcgccaccg
     1801 tgggcctggt cgcactgccg ttgcccagaa gcaggtggcc gcgaccgcca aaaggtttga
     1861 gggcgtcgac gagttgttgg tcgttcattt catagatggc agcgtggatc gtgctgccgc
     1921 ggcgatcggc gtcgcgcagg aaggccagga tttcgtgcaa tgcgtcgccc gagagataaa
     1981 gcctggcctg aaaacccgag gtcgccaggt catccaggaa tttccgcagc gacgcatcgc
     2041 tgacatcgcc gttgacgaaa cggctgacga tttgtgacat cagcgttccg cgattgaaat
     2101 aggcctgcag cccgcctccg gcgtcgccgg aagcgatcag cggcgccgtc cagtccgtca
     2161 cggacgtcgc atcgggcgca aaagcgccat tcgccgcgct tgtcatcgcg gtgacgcgat
     2221 aggagacgtt atcggttgcg acgacgctat gatcggtcca gatgcagcgc cggatcggcg
     2281 attgcgccga tgagatgccg gtcggctgca ccggcccctc accggctttc ggcgggatgc
     2341 gattgaccag cacggtcgct gcctgcgtcg tgtcattgcg ccgttcgagc tggaaaccca
     2401 cccaggcgtc ggaccatgta tccggctgcc aggcaatcag cacatcgtcc gcgttggcat
     2461 aagccttgat agcaatcgtc atcatgccct ccctgcgctc aacttgaaca cgcttactcg
     2521 acgggtcgaa cgagttgcgg cggcgcaggt cgccataatt tcacagctaa agcgtgcacg
     2581 ccagcaagaa gccgcctatc gaaaacaccg aaacaacgaa cctcgaaaag ctgcgcggcg
     2641 gtggaccggc caagcgcccc tgactaagat cgcgtccatg tccgaaatcg aaaaaatcac
     2701 tacctctcct cgcttcacct tcgacgccct cgtgaccggc catcgcggtt cgccgttggt
     2761 gttgctgctg cacggctttg cggaatccat gaactgctgg cgcgcgcaga tggcggcgct
     2821 ggcggccgcg aattatcgcg cggtggcgcc gaaccagcgc ggttattcgc caggcgcgcg
     2881 gccggacacg ggtgacagcg ccaattatca tatcgaccgg ctgatggatg acgcgatggc
     2941 catcgttggc gcgtcaggtt atggcgaccg ccgctttcat ctggtcgggc acgactgggg
     3001 cggcagcatc gcctgggcgc tcgccgatcg ttttccggac cggctggcct cgctcaccgt
     3061 gctgtcgcgg ccgcatccga acgcgttcaa tcgcgcgctg cagatgcagg atggcgatca
     3121 ggcgcagcgg tcgcggcatc acaaggcctt tctcgaacct gacgccggcg agctgttgct
     3181 ggccgataat gcaaaatggc tccgcgagcg gctaagcgcg gccggcgttc cctcggccgc
     3241 gatcgaaaaa catctttccg tgctcggcaa caaggcagcg atggaggccg ccctcgcctg
     3301 gtatcgcgcg cgcggcgcga tccgctcgcc gcttggtccg atcaaggtat cgacactcta
     3361 catctggggc gatgccgacg acaccgtcgg gagggtggcc gccgagggca cgggagattt
     3421 catcaccgcg ccctatcgct tcgaggttct gcccggcgtc ggccatttcg ccgccgacca
     3481 ggtgccggat cgcgtcagcg agttattgct gcagcacgtg gcgacctatc cggcctgatg
     3541 gatcggccgc ggaacttcgc caactcgaaa atgtcccctc gatttggggc cgcgtcgctt
     3601 gatagtcgcg ttcaacgccg gcgcctgcgc cgcgatgaaa ctccacagcc ggtttgcgat
     3661 cgggccatgc ggcgcgtcac gccgcctcgc cgcaacgatc tcgccgccgc caccacgcag
     3721 atgttttccg gcgatcgaca gcaaacggcc atctcgcaac tcgtcggcga tcatgaagcc
     3781 tggcatgtgg ccccaaccca tgccctgcaa aatgatctcc ttcttcatct gctgatcgct
     3841 caccgtccat gtcctggcgc cctcgatcag gtaatagtcg cgggcgggcc accgcagcgc
     3901 cgtatcgcgg atgacgcatt gcacatgacc gcgcatttgc tcgggcgtga tccctttcga
     3961 aatgggaaac ggcaggaagt tcggcgccac gaccggaagc agcgtgccgg caaacagatc
     4021 gatgaattcc agccgcggat cgcttttgtc gatgtggtgg atgatgaggt ccgcctcatt
     4081 gtcgaacagc ctctcccacg ggcctgaaat cgcttcgaag tacagatgca gccgggtctc
     4141 ggggcagccc ttgaagaaat gacgcaagag tcccaggacc tccgggagcg gacagagatc
     4201 gccgagcacg atgcgaagct ccgcctcttc acccatcgcc agttggcgcg catgtttccc
     4261 cagcagggcg agatcagcca acaggccgcg cgcacggtcg tgaaaggacc tgccatgatc
     4321 ggtcagggtg acgcgatagc cgtctcggtc gagcaagcga agcccgagct ggctttccag
     4381 attcttgatt gacgtaaata ccgccgaatg ggttcggcgc agcttcgtcg cggcagcctg
     4441 aaatccgcct tcggtcacga cggcatcgaa gcagcgcaac tgattgatcg tgacgccgtt
     4501 cattgtcgat ttttccaaca aagaattgcg atattttgtt ctttttaaca tttccgccgg
     4561 gattagggta gcgccggctc aactttgccc gcgcatcccc cggaaggaga aggacggtga
     4621 tcaccctcca cggatttgga cggtttttcg gcacgccgga ttcgagcccg ttcgtcatca
     4681 aagtcatgat gcttttgaaa ttggcaggcg ttgcctatcg cgaggtgcag ggaaatcctt
     4741 tcacggctcc tcgacgcctc ctgccctacc tcagggatga ggatgtgacg gtagcgg
//
LOCUS       DASWGM010001049         9353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1245677,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001049 DASWGM010000000
VERSION     DASWGM010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9353
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245677"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1088
                     /locus_tag="VGA15_28230"
     CDS             114..1088
                     /locus_tag="VGA15_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX9591619.1"
                     /translation="MTEVAFPHKADSSAPPAHALRAGWFRRALDSDVFYSFRRSKLTM
                     AATTVTALFFLLAIFASLLAVQNPFDPAQLQLMNSRIAPLWTADGQSPFLLGTDEQGR
                     DVFSAILYGLRISLTVGVLGVVFSGALGIVLGLIAGYVGGAVDGLIMRIADVQLTFPA
                     ILIALLVNGVVKSVFGNRLDAMSTLAVLVFAIGLSFWVQYARTVRGSVMVEKNRDYVA
                     AAQLIGLPAPVIMLRHVLPNSMGPILVIATINLALAIISEATLSFLGAGMPDTMPSLG
                     TLIRIGNNYLFAGEWWIVAFPGFALAALIVSINLLGDWLRDALNPKLR"
     gene            1120..2106
                     /locus_tag="VGA15_28235"
     CDS             1120..2106
                     /locus_tag="VGA15_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591620.1"
                     /translation="MSVPVLSVRNLGVEFATRRSTLRAIDGISFDIAKGEVLGVVGES
                     GAGKSVTGLAVIGLIDPPGRLAGGEIYLSGLRIDNLPPEEMRRVRGKRIGMIFQDPLT
                     SLNPLYRIGDQLVETIRTHLNLSEQAARKRAIDLLAEVGIPAPEKRVDAYPHEFSGGM
                     RQRVVIALAICAEPELIIADEPTTALDVSVQAQIISLIKRLGRDHGTAVMLVTHDMGV
                     IAETSDRVAVMYAGRIAEIGPVQDVVQNPLHPYAKGLMGAIPTLAGDAARLVQIPGSM
                     PRLSAIPPGCSFNPRCAFAFDRCRVDRPEPIRHGAQAVACHLYDAAVKETAA"
     gene            2103..3104
                     /locus_tag="VGA15_28240"
     CDS             2103..3104
                     /locus_tag="VGA15_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX9591621.1"
                     /translation="MTSASFIDVRNLRREFDVSKPWLNRVLEGGHPEFLKAVDGVSFD
                     IAKGETFALVGESGSGKTTVARMVVGLLPPSSGEVIIDGVSMSDPRQLQARQQLRRRI
                     QMIFQDPYASLNPRFRVDAIVAEPIRAFDLIQGERNIQARVGELLGLVGLHADDGWKY
                     PHEFSGGQRQRVAIARALASESEFIVCDEPTSALDVSVQAQILNLMRDLQDKFGLTYL
                     FISHNLAVVRHMANRIGVMYLGRIVEIAEGRELFNNPRMPYTKMLLGAVPDLAMSGRQ
                     RIPVKGEIPNPISPPPGCAFNPRCPLAFDLCRRLAPALIDGVACHAVNNPAHTAVPA"
     gene            3208..5010
                     /locus_tag="VGA15_28245"
     CDS             3208..5010
                     /locus_tag="VGA15_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX9591622.1"
                     /translation="MSNINPDPFTTRPEIEGTFGVVTSTHWIATAVGMATLEKGGNAF
                     DAGVATAFTLQVVEPHLNGPGGDVPIIVHDVKRGRTEVICGQGPAPSGATIAHYRSEG
                     LEMVPGTGLLAACVPGTFESWMLLLRDYGTLRLRDVLEPAIAYARDGYPLVERASATI
                     ATVEQMFREHWTTSAAVYLPNGEVPKPGTLFTNKRLSETYARILQEAESAGTDRVAQI
                     ERARKAWSHGFVAEAIDQFCRTQEIMDVSGSPHRGVLRADDMARWQPSVEAPLTYDYG
                     RYTVCKPGVWSQGPVMLQQLALLKGFELDGLDPAGPEFIHLQIECAKLAFADREKFYG
                     DPKFTDVPIATLLSDAYNDQRRKLISEKASLDFIPGSVEGFGSVVKLRRQEGNREAVG
                     AMGAGEPTIGRFGEVRGDTVHFDIIDTAGNMISATPSGGWLQSSPVIPELGFCLGSRA
                     QMFWLDENHPAALAPGKRPRTTLSPSMALRDGEPYLAWGSPGGDQQDQWTTQFFLRHV
                     HAKMNLQEAIDAPAWHSEHFPISFWPRTARPGVLVVENRVPKATVATLKSRGHIVETG
                     PDWSEGRLTAASKVGRRRRAAANPRGMQGYAAGR"
     gene            5017..5727
                     /locus_tag="VGA15_28250"
     CDS             5017..5727
                     /locus_tag="VGA15_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX9591623.1"
                     /translation="MTWSIIARDNLTGQFGIAVATRFFAVGARVPHIAAGIGGIATQA
                     LVNPYYGIDGLKLLREGRKPRDIVETLIAADAGRESRQLHIMDAGGLVASHTGRDCVG
                     WCGHVEGKGFSIAGNMLAGARVLDDTAQAYASNEGRPFAQRLIAAMRAGEAAGGDKRG
                     KQSAALLIYGDEEWSDLDLRVDDHFDPLGELERLEGVSRERWVHFRNYLPTRKNPAGI
                     TDRETIDAGIEAAIAGKK"
     gene            5724..6713
                     /locus_tag="VGA15_28255"
     CDS             5724..6713
                     /locus_tag="VGA15_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX9591624.1"
                     /translation="MTAGPLIEIEGLRVTFHGDDGRTTHAVDRVDLSVANGATLGLVG
                     DSGCGKSVTSLAIMGLLSKHSAEVTGSIRFDGFDLLEVPDQTLRDLRGNRLAMIFQEP
                     MTSLNPSFTIGDQIIETILRHRGGSQRAARARAIELLRRVHIPSPERRIDEYPHKLSG
                     GMRQRVIIAMALACDPRLLIADEPTTALDVTLQAQILDLMRELKAASGAAIILITHDL
                     GVVAEVCDDVAVMYAGEIVERAAVDELFASPQHPYTVGLLGSIPRLGRRASHLATIEG
                     MVPNMANPPSGCRFAARCPFASDICVAAPPPMVNLSPGHWSRCLKAPLERLVS"
     gene            6889..8001
                     /locus_tag="VGA15_28260"
     CDS             6889..8001
                     /locus_tag="VGA15_28260"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX9591625.1"
                     /translation="MTALLEVQNLVKHFVADRSVFGRPTAFIKAVDGVSFSVDAGQTL
                     ALVGESGCGKSTVSRLVLRLIEPDSGHIRFEGRDLLALDANQLRAFRRDAQIIFQDPY
                     ASLNPRMTVSQILTEPLALHDLVPPSSRRDRVEELLRLVGLEPRFARRYPHEFSGGQR
                     QRIAIARALAVEPKLVICDEPVSALDVSIRSQILNLLRDLQDRLGLAYIFVSHDLAVV
                     KHIADRVAVMNLGVIVETADTQALFASPRHPYSRALLSAIPVPKPRAKRSRILLQGEM
                     PSALNPPAGCRFHTRCPYVIERCRVEPPELLADSAGHMTACHRTAQLPPPDAIVPADG
                     AFSPALERLIAAFSGPAEVPASSGVDIVGATPPEVV"
     gene            8026..>9353
                     /locus_tag="VGA15_28265"
     CDS             8026..>9353
                     /locus_tag="VGA15_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591626.1"
                     /translation="MKILHFAFTAAALWLSLGAGVHAQTTLRIGLAEDPDILDPSLAR
                     TYVGRIVFAAFCDKLFDIDEKLNIVPQLALSHETSADGKEVTIKLRPGVKFHDGEPFD
                     AEAAKFSLERHLTLPTSFRKPELAALDHVEIVDPLTIKLVLKTPYSPLLAQLTDRAGM
                     MISPKAAKEEGDKFGLHPVCAGPYKFVERVQQDRMVFEKFADYWNKDNIFIDRVVFLP
                     IVDSTVRLANLKSGGLDLIERLLATDIRDVRADPKLALSTALSLGYQGITINIGSDKS
                     KGPLSQSAKMRQALDLSIDREAINQVVYNGEFVPGNQWVNPDHPYYQKAYPVRSRDVA
                     KAKALLKEAGFTGPITVDFMVPKNVETETVAQVIQSMAAEAGFDMKIRVTETATALKA
                     AEAGEYQAFILDWSGRIDPDGNSYIFHKSKAPQNYSAYSNPDVDRLLDDAR"
BASE COUNT         1643 a         3083 c         2904 g         1723 t
ORIGIN      
        1 gatggtgtcc ctgatcttcg tcaccatcaa tctcgtggtc gatattctct acaccgtagt
       61 cgatccgcgc ctgcgctcga ccatcagccg cccggcctga gcgagagatc ttcatgaccg
      121 aagtcgcctt cccgcacaaa gccgattcaa gcgcgccgcc cgcgcacgcg ctgcgggcag
      181 gctggttcag gcgcgccctc gatagcgacg tgttttattc gtttcgtcgc tccaagctca
      241 cgatggccgc gaccaccgtc accgcgctgt tttttctgct cgccatcttc gcatcgctgc
      301 ttgccgtaca gaacccgttc gatccggcgc agcttcaatt gatgaattcg cgcatcgccc
      361 cgctatggac cgccgacggc caaagcccgt tcctgctcgg caccgacgaa caaggccgcg
      421 atgtgttttc cgccattctt tatggcttgc gcatttctct cacggttggc gttctcggcg
      481 tcgtgttctc cggcgcgctt ggcatcgttc ttggactgat cgccggttat gtcggcggcg
      541 ccgtcgatgg cctgatcatg cgtatagccg atgtgcagtt gaccttccct gctatcttga
      601 tcgcgctgct cgtcaacggc gtcgtcaaat cggtgttcgg caatcggctc gacgccatga
      661 gcacgctggc ggtgttggtg ttcgcgatcg gtttgagttt ctgggtgcaa tatgcgcgca
      721 ccgttcgcgg ctcggtgatg gtggagaaga acagggatta tgtggcggca gcgcagttga
      781 tcggactccc cgcccccgtg atcatgctgc gccacgtgct gcccaactcg atggggccga
      841 tcctggtgat cgccaccatc aacctcgcgc ttgcgatcat cagcgaggcg acgttgtcgt
      901 ttctgggagc cggcatgccc gacaccatgc cgtcgctcgg cacgctgatc cggatcggta
      961 acaactatct gtttgccggg gagtggtgga tcgtcgcctt tccgggcttt gcgctcgcgg
     1021 cgctgatcgt ctccatcaac ctgctcggcg actggctgcg cgacgcgctc aatccgaagc
     1081 tccgatgaga agcgccgcaa ctcgcgagta tttgatcgca tgagcgtacc cgtactctcc
     1141 gtgcgcaatc tcggggtgga gttcgccacc cgtcgcagca cgctgcgcgc catcgacgga
     1201 atttcctttg acatcgccaa gggcgaggtg ctgggcgtgg tcggcgaatc cggcgccgga
     1261 aaatccgtca cgggtctcgc cgtgatcggc ctgatcgatc cgccgggccg ccttgccggc
     1321 ggcgagatct atctgtcggg cctgcggatc gacaatctgc ccccggaaga aatgcgccgg
     1381 gtcaggggaa aacggatcgg catgatcttt caggatccgc tcaccagcct caatccgctg
     1441 taccggatcg gcgatcaact ggtcgagacc atcaggaccc acctcaatct ctcggaacaa
     1501 gctgcgcgca agcgcgccat cgatctgttg gccgaggtcg gcatccccgc ccctgaaaag
     1561 cgcgtcgacg cctatccgca cgaattctcc ggcggcatgc gccagcgtgt cgtgatcgcg
     1621 ctggcgattt gcgccgagcc cgagttgatc atcgccgacg agcccaccac cgcgctcgat
     1681 gtctccgtgc aggcgcagat catctcgctg atcaagcggc tggggcgcga tcacggcacc
     1741 gccgtgatgc tggtgaccca tgacatgggc gttatcgccg aaacctccga ccgcgtcgcg
     1801 gtgatgtatg ccggccggat cgccgaaatc gggccggtgc aggacgtcgt acagaacccg
     1861 ctgcatccct atgccaaggg cctgatgggc gcgatcccga cgctggcggg cgacgccgca
     1921 cgtctggtgc agattcccgg ctcgatgccg cggctttccg caatcccgcc gggctgttcg
     1981 ttcaatccgc gctgcgcgtt cgccttcgac cgctgccggg tcgaccggcc ggagccgatc
     2041 cggcatggcg cgcaagccgt ggcctgccat ctctacgacg ccgccgtgaa ggagactgcg
     2101 gcatgacttc ggcgtccttt atcgacgtca ggaatctgcg gcgcgaattc gatgtctcga
     2161 agccgtggct caaccgcgtg cttgaaggcg gccatccgga attcctgaaa gccgtcgacg
     2221 gtgtcagctt cgacatcgcc aagggcgaga cgtttgccct cgtcggggaa tccggctccg
     2281 gcaagaccac cgtcgcgcgc atggtcgtgg ggctgctgcc gccgagctca ggcgaagtca
     2341 tcatcgacgg cgtttcgatg agcgacccca ggcaattgca ggcgcgccag cagctgcgcc
     2401 gccgcatcca gatgattttc caggatccct atgcgagcct caatccgcgc tttcgggtcg
     2461 acgccatcgt cgccgaaccg atccgcgctt tcgacctgat ccagggcgaa cgcaacattc
     2521 aggcgcgggt cggcgagctg cttggtctgg tcggcctgca tgccgacgac ggctggaaat
     2581 atccgcacga attttccggc ggacagcgcc agagggtcgc gatcgcccgc gcgctggcgt
     2641 ccgaatccga gttcatcgtc tgcgacgagc cgacgtctgc gctcgacgtc tcggtgcagg
     2701 cgcagatcct caatctgatg cgcgacctgc aggacaagtt cggcctcacc tacctcttca
     2761 tcagccacaa cctcgccgtg gtccggcaca tggcgaaccg catcggcgta atgtatctcg
     2821 gccgcatcgt cgagatcgcc gaagggcgcg aactgttcaa taacccgcga atgccctaca
     2881 ccaaaatgct gctcggcgcg gtgcctgatc tggcgatgtc cggccgccag cggattcccg
     2941 tcaaaggcga aattcccaat ccgatcagcc cgccgcccgg ctgcgcgttc aacccgcgct
     3001 gcccgctggc gttcgatctg tgtcgcagac tggcaccggc cttgatcgac ggcgtcgcct
     3061 gccacgccgt taacaatccg gcccacaccg ccgtacccgc atgatcacga tgacgagagc
     3121 aaggcaccgc agtggacccg ttggcaggca gcctcggctg tggtcaattg cgcccgctgc
     3181 acggcagcgg cacaacgata ggtttcaatg agcaatatca atcccgatcc gttcacgacc
     3241 aggccggaaa tcgaaggcac ttttggggtt gtgacgtcca cccactggat cgccaccgcg
     3301 gtcgggatgg ccaccttgga aaaaggcggc aacgccttcg acgccggtgt cgccacggcg
     3361 tttacgctgc aggtggtcga gccgcatttg aacggtcccg gcggcgacgt gccgatcatc
     3421 gtgcatgatg tcaagcgcgg ccgcaccgag gtgatttgcg gccagggccc tgcgccgtca
     3481 ggcgccacca tcgcgcatta ccgcagcgaa ggcctggaga tggtgcccgg cacgggactc
     3541 ttggcggcct gcgttcccgg cacgttcgaa tcctggatgc tgctgctgcg cgactacggc
     3601 acgctgcggt tgcgcgacgt gctggagccg gccatcgcct acgcccgcga cggctacccg
     3661 ttggtggagc gcgcatccgc cacgatcgca accgtcgagc agatgttccg cgagcactgg
     3721 acgacgtcgg cggcggtcta tctgccaaat ggcgaagtgc caaaacccgg cacgctcttc
     3781 accaacaaaa gactttccga aacttatgcc cgtatcctgc aggaagccga gagcgcaggc
     3841 accgaccgcg tcgcacagat cgagcgggcg cggaaagcct ggtcgcacgg cttcgtcgcc
     3901 gaggcgatcg atcaattctg ccgcacgcag gagatcatgg acgtcagcgg ctcgccgcat
     3961 cgcggcgtgc tgagggccga cgacatggcg cgctggcagc catccgtcga agccccgctg
     4021 acttacgact atggccgcta caccgtctgc aagcccgggg tctggagcca gggcccggtg
     4081 atgctgcagc agctggcgct gttgaagggg ttcgaactcg acgggctcga tcccgcagga
     4141 ccggaattca ttcatctgca gatcgaatgc gccaaacttg cttttgccga ccgcgagaag
     4201 ttttatggcg acccgaaatt taccgacgtc ccgatcgcga cgctgttgtc cgacgcctac
     4261 aacgatcagc gccgcaaact gatctcggaa aaggcgtcgc tcgatttcat ccccggctcg
     4321 gtcgaaggat tcggttcggt ggtaaaactg cgccgccagg agggcaaccg cgaggcggtc
     4381 ggcgccatgg gcgccggcga accgaccatc ggccgcttcg gcgaggtgcg cggcgacacc
     4441 gtgcatttcg acatcatcga taccgccggc aacatgatct cggcgacgcc gtccggcggc
     4501 tggctgcaat cgtcgccggt gattccggaa ctcggcttct gcctcggcag ccgcgcgcag
     4561 atgttctggc tcgacgagaa tcaccccgca gcactggcac cgggcaagcg cccgcgcacc
     4621 acgctcagtc cgagcatggc gctgcgcgat ggcgagccgt atctggcatg gggttcgccc
     4681 ggcggcgatc agcaggatca atggaccacg caattctttt tgcggcacgt acacgccaag
     4741 atgaatctgc aggaagcgat cgacgcgccg gcatggcatt cggaacattt cccgatctcg
     4801 ttctggccac gcaccgcccg tcccggtgtg ctcgtggtcg agaaccgcgt gccgaaagcg
     4861 accgtcgcta cgctgaagag ccgcggccat atcgtcgaga ccggtcccga ctggtcggag
     4921 ggccgcctca ccgcggcctc gaaggtcggc cgccgccggc gtgctgccgc caatccccgc
     4981 ggcatgcagg gctatgcggc cggacgataa gccgagatga cctggtcaat catcgcccgc
     5041 gacaacctga ccggccaatt cggcatagcg gttgcgacac gttttttcgc ggtgggcgcg
     5101 cgggtgccgc acatcgccgc cggtatcggc ggtatcgcga cgcaggcgct ggtcaatccc
     5161 tattacggca tcgatggttt gaaattgttg cgcgagggac gcaagccacg cgacatcgtc
     5221 gagacgctga ttgcggcgga cgccggccgc gaaagccggc aattgcacat catggacgcc
     5281 ggcggacttg tcgcgtcgca taccgggcgc gactgcgtcg gctggtgcgg tcacgtcgaa
     5341 ggcaagggtt tttcgatcgc cggcaacatg ctcgcgggcg cccgcgtgct ggacgatacc
     5401 gcgcaggcct acgccagcaa cgaaggacgg ccgttcgcgc agcgcctgat cgcggcgatg
     5461 cgcgccggcg aagccgccgg cggcgataag cgcggcaagc agtcggcggc acttctgatc
     5521 tacggcgacg aggaatggtc tgatctcgac ctgcgcgtcg acgaccactt tgatccgctt
     5581 ggcgaactcg aacgcctcga aggggtcagc cgcgagcgct gggtgcattt ccgaaactat
     5641 ctgccgaccc gcaagaatcc ggccggaatt accgatcgcg agacgatcga cgccggcatc
     5701 gaagccgcga ttgcaggcaa gaaatgacgg caggcccgct gatcgagatc gagggcttgc
     5761 gggtcacctt ccacggcgac gacggccgca ccacccacgc ggtcgaccgc gtcgacctca
     5821 gcgtcgccaa tggcgcgacg ctcggcctgg tcggcgattc cggctgcggc aagagtgtca
     5881 cctcgctggc catcatgggc ctgctatcaa agcattcggc cgaagtcacc ggctcgatcc
     5941 ggttcgacgg gtttgatctg ctggaggtgc ccgaccagac cctgcgcgac ctgcgcggca
     6001 atcgcctggc gatgatcttc caggagccga tgacctcgct caatccgagt tttaccatcg
     6061 gcgatcagat catcgagacc atcctgcgcc atcgcggcgg ctcgcagcgc gccgcccgcg
     6121 cgcgcgcgat cgaactgctg cgccgcgtcc acatcccttc gccggagcgg cgcatcgacg
     6181 aatacccgca caagctctcg ggcggcatgc gccagcgcgt gataattgcg atggcgctgg
     6241 cctgtgatcc ccggctgctg atcgcggacg agccgaccac cgcgctcgac gtgaccttgc
     6301 aggcgcaaat cctcgatctg atgcgcgaat tgaaggcggc gagcggtgcg gcgatcatcc
     6361 tgatcacgca cgatctcggc gtggtcgccg aggtctgcga cgacgtcgcg gtgatgtatg
     6421 ccggcgagat tgtcgaacgt gccgcggtcg atgaattgtt cgcttctccg cagcatccct
     6481 acaccgtggg cctgctcggt tcgattccgc ggcttggtcg ccgcgcatcg catctggcca
     6541 ccatcgaggg catggtgccg aacatggcaa atccgccgtc cggctgccgc ttcgccgcgc
     6601 gttgtccttt cgccagcgac atctgcgtcg cagcaccccc accgatggtc aatttaagtc
     6661 ccggtcactg gtcgcgctgc ctcaaggcgc cgctggagcg gctggtgtca tgatggccga
     6721 gttctccgtt ccccgggcgc ggtgcagcac aagcgcagcg cagtggtgca ccgcagaacc
     6781 ggggcccggt gctggtgacg cgaagagtgg gtcccggttc tgcagcgcag cgttgcacgc
     6841 tgcgctgcgc ccgggacacg ctgactttcg cttttggagg acgagaaggt gaccgccctg
     6901 ctcgaagtcc agaatcttgt aaaacatttc gttgccgacc ggtcggtgtt cgggcggccg
     6961 acggcgttca tcaaggcggt cgatggggtc agcttcagcg tcgatgccgg ccagacgctg
     7021 gcgctggtcg gcgaatccgg ctgcggcaaa tccaccgtga gccggctggt gctgcggctg
     7081 atcgagccgg attcaggcca catccgcttc gagggccgtg acctgctcgc gttggacgcc
     7141 aaccaattac gcgcgttccg ccgtgacgcc cagatcatct tccaggatcc ctacgcatca
     7201 ctcaatccgc gcatgaccgt cagccagatc ctgaccgagc cgctggcgct gcatgatctg
     7261 gtgccaccct caagccgtcg cgaccgcgtc gaggaattgt tgcgcctggt cggactggag
     7321 ccgcgcttcg cgcggcgcta cccccacgaa ttttccggtg gccagcgcca gcgcatcgcc
     7381 attgcccgcg cactggcggt ggaaccgaaa cttgtcatct gcgacgagcc ggtatcggcg
     7441 ctggatgtgt cgatccgttc gcagatcctg aacctgctgc gcgatctgca ggaccggctc
     7501 ggcctcgctt acatcttcgt ctcgcatgac cttgccgtcg tcaagcacat cgccgaccgc
     7561 gtcgccgtga tgaatctcgg cgtaatcgtc gagaccgcgg atacgcaggc gctgttcgcc
     7621 tcgccacgtc acccttacag ccgcgcgctg ctgtccgcga tcccggtgcc taaaccgcgg
     7681 gcaaagcgca gccgcattct gctgcaaggc gagatgccga gcgcgctcaa tccgccggca
     7741 ggctgccgct ttcatacccg ctgcccctat gtgatcgagc gctgccgcgt tgagccgcca
     7801 gaactgctcg cagatagcgc aggacacatg accgcgtgtc accgcacggc gcaactgccg
     7861 ccgcctgacg ccatcgtccc tgccgatggc gccttttcgc cagcactgga gcgattgatc
     7921 gcggctttca gcgggcccgc ggaagtacct gcttcctccg gggttgatat agtcggggcg
     7981 acgccgccgg aagtcgtgtg aatcgtagat atggggatca gttcgatgaa aattttgcat
     8041 ttcgcgttta ctgcggcggc gttgtggctc tcgcttggag cgggcgtcca tgcccagact
     8101 acgctgcgca ttggccttgc cgaagacccg gacattctgg acccatcgct ggcgcgcacc
     8161 tatgtcggcc gcatcgtgtt cgcagccttc tgcgacaagc tgttcgacat cgacgaaaag
     8221 ctcaacatcg tgccgcagct cgcgctgtcg cacgagactt ccgccgacgg caaggaggtc
     8281 acgatcaagc tacggcccgg cgtcaaattc cacgacggcg agccgttcga cgccgaggct
     8341 gcgaaattct cgctcgagcg ccacctcacg ctgccgacct cgttccgaaa gccggaattg
     8401 gccgcgctcg accacgtcga aatcgtcgat cccctgacca tcaagctggt attgaagacg
     8461 ccgtattcgc cgctgctcgc gcagctcacc gatcgcgccg gaatgatgat ctcccccaag
     8521 gcggccaagg aggaaggcga caagttcggg ctgcatccgg tttgcgccgg gccctacaaa
     8581 tttgtcgaac gcgtgcagca ggatcgcatg gtgttcgaga aattcgccga ctactggaac
     8641 aaagataata tcttcatcga ccgcgtggtg tttctaccaa ttgtcgattc cactgtgcgg
     8701 ctcgccaatc tgaaatccgg cgggcttgac ctgatcgagc ggctgctggc gaccgatatc
     8761 agggacgtgc gcgccgatcc gaagctcgcg ctgtccaccg cgctttcgct ggggtatcag
     8821 ggcatcacca tcaatatcgg cagtgacaag agcaaggggc cgctcagcca gtccgccaag
     8881 atgcggcagg cgcttgacct ttcgatcgac cgggaggcga tcaatcaagt cgtctacaac
     8941 ggtgaattcg tgccgggcaa ccagtgggtc aatccggacc atccgtatta ccagaaggcc
     9001 tatccggtac gttcgcggga cgtcgccaag gccaaggcgc tgttgaagga agcgggattc
     9061 accggaccga ttactgtcga tttcatggtt ccgaagaacg tagagacgga aacggtcgcg
     9121 caggtgatcc agtcgatggc ggcggaagcg ggcttcgaca tgaagatcag ggtcaccgag
     9181 actgcgacgg cgctgaaagc ggccgaagcc ggcgaatatc aagccttcat cctcgactgg
     9241 agcggccgca tcgatcccga cggcaactct tatattttcc ataagtccaa ggcgccccag
     9301 aattatagcg cctattccaa cccggacgtc gacaggctgc tagacgacgc gcg
//
LOCUS       DASWGM010001050         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1507958,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001050 DASWGM010000000
VERSION     DASWGM010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507958"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..>1618)
                     /locus_tag="VGA15_28270"
     CDS             complement(107..>1618)
                     /locus_tag="VGA15_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591627.1"
                     /translation="DQGFEGNRFTGLTMYDALTMWDLSSADKPSVMIPGLATEWKVDD
                     ADKKKWTFKLRPGVIFHDGSPFNADAVVWNVDKVLKQDAPQFDASQVGVTATRMPTLA
                     SARKIDDMTVELITKEPDSFLPINLTNLFMASPSKWQKLFEAAEGADAKAKSQAAWAA
                     FAKDPSGTGPWKMSSFTPRERLELVKNENYWDKARVPKVDKMLLLPMPEANARTAALL
                     SGQVDWIEAPAPDAVAEIKQRGFVLTTNEEPHVWPWQFSRVEGSPWNDIRVRKAANLC
                     VDREGLKDGLLAGLMVPATGTYEPGHPWRGKPTFEIKYDKPAAQKLMQEAGYGPNKKL
                     AVKVQTSASGSGQMLPVPMNEYLQQALAECYFDVQLDVIEWNTLFTNWRRGVKDPTAN
                     GANATNVTYSAMDPYFSLVRFLQSSMAPPVSNNWGYINNPRFDELVTKARQTFDPAER
                     DQALAALHEASVDDAAFLYVAHDVAPRAMSPRIKGFVQPKSWVVDFSPISMVP"
BASE COUNT          286 a          518 c          492 g          322 t
ORIGIN      
        1 acccgtcttc gcggagttta tcatcgggcg gcgcttcgcg ccgacccgtt ggcgaagcca
       61 ccctctccca caaggggaga ggggaaggaa gcgtcccgtc aaccaatcac ggcaccatgg
      121 aaatcggcga gaagtccacg acccagctct tcggctgcac aaaacccttg atcctggggc
      181 tcatggcgcg gggcgcgacg tcgtgggcga cgtagagaaa cgccgcgtca tcgaccgagg
      241 cctcgtgcag tgcggcgagc gcctgatcgc gctcggccgg atcgaaggtc tggcgcgcct
      301 tggtgaccag ttcgtcgaac ctggggttgt tgatatagcc ccaattattc gataccggcg
      361 gtgccatcga cgattgcagg aagcgcacca gggagaagta cggatccatc gccgaatagg
      421 tcacgttggt cgcgttggcg ccattggcgg taggatcctt gacgccgcgg cgccaattcg
      481 tgaacagcgt attccattcg atgacgtcga gctggacgtc gaaatagcat tcggcgagcg
      541 cctgctgcag atattcgttc atcggcaccg gcagcatctg gccggagccc gacgccgagg
      601 tctgcacctt gaccgccagc ttcttgttcg gaccgtatcc cgcttcctgc atcagcttct
      661 gcgccgccgg cttgtcgtac ttgatttcga aggtcggctt gccgcgccag ggatgacccg
      721 gctcataggt gccggtcgcc ggcaccatca ggccggcgag caaaccgtct ttcaggccct
      781 cgcggtcgac gcagagattc gccgccttgc gaacgcgaat gtcgttccac ggcgagccct
      841 cgacgcgcga gaactgccat ggccagacat gcggctcctc gttggtcgtg agcacgaatc
      901 cgcgctgctt gatctccgcc accgcatccg gcgccggcgc ttcgatccag tcgacctgac
      961 cggagagcaa tgcggcggtg cgggcattgg cctcgggcat cggcagcagc agcatcttgt
     1021 cgaccttggg aacgcgcgcc ttgtcccaat aattctcgtt cttcactaac tccagccgct
     1081 cgcgcggcgt gaagctcgac attttccagg gaccggtgcc ggacgggtcc ttggcgaacg
     1141 cggcccaggc ggcttgcgat ttcgccttgg catccgcgcc ttcggcggcc tcaaacagtt
     1201 tttgccattt cgaaggactc gccatgaaca gattggtgag attgatcggc agaaaactgt
     1261 ccggctcctt ggtgatcaat tccaccgtca tgtcgtcgat cttgcgcgcc gaggccaggg
     1321 tcggcatccg cgtcgcggtc acgccgacct ggctcgcatc gaactgcggc gcatcctgct
     1381 tgagcacttt gtcgacattc cagaccacgg cgtcggcatt gaacggcgag ccgtcgtgaa
     1441 agatgacgcc ggggcgaagc ttgaacgtcc acttcttctt gtcggcatcg tcgaccttcc
     1501 attcggtcgc gagccccgga atcatcacgc tcggtttgtc ggcggatgac aggtcccaca
     1561 tcgtcaacgc gtcgtacatg gtcaggcccg taaaacggtt gccctcaaat ccctgatc
//
LOCUS       DASWGM010001051         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1508075,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001051 DASWGM010000000
VERSION     DASWGM010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508075"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1445
                     /gene="der"
                     /locus_tag="VGA15_28275"
     CDS             60..1445
                     /gene="der"
                     /locus_tag="VGA15_28275"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX9591628.1"
                     /translation="MSFTIAIIGRPNVGKSTLFNRLVGQKLALVDDEPGVTRDRREGE
                     GRLGDLEFTIIDTAGLDEGAKGSLTARMLEQTEAAIGLADALMFVVDARAGLTPNDRA
                     FADFARRANKPVVLVANKSEGKQGDAGAMEAYELGLGEPIQISAEHGEGLSDLYDALR
                     ALMPEPVEEPEEFDDDDVIASDEDIAKRPIRVAIVGRPNAGKSTLINHLLGEERLLTS
                     AEAGTTRDSISVEINWQGRDFRVFDTAGLRRRSRIEEKLEKLSVADALRAVRFAEVVV
                     LMMDAQNKFEEQDLRIADLIEREGRALVIAVNKWDLMGRQSSLIAGLRTDADHLLPQV
                     KGMPIVAVSGLMGEGIDRLMSAIEQAYAVWNRRVPTASLNRWFEQAVDNNPPPAVSGR
                     RLKLNYITQAKARPPSFILFCSRADAVPQSYLRYLVNSLREFFELPGTPVRISLREKA
                     NPFAHKRKRPS"
BASE COUNT          304 a          525 c          575 g          309 t
ORIGIN      
        1 gaggctcttg gcttttcatc tcattgacat gcgcttcata aagagcgcac ggatcgctta
       61 tgtccttcac gattgccatt atcggccggc ccaatgtcgg aaaatcgacg ctgttcaacc
      121 ggctggttgg acagaagctg gcgctggtcg atgacgaacc cggcgtcacg cgcgaccgcc
      181 gcgaaggcga ggggcgtctt ggcgatctcg aattcaccat catcgacacc gcgggcctcg
      241 acgagggcgc caagggctcg ctgaccgcgc ggatgctgga acagaccgag gcggcgatcg
      301 gcctcgccga cgcgctgatg ttcgtggtcg acgcgcgcgc gggcctcacg ccgaacgatc
      361 gcgcctttgc cgatttcgcg cgccgcgcca acaagccggt ggtgctggtc gccaacaaga
      421 gcgagggcaa gcagggcgac gccggggcca tggaagccta cgagctgggt cttggcgaac
      481 cgatccagat ttcagccgaa catggcgagg gcctcagcga cctctatgac gcgctgcgcg
      541 cgctgatgcc ggagcccgtc gaagaacccg aagagttcga cgacgacgac gtcatcgctt
      601 ccgatgagga tatcgcaaag cggccgatcc gggtggccat cgtcggccgc cccaacgccg
      661 gcaagtccac gctgatcaat catctgctcg gcgaggagcg gctgttgacc agcgccgagg
      721 ccggcaccac ccgcgattcg atttccgtcg agatcaactg gcagggccgg gattttcgtg
      781 tgttcgacac cgcggggctg cggcggcgct cgcggatcga ggagaagctg gaaaagctct
      841 cggtggcgga cgcgctgcgc gcggtgcgct tcgccgaagt cgtggtgctg atgatggacg
      901 cgcagaacaa gttcgaggaa caggacctgc gcatcgccga cctgatcgag cgcgagggcc
      961 gggcgctggt gatcgccgtc aacaaatggg atttgatggg acggcagtcg agcctgatcg
     1021 cgggcttgcg caccgacgcc gatcatctgc tgccgcaggt gaagggcatg ccgattgtcg
     1081 cggtttccgg tttgatgggc gagggcatcg accggctgat gagcgcgatc gaacaggctt
     1141 acgcggtgtg gaaccggcgg gtgccgacgg cttcgcttaa tcgctggttc gagcaggccg
     1201 tcgacaacaa cccgccgccg gcggtgtcgg gccggcggct gaaactgaat tacatcaccc
     1261 aggccaaggc gcggccgcca agtttcatat tgttctgttc gcgcgcggac gccgtgccgc
     1321 aatcctacct gcgctacctc gtcaactcct tgcgtgagtt tttcgagctt ccgggcacgc
     1381 cggtgcggat cagcctgcgc gaaaaggcca atccgttcgc ccacaagcgc aagcggccat
     1441 cgtgaacaat gcgtagacgg tctcccaact actgattggg cgtttggctg ggcgctaccg
     1501 ttgatcaccg gtattgctcc tcatctgagg cctggcaagt gaggcctggc aaggccgcga
     1561 tttcgtttgg caccaatccg ctgtcgcgta cgtccaagcg ggacgccaat gctgcgtgta
     1621 aaatcccgat cgtgccggaa gcttccgcgc tggatggcct gttggcgaac gctggcgtcg
     1681 ggcaggtgcg gggtcctttc gtgccgcgct tgc
//
LOCUS       DASWGM010001052         3441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1246793,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001052 DASWGM010000000
VERSION     DASWGM010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246793"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..701)
                     /locus_tag="VGA15_28280"
     CDS             complement(195..701)
                     /locus_tag="VGA15_28280"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX9591629.1"
                     /translation="MKPGFITALLALALSAGAAAAQSANSLKDRIVGSWNFVVAEVTA
                     PDGKKSFPFGETPKGILIFTADGRFAQIHVAGDVPRIASNNRLTGTPEEYAGIMQRSL
                     SVFGTYTVDEVKKTVTYNIVSASFPNWEGEAQTRVIDKLTADEFANTNPNVAGGRGSA
                     SNLYRRVK"
     gene            complement(1437..1877)
                     /locus_tag="VGA15_28285"
     CDS             complement(1437..1877)
                     /locus_tag="VGA15_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591630.1"
                     /translation="MKMSSAIRVALVALTAFAGATLSSASRADEGSVSLTIYKAGWII
                     GGSGGGGVLRFRGRSYALSTGGLDYGLVFGGSKTVLHGRVSNIYRPSDVAGVYGAAGA
                     GLAVGGGARAIVLTNQKGAVLELSGRQVGLMANADLSGLAITLK"
     gene            complement(2001..2546)
                     /locus_tag="VGA15_28290"
     CDS             complement(2001..2546)
                     /locus_tag="VGA15_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX9591631.1"
                     /translation="MNHDELVAAYTAPGRHYHNLKHIEDCLGALAGVKNLAAAEREIL
                     TEAIWWHDVVYDATRSDNEELSARLAEQHVRPDLGPEVGRLIRLTKTHQVASEDRLGA
                     ILVSIDLSILGAEPARYDAYAAAIRKEYAHVGDGDYRAGRAKVLRLFAARPFIFPDAD
                     FAARYDRQARQNLARELASLS"
     gene            complement(2611..3084)
                     /locus_tag="VGA15_28295"
     CDS             complement(2611..3084)
                     /locus_tag="VGA15_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591632.1"
                     /translation="MDRHLSEWKHSVELIWSAAAPDYGEAACLAAEISRASEETILRQ
                     AAAQALPILRSASTEDADRSTQDAARRRLGVIREVLHTLTTPRFGKRESAAKLPTPAE
                     CHRQLLGLPLGRRLSGAEIHRAYKRAAKSAHPDAGGTAGRFLELSAARDALMQER"
     gene            3202..>3441
                     /locus_tag="VGA15_28300"
     CDS             3202..>3441
                     /locus_tag="VGA15_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX9591633.1"
                     /translation="MPLLQNHIAVVTGAGSGIGRAIALGYAREGAQVVLLDMNQKSAA
                     EAAREIRDAGGKAESFALDVTQRENCVAVAKEIADK"
BASE COUNT          643 a         1074 c         1092 g          632 t
ORIGIN      
        1 tggccgtgca ggaaaagatg cggcagcagc gccactgcac cctctaaggc ccgcacaagg
       61 accgagatgc tgatagtcga tttcgttatt acaagggcgg attagccaac gggtcgcgcg
      121 cccgatgata aacgtcgcgt agtccgccat tccattgtca gccgttcggc caatctggcc
      181 tgcgccggcc gttcctactt cacccgccgg tacaaattgc tcgcgctgcc gcggcctccg
      241 gccacgttcg gattggtgtt ggcgaactcg tcggcggtga gcttgtcgat gacgcgggtc
      301 tgcgcctcgc cctcccaatt cgggaatgaa gcggacacga tgttgtaggt gaccgtcttc
      361 ttcacctcgt cgaccgtgta ggtgccgaat accgaaaggc tgcgctgcat gatacctgcg
      421 tattcctcag gcgtaccggt caggcggttg ttggacgcga tcctgggcac gtcgccggcg
      481 acatggatct gggcgaagcg gccgtcggcc gtgaagatca aaatgccttt gggcgtctcg
      541 ccgaacggaa acgacttctt gccgtcgggc gcggttacct cggcgaccac gaaattccag
      601 ctgccgacga tccggtcctt gagactgttc gccgattgcg cggcagcggc gccagcggac
      661 agggcaagcg ccagcaaggc ggtgatgaat cctggcttca taatatcttc cccggtcatc
      721 tgttggcgcg caagtcgtag caacgattag acgctttgcg tggaaaacct gatttgttga
      781 gccggatcaa ggtgatttgg cctgtccagt cctcgctcaa aaaagattcc gctttgccgc
      841 caccccaaat caaatctata tccctcgcat cccgtcccac acagaggggc gttacgcgaa
      901 cgtcacggac gtagggacgg gatgcgatgg acgcggcggc gcgcgagacg ggcgatgccg
      961 atgcggacgg cgaagtcgtg tggtcctgac gccccgacgc tggtgtcaag ttggcggaag
     1021 caattccgct gatgacggtg gcaaaagagc ccggtcaccg gggagagcgc gaaggaaacc
     1081 gttaaaacca ttgcgcgggg atgccgggcg ttttccggtg tgaccgtggt gactaacgcg
     1141 cgtgtttact accacaccac gcgcggctgc gggcgcatcg ggcgcccggc atccctgcgc
     1201 cctctgattg gagggcaagg aaatttgata acaagcctcg ggcgaattga gccgcgagat
     1261 cgcgaaggcg tgtctgtggg cacgcggtgt ttgaaaattg aatcctatat atttatcgcg
     1321 cacgcacgcg acgccgcggt ttcagtaggg cggattagcg aaagcgtaat ccgccatctt
     1381 tcttttcggc aacggcgggt tacggctgcg cctaacccgc cctacagacc cacccgctat
     1441 ttcaacgtaa tcgccagccc gctgagatcc gcattggcca tcagcccgac ctgccggccg
     1501 gacaattcca gcactgcgcc cttctggttg gtcagcacga tcgcgcgcgc gccgccgccg
     1561 accgcaagcc cggcgcccgc cgccccatag acaccggcga catccgacgg gcggtagatg
     1621 ttgctgaccc ggccatgcag cacggtcttc gatccgccga acacgaggcc gtagtccagc
     1681 ccgccggtcg acagcgcgta gctgcgccca cggaagcgca gcacgccgcc gccgccggag
     1741 ccgccgatga tccatcccgc cttgtagatt gtcagcgaca cggagccctc gtcggcacgg
     1801 gaggcggagg acagcgtggc gccggcgaaa gccgttagcg cgaccagcgc gacacgaatg
     1861 gcggatgaca tcttcattgg tacgtttccc tcgattttgt gagcaggggt tgcgacgcag
     1921 atcgaatctg cgctctggcg actgtagcaa gcccagcccg gatgagccaa cggattggca
     1981 aaagcccggc gacggaacca tcagctcaac gaagccagtt cgcgcgcgag gttttggcgg
     2041 gcctgccggt catatctcgc ggcgaagtcg gcgtcgggaa agatgaatgg ccgggcggcg
     2101 aacaggcgga gcacttttgc acggccggcg cgatagtcgc catccccgac atgagcgtat
     2161 tccttccgga tcgccgcggc ataggcgtca tatcgcgcgg gctccgcacc gaggatgctg
     2221 aggtcgatcg agaccaggat ggcgccgagg cgatcttctg atgcaacctg gtgtgtcttt
     2281 gtcaaccgga tcaggcggcc tacttccgga ccaagatcgg gacggacgtg ctgttcggcg
     2341 agccgggcgc tgagttcctc attgtccgac cgggtcgcat cgtaaaccac atcgtgccac
     2401 caaatggcct cggttagaat ttcgcgctcg gccgccgcaa gatttttcac gcccgcgagc
     2461 gcaccgaggc agtcctcgat gtgcttcagg ttgtgatagt gccggcccgg cgccgtgtaa
     2521 gcggcgacca actcatcgtg gttcatcgct ataggtatat ggcggaaacg acggaaatgg
     2581 tagacgcccc gttaatgccc ggccgcgttc tcacctctct tgcatcagcg catcgcgcgc
     2641 cgcggacagc tccagaaacc gccccgcagt gccgccggcg tccggatgcg ctgatttggc
     2701 ggcgcgtttg taggcccggt ggatttcggc gccggacaac cggcggccga gcggcagtcc
     2761 cagcagttgc cgatgacact ccgctggtgt cggcagcttc gcagcgctct cgcgcttgcc
     2821 gaatcgcggg gtggtgagcg tatgcaggac ttcgcgaatg acgccgaggc ggcgtcgcgc
     2881 ggcgtcctgg gtgctgcggt ccgcgtcctc ggtcgaggcg ctgcgcagga tcggcagcgc
     2941 ctgtgcggcc gcctgccgca gtatcgtctc ctcgctggct ctggaaattt cggccgcaag
     3001 gcacgcggct tcgccatagt cgggcgccgc cgccgaccag atcagctcaa cactgtgttt
     3061 ccattccgag aggtgtctat ccatggggca acgccagacc gaatgactac aatgcaaggg
     3121 taaagctttc cattttcgtt cccgccattt tgaattagtt tgtatcgaaa gctcaaaacc
     3181 aaaagcagag gaaccccgcc catgccactt ctccagaacc acatcgcggt cgtcaccggc
     3241 gcaggctcgg ggatcgggcg cgcgatcgcg ctcggttatg cccgcgaggg cgcgcaggtc
     3301 gtgctgctcg atatgaacca gaaatccgcg gccgaggcgg cgcgggaaat tcgcgatgcc
     3361 ggcggcaagg cggagagttt tgccctcgac gtgacccaac gcgaaaactg cgtcgccgtc
     3421 gccaaagaga tcgccgacaa g
//
LOCUS       DASWGM010001053         7474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_267360,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001053 DASWGM010000000
VERSION     DASWGM010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267360"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGA15_28305"
     CDS             complement(<1..414)
                     /locus_tag="VGA15_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963137.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX9591634.1"
                     /translation="MSLFAHYLSLPYLIEGIWFTLAVTALGLAGGLILGLVLAAMQLS
                     RFWMLAAFARGYTVIFRGTPLILQMVFAYDALPQIGLKLSAVGAAGLALAANEAPFIA
                     EMLRAGVLGVDRGQILAGQALGMTPGLLMRRIIAPQ"
     gene            complement(414..1253)
                     /locus_tag="VGA15_28310"
     CDS             complement(414..1253)
                     /locus_tag="VGA15_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX9591635.1"
                     /translation="MGIACLIVLAISGQAQAAPAGCAALQAKYPAWKGKTLVNAINPH
                     TPGYEAIDPKDPSKYVGFDIDLGERIGECLGFSLTYKAVTFAALLTTLSSGQADIVIS
                     DIYATEERAKAADFITYSKVFDGVLVAKGNPKKIDGINLSMCGSAAAENTGFVEVPLV
                     QALVPQCKAAGKAEPTMQLYDNNANCIQAILAGRADTYVNDVNTVDQAVKANPDKLEK
                     ALAVTIPYSVGIAVPKDKPEFRDAVMAALIEVQKAGIHMDLLKKWELDVNNFKEPDIL
                     SVK"
     gene            complement(1778..2143)
                     /locus_tag="VGA15_28315"
     CDS             complement(1778..2143)
                     /locus_tag="VGA15_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX9591636.1"
                     /translation="MIDVNAVASRYIDLWNERTPSRRREILAANWADDARYVDPLMSG
                     DGHDGVDALIAGVQQRFPDFRFQLIGQPNGFGNHIRFSWGLGPDGADSPIKGTDFAVV
                     QDGRIRSVTGFLDQMPQGA"
     gene            complement(2281..3195)
                     /locus_tag="VGA15_28320"
     CDS             complement(2281..3195)
                     /locus_tag="VGA15_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX9591637.1"
                     /translation="MRVPGIAIALAITCCAGPALSDTIGVNGVTRTFTAHLPDTKPAP
                     LVIVLHGNTQTGADMASRTSWLPVARRERFGVVFPDGLNRAWADFRPNANRAGRAPPK
                     DTDDVGFIAKLIEKFIADGTADPKHIYVTGLSNGGAMTMTMVCARAELFAAAASVIMN
                     LTDESASACHPSRAVSMLMMNGTADPLIPYQGGRGSSRFAAAGFWSTAKTLDFWRHAN
                     ACETQDAAAVDLDHRDHADRTTVTRISSRCQQGRDVVLYRINDGGHRMPGAFADARFP
                     RMVDGFLGPQNHDTDGAETIWGFFRQFP"
     gene            complement(3236..4894)
                     /locus_tag="VGA15_28325"
     CDS             complement(3236..4894)
                     /locus_tag="VGA15_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEX9591638.1"
                     /translation="MADDADVIVVGGGLAGLVAAAEIADAGKRVIVVDQEGEQSLGGQ
                     AFWSFGGLFLVDSPEQRRLGIKDSFDLALQDWMGSAGFDRDEDLWPKRWAEAYVAFAA
                     GEKRGWLRAMGHRIFPVVGWAERGGYDAMGHGNSVPRFHVSWGTGPGVVEPFERRARQ
                     AQANGRLTFKFRHRVDALSITDGAVDGVSGAILEADPVERGKSSSRKVVGDFTLRAQA
                     VIVASGGIGGNHDLVRQNWPKRLGEAPKNMISGVPEHVDGRMIGITEAAGARLINRDR
                     MWHYVEGIRNWSPIWPRHGIRILPGPSSMWFDATGTRLPSPLFPGSDTLGQLHYIMST
                     GYDYSWFVLTQSIIKKEFALSGSEQNPDLTGKSWLMTARRTTNKGAPAPVEAFKTHGV
                     DFIVRDNLADLIGAMNALSGDSLLKLEYVKAQIEARDREIENPYVKDTQVMNIHNARK
                     YIGDRLIRTAKPHRILDPLHGPLIAVKLNILTRKTLGGFETDLDSRVFGNDRKIMPGL
                     YAAGEAAGFGGGGMHGYRSLEGTFLGGCLFSGRNAGRAAAKAAG"
     gene            5206..5742
                     /locus_tag="VGA15_28330"
     CDS             5206..5742
                     /locus_tag="VGA15_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591639.1"
                     /translation="MKFVTGLLAAVLLLAGVGASHAVVRIAEDRGGRIGTYVDKYQGL
                     RSSGETVIIDGLCASACTIVLGAVPHDKICVTSHANLGFHAAWDFGANGRAVTNPEAT
                     QMLYSMYPSPVRRWISQRGGLTPHMIFLRGKQLMGMYKPCYLDAQASSPRPLSAHRDA
                     RQQDVIAPTPAALARQPD"
     gene            5818..6012
                     /locus_tag="VGA15_28335"
     CDS             5818..6012
                     /locus_tag="VGA15_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591640.1"
                     /translation="MAQPISTQQGMIAATQPSRRVAPVIVVAGAGFGAAVLLGALVLW
                     FHYGTAVFFEMLASGIGACF"
     gene            6035..6625
                     /locus_tag="VGA15_28340"
     CDS             6035..6625
                     /locus_tag="VGA15_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX9591641.1"
                     /translation="MDRTTRPLVIVAAFAGSLVVGLVLMLWTMGGLRSVAAPAAIGGP
                     FALTDQAGQTVTEKDLEGRPTLIFFGFTHCPDVCPTSLFEISEVLKAMGKDADRVNAY
                     FISVDPERDTDAAMKDYLSSFDPHLKGLTGNPDAVAKVIAGFRVYARKVPLKDGDYTM
                     DHTALIYLMDRDGKFVAPFNIKRTPGEAAADLKHYL"
     gene            6717..>7474
                     /locus_tag="VGA15_28345"
     CDS             6717..>7474
                     /locus_tag="VGA15_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX9591642.1"
                     /translation="MQSQIPLRANERLSRTIRTTLALSGLSLAAVLAVFLSGGAAQAQ
                     TLPKPGTEAAPQAVPGFWDPRRRPDRPDLSRLTVIRFLTETDYPPFNFTGPDGNPAGF
                     NVDLARQLCDEIKVSCTVQMRRFETLLDAIASNRGDAIIASLAVTPQLRARVDFTDPY
                     YRAPARFVSRRDAVMPEVRPEYLEGKKVGAIAGTSHEAYLKAMFTDAELHSYPNDDAL
                     RLALRRGEVDFIFGDAISLAFWINGTDSAECCAFS"
BASE COUNT         1357 a         2325 c         2326 g         1466 t
ORIGIN      
        1 ctgcggcgcg ataatgcgtc gcatcagcaa tccaggcgtc atcccgagcg cctgcccggc
       61 caggatctgt ccgcgatcga cgccaagcac gccggcgcga agcatctcgg cgatgaaggg
      121 cgcttcgttg gcggccagcg ccagaccggc ggccccgacc gcgctcaatt tgaggccgat
      181 ctgcggcaag gcatcatagg cgaacaccat ctgcaggatc agcggcgtgc cgcggaagat
      241 cacggtgtag ccgcgggcga atgcggccag catccagaag cgactgagct gcatcgccgc
      301 cagcaccaga ccgaggatca gcccgccggc cagcccgagc gcggtcaccg ccagggtgaa
      361 ccagatgccc tcgatcaggt acggcaggct gagatagtgg gcgaacagcg acattacttg
      421 acggaaagaa tgtcaggctc tttgaagttg ttcacatcga gctcccactt cttgagcagg
      481 tccatgtgaa tccctgcctt ctgaacttcg atcagcgcgg ccatcaccgc gtcgcggaat
      541 tcaggcttgt ccttgggaac ggcgatgccg accgaatagg gaatggtcac cgcgagcgcc
      601 ttctccagct tgtcgggatt ggccttcacc gcctgatcca ccgtgttgac gtcgttgacg
      661 taggtgtcgg cgcggcccgc gagaatggcc tggatgcaat tggcgttgtt gtcgtagagc
      721 tgcatcgtcg gctcggcctt gccggccgcc ttgcattgcg gaacaagcgc ctggaccagc
      781 ggcacttcga caaagccggt gttctcggcc gccgccgaac cgcacatcga taaattgatg
      841 ccgtcgatct tcttcggatt gcccttggcg accagaacgc cgtcgaacac tttcgaatag
      901 gtgatgaagt cggccgcctt ggcccgctct tccgtggcgt agatatcgga aatcacgata
      961 tcggcttgtc cgctcgacaa cgtggtcaag agggctgcga acgtcaccgc cttgtaggtc
     1021 agcgagaagc caaggcattc gccgatccgc tcgccgagat cgatgtcgaa accgacatat
     1081 ttgctgggat ctttcggatc gatcgcttca tagccgggcg tgtgcgggtt gatcgcgttg
     1141 acgagcgtct tgcccttcca tgccgggtat ttcgcctgca gcgccgcgca tccggccggc
     1201 gccgcctgcg cctgacccga aatcgcaagc acaatcaggc acgcaatgcc cattgcaaca
     1261 tttcggggtc gcttggccaa tctggctctg tcccggaaat ggcgcgcgtt cccaaaaaca
     1321 ttttcgagca tcatgagaaa aactcctctg ttgctgacga gcaggttggc tcggcaaagc
     1381 tgaacggtga ccgggaggct aggatgagca gagcgcgaat gcttgtctgc agccgcacaa
     1441 acacttgcag ccgccgctcc aatttttggg catcggtcgc acgctttatg ggctgaccgc
     1501 acaaatagcg atcattcgag gcggaaaagt ctcacctcgt ggaaactcga cggccctgaa
     1561 atgccgatcc gcaaagggtg agagcgctcg atcggtgttt agcgcccccc gctacggcgt
     1621 gacggtgagg gttgccttca cgtcatttgc gcggaatgcc gcggcaagct ggcagagcct
     1681 gcgattgttc gaccgcatgc tgatgcgaat ggtcatcgcc attccgcaag gaggacacga
     1741 ctgccccggg atggccgggg cggccgtact ttgttcgtca agcgccttgc ggcatctgat
     1801 cgagaaatcc ggtgacgctc cggatgcggc catcctggac gacggcgaag tccgtgccct
     1861 tgatcgggct gtcggcgcca tcggggccca gtccccatga gaaacggatg tggttgccaa
     1921 atccgttggg ctggccgatc agttgaaacc tgaaatccgg aaagcgctgc tgcacgccgg
     1981 cgatcagcgc gtcaacgccg tcgtggccat cgccgctcat cagcgggtcg acatatctgg
     2041 catcatcggc ccagttcgca gcaagaatct cgcgccggcg gcttggtgtt ctctcgttcc
     2101 acagatcgat atagcggctg gcaactgcgt tgacgtcgat cattctctct ctcctttcga
     2161 tattggctgg attgatcggc tgatcgacta tcgaaggttc gcgcgaccct atcgattacc
     2221 tcagaggtca tcgatttcga tttgctcata gccgttcgag cgaagccgaa aagctctgat
     2281 tcatggaaac tgccggaaaa atccccagat ggtttccgcg ccgtcggtgt cgtggttctg
     2341 cgggccgaga aacccgtcca ccatgcgcgg gaagcgcgcg tccgcgaaag cgccgggcat
     2401 gcgatggccg ccatcattga tgcgatagag cacgacgtcg cgcccctgct gacagcgcga
     2461 agagatccgc gtcaccgtgg tccggtccgc atgatcacga tggtcgagat cgaccgcggc
     2521 ggcgtcctgg gtttcgcaag cattggcgtg gcgccagaaa tcaagcgtct tcgccgtcga
     2581 ccagaagccc gcggccgcga agcggctgct gccgcgcccg ccttgatagg gaatcagggg
     2641 atcggcggtt ccgttcatca tcagcatgga gaccgctcgt gatggatggc aggcgctggc
     2701 cgattcgtcc gtgagattca tgatgacact ggccgctgcg gcgaacagtt cggcgcgtgc
     2761 gcaaaccatc gtcatggtca tggccccacc gttggagagg ccggtgacat agatgtgctt
     2821 cgggtcggca gtcccgtctg cgatgaattt ttcgatcagc tttgcgataa agccgacatc
     2881 gtcggtatcc tttggcggcg ctcggccggc gcggttggca ttggggcgga aatcggccca
     2941 ggcgcggttg agcccatcgg gaaagaccac gccgaaacgt tcgcgcctcg ccaccggcag
     3001 ccacgaggtt cggctcgcca tgtcggctcc ggtctgggtg ttgccgtgta gaacaatgac
     3061 aagcggcgcc ggcttggtgt cggggagatg agccgtgaac gtccgcgtca cgccgttgac
     3121 gccgattgtg tcggacaacg caggacccgc acaacacgtg atcgccaaag cgatggcaat
     3181 tcccggcacg cgcatcaact gtttcatcgc atcgatatcc gaaggtttag cttctttatc
     3241 ccgccgcctt cgccgccgcg cgcccggcat tgcggccgga aaacagacag ccgccgagaa
     3301 aagtgccttc cagcgaccga tagccgtgca tgccgccgcc gccgaagccg gccgcttcgc
     3361 cggcggcata aagccccggc atgatcttgc ggtcgttgcc gaacacgcgc gagtcgaggt
     3421 cggtctcgaa accgcccagc gtcttgcggg tcaggatgtt gagtttcacg gcgatcaagg
     3481 ggccgtggag gggatcgaga atccggtgcg gtttggcggt gcggatcagc cggtcgccga
     3541 tatattttcg cgcgttgtgg atgttcatca cctgcgtatc cttgacgtaa ggattttcga
     3601 tctcgcgatc gcgcgcctcg atctgggctt tgacgtattc gagcttcagc aggctgtcgc
     3661 cgctgagcgc attcatggcg ccgatgagat cggcaagatt atcgcgcacg atgaagtcga
     3721 cgccgtgggt cttgaatgct tccacggggg cgggcgcgcc cttgttggtg gtgcgcctgg
     3781 cggtcatcag ccagcttttt cccgtaagat cgggattctg ttccgagcct gagagcgcga
     3841 attccttctt gatgatgctc tgggtcagca cgaaccacga ataatcgtaa cccgtcgaca
     3901 tgatgtaatg cagctggccg agcgtgtcgg agccgggaaa cagcggcgag ggcagccgcg
     3961 tgcctgtggc gtcgaaccac atcgaagacg gaccgggcag gatgcggatg ccgtggcgcg
     4021 gccagatcgg cgaccagttc ctgatgccct cgacataatg ccacatccgg tcgcggttga
     4081 tcagccgcgc gcccgcggcc tcggtaatcc cgatcatcct tccatcgaca tgttcgggca
     4141 cgccggagat catgttctta ggagcctccc caagccgctt cggccagttc tgccggacca
     4201 ggtcgtgatt gccgccgatg ccgccggagg cgacgatcac agcctgggcg cgcagggtga
     4261 agtcgccgac gaccttgcgc gaactgcttt tgccgcgctc gacaggatca gcctcgagga
     4321 tcgcaccgct gacgccgtcg acggcgccgt ccgtgatgct aagcgcatcg acgcgatggc
     4381 ggaatttgaa cgtgagccgg ccgttcgcct gggcttgccg cgcccgccgt tcgaacggtt
     4441 cgacaacccc ggggccggtg ccccaactga catgaaaccg cggcaccgaa ttgccgtggc
     4501 ccatcgcatc gtagccgccg cgctcggccc agccgacgac gggaaagatt cgatggccca
     4561 tcgcgcggag ccagccgcgc ttctcgccgg ccgcgaacgc cacgtaagct tccgcccagc
     4621 gtttcggcca aaggtcttcg tcgcgatcaa aacccgccga tcccatccag tcctgcagcg
     4681 cgaggtcaaa actgtccttg atgccgagtc ggcgctgttc ggggctatcg accagaaaca
     4741 acccgccgaa cgaccagaag gcctgaccgc cgagactttg ttcgccctcc tgatcgacca
     4801 cgatgacgcg tttaccggcg tcggcaattt cggccgcggc gacgagacct gcgagaccgc
     4861 cgccgaccac gattacgtcc gcatcatcag ccatatcgcg tcgccccccg cttttgaccg
     4921 gattgaagcg cgcggttcga accacggtca accgcaaatg gttcttgcgt gtgtcttcgc
     4981 ggcggcggcg aaccggcaaa acttagggtt gttccagttt gggaccttgt cggttggcag
     5041 tgcaaccggc aagcaacact cgatttgaat ttgttttgat gcactcttcg gatctggaca
     5101 aaatcccgat ctcgcaacac gctggcgacg tcttgcatca ccaccgtctg gcatcactca
     5161 agacggttag gttcgggttc ggcaggtttg aactgaggct tggaaatgaa gttcgtgaca
     5221 gggttgctcg cggcggttct tctcctggca ggggtcggcg caagccatgc ggtggttcgg
     5281 atcgccgagg accgtggcgg acgcattgga acctatgtcg acaagtatca gggcctgcgc
     5341 agttcgggcg aaactgtgat tatcgacggg ttgtgcgcgt cagcctgcac tatcgttctg
     5401 ggcgcggttc ctcacgacaa gatctgcgtg acatctcatg ccaatctcgg cttccacgcc
     5461 gcctgggact ttggcgccaa tggccgcgcc gtgaccaatc cggaagccac ccagatgctg
     5521 tattcgatgt acccctcgcc ggtacggcgc tggatctcgc agcggggcgg actgacgccg
     5581 cacatgatct tcctccgcgg caagcagctg atgggcatgt acaaaccctg ctatctcgat
     5641 gcccaggcgt cctcgccgcg gcccctatcc gcgcaccgcg atgctcgtca gcaggacgtg
     5701 atcgctccga cccctgccgc gcttgcccgg cagcccgact agccctatct gaatatgaca
     5761 ggttggcgga cggcggcaac gccggccgtt gtcgcctgta cattcagatg gtcgcgcatg
     5821 gctcagccga tttccaccca gcaaggcatg atcgccgcaa cccagccttc gcgcagggtg
     5881 gcgccggtga tcgtcgtggc tggtgcaggt ttcggcgccg ccgttctgct cggcgcgctg
     5941 gtgctgtggt tccattatgg gacagcggtc ttcttcgaga tgctggcgtc cggcatcggc
     6001 gcctgcttct gacccggatc aagggaattt catgatggat cggaccaccc gcccactggt
     6061 catcgtcgca gcattcgccg gaagcctggt ggttgggctc gtgctgatgc tgtggaccat
     6121 gggcggattg cgaagtgtcg cggcacctgc cgccatcggc gggccgtttg cgctgaccga
     6181 ccaggccggt cagaccgtga ccgaaaagga ccttgagggt cgcccgacgc tgatcttttt
     6241 cggcttcacc cactgtcccg acgtgtgccc gacctcgctg ttcgagattt ccgaggtgct
     6301 caaagcgatg ggcaaggatg ccgatcgcgt taacgcttat ttcatctcgg tcgatcccga
     6361 gcgcgacacc gatgcggcga tgaaggatta tctgtcgagc ttcgatccgc atctgaaggg
     6421 tttgaccggt aatccggacg ccgtggcgaa ggtgattgcc ggtttccggg tctatgcacg
     6481 gaaagttccg ctgaaggacg gcgactacac catggaccac accgcgctga tctatctgat
     6541 ggatcgcgac ggcaagtttg tcgcgccgtt caacatcaag cggacgcccg gggaagccgc
     6601 ggccgatctg aagcattacc tctgaccagg aaccggatgc tgcgggtccc gaaacggaca
     6661 ttcgcgtctt ccgcaggatg gtctataagg ccgtccaccc cggaaaaccg tcggaaatgc
     6721 agtcacagat acctttgcgg gccaacgaaa gattgtctcg aacaatccgc acaaccttgg
     6781 cgctgagcgg attgtcgctc gcggctgtgc tggctgtgtt cctgtcaggc ggcgcggcgc
     6841 aggcgcaaac cctgccaaaa cccggtacgg aggcggcgcc gcaggcggtg cccggattct
     6901 gggacccgcg gcggcggccg gatcggcccg atctgtcccg cctgaccgtg atccgctttc
     6961 tgaccgaaac cgattatccg ccgttcaact tcaccggccc cgacggcaat cccgccggct
     7021 tcaatgtcga tctggcgcgg cagctttgtg acgagatcaa ggtcagctgc accgtccaga
     7081 tgcgccgctt cgagacgctg ctggatgcga tcgcgagtaa ccgcggcgac gccattatcg
     7141 cctcgctggc agtgacgccg cagctgcgcg cgcgcgtcga tttcaccgat ccgtattacc
     7201 gggcgccggc gcggttcgtg tcgcggcgcg acgccgtgat gccggaggtt cgtcccgaat
     7261 atctcgaggg caagaaagtc ggcgccatcg ccggtacctc gcatgaggcc tatctcaagg
     7321 cgatgttcac cgacgccgaa ctgcattcct atcccaatga cgacgcgctg cggctggcgt
     7381 tgcggcgcgg cgaggtggat ttcattttcg gcgacgcgat ctcgctggcg ttctggatca
     7441 acggcaccga ttccgccgaa tgctgcgcgt tctc
//
LOCUS       DASWGM010001054         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1247209,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001054 DASWGM010000000
VERSION     DASWGM010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247209"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2623
                     /locus_tag="VGA15_28350"
     CDS             <1..2623
                     /locus_tag="VGA15_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX9591643.1"
                     /translation="QLTFGGFLLVLAVIMITSTASVIAIRHIDATFAELQRLQSVGDL
                     AEDIDRRMNELRLAARDFVTDPGAQSSQVGGAAASLSEILKKTRLELAPEQQDMIDGV
                     TARLSTYRNGIERISGLISRRADLVAGLSPLREQFDAAIAETPDPVIAATLFQTQSRI
                     ASALLAHNPSAAERAAQSMRVLTISNPRLRATVDHYADAIVAISICERQIADIDREVL
                     GSEGRLIGQVTELLREVSARRGHVLSRDFARTLTEAKWQSIILGTSGVFFGLFAALFV
                     VRRTVRPLASIAASIRALAGGEKDTSIPATDVRNEIGDIARAAEVFRRTLVDADAARE
                     AAVHALAEQRLAEESYRKLFEGSVDGIYVTTLDGTLLNANPALARIMGYETPQDLIHG
                     IRDIADTVYIHAAARAQYESLMRRDGRVREFEYQVRALDGAILWLSDSATVVRNEAGE
                     AVRYEGTVRDITDQKRAEDAIAEGRRLLQQVIDTVPAVINVKDKQLRYVLMNRYMAGI
                     FGVEPADAIGRTTSDLMSRYGAQKTDENDKRVLAAGRGLGFYEEEYMDSSGNVRQWLV
                     NKLPLLDCDGEIENIVTVALDIGERKRGELEMRKAKDAAEAALRNLRETQNSLIEAEK
                     LAALGRLVAGVAHEVNNPVGISLTVASSLERKTAMFADEVARGNLKRSSLNEFLESSR
                     DASSQLVANLNRAAELIQSFKQVAADRNYSDQRIFDLGDLTEQVVMSLRPGLRKHRLA
                     LNVDCQPNLTMNSYPGPYGQVLTNLFLNSVAHAFPDGRPGAVDIQVRESGKDNVEIIF
                     SDNGCGMSLDVRRRAFDPFFTTRRDQGGTGLGLHIVYSIVTNRLGGRLDLDSEPGGGT
                     RIQMILPRVAPLEQAAE"
     gene            complement(2643..2990)
                     /locus_tag="VGA15_28355"
     CDS             complement(2643..2990)
                     /locus_tag="VGA15_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrlR family regulatory protein"
                     /protein_id="HEX9591644.1"
                     /translation="MKNGLYSIHVSLQDGRSGKGSGVVLFRDGKIFGGDAYLFYTGSY
                     TSKGDTFKGEVLVQRHTSSPDANPLFGAPSPVGIGVSGTFTDTAAVMDGTALVGKASQ
                     IFKATLRKLVEID"
     gene            3274..3696
                     /locus_tag="VGA15_28360"
     CDS             3274..3696
                     /locus_tag="VGA15_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591645.1"
                     /translation="MENQDLENKNPDHSHPRRAGRQKAPLTVGLAAALLALASHLGHA
                     RDRGQYANANPELKAWFESLKSGKGPCCSDADGTAVSDVDWQSYSGHYRVRLDGEWVD
                     VPDEAVITEPNRIGRTMVWPLRGYLGLTIRCFMPGSMT"
     gene            complement(3715..4098)
                     /locus_tag="VGA15_28365"
     CDS             complement(3715..4098)
                     /locus_tag="VGA15_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX9591646.1"
                     /translation="MPIVTWDHIHLRSPDPEATAAWLEDILGGVIIRGPGRIDVKLGG
                     ANVFIAPVTTGDGVNTPPVTPYQGLDHFGLVVKDIDAVAAEIKAKGVEFTKEPTTIRP
                     GVRICFIRGPQGISVELLERDRKYA"
     gene            4414..>4728
                     /locus_tag="VGA15_28370"
     CDS             4414..>4728
                     /locus_tag="VGA15_28370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591647.1"
                     /translation="MNTGLHGIEWTELAGLDGLARLARVSGDDFSDQPERVEARTFRR
                     GVVWLPAALMIGLGASWMLMSGSSSKTAKSEPARLVTAAAPQPPPSPATNIPQEGFPP
                     NPA"
BASE COUNT          879 a         1487 c         1522 g          840 t
ORIGIN      
        1 tcagctgaca ttcggcggct tcctgctggt gctggcggtg atcatgatca ccagtaccgc
       61 cagcgtcatc gccatccggc atatcgatgc gacgttcgcc gagctgcagc ggctgcagag
      121 cgtcggcgac ctcgccgagg atatcgatcg gcggatgaac gaattgcggc tcgccgcccg
      181 cgacttcgtg accgatccgg gcgcgcaatc cagccaggtc gggggggccg ccgcctcgct
      241 cagcgaaatc ctcaaaaaga cccggctgga gctcgccccc gagcagcagg acatgatcga
      301 cggcgtcacc gcgcggttgt cgacttatcg caacggtatc gagcgcattt cggggctgat
      361 ctcccgccgc gccgatttgg tcgccggcct atcgccgctg cgggagcaat tcgatgccgc
      421 cattgcggag acccccgacc cggtgatcgc ggcgacgttg tttcagaccc aaagccgcat
      481 tgcctcggcc ttgctggcgc ataatccgtc cgcagccgag cgggccgctc aaagcatgcg
      541 ggtgctgacg atatcaaatc ccaggcttcg cgcgaccgtc gaccattacg ccgacgcgat
      601 cgtcgcgata tcgatctgcg aacgtcagat cgccgatatc gacagggaag tgttaggctc
      661 ggagggacgc ctgatcgggc aagtcaccga attgttgcgt gaggtcagcg cgcggcgcgg
      721 ccacgtgctg tcccgtgatt tcgcgaggac cctgaccgag gctaaatggc aaagcatcat
      781 actgggcacc tccggcgtct ttttcggact ttttgcggca ctcttcgtgg tgcgacgcac
      841 cgtccgtccg ctcgcatcca tcgcagcctc gatccgtgcg ctggcgggag gcgagaagga
      901 cacatcgatt cccgccaccg acgtgcgcaa tgagatcggc gatattgcgc gtgccgccga
      961 agtattccgc cgcacgctgg tcgatgccga cgccgcgcgc gaggcggcgg tgcatgcttt
     1021 ggccgagcag cgcctcgccg aggagagcta tcgcaagctg ttcgaggggt cggtcgacgg
     1081 aatttacgtg acgacgctcg acggcacgct gctcaacgcc aacccggcgc tggcgcggat
     1141 catgggttat gagacgccgc aggatttgat ccacggcatc cgcgacattg cggacacggt
     1201 ttacattcat gcggcggcgc gcgcgcaata cgagtcgctg atgcggcgcg acgggagggt
     1261 tcgcgagttc gagtatcagg tgcgcgcgct cgacggtgcg attctgtggc tctccgacag
     1321 cgctaccgtg gttcgcaatg aggccggcga ggccgtccgc tatgaaggga cggtgcgcga
     1381 catcacggat cagaagcgcg ccgaggatgc catcgccgaa ggccggcggc tgctgcagca
     1441 ggtgatcgac accgtgccgg cggtgatcaa cgtcaaggac aaacagctgc gctacgtgct
     1501 gatgaaccgc tacatggccg gtatcttcgg cgtcgagccg gcggatgcga tcggccgcac
     1561 cacgagcgat ctgatgtcgc gctacggcgc gcaaaagacc gacgagaacg acaagcgggt
     1621 gctggcggcg ggccgggggc tcggattcta cgaagaggaa tatatggatt cctcgggcaa
     1681 tgtgcggcaa tggctggtga acaagcttcc gctgctcgac tgcgacggcg agatcgaaaa
     1741 tatcgtcacc gtcgcgctag acattggcga gcgcaagcgc ggcgagctgg agatgcgcaa
     1801 ggccaaggac gctgccgaag cggcgttgcg gaacttgcgc gagacccaga attcgctgat
     1861 cgaggcggaa aagctcgccg cactcgggcg gctggtggcg ggtgtcgccc acgaagtcaa
     1921 caacccggtc ggcatcagcc tgacggtcgc gtcgtcgctg gagcgcaaga cggcaatgtt
     1981 cgccgacgaa gtcgcgcgcg gcaatctcaa gcgctccagc ctgaatgaat tcctggagag
     2041 cagtcgcgat gcgtcctcgc agctggtggc caatctcaat cgcgcggccg aactgatcca
     2101 gtcgttcaag caggtcgccg ccgaccggaa ttattcggac cagcgcatct tcgacctcgg
     2161 cgatctgacc gagcaggtgg tgatgagcct gcggccgggc ctgcgaaagc accgtctggc
     2221 gctcaacgtc gactgccagc ccaatctcac catgaactca taccccggac cgtacggcca
     2281 ggtgcttacc aacctgttcc tcaattcggt ggcgcacgcc tttccggatg gcaggcccgg
     2341 cgccgtcgat atccaggtgc gggagtccgg caaggacaac gtcgaaatca ttttttccga
     2401 caatggctgc ggcatgtcgc tcgacgttcg gcggcgtgcg ttcgacccgt tcttcacgac
     2461 gcgtcgcgat cagggcggca ccgggctcgg cctgcatatc gtctacagca tcgtgaccaa
     2521 ccgtctcggc ggccggctcg atctcgattc cgagcccggc ggcggcacgc gaatccagat
     2581 gattcttcct cgggtcgcgc cgctggagca ggccgcggaa tagtgttgcc tctggctgcc
     2641 agctagtcga tctcgaccag cttgcgcaag gtcgccttga agatctggct ggccttgccg
     2701 accagagccg tcccgtccat gacagccgcc gtgtcggtga acgtgccgga gacgccgatg
     2761 ccgacggggc tgggcgcgcc gaacagcgga ttggcgtcgg ggctggaggt atggcgctgg
     2821 accagaacct cgcccttgaa ggtatccccc ttcgacgtat agctgccggt gtaaaacaga
     2881 taagcatcgc cgccgaagat tttgccatcc cggaacaaga ccacgccgct gcccttgccg
     2941 gaccgaccgt cctgcaggct gacgtggatc gaatacaaac cgtttttcat gatgtcccgg
     3001 ggttcggcat ccgtcaaagc ggtgtcgatg cgctgttatg ccaaagcagg gtacgcagcg
     3061 tcaacgcggc aggttggccg gtgcaaactc ttcgaatctc ggcgcgctgt atgacgtaaa
     3121 gatgacagga tggctcggcc agaaattggc ccgcgaaatg catagacgtc actgcactgg
     3181 ccggggtgct ggagcaggag acacgtgagc gactgggtcg attccggcgg cgacgcgccg
     3241 tatttctgta ccgacaacgg ggaactccaa gatctggaaa accaagatct ggaaaacaaa
     3301 aacccggacc attcgcaccc ccgccgcgcg gggcgccaga aagcgccgct gacggtcggc
     3361 cttgcggcgg cgctgctggc gctggcgtcc catctcgggc atgcgcgtga ccgcggccaa
     3421 tatgccaacg ccaaccccga actgaaagcg tggttcgaaa gcctgaagag cggcaagggg
     3481 ccctgttgct ccgatgccga cggcaccgcg gtcagtgacg tcgactggca atcctacagc
     3541 gggcactatc gtgtgcggct cgacggcgaa tgggtcgatg tccccgacga agccgtgatt
     3601 accgaaccga accgcatcgg ccgaaccatg gtatggccgt tgcgcggcta tctcgggctg
     3661 accatccgct gcttcatgcc gggcagcatg acgtagctcc gctcgcgttg gccctcacgc
     3721 atatttccgg tcgcgctcca gtagctcgac cgagatgccc tgcggcccgc ggatgaagca
     3781 gatacggacg cccggccgga ttgtcgtcgg ctccttggtg aattcgacgc ccttggcctt
     3841 gatctcggcg gccaccgcat cgatgtcctt caccaccaac ccgaaatgat cgagcccctg
     3901 atagggcgtc accggcggcg tgttgacgcc gtcaccggtc gtgaccggcg cgatgaacac
     3961 attggcgccg ccaagcttga cgtcgatccg cccgggcccg cggatgatca caccgccgag
     4021 gatgtcctca agccaggccg cggtcgcctc cggatccgga ctgcgcaaat ggatatgatc
     4081 ccaggtgacg atcggcatcg ttgctctccc cttgtttttc ttgcgagtcg cttgtggcca
     4141 cgctccacct cggctgcagc cgcgccaatt tagcggtagc gttcgcgcat ggccatcccc
     4201 tcgatccccc gacgggccca tctcgtcatg gcattccaag attgttgggt ttgaacgcaa
     4261 ccattttcgt cgtcattgat tgagtgtagg ataaaccggc ggaaccgcga aatcggcagt
     4321 cgtgtggaag gccgtgcggt gtaatgcccg cgcggtcttt ttacgatgcg gcgcaatagg
     4381 caaaatccgc ttcgcatgtc gatggcatga ccaatgaaca ccgggctgca tggtatcgaa
     4441 tggacagagc tagccgggct cgacgggctc gcgaggctag ccagggtcag cggcgacgat
     4501 ttttcggatc agcctgagag ggtggaggcc agaaccttca ggcggggagt tgtctggttg
     4561 ccggccgccc tgatgatcgg gctcggggcg tcgtggatgc tgatgtccgg ctcttccagc
     4621 aaaactgcca agagcgaacc ggcgcgcctg gtaacggccg cagcgccgca accgccgcca
     4681 tcgcccgcca cgaacatccc ccaggaagga ttcccgccga atccggcg
//
LOCUS       DASWGM010001055         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1508982,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001055 DASWGM010000000
VERSION     DASWGM010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508982"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGA15_28375"
     CDS             <1..336
                     /locus_tag="VGA15_28375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591648.1"
                     /translation="RGRSDRTRRAQRQRLTAADGFFASRCKARHARRKKVGGGCCGKT
                     IEAEPVFGQTQSIRGRRWTAQMEIIWNTTANNGISQKTTAVPRWSQSPCPSMAGFSDT
                     VFRGDTVGP"
     gene            complement(801..1217)
                     /locus_tag="VGA15_28380"
     CDS             complement(801..1217)
                     /locus_tag="VGA15_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591649.1"
                     /translation="MAAMMLMSVALVGILCVVAYTLAACALPLMLGFAAARFAYHTGS
                     GLIGASFIGLIAAAAAFGVFSVLFATLRSPILRLIVALVFAAPAGLAGYALVHGVTHE
                     AMPSAIWRQIFCFIGGVLVGVSARMRLAGSKTAATS"
     gene            complement(1438..>2136)
                     /locus_tag="VGA15_28385"
     CDS             complement(1438..>2136)
                     /locus_tag="VGA15_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEX9591650.1"
                     /translation="RTLGLRLALREQPEVGIVAVTHALSAQIFYRGADAHVLDIRPVS
                     TMLASHADGIEDTKAGKACADQHARWAAQMPRDVTDLWTFVVELDHDSRMALFAHCVA
                     LTVNAVKLPMDRRPRAMATADRLAEAVSLDMAAHWRPTARTYLGRVTKPHILAAVREA
                     VSDEAADRMADMKKQDMAEAAEQLLAGTGWLPALLRTPEPARLVGRQPEAPETADAEE
                     AARANDDGYPVAAE"
BASE COUNT          409 a          694 c          672 g          361 t
ORIGIN      
        1 cggggacgat cggaccgcac ccgaagggca cagcgccagc gcctgacggc cgccgacggc
       61 ttttttgctt cgcgatgcaa agcgcggcac gcgcggcgga aaaaagttgg cggcggctgt
      121 tgcgggaaga cgatcgaggc ggagccggtc ttcggtcaga cccaatccat tcgaggtcgc
      181 aggtggacgg cgcagatgga aattatctgg aatacgacag ctaacaacgg catttcgcaa
      241 aagacgacgg cggtaccgcg ttggtcacaa tcgccctgcc cctcaatggc agggttttcg
      301 gacaccgttt ttcgtggcga caccgtgggc ccctgacgcc gtaccttcgt gatgccatcg
      361 ttggcaatgc cgaagaagac cacccgtgaa acgcgatgcg atcggctcgg tcttaccggc
      421 gatcgcgcat ctggcgcttt atgctgcctg gctgtcgagg ccgccatggc atgccaccct
      481 catgagtctg aagcaggcga ttgaggccga accacgcaag gaactatgcg aagacggggc
      541 tggcgcgaga tcgccacagc cgccgctttc ttgcgttagc gattgaagcc gaatggcgga
      601 aacacgccgc aagcgtggtt ccggcgcagc cgaaagcgcg gcccgaaggg caacgcccta
      661 tcggaatgcc actcgctccc cgtccaaact ttttccgccc gtctctcaag ccaaaagagc
      721 ccatagcggg tgccttcccc accattcttg gcaagagcgt catcaagccg accagaaaga
      781 cgagcctcag tcgatctttc tcacgaagtt gcagctgttt tggagccggc caaccgcatc
      841 cgcgccgaga cgccgacgag caccccaccg atgaagcaga atatctgtcg ccagattgca
      901 gagggcatcg cttcgtgggt cacgccgtgg accagcgcgt aaccggccaa gccggcgggc
      961 gcggcgaaga ccagagccac aatgagacgc aggatcggcg accgcagcgt ggcaaacaga
     1021 accgaaaaca cgccgaacgc ggctgcagcg gcgatgaggc cgatgaagct cgcgccgatc
     1081 aggccggagc cggtgtgata ggcgaaacgc gcggcggcaa acccgagcat cagggggagt
     1141 gcgcaagcgg cgagtgtgta ggcgacgaca cagaggatac cgaccagcgc tacgctcatc
     1201 aacatcatgg cggcgatcat gatggtttcc ttaacgtggt agattgccct tcgttcggtc
     1261 gtgcgcatgt ccagtgacct tctcgtggcc gcgcacgttc acccctcgtc cctgagcgtc
     1321 ctgttttcca gctgagcggt cgaggtcatc cggccgcgcg ccgtcaggtg cgcggccgga
     1381 attttttcat gtcgtggcgg aaaagccggg accgctggcg cggtcccggc tcgtcgctca
     1441 ctcggcggcg acgggatagc cgtcgtcgtt cgcccttgcg gcctcctcgg catcggcagt
     1501 ctcaggcgct tccggctgcc gtcctaccag ccgcgcgggt tctggcgtcc gcaacagcgc
     1561 tggcaaccag ccggtcccgg cgagcaactg ctcggcggct tccgccatgt cctgcttctt
     1621 catgtctgcc atccgatcag cagcctcatc gctgacggct tcccggacgg cggcaagaat
     1681 atgcggcttg gtgacgcgac cgagataggt ccgcgcggtc ggtcgccaat gcgcggccat
     1741 gtcgagcgac acagcttccg ccaacctatc cgccgttgcc atcgcccggg gcctgcgatc
     1801 catcggcaac ttcactgcat tcaccgtcag cgccacacaa tgcgcgaaca gcgccatgcg
     1861 gctgtcatgg tcaagttcga ccacaaaggt ccacaggtcg gtcacgtcac gcggcatctg
     1921 cgccgcccaa cgggcgtgct gatccgcgca tgccttcccc gctttggtgt cctcgatacc
     1981 atccgcatgg gaagccagca ttgtgctgac cggacggatg tcgagaacgt gggcgtccgc
     2041 gccgcgatag aatatctgcg cagatagcgc atgggtgacg gccacgatgc cgacctccgg
     2101 ctgctcgcgc aaggcgaggc gaagcccgag ggtgcg
//
LOCUS       DASWGM010001056         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1512336,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001056 DASWGM010000000
VERSION     DASWGM010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512336"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VGA15_28390"
     CDS             <1..949
                     /locus_tag="VGA15_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEX9591651.1"
                     /translation="VLFFSSRGQVYKEKVWRLPVAAPNGRGKALINILSLEQGERITT
                     IMPLPEDESSWANLDVMFATTGGNVRRNKLSDFVDVRRSGIIAMKLDADEAIVDVQIC
                     TEHDDVLLTAAGGQCIRFAVTDVRVFQGRTSMGVRGIALPKDDKLISLTILRHMDADA
                     EERAAYLRRANAVRRGGVEEEAGTDSEDASGAIELGEQRYVEMSAHEQFVLTISENGY
                     GKRSSSFEYRTTGRGGKGIVAMSVNNRNGKLVASFPVEDSDQIMLVTDKGQLIRCPVE
                     GIRIAGRSTQGVIVFDTAEDEHVVSVEHIGEDAENGNGS"
     gene            complement(1028..2912)
                     /locus_tag="VGA15_28395"
                     /pseudo
     CDS             complement(1028..2912)
                     /locus_tag="VGA15_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
BASE COUNT          659 a          955 c         1009 g          526 t
ORIGIN      
        1 ggtgctgttc ttctcgtcgc gcggacaggt ctacaaggaa aaggtctggc gcctgccggt
       61 ggcggcgccg aacggacgcg gcaaggccct gatcaacatc ctgtcgctgg agcagggtga
      121 gcgcatcacc accatcatgc cgctgcccga ggatgaatcc tcctgggcca atctcgacgt
      181 gatgttcgcc accaccggcg gcaatgtccg ccgcaacaag ctgtcggact tcgtcgacgt
      241 ccgccgttcc ggcatcatcg cgatgaagct cgacgccgac gaggcgatcg tcgatgtgca
      301 gatctgcacc gagcatgacg acgtgctgct gacggcggcc ggcggccagt gcatccgctt
      361 cgccgtcacc gacgtgcggg tattccaggg ccgcacctcg atgggcgtgc gcggcatcgc
      421 tttgccgaaa gacgacaagc tgatctcgct gaccattctg cgccacatgg acgccgatgc
      481 cgaggagcgc gccgcctatc tgcgtcgcgc caatgcggtg cggcgcggcg gcgtcgagga
      541 ggaggcgggg accgacagcg aggacgcctc cggcgctatc gaacttggcg agcagcgcta
      601 tgtcgaaatg tccgcccatg agcaattcgt gctgaccatc agcgagaacg gctatggcaa
      661 gcgctcgtcg tcgttcgaat accggaccac cggccgcggc ggcaaaggca tcgtggcgat
      721 gtcggtcaac aaccgcaacg gcaagctggt ggcgtcattc ccggtcgagg acagtgacca
      781 gatcatgctg gtgaccgaca aggggcaatt gatccgctgt ccggtcgagg gcatccggat
      841 tgccggccgc tccacccagg gcgtcatcgt gttcgatacc gccgaggacg agcatgtggt
      901 gtccgtcgag cacatcggcg aggacgcaga gaacggcaac ggaagttaaa gaaacgaata
      961 gcgaatggcg agtagcgaat agaaagttcg ccttactatt cgctactact attcgccact
     1021 cgccctctca aatctcgatc tcggtcccga actcgacgac ctgacgggtt ggcacgccga
     1081 agaacgcagc ggagcgttcg gcattgcgtt gcaggaacgc gaacacgttt tcccgccaca
     1141 cccacatgcc cgggacatct tcgctcggga tgatggtctc gcggccaatg taataggtga
     1201 tatcggtgag atcgatgccg ggcagcttgc cctgctcgca ggcgagtttt aatccttcga
     1261 aaatcgttgg atattgcatg aaaccatagt gcaggattac gcgggtaatg ccctcgatca
     1321 cctcgataat ctccgcccga tcttcatcgg caatgcgcgg ggcttcctcg atcagcacgg
     1381 taacgagcac cacgcgctgg tgcagaacgt ggttgtgctt gacgaattcc gtcagtgcca
     1441 gcgggacgcc gttcggggca gacgccagaa acaccgcggt acctgaaagt ctggcgtggc
     1501 atctgttgac ggcggtctcg atcagatctt cttccggctg acgcagcttg ccgcgcgccc
     1561 gttcgaccag cttgacgccg cttcgccagg tcaacatcag gaaagcgatc acggccgcga
     1621 gcaacagcgg aaaccacccg ccctcgaaca gtttggtcga attcgccgcg aagaagatga
     1681 cgtcgatgac aaagaagaag ccgttgaccg cgattacgat gacgggcgaa tagccccact
     1741 ggatcgccac cagagcggcc agcaatgtcg tgatcgccat caacagcgac acggcaattc
     1801 cgtaagcgcc ggcaagcgcg tccgatgtgc cgaagccgat gacggcgccg agtgtcgccg
     1861 ccgccagcaa ccagttgacc agcggcacat agatctgacc catcgcgtgg ctggtggtgt
     1921 gtttgatctg catgcgcggc aggaagccga gctggatcga ctgctgcgtc agtgaaaata
     1981 ccccagagat gatagcctgt gaggcaatca cggtggccac ggtggcgaag gcgacgagcg
     2041 gatagtgcag ccagtcaggt gcgagctgat agaagggatg gtcgagcgcg ctggcgtcgg
     2101 tgatcagcag tgctgcctgc ccgaagtaat tgaggacgag ggcagggagt gccacgatga
     2161 accatgcgag ccggatcggt acccggccga aatggcccat atcggcatac atcgcctcgc
     2221 ctccggtcac cgccagaaag gcggcgccaa ggatggcgaa gctgatgtgg aagtcctcgc
     2281 gtatcaataa atcgaaagca taaagcggac ttagcgcggc aagcacgccg ggcgcccgca
     2341 cgatgccgtg gattccgagc agcgcaatca ccacgaacca ggcgagcatg accggcccga
     2401 agatattgcc gataaaacct gcgccctttt tctgaacgat gaaaagcccg atcaggatgg
     2461 cgatggtcag cggcaccacc gcgggcgcca gcgaaggcgc atcgaccttc aagccctcga
     2521 tcgcgctcag caccgagatc gccggcgtga tggcgccgtc gccgtagagc agcgctgccg
     2581 acaaggccga cgaccaggag ctgcgcccgc cacgtccccg gccgggcatt gcgggcggaa
     2641 agcagcgcca gcagcgccac gatgccgcct tcaccgcgat tgtcagcgcg caggatcagc
     2701 agcgcatatt tcagcgaaat gatcaggatc agcgcccaca ggatcagcga taccacgccg
     2761 aggatcgctt caggggcaag cggcccgcca tgggcggccg ctttcgcggc ttccttcagc
     2821 gcgtagaggg gactggtacc gatatcgcca tagccgacgc cgagcgcgcc gattgttgcc
     2881 gcccaaggaa gcgtgccgga accgggtgga atggcggcgg aaacggcagt cactggcgga
     2941 gcccccaagc ggtttcaagg gtatccaagg gtgtcgctcc cgtcggctag aggggcacga
     3001 tcacatgcca gtctgcgacg cggcgccaga aattactatg agatttgtct ggttccggaa
     3061 agcagtcgcg gttagggcgt gcggtcggcg gttaccagcc gggcttcctt gatggtcacg
     3121 gtctttttgg cggctgcgcg caggcagga
//
LOCUS       DASWGM010001057         8950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_271300,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001057 DASWGM010000000
VERSION     DASWGM010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271300"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..2009
                     /locus_tag="VGA15_28400"
     CDS             3..2009
                     /locus_tag="VGA15_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX9591652.1"
                     /translation="MTTILLTAAASSLLWWRTAEATSRQLASTINEQIVAAVRKEVSA
                     IVGEARAAHTAIRTLFLQNVLDTREADKREFVFLSQLQSHATISWVAFGWPDGSFFAA
                     HKLGDGHLEMMEISLTDHPGQRRIDEYAVIPGDIEFEQRRFVPTDFRVVDQVWFKTGM
                     GAKGPQWFKVLDHPIGQRPSIAFAGPIDVYQDRQGVLAIIIEYTRFAHFLAQLEVGRT
                     GTAFIFDGSGELIAAPDKEADELHPARSQEALLPLARMVLAQAGDEDRKEAWRRRLTS
                     QGEAYEVALTPLPFPGWSLATVIPEAEFLGPVETTLRRLIIGLAAGALLAALLSAMLV
                     RSVIAAPLARVVGEIRHVESFALDKVRRHPSRLAEISSLSGAIAEMAAGLSAFRKFIP
                     ADLVRSLLSQGVEAKPGGSIQELTVMFVDVAGFTGLSERMGDRVVLLLSRYLDAVSEV
                     IVANGGTIDKFIGDAVMAFWGAPTAQQDHAVRCCRAALACRRAIEAAGVNDDQGHPLQ
                     IRIGINSGRMLVGNIGSELRLNYTVIGDAVNVASRLESANKQYGTLILIGAETRRLIR
                     DAFIIREIDSIVVYGRTEGLAVYELIGLAEVLGENTAWIASYEEGLSRYRRRDFSGAI
                     THFEAVLSGRPHDWPASLLLERCKCLQQSGVDEEWSPVAALKAK"
     gene            complement(2180..2467)
                     /locus_tag="VGA15_28405"
     CDS             complement(2180..2467)
                     /locus_tag="VGA15_28405"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX9591653.1"
                     /translation="MSNESGAQLSEPRDLAHHNIGQLNRAMVGWFEFARKTLDAAEAN
                     VAALCNHASQLAQAESPVECVRLQTEFVKSSMASMQKQSTDIMSAGRKAAE"
     gene            2598..3536
                     /locus_tag="VGA15_28410"
     CDS             2598..3536
                     /locus_tag="VGA15_28410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9591654.1"
                     /translation="MEIHQVRYFLAVCEHLSFTQAARQCHVTQPSLSRAIQHLEKEFG
                     GYLFDRRRSKVRLTELGKLVQPYLQEAWEQAQAAKKEAKDYSSKAPTQLNLAIMCTIA
                     PRLLIQMFSRFRSARPDINLDLIDGTAQSIEEQLIGSKAEAAIYCRPDRALDPRLNYL
                     PLFREQMMIVLPAEHRLAARETIEIADLAGERYVQRSFCEFNDMVDRVFDDRGIDCET
                     VYRSDRDDWVLAMIASGFGFGFFPRYSISNAAVVARPLVNPEFWREVSLTTVKGRPYS
                     RAVGALVHEAMRSAWLDEDALAVRNHPPASEDPDHA"
     gene            complement(3631..3852)
                     /locus_tag="VGA15_28415"
     CDS             complement(3631..3852)
                     /locus_tag="VGA15_28415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591655.1"
                     /translation="MLAAEQQAAELRFEEFDGARQRRLGNMAEFGGAREIALGADGQE
                     ISDLVHFHKAIPLARMWPIARKGPPSPIV"
     gene            3832..4620
                     /locus_tag="VGA15_28420"
     CDS             3832..4620
                     /locus_tag="VGA15_28420"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEX9591656.1"
                     /translation="MLFRREHPHSHLTELGEIVRPHLQQVWEQSYAAITQAHEFTAVG
                     RSRLKIGIMCTIAPALLTDLLMRMRIRHKAIEVEIVDGSARDLEEQLVGGHIAAAVYG
                     KPDKTDSCVARIPLFRERMMIAMPRDHRLAGHPSIRFADLAGVPQIRRSRCEYNELVA
                     RLFRQHRIDCEIAFDSPRDDWAQALIAGGLGFALMPQHSIDHPGVLSRPLIEPTLWRE
                     ASLFTCRERTNGDGLDALVQEATRMTTASARVPAAGNSLADDAR"
     gene            complement(4659..5804)
                     /locus_tag="VGA15_28425"
     CDS             complement(4659..5804)
                     /locus_tag="VGA15_28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX9591657.1"
                     /translation="MQDVVGRGAKVNDLPGQVVLVLQGGGALGSYQAGVYQALHEAGI
                     EPDWIIGTSIGAINASLIAGNEPRARLSRLQEFWKRMEQSPAWNFGSVFPGFNEKLSY
                     WSTITHGVAGFFRPNPLAHAGDAYVLGPERAGYYTTTPLQKTLEELVDFDLVNRCKPR
                     LTVGAAHVRTSQMRYFDSRDGTITVKHVMASGALPPAFPAVRIEGELYWDGGILSNTP
                     TEAVFDDDPRKDSLIFSVHLWNPAGSEPTTMAEVFNRQKDVQYSSRIASQIVRQQQIH
                     RLRHVINQLVTHLPEAERSSAAARELASYGCQTRMHVVRLLAPQLDRETHTKDIDFSP
                     DGIKQRWDAGYTHTRSVLARSPWVGEFDPLSGIVFHEHMELMPQAAE"
     gene            complement(5884..6708)
                     /locus_tag="VGA15_28430"
     CDS             complement(5884..6708)
                     /locus_tag="VGA15_28430"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX9591658.1"
                     /translation="MNIPVKPQTSNARRALDGKVALVTGSTSGIGLGIVQALAAVGAE
                     VVLNGLGSAAEIGRTREQIAAEFGVKASFSPADMTRPKSIAEMIAATVAQFGRLDILV
                     NNAGIQHVAPLDQFPAEKWDQILAINLSSAFHTTRLALPAMRQNGFGRIINIASAHGL
                     VASPFKAAYVAAKHGIVGLTKVTALETAEEGITCNAICPGYVYTPLVEAQIDSQAKAH
                     GISRDQVIRDVLLAQQPNKRFATVEELGALTVFLSTEAAASITGVALPVDGGWTAH"
     gene            6901..7797
                     /locus_tag="VGA15_28435"
     CDS             6901..7797
                     /locus_tag="VGA15_28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX9591659.1"
                     /translation="MEMHQVRYFLAVAQLLNFTRAAEECHVTQPSLTRAIKQLEAELG
                     GDLFRRERPTQMTELGQRMHPLLKQCYEAAIGARSLASSFKSGEIGALWIALTHSVDL
                     SLLIPFLNEVKRQFNRLEFKFLRGKSGEVAEMLKKGEAELGIAAEIDQDWDRLDTWPL
                     FTEDFRLVVSRQHRLAERDSVELEDLRAEQLLSRNYCEHAERLGASLREHGLNVDRGH
                     EVSSERDLIELVEADIGVAIIPDTSPIPQTLKRTAVDGLDARRTVHLYGVAGRERTAV
                     ASAIMRMLRGADWRPSKRDVGM"
     gene            complement(7827..8066)
                     /locus_tag="VGA15_28440"
     CDS             complement(7827..8066)
                     /locus_tag="VGA15_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEX9591660.1"
                     /translation="MLAIPRRYSHFVFGVIQSGLTCAIAAAIASFPFIETGGFIQNWL
                     LSWLISWMTMLPLVLVAAPAIRSLSILLTREEPAN"
     gene            complement(8197..8661)
                     /locus_tag="VGA15_28445"
                     /pseudo
     CDS             complement(8197..8661)
                     /locus_tag="VGA15_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
     gene            complement(8693..>8950)
                     /locus_tag="VGA15_28450"
     CDS             complement(8693..>8950)
                     /locus_tag="VGA15_28450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9591661.1"
                     /translation="LRSNRVNDLSCVIADGQMPGMSSVELQAYLLTQGYRVPFIFFTA
                     FPDERIRAQAVRSGAICYLTKPFDGDSLVQGLQAALKKHDS"
BASE COUNT         1709 a         2788 c         2725 g         1728 t
ORIGIN      
        1 tgttgacgac gatcctgttg acggctgcgg catcaagcct gttgtggtgg cggacggcgg
       61 aagccaccag ccggcaactc gccagcacca tcaacgagca gatcgtggcc gccgtccgca
      121 aggaggtgtc ggcgatcgtc ggcgaagcgc gcgccgctca tacagccatt cgcaccctgt
      181 tcctgcagaa cgtgctcgac acccgcgaag ccgacaagcg cgagttcgta ttcctgtcgc
      241 aacttcaatc gcatgcgacg atctcctggg tcgccttcgg ctggcccgac ggctcatttt
      301 tcgccgcgca caagctcggc gacggccatc tggagatgat ggagatctcg ctgaccgatc
      361 atcccggcca gcgccggatc gacgaatatg cggtcattcc aggcgacatc gaattcgaac
      421 aacgccggtt cgtgccgacc gattttcgcg tcgtcgacca ggtttggttc aaaaccggca
      481 tgggggccaa gggaccgcaa tggttcaagg ttctcgacca tccgatcggg caacgtccgt
      541 cgatcgcgtt tgcgggtccg atcgacgttt accaggaccg gcaaggcgtc ctcgcgatca
      601 tcatcgaata taccaggttc gctcattttc tggcccagct cgaggtcgga cgcaccggaa
      661 ccgcgtttat cttcgacggc agcggagagc tgatcgcggc tcccgacaag gaggccgacg
      721 aactgcatcc cgcgcgtagt caggaagcgc tgctgccact ggcccgcatg gtgctggcac
      781 aggccggcga cgaggaccgg aaagaggcat ggcgtcggcg gctgacatcg caaggcgagg
      841 cttacgaggt cgcgctcacg cccttgccgt ttccaggctg gtcgctggcg accgtcattc
      901 ccgaagccga gttcctggga ccggtcgaaa ccacgctgcg cagactgatc atcggccttg
      961 ccgccggcgc gctgttggcg gccctactgt cggccatgct ggtgcgatcg gtgatcgccg
     1021 ctccgctggc ccgcgtggtc ggagaaatcc gccatgtcga gagctttgcc ctggacaagg
     1081 tccgccgtca tccatcgcgg ctggccgaga tatcgagtct ctcgggcgcg atcgccgaaa
     1141 tggcagcggg cctgtccgct ttccgcaaat tcattccggc cgacctcgtc agatcgctgc
     1201 tcagccaggg tgtcgaggca aagcccggcg gatccatcca ggaactgaca gtaatgttcg
     1261 tcgacgtcgc cggctttacc ggactttccg aacgcatggg cgatcgcgtc gtgctgctat
     1321 tatcccgata tctcgacgcc gtctccgaag tcatcgtcgc gaatggcggc acgatcgaca
     1381 aattcatcgg cgatgccgtg atggccttct ggggcgcacc aacggcgcag caggatcatg
     1441 ccgtgcgctg ctgtcgcgcg gcacttgcct gccgcagggc gatcgaggcc gcgggcgtca
     1501 acgacgacca gggtcatccg ctccagatcc ggatcggcat caattccggc cgcatgctgg
     1561 tcggcaatat cggctccgaa ttgcggctga actacaccgt gatcggcgat gccgtgaacg
     1621 tggcgagccg gctcgagagc gccaacaaac agtacggaac gctgatcctg atcggcgcgg
     1681 agaccaggcg cctcatccgg gatgcgttca tcatccgaga aatcgacagc atcgtcgtct
     1741 atggacggac agaggggctt gcggtttacg agctgatcgg ccttgcagaa gtcctcggcg
     1801 aaaacacagc ttggatcgcg agctacgagg aagggctttc gagatatcgt cgtcgcgact
     1861 tctcaggcgc gatcacccat ttcgaggcgg tgcttagcgg gcgccctcac gactggccgg
     1921 cttccctgct gctcgagcgg tgcaagtgtc ttcagcagtc aggcgtggat gaggaatggt
     1981 cgccggtcgc ggcgctaaag gcgaaatgac aggaaagcct gattctggcg ctggacaacg
     2041 cctttctcga ccttcgtgtc gaccgaccgg ctggacgcca tgcgccgcag cgcaatggcg
     2101 cgctccttta gctgggacct ttcagcggcc tgctacagcg cgctgtatcg caagacgatg
     2161 tcgacctgag gtcttcccgt cactctgccg ctttcctgcc cgcgctcatg atatcggtcg
     2221 actgcttctg catcgaagcc atcgacgact tcacgaattc cgtctgcagc ctgacgcatt
     2281 cgactggcga ttccgcctgc gcgagctggc tggcgtgatt gcaaagcgcc gcgacgttag
     2341 cctcggcggc gtcgagtgtc tttctcgcga actcaaacca tccaaccatt gcacgattga
     2401 gttggccgat attgtgatgc gcgagatctc gcggctcgga aagctgcgca ccggattcat
     2461 tagacataca gtttcctctg gcctaaacac tcgtgcgcga acatcgagtc ctttttccta
     2521 tcgtcccgcc ccgacaatgg gcgcggctag ccgcattttc caataccgtt tggctaactt
     2581 ctcgatagga aaagcgtatg gagattcatc aggtccggta tttcctggct gtctgcgagc
     2641 atctgagctt cacccaggcc gcaagacagt gccacgtgac gcagccttcg ctctcacgcg
     2701 cgatccagca tctggagaag gagttcggcg gatatctgtt cgaccggcgc cgctcgaagg
     2761 ttcggctgac cgagctcggc aagctcgtgc agccctacct gcaggaagcc tgggagcagg
     2821 cgcaggccgc aaagaaggag gccaaggact actcgtccaa ggcgccgacg caattgaacc
     2881 tggcgatcat gtgcaccatc gccccccgac tgctgatcca gatgttctcc cgctttcgca
     2941 gcgcgcgccc cgatatcaat ctcgatttga tcgatggcac ggcgcagtcg atcgaggagc
     3001 agctgatcgg ctccaaggcc gaagcggcga tctactgccg gcccgaccgt gccctggatc
     3061 cacgcctgaa ttacctgccg ttgtttcggg aacagatgat gatcgtgctg ccggcagaac
     3121 atcgcttggc tgcgcgcgag accatcgaga tcgccgatct tgccggcgag cgctatgtgc
     3181 agcggtcttt ctgcgagttc aacgacatgg tcgaccgcgt gttcgacgat cgcgggatcg
     3241 attgcgagac ggtctatcgc agtgaccgcg acgattgggt gctcgccatg atcgccagcg
     3301 gcttcggctt cggatttttt ccgagatatt cgatctcaaa tgcagcagtg gtggcgcgcc
     3361 ccctggtcaa tcccgagttc tggcgcgagg tcagcctcac caccgtgaag ggccgcccct
     3421 actcgcgcgc ggtcggcgcg ctggtacatg aagccatgag atcggcgtgg ctcgatgagg
     3481 acgcgctagc ggtcaggaac catccgccgg cttccgaaga cccggaccac gcgtaacaat
     3541 cgtcgacgtc ccgcgtacag acgacatgcg atagcctgtt cgaccttgcg cgcctcgcat
     3601 tgcggtcagc gctggttcac atccacttga tcagacgatt ggcgacggag ggcccttccg
     3661 agcgatcggc cacatccggg ccaaaggaat tgccttatgg aaatgcacca gatccgatat
     3721 ttcctggccg tctgctccga gcgcaatttc acgcgcgccg ccaaactctg ccatatttcc
     3781 cagccgtcgc tgacgcgcgc catcaaactc ctcgaagcgg agttcggcgg cttgctgttc
     3841 cgccgcgagc atccgcattc gcatcttacc gagctcggag aaattgtccg gccccatctg
     3901 cagcaagtct gggaacagag ctatgcggcc atcacgcagg cgcacgaatt caccgccgtc
     3961 ggccgctcaa ggctcaagat cggcatcatg tgcacgatcg cgccagcgct gctgacagat
     4021 ttgctgatgc gcatgcgaat acggcacaag gcgattgaag tggaaatcgt cgacggctca
     4081 gctcgcgacc tggaggaaca gcttgtcggc ggtcacatcg ccgccgctgt gtatggcaag
     4141 ccggacaaga cggactcttg cgtcgctcgg attccgttat ttcgtgaacg gatgatgatc
     4201 gccatgccgc gcgaccaccg gctggctggc cacccatcga tccgcttcgc tgatcttgcc
     4261 ggagtccccc agattcggcg ctcccgatgc gaatacaacg aactggtcgc gcggctcttc
     4321 cggcagcatc ggatcgactg cgaaatcgcg ttcgacagcc cgcgcgacga ttgggcgcag
     4381 gcgttgatcg caggcggcct cggcttcgcg ctgatgccgc aacacagtat cgaccatccc
     4441 ggtgtgctgt cacgaccgct cattgagcca acgctgtggc gggaggcaag cctgtttacg
     4501 tgcagggaac ggacgaacgg cgacggcctt gacgcgctcg tgcaggaagc gacacgaatg
     4561 actaccgcat cggcgagggt tccggccgcc gggaatagcc ttgcggatga tgcacgctag
     4621 tggtggccga tatctcgaga agcgaacgtc gcggtcgatc actcagccgc ctgcggcatc
     4681 aactccatat gctcgtgaaa cacgatgccg gagagcggat cgaattcgcc gacccagggt
     4741 gaacgcgcca gtaccgatct cgtatgcgta tagccggcgt cccagcgttg cttgatgcca
     4801 tccgggctga agtcgatgtc cttggtgtgg gtttcgcgat cgagctgcgg cgcgagcagg
     4861 cgtaccacat gcatccgggt ctgacaccca taacttgcca attcgcgcgc cgcagcgctg
     4921 ctgcgctcgg cctcgggcag gtgcgtgaca agctggttga tgacgtgacg cagccggtgg
     4981 atctgttgct gccgcacgat ctggctggcg atccggctgg aatactgcac atccttttgc
     5041 cggttgaaca cctccgccat cgtggtcggc tccgagcccg ccggattcca caggtggacg
     5101 gagaagatca gcgagtcctt gcgcggatcg tcgtcgaaca cggcctccgt cggcgtgttc
     5161 gataagatgc cgccgtccca gtaaagctcg ccctcgatgc gcacggccgg aaaggccggc
     5221 ggcagcgcgc cggacgccat cacgtgcttc acggtgatcg ttccgtcgcg gctgtcgaaa
     5281 tagcgcatct ggctggtgcg gacatgggcg gcgccgacgg taagccgcgg cttgcagcga
     5341 ttgacgagat caaaatcgac cagctcttcg agcgtctttt gcagcggcgt ggtcgtgtaa
     5401 tagccagcgc gctcgggccc aagcacgtag gcatcgccag catgggcgag cggattgggc
     5461 ctgaagaagc cggcaacccc gtgggtgatc gtcgaccaat aggacagttt ctcgttgaac
     5521 ccgggaaaca cgctaccgaa attccaggcc gggctctgct ccatccgctt ccagaattcc
     5581 tgcaagcgcg agagccgggc tcgaggctca ttgccggcga tcaggctcgc attgatcgcc
     5641 ccgatcgagg tgccgatgat ccagtccggc tcgatgccgg cttcgtgtag cgcctggtag
     5701 acgccggcct gataggagcc gagcgcaccc ccgccctgaa gcaccagcac gacctggccc
     5761 ggtagatcgt tcaccttcgc accacgtccg acaacgtcct gcatcctgct catgtttcgc
     5821 tctccaaagg gccagctatg cgccttcttc gtgacatctt gcgctttgtt acgccgactt
     5881 atgtcaatgt gcggtccagc cgccgtcgac cggcaaggcg acaccggtaa tcgaggctgc
     5941 agcttctgtt gacaggaaca cggtcaacgc accgagctct tctaccgtcg caaagcgctt
     6001 gttgggttgc tgcgcgagga gcacgtcgcg aatcacctgg tcgcgcgaaa taccatgcgc
     6061 cttggcctga ctgtcgatct gcgcttccac caacggcgtg tagacatatc cggggcagat
     6121 cgcgttgcag gtaataccct cttctgccgt ctccagtgcc gtcacctttg ttagaccaac
     6181 tataccgtgc ttggccgcga cataggccgc cttgaagggc gatgcgacaa gtccgtgggc
     6241 ggaggcgata ttgatgatcc gaccaaagcc attctgacgc atcgcaggaa gcgcaaggcg
     6301 cgtggtgtgg aatgccgatg acaggttgat ggccaggatc tggtcccatt tctcagccgg
     6361 aaactggtca agcggcgcga catgctgaat gccggcattg ttaacgagga tatcgagccg
     6421 gccgaactgt gcgacggttg ccgcgatcat ctcggcaatc gacttcggtc gcgtcatgtc
     6481 ggctggtgaa aagctcgcct tgacgccaaa ctcggcggca atctgttccc gcgtcctgcc
     6541 gatctcggcc gcgctgccga gaccgttcag cacgacctcg gcacctactg ccgcgagcgc
     6601 ctgcacaatg ccaaggccga taccgctggt cgagcccgtg accagcgcga ccttgccgtc
     6661 gagcgcccgt cgggcattag acgtctgcgg tttgacagga atattcatgg tccaaaccct
     6721 ccattgatgg cgatcagcct gtgtccgaac tcacgccttt cccaaactct gaaggattgt
     6781 cgaattgtat ggaaatgcct gttggcttcc gaaaccatac gcgcctgcta ttgcgcatgg
     6841 gatgtcattc ccgggcttga tcggctaggc ttggggtcga tcttgccggg gagccaatcc
     6901 atggagatgc accaggtccg ttactttctc gcggtggcgc agcttttgaa tttcacccgc
     6961 gccgccgagg aatgccatgt gacgcagccg tcgctgacgc gcgcgatcaa gcagctcgaa
     7021 gccgagctcg gcggcgacct gttccggcgc gaacggccga cgcaaatgac cgaactcggc
     7081 cagcgcatgc atccgctgct caagcagtgt tatgaggcgg ccatcggcgc gcggtcgctc
     7141 gcttcctcct tcaagagcgg cgagatcggc gcgctctgga tagcgctgac tcattccgtc
     7201 gacctctcgc tgcttattcc ctttctcaat gaggtcaagc ggcagttcaa ccggctggag
     7261 ttcaagtttc tgcgcggcaa aagcggcgag gtcgccgaga tgctgaagaa gggcgaggca
     7321 gagctcggca tcgccgcgga gatcgaccag gattgggacc ggctcgacac ctggccgctc
     7381 ttcaccgagg atttccggct cgtcgtcagc aggcagcatc gtctggcgga gcgcgacagc
     7441 gtcgaactgg aggacctgcg cgccgagcaa ttgttgtcgc gaaattattg cgagcatgcg
     7501 gaacgtctcg gcgcctcgct acgcgagcac ggcctcaacg tcgatcgcgg ccatgaggta
     7561 tccagcgagc gcgacttgat cgagctagtg gaggccgaca tcggtgtggc catcattccc
     7621 gacacctcgc ccattccgca gaccttgaaa cgcacggcgg tcgacggact cgacgcgcgc
     7681 cgcacggtcc atctctatgg agttgctggg cgcgagcgga ccgcggtggc gtctgccatc
     7741 atgcgtatgc tgcgcggcgc cgactggcga ccgagcaaga gggacgttgg catgtagcag
     7801 cggccatttc cctccgaagc gagtttctaa tttgccggct cttcccgggt cagaagtatt
     7861 gatagcgacc tgatcgcggg tgccgcgacc agcacgagcg gcaacatcgt catccatgaa
     7921 atcagccaag acagcagcca gttctggatg aaacctccgg tttctatgaa cggaaaactc
     7981 gctattgcgg ccgcgatcgc gcatgtcaat ccggattgga tgaccccaaa aacaaagtgg
     8041 ctatagcggc gcggaatggc gagcatctga ttgcctccgg agctgcggga aagcaaacgt
     8101 tgtgccatga ctcgcctcct cgatacctaa gcaaaaccga atgtttgagg accgggttgg
     8161 ctggtccgat attggtccgc gggggatgct gttgctttag ggcaccatct tcagagcggc
     8221 gatcaggtcg tcgatcgcca tgcgcttgcg gaagtcggga tcgacgaagc gcgccttcac
     8281 aatcccatcg cggccgacca cgaaggttgc ggggatcggc agcacccagc cgtcattgcc
     8341 gtgaaagctc gccatatcct ggtacgacag cagcttttgg atctcggctc cgagccagat
     8401 cgccagattg agcgacaggg catagccatt gtcgaggtca gtcaggacag gaaatgacgc
     8461 accggagtcc gtcttgaatt tgccggcgaa ggcttgcgtc tccggcatga tcgcaacgac
     8521 ttggccaccc acagccttga ttcggtccat cgcctcgatc acggcgtgga cgttcaaccg
     8581 gcagtagggg caccaatgac cacggaaaaa catcaccgcg accggaccgt ccgccaacag
     8641 cgagggcagg ccgacaagcc gggccgcggc ttggaggatc tcacttccag gttcagctgt
     8701 cgtgcttctt cagggcagcc tggagacctt ggacaaggct gtccccgtcg aacggcttag
     8761 tcaggtaaca aatcgccccg gacctaaccg cttgcgcacg aatccgctca tcggggaagg
     8821 ccgtgaagaa aatgaacggc acgcggtacc cctgggtaag caagtaggct tgcaactcga
     8881 cgctgctcat gccgggcatt tgaccatctg caatcacaca cgagaggtca ttgacgcggt
     8941 tcgaccgcag
//
LOCUS       DASWGM010001058         5226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1108602,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001058 DASWGM010000000
VERSION     DASWGM010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108602"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="VGA15_28455"
     CDS             <1..93
                     /locus_tag="VGA15_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HEX9591662.1"
                     /translation="AKGKKLHDKRESEKKRDWGREKGRLLRARG"
     gene            226..801
                     /locus_tag="VGA15_28460"
     CDS             226..801
                     /locus_tag="VGA15_28460"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX9591663.1"
                     /translation="MNQKNLLEVDWSKIPAPADDGAAAHLKGMTVPPISLIATDDSSV
                     TLSALPGRVVVFAYPRTGEPGKIALVDDWDMIPGARGCTPQTCAFRDLHAELKAAGAR
                     YVFGLSTQSNAYQTEMASRLHLPFSVLSDEKLTLTHALDLPTMDVAGLTLIKRLALIV
                     DDGRITHVFYPVFPPDRNAGDVLAWLKENPV"
     gene            complement(973..1668)
                     /locus_tag="VGA15_28465"
     CDS             complement(973..1668)
                     /locus_tag="VGA15_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX9591664.1"
                     /translation="MTTNSTIENDPARGGPLLAAALLRGEPDRNCPLCPRLLGFREAA
                     RAREPQWFNSPVPSFGDPDARLLIVGLAPGLQGANRTGRPFTGDYAGDLLYATLLEYG
                     FAKGHYRARPDDGLMLVDCRISNAVRCVPPQNKPQPAEINVCRQFLIATIETMPNLRA
                     IVALGRVAHESTLKALGLRAAAAPFAHGAVHQAGVTKLYDSYHCSRYNTNTRVLTPQM
                     FRSVFAKVRAELG"
     gene            complement(1646..2275)
                     /locus_tag="VGA15_28470"
     CDS             complement(1646..2275)
                     /locus_tag="VGA15_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX9591665.1"
                     /translation="MSPASNKIALFIDGANLYATAKTLGFDIDYKRLLKEFQSRGTLL
                     RAFYYTAIIEDQEYSSIRPLIDWLDYNGYTVVTKATKEFIDASGRRKVKGNMDIELAV
                     DAMELAEHVDQIVLFSGDGDFRSLVEAVQRRGVRVTVVSTISSQPPMIADELRRQADV
                     FTDLVELQSKLGRDPSERPPPREPRHHAPQFLQRATTMAPRGDDDEFDD"
     gene            2619..3011
                     /gene="rpoZ"
                     /locus_tag="VGA15_28475"
     CDS             2619..3011
                     /gene="rpoZ"
                     /locus_tag="VGA15_28475"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX9591666.1"
                     /translation="MARVTVEDCIDKVDNRFDLVLLAAHRARMISSGSQLTVDRDNDK
                     NPVVSLREIADSTISPEDLREELVHSLQKFVEVDEPEPDTVPLIGSAGASVDADDTEV
                     AVERMTEEELLKGLEGLAPPEEQPEEDE"
     gene            3314..>5226
                     /locus_tag="VGA15_28480"
     CDS             3314..>5226
                     /locus_tag="VGA15_28480"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX9591667.1"
                     /translation="MAFRRRSPRQMQAATDSVAVAAPSAAPAKPVRSPRSRMMRQYDL
                     VERVRSYNPDTNEDLLNRAYVYAMKAHGTQTRASGDPYFSHPLEVAAILTDLKLDDAT
                     IVAALLHDTIEDTEATRAEIDHIFGHEIGALVEGLTKLKRLELVSREAKQAENLRKLL
                     LAIADDVRVLLIKLADRLHNMRTLEFVPPTARHRIAEETLDIYAPLAGRMGMQEMREE
                     LEDLSFRTLDPEAYAVVMQRLDALAERNRNLIGEIESQLTKNLQNNGMTAHVYGRRKQ
                     PFSIWTKMERKSVGFEQLSDIFGFRVVMNDVEACYRALGVVHTTWPVVPGRFKDYIST
                     PKQNDYRSIHTTVIGPGNQRVELQIRTEEMDQIAELGIAAHAFYKDDVGSPTELLKRE
                     SNAFAWLRHTVGILSESANPEEFLEHTKLELFHDQVFCFTPKGKLIALPRQANVIDFA
                     YAVHTDVGNSAVGCKINGKFAPLSSELQNGDEVEVLTSEAQSAPPSAWESLAVTGKAR
                     AAIRRATRTAVRDQYAGLGRRIVERLFVRAKIEYADDKLKGALPRLARASIDDVMAAV
                     GRGEMKASDVARAMYPDYKEERVARYGAPKKSLAVKLKLKSPPDPARSPSAIPVRGIN
                     SDLPVKFAPNGGAV"
BASE COUNT         1022 a         1590 c         1672 g          942 t
ORIGIN      
        1 gcgaagggca agaagttgca cgacaagcgc gagagcgaga agaagcgcga ctggggcagg
       61 gagaaaggcc gcctgctgcg ggcgcgggga tgatatttcg aatattgtag gatgggttga
      121 acgcagtgaa acccatcacc atggacgttg atgggtatcg cctcatcggg cgcgcattcg
      181 cgccacccgt tggccccacc catcccgcaa ggcaatgagg gcccgatgaa ccaaaagaat
      241 ctgcttgaag tcgactggag caaaattccg gcgccggccg acgacggcgc ggcggcacat
      301 ctgaagggca tgacggtccc gccgatcagc ctgatcgcga ccgacgatag ctcggtgacc
      361 ctgtccgcgc tgccggggcg ggtcgtggtg ttcgcctatc ccagaaccgg cgagcccggc
      421 aagatcgcgc tggtcgatga ctgggacatg atcccgggtg cgcggggctg cacgccgcaa
      481 acctgcgcgt ttcgcgacct ccatgcggaa ttgaaggcgg caggcgcgag atacgtgttc
      541 ggcctgtcga cgcaaagcaa cgcctaccag accgaaatgg cctcgcggct gcatctgccg
      601 ttctcggttt tgtccgacga gaagttaacg ctgacgcatg cgctggattt gccgaccatg
      661 gacgtggcgg gcctgaccct gatcaagcgg ctggcactga tcgtcgatga cgggcgcatc
      721 acccacgtgt tctatccggt gtttccgccg gaccgaaacg ccggcgacgt gctggcgtgg
      781 ttgaaggaaa acccggtttg acaaatgccg ccgtcatccc cgggatgaac gcaattgtgt
      841 tcacccgtga ggtgcgagcc cctcttgggc gagcctcgaa ggatgaagtg tccggcgcat
      901 gtggctatcc ttcgaggcgc gcaagaaaac gagcgcacct caggatgacg gcgtgcgtat
      961 tgcgcgagcc ccttaaccca actccgctct taccttcgca aatacgctgc gaaacatctg
     1021 tggcgtcagc acccgcgtat tggtgttgta gcgcgagcag tgatagctgt cgtaaagttt
     1081 ggtgacgcca gcctgatgca ccgcgccatg cgcaaacggc gccgcagcgg cccgcaagcc
     1141 cagcgccttc agcgtcgatt catgcgcgac ccggccgagc gccacgatcg cgcgcaagtt
     1201 cggcatggtc tcgatggtcg cgatgagaaa ttggcggcag acgttgattt ccgccggttg
     1261 cggcttgttc tgcggcggca cgcagcggac cgcgttgctg atgcggcaat cgaccagcat
     1321 cagcccgtcg tcggggcgcg cccgataatg gcctttggca aagccgtatt cgagcagcgt
     1381 cgcatagagc aggtcgccgg cgtagtcgcc ggtgaagggc cggccggtgc gattggcgcc
     1441 ttgcaggccg ggcgcgaggc cgacgatcag caaccgcgca tcgggatcgc cgaacgacgg
     1501 caccggtgaa ttgaaccatt gcggttcgcg tgctcgcgcc gcctcacgga agcctaagag
     1561 ccttgggcag agcggacaat tgcgatcggg ttcgccgcgg agcagggcgg cggcgagcaa
     1621 cggcccgccg cgcgccggat cattctcaat cgtcgaattc gtcgtcatcg cccctcggcg
     1681 ccatggtggt cgcgcgctgc aggaattgcg gcgcgtgatg acgcggctcc cgcggcggcg
     1741 gccgctcgga tggatcgcgg ccgagcttgg attgcagctc caccagatcg gtgaagacgt
     1801 cggcctggcg ccgcagctcg tcggcgatca tcggcggctg gctcgaaatc gtcgagacca
     1861 cggtaacccg gacgccgcga cgctgcaccg cttcgaccag cgagcggaaa tcgccgtcgc
     1921 cggaaaacag cacgatctgg tcgacgtgct cggcaagttc cattgcatcg actgcgagct
     1981 cgatatccat gttgcccttc accttgcggc gaccgctggc gtcgatgaat tccttggtcg
     2041 ccttggtgac gacggtgtac ccgttgtagt cgagccagtc gatcagcgga cggatcgatg
     2101 aatattcctg atcctcaata atcgcggtgt agtagaacgc gcgcagcagc gtcccgcgac
     2161 tttgaaattc cttgagcagg cgcttgtagt caatgtcgaa gccgagcgtc ttggcggttg
     2221 cgtagaggtt ggcgccatcg ataaagagcg cgattttatt cgaagcaggt gacatggttt
     2281 ctcgcctggt tgttgtttgt tggtcaacgt ttttggcgcg gcggcagcgg gccgagcatt
     2341 tcaaagaact tatacagcca aagcccggcg gatcagtctg aacagacgga gaggtaaggg
     2401 caatagacgt cgtatacttt tgacgtgcaa tgaagcgttt tcttgtccga accgctgttt
     2461 gtgaaggctc aggacactcc ggctggcgag cccccggaca gattagcagc gccgattgcg
     2521 ccggccaaat cataaattgg tcttgcgaag cgcgtctggt gcctataact aacgcccata
     2581 atcaacatat tcggccccat ttaacggagc gacagtcgat ggcgcgcgtc accgtagaag
     2641 actgcattga taaagtagac aaccggttcg acctggtcct gttggcggcg caccgggccc
     2701 gaatgatatc gtccggttca caacttacgg ttgaccgcga taacgacaag aacccggttg
     2761 tatcgttgcg cgaaatcgcc gattcgacca tttcgcccga ggatttgcgg gaagaactgg
     2821 ttcattcatt gcagaagttc gtcgaagtcg acgaacccga gcccgatacc gtgcccttga
     2881 tcgggtcggc cggcgccagc gtcgacgccg acgacaccga ggtcgccgtc gagcggatga
     2941 ccgaggagga actcctgaag ggtctcgaag gcctggcgcc tccggaagag cagcccgaag
     3001 aggacgaata gaccggttgg ctttgtaccg ccggcatccc gcacgacctg atttaccaaa
     3061 aggcccggac atttgtccgg gcctttgctt ttcttgacgt tttcatggtt accatgcgtc
     3121 catctgcggc gcgggcgaat tctgcgacct ctgccttcga tttgatcgga gggcaggcgg
     3181 tttggcgtta gactgcgacc ggacgttcca aagtccggct cccgaccccc gacgggcgct
     3241 ggttggttcg agaacgcgct gggttgtgtt gtgaacaggt cacacctcga ccccgacatg
     3301 aaggcacgat gcgatggcgt ttagacgccg cagtccacgg caaatgcagg ccgcgaccga
     3361 ttcggtcgcc gtggcggcgc cgtcggctgc gccggcgaag ccggtcaggt cgccgcgctc
     3421 gcgcatgatg cggcaatacg atctggtcga gcgcgtcagg tcgtataacc ccgacaccaa
     3481 cgaagacctg ctgaaccgcg cctatgtcta cgccatgaag gcgcatggca cgcaaacccg
     3541 cgcatccggc gacccgtatt tctcgcatcc gctcgaggtc gcggcgatcc ttaccgacct
     3601 caagctcgac gatgccacca tcgtcgcggc gctgctgcac gacaccatcg aggacaccga
     3661 agccacccgc gccgaaatcg accacatctt cgggcacgag atcggcgcgc tggtcgaggg
     3721 cctcaccaag ctgaagcggc tggagctggt gtcgcgcgag gccaagcagg ccgagaacct
     3781 gcgcaaattg ctgctggcga tcgccgacga tgtccgcgtg ctcctgatca agctcgccga
     3841 tcgtttgcac aacatgcgca cgctggagtt cgtgccgccc acggcgcgcc accggatcgc
     3901 cgaggagacg ctcgacatct atgcgccgct tgccggccgc atgggcatgc aggagatgcg
     3961 cgaagagctc gaagacctct cgttccgcac gctcgatccg gaagcttacg cggtggtgat
     4021 gcagcggctg gatgcgctgg ccgagcgcaa ccgcaacctg atcggcgaaa tcgagagcca
     4081 gctcaccaag aatcttcaga acaacggcat gacggcgcat gtctatggcc ggcgcaagca
     4141 gccgttttcg atctggacca agatggagcg caagtctgtc ggcttcgagc aattgtcgga
     4201 tattttcgga ttccgcgtcg tgatgaatga cgtcgaagcc tgctaccgcg cgctcggcgt
     4261 ggtgcacacc acgtggccgg tggtgcccgg ccggttcaag gattacatct cgacaccgaa
     4321 gcagaacgat tatcgctcga ttcacaccac cgtgatcggt cccggcaacc agcgcgtcga
     4381 attgcagatc cgcaccgagg agatggacca gatcgccgaa ttaggcatcg ccgcgcatgc
     4441 cttctacaag gacgacgtcg gctcgccgac cgaactcctg aagcgggaat ccaacgcttt
     4501 cgcgtggctg cgccacaccg tcggcatttt gtcggaaagc gccaatccgg aagagttttt
     4561 ggaacatacc aagctggagc tgtttcacga tcaggtgttc tgctttaccc cgaagggaaa
     4621 gctgatcgcg ctgccgcggc aggctaatgt gatcgacttc gcctacgcgg tgcataccga
     4681 tgtcggtaac agcgcggttg gatgcaagat caacggcaag ttcgcgccgc tgtcgtccga
     4741 gctgcaaaac ggcgacgagg tcgaggtctt gacctcggaa gcccagtcgg cgccgccatc
     4801 ggcctgggaa tcgcttgcgg tcaccggcaa ggcccgggcg gcgatccggc gtgcgacgcg
     4861 cacggccgtg cgcgatcaat atgccggcct tggccgccgg atcgtggagc ggctgttcgt
     4921 ccgcgcaaaa atcgaatacg ccgacgacaa gttgaagggc gcgctgccgc ggctggcgcg
     4981 cgcctcgatc gacgacgtga tggcggcggt cggccgcggc gagatgaagg cgtccgacgt
     5041 cgcgcgcgcg atgtatcccg attacaagga agaacgggtc gcgcgatacg gcgcccccaa
     5101 gaagagcctg gcggtcaaac tcaagctgaa atcgccgccc gaccccgcgc gcagcccgtc
     5161 ggcgataccg gtgcgcggca tcaactccga tttgccggtc aagttcgcgc cgaacggcgg
     5221 cgcggt
//
LOCUS       DASWGM010001059         3888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1371384,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001059 DASWGM010000000
VERSION     DASWGM010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371384"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..617
                     /locus_tag="VGA15_28485"
     CDS             108..617
                     /locus_tag="VGA15_28485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591668.1"
                     /translation="MSITQSAKFLRTVLLIDAATCVATGLLMTLGADIVAGLTAMPAP
                     LLLYAGLSLFAVAIFIALVGTRESLAPPAVWVVIIGNALWVAGSVLLLFGGIIAPNAL
                     GYLFIGAQAAAVAVLAELEYFGLRQTLAVAHWGSTKAPHRESARCFSISPLNSRPTFD
                     HHGQSCSPG"
     gene            780..2564
                     /locus_tag="VGA15_28490"
     CDS             780..2564
                     /locus_tag="VGA15_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEX9591669.1"
                     /translation="MFLRFNRRFKDGKEHRYWNIVESKRCASGKVVQRQVLYLGEIND
                     SQQEAWCRVIEAFDEEAQRHRQLALFPADRRVPEHAQGHGVQVRLDAMELHRPRQWGA
                     CWLACQLYEQLEFDRFWAHRLPNSREGTCWQHILQTLVCYRLIDPGSEWRLHRMWFEQ
                     SAMADLLGADFALVEKNALYRCLDKVLGHKAALFSHLRQRWQDLFGAGFEVLLYDLTS
                     TYFESAPPDDENDKRRHGYSRDKRRDCVQVVIALIVTPDGFPLAYEVLPGNTSDKTTL
                     PGMLRRIEAQYGKAQRIWVMDRGIPTEEQLAAMRQADPPVSYLVGTPKGRLTKLEQAL
                     VALPWQAVRQGVEVKLLPQEQELYVLAQSRARIHKERAMRRRKLKWLWARLKEIAAME
                     LDREELLMKLGAARSKARAAWRLIDINIDPKQAAFSFTLNRDKLRQARRREGRYLLRS
                     NLCGQDPAELWRFYIQLVEVEAAFKNLKDDLKLRPIYHQIEPRIEAHIFVAFLAYCLH
                     VTLRARLKPLAPGLTPRAVLDKLAAVQMLDVHFPTTDGRTLILSRYTELNADHKLLAH
                     QLKLSLPLQPPPRITAPQMPVRLATHSM"
     gene            complement(2709..>3888)
                     /locus_tag="VGA15_28495"
     CDS             complement(2709..>3888)
                     /locus_tag="VGA15_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX9591670.1"
                     /translation="GLTEQQPENNVYRILIEMLAVTLSKKTRARAVQLPAWNEALGLP
                     RPWDQQWSLRMQQILAYETDLLEYGDIFAGSTEIETKVAALKHEAREELQTILRMGGA
                     VAAVENSYMKSRLVESNARRVEAIERGEQIVVGVNRFTETEPSPLAAGEASILTVPPE
                     VETGLVASLQAWRAARDVAAVEAALADLRRAAQAGRNIMEPSIACAKAGVTTGEWGEV
                     LRQGFGEYRAPTGVSHAARNDCRGIDDLRAEVDRVTRKLGRRPKILVGKPGLDGHSNG
                     AEQVAVRARDAGMDVVYDGIRLTPAEIVDAARDRDVHVVGLSILSGSHMPLVEDVMTR
                     LRAAGLDEVPVVVGGIIPPDDAVALRQAGVAAVYTPKDFELNRMMSDVLRIVERSNAR
                     "
BASE COUNT          701 a         1256 c         1196 g          735 t
ORIGIN      
        1 cgcgcgctcc aattacctct gacgtaatcg acggacggca cggctccgcg caatgttcgc
       61 tgccaagagg tcggcagacc tcgatcacca aacgaaagga gcattccatg tcgatcactc
      121 aatccgcgaa gtttctgcgc accgtgctgc tcatcgacgc cgcaacatgc gttgcaaccg
      181 gcctcttgat gacgctcggc gccgatatcg tggcggggct cacggcaatg ccggcgccgt
      241 tgctgctcta tgcgggcctc agccttttcg ctgttgcgat cttcatagcc ctggtgggta
      301 cgcgcgagag ccttgcgcct ccggcggttt gggtcgtcat catcggcaac gccttgtggg
      361 tggcagggag cgtgctcctg ctgttcggcg gcatcattgc gcctaatgct ttgggctatc
      421 tcttcatcgg cgcccaggcc gccgcggtcg ccgtattggc cgaactcgaa tatttcggcc
      481 tgcggcagac cttggcagtt gcccattggg gttcaacgaa agcaccacac cgcgagtcgg
      541 cgcggtgctt ttcgatcagc ccgctcaatt cccgaccgac cttcgatcac cacgggcaga
      601 gctgtagccc gggttgagcg ctagcccaac tttcccaact cgcgtagcga aacggcaatt
      661 tttggggtgt gaaggggcgc aaacgcgagt caaatcaacg aggcattctg gcaaatcgtc
      721 tgtagtgtag accaagtatc tttttcgtca tttcgggttg gcgtaagttg attcgcatca
      781 tgttcttgcg gttcaaccga cgcttcaaag acggcaagga acatcgctac tggaatattg
      841 tcgagagcaa acgttgtgcg tcgggcaagg tggtgcagcg tcaggtgctg tatctgggcg
      901 agatcaacga cagccagcag gaagcgtggt gccgggttat cgaagcgttt gacgaagagg
      961 ctcagcgcca cagacagctc gccctgtttc ccgccgatcg cagggtgccg gagcatgcac
     1021 agggccatgg cgtgcaggtg cggctcgacg cgatggaatt gcatcggccg cggcaatggg
     1081 gcgcgtgctg gttggcgtgt caactttacg agcagctcga atttgatcgg ttctgggcgc
     1141 atcgattgcc gaactcgcgc gaaggcacgt gttggcaaca catccttcag acgctggttt
     1201 gctatcggct gattgatccc ggcagcgaat ggcgcttgca ccggatgtgg ttcgagcaga
     1261 gtgcgatggc ggatttgctg ggcgctgact ttgcactggt cgagaagaat gcgctctacc
     1321 gctgcctcga caaggtgttg gggcacaagg cggcgctgtt ctcgcatttg cgccaacgct
     1381 ggcaggacct gttcggggcc ggcttcgaag tgctgctgta cgacctcacc agcacctatt
     1441 tcgagtcggc gccgccggac gatgagaatg acaagcgccg ccacggctac agccgcgaca
     1501 agcgccgcga ttgcgtgcag gtggtgatcg cgctgatcgt gacgccggac ggttttccgc
     1561 tcgcctacga ggtgctgccc ggcaacacct ccgacaagac gacgttgccc ggcatgctgc
     1621 gccggatcga agctcaatac ggcaaggcgc aacgcatctg ggtgatggat cgcggcattc
     1681 cgaccgaaga gcaactcgcc gcgatgcggc aggccgatcc gccggtctcc tatctggtcg
     1741 gcaccccgaa gggacggctc accaaactgg agcaggcgct cgtcgccctg ccgtggcaag
     1801 cggtgcgcca gggtgtggag gtgaagctct tgccgcagga gcaggagctt tatgtgctgg
     1861 cgcagagccg cgcccgcatc cacaaggaac gcgccatgcg gcggcgcaag ctcaaatggc
     1921 tatgggcacg cctcaaagag atcgcggcga tggagcttga ccgcgaggaa ctgttgatga
     1981 agctcggcgc cgcccgcagc aaggcaagag cggcatggcg gttgatcgat attaacatcg
     2041 atcccaagca ggccgccttc agtttcacgc tcaatcgaga caagctgcgc caggcgcgcc
     2101 ggcgcgaagg tcgctatctg ctgcgcagca atttgtgcgg acaggacccc gctgagctgt
     2161 ggcggttcta tatccaactt gtcgaggtcg aggcggcgtt caagaacctc aaggacgatc
     2221 tcaagctgcg cccgatctat caccagatcg agccgcgtat cgaagcccac atcttcgtcg
     2281 ccttcctggc ctattgcctc cacgtcacct tgcgggcgcg gctcaagccg ctcgcccccg
     2341 gcctcacccc gcgcgctgtg ctcgacaagc tcgccgccgt gcagatgctc gacgtacact
     2401 tcccaaccac agatggccgc accctgattc tcagccgtta taccgagctg aacgcagatc
     2461 acaagctcct cgcccaccaa ctcaagctca gcctgccact ccaaccgccc cctcgcatca
     2521 ccgcaccaca aatgccggtt cgcctcgcaa cccattcaat gtagtgtaga ccttcgggga
     2581 gcctccgctg attcttctca gctttttcct ccagttggga aagttgggct agcgaaaccc
     2641 ggggacggca agtgccatat ggatcaggca gtcccggatt tcgctggcgc tcaatccagg
     2701 ctacggattc accgcgcatt actgcgttcc acaatccgca gcacatcgct catcatgcgg
     2761 ttgagctcga aatccttcgg ggtatagacg gcggcgaccc cggcctgcct gagcgcgacg
     2821 gcgtcgtcgg gcggaatgat gccgcccacg acgaccggca cctcatcaag gccggcagcg
     2881 cgcaggcggg tcatcacgtc ttcgaccagc ggcatatgcg agcccgacag gatcgacagt
     2941 ccgaccacgt gcacatccct gtcgcgggcg gcgtcgacga tttccgcagg cgtcaggcgg
     3001 atgccgtcat agaccacgtc catcccggcg tcgcgcgccc gcactgccac ctgctcggcg
     3061 ccgttggaat ggccgtcgag ccccggtttg ccgaccagga ttttcggccg tcgtccgagc
     3121 ttgcgcgtca cccggtcaac ctcggcgcgc aaatcgtcga taccccggca gtcgttgcgc
     3181 gccgcgtgcg aaacgccggt cggggcgcgg tattcgccaa aaccctgacg caacacttcg
     3241 ccccattcac cggtggtgac gccggccttg gcgcaggcga tcgacggctc catgatgttg
     3301 cggccggcct gcgcggcgcg ccgcagatcg gcgagtgcag cctccaccgc cgcaacgtcc
     3361 cgcgcagcgc gccacgcctg caggctcgcg accaggccgg tctcgacctc gggcggcacc
     3421 gtgagaatgg aggcctcacc cgccgcgagc ggcgaaggct cggtttcggt gaagcggttg
     3481 acgccgacca cgatctgctc accgcgctcg atcgcctcca cgcggcgcgc attgctctcg
     3541 acaagccgcg atttcatgta gctgttctcg accgctgcca ccgcaccgcc cattcgcaga
     3601 atggtctgga gttcctcgcg ggcttcgtgc ttgagcgccg ccaccttggt ctcgatctcg
     3661 gtcgagccgg cgaagatgtc gccgtattcc aacaagtcgg tctcgtaggc gaggatctgc
     3721 tgcatacgca gcgaccattg ctgatcccac ggccgcggca ggccgagcgc ctcgttccag
     3781 gcagggagtt gaacggcgcg ggcgcgagtc ttcttcgaca gcgtcaccgc cagcatctcg
     3841 atgaggatgc gatagacgtt gttctccggc tgctgctcgg tgaggcca
//
LOCUS       DASWGM010001060         4718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2759205,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001060 DASWGM010000000
VERSION     DASWGM010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759205"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VGA15_28500"
     CDS             <1..625
                     /locus_tag="VGA15_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX9591671.1"
                     /translation="AEQWHVAELKEELKRVLDIDLPVDAWAKEEGIADEELLSRIEQR
                     VDEHMAAKVAQWGPDVMRYVEKTILLQTLDHLWREHLVMLDHLRQVIGLRGYGQRDPL
                     QEYKSEAFSLFEAMIAHLREAVTAQLMRVEIVPPEEQPALPHMEAHKLDPNTGEDELA
                     FANASLVPTATADRDPKNPASWGKIGRNEDCPCGSGKKYKHCHGKYA"
     gene            complement(805..2283)
                     /locus_tag="VGA15_28505"
     CDS             complement(805..2283)
                     /locus_tag="VGA15_28505"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HEX9591672.1"
                     /translation="MTNRSLVRALITSVALTAGVLLSGCNSDEISLANNAKANQPVPP
                     KLVADMAEKNMDLQSPMLVRLFKQEAELEVWKQDRAGRFALLKTYPICRWSGDLGPKV
                     REGDRQAPEGFYSISPGQMNPQSAYYLSFNTGYPNAFDKALGRSGSQLMVHGDCSSRG
                     CYAMTDEQIAEIYSLGRESFFGGQRAFQLQAYPFKMTALNMARHRNNPNMPFWKMIKE
                     GYDHFEVTRQEPKVEFCEKKYVFDAAKPPDAAKDPVFEASAKCPAYVIPDEVASAVAE
                     KQQADQAETAKLIAQGTPMARINTGIDGGMNKVFASKLPDGSTGLSEGGDGQGLSLVA
                     LSRAPGTIPSHVNPPRGPVTSPDEPLAASLAPAPAPATRVASAAPNAQSDNFFSSLAR
                     KVGLGAADTAAASPQPAVPAKPKIIEAKRSDQVASVPKTAAAATPKPDTTKQAAARPP
                     LKPALADPPPPPAAAPAKDSLVAGSQPIVSPNSFDSRFQSFR"
     gene            complement(2551..3513)
                     /locus_tag="VGA15_28510"
     CDS             complement(2551..3513)
                     /locus_tag="VGA15_28510"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX9591673.1"
                     /translation="MPDHMRSYLDFEKPVAELDSKVDELRALAATGSDIGEEISRIED
                     KAAQALSDLYANLTPWQKTLVARHPQRPHLTDFIAALITEFTPLAGDRKFGEDEALVG
                     GFGRFRGESICVIGQEKGATTESRIKHNFGMARPEGYRKAVRLMELADRFGLPVLSIV
                     DSAGAYPGIGAEERGQAEAIARSTDACLSLGVPNVAIITGEGMSGGAIAITTANRVLM
                     FEHAIYSVISPEAASSILWRDGTKAQEAATSMKITAQDMLRFGVIDSILREPSGGAHR
                     DPHTMIAATGDAIAQALNDLRNLDPDTIRKQRRQKFLDIGRKLG"
     gene            complement(3715..4674)
                     /gene="xerD"
                     /locus_tag="VGA15_28515"
     CDS             complement(3715..4674)
                     /gene="xerD"
                     /locus_tag="VGA15_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX9591674.1"
                     /translation="MRSSKTSDARWINLFLDMLAAEQGAGHNTLDAYRRDLTDFSEFL
                     ARAGRGFAGAETQALRDYLDDLDARGFKSSSVARRLSAMRHLFRFLLNERVRSDDPAA
                     ILSGPKRGRGLPKVLSIADVDRLLVRAKALADAPEASAPQRLRAKRLYCLLEVLYATG
                     LRVTELVTLPLSASRRDARMIVVRGKGNKERLVPLNEASRQAMADYLAAIEALKPEKK
                     KNAAASKWLFPSFGESGHLTRQHFARDLKELAAAAGLAPRLVSPHVLRHAFASHLLHN
                     GADLRIVQTLLGHTDISTTQIYTHVVEERLKSLVRDLHPLAEK"
BASE COUNT          844 a         1420 c         1562 g          892 t
ORIGIN      
        1 tgccgagcag tggcacgtcg ccgagctgaa ggaagagttg aagcgggtgc tcgacatcga
       61 tctgccggtc gacgcgtggg ccaaggaaga aggcatcgcc gacgaggaac tgctgtcgcg
      121 catcgagcag cgcgtcgatg agcacatggc ggccaaggtg gcgcagtggg gccccgacgt
      181 gatgcgttac gtcgagaaga ccatcctgct gcagacgctg gatcatctgt ggcgcgagca
      241 tctggtgatg ctcgatcatc tgcgtcaggt gatcggcttg cgtggctatg gccagcgcga
      301 tccgctgcag gagtacaagt cggaagcgtt cagcctgttc gaggcgatga tcgcgcatct
      361 gcgcgaggcg gtgacggcgc aactgatgcg cgtcgaaatc gtgccgccgg aagagcagcc
      421 ggcactccct cacatggaag cccacaagct cgatccgaac accggcgagg acgaactggc
      481 cttcgccaat gcctcgctgg tgcccacggc caccgccgac cgcgacccga agaatccggc
      541 gagctggggc aagatcggcc gcaatgagga ttgtccctgc ggctcgggca agaagtacaa
      601 gcactgccac ggcaagtatg cgtgaggcgc gcttggatta cgcttgatcg tgcgccgacg
      661 tcaccatccg cgaaagaagc gtaggtacag ggtgggcaaa ggcgctcttc gcgccgtccc
      721 accaaaatat tcaacagcga tgatggtctg tgacagtggg ctcgcttcgc ctagcccacc
      781 cactgattct gcgcgagttg cggcttacct aaacgactgg aaccggctgt cgaacgaatt
      841 cggcgacacg atcggctgtg agccggcgac caggctgtct ttggcggggg ccgctgcggg
      901 tggtggtggt gggtcggcga gcgccggctt cagtggtggc cgggcggcgg cctgcttcgt
      961 ggtgtcaggc ttcggcgttg cggcggcggc ggtctttggc accgaggcaa cctggtcgct
     1021 gcgcttggcc tcgatgattt tcggttttgc tggaaccgcg ggctgcgggc ttgccgctgc
     1081 ggtgtcggca gcgccgaggc cgaccttgcg ggcaaggctg ctaaagaaat tgtcggattg
     1141 cgcattcggc gccgcggatg cgacgcgcgt cgcgggtgcg ggggccggcg ccagcgaggc
     1201 cgccagcggc tcgtccggcg acgtaaccgg cccgcgcggc ggattgacat gggagggaat
     1261 ggtgccgggc gcgcgggaaa gcgcgaccag cgacaggccc tgtccgtcgc cgccttccga
     1321 cagtccggta ctgccatcgg gcagcttgga ggcgaacacc ttgttcatgc cgccgtcgat
     1381 gccggtgttg attcgggcca tcggggttcc ctgggcaatc agcttggcgg tctcggcctg
     1441 atccgcctgc tgcttctccg ccacggcgct ggcgacttca tccgggatca cataggccgg
     1501 gcatttggcg gaggcttcga ataccggatc cttggcggcg tccggcggct tcgcggcgtc
     1561 gaatacatat ttcttttcgc agaactcgac cttcggctcc tgccgcgtca cctcgaaatg
     1621 atcgtagcct tccttgatca ttttccagaa cggcatgttc ggattgttgc gatgcctggc
     1681 catgttcagc gccgtcatct tgaacggata ggcctgcagc tggaaggcgc gctggccgcc
     1741 gaagaaggat tcgcgaccca acgaatagat ttccgcgatc tgttcgtcgg tcatggcgta
     1801 gcagccgcgc gacgagcagt cgccatgcac catcagttgc gagcctgagc gtcccagcgc
     1861 cttatcgaag gcgttggggt agccggtgtt gaacgaaaga taatacgccg actgcggatt
     1921 catctgtccc ggcgagatcg aatagaagcc ttccggcgcc tggcgatcgc cttcgcggac
     1981 cttgggtccg aggtcacccg accagcggca gatcgggtag gttttcagaa gcgcgaagcg
     2041 gccggcgcga tcctgcttcc agacctcgag ttcggcttcc tgcttgaaca gccggaccag
     2101 catcggcgat tgcaggtcca tgtttttttc cgccatgtcg gcgacgagct ttggcggcac
     2161 cggctggttg gccttggcgt tattcgccag cgagatttcg tcgctgttgc agccggacag
     2221 cagcacacca gccgtgagcg cgacagatgt gatgagcgcg cgaaccagcg agcgatttgt
     2281 caatgcagag ctccacaccc tacgggcaat ttgcgcaccc caatcatagc gttcatgccc
     2341 tgaagccatt gattaaaata ctatcctttg gggcccttgc tcgcaagccg gtcgagccgt
     2401 cacggcagtt acgcatcagg catggtcaac agacgttaaa tatcggtgtc tgggcctaat
     2461 tggggccgag gctgtcgatt ttcggaaaag ccgggtttat acgggccaaa cggccgtttt
     2521 ggcggccatt tcccgcagaa aagccgcgtg tcagcccagt ttgcggccga tatcgaggaa
     2581 tttctgacgc cgctgcttgc ggatcgtgtc cggatccaga ttgcgcaaat cgttcagggc
     2641 ttgcgcgatg gcatcgccgg tggctgcgat catggtgtgg ggatcgcggt gcgcgccgcc
     2701 cgaaggctcc ctcagaatcg aatcgatcac gccgaatcgc agcatatcct gtgcggtgat
     2761 cttcatgctg gttgcggctt cctgggcctt ggtcccgtcg cgccaaagga tcgaggacgc
     2821 tgcctccggc gaaatcacgc tgtagatggc gtgctcgaac atcagcacgc ggttagccgt
     2881 ggtgatggcg atggcgccgc ccgacatgcc ttcgccggtg atgatcgcga cattgggtac
     2941 gccgagcgac agacacgcgt cggtagagcg cgcaatcgct tcggcctgtc cgcgttcctc
     3001 cgcgccgatg cccggatagg cgccggcgga atcgacgatc gacagcacgg gaagcccgaa
     3061 tcgatcggcc aattccatca gccggaccgc cttgcggtag ccctcgggac gcgccatgcc
     3121 gaaattatgc ttgatgcggg attcggtggt ggcgcccttt tcctggccga tcacgcagat
     3181 gctctcgccg cggaagcggc caaatccgcc caccagtgct tcgtcctcgc cgaacttgcg
     3241 gtcgccggcc agcggtgtga attcggtgat cagcgcggca atgaaatcag tcaaatgggg
     3301 gcgctgcgga tggcgcgcca ccagggtctt ctgccacggc gtcagattgg catagagatc
     3361 gctcagcgcc tgggcggcct tgtcctcgat ccgcgaaatc tcttccccga tgtcgctgcc
     3421 ggtcgccgcc agtgcgcgca gttcatcgac cttggaatcg agttcggcga cgggcttttc
     3481 gaaatcgaga taactgcgca tgtgatccgg catcaaatca atataaggac gaacgcggtt
     3541 tcaggcgaag cggatttcgg ttcgtgtcgg gaaagcacgc atcttcaatg gtttagcgcg
     3601 cattccgacg cgagggcgag gccgctttgc gaaagtgcga gcaaagcagg tcgcgggcgg
     3661 gaaatggctc tttcacggca gaagggaaag aagtcaaggc ggcaaagcgt aaggctactt
     3721 ctccgccagc ggatgcaggt cgcggaccag gcttttcagg cgctcctcga ccacatgggt
     3781 gtagatctgc gtggtcgaga tatcggtatg accaagcagc gtctgcacga tgcgcaaatc
     3841 ggcgccgttg tgtaagaggt ggctggcgaa ggcatgacgc aacacatgcg gtgagaccag
     3901 ccgcggcgcc agtccggcgg cggcggcgag ttccttgagg tcgcgcgcga aatgctgccg
     3961 cgtcaaatgt ccgctctccc cgaacgaggg aaacagccac ttcgaggcgg cggcgttttt
     4021 cttcttctca ggcttgagcg cttcgatcgc ggcgaggtaa tcggccatcg cctgtcgcga
     4081 tgcctcgttc agcggtacca gccgttcctt gttgcccttg ccgcgcacca cgatcatgcg
     4141 ggcgtcgcgt cgcgacgccg agagcggcag cgtcaccagt tccgtcaccc tgaggccggt
     4201 ggcgtagagc acctcgagca ggcaatacaa tcgcttcgcg cgaagccgct gcggcgcaga
     4261 ggcttccggc gcatcggcca gcgccttggc gcgcaccagc aggcgatcga cgtcggcgat
     4321 cgacagcacc ttcgggaggc cgcggccgcg cttcggcccc gaaaggatcg cggccggatc
     4381 gtcgctgcga acccgctcgt tcagcaggaa acgaaacagg tgccgcatcg ccgacagccg
     4441 gcgtgccacg ctcgacgatt tgaagccgcg cgcgtcaaga tcgtcgaggt agtcacgcag
     4501 cgcctgggtt tcggcgccgg caaagcccct gccggcacgg gcgagaaatt ccgaaaaatc
     4561 ggtgaggtcg cggcgatagg catcgagcgt gttgtggccg gcgccctgtt cagccgccag
     4621 catgtcgagg aacagattga tccatctggc atctgaggtc ttgctggaac gcatcccctg
     4681 gtgtccccca tcggaagttc gcctctcacg ggcgatcg
//
LOCUS       DASWGM010001061        16301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_554153,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001061 DASWGM010000000
VERSION     DASWGM010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554153"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VGA15_28520"
     CDS             <1..933
                     /locus_tag="VGA15_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX9591675.1"
                     /translation="TGVKVFNWLFTMYGGRVVYSASILWSIGFMVTFVIGGMTGVLLA
                     VPPADFVLHNSLFLVAHFHNVIIGGVLFGAFAGYTYWFPKAFGFRLHEGLGKAAFWCW
                     ITGFYVAFMPLYVLGLMGMTRRMQHYDVPAWRPWLLIAAGGAVIILAGIILQIAQLAV
                     SIRRREALRDQTGDPWDGRSLEWATPSPPPSFNFAVLPRIQGEEAYWAIKQRARQQAK
                     LREESGYTEIEMPRNSPTGFICAFFATLMGFALIWHIWWIVALAALGAYATFVVFAWR
                     DRHEYIIPADVVARVDRANRSARSEALAQLWTAP"
     gene            930..1601
                     /gene="cyoC"
                     /locus_tag="VGA15_28525"
     CDS             930..1601
                     /gene="cyoC"
                     /locus_tag="VGA15_28525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEX9591676.1"
                     /translation="MSGLEVTAGRGDPNRAGYHRELRDRRHPAGHAAGALVSKRIIVG
                     YGFWIFLLSDIIMFSAFFATYAVLVGQTAGGPSGRELFDLRNTGIETACLLLSSFACG
                     LASIGAQTHRGSWFYGAMAATFILGAAFIGIEVREFAGLVVRGAGPTRSAFLSAFFTL
                     VGCHGLHVTAGLLWLLTMVAQVFAKGYRADILRRVLCFSLFWHALDIVWVALFTMVYL
                     MGAAA"
     gene            1598..1999
                     /gene="cyoD"
                     /locus_tag="VGA15_28530"
     CDS             1598..1999
                     /gene="cyoD"
                     /locus_tag="VGA15_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEX9591677.1"
                     /translation="MSHSEQKRDSNHADTAPGDERLDGHEAAGGIGTYLIGLGLATLL
                     TIVSFFVARTTLVWQPSIPVALAVLAIAQMGVHLVFFLHITTGPDNVNNVMALAFGVL
                     IVLLVITGSLWIMAHMNHNMMPMDQIMQMQR"
     gene            complement(2166..3650)
                     /locus_tag="VGA15_28535"
     CDS             complement(2166..3650)
                     /locus_tag="VGA15_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX9591678.1"
                     /translation="MTLKYSLFGSALAGVMLLAVDPSVAQTNEQLQQQIRELQRQLQN
                     LQNQVEEQSRRAQAPAAAPVAPPPSGVKAVLTPANRPGICSADDANCIYLTGRLHFDV
                     ADYLSVHPQTPAGLHSLTSGVNARRARIGVLGKFMADWNYAFVADFGGSADGPQLSNI
                     ETASLSYQGLRPFAFEIGYMDVPWALDDATSTNDIMFLERASPEVVATGLAAGDFRSA
                     AGVRWNTDRAWAGAYATGPLSGATHTGSNQQQLGGLARATYQALQGGNYSLHLGVDGE
                     YVFVPRANGSSATSIASTMTLSDRPELRVDPTIFLNTGPIPAKNAGAYGVEAAGGFGS
                     FYAQGEYYRYFVNQAGLAAGAPTPQLNFDGGYIEASYTVTGESRKYIPTTGAYSAIVP
                     DRPLSFTAGGWGALELAVRYSYVNLNNHATPGVAPTTTGGVFGGRQTIYTLGVNWYPI
                     TNIRFMLNYIYATVNKIPTATAVGGSTLPGAVIQAIAMRTQVAF"
     gene            complement(4374..4985)
                     /locus_tag="VGA15_28540"
     CDS             complement(4374..4985)
                     /locus_tag="VGA15_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX9591679.1"
                     /translation="MDLYSHRHFTIDDIDAVDIARAQEYALLATVLGRAPDADLLRRL
                     AELCGDPSPLGVAHAALAEAARCATREGVEREHFDLFIGLGRGELLPYGSYYLTGFLH
                     ERPLARLRYDLARLGIERAPGRAEPEDHAAILCEIMAGLVGGRLLAPRGADRALFEAY
                     LAPWMEKFFADLELAKTAEFYRSIGALGRVFMAIEAKAFAMSS"
     gene            complement(5050..5715)
                     /locus_tag="VGA15_28545"
     CDS             complement(5050..5715)
                     /locus_tag="VGA15_28545"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEX9591680.1"
                     /translation="MSRREDFLARWSRRKRETAASANGPKQSDCKARAQSGDAPISRK
                     TTADPTGPAFDLAKLPSIESITAETDIRNFLAPFVPPELTRAALRRVWTTDLKIRDFV
                     GLADYDWDFNAPGSMAGFGSLEPTDALRRQAADLIGRGAAEGNPRVSKITEAKPISSG
                     SVPDRPDPRKPINDSLTSPQNNADSRGATQLRGATNATIDQREKPELPLIGRRRHGQA
                     LPK"
     gene            complement(5721..6230)
                     /locus_tag="VGA15_28550"
     CDS             complement(5721..6230)
                     /locus_tag="VGA15_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEX9591681.1"
                     /translation="MSVVPALARIPVGVVVERRRATSPWIDFTWQPVMALPGRPDAAP
                     WTVLSIDAGIATFYVGSTEIALYRTEGRNYRDNLACDVPLLWIALRPTGLEPPYDLLA
                     VTADPAEGESFTQTGRDLVATVSMPAAVRDSVEAFVAAHLVEQPFHKRKRNPADPEAL
                     ARRRPKARD"
     gene            complement(6316..8304)
                     /locus_tag="VGA15_28555"
     CDS             complement(6316..8304)
                     /locus_tag="VGA15_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX9591682.1"
                     /translation="MPLDAEAVRRGCRGSQVTTARQLCRTELERFRAAAAGGGPLIVA
                     CTQEAPLFSEVAEGHESGADIRYANIRETAGWSAEAAKASAKMAALIAAAAEAAPEIS
                     VVKFNSEGVTLVYGRDEQAIEAANLLKQHLDVTVLIKPPAALQPPAVTDFPVAKGEIR
                     SAKGHLGAFELTVDKFGQASPSSRGVLAFGPTKDGATSRCDIVLDLSGGAPLFPAADL
                     RDGYLRADPGSPAAVVKAVLRARDLVGSFEKPRYITFADDLCAHSRSQIVGCNRCLNL
                     CPTGAIRPAGDHVAIDAQICAGCGQCASVCPTGAASYALPPADALMRRLRALLTAYRD
                     AGGKRAIVLLHDEAHGTPLIDALARFGDGLPANVLPLAVNEVTQVGLEVIAAAFAYGA
                     SALRLLLRAKPRHDVASLATTLALAEPILVGFGFGPGRAATVDTDDPDMLGEMLRAIA
                     APDPAPRPATFLPIGGKRDVLRMALRELRAAAPASAEVIALPEGAPFGAVEVNVEGCT
                     LCLACVSACPTGALLDDPERPMLRFMEDACVQCGLCKATCPEKVITLKPQLDILATLG
                     PARVLKQEEPFHCIRCGTPFGTKSSIERVAAKLAGKHWMYQGKDRRIDALKMCGECRV
                     IAVAEAEFDRSGAPARPKIRTTEDYLREREQQTQKGET"
     gene            complement(8388..9479)
                     /locus_tag="VGA15_28560"
     CDS             complement(8388..9479)
                     /locus_tag="VGA15_28560"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX9591683.1"
                     /translation="MSEKTVKGQGMATHCMRTPLAMCTLICAFIFLGIALIAAPVNAQ
                     QPQSQPQTPSAAQAPLVDPDASAVKEQNLLRESPRIEGTIVIPEPRERVLIQPAGRGW
                     RFFHEVLLHWIGTVVIVGAIALLAIAYLSIGRIRISAGRSGRKVRRFNAFERFSHWLT
                     AASFVVLALTGLNITFGKLLLLPLLGPDAFSRVSEAAKYVHNFVSFPFVAGLVLIVAL
                     WIKDNIPRKIDIVWLKEGGGFVGSKRPQAGRFNAGEKLVFWFAAGGGAAVALSGYLLL
                     FPFYVTNIAGMQLAQIVHSLIAVFFIAVILGHIYIGTLGMEGAFEAMGTGSVDLNWAE
                     EHHRIWLEEEIAKGRGPTPAPEASTSPAE"
     gene            complement(9514..10110)
                     /gene="fdh3B"
                     /locus_tag="VGA15_28565"
     CDS             complement(9514..10110)
                     /gene="fdh3B"
                     /locus_tag="VGA15_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HEX9591684.1"
                     /translation="MARMKFLCDADRCIECNACATACKNENEVPWGLNRRRVVTINDG
                     KPGERSVSMACMHCTDAPCQAVCPVECIYTTADGVVLHSKDQCIGCGYCFYACPFGAP
                     QYPQVSNFGSRGKMDKCTFCAGGPEVHNSPEEYAKYGANRLAEGKLPLCAEMCSTKSL
                     LAGDGETVAGIYKERVETRGYGSGAWGWTTAYGADTGI"
     gene            complement(10122..13019)
                     /locus_tag="VGA15_28570"
     CDS             complement(10122..13019)
                     /locus_tag="VGA15_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX9591685.1"
                     /translation="MLIKRAERAARRGTLVGALAAESGGIDRRAFLRRSGLAAGALAA
                     IGTLPVSGVRNAEAGPPPKSGIPVETRKSICTHCSVGCTVTAEVQNGVWLGQEPSWDS
                     PINRGSHCSKGASVRELVSGERRLKYPTKLVDGKWVRISWDQAIGEIGDRLMAIRQTS
                     GPESVYWLGSAKFSNECAYLFRKFAAFWGTNNADHQARICHSTTVAGVANTWGYGAMT
                     NSFNDIRNSKTLLIMGGNPAEAHPVSLQHLLEGKELNRANFIVIDPRMTRTAAHATDY
                     VRIRPGTDIPVLWGMLWHIFQNGWEDKEFIRQRVYGIDEIRREVAKWPPEEVERVSGV
                     PGEQLKRIAEKFAKEKPATLIWCMGQTQHTVGTANVRASCIALLVTGNVGAFGNGANI
                     FRGHDNVQGATDVGLDVTTLPFYYGLDEGAWRHWCRVWDIDYDWVQSRFISKKMMETA
                     GIPSTRWFDATLLAKDQIDQPDNLRAMMVFGHAGNTITRMPEAAKGIEALDLLVVVDT
                     VPTTWAVLSERKDGVYLLPSGTQFESSGTRTASNRSLQWYDRVVAPIFEAKEDYETIY
                     LLARKLGFADQLFKNIKVVNNLPSAEDLLREINRGGWSTGYTGQSPERLKLHRDHQKD
                     FDLVTLRASAGPCKGEYYGLPWPCWGTPELKHPGTPTLYNTSLHVKDGGGTFRARFGV
                     ERNGVTLLAEGSYSAGSEIRDGYPEFTMAVLKKLGWDKDLTPQELAVIAKVGGNNTDA
                     VAWSTDLSGGIQRVAIEHGCVPHGNGKARAVAWNLPDPVPVHREPIYSPRVDLVGEYP
                     TLPDAKQFRVPNIGFTVQKSAVDRGVAKQFPLILSSGRLVDHEGGGEETRSNRWLAEL
                     RQDMYVEISPADAIERGIKDGAWVWVTGAEYASKARMKALVTERVGKGVAWMPYHFAG
                     WFQGVDQRRKYPQGADPFVLGESANTVTTYGYDPVTNMQEPKVTLCQITLA"
     gene            complement(13019..13237)
                     /locus_tag="VGA15_28575"
     CDS             complement(13019..13237)
                     /locus_tag="VGA15_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX9591686.1"
                     /translation="MDADDKIVFRRRSFLRVLAAGGWAAAASTAPLGGHAVADTLSYD
                     ERRKPLYRETEEVKTFYRVNRYPTERRR"
     gene            13290..13619
                     /locus_tag="VGA15_28580"
     CDS             13290..13619
                     /locus_tag="VGA15_28580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591687.1"
                     /translation="MAEHALSTGRLFLSHHLDRPLFAQIGNRSVLNKQNSYDRTMPKD
                     PPIELTDIATIGSAFASLSVNKVFGWMRLLSRLKPWEAASVRTYRFGGKNDERTLLNP
                     QEVVANG"
     gene            complement(14582..15862)
                     /locus_tag="VGA15_28585"
     CDS             complement(14582..15862)
                     /locus_tag="VGA15_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX9591688.1"
                     /translation="MTATDRLILAGRDSLAGRGGGMRSILPFAGPAIVASVAYMDPGN
                     FATNIEAGAKYSYNLLWVVVFANAVAMLFQALSAKLGIVTGRNLAELSREHFPRPVVW
                     GMWVVSEIAAMATELAEFIGGAIGISLLLHLPLIGSMAITALVTYAILTLQCTGFRPI
                     ELLIGGLIAVIGTSYFVELLVAHPNWPAVAYHSVVPHFDDTDAIMLASGIVGATVMPH
                     AIYLHSGLTQARIVARDNDERRKLIRFSNREVVAALGVAGVINMAMVVMAAAVFHDGL
                     HNGVAEIESAYRTLVPVLGIGAAGIFLLSLIASGVSSSVVGTMAGQVIMQGFVRRRIP
                     LWIRRLVTMAPAFVVVALGVNATHALIASQVVLSLTLPVPMFALLILTRRNDVMGSFA
                     NRRFVSMTVIGMAVLVLALNLLLIVQAVGTARAL"
     gene            complement(15866..>16301)
                     /locus_tag="VGA15_28590"
     CDS             complement(15866..>16301)
                     /locus_tag="VGA15_28590"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX9591689.1"
                     /translation="ARLAVPPDNQVQQIELLGKLMLYDKELSVNRNEACAFCHMPEAG
                     FTGRAAGSASAAALVARFDKSQHRRQGGAESTFLNLSREPARVADGGLSMIRKMSLVR
                     NSPRRASWQRRTREQIAGWRRRQGARRTAVSHLRERADAVVG"
BASE COUNT         3028 a         5175 c         4967 g         3131 t
ORIGIN      
        1 acgggtgtga aggtcttcaa ctggctcttc acgatgtatg gcggccgggt cgtctattcc
       61 gcatcgatat tgtggtctat cggcttcatg gtcaccttcg tgatcggcgg catgaccggc
      121 gtcctgctgg cagtaccccc ggcggacttc gtgctgcaca acagcctttt cctcgtggcc
      181 cacttccata acgtcatcat cggcggggtg ctgttcggcg cgttcgccgg ctacacctat
      241 tggtttccta aagcgttcgg cttccggctg cacgaaggtc tcggcaaggc cgcgttctgg
      301 tgctggataa ccggcttcta cgttgccttc atgccgctct acgttctggg cctcatggga
      361 atgacccgtc gcatgcagca ctacgatgtc cccgcctggc gtccatggtt gctgatcgcg
      421 gccggcggcg cagtcatcat tctggccgga ataatcctgc agatcgcaca actcgccgtc
      481 agcattcgca ggcgggaggc gctgcgtgac cagaccggcg atccctggga cgggcgctcg
      541 ctcgaatggg cgaccccctc acctccacca agcttcaact tcgcggtgct cccgcgcatc
      601 caaggcgagg aggcctactg ggccataaag cagcgcgcgc gccagcaggc gaagctgcgc
      661 gaggagtccg gctatacgga aatcgagatg ccgaggaata gcccaactgg cttcatttgc
      721 gccttcttcg ccaccttgat gggattcgcg ctgatctggc atatctggtg gatcgtggcc
      781 ctggccgcat tgggtgcgta cgcgaccttc gttgtcttcg cctggcggga taggcacgag
      841 tacatcattc cggctgatgt ggtcgcgcgc gtcgaccgag ccaaccgaag tgcacgcagc
      901 gaagcgctcg cccaactatg gacggcgcca tgagcggcct cgaggtgacc gccggtcgcg
      961 gcgatccgaa tcgcgccggg taccaccggg aactgcggga tcgccggcac ccggcaggcc
     1021 acgcggccgg tgcgctggtg tcgaagcgca tcatcgtcgg ttacggcttc tggattttcc
     1081 tgctcagcga catcatcatg ttctccgcct ttttcgcgac ctacgcggtg ctggtcgggc
     1141 agacggcggg cgggccgagc gggcgggagt tgttcgacct gcgcaatacc gggatcgaga
     1201 cggcctgcct actcctctcg agcttcgcct gcggcctcgc aagcatcggc gcccagacgc
     1261 accgcgggtc ctggttctat ggcgccatgg cggcgacctt catactcgga gccgcgttta
     1321 tcggcatcga ggtccgggag ttcgcgggct tggtcgttcg cggggcaggc ccaacacgca
     1381 gcgcgttcct ctcggccttc tttacgctgg tcggctgcca tggcctgcac gtgaccgctg
     1441 gcttgctctg gctgctgacc atggtggcgc aggtgttcgc caaaggttac cgggccgaca
     1501 ttctccggcg tgtcctctgc ttcagcctgt tctggcatgc cctggacatc gtttgggttg
     1561 cgctattcac gatggtgtac ttgatgggag ccgcggcatg agccactccg agcagaaaag
     1621 agactcgaat catgcggaca cggcgccggg cgacgagcgc ctcgacggac acgaggctgc
     1681 cgggggaata ggaacctacc tgattgggct tgggctcgcg acgctgctca ccatcgtgtc
     1741 tttctttgtc gcgcgcacca cgctcgtctg gcagccgagc attcccgtcg cactggccgt
     1801 tctcgcgatt gcgcagatgg gcgttcacct cgtcttcttc ctccacatca cgaccggccc
     1861 cgacaacgtc aacaacgtca tggcgcttgc cttcggcgtg ctgattgttt tgttggtcat
     1921 caccggttcg ctctggatca tggcacacat gaaccacaac atgatgccta tggatcagat
     1981 catgcagatg cagcgctgag ctgggctgag actccccgga acggcgggtg ggacaccttc
     2041 cgctcaatcg catgcgtaag cgcgcttccg cacgggtcgc ctgctctcgc ggcccagcca
     2101 cggtattccg gacatgccgc cgggcgaaat cgcctcggcc cagcggggct ccgctcagag
     2161 ccgcatcaga acgccacctg ggtgcgcatt gcgatcgcct ggatcacggc acccggtaac
     2221 gtcgagccac cgaccgcggt ggcggtgggg atcttgttca cggtggcgta gatatagttg
     2281 agcatgaagc ggatgttggt gatcggatac cagttgacgc ccaaggtgta gatcgtctgc
     2341 cggccgccaa agacgccgcc ggttgtcgtc ggcgccacac ccggcgtcgc gtggttattg
     2401 agattgacat agctgtagcg caccgcgagc tccaacgccc cccaaccgcc ggcggtgaag
     2461 gagagcggcc ggtcggggac gatggcgctg taggcgccgg tcgtcgggat atattttcgg
     2521 gactcgcccg tgacggtgta gctcgcctcg atgtagcccc cgtcgaaatt gagctgtggc
     2581 gtgggcgcgc ccgcggccaa gcccgcttga ttgacgaagt agcgataata ctccccctgc
     2641 gcgtagaagc tgccgaatcc gcccgctgct tcgaccccat acgcaccggc attctttgcc
     2701 gggatcgggc cggtgttgag aaagatggtg ggatcgacgc gcagctccgg gcgatcgctc
     2761 aacgtcatcg tgctggcgat cgacgtggct gagctgccat tggcccgagg cacgaagaca
     2821 tactcgccgt cgacgccgag atgaagcgaa tagttaccgc cttgaagcgc ttgataggtc
     2881 gcgcgcgcga gcccgcccag ctgctgctgg ttcgatcccg tatgagttgc accgctgagc
     2941 ggcccggtgg cataagcgcc ggcccaggca cgatcggtgt tccatcgcac gccggcggcc
     3001 gagcggaaat ccccggccgc cagcccggtc gccacgacct ctggtgaagc acgctcgagg
     3061 aacatgatat cgttcgtgct cgtcgcgtcg tcgagggccc agggaacgtc catgtaacca
     3121 atctcgaagg cgaagggcct caggccctga tagctgagcg acgcagtctc gatattcgat
     3181 agctgcgggc cgtctgcgga gccaccgaag tcggcaacga acgcatagtt ccaatcggcc
     3241 atgaacttgc cgagcacgcc gatgcgcgca cgccgggcat tgacgccgct ggtgaggctg
     3301 tgcagcccgg cgggcgtctg aggatggacg ctcagatagt cggcgacatc gaaatgcagg
     3361 cggccggtga gatagatgca gttcgcgtca tcggcgctgc agatgcccgg ccggttagcc
     3421 ggtgtcagca cggccttgac cccgctcggt ggtggcgcga ccggcgcggc ggccggcgcc
     3481 tgggctcgtc tcgactgctc ttcgacctgg ttctgcagat tttgcagttg ccgctggagc
     3541 tcgcggatct gctgctggag ctgctcatta gtttgcgcga cgctgggatc gaccgccagg
     3601 agcatcacac ccgccaatgc gctgccaaag agactgtact tcagtgtcat cgctggcccc
     3661 tttttccgga gcatcggcaa atgaaacgga tgcccctcct cggccgctcg gcggctatac
     3721 cgctatgcta cccacctacc gggctcaccg cggtgaagta gcgggccgcg acagcggcac
     3781 aaccggcggt aaacaacaat aattggcggc ggttcccact gaatctgcgc ccctgccgcc
     3841 ttcaacgcct gcaccgtccg tcctgccacc cgccgtgccc gacagcggcc gcgacggaca
     3901 ctgtcgcaca gctgcggaga tggtcttcca gatcgtgtcc gtagatctcc accgtcaaat
     3961 gccttggtcc aggtgcgatc aacgcaataa taggcacgta acggcaacgc caggcaagca
     4021 ttgtagctgt ttcgctcgat actcattgtc taaacccgct ttcgcgccaa gctgagacgg
     4081 gaacctggga gtcgcacatc acgacggttc ggcaattcgg gctcggcgaa agatgccttc
     4141 cagaggcgtt tctgcttaaa ctcggcagca ggcatatcag acggcgcggg atgcgttcca
     4201 cggtggcacg accataagga tgatgaccat gcgcgtattt ctcgtttcat tgacggccgc
     4261 cgttatcatc gcggtcggtg ccgctgctgt gctgtaccca tttcagaagc ctgcctctgt
     4321 cgcgttctct acctctgccg tacgcctctg aacgcggcat ctgatcggaa tcgctacgag
     4381 gacatcgcaa acgctttcgc ctcgatcgcc ataaataccc tgccgagcgc tccgatgctg
     4441 cggtagaact ccgcggtctt agccagttca agatccgcga agaatttctc catccaaggg
     4501 gcgagatacg cctcgaacaa cgcgcgatcc gctccgcgtg gagccaataa tcgtccgcct
     4561 acgagcccag ccatgatctc gcagagaatc gcggcatggt cttcgggctc ggcgcgcccg
     4621 ggcgctcgct cgatgccgag ccgggccagg tcgtagcgca ggcgcgcaag tggacgctcg
     4681 tgcagaaacc ccgtgagata gtacgagccg taaggcagaa gctcgccgcg accgagccca
     4741 atgaagagat cgaaatgctc gcgctcgacg ccctcccgcg ttgcgcacct ggcggcttcg
     4801 gcaagcgcgg catgggcaac gccgagtgga ctcgggtcac cgcaaagttc ggcaagccga
     4861 cggagtagat cggcgtcggg cgcgcgccca agcacggtcg ctagtagcgc atattcctgc
     4921 gctcgcgcta tatcgaccgc gtcgatatcg tcgatcgtga agtgacggtg ggagtaaaga
     4981 tccatgggaa attctctgaa tgaacgcaat ttaccgtacg aattttcacg cacagaccgg
     5041 tgaccagaat cactttggga gggcttgtcc gtgtctgcgc cggccaatta gtggcagctc
     5101 aggtttctcc cgctggtcaa tagttgcgtt cgttgctccg cgaagctgcg tcgccccgcg
     5161 gctgtccgca ttattctgcg gcgacgtgag gctgtcattg atcggctttc gtgggtcggg
     5221 tcgatcggga accgatccgc tcgatattgg cttggcctcc gtaatcttcg aaacacgggg
     5281 gttgccttcg gccgcgcccc ggccgatcaa atcagccgct tgtcggcgaa gcgcgtcagt
     5341 cggttccaac gacccaaagc cagccatgga acctggtgca ttgaagtccc agtcataatc
     5401 cgcgagccca acgaaatctc ggatcttgag gtcggtggtc cagacgcggc gtagcgcggc
     5461 gcgcgtcagc tcgggcggca cgaaaggcgc gagaaaattg cggatatcgg tctcggcggt
     5521 gatcgattcg atcgagggca gtttcgccag atcgaacgcc ggtccggtcg ggtcggcggt
     5581 ggtcttccgc gatatgggag catcgccaga ctgagcgcga gctttgcagt cgctctgctt
     5641 cggcccattt gcgcttgcgg cggtctcgcg cttgcggcgc gaccagcggg ccaaaaaatc
     5701 ttcacgcctg ctcattcgcg tcagtccctg gcttttggtc ggcggcgcgc cagcgcctct
     5761 gggtccgcag gattgcgttt gcgcttgtgg aacggctgct cgacgagatg agcagccacg
     5821 aaggcctcga cgctatcgcg cacggcagcc ggcatcgaca cggtagccac gaggtcccta
     5881 cccgtctgag taaaagattc gccctcggcc ggatctgccg tgactgctag aaggtcatag
     5941 ggcggctcga gcccggttgg cctcagtgcg atccacagca agggtacgtc acaggcgagg
     6001 ttgtcacggt aattgcgacc ttcggtccgg tacagcgcga tttcagttga acccacgtag
     6061 aaggttgcaa tcccggcatc aatagaaagg actgtccacg gcgccgcgtc cgggcgtccg
     6121 ggcagtgcca tgacgggctg ccaggtgaaa tcgatccacg gactcgtggc cctgcgccgc
     6181 tcgacgacga cgccaacagg gatccgtgca agcgcgggca cgacgctcat ctaacggtct
     6241 ccagctgcaa gagcttcaca tgcgcagccg tctcgtcgcc cttcatggtc gcggcggccc
     6301 gccgccggga cgaggtcatg tttcgccctt ttgcgtctgt tgttcgcgct cccgcagata
     6361 atcttcggtc gtgcgtatct tcgggcgggc cggcgcaccg gagcgatcga attccgcttc
     6421 ggccacggct atcacgcggc actcgccaca catcttcaat gcgtcgatcc gccggtcctt
     6481 cccctgatac atccaatgct tgcccgcgag cttggcggcg acccgctcaa tcgagctctt
     6541 cgtaccgaat ggcgtaccgc aacgtatgca gtgaaacggc tcctcttgct tgagcacgcg
     6601 tgcgggtcca agggtcgcga gaatgtcgag ctgcggcttg agcgtgatga ccttctcggg
     6661 gcaggtcgcc ttacacaatc cgcactgcac gcaagcgtct tccatgaaac ggagcatggg
     6721 ccgctccgga tcgtccaaca acgcgccggt cgggcaagcc gacacgcaag caaggcataa
     6781 ggtgcatcct tccacattga cttcgaccgc gccgaaaggc gctccttcag gcagcgcgat
     6841 tacttcggcc gatgccggcg ccgcagcacg caactcgcgc agcgccatcc gcagcacgtc
     6901 ccgcttccct ccgatgggca ggaaggtcgc gggcctggga gcgggatcag gtgcggcaat
     6961 cgcccgcagc atctctccca gcatgtccgg atcgtcggta tcgactgtcg ccgcccgacc
     7021 cggcccgaac ccaaagccga ccaggatggg ctccgcgagc gcgagcgtcg tcgcaaggct
     7081 cgcgacgtca tggcgtggct ttgcgcgcag caggagacgc agcgccgatg cgccataagc
     7141 gaaagctgcg gcgattactt ccagacctac ctgcgtgacc tcgttgaccg cgagcggcag
     7201 cacgttcgcc ggcagcccgt caccgaaacg cgcgagcgca tctatcaagg gcgtgccatg
     7261 cgcctcgtcg tgcagcaata cgatcgcgcg cttgccgccc gcatcacgat aggcggtcag
     7321 caatgcgcgc aatcgccgca tcagcgcgtc ggccggcggc agcgcatagg aggcggcacc
     7381 tgtcgggcag acggaagcgc attgtccgca tccagcgcaa atctgcgcat cgatggctac
     7441 gtgatcgccc gccggcctga tcgcgccggt ggggcagagg ttgaggcagc gattgcaccc
     7501 gacgatctga gagcgcgagt gagcacagag atcgtcggcg aaagtaatgt aacgcggctt
     7561 ttcgaaactg ccgacgaggt cccttgccct gagcacggcc tttaccaccg cggccggact
     7621 gcccggatcc gcccgcaaat agccatcgcg cagatcggcc gccgggaaga gcggcgcgcc
     7681 gcccgatagg tcaagcacga tgtcgcaacg tgacgtcgcc ccatctttgg tcggaccgaa
     7741 ggccagcacg ccgcgcgatg acggcgaggc ttgaccaaat ttgtcgacgg taagctcgaa
     7801 ggcgccaaga tggcctttgg ccgagcggat ttcccccttc gccactggaa agtcggtcac
     7861 cgctggcggc tgcagagcag cgggtggctt gatgagcacc gtcacatcga gatgctgctt
     7921 gagcagattg gcggcttcga tcgcttgttc gtcacgccca taaacgaggg tcacgccctc
     7981 gctgttgaat ttgacgacgg aaatttccgg cgccgcttcg gctgctgctg caatcagcgc
     8041 tgccatcttc gcgcttgcct tcgctgcctc cgcggaccaa cccgcagtct cacgtatatt
     8101 cgcgtaacgg atatcggcac cgctttcgtg cccttcggca acctcggaaa agagaggcgc
     8161 ttcctgcgtg caggcgacga tcagaggacc cccgccggcc gccgcggccc ggaatcgctc
     8221 gagttcagtg cggcagagct gacgcgccgt cgtcacctga ctgccccgac agccgcgccg
     8281 caccgcttcg gcatctagcg gcatcgtgtc ttcgcacgag caaatcaaaa tacgttgccg
     8341 ctgatccgtc atgctgactc cggtgcgcgg ggtcttcggt cgatcgttca ctcggccggt
     8401 gaagtcgagg cctctggcgc cggggtgggc ccccgccctt ttgcgatttc ttcttcgagc
     8461 caaatgcgat gatgctcctc ggcccagttg agatcgacgc tgccggtacc catggcttcg
     8521 aacgcccctt ccatcccgag agtaccgatg tagatgtgac cgaggatgac cgcgatgaag
     8581 aaaacggcaa tcaagctgtg cacgatttgg gcgagttgca ttccggcaat gttcgtcaca
     8641 tagaacggga acagcagcag atatccggat agcgccacgg ctgcgccgcc accggcagcg
     8701 aaccagaaga ccagtttttc tccggcgttg aatcggcccg cttgcgggcg cttcgagccg
     8761 acaaaacccc cgccttcctt cagccaaacg atgtcgattt ttcgtggaat gttgtcctta
     8821 atccaaagtg ccacgatcag gacaagcccg gcgacgaatg gaaagctgac gaaattgtgc
     8881 acgtatttgg ctgcctccga aacacggctg aaggcatccg ggccgagtaa tggcagcagc
     8941 aacagctttc cgaatgtgat gttcagcccg gtcagggcta gaacgacgaa cgacgcggcc
     9001 gtgagccagt gcgaaaagcg ctcgaaggcg ttgaaacggc gcaccttccg tccggaacgt
     9061 ccggctgaga tcctgatccg tccgatgcta agataggcaa tcgccagcag ggcaatcgca
     9121 ccgacgatca cgacggtgcc gatccaatgc aggagcactt catggaagaa gcgccagcct
     9181 ctgccagccg gctgtatcag gacacgctca cgcggttcgg gtatgacgat tgtcccctcg
     9241 atccgcggcg attctcgcag caggttctgt tcctttaccg cgctcgcatc tggatcgacc
     9301 aagggcgctt gcgctgctga tggtgtctgt ggctgggatt gaggctgctg cgcgtttacc
     9361 ggagccgcga tcagggcaat accgaggaag atgaaggcac aaatcaaggt gcacatggcg
     9421 agcggagtgc gcatgcaatg ggttgccatg ccttgtcctt tcactgtttt ctcactcaac
     9481 gccgaccgcc tgctaccctg tggcgctgtt gcgctaaatg cccgtgtcgg caccgtaggc
     9541 ggtggtccaa ccccaggcgc cagagccgta gccgcgcgtt tcgacgcgct ctttgtagat
     9601 gccagcgacg gtttcgccgt cgcccgcgag gagtgacttg gtcgagcaca tctcggcgca
     9661 taacggcagc ttgccttcgg cgaggcggtt ggcgccatat ttggcgtatt cttcgggcga
     9721 gttatgcacc tccggcccgc cggcgcaaaa agtgcattta tccatcttgc cgcgcgagcc
     9781 gaaattgctc acttggggat attgcggcgc accgaatggg cacgcgtaga aacaataacc
     9841 gcagccaatg cactgatcct tggaatgcag caccacgcca tccgcggtcg tgtaaatgca
     9901 ctcgaccgga caaaccgctt ggcagggcgc gtcggtgcag tgcatacagg ccatggacac
     9961 cgaacgctcg ccaggcttgc cgtcattgat cgtgacaacg cgccggcgat tgagccccca
    10021 cggcacctcg ttttcgttct tgcacgcggt cgcgcaggcg ttgcactcga tgcaacgatc
    10081 ggcgtcgcaa agaaacttca tcctcgccat ggcgccgctc cttaagccag cgtaatctga
    10141 cagagggtga ccttgggttc ctgcatgttg gtcaccggat cgtatccgta ggtcgtcacc
    10201 gtgttggcgc tttcaccgag cacaaacgga tcggcgcctt gcgggtattt gcggcgctga
    10261 tcgactcctt ggaaccagcc ggcgaaatgg tacggcatcc atgccacccc cttgccgacg
    10321 cgttcagtga caagcgcttt catgcgcgct ttcgaagcgt attccgcgcc ggtcacccaa
    10381 acccaagcgc catccttgat cccgcgctcg atggcatccg ccgggctgat ctcgacgtac
    10441 atgtcctggc gcaactcggc gagccaccgg ttggaccggg tttcctcgcc accgccttcg
    10501 tgatcgacga gccggccgga gctgaggatg agcgggaatt gtttcgcgac gccccggtcg
    10561 acagcgctct tctgaaccgt gaagccgata ttcggcacgc ggaactgctt ggcatccggc
    10621 agcgtcggat attcgccgac gagatcgaca cgcggtgagt agatcggctc gcgatggacc
    10681 ggcaccggat cgggcagatt ccacgccacc gcacgggcct tgccgttgcc gtgagggacg
    10741 caaccatgtt caattgccac gcgctgaatc ccaccggaca ggtcggtcga ccacgccacc
    10801 gcatcggtat tgttgccgcc gactttcgct atgacggcca gctcctgcgg cgtaagatcc
    10861 ttgtcccagc caagcttttt gagcacggcc atcgtgaact cgggatagcc gtctcggatt
    10921 tccgagccgg ccgagtatga gccttcggcg agcaacgtga cgccattgcg ctccaccccg
    10981 aagcgcgcgc ggaaggtacc gccaccatcc ttcacgtgga ggcttgtgtt atagagcgtg
    11041 ggcgtcccag ggtgcttcaa ttcgggcgtg ccccagcacg gccatggcag gccgtaatat
    11101 tcacccttgc aagggccggc gctcgcgcgc aacgtgacca gatcgaagtc cttttgatgg
    11161 tcgcggtgca gcttgagccg ttccggagat tggccggtat atccggtgct ccagccgccg
    11221 cgattgatct cgcgcagcag atcctcggca gaaggcaagt tgttcaccac cttgatgttc
    11281 ttgaacagtt gatccgcgaa gcccagcttc cgggcaagca ggtagatggt ttcgtagtcc
    11341 tccttcgcct cgaaaatcgg agcgacgacc cggtcgtacc attgtagaga gcggttggac
    11401 gccgtacgtg tcccggagga ttcgaactgt gtgccggagg gcagcagata gacgccgtcc
    11461 ttgcgttccg agagcaccgc ccaggtcgtc ggaacggtat cgacgacgac cagcagatcg
    11521 agcgcttcga tgccttttgc ggcttccggc atccgtgtga tggtattgcc tgcatggccg
    11581 aagaccatca tggcgcgcaa attgtccggc tgatcgatct gatcctttgc cagcagagtc
    11641 gcgtcgaacc accgcgtcga cggaatgcct gctgtctcca tcatcttctt tgagatgaag
    11701 cgcgattgca cccagtcgta gtcgatgtcc catacccggc accaatgtct ccaggccccc
    11761 tcatcgagcc cgtaatagaa cggcagggta gtgacgtcga gcccgacatc cgttgcgccc
    11821 tgcacattgt cgtgaccgcg gaaaatgttt gcgccgttgc cgaatgcgcc aacattgccg
    11881 gtgacaagca gtgcgataca gctggctcgg acgtttgccg tcccgacggt atgctgcgtc
    11941 tggcccatgc accaaatcag cgtcgccggt ttctccttgg caaacttttc cgcgatacgc
    12001 ttcaactgct cgccgggaac tccgcttacc cgttcgacct cttcgggcgg ccacttcgcc
    12061 acttcccggc gaatctcgtc gatgccatag acacgctggc ggataaactc cttgtcctcc
    12121 cagccgttct ggaaaatgtg ccacaacatg ccccacagca ccgggatatc tgtccctggc
    12181 cggatacgga cgtagtccgt tgcatgggca gcagtgcgcg tcatacgcgg gtcgatgaca
    12241 atgaagttcg cccgattgag ctccttgcct tcgagcagat gttgcaagga taccggatgt
    12301 gcctcggcgg gattgccgcc catgatgagc agcgtcttgg aattgcggat gtcattgaag
    12361 ctgttggtca tggcgccgta gccccaggta ttggccacgc ccgcgacggt ggtggaatga
    12421 cagatccgtg cctggtgatc agcgttgttg gttccccaga acgctgcgaa cttgcggaag
    12481 agatacgcgc attcattcga aaactttgca gagccgagcc aatagacgga ttcgggcccg
    12541 gaggtttggc ggatcgccat cagcctgtcg ccgatctcgc cgatggcctg atcccaggaa
    12601 atgcgcaccc acttcccgtc cacgagcttc gtcggatatt tgaggcgccg ttcgccactc
    12661 acgagctcgc gcaccgacgc gcccttggag caatgcgatc cgcgattgat agggctgtcc
    12721 cagctcggct cttggccaag ccagacaccg ttttgcacct cggccgtaac cgtgcagcca
    12781 accgagcaat gcgtgcagat gctcttgcgc gtctcgaccg ggattcccga ttttggaggc
    12841 gggccggctt ccgcgttgcg gacgccacta accggcagcg tgccgatcgc ggcaagagcg
    12901 ccggctgcaa gaccggagcg gcgaaggaac gcgcggcggt cgatcccgcc agactccgcc
    12961 gcgagtgcac cgaccaaggt gccgcggcga gcagcgcgtt ccgctctctt gatgagcatc
    13021 agcgcctccg ttcagtcgga tagcggttga cccggtaaaa ggtcttgacc tcttcggtct
    13081 ctcgataaag aggtttgcgt cgttcgtcgt agctcaatgt gtcggctacg gcatggcccc
    13141 cgagcggtgc cgtcgaagct gcggcagccc agccgcccgc cgccaagacg cggaggaagc
    13201 tgcgccgacg aaacactatc ttgtcgtcag catccatctg ccgttcgtcc gtcgaggcct
    13261 cctacgtcgg ctcgaaattg aggtcagcgg tggccgagca tgccttgtca accgggcggc
    13321 tcttcctgtc gcatcatttg gatcgtccgc tttttgctca gatcggaaat cggtcggtcc
    13381 tgaacaagca aaactcgtat gatcgaacca tgcccaaaga cccgccaatt gaattgaccg
    13441 acattgctac gattggctcc gcctttgcgt cgctttccgt aaacaaggta ttcggctgga
    13501 tgaggctgct gtcgcggctg aagccatggg aggccgcatc ggtacgcacc tatcggttcg
    13561 gcggcaagaa cgacgagcgt accttattga acccgcagga ggttgtggcg aacggataac
    13621 gcagcttaga tcgattgcgt agggtgaacg cgcatcgtca gcccgatctc atccgattcc
    13681 tctcgcgcat cccggtcatt gccgagggct atccagttcc ggcagcgaaa ggcggagctt
    13741 gtccttggca gcaaagttct gcgcgatcgc ttaaacgagt ttaaccgcgg cgtgactttc
    13801 ggcgatagga cttccttcct cggaggggat ttttcgctac gcctaaacca aggtgtgatc
    13861 tccatcgtcc tcggcggtgt ggaacttctt acacaagtgc aatagatcga gctgagacac
    13921 gtcgctagcc tgaagcaact gctttggaag gtagtcccct cagttaccac accctacctg
    13981 cctacccgtc aatcgagaga ggaacggatg gctgcactca tcgttaggga gaacccccag
    14041 cttcgtcgga tcgagcgtgc tattgccgag ctcgaagcgc gccctgcagc ggaacaaatg
    14101 cgcaaacaga ccctgctgac cgccctatac acggaacgag ccgagatcgt acgcactcgt
    14161 tcgcagtaac gtcgcgatta gcctgtgacc tgatcgtcct tcggagtccc tgcaaggaat
    14221 agccccgccc tcaccggggc agcggactga tcgccgcaag cgagcgacct gccttcgaca
    14281 cgatgccgcg atgcgggacg ggtgttattt gtcgcggcgc catcattgct tgctgcatcc
    14341 gcccgggagg ggagcagcag gcccattcct ccgacatggg cgaggctaag gcccgttatc
    14401 cgaactgcac gtcgccggag tttcggcgac cagtgcccac ccggctctgg cgttcagctc
    14461 agcccgattc ccggcgagag cggtgttaaa gcgcgagcag tggtctcagc ggtggcggcg
    14521 gtcaccgacg gtcgtggccg caaagggcgt cgccaatagg acccgcctct ggtttcgcct
    14581 cctacaatgc ccgcgcggtg cccaccgcct gaacgatcag cagcaagttc agcgcgagca
    14641 ccagaaccgc catcccaatc actgtcatcg agacgaagcg ccggtttgca aacgacccca
    14701 taacgtcgtt acgccgcgtg aggatcaaga gagcgaacat tgggacgggc agcgtgaggc
    14761 tgagcacgac ttgactggcg ataagcgcgt gcgtggcgtt taccccgagc gctacgacga
    14821 cgaatgccgg tgccatcgtc acgagccgtc ggatccagag cgggatccgc cgccggacga
    14881 atccctgcat tatgacctga cccgccatcg tgcccaccac cgagctggaa acacccgacg
    14941 caatcaagga cagcagaaat atgcccgcag cgccaatgcc gaggaccggc accagtgtgc
    15001 gataagcgct ctcgatctcg gccacgccat tgtgcaagcc gtcgtgaaag acggccgccg
    15061 ccataaccac catggccatg ttgatgacgc cagcaacgcc aagggccgcc acgacttcgc
    15121 ggttcgagaa gcggatcagc ttgcgccgct cgtcattgtc tcgcgcgaca atacgggcct
    15181 gcgtcaggcc cgagtgcaag tagattgcat gtggcataac cgttgcgccg acaatgcccg
    15241 atgccagcat gatggcgtcg gtatcgtcga aatgcgggac gaccgagtgg tatgcgacgg
    15301 ctggccagtt cggatgggcg acgagaagtt caacgaaata actggtgccg atcacggcga
    15361 tcaggccacc gataagcagc tcgatcggcc gaaacccggt gcactgtagc gtcaggatcg
    15421 cgtaagtcac gagggccgtg atcgccatgc tgccgatcag cggcagatgc agcagcaacg
    15481 atataccgat cgcaccgccg ataaattcgg caagctccgt tgccatcgcc gctatctcgc
    15541 tcacgaccca catcccccat acgacgggcc gcgggaagtg ctcacgcgac agttcggcca
    15601 ggttgcggcc ggtgacaatg ccgagcttgg ccgacagcgc ctggaacagc atcgcgaccg
    15661 cgttggcgaa aacgactacc cagagcaggt tgtagctgta cttggcaccc gcctcgatat
    15721 tggtcgcaaa atttccgggg tccatatagg cgaccgaagc gacgatcgcg ggaccggcga
    15781 agggcaggat tgaccgcatc ccgccacccc gcccggctag tgagtcgcgg ccggcaagga
    15841 tcaggcgatc cgtcgcagtc attgattagc ccacgacagc gtcggcgcgc tctctaaggt
    15901 ggctcaccgc cgtgcgacgc gctccttgcc gcctacgcca accggcaatt tgctcacgcg
    15961 ttcttcgctg ccacgatgcg cgtcgcgggc tgttcctgac gagagacatc ttgcgaatca
    16021 tcgacagccc tccatcagca acacgagccg gttccctgct aagatttaag aacgtcgact
    16081 ccgcgccgcc ttgccttcga tgctgggact tatcaaatct tgctacaagg gcggccgcag
    16141 acgctgatcc tgccgcccgt ccggtaaagc ccgcctcggg catgtggcag aaagcacaag
    16201 cctcgttgcg gttcaccgag agctccttgt catacagcat cagcttgccg agcagctcga
    16261 tttgctggac ctgattgtcc ggcggcacgg ccagccgcgc g
//
LOCUS       DASWGM010001062         2505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_2759983,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001062 DASWGM010000000
VERSION     DASWGM010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2759983"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VGA15_28595"
     CDS             <1..663
                     /locus_tag="VGA15_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HEX9591690.1"
                     /translation="MGGRIGYSAATGIMVVLLSWFGIISVLLALVPVVAISPILLYIG
                     MLIGAQAFQTTPIKHAPAIVLALTPHLAAWAKLQIDTMLGATLNAAQSVGALAPDKVG
                     AVKTAAIASLPQQGVLYHGLEVMGGGSILAGLILGAIGVFVIERDFAKAAAFAFAGAV
                     LTYFGFMHGEAVGTGGGLGVTPGVALAYAVVAVGFLAVEKFGASTDYVSHPDTPLAAP
                     AE"
     gene            complement(737..2035)
                     /gene="glnT"
                     /locus_tag="VGA15_28600"
     CDS             complement(737..2035)
                     /gene="glnT"
                     /locus_tag="VGA15_28600"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HEX9591691.1"
                     /translation="MTIDLVQAAKEKGIRYFLISFTDLMGTQRAKLVPAEAIGQMVKS
                     GAGFAGFATWLDMTPADPDMFAIPDPDSLVQLPWKPEVGWLAADPWMNGKPVEQAPRN
                     LLKRLKAKAAESGMQLKSGVECEFFLISTDGTGIKDSADRQAKPCYDQSALMRRYDVI
                     SEICDGMLKLGWKPYQNDHEDANGQFEMNWEYDDALVTADRHAFFKYMVKSIAEKHEM
                     RATFMPKPFSHLTGNGCHMHVSLWKGAQNLFEDEQGELGVSALGYHFIGGIIHSADAL
                     AAILNPAVNSYKRINAPRTLSGATWSPNSVTHSGNNRTHMIRIPEGGRFELRLADGAA
                     NPYAMPAAILAAGLDGINAKRDPGKRLDINMYTDGHLAGEVKRLPLNLLDALRALQAS
                     KLLNESLGEYVPAYLKLKHQEWDDFSRHLTDWERDATLDC"
     gene            complement(2068..>2505)
                     /locus_tag="VGA15_28605"
     CDS             complement(2068..>2505)
                     /locus_tag="VGA15_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX9591692.1"
                     /translation="LIVSGGIRNGADVAKALALGADAVAIGTAALIALGDNNPQLEAE
                     YQALGTTAGAYDDWHEGRDPAGITTQDPELAKRLDPVLGGRRLANFLSVLTLETQTIA
                     RACGKSHVHNLEPEDLVALTVEAAAIARVPLVGTDWIPGQHRY"
BASE COUNT          407 a          758 c          821 g          519 t
ORIGIN      
        1 atgggcggcc ggatcggcta ttcggccgcg accggcatca tggtggtgtt gctcagttgg
       61 ttcggcatca tctcggtctt gctggcgctg gtgccggtgg tcgcgatctc gccgatcctg
      121 ctctatatcg gcatgctgat cggcgcccag gcgttccaga ccacgccgat caagcatgcg
      181 ccggcgatcg tgcttgctct gacgccgcat ctggcggcat gggccaagct gcagatcgac
      241 accatgctgg gagcgacgct taacgcggcc cagagcgtag gcgcgctggc gcccgacaag
      301 gtgggcgcgg tcaagaccgc ggcgatcgcg tcgctgccgc agcaaggcgt gctttatcac
      361 gggctggaag tgatgggcgg cggctcgatc ctggccggcc tcatcctcgg cgccataggc
      421 gtcttcgtga tcgagcgcga ttttgccaag gctgcggcgt tcgcctttgc cggcgccgtg
      481 ctcacttatt tcggcttcat gcatggcgag gcggtcggca ccggcggcgg cttgggcgta
      541 acgccgggtg tcgcgctggc ttatgcggtg gtggcagtcg gatttctcgc ggtcgaaaag
      601 ttcggtgcca gcaccgatta cgtttcgcat ccggatacac ccctcgcggc gcccgccgaa
      661 tgatgcgagg cgaaggtttg gcgtcgcata ggatcagatg gccgggattt ttcccggcca
      721 tcaacgtcat cgtaccctag caatcgaggg tggcgtcgcg ctcccagtcg gtcagatgcc
      781 gcgaaaaatc atcccattcc tggtgcttca gtttgagata ggccggcaca tactcgccaa
      841 gactttcatt caggagtttt gaggcctgca gcgccctcag tgcgtcgagc aaattgagcg
      901 gcaggcgttt gacctcaccg gccagatgcc cgtcggtata catgttgatg tcgaggcgct
      961 tgcccgggtc gcgtttggcg ttgatgccgt ccaggcccgc cgccaggatc gccgccggca
     1021 tggcgtaggg attggcggcg ccgtcggcga ggcgaagttc aaatcgtccg ccttccggaa
     1081 tacgaatcat gtgggtgcgg ttgttgcccg aatgggtcac tgaattcggc gaccaggtcg
     1141 cgcccgacag cgtccgcggc gcgttgatcc gtttgtagga attgaccgcc ggattgagga
     1201 tcgcggccag cgcgtcggcg gaatgaatga tgccgccgat gaagtgatag ccgagcgcgg
     1261 agacgccgag ctcgccttgc tcatcctcga aaaggttctg ggcgcccttc cacagcgaca
     1321 catgcatgtg gcatccattg ccggtcaaat gcgagaacgg ctttggcatg aaggtcgcgc
     1381 gcatctcgtg cttttccgcg atcgacttca ccatatattt gaagaacgcg tggcgatccg
     1441 ccgtgacgag ggcgtcatca tattcccagt tcatttcgaa ctgaccgttg gcgtcttcat
     1501 ggtcgttctg gtacggcttc cagccgagct tgagcatgcc gtcgcagatc tcgctgatga
     1561 cgtcatagcg ccgcatcagc gcggactggt cgtaacaagg cttggcctgc cggtcggcgc
     1621 tatccttgat gccggtgcca tcagtcgaga tcaggaagaa ttcgcattcc acgccgctct
     1681 tgagctgcat gccgctttcg gcggcctttg ccttcagtct cttgagcagg tttcgcggcg
     1741 cctgttcgac cggcttgccg ttcatccagg ggtcggcggc aagccagccg acttccggct
     1801 tccagggcag ctgaacgagg ctgtccggat cgggaatggc gaacatgtcg ggatccgccg
     1861 gcgtcatgtc gagccaggtc gcaaaccctg cgaagccggc gccgcttttc accatctggc
     1921 caatcgcctc ggccggcacc agcttggcac gttgcgtacc catcaggtca gtgaacgaaa
     1981 tcaggaagta tctgatgccc ttttctttgg ccgcctgcac gagatcgatt gtcatggggt
     2041 cctcttgatg accatgggaa gggagcgtca gtatcgatgt tgacccggga tccaatcggt
     2101 gccgaccagc gggacgcggg cgatcgcggc ggcttccacc gtgagcgcga cgagatcttc
     2161 cggttcaaga ttgtggacgt gactcttgcc acaggcgcgg gcgatggtct gggtttccag
     2221 cgtcagcacc gacagaaaat tggccaatcg ccggccgccg agcaccggat cgagccgctt
     2281 ggccagttcg ggatcctgcg tcgtgatccc tgcgggatcg cggccttcgt gccagtcgtc
     2341 ataggcgccg gcggtggtgc cgagcgcctg gtattccgcc tccagctgcg gattgttgtc
     2401 gccgagcgcg atcagcgccg cggtgccgat ggcgacggca tcggcgccaa gcgccagcgc
     2461 cttggccacg tcggcgccat tgcggatgcc gccggagacg atcag
//
LOCUS       DASWGM010001063         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1515251,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001063 DASWGM010000000
VERSION     DASWGM010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515251"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..475)
                     /locus_tag="VGA15_28610"
                     /pseudo
     CDS             complement(1..475)
                     /locus_tag="VGA15_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612364.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(641..1148)
                     /locus_tag="VGA15_28615"
                     /pseudo
     CDS             complement(641..1148)
                     /locus_tag="VGA15_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            1255..>1561
                     /locus_tag="VGA15_28620"
     CDS             1255..>1561
                     /locus_tag="VGA15_28620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591693.1"
                     /translation="MFLPLAKTSCDGTGNIAKNATAGPPLHLTRILAVAAYVRCVTAG
                     VDPRLKLALSRAAQRHVFALWVFASGAIGRRLPRIALMGYQLVAALPGGRMHRLLTTL
                     "
BASE COUNT          290 a          449 c          481 g          341 t
ORIGIN      
        1 cgcgcgaccg cgctgttttt cgtccgggta agcgtggctg agcagcgcaa gggagttggg
       61 aacgagaatg gcggcaccga gtccctggac cgctcttgcc gcgatcagga tcgcggcatt
      121 gggcgcgagc gcacaggcca ccgatgctgc cgtgaacacc gcaaaacccg ccatgaagat
      181 tcgcttggcg ccgagccgat cgccgagcgc cccggccgtc agaatgaagg cggcgaaggc
      241 gatggtgtag gagctgacaa cccactgcag ctcggaaaac gccgccgccc agcgaggcgc
      301 cgatgctgtt gagcgcggtg ttgacgatgg tgacgtcgag ctggaccacg ccgtagccaa
      361 ggctcatggc caccagcgtc agcacagttg cgaggttcga gcgagccgat cgggcatccg
      421 gccgggagct tgcgtcggct ggaaacgact ggaatttcaa ggaagtactt cgcatggatc
      481 acctcaattt tgagggacca gcgcaagaac tggtgctccg tttcatcaaa acgtttcggc
      541 cgtgatcgaa gtattgtcgc ggatccggcc tgttcaacgc gaaacgccgc gcgaaagatt
      601 ccggcgcggc gttttgtctc agttcatgct caacttaaga tcagcccgcg gcgcgaaggt
      661 tgatcttgct ctcggccagc gtggtcaact ttttgtcggt ggccctttcc tcatcgaggg
      721 tcttctgcgg gacactggcg caatcgttgc ggccaagctg cttcgcccac gcgatcaggc
      781 tgccgtagcg cgtgatctca taatgctcgg cggcctgggc ggcgttgatc aatgccgcat
      841 ccagcaccga tttgtcctcg acctcaccga tgacctcgtc ggcttcctcg atgatgccgt
      901 cgatcgccgg acagtcgact gccttcactt cggcgccatg catcttgaac acctgctcaa
      961 ggcgctgcag atgggttttg gttttcatcg agatgggtca gaaaaccctg cttgagctgc
     1021 ttgtcggttg ccttttccgc cattttcggc agcgccttca cgagctgcat ctcggcataa
     1081 tagatgtcct gcaactggtg cacgaacagg tcgttcatgg ttctgatgtc tttggtaaag
     1141 agtcccattg cgcggaatcc tttttatttg gggaacacac agcgaagcct ttgtcgtggc
     1201 cgctgcgccg atatgttcga ttgctgatgc cgcgctaacc ggcggatccc ggggttgttc
     1261 ctgccgcttg cgaaaacgtc gtgcgatgga acagggaaca tcgcgaaaaa tgcaacggcc
     1321 ggaccgccgc tgcacttgac tcgaattttg gccgttgcgg catatgtacg gtgtgtgaca
     1381 gccggcgtcg atccaaggct gaagctggcg ttgtcaaggg ctgcacaacg acacgttttt
     1441 gccttatggg tctttgcaag tggagcgatt ggccgccgtt tgccgaggat cgctttgatg
     1501 ggctaccaac ttgttgccgc cctgccggga ggacgaatgc accgactgct gaccacgctt
     1561 g
//
LOCUS       DASWGM010001064         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1653500,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001064 DASWGM010000000
VERSION     DASWGM010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653500"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VGA15_28625"
     CDS             <1..684
                     /locus_tag="VGA15_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX9591694.1"
                     /translation="NGTKIWTSSAQVANYMLAIFRTSPPTKENRRHGLTQFLVDMKTP
                     GIKINPIRQLTGKSEFNEVVFQDAFVADDHMLGEIDGAWKQATSELAYERSGPERFLE
                     TYYVLTELVRALGEEPDNRGAEGIGRLVAQLHTMRRMSVSVAGMLHAGKEPVVEASIV
                     KDIGTIWEQQLPQRVRDLAAFVEPDASNHEILDTVLPYAMKVAPKLTIQGGTTEVLRG
                     IIARGLGLR"
     gene            977..>1768
                     /locus_tag="VGA15_28630"
     CDS             977..>1768
                     /locus_tag="VGA15_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX9591695.1"
                     /translation="MTKFTDIGVEKVGHVGTIEIQRPPLNFFDISLINQIADALEEFD
                     RDIEIRASVLAAQGKAFCAGANFNDPARQEQEARVAKGDPADGLGPINHLYVQAVRIF
                     RNKKPIVAAVHGAAIGGGLGLAVSADFRVTCPEARFAANFTKLGFHPGFGLTATLPEL
                     VGKNNAELIFYTSRRVTGEEATRMGLANVCVPQDQVRAEAMKLAQEIAECSPLGLLAT
                     RATMRAGLADRVMAATNHELAEQTRLRATEDFKEGIKATEERRVAN"
BASE COUNT          381 a          553 c          543 g          291 t
ORIGIN      
        1 aacggcacca agatatggac ctcctccgcg caggtcgcca actacatgct ggcgatcttc
       61 cgcacctcgc cgccgacaaa ggaaaaccgc cggcacgggc tgacgcaatt tctcgtcgac
      121 atgaaaacgc cgggcatcaa gatcaatccg atccggcaac tgaccggcaa atcggagttc
      181 aacgaggtcg tattccagga cgccttcgtg gctgacgacc acatgctcgg cgagatcgac
      241 ggcgcctgga agcaggcgac ctctgagctt gcctatgagc gcagcgggcc ggagcgcttt
      301 ctggaaacat actacgtgct gaccgaactg gttcgcgcgc tgggcgagga acccgacaat
      361 cgcggtgccg agggaatcgg acggctggtt gcgcagcttc acaccatgcg ccgcatgtcg
      421 gtgtcggtcg ccggcatgct gcacgccgga aaggaacctg tggtggaagc ctccatcgtc
      481 aaggacatcg gcacgatctg ggaacagcaa ttgccgcagc gggtgcgtga tctcgcagcg
      541 ttcgtcgagc cggacgccag caatcacgaa attctcgaca ccgtattgcc ctacgcgatg
      601 aaggtcgcac cgaagctcac catccagggc ggcaccaccg aggtgctgcg cggcatcatc
      661 gcgcgcgggc tcggcctgcg ctaggcgcgc gggcattgag ttttgaaaga gcgtgccgcg
      721 cgctcgactt acctctccca ttgggagagg tcgacgttcg agcgaagcga gaaggtcggg
      781 tgaggggtta cgacccatcg ataggccgta acccctcacc ccaaccctct cccatcgaag
      841 tcggatatat ccgacttcga caaatcaagg atgctcaact cgggtaaacc cgagttgagg
      901 gggagaggga gcgcgttttc actgcggcga aagcttcggc aaactatgat tcataacgaa
      961 tagtggagac cacatcatga caaaattcac agacatcggt gtcgaaaaag tcgggcatgt
     1021 cggcaccatc gaaatccagc ggccgccact gaacttcttc gatatctccc tgatcaacca
     1081 gattgccgat gcgctggaag aattcgatcg cgatatcgaa atccgcgcgt cggtgctggc
     1141 cgcccagggc aaggcgttct gcgccggcgc caatttcaac gatccggcgc ggcaggagca
     1201 ggaggcacgg gtggccaagg gcgatccagc ggacggtctc ggcccgatca accacctcta
     1261 cgtccaggcg gtgcggatct tccgcaacaa gaagccgatc gtcgcggcgg tccatggcgc
     1321 ggccatcggc ggcgggctgg ggctggcggt atcggcggat tttcgcgtca cctgccccga
     1381 ggcgcggttc gccgccaatt ttaccaagct cggctttcat ccgggcttcg gactgaccgc
     1441 gacgcttccc gaactggtag gcaagaacaa tgcggaattg attttctaca ccagccgccg
     1501 ggtgaccggc gaggaagcca ccagaatggg cctcgccaat gtctgcgtgc cgcaggatca
     1561 ggtgcgcgcc gaggccatga agctggcgca ggagattgcc gaatgttcgc cgctcggcct
     1621 gctcgcaacc cgcgccacca tgcgcgccgg cctcgccgac cgcgtgatgg ccgcgaccaa
     1681 tcacgaactc gccgaacaaa caaggctgcg cgccaccgaa gacttcaagg aaggcatcaa
     1741 ggccaccgaa gaacgccgcg tcgcgaat
//
LOCUS       DASWGM010001065         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_274729,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001065 DASWGM010000000
VERSION     DASWGM010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274729"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VGA15_28635"
     CDS             complement(<1..286)
                     /locus_tag="VGA15_28635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9591696.1"
                     /translation="MAEALAQFAQTEFPQARTAQPRVAVLVPCFNEEAAVATVVTDFR
                     KALPTAEIFVYDNNSSDRTMAVAREAGAEVRSERRQGKGHVVRRMFADIDA"
     gene            complement(410..895)
                     /locus_tag="VGA15_28640"
     CDS             complement(410..895)
                     /locus_tag="VGA15_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX9591697.1"
                     /translation="MTLQELRKMLEPALRRIFHLYWRFARGMTLGVRAVVLDGEDRVF
                     LVKHSYVSGWHLPGGGVEVGETFGDALRRELMEEGRIEMLGEPVLHGVFLNSHVSRRD
                     HVAVYLVRHFSQDRPPEPNREIVACGFFEPGALPAETTKGTRLRISEVLENRRPISTW
                     R"
     gene            1044..1931
                     /locus_tag="VGA15_28645"
     CDS             1044..1931
                     /locus_tag="VGA15_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX9591698.1"
                     /translation="MAAFTLAHLSDPHLPPLPSLRLGDLAGKRALGYLNWKRNRRKVH
                     KRDVVDALVADMQAQRPDHIAVTGDLVNLALEAEFAPARAWLESVGASDRVTVIPGNH
                     EAYVRATQHRFAEVWGHYLDGDDATGGVTFPSLRRRGPLALISVSSAVPTPPLMATGW
                     LGRTQLDALRRILTGLSTAQAFRVLLIHHPLRSDSHIKRLTDSSELLALLKVHGVELI
                     LHGHDHVHSTIWIDGPKGTIPVVGVPSASAVAHGRYPAAAYNLFSIERDGAAWRCEQT
                     VRGIDDTRRVQQLRQTRLI"
     gene            complement(2142..2276)
                     /locus_tag="VGA15_28650"
     CDS             complement(2142..2276)
                     /locus_tag="VGA15_28650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591699.1"
                     /translation="MTWVLHTPQAAVLGADNYFPQKFELANKQVIKTGYHIAGSGQAN
                     "
     gene            2275..>2493
                     /locus_tag="VGA15_28655"
     CDS             2275..>2493
                     /locus_tag="VGA15_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591700.1"
                     /translation="MGEIRKLKSSRGGDPPRESTPRRLAAIVAGDIAGYSRLMQIDEE
                     GTHLRVKRIERDLIEPSIVGHHGRLVKTT"
BASE COUNT          476 a          784 c          747 g          486 t
ORIGIN      
        1 cggcatcgat atcggcgaac atccgccgca ccacatggcc cttgccctga cgtcgttcgc
       61 tgcgcacctc cgccccggcc tcgcgcgcta ccgccatggt gcgatccgac gaattgttgt
      121 catagacaaa gatttcggct gttggcaggg ctttgcgaaa atcggtgacc acggtagcga
      181 ccgcggcttc ctcgttgaag cacggcacca gaacggccac ccgcggctgc gctgtcctgg
      241 cttgtgggaa ctcggtttgt gcaaattgcg ccagcgcctc ggccatgccg gccagatcgc
      301 cgcgaagttt tgccgggcgt cctgggttcg gcaccgcgct catcagccca atccttaacg
      361 attattaggg ttttgacccc cgatcatagg gaacgttcgc taacgttctc taacgccagg
      421 tggagattgg tcgcctgttt tcgagcactt ccgatatccg cagccgggtg cccttggtcg
      481 tctccgccgg cagcgctccc ggctcgaaga aaccgcaagc gacaatctcg cggtttggct
      541 cgggcggccg atcctggctg aagtgacgga ccagatagac cgcgacgtgg tctcggcggg
      601 aaacgtggct gttgaggaac acaccgtgca ggaccggctc gcccagcatt tcaattcttc
      661 cctcttccat cagctcgcgt cgcagcgcat cgccaaatgt ttcaccgacc tcgacgccgc
      721 cgcccggcag gtgccagccg gaaacatagc tgtgcttgac caggaacaca cggtcctcgc
      781 cgtcaagcac cacggcgcga accccgagcg tcatgccgcg ggcgaaccgc caatagagat
      841 ggaaaatccg ccgcagtgcc ggctctaaca tctttcgcaa ctcttgcagg gtcatcgcca
      901 ccgaaattcc ccaaatccag acatcgcgtt tgatccttgc gttgcggcgg ccagcttgcc
      961 aatacattgt cggatttgac agggtatcac gagatgatcg tgcagcggcg ccatcacacg
     1021 ggcaacagca gcggatgcgg ctgatggcgg catttacgct ggcccatctg tccgatccgc
     1081 atcttccgcc gctgccaagc ctgcggcttg gcgacctcgc cggcaagcgc gcgctcggtt
     1141 atctcaactg gaaacgcaac cgccgaaaag tccacaagcg cgacgtggtc gatgcgctgg
     1201 tagcggacat gcaggcgcaa cggccggatc acatcgcggt caccggcgac ctcgtcaacc
     1261 tcgcgctcga ggcggagttt gctccggcgc gggcatggct ggagagcgtc ggcgcatccg
     1321 atcgcgtcac ggtgatcccc ggcaatcacg aagcctatgt ccgcgcgacg caacaccgct
     1381 ttgcggaagt ttggggacat tatctcgatg gcgacgacgc gaccggcggc gtcacctttc
     1441 cgtcgctgcg ccggcgcggc ccgttggcgc tgatcagcgt atcctcggcg gtcccgaccc
     1501 cgccgctgat ggcgacggga tggctcgggc gcacccagct tgatgcgctc cggcgcattc
     1561 tgacaggtct gtccacggcg caggcttttc gtgtcctgct gattcatcac ccgctgcgct
     1621 cggattcgca catcaagcgg ctgaccgatt catccgaact gctcgccctc ctgaaggttc
     1681 acggcgtgga gttgatcctg cacgggcatg atcacgttca ttccacgata tggatcgacg
     1741 gaccgaaggg caccattccc gtggtcggcg tgccttcggc ctccgcggtc gcgcatggcc
     1801 gctatcctgc cgcggcctac aatctgtttt cgatcgagcg cgatggcgcg gcgtggcggt
     1861 gcgagcagac cgtacggggc atcgacgata cgcggcgcgt ccagcaattg cggcagacgc
     1921 ggctgatcta gaacgccgga actcacaaag aagaaggccg atgaaaatac caacctgccg
     1981 cccgacgtct atcaccgagt atgagcggcg tggaggtttt ttgaaccgaa gcggacatcg
     2041 ggcgagcgat ccgggaggcg ctgaatttgc gagatgtgaa gcagttcaca ttcgaatacc
     2101 gcggttcgcg ctatttggag tacatccgtt tgaggaaggc ttcaattggc ttgtcccgac
     2161 ccggcaatgt gataacctgt tttgataact tgtttattgg cgagttcaaa tttctgcgga
     2221 aagtagttgt ccgcccctaa caccgccgct tgcggtgtat ggagaaccca ggtcatgggt
     2281 gaaattcgca aattgaaatc gagcagaggc ggcgatcccc cgcgagagtc aacgccgcgc
     2341 cggcttgctg cgattgtcgc tggcgatatc gcgggctaca gccgcctcat gcagatcgat
     2401 gaagaaggaa cgcatcttcg cgtaaagcgg atcgagcgtg acctcataga gcccagcatt
     2461 gtggggcatc acggaaggct cgtcaaaacc act
//
LOCUS       DASWGM010001066         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_275165,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001066 DASWGM010000000
VERSION     DASWGM010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275165"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..772)
                     /locus_tag="VGA15_28660"
     CDS             complement(167..772)
                     /locus_tag="VGA15_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEX9591701.1"
                     /translation="MTADTIARLKVTLDHVKPTVLRRVEVPFDIRLDRLHLTLQAAIG
                     WTNSHLYEIRARDVRWSTPYPDADWGNDFLDARKACLGDVLEDVGTKTLRYLYDFGDG
                     WEHTIKIERLTDPEFGVLYPRLIEASGRCPPEDCGGPWGYAELLEAIADPKHERHDEL
                     SVWIGDEFNPDAVEADWLTEEVAALAKRWSRKPATQRRRTA"
     gene            complement(769..2385)
                     /locus_tag="VGA15_28665"
     CDS             complement(769..2385)
                     /locus_tag="VGA15_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX9591702.1"
                     /translation="MGAGDSLPDDVEALKRLVRARDAELAQAHAEASSAEALIAHLRL
                     TIEKMRRELFGPRSERKARLLDQMELELEELEAAASEDELASEKAAAVAETTPVRAFT
                     RKRPARKPFPAHLLRERVIVPGPTACACCGSTRLAKLGEDVTETLEVVPRQWKVVQYV
                     REKFTCRACEKISQAPAPFHVLPRGFAGPSLLAMVLFEKYGQHQPLNRQSERYAREGI
                     DLSVSTLADQVGGCAAILHPIYDLICAHVFAGSRVHGDDTPVPVLAKHQTRKGRLWTY
                     VRDDRPFGGHDPPAAVFFYSRDRAGEHPGRHLAGYAGILQADAYAGFQRLYEAGRKPE
                     PITEAACWAHSRRKFFVLADVTAKARGKLPVLSPLALEAVKRIDAVFDVEREISGLSA
                     VKRLAARQSRVAPLVAELEAWMRAERIKLSRHSEVAKAMDYILKRWKAFTRFLADGRI
                     CLTNNAAERELRGIALGRKSWLFAGSDRGGERAAVMYTLIQTARMNGIDPQAWLADVL
                     ARINDHAIHRLDELLPWNWAVEMERRKLAA"
     gene            complement(2434..2796)
                     /gene="tnpB"
                     /locus_tag="VGA15_28670"
     CDS             complement(2434..2796)
                     /gene="tnpB"
                     /locus_tag="VGA15_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX9591703.1"
                     /translation="MIPVPTRVQVWLATGHTDMRKGFDGLALLVQEMLRRNPHNGHLF
                     VFRGRRGSLIKVLWHDGQGMCLFAKRLERGRFIWPSTVARDGTDGTVTITPAQLGYLL
                     EGIDWRLPQHTWRPQAAG"
     gene            complement(2793..3170)
                     /locus_tag="VGA15_28675"
     CDS             complement(2793..3170)
                     /locus_tag="VGA15_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX9591704.1"
                     /translation="MTSSQVEVITSVQRRRRWTAAEKERLVAASLEPGAVASEIARDA
                     GIHPSQLFGWRRQLCQRRDGIPAFAAVTLATGGPAAASTPTPPGMIEIEFAGGPRMRI
                     SGTVDPATLSAVVVALGPRARRR"
     gene            3233..3574
                     /locus_tag="VGA15_28680"
                     /pseudo
     CDS             3233..3574
                     /locus_tag="VGA15_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
BASE COUNT          683 a         1256 c         1225 g          718 t
ORIGIN      
        1 tggtgcccac gtcttccagt acgtcgccaa ggcatgcctt gcgcgcgtcg aggaagtcgt
       61 taccccaatc ggcgtcggga tagggcgtgc tccatcggac atcgcgtgcg cgtatctcgt
      121 agagatggct gttggtccgt aaccgtccgg tgaatgccgc ggctcgctag gcggtgcggc
      181 ggcgttgggt ggcaggtttg cgcgaccagc gtttggcgag tgcggcgact tcttcggtga
      241 gccaatctgc ttcgacggcg tcgggattga attcgtctcc gatccacacg ctgagttcgt
      301 cgtggcgttc gtgtttggga tcggcgatcg cttcgagcag ctcggcgtag ccccaggggc
      361 cgccgcagtc ttcgggaggg cagcgtccgc tggcctcgat caggcggggg tagagtacgc
      421 cgaactcggg atcggtgagc cgctcgatct tgatcgtgtg ttcccagccg tcgccgaagt
      481 cgtagagata tctcagcgtc ttggtgccca cgtcttccag tacgtcgcca aggcatgcct
      541 tgcgcgcgtc gaggaagtcg ttaccccaat cggcgtcggg atagggcgtg ctccatcgga
      601 catcgcgtgc gcgtatctcg tagagatggc tgttggtcca gccgatcgcc gcctgcagtg
      661 tcagatgcag acgatcgagc cgaatatcga acggcacctc aacgcggcgc agaaccgtgg
      721 gtttgacgtg atcgagcgtg accttgaggc gagcgatggt atcggcggtc atgccgccag
      781 tttacgccgc tccatctcga ccgcccagtt ccacggcaga agctcgtcga gcctgtggat
      841 tgcgtgatcg ttgatacggg cgagcacgtc ggcgagccag gcttgcggat caataccgtt
      901 cattcgtgcc gtctggatca acgtgtacat cacggcggcg cgctcgccgc cgcggtcgga
      961 gccggcgaac agccatgact tgcgaccgag ggcaatgccg cgcagttcgc gctcagcggc
     1021 gttgttggtg aggcagatgc ggccgtcggc gaggaatcgc gtgaacgcct tccagcgctt
     1081 gagtatgtaa tccatggcct tggcgacttc ggaatggcgg gagagtttta tgcgttcggc
     1141 tcgcatccaa gcttccaatt cggccaccaa aggcgcaacg cggctctgcc gagcggcaag
     1201 gcgtttgacg gcggacagcc cgctgatctc gcgctcgaca tcgaagaccg catcgattcg
     1261 ctttaccgct tcgagcgcca gcggcgagag cacgggcagc ttgccgcgcg ccttggcggt
     1321 gacatcggcg agcacgaaga acttgcgccg gctgtgcgcc cagcaggcgg cttcggtgat
     1381 gggctccggt ttgcggccgg cctcgtagag ccgctggaag ccggcatagg catcggcctg
     1441 caggattccc gcatagccgg cgagatggcg cccgggatgc tcacccgcgc gatcacgtga
     1501 atagaagaac acggccgccg gcggatcatg gcccccgaag ggacgatcgt cgcggacgta
     1561 ggtccacaat cggcccttgc gcgtctgatg cttggccagc accggcacgg gagtgtcgtc
     1621 gccgtgcacg cgactgccgg caaaaacgtg ggcgcagatg agatcgtaga tcggatgcag
     1681 gatggcagcg cagccgccga cctgatcggc cagcgtcgaa acgctcagat cgatgccttc
     1741 gcgggcatag cgctcggact ggcgattgag cggctgatgt tgcccgtact tctcgaacag
     1801 gaccatggcg agcaggctcg gcccggcgaa gccgcgcggc agcacatgga acggcgcggg
     1861 cgcctgcgag atcttctcac acgcccggca ggtgaacttc tcgcgcacgt attgcacgac
     1921 cttccattgc cgcggcacca cctccagcgt ctcggtgacg tcctcaccga gcttggcaag
     1981 tctggtcgag ccgcagcagg cgcaggccgt cgggcccggc acgatgaccc gctcgcgcag
     2041 cagatgcgct gggaatggct tgcgcgcggg ccgcttacgg gtgaacgcac gaaccggcgt
     2101 cgtctctgcg accgccgccg ccttctcgct ggccagttca tcctcgctgg ccgcagcctc
     2161 gagctcttcg agctcaagct ccatctggtc gagcagccgc gccttgcgct cgctgcgcgg
     2221 tccaaaaagc tcacgccgca tcttctcgat cgtgagccgc aggtgcgcga tcaaagcttc
     2281 cgcgctcgac gcctccgcat gcgcctgcgc aagctcggca tcgcgggcgc gaaccaagcg
     2341 cttcaatgcc tcaacatcat caggaagaga atcaccagca cccatcgcta aagaaggaat
     2401 cacaagatgc tgctcttgtg aatcgcgcgc gcttcaaccc gcagcctgtg gccgccacgt
     2461 gtgctgcggc aggcgccaat cgatcccttc cagcaaatat cccagctgcg ctggcgtgat
     2521 cgtcaccgtg ccatccgtgc cgtcgcgcgc aaccgtcgat ggccagatga aacggccccg
     2581 ttccagccgc ttcgcaaaca ggcacatgcc ctggccgtcg tgccacagca ccttgatcag
     2641 gctgccgcgc cggccgcgga acacgaacag atgtccattg tgcggattgc gcctgagcat
     2701 ctcctgcacc agcagcgcca ggccatcaaa acccttgcgc atgtcggtgt ggcccgtcgc
     2761 cagccacacc tgcactctgg tcggcactgg aatcatcgcc gtcgtgcccg cggccccaac
     2821 gccacaacca cggccgatag cgttgccgga tcgaccgtcc cgctgatccg cattcggggt
     2881 ccgcccgcaa actcgatctc gatcattccc ggcggcgtcg gcgtggatgc tgctgcaggc
     2941 cctccggttg ccagcgtgac cgcagcaaat gccgggatgc catctcgccg ttggcacaac
     3001 tgtcgtcgcc agccgaacag ttggctcgga tgaatcccgg catcgcgggc aatctccgat
     3061 gccaccgcgc caggctccag tgacgcggcc acaagccgct ccttctccgc cgccgtccag
     3121 cgacgccgac gctgaacaga ggtgatgacc tcgacctggg aactcgtcat gtgagtattc
     3181 ctatgaatac tcacatgact cacatcacag accgggccgc aaggcggtcc tcaccggaag
     3241 agtaccgcaa gcctggagcg gcaccgccac ggttggccgc aaggaagaat ggccgtcgtg
     3301 gacaccgacc gccggcgaac atgcgcggct tgggcccctc ccggccttcg tgagtggcgg
     3361 gccgcaaaat ccgatgggtg cgcgcgggct atatctgtac tctggtggcc tggacacgca
     3421 gtaccgtatc cacggcacca accagcccga atatatcggc cgagccatct cctcgggctg
     3481 catccgcatg accaacgagg acgtgatcga tctctacagt cgcgtcaaga tcggcacgat
     3541 tgtcgttgtg ctcgcgccaa ctcactggac atagggacac cacgtggctc ccaacgccta
     3601 accggtcgtt ctagtcacat gatggtttgc ttaaaggtgc ttgaaatgaa cccgcgcgcg
     3661 gtgtcgtgtt aataggcgga tttagcatct taggtgtctt gcttggagca gcccttgcct
     3721 gccttgccga tcgatatccg cgacacaggc caatgatgga attagtggct gggtggcttt
     3781 tcattgcggg ttttggtttg ctgggcgccg acctcgcgag cgttttcgga ctgccgatgc
     3841 gctaaaatcc gtgcgctcgc ctaaccacgc taagaaggcg ct
//
LOCUS       DASWGM010001067         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1516885,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001067 DASWGM010000000
VERSION     DASWGM010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516885"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VGA15_28685"
     CDS             <1..1180
                     /locus_tag="VGA15_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX9591705.1"
                     /translation="VPRKGNLVARLRGTGARKPILLVAHLDVVPASREDWSVEPFKLT
                     EQDGYFYARGSGDDKYMAAAFVTNLIRYKQEGYKPDRDIILALETDEEIFDRDALGIQ
                     WLLKHHRDLIDAEFALNEGGGVGLKDGKPIRNSVQTSEKVVLNYQLDVKNKGGHSAVP
                     LKDNAIYHLAEGLVRLSKFSFPLKLNETTRAYFERTAQFEGEQTAADIRALLSDKPDP
                     SAMSFVRLAANPVYNSQLRTTCVATMLEGGQAINALPQLASAKVNCRVMPGEPVDEVK
                     ATLERILADDQIVVTQLGSPVLSAPSALNEEVMGSIEKLSQQFWPGAVVLPVMSSGAT
                     DGSFLRNAGIPTYGHSGLASDISDGGRIHGKDERVLVKSFYQGEDYLYRLVKMLSGGG
                     "
     gene            1237..3591
                     /locus_tag="VGA15_28690"
     CDS             1237..3591
                     /locus_tag="VGA15_28690"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX9591706.1"
                     /translation="MTANSITLNPADHLPDLESPAAVPGLLNAVDVRRDSWGIPHIRA
                     ASQLDAFAGLGFVHAQDRLWQMEALLRRGTGRYAEWLGKSALAGDILARKVDTVGASR
                     RDFAILKSETRSMLEAYARGVNAFIALRRWPAEYAILKTEPAPWEPWHSIAVMRQIGF
                     LMGSVWWKLWRAAAVPIVGVAEISKLRFDDGGDDLLCIPPGEEGARYLASLGDLKPGL
                     EALIGHGNDQQEAELAGGSNNWALSPDRTATGRPLLAGDPHRVLEMPNMYAQTHLACD
                     EFDIVGLTVPGVPGFPHYGHTANVAWCVTHAFVDIHDLYIERFDAGAENTRFKGGWTP
                     VKRRKESVSVRGEKDVEIEVIETCHGPVIAGDPAAGTAVALRSAQFAVPDKSFDCMLP
                     MLRAASVEQLYEATRDWGLMDHNLVAGDTAGHIGNRVRARVPARPKSNGWLPVPGWTG
                     EHEWNGMVPFEEMPCCIDPAGGTIVTANNRVMENGKRYLSTDSMPPHRARRIWQRLAQ
                     LSKAGVDDMASVHRDLVSVVALEFRDRIRNVKVLGHAAELRDRIINWNGEMTADSHSA
                     VAYVALRTALTKLVAEKSGLSAVSESPYAKVTPGIFGESQIWWTVPQLLRANDTGLFR
                     PATWNELLAQALERAALNFPPESFSSGTFQSETWAELHRPTLRHPLSSAFPEFASVLD
                     RPCVPVGGDNDTVFATGYVARLGMRATYGSLCRYVFDVGAWDNCKWIVFHGSSGHTES
                     PWYDNQNATWAAGEMVPMLYDWTKIEQLATTHQKLMPVEAPRAA"
BASE COUNT          730 a         1114 c         1226 g          731 t
ORIGIN      
        1 ggtgccgcgc aagggcaatc tggtcgcgcg cctgcgcggc accggcgcgc gcaagccgat
       61 cctgctggtg gcgcatctcg acgtcgtgcc ggccagccgc gaggactggt cggtcgagcc
      121 gttcaagctg accgagcagg atggctattt ctacgcgcgc ggcagcggcg acgacaaata
      181 catggcggcg gcctttgtca ccaacctcat tcgctacaag caggaaggct acaagcccga
      241 tcgcgacatc atcctggcgc tcgagaccga cgaggaaatc tttgaccgtg acgctctggg
      301 gatccagtgg ctgctcaagc accatcgcga cctgatcgat gctgaattcg cattgaacga
      361 gggcggcggc gtcggactga aagacggcaa gccgatccgg aacagcgtcc agaccagcga
      421 gaaggtcgtg ctgaattatc agctcgacgt gaaaaacaaa ggtggccaca gcgcggtccc
      481 attgaaagac aatgcgatct atcatctggc ggagggactg gtgcgtctct ccaaattcag
      541 ctttccgctg aagctcaacg agaccacccg cgcttatttc gagcgcacgg cgcagttcga
      601 gggtgagcag accgcggccg atatacgcgc gctgctgtcc gataaaccgg atccgtccgc
      661 gatgtcgttt gtccggctcg cagctaatcc ggtctataat tcccagctgc gcaccacctg
      721 cgtggcgacg atgctggagg gcgggcaggc gatcaacgcg ttgccgcagc tggcgagcgc
      781 caaggtcaat tgccgggtca tgcccggcga acccgtcgat gaggtcaagg cgaccctcga
      841 gcgcatactc gccgacgatc agatcgtggt cacccaactc ggttcgccgg tcctgagtgc
      901 gccgtcggca ctgaatgagg aggtcatggg atcgatcgag aaattatcgc agcagttctg
      961 gcccggcgcg gtcgtgctcc cggtcatgag ctcaggcgcc accgacggca gcttcctgcg
     1021 caacgccggc attccgacct atggccactc cgggctcgcc agcgatatca gcgacggcgg
     1081 ccggatccac ggcaaggacg aacgggtgct ggtgaaatcg ttctaccagg gcgaggacta
     1141 tctctatcgg ctggtcaaga tgctgtccgg cggcggttag caaagaagaa ttccacttcg
     1201 ccatcgcagc cgagcaggga cacatcagaa ggccccatga cagcaaactc gatcacttta
     1261 aatcccgcgg accaccttcc cgatctggaa agcccggcgg cagtgccggg tttgttgaac
     1321 gcggtcgatg tgcggcggga cagttgggga attcctcata ttcgcgcggc ttcgcagctc
     1381 gatgcctttg ccgggcttgg gttcgttcat gcgcaggacc ggctatggca gatggaagcg
     1441 ctgttgcggc gtggcaccgg gcgttacgcg gaatggctcg ggaaatcggc gctggccggc
     1501 gatattctgg cgcgaaaggt cgacaccgtt ggggcatcgc ggcgcgactt cgccatcttg
     1561 aagtccgaga cgcgatcgat gcttgaggct tacgcgcgcg gggtaaatgc ctttattgcg
     1621 ctgcgccgat ggccggcaga atacgccatt ctcaaaaccg agcccgcgcc gtgggagccc
     1681 tggcattcga tcgcggtgat gcgtcagatc gggtttctga tgggctcggt gtggtggaag
     1741 ctctggcgcg ccgccgcagt cccgattgtc ggcgtcgccg agatctcaaa gctgcgtttt
     1801 gatgacgggg gtgacgacct gctttgcatt ccaccgggcg aagaaggtgc ccgttatctt
     1861 gcctcgctcg gcgatctgaa accgggactc gaggcattga tcggtcacgg caacgatcag
     1921 caggaggcgg aactcgccgg cggcagcaat aactgggctc tctcccccga ccgcaccgca
     1981 accggccgtc cgttgctcgc gggagaccca caccgtgttc tggaaatgcc caacatgtat
     2041 gcgcagacgc atcttgcatg tgatgagttc gacattgtcg gtctgacggt gccgggcgtg
     2101 ccgggatttc cgcactacgg tcacacggcc aacgtcgcct ggtgcgtgac gcatgcgttc
     2161 gtcgacattc acgacctcta tatcgagcgc ttcgacgctg gcgcagagaa tacgcgcttc
     2221 aaaggtggct ggacgccggt caagcgccgc aaggagagcg tttcggtgcg cggcgaaaag
     2281 gatgtcgaga tcgaagtcat cgagacctgt catgggccgg tgatcgcggg cgatccggcc
     2341 gccggcacgg cggtggcgct gcgttcagcg cagtttgccg ttccggataa atccttcgat
     2401 tgcatgctgc cgatgttgcg ggccgcgagt gtcgagcagc tctacgaggc gacccgcgac
     2461 tggggtttga tggaccacaa cctcgtcgcg ggcgatacgg caggacatat cggcaatcgc
     2521 gtgcgcgcca gggtgccggc acgtccgaag tcgaatggct ggctgccggt gccgggctgg
     2581 accggcgagc acgaatggaa tggcatggtt ccgtttgaag aaatgccatg ctgtatcgat
     2641 cccgcgggcg gaacgatcgt gacggccaac aatcgtgtga tggaaaatgg caagcgttac
     2701 ctgtcgaccg acagcatgcc gccgcatcgc gcccgccgga tctggcagcg gcttgcgcag
     2761 ctttcaaagg ccggagtgga tgacatggcc tctgtccatc gcgatctcgt cagcgtggtg
     2821 gcgctcgaat ttcgcgatcg tatccgcaac gtcaaggttt tgggccacgc cgccgaactg
     2881 cgcgaccgca tcatcaactg gaacggcgaa atgaccgccg attcgcatag cgcggtggcc
     2941 tatgtcgcgc tacggaccgc cctgaccaaa ctggttgccg agaaaagcgg cctgagtgcg
     3001 gtctccgaga gcccttatgc gaaagtcacg cccgggattt ttggcgagag ccagatctgg
     3061 tggaccgtgc ctcaactgct ccgcgccaac gacaccggac tgttcaggcc ggcgacctgg
     3121 aatgagctct tggcgcaagc gctcgagcgc gccgcgctga attttccgcc cgagagtttt
     3181 tcatctggga cttttcaatc agaaacttgg gccgagttgc atcggcccac cttgcgtcat
     3241 ccgctgtcat cagcgtttcc ggaattcgca agcgtgctcg acaggccctg cgttcccgtc
     3301 ggcggtgaca acgatacggt gtttgcaacc ggctacgtcg caaggctcgg tatgcgcgca
     3361 acttatggat cgctgtgccg ctacgtgttc gacgtcggag cgtgggacaa ttgcaagtgg
     3421 attgtttttc atggatcgtc gggtcacacc gagagtccgt ggtacgacaa ccagaatgca
     3481 acctgggcag ccggcgagat ggtgccgatg ctttacgatt ggacgaaaat cgagcagctg
     3541 gccacgacac atcagaagct aatgcccgtt gaggctccgc gcgctgccta aaatcgcgtc
     3601 gatcgaatga gtcatcagca ggagcgttat gcgtggcgct cgttgccgag gcgtggattg
     3661 agcggttgtg tctgttgccg catgagatat cctttttgaa acttggccgg agaagttggc
     3721 tttgactgaa agcaggattg cgggtctgga ccaaagcctg cgtcgacgcg ctcttcaagt
     3781 cgtgcccggc ggcatgtacg g
//
LOCUS       DASWGM010001068         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_275577,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001068 DASWGM010000000
VERSION     DASWGM010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275577"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VGA15_28695"
     CDS             complement(<1..166)
                     /locus_tag="VGA15_28695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX9591707.1"
                     /translation="MTTSPLILLAAGGTGGHLFPAEALGVELIRRGYRVRLATDSRAL
                     RYGGLFSKDTI"
     gene            complement(357..1508)
                     /gene="ftsW"
                     /locus_tag="VGA15_28700"
     CDS             complement(357..1508)
                     /gene="ftsW"
                     /locus_tag="VGA15_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX9591708.1"
                     /translation="MLSREQRNPLSEWWWTVDRLQLAAVIALMLGGLILSLAASPPVA
                     TRIGLDPFHFFNRHVMFLLPSFIVLICVSFLSPRQIRRSALIVFAISIALIVATLLVG
                     PEVKGSRRWITLVGLNIQASESAKPAFVVLAAWLFAESARRPEMPATSMALGLLMTLV
                     SLLVMEPDFGQTMLILMVWGALFFVAGMRMIWVLGLAGAASAGLFSAYLLFPHVAGRI
                     KRFMNPGTGDTFQVDTAMEAFSNGGWFGLGPGEGIAKRSLPDSHTDFVFAVAAEEFGI
                     ILCLALLALFAFVVIRALSRAYANEDMFSRFAALGLAILFGVQAAINMSVNLQLIPAK
                     GMTLPFISYGGSSIISLAYGVGMMLALTRHRPRTEMESIGNADAARGYA"
     gene            complement(1786..3186)
                     /gene="murD"
                     /locus_tag="VGA15_28705"
     CDS             complement(1786..3186)
                     /gene="murD"
                     /locus_tag="VGA15_28705"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX9591709.1"
                     /translation="MIPITSFAGKTVAVFGLGGSGLASCHALKAGGAEVVASDDGDDR
                     LVEAAQAGFITADLRKVSWANFAALVLTPGAPLTHPAPHWTVLAAQQAGIEVIGDIEL
                     FCRERRRHVPDAPFVAITGTNGKSTTTALIAHLMRVAGYDTQMGGNIGTAILSLEPPR
                     MGRVHVIEMSSYQIDLSPSLDPSVGILLNVSEDHIDRHGTLEHYAAVKECLVAGVQKQ
                     GTAIVGVDDGWSRNTADRIERAGQRVVRISVKNPLADGIYVEHETIVRASGGARSEIA
                     RIGGIGSLRGRHNAQNAACASACALALGVSTEVLQNGLRSFPGLAHRMEQVGRCGNVL
                     FVNDSKGTNADAAAHALSSFADIFWIAGGKPKLGGITGLTEYFPRIRKAYLIGEAAKE
                     FAATLEGKVAYEITETLDIAVANSARDAEASGLADPVVLLSPACASFDQYRNFEIRGA
                     KFRDLVTALPGVKPVV"
     gene            complement(3191..4297)
                     /gene="mraY"
                     /locus_tag="VGA15_28710"
     CDS             complement(3191..4297)
                     /gene="mraY"
                     /locus_tag="VGA15_28710"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX9591710.1"
                     /translation="MFYWLIELSNTVPFMGAFRTFLNVFRYITFRTGGAMVTGALFVF
                     LFGPWIIDHLRIRQGKGQPIRTDGPQSHLLTKIGTPTMGGLMILSGLVVSTLLWANPL
                     NPYVLIVLAVTLGFGFVGFYDDYLKVTKQTHSGFAGRIRLAIEALIALAACYALTRLG
                     RDPFSTSLVIPFFKDVVLNFGWFFVIFGAFIIVGAGNAVNLTDGLDGLAIVPVMIAAA
                     SFGMIAYLVGNAVFSDYLQINYVAGTGELAVLCGAVLGAGLGFLWFNAPPASIFMGDT
                     GSLALGGMLGTVAVAVKHEIVLAVIGGLFVLEAVSVIVQVVSFKLTGKRIFRMAPIHH
                     HFEQLGWTEPQIVIRFWIISVMLALAGLSTLKLR"
BASE COUNT          899 a         1385 c         1367 g          699 t
ORIGIN      
        1 cgatggtgtc cttggagaac aagccgccgt agcgcagcgc gcgggaatcg gtggcgaggc
       61 gtacgcgata gccgcgcctg atcagttcga cgccgagcgc ctcggccgga aacaaatggc
      121 cgccggtgcc gcccgcggcc aacaaaatca gaggcgaagt ggtcatgggc aattcttttt
      181 cagggtaccg tcatcgcgaa ccacggaacc gctggatgac gggctccgcg aagcaatccg
      241 gaggctgcag cacaaagcgg agctggattg cttcgtcacg gagcctgtca tcgggcgcgc
      301 attcgcgcaa cccgttggtt cctcgcaatg acgaaaagcg cgtcttcgcc gtgaggtcaa
      361 gcgtaaccgc gcgccgcgtc ggcgttgccg atcgattcca tctcggtacg cggtcgatgt
      421 cgcgtcagtg ccagcatcat gccgacgcca taggccagcg agatgatcga tgagccgcca
      481 taggagatga acggcaatgt catgcccttg gccggtatca gttgcagatt gaccgacata
      541 ttgatcgcgg cctgcacgcc gaacaagatc gccagtccca acgcggcgaa ccgcgagaac
      601 atatcctcgt tggcgtaagc gcgcgacaat gcgcggatga cgacgaaggc aaacagtgcg
      661 agcagcgcga ggcaaaggat gatgccgaat tcctccgccg ccaccgcgaa cacgaaatca
      721 gtgtggctat ccggcaggct gcgcttggcg atgccttcgc ccggtccaag cccgaaccag
      781 ccgccgttgg aaaatgcttc catcgcggta tcgacctgaa aagtatctcc cgtcccgggg
      841 ttcatgaagc gcttgatgcg gcctgcaacg tgcgggaaca aaagataagc actgaatagt
      901 ccggcgctcg cggcacccgc aagccccagc acccagatca tccgcatgcc cgcgacgaag
      961 aacaaggcgc cccacaccat caggatcagc atggtctggc cgaagtcagg ttccatcacc
     1021 aagagcgaca ccagcgtcat caggagtccg agcgccatcg aggtcgcggg catttccggc
     1081 cgccgcgccg attccgcgaa cagccacgcc gccagcacca cgaaggcggg ttttgcggat
     1141 tcggaagcct ggatgttgag gccgaccagg gtgatccagc gccgcgagcc ttttacctct
     1201 gggccgacga gaagcgtcgc cacgatcagc gcgattgaga tcgcgaatac gatcagcgcg
     1261 gagcgcctga tctgccgcgg cgacaggaac gacacgcaga tcagcacgat aaaggacggc
     1321 agcagaaaca tgacgtggcg gttgaagaag tgaaacggat cgagcccgat ccgtgtcgcc
     1381 accggcggac tggccgccag cgacagaata aggccgccca gcatcagcgc gataaccgcc
     1441 gcaagctgca ggcggtccac cgtccaccac cattcgctta aggggttgcg ttgttcacga
     1501 gagagcatgg gtggtcccaa tggcagaggt tggcccattg gtcgcccatg atggtttcta
     1561 aggggttaat aaattggaga tgtttcgaac gagttgggcg tcagcctcgt gccccggatg
     1621 ctgcgcagcg cactcgggtc cgcgcgacgc gcggcccgag cataggctcc gcgatgcgct
     1681 gctgatccgg ggcccatgac acgtgggtcc cggctctgcg gagcagcgtg aagaacgctg
     1741 caccgcgtcc gggacacaat tgcagctgca gcgctcaacg aaacctcaca ccaccggctt
     1801 gacgccgggc agcgcggtga cgaggtcgcg gaacttggcg ccacggattt caaaattgcg
     1861 gtactggtcg aacgacgcgc aggccggcga cagcaatacg accggatcgg caagccccga
     1921 tgcttccgca tcgcgcgcgg aattggccac cgcaatatcg agcgtttctg tgatctcgta
     1981 ggccaccttg ccctcgagcg tcgcggcaaa ctccttggcg gcctccccga tcagataggc
     2041 tttccggatg cgcggaaaat attcagttag tccggtgatg ccgccgagct tcggcttgcc
     2101 gccggcgatc cagaagatat cggcgaacga cgacagcgca tgcgccgccg catcggcatt
     2161 ggtgcccttg gaatcgttga cgaacagcac gttgccgcag cggccgacct gctccatgcg
     2221 gtgcgcgagg ccgggaaagc tgcgcaggcc gttttgcaaa acttcggtac tgacgccaag
     2281 cgccagcgcg cacgccgacg cgcacgcggc attctgcgca ttgtgccggc cgcgcaagga
     2341 accgatgccg ccgatccgcg cgatctcgct gcgcgcgccg ccggatgcgc gcacgatggt
     2401 ttcatgctcg acatagatac cgtcggccag cggatttttc accgagattc gaacgacgcg
     2461 ctggcctgct cgctcgatcc ggtcggcggt gttccggctc catccgtcgt cgacgccgac
     2521 gatcgcggtg ccctgctttt gcaccccggc gaccagacac tccttgacgg ccgcgtagtg
     2581 ttcgagcgtg ccgtggcgat cgatgtggtc ttcgctgacg ttgagcagga tgccgacgct
     2641 gggatcgagc gacggagata aatcgatttg gtacgacgac atctcgatga cgtgcacccg
     2701 ccccatgcgc ggcggctcca gtgacaggat cgcggtgccg atgttgccgc ccatctgggt
     2761 gtcgtagccg gcgacgcgca tcagatgcgc gatcagcgcc gtggtggttg atttgccatt
     2821 ggtgccggtg atggcgacga acggcgcatc cggtacatga cgccgccgct cccggcaaaa
     2881 cagttcgatg tcgccgatca cctcaatgcc ggcctgttgc gccgccagca cggtccagtg
     2941 cggcgccgga tgcgtcagcg gcgcgcccgg cgtcagcacc agagcggcga aattcgccca
     3001 cgacacttta cgcagatccg cggtgatgaa gcccgcttgc gcggcttcca ccagcctgtc
     3061 gtcgccgtcg tcactggcga ccacttccgc cccgcccgcc ttcaaggcgt ggcagctagc
     3121 aagcccggat ccgccgaggc cgaatactgc gaccgtcttg cccgcgaagg aggtgatcgg
     3181 gatcaaatga tcaccgcagc ttcagcgtgg agagaccggc cagcgccagc atcaccgaga
     3241 tgatccagaa ccggatcacg atctgcggct cggtccagcc cagttgctcg aaatggtggt
     3301 ggatcggcgc catccggaag atgcgcttgc cggtgagctt gaacgacacc acctgcacga
     3361 tcaccgacac cgcttccagc acgaacagac cgccgatcac ggccagcacg atctcgtgtt
     3421 tgaccgcgac cgcgaccgtg ccgagcatgc cgccgagcgc gagcgaaccg gtatcgccca
     3481 tgaagatcga cgccggtggc gcgttgaacc acaggaatcc caggcccgcg ccgagcaccg
     3541 cgccgcacag caccgcgagt tcgccggtgc cggcgacgta gttgatctgc agataatccg
     3601 agaacaccgc gttgccgacc aggtaggcga tcatgccgaa gcttgctgcc gcgatcatga
     3661 ccggcacgat ggccagaccg tcgagaccgt cggtcaggtt gaccgcattg ccggcgccga
     3721 caatgatgaa cgcgccgaag atcacgaaga accagccgaa attcaaaacc acatccttaa
     3781 agaacggaat caccagggag gtcgagaacg ggtcgcgtcc gagccgggtc agcgcgtagc
     3841 aggccgcgag cgcgatcagc gcttcgatcg ccaggcggat gcgcccggca aagccgctat
     3901 gggtctgttt tgtcactttc agataatcgt catagaagcc gacgaagccg aagcccaggg
     3961 ttaccgcaag cacgatcagg acatagggat tgagcggatt ggcccatagc agcgtcgaga
     4021 ctaccagccc ggaaagaatc atcagcccgc ccatcgtcgg cgtgccgatc ttggtgagga
     4081 gatgcgattg cgggccgtcg gtgcgaatcg gctggccctt gccctggcga atccgcaagt
     4141 gatcgatgat ccaggggccg aacaggaaca cgaacagcgc cccggtcacc atcgccccgc
     4201 cggtgcgaaa ggtgatgtag cgaaacacat tcaggaatgt gcggaacgcg cccatgaagg
     4261 gaaccgtgtt ggaaagctcg atcagccagt aaaacattca gaccgcccta taccgcggct
     4321 tcgccgtgcg cggtcttgcc gggaaagcgc
//
LOCUS       DASWGM010001069         5305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1517567,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001069 DASWGM010000000
VERSION     DASWGM010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517567"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1029
                     /locus_tag="VGA15_28715"
     CDS             313..1029
                     /locus_tag="VGA15_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX9591711.1"
                     /translation="MNSCHGVLYISALCVAAALAASPAHAQTRVGEAAVVKNEVVRVM
                     ASATSQINVGDGVLRDEIVRTGLDSAARLVMADSTNLSLGPSATVKLDRTVFNDEHTY
                     RDIAIRLTTGAFRFVTGHSEKAAYKITTSVATIGVRGTILDILSQRGKTTVVLQEGAS
                     HVCTIGGQCTELTEPGDTAVITSTAGRVTIQKTNNPPWTFAGTCGAAAGLCSTTQYAD
                     ATPTITSPVDDDPTGMLCGR"
     gene            1032..2090
                     /locus_tag="VGA15_28720"
     CDS             1032..2090
                     /locus_tag="VGA15_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591712.1"
                     /translation="MNKFARSFIPSAALIASIWLVLAPLHPSPAAAQECFDNCCSEGC
                     DSTPPPPPPVTGADSSAGSIGDLGKQRFNQMITHRVLGSVLLGVNEQVNCSDCVSAFG
                     SAGSFSAGIHGRKELTPNLSLLAGIAYTQYSEGGYHITSAPIGAFALRYDFVDWGSSR
                     PFFDVGAILSPSEKARYSRSYMTSLGPVSLESQTHSENYGAYGRAGWVSRISPRDEVA
                     ASVEVWQLWQRVKGYSDPTVAFNPFDATIATGTDKTNLVKVGGQWTHLFGNNVEGNIN
                     GGFVQSFANHSGIVASVTGDGTVTPTLGNQSWFEYGGRLGFRITKGWVADLFVNGTAG
                     PQPVGNTIHGGVGLRVNY"
     gene            complement(2084..4330)
                     /locus_tag="VGA15_28725"
     CDS             complement(2084..4330)
                     /locus_tag="VGA15_28725"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX9591713.1"
                     /translation="MSAPGRSEQTLAAAMAHHRAGRLTDAERLYRIVCDTDPNNARAF
                     HLLGLVAHQLRRPDAASYLGRAVTLDPDFAEAHNDRGVILAAGGLFADAISCFERAVT
                     LNPGYSEARTNLGRGLRSLGRLDEAVAQFERVLKSTPESPVAHFNLASAFELAGQHPN
                     AEKHYRRAISLRPDFVDAYIHLASLLQSTDRLPEALAHAERAVALRPDSAGVRNNLGN
                     ILRALGRRDEAIAQYQAALGIDPGSFMAHYNCGVALRGETRIAEARAHFARAFALKPD
                     FWQAELALCMAELPAIYQGASEIAERRAAYASRLARLCADAESAGAPAVLSEAIGSHQ
                     PFYLPYQGRNDRELQSLYGRLVCRMMAARYTTAALPDPPGPREPIRLGIVSGFFRQHS
                     NWKIPVKGWLKMLDRTRFHVSGYYTSADRDSETDAAATICDRFVQGPLSLDAWRRTIL
                     ADVPHVLIFPEIGMDKVSAQLAAQRLAAVQCASWGHPVTSGFPTIDYFISSDLMEPES
                     AADHYSEQLIRLPNLSIYYEPADVPPVAVDRAQLGLRTGAVVYWCCQSLPKYLPQFDE
                     VFARIAAEVPDCQFTFIEFGGGSGVTEMFRRRLRRAFKAVGLNAENHCVFLPRLAPDR
                     FVAAIGQCDVVLDSIGWSGCNSILESLVHNLPIVSFAGEMMRGRHTAAMLGMMDISET
                     TARTIEDYVSIAGDLGRNVTTRIELSARIAANKHRVYRDRDCIVALETFLDGAVRNAA
                     RGTDATAQ"
     gene            complement(4361..4663)
                     /locus_tag="VGA15_28730"
     CDS             complement(4361..4663)
                     /locus_tag="VGA15_28730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591714.1"
                     /translation="MLMRTIVTLAAILALGSSHGWANGHKAPRHHRHGYMVRYQEQPV
                     LPAPYGGGRLVLHPASNIACNTRDRATRALPCDQPVWVYGSPCEVDLGLGRYRSCD"
     gene            complement(4684..>5305)
                     /locus_tag="VGA15_28735"
     CDS             complement(4684..>5305)
                     /locus_tag="VGA15_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX9591715.1"
                     /translation="GLPAEGLVLCSFNNSYKISPVFFDIWMRLLRAVPGSVLWLLEAN
                     PLVRGNLRSEAEKRGVDSGRLIFAPVVPPAEHLARHRHADLFLDTLPCNAHTTASDAL
                     WAGLPVLTCCGDTFAGRVAGSLLMAIDMPELVTKSLEEYEQAALALARSPQRLIALRE
                     KLQSNRDASALFDLPKLTGNIEAAYARMWQTWLAGQTPAAFSIEGA"
BASE COUNT         1075 a         1656 c         1616 g          958 t
ORIGIN      
        1 gaccagaagt gcgaggtgtg caactacaaa gacaacagcg acaacaatga cgcttcaggc
       61 agtcgaattg tcgaaaacag acagtgatgt tcgcacgagg ctcacaaatc aatgacgttg
      121 caacgatcgc gtgtcattcg aatggatgag ggggcgattt ttaaatccat ttgcattgaa
      181 cgcatttcga tttcgcgcgc actaatcaaa gcggtagtta tgagtcgctt gtggcaaatt
      241 tacaatatcg cccacccggg cggtccggta cgatggcgga ataaattgat cggcattgaa
      301 ctcggaattg aaatgaattc gtgtcacggt gttctgtata tttccgcgtt atgtgtagca
      361 gcagcccttg cggcaagtcc cgctcacgcg cagacgcgcg tcggcgaagc ggctgtcgtc
      421 aagaacgaag tcgtccgcgt gatggcgtcg gcaaccagcc agatcaacgt cggcgacggc
      481 gtgctgcgcg acgagattgt gcgcaccggc ctcgacagcg cagcgcggct ggtgatggcc
      541 gacagcacca atctgtcgct cggtcccagc gcgacggtca aactcgaccg caccgttttc
      601 aacgacgagc acacctatcg cgatatcgcg atcaggttga cgaccggcgc gtttcgcttc
      661 gttaccggac actcggaaaa ggccgcctac aagatcacca cgtcggtcgc gacgatcggc
      721 gtgcgcggca ccatcctcga tatcttgtcg caacggggca agaccaccgt ggtgctgcag
      781 gaaggcgcat cgcatgtctg caccatcggc ggccagtgca ccgagctgac cgagcccggc
      841 gataccgccg tcatcacctc gaccgccggc agggtcacga ttcaaaagac caacaacccg
      901 ccctggacct ttgccgggac ctgcggagcg gcggcgggac tttgcagcac cacccagtat
      961 gcggatgcca cgccgacaat aacatctcct gtcgacgacg atcctactgg catgctgtgc
     1021 gggcgctgac gatgaacaaa ttcgcacgca gcttcatccc atcggccgca ctcatcgcat
     1081 cgatctggct tgtactggcg ccgctccatc cgtcgcccgc ggcggcgcag gaatgttttg
     1141 acaattgctg ctccgagggc tgcgacagca cgccgccgcc cccaccgccg gtaaccggcg
     1201 cggattccag cgccggatcg atcggcgacc tgggcaagca gcgcttcaac cagatgatca
     1261 cccaccgcgt gctgggctcg gtgctgctcg gcgtcaatga gcaggtcaat tgcagcgact
     1321 gcgtcagcgc gttcggttcg gcgggatcat tctcggccgg catccacggc cgcaaggaac
     1381 ttacacccaa tctgtcgctg ctggcgggta tcgcctacac ccaatacagc gaaggcggat
     1441 accacatcac cagcgcgccg atcggcgcgt tcgcgctccg ctacgatttc gtcgactggg
     1501 ggtcgtcacg ccccttcttc gacgtcggcg ccatcctgtc gccgtccgag aaggcgcgct
     1561 atagccgcag ctacatgacg agcctcggcc cggtttcgct tgagagccaa acccacagcg
     1621 agaactacgg cgcctacggc cgggccggct gggtcagccg catctcgccg cgcgacgagg
     1681 tcgccgcgtc cgtcgaggtg tggcagctct ggcagcgggt gaagggctac agcgacccga
     1741 ccgtcgcgtt caatccgttc gacgccacga tcgcaaccgg caccgacaag accaatctcg
     1801 tcaaggtcgg cggacagtgg acgcatctgt tcggcaacaa cgtcgagggc aacatcaatg
     1861 gcggcttcgt gcagtcattc gcgaaccatt cgggcatcgt agcctccgtg accggcgacg
     1921 gcaccgtgac accgacactc ggaaaccaga gctggttcga atatggcggc cggctcggct
     1981 tccgcatcac caagggctgg gtcgccgacc tcttcgtcaa cggcaccgcg ggaccgcagc
     2041 cggtcggcaa caccatccat ggcggcgtcg ggctgcgggt gaactactga gccgtagcgt
     2101 cggtgccgcg cgcggcattt cgaacggcgc cgtccaaaaa tgtttccagg gcgacgatgc
     2161 agtcgcgatc gcgatagacg cgatgcttgt tcgccgcaat cctggcggac aattcaatcc
     2221 gtgtcgttac attgcggccg agatcacccg caattgaaac atagtcttca attgtccgcg
     2281 ccgtcgtctc gctgatatcc atcatgccca gcatggcggc ggtatggcgc ccgcgcatca
     2341 tctctccggc gaacgataca atcgggaggt tgtgaaccag gctttccagg atcgagttgc
     2401 aacccgacca tccgatgctg tcgaggacga cgtcgcattg accgatggcc gcgacgaagc
     2461 ggtccggcgc aagtctcggc aggaagacgc aatggttttc cgcgttgaga ccgacggctt
     2521 tgaacgcgcg ccggagccgg cgtcgaaaca tctcggtgac gccgctgccg cctccgaact
     2581 cgataaaagt gaactgacag tccggaacct cggcggcaat gcgtgcgaaa acttcgtcaa
     2641 attgcggcag atattttggc aaggactgac agcaccaata gacgacggca cctgtgcgca
     2701 gaccaagctg cgcccggtcg accgcaaccg gcggaacgtc ggccggctca taatagatcg
     2761 aaagatttgg cagccggatc agctgttcgg aatagtgatc cgctgcgctt tcgggttcca
     2821 tgagatcgct gctgatgaag taatcgatcg tcggaaatcc actcgtgacg ggatgtcccc
     2881 aggaagcgca ttgaaccgcg gcgagccgtt gcgccgccag ttgcgccgac accttgtcca
     2941 tgccgatttc gggaaaaatc agcacatgag gcacgtccgc caatatcgtg cgccgccacg
     3001 catcgagcga tagcgggccc tgcacgaaac gatcgcatat tgtcgccgct gcgtcggttt
     3061 cgctatcgcg gtcggcgctc gtgtaatagc ctgagacgtg aaatcgggtc cggtccagca
     3121 tcttgagcca tcccttgacc ggaatcttcc aattcgagtg ctgcctgaaa aaaccactga
     3181 cgatgcccag cctgatcggt tcgcgcgggc ccggaggatc tggcaaggca gccgtcgtgt
     3241 atctcgcggc catcattctg cacacgagcc gcccatacag ggattgaagc tctcgatcgt
     3301 tacggccctg gtagggcaga taaaacggct gatgggagcc gatcgcctcc gagagaacag
     3361 cgggtgcgcc ggcgctttcc gcatcggcgc agagcctggc cagccgactc gcataagcgg
     3421 cacgccgctc ggcgatttca gatgcgcctt ggtaaatcgc cggaagctcc gccatgcaca
     3481 gggccagttc ggcttgccaa aaatccggct tgagcgcgaa cgcccgggcg aaatgggctc
     3541 tcgcttccgc gatcctggtc tcgccgcgga gcgctacgcc gcaattgtaa tgagccatga
     3601 atgagcccgg atcgatcccg agcgccgcct ggtattgcgc gatcgcctcg tcgcgccgtc
     3661 caagcgcgcg gagaatattg ccaagattgt tgcgcacgcc ggcactgtcc ggtcgcagcg
     3721 cgacggcgcg ctccgcatgc gcgagcgctt caggcagacg atccgtactc tgaagcaggg
     3781 aagccagatg gatgtaagca tcgacaaaat cagggcgcag cgagatcgca cgccgataat
     3841 gcttctcggc gttcggatgt tggccggcca gttcgaacgc cgaggccaga ttgaaatgcg
     3901 cgacagggga ttccggcgtg cttttcagga cccgttcaaa ctgcgccacc gcttcgtcga
     3961 ggcgtccaag cgatcgcaat ccccgcccga gattggtgcg agcctcgcta tatccagggt
     4021 taagcgtcac ggcccgctcg aagcacgata tggcatccgc aaatagtccg cctgctgcga
     4081 gaatcacgcc tctgtcgttg tgcgcctcgg caaaatccgg atcgagcgtc acggcccggc
     4141 cgagatacga cgctgcatcg gggcgtctca attgatgggc gaccaagccc aagagatgaa
     4201 acgcccgggc gttgttggga tcggtatcgc agacgatccg gtagaggcgt tcagcgtcgg
     4261 tgaggcgacc ggcgcgatga tgggccatcg ccgctgcaag cgtctgttcg gagcggcccg
     4321 gcgcgctcat ggcgatatct cgaaccggaa agatctcacg tcagtcgcaa gaccgatagc
     4381 gcccgaggcc gaggtcgacc tcgcaggggc tgccatagac ccagaccggc tgatcgcagg
     4441 gcagcgcgcg cgtcgcccgg tcccgcgtat tacatgcgat gttcgaagcg ggatgaagaa
     4501 ccagccggcc gccgccatag ggagccggca ggacgggttg ttcctgatag cggaccatgt
     4561 agccgtggcg atgatgtctt ggcgccttat ggccattggc ccatccatgg ctgctgccga
     4621 gggcgagaat ggcggcgagc gtgacgatgg ttcgcatcag catgatccct gtcctttaat
     4681 gtctcaagcg ccttcgatcg agaatgcggc cggcgtctgg ccggcaagcc aagtctgcca
     4741 catccgcgca taggccgcct cgatatttcc ggtcaatttt ggcagatcaa ataatgcact
     4801 ggcgtcgcga ttgctctgca gcttctcccg cagcgcgatc aagcgttgcg ggctccgcgc
     4861 cagggccagc gccgcctgtt catattcttc aagcgatttc gtcaccagtt ccggcatgtc
     4921 gatcgccatc agaaggctgc cggcgacacg gcccgcgaag gtgtctccgc agcaggtcaa
     4981 taccggcagt ccggcccaca gcgcatcgct ggccgtggtg tgtgcgttgc acggtaacgt
     5041 gtcgagaaac agatcggcat gccggtggcg tgcgagatgt tcggctggcg gaacgaccgg
     5101 cgcaaagatc agccggccgg aatcgacgcc gcgtttttct gcctcagaac gcagattgcc
     5161 ccggaccagc ggatttgcct cgagcaacca caatacgctg ccggggaccg cgcgaagcag
     5221 acgcatccag atatcgaaga ataccggcga gatcttgtaa ctgttgttga aactgcagag
     5281 caccagcccc tcggctggca gtcca
//
LOCUS       DASWGM010001070         6391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1520871,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001070 DASWGM010000000
VERSION     DASWGM010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520871"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VGA15_28740"
     CDS             <1..715
                     /locus_tag="VGA15_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX9591716.1"
                     /translation="GTYGSRSIAVGGTAIIKAMEKVEAKGKKIAAHLLEASEGDIVIE
                     NGEFKVTGTDKSIALPMVALAAYTAHNLPDGMEPGLKETAFYDPTNFTFPAGAYICEL
                     EVESGTGKTSFVNFVAADDFGRLINPMIVEGQVHGGLAQGIGQALLEGAVYDSNGQLV
                     TASFMDYTMPRADDLPSFKLNHTPTLCPGNPLGVKGCGEAGAIGASAAVINAITDAIG
                     NNKLEMPATPDRVWHAIHG"
     gene            730..1530
                     /locus_tag="VGA15_28745"
     CDS             730..1530
                     /locus_tag="VGA15_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX9591717.1"
                     /translation="MYETTYHRPSSVDEAAALFAKGAEAKYLAGGHTLIPVMKQRLAA
                     PSDVIDLGRIKELVGIDVSADALTIKAATTHYDVVTSGPAQKAIPALAHLASLIGDPA
                     VRYRGTIGGSIATNDPAADYPAAVLALGATVTTNKRSISADDFFKGLFSTALADGEII
                     TQVTFPIPAKAGYSKFPHPASRFALTGVFVVKTKSGDVRVAATGASQNGVMRVSAIEA
                     ALKANWSASALDSVKISAGGLMNDIQGSSDYRANLIKVMAQRAVAAAG"
     gene            1776..3092
                     /locus_tag="VGA15_28750"
     CDS             1776..3092
                     /locus_tag="VGA15_28750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX9591718.1"
                     /translation="MKFGIFYEHQLPRPWNEGDEHRLFQEALDQVELADKLGIDYAWE
                     VEHHFLEEYSHSSAPEIFLAACSQRTKNIRLGHGICLMPPKYNHPARVAERIATLDLV
                     SNGRVEFGTGESASLMELGGFNVGFDPVEKRRMWHEGVEQCTNMMVMDPYPGFKGEFF
                     EMPCRNVVPKPVQKPHPPLWVACSNRETIKMAARHGIGALTFAFVDPREARQWVDDYY
                     RIFKEECVPIGHAVNPNVAMVTGFSVHTDEAESRRRGEDGLRFFRYGLAHHYIFGEHK
                     PGRTDIWANFEKARHALPAAGADHGIGTPDQLRTHLRHFEESGVDQTVFIQQGGRNQH
                     RDICEALELFAKDVMPEFRAREMMREKQKLDELAPYIKKAMERKRAMKPMRDEDIPVY
                     VALGRKVAEEGTGTERQKQNARLWADAAKVTLSGPARHGSKTSATS"
     gene            3124..4236
                     /locus_tag="VGA15_28755"
     CDS             3124..4236
                     /locus_tag="VGA15_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX9591719.1"
                     /translation="MLDQSAPQFATRASGPLAGFRIVEFAGIGPGPFACMMLADMGAE
                     VVTLDRVGARKNLKSVAGRGRKVVELDLKDKAAITQVLNLLANADALIEGFRPGVMER
                     LGLGPEMVQARNPRLVYGRMTGWGQQGPLAQAAGHDINYISVTGALAAIGPAARPVPP
                     LNLVGDFGGGALYLVVGVLAALLEAQKSGKGQVVDAAMCDGAASLMSMFFDMAAAGRW
                     TEARESNFLDGGAHFYGVYECACGRFISIGSIEPQFYALLREHAGLTDADFDKQMDRN
                     AWPELKQKLIDVFKSKTRDDWCAIMEGTDVCFAPILTMNEAPQYPHNAAREIFVTRHG
                     VTQPAPAPRFSRTPSAIRDAAKADIASLTSEWQGGR"
     gene            complement(4417..5535)
                     /gene="hppD"
                     /locus_tag="VGA15_28760"
     CDS             complement(4417..5535)
                     /gene="hppD"
                     /locus_tag="VGA15_28760"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX9591720.1"
                     /translation="MGPFPHDAPAATISADNPMGTDGFEFVEYAHPRPEELHALFKLM
                     GYVPVARHSTKNITVYRQGDINYLVNEEPGTHGCAFLAEHGPCAPSMAFRVVDAKQAY
                     DRALSLGAEPADIPTSQKTLDVPAIKGIGGSLLYFVDRYGAKGSAYDSEFEWLGEQNP
                     RPEGAGLYYLDHLTHNVHRGRMDVWTGFYQKLFNFRQIRFFDIEGRASGLFSRALTSP
                     DGKIRIPINEDAGQSGQIEEYLSVYRGEGIQHIACGAKDIYRTVETLRTDGLPFMPSP
                     PETYFEKIDARLPKHGEDVARLKRDGILIDGEGVVEGGHTKVLLQIFSANAIGPIFFE
                     FIQRKGDDGFGEGNFKALFESIEEDQIRRGVLTLKGAA"
     gene            5655..6131
                     /locus_tag="VGA15_28765"
     CDS             5655..6131
                     /locus_tag="VGA15_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX9591721.1"
                     /translation="MISVDAFDLKILGALQDDGRLTNQQLADIAGLSASQCSRRRVRL
                     EEEQVISGYHADLASEALGFGVIAFIHITLATHSPDNAKRFRTLVNRVADIQEAYSLT
                     GDADYLLKVVLRDLKSLSDMVNNVLMPHQSVAHVRSSIVLDRLKEGSRLPLKELQR"
     gene            6228..>6391
                     /locus_tag="VGA15_28770"
     CDS             6228..>6391
                     /locus_tag="VGA15_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX9591722.1"
                     /translation="MALQLRPNCEYCDKDLPPASMEARICSYECTFCADCVENRLHNV
                     CPNCGGGFVAR"
BASE COUNT         1235 a         1973 c         2010 g         1173 t
ORIGIN      
        1 gggcacttat ggttcgcgct cgatcgcggt cggcggcacc gcgatcatca aggcgatgga
       61 gaaggtcgaa gccaagggca agaaaatcgc ggcgcatctc ttggaggcgt ccgagggcga
      121 catcgtcatc gagaacggcg agttcaaagt caccggcacc gacaagtcga tcgcgctgcc
      181 gatggtcgcg ctcgcggcct acaccgcgca caatctgccc gacggcatgg agccgggcct
      241 gaaggaaacc gcgttctacg atcccaccaa cttcaccttc ccggcgggcg cctatatctg
      301 cgagctcgag gtcgaatccg gcaccggcaa gacctctttc gtcaatttcg tcgccgccga
      361 cgatttcggc cggctgatca atccgatgat tgtcgagggc caggtccatg gcggcctcgc
      421 ccagggcatc ggccaggcgc tgctcgaagg cgcggtttac gactccaacg gccagctcgt
      481 gaccgcgtcg ttcatggact acaccatgcc gcgcgccgac gatctgccgt cgttcaaatt
      541 gaaccacacc ccgacgctgt gtcccggcaa tccgctcggc gtcaagggct gcggcgaggc
      601 cggtgcgatc ggcgcatccg ctgccgtgat caatgccatt accgacgcca tcggcaacaa
      661 caagcttgag atgccggcga cacccgaccg ggtgtggcac gccattcacg gttgagagga
      721 aaacgagcca tgtacgagac aacataccat cgtccctcct cggtcgacga agccgcggcc
      781 ctttttgcaa agggcgcgga ggcgaaatat ctcgccggcg gtcacacgtt gatcccggtg
      841 atgaaacagc gccttgccgc gccgtccgac gtgatcgatc tcggacggat caaggaactg
      901 gtcggcatcg acgtttccgc cgacgccctg accatcaagg ccgcgacgac acactacgac
      961 gtggttacga gcgggcctgc gcaaaaggca attcccgcac tcgcccatct cgcctcgctg
     1021 atcggcgacc cggccgtgcg ctatcgcggc acgatcggcg gctcgattgc cactaacgat
     1081 ccggcggcgg actatccggc cgcggtgctc gcactcggcg cgacggtgac gaccaacaag
     1141 cgttcgatat cggcggatga tttcttcaag ggcctgttct caaccgcgct cgccgacggc
     1201 gagatcatca cgcaggtcac gttcccgatt ccggccaagg cggggtattc caagtttccc
     1261 catccggcgt cgcgctttgc gctgaccggc gtgttcgtgg tcaagaccaa atcaggcgac
     1321 gtccgcgtcg ccgccaccgg cgcctcgcag aacggagtca tgcgggtatc tgcaatcgag
     1381 gcggcgctga aggccaactg gtcggcttcg gcgctggaca gcgtcaagat ttccgcaggc
     1441 gggttgatga acgatattca aggctcgtcg gactatcgcg ccaacctgat caaggtgatg
     1501 gcgcagcgcg cggtcgccgc ggctggatag ccccacttta cgcatggttc gagcgaacgg
     1561 cacgcagcaa cgcgtgccgt tttgcattgg ggcatgcttt tatcggcgat ccgcaggcaa
     1621 attggcgctt gccacgcggc ttgcaaggcg agacaattta gctaatcagc taattaaaac
     1681 gctggtgcaa gatccggaaa aagccggccc cgcgctgatg cggggtggcg ccgttttccg
     1741 aaaagatcat gcgcaaacaa cagggtggag cgcccatgaa gttcggaatt ttctacgagc
     1801 accagctgcc gcggccgtgg aacgagggcg acgagcacag gctgtttcag gaggcgctcg
     1861 atcaggtcga actcgctgac aagctcggca tcgactacgc ctgggaagtc gagcaccatt
     1921 ttctcgagga atattcgcac tcctcggcgc cggaaatatt tcttgcggcc tgctcgcagc
     1981 gcaccaagaa catccgtctc ggccacggca tctgcctgat gccgccgaaa tacaatcacc
     2041 cggcgcgggt tgccgagcgc atcgcgacgc tcgatctcgt ctccaacggc cgcgtcgaat
     2101 tcggcaccgg cgagagcgct tccttgatgg agctcggcgg cttcaatgtc ggcttcgatc
     2161 cggtcgagaa gcggcggatg tggcatgaag gcgtcgagca atgcaccaac atgatggtga
     2221 tggaccccta tcccggcttc aagggcgagt tcttcgagat gccgtgccgc aacgtggtgc
     2281 cgaaaccggt gcagaagcct cacccgccgc tctgggttgc ctgctccaat cgcgagacca
     2341 tcaagatggc ggcgcgtcac ggcatcggcg cgctgacctt tgcgttcgtc gatccccgcg
     2401 aggcccggca atgggtcgat gactactacc ggattttcaa ggaggaatgc gtgccgatcg
     2461 gccacgcggt caatcccaac gtcgcgatgg tgaccggctt ctcggtacac actgatgagg
     2521 cggaatcgcg ccgccgcggc gaggacggtt tgcgtttctt ccgctatggc ctggcgcatc
     2581 attatatctt cggcgagcac aaaccgggcc gcaccgacat ctgggccaac ttcgaaaagg
     2641 cgcggcatgc gttgccggcg gccggcgccg accacggcat cggcacgccg gatcagttgc
     2701 gcacccatct gcggcacttc gaggagtcag gcgtcgacca gaccgtgttc atccagcagg
     2761 gcgggcgcaa ccagcacagg gatatctgcg aagcgctcga gctgttcgcg aaggacgtga
     2821 tgccggaatt tcgggcgcgc gaaatgatgc gggaaaagca gaagctggat gagctcgcgc
     2881 cctacatcaa gaaggcgatg gaacgcaaac gggccatgaa gccgatgcgt gacgaggata
     2941 taccggtcta cgtcgcgctt gggcgaaaag tcgccgaaga aggcaccggc accgaacgcc
     3001 aaaaacaaaa tgccaggctg tgggccgacg ccgccaaggt taccttaagc ggtcccgcgc
     3061 gccacggcag caagacctcc gcgacgtcat agtcctcctt gtctcatcaa cggaaaacac
     3121 aacgtgctcg atcaatcagc cccacagttc gcgacccgcg cgtccggccc gctcgcgggc
     3181 ttccgtatcg tcgagttcgc cggcatcgga cccggtccct ttgcctgcat gatgctggcg
     3241 gacatgggcg cggaagtggt cacgctcgat cgcgtcggcg ccaggaagaa cctgaaatcg
     3301 gtggcggggc gcggacgaaa ggtcgtcgaa ctcgatctga aggacaaggc cgcgattacg
     3361 caggtgctca acctgctggc caatgccgat gcgctgatcg aaggctttcg tcccggcgtg
     3421 atggagcgtc tggggctcgg gcctgaaatg gtgcaggcgc gcaacccgcg cctggtctac
     3481 ggccgcatga ccggatgggg ccagcaaggc ccgctggcgc aggccgcggg ccacgacatc
     3541 aattatatct cggtcaccgg cgcgcttgcc gcgatcggac ctgcggcccg accggtgcca
     3601 ccgctcaatc tggtcggcga tttcggcggc ggcgcgctgt atctggtggt cggcgtgctc
     3661 gccgccctgc tggaggcaca aaagtccggc aagggacagg tggtggacgc cgcgatgtgc
     3721 gacggcgcgg catcgctgat gtcgatgttc ttcgacatgg cggccgcggg ccgatggacc
     3781 gaggcgcgcg agagcaattt cctcgacggc ggcgcgcatt tctacggtgt ctatgaatgc
     3841 gcttgcggcc ggttcatttc gatcggctcg atcgaaccgc aattttatgc gttgctgcga
     3901 gagcacgcag ggctcacgga cgccgatttc gacaagcaga tggatcgcaa tgcctggccc
     3961 gagctgaagc agaagctgat cgatgttttc aagtccaaga cccgtgacga ctggtgtgcg
     4021 atcatggagg gcaccgacgt ctgcttcgcg ccgatcctga ccatgaacga ggcgccccag
     4081 tatccgcaca atgcggcacg cgagatcttt gttacccgcc acggcgtcac ccagccggcc
     4141 ccggcgccgc gcttttcacg cacgccgtcg gcgatcaggg atgcggccaa ggcggatatt
     4201 gcaagcctga cgagtgagtg gcagggcggg cgctaacaag cccgtcgagg cggcccgctc
     4261 tttatccctc cccctcgtgg ggagggtggc atgttgtgag cgaagtgaac aacatgacgg
     4321 gtgggggctg cttccccaaa gtgacttttc gcgcactgcc gtgcgctacc cccacccggc
     4381 gcttcgcgcc accctcccca caagggggag ggatagctac gccgcgccct tcagcgtcag
     4441 cacgccgcgg cggatctggt cttcctcgat cgattcgaac agcgctttga agttgccttc
     4501 gccgaaacca tcgtcgccct tgcgctggat gaactcgaag aagatcggcc cgatggcgtt
     4561 ggcggaaaag atctgcagca gcaccttggt atggccgccc tcgaccacgc cctcgccgtc
     4621 gatcaggatg ccgtcacgct tgaggcgcgc gacgtcctcg ccatgcttcg gcagccgcgc
     4681 gtcgatcttt tcgaaataag tttcgggcgg cgacggcatg aacggcaacc cgtccgtgcg
     4741 cagcgtttcg acagtccgat aaatgtcctt ggcgccgcag gcgatgtgct ggatgccctc
     4801 gccacgatag acgctgagat attcctcgat ctgaccggat tggccggcat cctcgttgat
     4861 cggaatccgg atcttgccgt cggggctggt cagcgcccgc gagaacaggc ccgacgcgcg
     4921 gccctcgata tcgaaaaaac gaatctgccg gaaattgaac agtttttggt agaagccggt
     4981 ccagacgtcc atgcggccgc gatggacgtt gtgagtaagg tgatccaggt aatacagccc
     5041 ggccccctca ggccgcggat tttgttcacc cagccattcg aactcggaat cataggccga
     5101 gcccttggca ccgtagcgat cgacgaagta aagcaggctg ccgccgatac ccttgatcgc
     5161 ggggacatcg agggtcttct gcgaagtcgg aatgtcagcc ggttccgcgc cgagcgacag
     5221 cgcgcgatca taggcctgct ttgcgtcaac cacgcgaaac gccatcgacg gcgcacacgg
     5281 cccgtgctcg gcgaggaagg cacaaccgtg ggtgccgggc tcttcgttga cgagataatt
     5341 gatgtcgccc tggcgataga ccgtgatgtt tttcgtgctg tggcgcgcga ccggcacgta
     5401 gcccatcagc ttgaacagcg cgtgcagctc ttccggcctg ggatgggcgt attcgacgaa
     5461 ttcaaagcca tcggtgccca tggggttatc ggctgaaatc gtcgccgccg gcgcgtcgtg
     5521 cggaaatgga cccatgcttg tctccctgat ttgtcggcac ggatattcgg tcgcctgagg
     5581 cgcaatgtgc atgcaaaaca ttaatatttc ggttatatga tgcacggtct gtgcaggata
     5641 tggatgtttt acgcatgata tcggttgatg ccttcgacct caaaatattg ggcgcgctgc
     5701 aggatgacgg ccgcctgacc aaccagcaat tggccgatat cgccggcctc tcggcctcgc
     5761 agtgctcgcg gcggcgcgtg cggctggagg aagaacaggt gatatcgggc tatcacgccg
     5821 accttgccag cgaagcgctg ggcttcggcg ttatcgcgtt cattcacatc acgctggcga
     5881 cccattcgcc cgacaacgcc aagcggtttc gcaccctggt caaccgcgtc gccgatatcc
     5941 aggaggccta ttcgctgacc ggcgatgcgg actatctgct gaaggtggtg ctgcgcgatc
     6001 tgaaaagcct gtcggatatg gtcaacaacg tgctgatgcc gcaccagagc gtggcgcatg
     6061 tgcgttcgtc gatcgtgctt gatcggttga aggaaggctc aaggctgccg ttgaaggagt
     6121 tgcagcggta aatctgcggg agatggccga aatcgaagga gattcggcat ttgcctctgg
     6181 cgtccgattt gcgatgatcc gcatcgccca gacatcacgg gacgcccatg gcgctgcaac
     6241 tccggccgaa ctgcgagtat tgcgacaagg atcttccgcc agcttcgatg gaagcgcgca
     6301 tctgcagtta cgaatgcacg ttctgtgcgg actgcgtcga gaacaggctg cacaatgtgt
     6361 gcccgaactg cggcggcggt tttgtcgcgc g
//
LOCUS       DASWGM010001071         4295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1523034,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001071 DASWGM010000000
VERSION     DASWGM010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523034"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="VGA15_28775"
     CDS             complement(<1..1540)
                     /locus_tag="VGA15_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX9591723.1"
                     /translation="MAIAFTLPRISGFALALLAFTMMAGARGQDAPAPPAQQPEAAPP
                     GQKSGAAGGRAATPNSPASPTSPATAEAHRLPPDSTTKQTLALPGRTLNFAATAGSIR
                     LFDDKGEPQADIAYTAYQLDGADPRSRPVTFLFNGGPGAASAYLQFGNAGPWRLAIGG
                     DAAVSSAPPDLMPNAETWLDFTDLVFIDPVGTGYSRFVATGEDVRKRFFSIDGDVNAI
                     ALTIRRWLEKYDRLLSPKFVAGESYGGIRGPKIVHELQTEQGVGVRGLILVSPLMDFR
                     EYSGSSILQYVASLPTMAAVAREAKAAVTRADMADVEGYARGDFLSDLVKGTADASAT
                     TRLADRVAALTGIDQAVSRRLAGRFDVAEFRREFDRRDGKVTGRYDASVLGFDPHPDS
                     SFYRFEDPSGDPLIAPLTSAAVEFTTRKLNWRPDGSYQLLNGAVEKAWDFGRGRAPAE
                     SISQLRQILALDPKLKLLVGHGLFDLATPYFGSKILLDQLPAYASPERVKLVVYPGGH
                     MFYSR"
     gene            1826..2605
                     /locus_tag="VGA15_28780"
     CDS             1826..2605
                     /locus_tag="VGA15_28780"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX9591724.1"
                     /translation="MTTPQRYERIAFIASAGAEAQQALAQLSELYGNHDPADADIVVA
                     LGGDGLMLQTLHQHMRSGKPIYGMHRGTVGFLMNEFSPIDLHARLAAARESVIHPLLM
                     RATDVHGAVHLHHAINEVALFRQTHQAARLRILIDERERMSELIADGIMVATPAGSTA
                     YNLSAQGPILPINAALLALTPICAFRPRRWRGALLPNTAFVVIEVLEGDKRPVAAVAD
                     HDEARDVRRVEVLSDKTISMRMLFDPGHSLEERILSEQFGY"
     gene            2729..3274
                     /locus_tag="VGA15_28785"
     CDS             2729..3274
                     /locus_tag="VGA15_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX9591725.1"
                     /translation="MFRIDFNKLRFLVCDDNPHMRRILRTLLHSFGAREVYESEDGAT
                     ALEMYSHYVPDIVITDWSMPIFDGLELAQMIRQPESKGNPYAPIIMLTGHSEKRRVTV
                     ARDAGVTEFLAKPISAKGLYQRILNVVANPRPFIKTKTYFGPDRRRNTANAYIGPERR
                     LGGEAEILPQPSLLDKARTTV"
     gene            3314..3892
                     /locus_tag="VGA15_28790"
     CDS             3314..3892
                     /locus_tag="VGA15_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX9591726.1"
                     /translation="MTKDKPGTVQVKTFADHKVITQPNPLRKVLRRVADKDLDDPIAR
                     AEKALAGLSGEFKNWMSVEADRLAAAHTAILKNGFTDFTREELFRAAHDIKGDAATFG
                     FPSAAAAAESLCRIIEHAPNLDEVPSDLIEHHINAIQAIVRERTRLDTVSTANELSRQ
                     LRGVADEFLKHANRDRPEHLEAILAPSIVPAD"
BASE COUNT          819 a         1403 c         1317 g          756 t
ORIGIN      
        1 cgcgcgaata gaacatgtgg ccgcccggat agaccacgag ctttacacgc tcgggcgaag
       61 cataggccgg cagctgatcg agaaggattt ttgagccgaa atagggcgtg gcgagatcga
      121 acaggccgtg ccctaccagc aatttcaatt tcgggtcgag ggcgagaatc tgccgcagct
      181 gggaaatcga ctcggcggga gcgcggccac ggccgaaatc ccaggccttc tcgaccgcgc
      241 cgttgagcag ctggtaggat ccgtccggcc gccaattgag cttgcgcgtg gtgaattcga
      301 ccgcagcact ggtcaacggc gcgatcaggg gatcaccgga gggatcttcg aaacgataaa
      361 aactcgaatc cgggtgggga tcgaaaccca gcaccgaggc gtcgtaacgt cccgtcacct
      421 tgccgtcgcg ccgatcgaat tcgcgccgga actcggcaac gtcgaagcgc ccggccagcc
      481 tgcggctcac cgcctgatcg atcccggtca gcgccgccac ccggtcggca agccgcgtag
      541 tggccgaggc gtcggctgtg cccttgacga gatcgctcaa aaaatcgccg cgcgcgtaac
      601 cttcgacatc ggccatgtcg gcgcgggtca cagcagcttt ggcctcgcgc gcgaccgccg
      661 ccatggtggg aaggctcgcg acatattgca ggatgctgga gcccgaatat tcgcggaaat
      721 ccatcagcgg agagaccagg atgagacctc tcaccccgac gccctgctcg gtctgcaatt
      781 cgtgaacgat cttcggcccg cggatgccgc catagctttc accggcgacg aatttgggcg
      841 acagcaagcg gtcgtacttt tcgagccagc ggcggatcgt cagcgcgatc gcgttgacgt
      901 cgccgtcgat cgagaaaaac cgcttgcgca cgtcctcgcc ggtcgcgacg aagcggctgt
      961 aaccggtgcc gaccggatca atgaagacga gatcggtaaa gtcgagccag gtctcggcgt
     1021 tgggcataag atcgggcggc gccgaggaga ccgccgcgtc accaccgatg gccagccgcc
     1081 agggtccggc attgccgaat tgcaggtagg cggaagcagc ccccggcccg ccattgaaaa
     1141 ggaacgtcac cggccggctg cgcggatccg caccatcgag ctggtaggcg gtgtaggcga
     1201 tatcggcttg cggctcgccc ttgtcgtcga acaggcggat cgagcccgcg gtcgcggcaa
     1261 agttgagcgt gcggcccggc aacgccagcg tttgtttggt ggtcgaatcc ggcggaaggc
     1321 gatgcgcttc ggctgtggcc ggcgaagttg gcgaagctgg cgaatttggc gtcgcggcgc
     1381 gtccgcccgc tgcacccgat ttctgacccg gaggcgccgc ttcgggttgc tgcgcgggcg
     1441 gcgcaggcgc atcctgcccg cgcgcgccgg ccatcatggt gaacgcgagc agcgccagcg
     1501 cgaagcccga aatacgcggc aaggtgaaag cgatcgccat atccgttgtt ctccacgctt
     1561 cccgcgttgt tctccacgct tccctacaat cgacccggcg cgacacatta atcctatgcg
     1621 cggcattttc tggatcgagg cgccgttcta gcgctaaatt acgacaagtt ggcatggttc
     1681 actaaaagtt ggcatggtca ctagctcgcc gtcccctgcc gtcactggct aaccggccta
     1741 tctcgacaag cccggccaag gtcgtataga cgaccatccg cgcaatcggt ttccctttcc
     1801 gcgccaacat gagcccgcaa tccccatgac cacgccccag cgatatgaac ggattgcctt
     1861 cattgcgagc gccggcgcgg aggcccagca ggcgcttgcg caactgagcg agctctacgg
     1921 caatcacgat cccgccgatg ccgacatcgt ggtggcgctc ggcggcgatg gcttgatgct
     1981 gcagacgctg caccagcaca tgcgctcggg caagccgatc tacggcatgc atcgcggcac
     2041 cgtcggtttt ctgatgaacg agttcagtcc gatcgatctg catgcccgtc tggccgccgc
     2101 aagggaatcc gtcattcatc cgcttttgat gcgcgccacc gacgtgcatg gcgcggtgca
     2161 tctgcatcat gccatcaacg aggtcgcgct gtttcgccag acccatcagg cggcgcgcct
     2221 gcggatcctg atcgacgagc gcgagcgcat gtccgagttg atcgccgacg gcatcatggt
     2281 ggcgacgccg gcgggatcca ccgcctataa tctgtcggcg caggggccga tcctccccat
     2341 caacgcggcg ctgctggcgc tgaccccgat ctgcgcgttc cggccgcggc gctggcgcgg
     2401 cgccctgctg ccgaacaccg cctttgtggt gatcgaagtg ctggagggcg acaagcggcc
     2461 ggtcgccgcc gtcgccgacc acgacgaggc gcgcgatgtc cgccgcgtcg aggtgctctc
     2521 cgacaagacc atctcgatgc ggatgctgtt cgatcccggc cacagcctgg aagagcgcat
     2581 tttgagcgag cagttcggct actgattttg ctctcccccg tcgacgacgg gcgaagccga
     2641 cagcaaaatc gcaaccattc gttaacccgc gccgcgatat ggttaacata ggtctaccgt
     2701 ctcaaaggta tgccctcggg gccgggccat gtttcgcatc gatttcaaca agctgcgatt
     2761 tctcgtatgc gacgacaatc cgcatatgcg ccgcattttg cggacgctgc tgcattcgtt
     2821 cggcgcgcgc gaggtctatg aatccgagga cggcgccacc gcgctcgaaa tgtacagcca
     2881 ctacgttccc gatatcgtca tcaccgactg gtcgatgccg attttcgacg gcctcgagct
     2941 tgcccagatg atccgccagc cggaatccaa gggcaatccc tacgctccca tcatcatgct
     3001 gaccggccat tccgaaaagc gccgcgtcac ggtcgcgcgc gacgccggcg tcaccgagtt
     3061 tttggccaag ccgatctcgg ccaaggggct ctatcagcgc attctgaatg tggtcgccaa
     3121 tcccagaccg ttcatcaaga caaaaaccta ttttggcccc gaccgccggc gcaacaccgc
     3181 caacgcctat atcggccccg agcgccgcct cggcggcgaa gcggaaatcc ttccgcagcc
     3241 gtcgctgctc gacaaggcaa gaactaccgt ttagcggacg actgtttgag ctgcgcgcga
     3301 caaggaagcc atgatgacca aagacaagcc cgggacggta caagtaaaaa ccttcgccga
     3361 ccacaaggtg atcacgcagc ccaatccgtt gcgcaaggtg ctgcggcggg tcgccgacaa
     3421 ggatctcgac gatcccatcg cgcgggcgga aaaggccctc gctggcctgt ccggcgaatt
     3481 caagaactgg atgtcggtcg aggccgaccg gctcgcggcg gcccacaccg ccattctcaa
     3541 aaacggcttt acggatttca cccgcgaaga gctgtttcgc gccgcccatg acatcaaggg
     3601 cgacgccgcc acgttcggtt tcccttcggc cgctgccgcc gccgagagcc tgtgccggat
     3661 catcgaacac gcgcccaatc tcgacgaggt gccgtccgac ctgatcgaac atcatatcaa
     3721 cgccatccag gccatcgtgc gcgagcgcac caggctcgac accgtctcga ccgcgaacga
     3781 actgagccgg caattgcgcg gcgtcgccga cgaatttctc aagcacgcca accgcgaccg
     3841 ccccgagcac ctcgaagcca ttcttgcgcc gagcattgtg ccggcggatt gagagagatc
     3901 tttcattgcg tcgtcccggc gaaggccggg acccataatc cctggcgggt gtggttgcgg
     3961 aaggtgtctg ctttcgtgcc tcatcgagac gacacggctt atgggtcccg gccttcgccg
     4021 ggacgacgta gagtgtgtgg cgttttcaaa cagcgcgctg ctgttccctc ccccttgcgg
     4081 gggagggtta gggagggggg tagccgcaag gtcggtgctc gtggctaccc ccctctccaa
     4141 ctctcccccg caagggcagg gctgtcccgg gaatgattca ctatgccgca gcgcatgccc
     4201 ggcaaatcca gcgtgaaccg gatactttct ggttgtcgag acacagaaaa gggccgggca
     4261 tgcaatacgt gaatagcatc tttggggagc tcctc
//
LOCUS       DASWGM010001072         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U2816 k141_1384055,
            whole genome shotgun sequence.
ACCESSION   DASWGM010001072 DASWGM010000000
VERSION     DASWGM010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960463
            Sequence Read Archive: SRR10360654
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 06:50:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,760
            CDSs (total)                      :: 5,739
            Genes (coding)                    :: 5,615
            CDSs (with protein)               :: 5,615
            Genes (RNA)                       :: 21
            tRNAs                             :: 17
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 45 of 124
            Pseudo Genes (incomplete)         :: 85 of 124
            Pseudo Genes (internal stop)      :: 12 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384055"
                     /isolate="SMAG_U2816"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="39.7292 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /gene="chvE"
                     /locus_tag="VGA15_28795"
     CDS             <1..838
                     /gene="chvE"
                     /locus_tag="VGA15_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX9591727.1"
                     /translation="ENMVTKGAKVLVIASIDGTTLSDVLKQAKDKGITVIAYDRLIRD
                     TPNVDYYATFDNFQVGVLQAQSIEQGLKLKEGKGPFNIELFGGSPDDNNAYFFYNGSM
                     SVLQPYIDSGKLVVVSRQTGMDKVSTLRWDGATAQARMDNLLSAFYTTKRLDAVLSPY
                     DGLSIGILSSLKGVGYGGGNMPMPIVTGQDAEVPSIKSMLRGEQYSTIFKDTRDLAKV
                     TADMVDAALSGKTVTVNDTTTYKNGVKVVPSYLLKPVVVDKTNWEKVLIDSGYYKKAQ
                     VN"
     gene            1082..>2434
                     /locus_tag="VGA15_28800"
     CDS             1082..>2434
                     /locus_tag="VGA15_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX9591728.1"
                     /translation="MTTLLDMRGISKAFVGVKALSDVNFTVRQGEIHALVGENGAGKS
                     TLMKVLSGVYPSGSYDGSIVYDGEERHFRDINDSEALGIIIIHQELALIPLLSIAENI
                     FIASPPSRFGVIDRNAVYRRSQQLLAKVGLNEAPDTLVTNIGIGKQQLVEIAKALSKE
                     VRLLILDEPTASLNESDSAALLDLLLEFRSQGMSAILISHKLNEVARVADRITVLRDG
                     RTVDTLDCKAGAVEEDRIIRKMVDRDLEHRFPKREPNIGDPVFRVENWSVYHPLHADR
                     QVIKNVDFHVRRGEVVGIAGLMGAGRTEFAMSLFGRSWGRKITGRAMLHGREIDLSTV
                     SRAIDAGLAYVTEDRKQFGLLLADDVRKNITLANLGSVATGRVIDDIKELRVANDYRN
                     RMRIRCSDVYQTSGNLSGGNQQKVVLSKWLFTDPEILILDEPTRGIDIGAKYEIYCII
                     N"
BASE COUNT          499 a          727 c          750 g          458 t
ORIGIN      
        1 cgagaacatg gtgacgaagg gcgccaaggt gctggtcatc gcttcgatcg acggcaccac
       61 cctgtccgat gtgctcaagc aggccaagga caagggcatt acggtgatcg cctacgaccg
      121 gctgatccgc gacacgccca atgtggatta ctatgcgacc ttcgacaact tccaggtcgg
      181 cgtgctgcag gcgcagtcga tcgagcaggg gctgaagctg aaggaaggca agggcccgtt
      241 caacatcgag ctgttcggcg gctcacccga cgacaacaac gcttactttt tctacaacgg
      301 ctcgatgtcg gtgttgcagc cctacatcga cagcggcaag ctcgtggtcg tcagccgcca
      361 gaccggcatg gacaaggtct cgacgttgcg ctgggatggc gccacggcgc aagcccgcat
      421 ggataatctg ttgagcgcgt tctataccac caagcggctc gacgccgtgc tctcgcccta
      481 tgatgggctt tcgatcggca tcctgtcgtc gctcaagggc gtcggctatg gcggcggcaa
      541 catgccgatg ccgatcgtca ctggtcagga tgcggaagtg ccttctatca aatcgatgct
      601 gcgcggcgag cagtattcga cgatcttcaa ggacacccgc gacctcgcca aggtcaccgc
      661 cgacatggtg gacgcggcac tgagcggcaa gaccgtgacg gtgaacgaca ccaccactta
      721 caagaatggc gtgaaggtgg tgccgtccta tctactcaag ccggtggtag tcgataagac
      781 caactgggag aaagttctga tcgacagcgg ctattacaag aaggcgcagg tcaactgagg
      841 ctggctgtaa cgaagcgtgg aggcgatcct ttcgccatgg ctcggcaaca agcgccaact
      901 ggtccgcgca agcgcggccc gatgacaggc tccgcgcgtc ttcgcgctag ctgcccggcc
      961 atccatgccc cccttgcggc aagtctgcga agatgtggat gcccgtagca agtgtaacaa
     1021 gtgtgggcat ggtgacggtg gctggtcttg ttcaaagtca ccatgtctcc agggagttgg
     1081 aatgacaaca ctgctcgaca tgcgcgggat cagcaaagcc ttcgtcggcg tgaaggcctt
     1141 gagcgacgtc aatttcaccg tcaggcaggg cgagatccat gctttggtgg gcgagaacgg
     1201 cgccggcaag tcgacgttga tgaaggtgtt gagtggcgtc tatcccagcg gcagttatga
     1261 tggcagcatc gtctatgacg gcgaggagcg gcatttccgc gacatcaatg attccgaggc
     1321 gctcggcatc atcatcattc atcaggagct cgcgttgatc ccgctgctct cgatcgcgga
     1381 gaatattttt atcgccagcc cgccgtcgcg gttcggcgtg atagatcgca acgcggtcta
     1441 caggcgctcg cagcagctgc tggcgaaagt ggggctcaat gaggcgccgg acacgctggt
     1501 caccaatatc gggatcggca agcagcagct ggtggagata gccaaggcgc tgtccaagga
     1561 ggtgcgcctc ttgattctcg acgagccgac cgcgagcctc aacgaaagcg acagcgcggc
     1621 gctgctcgat cttttgcttg agttccgctc ccagggcatg tcggcgatcc tgatctcaca
     1681 caagctcaac gaggtggcgc gtgttgccga ccgcatcacc gtgctgcgcg acggtcgcac
     1741 cgtcgatacg ctggattgca aggccggcgc cgtcgaggag gatcgcatca tccgcaagat
     1801 ggtggatcgc gacctcgagc atcgcttccc gaagcgcgaa ccgaacatcg gcgatccggt
     1861 gttcagggtc gagaactggt cggtctacca tccgctgcat gcggatcggc aagtcatcaa
     1921 gaatgtcgat ttccatgtcc gccgcggtga agtcgtcggc atcgctgggt tgatgggcgc
     1981 cgggcgcacc gaattcgcca tgagtctgtt cggccgctca tggggccgca agatcaccgg
     2041 ccgcgccatg ctgcacggcc gcgagatcga tctctcgacc gtcagccgcg ccatcgacgc
     2101 cggccttgcc tacgtcaccg aagaccgcaa gcagttcggc ctcctgctcg ccgacgatgt
     2161 gcgcaagaac atcacgcttg ccaatctcgg cagcgtcgcg accggccgcg tcatcgacga
     2221 catcaaggag ttgcgggtgg cgaacgatta tcgcaaccgc atgcgcatcc gttgctccga
     2281 tgtctatcag acttccggca acctgtccgg cggcaaccag caaaaggtgg tgctgtcgaa
     2341 atggctgttc accgatccgg aaatcctgat cctggacgaa ccgacccgcg gtatcgacat
     2401 cggcgccaag tacgagatct attgcatcat caac
//
